LOCUS       DAZFFE010000001       130289 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFFE010000001 DAZFFE010000000
VERSION     DAZFFE010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 130289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 130289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..130289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.001"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            155..490
                     /gene="yjdM"
                     /locus_tag="ACHJ69_000001"
     CDS             155..490
                     /gene="yjdM"
                     /locus_tag="ACHJ69_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO5013088.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            650..1093
                     /gene="yjdN"
                     /locus_tag="ACHJ69_000002"
     CDS             650..1093
                     /gene="yjdN"
                     /locus_tag="ACHJ69_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO5013089.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTH"
     gene            1226..2014
                     /gene="phnC"
                     /locus_tag="ACHJ69_000003"
     CDS             1226..2014
                     /gene="phnC"
                     /locus_tag="ACHJ69_000003"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO5013090.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSVGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGCSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2039..3055
                     /gene="phnD"
                     /locus_tag="ACHJ69_000004"
     CDS             2039..3055
                     /gene="phnD"
                     /locus_tag="ACHJ69_000004"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5013091.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLNPAYAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKDNKGLNEQDKLAKTTAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3161..3940
                     /gene="phnE"
                     /locus_tag="ACHJ69_000005"
     CDS             3161..3940
                     /gene="phnE"
                     /locus_tag="ACHJ69_000005"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO5013092.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVIAVLIVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            3961..4686
                     /gene="phnF"
                     /locus_tag="ACHJ69_000006"
     CDS             3961..4686
                     /gene="phnF"
                     /locus_tag="ACHJ69_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO5013093.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     CFDSGSLHDFLREQTGITLRRSQTRISARRAQAKECQHLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4687..5139
                     /gene="phnG"
                     /locus_tag="ACHJ69_000007"
     CDS             4687..5139
                     /gene="phnG"
                     /locus_tag="ACHJ69_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO5013094.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNINADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQNRHFQNLSETLITPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5136..5720
                     /gene="phnH"
                     /locus_tag="ACHJ69_000008"
     CDS             5136..5720
                     /gene="phnH"
                     /locus_tag="ACHJ69_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO5013095.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVNQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVTSLSGGRMLRLTGAGIAEERMIAPQLPKCILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5720..6784
                     /gene="phnI"
                     /locus_tag="ACHJ69_000009"
     CDS             5720..6784
                     /gene="phnI"
                     /locus_tag="ACHJ69_000009"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO5013096.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLE
                     RRISAIYKDIPGGQLLGPTYDYTHRLLDFTLLANGETPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDIFIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6777..7622
                     /gene="phnJ"
                     /locus_tag="ACHJ69_000010"
     CDS             6777..7622
                     /gene="phnJ"
                     /locus_tag="ACHJ69_000010"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO5013097.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQN
                     EAKSQ"
     gene            7619..8377
                     /gene="phnK"
                     /locus_tag="ACHJ69_000011"
     CDS             7619..8377
                     /gene="phnK"
                     /locus_tag="ACHJ69_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO5013098.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVMELNLAVVIVTHDLGVARLLADRLLV
                     MKQGKVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8488..9168
                     /gene="phnL"
                     /locus_tag="ACHJ69_000012"
     CDS             8488..9168
                     /gene="phnL"
                     /locus_tag="ACHJ69_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO5013099.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQGASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGTAIVGIFHDETVREQVADR
                     LHPMGVSS"
     gene            9165..10301
                     /gene="phnM"
                     /locus_tag="ACHJ69_000013"
     CDS             9165..10301
                     /gene="phnM"
                     /locus_tag="ACHJ69_000013"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO5013100.1"
                     /translation="MIINNVKLVLENEVVHGSLEMQDGEIRAFAESQSHLSEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAM
                     CRARHIALASHDDATHAHVAQSHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10301..10858
                     /gene="phnN"
                     /locus_tag="ACHJ69_000014"
     CDS             10301..10858
                     /gene="phnN"
                     /locus_tag="ACHJ69_000014"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO5013101.1"
                     /translation="MTGKLIWLMGPSGSGKDSLLAELRQREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10845..11279
                     /gene="phnO"
                     /locus_tag="ACHJ69_000015"
     CDS             10845..11279
                     /gene="phnO"
                     /locus_tag="ACHJ69_000015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO5013102.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHQAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11281..12039
                     /gene="phnP"
                     /locus_tag="ACHJ69_000016"
     CDS             11281..12039
                     /gene="phnP"
                     /locus_tag="ACHJ69_000016"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO5013103.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECVACARARRSPQYRRQPCSGVV
                     KFNEAITLIDAELHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGNQIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIEVE"
     gene            complement(12067..12396)
                     /gene="yjdP"
                     /locus_tag="ACHJ69_000017"
     CDS             complement(12067..12396)
                     /gene="yjdP"
                     /locus_tag="ACHJ69_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO5013104.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12465..12914)
                     /gene="rpiB"
                     /locus_tag="ACHJ69_000018"
                     /gene_synonym="alsI"
     CDS             complement(12465..12914)
                     /gene="rpiB"
                     /locus_tag="ACHJ69_000018"
                     /gene_synonym="alsI"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HFO5013105.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERDVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEADGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQQRN"
     gene            13273..14163
                     /gene="alsR"
                     /locus_tag="ACHJ69_000019"
     CDS             13273..14163
                     /gene="alsR"
                     /locus_tag="ACHJ69_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose utilization transcriptional regulator
                     AlsR"
                     /protein_id="HFO5013106.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            14222..15157
                     /gene="alsB"
                     /locus_tag="ACHJ69_000020"
     CDS             14222..15157
                     /gene="alsB"
                     /locus_tag="ACHJ69_000020"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HFO5013107.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            15284..16816
                     /gene="alsA"
                     /locus_tag="ACHJ69_000021"
     CDS             15284..16816
                     /gene="alsA"
                     /locus_tag="ACHJ69_000021"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HFO5013108.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHDTVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNIAISRSLKDGGYKGAMGLFHEVDEQRTAENQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCRPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            16795..17775
                     /gene="alsC"
                     /locus_tag="ACHJ69_000022"
     CDS             16795..17775
                     /gene="alsC"
                     /locus_tag="ACHJ69_000022"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HFO5013109.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSS
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGI
                     DPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            17786..18481
                     /gene="alsE"
                     /locus_tag="ACHJ69_000023"
     CDS             17786..18481
                     /gene="alsE"
                     /locus_tag="ACHJ69_000023"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HFO5013110.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHGMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKVAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            18465..19394
                     /gene="alsK"
                     /locus_tag="ACHJ69_000024"
     CDS             18465..19394
                     /gene="alsK"
                     /locus_tag="ACHJ69_000024"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0019316 - D-allose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HFO5013111.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKQTAEVIAPD
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDGVILGGGVMDMPAFPRETLIAMTQKYLRRPLPYQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQSCAKVP"
     gene            19666..21651
                     /gene="yjcS"
                     /locus_tag="ACHJ69_000025"
     CDS             19666..21651
                     /gene="yjcS"
                     /locus_tag="ACHJ69_000025"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO5013112.1"
                     /translation="MNNSRLFRLSRIVIAFTAASGMMINTAYATDEAKAATQYTQQVN
                     QNYAKSLPFGDRQDFDDAQRGFIAPLLDEGILRDANGKIYYRANDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMAVRLDSTKA
                     AGKNISLNFNMSNGDNLNLMLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            21860..22135
                     /gene="ytcA"
                     /locus_tag="ACHJ69_000026"
     CDS             21860..22135
                     /gene="ytcA"
                     /locus_tag="ACHJ69_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO5013113.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPGWF
                     FCAIASLILTLITRRIIQRTNINLAFVGIIYTALFALYAMLFWLAFF"
     gene            22154..23185
                     /gene="mdtN"
                     /locus_tag="ACHJ69_000027"
     CDS             22154..23185
                     /gene="mdtN"
                     /locus_tag="ACHJ69_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO5013114.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQASDTLRRTEPLLREGFVSAEEVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTLATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            23185..25236
                     /gene="mdtO"
                     /locus_tag="ACHJ69_000028"
     CDS             23185..25236
                     /gene="mdtO"
                     /locus_tag="ACHJ69_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO5013115.1"
                     /translation="MSALNSLPLPVVRLLTFFHEELSERRPGRVPQTMQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIASLFLIYKWSYGEP
                     LIRLIIAGPLLMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRIQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINNLQHSITEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYNPVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVVLERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGV
                     ATALSHSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            25233..26699
                     /gene="mdtP"
                     /locus_tag="ACHJ69_000029"
     CDS             25233..26699
                     /gene="mdtP"
                     /locus_tag="ACHJ69_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO5013116.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQFNDPQLDALIQRTLSGSHPLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            26897..29044
                     /gene="fdhF"
                     /locus_tag="ACHJ69_000030"
     CDS             26897..29044
                     /gene="fdhF"
                     /locus_tag="ACHJ69_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:27314..27316,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HFO5013117.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAESAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRVAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            29419..30987
                     /locus_tag="ACHJ69_000031"
     CDS             29419..30987
                     /locus_tag="ACHJ69_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5013118.1"
                     /translation="MIKKLLPLLVLSTISATTVAATPPNTLVVAQGLDDIVSLDPAEA
                     NELSSIQTVPSLYQRVVQPDRNDPTKITPILAESWQADPAAKTLTIKLKSDAKFASGN
                     PVRPEDVIYSYVRAVTLNKSGAFILNVLGWQPENIASQLKKIDDHTVQVQWSADVSPA
                     LALNILSTPIASIVDEKLVAPNAKNNDFGNEWLKMHSAGSGAFKMRTYQPHQAIVMEA
                     NTSSPTGAPKLKSVIIKNVPDPATRRLLIQQGDADMARDLGADQIDALQGKPGVKVMS
                     IASAEQNYLAFNTGNKDNPLMSNPAFWEAARWLVDYDGITKNLLKGQYFTHQSFLPVG
                     FPGALEETPFTFNPAKAKEILAKAGIKDPHFTLDVENKPPFITIAQSIQASFAQGGVK
                     VDLLPAAGSQVYSRVRAHQHQGAIRMWLPDYFDAHSNASSFAYNDGKSSTVAGLNGWK
                     IPELNKETLAAIAEPDSAKRLDLYKKMQQELQRSSPYVFIDQGKTQIVMRDNVQGYQQ
                     GLNADMVWFDQVTK"
     gene            31087..32136
                     /locus_tag="ACHJ69_000032"
     CDS             31087..32136
                     /locus_tag="ACHJ69_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5013119.1"
                     /translation="MSVVFSTRARWRGKRPLLALLQGLFTVALTLLGLLLITFALSAL
                     SPVDRVLQIVGDHASQSTYDQVRHQLGLDQPLAVQFWHYLMNLAHGDLGIASSTVQPV
                     LQDLLSAFPATIELATLALIVGAVVGVIAGVLCARYVGSPLDFTVRTLTLLGNSVPVF
                     WLGLLMLALFYARLQWSAGPGRLDDIYQFTIEPRSGFALIDTWLSGDREAFTNAINHL
                     ILPVLLLAYFSLASITRLTRSACLGEMNKEYILLARAKGASEMTILLRHVLPNIRGTL
                     LTVIALAYTSMLEGAVLTETVFSWPGIGRYLTTALFAGDTTAIMGGTLLIGVCFVLIN
                     NLTDLLVRLTDPRLR"
     gene            32136..32969
                     /locus_tag="ACHJ69_000033"
     CDS             32136..32969
                     /locus_tag="ACHJ69_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5013120.1"
                     /translation="MPMFLFLRRLRHSPAAFCGLIIILLLMLIALFAPWLAPHDPNWQ
                     DAAARLQAPSRQHWLGTDSYGRDLLSRLIYGSRPALGLVALVTIITLPVGLLIGILAG
                     YYGGWLERVLMRFTDVVMSMPRLILAFAFVAMLGPGLVNGALALALTTWPAYARQARS
                     EIQRLRHSDYLAAAEMMGIRGRRLLAGHILPLCLPSAIVRLALDLAGIILAAAGLGFL
                     GLGARPPMAEWGAMIADGMQVIFDQWWIAAAPGAIILIASLAFNLLGDGLRDVLEPQH
                     D"
     gene            32962..33798
                     /locus_tag="ACHJ69_000034"
     CDS             32962..33798
                     /locus_tag="ACHJ69_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5013121.1"
                     /translation="MTDSRLSINNLCVDYPESRVVNNVSFTLGNERLALVGESGSGKS
                     MTARALMGLVRKPGVVTADTLNILGRDALTLNARGWQQLRGNDIAMVLQDPRYALNPV
                     KTVKAQLEEALTLHQRLNRREKEEKINTAIQAVGLNTRVLQSYPRELSGGMGQRVMIA
                     IALINDPRVLIADEPTSALDVRLRNQILELLVTQCEQRQMAMLLISHDLPLVAQFCHR
                     VLVMYQGNKVDEMHAAALPTATHPYTRTLWTCRPNAQTYGQMLPTLDRTAMTPEKYHD
                     DC"
     gene            33785..34489
                     /locus_tag="ACHJ69_000035"
     CDS             33785..34489
                     /locus_tag="ACHJ69_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5013122.1"
                     /translation="MTIVEINHLQVTFAEKTAVSAASFSVNAGETFSLIGESGCEKST
                     ILRVLAGLQRDWHGQVSLFGHTIKPGMRFQGDLRRNVQMVFQDPYASLHPNHTLWRTL
                     AEPLKIRGEREIEKRVQTALEQVGLPFDAARRYPHQLSGGQRQRVVIARALLLRPQLL
                     LLDEPTSALDMSVQAEILNLLNQLKLEHQMTYLLVSHDADVIAHMSDRAALMSEGKIQ
                     RFFDRDAMEKGEHRMD"
     gene            34600..35289
                     /gene="yjcO"
                     /locus_tag="ACHJ69_000036"
     CDS             34600..35289
                     /gene="yjcO"
                     /locus_tag="ACHJ69_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO5013123.1"
                     /translation="MKKIITLMLFLTFFAHANDSEPGSQYLKAAEARDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(35435..36748)
                     /gene="gltP"
                     /locus_tag="ACHJ69_000037"
     CDS             complement(35435..36748)
                     /gene="gltP"
                     /locus_tag="ACHJ69_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO5013124.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(37091..37687)
                     /gene="nrfG"
                     /locus_tag="ACHJ69_000038"
     CDS             complement(37091..37687)
                     /gene="nrfG"
                     /locus_tag="ACHJ69_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO5013125.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTVQARAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(37684..38067)
                     /gene="nrfF"
                     /locus_tag="ACHJ69_000039"
     CDS             complement(37684..38067)
                     /gene="nrfF"
                     /locus_tag="ACHJ69_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO5013126.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQEQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYTMVEEGKSEVEIIDWMTERYGDFVRYNPPLTGQT
                     LVLWALSVVLLLLMALILWRVRAKR"
     gene            complement(38060..39778)
                     /gene="nrfE"
                     /locus_tag="ACHJ69_000040"
     CDS             complement(38060..39778)
                     /gene="nrfE"
                     /locus_tag="ACHJ69_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO5013127.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLATLLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRSEFDGVSARICWRWARPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFALLMLVVIVLATFVSGKRAQLPALLAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            complement(39798..40754)
                     /gene="nrfD"
                     /locus_tag="ACHJ69_000041"
     CDS             complement(39798..40754)
                     /gene="nrfD"
                     /locus_tag="ACHJ69_000041"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO5013128.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVRKVLSLLTPVHRGLETLMLVLAALLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(40751..41422)
                     /gene="nrfC"
                     /locus_tag="ACHJ69_000042"
     CDS             complement(40751..41422)
                     /gene="nrfC"
                     /locus_tag="ACHJ69_000042"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO5013129.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACRKVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTHL
                     QAGKLPACVEACPTKALTFGNLDDPSSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(41419..41985)
                     /gene="nrfB"
                     /locus_tag="ACHJ69_000043"
     CDS             complement(41419..41985)
                     /gene="nrfB"
                     /locus_tag="ACHJ69_000043"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO5013130.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(42030..43466)
                     /gene="nrfA"
                     /locus_tag="ACHJ69_000044"
     CDS             complement(42030..43466)
                     /gene="nrfA"
                     /locus_tag="ACHJ69_000044"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO5013131.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPATPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            43858..45816
                     /gene="acs"
                     /locus_tag="ACHJ69_000045"
     CDS             43858..45816
                     /gene="acs"
                     /locus_tag="ACHJ69_000045"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO5013132.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWITPYQKVKNTSFAPGNVSIQWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSSSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHNLVEQASAQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            46028..46342
                     /gene="yjcH"
                     /locus_tag="ACHJ69_000046"
     CDS             46028..46342
                     /gene="yjcH"
                     /locus_tag="ACHJ69_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO5013133.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            46339..47988
                     /gene="actP"
                     /locus_tag="ACHJ69_000047"
     CDS             46339..47988
                     /gene="actP"
                     /locus_tag="ACHJ69_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO5013134.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKCVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(48027..48716)
                     /locus_tag="ACHJ69_000048"
     CDS             complement(48027..48716)
                     /locus_tag="ACHJ69_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFO5013135.1"
                     /translation="MANFQLSMLCLAITLVIYFLNKRLYRRFRKLPLMPLVLTPTLLV
                     MLLVFGNISWQNYIGEAHWLLWLLGPATIAFAVPVYDNRAIIKRHWMSLTAGVLTATV
                     VAVTSSVWLARLFTLPDEIQRSLAVRSVTTPFALAASQSLGGQPDLVALFVVVTGVFG
                     MAIGDVLFLRLSIREGMAKGAGFGAASHGAGTARSYELGQQEGVVASLVMMLSGVLMV
                     LVSPLVARMMF"
     gene            complement(48709..49119)
                     /locus_tag="ACHJ69_000049"
     CDS             complement(48709..49119)
                     /locus_tag="ACHJ69_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO5013136.1"
                     /translation="MPVALRRITPAVVQSIQVFFQVVLYAGLFIFAQYLVSWLHLPLP
                     ANLVGMILMLALIVCRILPLQWVRAGARWLLAEMLLFFVPAVVAVVNYAQLLLVDGWR
                     IFAVIALSTVMVLGTTAWVVEKVYRYEMRRLNRG"
     gene            49223..50107
                     /locus_tag="ACHJ69_000050"
     CDS             49223..50107
                     /locus_tag="ACHJ69_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5013137.1"
                     /translation="MDIRTLRYFVEVVRQQSFTRAAEKLFVTQPTISKMLKNLEDELN
                     CILLIRDGRRLLLTDTGRVVFERGLALLAEFRQLEAELSDINQLKTGVLRLGIPPMVG
                     MLMAGPIGLFRQRYPGVELKISEFGGLTVQQAVMNGELDMALTALPVEESGLTTLSLF
                     SHPLCVLTPHSGKWLECDAVSPETLAEHPLLIYNEDFALSKQLMKLFDEHGVKPRIAV
                     RSGQWDFLAAMVQAGVGIAILPEPICQRLDKNTLKWLPLESELSWKLGMIWREGVYLS
                     HSAHAWLSCCEGYWLTPE"
     gene            complement(50143..51792)
                     /gene="yjcE"
                     /locus_tag="ACHJ69_000051"
     CDS             complement(50143..51792)
                     /gene="yjcE"
                     /locus_tag="ACHJ69_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO5013138.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSILEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(51943..53292)
                     /gene="ghxP"
                     /locus_tag="ACHJ69_000052"
                     /gene_synonym="yjcD"
     CDS             complement(51943..53292)
                     /gene="ghxP"
                     /locus_tag="ACHJ69_000052"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO5013139.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFTTFPVMMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(53839..54303)
                     /gene="soxR"
                     /locus_tag="ACHJ69_000053"
     CDS             complement(53839..54303)
                     /gene="soxR"
                     /locus_tag="ACHJ69_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO5013140.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            54389..54712
                     /gene="soxS"
                     /locus_tag="ACHJ69_000054"
     CDS             54389..54712
                     /gene="soxS"
                     /locus_tag="ACHJ69_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO5013141.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(54748..56301)
                     /gene="pdeC"
                     /locus_tag="ACHJ69_000055"
     CDS             complement(54748..56301)
                     /gene="pdeC"
                     /locus_tag="ACHJ69_000055"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO5013142.1"
                     /translation="MSHRARHQLLAFPGIIFLVLFPIILSLWIAFFWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIASVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHTDRYVSINLSASDFHTSRLIARTNQKTEQHAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRKAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEAQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMG"
     gene            56731..57012
                     /gene="yjcB"
                     /locus_tag="ACHJ69_000056"
     CDS             56731..57012
                     /gene="yjcB"
                     /locus_tag="ACHJ69_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO5013143.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRADYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(57111..57647)
                     /gene="ssb1"
                     /locus_tag="ACHJ69_000057"
     CDS             complement(57111..57647)
                     /gene="ssb1"
                     /locus_tag="ACHJ69_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO5013144.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            57902..60724
                     /gene="uvrA"
                     /locus_tag="ACHJ69_000058"
     CDS             57902..60724
                     /gene="uvrA"
                     /locus_tag="ACHJ69_000058"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO5013145.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(60933..61289)
                     /gene="yjbR"
                     /locus_tag="ACHJ69_000059"
     CDS             complement(60933..61289)
                     /gene="yjbR"
                     /locus_tag="ACHJ69_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO5013146.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(61293..61709)
                     /gene="yjbQ"
                     /locus_tag="ACHJ69_000060"
     CDS             complement(61293..61709)
                     /gene="yjbQ"
                     /locus_tag="ACHJ69_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO5013147.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRAVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(61820..62533)
                     /gene="aphA"
                     /locus_tag="ACHJ69_000061"
     CDS             complement(61820..62533)
                     /gene="aphA"
                     /locus_tag="ACHJ69_000061"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO5013148.1"
                     /translation="MRKITQALSAVCLLFALNSSAVALASSPSPLNPGTNVAKLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKLGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            62810..64630
                     /locus_tag="ACHJ69_000062"
     CDS             62810..64630
                     /locus_tag="ACHJ69_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5013149.1"
                     /translation="MKISWNYIFKNKWRFHITSISLFLIMLAVSIAFLHLRFNTLSGT
                     DKMRLEMYKSTLYSTIEQFYVLPYMLSTDHIIRQAVITPDDMTSSELNQRIAHFNTQL
                     KTAAIFILDTQGKAIASSNWQDPGSYVGQNYSYRPYYKHAMSGLNGRFYGIGSTTNTP
                     GFFLSTSIKDKGKIVGVVVVKISLNEIEKAWAEGPENIIVNDEHGIIFLSSKSPWRMR
                     TLQPLPVQAKQKLQSTRQYSLDNLLPADYYPCYTVSNFTFLKDKKEQLCLFPQYYTQQ
                     IAIPEFNWKMTIMVPLDNLYWSWAISLVITLIIYLLFLLFIKYWRMRSHAQQLLTLAN
                     ETLEKQVKERTSALELINQKLIQEIKERSQAEQVLQITRSELAESSKLAALGQMATEI
                     AHEQNQPLAAIHALTDNARTMLKKEMYPQVEQNLKHIISVIERMTQLISELKAFASRH
                     RVPKGSADVIKVMYSAVALLNHSMEKNNIERRIKAPSMPLFVNCDELGLEQIFSNLIS
                     NALDSMEGSSYKRLDIAIRQANNKVIITIKDSGGGFAPEVVDRIFEPFFTTKRRGMGL
                     GLAIVSEIVRNSNGALHASNHPEGGAVMTLTWPEWGEEHE"
     gene            64623..65981
                     /locus_tag="ACHJ69_000063"
     CDS             64623..65981
                     /locus_tag="ACHJ69_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFO5013150.1"
                     /translation="MNNKSSEITIVYIEDSDDVRFACEQTLTLAGYRVISCCDAEHSI
                     PLIQSQANIIILTDVRLPGISGLELLSYINEMDSKIPVILITGHGDVEMAVDAMRNGA
                     FDFIEKPSSSDKLLSIIARAVEKRRLVLENQQLLANLQQENGPVLIGRSPQMQQLRKM
                     ILNVADTGADVLIYGETGCGKEVVARMLHHWSTRRQGQFVALNCAGLPETLFESEIFG
                     HEAGAFTGAVKKRIGKIEHANGGTLFLDEIEGMPSGMQVKLLRVLQERTIERLGANQL
                     IPVNCRVIAATKEDLLRRSEEHLFRLDLYYRLNVVSLNIPPLRQRREDIPELFYWFAS
                     QAAQKYNRPLPDISPMLLAWLQSQSWPGNVRELKHNAERFVLGLLTHHQPVPMTQQEE
                     SGLTACIDAFEKKLIEDMLRQTEGQVSLTARLLQLPRKTLYDKLNKHQIQPQVYRPES
                     SS"
     gene            complement(65970..67043)
                     /locus_tag="ACHJ69_000064"
     CDS             complement(65970..67043)
                     /locus_tag="ACHJ69_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HFO5013151.1"
                     /translation="MSLQIAVEKVRWLAAGLLELNGCDADIAQDVAEHMIEAERYGFA
                     SHGVTLLPKYLENIARGDVTANARPECLTSEGNLQRFHAHNGFGQHAGKVAVNAAIEQ
                     AKHRGFCLLTLCHAHHLGRMGHYGQMVADQGLAMLAMSNVTGRPALVAPWGGAEPRMT
                     TNPFCFAWPFSDGRPPILVDFATSSMALNKARVMSSTGKQAAPGQLIDAQGNPSNDPG
                     VLFSNPPGALLPFGEHKGFGLALMIEMMAGILSGGDTIAEDHQTDGSAHNHLFALIID
                     PDQFTDLAGEKGQFFADYLLATQPQPGGNPVIYPGMPEAANRERNAKMITIPVSFWSW
                     VVEHYARKGIDLGLHPQESALAG"
     gene            complement(67055..68554)
                     /locus_tag="ACHJ69_000065"
     CDS             complement(67055..68554)
                     /locus_tag="ACHJ69_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFO5013152.1"
                     /translation="MSSISHGAPQKRRIIPNPGLWLAIIAGIIITLLPLGDTLPVAGQ
                     NMIAILVFAIIVWISEAMDYTASAIVISALIIFMVGFAPDMNHPDTILGTAKALKMTL
                     SGFSNSALALVAAAMFIAAAMTITGLDKRIALFTMSKIGASSRSIIIGAIVVTIVLSL
                     VVPSATARTACVVPIMMGVIAAFKVDKHSRLAASMMIVIAQATSIWNVGIQTSAAQNL
                     LSIGFINKTFGAGHSVSWLDWLLAGAPWSLTMSAILYFLARKLLPPETEAVEGGSEAI
                     KKALAELGPTTGKEKRLIGISLLLLLFWSTGGKLHSIDTTSVTLAGLAIMLLPGIGVM
                     SWKEVEKRVQWGTLLMFGIGISLGSTLLDTQAASWMANYVVKGFGLDGLPSLAIFAIL
                     AAFLIIIHLGFASATALTAALLPILISLLSSLPPELGVNPVGMTILLAFSVSFGFILP
                     INAPQNMVCMGTDTFTPRQFTRVGLYLTVIGYLLLLLFAATWWKILGLM"
     gene            complement(68766..69638)
                     /gene="sucD"
                     /locus_tag="ACHJ69_000066"
     CDS             complement(68766..69638)
                     /gene="sucD"
                     /locus_tag="ACHJ69_000066"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO5013153.1"
                     /translation="MSVLINKHTRVLVQGITGKNGTFHTEQAIAYGTQIVGGVTPGKG
                     GQRHLDRPVFDSMKEAMRQTEADASVIYVPAPFVLDSVVEAIEAGVKLVVVITEGVPT
                     LDMVKVRRLLDCHPDVRLIGPNCPGVITPGECKIGIMPGEIHRPGRIGIVSRSGTLTY
                     EAVAQTTALGLGQSTCIGIGGDPVPGTNFIDALRLFENDPQTDAVIMIGEIGGNAEER
                     AAEFIRDEMNKPVVGYIAGVTAPKGKRMGHAGAIISGGSGSAEDKFRAFDKAGMAWTR
                     NPALLGETLWNVIK"
     gene            complement(69651..70820)
                     /gene="sucC"
                     /locus_tag="ACHJ69_000067"
     CDS             complement(69651..70820)
                     /gene="sucC"
                     /locus_tag="ACHJ69_000067"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO5013154.1"
                     /translation="MNLHEYQAKSLLAGMGMPCPKEIAIQQISQLADAWQHIACPSKG
                     AVLKAQVHAGGRGKAGGVKVLKQLPEAQAFVQQMLGSQLVTYQTGPEGQYVSSILLCE
                     NIYPVRQELYFGMVVDRESQRVTFIVSPEGGVEIEKVAHETPEKISSVSIDPLTGVQP
                     CHIREMFAVLQLEHGLFAAFSRLVNQAWKAFNELDFALLEINPLVLRETGEFMCADAK
                     VSLDDNALYRHPELQVLRDETQEDPRESQAAKLDLNYVSLDGNIGCMVNGAGLAMATM
                     DIIKLYGEQPANFLDVGGGATQERVSEAFRLIVSDSKVKAILVNIFGGIVRCDMIARA
                     IIHALNEARITLPVVVRLSGNNAAEGQRLLAESGLTVEAVNSLDDAAKRIIALLN"
     gene            complement(70842..72260)
                     /gene="lpdA"
                     /locus_tag="ACHJ69_000068"
     CDS             complement(70842..72260)
                     /gene="lpdA"
                     /locus_tag="ACHJ69_000068"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO5013155.1"
                     /translation="MSTIFDVAVMGGGPGGYVAALRAAQNGLSVVCIDDGVNAQGEPS
                     PGGTCLNVGCIPSKSLLQSSELYAQVQHEASIHGVNVDGVSFNAAAMIQRKDAIVSRL
                     TMGIGLLFKKNKVKYLCGLATLEHARDEIWQLRVNDQQIRARNVVIATGSQPRQLPGV
                     TIDNQQILDNRGALALTAVPPRLGVIGAGVIGLELGSVWNRVGSDVTLLEMAPTFLPA
                     LEARLSNEVRKAMIASGMKMQLAVEIEAIEQRDDGVHVRWRQGEKREESRFDKLILAI
                     GRVPRLSGVDLVQLGLEADNRGGIAVDNLCRTGKAGLWAIGDVVRGPMLAHKAMAEGV
                     VVADQIAGLAVEPINFALIPSVIYTQPEVAWVGENEASLKAAGRVFNKGNSLFAGNGR
                     ALALGQEGGRCTLYSDKHTDRVLGGAIVGPQASELINEIALAMTFSASGEDIACAIHA
                     HPTLSEVIHEAAMALNNKALHG"
     gene            complement(72272..73418)
                     /gene="sucB"
                     /locus_tag="ACHJ69_000069"
                     /pseudo
     CDS             complement(72272..73418)
                     /gene="sucB"
                     /locus_tag="ACHJ69_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468318.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
     gene            complement(73451..76195)
                     /locus_tag="ACHJ69_000070"
     CDS             complement(73451..76195)
                     /locus_tag="ACHJ69_000070"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO5013156.1"
                     /translation="MENMTSPGTLLSGDNATWLEDYYQTWLRTPEQLPEDWRRFFLSP
                     ELTVQSVSGDNNVSGATLKKQAAIIQLINAWRTQGHLRAKLDPLGLNPPADVPSLLPG
                     FWGLSEEDLLQEFSVTFGAHTTQMPLKQLLNLLEQAWAGSQAYELAHLENREEINWLL
                     SRIESSNAPQADVQTCIARFEKLMAAETLERYLHTRYVGQKRFSLEGGESAIPALDTL
                     TKRLRAQGVEEMVIGMAHRGRLNVLVNLLNKDPAQLFAEFEGKQTIGSGSGDVKYHMG
                     YSSNLETPAGSLHVALAYNPSHLEIVNPVVLGQVRARQERRGEDGQAKVVGVLIHGDS
                     ALGGLGVNQTTFNLSQTQGYGTGGTLHLVINNQIGFTTSRLQDMRSSRYCTDIAKMVA
                     APIIHVNGDDVDAVCQVMELACEWRDTFRRDIIIDICCFRKHGHNESDEPRLTQPQMY
                     QAVDAHPGTLARYGESLARRGLLTQAQQDEMTARYRDWLDSCQKREPQPLKPAIHSFS
                     ANWYGLTNPHWSAPVSTALPRQKLAAYGEIISTLPPDVVAHPTIKRQLALRQDMAAGT
                     QPIDWGMAEMLAYASLVDAGVGVRLSGEDSGRGTFSHRHAVVHHQTEARRYLPLQHIR
                     AGQASFDVYDSVLNEEALLAFEYGYSTSAPQQLVIWEAQFGDFANGAQVAIDQFISSG
                     ETKWDRYSGLTILLPHGYDGQGPEHSSARPERWLQLCAENNMQVVMPSESAQMFHLLR
                     GQALRPMRKPLVIMMSKRLLRFKGAMSELCEFTDGAYKPVITDPQLHQSQKVKRVILC
                     SGQVYYDVLEARKQREHEDEVAIVRLEQLYPFPVAELNDVLASWPNCCEWIWLQEEPE
                     NQGAWRQIRHELAALKINTPYWQYAGRPAAAAPATGYGRVHKQQIDEFLAAAFADIQP
                     "
     gene            complement(76377..77570)
                     /gene="tyrB"
                     /locus_tag="ACHJ69_000071"
     CDS             complement(76377..77570)
                     /gene="tyrB"
                     /locus_tag="ACHJ69_000071"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO5013157.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYNEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDSVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAKVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLRTAQVDRLREEFGVYLIASGRMCVAGLNAQNVHRVAKA
                     FAAVM"
     gene            complement(77823..78902)
                     /gene="alr"
                     /locus_tag="ACHJ69_000072"
     CDS             complement(77823..78902)
                     /gene="alr"
                     /locus_tag="ACHJ69_000072"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO5013158.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(78955..80370)
                     /gene="dnaB"
                     /locus_tag="ACHJ69_000073"
     CDS             complement(78955..80370)
                     /gene="dnaB"
                     /locus_tag="ACHJ69_000073"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO5013159.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            80453..81436
                     /gene="qorA"
                     /locus_tag="ACHJ69_000074"
     CDS             80453..81436
                     /gene="qorA"
                     /locus_tag="ACHJ69_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO5013160.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDARRAHEILESRATQGSSLLIP"
     gene            complement(81601..81843)
                     /gene="pspG"
                     /locus_tag="ACHJ69_000075"
     CDS             complement(81601..81843)
                     /gene="pspG"
                     /locus_tag="ACHJ69_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO5013161.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALIVATAFMFLGGMLALM
                     IKLLPWLLLAVAVVWVIRAIKAPKVPKYQRYDRWRY"
     gene            complement(81977..82972)
                     /gene="dusA"
                     /locus_tag="ACHJ69_000076"
     CDS             complement(81977..82972)
                     /gene="dusA"
                     /locus_tag="ACHJ69_000076"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO5013162.1"
                     /translation="MPAESHTVYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEM
                     VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPS
                     DRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
                     SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSL
                     EEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIEREL
                     SQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR"
     gene            83128..83643
                     /gene="zur"
                     /locus_tag="ACHJ69_000077"
     CDS             83128..83643
                     /gene="zur"
                     /locus_tag="ACHJ69_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO5013163.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALEFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCSACVEVEACRHPEQCHH
                     DHSIQVKKKPR"
     gene            complement(83685..83894)
                     /gene="yjbJ"
                     /locus_tag="ACHJ69_000078"
     CDS             complement(83685..83894)
                     /gene="yjbJ"
                     /locus_tag="ACHJ69_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO5013164.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVDSWEKRHDYRW"
     gene            complement(84010..85335)
                     /gene="dinF"
                     /locus_tag="ACHJ69_000079"
     CDS             complement(84010..85335)
                     /gene="dinF"
                     /locus_tag="ACHJ69_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO5013165.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRHLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNDTWFVAT"
     gene            complement(85408..86016)
                     /gene="lexA"
                     /locus_tag="ACHJ69_000080"
     CDS             complement(85408..86016)
                     /gene="lexA"
                     /locus_tag="ACHJ69_000080"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO5013166.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(86126..86494)
                     /gene="dgkA"
                     /locus_tag="ACHJ69_000081"
     CDS             complement(86126..86494)
                     /gene="dgkA"
                     /locus_tag="ACHJ69_000081"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO5013167.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            86665..89088
                     /gene="plsB"
                     /locus_tag="ACHJ69_000082"
     CDS             86665..89088
                     /gene="plsB"
                     /locus_tag="ACHJ69_000082"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO5013168.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(89243..90115)
                     /gene="ubiA"
                     /locus_tag="ACHJ69_000083"
     CDS             complement(89243..90115)
                     /gene="ubiA"
                     /locus_tag="ACHJ69_000083"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO5013169.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(90128..90625)
                     /gene="ubiC"
                     /locus_tag="ACHJ69_000084"
     CDS             complement(90128..90625)
                     /gene="ubiC"
                     /locus_tag="ACHJ69_000084"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO5013170.1"
                     /translation="MSHPALTQLRALRYFTEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(90804..91724)
                     /gene="malM"
                     /locus_tag="ACHJ69_000085"
     CDS             complement(90804..91724)
                     /gene="malM"
                     /locus_tag="ACHJ69_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO5013171.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLSTGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(92138..93478)
                     /gene="lamB"
                     /locus_tag="ACHJ69_000086"
     CDS             complement(92138..93478)
                     /gene="lamB"
                     /locus_tag="ACHJ69_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO5013172.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGSSSTNPYYGKAVSADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(93550..94665)
                     /gene="malK"
                     /locus_tag="ACHJ69_000087"
     CDS             complement(93550..94665)
                     /gene="malK"
                     /locus_tag="ACHJ69_000087"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO5013173.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLEMITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            95030..96220
                     /gene="malE"
                     /locus_tag="ACHJ69_000088"
     CDS             95030..96220
                     /gene="malE"
                     /locus_tag="ACHJ69_000088"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO5013174.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYQ
                     DELAKDPRIAATMDNAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDAALKDAQT
                     RITK"
     gene            96374..97918
                     /gene="malF"
                     /locus_tag="ACHJ69_000089"
     CDS             96374..97918
                     /gene="malF"
                     /locus_tag="ACHJ69_000089"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO5013175.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKIYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ESATQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            97933..98823
                     /gene="malG"
                     /locus_tag="ACHJ69_000090"
     CDS             97933..98823
                     /gene="malG"
                     /locus_tag="ACHJ69_000090"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO5013176.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(98917..99327)
                     /gene="psiE"
                     /locus_tag="ACHJ69_000091"
     CDS             complement(98917..99327)
                     /gene="psiE"
                     /locus_tag="ACHJ69_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO5013177.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFVPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            99541..99819
                     /locus_tag="ACHJ69_000092"
     CDS             99541..99819
                     /locus_tag="ACHJ69_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013178.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGLLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGY"
     gene            complement(99866..101962)
                     /gene="yjbH"
                     /locus_tag="ACHJ69_000093"
     CDS             complement(99866..101962)
                     /gene="yjbH"
                     /locus_tag="ACHJ69_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO5013179.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPKYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLGGEP
                     LGHETTLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGVGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(101962..102699)
                     /gene="yjbG"
                     /locus_tag="ACHJ69_000094"
     CDS             complement(101962..102699)
                     /gene="yjbG"
                     /locus_tag="ACHJ69_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO5013180.1"
                     /translation="MIKQTIVALLLSAGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGTPPSTVTLFGLISRPGKQS
                     FTLGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(102696..103333)
                     /gene="yjbF"
                     /locus_tag="ACHJ69_000095"
                     /pseudo
     CDS             complement(102696..103333)
                     /gene="yjbF"
                     /locus_tag="ACHJ69_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
     gene            complement(103520..103762)
                     /gene="yjbE"
                     /locus_tag="ACHJ69_000096"
     CDS             complement(103520..103762)
                     /gene="yjbE"
                     /locus_tag="ACHJ69_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO5013181.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(104260..105909)
                     /gene="pgi"
                     /locus_tag="ACHJ69_000097"
     CDS             complement(104260..105909)
                     /gene="pgi"
                     /locus_tag="ACHJ69_000097"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO5013182.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGKVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      106146..106342
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            106434..107783
                     /gene="lysC"
                     /locus_tag="ACHJ69_000098"
     CDS             106434..107783
                     /gene="lysC"
                     /locus_tag="ACHJ69_000098"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO5013183.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            108036..108308
                     /gene="yjbD"
                     /locus_tag="ACHJ69_000099"
     CDS             108036..108308
                     /gene="yjbD"
                     /locus_tag="ACHJ69_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO5013184.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(108309..109181)
                     /gene="rluF"
                     /locus_tag="ACHJ69_000100"
     CDS             complement(108309..109181)
                     /gene="rluF"
                     /locus_tag="ACHJ69_000100"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO5013185.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQCNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPRESEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            109529..110476
                     /locus_tag="ACHJ69_000101"
     CDS             109529..110476
                     /locus_tag="ACHJ69_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO5013186.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQVKQKFGLKDVVVVSGNDEDEDTQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDIFSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            110546..111352
                     /locus_tag="ACHJ69_000102"
     CDS             110546..111352
                     /locus_tag="ACHJ69_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5013187.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVDELLAQGANVQMVDIHCG
                     DGQYESHKGYQFWPTDISSAKEINHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            111362..111769
                     /locus_tag="ACHJ69_000103"
     CDS             111362..111769
                     /locus_tag="ACHJ69_000103"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO5013188.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLDKLVSTHNHDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNIDELCEHLTQIAKQTCVVWRQVATAEEDF"
     gene            111769..112263
                     /locus_tag="ACHJ69_000104"
     CDS             111769..112263
                     /locus_tag="ACHJ69_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO5013189.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            112329..113126
                     /locus_tag="ACHJ69_000105"
     CDS             112329..113126
                     /locus_tag="ACHJ69_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFO5013190.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIMLGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPHPQTTTITALDQLDD"
     gene            113137..113961
                     /locus_tag="ACHJ69_000106"
     CDS             113137..113961
                     /locus_tag="ACHJ69_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO5013191.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNLLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTPAADGAT
                     VTITVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            114013..115242
                     /gene="sorE"
                     /locus_tag="ACHJ69_000107"
     CDS             114013..115242
                     /gene="sorE"
                     /locus_tag="ACHJ69_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO5013192.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEHDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAIGGGKKLVYTGKYLPLTSLTQIQDQALAVILAHHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            115623..116312
                     /gene="pepE"
                     /locus_tag="ACHJ69_000108"
     CDS             115623..116312
                     /gene="pepE"
                     /locus_tag="ACHJ69_000108"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO5013193.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(116402..118033)
                     /gene="yjbB"
                     /locus_tag="ACHJ69_000109"
     CDS             complement(116402..118033)
                     /gene="yjbB"
                     /locus_tag="ACHJ69_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO5013194.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLPLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(118204..118653)
                     /locus_tag="ACHJ69_000110"
     CDS             complement(118204..118653)
                     /locus_tag="ACHJ69_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013195.1"
                     /translation="MQRQYKKYILLFWGVMDVIAIATYLFYSLGNGRIPFYSDIVHSL
                     SLLKDIGVEGGFYAYAVATVTLQIVLLFSLFFSAQCFLRQKLFSFSFLAIQEFMRFAT
                     VSCSISVIPLLLNYFYSQDLVLNISLFIFSEVTKVTTIIWCKRQGKV"
     gene            complement(118696..119016)
                     /locus_tag="ACHJ69_000111"
     CDS             complement(118696..119016)
                     /locus_tag="ACHJ69_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013196.1"
                     /translation="MIKIDEIDFFAFEGCSESEVTELLGKKPTGYNDWIINIKTGVEW
                     VFYDGRYWLDFRKSKEGIRCISCSILYHDKSKGRIDVLFSEGIADRGLKNTGGVLIQK
                     RPQL"
     gene            complement(119076..122759)
                     /gene="metH"
                     /locus_tag="ACHJ69_000112"
     CDS             complement(119076..122759)
                     /gene="metH"
                     /locus_tag="ACHJ69_000112"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO5013197.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPAEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPTELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANTQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMEHQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKMLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAE"
     gene            122959..123783
                     /gene="iclR"
                     /locus_tag="ACHJ69_000113"
     CDS             122959..123783
                     /gene="iclR"
                     /locus_tag="ACHJ69_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO5013198.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGIR"
     gene            complement(123800..125524)
                     /gene="aceK"
                     /locus_tag="ACHJ69_000114"
     CDS             complement(123800..125524)
                     /gene="aceK"
                     /locus_tag="ACHJ69_000114"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO5013199.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKGHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDRFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPLPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRFV"
     gene            complement(125740..127044)
                     /gene="aceA"
                     /locus_tag="ACHJ69_000115"
     CDS             complement(125740..127044)
                     /gene="aceA"
                     /locus_tag="ACHJ69_000115"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO5013200.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(127074..128675)
                     /gene="aceB"
                     /locus_tag="ACHJ69_000116"
     CDS             complement(127074..128675)
                     /gene="aceB"
                     /locus_tag="ACHJ69_000116"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO5013201.1"
                     /translation="MTEQATTIDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYSNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGETIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLDKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSHGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(128944..129873)
                     /gene="metA"
                     /locus_tag="ACHJ69_000117"
     CDS             complement(128944..129873)
                     /gene="metA"
                     /locus_tag="ACHJ69_000117"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO5013202.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            130030..>130289
                     /locus_tag="ACHJ69_000118"
     CDS             130030..>130289
                     /locus_tag="ACHJ69_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO5013203.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVH
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGV"
BASE COUNT        31796 a        34873 c        33262 g        30358 t
ORIGIN      
        1 tttctttttg cgtttccttt caaatgaaaa cgatcgtcgt ctaaaatcag cagtaccccc
       61 gacaaactca gggattttgt gtataattgc ggcctttttc ggcaatctgc cgttttttgg
      121 cgcttttgcc ctgctgactt ttgaggaaat ccacatgtca ttaccacact gcccaaaatg
      181 caactccgaa tacacctacg aagataacgg catgtacatc tgcccggaat gtgcctacga
      241 atggaacgac gcagaacctg cacaggaaag cgacgagctg atcgttaaag atgctaacgg
      301 caatctgctg gctgacggcg acagcgtgac catcattaaa gatctgaagg tgaaaggtag
      361 ctcttcgatg ctgaaaattg gcaccaaagt gaaaaacatc cgcctggttg aaggcgacca
      421 taacatcgat tgcaaaatcg acggttttgg tccgatgaaa ctgaaatctg agtttgtgaa
      481 aaagaactga ttgtattgtg atcggtaagc cggataaggc gctcgcgccg catccggcaa
      541 cgatgccgac tgcctgatgc gacgctgacc gcgtcttatc aggcttccct ctcatctgta
      601 taaatttcga actacactta actggcttct cttaactgag gtcaccatca tgccgttaag
      661 tccctacctc tcttttgccg gtaactgttc cgacgcgatt gcctattatc aacgtacgtt
      721 gggcgcggaa ctgctctata aaatcagctt cggcgaaatg ccgaaatcag cgcaggacag
      781 cgccgagaac tgcccttccg gaatgcaatt tcccgatacc gccatcgctc atgccaacgt
      841 gcgcattgca ggaagcgaca tcatgatgag cgatgctata ccgtcaggaa aagccagcta
      901 ctccggcttt acgctggtgc tcgattcgca acaggtcgaa gaaggaaaac gctggtttga
      961 caaccttgcc gctaacggaa aaatcgaaat ggcctggcag gaaactttct gggcgcatgg
     1021 cttcggcaaa gtcaccgata aatttggcgt accgtggatg attaatgtcg tcaaacaaca
     1081 accaacgcac taacccgccg ggagggccgc cctcccgcac tgtcatcgaa atctcgttaa
     1141 ctcttcatcc gttagtcact tttagttaac aaaatcgtca caataatccg ccacgatgga
     1201 gccacttttt tagggaggct gcatcatgca aacgattatc cgtgtcgaga agctcgccaa
     1261 aaccttcaat cagcatcagg cgctgcacgc ggttgatctg aacattcatc atggtgaaat
     1321 ggtggctctg cttgggccgt cgggttccgg aaaatccact cttttacgtc acttaagcgg
     1381 tttgattacc ggcgataaat ctgtcggtag ccatatcgag ctgctgggcc gcactgtcca
     1441 gcgcgaaggc cgtctggccc gcgatatccg caaaagccgc gcccataccg gctacatctt
     1501 tcaacagttc aacctggtga atcgcctgag cgtactggag aacgtgctga ttggcgcgct
     1561 cggcagcacg ccattctggc gaacctgttt tagctacttc acccgcgagc agaaacagcg
     1621 cgcgttacag gcgctgaccc gcgttggcat ggtgcacttt gcccatcaac gtgtttccac
     1681 actctccggc ggccagcaac agcgtgtggc gattgcccgc gcgctgatgc agcaggcgaa
     1741 ggtcattctc gctgacgaac ccatcgcctc gctggacccg gaatcggcac gcatcgtgat
     1801 ggacaccctg cgcgacatca accagaacga cggcattacc gtggtcgtca cgctgcatca
     1861 ggtggattac gccctgcgct actgcgaacg catcgtcgcc ctgcgccagg ggcacgtctt
     1921 ctacgacggc tgcagccaac agtttgataa cgaacgtttt gaccatctct accgcagcat
     1981 taaccgcgtc gaagagaacg cgaaagctgc ctgacatccc catcattgag gaaaacgaat
     2041 gaacgctaag ataattgcct cgctggcctt caccagcatg ttcagcctca gcaccctgtt
     2101 aaatccggca tacgccgaag agcaggaaaa ggcgctgaat ttcggcatta tttcaacgga
     2161 atcacagcaa aacctgaaac cgcaatggac gccgttctta caggatatgg agaagaagct
     2221 gggcgtaaag gtcaacgcct tctttgcccc ggactacgcg ggcattatcc aggggatgcg
     2281 cttcaataaa gtggatatcg cctggtacgg caacctgtcg gcgatggaag cggtggatcg
     2341 cgccaacggt caggtcttcg cccagacggt cgcggcggat ggatcgccgg gttactggag
     2401 cgtgttgatc gtcaacaaag atagcccgat caacaacctg aacgatctgc tggcgaagcg
     2461 gaaagatctc accttcggca atggcgatcc taactctacc tccggcttcc tcgtccccgg
     2521 ttactacgtc ttcgctaaaa acaatatctc cgccagcgac tttaagcgca ccgtcaacgc
     2581 cgggcatgaa accaacgccc tcgccgtcgc taacaagcaa gtggatgtcg ccaccaacaa
     2641 caccgaaaac ctcgacaagc tgaaaacctc cgcgccggag aagctgaaag aactgaaggt
     2701 gatctggaaa tcaccgctga tccctggcga tccgatcgtc tggcgcaaaa atctttccga
     2761 aaccactaaa gacaagatct acgacttctt tatgaattac ggcaaaacgc cggaagagaa
     2821 agcggtgctg gagcgcctgg gctgggcgcc gttccgcgcc tccagcgact tgcaactggt
     2881 gccgattcgc cagctcgcac tgtttaaaga gatgcaaagc gtgaaggaca acaaagggct
     2941 gaacgaacag gacaagctgg caaaaaccac cgccattcag gcgcaactgg atgatttgga
     3001 ccgcctgaac aacgcgttaa gcgcaatgag ttcggtgagt aaagcggtgc agtaaatcgt
     3061 aggtcggata agacgccccg gcgtcgcatc cgacaatgtg cagacgttga tgccggatgc
     3121 ggtgcaagca ccttatccgg cctacagacc ggagccaaac atgcaaacca tcaccatcgc
     3181 cccacccaag cgcagctggt tctcgcttct gagctgggcc gttgttatcg ccgtgctgat
     3241 cgtctcgtgg cagggcgcgg aaatggcccc gcttacgctg attaaagatg gcggcaacat
     3301 ggcgacgttc gccgccgact tcttcccgcc cgatttcagc cagtggcagg attacctcac
     3361 cgaaatggcc gtcacgctgc aaatcgccgt ctggggcacc gcgctggcgg tggttctctc
     3421 catccccttc ggcctgatga gcgctgaaaa cctggtaccg tggtgggtct accaacctgt
     3481 acgccgcctg atggacgcct gccgcgccat taacgaaatg gtcttcgcca tgctgttcgt
     3541 ggtcgccgtc ggcctcggcc cgttcgctgg cgtgctggcg ctgtttatcc acaccaccgg
     3601 cgtactttcc aaactgcttt ccgaagcggt agaagcgatt gaacccggcc cggtggaggg
     3661 cattcgcgct accggagcca acaagctgga agagatcctc tacggcgtac taccgcaggt
     3721 catgccgctg ctgatttcct actccctcta tcgcttcgaa tccaacgtcc gctcggcgac
     3781 cgtcgtcggt atggttggcg cgggcgggat cggcgtcacg ctgtgggaag cgattcgcgg
     3841 tttccagttc cagcaaacct gcgccctgat ggtgctcatc atcgtcacgg tcagcctgct
     3901 ggatttcctc tctcaacggt tgcgtaagca ctttatctga taagcgaggc attgatatct
     3961 atgcacttgt ctacacatcc gaccagctac ccaacacgct atcaggagat agccgcaaaa
     4021 cttgagcagg agcttcgtca acactaccgc tgcggcgact atcttcccgc cgagcagcaa
     4081 ctggcggcgc gctttgaggt gaaccgccac accctgcgcc gcgccatcga ccaactggtg
     4141 gaaaaaggct gggtacagcg ccgtcagggc gtcggcgtac tggtgctgat gcgcccgttc
     4201 gattacccgc tcaacgccca ggcgcgtttt agccagaacc tgctggacca gggcagccat
     4261 cccaccagcg aaaaattgct atcggtgctg cgccccgcct ccggtcacgt cgctgacgcg
     4321 ctggggataa ccgaggggga gaacgtcatc cacctgcgca ccctgcgtcg ggtcaacggc
     4381 gttgcgctct gtttaatcga ccactacttc gcagatctca cgctctggcc aacgcttcaa
     4441 tgctttgaca gcggttcgct gcacgatttt ctgcgcgagc aaacggggat tacgctacgc
     4501 cgcagccaga cgcggatcag cgcccgccgc gcccaggcca aagagtgcca gcatcttgaa
     4561 atcccgaata tgtcgccgct gctgtgcgtg cgcaccctta accaccgtga cggtgaaagc
     4621 agcccggcgg agtactccgt cagcctgacg cgcgccgaca tgattgaatt cactatggag
     4681 cactgaatgc acgcagatac cgcgacccgc cagcactgga tgtccgtgct ggcgcacagc
     4741 caaccggctg aactggcagc ccgcctgaac gcgctgaaca tcaacgccga ttatgaggtg
     4801 atccgcgccg ctgaaactgg cctggtgcag attcaggcgc gaatgggcgg caccggcgaa
     4861 cgcttttttg ctggcgacgc cacgctcacc cgcgccgccg tgcgtctgac tgacggcacg
     4921 ctcggctata gctgggtgct ggggcgcgac aaacagcacg ccgaacgttg tgcgctgatt
     4981 gatgcgctga tgcagcaaaa ccgccacttt caaaacttat cagaaaccct tattaccccg
     5041 ctggacgctg accgtatggc acgcattgcc gcgcgtcagg ccgaagtgaa cgccagccgg
     5101 gtcgacttct ttacgatggt tcgcggagac aacgcatgac cctggaaacc gcttttatgc
     5161 ttcccgtgca ggatgcccag cacagttttc gtcgcctgtt aaaggccatg agcgagccgg
     5221 gcgtgattgt cgccctgcat cagctcaagc gcggctggca gccgctgaat atcgccacca
     5281 ccagcgtgct gctgacgctg gccgataacg acacgccggt gtggcttgcc gcaccattga
     5341 gcaacgatat cgtcaatcag agcctgcgtt ttcataccaa cgcgccgctg gttaatcagc
     5401 cggaacaggc gaccttcgcg gtaacagatg aggcgatctc cagcgagcag ctcaacgccc
     5461 tttccaccgg cactgccgtt gcaccagaag cgggcgcaac gctgatttta caggtcacca
     5521 gcctgagcgg tgggcgtatg ttgcgcctca ccggcgcagg tattgccgaa gaacggatga
     5581 tcgccccgca actgccaaag tgcattcttc atgaacttac cgagcgcccg cacccgttcc
     5641 cgctcggcat cgacctgatc ctgacctgcg gcgagcgcct gctggctatt ccgcgaacca
     5701 ctcatgtgga ggtgtgctga tgtacgttgc cgtgaaaggg ggcgaaaagg cgatcgacgc
     5761 cgcccacgcc ctgcaagaga gccgacgccg gggcgatact gatttgcctg aactgagcgt
     5821 cgcccagatt gaacagcagc ttaacctcgc ggtagatcgc gtgatgaccg aaggcggtat
     5881 tgccgaccgc gagctggccg cgctggcgct gaaacaggcc agcggcgata acgtcgaagc
     5941 gatttttctg ttgcgcgcct accgcaccac gctggcgaag ctggcggtca gtgagccgct
     6001 cgacaccacc gggatgcgtc tcgaacggcg tatttcagca atttataaag acattcccgg
     6061 cggccaactg cttggcccca cctacgacta cacccatcgc ctgctcgatt tcaccctgct
     6121 ggcaaacggc gaaacgccaa cgctgaccac cgctgacagc gaacagcaac cgtcgccaca
     6181 cgtcttcagc ctgctggcgc gtcaggggct ggcgaagttt gaagaagata gcggcgcaca
     6241 accggacgac atcacccgca cgccgccggt ttatccctgt tcgcgttctt cccgtctaca
     6301 acaattaatg cgcggtgacg aaggctattt gttggcgctg gcctactcca cccaacgcgg
     6361 ttacgggcgc aatcacccgt tcgcaggcga aattcgcagt ggctatatcg atatatttat
     6421 tgtgccggaa gagctgggat ttgcggtgaa cgtcggcgaa ttgttgatga ccgagtgtga
     6481 aatggtcaac ggttttatcg acccgccgga tgaaccaccg cattttacac gcggctatgg
     6541 gctggtgttc ggcatgagcg agcgcaaagc aatggcgatg gcgctggttg accgtgcgtt
     6601 gcaggcaccg gaatacggcg agcacgcaac aggtccggca caggatgaag agttcgtgct
     6661 ggcccatgcc gacaacgtcg aagccgcagg ctttgtttcg catctcaaac tcccgcacta
     6721 cgtcgatttc caggccgaac tggagctact caaacgtctg caacaggagc agaaccatgg
     6781 ctaatctgag cggctacaac tttgcttacc tcgacgagca gaccaaacgc atgatccgcc
     6841 gcgctatctt aaaagcggtg gcaatccccg gttatcaggt gccgtttggc ggacgcgaga
     6901 tgccgatgcc ctacggctgg ggaaccggcg gcattcagct caccgccagc gtgattggcg
     6961 aaagcgacgt gttgaaggtg attgaccagg gcgcagacga caccaccaac gccgtgtcga
     7021 ttcgtaactt ctttaagcgc gtgaccgggg tgaacaccac cgagcgcacg gacgatgcga
     7081 cacttatcca gacgcgtcac cgcatacctg aaacgccgct gaccgaagat cagatagtta
     7141 tcttccaggt gccgatcccc gaaccgctgc gctttattga gccgcgtgag acggaaacgc
     7201 gcaccatgca cgcgctggag gagtacggcg tgatgcaggt gaaactgtat gaagatatcg
     7261 cccgcttcgg tcatatcgcc accacctacg cctatccggt gaaggtgaac gggcgctacg
     7321 taatggaccc gtcgccgatc ccgaaattcg ataacccgaa aatggacatg atgcccgccc
     7381 tgcaactgtt tggcgcgggg cgcgagaagc gcatctatgc cgtgccaccg tttacccgcg
     7441 tggaaagcct cgatttcgat gatcatccgt tcaccgtcca gcagtgggat gaaccatgcg
     7501 ccatctgcgg atcgacccac agttacctcg acgaagtggt gctggatgac gccggaaacc
     7561 gcatgtttgt ctgctccgat accgattatt gccgccaaca gaacgaggcc aaaagccaat
     7621 gaatcaaccg ttactttcgg tcaataacct gacccacctt tatgccccgg gcaaaggctt
     7681 cagcgatgtc tcttttgatt tatggccggg agaagttctg ggcattgttg gggagtccgg
     7741 ttccgggaag accacgctac tgaaatcaat ttccgcccgc ctgacaccac agcaagggga
     7801 aattcgctac gagaaccgtt cgctgtacgc gatgagcgag gccgaccgcc gtcgcctgct
     7861 gcgcaccgaa tggggtgtgg tgcatcagca tccgcttgac ggcctgcgcc gccaggtgtc
     7921 ggcaggcggc aatatcggcg aacggttgat ggcaaccgga gcgcgtcatt acggcgatat
     7981 tcgcgccacc gcgcagaaat ggctggaaga ggtggagatt cccgccaacc ggatcgacga
     8041 cctgccgacc accttttccg gcggcatgca gcaacgtttg cagattgccc gcaacctggt
     8101 gacgcatccg aagctggtgt ttatggatga acccactggc gggctggatg tgtcggtaca
     8161 ggcgcgcctg ctggacctgc tccgcggtct ggtgatggag cttaacctgg cggtggtgat
     8221 tgtcacccac gatttaggcg tcgcccgtct gctggcggac cgtttgctgg taatgaagca
     8281 agggaaagtg gtggagagtg ggttaaccga ccgggtgctc gacgacccgc accatccgta
     8341 tacccagttg ctggtgtcat cggttttgca gaattgagct ggtgccggat gcggcgtaaa
     8401 cgccttatcc ggcctacaaa tgcgctcccc gtaggtcgga taagacgcat cagcgtcgca
     8461 tccgacaccc gaaccacgag gcgaaaaatg attaacgtac aaaacgtcag taaaaccttc
     8521 atcctgcacc agcaaaacgg cgtgcgcctg cctgtattac aaggcgcctc actcaccgtc
     8581 aacgccggcg aatgcgtggt actccacggc cattccggca gtggaaaatc aactctgcta
     8641 cgctcgctgt atgccaacta tctacccgac gaagggcaaa tccagatcaa acacggtgat
     8701 gaatgggtcg acctggtcac cgcgccagcg cgcaaagtgg tggagatccg caaaaccact
     8761 gtcggctggg taagccagtt tctgcgcgtc atcccacgta tttcggcact ggaagtggtg
     8821 atgcagccgc tgctcgatac cggcgtaccg cgtgaagcct gcgccgctaa agccgcgcgt
     8881 ctcctcaccc gcctgaacgt gcctgaacgc ctgtggcacc ttgcgccatc gaccttctcc
     8941 ggtggcgaac agcagcgtgt caacatcgcc cgtggcttta tcgtcgacta ccccattctg
     9001 ctgctggatg aacccaccgc ctcgctggac gctaaaaaca gcgccgccgt agtggaactg
     9061 atccgcgaag ccaaagcccg cggcacggcc atcgtcggta tcttccatga cgaaaccgtg
     9121 cgcgagcaag tcgccgaccg cctgcaccca atgggagtct cttcatgatt atcaataacg
     9181 tcaaactggt gctggaaaac gaggtggtac acggttctct ggagatgcag gacggcgaaa
     9241 tccgcgcctt tgccgaaagc cagagtcacc tgtcagaagc aatggacggc gaaggcggct
     9301 ggctgctacc ggggctgatt gagctgcata ccgataatct ggataaattc ttcaccccgc
     9361 gcccgaaagt tgactggcct gcccactcgg cgatgagcag ccacgacgcg ctgatggtgg
     9421 cgagcggcat caccaccgtg ctggacgccg tggcgattgg tgatgtacgc gacggcggcg
     9481 atcggctgga gaatctggag aagatgatca acgccatcga agagacgcag aaacgcggcg
     9541 tcaaccgtgc cgagcaccgt ctgcacctac gctgcgaact gccgcatcac accacgctgc
     9601 cgctgtttga aaaactggta cagcgcgaac cggtaacgct ggtgtcgctg atggatcact
     9661 caccgggcca gcgccagttc gccaaccgcg aaaaatatcg tgaatattat cagggcaaat
     9721 actcgctcac cgatgcgcag atgcaacagt acgaagagga gcaactggcg ctcgccgcac
     9781 gctggtcaca gccgaatcgc gaatccatcg ccgcaatgtg ccgcgcgcga cacattgcgc
     9841 tcgccagcca cgatgacgcc acgcacgccc acgtagccca atcccatcag cttggcagcg
     9901 tgatcgccga atttcctacc acattcgaag cggcagaagc ctcgcgcaag cacggcatga
     9961 acgtactgat gggcgcgccg aatatcgtgc gcggcggttc gcactccggc aacgtggcgg
    10021 ccagtgaact ggcgcagctt ggcctgctgg atatcctctc ttccgactac taccccgcca
    10081 gcctgctgga tgcggcgttt cgcgtggccg atgaccagag caaccgcttt acgctgccgc
    10141 aggcggtgag gctggtgacc aaaaacccgg cccaggcact gaatctccag gatcgtgggg
    10201 tgattggcga gggcaaacgc gccgacctgg tgctggcgca tcgcaagggc aatcacattc
    10261 atattgacca cgtctggcgt cagggtaaaa gggtgttctg atgacgggaa aactgatttg
    10321 gttaatgggt ccgtccggct ccgggaaaga cagcctgctg gcggaactcc gccagcggga
    10381 acaaacccag ctactggtag cgcatcgcta catcacgcgt gatgccagcg ccggaagtga
    10441 aaatcatatc gccttaagcg agcgggagtt ttttacccgc gcggggcaaa atctgttggc
    10501 cttgagctgg catgctaatg gtttgtatta cggtgtcggc attgagatcg acctctggct
    10561 gcacgccgga ttcgacgtcg tggttaacgg ctcacgcgcc cacctgccgc aggcgcgggc
    10621 gcgttatcaa tcggcgctgc tgcccatttg cttacaggtt tcgccggaga tcctgcgcca
    10681 gcgcctggaa aaccgtggcc gtgaaaacgc cagtgaaatt aacgcccggc tggcgcgcgc
    10741 cgcccgctat actcctcagg attgccatac gctcaataat gacggcagcc tgcgccagtc
    10801 ggtcgacacg ctgctgacgc tgattcatca gaaggagaaa caccatgcct gcttgtgaac
    10861 ttcgcccggc aacgcagtac gacaccgacg cggtttacgc gctgatttgt gagctaaaac
    10921 aagcggagtt tgaccaccag gcgtttcgcg tgggctttaa cgccaacctg cgtgacccga
    10981 atatgcgcta ccatctggcg ctgttggatg gtgaggttgt cggcatgatc ggcctgcatt
    11041 tgcagtttca tctgcatcat gtcaactgga tcggcgaaat tcaggaactg gtggtgatgc
    11101 cgcaggcgcg cggtctgaac gtcggcagta aattactggc atgggcagaa gaagaagccc
    11161 gccaggccgg ggccgaaatg accgagcttt ccaccaacgt gaagcgccac gacgcacacc
    11221 gtttttacct gcgtgaaggc tacgagcaga gccacttccg cttcaccaag gcgctgtaat
    11281 atgagcctga ccctcacgct caccggcacc ggcggcgcac agggcgttcc ggcatggggc
    11341 tgcgagtgcg tagcctgcgc cagagcgcgg cgctcaccgc agtatcgccg ccagccgtgc
    11401 agcggcgtag tgaagtttaa cgaggcgatc accctgatcg acgccgagct gcacgatctc
    11461 gccgatcgct ggtcgcccgg atcgttccag cagtttttgc tgacgcacta ccatatggat
    11521 cacgtccagg ggctgtttcc gctgcgctgg ggcgtgggca atcagatccc ggtctacggc
    11581 ccgccggatg aacagggctg cgacgatctg tttaaacatc cgggcctgct tgatttcagc
    11641 cacacggtgg agccgtttgt ggtgtttgat ttgcaggggt tgcaggtcac gccgctgccg
    11701 cttaaccatt cgaaactcac tttcggctat ttactggaaa cggcgcacag ccgggtggcg
    11761 tggctatctg acaccgctgg cctgccggaa aaaacgctga aatttttacg caataatcag
    11821 ccgcaggtaa tggtgattga ttgcagccac ccgccacgcg aggatgcacc gcgtaatcac
    11881 tgtgatttaa ataccgtgct tgcgctgaat caggttatcc gctcgccacg ggtgattctg
    11941 acccatatca gccaccagtt tgatgcgtgg ctgatggaaa acgcactgcc gtcggggttt
    12001 gaggcgggat ttgatgggat ggagattgag gtggagtgag ggaaaatacc cgcgccggcc
    12061 cctacctcaa cgcccatact catcttccat tctccgctct tcatcttcca gttgccgacg
    12121 ctcctgatca agctggcgct ggcgatcgtc cagctgcctg cggcgatctt caaactggcg
    12181 gcggcggtca tcatattgtc tgcgccgatc gtcgctcact tcacgctgcc agccgtcgtc
    12241 gcgcgaatct tcatagtctc gcccacggtc agggttataa gcgtcgttaa tcgcctgctg
    12301 aatattgcca atggtgtcgt cgataatatc ggcctgggcc ggaacggtgg acagcgtgag
    12361 cagggtgaat aaaagaaata gcggaaagcg tttcattagc caacctcaaa aagaaactct
    12421 atccacatta gtcattactc atccatgcaa gtagtggatg aatctcaatt cctttgctgc
    12481 tctattgccg taatcgcctc cacgcgttgt tgatgacgac cgccttcgta ctgtgcgccc
    12541 agccacgcat cgacaatcat ttttgccagt tcgaggccaa ccactcgtga accaaaagcc
    12601 agcacgttgg tgtcgttatg ctgccgcgaa agttgcgcgg aataaggttc gctacaaacg
    12661 accgcgcgaa ttccggcaaa cttgttcgcc gctatcgaaa taccgacgcc agtgccacaa
    12721 atcaaaatcc cgccatcagc ctctccgcca gcaacggcca gtgcgacttg gctggcgtaa
    12781 tgcggataat cagtacgctc tgacgaccag gttcctttat caatcacttc aacgtcacgc
    12841 tcaactaaat gtgccactat ttcatgtttt aaaatgaaac cgacatgatc acagccaaat
    12901 gcaatctttt tcattcatct tccccgcccg tgcaaaactt cacaatcaaa aaacatttgt
    12961 gatgtaaatg aattaaaaac acccaaaatc gatgaaatcc gccttcccga tctgttttac
    13021 agagttacat ctggcagcgc aatagcgtta aagacactca ctatccagca acatctatca
    13081 tctaataaac caggaaaaca aataacatca tgtttttaaa gtgattaaat gaaacgaaat
    13141 tttaagctac tctccattat tcacaacaaa acaaacttta taaattttat ttttttgtga
    13201 agttaccagc atcttttctg ttcttgctgt ggtgatatag tggcgtcttc aattcaagga
    13261 caagagaacg tgatgagcca gtcagagttt gattcagcgc ttccgaacgg tatcgggtta
    13321 gcgccttacc tgcgaatgaa gcaggaagga atgacagaaa acgaaagccg catcgtggag
    13381 tggttactca aacccggtaa cctgagttgt gcacccgcaa ttaaagatgt cgcagaagct
    13441 ctggcggtat ctgaagcgat gatcgttaag gtatcaaagc tgctggggtt tagcggcttt
    13501 cgtaacttac gcagtgcgct ggaagattat ttttctcagt cagaacaagt attgccttcc
    13561 gagttggctt ttgatgaagc gccgcaggat gtggtgaata aggtatttaa catcacttta
    13621 cgcaccatta tggaaggtca gtcgatcgtc aacgttgatg agatccaccg tgccgcccgc
    13681 tttttctatc aggccagaca gcgggatttg tacggtgccg gaggatcaaa tgctatctgt
    13741 gctgatgtac agcacaagtt tttgcgcatt ggcgtacgct gccaggccta tccggacgct
    13801 cacatcatga tgatgtctgc ttcgttgtta caggaaggag atgtggtgct ggtggtgacc
    13861 cattccgggc gaaccagtga tgtaaaagcg gctgtagaac tggcaaaaaa gaacggggca
    13921 aagattattt gtataacgca tagctatcat tcaccaatag cgaagctggc cgattatatt
    13981 atttgctcac cggccccgga aacaccgtta ttaggtcgta atgcctcggc aagaatatta
    14041 caactgactt tgctggacgc gttttttgtc tctgtcgccc agctcaacat tgaacaagct
    14101 aatattaata tgcaaaaaac cggcgcaatt gttgatttct tctcaccagg cgcgctgaaa
    14161 taagaaaaag gtaattccgc catcgcggag ttacctctcc ccttaccgtg agaacatgat
    14221 tatgaataaa tatctgaaat atttcagcgg cacactcgtg ggcttaatgt tgtcaaccag
    14281 cgcttttgct gccgccgaat atgcagtcgt attgaaaaca ctctccaatc cattttgggt
    14341 agatatgaaa aaaggcattg aagatgaagc aaaaacgctg ggcgtcagcg ttgatatttt
    14401 tgcctctcct tcagaaggcg attttcaatc tcaattgcag ttatttgaag atctcagtaa
    14461 taaaaattac aaaggtatcg ccttcgcacc attatcctca gtgaacctgg tcatgcctgt
    14521 cgcccgcgca tggaaaaaag gcatttatct ggtcaatctc gatgaaaaaa tcgacatgga
    14581 taatctgaaa aaagcaggcg gtaatgtgga aggttttgtc accaccgata atgttgccgt
    14641 cggggcgaaa ggcgcgtcat tcattattga caagctgggt gccgaagggg gtgaagtcgc
    14701 aatcattgag ggtaaagccg gtaacgcctc cggtgaagcg cgtcgtaatg gtgccaccga
    14761 agccttcaaa aaagcaagcc agatcaagct tgtcgccagc cagcctgccg actgggaccg
    14821 tattaaagca ctggatgtcg ccactaacgt gttgcaacgt aatccgaata ttaaagcgat
    14881 ctattgcgcg aatgacacga tggcaatggg tgttgctcag gcagtcgcaa acgctggaaa
    14941 aacgggcaaa gtgttggtcg tcggtacaga tggcattccg gaagcccgca aaatggtgga
    15001 agccggacaa atgaccgcga cggttgccca gaacccggcg gatatcggtg caacgggtct
    15061 gaagctgatg gttgacgctg agaaatccgg caaggttatc ccgctggata aagcaccgga
    15121 atttaaactg gtcgattcaa tcctggtcac tcaataaaac tgaatgatgc cggatgatga
    15181 tgctaatgaa gtgtcttatc cggccaacaa aataacctga acgtagacct gataaacgta
    15241 gccatcaggc aataatacga tatttcgtgc agagaggtta tttatggcca cgccatatat
    15301 atcgatggcg gggatcggca agtcctttgg tccggttcac gcattaaagt cggttaattt
    15361 aacggtttat cctggtgaaa tacatgcatt actaggagaa aatggtgcgg gtaaatctac
    15421 gctaatgaaa gtgttatccg ggatacatga gccgaccaaa ggcaccatta ccattaataa
    15481 cattaactat aacaaactgg atcataaatt agcggcacaa ctcggtatcg ggattattta
    15541 tcaggaactc agcgttattg atgaattaac cgtactggaa aatttatata ttggtcgtca
    15601 tctgacgaaa aaaatctgcg gtgtcaatat tatcgactgg cgagaaatgc gtgtccgcgc
    15661 cgcaatgatg ttattacgcg tgggcttgaa agttgattta gatgagaaag tggcgaattt
    15721 atctatcagc cacaagcaga tgctggaaat tgccaaaacg ctgatgctcg acgccaaagt
    15781 catcatcatg gatgaaccca cctcctcact caccaataaa gaggtggact atctgtttct
    15841 gatcatgaat cagttgcgta aggagggtac ggccatcgtc tatatctcgc ataaattggc
    15901 ggaaattcgc cgtatttgcg accgctatac ggtgatgaaa gacggcagca gcgtttgcag
    15961 cggcatggtg agcgatgtgt caaatgacga tatcgtccgt ctgatggtag gccgcgaatt
    16021 gcaaaaccgt tttaacgcga tgaaggagaa tgtcagcaac cttgcgcacg atacggtttt
    16081 tgaggtgcgc aacgtcacca gccgtgacag aaaaaaggtc cgggatatct catttagcgt
    16141 ctgccgggga gaaatattag gctttgccgg actggtcggt tccggacgta ctgaactgat
    16201 gaactgcctg tttggcgttg ataaacgcgc tggcggagaa atccgtctta atggcaaaga
    16261 tatctctcca cgttctcccc tggatgccgt gaaaaaaggg atggcttaca tcactgaaag
    16321 ccgccgggat aacggctttt tccccaactt ttctatcgct cagaacatag ctatcagccg
    16381 cagtctgaaa gacggcggct ataaaggcgc gatggggttg tttcatgaag ttgacgagca
    16441 acgtaccgct gaaaatcaac gcgaactgct ggcgctgaaa tgtcattcgg taaaccagaa
    16501 catcactgaa ctctccggcg gtaaccagca gaaagtcctg atctccaaat ggctgtgctg
    16561 tcgcccggaa gtgatcattt tcgatgaacc tacccgtggc atcgacgttg gcgcgaaagc
    16621 cgaaatttac aaagtgatgc gccaactggc ggacgacgga aaagtcatcc tgatggtgtc
    16681 atctgaacta cctgaaatta tcaccgtctg cgaccgcatc gccgtgttct gcgaaggacg
    16741 actgacgcaa atcctgacga atcgcgatga catgagcgaa gaggagatta tggcatgggc
    16801 tttaccacaa gagtaaaaag cgaagcgagc gagaagaaac cgttcaactt tgcgctgttc
    16861 tgggataaat acggcacctt ttttatcctg gcgatcatcg tcgccatttt tggttcgctg
    16921 tcgtcagaat attttctgac caccaataat attacccaga tttttgtgca aagctccgtg
    16981 acggtactga tcggcatggg ggaattcttc gctatcctgg tcgccggtat cgacctttcg
    17041 gttggcgcga ttctggcgct ttccggtatg gtgaccgcta aactgatgtt ggcaggtatt
    17101 gatccgtttc tcgcggcgct aattggcggt gtactggttg gcggcgcact gggggcgatc
    17161 aacggctgcc tggtcaactg gacgggacta cacccgttca ttatcaccct tggcaccaac
    17221 gctattttcc gtgggatcac gctggtgatc tccgatgcca actcggtata cggcttctca
    17281 tttgacttcg tgaacttctt tgccgccagc gtaattggga tacctgtccc cgttatcttc
    17341 tcgctaattg tcgcgctcat cctttggttt ctgacaacgc gtatgcggct cgggcgcaac
    17401 atctacgcac tgggcggcaa caaaaactcg gcgttctatt ccgggattga cgtgaaattc
    17461 cacatcctgg tggtgtttat catctccggt gtttgtgcag gtctggcagg cgtcgtctca
    17521 actgcacgac tcggtgccgc agaaccgctt gccggtatgg gttttgaaac ctatgccatt
    17581 gccagcgcca tcattggcgg caccagtttc ttcggcggca aggggcgcat tttctctgtg
    17641 gtgattggcg ggttgatcat cggcaccatc aacaacggtc tgaatatttt gcaggtacaa
    17701 acctattacc aactggtggt gatgggcgga ttaattatcg cggctgtcgc ccttgaccgt
    17761 cttatcagta agtaaggaat tgaacatgaa aatctccccc tcgttaatgt gtatggatct
    17821 gctgaaattt aaagaacaga tcgaatttat cgacagccat gccgattact ttcacatcga
    17881 tatcatggac ggccactttg tgccgaatct gacgctctca ccgttcttcg taagccaggt
    17941 aaaaaaactg gcgagtaaac cgctcgactg ccatctgatg gtaacgcgtc cgcaggatta
    18001 cattgctcaa ctggcacgtg cgggagctga tttcatcact ttgcatccgg aaactatcaa
    18061 cggtcaggca ttccgcctga ttgatgaaat ccgccgtcat ggcatgaaag tggggctgat
    18121 tcttaatccg gaaacgccgg ttgaggccat gaaatactat atccataagg ctgataaaat
    18181 tacggtcatg actgtcgatc ccggttttgc cggacaaccc ttcattcctg aaatgctgga
    18241 taaagttgcc gaactgaagg catggcgtga acgagaaggt ctggagtacg aaattgaggt
    18301 ggacggttcc tgcaaccagg caacttacga aaaactaatg gcggcagggg cggatgtctt
    18361 tatcgtcggc acttccggcc tgtttaatca tgcggaaaat atcgacgaag catggagaat
    18421 tatgaccgcg cagattctgg ctgcaaaaag cgaggtacag cctcatgcaa aaacagcata
    18481 acgtcgtagc gggcgtggat atgggggcaa cgcatatccg cttttgtctg cggacagcag
    18541 aaggtgaaac gctacactgc gaaaaaaagc agaccgcaga agttattgct cccgacctgg
    18601 tgtcgggtat cggcgaaatg attgacgagc aactcaggcg ctttaacgct cgctgtcatg
    18661 gtctggtgat gggatttccg gcgctggtca gtaaagataa acgcaccatt atttctacgc
    18721 ctaacctgcc gttaacagcg gcagatttat atgatctcgc cgataagctc gaaaatacgc
    18781 tgaattgtcc ggttgagttt tcccgcgacg ttaacctgca actctcctgg gacgtagtag
    18841 aaaatcgcct tacgcaacaa ctggttctgg cagcctatct cggtacgggg atggggttcg
    18901 cagtgtggat gaacggtgcg ccgtggacgg gtgcacacgg tgtggcaggc gaactgggcc
    18961 atatccccct aggagatatg acccaacact gcgcgtgtgg taatcctggg tgcctggaaa
    19021 ccaattgctc tggaatggcg ctaagacgct ggtacgaaca acagccccga aattacccat
    19081 tgagcgatct tttcgtccat gcggaaaacg cccctttcgt ccagagtctg cttgaaaacg
    19141 cggcacgagc cattgccacc agcattaatc tgttcgatcc cgatggggtg attctgggtg
    19201 gtggcgtgat ggatatgccc gccttcccac gcgagactct cattgccatg acccaaaagt
    19261 acctgcgccg tccactgccg tatcaggtcg tgcgctttat tgccgcctca tcttctgact
    19321 ttaatggcgc tcagggtgca gcaatattgg cgcatcaacg ttttttgcca cagtcctgtg
    19381 ctaaagtccc atgagtaaag cgttggcaca gtagcgcaat gtcccgctgt gccaacgaca
    19441 ggtacaatat atataataat tacataaaac agccatatta ttcccagcaa tgatagttct
    19501 actgtaattt tccctcttca gcacaaatgc ctaataaaat ggctcgttcc gctcacactt
    19561 ccacttatta aagtcgcaaa ttcccaatta tgataacgcc agctttaaat gacgtatttt
    19621 gcatggatta catatttacg acataaatga taaatggaga tttatatgaa taactctcgg
    19681 ttattccgtt tgagcaggat tgttattgcg ttcactgccg ccagcggcat gatgataaat
    19741 accgcttacg caacagatga ggcgaaagcc gccactcaat atacccaaca ggttaatcag
    19801 aattacgcca aatcattacc gtttggcgat cgccaggatt ttgacgatgc tcagcgtgga
    19861 tttatcgctc cgctgctgga tgaaggtatt ctgcgtgatg cgaacggtaa aatttactac
    19921 cgcgcgaatg attacaaatt tgatattaat gccgctgcgc cggaaaccgt aaaccccagc
    19981 ctgtggcgtc agtcgcagat caacggcatt tctggcctgt tcaaagtcac cgataaaatg
    20041 tatcaggtgc gcggccagga tatctctaac attacgttcg ttgagggcga gaaaggcatt
    20101 attgttattg acccactggt gacgccacct gccgcaaaag ccgcacttga cctttacttc
    20161 cagcatcgtc cgcaaaaacc gattgttgcc gttatctaca ctcacagcca cgccgaccac
    20221 tacggcggcg tgaaaggtat tatctctgaa gccgatgtta aatccggcaa agttcaggtg
    20281 attgctccag ctggctttat ggacgaagcc atcagcgaaa acgtgctggc gggtaacatc
    20341 atgagccgcc gtgcgctcta ctcttacggt ctgttactgc cgcacaacgc gcaaggcaat
    20401 gtgggtaatg gccttggcgt gacgctggca acgggcgacc cgagcattat tgcaccgact
    20461 aaaactatcg tcagaactgg cgagaagatg attatcgacg gcctggagtt tgacttcctg
    20521 atgaccccag gtagcgaagc gccagcggaa atgcacttct atattccggc cctgaaagcc
    20581 ctgtgtaccg ccgagaatgc cacgcatacc ctgcacaact tctacaccct gcgcggcgcg
    20641 aaaacccgcg acaccagcaa gtggaccgag tatctgaacg aaacattgga tatgtggggt
    20701 aacgacgcgg aagtactgtt tatgccgcac acctggccgg tctggggcaa taagcatatc
    20761 aatgattata ttggtaaata tcgcgatact atcaagtaca ttcacgacca gaccctgcac
    20821 ctggcgaacc agggctacac catgaatgaa atcggcgaca tgattaagct gccgcctgcg
    20881 cttgccaata actgggccag ccgcggctat tacggttctg tcagccacaa cgcccgcgcg
    20941 gtatataact tctatcttgg ctattacgac ggtaacccgg ctaacctgca tccgtatggt
    21001 caggtggaga tgggtaaacg ttacgtgcag gcgctgggcg gttctgcccg cgtcatcaac
    21061 ctggcgcaag aagcgaacaa gcaaggtgat tatcgctggt cggcagaact gctgaaacag
    21121 gtgattgccg ccaacccggg tgaccaggtc gcgaagaatc tgcaagcgaa taactttgaa
    21181 cagctgggct atcaggccga gtccgccaca tggcgcggtt tctacctgac cggcgcgaaa
    21241 gagctgcgcg aaggggtgca taagttcagc cacggcacca ccggttcccc ggacaccatt
    21301 cgtgggatgt cggtcgaaat gctgttcgac tttatggccg ttcgcctcga tagcacgaaa
    21361 gccgcaggta aaaatatcag cctgaacttc aatatgagca acggcgataa cctcaacctg
    21421 atgctgaacg atagcgtgct taactaccgt aaaacgctgc aatcgcaagc cgacgcctct
    21481 ttctacatta gccgcgaaga tctgcacgcc gtgctgaccg gacaggctaa aatggccgat
    21541 ctggtgaaag cgaagaaagc caaaattatt ggcaatggcg cgaaactgga agaaattatc
    21601 gcctgtctgg ataatttcga tttgtgggtg aatatcgtaa ccccaaatta atgtcactcg
    21661 cttttggggg cgctgaagcc cccaaaaaca tttgctttaa aaatatatac agccaattaa
    21721 taaataaaat atataagtta tgaatgcggt ggctgaataa agctattttt attcataggg
    21781 aaaatagcca tactgacgcc ttcaatggat attgatattc actacagata ttgttcgcag
    21841 cagacaggat gtatttaata tgccaacagt tctctctcgc atggcgatgc aactcaaaaa
    21901 aacagcctgg ataattcccg tcttcatggt ttcgggatgc tcattatctc cggcaatccc
    21961 ggtgatcggc gcttattatc ccggctggtt tttctgcgct attgccagcc ttattttgac
    22021 gctcatcacg aggcgaatta ttcagcggac aaatatcaat ctggcatttg tcggaattat
    22081 ttataccgcc ctttttgctc tctacgccat gctgttctgg ctggcatttt tctaatctat
    22141 ttgagacgcg ctcatggaaa gtacgccgaa aaaagctcct cgcagtaaat tccctgctct
    22201 gttagtggtt gcgttggcgc tggttgccct tgttttcgtt atctggcgcg tagacagtgc
    22261 gccatcaact aatgacgctt acgcgtcagc agataccatt gatgtggtgc cggaagtcag
    22321 cggtcgcatt gtagaactgg cggtcaccga caaccaggcg gtgaaacagg gcgatttgct
    22381 gttccgcatc gacccgcgcc cgtacgaagc caatctggcg aaagccgaag cctccctcgc
    22441 ggcgctggat aagcaaatta tgctcaccca gcgtagcgtt gacgcacaac agtttggtgc
    22501 cgactcggtt aatgccacgg tagaaaaagc ccgtgccgcc gcgaaacaag ccagcgatac
    22561 gttacgccgt accgaaccgc tactgcgaga aggttttgtt tctgcggaag aggttgaccg
    22621 tgcaagaacc gcacagcgtg cggcagaagc ggaccttaat gccgtattgt tgcaggcgca
    22681 gtcagccgcc agcgccgtca gcggcgtgga tgcgctggtg gcgcagcgtg cggcagtgga
    22741 agcggatatt gccctgacca aactgcatct ggaaatggcg accgttcgcg cgccgtttga
    22801 tggccgggtc atttccctca aaacctccgt cgggcagttt gcatctgcga tgcgccctat
    22861 tttcactcta atcgacactc gtcactggta cgtgattgcc aacttccgcg aaactgatct
    22921 gaaaaatatt cgctcgggta cactcgcaac cattcgcctg atgagcgaca gcggcaaaac
    22981 cttcgagggt aaagtggatt cgattggcta cggcgtgcta ccggatgacg gcggcctggt
    23041 gctgggcggc ctgccgaaag tgtctcgttc tattaactgg gtccgcgttg cccagcgttt
    23101 tccggtcaaa atcatggttg ataaacctga cccggaaatg ttccgcatcg gcgcttcggc
    23161 agtcgctaat cttgagccgc aataatgagc gcgctcaact ccctgccatt accggtggtc
    23221 aggctgctga cgttctttca tgaagagtta agcgagcggc gaccagggcg cgtgccacag
    23281 acgatgcaac tctgggtagg ttgcctgctg gtgattctga tctcgatgac ttttgagatc
    23341 ccttttgtgg cgttatcact ggcagtgctg ttttacggta ttcagtcgaa cgcgttttac
    23401 accaaatttg tggcgatctt gtttgtggtc gccacggtgc tggagatcgc cagcctgttt
    23461 ttgatctaca aatggtcata tggcgaaccg ttgatccgat tgatcatcgc cgggccgctc
    23521 ctgatgggct gcatgttttt gatgcgcacc catcggctgg gactggtctt tttcgccgtc
    23581 gccattgtcg ctatttacgg gcaaaccttc cccgccatgc tcgactatcc ggaagtggtc
    23641 gtgcgcttaa cgctgtggtg tatcgttgtt ggcctctatc caaccttgct gatgacgtta
    23701 atcggcgtgc tgtggtttcc cagtcgtgcc attacgcaga tgcatcaggc gcttaatgat
    23761 cggcttgatg atgccattag ccacctgacg gacagcctcg caccgctacc cgaaacgcgg
    23821 attgaaagag aggcgctggc gctgcaaaaa ctcaatgtct tttgcctcgc ggacgatgcc
    23881 aactggcgaa tccagagcgc atggtggcaa agctgcgtgg caacggtaac ctacatttac
    23941 tcgacgctaa atcgctacga tcccacctct tttgctgatt ctcaggcaat tattgaattc
    24001 cgacaaaaat tagcttcaga aatcaacaat ctgcagcatt ccattaccga aggtcagtgc
    24061 tggcaaagcg actggcggat cactgaaagt gaagcgatgg cggcacggga atgtaacctg
    24121 gagaatatct gccagacgct gttacagctg ggtcagatgg acccgaatac gccgccaacg
    24181 cccgcagcca aaccgccatc aatggtcgcc gatgctttta ccaatccaga ctatatgcgc
    24241 tacgcggtaa aaacgctgct cgcctgtttg atctgttaca ccttctacag cggcgtggac
    24301 tgggaaggca ttcacacctg tatgctgacc tgcgtgatcg tcgctaatcc aaatgtcggt
    24361 tcgtcgtacc agaagatggt gctgcgtttt ggtggggcct tttgcggcgc gattctggcg
    24421 ctgttattca cgctactggt catgccctgg ctggacaata ttgtcgaatt gctgtttgtg
    24481 ctggcaccga ttttcctgtt gggcgcatgg attgccacca gctctgaacg ctcttcttat
    24541 atcggcacac agatggtggt caccttcgcg ctcgccacgc tcgaaaacgt ttttggcccg
    24601 gtgtacaacc cggtggaaat tcgcgatcgc gctctgggta tcatcattgg taccgtggtg
    24661 tcggcggtga tttacacctt tgtctggcct gaaagcgaag cgcgcacgct gccgcaaaaa
    24721 ctggctggcg cgctgggtat gttgagtaaa gtaatgcgga tcccacgcca gcaggaagtc
    24781 acggctctgc gcacttatct gcaaattcgc ataggtctgc atgcggcgtt taatgcctgt
    24841 gaagagatgt gccaacgcgt ggtgctggag cgtcaactgg acagcgaaga acgcgcatta
    24901 ctgattgaac gttcgcaaac ggttattcgt cagggccgcg atattcttca cgcctgggat
    24961 gccacctgga actcggcgca ggcgctggat aacgcactac agccggacag agcaggtcag
    25021 tttgccgatg ccctggagaa atacgccgcc ggtgtggcta ccgcactcag tcattctcct
    25081 caaataacgc ttgaagaaac acccgcctcg caggccatcc tgccgacctt attaaaacag
    25141 gagcaacacg tctgccagct tttcgcccgc ttgccagact ggacagcccc ggcattaacg
    25201 cccgccacgg aacaggcaca aggagccacg caatgatcaa tcgtcaactt tcacgcctgc
    25261 tgttgtgcag cattctcggc agcacgacgc tgatttccgg ctgtgccttg gtacgtaagg
    25321 attctgcgcc tcatcaacag ctcaaaccgg aacaaatcaa actggccgat gatattcatc
    25381 ttgccagctc cggctggccg caggcgcagt ggtggaaaca atttaacgac ccgcagctgg
    25441 atgcgctgat ccaacggacg ctaagtggtt cacaccccct cgccgaagcg aaactgcggg
    25501 aagaaaaagc gcagtcccag gccgatttgt tagatgccgg ttcacaatta caggtcgcag
    25561 cgttagggat gctcaaccgc caacgcgtct cggcgaacgg ctttttaagc ccttatgcga
    25621 tggatgcgcc agcactgggt atggacgggc cgtactatac ggaagccaca gtcggtttgt
    25681 ttgccggact ggatctcgat ttgtggggtg tgcatcgctc agcggttgct gccgccattg
    25741 gcgcgcataa tgccgcgctg gcagaaaccg cagcagtaga gctatcgctg accacgggcg
    25801 tagcgcagct ttattacagt atgcaggcca gctatcagat gctcgatctg ttagaacaaa
    25861 ctcgcgatgt gattgattac gcggtgaaag cgcaccagag taaagtggcg cacggtctgg
    25921 aagcgcaagt gcctttccac ggcgcgcggg cacagattct ggcggtcgat aaacaaattg
    25981 ctgccgtcaa agggcaaatc actgaaacgc gagaatctct gcgtgcattg attggcgcgg
    26041 gagccagcga tatgccggag atcaaaccgg tggcattacc acgagtacag accggcattc
    26101 cggcaacgct ctcttatgag ttgctcgcca gacgcccgga tctgcaagcc atgcgctggt
    26161 atgttcaggc gtcattagat caggtggatt ccgcgcgggc gctgttctac ccgagctttg
    26221 atatcaaagc gtttttcggt ctggactcca tccacctgga taccttattc aaaaaaacca
    26281 gtcgccagtt caacttcatc cctggtctga aattgccgct gtttgacggt ggacggttga
    26341 atgccaatct cgaaggcact cgcgccgcca gcaacatgat gattgaacgt tacaaccagt
    26401 cagtactgaa cgcggtgcgt gacgttgccg tcaacggcac acgtctgcaa acgctcaacg
    26461 acgagcgaga gatgcaggct gaacgcgtag aagcaacgcg cttcacccag cgcgcagccg
    26521 aggccgccta tcagcgcggc ttaaccagcc gcttacaggc caccgaagcc cgattgccag
    26581 tgcttgccga agagatgtca ttactgatgc tggacagccg ccgggtgatc caaagcattc
    26641 agttgatgaa atcgctgggc ggcgggtatc aggcggctcc cgtcgtcgag aaaaaataaa
    26701 atgtctaccg cgtgatggct gtcacgcggt atttcgtttc gtcacgtcaa aactgacgac
    26761 agcctgtttt tcgtcagagt tttgaataaa tagtgcccgt aatatcaggg aatgacccca
    26821 aataaaatgt ggcataaaag atgcatgctg tagtcgagag cgcgtatgcg tgatttgatt
    26881 aactggagcg agaccgatga aaaaagtcgt cacggtttgc ccctattgcg catcaggttg
    26941 caaaatcaac ctggtcgtcg ataacggcaa aatcgtccgg gcggaggcag cgcaggggaa
    27001 aaccaaccag ggtaccctgt gtctgaaggg ttattatggc tgggacttca ttaacgatac
    27061 ccagatcctg accccgcgcc tgaaaacccc catgatccgt cgccagcgtg gcggcaaact
    27121 cgaacctgtt tcctgggatg aggcactgaa ttacgttgcc gagcgcctga gcgccatcaa
    27181 agagaagtac ggtccggatg ccatccagac gaccggctcc tcgcgcggta cgggtaacga
    27241 aaccaactat gtaatgcaaa aatttgcgcg cgccgttatt ggtaccaata acgttgactg
    27301 ctgcgctcgt gtctgacacg gcccatcggt tgcaggtctg caccaatcgg tcggtaatgg
    27361 cgcaatgagc aatgctatta acgaaattga taataccgat ttagtgttcg ttttcgggta
    27421 taacccggcg gattcccacc caatcgtggc gaatcacgta attaacgcta aacgtaacgg
    27481 ggcgaaaatt atcgtctgcg atccgcgtaa aattgaaacc gcgcgcattg ctgacatgca
    27541 cattgcattg aaaaacggct cgaacatcgc gctgttgaat gcgatgggcc atgtcattat
    27601 tgaagaaaat ctgtacgaca aagcgttcgt cgcttcccgt acagaaggct ttgaagagta
    27661 tcgtaaaatc gttgaaggct acacgccgga gtcggttgaa gatatcaccg gcgtcagcgc
    27721 cagtgagatt cgtcaggcgg cgcggatgta tgcccaggcg gaaagcgccg ccatcctgtg
    27781 gggcatgggg gtaacccagt tctatcaggg cgtggaaacc gtgcgttctc tgaccagcct
    27841 cgcgatgctg accggtaacc tcggtaagcc acatgcgggt gtaaacccgg ttcgtggtca
    27901 gaacaacgtg cagggtgcct gcgatatggg cgcgctgccg gatacgtatc cgggctatca
    27961 gtacgtgaaa gatccggcta accgcgagaa atttgccaaa gcctggggcg tagaaagcct
    28021 gcctgctcat accggttatc gtatcagcga gctgccgcac cgcgtggcgc atggtgaagt
    28081 gagagcggcg tacattatgg gcgaagatcc gctgcaaact gacgcggaac tgtcggcagt
    28141 acgtaaagcc tttgaagatc tggaactggt tatcgttcag gacatcttta tgaccaaaac
    28201 cgcgtcggcg gcggatgtca ttttaccgtc aacgtcgtgg ggcgagcatg aaggcgtgtt
    28261 tactgcggct gaccgtggct tccagcgttt cttcaaggcg gttgagccga agtgggatct
    28321 gaaaacggac tggcaaatca tcagtgaaat tgccactcgt atgggttatc cgatgcacta
    28381 caacaacacc caggagatct gggatgagtt gcgccatctg tgcccggatt tctacggtgc
    28441 gacttacgag aaaatgggcg aactgggctt cattcagtgg ccttgccgcg atacttcaga
    28501 tgccgatcag gggacttctt atctgtttaa agagaagttt gataccccga acggtctggc
    28561 gcagttcttc acctgcgact gggtagcgcc aatcgacaaa ctcaccgacg aatacccgat
    28621 ggtactgtca acggtgcgtg aagttggtca ctactcttgc cgttcgatga ccggtaactg
    28681 tgcggcactg gcggcgctgg ctgatgaacc tggctacgca caaatcaata ccgaagacgc
    28741 caaacgtctg ggtattgaag atgaggcatt ggtttgggtg cactcgcgta aaggcaaaat
    28801 tatcacccgt gcgcaggtca gcgatcgtcc gaacaaaggg gcgatttaca tgacctacca
    28861 gtggtggatt ggtgcctgta acgagctggt gaccgaaaac ttaagcccga ttacgaaaac
    28921 gccggaatac aaatactgcg ccgtgcgcgt cgagccgatc gccgatcagc gcgccgccga
    28981 gcagtacgtg attgacgagt acaacaagtt gaaaactcgc ctgcgcgaag cggcactggc
    29041 gtaataccgc ttaaactcac gttaaaagcg gcgatattac ccaacgcctt tgtatcaatc
    29101 cggaaacgac tctctgtttc cggatttttt tatacatacc tgcgcagtca aaccagccca
    29161 tcctggttat tagcgacgtc ttctcggttg ttcttctatt ccagtcacga ttgcaaatga
    29221 gaattatctt tactcaattc tcatcgcggc ctaacgtaat aatactttac atttgataaa
    29281 caaaatttcg gtaaggtgag aaatcatcga ccgtttaact cgttctatta acgggatatt
    29341 ttggcgcgat taatgcagcg aacccccgtc tgatagcata ttaataacct caaagtaatt
    29401 tcagggattg ggaagcatat gattaaaaaa ctattacctt tactggtttt aagcacaatt
    29461 tccgccacaa ccgttgccgc tacaccgccg aatacgttgg ttgtagccca gggactggat
    29521 gatattgtga gtctggaccc ggcagaagct aatgaactct caagtattca gactgtacca
    29581 agtctgtatc aacgcgtagt acaaccggac cgtaatgatc cgacgaaaat cactccgatt
    29641 ctggcggaaa gttggcaggc tgaccccgca gccaaaacgc taaccataaa actgaaaagc
    29701 gatgcgaaat ttgcctctgg taatccggta cgcccggaag atgtgattta ctcttatgtc
    29761 cgggctgtca cactgaataa atccggtgcg tttattctca atgtgctggg atggcagccg
    29821 gaaaatatcg ccagccagtt gaagaaaatc gatgaccata ccgtccaggt gcaatggagt
    29881 gccgacgtca gcccggcgct ggcactgaat attctctcca caccaatcgc ctcaattgtt
    29941 gacgagaagc tggttgcgcc aaacgccaaa aataacgact ttggtaatga atggctgaag
    30001 atgcattccg ctggcagcgg cgcgtttaaa atgcgtactt accaaccgca tcaggccatc
    30061 gtgatggaag ccaacaccag ctcaccaacc ggcgccccga aactgaaaag cgtcatcatc
    30121 aaaaatgttc ccgatccggc aacgcgtcgc ctgctgatcc aacaaggtga tgctgatatg
    30181 gctcgtgatc tgggagccga tcaaatcgat gcattacagg gtaaacctgg cgtaaaagtg
    30241 atgagcattg cctccgccga acagaactac ctggccttca ataccggtaa caaggataac
    30301 ccgctgatga gtaatcctgc gttctgggag gccgctcgct ggttggtcga ttacgacggc
    30361 atcaccaaaa atctcttaaa aggacaatat tttacccatc aaagtttcct gcccgtgggc
    30421 tttcctggag cactggaaga aacaccgttt acctttaatc cagccaaagc aaaagagatc
    30481 ctcgctaaag ccggaatcaa agacccgcac ttcacgcttg atgtcgaaaa taaaccgcca
    30541 tttattacca tcgcgcagtc aattcaggcc agctttgctc agggcggtgt aaaagttgat
    30601 ttgctgccag ccgcagggag tcaggtctac tctcgcgttc gcgcccacca gcatcagggg
    30661 gccattcgta tgtggttgcc tgattatttt gacgcacatt ccaacgccag ctctttcgcc
    30721 tataacgatg gcaaatccag caccgttgcc gggttaaacg gctggaaaat cccagaactg
    30781 aacaaagaaa cgctggcggc catcgctgaa cctgatagcg ctaaacgtct tgatttgtat
    30841 aagaaaatgc aacaagaact acaacgtagt tccccgtatg tctttattga tcaaggcaaa
    30901 acgcaaattg tcatgcgtga taatgtgcag gggtatcaac aagggttgaa tgccgatatg
    30961 gtctggtttg atcaggtaac caaataaccc tgtatgcaac tcgcgtccag ccatgttacc
    31021 gggcgcggta ccagggctgg cctgtattgc aggccagccg ctgatttatc actgtatgga
    31081 aagatcatgt ctgtcgtttt ttccactcgc gcgagatggc gaggcaagcg ccctctgctc
    31141 gcgctgttgc aagggctgtt taccgttgct ctgacgctac tcggcctgct gctgattacc
    31201 tttgcgcttt cggctctctc accggtcgat cgcgtactac aaatcgttgg cgatcacgcc
    31261 agccagtcca cctatgatca ggtacgccac cagttaggat tggatcagcc gttagccgtc
    31321 cagttctggc attacctgat gaatctggca cacggcgatc tgggcatcgc cagctccacc
    31381 gtgcaaccag tactccagga cttgctatca gcattcccgg cgaccattga actggcaacg
    31441 ttggcgctta ttgtcggtgc tgttgtcggc gtcattgcgg gtgtactttg cgcccgttat
    31501 gtcggttctc cgctggattt caccgtgcga acattaactc tactgggaaa ttccgtccct
    31561 gttttttggc ttggtctgct gatgctggcg ctgttctacg ccagactgca atggagtgcc
    31621 gggccgggca ggctggatga tatttatcaa ttcaccatag aaccccgcag cggatttgcg
    31681 ctgattgata cgtggctttc tggcgatcgc gaggcattta ccaatgcaat caaccatttg
    31741 atacttccgg ttttattact ggcgtacttc tcgctcgcca gtattacgcg cctgactcgt
    31801 tccgcctgtc ttggcgaaat gaataaagaa tatattctgc tggcgcgcgc caaaggggcc
    31861 agcgagatga caattttgct acgtcatgtt ttacccaaca ttcgcggcac cttgctcacc
    31921 gttatcgccc tcgcctatac atcaatgctg gaaggcgcgg tactgacaga aaccgtcttt
    31981 tcatggccag gtattgggcg ctacctcact acagcgctct tcgcgggtga taccacagca
    32041 atcatgggag gaacgctgct gattggcgtc tgctttgtgt tgataaataa cttaaccgac
    32101 ctgctggtcc ggctgaccga tccgaggtta cgctgatgcc gatgtttctc tttttacgcc
    32161 gtttacgcca ttcacccgcc gcgttttgcg ggttgataat tatcctgtta ttgatgctaa
    32221 ttgcgctctt cgcgccgtgg ctggcacctc acgatcctaa ctggcaggat gcggcggcgc
    32281 ggttacaagc accgagtcgt cagcactggc tcggcactga cagttacggg cgagacctac
    32341 tttcacgttt aatctacggc agccgcccgg cactggggct ggtggcattg gtgacaatca
    32401 ttacactccc ggtcggtctg ctgattggca ttttagccgg gtactacggc ggctggctgg
    32461 aacgcgtgct gatgcgtttt actgacgtag taatgtcaat gccgcgttta attctggctt
    32521 ttgcttttgt tgccatgctg gggccggggc tggttaacgg cgcactggcg ctggcattaa
    32581 ccacctggcc tgcttatgcg cgtcaggcac ggagtgagat ccaacgtctg cgacacagtg
    32641 attatcttgc cgccgcagaa atgatgggca tccgcggcag gcgtctgctg gctggtcata
    32701 ttttaccatt gtgcctgcca tctgccattg tccgtctggc gctggattta gcgggtatca
    32761 ttctggcggc tgctggcctg ggttttcttg ggcttggtgc tcgcccgcca atggcggaat
    32821 ggggagccat gatcgccgac ggtatgcagg tcatttttga tcaatggtgg attgccgccg
    32881 ctcccggcgc aattatctta attgccagtc tggcctttaa tctgctgggt gatggcttgc
    32941 gcgatgttct ggagcctcaa catgactgac tcacgtctta gtatcaacaa tctctgcgtc
    33001 gattatccgg agtcccgggt cgtcaacaat gtcagtttta cattaggcaa tgaacgtctg
    33061 gcgctggtcg gtgagtcggg ttcaggtaag tccatgaccg cccgcgccct gatggggctg
    33121 gtgcgtaaac cgggtgtagt aaccgcggac acacttaaca ttctggggcg tgatgcactc
    33181 acccttaacg cgcgtggatg gcaacagttg cgcggtaacg atatcgcgat ggtattgcaa
    33241 gatccgcgtt acgcgcttaa tccagtaaag acagtaaaag cgcaactgga agaggcgctt
    33301 acgctccatc aacgtctgaa ccgccgcgag aaagaagaga aaataaacac cgcaattcag
    33361 gctgtcggcc tcaatacgcg agtactgcaa agctatcctc gtgaactttc tggcggcatg
    33421 gggcagcgag tgatgattgc aattgcgctc atcaacgatc cccgggtatt gattgccgat
    33481 gagccaacat cagcactgga tgtgcgcctg cgtaatcaga ttctggaact gctggttacc
    33541 cagtgtgaac aacgtcagat ggcaatgttg ctgatcagtc acgacctgcc attagttgcc
    33601 caattttgcc atcgggtgct ggtgatgtat cagggaaata aggtggatga aatgcacgct
    33661 gctgcgctac ctacagcaac gcatccttac acgcgcacac tgtggacttg ccgcccaaat
    33721 gcacaaactt acgggcagat gctgccgacg ctggacagga cggcaatgac gccggagaaa
    33781 taccatgacg attgttgaaa ttaatcactt acaggtgact tttgctgaga aaaccgcagt
    33841 ttctgccgcc agtttttcgg ttaatgccgg agagacattt agtttaattg gcgaatcagg
    33901 ctgcgaaaaa tccacgattt tgcgcgtact tgctggatta cagcgcgact ggcacggtca
    33961 ggtgtcactg tttgggcaca ccatcaaacc aggaatgcgt tttcagggcg atctccgccg
    34021 caatgtgcaa atggtgtttc aggatcccta tgcctcgttg catcccaatc atactctgtg
    34081 gcgaacgctg gctgaaccac tcaaaattcg tggcgaaaga gaaatagaaa aacgcgtcca
    34141 gactgcactg gaacaggttg gcttgccttt cgacgccgct cgtcgctacc cacaccagct
    34201 ttccggtggt caacggcaac gtgtggttat tgctcgcgcc ctgttgttac gaccacaact
    34261 tcttctgctg gatgaaccca cctcggcgtt ggatatgtcg gtacaagcgg aaatccttaa
    34321 tttgttgaat caacttaagc tggaacacca aatgacatat ctgctggtca gtcacgatgc
    34381 cgatgtcatc gcgcatatgt ctgatcgagc ggcattgatg tccgagggaa aaattcagcg
    34441 attttttgat cgcgacgcca tggaaaaggg ggaacatcgg atggattaaa aaacctgaca
    34501 ttgcattaat tcattctgct ttcaggtctt tttacagttg tttttttgtc cacccgaaca
    34561 ctttatactg tctctcccct accaaacttc attatcaaaa tgaaaaaaat tatcactttg
    34621 atgttatttt tgacattctt tgcccacgcg aacgactccg agcctggcag ccagtattta
    34681 aaggcagcag aggccagaga ccgacgcgca caatattttc ttgccgacag ctggtttagc
    34741 tccggcgatt tgagcaaagc cgaatattgg gcacagaaag ccgccgacag cggtgatgcc
    34801 gatgcctgcg cgctgttggc gcagatcaaa atcaccaatc cggtcagtct ggactaccca
    34861 caagcaaaag tgcttgccga gaaagcagcg caagctggca gtaaagaagg tgaagtaacg
    34921 ctggcgcata ttctggtaaa tacgcaagcg ggtaaaccgg attatccaaa ggcgatttcg
    34981 ctgttagaaa acgcctcgga agatctggag aacgactctg ccgtcgatgc ccaaatgctg
    35041 cttggtttga tttacgccaa cggcgtgggc attaaggccg acgatgacaa agcaacctgg
    35101 tatttcaaac gtagctccgc gatttcccga accggttatt ccgaatactg ggcgggaatg
    35161 atgttcttaa acggtgaaga gggttttatc gagaagaaca aacaaaaggc gctgcactgg
    35221 ttgaacctga gctgtatgga agggtttgat accgggtgtg aagagtttga aaaattaacc
    35281 aacggttaag gtaggcgcag caaacgtcgc atcaggcgtt gatgtcggat gcggcgtaaa
    35341 cgccttatcc gacctacaaa aaccacgcta catcaggcta ttactcagtc aggcatcaac
    35401 acattgccgg gtggatatcc ccggcaatct tcagttactg atccgccgtt ttatcaaatt
    35461 tgcccagcac ttcacgctca taagccagcg ctttcttacg gtcaaatttg tgttcccatt
    35521 tggcaatcac cagcaccgcc agcgcattac ccaccacgtt cagcgcagta cgcgccatgt
    35581 cgaggatacg gtcaacaccg gcaataaacg ccagaccttc cagcggaata cctacgctac
    35641 ccagcgttgc cagcagcacc acaaacgaca cgcccggcac gccagcaatc cctttcgagg
    35701 tcaccatcag cgtcagcacc agaatgattt cctgccagat ggacaggtca atgccataca
    35761 gctgagcgat gaaaatagcg gcaatacttt gatacagcgt cgaaccatca aggttaaaag
    35821 agtaaccggt cggcaccacg aaactggtga tcgacgccgg tgctccgtag gcttccatct
    35881 tctcaataat tcgcggcagc acgctttcag agctggcagt ggagtacgcc agaatcagct
    35941 catctttcag aatacgaatc aggatccaga cgcttaaccc gcacaggcgc gccacaattc
    36001 ccagcactac cagcgcgaag aacagaatgg cgaaatgcac cagcagcacc agtttcgcca
    36061 gtggccacag agacgagaaa ccaaagttag ccaccgtcac cgcaatcagc gcaaacacac
    36121 ccaccggcgc ataacgcatc accatgtgag tcactttaaa catggtttca gagatggagc
    36181 ggaaaacggt caccagcggt tcacgatgcg tcgcgggcag ggatgaaagc cccagaccaa
    36241 acagcaccga gaagaagatg atcggcagca tttcgccttt cgccatcgac gccacgatgt
    36301 tcgtcggcac cagcgacaaa atcgtgccca taatgccgtg ggaactgctt tgtaccgctt
    36361 ccgtggtgct ctgatatttc gagatatcga cggtcgccag ctgcgacata tccaccccgg
    36421 caccgggctg gaagacgttc gccagagtga tccccaaaat gatggcgacg gtggtgatca
    36481 cctcgaagta gataatggtt ttcgcaccaa tacgcccgag ctgtttggca tcaccaacac
    36541 ccgcgatacc caccaccagc gtggagatca caatcggcac aacaatcatt ttgatcagat
    36601 ggatgaagat atcacccgcc ggagagagca aattgacgac cagccagtcg cggctgtcgc
    36661 tatggtagtg caggtagctt cccaggagaa tgcccagcac catagcaaac agaatctgcc
    36721 aggccaggct gaattttata tttttcatca gaataacttc ctcaatggag cgccgttccc
    36781 cgttcagaaa cgcgagaacg tgacatactg ccagggcatg aattgtgttg cgttggtttg
    36841 tagatttttt taacgcttat gatcagtatc atttgcgcgg tatgttgcgc aaccctttaa
    36901 caacagagct tttcgctgca ttaatgcata ataacgcgag tttatggtag gattaatgaa
    36961 taaccatttg ttatagaagg atttttattt gtttaatctg caataatact gattggtgat
    37021 tatttttgat tatatgttca ttcgattatt ttttatgcag ctcaataaca taatcttcat
    37081 caccagatga ttaatctgat ctccgctgca acaatttcgc catattaatc gactcgacca
    37141 gctgtgtccg gttgatccgg ggtgagttga gatccatcac tttttgccat agttcgatgg
    37201 cttgcgcgta attcgcctgc ataaacgcat cggaagccag cagcatcaga gcggtgattt
    37261 cgttagagtc cagcgcgagg gctttgtcga tcattgcgcg agcctggacg gtcatatgtt
    37321 ggctggcctg gtaatacagc accgtcgcca gcgccgcata cagttcagcg ttctcaccac
    37381 gtagttgcaa cgcctgacgg tacgccagca gcgaattgct gtaatcgttt tgccacagat
    37441 aatactcgcc cagtaacgcc cactgttcgc tgttttgtgg attagcacgg attttatcct
    37501 gcaatgcctg aagctgcgct tccggggttt gctggctggc aaactgatgc agcggatcgc
    37561 gctgacgctg atactccgca cgtaccgcct gccattttgg acttaacaga taactgccga
    37621 cacagagaaa taccatcaaa atcgtgagtg ttgtcagcat tttcaccggt atttggggct
    37681 gtttcatcgc ttcgccctca ctcgccagag gatcagtgcc atcagcagta acaacactac
    37741 tgacagcgcc cacaacacca gcgtctgacc cgttaacggt gggttatagc gaacaaaatc
    37801 tccgtagcgt tcggtcatcc agtcaatgat ttcgacttca cttttcccct cttccaccat
    37861 ggtgtaaacc tgatggcgca tactgactgc caccggcgca ttggattcca gtaagttctg
    37921 attctggcac tgcggacaac gtaactggct ggcaatattt aacgcctgtt cctgttgttg
    37981 cggattggcg aattgccagg tgtctacaac ctgagcgcgc gcaaaacagg taaataacag
    38041 cagtagcgtg agaagccctt tattcatcgc gcttccttcc ccgccatccg cttaacaatg
    38101 cgcccgcaat catcaacaat cctccccccc agatccagcg cacaccgctt tgtacataca
    38161 aacgaaaagc gtaacgatcc gctccggttt tctcgcccat gaccgcatac cagtcatgga
    38221 tgccattcca gcgaattgac ggttccatca tttgctgacg acgtgcctca taaaagcgcc
    38281 gctccggcgt taattcacca atgcgttgct gatggtcaaa cagtgccact atcgcttttt
    38341 cgctggtgta attgcctttg gcttgcagat cgaggcgctc aaaacggaag gtgtatcctg
    38401 ccagcgtcac ctgctgaccc ggctgtaaat tcaggctgat ctcctgacgg ctgacgctgg
    38461 aaaccacgat ccccgcggca aataacagca cgcccgcatg cgccagcagc gccggaagct
    38521 gagcacgttt gccagagaca aacgtcgcca gcacaatcac caccagcatc aacagagcaa
    38581 acggtaacgt cgcgcggtta aaatacggcg cgccaacgga gaggcgtccc cagcccagca
    38641 ggccgtaaat catcggataa agcgttccca ccagcacgat cagtagcact gcgcaaaaca
    38701 gcaacagcgt agcgaggatt aacatttccc gcgataaccc cgaaaaacgc accgccgggc
    38761 caccgtcccg cgctcgccag ccatacagag ccagagacgc aaggctaatc agtgcaaaca
    38821 ggctgaacaa cggcacggcg cggacattat ccagcgcgaa cgcatgaacc gaaaccagaa
    38881 tgccagaacg gacaattaag gtgcccagca gcgacagcat cagagtaact atcgccagta
    38941 acagcgacca gtggcggaaa atcccccgct ggcgtgtcaa agacaaactg tgcagcagcg
    39001 cagtggcaga aagccagggt aataaagagg cgttttccac cggatcccag aaccaccagc
    39061 cgccccagcc cagttcgcaa taggcccacc aggaaccgag gatgatcccc gccgttaatg
    39121 cgctccagcc aggtcgtgcc caacgccagc aaattcgggc gctgacacca tcaaactcgc
    39181 tgcgcagtaa actcgccagc gccacgctcg ccgctaccat caaaccgccg tagccgagat
    39241 aaagcagcgg tggatgaaag ataagaccgg gatgttgcag catcggattg agatcgcggc
    39301 cttcgattgc cggtggaaat atccgcacga agggatcgga ccacagcacc acaaacagta
    39361 gcagtgtggc gagcatgaga gataaaacgg ctagcgtcag cggaaatagc ggatcggttt
    39421 gctgtcgata atgccaggca aacagcgcgc tccaggcgga aagcagcagc acccaaagca
    39481 gcagcgaacc ttcatgaccg ccccacaccg ccgccagttt gagttcccac gacagcaggc
    39541 tatagctatg ttgggcgaca taaatgaccg agaaatcgct gatgagaaaa caatacgtca
    39601 gtacgccaaa ggcgagcagc aggagcgcga actgcgccag aatgccgatg caagtgagtc
    39661 gcatcatggc aggccagcgc aaccgcactc ccgcgaaggc cgtcaacggg gtcaacacat
    39721 tgaccccaag acttaacaac agcgccagaa aaccgacttc aggaaggaat acgtccagaa
    39781 accacctctt ttctggctta cgccaccgtc aactgcccgg cgtagagaat gaaaaagcgc
    39841 agcatcaaca cgccgaccag gctggcccca cacgccgcca gcacggcagg aatgccggaa
    39901 ctgcgattga cccacggttt gagcaacatt ggcacaatca gccccagccc ggcgacacca
    39961 agccagaacc accacgtcca gaaaccgccg cccaatgccg ccaccagcgc acgcacttta
    40021 ccgtcatcgc ccagcgccag accgacaaaa aacgccacca gcaggaagat ttcaccccat
    40081 accacgggga tttccatacg gtgtacaaag tgcgcttccg tggaatgcgg gttactgcgt
    40141 tggcgtatcg ccatggcgat cagcgccacc gccgcaccgg acgagatgcc ggagaagagg
    40201 aacagcaccg gcaggatcgg gttattgagg aacggatacg atttcagcgc cgacagcaga
    40261 aagccggtat aagcccccag cagcgccgcc agcaccagca tcaatgtttc cagtcctcga
    40321 tgaacgggcg tcagcaggct cagaaccttt cgcacgatcc ccagcttcgg caaccagcgt
    40381 tgttgcaggg caagcaaatc atgttcaaag attttcgcca gccacagcac cagcaccacc
    40441 atgtagagct gaaacagcat cacccccatc gacatcaccg aggtaaaact gtagtggaac
    40501 atcagcttcc agaaggtcca cggtcttgtc aggtggaaga cgagaatcaa cagaccgagg
    40561 atcaccgcgc ccggcccgac aatcagcgtg gtgcgcagca acgtactgtc tgcaccgcct
    40621 gcctgagggt agaagcgacg taacagtacg gccagcgtca ccagaccggc agaaatacca
    40681 atcaaaaaca ggtagatggc aatcggccag tcccacacca gcgattcaaa atgaaatgcg
    40741 gaagtctgcg tcattggctc acctccccgt atttaaacgg tacgcggtac agcttcggtt
    40801 tggttcccag cgccagcttg tagcggtaag tgggcttctg gcgcagcagt tgcgaaatct
    40861 cactgctggg atcgtccaga ttgccaaacg tcagcgcctt cgtcgggcaa gcctcaacgc
    40921 acgcgggcag cttaccggcc tgcaaatggg tcttacggca gaaatcgcat ttgtccgccg
    40981 tcttcgtgac cggatggata aagcgcacgc ggtacggaca ggcggcgatg cagtactgac
    41041 aaccgacgca gagatccgga ttaacatcga caatcccact ggcagcgtcg cgaaaagacg
    41101 ctccggtcgg gcagacgtca acgcacggcg catgatcgca gtgctggcaa gacttacgga
    41161 agaagcgata tttcacatca ggaaattcgc cctgcggctc gctacgaata atcgtcaggc
    41221 gcgagacgcc ttccggcacc ttgttcactt tccgacaagc atccatacag gcggtacagc
    41281 cgatgcataa cgactcgtca tgcaccatgc cgtaacgcac cccattgata ttcaacgttt
    41341 tcgccaccac gcgccctgcg gtgccgctga cggcggccag cacaccgacg ccggtgagaa
    41401 actggcgacg tgaccaggtc atggctgctc cttaagcaac ggaacagacg ccgggttaaa
    41461 gttcggattg gtgcgttgat cgctgtggca atcgacgcaa atcttaatcc gtcctttgtc
    41521 acttaacgtc tgcatcgtat cttgctgtgg atgcagggaa tggcagctgg cgcacgccac
    41581 tttggttacg tggacatcgt gcggccagaa cgctttttgc aactgttccg gcagatggca
    41641 ggacatacaa acactgttct gctctccaac cttgtacatc ggctcgttaa agcgcatcac
    41701 atctttcacc ccttcgcggt gttgcggtga tggctgaccg tggcagttgg tgcaggtgac
    41761 cggcagtttg ttattcgggt tgataacaga agcatgtttg ccatgcatac cttcggtatc
    41821 tggcttatga cagtccagac aggcggcatc cgggttacgc tgctgggtaa cttcgtagcg
    41881 atcatcagat gcctgcgccg caggggtagc ggtaattccg ttcaggctcc acaacaggcc
    41941 cgacgccagc accccggcag ttaataacga acgtaatacg ctcatattca ctccattgtg
    42001 ttttccgccc cttgcgaggc ggaacggggt tattggctta acagaccgtt tttacgtgcc
    42061 tgctcttccc actgcgggat caccgttttg atgaagtcct gcttctcggc cttgatttgc
    42121 tccatgttca ggccaatggc ctgctgggct ttctctttgg tcgagatatc cgggatctgg
    42181 atttcatggg taatgccttt ggtcgccagc aggcgcgcca gtttggtgcg tgcatccgcc
    42241 gctttatcca tcgccgtacc gagcatcctc agaccttctt ccggtgcgtg catatgaatg
    42301 ccgtgggaag cgatcgccaa atcccaacgc cactgggcat gacggatatc gtcctgaatc
    42361 ggcttcattt cggcttccgt tgcgcctgca tccagcgccg ctttcgcttc gaagtgagcg
    42421 tgaaccagtt gatcttcaac cttgattttc aggtcgttaa tcgactgctt acgttccgcg
    42481 accacttttt gcaaggcagc tttgtcctgg gtatggcagt tcgcacaagt ctgggcgaag
    42541 ttatcaaacg gattaccaat tttatggtcg gtgtagagtt tgccttcggc gttctgcact
    42601 tttggcatat ggcaatcgat acaggtcacg ttgtttttgc cgtgaatgcc cgctgtccag
    42661 gtttcatatt ccgggtgctg cgctttcagc attggcgttt tcgacaggga gttagtccag
    42721 tcagagaagg caattttgtc gtaatactgc tccatatttt cgactttcat gccgtcatcc
    42781 cacgggaatt taaccgcttt gtttttgccg tcgaagtaat actccacatg gcactgaccg
    42841 caaaccatcg attgctggtc gaaacgtccg gctttctcga aaggtttacc aatggcttcc
    42901 atcgcgcgag ccgcatacgg acgggaaagg gttaactccg gtttgccttt ggcgaactct
    42961 ggtgaggcgg tgttatggca atcggcacaa cctaagttgt tgacgatttc cgggccgccg
    43021 cgcgcccatt ttccgtggaa gtagccatct tcgccgtctt tctggatcag acgcgccaca
    43081 tccgggcttt tacaactcca gcacgccatc ggtagcggac catcttcagc gtttttcggc
    43141 gcaccggtac gcagggtttc acgcacatcg gtcacagcaa aggcatgtcc acgcggcttg
    43201 ttgtaatcgc gcgagaaggg ataccctgcc cacaggatca ccagccgtgg atcttccgcc
    43261 agggcgtcaa cacgctctga ctgttccgag gtagctttcc aggagagata ttgatcggga
    43321 tgctgcgggg caaaggtttc attcttcgct tccacagtta caggtgttgc gggagcagcc
    43381 gtttgttcag cgtgaacaga agtgaaaaag aaaaaaggaa tcaataagct gaagatacgg
    43441 cgtgcgttta tttttatcct tgtcataggg cttcatccga attgcgccat tgttgcaatg
    43501 gcggttttta ttgtttttca cgacagtaac cgcacctaca ctgtcatgac attgctcgtc
    43561 cctatgtgta acaaataacc acactatgaa tgttgtcttt aatcaattgt aagtgcatgt
    43621 aaaataccac tttagagtta gtcagtatct tcctcttttt caacagcatg cataactgca
    43681 tgttcctcaa agaattaatc aacttttgtt gctgaccttc aaaaattacc ctgccgttta
    43741 tttgcacaat tctacttttg cgtgatctgt cgcccaaata ctaaacaaaa ctgccaatac
    43801 ccctacattt aacgcttatg ccacatatta ttaacatctt acaaggagaa caaaagcatg
    43861 agtcaaattc acaaacacac cattcctgcc aacatcgcag accgttgcct gataaaccct
    43921 cagcagtacg aggcgatgta tcaacaatct attaacgcac cagatacctt ctggggcgaa
    43981 cagggaaaaa ttctcgactg gatcacaccg taccagaagg tgaaaaacac ctcctttgcc
    44041 cccggtaatg tgtccattca atggtacgag gacggcacgc tgaatctggc ggcaaactgc
    44101 cttgaccgcc atctgcaaga aaacggcgat cgtaccgcca tcatctggga aggcgacgac
    44161 gccagccaga gcaaacatat tagctataaa gagctgcacc gcgacgtctg ccgcttcgcc
    44221 aataccctgc tcgaactggg cattaaaaaa ggtgacgtag ttgcgattta tatgccgatg
    44281 gtaccggaag ccgcggtcgc gatgctggcc tgtgcccgca ttggcgcggt acattcggtg
    44341 attttcggcg gcttctcgcc ggaagccgtt gccggacgca ttatcgactc cagttcacga
    44401 ctggtgatca cttccgacga aggtgtgcgt gccgggcgta gtattccgct gaagaaaaac
    44461 gttgatgacg cgctgaaaaa cccgaacgtc accagcgtag agcatgtggt ggtactgaag
    44521 cgtactggtg gaaaaattga ctggcaggaa gggcgcgacc tgtggtggca caacctggtt
    44581 gagcaagcca gcgctcagca ccaggcggaa gagatgaacg ccgaagatcc gctgtttatt
    44641 ctctatacct ccggttctac cggtaagcca aaaggcgtgc tgcacactac cggcggttat
    44701 ctggtgtacg cggcgctgac ctttaaatat gtctttgatt atcatccggg cgatatctac
    44761 tggtgcaccg ccgatgtggg ctgggttacc ggacacagtt atttgctgta tggcccgctg
    44821 gcctgcggcg cgaccacgct gatgtttgaa ggcgtaccga actggccgac gcctgcccgt
    44881 atggcgcagg tggtggacaa gcatcaggtc aatattctct ataccgcgcc cacggcgatt
    44941 cgcgcgctga tggcggaagg cgataaagcg atcgaaggca ccgaccgttc gtcgctgcgc
    45001 attctcggtt ccgtgggcga gccaattaac ccggaagcgt gggagtggta ctggaagaag
    45061 atcggcaacg agaaatgccc agtggtcgac acctggtggc agaccgaaac tggcggtttc
    45121 atgatcacgc cactgcctgg cgctaccgag ctgaaagccg gttcggcaac acgtccgttc
    45181 ttcggcgtgc aaccggcgct ggtcgataac gaaggtaacc cgctggaggg ggccaccgaa
    45241 ggcagtctgg tgatcaccga ctcctggccg ggtcaggcgc gtacgctgtt tggcgatcac
    45301 gaacgttttg agcagaccta cttctccacc ttcaaaaata tgtatttcag cggcgacggc
    45361 gcgcgtcgcg atgaagatgg ctattactgg atcaccgggc gcgtggacga cgtgctgaac
    45421 gtctccggtc accgcctggg gacggcagag attgagtcgg cgctggtggc gcatccgaaa
    45481 attgccgaag ccgctgtggt cggtattccg cacaatatta aaggtcaggc gatctacgcc
    45541 tacgtcacgc ttaatcacgg ggaggaaccg tcaccagaac tgtacgcaga agtccgcaac
    45601 tgggtgcgta aagagattgg cccgctggcg acgccagacg tgctgcactg gaccgactcc
    45661 ctgcctaaaa cccgctccgg caaaattatg cgccgtattc tgcgcaaaat tgcggcgggc
    45721 gataccagca acctgggcga tacctcgacg cttgccgatc ctggcgtagt cgagaagctg
    45781 cttgaagaga agcaggccat cgcgatgcca tcgtaaccca caattgccgg atgcgctccg
    45841 ggcaacaagt cggaaacgat ttgtaggcct gataagacgc gacagcgtcg catcaggcat
    45901 tgattgccgg atgcggcgtg aatgccttat ccggcctaca ttcggcaagg gttacccaag
    45961 cgttaacctt ctcccataag ggagcgggaa ttaaaacaat ccctacatta cctctggaga
    46021 atctgtgatg aatggtacga tttatcagcg gatagaagac aatgcgcatt tcagggagtt
    46081 agtcgaaaaa cggcaacggt ttgccaccat cctgtcgatt attatgctgg cagtttatat
    46141 cggctttatt ttactgatcg ccttcgcgcc cggctggctg ggcaccccgc tgaatccgaa
    46201 caccagcgtc acacgcggta ttccagtcgg tgtcggggta attgtgatct cctttgttct
    46261 cactggtatc tacatctggc gggcgaacgg cgaattcgac cgtcttaata acgaagtcct
    46321 gcatgaggta caagcatcat gaaaagagtt ctgacggcgc ttgccgccac actccctttc
    46381 gcagctaacg ccgcggatgc tattagcggg gccgtagagc gccagccaac gaactggcag
    46441 gcgattatta tgttcctgat tttcgtcgtg tttacgctcg gcattaccta ctgggcgtca
    46501 aaatgcgtac gttcgcgtaa tgactactac accgcaggcg gcaatatcac cggttttcag
    46561 aacgggctgg cgattgccgg ggactatatg tccgccgcct cattcttggg gatctccgca
    46621 ctggtgttta cctccggcta tgacggctta atttactcgc taggcttcct ggtaggctgg
    46681 ccgatcattt tgttcctgat tgccgaacgt ctgcgtaacc tcggtcgcta cacctttgcc
    46741 gatgtggcct cttaccgtct gaaacagggg ccgattcgta ttctttcggc ctgtggttct
    46801 ctggtggtgg tggcgcttta ccttatcgcc cagatggtgg gcgcaggtaa actgatcgag
    46861 ctgctgtttg gccttaacta tcacattgcg gtagtgctgg tcggcgtgct gatgatgatg
    46921 tacgtcctgt tcggcggcat gctggcgacc acctgggtac agattatcaa agccgtgctg
    46981 ttgctgttcg gtgccagctt tatggccttt atggtgatga aacacgtcgg ctttagcttc
    47041 aacaatctgt tcagcgaagc gatggcggta cacccgaaag gtgtcgacat catgaagccg
    47101 ggcgggctgg tgaaagatcc tatctccgcg ctctctttgg ggctaggact gatgtttggt
    47161 acggcgggct tgccgcatat tctgatgcgc ttctttacgg tcagcgatgc ccgcgaagca
    47221 cgtaagagcg tgttctacgc caccggattt atgggctact tctacatcct gacctttatt
    47281 atcggctttg gcgcgatcat gctggtcggt gcgaatccgg aatataaaga cgcggcgggc
    47341 catctgattg gtggtaacaa catggcagca gttcacctgg ccaatgcggt gggcggcaac
    47401 ctgttcctcg gctttatttc agcggttgct ttcgccacca ttcttgcggt ggttgcgggt
    47461 ctgacgctgg cgggcgcatc ggcggtgtct cacgacctgt acgctaacgt cttcaaaaaa
    47521 ggtgcgaccg aacgtgaaga gctgcgggta tcgaaaatca ccgtactgat cctcggtgtg
    47581 attgccatta tcctcggcgt gctgtttgag aatcagaaca tcgcctttat ggtggggctg
    47641 gcgtttgcca tcgcggcgag ctgtaacttc ccgatcattc tgctttccat gtactggtcg
    47701 aaactgacta cccgtggcgc aatgctgggc ggctggttag gactgattac tgcggtggtg
    47761 ctgatgatcc tcggcccgac gatttgggta cagatcctcg gtcacgaaaa agccatcttc
    47821 ccgtatgaat acccggcgct gttctctatc agcgtggcat tcctcggtat ctggttcttc
    47881 tcggcaacgg ataattcagc ggaaggtgcg cgcgagcgtg aactgttccg tgcgcagttt
    47941 atccgctccc agactggctt tggcgttgag cagggccgcg cgcattaatc tctccccttc
    48001 cccggtcgct tgatcgggga atttcttcag aacatcatcc tggccaccag cggcgaaacc
    48061 aataccatca atacacctga aagcatcatg accaggcttg caactacgcc ttcttgttga
    48121 cccagttcat acgaccgcgc agtcccggca ccatgagatg ccgcgccgaa tccagcacct
    48181 ttcgccatcc cttcgcgaat agagagacgt aaaaacaaca cgtcgccaat cgccatgcca
    48241 aacacaccgg tgaccaccac aaacagcgcc accagatcag gctgtccgcc gagcgactgg
    48301 ctcgctgcca aagcaaatgg cgtagtgacc gaacgcaccg ccaggctacg ctggatctca
    48361 tctggcaggg taaacaatcg cgccagccac actgagctgg tcactgccac caccgttgcc
    48421 gtcagtacgc ctgccgtcaa cgacatccag tgacgcttaa tgatcgcccg attgtcatac
    48481 actggtaccg caaaggcgat ggtggccgga cctaatagcc atagtaacca gtgcgcttcg
    48541 ccaatatagt tttgccagga aatattgcca aaaaccagca gcataaccag tagcgtaggc
    48601 gtcagcacca gaggcatcaa tggcaattta cgaaagcggc gataaaggcg tttgttaaga
    48661 aaatagataa ctagcgtaat tgccagacag agcatactta gctgaaaatt agccacggtt
    48721 taacctgcgc atttcatagc gatagacttt ttccactacc caggcagtcg tgcccagcac
    48781 catcaccgta ctcagcgcga tcacagcaaa aatacgccag ccatccacca gtaatagttg
    48841 tgcatagttc accacagcca ccacggcagg gacaaaaaac agcaacattt cggccaatag
    48901 ccaacgcgcc ccggctcgaa cccattgcag aggaagaatg cgacaaacaa tcagcgccag
    48961 catcaggatc atccccacca ggttggcagg cagcggcaaa tgcagccagg agacgagata
    49021 ctgcgcaaaa ataaataaac ctgcatacag cacaacctga aaaaatacct ggatagattg
    49081 cactacggca ggggtaatgc ggcgaagcgc cacgggcatg agaggatatc ctgaaaataa
    49141 aacagaatgg taagtataga gagcgctcgc ccctgactga aatgaattaa aatcattgca
    49201 ggtatagtca aagggaatag ttatggatat cagaacgctg cgttattttg tcgaagtcgt
    49261 gcgccagcaa agcttcaccc gcgcagcgga gaagttgttt gtcacccaac caactatcag
    49321 caagatgctg aagaatctcg aagatgagct gaactgtata ttgttgatcc gcgatggtcg
    49381 tcggctgctt ctcactgata ctggtcgcgt cgtatttgag cgcgggctgg ctttactggc
    49441 tgagtttcgg caactggaag ctgagcttag cgatatcaat cagcttaaaa ccggcgtttt
    49501 gcggctggga atcccgccga tggttgggat gttgatggcc gggccgattg gcctttttcg
    49561 ccagcgttac cccggcgtag aattgaaaat ttcggagttt ggtggtctga cggtacaaca
    49621 agcggtgatg aacggtgaac tggatatggc actgacagcc ctgccagtag aagaaagcgg
    49681 cctgacaacg ttatcgctgt ttagtcatcc actatgtgta ctgacgccac attcaggtaa
    49741 atggcttgaa tgtgatgccg tctcaccgga gacattggcg gaacatcctc tacttatcta
    49801 taacgaagat tttgccttaa gtaagcaatt gatgaagtta ttcgatgaac atggcgttaa
    49861 gccgagaatt gcagttcgca gcgggcaatg ggattttctc gccgcgatgg ttcaggccgg
    49921 tgtcggaata gccatattgc cggaacccat ttgccagcga ttagataaaa acaccttaaa
    49981 atggctaccg ctggaaagcg aactaagctg gaaattagga atgatttggc gtgagggcgt
    50041 ttacctttcc cactctgccc acgcgtggct gagctgttgt gaaggatact ggttaacgcc
    50101 agagtaagtc gggttggcgg gctcgcccgc caaccatgca gattactgat tttcctcaat
    50161 cagcaacgct tcaagcaaat cgagatcgtg cagtaatttt tgcagcgttt cgttactgat
    50221 ctcccgcgtg gcgcgcaggt ggtacagttc agcacgttca gaacgcaatg ccgccagacg
    50281 gaagcgacgc tccaggttct cttccaggat tgaactttct acatcattac gtccatcggc
    50341 gcgacgacgc aggttaccaa tgacgcgaga actaacctct gtgagcagct ggttatcgat
    50401 attctcttcg gtatccgccg ccagacgctc ctccattttc tggatcgcca caatcgccac
    50461 ttctgccgtt gccgctcgtg caatacgttc ctctttcaat tgttgcgaat ggtccgcgac
    50521 ttctatgtgt tgtagcagaa taggcaacat caccacgccg acaaacaatg agaagagaat
    50581 gacgccagcc gccaggaaca ccaactcata gcgcgccggg aagacgttac catccggcaa
    50641 gagaagcggg atagagagca caccggccag agtgatcgcc ccacgcaccc cggcgaaaga
    50701 cgcgatcagg atttctcgtg tagtccacga accaaactcc atcggcttct tcttcaggaa
    50761 gcggttgctg aactttttca tcgtccacag ccagccgaaa cggaccagca tcagcgccgc
    50821 atatatcaga ataatatcgg taaacagcat ccagatttcg acgttagggt cgatttctgc
    50881 cgccatcagc gacgtctcca gaatccccgg caactgcaga cctaacagca ggaacaccat
    50941 gccgttaaag acaaattcca gcatcgccca ggtgctgttt gcgcgcaggc gcattgccag
    51001 cggcgcacgg cgcatcacac cggagcgggt gatggtcatc ccggcggcaa ccgcagcgag
    51061 gatgccggaa acgccaatat gttccgcaat cagataagaa gcgaatggca gcaagaacag
    51121 cagaacgatc tgcgtcgcgg gttcatcacc gccccagcgg ctgaggaatc gcagcgagcg
    51181 accatacagc cagctcacca caaaaccggc gagaataccg cctattgcca ctttcataaa
    51241 ttcgaccgtc gcaccgccga cggtgaagat cattgtcccc atcgccactg ccacggcaaa
    51301 cttcaacgac accaggccgg aggcgtcatt catcaacgct tcgccctgca aaatgcccat
    51361 gatttttttc gggatgcgcc cctcgccaac aatcccggag agcgctacag catcggtcgg
    51421 agaaagcacc gcagccagcg caaaggcggg gatcagcgga atgcccggca ccacccagta
    51481 aataaggaag ccaatgccga ccacggtgac caccaccagc gccagcgcga ggccgaaaat
    51541 ctctcgccca tgttcgagaa attcacgggt cggcgttttc cagccatcag cgaacagcaa
    51601 cggcgggata aacaagacta aaaagagttc aggatcaaac tccacatgca aaccaaacgt
    51661 cggccacgcc agtagcgcac cgatggcgat ttgcataagc ggaagcggga tctgaaaggg
    51721 catgacacga gtgaccaccc cggacagcga gaccacgagg gtcattatca gtatggtgaa
    51781 gaagatttcc atgcgttccc tgtttgcgat gttttttatg atctgagaaa ggcgttatgc
    51841 ctgaattctc attttttcag aatatcgcaa aagctcacac aatgtgcgga gaaaataaaa
    51901 acgggcagcc attgccaccc gttttcagaa tcctatgtaa gattagatag cccagccacc
    51961 cgcatagaag gtcaccagcg ccacggcaat aacgaccgta ccgatgttca acttgcgcca
    52021 ttcgccggaa accagacgac caatcaccag agtcgcgaag ccgatcatga tgcctgttac
    52081 gatgttacag gtcagcacga tgaataccgc cgtaaccaga cccgccatcg catcaacaaa
    52141 atcagcaaag tcgattttcg ccacgttgct cagcatcagc aggccaacgt acatcagcgc
    52201 cggagccgtt gcgtaccccg gaacgaggta agagagcgga gagaggaaca gaatcagcag
    52261 gaacagcacg ccaacggtga tagccgtcaa accggtttta ccgcccgccg ccgtacccgc
    52321 cgcagactcg atgtataccg ctgccggagc tgcacccacc aggccagaaa aaacgctgct
    52381 catggagtcg gtggtcagtg ctttcccgcc gtcgatgatc tgcccatctt tatccagcag
    52441 gttcgcctga ccggcgacgg cacggatagt tccggtggca tcaaatactg ctgtcatcac
    52501 cagtgccaga acacttggca ggactacagg attcagcgcg cccataatgt ccaggctgcc
    52561 aatcagtgaa ttgccgtttt catcgctcaa tgaaggcatg gcaaaaacgc cggagaaatg
    52621 gacgttagga tcgaagatca aaccgacaat tgagataccg ataatggtca gcagaatgcc
    52681 accagggact ttcagttttt ccaggccgat aatcactgcc agacccacca acgacatcat
    52741 caccggaaag gtcgtgaaat cccccagcgc gacgggcaga ccatcaagtg ggtttttaat
    52801 caccagaccg acaccattag cagcaatcag cagcaggaac agaccgatac caatccccgt
    52861 gccgtgcgcc acaccgtgcg gcaagttgcg caaaatccag ctacggatac ccgtggcaga
    52921 aattaccgta aacagcacac ccatcaggaa cacggcaccc agcgcgacag gtacgctaat
    52981 atgttgcccc agcaccaaac tgaatgcggt aaacgccgtc agagagatgg cgcaaccaat
    53041 cgccaacggc agattagccc acagacccat cacgatagaa ccgagtccgg caaccagaca
    53101 ggttgcaacg aaaactgccg caggcgggaa gcccgcttta cccaacatgc ctggaacgac
    53161 gataactgag tagaccatcg ccagaaacgt tgttaacccg gcaaccactt cctgacggac
    53221 agtgcttcca cgttgtgaaa ttttaaacca ggcgtcgagt gaaccgccgg tacgcgctga
    53281 tggcgtagac ataagaaaac atcccctgag agtttaattt tcgtcaagat gcgtggcgga
    53341 caatcccctg ccagcgaaac gttatcttcc tgctccaggt ttgcgacaat ttgctgcgtc
    53401 gcaaaaaaag caaacgttta gctccgcgca tacaaaacgg gtggtaaaaa aagcaaacga
    53461 ttatccggcg atcatagcgg ctttttcaac cgaagtttgc tgcttttttc tcttttattg
    53521 tcttgacgat gaaaaaccag gcaattgatg cgcaaacgtt atcgtcgatg cgcaagattg
    53581 cagcgttatc gagccgtata ccgttcagga ttgcgataaa aaatcgtctg agatgacaaa
    53641 aatattgcgg ctaaaaagtg tacttttaca ctggcgaaga aacgccggtc acatacaatt
    53701 aaagcatcaa caccaaccgg aacctccacc acgtgctcga atgaggtgtg ttgacgtcgg
    53761 gggaaaccct cctgtgtacc agcgggatag agagaaagac aaagaccgga aaacaaacta
    53821 aagcgccctt gtggcgcttt agttttgttc atcttccagt aagcgtgcgc cggtaccttc
    53881 ttctcctaat cggtcgcccg ggttacgcaa cgggcaatca ctgcgcgaaa ggcagccaca
    53941 accaatacat ccatccagtt cgtcacgcag cgccactaag gtatgaatgc gccgatccaa
    54001 ctcttctcgc cattgggacg aaagctgttt ccactctttc gcacttaacg tatgcccttc
    54061 tggcaacacg ccaaacgctt cacgaatggt tgcgagcgga atgccaatac gctgagcaat
    54121 tttaataatt gcgacatatc gcaacacatc acgtttatat cgccgctgat tgccgctatt
    54181 acggatactg gtaatcaacc ctttgctttc atagaaatgc agcgccgata ccgccacacc
    54241 gctgcgtttc gccacttcgc cgggggttag cagcgcttta atgcggggta atttcttttc
    54301 cataaatcgc tttacctcaa gttaacttga ggaattatac tccccaacag atgaattaac
    54361 gaactgaaca ctgaaaagag gcagatttat gtcccatcag aaaattattc aggatcttat
    54421 cgcatggatt gacgagcata ttgaccagcc gcttaacatt gatgtcgtcg caaaaaaatc
    54481 tggctattca aagtggtact tgcaacggat gttccgcacg gtgacgcatc agacgcttgg
    54541 cgattacatt cgccagcgcc gtctgttact ggccgccgtt gagttgcgca ccaccgagcg
    54601 tccgattttt gatatcgcaa tggacctggg ttatgtctct cagcagacct tctcccgcgt
    54661 tttccgtcgg cagtttgatc gcactcccag cgattatcgc caccgcctgt agttttattg
    54721 cccgcgcgtt aactcccgcg cgggtaatta ccccatccat tgcataaaca cctgcggcgg
    54781 catcgccttc gcaaaaaacc atccctggca atagcgcacg ccgcgtttgc gcagccagtt
    54841 aacctgcgcc tcagtttcga caccttcagc gatcgttttt aaccccaggc tgtgcgccag
    54901 ctcgatgatg tgttccgcaa tcaaatgact ggttttgtgg gtggtcagcg tctcaacaaa
    54961 cgatttatcg attttcaaaa tatcgacatt caatgattta aggttatgca agttagagta
    55021 gccaatacca aaatcatcaa ttgccacttc gtaaccggcc ttgcggaaag ccagaataat
    55081 cggcgtcatt ttgtcgacat caagaaatgc atgttcagtc acttcaaatt taatttgctg
    55141 cgggcgcacc gcatgttgct ctgttttctg attggttcgc gctatcaacc gtgaggtatg
    55201 aaaatcggag gctgacaggt taatagaaac atagcgatcg gtatgtgttg ccagatacgc
    55261 gcccaaatca cggaagacat tatcaataac ataatcagtt acctgttcga tcatcccctc
    55321 cttttctgcc agcggaataa actctgccgg attcattact tgcccctgct caccaggcca
    55381 acgtaacaac gcttcagcgc cgatacattt ttctgttttg atatcgatta ttggctggta
    55441 ataaaggcaa agctgatgtt tttcgagggc gcgttgcaat ttgcgtttgg gagacaaata
    55501 gttttgtcga atacgtagcc agagtaatag cagaacaaga ctccccaaaa taccggcggg
    55561 taacgcaaaa ataagatgat tataaaaatg ggttataaga cgttgatatg aagtcgcaac
    55621 aatggctgca attgggcgtt ttgtcgaata aactgtcgca tataaatagc catttcgttg
    55681 tacggttaaa tcattcaaat gaatcagcgg agaaaacgtt gctgccgagg cctcgttgct
    55741 taacgagaaa aaggttttcg tcaccgtatc atacacaccc cattgcaatg tcggatcatc
    55801 agacatcact tcactccaga agagagggtt gataaccgcc acataatttc cccgctgcat
    55861 gtaggtcatt ttatagccag agaaaaaagg cgtatcacgg taataataga tagaaacgtt
    55921 aggttcacgc ttataatcgg ccggtgcaat cgtatagcca tttacagacg ctatcagcga
    55981 tgagcataaa aaatggttat cacgagcata aatcaattca ttaatataaa gatagccacg
    56041 aataatattc aacattcgct tttgatgggc tggagtgcaa acttgcccct gatagcgttc
    56101 agctgcatcg ctcactaaat ctgcctgacg aatgaccagc tcagatttat ccaacgccag
    56161 ctgagcaaaa gttcggagct gattattcac ttctgatttt gcccaaaaga aggcaatcca
    56221 tagcgacaga atgatgggaa agagaactaa aaagataatg cccggaaacg ccagtaattg
    56281 gtgtcgtgca cgatgactca ttgctatcaa ttccctgcct gtttataccc ggttattgca
    56341 aggtgaaacc ctggctaccg acgtctttag ataaatctta cggtataaat agcacttttg
    56401 cctgatataa aattgagacc tttttaatcg tcaccaggat gacgataatt cattgatctt
    56461 aaaaattaat caccttcagg aatgcagagg cggcgggaag atataccctt aatgccgctg
    56521 aaaaaacaca taatctgtac ggcaataaaa gtcacggcct aatctggcta acaaacaacc
    56581 aattgtgata tagttcacaa aattcatgaa acaaacagag tgtttcattt ttgtgttctg
    56641 caagcccccg cgatagccag agtgtatcac gcctcccgtg aacaacgccg cgctgtccag
    56701 ggcatcagct cttttccgag gaattgtttg atggctaccc tcaccactgg cgtggttctt
    56761 cttcgctggc aacttcttag tgccgtaatg atgtttctgg ctagcacgct caacatccgt
    56821 tttcgtcggg ctgattatgt cgggcttgca gtgatcagca gcggtctggg cgtagtttcc
    56881 gcctgctggt tcgcaatggg gttgcttggc atcacaatgg cggatatcac cgccatctgg
    56941 cacaacatcg agtcggtgat gatagaagag atgaatcaga caccgccaca atggccaatg
    57001 attttgactt gatatgtaga agcctccata acggaggctt cttttttatg gctggcagat
    57061 gctttaatca tccaccttaa aacaatataa cctattgttt taaagacaaa tcagaacgga
    57121 atatcgtcat caaagtccat cggcggctcg ttagacggcg ctgccggagc ggactgctgc
    57181 gggcgagact gcgcgccgcc gctgaactga ttgccaccct gcggctgctg aggctgaccc
    57241 caaccgccct gcggctgacc accaccgata ttgccacctg caggagcgcc accaccctga
    57301 cgaccaccca acatctgcat ggtgccgcca acgttcacca cgacttctgt ggtatagcga
    57361 tcctgaccgg attgatcggt ccatttgcgg gtacgcagct ggccttcgat ataaacctga
    57421 gaacctttac gcagatattc gctggccact tctgccagtt tgccgaacag cacaacgcgg
    57481 tgccattcag tctgctcttt catctcgccg gtcgctttat cacgccagga ttcggaagta
    57541 gccagcgtaa tgttggcaac tgcgccacca tttggcatgt agcgtacttc cgggtcctga
    57601 cccagattac caacgagaat aaccttgttt acgcctctgc tggccatgtt cgtgtctcct
    57661 gaaaaaaaat cgttctgaat aagtgtaaac gcgcgattgt accattacca atagcgcttt
    57721 tactatgttg tgacctcggt tccgggaaac aaacctggct agacattgtt acacaacact
    57781 ccgggtaatg cattccaata ctgtatattc attcaggtca atttgtgtca taattaaccg
    57841 tttgtgatcg ccggtagcac catgccaccg ggcaaaaaag cgtttaatcc gggaaaggtg
    57901 aatggataag atcgaagttc ggggcgcccg cacccataat ctcaaaaaca tcaacctcgt
    57961 tatcccccgc gacaagctca ttgtcgtgac cgggctttcg ggttctggca aatcctcgct
    58021 cgctttcgac accttatatg ccgaagggca gcgccgttac gttgaatccc tttccgccta
    58081 cgcgcggcag tttctgtcac tgatggagaa gccggacgtc gaccatattg aggggctttc
    58141 tcctgccatc tcaattgagc agaaatcgac gtctcataac ccgcgctcta cggtggggac
    58201 aatcaccgaa atccacgact atttgcgtct gctgtacgct cgcgttggtg agccgcgctg
    58261 cccggaccac gatgttccgc tggcggcgca aaccgtcagc cagatggtgg ataacgtgct
    58321 ttcgcagccg gaaggcaagc gtctgatgct gctcgcgcca atcattaaag agcgcaaagg
    58381 cgaacacacc aaaacgctgg agaacctggc aagccagggt tacatccgtg ctcgtattga
    58441 tggcgaagtc tgcgatcttt ccgatccgcc gaaactggaa ctgcaaaaga aacataccat
    58501 tgaagtggtg gttgatcgct tcaaagtgcg tgacgatctt acccaacgtc ttgccgagtc
    58561 gtttgaaacc gcgctggagc tttccggtgg taccgcggta gttgcggata tggacgatcc
    58621 aaaagcggaa gagctgctat tttccgccaa cttcgcctgc ccaatttgcg gctacagtat
    58681 gcgtgaactg gaaccacgcc tgttttcgtt taacaacccg gcgggtgcct gcccgacctg
    58741 tgacggcctt ggcgtacagc aatatttcga tcctgaccgc gtgatccaga atccggaatt
    58801 gtcgctggct ggcggtgcga tccgtggctg ggatcgccgc aacttctatt atttccagat
    58861 gctgaaatcg ctggcagatc actataagtt cgacgtcgaa gcgccgtggg gtagcctgag
    58921 cgcgaacgtg cataaagtgg tgttgtacgg ttctggcaaa gaaaacattg aattcaaata
    58981 catgaacgat cgtggcgata cctccattcg tcgtcatccg ttcgaaggcg tgctgcacaa
    59041 tatggagcgc cgttataaag agacggaatc cagcgcggta cgtgaagaat tagccaagtt
    59101 tatcagcaat cgtccgtgcg ccagctgcga aggaacccgt ctgcgtcggg aagcacgcca
    59161 cgtgtatgtc gagaatacgc cgctgcccgc catctccgac atgagcatcg gtcatgcgat
    59221 ggaattcttc aacaatctca aactcgcagg tcagcgggcg aagattgcag aaaaaatcct
    59281 taaagagatc ggcgatcgtc tgaaattcct cgttaacgtc ggcctgaatt acctgacact
    59341 ttcccgctca gcagagacac tttccggcgg tgaagcccag cgtattcgtc tggcgagcca
    59401 gattggtgca ggcctggttg gcgtgatgta cgtgctggat gagccgtcta tcggcctgca
    59461 ccagcgcgat aacgagcgcc tgctgggtac gcttatccat ctgcgcgatc tcggtaatac
    59521 cgtgattgtg gtggagcacg acgaagacgc gattcgcgcc gctgatcatg tgatcgatat
    59581 cggtccgggt gcgggtgtac acggcggtga agtcgtcgcg gaaggtccgc tggaagcaat
    59641 aatggcggtg ccagagtcgt tgaccgggca gtacatgagc ggtaaacgca agattgaagt
    59701 gccgaagaaa cgcgtacccg ccaatccaga aaaagtgctg aaactgacgg gcgcacgcgg
    59761 caacaacctg aaagacgtga cgctgacgct gccggtaggt ctgtttacct gcatcacagg
    59821 ggtttcaggt tccggtaaat cgacgctgat taacgacaca ctgttcccga ttgcccaacg
    59881 ccagttgaat ggtgcgacca tcgccgaacc ggcaccgtat cgcgatattc agggactgga
    59941 acatttcgat aaagtgatcg atatcgacca aagcccaatt ggtcgtactc cgcgttctaa
    60001 cccggcaacc tacaccggtg tgtttacgcc tgtgcgcgaa ctgtttgcgg gcgtaccgga
    60061 atcccgtgcg cgcggctata cgccaggacg tttcagcttt aacgtccgtg gcggacgctg
    60121 cgaggcctgt cagggcgacg gcgtgatcaa agtggagatg cacttcctgc cggacattta
    60181 cgtaccgtgc gaccagtgca aaggcaaacg ctataaccgt gaaacgctgg agattaagta
    60241 caaaggcaaa accatccacg aagtgctgga tatgaccatc gaagaggcgc gtgagttctt
    60301 tgatgcggtg ccagctctgg cgcgtaagct gcaaacgctg atggatgttg gcctgacgta
    60361 cattcgcctc gggcagtccg caaccacact ttctggtggt gaagcccagc gcgtgaagct
    60421 ggcgcgtgag ctgtcaaaac gcggcaccgg gcagacgctg tatattctcg acgagccgac
    60481 caccggtctg cacttcgccg atattcagca actgctcgac gtactgcata aactgcgcga
    60541 tcagggcaat accattgtgg taattgagca caatctcgac gtgatcaaaa ccgctgactg
    60601 gattgtcgac ctgggaccgg aaggcggcag tgggggcggc gaaatcctcg tctccggtac
    60661 gccagaaacc gtcgcagagt gcgaagcttc gcatacggcg cgcttcctca agccgatgct
    60721 gtaatcgtta aggccgcttt ttgagcggcc tcagattatt gacaaagtgc gctttgttct
    60781 tgccggatgc ggcatgaacg ccttatccgg cctacaaaaa catgcaattt caatatattg
    60841 cagagattac gtaggcctga taaagcgtag cgcatcaggc aattttgcgt ttgtcatcat
    60901 tctcaggccg ctttctgaac ggtcttttcc tttcagagtt gcaccagtaa tttacgtttt
    60961 tcttccggca gcaaattcac cgcctgctga taagacgcat ccaccagata atagatttgc
    61021 gaatccggca gcgaaccgtc gagatacacg gtgctccagt gcgctttatt cagatggcgg
    61081 cttggacgca catcgctgtg ctgctgacgt agcagctccg ccagctccgg gctggttttc
    61141 agcgaaacag ctgggcgatt ttctacttct ttcaccatcg caaacagcac atcttccact
    61201 ttgatctgcg tcgctttcca gtcgttatgc acgctctgtt ctgcgcctgg ttttgccatg
    61261 caatattgta gcaactccga aatggtcatt ttttactccc cttgaagtgt cgcgatgatg
    61321 cgacgcgatc cgccgtggat gcgatgttcc cccagccaga tgccttgcca ggtaccggtc
    61381 tgaatacgcc ctttatgcac cggcaataca agcgatgttc ccagcattga ggatttgata
    61441 tgagaaggca tatcgtctgc tccctcatag tcatgctcat aatttccgtt atcgggaacg
    61501 gcgcggagga aaaaacgctc catgtcgtgg cgtacggtgg gatcgcagtt ctcattaagt
    61561 gtcagagagg cggaggtatg ttgcagcaac agatgcagta agccgatgtt aacgcgcggc
    61621 atatcagcca gctgattcag aatttcatcc gttaccagat gaaacccacg agatttggcg
    61681 ctaagcgtga gcgtcttttg ataccacatg tgctgctcct tgataaaact ctcttaatca
    61741 gtttgcagca agacagcgaa aggataaagg tgtgattaaa aaaacagcat tcgggagagt
    61801 attacgctct cccgctctgt cagtattctg aattgacgat cacctcttca ccaaacgcac
    61861 ccgcttgtgg caagggtttg taggtagagt tggaggcgcg cagaatgcgg ataccacgag
    61921 cgccgacatc gcgtgcggcg gtaatatcgt tatcggaatc accataaaaa attcggatat
    61981 ttttatcctg cagccattgc gattttgtat tttgccctag tttatcgccc gcaaagatca
    62041 ccggattcat gttggtggca ggaatatgaa aattatccgc cagcgttttt gaaaccgttt
    62101 ctgttttggt cgggctacga ccagtcacaa agaagatcgc gtcaccgcgg cgtacatgca
    62161 tatcaatcag ctggcgagcg acctcttttg gaatgctgaa ttcatcccag ccattgttca
    62221 ttttttccca gaacacagga tttttcagat aatcttcgct ttctggcgag aaggtttttt
    62281 tgccgcgcca gaaacccgga ctggaaaaaa gtaccgtatc atcgatatca aaccccaccg
    62341 ccattggcgg acgtcctgcg aggctatttt caatttgtgc aaccgaaacc caatgaattg
    62401 gcgcctgttc agcgagtttg gctacgttag tacccggatt aagcggtgaa ggagatgagg
    62461 ccagggcaac agcggaactg tttagagcga acaataagca aacggcactc aatgcctgtg
    62521 tgatcttgcg catatttttc cctaaatagt cagtaagttg aaacttatta ttgtgcgttt
    62581 gttgaaaaaa ccatctgacc ataacgccaa ccgcggggga aaggaaggat tatctgcgga
    62641 tttgtcacat aaaaagagca tgatcacatt tggaatacct gtgcgataaa ccacacattg
    62701 ccacggttat tctgcttaca cattcacaca atggcactct tttggttctc gcaaacattg
    62761 ttcagggaat atactgatga taaatataaa tgcattcaat ggatgatgaa tgaaaatatc
    62821 gtggaattat atatttaaga acaaatggcg atttcacatt acaagcattt cactttttct
    62881 tatcatgctc gcggtttcaa tcgctttttt gcacttgcgt tttaatacct tgtccggtac
    62941 cgataaaatg cggcttgaaa tgtataagtc cacattatat tccaccatcg agcaatttta
    63001 tgttttaccc tatatgctct caacagacca tatcatccgt caggcggtaa ttacgcctga
    63061 cgatatgacg tccagcgaac tcaatcaacg aattgcacat ttcaatactc aactcaaaac
    63121 cgcagcaata tttattctgg atacccaagg taaggccatc gcttctagca actggcagga
    63181 ccccggcagc tatgtagggc aaaattatag ctatcgcccc tattataaac acgccatgtc
    63241 tggcttaaat ggacgctttt acggtattgg tagcactaca aatacaccgg gattcttcct
    63301 ctctacaagt ataaaagata aaggaaaaat tgtcggtgtt gtagtagtaa aaataagtct
    63361 taatgaaatt gaaaaagcat gggccgaagg tcctgaaaat attatcgtga atgatgaaca
    63421 tgggattata tttttaagtt caaaatcgcc atggcgaatg cgaacactgc aaccgttacc
    63481 tgttcaggca aaacaaaaac tacaatctac ccgtcaatat agtctcgaca atcttttacc
    63541 ggcagattat tatccctgtt ataccgtgag caattttact ttcctgaaag ataaaaaaga
    63601 acaactctgt ttattcccgc aatattatac acaacaaata gccattccag aatttaactg
    63661 gaaaatgaca attatggtcc ccttagataa cctgtactgg tcatgggcta tttcgttagt
    63721 cattacacta attatttacc tgctgttttt gttatttatt aaatactgga gaatgcgatc
    63781 tcatgcacaa caattattaa cactggcgaa tgaaacatta gaaaaacagg ttaaagagcg
    63841 tacatctgcc ctggaattga tcaatcaaaa attaatacag gagataaaag agcgcagtca
    63901 ggctgaacaa gtattacaaa ttacgcgtag tgaactggca gagtccagca aactggcggc
    63961 gcttggacag atggcaaccg aaattgccca tgaacaaaat caaccgttag ccgccattca
    64021 cgcacttact gataacgcgc gtactatgct aaaaaaagag atgtatccgc aggttgaaca
    64081 gaatctgaaa cacattattt cagtgattga gcggatgacg cagctcattt ccgaacttaa
    64141 agcatttgcc tcgcgccatc gcgtacctaa aggttctgcc gatgtcatca aagtgatgta
    64201 tagcgccgtg gcgttactta atcacagcat ggagaaaaat aacattgagc gacgaataaa
    64261 agccccatcc atgccgttat ttgtcaattg cgatgagctc ggtcttgaac agatattcag
    64321 taatttaatt agcaacgcct tagattctat ggaaggtagc tcttacaaac gactggatat
    64381 cgccattcgc caggcaaata acaaagttat tattaccatt aaagacagcg gtggcggttt
    64441 tgcacctgaa gttgtcgatc gcatatttga accatttttt accactaaac gtagaggaat
    64501 ggggttggga ctggcaatag tcagcgaaat tgtccgaaat tcgaacggcg cactccacgc
    64561 cagtaatcat cctgaaggcg gcgcagtaat gacattaacc tggcctgaat ggggagaaga
    64621 acatgaataa taaatccagt gaaattacta tagtttatat tgaagatagt gacgatgtac
    64681 gttttgcctg tgaacaaacg ctcacgctgg ctggttatcg tgttatttcc tgttgcgatg
    64741 cagaacacag tattcccctt attcaaagtc aggccaacat tattatactg accgatgtca
    64801 ggttacctgg aatatcagga ctagagctcc tcagttacat caacgaaatg gatagcaaaa
    64861 ttccggttat tttaattacc ggacacggtg acgtggaaat ggcggtggat gccatgcgta
    64921 atggtgcatt cgatttcatt gagaaaccaa gtagctcaga taaattattg agtattattg
    64981 ccagagcggt agaaaaacgg cgattggttt tagaaaatca gcaattactg gcgaatctgc
    65041 aacaggaaaa tggcccggtg ctgattggtc gtagtccgca gatgcaacaa ctgcgtaaaa
    65101 tgatccttaa tgtcgcagat accggtgccg atgtccttat ttatggtgaa acaggttgtg
    65161 gtaaagaagt cgtcgccaga atgctgcatc actggagtac ccgccgtcag gggcagtttg
    65221 ttgccttaaa ctgtgcaggc ttaccagaaa cgttgtttga aagcgaaata tttggtcatg
    65281 aggcgggcgc atttaccggt gcggttaaaa agcgtattgg aaaaattgaa catgcaaatg
    65341 gtggtacgtt atttctcgat gaaatagagg gaatgccatc aggaatgcag gtaaaactgc
    65401 tacgtgtatt gcaggaaaga accattgaac gattaggtgc caatcaatta attccggtga
    65461 attgccgtgt tattgcagcg accaaggagg atttactgcg tcgcagcgaa gaacatctgt
    65521 ttcgtctcga tctctattat cgtctgaatg tggttagcct gaatattcca cctctacgcc
    65581 agcgacgaga agatatccct gaactgtttt actggttcgc cagtcaggcg gcacaaaaat
    65641 ataatcgtcc tctgcctgat atttctccca tgttactggc ctggttgcag tcacaaagtt
    65701 ggcctggcaa tgtacgtgaa cttaaacaca atgcagagcg ttttgttctc ggtttactga
    65761 cccaccacca gccagtgcca atgactcagc aagaagagag tggtcttacc gcctgcattg
    65821 atgctttcga gaaaaagtta atcgaagata tgctgcgaca aacggaaggc caggtcagtt
    65881 tgacagcgcg gctattacag ctgccgcgca agactttata tgacaaactc aacaaacatc
    65941 agattcaacc gcaagtttat cgaccagaat catccagcta atgctgattc ctgaggatgt
    66001 aaccccaaat cgataccttt gcgggcgtaa tgttcaacta cccaggacca gaatgatacc
    66061 gggatcgtaa tcatcttcgc attgcgttcc cggttggcag cttctggcat accggggtaa
    66121 atcaccggat tgccgccagg ttgaggctgc gtagctaaca gataatccgc aaagaactgc
    66181 cctttctccc ctgccagatc ggtgaattga tcaggatcga taatcaacgc aaaaagatga
    66241 ttatgcgcag atccatccgt ctgatgatct tcggcgatcg tgtcaccacc agataagatc
    66301 cccgccatca tttcaatcat cagtgcaagg ccaaatcctt tatgctcacc aaatggcagt
    66361 aacgcccctg gaggattgct gaataacacc ccaggatcat tgcttggatt cccctgtgca
    66421 tcaatcaatt gtcccggcgc ggcctgcttc ccggtggacg acattacccg tgctttattt
    66481 agcgccatgc tactggtggc aaaatcaacc agaatcggcg gacgcccatc actgaacggc
    66541 caggcaaagc agaaagggtt agtggtcata cgcggttccg caccgcccca cggggcgacc
    66601 agtgcaggac gcccggtgac gttactcatg gccagcatcg ccaggccttg atcagcaacc
    66661 atttgcccgt aatgccccat acgccctaaa tgatgcgcat ggcacaacgt taacaggcaa
    66721 aaaccgcggt gttttgcttg ttcgatggca gcatttaccg ccacttttcc ggcgtgttga
    66781 ccaaagccat tgtgggcatg aaaacgttgt aaattacctt cacttgttaa gcattccggg
    66841 cgggcattcg cggttacatc gccacgagcg atattttcca gatatttcgg tagcagcgtg
    66901 acgccgtggc tggcaaagcc ataacgttcc gcttcaatca tatgttccgc cacatcctga
    66961 gcgatatcgg catcacagcc attaagttcc agcagcccgg cagctaacca acgcactttt
    67021 tcaacagcaa tttgtaatga catcgttggt ttcctcacat tagacctaaa attttccacc
    67081 aggtggcggc aaacagtagc aacaacaggt agccgatcac cgtcaggtac agtcccacac
    67141 gggtaaactg gcgtggcgtg aaggtatccg tacccataca caccatattt tgtggcgcgt
    67201 taatcggcag gatgaagcca aaactgacac tgaaggcgag cagaatggtc atgccgaccg
    67261 ggttaacgcc gagttctggc ggcagactgc ttaacaggct aatcaggatg ggcagcaatg
    67321 cggcagttag cgcagtggca ctggcaaacc ccaggtgaat aattatcaag aaagcggcga
    67381 gaatcgcgaa gatggctaat gatggcaagc catcgagacc aaaacctttc accacatagt
    67441 tcgccatcca tgaagctgcc tgcgtatcaa gcagcgtgga ccccaggctg ataccaatac
    67501 caaacataag caacgtgccc cactgcacac gtttttcgac ctctttccag ctcattacac
    67561 cgatgcctgg cagcaacata atcgccagtc cggcaagggt gacagaggtg gtatcaatac
    67621 tgtgtaattt tccgccagta gaccaaaaga gcagtaacaa tagcgaaata ccgatcaaac
    67681 gtttttcttt accagtggtt ggccctaact ctgcaagtgc ttttttaatg gcctcgctcc
    67741 ccccttcaac agcttccgtt tctggcggca acaatttacg tgcgaggaaa tagaggatcg
    67801 ccgacatggt gaggctccag ggcgcgcccg ctaacagcca gtcaagccag ctgacagaat
    67861 gcccggcgcc gaaagtttta ttgataaaac cgatagaaag caggttttgc gccgccgaag
    67921 tttgaatacc aacgttccag atactggtcg cctgggcgat aacaatcatc attgacgccg
    67981 ctaaacgtga atgtttatcc accttaaatg ccgcaatgac ccccatcata atcggcacca
    68041 cgcaagccgt acgggcagtg gcactgggta caaccaggct caggacgatg gtcaccacaa
    68101 tggcaccgat aataatgctg cggctgctgg cacctatttt cgacatagta aacagcgcaa
    68161 tgcgtttatc cagcccggta atggtcattg ccgccgcgat aaacattgct gctgccacca
    68221 gcgccagagc agaattagaa aaacccgaca gagtcatttt cagcgctttc gcggtgccaa
    68281 ggatcgtgtc cgggtgattc atatccgggg caaaaccaac cataaagata atcagtgcgg
    68341 aaataacaat tgcactggcg gtgtaatcca tcgcttcgct gatccagaca ataatggcaa
    68401 acaccagaat ggcgatcata ttttgtccag cgacgggcaa ggtatcgccc agtggcagaa
    68461 gggtaataat aattccggca atgatcgcca accacaggcc gggattaggg ataattcgac
    68521 gcttttgtgg tgcgccatgg gatattgatg acatattcac ctcattgtga aaaataaaaa
    68581 acctgcctgg ctattgcagg agctatgcca tctttaattt tttaataacg ctttgttatt
    68641 taatgatttt attatttatt ctttttattt tgtgcgcggt tttccgcaca gtaaataaaa
    68701 ggtgattctc ttaccacaca gataaaaaaa gggagcgata tgctcccttg atattcattt
    68761 atggattact taataacatt ccataacgtt tcgccaagca acgccgggtt tcttgtccat
    68821 gccatgcctg ctttatcgaa cgcacggaat ttatcctctg cactgccgct accgccagaa
    68881 ataatggctc cggcgtgccc catgcgtttt ccttttggtg cagtaacacc ggcaatataa
    68941 cctacgaccg gtttattcat ttcatcacga ataaattccg cggcccgttc ttccgcatta
    69001 ccaccaattt caccgatcat aattaccgcg tctgtttgcg gatcattttc gaataaacgc
    69061 agcgcatcaa taaagttagt ccccggaacc ggatcgccgc caataccaat acaggtggat
    69121 tgcccaagtc ccagcgccgt ggtttgcgct acagcttcgt aggttaatgt accggagcgg
    69181 gaaacaatcc caatacgccc tgggcggtgg atttcaccag gcataatccc gattttgcac
    69241 tcgcccggcg tgataactcc tgggcagttt ggcccaatca gccgcacatc cgggtggcag
    69301 tcaagcagcc gacgaacttt caccatatcc agtgtcggta caccttcagt gatcaccaca
    69361 accaatttga cgccagcttc aatagcctca acaacagaat ccagcacaaa cggtgcaggc
    69421 acataaatca cgctggcatc agcctcagtt tgtcgcatcg cttctttcat tgaatcaaaa
    69481 accgggcgat ccagatgccg ttgcccgccc ttacctggcg tcactccacc cacaatctgc
    69541 gtgccatacg caatagcctg ctctgtatga aatgtgccgt ttttgccggt aatgccctgc
    69601 accagtaccc gcgtgtgttt gttgatcaaa acgctcatct taacctcctg ttaattcagt
    69661 aacgcaatga tgcgtttagc ggcatcatcc agagaattga cggcttccac cgtcaggcca
    69721 ctttctgcca gtaaccgctg cccttcggca gcgttattac cggagagacg caccacgaca
    69781 ggcagggtga tacgggcctc attcagagca tgaataatgg ctctggcgat catgtcgcaa
    69841 cggacaatcc caccaaaaat attaaccaga atggctttca ctttgctgtc ggaaacaatc
    69901 agccgaaagg cttcactgac gcgctcctgg gttgcgccac cgccaacatc caggaagttt
    69961 gcgggttgtt caccgtatag cttgatgatg tccatggttg ccatagccag ccctgcacca
    70021 ttgaccatgc agccgatatt tccgtccagc gagacataat taagatcaag cttcgctgcc
    70081 tggctttcgc gtggatcttc ctgggtttca tcacgtagca cctggagttc cgggtgacga
    70141 tacagcgcgt tatcatccag cgatactttc gcgtctgcac acataaattc gcccgtttcc
    70201 cgcaacacca gagggttaat ttccagcagg gcgaaatcca gttcgttaaa ggctttccac
    70261 gcctgattaa ccaggcgact aaaggcagca aatagcccgt gttccagttg cagaacagca
    70321 aacatttcgc ggatatggca aggctgcacg cctgtcagtg gatcaatgct gacactgctg
    70381 attttctccg gtgtctcatg ggcgactttt tcaatttcca caccaccttc cgggctgaca
    70441 ataaacgtga cccgctggct ttcacgatcc accaccatgc caaaatagag ttcctggcgt
    70501 accggataga tgttttcgca cagcaaaata ctgctgacat actgcccttc tggcccggtc
    70561 tgataagtca ccagttgcga tcccaacatt tgctgtacaa aagcctgtgc ttccggcagc
    70621 tgcttaagca cttttacgcc gccagcttta ccgcgcccac cagcatgtac ctgcgcttta
    70681 agtaccgcgc ctttactcgg gcaagcaata tgttgccagg catctgccag ttgcgaaatc
    70741 tgttggatgg ctatctcttt tggacacggc atccccatgc cagccagtaa cgatttggcc
    70801 tgatactcat gtaaattcat atattccctc agtggtatgt gttatccgtg taatgcttta
    70861 ttattcaagg ccattgctgc ttcgtggatg acttcgctta aggtcggatg cgcatggatc
    70921 gcacaggcaa tatcctcgcc agaggcgcta aaagtcatcg ccagcgcaat ttcattgatc
    70981 agctccgatg cctgcgggcc aacaatcgct ccgccaagta cgcggtcagt gtgtttatcg
    71041 ctgtataacg tgcaacgccc accttcctgg cctaatgcta aggcgcgacc gttaccggca
    71101 aacagagaat ttcctttgtt aaagactctg ccagcggctt tcagtgaggc ctcgttttcc
    71161 cctacccacg caacttccgg ttgggtgtaa atcaccgaag ggatcagcgc aaaattaatc
    71221 ggctctaccg ccagtccggc aatttgatcc gcaaccacca caccttcagc catcgcttta
    71281 tgtgccagca tcggccctcg cacgacatca ccaattgccc acagcccggc tttcccggta
    71341 cggcataagt tatcgacggc aataccgcca cggttgtcgg cttcaagacc gagttgcacg
    71401 agatcgacac cagaaagtcg cggtacacgt ccaatggcaa gaattaattt atcgaagcgg
    71461 ctttcttctc gcttctcacc ctgccgccag cgcacatgca caccgtcatc ccgttgttca
    71521 atagcctcaa tctctactgc cagttgcatt ttcatcccac tggcaatcat tgctttacgg
    71581 acttcgttcg acaggcgagc ttccagtgca ggcaagaacg ttggcgccat ctccagcaac
    71641 gtgacgtctg aaccgacccg attccacaca gaccccagtt caaggccaat cacgccagcg
    71701 ccgatcacac cgagacgcgg cggcacagcg gttaacgcca gggcgccgcg attatcgaga
    71761 atttgctgat tatcaatggt aacgccaggc agttgacgcg gctgagaacc tgttgcaatc
    71821 accacattac gggcgcggat ttgttgatcg tttacacgta attgccaaat ctcatcccga
    71881 gcatgttcta acgttgcgag gccgcacagg tacttcactt tatttttctt aaacagcaag
    71941 ccgatgccca ttgtcaggcg gcttactatg gcgtctttac gttgaatcat cgcagctgca
    72001 ttgaaagata cgccgtcaac attaacgcca tgaatgctcg cctcatgctg cacttgcgcg
    72061 tacagttcgg aagattgcag caacgatttt gacggaatac agccgacatt gagacacgta
    72121 ccgcccggcg aaggttcgcc ctgtgcatta acaccatcat caatacatac tacggaaagc
    72181 ccattctgcg cagcccgcag tgccgctacg taaccacctg gcccaccccc cataactgcc
    72241 acatcaaaaa ttgtactcat cggcgccctc cttaaagatc cagcaacagt tgttccggtg
    72301 attccagtag ctcacgtatt gccaccaatg tctgcacggc ttcctgacca tcgataatgc
    72361 gatggtcata gctgagagca aggtacatca tgggacgaat aacaacctgg ccgttttctg
    72421 cgaccgggcg cggcgtgatg gcatgcatcc caagaatggc cgactgcggc gggttaatga
    72481 ttggcgttga catcatcgaa ccaaaagtac cgccgttggt gatagagaac gtgccgccct
    72541 gtaacgcctc cagcggcaat ttgccattgc gtgcctgtgt ggcgtattcg gcaatttgcc
    72601 gttcaatttc caccaaagag agtgattgcg cattgcgtaa cacaggcact accaggccgc
    72661 ggttgctgct cactgcaatg ccgatatcgc aataatcacg ccagataatc tcgttgccat
    72721 caacgctggc attcactaca gggaagcgct ccagcgcccg ggtaacggcc ttaacaaaga
    72781 aggacataaa gcccagcttc acgccgtgct tctcggcaaa acgatctttc cagcgggcgc
    72841 gtaaatccat cacgctctgc atgtttactt cgttgaatgt ggtaagaatg gcgttatttt
    72901 gttgggaagc cagcagcaat ccgctgacgt aagcgcgaca tcggctcacg acgttcctga
    72961 cgagcacccg gtgtcaaggg tttagctggc gcgatctccg ccacccgttc aggctgaatg
    73021 actggtgcag gagtaacgcg ctgtacatcc tctttcagga tacgtccatt tctcccgctc
    73081 cccgcaacgt cagcaagttc gacaccacta cgctgtgcct ccagccgtgc ggaaggcatc
    73141 gccagggttt cggtgactgg tgtgacggtt tcttctatga cagcttgcgg cttaagatgc
    73201 gccaacagtt gcgcggacgt gaccgtgcta ccttcgctga cgataatatt gcttaacacg
    73261 ccatcgtgcg gggccggtat ttccagaatg actttatcgg tttccagctc ggcaatcaca
    73321 tcatcgcgtt ttacgtgctc gccttcttgt ttacaccagg ttgtcagcgt gccttccgtg
    73381 acagattccg gtaatacagg gacagtaatt tcgatcataa aatgttctcc ttattatttt
    73441 tgtgcgcctt tcatggctga atatctgcaa acgcagcagc aagaaattcg tcgatttgtt
    73501 gtttatgtac acgcccatac cctgtcgccg gagctgccgc tgccgggcgg cctgcatact
    73561 gccagtaagg agtgttaatt ttcagtgccg ccagctcatg acgaatctgc cgccacgcgc
    73621 cctggttttc tggctcttcc tgcaaccaaa tccattcgca gcagttcggc caactcgcca
    73681 gaacatcatt cagttctgcg accgggaacg ggtagagttg ctccaggcga acaatcgcca
    73741 cttcgtcttc atgctcacgc tgcttacggg cttccagtac gtcgtaatag acctggccgc
    73801 tacacaaaat aacgcgctta actttctggg actgatgcaa ttgcggatcg gtgattaccg
    73861 gtttataagc gccatcagtg aattcacaaa gctcgctcat agcgccttta aaacgaagta
    73921 aacgcttact catcattatc actaacggtt tgcgcattgg gcgtaatgcc tgaccgcgca
    73981 acaaatggaa catttgtgct gactcactgg gcatcaccac ctgcatgttg ttttctgcgc
    74041 acagttgcag ccagcgctca ggacgcgcag aagagtgctc cggcccttgt ccgtcatagc
    74101 catgcggcag aagaatggtt aaaccgctat agcgatccca cttggtttcg ccagacgaga
    74161 taaactggtc aatagcaacc tgagcgccgt tggcaaagtc accaaactgt gcttcccaaa
    74221 taaccagttg ctgaggcgca gacgttgaat agccatattc aaaggctaag agcgcttctt
    74281 cattaagcac cgaatcataa acatcgaaag atgcctgacc agcgcggata tgctgtaacg
    74341 gcagataacg acgggcttca gtttgatgat gtaccactgc atggcgatga ctgaacgtgc
    74401 cacgccctga gtcttcacca gaaagacgca caccaacacc cgcatccaca agcgatgcat
    74461 aggccagcat ttcagccatt ccccagtcta ttggctgagt acctgcggcc atatcctggc
    74521 gtaacgccag ttgccgttta atggtaggat gtgcgaccac atcgggtggc agtgtagaaa
    74581 tgatttcacc atacgctgcc agcttttgcc gaggcaatgc cgtagatacc ggggcgctcc
    74641 agtgagggtt ggtcaaccca taccagttag cgctaaaaga gtgaattgca ggtttgagag
    74701 gctgcggttc acgtttctgg cagctatcca gccagtcgcg atagcgtgcc gtcatctcat
    74761 cttgttgcgc ctgagttaac aggccgcggc gggcaagaga ttcaccataa cgggcgagcg
    74821 taccagggtg agcatccact gcctgataca tttgtggctg ggtaagacgt ggctcatcgc
    74881 tttcgttgtg tccgtgctta cggaagcagc aaatatcgat gatgatgtcg cgccggaagg
    74941 tgtctcgcca ttcgcacgcc agttccatta cctggcaaac agcgtcaacg tcatcgccat
    75001 taacgtggat aattggggcc gctaccattt tggcaatatc tgtgcagtac cgtgaagaac
    75061 gcatatcctg caagcgggaa gtggtgaagc caatctgatt gttaatcacc agatgtagcg
    75121 taccgccagt gccgtagccc tgagtctgcg ataagttaaa cgtggtttgg ttgacgccga
    75181 ggccgcccaa cgcggaatcc ccatgaataa gtacgcccac cacttttgcc tggccatcct
    75241 caccacggcg ttcctgcctg gctcgcacct ggcccagcac caccggattc acgatctcca
    75301 gatgtgaggg gttatacgcc agcgccacat gcagcgaacc ggcaggtgtt tccagattgc
    75361 tggaataccc catgtggtat ttcacatcgc ctgaaccact gccgatcgtc tgcttgcctt
    75421 caaactcagc aaaaagctgt gccgggtctt tattcaacag attaaccaga acattaagtc
    75481 ttccccggtg cgccatgcca atgaccattt cctccacacc ttgcgcacgc aggcgttttg
    75541 tcagcgtatc gagcgccggg attgcacttt cgcccccttc cagagaaaac cgtttctgcc
    75601 caacataacg ggtatgcaga taacgctcta acgtctctgc cgccatcagt ttttcaaaac
    75661 gagcaataca ggtttgtaca tctgcttgtg gcgcattgct gctctcaatc cgtgataaca
    75721 accagttgat ttcttcacga ttttccagat gagcaagttc ataagcctgg ctacccgccc
    75781 aggcttgctc tagcaggtta agcagttgct taagtggcat ttgcgtggtg tgtgcgccaa
    75841 aggtgacgct aaactcctgc aacaagtctt cttcgcttaa tccccaaaaa ccaggcagga
    75901 gggagggaac gtcagccggg ggattcaaac caagtggatc aagctttgcg cgcagatgcc
    75961 cctgcgtgcg ccaggcattg atcagttgta ttatcgcagc ctgttttttt aaggtcgcac
    76021 cgctgacgtt gttatcaccg gatacgcttt ggacagtcag ttcaggtgaa agaaaaaaac
    76081 gccgccagtc ttccgggagt tgttccggcg tacgcaacca ggtctggtag taatcttcaa
    76141 gccaggtcgc gttatctcca ctaagcaacg tgccgggcga ggtcatgttc tccatgtttt
    76201 tctcctgatg gtcatagaca gccgcattac tgcacaatcc gtaccaacag ttaaaaacac
    76261 taattttcaa aaacttatag aaaatagatg atgtaaaagt gggcgaaaaa agggacagaa
    76321 tagtagggga tggctggagt caggaacagg cggagagatc cgcctgttga ttaaaatcac
    76381 atcaccgcag caaacgcctt tgccacgcga tgcacatttt gcgcgttaag cccggcgaca
    76441 cacatgcgac cgctggcgat gagatagaca ccaaattctt cacgtagtcg gtcaacctga
    76501 gcggttctta aaccggtata actgaacatg ccgcgctgat taagcagata atcgaaattg
    76561 cgctctggca tctctgtgct caataccttc accagttcct gacgcattgc cagaatgcga
    76621 gttcgcatct cttctacttc cgccagccag ctggctttca atgcctcgtc attcagcacc
    76681 gcagccacca ccttcgcacc aaaattcggc gggctggagt agttgcggcg aacggttgct
    76741 ttcaattgcc ccagtacgcg gcctgcggct tcggcatctt cacacagaac agaaagtccg
    76801 ccgacgcgct cgccgtaaag ggagaaaatt ttcgagaacg aattgctcac cagagcgggt
    76861 aatccagcgc tggcaatggc gcgaatggcg taggcatcct cttccatacc ggcaccaaat
    76921 ccttgatagg caatatcgag gaatggaata agctcgcggg ctttgagaat ttcaatcacg
    76981 gaatcccact ggtcattagt gagatcggca cccgttgggt tgtggcaaca tggatgcagc
    77041 aacacaatac tgcgggcagg taatgttttc agcgtcgcca acaggtcatt aaagcgcacg
    77101 ccgttagtcg cttcgttata ccaggggtaa gtgctcactt cgaatccagc cccggcgaat
    77161 attgctacgt ggttttccca ggtaggatcg ctgacccaga cgcctgattc cgggaagtag
    77221 cgtttcagga aatctgcacc cactttcaat gcccctgagc cgccaagggt ttgaatggtt
    77281 gctacgcgct gttgttgcag taccggatgg tccgcaccaa acagcagcgg cgcaatggca
    77341 tggcgatagc tgttaagccc ttccatcggt aaataaagcg aagcaccatg aggctgcgca
    77401 ttcaggcgcg cttccgcatc cgccacggct ttcagttgtg gaataattcc gtcttcgttg
    77461 tagtacagac cgatacttaa attcactttg tcgctgcgag ggtcttcttt aaaacgctcc
    77521 ataagcgtaa gaatcgggtc gccagcgtag gcgtcaactt tttgaaacac gcgatggttc
    77581 tccaggttta cgggcaggtg gttaaaacac aataaaccgg aagtaggcga agatcgagag
    77641 gatgttcagg ggcgaatggc aattagcaac agagtgagac tcatgacaaa cgtacatccg
    77701 ccagatgcac gacctttata agagcatgaa agaatatcaa ctcattgaat tggtaagatt
    77761 ttattggccg gataaggcat tcacgccgca tccggcacag acaatcaaat attacagaac
    77821 gattaatcca cgtatttcat cgcgactctt gaggtcaggc gcgtgataag ttcgtaagca
    77881 cttactttcg tcatttcagc gatacgttct acgggcaaac cttcgcccca taaaatgacc
    77941 gggtccccgg ctttatcctg cgcctgtgga cctaagtcta cgcagatcat atccatcgcc
    78001 acgcgcccga caatcggcac ttcgcgaccg ttcaccagca ctggcgtacc ggacggcgcg
    78061 gcgcgcggat aaccatcgcc atagcccatc gcgactacgc caagacgagt atcacgttcg
    78121 cttacccagg ttccaccata accgacaggc tctccggctt tatgctcacg cacggcaatc
    78181 aggctggagg ttagcgacat cactggctga cagccaaaat cggcaccggt ggagcgatct
    78241 tcaagcggag atacgccgta aagaatgatg cccgggcgca cccagtcaaa atgcgactgt
    78301 ggccagagta aaatgccacc cgacgcggca atggaacgtt gaccaggttt gccttcgcaa
    78361 aaggtattaa agatagcgag ttgcttctcg gttgcgccgc attttggttc atccgcgcgc
    78421 gcaaaatggc tgacgatatt caccggctga cgaacgtttt tgcactgggt cagacgatga
    78481 taaaacgcct cagcctgttc cggccttacg cccagacggt gcataccggt atcgagtttc
    78541 atccagacgg taaccggctc gtccaggcta gcctcttcca gcgcagccag ctgttcttcg
    78601 ttatgcacgg cggtatgaaa atgttgcgca gaaatcgtcg gtagatctct ggcatcaaaa
    78661 aagccttcga gtaaaagtac aggtttggtg attccccccg cacgcagtcg cagagcttct
    78721 tcgagacggg ctacgccaaa ggcgtcagca tcggggagcg ttcgcgcggt ctcaagaaga
    78781 ccgtgaccat aagcgttcgc tttcaccacc gcaaccattt tactggcagg cgccagttca
    78841 cgaagacgtt gcaggttgtg tcgcagagcg cggcggttaa tcacaacagt tgccgcttgc
    78901 atttgtgttc cttgataagt gtttgcttta attacctaat tcataaaata attattattc
    78961 gtcgtcgtac tgcggccccg catagttgtc gaagcgcgac cattgaccgt taaaggtcag
    79021 gcgtaccgtc ccgattgggc cgttacgttg tttaccgata ataatttccg cgatgccttt
    79081 taaatcactg ttttcgtgat acacctcatc acgatagata aacatgatca agtccgcatc
    79141 ctgctcgata gagccagatt cacgcaggtc ggagttgacc gggcgtttgt cggcacgttg
    79201 ttccagagaa cggttcaact gggacagcgc caccaccggc acgttcagtt ctttcgccag
    79261 tgctttcagc gagcgggaga tttctgcaat ttccagcgta cggttatcgg aaagcgccgg
    79321 tacgcgcatc agttgcaggt agtcgatcat gataagcccg atgccgccgt gttcacgggc
    79381 aatacggcgt gcgcgggaac gcacttccgt tggcgtcaag ccggaggaat catcgatata
    79441 gatattacgt ttttcgagca aaatacccat ggtgccggaa atgcgcgccc agtcttcatc
    79501 atcgagctgc ccggtacgga ttttagtctg gtcaacgcgc gacagcgacg ccagagaacg
    79561 catcatgatc tgttctgatg gcatctccag cgagaagata agcaccggtt tatcctgcaa
    79621 catcgccgcg ttttcgacga ggttcatcgc aaatgtcgtt ttacccatcg acggacgcgc
    79681 ggcaacgatg atcaaatccg acggctgcaa gccagcggtt tttttgttga gatcgtcata
    79741 accggtattt accccggtaa cgccatcgtg tggctgctga aacaactgct caatacgcgc
    79801 cacggttgcg tcgagcacat cggcgatgtt cttcggccct tcgtctttgt ttgcacgact
    79861 ttcggcaatt ttaaagacgc gggattcagc aaggtcgagc agatcttcac tggtacgccc
    79921 ttgtggatca aaaccagctt cggcaatctc gttagcaacg gagatcatct cacgaacaac
    79981 ggcacgttcg cgcacgatat ccgcatatgc actgatgttc gccgcacttg gcgtattttt
    80041 tgacagctct gccagataag caaaaccacc gacgctatcg agttgcccct ggcgttccag
    80101 cgattccgca agggtaatca gatcgatagg actaccgctt tcctgcaaac gcgccatttc
    80161 agtaaagata tgacggtgtg ggcgggtgta aaaatcgtct gccaccacac gctcggctac
    80221 atcatcccag cgttcgttat ccagcattaa accgcccaac accgactgct ccgcttcgat
    80281 cgagtgcgga ggcactttta gcccggcaac ttgtggatcg cgttcgcggg gttcagcctg
    80341 ctgtttgttg aagggtttat ttcctgccat agtgaatgga gttaccgaga taaagaatgg
    80401 gtcgaaactt taccatatga agcagaccct gacgatacgt tctggaggac acatggcaac
    80461 acgaattgaa tttcacaagc acggtggccc ggaagtactt caagccgtag agttcactcc
    80521 tgccgatccg gcggagaatg aaatccaggt cgaaaataaa gccatcggca tcaattttat
    80581 cgacacatat atccgcagcg gcctttaccc gccaccatcg ttacccagcg gattaggtac
    80641 tgaagcggca ggcatcgtga gtaaagtcgg cagtggtgta aagcatatta aggcaggcga
    80701 tcgcgtagtc tatgcacaat cggcattagg cgcttacagc tctgtgcata acattaatgc
    80761 agataaagcg gcgattctgc ctgcggcaat ttcttttgag caagctgcgg catccttcct
    80821 gaaaggctta acggtttatt atctgctgcg caaaacctat gaaattaaac ccgatgaaca
    80881 gttcctgttc cacgcagcgg ctggcggcgt tggcttgatt gcctgccagt gggcaaaagc
    80941 ccttggcgcg aaacttatcg gcaccgtagg aaccgcgcaa aaagcgcaga gtgcgctaaa
    81001 agcgggcgcg tggcaggtta ttaactatcg tgaagagaat ctggtcgagc ggttaaaaga
    81061 gatcaccggt ggtaagaaag tgcgcgtggt gtacgattca gtgggcagag acacctggga
    81121 acggtcgctg gattgcctgc aacgccgcgg cttaatggtg agttttggca actcatcagg
    81181 tgcggttacc ggtgtgaact taggcattct caatcagaaa ggctcgttgt atgtgacacg
    81241 cccttccctg caaggctata tcaccacgcg ggaggaatta accgaggcca gtaatgaact
    81301 gttctctttg attgccagcg gtgtgattaa ggtcgatgtc gccgagcagc agaaatatcc
    81361 gctgaaggat gcgcggcgtg cgcatgagat tctggaaagc cgggcgacgc agggttccag
    81421 cctgttgatt ccataaaaga aattgggctt ccacctggga agccctttct tttatagttc
    81481 ggctgtatgt agggtacagc tcgatgaatt tgtttgacgc gcaatagtga cagatttgat
    81541 tatcaattcc tattttgttc taaggataaa accttaggtt gtgatcatcc gcacaatccc
    81601 ttagtaacgc cagcggtcat aacgctgata tttcggcact tttggtgctt taatcgccct
    81661 gatcacccac accaccgcaa ccgccaacag cagccacggc agcaacttaa tcatcaatgc
    81721 cagcataccg ccgaggaaca taaaggccgt cgccacaatc agcgcggcga taatacccag
    81781 caacgaaaca ccggtgacca tcagcatgac aaaaaagcca attacaaaaa gtagttccag
    81841 catgatgctc tcccaaatat gaaatctctt gctggcatta caagaatcat gccaaaaata
    81901 atctattgat ttaacagcaa aacgccccgc aacggtgcgc agggcgtggt gaatttgact
    81961 actttttggt gaaaagttaa cgcttatccg ccaccagttt gagcgcgtgt tccagcacat
    82021 taatgtctgc acccgcttta tgggcatttt cacttaaata acgccgccac tgccgcgcgc
    82081 caggaatacc ctggaacaag cccagcatat gccgggtaat atggccgaga tacgtaccct
    82141 ggctgagttc acgctcaatg tacggataca tggcgcgcac taccgccacc ggatcggcat
    82201 cggtatccga ggaaccaaaa atctctcggt ctaccgccgc cagaatcccc ggattctgat
    82261 acgcctcgcg cccgaccatc acgccatcca tatgttgcaa atgcgcttta gcttcttcca
    82321 gcgacttgat accaccgtta atcgacatcg tcagatgcgg gaagtcacgc ttcagttgat
    82381 acacacgcgg ataatcgagc ggcgggatct cacggttttc tttcggactt aacccagaaa
    82441 gccaggcttt acgtgcgtgg atgatgaaca tctcacactc acctttgccg gaaacggtgt
    82501 tgatgaaatc gcagagaaat tcatagctgt cctgatcatc gatgccaata cgcgtcttca
    82561 ccgtcaccgg aatcgacacc acatcgcgca tcgctttcac gcagtcggca accagctgcg
    82621 cattacccat cagacacgca ccaaacatgc cgttctgcac ccggtcagac gggcagccga
    82681 cattcaggtt gatctcatcg tatccacgcg cttctgccag cttcgcacac tgtgccagcg
    82741 ccgctggatc gctacccccg agttgcaacg ctaccggatg ttcttcttca ctgtacgcca
    82801 ggtaatcacc tttaccgtga ataatcgccc ctgtggtcac catttcggta tacagcaacg
    82861 tattgcggga aagcagacgc aggaaataac ggcaatgtct gtccgtccag tcaagcatcg
    82921 gggcaatcga gaaacggtaa gcgggataaa cagtgtgtga ttctgctggc atggcgactt
    82981 tttaagcatc tggtaaattg ggggcgctac tatagcataa cgaatatgcg atcaggcaat
    83041 gtgacagcca tgacaccctg ttctcggcgt ttaagtgaga actatggtaa agtaaggaca
    83101 ttcttaaccc cactgtcgag gtgcccaatg gaaaagacca caacgcagga gttattagcg
    83161 caggctgaaa aaatctgcgc acagcgtaat gtgcgcctga ccccacagcg tctcgaagtg
    83221 ttgcgcctga tgagtctgca ggatggcgct atcagcgctt atgatctgct tgatttgctg
    83281 cgcgaagctg aaccgcaagc caagccgcca acggtttatc gcgcgctgga gtttctgctt
    83341 gaacaaggtt ttgtgcataa ggtggaatcc accaacagtt atgtgctctg tcatctgttc
    83401 gatcagccca cccatacttc agccatgttt atttgcgatc gctgcggcgc ggtgaaagaa
    83461 gaatgtgctg aaggcgtgga agacatcatg catacgctgg cggcaaaaat gggttttgcc
    83521 ctgcggcata atgttattga agcacatgga ttatgttcag catgtgtaga agtagaagcg
    83581 tgtcgtcatc ctgaacagtg ccatcatgac cactctattc aggtgaaaaa gaaacctcgc
    83641 taaaagggtg tacatccttg tacatgtcgg gcaggaggga ttaattacca gcggtaatca
    83701 tggcgttttt cccaactatc aacctctttt tctgcctgat ctttctgata accgtagcgt
    83761 tcctggattt tacccactaa ctgatcacgt tttccttcaa tgatcgtcat atcatcatcg
    83821 gtcagtttgc cccattgctc tttcacttta cctttaaact gtttccagtt accgccggct
    83881 tcatctttat tcataatcaa gacctcatcg ttaggttgtg aatgagagta cgttcacttt
    83941 tcttctgaac gtgagattaa gtgtagtcag caatttggct taggattatt tattcagaat
    84001 ttttaaccgt cacgttgcga caaaccaggt atcgttacgc cagtgacgcc gccagatagc
    84061 cgccagagaa agcccgcgta acgccagaaa gacggttaac gccagccaca aaccatgatt
    84121 ccccagccac ggcagcgtaa ggagcgtcag tgcaaaacct gcggcggcca ccgccatact
    84181 gttacgcatt tcggcggcac gcgttgcgcc tataaacatg ccgtccagca gataacacca
    84241 gacgccgacc aacggcaaaa tcacctgcca gataagatag cggtcagcca gctgctgaat
    84301 ctgggttaac gacgtcagca acgcaatgat gtgttcccct gccagcaaat aaaccaccga
    84361 aaacagtaac gctacgatcc ccgactggcg gcacgctgcc cgccagacat ccagcaattg
    84421 actaccgtcg cgcgcaccgt atgcctgacc ggaatgtgct tcaaccgcgt aggcaaaacc
    84481 atccagcgca taggcggtaa aggtgagtag cgtcatcaga accgcgttaa cagcgataat
    84541 gtcactcccc agtcgcgcgc caagtacagt gatcgcgccg aaacagagtt gcagcaacag
    84601 cgagcgcagc atgatatcgc ggttaagcgc cagcaagtga cggaagttac ctcgccaggc
    84661 tgttttcagc atttcgccgg agatcccgcg tagtttgagg attttacgca ccattagcag
    84721 accaatcagc aatgttgcat attccgcaat aaccgttgcc agagccgcgc cctgcacgtt
    84781 catatgcagc cccatcacca gccagacatc cagcacaatg ttgaggatat tgccgaccac
    84841 taacaaaatt actggcgcac gggcatattg cacgccgagt aaccaaccaa gtaataccag
    84901 attcgccagc gacgccggtg cgcttaacca gcggatttca agaaagcgtc gcgcctgttc
    84961 caggactgct tcactaccgc caacaatatg cagcgccaga tcgataatcg gcgtacgcag
    85021 cagcgcaatt aacgccccag cccccaacgc caacagcaac ggttgcacca gcgcacgggc
    85081 taatgcctga ggatttttag caccataagc ctgtgcagtc agcccggtgg tgctcatgcg
    85141 taaaaacagc aacaacataa agagaaagct ggtcgccgtt gcaccaaccg ccacgccgcc
    85201 caaataaacc ggactatcaa gatgaccaat taccgccgta tcgaccagtc ccagcaacgg
    85261 aacggtgata ttggagaaaa tcatgggtaa ggcgagatgc cagagtgctt tatcagatga
    85321 agtgaggaat gccatgcaga caagcctgat gaagagagat gaaaaacaaa ccgcgacgcc
    85381 aggcggcatc gcggtctcag agatatgtta cagccagtcg ccgttgcgaa taaccccaac
    85441 cgccagcccc tcaatggtga agctctgctg acgaaggtca acgacaattg gtttaaactc
    85501 gctgttttct ggcaacagtt cgactttatt gccctgtttt ttcaggcgtt taacggtaac
    85561 ttcgtcatca atacgtgcga caacgacctg accgttacgt acatcctgag ttttatgcac
    85621 tgccagcaaa tcgccatcca taatgccgat atctttcatc gacatcccgc tgacacgcag
    85681 caggaaatca gcattcggct tgaacaagga agggtcgacc tgataatgac cttcaatatg
    85741 ctgttgcgcc agaagcggtt caccggcagc cacacgacct accagcggca acccttcttc
    85801 ctcttcctgc aataaacgaa tcccgcgtga tgcgccggaa acaatttcaa taacgccttt
    85861 gcgtgccagc gccttcagat gttcttcagc cgcgtttggg gaacggaacc ccaaacgctg
    85921 cgcgatttcc gcacgcgtcg gcggcatacc tgtctggctg atgtgatcac ggatgagatc
    85981 aaacacctct tgttgcctgg ccgttaacgc tttcattccg ccccctgggt gtatatacag
    86041 ttatgctgtg agtatataca gcaaaaggcg attttggaac cataaactgc acaataaacc
    86101 agagatttat cgaaatctgg aagggttatc caaaatgcga ccataacagg atgcaccagg
    86161 taatcacggc gacgataatg gcaatcagca ctgcggcgga tcccatatct ttagcgcgtc
    86221 cggaaagctc atggtattca gagccaattc ggtcaaccac cgcttcgatg gcgctattga
    86281 gaatttccac aatcatcacc agcatcacgg agctgataag cagtacgcgg gtaatcgcgt
    86341 ccacatccag ccagcaggcg ataaccaccg ctaacaatac cgctacacct tcctgacgga
    86401 atgccgcttc gttgatccat gcagcgcgta aacctttcca ggaatagcca gcagctttga
    86461 taattcgggt gaatccagtg gtattattgg ccattgaaag aaccttttta cattatgagc
    86521 gtcaatatca gtgtaccgtt aaatccgatg attcgggttt gcgtaacgcc gcgcgaaaca
    86581 tgagcggata ccacagaatt tcccatgact ttctgctatc cttgccgcgc atttgcatta
    86641 ttaaccagag gctttacatc gtttatgtcc ggctggccac gaatttacta caaattactg
    86701 aatttaccat taagcatcct ggtaaaaagc aagtctattc cggcggatcc tgccccggaa
    86761 ctggggctgg atacctctcg tccaattatg tacgttttgc cgtacaactc gaaagctgac
    86821 ttgctgacgt tgcgcgccca gtgtctggca catgacctgc ctgacccgtt agagccgctg
    86881 gaaatcgacg gcacgctact gccgcgctat gtgttcattc acggcgggcc gcgtgtgttc
    86941 acctattaca cgccgaaaga agagtctatt aagctgttcc acgactatct cgatttgcac
    87001 cgcagcaacc caaatctgga tgtgcagatg gtgccagtat cggtgatgtt tggtcgcgcg
    87061 ccggggcgtg aaaaaggcga agtgaacccg ccgctgcgta tgcttaacgg cgtacagaaa
    87121 tttttcgctg tactgtggct cggtcgcgac agttttgtgc gtttctcgcc gtcagtttcg
    87181 ctgcgccgta tggcggatga acacggcacg gataaaacta tcgctcagaa actggcgcgc
    87241 gtagcacgta tgcactttgc ccgtcaacgc ctggctgccg tgggtccacg tctgcctgct
    87301 cgtcaggatc tgtttaataa gctgctcgcc tcccgcgcca ttgcgaaagc ggtagaagat
    87361 gaagcgcgca gcaaaaaaat ctcccatgaa aaagcgcagc agaacgcgat tgcgctgatg
    87421 gaagagattg cggcgaattt ctcttacgag atgatccgcc tgactgaccg cattctgggc
    87481 ttcacctgga accgacttta ccagggtatc aacgtccata acgccgagcg cgttcgccag
    87541 ctggcccacg acggccatga gctagtatat gtgccttgcc accgcagtca catggactac
    87601 ctgctgcttt cttacgtgct gtatcaccag gggctggtgc cgccgcatat cgccgccggg
    87661 atcaacctga acttctggcc ggccgggccg attttccgcc gtctgggggc gttctttatt
    87721 cgccgtacgt ttaaaggcaa taaactttat tccaccgttt tccgtgagta tctcggcgaa
    87781 ctgttcagcc gtggttattc cgtcgagtat ttcgtggaag gcggtcgttc ccgtacgggg
    87841 cgtttgctgg atccgaaaac cggtacgctg tcgatgacca ttcaggcgat gctgcgtggc
    87901 ggcactcgtc cgattacgct gattccgatc tatatcggtt atgagcacgt catggaagtg
    87961 ggtacttacg ccaaagaact gcgcggtgcg acgaaagaga aagagagcct gccgcagatg
    88021 ctgcgtggtt taagcaagct gcgtaatctc ggtcagggtt acgtcaactt cggtgaacca
    88081 atgccgttga tgacctacct taaccagcac gtaccagact ggcgtgaatc tatcgatccc
    88141 atcgaagcgg tgcgtccggc ctggttaacg ccgacggtca ataatattgc tgccgatctg
    88201 atggtacgca ttaacaacgc aggcgcggca aacgccatga acctgtgctg tactgcgctt
    88261 ctggcgtcac gtcagcgctc actcacccgc gagcagttaa ccgagcaact caactgctac
    88321 ctggatctga tgcgcaacgt accttactcc acggactcta ccgttccttc agccagcgcc
    88381 agcgagctta tcgatcacgc gctgcaaatg aacaagtttg aagttgagaa agacactatc
    88441 ggcgacatca tcattctgcc gcgcgagcaa gcggtgctga tgacctacta tcgcaacaac
    88501 attgcacata tgttggtgct gccttcgctg atggcggcaa ttgtcaccca gcatcgccac
    88561 atctcccgcg acgtattgat ggagcacgtc aatgtgcttt acccaatgct gaaagcggag
    88621 ctgttcctgc gctgggatcg cgacgagtta ccggacgtta ttgatgcgct ggcaaatgag
    88681 atgcaacgtc aggggctgat taccctgcaa gatgatgagt tgcatatcaa cccggcgcat
    88741 tctcgcacgc tacagctgct ggccgcaggc gcgcgcgaaa cgctgcaacg ttatgccatc
    88801 accttctggc tgttgagtgc caacccgtcg atcaaccgcg gtacgctgga gaaagagagc
    88861 cgcaccgtcg cgcaacgtct ctccgtgctg cacggtatca acgcgccgga gttcttcgac
    88921 aaggcggtgt tcagttctct ggtgctgaca ctgcgtgatg aagggtatat cagcgatagc
    88981 ggcgacgccg aaccggcaga aacgatgaag gtgtatcagt tgctggcgga gttgattaca
    89041 tcagacgtgc gtttgacgat tgagagtgcg acgcagggcg aagggtaatc agtgagaatt
    89101 gccggatgcg acgcaaacgc cttattcggc ctaccatgat ctgcaaattc aataaattgc
    89161 gatatgttgt gtaggcctga gaagcgcagc gcatcaggca acccagaaga aagccggatg
    89221 atcatccggc ttttttacat catcagaaat gccagtaact cattgccagc cctaaaaaca
    89281 gtaccagacc aacatagtta ttattcataa atgctttaaa gcaggcttca cgctcgcggt
    89341 tggcaatcag tttttgttga taaacaaaca gcgcgccagc taccagaatt gaccagtaat
    89401 atccccagcc taagccattt aactcaccga tgatcgccat cagcgctagt acgccaatct
    89461 gcaaaatacc aataatcaat ttatcgtatt ggccgaacag gattgcggtg gatttaatgc
    89521 caatcttcac atcatcatcg cggtcaacca tcgcatactg cgtgtcgtaa gccaccgccc
    89581 agagaatatt ggcgaggaac attaaccagc aactcaatgg caccgactca ctcacagcgg
    89641 caaaagccat tggaatcgac cagccaaacg ccgcgcccag caccacttgc ggtagatggg
    89701 tataccgctt cataaacggg tatacccacg ccagcgccag cgcggcaatc gacaacagaa
    89761 tagtcatcgt attcagcgtc agcaccagta aaaatgaaat cagtaccagc actacaaaca
    89821 gcgcgcgcgc ctctttctcc gttaccgcgc cgctgggaag tggtcggttc gccgtgcgct
    89881 taacatgacc atcaaacttg cggtcagcat aatcattcac cacgcaacca gcagcgcgca
    89941 tcagccagac gcccgcgaca aacaccgcca ggatccagag ctggggaacg cccggtgtcg
    90001 ccacccacaa cgcccataat gttggccaga gcagcagtaa cgcgccaatt ggcttatccg
    90061 tacgcattaa gcgatgaaac gccagcagct tattctgcgt cagactccac tccatatttt
    90121 tttcctctta gtacaacggt gacgccggta aaaacagttc tgttagcaac agcggtttac
    90181 cgcttaatcg caggcgggaa cgtcgccccc acagtccggc atcacggcct atctcaataa
    90241 agtcccgggt taatgtcgat gatgtgaaca gatagcgtcc taacggcgtt ttacctaatt
    90301 tttgtaacgc cagctccggc ccgcttaacg ttgacacagg aacgacggta cgaccggcaa
    90361 gccacggttc accatcggca cataacaaaa tttcacgtaa ccagtaacga gactctttcg
    90421 gcagcagtgg cagttcttcg gggatttcat tctgctcgac aaacccttcg cggatcatcg
    90481 tcacgctcac cgtttttccc tgctgttcaa aacgttttgt catggaatct tccagcaaca
    90541 gccagtcgag cagttgcggc tccagggcag ggatctctgt aaaatagcgc agcgcacgca
    90601 gttgcgttaa cgcggggtgt gacatgccga actctccgtt acataacgta aaggcattgt
    90661 atcgcagaaa aatatttacg ggagatgtaa agcaaattaa gtgaactaat gcgcaacaaa
    90721 aacgcaacaa tagtacagga ggagaaaaaa aggtgcgcca ggagacgcac cagttgttgc
    90781 aaaatcagca ctgtggggcg taattacccc ttgcctttta cactgctgat aaaggtggaa
    90841 cgggcagatg tcgaccccag gcgttcagct tcatcaagca gttttaacgc cttatcaaca
    90901 tcacctttcg cgacagcgtt tttgatcgcg gtattaaaat aactttccgt gtcgttgagc
    90961 atcggctcgc ttttcttcac cggtgccgga gcgggtgcag ccacagctgg tgccgccgtg
    91021 ttgcctaccg taaccggagc tggagcggaa gaaccaaata gtggtcctac caacacgctg
    91081 gagctggagt tcgttttcac tttcagtttc agtaagccat cggtggtatg acgagcaacc
    91141 ggatcgggaa tatccgggat cgagttaccg acgcctttgg cataggcttt agccgggtcg
    91201 agcagttgag tcgtctgctg gagatctttt tccgtggtaa agaccagaac ataaagtttt
    91261 tgctgcccca acgccggtgt caggcgcata acgccttcca gccgatctgc actcatcacg
    91321 ccgggttcct ggtaggtgaa ataactgctg gggaagaagg ctgatggggt catgttctga
    91381 tcaagaatca gcacattcgg cgcaaatacg ctggtttgtt tgttcacttc gctggtcagc
    91441 gtcagtgtca gttcaccaat gtttgccggg acgctgtacg cagcaaccgg accactgatg
    91501 ccagggacat tcagttgttg gccgccggtc gacagttgag tagtctgggt tttagattga
    91561 tcgaccggtg tccaggtgag ttgttgcagc gcggcagatg gaatggctgg cgcgtcgctg
    91621 gtgttttgcg gtacgtagtt aacatcggca agactgattc caggtgcgct tgccagcaac
    91681 cctgctgata aacagagagc gatgagactt ttattcattt tcattgtttt cacctcaaaa
    91741 tctggagctc agcggtagcc aggcaatagc gcgctaaacc cgataatcag aggggctttc
    91801 gccccttaaa gctgttttta tgccggatgc ggcgtaaacg ccttatccgg cctactttcg
    91861 gcagcagttg taggcctgat aagacgcaac agcgtcgcat caggcgttgg ttgccggatg
    91921 cggcgtaaac gccttatccg gcctactttc ggcagcagtt gtaggcctga taagacgcaa
    91981 cagcgtcgca tcaggcattg gttgccggat gcggcgtaaa cgccttatcc ggcctacttt
    92041 cggcagcggt tgtaggcctg ataagacgca acagcgtcgc atcaggcatt ggttgccgga
    92101 tgcggcgcaa gcaccttatc aggcccaggt tttgctatta ccaccagatt tccatctggg
    92161 caccgaaggt ccactcgtcg ctgtcgccac gaccgaagct accgccgttg aaatcagccg
    92221 acacagcctt gccgtagtat gggttggttg aagagctacc ggtgtagtca taaccccatt
    92281 tctcatccca cttggcgtaa gttgcgaaga cacgaatagc cgggcgtgac cagatgctgt
    92341 cgccagcctg ccattgttgt gcgagggtaa ttttgtactg attgttcttg tcgccggtgc
    92401 gctgggattc gacgttgtcg tagccgattt ccatcacggt gctcatgatt ggcgtccact
    92461 tgtacatcgg gcgaataccg acggtccacc acttggtgcc gttgtcgtta tcccagttga
    92521 tatcctggta catacccacg tacatcatgt cccagttgtc gcccatggag atcgcaccgt
    92581 ggtcgaggat acgcagcatg tgaccgttgt tattgatatt gtaggcaaat ttttcgttat
    92641 caaacgcgac gccagaaccc tgcgacagac ctttaccctg cgaggtcatc gagtcagtag
    92701 cgtactgaac aacaaacttg ttaaaaccct tcaggacact ctgagtatgt tcagcagtga
    92761 ataaccagcc gtctttcgat gcgccatcaa ccagacgata gttatcacgc aggttggcac
    92821 gaccgtagtc gacacccagt tctaatgtgc cgcccgggtt gatttccatc tgcgctaaac
    92881 gcacatcgaa aacgtcgttc gcggtttcgt tggtatagtc ataaatattg ttgctggcga
    92941 aagaggaaga accacccgct tcagaggagc gggttgctgc cagagagagt ttaccgaagc
    93001 caacatcgat gttttccaga ccggcaccag gaccagaaat atcccagtag tagaagtcga
    93061 tcatatgaac gtcatgacgt tggtagaagc gcttacctgc ccagatggtg gagcctggca
    93121 gccattcgat caggttttta ccctgcacgt ttgcttcacg gaaggccgga tcggtagctt
    93181 cccagtcatt ctgttgcgcg acggaatagg ccacgttagt gtcgaaatag aagctcttat
    93241 cgccctcttt ccacacttcc tgacccaatt ttaattcagc ataagtttca cattcgttgc
    93301 caagacggta tttactttga gcaccggtag tctggaaaca ctgttgttcg ccgccgctac
    93361 ctgtccagcc aataccggaa cgtgcatagc cgtggaaatc aacagccatt gcctgagcag
    93421 acattacgcc cgctgcgacg gcaaccgcca gaggaagttt gcgcagagta atcatcattc
    93481 tatctcctga gtcattgctt ttcttttttc acatcacctg tgacaggctt tgtgtgtttt
    93541 gtggggtgct taaacgcccg gctccttatg cagtcgacga catgcagtgc catcctcacg
    93601 gaacagatgg caacgctctg gcggcaggcc gatagcgaat gtggcacctt cttctaccaa
    93661 caccacgtcg ttctggcggt acaccaggtt ttgacgaatg gaagggatct ggatatggat
    93721 ttgcgtttcg ttgccgagtt gctcgacgac ctgaacttca ccctcaagga tgacgtcagc
    93781 gatatcactc ggcagtagat gttccgggcg aatacccagt gacatattgg ctccaacctg
    93841 gacatcacgg ctttcgaccg gtagccagac ttgctgacga ttcggcatcg gcagctccac
    93901 ctgcacttga tcgattgcgg tggcggtcac ttttactggc aggaagttca tctttggaga
    93961 accgataaat ccggcgacaa aacggtctgc cggatagtga tacagctcca gcggtttccc
    94021 aacctgcgcc acgcgaccag cgtccagcac cacgattttg tcggccagcg tcatcgcttc
    94081 gacctgatcg tgggtgacgt aaatcattgt gcggcccagg cgtttatgca gacgggagat
    94141 ttcgatacgc atttgcacac gcagtgcagc atcgaggttg gagagcggtt catcgagcaa
    94201 aaatacgctt ggctcggcca ccagcgtacg gccaatcgcc acacgctgac gctgaccacc
    94261 ggagagcgct ttcggtttgc gatccagcaa atgcgccagt tgcagcactt ccgcaacctg
    94321 gttaactcgc tggttaatca cttctttttt cgcgccagcc agtttcaggc caaatgacat
    94381 gttttctgct actgacaggt ggggatagag cgcgtaagac tgaaacacca taccaacgcc
    94441 gcgttctgca ggcggagtgt cattcatccg tttctcaccg atgaacaggt cgccgctggt
    94501 gatcatctca agcccggcaa tcatgcgcag taaagtcgat ttaccgcagc cagacggtcc
    94561 gacaaacacc acgaattcac cttcatggat atcgagattg atatctttcg ataccacgac
    94621 ctcgccccag gctttcgtta cattttgcag ctgtacgctc gccatgccct tctccctttg
    94681 taacaacctg tcatcgacag caacattcat gatgggctga ctatgcgtca tcaggagatg
    94741 gcttaaatcc tccaccccct ggctttttta tgggggagga ggcgggagga tgagaacgcg
    94801 gcttctgtga actaaaccga ggtcatgtaa ggaatttcgt gatgttgctt gcaaaaatcg
    94861 tggcgatttt atgtgcgcat ctccacatta ccgccaattc tgtaacagag atcacacaaa
    94921 gcaacgatgg ggcgtagggg ccaggaggat ggaaagaggt tgccgtataa agaaactaga
    94981 gtccgtttag gtgttttcac gagcacttca ccaacaagga ccatagatta tgaaaataaa
    95041 aacaggtgca cgcatcctcg cattatccgc attaacgacg atgatgtttt ccgcctcggc
    95101 tctcgccaaa atcgaagaag gtaaactggt aatctggatt aacggcgata aaggctataa
    95161 cggtctcgct gaagtcggta agaaattcga gaaagatacc ggaattaaag tcaccgttga
    95221 gcatccggat aaactggaag agaaattccc tcaggttgcg gcaactggcg atggccctga
    95281 cattatcttc tgggcgcacg accgctttgg tggctacgct caatctggcc tgttggctga
    95341 aatcaccccg gacaaagcgt tccaggacaa gctgtatccg tttacctggg atgccgtacg
    95401 ttacaacggc aagctgattg cttacccgat cgctgtcgaa gcgttatcac tgatttataa
    95461 caaagatctg ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc tggataaaga
    95521 actgaaagcg aaaggtaaga gcgcgctgat gttcaacctg caagaaccat acttcacctg
    95581 gccgctaatt gcggctgacg ggggttatgc gttcaagtat gaaaatggca agtacgacat
    95641 taaagacgta ggcgtggata acgctggcgc gaaagcgggt ctaaccttcc tggttgatct
    95701 gattaaaaac aaacacatga atgcagacac cgattactcc atcgcagaag ctgcctttaa
    95761 taaaggcgaa acagcgatga ctatcaacgg cccgtgggca tggtccaaca tcgacaccag
    95821 taaagtgaat tatggtgtaa cggtactgcc gaccttcaaa ggtcaaccat ctaaaccgtt
    95881 cgttggcgtg ctgagcgcag gtattaacgc cgccagtccg aacaaagagc tggcgaaaga
    95941 gttcctcgaa aactatctgc tgactgacga aggtctggaa gcggtcaata aagacaaacc
    96001 gctgggtgca gtagcgctga agtcctatca ggatgaactt gcgaaagatc cacgtattgc
    96061 cgccaccatg gataacgctc agaaaggtga aattatgccg aacatcccgc agatgtccgc
    96121 tttctggtat gccgtgcgta ctgcggtgat caacgccgcc agcggtcgtc aaactgtgga
    96181 tgctgcgctg aaagatgcac agactcgtat caccaagtaa tgctgtgaaa tgccggatgc
    96241 ggcgtaaacg ccttatccgg cctacgaaat tgaaacgtat gtaggcctga taagacgcgt
    96301 cagcgtcgca tcaggcagtt gttgtcggat aaggtatgaa agccttatcc gtcctggaat
    96361 gaggaagaac cccatggatg tcattaaaaa gaaacattgg tggcaaagcg acgcgctgaa
    96421 atggtcagtg ttaggtctgc tcggcctgct ggtgggttac cttgttgttt taatgtacgc
    96481 acaaggggaa tacctgttcg ccattaccac gctgatattg agctcagcgg ggctgtatat
    96541 tttcgccaat cgtaaagcct acgcctggcg ctatgtttac ccgggaatgg ctggaatggg
    96601 attattcgtc ctcttccctc tggtctgcac catcgccatt gccttcacca actacagcag
    96661 cactaaccag ctgacttttg aacgtgcgca ggaagtgttg ctagatcgct cctggcaagc
    96721 aggcaaaatc tataattttg gtctttatcc ggcgggcgat gagtggcaac tggcgctcag
    96781 cgacggcgaa accggcaaaa attacctctc cgacgctttt aaatttggcg gcgagcaaaa
    96841 actgcaactg aaagaaagcg ccacgcagcc cgaaggcgaa cgcgcgaatc tgcgcgtaat
    96901 tacccagaat cgtcaggcac tgagtgacat taccgccatt ctgccggatg gcaacaaagt
    96961 gatgatgagc tccctgcgcc agttttctgg cacgcagccg ctctacacac tcgacggtga
    97021 cggcacgttg acgaataacc agagcggcgt gaaatatcgg ccgaataacc aaattggttt
    97081 ttaccaatcc attaccgccg acggtaactg gggggatgaa aagctaagcc ccggttacac
    97141 cgtgaccacc ggctggaaaa actttacccg cgtctttacc gacgaaggca ttcagaaacc
    97201 gttcctcgcc attttcgtct ggaccgtggt gttctcgctg atcactgtct ttttaacggt
    97261 ggcggtcggc atggttctgg cgtgtctggt gcagtgggaa gcgttgcgcg gcaaagcggt
    97321 ctatcgcgtc ctgctgattc tgccctacgc ggtgccatcg ttcatttcaa tcttgatttt
    97381 caaagggttg tttaaccaga gtttcggtga aatcaacatg atgttgagcg cgctgtttgg
    97441 cgtgaagccc gcctggttca gcgatccgac caccgcccgc acgatgctga ttatcgtcaa
    97501 cacctggctg ggttatccgt acatgatgat cctctgcatg ggcttgctga aagcgattcc
    97561 ggacgatttg tatgaagcct cagcaatgga tggcgcaggt ccattccaga acttctttaa
    97621 gattacgctg ccgctgctga ttaaaccgct gacgccgctg atgatcgcca gctttgcctt
    97681 taactttaac aacttcgtgc tgattcaact gttaaccaac ggcggcccgg atcgtcttgg
    97741 cacgaccacg ccagccggtt ataccgacct gcttgttaac tacacctacc gtatcgcttt
    97801 tgaaggcggc gggggtcagg acttcggtct ggcagcagca attgccacgc tgatcttcct
    97861 gctggtgggc gcgctggcga tagtgaacct gaaagccacg cgaatgaagt ttgattaagg
    97921 gagataacaa aaatggcaat ggtccaaccg aaatcgcaaa aagcacgttt gtttattact
    97981 cacctgctat tgctactttt tatcgcggca attatgttcc cgctgctgat ggtcgtcgcc
    98041 atctcgctgc gtcaggggaa cttcgcgacc ggtagtctga tcccggagca aatctcctgg
    98101 gatcactgga aactggcgtt aggttttagc gtggaacagg ctgatggtcg cattacgcca
    98161 ccgccattcc cggtactgct gtggctgtgg aactcggtaa aggtcgccgg gatttccgcg
    98221 attggcattg tggcgctctc caccacctgc gcctacgctt tcgcccgtat gcgctttcca
    98281 ggcaaagcga cgctgctgaa aggaatgctg attttccaga tgttcccggc agtactttca
    98341 ctggtcgcgt tgtatgcgtt gtttgatcgt ctgggtgagt acattccatt cattggcctg
    98401 aatactcacg gcggcgtaat tttcgcgtat ctgggtggga ttgcgctgca tgtctggacc
    98461 atcaaaggtt atttcgaaac catcgacagt tcgctggaag aagcggcggc gctggatggt
    98521 gcgacaccgt ggcaggcctt ccgccttgtc ctgttgccgc tgtcagtacc gattctggcg
    98581 gttgtgttca tcctgtcgtt tatcgctgcc attactgaag ttccggtcgc gtcgctatta
    98641 ctgcgtgacg taaacagcta caccctggcc gtggggatgc agcaatacct caacccgcaa
    98701 aactacctgt ggggtgactt tgccgccgct gccgtgatgt ctgcattacc gatcaccatc
    98761 gtcttcttgc tggctcaacg ctggctggtc aacggcctga cggcaggtgg tgtgaaaggt
    98821 taaagatgtt gttctgccaa tgttatgccg ctgcaccctc aacttacgtt atcccaactt
    98881 gtgactgtta ttcggcgctc cacggagcgc ctttttttac tcccgcttca gccgtttcga
    98941 attacacaac cacagggtga tcaccagtaa caggatcgct gccgagtaga tcaacacatc
    99001 cagtggcgat ttatgatcga cgatgatcaa gcgcacaatg gcggtgatcc caatatagac
    99061 aaagtaacgt agcgggaagt gaaaaccgga ctgaaagtac ttcacaatca gcgcgataaa
    99121 ttcgaaatag agaaagtaaa ccaccagccc ttctaccagc tcatatttac tggtttgttc
    99181 tggcacgaac agcacatcag ccagatgcac cgtttctttg ccgaggaaga caaccaaaat
    99241 caggccgagg cacaacaggc caagattgag tacggtctgc aaaatggtgg atataaactc
    99301 cacgcgcgga cgagagagtg acgtcatagc agtacctcct tcacagtggc gagcttcctg
    99361 tataacgcaa aaatgtgacg gagatctata ttttttgtgt atgttttgtt cagctcaatt
    99421 aagtgggcat ctcccatcaa cgtcgcatct ggcaaaacac acagccaaaa aaatcaccca
    99481 ttcaggtgat tccctgccac cagcaatgcg cgaaaaatcg acaatgttcc tctggaacaa
    99541 atgaaacgta acctgataaa ggtggtaaaa atgaagcctt attttgctgc tttgatgtta
    99601 tcagtctctg ttcttcctgc ttatgctggt ctgctcggta ctgctgataa agccgaccta
    99661 ccgcaaagca atgtctccag cccaatgatg gcccagtcgc tcaggcaacc agacctgcaa
    99721 ccgatatcga cggacaggaa gacagaatgt tttcgccttt acacaccaga tcgcaaaccg
    99781 ggggtgaact gcgttcctga tggctcgaca ggctattaag aatgaaggtg ccagtgagtg
    99841 cgaaaactgg cacctttccc atgacttagc ggaaattgac gctacggtcg ctggtcatat
    99901 catacaactg gaacttacgc ccaagttgct gaccaccgtc acgcgtcagc ggcgtccagc
    99961 caatcgccgc acggctgcgt gtcgggccag acgagaagag atccaacggt acagagacat
   100021 acacgccttt ggtgaagtcc ccttcgccgt actcctcttt cgaaacgttg gtgatcgtgg
   100081 catagccgcc aaccaccacg ccgctgtcaa agcgtttagc gatctccagc gtgccgcctt
   100141 tatccccagc cagatactgc ccgacgctcg ctttcactaa cacgtcctgg gcgaaagatg
   100201 gcgtccagta cgcggtcaga tgtccggttt tcacgctgta atcggtgaat ttcatcatat
   100261 cttttgcgct acgccagtcg cgctgtttaa cgtagttggc atccagacca aacgcccagt
   100321 tgctatccag cgggcgatac aacacttctg cccccacgcc gccaaacatg gtttcgagat
   100381 aacctccgta gacctgaccg tagaagccgt tgcccagatg ctggaagtag ttggcttgca
   100441 ggttattcac atagacatca ttctgcacat actcgcgcac atgggtacgc acgcgcggta
   100501 agtgcgagtc ctgcggtgga ttggtgtagt taaacttgtc gtagttgttg gcaaggttag
   100561 caaacaggct gccggtggtc agcagatggt ccgtcagcca caaatccgcc gttcccatca
   100621 cgcccagctg atacatgtaa aagttttccg ggccaccgac cgactggttc agcaccggat
   100681 cgatatggaa atcaaagcgt gatttgtcga tataccagcc ctgctcggtg gacttcggaa
   100741 ccactggctc gacgcgtttt tgcgccagcg tcgtttcgtg gcccaacggc tctccgccga
   100801 gatgattttt caggctggcg acatcggttt ccgtcgtcac ctgcggcatg ttaaggcgat
   100861 tttccgtaat gcggatcgtt ttgatcccat ccggcagatc gttcatcacg atccgattag
   100921 cacggatgat cccttcgcgc gaatcacgat atttcacctg ctcgccggta acatacagcg
   100981 tatcgccttt cgcctggatc tgcggatcgg ccagtccggc gttgtatttc aacagcgtta
   101041 actgattcgc caccaccgag tgctgcaaaa tggcatcctg cggttgcggc tggtatttcg
   101101 gtcgggcgtt atcgttgtag gacgggcgca gatcgttaaa gttggtgcgc agcgtgacgc
   101161 caaacataaa ggtgttgcca cgttcatagc taaggttaac gtcggcccaa tcggtaacgc
   101221 gataaatcgc cccgacgtta aacttacttt tttgctccag cttcccggca aaatcctgct
   101281 gataattatt accttcatac tccagtttca ggcgcagcgg ttgccagggc gtctggtatt
   101341 ccacgccgcc aaacagtgag gccgggccgt ggaacatctg actgccgtcg atagatcccg
   101401 cttgtttgta gctgttatcg cgatagcaat atttatcact ggctgaacag agcggatttt
   101461 tcacattacc gctggtgccc agatatcccc atcccaggcc gagcgtaaaa tcgaacggcc
   101521 cccaggcttt gctggcaaca agatattccg catcaaacag ccccgtaccg ccgatatccc
   101581 gcgcaccaac cgccacttgc ggcagccaat aactctcttc ccacaaacgc agtttgagat
   101641 cgaaggcttt atctttatac gtttgatcgc cagagaacgc ttcgacgctg ctgtactgcc
   101701 gggtgcgcac gtcggtgtag cgcagcgttg tttccagcca cgggaagagt tgcactgaag
   101761 ctgagtaata acggtactga tcgttatcgc gatagttcag gctcagctcc ccttcccgcg
   101821 ccatgcgcgc ggtgggcgtt tgtaataatc ctacgccacc gaaatccgat tgcgacggac
   101881 caatgggcgc aggatatgtt tcgccgtagc aagctgtgct aatgcccagc gccagtaagc
   101941 taagcagatg tctttttttc attattgggg tatccgctgc gtcagagtct gaagaatgtc
   102001 ggcgttaagg gcatcaggtg tctcactcca gacggagtcc gcgaggccaa cataaataat
   102061 gctgccgggc atcggctcta cgtgacgctt gttccaataa gccaccggcg ctttttgcgt
   102121 gcgtccgtcc gggtaaacca cccacgcgta gctgcgatcc gcaccgctga gcaggctctg
   102181 gtcagagaga tagctcgcca cgtcgcgacc tagagtgaat gactgcttgc caggacgact
   102241 gataagcccg aacaacgtga ccgtggacgg cggcgttccg acccacagcg tgtagttgcc
   102301 ctgcaacggc gggttaccgc gttcggcaac gcggacgata tcaggatcaa gattgatttt
   102361 ttgcctgccc gtcaccttca acgcctgaat ttgctggcgt aaggcgttaa tcgcggcggc
   102421 atcgtcggcg ctggaatctg cagccagttc tgccagccgt gtcagcaacg cctgctgctg
   102481 acgcaatgca gccgccgtgg ccagctcttc gctaatcacc gctccgggcc accagctgtt
   102541 tgccagccgc ggttggccta ccagatcgat taaatgctct gcgcccgtca gcgttttggc
   102601 ctcactgctg ccattgctga acaccttgac ggttcccgcc gcgaagactg atgacgcccc
   102661 cgcactcaaa agcaacgcga caatagtttg tttaatcatt gttttgccgc cttgatgagg
   102721 gtggttttca ccgggaagta atccgcgccg agatactgtt ccgactggcg aatttgccct
   102781 tcgcgtcgat ccaatagcgg ttatgccagc gcgcctggtc agtgaagact tcttcgtcca
   102841 gcacgcgaac cggggtttca tcgctgccga ctttgacggt atcggtgcca ttccatttga
   102901 agactgagcg cgcggtggcg tagcgtacct gctggtactc ggtccagccc atcgtgcgcg
   102961 tccaggttgc accatcaatg atttgtgcgg gtttaatcag cgggtcggcg gcaaggttat
   103021 tcacttcaat caggttgtcg ccgccaagca aagtcttcac cagacgacca tgttgtgtga
   103081 caagggtggc ctgatcctga gtgacccatt tttgttgtcc gtcttcagcg aaggccagca
   103141 ccacaaataa ctgcggcccg ccattaagct gcatgtactg gctggcgtag ggcatatttt
   103201 gaatatcatc gtccgtcagc tgtacgcctg gcgtaccgaa cagactgtcc catagtgaat
   103261 tgcccagctc tttagtcgtg gctgaacagg cctgtaatag caggcagata agaatgagtg
   103321 caggtcgctt cacgactctt ctccagaggg gcagaataac cacaccggag tgtggttatg
   103381 gttaatcccc ttcatacttc aagctgcaga tgcggtgacc gcactcgttc accccggtca
   103441 catagttatc tatgctcccg gggactcact cgcttgtcgc cttcctgcaa ctcgaattat
   103501 tttggggata catactttat tactgggtac tggtggtcgt ggttgtggtg gtcccggtat
   103561 tagaaccatc accgccaccg gttgccgcga gcgcgacacc caccgccgag cttacggtgc
   103621 tgacgctggt cgcggaggaa ctacccgccg aaaccgacgt tgctgccgat cctgccgctt
   103681 cgcccacctg tacaggtgca gcccacgcag aagtcgccgc aagcgcagat atggcaaaaa
   103741 tgccatacag aacttttttc ataacaattt cccttcattg aatgaatgga gatttaccca
   103801 aaacatttcg ggcgggatcg agtatacaca actaaagcat gcgaattacg gcgcggggaa
   103861 ttagtgatgg agttttagga caattggaat gaatatcaaa tataagcttt gacttatata
   103921 taaataagat taaaaatcat gaagttaaaa taaaataaaa agtaattact ctgcgtattt
   103981 tccgaaataa accacgatta aaaacttatt ttaggattaa cctcacggta tgattttcgt
   104041 taaatgacag acaagaggat ttatctgata aaacaatgcc gacttatagt cggcattgtt
   104101 tttaaggcta tttaatcaat cgtactatgt attaattgcc gaatacgatg tacatatcgg
   104161 catcgacctg taggcctgat aagacgcgac cgcgtcgcat caggcatcgg ttgccggatg
   104221 cggcgtgaac gccttatccg gcctacatat cgacaatgat taaccgcgcc acgctttata
   104281 gcggttaatc agaccattgg tcgagctatc gtggctgctg atttctttgt catctttcag
   104341 ctctggcaga atacggttcg ccagctgttt acccagttcc acgccccact ggtcgaaggt
   104401 gaagatgttc aggatcacgc cctgagtaaa gattttgtgc tcatacagcg caatcaacgc
   104461 acccaggctg aacggagtga tttcacgcag caggatggag ttagtcgggc ggttaccttc
   104521 gaacactttg aacggcacca cgtagtcaag cgttgccgga tctttaccct gatcgcgata
   104581 ttcctgctca accacttcgc gggatttacc aaacgccagc gcttcggtct gagcaaagaa
   104641 gttagacagc agtttctggt gatgatcaga gagcgggtta tgggtgatag ccggagcgat
   104701 gaaatcgcac ggtaccattt tggttccctg gtggatcagc tgatagaacg cgtgctgacc
   104761 gttcgtgcca ggttcacccc agataatcgg gccagtctgg taatccacaa ccttaccgtt
   104821 acggtcaaca tacttaccgt tggattccat attgccctgc tggaagtacg ccgcgaaacg
   104881 gtgcatatac tggtcatacg gcagaatcgc ttcagtttcc gcgccaaaga aattgttgta
   104941 ccagatgcca atcagcgcca gcagtacagg caggtttttc tcggcaggcg tggtggagaa
   105001 atgcttgtcc atcgcgtgtg cgccagaaag cagttcaacg aagttatcaa agccgatgga
   105061 gagaacaatc gacaggccaa tcgctgacca caaagagtaa cgaccgccga cccagtccca
   105121 gaactcgaac atgttggcag tatcaatacc aaactcgcca acggctttgg cgttggttga
   105181 gagcgccgca aagtgtttcg caacgtgctt ctcatcacct gccgctttca ggaaccagtc
   105241 acgcgcgctg tgggcgttgg tcatggtttc ctgagtggtg aaggttttag atgctaccag
   105301 gaacagcgtg gtttccgggt ttactttttt cagcacttcc gcgatgtgag ttccatcgac
   105361 gttagaaaca aagtgcatgt tcaggtggtt tttgtatgga cgcagtgctt cagtcaccat
   105421 gtacgggccg aggtcagaac cgccgatccc gatgttcact acgtcagtga ttgccttgcc
   105481 ggtatagcct ttccactcac cggaaataat cgcttccgag aaggttttca tcttctccag
   105541 caccgcgttg acttccggca taacatcttt gccatcaacc agaatcgggg tattgctacg
   105601 gttacgcagc gctacgtgca gtacggcgcg gttttcagtg cggttgatct tctcgccaga
   105661 gaacatcgac ttaatcgcgc ccgccagatc gcactctttc gccagatctt gtaatttcgc
   105721 cagcgtctct tcagtgatgc ggtttttgga gtaatccacc agcatctgat cgtcgaaggt
   105781 tgcggagaac ttagaaaaac gatcgccgtc tttagcaaaa agatcggcga tcgtaacgtc
   105841 tttcatttca tcgaagtgtt tctgtagtgc ctgccaggca gcggtctgcg ttggattgat
   105901 gtttttcatt agcaatactc ttctgatttt gagaattgtg actttggaag attgtagcgc
   105961 cagtcacaga aaaatgtgat ggttttagtg ccgttagcgt aatgttgagt gtaaaccctt
   106021 agcgcagcga agcatttatt agctgaacta ctgaccgcca ggagtggatg aaaaatccgc
   106081 atgaccccat cgttgacaac cgccccgctc accctttatt tataaatgta ctacctgcgc
   106141 tagcgcaggc cagaagaggc gcgttgccca agtaacggtg ttggaggagc cagtcctgtg
   106201 ataacacctg agggggtgca tcgccgaggt gattgaacgg ctggctacgt tcatcatcgg
   106261 ctacaggggc tgaatcccct gggttgtcac cagaagcgct cgcagtcggg cgtttcgcaa
   106321 gtggtggagc acttctgggt gaaaatagta gcgaagtatc gctctgcgcc cacccgtctt
   106381 ccgctcttcc cttgtgccaa ggctgaaaat ggatcccctg acacgaggta gttatgtctg
   106441 aaattgttgt ctccaaattt ggcggtacca gcgtagctga ttttgacgcc atgaaccgca
   106501 gcgctgatat tgtgctttct gatgccaacg tacgtttagt tgtcctctcg gcttctgctg
   106561 gtatcactaa tctactggtc gctttagctg aaggactgga acctggcgag cgattcgaaa
   106621 aactcgacgc aatccgcaat atccagtttg ccattctgga acgtctgcgt tacccgaacg
   106681 ttatccgtga agagattgaa cgtctgctgg agaatattac tgttctggca gaagcggcgg
   106741 cgctggcaac gtctccggcc ctgacagatg aactggtcag ccacggcgag ctgatgtcga
   106801 ccctgctgtt tgtcgagatc ctgcgcgaac gcgatgttca ggcacagtgg tttgatgtac
   106861 gtaaagtgat gcgtaccaac gatcgatttg gtcgtgcaga gccagatgta gccgcgctgg
   106921 cggaactggc cgcgctgcag ctgcttccac gcctcaatga aggcttagtg atcacccagg
   106981 gatttatcgg cagcgaaaat aaaggtcgta caacgacgct tggccgtgga ggcagcgatt
   107041 atacggcagc cttgctggcg gaggctttac acgcatctcg tgttgatatc tggaccgacg
   107101 tcccgggcat ctacaccacc gatccacgcg tggtttccgc agcaaaacgc attgatgaaa
   107161 tcgcgtttgc cgaagcggca gagatggcaa cttttggtgc aaaagtactg catccggcaa
   107221 cgttgctacc cgcagtacgc agcgatatcc cagtctttgt cggctccagc aaagacccac
   107281 gcgcaggtgg tacgctggtg tgcaataaaa ctgaaaatcc gccgctgttc cgcgcgctgg
   107341 cgcttcgtcg caatcagact ctgctcactt tgcacagcct gaatatgctg cattctcgcg
   107401 gtttcctcgc ggaagttttc ggcatcctcg cgcggcataa tatttcggta gacttaatca
   107461 ccacgtcaga agtgagtgtg gcattaaccc ttgataccac cggttcaacc tccactggcg
   107521 atacgttgct gacgcaatcc ctgctgatgg agctttccgc actgtgccgg gtggaagtgg
   107581 aagagggtct ggcgctggtc gcgttgattg gcaatgacct gtcaaaagcc tgcggcgttg
   107641 gcaaagaggt attcggcgta ctggaaccat tcaacattcg catgatttgt tacggcgcat
   107701 ccagccataa cctgtgcttc ctggtgcccg gcgaagatgc cgagcaggta gtgcagaagc
   107761 tgcattttaa tttgtttgag taaacatagt atggcccgga agacattatt tcgggccgta
   107821 ttgtttttct tgtcattatg cccataaata aacgagtcta ttccctgtta accagcgtct
   107881 ttatcggaga ataattgcct ttaatttttt atccgcatct ctaactaatt atcgaaagag
   107941 ataaatagtt aagagaaggc aaaatgaata ttatcagttc tgctcgcaaa cgaattcccg
   108001 tacactgaac tctctggctc actgagggca ccatcatggc actcccccgt attacccaaa
   108061 aagagatgac tgaacgcgag cagcgtgaac ttaaaacact gctggatcgg gcgcggattg
   108121 cgcatggtcg cgtactgacc aactccgaaa ctaacagcat taagaaagag tacatcgata
   108181 aattaatggt cgagcgtgag gctgaagcga aaaaagcccg ccaattgaag aaaaagcagg
   108241 cttataaacc ggatcctgag gcgtcattta gctggtcagc aaatacgtcg acgcgcggca
   108301 ggcgctgatt aacgcccctt ctttttgcgc cccggcgagg taaaacgctt accgttggac
   108361 gccgggcgac cgcctttttc cgccgttttt tccatcttaa cgactggacg tttgatgcct
   108421 accgttttcg gtttggcctt cgctttaggt tttgcctcgg aagaggaatt ttcaatgagc
   108481 ttaaagagat cgattaactc atcgtcggtt aaatcgcgcc actcacccag cggaatgccg
   108541 cttaagctga cgttcataat gcgcgtacgt tccagctttt tcacttcgta accaaagtgc
   108601 tcgcacatgc gacgaatctg acggttcagc ccctgcacca gggtaatgcg gaagacaaac
   108661 ggtgcttctt ttttgacttt gcacttttta gttactgtcc cgaggatcgg tactcccgcc
   108721 cccataccac gaataaattc atcggtaatc ggtttatcaa ccgtcaccag atactctttc
   108781 tcatgatcgt tacccgcacg gaggatctta ttcaccagat cgccgtgatt ggtgaggaaa
   108841 atcagcccct gggaatcttt atccaggcgg ccaatcggga acacgcgttt gctgtggtta
   108901 acgaaatcga caatgttatc gcgctcgcca tcttcggtgg tgctgacaat accgacgggc
   108961 ttgttcaggg cgataagtac caaatcttcg gattcccgag gctcaatcaa ctgaccgttt
   109021 actttcacaa cgtcgccggg tttcacctga tcgccaatgg tggctcgctt gccattaagg
   109081 aacacattgc attgctcgat atagcgatcc gcttcgcggc gtgagcaaat tccgctttcg
   109141 ctgatgtatt tatttaaacg gactgatgag tcgggcagca taaattctcc tgtaaaacgc
   109201 ggaatatacc gtacctctta gcggggaaaa acagggttat gcggcaacgt cgaaattaaa
   109261 tgtgatctgc cacgcctttt atcacaaaag tgtctccgga agtacgatgc aaatttgcct
   109321 ggcaaaataa gaataatcaa caaattacga atcgctgcac acctgtgctg atgactacta
   109381 cgcagagaaa caaatgtgca agagaaagcg ctatttcccc ccatgcaaag agtgaaaaat
   109441 cgctttgaaa ggccatcatg atggggttat atcagatcaa gcggtgagaa cgcgtatttg
   109501 cacaaatgtg cagaaccagg aggcggcaat ggaaaacagt gacgatatcc gtttgattgt
   109561 gaagattgcc caactctatt acgaacagga tatgacgcag gcgcaaattg cgcgcgaact
   109621 gggtatttac cgcaccacca tcagccgctt gcttaaacga ggccgcgatc agggaattgt
   109681 caccatcgcc atcaactatg actacaacga aaatctctgg ctggagcagc aagtgaagca
   109741 aaagtttggc ctgaaagacg ttgtggtggt gtcaggaaat gatgaggatg aagatactca
   109801 actggcgatg atggggttac acggcgcgca actgctggat cgcttgctgg aacctggcga
   109861 tattgtcggt ttttcctggg gccgcgcggt gagcgcactg gttgaaaact tgccgcaggc
   109921 ggggcaatcg cggcagttaa tttgcgtgcc gattattggc ggcccgtccg gtaaactcga
   109981 aagccgctat cacgtaaaca cattaaccta cagcgcggca gcgaagctga aaggggaatc
   110041 gcatctcgcg gattttccgg ctctgctgga taacccatta attcgtaatg ggatcatgca
   110101 gtctcagcac tttaaaacca tctctgccta ctgggataat ctggatgtcg ccctggtggg
   110161 aattggctca ccggccattc gcgacggcgc taactggcat gcgttttatg gtggtgaaga
   110221 gagtgacgac ctgaatgccc gccaggttgc tggcgatatt ttctcgcgct tttttgatat
   110281 tcacggcgca atggttgaaa cgaatatgag cgaaaaaaca ctctctatcg aaatgaataa
   110341 attaaagcag gcacggtatt ccattggcat tgccatgagc gaagaaaaat acagcggaat
   110401 tgttggtgca ctgcgtggaa aatatattaa ttgtctggta acgaatagca gcacagctga
   110461 actattactg aaataaaaat cttcccgatg tttaccgtaa taaaaattac ggcgggtccc
   110521 tattatctaa aatcaggagt ctgttatgca aacgtggtta aatttgcagg ataaaatcat
   110581 tattgtcacc ggtggcgcat ccggcattgg tctggcgatt gtggatgaat tattagcaca
   110641 aggcgcgaat gtacagatgg tcgatattca ctgtggcgat ggtcagtatg aaagccataa
   110701 aggttatcag ttctggccga ccgatatttc cagcgccaaa gagataaatc atacggtagc
   110761 agaaattatc cagcgttttg gtcgcatcga cggtctggtt aacaacgccg gggtcaattt
   110821 cccgcgtctg ctggtcgatg agaaagcgcc tgccgggcag tatgaactca acgaagctgc
   110881 attcgaaaaa atggtcaata tcaaccagaa aggcgttttt ctgatgtcgc aggcggtggc
   110941 gcgacagatg gtcaaacaac atgatggcgt gattgtgaat gtttcctcag aaagtgggct
   111001 ggaaggctca gaaggccaaa gctgttacgc cgcgaccaaa gccgcgctca atagcttcac
   111061 gcgctcctgg tcgaaagaac tgggtaagca cggtatccgt gtggtcggta tcgcgccggg
   111121 gattctggaa aaaacaggac tgcgcacgcc ggaatatgaa gaagcgctgg cgtggacgcg
   111181 caatatcacc gtcgagcagc tgcgtgaagg ctataccaaa aacgccattc ctattgggcg
   111241 cgccggaaga ttagcagaag tggctgattt tgtttgttat ctgctgtctg aacgcgccag
   111301 ctatatcacc ggagtaacca ctaacattgc aggcggcaaa acgcgcggct aaggaggctt
   111361 tatggtcaat gccatttttt gcgcccacgg caaattagcc tgcgccatgc tggaatcggt
   111421 tcagatggtg tacggcgatg ccagggtcga ggccgttgaa tttgtgcccg gcgagaacgc
   111481 cggagacatt gtggcaaagc tggataagtt agtaagcact cacaatcatg acgagtggct
   111541 aatcgccgtg gatttacagt gcggcagccc gtggaatgcg gcagcgatgc ttgcgatgcg
   111601 caatcctcgt ctgcgggtaa tcagcggcct ttctctgccg ctggcgctcg aactggttga
   111661 taaccaggac agcatgaata ttgatgaact gtgtgaacac ctgacgcaaa tcgcaaagca
   111721 aacctgcgtc gtctggagac aggtggcaac agcggaggag gatttctgat gaacattacg
   111781 ctcgcccgca ttgatgaccg cctgatccac ggccaggtca ctaccgtctg gtctaaagtg
   111841 gctaacgccc aacgcattat tatctgcaat gacgaagttt ataacgatga agttcgccgg
   111901 acattattgc gtcaggctgc gccaccggga atgaaggtta acgtggtcaa tattgaaaaa
   111961 gccgtcgcgg tttatcacaa cccgcaatat caggacgaaa ccgtttttta tcttttcact
   112021 cgcccacagg atgccttagc aatggttcgc cagggggtaa aaataggtac cctgaatatt
   112081 ggcggtatgg cctggcgtcc tggtaagaaa cagttaacca aagcagtttc attagatgac
   112141 gatgatatta acgcatttca tgagttaaat aatcttggcg tcatactgga tttacgcgtc
   112201 gtagcgtcag atccctcaat caatattatc gacaaaataa acgaacagtt aatcgcgaat
   112261 taaataatag cgcctgtgta catatgcttt aacaggcagg gatgacgaca cttaaaaggt
   112321 gacaaatcat ggaaataagt accctacaaa tcattgctat atttcttttt tcctgtattg
   112381 ccggaatggg cagcgtgctg gatgaatttc agactcatcg tccgttaatt gcctgtacgg
   112441 tgattggttt aattctcggt gatttaaaaa ccggaattat gctcggtggt acgctggaat
   112501 tgatagctct cggctggatg aacgtcggcg cggcgcaatc tccggattct gcactcgcca
   112561 gcataatctc cgccattctg gttatcgttg gtcagcaaag catcgccacc ggaatcgcca
   112621 tcgcgttgcc tgtggctgct gcaggccagg tgctgacggt gtttgcccgt accatcaccg
   112681 tagtgttcca gcacgcggcg gataaagcag cggaagaagc acggtttcgc actctcgata
   112741 ttctgcatgt ctccgcgctt ggcgtgcagg cactgcgcgt tgctattccg gcactgattg
   112801 tctcgctgtt cgtcagcgct gatatggtga gcaatatgct gagcgccatt cccgaatttg
   112861 tgacccgtgg actgcagatt gctggcggtt ttatcgtggt ggtcggttac gccatggtgc
   112921 ttcgcatgat gggcgtgaaa tatttgatgc ctttcttttt cctcggtttc ctcgcaggtg
   112981 gctacctcga tctcagtctg ctggcgttcg gtggcgtcgg cgtgatcatg gccctgctct
   113041 acatccagtt aaatccacag tggcgtaaag ctgaaccaca tccccagacc accactatca
   113101 ccgcccttga ccaacttgat gattaacgga gccatcatgg aacagagaaa aattacacgc
   113161 agcgatctgg tgagcatgtt tctgcgctcc aacctgcaac aggcatcctt taactttgaa
   113221 cgtattcacg ggctgggttt ttgctacgac atgatccccg ccatcaaacg actttaccca
   113281 ttaaaagagg atcaggttgc ggcgctcagg cgacacctgg tgttcttcaa taccacgcca
   113341 gccgtatgtg gcccggtcat cggcgtcact gctgccatgg aagaggcgcg ggccaacggc
   113401 gcggaaattg atgacggtac cattaacggc atcaaagtcg gtctgatggg accattggca
   113461 ggagttggcg atccactggt ctggggaacg ctgcgcccga ttaccgccgc gctcggcgca
   113521 tctctggcac tttcgggcaa cattctcggc cccctgctgt tcttctttat tttcaacgcg
   113581 gtgcgtctgg cgatgaagtg gtatggccta cagctcggct ttcgcaaagg ggtgaatatc
   113641 gtcagcgata tgggcggtaa tttgctgcaa aaactcaccg aaggcgcgtc gattctcggg
   113701 ctgtttgtga tgggcgtgct ggtgaccaaa tggacgtcaa tcaacgtacc gttggtggtt
   113761 tcacaaacgc ctgccgccga tggtgccacc gtcaccataa ccgtgcagaa catcctcgac
   113821 caactttgcc ctggtttgct ggcgctcggt ctgacgctgc tgatggttcg tctgctcaac
   113881 aaaaaaatta acccggtatg gctgattttc gccctgtttg gtttagggat tatcggtaat
   113941 gcgctgggct tcctgtcctg attcttcgcc ccggcacgac tgccggggtc atcgctcaac
   114001 atgaggtggt ttatgaaaac aacagctctg cgtctttatg gcaaacgtga cctgcgtctg
   114061 gaaacctttg accttcctga aatgcaggag gatgaaatcc tcgcgacggt ggtcactgac
   114121 agcctgtgcc tctcttcctg gaaagaggcc aatctgggtg aaaaccataa aaaagtaccc
   114181 gacgatgtgg cgaccaatcc catcatcatc ggccacgaat tttgcggcga tattctggcc
   114241 gtgggtaaaa agtggcaaca caaattccag ccgggtcagc gttatgtgat tcaggccaac
   114301 ctgcaactcc ccgaccgccc ggattgcccc ggctactcct tcccgtgggt aggcggcgaa
   114361 gcgacgcatg tggttattcc caacgaggtc atggaacatg attgcctgct ggcatacgac
   114421 ggcgaaacct attttgaagg ctcgctggtt gaaccgcttt cctgcgtgat tggcgcgttc
   114481 aacgccaact atcatcttca ggaaggtagt tataaccaca caatggggat tcgcccgcaa
   114541 gggcgcacgc tgatcctcgg cggcaccggt ccgatggggc tgctggcgat tgattatgcg
   114601 ttgcatggac cggttaaccc gtcgctgctt gtcattactg ataccgacaa cgacaaattg
   114661 agttatgcgc gcaagcacta tccatcagaa ccgcaaacac tgattcatta tctcaatgcc
   114721 gccgatgcag catttgatac gttaatggca ctgagtggcg gtcacggctt cgatgatatt
   114781 ttcgtctttg tgcctaatga aggactggtg actctcgcct cttccttgct ggcgacagat
   114841 ggttgtctga atttcttcgc cggaccgcag gataagcact tcagcgcgcc aattaatttc
   114901 tacgatgtgc attatgcatt tacccactac gtgggcacat caggcggcaa taccgacgac
   114961 atgcgcgcag cggtcaaatt gattgaagag aaaaaagtgc aggccgcaaa agtggtaaca
   115021 catattcttg ggctgaatgc cgcgggcgaa accacgcttg aattgcctgc catcggcggc
   115081 ggcaaaaagc tggtgtatac cgggaaatac ctgccgctga cgtcactcac gcagattcag
   115141 gatcaagcac tggcggtgat tctggcgcat catcagggga tctggtccgg tgaggcggag
   115201 caatacctgc tcgcccatgc agaggcaatt tcccatgatt aatcgcgcta cttaactctg
   115261 catgtcgctg gctggtcgcc gcggcaactt cgcgacctga tgtgcggctg attgctgaag
   115321 cggcacaatg tgcacgagaa gaataaaaaa acatcagcgc cttaaggggc gctcaaattg
   115381 gtggcaaagt gcacattgtt tatgccggat gcggaatgaa gccttattcg cctacataat
   115441 cttgaaaatt caatatattg cagttatctg gtaggccagg taagcgtaac gcatcaggca
   115501 atcctgtgtt tgtcatcagt ctcagcgcct gtgcaatatg tgacatcgtg agccatgtca
   115561 gtaagtggtt aaattcgcag tggtagcatt ccctccgtgt tttcttacta accggagaag
   115621 taatggaact gcttttattg agtaactcga cgctgccggg taaagcctgg ctggaacacg
   115681 cgctgccgct gattgctgaa cagttgcagg gtcgccgttc tgcggtattt attcctttcg
   115741 ctggcgtaac gcagacctgg gatgattaca cagcgaaaac ggctgcggtt ctcgctccgc
   115801 tgggtgtttc tgtcaccggt attcatagcg ttgtcgatcc cgttgccgcg attgaaaatg
   115861 cggaaattgt gattgtcggc ggcggtaata ctttccagtt gctgaaacag tgccgcgagc
   115921 gcgggctgct ggcaccaatt actgacgtgg tgaaacgtgg cgcgctgtat attggctgga
   115981 gcgcaggcgc taaccttgct tgcccaacca ttcgtaccac caacgatatg ccgattgtcg
   116041 atccgcaagg tttcgatgcg ctaaatctgt tcccgctgca aatcaacccg cacttcacca
   116101 acgcgctgcc ggaaggccat aaaggtgaaa cccgtgagca gcgtattcgc gaactgctgg
   116161 tcgtcgcgcc agaactgacg attattggtc tgccggaagg taactggatc acagtaagta
   116221 aaggtcacgc tacgctgggt ggcccgaaca ccacttatgt atttaaggct ggtgaagaag
   116281 cggtgccgct ggaagcgggt caccgttttt aagtgaattg ccggatacat gatccggcat
   116341 cttgttcata gtgcctgatg cgacgcttta cgcgtcttat caggccttca gggtcacggt
   116401 attaatactc gtcccgtcct tcatcttcat ccggctgttc cagcacactg taagccaccg
   116461 aacaaaacag cgagttcaga cgctgcatat ctcccagtaa gcctaaatgt agcgaactgg
   116521 tttcaatact ctgcacgttt tgctggtgca ggcgatcgac gtgggcgtgg gaatagcggc
   116581 gattaagaat gcgaaaacga tgtttgctgc ggcgcaaacg acgggcgctg gtaacatcgc
   116641 cagagaagaa aaccgacatt gccagtttta aattactgag caattgctca tagagcgcat
   116701 ccagttcctt caagccatca agcgaaaatg ctcgccttgc tgccagcgat ttgtcggcaa
   116761 tttcactccc catgcgctcg acgatatcgg aggcctgttc aaggttgagc gacatttcga
   116821 tgatctccgc ccagcggcgc gactcttctt ctgccagttc ctcttttggc atccgcgcca
   116881 gatacagctt aatggcggta tagagaacgt tgatgtcatc agccagctta cgcagctctt
   116941 tctcctgccg tggctcgccg tgcatcactt tattcaaccc ttccatcatc tgttccatgg
   117001 cgtcgccaat gcgcagggtt tcgcgggcag cgttcgccag agcaagcgtg ggcgtatcca
   117061 gcgcgctgac atccagatgt ttaggccgta gctgggtatc cagttccggt tcatcgcgaa
   117121 taatcgtttt gcaaaaccgt gccatcgggt caacgaatgg caacatgacc aggcagcgta
   117181 caaggttgta gaagacgtgg aaatagatca ccaattccgc ttttggcaat ggcaacttcc
   117241 ccattgtctc tgccagcaaa tgaacaaacg gcaggataat caggctaccc accagcttaa
   117301 acagcaggct acccagcgcg acacggcggg ctgcggcatt ggcggcactg ttgttgagca
   117361 tcgccagcag gccggaaccg aggtttgcac caatcaccag acaaagcgcc actgggaaag
   117421 agataatgcc tgcggcggtc agagtcgcgg tcagcagtac cgcggcaagg ctggagtagc
   117481 tgataatggc gaacatcgca ccaatcagcg catccagcaa aatgtcgcct gtcagcgagg
   117541 caaagatcac ctgaacgccg tttgcctgag tgatcggcgt tacggcctgc acaatcaact
   117601 ccagcgccag taaaatcagc ccaagaccaa taccgacgcg gcccagctgc ccggcgcgtg
   117661 actgtttgcg tccgaggaaa aaaatcacgc cgataaaaat aagtaacggt gacagccagg
   117721 ataaatcgaa ggtgagaata cgcgccatca gcgccgtccc gacatctgca cccagcacaa
   117781 tcaccagcgc aggcgcgaga gcaaccagat cctgagcgac gaacgaggtc accagcatgg
   117841 tggtggcatt actgctctgc accagtgcgg taacgccgat ccccgcgcaa aaggcgagcg
   117901 gctttttttc gacgctccgg ctaaggacag tacgcagacg cgcgccgaag acgcgcatta
   117961 cgcctgttcg aacaatatga gtcccccaga ccagcagggc gacggcagaa agcaggtgaa
   118021 gcaaagttag cacggaatca ggttctcctt tttgttatgc attttcctgt taagattttt
   118081 ctaatcacgt tgtttttatc caggcatttc tcttgtatgt actttcagta taagagattt
   118141 atacaagaaa gcatttaaaa aggcatttat ataaaaatcc acctgtatct gttgctattt
   118201 ctgtcacact ttcccctgtc gtttacacca gataatcgtt gtcacttttg tgacctctga
   118261 gaagataaac aacgaaatat ttaagaccag atcctgtgaa taaaaataat taagtaacaa
   118321 cggtattacg gagattgaac agcttacggt agcaaaacgc ataaactcct gaatcgccaa
   118381 aaaagagaaa ctaaataatt tttgtcttaa aaaacattgc gcagaaaaga ataaagagaa
   118441 caataatact atttgtagcg taacagtggc gactgcataa gcgtaaaaac cgccttcaac
   118501 accgatatcc ttaagtaaag aaagtgaatg aacgatatcg gaataaaaag ggatacgacc
   118561 attaccaagc gagtagaaca gatatgtggc tatcgcgata acatccataa caccccaaaa
   118621 aagaagaatg tattttttat attgacgttg cataaagtaa atccttttta cgaaaacaaa
   118681 tattaccagg aattattata attgcgggcg tttttggatt aacactccac ccgtattttt
   118741 taaacctctg tcagctatac cttcactaaa caagacatcg atacgccctt tacttttatc
   118801 gtgatacaga atactgcatg agatacacct tataccctct ttggatttac gaaaatctaa
   118861 ccaataacga ccatcataaa atacccactc cacacctgtt tttatattaa ttatccaatc
   118921 attataacct gttggttttt ttcctaatag ttcagtaact tcggactctg aacatccttc
   118981 gaaagcaaaa aaatctattt catcaatttt tatcatgttc aataatccat tatttatgac
   119041 attaaaaacc ggggcaaata ccccggtgtt atcacttact cggcatcata ccccagattc
   119101 ggtgccaacc aacgctcaac ttcggtaacg ctcattcctt tacggcgggc atagtcttca
   119161 acctgatcgc gctgaatttg cgccaccgcg taatacttgc tgtctggatg actgaagtac
   119221 caacccgaaa ccgatgcacc cggccacatg gcgaaggatt ccgtgagttt cataccggta
   119281 tgtttttcca cttccagcag ctcccagatt gtggcttttt ccgtatgttc cgggcaggca
   119341 gggtagcccg gtgccggacg gatgccctgg tagttttcgc ggatcagctc ttcgttgctg
   119401 aggttctcgt tcggcgcata gccccagtag actttacgca cacgctcatg gagatactcc
   119461 gcaaaggctt ccgccagacg gtcggcaagc gctttcacca tgattttgtt gtaatcatca
   119521 tgctgtgctt caaaggcatc agccagtgcg tcctcttcca gcccgccagt cacggcaaat
   119581 gcgccgatgt aatctgcttt accagaaagc ttcggcgcaa cgaagtcagc gagacagtag
   119641 ttagcgaagc cggttttttc ggtctgttgg cgtagatggt ggctgacgtt gatcacatgg
   119701 gtacgcgttt cgtcacggta gatttcaatg tcatcaccca cacggtttgc cgggaacagg
   119761 cccaccacgc cacgcgggtt cagcattttt tcggcgctta atttatccag catgtcgttg
   119821 gcgtctttaa acagccgctg cgcctcaaca cctaccactt catcttccag aatgcgcgga
   119881 tacttcccgg ccagcgacca ggtcataaag aacggcgtcc agtcgatgta attacgcagc
   119941 gtttcgatgc tggcttcgac ttcctgcaca ccgagacggt gcgccaccgg cggcgtgtaa
   120001 gcctgccagt caaaagcgaa gtcgttatca cgcgccgctt ccagcgtgac cggtggtgtg
   120061 cgtggtttct ttcgtccgtg ctgaatacgc acggtttcgt actccttgcg ggtacgagca
   120121 acgaaatcat cgcgctgagt gtcggaaagc agcgccgcca ccacaccaac ggtgcgcgat
   120181 gcgttctgca catacaccgt cgggccgctg tagttctgct cgattttcac cgccgtgtgc
   120241 gcttttgagg tggtcgcgcc gccaatcaat agcggaatgg tgaagccctg atgctccatc
   120301 tctttcgcca cgttaaccat ctcgtccagc gacggcgtga tcagccctga aaggccaatc
   120361 aaatcagcat tcacttcttt agcggtacgg agaatttttt ccgcaggcac catcacgccg
   120421 agatcgacaa tttcgtagtt gttacattgc agcaccacgc caacgatatt tttaccgatg
   120481 tcgtggacat cgcctttcac ggtggcgatc accatcttgc cgttggtttt accctgctct
   120541 ttgctggctt caataaacgg ttcgagatac gccaccgcct gcttcattac gcgcgccgat
   120601 ttcaccacct gcggcaggaa cattttgccc tcgccaaaca ggtcgccgac cacgttcatg
   120661 ccgtccatca acgggccttc aatcacttca atcggacgcg tagcctgctg gcgggcttct
   120721 tcggtatcct gctcgataaa ctcggtaatg cctttgacca gcgagtattc cagacgttta
   120781 ttcacttccc acgagcgcca ctccgcctgc tgggtgttag cggtgtcgtc ggttttgctg
   120841 ccgcgatatt tctcggcaag ctcgagtaaa cgctcggtgc catcgtcgcg acggttgagg
   120901 atcacatcct ccaccgcgtc gcgtagctcg gtaggcaggt cgtcgtaaat cgccagttgc
   120961 ccggcgttga cgatccccat atccatgcca ttgcgaatag cgtagtagag gaacaccgcg
   121021 tgaatggctt cgcgcaccgg atcgttgcca cggaatgaga aagaaacgtt agaaacgccg
   121081 ccggaaatca gtgcgtgcgg cagttcacgt ttgatgtctt cacacgcgcc gataaagtcc
   121141 tgcgcgtaat tgttgtgctc ttcaatgcca gttgcgaccg cgaagatgtt cgggtcgaag
   121201 atgatatctt cagccgggaa gccaacctct tcggtgagga ttttgtacgc ccgacggcaa
   121261 atctcgattt tccgtgcgcg ggtatcggcc tggccctgtt cgtcaaaagc cattaccacg
   121321 actgccgcac cgtagcgacg caacagtttc gcgtgatgga taaaggcgtc gacaccctct
   121381 ttcatcgaga tagagttaac aatgcctttg ccctggatac acttcagacc tttttcaata
   121441 acgtcccatt ttgaggagtc gatcataatc ggcacgcgag cgatatccgg ttcaccggca
   121501 atcagattga gaaaacgcac catcgccgct tcggcgtcga gcatcccttc atccatgttg
   121561 atatcgataa tctgcgcacc gttttccacc tgctgacgcg ccacatccag cgcctcgctg
   121621 tatttctctt ctttgatcag tcgcttgaac ttagcggaac cggtgacgtt ggtgcgttca
   121681 cccacgttca caaacaggct atcttcgcca atgttcagcg gctccaggcc ggacaaacgg
   121741 caggctacgg gaatttccgg tagtttgcgc ggcgctaatc cttctactgc acgactcatc
   121801 gctgcaatat gttgtggcgt ggtgccacag cagccgccga cgatattgag aaagcctgct
   121861 tctgcccatt cacgtatctg ttttgccatc gtgtcggcgt cgagatcgta ttcaccaaag
   121921 gcgttgggca gcccggcgtt cgggtgggcg gtgacgtagc attccgcaat acgcgacagt
   121981 tcctgtacgt actggcgcag ttcgtctggc cccagcgcac agttcaggcc aaaggtcagc
   122041 gcttcggcgt ggcgcaggga gttgtaaaac gcttcagtag tctgcccgga gagcgtgcgc
   122101 ccggaggcgt cggtgatggt gccggagatc ataatcggca gttcaacgcc cagcgcttca
   122161 aactccgttt tcaccgcaaa gaccgccgct ttggcgttaa gggtgtcgaa aacggtttca
   122221 atcaggatca ggtccgcgcc accttccacc agcgctttgg tggattctcg ataagccgcc
   122281 accagctggt caaaagtgat gttgcggaat gccggatcgt tgacgtccgg agaaatagac
   122341 gccgtgcggt tggtcgggcc gagaacaccg gcaacgtagc gcggtttctc cggcgtgcgg
   122401 gcagtccact cgtcggcaca ggcgcgcgcc agtttcgccg ctgcaaagtt gatttccgcc
   122461 gacagggatt ccatctggta atccgccatc gcaatggtcg tggagttgaa ggtgttggtt
   122521 tcgatgatat ccgcacccgc ttcaaagtag gcgttgtgga tagcggcgat cacttccggt
   122581 ttgctgagca ccagcaggtc gttgttgcct ttgagatcgc acggccagtc ggcaaaacgc
   122641 tcgccgcgaa aatcggcctc gttcagtcga taactctgga tcatggtgcc cataccgccg
   122701 tccagcacca gaatacgttc atttaactgc gcacgcagtt gttccacttt gctgctcaca
   122761 cttgctcccg acacgcttaa cccagattta ataaaaattc aacaaaccat actggcataa
   122821 acgcatctgt ggtaaaagcg accaccacgc aacatgagat ttgttcaaca ttaactcatc
   122881 ggatctgttc agcaactatt gcattcgctg ataataaaaa tgaaaatgat ttccacgata
   122941 cagaaaaagg agactgtcat ggtcgcaccc attcccgcaa aacgcggcag aaaacccgcc
   123001 gttgccaccg caccagcgac tggacaggtt cagtctttaa cgcgtggcct gaaattactg
   123061 gagtggattg ccgaatccaa tggcagtgtg gcactcacgg aactggcgca acaagccggg
   123121 ttacccaatt ccacgaccca ccgcctgcta accacgatgc aacagcaggg ctttgtccgt
   123181 caggtcggcg aactgggtca ctgggcgatc ggcgcacacg cctttatggt cggcagcagc
   123241 tttctccaga gccgtaattt gttagcgatt gtccatccta ttctgcgcaa tctgatggaa
   123301 gagtctggcg aaacggtcaa tatggcggtg cttgatcaaa gcgatcacga agcgattatt
   123361 atcgaccagg tacagtgtac tcacctgatg cgaatgtccg cgcctatcgg cggtaaattg
   123421 ccgatgcacg cttccggtgc gggtaaagcc tttctggccc aactgagcga agaacaggtg
   123481 acgaagctgc tgcaccgcaa agggttacat gcctataccc acgcaacgct ggtgtctcct
   123541 gtgcatttaa aagaagatct cgcccaaacg cgcaaacggg gttattcatt tgacgatgag
   123601 gaacatgcac tggggctacg ttgccttgca gcctgtattt tcgatgagca ccgcgaaccg
   123661 tttgccgcaa tttcaatttc cggaccgatt tcacgtatta ccgatgaccg cgtgaccgag
   123721 tttggcgcga tggtgattaa agcggcgaag gaagtgacgc tggcgtacgg tggaatacgc
   123781 tgactttttc gcctctgatt tatacgaatc ggacgctaaa ccgctgcctg cgccgatacg
   123841 cataaacatc ttccacatgc ccttcacgta tgcggttttg tagtgcgcgc cagtaatcag
   123901 cgcggaacaa gtcggcgtgc atctcttcaa atagcggacc aatacgcggg tcggcacata
   123961 accagtgacg gaactcttcc gggaaaacat cgcccggcga gacgctgtac cacggttcgc
   124021 tggcaagttc gtcttccggg tagcgcggca gcgggatgtc gcggaaattc acttccgtca
   124081 tgtagcaaat ttcgtcgtaa tcataaaaca ccacgcgccc gtgacgggtg acgccaaagt
   124141 ttttaaataa catatcccca gggaagatgt tggcagcggc aagctggcga atggcgttgc
   124201 cgtactcttc aatagcgtcg cgcagctgct gaccttccac ttgttccagc cagatattga
   124261 gcggcaccat ccgccgctca atataaagat ggcgaatcac aatctgttcg ccgagatcgg
   124321 tgattttttc cgctgcttcc tggagcagta attccattaa tgccggggaa atatgccgct
   124381 tctccagcac aaagttttca aactcctggg tgtccgccat tcggcccacg cgatcgtgct
   124441 ctttcaccag ttgatagcag gcacgaacgt gagcggcaga catctctttc tgcggcgcga
   124501 acctgtcttt gatgacttta aatacccgat caaaacccgg caacgtaaac accaacatca
   124561 ccattccacg aatacccggc gcttcaatga actgctcatt acagccctgt agataaacga
   124621 gatattcgcg gtagctttcg gttttggcgt gcttctggca gccgatagcc atatacaatt
   124681 cagcggtggt tttacctggc agaatttccc gtagccactc gaccagcgct gcgggcagcg
   124741 gtgcataaac cataaaataa gaacgcgcaa agccaaaaac aatgctcgct tcggcggtcg
   124801 tcgtcaggca ggtatcaata aataactcgc cgtcgtccgt ctggtggatc ggcaataaaa
   124861 atggcaacgt gccggaaggc gtgatcagtt tgcccaccag ccaggctgcc ttattgcggt
   124921 aaaacagttc gttcgccacc tgtaaatgac tttcggcgag gttgtctgtc cccagcgttt
   124981 ccgtcagatg gcgaacgatg taatgaatgt cacggctttt attctgccaa cgcagacgca
   125041 gcggcagatc gctgataacg cgcatcagca gagattccca gccgtgatcg gggtgaaagt
   125101 ctttcgccag tgggcgtgga atggtacgaa agcggcgctc tggctgagag ctaaaaataa
   125161 aaagccgctc gggagtaagc gagcggtggt caaataaccg acagtacacg gagttgaaaa
   125221 agctctccgc aatctcgaag cgcgggtaat ctggcaacag ccgggtgtaa tgccctttaa
   125281 cgcgcagtaa aaatgccgcg tccgtgcttt ggccgttagt aatgcagcgc agttgctcca
   125341 cgaccagacc cacgtgatga tcgtaaagat gaatacggtt tttcatcgcc tgctggacag
   125401 catgccagtc ggcttgctcg aaacgctgct gcgccccgga agtcacttcg aggaatcgac
   125461 catactgagc gtcgaaacct tgcaaaatgg tttgtgcaat cagtaattcc aggccacgcg
   125521 gcatttgttg ccctcgtcgg gagcagagaa ttgccggata agacgcgtaa gcgtcacatc
   125581 aggcgctgat tgccggatgc ggcgtagacg ccttatccgg cctacgttcg gcaacggctg
   125641 taggcatgat aagacgcgca agcgtcgcat caggcgctga ttgccggatg cggcgtgaac
   125701 gccttatccg gcctacagtc agcaacgttt atcggttgct tagaactgcg attcttcggt
   125761 ggagccggtc agcgcggtaa ccgacgacgt gccgccctga ataatggtcg tcactttatc
   125821 gaagtaacct gtacccactt cctgctggtg agatacgaag gtatagccat ctttcgcggc
   125881 ggcaaattcc ggctgctgca ctttctcaac atagtgcttc ataccctcac cctgggcata
   125941 ggcgtttgcc aggtcaaaca tgttgaacca catgctgtgg atacctgcca gggtgatgaa
   126001 ctggaacttg tagcccatat ccgacagctg ctgctggaag ctggcaatag ttttgtcgtc
   126061 gaggtttttc tgccagttga acgaaggcga gcagttataa gccagcagtt tgcccggata
   126121 tttcgcgtgg atggcttgtg caaagcgacg cgccagttcc agatccggcg tggaggtttc
   126181 acaccatacc aggtcagcat atggcgcata cgccaggcca cggctgatcg cttgctcaat
   126241 acccgcatga gtacggaaga agccttcact ggtacgctcg ccagtaataa attcgctgtc
   126301 atatgggtcg caatcggagg taatcagatc cgccgcatcg gcatcggtac gagcaaccag
   126361 cagggttgga acgcccgtca catctgctgc cagacgcgcc gcgaccagtt tctgaatagc
   126421 ttcctgagtt ggcaccaaaa ctttgccgcc catgtgacca catttcttca ctgatgccag
   126481 ctgatcttcg aagtgaactg ccgctgcacc ggcttcaatc atcgctttca tcagttcaaa
   126541 ggcattcagt acaccgccaa aaccggcctc cgcatcggcg acgatcggca ggaagtaatc
   126601 gacatagcgc ggatcgcccg gctcaatgcc cgcggaccat tggatctgat ccgcacggcg
   126661 gaaagtattg ttgatccgct ccaccaccgc tggcaccgag tttgccggat agagcgactg
   126721 atccggatac atgctggccg ccaggttagc gtccgccgct acctgccatc ccgacagata
   126781 gactgcttca atacccgctt tcgcctgttg cagcgcctga ccaccagtca gtgcaccgag
   126841 gctgttgatg tagccttttt tcgactcgcc gtgcagcaga cgccacattt tcgccgcgcc
   126901 cagttgcgcc agggtgcatt ccggattgac tgaaccgcgt aatttcacca catcttccgc
   126961 gctgtacggg cgagtaatgc cttcccaacg cggttgagtc cactctttct gtaattcttc
   127021 aatttgttgt gtacgggttt tcatgtgcag atgctccata gttatgtggt ggtttacgct
   127081 aacaggcggt agcctggcag ggtcaggaaa tcaattaact catcggaagt ggtgatctgt
   127141 tccatcaagc gtgcggcatc gtcaaaacgc ccatgggaga aacgttcttc gcccagttcg
   127201 ctggcaatga ctttcatctc ttcgcccagc atctggcgga acaaggcttt agtcaccggt
   127261 ttgccattgc tcaacgtttt ttgatgatgg atccactgcc agattgaggt acgggaaatt
   127321 tcagccgtcg ccgcatcttc catcaggcca taaatcggca cacagccatt gccagagatc
   127381 cacgcttcga tgtactgcac agccacgcga atgttggcgc gcataccttc ttcggtgcgt
   127441 tcaccatcac aaggtgccag cagctgatcg gcagtaatcg gcgcgtcttg ttcgcgcatc
   127501 acctcaagct ggtttttgcg ggagccgaga atatcgttga ataccgccat tgccgtatca
   127561 gcaaggcccg ggtgagcgat ccatgtgcca tcgtgaccgt tattggcttc cagcgattta
   127621 tccgctttta ctttgtcgag cacctggtta ttgcgctctt catctttgct cggaataaac
   127681 gccgccatgc cgcccatcgc aaaagcaccg cgtttatggc aggttttaat caacaggcgt
   127741 gagtaagcat tcaggaaggg tttatccatc gtcactgcct gtctgtctgg caggacgcga
   127801 tcgggatagt ttttcaatgt tttgatataa ctgaagatgt aatcccagcg accgcagttc
   127861 agaccaacaa tatggtcacg cagcgcgtga aggatttcat ccatctggaa cacggcgggc
   127921 agcgtttcaa tcagcaacgt cgccttgatg gtgccgcgcg gcagattaaa gcgatcttct
   127981 gcatagctga agacttcgct ccaccaggcc gcttcctgcc aggactgggt tttcggcaga
   128041 tagaaatagg gaccgctgcc ctttgccaac agtgcctgat agttgtggaa gaaatagagc
   128101 gcaaaatcaa acaggctacc ggggattgtc tcaccacgcc aggtgacatg tttttccggc
   128161 aagtgcagac cgcgtacccg acaaatcaaa accgctggat tgggcttgag ctgataaatt
   128221 ttgcctgctt cattgctata gctgatggtg ccgttaaccg catcgcgcag gttaatttgc
   128281 ccatcgatca ctttgttcca gtctggtgcc agtgaatctt cgaaatcggc cataaagact
   128341 ttcacattgg cgttgagcgc gttgatcacc atcttgcgct ctacaggtcc ggtaatctct
   128401 acgcgacgat cttctaagtc cgcaggaatc ccgcgaattt tccagtcagc atcacgaatg
   128461 gaagctgttt ccgaaatgaa atcaggcaac gttccgttat cgatatcctg ctgctgctga
   128521 atgcgcgctg ccagaagttt attgcgttgt ggcgtaaaat gcgtcaccag ctcagtcaga
   128581 aattctaccg cttcggcagt aagaatttgc ttctcctgct cgccatacgg ccttgtaaaa
   128641 gccagttcat cgattgttgt tgcctgttca gtcatcgtgc agctcctcgt catggatccg
   128701 aaaacttccc cactgtgaac gaaggatcgt tgtgcgcttt tcgttcagca caactaagac
   128761 tactcattta aaatgcaaaa tcaaaaacaa tttccatttt aattaaaaat acatgttaac
   128821 ttattgataa caatataaat aaaatttatt cacttactga ggtgtgtttc ggaaataaaa
   128881 aaggcacccg aaggtgcctg aggtaaggtg ctgaatcgct taacgatcga ctatcacaga
   128941 agattaatcc agcgttggat tcatgtgccg tagatcatat ggcgtgatct ggtagacgta
   129001 atagttgagc cagttggtaa acagcaaatt accgtgacta cgccagctcg ctcgcggtgt
   129061 attttgcgga tcattgtgcg ggaaatagtt atacggtaca tccgggtcta gcccggcttc
   129121 cacatcgcgg aaatattcct gcgccagcgt ttgcgcatca tattcgggat ggcccgtcac
   129181 aaaggcaatg cgcttatctt tgctggcaaa cagatatgca tccccttctt ccgtctctgc
   129241 cagaatttcc agatcggtat aatcacgaat caacgctgcc ggaaagtcag catagcgcga
   129301 atgcggtgcc aggaatgaat catcaaagcc acgcgtcaga agcgcatgag gatggagaat
   129361 atgatgctcg taaacgccag agagtttgtc ggtgcgggtt tgcttaggaa taccatagag
   129421 gatattgagc gcggcctgta cagcccagca gacaaacagc gtcgaggtga cgtgatcttt
   129481 cgaccactcc agcacctgtt tgatctgcgg ccagtaagcg acatcattaa actccaccag
   129541 gcccagcggc gcgccggtaa ctatcagacc gtcaaagttt tgttcctgaa tatcttcaaa
   129601 gttacagtag aagttattca gatgctctgc gggcgtgttg cgcgattcac gggaatcgat
   129661 gcgcaacagc tgaatatcga cctgcaaagg tgagtttgaa agcaggcgca gaaactgatt
   129721 ttcagtttcg atcttcttcg gcatcaggtt aaggataagc acctttagcg gacgaatttc
   129781 ctgaccagac gcgcgagaag ttgtcatcac aaagacgttt tcttcacgca agaaattgac
   129841 ggcgggtagt tcgtccggca cacgaatcgg cataacctga ttacctcact acatacgctt
   129901 atacgtttag acatccagat agctgaagat aaccagaaaa tttaccaatg tcgagcctgc
   129961 atgttgaagg tgagaaagtt tcaaggaaaa ggctacgtta gaatataaga atgacgaaaa
   130021 ggagagaaaa tggttattag tattcgacgc tcacggcatg aggaggggga ggaactcgtt
   130081 gcgatttggt gtcgttctgt cgatgccact cacgattttt tatcagcaga gtatcgggcc
   130141 gagctggaag agctggtgca ttcctttctg ccggaagcac cgttgtgggt cgcggttaat
   130201 gagcgggatc agccggttgg gtttatgttg ttaagtgggc agcatatgga tgcgctgttt
   130261 atcgatcctg atgtgcgcgg ctgcggcgt
//
LOCUS       DAZFFE010000002       102650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFFE010000002 DAZFFE010000000
VERSION     DAZFFE010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 102650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 102650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..102650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.002"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1348
                     /locus_tag="ACHJ69_000119"
                     /pseudo
     CDS             1..1348
                     /locus_tag="ACHJ69_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
     gene            1413..1661
                     /locus_tag="ACHJ69_000120"
     CDS             1413..1661
                     /locus_tag="ACHJ69_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO5013204.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPGTLSKYAKAGMIFPLPKKVGRH
                     WRVDPRARFVGMVNKPEVIATDHPALKRILEDGAPTKI"
     gene            1639..2769
                     /locus_tag="ACHJ69_000121"
     CDS             1639..2769
                     /locus_tag="ACHJ69_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO5013205.1"
                     /translation="MARPRKYKTEVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKQAEAIAAEANSRLARQQMEQMLTLQEKIIRDTGGSSTVSVFLNSYRKIQQERYENG
                     EIKLNTLKQKASPLRVFDERFGTRPLDAITVKDVVSVLEDYKAKGHNRMGQIFRKVLI
                     DVFREAQQTGDVSPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMKFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGIDTQILLGHSNQKMTDIYNDVRGKEWKKLVI"
     gene            complement(2815..3453)
                     /gene="ompW"
                     /locus_tag="ACHJ69_000122"
     CDS             complement(2815..3453)
                     /gene="ompW"
                     /locus_tag="ACHJ69_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO5013206.1"
                     /translation="MKKLTVAALAVATLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            3691..3780
                     /locus_tag="ACHJ69_000123"
                     /pseudo
     CDS             3691..3780
                     /locus_tag="ACHJ69_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            3808..4551
                     /gene="yciC"
                     /locus_tag="ACHJ69_000124"
     CDS             3808..4551
                     /gene="yciC"
                     /locus_tag="ACHJ69_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO5013207.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            4581..5120
                     /gene="yciB"
                     /locus_tag="ACHJ69_000125"
     CDS             4581..5120
                     /gene="yciB"
                     /locus_tag="ACHJ69_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO5013208.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQSVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            5226..5624
                     /gene="yciA"
                     /locus_tag="ACHJ69_000126"
     CDS             5226..5624
                     /gene="yciA"
                     /locus_tag="ACHJ69_000126"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO5013209.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(5664..6380)
                     /gene="tonB"
                     /locus_tag="ACHJ69_000127"
     CDS             complement(5664..6380)
                     /gene="tonB"
                     /locus_tag="ACHJ69_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO5013210.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQP
                     QYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGK
                     PGSGIVVNILFKINGTTEIQ"
     gene            6604..6900
                     /gene="yciI"
                     /locus_tag="ACHJ69_000128"
     CDS             6604..6900
                     /gene="yciI"
                     /locus_tag="ACHJ69_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO5013211.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            7200..8453
                     /gene="kch"
                     /locus_tag="ACHJ69_000129"
     CDS             7200..8453
                     /gene="kch"
                     /locus_tag="ACHJ69_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO5013212.1"
                     /translation="MSHWTTFKQTATKLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(8508..8681)
                     /gene="yciY"
                     /locus_tag="ACHJ69_000130"
     CDS             complement(8508..8681)
                     /gene="yciY"
                     /locus_tag="ACHJ69_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO5013213.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            8824..10284
                     /gene="cls"
                     /locus_tag="ACHJ69_000131"
     CDS             8824..10284
                     /gene="cls"
                     /locus_tag="ACHJ69_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO5013214.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            10319..10648
                     /gene="yciU"
                     /locus_tag="ACHJ69_000132"
     CDS             10319..10648
                     /gene="yciU"
                     /locus_tag="ACHJ69_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO5013215.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(10701..11705)
                     /gene="oppF"
                     /locus_tag="ACHJ69_000133"
     CDS             complement(10701..11705)
                     /gene="oppF"
                     /locus_tag="ACHJ69_000133"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO5013216.1"
                     /translation="MNAVTEGRKVLLEIAALKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(11702..12715)
                     /gene="oppD"
                     /locus_tag="ACHJ69_000134"
     CDS             complement(11702..12715)
                     /gene="oppD"
                     /locus_tag="ACHJ69_000134"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO5013217.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(12727..13635)
                     /gene="oppC"
                     /locus_tag="ACHJ69_000135"
     CDS             complement(12727..13635)
                     /gene="oppC"
                     /locus_tag="ACHJ69_000135"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO5013218.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(13650..14570)
                     /gene="oppB"
                     /locus_tag="ACHJ69_000136"
     CDS             complement(13650..14570)
                     /gene="oppB"
                     /locus_tag="ACHJ69_000136"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO5013219.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(14656..16287)
                     /gene="oppA"
                     /locus_tag="ACHJ69_000137"
     CDS             complement(14656..16287)
                     /gene="oppA"
                     /locus_tag="ACHJ69_000137"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO5013220.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(17025..17672)
                     /gene="ychE"
                     /locus_tag="ACHJ69_000138"
     CDS             complement(17025..17672)
                     /gene="ychE"
                     /locus_tag="ACHJ69_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO5013221.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(17703..17756)
                     /gene="ychE"
                     /locus_tag="ACHJ69_000139"
     CDS             complement(17703..17756)
                     /gene="ychE"
                     /locus_tag="ACHJ69_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO5013222.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            18149..20824
                     /gene="adhE"
                     /locus_tag="ACHJ69_000140"
                     /gene_synonym="adhC"
     CDS             18149..20824
                     /gene="adhE"
                     /locus_tag="ACHJ69_000140"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO5013223.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDTTGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            20950..21981
                     /locus_tag="ACHJ69_000141"
                     /pseudo
     CDS             20950..21981
                     /locus_tag="ACHJ69_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(22266..22883)
                     /gene="tdk"
                     /locus_tag="ACHJ69_000142"
     CDS             complement(22266..22883)
                     /gene="tdk"
                     /locus_tag="ACHJ69_000142"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO5013224.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            23487..23900
                     /gene="hns"
                     /locus_tag="ACHJ69_000143"
     CDS             23487..23900
                     /gene="hns"
                     /locus_tag="ACHJ69_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO5013225.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(24044..24952)
                     /gene="galU"
                     /locus_tag="ACHJ69_000144"
     CDS             complement(24044..24952)
                     /gene="galU"
                     /locus_tag="ACHJ69_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO5013226.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(25154..26167)
                     /gene="rssB"
                     /locus_tag="ACHJ69_000145"
     CDS             complement(25154..26167)
                     /gene="rssB"
                     /locus_tag="ACHJ69_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO5013227.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(26259..27164)
                     /gene="rssA"
                     /locus_tag="ACHJ69_000146"
     CDS             complement(26259..27164)
                     /gene="rssA"
                     /locus_tag="ACHJ69_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO5013228.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPFVRTNI"
     gene            27277..27735
                     /gene="ychJ"
                     /locus_tag="ACHJ69_000147"
     CDS             27277..27735
                     /gene="ychJ"
                     /locus_tag="ACHJ69_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO5013229.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGATALRAELIAGFAHTEWLGLTVFEHCWQDGGNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            27785..28627
                     /gene="purU"
                     /locus_tag="ACHJ69_000148"
     CDS             27785..28627
                     /gene="purU"
                     /locus_tag="ACHJ69_000148"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO5013230.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            28787..28871
                     /locus_tag="ACHJ69_000149"
     tRNA            28787..28871
                     /locus_tag="ACHJ69_000149"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28821..28823,aa:Tyr,seq:gta)
     gene            28918..29049
                     /locus_tag="ACHJ69_000150"
     ncRNA           28918..29049
                     /ncRNA_class="other"
                     /locus_tag="ACHJ69_000150"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            29303..30502
                     /locus_tag="ACHJ69_000151"
     CDS             29303..30502
                     /locus_tag="ACHJ69_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO5013231.1"
                     /translation="MPARQVCQNFFRGALAPFHKYRQNALLDATIALINGASLTLTSI
                     GRYLPGNAQVKNKIKRVDRLLGNESLHHDIPLIFRNIISMLTSKLSLCVIAVDWSGYP
                     SQEYHVLRASLICDGHSIPLLSWIVPSEKQQNAKIQQAFLNTLSEAVNPKARVIIVTD
                     AGFQNAWFRHIKSLGWDFIGRIRGNKQLHLARKGECWFRRQELQAGNKPEYLGPGTLS
                     RAEYARCDGHFYLHKKEPKGRRNKRSRCGIARPSQIKDARSAAKEPWLIFSSTDDFKP
                     REIMKLYSRRMQIEQNSRDEKSERFGFGLRASYSCSAGRMLVLSLLATLSTIVLWLIG
                     YHAENQGLHLRYQANSIKTRRVISYLRLAENVLRHSPLILKRTVLSTILNHLTRTYQN
                     MVLVYYR"
     gene            complement(30555..31232)
                     /gene="narI"
                     /locus_tag="ACHJ69_000152"
     CDS             complement(30555..31232)
                     /gene="narI"
                     /locus_tag="ACHJ69_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO5013232.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(31232..31942)
                     /gene="narJ"
                     /locus_tag="ACHJ69_000153"
     CDS             complement(31232..31942)
                     /gene="narJ"
                     /locus_tag="ACHJ69_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO5013233.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQIKFFAEKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(31939..33477)
                     /gene="narH"
                     /locus_tag="ACHJ69_000154"
     CDS             complement(31939..33477)
                     /gene="narH"
                     /locus_tag="ACHJ69_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO5013234.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTICSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(33474..37217)
                     /gene="narG"
                     /locus_tag="ACHJ69_000155"
     CDS             complement(33474..37217)
                     /gene="narG"
                     /locus_tag="ACHJ69_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO5013235.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAREAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSSKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(37610..39001)
                     /gene="narK"
                     /locus_tag="ACHJ69_000156"
     CDS             complement(37610..39001)
                     /gene="narK"
                     /locus_tag="ACHJ69_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO5013236.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPLLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            39340..41136
                     /gene="narX"
                     /locus_tag="ACHJ69_000157"
     CDS             39340..41136
                     /gene="narX"
                     /locus_tag="ACHJ69_000157"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO5013237.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALIRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANTSGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADTHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWVQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            41129..41779
                     /gene="narL"
                     /locus_tag="ACHJ69_000158"
     CDS             41129..41779
                     /gene="narL"
                     /locus_tag="ACHJ69_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO5013238.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(41780..43174)
                     /gene="ychO"
                     /locus_tag="ACHJ69_000159"
                     /gene_synonym="iatA"
     CDS             complement(41780..43174)
                     /gene="ychO"
                     /locus_tag="ACHJ69_000159"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO5013239.1"
                     /translation="MSRFIPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     DVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSEEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            43359..43712
                     /gene="ychN"
                     /locus_tag="ACHJ69_000160"
     CDS             43359..43712
                     /gene="ychN"
                     /locus_tag="ACHJ69_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO5013240.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQENNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(43756..44451)
                     /gene="chaC"
                     /locus_tag="ACHJ69_000161"
     CDS             complement(43756..44451)
                     /gene="chaC"
                     /locus_tag="ACHJ69_000161"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO5013241.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAQQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNELLVSVKKLLA
                     ENYPDGVLRPGLA"
     gene            complement(44609..44839)
                     /gene="chaB"
                     /locus_tag="ACHJ69_000162"
     CDS             complement(44609..44839)
                     /gene="chaB"
                     /locus_tag="ACHJ69_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO5013242.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            45109..46209
                     /gene="chaA"
                     /locus_tag="ACHJ69_000163"
     CDS             45109..46209
                     /gene="chaA"
                     /locus_tag="ACHJ69_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO5013243.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            46615..46722
                     /locus_tag="ACHJ69_000164"
     CDS             46615..46722
                     /locus_tag="ACHJ69_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5013244.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(46870..47724)
                     /gene="kdsA"
                     /locus_tag="ACHJ69_000165"
     CDS             complement(46870..47724)
                     /gene="kdsA"
                     /locus_tag="ACHJ69_000165"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO5013245.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(47760..48569)
                     /gene="sirB1"
                     /locus_tag="ACHJ69_000166"
     CDS             complement(47760..48569)
                     /gene="sirB1"
                     /locus_tag="ACHJ69_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO5013246.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGEWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(48573..48965)
                     /gene="sirB2"
                     /locus_tag="ACHJ69_000167"
     CDS             complement(48573..48965)
                     /gene="sirB2"
                     /locus_tag="ACHJ69_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO5013247.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHIQPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(48962..49795)
                     /gene="prmC"
                     /locus_tag="ACHJ69_000168"
     CDS             complement(48962..49795)
                     /gene="prmC"
                     /locus_tag="ACHJ69_000168"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO5013248.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFIFAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(49795..50877)
                     /gene="prfA"
                     /locus_tag="ACHJ69_000169"
     CDS             complement(49795..50877)
                     /gene="prfA"
                     /locus_tag="ACHJ69_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO5013249.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(50919..52175)
                     /gene="hemA"
                     /locus_tag="ACHJ69_000170"
     CDS             complement(50919..52175)
                     /gene="hemA"
                     /locus_tag="ACHJ69_000170"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO5013250.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEERDDLQEALIRWLCDYHNLNEDDLRNSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLFTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQEASEFMA
                     WLRAQSASETIRDYRSQAEQVRDELTAKALAALEQGGDAQTIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            52389..53012
                     /gene="lolB"
                     /locus_tag="ACHJ69_000171"
     CDS             52389..53012
                     /gene="lolB"
                     /locus_tag="ACHJ69_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO5013251.1"
                     /translation="MPLPDFRFIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            53012..53863
                     /gene="ispE"
                     /locus_tag="ACHJ69_000172"
     CDS             53012..53863
                     /gene="ispE"
                     /locus_tag="ACHJ69_000172"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO5013252.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLSTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            54014..54961
                     /gene="prs"
                     /locus_tag="ACHJ69_000173"
     CDS             54014..54961
                     /gene="prs"
                     /locus_tag="ACHJ69_000173"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO5013253.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            55086..56765
                     /gene="dauA"
                     /locus_tag="ACHJ69_000174"
     CDS             55086..56765
                     /gene="dauA"
                     /locus_tag="ACHJ69_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO5013254.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNLEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(56820..57098)
                     /gene="ychH"
                     /locus_tag="ACHJ69_000175"
     CDS             complement(56820..57098)
                     /gene="ychH"
                     /locus_tag="ACHJ69_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO5013255.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            57376..57960
                     /gene="pth"
                     /locus_tag="ACHJ69_000176"
     CDS             57376..57960
                     /gene="pth"
                     /locus_tag="ACHJ69_000176"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO5013256.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            58077..59168
                     /gene="ychF"
                     /locus_tag="ACHJ69_000177"
     CDS             58077..59168
                     /gene="ychF"
                     /locus_tag="ACHJ69_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO5013257.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAVRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(59573..61492)
                     /gene="dhaR"
                     /locus_tag="ACHJ69_000178"
     CDS             complement(59573..61492)
                     /gene="dhaR"
                     /locus_tag="ACHJ69_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO5013258.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSTLGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRG"
     gene            61720..62790
                     /gene="dhaK"
                     /locus_tag="ACHJ69_000179"
     CDS             61720..62790
                     /gene="dhaK"
                     /locus_tag="ACHJ69_000179"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO5013259.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     SEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLAFWDAPVHTPALNWGK"
     gene            62801..63433
                     /gene="dhaL"
                     /locus_tag="ACHJ69_000180"
     CDS             62801..63433
                     /gene="dhaL"
                     /locus_tag="ACHJ69_000180"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO5013260.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQTTQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSCEQNLPVPAALDAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            63444..64862
                     /gene="dhaM"
                     /locus_tag="ACHJ69_000181"
     CDS             63444..64862
                     /gene="dhaM"
                     /locus_tag="ACHJ69_000181"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO5013261.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLSV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAETSGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            65182..66879
                     /gene="treA"
                     /locus_tag="ACHJ69_000182"
     CDS             65182..66879
                     /gene="treA"
                     /locus_tag="ACHJ69_000182"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO5013262.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETSVTPQPPDILL
                     GPLFNDVQNAKLFPDQKIFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPKPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILSRASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPLKQKEAEPTP"
     gene            67216..68238
                     /locus_tag="ACHJ69_000183"
     CDS             67216..68238
                     /locus_tag="ACHJ69_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFO5013263.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASRTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDIQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            68238..69218
                     /locus_tag="ACHJ69_000184"
     CDS             68238..69218
                     /locus_tag="ACHJ69_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO5013264.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            69215..69973
                     /locus_tag="ACHJ69_000185"
     CDS             69215..69973
                     /locus_tag="ACHJ69_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5013265.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTKKLAALYRVSADQIHHHLSAIKH"
     gene            69983..70795
                     /locus_tag="ACHJ69_000186"
     CDS             69983..70795
                     /locus_tag="ACHJ69_000186"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5013266.1"
                     /translation="MLIDDIDFADLYLQQLRLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTSKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVVELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQETLR"
     gene            70792..71646
                     /gene="modD"
                     /locus_tag="ACHJ69_000187"
     CDS             70792..71646
                     /gene="modD"
                     /locus_tag="ACHJ69_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFO5013267.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            71672..73643
                     /locus_tag="ACHJ69_000188"
                     /pseudo
     CDS             71672..73643
                     /locus_tag="ACHJ69_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(73693..73947)
                     /gene="ymgE"
                     /locus_tag="ACHJ69_000189"
     CDS             complement(73693..73947)
                     /gene="ymgE"
                     /locus_tag="ACHJ69_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO5013268.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            74148..74882
                     /gene="ycgR"
                     /locus_tag="ACHJ69_000190"
     CDS             74148..74882
                     /gene="ycgR"
                     /locus_tag="ACHJ69_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO5013269.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLNKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRITAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREARKKADNVRD"
     gene            complement(74884..75495)
                     /gene="emtA"
                     /locus_tag="ACHJ69_000191"
     CDS             complement(74884..75495)
                     /gene="emtA"
                     /locus_tag="ACHJ69_000191"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO5013270.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQIKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            75595..76509
                     /gene="ldcA"
                     /locus_tag="ACHJ69_000192"
     CDS             75595..76509
                     /gene="ldcA"
                     /locus_tag="ACHJ69_000192"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO5013271.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLSVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTQEGTQLTISGHPVLKM"
     gene            76604..78340
                     /gene="cvrA"
                     /locus_tag="ACHJ69_000193"
     CDS             76604..78340
                     /gene="cvrA"
                     /locus_tag="ACHJ69_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO5013272.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIVMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(78397..79467)
                     /gene="dadX"
                     /locus_tag="ACHJ69_000194"
     CDS             complement(78397..79467)
                     /gene="dadX"
                     /locus_tag="ACHJ69_000194"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO5013273.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPYARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHQEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(79477..80775)
                     /gene="dadA"
                     /locus_tag="ACHJ69_000195"
     CDS             complement(79477..80775)
                     /gene="dadA"
                     /locus_tag="ACHJ69_000195"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO5013274.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQTGVKF
                     HFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            81105..82637
                     /gene="ycgB"
                     /locus_tag="ACHJ69_000196"
     CDS             81105..82637
                     /gene="ycgB"
                     /locus_tag="ACHJ69_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO5013275.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKHPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYNGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(82800..83519)
                     /gene="fadR"
                     /locus_tag="ACHJ69_000197"
     CDS             complement(82800..83519)
                     /gene="fadR"
                     /locus_tag="ACHJ69_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO5013276.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            83741..85282
                     /gene="nhaB"
                     /locus_tag="ACHJ69_000198"
     CDS             83741..85282
                     /gene="nhaB"
                     /locus_tag="ACHJ69_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO5013277.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRLQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            85428..85958
                     /gene="dsbB"
                     /locus_tag="ACHJ69_000199"
     CDS             85428..85958
                     /gene="dsbB"
                     /locus_tag="ACHJ69_000199"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO5013278.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMARFPEWLPLDKWVPQVFVASGDCAERQWEFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(86003..87271)
                     /gene="umuC"
                     /locus_tag="ACHJ69_000200"
     CDS             complement(86003..87271)
                     /gene="umuC"
                     /locus_tag="ACHJ69_000200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO5013279.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVHNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADSDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFAFNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPCYTTRSSDLLRVK"
     gene            complement(87271..87690)
                     /gene="umuD"
                     /locus_tag="ACHJ69_000201"
     CDS             complement(87271..87690)
                     /gene="umuD"
                     /locus_tag="ACHJ69_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO5013280.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLTPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            87953..88276
                     /gene="hlyE"
                     /locus_tag="ACHJ69_000202"
                     /pseudo
     CDS             87953..88276
                     /gene="hlyE"
                     /locus_tag="ACHJ69_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            complement(88483..88929)
                     /gene="ycgN"
                     /locus_tag="ACHJ69_000203"
     CDS             complement(88483..88929)
                     /gene="ycgN"
                     /locus_tag="ACHJ69_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO5013281.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKGL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(89021..89680)
                     /gene="ycgM"
                     /locus_tag="ACHJ69_000204"
     CDS             complement(89021..89680)
                     /gene="ycgM"
                     /locus_tag="ACHJ69_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO5013282.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(89752..90045)
                     /locus_tag="ACHJ69_000205"
     CDS             complement(89752..90045)
                     /locus_tag="ACHJ69_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO5013283.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDINK"
     gene            90287..90688
                     /gene="pliG"
                     /locus_tag="ACHJ69_000206"
     CDS             90287..90688
                     /gene="pliG"
                     /locus_tag="ACHJ69_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO5013284.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(90791..91159)
                     /gene="ycgJ"
                     /locus_tag="ACHJ69_000207"
     CDS             complement(90791..91159)
                     /gene="ycgJ"
                     /locus_tag="ACHJ69_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO5013285.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VSSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFSNGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            91679..92374
                     /gene="minC"
                     /locus_tag="ACHJ69_000208"
     CDS             91679..92374
                     /gene="minC"
                     /locus_tag="ACHJ69_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO5013286.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEAPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            92398..93210
                     /gene="minD"
                     /locus_tag="ACHJ69_000209"
     CDS             92398..93210
                     /gene="minD"
                     /locus_tag="ACHJ69_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO5013287.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            93214..93480
                     /gene="minE"
                     /locus_tag="ACHJ69_000210"
     CDS             93214..93480
                     /gene="minE"
                     /locus_tag="ACHJ69_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO5013288.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            93631..93804
                     /locus_tag="ACHJ69_000211"
     CDS             93631..93804
                     /locus_tag="ACHJ69_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013289.1"
                     /translation="MSYLSFEIILIPVKNIIPIITVTLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDN"
     gene            93806..94150
                     /gene="ymgG"
                     /locus_tag="ACHJ69_000212"
     CDS             93806..94150
                     /gene="ymgG"
                     /locus_tag="ACHJ69_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO5013290.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            94154..94489
                     /gene="ymgD"
                     /locus_tag="ACHJ69_000213"
     CDS             94154..94489
                     /gene="ymgD"
                     /locus_tag="ACHJ69_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO5013291.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(94548..96066)
                     /locus_tag="ACHJ69_000214"
                     /pseudo
     CDS             complement(94548..96066)
                     /locus_tag="ACHJ69_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768545.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(96464..96682)
                     /gene="ymgF"
                     /locus_tag="ACHJ69_000215"
     CDS             complement(96464..96682)
                     /gene="ymgF"
                     /locus_tag="ACHJ69_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO5013292.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQH"
     gene            complement(96814..97523)
                     /locus_tag="ACHJ69_000216"
                     /pseudo
     CDS             complement(96814..97523)
                     /locus_tag="ACHJ69_000216"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
     gene            complement(97995..98243)
                     /gene="ymgC"
                     /locus_tag="ACHJ69_000217"
     CDS             complement(97995..98243)
                     /gene="ymgC"
                     /locus_tag="ACHJ69_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO5013293.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIAQALRLRLK
                     DETSVILSDLYLQILQHIEMHKTTLTDIIINDRESVLS"
     gene            complement(98356..98622)
                     /gene="ariR"
                     /locus_tag="ACHJ69_000218"
                     /gene_synonym="ymgB"
     CDS             complement(98356..98622)
                     /gene="ariR"
                     /locus_tag="ACHJ69_000218"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFO5013294.1"
                     /translation="MLEDTTIHNAISDKALSSYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            complement(98651..98923)
                     /gene="ymgA"
                     /locus_tag="ACHJ69_000219"
     CDS             complement(98651..98923)
                     /gene="ymgA"
                     /locus_tag="ACHJ69_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFO5013295.1"
                     /translation="MKTSDNESIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     ENTFRRDLIDSIIRDFSNSDTGGPNRRTAIADNKSMFTGKKINRIH"
     gene            complement(98966..99202)
                     /gene="ycgZ"
                     /locus_tag="ACHJ69_000220"
     CDS             complement(98966..99202)
                     /gene="ycgZ"
                     /locus_tag="ACHJ69_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO5013296.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQENLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            99516..100727
                     /gene="bluF"
                     /locus_tag="ACHJ69_000221"
     CDS             99516..100727
                     /gene="bluF"
                     /locus_tag="ACHJ69_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO5013297.1"
                     /translation="MLTTLIYRSHIRGDEPVKKIEEMVSIANRRNMRSDVTGILLFNG
                     SHFFQLLEGPEEQVQMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSSNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDEEIYTAD
                     LKSKALAFAMAHALKLGDKMISINLLPMTLVNKPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIMKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFSKAKLN
                     GIPSVAWPEKK"
     gene            100932..101663
                     /gene="bluR"
                     /locus_tag="ACHJ69_000222"
     CDS             100932..101663
                     /gene="bluR"
                     /locus_tag="ACHJ69_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO5013298.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTQDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            101884..102288
                     /gene="ycgX"
                     /locus_tag="ACHJ69_000223"
     CDS             101884..102288
                     /gene="ycgX"
                     /locus_tag="ACHJ69_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO5013299.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIRGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            102341..102466
                     /locus_tag="ACHJ69_000224"
     CDS             102341..102466
                     /locus_tag="ACHJ69_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013300.1"
                     /translation="MEIKVQRLSLWMINTVFLLLPINNHQTNTIKLIFEMCSLDF"
     gene            complement(102550..>102650)
                     /locus_tag="ACHJ69_000225"
     CDS             complement(102550..>102650)
                     /locus_tag="ACHJ69_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013301.1"
                     /translation="NAKTVTYDFERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT        26294 a        25859 c        24567 g        25930 t
ORIGIN      
        1 aaatcagagc gcaactgata ctctctcgtc gccagtacca gaaactgatg cagtggaaac
       61 ggcaattccg gacaacgaaa aaaccgaatg caaagtggaa gtcgaaccat ctgtagagcg
      121 tgaggggccg ttctacttcc tcttcaccga caaggatgac gaaaaatacg gtcgcgcaaa
      181 caaactttct ggtctggata aggcgctggc tgccggggct actgaaatca cgaaagaaga
      241 atatttcgcc cgcaaaaaca gtacatactc aggttcacaa caaaatactg gtgcatctga
      301 cacgaccgca caaccagagc cggtaaaagt taccgctgac gaagtaaaca aaattatgca
      361 ggcagccaat atcagccagc ctgacgccga tgaactgctt gcagtatcac gtggtgaatt
      421 tgttgaaggg attagcgccc cgaatgatcc gaaatgggtt aaggggatcc agacccgcga
      481 ttctgtaaac cagaaccagc atgaatcgga acggaactac caaaaagcgg aacaaaacag
      541 cccaaatgcg ttacaaaacg agccagaaac gaaacagcct gaaccagtgg cgcaacagga
      601 agtggaaaaa gtctgcaccg cctgcggtca gaccggcggc ggcaactgcc ctgattgtgg
      661 cgcggtgatg ggcgacgcaa cataccagga aacattcgat gaagagtatc aggtgaagtt
      721 caggaagatg atccggagaa aatggaaggc gctgaacatc cacacaagga gaatgctggc
      781 ggcaatcagc atcacgatag cgataatgaa actggcgaga cggtagatca ctcaattaag
      841 gtgaacggtc atcacgaaat cacatccacc agcaggacgt gtgaccatct aatgataggc
      901 cttgaaacca tgggaaaaaa tcctgatgcc ccgattatct caataggtgc aatatttttc
      961 gatccgcaaa ccggaggtat gggaccggaa tttagtaaga ctatcgatct ggaaactgct
     1021 ggcggagtca ttgatcgtga cgtcattaaa aggtggctga agcaatcacg tgaagcgcag
     1081 tctgccatta tgaccgatga aatcccgtta gatgatgcac tgttacaatt gcgggaattt
     1141 atcgacgaaa actccggtga attttttgtt cagatatggg gaaatggagc caacttcgac
     1201 aacgtaattt tacgccgttc atatgaacgg caggggatcc cctacccgtg gctttactac
     1261 aacgatcgcg ctggacgatg cccgtcacca ggcaaaacaa gtttcagcaa tctggcaaaa
     1321 actgatcccg agtcaggctg atttttaatg ttcaaccccg gtcgtcgtcc gcaagctata
     1381 atggcggcgg ccatgattag cgaacaacgc tcatggcaag acttattctg ctcactgagt
     1441 gggcaaaaga ggaattcagt gaaccggtcc ctactccggg cacgttaagt aaatacgcta
     1501 aagccggaat gatatttcct ctccccaaaa aagttggaag acactggcga gtggatcctc
     1561 gagctcgctt tgtcggaatg gtaaacaagc cggaagtgat cgccacagat caccctgctt
     1621 tgaagaggat actggaagat ggcgcgccca cgaaaatata aaactgaagt tccgggatta
     1681 tctccgtatt ttgacaaaag aaataacaaa gtttactggc gttacaggca tcccataaca
     1741 ggaaaaaatc acgggctcgg cagtattgac cagaaacagg cagaagctat tgcagcagaa
     1801 gcgaacagcc gtcttgccag gcaacaaatg gaacaaatgc tcactctgca ggagaaaatt
     1861 attcgtgata ccggtggttc atcaaccgtt tctgtttttc tgaatagtta ccgaaaaatt
     1921 caacaggaaa gatatgaaaa cggagagatc aaactcaaca cactgaaaca gaaagcatcc
     1981 cctctcaggg tatttgatga acgttttggt accagaccat tagatgccat aaccgtaaaa
     2041 gatgtggtat cggtgctgga agattacaag gcaaaaggac ataacagaat gggacaaatt
     2101 ttcaggaaag tactgattga tgttttccgg gaggcccagc aaacgggcga tgtctcgcca
     2161 ggctttaacc ctgcagaatc ggcaaaaaaa ccacaggtac ggatatcacg gcaacgactg
     2221 acctttgatg agtggatgat gatttataat gcagcggaaa aggatggtta ctttttacag
     2281 cgtggtatgc tgctagcgct gatgacaggc cagcgccttt cagatatttg caaaatgaaa
     2341 ttttcggata tcagggatgg ttatcttcat gtcgaacagc aaaaaacagg aacccggatt
     2401 gccatccctc tggctctgcg ttgcgataaa ttaaatctca ccctggatga tgtagtgtca
     2461 tcctgtcgcg attgcgttct tagtccgtgg ttattgcacc accatcatgc taaagggaca
     2521 gctaagcgcg gcgggatggt taagccagca acgttgacag ttgcatttaa aaaagcccgg
     2581 gattctgtgg attacaactg gcgcgctaat ggcactccac cctctttcca tgagcagaga
     2641 tctttatcag agcgattgtt cagggagcag ggaattgata cccaaatttt gctgggtcat
     2701 tcgaatcaaa aaatgaccga tatttacaac gatgtacgcg gtaaagagtg gaaaaaactg
     2761 gtcatttgat gaccagtttt gcagaggagt tttgcagaga ttttgcagag aaaattaaaa
     2821 gcgatatccg gcagagaaca taaacaccca cggatccagt cgtaccgagt ctttcacggt
     2881 agtaacacca gatttatact tggccgtggt atcgatatcc atgtaccaca ctgacatgtt
     2941 aaccaaccag tcacggttaa tcagataatc aacccccacc tgcccggcag ctccccagga
     3001 gtctttcaga ctgagatcgg aaagccccgc ctctttgcca tgatcgttaa atccattatc
     3061 aaagaaggtg gtgtagttaa tacctgcacc aacgtaagga cggaatttgc tgctggcatc
     3121 accaaaatac cactgcgcca tcagtgttgg cggcagatga tgaacggttg caatatcgcc
     3181 ggtcgcccgg gtgccgattt tatggcggaa cggcgttgct gccagtaatt ccacaccaat
     3241 gttgtcggtc gccatataag taaacgtcag gcccagttga gtgttattgg tcacgctgaa
     3301 tccacccaaa cttcctaacg taccaccagc accttctgtt ggacgtacgg ttgcagaacc
     3361 tgcacgcata aaaaattcgc cggcttcatg cgcaaaagca ctgccagaga gaagagttgc
     3421 tactgccaaa gccgccactg ttaacttttt catatccgct ccgtcgttat ggttataaaa
     3481 acgtgacgaa tatacccaca ttggggtaac aaatgatcct acacagatca cattacatat
     3541 ggtaattcac cctttattga tctggattaa tttagaagtt gcattgaaaa tccaggttaa
     3601 tgtgatttaa atcaattttt atattttcgc tacgcaaata ttaactcgct cgccacttgc
     3661 agcggtttac ccacgcctga aggccagcaa ttacctccgc tttacaaaga tatgcttttc
     3721 catacaagac ctgatgctgc cacccggggc ttaaccaggg tacaattgcc cgctaattaa
     3781 cacctgcata aaactcaagg agagtgcatg tctatcacgg cgcagtccgt ataccgtgac
     3841 accggaaatt tcttccgtaa tcaatttatg accattctgt tggtatcgtt gctatgtgcg
     3901 tttatcacag tggtgttagg gcatgttttc tcacccagtg atgcacagct tgcgcagctc
     3961 aatgacggcg tacccgttag cggcagtagt gggttgttcg acctggttca gaatatgtca
     4021 ccggaacagc aacaaatttt gctgcaggct tcagcggcgt ccactttttc aggattaatc
     4081 ggtaacgcca ttctcgccgg gggcgtaata ttaattatcc agctggtgtc tgcgggtcag
     4141 agagtcagtg cgctgcgagc tattggtgcc agtgcgccga tattgccaaa gttatttatt
     4201 ctgatttttc tgactaccct tttagtacag attggcatca tgctggtggt cgttccgggg
     4261 attatcatgg ccattttact ggctctggct ccagtgatgc tggtacagga caaaatgggc
     4321 gtttttgcct cgatgcgtag cagtatgcgg ctgacatggg cgaatatgcg tctggtggca
     4381 cccgcagtac tgagctggtt gctggcaaaa acactgttgc tgctttttgc ctcttctttt
     4441 gccgcattaa ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa cctcatttca
     4501 gccgtattgc tcatctatct gttccgcctg tatatgttga ttcgccaata accttaagca
     4561 tttgattacg gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg gttgtctttt
     4621 tcgcgtttta caagatttat gacatctatg cggctactgc ggcgctcatc gtcgccacgg
     4681 cgattgtgct tatatatagc tgggttcgct ttcgtaaggt tgagaagatg gccctgatca
     4741 cttttgttct ggtggtcgtc ttcggtggct taacgctgtt cttccacaat gatgagttta
     4801 ttaaatggaa ggttactgtc atttatgccc tgtttgcggg tgccctgtta gtcagccaat
     4861 gggtgatgaa aaagccgcta attcagcgga tgctgggtaa agaactcacc ctgccgcaat
     4921 cggtatggtc gaagctgaat ctggcctggg ctgttttctt tatcctttgc ggtctggcaa
     4981 acatctacat cgcattctgg ctgccgcaaa atatttgggt caactttaaa gtctttggcc
     5041 tgaccgccct taccttaatc ttcacattgt taagcggtat ctatatctac cgccacatgc
     5101 cgcaggaaga taaatcctaa ccagactgcc agtccttcag gggctggcgc tgtatctcct
     5161 ctgttctcct caggaaaatc atagtagcat cgcgcctgtg attttccttt taagtcggtt
     5221 ttaccatgtc tacaacacat aacgtccctc agggcgatct tgttttacgt actttagcca
     5281 tgcccgccga taccaatgcc aatggtgaca tctttggtgg ttggttaatg tcacaaatgg
     5341 atattggcgg cgctattctg gcgaaagaaa ttgcccatgg tcgtgtagtg accgtgcggg
     5401 ttgaaggaat gactttctta cggccggttg cggtcggcga tgtggtgtgc tgctatgcac
     5461 gctgtgtcca gaaagggacg acatcagtca gcattaatat tgaagtgtgg gtgaaaaaag
     5521 tggcgtctga accaatcggg caacgctata aagcaacaga agcattattt aagtatgtcg
     5581 cggttgatcc cgaaggaaaa cctcgcgcct tacccgttga gtaataatca aaagcctccg
     5641 gtcggaggct tttgacttta tgcttactga atttcggtag tgccgttaat tttaaacagg
     5701 atattcacca caatcccact gcctggctta cccggctcat aacgccatct gcgcatcgca
     5761 tttttcacct cacgctcaaa catgtttgca ggcttggctg agaggatttg tacgttatcc
     5821 acgcgaccat ctggcgtgac atcaaattta actttaacct gcccttcaat gcgcaatgcc
     5881 tgtgctcgtg ccggatactg cggctgatta cggcttaatg cgcgtggtcc tgaagccaca
     5941 ctggtaaccg gcttgctggt tgcagccgtt gccgtacttg atgtcgggcg cgcgggtgcc
     6001 gtattttcaa acggtgatgc cggacgcgac tctacgggtt tgacatcgcg ttttggctgc
     6061 tccacctttt tcaccggctt aggttttggc ttaggtttcg gcttcggctt ttcaatgacc
     6121 accggagctt cttttggcgg ttcagggatc ggctcaggtt ccggttctgg ctctaccacc
     6181 ggctccggtg gtggctgaac ggcttgtggc ggttcaagat cagcaggcgc aaccatcgtg
     6241 acagaaatcg gctgcgcggg cgcaggtagt tcaataacct gatgtaccga ggtatagagc
     6301 aaacccgcca caacagcacc atgaatgcag accgaaagta acgtcggcca ggggaagcgg
     6361 cgaggtaaat caagggtcat tgaagtcata atcatttcag tagaaaaacc aggccccgat
     6421 tttaaatgca aatagcaatc atattcaata aggcaagacg atcaggatca gtgaaaatgt
     6481 catttttggc gtaattgacc ttactttatg catggtctta acaaagacat catctttccg
     6541 ttgcagatct tcgccctttc aaataacgta ctttacaact ttcccgaaca aggagttgtg
     6601 cccgtgttgt atgttattta cgctcaagac aaagctgata gcctcgaaaa acgcctttcc
     6661 gttcgtccgg cacatttagc acgtttacag ttactgcatg atgaaggtcg tttgttgacc
     6721 gccggtccaa tgccagcggt agacagtaac gatccagggg ctgcgggatt tactggctca
     6781 accgttattg ctgaatttga gtcgctggaa gcagctcagg cctgggccga tgctgacccg
     6841 tacgtagcgg caggagtcta tgaacacgtt tcagtgaaac catttaagaa agtgttctga
     6901 atagaaattc aatatttaag ggcacggttt ttgcaaccgt gttcttgttt ggcctttcct
     6961 tataggtcgg tcagtgcatt caccaccaca agcagataat aactttatct gtatttcttt
     7021 atatgatgtg aacatattca ggaaaatgcc ctttcctgac gcagagttgc tcactcaaaa
     7081 cctttgatta ctgcggataa agtcatctac tattgtttaa cacattcacg cgctgcatgt
     7141 atgttcagaa aatgtgcacg ttgagataat taaacaaaat tgtggttaca gaggtatcag
     7201 tgagtcactg gactacattc aaacaaacag caacaaagtt atgggtgacc ctacggcacg
     7261 acattctcgc gctggccgtc tttttaaatg gattgcttat ttttaaaaca atctatggta
     7321 tgtcggtcaa tttgcttgat attttccata tcaaagcatt ttcagagctg gatctctcct
     7381 tgctggcaaa cgccccacta tttatgctcg gcgtatttct tgtcctgaac tccattggct
     7441 tactgttccg ggcaaagctc gcatgggcaa tcagtatcat tttgttgttg atagcgctaa
     7501 tttacactct gcatttttat ccctggctga aatttagtat tggattttgc atttttacgc
     7561 tggtgttttt gctgatactg cgcaaagact tctcccacag tagcgcagca gccgggacaa
     7621 ttttcgcatt tattagtttc acgacgttac tgttttactc cacttacggt gcgctttatt
     7681 taagcgaagg ttttaatccg cgaatagaaa gtttgatgac cgcgttctat ttttcgatag
     7741 aaaccatgtc aaccgtcggc tatggcgata ttgtccctgt ttctgaatca gcacgattgt
     7801 tcactatttc ggtcattatt tccggcatta ccgtttttgc cacatctatg acctcaattt
     7861 ttggtccgct tatccgcggg ggattcaaca aacttgtaaa aggaaacaat catacaatgc
     7921 atcgtaaaga tcattttatt gtttgcggac attcgattct cgccatcaat acgattctgc
     7981 aactgaatca acgcggacaa aacgtaacgg ttatcagcaa cttgcctgaa gatgatatca
     8041 agcaacttga gcaacgctta ggcgataacg ccgatgttat ccccggtgac agtaatgaca
     8101 gttcagtgtt aaagaaagcg ggaatcgatc gttgccgggc cattctggcg ctgagtgata
     8161 acgatgcaga taacgcgttt gttgtactct cggcaaaaga tatgagcagt gatgtcaaaa
     8221 cagttctcgc cgtcagtgat agcaaaaacc tgaataagat taagatggta catccggata
     8281 tcattctctc accacaactg tttggcagcg aaattctggc gcgagtttta aatggtgaag
     8341 agattaataa tgatatgctc gtttcaatgt tgttgaactc cggtcatggc attttcagcg
     8401 ataacgatga acaagaaacg aaagctgaca gtaaagaatc ggcgcaaaaa tagagtttaa
     8461 agacggaaga gtaaacttat tgggcaccgc aatggtgccc agtacattta gatattgtag
     8521 atcgcaaaca ataacgagtt acgctgtttg tttagaatgc acttcctgat gctgtggata
     8581 cgcatatttc ggcgcgactg cccctcaagc caacgcgatt tacgtcggct agcctgacgc
     8641 tgcatgcgcc agcgccccac ttccgttcta ctccgcttca tgttaactac tctatgcaac
     8701 aacagaatag tcattatacc cccgcccctg acgctgacca gtgcttttcc cgcgttttta
     8761 tttgccagat gaatccatat gcgtaaactc ataacaatgc gctttcaaaa ggatttctaa
     8821 tctatgacaa ccgtttatac gttggtgagt tggttggcca ttctgggata ctggttgctc
     8881 attgcaggcg taactttacg cattctaatg aaacgacgcg cagttccctc cgcgatggcc
     8941 tggctgttga ttatttacat tctgccgtta gtcggaatta ttgcctatct tgccgttggc
     9001 gagctccatt taggcaaacg ccgcgctgag cgcgccagag cgatgtggcc ttccaccgca
     9061 aaatggctta acgaccttaa agcctgtaag catatcttcg ccgaagaaaa tagcagtgtc
     9121 gctgcgccat tattcaagct ttgcgagcgt cgtcagggga tcgctggggt caaagggaat
     9181 cagctacaac tgatgaccga gtcagatgat gtgatgcagg cgttaatccg cgacatccag
     9241 ctcgcgcgcc ataatattga gatggtgttt tatatctggc agcccggcgg catggcggac
     9301 caggtggctg aatcattaat ggccgctgca cggcgtggta ttcattgccg attgatgctc
     9361 gactccgccg ggagtgtggc ttttttccgc agcccgtggc ccgagctaat gcgtaatgcc
     9421 ggtattgaag ttgtcgaagc cttaaaggtc aatctgatgc gtgtgttttt acgccgtatg
     9481 gacctgcgcc aacatcgcaa gatgatcatg atcgataatt acatcgcgta caccggcagc
     9541 atgaatatgg tcgaccctcg ctacttcaaa caagatgcgg gcgtagggca atggattgat
     9601 ctgatggcgc gtatggaagg ccccatcgcc accgcgatgg ggattattta ttcctgcgac
     9661 tgggagattg aaaccgggaa acgtattctg ccgccgccac cagatgtcaa tattatgccg
     9721 tttgagcagg ccagcggtca caccattcac acaattgctt ctggccccgg ctttccggaa
     9781 gatctcattc accaggcatt attgactgct gcttattcgg ctcgtgaata tttgatcatg
     9841 acaacgccct attttgtgcc aagcgatgat ttacttcatg cgatttgcac ggcggcgcag
     9901 cgcggggtgg atgtcagtat tatccttccg cgaaaaaatg actcgatgct ggtcggctgg
     9961 gccagtcgcg cattctttac agaactgctg gctgctgggg ttaaaattta tcagtttgaa
    10021 ggcgggttac tgcataccaa gagcgtgctg gtcgatggag aactaagtct ggttggcacc
    10081 gttaaccttg atatgcgtag tctgtggcta aatttcgaga ttaccctggc aatcgacgat
    10141 aaaggttttg gtgcagacct cgccgccgtt caggacgatt atatttcgcg ttctcgtctg
    10201 ctcgatgccc gtttatggct aaaacgtcca ttatggcaac gtgtcgccga gcgactgttt
    10261 tacttcttca gcccgttgct gtaaaacgtc gccaacagag gttaaacagg tagtgactat
    10321 ggatatggat ctaaacaatc gcctgactga agatgaaacg cttgagcaag cctacgatat
    10381 ttttctcgaa ctggctgccg acaatctcga tccagctgat gttctgctgt tcaatcttca
    10441 gtttgaagag cgcggcggcg cggagttatt cgatccggcg gaagactggc aggaacatgt
    10501 tgatttcgat ttgaacccgg acttttttgc cgaagtggtg attggtctgg cggacagtga
    10561 agatggtgaa atcaacgatg ttttcgcgcg tattctgtta tgccgcgaaa aagatcacaa
    10621 actttgccat atcatctggc gggaatgaat aatcgctttt ggcgtgaaaa agggctgaca
    10681 actgtcagcc cttattgtgt ttaaagcgga tcgactttca ggcaagaaac ggcgtgtctg
    10741 aaactcccct ccagcacagg acgtgttttg gcacactccg gaccggcaat cggacaacgg
    10801 gtacggaaaa cacaaccgga aggcgggttg atcggcgacg gtaattcccc ttccagtaac
    10861 tggatggttt tgttcttctc cagatccgga tcaggtatgg ggactgccga catcaatgcc
    10921 ctggtgtaag gatgtagtgg attgtggtag acctcatcat aggtccccag ttctaccgca
    10981 tggccgagat acatcactaa cacacgatcg gaaatgtgtt ttaccacggc caggtcatga
    11041 gcgataaaaa ttaatgacaa tcccatctca cgttgcagct gctggagcag gttgaccacc
    11101 tgcgcctgaa ttgacacgtc cagcgccgaa accggctcat cgcagataat cagcttcggt
    11161 tcaagaataa gagcacgagc aatcccgata cgctggcact gcccaccgga gaactcatgc
    11221 ggatagcggt taatcaggtt aggcaataac ccgactttca gcatcatcgc cttcacgcgc
    11281 tcgcgaactt cctggcgtga cattttcgga tgataagtac gcagtggttc agcgatgatc
    11341 tcgccgatgg tcatacgcgg gttcagcgat gccaacggat cctggaaaat catctgaata
    11401 tcactgcgaa cggcacgcca ttcatcgggc ttcatgccca gcaactcttt acctaaccag
    11461 gcaacatgac cgtcggtcgc cttgaccaaa ccgatgatgg cgcgagcaaa ggtggactta
    11521 ccgcatcccg attcccctac cacacctaat gtttcccctt catacaggcg aagagtgaca
    11581 ccatcgacgg ctttgagcgt tttcggcggt tgccagaacc actgtttgcc atctttgatt
    11641 tcaaagtgca ctttcagagc agcgatttca aggaggactt ttcttccttc agttacagca
    11701 ttcataacag ttcctccacc ggtttaaagc aagcacgcag acggccaggc gtaaactctt
    11761 ccagcggcgg tgcgctacta caaatttcca tcgcatgtgg acaacgtggc tggaacgggc
    11821 aacctttcgg taatcgcagc aagtttggcg gattacccgg gatggtcaac attgtttcac
    11881 cttccgcatc gagacgcggc accgcgttga gcaaaccgat agaataagga tgaacgggtt
    11941 gataaaagac atcgcgcgca ttgccatatt ccatcgtgcg cccggcgtac attaccagca
    12001 ctttgtcgca gatccccgcc accacgccaa gatcgtgggt aatcataatg atggcggtat
    12061 taaattcccg cttcagttca ttcaatagcg tcatgatctg cgcctgtacg gtgacgtcca
    12121 gcgcagtcgt gggttcatcc gcaatcagca gcttaggtcg acatagcaat gccatcgcaa
    12181 tcatgactcg ctgacgcatg ccgccagaaa attcgtgcgg gtacattttc atgcgtttac
    12241 gcgcttccgg catttttacc gcatcgagca tccgcaccga ttcttcaaac gcctcagctt
    12301 tgctcatgtt cttatgcagc atcagcactt ccatcaactg ctcaccgacg cgcatatagg
    12361 gattcaacga agtcattggg tcctgaaaaa tcattgagat ttgttcagct cgcagtttat
    12421 tgagttcacg ctctggcaaa ttgaggattt cacgcccatt gaaggttgcc gatccgccaa
    12481 tacgtccgtt ggcagccaac aggcccatca acgcaaatgc ggtttgcgat ttgcccgaac
    12541 cagactcacc tacgatgccc agcgtttctc cggcacgtag cgaaaaattc aaatcattga
    12601 ccgccgtgac gtcgccatcc ggggtactaa aggtgacacg caaatctttc acgttcagca
    12661 gtgcgtcagc ctgttgttgt gcgagcggca cagttgcagt ttcaattacg ctcatggctg
    12721 cactccttaa cgatctttcg ggtcgagggc atcacgcaag ccatcgccga taaagttgaa
    12781 acaaaacagc gttaccacga ggaatcccgc agggaacaac agtaaccatg gagagacttc
    12841 catcgagttc gcgccatcac tcagcaatgc accccagctg cttaacggct cttgcgtacc
    12901 taatcccaag aagctaagga aagattcaaa gaggatcatg ctgggcacca gaagcgacgc
    12961 atagaccacc accacaccga gtacgttcgg cacaatgtgg cgaataacaa tgcccggcgt
    13021 cgatacaccg ccaacttgtg ccgcctcaat aaactctttg cgcttcagac tcagggtttg
    13081 cccacgcaca atacgagcca tatccagcca ggaaaccatg ccaatcgcca cgaaaatcag
    13141 caggatgttt tgaccgaaaa aggtcaccag caaaatgacg aagaacatga atgggaagga
    13201 gttgaggatt tccagcagac gcatcattac cgaatccact ttaccgccca gataaccgga
    13261 aagcgaacca taaagtgtcc ccacgaccac tgccaccagt gccgcagcaa caccgaccat
    13321 gagtgagata cgcccgccaa tcgcaacgcg cacaagcagg tcgcgaccgg atgagtcagt
    13381 accaaagtag tgaccggact ccatatccgg ggcgctggac atcatcgccc agtcagtatc
    13441 gtcataggca aactgcgaaa gcatcggtgc caggattaca aataacgcga tcagcaccag
    13501 cactatcaga ctggcaaccg ccgcacggtt atgcataaaa cgtcgacgtg cgtcctgcca
    13561 caagctgcgc ccttcgacct ccagcttttc actgaaattt tccagcgtct cgctgttttt
    13621 cttacttaac atcatcgcga gctccagtat cagtaacgga ttttcgggtc gataaccgca
    13681 tatagcacat cgacaatggc attaaacaaa atggttaacg cgccaaccag aatggtcaga
    13741 cttaacacta aggaatagtc acggttcaat gcgccattaa cgaacaattg cccaatcccc
    13801 ggtaaaccat aaatggtttc gatgaccatc gaaccggtaa taatgccgac aaatgcgggc
    13861 cccatatagg agagcacggg taacagagca ggttttaatg cgtggcgtaa aatgatccgc
    13921 cgcataggta accctttcgc ccgggcagta cgaataaagt tggagtgtaa aacctcaatc
    13981 atagaaccac gggtaatacg cgcaatactg gcgatatagg cgagtgatag cgccaccatt
    14041 ggcaatatca tgaatttaag cgctccacca ttccagccac cgcccggcag ccaatgcaaa
    14101 atgatcgcaa atatcatgac taataatggc gcaaccacaa aactggggat aacaaccccg
    14161 gtcattgcca gccccatcac ggtatagtcc catttggtgt tttgctttaa tgcggcaata
    14221 acgccagcac taacacccag tattaccgca agaaaaaatg ctgcggctcc cagtttggca
    14281 gaaacgggaa aactggatgc aaccaggtca ttgaccgaat aatctttata tttaaacgat
    14341 ggaccgaaat cgccatgcgc cagttgtttc aggtagctga aatactgtgt catgatcgga
    14401 tcattaagat gatatttcgc ttcgatattg gccatcactt ccggtggtaa agtacgttcg
    14461 ccggtaaaag gactgcccgg agcgaggcgc atcataaaga acgaaatagt aataagaata
    14521 aatagcgtcg gaatcgcttc cagacagcga cgtagaataa attttaacat tgccctacct
    14581 tctggcctgt gccttctgta atgcgataaa aatcagacac cgtggagcag gacactcctg
    14641 ccccacgtat tgccattagt gcttcacaat gtacatattc cgggtgtagg tattatccag
    14701 cggatctttg ccggtatagc caccaaccca cggtttcacc aaacgcgcat tcacgtagta
    14761 ataaacagga acaatggccg aatccttatc cagctgttgt tctgctttag tgtacagagc
    14821 tgtacgctgc gcctcgtcag tcactttcag cgtttccgcc ataatgctgt caaaggccgg
    14881 gctcttataa tgcgcggtat tcattgagct attcgacagc atggtgttca ggaaggaagt
    14941 tggttcgttg tagtctgcac accagcctgc acgggccaca tcaaaagtgc cctggtgacg
    15001 ggtgtcgagg aacgttttcc actcctggtt aaccagtttg acgtttacac caatgttttt
    15061 cttccacaat gaagaggcag caatcgccag ctttttatgc agatcggagg tgttatacaa
    15121 caggttgatg gtcaacggtt tgtctgcggt ataacccgct tcagccagca gttttttcgc
    15181 ttcttcgtta cgtttttcct ggctccagcc aaaccattcc ggctgagtca attttgcgcc
    15241 atcagtatat ggtggagtgt aaccataggc gggcatgtcg ccctgcgctt tcactttatt
    15301 aacaatgata tcgcggtcca tacccaattt cagtgcggta cgcacacgca cgtcattaaa
    15361 tggcggtttc tggttgttaa tttcgtaata gtaagtgcac aggtatggat caacgtgaac
    15421 ttcgtccggg atctcttttt tcagcttctg gaacaattcg atcggcatgt tgttataagt
    15481 catgtcgatt tcaccactac ggtagcggtt gacatcggta acttcagaag caataggcaa
    15541 ataggttacc tggttaataa cggttttcgc gttgttccag taggtcgggc tgcgttcaag
    15601 aacgattcgt tcgtttacga cccaatcttt taaggtatag gcaccgttgg tgacgatatt
    15661 accaggctgg gtccattttt cgccgaattt ctcgatagcg gcttttggca ccggtgaagt
    15721 tgatgggtga acaagtaatt tatagaagta cggaacaggt tcacttaagg tgacttctaa
    15781 tgtgtgatca tcaatagctt tcacgccgag atcggtaatc ggttttttcc cttcaagaat
    15841 ttcatcaata ccggcgatat gcccatattg cagataactg gcatacggag aagcagtgtt
    15901 tggatcaaca gaacgttgcc agctatacac aaagtcttgt gctgtaactg gcgtgccatc
    15961 agaccatttc gcatctttac gcaaatggaa ggtccagact ttcgcgtctt tattatccca
    16021 tgattcagcg acgccaggtg ccggatgacc gtcaagatcg ctgaccagta agccttcaaa
    16081 caggtctcgg ctgatattag actccggaac accttcaatt ttgtgcggat ctaatgactg
    16141 aacttctgaa ccattgttac gtaccagcgt ttgtttttcc gccagtgtga cacctgcggg
    16201 tacatcagct gccagcgcga cattccctgc cataagcgca gccaaaacgc cagctgctac
    16261 taaacttctc ttggtgatgt tggtcattgt tttttggact ccctcattat aattactggt
    16321 attaaccagc atgtgtaatc ccggattggg accctgcaca ggctcggaga ctttctgctg
    16381 tcaggttact ttatcgttac gtattactat caccgacttt atttattctg gtgattcaga
    16441 cgatcacctt atgtcgattc gtactgtgtc tcccctgtcg agacacatta ttctttcgcg
    16501 acgatcttta attgttaatt attctcatta ccaataaaga atcgcctggc ggttgcccgg
    16561 aaagtaccaa atgcggttct tactcgccaa tacattttgc aaaattgtta agcaattctc
    16621 ttttattgac cgtatcagaa tctatagctt gcatccatgc cttaacaaac atctttcaat
    16681 tcaatcaaat agaggttggt taagcatgtc tctcttttct gattacagtt ccagtagtga
    16741 aatgcacaat aatttaacaa ttgattatta tttagcactt tcctctacca aaggtagtgg
    16801 aattactaat atcatttcaa ttattcttca gcaagcccag gattatgatg tggcgaaaat
    16861 aacatgatgg tgaggagaag ccgttaagga aatccctata actaactgta atttgagaag
    16921 aatattttta cctattcctt ttgcttttga taactgcgca ggaacaaagt aaaaataatg
    16981 acgaagtaaa cggagcatgg cagctccgtt tcatttgaaa ggaattaatt aagcaggccg
    17041 gggaaaatcc ccttaatacc agtaacgata aattcaatcc ccaacgccat cagcaatagc
    17101 cccataatac gcgtaatcac gttgatgccg gtctggcgta aaactcgtac cagccacggt
    17161 gccatgcgga acaatcccca gcaacataaa gcgaacaatg caatagccac aaagaaacca
    17221 aacagatagc taatgctgtg ataacgcgta ccccagacga tggtagaact gatcgcccct
    17281 ggccccgcca tcaacggcaa cgccaatggc accacaccaa tgctttcacg taccgcggtt
    17341 tctgattttt cttgcttgtt ctgtttatcc tcaccaagct tgccgctgat catcgacatc
    17401 gctattgtca ccaccaggat gccaccggcg atacggaatg aatcaattga tataccaaaa
    17461 agttgtagaa tcgtgtcgcc gagaaaaagc gagatccaca agataatggc cacagacagg
    17521 ttggctgtaa ggttagtttt gtttcgcgct gccgctgtct gataactggt catgctgata
    17581 aagacgggaa taatccctac cgggttgacc agcgcaaata acccgatgaa aaatttgaag
    17641 taaacgggaa aatcaaaaag ggtctgaatc acggttagct ccgaagcaaa agccggataa
    17701 tgttagccat aaataaggtt gaaaagacgc gctgacaata cgccttttga cagcattttt
    17761 cacctcctaa ctacttaaaa ttgctatcat tcgttattgt tatctagttg tgcaaaacat
    17821 gctaatgtag ccaacaaatc atactacaat ttattaactg ttagctataa tggcgaaaag
    17881 ccatgctgaa aggtgtcagc tttgcaaaat tttgatttgg atcacgtaat cagtacccag
    17941 aagtgagtaa tcttgcttac gccacctgga agtgacgcat tagagataat aactctaatg
    18001 tttaaactct tttagtaaat cacagtgagt gtgagcgcga gtaagctttt gatttccatc
    18061 ggttaagcaa atcatcaccg cactgactat actctcgtat tcgagcagat gatttactaa
    18121 aaaagtttaa cattatcagg agagcattat ggctgttact aatgtcgctg aacttaacgc
    18181 actcgtagag cgtgtaaaaa aagcccagcg tgaatatgcc agtttcactc aagagcaagt
    18241 agacaaaatc ttccgcgccg ccgctctggc tgctgcagat gctcgaatcc cactcgcgaa
    18301 aatggccgtt gccgaatccg gcatgggtat cgtcgaagat aaagtgatca aaaaccactt
    18361 tgcttctgaa tatatctaca acgcttataa agatgaaaaa acctgtggtg ttctgtctga
    18421 agacgacact tttggtacca tcactatcgc tgaaccaatc ggtattattt gcggtatcgt
    18481 tccgaccact aacccgactt caactgctat cttcaaatcg ctgatcagcc tgaagacccg
    18541 taacgccatt atcttctccc cgcacccgcg tgcaaaagat gcaaccaaca aagcggctga
    18601 tatcgttcta caggctgcta tcgctgccgg tgctccgaaa gatctgatcg gctggatcga
    18661 tcaaccttct gttgagctgt ctaacgcact gatgcaccac ccagacatca acctgatcct
    18721 cgcgactggt ggtccgggca tggttaaagc cgcatacagc tccggtaaac cagctatcgg
    18781 cgtaggcgcg ggcaacactc cggttgttat cgatgaaact gctgatatca aacgtgcagt
    18841 tgcatctgta ctgatgtcca aaaccttcga caacggtgta atctgtgctt ctgaacagtc
    18901 tgttgttgtt gttgactctg tttatgacgc agtacgtgaa cgtttcgcaa cccacggcgg
    18961 ctatctgttg cagggtaaag agctgaaagc tgttcaggac gttatcctga aaaacggtgc
    19021 gctgaacgcg gctatcgttg gtcagccagc ctataaaatt gctgaactgg caggcttctc
    19081 tgtaccagaa aacaccaaga ttctgatcgg tgaagtgacc gttgttgatg aaagcgaacc
    19141 gttcgcacat gaaaaactgt ccccgactct ggcaatgtac cgtgctaaag atttcgaaga
    19201 cgcggtagaa aaagcagaga aactggttgc tatgggcggt atcggtcata cctcttgcct
    19261 gtacactgac caggataacc aaccggctcg cgtttcttac ttcggtcaga aaatgaaaac
    19321 ggctcgtatc ctgattaaca ccccggcttc tcagggtggt atcggtgacc tgtataactt
    19381 caaactcgca ccttccctga ctctgggttg tggttcctgg ggtggtaact ccatctctga
    19441 aaacgttggt ccgaaacacc tgatcaacaa gaaaaccgtt gctaagcgag ctgaaaacat
    19501 gttgtggcac aaacttccga aatctatcta cttccgccgt ggctccctgc caatcgcgct
    19561 ggatgaagtg attactgatg gccacaaacg tgcgctcatc gtgactgacc gcttcctgtt
    19621 caacaatggc tatgctgatc agatcacttc cgtattgaaa gcagcaggcg ttgaaactga
    19681 agtcttcttc gaagtagaag ctgacccgac cctgagcatc gttcgtaaag gtgcagaact
    19741 ggcaaactcc ttcaaaccag acgtgattat cgcgctgggt ggtggttccc cgatggacgc
    19801 tgcgaagatc atgtgggtta tgtacgaaca tccggaaact cacttcgaag aactggcgct
    19861 gcgctttatg gatatccgta aacgtatcta caagttcccg aaaatgggtg tgaaagcgaa
    19921 aatgatcgct gtcaccacca cttctggtac aggttctgaa gtcactccgt ttgcggttgt
    19981 aactgacgac actactggtc agaaatatcc gctggcagac tatgcactga ccccggatat
    20041 ggcgattgtc gacgccaacc tggttatgga catgccgaag tccctgtgtg ctttcggtgg
    20101 tctggacgca gtaactcacg ccatggaagc ttatgtttct gtactggcat ctgagttctc
    20161 tgacggtcag gctctgcagg cactgaaact gctgaaagaa tatctgccag cgtcctacca
    20221 cgaagggtct aaaaatccgg tagcgcgtga acgtgttcac agtgcagcga ctatcgcggg
    20281 tatcgcgttt gcgaacgcct tcctgggtgt atgtcactca atggcgcaca aactgggttc
    20341 ccagttccat attccgcacg gtctggcaaa cgccctgctg atttgtaacg ttattcgcta
    20401 caacgcgaat gacaacccga ccaagcagac tgcattcagc cagtatgatc gtccgcaggc
    20461 tcgccgtcgt tatgctgaaa ttgctgacca cctgggtctg agcgcaccgg gcgaccgtac
    20521 tgcagctaag atcgagaaac tgctggcatg gctggaaacg ctgaaagctg aactgggtat
    20581 tccgaaatct atccgtgaag ctggcgttca ggaagcagac ttcctggcga acgtggataa
    20641 actgtctgaa gatgcattcg atgaccagtg caccggcgct aacccgcgtt acccgctgat
    20701 ctccgagctg aaacagattc tgctggatac ctactacggt cgtgattatg tagaaggcga
    20761 aactgcagcg aagaaagaag ctgctccggc taaagctgag aaaaaagcga aaaaatccgc
    20821 ttaatcagta gcgctgtctg gcaacataaa cggccccaga aggggtaatg ccagtcagtt
    20881 aaggatcagt tgaccgatcc agtggctgtg taagaatccg gaaacgctca ctcgtttccg
    20941 gattttttta tgctcattgg acaggccctt gatttggtat cccgttacga ttctctgcgt
    21001 aacccactga cttctctggg ggattacctc gcccccgaac tcatctctcg ttgccttgcc
    21061 gaatcaggta ctgtaacgct acacaagcgc cgtcttcccc tcgaaatgat ggtctggtgt
    21121 attgttggca tggcgcttga gcgtaaagaa cctcttcacc agattgcgaa tcgcctggac
    21181 atcatgctgc cgggcaatca ccccttcgtt gcccccagtg ccgttattca gacccgccag
    21241 cgtctgggaa ttgaggctgt ccgccgcgtg ttcacgaaaa cagcgcagct ctggcataac
    21301 gccacgccgc atctgcactg gtgcggcctg accctgctgg ccatcgatgg tgtgttctgg
    21361 cgcacaccgg atacaccaga gaacgatgca gccttccccc gccagacaca tgccgggaac
    21421 tcggcgctct acccgcaggt caaaatggtc tgccagatgg aactgaccag ccatctgctg
    21481 acggctgcaa gaacagcgaa aatgagcttg ctgagcaact tatagaacaa accggcgata
    21541 acactctgac gttaatggat aaaggttatt actcaccagg actgttaaat gcctggagcc
    21601 tggcgggaga acaccgccac tggatgatcc ctctcagaaa gggagcgcaa tatgaaaaga
    21661 ttagaaaact gggtataggc gatcatctgg tgaagctgaa aaccagcccg caggcacgaa
    21721 aaaagtggcc gggactggga aatgaggtga cagcccgcct gctgaccgtg acgcgcaaag
    21781 gaaaagtctg ccatctgctg acgtcgatga cggacgccat acgcttcccc gggacgtata
    21841 ccggagctga tgcgcgatct tgcaagtatg ggacaaccag tgaaattacc gacaagaagg
    21901 gaaagggcct tcccgagagt ggtaaaggag aggccctgga aataccccac agccccgaaa
    21961 aagagccagt cagttgctta actgactggc attacccctt ctggggccgt tttttgtttt
    22021 cccaaaacaa cctttccata aaccggcagc attgtccttc atcatatttg cgacgatgta
    22081 taacgcctac acacagcgat attgtacttt acaggttaca agtcaatgtc ggtgtataag
    22141 cgctctgtga ggcgtacagc ggcgttaaaa aactgccgag aagggtgtat agcccggaag
    22201 aagtgcgtaa aacgaactga caggataaaa gcgccctgct caccctgtca gtaaagaaat
    22261 tcttattaat cgtggcgatg cctttcctga atagccgtta atgagccgac ttctaacgct
    22321 tctttatagt gtttacggca tacagaaacg tagcgttcat taccaccaat aaccacctgc
    22381 tcaccttcgt tataaggtct gcctgcttga tcaagacgca gcaccatgct tgctttacgg
    22441 ccacaaaaac agatggtttt taattcaacc agtttgtcgg accatgccag taagtattga
    22501 ctgccaataa ataattcacc tcgaaaatcg gtacgtagac cataacaaag tacgggtata
    22561 tcgagttgat cgacaacctc cgataattca tatacttgtt gtctggttaa aaactggcat
    22621 tcatcaacca gtacgcaatg aattgcctgc tgttcatgtt ccgcacgaat ctcatcaaat
    22681 aatgatgaat tttggttaaa taattttgca ggtgatgaca aacctatacg cgaactgact
    22741 ttcccggcac caaagcgatc atcaatttct gccgtatata cgacagtgcg catgccgcgt
    22801 tcctggtaat tgtatgaaga ttgcaacaat gctgtagact tacccgcatt cattgcggaa
    22861 tagtagaaat atagctgtgc catcagccac aggccctcaa tgatatgaaa taaagttggc
    22921 tggagtttat cataattcgt cagtttttca gctcaacagt atgcacagaa gtggtatatg
    22981 cgttctccct tacgaagcct tacataatcc ttctgagcta tcattacaac tgcctcgctt
    23041 gttataagcg ggtaaatgac tgccggtaac tattcacaat ctttaacctg ttgcgcatgt
    23101 aatagccctc tgttgacctc caggagatag tgcaatacta agtccctgct cttattgcga
    23161 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacgac taatacagaa
    23221 gactgaaagg tcgtcagcct acgattatct ccccataaaa tgtgacatga atcaggaagt
    23281 tttaacctca cgtgctgcga aatcatcggt gcaaataggg ctatatgccg cgtcttttct
    23341 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
    23401 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
    23461 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
    23521 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
    23581 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
    23641 aagagcgcac tcgtaaactg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
    23701 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aagcgtgctc
    23761 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
    23821 agggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
    23881 atttcctgat caagcaataa tcttttgtag attgcacatg ctaaaaatcc cgccatcggc
    23941 gggatttttt attgtccggt ttaagacaat ataataagaa taagctataa aaaaacggcg
    24001 tcgattgctc aacaccgttt cgtggaaaac accgatacgg atgttacttc ttaatgccca
    24061 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
    24121 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
    24181 gataggcttc aaccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
    24241 catcaccagc tccaggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
    24301 aacgacccac aatagcgaga ttagacggcg caacatccgc ttttggtttt tcaaccacgc
    24361 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
    24421 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
    24481 ggcggatcat ctctgccagg ttatcctgtg ataaatcgga ttcatattca tccagaataa
    24541 cgtcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
    24601 cgtgtcccag gcctttcgcc agcccctgac gaacttgcat aatagtcacg tgcggcggac
    24661 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
    24721 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtga gtaaccagca
    24781 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
    24841 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
    24901 atcccgctac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
    24961 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
    25021 caccccgaag gggctaagtt ctgaaaagga ggcgagttag cataattaag acaattactt
    25081 atacgaatag cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
    25141 tgttatcgtt tgctcattct gcagacaaca tcaagcgcag ccgaccaccg gttccccata
    25201 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
    25261 tgcctaacgg aacaccatta ctgatttgca cctgatgttc gccggtattt aacgtcgcat
    25321 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
    25381 gcggaaactg ccccggcaga ttggcctgac ggagtaaatg gtttacctgc ttcaataacg
    25441 cgcccaactc tggcaaccgt tgattttgat gtgcaagctg ttcctgtaat aatccgttaa
    25501 acaatgcgcg taataacaag gcagcaagta cgccattatg cccagctctg gtgacatcaa
    25561 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
    25621 tgtccgcggc aactaattga cgataattaa cccggcaatg ggaaatcacc tgctgaactg
    25681 gcggttgtag ttcctgtaat aatttcgccg ctgcagcagg gttatcaacc attgcatccc
    25741 agtcgcgaaa aagcctttcc tcttcctcaa cgcgggaatt aaacatgctg ggatagagac
    25801 aggcaaaaac catctcgcgc aagcgattca gatctttaac cggtttcagc aaaacatctt
    25861 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
    25921 gaactggtgt ctggtcacct ctattgcgta tatgttccag cagtttaagc ccgttcattc
    25981 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
    26041 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac cacgaatcca
    26101 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
    26161 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcacgctatt gccacctacg
    26221 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtacaaac
    26281 ggcaaaagtt cgtccatttt cttttccact gccagctgcc cggccgcaat ggcagcgtgc
    26341 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
    26401 ggatcacctg ccatgcggtt ccttttaagg cggttctcca gcacctggat agaagtggtc
    26461 ataatctctg ttgccgttgg cgctgtcacc gcccgacgtg tcgttatgct gcctaacctt
    26521 tctttcagac gcgcatgcca cggcagagaa tcaccattct cgctattttc ttcactgaca
    26581 ttaaaggaga gcaaatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
    26641 acaatatcag cccccaatgc acgcgtgagg gaaataggaa ttgggttaac tacagcgcca
    26701 tcaaccagcc agtagccgtt atgcgcaacg ggtgccatta gtcctggaat actgcatgat
    26761 gcacgaatag caagatggag atcgccttca gtaaaccata attcacgccc agtacttaaa
    26821 ttggtggcaa cagccgcgaa gcgacgggaa caattttcga tctctgtttc cggcattatt
    26881 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagaga
    26941 tccatcaggc gtaaaacatc ccaataactg aaagaggtca cccaatcttc cagcgcagat
    27001 aatcgatcgc atgcataggc agcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
    27061 tcaatttcaa tacccacttt ttttagcgca ttaattacgc caatatgcga ccaacctctt
    27121 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
    27181 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
    27241 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
    27301 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
    27361 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgcagattat
    27421 ttaataaaga cctggcatcc atcttgtgga gcaacggcgt tacgtgccga attgattgcc
    27481 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgctg gcaggacggc
    27541 ggcaatatcg gatttgtcag ttttgttgct cgatttaccg agggtggcaa aacaggtgca
    27601 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
    27661 cgtccgcagt ttggccgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
    27721 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
    27781 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
    27841 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtgc agaacaatga
    27901 atttgtagat catcgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
    27961 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
    28021 gaatcctgcc ggtcgtcgcc ggatagtaat tctggtcact aaagaagcgc attgccttgg
    28081 cgatttgttg atgaaagcca attacggtgg cctggatgtc gaaatcgcgg cagttattgg
    28141 taaccacgat actttacgtt ctctggttga gcggtttgat attccgtttg agctggtaag
    28201 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggatgcca ttgatgctta
    28261 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
    28321 acgcttcccg aataagatca tcaatattca ccattcattc ctgccagcgt ttatcggcgc
    28381 acgtccttat caccaggcct atgagcgtgg cgtgaagatt attggcgcaa ccgctcacta
    28441 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
    28501 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
    28561 tcgtgcgcta tacaaagtac tggcgcagcg cgtctttgtt tacggtaatc ggacgattat
    28621 tctttaatcg ccagcaaaaa taactggtta gctttaatcc gttacggatg aaaattacgc
    28681 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
    28741 cccgcttccc gataagggag caggccagta aaaagcatta ccccgtggtg gggttcccga
    28801 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
    28861 ccccaccacc atcactttca aaagcccctg aattctcaaa cgaatccgca attaaatatt
    28921 ctgcccatgc ggggaaggat gagaggcttc gaccaaggtt cgactcgagt gccagcgaga
    28981 gagcgttgcc gcaggcaacg acccgtaggg cgaagcgcgc agcgctgagt aatccttccc
    29041 ccaccaccat ttcttcctga aatactcaaa acctgttact ccgagctaat tctgcccaat
    29101 ggcaccgcgt ttaccggacc taaaatatcc tcccctgtag gtcggataag cgaagcgcat
    29161 ccgacaactc cccccgcacc gtgcccataa aaaaaacccg ccggagcgct gaggaatccc
    29221 cagtaatggg cgggtaaaaa aagacaggca tagagttttg tgatctactg acttgtgcaa
    29281 ccaattcaat gagatctcct ctatgcctgc tcgccaagta tgtcagaatt ttttccgcgg
    29341 tgctttagca ccatttcaca aataccgaca aaatgctttg ttggatgcca ctatcgcact
    29401 aatcaatggc gcatctctga cgctgaccag catcggacgt tatttacccg gtaatgctca
    29461 ggtcaaaaat aaaataaaac gtgttgatcg tctgctcggt aatgagtctc ttcatcacga
    29521 catccccctg attttcagaa acattatttc catgctgacg agcaagctgt cactgtgtgt
    29581 catcgccgtt gactggagtg gatacccctc tcaggagtat cacgtactcc gcgccagcct
    29641 gatttgcgat ggacattcga ttccattgct gagttggata gttccttcgg aaaaacagca
    29701 aaatgcaaag atacagcaag cctttcttaa tactctttcc gaagcggtaa acccaaaagc
    29761 aagggtaatc atcgtcacgg atgcagggtt ccagaatgcc tggtttcggc acataaaatc
    29821 actcggctgg gattttattg gccggatacg aggcaataaa caactgcatc tggcgagaaa
    29881 aggggaatgc tggttcaggc gtcaggaatt acaggccggt aacaaacctg aatatctggg
    29941 gccggggacg ctttcccggg cggaatacgc ccgttgcgat ggtcattttt atttgcacaa
    30001 aaaagagccg aaaggccgga gaaataaacg ttcgcgttgc ggtatagcca gaccatcaca
    30061 gataaaagat gccaggtcgg cggcaaaaga accatggtta atattcagca gcacagatga
    30121 ctttaaaccg cgagaaatca tgaagttata cagccgccga atgcagattg agcaaaactc
    30181 cagggatgaa aaaagtgagc gttttgggtt cggtctgcga gccagttaca gctgttcggc
    30241 aggaagaatg ctggtactga gcctgctggc aacactgagc acaatagtgc tgtggcttat
    30301 tggctatcac gctgaaaatc aagggttaca tcttagatat caggccaaca gcattaaaac
    30361 gcggcgggta atctcgtatc tgaggttagc ggagaatgtc ttacgacact ccccgctaat
    30421 tttaaaacga acggtgctaa gcaccattct taatcacctc accagaacct accagaatat
    30481 ggtgttggtt tattatcgct gatttatggg gatccctcag cgccgaagca gggtctatgt
    30541 gaactaaaat tcgcttagtg acgagcgcgc accagctgat acttacgcgt cagatactcc
    30601 accgggacgc tccagatgtg taccagacgc gagaacggga acagcaggaa taacgtcatc
    30661 cccaacacca ggtgcagacg gaagataaac gccacgccat caagatgttg agaagcgcca
    30721 ccgtggaagg tcactacaga ctgcgcccag ccaaccagtt tcatcatctc gctaccgtcc
    30781 atatgctggg cggagaacgg aatggtcagc aggcccagcg cgcactggat aacgagcagc
    30841 gacaggatca ggatatccgc tccggtagtc gttgcacgca cgcgtgggct gaacagacga
    30901 cgtttcaaca acagcacgcc gccaatcaga cacagtacgc cgctggcacc accagcaaac
    30961 atcgccattt tctgtttcac ttcaatcggc agccaggctt catacatcca gtgcggcgtc
    31021 agcataccga agaagtgacc gacaaaaatc cccagaatac cgatatggaa caggttcgac
    31081 gccaggttca tccctttgcg atccagcatc tggctggacg ccgcacgcca tgtgtactgc
    31141 ccgtagtcat aacgcagcca gctaccaatc aggaagaccg ccccggctat gtacgggtag
    31201 atatcaaaga agaacatatt caggaattgc attagtgctg tcctccggtg gtgatattca
    31261 gatattgcgg cgcgacggca ccggcaaagc gacgctgatg agcagtgatt gctgaatcac
    31321 cgcagccttt ttcagcaaag aatttaatct gctcttcttc ccagactgca tccagcgcct
    31381 gcggcgtatc gtcgcgcgct tcatcggcaa ttttttccgc cactttgtcg ctgtcgattg
    31441 cggtattcgc cagtttcagc agcagatcaa acagcacggc ataacggctt tcacgctgtt
    31501 gcagacgcgc gctcagcaat gccagaatcg gcgcaatatc tttcaaaccc tccacggctt
    31561 cgctttgcgg cagctgcgcc aggtactcca gatacagcgg cagatggtcg ggcaattcgc
    31621 ggctgtttaa ctgcaagccg tgctgctcgt actgcgccag cagatccacc atcgcctggc
    31681 cgcggtcacg ggattcaccg tgaacatgtt caaatagcaa cagtgacgtg gcacggccac
    31741 ggtcgaacag ttcgctgtac tgcgcctggg catcgagcgg atccatcgtc gttaaatcgc
    31801 gcaggaaaat gcccagcgca tgggcatcct cttttggcag atttttcgac gcggcaatcg
    31861 cctcaaacat ctcttgttga tgctgccata aggcagcatc cggatattca aggagacgcg
    31921 atacaatcac gagttcgatc atgggtgcgg ctccgttttg ctggttacgt cgatggcatc
    31981 gatacggcgg ctgttgaaca gattgaattt ggtatctgaa ccgtggcagc catcaccaaa
    32041 ggtaaagccg cagccatttt tctccgggaa ggcttcccgc gccagttcac gatgactgct
    32101 cggcaccaca aagcgatctt cgtagttggc aatagccaga taacgataca tctcctgtgc
    32161 ctgggcttcg gtcagaccga cctcttccag cgcacgggta tcgactttac cgtcaacggt
    32221 ttcagcacgt ttgtaatgac gcatcgccag catacgtttc agcgcgcgca gtaccggttt
    32281 ggtatcaccg gcggtcagca gattcgccag atactgtacc ggaatacgca ggctttcgac
    32341 gtctggcaga atgccgttgc tgcccagttc acccgcgtct gccgcagact gaatcggcga
    32401 cagaggcggc acgtaccaga ccatcggcag tgtgcgatat tccggatgca gcggcagcgc
    32461 cagtttccat tccatcgcca ttttataaac gggcgactgc tgcgcagcct caataacgct
    32521 cagcggaata ccgtctttaa tcgcctgctc gatgactttc ggatcgttcg gatcgaggaa
    32581 cacatccagc tgacgctggt agagatcttt ctcgttctcg gtacttgccg cacgttcaat
    32641 cgcgtcggca tcgtacagca gcacgccaag ataacggata cgaccgacac aggtttctga
    32701 acatattgtc ggctggccgg cttcaatacg cggatagcag aagatgcact tctcagactt
    32761 accgctcttc cagttgaagt agattttttt gtacgggcat ccggtgatgc acatacgcca
    32821 gccacggcat ttatcctggt cgatcaggac gatgccgtct tcttcacgct tgtaaatcgc
    32881 accgctcggg caggtcgcca cacatgccgg gttcagacag tgttcgcaca ggcgcggcaa
    32941 atacatcatg aaggtgtttt cgaactggct atacatcgcc ttctggatgt tgtcgaagtt
    33001 cttgtctttc gccagcttgt caaactcacc gcccaggtca tcttcccagt tcggcccttt
    33061 ttcgattttc gccatccgtt cgccggtaat cagtgaacgc ggacgggcaa tcggctgtga
    33121 tttgctgcct tccggcgcgg tatgcaggtt ctgataatca aaatcgaacg gctcgtaata
    33181 atcgtcgatc cccggcagat gcgggttagc gaagatttta cccagcagca tggcacggtt
    33241 acccatgcgc ggctgcagtt tgccgttgat tttacggatc cagccgcctt tgtatttttc
    33301 ctggttttcc cagtcagtcg ggaagccctg gcccggcttg gtttccacgt tgttgaacca
    33361 cgcgtattcc acgccttcac ggctggtcca gacgttttta caggtcactg aacaggtgtg
    33421 gcagccgatg cacttatcga gattcagcac catgccgact tgtgaacgaa ttttcatttt
    33481 acgctctcct gtacctggtc attgccttcg ccatctaacc agtcaatgtt cttcatctta
    33541 cgcactacaa caaactcatc gcggttagaa cctacggtgc cgtagtagtt aaagccatat
    33601 gccagatggg catagccgcc gatcatatgc gtcggtttcg gcgtgatacg ggtgaccgag
    33661 ttatggatac caccacgctg ttgggtaatt tccgaaccag gcaggttaac gatacgttcc
    33721 tgcgcgtggt acatcatggt catccctgcc ggaacacgct ggctgacaac cgcacgggca
    33781 gtcagtgcac cgttgctgtt gaagacttca atccagtcgt tatcggcgat acccagctct
    33841 ttggcatcgg cttcactcag ccagaccacc ggaccaccgc gacctaaagt cagcatcagc
    33901 aggttgtcgc tgtaggtgga gtggataccc cacttctggt gcggcgtcag gaagttgagc
    33961 gctttttccg ggttgccgtt ggatttctgc cccatcactt ctttcaccga acgggtgtcg
    34021 atcggcggac gataaaccag caggctttca ccgaaatcac gcatccactg gtgatcctga
    34081 tacagttgct gacgaccaga gagcgtacgc cacgggatca gctcgtgaac gttggtgtaa
    34141 ccggcgttgt aagaaacgtg ttcatcttca aggccagacc atgtcgggct ggagatgatt
    34201 ttgcgcggct gtgcctgaat atcgcggaag cgaatcttct cgtcttcttt attcagcgcc
    34261 agatgcgtat ggtcacggcc ggtaaactcg ctcagggcag cccaggcttt tacagctacc
    34321 tgaccattgg tttccggtgc cagcgtcagg atcatctctg ccgcatcaat tgcggtattg
    34381 agcatcggct ggccttttgc cggaccttcc gctttggtgt agttgagctt acgcagcaga
    34441 tccatctcgc tctgggtgtt ccaggcaatc cctttaccgc cgttaccgat tttctccatc
    34501 agcgggccga tagaggtaaa gcgttcgtaa gttgctggat aatcgcgctc tacgaccata
    34561 atgtgcggtg cagttttacc tgggatcagg tcgcattcgc cttttttcca gtctttcaca
    34621 tccagcggtt gcgccagttc agcggcagag tcgtgctgga taggcagcgt gacgatgtca
    34681 gtttctttac ccagatgacc aacgcacact tcggagaatt tcttcgcgat ggctttgtag
    34741 atttcccagt cgcttttcgc ttcccaggcc ggatcgaccg ccgcagacag cgggtgaata
    34801 aacggatgca tatccgaagt attcatgtcg tctttttcgt accaggtcgc cgtcggcaaa
    34861 atgatgtcgg aatagagaca ggtgctcgac agacggaagt ccagcgtcac caccagatcc
    34921 agcttgcctt ccagaccgtt gtcctgccag tccacttctt ccggcttcac accgccctgc
    34981 tgccccaggt ctttgccctg gataccgtgc tccgtcccca gcaggtactt gagcataaac
    35041 tcatgacctt tactggaaga accgagcagg ttagaacgcc agatgaacag attacgcggg
    35101 tggtttttac cgttttccgg ctgttccgca gcgaaacgga tagaaccctc tttcagggat
    35161 ttaaccgtat agtccaccgg attcatcccg gctttttctg cttcgcgagc gatagtcagc
    35221 gggttagtgc ctaactgcgg cgcagacggc agccagccca tgcgttctgc acggacgtta
    35281 aagtcgatca agtgtccggt atagcgagat ttatccgcca tcggcgacag cagttcttcc
    35341 gcagtcacgg tttcataacg ccactggctg gagtggttat agaaataaga ggtactgttc
    35401 atgtgacgcg ccggacgctg ccagtcgagg gcaaacgcca gcggctgcca gccggtttgc
    35461 ggacgcagtt tttcctgacc tacatagtgc gcccagccac cgccgctctg accgacacag
    35521 ccgcagaaaa tcagcatgtt gatcagacca cgatagttca tgtccaggtg ataccagtgg
    35581 ttcagcccag cgccgacgat aatcatcgaa cgaccgtgcg ttttatcagc gttatcggca
    35641 aattcacggg cgatgcgaat aatctggctg cgagaaacgc cggtaatctg ctctgcccag
    35701 gcaggggtgt aagctttcac atcgtcatag ctggttgcac agttaacgtc gttcaggcca
    35761 cgttccagac cgtagtttgc cagcgtcaga tcataaacgg tggtcaccag ggcggtgcta
    35821 ccatcagcca gttgcaggcg tttcaccggc agcttatgca gcagcacgtt ttccagttcc
    35881 actttgttga agtgttccgt gccatcgcca ccaaagtacg ggaagcccac ctcagcgatc
    35941 tcatcctggc tacccagcag gctcagttgc agctcggttt cttcgccagt tttgccgtcg
    36001 cgctgctcaa gattccattt gcccttctcg ccccagcgga agccaataga accgttcggc
    36061 gcaaccattt caccattggt attaaaggcg acagttttcc attccggatt gttttcctgg
    36121 cccagtgcat ccaccagatc agcagcgcgc agcatacgac ccgcagcgta gtaaccgtcg
    36181 cgctcttcca gcatcaccag catcggcatg tcggtgtagc ggcgcacata gtcggtgaaa
    36241 tactggcttg ggttgtcgag gtggaattca cgcagcatta cgtgacccat tgccagcgcc
    36301 attgccgcat cggtgccctg tttcggtgcc agccacagat cgcacagttt ggcgatttca
    36361 gcgtagtctg gtgtgacggc aacagttttg gtccctttgt aacgcacttc agtaaagaag
    36421 tgagcatccg gggtacgcgt ctgcggcacg tttgaacccc aggcgatgat gtagctggag
    36481 ttgtaccagt cagcagattc cggtacgtca gtttgctcac cccaggtttg cggagacgca
    36541 ggaggcaggt cgcagtacca gtcgtagaag cttaagcaag taccgccaat cagcgagaga
    36601 tagcgcgcac ccgatgcgta agaaaccatc gacattgccg gaattggcga gaagccagcg
    36661 acacggtccg ggccgtaatt tttgatggtg taaacgttag atgcggcgat cagttcgttc
    36721 acttcctgcc aggaagaacg aacaaaacca ccgcgtccac gcgcctgctt aaagcttttc
    36781 gctttatcgg cgtcttcaat gatagaagcc catgcctcaa ccggatcgct atgcagcgcc
    36841 ttcgcttcac gccacatttt catcaggcgt ttgcgcatca tcgggtattt caggcggtta
    36901 gcgctgtaaa gataccagga gtagctggca ccgcgcgggc agccgcgagg ttcatggttt
    36961 ggcaggtccg ggcgagtacg cggatagtca gtctgctggg tttcccaggt gaccagaccg
    37021 tttttgacgt agattttcca gctgcaggag ccggtgcagt ttaccccgtg ggtagagcgg
    37081 acgattttgt catgctgcca acgctggcga tatccatcct cccagtcacg gttggtattg
    37141 agaagctggc catgcccatc ggcaaaggtt tcacccttct gtttgaagta gcgaaaccgg
    37201 tccaggaatt tactcatcgg ttttctcctg tgggagcctg tcggccctct gataaatcga
    37261 cattgctaat gtttaagggc aaggtaacgc tctgaaacag cgtgggaatt gataacgatc
    37321 aagagtgatg gagatgaaaa ataaagtaaa ttcctggaat acccctttag gataattaac
    37381 atttatagca taacagactg taaattaaga cgttttacac ctgtagttat ttaaaccatt
    37441 aagattttta tgcagatggg tattaaggag tattccccat gggtaacata ttaatgactc
    37501 ttaatgaaat ccagcgaaat aagccataca acattagtat gttgtaacta aatgacgtat
    37561 aaaaaaagcg cgacatcatg ccgcgcaaag gataatcaaa tattacgatt tattttttag
    37621 aatgccgacc atataccgcc caggtaatca ccacgcaggc gatatagaaa atcaaaaata
    37681 ctttcattgc gccgactggc gaacccgtta atgccagcga gctaccaaac gctttcggga
    37741 taaagaagcc accaatcgcg ccaatcgcag agatgaaacc cagcgccgct gccgtgtcgg
    37801 ttgccgcttc acgcatcgca cgttcgtcag aacccccttc tgctttcacg cgatccattg
    37861 tcagtttacg gaagatcacg gaaatcatct ggaaagtgga accactaccc agcccagcgg
    37921 tcaggaacag cgccaggaag actgcgaaga acgccatgaa gcttccgccc tgtccgtcag
    37981 tcggtaaggt caggaacagc aggccgctga aaatcgccat caggataaag ttcaccagcg
    38041 tgacacgagt tccgcccaga cggtcagata atgcaccacc tgcagaacgc gccagcgcac
    38101 caataaacgg cccgaagaaa gcgtattgca gaatctgaac atccgggaac tgcgtttttg
    38161 acagcatcgc aaagcccgcg gagaagccga tgaaggagcc gaaggttgcc agatacagca
    38221 ggctcataat ccacagatga ccccgtttga gtaccggcaa ctgctccttg atggaggctt
    38281 tcgaggtggc aagatcgttc atgccaaacc acgccgcaat ggtgaagatg gcaagtaacg
    38341 gcacccatat ccaggacgca ttcgccagat acagctcagt cccatccggc tgtttgacgc
    38401 cctggctacc aaatactgcg aaaatcgaca gtgataccac cagcggagca accaactgca
    38461 tgacgctgac gcccatgttg cccagacccc cattcagacc cagcgcgcca ccctgcttct
    38521 gtttcggaaa gaagaagctg atgtttgcca tactggatgc gaagttcgcg ccagcaaagc
    38581 cgcacagcag agaaatgatg atgaagacgc tataaggcgt ggaggtatcc tgcacggcaa
    38641 aacccagcca gacgcaagga ataatcagaa taccggtgct gaacgccgtc cagcgacgac
    38701 caccgaagat aggaaccata aaggagtatg gaacacgtaa taacgcgcca gaaaccgaag
    38761 gcagcgcagt caacataaat agctgatcgg tcgtaaaatt aaaaccgact ttcggtaagt
    38821 tcaccgcaac agcgctgaac aacatccata cgcaaaacgc cagcagcaga cagggaacgg
    38881 aaatccacag gttgcggctg gcaatacgtt gaccgcgttg ttgccagaac gcaggatctt
    38941 ccggtcgcca atctgtaatg acagctccag tagccctttc gggggcggat gagtgactca
    39001 tagacacctc tgatactcgt ttcgatgtct gccaccttag tgtctgtagc taaaggcaat
    39061 ttgatgtaaa tcaaacgata agataacttt atctatcatt gatatttatc attatccata
    39121 gtgagtacag tgatttcata aaaattatga gatttttcac ggtactgtaa aatccctacc
    39181 cttaccgatg taaagcgact aaccacacgg caaataagga gtaactcttt ccgggtatgg
    39241 gtatacttca gccaatagcc gagaatactg ccattccaga atgtatcgtc acattcatta
    39301 aggttattgc tcatttaaag cctgaaggaa gaggtttaca tgcttaaacg ttgtctctct
    39361 ccgctcaccc tggttaatca ggttgcgctt attgtgttgc tttctactgc tattggactg
    39421 gcagggatgg cggtttctgg ctggctggtg caaggcgttc agggcagcgc ccatgcgatc
    39481 aacaaagcgg gatcgctgcg catgcaaagt taccgtctgt tggcagcagt gccattaagc
    39541 gagaaagaca agcccttaat taaagagatg gaacaaacag catttagcgc cgagttgact
    39601 cgagcagcag aacgagacgg gcaactggcg caattacagg gtttacaaga ttactggcgt
    39661 aatgaactga tccctgcact gatacgtgca caaaaccgcg aaacggtgtc agcggatgtc
    39721 agccagtttg ttgccgggct tgatcagctg gtatctggtt ttgatcgcac cacggaaatg
    39781 cgcattgaga cagtggtgct ggtccatcgg gtaatggcgg tatttatggc acttttactg
    39841 gtattcacta ttatctggtt gcgggcgaga ttgctacaac cgtggcggca actgctggca
    39901 atggcgagtg ccgtcagcca tcgcgatttt acccaacgcg caaacaccag cgggcgcaac
    39961 gaaatggcga tgcttggaac tgcgttgaac aatatgtctg cagaactggc cgaaagttat
    40021 gccgtacttg agcagcgggt tcaggagaaa accgccgggc tggagcataa aaatcagatc
    40081 ctctcttttt tatggcaggc taaccgccgt ttgcattccc gcgccccgct gtgtgaacgc
    40141 ctgtcacctg tactcaacgg attacagaat ttaaccctgc tacgtgatat cgaactgcgg
    40201 gtgtatgaca ctgatgatga agagaatcat caggagttta cctgccagcc agatatgact
    40261 tgtgatgata aaggctgcca gctctgcccg cgcggcgtat tacccgttgg cgatcgcggc
    40321 acaaccctga agtggcggct ggctgacact catacgcagt acggtatttt gctggcgacc
    40381 ctgccgcagg ggcgtcatct tagccatgat caacaacaac tggtggatac cctggttgaa
    40441 caactcaccg ccacgctggc gctggatcgc catcaggaac gtcagcaaca gttgatcgtg
    40501 atggaagagc gtgccaccat tgcgcgcgaa ctgcatgatt ctattgccca atctctctct
    40561 tgcatgaaga tgcaggtgag ttgcttacag atgcagggcg atgcgctgcc agaaagcagc
    40621 cgcgaactgt taagtcagat ccgtaacgaa ctgaatgcat cctgggtgca gttgcgtgaa
    40681 ttgctcacca cattccgttt gcagctcacc gagcctggat tacgtccggc gctggaggcg
    40741 agttgcgaag agtacagcgc caaatttggc ttcccggtga agctggatta tcaattgccg
    40801 cctcgtctgg tgccttcgca tcaggcaatc cacttgttgc aaattgcccg tgaggcatta
    40861 agtaacgccc tcaaacattc gcaagcgagt gaggtcgtgg tgacggtggc gcaaaacgat
    40921 aatcaggtca aactgaccgt ccaggataac ggctgcggcg tgcctgaaaa tgccatccgc
    40981 agcaatcact acggcatgat aataatgcgc gaccgtgcgc aaagtttacg aggcgattgc
    41041 cgcgtccgcc gtcgtgaatc aggtggcacc gaagtggtgg tcacctttat tcccgaaaaa
    41101 actttcacag acgtccaagg agatacccat gagtaatcag gaaccggcta ctatcctgct
    41161 gatcgacgat cacccgatgt tgcgaactgg cgtaaaacag cttatcagta tggcaccaga
    41221 tatcaccgtg gttggcgaag cgagtaatgg cgaacagggt attgaactgg cggagtctct
    41281 tgatcccgat ctgatcctgt tagatctcaa tatgcccggc atgaacggtc tggaaacgct
    41341 ggataaactg cgcgaaaagt ccctctccgg gcgcattgtg gtattcagcg tctctaacca
    41401 tgaagaagat gtggtcaccg cactgaaacg cggcgcggat ggctatctgt taaaagatat
    41461 ggaaccggaa gatctgctga aagcgttaca tcaggcagct gctggcgaaa tggtattaag
    41521 cgaagcatta acgcctgttc tggccgccag cttgcgcgct aaccgtgcca ctactgagcg
    41581 cgatgttaac cagttaaccc cacgcgagcg cgatattctc aagctaattg cccagggttt
    41641 gccgaacaag atgattgccc gccgcctgga catcaccgaa agcacagtaa aagttcacgt
    41701 caagcacatg ctgaagaaaa tgaagctaaa gtctcgcgtg gaagctgcgg tatgggtgca
    41761 tcaggagcgc attttctgat tacggttccc agcgcagttc gtcgtttgac aatgcgtcga
    41821 acggttcgac aagcgttagc gtgatctcat tggaggagac acgctgcccc tggttatctt
    41881 ccaccaccac tgacaatcgc cagtgattgc ttgcgccttc cccgttttgc cagtcaggca
    41941 tgatcagcgt ccagccctcc tcactgttgg cttgtgcgcc cggcgtcaaa cttaatatct
    42001 gcgtatcgcc ctgccaaatc agttgccgaa taccgtaacg actgcggatt tgtaatttca
    42061 gcggcactgt ttcgccaggt tttagatccc acggcggtgt cgccagaaac accgtcaacg
    42121 tttttcgctg tcggtactca agagtcggaa gattatttcg ctgcggattg tcatagcgac
    42181 taccacgtaa cgactgactt tcggcaacct cgcccgcaga aagttgtttt ttgagcggta
    42241 caccaaagcg gtagttaaga ttcagcccga ggttattttg attctcgcca ctttcaccct
    42301 gtttatgctg ggccgtcaca gtgactaagg gcacaggggt gtaatttaat cccagactca
    42361 acgcgacggg attgtgataa cccgtaccag agttaaacaa atcgacacga tcaccaaaat
    42421 actgttctac gctgacactg gtattgaggt gttgatagaa cggcatgcgc atccgggctg
    42481 tcaggtcgta cccgcgcgcc atccgttgtt cctgcgtggc tgtctgttca tgccatgcag
    42541 caaacggctg ataaaagttt gccgatagtc gcaaatattc gccccacgct tcagcgccaa
    42601 agcccgctcg ctgaagattt tcgtccagca agttgtcata aaaagtgtta taacccacca
    42661 gccagctgcc gcgcgcccag cgttgcccaa cgtccacatt gctcaccagc ccatcatcct
    42721 gctgagtaag accaagctgg ctccaggtga gataacgatc attatcttgt aacggcacaa
    42781 accagcttcc acgactgccg gtgaaatgtc cttcgttatc cactttgacg tcaacactgg
    42841 catttcccca cggtgatagc caggactcta cgtgctgatt aacctgttga ctaagcgcgt
    42901 cgcggacctt tcccaatgcg aaagcttttg cctgctcgcc agtatcaagc ccgttatcat
    42961 tcatactggt ttcgccgaaa tctttcacaa tttcagcaaa gtgtttttcc ccatcatgat
    43021 tttcaggtgc catgcctaaa tccggcagac catcattgtt attatcaaag ggatttgccg
    43081 ctttttgctc gaaggtagat tgtgcgttag ctgtaccgcc tgccgccagc aagagtaaat
    43141 aaaacggaat aatgcgagga atgaaacggc tcaaaacgtc ggtaacttcc ggtaaaaatg
    43201 aaaaaccata gccttaaaga ataacgatta ctggcacctt ttgtcgtatt ttctcgcgct
    43261 ttcaggaaaa tccttaaaaa tgtgaccgat tggtcagacg ctgcagaagc agtaagatag
    43321 aagatgagtg tctaattccc tgaatacagg agtaaataat gcaaaaaatc gtgatcgttg
    43381 ccaatggcgc accttacggg agcgaatcct tgtttaacag cttgcggctg gccattgcgt
    43441 tacgagagca ggagaacaat ctggatctgc gtctgttcct gatgtctgat gcggtaacag
    43501 ccgggttgcg cgggcaaaaa ccaggggaag gctacaacat tcaacaaatg ctggagatcc
    43561 ttaccgctca gaatgtaccg gtgaaattgt gcaaaacctg taccgacggg cgcgggatta
    43621 gtacacttcc tctgattgat ggggtggaaa tcggtactct tgttgaactg gcgcaatgga
    43681 cgctgtcagc cgataaagtg ctcacatttt aatgtttgag ggggcgtcgg acgccccact
    43741 atgaggaaag tttactcagg ctaatcccgg ccgtaacaca ccatccggat aattctccgc
    43801 cagcagtttt tttaccgata ccagcaattc attcagccca tcgtcctgca ttcccagttt
    43861 gataagctcc tgttccagtg aaaacaggta ttgtgcattg gttcccagcg gaccgctcgc
    43921 cgccgcaatc aacggcgcta tgacctgagc gcgagtgtca gattcatatt ctggatgtcg
    43981 cgggtccata ataaacacaa tggcgtttac tgtgcgtcca tcatcaagat caagctgaca
    44041 ccaggttggc agataacagc cggtaatcat ctcgcgcttc cacagcaggg ttagttcctg
    44101 ctccagcgtc tcttctggca gtcgataggc gacgcctgtg gtgcgtccgc cctctttcag
    44161 tgcaagcatt cgtcccggct gctgcgcagt cccgcgcccg gcggtcaggc gcaggcaaaa
    44221 cgcgcgatgc catccaacca gtgtaccggt gcacgactcg gtaaactcca gtgccggatt
    44281 ccacatcaac gatccataac cgaagatcca caccggtccc tcatcaggtc gacaagccag
    44341 cgtcgccgcc agcgaagccg cccgttgttc tgctgaccat aagagtgatt cttcaatggc
    44401 accaaatgcc gttttacaat cggcattcat caagaaatca cgcgttatca ccttacacct
    44461 ccgccactgc atacttccct gcgacaactt tttggtgtct tccggacatt tttactgctc
    44521 attttgaaac cactaacggg acaatatgca atccacggac cacttgcaag gcaacaccgc
    44581 acgaatagct ttaactaagc taccggtttt acgatttttt atgccattta tcatcatccc
    44641 ctttggcata ttcatgcttc acagcagccc aggccacttt atgcgctgtt tcttcgcgac
    44701 tggcgtcatc acgccgatct tctttatctt tatattgatc ccatgcgctg ttgaacgctt
    44761 ctttatagat atcctgggca tgagacggaa gcacgtgctt tacgctttcc ggcagatcgc
    44821 ttttcgtttt atacggcatc gttgactcct tttacaaagt tgaacattaa gtgtggtcaa
    44881 caatatctac tcttgccaaa acataagaat tttcatgtca gactcttctt aagaagtgct
    44941 aaaacaaaca ttgtctaaac gtattactta tttttatgcc caaatgagta aaaaatcgac
    45001 agcggggtaa aaattcgtaa aatgttaccg atatccagac actttctgtt atttttccta
    45061 cctatttttt acattttcga taaattcatc tgcaaaggga taaccataat gtcaaatgct
    45121 caagaggcgg taaaaacccg ccacaaggag acttcgctta ttttcccggt tctggcgctg
    45181 gtagtgctgt tcctgtgggg aagcagccag acactaccag tggtcattgc catcaatctt
    45241 cttgcgctta ttggtatttt aagtagcgcc tttagtgttg tccgtcatgc ggacgtatta
    45301 gcccatcgcc tgggagaacc ttacggttcg cttattctta gcctttcagt ggttattctt
    45361 gaagtcagtt tgatttcagc tttaatggca accggcgacg ccgcgccaac gctaatgcgt
    45421 gatacgctct attcaatcat tatgattgtt acaggtgggc tggttggctt ttcattattg
    45481 ttgggcggac gtaagtttgc cacccaatat atgaatctgt ttggtatcaa gcagtattta
    45541 attgcgctgt tccccctggc gataatcgta ctggtatttc caatggcgct gcctgcggcg
    45601 aatttttcaa caggtcaggc gttactggta gcattaattt ctgcggcaat gtatggcgta
    45661 tttttgctga tccagaccaa aacgcatcaa agtttgtttg tctacgagca cgaagatgac
    45721 agtgatgatg acgacccgca ccacggtaaa ccgtctgccc atagcagcct gtggcatgcc
    45781 atctggttaa ttgtccatct gattgccgtt attgcagtca ccaaaatgaa cgccagcccg
    45841 ctggagacat tgctcgacag catgaatgcg cctgtcgcct ttactggctt cctggtggct
    45901 ctgttgattc tgtcgccgga aggtttaggt gcgttaaaag cagtgttgaa taaccaggtc
    45961 cagcgcgcga tgaatctgtt ctttggttca gtgttggcaa ccatttcgct taccgtacct
    46021 gtcgtcacgc taattgcctt tatgacgggt aacgaattgc agtttgcact tggtgcgcca
    46081 gaaatggtgg tgatggtggc ctctttagta ctgtgccata tctccttctc caccggacga
    46141 actaacgtgc tcaatggcgc agcgcatctg gcactgtttg ccgcctattt gatgacgata
    46201 tttgcctgac agttatatct cctccgatga cggaaatcgg gggagataat ataaattcct
    46261 gcttattgta tttcttcata ctacctggac aggtcgttaa tcagaattgc gagcctgtac
    46321 cacaacctgc acgaaaccac tgcaacagct gttgaaaagc cggaatgtac tcttcaatgc
    46381 aaacatttca gggatgaagt gaagatgaat tgcgacgtag tatccacacc aagccggcgt
    46441 tatccggtga ggcgcaatgt tgcgggggct ttatccctgg tggcattggt tgctggaaag
    46501 agaaaacccc cgcacgttga gaggtaaaac ctgacaacag aacgggggct aatcttgaac
    46561 catcaacaat tcaagaatag ccgcaaaatg ttgtcattac aacaaggcgg ctatatgacg
    46621 ctcgcgcagt ttgccatgat tttctggcac gacctggcag caccgatcct ggcgggaatt
    46681 attaccgcag cgattgtcgg ctggtggcgt aaccggaagt aaggtgaaaa cgggcgtcag
    46741 gctgccgtga tggcaatatg cgcccgaaac cggccgcagg ggtaacagcc tgcggccttt
    46801 tttgtatccg gcccccataa aaaaagtctt aacgcagaac gctaagactt tatttttcaa
    46861 acaaaaagat tacttgctgg tatccagttc ttcgaaacct ttcaccagat catcaatcgc
    46921 tttcatttgc ttgaggaacg gttccagttt agccagcggc agcgcggatg gaccatcaca
    46981 tttcgcatgt tccggatccg gatgcgcttc aataaacagc cccgccagac ctaccgccat
    47041 accggctctt gccagctcag ccacctgagc acggcgaccg ccggaagctg cgccaaatgg
    47101 atcgcgacat tgcagtgcgt gggtaacgtc gaaaatcacc ggcgaattac cagacacttt
    47161 cttcatgatg ctgaagccca gcatatcaac aaccaggttg tcatagccga agttagcacc
    47221 gcgatcgcaa agaatcactt tttcgttgcc gccttctttg aatttatcaa cgatattacc
    47281 catctgtccc gggctgacaa actgcggttt cttgacgtta attaccgcac cggttttcgc
    47341 catggcttca accaggtcag tctggcgagc aagaaacgcc ggcaactgaa tcacatccac
    47401 gacatcagca acgggctgtg cctgacttgg ttcgtgaacg tcggtgataa ttttcacgcc
    47461 aaaagtctgc ttcaactcct ggaagatttt catcccttct tccaggcccg gtccacgata
    47521 agagtggatg gaggagcggt tggctttgtc aaaagaggct ttgaacacgt aagggatacc
    47581 cagtttctgg gtcacggtta cgtagtgctc gcaaatgcgc atcgccagat cgcgagattc
    47641 cagcacgttc ataccgccaa acagtacgaa cggcaggtca tttgctacgt tgatgtcgcc
    47701 aatgctaacc actttttgtt tcataggatc gccttaatct tgagtaaaat gtcgattaat
    47761 taatgcagca caatatgttt atgcgcgatg ttatttattt gcgcacggat catttcgctg
    47821 atcgggtctt ccggacactg ttcaacgaaa taacttaaat cgttcaacgc aacgtgttcg
    47881 caatccagtt gcgcataaat caacccgcga tcgcgaattt catagggatc ttccgggttg
    47941 aattgtaata aagcttcgct ggtgcgtaac gccagctcca tctgattttc ttccatcaac
    48001 gaagctttga gtgtatccag caatttgcgg attacctcaa tgttatcagc ttcatcaagg
    48061 tcttcataaa acagttccgc cgacgggctg atatttccct ttaaccacac atccagcata
    48121 tgttcgctta acgattcacc gttaaaagga ttaatcagcc aaatttcgcc atccggacat
    48181 tcaatgcgca atatcagctg cgtagggaaa atcaccggca gcagcggcaa atcgagacga
    48241 ttcgcgaccc ataataaaac cgcacctaat gataccgcac tgccctgtcg attctttaac
    48301 acctggtcca gccataatgc atcggaaaga cgataaacgc cgcgtgaggc tttaaacccc
    48361 cattcgccgt aaaacagcgc gattaatttt tctagctgct cttctaatgg cagaagctgg
    48421 ctaatttctt ccttcgccag actaacgaga cgctccagtt cgtcgtaaac atcttgcgag
    48481 ggaaaatcgc ggcggattgc ttcgcaagcc aggatcatgc cttcgcacaa tggcgcttta
    48541 ttaaattcga aatcagctaa cgatctcatg acttacccca gtaacggtac ttttgtggtg
    48601 gcgagtttaa tgatgatgta cagcaccacc aacgccagcg ggaaggcaat aatgcgcgcc
    48661 tgctgactgt gcatacgacg ataatcgagt gcaataaaac ccaaaacaat ataaatgata
    48721 actccaaaca gcttttcagt cagccactgt gcctgcccgg aaaatggctg gatgtgcgct
    48781 ttagccatca acgcaatgcc gcttaacaat aacagcgtat cgacaaccgg cggcacaatg
    48841 cgcgtccagc gagcaaatgc ctgtggatgc ttctgataac gtagccagaa acgtaaggtc
    48901 aatagcccaa cagaaagcgc gatactaatg agatgaacgc taagcaacgt agaaaaactt
    48961 gtcattgata atagcggcca agcgttacgc gctcgttatc cccatagtca cggcaggttt
    49021 cgacgtcatg ataccccgcg aagataaatg cctgtcgcac cgcttcaccc tgctgccagc
    49081 catgctccag aagcagaaag ccgccggata ccagcgcgtt acgcgactgt tcgatgatat
    49141 gcacgatgtc tgccattcca ctgtctgccg caaccagcgc agtgagcggc tcaaagcgga
    49201 catcgccttg ttgaagatgt gggtcctgct cgtcaatata cggcggattg ctgacaatca
    49261 tcgcaaactg ctgcccggct agcgcgctaa accagtcgct ttgcagaatg tggatatttt
    49321 tgatagccag atgctgggca ttacgttgcg ccagagagac agcatctggc atacgatcta
    49381 cagcggtaat ttcgcagtcc ggacgctcgc tggccagcgc cagcgcaatc gccccggtac
    49441 ccgtcccgag atcgagaata cggcaaggtt gttcaggcaa ccgcgccagt gcctgctcca
    49501 ccagacactc cgtatccggg cgcggaatta aggtcgctgg cgaaacaaat aacggcaacg
    49561 accagaattc tcgcaccccg gttaaatgag caatgggttc accatcgcga cgacgcgtca
    49621 gtagcgcatc aagttgctga cattgttcgt cagtcagctg cgtttcacca aaggcgagaa
    49681 taaaagtacg ccctctgccg gtaacatgct ccagcaggat ttcagcatca cgccgcgggc
    49741 tttcgctcgc ctgaagttgg cttattgctt cacgtaacca gtgttgatat tccattattc
    49801 ctgctcggac aacgccgcca gttggtcggc ctgatgttcc tggataatcg gttcaatcag
    49861 catatccagc ttaccttcca tcacttcatc caggcggtag agcgtcaggt tgatgcggtg
    49921 atcggtaacg cgcccctgcg ggaagttgta agtacggtta cggtcgctgc gatcgccact
    49981 ccccagcagg ttacgacggg tagacgcttc ggcctgttgc cgttttgcca tttcagcagc
    50041 gtggatgcga gcaccgagta cagaaagtgc tttagctttg tttttatgtt gtgaacgttc
    50101 gtcctgacat tcaacaacaa tcccggtcgg caagtgagta atacgaattg ccgaatcggt
    50161 ggtgttaacg tgctgaccac ccgcccctga cgagcggaaa gtatcaatgc gtaaatctgc
    50221 tgggttgatg tccggcagtt ctgcatcagg cagttctggc attaccgcaa cggtacaagc
    50281 agaagtatga atacgaccct gcgattccgt agcaggaaca cgctgcacgc gatgaccgcc
    50341 agattcgaat ttcaaacgac catatacacc atcaccgcta attttggcga tgatctcttt
    50401 ataaccacca tgttcaccct cgctggcgct catgatttct acacgccagc ggcgggcttc
    50461 ggcgtaacgg ctgtacatac ggaacagatc gccagcgaac agcgccgctt cgtcgccgcc
    50521 ggttccggca cggacttcga ggaaggcgtt acgttcgtca tcaggatctt ttggtagtaa
    50581 cagaacctgt aattgctgtt ccagttgctc gcttttttct ttagcttcgc gcagttcatc
    50641 ctgcgccatc tcacgcattt caggatcgtc gagcatcatc tgtgcggttt cgatatcttc
    50701 ctgaacctgt tgccagtcgg taaaacagcg cgaaacatca cttaactgcg catattcacg
    50761 agataatgcg cgaaaacgtt cctggtcagc gatggtttgc gcatcaccca gcagcgcctg
    50821 aacttcttca tggcgttcat gcagggcttc cagtttggca acgatagaag gcttcatagg
    50881 cgtaaatgca ccctgtaaaa aaagaaaatg atgtactgct actccagccc gaggctgtcg
    50941 cgcagaatat tcaggcgttc gttatccccg tcacgggcgg cctgttgaag tgattttgtt
    51001 ggtgcatgga tcaggcggtt agtcagtttc catgccagat cctgcataat ggtttgcgca
    51061 tcaccgccct gttcaagggc cgctaacgct ttagcagtta actcatcacg aacctgctct
    51121 gcctggctac gataatcgcg aatagtttcg ctggcgcttt gtgctcgaag ccatgccata
    51181 aattcactgg cctcctgggc gacgatagtt tccgcctcca cagcagcagc tttccgttgc
    51241 gccaggttgt gcgaaataat gctttgcaga tcgtcaacgc tataaagata agcattcgcc
    51301 agtttaccaa cttccggctc aacatcgcgc ggaacggcaa tatccaccag cagcattggt
    51361 tgattgcgac ggctttttaa tgcgcgctcc accatgcctt taccgataat cggcaacggg
    51421 ctggcggtgg aactgatgat gatatcggct tcgcgcagac gttcgtcgat atcgctcagg
    51481 gcaatcactt ccgcgccaac ctcatctgcc agaatttggg cacgttcgcg agtgcgattg
    51541 gcgataatca tcttctgtac tttgtgttca cgcagatgac gcgccaccag ctcgatggtt
    51601 tcgcccgcgc caaccagcag taccgtcacc gtaaagagcg attcaaagat ctgccgcgcc
    51661 agcgtacaag ccgcaaaagc gacagacaca gcactggcac cgatatctgt ttcagtgcga
    51721 acgcgtttcg ccacagagaa agatttctga aacatgcgtt ccagttcgct ggccttcata
    51781 tgaccttttt gcgaatcggc aaacgctttt ttaacctgac cgaggatctg cggctcgcca
    51841 aggacgagag aatcaagccc actggcaacg cgcattaaat gactgaccgc atcgttatcc
    51901 tgatgccagt agaggctatt acgcagatcg tcttcgttga gattatggta atcacacaac
    51961 cagcgaataa gcgcctcttg caggtcatcc cgttcttcaa cgctgagata aagttctgtg
    52021 cggttacacg tcgacagcac cacaccgccc tgcaccatcg gctgcgcgag cagactgtca
    52081 agcgcctgat cgagcttatc cggcgaaaac gatacacgtt ctcgcagcga tacaggtgcc
    52141 gttttatggt tgataccgag tgctaaaagg gtcatgtctg cgggaaataa taccaacgtt
    52201 gatagggtta gtctgcttgc atcatacagg atgcgtaaga tcaataaaag agagcgcccc
    52261 cttttggagt aattgccgaa agccgttaga ttctggcaat taagacaacg tgaacataga
    52321 cgatggctaa cggtaacgct agcattaagg gttataactg caacgtatct caaggacttg
    52381 tcattattat gcccctgccc gattttcgct ttatccgcct gctaccgcta gcagccctcg
    52441 tgctcactgc ctgttctgtt accacaccca aaggcccagg caaaagcccg gattcgccac
    52501 aatggcgtca gcatcagcaa gacgtgcgca atcttaatca gtatcaaact cgcggcgcgt
    52561 tcgcttatat ttctgaccaa caaaaagtgt acgcccgttt cttctggcag caaactggcc
    52621 aggatcgcta ccgtctgctg ctgactaacc cattgggcag cacggaactg gagctgaatg
    52681 ctcaaccggg taacgtgcag ttagtcgaca ataaaggtca gcgttatact gccgatgacg
    52741 ccgaagagat gattggcaaa ttgaccggaa tgccaattcc gctcaacagc ttgcgccagt
    52801 ggattttagg tttaccgggt gatgcgactg actacaaact tgacgaccag taccgcctga
    52861 gtgaaatcac ctacagccag aatggcaaaa actggaaagt tgtttacggt ggttatgaca
    52921 caaaaactca acctgcgatg ccagccaata tggaactcac cgacggtggt caacgcatca
    52981 agttaaaaat ggataactgg atagtgaaat aatgcggaca cagtggccct ctccggcaaa
    53041 acttaatctg tttttataca ttaccggtca gcgtgcggat ggttaccaca cgctgcaaac
    53101 gctgtttcag tttcttgatt acggcgacac catcagcatt gagcttcgtg acgatgggga
    53161 tattcgtctg ttaacgcccg ttgaaggcgt ggaacatgaa gataacctga tcgttcgcgc
    53221 agcgaggttg ttgatgaaaa ctgcggcaga cagcgggcgt ctttcgacgg gaagcggtgc
    53281 gaatatcagc attgacaagc gtttgccgat gggcggcggt ctcggcggtg gttcatccaa
    53341 tgccgcgacg gttctggtgg cgttaaatca tctctggcaa tgcggactaa gcatggatga
    53401 gctggcggaa atggggctga cgctgggcgc agatgttcct gtctttgttc gtggtcatgc
    53461 cgcgtttgcc gaaggcgttg gtgaaatact aacgccggtg gatccgccag agaagtggta
    53521 tctggtggcg caccctggtg taagtattcc gacaccggtg atttttaaag atcctgaact
    53581 cccgcgcaat acgccaaaaa ggtcaataga aacgttgcta aaatgtgaat tcagcaatga
    53641 ttgcgaggtt atcgcaagaa aacgttttcg cgaggttgat gcggtgcttt cctggctgtt
    53701 agaatacgcc ccgtcgcgcc tgactgggac aggggcctgt gtctttgctg aatttgatac
    53761 agagtctgaa gcccgccagg tgctagagca agccccggaa tggctcaatg gctttgtggc
    53821 gaaaggcgtt aatctttccc cattgcacag agccatgctt taagccgggc aagctgagtt
    53881 tcggtgacaa cgtcaccttg ttccagacgt tgcatcgcgc tctttaatac accgcctgga
    53941 aagaatcatg cctggcccgc acagttttcg gcagattctt tccaccaatg gacgcatgcc
    54001 tgaggttctt ctcgtgcctg atatgaagct ttttgctggt aacgccaccc cggaactagc
    54061 acaacgtatt gccaaccgcc tgtacacttc actcggcgac gccgctgtag gtcgctttag
    54121 cgacggcgaa gtcagtgtac aaattaatga aaatgtacgc ggtggtgata ttttcatcat
    54181 ccagtccact tgtgccccta ctaacgacaa cctgatggaa ttagtcgtta tggttgatgc
    54241 cctgcgtcgt gcttccgcag gtcgtatcac cgctgttatc ccctactttg gctatgcgcg
    54301 ccaggaccgt cgcgtccgtt ccgctcgtgt accaatcact gcgaaagtgg ttgcagactt
    54361 cctctccagc gtcggtgttg accgtgtgct gacagtggat ctgcacgctg aacagattca
    54421 gggtttcttc gacgttccgg ttgataacgt atttggtagc ccgatcctgc tggaagacat
    54481 gctgcagctg aatctggata acccaattgt ggtttctccg gacatcggcg gcgttgtgcg
    54541 tgcccgcgct atcgctaagc tgctgaacga taccgatatg gcaatcatcg acaaacgtcg
    54601 tccgcgtgcg aacgtttccc aggtgatgca tatcatcggt gacgttgcag gtcgtgactg
    54661 cgtactggtc gatgatatga tcgacactgg cggtacgctg tgtaaagctg ctgaagctct
    54721 gaaagaacgt ggtgctaaac gtgtatttgc gtacgcgact cacccgatct tctctggcaa
    54781 cgcggcgaac aacctgcgta actctgtaat tgatgaagtc gttgtctgcg ataccattcc
    54841 gctgagcgat gaaatcaaat cactgccgaa cgtgcgtact ctgaccctgt caggtatgct
    54901 ggccgaagcg attcgtcgta tcagcaacga agaatcgatc tctgccatgt tcgaacacta
    54961 atcgaacccg gctcaaagac ccgctgcggc gggttttttt gtctgtaata tccatttgta
    55021 tgacctatgc ctccttcacc tgccatttag ttgacagatg atgcgctcac ggatgaaaca
    55081 ttattgtgaa caaaatattt tcctcacatg tgatgccttt ccgcgctctg atcgacgctt
    55141 gctggaaaga aaaatatact gccgcacggt ttacccgtga cctgattgcc gggataaccg
    55201 tcgggattat tgctatcccg ctggcgatgg cgttggctat tggtagtggt gtggcccccc
    55261 agtacggttt atataccgca gctgttgcgg ggattgtcat tgctctgacg ggggggtcac
    55321 gctttagcgt ttccggtccg actgcggcat ttgtggtaat tctctatccc gtttcgcaac
    55381 agtttggact ggcaggactg ctggttgcga ccttgctgtc ggggatcttt ttgattctga
    55441 tggggctggc acgctttggg cgcctgattg agtatattcc ggtttccgtc accttaggtt
    55501 tcacctcggg tatcgggatc accatcggta ccatgcagat taaagatttt ctcggtctgc
    55561 aaatggccca tgtcccggaa cattatctac aaaaagtcgg cgcattattt atggcgctgc
    55621 cgaccattaa tgtgggtgat gcagccattg gcattgtgac gcttggcatt ctggttttct
    55681 ggccgcgtct gggcatacgt ttacccggcc accttccggc cttgctggct ggttgcgcgg
    55741 tgatggggat tgttaacctg ctcggcggac atgttgctac catcggttcg caattccact
    55801 acgtcctggc cgatggttct cagggtaacg gtattccgca actgctgccg caactggtgc
    55861 tgccgtggga tctgcctaat tcagaattca cgctaacctg ggattctatt cgcacactgc
    55921 tgcctgcggc attctcaatg gcaatgctcg gcgcaatcga atctctgctc tgcgccgtgg
    55981 tgctggatgg tatgaccggg acgaaacaca aggcgaacag cgaactggtt ggacagggat
    56041 tggggaatat tatcgctccg ttctttggtg gtattaccgc tacagctgcc atcgcgcgtt
    56101 ctgccgctaa cgtccgtgcc ggggcaactt cccctatctc ggcggtgatc cactctattc
    56161 tggttattct tgccctgctg gtactggcac cgctgctctc ctggctgccg ctttccgcca
    56221 tggcagccct gctgttgatg gtggcgtgga acatgagtga agcgcacaaa gtggtcgact
    56281 tgctgcgtca tgcaccgaaa gatgacatca tcgtcatgct gctgtgcatg tcgctgaccg
    56341 tgctgttcga tatggttatt gccatcagcg tggggatcgt gctggcatcg ctgctgttta
    56401 tgcgtcgtat cgcacgtatg actcgcctgg caccggtagt cgtagacgtt ccagacgatg
    56461 tcctggttct gcgcgttatt ggcccgctgt tttttgctgc cgctgaaggc ttattcacgg
    56521 acctggagtc acgtcttgaa ggcaaacgga ttgtgattct gaagtgggat gccgttccgg
    56581 tacttgatgc tggtggtctt gatgcgttcc agcgttttgt gaagcgtctg cccgaaggat
    56641 gtgaactgcg cgtgtgcaac ctggaattcc agccactgcg cactatggct cgcgcaggca
    56701 ttcaaccgat cccgggacgc ctcgcattct tcccgaatcg tcgcgcggcg atggcggatt
    56761 tataagtacg agattgacca gtcagcgcaa actgactggt cagcaaactg cattatttgt
    56821 tagctatgac ggcgattatc gctacggcgg caacgtttgt catagtggcg aacccaccag
    56881 taacggtcgc acacctgttc atgcccgcca acacgggcac ccgccagcca gagaatcgca
    56941 ccgacgaaaa tacttagcac agcaccatgt gcgaaatact ggggcatatt aaactgtggt
    57001 aactggttga ggattgaata ccccacgccg accaccatta ccaccagacc caaccccatg
    57061 agcacgttac cgagtaacga agcgtttttg cgtttcatat gtcacctccg gaactttctg
    57121 ggttgtaaca gggaataccc ctcttcctta tgtgtaaagt atagacaaca ccctgtaggt
    57181 tatgtgcggg tgtgatcaca attacaaccc ttatttcaac aaaactttac aaataaacgc
    57241 ctgaacacct tgactttcta atagtccact caggcaataa ttgcattatc tgcatttgat
    57301 acgcagtttt ttgtcaagcg gggccgcaac cagtaaacta cgcgccagtt atgtacacac
    57361 tcaggacaaa aaaacgtgac gattaaattg attgtcggcc tggcgaaccc cggtgctgaa
    57421 tacgccgcaa cgcgacataa tgctggtgcc tggttcgttg acttactggc agagcgattg
    57481 cgcgctccgc tgcgcgaaga ggctaaattc tttggttata cttcgcgagt cacccttgga
    57541 ggcgaagatg tccgcctgtt agtcccgact acatttatga atctcagcgg caaagccgtt
    57601 gcggcgatgg ccagtttttt ccgcattaat ccggacgaaa ttctggtggc ccacgacgaa
    57661 ctggatctgc ctcctggcgt cgccaaattt aagttgggtg gcggtcatgg tggtcacaat
    57721 ggactgaaag acatcatcag taaattgggt aataacccta actttcaccg tttacgcatc
    57781 ggaatcggtc atccgggcga taaaaataaa gttgtcggct ttgtgttagg caaaccgcct
    57841 gttagtgaac agaagttaat tgatgaagcc atcgacgaag cggcgcgttg tactgaaatg
    57901 tggtttacag atggcttgac caaagcgacg aaccgattgc acgcctttaa agcgcaataa
    57961 gtcgttgtct gcggcatttt tgccgagtgc cgtgtataat aggcaaagtt atttccattt
    58021 ctgcaatctg ttagcaataa caggttgatt attaagatat taaggtgatt taaatcatgg
    58081 gattcaaatg cggtatcgtc ggtttgccca acgtcgggaa atctaccctg ttcaacgcgc
    58141 tgaccaaagc cggtattgaa gcggccaact ttccattctg caccatcgag ccgaacactg
    58201 gcgtcgtacc aatgcctgac cctcgcctgg atcaactggc tgaaatcgta aaaccgcagc
    58261 gtacacttcc cacgaccatg gaatttgtcg atatcgccgg tctggtaaaa ggcgcatcga
    58321 aaggcgaagg tctgggtaac cagttcctga ccaacatccg tgaaaccgaa gcgatcggtc
    58381 acgttgttcg ctgctttgaa aatgacaaca tcattcacgt ttccggcaaa gttaacccgg
    58441 cggatgatat tgaagttatc aacaccgaac tggcgctggc ggatctcgac acctgtgaac
    58501 gtgcgattca tcgcgtacag aagaaagcca aaggtggcga taaagacgcg aaagccgagc
    58561 tggcggtact ggaaaaatgc ctgccccagc tggaaaacgc aggtatgctg cgtgcgctgg
    58621 atttaagcgc tgaagagaaa gcggctgttc gttacctgag cttcctgacg ctaaaaccaa
    58681 caatgtacat cgccaacgtc aacgaagacg gttttgaaaa caacccgtat cttgaccagg
    58741 tgcgtgaaat cgcagcgaaa gaaggttctg ttgtagttcc ggtttgtgct gctgttgaag
    58801 cagacattgc cgaactggac gacgaagagc gtgacgagtt tatgcaggag cttgggctgg
    58861 aagagccggg cctgaaccgt gtgatccgtg ccggttataa gctgctgaac ctgcaaactt
    58921 acttcaccgc tggggtgaaa gaagtgcgtg catggaccat tccggttggt gcaaccgcgc
    58981 cgcaggcagc gggcaaaatc catactgatt ttgaaaaagg ctttatccgt gcacaaacca
    59041 tctcgtttga agatttcatc acttacaaag gtgaacaagg cgcgaaagaa gcaggcaaaa
    59101 tgcgtgcaga aggcaaagat tacatcgtta aagatggcga tgtgatgaac ttcctgttca
    59161 acgtctaatt ccgctaatac taccctggcg gttaataacg agccattatg tgagttgcac
    59221 gtaatggctt ttttacgcct gctacgcgtc ttctttcaat tctgagttac caaaactcat
    59281 aatcagactc ctgacaatat taacaatgca gcttttatgt gataacaatc acagtgcatt
    59341 aaccgttcaa gagtttgtca tgaaaaaaac atttaccttc agcctgttag caattgtatt
    59401 tgcaagcctg ttgagtgctt gtgttcccca ccatcatcac cgccacagcg atggccctcg
    59461 tggtcctgcg ccctcaggta agccaatgcc accttcaaat ggtggaccaa atcacggcag
    59521 tcatggtcgc tattaattag caattcatag cgggaaagta catactctcc cgctatccac
    59581 ggcgcttaaa ctgccctgca tcaatgccat gttgcttcat tttccgccac agcgtagtgc
    59641 ggccgatccc aagtaaagcc gacatttcct gaatgcgacc gcctgtgacc tgggctgcgt
    59701 taataattgc ctctttttca acttccgcaa atgacagact ggtggaaagg cgggtggcgc
    59761 tgacatcatc tgtcgcctgc tcggtaaaca gatgttccgg caaatcactg acgcgaatgc
    59821 gcccattatc actactcaga gcaagattct cgatgacgct gtaaagttca aaatcgttgc
    59881 caggccatgc acaggaaacc aggcgagcga gggcatcgtc atcaattttc agccgcgtag
    59941 agaagcgttt ttcaaggctg cgtaatttgt tattcaccag cgccggaatg ctgccacgcc
    60001 gcatacgcag tggcgggatg gtaatttcaa atgcatgcag cgcgtaatac agctggcgac
    60061 taaaacgatt ttgttccacc agcattgcga ggtccgcggt cgttgtggca atcactttga
    60121 catcaatggg tattaaacgc cgcgcatcca gtcgcgtgat aaccccctgc ttgataacct
    60181 gaagcaaagc agactgtaac tccaccgcca gatattcaat cttttcaaga aacagcgtgc
    60241 cgccgtgcgc cagttccagc cgactcagac ggccattttc attatccgtg cgatcgccac
    60301 caataaattc ttccgccagc gccgcatcac catataattc acaattgacg gcgatataag
    60361 gccccgctgc acgctcgctt tcattatgaa ttgcctgact tagcagtgcc ttgcccacgc
    60421 cctcttctcc acaaagcagg acaggaaagc tactgcgtgc cgcctggcga ccaaaatgaa
    60481 tcaagcggcg ggtttgcgga tcgtcctgtg gcatatgagc gaaggtatgg ctgacttttc
    60541 ctaattgact ggtcattaac tgccgcatct gttccacagg atggagcaat aaaataaagc
    60601 tggttccctg cgtttctatt atcggtttaa gggttatcac cgcatcaata aactgatgct
    60661 gactttcaaa ggtggcttct acgtgtttga gcggatgtgc ctgttttatt gcttgttgca
    60721 atacggcggg taacgttaag agttcagtga ttgcccttcc ctgacttgcc gtcgcgtcaa
    60781 ggcgcaagac ccgcgccgcc tgggcattga taaattgcaa attaccctgc tcgtcccagc
    60841 taatcacgcc atcatccata ctttctaaca gggcattaag ttgatttaaa tgacggttag
    60901 tttcagcgag caaactgtcc gtcagcagta aatttccgac ctcgcgtgcg attgccaacg
    60961 tcaacggcaa atcagctgcg gtagtttgct caaccggaca cgccagcgct attgttcccg
    61021 tcaatcggcc cttgctgtca aacaacggcg ttgcgcaaaa ggcccagttc cagagtgcct
    61081 gtttgaaatg ttgatcggcc atcgttttca cggcctgacc agagatagcc gctaacgata
    61141 gcgcacaagt accaataatt ccctcggcgc aatacgtgcc gtcattgaat cccagtgtac
    61201 ttagttgctg caaggtttgc ggatcgccat tacggctgag aatgcaggcg gtttcatcga
    61261 ggataagcaa cgcacactct cgcggtgcca tatattccca ggcatcttcc agcgcagcct
    61321 gcccgagcgt cagcatcgct ttcttacgcc gataaataga agcaaatgtc acgccctggg
    61381 cctggtgtgg tacgttccat gtctcccgtt tcatcagctt attgcatctc tcccaggagg
    61441 ttgcaattaa gggagatatg ccccgaccat cgttgttaaa agcgccactc atatcccgca
    61501 tcccttatga caaaaggtgt tccggctcgc aattttaagt attaatacac gtaaaagttt
    61561 caatatggaa cattcattaa agcatatgtt tcactatgaa acctaatttg ggctaatttt
    61621 cttttccctt gcctgatgca caatggattc atcggcagtc agtggtcgcc gtgtcgttga
    61681 acatcatcca tgccctaccg taattgctgg agcaaaataa tgaaaaaatt gatcaatgat
    61741 gtgcaagacg tactggacga acaactggca ggactagcga aggcgcatcc atcgctgaca
    61801 ctgcatcagg atccggtgta tgtcacccga gctgatgccc ccgttgcagg gaaagtcgcc
    61861 ctgctgtcgg gtggcggcag cggacacgag ccgatgcact gtggctatat cggtcagggg
    61921 atgctttctg gggcctgtcc gggcgaaatt ttcacctcac cgacgcccga taaaatcttt
    61981 gaatgcgcca tgcaaattga tggcggcgaa ggtgtactgt tgattatcaa aaattacacc
    62041 ggcgatattc ttaacttcga aacagcaacc gagttactgc acgatagcgg cgtaaaagtg
    62101 accactgtgg tcattgatga cgacgttgca gtaaaagaca gtctttatac cgccgggcgg
    62161 cgcggcgttg ccaacaccgt attaattgaa aaactcgtag gtgcagcggc ggagcgtggc
    62221 gactcactgg acgcctgtgc ggaactgggg cgtaagttga ataatcaagg ccattcaata
    62281 ggtatcgctc tcggtgcctg taccgttcct gccgcgggca aaccttcttt taccctggcg
    62341 gataatgaga tggagtttgg cgtcggcatt catagtgagc caggtattga ccgccgcccc
    62401 ttctcttccc ttgatcaaac cgtcgatgaa atgttcgaca ccctgctgga aaatggctca
    62461 taccatcgca ctttgcgttt ctgggattat caacaaggca gctggcagga agaaccacaa
    62521 accaaacaac cgctccagtc tggcgatcgg gtgattgcgc tggttaacaa tcttggcgca
    62581 actccgcttt ctgagctgta cggcgtctat aaccgcctga ccacacgttg ccagcaagcg
    62641 ggattgacta tcgaacgtaa tttaattggc gcgtactgca cctcactgga tatgaccggt
    62701 ttctcaatca ccttactgaa agttgatgac gaaacgctgg cattctggga cgcccctgtc
    62761 cacaccccgg cccttaactg gggtaaataa ggagaaagca atgtcactga gcagaactca
    62821 aattgttaac tggctcactc gttgtggcga tattttcagc accgagagcg agtatcttac
    62881 tggactggat cgcgagattg gcgacgctga ccacgggcta aatatgaatc gaggctttag
    62941 caaagtggta gaaaaactcc ctgctatcgc agataaagat atcggtttca ttctcaagaa
    63001 taccggcatg acactacttt ccagcgtcgg aggtgccagt ggtccgctgt tcggtacctt
    63061 ctttatccgc gccgcacaga caacccaggc acggcaaagc ctgacgctgg aagagcttta
    63121 tcagatgttc cgcgatggcg cggacggcgt aatcagtcgc gggaaagccg aacctggcga
    63181 taaaaccatg tgtgatgtgt gggtgccggt ggtggaatcg ttacgtcagt cctgcgagca
    63241 aaatctccct gttccggcgg cgctcgacgc tgccagtagc atcgccgaat ccgctgcaca
    63301 aagtacgatt acgatgcaag cccgcaaagg ccgcgccagt tatctcggtg aacgcagtat
    63361 tggtcaccag gatcccggcg cgacctcggt gatgtttatg atgcaaatgt tggcgttagc
    63421 cgcaaaagag taaggaattg gtgatggtaa acctggtcat agtttcacat agcagccgac
    63481 tgggagaagg tgtcggtgaa ttagcccgtc agatgttaat gagtgataat tgtaaaatcg
    63541 ccattgccgc gggaattgac gatccacaaa atcccattgg taccgatgcc gtcaaagtga
    63601 tggaggccat cgaatctgtt gccgatgccg accatgtgct ggtcatgatg gatatgggta
    63661 gcgcattatt gagtgctgaa actgcgctgg aattgctggc tcccgagatc gccgcaaaag
    63721 tacgtttgtg tgctgcgccg ttggtcgaag gtacactggc agcaacggtc agcgcggcct
    63781 cgggggcgga tatcgacaaa gtgatctttg acgccatgca tgcgctggaa gccaaacgtg
    63841 aacaactggg tttaccgtcc tccgacactg aaatctctga cacatgtcct ccgtacgatg
    63901 aagaagcccg ttctctgtcg gtggtcataa aaaaccgtaa cggcctgcat gtacgtccgg
    63961 cctcccggct ggtttatacc ttatcgacat ttaatgccga tatgttgctg gagaaaaacg
    64021 gcaaatgcgt tacaccggag agtattaacc agattgcgtt actacaagtt cgctataacg
    64081 atacgctgcg cctgattgcg aaaggaccag aagctgaaga ggcactgatc gccttccgtc
    64141 agctggctga agataacttt ggtgaaacgg aggaagttgc tccacctact ctgcgtcccg
    64201 ttccgcctgt ttcgggtaaa gccttttatt atcaacctgt tttatgtacg gtacaggcaa
    64261 aatcaaccct gaccgtggaa gaagaacaag aacgattacg ccaggccatt gacttcacgt
    64321 tattagatct gatgacgtta acagcgaaag cagaaaccag cgggcttgac gatattgccg
    64381 caatcttttc tggtcaccat acactgttag atgatccgga actgctggcg gcggcaagcg
    64441 aactccttca gcatgaacat tgcacggcag aatatgcctg gcagcaagtt cttaaagaac
    64501 ttagccagca ataccaacag ctggatgatg aatatctaca ggctcgatat atcgatgtgg
    64561 acgatcttct gcatcgcacc ctggtccacc tgacccaaac gaaagaagaa ctcccgcagt
    64621 ttaactcgcc aactattcta ctggcggaga acatttatcc ctccacagta ctgcaactgg
    64681 atccagcggt tgtaaaaggt atctgcctta gcgccggaag tccgctatcc cacagtgccc
    64741 taatcgcccg tgaactggga attggctgga tttgccagca gggtgagaaa ctgtatgcga
    64801 tacaaccaga agaaacgcta acgctggacg ttaaaacgca acgtttcaac cgtcagggtt
    64861 aaaccaccgt tcggatggca tcattctgat gtcatccggc aatttactct gcactgatgt
    64921 tttttaacta cctgttatat ttttcaccaa attatgatga tttttcttcg ctaatcgcga
    64981 acccaacgtc gctggctgac taataaacag atgttatttt atcactctat ttcgccgtta
    65041 tttatccctt taattccctt ttctaaaatg tctgtcagtt cgcagaatgt gatttcgatc
    65101 atgcagctag tgcgatcctg aactaaggtt ttctgatact tgaataccgt tttattcggt
    65161 ttcgccaaag gagaatgact gatgaaatcc cccgcacctt ctcgcccgca aaaaatggcg
    65221 ttaattccag cctgcatctt tttgtgtttc gctgcgctat cggtgcaggc agaagaaaca
    65281 tcagtaacac cacaaccgcc tgatatttta ttagggccgc tgtttaatga tgtgcaaaac
    65341 gccaaacttt ttccggacca aaaaatcttt gccgatgccg tgccgaacag cgatccgctg
    65401 atgatccttg ctgattatcg gatgcagcaa aaccagagcg gatttgatct acgccatttc
    65461 gttaacgtca atttcaccct gccgaaagag ggcgagaaat atgttccgcc agaggggcaa
    65521 tcactgcgcg aacatattga cggactttgg ccggtattaa cgcgttctac cgaaaacacc
    65581 gaaaaatggg attctctatt accgctgcca aaaccttatg tcgtgccagg cggacgtttt
    65641 cgcgaggtat attactggga cagttacttc accatgttag gacttgccga aagcggtcac
    65701 tgggataaag tcgcggatat ggtggccaat tttgctcatg aaatagacac ttacggtcat
    65761 attcccaacg gcaaccgcag ttactattta agccgctcgc agccgccctt ctttgccctg
    65821 atggtagagt tactggcgca gcatgaaggc gacgccgcgt tgaaacaata cctgccgcaa
    65881 atgcaaaaag aatatgctta ctggatggac ggtgttgaaa acctgcaagc cggacaacag
    65941 gaaaaacgcg ttgtaaaact tcaggatggt acccttctca accgctactg ggacgatcgc
    66001 gatacgccac gaccagagtc atgggtggaa gatattgcca ctgccaaaag caatccgaat
    66061 cgacctgcca ctgaaattta ccgcgacctg cgctctgccg ctgcgtctgg ctgggatttc
    66121 agctcgcgct ggatggacaa tccgcagcag ttaaacacct tacgcaccac cagcatcgta
    66181 ccggtcgatc tgaacagctt gatgttcaaa atggaaaaaa tcctctcccg cgccagcaaa
    66241 gctgccggag ataacgcgat ggcaaaccag tacgaaacgc tggcaaatgc ccgtcaaaaa
    66301 gggatcgaaa aatacctgtg gaacgatcaa caaggctggt atgccgatta cgacctgaaa
    66361 agtcataaag tgcgcaatca gttaaccgcg gccgccctgt tcccgctgta cgtcaatgcg
    66421 gcagcgaaag atcgcgccag caaaatggcg acggcgacga aaacacatct gctgcaacca
    66481 ggcggcctga acaccacgtc ggtgaaaagt gggcaacaat gggatgcgcc aaacggctgg
    66541 gcaccgttgc agtgggtcgc gactgaagga ttacaaaact acgggcaaaa agaggtagcg
    66601 atggacatta gctggcactt cctgaccaat gttcagcaca cctatgaccg ggagaaaaag
    66661 ctggtggaaa aatatgatgt cagcaccacc ggaacagggg gcggcggtgg cgaatatcca
    66721 ttacaggatg gctttggctg gaccaatggc gtgacgctga aaatgctgga tttgatctgc
    66781 ccgaaagagc aaccgtgtga caatgttccg gcgacgcgtc cactcagtga atcaacaaca
    66841 cagccgctaa aacaaaaaga ggcggaacct acgccttaac tttactgtat atcgagcaat
    66901 gccattacgt gcataaaatt ggcattgctc tttcttcatc acgaaatacc aaataccact
    66961 ctttctacgg tctatgcctt tattttcttt tattagagaa gattttaatt ttattttatc
    67021 aacttgttaa tcattacacc tctatttata ttttccccca ctccccatcc tccctgacaa
    67081 ttttcttgtg gaacaaagtg ttatttaaga aacaagcaaa tcattctgtc tctttagaca
    67141 aattttgaca tcaaacatga tctttttcgc tatatgtgta agtatatagc ggttttaatt
    67201 atcaggatat tacttatgtc actaaccaga cggaggttta cacagattct tgcgtcgaca
    67261 ttgttcctgc atcatctgcc gtcctttgca caatcagtca aattctgggc ctcacggacg
    67321 cttcccgaag cccaaaacat tacacgcatc gtcagcgcag gcgcgcccgc cgatttacta
    67381 ttgctggctg tcgcgccaga aaaaatggtc ggtttttcct cttttgattt tgcccgtcag
    67441 gcattaattc cattgccaga gcacattcgc cagttcccca ggctgggacg actcgccggg
    67501 cgcgccagca cactctcgct ggaagggctt atggcgttac atcccgattt ggtcgttgat
    67561 tgcggcaata cggatgaaac ctggatctcc caggcacggc aggttagcga acagacacaa
    67621 ataccctggt tattgcttaa cgggaaactg gaacaatcag cagaacagtt aacaacgctt
    67681 ggcaaaacgt taggcgaaga gcaccgcgcc gcagaacaag ccaatctcgc cagccgcttc
    67741 gttggtgaag ctcaggcatt cgccacctca cccgccgcta atctcagctt ttatgctgcg
    67801 cgcggtcccc gagggctgga aacgggctta cagggttcgt tgcataccga ggcggcggaa
    67861 ttattaggtt tgcacaacgt cgcgcaaata gccgatcgcc acggtctgac acaggtttcc
    67921 atggaaaatc tcctgcgctg gcagccggat attattctgg ttcaggaggc cgttactgca
    67981 gattttattc gtcgtgatcc tctctggcag ggcgtgaaag cggttgcgga acaacgcatc
    68041 ttatttttaa gtggcctgcc ctttggctgg ctggatgccc cgccgggaat caaccgtctt
    68101 ctgggattac gcagacttca cgcctggctg gatcccgcca tcaatcgcca gtttaaaagt
    68161 gacatacagc attacgccca actgttctgg cattgttcac tcagtgacgc cgactatcaa
    68221 aaattggtgg cgagctaatg agaaccgtaa acggatgtat tttgctgctg gcagctatca
    68281 gtatcacttt tgccgccgta agtggggctt atcatctcga tatgcagcaa ctgctggcgc
    68341 tcattcttcg tcaagaaaat gttcctgtgc aggaacaaat tgtcttctgg caaatccgcg
    68401 tgccgcgtat tcttgctgcg ctgtttctgg gcgcggcgct ggcaggggcc ggaaccacct
    68461 atcagggaat gttccgtaat cccttagtgt caccggatat tttaggcgtc tccgccggtg
    68521 ccggacttgg tgcctgcgcg gcaatcctgt ggggattatc cattgtttac atccagctgt
    68581 acgcattttg cggcgggctg atggtggtcg cgggcgtctg gttgattacg cgtcgggtga
    68641 cccgccacga tccgatctta accctggtgc tggtaggcat tgcgttagga acgttgtgcg
    68701 gagcggggat atcgctaata aaaacgctgg ccgatcctta tacccagtta ccgtcaatta
    68761 ccttctggtt gctggggggg ctgtccaccg tgaccttacg cgatctctgc tatgctgcac
    68821 cgatcattct cataggctcc ttgcccctgt tttttctccg ctggcgcatg aatttactca
    68881 cgctttccga cgacgaagcc cgttcgctgg gactgaacgt gacacgtctg cgctttggat
    68941 tgattgtctg tgccacactc atcaccgcca gcacggtagc aatagcgggc ataattggtt
    69001 gggtcggcct ggtcgttccg cacattgccc gcttgttgac cggtcataac catcagcagc
    69061 tgttgcccat ggcaatgtgt actggcgcga ttctgctcct gcttaccgat acgctggcac
    69121 gcagcattgg aacaacagaa atcccacttg gcattctgac cgcttttgtt ggcgcaccgt
    69181 ttttcctgtt tttgttgtta cgcggaggtc gccagtgact ttgcttgccg tccgtcatgc
    69241 gtctctgggt tactcccgcc atccggtgct acgcgacgtg tccttcaccc tgtcgcaagg
    69301 aacaatttgt tgtctgttag gagccaatgg ttgcggcaaa accacactca tgcgctccat
    69361 tcttggcgtt ataccgttat taaaaggcga gatcttactg gatagcctcc ctgtgcagac
    69421 actcagccat cgccagcgcg cgcaggctat tgcctgggtg ccgcaggcgc acgacggcat
    69481 ttttgctttt agtgtgctgg atatggtgtt gatgggcctg gcccccacca taggcgcttt
    69541 ttccgttccc ggtaagcaag aacgcctgaa agcaacggag caactggaaa agctgggcat
    69601 tttgcatctg gcgacacgac gctggaatac gcttagcggc ggtgaacgtc agctggcttt
    69661 aatcgcccgt gcactcgtcc agcaaccgcg cttgttatta ctggatgagc ctgcctccag
    69721 cctcgatttc ggtcatcaga tccaacttct ggacacgctg gcgcagctca aaaataacgg
    69781 catgacaatg ttaatgtcga ctcatcatcc gctccatgca aacgccattg ccgacagcat
    69841 tatccaggta gagccagatg gacgagtaac acaaggctta ccgaccgaac agttaacaac
    69901 gaaaaaactg gctgccctgt accgggtttc tgccgaccag attcaccatc acctctctgc
    69961 aattaagcac taaggaaaaa tcatgctcat tgacgatatt gattttgcag acctttacct
    70021 tcagcaactc aggctggccc atcgcacaga aaaaacgccg gatcactggg atcaacgggc
    70081 ggaaaaaatg gcggaaaatt gcgcctcgcc gacggacagc tacctgcaac aactgacctc
    70141 aaaaattgac ctgcaaggcg cgcaaacctt attcgatatg ggctgcggac cgggtaccgt
    70201 atcgctggcg ctggcggaca aactcacgac gatttatggc gttgattaca gtcaggggat
    70261 gctgaacgtt gccgcccgtc gcgccgccgc gctaaaggca gacaatgtcc actggatcca
    70321 gcgcgcctgg gaagaggact ggagtgacct gccacgctgc gatattgccg tggcttcccg
    70381 ctccactctg gtggctgata tgcgccaggc aatgagcaaa ctgaataatc aggcacggct
    70441 tcgcgtctat acgactcatc tggtcagcac ctcgttcgtc tctccagcca ttcagcgggc
    70501 ggctggtcgg gaagtggtcg aattacccaa ctatatcttt gcgctcaacg tcctgtatca
    70561 gatgggaatt tatgctcacg ttgattttat ccgcggacaa aattgccaac aggataacag
    70621 cacctgggaa cgttttgagc agaacgtcag ctggagtctg ggcgcgctta acgacgatga
    70681 gcgtgagcgg ttatatcgct ggtatcaaca acaagatgct cgtgccctcg cccctgccag
    70741 ccgcgactgg gcgctcatct ggtgggatag cgtaccgcag gagacactgc gatgattttc
    70801 ctttctcagg cacaaatcga tgcgttactt ctggaagata tccagggcgg tgacctgacc
    70861 acgcgagcat taaatattgg acaccagcat ggctatatag agttttttct ccgtcagggc
    70921 ggctgcgtca gcggaatttc tgtcgcgtgt aagatgttaa ctacgctggg attaactatt
    70981 gatgacgcgg tcagcgacgg ttcacaagcg aacgcgggtc agcggctaat ccgtgcgcaa
    71041 ggtaatgccg cagcacttca tcagggatgg aaggcagtcc agaatgtgct ggagtggagt
    71101 tgcggtgttt ctgattatct cgctcaaatg ctggcgttac ttcgtgaacg ttaccctgat
    71161 ggcaatatcg cctgcacccg aaaagcaatt ccgggcaccc gcttactggc ctcgcaggca
    71221 attctggctg ccggaggact gattcatcgt gccggatgtg cggaaaccat attattgttt
    71281 gccaaccacc gccattttct tcatgacaat caggactggt caggcgcaat caatcagtta
    71341 cgtcgccacg cacccgagaa gaaaatagtt gttgaagccg acacgccgaa agaggcaatc
    71401 gccgcgttac gcgcgcaacc agacgtactt cagctcgaca aatttagtcc gcaacaggca
    71461 actgaaattg cgcaaatagc accgtcgctg gctccccact gcacgctggc tctgaccggc
    71521 ggaattaatc tgacaacact caaaaattac ctcgactgcg gcattcgcct ttttattacc
    71581 tccgcgcctt attacgcggc acctgctgac attaaagtca gtctgcaacc cgcagcctct
    71641 atttaattct ttgttaaacg gaatttattc tatgagactc aaaaaacgtt atctctgcac
    71701 agtattatca cttgccttta cccagcaggc cgtagcggct caggagagcg acacgctgac
    71761 cgtatggtcc agtccggtat catcaacgac gaccaccgtt ctcgatcaac ccaccatgaa
    71821 ggccctggat aaacagaatg tcgctcaggc attaagtgtc gtccccggcg tggtgctgca
    71881 aaagtcaggt agccgcaacg aaggaacagg tcaaagttcg tggctttgat agtcgtcagg
    71941 taccggtcta tttcgacggt gtgcccattt atgttcccta tgacggcaac ctcgatctgg
    72001 cgcggattct gaccaacaat ttgagggcag ttgaagtttc caaagggtat tcgtcgctgc
    72061 ttcaggggcc taatcagatg ggcggagcca ttaatatcac cacccagaag ccaacaaaac
    72121 ctctggaagc aaatctggga tatcgccagg gatggagccg tagccaagac aatgcctacg
    72181 atatgcatgc ttcatttgcc gccagcagcg atctggggta tttgcaagtc agcggtagcc
    72241 agctaaagca ggattttctc ggcctgccgc atggtgtaaa taatgatatt gcaggcaaac
    72301 acggcaagat gattaattca tcggctgatg ataaacgcgg cattgtgaag ctcggtttta
    72361 caccacgcga aaacgatgaa tacacattga cttacattaa gcaggatggt gaaaaagata
    72421 acccgccata cagtggaaat agtggtcaga aatcacgcta ctggcagtgg ccagagtatg
    72481 acaaagaaag tttttattat cagggaacga cccaactaaa cgatcgtttt accctgaaaa
    72541 gtcggctgta tcgcgacacc tttgaaaata cgctaatgat gtacaactcg ctggctgatt
    72601 tgaaaaataa aaaaggcagc tacagccatt attccgatta cagcgacggt gccgggttac
    72661 aactggcagc cgatgtgcgt gaaaacgatc tgctgtcgtt tgccgttaac tggaaagatg
    72721 acgtacaccg ggaaaaaggt gcgccgcacg ccgcttacga tcgctatgaa gatcgtacct
    72781 ggtcgctcgc cagtgaatat caatgggctg ctgccgataa tgtcgatgtc gtggctggaa
    72841 tcagctatga ctggcgcgat agcgtagaag cgaaaaaaca tgagaaagat ggcagtatca
    72901 cccattatga cgacaacaat cagtcagctt ttaactggca ggtgatgggg aaataccact
    72961 ttgccaatga agacacgctg gcgctttcgt actatgaccg cacacgcttt ccgacgctga
    73021 aagaacgcta taccacgtcc aaacctgcgt ataaccagat agcgattgtt aacccgcagc
    73081 tcaagccgga acgcgcgcgc ggggtggatt taacctggaa tggtgccttc acgcacgact
    73141 ggggatttga ggtcagcgtt tactataacc gggtgagtga tgccatcctc tcgcacaata
    73201 tcgatgccga taccattcaa aatcagaaca gcggcacggt ggattacagc ggtctggatg
    73261 ccggtattaa ggggaaaatc agcaatatac tggatgtagg attgagctac gccctgatcc
    73321 acgctgatgc caaacgcaaa gacatcggca agataaccga tctgccaacg cagacaatga
    73381 ccgcatggat gactctcaaa ccgtgggagc cgttaagcgt aacgctgtcg gaagaggcgc
    73441 gttcctccag ctacagcaac aatgacggtt cacaaaaagc cgccggtttt gcggtgactc
    73501 acattcgagc cgattacacc ttaggtcatg gcttcagcgt caatgcgtcg gtcaataacc
    73561 tgtttgatac caaatatgcc tacagtgaag ggtttattga agaaggtcgc aatttctggg
    73621 caggtatcga gtatacgttc tgatgtgaaa gcggatagcg gcatctgtat cgctatccgc
    73681 cttatgaaaa tcttattctc ttcgcaggag gcggaatacg cccagcacca ggatagcgcc
    73741 caccaccgcc accaggaagc tgtgcaaatt aaaaccgctg atggagccgc caatgccaaa
    73801 catggtcgcc agccagccgc cgaccaccgc accgacgatc ccaagaatac aggtcaggaa
    73861 aaatccacca ccatcacgcc ccggcatgat tagcttggcg ataataccgg ctatcaggcc
    73921 aaaaataatc caggcaataa ttcccatgtt caggccctcg tttcgatgaa tcggacgcaa
    73981 gaaatgctta cgtcaacgct ctaagtatag gcaagggcgg gatcgatgct gggcgtatgt
    74041 cggctgattt aagaacaaca tgtaaaaaat attcgcgcat ttgtattaag ttttgttaac
    74101 tgcgaccgat aaaccaaaga cagtttgtca gtcaggagtt tttccgcgtg agtcattacc
    74161 atgagcagtt cctgaaacaa aatccgttag ccgtcctggg cgtgttacgc gatttgaaca
    74221 aagccgcaat tcctttgcgt atcagctgga atggcgggca gctgatcagc aaaatactgg
    74281 caataactcc ggataaactg gtgctggatt tcggcagcca ggccgaagac aacatcgccg
    74341 tgctaaaggc gcagcacatt accattaccg ccgaaactca gggtgcgaaa gtcgagttta
    74401 ctgttgaaca actacagcag agtgaatact tgcagcttcc ggcatttatt accgtaccgc
    74461 ctcccacctt atggtttgta caacgacgcc gatatttccg catcaccgcc ccactccatc
    74521 cgccttattt ttgccagacc aaactggcgg ataacagtac gttacgtttc cgcctgtatg
    74581 atttgtcgtt aggcggcatg ggcgcattac tggaaacagc aaagcctgcc gaattacacg
    74641 aaggcatgcg cttcgctcag attgaagtca acatggggca atggggtgtt tttcactttg
    74701 acgcccagtt aatctccatc agcgagcgca aagtgattga tggcaaaaat gaaaccatca
    74761 ccactccccg tctgagcttc cgttttctta acgtcagccc gacggtggag cggcaattac
    74821 agcggattat tttctctctc gagcgagaag cccggaaaaa agcggacaat gtgcgcgact
    74881 gatttacatc gcgtccagtg cctgctcaag tttgtagata tagcgcggag cctgcggcgc
    74941 aggatgattt cgcgctacgt gatcgaggaa ctcatcagca tccagatcgt tgattttgct
    75001 gatcgccttt ttccgatctg acgagaaagt ccgtagcagc gcacctgccc cgttagcgta
    75061 tgacaccacc agcgcatatt gcagtacctt cggatcttca atgcctgcca gcgggccggt
    75121 ttccagaata ttcaggtaag ccgcccccat tgaaatatta cgctccggat ttttcagctc
    75181 gctggtcgtc ggctcaccac tccagcccat acgacgatat acatcacgtc cggaggttga
    75241 agcttttatc tgcatcaaac caatggcatt cgatttactc accgcgttcg gattaccacc
    75301 cgattcgata gcgataatcg ccgtgatcaa ttgtggatcg acgccccagg ctgcaccggc
    75361 tttctggctt attggcatcc actgcatcgc acgttgcacc ggaactttcg cgttccacgg
    75421 cgggttcgta tagtcatgct ttgatgaaca acccgctaat aacacaatca aaaaggcaaa
    75481 ccatctcaat ttcacttctt ctatccttat agcctggaat cgccgttgag gtgacaacgg
    75541 cacacctaag cggcatgata tacatttcgt ttctgttgtc agcaaggaat tgccatgtct
    75601 ctgtttcact taattgcccc atcgggttac tgcattaaac agcacgccgc gctgcgtggt
    75661 atccagcgcc ttaccgacgc ggggcatcag gtcaataacg tcgaggttat tgcccgtcgt
    75721 tgtgagcgtt ttgcgggtac ggaaacggag cgtctggaag atctcaattc tcttgccaga
    75781 ctgactaccc ccaacaccat cgtgctgtct gtacgcggcg gttacggtgc cagtcgttta
    75841 ctggcagata ttgactggca ggcactggtg gctcgccaac aacatgaccc gttactcatt
    75901 tgcggacata gcgattttac cgccattcag tgcggtcttc tggcgcaagg caatgtcatc
    75961 acctttagcg gccccatgct ggtagcaaac tttggcgcgg atgaactaaa tgcctttacc
    76021 gagcaccact tctggctggc gttacgcaat aaaacgttca ccattgaatg gcaaggggaa
    76081 ggtccgacat gtcagaccga aggcacgctg tggggaggca atcttgcgat gctgatttca
    76141 ctgattggta caccgtggat gccaaaaatt gagaacggta ttctggtgct ggaagatatt
    76201 aacgagcacc ctttccgcgt cgaacgtatg ctgttgcagc tctatcatgc tggaattttg
    76261 ccacgtcaga aggcgattat tctcggtagc tttagcggca gcacgcctaa tgattatgac
    76321 gcaggttaca acctggagtc agtgtacgcg tttttgcgtt cccgcctgtc gattccactc
    76381 attaccggcc tcgattttgg tcatgaacaa cgcacggtta cgctgccgtt gggcgcacat
    76441 gccattctga ataacaccca ggaaggcact cagttgacga ttagcggtca tcctgttctt
    76501 aaaatgtaaa aaaatcacgc ttcagggcta agtaaaacgc tgtctctgcc gctaatatgg
    76561 tagggtttat ataagagaca gcgtaatcag gagtaaccga ccgttggatg ccacaacaat
    76621 aattagcctt ttcatcttag gatccatcct tgtcaccagc agtattttac ttagttcatt
    76681 ttcttcccgt cttggcattc ccattctggt tatttttctg gcgatcggca tgctggcggg
    76741 ggttgatggc gtcggcggta tcccgtttga taattatccc ttcgcctaca tggtaagtaa
    76801 cctggcgctg gcgattattt tgctcgacgg cgggatgcgc actcaggcca gttcctttcg
    76861 tgtggcgtta ggaccggcac tgtcgctggc gacgctgggc gtgcttatca cctctggttt
    76921 aaccggcatg atggcggcgt ggctgtttaa tcttgatttg attgaaggat tattaatcgg
    76981 cgctatcgtc ggctccaccg atgctgcagc ggtcttttct ctgctgggtg gtaaggggct
    77041 taacgaacgt gttggctcga cgctggaaat tgaatccggc agtaatgatc caatggcggt
    77101 ctttctgacg attaccctga ttgtgatgat ccagcagcat gaaagcagtg tcagctggat
    77161 gttcgtggtc gatattctgc agcaatttgg cctcggaatt gtcatcgggc ttggcggtgg
    77221 ttatttactg ctgcaaatga ttaatcgcat cgccctgccc gccggattat atccattgct
    77281 ggcattaagc ggcggaattt taatttttgc tttaaccacg gcgctggaag gtagcggtat
    77341 tctggctgtt tatctgtgcg gttttctgct gggtaatcgg ccgattcgca accgctacgg
    77401 catcctgcaa aatttcgacg gcctcgcctg gctggcgcaa atcgccatgt tcctggtgct
    77461 ggggctattg gttaacccaa gcgatctgct gcctattgcc attccggcgc tcattttgtc
    77521 cgcatggatg attttcttcg cccgtcctct ttcggtattt gccggattgc taccgttccg
    77581 tggcttcaat ctgcgtgagc gcgtgtttat cagctgggta ggattacgcg gcgcggtgcc
    77641 gattattctg gctgtgttcc cgatgatggc ggggctggag aatgcgcgac tgttctttaa
    77701 tgtcgccttc tttgtggtcc tggtttcact gctattgcag ggaacatcac tctcgtgggc
    77761 ggcgaaaaaa gccaaagtgg tcgttccgcc agtgggacgt ccggtgtcac gcgttggcct
    77821 ggatattcat ccggaaaatc cgtgggagca gtttgtttat caattgagtg ccgataaatg
    77881 gtgcgtgggc gcggcactgc gtgatttgca tatgccaaaa gagacgcgta ttgcggcact
    77941 gtttcgtgat aaccagttgc ttcatcccac cggcagcacc cgactgcgcg aaggcgatgt
    78001 gttgtgtgta attggtcggg aacgcgatct cccggcgctc ggtaaactgt tcagccagtc
    78061 gccgccggtc gcgctggatc aacgcttctt tggtgacttc attctcgaag ccagcgccaa
    78121 atatgctgat gtggcgctga tatatggtct ggaagacgga cgagaatatc gcgataagca
    78181 gcaaacgctg ggtgaaatcg tccagcagtt gttaggcgca gcaccggttg tcggtgacca
    78241 ggtagagttt gccgggatga tctggacggt ggccgagaaa gaagataatg aagtgttgaa
    78301 gattggtgtt cgggtagcgg aggaagaagc cgaatcttaa gtttaatgtg ttttataacg
    78361 ctggatacga cgctggtcgc atccagcgat aacaagttac accgtcacaa ccgggacgcg
    78421 taacgccagc gcgcacatca actcatagcc taccgttccg gcagcggcgg cgacatcatc
    78481 aattttgatc tccttgcccc acagctcaac tggcgtacca atacccgctt gcgggcaagg
    78541 cgttaaatcg accgccagca tatccatcga gactgtcccc accgtcatgg tacgaacgcc
    78601 gtccactaaa acaggggtac cggtaggcgc gtggcgcgga taaccgtcgg cgtatcctgc
    78661 ggcgacaatg ccgattcgct gttcatcgcg cgcagtatag cgaccgccgt agcccacacg
    78721 ctcgcccgct tttagcgtct ggacaccaat aatctcactg cttagcgtca tcaccggacg
    78781 taatccggta ttggcgatat cacgccactg accggacggc gaagcgccat acaaaataat
    78841 accaggccga acccagtcaa aatgcgcttc ctggtgccac agagtcgccg ccgaattgga
    78901 caacgaacgc cgacactcca gcccttccgc cgcctgctca atacgcgcca tcgcgctgga
    78961 aattccatca ggatgttccg cttcggcaaa atgcgacatc agggtcattt cgccaacatt
    79021 cgccatcgcc cgcaactgct gccagacggt aagcacgcga tcgggctgga agcccaaccg
    79081 attcatccca ctgttcactt taagataaat atccaacggt gcttttagcc gcgcattttg
    79141 cagtgctttg agctgccagt tgctgtgtac gcaggtggtc aggcggtgct ggtcataaat
    79201 ctccagatcc tgagcatgga aaaatccttc cagcatcagg atcggtcctt tccagccgcg
    79261 ctcacgtaac gttattgcct cttccagatt aagcaatgca aagccatcgg tggccccgag
    79321 cgcgctccag atacgctcaa taccgtgccc gtaagcgttc gcttttacca ccgaccagac
    79381 gcgcgcgtac ggcgcggcct ggcggacaat ggacagattc tgttttaatg cctgcagatc
    79441 gaggctggcc tgtatcggac gggtcatctc gtttccttag ctgtgtgcgc catgtaaatg
    79501 gcccgggcgt gatggcgtaa atccacggct gtagcgcgct acgcttagat cttcatacgg
    79561 gatcgctggc gtgcgccccg agagcagatc gcttaacaac tgaccggaac cgcaagccat
    79621 cgtccagccg agcgtgccgt gaccggtatt cagccacaga tttttaaagc gtgtacgccc
    79681 gacaaccggc gtgccgtctg gcgtcatcgg gcgcaggcca gtccagaaag tcgcctgctc
    79741 aacatgaccg ccgcgtggat agagatcgcg aaccaccatc tccagcgttt cacgacgcgg
    79801 ttgcaacagc tcggtgttaa agccaacaat ttccgccatt ccgccaacac gaatgcggtt
    79861 atcgaaacgg gtaatggcga ttttgtaggt ttcatcaagg atagtagaca ccggcgcacc
    79921 atcttcctgc gcaatcggaa tagtcagcga gtagcctttc agcggataca ccggaatatc
    79981 aacaatgcct ttgagcatcg ccgtcgagta agaaccaaac gccatcacat acgcatcggc
    80041 cttaatcact tcatcaccac acttcacgcc gtagatttgc tcgccgtcgc aaagcagttg
    80101 gtcaacgggc gtattaaagt ggaatttaac ccccgtctgc tccgccatcc gcgccagatt
    80161 ctgggtaaac agctggcagt ctccggtttc atcattgggc agttgcaggc cgcccgtcag
    80221 tttgtgcgcc acttctgcca acgcgggctc cacttccgcc aggcggctgg attccagcag
    80281 ctgatacggt acgccagcat cttccagcac ggcaatatcg cgggtcgcgt tttcatactg
    80341 ttgttcggta cggaacagtt gcagcgtccc accctgacgc ccttcatact gaatattggt
    80401 ttcggcgcgc aatgctttca ggcaatcacg gctgtattcc gccagacgta ccatccgccc
    80461 tttgttttcc atgtagtggc tggtgtcgca gttacgtaac atttgccaca tccatttcaa
    80521 ctggaactgc gtaccgtcga gacgaaccgc cagcggcgca tggcgctgga acatccattt
    80581 aatcgctttt aaaggcacac ctggtgccgc ccacggtgca gcatatccgg gggagatttg
    80641 cccggcattc gcggcactgg tttccagggc ggcccccggc tcccgatcaa tgacggtgac
    80701 ctcatgtcct gcctgattta agtaccaggc gctggcaacg cctaccacac cacttcccag
    80761 tatgacaact cgcatagcca ctccgataac ggtagataca gtaaaagaat aataatctaa
    80821 ttacatcttg ataactcagt tgaaaatatt attcaacata cggctttttt atggtgagct
    80881 ggctcacatc tccctgactc tgcggcaata tggcgtcttt agtatctcct gctgaacgcc
    80941 ctttttatcc agcataaaat tccgttcaga agcggattag tggcactctg ataataagaa
    81001 atcgcaaaga aaaaaattct ccggcgacgc ggtttttaac aggtgttcta tgcttgaaat
    81061 gaggtgctcc acacagagag cgccgacaac aatgaggtgc gcgtatggcg acgatcgatt
    81121 ctatgaataa ggacaccaca cgtttgagcg atggacccga ctggacgttc gacctgctgg
    81181 atgtttatct ggcagagata gaccgggtgg cgaaactcta ccggctggat acctacccgc
    81241 accagattga agtgataacc tcagaacaga tgatggatgc ctactccagc gtcggcatgc
    81301 caattaacta tccgcactgg tcattcggta aaaagtttat cgagactgaa cggctgtata
    81361 agcacggtca gcaaggactg gcctatgaaa tcgtcattaa ctctaacccg tgtatcgctt
    81421 acctgatgga agagaacacc attaccatgc aagcgctggt gatggctcat gcctgctatg
    81481 ggcataactc tttcttcaaa aacaattact tattccgtag ctggaccgac gccagttcga
    81541 ttgtcgatta tctgattttt gcccgtaaat atattaccga gtgcgaagag cgttatggcg
    81601 ttgatgaagt agaacggctt ctggactcgt gccacgcgct gatgaactac ggcgtggacc
    81661 ggtacaaaca cccgcaaaaa atctcgctgc aagaagagaa agcccggcag aagagtcgcg
    81721 aagagtatct gcaaagtcag gtcaatatgc tctggcgtac cctgccgaag cgcgaggaag
    81781 agaaaacggt tgctgaagcg cgccgctatc cgtccgaacc acaagaaaac ctgctctatt
    81841 ttatggagaa aaatgcgcca ctgctggaat catggcagcg tgaaatcctg cgtattgtgc
    81901 gtaaggtgag ccagtatttt tatccgcaaa aacagactca ggtgatgaac gaaggctggg
    81961 cgaccttctg gcactacacc atccttaacc atctgtatga tgaagggaaa gtaacggaac
    82021 gttttatgct ggagtttttg cacagccaca ccaatgtggt cttccagccg ccttacaaca
    82081 gcccgtggta caacggcatt aacccatatg ctctcggatt cgccatgttc caggatatta
    82141 aacggatttg tcagtcacca acggaagaag acaaatactg gttcccggat atcgccggtt
    82201 ccgactggct ggaaacgctg catttcgcga tgcgtgattt caaagatgag agttttatca
    82261 gccagttcct gtcaccgaaa gtgatgcgtg atttccgctt cttcaccgtg ctggatgatg
    82321 atcggcataa ttatctggag atttccgcta ttcataatga agaaggttat cgggaaatcc
    82381 gtaaccggtt atcgtcgcaa tataacttaa gtaatctgga gccgaatatt cagatctgga
    82441 acgtggattt gcgcggcgac cgttcgctga cgctgcgtta cattccacat aatcgcgcac
    82501 cgctggatcg ggggcgcaaa gaagtgctga agcatgtgca tcgcctgtgg ggatttgatg
    82561 tgatgctgga acagcaaaac gaagacggca gcatcgagtt gctggaacgt tgcccgccga
    82621 gaatgggaaa tctgtaaata gtcctcctcg tttgaggagg actcattttt gccggatgcg
    82681 gcgtaaatgc cttatccggc ctacaaagta tcgcaaattc aatacattgc aaaaaaacac
    82741 gtaggcctga taagcgtagc gcatcaggca attatacgtt tgtcatccgt ctggaaggat
    82801 tatcgtccct gaatggctaa atcacccggc agatttttct gcatccggtg ccaaatctcg
    82861 ccactctcat gcccatagcg acgcactgtt tcgtatacct gatcgtgtgc gccttcactg
    82921 cacaacgccg acagtttgtg atagaagccc agcgccagac tgcgcgcttc cggattggcg
    82981 aaatagtgac gaccaatgcg cgtatacagc cctttcatcc cgttaaggat cagaccgtaa
    83041 atcgggttgc cggaagcaaa cgccaggccg cggaaaatgt tgtaatccag ctcggcaaag
    83101 gcgtcggcgt gatcggccac ttcattagcg gttgccagca cttcctgcgc tttatcggga
    83161 tgttgacgaa acgcggtgcg aataaaaata gttgaaatat tggtacgcac cgacagcaaa
    83221 ttatcaataa gctgcggcac actttcgtgg tccagtcgcg ccagtgtttc aaggatattt
    83281 aaaccggagg tttcccagaa attattcacc ttcgtcggct tgccgtgttg aatggtcaac
    83341 cagccatctc gtgccagacg ttgtaacact tcacgtaacg tagtacgcgt tacgccaatt
    83401 aattctgaaa gttcacgttc tgcgggcaaa atagtcccgg gagggaagcg gttattccag
    83461 atactttcaa taatgtactc ttccgcgaaa cccgccgggc tttgcgcctt aatgaccata
    83521 gtgagatttc cataacacag caaaacaaag ttggactcat cataccagag ggctaactac
    83581 gctgatagaa gacaaaaaga agaaaaaagg gatcaaggct tcattttctg catctctttc
    83641 cccttgacta tcaagacttg cctcactcct gtctgaccgt taagctttgc gtttaaacat
    83701 aaaaacatca ttgattattt ctaaggtaag ggaaaccatc atggagatct cctggggccg
    83761 cgcactatgg cgcaattttt tgggccagtc ccccgactgg tacaaactcg ccctcatcat
    83821 tttcttaatc gtaaatccgt taattttcct catcagccct ttcgtcgctg gctggttgct
    83881 ggtagcggaa tttattttca ctctggctat ggccctgaaa tgctacccgc tgctccccgg
    83941 tggtttgttg gctatcgaag cagtattcat cggcatgacc agcgcggaac acgtccgtga
    84001 agaggtggcg gcaaatcttg aagtcttgct gttactgatg tttatggtgg cgggtatcta
    84061 ttttatgaaa cagctgttgc tgttcatatt tacccgtttg ctgttaagta ttcgctccaa
    84121 aatgctgctg tcgctctctt tttgcgtggc ggcggcgttc ctctccgcgt tcctcgatgc
    84181 cttaaccgtc gtggcggtgg tgatcagcgt tgcagtcggt ttttatggta tttatcaccg
    84241 cgtagcctct tcccgtactg aagataccga cctgcaagac gatagtcata tcgacaagca
    84301 ttacaaagtg gttctggaac agttccgtgg ctttctgcgt agtctgatga tgcatgccgg
    84361 tgtcggcacc gcattaggcg gcgtgatgac catggtgggc gaaccacaaa acctgatcat
    84421 cgctaaagcg gctggctggc attttggcga tttcttcctg cgcatgtcgc cggtgaccgt
    84481 cccggttctg atttgtggcc tgttaacctg cctgctggta gagaagctgc gttggtttgg
    84541 ctacggtgaa acgctgccgg agaaagtccg cgaagtgctg caacagtttg acgatcaaag
    84601 ccgtctccag cgtacacgtc aggacaaaat tcgtctgatt gtgcaggcga ttatcggcgt
    84661 ctggctggtc accgcgctgg cactacattt ggcagaagtc ggtttgattg gtttgtcggt
    84721 cattattctg gcgacatcat tgaccggtgt taccgatgaa catgctatcg gtaaagcctt
    84781 caccgaatct ctgccattca ccgcactgtt gacggtgttt ttctcagtag tcgcggtgat
    84841 tatcgaccaa caactgtttt cgccgattat tcagtttgtg ttgcaggcat ctgaacatgc
    84901 tcagctgtcg ttgttctata ttttcaacgg tctgctgtca tccatttcgg ataacgtctt
    84961 cgtggggacg atttatatca acgaagcgaa agcggcaatg gaaagtggcg ctatcacgtt
    85021 gaagcaatat gagctgctgg cggtcgccat taataccggt accaatctgc cctccgtcgc
    85081 tacgccgaac ggtcaggctg cgttcctgtt cctgctgacc tctgcactcg cgccattgat
    85141 tcgtctctct tatggccgca tggtgtggat ggccctgcct tacaccctcg tcctgacact
    85201 cgtcgggctg ctctgcgtcg agtttacgct tgctcctgta accgaatggt ttatgcaaat
    85261 gggctggata gcaacgcttt gataacaact taccgggcat ataaatgccc ggcttgcctt
    85321 ttcgcccgat aattgtccaa ttgcgtccta tttttgctgc ctcctggtgg cgcagcgaat
    85381 gaattggttt aaactgcgca ctctatgcat attgcaggga aatgattatg ttgcgatttt
    85441 tgaaccaatg ttcacaaggc cggggcgcat ggctgttgat ggcgtttact gctctggcac
    85501 tggaactgac ggcgctgtgg ttccagcatg tgatgttact gaaaccttgc gtgctctgta
    85561 tttatgaacg ctgcgcgtta ttcggcgttc tgggtgctgc gctgattggc gcgatcgccc
    85621 cgaaaactcc gctgcgttat gtagcgatgg ttatctggtt gtatagtgcg ttccgcggtg
    85681 tgcagttaac ttacgagcac accatgcttc agctctatcc ttcgccgttt gcgacctgtg
    85741 attttatggc tcgtttcccg gaatggctgc cgctggataa atgggtgccg caagtgtttg
    85801 tcgcctctgg cgattgcgcc gagcgtcagt gggaattttt aggtctggaa atgccgcagt
    85861 ggctgctcgg tatttttatc gcttacctga ttgtcgcggt gctggtggtg atttcccagc
    85921 cgtttaaagc gaaaaaacgt gacctgttcg gtcgctaatc cattcggcgc tcctgcggga
    85981 gcgctttttc ctgccgctat gtttatttga cccgcagtaa atcagaactt cgcgttgtat
    86041 aacatggtga aagcatggca cgcttcatct gccattgttg ctggatcccc tgcccggcaa
    86101 aatagagtgt tcctctgccc tctttagcat tcagcgtatc cattaccgcc atcaattgct
    86161 cactcccggg gcgcggtgcg ttgtcatcga ataaattgag ctgcgcgact ccctgactga
    86221 agaaatcccc cagcatcacg cccgcttttt ggtagcgatg gcccgcttgc cagatggcat
    86281 ccagagatcg cgtagcggcg ttaatgatat ccctgctgtc ctgagtgggc gtcagcagtt
    86341 ttaccgatgc gctattcccg taataaggtt cattgaacgc aaatggtgac gtcttaataa
    86401 acgtagagat aaaccgacaa tattgatgct cgctgcgaag tttttccgcc gcccgggcag
    86461 cgtaactaca aatggcctgc cgcatcgacg gataatccgt gatgcgttca ccaaacgagc
    86521 gggaacagat aatttcctgc ttcgtcggtg caaactcttc cagttgcaaa cagggttcac
    86581 cgcgcagttc acgcaccgtt ctttcgagca cgacattaaa atgtttacgg ataaaccgga
    86641 tatctgaatc cgccaaatcg agaacggttt tgatccccat cgcgtccagt tttttgctga
    86701 tccgccgtcc aatcccccag acttcatcca cgggaagagc agacattaat ttacgctggc
    86761 gttccagatt tgataaatcc accaccccgc ccgtctgccg ctgccatttt tttgccgcat
    86821 gattggcaag cttagccagc gttttggtct gggcgatccc cacaccaaca gtaagatggg
    86881 tacgttgtag caccgttgcg cgaatttctc tgccaaaatc agtcagatcg cgacaattat
    86941 gcacacctgt cagatcgcag aatgcctcat caatactgta aatctcgacg cggggcgata
    87001 gttcttccag cgtcgacatc acccgattac tcatgtctgc gtaaagctca taattgctgc
    87061 taaagcaaac cacgccacag cgacgaaaca gatctttttg tttgaaccag ggatcgccca
    87121 ttttaacgcc cagcgccttt gcctcagcgt ttcgggcgat aacgcaaccg tcattattcg
    87181 atagcacaac caccggttta ccccataaat cagggcgaaa caccgtctca cagctggcat
    87241 aaaacgcgtt tacatcacag agggcaaaca tcagcgcatc gccttaacga cgtggatcac
    87301 cacaccaaag acatccagcg tatcttcgct actgatggta atgggcgagt acgcactgtt
    87361 cataggagta agctgtaccg tcgggcgtag ttgcaatttt ttcaccgtaa actcaccgtc
    87421 aacagcagcg atgacaatat caccatggct ggcggtaata gcgctatcga caatcagtaa
    87481 atcaccgtca ctaatcccac catcaatcat agaatcacca cttgctttga cgaaataagt
    87541 cgcgctggga tgctggatca acagttgatt cagatcgatg cgctgttcaa cgtaatctgc
    87601 ggccggtgaa ggaaagccac actgaacaag atcgctaaat agcggaaaag tcacaatttc
    87661 gcggagatcc gcaggcttga taaacaacat aataatctgc ctgaagttat actgttttta
    87721 tatacagtag tctgttcttg tcagcagatc aatactgatt caggctatca atatttgtcg
    87781 ctgcatagac tgctgatttt tcgttctctt atcctgtgct cacgcggcct tctggcgaca
    87841 gcgtccatcc agcagaaagg aaaaatatca cctggtttaa aatagaatcg aagcattcgc
    87901 ccagaaaggc attctcatta aggtactgga tgacggcatc aagaaactgt gttctattgc
    87961 tgcgggcgta gttgaaggga aactgattcc agaattgaag aacaagttaa aatctgcgca
    88021 gagtttcttt accaccctgt ctaacacggt taaacaagcg aataaagata tcgatgccgc
    88081 caaattgaaa ttaactaccg aaatagccgc catcggggag ataaaaacgg aaactgaaac
    88141 aaccagattc tacgttgatt atgatgattt aatgctttct ttactaaaag aagcggccaa
    88201 aaaaataatt aacacctgta atgagtatca gaaaagacac ggtaagaaga cactctttga
    88261 ggtatctgaa gtctgataag cgattattct ctctatgtac tcaaggtata aagtttatca
    88321 catttaaaaa tacagacgtt ttttaaagat tgagagttac ctgttgataa taaggtatcg
    88381 agtaactctc attatgacag agcgctttca aaaacagggc tacgaaatgg cgagcatcca
    88441 gacgtgaatt aagaaagccc ccatcaacat ctattaaatt tatcactgcg cccagtcagg
    88501 tttatttaag atatgatcct gccagtcaat cacttctgat tcttttactg cgatatgacg
    88561 cacagagata cgttcaccat gcattgccgc tttcgaacca gtaagtagcg gatgccacgc
    88621 aggtaaacct ttaccttccg ccagcaaacg ataagcgcag gtcattggca gccattcgaa
    88681 tgttggcaga ttttcacggg ttaatttaat gcagtcgggt tcaaactcga aacgacgttc
    88741 gtagttccga cattgacagg ttttaatatt gagctggcga caggcgacgt tagtgaagta
    88801 gatttcgtcg gtgtcttcat ccatcagttt atgcaggcaa cactgaccgc aaccatcaca
    88861 caacgactcc cattccgcat cgctcatttc gtccagggtt ttactttgcc agaaaggtac
    88921 atcgctcatc aggtgttccg ccattacgtt aaaacgcacc ttataaccag tctggcacag
    88981 cgatgcaagt tttgccgccg ctttcaggcg gcaaaaagta ttacaaaacg cgagttgtca
    89041 aagaatgccc atcgaaagtg actgtcagct catcaccgct ttgcaacggg ccgacaccat
    89101 caggcgtgcc tgtcagcaca acgtcaccgg ccttgagggt aaaaaacttg ctcatataag
    89161 cgatcagcgg aacgatttta tggatcatgt ccgcagtcgt accttgctgg cgttgctcgc
    89221 cgtttacgct caggcccagc gttgtatttt gcgggtcgcc ggtaaactcc gccgcgggaa
    89281 taaacccgga aagcggacaa gagttatcaa acgctttggc cttttcccac ggctgcccgg
    89341 ctttcttcat ttttccctga acatcacgca atgtcagatc gagcgccacg ccgtaaccgg
    89401 caatggcttt gcggacatgc tcttccgtag cctgacgcag cgtcgcgcca atcaacaccg
    89461 ccagttcgac ttcatgatga actgaaccga aatcggatgg gatcgccaat ggctgccgca
    89521 gatcgcacag tgccgtttct ggtttaataa acagcactgg ctcttcgggt actgcgctgc
    89581 ccatctcttt aatatgtttg gcatagttac tgccaacaca gactacttta ctcaccggat
    89641 aatccagcag cgcaccttgc cagttgtgat gttgatacat aacgttcccc tcaccttatc
    89701 gtggatatga aaacccgcgc cgacaaagat aatgccaccg ccggatatcg gttatttgtt
    89761 aatgtcgtct gttttttgcc ccatgacgga aagatgttgc ttcagcaaat cttcgggtgg
    89821 tggcggtaac tgcaaatagt aaccttgctc ggttaatgcc tgtttaacct tttcaatatc
    89881 ggcattaacc agtttcttcc gcccatccag cggaagaatc atcgctaact gaggctgacc
    89941 aaaacctttc atcagttcct caggaacacg cgaaaaatcg tctttttttt cgacatataa
    90001 ataggtctgg tcacgcttgc tgcttcgata aatcacacaa aacatacttt tactctttaa
    90061 gatgccaggt tttggcataa gctaacatca atgaaacccg cgccacaagc cagccagctg
    90121 tagatttcta cggtttgttc agtccatgcc ccctttataa gtaatccccc cggcaaaaat
    90181 gccgcgccgg gatttaaccg aacacaggaa agccactgga attctcccgt gaaggattgt
    90241 tatattataa cagttcatcg tactcattct gaacaggaga ctaccaatga aaattaaggg
    90301 catcagtaag gctgtattgt tgcttgcact gctaacctcc acgagctttg ctgcgggtaa
    90361 gaatgtcaat gttgagttca gaaaaggcca tagcagcgct cagtactccg gcgaaataaa
    90421 gggatacgat tacgatacat ataccttcta tgccaaaaaa ggtcagaaag tgcacgtgag
    90481 tatctctaat gaaggcgctg atacttacct cttcgggcca ggaatcgatg attctgttga
    90541 tttatccaga tattccccag aactcgatag tcacggacaa tactcactcc ctgcttctgg
    90601 caaatatgag ctgcgagtgc tgcaaacccg aaacgacgcc cgtaaaaaca aaactaaaaa
    90661 atataacgtc gatattcaaa ttaagtaaat atcaaccgac ggggactctc cccgtcataa
    90721 acgttaacgc cccactcctc tctgtcgctt aagaggttaa tgacgaatag aacataatgt
    90781 aaacaagaca tcactcacga cacataaata acatctttgt ggtggtttga ttgatttttc
    90841 cactcctctt accatccaca ccaaaataac gatcatcacg acataatttt tctttaacat
    90901 cgcaaaatac accgttagag aatgtaaatt cgctgggatc gtagccttgt aatgattgta
    90961 agttttcagc ggctttttcg ccgagatatt tccttgttaa ctctggggaa atgccatctg
    91021 aactcacaca aatatacggg tcgcaaacaa cgccacacgc aggagattga agcccgccag
    91081 cgtcatttcc agacgccatt gatgagaaca tcatccccac acctgacaat ccgatataaa
    91141 aaatacgcat catattcata gcacatcctg ataatccaac cgtacaacac ttatgctcat
    91201 ttcatcatca atccgtaatc aaaaccgctg acctggacga gttacgatga cctgacagaa
    91261 gtatcagacg tcttctgcgg tttctatgat agagcaattc tgtggcggat attaagataa
    91321 cgggtatctg ttctttaatg atgctgcaaa aaatggaaag ttaagaaagc ctggcgtggt
    91381 gaaaaaatac actcgcctca acactggata tttaaacagg tctgaagatg ctgtcacgat
    91441 tagggtgcgg aaaaaggaag cgtaaataag aacaatcaaa agtacgacgg caatgggttg
    91501 attgacaagg gtatttttta agctatgaat cagcgccatt tatcacagaa tagactttta
    91561 ctctgaataa atgggagggt gacttgcctc aatataatcc agactataac atgccttata
    91621 gtcttcggaa catcatcgcg cgctggcgat gattaatagc taattgagta aggccaggat
    91681 gtcaaacacg ccaatcgagc ttaaaggcag tagcttcact ttatctgtgg ttcatctgca
    91741 tgaggcagaa cctaaggtta tccatcaggc gctggaagac aaaatcgctc aggcccccgc
    91801 atttttaaaa catgcccccg ttgtactcaa cgtcagtgca ctggaagccc cggtaaactg
    91861 gtcagcgatg cataaggcgg tttcggcaac cggtttgcgg gttattggcg taagtggctg
    91921 caaagatgcg caacttaaag ccgaaattga aaagatgggg ctgcctatcc tgacggaagg
    91981 aaaggaaaaa gcgccgcgtc cagctcccgc accgcaggct ccagcgcaaa atacaacgcc
    92041 ggtcacaaaa acgcgtttaa ttgatacccc agtgcgttcc ggtcagcgta tttatgctcc
    92101 acaatgtgat ctgattgtta caagccacgt tagcgcaggg gccgaattga ttgccgatgg
    92161 taacattcat gtctatggca tgatgcgcgg tcgtgcgctg gcaggggcaa gtggtgaccg
    92221 ggaaacgcaa atattttgta cgaacctgat ggcggaactg gtgtccatcg caggtgaata
    92281 ctggctgagt gatcaaatcc cagcagaatt ttatggcaaa gcggcacgac tgcagttagt
    92341 cgaaaacgct ttgaccgttc aaccgttaaa ttgatccctt tttaacaagg aatttctatg
    92401 gcacgcatta ttgttgttac ttcgggcaaa gggggggttg gtaagacaac ctccagcgcg
    92461 gccatcgcca ctggtttggc ccagaaggga aagaaaactg tcgtgataga ttttgatatc
    92521 ggcctgcgta atctcgacct gattatgggt tgtgaacgcc gggtcgttta cgatttcgtc
    92581 aacgtcattc agggcgatgc aacgctaaac caggcgttaa ttaaagataa acgtactgaa
    92641 aatctctata ttcttccggc atcgcaaact cgcgataaag atgccctcac ccgtgaaggg
    92701 gtcgccaaag ttcttgatga tctgaaagcg atggattttg aatttatcgt ttgtgactcc
    92761 ccggcaggga ttgaaaccgg tgcgttaatg gcactctatt ttgcagacga agccattatt
    92821 accaccaacc cggaagtctc ctcagtacgc gactctgacc gtattttagg cattctggcg
    92881 tcgaaatcac gccgcgcaga aaatggcgaa gagccgatta aagagcacct actgttaacg
    92941 cgctataacc caggccgcgt aagcagaggt gacatgctga gcatggaaga tgtgctggag
    93001 atcctgcgca tcaaactcgt cggcgtgatc ccagaggatc aatcagtatt gcgcgcctct
    93061 aaccagggtg aaccggtcat tctcgacatt aacgccgatg cgggtaaagc ctacgcagat
    93121 accgtagaac gtctgttggg agaagaacgt cctttccgct tcattgaaga agagaagaaa
    93181 ggcttcctca aacgcttgtt cggaggataa gttatggcat tactcgattt ctttctctcg
    93241 cggaagaaaa acacagccaa cattgcaaaa gaacggttgc agattattgt tgctgaacgc
    93301 cgtcgcagcg atgcagaacc gcattatctg ccgcagttgc gtaaagatat tcttgaggtc
    93361 atttgtaaat atgtacaaat tgatcctgag atggtaaccg tacagcttga gcaaaaagat
    93421 ggcgatattt ctattcttga gctgaacgtg accttaccgg aagcagaaga gctgaaataa
    93481 gccccctgta aaagcgcatt tatcttcaag gcagagtcat ctctgccttg agtttctgtg
    93541 cacaataata ttgataaatc agattatctt acctttacaa cattgcgtta acagtcaatg
    93601 acacttcttg aataaaaata ttgtcaatca atgagttatt tatcatttga gattattctc
    93661 attcccgtaa aaaacataat tcctatcatc acagtcacgc tcattttgaa ttatctgaat
    93721 aatagcgagc gttctttagt taagcagatc ttaattgaag acgaaattat tgtgtgtgca
    93781 acttacttaa taccggacaa ctaagttgaa gaaaaaaata cttgccttcg ggcttatctc
    93841 agcattgttc tgctccacgc cagcgatggc ggatatgaat cgcaccacga aaggtgcatt
    93901 gctaggcgca ggcgttggtt tacttactgg caatggtgtt aatggcgtac tgaaaggtgc
    93961 agctgttggc gctggtgttg gtgcagtaac agaaaaaggc cgcgacggta aaaatgcgcg
    94021 taaaggtgca aaagtgggtg ccgccgttgg tgctgtcact ggcgttctga ccggcaatgg
    94081 cctcgaaggg gcgattaaag gtgccgtgat tggcggtacg ggtggtgcaa ttctgggtaa
    94141 aatgaaataa ggaatgatga tgaaaaaatt tgctttactg gcgggtctgt ttgttttcgc
    94201 accaatgacc tgggcgcagg actacaatat taagaacggt ttgccatcag aaacctatat
    94261 tacttgtgca gaagccaacg aaatggcaaa aactgacagc gcacaggttg cagaaattgt
    94321 tgcggtaatg ggtaatgcca gcgttgccag ccgtgattta aaaattgagc aatctccgga
    94381 actgagcgcc aaagtggtag aaaaactgaa tcaggtttgc gctaaagatc cgcaaatgct
    94441 gttgatcacg gcaattgatg acaccatgcg tgctatcggc aaaaaataat atttcatatg
    94501 ttaccgcgtc gatgaattcg acgcggtttt ttagctaacg ggcgggatca gaacttatat
    94561 ttcaggccca ccatcattgc ggtgtcattg tagccattat cacccagctg cacgcccaca
    94621 ttaccccaga cgctggccgc cggattcaga ttgccattta cgccatgttt catttccccc
    94681 agattacgcg ccccgtcttg ttcaactttc acaccgttta ttgagacagc aaaatcttta
    94741 ctgttatgga tcccgttcat ttcaacaaaa ggttcaaagc cgtcaccgtg cggaccgctg
    94801 tttttctcct gagtacgaat aaaggtgcgg aaaccaagtc ggatctgaat gttgttattt
    94861 gcgtcattct caatacgtga gccattggct tccgtgtgcg cattatgatc gacgcccatc
    94921 cagatggcct gtgccgaggt cgttctcaac tgtccgttgc tgtcacgcca ggcattatgc
    94981 ccgccaattt gacgtaacca aagttggctt gaacgcacct gttcagttac aggatcgata
    95041 tagcgcgttt cacctgcaca atcgttcaga cgcataacaa aaagagtgtt agcggctgcc
    95101 aggttggcga cgtaactgcc agcttcgggt cgcaggacgg gggtaggcgt ttctgttgat
    95161 tctgaagtga cattggaaaa aaataactct ctaccattgt atatttttgc aatgggggct
    95221 ttagtgccat ttgttgtaat agaacttttt tatatcaatg ttaccttgtc gaatagcaac
    95281 tgcataactt ccatcagcca cagtttcaag taccacataa tctaaattaa tatatgcttt
    95341 tttcccatta gcataagcac catagctatt aataccatta gttgttatgg aaccaccatt
    95401 catttttaaa tcagcacggc taataacttc gtaactgtta gaatcacaaa cttcatcatc
    95461 tacagcatcg acacatgttc gagactctcc aggacttgaa catgcaagaa ctcctactgc
    95521 atttttccca taggtaacaa tgttcgtttt atctgtggtt tcaagactgg tgttattttc
    95581 cacagtcaca gaatcattaa cctgacttaa aagtcccgct gaatatgccc cctcgtctcg
    95641 actgaattat caccattcat aacgatggtg ccaccaaatt cactgactac tgcgctggct
    95701 gttatccctc cattttcttt gtcagatacg ctttcagagc gtcttaatat tgctatcgct
    95761 tatatataat actttgtttt ggctatcaac agaaatacca taagatccat caccactagt
    95821 catcacactg gcattatgta tatatccagt tacaccgtca tccactttca cggcatggct
    95881 ttcgtcttaa gtgattagat actccccatt acttgtagaa gtattatttt tgatttcaat
    95941 cggtgattct tcatcggtta tataggaaat tatactataa ttatccaaca attgtgttgc
    96001 atatgcatta cccaccgcca gagcaataag tcctaaactg aaccagggga gttttttcag
    96061 tttcatagat taaccatcca ttcattggat taacaaacat tcctggtcaa ataccctatg
    96121 agttcagatt accaaaacgg aaggcaaaga taaaaacaaa agcacaagaa atttaaatag
    96181 ataaacagcg acaatattta aatgataaat aagccagcat tatcattttt agcttatttt
    96241 catgacattg atcactttgg ggtaactatt ttgtttaaca aataactctt cgacatcaaa
    96301 attatcaaca gttgccatgg aaattatttc actgatatat aaaacaactg tgataacacc
    96361 agggttgaca actatttgtg caataattcc tttccttcaa tgatccaggg gttcactgcc
    96421 agcaacccat tcagccgcat actggcagtg agaaactgaa agattagtgc tgatccctgt
    96481 ggctgacgag tctttccctt aatcgattaa ccagattgat cgccagccaa accagggcat
    96541 taagcaaatt tatcgtcaca agcacttctc ccagcccggt gctgggtttt gcaataagga
    96601 tgaccaggag agtgatcagc ataaatgcaa tggaaaagat gatatagaga gtgaaataat
    96661 ccagattatt actattgttc atgctactaa tggcaatcca gtcaaaactt tcacacaatt
    96721 atagcaaaca aaagaatgaa caatagtaac aagaggcaac gagctaatta gctgtctata
    96781 attacaccgc accatggcct tgctgatcaa ttttcactca accacaacct tcaccttcgg
    96841 tttagaaagg ataattttga ccatgtcgat atatgtaaca ggtttataga aataataacc
    96901 ctgctgaaat gtgatattat tttggttcag atagtcaaat tgttctttcg tttcgacacc
    96961 ttcagcgacg atactgatcg aaagtttacg cgccagatcc aatacacaat ccagaattcg
    97021 ggttgatgct gggtctgcgt tgacgcggcc aacgaaactg tgatcaattt tgatataatc
    97081 aatatgcagg tcatgcagat aagaaagccc tgagtaacca gtaccgaaat catccagcgc
    97141 gatgacaaaa ccattttcat gcagaatgtt caaccgctga accagacttt catcaacatt
    97201 taatggctca cgctcggtga cttccagaac aaggtttaaa tcgcggcggg taaaactgtc
    97261 acggtaattt aagcactcgt cgacaaacgt cggcgaaata atatgcgagg cgctaaaatt
    97321 aatcccaata tgaaaacctt ccggcagttt actcgcgata gcgttcatct gtctggcaac
    97381 ctgattcatc aggctttgcg taagcggaac gattaatccg catttttcag caagtggaat
    97441 aatgatatat atccaccgtg aggttgtttc cagcgggcta acacctcaac tccccgcaat
    97501 gtcccttccc gaccatttac cacaggttgg taaaaaggga cgatctcccc attatttata
    97561 gcccgtcgca ggatctcttc aggcgttgcg cttttattga aatacctatc aagcaggaag
    97621 gcagcggcgc aggcgattaa cagaataaat atcaaaatcc caaagccttt atcgataagt
    97681 ctgtagaagc taaagagacg ttcattcgct cgcgctgggg tctacagaga gcatgataga
    97741 gagcatcaca aatatccacg ccgaagataa aattttctcc aacttcatct agcctattaa
    97801 ttttttagca caatgtaggc agaattaaac aaattaggcg ggatatcagg cgtcaagaat
    97861 ggaaaacgag cgctctccat tcctgtttgc cctctgatat tgcggatata ttaagaaagc
    97921 ataacgcctg aaatgctcac tttgcatcag catagtgata cagctgatgt ttattcaaaa
    97981 accttactca agttttaaga gagcacggat tccctgtcat taataatgat atccgttagt
    98041 gtagtcttat gcatttcaat gtgctgcaag atttgcagat agagatcaga aagaataaca
    98101 ctggtctcat cttttaaacg caagcgtaaa gcctgagcaa taatatgact atcaacatat
    98161 ccatgtgtca tgaacacctc cctctcgaga tttttaaaca aagcacattg aaaaataaat
    98221 ctctctggga ttgaattatt catgtcggcc tcgcaggaat agtgatccaa taaaattatg
    98281 ctgtgtatag tctaaagcga cagacaaaaa taagatgaat gagttttcta taaacttata
    98341 ctttataatt aaaagttaca tatcatcagc tgtgtatcgc aacacgattt ccagtgtttt
    98401 tctaatcaca tcagctttga gaatatcact tgttgtttcc agggagtgta tcagacttaa
    98461 gataatattt ttattattaa cattatcgcc tgaaagcatt aaattggtca cagcctgccc
    98521 taacacagcg gactcttctt ctaacaaatt acctgaacta cgaaagtaac ttgataacgc
    98581 tttatctgat attgcattat gaatagtagt atcttcaagc atggtaacct ctcatcttac
    98641 ttatgaaatt ttaatgtatt ctgtttattt tcttaccagt gaacatactt ttattgtcag
    98701 ctatcgctgt tcttcggttt ggccctcctg tatcactgtt gctaaaatca cgaattatgc
    98761 tgtcaattaa atctcgtcgg aaagtatttt cctcagccga tttcattacg agtaattgct
    98821 taataagagt ctgcaacgaa aaatttatct tcatgcgcag tatctggagc accgcctggc
    98881 cagtaatttc atattttata ctttcattat cagatgtctt cataacgact tcatagctaa
    98941 cctgttaaat aatcaggctg ttgcattatt caaaaagcaa cccaattagt gcattataat
    99001 gtttctgttc ctcttcccct gtcgcttgtt ccagccggca aagaagtttg gcacaaagcg
    99061 actttcgact cagattacgt ccatctttca aaatctcagt cacaatttcc ccgagatttt
    99121 cctgctgagt aggcaagtta gcttttgcaa agtaacgagt gattgctcct gcagaatctg
    99181 aagtcactga attttgatgc atgctacgcc tctgttaaaa atgttaatca atgttaagtg
    99241 aggtaacaat agcaaacttg tacgaaatat gtgtacagaa tgataaatta atttttcgca
    99301 attagtgcta attcatataa aacaatgcat tacgataaaa catattgcat catttaaatg
    99361 acaacctata caggtttttt cgccaaaccg tcttcaacct tttccacttt ttatcaatgt
    99421 aaagaaacgg ttatgttttg gtattaaaag aaagacacaa gaagatatgt ctgttaccgt
    99481 cttactctca cctcgcccat taccctggat tgttaatgct taccaccctt atttatcgta
    99541 gccatatacg tggcgacgaa cctgtcaaaa aaattgaaga aatggtttcg atagcaaatc
    99601 gcaggaacat gcggtctgac gtaacaggga tcttactgtt taatggttct cattttttcc
    99661 agcttctgga aggtccggaa gaacaggttc aaatgatata tcgggccata tgccaggatc
    99721 cacggcacta taatattgtt gaactgttgt gcgattacgc gcctgctcgc cgttttggca
    99781 aagcaggaat ggaattattt gatttacgcc tgcacgagcg agatgacgtt ttacaggccg
    99841 tattcgacaa aggcacatca aaatttcagc taacttatga tgacagagcg ctacaatttt
    99901 ttcgtacttt tgtccttgca actgaacaat caacctattt cgagattcct gctgaagact
    99961 cctggctttt tatcgctgac ggatctgata aagaacttga ttcttgtacc ctttcatcaa
   100021 atataaacga ccactttgcc tttcatccta ttgtcgaccc tttatcgcgg cggataatcg
   100081 cttttgaagc cattgtgcaa aaaaatgaag acagcccgtc agctatagcg gttgggcagc
   100141 gtaaagacga ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca tttgcgatgg
   100201 cacacgcact taagctcggt gataaaatga tttcaatcaa tctattacct atgaccctgg
   100261 ttaacaaacc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat gctctggtac
   100321 ctgaacaaat catcgttgaa tttactgaaa gtgaagtcat atctcggttt gatgagtttg
   100381 ccgaagcggt taaatcgcta aaggcggccg gtatcagtgt agcaattgat cattttggcg
   100441 caggttttgc tggtttgtta ctcctgtcac gcttccagcc tgacagaatt aaaatcagtc
   100501 aggaattgat taccaatgtt cataaaagcg gaccacggca ggcaattatt caggcgatca
   100561 tgaaatgctg tacatcactt gaaattcaag tctctgctat gggcgtggca acaccagaag
   100621 agtggatgtg gcttgaatct gcaggaattg agatgtttca gggagatctg ttttccaaag
   100681 ctaaattgaa tggtatccct tcagttgcgt ggccggagaa aaaataattt tcagcacatt
   100741 atttcgcatg atttctggaa atcatcgtga aaatgaatac gttatgtttt aatttaatcc
   100801 acattaaaaa taatgtttca tccaatttag ctgaaatctt atttatactt tgtacaattt
   100861 tagcatatat tgtacaaagt attattgggt cgtgtagagg cgacggagat ttatgatcgc
   100921 aaggaggaat tgtggcttat tacagcattg gtgatgttgc tgaacgttgc gggattaatc
   100981 ctgtcactct ccgggcctgg caacgccgct acggtttgtt aaaaccacaa cgcagtgaag
   101041 gcggacaccg actctttgat gaagaagaca tacaacgcat cgaagagatt aagcgttgga
   101101 taagtaatgg cgttcctgta ggcaaagtta aagcattact ggaaactacc agccaggata
   101161 cgcaagatga ctggaaccgc ctgcaagaag agatgatgtc aattcttcgt atggctaatc
   101221 cagccaaact acgcgcgcga attatttcac tgggtcgaga gtacccagta gatcaattga
   101281 ttaatcatgt ttaccttcct gttcgccagc gtctcgtgct tgatcacaac acctcccgca
   101341 ttatgagcag tatgtttgac ggcgcattaa ttgaatacgc agcggcctca cttttcgaaa
   101401 tgcgccgtaa gcccggtaaa gaggccattc tgatggcgtg gaatgttgaa gagagagcac
   101461 gattgtggct ggaagcatgg cgtttatcat tgtcaggatg gcacatttct gttcttgctg
   101521 atcccattga atcgccgcgc ccggaactgt tcccaacgca aacattgatt gtctggacag
   101581 gcatggcacc aacgagaagg caaaacgaac ttttacaaca ctggggtgag caaggttata
   101641 aagtcatctt tcatgccccc taaattcacc tttatcccgc cccttagaca gaatgttata
   101701 atctgaaccc acaccagcgt ttgtagcgga gctggtccgt tgcaaaatgc ccaccgggag
   101761 aattcaggta atcccccttt cacactttgt acatatcaca gccaaaggac agaccagatt
   101821 gaagtggtaa aaccgatcgt gcacactacg cagtctcaca cctaacaaga ggttattatc
   101881 ctgatggatc aggtcgttgt ttttcaaaag atgtttgagc aagtgagaaa agagcaaaac
   101941 ttctcctggt tttattcaga attaaaacat caccgtattg cacattacat ttattatctg
   102001 gctacggata acatcagaat tattactcac gatgacacgg ttttgttatt aagaggaacc
   102061 agaaacctgt taaaagttag tacgaccaag aacccagcta aaataaaaga ggccgcattg
   102121 cttcatattc gcggaaaatc tacatttcgg gaatactgtt caacactggc aggcgctggc
   102181 gttttccggt gggttactga tgttaatcat aacaaacgca gttactacgc cattgataat
   102241 acgcttttat atattgaaga tgtagaaaat aataaaccat taatctagct aaagttggga
   102301 gcttaaaaaa tgcttcataa ttcagtaagg cattagcata atggaaataa aagtgcagag
   102361 actatcccta tggatgatta atactgtctt tttattgctc cccataaata atcaccagac
   102421 caatacgatc aaattgatat ttgaaatgtg ttcacttgac ttttgatacg ttattttata
   102481 acggttaaca tatttataaa aacaacgggc gtgttatacg cccgtttcaa tatttaacac
   102541 atgtagagat tacatgttct tgatgatcgc gtcaccaaac tctgaacatt tcagcagttt
   102601 agcgccttcc atcagacgtt cgaagtcata ggttacggtc ttcgcgttga
//
LOCUS       DAZFFE010000003       102503 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFFE010000003 DAZFFE010000000
VERSION     DAZFFE010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 102503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 102503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..102503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.003"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            323..415
                     /locus_tag="ACHJ69_000226"
                     /pseudo
     CDS             323..415
                     /locus_tag="ACHJ69_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            418..480
                     /locus_tag="ACHJ69_000227"
                     /pseudo
     CDS             418..480
                     /locus_tag="ACHJ69_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(624..1000)
                     /gene="rnpB"
                     /locus_tag="ACHJ69_000228"
     ncRNA           complement(624..1000)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHJ69_000228"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1033..2178)
                     /gene="garK"
                     /locus_tag="ACHJ69_000229"
     CDS             complement(1033..2178)
                     /gene="garK"
                     /locus_tag="ACHJ69_000229"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO5013302.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDSGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEALRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2275..3165)
                     /gene="garR"
                     /locus_tag="ACHJ69_000230"
     CDS             complement(2275..3165)
                     /gene="garR"
                     /locus_tag="ACHJ69_000230"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO5013303.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3195..3965)
                     /gene="garL"
                     /locus_tag="ACHJ69_000231"
     CDS             complement(3195..3965)
                     /gene="garL"
                     /locus_tag="ACHJ69_000231"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO5013304.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3981..5315)
                     /gene="garP"
                     /locus_tag="ACHJ69_000232"
     CDS             complement(3981..5315)
                     /gene="garP"
                     /locus_tag="ACHJ69_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO5013305.1"
                     /translation="MMLDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5690..7261
                     /gene="garD"
                     /locus_tag="ACHJ69_000233"
     CDS             5690..7261
                     /gene="garD"
                     /locus_tag="ACHJ69_000233"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO5013306.1"
                     /translation="MANIKIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAECHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7410..7745
                     /gene="prlF"
                     /locus_tag="ACHJ69_000234"
     CDS             7410..7745
                     /gene="prlF"
                     /locus_tag="ACHJ69_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO5013307.1"
                     /translation="MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7745..8209
                     /gene="yhaV"
                     /locus_tag="ACHJ69_000235"
     CDS             7745..8209
                     /gene="yhaV"
                     /locus_tag="ACHJ69_000235"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO5013308.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEYITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(8264..9073)
                     /gene="agaR"
                     /locus_tag="ACHJ69_000236"
     CDS             complement(8264..9073)
                     /gene="agaR"
                     /locus_tag="ACHJ69_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO5013309.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVVERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            9322..10602
                     /gene="kbaZ"
                     /locus_tag="ACHJ69_000237"
     CDS             9322..10602
                     /gene="kbaZ"
                     /locus_tag="ACHJ69_000237"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO5013310.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLETAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10625..11098
                     /gene="agaV"
                     /locus_tag="ACHJ69_000238"
     CDS             10625..11098
                     /gene="agaV"
                     /locus_tag="ACHJ69_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO5013311.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDLVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            11109..11888
                     /gene="agaW"
                     /locus_tag="ACHJ69_000239"
     CDS             11109..11888
                     /gene="agaW"
                     /locus_tag="ACHJ69_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO5013312.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11878..12756
                     /gene="agaE"
                     /locus_tag="ACHJ69_000240"
     CDS             11878..12756
                     /gene="agaE"
                     /locus_tag="ACHJ69_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO5013313.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12774..13208
                     /gene="agaF"
                     /locus_tag="ACHJ69_000241"
     CDS             12774..13208
                     /gene="agaF"
                     /locus_tag="ACHJ69_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO5013314.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTAQL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            13205..14338
                     /gene="nagA"
                     /locus_tag="ACHJ69_000242"
     CDS             13205..14338
                     /gene="nagA"
                     /locus_tag="ACHJ69_000242"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO5013315.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDALDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIALRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGHYTLCGEEVQMRGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            14688..15842
                     /gene="agaS"
                     /locus_tag="ACHJ69_000243"
     CDS             14688..15842
                     /gene="agaS"
                     /locus_tag="ACHJ69_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO5013316.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLQIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVKLANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPEMINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESNDVIAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15855..16715
                     /gene="kbaY"
                     /locus_tag="ACHJ69_000244"
     CDS             15855..16715
                     /gene="kbaY"
                     /locus_tag="ACHJ69_000244"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO5013317.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16882..17358
                     /gene="agaB"
                     /locus_tag="ACHJ69_000245"
     CDS             16882..17358
                     /gene="agaB"
                     /locus_tag="ACHJ69_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO5013318.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDA
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            17397..18200
                     /gene="agaC"
                     /locus_tag="ACHJ69_000246"
     CDS             17397..18200
                     /gene="agaC"
                     /locus_tag="ACHJ69_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO5013319.1"
                     /translation="MHEITLLQGLSLAALVFFLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            18190..18981
                     /gene="agaD"
                     /locus_tag="ACHJ69_000247"
     CDS             18190..18981
                     /gene="agaD"
                     /locus_tag="ACHJ69_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO5013320.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18982..19749
                     /gene="agaI"
                     /locus_tag="ACHJ69_000248"
     CDS             18982..19749
                     /gene="agaI"
                     /locus_tag="ACHJ69_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO5013321.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     RKPDAVICLATGATPLLTYHYLVEQIHQQQVNVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDAGTQQHEMLKTAGRPVTRGITLGLKDILNTREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHNHFTCVIDEMCRR"
     gene            complement(19799..20662)
                     /gene="rsmI"
                     /locus_tag="ACHJ69_000249"
     CDS             complement(19799..20662)
                     /gene="rsmI"
                     /locus_tag="ACHJ69_000249"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO5013322.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            20727..22763
                     /gene="lpoA"
                     /locus_tag="ACHJ69_000250"
     CDS             20727..22763
                     /gene="lpoA"
                     /locus_tag="ACHJ69_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO5013323.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRIWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVVAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     SVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGPVLQQKFGSTSELRAGVNGGSGIALTGSPITPTETT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            22721..23116
                     /gene="yraN"
                     /locus_tag="ACHJ69_000251"
     CDS             22721..23116
                     /gene="yraN"
                     /locus_tag="ACHJ69_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO5013324.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTIFIEVRYRRSALYGGSAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            23136..23726
                     /gene="diaA"
                     /locus_tag="ACHJ69_000252"
     CDS             23136..23726
                     /gene="diaA"
                     /locus_tag="ACHJ69_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO5013325.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            23736..24311
                     /gene="dolP"
                     /locus_tag="ACHJ69_000253"
     CDS             23736..24311
                     /gene="dolP"
                     /locus_tag="ACHJ69_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO5013326.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(24424..25464)
                     /gene="yraQ"
                     /locus_tag="ACHJ69_000254"
     CDS             complement(24424..25464)
                     /gene="yraQ"
                     /locus_tag="ACHJ69_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO5013327.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGGILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPESQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLV"
     gene            complement(25537..26172)
                     /gene="yraR"
                     /locus_tag="ACHJ69_000255"
     CDS             complement(25537..26172)
                     /gene="yraR"
                     /locus_tag="ACHJ69_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO5013328.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            26300..26818
                     /gene="yhbO"
                     /locus_tag="ACHJ69_000256"
     CDS             26300..26818
                     /gene="yhbO"
                     /locus_tag="ACHJ69_000256"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO5013329.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIVIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(26798..27241)
                     /gene="yhbP"
                     /locus_tag="ACHJ69_000257"
     CDS             complement(26798..27241)
                     /gene="yhbP"
                     /locus_tag="ACHJ69_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO5013330.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAIAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            27292..27594
                     /gene="yhbQ"
                     /locus_tag="ACHJ69_000258"
     CDS             27292..27594
                     /gene="yhbQ"
                     /locus_tag="ACHJ69_000258"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO5013331.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAVFAELLNSLQTPEIKSD"
     gene            complement(27581..28084)
                     /gene="yhbS"
                     /locus_tag="ACHJ69_000259"
     CDS             complement(27581..28084)
                     /gene="yhbS"
                     /locus_tag="ACHJ69_000259"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5013332.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(28078..28602)
                     /gene="ubiT"
                     /locus_tag="ACHJ69_000260"
     CDS             complement(28078..28602)
                     /gene="ubiT"
                     /locus_tag="ACHJ69_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO5013333.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            28811..29806
                     /gene="ubiU"
                     /locus_tag="ACHJ69_000261"
     CDS             28811..29806
                     /gene="ubiU"
                     /locus_tag="ACHJ69_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO5013334.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            29815..30693
                     /gene="ubiV"
                     /locus_tag="ACHJ69_000262"
     CDS             29815..30693
                     /gene="ubiV"
                     /locus_tag="ACHJ69_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO5013335.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQDTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            30774..31781
                     /gene="yhbW"
                     /locus_tag="ACHJ69_000263"
     CDS             30774..31781
                     /gene="yhbW"
                     /locus_tag="ACHJ69_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFO5013336.1"
                     /translation="MTDKTIPFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(31899..33143)
                     /gene="mtr"
                     /locus_tag="ACHJ69_000264"
     CDS             complement(31899..33143)
                     /gene="mtr"
                     /locus_tag="ACHJ69_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO5013337.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIMLIL
                     VFGVGNALVHILSSFNVLPVYQ"
     gene            complement(33297..35186)
                     /gene="deaD"
                     /locus_tag="ACHJ69_000265"
     CDS             complement(33297..35186)
                     /gene="deaD"
                     /locus_tag="ACHJ69_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO5013338.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRDTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(35179..35259)
                     /gene="yrbN"
                     /locus_tag="ACHJ69_000266"
     CDS             complement(35179..35259)
                     /gene="yrbN"
                     /locus_tag="ACHJ69_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO5013339.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(35366..36250)
                     /gene="nlpI"
                     /locus_tag="ACHJ69_000267"
     CDS             complement(35366..36250)
                     /gene="nlpI"
                     /locus_tag="ACHJ69_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO5013340.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARIEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(36359..38494)
                     /gene="pnp"
                     /locus_tag="ACHJ69_000268"
     CDS             complement(36359..38494)
                     /gene="pnp"
                     /locus_tag="ACHJ69_000268"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO5013341.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(38741..39010)
                     /gene="rpsO"
                     /locus_tag="ACHJ69_000269"
     CDS             complement(38741..39010)
                     /gene="rpsO"
                     /locus_tag="ACHJ69_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO5013342.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(39159..40103)
                     /gene="truB"
                     /locus_tag="ACHJ69_000270"
     CDS             complement(39159..40103)
                     /gene="truB"
                     /locus_tag="ACHJ69_000270"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO5013343.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(40103..40504)
                     /gene="rbfA"
                     /locus_tag="ACHJ69_000271"
     CDS             complement(40103..40504)
                     /gene="rbfA"
                     /locus_tag="ACHJ69_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO5013344.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(40668..43340)
                     /gene="infB"
                     /locus_tag="ACHJ69_000272"
     CDS             complement(40668..43340)
                     /gene="infB"
                     /locus_tag="ACHJ69_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO5013345.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAGVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(43365..44852)
                     /gene="nusA"
                     /locus_tag="ACHJ69_000273"
     CDS             complement(43365..44852)
                     /gene="nusA"
                     /locus_tag="ACHJ69_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO5013346.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(44880..45332)
                     /gene="rimP"
                     /locus_tag="ACHJ69_000274"
     CDS             complement(44880..45332)
                     /gene="rimP"
                     /locus_tag="ACHJ69_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO5013347.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(45539..45615)
                     /locus_tag="ACHJ69_000275"
     tRNA            complement(45539..45615)
                     /locus_tag="ACHJ69_000275"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45579..45581),aa:Met,seq:cat)
     gene            45963..47306
                     /gene="argG"
                     /locus_tag="ACHJ69_000276"
     CDS             45963..47306
                     /gene="argG"
                     /locus_tag="ACHJ69_000276"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO5013348.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(47314..48939)
                     /gene="yhbX"
                     /locus_tag="ACHJ69_000277"
     CDS             complement(47314..48939)
                     /gene="yhbX"
                     /locus_tag="ACHJ69_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO5013349.1"
                     /translation="MTVFNKFVKSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRILSLPLRLLIAVPFVLLTAADMSISLYSWCIFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFTFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKEAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMFLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDCRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVLLRKEFTEDNQGNPTPEGQG"
     gene            complement(49396..49482)
                     /locus_tag="ACHJ69_000278"
     tRNA            complement(49396..49482)
                     /locus_tag="ACHJ69_000278"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49446..49448),aa:Leu,seq:gag)
     gene            complement(49497..49829)
                     /gene="secG"
                     /locus_tag="ACHJ69_000279"
     CDS             complement(49497..49829)
                     /gene="secG"
                     /locus_tag="ACHJ69_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO5013350.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(50057..51394)
                     /gene="glmM"
                     /locus_tag="ACHJ69_000280"
     CDS             complement(50057..51394)
                     /gene="glmM"
                     /locus_tag="ACHJ69_000280"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO5013351.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(51387..52235)
                     /gene="folP"
                     /locus_tag="ACHJ69_000281"
     CDS             complement(51387..52235)
                     /gene="folP"
                     /locus_tag="ACHJ69_000281"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO5013352.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(52325..54259)
                     /gene="ftsH"
                     /locus_tag="ACHJ69_000282"
     CDS             complement(52325..54259)
                     /gene="ftsH"
                     /locus_tag="ACHJ69_000282"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO5013353.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(54359..54988)
                     /gene="rlmE"
                     /locus_tag="ACHJ69_000283"
     CDS             complement(54359..54988)
                     /gene="rlmE"
                     /locus_tag="ACHJ69_000283"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO5013354.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            55114..55407
                     /gene="yhbY"
                     /locus_tag="ACHJ69_000284"
     CDS             55114..55407
                     /gene="yhbY"
                     /locus_tag="ACHJ69_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO5013355.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(55563..56039)
                     /gene="greA"
                     /locus_tag="ACHJ69_000285"
     CDS             complement(55563..56039)
                     /gene="greA"
                     /locus_tag="ACHJ69_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO5013356.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            56286..57719
                     /gene="dacB"
                     /locus_tag="ACHJ69_000286"
     CDS             56286..57719
                     /gene="dacB"
                     /locus_tag="ACHJ69_000286"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO5013357.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLRRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(57760..58932)
                     /gene="cgtA"
                     /locus_tag="ACHJ69_000287"
     CDS             complement(57760..58932)
                     /gene="cgtA"
                     /locus_tag="ACHJ69_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO5013358.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(58948..59913)
                     /gene="yhbE"
                     /locus_tag="ACHJ69_000288"
     CDS             complement(58948..59913)
                     /gene="yhbE"
                     /locus_tag="ACHJ69_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO5013359.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLANPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(60040..60297)
                     /gene="rpmA"
                     /locus_tag="ACHJ69_000289"
     CDS             complement(60040..60297)
                     /gene="rpmA"
                     /locus_tag="ACHJ69_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO5013360.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(60318..60629)
                     /gene="rplU"
                     /locus_tag="ACHJ69_000290"
     CDS             complement(60318..60629)
                     /gene="rplU"
                     /locus_tag="ACHJ69_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO5013361.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            60888..61859
                     /gene="ispB"
                     /locus_tag="ACHJ69_000291"
     CDS             60888..61859
                     /gene="ispB"
                     /locus_tag="ACHJ69_000291"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO5013362.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            62087..62365
                     /gene="sfsB"
                     /locus_tag="ACHJ69_000292"
     CDS             62087..62365
                     /gene="sfsB"
                     /locus_tag="ACHJ69_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO5013363.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(62413..63672)
                     /gene="murA"
                     /locus_tag="ACHJ69_000293"
     CDS             complement(62413..63672)
                     /gene="murA"
                     /locus_tag="ACHJ69_000293"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO5013364.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(63727..63981)
                     /gene="ibaG"
                     /locus_tag="ACHJ69_000294"
     CDS             complement(63727..63981)
                     /gene="ibaG"
                     /locus_tag="ACHJ69_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO5013365.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(64141..64434)
                     /gene="mlaB"
                     /locus_tag="ACHJ69_000295"
     CDS             complement(64141..64434)
                     /gene="mlaB"
                     /locus_tag="ACHJ69_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO5013366.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEIREEAVKGITSIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(64434..65069)
                     /gene="mlaC"
                     /locus_tag="ACHJ69_000296"
     CDS             complement(64434..65069)
                     /gene="mlaC"
                     /locus_tag="ACHJ69_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO5013367.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(65088..65639)
                     /gene="mlaD"
                     /locus_tag="ACHJ69_000297"
     CDS             complement(65088..65639)
                     /gene="mlaD"
                     /locus_tag="ACHJ69_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO5013368.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(65644..66426)
                     /gene="mlaE"
                     /locus_tag="ACHJ69_000298"
     CDS             complement(65644..66426)
                     /gene="mlaE"
                     /locus_tag="ACHJ69_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO5013369.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(66434..67243)
                     /gene="mlaF"
                     /locus_tag="ACHJ69_000299"
     CDS             complement(66434..67243)
                     /gene="mlaF"
                     /locus_tag="ACHJ69_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO5013370.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            67453..68430
                     /gene="yrbG"
                     /locus_tag="ACHJ69_000300"
     CDS             67453..68430
                     /gene="yrbG"
                     /locus_tag="ACHJ69_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO5013371.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPREGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFIIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            68444..69430
                     /gene="kdsD"
                     /locus_tag="ACHJ69_000301"
     CDS             68444..69430
                     /gene="kdsD"
                     /locus_tag="ACHJ69_000301"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO5013372.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            69451..70017
                     /gene="kdsC"
                     /locus_tag="ACHJ69_000302"
     CDS             69451..70017
                     /gene="kdsC"
                     /locus_tag="ACHJ69_000302"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO5013373.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTHIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            70014..70589
                     /gene="lptC"
                     /locus_tag="ACHJ69_000303"
     CDS             70014..70589
                     /gene="lptC"
                     /locus_tag="ACHJ69_000303"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO5013374.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            70558..71115
                     /gene="lptA"
                     /locus_tag="ACHJ69_000304"
     CDS             70558..71115
                     /gene="lptA"
                     /locus_tag="ACHJ69_000304"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO5013375.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            71122..71847
                     /gene="lptB"
                     /locus_tag="ACHJ69_000305"
     CDS             71122..71847
                     /gene="lptB"
                     /locus_tag="ACHJ69_000305"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO5013376.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEYVKRVYLGEDFRL"
     gene            71895..73328
                     /gene="rpoN"
                     /locus_tag="ACHJ69_000306"
     CDS             71895..73328
                     /gene="rpoN"
                     /locus_tag="ACHJ69_000306"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO5013377.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            73351..73638
                     /gene="hpf"
                     /locus_tag="ACHJ69_000307"
     CDS             73351..73638
                     /gene="hpf"
                     /locus_tag="ACHJ69_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO5013378.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            73756..74247
                     /gene="ptsN"
                     /locus_tag="ACHJ69_000308"
     CDS             73756..74247
                     /gene="ptsN"
                     /locus_tag="ACHJ69_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO5013379.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LAPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            74293..75147
                     /gene="rapZ"
                     /locus_tag="ACHJ69_000309"
     CDS             74293..75147
                     /gene="rapZ"
                     /locus_tag="ACHJ69_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO5013380.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            75144..75416
                     /gene="npr"
                     /locus_tag="ACHJ69_000310"
     CDS             75144..75416
                     /gene="npr"
                     /locus_tag="ACHJ69_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO5013381.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            75630..76262
                     /gene="yrbL"
                     /locus_tag="ACHJ69_000311"
     CDS             75630..76262
                     /gene="yrbL"
                     /locus_tag="ACHJ69_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO5013382.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVTPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(76259..76987)
                     /gene="mtgA"
                     /locus_tag="ACHJ69_000312"
     CDS             complement(76259..76987)
                     /gene="mtgA"
                     /locus_tag="ACHJ69_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO5013383.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAIFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEQA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(76984..77637)
                     /gene="elbB"
                     /locus_tag="ACHJ69_000313"
     CDS             complement(76984..77637)
                     /gene="elbB"
                     /locus_tag="ACHJ69_000313"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO5013384.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAILPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(77867..80203)
                     /gene="arcB"
                     /locus_tag="ACHJ69_000314"
     CDS             complement(77867..80203)
                     /gene="arcB"
                     /locus_tag="ACHJ69_000314"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO5013385.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRALTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFERMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(80299..81228)
                     /gene="yhcC"
                     /locus_tag="ACHJ69_000315"
     CDS             complement(80299..81228)
                     /gene="yhcC"
                     /locus_tag="ACHJ69_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562163.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO5013386.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARERGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVGLDRYLNEHGVQGSALGRPWLPPTA"
     gene            81903..86363
                     /gene="gltB"
                     /locus_tag="ACHJ69_000316"
     CDS             81903..86363
                     /gene="gltB"
                     /locus_tag="ACHJ69_000316"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO5013387.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEDHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLLKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGADIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRGLMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESSDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            86376..87794
                     /gene="gltD"
                     /locus_tag="ACHJ69_000317"
     CDS             86376..87794
                     /gene="gltD"
                     /locus_tag="ACHJ69_000317"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO5013388.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAIVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGK
                     THDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEDNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(87862..88326)
                     /gene="nanQ"
                     /locus_tag="ACHJ69_000318"
     CDS             complement(87862..88326)
                     /gene="nanQ"
                     /locus_tag="ACHJ69_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO5013389.1"
                     /translation="MMMGEVQSLPSAGLHPALQGALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(88323..89198)
                     /gene="nanK"
                     /locus_tag="ACHJ69_000319"
     CDS             complement(88323..89198)
                     /gene="nanK"
                     /locus_tag="ACHJ69_000319"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO5013390.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALDGDITDMVFITVSTGVGGGVVSGGKLRTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGANAKTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     TGLLGAALLAQGEIL"
     gene            complement(89195..89884)
                     /gene="nanE"
                     /locus_tag="ACHJ69_000320"
     CDS             complement(89195..89884)
                     /gene="nanE"
                     /locus_tag="ACHJ69_000320"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO5013391.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(89932..91422)
                     /gene="nanT"
                     /locus_tag="ACHJ69_000321"
     CDS             complement(89932..91422)
                     /gene="nanT"
                     /locus_tag="ACHJ69_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO5013392.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGISGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPILGALIAQRLDLGTALGSLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(91531..92424)
                     /gene="nanA"
                     /locus_tag="ACHJ69_000322"
     CDS             complement(91531..92424)
                     /gene="nanA"
                     /locus_tag="ACHJ69_000322"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO5013393.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(92546..93337)
                     /gene="nanR"
                     /locus_tag="ACHJ69_000323"
     CDS             complement(92546..93337)
                     /gene="nanR"
                     /locus_tag="ACHJ69_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO5013394.1"
                     /translation="MSPMNAFDPQAEDSTTTIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            complement(93445..93942)
                     /gene="sspB"
                     /locus_tag="ACHJ69_000324"
     CDS             complement(93445..93942)
                     /gene="sspB"
                     /locus_tag="ACHJ69_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO5013395.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDETHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(93948..94586)
                     /gene="sspA"
                     /locus_tag="ACHJ69_000325"
     CDS             complement(93948..94586)
                     /gene="sspA"
                     /locus_tag="ACHJ69_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO5013396.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(94981..95373)
                     /gene="rpsI"
                     /locus_tag="ACHJ69_000326"
     CDS             complement(94981..95373)
                     /gene="rpsI"
                     /locus_tag="ACHJ69_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO5013397.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(95389..95817)
                     /gene="rplM"
                     /locus_tag="ACHJ69_000327"
     CDS             complement(95389..95817)
                     /gene="rplM"
                     /locus_tag="ACHJ69_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO5013398.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(96037..97164)
                     /gene="zapE"
                     /locus_tag="ACHJ69_000328"
     CDS             complement(96037..97164)
                     /gene="zapE"
                     /locus_tag="ACHJ69_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO5013399.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHMPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHDELTELQGQSDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDEIRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            97358..97756
                     /gene="zapG"
                     /locus_tag="ACHJ69_000329"
     CDS             97358..97756
                     /gene="zapG"
                     /locus_tag="ACHJ69_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO5013400.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            97910..99277
                     /gene="degQ"
                     /locus_tag="ACHJ69_000330"
     CDS             97910..99277
                     /gene="degQ"
                     /locus_tag="ACHJ69_000330"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO5013401.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINANKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIISALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARILAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESL
                     YLLMR"
     gene            99367..100434
                     /gene="degS"
                     /locus_tag="ACHJ69_000331"
     CDS             99367..100434
                     /gene="degS"
                     /locus_tag="ACHJ69_000331"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO5013402.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINPRRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGM
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVNNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(100497..101435)
                     /gene="mdh"
                     /locus_tag="ACHJ69_000332"
     CDS             complement(100497..101435)
                     /gene="mdh"
                     /locus_tag="ACHJ69_000332"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO5013403.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            101870..102340
                     /gene="argR"
                     /locus_tag="ACHJ69_000333"
     CDS             101870..102340
                     /gene="argR"
                     /locus_tag="ACHJ69_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO5013404.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKELYEAILELFDQEL"
BASE COUNT        25075 a        27577 c        25601 g        24250 t
ORIGIN      
        1 ttgattaaca tcaacctttt ctcttctggc aggacgtcat tttgtgaatg caatcgtttt
       61 ccataaattc ttctccccct cataggcaac gaatagcatt ttgtgttgag gatcacaaaa
      121 cgaataattg ctggtcgccg cgataaggtc agacgaagtc aacaaggagg attttcgctg
      181 tgctttacat cagcgtaggc catagaatgt tgcttcgttg actgtaatgc cgggtaaatg
      241 agcatttttt gatagtgcga aagaaccctg cgaggaaaat aagcatctat tattgtttgt
      301 catgatgaca aggctggcat caatgaaagg cttctcaatt gcacatattt ttcacccttc
      361 aatgcacgca gtggttaaca atcacaatag aaatattgat tattggacgg taaaaattta
      421 ggttataacg cccaaatgat tcaacgatcg gacaatacct gcgaacttac aattaatgag
      481 agaaatatat gtataattac gaccttttaa atgcaatata gacttaataa cttcttacaa
      541 agtctgttat taataccgta gatattcatc ttaccctggg caccaaaagc aaaaacccgc
      601 cgaagcgggt ttttacgtaa atcaggtgaa actgaccgat aagccgggtt ctgtcgtgga
      661 cagtcattca tctaggccag caatcgctca ctggctcaag cagcctaccc gggttcagta
      721 cgggccgtac cttatgaacc cctatttggc cttgctccgg gtggagttta ccgtgccacg
      781 gactgttacc agccgcgcgg tgcgctctta ccgcaccctt tcacccttac ctgatcccgc
      841 ttacgcgggc catcggcggt ttgctctctg ttgcactggt cgtgggtttc ccccccaggc
      901 gttacctggc accctgccct atggagcccg gactttcctc ccctccgccc gtctcccccg
      961 aagaggacga cgacgaagcg gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt
     1021 tgcgcgccct tgtcaccccg cgttgcgcat tccaatcgcc agtgtcgcgg cgatattacg
     1081 tgaagcacgg cagatattgt cataagctcc gcggagtgct tcgtctaacg taccaatgct
     1141 ggtcagtacg ctgaagaccg catcaatgcc atgctgatgt acaacgccaa catcattggt
     1201 caggctaccc gcaatgccaa tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc
     1261 aatcggaacc ttgccgtgta tactctggct gtcaatacgc ccttcaccgg tgatcaccag
     1321 cgtacaatcg tgaatatgtt cctccagatt cagcgccgtc gtgacgattt caataccact
     1381 tttcagttct gcaccaagaa acgccatcag cgccgcgccc atacctcccg cagctcctgc
     1441 gccggggaca tctttcacat caacatgcag cgctttttta ataacatcgg catagtgaga
     1501 gaggttattg tccagctcga caatcatcgc ctcactggct cccttttgtg ggccaaagat
     1561 gcgcgaagca ccactgtcgc cgactaacgg gttggtgaca tcacaagcga cgcggatgac
     1621 acaatctttt aagcgcggat cgagaccgga aatatcaata tcattcagag tattaagact
     1681 accgccgcca aaaccaattt cattgccgtt ggcatcgcat aatttcgccc ccagcgcctg
     1741 taccatgcct gcgccgccgt cgttggtggc gctgccgcca atgccgataa taatgtttgt
     1801 cgcaccgctc tccagcgcct gcaggattaa ctcaccagta ccgcgtgaag tggtcacgag
     1861 tggatcgcgt ttttccgcag gtaccagttc cagcccactg gccgccgcca tttcgataaa
     1921 cgcggttttg ccatcgccgg agatccccca actggcattc actttctcgc ccagcggccc
     1981 tgtaacccag gcgtgacgtt cagccccctg ggtggctgca atcatcgctt ccaccgttcc
     2041 ttcaccaccg tcggcaaccg gaacagaaac atactgtgca tcaggaaaaa tttcccgaaa
     2101 tcctttttct atcgcctgcg caacctcgct ggcagataaa ctttctttat aagagtctgg
     2161 ggcgattacg attttcatac ctatgcctgt taccacatga cgccggaggg cgtttctctt
     2221 attcggcctg gattccaggc ccggattgca atacgccatc cgggcacgac gtcattagcg
     2281 agtaacttcg actttcgcca gtttttcgta gtagcacgcc agggcgctat gatccgccgt
     2341 tcctaaacca tctgcacgca gtgcctgcat catctccata accgcagctg tgagcggcag
     2401 ttgcgcgccg acgccgtgag aagtatccag cgcattcgcc agatccttaa tatgcagatc
     2461 aatacggaag cccggtttga agttgcggtc catcaccatc ggcgctttgg catccagcac
     2521 ggtactgccc gccagcccac cgcgaattgc ctgatacacc agatccgggt taacgcccgc
     2581 tttggttgcc agcgttaacg cttctgacat cgcagcaata ttcagcgcca caatgacctg
     2641 atttgccagt ttggtgacgt tacctgcacc gatttccccg gtatgcacca cggaacccgc
     2701 catcgctttc atcaaatcat agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac
     2761 tgacagcgta ccgtcgatgg ctttcggttc accgccgctc accggagcat ccagcatatc
     2821 aatgcctttc gctttcagcg cttcgctgat ttcacggctt gccagcggtg cgatagaact
     2881 catatcgatc aataccgtac ctggcttcgc gccttcaata atgccattct cacccagcgc
     2941 cacctctttc acatgagggg agtttggcag catggttatg attacgtcgc actgttcagc
     3001 gatcgcttta gccgtagagg ctgtttctgc acctgcagca atcacgtcag cgatagcttc
     3061 tgggttacgg tcagcaacca ccagcgagta acctgctttc aaaaggtttt tactcattgg
     3121 tttacccata atcccaaggc caataaaacc aactttcata gtcatatcaa tcatctctct
     3181 tattgcggtg gtggttattt tttaaaggta tcagccagtt tctgagtggc agagcggaag
     3241 acgccgagat cgctgccgac ggccacaaac gtcgcgcccc attccagata acgacgcgca
     3301 tcagcctcga ctggtgcgag gatgccgctg ggtttgccgt gcgcgctggc acggttaaaa
     3361 atgtgctgaa ttgctttttg tacatccggg tgcgaggcat tgccgagatg gcctaatgcc
     3421 gcagccagat cgctggggcc gacgaagatg ccgtctacac cttcggtagc ggcaatagca
     3481 tcgacgttat ctacgccctg ctgactttct atctggacca gaatagtgat gttcttgttc
     3541 gactgagcga aataatccgc cacggtgcca aacatattgg cgcggtgaga aacggagacg
     3601 ccgcgaatgc cttccggtgg gtaacgagtt gatgccaccg cctgctctgc ttcctctttt
     3661 gtttctacaa aaggaatcag gaagttatag aaaccgatat ccagaagacg cttaataatt
     3721 accggctcgt tggtcggcac tcgcactact ggcgcgctgg cgctgccttt cagggccatt
     3781 aactgcggaa taaacgtgga gatatcgttt ggcgcatgtt caccatccag caccagccag
     3841 tcaaacccag ccaaaccaag aacttcagtg ctaatcgggt tagagagagc tgaccagcaa
     3901 ccaatttgta cctgtttcgc agccagtgcg gctttgaatt tattcgggaa aacatcgtta
     3961 ttcatcgctt atacctttgc ttatttctgc aattccatac gtttaatgtc gccgactacg
     4021 aagaggtagc agaccatcgc catcagcgct gaacatccca caaaaaccag tgctgcattg
     4081 aaggagtgca gttcacttac caggtagcca atcaccagtg gagtaacaat ggaggcaaca
     4141 ttgccaaaga cgttaaagac gccgccgcag aggccaacaa tctctttcgg cgcggtgtca
     4201 gaaatcactg gccagcccag cgcaccaaat cctttaccaa agaaagccag cgccatcagc
     4261 atgaccacca gcgtggtgtt gttggtgtag ttacataaga tgatggtgga agccagcaac
     4321 attcccagca caattggtag cttacgagcc agggtcaggg acaaaccgcg tttgatcaga
     4381 taatccgaga agacacctcc cagcacgccg cccgcaaagc cacacagtgc cggaatcgag
     4441 gcgaccagac ccactttcag aatcgacatg cctttttcct gcaccagata aattgggaac
     4501 caggtgagga agaaccaggt gatggtgttg ataaaatatt gtccgaaaaa tacgcccagc
     4561 atcatgcggt tagagagcaa ttgcttgatg taatgcagtt tgggtccgct tgctgccgca
     4621 ctgcccggct ttttgtggtc catatcgacc accgcgccat tttccgagat aaacttcagc
     4681 tcttccgcag acatacgtgg gtgatctgtc ggattatgaa tcaacttgat ccacagcgcc
     4741 gtcagcacaa aaccaatcac ccccataacg gtaaagacgt gctcccagcc ccaggcgaaa
     4801 gtcagccagc caagcagcgg cgaaaagagc gccaacgaga aatattgcgc cgagttaaag
     4861 atagcggagg cagtaccacg ttctttcgtc gggaaccagg cggcgacaat acgggcgttc
     4921 gccgggaatg atggcgcttc cgagaacccc agcataaatc gcataaagaa catggagatc
     4981 ccagcccagg ccagcggaaa catatcgaca aagccttgca ggaaggtgaa cagcgaccag
     5041 aaaaagaggc tgtaagtgta aactttcttc gagccaaact tatcaagcag ccagccgccg
     5101 ggaatttgca tcagcaagta ggcccagcca aaagcagaga agatgtaacc catcgaaacc
     5161 gcacttaact gcaactcttt tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg
     5221 gcgtagttaa cggcggtaac aataaaaata atcagtaata ttaaatagcg ggtatgcacg
     5281 cctttctttt tttcgtcaac ggtgtccagc atcattttat ttacctcggg tacttatgct
     5341 gatttttatt attatgggga aggtgttatt tatgagtttc atttatgccg ttacgacaat
     5401 gaactcggga attagtataa gcagcgcgag aataataatc attgtgcaaa tgctaattta
     5461 attaatacta tttaaatatt attttgagca tatgcacata aggttgcgcg ctaaagcaca
     5521 gatttgcgct ttaccttgcc gggaggtact gcaatccctg aaatgattga cattgatcac
     5581 atttctgcat ttaaactcct gacattctta tttcacccaa tgaagttatt tatttttaaa
     5641 tgagaccagg tcctcatttt aatagtccct ggctggagaa tattgcacaa tggccaacat
     5701 caaaatcaga caagaaacgc caactgcgtt ttatataaaa gttcacgaca cagataatgt
     5761 ggcaattatt gttaatgata atggcctgaa agcaggaacg cgttttccgg atgggctgga
     5821 attaattgaa catattcccc aagggcataa agtcgcattg ctggacattc cggctaatgg
     5881 tgaaattatt cgttatggcg aggtgattgg ttacgccgtg cgcgcaatcc cacgcggaag
     5941 ctggatcgac gaatcaatgg ttgtactacc ggaagcgccg ccgttacata cgctgccact
     6001 ggcaaccaaa gtcccggaac ccttaccgcc gctggaagga tacacctttg agggctatcg
     6061 caatgccgat ggcagcgtgg gcaccaaaaa cctgctcggc atcaccacca gcgtccactg
     6121 tgtggcaggc gtggtggact acgtagtaaa aatcattgaa cgcgatctgc taccgaaata
     6181 cccgaacgtc gatggcgtgg tggggctgaa tcatttgtac ggttgtggcg tggcgattaa
     6241 cgcacccgcg gcagttgtac ctattcgtac cattcacaat atttcgctga accctaactt
     6301 tggcggcgaa gtaatggtga ttggtctggg ttgtgaaaag ttgcagcctg aacgcctact
     6361 gactggaacg gatgatgtgc aagctattcc agtagaaagc gccagcattg tcagtttgca
     6421 ggatgaaaag catgtcggtt ttcagtccat ggtcgaggat attttgcagg tcgccgaatg
     6481 ccatctacaa aaactgaatc aacgtcagcg agaaacctgt ccggcttcag aactggttgt
     6541 cggcatgcag tgcggtggca gcgatgcgtt ttctggcgta acggcaaacc cggcggttgg
     6601 gtatgcgtct gatctattgg tgcgctgcgg cgcaacggtg atgttttcag aagtcacaga
     6661 agtgcgtgac gcgatacatc tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg
     6721 gctgctggag gagatggagt ggtacgataa ctatctcaat atgggaaaaa ccgatcgcag
     6781 cgccaaccct tcgccgggga acaagaaagg cggtctggca aatgtggtgg agaaggcact
     6841 cggctccatt gctaaatcgg gtaaaagtgc aattgttgaa gtgctgtcgc ccggccaacg
     6901 cccgactaaa cgcggattaa tttacgccgc gacgccagcc agcgattttg tctgtggcac
     6961 gcaacaggtg gcttcgggta tcaccgtgca agtgtttacg accggtcgtg gtacgccgta
     7021 cggcctgatg gcggtacccg tcattaaaat ggcgacccgc accgagctgg cgaaccgctg
     7081 gtttgattta atggatatta acgcgggcac tatcgctacc ggcgaagaaa ccattgaaga
     7141 agtgggctgg aagttgtttc actttattct cgacgtcgcc agcgggaaga agaaaacttt
     7201 ctcggatcaa tggggactgc ataaccagct ggcggtgttt aacccggcac cggtgacctg
     7261 atttcttttg gaacgtccct cgcaaaacat ggccttagtg ccatgttttt attgtttaaa
     7321 gtccacacat ccattaataa tgcatttgca ttaccttagt tcaagcttat aatttgagca
     7381 gaaaacaggc tgtaaaagga cagtaaataa tgcccgctaa tgctcgctct aacgctgtac
     7441 tgaccactga atcaaaggtc actatacgcg gacaaacaac tatccccgcg ccagtgcgtg
     7501 aggccttaaa actgaagcct ggcctggaca gcattcatta cgaaattctg cctggtgggc
     7561 aagtatttat gtgccgactg ggagatgaac aggaggatca tactatgaat gcatttttgc
     7621 gttttctgga tgcagatatc cagaacaacc cgcaaaaaac tcgtccattc gacattcaac
     7681 aaggaaagaa acttgtcgct ggcatggacg tcaacattga cgatgagatt ggcgacgacg
     7741 aataatggat tttccacaaa gggttaatgg ttgggcgcta tatgctcatc cctgttttca
     7801 ggaaacctac gatgctttag ttgccgaagt cgaggcatta aagggaaaag atcctgaaaa
     7861 ttatcagaga aaagctgcca caaagttatt ggcggtggtc cataaagtga ttgaggagta
     7921 tatcacggtc aatccatcat caccggcatt ccgtcatggc aagtcgttag gctctgggaa
     7981 aaataaagac tggtcacggg taaaatttgg cgctggtcgc tatcgtctct tctttcgtta
     8041 cagtgaaaaa gagaaagtca tcattctggg atggatgaac gatgaaaaca cgctgcgcac
     8101 ctacggtaaa aaaacagatg cctataccgt attcagtaaa atgttaaaaa gaggacatcc
     8161 tcctgccgac tgggaaagcc tcacccaaga gacagaagaa aaccattgat ggtgttcaca
     8221 atgcccgccg aacgcattgg tgttgaccag cgggcatcct ttctcattcc ccgaccagaa
     8281 tcacttcaac cccagccttt ctcagtcctt ccagactatc cgcaggaatg ccttcatcaa
     8341 caatgatcat gtcgatacgt tgagtatcaa tgatcttatg taaactagaa cgattgaact
     8401 tactggaatc ggtgaccacg atgatccgtt ccacaacttc acacatccga cggtttaaac
     8461 gggcttcatc ttcattatgc gtgctgacgc cgcgctccag atcgatcgca tctacaccaa
     8521 gaaacagcat atcgaagtgg taattttgca gcgattgctc agcctgatca ccgtaaaaag
     8581 attgcgactg acggcgcaaa tgccccccgg tcatcagcag ctcaacgcct tccgcttcca
     8641 gcaacgcatt agccacgttc ataccgttgg tcatcgcaat tacgtcagtg tgcttgcgca
     8701 tcagacgagc aatctcaaaa gtggtggtcc cggagtcgag gatcacccga tgaccaggct
     8761 gaatcaactc aacggcagct ttcgcaacgc tgcgtttcat cgcggtgttc agcgcgcttt
     8821 tatcttccac tgatggctcg actgacggcg ttgtgctatc gcagatcaac gcgccaccat
     8881 aggcacgcac agcgatcccc tgcttttcca gaaacgccag atcgttgcgg atcgtcacag
     8941 tagatacacc atacaacgcc gatagatcat taacttgcac acttccttgc tggcgcaggc
     9001 gttgaatgat ctgttctcgt cgctcgctgg tgcctgtcac tcgcttctca cctgaagcgt
     9061 cggtattact catagtaagt cctttcgtaa aactttcgtt tcatttcgtt ttgcctatta
     9121 acgcctttct attaagcaaa tgcaagccca ccttacccat tggcgcaagc tactctcgtt
     9181 tcagtagctt tcattatgtt tcttttgtga atcagatcag aaaactatta tctttcgttt
     9241 tatttttatc tcaccatgac gcagtatcaa ctgaaacaaa acgaaagatt aatgtcgcag
     9301 taatctgaac tggagaggaa agtgaaacat ctgacagaaa tggtgagaca gcacaaagcg
     9361 ggcaaaacaa atgggattta tgccgtttgt tccgcacatc cgctggtgct ggaaactgca
     9421 atccgctacg ccagtgcaaa ccaaacgccg ttactgattg aagcaacctc caatcaggtg
     9481 gaccagttcg gcggttatac cggaatgacg cccgccgatt ttcgcggctt tgtttgtcag
     9541 ctcgccgact cgttgaattt cccgcaggat gcgctgattc tgggtggcga ccatctgggg
     9601 ccaaaccgct ggcaaaacct gcctgccgct caggcaatgg ccaatgccga tgatttgatt
     9661 aaaagctacg tggcggcagg attcaaaaaa attcaccttg attgcagcat gtcctgtcag
     9721 gacgatccga ttcccttaac tgatgacatc gtggctgaac gcgccgcccg tctggcgaaa
     9781 gtggcggaag aaacctgtct tgaacacttt ggcgaagccg atctggagta tgtcattggt
     9841 accgaagtcc cggtgcctgg cggcgcgcat gaaaccttaa gcgagctggc ggtcaccacg
     9901 ccggatgccg cccgcgccac gctggaagcc catcgtcacg cttttgaaaa gcagggtctg
     9961 aatgccatct ggccacgcat cattgccctg gtggttcaac ccggcgtcga attcgatcac
    10021 accaacgtta ttgattatca gcccgccaaa gcgaccgcct taagccagat ggtcgaaaac
    10081 tacgaaacgc tgattttcga agcgcactct accgattatc aaacaccgca atcgctgcgc
    10141 cagctggtga ttgaccactt tgccattctg aaagttggcc cggcgctgac ctttgctctg
    10201 cgtgaagctc tgttctctct ggcggcgatt gaagaagaac tggtgccagc aaaagcctgt
    10261 tctggtctgc gtcaggtgct ggaaaacgtg atgctcgacc gcccggaata ctggcaaagc
    10321 cactaccacg gtgacggcaa cgcgcgtcgt ctggcgcgtg gttatagcta ctcggatcgc
    10381 gtgcgctatt actggccgga cagccagatt gatgacgctt tcgctcatct ggttcgtaat
    10441 ctggcggatt caccaattcc gctgccgctg atcagtcagt atctgccgct gcagtacgtg
    10501 aaagttcgct ccggcgagct gcagccaacg ccacgggaac tcattatcaa ccatattcag
    10561 gacatcctgg cgcagtacca cacagcctgt gaaggccaat aagcaaaaca aagaggaaca
    10621 cgctatgcca aatattgttt taagtcggat tgatgaacgc ttgattcacg gtcaggtcgg
    10681 cgttcagtgg gtcggatttg cgggggcaaa tctggtgctg gtagccaacg atgaggttgc
    10741 cgaagatctg gtacaacaaa acctgatgga aatggtactg gcagaaggga tcgccgtacg
    10801 tttctggacg ctgcaaaaag tcatcgacaa cattcatcgc gccgccgatc gacagaaaat
    10861 cctgctggtt tgtaaaacac cggccgattt cctgacgctg gtgaaaggtg gcgttccggt
    10921 gaatcgcatt aacgttggca atatgcacta cgccaatggc aagcaacaaa tcgccaaaac
    10981 ggtttctgtg gatgcgggtg atatcgcagc atttaacgac ctgaaagccg ctggggtgga
    11041 atgcttcgtt cagggcgtcc cgacagagcc tgcagtggac ctctttaaat tactttgagg
    11101 gattcatcat ggaaatcagt ctgttgcagg catttgcgtt gggaattatc gcctttatcg
    11161 ctggcctgga tatgtttaac ggcctaaccc atatgcaccg cccggtggtc ctcggcccgt
    11221 tggtcggact ggtacttggc gatctgcata ccggtatttt aaccggcggt acgctggaac
    11281 tggtgtggat ggggctggcc ccgctggcag gcgcacaacc gcctaacgtg attatcggta
    11341 ctatcgtcgg cacggcgttt gccattacta ctggcgtgaa acccgatgtc gcggtcggtg
    11401 tcgccgtacc tttcgctgtc gcagtacaga tggggattac cttcctgttc tcggtgatgt
    11461 ctggcgtgat gtcccgctgc gaccggatgg cggagaacgc cgatacacgc ggcattgaac
    11521 gcgtgaacta tctggcattg ctggcactcg gcactttcta ttttctctgc gctttcctgc
    11581 ctatctactt cggcgcagaa catgccaaaa ccatcattga tgtcctgccg caacgattaa
    11641 tcgacggcct cggcgtcgcg ggcggcatca tgccagcaat cggttttgcc gtgctgctga
    11701 aaatcatgat gaaaaacgtc tacatcccct acttcatcct gggctttgtt gccgcagcct
    11761 ggctcaagtt accggtgctg gctattgctg ccgccgcgct ggcgatggcg ctgatcgacc
    11821 tgctgcgtaa atctcctgaa ccgactcaac ctgcggcaca gaaagaggaa tttgaagatg
    11881 gcatctaatc aaaccaccct gccgaacgtc agcgaaaatg aagaaacact gctgactggc
    11941 gtcaatgaaa acgtgtatga agatcagagc attggcgcgg agctgacgaa aaaagatatc
    12001 aaccgcgtcg cctggcgttc catgctgtta caggcttctt tcaactacga acgtatgcag
    12061 gcttccggct ggctgtacgg cctgctgcca gcactgaaaa agatccacac taataaacgc
    12121 gacctggcgc gcgccatgaa ggggcatatg ggtttcttca atacccatcc gtttctggtg
    12181 acgtttgtta tcggcattat ccttgcgatg gagcgttcta agcaggacgt taacagtatt
    12241 cagagcacca aaattgccgt cggtgcgccg ctcggcggga ttggcgatgc gatgttctgg
    12301 ctaacgctac tgccgatttg tggcgggata ggtgcaagcc tcgcgctaca aggctctatt
    12361 cttggcgctg tcgtctttat tgtgctgttc aacgtggtac acctggggct gcgttttggt
    12421 ctggcgcatt atgcttaccg catgggcgtg gcggctattc cactcattaa agcaaatacc
    12481 aaaaaagtcg gccatgcggc atctatcgtt gggatgacgg taatcggcgc gctggtggca
    12541 acctatgttc gtttaagcac cacgctggaa atcacagcgg gcgacgcagt ggttaagtta
    12601 caggctgatg ttatcgacaa actgatgcct gccttcttac cgctggtcta caccctgacc
    12661 atgttctggc tggtacgccg cggctggagt ccgctgcgcc tgattgcagt gaccgtggtt
    12721 ctcggcatcg tcggtaaatt ctgccatttc ctttaaatac aatgaggtat tagatgttaa
    12781 gtattatttt gacagggcat ggcgggtttg ccagcggtat ggaaaaagca atgaagcaaa
    12841 ttctcggtga gcaatcgcag tttatcgcca tcgattttcc ggaaacctca agcaccgcac
    12901 agcttacatc gcagcttgaa gaggccatcg cccaactgga ttgtgaagac ggcattgttt
    12961 ttctgacgga tttgctgggc ggcacgccgt ttcgcgtagc ttcaacgctg gcgatgcaaa
    13021 aaccgggctg tgaagtgatc acagggacca atttacaact gctactagag atggtgctgg
    13081 aacgcgaagg gttaagcggt gaagagtttc gtgtgcaggc gctggagtgt ggacaccgtg
    13141 ggctaaccag tctggtggac gaacttggtc gctgccatga ggaatgtccg gtcgaggaag
    13201 gaatatgaca cacgttctgc gcgccagaag gctgctgact gaagagggat ggttcgatga
    13261 ccatcagttg cgtattgctg acggtgtaat cgcagcaatc gaaccgattc cagtgggcgt
    13321 tactgaacgc gatgcggaac tgctctgccc cgcttacatc gacacccatg tacacggtgg
    13381 tgcgggcgtt gatgtaatgg atgacgcgcc ggatgcgctc gacaagctgg caatgcacaa
    13441 ggcacgcgaa ggtgtcggca gttggttacc gaccaccgta accgcgccgc ttaataccat
    13501 tcatgcggcg ctgaaacgta ttgctctacg ctgccagcgt ggcggacctg gtgcgcaagt
    13561 gctggggagt tatctcgaag gaccgtactt cacgccgcag aataaaggcg cgcatccgcc
    13621 ggagctgttt cgcgagcttg aaattgccga gctggatcaa ttgattgccg tttctcagca
    13681 taccttgcgc gtggtagcgc tggcaccaga aaaagaaggg gcattgcagg ccatccgcca
    13741 tcttaaacag caaaacgtac gggtgatgct ggggcatagc gcggcgacct ggcaacaaac
    13801 ccgtgccgcg tttgatgctg gtgccgacgg cctggtgcat tgctataacg ggatgacagg
    13861 tttacatcac cgcgaaccgg gaatggttgg cgcgggatta acagacaagc gcgcctggct
    13921 ggaactgata gccgatggtc atcatgtaca tccggcggca atgtcgctct gttgttgctg
    13981 tgcgaaagag aggatcgtac tgatcaccga cgcgatgcag gcagccggga tgccggatgg
    14041 tcactatacg ttatgtggtg aagaagtgca gatgcgcggt ggcgttgtcc gtaccgcgtc
    14101 cggtgggctg gcgggcagta cgctgtctgt cgatgcggca gtgcgcaaca tggtcgagtt
    14161 gacgggcgta acgcctgcgg aagccatcca tatggcgtcg ctgcatccgg cgcgaatgct
    14221 gggtgttgat ggtgttctgg gatcgcttaa accgggcaaa cgcgccagcg tcgttgcgct
    14281 ggatagcggg ctgcatgtgc aacaaatctg gattcagggt caattagctt cgttttgatg
    14341 gtttgctcct ttattaggcc ttcacttccc ccgtaaggcc tttctttttc tttcgttttg
    14401 atctgtgcag cggtgtcgga tgcgacgcta atgcgtctta tccgacctac ggttggtgag
    14461 cgtaaggccg gataaagtat ttacgccgca tccagcaatc ccttttactt cccttacctt
    14521 ttctttcaac gatcacagat ttcgttttat ttcctttttc tccattgaac tttcagtttc
    14581 ttttctatag attttaacca acgaaagaaa tcaccaagtg aaatgaaacg aaaggcaagc
    14641 gaaagcgaca acgccgacgt caagttcatc agactaagga ttgagttatg ccagaaaatt
    14701 acacccctgc tgctgccgca accggtacat ggactgaaga agagatccgc catcagcctc
    14761 gcgcatggat ccgttcactc accaacattg acgcgctacg ttccgcgctc aataacttcc
    14821 ttgaaccgtt actgcgcaaa gagaatctgc aaatcatcct gaccggagcc ggaacatcgg
    14881 catttatcgg tgacatcatc gcgccgtggc tcgccagcca taccggtaaa aacttcagcg
    14941 ccgtaccgac caccgatctg gtcaccaatc caatggacta cctgaaccca gctcatccgc
    15001 tactattgat ctccttcggt cgatccggca acagcccgga aagcgtcgcc gccgtgaaac
    15061 tggcaaatca atttgtacca gaatgctatc acctgccgat cacctgcaac gaagcgggcg
    15121 ctctttacca aaacgcgatc aacagcgaca acgcgtttgc cctgctgatg cccgcagaaa
    15181 cgcacgatcg cggcttcgcg atgaccagca gcattaccac catgatggcc agctgcctcg
    15241 cggttttcgc acctgagatg atcaacagcc aaaccttccg cgacgtggcg gatcgttgcc
    15301 aggcgatcct gacctcactg ggcgatttca gcgaaggtgt gtttggttac gcaccgtgga
    15361 aacggatcgt ttatctcggc agcggtggct tacagggcgc agcacgcgag tcggcgctga
    15421 aagtgctgga actgacagcg ggtaaactgg cggccttcta tgattccccg accggattcc
    15481 gtcatggccc gaaatcgctg gtcgataacg aaacgctggt ggtggtgttt gtctccagcc
    15541 acccttacac ccgtcagtat gatcttgatc tgctggcaga actccgccgc gataaccagg
    15601 caatgcgcgt aatcgccatc gccgcggaaa gcaacgacgt tattgccgcc ggtccacata
    15661 tcatcctgcc gccgtcccgt cactttatcg acgttgagca ggcattttgc ttcctgatgt
    15721 acgcccagac atttgcactg atgcagtcgc tgcacatggg taatacgccg gataccccat
    15781 cagccagtgg caccgttaac cgcgtggtgc aaggcgtaat cattcatccg tggcaggcat
    15841 aagaggatcg cattatgagc attatctcca ccaaatatct gttacaggac gcccaggcca
    15901 atggctacgc ggtgcctgcc tttaacattc ataacgccga gacgatccag gcgatcctcg
    15961 aagtgtgcag tgaaatgcga tcgccggtga tcctcgccgg aacgccgggg acctttaaac
    16021 atatcgcgct ggaagagatc tacgccctgt gtagtgccta ttccacaacc tacaacatgc
    16081 cactggcgct gcatctcgac caccacgaat cgctggatga tattcgccgt aaagtccacg
    16141 caggcgtgcg cagcgcgatg atcgacggca gtcatttccc gtttgccgag aacgtgaagc
    16201 tggtgaaatc ggttgtcgac ttctgccact cccaggattg cagcgtggaa gcagaactgg
    16261 gccgcctcgg cggtgttgaa gatgacatga gcgttgacgc cgaaagtgca ttcctgactg
    16321 atccacaaga agctaaacgc tttgtcgaac tgactggcgt cgacagcctg gcggtagcga
    16381 ttggtacggc gcacggctta tacagcaaaa cgccgaagat tgatttccag cggctggcgg
    16441 aaattcgtga agtggtggat gttcctctgg tgctgcatgg tgccagcgat gtcccggatg
    16501 aatttgtccg tcgcactatt gaacttggcg taacaaaagt gaacgttgcc acagaattaa
    16561 aaatagcctt cgctggcgcg gttaaagcct ggtttgcgga aaatccgcag ggtaatgatc
    16621 ctcgttatta tatgcgcgtc ggaatggatg cgatgaaaga agttgtcaga aataaaatta
    16681 atgtctgtgg ttcagcgaat cgaatttcag cataatcatt agttttttat gatttatccc
    16741 aatgtacttc ccgattaata tcggggagtg ccttaatggt aaaggagata actaaacctt
    16801 aataaataca tcagtacaat atcgcaacaa taatatattt aaaaagatta tattattcaa
    16861 ctttatggtg aggattacac aatgagcagt ccaaatattc tcttaacccg tattgataac
    16921 cgtctggttc acggtcaggt tggcgtgacc tggacatcca ccatcggtgc gaatctgtta
    16981 gtagtcgtgg atgatgttgt cgctaacgat gctattcaac agaaattaat gggtattacc
    17041 gcagaaacct acggctttgg cattcgtttc tttactatcg aaaaaaccat taacgtcatc
    17101 ggcaaagctg caccacatca gaagatcttc ctgatttgcc gtacgccaca aacggtacgt
    17161 aaattggtag aaggtggtat tgacctgaaa gatgtcaacg tcggcaatat gcatttctcg
    17221 gaagggaaaa agcaaatcag cagtaaagtt tatgtcgatg accaggatct cacggattta
    17281 cgttttatta aacaacgtgg cgtaaatgtt ttcattcagg acgtccctgg cgatcaaaaa
    17341 gaacaaatcc ctgactaaat ctaaaatagc cttaatattg gtttgaggta ataaaaatgc
    17401 atgaaataac cctactccag ggattatccc tggcggcgtt agttttcttt ctggggattg
    17461 atttttggct ggaagcctta tttttattcc gcccgataat cgtttgtacc cttacaggtg
    17521 ctattctcgg tgatattcag actggcttaa ttaccggtgg cctgacagag ttggctttcg
    17581 ccggattaac ccctgcaggt ggtgttcagc cgcccaaccc tattatggcg ggtctgatga
    17641 ccactgtcat tgcatggtct acgggcgttg atgccaaaac ggcaattggt cttggcctgc
    17701 cgtttagttt gttaatgcag tacgtcattc tgttcttcta ttccgctttc tcattattta
    17761 tgaccaaagc cgataaatgc gcaaaagagg cggatacggc agcgttttcc cgacttaact
    17821 ggacaacgat gctcatcgtc gcttcagcgt atgcggtgat tgctttcctc tgtacttacc
    17881 tggcacaagg ggcgatgcag gcgctggtga aagcgatgcc cgcctggctg acccacggct
    17941 ttgaagtggc tggcggtatt ctgcctgctg ttggttttgg cttgctgctg cgcgtgatgt
    18001 tcaaagcaca atatatccct tacctgatcg ccggtttcct gtttgtttgc tacatccagg
    18061 tcagcaacct gttgccggtt gccgtactgg gcgcaggctt tgcggtgtat gagtttttca
    18121 atgcgaaatc ccggcagcaa gcgcaaccgc agcccgttgc cagtaaaaat gaagaagagg
    18181 actacagcaa tgggatctga aatcagcaaa aaagatatca cccgtctggg ctttcgttcg
    18241 tcactgctac aagcaagctt taactacgaa aggatgcagg cgggcggttt tacctgggcg
    18301 atgttgccga tcctgaaaaa gatttataag gacgacaaac cgggcctaag cgcggcgatg
    18361 aaagataacc tcgaatttat taatacccac cctaatctgg tcggattcct gatggggtta
    18421 ttaatttcga tggaagaaaa aggagaaaac cgcgacacca ttaaaggcct caaagtggcg
    18481 ctgtttggcc caatcgccgg gattggcgat gcgattttct ggtttacctt gttgccaatt
    18541 atggcgggga tttgctcatc gtttgccagt caggggaacc tgttgggacc gattttgttt
    18601 ttcgccgttt acctgcttat ctttttcctg cgcgtcggct ggacccacgt cggttattca
    18661 gtcggcgtga aggctatcga taaagtgcga gagaactcgc agatgattgc tcgttcggca
    18721 accatcctcg ggatcacagt aatcggcggg ctgatcgctt cgtatgtgca tattaacgtg
    18781 gtgacatcgt ttgccatcga cagtacccac agcgtcgcgc tgcagcagga tttcttcgat
    18841 aaagtcttcc cgaatatttt accgatggcc tacaccctgc tgatgtatta cttcctgcgg
    18901 gtgaaaaaag cgcatccggt gctgttaatc ggcgtgactt ttgtgctctc tattgtttgt
    18961 tctgcattcg gcattttgta aatggaacga ggcactgcgt ctggtggtgc ctctttacta
    19021 aaggaatttc atcctgtgca aacccttcaa caagttgaaa actatacggc gttaagtgaa
    19081 cgtgccagcg aatatttatt ggccgtgatc cgtcgcaaac cggatgccgt gatttgcctg
    19141 gcgaccggag ccacgccatt actgacgtat cattatctgg tagaacaaat ccaccaacag
    19201 caggttaatg tcagccagct caccttcgtg aagctcgacg aatgggtgga tctgccatta
    19261 acgatgcctg gcacctgcga aactttccta caacagcata tcgtgcagcc gctggggctg
    19321 cgtgaagacc agctcatcag ctttcgctct gaagagataa atgagacaga gtgcgaacgg
    19381 gtaacgaacc tgattgcgcg caaaggcggt ctggatttat gcgttctcgg attggggaaa
    19441 aacggtcatc ttgggctgaa cgaaccggga gaaagcctgc aaccggcctg ccatatcagt
    19501 caacttgatg ccggaacaca gcaacatgag atgttaaaaa ccgcgggccg tcccgtgact
    19561 cgtgggatca ccttaggcct gaaggatatt ctcaataccc gcgaagtttt gctactggtg
    19621 actggcgaag gaaagcagga tgcgacagaa cgttttctca cggctaaagt ctctaccgct
    19681 atcccggctt cgtttttatg gctgcataac catttcacat gcgtgattga tgaaatgtgt
    19741 cgtcgttaac tcgcctggta aaggcgttgt agcgctattc ctgctgcaac gcctgtaatc
    19801 actctccctg ctgctccagt gcatacttat acagcgcatt tttcttcacg ccgtgaattt
    19861 ctgctgccag cgccgccgct tttttcagtg gcagttctgc ctgcaacagc gccagcgtgc
    19921 gcagggcatc ggcgggtaag tcttcttcct gtgctttatg gccttcgaca atcagtacca
    19981 tttcgccttt gcgacggttt tcatcttctt tgacccacgc cagcagctcg ccaacgggcg
    20041 cgccgtgaat ggtttcccag gttttggtca gctcacgcgc cagaacgacg tagcgggatt
    20101 cgcctaatac cgcaacgata tcttccaggc tatctaacag acggtgggta gattcataaa
    20161 aaatcagcgt gcgcggctcc gcttcaatgg cttttagcgc atcacggcgg ccttttgatt
    20221 tggcaggtaa aaagccttca taacaaaaac gatcggatgg taaacccgcg gcgcttaacg
    20281 cagtgatagc agcacacggc ccgggtagcg gcaccacgcg aatccccgct tcacggcagg
    20341 tacgcaccag atggtagcca ggatcgttaa ttagcggcgt tccggcatcg gaaaccagcg
    20401 caatgttttg cccctcttgc agcttcgcca gcagcgtttc ggctttttgt tgttcgttat
    20461 ggtcgtgcag cgcaaacaac cgggcattaa tcccaaaatg ttgcagcaat aaaccggtgt
    20521 gacgcgtatc ctcggcggca atcagatcaa cggcctgtaa tacctccaac gcacgctggg
    20581 tgatatccgc cagattgccg attggcgtcg gtacaatgta aagctggccc tgagaattat
    20641 ccgccgattg gtgttgtttc attgtgtcgt ccgtattgcc gatttaatat tgagcattgc
    20701 gtaaaaaaaa tatcactgga tacattatgg taccctcaac attttctcgt ttgaaagccg
    20761 cgcgttgtct gcctgttgtt ctggcagccc tgattttcgc cggttgtggc acccatactc
    20821 ccgatcagtc cactgcttat atgcagggca cggcgcaggc tgattctgcc ttttacctgc
    20881 aacagatgca gcaaagttct gatgatacca ggatcaactg gcaattactc gccattcgtg
    20941 cactggtgaa agaaggtaaa accggacagg cggtggagtt gtttaaccaa ctaccgcaag
    21001 aactgaacga ttctcagcgt cgcgagaaaa cactgctggc ggcagagatt aaactagcgc
    21061 agaaagattt tgctggcgcg caaaacttgc tggcgaaaat cacacctgca gatttagaac
    21121 aaaaccagca agcgcgttac tggcaggcaa aaatcgatgc cagccagggg cgtccttcca
    21181 ttgatttact gcgcgcgtta attgctcagg aaccgctgct cggcgcgaaa gaaaagcagc
    21241 agaatattga cgccacctgg caggcgctct cctccatgac tcaggaacag gcgaatacgc
    21301 tggtgatcaa tgccgacgaa aatattctgc aaggctggct ggatctgcag cgcatctggt
    21361 ttgataaccg taacgatcct gacatgatga aagctgggat cgccgactgg cagaaacgtt
    21421 atccgaacaa cccgggcgcg aaaatgctgc caacgcagtt ggttaacgta aaagcgttta
    21481 aaccagcatc gaccaacaaa atcgccctgc tgttgccgct gaatggccag gcagcggtat
    21541 ttggtcgcac tattcagcaa ggctttgaag cggcgaaaaa tatcggcact cagccagtgg
    21601 tggcccaggt agcagccgca cctgccgcag acgtagcaga acaacctcag ccgcaaaccg
    21661 tggatggcgt tgccagcccg gcacaagctt cggttagcga tctgaccggt gaacagcctg
    21721 cggcccagtc ggtgcctgta agcgccccgg cgacaagcac cgcagcggta agcgcacccg
    21781 caaatccatc cgcagagctg aaaatctacg atacctcatc acaaccactt agccagatct
    21841 taagccaggt tcagcaggat ggtgcgagta ttgtggtcgg tccgttgctg aaaaataacg
    21901 ttgaagagtt gctgaagagc aacaccccgc tgaacgtgct ggcactgaac cagccggaga
    21961 acatcgaaaa ccgcgtcaat atttgttact tcgcgctttc accggaagac gaagcgcgcg
    22021 atgcagcgcg tcatattcgt gaccagggta aacaagcgcc gctggtgctg atcccacgca
    22081 gttcattggg cgatcgcgta gccaatgcgt ttgcgcaaga gtggcagaaa ctgggcggcg
    22141 gccccgttct gcaacaaaaa tttggttcca ccagcgaatt acgcgcgggt gttaacggcg
    22201 gttcgggtat cgctttaacg ggtagcccga ttactcccac agaaacaacc gactccggca
    22261 tgacgaccaa caatccaacg ctgcaaacca cgccaaccga tgatcagttc accaataatg
    22321 gcggtcgtgt cgatgcggtg tacattgtgg caacgccggg tgaaatcgct tttatcaaac
    22381 caatgatcgc catgcgtaac ggtagccaga gcggtgcaac gctgtacgcc agctcccgca
    22441 gtgcgcaagg gaccgctggc ccggatttcc gcctggagat ggaaggcttg cagtacagcg
    22501 aaatcccgat gctggcaggc ggtaatctgc cgttaatgca gcaggcgctc agcgcggtga
    22561 acaacgatta ttcgctggct cgcatgtatg cgatgggcgt cgatgcctgg tcgttggcaa
    22621 atcatttctc acaaatgcgc caggttcagg gttttgaaat caacggtaat accggaagcc
    22681 tgacggcaaa cccggattgc gtgattaaca ggaagttatc atggctacag taccaacaag
    22741 gtcaggtagt ccccgccagt taaccaccaa acagaccggc gatgcgtggg aagcacaagc
    22801 gcgtcgctgg ctggaaggca aaggactacg gtttatcgcc gctaacgtga acgagcgtgg
    22861 cggcgagatc gatctgataa tgcgtgaagg ccggaccacc atttttatcg aggtgcgtta
    22921 ccgccgctct gcgctttatg gcggctcggc agccagtgtg acccgcagca aacaacacaa
    22981 attattacag actgcccgct tgtggctcgc gcgtcataat gggagttttg atactgtgga
    23041 ttgccggttc gatgtggtag ccttcaccgg gaatgaggtt gagtggatta aggatgcctt
    23101 taatgaccac tcataattaa ggtttaagga ttagcgtgca agaaagaatt aaagcttgct
    23161 tcactgaaag cattcaaact caaattgcgg cggcagaggc gcttccggat gccatctccc
    23221 gtgcagccat gacgctggtt cagtctctgc tcaatggcaa caaaatcctc tgttgtggta
    23281 atggaacttc cgctgccaat gcacagcatt ttgctgccag catgatcaac cgtttcgaaa
    23341 cggagcggcc cagcttacct gccattgcac taaatactga taatgttgtc ttaacggcaa
    23401 ttgccaacga tcgcttacat gatgaagtgt atgcaaaaca ggtgcgggcg ctgggtcatg
    23461 cgggagatgt attgttagcc atttccaccc gtggcaacag ccgcgatatt gtaaaagcgg
    23521 tagaagctgc cgttacgcgt gatatgacca ttgtggcatt aaccggctat gacggcggtg
    23581 aacttgcagg tttgttaggg ccacaggatg tggagatccg cattccttcg catcgtagtg
    23641 ctcgcattca ggaaatgcat atgctgacgg taaattgcct gtgcgatctg atcgataaca
    23701 cgcttttccc tcaccaggat gtttaaggag aatacatgaa ggcattatcg ccaatcgcag
    23761 tccttatttc cgcgctgctg ttgcaaggtt gtgttgccgc tgccgtagtg ggtactgctg
    23821 ctgtgggtac caaagccgca actgacccac gcagtgtcgg cacccaggta gacgatggta
    23881 ccctggaagt gcgcgtgaac agcgcattgt cgaaagacga acaaattaag aaagaagcgc
    23941 gcattaatgt aacggcctat cagggcaaag tgctgctggt tgggcagtca ccaaacgctg
    24001 aactttcggc tcgcgccaaa cagattgcta tgggcgtaga cggtgccaac gaagtgtata
    24061 acgagattcg tcagggccag ccgattggtc tgggcgaagc gtctaacgat acgtggatca
    24121 ccaccaaagt acgttcgcag ctgttaacca gcgacctggt gaaatcgtcc aacgtgaaag
    24181 tgaccaccga aaacggtgaa gtgttcctga tggggctggt gactgaacgt gaagcgaaag
    24241 cggcggcaga tattgccagc cgggtaagcg gcgtgaagcg ggtaactacg gcgtttacgt
    24301 ttattaaata gcagcccttg taatgcctga tgcgacgctt acagcgtctt atcaggccta
    24361 caacgatgca aaccgtaggt cggataaggc gtttacgccg catccgacat gttttccctc
    24421 aaatcaaacc aacagcgcca gcccgccgac aatcacacca gacactgcca ccatcgcccc
    24481 cgtcagccat aaggctttcg ccggaaacgc tttacgcaac attatcagcg acggcaaact
    24541 caccgccggg agcgtcatca acaatgccag cgccggagcg gttcccatac ctgccagcat
    24601 catcgtttgt acaatcggaa tttctgctgc cgtgggaatg acaaacaagc atcccgctac
    24661 cgccatcgcc accacccaca tcaggctgtt atcgacagca ccatcggcat gggggaataa
    24721 ccagacgcga gcggcaccca acaccagtac tgcaaggatg taaaccggga tcgtgctcca
    24781 gaaaagcgtc catagcgccc tgccccagcg gctaaaaaaa ccgccctgtg attccggtat
    24841 gtcaatttcg accggtgcct gcgtttgcgg tgtttcacgc acccattttt gcaccagcgt
    24901 cgcaatcagc aacaccatca ccagccccgc caccagacga atcgccgcaa aaccccagcc
    24961 gaggacgaac cccataaaca ccagcgtcgc cgggtttaac aacggattcc ccatccagaa
    25021 tgccagcgca ccgcccatcg acacctgttg gcgacgcatt cccgccgcga ccggagccgc
    25081 acagcaggta cacatcatgc ccggcagcga aaacagcgtt cccagcagcg tgccgcgaaa
    25141 gcgcgattgc ccaagcgtac gcaacaacca gtcacgcggg atcagcacct gaatcaacga
    25201 accgaggatc ccccccagca ccgccgcttt ccataccgcg agaaaataga tcatcgcgta
    25261 atccaacgcc gcctgccacg gattagcatc cgcctgcgca aggatagatt taccgatact
    25321 gtgggtttcg gcagcagtaa acgctttgcc gtagtaaggt tgccatttca cataccagag
    25381 accggcaatg acaacgagaa agaaaagcgc gggtttccac cactgaatgg gcgttgccgc
    25441 ctgagatgaa gactgaccag tcatagcatt ccccaggaat aagttatgat aattgagcgc
    25501 gtgaatatta cgctcactat caattcttgg gaataattat tcagctcttt tgcgtaattc
    25561 tgaagagctt aaaatcgtca caccttcatg ctcaggccgc atcgcctctg ccagcatgac
    25621 ccgcgccacg tctctcgcat caatggattt ccagttaccg ggtaacaaac ggaacagcgg
    25681 cgcaaaaagc gtttcgttca tccgctgttt gctacgatcg cccagtaaca tcgacgggcg
    25741 agcaatggtc aatttcggcc agttctgggc gattaatgct tcttccatct cccctttgac
    25801 gcggttatag aaaaacggcg agtgggcatt agcacccata gcactgacca ccagcatatg
    25861 ctgcgcaccg agccgccgcc cggttaatgc ggtatccact accaacgtgt aatcggcatg
    25921 aataaacgcc tctttgctcc ccgcttcccg ccgcgtggtg ccgagacaac aaaatacaat
    25981 gtcgatggga tcggtgacct gcgccagcgc gtcggtcagt tgcgggtcat ggggattaaa
    26041 cacccctggc atatcgccca acgggcgtcg cgtcggcgcg gctatggcgt taactttcgg
    26101 ttcgttaatc aacatccgca gcaggtgacc gcccaccagc cccgttgcgc ctgtaatcag
    26161 tacctgactc atcttcgctc ctttacagaa ttgtctgcct tgcgctcccc ggctcatgca
    26221 ccaggcttaa tagtctacga ggtaattatc cccgatcgtg agaaattcgc ctcatccaat
    26281 gcaacaacgc ggaggaaaca tgagtaagaa gattgccgtt ttaatcactg atgaatttga
    26341 ggattcagaa tttacttcac ccgcagacga gttccgtaaa gccggacacg aagtgattac
    26401 cattgaaaaa caagcgggta aaacggtgaa aggcaaaaaa ggagaagcca gcgtgaccat
    26461 cgataaatcc atcgatgaag tgacgccagc ggagtttgat gccctgctgc taccgggcgg
    26521 tcattcacca gattatctgc gtggtgacaa ccgttttgtc accttcacac gcgattttgt
    26581 gaacagcggc aaacctgttt tcgccatctg tcacggtccc cagttgctga tcagcgccga
    26641 tgtgatccgt ggtcgtaagc tgaccgccgt taaaccgatc gtgattgatg ttaaaaacgc
    26701 gggcgcggaa ttttacgatc aggaagtggt ggtcgataaa gatcagctgg ttaccagccg
    26761 gacaccggac gatctgccag cgtttaaccg cgaggcgtta cgcctgctcg gtgcctgagt
    26821 tacgtagcca aatcattttt ttaccaaagc ccagcgtgtt gtcggtgaat ttgatttcat
    26881 cgagccggat ttcccatacc ggggccgaca gcattctggc aaccggaaag cgacgattgt
    26941 acgccttgcg tgcgaggtcg ctttcctcgc cttccagtct gcggatctca cctttaaact
    27001 gcacgccgcg aattaacgct accgttttcg gctgaccgtt taccgttccg gcaattgccg
    27061 cctgcggccc gctcatctgc gcatgacgcg ttttttcttc cgtcaaaatg tagaaagcga
    27121 ctttctgcgc atcaaaaaga taaaaggcat tggcgcacca cagttcccct tcctgctgca
    27181 cacaccaggt gacaacatgt tgttttgcca gccaacggct gatggcggtg agtgtttcca
    27241 ttgctgttct cttttatact gtgggcctga ccttaacatg cgacgcagac gatgacaccc
    27301 tggtttcttt acttgatccg taccgccgac aataagcttt ataccgggat caccacggat
    27361 gtcgaacgcc gctatcagca gcaccaaagc ggcaaagggg cgaaagcact gcgcgggaag
    27421 ggagaactaa cgctggcgtt ttccgcacca gtgggcgatc gttcgctggc attacgggca
    27481 gaatatcgcg ttaagcaact gaccaaacgg caaaaagagc gtctggtagc ggaaggtgca
    27541 gtgtttgcag aactgttgaa cagcctgcaa accccggaga ttaaaagcga ttgaagtgct
    27601 cgtgatactc aaccagacca gttacaccgt tcagcgcgtc atccgctaaa cgatgtacct
    27661 ggaaggcact ttcagtgccc ggccagcggc aacgcagatc gtgatgtgcc gccagttcaa
    27721 agccgaaacg gctgtacagc gccggatcgc ccagcgtcac cactgcggca tagccgaact
    27781 cattaagcga atcgagtcct tcatagacca gttggcgtgc cagcccctgt ccacggtatt
    27841 tttcatctac cgccaacggt gccatgccga cccattgcag gtcttcgccc tgcacatcaa
    27901 ccggactaaa tgccacatag ccaatgacct gaccttcgtc atctgtcgcc accagcccca
    27961 gcgtcagaaa gccatcttca cgcagatcgt gaaccagctt cgcttccgca tcactttcga
    28021 atgaacgacg cagcagggca tcaatacccg gcgcatcaat gggaatttct actcgaatta
    28081 gcatggttca cctaccgatg tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac
    28141 aaaatccgcc agttgcagca gcatcatgcg taaggctttc ggcatttgct ccagttcaat
    28201 ggcgtccatc aggtttttca catacagccc cagctccgta tcgccttcaa tcaccagccg
    28261 acgctggaag aagagcgtat ccggatcttg tttacgcgcc gcaatcatca gcagatcgct
    28321 ggcgtcggca ctaaaactca catcagcttg cgcgttctgg ctaacgacca gtttgccatt
    28381 caccaccgag gtaaaccatt gcaggtcaat atcacgcaca tgaatactta accagcgacc
    28441 ttcaagaaac tccagctcgc catcatccag cgcctgacgg aattgccagc ttaagacctg
    28501 ctcaagaacc tggcgtttta gcgcaaatgg cgtcagtttt accggtacac tcaacagaga
    28561 tggccccaaa tgcacaatac gggaacgcag tttatccaac acgagtttta ctccctgttt
    28621 caacaatcat cctattttgc catatcagaa aaataacata gcggtataaa tcaacaattc
    28681 catatgaaat tgctgctacc accaatacaa ctttaactgc cttaaatcaa aaattgtcgc
    28741 agcaaggtta actaaaatcc cagttcgtta acatttttgc gctttgatag cgcaaccttc
    28801 aggaaaaatt atggagctgc tctgccctgc cggaaatctc ccggcgctta aggcggccat
    28861 cgaaaacggc gcagatgctg tttatatcgg gctaaaagat gataccaatg cccgtcactt
    28921 cgccggcctt aactttaccg agaaaaaatt gcaggaagcg gtgagttttg tccatcaaca
    28981 tcgccgcaaa cttcacatcg cgattaacac ttttgcgcat ccggacggtt acgcccgctg
    29041 gcagcgcgcc gtggatatgg cggcgcagct gggtgccgac gcgctgatcc tcgccgacct
    29101 cgccatgctg gagtacgccg ccgagcgtta cccgcatatt gagcgtcatg tgtcagtgca
    29161 ggcttcggcg accaatgaag aggcgattaa cttttatcat cgccattttg acgttgcccg
    29221 cgtggtgctg ccgcgcgtgt tgtcgattca tcaggtgaaa caactggcac gggtcacacc
    29281 tgtaccactg gaagtctttg ctttcggcag cctgtgcatt atgtcggaag gtcgttgcta
    29341 tctgtcgtcg tatttgacgg gtgagtctcc caacaccgtg ggcgcgtgtt ctccggcccg
    29401 tttcgtgcgc tggcagcaaa cgccgcaggg gctggaatcc cgcctgaacg aagtgctgat
    29461 cgaccgttat caggacggcg aaaacgcagg ttatccgacg ctgtgtaaag ggcgttatct
    29521 ggtggacggc gagcgctatc acgcgctgga agaacccacc agtctcaata ccctggaact
    29581 gctgccggag ttaatggcag cgaatattgc ttcggtgaaa attgaaggcc gccagcgtag
    29641 cccggcgtat gtcagccagg tggcgaaagt ctggcgtcag gctatcgacc gttgtaaggc
    29701 cgatccgcaa aacttcatac cgcaaagcgc gtggatggag acgctcgggt cgatgtccga
    29761 aggcacgcag accacccttg gcgcgtatca ccgtaaatgg cagtgagaaa agcaatgaaa
    29821 tattccttag ggccagtgct gtggtactgg ccaaaagaga cgctggagga attttatcag
    29881 caggccgccg ccagcagcgc cgacgtgatt tatcttggtg aagcggtatg cagcaagcgc
    29941 cgggcaacca aagttggcga ctggctggag atggcaaaat cactcgccgg aagtggtaag
    30001 cagattgtgc tctccacgct ggcgctggtg caggcatcct ctgaactggg cgaactgaaa
    30061 cgctatgttg agaacggtga gtttctgatt gaagccagcg atctcggcgt ggtgaatatg
    30121 tgcgctgaac gcaaactgcc gttcgtcgcc ggacacgcgc taaactgcta caacgcggtg
    30181 acgctgaaaa tattgctcaa acagggcatg atgcgctggt gtatgccggt ggagctttcc
    30241 cgcgactggc tggtgaatct acttaatcag tgcgatgaac tgggtattcg caaccagttt
    30301 gaagtggaag tcttgagcta cggtcatctg ccgctggcct actccgcccg ctgctttacc
    30361 gcgcgttcgg aagaccgccc gaaagatgag tgtgaaacct gttgcattaa gtatccgaac
    30421 gggcgcaacg tgctgtcgca ggagaatcag caagtatttg tactcaacgg cattcagacc
    30481 atgagcggct acgtgtacaa cctcggtaac gagctggcat ccatgcaggg gctggttgat
    30541 gtggttcgtc tgtcaccgca ggatactgac actttcgcga tgctcgacgc cttccgcgcc
    30601 aatgaaaatg gcgctgcgcc actgccgttg acggcaaaca gcgattgcaa cggctactgg
    30661 cggcggctgg ccgggctgga gctgcaagca taagtatata gctcactttg ttaacaactt
    30721 taactactct ttaatgcagt attaaagatt aatcggtaac aaagtgagct gttatgactg
    30781 ataaaaccat tccgttttcg ctactcgatc tggcccctat tcccgaaggt tcttcagcgc
    30841 gagaagcgtt ctcccactct ctcgacctcg cccgtctggc tgaaaagcgc ggctatcatc
    30901 gctactggct ggctgaacac cacaatatga ccggcattgc cagtgctgca acatcagttt
    30961 tgattggcta tctggcggcg aataccacta cgctgcatct ggggtctggc ggtgtgatgt
    31021 tgcctaatca ctcaccattg gtgatcgccg aacagttcgg cacgcttaat acgctctatc
    31081 cggggcgaat cgatttgggg ctgggtcgtg ctccgggtag tgaccagcga accatgatgg
    31141 ctctacgtcg tcatatgagc ggcgatattg ataattttcc ccgcgatgtc gcggagctgg
    31201 tggactggtt tgacgcccgc gatcccaatc cgcatgtgcg cccggtacca ggctatggcg
    31261 agaaaatccc cgtgtggttg ttaggctcca gcctttacag cgcgcaactg gcggcgcagc
    31321 ttggtctgcc gtttgcgttt gcctcacact tcgcgccgga tatgttgttc caggcgctgc
    31381 atctttatcg cagcaacttc aagccgtcgg cacgactgga aaaaccatac gcgatggtgt
    31441 gcatcaatat tatcgccgcc gacagcaacc gcgacgctga atttctgttt acctcgatgc
    31501 agcaagcctt tgtgaagctg cgccgtggcg aaaccgggca actgccgccg ccgattcaaa
    31561 atatggatca gttctggtca ccgtccgagc agtatggcgt gcagcaggcg ctgagtatgt
    31621 cgctggtggg tgataaagcg aaagtgcgtc atggtttgca gtcgatcctg cgcgaaaccg
    31681 acgccgatga gattatggtt aacgggcaga ttttcgacca ccaggcacgc ctgcattcgt
    31741 ttgagctggc gatggatgtg aaggaagagt tgttggggta gtgtgtttta acgtaggaag
    31801 ccttatccga gatggcaacg ctgtcctacg taggcctgat aagcgaagcg catcaggcat
    31861 tgtgtaggca gcagaaatgt cggataaggc accgctgatt actgatacac cggcagcaca
    31921 ttaaagctcg ataagatatg gaccagtgcg ttgccaacgc caaataccag aatcagcata
    31981 atcatcggct tgccgcccca tacacggaat ttcgggctac caaagcgttt acgcgatgcg
    32041 cgggccaaca gcgccggaac aattgccgcc cagatggtag ccgctaagcc agcataacca
    32101 atggcgtaca ggaatccgtt aggaaacagc aggccaccca caacaggcgg ggcaaaggtc
    32161 agcaacgctg ttttcaaacg gcccatagcc gagtcgtcaa aaccaaacag atctgccaga
    32221 tagtcaaaca aacccagcgt tacgccaagg aacgaactcg ctaccgcaaa gtttgagaac
    32281 acgaccagta gaaggtccag actacggctg ttcagtacgc cgcttaacgc ctgtaccagc
    32341 acatcaatat taccgccctt ctcggcgatg ccgataaact ccggacgcgg gatgttaccc
    32401 atcgtcgcca gcaaccagat ggtatacagc gccagcgcca tcagcgtacc gtacaccagg
    32461 catttcacga tggttttcgg atctttgccg taatacttca tcaggctcgg tacgttaccg
    32521 tgataaccaa acgatgccag acagaacggc agcgtcatca acagatacgg tgcataagac
    32581 gcattgcttt cggcgacgtt gaacaatgtc gcaggctgca catgccccag caggctaccg
    32641 aaggtgagga agaaggtaat gactttcgcc cccagcacaa tcgctgtcat gcgactgacg
    32701 gctttggtgc tcaaccacac cacaaacgct accaacagtg caaaaccaaa acccgctgcc
    32761 cgtgccggga cgtttagcga catctctgcg aaggtgtgat gcagaatcga accgctggca
    32821 gaaatatagg cgtaagtcag gatatagagc acaaaggcaa tggaaatgcc gttgactacg
    32881 ttccagcctt tgcccagcaa atctttggtg atggtgtcaa agctagaacc gattcgataa
    32941 ttcaggttag cttccagaat catcaagccg gaatgcagca tacagaacca ggtaaagatc
    33001 agcgccgcca ttgaccagaa gaaccacgcc ccagacatga ccacaggcag agaaaacatc
    33061 cctgcgccaa taatggtgcc gccgataatc accacgccgc caagcagcga cggtgacgtt
    33121 tgggtggtgg ttagtgttgc catgagggct tctctccagt gaaaaatagt gcgactgtgt
    33181 tgttatgcat tgcactgtac cagtacatga gtacaaaaaa cagaaaaaaa gccccgatgg
    33241 ttaaaatcgg ggctgtatat attattttac agattgtgtt cgctgttcag cgatgattac
    33301 gcatcaccac cgaaacgacg acgaccggta gagtcatcac gacgcggtgc gcggccttca
    33361 cgacgttcgc cgctaaaacg acgcccatca ccacgacccc cttcacggcg ttcaccgctg
    33421 aagttacgac cgccttcacg acgttcgcca ccgaaaccac gaccaccgcc acgacgctca
    33481 ccgccagtat gcggctgtgc atcgcccagt aactgcatgt tcatcggctt gttgagaatg
    33541 cgagtacgcg taaagtgttg cagcacttca cccggcatac ctttcggcag ttcgatggtg
    33601 gagtgagaag caaacagctt gatgttaccg atgtaacggc tgctgatgtc gccttcgtta
    33661 gcaatcgcac caacgatatg acgaacttca acaccatcat cgcggcccac ttcaatacgg
    33721 tacaactgca tatcgccaac atcacgacgt tcgcgacgcg gacgatcttc acggtcgcca
    33781 cgcgggccac ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg gaattcacgt
    33841 ttcggacgca tcggcgcatc tggcggaaca atcagagtac gttcaccctg tgccattttc
    33901 agtagtgcgg ctgccagagt ttcgagatcc agctcttcac cttcggcagt cggctgaatt
    33961 ttgctcagca gtgcacggta ttgatccaga tcgctgcttt ccagctgctg ctgtacttta
    34021 gcggcaaatt tttccagacg acgtttgccc agcagttctg cgttcggcag ttctacttcc
    34081 ggaatggtca gcttcatggt gcgttcaatg ttacgcagca gacgacgctc acggttctca
    34141 acgaacagca gtgcacgacc agcacgacct gcacgaccgg tacgaccgat acggtgaacg
    34201 taagactcag aatccatcgg gatatcgtag ttaaccacca ggctgatacg ctcaacgtcc
    34261 aggccacggg ctgcaacgtc ggtcgcaatc aggatgtcca gacgaccatc tttcaggcgt
    34321 tccagtgtct gctcacgcag cgcctggttc atgtcaccgt tcagcgcggc gctgttgtag
    34381 ccgttacgct caagagcttc tgccacttcc agagtcgcgt ttttggtacg aacgaagata
    34441 atcgccgcat caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt tttacgcata
    34501 ccccagacag tccagtagct ctggctgatg tcaggacggg tagtcacgct ggactgaatg
    34561 cgcacttcct gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc ttccggcatg
    34621 gttgcagaga acagagcggt ctgatgacct tccgggatct gcgccataat ggtttcaacg
    34681 tcttcgatga agcccatgcg cagcatttcg tcagcttcat ccagaaccag accgctcagt
    34741 ttagagaggt ccagagtgtc acgtttcagg tggtccagca gacggcccgg agtaccgaca
    34801 acgatctgcg gcccctgacg cagggcgcgt aattgcacgt cataacgttg gccgccgtac
    34861 agggcaacca cgtttacgcc gcgcatgtgt ttagagaaat ccgtcattgc ttcagcaacc
    34921 tgtaccgcca gttcgcgggt cggtgccagc accagaatct gtggtgcttt cagctcagga
    34981 tcaagattct gcagcagagg tagagagaat gctgcagttt ttccgctccc cgtctgggcc
    35041 atacccagaa cgtcgcggcc attcagcaga tgtggaatac actctgcctg aattggagat
    35101 ggtttttcgt aacccagatc gttaagggct tcaaggatag gagccttcag gcccagatct
    35161 gcaaaagtgg tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa tggcggccag
    35221 tctacataac tcatcatgaa attgatcagc aattttcatt gaaaagtgtg aaccggctca
    35281 aagtaggtgt attaacgaac aacaacgccc tcacccgtta aggtgatggc aatcaaaaaa
    35341 gattacgggc tgatgtgtac gtcagctatt gctggtccga ttctgccagg tcatcttggt
    35401 cctggcccag gagcgataat tccaacaatg cgtatcggtg ctcaacaaag ttatgaacgt
    35461 tgttggcaac cgccagtttg aacagtgccg tggcgctgtc caaatccccc agacttaggt
    35521 agtacttacc taaatagaag ttggtttcac tgagatgctc agcgagcgag gtgttatccg
    35581 ttgcgtccgc cttgagcctt tccattaacg tttgttcgct aatgttgccc aggtagaact
    35641 cgacaatgtt ccatccccac tgttccttat ccgatttttc gaagtgctgt ttcaacactt
    35701 ctttagcctg cttctcatcg agcttctgct cggcgagata aagccacaga ctacggaaag
    35761 gatcattggg atcgtcttga taaaacgcca gcagatcatc ttgcgctaac ttgtcacgac
    35821 cgccgtaata taatgcgatc ccgcgattca agtgcgcgta gttgtaagtt ggatcaagct
    35881 caagtacaga atcaaacgct tcataggcag catcaaaatt gcctgcctgc gttaaatata
    35941 tgcctaagta attgaatact tcaggcatat ccggtcggat tgccagcgct tgcgaaaaat
    36001 cgttacgcgc taatgccctc agaccgagac tatcatacaa cactccgcgc tcatataaaa
    36061 gctgtgcgcg ttcgtcatcg gttaaagccc gactggcaag gatctgttct atacgtgcca
    36121 gaatcacttc ctgctgtaaa gtcggttgca atggtaccgc gaggacttca cttttacgcc
    36181 aggaagtatt actgcatcct gcaagcgtaa gtgctgtcgc aacgaaacac cagcgcaaaa
    36241 aaggcttcat ttcccactcc cgaagaccac ggttgaatga acgtcctgtt cccggttgct
    36301 aacaaggcgt cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa tggcaacctt
    36361 actcgccctg ttcagcagcc ggagcttccg gtgctgcagc aggttgagat tgctcagtcg
    36421 cttctttaat gctcagacgg atacggccct ggcgatcaac ttccagaact ttcaccggta
    36481 cttcctgacc catctgcagg taatcggtca ctttctcaac gcgtttatca gcgatttgag
    36541 agatgtggac cagaccttct ttaccgccac caatggcaac aaatgcgcca aaatcaacga
    36601 tacgggtcac tttaccattg tagacgcggc ccacttcgat ttctgcagtg atctcttcga
    36661 tacgacgaat cgcgtgtttc gctttctcgc cgtcggtcgc tgcaatcttc acagtaccgt
    36721 catcttcaat ttcgatggtg gtgccggttt cttcggtcag agcacggatt acagaaccgc
    36781 ctttaccgat aacatctttg atcttgtccg ggttgatctt gatggtatgg atacgcggtg
    36841 cgaactcaga gatatcgcca cgcggcgcgt tgatcgcctg ttccattacc cccaggatat
    36901 gcagacgcgc acctttagcc tggttcagcg caacctgcat gatctctttg gtgatacctt
    36961 caattttgat atccatctgc aatgcagaga taccgtcacg ggaacccgca actttgaagt
    37021 ccatatcgcc caggtgatct tcgtcgccca aaatgtcaga cagtacaacg tagttgtcgc
    37081 cttctttcac cagacccatt gcgatacccg caacggcagc tttgattggc acacctgcgt
    37141 ccatcagcgc cagagacgcg ccgcacacgg aagccataga agaggaaccg ttggattcgg
    37201 tgatttcaga cacaacacgt acggtgtacg ggaatttgtc catatccggc attactgcca
    37261 gcacgccgcg cttcgccaga cgaccgtgac caatttcacg acgcttcgga gaaccgacca
    37321 tgccggtttc gcctacggag tacggaggga agttgtagtg gaacaggaag gtatcggtac
    37381 gttcgcccat cagttcatca agaacctgcg cgtcacgagc agtacccagc gttgcggtaa
    37441 ccagcgcctg cgtttcacca cgggtgaaca gcgcagaacc gtgagtacgc ggcagcacac
    37501 cagtacgcac atccagacca cggatcatat ctttttcacg accgtcgata cgcggttcgc
    37561 ctgccagtac tcggctacga acaacatttt tctcgatagc gtgcagaatt tcacccagtt
    37621 cgttttcgtc cagggtttcg tcttcagcaa gcagtgtcgc gatggtttca gatttgatga
    37681 catcaacctg cgcataacgc tcttgtttgt cggtgatgcg gtaagcatcg ctcagacgag
    37741 cttcagccag tgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc ggctgccagt
    37801 cccaacgcgg tttaccggct tctttcacca gttcattgat gttctgaata acaacctgct
    37861 gttgttcatg accgaacacc actgcgccca gcatttggtc ttcgctcagc agttcagctt
    37921 cagattcaac catcagcacg gcagcttctg taccggcaac aaccaggtcc agtttgctct
    37981 ctttcagttc gtcctgagtc gggttcagta cgtactggtc attgatgtaa cctacacggg
    38041 cagcaccaat cgggccattg aacggaatac cagacagaga cagtgctgcg gaagcaccaa
    38101 tcatcgcgac gatatccggg ttaacctgcg ggttaacaga aaccacggtg gcgataacct
    38161 gaacttcgtt gacgaagcct tccgggaaca gcgggcgaat cgggcggtca atcagacgcg
    38221 cgatcagggt ttcgccttcg cttgggcggc cttcacgacg gaagaagcta cccgggatac
    38281 gaccagcagc gtaggtacgc tcctgatagt taacggtcag cgggaagaag tcctgacctg
    38341 gtttggcttt tttctgacca acaacggtaa caaataccgc ggtgtcatcc atgctaacca
    38401 taacagcggc agtagcctga cgagccatca tgccggtttc cagagtcacg gtgtgttggc
    38461 cgtactggaa tttacgaacg atcggattaa gcaatgtaat atcctttctc tttcttagac
    38521 agtaccttac ggtactggtg ttaatacccg atcttctgcg catcctcgcg actaatgaca
    38581 accctaaccc agctctatgt gggtaaagcc tctcattagc cgcgcgaacc tctgcaacgg
    38641 aagatcattc atagcaacaa tacattagtt tccagtgaat tgctgccgtc agcttgaaaa
    38701 aaggggccac tcaggccccc ttttctgaaa ctcgcaagaa ttagcgacgc agacccaggc
    38761 gctcgatgag ctgggtgtaa cgtgctacgt ctttacgttt caggtagtcg agcagtttac
    38821 gacgctgaga aaccatgcgc agcagaccac gacggctgtg gtgatctttt ttgtgctctg
    38881 caaagtggcc ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga acttcggtag
    38941 aaccggtgtc gtttgcgtca cgaccaaact cagaaacgat tttagctgtt gcttcagtac
    39001 ttagagacat tttaaaactc caaagtatat agaatgaaag gacgccgatc tctaattcag
    39061 cgatcccagt gtacgttacg caaagtgtta aacaatttac gcgacgttaa gcggcagtat
    39121 tctactcgta gcgacctgtt atcgcaagac ggttaacatt acgccgggta ttcaaccacc
    39181 aggcgacgag gcgcaacgcg gccttcatcg tcaatttcgc ccataccgat aaacttgccg
    39241 ttctcaccct ctgtaacgcg aaccagtcct tccagcggcg caccggatgt acgaactggg
    39301 ttaccatttt tgaagtaaac agaagacgtt aacggaagat tcaccactgg gtagtccgaa
    39361 gctggactgt ccattggcat cagtaatgga tcaagtaact ccgcagccgg aatatcctgc
    39421 tgttcggctt gctcaaccag ttcacgcaga tgttccaggg tcaccatccg ttcaaccgga
    39481 tatttactta ccgccagacg acgcaagtaa ataacatgcg cgccacaacc gagtttttcg
    39541 ccgagatcgt caatgatagt gcggatataa gtgccttttg agcagtgaat ttccagctcc
    39601 agctcattgc cttcatggcg aataaacagc aattcataaa cggtaatcgg gcgcgcttca
    39661 cgcggaactt caatgccctg acgcgcgtat tcgtacagtt ttttgccctg atatttcagt
    39721 gctgaataca tcgaagggat ctgttcgata tcgccacgga aggtatccag cgccgccgcc
    39781 agttgctctg cactaaaggt taccggacgt tcttcaacga tttgtccgtc ggcatcagaa
    39841 gtatcggtac gctgtccaag acgcgcaatg acccgatagc gtttgtcgga gtccagcaga
    39901 tactgggaaa acttcgtcgc ttccccgagg caaattggca acatgccggt cgccagcggg
    39961 tccagcgcac cggtatgccc ggcacggttg gcgttataaa tccgtttcac tttttgcagc
    40021 gcatcgttgc tggacatccc ctgcggttta tccagcaaca aaacgccgtt aatgtcgcga
    40081 ccgcgacgac gaggacgact cattagtcct ccttgctgtc gtccgggtta acacgacgtt
    40141 cttcgtcatg tttgaccacg ctggtcacca ggtttgacat gcgcatccct tcaaccagag
    40201 agttgtcgta gaagaaggtc agttccggca cgatacgcag gcgcatcgct ttccccagca
    40261 ggctgcggat gaaaccagaa gcttcctgca acgctttgat gcccgcttta accgcgtctt
    40321 catctttgtc gttgaggaac gtcacatata ctttggcata cgccaggtcg cgagacattt
    40381 cgacaccgga aacggtggtc atcatgccca gacgaggatc tttaatttca cgctgcagga
    40441 tgagagcgat ctctttttgc atttcctgcg ctacgcgctg cgggcgacca aattctttcg
    40501 ccataataaa ttctcctgac aaaaaagggg ctgttagccc ctttttaaaa ttaatttcag
    40561 gtggaagggc tgttcacgtt ggcctgataa gacgcgccag cgtcgcatca ggcaatccat
    40621 gcaggatgcg acataaacgc cttatcccgc atggaaccct aaaaacctta agcgatagta
    40681 cgttggatct cgatgatttc gaatacttcg atcacatcgc cagtgcggac atcgttgtag
    40741 ttcttaacgc cgataccaca ttccataccg ttacggactt cgttaacgtc atctttgaag
    40801 cgtcgcaggg actccagctc gccttcgtag ataaccacgt tgtcgcgcag aacgcggatc
    40861 gggttgtgac gtttaaccac accttcggta accatacaac ctgcgatagc accaaatttc
    40921 ggtgatttga acacgtcacg aacttccgcc agaccgataa tctgctgttt cagttccgga
    40981 gacagcatac cgctcatcgc cgctttcact tcgtcgatca ggttatagat gacggagtag
    41041 taacgcagat ccaggctttc cgcttcaatc actttacgtg cagaggcatc agcacgtacg
    41101 ttgaagccaa ccaggatggc gttggacgcc gcagccaggg tagcgtcggt ttcggtgata
    41161 ccacctacgc cagaaccgat gatcttcact ttaacttcgt cagtagacag tttcagcaag
    41221 gagtcggaaa ttgcttccac agaaccctgt acgtctgcct tcaggacaat attcacttcg
    41281 tgaacttcgc cttcggtcat gttggcgaac atgttctcga gtttagattt ctgctgacgc
    41341 gccagtttaa cttcgcggaa tttaccctga cgatagagtg caacttcacg cgctttcttc
    41401 tcgtcacgta caacggtaac ttcatcaccc gcagccggta cgccggacag gccaaggatt
    41461 tccaccggaa tggacggacc cgcttccagc acttcctgac ccagttcgtt acgcattgca
    41521 cgaacacgac cgtattcgaa gccacacaga acgatatcgc ccttgtgcag agtaccttca
    41581 cgtaccagaa cggtagcaac cggaccacga cctttatcga ggaaggattc gataaccgca
    41641 ccgctcgcca tacctttacg taccgctttc agctccagaa cttccgcctg cagcaggata
    41701 gcatccagca gttcatcaat accggtaccc gcttttgcag atacgtgtac gaactggctt
    41761 tcgccgcccc actcttctgg caggatgccg tactgggaga gttcgttctt aacgcgatcc
    41821 ggatcggctt ctggtttatc gattttgttc acagcaacca ccaccggaac acccgccgct
    41881 ttcgcgtgct ggatagcttc gatggtctgc ggcattacac cgtcgtctgc agcaacaacc
    41941 aggactacga tgtccgttgc ctgcgcacca cgtgcacgca ttgaagtaaa cgcggcgtgc
    42001 cccggggtgt ccaggaaggt gatcatgccg ttttcagttt caacgtggta tgcaccaatg
    42061 tgctgggtaa tgccgcccgc ttcgccagag gccactttcg ttgaacgaat gtagtccagc
    42121 agagaggttt taccgtggtc aacgtgaccc atgatggtca caaccggcgc gcgcggttca
    42181 gccgcagcac ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt ttcacgacgc
    42241 aggataactt tgtggcccat ctcttcagca accagctgtg cggtttcctg atcgataacc
    42301 tggttgatgg ttgccattgc gcccagtttc atcatcgctt tgatgacctg agagccttta
    42361 accgccatct tgttcgccag ttcgccaacg gtgatggttt cgccgatcac aacgtcacgg
    42421 ttaacggcct gagctggctt ctggaagcct tgctgcagcg aagaaccttt acgttttccg
    42481 cccttaccgc cacgtactgc tgcgcgagct tcttcacgat cagcttttga ttcagcgtgt
    42541 ttgttgccct tcttcggacg cgctgctttc gcgttacgac cacggccacg gccgccttcg
    42601 acttcacgat cgctttcgtc ttctgcctgg cgagcatgtt gagaagtagt gacgtgataa
    42661 tcgctggaat cttcagtcgg ttctgcgtta tcagtccatt tgttttcttc cgccatacga
    42721 cgtgcttctt cagcaacgcg acgtgcttct tcttcgagtt tacgacgcgc ttcttcttcg
    42781 gctttacgct tgagctctgc agcttcctgc tcacggcggg ctttttcagc ctgggcgttt
    42841 ttagtcatat cgtcttgttg attgctcact ttgtcttttt ccgcagcttc acgtttcgct
    42901 tgttcagcag cttcacgctt agcttgttct gcggcctcac gttcagcttt ttgttgcgcc
    42961 tcacgtttgg ccgattcttc tgcctcacga cgggcttgct cttccgcttc acgctgcgct
    43021 tgctcttccg cagcaaggcg ttcagcctct tgcggatcgc gtttcacaaa ggtgcgtttc
    43081 ttgcggactt cgatttgtac cgatttgctt tttccaccgg taccaggaat gttaagggtg
    43141 ctgcgtgttt tacgttgcag cgtcaatttg tccgggcctg aatttttctg attcaggtgg
    43201 tcaatcaaag tctgtttctc ttgtgcagac acagagtcgt cagcagactt ccggatacct
    43261 gcatcagcaa attgctgtac caggcgttcc acggaggtct gtcgctctgc ggccagcgtt
    43321 ttaatcgtta catctgtcat gctgttcctt cctgctacag tttattacgc ttcgtcaccg
    43381 aaccagcaaa tattacgggc agccataatc agtgctccgg ctttttcgtc ggtcaaccct
    43441 tcgatatcag ccagatcatc aatgccctgt tcggcgagat cttccagcgt acaaacgcca
    43501 cgggcggcca gtttgaatgc caaatcacga tctacccctt caaggttcag cagatcgtca
    43561 gccggtttgt tatcaccgag gctttcttcc tgggcctgtg caatggtggc cagtgcattt
    43621 ttagcacgct cgcgcagtgc ttcaacggtc ggctcatcaa ggccttcgat ttccaacagc
    43681 tctttcatcg gcacataggc cagttcttcc agcgtcgaga agccttcttc taccagaaca
    43741 gtcgcgaagt cttcatcgat atcgagatat ttggtgaagg tgtcgatcgc tgcgtgcgct
    43801 tccgcctgat gcttagcctg caggtcgtca acggtcatca cgttgagttc ccaaccgctc
    43861 agctgcgaag ccagacgcac gttctgaccg ttacggccaa tcgcctgcgc caggttaccg
    43921 gcttcaacgg cgatatccat ggtgtgttta tcttcatcca ccacgataga agcaacgtct
    43981 gccggtgcca ttgcgttaat cacaaactgc gccgggttat catcccacag gacaatatcg
    44041 atacgctcgc cgcccagttc agtagaaacc gcctgaacac gcgcgccacg catacctacg
    44101 caagcaccta ccggatcgat acgtttatcg ttggttttca ccgcaatttt cgcacgagat
    44161 cccgggtcgc gagccgctgc tttaatttca atcacttctt cgccgatttc cggcacttca
    44221 atacggaaca gttcgatcag catttccggc ttggagcggg tgacgaacag ctgcgcgcca
    44281 cgcgcttccg ggcgaacgga atagagcacg ccacgaacgc ggtcgccagg gcggaagttt
    44341 tcacgcggca gcatatcttc gcgcaggatc acggcttcag cgttgttgcc cagatccaga
    44401 gagatgttgt cgcggtttac ttttttcacc acgccggtga tgatttcacc ttcgtgttca
    44461 cggaactgat caaccaccat tgcacgttcg gcttcacgca ctttctgcac gataacctgt
    44521 tttgccgtct gggtagtgat acggtcaaag gtaacagact caatctgatc ttcaacgtaa
    44581 tcgcccaggt tcaggctttc atcttcataa cgtgcggctt caagggtgat ttccttggtc
    44641 ggctgggtga cttcatcaac aactaaccag cgacggaagg tgtcaaaatc accgcttttg
    44701 cgatcgatct gtacgcgaac gtcgatttct tgttcatatt ttttctttgt tgctgtcgcc
    44761 agcgcgcttt ccaatgcttc gaaaatcttc tcgcgaggta gcgccttttc attggatacg
    44821 gcttcaacta cagccaaaat ttctttgttc atcgcgggct tttcacctca tccagactat
    44881 taaaagtggg gaaccaggtt cgccttctgg atattactca gcgcgaacac ttcatcttta
    44941 ccttcgacgg taactgtgat catttcaccg tctaccgctt tgataacgcc ctgccattta
    45001 cgacggtttt gtaccgccat acggagaacc agagtcacct cttctccgac aaaacgggcg
    45061 tagtgttcag ccgtgaacag tgggcgatcg agacccggtg aggagacttc caggttataa
    45121 gcaacggtga tggggtcttc aacatccagc acagcactta cctggtggct cacatcagca
    45181 caatcatcaa cattgatgcc atcttcacta tcaatataga tgcgcagtgt ggatgtgcga
    45241 ccgcgaataa attcgatgcc aaccagttca aaacccaggg cctcaactgg cgcagtaatc
    45301 atctctgtta atttttgctc taatgtggac aagcccaccc ccaagacata aaaaaagggc
    45361 ctaaagccca gttattctgt agtcagataa caaaaaaccc cgataaatcg gggctttata
    45421 taactgaacc ctataaccgc aactgcggtc tggagcactt tccagaagga ttttttcaaa
    45481 tcccactacg aaggccgaag tcttcacagt atatttgaaa aaggactcta agggaaagtg
    45541 gttgcggggg ccggatttga accgacgacc ttcgggttat gagcccgacg agctaccagg
    45601 ctgctccacc ccgcgcctga aacgtggcaa attctactcg ttttgggtaa aaaatgcaaa
    45661 tactgctggg atttggtgta ccgagacggg acgtaaaatc tgcaggcatt atagtgatcc
    45721 acgccacatt ttgtcaacgt ttattgctaa tcacgtgaat gaatatccag ttcactttca
    45781 tttgttgaat acttttacct tctcctgctt ttccttaagc gcattatttt acaaaaaaca
    45841 cactaaactc ttcctgtctc cgataaaaga tgattaaatg aaaactcatt tattttgcat
    45901 aaaaattcag tgagagcaga aatccaggct catcatcagt taattaagca gggtgttatt
    45961 ttatgacgac gattctcaag catctcccgg taggtcaacg tattggtatc gctttttccg
    46021 gcggtctgga caccagtgcc gcactgctgt ggatgcgaca aaagggagct gttccttatg
    46081 catatactgc aaacctgggc cagccagacg aagaggatta tgatgcgatc cctcgtcgtg
    46141 ccatggaata cggcgcggag aacgcacgtc tgatcgactg ccgcaaacaa ctggtggccg
    46201 aaggtattgc cgctattcag tgtggcgcat ttcataacac cactggtgga ctgacctatt
    46261 tcaacacgac gccgctgggc cgcgccgtga ccggcaccat gctggttgct gctatgaaag
    46321 aagatggcgt gaatatctgg ggtgacggca gcacctataa aggaaacgat atcgaacgtt
    46381 tctaccgtta cggtctgctg accaatgctg aactgcagat ttacaaaccg tggcttgata
    46441 ctgactttat tgatgaactg ggtggccgtc atgagatgtc tgaatttatg attgcctgcg
    46501 gtttcgacta caaaatgtct gtcgaaaaag cttactccac ggactccaac atgcttggtg
    46561 caacgcatga agcgaaggat ctggaatacc tcaactccag cgtcaaaatc gtcaacccaa
    46621 ttatgggcgt gaagttttgg gatgagagcg tgaaaatccc ggcagaagaa gtcacagtac
    46681 gctttgagca aggtcatccg gtggcgctga acggtaaaac ctttagcgac gacgtagaaa
    46741 tgatgctgga agctaaccgc atcggcggtc gtcacggcct gggcatgagc gaccagattg
    46801 aaaaccgtat catcgaagca aaaagccgcg gcatttatga agcccctggg atggcactgc
    46861 tgcacattgc gtatgaacgt ttgttgaccg gtattcacaa cgaagacacc attgagcagt
    46921 atcacgcgca tggtcgtcag ttgggccgcc tgctgtacca gggacgttgg tttgattccc
    46981 aggcgctgat gctgcgtgac tctctgcaac gctgggttgc cagccagatc actggtgaag
    47041 ttactctgga gctgcgccgc gggaacgatt attcaatcct gaataccgtc tcagagaacc
    47101 tgacctacaa gccagagcgt ctgacgatgg aaaaaggcga ctcggtgttc tcgccagatg
    47161 atcgtatcgg tcaactgacc atgcgtaacc tggatatcac cgatacccgc gagaaacttt
    47221 tcggttatgc caaaactggc ctgctctcct cctctgccgc ttcaggggtg ccgcaggtgg
    47281 agaatctgga aaataaaggc cagtaattcg acatcaaccc tgcccttcag gggtggggtt
    47341 accttgatta tcctctgtaa actcctttct gagcagcaca taatcgaaaa catcatggtt
    47401 tgcatccaca acccgtgagt ctcctttata gtgtgcaatc acttcctcaa gcgtttgcgg
    47461 ctgttccggc tttgttaccc ccatccacgc attaataagg taattattgt aagctgtcga
    47521 aaagatgttg ttttccgttg tgcgatccac gccatcccca agaacggggc tataccagat
    47581 aaacatcggg acatgatacg cctgctggct agcctccctg cctccatgaa aatagacgtt
    47641 cttcttcgta gggtcgcgtt ccaggccgtg gtcggcaaaa tacatgaccg aggcgcggca
    47701 atcttttaat aattcaaaaa cctgacctag caaactatct gtgtaatgaa tggagttgtc
    47761 atagcaggca tcctgatcgt cctgcggttg aaacacagcg ctggattgcg gataggcgct
    47821 acaagccggt tcatggcttc catttaaatg aagaacaatc agtttctttt gctgcgtctt
    47881 ttgctgtaac gcttgcgata aatgcggcaa caacaattca tcaaatcctc tgacatagac
    47941 tgtttccatg gcgcgcatgg cgatactggt aactgctgta ccattctgcc gaaaagcgga
    48001 ttgcgagctt agccagaaag tctgaaatcc tgcttgatta gccatattaa taatgttgtc
    48061 cgggtaatta tgaatgtcat gactcaaaac agaatcagca gttaaagaaa ggggaaccga
    48121 cagcgcagtg taaggtgcac cgcttattgc ttgattaaac agtttgatct gctttctttg
    48181 tgcttcgact tgcggtgtcg tagagcgtgt atatccatac aaaaacatat tgtcgacacg
    48241 tacagactcc cccacaatca acacgtaggt atcaatacct gtatccctga ctgatagttg
    48301 aaaatacggc accgtgtttg caattgagag taatctttga tgctctttcg ctgctaaagc
    48361 aaaataattg agattgaaaa acggcgtata ggtagcaaat cgcgacgcta gtatatatgg
    48421 actgaacgcc tctttatttt ttgcatcttt ataagcaaat tgacaagcgg aaaataaact
    48481 gcccgaaatg acaatcagca ataatattcc tgtcactttt tttgtcggca aggaaacatc
    48541 atattttatt attaccgcca aaaaaagaag ggataaaaag gtaaaagcac atagataagg
    48601 gatatacatt cccagcattt tgacaacttc atccggatca ctctgcagca cactaatcgc
    48661 aaatccatca ttgaaagttg taccaaagat acaccatgaa taaaggctaa tactcatatc
    48721 tgccgcagtc agtaacacaa atggtacggc aataagcagg cgaagcggaa gggataatat
    48781 acgtttaaca accaggatgg ttagaacgaa gaacagcaat cgctgaacca tatgcgctcc
    48841 ggaagaagcg actaagttcg taataccaaa aacaataaca caaagataca acaaccaatg
    48901 agatttaaaa cttttaacaa atttgttgaa tactgtcatc cggaaatcca agttcacaac
    48961 caatataaac aattatcaac aatgttcaat tttatatcag acatgttttt caactctgat
    49021 ccatatcaga ccgaaacact gttagttttt tctttttaaa tgatgcggcc attgttctca
    49081 gtaattaaat aacattatca atgcgtctct aaacattacg cagaacgtag ccatcagtag
    49141 ttatactgac aggagcagga tggtttatcg agaagtaaac atatccgtat tcgtaccgga
    49201 actggagttg acacaataaa gtgccaatta tgtcagcaga agggaaaagt ttataactaa
    49261 ggcgtaccgg caccatcgtt tcaaagtacc agctacgagt aaagcaactg gacgagatac
    49321 agatacctga caaccattcc tcagaccagg accaaaacga aaaaagacgc ttttcagcgt
    49381 ctcttttctg gaatttggta ccgaggacgg gacttgaacc cgtaagccct attgggcact
    49441 accacctcaa ggtagcgtgt ctaccaattc caccacctcg gcacggatac tactttttag
    49501 ttcgggatat cgctggtcgg cttagccgga gcagctggct gagtttgttc ggttttcgcc
    49561 ggcgcactca ggttttccca ttcgctacct ttattggttt tattgctatt gatgttaccc
    49621 agcaccagac tgatgatgaa gaataacgtt gccagcagcg ccgtcatgcg ggtcatgaag
    49681 ttaccagaac cacttgaacc aaacagcgta gcggaagcgc ctgctccgaa ggaggctccc
    49741 atatcagcgc ctttaccttg ctgcagcatg atcagaccaa caaggccaat tgccacaata
    49801 aggaaaacta ctaaaagagc ttcgtacata atcaacctgt tccttgcgga gttgccgcgt
    49861 accaatgctt caaccaataa agcgggagtt tttaatcttt cccactgaag cgggtgtgaa
    49921 tactaaccaa agcgaatgac cttcgcaagg gcaattttat tgcattgtat caactgcgga
    49981 aaaaaacagc aaaaccgatg tgttcgctga gaaaaaaggc gacaatcgcc gcctttttag
    50041 ccagttatct aacgctttaa acggctttta ctgcatcggc gatgcggtgt gcaaattcag
    50101 tcacctgcgc ttcgtcttcg ccttccacca tcacgcgaat taacggttcg gtgccggatt
    50161 tacgcaacag cacacgtcca cggctaccca gcgccgcttc aacttctgcg gtcacagctt
    50221 tgactgattc atgctccagc ggatcgccgc tacctgcggt gtaacgcacg tttaccagga
    50281 tttgcgggaa cattttcatg ccgctgcaaa ggtcgtgcag gctcatatga ttacgcgcca
    50341 tcgccgccag cacctgcaaa ccagcaacga tgccgtcacc ggtagtggtt ttatccagca
    50401 ggatcacatg accggaattc tctgcaccga tacgccagcc tttctcctgc attttttcca
    50461 gtacgtagcg gtcacccact ttcgcgcgcg caaatggaat tcctaactgt ttcagcgcca
    50521 gttcaagccc catgttgctc atcaatgtac ccacagcgcc accacgcagc tggccctgac
    50581 gaagaccttc acgcgcgatg atatacatga tctgatcgcc atcgacttta ttgccttcat
    50641 ggtcaaccat aatcacgcga tcgccgtcgc cgtcgaaggc aataccgaga tccgcttttt
    50701 cagccagcac acgagcctgg agcgcgcgaa cgtcggtagc ccccacttcg gcattgatgt
    50761 ttacaccgtt cggctcacaa ccgatagcga taacgttcgc tcccagttca cgcagcacgt
    50821 tcggcgcgat gtgataagtc gcaccgtttg cacaatccac cacaatcttc agttcactga
    50881 ggctaagttc gtttgggaac gtggctttgc aaaactcgat atagcgaccc gcagcatcaa
    50941 cgatacggct ggctttaccc agttctgccg aatcaacaca gctgatctct ttttccattt
    51001 ccgcttcgat ggcctcttct actgcatccg gtagtttggt gccgtcgata gagaaaaatt
    51061 taatgccatt atcgtagaac gggttatgcg atgcagatat cacaattccg gcctctgcgc
    51121 ggaaggtacg cgtcagataa gccacggccg gtgttggcat cggaccagtg aagagtgcgg
    51181 aaaggcccgc agccgccaga cccgcttcca gtgctgactc cagcatgtag ccagaaatac
    51241 gcgtgtcttt accaatgatg atcttacggg agccgtggcg cgccagcact ttacccgcgg
    51301 cccaacccag cttaagcaca aaatcagggg tgatcggcgc atcccctaca cgaccacgaa
    51361 tcccatcggt accgaaatat ttacgattac tcatagcgtt tgttttcctt tgcagacaga
    51421 gtggcttcca ccacccgcat cgcttctacg gtttctttga cgtcatgaac acgaatgata
    51481 tgcgcgcctt gcatagcggc aatgaccgca caagccagac taccgctcag acgctcggac
    51541 ggccccacgt tcagcagctg cccaatcatc gattttcgtg acatacccac caacagcggc
    51601 aggttgaaat ggtgaaattc agccaggcgc gccagtaatg aatagttatg ggagagattt
    51661 ttaccgaaac cgaatccggg gtcgagcaac aatttctctt ttgcgatacc cgcctgctcg
    51721 caacgtgcta tttgctcaat aaaatagcga ttcacttctg caaagacatc gtcatacttc
    51781 ggggcttcct gcatggtctt tggatttccc tgcatatgca tcagacaaac cggtaaaccg
    51841 gtttctgcag ctgcctccag agcccctggt tcggaaaggg agcggatatc gttaataatg
    51901 tgagcgccaa ctttcgctga ctcacggatg acttctggtt tggatgtatc gaccgagatc
    51961 cagacttcga agcgttgagc aattgcctca accacaggaa taacacgttg caactcttct
    52021 tcaacgctaa cttccgccgc tcctgggcgc gtggactcgc caccaacgtc aatgatcgtc
    52081 gcgccagcgt tgatcatcag attcgcatgt ttcaccgcat ctatcagcga gttatgcgta
    52141 ccgccatccg aaaaggaatc aggcgtgacg ttgaggatcc ccattacgtg aggatggcta
    52201 aggtccagtg aagtgccctg ggcaaagagt ttcatggtgt tatccctggt atgaattgat
    52261 aagaaaaaac cccggagcac gccccggggt tttcggtaca aatacagtca tctgatgcgg
    52321 gaacttattt gtcgcctaac tgctctgaca tggtgttacc cgggttcggc gtacgcggtt
    52381 catcaaccgg acgaggagcc tttggactac cattgtcgcc agaattgtta gaagcgcctg
    52441 gttcttccca gcccgctggc ggacgtacat cgcgacgtgc catcaggtca tcaatctgcg
    52501 gtgcgtcgat agtctcatat ttcatgagag catctttcat cgcatgcaga atatccatat
    52561 tgtcggtcag aagctgacgc gcacgattat agttacgctc aatcagtgct ttcacttcct
    52621 ggtcgatgat acgtgcagtt tcatcggaca tatgtttcgc tttcgctacg ctacggccga
    52681 ggaacacttc accttcttct tccgcgtaca gcagtggacc caatttctca gagaagcccc
    52741 actgagtcac catgttacgt gccaggttgg tcgcaacttt aatatcgttg gacgcaccgg
    52801 tagaaacatg ttccggcccg tagatgatct cttccgccag acgaccaccg tacagcgtag
    52861 aaatctggct ttccagtttc tgacggctgg cgctgattgc gtcgccctca ggcaagaaga
    52921 aagtcacacc cagcgcacga ccgcgtggga taatcgtcac tttgtgcacc ggatcgtgtt
    52981 ccggcaccag gcgaccgata attgcgtgac ccgcttcgtg gtaagccgtc gattctttct
    53041 gcgcttccgt catcaccatg gagcgacgtt ccgcacccat catgattttg tctttcgctt
    53101 tctcgaactc aaccatcgac acaacgcgtt tgttgccacg agcagcgaac agtgccgctt
    53161 cgttcaccag gttcgccagg tcagcaccgg agaaaccagg agtaccacgg gcaatgattg
    53221 ccgcgtcgat atcgggtgcc aatggtacgc gacgcatgtg aaccttcagg atctgctcac
    53281 gaccgcgaac atctggcaag ccaaccacaa cctgacggtc gaaacggcca ggacgcagca
    53341 gcgctgggtc gagaacgtcc ggacggttag tcgcggcgat aacgatgata ccttcgttac
    53401 cttcgaagcc atccatctca accagcatct ggttcagagt ctgttcacgt tcatcgtgac
    53461 caccgcccag acctgcgcca cgctggcggc ctacggcgtc gatttcatcg ataaagatga
    53521 tgcacggtgc cgctttcttc gcctgttcga acatgtcacg aacacgggat gcacccacac
    53581 cgacgaacat ttctacgaag tcagaaccgg agatagtaaa gaacggaact ttcgcttcgc
    53641 ctgcaatcgc tttcgccagc agcgttttac cggtacccgg aggaccgacc atcaggacgc
    53701 ctttcgggat cttaccgccg agtttctgga agcggctcgg ctcgcgcaga tactcaacca
    53761 gttcagcaac ttcttctttt gcttcgtcgc agcccgcaac gtcagcaaag gtcgttttga
    53821 tctgatcttc agtcagcatg cgcgctttgc tcttaccaaa cgacatggca cctttgccac
    53881 cgccgccctg catttgacgc atgaagaaga tccagacacc aatcagcaac aacatcggga
    53941 accaggagat gaagatagaa gccagcaggc ttggttcttc aggcggttca ccgacaacct
    54001 tgacgttctt ggtcaacagg ttatccagta atttcggatc ctgaaccgga atgtaagtgg
    54061 tataacggtt actatctttc ttggtaacgt tgatttcacg tccgttgata cgcgcttcac
    54121 gaacctggtc gttattgacc tcttgtagga aggtagagta atccacctta cggccattag
    54181 actcgctggg cccaaagctc tggaatactg acatcagcac aacggcaatg accagccaga
    54241 gtattaggtt tttcgccatg tcactcaagg gattaacctc ttattacaac tgtgttaaaa
    54301 acagcgtcag gatactctat atccagcatc tttcaaactt tcgtctgaaa tctcccggtt
    54361 agggtttacg cccggtcgct acaatataca cttcccgcga acgtgcacga gaagagtccg
    54421 gcttacgaac tttgaccttc gtaaacaggg agcgaatttc ccttagatac tcatcgaaac
    54481 cttcgccctg gaacaccttc actacaaaac tgccacctgg cgctaataca tcacgacaca
    54541 tttctagcgc cagttccacc agatacatgg cacgggggat atccaccgcc ggtgttccgc
    54601 tcatgtttgg tgccatatcg gacatgacaa cctggacttt gctgtcgcca acgcgctcca
    54661 gcagtgcttt catcaccagt tcatcacgaa aatcgccctg aagaaagtcc acaccaacga
    54721 taggatccat aggtaaaaga tcgcaagcga tgatgcggcc tttgccgcca atttgggtga
    54781 ccacatattg tgaccaacca cccggagcag caccaaggtc gacaaccgtc attcccggtt
    54841 taaagagttt gtcactttgc tgtatttcat caagtttaaa ccaggcacgg gaacgtaacc
    54901 cctttttctg tgcctgttga acatatttat cgctaaagtg ttcctgaagc cagcggctgg
    54961 agctggcaga acgcttctta cctgtcattt aactttccca tggggataac tcatcgtaac
    55021 caattgcgta aatttttacg cgcctatttg gtgatatatg ggagatggcg gtagaatgac
    55081 ccgttttcaa tcccaacgta agcaaaaaat acgatgaatc tgagtactaa acaaaaacag
    55141 cacctgaaag gtctggcaca tccgctcaag ccagttgttc tgcttggcag taatggtttg
    55201 accgaagggg tgctggccga gattgaacaa gcgttagagc accatgaact catcaaggtg
    55261 aaaatcgcca ccgaagatcg cgaaactaaa accttgatcg tggaagctat cgtgcgcgaa
    55321 accggcgcct gtaatgtaca ggtcatcggt aaaacgctgg tgctttatcg cccaactaaa
    55381 gaacgtaaaa tctcgctgcc acgctaagat tatcctaaag ttacacacat tcgctgtgta
    55441 aaacgagggg ttttccgcag gcaggagagc aaaatgccac gctctgttcg ttgataaaag
    55501 gccgcatagc ggcctttttc ctttctttac aatacatcaa catcttgagt attgggtaat
    55561 tcttacaggt attccacctt aattacttca aattctactt cgccgcccgg cgttttgatg
    55621 accacaacat catcttcttc tttgccgatc aggccacgag caataggcga gtttacagaa
    55681 atcaggtttt gtttaaagtc agcttcgtca tcgccaacga tgcgataagt ctgttcttcg
    55741 tcagaatcga gattcagcac cgttacggta gcaccaaaaa taacgcgccc attgttgggc
    55801 attttggtga catcaatcac ctgcgcgttc gacagcttgg cttcgatgtc tttgatacgg
    55861 ccttcgcaaa agccctgctg ttcacgagct gcgtggtatt cggcgttttc tttcaggtcg
    55921 ccatgctcac gcgcttccgc gatagcagca atgatttcag gacggcgcac agatttcaga
    55981 aaatccagct cttcgcgtaa tttttcagcg ccgcgtaagg tcatcggaat agcttgcatt
    56041 tgttatacct cttgaatatt cctgatgggg caagtcttca cccaccccgg ctgttcggcc
    56101 tgcccggcat aactcttcac cgggaccaga agcaaaaaat accgacccgg gtacaagtcc
    56161 caggtcagct acaattcaca ttttgatagt cattttaccc tgaagttccc gaagggtcat
    56221 cgtttacttt atagggcgtt gcgccgtagt atgacggctc gattccaggt tgttagcgcg
    56281 agattatgcg attttccaga tttatcatcg gattgaccag ctgtatagcg ttcagtgttc
    56341 aggccgcaaa tgttgatgag tacattactc aactccccgc tggtgccaac cttgccctga
    56401 tggtgcaaaa agtcggcgcg tcggcccccg ctattgatta ccacagtcag cagatggcgc
    56461 tgcccgccag tacgcagaaa gtgattactg cactggcggc gttgattcaa ctcggccccg
    56521 attttcgttt taccacgacg cttgaaacca aaggcaatgt ggaaaacggc gtacttaagg
    56581 gtgacttagt ggcgcgattt ggtgccgatc cgacgttaag acgtcaggat attcgcaata
    56641 tggtcgcgac tttgaaaaaa tctggcgtca accaaatcga tggcaatgtg ttgatagata
    56701 cctccatttt cgccagccac gataaagccc ccggctggcc atggaatgac atgacacaat
    56761 gctttagcgc tccgcctgcc gccgccatag ttgaccgcaa ctgtttctcc gtctcgctct
    56821 acagtgcccc aaaacctggt gatatggctt ttatacgcgt ggcatcttat taccccgtta
    56881 cgatgttcag ccaggtacgc accctccccc gtggttctgc cgaagcgcaa tactgcgagc
    56941 tggatgtggt gccaggcgac ctgaaccgct ttacgctgac cggatgcctg ccacaacgtt
    57001 ctgagccgct cccgttggct tttgccgtgc aggatggagc cagctatgcc ggtgcaattc
    57061 tgaaagatga gttaaaacag gcaggtatca cctggagcgg aacactgctg cgccagactc
    57121 aggttaacga acctggaacg gtagttgcca gtaaacagtc ggccccgctg cacgatctgc
    57181 ttaagattat gctgaaaaag tcggacaaca tgatcgccga tacggttttc cgcatgatag
    57241 gccatgcgcg cttcaatgtg cctggaacat ggcgggccgg gtcggacgcc gtgcgtcaga
    57301 tcctgcgcca gcaagccggt gtcgatattg gaaacaccat tattgccgat ggttcagggc
    57361 tttcgcggca taacctgatt gcccccgcca ccatgatgca ggtgctgcaa tacattgccc
    57421 aacacgacaa tgaacttaac tttatctcca tgctgccgct ggcgggctat gacggctctt
    57481 tgcagtaccg tgcaggtctg catcaggcgg gcgtggatgg aaaagtctca gcgaaaaccg
    57541 gttcgttgca gggggtatat aacctggcgg gattcattac cacagcgagc gggcaacgaa
    57601 tggcgtttgt gcaatatctt tctggctatg cagtagaacc ggcggatcag cgtaatcgcc
    57661 gtattccgtt ggtacgtttt gaaagccgtt tgtataaaga tatttaccag aacaattagt
    57721 ctaaagagaa accccggcac atggccgggg ttttgctgat taacgcttgt aaatgaactc
    57781 aacgccttct tcgtcgtctt cgtcccagtc gtcatcccag tcttcatcat cctcttcagc
    57841 aatctcttca agctgctggc gatgataatc atcccacatg aattcgactt tctctggctg
    57901 tttcgcttct tcagcctgca cgaccgggtt ttcaatgata aaggtcatca catcccagca
    57961 gagatctttc acgcccagtc cgctcgccgc agagatcaga taatatttat cttcccaacc
    58021 cagcgcctca gcgatcgctt tcgctttctc ttcggcttct gccttatcca gcaggtcgat
    58081 cttgttgaag actaaccaac gcggtttcgc cgccagatcc tggctgtatt tttccagctc
    58141 gctgataata atacgcgcgt tttcaaccgg atcggtgccg tcaatcggat cgatatcgat
    58201 gaggtgcaac agaacgcggc aacgttccag gtgcttcagg aagcgaatgc ccagacctgc
    58261 gccttctgca gcgccttcaa tcagtcctgg aatatcggca acaacgaagc tcttttcgtt
    58321 gtccattcgt accacaccaa gacttggcac cagagtggta aacggataat ccgccacttt
    58381 cggtttagcc gccgataccg cacgaataaa ggtcgattta cccgcgtttg gcatccccaa
    58441 cataccgacg tcagccagca gcatcagctc cagcagcagc tcacgcttat cgcccggcgt
    58501 accgttagtt ttctgccgtg gtgtacggtt aactgacgat ttgaaacggg tattgcccag
    58561 accgtgccag ccgcccttag caaccagcag acgctgaccg tgtttggtca tatcacccat
    58621 ggtttcacca gtaccctggt cgattacacg cgtacctacc ggcactttaa tcgtgacgtc
    58681 tttaccgcgc ttaccggtac agtcgcggct tgcaccgttt tgaccgcgct ctgcacggaa
    58741 agatttttca aaacgataat cgataagcgt gttcaggttc tcgtcggctt ccatccatac
    58801 gtcaccacca tcaccgccgt cgccgccatc cgggccgcct ttcggaatat acttttcgcg
    58861 gcggaagctc acgcaaccat taccgccatc acctgcaacg accagaatcg atgcttcatc
    58921 aacaaacttc attttattct ccgtaaatca ttcgcctgcg cggggttgcg ataccaccgt
    58981 tgtatgctta cgtaatccgc cccaaattcg atgaccaatg gcggaataca tcgcgcccgc
    59041 aaccacgaca aacgcaccga gataacctaa aaggtttaac atcggtctgg cgaagaaatc
    59101 gggccaggcc agtgataaaa gatctgaaaa aaacagcgta aacagtgggg tgagcgtgat
    59161 gatcgcgctc acctgcgctg cctgccagcg agccatcgct tccgccaggg cgccatatcc
    59221 taccaatgta ttcagtccgc aaaaaattaa acatgcgagc tgccagtggc taagctgcgc
    59281 tatcactcca ggatttgcca gagggaagag cgcaattgta cataaagtgt acagcaaaaa
    59341 caggatctgc ggtgaggcca gccgacgcaa taaaaccttt tgcgccacgc cataactcac
    59401 ccaaaccgtc gccgcaccga ccccaaagat aactccccag gtgtaatcgg tgagctttgt
    59461 aaatatctcg accagactgg tgttaaaaaa catcaccagg ccgctcagga gcatcaatgc
    59521 ccctacaacc tgagtactgc gcattttctc tttcaggata aatacgctgg caaccatcat
    59581 gccaactggt gagagttgcc caatcacctg cgaagcggtc ggactcaggt attgcaagga
    59641 tgagctgaac aggatgaagt tcccaaacag cccggcggtc gccactgcca acaaaatcaa
    59701 ccagcgtggc ttacgaaaca cgcgtaatgg cggcaaccgc ttcttcaccg caagaatggc
    59761 accaaggcca atactcgcca tcaagaaacg gtaaaacacg attgtcggag gttccatcac
    59821 ctccagcacc tgcttcattg cgattggcaa cgccccccag caaattgctg tggtgagcgc
    59881 caaaagaatg ccaatgcctg cctgctgctt cataccgtat tccctgcaaa gcgcatttgc
    59941 ggattaacgt cgcaccatgc gcatttttac cggttatcga atgtaaaaag ccccgcaacg
    60001 tgttgcgggg ctttcatccg ttaccgggac gcgaaaaact tattcagctt cgatgctgat
    60061 aaatttacgg tttttcgggc ctttaacttc gaatttcact ttaccgtctg ctttagcaaa
    60121 cagagtgtgg tcacgaccgc aacctacgtt agcgccagcg tggaatttgg taccacgttg
    60181 acgaacgatg atgctacccg ccagaacgga ttcgccaccg aaacgcttaa cgcccaggcg
    60241 tttagcttct gaatcgcgac cgttacgtgt ggagccgcca gcctttttat gtgccatttg
    60301 aaatctctcc tcaggtctta ggcgctgatg ccagtaattt tcacatcagt gaaccactga
    60361 cgatggccct gctgcttacg atagtgttta cgacgacgaa acttaacgat tttaactttc
    60421 tcgccacgac cgtgagcaac aacttcagct ttgattacgc cgccatcaac gaaaggaacg
    60481 ccgattttga cttcttcacc gtttgcgatc atcagcactt cagcgaactc aacagtttcg
    60541 ccagttgcga tgtccagctt ttccaggcga acggtctgac cttcgcttac tcggtgttgt
    60601 ttaccaccac tttggaaaac cgcgtacata taaaactccg cttccgcgca caccttttca
    60661 atgattcaga gtgcgctata aatattcaca atagggcgcg aatattacgc aaaacgcacg
    60721 cctttgacaa gtgctacagt caatacacga agaaaaaaaa cacaacttgt acggtaacgt
    60781 ttatctgtgc cattttttca gtacaatcac cctatattcc taaccataaa ccctaagttg
    60841 cctttgttca cagtaaggta atcggggcga aaagcccggc ttttgcgatg aatttagaaa
    60901 aaatcaatga gttaaccgcg caagatatgg cgggtgttaa tgcggcaatc cttgagcagc
    60961 ttaattccga cgtccaactg atcaatcagt taggctatta catcgtcagc ggcggcggta
    61021 aacgtattcg tccgatgatt gctgtactgg ctgcacgagc tgttggctat gagggaaatg
    61081 cgcatgtcac cattgctgcc ctgatcgagt ttatccacac ggcgactctg ctgcacgacg
    61141 acgttgtgga tgaatcagat atgcgcaggg gtaaagctac cgccaacgcc gcatttggca
    61201 atgccgccag cgtgctggta ggcgatttta tttatacccg cgcgttccag atgatgacca
    61261 gcctcggctc gctcaaagtg ctggaagtca tgtcagaagc cgtaaacgtc atcgcagaag
    61321 gtgaagttct gcaactgatg aacgttaacg atccggacat cactgaagaa aactacatgc
    61381 gcgttatcta tagcaaaacc gcgcgtctgt ttgaggctgc cgcgcagtgt tccgggattc
    61441 tggctggctg tacgccggag gaggagaaag gcctgcagga ttatgggcgc tatctcggca
    61501 ctgctttcca gttgatcgac gatttactcg attacaatgc cgatggcgaa cagttaggta
    61561 aaaacgtcgg cgacgatctg aacgaaggta aaccgacgct gccgctgctg catgcaatgc
    61621 atcatggcac accagaacag gcacagatga ttcgtaccgc catcgaacag ggtaacggtc
    61681 gccatcttct ggaaccggtt ctggaagcaa tgaacgcttg tggatctctt gaatggacgc
    61741 gtcagcgtgc cgaggaagaa gcagacaaag ccatcgcagc gttacaggtg ctcccggaca
    61801 ccccttggcg agaagcactc atcggcctcg cgcacatcgc tgttcaacgc gatcgttaat
    61861 cccctcccct catcccgcgc agcgcgcggg atgattccag taaattccat aaaaacactt
    61921 attcagctct ataacacctg aaaaataggg ctgtacatct ttatatattc tgaatattca
    61981 cactctttac aggaactttt tagagcaatg ggccatcagg agtatagtga tgctcgacag
    62041 aagaagtgtt ctgaatgaaa gcgactaact taaggagtga ggaaagatgg aaagtaattt
    62101 cattgactgg catcccgctg acatcattgc aggtttgcgc aagaagggaa cgtcaatggc
    62161 ggcggagtct cgcagaaatg gtttgagttc ctcaacgctg gcgaacgcat tatcacgccc
    62221 atggccgaaa ggagagatga ttattgcgaa agccctggga actgacccct gggttatctg
    62281 gccatcacgc taccatgatc cgcagaccca tgagtttatc gacagaacgc agttgatgcg
    62341 tagctacact aaaccgaaaa aatgagtggt ctggcggtag ccccgcgaac ggggctgcca
    62401 gctctcagac aattactcgc ctttcacacg ctcaatattt gcacctaaag cgcgtagttt
    62461 gtcttcaatg cgttcgtagc cacgatcgat gtgataaata cgatcaacca ccgtcgtccc
    62521 ttccgcaata cagccagcca gcaccaggct tgctgatgca cgcagatcgg ttgccataac
    62581 ctgtgcgcca gaaagttttt caacaccgtg acaaataacg gtattgcttt cgatttcggc
    62641 gtgcgcgccc atacggctca gctctggcac atgcataaag cggttttcaa agaccgtttc
    62701 ggtgatgaat ccggtccctt ctgccaccag gttcaacagc gtgaactggg cctgcatatc
    62761 ggtcgggaat gccggatgcg gcgcggtacg tacgttaaca gccttcggac gtttgccatg
    62821 catatccagg ctaatccagt cttcgccgac ttcgatgtcc gctccagcgt cacgcagttt
    62881 cgccagcacg gcgtccagag tatctggctg cgcgttacgg cagataattt tgccgcgaga
    62941 gatcgccgcc gccaccagga aagtaccggt ttcgatacga tccggcagca cacgatagac
    63001 accgccgcct aaacgttcca caccttcgat gacgatacga tcggtgccct gaccgctaat
    63061 tttcgcaccc agcgtaatca ggaagttcgc ggtatcgacg atttccggtt cacgcgctgc
    63121 gttttcaata atcgtggtgc cttccgcaag ggttgcagca cacatgatgg tcaccgttgc
    63181 gccaacgctg actttatcca tcacgatatg tgcgcctttc aaacgaccat cgacggaagc
    63241 tttaacgtaa ccttcttcca gtttgatggt cgcgcctaat tgttcgaggc cagaaatgtg
    63301 tagatcaacc ggacgtgcgc cgatcgtaca accgccaggc agtgaaactt gcccctgacc
    63361 aaagcgcgct accagcggcc ccagcgccca gatagaagca cgcatggttt taaccagatc
    63421 gtaaggtgcg cagaatacat taacgtcgcg ggcatcaata tgcacagaac cattacgttc
    63481 tactttcgca cccagctggc ttagcagctt cattgatgta tcgacgtctt ttagtttcgg
    63541 gacgttctgg atctctaccg gttcttccgc cagtagtgcg gcaaaaagga taggcagagc
    63601 agcattttta gcgccggaaa ttgtgacttc gccctggagc ttcgttggcc cctgaacacg
    63661 aaatttatcc atttagtttg ttctcagtta acaattcata tccgctaccg gcgaatcgcc
    63721 catagctcaa aagccgttca gtttgcgatc gcgcgcccac tccgcagggg tatacgcttt
    63781 gatcgacaca gcatgaatgc ggttatccgc aatatattcc atcagcgggc catagaccgt
    63841 ctgctgtttt ttaacccgac tcatgccgtc aaacaactca cccacggcaa taacctgaaa
    63901 gtggctgcca tcgccggaaa cgtggacttc ctggagggag agagcgttca tcaacacgct
    63961 ctgaatttca ttattttcca tgggatcttc aatcatcagt ttataaatca gcgaaacatc
    64021 ttagagcaaa gttgcgctgg cataaataag caaaaagcct cgctgataaa tcagacaagg
    64081 ctcgacttgc aggcaggttt gccggacagg cggttaacgc catatccggc ctgaaaaaat
    64141 ttaacgaggc agaacatcag caggcaaatt atacaatttc gccagggtat acactttgtc
    64201 gtttaccccc tgaagcgtca cattgttgcc ctgctttttc gccagatcga taagatggag
    64261 cagcagtgcc agtccccccg tatccacgcg agacacacgg ctaagatcga tgctggtaat
    64321 ccccttcacc gcttcctcac gtatttccca aagcggtagc agaacgtcct gatccagctc
    64381 tccggataac gccagcgtgt cacccgtctg catccagctc agtgactcgc tcattatttt
    64441 ttctcttcca gagtgatttt ctgttgagaa atcgatttca gttgcgcagt caggccgtcg
    64501 atacctttgg tacgcagcag cgttccccac tcgttttgtt tggtggtgat catactgacg
    64561 ccttcagcaa tcatgtcgta agcctgccag ttgcccgtct gggagttttt acgccactgg
    64621 aagtccagac gcaccggcgg gcggccattc gggtcaataa tggtaacgcg aatcggcaca
    64681 atggttttat cacccagcgg ctgttctggc gcaatctgat aggtttgacc gtgatacatc
    64741 gccagcgcct gaccgtaagc ctgcttcagg tactcacgga aagcggcaaa gtaagcatca
    64801 cgttgagctg gggtcgcact cttgtaatac tggcccagca ccagcgcacc ggcgtatttc
    64861 acctgtacgt atggcaacag ttcctgatca acaatggtac gcagataatc cgggttagcc
    64921 cgaatttgcg gttgctcatt cttcaggcga tcgaacgttt tctgcgccgc ctcgtccatc
    64981 agcttatacg gattggtctg gtctgccgcg gttgccgcac tcagcggtgc aatcaccagc
    65041 aaagcgacca tcattaaacg tttaaacatg cgtcggttct cctgaaatta tttcgttgta
    65101 cccacaggtt cagtggtttc attattacct ggcgcagcag ctggcgcatc gccactattc
    65161 ttattgtcat cgcctttact accgtaaagg aactgaccaa tgagatcttc cagcaccatc
    65221 gcagacttgg tgtcctgaat tgtatcgcca tccttcagga tagcagttcc cagttccggg
    65281 tcttcaaaac cgacgtttaa tgccagatat tgttccccca gcaggccgga agtacgaatg
    65341 ctcagcgaac tggtatcagg aatgtggtta taacgttgtt caatttccag cgttacgcgc
    65401 ggcagatagg ttttcgggtc cagcgtaata tccgccaccc ggcccacaac aacgccacca
    65461 atgctgaccg gagagcgggc tttcaggccg ccaatgttat cgaacgtcgc ataaagcgta
    65521 taggtcggtt cagtacgtat agacgtcacg ttcgctgcct tcaggcaaac aaacagcgcc
    65581 gcaagcagtg ctgctaataa aaagataccc acccaaattt catttttttt cgtttgcatg
    65641 aactcaattc ccaaacatca atgcggtcag cacaaaatcc agccccagaa cagccagaga
    65701 cgagtggaca acggtgcgag tggttgcccg gctaatcccg gcagacgtcg ggatggcgtc
    65761 gtagccgtta aacaacgaaa tccacgtcac cgtgatggcg aacaccacgc tcttaatcag
    65821 acagttgacg agatccatac gccagtcgac ggcattttgc attgccgacc agaagaaccc
    65881 actatcaatg cctttccaac tgacgccgac cagcgatccg ccccagatcc ccacggcgac
    65941 gaagataacc gtcaacagtg gtaatgaaat aactccagcc cagaaacggg gagaaataac
    66001 ccgacgcagc ggatccaccg ccatcatctc catactggag agttgctctg tagcgcgcat
    66061 caggccgatt tctgcggtta gcgccgaacc agcacgcccg gcaaacaaca acgcggcaac
    66121 caccggcccc agttcacgca gtagcgataa cgccaccagc atacccagac tggtttccgc
    66181 actataagtg gtcagaacca gataaccttg cagccccaac accattccga tgaacacgcc
    66241 agaaacaaca ataatcagca tcgacaggac gccgacatta tagagctggc gcaccagcag
    66301 cggtgcatgt ttgcgaaatt ccggtttgcc gaccagcgca ttgaataaca ttaacccggc
    66361 ccgcccgaac gttctcaggg ttttaatccc tttatgtccg agcgacgcca gcgcatttaa
    66421 cagcatgagt ggcttaactc cctggtaaaa gatcagcgtg ataatcgccg gcaggatagc
    66481 ggaacggaac aggcccgtca gctatcccgt ccagaaactg acgtacgcgc ggatcaggat
    66541 tcgcctgcaa cgcctgggca ctgccatgag cgacaatttt tttgtccgcc aggatccagg
    66601 cgtgatccgc aatacttaac acttccggca catcgtgaga aaccaccaca caagtcacgc
    66661 ccagcgcgct gttcagctca gaaatcagct tcaccagcac gcccatggta atgggatctt
    66721 gcccaacaaa gggctcatca aacatgatga gatccggctc cagcgcaatc gcacgtgcca
    66781 gcgctgcacg ccgcgccatc ccaccggaaa gttcagaagg cataagttta gccgctccac
    66841 gcagccccac ggcctccagc ttcatcatca ccgtactatg caacaatggc gcgggaagtt
    66901 gggtatgttc ccgcagtgga taggcgacgt tgtcaaatac gttcatatca gtgaacaacg
    66961 ccccggactg aaataacatg ctcatccgtt tgcgcactgt atacaggcgc gaacgagaca
    67021 tcgccggaat attctcacca tcgaaaagga tctcaccatg atctggtgcg atttgcccgc
    67081 caatcagacg gagtagcgtc gttttaccga tgcccgatgg ccccatgatc gccgtgatct
    67141 tccctcgcgg cacggtcagg gaaatattat cgaagatgca gcgattgcca cgcgtaaaac
    67201 tgacatcgcg catatcgact aaattcgcca cagactgctc cataattcac ccttcgtctt
    67261 gcgttgattt tctaagcatg gcgctcaatt taaccttgaa cccaacatat ttacagaata
    67321 ttacctgccg tggttagcga aagctggcat ttgttttact ttttagccgc ataaagtcaa
    67381 aattaagaat ctgttacggc tttctgaaaa tcttcagcgg accggcgagt atacctgaag
    67441 aaaggacgtt agatgctttt agctacggca ctgttaattg ttggtttact tttggtcgtt
    67501 tacagtgccg accgcctggt ttttgccgcg tctattcttt gccgaacctt tggcatcccg
    67561 ccgttgatca tcggcatgac ggtggtcagt attggtacat cgttaccaga aatcatcgtc
    67621 tcgcttgccg cgtctttgca cgaacaacgc gatttagccg ttggtacagc cctcggctca
    67681 aacattatca atatattgct gatcctcggt ctggctgcgc tggttcgtcc ttttaccgtc
    67741 cattctgatg ttctacgccg tgaattaccc ttaatgttgt tggtcagcgt ggtggccggt
    67801 tccgtactct atgacggaca acttagtcgc agcgatggta tctttctcct ctttctggct
    67861 gtgctatggc tgctgttcat tgttaaactt gcacgtcagg ctgaacgtca ggggactgac
    67921 agcctgacca gagagcagct tgcagagctg ccgcgtgaag gtggattgcc cgtcgcattt
    67981 ttatggctcg gcatcgcact gatcattatg ccagtggcca cgcggatggt agttgataac
    68041 gccacggtgc tggcgaatta ctttgccatc agcgagttga cgatgggtct gacggcaatt
    68101 gctatcggaa ccagcctgcc ggaactggca accgcaatag cgggggttcg caaaggtgaa
    68161 aacgacattg cggtcggaaa tatcattggc gcaaacattt ttattattgt catcgtgttg
    68221 ggtttacccg cgctgataac gccaggagag attgatccac tggcgtacag tcgtgactac
    68281 agcgtgatgt tgctggtgag cattattttt gcgttgctgt gctggcggcg ctccccgcaa
    68341 ccgggccgtg gtgtaggggt attattaact ggcggattta tcgtatggct ggcgatgttg
    68401 tactggttat cgccaatact cgttgaataa ctggaaacgc attatgtcgc acgtagagtt
    68461 acaaccgggt tttgactttc agcaagcagg taaagaagtc ctggcgattg aacgtgaatg
    68521 cctggcggag cttgatcaat acatcaatca gaatttcacg cttgcctgtg aaaagatgtt
    68581 ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa tcggggcaca tcgggcgcaa
    68641 aatggcagca acatttgcca gcaccggtac accttcattt ttcgtccatc ctggcgaagc
    68701 cgcgcatggt gatttaggca tggtcacccc acaggatgtg gtgattgcta tctctaactc
    68761 aggtgaatcc agcgaaatca cggccttgat tccagtgctt aagcgtcttc acgtaccgtt
    68821 aatctgcatc accggtcgcc cggagagcag catggcgcgc gccgcagatg tgcatctgtg
    68881 tgttaaagtg gcgaaagaag cctgtccgtt agggctggca ccgaccagca gcacaaccgc
    68941 cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg gctttactgc
    69001 tgaagatttt gcgctctcac acccaggcgg cgcactgggt cgtaaacttc tgctgcgcgt
    69061 aaacgatatt atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg ccagtctgcg
    69121 tgacgcattg ctggaagtta cccgcaaaaa tcttggtatg actgtcattt gcgatgacaa
    69181 tatgatgatt gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg atatgggcgt
    69241 ggatgttcgt cagttaagta ttgccgatgt gatgacgccg gggggaatac gcgtgcgccc
    69301 aggcattctg gccgttgagg cactgaactt aatgcagtcc cgccatatca cctccgtgat
    69361 ggttgccgat ggcgaccatt tactcggtgt gttacatatg catgatttac tgcgtgcagg
    69421 cgtagtgtaa agattcaagg ataaacaaca atgagcaaag caggtgcgtc gcttgcgacc
    69481 tgttacggcc ctgtcagcgc cgacgttatg gcaaaagcag agaacattcg tctgctgatc
    69541 ctcgacgttg atggcgtact gtcagatggc ctgatttata tgggcaataa cggcgaagag
    69601 ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac ctctgatatt
    69661 gaagtcgcta tcattaccgg gcgaaaggct aaactggtag aagatcgttg tgccacattg
    69721 gggatcactc acttgtatca ggggcagtca aacaaactga tcgcctttag cgatctgctg
    69781 gaaaaactgg cgattgcccc ggaaaatgtg gcttatgtcg gcgatgatct catcgactgg
    69841 ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc actgttgatc
    69901 ccgcgcgccg attacgtaac gcacattgct ggcggtcgtg gcgcagtgcg agaagtttgc
    69961 gacttattac tcctggcgca gggcaaactg gatgaagcca aagggcaatc gatatgagta
    70021 aagccagacg ttgggttatc attgtgctat cactggcggt tctggtgatg atcggcatta
    70081 atatggccga aaaagacgat accgcccagg tggtcgtcaa caacaatgat cccacctata
    70141 aaagcgagca tacggacacg ctcgtctata acccagaagg ggcactaagc tatcgattga
    70201 ttgctcaaca cgttgaatat tattccgatc aggccgtttc gtggtttacg cagccggtac
    70261 ttaccacgtt tgataaggat aaaatcccga catggtccgt aaaagcagat aaagccaagc
    70321 tgaccaatga ccggatgctc tatttatatg gacacgttga agtcaatgca ctcgtgccgg
    70381 actctcaact tcgcagaatc acgacggata acgcgcagat caatctggtg acgcaggatg
    70441 ttacctctga agacctcgtc acgttatacg gaacaacatt taactccagc ggtctgaaaa
    70501 tgcgcggcaa cttacgcagc aagaacgccg agctgattga aaaggttaga acatcctatg
    70561 aaattcaaaa caaacaaact cagccttaat cttgtgcttg ccagctcact tctggccgcc
    70621 agcattccgg catttgccgt aaccggagac actgatcagc cgatccacat tgaatcggac
    70681 cagcaatctc ttgatatgca aggcaacgtg gttaccttta ccggcaatgt catcgtcacc
    70741 cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg cggcgaacaa
    70801 ggtaaagaag tgattgacgg ctacggtaaa ccggcaacgt tctaccagat gcaggacaac
    70861 ggtaaacccg ttgaaggtca cgcttcacag atgcactacg aactggcaaa agattttgtc
    70921 gttctgacgg gtaatgctta tctgcagcag gtcgacagca acattaaggg cgataagatc
    70981 acttacctgg tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa gcgcgtaacc
    71041 accgttctgg tgccgtcgca gctgcaggac aaaaacaata aaggccagac cccggcacag
    71101 aagaagggta attaattcgt tatggcaaca ttaactgcaa agaaccttgc aaaagcctat
    71161 aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca actccgggga aattgtcggt
    71221 ctgctggggc caaacggtgc cggtaagacc accactttct acatggttgt tggcattgtg
    71281 ccgcgcgatg cgggcaacat cattattgat gatgacgata tcagtctgct gcctctgcat
    71341 gcacgcgcgc gccgcggtat cggctatctg ccgcaggaag cctccatttt ccgtcgcctc
    71401 agcgtttacg ataacctgat ggcggtactg caaattcgtg acgacttgtc tgctgaacaa
    71461 cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca ttgagcacct gcgtgacagc
    71521 atggggcagt cactctccgg gggtgaacgt cgccgtgtag aaattgcccg cgcactggct
    71581 gcaaatccga aatttattct gctcgacgaa ccgtttgccg gggttgaccc gatctcggtt
    71641 atcgacatta aacgcatcat tgagcacctg cgcgacagcg gcctgggcgt actgatcact
    71701 gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt cagtcagggg
    71761 catttgatcg cccacggcac gcctacagaa atcttacaag acgaatacgt taagcgtgta
    71821 taccttgggg aagacttcag actctgatag ggtagaagtt tgcgacgttt tagcaggaga
    71881 gtacgattct gaacatgaag caaggtttgc aactcaggct tagccaacaa ctggcgatga
    71941 cgccacagct ccaacaggca attcgtctgt tgcagttgtc gacgctggaa cttcagcagg
    72001 agctacagca ggcgctggag agcaatccgc tgcttgagca aatcgacact catgaagaaa
    72061 tcgacacccg cgaaacgcaa gacagtgaaa cactggacac cgccgacgcg ctcgaacaaa
    72121 aagagatgcc ggaagagctg ccgctcgatg ccagttggga caccatttac accgctggta
    72181 caccatccgg caccagcggt gactacattg acgacgagct gccggtctat cagggcgaaa
    72241 cgacacagac cttacaggat tacctgatgt ggcaggtcga gttgacaccg ttttccgaca
    72301 ctgaccgcgc tattgctacc tctatcgtcg atgccgttga tgacaccggt tatctgactg
    72361 tcccgctgga agatattctc gaaagtatgg gcgatgaaga gattgacatc gacgaagttg
    72421 aagccgtcct taagcggatc caacggtttg atccggtcgg tgtggcggca aaagatctgc
    72481 gtgactgcct gctgatccaa ctctcccaat tcgataagac cacgccatgg ctggaagagg
    72541 caagactgat cattagcgat catctcgatc tattagccaa tcatgacttc cgcactttaa
    72601 tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg atccagtcgc
    72661 tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc attccagatg
    72721 tgctggtgcg taagcataac ggtcactgga cggtagaact caacagtgac agcattccgc
    72781 gtctgcaaat caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac gatggtgata
    72841 gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt ctggaaagcc
    72901 gtaacgatac gctactgcgc gtgagtcgct gtatcgttga acagcagcaa gccttctttg
    72961 agcaaggcga agaatatatg aaaccgatgg tactggccga tatcgcccag gccgtcgaga
    73021 tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata cctgcatagt ccacgaggca
    73081 tttttgaact gaagtatttc ttttccagtc acgtcaatac cgagggcggc ggcgaagctt
    73141 cctccacggc gattcgtgcg ctggtgaaga aattaatcgc ggcggaaaac ccagcgaaac
    73201 cgttgagcga cagcaagtta acctctttgc tgtcggaaca aggtatcatg gtggctcgcc
    73261 gcactgttgc gaagtaccga gagtctttat ccattccgcc gtcaaaccag cgtaaacagc
    73321 tcgtttgacc caaccgataa ggaagacact atgcagctca acattaccgg aaataacgtc
    73381 gagatcaccg aggcactgcg cgaatttgtt acagccaaat ttgccaaact tgagcaatat
    73441 tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac ccacacctca
    73501 gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg tcaggacatg
    73561 tacgctgcca ttgatggctt aattgataag ctggcacgcc agttgaccaa acataaagat
    73621 aaactgaaac aacactaatt gtccgggcaa ttagcatgtg catggcggcc tgttgtgcgg
    73681 cacaacgggc catttgtacg gttaatgctc cgagcctgtt ccactgtttg agtgggcagg
    73741 ttcttaggtg aaattatgac aaataatgat acaactctac agcttagcag tgttcttaac
    73801 agggaatgta cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct ggaaatcatc
    73861 agcgagctgg cggcgaaaca acttagcctg gcgcctcagg tggtttttga agctatcctg
    73921 acgcgggaaa aaatgggcag taccggtatc ggcaatggta ttgccattcc gcatggcaaa
    73981 ctggaagaag atactctgcg tgccgttggc gttttcgttc agctcgaaac gcctatagct
    74041 ttcgatgcca tcgacaacca accggtagac ttactttttg ccctgctggt gccggcagac
    74101 caaactaaaa cgcacctgca tactctgtcg ctggtagcga aacgtctggc ggacaaaacc
    74161 atctgccgcc gtttacgcgc agcccagagc gatgaagagc tgtatcaaat cattacggat
    74221 accgaaggta ctccggatga agcgtagtta ttcggtaatg tctcttttag acgttgtgag
    74281 gagaaacagt acatggtact gatgatcgtc agcggacgtt caggttcagg taaatctgtc
    74341 gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg ataaccttcc cgtagtgttg
    74401 ttacccgatc tggctcgaac tctggccgat cgagagattt ctgccgccgt cagcattgat
    74461 gttcgtaata tgccggagtc accagaaata ttcgaacagg cgatgagtaa cctgcctgac
    74521 gctttctcac cgcaactact gttcctggat gccgaccgta ataccttaat tcgtcgttac
    74581 agtgacacgc gccgactgca tccgctttcc agcaaaaacc tgtcgctgga aagtgctatc
    74641 gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag cggatctgat tgtcgatacc
    74701 tcagaaatgt ccgttcacga gctggcagaa atgctgcgta cccgtctgct gggtaaacgt
    74761 gaacgtgaac tgaccatggt ctttgagtct ttcggcttca aacacggtat ccctatcgat
    74821 gcagattacg tctttgacgt gcgcttcttg ccgaacccgc actgggatcc gaaactgcgc
    74881 ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg accgccacac agaagtacac
    74941 aattttatct accagacgcg aagctatctt gagctatggt tacctatgct ggaaaccaac
    75001 aaccgtagct acctgacggt cgccattggt tgtaccggcg ggaagcaccg ttcggtgtat
    75061 attgcagagc aactggcaga ctacttccgc tcgcgcggta aaaacgtcca gtcacgccat
    75121 cggacgctgg aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc acaaacaagc
    75181 tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt tttgacgctg
    75241 aagtgctgtt acgtaatgac gaaggcaccg aggctgaagc caacagcgtt attgcgctgc
    75301 tgatgttgga ttctgccaaa ggacggcaga ttgaagttga agcgaccggt ccacaggaag
    75361 aggaagcact ggccgccgtt atcgccctct ttaactctgg ttttgatgaa gattaatctt
    75421 catcactttg acattcaaat cccttcaagc tcccccggca ataagaaaag cgcgtactga
    75481 aatcgttatc aatcaacgtc gattgtacat ttcgtgcgct tttttacttt cagaagaacc
    75541 ttaagaaaac cttaagaagc attgtttaga tttcgtttaa gttaatcgac catactggag
    75601 atcgtcagaa aatatttcca ggagatagca tgattcgctt atctgaacaa agtcccctgg
    75661 gcaccggaag acatcggaaa tgttatgcgc atccggaaga tgcccaacgc tgtatcaaga
    75721 ttgtctacca tcgtggcgat ggtggcgata aagagatccg ccgtgagtta aagtactacg
    75781 cgcatcttgg tcgccggtta aaagactgga gtggaatacc gcgctatcac ggtaccgttg
    75841 agacggattg cggaaccggt tatgtctacg atgtgattgc cgatttcgac ggcaagcctt
    75901 ccattacgct gaccgaattt gcagaacaat gccgttacga agaagacatt gcacaactgc
    75961 gccagttact gaaacagctg aagcgttatt tgcaggataa ccgtatcgtg acgatgtcgc
    76021 tgaagccgca gaatatcctc tgtcaccgca tcagtgagtc agaggttacc cccgtcgtct
    76081 gcgacaacat tggtgaaagc acattgattc cactggcgac ctggtcgaag tggtgttgtc
    76141 tgcgtaagca agagcggtta tggaaacgat ttatcgccca accggcgctg gctattgccc
    76201 tgcagaaaga tctgcaacct cgtgagagca aaacgctggc cctcacttcg cgcgaagctt
    76261 aatcaagctg gtgttgttgc ataaacggct caccgcctaa ctgatacatc tgccgtaaaa
    76321 tccacgcctg acggctacgc acgtagcccg atggcgcgga gactttgaaa cgaagtggat
    76381 taggtaatac agctgccagc aatgcagctt ccgaccgggt aagtttgctc gcgggtttgt
    76441 ggaaataacg ttgtgccgca gcttcgacgc caaacacacc gtcgccaaat tcggcgatat
    76501 tcaggtaaac cgtcaggata cgctttttgc tccagactgt ttctatcccc agcgttaatc
    76561 cggcttccag cccttttcga acccagctac gcccatccca taaaaagaga tttttggctg
    76621 tctgctgaga aatcgttgaa gcaccgcgaa tacggttttc attgcgctcg ttgtgcgcca
    76681 gggcttgctc aatggaagcg acatcaaagc cccagtgctc aggaaatttc tgatcttctg
    76741 cggcaatcac cgccagtccc atccacggcg agatttgatc catactgacc cagtcagaat
    76801 gtgccacgta acgaaaattg ccatgcagcc aggcgctgac ttgccgctcg accattaccg
    76861 ctgagaaagg aacaggcgca acgctaaaca acgcgatgcc cccgccccag aaaatggcga
    76921 ggacaaccat taaccgcaga agaaaacggc gaacgaaact aaacaccgtt aagcggcttt
    76981 tactcattca gccagaacca gcacgcggga aaccagctta tcaatgccgc tcgccgcttc
    77041 tgcaatgttc tgcgccagca tatatgccgg ggtggtgaca atcttattgt cttcatcaac
    77101 cacgatatca tcgacaggac acggcacatg ctccgcgccc atctcttcca gcacttctgc
    77161 ggtatcgata tcagtaccga tggtcaaacg cagcgggaaa tcgaaaattt tcggcagtat
    77221 cgccggtgct atacacataa aaccaagcgg ttttccggcc tgatgcatcg cttgtgccag
    77281 cgcctttaat tcacggtcaa cggtgcattc gctaccaaga ctggcaaaat tgcttaaatt
    77341 cttcgccgcg ccaaaacccc ccggcacaat caacgcatcc agttcagcgg catcggcctg
    77401 ggccagagga cggatttcac cgcgcgttat tcgtgccgct tcaatcagca cattgcgcgt
    77461 ttccgtcatc gcttcaccag ttaaatggtt gataacatca acctgctgct tatccggtgc
    77521 aaagcagacc gcctgcgcac cgctgcgtga aatagctaac aacgtcaaca ccgcttcatg
    77581 aatttcagaa ccgtcataga cgccgcatcc gctcagaatt acgccaattt tcttcattgt
    77641 gatcttcctt tcgcaattga ctgaaacaca ttaatttttt taataaaaat gctacgcatc
    77701 acacatttaa ctgattcatg taacaaatca tttaagtttt gctatcttaa ctgcgtgcgg
    77761 cctgaaaaac agtgctgtgc ccttgtaact catcataata atttatggcg cagccaagat
    77821 ttccctggtg ttggcgcagt attcgcgcac cccggtctag ccggggtcat tttttagtgg
    77881 cttttgccac ccacgctttc agtacttcta cgtcgtgacg ccactcttct ttcatctctt
    77941 caatccattc accgacgtta tcttcccagg cgggaaggtc aggagactga atttgctgac
    78001 ccagctgttg cagatggcgt aaccccactg accccgccgc acctttaatt ttatgccctt
    78061 cctcaacaat gcctttttta tcctgcgccg tcagattcga ctccagcacg ctgacatagc
    78121 ccggcatcat tctctcaaac accgctaacc cgtcggtgat cagcttcggt cctacaagtt
    78181 cgagatactg ttccagcatg ggaatatcga gcaatgcttc tgatttactg ttctcttctg
    78241 tcgtcaccgt actctcctca tcatcctggg tatcccagaa tttcttgatc atcgcggtta
    78301 gcgccggaac agaaagcggc ttactcagca catcatccat tccagcattg aggtactctt
    78361 gtttgtcttt cagcacgtta gcggttaagg ccaccagcgg cggtaaatcc tcgcgcggat
    78421 aacgcttcgt cagtgcacga gagatatcca acccggtcat atctggcaac tgaatatcca
    78481 gcaacaccag gtcgtattcg cccggtttaa acatctccag cgccgccttg ccggtcatgg
    78541 cgacatcaac gctgttacct aacttttcca gcacagagcg cgcgacaatc acgttcagtt
    78601 caatgtcttc caccagcagc acattcagcg ccggtaaagg catatcgtct tcatcaaacg
    78661 catcatcaac ctcttctgct accgacggtg cgtggatcgt caacgtaaag gttgaacctt
    78721 tgccctgttc gctggtaacc gtaatatcgc cgcccatatt tttcgccaga cgacgagaaa
    78781 cggccagacc aataccggtg ccggtggcag gtttaccgcc atgactgtct ttcacctggt
    78841 aatacatggc aaagatttta tccagctcat cctgcggaat gccgatacca gagtcttcca
    78901 cttcaaaatg cagcatatcg ccttcatcgt agcgcacgcg cacggtaacc tggccttgct
    78961 gagtgaattt gacggcgtta ctgatgaggt tccacaagat ctgccgtaaa cgcgtcccgt
    79021 cggtaatgac ctgatgcggt aatggcagcg tcggctccag attaaagcgc aatccttttt
    79081 gttgcgcctg caatgcggag agattttcca gatcggcaag gaagctggtg aaatcaaccg
    79141 gttgattatc aagctggacc ttgcgccgtt ccatcttatc catgtcgata atatcgttga
    79201 agatattccc cagcgtgaca gccgaaacat ggatggtttt gagatatttt tcctgctcgg
    79261 cggtgagttc agtatccagc agaatgcggc tcaggccgac gataccgttc agcggtgtac
    79321 gcaattcgtg actgatggtg gagataaacg tcgttttgtc gcggctggcg cgttcaagcg
    79381 catcctgata ccgcttacgc tcggtaatgt cgcgaccaaa gcccatcaaa ccgtgacgtt
    79441 tacccacgcg gtcgtagtac ggtactttac ggatttcaaa gcaggctttg cgtccgtcgg
    79501 gataatccag ccactgctca taggtcagtg acacattatg acggaacact ttttcatcgg
    79561 tttcaatgac tttcgcagcg gcttccggtg agtaaacatc agcaggtttc aggtgaacca
    79621 gttgtttttc gctttttccg gtcagcagct ccatcgcgcg gttacagccg gaaaactctt
    79681 tatcttcgtt acgataaaaa accaggtcgg gtgaagcgtc aaggaaggaa cgtaagaatg
    79741 aggattgctg ctcgagctga atttgtgtct cttcgcgctc tttgatttca attttcagtt
    79801 ggccgaaggt ttcctgcagt tctgcttccg ctttttcacg aacggcgatt tcctgattta
    79861 gctgggcaat attatctttt aactgaacgt tgaggctcaa atcgcgctcg cgcatctcct
    79921 ccagtttttg caccagtcgt gacagacgtt gtcgtgactc ctccagttgc tcgacgacca
    79981 ccgatagaaa gtagaccgcc cacggcgtaa tcagcaaacc aaagaagata gaacgaataa
    80041 catcaatgct ttcgacctgg ccatgcagca ccatggttac cgccatttgt accacaatgg
    80101 caagaacgac gagggccagc gccagcaaca ttgagaagcg caccagacct aacttcatca
    80161 tcaggtcaac ataatactgc gccagcagac gaatttgctt cattagggaa ttccttcacg
    80221 acaacctgcc ccaataatac ccaattctga cagttacgtt ataaattgtg tgagaaatgc
    80281 ggaattcccc tcaatggcct acgccgttgg aggtagccac ggacgtccca gcgccgatcc
    80341 ctgtacgcca tgttcattca gataacgatc cagcccgacc attcccgtcc atcgattttc
    80401 gcaccacagc ggagcaagca gcgtcgggcg acgggcgctg gcggaaatgc ggtggtagat
    80461 cacttccggt ggcgtatggc gaatcatttc tccggcagtg agcgtgtaat cctccagttc
    80521 aattccgttc aaacgccccg cttcccaggc tttcgccata atgctgcctt tcactatatg
    80581 cagcggatgc agctttatgc cgtccacgcc ggtttcaacc actcgctcca gcgtttgcaa
    80641 acattcagcc tgcccttcgc caggcaggcc aacaattaag tgagaacaca ccttcagccc
    80701 acgctcacgc gccagctgcg tcgtgcgctg atagcaggca aaatcatgac cgcgattgat
    80761 gcgatgcagt gttttgtcgt gggcggtttg tagccccagc tccagccaca cttcgtagcc
    80821 ctggtcctta tattcgcaaa gcagatccag caccgcatcc ggcacgcagt ccgggcgggt
    80881 accaacacac aaaccgacaa tattggcctg gctcaccgcc tgctgataca tcgaacgcag
    80941 cacctgaact tccgcaaagg tactggtata cgcctgaaag taggccagat agcgtttagc
    81001 gcggttaact aaattcgcct ggtgcgccag ttgctcggca atggaacgat gctgctgcgc
    81061 ttcatcggca aacgaggcaa cattacagaa tgtgcagccg ccacgcccga tggtaccgtc
    81121 acggttaggg cagctaaaac cgccatgcag cgtcagctta tgcacctttt gcccataacg
    81181 acgggtgaga tcaccaccaa acatattgac taatttctgt aactgcataa tctgatagac
    81241 cgcgccttga aaagaggcca aagcctgcca tttttagccc aattcggcga tgacctggat
    81301 caatcgtccc ggcctgcttt tatcaactgc ataatcaatc aaaattaccg aaatttcatg
    81361 cacaatcaca taaatcactt ttgcttatct tgtgtcagat ttttttatct ccttatggat
    81421 tttaggcaaa aacagtagca tgaaacgtta ttaccaatta aggcagtata aaatgctggt
    81481 tttctcgtca gttcaaggca ggataagggt taacacgcct ttatgacagt caggtattgg
    81541 ctgtttctct aacgacgccc cttttagcct taaatagaaa atccaattta atttcagata
    81601 attaataaag aattttgcgc taaagcacat ttctgtacca ataagcttgc catttgacct
    81661 gtatcagctt tcccgataag ttggaaatcc gctggaagct ttctggatga gcagcctgct
    81721 catcatattt atgcagtaat tgagatcccc tcttcaccgt attaaccgat gcgaaaagga
    81781 caacaagggg gcgaatgcga ggcgcgcgta tgacacgcaa accccgtcgc cacgctctta
    81841 ctgtgcccgt gcgcagcggt tcggaagtgg ggttcccgca gagcctgggg gaggttcacg
    81901 atatgttgta cgataaatcc cttgagaggg ataactgtgg tttcggcctg atcgcccaca
    81961 tagaaggcga acctagccac aaggtagtgc gtactgcaat acacgcactg gcccgcatgc
    82021 agcaccgtgg cgcgattctc gccgatggta aaaccggtga cggttgcggc ttgctgttac
    82081 aaaaaccgga tcgttttttt cgcatcgttg cgcaggagcg cggctggcgt ttagcaaaaa
    82141 actacgccgt cgggatgctc ttcctgaata aagatcctga actcgccgct gccgcacgcc
    82201 gcatcgttga agaagagctg caacgcgaaa cgttgtcgat tgtgggctgg cgtgatgtcc
    82261 ccactaacga aggcgtgctg ggtgaaatcg ccctctcctc tctgccacgc attgagcaaa
    82321 tttttgtgaa cgccccggca ggctggcgtc cgcgcgatat ggagcgccgt ctgtttatcg
    82381 cccgccgccg cattgaaaag cgtctcgaag ccgacaaaga cttctacgtc tgtagcctgt
    82441 cgaatctggt caatatttat aaaggtctgt gtatgccggc ggatctgccg cgcttctacc
    82501 tggacctcgc tgacctgcgt ctggaatcgg ccatctgcct gtttcaccag cgcttttcca
    82561 ctaacaccgt accgcgctgg ccgctggcgc agccgttccg ctacctcgcg cataacggtg
    82621 aaatcaacac catcactggt aaccgccaat gggcgcgtgc gcgtacctat aaattccaga
    82681 cgccgctgat ccctgacctg cacgacgccg cgccgttcgt caacgaaacc ggctctgact
    82741 ccagttccat ggataacatg ctggaactcc tgctggcagg cgggatggat atcatccgcg
    82801 ccatgcgtct gttagtacca cccgcctggc agaacaaccc ggatatggac ccggaactgc
    82861 gtgccttctt tgactttaac tccatgcata tggagccgtg ggatggcccg gcaggcatcg
    82921 tgatgtctga cggtcgtttt gccgcctgta acctcgaccg taacggtctg cgtccggcgc
    82981 gctacgtcat caccaaagat aagctcatca cctgcgcctc tgaagtcggt atctgggatt
    83041 atcagcctga cgaagtggtc gaaaaaggcc gcgtggggcc aggcgaactg atggttatcg
    83101 acacccgcag tgggcgtatt ctgcactcgg cagaaaccga tgacgatctg aaaagccgcc
    83161 atccatataa agagtggatg gagaaaaacg tccgccgact ggtaccgttt gaagatctgc
    83221 ccgatgaaga agtgggtagc cgcgaactgg acgacgacac gcttgccagc taccagaaac
    83281 agtttaacta cagcgcggaa gagctggatt cggtgatccg cgtactgggt gaaaacggtc
    83341 aggaagcggt cggttcgatg ggcgacgaca ccccgttcgc cgtgctctcc agccagccgc
    83401 gcattattta cgactacttc cgccagcagt ttgctcaggt aaccaacccg ccaatcgacc
    83461 cgctgcgtga agcgcatgtt atgtcgctcg ccaccagcat tggccgcgaa atgaacgtct
    83521 tctgcgaagc tgaaggccag gcgcaccgtt taagctttaa atcgccgatt ctgctctatt
    83581 ccgatttcaa acagctcacg acgatgaaag aggatcacta ccgcgcagat acgctggata
    83641 tcacctttga cgtcactaaa accacgctcg aagcgacggt caaagagctg tgcgacaaag
    83701 ccgaaaaaat ggtacgtagc ggcaccgtgc tgctggtgct ctccgaccgg aatatcgcga
    83761 aagatcgcct gccggttccg gctccgatgg cggttggcgc gatccagacc cgtctggtcg
    83821 atcaaagcct gcgttgcgat gccaacatca tcgtcgaaac cgccagcgcc cgtgatccgc
    83881 accacttcgc cgtgttgctg ggtttcggcg cgacggctat ttatccgtac cttgcctatg
    83941 aaacgctggg ccgtctggta gacacccacg cgattgccaa agattatcgt accgtgatgc
    84001 tcaactatcg taacggcatc aacaaaggct tgtacaaaat catgtccaaa atgggcatct
    84061 ccaccatcgc ctcttaccgc tgctcgaaac tgtttgaagc ggtcggtctg cacgatgatg
    84121 tcgtgggcct gtgcttccag ggggcggtta gccgcattgg gggggctagc tttgaagact
    84181 tccagcagga tctgctgaat ctgttgaaac gtgcctggct ggcgcgtaag cccatcagcc
    84241 agggcggcct gctgaaatac gtgcacggcg gcgaatacca cgcctacaac ccggacgtgg
    84301 tgcgcacgct gcaacaggcg gtacagagcg gcgagtatag cgactatcag gaatacgcga
    84361 agctggttaa tgagcgtccg gcaaccacgc tgcgcgatct gctggcaatt acgccgggtg
    84421 aaaacgcggt caacattgct gatgttgaac cggcaagcga actgtttaaa cgcttcgata
    84481 ccgccgcgat gtctatcggc gcgttaagcc cggaagccca cgaggcgctg gcggaagcga
    84541 tgaacagcat cggcggtaac tcaaactccg gcgaaggcgg tgaagacccg gcgcgctatg
    84601 gcactaacaa agtgtcgcgc atcaagcagg tggcgtccgg tcgcttcggc gttactccgg
    84661 cgtatctggt caatgccgac gtcattcaga ttaaagtcgc ccagggcgcg aagccgggag
    84721 aaggcggtca gttgccgggt gataaagtca ctccttacat tgccaaactg cgctattcgg
    84781 tgccgggcgt gacgctgatc tccccgccgc cgcaccatga tatctactct atcgaggact
    84841 tagcgcagct cattttcgac ctcaagcagg ttaacccgaa agcgatgatc tccgtgaagc
    84901 tggtgtccga accgggcgtc ggcaccatcg cgaccggcgt ggcgaaagct tatgcggact
    84961 tgatcaccat cgcaggctat gacggcggca ccggcgcaag tccgctttca tcggtgaaat
    85021 acgcaggctg tccgtgggag ctggggcttg ttgaaaccca acaggcgctg gttgccaacg
    85081 gcttgcgtca caagatccgt ttgcaggtcg atggcggcct gaaaacgggt gccgatatca
    85141 tcaaagcagc gattcttggc gcggaaagtt ttggcttcgg cactggcccg atggtagcgc
    85201 tcggttgtaa atatctgcga atttgccacc tgaacaactg cgccacgggt gtagcaactc
    85261 aggatgacaa actgcgtaag aatcactatc acggcctgcc gttcaaagtg acgaattact
    85321 ttgagtttat cgcccgtgaa acccgcgggc tgatggcaca gctgggcgta acacgtctgg
    85381 tggatctgat tggtcgcacc gacctgctga aagagctgga cggtttcacc gccaaacagc
    85441 agaaactggc gctgtcgaag ctgctggaga ctgccgagcc gcatccaggt aaggcgctct
    85501 actgcaccga aaacaacccg ccgtttgata acggcctgct gaacgcgcag ttgctgcaac
    85561 aggcgaaacc gtttgttgat gaacgccaga gcaaaacctt ctggttcgat atccgcaaca
    85621 ccgaccgttc tgtcggcgca tcgctttcag gctatatcgc ccagacgcac ggcgatcagg
    85681 ggctggctgc cgaccctatc aaagcgtact tcaacggcac cgcaggccag agcttcggcg
    85741 tgtggaacgc gggcggcgtg gaactgtacc tgaccggtga tgccaacgac tatgtcggta
    85801 aaggcatggc gggcggctta atcgccattc gtcctccggt tggctcagcc ttccgcagcc
    85861 atgaagcgag cattatcggc aacacctgcc tgtatggcgc gaccggtggt cgtctgtatg
    85921 ccgcaggccg cgcgggtgaa cgtttcggcg tgcgtaactc cggtgctatc accgtggtag
    85981 aaggcattgg cgacaacggc tgtgaatata tgacgggtgg tatcgtctgt attctgggta
    86041 aaaccggcgt taacttcggc gcgggcatga ctggcggctt cgcttacgtt ctcgatgaaa
    86101 gcagcgattt ccgcaaacgc gttaacccgg aactggtcga ggtcttaagc gttgacgatc
    86161 tggcgatcca tgaagagcat ctgcgtggtc ttatcaccga gcatgttcag cataccggtt
    86221 cccaacgtgg tgaagagatt ctggcgaact ggtcaacctt cgccactaaa tttgcgctgg
    86281 ttaaaccgaa gtccagtgat gtaaaagcac tgctgggtca ccgtagtcgt agcgcagcag
    86341 agttgcgcgt gcaggcgcag taaggggtag caacaatgag tcagaatgtt tatcaattta
    86401 tcgacctgca gcgcgttgat ccgccaaaga aaccgctgaa gatccgcaaa attgagtttg
    86461 ttgaaattta cgagccgttt tccgaaggcc aggccaaagc gcaggccgac cgctgcctgt
    86521 cgtgcggcaa cccatactgc gagtggaaat gcccggtaca caactacatc ccaaactggc
    86581 tgaagctcgc caatgagggg cgtatttttg aagcggcgga actgtcgcac cagacaaaca
    86641 ccctgccgga agtttgcgga cgtgtctgcc cgcaagaccg tctgtgcgaa ggttcctgca
    86701 ccctgaacga tgagttcggt gcggtgacca tcggcaacat tgagcgttat atcaacgata
    86761 aagcgttcga gatgggctgg cgtccggata tgtccggcgt gaaacagacc ggtaaaaaag
    86821 tagcgattat cggcgcaggt ccggcgggtc tggcgtgcgc ggatgtcctg acgcgcaacg
    86881 gcgtaaaagc gatcgtcttc gaccgtcatc cggaaattgg cggcttgctg accttcggta
    86941 ttccggcttt caagctggaa aaagaggtaa tgacgcgccg ccgtgaaatc ttcaccggca
    87001 tgggtattga attcaaactc aataccgaag tgggccgcga cgtgcagttg gacgatctgc
    87061 tgagtgatta cgatgccgtg ttccttggcg tcgggactta tcagtcaatg cgcggcgggc
    87121 tggaaaacga agacgccgat ggcgtgtacg cagcgctgcc gttccttatc gccaacacca
    87181 aacagttaat gggctttggt aaaacccacg acgaaccgtt cgtcagcatg gaaggcaaac
    87241 gcgtggtggt ccttggcggt ggcgacactg cgatggactg cgtgcgcacg tctgtacgcc
    87301 agggggcaaa gcacgttacc tgtgcctatc gtcgtgatga agagaacatg ccgggttccc
    87361 gccgcgaagt gaaaaacgcg cgggaagaag gcgtagagtt caaattcaac gtccagccgc
    87421 tgggtataga agtgaatggc aacggcaaag tcagcggcgt aaaaatggtg cgtaccgaaa
    87481 tgggcgaacc ggatgccaaa ggccgtcgcc gcgcggagat tgtggcaggt tccgaacata
    87541 tcgttccggc agatgcggtg atcatggcgt ttggtttccg tccacacaac atggaatggc
    87601 tggcaaaaca cagcgtcgag ctggattcgc aggggcgcat tatcgccccg gaagacaacg
    87661 acaacgcttt ccagaccagc aacccgaaaa tctttgctgg cggcgatatc gtccgtggtt
    87721 ccgatctggt cgttaccgcc attgccgaag gtcgcaaagc ggcggacggc attatgaact
    87781 ggctggaagt ttaagcgagg taacaataaa ttcgccagac agtgtctggc gaatttataa
    87841 aacgagcgat ccaactaaat tttaagccat taaatcagcc ttaaccttca ctaccacctt
    87901 tttaatctca ccaggttcgc caacaacgca tcccggttta tgcggttctc ctggcataaa
    87961 cacggcgaac attcccggtt tcaggatgat ggcttgctcg ttttcaatgg cgctgcaaag
    88021 ctgataatca tcttcatggt ggaactcttc acactgacgc gcagtgccag ccatgccaaa
    88081 cagaatccgt tcctcaccgt ttaataacag ctgaatatca atgtattgtt cgtgcaattc
    88141 ggcttttttc tcaacgggcg attgagtgtt aaacgtcatg acattcataa aaatattgtc
    88201 gccctgtaat tcgtaacggc caggcgcttt ttcttgcggt ctggcagcta atgccagcgt
    88261 taacgcgccc tgtaacgcag gatgtaatcc ggcagacggt aatgactgta cttcacccat
    88321 catcataata tttctccctg ggccaacagc gcagccccaa gtaaacctgt atcgtggcgg
    88381 tagtgcgccg ccagtaaatc aacatgaaat gccgctggct cctgcgccag atacgtttcc
    88441 accagcgcca gatacccttc tgccagacca acgctgccac cgaccaccac gcactggcaa
    88501 tcagttgtgg ctttaatatc agcgatcagc cttgcaagcg tacgtgcgga gcggtgaatc
    88561 agttgctgcg cctgctcatc gccctgcccg gcgcgcgtga aaatagtttt cgcattcgcg
    88621 ccagccaact ccccctgcgc tgccgctgca atgccgcgac cagaagcaat cgcttccacg
    88681 caacctgtgc gtccacagcc gcagactgga ccatgcggat cggcaagcgt atgcccgata
    88741 tgccccgcca gaccgccagg gccggtacgc agtttaccgc cgctcactac accgccgcca
    88801 acgccggtgg aaacagtgat aaacaccata tcagttatat cgccatccag cgcctgatac
    88861 tccgcccatg ctgcggcctg cgcatcatta atggcaatgg tcggcaaatt ggtaagttgc
    88921 tccagcgttt tgactaacgg aaagtgtaac aatccaccga gattatgcgg attcagcgcc
    88981 agcaagctgc cgtcgcgaat aattccggtt gaagcgatgg caacccgctg cgcatgtgct
    89041 tgcaacggag agactaatgc ggataaggca tcacgcagcg cctgcggagt ctggctggca
    89101 ggcgtaggaa gttctcggcg atcgcggatc tgcccgtcag cgccaatcag cgcggcggca
    89161 agtttagtac cgccgatatc aatcgccagt gtggtcatag caccgccttt ttcatcgctg
    89221 tgttgtacca ctgacaaatg tgttcaagac gcgtaatggc agaaccgacc gtcaccgccc
    89281 acgcgccgtg gcgcatcgca tccgccgcct gagcaggcgt gttgtaacgc ccttcggcaa
    89341 tcacccggca tccggcgtca ctcaacgttt tcaccagcgc cagatccggc tcttctggcg
    89401 tttcaggcgt ggtatagcca gaaagcgtag tgccaataat ttcggctccc agcttttggc
    89461 atgccaggcc gtcttccggc gttgagcagt cggtcatcgc cagtaaaccg tgatggtgaa
    89521 tacgtgccag cagcgtttca acaggtaccg gacgcggtcg gtcggtgccg tcaatggcga
    89581 taatatccgc gcccgcctgc gccagcgcat caacatcttc aatataggcc gtgatgcgta
    89641 ccggagaatc ctccagatcg cgcttcacaa tcccgataat cggcacgctc accaccgcac
    89701 gcgtggcttg cagatttgcc acaccttcaa tacgaatggc gaccgcgccc gcctgctctg
    89761 ccgctaatgc catggcggcg acgatttcgg gtttatcgag cgggctgtcc ggaaccggct
    89821 ggcaggagac aatcaggcca ccgttagcag cgattttttg atccagttgt gcaagtaacg
    89881 acatacatct tctcttagcg aaaggcccgg tacagagacc gggcaacagg attaactttt
    89941 ggttttgact aaatcgtttt tggcgctgcc aaacggcacg gcaccgctaa atggtttgcc
    90001 gtcgatagcg tcatgagtac gcaatgcttc cgggcgcagc caacgctgaa cacgagaagg
    90061 catatccaga ccaatcagca ggatcaccac aaacgttaaa ctaaacgaga gcgagccaag
    90121 tgccgttccc agatccagac gttgagcgat caacgcgccg aggattggag ctagtgcacc
    90181 gcccagtgcg ccaacgttgt aggtaaagcc cagccccgcc gcacgctggt cggtatcgaa
    90241 ataaccgccg atcagtttcg gtaaaatccc tgagatgccc tgaccaagca tttgctggaa
    90301 gaacagtaac agaccgagca cccagacgtt tgcgccgcca atcgcaaata ccggaataat
    90361 cagcagctgc gaggccagca gactacaaac gtacgctttg cgggttccca gccagtcacc
    90421 gaggaagcca ccgacgcagc accccaccgc cgcgccaaag ccactaaaga acagcacatt
    90481 ggcgacagta tgcgggtcat aagccagatc ggttttcaga tacgttggca gcagcgcctg
    90541 aatcggccat gagtagagga aagcaaacaa cacgaccacc atcagcatta cgcccgttgg
    90601 ccagcgtttg cccgtacttt gcaccataaa gctgataaag attgcggcgc ataacagccc
    90661 gagtacagcg acgatcgcgg cattttgcag gttaccggcg aagcagaacc acagcgcagt
    90721 agccgccgcc agtgtcatta cgatattggc aatgcgatgt tcaccacggt agagaatgtc
    90781 taccatcgtg cgtactggtg ctttaccccc gtgtttctct ttccagtctt ccgcttccgg
    90841 gatgttttta cgcagccaga gagcaaagat gattggcaaa atgccgataa agaacagcgc
    90901 tctccagccc cagaccggaa ccaccagact atagacctga gcggcaacga cggcccccac
    90961 tgagaagcct gaaatcagaa aaccactggc tttgttacgc agatgctttg gccagctttc
    91021 aatgacatag gtggcgctgg aaccgtattc acccgccatc cccatgccga tgaccagacg
    91081 agcgataaac atggtgatgt agcctggcgc aaagccgcag gccagcgtcc cggccgagaa
    91141 gagaacgatg ctggtgacca ttgccagacg acgcccgtag cggtcaccca tagcgccgag
    91201 catcaggccg ccgaaccagc gagagataaa ggctgcggag atcagacttg ccgcctgcac
    91261 cgtcgtcagc ccgaattcac cctgtacttc ggtgagtacc agggcgatta aaacgaaatc
    91321 aaaaccgtca agcagatatc ccaaccaggc agcggaaaat gcccgccatt gtgcgcggtt
    91381 aagatggcga taccacggga tattctgggt tgtagtactc attgtgagtc tcccgcggtg
    91441 ggcgatgccc acacgctttg gtatgaaaat tgtagggtac agatgcgttt atttcccctc
    91501 acccggtagg ggcgagcgag gggaaacaac tcacccgcgc tcttgcatca actgctgggc
    91561 cagcgccttc agttctggca gatatttttc atctaccgga ccaaacggtt tgcggcacag
    91621 cggcacagaa acgacatcca tataatgcag gacagttttc aggccgcgga atacgccagt
    91681 tttgatcagt aaatcaatga ctttgttgca ctcagtttgc agtttctgcg cggtctggat
    91741 atcgccttct ttcagcgcct tcacgatccc ctgataacgc cagcccatga tgttgtaagt
    91801 gctgccgata ccgccgtctg cgcccgccag aagaccagag gcgaagattt cgtcgtaacc
    91861 gttatagagc accagatcag gatgttcacg acggatctgc tccatctgat agagatcgcc
    91921 agaggtctgt ttcagcgcac ctacaccagg cagcgtaacc agggtgttga tctgatccag
    91981 ggtcagtttt accccgctca gggctggaat gttgtacacc accatcggca acccatccgc
    92041 cgaatcaata attgcccgat agtgatcgca gtgttcttca aagctgaaag gatagtagaa
    92101 cggcgtgacg gcggagacgg catcgaagcc ataacgttta gccgatgccg caagttgttg
    92161 gctttcggcg gtgctgacgc aaccgacgtg ggcgatgagt ttaatcttac ctttcgcctc
    92221 ttcggcgacg atttccagta cctgttcgcg ctcggaaagg ctttgtacaa aggcttcgcc
    92281 tgtcgaacca cccacgtaca aaccgtcgat gccctgctga atattgaact gaaccaggcg
    92341 gcgcagactc gctttatcca gtgcttgttg ttggtcaaaa ggagtcagga gtgcagccat
    92401 tacgccacgt aaattcgttg ccataaatac ctctgaagtg atgcttgtct gataaacgat
    92461 atacctttat acctgttata ccagatcaat taagcaacac cccatacaga aagcttataa
    92521 tgcgatctgc ttcactaaag tggcattatt tctttttgtt ggtggtctga ccgaaggcgt
    92581 gccaggtagc agagacgctg ttgagatgcg attgcaacgc gcgatcggct tcgtcaggat
    92641 catgacggcg gatcgcgtca acgatcgcaa tatgctgttg atagctaacg ttgttatgct
    92701 catgcagagc ctgatcagcc acggttggac gtgcggcgat tagccagtcg agcagggcaa
    92761 cgtggatcgc catgaagatt gggttaccag ggatctccgc cagaacgcgg tggaaatcaa
    92821 cgtctgagcg aatgaatgcc gcgttgttat ccagcgactg actgttgatt tccagtgctt
    92881 ttgccagcaa atcgatttgc tcatcggtgg cgtgttcagc cgcatagcgc accaggctgg
    92941 attcaaagaa cagacgtaat tgttcgaaat gggcaatccc accaggatga gaaaggaaat
    93001 ctttcgccat gccggaaagc tcgccgatga tggtgtccgc agaaggacgt gagacgcggg
    93061 cacgttcgcc gttgtttatt tgcaccagac ctttgcgttt taacgctgcc agcgcttcgc
    93121 gcaccgaagg acgcccgacg ttaaagaacg ccatcagttc gcgttcagac ggtaattgtt
    93181 caccttcacc aaattcacga cggcggatca tctgttccag ctcttcttcc accatttcgg
    93241 agagtttttt acgcgccagc gggcggctgc gcaagttgcg cccgatagtg gtagtagaat
    93301 cttcagcttg cggatcaaat gcgttcatag gactcattca ggaaaatgcg ttagcggata
    93361 ataacgacgc tatcgtagca cagtatcaaa gtcaggcgaa aagatgacag atagcaaaac
    93421 aggccacatg ggcctgtttt cattttactt cacaacgcgt aatgccggtc gaccaccgcg
    93481 tggtggctgc ggaggttcat cgtcaggatg agtttcatca tcgtgatctg gcttgtcgcc
    93541 atcgataacc gacataacgg tttcgttgtc tgccgatgcc tcttcatcat tcatgatgct
    93601 ggcatcttca tcgtaggcag cttcaggctc aaacatcgtg cctgcgccat tttcacgggc
    93661 gtagatagct agcacagcag ccagcggcac agaaacctga cgcggaatgc caccaaagcg
    93721 cgcgttaaag cgcacctcat cattcgccag ttccagattg ccgacagcac gcggcgcaat
    93781 gttgagtacg atttgcccgt cacgcgcata ttccatagga acctgcacgc cagggagcgt
    93841 cacatccacc accaggtgcg gcgtgagctg gttatccagc aaccactcat agaatgcacg
    93901 cagcagatag ggacgacgtg gtgttagctg tgacaaatcc atacagatta actccggccc
    93961 agacgcattt cacgttctgc ttcagttaaa gaagcaagga aagagtcacg ctcaaatacg
    94021 cgggtcatat agcctttcag ctctttcgca cccgggccgc tgaactcgat gcccagttgc
    94081 ggcagacgcc acagcagagg agcaaggtag caatcgacca ggctgaactc atcgctcagg
    94141 aagtacggct tctgaccgaa gaccggcgca atcgccagca gttcttcgcg cagttgctta
    94201 cgtgcggcat cggcttcaga agctgaaccg ttgatgatgg tgttcatcag cgtgtaccag
    94261 tctttttcga tgcgatgcat gtacagacgg ctttcaccgc gagctaccgg gtaaacaggc
    94321 atcagtggcg gatgcgggaa acgctcatcc agatattcca taatgatgcg agattcccac
    94381 agggtcagct cacgatccac cagggtcgga acgctctgat tcgggttgag gtcaatcaga
    94441 tcctgaggcg gattgtcctt ttccacgtgt tcgatctcga aacttacacc tttctcagcc
    94501 agcacaatgc ggacctgatg gctatagatg tcagtaggac cggaaaacag cgtcattacc
    94561 gaacgtttgt tggcagcgac agccatgaaa acctccaggt atagtcagaa tttttactgc
    94621 taccagccac caggtggcca gtcagaagtt gtgttaccca gtaaggaacg actctctttg
    94681 ttcgaaaatc aaacaaaaaa tgagtaatac ccgacatttg ggcagaaaat tggatgatag
    94741 tttaccagat tttgcgacct ttgtggtgag tcgatgccgg aaatggggaa aaagagatgc
    94801 gctttagtct gaaatagttg acttagtccc ttattggcga tgtggttttt gttttacctg
    94861 tctgtcaggt ggcagcaaaa agcaactttc cattttttac gctgatgcag attttagcca
    94921 taaaaaaacc cgccgaagcg ggttttttcg caaattgttt tctgccggag cagaagccaa
    94981 ttaacgtttg gagaactgcg gacgacgacg tgctttacgc agaccgactt tcttacgttc
    95041 aacctgacga gcgtcacgag taacgaagcc agctttacgc agttcagaac gcagggactc
    95101 gtcgtattcc atcagagcgc gggtgatacc gtgacggatc gcaccagcct gaccagagat
    95161 accaccacct ttaacggtga tgtacaggtc cagtttctca accatgtcga ccagttccag
    95221 cggctgacga actaccatgc gagcagtttc acgaccgaag tactgttcca gagaacgttg
    95281 gttgattacg attttaccgt tgcccggttt gatgaaaacg cgagctgcgg aacttttgcg
    95341 gcgaccagtg ccgtagtatt gattttcagc cattgcctat aatcccgatt agatgtcaag
    95401 aacttgcggt tgctgtgccg cgtggttgtg ctcgttaccc gcgtaaactt tcagtttacg
    95461 gaacatagca cgacccagcg ggccttttgg caacatgcct ttaaccgcga tttcaatcac
    95521 acgctcagga cggcgagcaa tcatctcttc aaaggtcgct tgtttgatac caccgatgtg
    95581 gccggtgtgg tgatagtaca ctttgtcagt acgcttgttg ccggttacag caactttgtc
    95641 agcgttcagt acgatgatgt aatcaccggt atctacgtgc ggagtgtatt ccgctttgtg
    95701 cttaccgcgc aggcgacgag ccagttcagt agccagacgg cccagagttt taccggtcgc
    95761 gtcaacaaca taccagtcgc gttttacggt ttctggttta gctgtaaaag ttttcattaa
    95821 aagcttaccc aataaatagt tacacgttgg tgaacaccca aacgtcttca attgttgagg
    95881 ttcacacgac aaagtccggc aaacctaccc cttcgaatag cctatgccag cacacaaaaa
    95941 gtttcgggaa aaaaaacttt cttgtaacgt ggggtcgcag gattatagag aagtcggggt
    96001 caaagatcga cccctttttg tgatttgtgg caggttttac cccgccaaat gctcgcgctt
    96061 caaatactct tcgctttgca tctcttgcag acgtgacagg caacgctgga actcaaactt
    96121 cagccgctcg ccctgataaa tttcatacag cggcacttct gcactcacca ctaatttgac
    96181 atggcgctcg taaaactcat ccaccagcgc aataaagcgt cgcgcttcgc tctccatcaa
    96241 ccgcgtcata actggtacat caaacaacat gaccgtgtga aagaggcgtg agagcgcaat
    96301 atagtcatgc tgactgcggg cgtcgacgca cagcgtagta aaagaaaccg ccagcgtctg
    96361 gttctcgacg cccattgtcg ccaatggccg atggttgatt tctaacgtcg gtgaattttc
    96421 tcgtttcgcc cccgccagcg ccaaccatag tttatccatt tgcgcccgga tttcatcgtt
    96481 gagtggcgaa agccacaggt gcgcctgagt gagtgtacgc aggcgataat caacaccagc
    96541 gtccacgttc attacatcac aatgctgttt aatggcatcg attgcaggca aaaaacgcgc
    96601 acgttgcagg ccatttcgat aaagttcgtc cggcggaata tttgacgtcg ctaccagggt
    96661 aataccgcgg gcgaacaggg ctttcatcag accgccaagt agcatggcat cggtaatatc
    96721 agaaacaaaa aattcgtcaa aacagagcac gtcagtttct gctttaaagc gatcggcgat
    96781 aatttccagc ggatcgcttt ggccttgtaa ttcggtcagc tcatcgtgca cccgcagcat
    96841 aaaacggtga aagtgcaggc gctgtttccg ctctcccggc agactttgat agaaaaggtc
    96901 catcagccag gttttcccgc gtcctacacc gccccacata tataagccac gcactggcat
    96961 atgctttgtg tcttcgcgtt taccccacag cttaccgacc cgcgccatta gcccactcgt
    97021 cctgggggct ggtggcgtgc tattgatgag ttcctgataa ataatttcca ggcggctgac
    97081 ggcctctttt tgaacgtcgt cgggttgatg actgccttca ttgagcgcct tcaggtattg
    97141 cgaggttggg gtaacgcttt gcatgatctt attgttattc cttgaataat cggtgcgccg
    97201 ttgttcacgg ttgacgaaaa aaaggccgtt ctacactacg cgatatgctg tcgggattcc
    97261 acttctgtgg aattaacggt tatagtggca taatcagccg caggcatgga gcctgaagcc
    97321 aacaccctac ggaaacaaaa gacaacggga gatgttcatg acctgggaat atgcgctaat
    97381 tgggttagtc gtcggcatca ttattggtgc tgtggccatg cgttttggta atcgtaaact
    97441 acgccagcaa caggcgttgc agtacgaact ggaaaagaat aaagctgaac tggacgagta
    97501 tcgcgaagag ctggttagcc actttgcccg cagcgcggaa ttactggata ccatggcgca
    97561 cgactatcgc cagctgtatc agcacatggc gaaaagctcc agtagcctgc tgccggaact
    97621 gtctgcggaa gcaaacccgt tccgtaatcg tctggcagag tctgaagcca gcaacgatca
    97681 ggcaccggtg cagatgcctc gcgactattc tgaaggcgca tccggcctgc tgcgtactgg
    97741 cgcgaagcgc gactaattta tttttcgggc gcagccattg cgccctcctc ttctctccct
    97801 ccccgactat catttaatct ggtgtctcat tgttagccgt ctgaaaattc aataacatca
    97861 aactgttttg aatctctttt cttatcattc aggtacgaga gcaggaataa tgaaaaaaca
    97921 aacccagctg ttgagtgcat tagcgttaag tgtcgggtta actctctcgg cgtcatttca
    97981 ggccgtcgcg tcgattccag gccaggttgc cgatcaggcc cccctcccca gtctggctcc
    98041 aatgctggaa aaagtgcttc cggcagtggt gagcgtacgg gtggaaggaa cggccagtca
    98101 gggacagaaa atcccggaag aattcaaaaa gttttttggt gatgatttac cggatcaacc
    98161 tgcacaaccc ttcgaaggtt taggctccgg tgtcatcatc aacgccaata aaggctatgt
    98221 gctgaccaac aaccatgtga ttaatcaggc acagaaaatc agtattcagc tcaatgatgg
    98281 gcgcgagttt gatgcaaaac taattggtag cgatgaccag agcgatatcg ccctgttgca
    98341 aattcaaaac ccgagcaaat taacgcaaat cgctattgcc gactccgata aattgcgcgt
    98401 cggtgatttt gccgtggcgg tcggtaaccc atttggcctt gggcaaaccg ccacctctgg
    98461 cattatatcc gcattaggcc gcagcgggtt gaatcttgaa ggtctggaaa actttatcca
    98521 gacagatgct tccattaacc gcggtaactc cggcggtgca ctgttaaacc ttaacggtga
    98581 gttaattggc atcaacactg caatccttgc gcctggcggc ggtagcgtcg ggattggatt
    98641 tgccattccc agtaatatgg cgcgaatact ggcgcagcag cttatcgact ttggagaaat
    98701 caaacgcggc ctgttaggta tcaaaggcac cgagatgagt gccgatatcg ccaaagcctt
    98761 caaccttgac gtgcagcgtg gcgcgtttgt cagcgaagtg ttgccaggtt ctggctcggc
    98821 gaaagcgggc gtcaaagcgg gcgatattat taccagcctc aacggcaaac cgctgaatag
    98881 ctttgctgag ttgcgctctc gtatcgcgac caccgagccg ggcacgaaag tgaagcttgg
    98941 cctgctgcgt aacggcaaac cactggaagt agaagtgacg ctcgatacca gcacctcttc
    99001 gtcggccagc gctgaaatga tcacgccagc gctggaaggt gcaacgttga gcgatggtca
    99061 gctaaaagat ggtggcaaag gtattaagat cgatgaggtt gtcaaaggaa gcccagctgc
    99121 tcaggctggc ttgcaaaaag acgatgtgat cattggcgtc aaccgcgatc gggtgaactc
    99181 gattgctgaa atgcgtaaag tgctggcggc aaaaccggcc atcattgccc tgcaaattgt
    99241 acgcggcaat gaaagcctct acctactaat gcgttaatgt cgtaaaccgg gcatcaggct
    99301 gacgtgtgat gtccggttaa ctcgtggtat gctgctgccg ttcccttttt taacgatgcc
    99361 tccatcatgt ttgtgaagct cttacgttcc gttgcgattg gattaattgt cggcgctatt
    99421 ctgctggttg ccatgccttc gctgcgcagc cttaacccgc tttccactcc gcaatttgac
    99481 agtaccgatg agacgcctgc cagctataat ctggcggttc gccgcgccgc gccagcggtg
    99541 gttaacgttt ataaccgcgg tttgaacacc aactctcaca accagcttga gatccgcacc
    99601 ctgggatctg gtgtaatcat ggatcaacgc gggtatatca tcaccaataa acacgtgatc
    99661 aacgacgccg atcagatcat cgtcgcctta caggatggac gtgtttttga agcgttactg
    99721 gttggctctg actctctaac cgatctggcg gtgctgaaaa ttaacgccac aggcggttta
    99781 ccaacgattc cgatcaatcc acgccgcgta ccgcacattg gcgatgtggt tctggcgatc
    99841 ggtaacccgt acaacctcgg gcagaccatt acccaaggga ttatcagtgc cacgggtaga
    99901 atcggtctga acccgaccgg gcggcaaaac ttcctgcaaa ccgatgcttc cattaaccac
    99961 ggtaactctg gcggcgcgct ggtgaactcg ctgggcgaac tgatgggcat taacacgctg
   100021 tcgtttgata agagtaacga tggcgaaacg ccggaaggta tcggctttgc gattcctttc
   100081 cagttagcga ccaaaattat ggataaactg atccgtgatg gccgtgtgat ccgtggctac
   100141 attggcattg gtggacgtga gatcgcccca ttacatgcgc agagtggcgg catggatcaa
   100201 ctgcaaggga tcgtggttaa cgaagtgtcg cctgatggcc cagcggcaaa tgcaggcatc
   100261 caggtcaacg atctgattat ctctgtgaat aacaaacccg ctatttccgc cctggagaca
   100321 atggatcagg tcgcggaaat tcgccctggt tcggtgatcc cggttgtcgt gatgcgtgac
   100381 gataaacagt taacattgca ggtcaccatt caggaatatc cggcgaccaa ctaaggtcag
   100441 cgtaaaaaaa caaaaaccgg agtctgtgct ccggtttttt attatccgct aatcaattac
   100501 ttattaacga actcttcgcc cagggcgata tctttcttca gcgtatccag cataccttcc
   100561 agcgcgttct gttcaaatgc gctcagagta ccgatagatt tacgctcttc cacgccgttt
   100621 ttacccagca gcagcggttg agagaagaaa cgagcgtact gaccgtcgcc ttcaacgtag
   100681 gcacattcga caacgccttg ttcgccctgc agtgcgcgaa ccagagacag accaaaacgt
   100741 gcagctgcct ggcccataga cagggttgca gacccgccac cggctttcgc ttcaaccacc
   100801 tcagtacccg cgttctggat acgtttggtc agatcagcca cttcctgctc ggtaaaacta
   100861 acgccaggaa cctgtgacag cagcggcaga atggtaacac cagagtgacc accaataacc
   100921 ggcacttcaa cttcgcctgg ctgtttgcct ttcagttccg caacaaaggt gttggaacga
   100981 atgatatcca gcgtggtaac gccgaacagt ttgtttttgt cataaacacc ggcttttttc
   101041 agcacttcag cagcaatcgc aactgtagtg ttaaccgggt tagtgataat accaatgcac
   101101 gctttcgggc aggttttcgc aacttgctgt accaggtttt tcacgatgcc ggcgttaacg
   101161 ttaaacaggt cggaacgatc catacccggt ttacgcgcta cgcctgcaga gataagaacg
   101221 acatctgcac cttccagcgc cggagtcgca tcttcacctg aaaaaccttt gattttcaca
   101281 gcagtaggga tatggctcag atcgacagcc acaccgggag tcactggagc gatatcatac
   101341 agagagagtt ctgaacctga aggcagttgg gtttttaaca gtagtgcaag cgcctggcca
   101401 ataccgccag cagcgccgag gactgcgact ttcatcctaa actccttatt atattgataa
   101461 actaagatat attgctccgc tgccgcgacc ttaatccaca aaattgccgt ttacaatgac
   101521 cacatctcaa gaatgtgtag tcacgcaagt ttagcgttta tgcatttaat tgccgtaatc
   101581 aggaacctaa cttacgtaat taacagtcac ttatgcatta gcgcaacatt ccaacaggtg
   101641 gtgacaatat accctaccgc tcagccaaaa caatatcaat ttgataacaa ttaatttact
   101701 tttaagcaga atttgcaggc cgtgacgcag gcatgtttct caataacgaa atttgataaa
   101761 atcccgctct ttcataacat tatttcagcc ttcttcaggg ctgactgttt gcataaaaat
   101821 tcatctgtat gcacaataat gttgtatcaa ccaccatatc gggtgactta tgcgaagctc
   101881 ggctaagcaa gaagaactag ttaaagcatt taaagcatta cttaaagaag agaaatttag
   101941 ctcccagggc gaaatcgtcg ccgcgttgca ggagcaaggc tttgacaata ttaatcagtc
   102001 taaagtctcg cggatgttga ccaagtttgg tgctgtacgt acacgcaatg ccaaaatgga
   102061 aatggtttac tgcctgccag ctgaactggg tgtaccaacc acctccagtc cattgaagaa
   102121 tctggtactg gatatcgact acaacgatgc agttgtcgtg attcatacca gccctggtgc
   102181 ggcgcagtta attgctcgcc tgctggactc actgggcaaa gcagaaggta ttctgggcac
   102241 catcgctggc gatgacacca tctttactac ccctgctaac ggtttcaccg tcaaagagct
   102301 gtacgaagcg attttagagc tgttcgacca ggagctttaa tctctgcccc gtcgcttctg
   102361 gcggcgggga aaatgttgct tttgcctatc aaccccctgc tttcccctgc gattcattta
   102421 acgaatagtg cgttttactg cgacatatca ttcacacaat gaatacacat agtaaaaaag
   102481 cacattatgc aaaattcatt atc
//
LOCUS       DAZFFE010000004        96079 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFFE010000004 DAZFFE010000000
VERSION     DAZFFE010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 96079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.004"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            400..855
                     /gene="csgB"
                     /locus_tag="ACHJ69_000334"
     CDS             400..855
                     /gene="csgB"
                     /locus_tag="ACHJ69_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO5013405.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            896..1354
                     /gene="csgA"
                     /locus_tag="ACHJ69_000335"
     CDS             896..1354
                     /gene="csgA"
                     /locus_tag="ACHJ69_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO5013406.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            1412..1744
                     /gene="csgC"
                     /locus_tag="ACHJ69_000336"
     CDS             1412..1744
                     /gene="csgC"
                     /locus_tag="ACHJ69_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO5013407.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQLIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPSSSEKS"
     gene            1865..2176
                     /gene="ymdA"
                     /locus_tag="ACHJ69_000337"
     CDS             1865..2176
                     /gene="ymdA"
                     /locus_tag="ACHJ69_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO5013408.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFNGQIVEPACDV
                     STQSSPVEMNCPQNGSVPRKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            2271..2804
                     /gene="ymdB"
                     /locus_tag="ACHJ69_000338"
     CDS             2271..2804
                     /gene="ymdB"
                     /locus_tag="ACHJ69_000338"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO5013409.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            2746..4227
                     /gene="clsC"
                     /locus_tag="ACHJ69_000339"
     CDS             2746..4227
                     /gene="clsC"
                     /locus_tag="ACHJ69_000339"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO5013410.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPQLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDNILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHDDAMTHRYLRKMESSPFINHLVDGTLSLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHSKKEIILKKEPATSFWKRVMVKLASILPVEWLL"
     gene            complement(4235..5392)
                     /gene="mdoC"
                     /locus_tag="ACHJ69_000340"
     CDS             complement(4235..5392)
                     /gene="mdoC"
                     /locus_tag="ACHJ69_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO5013411.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            5786..7321
                     /gene="mdoG"
                     /locus_tag="ACHJ69_000341"
     CDS             5786..7321
                     /gene="mdoG"
                     /locus_tag="ACHJ69_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO5013412.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            7314..9857
                     /gene="mdoH"
                     /locus_tag="ACHJ69_000342"
     CDS             7314..9857
                     /gene="mdoH"
                     /locus_tag="ACHJ69_000342"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO5013413.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            10029..10256
                     /gene="yceK"
                     /locus_tag="ACHJ69_000343"
     CDS             10029..10256
                     /gene="yceK"
                     /locus_tag="ACHJ69_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO5013414.1"
                     /translation="MRLIVMSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDVHHGPYE"
     gene            complement(10257..10631)
                     /gene="msyB"
                     /locus_tag="ACHJ69_000344"
     CDS             complement(10257..10631)
                     /gene="msyB"
                     /locus_tag="ACHJ69_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFO5013415.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAENANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQDENTLHEWDEGE
                     FQLEPPLDTEEGRTAADEWDER"
     gene            complement(10714..11940)
                     /gene="mdtG"
                     /locus_tag="ACHJ69_000345"
     CDS             complement(10714..11940)
                     /gene="mdtG"
                     /locus_tag="ACHJ69_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO5013416.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQISN"
     gene            complement(12112..13032)
                     /gene="lpxL"
                     /locus_tag="ACHJ69_000346"
     CDS             complement(12112..13032)
                     /gene="lpxL"
                     /locus_tag="ACHJ69_000346"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO5013417.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIILPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            13257..14309
                     /gene="trhO"
                     /locus_tag="ACHJ69_000347"
     CDS             13257..14309
                     /gene="trhO"
                     /locus_tag="ACHJ69_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO5013418.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKVTRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPLFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPEPTE"
     gene            complement(14351..14926)
                     /gene="yceI"
                     /locus_tag="ACHJ69_000348"
     CDS             complement(14351..14926)
                     /gene="yceI"
                     /locus_tag="ACHJ69_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO5013419.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IELKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(14930..15496)
                     /gene="yceJ"
                     /locus_tag="ACHJ69_000349"
     CDS             complement(14930..15496)
                     /gene="yceJ"
                     /locus_tag="ACHJ69_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO5013420.1"
                     /translation="MSFTNTPERYGVISAALHWISAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            complement(15757..15870)
                     /locus_tag="ACHJ69_000350"
     CDS             complement(15757..15870)
                     /locus_tag="ACHJ69_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO5013421.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            complement(15918..17036)
                     /gene="solA"
                     /locus_tag="ACHJ69_000351"
     CDS             complement(15918..17036)
                     /gene="solA"
                     /locus_tag="ACHJ69_000351"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO5013422.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQMLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGVYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            complement(17151..17405)
                     /gene="bssS"
                     /locus_tag="ACHJ69_000352"
     CDS             complement(17151..17405)
                     /gene="bssS"
                     /locus_tag="ACHJ69_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO5013423.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            complement(17695..17940)
                     /gene="dinI"
                     /locus_tag="ACHJ69_000353"
     CDS             complement(17695..17940)
                     /gene="dinI"
                     /locus_tag="ACHJ69_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO5013424.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            complement(18014..19060)
                     /gene="pyrC"
                     /locus_tag="ACHJ69_000354"
     CDS             complement(18014..19060)
                     /gene="pyrC"
                     /locus_tag="ACHJ69_000354"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO5013425.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NQAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELDRGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            complement(19166..19726)
                     /gene="yceB"
                     /locus_tag="ACHJ69_000355"
     CDS             complement(19166..19726)
                     /gene="yceB"
                     /locus_tag="ACHJ69_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5013426.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     NGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            complement(19860..20507)
                     /gene="grxB"
                     /locus_tag="ACHJ69_000356"
     CDS             complement(19860..20507)
                     /gene="grxB"
                     /locus_tag="ACHJ69_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO5013427.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            complement(20571..21779)
                     /gene="mdtH"
                     /locus_tag="ACHJ69_000357"
     CDS             complement(20571..21779)
                     /gene="mdtH"
                     /locus_tag="ACHJ69_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO5013428.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            22015..22599
                     /gene="rimJ"
                     /locus_tag="ACHJ69_000358"
     CDS             22015..22599
                     /gene="rimJ"
                     /locus_tag="ACHJ69_000358"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO5013429.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVYDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            22610..23257
                     /gene="yceH"
                     /locus_tag="ACHJ69_000359"
     CDS             22610..23257
                     /gene="yceH"
                     /locus_tag="ACHJ69_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO5013430.1"
                     /translation="MKYQLTVMEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVENPPAVTDMSNAADGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            23259..24182
                     /gene="yceM"
                     /locus_tag="ACHJ69_000360"
     CDS             23259..24182
                     /gene="yceM"
                     /locus_tag="ACHJ69_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO5013431.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATASSLRMDKHRTNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGNATLESGTLLTNESGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            24292..25827
                     /gene="murJ"
                     /locus_tag="ACHJ69_000361"
     CDS             24292..25827
                     /gene="murJ"
                     /locus_tag="ACHJ69_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO5013432.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(25867..26283)
                     /gene="flgN"
                     /locus_tag="ACHJ69_000362"
     CDS             complement(25867..26283)
                     /gene="flgN"
                     /locus_tag="ACHJ69_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO5013433.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITGKTQQLRQLNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(26288..26581)
                     /gene="flgM"
                     /locus_tag="ACHJ69_000363"
     CDS             complement(26288..26581)
                     /gene="flgM"
                     /locus_tag="ACHJ69_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO5013434.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            complement(26657..27316)
                     /gene="flgA"
                     /locus_tag="ACHJ69_000364"
     CDS             complement(26657..27316)
                     /gene="flgA"
                     /locus_tag="ACHJ69_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO5013435.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCDQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEASNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMISGQVVSGVVDADGNILIN
                     L"
     gene            27471..27887
                     /gene="flgB"
                     /locus_tag="ACHJ69_000365"
     CDS             27471..27887
                     /gene="flgB"
                     /locus_tag="ACHJ69_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO5013436.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTKHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSVLSGQIKGMMNVLQSGN"
     gene            27891..28295
                     /gene="flgC"
                     /locus_tag="ACHJ69_000366"
     CDS             27891..28295
                     /gene="flgC"
                     /locus_tag="ACHJ69_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO5013437.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            28307..29002
                     /gene="flgD"
                     /locus_tag="ACHJ69_000367"
     CDS             28307..29002
                     /gene="flgD"
                     /locus_tag="ACHJ69_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO5013438.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTALNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            29027..30232
                     /gene="flgE"
                     /locus_tag="ACHJ69_000368"
     CDS             29027..30232
                     /gene="flgE"
                     /locus_tag="ACHJ69_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO5013439.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDSLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTLDSSDP
                     TGTANPATTLVFNANGVLTSNPTANISTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            30252..31007
                     /gene="flgF"
                     /locus_tag="ACHJ69_000369"
     CDS             30252..31007
                     /gene="flgF"
                     /locus_tag="ACHJ69_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO5013440.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTANNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVVGEAGPIAVPEGEEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQARRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            31179..31961
                     /gene="flgG"
                     /locus_tag="ACHJ69_000370"
     CDS             31179..31961
                     /gene="flgG"
                     /locus_tag="ACHJ69_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO5013441.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            32014..32712
                     /gene="flgH"
                     /locus_tag="ACHJ69_000371"
     CDS             32014..32712
                     /gene="flgH"
                     /locus_tag="ACHJ69_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO5013442.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            32724..33821
                     /gene="flgI"
                     /locus_tag="ACHJ69_000372"
     CDS             32724..33821
                     /gene="flgI"
                     /locus_tag="ACHJ69_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO5013443.1"
                     /translation="MIKFLSALILLLVITAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            33821..34762
                     /gene="flgJ"
                     /locus_tag="ACHJ69_000373"
     CDS             33821..34762
                     /gene="flgJ"
                     /locus_tag="ACHJ69_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO5013444.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSERTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTSMIQQMKSISDKVSKTYSMNIDNLF"
     gene            34828..36471
                     /gene="flgK"
                     /locus_tag="ACHJ69_000374"
     CDS             34828..36471
                     /gene="flgK"
                     /locus_tag="ACHJ69_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO5013445.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAGAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNNGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            36483..37436
                     /gene="flgL"
                     /locus_tag="ACHJ69_000375"
     CDS             36483..37436
                     /gene="flgL"
                     /locus_tag="ACHJ69_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO5013446.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTESAPFSEADGKYEGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(37631..40816)
                     /gene="rne"
                     /locus_tag="ACHJ69_000376"
     CDS             complement(37631..40816)
                     /gene="rne"
                     /locus_tag="ACHJ69_000376"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO5013447.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAATATPASPA
                     QPGLLSRFFGALKALFSGGEETKPAEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTVETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAPAPRTELVKVPLPVVAQAAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQHEQEEVQVQPMVTEVPVAAAVKPVVSAPVVEEVAEVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEDTADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            41389..42348
                     /gene="rluC"
                     /locus_tag="ACHJ69_000377"
     CDS             41389..42348
                     /gene="rluC"
                     /locus_tag="ACHJ69_000377"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO5013448.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDGEFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            complement(42460..43044)
                     /gene="yceF"
                     /locus_tag="ACHJ69_000378"
     CDS             complement(42460..43044)
                     /gene="yceF"
                     /locus_tag="ACHJ69_000378"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO5013449.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            43243..43764
                     /gene="yceD"
                     /locus_tag="ACHJ69_000379"
     CDS             43243..43764
                     /gene="yceD"
                     /locus_tag="ACHJ69_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO5013450.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            43816..43989
                     /gene="rpmF"
                     /locus_tag="ACHJ69_000380"
     CDS             43816..43989
                     /gene="rpmF"
                     /locus_tag="ACHJ69_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO5013451.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            44070..45140
                     /gene="plsX"
                     /locus_tag="ACHJ69_000381"
     CDS             44070..45140
                     /gene="plsX"
                     /locus_tag="ACHJ69_000381"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO5013452.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            45208..46161
                     /gene="fabH"
                     /locus_tag="ACHJ69_000382"
     CDS             45208..46161
                     /gene="fabH"
                     /locus_tag="ACHJ69_000382"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO5013453.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            46177..47106
                     /gene="fabD"
                     /locus_tag="ACHJ69_000383"
     CDS             46177..47106
                     /gene="fabD"
                     /locus_tag="ACHJ69_000383"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO5013454.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIGKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYIAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            47119..47853
                     /gene="fabG"
                     /locus_tag="ACHJ69_000384"
     CDS             47119..47853
                     /gene="fabG"
                     /locus_tag="ACHJ69_000384"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO5013455.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAACGAKVIGTATSENGAQA
                     ITDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            48064..48300
                     /gene="acpP"
                     /locus_tag="ACHJ69_000385"
     CDS             48064..48300
                     /gene="acpP"
                     /locus_tag="ACHJ69_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO5013456.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            48388..49629
                     /gene="fabF"
                     /locus_tag="ACHJ69_000386"
     CDS             48388..49629
                     /gene="fabF"
                     /locus_tag="ACHJ69_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO5013457.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            49749..50558
                     /gene="pabC"
                     /locus_tag="ACHJ69_000387"
     CDS             49749..50558
                     /gene="pabC"
                     /locus_tag="ACHJ69_000387"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO5013458.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSITAYPAHYDRLRNEGMMLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDASFSSATLYEYLAPLCERPN"
     gene            50561..51583
                     /gene="yceG"
                     /locus_tag="ACHJ69_000388"
     CDS             50561..51583
                     /gene="yceG"
                     /locus_tag="ACHJ69_000388"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO5013459.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            51573..52214
                     /gene="tmk"
                     /locus_tag="ACHJ69_000389"
     CDS             51573..52214
                     /gene="tmk"
                     /locus_tag="ACHJ69_000389"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO5013460.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEVVMDAIRTTVTNWVKELDA"
     gene            52211..53215
                     /gene="holB"
                     /locus_tag="ACHJ69_000390"
     CDS             52211..53215
                     /gene="holB"
                     /locus_tag="ACHJ69_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO5013461.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVQSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            53226..54023
                     /gene="ycfH"
                     /locus_tag="ACHJ69_000391"
     CDS             53226..54023
                     /gene="ycfH"
                     /locus_tag="ACHJ69_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5013462.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            54318..55751
                     /gene="ptsG"
                     /locus_tag="ACHJ69_000392"
     CDS             54318..55751
                     /gene="ptsG"
                     /locus_tag="ACHJ69_000392"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO5013463.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(55811..57999)
                     /gene="fhuE"
                     /locus_tag="ACHJ69_000393"
                     /pseudo
     CDS             complement(55811..57999)
                     /gene="fhuE"
                     /locus_tag="ACHJ69_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            58334..58693
                     /gene="hinT"
                     /locus_tag="ACHJ69_000394"
     CDS             58334..58693
                     /gene="hinT"
                     /locus_tag="ACHJ69_000394"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO5013464.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLTHKGL"
     gene            58696..59073
                     /gene="ycfL"
                     /locus_tag="ACHJ69_000395"
     CDS             58696..59073
                     /gene="ycfL"
                     /locus_tag="ACHJ69_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO5013465.1"
                     /translation="MRKGCFGLMSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            59087..59728
                     /gene="lpoB"
                     /locus_tag="ACHJ69_000396"
     CDS             59087..59728
                     /gene="lpoB"
                     /locus_tag="ACHJ69_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO5013466.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            59709..60533
                     /gene="thiK"
                     /locus_tag="ACHJ69_000397"
     CDS             59709..60533
                     /gene="thiK"
                     /locus_tag="ACHJ69_000397"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO5013467.1"
                     /translation="MPFRSNNPLTRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENIDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            60544..61569
                     /gene="nagZ"
                     /locus_tag="ACHJ69_000398"
     CDS             60544..61569
                     /gene="nagZ"
                     /locus_tag="ACHJ69_000398"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO5013468.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            61592..62134
                     /gene="ycfP"
                     /locus_tag="ACHJ69_000399"
     CDS             61592..62134
                     /gene="ycfP"
                     /locus_tag="ACHJ69_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO5013469.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            62533..63837
                     /gene="ndh"
                     /locus_tag="ACHJ69_000400"
     CDS             62533..63837
                     /gene="ndh"
                     /locus_tag="ACHJ69_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO5013470.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            64064..64603
                     /gene="ycfJ"
                     /locus_tag="ACHJ69_000401"
     CDS             64064..64603
                     /gene="ycfJ"
                     /locus_tag="ACHJ69_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO5013471.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKA"
     gene            complement(64665..65297)
                     /gene="comR"
                     /locus_tag="ACHJ69_000402"
     CDS             complement(64665..65297)
                     /gene="comR"
                     /locus_tag="ACHJ69_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO5013472.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVASALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELLK"
     gene            65537..65794
                     /gene="bhsA"
                     /locus_tag="ACHJ69_000403"
                     /gene_synonym="ycfR"
     CDS             65537..65794
                     /gene="bhsA"
                     /locus_tag="ACHJ69_000403"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO5013473.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            complement(65877..66839)
                     /gene="ldtC"
                     /locus_tag="ACHJ69_000404"
     CDS             complement(65877..66839)
                     /gene="ldtC"
                     /locus_tag="ACHJ69_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO5013474.1"
                     /translation="MMIKTRFSRWLTFFTFAVAVALALPAKANTWPLPSAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(66982..70428)
                     /gene="mfd"
                     /locus_tag="ACHJ69_000405"
     CDS             complement(66982..70428)
                     /gene="mfd"
                     /locus_tag="ACHJ69_000405"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO5013475.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLINTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAIQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASNRGRYLMIGAAEHGFVDTMRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(70556..71629)
                     /gene="ycfT"
                     /locus_tag="ACHJ69_000406"
     CDS             complement(70556..71629)
                     /gene="ycfT"
                     /locus_tag="ACHJ69_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO5013476.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVICKIF
                     NRLALPLFVLFVLMSVAVNFVPTPWLGMNSVIRNLPYYSLGAWFGTTLMTCVKAVPLR
                     RHLLMASLLTVLAVGAWLFNISLLLSLVSIVVIMKLFYQYEQRFGMRSSSLLNVIGSN
                     TIAIYTTHRILVEIFSLSLIPQMNAVRWSPQVELTLLLIYPFVSLLICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPATVSYSR"
     gene            71890..73089
                     /gene="lolC"
                     /locus_tag="ACHJ69_000407"
     CDS             71890..73089
                     /gene="lolC"
                     /locus_tag="ACHJ69_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO5013477.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            73082..73783
                     /gene="lolD"
                     /locus_tag="ACHJ69_000408"
     CDS             73082..73783
                     /gene="lolD"
                     /locus_tag="ACHJ69_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO5013478.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFRVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            73783..75027
                     /gene="lolE"
                     /locus_tag="ACHJ69_000409"
     CDS             73783..75027
                     /gene="lolE"
                     /locus_tag="ACHJ69_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO5013479.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTDSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLKLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            75056..75967
                     /gene="nagK"
                     /locus_tag="ACHJ69_000410"
     CDS             75056..75967
                     /gene="nagK"
                     /locus_tag="ACHJ69_000410"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO5013480.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADRRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLAERLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            75983..76804
                     /gene="cobB"
                     /locus_tag="ACHJ69_000411"
     CDS             75983..76804
                     /gene="cobB"
                     /locus_tag="ACHJ69_000411"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO5013481.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQTFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(76942..77730)
                     /gene="ycfZ"
                     /locus_tag="ACHJ69_000412"
     CDS             complement(76942..77730)
                     /gene="ycfZ"
                     /locus_tag="ACHJ69_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFO5013482.1"
                     /translation="MEKIMILLSLLMFLPLTAISKPLIPIMKTLFTDVTGTVPDAEEI
                     AHKAELFRQQTGVAPFIVVLPDINNEASLRQNGKAMLAHAASSMSNVKGSVLLLFTTR
                     EPRLIMITNGQVESSMDDKHLGLLVENHTLAYLHADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENDPPGSTTTLGLFAWAVAFIVFAAFFNYTTRLYYALKFAVAMAV
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIATLLWTSKR"
     gene            complement(77715..78188)
                     /gene="ymfA"
                     /locus_tag="ACHJ69_000413"
     CDS             complement(77715..78188)
                     /gene="ymfA"
                     /locus_tag="ACHJ69_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFO5013483.1"
                     /translation="MSQDSRVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNLWVNFFKWGFIGIMGVIFIAVGLLISMPSSKKTRRKRKSRPWKKS"
     gene            complement(78246..79292)
                     /gene="potD"
                     /locus_tag="ACHJ69_000414"
     CDS             complement(78246..79292)
                     /gene="potD"
                     /locus_tag="ACHJ69_000414"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO5013484.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGSAS
                     SIYEEYYQKLKAGR"
     gene            complement(79289..80083)
                     /gene="potC"
                     /locus_tag="ACHJ69_000415"
     CDS             complement(79289..80083)
                     /gene="potC"
                     /locus_tag="ACHJ69_000415"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO5013485.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFIILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(80080..80937)
                     /gene="potB"
                     /locus_tag="ACHJ69_000416"
     CDS             complement(80080..80937)
                     /gene="potB"
                     /locus_tag="ACHJ69_000416"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO5013486.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            complement(80921..82057)
                     /gene="potA"
                     /locus_tag="ACHJ69_000417"
     CDS             complement(80921..82057)
                     /gene="potA"
                     /locus_tag="ACHJ69_000417"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO5013487.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            82307..83533
                     /gene="pepT"
                     /locus_tag="ACHJ69_000418"
     CDS             82307..83533
                     /gene="pepT"
                     /locus_tag="ACHJ69_000418"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO5013488.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLANMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(83582..84703)
                     /gene="roxA"
                     /locus_tag="ACHJ69_000419"
                     /gene_synonym="ycfD"
     CDS             complement(83582..84703)
                     /gene="roxA"
                     /locus_tag="ACHJ69_000419"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO5013489.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(84779..86239)
                     /gene="phoQ"
                     /locus_tag="ACHJ69_000420"
     CDS             complement(84779..86239)
                     /gene="phoQ"
                     /locus_tag="ACHJ69_000420"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO5013490.1"
                     /translation="MKKLLHLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(86239..86910)
                     /gene="phoP"
                     /locus_tag="ACHJ69_000421"
     CDS             complement(86239..86910)
                     /gene="phoP"
                     /locus_tag="ACHJ69_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO5013491.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHSEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(87079..88449)
                     /gene="purB"
                     /locus_tag="ACHJ69_000422"
     CDS             complement(87079..88449)
                     /gene="purB"
                     /locus_tag="ACHJ69_000422"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO5013492.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLEELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(88453..89094)
                     /gene="hflD"
                     /locus_tag="ACHJ69_000423"
     CDS             complement(88453..89094)
                     /gene="hflD"
                     /locus_tag="ACHJ69_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO5013493.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDGDALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGTLDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(89130..90236)
                     /gene="mnmA"
                     /locus_tag="ACHJ69_000424"
                     /gene_synonym="trmU"
     CDS             complement(89130..90236)
                     /gene="mnmA"
                     /locus_tag="ACHJ69_000424"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO5013494.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKDGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDADRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(90290..90751)
                     /gene="nudJ"
                     /locus_tag="ACHJ69_000425"
     CDS             complement(90290..90751)
                     /gene="nudJ"
                     /locus_tag="ACHJ69_000425"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO5013495.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(90761..91414)
                     /gene="rluE"
                     /locus_tag="ACHJ69_000426"
     CDS             complement(90761..91414)
                     /gene="rluE"
                     /locus_tag="ACHJ69_000426"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO5013496.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPDNQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVEEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGNYSLDNLANGEWRDATD"
     gene            91586..92836
                     /gene="icd"
                     /locus_tag="ACHJ69_000427"
     CDS             91586..92836
                     /gene="icd"
                     /locus_tag="ACHJ69_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO5013497.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(92950..94092)
                     /locus_tag="ACHJ69_000428"
                     /pseudo
     CDS             complement(92950..94092)
                     /locus_tag="ACHJ69_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(94082..94318)
                     /locus_tag="ACHJ69_000429"
     CDS             complement(94082..94318)
                     /locus_tag="ACHJ69_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO5013498.1"
                     /translation="MSRLITLQDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            complement(94458..94697)
                     /locus_tag="ACHJ69_000430"
     CDS             complement(94458..94697)
                     /locus_tag="ACHJ69_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5013499.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRAIRRK"
     gene            complement(94745..94963)
                     /locus_tag="ACHJ69_000431"
     CDS             complement(94745..94963)
                     /locus_tag="ACHJ69_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO5013500.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQWGL"
     gene            complement(95062..95277)
                     /locus_tag="ACHJ69_000432"
     CDS             complement(95062..95277)
                     /locus_tag="ACHJ69_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO5013501.1"
                     /translation="MTPQQENALRSIARQANYEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            complement(95354..95545)
                     /locus_tag="ACHJ69_000433"
     CDS             complement(95354..95545)
                     /locus_tag="ACHJ69_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO5013502.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLTTSL
                     SQKLEMMVAKAEADERDQV"
     gene            complement(95518..95700)
                     /locus_tag="ACHJ69_000434"
     CDS             complement(95518..95700)
                     /locus_tag="ACHJ69_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO5013503.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIQNPLKAQRLAEE
                     INNKRGAVCTKHLPLS"
     gene            complement(95697..>96079)
                     /locus_tag="ACHJ69_000435"
     CDS             complement(95697..>96079)
                     /locus_tag="ACHJ69_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HFO5013504.1"
                     /translation="TESPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRL
                     GGFEAIKSAYMAQVQYSMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEM
                     VPEFIEKMDEALAEIGFVFGEQWR"
BASE COUNT        23474 a        23679 c        25233 g        23693 t
ORIGIN      
        1 ctgtagtcct ttcgtcatga aaaacgttct tgttttttct ccataccacc gtggacaatt
       61 ttttactgca aaaagacgag gtttgtcacg gcttgtgcgc aagacatatc gcagcaatca
      121 gcgacgggca agaagaatga ctgtctggtg atttttgata gcggaaaacg gagatttaaa
      181 agaaaacaaa atatttttgc gtagataaca gcgtatttac gtgggtttta atactttggt
      241 atgaacaaaa aaaaatacaa cgcgcgggtg agttattaaa aatatttccg cagacatact
      301 ttccatcgta acgcagcgtt aacaaaatac aggttgcgtt aacaaccaag ttgaaatgat
      361 ttaatttctt aaatgtacga ccaggtccag ggtgacaaca tgaaaaacaa attgttattt
      421 atgatgttaa caatactggg tgcgcctggg attgcagccg cagcaggtta tgatttagct
      481 aattcagaat ataactttgc ggtaaatgaa ttgagtaagt cttcatttaa tcaggcagcc
      541 ataattggtc aagctgggac taataatagt gctcagttac ggcagggagg ctcaaaactt
      601 ttggcggttg ttgcgcaaga aggtagtagc aaccgggcaa agattgatca gacaggagat
      661 tataaccttg catatattga tcaggcgggc agtgccaacg atgccagtat ttcgcaaggt
      721 gcttatggta atactgcgat gattatccag aaaggttctg gtaataaagc aaatattaca
      781 cagtatggta ctcaaaaaac ggcaattgta gtgcagagac agtcgcaaat ggctattcgc
      841 gtgacacaac gttaatttcc attcgacttt taaatcaatc cgatgggggt tttacatgaa
      901 acttttaaaa gtagcagcaa ttgcagcaat cgtattctct ggtagcgctc tggcaggtgt
      961 tgttcctcag tacggcggcg gcggtggcaa ccacggtggt ggcggtaata acagcggccc
     1021 gaattcagag ctgaatattt accagtacgg tggcggtaac tctgctcttg ctctgcaagc
     1081 tgacgcccgt aactctgatc tgaccattac ccagcacggc ggcggtaatg gcgcagatgt
     1141 gggccaaggt tctgatgaca gctcaatcga tctgactcag cgtggtttcg gcaacagcgc
     1201 tactcttgat cagtggaatg gtaaagattc tactatgact gttaaacagt tcggtggcgg
     1261 taacggtgct gctgttgacc agactgcatc taactccagc gttaacgtca ctcaggttgg
     1321 ctttggtaac aacgcgaccg ctcatcagta ctaatacatc atcagtatga aagaaacagg
     1381 gcgcaagccc tgtttttttc gggagaagaa tatgaatgcg ttattactcc ttgcggcact
     1441 ttccagtcag ataaccttta atacgaccca gcaaggggat atgtacacca ttattcctga
     1501 agtcactctt actcaatctt gtctgtgcag agtacaaata ttgtccctgc gcgaaggcag
     1561 ttcagggcaa agtcagacga agcaagaaaa gaccctttca ttgcctgcta atcaactcat
     1621 tgctttgacg aagttgagtt taaatatttc cccggacgat cgggtgaaaa tagttgttac
     1681 tgtttctgat ggacagtcac ttcatttatc acaacaatgg ccgtcctctt cagaaaagtc
     1741 ttaatttgtt gaaatatcga gcataagatg aacctggaga gaatggtctg ctgcgaatca
     1801 gccaacctga aagtatggat aacacaaccc tcaaggatga ctaatcattg aggaaataga
     1861 ataaatgttc agaccttttt taaactctct tatgctcggc agtttgtttt ttccttttat
     1921 tgccattgca ggaagcaccg cgcaaggggg cgtgatccat tttaatggcc aaattgtgga
     1981 accggcatgt gacgtcagca cccagtcatc acccgtagaa atgaactgcc cacagaatgg
     2041 ttctgttccg cgtaaaacct attccagcaa agcgttaatg agcggcaatg ttaaaaacgc
     2101 gcaaatagcc tcagtaaaag tgcagtatct tgataaacag aaaaagctgg ctgtaatgaa
     2161 catcgaatat aactaagttt ctgaacaact cacgttgctg agcagaaaaa tgcgatttaa
     2221 ccaaaaagcc tgctgtacac ttaggaaaca agaaggtgta aggaggcgtt atgaaaacgc
     2281 gtattcatgt tgtgcagggt gatattacca aactggccgt tgatgtgatt gtgaatgcgg
     2341 ctaatccgtc attaatggga ggcggcggcg tcgatggggc cattcatcgc gcagcgggtc
     2401 cggccctgct ggatgcctgt ttaaaagtca ggcagcagca gggcgattgc cctacggggc
     2461 atgccgttat tacgcttgca ggcgatcttc ccgctaaagc cgtagtgcac accgtcgggc
     2521 cagtctggcg tggtggtgaa caaaacgaag accagctttt gcaggatgcc tatctcaata
     2581 gcctacgact ggtggcggca aacagctata cgtcagtggc ttttcctgca atcagtactg
     2641 gggtttatgg ttacccccgt gcggcagcgg ctgaaatcgc agtaaaaacc gtttcagaat
     2701 ttattacccg tcacgcttta cccgaacagg tatactttgt ctgttatgat gaagaaaacg
     2761 cccacctcta cgaaagactc cttacccaac aaggagatga atgatttgcc tcagctggcg
     2821 agcgcggtgc tgccactgtg ttcgcaacat cccggtcagt gtggactttt tcctctggag
     2881 aaaagtctgg atgcgtttgc cgcccggtat cgtctggctg aaatggcaga gcatacactg
     2941 gatgttcagt attacatctg gcaggacgat atgtcgggtc ggttactgtt ttccgccctg
     3001 ttagccgcag caaagcgtgg cgttcgcgta cgtttgttgc tggacgacaa caatacgccc
     3061 ggacttgata acattttacg cttgcttgac agccatccac gcattgaagt ccggcttttt
     3121 aatcctttct cgtttcgctt gctacgtccg cttggttata tcaccgactt ttcccgtctc
     3181 aatcgccgta tgcacaataa aagtttcact gtcgatggcg tggtgacgct ggtgggagga
     3241 cgaaatattg gtgatgccta ttttggtgca ggggaagagc cacttttttc ggatttagat
     3301 gtcatggcaa taggacccgt ggtagaggac gttgccgatg atttcgcccg ctattggtat
     3361 tgcaaatcgg tttccccctt acagcaggtg ctggatgtcc cggaaggtga aatggcggat
     3421 cgcatcgagt taccggcatc ctggcatgac gatgccatga cgcatcgtta tttacgcaaa
     3481 atggaatcca gtccgtttat aaatcatctg gttgatggaa cgttgtcgct tatctgggcg
     3541 aaaacacgtt tattaagtga tgatccggcg aaaggggagg gcaaggcaaa acggcattca
     3601 ctgttaccgc agcgcctgtt cgatatcatg ggctcaccca gtgaacgcat cgatattatc
     3661 tcttcctatt ttgtaccgac acgtgcaggt gtggcgcaac tcttacggat ggtgagaaaa
     3721 ggggtaaaga ttgcgatcct aaccaattct cttgccgcta acgatgttgc tgtcgtccat
     3781 gcaggatacg cgcgctggcg caaaaaattg ctccgctatg gggtggaatt atatgaactc
     3841 aagccgacgc gtgaacaaag tagtacgtta cacgatcgcg gcataaccgg taattcaggg
     3901 gccagcctgc atgctaaaac ctttagtatc gatggtaaaa cggtgtttat cggctctttc
     3961 aatttcgatc cgcgttcaac gttgctcaat actgaaatgg gcttcgtgat agagagcgaa
     4021 acgctggcac agttaattga taaacgcttt attcagagcc agtatgatgc ggcctggcag
     4081 ctccgtctgg acaggtgggg acggatcaac tgggttgatc gtcattcaaa gaaagagatt
     4141 attctcaaaa aagaacccgc caccagtttc tggaagcggg ttatggtcaa actggcgtcg
     4201 atattgcccg tggaatggtt attgtaaacg cggcttaacg ggctggtgct ttatcgttct
     4261 cacgcttaac aaccggttta ccagagaaca aaaacttcaa taatgggatg cgtaaatgaa
     4321 tttcatacag aattatcgca atccctacta cgaatatcag gccacagaga aaaccaagcc
     4381 agttggaggt gatgtgtggt gtaatgtatg cgccgaaaaa cagcgttaac gggtggtgaa
     4441 ccagatagat aaacagcgat gcgttaacaa aataagtcac ccgcgctgac tggaagttaa
     4501 gcaaacggtg gccaaaggag aagaccacat tcaccatcca cagaccgagg accatagtga
     4561 tcaccgactc ggtttcatac atccaggcat cgccactgcc atagcgctgg ttgagtaaat
     4621 aagcgacaaa cgccaatgct gctgcaaggg tacagccacg agacggcgtg gtaaacaagg
     4681 ctttaagatg agggaaaatg aaagccagtg cgccgaggat aaagaacggc aaataaaata
     4741 acgtttgcat gacaataaaa ttgaacatgc cgttactcag aatgggtgga tacacaataa
     4801 aaatcgttct tcttattacc gcataaccga tgccgaggca caaaaaaatc accgataatt
     4861 ttaccatcga gaatttttta ttcgttttat cagaattttc taaattattt ctgatgcgtt
     4921 taaatatcca tacgcacagc gtcgtcatga ccactaacac cagtaaaaac cacaggtgtg
     4981 atattaattc ccaggccaac gtattatatt tgtcatacaa tgacagccca ggccaacttt
     5041 ccgctttccc tttgacgtat tgcagcataa taaattgcgg caatgtcagt aaggggatgg
     5101 ctgttaacat cgggatccct acacgttcga cacgtacttt ccaccatttt ttcaagggat
     5161 agcgtaaaaa aagcatgtag gaaaagtacc cggatataac gaaaaacacc tgcatgcgga
     5221 acgagtggat gaagtcatta aaaagggtca gccacaatga cggttcggcg ctattcacat
     5281 gccatgtatg gctcgaatag attaaagaaa tatgaaaagg gatccctaac aacatcagcc
     5341 aggcgcggat ggagtcgagg aaatattcac gttgcgcggg tactggattc atatatggtt
     5401 aactaatctc ggatttttcg tcttatccct gtcgggttat gcctttaggc ttgttgccat
     5461 agcgacaccg acctgaccgc gccaggcgca ggcttcaagg tttttatgca tagcatcatc
     5521 gctaccacta accagaatgg aagcgtcagt aagacggttg ataaataaat ttgctggcaa
     5581 accctacacg aagtcgatgc ttctgtcttt aggagaagca cggaaagtga aaacggttgc
     5641 aatcaggtgc ttaatccatg agccagtgtg ctgaacgata ccgggattct gttgtcggaa
     5701 tggctggtta tccattaaaa tagatcggat cgatataagc acacaaaggg ggaagtgctt
     5761 actaattatg aaacataaac tacaaatgat gaaaatgcgt tggttgagtg ctgcagtaat
     5821 gttaaccctg tatacatctt caagctgggc tttcagtatt gatgatgtcg caaagcaagc
     5881 tcaatcctta gccgggaaag gctatgaggc gcccaaaagc aacttgccct ccgttttccg
     5941 cgatatgaaa tacgcggact atcagcagat ccagtttaat catgacaaag cgtactggaa
     6001 caatctgaag accccattca aactcgagtt ctaccatcag ggtatgtact tcgatacccc
     6061 ggtcaaaata aatgaagtga ctgccaccgc agtcaaacga atcaaataca gcccggatta
     6121 tttcactttc ggcgatgttc agcatgacaa agacacggta aaagaccttg gttttgccgg
     6181 tttcaaagtg ctttacccga tcaacagcaa agataaaaac gatgaaatcg tcagcatgct
     6241 cggggccagc tatttccgcg tgattggtgc aggtcaggtt tatggccttt ctgcacgcgg
     6301 cctggcaatt gataccgcct tgccatcggg tgaagaattt ccacgcttca aagagttctg
     6361 gatcgagcgt ccaaaaccga ctgataaacg tttaaccatt tatgcattgc ttgactcgcc
     6421 gcgcgcgaca ggtgcttaca aattcgtagt tatgccagga cgtgacacgg ttgtggatgt
     6481 gcagtcgaaa atctatctgc gcgataaagt cggcaaactg ggggttgcac cgttaaccag
     6541 tatgttcctg tttgggccga accaaccgtc gcctgcaaat aactatcgtc cggagttgca
     6601 cgactctaac ggtctgtcta tccatgctgg taatggcgaa tggatctggc gtccgttgaa
     6661 taacccgaaa catttagcgg tcagcagctt ctcgatggaa aacccgcaag gcttcggtct
     6721 attgcagcgt ggtcgtgatt tctcccgctt tgaagatctc gatgatcgtt acgatcttcg
     6781 tccaagcgca tgggtgactc cgaaagggga gtggggcaaa ggcagcgttg agctggtgga
     6841 aattccaacc aacgatgaaa ccaacgataa catcgtcgct tactggacgc cggatcagtt
     6901 gccggagccg ggtaaagaga tgaactttaa atacaccatc accttcagcc gtgatgaaga
     6961 caaactgcat gcgccagata acgcatgggt gcaacaaacg cgtcgttcaa cgggggatgt
     7021 gaagcagtcg aacctgattc gccagcctga cggtactatc gcctttgtgg tcgattttac
     7081 cggcgctgag atgaaaaaac tgccagagga taccccggtc acagcgcaaa ccagcattgg
     7141 tgataatggt gagatagttg aaagcacggt gcgctataac ccggttacca aaggttggcg
     7201 tctggtgatg cgtgtgaaag tgaaagatgc caagaaaacc actgaaatgc gtgctgcgct
     7261 ggtgaatgcc gatcagacgt tgagtgaaac ctggagctac cagttacctg ccaatgaata
     7321 agacaactga gtacattgac gcaatgccca tcgccgcaag cgagaaagcg gcattgccga
     7381 agactgatat ccgcgccgtt catcaggcgc tggatgccga acaccgcacc tgggcgcggg
     7441 aggatgactc cccgcaaggc tcggtaaagg cgcgtctgga acaagcctgg ccagattcac
     7501 ttgctgatgg acagttaatc aaagacgacg aagggcgcga tcagctaaag gcgatgccag
     7561 aagcaaaacg ctcctcgatg tttcccgacc cgtggcgtac caacccggta ggccgtttct
     7621 gggatcgcct gcgtggacgc gatgtgacgc cgcgctatct ggctcgtttg accaaagaag
     7681 agcaggagag cgagcaaaag tggcgtactg tcggtaccat ccgccgttac attctgttga
     7741 tcctgacgct cgcgcaaact gttgtcgcga cctggtatat gaagaccatt cttccttatc
     7801 aggggtgggc gctgattaat cctatggata tggttggtca ggatgtgtgg gtttccttta
     7861 tgcagcttct gccttatatg ctgcaaaccg gtatcctgat cctctttgcg gtactgttct
     7921 gttgggtgtc cgccggattc tggacggcgt tgatgggctt cctgcaactg cttattggtc
     7981 gcgataaata cagtatatct gcgtcaacag ttggcgatga accattaaac ccggagcatc
     8041 gcacggcgtt gatcatgcct atctgtaacg aagacgtgaa ccgtgttttt gctggcttgc
     8101 gtgcaacgtg ggaatcagta aaagccaccg ggaatgccaa acactttgat gtctacattc
     8161 tgagtgacag ttataacccg gatatctgcg tcgcagagca aaaagcctgg atggagctta
     8221 tcgctgaagt cggtggcgaa ggtcagattt tctatcgccg ccgccgccgt cgcgtgaagc
     8281 gtaaaagcgg taatatcgat gacttctgcc gtcgttgggg cagccagtac agctacatgg
     8341 tggtgctgga tgctgactcg gtaatgaccg gtgattgttt gtgcggcctg gtgcgcctga
     8401 tggaagccaa cccgaacgcc gggatcattc agtcgtcacc gaaagcgtcc ggcatggata
     8461 cgctgtatgc gcgctgccag cagttcgcga cccgcgtgta tgggccactg tttacagccg
     8521 gtttgcactt ctggcaactt ggcgagtcgc actactgggg gcataacgcg attatccgcg
     8581 tgaaaccgtt tatcgagcac tgtgcactgg ctccgctgcc gggcgaaggt tcttttgccg
     8641 gttcaatcct gtcacatgac ttcgtggaag cggcgttgat gcgccgtgca ggttgggggg
     8701 tctggattgc ttacgatctc ccgggttctt atgaagaatt gccgcctaac ttgcttgatg
     8761 agctaaaacg tgaccgccgc tggtgccacg gtaacctgat gaacttccgt ctgttcctgg
     8821 tgaagggtat gcacccggtt caccgtgcgg tgttcctgac gggcgtgatg tcttatctct
     8881 ccgctccgct gtggtttatg ttcctcgcgc tctctactgc attgcaggta gtacatgcgt
     8941 tgaccgaacc gcaatacttc ctgcaaccac ggcagttgtt cccggtatgg ccgcagtggc
     9001 gtcctgagct ggcgattgca ctttttgctt cgaccatggt gctgttgttc ctgccgaagc
     9061 tattgagcat tttgcttatc tggtgcaaag gaacgaaaga atacggcggc ttctggcgcg
     9121 ttacattatc gttgctgctg gaagtgctgt tttccgtgtt gctggctccg gtacgcatgt
     9181 tgttccatac agtcttcgtc gtcagcgcgt tccttggctg ggaagtggtg tggaattcac
     9241 cgcagcgtga tgatgactcc acttcctggg gcgaagcgtt caaacgccac ggctcacagc
     9301 tgctgttagg gttagtgtgg gcagttggta tggcgtggtt ggatctgcgt tttctgttct
     9361 ggctggcacc gattgtcttc tcgttgatcc tgtcaccgtt tgtttcggtg atttccagcc
     9421 gtgccaccgt tggtctgcga accaaacgct ggaaactgtt cctgatcccg gaagagtatt
     9481 caccgccgca ggtgctggtt gataccgatc ggttccttga gatgaatcgt caacgctccc
     9541 ttgatgatgg ttttatgcac gcggtgttta acccgtcatt taacgctctg gcaaccgcaa
     9601 tggcgaccgc gcgtcaccgc gccagtaagg tgctggaaat cgcccgtgac cgccacgttg
     9661 aacaggcgct gaacgagacg ccagagaagc tgaatcgcga tcgtcgcctg gtgctgctaa
     9721 gcgatccggt gacgatggcc cgtctgcatt tccgcgtctg gaattccccg gagagatatt
     9781 cttcatgggt gagttattac gaagggataa agctcaatcc actggcattg cgtaaaccgg
     9841 atgcggcttc gcaataaaaa cgtagttgcc tgatgcgcta cgcttatcag gcctacatcg
     9901 ttcctgcaat ttattgattt tgcaagattt tgtaggtcgg gtaaagcgtt caccccgcat
     9961 ccggcaaaaa caacgagccg ataaaaatac cggcgttatg ccggtatttt ttacggaaga
    10021 ggtatcaaat gcgtttaatt gtgatgagca taatggtgac cttgctgagt ggctgtggca
    10081 gcatcattag tcgcactata ccggggcagg ggcatggcaa ccaatattat cccggtgtgc
    10141 aatgggacgt gcgtgactcc gcctggcgtt acgtcacgat ccttgatctg ccattctccc
    10201 tggttttcga tactttactg ctgccgatcg acgtacatca tggcccgtat gagtgattaa
    10261 cgttcatccc actcatcagc tgcagtgcgt ccttcctcgg tatccagcgg tggctcaagc
    10321 tgaaattccc cctcgtccca ttcatgtaat gtattctcat cttgccactc ctggcggatc
    10381 tctatctcat catagtcgcc gtcaaagaca ctttgcgcag cttcaccgct aagcatgggt
    10441 aaacattcac cttcttcccc ttcgtcggca aaaaactcaa cttgccacat gatgtcgccg
    10501 tcctgcaaaa cgtatttttg ggcattgaac tgttgcacat tcgcgttttc ggcgtcgatg
    10561 ccggggttgt ctgcaagaaa ttcttcgcgg gcagcgtcaa tggcttcttc aagcgttgcg
    10621 tacatggtca tcacgattct ccctttgagt tgatgaggtt tcagggaaaa ggatagctga
    10681 ttctccgctt ttgcaagtat gaaaggcgaa aaatcagttc gatatctggg ggattcgacg
    10741 acgacgtaga ctgttccatg aatagactgc gttgaataac actacgccag cggtgacgag
    10801 aaataccgcc ctgaaaccgt agttcgctga aatcgctgct cccatcaatg gtccggtaac
    10861 gttgccaata tcacgaaacg attggttata gctgaagata cgcccggcga tctggttgct
    10921 cgagttgtaa accaacagtg tctgtacggc ggggagtaat gcaccatcgg cggcaccgag
    10981 caaaaaacgt aaaatcccaa gttgtaatgg cgtctgaacg taagacattg ggatcaacag
    11041 cagtacagaa aagatcagcg ccgtaatcag gatcttttcg gggccgattc gatcgccaag
    11101 tttgccgagt cgtggtgcac ttagcagagc cgccacgcct ggcaccgagg cgatcatgcc
    11161 actgataaaa gcgacgttac tgacgttacc cgccagttcg cggacataca gcgtcagaat
    11221 gggggcaatt gagcccgtcg ccacctggat gattaacgtg gtgacaaaca ggctgagtac
    11281 cagtttcggg tttttaagtg atgtcaccac ttcccgcata tgcagcatct cttttttgct
    11341 gaccggctgg aatttttctc tgatgcaaaa cagggtgacg aaaaagcaga gtatgagcac
    11401 actggcggta ataaagaata ccggacgtaa gccgtagcta tcggcgagca aaccgccagc
    11461 cattgggccg agcaacgcgc cgctaacgcc gcctgtggag agcgtaccca gcgcccagcc
    11521 gcttttatta cgcggtactt gtgtggcgat aagagcatta gcgttgggga caaatccgcc
    11581 aagtaaacca agaagcgccc gcaggatcaa aaactgccag atattttgtg ccagtcccat
    11641 caacaccatc acgatgccca tgccgagagc agagcgtaat agcatgagtt ttcggccttt
    11701 acggtcggcg agtccacccc aaaacggtga ggcgatggcc gaaaataaaa atgtaatgct
    11761 gaagacaata ccggaccaca tattcagggc ggagtggccg gtaacgccaa gctgctcaac
    11821 gtagaggggt aagaagggca ttaccaggct gaaggcggca ccggtaagaa aacagcctag
    11881 ccaggcgacg atcaggtttc gtttccagtt tataggggtg tcattttcac agggtgacat
    11941 agcaatccgc tgttggtgcg ccaggcgcgg tgaacataag aagaaaagat aagcacacta
    12001 attatgcgcc cgacttccag ggggcgcaat ccagagagct tttatcgcta aatcaggggg
    12061 attttctgtg gtaatgccgg atgccattct gaagcatcca gcatggaaga tttaatagcg
    12121 tgaaggaacg ccttccgggc gtgttttaaa gcgacggtgt aaccacatat actgctctgg
    12181 tgccatcatg atgcattttt cgaccacttt gttcatccac gcggcggtag tttcggcatc
    12241 atccagcggc ggagaacact ctggcggcag tataatcaac tgataccctt tgccatctgg
    12301 cttacggcgt ggaacgaagg gcaccagaca tgcgccggac atccgtgcca gcatccaggt
    12361 tccggtcgtg gtcgcagcct gctcaacggc aaacaacggg acgaaaacgc ttgagcgcgg
    12421 gccgtaatca tgatccggtg cgtaccagac cacttcgcct tttttcaggg ctttaatcat
    12481 gccttttaaa tctttgcggt cgagcatcga tttatttgag cgcaaacggc cccaggtttg
    12541 tagccagtca atcagtggat tatcgttcgg gcggtaaacg ccaatccccg gttcctgcat
    12601 accaaactgc cgcgcaccca gctccagtgt cagaaaatgg atgccaacta acaggatgcc
    12661 gcgtttttgc gcctgcacgt cacgaatgtg ttccatgccg atcacttccg tccagcgggc
    12721 gattcggcgg tccggccaga accacgccat gccggtttcc atcaggccca tgccaacgga
    12781 ttcgaaattc ttcaccacca ttttacggcg ttcttgttcg ctcatttccg ggaagcacag
    12841 ttccaggttg cgatgcacaa tttttgcgcg tcgtttcata aaacgtaacg ccagttttcc
    12901 taatccacaa ccgaggcggt agataaccgg gtagggcaat tgcacgacca accaaagtac
    12961 gccaataccc aaccaggtta accaataacg cggatgaagc agtgcggtgg agaacttggg
    13021 tagattcgtc atatcaatcc tgtttttcaa cctatacggg caattgtatg tattgtcgca
    13081 ttttttcgtc cgcaaccaaa atttgtgact gaagactggg cgaaattgcc gcgcttgtaa
    13141 ataacaaata atttttaatg ctaaaatgta gcgtaaaatg tgtggatgtt aattatcgat
    13201 aattgctata tcatgccgcg gatttttact ttcccatctc gcaggaaccg tacaccatgc
    13261 cagtgttaca caaccgcatt tccaacgacg cgttaaaagc caaaatgttg gctgagagcg
    13321 aaccgcgaac caccatttcg ttttataagt atttccacat tgccgatcct aaggtgaccc
    13381 gtgacgcttt atatcaactg tttaccgcgc tgaatgtttt tgggcgagtg tatctggcgc
    13441 atgagggcat taacgcgcaa atcagcgtac ctgcgagcaa tgttgaaaca tttcgcgcgc
    13501 agctctatgc cttcgacccg gctttagagg gtttacgcct gaatatcgcg ttggatgatg
    13561 acgggaaatc cttctgggta ctgcgtatga aggtacgcga tcgcattgtt gccgacggta
    13621 ttgacgatcc tctctttgat gccagcaatg ttggtgagta tctgcaagcg gcggaagtga
    13681 acgccatgct tgacgatccc gatgcattgt ttatcgacat gcgtaaccac tatgagtatg
    13741 aagtggggca ctttgaaaac gcgctggaaa taccggcaga taccttccgt gagcagctgc
    13801 caaaagcagt tgagatgatg caggcacata aagataaaaa aatcgtcatg tactgcaccg
    13861 gcggcattcg ttgtgaaaag gccagtgcct ggatgaaaca taacggattc aacaaagtct
    13921 ggcatatcga gggcggaatt attgaatacg cccgtaaggc gcgcgagcag ggcttgccgg
    13981 tgcgttttat tggcaaaaat tttgtttttg acgagcggat gggcgaacgt atatctgatg
    14041 agattatcgc gcactgccac cagtgcggtg cgccgtgcga cagccatacc aactgtaaaa
    14101 atgatggctg ccatctgttg tttattcagt gtccagtatg tgcggaaaaa tacaaaggtt
    14161 gttgtagtga gatttgctgc gaagaaagcg cgttaccgcc agaggaacag cgacgccgtc
    14221 gggcaggacg tgaaaatggc aataagatct ttaataagtc tcgtggacgt ctgaatacaa
    14281 cactgggcat tcctgaacca acagaataaa tatcattgcc ggatgcgtgc catccggcaa
    14341 catttcactc ttacttctgc tgtacgcctt ccactgaaat aatcagatcc acttcctgag
    14401 acgctggacc taaatctgtc ttgatattga agtctttgag ctcgattttg ccttcggcct
    14461 caaagcctgc acgtttaccg ccccacgggt cgtcaccctg accaattaat ttcgcttcca
    14521 gtgtgacggg cttggttaca ccattcagcg tcagatcgcc ggtaatatcc agttcgtcac
    14581 cgtctttctt cacgctggtg gaggtgaatg ttgcctgtgg atattttgcg gtattgagga
    14641 aatccgcact gcgaagatgt ttatcgcgtt cggcgtgatt agtatcgacg ctggtggtgt
    14701 taattgtcac attcacttta tcggcagctg gatttttttc atcaaaggta aaagtaccgt
    14761 cgaaatcttt gaaggtgcca tataaccagc tatagccaag atgctggatg cggaagttaa
    14821 caaaggcgtg ctgaccttct ttgtcaattt tgtaatcggc ggcaaccgct gaaccggcag
    14881 agaacatcaa ggaagcgaag gttaaaccaa gcaggctttt tttcattttt tatactccat
    14941 agtcagatga cgactttccc agcatgcgct tcagagtgtc gtctttatcg atgaaatgat
    15001 gcttcagggc cataaatccg tgcataacgg acagtactac gacgctccac gcgagccaaa
    15061 aatgcagggc accggcaaag tcagcctgtg cgccagcgtc ggcgagggtc gcggggacgt
    15121 caaaccagcc aaaaacgctg atcggtttac catcggcagt tgagatcaga tagccgctga
    15181 tgccgatggc gaacagcaat aggtacaatg cgagatgtcc agcttttgct gcaagacgag
    15241 tcatgggcga ataactcggc agcggacccg gcggtggaga tatgacacgc cacagaacgc
    15301 gaataaccag ccccatcatt aacaaaatac cgatgctttt atgcagttcg ggtgctttgt
    15361 gataccagcc atcgtaataa ctgagcgtga ccatccacag acctaaagca aacatgccgt
    15421 agacaataat tgcgcttatc caatgtaagg ccgcagatat aacaccatag cgttcagggg
    15481 tatttgtgaa tgacataagt acatccaaaa cgaatttgcg agagaaagaa aatggcgtgt
    15541 cggagaatga attgcaacac aaaaataaca aattgaatga tatttatttt tatttcaata
    15601 attttgaatt aataatgaaa gaatcttcat aaatttagtg ggttaagcat ctggaaggtc
    15661 tagtgagtgc tgtagaggca ggaagaatct cattcaattt ttcacgctaa atgaaaggga
    15721 gattaaatat ttgtcagttt atgtcagtgc ggaaaactaa aaataaaaca catagatcag
    15781 atccataatt gccaacaatc cccagagaaa aagataaagc atcagatgct cgcgaatatt
    15841 attgatgaga tagtgcaaca gtcgacgcat taaatactcc tgtaaaaacg gcctcattct
    15901 ggaggccgta atgatgatta ttggaagcgg gaaagcctga atggcgtcaa atcaaaatcg
    15961 ctttttttgt cttgcgcaaa atcggcagct atttccccta aaactgacgc aaatttaaaa
    16021 ccgtgcccac tcaggccggt aatgagcagt gtattatcgt ggccgggtag ggtatcgata
    16081 ataaaatctt cgtcaggcga attatcatag gtgcaggcag cgccgtacag gcagcaaccg
    16141 atacccggca acacattgcg caagaacggg aaggcttccg aaccatcgct gaccacttcc
    16201 gcaaacggaa cacgttcatc cgctgaatgg ataacctggc ctccgttatg tttgccaatc
    16261 ttcaacgcgt cgttttctgc cggaaaacca taatattgat cgccattggg cagttcaccg
    16321 gtaaacgccg ggaatttatt cttcacgcta tagcgaccat cggcctgata ccaggcaaat
    16381 actttgcgta cgggctggac aggcagctcc gggagtaggt cttttaccca tgttcccgcg
    16441 cagacaatcg cttttttcgc ctgatacaca ccgtcagccg tttcaatagt tacgccatca
    16501 tcgtcatgac gaattgcggt gaccgggcag ttgaacagct gcgcacagcc cgcttccttc
    16561 gccagttgga tccaggtttt aatcgccagt tcgctgcgca aaaaaccaga atcagtttca
    16621 aataaaccga tgtagttgtc cgggacgcgt atttctggcc agcgggccat aatcccttgc
    16681 gcgtcgagct tttcaacgtt gagttgccac tgttcggcgc tgtgggcgac gttggcgaga
    16741 aatgcggagt cagccggacc gaggttaatg acaccagagc gtacaaaaat gggatcatct
    16801 tcgttgtggc gggaaagttc atcccacagc atttgcgcgc ggaggaccag cgggacatac
    16861 ttttcacctt caccataagc atggcgaatt aatcgcgtat cgccgtggtg gctgccgtgt
    16921 tgatgcggtg gcatatgggc gtcggtcatt agcacgttta aaccggcgcg ggttgcataa
    16981 tacccggcgg cagcgcctac ggaaccgctg ccaataatga tgagatcgta tttcattttt
    17041 ttctctctgc tctcatggtt taagcagagt aattaactga atgcagatat acaacccaga
    17101 aatggtaaag gcaccggtga ggtgcctttt gggtggatgg tcatgtcatg ttaatgacgc
    17161 cgatactcgt ttacctggaa atcaccggat tcaattttgg cgattcctgc ttctaatatc
    17221 gaaataaatt gtctggcaac atcagtggtt aaccagagcg tctgaccaac ttcagtccct
    17281 tcctgctcgg acttatttgg ggtctggtag tgcaaacgca acatcagcgc atcatagcta
    17341 tcaacggtgc tgatgtccca ccctacgagc ggatgagtct gaatgacttc attatttttt
    17401 tccatcatgg ccccctaatt cgtgttacta gacaacgtta ttcgaggttc aatgcgtttt
    17461 tatctgaagc aacttcagta taccaataag taagggtatt caccgaattt ttaaaggggg
    17521 gaacagataa atttctgctt ttaagattta tatgaacaat aaaacagcat gtcattcata
    17581 tttttttagc atattgtgca attattttga ggaagtgtag aaattttgta ctcacaattc
    17641 gcaagtaaaa taaaaaagcc ggggcgaccc ggcaaaaaca aaaatcactg catattattc
    17701 gctgacaaac cagtcatcgg cgctttccca cgtttcctgg agaatttcgc taatgcgctg
    17761 tttatcttct tttgtcgcgc caataaccga taaattattt gctgcggcat aacgtaccga
    17821 tacgtggcct tcattatcag gaaacgcata ctgaatacgg cgggaaagtt cgccagccag
    17881 ggcgtcaata gccccagctg gcaatggaga agttttcgct atggtgactt caattcgcat
    17941 aatagccccc tgttgaatac actggttatt tatacaggta aaataaccta atgacaacag
    18001 gaagctacga tttttattgt ttaacggacc agcgtaccgt ttctccggcg aggaatggca
    18061 ccagcgtgtc atcagtcagt gcgatgcttt cagcaacctg ttgctcttca cgtaccagtt
    18121 cgatgaatgt gtcgttgacc ggcaagccat agaactgcgg gccgtttaca gaacagaatg
    18181 cttcaaagtg ctgcaaagca ttcatctctt caaagacggt agcgtaactg cccagcgcgg
    18241 ttggggcgtt gaaacagccc gcgcagccgc agctgctctc tttgcgatga cgtgcatgtg
    18301 gcgcagaatc cgttccgagg aatacacgat taaaaccgct ggcgaccagt tcacgcaatg
    18361 cctgttggtg gatattgcgt ttgaggatgg gtagacaata caggtgcgga cgcacgccac
    18421 caaccagcat atggttgcgg ttaaacatca gatgctgcgg agtgatggtg gcagccagcc
    18481 gttcatttcc gtcacggaca tagtcggcag cgtctttggt ggtgatatgc tcaaaaacga
    18541 ctttcagcgc ggtcaggcgc tggcgcagcg gttccatcac gctttctata aagcgcgctt
    18601 cacgatcaaa aatgtcgata tctgcatgtg ttacttcacc atgcaccagt agcggcatac
    18661 cgattttttc catgcgctca agtaccggca tgattgcgtc aactgacgta acgccgtggc
    18721 tggagttagt ggttgcgttt gccggataaa gttttgcagc ggtgaacacg ccttcgttaa
    18781 atccgcgatc cagctcatta ggatccagcg aatctgttaa ataacaggtc atcagcgggg
    18841 tgaaatcgtg cccggcaggt acggcgtcaa gaatgcgctg gcgatacgcc acggcagcct
    18901 caacggtggt cacgggcgga gcctgattgg gcattacgat agcccgtcca taaatttcgc
    18961 tggtatacgg cacgacagtt tttaacatgt cgccatcgcg gaggtgaagg tgccagtcgt
    19021 ctgggcggcg gatctttaat acctgggatg gtgcagtcat taatctctat gctccggctg
    19081 aagggatgtt tttgccggac acaaaggata agcggaaacg ttttcctttg cacgaaaaat
    19141 aaagggcgcg aatgcgccct cgtgattaat cagtaaacgg aatgacaatt tcgcctggct
    19201 tcacttcaat gcctttcgcc agtttcttcg ccattgcttc gccattgctg ccatcttcgc
    19261 gcaggacgta agcaggttgc tggttaaagt aattgcgtaa tgcctggttt aaatagggaa
    19321 gcaacgtttg catcaccgtt tgcatttttt ccggttgtac cgtcgcatcg accacttcca
    19381 tctctttcag gaagatcgcg cctttttctt tatcaaacac gggcagcgct ttcagcttca
    19441 gtttcatggt cgctttctgg ctaccgaaca gggagttcat gtccagattg gcgtctccgg
    19501 ttagggtaac cttattcggc tcttcgcgac caatttggct ggtcaggttt gtcagaacaa
    19561 tatgggcgtc agccacgccg ggtaaaccga tatcttttga gaaattatta tgtttcgcaa
    19621 gcgactggtt aatttcttgt tcggtgatgg tgtattgggt gagttgatta cagccaacga
    19681 gcaggccact gacgatcaat gcagcggcaa ataaaaactt gttcatggta gtcctcgaca
    19741 tgaaatctgc gtcaatatcc tgacacaacg cagcatgtgt caccagcgat aaacgcgcca
    19801 gcagaaaaaa ctgaaaacgg cggcaacccg cgaatacagg ctgccgcggc gggtcaggat
    19861 taaatcgcca ttgatgataa caaattgatt tgtgtctgtt tcgccatatt atcgcggtaa
    19921 tcagcaacgc ggcttggcca gttgattccg gctactagcg tcagattacg caggagcgga
    19981 aatagctgaa tatcatcttc cgaaagttcg ccattcacgg cgttcggttt gacgatcagt
    20041 ttgtccagcg cacgtaaatc atcgctgata ttcttaatca gaccgtcaga gtgggccagc
    20101 aggtcggcaa aattacccgc gctggcctct ttcttgtcga cgaaatattt gcgcgcggca
    20161 ggagtagaaa actcatcaaa tgccgatttg gcaaaacgcg gcaacagcag tttgttggcg
    20221 tagccattga ccttgcgcag ccactcttca atggcagggg aacgtttgcc ggtcagtaac
    20281 ggtttgccgt cgagtttatc gacatagtga acgatgtcca tgctttctgg catatagcgg
    20341 ctgtcatctt tttgcagaat gggaacctgt ttttgaccga ccatccgggt gggtgtttct
    20401 gcgtcgtcgt tgagcagaac atgtaattcg acggggatat ttttcaggcc gaaaatcatg
    20461 cgggctttga ggcagtaagg gcagtgatcg taaatgtata gcttcacgtg actcctccat
    20521 ttggctgtca gttcactttc agtatggagg agtcaggggg gagtatcaaa tcaggcgtcg
    20581 cgttcaagca aacgacgcgc ggcgcgtttc tggctaaact gccaacccag cgcaaggaaa
    20641 gtgaagatgc caataatgcc cagcatcatc cacggaagct ctggctggtg cgccgatttg
    20701 cccaggtcaa acagccagcc gccaccgata taaccaatag cgccgccaat cgccagaccc
    20761 agacggctaa accccatata gctgccgcga gctcttgcgt ctgccagcga agcacttaag
    20821 gtttcacgcg caggctcggc aatgatcgac ccgatataaa acagacaaat cagggtgaaa
    20881 agttgttgca ggccgctgac catgcctact ggcatcatgc ttaatgacat tatcaacagc
    20941 ccagccatta accggtgttc cagacgaaaa tgcttttcac tccagcgggc gatagggtag
    21001 agcaacgtta acgacagaca cgcttcaatg gcatacatcc atttaacggc agagggcgcg
    21061 ccagccacgt cgttgaccat aattggcagc atcagcatca cttgtacagc cagcatgtag
    21121 taacccgcca gcgtcaggac ataggtgaca aaacgcttgt cgcgcatcac gcgggtcatg
    21181 ccttcgcgaa cgggcgtgcg cacggtggag agtttccatg ccggtaacaa ccacgcattg
    21241 aacgctgcac acagcacaaa caaaactgcc cctgtggcgc agaccaggcg aaagtcgtat
    21301 tgcaacagcc agctccccaa caatgcgcca atgaccgcac cggcactgtc ctgcatcatc
    21361 aacagcgaga aaaaacgacc acgctgctgt ggacggatta atttcaccac cagcgccgaa
    21421 cgcggaggat caaacaacgt gccgccgagt cccgaaagca ggcatgaaaa ccacagtagc
    21481 cacggttcgt gggcgatacc cattgtggcg aatccggcgg cacgcatcag cataccggtg
    21541 acaatcatcg gtttggcacc aaagcggtcg gcaattgcgc cgccgaaaat acccagacct
    21601 tgctgaataa actggcgtag accgagggca ataccgacca tgacggcggc ccagcccatt
    21661 tgatcaacga agcggataga gatcagcggg aagacaacaa agaaccccag cacgaccagc
    21721 atattatcga tgagcaggaa atatttaccc aggttcctcg cctgcgacac gcgggacatt
    21781 tcccctcccg ggaaataaaa gatgagcact ttctattctg cattgccgct aagacttttc
    21841 ccatcacttc ggggacaata tttttttatc aaaacgtcgc tttgatagag agtttttatc
    21901 aaatatgtga ataatgcaga aaatggcatt ctggactttt cacagggtct ggttgcgcag
    21961 gtatagtaat acttacagcg tattaaagac gttacgggaa ggagtaggta tagaatgttt
    22021 ggctatcgca gtaacgtgcc aaaagtgcgc ttaaccacag accgactggt cgtgcgtctg
    22081 gtgtatgatc gtgatgcctg gcgtctggcg gattattacg cagagaatcg ccatttcctc
    22141 aagccctggg agccagtgcg cgacgaaagc cactgttatc catcaggctg gcaggccagg
    22201 ctagggatga ttaacgaatt tcataaacaa ggttcagctt tttactttgg cttattcgac
    22261 ccggacgaaa aagagattat tggcgttgcc aatttttcca atgttgttcg cggctctttt
    22321 catgcctgct atctcggtta ttcgattggg caaaaatggc agggcaaagg actcatgttt
    22381 gaagccctga ccgcagccat tcgttatatg cagcgcaccc aacatattca tcgcattatg
    22441 gctaattata tgccgcacaa taaacgcagc ggtgatttac tggcgcgact gggttttgaa
    22501 aaagaaggct atgcgaaaga ctatctgttg attgatggac aatggcgcga tcacgtactg
    22561 acggcattaa ctaccccaga ctggacgccc ggccgctaag gagaatctca tgaaatatca
    22621 acttactgtc atggaagctc gcgtgattgg ttgcttgctg gaaaaacagg tgaccacgcc
    22681 ggagcaatac ccgctctccg taaatggtgt agtcacggcc tgtaatcaga aaacgaaccg
    22741 tgaaccggtt atgaatctga gtgaatctga agtgcaggaa cagctggata atctggtcaa
    22801 acgtcattat ctacgcacgg tgagcggttt tggtaatcgg gtcaccaaat atgagcaacg
    22861 tttttgtaat tcagaatttg gcgatctgaa actgagcgcc gcagaagtgg cgttaatcac
    22921 cacgttgtta ttgcgtggtg cccagacgcc gggggaactg cgcagccgcg ccgcgcgaat
    22981 gtatgaattc agcgatatgg cggaagtgga gtcgacgctg gaacaactgg cgaatcgcga
    23041 agatggtcct tttgtggtgc gtctggcccg cgaaccgggt aaacgcgaaa gccgctacat
    23101 gcatcttttc agtggtgagg ttgagaatcc gccggcggta acggatatgt cgaacgcagc
    23161 tgacggtgat ttacaggccc gcgtcgaagc cctggaaatc gaagtggcag aactgaaaca
    23221 gcgtcttgat tcgttgctgg cccatctggg agattaaagt gaaaaaatta cgtatcggcg
    23281 tagtggggtt aggcggcatc gcgcaaaaag cgtggttacc ggtgctggcg gcagcgtctg
    23341 actggacgtt acaaggtgcg tggtcgccta cgcgcgccaa agccttgcca atttgtgaca
    23401 gctggcgcat tccttatgct gattcgttat ccagccttgc cgccagttgc gatgcggttt
    23461 ttgtgcattc cagcaccgcc agccactttg acgtggtcag tacgttactc aatgcggggg
    23521 tacatgtctg tgtcgataaa ccgctggcag aaaatctgcg cgatgctgaa cggctggtgg
    23581 aactggcggc gcggaaaaaa ctgacgttga tggtcggttt taaccgtcgt ttcgcaccac
    23641 tctacggtga gttaaaaacg caactcgcca ccgcgtcctc gctaagaatg gataaacatc
    23701 gtaccaatag cgtcgggcca cacgatcttt atttcacatt gctggatgat tatttgcatg
    23761 tggttgatac cgcgctgtgg ttgtctggcg gcaatgcaac tctcgaaagc ggtacgttgc
    23821 tgaccaatga gtccggcgaa atgctgtttg ccgagcacca tttttcggct ggtcctttgc
    23881 agattaccac ctgtatgcat cgccgtgccg gaagtcagcg tgaaaccgtg caggccgtga
    23941 ctgatggtgc gctcatcgac attacggata tgcgcgaatg gcgtgaggag cgcgggcagg
    24001 gcgtagtgca taaaccgatt cctggttggc agagtacgct cgagcaacgt ggatttgtcg
    24061 gctgtgcgcg gcacttcatt gaatgcgtgc aaaatcagac agttccgcaa accgccggcg
    24121 aacaggccgt gctggcgcaa cgtatcgttg acaagatctg gcgcgatgcg atgagtgaat
    24181 aaaccctgta acatctggcg gtagcaattc accataatcc aggtagacta ttcgcctctt
    24241 tcagcgcctg ccttgcaggc gttttgcccg tgggtctgga ttagaacacc gatgaattta
    24301 ttaaaatcgc tggccgccgt cagctcgatg accatgtttt cgcgtgtgct tggcttcgca
    24361 cgagacgcaa ttgtcgccag aatctttggc gcagggatgg caaccgacgc ctttttcgtc
    24421 gcttttaaac ttcctaactt gttacgccgt atctttgccg aaggggcatt ttcccaggca
    24481 tttgtaccga ttctggcaga atataaaagc aagcagggtg aagacgccac gcgggtcttt
    24541 gtctcttatg tttctggcct gctgacactt gcgctggcgg ttgtgacggt cgctggcatg
    24601 ctcgctgcac cgtgggtgat catggtgacc gcgcctggct tcgctgacac tgccgacaaa
    24661 tttgccctga ccagccagct actaaagata acctttccct atatcttgct gatctccctg
    24721 gcgtcgctgg tgggagcgat tctgaatacc tggaaccgct tctcaattcc ggcgtttgcc
    24781 ccgacattgc tcaatatcag catgattggt tttgcgctgt ttgccgcgcc gtactttaat
    24841 ccaccggtgc tggcgctggc ctgggctgtt acggtcggcg gcatcctgca gctggtgtat
    24901 cagctacctc atctgaagaa gatcggcatg ctggttttgc cacgcattaa cttccacgat
    24961 gccggagcaa tgcgtgtggt gaaacagatg ggaccggcaa tccttggcgt ctctgtgagc
    25021 cagatctcct taatcatcaa caccattttt gcctcgtttc ttgcttccgg ttcggtgtcg
    25081 tggatgtatt acgccgaccg cttaatggag tttccgtccg gtgtgctggg cgtggcgctt
    25141 ggcaccattt tgctaccgtc gctgtcgaaa agttttgcca gtggcaatca tgatgaatac
    25201 aaccgtttga tggactgggg gctgcgtctt tgtttcctgt tggcgctgcc gagtgcggtt
    25261 gcgttgggca ttctttccgg tccgttaacc gtttcgctgt tccagtacgg taaatttacc
    25321 gcctttgatg cgctgatgac ccagcgggcg ttaattgcct actcggtggg tttgattggc
    25381 ctgattgtag tgaaagtgtt ggctcctggc ttttattccc gccaggacat taaaacgcca
    25441 gtgaaaattg ccatcgttac gctgatttta acgcaattga tgaacctggc gtttattggt
    25501 ccgttgaaac atgccgggct gtcactttct attggtctgg cggcgtgtct gaatgcttcg
    25561 ctgctttact ggcagttgcg taagcagaaa atctttaccc cgcaacccgg ctggatggcg
    25621 tttctgttgc gtctggtggt ggcggtactg gtgatgtctg gcgtgctttt aggtatgtta
    25681 catatcatgc cggagtggtc attgggtacc atgccctggc gtttactgcg tttaatggcg
    25741 gtcgtgctgg cggggattgc cgcgtatttc gctgcactgg cggtactggg cttcaaagtt
    25801 aaagaatttg cccgccggac ggtgtaacga tacattccgg cccgcagtgc aggccggagt
    25861 taatcttcag atcgaaatct ttttactgcc gcgatgggtt gttgaggtct gaccgttcgc
    25921 cccatacagc gtcggttcct gatgcggttt caacatttcc agcgcctgtt gattgcgttc
    25981 aatctgtcct tccagtaacc agccgttatg ttgattcagt tggcgtagtt gctgcgtttt
    26041 gccagtaatt tcctgccagc gttgactaat atcaacgcta tttgctgtat tgggttcttt
    26101 cctgcgtaac tgttcgaggt aatccagcgt cgccagcagt gagctttttt gttctgtaat
    26161 ccattgcaac tggctgccgt tgatctgccc catagagaga tgttgctgct cttgatccat
    26221 taccgttttg agatcgttaa gcacagcgga catctggtcg aggatctctg caagacgcgt
    26281 catacgatca attactctgc aagtcttgct gcgcttcgtt gatcagcgca tcggcaattt
    26341 tgccggtgtc catttttagt tcaccgttac gaatcgccag ctttaacgct tcgacacgtt
    26401 caagattgat atcactgctg ccgggttgca tcagttttgc ttgcgcgtcg cttaacgtca
    26461 cactggtgct ggtggaggcg gttgtttttg ccgcccgggt gtttgttacc ggcgcgtcag
    26521 tggtttcgcg cggttgaacg gtgcttactg gcttcagagg cgaagtgcga tcaatactca
    26581 tggtttattc ctcattgagg gcgcttttat catgtgttgc ttatttatcg gcaagggacg
    26641 ggtaatcttt aacagcttac aggtttataa gaatattccc atctgcatca acaacgccgc
    26701 tgaccacttg tcccgatatc atgcgcaccc gcgcattctg tgcgacggct gcattgttca
    26761 gcgcctgacc ttctgcgttg gcgctaaacc catcaccgct ggcgatcacg ttgacacgtt
    26821 gtcccgcttt tacccgccat gcctggcgaa agtgggttaa ctggataggt tgatcgggtg
    26881 atagatcgcg caggctaacg gcatcaacaa gttgattgat atccagcacc gtacgcggtg
    26941 gcaaggtatc cagccgtccg cgtttcagtt tgacattgct agcttccagc tttcctccac
    27001 gcacaatggg catcgcggca accacataat ttcctgtggc ctgcacatta acctgtaaat
    27061 atcgtttgtc gttaccgcag cgtgccaaca cattcacatt gccccacaga cgggaattat
    27121 tgctcatcga aagcaatggc tgatcgcacg gcggtagcag attgggtgct gtacgaatag
    27181 aaacacgaac ctcatcactt acccccgcga gttgggcgct aaaaaagttg tgcaattgcg
    27241 atgtgagatt gctcgccgta cttaacggac tgaacagtat cgcgatgatc gccacgctac
    27301 gttttattgt cagcattttc gtccccagcc atttctacaa cgtgaattgt acctgtccgc
    27361 aatgaccatc aacggcataa atagcgaccc attttgcgtt tattccgccg ataacgcgcg
    27421 cgtaaaggca tttaagctga tggccgaatt ttgatacctg cggaggagat atgctcgata
    27481 agctcgacgc cgccttacgt tttcaacaag aggcgctcaa tctgcgcgcc cagcgtcagg
    27541 aagtgctggc agcaaacatc gccaatgccg atacccctgg ttatcaggcg cgcgatatcg
    27601 attttgccag tgaacttaaa aaagtcatgc aacgtggacg ggatgcaacc agtgtggttg
    27661 ctctgacgat gacctcaacg aaacacattc cggcgcaggc gctgacgcct cctaccgcag
    27721 aactgcaata ccgtattccg gaccagcctt cgcttgacgg taataccgtc gatatggatc
    27781 gcgaacgcac ccagtttgcc gataacagcc tgcaatacca gatgagcctt agcgtgttga
    27841 gcgggcaaat caaaggcatg atgaacgttt tacagagcgg aaattaacgg atggcactgc
    27901 tgaatatttt tgatatcgcc gggtcggcgt taactgccca gtcccagcgc ctgaacgtgg
    27961 cggccagtaa tctggcgaat gctgatagcg tgaccggtcc cgatggacag ccatatcggg
    28021 caaaacaggt ggtattccag gttaacgctg caccaggtgc tgcgacaggc ggcgtaaagg
    28081 ttgccgatgt tatagaaagt caggccccgg acaaactggt ttatgaaccg ggtaatccgc
    28141 tggcggatgc aaagggctac gtgaaaatgc caaacgttga tgtcgtcgga gagatggtta
    28201 acaccatgtc ggcgtcacgc agttatcagg ccaatgttga agtgctcaac acggtgaaaa
    28261 gcatgatgct gaaaaccctt acgctcggtc aataaaggag aaagctatgt ctattgcggt
    28321 aaccaccacc gatccgacga ataccggcgt cagtaccacc agcagtagtt cgctcacggg
    28381 cagcaacgcc gcagatttac aaagcagttt tctgactttg ttggtggcgc agctgaaaaa
    28441 ccaggacccg accaatccaa tggaaaacaa cgagctgacg tcgcaattgg cacaaatcag
    28501 cacggtcagt gggattgaaa aactcaatac cacgctcgga tctatttccg gacagattga
    28561 taacagccag tcgttacagg ccagtaacct tatcggtcat ggcgtgatga tccccggtac
    28621 cactgttctt gcgggaaccg gcagtgaaga aggggctgtg accacgacca cgccgtttgg
    28681 tgttgagttg caacaggcag cagacaaagt gacggccacc atcaccgata aaaacggcgc
    28741 ggtcgtccgt accattgata tcggtgaact gactgccgga gtccatagtt tcacctggga
    28801 cggtacgttg actgacggca gcactgcgct gaacggttct tacaatgtag cgattagcgc
    28861 cagtaacggt ggtacacaac tggttgccca accgctgcag tttgctctgg tgcagggggt
    28921 gatccgcggc aacaacggta atacgctgga tctcggcact tacggcacca ctaccctcga
    28981 cgaagtacgg cagataattt aagccttcac atttcaggag tcagtcatgg ccttttctca
    29041 agcggttagc ggattaaacg ctgccgccac caacctcgat gttattggca acaatatcgc
    29101 caactccgcc acctacggct ttaaatcagg cacggcctct tttgccgata tgtttgccgg
    29161 ttcgaaagtg ggactggggg taaaagttgc tggtatcact caggacttta ccgatggcac
    29221 gaccaccaac accgggcgtg gtctggacgt tgctattagc cagaacggtt ttttccgtct
    29281 ggtagacagc aatggttcgg tgttctacag ccgtaacgga caatttaagc tggatgaaaa
    29341 ccgtaacctg gtgaatatgc aaggtttaca gctgacgggg tacccggcaa ccggtacgcc
    29401 gccgactatt cagcaagggg cgaatccgac caatatttcg attccgaata ccctgatggc
    29461 agcgaaaact accaccacgg cgtcgatgca gatcaacctg aattccagtg attcgcttcc
    29521 ctctgttaac gcatttgatg ccagcaatgc ggatagctat aacaaaaaag gttcggtgac
    29581 tgttttcgat agtcagggta atgctcatga tatgagcgtc tactttgtga agaccgggga
    29641 taataactgg caggtctaca ccctggatag cagtgatcca acaggcacag ctaaccctgc
    29701 aacgacgctg gtgtttaatg ccaatggcgt tctgacctca aatccaacag cgaatatttc
    29761 cactggcgca attaacggcg cagatcccgc cacgtttagc ctgagcttcc tcaactccat
    29821 gcagcaaaat accggcgcta acaacattgt ggcaaccacc cagaacggct acaaaccggg
    29881 cgatctggtg agttatcaaa tcaatgatga cggtacggtt gtcggcaact attccaacga
    29941 acaaacccaa ctgctggggc agattgtact ggcgaacttt gccaacaacg aaggtctggc
    30001 atccgaaggc gacaacgtct ggtccgctac gcaatcttct ggcgtggcgc tgttagggac
    30061 agccgggacg ggcaactttg gcacactgac caacggtgcg ctggaagcgt ccaacgtcga
    30121 tctcagtaaa gaactggtca atatgatcgt tgcccagcgt aactatcagt ctaacgccca
    30181 gaccatcaaa acccaggacc agatcctcaa cacgctggtt aacttacgct aatcgctgac
    30241 gggatagctc aatggatcac gcaatttata ccgcgatggg agcagccagc cagacgctga
    30301 atcaacaggc ggtaaccgcc aataatctgg ccaatgcctc aacgcccggt tttcgcgcgc
    30361 agttaaacgc cttgcgcgca gtgccggtgg aagggctttc actgcccacg cgcacgctgg
    30421 taacggcgtc aacaccgggt gcagacatga cgcccggcaa gatggattac acctcgcgcc
    30481 cgctggacgt tgcgttgcag caggatggtt ggctggccgt gcagaccgct gacggcagtg
    30541 aagggtatac gcgtaatggc agcattcagg ttgatcctac cgggcaactg acaattcagg
    30601 gacatccggt ggtaggcgag gcggggccaa ttgctgtgcc ggaaggggag gagatcacta
    30661 ttgctgccga tggcacaatc tcggcgctga atccgggcga tccggcaaat acggttgcgc
    30721 cagtggggcg tctgaagctg gtgaaagcca cgggcagcga agtccagcgc ggtgacgatg
    30781 gcatttttcg tttaagcgca gaaagccagg ctaggcgtgg gccggtactg caggcagatc
    30841 caaccttgcg tgtgatgtcg ggggttctgg aaggcagtaa cgtcaatgcc gttgcggcaa
    30901 tgagcgacat gattgccagc gcacggcgtt ttgaaatgca gatgaaggtg atcagcagcg
    30961 tcgatgataa cgcaggccgt gccaaccaac tgctgtcgat gagttaattg aaaggataca
    31021 tgacaagtat aagttgccct atgcgcaagt ttatcgggtc tatgggggca atcgcaattt
    31081 atcgattttg cgagctcttg taggccggat aaggcgttta cgccgcatcc ggcaagaaga
    31141 catatgcact ttgtcactaa tccactacag gacattttat gatcagttca ttatggatcg
    31201 ccaaaacggg ccttgacgcc cagcaaacca atatggacgt cattgccaac aacctggcaa
    31261 acgttagtac caacggtttt aagcgtcagc gcgcggtgtt tgaagatctg ctttatcaaa
    31321 ccattcgcca gccgggggca cagtcttccg aacaaaccac cttaccatcc ggattacaaa
    31381 tcggtacggg ggtacgcccg gtcgccacgg aacgtttaca cagccagggc aacctgtcgc
    31441 aaaccaataa cagtaaagac gtcgcgatta aagggcaggg ctttttccag gtgatgttgc
    31501 cagatggctc atcagcctat acccgtgacg gctctttcca ggtggatcag aacgggcagc
    31561 tggtgacggc cggtggtttt caggtgcagc cagcgatcac cattccggcg aatgcgttaa
    31621 gtatcaccat cggtcgtgat ggcgtggtca gcgtaaccca acaaggccag gcagctccgg
    31681 ttcaggttgg gcagctcaat ctcaccacct ttatgaatga caccggactg gagagcattg
    31741 gcgaaaacct ctacaccgaa acgcaatcct ccggtgcacc gaacgaaagc acgccgggcc
    31801 tgaacggcgc gggactgctg tatcaagggt atgttgaaac gtctaacgtc aacgtggcgg
    31861 aagaactggt caatatgatt caggtgcaac gcgcttacga aatcaacagt aaagcggtgt
    31921 ccaccaccga tcagatgctg caaaaactga cgcaactcta aggcttaacc ggtggcaggt
    31981 tcaccggttt actgattttt gaagatgata gccatgcaaa aaaacgctgc gcatacttat
    32041 gccatttcca gcttgttggt gctttcacta accggctgcg cctggatacc ctccacgccg
    32101 ctggtacaag gggcgaccag tgcacaaccg gttcccggtc cgacgcccgt cgccaacggg
    32161 tctattttcc agtctgctca gccgattaac tatggctatc aaccgctgtt tgaagatcgt
    32221 cgaccacgca atattggcga tacgctgact atcgtgttgc aggagaacgt cagcgccagc
    32281 aaaagctcct ctgcgaatgc cagccgtgac ggtaaaacta attttggctt tgatactgtg
    32341 ccgcgctatt tgcaggggtt gtttggtaac gctcgtgccg atgtcgaagc ctccggtggt
    32401 aacacgttca acggaaaagg cggggccaat gccagcaata cctttagcgg tacgttgacg
    32461 gtgacggttg accaggtact ggtcaacggc aacctgcatg tggtgggtga aaaacagatc
    32521 gccattaatc aaggtaccga atttattcgc ttctctggcg tggttaatcc acgcactatc
    32581 agcggcagca ataccgtacc atctactcag gtggcggatg cgcgcattga atacgtaggc
    32641 aatggctaca ttaacgaagc gcaaaatatg ggctggttgc agcgtttctt ccttaacctg
    32701 tcgccaatgt aagtgaggct gtggtgatta aatttctctc tgcattaatt cttctactgg
    32761 ttatcacggc ggctcaggct gagcgtattc gcgatctcac cagtgttcag ggggtaaggc
    32821 aaaactcact gattggctat ggcctggtgg tggggctgga tggaaccggt gaccagacaa
    32881 cccagacgcc gtttaccaca caaacgctta ataacatgct ctcacagctg ggaattaccg
    32941 ttccgacggg caccaatatg cagctaaaaa acgtcgctgc ggtaatggtg acagcgtcac
    33001 ttccgccgtt tggacgccag gggcaaacca tcgatgtggt ggtttcttcc atgggtaatg
    33061 ccaaaagctt gcgtggcggt acgttgttga tgacaccgct taagggcgtt gacagtcagg
    33121 tgtatgcgct ggcgcagggc aatattctgg ttggtggcgc tggagcctcc gctggcggta
    33181 gcagtgttca ggtgaaccaa ctgaacggtg gacggatcac caatggtgcg gttattgaac
    33241 gtgaattgcc cagccagttt ggcgtcggga atacccttaa tttgcaactt aacgacgaag
    33301 atttcagcat ggcgcagcaa atcgctgaca ccatcaaccg cgtgcgtgga tatggcagcg
    33361 ccaccgcgtt agatgcgcgg actattcagg tgcgcgtacc aagtggcaac agttcccagg
    33421 tccgtttcct tgccgatatc cagaatatgc aggttaatgt caccccgcag gacgctaaag
    33481 tagtgattaa ctcgcgcact ggttcggtgg tgatgaatcg cgaagtgacc ctcgacagct
    33541 gcgcggtagc gcaggggaat ctctcagtaa cagttaatcg tcaggccaat gtcagccagc
    33601 cagatacacc gtttggtggc gggcagaccg tggtaacgcc acaaacgcag atcgatttac
    33661 gccagagcgg cggttcgctg caaagcgtac gttccagcgc cagcctcaat aacgtggtgc
    33721 gtgcgctcaa tgcgctgggc gctacgccga tggatctgat gtccatactg caatcaatgc
    33781 aaagtgcggg atgtctgcgg gcaaaactgg aaatcatctg atgatcagcg acagcaaact
    33841 actggcaagt gcggcctggg atgcacaatc gctcaacgaa cttaaggcga aagcgggcga
    33901 agatccggcg gcaaatatcc gtccggtggc ccgccaggtg gaagggatgt tcgtgcagat
    33961 gatgttgaaa agcatgcgcg acgctttacc aaaagatggc ctgttcagca gcgagcgcac
    34021 tcgcctgtat accagtatgt atgaccagca gattgcccaa cagatgacgg cgggcaaagg
    34081 tctggggctg gcagagatga tggttaaaca gatgacgcca gaacaaccat tgccagagga
    34141 gtccacgcca gcagcaccga tgaaattccc gctcgaaacc gtggtgcgtt atcaaaatca
    34201 gacgctttcg cagctggtgc aaaaggccgt accacgtaac tacgatgatt cgctgccggg
    34261 tgacagtaaa gcattcctcg cgcaactctc gttgcccgcc caactggcaa gccagcaaag
    34321 cggtgtgcca catcatttga tcctcgctca ggcggcgctt gaatctggct ggggacaacg
    34381 gcaaatccgc cgtgaaaacg gcgagccgag ctataacctg tttggcgtca aagcctctgg
    34441 caactggaaa gggccagtca ctgaaatcac cacgactgaa tatgaaaatg gcgaagcgaa
    34501 gaaagtaaaa gcgaagtttc gggtctacag ctcgtatctg gaagcattgt cggattacgt
    34561 tgggctgtta acgcgtaacc cgcgctacgc cgccgtgacg accgccgcga gtgcggagca
    34621 gggggcgcag gccctacagg acgcgggcta tgccaccgat cctcactatg cccgcaaact
    34681 caccagcatg attcagcaga tgaaatcgat aagcgacaag gtgagcaaaa cctacagcat
    34741 gaacattgat aatctgttct gaataactca agtccggcgg gtcgctgccg ataatactct
    34801 gtaattgaag gcttataagg aacctccatg tccagcttga tcaataacgc catgagcgga
    34861 ctgaacgcgg cccaggcggc gttaaatacg gcaagtaata atatctccag ctataacgtt
    34921 gccggatata cccgccaaac cactattatg gcgcaggcca atagcacgtt gggcgctggc
    34981 ggctgggttg gcaatggcgt ctacgtttct ggtgtgcagc gtgagtatga tgcgtttatt
    35041 accaaccagt tacgtgctgc gcagacgcaa agtagcggcc tgactgcccg ctatgagcag
    35101 atgtcgaaaa tcgacaatat gctctccacc agtacctctt cgctggcaac acagatgcag
    35161 gatttcttca ccagcctgca aacgctggtg agtaacgcgg aagacccggc agcgcgccag
    35221 gcgctgattg ggaaatcaga agggttggtg aatcagttta aaaccaccga tcaatatctg
    35281 cgcgaccagg acaaacaggt caatatcgcg ataggtgcca gcgttgatca gatcaacaat
    35341 tacgctaaac aaatcgccag cctgaacgat caaatctcgc gtctgacagg cgtgggggca
    35401 ggggcgtcac ctaacaatct gctggatcaa cgcgatcaac tggtgagtga attaaaccag
    35461 attgttggcg tagaagttag cgttcaggat ggtggcactt ataacatcac gatggccaat
    35521 ggctactcac tggttcaggg aagtacggcg cggcagctgg cggcagttcc ttccagcgct
    35581 gacccttctc gtacgactgt cgcttacgtt gatggtacgg caggcaatat tgagatcccg
    35641 gagaaattgc tgaataccgg gtcgctgggc ggcattctga cattccgttc tcaggatctg
    35701 gaccagacgc gtaatacgct cggacaactg gcgctggcat ttgccggggc tttcaacacc
    35761 caacacaaag ccggatttga tgctaacggc gatgccggtg aagatttctt tgctatcggt
    35821 aagcccgcgg ttctgcaaaa cacgaaaaac aacggtgacg ttgcgatcgg tgcaacggta
    35881 actgatgcct ccgcggtact ggcgacagat tacaaaatct cgttcgataa taatcagtgg
    35941 caggtcaccc gccttgccag caataccact tttacggtga cgccggatgc caacggtaaa
    36001 gtggcatttg atggtctgga gttgacgttt acaggaacgc ctgccgttaa cgacagcttc
    36061 acgttgaaac cggtaagtga cgccatcgtc aacatggatg tattaatcac tgacgaagcg
    36121 aaaatcgcca tggcgagcgc agaagatgcg ggtgatagcg acaaccgcaa cggtcaggcc
    36181 ctgctggatc tgcaaagcaa cagtaaaacg gtgggcggtg cgaaatcctt taacgacgct
    36241 tatgcctcgt tagtgagtga tatcggtaat aaaaccgcga cgttgaaaac cagtagcgcc
    36301 acgcaaggta atgtggtgac gcagctttcc aatcagcagc agtcgatttc cggtgtcaat
    36361 ctcgatgagg agtacggaaa tctgcaacgt tttcagcagt attacctggc gaatgcgcag
    36421 gttctgcaga cggcaaacgc gatttttgat gcgctgatta acattcgcta aggggagata
    36481 aaatgcgttt cagtacccag atgatgtacc agcaaaacat gcgcggcatc accaattctc
    36541 aggcagaatg gatgaagtac ggtgaacaga tgtcgacggg taagcgagtc gttaaccctt
    36601 cagatgatcc cattgctgca tcacaagctg ttgttctctc ccaggcacag gcgcaaaaca
    36661 gccagtacac gctggcgcgt actttcgcca ctcaaaaagt gtcactggaa gagagtgtac
    36721 ttagccaggt caccaccgct atccagaatg ctcaggaaaa aattgtctac gccagcaatg
    36781 gcaccttgag tgacgatgac cgggcctcgc tggctacgga tattcagggg cttcgtgacc
    36841 agttgctgaa tctggcgaac accactgacg gtaacgggcg ctacattttt gccggttata
    36901 aaacagaatc cgcaccgttt agcgaggcgg atggcaaata cgaaggcgga gcagaaagta
    36961 ttaaacaaca ggtcgatgct tcgcgttcga tggtgatagg gcacacgggt gacaaaattt
    37021 tcgacagtat taccagcaac gcggtagcgg aaccagacgg tagcgcttct gaaaccaatc
    37081 tttttgccat gctggatagc gccatcgcag ccctgaaaac gccggtcgcg gatagcgaag
    37141 cggataaaga aaccgccgct gcggcactgg ataaaaccaa ccgcggactg aaaaactcgc
    37201 tgaacaatgt gctgactgtt cgcgcggaat taggcacgca gctgaacgaa ctggagtcgc
    37261 tggattcctt aggtagcgat cgcgctttag ggcaaacgca gcagatgagc gatctggttg
    37321 atgtggactg gaatgcaact atttcatctt acatcatgca gcaaacggca ttgcaggcgt
    37381 cgtataaagc atttaccgat atgcagggat tgtcgctctt ccagctcaac aaataatttc
    37441 gctttaaaac atatcatgaa actgggtatg ttttgtctgc ctgctccggg atcgccgggg
    37501 cgggcatttt tttgcctatt ttgtattgtt ggttagcaag gatgccattt gatgaatttt
    37561 aatatgttga ctcaaagatg aaataaaaaa gccctggtag ttaccagggc ttgattactt
    37621 gagctaatta ttactcaaca ggttgcggac gcgcaggagc ggcagatgca tgatgcgttg
    37681 ccgtatgacc acctgcggca cctttacctt cgaaggcgaa agtagggcgc tgccagtcac
    37741 tgtgacgcgg tgcttccgga acatattccg gtgccggagc gcgcgtcatc ggcgcggtag
    37801 cgtggttatg ctcaacggtg acctcaggtt caaccgcagc taccgtttca acttctgctg
    37861 cgacttcagc gacgactggc gcggcaggtt gagcaacaac ttcaggttca gcaaccacaa
    37921 cctcagcagt ttcggcaact tcttcaatat ctgccgtatc ttcctgcggt tcaaccaccg
    37981 gttctgcgtg ttctgcaact tcctgggcta cggcaacatc agactcggta atcacctgcg
    38041 gctgttcagt taccgcggca gcaatcactt caggatgcgt cgtttcaacc acttcaggtt
    38101 gcggttcggc aacctgaacg ggggcttcta cgacttcagc cacttcttca acaactggcg
    38161 cgctaacaac aggtttgaca gcggcggcga cagggacctc agtcaccatc ggctgcactt
    38221 gtacttcttc ctgttcgtgc tgctcttcaa cctgtacatc ttgcggacgt acaattggat
    38281 agcggatcca gactttgcca gaggccagtt ccggagacgc gcacgctacg gtcaacggca
    38341 ttggcgactg ggttggataa cgctcgtcac gatagcgacg acgacgctga ccacttacgc
    38401 gcaggtgacg aggcgagcgg cgagaacgac gcggcatgcc accgttgtca cggttatcgg
    38461 cattgttctc ttcttgctgt tctggtgcag cttgcgctac gactggcagc gggactttca
    38521 ccagttctgt gcgtggagct ggcgcttcct gaacaattgg ttcggcagcg acagtttctt
    38581 caaccaccgg tgcgactacc gcttcttcgg ctacgctttg ctcgtaacgc actttctgat
    38641 tgagctgacg ctgtttacga cgcggctgaa ccggacgtac acgttcttcc tgttcggttt
    38701 cctgaacaga ttgctcttca acattcagcg ccttcgcttc ttgttgcgcc tgacgtttat
    38761 catcgttacg gcggcggcta cgttcacgac gcggcgcttg ctgctcgtcg gtggtacgcg
    38821 ctttttccgt tacctcagcc tgctgacggc tctcacgcgt ctcgacagtc tgctgctgtg
    38881 cctggcgacg attacgacgg ttttcttcgc gattatcgct gccttctgta cgttcactac
    38941 gggtatcgcg gcgctcatta cggtcacggc ggttgttctg acgaggcttg cgacgatcct
    39001 gttgacgttc cggtttcgct tctgctttcg gtgctggttg ctcggccggt ttggtttctt
    39061 caccaccgct gaacagcgct ttcagtgcgc cgaagaagcg gctcaacaac cctggttgtg
    39121 caggagatgc tggtgttgcc gttgcagctt taggtgctgg ggctacaaca ggcgcggcag
    39181 gttcagctgg ggttggagct ggcggcacat ccggcatggc aaaggttgcc agcgcaggtt
    39241 gttccggacg cttacgttca gcgaactctt cttcagacgg cagcgccatc gcttcttcat
    39301 gcagcttcgg cagcatgtag cttaaggttg gagtttcttc ccctttacgc acgcgcagca
    39361 catggtagtg cggggtttcc atctgatcgt ttggcacaat cacacagcgc acgccgtcct
    39421 gacgagtttc aatcgcattc actgcagaac gtttttcatt cagcaggtaa gacgcgattg
    39481 gcacaggaac aatggcgtga acttcctgag tgttctcttt cagcgcttct tcttcgatca
    39541 gacgcagaat agagagcgac aacgattcgt tgtcacgcac ggtaccggta ccggagcagc
    39601 gcgggcagac gtgatggctg gattcaccca gtgatgggct cagacgctga cgggacattt
    39661 ccagcaggcc aaagcgagaa atatggctga tttgaatacg cgcacggtcc tgacgcaccg
    39721 cttcacgcag acgattttct accgcacgct ggtggcgtac aggcgtcatg tcgatgaagt
    39781 cgataacaat caggccgccg aggtcacgca ggcgcagctg acgagcaatc tcatcggcag
    39841 cttcgaggtt agtgttaaac gcggtttctt cgatatcgcc gccgcgggtc gcgcgtgcgg
    39901 agttgatgtc gatggccgtt aacgcttcgg tgctgtcgat aacaatcgaa ccaccggatg
    39961 gcagacgaac ttcacgctgg aaggcggact cgatctgcga ctcgatctgg tagtggctga
    40021 acagcgggat ctcgccggtg tacagtttga ttttgctgct gaaatccggg cggcctaatg
    40081 cagcgatatg ctgacgtgcc agttcgagca ctttcgggtt atcgataagg atttcaccga
    40141 tgtcctggcg taagtaatcg cggaatgcgc gaacgattac gttgctctcc tgatgaatca
    40201 ggaacggagc cgggcggctt tcagcggctt ttttgatggc ttcccagtgt ttcagacgga
    40261 agcttaaatc ccattgcagc gcctcagcag atttgccgac accagcggtg cgcacgataa
    40321 gccccatgcc ttccggcagt tcaaggcttg ccagtgcttc ttttaattcg gtacggtcgt
    40381 cgccttcgat acgacgagaa ataccgcccg cgcgcgggtt gttcggcatc agaaccagat
    40441 agctacccgc cagactgata aaggtggtta atgccgcgcc tttgttgccg cgctcttctt
    40501 tatcgatctg aacaatgact tcctgacctt cacgcaacac atctttaatg ttgggacgac
    40561 catgagcact gtagttagca gggaaatatt cgcgggcaat ttcttttagt gggaggaaac
    40621 cgtgacgttc agcgccgtaa tcaacaaaag cagcttccag actcggttca atgcgggtga
    40681 ttttaccttt gtagatgttt gcctttttct gctcgtgccc tggactttcg atatccaggt
    40741 catacagacg ctgcccatct acaagggcaa cgcgcaactc ttcctgctga gttgcgttga
    40801 ttaacattct tttcatcgta acttactcat tattcttaca ttgacgacta agctgcgggc
    40861 aaagtaacgc ctttccgggt gtgaaccgat ggcctcgtgt ctagtcgcgt cgccaacctc
    40921 acggttatcg tcagctcaaa gaggcgcaga gtgtcggttg cccgtttttc atgcggaaaa
    40981 acagcgcaat tatcagagaa acagactggg tattactctc cagaaatatt tccatctacc
    41041 ggtaaggact gcaacccgca gcccgctaac tgcctgaaag atcaatacgt cttacgccat
    41101 tgctgcgtgg atgatcggtc gggcaaaatg ggttattccg taaaatttct tgttttaaca
    41161 aggatggaca cggaaacggc ctcattattc cactgcaagc cttgttatag caagatgact
    41221 tttaccattt atcacccgct tactcacagt tttttcactt cttgctggtg attggtttaa
    41281 taaccatcaa taaagtgatc acctagtgaa gtaataaggt gtaaaactaa atataagcat
    41341 agaaaaatga gtggcgcgaa tcctgttggc taattagaat cgcccaccat gaaaacagag
    41401 actccatccg taaaaattgt tgctatcacc gctgacgaag cggggcaacg tatcgacaac
    41461 tttttgcgta cccaattgaa aggcgtacca aaaagtatga tttaccgtat tttgcgtaaa
    41521 ggcgaagtgc gggtgaacaa aaaacgtatt aagcctgaat ataaactcga agcgggtgat
    41581 gaggtgcgta tcccaccggt tcgcgttgct gagcgggaag aagaggcggt ttcgccacat
    41641 ctgcaaaagg tggcggcgct ggcggacgtc atcttatatg aagatgatca tatcctggtg
    41701 ctgaataaac cttccggtac ggcggtacat ggcggcagtg gtttaagctt cggcgttatt
    41761 gaaggtttgc gggcgttgcg cccggaagcg cggttccttg aactggttca tcgtcttgac
    41821 cgggacacct caggtgtgtt gctggtagcg aaaaaacgct cggcgttgcg ttctctgcat
    41881 gagcaattac gtgaaaaagg gatgcaaaaa gattacctgg cgctggtgcg cggtcagtgg
    41941 cagtcgcatg tgaagagcgt tcaagcgccg ttactgaaaa atattctgca aagcggcgaa
    42001 cgtatcgtgc gtgtgagtca ggaaggcaaa ccgtcggaaa cacgctttaa agtggaagag
    42061 cgctatgcat ttgccaccct ggtgcgttgt agcccggtaa cagggcgtac tcatcagatc
    42121 cgtgtgcata cacaatatgc gggtcatccg attgcctttg acgatcgcta cggtgacggt
    42181 gaatttgaca gacagctcac tgaagcaggc acgggattaa atcgcctgtt cctgcacgcc
    42241 gcagcgttga agtttactca tccggggacc ggtgaggtga tgcgtattga agcgccgatg
    42301 gatgaaggtt tgaagcgttg tttgcaaaag ctgcgtaacg cgcgctaata caagcatata
    42361 aacctgataa tgggcggttg gcctgataag gcgtttacgc cgcatcaggc cgacagcacc
    42421 gattgccgga tgcgaagtaa ccgcatccgg cgcaaaaaac tatcccatca gcgggttttt
    42481 cccttcccga cgtaacatct gacacagcgc gattagcggt aaaccaacca gcgtgttagg
    42541 atcacgcccc tctaaacgct caaacagcgt aatgccaaat ccttcactct taaagctacc
    42601 cgcgcagtgc aggggatgct ctttacgcac gtaattatca atctccgcct cgctcaggtg
    42661 acggaaatgg acgtcaaaag gctccacttc tgtttgtaga tgcccattcg ccgaattaaa
    42721 caacgccagt ccagtataga aggtgacgat attgccgctg gctttgcgta attgcagacg
    42781 ggcattttct tccgttaacg gtttaccggt gatttcgcca tcaagaacac atacctggtc
    42841 tgaaccaata attaaatgat ccggataacg tgacgccaga gattgcgctt tttcttgtgc
    42901 cagtcgaagc accaactgtc gcggtgattc gtcgctgcgt ggagtttcgt cgacctcggg
    42961 tgctgcacat tcaaaagaga tttgcagttt ttccagaaga gcgcgacgcc agggcgaggt
    43021 ggaggctaaa ataagtttag gcatattttt ttccatcaga tatagcgtat tgatgatagc
    43081 cattttaaac tatgcgcttc gttttgcagg ttgatgttcg ttatcagcac tgaacgaaaa
    43141 taaagcagta acccgcaatg tgtgcgaatt attggcaaaa ggcaaccaca ggctgccttt
    43201 ttctttgact ctatgacgtt acaaagttaa tatgcgcgcc ctatgcaaaa ggtaaaatta
    43261 cccctgactc tcgatccggt tcgtacggct caaaaacgcc ttgattacca gggtatctat
    43321 actcctgatc aggttgagcg cgtcgccgaa tccgtagtca gtgtggacag tgatgtggaa
    43381 tgctccatgt cgttcgctat cgataaccaa cgtctcgcag tgttaaacgg cgatgcgaag
    43441 gtgacggtaa cgctcgagtg tcagcgttgc gggaagccgt ttactcatca ggtctacaca
    43501 acgtattgtt ttagtcctgt gcgttcagac gaacaggctg aagcactgcc ggaagcgtat
    43561 gaaccgattg aggttaacga attcggtgaa atcgatctgc ttgcaatggt tgaagatgaa
    43621 atcatcctcg ccttgccggt agttccggtg catgattctg aacactgtga agtgtccgaa
    43681 gcggacatgg tctttggtga actgcctgaa gaagcgcaaa agccaaaccc atttgccgta
    43741 ttagccagct taaagcgtaa gtaattggtg ctccccgttg gatcggggat aaaccgtaat
    43801 tgaggagtaa ggtccatggc cgtacaacag aataaaccaa cccgttccaa acgtggcatg
    43861 cgtcgttccc atgacgcgct gaccgcagtc accagcctgt ctgtagacaa aacttctggt
    43921 gaaaaacacc tgcgtcacca catcactgcc gacggttact accgcggccg caaggtcatc
    43981 gctaagtaat cacgcgtctg cgtgatgaag cttagtgagg attttcccca ggcaactggg
    44041 gaaagaccaa accgggcggc gacgatacct tgacacgtct aaccctggcg ctagatgtca
    44101 tgggagggga ttttggccct tccgtgacag tgcctgcagc attgcaggca ctgaattcta
    44161 attcgcaact cactcttctt ttagtcggca atcccgacgc catcacgcca ttacttgcta
    44221 aagctgactt tgaacaacgt tcgcgtctgc agattattcc tgcgcagtca gttatcgcca
    44281 gtgatgcccg gccttcgcaa gctatccgcg ccagtcgtgg gagttcaatg cgcgtggccc
    44341 tggagctggt gaaagaaggt cgagcgcaag cctgtgtcag tgccggtaat accggggcgc
    44401 taatggggct ggcaaaatta ttactcaagc ccctggaggg gattgagcgt ccggcgctgg
    44461 tgacggtatt accgcatcag caaaagggca aaacggtggt cctcgattta ggggccaacg
    44521 tcgattgtga cagtacaatg ttggtgcaat ttgccattat gggctcagtt ctggctgaag
    44581 aggtggtgga aattcctaat cctcgcgtgg cgttgctcaa tattggtgaa gaagaagtaa
    44641 agggtctcga cagtattcgg gatgcctcag cggtgcttaa aacaatccct tctatcaatt
    44701 atatcggcta tcttgaagcc aatgagttgt taactggcaa gacagatgtg ctggtttgtg
    44761 acggctttac aggaaatgtc acattaaaga cgatggaagg tgttgtcagg atgttccttt
    44821 ctctgctgaa atctcagggt gaagggaaaa aacggtcgtg gtggctactg ttattaaagc
    44881 gttggctaca aaagagcctg acgaggcgat tcagtcacct caaccccgac cagtataacg
    44941 gcgcctgtct gttaggattg cgcggcacgg tgataaaaag tcatggtgca gccaatcagc
    45001 gagcttttgc ggtcgcgatt gaacaggcag tgcaggcggt gcagcgacaa gttcctcagc
    45061 gaattgccgc tcgcctggaa tctgtatacc cagctggttt tgagctgctg gacggtggca
    45121 aaagcggaac tctgcggtag caggacgctg ccagcgaact cgcagtttgc aagtgacggt
    45181 atataaccga aaagtgactg agcgtacatg tatacgaaga ttattggtac tggcagctat
    45241 ctgcccgaac aagtgcggac aaacgccgat ttggaaaaaa tggtggacac ctctgacgag
    45301 tggattgtca ctcgtaccgg tatccgcgaa cgccacattg ccgcgccaaa cgaaaccgtt
    45361 tcaaccatgg gctttgaagc ggcgacacgt gcaattgaga tggcgggcat tgagaaagac
    45421 cagattggcc tgatcgttgt ggcaacgact tctgctacgc acgcttttcc gagcgcagct
    45481 tgtcagattc aaagcatgct gggcattaaa ggttgcccgg catttgacgt tgcagcagcc
    45541 tgcgcaggtt tcacctatgc attaagcgta gccgatcaat acgtgaaatc tggggcggtg
    45601 aagtatgctc tggtcgtcgg ttccgatgta ctggcgcgca cctgcgatcc aacagaccgt
    45661 gggactatta ttatttttgg cgatggcgcg ggcgctgcgg tgctggctgc ctctgaagag
    45721 ccgggaatca tctccaccca tctgcatgcc gacggtagct atggtgaatt gctgacgctg
    45781 cctaatgctg accgtgtgaa tccagagaat tcaattcatc tgacaatggc gggtaacgaa
    45841 gtcttcaagg ttgcggtaac ggaactggcg cacatcgttg atgagacgct agcggcgaat
    45901 aatcttgacc gttctcaact ggactggctg gttccgcatc aggctaacct gcgtattatc
    45961 agtgcaacgg cgaaaaaact cggcatgtcg atggacaatg ttgtggtgac gctggatcgc
    46021 cacggtaata cctctgcggc ctctgtcccg tgcgcgctgg atgaagctgt acgtgacggg
    46081 cgcattaagc cgggacagtt ggttctgctt gaagcctttg gcggtggatt cacctggggc
    46141 tccgcgctgg ttcgtttcta ggataaggat taaaacatga cgcaatttgc atttgtgttc
    46201 cctggacagg gttctcaaac cgttggaatg ctggctgata tggcggcgag ctatccaatt
    46261 gtcgaagaaa cgtttgctga agcttctgcg gcgctgggct acgacctgtg ggcgctgacc
    46321 cagcaggggc cagctgaaga actgaataaa acctggcaaa cccagccagc gctgttgact
    46381 gcatctgttg cgctgtatcg cgtatggcag cagcagggcg gtaaagcacc ggcaatgatg
    46441 gccggtcaca gcctggggga atactccgcg ctggtttgcg ctggtgtgat tgatttcgct
    46501 gatgcggtgc gtctggttga gatgcgcggc aagttcatgc aagaagccgt accggaagga
    46561 acgggcgcta tggcggcaat catcggtctg gatgatgcgt ctattggaaa agcgtgtgaa
    46621 gaagctgcag aaggtcaggt cgtttctccg gtaaacttta actctccggg acaggtggtt
    46681 attgccggtc ataaagaagc ggttgagcgt gctggcgctg cctgtaaagc tgcgggcgca
    46741 aaacgcgctc tgccgttacc agtgagcgta ccatctcact gtgcgctgat gaaaccagca
    46801 gctgacaaac tggcagtaga attagcgaaa atcaccttta acgcaccaac agttcctgtt
    46861 gtgaataacg ttgatgtgaa atgcgaaacc aatggtgatg ccattcgtga cgcactggta
    46921 cgtcagttgt ataacccggt tcagtggacg aagtctgttg agtacattgc agcgcaaggc
    46981 gtagaacatc tctatgaagt cggcccgggc aaagtgctta ctggcctgac gaaacgcatt
    47041 gtcgacaccc tgaccgcctc ggcgctgaac gaaccttcag cgatggcagc ggcgctcgag
    47101 ctttaaaaga ggaaaatcat gaattttgaa ggaaaaatcg cactggtaac cggtgcaagc
    47161 cgcggaattg gccgcgcaat tgctgaaacg ctcgcagcct gtggcgcgaa agttattggc
    47221 actgcgacca gtgaaaatgg cgctcaggcg atcactgatt atttaggtgc taacggcaaa
    47281 ggtctgatgt tgaatgtgac cgacccggca tctatcgaat ctgttctgga aaaaattcgc
    47341 gcagaatttg gtgaagtgga tatcctggtc aataatgccg gtatcactcg tgataacctg
    47401 ttaatgcgaa tgaaagatga agagtggaac gatattatcg aaaccaacct ttcatctgtt
    47461 ttccgtctgt caaaagcggt aatgcgcgct atgatgaaaa agcgtcatgg tcgtattatc
    47521 actatcggtt ctgtggttgg taccatggga aatggcggtc aggccaacta cgctgcggcg
    47581 aaagcgggct tgatcggctt cagtaaatca ctggcgcgcg aagttgcgtc acgcggtatt
    47641 actgtaaacg ttgttgctcc gggctttatt gaaacggaca tgacacgtgc gctgagcgat
    47701 gaccagcgtg cgggtatcct ggcgcaggtt cctgcgggtc gcctcggcgg cgcacaggaa
    47761 atcgccaacg cggttgcatt cctggcatcc gacgaagcag cttacatcac gggtgaaact
    47821 ttgcatgtga acggcgggat gtacatggtc tgaccgcgat ttgcacaaaa tgctcatgtt
    47881 gcgcgcagtc tgcgtggtta tgagtaataa ttagtgcaaa atgatttgcg ttattggggg
    47941 gtaaggcctc aaaataacgt aaaatcgtgg taagacctgc cgggatttag ttgcaaattt
    48001 ttcaacattt tatacactac gaaaaccatc gcgaaagcga gttttgatag gaaatttaag
    48061 agtatgagca ctatcgaaga acgcgttaag aaaattatcg gcgaacagct gggcgttaag
    48121 caggaagaag ttaccaacaa tgcttctttc gttgaagacc tgggcgcgga ttctcttgac
    48181 accgttgagc tggtaatggc tctggaagaa gagtttgata ctgagattcc ggacgaagaa
    48241 gctgagaaaa tcaccaccgt tcaggctgcc attgattaca tcaacggcca ccaggcgtaa
    48301 gtgaacatct ccaggcggtc gttcgaccgc ctgagtttta tctttttgtc ccactagaat
    48361 cattttttcc ctccctggag gacaaacgtg tctaagcgtc gtgtagttgt gaccggactg
    48421 ggcatgttgt ctcctgtcgg caataccgta gagtctacct ggaaagctct gcttgccggt
    48481 cagagtggca tcagcctaat cgaccatttc gatactagcg cctatgcaac gaaatttgct
    48541 ggcttagtaa aggattttaa ctgtgaggac attatctcgc gcaaagaaca gcgcaagatg
    48601 gatgccttca ttcaatatgg aattgtcgct ggcgttcagg ccatgcagga ttctggcctt
    48661 gaaataacgg aagagaacgc aacccgcatt ggtgccgcaa ttggctccgg gattggcggc
    48721 ctcggactga tcgaagaaaa ccacacatct ctgatgaacg gtggtccacg taagatcagc
    48781 ccattcttcg ttccgtcaac gattgtgaac atggtggcag gtcatctgac tatcatgtat
    48841 ggcctgcgtg gcccaagcat ctctatcgcg accgcctgta cttccggcgt gcacaacatt
    48901 ggccatgctg cgcgtattat cgcgtatggc gatgctgacg tgatggttgc aggtggcgca
    48961 gagaaagcca gtacgccgct gggcgttggt ggttttggcg cggcacgtgc attatctacc
    49021 cgcaatgata acccgcaagc ggcgagccgc ccgtgggata aagagcgtga tggtttcgta
    49081 ctgggcgatg gtgccggtat gctggtactt gaagagtacg aacacgcgaa aaaacgcggt
    49141 gcgaaaattt acgctgaact cgtcggcttt ggtatgagca gcgatgctta tcatatgacg
    49201 tcaccgccag aaaatggcgc aggcgcagct ctggcaatgg caaatgcttt gcgtgatgca
    49261 ggcattgaag cgagtcagat tggctacgtt aacgcacacg gtacttctac tccggctggc
    49321 gataaagctg aagcgcaggc ggtgaaaacc atcttcggtg aagctgcaag ccgtgtgttg
    49381 gtaagctcca cgaaatctat gaccggtcac ctgttaggtg cggcgggtgc agtagaatct
    49441 atctactcca tcctggcgct gcgcgatcag gctgttccgc caaccatcaa cctggataac
    49501 ccggatgaag gttgcgatct ggatttcgta ccgcacgaag cacgtcaggt cagcggaatg
    49561 gaatacacgc tgtgtaactc cttcggcttc ggtggcacta atggttcttt gatctttaaa
    49621 aagatctaag ttgtcatttc ccacccttat aaaaggtccg cttgcgggcc ttttttctta
    49681 gcttttattc cgacttgttc cgtagtgaac atgctgccac actaacaatt ctatgataag
    49741 gagctggtat gttcttaatt aacggtcata agcaggaatc gctggcagta agcgatcggg
    49801 caacgcagtt tggtgatggt tgttttacca ccgccagagt tatcgacggt aaagtcagtt
    49861 tgttatcggc gcatatccag cgactacagg atgcttgtca gcggttgatg atttcctgtg
    49921 acttctggcc tcagcttgaa caagagatga aaacgctggc agcagaacag cagaatggtg
    49981 tgctgaaagt cgtgatcagt cgcggtagtg gcgggcgagg gtacagcaca ttgaacagtg
    50041 gaccggcaac gcggattctc tccattacgg cttatcctgc acattacgac cgtttgcgta
    50101 acgaagggat gatgttggcg ctaagcccgg tgcggctggg gcgcaatcct caccttgcag
    50161 gtattaaaca tcttaatcgg cttgagcaag tattgatccg ctctcatctt gagcagacaa
    50221 acgctgatga ggcgctggtc cttgacagcg aagggtgggt tacggaatgc tgtgcggcta
    50281 atttgttctg gcggaagggc aacgtagttt atacgccgcg actggatcag gcaggtgtta
    50341 acggcattat gcgacaattc tgtatccgtt tgctggcaca atcctcttat cagcttgtcg
    50401 aagtgcaagc ctctctggaa gaggcgttgc aggcagatga gatggttatt tgtaatgcgt
    50461 taatgccagt gatgcccgtg cgtgcctgtg gcgatgcctc cttttcgtca gcaacgttat
    50521 atgaatattt agccccactt tgtgagcgcc cgaattagtc atgaaaaaag tgttattgat
    50581 aattttgtta ttgctggtgg tactgggtat cgccgctggt gtgggcgtct ggaaggttcg
    50641 ccatcttgcc gacagcaaat tgcttatcaa agaagagacg atatttaccc tgaagccagg
    50701 gaccggacgt ttggcgctag gtgaacagct ttatgccgat aagatcatca atcgcccacg
    50761 ggtttttcaa tggttgctgc gtatcgaacc ggatctttct cactttaaag ccgggactta
    50821 ccgctttaca ccgcagatga ccgtgcgcga gatgctgaaa ttgttggaaa gcggtaaaga
    50881 agcacagttc ccgctgcgac tggtagaagg gatgcgtctg agcgactacc tcaagcaatt
    50941 gcgtgaggct ccgtatatca agcacacgct gagcgacgat aagtacgcca ccgtagcgca
    51001 ggcacttgaa ctggaaaacc cggagtggat tgaaggttgg ttctggccag acacctggat
    51061 gtataccgcc aataccaccg atgtcgcgtt actcaagcga gcgcacaaga aaatggtgaa
    51121 agcggtcgat agcgcctggg aagggcgtgc ggacggtctg ccttataaag ataaaaacca
    51181 gttggtgacg atggcatcaa ttatcgaaaa agaaaccgcc gttgccagtg aacgcgatca
    51241 ggttgcctcg gtatttatca accggttacg cattggtatg cgcctgcaga ccgacccgac
    51301 cgtgatttac gggatgggag agcgttataa tggcaaactt tctcgtgcag acctggaaac
    51361 gccgacagcg tataacacct ataccattac cggtttgccg ccaggtgcaa tagctacgcc
    51421 gggggcggat tcgctgaagg ctgctgcgca tccggcaaaa acgccgtatc tctattttgt
    51481 ggccgatggt aaaggtggtc acacgtttaa taccaatctt gccagtcata acaagtctgt
    51541 gcaggattat ctgaaagtgc ttaaggaaaa aaatgcgcag taagtatatc gtcattgagg
    51601 ggctggaagg cgcaggcaaa actaccgcgc gtaacgtggt ggttgagacg cttgagcaac
    51661 tgggtatccg cgacatggtt ttcactcggg aacctggcgg tacgcaactt gccgaaaagt
    51721 taagaagcct ggtgctggat atcaaatcgg taggcgatga agtcattacc gataaagccg
    51781 aagttctgat gttttatgcc gcgcgcgttc aactggtaga aacggtcatc aaacctgcgc
    51841 tggctaacgg cacctgggtg attggcgatc gccacgatct ctctactcaa gcctatcagg
    51901 gtggaggacg aggtattgac caacatatgc tggcaacact gcgtgatgct gttctcgggg
    51961 attttcgccc tgacttaacg ctttatcttg atgtcacccc agaggtcggc ttaaaacgtg
    52021 ctcgcgcccg tggtgagctg gatcgtattg agcaagagtc attcgatttc tttaatcgca
    52081 cccgcgcccg ctatctggaa ctggcagcac aagataaaag cattcatacc attgatgcca
    52141 cccagccgct ggaggtcgtg atggatgcaa tccgcactac cgtgaccaac tgggtgaagg
    52201 agttggacgc atgagatggt atccatggtt acgacctgat ttcgaaaaac tggtagccag
    52261 ctatcaggcc ggaagaggtc accatgcgct actcattcag gcgttaccgg gcatgggcga
    52321 tgatgcttta atctacgccc tgagccgtta tttactctgc caacaaccgc agggccacaa
    52381 aagttgcggt cactgtcgtg gatgtcagtt gatgcaggct ggcacacatc ccgattacta
    52441 caccctggct cccgaaaaag ggaaaaatgc gctgggcatt gatgcggtac gtgaggtcac
    52501 cgaaaagctg aatgagcacg cacgcttagg tggtgcgaaa gttgtctggg taaccgatgc
    52561 tgccttacta accgacgccg cggctaacgc attgctgaaa acgcttgaag agccaccagc
    52621 agagacgtgg tttttccttg ctacccgcga gccagaacgt ttactggcaa cattacgtag
    52681 tcgttgtcgg ttacattacc ttgcgccgcc gccggaacag tacgccgtga cctggctttc
    52741 acgcgaagtg acaatgtcac aggatgcact acttgccgca ttgcgcttaa gcgccggttc
    52801 gcctggggcg gcactggcgt tgtttcaggg agataactgg caggctcgtg aaacattgtg
    52861 tcaggcgctg gcatatagcg tgcaatcggg cgactggtat tcgctgctag cggcccttaa
    52921 tcatgaacaa gctccggcac gtttacactg gctggcaacg ttgttgatgg atgcgctaaa
    52981 acgccatcat ggcgctgcgc aggtgaccaa tgttgatgtg ccgggcctgg tcgtcgaact
    53041 ggcaaaccat ctttctccct cgcgcctgca ggctatactg ggggatgttt gccacattcg
    53101 tgaacagtta atgtctgtca caggcatcaa ccgcgagctt ctcatcaccg atcttttact
    53161 gcgtattgag cattacctgc aaccgggcgt tgtgctaccg gttcctcatc tttaagagag
    53221 acatcatgtt tttagtcgac tcacactgcc atctcgatgg tctggattat gaatctttgc
    53281 ataaggacgt ggatgacgtt ctggcgaaag ccgccgcacg cgatgtgaaa ttttgtctgg
    53341 cagtcgccac aacattaccg ggttatttac acatgcggga tctggtaggc gaacgcgaca
    53401 acgtcgtatt ttcttgtggc gtgcacccac tcaaccagaa tgatccctac gatgtagaag
    53461 atttacgtcg tctggcggca gaagagggcg ttgtagcgct gggtgaaacc gggctggatt
    53521 attattacac gccggaaact aaagtacgtc agcaagagtc cttcatccat catatccaga
    53581 ttggtcgtga actgaacaag ccggttatcg tccacacccg tgacgcccgc gccgatacgc
    53641 tggcaattct gcgcgaagaa aaagtgacgg attgcggtgg cgtactacac tgttttacag
    53701 aggacagaga aacggcgggt aaattactgg atctcggatt ttacatctcc ttttccggca
    53761 ttgtgacctt ccgtaatgcg gagcaactgc gcgatgctgc gcgttatgtc cccctggatc
    53821 ggttactggt ggaaactgac tcaccttacc ttgcgccggt accgcatcga ggaaaagaga
    53881 atcaacctgc gatggttcgt gacgttgcag aatacatggc tgtgttgaaa ggtgttgccg
    53941 ttgaagaact ggcgcaggta accaccgata acttcgcccg tctgtttcac atcgacgctt
    54001 cccgccttca atccatccgt tgaatgagtt tttttaaagc tcgtaattaa tggctaaaac
    54061 gagtaaagtt caccgccgaa aatcgggcgg tgaataacca cgtttgaaat attgtgacat
    54121 atgttttgtc aaaatgtgca acttctccaa tgatctgaag ttgaaacgtg atagccgtca
    54181 aacaaatcgg cactgaatta ttttactctg tgtaataaat aaagggcgct tagatgccct
    54241 gtacacggcg aggcactccc cccttgccac gcgtgagaac gtaaaaaaag cacccatact
    54301 caggagcact ctcaattatg tttaagaatg catttgctaa cctgcaaaag gtcggtaaat
    54361 cgctgatgct gccagtatcc gtactgccta tcgcaggtat tctgctgggc gtcggttccg
    54421 cgaatttcag ctggctgccc gccgttgtat cgcatgttat ggcagaagca ggcggttccg
    54481 tctttgcaaa catgccactg atttttgcga tcggtgtcgc cctcggcttt accaataacg
    54541 atggcgtatc cgcgctggcc gcagttgttg cctatggcat catggttaaa accatggccg
    54601 tggttgcgcc actggtactg catttacctg ctgaagaaat cgcctctaaa cacctggcgg
    54661 atactggcgt actcggaggg attatctccg gtgcgattgc agcgtatatg tttaaccgtt
    54721 tctaccgtat taagctgcct gagtatcttg gcttctttgc cggtaaacgc tttgtgccga
    54781 tcatttctgg cctggctgcc atctttactg gcgttgtgct gtccttcatt tggccgccga
    54841 ttggttctgc aatccagacc ttctctcagt gggctgctta ccagaacccg gtagttgcgt
    54901 ttggcattta cggtttcatc gaacgttgcc tggtaccgtt tggtctgcac cacatctgga
    54961 acgtaccttt ccagatgcaa attggtgaat acaccaacgc agcaggtcag gttttccacg
    55021 gcgacattcc gcgttatatg gcgggtgacc cgactgcggg taaactgtct ggtggcttcc
    55081 tgttcaaaat gtacggtctg ccagctgccg caattgctat ctggcactct gctaaaccag
    55141 aaaaccgcgc gaaagtgggc ggtattatga tctccgcggc gctgacctcg ttcctgaccg
    55201 gtatcaccga gccgatcgag ttctccttca tgttcgttgc gccgatcctg tacatcatcc
    55261 acgcgattct ggcaggcctg gcattcccaa tctgtattct tctggggatg cgtgacggta
    55321 cgtcgttctc gcacggtctg atcgacttca tcgttctgtc tggtaacagc agcaaactgt
    55381 ggctgttccc gatcgtcggt atcggttatg ccattgttta ctacaccatc ttccgcgtgc
    55441 tgattaaagc actggatctg aaaacgccgg gtcgtgaaga cgcgactgaa gatgcaaaag
    55501 cgacaggtac cagcgaaatg gcaccggctc tggttgctgc atttggtggt aaagaaaaca
    55561 ttactaacct cgacgcatgt attacccgtc tgcgcgtcag cgttgctgat gtgtctaaag
    55621 tggatcaggc tggcctgaag aaactgggcg cagcgggcgt agtggttgct ggttctggtg
    55681 ttcaggcgat tttcggtact aaatccgata acctgaaaac cgagatggat gagtacatcc
    55741 gtaaccacta atccgtaaga cgttggggag actaaggcag ccagatggct gcctttttta
    55801 caggcgttat tcagaattga tacgtgccgg taatgctgaa attacgcggt gcgccgtaga
    55861 cgatagaacc ttccacgttg gtatcgtagg ttttgtcgaa caggttattg acattcccct
    55921 gtaacgagaa gtttttcgtc acctggtagc gggtgaagag atccaccagc gcgtagctac
    55981 cttgctcggc gcggaaggtg ccatacggtg tcacggtgtc ggtatacacg cgattttgcc
    56041 agttaacacc accgccgacc gtcaactttg gcatgacagg caaccgatag ctggtgaaca
    56101 ttttaaccgt ggtgcgtggc agattaggat taacggcgtt tccttcgtta tcctcggcaa
    56161 tatagcgcgt tgcgccaaac gtcaactgcc agttgtcggt aattgcgccg ttgagttcaa
    56221 attccacacc tttactgact gtcccatcca ccgctttata ggcggtttcg ccgttgctgc
    56281 cggggatagg tgtaccggtg gactgagcga cattatcctg ctcaatacgg aagatggcta
    56341 acgtggtggt caggcggcta ttcatccagt ccgatttcag acccagctcg tagttgttac
    56401 cggtgattgg agccagatat ttgcctgaac tgtcacgatc atttgcggct ggaaaataga
    56461 ggtatagctg gcgtaggtcg accagttatc attgatgtca aacaccagtc cagcataagg
    56521 cgtggtgtgg tttttctcca tgctgtaagt cagcgtatca actcgccagt tggtataacg
    56581 tgcgccgagg atcagatgca gcggatcggc aagggtgaca cgagtggcag catataacga
    56641 tttcatatgt gtggtatcgt cctgcgccag gctctgtggt gaccagtcgg tttgtgggaa
    56701 attgccatta aagttgtaga aactgccaat ttcatccggg aaaatgttgg cccatgaact
    56761 gaagtaacga ttgttttgtt tgctgtaact gccaccaaac attaaattgt gctgacgacc
    56821 aaacaattca taactaccgt cagcgaacag atccagcgca tcaactttac gtttgccgct
    56881 gttccaaccg gtgcctccga cataatcaaa accagggcca taattactgt atggcccaac
    56941 cagcatacca tccgctttgt ttacataggc atcgacatac atcattttgc tgtcaaattc
    57001 gacttcagag tgggtggcat tcagcgtcgc ttgccaggta tcagcaaacc gctgcttcag
    57061 ggtcataaag accttgttga tctctttatc gttgtacgcc cagtcaggtg cggtactgcg
    57121 tgcgcgatcg taactgttgc tgctgccatc agtattccag cgcggtaaac cgccctaggt
    57181 tgggctatta acatcaatac gctgatattc gtaaccggct gaaagcatcg taagatcgcc
    57241 taaatcagca tcgacaatgc cggagaagaa ggtcttttca ctgttgtagc ggtccagcca
    57301 tgagtcgtta ttctggtagc cgccgacaat tcgtgcgcgg attttaccgt cttcggtgag
    57361 tgggctttgt aaatccgcca cataccgttc tttgttccag ctaccgtatt ccgccgagac
    57421 atcgccttta aattcacgac tggtcgcgtg ttttcgaacc atattaattg ccgcagatgg
    57481 attacccgtc ccggtcatga ggcctgtcgc gccacgcact acttctacgc gttcaaacag
    57541 tgccatatca gaaagtgcgt cgcccagatt ccagcgcgat tcaaaatagg tggggatacc
    57601 atcaaccata tagttatcga tctggaatcc gcgggaataa taaagagcac gatcggaatc
    57661 cgcctgactt ttgctgatcc ccagcgtgtt ttccatcact tcgcccagcc tttgtaactg
    57721 ctgatcttcc atccgctgct ggctaacaat agtgaccgac tgaggaatat cacgttgagt
    57781 catctgcatt tttgtacccg cagaggtaga cgttacgttg taatcatttt cgccatcatc
    57841 tggggctgtg gctgaaccct caacaatcac cgtttcttca gtagctggtg cagcaaaagc
    57901 ggcggaaggt aatagtgcca gtgctatgca accggcaagg agtgacggtt tgatgatggc
    57961 ttgatgttga ttatccctgt taaattgtgt cgaaagcatt tgaaatctct tggtttatgt
    58021 tggcgtcagg ctcatgccga agcagggtga tgagcggtta tatttagtgt gcgaataatt
    58081 tttgtgtgta aatgcaaatg agaaatatac gcattcatat ttggagtgta aaccaaacag
    58141 agaatgtctt ttcagcgcat tcgcaggcag aaatgggaat aatggcgata tatacggcag
    58201 caaaacgatt ttttgcttaa gaaatcgtga gttaaggttg aaagagcagg tttaactcga
    58261 ccatactcta tactcgcagt gtggcgcggc gtagcatggc gcaacgcatg gctatttgaa
    58321 aaaggaaaat gtcgtggcag aagaaactat attcagcaaa attattcgtc gtgagatccc
    58381 ctccgatatc gtctaccagg atgatctggt aacggcgttt cgcgatattt cgccgcaagc
    58441 gccaacgcat attctgatca ttccgaatat cctcattccg actgtgaacg acgtgtcagc
    58501 tgagcatgag caggcgctgg gacgcatgat caccgtagcg gcaaaaattg ctgagcaaga
    58561 aggtattgcc gaagatggct atcgtctgat catgaacacc aaccgccatg gcggacaaga
    58621 ggtttaccac atccatatgc atttgttggg tggccgtccg ctgggaccaa tgctgacgca
    58681 taaaggtctg taacgatgag aaaaggatgc tttgggctga tgtctctggc gttgttagtg
    58741 ctggtgggct gtcgttcaca tccggaaatt ccggtgaatg atgagcaatc gctggtgatg
    58801 gagtcatctt tattggctgc gggcatcagt gcagaaaagc ccgtcctttc gacatctgat
    58861 attcaacctt cagcatcctc aacgctttat aacgaaaggc aagaacccgt taccgttcat
    58921 tatcgttttt actggtatga cgccagaggg ctggagatgc atcctctgga aaggccacgc
    58981 agcgttacca ttcccgcaca ttcggcggtc acgctgtacg gcagcgccaa ttttctgggg
    59041 gcgcacaaag tcagacttta tctatatttg taaggggtga atcttgatga caaaaatgag
    59101 tcgctacgcc ttgattaccg cgctggcgat gtttcttgcc gggtgtgtgg ggcaacgtga
    59161 acctgcaccg gtagaagaag tgaaaccagc gccggaacaa cctgctgagc cacaacagcc
    59221 tgtccccaca gtgccatcgg tgccgacgat accgcagcag ccaggcccaa ttgagcacga
    59281 agatcaaact gcaccgcctg cgccgcatat tcgtcattat gactggaatg gcgcaatgca
    59341 gccgatggtc agtaagatgc ttggggctga cggcgtgact gcgggtagcg tcctgctggt
    59401 tgatagcgtt aacaaccgta ctaacggttc gttgaatgcc gcagaagcga ccgaaacgct
    59461 gcgaaatgcg ctggctaata acggtaaatt taccctggtt tccgcccagc agctgtcaat
    59521 ggctaagcaa caattaggtt tatcgccgca ggacagttta ggcacccgta gtaaagccat
    59581 aggcattgcc cgcaatgttg gcgctcatta cgtgctgtac tccagcgcct ctggcaacgt
    59641 taacgctccg accctacaaa tgcagctgat gctggtgcag acgggcgaaa ttatctggtc
    59701 aggtaaaggt gccgtttcgc agcaataatc ccctcacgcg cgacgaattg ctgtcgcgct
    59761 ttttcccgca gtttcatccc gtcacgacgt ttaatagtgg gcttagtggc gggagttttc
    59821 tcattgaaca tcagggccag cgttttgttg tgcgtcagcc gcacgatcct gatgcgccgc
    59881 agtccgcgtt cttgcgccag tatcgggctt tatcacaact acccgcatgc attgcaccga
    59941 agccgcattt atatctccgt gactggatgg tggtcgacta tctgcccggc gaggtaaaaa
    60001 cgtatttgcc ggataccaac gaactggcag gcttgctgta ttatctacat caacaaccgc
    60061 gttttggctg gcgaataacg ctgttgccgt tactggaact gtactggcag caaagcgatc
    60121 cggcgcggcg gacagtgggt tggctgcgaa tgttaaaacg tctgcgcaaa gcgcgggaac
    60181 cacggccttt acgcttaagt ccattgcata tggatgtcca cgccggaaat ttagtgcata
    60241 gcgcgtcagg gttaaaactc atcgactggg agtatgccgg agatggtgat atcgcgctgg
    60301 aactggcggc ggtgtgggtg gaaaatattg accagcatcg gcaattggtc aatgactatg
    60361 ccactcgcgc gaagatttat ccggcgcaat tatggcgtca ggtcaggcga tggtttccct
    60421 ggctgctgat gctcaaagca gggtggtttg agtaccgctg gcgacaaacc ggcgatcaac
    60481 aatttatcag gctggccgat gacacgtggc ggcagctatt aataaaacaa taaggagagc
    60541 agtgtgggtc cagtaatgtt ggatgtcgaa ggttacgaac tggacgcgga agagcgtgaa
    60601 atactggcgc atccgctggt gggagggctg attctcttta cgcgaaacta tcatgatcct
    60661 gcccagttac gtgaactggt gcgccagatc cgcgcagcat cgcgcaatca tctggtggtg
    60721 gcggtagatc aggaaggtgg acgcgtgcag cgttttcgtg aaggttttac ccgcttgcca
    60781 gcggcacaat cattcgctgc gctgttggga atggaagaag gcggaaaact ggcgcaagag
    60841 gcaggttggt tgatggccag tgaaatgatt gctatggata ttgatatcag ctttgcgcca
    60901 gtgctggacg tcgggcatat cagcgcggcg attggcgagc gttcttatca tgccgatcca
    60961 caaaaagccc tggcaattgc cagccgtttt attgatggta tgcatgaagc gggaatgaaa
    61021 actaccggga aacacttccc aggtcacggt gcagtaacgg cagactcaca caaagaaaca
    61081 ccgtgtgacc cacgtccgca agcggagatt cgcgctaaag atatgtcggt cttcagttcc
    61141 ttaatccgcg aaaataaact cgacgccatt atgcctgcgc atgtgatcta cagtgatgtt
    61201 gatccgcgtc cggcgagcgg ttctccctac tggctgaaaa ccgttttgcg tcaggaattg
    61261 ggttttgacg gcgtgatttt ctctgacgat ttatcgatgg aaggcgcagc gattatgggc
    61321 agttatgccg agcgtggaca ggcatcactg gatgcaggtt gcgatatgat cctggtctgc
    61381 aataatcgta aaggggccgt cagcgtgtta gataatctgt caccgatcaa ggcagaacgt
    61441 gttacacgtt tgtatcataa aggttcattt tcgcgacagg aactgatgga ctcggctcgc
    61501 tggaaagcga tcagcacccg tctgaatcag ttacatgaac gctggcagga agagaaggca
    61561 ggtcattaac cctggcttat gtgaggaagc gatgattatc tatttgcacg gttttgactc
    61621 taacagtccg ggtaaccacg agaaagtctt acaattgcag tttattgacc cggatgtacg
    61681 gctaataagc tacagtacgc ggcatccgaa acatgatatg cagcatttgc tcaaagaagt
    61741 ggacaaaatg ttgcaactga acgtcgacga gcgcccgctc atttgtggtg ttggtctggg
    61801 cggttattgg gctgagcgga ttggttttct ctgtgatatc cgccaggtga tcttcaaccc
    61861 caatttgttc ccttatgaga acatggaagg gaagattgat cgcccggaag agtatgccga
    61921 tattgccact aagtgtgtga ccaacttccg tgagaagaat cgcgatcgtt gcctggtgat
    61981 tttgtcgcgt aatgatgaag cgcttaacag ccagcggaca tctgaagagt tgcatcatta
    62041 ttacgagatt gtctgggacg aagagcagac gcacaaattc aagaatatct ccccgcattt
    62101 acagcgcatt aaagcgttca aaaccctcgg gtaaatgccc tcgtcgcatc aggtaacctt
    62161 gccggtacct gatgcgctcc ggattctgtg ggttggataa ggcgttccgc cgcatccgac
    62221 agtcgagcat caatgcctga tgcgcctctt atcaggccta ccgaacgccc tccatacaca
    62281 cctcactcta tatcattctc acaaatcagc tcaaataata aacaacaaac tctgtttttt
    62341 gatctcaccc ggtaaagtcg cctatctttt cagcaataaa acttgattaa catcaatttt
    62401 ggtatgacca atgcaccatt catgttattc tcaatagcga agaacatttt cattgctgta
    62461 acctgttgtt aattaagagc tatgttaata accattaatt aacaattggt taataaattt
    62521 aagggggtca cgttgactac gccattgaaa aaaattgtga ttgtcggcgg cggtgctggt
    62581 gggctggaaa tggcaacaca gctggggcat aagctgggac gcaagaaaaa agccaaaatt
    62641 acgctggtcg atcgtaacca cagccacctg tggaaaccgc tgctgcacga agtggcgact
    62701 ggctcgcttg atgaaggcgt cgatgcgttg agctatctgg cccatgcgcg caatcatggt
    62761 ttccagttcc agctgggttc cgtaattgat attgatcgtg aagcgaaaac aatcactatt
    62821 gcagaactgc gcgacgagaa aggtgaactg ctggttccgg aacgtaaaat cgcctatgac
    62881 accctggtaa tggcgctggg tagcacctct aacgatttca atacaccagg tgtcaaagag
    62941 aactgcattt tcctcgataa cccgcaccag gcgcgtcgct tccaccagga gatgctgaat
    63001 ctgttcctga aatactccgc caacctgggc gcgaatggca aagtaaacat tgcgattgtc
    63061 ggcggcggcg cgacgggtgt agaactctcc gctgaattgc acaacgcggt caagcaactg
    63121 cacagttacg gttataaagg tctgaccaac gaagccctga acgtaacgct ggtagaagcg
    63181 ggcgaacgta ttttgcctgc attgccgcca cgtatctctg ctgcggccca tagtgagtta
    63241 acgaaactag gcgttcgcgt gctgacgcaa accatggtca ccagtgctga tgaaggcggc
    63301 ctgcacacta aagatggcga atatattgag gctgatctga tggtgtgggc agccgggatc
    63361 aaagcgccag acttcctgaa agatatcggt ggtcttgaaa ccaaccgtat caaccagctg
    63421 gtggtggaac cgacgctgca aaccactcgc gatccagaca tatacgctat tggcgactgc
    63481 gcgtcatgcc cgcgtccgga agggggcttt gttccgccgc gcgctcaggc cgcacatcag
    63541 atggcaacct gcgcaatgaa caacattctg gcgcagatga acggtaagcc gctgaaaaat
    63601 tatcagtata aagatcacgg ttcgctggta tcgctgtcga acttttccac agtcggtagc
    63661 ctgatgggta acctgacgcg cggctcaatg atgattgaag gacgaattgc gcgctttgta
    63721 tacatctcgc tgtaccgaat gcaccagatt gcgctgcatg gttactttaa aaccggatta
    63781 atgatgctgg tggggagtat taaccgcgtt atccgtccac gtttgaagtt gcattaatcg
    63841 gcttgtactg gcggatgcgg cgtaaacgcc ttatccgccc tggacaaatg gtacgagcgg
    63901 taggcctgat aagacgcgtt aagcgtcgca tcaggcattc cttcagactc ctccgaatcc
    63961 ctaagtattt ccagccattc ccgcgctttt catcttctgt ctgatagctg cttttctcct
    64021 tcgcttgcat gattggcata acagcaaaga aggaggtatt cccgtgaata agtcaatgtt
    64081 ggcgggtatc gggattggtg tcgcagctgc gctgggcgta gcggcagtgg ccagtctgaa
    64141 cgtgtttgaa cgtggcccgc aatacgctca ggttgtttct gcaaccccaa tcaaggaaac
    64201 ggttaaaaca ccgcgtcagg agtgtcgcaa cgttacagtg acccatcgtc gaccggtgca
    64261 ggatgaaaat cgcattaccg ggtcggtgct cggcgctgtt gctggcggcg tgattgggca
    64321 tcagtttggt ggtggtcgcg gtaaagatgt cgccactgtt gtgggggcgc tggggggtgg
    64381 atatgcaggt aaccagatcc agggctctct ccaggaaagc gatacttaca cgactacgca
    64441 acagcgttgt aaaacggtgt atgacaagtc agaaaaaatg ctcggttatg atgtgaccta
    64501 taagattggc gatcagcagg gcaaaatccg aatggaccgc gatccgggta cgcagatccc
    64561 gctagatagc aacgggcagc tgattttgaa taacaaagca taacaaggtt gtactctgca
    64621 atttagcccc tcattcgctc aagctgaggg gctttttttg cgacctattt cagcagttcc
    64681 ggccataaac gcaaagtcgt tcgggcaatc tgcatcagtt tttccagcga tgcaccttcg
    64741 cgtgcgctga tcgacatccc ctgaataata caattaagga attctgccag atgcgtcacg
    64801 tcacagtggg tcgggatttc cccgcgcgct tgtcgttgac ataaaaactg ctgcaaagtg
    64861 cgctcttgca tcgcatggcg tgatttcagc gtattggcga tatctccaga atccggggag
    64921 agggtggtgc agttgttgat catgaagcag cccgccgggg tgtctttgct ggtaaagcag
    64981 ttggcgatgg cagcaaaata gtcagccagc gcagacgcca cgcttttctc ttcacaaaac
    65041 agctgtgctt catgcttagc ggcaaaacga tcgatatagc ggtcgagtac ggcgcggaat
    65101 aacccctctt tgttggtaaa ttccgcgtat agcgtgggcg ctttagctcc ggtcgcttca
    65161 acgaggtccg caagagaagt cgcttcataa ccgtgctgcc agaacaattt catggcctta
    65221 tcaagcgcgg catccctgtc gaacactttt gggcggccac ggcttttttt tacacattgt
    65281 gttgagtcag ttgccatgat gccgttgtac ctggtgactg tgaatgaaag gttattataa
    65341 aaataatcac ctccgttcac cagtccagat tccataaaaa taattgcttt ctatttaact
    65401 gaaatttaaa ggtttttaaa ttaattaatg attgttataa aaatatcttg tatgtgatcc
    65461 agatcacatc tatcatttag ttatcgatcg ttaagtaatt gcttgcgacg tcattcatct
    65521 gcataaggcc actattatga aaaacgtaaa aaccctcatc gctgcggcga ttttaagctc
    65581 catgtcattt gccagctttg cggctgtcga agttcagtca acgccagaag gccaacaaaa
    65641 agtcggtact atcagtgcta acgcggggac aaatctggga tcgctggaag agcagctggc
    65701 gcaaaaagcg gatgagatgg gcgcaaaatc tttccgtatt acttctgtaa ccggtccgaa
    65761 taccctccat ggaacagcag taatttataa ataagcatta accctcatta atgcctgcta
    65821 ctgcttgttt ttttccccgc gacatgccgt gtcgcgggga tttttttatc cgagctttac
    65881 agagtttgtg ggctcacttg atgacggcgg acatccaccg gcatcccgga acggacatcc
    65941 atcacatgtt gcatcacttc agcgtcagtt tgtgctgcat ctttaaatga ttgcattgcg
    66001 ctattcagcg taattggcag cagctgcgga tcgtcatcaa tcttctctga cagcggctga
    66061 tgtacttcaa ccagacgcgc accgtttggt tcggcagaga ctttaatcgg ggtgttgatg
    66121 atattcactt tggtgcctgg ggtgacctgg ctaaagagtg ttttgatatc gtcatcccgc
    66181 agacgaatac agccagaact tacccgcatg ccaatgccga aatcagcgtt cgtaccgtga
    66241 agcaaataaa cgccgccata ggccgccaga cgaatcgcat gatggcccat tgggttatcc
    66301 ggtccagccg gaactaccgc aggcaactca attccctgtg ccttataccg cgcgcgaatg
    66361 tttgccgttg gcgtccaggt tgggtttgca cgcttgtcag aaacggtggt caccattgtc
    66421 ggtgtcagcg tgtcaccacc taactgacca atgccgatgg gatatacggt taccgaattt
    66481 tttcccggcg gataataata gagacgcagc tccgcaatgt tgatcacaat gccttcgcgc
    66541 ggcgcgtctg gaagtagggt ttgcaacggg atcgttaaca cgctacccgc gcgcggtacg
    66601 taaggatcaa cgccggggtt agcctgtaac agagcgagaa agccgacgtt atattttttg
    66661 gcgatcgctt ccagagaacc accgtcattt tccaccacat gaaatttgtt ttcaccaacc
    66721 agacgactgc ccgctgacgg cagcggccag gtgttggctt ttgccggtag cgccagcgcc
    66781 acggcaacgg cgaacgtaaa aaacgttagc cagcgagaaa aacgcgtttt gatcatcacc
    66841 aaagtccata ataaatataa ggttattgta ataaaaaggt aagtctgaat tatggagggt
    66901 gacagtgtcg gataatgcag ggaagtgcaa agaatttgta aatattgcag atgggggcgc
    66961 agaaacgccc ccgatttacc attaagcgat cgcgttctct tccagttcac gcataaactg
    67021 gcgtacccat tcgatacgcg ttttccgctc gctcaaatcc tgaataaact tcagacgcgt
    67081 cggaccatca aggcggtaat gctgcggctg tttttgcagc aaaccaatca accaggccgg
    67141 attaacgtga ttcttctcgg caaattcgat cacgccgcct ttctcattac cttccagctt
    67201 cctgatcccc agtttctgcg cttgctggcg cagacgggca atatccagca gggtacgcgc
    67261 cggatccggc agcaggccga agcgatcgat aagctcgact ttgatctctt ccagttcgtt
    67321 ttccgttttg gcgctggcaa tacgtttgta gaacgacaga cgcgtgttca cgtcagggat
    67381 gaaatcatct ggcaatagcg acggcatccg cagctcgact tctgtttgct ggctggtgag
    67441 atcttccagc gacggctcgc gtccggcttt cagcgcatcg acggcgtttt ccagcaactc
    67501 catatacagc gagaaaccga tggtttccat tgagccgctt tgttcttcgc caagcagttc
    67561 acctgcgccg cggatctcca gatcgtgcgt tgccagcgca aaacctgcac cgagatcttc
    67621 cagcgaggca atcgcttcaa gacgtttttg cgcatcggta gtcatcgctt ttggatgcgg
    67681 cgtcagtagc catgcatatg cctggtggtg agaacgcccg acgcgaccgc gcaactggtg
    67741 caactgtgcc agaccgaagt gatccgcgcg ttcaatgata atggtgttgg cggtaggaat
    67801 gtcgatcccg gtttcgataa tggtggtaca aaccagcacg ttgaaacgtt gatgatggaa
    67861 atcattcatc acccgttcca gttcgcgctc acgcatttgc ccgtgaccga tagcaatccg
    67921 cgcttcaggt accagttctg ccagccgttc ggcggctttc tggatgtttt ccacatcatt
    67981 gtagagataa taaacctgtc ctccgcgcaa aatttcacgc aggatcgcct cccggaccac
    68041 cagactgtca tactcgcgga caaaggtttt aactgccaga cgacgggcgg gcggcgtggc
    68101 gataatcgac agatcgcgca ttccgctcat tgccatattc agcgtacgtg ggatcggcgt
    68161 tgcggtaagc gtcagaatat ccacgttcgc gcgcatcgct ttaatgcgct ctttatgacg
    68221 caccccgaag cggtgttctt catcgacaat cagcaggcct aaatctttaa acttgacgtc
    68281 actttgcagc agtttgtgcg taccgatcag aatatcgatt ttcccttccg ccacttccgc
    68341 aaggatttgc gtctgctctt tggcgctgcg gaaacgggag atcatttcga tacgtaccgg
    68401 ccagttggcg aaacggtcgc ggaagttgtc gtaatgctgc tgcgcgagaa gggtggtagg
    68461 caccagcacc gccacctgtt tgtggttatc taccgccagg aaagcggcgc gcatcgccac
    68521 ttctgtttta ccaaagccaa catcgccgca caccagacga tccattgcca gcggctgaca
    68581 catgtcgcta agcaccgcat taatggcttg agcctgatcc ggcgtggttt caaacgggaa
    68641 gctgtcgcag aataactgat actgctcacg atcgtgttta aacgcgaagc cctctttggc
    68701 ggcgcgttgc gcgtagatat ccagcaattc cgccgccaca tcacgcactt tttccgccgc
    68761 tttctgccgt gcgcgtgacc aggcatcgcc accaagttta tgcagtggtg cgttttcttc
    68821 tgcgccaccc gcgtaacggc taatcagatg cagtgacgac accggaacat acagtttggc
    68881 gtcgttggca taggtgagca tcaaatactc gccagtaatg cctccagctt cgagcgtggt
    68941 cattccggca taacgaccga cgccgtgctc cagatggacc accggctgac caatatgcag
    69001 ctccgcaagg ttacggatca gtgtatcggg gttgatggcg cggcgagaat cctgacgacg
    69061 acgggcaacg cgttcaccga gcagatcgct ttcgcaaatc agcgccaggt tacgcatcgt
    69121 atcgacaaaa ccatgttcgg cagcgccaat catcagataa cgcccacggt tgctggcttc
    69181 atcaagacgc ataatgcgtt gcggagcaat tttaattcgc gcgagcagtt cacccagcgc
    69241 ttcacggcga ccttcacttt ctaccgagaa caccaccgga ccgtcgaaag tctcgaggaa
    69301 cttacgcagc gcatccagcg gcgctttttg ttgtgcctga atggccaggt ctggcagttt
    69361 ctggaaacct aaattggcat tcgctgcttt tgtcggtaaa tgttcagttt ttagctgcac
    69421 acgcggccag tttttcagct ctgaaaagag ctcgtctacc cgcagccaga gcgattgtgg
    69481 tggcaacagt gggcgcattg ggtcgacacc gcgattctca aaacgcgcga gcgtgtcagc
    69541 ctggaaacgt tcggcactgt tttccagatc gccagtattt atcagcagcg tattggcagg
    69601 gaaataactg aacagtggcg gcagcggttc gctgaagaat agcggctgcc agtactcgat
    69661 cccggcaggt aatgtgcctt tactcacttg ctggtaaata tgttccggat cgcgcttcac
    69721 ttcgaaggta tcgcgccact ggctgcggaa cagttcaatt gccgctttat cagtcggaaa
    69781 ttcgtgcgcg ggcagcagat tgatcgcttc tacttcctcc agcgtgcgct ggctatcgac
    69841 gtcaaacacc cgcaggctgt cgatttcatc atcaaagaaa tcaagacgat aaggcagctc
    69901 actccccatc gggaagagat ccagcaacgc gccgcgcgtg gcgtattcgc cgtgctccat
    69961 cacctggtca acatggcgat aaccggcgct gtccagttgg gttcgtaatg catctcgtga
    70021 caggcgctga ccttttttca tcaccagcgc atgaccgtgg agaaagctgt gcgggcaaac
    70081 gcgctgcata agcgtattca ccggaacaat cagtacgcca cgctgcatgg tcggtagctg
    70141 gtaaagggtg gaaagacgcg aggagataat gtcctgatga ggcgaaaaac tgtcgtaagg
    70201 gagggtttcc cagtccgcca gattcatcac catctgatcg gtgaactggc tgatttcatc
    70261 atgcaaacgc agagcatttt gcatatctgg tgcaatgagt accaccggac cggcgtgacg
    70321 ttcggcaatt tccgctacca gcgtcgcgca ggcagcgccg gttaactcgc ccagcagacg
    70381 ctgctcaccc gctttgacgg gcagcgtata acgatattgt tcaggcataa cggttgtcag
    70441 attctcgtta ggatatgcct caacatatga gggcatatct ctgataagca atgctgttca
    70501 ttatccgtaa tcggcatggt ttagcaaatt gaatcgcccc ggctggggcg atggcttagc
    70561 gggagtaact gacggtagcg ggcagagaag gcggggagaa caacagatcg ctgaatgcgc
    70621 gctgtgaaag ttttcttacc agcaagcccg caacagtaca aatcaacaaa ctgacaaagg
    70681 ggtaaatcag caggagcgtc agttcgactt gcggcgacca gcgtaccgcg ttcatttgcg
    70741 gaatcaggct taagctgaat atttctacca gaatgcgatg ggtggtatag atagcaatgg
    70801 tgttggagcc aatcacattc agcaggctgg aggaacgcat accgaaacgc tgctcgtact
    70861 gataaaacag cttcatgatt accacaatcg ataccagcga caacagcagc gagatattaa
    70921 acaaccaggc accgaccgcc agaacggtca gcaaagaagc catcagcaga tggcggcgca
    70981 acggtaccgc tttaacacag gtcattaatg ttgtgccaaa ccatgcgcca aggctgtaat
    71041 aaggcaaatt gcggatcaca ctgttcattc ccaaccacgg tgtgggtacg aaattaacag
    71101 ccacactcat cagtacaaac agaacaaata acggcagggc caggcggtta aaaattttac
    71161 atatcacgaa atagacaatt aacgcataca gataccacaa gctggtgctg gcggtgatca
    71221 tcccgtgcag gaactcaccg gtggaatcgg cataagcggc attggaggca ttgcttaaat
    71281 cgcgctcagg tgccagccac tggttcagcg cacttaacgc cagccactgc accacgcccc
    71341 aaagcgccag cacccagaag atgttccaga tgcgtttatc gagacaattt ccccacggta
    71401 cgctgtcgat atagcggcga atcaaatagc cagagataaa gaaaaaaacc ggcatacgaa
    71461 agggtgcaag gtaaagattg aaatagatcc agcatttgct caggacttct gataacggat
    71521 gctggaaagt ggtcagatgc ggataaaagg taatgaccga gtgataaatc accaccagac
    71581 aaatacataa ccctttgatc tggttaatcc atagctcttt ttgtttcatc aggacaacac
    71641 gctcttttta tttaacatgc ggttgatcct gcggggcgag tcgcgtggtg taatgcgtat
    71701 ctgtctgtat ttggttattt ttcagataaa gatcaggcca catggccgat gataaaatga
    71761 ggcactttag ccgattgatt tgccggtact tttcagatta gccctgacga tcacttacag
    71821 ttcagacgtt tacccatcct gctttcgctt atatactcgt gtctttgcta cagcaaccag
    71881 acggatttca tgtaccaacc tgtcgctcta tttattggcc tgcgttacat gcgtgggcgt
    71941 gcagcggatc gcttcggtcg tttcgtctcc tggctttcta ccatcggcat taccctcggg
    72001 gtgatggcgc tggtcacagt attgtcagtg atgaacggct ttgagcgcga gctgcaaaac
    72061 aacatccttg gcctgatgcc acaggcaatt ctctcttctg agcatggctc tcttaacccg
    72121 cagcaactcc cggaaacggc cgtcaaactg gacggcgtta atcgcgtcgc acctattact
    72181 accggtgatg tggtactgca aagcgcgcgc agcgtggcgg tcggggtgat gctgggtatc
    72241 gacccggcgc aaaaagatcc actaacgccg tatctggtca atgtgaaaca aactgacctc
    72301 gagccgggga aatataatgt catcctcggt gaacagcttg cctcacagtt gggcgtcaat
    72361 cgcggtgatc aaatccgcgt gatggtgcca tctgctagcc agttcacgcc gatggggcgt
    72421 attccaagtc agcgcctgtt caatgtgatt ggcactttcg ccgccaacag tgaagtcgat
    72481 ggctatgaaa tgctggtgaa tattgaggat gcctcgcgtc tgatgcgtta tccggcaggc
    72541 aatattaccg gctggcgttt gtggctggat gagccgctga aagtcgactc attaagtcag
    72601 caaaaactgc ctgaaggcag caaatggcag gactggcgtg accgtaaagg cgagctgttc
    72661 caggccgtac gcatggaaaa aaatatgatg ggcttgctgc tgagcctgat tgtcgccgtt
    72721 gcggcgttta acattattac ctcgctggga ctgatggtga tggagaagca gggcgaagtg
    72781 gccattcttc agactcaggg gctgactccg cgacaaatca tgatggtctt tatggtgcaa
    72841 ggggccagcg ctgggattat cggtgcgatc ctcggagcgg cgcttggcgc actgcttgcc
    72901 agccagttga ataatctgat gccgataatc ggcgtcctgc ttgatggcgc ggcgctgccg
    72961 gtcgctatcg agcctttaca ggtcattgtt attgcgctgg tggcgatggc tatcgcgctg
    73021 ctgtctacgc tttacccttc ctggcgcgct gccgccactc aacccgctga ggctttacgt
    73081 tatgaataag atcctgttgc aatgcgacaa cctgtgcaaa cgctatcagg aaggcagtgt
    73141 gcaaaccgat gtactgcaca atgtcagttt cagggtgggc gaaggtgaaa tgatggcgat
    73201 cgtcggcagc tctggttccg gtaaaagcac cttgctgcac ctgttaggcg ggctggatac
    73261 gccaacctcc ggcgatgtga tctttaacgg tcaaccgatg agcaaactgt cttcggcggc
    73321 gaaagcagaa ctgcgcaacc agaagctggg atttatttat cagtttcacc acctgctgcc
    73381 ggattttacc gccctggaaa acgtggctat gccgctgttg attggcaaga aaaagcccgc
    73441 tgaaatcaac agccgtgcgc tggagatgtt aaaagcggtg gggctggagc atcgtgcgaa
    73501 tcaccgccca tctgaacttt ctggcggcga acgccagcgt gtggcgattg cccgtgcgct
    73561 ggtcaataac ccgcggctgg tactggcgga tgaacctact ggtaacctcg atgcacgtaa
    73621 cgccgacagc atcttccagt tgcttgggga attgaatcgc ttgcagggca ccgccttcct
    73681 ggtggttact cacgacctgc aactggcgaa acgtatgagc cgccaactgg aaatgcgtga
    73741 tggacgtctg acggcggaac tgagcctgat gggggcggag taatggcgat gcctttatcg
    73801 ttattgattg gcctgcgttt tagccgcgga cggcgacgcg gcggcatggt gtcgttgatc
    73861 tccgtcattt ctaccattgg cattgccctg ggcgtggcgg tattgatcgt cggcttaagc
    73921 gcgatgaacg gctttgaacg cgaactgaat aaccgcattc tggcggtagt accgcacggt
    73981 gaaattgagg cggtagatca gccgtggacc aactggcagg aagcactgga taacgtgcag
    74041 aaagtgccgg gcattgccgc cgctgcgccg tatatcaatt tcaccgggct ggtggagagc
    74101 ggcgcgaatt tgcgcgcaat ccaggtgaag ggcgttaacc cgcaacagga acagcgtctg
    74161 agcgcattac cctcgtttgt tcagggggat gcctggcgca attttaaagc gggcgaacag
    74221 caaattatca tcggcaaagg cgtggcggat gcgctgaaag tgaagcaggg cgattgggtg
    74281 tcgattatga tccccaactc gaatcccgag cataaactga tgcagccaaa acgtgtgcgt
    74341 ttgcacattg ccggtatttt gcagttgagt ggtcaactcg atcacagttt tgccatgatc
    74401 ccgctggccg atgctcagca atatcttgat atgggttcca gcgtgtcagg tattgccctt
    74461 aaaatgacgg atgttttcaa cgccaataag ctggtacgcg atgcgggtga agtgaccgac
    74521 agctatgttt atattaaaag ctggattggt acttacggct atatgtatcg cgatatccag
    74581 atgatccgcg ccattatgta tctggcgatg gtgctggtga ttggcgtggc ctgtttcaac
    74641 atcgtctcca ccttagtgat ggcggtgaaa gacaagagtg gcgatatcgc agtattaaga
    74701 acgctggggg cgaaagatgg tttaattcgc gccatctttg tctggtatgg attgctggca
    74761 gggctgttcg gcagcctgtg tggtgtggtt atcggcgtgg tggtttcact taaacttacc
    74821 ccgattattg agtggattga aaagctgatc ggtcatcagt tcctctccag cgatatctat
    74881 tttattgact tcctgccatc ggaattgcac tggctggacg tcttctacgt actggtcaca
    74941 gcattgttgc tgagtctttt ggcaagttgg tatccggcgc ggcgcgccag taatattgac
    75001 cctgcgcgag tccttagcgg ccagtaaagg cagtacatta aaacaaggag cggcaatgta
    75061 ttacgggttt gatattggtg gaacaaaaat tgcgctaggc gtgtttgata gcggtcggca
    75121 gttgcagtgg gaaaagcggg tgccgacacc gcgtgacagc tatgacgcat ttttagatgc
    75181 agtgtgcgag ctggtagccg aagctgaccg gcgttttggc tgtaaaggtt ctgtcggcat
    75241 cggtattccg ggaatgccgg aaacagaaga tggtacgctg tatgccgcca atgtccctgc
    75301 tgccagcggt aaaccgctgc gtgccgacct gagcgcacgt cttgatcgcg atgtacgcct
    75361 tgataacgat gccaactgct ttgccctttc tgaagcatgg gatgatgaat ttactcaata
    75421 tccactggtg atggggttga ttctcggcac cggcgttggc ggcgggctga ttttcaacgg
    75481 taaaccaatt accggtaaaa gctatattac cggcgagttt ggccatatgc gtctgccggt
    75541 tgatgcgtta accatgatgg ggctggattt cccgttacgc cgctgcggct gtggtcagca
    75601 tggctgcatt gaaaattatc tgtctggtcg cggttttgcg tggctgtatc aacactatta
    75661 tcatcaaccg ttgcaggctc ctgagatcat tgcgctttat gatcaaggcg atgagcaggc
    75721 aagggcgcac gttgagcgtt atctggattt attagcggtt tgtctgggaa atatcctgac
    75781 cattgttgac cctgacctgg tcgtcattgg tggtggctta tcgaatttcc cggcaatcac
    75841 aacgcaactg gcggaaaggt tgcctcgtca tctcttacct gtagctcgtg ttccgcgcat
    75901 tgaacgcgcg cggcacggtg atgcgggggg aatgcgtggt gcggccttcc tacatctaac
    75961 cgattaaaca acagaggttg ctatgctgtc gcgtcggggt catcggttaa gtcgttttcg
    76021 taaaaataaa cgtcgcctgc gcgagcgttt gcgtcagcgt atttttttca gagataaagt
    76081 ggtgccggaa gcaatggaaa aaccaagagt actcgtactg acaggggcag ggatttctgc
    76141 ggaatcaggt attcgtacct ttcgcgccgc agatggcctg tgggaagaac atcgggttga
    76201 agatgtggca acgccggaag gttttgatcg cgatcctgaa ctggtgcaaa cgttttataa
    76261 cgcccgtcgt cgacagctgc agcagccaga aattcagcct aacgctgcac atcttgcgct
    76321 ggctaaactg caagatgctc tcggcgatcg ctttttgctg gtgacgcaga atatcgacaa
    76381 tctgcatgaa cgcgcaggta ataccaatgt cattcatatg catggggaac tgctgaaagt
    76441 acgttgttcg caaagtggcc aggtcctcga ctggaccgga gacgttacgc cagaagataa
    76501 atgccattgt tgccagttcc cggcaccctt gcgcccgcac gtagtgtggt ttggcgaaat
    76561 gccactcggt atggatgaaa tttatatggc gttgtcgatg gccgatattt tcattgccat
    76621 tggtacttcg gggcatgttt atccggcggc tgggtttgtt cacgaagcga aactgcatgg
    76681 cgcgcacacc gtggaactga atcttgaacc gagtcaggtt ggtaatgaat ttgccgagaa
    76741 atattacggt ccggcaagcc aggtggtgcc agagtttgtt gaaaagttgc tgaagggatt
    76801 ataaagcggg aaaaattgcc tgatgcgcta cgcttgtcag gcctgtgaag gggcgcaatt
    76861 aattgaattt gcgggttttg tgggccggat aaggcgttcg cgccgcatcc ggcataatac
    76921 atgcgaactt tactgcctct tttaccgttt actcgtccat agtaacgtgg caataaacgt
    76981 acagacacca atcagccctg cccagagtgg cgatattctg gtaatcgcaa agctgttatc
    77041 gatatacaaa cataaagcct gataccccat attggcaaca gccatcgcca ctgcaaattt
    77101 taacgcataa taaaggcgcg ttgtgtagtt aaaaaatgca gcaaagacga tgaatgcaac
    77161 tgcccaggca aaaaggccta atgtcgtggt actgccgggt ggatcatttt catgctgttg
    77221 tccgggatgt ggataatatg tcagtggtgg cgtcggttgt tttaatatct gtgcttgtaa
    77281 aacagccaat gcattattga taccctgata ccagagatcc gcatgtaaat aagccagcgt
    77341 gtgattttct accagaaggc cgagatgttt atcgtccata ctgctttcga cctggccgtt
    77401 ggttatcata attaaccgtg gctcgcgggt agtaaagagt aacagaacac ttccttttac
    77461 attactcatt gaagatgccg catgcgccag catcgctttg ccattttgcc tgagactggc
    77521 ttcattatta atgtcgggta aaaccacaat aaacggtgca actccggttt gttggcgaaa
    77581 aagttcggct ttatgcgcga tctcttcagc atctggtaca gtaccggtaa catctgtaaa
    77641 aagagtcttc attattggaa ttaagggctt actgattgcc gtcagaggca ggaacatcag
    77701 taaactgagc agtatcatga ttttttccat gggcgggact tccgtttcct gcgtgtcttt
    77761 ttagaggagg gcatactgat taataaaccg acggcgataa aaataacgcc cataatgccg
    77821 ataaaccccc atttaaaaaa attaacccac aggttattta tctcagcctt ttcaggatgt
    77881 cctggagaat aataaacatt aactttatcg ccttcgctgt cttcataaaa atcgctgcca
    77941 atactcgaat tgaaaattag cgtgtaatct ggcgtcggac gaaaggcaac gacgggatac
    78001 cacgaaccat ttttacccgt gcgatgagaa tgactggagt gccagacggt atcaacaatg
    78061 acgccttcag tatgaatcgc gtctttgcta taggtgaact ggttataaaa gataacaaca
    78121 gaaatgagga tcagtaccag accgataccg aagaatatac gaaagaaaac tctactgtcc
    78181 tggctcatta gccacatcct tgctaaataa aaaacgggcg gtaataccac cgcccgcttg
    78241 ctgaattaac gtcctgcttt cagcttctga taatactctt cataaatgct gctggcggag
    78301 cctacgtcat tctgccattc accattttta atggtttcag catccgggta gagcgtttta
    78361 tcgttcgcca cttccggact taacagctta cgcgccgcaa ggtttggcgt tggataaccg
    78421 atagtttcag caacctgttt tgccacatcc gggcgcagca ggaagttgat caatttcagc
    78481 gcgccttctt tgtttttggc atttgccggg atcgccaggc tgtccatcca gaaaatgccg
    78541 ccttctttcg gccataccac atctattggc gtacccgcct ggcgtgcaac gaaagcagaa
    78601 ccgttccaga tcatgccgag gttaacttcg ccttccatat acgggttagc ggggttatcg
    78661 gagttaaacg ccgcgacgtt tggcatcagt tttttcagct cgttgtatgc agcttcaatc
    78721 tctttcggat cggtggtgtt accggagtag cccagcttac gcagcgccat ctggaacact
    78781 tcacgggcat cgtcggtcag cagcaggctg cctttgtact caggcttcca cagatcggcc
    78841 cagctggtga cagatttcgg atccaccgca tcaccgttaa cgccaatcgc cgttgcaccc
    78901 cagatatacg gaatggaata gtcgttattc gggtcaaacg gcttgttgag catgtctgga
    78961 tcgaggttgc tgaaattgct taacttcgac ttgtcgatct tctggatcat cccttcttta
    79021 cgcattttat cgacgtaata ggttgaagga accaccaggt cataggcacc gtctttgtac
    79081 gttttcagct tcgcgtacat ggtttcgttc gactcgtaag tcgaatagat aaccttaata
    79141 ccggtttctt tggtgaactg ttcaagcagt cctggcggca cgtactcggt ccagttgtag
    79201 aaatacagcg tgttgttgtc atcggcgtga gcggcgctca tgcccagtgc cagagcaccc
    79261 gccgcgagca ggtggcgtga ccattttttc atttaacgtc ccctgtgtta cctttcgttt
    79321 tatcacgagc aataagctgg ctggcaataa ccatcaccag cgacagcacc agtaatatgg
    79381 ttgccagcgc gttaacttcc ggcgatacgc cgactttgac catcgaataa atttttaacg
    79441 gcagaatttc atagcttggt ccggtgacaa acgacgaaac taccacatcg tccatcgaca
    79501 gggtaaagct tagcacccag cccgccgcca ccgctggcat tgccagtggc aggatgattt
    79561 ttcgcagaat gataaattca ctggcaccga gatctttcgc cgcttccagc attcgcacgt
    79621 caaaaccttt caggcgcgaa tacaccgtca ccaccacaaa cggcaggcag aaggtgatat
    79681 gcgagaacag cagcgaccag aagccaagct gaatacccag cagcataaac agcaccagca
    79741 gcgaaatcgc catcacgata tctggtgaca tcatcaccac aaacagcatt ccgctaacga
    79801 acggcttacc acgaaagcga taacggtaca gcgcaactgc cgtcagtgaa ccgataagcg
    79861 tagcaaacgt cgccgaaaac accgccattg ttagtgaatg ctgcgctgcc tgtaacaggc
    79921 tgtcgttgtt catcagcagg ctataccatt tggtggtaaa accctgccag ttgatgccaa
    79981 agcgcgagct gttaaaggag ttcacaatca aaataatgat tgggatatac aggtacgcgt
    80041 agatagcggt cataaaaccg ccgcgaagca gtcgaccgat cattcgagtt ccaccttctt
    80101 attcagcaga cgagaagcgc gccagtaaac cagcagcatc aggcccatta cgatagtcag
    80161 cgtaatgctg gtggctgcac caaacggcca gtcacgaata ttgaggaact gtaccttgat
    80221 gacgttaccg atcagcaggt ttttcgcacc gcccatcagg tcggacacat agaacaggcc
    80281 catcgctggc agcatcacca gcagacatcc ggcaataatt cccggcatcg tcagtgggat
    80341 aatgatgcgg ataaaggtct gcaacttgct ggcaccgaga tcgcgcgccg cctcaagcaa
    80401 cggcttatcc agtttttcga tactggagta cagcggcatc accataaacg gcagcagaat
    80461 gtaaaccaga ccgataatca ccgcactagg cgtgaacatg atgcgaattg gtgtatcgat
    80521 aacgcccagc cagagcaaaa actcgttgag atagcctttg gtgctgagga aaattttcag
    80581 cccgtaaata cgaatcaatg agttggtcca gaacggaaca atcagcagaa acagcagcag
    80641 cggacgcacc ttgtgtggca gcttcgccag aaaccaggca aaaggatagc ccagcactag
    80701 gcaggcgagg gtggcgatca gcgccatatt cagcgagtgc aatagcactt caaaatagag
    80761 cggatcgagc agacgcgtgt agttatccag cgtaaagacc attttgacga aactggcgtc
    80821 gtcgcgggtc aaaaagctgg tgccaatgat catcaggttg ggcagaaaga caaacaacac
    80881 aagccaaccg acaatagtga caatcactac attctggaac ttacttgtgt tcttcatcag
    80941 ccagtacgac ctcccagctt tctacccaat taatggccat tttttggtcg agagagtggt
    81001 caaagtcagg atcgtcttca ttgaagaatt cgctgaccat caccatcttg ccattttcca
    81061 gctcaacaac cgactccagc gtcatgcctt tgtagttacg ctcgcgaacg taaccaatca
    81121 gcccttcggc gtggttgtcg tcgttaatct cttcaacacg taagtcttcc gggcgcagca
    81181 gaacatgcag tttttgcccc ggttcaacgg cgaagttaac gtagatatta cattcgcggc
    81241 cttcaacgtt ggcgcgtacg cgctgctcgt cgagacgttc gatgacagtg gcgttaaaca
    81301 tattgatttc gccaataaac ccggcaacaa acaggttttt cggctcttcg tagatttcac
    81361 gcggcgtgcc gtcttgctca atgcgaccat cgcgcatcac cacgatcctg tccgacatgg
    81421 taagtgcttc ttcctggtcg tgagttacga acacgaatgt aatgccaagc ttacgctgta
    81481 acgctttcag ctcgttctgc atttgcttgc gcagtttgta atccagcgct gagagcgact
    81541 catccagcaa caacagacga ggcttgttaa ccaccgcgcg agcaatggcg acgcgttgct
    81601 gttgaccacc agagagctga tgcggtttgc gttgagcgaa ggtttccagc tgcaccatcc
    81661 gcagggcttc catcacgcgg ggcgtaattt cagcagcggg ggttttttgc atgcgcaacc
    81721 caaaggccac attttcgaac acggtcatgt gggggaaaag tgcgtagctt tggaaaacag
    81781 tgttcacata gcggttttcc gccggaacgt gggtgatgtc ctcgttatcc agcatgatgc
    81841 gtccggaatc aacagtttcc agacctgcaa tcaggcgaag aacggttgtt ttaccgcagc
    81901 cagaagggcc aagcagcgtg aggaactcgc cattgttgat agtcagatcc agctggggaa
    81961 tgacctcttt accatcaaag catttgcgaa ttcccgccaa ttgcaccagc ggtgaaagcg
    82021 aactcggttg tttattcaat tttttactct gtcccatgta aacgcaacgg atggcttacc
    82081 gatgcggggt ttgtggttaa ccaccttggt gactcttaat gagggcggta attctacggc
    82141 aaaccgcttg aatcgccaat ctttgttgtg aattactggc ttagctttat attcattaag
    82201 gtaatgctga taaatattcc cgcttgcagg gctaaaagtg acctgacgca atatttgtct
    82261 tttcttgctt cttaataatg ttgtcacaaa aagtgagggt gactacatgg ataaactact
    82321 tgagcgattt ttgaactacg tgtctctgga tacccaatca aaggcagggg tgagacaggt
    82381 tcccagcacg gaaggccaat ggaagttatt gcatctgctg aaagagcagc tcgaagagat
    82441 ggggcttatc aatgtgacct taagtgagaa gggcactttg atggcgacgt taccggctaa
    82501 cgtccctggc gatatcccgg cgattggctt tatttctcat gtggatacct caccggattg
    82561 cagcggcaaa aatgtgaatc cgcaaattgt tgaaaactat cgcggtggcg atattgcgct
    82621 gggtatcggc gatgaagttt tatcaccggt tatgttcccg gtgctgcatc agctactggg
    82681 tcagacgctg attaccaccg atggtaaaac cttgttaggt gccgatgaca aagcaggtat
    82741 tgcagaaatc atgaccgcgc tggcggtatt acaacaaaaa aacattccgc atggtgatat
    82801 tcgcgtcgcc tttaccccgg atgaagaagt gggcaaaggg gcgaaacatt ttgatgttga
    82861 tgccttcgat gcccgctggg cttacaccgt tgatggtggt ggcgtaggtg aactggagtt
    82921 tgaaaacttc aacgccgcgt cggtcaatat caaaattgtc ggtaacaatg ttcacccggg
    82981 cacggcgaaa ggagtgatgg taaatgcgct gtcgctggcg gcacgtattc atgcggaagt
    83041 tccggcggat gaaagcccgg aaatgacaga aggctatgaa ggtttctatc acctggcgaa
    83101 catgaaaggc accgttgaac gggccgatat gcactacatc atccgtgatt tcgaccgtaa
    83161 acagtttgaa gcgcgtaaac gtaaaatgat ggagatcgcc aaaaaagtgg gcaaagggtt
    83221 acatcctgat tgctacattg aactggtgat tgaagacagt tactacaata tgcgcgagaa
    83281 agtggttgag catccgcata ttctcgatat cgcccagcag gcgatgcgtg actgcgatat
    83341 tgaaccggaa ctgaaaccga tccgcggcgg taccgacggc gcgcagttgt cgtttatggg
    83401 attaccgtgc ccgaacctgt tcactggcgg ttacaactat catggtaagc atgagtttgt
    83461 gactctggaa ggtatggaaa aagcggtgca ggtgatcgtc cgtattgccg agttgacggc
    83521 gcaacggaag taagcgaaaa gggatgcggc atgtgatgcc gcatccggct taaatccaaa
    83581 tttacccttc gaagaaccag tatccgctat tgaccagcgc cgcgagcatc gcgaggaatg
    83641 acggatcttc cagcgcatcg ccaaaattct ccgcggtcag cgcaatgttg ctggcgagcg
    83701 catccagtgc cggacggtgc ggggaatcga tcttctcacc attggcatac acgtcgtcgc
    83761 caatgcgcaa tacgcgcaga ccccccaggc gcaccagcac atcaccttgt ttcagcgcat
    83821 cgtagatttc atccggctga taaggcggct ctggcggcgc gatatccagt tcatgacgtg
    83881 actgggatat aaactcgcca aaccattgtt taaagtgttc cggctggttg atcaattcga
    83941 gcatcatctc acgcagttta tccatctctt gcggcagaac gtccgcagga tgagcgcgag
    84001 gtggaacatc aggatcgctg tagtagttgc cgcccagttc acgttgcagc acataatcgg
    84061 caaatccgct aatcagttcc cgcgtatttg gcgcgcgaaa acccacggaa tagttcatcg
    84121 cattttccag cgcgtagcct tcatgcggga atcctggcgg aatataaaga atatcgccag
    84181 gctccagctc ttcatcgatg atggcttcga acggatcgac ctgtaacagg tccgggtgcg
    84241 ggcagtgctg tttcatttgc agcttttcgc ccactcgcca gcgacgacga ccggtaccct
    84301 gaatgataaa cacgtcgtac tgatcgagat gcgggccgac gccgccgccc ggtacagaaa
    84361 aagagatcat cagatcatca atacgccagt ccggtagttc acggaacggt cgcattagcg
    84421 cggcggtcgg ctcatgccag tggttcactg cttgcactaa caatgaccag ttagtttcac
    84481 cgagatgatc gtagctttcg aatggaccgt ggctgacctg ccatttgcca tcctgatgac
    84541 tgaccagtcg actgtcgact tcgctttcca tcgccagacc cgccaactcg tctggagaga
    84601 ttgggtcaat aaaattatta aagccgcgtt ttaacaccac cgggcgtttc tgccagtgac
    84661 gttcaagaaa atcgggccag ttaagagtga gttggtattc catgttaagc atccacaggc
    84721 tggtatctgc aaccgattat aacggatgct taacgtaatg cgtgaagtat ggacatattt
    84781 attcatcttt cggcgcagaa tgctggcgac caaaaatcac ttccatccgc gcaccgccca
    84841 gcatgctctc tccggcgacg attttaccct catattgctc ggtgatttcg cgggctaccg
    84901 ccagccccac accttgccca gggcgtaaag tatcaacccg ttgaccacgg tcgaaaatga
    84961 cctctcgctt gcttaatgga ataccggggc catcatcctc gaccacaata tagagatgct
    85021 cgtcggtttg ccttgcagaa atttcgacaa actcgaggca atatttacag gcgttatcca
    85081 gcacgttgcc catcacttcg acaaaatcat tctgctcacc aacaaagctg atttctggcg
    85141 agatatcgag agagatattg acccctttgc gttgatacac tttgttcagc gccgaggtga
    85201 gattgtccaa cagtggcgcg accggatgca gctcgcggct aagcaatgtc ccgccgcgca
    85261 tactggcacg atgcaggtag tagccaattt gctgtgaaat gcggctgatt tgctccagca
    85321 ttaccggctc agcatcactg acgctcatct tctcactacg cagagaacgc agcgtacttt
    85381 gcagcaccgc cagtggcgtt ttcagactat gggtcaggtc ggtgagcgtt gtacgatatt
    85441 tgtcgtaacg ttcgcgttca ctttttaaca gtcggttcag gtttcgtacc agactggtca
    85501 gttctcgcgt tgtggctgga ttgagcaatt cgcggttatg ttcttccagt tcgcggactt
    85561 cttttgccag ggcttcgatg gggcgtaaac tccaccaggc ggcgacccac agcagcggga
    85621 tcactaacag cagattggct gagagcacat agataaacca gctccagacc atataggaac
    85681 tttttagctc caccggaatg gtatccacca ccacaatggt taactttggc atccgcgatg
    85741 ttgccgggta gacgtttacc gccaccgagt gggtcatctc cgcgtcgtca tcatcttccc
    85801 gcacttcctg caactgttgc tgtatcgaat gatctccact cagcaagagg ctggtatcgt
    85861 taacatccgc ttcaatttca tgaaaaccat tcgatttcag ccagtcaggc tggatcatct
    85921 tcatcagcca gggcacgtca cgttgcgccc ataaaagctg cccgttctca tcataaatta
    85981 gtgtcatcgt ggggctttgc ttgtcgatat tttcgggtaa ctcgacatgc aacttattgt
    86041 tttcccactt cgcaagggta tagaacagat tgctctcgcc acgtaacagc cgaaacgtgg
    86101 ttttatcgaa actgacgcta taaccgatca gcgcaaccat tccgtaggca agcgaaagca
    86161 ccaatactac tgctgccgtt gctaacagaa aacgtacccg cagcgagagc gggaaaaaaa
    86221 gatgcagtaa ttttttcatc agcgcaattc gaacagatag ccctggccgc gaacggtggt
    86281 aatcacttct tgcggatatt gtgcctgtat ttttttgcgc agacgtccca tcagtacatc
    86341 tatggtatga ctttcccgca gctccgcatc cgggtagagt tgtagcatta acgaatcttt
    86401 gctgaccact ttgccattat tgcgtatcaa cgtttccata atggtgtatt cgaacgcggt
    86461 cagtttgatc acttcgtcat taatagataa ttcacggcgg gagagatcaa cctgaaacgg
    86521 tggtagcgaa atgacctgtg aagccagacc gctattacgc cgcattaatg cctgcattcg
    86581 cgccatcacc tcttcactat gaaacggttt agtcacgtaa tcatcagcac cggcacttaa
    86641 tacttcgact ttgtcctgcc agctctcacg ggcggttaat accagaatcg gcagtgaaac
    86701 atcattgcta cgccagcggc gaatcagtga cagaccatcc tcgtcaggca atccgagatc
    86761 gacaatcgca atatccggta aatgttcatt gagataataa tcggcttctt tggcatcttc
    86821 tgcgtcatcg acctgatgac cagcatcctg aatctgaact ttaagatggt gacgtaacaa
    86881 cgcattgtct tcaacaacca gtacgcgcat ttttatttct ccctgtctta attattaaaa
    86941 tagtgtaacg cgattatgtg gttatggggg taaacattaa ataaaccagc gggaagggga
    87001 ggtaaagtga aaaaacaaaa agtggataat cttaataagc aggccggaca gcatcgccat
    87061 ccggcactga tttgaggctt atttcagctc atcaaccatg gtgatggcgc gaccaatata
    87121 gtttgccggc gtcatcgctt tcaggcgggc tttctcttct tctggcaacg ccagaccgtc
    87181 gataaactgc ttcatgcctt cggcgtcaac gcgcttaccg cgagtcagct ctttcagctt
    87241 ctcgtacggt ttttcgatgc catagcgacg cataactgtc tggattggct cagccagcac
    87301 ttcccagttg tgatccagct cttccagcag atggtcacgg ttcacttcca gtttgcttac
    87361 gcctttcagg gtggattgat acgcaatcag cgcataaccg atacccacgc cgaggttacg
    87421 cagcacggtg gagtcggtca ggtcacgctg ccagcgggaa actggcagtt tgcttgccag
    87481 gtgctgcaat accgcgttgg aaaggcccag gttcccttcg gagttttcga agtcgatcgg
    87541 gttaacttta tgcggcatgg tggaagaacc aatctcacca gcaatggttt tctgtttaaa
    87601 gtggttaagg gcgatataac cccagacgtc acggtcaaag tcgatcagaa tggtgttgaa
    87661 gcgcgcaacg caatcaaaca gttcggcaat gtagtcgtgc ggttcgatct gggtggtgta
    87721 cgggttccac tgaataccca gcgaggtgac gaactcttcg ctgaactgat gccagtcaac
    87781 ttccgggtaa gcggcgatgt gggcgttgta gttaccgaca gcgccgttga ttttgccgag
    87841 gatctccacc tgattaagct ggcggtactg gcgctccata cggtaggcga cgtttgccat
    87901 ctctttaccg atggttgacg gcgtggctgg ctgaccgtgg gtacgggaca gcagcgggat
    87961 atcgcgatac tgaacggcga gatctttaat gccatcaatc agttgacgcc agtacggcag
    88021 gatcacttca tcacgcgcgg ttttcagcat taatgcgtgg gagaggttat tgatatcttc
    88081 cgaagtacag gcaaagtgga tgaattcaga aaccgcgtgc agttccggga tctccgccac
    88141 tttttctttc aggaaatact caaccgcttt aacgtcgtgg ttagtggtac gctcgatggt
    88201 tttgatgcgt gctgcatctt cttcgctgaa actggcgaca attgcatcaa ggtaaccgat
    88261 tgcgtcggca gcaaaagcag gaacttcctt gatcgctgcg tgcgcggcca gtttttgcag
    88321 ccaacgtact tcaacttgta cacggaattt cagcaaaccg tattcgctga aaatcccgcg
    88381 cagcgcgctg actttatcgc cgtagcgtcc atcgacaggg gaaacggcgg tcagtgagga
    88441 taattccata gatcacaact ccggggttaa atgagcaaga atttgttttg cctgagtggt
    88501 caggcgatta cgagaaaaca tcagttgcag acgaccgccg ccgacctggt gccagagcac
    88561 ggcggcgcga atgcctgcca gcagggttgc gcgaactttc gcctgcacct gtgggctttg
    88621 cagtacagca ggggaaccag tgacctgaat gcgcgggcca agcgggctga taacatcaac
    88681 atagatggca gccatcgcgc tcatcagcgt ttcggactgt aaatcgaagt gttcgagctg
    88741 gcgttgcagg ccgttgatcc ggttgcccag agtgtcgagc gtacctttcg ctgaggagag
    88801 tttgcgctca agcaccatca gactgagtgt gtagcgggtt aattcggcgt ttaagccctg
    88861 gcgactgctg gcattgagta cgccgagcaa ggtttccagc ccgacgcgca ggttggcttc
    88921 gctaccgcca aaaaccgcca gcgttgagct ggggttcata tcgataatac tgttgagtga
    88981 gacgtgtagc gcatcgccat cacaatgccc ctggtgagcg agttgttgca ccaggcgtgc
    89041 cgactgacaa ataccggcaa gggcgagggt gatgtcatag taattctttg ccacgttcac
    89101 tgcttccttg ttttaagtaa agataataat cagaccggca gcggcagacg ctgctcaata
    89161 ataccgccac cgaggcacac ttcaccgtta tagaaaacgg cagactggcc cggcgtcacg
    89221 gcggcaaccg gctcatcgaa aatcacttca atgcgatcag cgtccagcgc cttgacggtg
    89281 cacgggatgt cggtctggcg atagcgggtt tttactgtgc aacgcatagt accggtgaac
    89341 ggctcgcgat cgacccagtg cagctgctgg gcaatcagac cgacagacat cagacgcggg
    89401 tgttcatggc cttgcgcaac aatcagaatg ttgttttcga cgtctttatc gaccacatac
    89461 cacggttctt cggtgccgtc tttggtgcca ccgataccca gacctttacg ctgaccgagg
    89521 gtgtgataca tcagcccctg gtgctcacca atttcatcgc catcgacggt aatgattttg
    89581 cccggttgcg ccgggagata acggcccagg aactcgcgga atttacgctc gccgataaag
    89641 cagataccgg tagaatcttt tttcttcgcg gtgaccagac caagatcttc agcaatctta
    89701 cgcacctgcg gtttttccag ttcgccgacc gggaacaggc tttgcgcaat ttgctcatgg
    89761 ctgagcgtat aaaggaagta gctctggtct ttattgctgt ccaggccacg cagcaggcgg
    89821 ctcttgccat cgacatctgc ccgacgtacg taatgaccgg tagcgatata atcggcacct
    89881 aaatcttcgg cggcaaattc gaggaaagct ttaaatttga tctctttgtt gcacagaata
    89941 tccggatttg gcgtgcgacc ggctttatat tcggcaagga acagttcgaa gacgttgtcc
    90001 cagtactcgg cagcaaagtt aacggtgtgc agttcaatgc cgagcttgtc gcagacagcc
    90061 tgggcatcag ccagatccgc tgccgctgtg caatattcct caccgtcgtc ttcttcccag
    90121 ttcttcataa acaggccttc gacctgatat ccctgttgtt gcaacagcca ggcagaaacg
    90181 gaggaatcga caccgccgga catgccgacg attacttttt ttgcggtttc agacattgga
    90241 tcactcacga cattgaactt caaggcggcg tattctatca cgcagccttt tagatgacac
    90301 cctttgtaaa aggccagtta aaatcgccaa tcatctccag cggatagcgt tgcccgcttt
    90361 gataacaacg aatactttcc gccaccagcg gcgaacgaag atttgacgcc tgtaaaattt
    90421 cttcggcgct gacccaacgg cagcagtcga tatcgctgtc atgaggctgc gtcgggcata
    90481 tttgctcaag ctcaatggca aagaggaaac gcaaaaacgg cgttttatca ggcgcaatcc
    90541 actgatgcat gcggataaaa tgttgcggtt gcgcgctgat gccggtttct tcccacagct
    90601 cacgggcggc ggcttccact aaggtttcat ctgcttccag atgcccggcg ggctggttcc
    90661 ataacgcttt accattaatc gtctcttcaa cgactaaaaa tttgccttct gcgtgcacca
    90721 cgcaggcaac ggtaacgtgc ggtttaaaca ttttgcatcc ttaatctgtc gcgtctcgcc
    90781 attcgccatt ggcaagatta tccaaagagt aattccccat cgcatagcga atcagtcgca
    90841 gcgtggggaa accaacatgg gcggtcatgc ggcgcacctg gcgattacgt ccttcatata
    90901 aggtgatctt cagccagctg gtgggaatac ttttgcgctc acgaattggt ggattccgcg
    90961 gccataacca cgcgggttct tcaaccagct ccgcgcctgc gggcagggta gggccatcat
    91021 ttaaggttac accattgcgc aaggcttcaa gggcgtcttg tgtgggaata ccttctacct
    91081 gtacataata gattttcccg gtacgtttac ccggctgggt taaacgcgcc tgcaaagcgc
    91141 cgttattggt cagcaccagt aacccttcgc tgtcgcggtc gagacgacct gccgcataca
    91201 ccccctgaac cgggatgaat tcttttaatg ttttgcgtcc ggcttcatcg gtgaactgcg
    91261 gaagaacatc gtagggttta ttgaacagga tcacacgcgt gggctggttg tcaggtttac
    91321 gcctggtaga acgttgcgag ctgaatcgct taacctggtg atttctaaaa gaagtttttt
    91381 gcatggtatt ttcagagatt atgaattgcc gcattatagc ctaataacgc gcatctttca
    91441 tgacggcaaa caatagggta gtattgacaa gccaattaca aatcattaac aaaaaattgc
    91501 tctaaagcat ccgtatcgca ggacgcaaac gcatatgcaa cgtggtggta gacgagcaaa
    91561 ccagtagcgc tcgaaggaga ggtgaatgga aagtaaagta gttgttccgg cacaaggcaa
    91621 gaagatcacc ctgcaaaacg gcaaactcaa cgttcctgaa aatccgatta tcccttacat
    91681 tgaaggtgat ggaatcggtg tagatgtaac cccagccatg ctgaaagtgg tcgacgctgc
    91741 agtcgagaaa gcctataaag gcgagcgtaa aatctcctgg atggaaattt acaccggtga
    91801 aaaatccaca caggtttatg gtcaggacgt ctggttgcct gctgaaaccc ttgatctgat
    91861 tcgtgaatat cgcgttgcca ttaaaggccc gctgaccact ccggttggtg gcggtattcg
    91921 ctctctgaac gttgccctgc gccaggaact ggatctctac atctgcctgc gtccggtacg
    91981 ttactatcag ggcactccaa gcccggttaa acaccctgaa ctgaccgata tggttatctt
    92041 ccgtgaaaac tcggaagaca tttatgcggg tatcgaatgg aaagctgact ctgccgacgc
    92101 cgagaaagtg attaaattcc tgcgtgaaga gatgggcgtg aagaaaattc gcttcccgga
    92161 acattgcggc atcggtatta agccgtgttc tgaagaaggc accaaacgtc tggttcgtgc
    92221 agcgatcgaa tacgcaattg ctaacgatcg tgactctgtg accctggtgc acaaaggcaa
    92281 catcatgaag ttcaccgaag gcgcgtttaa agactggggc taccagctgg cgcgtgaaga
    92341 atttggcggt gaactgatcg acggcggccc gtggctgaaa gttaaaaacc cgaataccgg
    92401 caaagagatc gtcattaaag acgtgattgc tgatgcattc ctgcaacaga tcctgctgcg
    92461 tccggctgaa tatgatgtta tcgcctgtat gaacctgaac ggtgactaca tttctgacgc
    92521 cctggcagcg caggttggtg gtatcggtat cgcccctggc gcaaacatcg gtgacgaatg
    92581 cgccctgttt gaagccaccc acggtactgc gccgaaatat gccggacagg acaaagtaaa
    92641 cccaggctct attattctct ccgctgagat gatgctgcgc catatgggct ggactgaagc
    92701 cgcagacctg attgttaaag gtatggaagg cgcaatcaac gccaagaccg taacctatga
    92761 cttcgaacgt ctgatggaag gcgctaaact gctgaaatgc agcgagtttg gtgacgcgat
    92821 catcaaaaat atgtaattac cacatgtgtt aaatattata acgggcgtat aacacgcccg
    92881 ttgttttatg atgatgtaaa atcttcccca aaactttccc caaaaccctt ccccaaaact
    92941 ggctattttc tatgctgttt tgatatctac gataatccag tctttaccac gatcatcatt
    93001 gtatcggtcg gtcatttttc tggatttatg gcctaacaac ttttgcgtat ccagaccctg
    93061 ttcccgatat aaccgttctg acagagatcg ctgctcatga aatgtgggcg cagttccttg
    93121 ctcccatttt atgccacatt tttccctggc ctttttaaaa gccgttgtca gagtattcgc
    93181 agaaacctgg tctcctctgt ttgcttgaga ggttgtgtga cggtaatgga ccagatattt
    93241 actaacaaca gcatccctgc actgagatat aacttcacga agggtaatat tcagagcatc
    93301 gcatttcagg ttaagcggaa tagcaagttt tgaaccggtt ttttcctgag taatgtgcaa
    93361 catgtcgtcc tagatatcag agaatttcaa attgcagata tcgcctaaac gttgtccagt
    93421 gacaagagca agtagcatcc cgcattttaa atagggctgc cgtctgctta cgctgtcaaa
    93481 tattgcctgc cattcgggca gtgataatct ttggcggttt actcgatttc gcggttgttt
    93541 tgttgcctgc gctgggttaa atcctggcgg aacatgtcct gcgtgttgtg cttctttgaa
    93601 gacgtcgatt aacaccattc tcacgacttg cgccatcctg ttatgacctt cagcctttac
    93661 agcatcaatt atttcggcaa tatcaagtgc ggtaatatcc ttgaggtgtt gcattccaca
    93721 atgctcacgg aaaagacgaa tgggtttgcc tttttgccga taggagttgg gtcttagttc
    93781 attatgttgc agcctgtcct cctggataga aatatattta tcaagccatt ctgtcaccgt
    93841 aatgtctgag cgcctgcctt tcattctttc cagacgctca ttgacgctta atatttgtcg
    93901 ggtacgttgt tcagcaataa tggtatttgc ttcagtagca acttgttttg cttcattctc
    93961 atcagttcct aagctatgaa aacgaccgga tagtggatgt ttgtattgcc aatatacctt
    94021 tccggttcgc ttatctaatt tgcaatataa attgggtata gagattttgt gagatcgggg
    94081 tctagcagcc atcagcgatt atccgttgga gttttgggtt tgcgtttatt gggagttgcg
    94141 gttctgcaag cgttcctaca aaacgggaat ttcggtcaat catccagtag cgaccaactt
    94201 ttatagcggg tggggccatc attttccctt gcgcgtattt tttcagaact cgctcacttg
    94261 gtgctaagtc cccaaattct tctttagccc agtcctgtaa agtgattagt cgagacattt
    94321 gtcctcctct tagctgctga gggagtttgt gaccgatata tctgacatga tattaagctc
    94381 atggcaggta catctcttga ctggtcatag agataaattt aatgctgaga aatgcagtat
    94441 tgaatttatc aatttttcta tttcctgcgt atggcacgta acttcttaat gtgttctgcc
    94501 gtttcgatct cttctgctat ccgatctgca tcagctttat tcacaggttc aaagtcatga
    94561 ttaaagcgga acatgctggc gatacatgtt ctgccttttc ggatgtagtg aactttgttg
    94621 tgggtagaac gcaggatttt gcagggagtg ccgtggtggt cgacgtacca ggtgttagga
    94681 aaaatgattc tgaacatttt tacacctcag ttggacgatg ttgaaatttg ctgctttgag
    94741 gccatcacag tccccattgt ttgttcttaa gttcgatctc ctcctggcaa cttgcacaag
    94801 tccgacaacc ctgaacggcc aggcgtcttc gttcatctat cggatcgcca cactcacaac
    94861 aatgagtggc agatatagcc tggtggttca ggcggcgcat ttttattgct gtgttgcgct
    94921 gtaattcttc aatttctgat gctgaatcaa tgatgtctgc catctttcat taatccctga
    94981 attgttggtt aatacgcttg agggtgaatg cgaataataa aaaaggagcc tgtagctcca
    95041 tgatgatttt gtttttcatg ttcaccgttc cttaaagacg ccgtttaaca tgccgatcgc
    95101 caggcttaaa tgagtcggtg tgaatcccat cagcgttacc gtttcgcggt gcttcttcag
    95161 tacgctacgg caaatgtcat cgacgttttt atccggaaac tgctgtctgg cttttttgat
    95221 ttcataatta gcctgacggg caatactgcg aagggcgttt tcttgctgag gtgtcattga
    95281 acaagtccca tgtcggcaag cataagcaca cagaatatga agcccgctgc cagaaaaatg
    95341 cattcagtgg ttgtcatacc tggtctctct catctgcttc tgctttcgcc accatcattt
    95401 ccagcttttg tgaaagggat gtggttaacg tatgaaattc ttcgtctgtt tctactggta
    95461 ttggcacaaa cctgactcca atttgagcga ggctatgtgc catctcgata ctcgttctta
    95521 actcaacggg agatgctttg tgcatacagc ccctcgttta ttatttatct cctcagccag
    95581 ccgctgggct ttcagtggat tttggataac agaaaggccg ggaaataccc agcctcgctt
    95641 tgtaacggag tagacgaaag tgatcgcgcc tacccggata ttatcgtgag gatgcgtcat
    95701 cgccattgct ccccaaatac aaaaccaatt tcagccagtg cctcgtccat tttttcgatg
    95761 aactccggca ccatctcgtc aaaactcgcc atgtactttt catcccgctc aaccacgaca
    95821 taatgcagtc cttcacgctt catacgcggg tcatagttgg caaagtacca ggcatctttt
    95881 cgcgtcaccc acatgctgta ctgcacctgg gccatgtaag ccgactttat ggcctcgaaa
    95941 ccaccgagcc ggaacttcat gaaatcccgg gaggtaaacg ggcatttcag ctcaaggcca
    96001 ttgccgtcac tgcataaacc atcgggagag caggcggtgc gcatactttc gtcgcgatag
    96061 atgatcgggg attcagtaa
//
LOCUS       DAZFFE010000005        90473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFFE010000005 DAZFFE010000000
VERSION     DAZFFE010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 90473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.005"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1780)
                     /locus_tag="ACHJ69_000436"
     CDS             complement(<1..1780)
                     /locus_tag="ACHJ69_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFO5013505.1"
                     /translation="MSQLDEAILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWKTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFA
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPMLTSATVIKLLHQRDENGEDRVTGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAGGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQHGRQVFMDFNRNPEPVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQQMNPLSISLYKMHGYDLITQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLAAPA
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSA"
     gene            complement(1777..3318)
                     /locus_tag="ACHJ69_000437"
     CDS             complement(1777..3318)
                     /locus_tag="ACHJ69_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO5013506.1"
                     /translation="MRKLSTAETLAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDSKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPSGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGTIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFSIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDRYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVALVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPFMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            complement(3328..4104)
                     /locus_tag="ACHJ69_000438"
     CDS             complement(3328..4104)
                     /locus_tag="ACHJ69_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HFO5013507.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NNTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNEVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPHAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYSHQLLAGLMSATQDCQQATHAFAEKAGVSFHNQ"
     gene            complement(4114..4956)
                     /locus_tag="ACHJ69_000439"
     CDS             complement(4114..4956)
                     /locus_tag="ACHJ69_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO5013508.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQSEDLQQIARQLAASNMNVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPMGTKDLYEAREQVKQGIRQLLPHSKEVGVPIALEPLHPMTAANRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            complement(4966..5757)
                     /locus_tag="ACHJ69_000440"
     CDS             complement(4966..5757)
                     /locus_tag="ACHJ69_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFO5013509.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHATQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            5970..6617
                     /locus_tag="ACHJ69_000441"
     CDS             5970..6617
                     /locus_tag="ACHJ69_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO5013510.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYISNP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKNNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTARE"
     gene            complement(6601..7239)
                     /gene="ytfB"
                     /locus_tag="ACHJ69_000442"
     CDS             complement(6601..7239)
                     /gene="ytfB"
                     /locus_tag="ACHJ69_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO5013511.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTHTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GADKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            7457..8077
                     /gene="fklB"
                     /locus_tag="ACHJ69_000443"
     CDS             7457..8077
                     /gene="fklB"
                     /locus_tag="ACHJ69_000443"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO5013512.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQTMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            8386..9798
                     /gene="cycA"
                     /locus_tag="ACHJ69_000444"
                     /gene_synonym="dagA"
     CDS             8386..9798
                     /gene="cycA"
                     /locus_tag="ACHJ69_000444"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO5013513.1"
                     /translation="MVDQVKVVADDQSPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTINLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPGLSDWVASLSVIILLLVLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFSLIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVLVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(9843..10505)
                     /gene="ytfE"
                     /locus_tag="ACHJ69_000445"
     CDS             complement(9843..10505)
                     /gene="ytfE"
                     /locus_tag="ACHJ69_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO5013514.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVDVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMEHISLENNVLFPRALA
                     GE"
     gene            complement(10613..11578)
                     /gene="ytfF"
                     /locus_tag="ACHJ69_000446"
     CDS             complement(10613..11578)
                     /gene="ytfF"
                     /locus_tag="ACHJ69_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO5013515.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNMQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(11686..12546)
                     /gene="qorB"
                     /locus_tag="ACHJ69_000447"
     CDS             complement(11686..12546)
                     /gene="qorB"
                     /locus_tag="ACHJ69_000447"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO5013516.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAADVKFIAYT
                     SLLHADSSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAVAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKPIT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            12635..13015
                     /gene="ytfH"
                     /locus_tag="ACHJ69_000448"
     CDS             12635..13015
                     /gene="ytfH"
                     /locus_tag="ACHJ69_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO5013517.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(13144..15087)
                     /gene="cpdB"
                     /locus_tag="ACHJ69_000449"
     CDS             complement(13144..15087)
                     /gene="cpdB"
                     /locus_tag="ACHJ69_000449"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO5013518.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYTQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            15277..16017
                     /gene="cysQ"
                     /locus_tag="ACHJ69_000450"
     CDS             15277..16017
                     /gene="cysQ"
                     /locus_tag="ACHJ69_000450"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO5013519.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQCYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(16007..16564)
                     /gene="ytfJ"
                     /locus_tag="ACHJ69_000451"
     CDS             complement(16007..16564)
                     /gene="ytfJ"
                     /locus_tag="ACHJ69_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO5013520.1"
                     /translation="MTLSKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            16889..17095
                     /gene="ytfK"
                     /locus_tag="ACHJ69_000452"
     CDS             16889..17095
                     /gene="ytfK"
                     /locus_tag="ACHJ69_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO5013521.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(17173..18516)
                     /gene="paeA"
                     /locus_tag="ACHJ69_000453"
     CDS             complement(17173..18516)
                     /gene="paeA"
                     /locus_tag="ACHJ69_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO5013522.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(18839..19477)
                     /gene="msrA"
                     /locus_tag="ACHJ69_000454"
     CDS             complement(18839..19477)
                     /gene="msrA"
                     /locus_tag="ACHJ69_000454"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO5013523.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            19683..21416
                     /gene="tamA"
                     /locus_tag="ACHJ69_000455"
     CDS             19683..21416
                     /gene="tamA"
                     /locus_tag="ACHJ69_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO5013524.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGELEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSFDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            21413..25192
                     /gene="tamB"
                     /locus_tag="ACHJ69_000456"
     CDS             21413..25192
                     /gene="tamB"
                     /locus_tag="ACHJ69_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO5013525.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNVKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEAAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVSTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYAISMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     TVHVGDNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTEGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            25195..25536
                     /gene="ytfP"
                     /locus_tag="ACHJ69_000457"
     CDS             25195..25536
                     /gene="ytfP"
                     /locus_tag="ACHJ69_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO5013526.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            25686..27158
                     /locus_tag="ACHJ69_000458"
     CDS             25686..27158
                     /locus_tag="ACHJ69_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFO5013527.1"
                     /translation="MKKSLLAVMLTGLFALVSLPALGNVNLEQLKQKAESGEAKAQLE
                     LGYRYFQGNETTKDLTLAMDWFRRAAEQGYTPAEYVLGLRYMNGEGVPQDYAQAVIWY
                     KKAALKGLPQAQQNLGVMYHEGNGVKVDKAESVKWFRLAAEQGRDSGQQSMGDAYFEG
                     DGVTRDYVMAREWYSKAAEQGNVWSCNQLGYMYSRGLGVERNDAISAQWYRKSATSGD
                     ELGQLHLADMYYFGIGVTQDYTQSRVLFSQSAEQGNSIAQFRLGYILEQGLAGAKEPL
                     KALEWYRKSAEQGNSDGQYYLAHLYDKGAEGVAKNREQAISWYTKSAEQGDATAQANL
                     GAIYFRLGSEEEHKKAVEWFRKAAAKGEKAAQFNLGNALLQGKGVKKDEQQAAIWMRK
                     AAEQGLSAAQVQLGEIYYYGLGVERDYVQAWAWFDTASTNDMNLFGTENRNITEKKLT
                     AKQLQQAELLSQQYIEKYAPEAWARMQKLKAQSAVKTGNK"
     gene            complement(27572..28102)
                     /gene="ppa"
                     /locus_tag="ACHJ69_000459"
     CDS             complement(27572..28102)
                     /gene="ppa"
                     /locus_tag="ACHJ69_000459"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO5013528.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            28412..29368
                     /gene="ytfQ"
                     /locus_tag="ACHJ69_000460"
     CDS             28412..29368
                     /gene="ytfQ"
                     /locus_tag="ACHJ69_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO5013529.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            29508..31010
                     /gene="ytfR"
                     /locus_tag="ACHJ69_000461"
     CDS             29508..31010
                     /gene="ytfR"
                     /locus_tag="ACHJ69_000461"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO5013530.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALAGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEIEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKLQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHTEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            31024..32046
                     /gene="ytfT"
                     /locus_tag="ACHJ69_000462"
     CDS             31024..32046
                     /gene="ytfT"
                     /locus_tag="ACHJ69_000462"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO5013531.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RNRDKT"
     gene            32033..33028
                     /gene="yjfF"
                     /locus_tag="ACHJ69_000463"
     CDS             32033..33028
                     /gene="yjfF"
                     /locus_tag="ACHJ69_000463"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO5013532.1"
                     /translation="MIKRNLPLMITIGVFVLGYIYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSTMGLLMLAVVVIGILLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(33061..34059)
                     /gene="fbp"
                     /locus_tag="ACHJ69_000464"
     CDS             complement(33061..34059)
                     /gene="fbp"
                     /locus_tag="ACHJ69_000464"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO5013533.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            34235..35608
                     /gene="mpl"
                     /locus_tag="ACHJ69_000465"
     CDS             34235..35608
                     /gene="mpl"
                     /locus_tag="ACHJ69_000465"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO5013534.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(35766..36317)
                     /gene="yjgA"
                     /locus_tag="ACHJ69_000466"
     CDS             complement(35766..36317)
                     /gene="yjgA"
                     /locus_tag="ACHJ69_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO5013535.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            36411..37763
                     /gene="pmbA"
                     /locus_tag="ACHJ69_000467"
     CDS             36411..37763
                     /gene="pmbA"
                     /locus_tag="ACHJ69_000467"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO5013536.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPYAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGAVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIVKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            37946..38332
                     /gene="cybC"
                     /locus_tag="ACHJ69_000468"
     CDS             37946..38332
                     /gene="cybC"
                     /locus_tag="ACHJ69_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO5013537.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AVQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(38377..38841)
                     /gene="nrdG"
                     /locus_tag="ACHJ69_000469"
     CDS             complement(38377..38841)
                     /gene="nrdG"
                     /locus_tag="ACHJ69_000469"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO5013538.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(38999..41137)
                     /gene="nrdD"
                     /locus_tag="ACHJ69_000470"
     CDS             complement(38999..41137)
                     /gene="nrdD"
                     /locus_tag="ACHJ69_000470"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO5013539.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNSSLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEICKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDTSRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(41531..43186)
                     /gene="treC"
                     /locus_tag="ACHJ69_000471"
     CDS             complement(41531..43186)
                     /gene="treC"
                     /locus_tag="ACHJ69_000471"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO5013540.1"
                     /translation="MTHLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRYPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTSYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWTNGDNAGFTAGEPWIGLGDNYQEINVEAALA
                     DESSVFYTYQKLIALRKQEAVLTWGDYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     TQPWQPGKMLGNWQLVMHNYEEASPQPCTMTLRPFEAVWWLQK"
     gene            complement(43236..44657)
                     /gene="treB"
                     /locus_tag="ACHJ69_000472"
     CDS             complement(43236..44657)
                     /gene="treB"
                     /locus_tag="ACHJ69_000472"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO5013541.1"
                     /translation="MMSKINQTDIDRLIEMVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GIFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(44776..45723)
                     /gene="treR"
                     /locus_tag="ACHJ69_000473"
     CDS             complement(44776..45723)
                     /gene="treR"
                     /locus_tag="ACHJ69_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFO5013542.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            45908..45961
                     /gene="mgtL"
                     /locus_tag="ACHJ69_000474"
     CDS             45908..45961
                     /gene="mgtL"
                     /locus_tag="ACHJ69_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO5013543.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            46102..48798
                     /gene="mgtA"
                     /locus_tag="ACHJ69_000475"
     CDS             46102..48798
                     /gene="mgtA"
                     /locus_tag="ACHJ69_000475"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO5013544.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNAAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNLAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(49004..49390)
                     /gene="ridA"
                     /locus_tag="ACHJ69_000476"
     CDS             complement(49004..49390)
                     /gene="ridA"
                     /locus_tag="ACHJ69_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO5013545.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(49463..49924)
                     /gene="pyrI"
                     /locus_tag="ACHJ69_000477"
     CDS             complement(49463..49924)
                     /gene="pyrI"
                     /locus_tag="ACHJ69_000477"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO5013546.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(49937..50872)
                     /gene="pyrB"
                     /locus_tag="ACHJ69_000478"
     CDS             complement(49937..50872)
                     /gene="pyrB"
                     /locus_tag="ACHJ69_000478"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO5013547.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(50876..51010)
                     /gene="pyrL"
                     /locus_tag="ACHJ69_000479"
     CDS             complement(50876..51010)
                     /gene="pyrL"
                     /locus_tag="ACHJ69_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO5013548.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(51100..51588)
                     /locus_tag="ACHJ69_000480"
     CDS             complement(51100..51588)
                     /locus_tag="ACHJ69_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFO5013549.1"
                     /translation="MKEYDDYSAKEKKQLAVCQRLITEKSYLSQEEIRRDLQNHGFDS
                     ISQSTVSRLLKLLGVIKIRNTKGQKIYSVNPQLLPTPDAGRSVAEMVLSVEHNGEFIL
                     IHTVAGYGRAVARILDFHALPEILGVIAGSNIVWVAPRVVKRTALVHKQINYLLKLNI
                     YS"
     gene            complement(51704..53107)
                     /locus_tag="ACHJ69_000481"
     CDS             complement(51704..53107)
                     /locus_tag="ACHJ69_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFO5013550.1"
                     /translation="MGKFKFPTAYTILFVLIALVAAMTWIVPAGKYQMVTNAALGKEV
                     PVAGTYAPVEAQPQGITAVLLAPIDGLYNHQTYTAGAIDVALFVLIIGGFLGVVNKTG
                     AIDAGIERVTTRLNGREEWMIPILMALFAAGGTIYGMAEESLPFYTLLVPVMMAARFD
                     PLVAAATVLLGSGIGTLGSTINPFATVIAANAAGIPFTQGILLRVILLLVGYVICVYW
                     VMRYARKVRNSPESSIVADKMAENQAHFLGNRSETMLEFTPTRKAILILFAASFAFMI
                     YGVAVLGWWMAEISAVFLAAAVIVGVIARMGEETFTSTFIDGARDLLGVALIIGIARG
                     IVVVMDNGMITHTILHSAENLVSGLSTTVFINVTYWLEVLLSFLVPSSSGLAVLTMPI
                     LAPLADFAHVQRDLVVTAYQSASGVVNLITPTSAVVMGGLAIARVPWVRYLRWVAPLL
                     LILTMLNMIVLSIAAMI"
     gene            complement(53164..54168)
                     /gene="argF"
                     /locus_tag="ACHJ69_000482"
     CDS             complement(53164..54168)
                     /gene="argF"
                     /locus_tag="ACHJ69_000482"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO5013551.1"
                     /translation="MATSLKNRNFLKLLDYTPAEIQYLIDLAINLKAAKKSGNEKQTL
                     VGKNIALIFEKSSTRTRCAFEVAAFDQGAQVTYIGPSGSQIGHKESMKDTARVLGRMY
                     DGIEYRGYGQNIVEELGEFAGVPVWNGLTNEFHPTQILADLMTMLEHAPGKTLPELSF
                     AYLGDARNNMGNSLMVGAAKMGMDIRLVAPKSFWPDEALVTHCREIASVTGARITLTE
                     DVEEGVYDVDFLYTDVWVSMGEPKEAWAERVSLMAPYQINQQVITATRNPEVKFMHCL
                     PAFHNEHTTVGREIEMAYGLKGLEVTEEVFESAHSIVFDEAENRMHTIKAVMVATLGD
                     "
     gene            complement(54192..55124)
                     /gene="arcC"
                     /locus_tag="ACHJ69_000483"
     CDS             complement(54192..55124)
                     /gene="arcC"
                     /locus_tag="ACHJ69_000483"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO5013552.1"
                     /translation="MENKPTLVIALGGNALLKRGEPLEAEIQRKNIDLAAKTIAQLTQ
                     HWRVVLVHGNGPQVGLLALQNSAYAHVAPYPLDILGAESQGMIGYMLQQALKNQLPQR
                     EISVLLTQVEVDANDPAFSNPTKYIGPIYDHAQTQVLQAEKDWVFKADGHSFRRVVPS
                     PQPKRIVERDAIQTLIAHDHLVICNGGGGVPVVEKADGYHGIEAVIDKDLSAALLASQ
                     IHADALLILTDADAVYLDWGKPTQRPLAQVTPELLNEMQFDAGSMGPKVTACAKFVSQ
                     CRGIAGIGSLADGPEILAGDKGTLIRLDTPHNHA"
     gene            complement(55135..56355)
                     /gene="arcA"
                     /locus_tag="ACHJ69_000484"
     CDS             complement(55135..56355)
                     /gene="arcA"
                     /locus_tag="ACHJ69_000484"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFO5013553.1"
                     /translation="MEKHYVGSEIGQLRSVMLHRPNLSLKRLTPSNCQELLFDDVLSV
                     ERAGEEHDIFANTLRQQGIEVLLLTDLLTQTLDIPEAKSWLLETQISDYRLGPTFATD
                     VRTWLAEMSHRDLARHLSGGLTYSEIPASIKNMVVDTHDINDFIMKPLPNHLFTRDTS
                     CWIYNGVSINPMAKPARQRETNNLRAIYRWHPQFAGGEFIKYFGDENINYDHATLEGG
                     DVLVIGRGAVLIGMSERTTPQGIEFLAQALFKHRQAERVIAVELPKHRSCMHLDTVMT
                     HIDIDTFSVYPEVVRPDVNCWTLTPDGHGGLKRTQESTLLHAIEKALGIDQVRLITTG
                     GDAFEAEREQWNDANNVLTLRPGVVVGYERNIWTNEKYDKAGITVLPIPGDELGRGRG
                     GARCMSCPLHRDGI"
     gene            57033..57485
                     /locus_tag="ACHJ69_000485"
     CDS             57033..57485
                     /locus_tag="ACHJ69_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO5013554.1"
                     /translation="MIVGNIHHLQSWLPEELREAIEYIKSHVSDETAKGKHAIDGDRL
                     FYLISEDTTEPGELHRAEYHARYLDIQIVLKGQEGMTFSTQPAGVPETDWLADKDIAF
                     IGQGIDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKLLKS"
     gene            complement(57530..58534)
                     /gene="argF"
                     /locus_tag="ACHJ69_000486"
     CDS             complement(57530..58534)
                     /gene="argF"
                     /locus_tag="ACHJ69_000486"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO5013555.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEARLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVAECSALAQKHGGKITLTED
                     IASGVKGADFIYTDVWVSMGEPKEKWAERIALLRDYQVNSKMMALTGNSQVKFLHCLP
                     AFHDEQTTLGKKMAAEFGLYGGMEVTDEVFESPASIVFDQAENRMHTIKAVMVATLAK
                     "
     gene            58696..59112
                     /gene="rraB"
                     /locus_tag="ACHJ69_000487"
     CDS             58696..59112
                     /gene="rraB"
                     /locus_tag="ACHJ69_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO5013556.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGIRH"
     gene            complement(59289..59792)
                     /gene="yjgM"
                     /locus_tag="ACHJ69_000488"
     CDS             complement(59289..59792)
                     /gene="yjgM"
                     /locus_tag="ACHJ69_000488"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5013557.1"
                     /translation="MNVVASPALRLRKLTVADNPAIAHVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLRKL"
     gene            59985..61172
                     /locus_tag="ACHJ69_000489"
     CDS             59985..61172
                     /locus_tag="ACHJ69_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HFO5013558.1"
                     /translation="MNDVNIGKDNSRHSFVFTGKGGEYFLICLVNFLLTIITLGIYGP
                     WALIKCRRYIYQHVTLKGQPFSYKGTGGAIFVSMLLIVVVYLLSISCFAGQHFALGLF
                     LFALLICGIPCMAVKSLQYQANMTSLNDIRFGFNCSMMRAWWVMLGLPVLLALVFWFA
                     LYLIAQVTTSIGGVFFNLVALSLLSAIGLGVVHGITYSKWMPLLGNNATFGIHKFSIQ
                     VNVKECIKGCMLAILTMVPFIIVIGIMIAPVFQQLMMMTMLGRSDAGSEFVLQYYPQI
                     MASYFLYFVAILVFASYLYVTLRNLFLNNLTLANGTIRFHSSVTAIGMLLRMLAVLMG
                     SSITCGLAYPWLKMWMVSWIANNTHVQGDLDSLELTNDDKPQDSGSLMWISRGIMPYV
                     PFI"
     gene            complement(61219..64074)
                     /gene="valS"
                     /locus_tag="ACHJ69_000490"
     CDS             complement(61219..64074)
                     /gene="valS"
                     /locus_tag="ACHJ69_000490"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO5013559.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIVDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(64074..64517)
                     /gene="holC"
                     /locus_tag="ACHJ69_000491"
     CDS             complement(64074..64517)
                     /gene="holC"
                     /locus_tag="ACHJ69_000491"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO5013560.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSNPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(64775..66286)
                     /gene="pepA"
                     /locus_tag="ACHJ69_000492"
     CDS             complement(64775..66286)
                     /gene="pepA"
                     /locus_tag="ACHJ69_000492"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO5013561.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            66440..66490
                     /gene="ytgA"
                     /locus_tag="ACHJ69_000493"
     CDS             66440..66490
                     /gene="ytgA"
                     /locus_tag="ACHJ69_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO5013562.1"
                     /translation="MSIIILATISTTQENK"
     gene            66553..67653
                     /gene="lptF"
                     /locus_tag="ACHJ69_000494"
     CDS             66553..67653
                     /gene="lptF"
                     /locus_tag="ACHJ69_000494"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO5013563.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            67653..68735
                     /gene="lptG"
                     /locus_tag="ACHJ69_000495"
     CDS             67653..68735
                     /gene="lptG"
                     /locus_tag="ACHJ69_000495"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO5013564.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(68896..70398)
                     /gene="yjgR"
                     /locus_tag="ACHJ69_000496"
     CDS             complement(68896..70398)
                     /gene="yjgR"
                     /locus_tag="ACHJ69_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO5013565.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGVAEEGTSSEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASIEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(70476..71474)
                     /gene="idnR"
                     /locus_tag="ACHJ69_000497"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             complement(70476..71474)
                     /gene="idnR"
                     /locus_tag="ACHJ69_000497"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFO5013566.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDTLSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            complement(71541..72860)
                     /gene="idnT"
                     /locus_tag="ACHJ69_000498"
     CDS             complement(71541..72860)
                     /gene="idnT"
                     /locus_tag="ACHJ69_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFO5013567.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            complement(72925..73689)
                     /gene="idnO"
                     /locus_tag="ACHJ69_000499"
     CDS             complement(72925..73689)
                     /gene="idnO"
                     /locus_tag="ACHJ69_000499"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFO5013568.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            complement(73713..74744)
                     /gene="idnD"
                     /locus_tag="ACHJ69_000500"
     CDS             complement(73713..74744)
                     /gene="idnD"
                     /locus_tag="ACHJ69_000500"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFO5013569.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSKLHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTEMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            74961..75524
                     /gene="idnK"
                     /locus_tag="ACHJ69_000501"
     CDS             74961..75524
                     /gene="idnK"
                     /locus_tag="ACHJ69_000501"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO5013570.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQDDEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            complement(75528..76547)
                     /gene="ahr"
                     /locus_tag="ACHJ69_000502"
     CDS             complement(75528..76547)
                     /gene="ahr"
                     /locus_tag="ACHJ69_000502"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO5013571.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLIAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLPVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            76743..76827
                     /locus_tag="ACHJ69_000503"
     tRNA            76743..76827
                     /locus_tag="ACHJ69_000503"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:76777..76779,aa:Leu,seq:caa)
     gene            77014..78279
                     /locus_tag="ACHJ69_000504"
     CDS             77014..78279
                     /locus_tag="ACHJ69_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5013572.1"
                     /translation="MALTDIKVRAAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARVRRDEARNLLANGVDPGDKKKNDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEV
                     AARLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASCNRQHRPALELKRIPELLTKIDS
                     YTGRPLTRWAIELTLLIFIRSSELRFARWSEIDFEASIWTIPPEREPIPGVKHSHRGS
                     KMRTTHLVPLSTQALAILKQIKQFYGAHDLIFIGDHDSHKPMSENTVNSALRVMGYDT
                     KVEVCGHGFRTMACSSLVESGLWSRDAVERQMSHMERNSVRAAYIHKAEHLEERRLML
                     QWWADFLDVNRERFISPFEYAKINNPLKQ"
     gene            complement(78596..79119)
                     /locus_tag="ACHJ69_000505"
                     /pseudo
     CDS             complement(78596..79119)
                     /locus_tag="ACHJ69_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            79706..79935
                     /locus_tag="ACHJ69_000506"
                     /pseudo
     CDS             79706..79935
                     /locus_tag="ACHJ69_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            80966..82183
                     /gene="nanX"
                     /locus_tag="ACHJ69_000507"
     CDS             80966..82183
                     /gene="nanX"
                     /locus_tag="ACHJ69_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5013573.1"
                     /translation="MATAWYKQVNPPQRKALFSAWLGYVFDGFDFMMIFYILHIIKAD
                     LGITDIQATLIGTVAFIARPIGGGFFGAMADKYGRKPMMMWAIIIYSVGTGLSGIATN
                     LYMLAVCRFIVGLGMSGEYACASTYAVESWPKNLQSKASAFLVSGFSVGNIIAAQIIP
                     QFAEVYGWRNSFFIGLLPVLLVLWIRKSAPESQEWIEDKYKDKSTFLSVFRKPHLSIS
                     MIVFLVCFCLFGANWPINGLLPSYLADNGVNTVVISTLMTIAGLGTLTGTIFFGFVGD
                     KIGVKKAFVVGLITSFVFLCPLFFISVKNSSLIGLCLFGLMFTNLGIAGLVPKFIYDY
                     FPTKLRGLGTGLIYNLGATGGMAAPVLATYISGYYGLGVSLFIVTVAFSALLILLVGF
                     DIPGKIYKLSVAK"
     gene            82195..83313
                     /gene="nanY"
                     /locus_tag="ACHJ69_000508"
                     /gene_synonym="yjhC"
     CDS             82195..83313
                     /gene="nanY"
                     /locus_tag="ACHJ69_000508"
                     /gene_synonym="yjhC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,7-anhydro-N-acetylneuraminate hydratase"
                     /protein_id="HFO5013574.1"
                     /translation="MINYGVVGVGYFGAELARFMNMHDNAKITCVYDPENGENIAREL
                     QCINMSSLDALVSSKLVDCVIVATPNYLHKEPVIKAAKNKKHVFCEKPIALSYEDCVD
                     MVKACKEAGVTFMAGHIMNFFNGVQYARKLIKEGVIGEILSCHTKRNGWENKQERLSW
                     KKMKEQSGGHLYHHIHELDCVQHLLGEIPETVTMIGGNLAHSGPGFGNEDDMLFMTLE
                     FPSGKLATLEWGSAFNWPEHYVIINGTKGSIKIDMQETAGSLRIGGQTKHFLVHETQE
                     EDDDRRKGNMTSEMDGAIAYGHPGKKTPLWLASLIRKETLFLHNILCGAKPEEDYIDL
                     LNGEAAMSAIATADAATLSRSQDRKVKISEIIKHTSVM"
     gene            83356..83481
                     /gene="ythA"
                     /locus_tag="ACHJ69_000509"
     CDS             83356..83481
                     /gene="ythA"
                     /locus_tag="ACHJ69_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YthA"
                     /protein_id="HFO5013575.1"
                     /translation="MIKNFIFDNLIILAVPFMIKTSLKTNLIFFFLCVFVPRMAS"
     gene            complement(83580..83789)
                     /locus_tag="ACHJ69_000510"
     CDS             complement(83580..83789)
                     /locus_tag="ACHJ69_000510"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFO5013576.1"
                     /translation="MVSSSASNVVNCETKQRTQFECIYFSQYWAKGDVIANRAPIGQW
                     EPYSEEPLLGIIVTSVCRINRDAKT"
     gene            83812..84015
                     /locus_tag="ACHJ69_000511"
     CDS             83812..84015
                     /locus_tag="ACHJ69_000511"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5013577.1"
                     /translation="MDITPKYVGIASGLMNAGSAVADIISPIAFGIIIDKTGNWSLPF
                     YGSVALLVIGIFLTFFMRPDKSL"
     gene            84103..84341
                     /locus_tag="ACHJ69_000512"
                     /pseudo
     CDS             84103..84341
                     /locus_tag="ACHJ69_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            84582..85325
                     /locus_tag="ACHJ69_000513"
     CDS             84582..85325
                     /locus_tag="ACHJ69_000513"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO5013578.1"
                     /translation="MKKSSRMSIVHSHAAGVDIGAKFHVVAVPPDADAEPIRTFQSFT
                     GDLHRMSDWLKTCHITTVAMESTGVYWLPAFEILEAAGFNMILVNVSAAKNVPGRKTD
                     VNDAQWLQRRHAFGLLRASFRPVHDISVLRSYLRQRERLTEYRAAHIQHMQKALMQMN
                     VQLHHAVSDITGVTGLSIVRAIVSGEPDPSVLIQYRDVRCKKTPEVLQQALTGNWQPE
                     HLFALEQSVAFFYFYQEKIRERDDQIEIH"
     gene            85480..85719
                     /locus_tag="ACHJ69_000514"
     CDS             85480..85719
                     /locus_tag="ACHJ69_000514"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013579.1"
                     /translation="MALKFVAERGTDMNRWPDASHFTSWLFCPREIRSPVEKCCPRKR
                     DVHPVELLLHSGWQPQRLVGAILRWVLFIVDYHPV"
     gene            85927..86795
                     /locus_tag="ACHJ69_000515"
                     /pseudo
     CDS             85927..86795
                     /locus_tag="ACHJ69_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(87024..88037)
                     /locus_tag="ACHJ69_000516"
     CDS             complement(87024..88037)
                     /locus_tag="ACHJ69_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFO5013580.1"
                     /translation="MSTRINLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHGLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWLELDGDMLRLNGRYEYVMGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTAQ
                     WLAFNQRIMQGEASLSTLSQPEFYDPEIVFFADKLDAYTDQPEFRMISPDGTTFVTRF
                     YSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            complement(88049..89227)
                     /gene="fucP"
                     /locus_tag="ACHJ69_000517"
                     /pseudo
     CDS             complement(88049..89227)
                     /gene="fucP"
                     /locus_tag="ACHJ69_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
     gene            complement(89359..90339)
                     /locus_tag="ACHJ69_000518"
     CDS             complement(89359..90339)
                     /locus_tag="ACHJ69_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="HFO5013581.1"
                     /translation="MSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPF
                     YPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQR
                     DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVS
                     ADAGYQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKSKTAINIEYMKASIRAKVE
                     HPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQWERSH"
     gene            90408..90473
                     /locus_tag="ACHJ69_000519"
                     /pseudo
     CDS             90408..90473
                     /locus_tag="ACHJ69_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858254.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        22265 a        22368 c        23170 g        22670 t
ORIGIN      
        1 cggcagaagt cagcgccata tgccgccaca taaacagctc ggcaacttca cccacatgtt
       61 tgattgccaa tccatgccgt tgcacaaatt cactcaatag cagggcatca cgagtggcgc
      121 gtcggacttt atcggcatgg caaataaatc ccgcatggtc agacattcgt gcctgaattt
      181 tttccctcac aaccgacaac ggcatcccgg ttccgttatc gtgcgcctga gtaatgatct
      241 ctcttataga agccagtgcc ggagctgcca gctgtgcaat atctccatca atattccgct
      301 tttgcgtgca accaataaag cgcgccagac gaacagcaaa aacctgcccg gcattcaatg
      361 ccgcaccgcc aggtcgggtg acgccgtgtg tgccagcaac ttcccccacc gcaaaacaac
      421 cgggcagaga tgtttgtccc cagatatcga cttcaatgcc gccattcatg tgctgattat
      481 tcatggcaaa ctgcaatggc tgcgtgatga gatcgtaacc gtgcatctta tacagcgaga
      541 tagacagagg attcatttgt tgcaggcgtt cgatgggcga tggtgccaga gcgtcgttat
      601 tttccagata cgcgcgaacg tcgtcgtcca gtcgatctaa tgagaacggc aggtcacccg
      661 gcacaggttc tggattacga ttgaaatcca taaatacctg acgaccatgt tgctgctctt
      721 gcgctactgc catatctaac aagctggagc caaaatccat tacccgcgtg gcgtggaacg
      781 gccactgata gcctttacgg aaaatgtttg aagccagctc ctgggtggtg cgatagtaat
      841 ccgcgaggaa gttatactcg ttacccccgg catccacgga atagatgtat gggatcacct
      901 gtacataagt cccggacaaa ttccacggaa acgtgctgcg cggtgtgccg ataccaaact
      961 ggctttcagt cagattggtt agcgtcagac cttcctccag cgccagcccc agcgagccga
     1021 aacatttgtg tggatacaca ctgtcgcgat aaagctcgcc aggcccgcct gttgccagta
     1081 ccacattggg cgcagtcaca attgccagcc cccatgggtt atgggcgcga tgaccggtcg
     1141 cgaggattgc ccccgtcacg cggtcttcgc cgttttcgtc acgctgatgt agcagtttaa
     1201 tcactgttgc actggtcaac attgggatag caagacgctg tacttcttcc agcagcactt
     1261 tcaccattaa ccttgaggta cgcggcccac acgaggttgc acgcccggct tcgtcgtggt
     1321 cggtttgata acgaagaatc gcgccatagc gatcttccgg taattccaga ccaagatatt
     1381 gcaacccgcc aagagtgtgc agagaaccta ccgcttcaac ataagcggtg tcgtgatcca
     1441 tcgccccacc gctcgccagt gcctctgcca gtttggcgaa gttgtcgccg ttgcccgcgg
     1501 tagcggcggt aaacagtgtt tgtttatcag agccagaaca tgccgacgtc cccatatata
     1561 acccggcggt ggcgattaac acattttgct ggcggcgttt aagttccaca gctgcacgta
     1621 gcccggctgc accactgccg actacaaggg catctgtttt ccagagtggg taatcaacac
     1681 cgtctaccgt gacaacccat gcgggttctg cctgtgcaaa accctttggg aaagtaacgt
     1741 gcgtcagtgc atcaaggatg gcctcatcaa gctggctcat gcatcctcca tcggcgtaaa
     1801 caggctgctt tccatcagac gtgggtgctt gataattgga cggaaggcca tgaatggcag
     1861 aatgtcgcgc tcaatctcta cgccaggggc gatctcaatt aattcaacgc cttctgccgt
     1921 taactggaat accgcacgtt cggtgatata cagcacttcc tgaccgcttt gctgtgcgta
     1981 ggcggcgcta aaggtgattt tttccaccag ggcaaccagt ttagggattt gacctgactg
     2041 ggcgatatgc aagccatctg gcgttacatc aatcttgctg cctttggcgt cgaacgtacc
     2101 gcaaaacacc actttacggg cgttttgtgc aatttcgaga aatccgccgg ggccaatgag
     2161 attgccatta agatgggata cgttgacgtt accgtaccga tccatctctc ccatgccgag
     2221 gaaggtgata tcgataccgc cgccactgta gaattcgaat tgatcgagtg atggaataat
     2281 ggcatcggcg ttacgggcgc agccgaacag agcatcatcc agcatcatgc cgttatgcac
     2341 cccctgttct acactcagcc acaaactttg ataaggcacg ccttcgcgca gcgcaacccc
     2401 aggaataccg cccggaatac taaagccaaa gttggttgaa gcacctgcca ccagttcccg
     2461 cgctgcccga cgggcaatca ggcgacgcac cggatgacta accagttcaa tagcgtcgtt
     2521 agaacttaga cggcgatgtt gaccgctgat cgtcaggtcg taaccgccaa gataggtttg
     2581 cggttgttcg cggtgctcaa cgatgccatc gaccagaatt cctggtagct tgacccgacg
     2641 tggttcaatg gtacctgctt ccagcacatc gcggacctgt acgaacactt tgccgccgct
     2701 gttgtgtgct gccagcgcca tcgaatagct atccatatca atcgcttctt cttcgaggct
     2761 aacgttgcct cgtggatcgg cataagtgcc acgcaaaatc gcgtaatcca ctttgaaagg
     2821 gcgataacga agtttggtct caccgtcgat ttcaatcagt tctaccaggt cgtcggttgt
     2881 gcattcattc gacttaccgc cgccattgcg tggatcaaca aacgatccca gcccaacgtg
     2941 ggtaaaaagc cccggacgtc cggcaccgat ctcccgcagc agtgcctgaa ttacgccact
     3001 aggaaagcag taggcttcaa tcgtgttatt ttttaccagc gcctgcattt tgggcgacca
     3061 ggtaaagtgt ccggcaataa tgcgtttgag catttcggcg tgagcaaaac ggttagtgcc
     3121 tttgctgtcg cgatcgccaa tacccagcga gtggattaat gtcagatcgc gcgggtgtcc
     3181 ggtttgcaga aagcgcgctt caatggcggc cagaatatgg tcagcttcca ccatgccgcc
     3241 gccgttaccg ctaatggcaa tggttgcgcc gtcctgaatt tgcgccgcca gtgtctcggc
     3301 ggttgataac ttacgcatac aatttcctta ttgattgtgg aagctcacgc cggctttttc
     3361 ggcaaatgcg tgcgtcgcct gttggcaatc ctgagtggcg gacattaatc cagccagtaa
     3421 ctggtgggag taatcggcgt gtttattttc cagcgccaga atcagttgct taatgtgtgc
     3481 cagtgccacc ggaccacatt tttccagctg cgctaactgg ttcgccatcc agctttccac
     3541 ctgttctttt tctaccactt catcaattaa attcgccgcc tgagcttcct gcgccgtcag
     3601 gcgtttaccc agcatcagca tctggcgacc gaccaccggg ccaacctgat taagcacgcg
     3661 ttcaatgccc atccagccag gcaccatgcc gagcatcact tcaggattag agaattttgc
     3721 gtgggggcga gcgatacgaa tatcacagca caaggcgagt tcaatcccac cgccaatggt
     3781 atgaccgttg agatttgcaa ccgttaattg gggtaaattg cgcaggcgat taaaaacctc
     3841 gttaccgcgt ttaatccatt ttctgcccat atctaatggc gaataggccg accacgattt
     3901 aatatcgccg cccgcacaaa agaatttatc gccggttgcc gttaattcga ctaatcgtaa
     3961 tgtggtgttg ttttcaattt catttaaata atggtcgaga gattcgatca tttcttcatt
     4021 aatagcatga catttatctt cacggttcaa tgtcaggcgg caagaagccg ccgctctgct
     4081 gaacagaacg ggttggtcac tcatgattat tccttagcaa taatgcgcga tgcgatcgac
     4141 gctaatatcc agcgtgctgt taatatcttt ctgccaccag tacggtgaga atatttccag
     4201 ttcaatagcg ccattaaatc cggcgttttc aaccagccga cgaattgacg gaatattgat
     4261 gacaccatct cccggcattc cacggtcatt gaccagatcg gtggtcggca ctaaccagtc
     4321 ggaaacatga aacgcgagga tgcgttttcc ggcacgcagg atctgactgg cgagatccgg
     4381 gtcccaccag acatggtaga catctaccgc cacgccgagg ccaaattcgc catcgggatc
     4441 gagttcatca caccagtcca gcgcctgacg taacgtgcac agacaagaac gattggcagc
     4501 agtcatcggg tgcagtggct ccagtgcaat tggcacgccg acctctttcg aatgtggcag
     4561 caactggcga atcccttgtt tcacctgttc ccgtgcttcg tacagatctt ttgttcccat
     4621 tggcagaccg ccaaccacct gcatataaca ggcggcgttc agtacagcgg cgtcgtccag
     4681 cgcccggcgg ttatcgtcaa tcgccagttt gcgctccgcc agcgtcggtg cggtgtagta
     4741 ggtgctacgg cacaaaccgg aaacgttcat gttactggca gcaagctggc gggcgatttg
     4801 ctggagatct tcactctgca actccctgcg ccagggggca atcgcgccaa taccgcgtgc
     4861 ggcgcaggca tcaataatgg cgggtagcgg cgttttatag cccagtgtcg cggtattgat
     4921 actgaataaa tccgggcgtt gtaacagatc tctcatgatt cagcctcaga agcgcggcat
     4981 acttaagccg ccgtcgatgg cgaccgcctg accacaggtg taaataagtt taccttccgc
     5041 catcgcgcgg acagtggaag caatatccgc cggataaccc caacgtcccc acggcaccag
     5101 acctttggca atcagttcgt cgtaataggc cgttgccgga atagtcatat cggttttgat
     5161 gagaccagga cgcacttcat atacgccaat ttgttcatta caaaggcgag cagcaaacag
     5221 acgagcagcg gcagagacgg cagttttggc aatggtatat tcgccgcgat tcatcgccag
     5281 catgatggcg ttgatggaac tgacaaaaat aatcgagcgg tgcggcagtt ctgcttccgg
     5341 tttcggctgt gccagtaagc gcttactgaa tgcctgagca aggaagaacg gtgcgcgggt
     5401 gttaatggca atattttggt cgaagctgtc tggttcgagg tcgagaaggt caccgcgttt
     5461 tttcactgaa atgcctgcgt tgttgagcag gcagtccaga cgtccccact ggttttgcgc
     5521 tgcgtcgagc atctcttcat gcagggagag atcgcccaca tcagcaggga agcaaatcac
     5581 ttccacgcct tccgccagac attcttgttg tgtggcatgt aatttttcta cgctatccgc
     5641 atccggacga tcattaatca gcagattaaa tccgccacgc gcaagctcca gcgcacagcc
     5701 ctttccaata ccacgagcgg ctccggtaat cacggcaaca ggacgagtgg atttcataca
     5761 atagctctct ttttttgaat tttgtttcct tagtgatgat tagcttgcga ctggaggata
     5821 aaaagatcaa ccatctagtt ggttgatatg cgatggcgat cacaggacat ttgataacct
     5881 gatgaaagta caagtttttg cccttttttc tgaagctgaa atcaaatcag gttatgatag
     5941 tcaccacgaa gaggtcgtgg ggaagcgtta tgtatcttga taccagcaac gtacagagcc
     6001 tgaaagaaaa gctgctgtta tgcgcggtta atgaatttgc cgaatatggt tacgaagggg
     6061 ccagagtcga taatatcgtc aaggccgcgg ggtgcagtaa acagacggtt tatcaccact
     6121 ttggtaataa agaaaattta tttatcgaag tactggaata tacctggaac gatattcgac
     6181 aaaaagaaaa ggcccttgat ttctcagatc ttccgccaca gaaagccatt gagaaaataa
     6241 tcgattttac atgggattat tatatttcta atccctggtt tttaaaaatt gtgcatagtg
     6301 aaaatcaaag taagggtgtg cattatgcaa agtcacagcg tcttttagaa attaaccatg
     6361 cgcacttgca gttaatggaa tccttattgg atgaagggaa gaaaaacaat atttttaagc
     6421 cagatatcga cccattgcag gtgaatatta atattgctgc gcttggggga tattatttga
     6481 tcaaccagca tacgcttggt ctggtttatc acatcagtat ggtttcaccg caggcactgg
     6541 aagccagacg taaggtcatc aaagagacga tccttagctg gcttttggtt gacccttcat
     6601 ctaccgcgcg cgaataaaac tgccatccgg ctggcgggta aacaggacct gttgattatt
     6661 ccccgtatca atggttaagc ccgtcaccac gccgctggcg ttctggcgga ttttcaccat
     6721 ctgcccgttt tgcaaattac tcagtggctt atccgcgcct tctacctgcg ccatggcata
     6781 gacatcggta gcgggcagtc cgtggtcgcg gaacagctgc gccatcgttt taccgggttc
     6841 gacacgatag gagcgccatt gattatcaat tccgctatct ggctggaatg gttgcgtatg
     6901 ggtggtttgc ggctgctctt ctggttggcc ttcctgtatt ggttctggag cgaccggagc
     6961 cacctgatca ggatcatttt gtggcgtgac taattgcgcc cgcagctgtt cttctgtcgg
     7021 tggttgtgat tgcgactgaa tatctaactg cgcttcacgc gtcaccaccg gcgcgttggg
     7081 cgtatcatca gatggaagca gaaatccgac caccaataca atggcggcaa taatgatccc
     7141 ccgacgatgc atcggcggca gcgggtccat gaagcgaaaa ttgtccggcg cgtgccagac
     7201 tttctccagg gttggtttta gttcaaagcg cccgggcatg gtttcctcct gctccgcgtc
     7261 ttgttcctca atcatagcct atgaataagc taacgctatg atgtccgtgg taaacccgcc
     7321 tttattatat tcatacggga tattgctatt gtttcttttt ccccgggatt tgtcatcatt
     7381 cccgcgacaa agttttaccc aaagaagtgt ggctgatatg ctgcccgcta ctttataccc
     7441 taagaaagga aatacgatga ccaccccaac ttttgacacc atcgaagcgc aagcaagcta
     7501 cggcattggt ttgcaggtag ggcaacaact gagtgaatct ggcctggaag ggctgctgcc
     7561 agaagcactg gttgcaggca ttgccgatgc gctggaaggc aaacatccgg ctgttccggt
     7621 tgatgtggtg caccgcgcgc tgcgtgaaat ccacgaacgc gccgatgccg ttcgtcgtca
     7681 gcgtttccag acgatggctg ctgaaggtgt gaaatacctg gaagaaaacg ccaaaaaaga
     7741 aggtgtaaat agcaccgaat ctggcctgca attccgcgtg atcaaccagg gcgaaggcgc
     7801 aattccggca cgtaccgacc gcgttcgtgt tcattacacc ggaaaactga tcgacggcac
     7861 cgtgtttgac agctccgttg ctcgcggcga gccagctgaa ttcccggtta acggcgtgat
     7921 ccctggctgg attgaagcac tgactctgat gccggtaggt tctaaatggg aactgactat
     7981 cccgcaggaa ctggcatatg gcgagcgtgg cgcaggcgcg tccatccctc cgttcagcac
     8041 cctggtgttt gaagtcgaac tgctggaaat cctctaagca gcgcattctg ttcccctcga
     8101 accagagggg agcaggcatt cagcaataaa cccttcagtt tgccaaacgg cgctatttta
     8161 tgttgcaaag accctgtaag cgtgtatttt tgtgagctgt ttcgcgttat caccgtgata
     8221 tgacactcac tttaaacata aaattaacat tagatctaaa tcttagtatt catcccgcgt
     8281 attgttacct aatatcgatg agtcccgata cagattcgtc gtatcataga ctgactaaag
     8341 gccgtagagc ctgaacaaca cagacaggta caggaagaaa aaaacatggt agatcaggta
     8401 aaagtcgttg ccgatgatca gtctccggct gaacagtcgc tacggcgcaa tctcacaaac
     8461 cgacatattc agcttattgc cattggcggt gccattggta cagggttgtt tatggggtcc
     8521 ggcaaaacga ttaaccttgc cgggccgtcg atcattttcg tttatatgat catcggtttt
     8581 atgctctttt tcgtgatgcg ggcaatgggg gaattgctgc tttcgaatct ggaatacaaa
     8641 tcttttagtg acttcgcttc cgatttactc gggccgtggg caggatattt caccggctgg
     8701 acttactggt tctgctgggt tgtaaccggt atggcagacg tggttgccat taccgcctat
     8761 gcgcaattct ggttccctgg gctttctgac tgggttgctt cgttatccgt gatcattctg
     8821 ttactggttc taaacctcgc cacggtaaaa atgttcggtg agatggagtt ctggtttgcg
     8881 atgatcaaaa tcgtcgccat agtgtcgctg attgttgtcg gcctggtcat ggtggcgatg
     8941 cactttcagt caccgaccgg tgtggaagcg tcatttgcac atttgtggaa tgacggcggc
     9001 tggttcccga aaggcttaag tggcttcttt gctggattcc agatagcggt tttcgctttc
     9061 gtggggattg agctggtagg tacaacagct gcggaaacca aagatccaga gaaatcactg
     9121 ccacgcgcga ttaactccat tccgatccgt atcattatgt tctacgtctt ctcgctgatt
     9181 gtgattatgt ccgtgacgcc gtggagttcg gtagtcccgg agaaaagccc gttcgttgaa
     9241 ctgtttgtgt tggtaggttt gcctgcggct gccagcgtga tcaactttgt ggtgctgacc
     9301 tctgcggcgt cttccgctaa cagcggtgtc ttctctacca gccgtatgct gtttggcctg
     9361 gcccaggaag gtgtggcacc gaaagcgttc gctaaactct ctaagcgcgc agtacccgcg
     9421 aaagggctga ccttctcttg tatctgtctg ctcggcggcg tggtgatgtt gtatgtgaat
     9481 cccagcgtga ttggcgcgtt cacgatgatt acaaccgttt ccgcgattct gtttatgttt
     9541 gtctggacga ttatcctttg ctcgtacctg gtgtatcgca aacagcgtcc tcatctgcat
     9601 gagaagtcga tctacaagat gccactcggc aagctgatgt gctgggtatg tatggcgttc
     9661 tttgtgtttg ttctggtgtt gttgacactg gaagatgaca cccgccaggc gctgctggtt
     9721 accccgctgt ggtttatcgc gctggggctg ggctggctgt ttattggtaa gaaacgggct
     9781 gctgaactgc ggaaataatc gcattatcat gctggatggc gcaatgccat ccagctttta
     9841 gatcactcac ccgccagcgc gcgcgggaat agtacattgt tttccagact gatgtgctcc
     9901 atcaggtcat caatcagttc attaatgccg ttatacatcg ctttccaggt ggtgcaggct
     9961 tccggcggtg gtgtgacgtt attggtggtg tgtttgatca cttccagcag ttcgcccgct
    10021 tcatcgtgct cgctttccat tacgctgatt ggccccattg cctggctgcc catgccttgt
    10081 ttaatcatcg ggaagagaat ttgctcttct ttcatcatgt ggctggaaag ctcttcatgc
    10141 agcatagtca ggtattttgt cagccctttt ggcacacttg gtttgtcggc gtgaacgcgc
    10201 tcgactttag tcgcttgcag gatcagctcc ggcagctgct cgcgatggcg atcgtgatag
    10261 cgcacaataa tgtggtcgat gatttccgcc agcggggcgc tacgccagtc tttctcaatc
    10321 ggttgttcag caagctttgc cagttcggct tcaatgacgt caacatccag ttctttacgt
    10381 gccgccgcgc gcgccagcgt ctgcttaccg ccacagcagt aatccatatc atatttacga
    10441 aacagagccg aagcgcgagg aatagagagc gccagttcac ctaaaggttg gtcgcgataa
    10501 gccatagctg atacctcatt cttaataaga taagatgcat tttaaatgca tctttaaggc
    10561 aaaagctata acccttacgt agcagaggct tatttaactc actgccagcg tgtcaacttt
    10621 ctgagacaga ttcagttaaa ggtttttctg gttttgctct gaccgcgatg accacgccaa
    10681 tcactaacag cgcgataccg ctcagcgtca ttagcggcgg catttgctgg cggagtaaaa
    10741 aggtgtacag caaacctgcc agcgtttcga aaacaatcag cggcccgaga atcactgtcg
    10801 gtaatcgctg gctggcgacg ttccagcaga gtgcgccaac ccatgagcaa agcacggcta
    10861 tcgcaaccat cagactaata aacaccagcg gacgggggcc aaagggtaag gagaagtccg
    10921 gcgtttgcat attcagccag taacaggcga cgagatagcc gatgagagaa accggcagcg
    10981 tgaccagcgc ctgcgccgtc gcccacatca tcggatgttt gtcgggattt tcccgcagcc
    11041 agcgggcgtt gcgcagggca taccatgccc agcagaccac ggaaactaac gctagcacga
    11101 tgcctgaggt ataacgtgcc cagtcaaaat cggggagtcc gtggtttaac tcagcaatat
    11161 tcacacacgc caggccgatg ccaatacaaa ccagtgccgg ggcgagtttt ccccatgcga
    11221 gtttgccgtc gcgctggcta taaagcagat tggcaaaaac gggaatcacc accggcaggg
    11281 tgccgataat catcgtggaa acaggcgcgc cagtacgttg aatggcactt gcaaggcaga
    11341 agtaatagat aaggttgccc atcatggtga gcatcaaggc ggtaagccag tcgcgacgcg
    11401 ccaactgacg cagacgcacg cgtcccagcc aggcaatggg cagcgcaatt aaccctaacg
    11461 ccagataacg ccccatcgac tgcaacatcg ccgggtattc cggtacgatc aacggcccga
    11521 caaaaataag cccccacatc aaccctgcta acagggcgta cagcacgccg ctaatcatta
    11581 ctggcatcca ttggtctgtc agaagaaagt tcaggaagca gacatgattg ctcccggcat
    11641 cccgtcatta tcagagagga tgcgggagga tgccacttta attaactagt tattaacatt
    11701 aaaaagatgg cttacgcttt cggctaacgt tgtcgttggg cggccaatca atttgctaag
    11761 cgttttgctg tcatcaaaca gaccgccttt cgatgcgcca acgtcagaat ccgccagcat
    11821 atccgccagt ccatcaggca gaccgacgct tttcagcgcc gcggcgaaat cagcttcgct
    11881 caggttttga taggtaatcg gtttgccgct ctgtttggtg agttctgccg ctaactgtgt
    11941 cagcgtccag gcactatcgc ccgccagttc gtaaaccttg ccttcgtgac cggcttcgct
    12001 aatcacgcgt gccgcagcta ccgcataatc agcccgcgtt gctgaggcaa ttttgccatc
    12061 gcccgcggca ccgataaata cgccgtgttc cagtgctgcc ggggcgctgg cgaggtagtt
    12121 ttcactgtac cagccgttgc gcagcagggt gtaaacgatg ccagaatcag ccagcatttt
    12181 ctccgtctcg atgtgctcat cggcgaggcc gagcggggag gaatcggcat gtagcaggct
    12241 ggtataagcg ataaatttca cgtcggccgc ctttgcggca ttaataacat tacgatgctg
    12301 cggggcacgt tgacccactt cgctggaaga gatcagcagt agtttttcca ctccctgaag
    12361 cgcagatgtc agtgctgctt catcgccgta gtcagcctga cgcacggtaa tgccttgtgc
    12421 tgtcagtgcc tgtgcttttg ccggattacg aacgatagcc actatttggc tggcaggaac
    12481 cgttttcatc aaggattcaa taacatagtg accaagttgg ccagtggcac cagtaatagc
    12541 gatcatggga agtctccatc gttttgtctt gtgtttaaat acgctaacac ctaaacttac
    12601 ttttagtaag tacgtacaaa aaggtaagta tgaaatgagt caggttagcc tgtcgcaaca
    12661 actgaaagag ggaaatctct ttgcggaaca gtgcccgtcg cgcgaggttt tgaaacacgt
    12721 caccagccgt tggggggtgt tgattctggt ggcgctacgc gaaggtactc atcgctttag
    12781 cgacctgcgg cgcaaaatgg gcggggtgag tgaaaagatg cttgcgcagt cgttacaggc
    12841 attagaacag gatggttttc ttaaccgtat cgcgtatccg gtggtgccgc cgcatgtgga
    12901 atatagcctc acgccgctgg gcgagcaggt gagcgaaaag gttgctgcac tggcggactg
    12961 gattgagttg aatttgcccg aggtgttagc ggtgcgggat gaacgtgcgg cataactgcc
    13021 ggatgcgctg cgcttatccg gactacggtt catggcttgt agggcggata agatgcgtca
    13081 tcatcgcatc cgccaataaa tgccggatgc ggttgatgat ttgtaggccg aaaaagaagt
    13141 gttttactta ctcagatcca cctgataaat cgcaaacccg atatcatcgg tcgcgacttt
    13201 attcatcgga tactgccctt tctctttaat aaacgctgcg gctttatctg acggagaggt
    13261 ttcgaaacgg atatccagtt tcttatcgcc agctatcggt gctaaacgcc agttgttatc
    13321 tgccgccggg tgaatttccc ccgcacgttt cgactcatca gcaatccacg ctgccagcac
    13381 cgagcggttc tcatccggtg aagcaaaagc gatatggctg tcgcccgtac cggcaaattt
    13441 gccgccgtaa gcgcgatagt tattggtggc aacgaggaac atggcgttcg gatcaatcgg
    13501 cttgccgtta aaggtcaggt tcttaatcct ttccgcattg gcattaatca tctggcactc
    13561 gccgtcataa cgggcgggct gggtaacatc aatctgataa ttcacaccat caataacatc
    13621 aaagttatag gtgcggaaac catcccagtt gatgagtgac tgtggtttcg tgctgttagg
    13681 atcaatctgg ttaaactgtc cggcggagca ctccagccac tctttcacct ctttaccgct
    13741 ggctttcacc acaatcagcg tattggggta gagataaaga tcggcggcat tacggaaggt
    13801 caactggcct ttttccacct ccacatagct tgccgggtca tttttgcgac caccaacttt
    13861 aaacggtgcg gcagctgaaa gcactggcag ttttgccaga tccggatcgc cctgagtgta
    13921 atgctcgaca tacgcttttt gcgcgttgtt caccacctgc acggtcggat cgtcctgcac
    13981 aagcgccaga tagctataca tattgtcggc agatttaccg attggcttgc tgacgaactg
    14041 gcgtgtggca tcgtgatcgg ctttgagtgt ttctaccagc ttgctgtctt ccgccgcgag
    14101 ggatttttta ttagcgatgt cgtaaatcgg ccgtgcttcc gctttcgcct gcgtcacctg
    14161 ccatttaccg ctgtcattac tgagttgtaa gtcgaccaca ccaagatgat cgccccacat
    14221 gcctggcatt accgccggaa caccattcag cgtgcctttc gcgatatcag ccccttcgat
    14281 atcagcaaaa tctttacctg ggaaaacggc gtgagcatgg ccaaacataa tggcgttaac
    14341 gcccggaatt tcactgaggt aataaactga gttttccgcc atcactttat acggatcggc
    14401 agacagcccg gaatgcgcca gaacgacaac gacatcggca cctttctcgc gcatttcagg
    14461 cacgtatttg cgcacggttt cggtaatatc attcaccgtc actttcccgg ataaattagc
    14521 tttatcccag cccatgattt gtggcgggac gacgccaata taaccaatct tcagcgtctg
    14581 tttttttccg tctttatcga ccacttcggt gtctttaatt aaatacggtg taaacattgg
    14641 ctgtttggtt ctggcgtcaa tgacgttggc atttacataa gggaatttcg ctcccgccag
    14701 cgcatttttc agataatcca gaccgtagtt aaactcgtgg ttgccaagcg tgccgacggt
    14761 atagtccagc gtatttaatg ccttatagac cgggtgaata tcacctgctt ttaatccttt
    14821 cgccgacatg taatctgcca gcggactccc ctgaatcaaa tcgccgttat cgaccagtac
    14881 gctgtttttc acttcattgc gggcatcgtt aatcaggctt gccgtacgta ccagtccgaa
    14941 tttttccgtg gcggtgtctt tgtaataatc gaaatccatc atgttgctat gcagatcagt
    15001 ggtttccatg atacgcagat cgaccgtcgc tgcattcaca ctggcggcaa tcagcgtggc
    15061 caggagcgtt gcgctaaact taatcatcag ggacatcctt ttatcatcgg gaatacgaaa
    15121 gaaaagggag aataaacgtc ttacttatag aacagtgaag aatgccacaa ttttacgctt
    15181 tgaaaatgat gacactatca cagttggcgc attcattaac gatagggtat aagtaaaaca
    15241 ataagttaac accgctcaca gagacgaggt ggagaaatgt tagatcaagt atgccagctt
    15301 gcacggaatg caggcgatgc cattatgcag gtctacgacg ggacgaaacc gatggacgtc
    15361 gtcagcaaag cggacaattc tccggtaacg gcagcggata ttgccgctca caccgttatc
    15421 atggacggtt tacgtacgct gacaccggat attccggtcc tttctgaaga agatcctccc
    15481 ggttgggaag tccgtcagca ctggcagtgt tactggctgg tagacccgct ggatggtact
    15541 aaagagttta ttaaacgtaa tggcgaattc accgttaaca ttgcgctcat cgaccacggt
    15601 aaaccgattt taggtgtggt gtatgcgccg gtaatgaacg taatgtacag cgcggcagaa
    15661 ggcaaagcct ggaaagaaga gtgcggtgtg cgcaagcaga ttcaggtccg cgatgcgcgc
    15721 ccgccgctgg tggtgatcag ccgttcccat gcagatgcgg agctgaaaga gtatctgcaa
    15781 cagcttggcg aacatcagac cacgtccatc ggctcttcgc tgaaattctg cctggtggcg
    15841 gaaggacagg cgcagctgta cccgcgtttc gggccaacga atatttggga taccgccgct
    15901 ggtcatgctg tggctgcagc tgccggagcg cacgttcacg actggcaggg taaaccgctg
    15961 gattacactc cgcgcgagtc gttcctgaat ccggggttca gagtgtctat ttattaatta
    16021 atttatgcag caggtccatc acttgctgca cctcttcctg agtaagcgcc ccgtctttgg
    16081 cccattgcac gcgaccgtct ttatccagca ccactacagc ggaactttct tcatccagct
    16141 gccaggcacc gcgtgcgacg ccattgctat caacaataaa ctgcgaccag ggataaagct
    16201 ttttattact ctccagactg ctgcgcacaa acatccctga acccggaatc gcgtcgtcgg
    16261 tgttaacaat ggtggtggtc tggtaacgat cgtgcggtaa cttcgctgat ttaatcgctt
    16321 caatcagcgt cgcgtttttc tcttttgcag aggtgcgacc agcaatatgt tgcagtactc
    16381 gcacttttcc cactaactgc gcgctgttcc aggttttgta gctaaactga tctttatcaa
    16441 gcaccaactc gccacgatcg gtgatgccaa tcggcggcac tcgctgaccg gtttcgaact
    16501 gatgtgcgga agccatcatc ggcaacagca ggcaggtgag cgccagaatc ttgcttaggg
    16561 tcatggtgtt tccttcttat gatatgcagg tgatccgacc acttgggtct gagttttaat
    16621 catatgtgct atttatcgaa tttcccgcaa gtgtgatgcc agtttgcggt caagcgcaca
    16681 aatcatatga aaaatgaatg cttatactga agaccgcgct tcggtaaaaa gataattctg
    16741 aataattgta acctttaggt aaaaaaagtt atacgcggtg gaaacattgc ccggatagtc
    16801 tatagtcact aggcattaaa atttgcgcct cataatagtt gggctgattg tggcaccgca
    16861 aaggcgtaat actcagcagg agataacaat gaaaattttc caacggtaca acccacttca
    16921 ggtggcgaag tacgtaaaga tcctgttccg tggacggtta tacatcaagg acgttggcgc
    16981 ttttgaattt gacaagggta agattcttat cccaaaagtg aaggataagc tgcatttgtc
    17041 agtgatgtcc gaagttaacc gtcaggttat gcgtctgcaa acagagatgg cttaaccaaa
    17101 gtgctatgca gtaaaaagtg ctatgcagta ataagacggc tcctgattca ggagccgttg
    17161 atgtttctgg ggttatgcga cgctttcttc tttatcttta gcgtcaggca gttttggcga
    17221 aagggcggtg gctttgctgt cgattcgggt caccagcagc tggtcgatgc ggtagttatc
    17281 gatatccacc acttcaaatt tgtagccggc gaatttcacc gaatcggtgc gtttcgggat
    17341 cttacgcagc ataaacatca taaagccgcc gatggtttcg tagttgcccg actgcgggaa
    17401 ctcgtcaata tccagcacgc gcatgacgtc gtcaatcgga gtgccgccat ctatcagcca
    17461 tgagttctca tcacgggcga caatctgctc ttccagcccc tgaccgacca gatcgcccat
    17521 cagcgtggtc atcacgtcgt tgagggtgat gatccccact accagcgcgt actcgttcat
    17581 gatcaccgcg aagtcttcac ctgcggtttt aaaactttcc aacgcctctg aaagggttaa
    17641 cgtatccggc acaatcagcg tattgcgaat ttgtacgccg ctgttcagtg ccaggctttg
    17701 gttagccagc acgcggttca gcaggtcttt agaatcgaca tagccgatga tgtggtcaat
    17761 atcttcatta cagacgagaa acttagagtg cggatgttcc gccaccttat tcttcagact
    17821 ttgctcatct tcgtggagat caaaccaaat cacgttttca cgcggtgtca ttgaagacgg
    17881 aacggtacgg gattccagct caaagacgtt ttcaatcagc tcgtgttcct gtttacgtaa
    17941 cacgcccgcc agcgcaccgg cttccactac cgcgtagata tcatcagaag tgatgtcatc
    18001 tttacgtacc attggcagtt tgaaaatacg gaagattatg ttcgccagtc cgttgaagaa
    18061 ccacaccagc ggggtgcaaa cgtacaggca gaagcgcatc gggttgatga tacgcaaagc
    18121 cacagcttct ggcgcaatca taccgatgcg tttcggggtt aaatccgcaa acaggataaa
    18181 catgccagtc actaacgaga aagagagaat aaagctcagt tgctcagaga gttctgccga
    18241 catatagcgg gagaacaggc tgtgaaaagc tggagaaaat gccgcatcac cgacgatacc
    18301 gccgagaatc gccactgcgt tcagaccgat ctggaccacg gtaaagaaca tgccgggatt
    18361 ttcctgcata ttcaaaacgc gttgggcatt tatattgcct tcatcagcca gcagtttaag
    18421 tttgattttg cgtgaggcgg caagcgagat ctcggacatc gagaagaacg cacttacagc
    18481 gatcaaacag agtatgacta aaatactgtt taacatatct tatccgacct ttcaggccag
    18541 atcctcggaa ggggaagttg attaatttgt gtgaaataca cattgaaagc cgattcgaag
    18601 aaagtgaacc ggcattttca gtggctagta tagcgtaagg tactgtaaag ccgccagagg
    18661 gttaaaattc agacagctga aaaatgaaaa actgcctgat gcgctatgcc gaacaggcct
    18721 acgcagtccc cgcaatatat tgaatttgga cgattttgta ggcctggcaa ggcgtttacg
    18781 ccgtatccgg cataaacaaa atgcacttta tcaacaatct acatttgtac ccgtaacgct
    18841 atgcttccgg cggcagacag acgccaattc cgccaattcc acagtaacca tacgggtttt
    18901 tatgcagata ttgctggtgg tcatcttcgg cataataaaa cggtgtggcg ttagcgattt
    18961 ccgtggtgat gcgacgatcg tcatcggcgg caagcatcgc cgcctgaaaa cgttccagac
    19021 tggcgcgagc ggcggcatcc tgttctgggg tcagcggata aatcgctgaa cgatactgcg
    19081 tgccgtggtc attgccctga cgcattccct gggcgggatc gtgattctcc caaaatactt
    19141 gtagcaactg ctcatagctg atgacggatg gatcgtaaac aatgcgcacc gcttcggcat
    19201 ggccagtatc gccggagcac acttcccgat aagtcggatt tggcgtatag ccgccggtat
    19261 agcctgcggc ggtgctgtaa acgccgggta actgccagaa cagacgctcc acaccccaaa
    19321 aacaacccat cgcaaaaatg gcaatctcca ttccgtcagg tacattggtc attgagtgac
    19381 cgttgaccgc atgcagtgtg gctacgggca tcggggtgtt acgtccaggc agggcatcgg
    19441 cgggggaaac cagatgcttt ttatcaaata aactcatggt gtcgctctcc cgaaaattgg
    19501 tcattggggt taaggttgta acaagaggcg tatttgcaca caataaccac cgtgaatagg
    19561 tctaaagtaa aacataagaa atattttggg tttagtctgc tttttaatcc atattactgg
    19621 atttttgtta agccgtttaa cggcgttcca ggggcaggaa aaaaggatat tcaggagaaa
    19681 atgtgcgcta tatccgacag ttatgctgtg taagcttact ctgcttaagc ggatctgccg
    19741 tcgccgcgaa cgtccgtcta caggtcgagg ggttatcggg agagctggaa aagaacgttc
    19801 gcgcgcagct ttctacgatt gaaagtgatg aagtgacgcc agaccgtcgc tttcgcgcac
    19861 gcgtcgatga tgccatccgc gaaggtctga aagcgctggg ctattaccag ccgaccattg
    19921 aatttgatct ccgtccaccg ccaaagaaag ggcggcaggt attgatcgcc aaagtcacgc
    19981 caggcgtgcc ggtgttaatt ggcggcaccg atgtggtatt gcgcggcggc gcgcggaccg
    20041 ataaagacta tttgaaattg ctcgatactc gcccggctat tggtacggta ctgaaccagg
    20101 gcgattatga aaatttcaaa aagtccttaa ccagcattgc gttgcgtaaa ggttatttcg
    20161 atagcgaatt taccaaagcg cagctgggca ttgcgctcgg cctgcataaa gccttctggg
    20221 atattgatta taacagtggc gaacgttacc gctttgggca tgtgaccttt gaaggatcac
    20281 aaatccgcga tgaatacctg caaaatctgg tgccgtttaa agagggcgat gagtacgaat
    20341 cgaaagatct ggcagaactg aaccgtcgac tttctgctac cggctggttt aactcggtgg
    20401 tggtggctcc acaatttgat aaagcgcgcg aaacgaaagt attaccattg acgggcgtgg
    20461 tttcgccgcg aacagaaaac accatcgaaa ccggcgtcgg ttactctacg gacgtgggac
    20521 cgcgcgtgaa agcgacgtgg aaaaagccgt ggatgaactc ttacggtcac agtttgacca
    20581 ccagtaccag tatttccgcg ccggaacaga tcctcgactt cagctataaa atgccgctgc
    20641 tgaagaatcc gctggaacaa tattatttgg tgcagggcgg ttttaagcgc actgacctga
    20701 acgataccga gtctgactcc actacgctgg tggcttctcg ctactgggat ctctccagcg
    20761 gctggcagcg tgccattaac ctgcgctgga gtttcgacca ctttacccag ggtgaaatta
    20821 ccaacaccac gatgctgttt tatcctgggg tgatgattag ccgcacgcgt tctcgtggtg
    20881 gcctgatgcc aacctggggc gactcgcaac gctactctat cgactactcc aacacggcct
    20941 ggggctcgga tgttgatttc tccgttttcc aggcgcagaa cgtctggatc cgcacactgt
    21001 acgatcgcca tcgttttgtg acacgcggca cgctgggctg gattgaaacc ggtgatttcg
    21061 acaaagtgcc gccggatctg cgtttcttcg ccgggggcga tcgcagtatt cgcggctaca
    21121 aatacaaatc tatcgccccg aaatacgcca acggtgacct gaaaggggcc tcgaagctga
    21181 taaccggatc gctggaatac cagtacaacg tgaccggaaa atggtggggc gcggtgtttg
    21241 tcgatagcgg cgaagcggta agcgatattc gccgcagcga ctttaaaacc ggtaccggtg
    21301 tcggcgtgcg ctgggaatcg ccggtcgggc caatcaaact cgattttgcc gtgccggtcg
    21361 cggataaaga cgaacacggg ttacagtttt acatcggtct ggggccagaa ttatgagttt
    21421 atggaaaaaa atcagcctcg gcgtggttat cgttatctta ctgttgctgg gatcggtggc
    21481 gtttctggtg ggcaccacca gcggcctgca tctggtattt aaagcggcgg atcgctgggt
    21541 gccaggactg gatattggca aggtcaccgg cggctggcgt gatctcacct tgtctgacgt
    21601 tcgttatgag cagccaggcg tggcggtaaa agcgggcaat ctgcatctgg cggtcgggct
    21661 tgagtgcctg tggaacagta gcgtttgtat taatgatctg gcgctgaaag acattcaggt
    21721 caacatcgac agtaaaaaaa tgcctccttc tgaacaggtt gaagaagagg aagatagcgg
    21781 tccgctggat ctctccacgc cgtatcccat taccctgaca cgggtggcgc tggacaacgt
    21841 caacgtcaag attgatgaca ccacggtatc ggtgatggac ttcacctccg gcctgaactg
    21901 gcaggagaaa accctgaccc tgaaaccgac atcgctgaaa ggcctgttga ttgctttgcc
    21961 gaaagtggcg gaagcggcgc aggaagaagt ggtcgaaccg aagattgaaa atccgcagcc
    22021 ggaggaaaag ccgctcggcg aaacgctgaa agatctcttt tctcgcccgg tattgccgga
    22081 aatgaccgat gtgcatttgc cgcttaacct gaacattgaa gagtttaagg gcgagcagct
    22141 gcgcgtgacg ggcgacacgg acatcaccgt gagcaccatg ctgctgaaag tgagcagcat
    22201 tgacggcaat actaaactgg acgccctgga tatcgactcc agtcaaggga tcgtcaacgc
    22261 cagcggcacg gcgcagctgt cagacaactg gccggtggat atcaccctca acagcacact
    22321 gaacgtggag ccgttgaaag gtgaaaaggt gaagctgaaa gtgggcggcg cgctgcgcga
    22381 acagctggag ataggcgtta acctttccgg tccggtggat atggatttac gcgcccagac
    22441 gcgactggcg gaagccggat tgccgctcaa cgtggaagtg aacagcaaac agctttactg
    22501 gccgttcact ggtgagaagc agtatcaggc ggatgatctg aaactgaaac ttaccggcaa
    22561 aatgaccgat tacgcgatct ctatgcgcac ggctgttaag ggccaggaga tcccgccagc
    22621 caccattacc cttgatgcca aaggtaatga acagcaggtc aatctcgaca aacttaccgt
    22681 cgcggcgctg gaagggaaaa cggaacttaa ggcgttgctc gactggcagc aggccattag
    22741 ttggcgcggt gagctaacgc ttaacggcat taacaccgcc aaagagatcc cggagtggcc
    22801 gtcgaaactc aatggcttga ttaaaacccg cggtagcctg tacggcggca cctggcagat
    22861 ggaggtgcca gaactgaagc tgaccggtaa cgtcaaacag aacaaagtga acgttgacgg
    22921 cacgctgaaa ggcaacagtt atatgcagtg gatgatccca gggctgcatc tggaactggg
    22981 gccaaacagt gccgaagtga aaggcgagct gggggtaaaa gatctcaatc ttgatgccac
    23041 catcaacgcg ccggggctgg ataacgcgct gccggggctt ggcggtaccg cgaaagggct
    23101 ggtgaaagta cgcggcacgg tggaagcgcc acaactactg gcagatatca ccgcgcgcgg
    23161 cctgcgctgg caggaacttt ccgtggcgca ggttcgcgtg gaaggcgaca ttaaatccac
    23221 cgatcagatc gccgggaaac tcgacgtacg cgttgagcaa atttcgcagc cggatgtaaa
    23281 tatcaacctc gtcaccctga atgccaaagg cagtgaaaag cagcacgagc tacagttgcg
    23341 gattcagggc gagcctgtct ccgggcagct taatctggca ggaagttttg atcgcaaaga
    23401 agaacgctgg aagggaactc ttagcaatac ccgcttccag acgccggtcg gcccgtggtc
    23461 gctgacccgc gatattgcgc tggattaccg caataaggag caaaaaatca gcatcgggcc
    23521 acactgctgg cttaacccga atgcggaact gtgcgtgccg caaactatcg atgcgggtgc
    23581 cgaagggcgt gcggtggtga atctcaaccg cttcgacctg gccatgctga aaccatttat
    23641 gccagaaacc actcaggcca gcggtatctt cacgggtaaa gcggacgttg cctgggacac
    23701 cacgaaagag gggctgccgc agggcagtat cactctttcg gggcgtaatg tgcaggtgac
    23761 gcaaaccgtc aacgatgcgg cgctgcccgt ggcgtttcag acgctgaatc tgacggcgga
    23821 gttgcgtaac aaccgtgccg aattgggctg gaccatccgc ctgaccaata acggccagtt
    23881 tgatggtcag gtgcaggtga ccgatccgca aggccgccgt aatcttggtg gcaacgtcaa
    23941 tatccgtaac ttcaaccttg cgatgataaa ccccatcttt acccgtgggg aaaaagcagc
    24001 ggggatggtg agtgccaact tgcgtctggg tggtgatgtg caaagtccgc agttgtttgg
    24061 tcagcttcag gttacggggg tggatatcga cggcaacttt atgccgtttg atatgcaacc
    24121 gagccagctt gcggtcaact ttaacggtat gcgctcgacg cttgcaggta ccgtgcggac
    24181 ccagcagggt gaaatctacc tgaacggtga tgccgactgg agccagattg aaaactggcg
    24241 ggcgcgggta acagcgaagg gcagtaaagt gcgtatcacc gtgccgccga tggtacgaat
    24301 ggatgtatcg ccagatgttg tattcgaggc tacaccaaac ctgtttaccc tcgatggtcg
    24361 cgtggatgtt ccgtgggcgc gcatcgtggt gcacgatctg ccggaaagcg cagtaggcgt
    24421 ttccagcgat gtggtgatgc ttaacgataa cctgcaaccg gaagagccga aaacggcgtc
    24481 gattccgatt aacagtaacc tgactgtcca cgttggcgac aatgtgcgca ttgacgcctt
    24541 tggcctgaaa gcgcggctga cgggcgatct caacgtcgtt caggacaaac aagggctggg
    24601 cctgaacggg cagatcaaca tccctgaagg gcgcttccat gcctatggtc aggatctgat
    24661 tgtgcgtaag ggtgagctac tgttctctgg tccgccagat caaccgtatc ttaatatcga
    24721 agctattcgt aacccggatg ctacagaaga cgacgtcatc gccggagttc gcgtcactgg
    24781 tctggcggac gaaccgaaag cggagatctt ctctgacccg gcgatgtcgc aacaagctgc
    24841 attgtcttat ttgctacgtg gacaagggct ggagagcgat cagagcgaca gtgcggcaat
    24901 gacctcgatg ctgattggcc tgggggttgc gcaaagtggc cagattgtgg gtaaaatcgg
    24961 cgagacgttt ggcgtaagca atttagcgct cgacaccgag ggagttggcg actcctccca
    25021 ggtggtggtc agcggctatg tattgccagg cctgcaagtg aaatacggcg tgggtatatt
    25081 tgactctata gcaacactca cgttacgtta tcgcctgatg cctaagctat atctggaagc
    25141 cgtgtctggt gtagaccagg cactggattt gctctatcag ttcgagtttt agcaatgcga
    25201 atatttgtct acggcagttt acgccacaaa caaggcaaca gtcactggat gaccaatgcc
    25261 cagttactgg gcgatttcag tattgataac taccagttgt atagcctggg ccactatcca
    25321 ggcgcagttc cggggaacgg aacagtacat ggtgaagttt atcgtattga caacgccacg
    25381 ctggccgaac ttgatgcctt gcgcaccagg ggcggtgaat acgcgcgcca gttgattcag
    25441 acgccgtacg ggagtgcatg gatgtacgtt tatcaacgac ccgtcgatgg attaaagcta
    25501 attgaaagcg gcgactggtt agacagggat aagtaataat aaaagcacgc caccttcggg
    25561 tggcgttgtt ttatccacca gtctgaatat attccgaatt tgacgccatt cttatctttt
    25621 cttaaatgct gatttgttgc gcagtgttat aaacacctgg cacgctcaac aaggatgcga
    25681 ccacaatgaa aaaatctctt ctggctgtaa tgctgacagg actgtttgct ctcgtttctc
    25741 ttcccgctct gggaaatgtc aatctcgaac aattaaagca aaaagctgaa agtggagaag
    25801 ctaaagcaca gttggagctg ggatatcgct attttcaggg taatgaaacg acaaaggatc
    25861 tcaccctggc gatggactgg tttcgccgtg ccgcagagca gggatacacc ccggcagaat
    25921 atgtactggg gttacgctat atgaacggtg aaggtgtgcc gcaagattat gctcaggcag
    25981 ttatctggta caaaaaagcg gcactgaagg ggcttccgca agcgcagcag aatctgggtg
    26041 taatgtacca tgaaggtaat ggcgtgaagg ttgataaagc cgaatctgtg aaatggtttc
    26101 gcctggcagc agagcaaggt cgtgacagcg gccagcaaag tatgggagac gcatattttg
    26161 aaggcgatgg tgtgacgcgg gattacgtta tggcacgtga gtggtatagc aaagcagcgg
    26221 aacaaggtaa cgtctggtcc tgtaaccagc ttggttatat gtattccaga ggtttaggcg
    26281 ttgaaagaaa cgatgccata tcggcacaat ggtatcgaaa atcagcgaca tcaggcgacg
    26341 agttggggca gcttcattta gccgatatgt actatttcgg tatcggtgtt actcaggatt
    26401 acactcagtc acgggtatta ttttcccagt cggcagagca gggaaattct attgcgcagt
    26461 ttcgtctggg atatatattg gagcaaggtt tagcgggagc gaaagagccg ttaaaagcac
    26521 tggagtggta ccgtaaatcg gcagaacagg gaaattccga tggtcagtat tatttggcac
    26581 atctgtatga taaaggcgca gaaggtgtag caaaaaatcg agaacaagcc atctcctggt
    26641 acacgaaatc ggcagaacag ggggatgcta ccgcacaggc taatctcggc gctatttact
    26701 tcagacttgg ttcagaagaa gaacataaaa aggcggtgga atggtttcgc aaggccgccg
    26761 caaagggtga gaaagcggcg caatttaatt tgggtaatgc tttactccag ggaaaaggtg
    26821 ttaaaaaaga tgagcaacag gccgcaatct ggatgcgaaa agccgcagag caaggattaa
    26881 gtgcagcgca ggtacaatta ggtgaaatct attattatgg cttgggcgta gaacgtgatt
    26941 atgtgcaggc ctgggcgtgg ttcgataccg catcgaccaa tgatatgaat ctttttggta
    27001 cagaaaaccg caacattaca gagaaaaaac tgacagccaa acaactgcaa caggctgaat
    27061 tattatcgca acaatatata gaaaaatatg ccccggaagc ctgggcgaga atgcaaaagc
    27121 ttaaagcgca atcagcggta aagacgggta ataaataacg ggtaactgtc agatcaggtc
    27181 tgcgctaata cagctcaacc aatgccacct gcgggtggcg ttattttttt gcgagacgac
    27241 gcgtattctt ttttgtaact tcctcgcctt ttgcttttcc ttccgagccg ctttccatat
    27301 ccgttaacgc atgtaaaact ctgctggcgt taacaaatgt gcaggggtaa aacgggggca
    27361 cgctttgaac ggtgagtatg agtgaggccg gctaacttcc agcaagtggc tatgaacagc
    27421 aaaatggtgg tcgagtttag cttgttctta ccgttcaagc ctttaaactg ctgaaaataa
    27481 taatagtgga gtagagagtt tataaattgc gggcataaaa aaacccctac taaccgaagc
    27541 ccggcgttca gggttattac gccagaagaa cttatttatt cttggcgcgc tcgaaggaag
    27601 caacgatttc agctttagcg gcttctgcgt tttcccaacc ttcaacttta acccacttgc
    27661 ctttttcgag gtctttgtag tgctcgaaga agtgagcgat ttgcgctttc agcagctctg
    27721 gcagatcgtt aacgtcttta atgtgatcgt attctttgct cagcttggtg tgcggaacag
    27781 caatcagttt cgcatcttca ccggcttcgt cggtcatttt cagtacgcca accggacggc
    27841 agcggatcac agaacccggc tgcagcgggt acggagtcgg gaccagtacg tcaaccgggt
    27901 caccgtccag agacagggtg tggttgatgt aaccgtagtt gcacggatag aacatcgcgg
    27961 tggacatgaa gcggtcaacg aacagtgcgc cgctctcttt gtcgatttcg tatttgatcg
    28021 gatctgcgtt agccgggatc tcaataacaa cgtagatgtc ttccggcaga tctttacccg
    28081 cagggacgtt gagtaagctc atgtctgttt cctttaaaaa tatgtggcaa acaagtgccg
    28141 agtattatag ccaactcgcg ccgaatgtct tcgcttgttt tcgtcttcat tttccttttt
    28201 cgccagtttt aagacggtat acagagcagg aaaatccata accgtagccg catttttcat
    28261 agtgagatga aagcgattac aaacttgtga ttaacgtttt atttactttt ttgaagtgtg
    28321 atgtaacgca atccgttaca taacgaattg tctatagttt tttcgcgaac atcttttcac
    28381 caataataat taccccgacg aggacaaccc tatgtggaaa cgcttacttg tagtctctgc
    28441 agtctcggca gccatgtcgt ctatggcgtt ggccgctcca ttaaccgtag gattttcgca
    28501 ggtcggatcg gaatccggct ggcgcgccgc agaaaccaat gtggcgaaaa gtgaagccga
    28561 aaaacgcggt atcacactga aaattgccga tggtcagcaa aagcaggaaa accagattaa
    28621 agcggtacgt tccttcgtcg cacaaggggt ggatgcgatc tttatcgctc cggtggtggc
    28681 gacgggttgg gaaccggtat taaaagaggc gaaagatgcc gaaatcccgg tcttcttgct
    28741 tgaccgttcc atcgatgtga aagacaaatc tctctatatg accactgtca ccgccgacaa
    28801 catcctcgaa ggcaagttga ttggtgactg gctggtaaaa gaagtgaatg gcaaaccatg
    28861 caacgtggtg gagctgcagg gcaccgttgg ggccagcgtc gccattgacc gtaagaaagg
    28921 ctttgccgaa gccattaaga atgcgccaaa tatcaaaatt atccgctcgc agtcaggtga
    28981 cttcacccgc agcaaaggca aagaagtgat ggagagcttt atcaaagcgg aaaacaacgg
    29041 caaaaacatc tgcatggttt acgcccataa cgatgacatg gtgattggtg caattcaggc
    29101 aattaaagaa gcgggcctga aaccgggcaa agatatcctg acaggctcta tcgacggcgt
    29161 gccggacatc tacaaagcga tgattgatgg cgaagcgaac gccagcgttg aactgacgcc
    29221 gaacatggca ggccccgctt ttgatgcgct ggagaaatat aaaaaagacg gcaccatgcc
    29281 tgaaaagcta accctgacca aatccaccct ttatctgcct gataccgcaa aagaagagtt
    29341 agagaagaag aaaaatatgg ggtattgagg gttgctatgc ctgatgccga ttcgtaggcc
    29401 ggataaggcg ctcgcgccgc atccggcggt ggtgcgctga agcctgatgc gacgcttacc
    29461 gcgtcttatc atgcctactg ggagcacgct ttacaccggg ggaaaccatg acgaccgacc
    29521 aacaccagga aatcctccgc accgaaggat taagtaaatt tttccccggt gtcaaagcgt
    29581 tagacaacgt tgatttcagc ctgcgccgtg gtgaaatcat ggcgctgctc ggtgaaaacg
    29641 gggcgggaaa atcgacgctc atcaaagcat tagccggtgt ataccacgcc gatcgcggca
    29701 ctatctggct ggaaggccag gctatctcac cgaaaaacac cgcccacgca caacaactcg
    29761 gcatcggcac cgtctatcag gaagtcaacc tgctacccaa tatgtcggtc gctgataatc
    29821 tattcatagg tcgcgaaccc aaacgtttcg gccttctacg ccgtaaagag atagaaaagc
    29881 gcgccaccga actgatggca tcttacggtt tctccctcga cgtgcgcgaa ccgctcaacc
    29941 gcttttcagt cgcgatgcag caaatcgtcg ccatttgccg ggctatcgat ctctccgcca
    30001 aagtgctgat cctcgatgaa cccaccgcca gtctcgacac tcaggaagta gagttactgt
    30061 ttggcctgat gcgtcagctt cgcgatcgcg gcgtcagtct gatcttcgtc actcactttc
    30121 tcgatcaggt ctatcaggtc agcgatcgga tcaccgtctt acgtaacggc agttttgtgg
    30181 gctgtcggga aacccgcgag ctaccgcaga tcgaactggt aaaaatgatg ctggggcgtg
    30241 aactggacac ccacgcgctt cagcgtgccg ggagaacatt gctgagcgac aaacctgttg
    30301 ccgcgttcaa aaattacggc aaaaaaggaa cgatcgcgcc gtttgatctc gaagtacgcc
    30361 ccggcgagat cgtcggtctg gcaggcttgc tgggatcagg acgtaccgaa accgccgaag
    30421 tgatcttcgg tatcaaacct gctgatagcg gcacggcgtt gatcaaaggc aaactgcaaa
    30481 cgctacgctc gccacatcag gcttcggtac tgggcattgg cttctgcccg gaagacagga
    30541 aaaccgatgg cattatcgcc gccgcctcgg tgcgggaaaa tatcatcctc gctctccagg
    30601 cccagcgcgg ctggctacga cctatttccc gtaaagaaca gcaagagatt gccgaacgct
    30661 ttatccgcca gcttggcatt cgcacacctt ccaccgaaca accgattgaa tttctctccg
    30721 gcggcaatca gcaaaaagtg ttgctttcac gttggctact gacccgaccg caatttctga
    30781 ttctcgacga gccaacgcgc ggaattgatg ttggtgccca caccgagatc atccgcctga
    30841 ttgaaacgct gtgcgccgat gggctggcgc tgctggtgat ctcctccgag ctggaagagc
    30901 tggtgggcta tgccgaccgg gtgattatca tgcgcgatcg caaacaggta gcggagatcc
    30961 cgctggcaga gctttccgtt ccggcgatca tgaacgccat tgcggcgtaa ggagaatagt
    31021 gtgatgcctc aatctctccc ggacactacg ccgccgaaaa ggcgttttcg ctggccaaca
    31081 ggaatgccgc agctggcggc actgttgctg gtgctgctgg tcgatagcct ggtggccccg
    31141 catttctggc aggtggtgct tcaggatggg cgtttgttcg gtagccctat cgacattctt
    31201 aaccgtgcgg cccccgttgc gctactggcg attggcatga cgctggttat cgccaccggt
    31261 gggattgatc tctccgtagg ggcggtgatg gctatcgccg gagccacaac ggctgcgatg
    31321 acggtcgcgg gattcagcct gccaattgtt ttgttaagcg ccctgggcac tggcatcctg
    31381 gcaggattgt ggaacggcat actggtagcg atcctcaaaa ttcagccgtt tgtcgctacc
    31441 ctgatcctga tggtcgccgg gcgcggcgtg gcgcaactga tcacctccgg acagatcgtc
    31501 acatttaact caccggatct ctcctggttc ggcagtggat cgctgttgtt cctgccaacg
    31561 ccagtcatta ttgcggtgct gacgcttctc ctgttctggc tgttgacccg caaaacggca
    31621 ctggggatgt ttatcgaagc cgttggtatc aacattcggg cggcaaaaaa tgccggggta
    31681 aacacgcgga tcatcgtcat gctcacctac gtgttgagcg ggctatgtgc ggctattgcg
    31741 ggcattatcg tggcggcgga tattcgcggt gccgatgcca ataacgccgg gttatggctg
    31801 gagctggacg ccattctcgc agtggtgatt ggcggcggat cgctgatggg cgggcgcttt
    31861 aacctgctgc tttcggtggt gggggcgctg attattcagg gaatgaacac cggaattttg
    31921 ctttctggct ttccgccgga gatgaaccag gtggtgaaag cggtggtggt gctttgcgtg
    31981 ctgattgttc agtcgcaacg ctttatcagt ctgattaaag gggtacgtaa ccgtgataaa
    32041 acgtaatttg ccactgatga tcaccatcgg cgtctttgtg ttgggttata tctactgtct
    32101 gacccagttt cccggttttg cttccacaag agtgatctgc aatatcctga ccgataatgc
    32161 ctttcttggg ataatcgccg ttggcatgac ctttgtgatc ctctccggtg ggatcgatct
    32221 ctccgtcggt tcggtgatcg cctttaccgg cgtgtttctg gcaaaagtga ttggcgattt
    32281 cggcctctcg ccgctgctgg cgtttccgct ggtgttggtg atgggctgtg ccttcggcgc
    32341 atttatgggg cttctgattg atgccctgaa gatcccggcg tttatcatta cccttgcggg
    32401 aatgttcttt ttgcgtggcg tcagctatct cgtttcggaa gagtcgatcc cgataaacca
    32461 tcctgtttat gacacgctct caagccttgc gtggaaaatc cctggcggtg gtcgcttaag
    32521 tacgatggga ctactgatgc tggcggtggt ggttatcggc atattactcg cgcatcgtac
    32581 ccgttttggt aatcaggtat acgccattgg cggcaacgca acgtcggcaa acctgatggg
    32641 gatttccact cgcagcacca ctattcgcat ttatatgctc tccaccggac tggcaacgct
    32701 ggcggggatt gtcttctcga tttataccca ggccggatat gcactggcgg gcgtaggtgt
    32761 ggaactggat gctatcgcct cagtggtaat tggcggtacg cttttgagtg gtggcgttgg
    32821 aacggtatta gggacgcttt ttggcgtggc gattcaggga ctgattcaga cttacataaa
    32881 ctttgatggc acgctgagtt cctggtggac gaaaatcgcc atcggtattt tgttgtttat
    32941 ttttatagca ttacagcgtg gattaacggt gctgtgggag aatcgtcaga gttcgccagt
    33001 gacaagagtc aacattgcgc agcgataaaa acgcctctcc gtgtggagag gcgcaggagc
    33061 ttacgcgtcc gggaactcac ggataaagcg ttcgacatct tcaaccatat ggtcgttgcc
    33121 gacaaagaac gaacggcgct ggtgcagggt ttccgggata atatccagaa tacgctcttt
    33181 gccatcgctc gctttaccgc ccgcttgttc cgccaggaat gccatcgggt tgcactcata
    33241 cagtaaacgt agtttaccgt ccgggtggct ggcggtgctt gggtagagat aaataccgcc
    33301 tttcagcagg ttacggtgga aatccgcgac cagtgaaccg atataacgtg aggtatacgg
    33361 gcggttggtg gatttatctt cttcctggca gaatttaatg tacttcttca ccccgttcgg
    33421 aaacttaatg tagtttcctt cgttgatgga gtaggttttg cctttctccg ggaagcgcat
    33481 ccgttcctgg cacaggcaga aaacacccag cgaaggatca taagtaaagg cgtgaacacc
    33541 gcatccggtg gtgtaaacca gcatagtaga ggagccgtat accacgtaac ctgccgcaac
    33601 ctgtttgtta ccaggctgga ggaaatcttc ttccgttacc ggcgtgccaa caggcgtaac
    33661 gcggcggtag atagagaaaa tggtaccgac agagacgtta acatcgatgt tggacgagcc
    33721 atccagtggg tccatcagca ccacgtattt tgcgtgttca cagccttcaa agacgacaat
    33781 ctcatcttct tcttcagagg caatgcccgc aacgatatcg cgtgctttca gtgcggcttt
    33841 cagtttctca ttagcgaaca ggtcgagttt ctgctgaacc tcgccctgca cgttctcagc
    33901 accgctggca cccaggatat caaccagtcc tgctttgttg atatcgcgat ggataatctt
    33961 ggcgcccagt tttattgccg acagcaaagc agtgagctca ccggtagcat gagaaaactc
    34021 gtgctgcttt tcgacaataa attcacctaa cgttttcata aaactttccc tgcaatgttt
    34081 atggagtaaa gcgaccgcaa caatcttaac aaataatccc aatgttgcgc tcaggtgaat
    34141 cgcgccagca aattacggat tatcctgaaa tgcgtttctc acttgcccga catatgcgta
    34201 aaatgagcgc cagattaaaa aaggatagtg acgtatgcgc attcatattt taggaatttg
    34261 tggcacgttt atgggcggtc tggcgatgct ggcgcgccag ttaggccatg aagtaacggg
    34321 ttcggacgcc aatgtgtatc cgccgatgag caccttactt gagaagcaag gcattgaact
    34381 gattcagggt tacgatgcca gccagctcga tccacagccg gatctggtga ttattggtaa
    34441 cgccatgacc cgtggaaatc cgtgtgtgga agcggtactg gaaaaaaaca tcccttatat
    34501 gtcaggtccg cagtggctgc acgattttgt gctgcgcgac cgctgggtgc tggccgttgc
    34561 cggtacacac ggcaaaacca ccacggcggg aatggcgacc tggattctgg aacaatgcgg
    34621 ttacaaaccg ggatttgtga tcggcggtgt gccggggaac tttgaggttt cggcgcgtct
    34681 gggcgaaagc gacttctttg ttatcgaagc ggatgagtac gactgcgcct tcttcgacaa
    34741 acgctctaaa tttgtccatt actgcccgcg tacgctgatc ctcaacaacc ttgagttcga
    34801 tcacgccgat atctttgacg acctgaaagc gatccagaaa cagttccacc atctggtgcg
    34861 tatcgttccg gggcagggcc gtattatctg gccggaaaac gatatcaacc tgaaacagac
    34921 catggcgatg ggctgttgga gcgagcagga gctggtgggc gagcaaggtc actggcaggc
    34981 gaaaaagctg accactgacg cttctgaatg ggaagtcttg ctggatggcg aaaaagtggg
    35041 cgaagtgaaa tggtcgctgg taggcgaaca taatatgcac aatggcctga tggcgattgc
    35101 ggcggctcgc catgttggtg tagcgccggc agatgccgct aacgcgctgg gttcgtttat
    35161 taacgctcgt cgccgtctgg agttgcgtgg tgaagcgaat ggcgtcacgg tatatgacga
    35221 ttttgcccat cacccgacgg cgattctggc aacgcttgcg gcgctgcgtg gcaaagttgg
    35281 tggtacggcg cgcattattg ccgtgctgga accgcgctca aacaccatga aaatggggat
    35341 ctgcaaagac gatctggcac cttcattagg tcgtgccgat gaagtcttcc tgctgcaacc
    35401 ggcacatatt ccgtggcagg tggcagaagt ggcagaagcc tgcgttcagc ctgcacactg
    35461 gagcggcgat gtggatacgc tggcagatat ggtggtgaaa accgctcagc ctggcgacca
    35521 tattctggtg atgagcaacg gcggttttgg tgggatccat cagaaactgc tggatgggct
    35581 ggcgaagaaa gcggaagctg cgcagtaatt cacagcctca gcctgataga gtgtgtgtag
    35641 gccggataag gcgttcacgc cgcatccggc atttgagcat agtgcctgat gcgacgcttg
    35701 atgcgtctta tcaggcctac aggtgcaccg tatccggcaa accatactca catcaacaac
    35761 gaaaattacc cttcgttttc cgctaactca cgcagatact ggaaaatctg gcgcgcggat
    35821 ttcggcggct tattcccttc tttctctttc ttcgcgttgc ggatcagagt tcgcagttgc
    35881 tgacgatcgg catcgggcca cagattcaat acttcagcga tggcgtcatc accctggtcg
    35941 atcagacgat cgcgcaggtt ttccagttta tggaacagca ccacctgctg gttgtgacgg
    36001 tttttcagct tatccagcgc ctgacggatc ggttctacgt cgcgctggcg cagcatctta
    36061 ccgatgagct gcaactggcg gcggcgacct tccatcttaa tacgctgggc gagctcaata
    36121 gccgcgcgca gatccgcatc taacgggatc ttatccagcg cgtttttccc cagatcaaca
    36181 atttccgcgc caaggcgttt tagctcctcg gcatcacgtt taatttcact tttactgacc
    36241 cagataattt catcgtcttc gtcttcgatg tcatcaccgg gaacgtcgtc gagccagtct
    36301 tcgggctgct tagtcatctc aggctcctta aaaaaagagg ctaatgttac cagttaagat
    36361 gcgcactgaa aaacggttct ctgttagact tcagagaaac tctctacatt atggcacttg
    36421 caatgaaagt aatctctcaa gttgaagcgc agcgcaagat tctggaagaa gcagtttcga
    36481 ctgcgctgga gttggcctca ggcaaatcgg acggtgcgga agttgccgtc agcaagacca
    36541 ccggcattag cgtaagcacg cgttatggtg aagtggagaa tgttgaattc aatagcgatg
    36601 gcgcgctggg gatcaccgtt tatcaccaga accgtaaagg tagcgcatcg tctaccgatt
    36661 taagcccgca ggccattgcc cgcaccgtac aggcggcgct ggatattgcc cgttatacct
    36721 cgccagatcc gtatgcaggc gtggcagaca aggaactgct ggctttcgac gcgccggatc
    36781 ttgacctgtt ccaccctgcg gaagtttccc cggatgaagc cattgaactg gcggcccgcg
    36841 ctgaacaggc ggcattgcag gcggataaac gcatcaccaa taccgaaggt ggcagcttta
    36901 acagccacta cggtgtcaaa gtttttggca acagccacgg catgttgcag ggttactgct
    36961 caacgcgtca ttcgctctcc agctgtgtaa tcgccgaaga aaatggcgat atggagcgtg
    37021 attacgccta caccattggg cgtgcgatga gcgatctgca aacgccagag tgggtcgggg
    37081 ccgactgtgc tcgccgtacc ttatcgcgtc tgtcaccgcg taaactttct accatgaaag
    37141 cgccggtgat ttttgccaat gaagtggcaa cagggttgtt tggtcatttg gtgggggcga
    37201 tagcgggtgg tgcggtctac cgtaaatcga cctttttgct cgactcgctc ggtaaacaaa
    37261 ttctgccgga ctggctgact attgaagaac atccgcatct gctgaaaggg ctggcatcta
    37321 caccattcga cagcgaaggt gtgcgcaccg agcgccgcga tatcgtcaaa gatggcatcc
    37381 tgactcagtg gctgctgacc agctattcgg cgcggaaact ggggctgaaa agcaccggac
    37441 atgcgggcgg tattcacaac tggcggattg ccggacaagg gctaagcttc gaacaaatgc
    37501 tcaaagagat gggcaccggg ctggtggtga cggagctgat gggccagggc gtgagtgcca
    37561 ttaccggtga ttattcccgt ggtgcagcgg gcttctgggt agagaatggc gagattcagt
    37621 atccggtgag cgaaatcacc atcgctggta atttaaaaga tatgtggcgc aatattgtca
    37681 ccgtcggtaa cgatattgaa acacgcagta atatacagtg tggttctgtg ctgttgccgg
    37741 agatgaaaat cgccgggcag taaaaaactg gcgcgacctg ccgcgctata aactaaaaaa
    37801 ttacacaaaa tcattcgcac tgcatcgaga cggaaagtga atgagagccg gggagcgtac
    37861 tcgtagtacg tgactcgggc gaatgagcgt agccgtcgat gaggcagtgt gaaggatgaa
    37921 gtgtaaataa aaaaggaagt gagcaatgcg taaaagcctg ttagctattc ttgcagtctc
    37981 ctcattggta ttcagttcgg cgtcgtttgc cgctgatctg gaagacaata tggaaaccct
    38041 caacgacaat ttaaaagtgg tcgaaaaagc cgataacgcg gtgcaagtca aagacgcgtt
    38101 aacgaagatg cgcgctgcgg cgctggatgc gcaaaaagcg acgccgccga agcttgaagg
    38161 caaatcaccg gacagcccgg aaatgaaaga cttccgccac ggtttcgaca ttctggtcgg
    38221 tcagattgac gacgcgctga agctggcaaa tgaaggtaaa gtaaaagaag cgcaggctgc
    38281 cgcagagcaa ctgaaaacga cccgcaacgc ctatcaccag aagtatcgtt aattctttat
    38341 ttccctgttg cctgcactca ggtaacaggg aacttatcat cgcaaatgat gcaccacctg
    38401 attgctgctg ccgcgccaga tcagggaagg gtcttttaaa tcctgcacaa atttgccgtc
    38461 gaccagcacg ttaatcaaat caacaacctg catttgcgca gcgttgagtt cgtcgagttt
    38521 atagcctgtc cacacccaga tgtctttacc cggacactcg gcgcggatgc gttgtaccag
    38581 tttcagaata tccggcacgt tttgcggatg cagcggatcg ccgccggaga gggaaatacc
    38641 ctggcgtttg atgcgagtgt cattcagatc gttaatgatc tggtcttcca ttgctttggt
    38701 aaatggctga ccggaattta cccgccaggt gcttttgtta tagcaaccgg ggcattcatg
    38761 aacacacccg gagacaaaca gagtgcagcg agtgccgggg ccgttgacga tgtcgacagg
    38821 atagtactga tgatagttca ttatttcacc tggcttatca gtccgcgtgt aggctgcatc
    38881 cggtaatcaa cgcctgatgc gacgctgtct cgtcttatca ggcctacagc cgttgccaaa
    38941 tgtaggccgg ataaggcgtt tacgccgcat ccggcatttg cttaacacag agtgaagatt
    39001 aacctatctg cccatttccc aaatgtttaa cgcggcgctt aacttcttcc tgcttaccag
    39061 cgttaaacgg acgtgcatcc gggctaccta aatatccgca cacgcggcga gtcaccgaca
    39121 cacgggaggt gtcatggtta ccacatttcg gacaagtgaa gcctttgctg gtgcactcaa
    39181 attcgccggt aaagccacac tcgtagcatt catcgatcgg tgtgttggtg ccgtaatacg
    39241 gtacatgctg atagctgtaa tcccagacat cttccagcgc cttcaggttg tgctgaatgt
    39301 ttggatactc gccatagcaa atgaagccac cgttcgccag tggcgggtaa ggcgcttcaa
    39361 agtcgatctt gtcgtacggg ttcaccttct tctccacatc gaggtggaaa ctgttggtgt
    39421 agtaaccttt atcggtcacg cccggcacca cgccaaactc agcggtatcg agacggcaga
    39481 agcggtcgca caggttttca ctcggcgtgc tgtagagact gaaaccataa cccgtttctt
    39541 ctttccactg atccactgcc tgacgcagac gttcaacaat cgcgatacct ttcgcgcgca
    39601 gctgctcgtt gtcgtaaaca tgctcgccgc cgaacagcgc gttaatggtt tcgtggatgc
    39661 cgatgtaacc cagcgaaata gacgcacgac cgtttttgca gatttcagaa acatcatcgt
    39721 cagcattcag acgcacgcca caagcacctt ccatatagag gatcggggcc acgcgcgctt
    39781 tcacgccttc gagacgagcg atacgggtca tcagcgcctt acgtgccagc accagacgtt
    39841 catccagcag cttccagaag gtggcttcat cgccttttgc ttccagagca atacgcggca
    39901 ggttcaggct gatcacgccg aggttgttac gaccatcgtg gatctgctcg ccgttttcat
    39961 tttcccacac gccgaggaag ctgcggcagc ccatcggggt tttaaacgaa cctgtgactt
    40021 tcactacctg atcgtagttc aggatatccg gatacatgcg cttgcttgcg cactccagcg
    40081 ccagctgttt gatgtcgtag ttcggatcgc cttttttatg gttcaggcca tcgcgaatcg
    40141 caaacaccag tttcgggaac accgccgttt tacggttttt accaaggcct gcgatacggt
    40201 tacgcaggat agattcctga atcaggcgcg attcccagct ggtgcccaga ccaaaaccaa
    40261 aggtcacaaa cggtgtttga ccgttggcgg tgtggagggt gtttacttcg tactccagcg
    40321 actggaaggc atcgtagcac tctttgatgg tacgagagtt agcgtagcct tcggcgtccg
    40381 ggatgttcca ctcttctgcg gtcttgcgat gtttgttgta gctggcggtg acaaacggtg
    40441 ccagcacttc atcgatacgg ttaatggtgg tgccgccata aatatggctg gcgacctgag
    40501 caataatctg cgcagttacc gcggttgccg tagagatcga tttcggcggt tcaatctcgg
    40561 cgttccccat tttaaagccc tgggtcagca tgcctttcag gtcgatcaac atgcagttga
    40621 acatcgggaa gaacggtgag taatcgagat cgtgatagtg aatatcacca cgttcatgtg
    40681 cctgcaccac gtcacgcggc agcaggtgct ggcgtgcata gtgtttagcc acgatcccgg
    40741 ccagcaggtc gcgctgggtt ggaatcacct tgctgtcttt gttggcgttt tcgttgagta
    40801 acgaggagtt ggtctgctcg accagaccac ggatctcctg gttcaggcga ccgcgttttt
    40861 cacgttcaat gtcgcgatcg tgacggtact cgatgtaagc acgagccagt tgtttgtatg
    40921 gccccgacat cagctggttt tcaactgcgg tctggatctc attgatatcc acctggttgc
    40981 ggccctgcat ctgctcgctg acaaccgcag caacagtggc gcaataatcg gcatcatcga
    41041 cttccgctgc tttagctgca cgcagaatcg cttctttgat gcgctctgat ttaaacggca
    41101 ctttgcagcc gtctcgtttc atcacatgcg gtgtcatgat cgctccatat ttttaagaac
    41161 aggttatcca cagaaattgg gaaaggcgtt ttccggtttt ttcgtttcgt tttccggtgc
    41221 tttccgcaat cgccatccgc ttatccacaa cgtcgcacct ttttcgtggg gagctgtagt
    41281 agcaattata gtcgattaat acaacatatt gggttgggac gcattttaaa gtctatatat
    41341 agtgctttgc atcaaggatg tttgaacttt ttttgatgta gctcaaagta aaaagcagag
    41401 cgtacggatg acgggcgcta cagcgatatg taaatttttt aatgaatttt ttgattataa
    41461 aatcaacaaa aacaacatac tgacaggcaa aaccccggaa tgacaggcat taccggggct
    41521 tagcgaagat ttacttctgt aaccaccaga cagcctcaaa aggccgtaaa gtcatggtac
    41581 agggttgtgg tgaggcttct tcgtagttat gcatcacaag ctgccagttg ccgagcattt
    41641 tccctggctg ccagggttgg gtctcacggc taaggttggc aatgaccagc aaggtttgcc
    41701 ccttccactc acggcgatag caccacaata cagggctgtt tggcagcaga tcctggtaat
    41761 cgccccatgt caggacggct tcttgcttac gcagtgcgat taacttttgg taggtgtaaa
    41821 acaccgagga ctcatcggcc agcgctgctt ctacgttgat ttcctgatag ttatcgccca
    41881 ggccaatcca cggttcgcca gccgtaaacc cggcattatc gccgttggtc cattgcatgg
    41941 gtgtgcgact gttgtcacgg gatttactgg cgaggattgc caataactcg tcggcatcac
    42001 gcccatcgtt gcgcagctcg gcaaacatat tgaggctctc cacgtcgcga tagtcagtaa
    42061 tgcgcgtgaa atgtgggttg gtcatgccaa tctcttcgcc ctggtagata tacgacgttc
    42121 cctgcatgcc atgcagcacc atcgccagca tttttgccgc aggcacgcgg tattcacctt
    42181 catcaccaaa gcgagaaaca atgcgcggct gatcgtggtt acaccagaac aaggcattcc
    42241 atgccacgtt gtgcattcct tgttgccagt ggcggaacaa tgttttcaac gctacaaagt
    42301 caggtttagc cagcgtccat ttttcacctc cgggataatc gaccttcagg tgatgaaaat
    42361 taaaggtcat cgacaattca ctgcctgtca gtgccgcgta tcgctggcaa tgctcaaggc
    42421 tggtggagga catttcacct acggtcatta acccgcgtgg cgtaaacaca tcgcggttca
    42481 tctcgtgcaa aaactcgtgt gctcgtggcc cgtcggtata gaagcgacgc ccgtcgccat
    42541 ccaggtcttc agggtaacgc ggatctttgg agatcagatt caccacatcc aggcgcaacc
    42601 cgtcgacccc acgatcggcc cagaactcac agactttttt caactctgcg cgtaccgctg
    42661 gattctccca gttgagatcc gcctgttctg gtgcaaagag atgcaaatag tactgttcgc
    42721 tttctgcatg ccagcgccac gcactaccac cgaattttga acgccagttg ttcggtggcg
    42781 tttctggttc tccatcgcgc cagatataaa actggcggta agggctttct ttgttcagcg
    42841 cctcgcgaaa ccaggcatgt tgggtagagg tatggttaaa caccatatcg agaatgatac
    42901 gaatcccacg cgattttgcc tgtgtcacca gttcgtcaaa atcgtccagc gtgccatagg
    42961 tgggatcaat cgccgtataa ttcgctacgt cgtaaccgtt atcgacctgg ggagagacat
    43021 aaaagggggt tagccagatg gcatcaacgc ccagtttatg cagatagtcc aggcgttgga
    43081 taacgccacg taaatcgccg gtgccgctac ccgtggtgtc ctgaaaactc tttggataaa
    43141 tctggtagat aacgccgttt tgccaccagt ggggaagatg agtcattacg ttattcctgc
    43201 aaatgcgagg gggcgcaatt gcgccccgaa gaaaattaaa caatgtccag cgtgcccagg
    43261 cggtatttcc gctgatagat aaacgaggtg agtacaatcg ggatgatgat ggcgatagcc
    43321 attgccagcg caaacacctg ccagtagctc ggctgaatcg agagaattcc cggcaggccg
    43381 cctacgccga tgccattcgc cataacgccg ttcaggccgc atagcaatcc tgccagacca
    43441 gaaccaatca tcgcgcacag catcgggaag cgatatttca ggttgatgcc gtacatagca
    43501 ggctcagtga ctccaagcca ggcggagata gcggcaggca cggagatctc gcgttcattg
    43561 tgcttgcggc tggaaatgat aatacctatc acggcggagc cctgagcaat attcgacagc
    43621 gcaatcagcg gccacactgg cgtgccaccc atgctttgaa tcatctgcaa atcaatagca
    43681 agcgtggtct ggtgtacgcc ggtgatcacc agcggggcgt acaggaagcc aaacaatgcc
    43741 gcgccaatcg gagcaaagct gccggtcatc aggtgacgta ccgcaaaggc aacgccatcg
    43801 ccaatcatgc gaccaaacgg accaatcagc gcatgggcga ggaacaccgc gaggatcagc
    43861 gaacagacgg gcaccaccac cagatagagg taatccggca cgatgcgctt aagacgagtt
    43921 tcaataacgc ccagcgccag tccggctaac agtgccggga tgacctgcgc ctgatagccc
    43981 actttggcga tgctgaatat gccaaagtcc catacttccg gcagctgctg cccgagcaga
    44041 taagcgttca tcagctgtgg agaaaccagc gttacgccaa gcacgatacc gaggatcggt
    44101 gtaccaccca tttttttcac cgctgaccag caaatcccga ccggcaggta gaagaagatc
    44161 gcttcaccga tcaaccacag aaaatcgtag atcgtttgca gggaagggta catttgcgcc
    44221 agcgtctgac cgttgctcat gggcaaatcg ccgatcacat tgcgaaaacc gaggatcaaa
    44281 ccaccactaa tcaacgcggg cagcaatggg aagaagatct ccgcgaaatg agagatcaac
    44341 tgctcatgcc atttcatatt ctgccgggcg gcttttttta cctgctcttt atcaacctgc
    44401 gcctgtccgg ttgacgcaat cagtgcttga tagtaatcac ccacgttggt gccaatcacc
    44461 acctgaaatt gcccggcatt ggtgaaacag cctttcacca tggggagttg ctcaatttct
    44521 ttcggtctgg cattggccgg ttggttgagg acaaagcgca ggcgagtaat acagtggctc
    44581 accgtcgcaa tattgccgcg cccgccgacc atttcaatca accgatcgat atccgtttgg
    44641 tttattttgc tcatcataaa gccccatggc agatgacatt tttggttggc tgcagaatat
    44701 attgcgcgga aaaatttaaa aacgggaacg ttcccgaaac acagcgaaga tcacaattta
    44761 tcgttcggga aacgatcagg acagggtggc ggggatgatg atttgttgcg gttcgctgcg
    44821 cccggtgacc tgcgcgatca actggcaggc cgcctggcgt ccagcttcgg cgtaaccggg
    44881 atctacggtg acgatctccg gatggaggaa tttcattaac ggtgtattac cgacgctcgc
    44941 cagttgcaag gtgtcgatgc gttgctcttg caggtattta cttgcgccaa gtgccagcgt
    45001 gtcggttgcg cacagtaacg cggtagtttc aggcgtaatc acttttgcta cgttctcata
    45061 gccttgcttc atagcaagcc ctggcagagc ggcaacggga tgcagtttat gcgctttgca
    45121 gaacgccagg taggcttcgt gacgtcgctt accggtcgtc acgtcactgt gcggcacgcc
    45181 gagatagctg atattacgat gtccctggtc atacagccgt tgcatcagga tcttgattgc
    45241 cccttcgtcg tcataacaga ccgaagcaaa gccttttgcg tcacgcgcca gcagaaccag
    45301 cgatgactgc cagtgggcta acatttcttc tgttatgccg gtaaaaccga acagcactac
    45361 gccgtcgata ttacgccgtt tcagcacccc caaatgttcg gcaactaatt gtggggaaaa
    45421 ctgactttcc atcatgattg gatcgtaacc ttgttcatag aacgctggca gcatggtttg
    45481 aacggcgaga ttttctgaca acgaatccag acgggtaaca atgatggcga ccactttatc
    45541 gctttgccca cgcatagcgc gcgcagagcg ggaaggggaa aatccatgct gattcatcac
    45601 tgcttcaaca cgctcgcgcg tgcgctggct cacgccgctt tcgttattca gcacccggga
    45661 aaccgtagat ttccccacgc cgcttaagcg cgcgatgtct ttgatggtca gccgattttg
    45721 catcctgttg tcccataacg tgttgtttaa ttatttgagc ctaactttac ctgtgcattc
    45781 agcaatgggt aaagtctggt ttatcgttgg tttagttgcc agcaggtatt atatcgccat
    45841 aaatgctacg aatattattg gattctcctt attatttgcg gcgctttttt cacttaccgg
    45901 aggttatatg gaacctgatc ccacgcctct ccctcgacgg agattaaaac ttttccggta
    45961 agcccgtctt ttcacggcgt taccggatgc gtaaggccgt gacgttttaa cgtccctgct
    46021 cagctttatt accttcaggt aaggcttcgc cacgcctgaa gacatttctg tactgtttca
    46081 gacagtgcgg agggactcct tatgtttaaa gaaattttta cccggctcat tcgccattta
    46141 ccttcccgtc tggttcatcg tgatccattg cctggcgcgc agcagacagt gaatgcggcg
    46201 gtcccgcctt ctttaagtgc gcattgcctg aaaatggcgg tgatgcccga agaagaattg
    46261 tggaaaacgt tcgacaccca tccggaaggg ttaaatctgg cggaagtgga atctgcccgc
    46321 gaacaacatg gtgaaaataa attacccgca caacaaccgt cgccgtggtg ggtacattta
    46381 tgggtctgct atcgcaaccc ctttaatatt ttgctcacca ttcttggtgc tatttcttac
    46441 gccacggaag atttatttgc cgcaggtgtt atcgcgctaa tggtcgctat ttctacgttg
    46501 ctgaacttta ttcaggaagc tcgttccacc aaagcggcag atgccctgaa agcgatggtc
    46561 agtaataccg cgacggtact gcgagtaatt aacgacaaag gcgaaaatgg ctggctggag
    46621 atcccgatag accagctggt gcccggcgat attataaaac tggcggcggg ggatatgatc
    46681 ccggcagatt tacgtatctt gcaggcgcgg gatctgtttg tcgctcaggc gtcgttaacc
    46741 ggtgagtctc tgcccgtaga aaaagccgct accactcgcc agccggagca cagcaatccg
    46801 ctggagtgcg acaccctgtg ttttatgggc accaccgtgg tgagcggcac ggcacaagca
    46861 atggtgattg ctacaggagc caacacctgg tttggtcaac tggcggggcg tgttagtgag
    46921 caggaaagcg agccgaatgc ctttcagcaa gggattagcc gcgtcagtat gctgctgatt
    46981 cgctttatgc tggtgatggc tccggtggtg ttgcttatca acggttacac caaaggtgac
    47041 tggtgggaag cggcactgtt tgcgctttcg gtcgcggtag gcttaacgcc agaaatgttg
    47101 ccgatgattg ttacctcgac gctggcgcgt ggcgcagtca agttgtcgaa acagaaagtg
    47161 atcgtcaaac atctggatgc cattcagaac tttggcgcaa tggatattct gtgcactgat
    47221 aaaaccggca ccctgacaca ggataaaatt gtgctggaga atcataccga tatctccggt
    47281 aaaactagcg aacgcgtgct gcatagcgcg tggttgaaca gccattacca gaccgggctt
    47341 aaaaacctgc ttgatacagc ggtgctcgaa ggtacggatg aagagtcagc gcgctcgctg
    47401 gccagccgtt ggcaaaaaat tgatgagatt ccgtttgatt tcgagcgtcg tcggatgtcg
    47461 gtggttgtgg cagaaaatac cgaacaccat cagttggttt gcaaaggcgc attgcaggaa
    47521 atcctcaatg tgtgttcgca agtgcgtcac aatggcgaga ttgtaccgct cgatgacacc
    47581 atgctgcgta agattaagcg ggttactgat acgctgaatc gtcaggggct gcgcgtggtt
    47641 gcggtggcga cgaaatacct gccagcgcgt gaaggagatt accagcgggc ggatgaatcc
    47701 gacctgatcc tcgaaggata tattgctttc cttgatccgc caaaagagac aacagctccg
    47761 gcactgaagg cattaaaagc gagtggaatt accgtaaaaa tccttactgg cgacagtgag
    47821 ttagttgcag cgaaagtgtg ccatgaagtg gggctggacg cgggagaggt ggtcattggt
    47881 agtgatattg aaacgctatc tgacgacgaa ctggcaaatc tcgcacagcg caccacgctg
    47941 tttgctcgtc tgacgccgat gcataaagaa cgcatcgtaa cgttgctgaa gcgcgaaggg
    48001 catgtggttg gctttatggg cgatggtatt aatgatgcgc ccgccttacg cgctgcggat
    48061 atcggcattt ctgtggacgg cgcggtagat attgcccgtg aagcggctga tatcatccta
    48121 ctggaaaaaa gcctgatggt gctggaagag ggagttattg agggacgtcg cactttcgcc
    48181 aacatgctga aatacatcaa aatgacggcg agctctaact tcggtaatgt gttcagcgtg
    48241 ctggtggcga gtgctttctt gcccttcctg ccgatgttgc cgctacactt gcttattcaa
    48301 aatctgctgt acgatgtgtc acaggtggcg atcccatttg ataacgtcga cgacgagcaa
    48361 attcaaaagc cgcagcgttg gaatccggcg gatctggggc gctttatggt cttcttcgga
    48421 ccgatcagct cgattttcga tattctgacg ttttgcctga tgtggtgggt attccatgcc
    48481 aacacgccgg aaacgcaaac gctgttccag tcgggctggt ttgtggtggg cttactgtcg
    48541 caaacgctga ttgtgcatat gatccgcacc cgccgtgtgc cgtttattca gagctgtgca
    48601 tcgtggccgt taatgatcat gaccgtgatc gtgatgattg tcgggatcgc attgccgttt
    48661 tcaccgctgg ccagttatct gcaactgcag gcgctgccgt taagctattt cccgtggctg
    48721 gttgcgattc tggcagggta tatgacgtta acccagttgg tgaaaggttt ctatagccgt
    48781 cgttacggtt ggcagtaaag aataaactgg gcatggtggc ccggaatcag attgcagaca
    48841 aagtgagctt tgttcatgcc ggatgcggcg taaccgccta atccggtcta caaattcaat
    48901 aaattgcagg aatcacgtag gcctgataag cgtagcgcat caggcgattt tgcttttgtc
    48961 atcagactta atccgggcat gatagcccgg atttccatca agattagcga cgaacagcga
    49021 tcgcttcgat ctcaatcttc acgtctttcg gcagacgggc tacttcaacg caagaacgtg
    49081 ccgggaaggt ggcgttgtgt tcggtgaaga aggcttcgta ggtagcatta acggttgcga
    49141 agtcgttcag atctttcaca aacacggtgg ttttaacgat atcgcccact ttcaggccag
    49201 cggcttcgac gatcgctttt acgttatcca gcgactgacg tgcctgtgca gcgacatctg
    49261 ccgggacttc gcccgttttc gggtttaccg ggatctgacc ggaagtgata atcatattgc
    49321 ccagatcaac gccctgtacg taaggaccga tagctgccgg tgcattttcc gtcgcgatag
    49381 ttttgctcat gatttctcct ttattacagc ggtaaaaagt ctggctcatt atagggagcc
    49441 agacttttat taccaaccgc aattaattgg ccagcaccac attatgagaa aactcttttt
    49501 cacagtattt gcatttgagc gcgatatcat tggcgcgttt tcgcacggca aagctggatg
    49561 aaaccggttc ggcatggctg atacagttgc tgttagggca gaccagcaca ttgtcgatgc
    49621 gctctggcaa actggggcgc gatttgccaa ccacttcata gttgtcgata cggttaaccg
    49681 tggcttgcgg tgcatataac gccagctgat caacctgatc ctcacttaaa aaggtatttt
    49741 cgattttgat caaatctttg cggcccatct cgccagaagg caaattcaga ccaatggtga
    49801 tgcgctgatc tgtttcggtc aacttgaaca gactcaacag cttaaaaccg atctgggcgg
    49861 ggatatggtc aattaccgtg ccgcgtttaa tagcttcaac ctgcaattta ttatcgtgtg
    49921 tcatctctat ttccccttac agtaccagat cgcgattcag aaccagtgcc agtaacgcct
    49981 ggcgagcgaa aatcccgttg cctgcttgct ggaagtacca ggcgtgaggc gttttatcca
    50041 catccgtcgc aatctcatca acacgcggca gcggatgcag cactttcata ttggctttgg
    50101 cgttgtggag atcgctggcg cgaagaacaa actgcgcttt cacgttggcg tactcggacg
    50161 ggtccagacg ctctttttgc acgcgggtca tgtacaggat gtctacttcc gccatcactt
    50221 cttcaataga gctgtgcaga ctccaggcga tccctttttc atcgagcata tccagaatgt
    50281 attgcggcat tgccagcgca tccggcgcga taaagtaaaa acggttgccg tcgaacttcg
    50341 ctagcgcctg ggtcagggag tgaacggtgc ggccatattt caggtcacca accattgcga
    50401 cgtggagatt gtccagacgc ccctgggttt cctgaatggt gaataagtcc agcaaggttt
    50461 gcgtcggatg ttggttggag ccatcaccgg cattcagtac cggtacattg ccggaaaact
    50521 cggtggccag gcgcgctgca ccttcctgcg gatgacgcat cactatcgca tcgacgtaag
    50581 tgctgataac cgaaatggta tcggccagcg tttcgccctt tttacccagt gatgtattgg
    50641 cgctgtcgga gaagcccacc acgctggcac ccaggcggtg catggaagtt tcgaaagaga
    50701 ggcgggtgcg ggtagaggct tcgaagaaac agctggcaat aactttgtgc ttcaacagct
    50761 ctggttgcgg gttcgctttc agtttcgccg ctgtcgccag caccagatta aggtcatcgc
    50821 gactaaggtc gtttatggaa atgatatgtt tctgatatag cggattagcc atcttttatc
    50881 tcctgacgcc tgggcaaaaa aaagcccctc gattgagggg ctgggaatgg gtgatcaacg
    50941 ggaagaaaaa cggcaggcca gcgtcttttt tcagacgcgg taagacaaaa tgtcgaacac
    51001 actgaaccat acatcctccc ggcaaattgt ccggcattat actcagctct gagagaggat
    51061 caagaagatg atgcagttat aaagcaaggc aaacggttat catgaatata tattcaattt
    51121 taataagtaa ttaatttgct tgtgcaccag ggctgtacgc tttaccacac gtggtgcgac
    51181 ccaaacgata ttgctgccag caatcacgcc aagaatttct ggcaaagcat ggaaatcaag
    51241 gattcgcgct actgcccgac catatccggc gacggtatgg ataaggataa attcaccatt
    51301 atgctcgaca ctcaggacca tttcagcgac ggatcgacct gcgtcaggtg tgggaagtaa
    51361 ttgaggattc accgaataaa ttttttgtcc ttttgtattt ctaattttta tgactcccag
    51421 caatttcagt agtcgtgaaa cggttgactg actgatactg tcaaagccat ggttttgcaa
    51481 atcgcggcgg atttcctcct gtgaaagata acttttttcg gtaatcaggc gctgacagac
    51541 cgccagttgc ttcttttctt tggcggagta atcatcgtat tccttcataa tgcacctttt
    51601 atttattatt acctgatgat atttttcaca ttttataatg tgagagtggt tcatttatca
    51661 ctacaccaga tttgaaggcc gctttgataa gcggcctgtc gcgttaaatc atcgcggcga
    51721 tactcagcac aatcatattc aacatcgtga gaatcagcag tagcggtgct acccatctca
    51781 gataacggac ccagggtaca cgggcaatcg ccagaccgcc catcactacc gcagaggttg
    51841 gcgtaatcaa attaaccacc ccggaagcag actgataagc ggtcaccacc agatcgcgtt
    51901 gtacatgagc aaaatcagcg agtggagcca atattggcat cgttaacact gcaaggccgg
    51961 aagatgaggg aaccagaaaa gagagcaata cttccagcca ataggtcacg ttgatgaaaa
    52021 cagtggtgga aagccctgaa accagattct cagcactgtg cagaattgtg tgcgtaatca
    52081 tgccgttatc catcacgacc acaataccgc gggcgatgcc gataatcagt gccacaccaa
    52141 ggagatcccg cgcgccgtca ataaaggtac tggtgaaggt ttcttcgccc atgcgggcaa
    52201 tgacacccac gatgaccgca gctgccagaa agacagcaga gatttccgcc atccaccaac
    52261 ccagtaccgc tacgccgtag atcataaagg caaaggatgc agcaaaaagt atcaggatcg
    52321 ctttgcgcgt gggggtaaat tccagcatcg tttctgaacg atttcccaga aaatgcgcct
    52381 ggttttcagc catcttatcc gccacgatag agctttccgg actgttgcga actttgcggg
    52441 cataacgcat aacccagtaa acacaaatca cgtagcctac taacagcaaa ataacacgca
    52501 gcaaaatgcc ctgagtaaag gggatccctg cggcgttggc agcaatgacg gtagcaaatg
    52561 ggttaatggt tgagcccagc gtgccaatgc ccgatcccaa taacactgtg gcggcagcga
    52621 ccagaggatc aaagcgggca gccatcatga cgggcaccag caacgtgtag aaggggagtg
    52681 attcttccgc catcccataa atagtaccgc cagcagcgaa gagcgccatc aggatcggaa
    52741 tcatccactc ttcccgacca ttaaggcgag ttgtcactcg ttcgataccg gcatctattg
    52801 ccccagtttt atttactact cccaaaaatc caccgatgat caggacaaac agcgcgacat
    52861 caatcgctcc cgcagtgtag gtttgatggt tgtacaagcc gtcgatgggc gctaacagga
    52921 cagccgtaat accctgtggt tgtgcctcaa caggtgcgta agtgcctgca acaggaactt
    52981 cctttcccag tgcggcattt gtcaccatct gatattttcc cgcaggtaca atccaggtca
    53041 ttgccgcaac taacgcaatc agcacgaaca agatggtata agcggtggga aatttgaatt
    53101 tacccataat gtgatctcca taatcacggg catacgccac tcgtcgacgc atgcccgtca
    53161 gggttagtcg ccgagagttg ctaccatcac cgctttaatg gtgtgcatcc ggttttctgc
    53221 ttcatcgaaa acaatagagt gggccgattc aaaaacctct tcagtaactt ccagtccttt
    53281 gaggccgtag gccatctcga tctcgcgtcc tactgtggtg tgctcattat ggaaggcggg
    53341 caggcagtgc ataaatttaa cttcagggtt acgcgtggca gtaatcacct gttgattgat
    53401 ctgataaggt gccatcagac tcactcgttc tgcccaggct tcttttggct ctcccatcga
    53461 aacccaaaca tccgtataga gaaaatcaac gtcgtatacg ccttcttcca catcctctgt
    53521 cagggtgatt ctcgccccag tcacgctggc gatctcacgg cagtgtgtta ccagcgcttc
    53581 atctggccag aaagattttg gtgccaccag acggatatcc atccccattt ttgcagcacc
    53641 aaccatcagc gagttcccca tgttatttcg ggcatcacca agatacgcaa agctcagctc
    53701 tggcagcgtt ttgcctggcg catgttccag catggtcatt aaatccgcga ggatctgtgt
    53761 cgggtgaaat tcattggtca gtccgttcca taccggaaca ccagcgaact cacctaattc
    53821 ttcaacgata ttctgaccat aaccgcgata ctcaataccg tcatacatgc gtccgagtac
    53881 gcgagcggta tctttcattg attctttatg gccgatttgt gagccgctgg ggccgatgta
    53941 agtcacctgc gcaccttgat caaaggccgc gacttcaaag gcacagcggg tacgggtaga
    54001 ggatttttca aaaatcaacg caatgttttt accgaccagc gtttgttttt cgtttccaga
    54061 tttcttggcg gctttaagat tgatagccaa atcaatcagg tattgaatct ctgctggggt
    54121 gtaatcaagc agcttaagaa aattacggtt tttcagtgaa gtagccatgg ggatgtcctt
    54181 ataaaaatgg atcaagcgtg gttatggggg gtatcaaggc gaataagagt gcctttgtct
    54241 ccggcgagaa tttccggacc atctgcaaga gagccgatcc ctgcaatgcc ccggcactga
    54301 ctaacaaact ttgcgcaggc ggtgactttc ggccccatag acccggcatc aaactgcatt
    54361 tcgttgagta attccggcgt gacctgggcc agcgggcgtt gggttggctt gccccagtcg
    54421 agatatactg cgtccgcatc agtgaggatc aacaaggcgt cagcatggat ctgacttgcc
    54481 agtagcgcgg ctgaaagatc tttgtcgata accgcttcaa tcccatggta tccatcggct
    54541 ttttcaacca ccggaacacc accgccacca ttacagatca ccagatgatc gtgggcgatc
    54601 agtgtctgga tggcatcccg ctcgacgatg cgtttaggtt gtggagaagg tacgacccgg
    54661 cggaaagagt gaccatctgc tttgaaaacc cagtcttttt ctgcctgtag tacctgagtc
    54721 tgagcgtggt cataaattgg gccgatgtac ttagttgggt tactgaaggc gggatcgttg
    54781 gcatccactt caacctgcgt cagcagcacg ctgatttcac gttgtggtag ttgatttttc
    54841 agcgcctgtt gtagcatgta gccgatcatc ccctggcttt cggcccccag aatgtcgagt
    54901 ggatagggag cgacatgggc ataggcgcta ttttgaagtg ccagaagtcc aacttgtggg
    54961 ccattcccgt gaaccagtac aacccgccag tgttgagtca gttgggcgat agttttagcc
    55021 gcaagatcga tatttttacg ttggatttct gcttcgagtg gttcgcctcg tttaagcagg
    55081 gcattaccgc cgagagcgat aaccagtgtt ggtttgtttt ccatgatctc ttccttagat
    55141 accgtcacga tgcaaagggc aactcatgca acgtgcgccg ccacgtccgc ggcctaattc
    55201 atcaccgggg atgggtaaaa cggtgatgcc tgctttgtca tatttttcgt tagtccagat
    55261 gttgcgctcg tagcccacta ccactcccgg ccgaagcgtg agcacattgt tagcgtcatt
    55321 ccactgttcg cgttctgcct caaaggcatc accccccgtg gtgattaaac gtacctggtc
    55381 aataccgagg gctttttcga tagcatggag gagtgtgctt tcttgagtac gtttcaggcc
    55441 gccgtgaccg tcaggtgtta gcgtccagca attaacatcc gggcgcacaa cttcgggata
    55501 aaccgagaag gtgtcgatat cgatatgagt cataacggtg tcaagatgca tacaggagcg
    55561 gtgtttaggc agctcaacag cgattacccg ttcagcctga cgatgtttaa acaatgcctg
    55621 agcaagaaac tcgattcctt gtggcgtggt gcgttcagac atgccgatca acacggcccc
    55681 ccgaccaatc accagcacat caccaccttc taacgtggcg tggtcataat taatattctc
    55741 gtcgccgaaa tatttaataa attcgccgcc agcaaattgt ggatgccagc gataaatagc
    55801 gcgtagatta ttggtttcac gttggcgagc aggttttgcc attggattaa tcgatacacc
    55861 gttataaatc cagcatgagg tatcacgcgt aaataaatga tttggtaacg gcttcataat
    55921 gaaatcatta atatcgtggg tatcaaccac catattttta attgaggcgg gaatttcact
    55981 ataagtcagc ccaccactta aatgacgagc aaggtcccgg tgtgacatct ctgcaagcca
    56041 ggtgcgaacg tcagtagcaa acgtagggcc gagtcggtaa tctgatattt gtgtttccag
    56101 taaccagctt ttggcttccg ggatatccaa tgtttgtgtt agtaaatcag tgagtaacag
    56161 aacttctatc ccttgctggc gcagcgtatt ggcgaaaata tcatgctctt cacctgcccg
    56221 ctcaacggac aatacatcgt caaataacaa ctcctggcaa ttggatggtg ttagacgttt
    56281 cagactgaga tttgggcgat gcaacataac gctgcgtagt tgtccaattt cagaaccaac
    56341 ataatgcttt tccatcatta ttcctttaac actttttcaa aaataaagag gagatggctg
    56401 caaacaaatt aaatgcttgt agtcaggtct gttcagtaat tttcagatgc aatgattcag
    56461 tttatttctt ccagaaaatg tgacgacttt cacaccggcg gtgatattaa taatcgaagt
    56521 tgataatatt tgatgcggtt ctgataaatg ataaagaaac aaaatacgat aatgcattaa
    56581 tacgcaaata tacgcagagt tattcttcgt tgttaattga ttgttttatt tttcaggaaa
    56641 tgcctttaag tataatgcat tgatatttat gtatatttta atattcattg catatctatg
    56701 cgaaaggata aggtgattat ttttcacagc actattttca cgggataact atgacagcac
    56761 gggcattaaa agctaagaaa atgaactgtg atctgactga gagttttctt ttaacatatg
    56821 caataacaag atattaggac caaaatacat agctaagtta taagatttat gcagcaaaac
    56881 tgcatacaaa attactcctg cttaacatca gtacaacatc tccagcaata aacatgcgca
    56941 agggcaaaac ctgatgattt ttctatggtg agaggtggct ttttttcgta taaggaaaga
    57001 agtgaaacat tgttttaata aaggaagata ttatgattgt tggcaacatc catcacttac
    57061 aatcctggct gccggaagaa cttcgcgaag caattgagta catcaaatca catgtcagcg
    57121 atgagacagc aaaaggtaag cacgctattg acggcgaccg cctgttttat ctgatttctg
    57181 aggatacgac tgaaccaggt gaactgcatc gtgctgagta tcatgctcgt tatctcgata
    57241 tccagattgt attaaaaggg caggaaggga tgacatttag cacgcaacca gcaggcgtac
    57301 cagagactga ctggctggca gataaagata ttgcttttat cgggcagggg atcgatgaaa
    57361 aaacagtgat tcttaatgaa ggggattttg tcgttttcta tcctggcgaa gttcacaaac
    57421 cgctttgtgc ggtgggagcc cccgctcagg tacggaaagc agtagttaag ctattaaaat
    57481 cgtaagtgat cactgccggg ggcgacctgt gccgccgccc ggcaaaacgt tattttgcta
    57541 atgtagcgac cataacagct tttatggtgt gcatccggtt ttctgcctgg tcaaagacaa
    57601 tacttgccgg agattcaaag acttcatcgg tgacttccat tccaccatac agaccgaatt
    57661 ctgcggccat ttttttaccc agcgtggttt gctcatcatg aaatgcaggc agacagtgga
    57721 ggaacttaac ctgcgaatta ccggtcaggg ccatcatttt gctattcacc tgatagtcac
    57781 gcagcagggc aatacgctcc gcccatttct ctttgggctc acccatcgat acccagacat
    57841 cggtatagat aaagtctgca cctttaaccc cagaggcgat atcttccgtc aaggtgattt
    57901 tacccccgtg tttttgtgcc agagcactgc attcggcgac cagcgcagct tccggccagc
    57961 atgcctgtgg cgcgaccaga cgcaaatcca gaccggtaag cgccgcagct tcgagcatcg
    58021 aattgcccat gttgttacgc gcgtcacctg catagaccag cgtcatttcg ttgaatgctt
    58081 tgccgggcaa atgctcctgc atggtgagga gatccgccag cagctgcgtg gggtggaact
    58141 cattggtcag gccgttccat actggcacgc cagcgtattc cgccagtgtt tcgacaatct
    58201 cctgaccata gccgcgatac tgaataccgt catacatgcg accaagcacg cgggcagtgt
    58261 ctttaatcga ctctttgtga ccaatctggc tgccgcttgg gccgagataa gtaacgcgag
    58321 caccctggtc atatgcggca acttcgaaag agcatcgggt acgggtcgag tctttttcga
    58381 agatgagcgc gatgtttttg ccagtgagtc tggcttcttc tttaccgctt ttcttatcgg
    58441 ctttcagctt cgcggctaac tgcaacaagc tgttgagttc agctggcgtg aaatcgagta
    58501 atttcaggaa atgcttatga taaaacccgg acatagatcc ctcctgtggc taacgcctta
    58561 atgaattaaa attcaatcta tatggatgat tattcatttg caagtctaaa gcataaatct
    58621 ttgtcacaaa ggtggaggca atgtcagtgg tgtgtgacaa taagagtatc ggcaggacat
    58681 taagaggaat gagccatggc aaacccggaa caactggaag aacagcgtga agaaacacgt
    58741 ttgattattg aagaattact ggaagatggc agcgatccgg acgcgctgta caccatcgaa
    58801 caccatcttt ccgcagacga tctggaaacc ctggaaaaag cagcagttga agcgtttaaa
    58861 ctcggttacg aagtgaccga tccagaagag ctggaagttg aagatggtga catcgtgatt
    58921 tgctgcgaca tcctcagcga gtgtgcgttg aatgccgacc tgatcgatgc ccaggttgaa
    58981 caactgatga cgctggcgga gaaatttgac gttgagtacg atggttgggg cacttacttt
    59041 gaagatccta acggcgaaga cggcgacgat gaagattttg tcgatgaaga cgatgacggt
    59101 attcgtcact aattaagcgt tgacgacaaa ggctgcgtta cctgatgcgc tacgcatatc
    59161 aggcccaggt agttcctgca acctggtgaa tttgtagccc ggataaggca tttacgtcac
    59221 atcctgcata ctcaaaaagc acgtggttaa tgttcgaatg cccgcgagtc taagctcgcg
    59281 ggcatttttc aaagtttgcg tagcatccgt acttcacaat cgacatgacc cgtgcagcca
    59341 agcgcatagt cgatatgctc aaagcccaaa tgctcataaa gcgcaatggc ttcctttaaa
    59401 aaagcggtcg tttccagata gcagcgtttg aaacccattt ctcgcgcctg ctccattgcc
    59461 attaaggcca gtttttttgc cagccctttg ccgcggatag ccggaagaaa atacatcttt
    59521 tgcagttcgc aaatgtctga ctcactcccg gttaatggcg caatcccacc gccgccgacc
    59581 acttcaccct cgtactcaac cacccaatat gcgtggccag gctgactata tacttgatac
    59641 agctcgtcaa ggttcggatc ggcgacggtg tagcctttat cagcggtaag accgtattcg
    59701 gcggatacct gacgaatgac gtgggcgata gcaggattat cggcgacggt aagtttgcgc
    59761 aaacggagtg cgggagaagc gacaacattc atagcatagc tcaatttcaa atgtatgatt
    59821 tatagctcac tttaatacca ccctcgctta ggtggtgcaa gaagaagata aatcagaaaa
    59881 tattacgcat atctctctga gataaagcgt attctaggcg aaccagtatc aattgactgg
    59941 aattattatc ggtatgaatg gaatttattt tagggatata gttcatgaat gatgtaaaca
    60001 tcgggaaaga taattctcgg cactcgtttg ttttcacagg caaaggtggt gagtattttc
    60061 ttatttgtct tgtgaatttt ttactgacaa ttattactct gggaatatat gggccgtggg
    60121 cattgataaa atgccgtcgt tatatttatc aacatgtgac actcaagggg caaccattta
    60181 gttataaggg aacaggtggt gccatttttg ttagcatgtt acttattgtg gtggtttatc
    60241 ttctcagtat ttcttgtttt gcaggtcaac actttgcgct cggtttattc ctgtttgctc
    60301 tcttgatctg cggtattcct tgtatggcag tgaagagtct gcagtatcag gcgaacatga
    60361 cctcactgaa tgacattcgc tttggcttta actgctcaat gatgcgtgca tggtgggtta
    60421 tgctaggact gcctgttctg ttggcgttag ttttttggtt cgcactgtat cttattgcac
    60481 aagtgacgac atcaataggg ggggtattct ttaacttagt cgcattaagt ttgctttctg
    60541 ccatcgggct aggtgttgtc catggtataa cctatagtaa atggatgcca ttgctgggta
    60601 ataatgcaac ttttggcatt cataagtttt ctattcaggt aaacgtaaaa gaatgcatta
    60661 aaggttgcat gctcgcaata ttaaccatgg ttcctttcat cattgtgatt ggtattatga
    60721 tcgcaccagt attccagcag ttaatgatga tgaccatgtt gggtaggtct gatgctggca
    60781 gtgagtttgt attacaatat tacccgcaga ttatggcttc ttatttcctg tattttgtcg
    60841 cgatccttgt ttttgccagt tatctctatg tcacgttacg caatctgttt ttgaataatc
    60901 tgacgctggc aaatgggaca attcgttttc attcatctgt taccgcgatc ggtatgcttc
    60961 tgcgcatgtt ggcggtatta atggggtcct caattacctg tggtctggct tatccctggc
    61021 tgaaaatgtg gatggttagc tggattgcta acaataccca tgtccagggc gaccttgatt
    61081 cgctggagtt aaccaatgat gacaaaccgc aggatagcgg ttcgttaatg tggatatctc
    61141 gcggtattat gccgtatgtt ccgtttatct aaataaaaag gccggagcat gctccggcct
    61201 tcgttttcac tatgagtatt acagcgcggc gataacagcc tgctgttcaa tcagcttcgc
    61261 tttcgcttct gcatagcctt ccagcttctc acgctctttc gcgatgaccg cttccggtgc
    61321 gcgggcgaca aagccttcgt tcgccagctt gttctcaata cggctgattt cgccttcgat
    61381 cttcgctact tctttcgcca gacgtgccag ctcatcttct ttgttgatga ggcctgccat
    61441 cgggatcagc agttctgcgc cgtcgacgat ctttgtaacg gaaaccggac ctttatcatc
    61501 agcaggcagc acggtgatgc tttccagacg cgccagcgtt tgcaggaagc cgcggttttc
    61561 atttacgcga cgttctgcat ccgcgctgca accacgcagc agcagctcca gcggtttacc
    61621 cggtgcgatg ttcatttctg cacggatgtt acgtacagca acgatcgcct gtttcagcca
    61681 ctcggtgtcg gccagtgcgg cttcatcaac ctgagacgca tcgtactgcg ggaacggctg
    61741 cagcatgatg gtatcggcag tgataccgca aagtactttc actcgctgcc agatggtttc
    61801 ggtgatgaac ggaatgatcg gatgcgcgag gcgcagcaga ccttccagta cagtcaccag
    61861 cgtatggcga gtaccgcgca gttcggcttc tgttccaccg ttcattaccg gcttggtcag
    61921 ctccagatac cagtcgcaga actggttcca ggtgaactca tacagaatgc ctgcggcgat
    61981 atcgaagcgg aagctgtcca gcgcttcgcg gtacgctttg atggtctggt tgaactctgc
    62041 cagaatccag cgatccgcca gcgacagcgt catttcaccg ccgttaaagc cgcaatcctg
    62101 accttccgta ttcatcagca caaagcggct ggcgttccac agcttgttac agaagttacg
    62161 gtaaccttcc agacgcttca tatcccagtt gatgtcacga ccggtagaag ccagcgccgc
    62221 cagggtgaag cgcagcgcgt cagtaccgtg cggctcaatg ccgttcggga actgcttctc
    62281 ggtgcgctta cggattttgt ccgccagctg cggctgcatc atattgccgg tacgtttttc
    62341 cagcagttcc ggcagcgaaa taccgtcaac catatccagt gggtcaatca cgttaccttt
    62401 ggatttggac atcttctggc cttcgtcatc acgaatcagg ccggtcatgt aaacggtgtg
    62461 gaacggcacc tgcggtttgc cattttcatc tttgatgaag tgcatggtca tcatgatcat
    62521 gcgggcaatc cagaagaaga tgatgtcaaa accagatacc atcacgctgg ttgggtggaa
    62581 ctggcgcagt gcgtcggtgt tttccggcca gccgagcgtg gagaaggtcc acagcgcgga
    62641 ggagaaccag gtgtcgagaa cgtcttcatc ctgacgcagc gcaacatcag caccgaggtt
    62701 attttcttta cgcacttcgt cttcgttgcg gccaacataa acattacccg cttcgtcata
    62761 ccatgccggg atacggtgac cccaccatag ctgacgagag atacaccagt cctgaatatc
    62821 gcgcatccag gagaaataca tgttttcgta ctgcttcggt acgaactgaa tgtcgccatt
    62881 ctcaaccgct tcaactgccg gtttcgccag tacatcggca cgcacgtacc actggtcggt
    62941 cagcatcggt tcgataacca cgccgccacg gtcgccgtaa ggaacggtca ggtcgtgcgg
    63001 tttaatttct tccagcaggc cgagcgcgtc aaccgcagca acgacggctt tacgtgcagc
    63061 aaaacgctcc agtttctgga actctgccgg gatttcgctg gaataaacgt cagattcgtt
    63121 acctttggta tcgaacacct gggcgctttc acggatatcg ccgtcaaagg tcaggatgtt
    63181 gatcatcggc agggcgtgac gtttaccgac ttcatagtcg ttaaagtcgt gcgctggggt
    63241 gatcttcacg cagccagtgc ctttttccat gtcggcgtgt tcgtcgccaa cgatcggaat
    63301 acgacggtta accagtggca gaatgacata tttgccaatc agatctttat aacgcggatc
    63361 ttccgggtta acggccacgc cagtatcgcc cagcagggtt tccggacggg tagtcgcaac
    63421 caccagataa tctttaccgt ctgcggtttt cgcgccgtca gccagcggat agcggatgtg
    63481 ccacatcgaa cctttcgatt cgcggttttc cacttccagg tcggagatag cggtgcgcag
    63541 tttcgggtcc cagtttacca ggcgtttgcc acggtaaatc aggtcttctt tatacagacg
    63601 aacgaaaact tctttcaccg cattggacag gccttcgtcc atggtgaagc gttcacgctc
    63661 ccagtcgacg gagttgccga gacggcgcat ctgacgggta atggtgccgc ctgattccgc
    63721 tttccattcc cagattttgt cgatgaaagc ttcgcggccg tagtcgtgac gggttttacc
    63781 ttcttctgcg gcaatcttgc gctcaacgac catctgggta gcgatcccgg cgtggtcagt
    63841 accgacctgc cacagggtgt ttttgccctg catgcgctga tagcggatca tggtatccat
    63901 gatggtttgc tggaaggcgt gacccatatg caaactgccg gtgacgttcg gcggcgggat
    63961 catgatgcag aaactttcct ggctttcatc gccattaggc ttaaagtagc cctgcttttc
    64021 ccagtgctcg taaagcggct gttcgatatc ttgtgggtta tatgtctttt ccattatttc
    64081 caggttgccg tattcaggtt gaaaccagcc acgcggtagg ctttatagcg ttcgcgcgcc
    64141 agttgtttca gagaatcttc ataaggaacg aagtctacca cttctgtgaa agcggtggca
    64201 aaatctgcaa agcttgttcg cagactaatc aatatatcgc gtgggttgct gctgcgcttt
    64261 tgcggccagg cgatctccac cggcgcaccg ccgcgcggtc cttctcccgc taaattatgc
    64321 ggaacaaagc tttctgccgg acgcgcccac agggcttcat ccagtcggta agcctgcttt
    64381 tcatcttcac aggcgatgag tacgcgctta ccactgcgcc aacgttctgc ggcaatttca
    64441 cacaccaact gctcaacggc gcttaagcca tcgacggtgg tgtcattgtc cagaaggtag
    64501 aacgttgcgt ttttcatata tggggcttct tgtggtggat ttaaatacgc atcaggtata
    64561 caccgcagcg cctgatgcga cgctgccgcg tcttatcagg cctacaagtt aagtgctgtg
    64621 tgtaggtcgg ataaggcgtt cacgtcgcat ccgacaataa cagccttgcc tgatgcgacg
    64681 ctgtcgcgtc ttatcaggcc tacgagttca gtgctgtgta ggtcggataa ggcgttcacg
    64741 ccgcatccga caataacaac acagcctgat gcaattactc ttcgccgtta aacccggcgc
    64801 ggtttagcag gaactgtgcc agcaacgcca ccgggcgacc ggttgcgcct ttcgccttac
    64861 cagaacgcca ggcagtaccg gcgatatcca ggtgcgccca gttatactta cgggtaaagc
    64921 gcgacaggaa gcaacctgcg gtaatcgccc caccaggacg accgccaatg ttcgccatat
    64981 cggcaaaatt ggactccagc tgttcctggt actcgtcgcc cagaggtaag cgccatgcac
    65041 ggtcaccgga ttgttcagac gcggcaatca gttcatgggc cagtgggtta tggttcgcca
    65101 tcagaccggt aatgtgatgg cctagcgcga tcacgcaggc accggtcagc gtcgccacgt
    65161 caatcaccgc ttccggctca aaacgctcaa cgtaagttaa cacatcgcac agtaccaggc
    65221 ggccttcggc gtcggtattc agcacttcta cggtttgacc ggacatggtg gttaacacat
    65281 cgcccggacg ataggcacga ccgccaggca tgttttcgca gcctgccagc acgccgataa
    65341 cgttaatcgg cagttgtagc tccgcgacca tacgcatcac gccgtagacc gctgccgcac
    65401 cgcacatatc gtacttcatc tcatccatgc cttctgaagg cttgatcgag ataccgccgg
    65461 agtcgaaggt taaaccttta cccaccagca cgattgggcg tgcatcttcc gacgcgttgc
    65521 ctttgtactc aatcactgac atcagcgatt cgttttgcga accctgaccg accgccagat
    65581 aggaatgcat ccccagctct ttcatctgct gttcgccgat aactcgggtg atgacattct
    65641 tgctgtagct gtcagccaac tggcgcgcct gagaggcgag gtaagcagcg ttacagatat
    65701 tcggtggcat attgccgaga tctttcgcgg ctttaatccc tgcggcaatc gccagaccat
    65761 gctggatcgc gcgttcaccg ctggtcagct cacggcgggt cggcacgttg aacaccattt
    65821 tacgcagcgg acgacgcggt tcgctcttgt tcgttttcaa ctgatcgaaa ctgtagagcg
    65881 tctcttttgc cgtctcgaca gcctgacgca ctttccagta gttgttacgg cctttaacgt
    65941 gcagttcagt cagaaagcag accgcttcca ttgagccagt atcattcagc gtattaatgg
    66001 ttttctgaat aacctgcttg tactgacgct catccagctc acgttctttg ccgcaaccaa
    66061 taaggagaat tcgctcggaa agtacattcg gcacatggtg cagcaacaat gtctgccccg
    66121 gttttccttc cagttcgccc cgacgtagca gggcgctgat gtacccatcg ctgattttat
    66181 cgagctgttc tgcaatcgga gaaaggcgac gtggttcgaa gacgcccacg acgatgcagg
    66241 cactccgctg tttctccggg ctaccgcttt ttacactaaa ctccatgcac tacgctcctg
    66301 aatcttaaag acaacggcgg tggctacaga tagaattgca agctttcgta actcatgtcc
    66361 gctgttgcga tgacttcgtg ttaatcttaa cgttattacg gcattggcac gtcagaacaa
    66421 aatctgagag gtgaatccgt tgagtataat tatcttagcg acgatttcga cgactcaaga
    66481 gaataaatga cgtttaagcc atgaaacaag ctaaaatcct gcaaaagacg agtttttacg
    66541 ggcgtattta aagtgataat cataagatat ctggtgcggg agacgctcaa aagccagctg
    66601 gcgatactct tcatcttgct tttgatcttc ttctgtcaaa agttagtgag gatcctcggc
    66661 gcagcggttg acggcgatat tccggcgaac ctggtgctct cccttctcgg gttgggcgtg
    66721 ccggaaatgg cgcagcttat cctgccatta agcctgttcc tcgggctgct gatgacgctg
    66781 ggcaaactgt ataccgaaag tgaaattacg gtaatgcatg cctgcggtct gagcaaagcg
    66841 gtgctggtga aagcggcaat gatccttgcg gtgttcacgg caatcgtcgc ggcggttaac
    66901 gtgatgtggg cgggaccatg gtcatcgcgt catcaggatg aagtgttagc agaagcgaaa
    66961 gcgaaccctg gcatggcggc gctggcgcaa gggcaattcc agcaagcgac taatggcagc
    67021 tcggtgctgt tcatcgaaag cgttgacggc agcgatttca aagatgtgtt cctcgcgcaa
    67081 attcgaccaa aaggtaatgc acgaccttct gtggtggtgg ccgattccgg acatttaacc
    67141 cagctgcgcg acggctccca ggtcgtcact ctcaaccagg gaacgcgctt cgaaggcact
    67201 gcactgttac gtgatttccg cattacggat ttccaggatt atcaggcgat cattggtcac
    67261 caggcggtgg cgctcgaccc gaacgatacc gaccagatgg acatgcgcac attgtggaac
    67321 accgacaccg atcgtgctcg cgcagaactg aactggcgta tcacgttggt gttcaccgtg
    67381 tttatgatgg cacttatggt cgtaccgctg agcgtggtta acccacgtca gggacgcgta
    67441 ctgtcgatgc tgccagccat gctgctgtat ctacttttct tcctgatcca gacctccctg
    67501 aaatcgaacg gcggtaaagg taagctggac ccgacgctgt ggatgtggac cgttaacctg
    67561 atttatctgg ctttggcgat tgttctcaac ctttgggaca ccgtgccggt ccgccgcctg
    67621 cgcgccagtt tttcgcgtaa aggagcggtg tgatgcaacc ttttggcgta cttgaccgct
    67681 atatcggtaa aactattttc accaccatca tgatgacgct gttcatgctg gtgtcgctgt
    67741 ccggcattat caagtttgtc gatcagctga aaaaagccgg gcaggggagt tacgacgcgt
    67801 taggcgcagg aatgtatacc ttgctgagcg tgccgaaaga tgtgcaaatc ttcttcccga
    67861 tggcggctct gcttggggcg ttgcttggtc ttgggatgct ggcgcagcgc agcgaactgg
    67921 tagtcatgca ggcttctggt tttacccgta tgcaggtggc gctgtcggtg atgaaaaccg
    67981 ccattccgct ggtcttgctg acgatggcga ttggtgaatg ggtcgcgccg cagggcgagc
    68041 agatggcgcg taactaccgt gcgcaggcga tgtacggcgg ctcgttgctc tctacccagc
    68101 aaggtttatg ggcgaaagat ggcaacaact tcgtctacat tgagcgggtt aaaggtgacg
    68161 aagagttagg tggcatcagt atttatgcct ttaacgagaa tcgtcgtctg caatccgtac
    68221 gctatgccgc taccgcgaag tttgacccgg aacataaagt ctggcgtctg tcgcaggttg
    68281 atgaatctga cctgaccaat ccaaaacaga taaccggctc gcagacggtg agcggcacct
    68341 ggaaaaccaa cctcacgcca gacaaactgg gcgtggtggc gctggacccg gatgcactct
    68401 ctatcagcgg tttgcacaac tatgtgaagt atctgaagtc gagcggtcag gatgccggac
    68461 gttatcagct caacatgtgg agcaaaatct tccagccgct atccgtggcg gtgatgatgc
    68521 tgatggcgct gtcgttcatc tttggcccac tgcgtagcgt accgatgggc gtgcgtgtgg
    68581 tcactggtat tagcttcggt tttgtcttct acgtactgga ccagatcttc ggcccgctga
    68641 cgttggttta cggcatcccg ccgatcatcg gcgcactgtt gccaagtgcc agcttcttct
    68701 taatcagcct gtggctgtta atgagaaaat cgtaacctga tgaacgaata aagggagcga
    68761 aagctccctt tattattgtt agcaaagcgt gcttcgttca tgcctgaaaa atcatgaaaa
    68821 ttcaatatat tgcagtggtg tgggcatgat aaggcgcgcc agcgtcgcat cagacaaaga
    68881 caaatcgcac ctggcttacc ttcttctccc ccccagcaaa ctccccaaca tcccgcgcac
    68941 aatctgattc gtcacctggc gggcggcgct ttttgccatt gtttgcacca caccatcttt
    69001 cttcccgccg cgtggtccag tagtgccaaa caaaatatcc ttcaatccac cgagaatacc
    69061 gtcatccacc gccacctctt tccctttcgc gggaggatta ttttgctgct cgatactggc
    69121 ctgaaagcct ttttgcaaca tctcataggc ggattcccgg tccacgtcat cctcgtactt
    69181 gccatacacc ggggagtggt taatcaagcc attgcgctca tcttccgtca ccggccccat
    69241 tcgcgaacaa ggtgcgatca ccatcgcccg ttccaccaca gaaggacttc cttttgcatc
    69301 gagaaacgag attaacgcct cgccggtccc cagttcctgg attgcctttt cggtatcaaa
    69361 tgccggattg gcccgcatgg tttgcgccgc agcttttact gctttctgat ctttgggcgt
    69421 aaaagcccgc aaagcgtgtt gaacacgatt accgagctgc ccgagcacat tatccgggat
    69481 atctgacggg ttttgcgaaa cgaaccagac gcccacgcct tttgagcgaa taagccttat
    69541 cacctgctca atcttatcca gcagtacctg tggcgcatcg ttaaacagca gatgtgcttc
    69601 gtcgaagaaa aacaccagtt ttggtttctc cagatcgccc gcttcaggca attgttcata
    69661 taactctgaa agcatccaca gcaggctggc ggcgtacagt ttcggcatct gataaagttt
    69721 ctcggcgctg aggatattga taacgccttt accgttggca tcggtgcgca tccagtcttt
    69781 gatatccagc atcggctcac caaagaagtg tgctgcgcct tgctgttcca gcgacagtaa
    69841 tccgcgctgg atggcaccaa ccgatgcgct actgatattt ccgtactgat tctggaagga
    69901 tttggcgtta tcgccgatgt actgggtaat cgcccgcaaa tctttaaagt cgagcagcaa
    69961 caatccctga tcgtccgcaa tgcggaagat aatattcagc acgccagatt gcacatcgtt
    70021 gagattcaac agtcgcgcca gcaacagcgg ccccagatcc gaaaccgtcg cccgcaccgg
    70081 gtggcctttc tcgccaaaga tatcccacac caccaccgga ttagcatgag gttgccagtc
    70141 atttacgccg atatttttaa gccttgcgag cagtttttcc gaagaagtac cttcctccgc
    70201 tactcccgtt aaatcacctt tcacatcggc cataaacacc ggcacgccga tttcagatag
    70261 agactctgcc agtttttgca gcgtaacggt tttacccgtc cccgttgcgc cagtaatcag
    70321 cccgtgacgg ttagccattc ccggcagtaa aaacagttct gtatccggcg tgcgggcaat
    70381 taacaggggt tcgctcatga gatttcctcc agtttcctgc ctggagtata ggcaacgtcc
    70441 ggcaaagaga atatgtgtaa tgcagagaaa ataacctaaa gcgtgttacc gtgataaatc
    70501 tgatacccga ggtcaacagt gttgtggttg tgatcgttat tcttaatttt gctcagcaac
    70561 atttgggcgg ccatccgacc aatatcaaaa cggggagtaa ttacgctggc gaggcttggg
    70621 atcatttgcc tgccaatctc cagcccatga aaacccgcaa tggaaatttg ttctggcacg
    70681 gcgaggtttc gctcgcggca cagcagaagc gcacccatcg caatatcgtc attggtgcaa
    70741 aatacaccgt ctaaatccgg gtttgcgcta agggtatcac gcattaactg cattcctaaa
    70801 tgaatggatg agatggcgcg tggattcatg cgtaatgggg aaagattatg cagcatcatc
    70861 gcatcgcaat acccctgata acgctgttca tcgcgggtgt catctttgga accgagatac
    70921 agaattttgc gcctgactcg cttatccagc atggtacaca ccatgtcaaa agcagcctgc
    70981 cgattatcaa aaccgacctc catatccagc cgttctccct gtacatccat caattccacg
    71041 accggaatgg tggctgaacg cagaaatttc accgtcctga tggtgtgata tttttccgaa
    71101 agaataatcc cgtcaatgtt ataagagagc aggttaatca ccgactcttc ttcggaatcg
    71161 cgatcgtaat tgtaattagc aataagggtc tgataattat gcacagaggt gacggattct
    71221 attcccgcga gaatgtcagc gaagagttga ttttgaaatg acggaatcaa gataccgagg
    71281 gtataacttt gtgcattcaa caacatgccg ggagcgcgat tagggatata attgatctct
    71341 tccataattt tggcgatgcg ctcgcctgtt tcctttgcca cctttttcgg cgagcggata
    71401 taacgactca cggtcatttt tgttacgcca gccagcgtag cgatatcctg taaagaaatt
    71461 ctgtgattgc gcataacttc tcccaacgtc gggggaatct tcaatcttcc cccgaaacta
    71521 catcacaata ttttattctt tcagtgcaat acggcgttaa tggctaacac gcccagcaaa
    71581 cccataatag aaataagggt ttccatcacc gtccaggtac gcaacgtttc accaaccgtc
    71641 agattaaaat accctttaaa tagccagaag ccagggtcgt ttacgtgtga cgcgatcacg
    71701 ctgcccgcac cggttgccag taccattaaa gcgggatcgg catgggtaac gttgataatc
    71761 ggcaacacca cacccgcggt ggtaatggcc gcgacggtgg cagagcccag agcgatacgc
    71821 aacagcgccg caacagtcca gcacatcaat aacggcgaaa gtgtagttcc ggtcattaag
    71881 tgtgaaatat actgcccgac accgctatct actaatacct gcttaaacgc gccgccgcca
    71941 gcgataataa acacaatcat cgcgatagcg cctatagaat ccccaatgat atccatgatt
    72001 tgctcgatgg tgcgtccatt acgtcggccc agtgtgaaaa tcgcaataac aatggcaata
    72061 aacagcgcaa cggcagggtt accgacaaat tcaaagaaga ggcgcacggt gttagttttc
    72121 ggtaacgtaa tttcacaaac ggcggcgata gccatcagga tgaccgggat cacggcagcg
    72181 aaaatactgt tccagaagga gggcatctcc tcttcgctaa acagatgagg attaaataag
    72241 ccttccggtg gtgctttctc aaagcgagtt agcagtttag aaaacagcgg tcctgcgaca
    72301 ataactgtcg gaatggtaat gataaatcca tacagtaaag tcgttccgag attagcctca
    72361 aagatagtcg cgatggcagt aggccctgga tgtggcggca gaaaacagtg ggttacagag
    72421 agcgctgcta ccattggtac gccaacatac aacaggggta atcctgatga tgctacgatg
    72481 gtaaatacca acggcaacag caggacaaaa cccacttcaa aaaacatggc gaggcccaca
    72541 accagaccgg tgatcactag cgcccattgc acgcgttttt taccaaaagt agcaatcagc
    72601 gtagtggcga tacgttgtgc cgcacccgta tcagaaatca gcttgcctaa catggccccg
    72661 aaaccgagga tcattgccag cccgccgagc gtgctgccga taccattttg tatagagtgc
    72721 aggacggcct gtgcatccat cccttcggca aatccgacga cggcagcgac cagaacgagg
    72781 gcaataaagc cgttaacttt aaagccgatc atcaggataa gaagcagcgc gacgcctgcc
    72841 gcaataatga ttaatggcat agtctttatc tcttattagt gccagaaaag cagcgctttc
    72901 gcgctgcacg tagggtagta ggtgttaaac agccactaac atgccgccat caacaaacaa
    72961 caggtggccg tttacgaaat cagaggcttt tgaagaaagg aacaccgcag caccaatcag
    73021 ttcctgcgga tctccccagc gtgcggcggg ggtccgtttg cacaaccagg cggtgaaggc
    73081 ttcgtcctca accagtgctt tagtcatttc tgttttgaaa tagcccggcg caataccgtt
    73141 gacctgaata ttgtggcgcg ccagctcgac gcacatgccg cgggtgagca tttttaccgc
    73201 ccctttcgat gcggcataag gggtgatggt gtcacgtccc agttcgcttt gcatcgagca
    73261 aatattaata actttacctg ccttgcgttc aaccatgtga cgagttaccg cttgcgatac
    73321 caggaacacg gcggtctggt ttactgcgat cacatcattc cactcttgtt cagggaactc
    73381 agtaaaagga tgacggcgct ggataccggc gttattcacc agcacatcaa tggggccgat
    73441 gtccttttcg atatgttcaa cggcggcatc aatttcatgt ttatgagtaa cattaaaagg
    73501 tgcggcaacg gcctgaatac cctcctggtg gagtttttct acagcaagtt ctgcgcgttc
    73561 ggcagtaata tcattaataa ttatttgtgc gccatattta cccaggccgg ttgccagtaa
    73621 aaagccaatg ccctgtgctg aaccggtaat caagatattt tttcctgcca gtgaaaatag
    73681 atcgttcatt gtattattcc tttatttatc ttttagaaaa caagctggac ttttgctgcc
    73741 tgggttttat caccggcgaa acgtagcgcc tcttccaggt ctgtgaaggg atattcagca
    73801 ctcagtaaag gcagtggatt aataacgcca ttcgccagcc atgacactgc ggtattaaat
    73861 tcgctggtaa aacggaaaga gcctttgagt gaaatctcct taccaatcaa cgtcatcatt
    73921 gggaattctg ccatcgcgcc tcccatccct acctgcacca ttacgccgcg tgcacgagtg
    73981 acctccagac aggtattcac tgatgaagga tgaccggaca cttcaaagct gacatcgaaa
    74041 tagccttttt ccgctttcca gtgatccatg tcgtcgtttt gtgggtttac gagcacatcc
    74101 gcccccatct ctttgcccag cgaaagggaa cggggactca catcggcaca gacaatttcc
    74161 gcggccccca gtgttttcac agcactgaca atcaggcagc caatgggccc aacaccagaa
    74221 ataaatactc gcttgccctg taactcgccg gcctgatgtg cggcatgaat cgcgacggct
    74281 aaaggttcgg caaaagccat aaccttttcg tcagctttgg ccggataagg gacacattgc
    74341 gacgtttcga ccattttata acgggtaaag ccaccatcaa catgagggaa atacatggca
    74401 ctgccaaaaa aacgcatctc tgtacactga ttctcgttat gttcaatgca gtatttgcag
    74461 tgaccgcacg gtttagacgg attaatggct accgtttgcc cttcatgtaa ttttgatgag
    74521 tcgctatgaa taactttacc gataacttca tgacctaaca ccatcggtgc ctttatcatg
    74581 aaattaccta cttttccttc ctgataataa tgtaaatcgg aaccgcaaat tccacctcgg
    74641 gttatttgta ctaatgttcc attattattc caatctatcg tctgctcggt aacggcaaca
    74701 gttttcttgc ccgcaacaac gcaggactgt gttttcactt gcataaattc ctcactggtc
    74761 aggtagacac ctcggaagca tttaagcggt tttaattgtc attatttgtg atgaagatca
    74821 cgttagaaaa ttgttacatt acgatgttac gcataacgtg atgtgccttg taattcttat
    74881 cagcagaaat aaaaaaacgt gaaattatta tgccacctgg cgtagtatcg cagcaggtaa
    74941 gatgattcag gagattttaa atggcgggtg aaagctttat tttgatgggc gtttcaggga
    75001 gtggtaaaac attaattggt agcaaggttg ccgcgttatt atctgctaaa tttattgatg
    75061 gtgacgatct tcatccagcc aaaaatatag ataaaatgtc gcagggtatt ccattatctg
    75121 atgaagatcg acttccctgg ctggaacgct taaatgatgc ttcatacagt ctttataaaa
    75181 agaatgaaac aggatttatt gtctgttcat cattaaaaaa acagtatcgt gatattttac
    75241 gcaagggtag cccccatgtt catttcctct ggttagatgg cgactatgaa actattctcg
    75301 cgcgaatgca gcgtcgggct gggcatttta tgccggtagc gttactaaaa agtcagtttg
    75361 aggcactgga gcgtccacaa gacgatgaac aggatattgt gcgcattgat atcaaccatg
    75421 atattgccaa tgtcaccgaa caatgtcggc aggctgtgct ggcgatacga caaaacagaa
    75481 tatgtgcgaa agagggcagc gcctcagatc agcgctgcga atgattttca aaaatcggct
    75541 ttcaacacca cgcggtaacg cgccttaccg tcgcgcacat gctggatggc gtcgttaatt
    75601 ttcgacatcg ggaacagttc ggtagtcggc gcaaccttgc tgcgggcggc aaagcgcatc
    75661 agcttacgca actcataagg tgtaccggta gcagagccag agacactgcg atcgcccgca
    75721 atcagcgtaa aggccggaac cggcaacggc gtgagaaccg caccgacggt gtggaaatta
    75781 ccgccatatg tcagcgcctc aaaataaggc tgccagtcga ggctgacgtt cacagtattg
    75841 ataatgagat caaactgccc cgccagagtt ttcagtgcct gcggatcgcg gctattcacc
    75901 actttatcgg cacccatcgc cagtacttcc tgctctttcg ccggatttga actaaaggct
    75961 gtcacctcgc atcccattgc gtgcagaagt tttatagcga tatgtcccag cccgccaata
    76021 ccaattaccc caacgcggct ggtagcagtg atatggtgca tcaacagtgg tttaaagacc
    76081 gtgataccac cacacagcag cggcccggcg gactcgatat cgatattttc cggcagtggg
    76141 atcacccatt gccagtctgc acgcaacttc tcggcaaaac cgccgcgatt cataattgtt
    76201 ggcaccgcac cttgctcgca gttgatctga ttaccgctaa tacaggcatc gcaatgcccg
    76261 cagctacgcg ccgtccagcc aatcccgaca cgctgaccga cctgcaaacc tttatcctgc
    76321 gcggcgctcc cgagcgccac cacgcgacca atcacctcat gcccggcaat cagcggatat
    76381 tgtgaaaatc cccattcatt gtcgatcatc gatagatcgg aatggcagat cccgcaataa
    76441 tccacctgca cttcaacatc ttgtggcctc agctcaccgg gatcgtactc ataaacttcc
    76501 agttcgccgc ccgcttcttt tgcggcatag ctttttatca tcgacatttt ttggtccttc
    76561 tctggtgttg tctgggaagt gtagagcatg gcagggcggg gtgcctggag tgtgacaaag
    76621 gttacacatc gcggtatgca atgctgaaaa tttcagcact tagcgaggtg cgagcaagct
    76681 ggcgcttgca tggtggcgtg cgacaggtat aatccacaac gttttccgca tacctcttca
    76741 gtgccgaagt ggcgaaatcg gtagacgcag ttgattcaaa atcaaccgta gaaatacgtg
    76801 ccggttcgag tccggccttc ggcaccaaaa gtatgtaaat agacctcaac tgaggtcttt
    76861 ttttatgcct gaaatccagt gtttatctat ctttcccgct atattaactc tctcaaggtc
    76921 aaccgatatc aacgtacatc taccaacata tgttggtaca gatgatggta tttccggttc
    76981 gataatgctt gtaccaacag ggagggaata cgcatggcat taacagatat caaagtcaga
    77041 gcagccaagc caacggataa gcaatataag ctgactgatg gtggcggtat gcatctgctt
    77101 gtccatccaa atggttctaa gtactggcgt ttgcagtacc gttatgaggg aaagcaaaaa
    77161 atgctggcac ttggggttta tcctgaaatc acactagcgg atgccagagt acgtcgtgac
    77221 gaggcgcgta acctgcttgc gaatggcgtc gatccgggag acaaaaagaa aaatgataag
    77281 gttgaacaga gtaaagcacg aacctttaaa gaagtcgcga ttgagtggca tggcaccaat
    77341 aaaaagtggt ctgaagatca cgcccatcgt gtgctaaaaa gtcttgaaga taatcttttt
    77401 gcagcgcttg gtgaacgtaa tatcgctgag ttaaaaactc gagatttatt agcacctatt
    77461 aaggccgtag aaatgtctgg acgtcttgaa gtggccgctc gtcttcagca gcgcactaca
    77521 gccatcatgc gctatgcagt gcaaagtggg ttaattgatt ataacccggc acaagagatg
    77581 gctggggcgg ttgcttcctg taatcgacaa catcgtcccg cgcttgaatt aaagcgcatc
    77641 cctgagttgc ttacaaaaat agatagctat actggtaggc cgctaacccg atgggcgata
    77701 gaactcactt tgctgatctt tattcggtcc agtgagctgc gttttgctcg ttggtcagag
    77761 atcgatttcg aagcgtctat atggactatc ccaccggagc gggagcctat tcctggagtg
    77821 aaacattccc atagaggctc aaaaatgcgt acaacgcatc tagtgcctct ttcaacgcaa
    77881 gctcttgcaa ttttaaagca gataaaacag ttttatgggg cccatgactt gatatttatt
    77941 ggtgatcacg attcgcacaa acccatgagt gagaatacgg taaatagtgc gttacgggtc
    78001 atggggtatg atacaaaagt agaggtttgt ggtcatggct ttcgaacaat ggcctgtagt
    78061 tcattggtcg aatcaggttt gtggtctcgt gatgctgttg aacgtcagat gagccacatg
    78121 gagcgaaatt cagtgagggc cgcgtatatc cataaagcag agcatctgga agaacggcga
    78181 ttgatgctac agtggtgggc cgattttctg gatgtaaaca gagaaagatt tatcagtcca
    78241 tttgaatatg caaagattaa taatccatta aaacagtaat catcccgggc aaatgcccgg
    78301 gaattattct aggattattt tctttgttaa aaaagacaaa cggtattaac tgatgtattt
    78361 actatttacc gctccctgca ggggggatta tcagaatccg cggttattca ttatttatat
    78421 actttcaata aaaaagtaac ctcataaaaa atcctgtact cacagggtga tgcgggataa
    78481 ctgacgtaag gagtgctgca gggatttgat ggtcccttgt gctgatatga atacggatat
    78541 tttatacaga atacttttca gtacaagcga ctgtattaca gtctgccata tatgattacc
    78601 tgtgatcagg aagaataagt ggctgaggtt tcaaactgtc tgccggtata ttcctctctc
    78661 ccttaaaaac cataacagtt taattatctt cgtctgtcag cggattgaat ggcggtatat
    78721 tttcagtaca aataccggtc atccactgaa aaatacctgc gaaatgacgg gcactgattt
    78781 ttctactgac gggctgatga gacgttatgt cactgacata taatccctgc gcacactctc
    78841 accagtgaca ataacaatcg tatcatataa cggtgctccc ggctggattt tccagttgtc
    78901 agccccgtgc tgactcagtt gttcataacg ctgcatttca cgtaatgtgt cagttgtccc
    78961 cacaactgtt cctttaacca tccgcaatgg ccaacggttt acagagcata atacgaatag
    79021 ccgtagagcc agccagttac ggtggtcgcg atggtgtgtc cgccagaaaa tcaccacgaa
    79081 taccagaatc gcggcactga ccagaaaatg ataaacagga aacacggggc ggttcacttt
    79141 gctttctcct gtagtgtttt ctgtaggttc tgaaccggtt cgagcggggt tgtgaggctg
    79201 atggcaatcc ccgggctctt cctgcatgtt acactggtac tgcaagaggg ggccttcgct
    79261 cctctgacat tactcagtat tttcactact acaccccatc tgccatccat aacaaccgat
    79321 tcaccccctc gcgaggcgtt gttgcccgtc acccggacag acatcgttat ggacttctct
    79381 tgttgcacga tcgatcaatt gttatgcatg ctgacaccac aaccccggtg ggtatgaccg
    79441 atgcctttct cactctcttc ccggtcatcg tcggccttcc ccgttattgt ggagtgtcag
    79501 caccctcgac ggcatttcga tggctgctca gtgttcactc acgttacggc ccgcataacc
    79561 cgttgacttc ctgaagaagt cgtttcaggg agagattcag cccttttgtt acctcctgga
    79621 ccgcttcttg tccttccagt cggagcgaga gttagccggg gcggactttc accgacggaa
    79681 agatcgtgca tttcaaggca cacacaacaa tatctgcgag cgtgctatcc gtccggtcgt
    79741 tatgggacga aaggcctggt tgttcgctgg ttcactgatg gccgggaacc gcgcggcaca
    79801 gataatgagc cttctgggaa ccgcagtctg gagccacatg cttggctgac ggacgtcctg
    79861 acgcgtctgc cggagtggcc ggaggagagg ttggctgagt tactgcctct tgagggcttt
    79921 accttcttcg ggtaagtgat acctgccgtc aggtgtttgt gcaccgggcc ataacctgca
    79981 gttgggaatt gaacgcctga caacaggaaa tgagctagag cagtaaacac gcgggctgac
    80041 aatgggtcag aatatgagtc agcccggcgt tattcctggg gtcagtactt cagatccagc
    80101 ctccggtacc agactagcta gcccctacgc aaccaaataa atcaaccatc gtcagagtgc
    80161 aatcgttcat atacactgtc ggtgatgcgc tgaaaaatgc gttaatgttg agttcctcga
    80221 acccttacca attaagggac atagccagat acgcaactgg ctgatgcaag cagtctaatg
    80281 tctttagatc cgctggcctg cgggcatgac aatgtcaaac agtcaaagat tgtggcgttg
    80341 acggtcagtc ttggggacaa aaaagctcag tattctcgtt ggcagccaga taatttaaca
    80401 gttgatgctg aaacgaaata ggcatcttgg ttctggtgtt tgtaacaaac tatcagctac
    80461 aaaaatatgc tcaatttgtg acatcagtaa caaaacgcgt tttgttatgt ggattgcttg
    80521 ttttttgatc tggtataaca ggtataaagg tatacagaaa agcaaagaaa tactgcaaag
    80581 gaaaacagct ataacgtaag ctaaagtaat aacctctcag tctttcctca tttgactaag
    80641 ggagttttat tcaacctgaa cggactacga aaatgagcac aatgaataag tcaatttttg
    80701 aagcagggtt gaactgtgcc agtggtggaa tatctggcga acattgtttt ttgttggtga
    80761 cccaaaactg taggtgggta tcagttttat ctttcataga gtgaaatatg ttaagaagaa
    80821 atggaggaaa agattgtacg gattaggtat tgataacaat caatagtact ggcgtatttg
    80881 aagacaatat aattatttct ggatattgtt gaggctccct aatatttact ttaagggcta
    80941 tattagaata acacaggaaa caaatatggc aacagcatgg tataaacaag ttaatccacc
    81001 acaacggaaa gctctttttt ccgcatggct tggatatgta tttgatggct ttgattttat
    81061 gatgatattt tacattcttc atattataaa agcagatctt ggcattacgg atattcaggc
    81121 tactttaata gggacagtgg ccttcatagc cagacctatt ggaggtggtt tttttggtgc
    81181 catggctgat aaatatggtc gtaagccaat gatgatgtgg gcaattatca tttattcagt
    81241 cggaacaggc cttagcggta ttgctacaaa cttatatatg ctcgcagttt gccgttttat
    81301 tgttggctta gggatgtctg gtgaatatgc atgtgcttca acttatgcgg tagaaagttg
    81361 gcctaaaaat cttcaatcta aagctagtgc ttttttggta agtggttttt ctgttggaaa
    81421 tattattgcg gcacaaataa tccctcagtt tgctgaagta tatggatgga gaaactcttt
    81481 ttttataggc ctgttaccag ttttactagt tctttggatc agaaaaagtg ctccagaaag
    81541 tcaggagtgg attgaagata aatataagga taaatcaact tttttgtctg tcttcagaaa
    81601 accacatctt tcaatctcta tgatcgtttt cctcgtctgt ttttgtctat ttggtgcaaa
    81661 ctggccgata aacggactac ttccttccta cctggcagat aatggagtta atacagtggt
    81721 tatttcaact ctgatgacaa tagcaggttt aggaacactg acaggtacaa tattttttgg
    81781 ttttgttggt gataagattg gtgtaaaaaa agcctttgta gtcggtctaa taacttcatt
    81841 tgttttcctt tgtcctcttt tttttatttc tgtgaaaaac tcttctctta taggattatg
    81901 tctctttgga ttaatgttta caaatttagg tattgcaggg ttggttccaa aatttatata
    81961 tgattacttt ccaacaaaat taagaggatt agggaccggt cttatttata acttaggggc
    82021 aactggagga atggccgcac ctgtattagc tacatacatt tcaggatatt atggcttagg
    82081 tgtttcatta ttcattgtta cggttgcatt ctctgcctta ttaattttgt tagttggttt
    82141 tgatattcca ggtaaaattt ataaactatc cgtggctaaa taattggagg ctttatgatt
    82201 aattatggcg ttgttggtgt tggatacttt ggcgctgaat tagctcgttt tatgaatatg
    82261 catgataatg caaaaattac atgtgtatac gatcctgaaa atggagaaaa tattgcccgt
    82321 gaattgcagt gtatcaatat gtcaagcttg gatgctttag tctcaagtaa attagtcgat
    82381 tgcgtgatcg tagccacccc aaattatctg cataaagaac cagtaattaa agcagcaaag
    82441 aataagaagc atgttttttg tgaaaaacca attgcattaa gttatgaaga ttgtgtggat
    82501 atggtcaaag cgtgtaaaga agctggtgtg acctttatgg ccgggcatat tatgaatttt
    82561 ttcaatgggg ttcaatatgc acggaagtta attaaagaag gtgttatcgg cgaaatatta
    82621 tcatgtcata ctaagagaaa tggctgggaa aacaaacaag agagactttc ctggaaaaag
    82681 atgaaagaac aatctggtgg acatctatat catcatatac atgagttaga ttgtgttcag
    82741 catttacttg gagaaatacc agagacggtt actatgattg gtggaaattt ggcccattct
    82801 ggtccaggat ttggcaatga agatgatatg ttatttatga ccttggaatt cccgtcagga
    82861 aaactagcaa ccttagagtg ggggagtgca tttaactggc cggaacatta tgtcatcatc
    82921 aatggaacta aaggctctat taaaattgat atgcaagaaa cagcagggtc acttaggatt
    82981 ggcggtcaga caaagcattt tttggtccat gaaacacaag aagaagatga tgatcgtcgg
    83041 aaaggcaata tgacctcaga aatggatggc gctatagcat atggtcatcc aggaaaaaaa
    83101 acaccattat ggcttgccag tttaataaga aaggaaacgt tattcctcca taatatcctc
    83161 tgtggtgcaa aacctgaaga agattatatt gaccttctca atggtgaggc ggccatgtcg
    83221 gcgattgcta ctgctgatgc tgccactctt tcaagatcgc aggacaggaa agtgaaaatc
    83281 agtgagatca ttaaacatac atcagtaatg taaaaactat acgcctcttg atttaattca
    83341 ggaggccttt tatgtatgat caagaatttt atcttcgata atctcataat tttagcagtg
    83401 ccatttatga taaaaacatc ccttaaaacc aatctaatct ttttcttcct ctgcgtattt
    83461 gttccccgta tggcgtcata atttacgcta cgtaatacgg gatagttacg atacgcagcg
    83521 atagcgctaa gttttagtta aaatccccca tcagcgggat atggggatct ctgggaggtt
    83581 caggttttag catcgcgatt aatacgacag acgctggtga cgatgatgcc aagtagtggc
    83641 tcttccgaat aaggttccca ctgaccaatt ggcgcgcgat tcgcaataac atctcccttc
    83701 gcccagtatt gggaaaagta gatacattca aactgtgtac gctgtttcgt ctcacagttc
    83761 acgacattgc tggccgatga gctgaccatt gggcctatcc gggctgtccc gatggatatt
    83821 acgccgaagt atgtgggaat tgccagcgga ttgatgaacg ccggttccgc tgtcgccgac
    83881 attatttcac ctatcgcctt tggcattatt atcgataaaa caggcaactg gagtttacca
    83941 ttctacggtt ccgtcgcatt gcttgttatt ggtatattcc tgacgttctt catgcgtcca
    84001 gataagtcat tgtaaaagtc gaattcaggg ctgaagtggt ctactgaatt tggccatctg
    84061 aacagggtga tatgctcacc ttagaacaac acaggtgcac caatgaaaag aagaaatttc
    84121 agtcctgaat tcaaacgtga atccgctcag ttggttgtcg atcaaaatta cactgtttct
    84181 gacgctgcta aggctatgga tgttggtctt tccacgatga cggaatggtt caaacaactg
    84241 cgtgatgagc gtcagggcaa acgccaaaag ccttcctgat aacaccggaa caaatcgaac
    84301 tacgtgagct aaagaaaaag ctacaacgaa ttgaaatgga aacgaaatat taaaaaactt
    84361 tccatgtgtg aaaccccgtc ccgagtctca gaaactcccc ggtaaagctg gcagtctaat
    84421 atagcgaagg aagcagtcgt cggccttttg gtggtatgac ccttaaaaaa catggttcag
    84481 gggctgtggc ggatccgacg gtggtagctt gatcccgttc aggaaggtga tttctccgtc
    84541 gtctcctcct tcgttcatta aaaatgagca aggagattta tatgaaaaaa tcctcccgaa
    84601 tgtctattgt ccattctcat gctgcgggcg ttgatattgg ggccaaattt cacgttgtgg
    84661 ccgtaccgcc tgatgctgac gccgagccaa taaggacttt tcaaagtttc accggcgacc
    84721 ttcatcgtat gtctgactgg ctcaaaacct gtcatattac aaccgtcgcc atggagtcca
    84781 ccggcgttta ctggttgcct gcttttgaga tccttgaagc agcgggtttt aatatgatac
    84841 tcgttaatgt cagtgccgca aaaaatgttc ccggtcgcaa gactgatgtc aacgacgcac
    84901 agtggttgca gagacgacat gcatttggtc ttctccgggc cagtttcaga cctgtacacg
    84961 acatttcagt cctcaggtct tatctgaggc aacgagaacg cttgactgaa taccgggctg
    85021 cgcatattca gcatatgcag aaagcgctga tgcagatgaa tgtgcaattg catcatgccg
    85081 tatcggatat cactggcgtc accggtctct cgatcgtcag agctattgtc agcggtgagc
    85141 ccgatccttc agtattgatc cagtaccgtg atgtgagatg taagaagacg ccagaagtac
    85201 ttcagcaggc actgacaggg aactggcaac ctgagcatct ttttgcactt gagcagtcgg
    85261 tggcattttt ctatttttac caggaaaaga tccgtgaacg tgacgatcaa atcgaaattc
    85321 attaatacaa ctcagcaccg ggactgaaaa tcctgaagga gtattaccct gcgccagaca
    85381 tcgtacaaag caacccaatc agctttcctt gatgtcagac cgttactctg gaaaattacc
    85441 acggcagatc tgacacaaat acatggggtt gggccttatc tggcactaaa attcgttgcc
    85501 gaacgcggta ctgacatgaa tcgctggcct gatgcgagcc attttacctc gtggctattt
    85561 tgtccacggg aaataagatc tccggtggaa aagtgttgtc ctcgaaaacg agacgttcat
    85621 ccagttgaat tgctgctgca ctcaggctgg cagccacaac gattggtcgg agcgatactg
    85681 cgttgggtgc tttttatcgt cgattatcat cccgtatagg taaagcaaaa gcagtcactg
    85741 caacggcaag aaaactggca acactgttct acaatgcctt gaaatatggt aaaaaatatg
    85801 tcgacaattg tgctgattac tatgaagagc gttaccgtag ttgcgtactc aatggactta
    85861 aacgaagagt aaaatcactg ggttattctc tccagcaaga ccctgaatta tgagagtgaa
    85921 ccaggagttt ctcaggaagg ctaccgcgct cttgatgtca gattcctgaa cagtctcaat
    85981 aatcgggaaa ctcagagcgc attatcctgt gttcacactc tgtcatgtgt tcgcagttca
    86041 tcgctgcagc tacagatatt ggaaaacccg tcctgaaaaa acagacggca gatgggctag
    86101 gtaattgact tgcatggcgg agctaggagc attgccacaa tggcaacccg gagagattac
    86161 cagatgggac gctggcttgc tggcaggctc atgaaaaaac aggggctggt caactgtcag
    86221 ccgccgcccc accggtcatg aacatgtcgc tattcctaac tatcttgaac cgcacttcgc
    86281 tgtgacacag ctaaatcaag tgtgatgacg tgacgtatat ctggataggt aaacgatggg
    86341 gatacctcgc agtagtactt gctctgttcg caataaagcc cggggatggg caatgtcgtt
    86401 ctcaccggac agcagactga ctataaaagc gctgaagatg gccgaggaaa cgcgaggtaa
    86461 accagaggga gttatgttcc ttagcaacag gagagccatt acacaagtag acagttcagg
    86521 caggtgatgt ggcgttatcg gatcaaggag agtatgagtc gtcggggaaa ctgctgggat
    86581 attagcctaa tggtacgttt cttcagaagt ctgaagaacg agtgggcacc agtgactggt
    86641 tactttagcc ttaacgaagg tgtccataca ataacagagt atataattga atattaagtg
    86701 cactccggcc tcatgaatat aacggtggat tacctccgaa cgaatcggaa aatagatact
    86761 ggaaaaaatc tgaaattgtg accatttttt gttgaccact acaaataaat aaaagagttg
    86821 cagtgcttgc tcggtaagaa aacaatggaa gttgagatcc tcaaagaagc tgtggagtac
    86881 accactctac agtagttgcg caaatttgga gtggacgata atgtgatcgt ctataagggc
    86941 aacgctatca tagtcttgtc ctggcgggta aaaaagcgcg cttaccttaa cgataagcgc
    87001 gccgctgttc aggccttgag tggttattca attcctgtgg tgactgtaaa agtgcgcgtt
    87061 tgctgcggtg caacctgaat cagcgtgcca ttacgttgcg cggcaagata cccctcaggc
    87121 cgacaggttg caggtaatgc aaaggcggct acctgttgct ctccgttata aaggatccag
    87181 cgtgtcacat aatttagttc agcactgtag aaacgagtaa caaacgtagt gccatcggga
    87241 gagatcatgc gaaactctgg ctgatctgta taagcgtcca gtttgtctgc aaagaagaca
    87301 atttctggat cataaaattc cggttgactc agcgtcgaca gagaggcttc tccctgcata
    87361 atccgttgat taaacgccag ccactgagcg gtgggattaa catgcgaagg cactgattca
    87421 cgtaatctta atatttcgtc cgggatattc tggctgaatg tagcatttgg tatatatgca
    87481 taattcatgt ggcacatata ttgtagtggc atatctacag aagccagatt ggttacggcc
    87541 atcttaatat cgaacagtgt agaggatttg tgaaggacca ctgttggttg agccagataa
    87601 tgatgaccga aacccattac atactcgtaa cgcccgttaa ggcgtaacat atctccgtct
    87661 aattccagcc atgcttcatc catcgcggca caggccattt caccgtgtag cagatgagta
    87721 tcttccgcag atgggcagcc attagccagc aaacctgaat gaaaagcaaa acagccatag
    87781 gtctctatca cctctgtcgc cggtttaggc tggcgaaaca tattgcacat ggtgaggccg
    87841 tgtccatcaa attgcgcatc ccaaatcatc tgccccatcc agggaagaat aatcaaatgt
    87901 ccacgactgt ttgcaatttt aagcccctcg acaccgctgt catagcgaaa agacgtgaca
    87961 gtaaaatcac tattttccag caagatacga ggtttctcgc caaaaagcgc ccgccacaaa
    88021 ttaatacgcg tactcataac ggttctcctc aggacgctgt gacttcagcc agtgcggtac
    88081 gtactttgct ttcacgccag aagtagactc cgacatagac aaagcagagc atagaaacca
    88141 ggaatgaaag ctgtagtgag tggaacatat ctgcaatata tccctgaatt gccggaacca
    88201 ccgcggcacc gacaatagcc ataacaatga ctgctcctgc catttctgta tgttcgttat
    88261 caactgtatc cagtgttcct gcatagatcg tcgcccagca agggccaaac aaaacactta
    88321 ccaggacggc gacatagacc gcgctgaaac ttggagccag tgcaacatat gccaggaaca
    88381 gcgcccctat aacggaatag agaatcaata ctttttccgg attaaaacgc gtcataagga
    88441 tgttggctat aaacttgcca ataaagaagc aggcaaagct atagaccatg aagtttgaag
    88501 catcacgttc gttgatatcg cccaactcca gcgccagacg gatggtaaat gaccatactg
    88561 cgacctgcat acccacataa aggaactgcg ccacaatacc gcgacgaaag cgcggatttc
    88621 tagccagata gcgcagcgta tccattgctg acgggcgttt atagtgactt gtctgtgcca
    88681 ctttacaggt tgggaagcgg gttaaaagga acaacaccat gaccacaacc agaatcataa
    88741 tcatatactt atacggttca agggtgttct ctaacattag caccttaaag ttgtgaattt
    88801 gctcggcgtt cattccggac atctgcttct caaggctttc cccctcggag aaaaccagat
    88861 atttgcccaa taaaatacca gacgcagcac caatcggata aaaggtctgg ctgatattga
    88921 gccgcaatgt ggcataggct tttggaccga tcattgaact gtatgtgttc gctgcagttt
    88981 caaggaaact caggccaatc gcaatcgcaa aaatagctgc aaggaacata gtgtaggttg
    89041 ccatatgcga ggcagggaaa aaaagtgtac aaccaccaat atacagcgtc aggccaatta
    89101 aaattgccac cttataactg gtctttttaa tcacaaggga tgctggtatt gcaattaaaa
    89161 aataacctcc ataaaatgcg ctctgcacca atgctgaagc aaagttactt agggaaggtg
    89221 cgaataagcg gggaaattct tctcggctga ctcagtcatt tcatttcttc atgtttgagc
    89281 cgattttttc tcccgtaaat gccttgaatc agcctattta gaccgtttct tcgccattta
    89341 aggcgttatc cccagttttt agtgagatct ctcccactga cgtatcattt ggtccgcccg
    89401 aaacaggttg gccagcgtga ataacatcgc cagttggtta tcgtttttca gcaacccctt
    89461 gtatctggct ttcacgaagc cgaactgtcg cttgatgatg cgaaatgggt gctccacctt
    89521 ggcccggatg ctggctttca tgtattcgat gttgatggcc gttttgctct tgcgtggatg
    89581 ctgtttcaag gttcttacct tgccggggcg ctcggcgatc agccagtcca catccacctc
    89641 ggccagctcc tcgcgctgtg gcgccccttg gtagccggca tcggctgaga caaattgctc
    89701 ctctccatgc agcagattac ccagctgatt gaggtcatgc tcgttggccg cggtggtgac
    89761 taggctgtgg gtcaggccac tcttggcatc gacaccaatg tgggccttca tgccaaagtg
    89821 ccactgattg cctttcttgg tctgatgcat ctccggatcg cgttgctgct ctttgttctt
    89881 ggtcgagctg ggtgcctcaa tgatggtggc atcgaccaag gtgccttgag tcatcatgac
    89941 gcctgcttcg gccagccagc gattgatggt cttgaacaat tggcgggcca gttgatgctg
    90001 ctccagcagg tggcggaaat tcatgatggt ggtgcggtca ggcaaggcgc tatccaggga
    90061 taaccgggca aacagacgca tggaggcgat ttcgtacaga gcatcttcca tcgcgccatc
    90121 gctcaggttg taccaatgct gcatgcagtg aatgcgtagc atggtttcca gcggataagg
    90181 tcgccggcca ttaccagcct tggggtaaaa cggctcgatg acttccacca tgttttgcca
    90241 tggcagaatc tgctccatgc gggacaagaa aatctctttt ctggtctgac ggcgcttact
    90301 gctgaattca ctgtcggcga aggtaagttg atgactcatg atgaaccctg ttccatggct
    90361 ccagatgaca aacatgatct catatcaggg acttgttcgc accttccatc agagaccagg
    90421 gatatcgtgg cggaatgacc attctcaggg cgttcattcg ttctctctcg gtt
//
LOCUS       DAZFFE010000006        79758 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFFE010000006 DAZFFE010000000
VERSION     DAZFFE010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.006"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(117..404)
                     /gene="yebO"
                     /locus_tag="ACHJ69_000520"
     CDS             complement(117..404)
                     /gene="yebO"
                     /locus_tag="ACHJ69_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO5013582.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(479..622)
                     /gene="mgrB"
                     /locus_tag="ACHJ69_000521"
     CDS             complement(479..622)
                     /gene="mgrB"
                     /locus_tag="ACHJ69_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO5013583.1"
                     /translation="MKKFRWVALVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            781..1020
                     /gene="yobH"
                     /locus_tag="ACHJ69_000522"
     CDS             781..1020
                     /gene="yobH"
                     /locus_tag="ACHJ69_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO5013584.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1164..1955)
                     /gene="kdgR"
                     /locus_tag="ACHJ69_000523"
     CDS             complement(1164..1955)
                     /gene="kdgR"
                     /locus_tag="ACHJ69_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO5013585.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     MQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2132..3505
                     /gene="yebQ"
                     /locus_tag="ACHJ69_000524"
     CDS             2132..3505
                     /gene="yebQ"
                     /locus_tag="ACHJ69_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5013586.1"
                     /translation="MPKVQADGLPLPLRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLIAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3551..4432)
                     /gene="htpX"
                     /locus_tag="ACHJ69_000525"
     CDS             complement(3551..4432)
                     /gene="htpX"
                     /locus_tag="ACHJ69_000525"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO5013587.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATGLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4624..6672)
                     /gene="prc"
                     /locus_tag="ACHJ69_000526"
     CDS             complement(4624..6672)
                     /gene="prc"
                     /locus_tag="ACHJ69_000526"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO5013588.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLESDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDSTRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6692..7390)
                     /gene="proQ"
                     /locus_tag="ACHJ69_000527"
     CDS             complement(6692..7390)
                     /gene="proQ"
                     /locus_tag="ACHJ69_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO5013589.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRSQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7487..7984)
                     /gene="msrC"
                     /locus_tag="ACHJ69_000528"
     CDS             complement(7487..7984)
                     /gene="msrC"
                     /locus_tag="ACHJ69_000528"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO5013590.1"
                     /translation="MNKAEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8114..9397
                     /gene="yebS"
                     /locus_tag="ACHJ69_000529"
     CDS             8114..9397
                     /gene="yebS"
                     /locus_tag="ACHJ69_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO5013591.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGAMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9366..11999
                     /gene="yebT"
                     /locus_tag="ACHJ69_000530"
     CDS             9366..11999
                     /gene="yebT"
                     /locus_tag="ACHJ69_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO5013592.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLSSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPGGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12079..13518
                     /gene="rsmF"
                     /locus_tag="ACHJ69_000531"
     CDS             12079..13518
                     /gene="rsmF"
                     /locus_tag="ACHJ69_000531"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO5013593.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLQLTAPYGWTLTPIPWCEEGFWIERDSEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYHDAVEFLPLGD
                     LFPGANKALTEDGFLHVFPQIYDCEGFFVARLRKTQAIPVLPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13636..13872
                     /gene="yebV"
                     /locus_tag="ACHJ69_000532"
     CDS             13636..13872
                     /gene="yebV"
                     /locus_tag="ACHJ69_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO5013594.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13977..14168
                     /gene="yebW"
                     /locus_tag="ACHJ69_000533"
     CDS             13977..14168
                     /gene="yebW"
                     /locus_tag="ACHJ69_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO5013595.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14169..14825)
                     /gene="pphA"
                     /locus_tag="ACHJ69_000534"
     CDS             complement(14169..14825)
                     /gene="pphA"
                     /locus_tag="ACHJ69_000534"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO5013596.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASRQMSLWLINGGD
                     WFIALADNQQKQAKTALEKCQHLPFILELHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGIAGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15220..15561)
                     /gene="yebY"
                     /locus_tag="ACHJ69_000535"
     CDS             complement(15220..15561)
                     /gene="yebY"
                     /locus_tag="ACHJ69_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO5013597.1"
                     /translation="MMKNSILAFLLLTSSAAALAAPQVITVSRFEVGKEKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15574..16446)
                     /gene="copD"
                     /locus_tag="ACHJ69_000536"
     CDS             complement(15574..16446)
                     /gene="copD"
                     /locus_tag="ACHJ69_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO5013598.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGAGWSDVFSPNIWLAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16450..16824)
                     /gene="yobA"
                     /locus_tag="ACHJ69_000537"
     CDS             complement(16450..16824)
                     /gene="yobA"
                     /locus_tag="ACHJ69_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO5013599.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16963..17193
                     /gene="holE"
                     /locus_tag="ACHJ69_000538"
     CDS             16963..17193
                     /gene="holE"
                     /locus_tag="ACHJ69_000538"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO5013600.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17295..17951
                     /gene="yobB"
                     /locus_tag="ACHJ69_000539"
     CDS             17295..17951
                     /gene="yobB"
                     /locus_tag="ACHJ69_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO5013601.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSYGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17974..18636
                     /gene="exoX"
                     /locus_tag="ACHJ69_000540"
     CDS             17974..18636
                     /gene="exoX"
                     /locus_tag="ACHJ69_000540"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO5013602.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18633..20693)
                     /gene="ptrB"
                     /locus_tag="ACHJ69_000541"
     CDS             complement(18633..20693)
                     /gene="ptrB"
                     /locus_tag="ACHJ69_000541"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO5013603.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIRLEGYTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     QSAD"
     gene            complement(20902..21561)
                     /gene="yebE"
                     /locus_tag="ACHJ69_000542"
     CDS             complement(20902..21561)
                     /gene="yebE"
                     /locus_tag="ACHJ69_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO5013604.1"
                     /translation="MANWLNQLQSLLGQSRSSTSSSADQGLGKLLVPGALDGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEKGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(21888..22244)
                     /gene="yebF"
                     /locus_tag="ACHJ69_000543"
     CDS             complement(21888..22244)
                     /gene="yebF"
                     /locus_tag="ACHJ69_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO5013605.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22311..22601)
                     /gene="yebG"
                     /locus_tag="ACHJ69_000544"
     CDS             complement(22311..22601)
                     /gene="yebG"
                     /locus_tag="ACHJ69_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO5013606.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22735..23913
                     /gene="purT"
                     /locus_tag="ACHJ69_000545"
     CDS             22735..23913
                     /gene="purT"
                     /locus_tag="ACHJ69_000545"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO5013607.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSENLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23969..24610)
                     /gene="kdgA"
                     /locus_tag="ACHJ69_000546"
                     /gene_synonym="eda"
     CDS             complement(23969..24610)
                     /gene="kdgA"
                     /locus_tag="ACHJ69_000546"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO5013608.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24647..26458)
                     /gene="edd"
                     /locus_tag="ACHJ69_000547"
     CDS             complement(24647..26458)
                     /gene="edd"
                     /locus_tag="ACHJ69_000547"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO5013609.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDNNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26693..28168)
                     /gene="zwf"
                     /locus_tag="ACHJ69_000548"
     CDS             complement(26693..28168)
                     /gene="zwf"
                     /locus_tag="ACHJ69_000548"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO5013610.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28506..29375
                     /gene="hexR"
                     /locus_tag="ACHJ69_000549"
                     /gene_synonym="yebK"
     CDS             28506..29375
                     /gene="hexR"
                     /locus_tag="ACHJ69_000549"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO5013611.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGTKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29503..30945
                     /gene="pyk"
                     /locus_tag="ACHJ69_000550"
     CDS             29503..30945
                     /gene="pyk"
                     /locus_tag="ACHJ69_000550"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO5013612.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQEAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31077..32048)
                     /gene="lpxM"
                     /locus_tag="ACHJ69_000551"
                     /gene_synonym="msbB"
     CDS             complement(31077..32048)
                     /gene="lpxM"
                     /locus_tag="ACHJ69_000551"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO5013613.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMVMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32167..33489)
                     /gene="mepM"
                     /locus_tag="ACHJ69_000552"
     CDS             complement(32167..33489)
                     /gene="mepM"
                     /locus_tag="ACHJ69_000552"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO5013614.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33505..34437)
                     /gene="znuA"
                     /locus_tag="ACHJ69_000553"
     CDS             complement(33505..34437)
                     /gene="znuA"
                     /locus_tag="ACHJ69_000553"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO5013615.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHDDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDDNLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            34516..35271
                     /gene="znuC"
                     /locus_tag="ACHJ69_000554"
     CDS             34516..35271
                     /gene="znuC"
                     /locus_tag="ACHJ69_000554"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO5013616.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35268..36053
                     /gene="znuB"
                     /locus_tag="ACHJ69_000555"
     CDS             35268..36053
                     /gene="znuB"
                     /locus_tag="ACHJ69_000555"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO5013617.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(36198..37208)
                     /gene="ruvB"
                     /locus_tag="ACHJ69_000556"
     CDS             complement(36198..37208)
                     /gene="ruvB"
                     /locus_tag="ACHJ69_000556"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO5013618.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37217..37828)
                     /gene="ruvA"
                     /locus_tag="ACHJ69_000557"
     CDS             complement(37217..37828)
                     /gene="ruvA"
                     /locus_tag="ACHJ69_000557"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO5013619.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKISRPDASSETLIREALRAAL"
     gene            complement(37967..38032)
                     /gene="yobI"
                     /locus_tag="ACHJ69_000558"
     CDS             complement(37967..38032)
                     /gene="yobI"
                     /locus_tag="ACHJ69_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFO5013620.1"
                     /translation="MCIFITHFFAEYVIFKYLLPI"
     gene            38106..38708
                     /gene="yebB"
                     /locus_tag="ACHJ69_000559"
     CDS             38106..38708
                     /gene="yebB"
                     /locus_tag="ACHJ69_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO5013621.1"
                     /translation="MNINYPAEYEIGDIVFTCISAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIEMFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAVGVETPQPELTEAV"
     gene            complement(38710..39231)
                     /gene="ruvC"
                     /locus_tag="ACHJ69_000560"
     CDS             complement(38710..39231)
                     /gene="ruvC"
                     /locus_tag="ACHJ69_000560"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO5013622.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(39266..40006)
                     /gene="yebC"
                     /locus_tag="ACHJ69_000561"
     CDS             complement(39266..40006)
                     /gene="yebC"
                     /locus_tag="ACHJ69_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5013623.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(40035..40487)
                     /gene="nudB"
                     /locus_tag="ACHJ69_000562"
     CDS             complement(40035..40487)
                     /gene="nudB"
                     /locus_tag="ACHJ69_000562"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO5013624.1"
                     /translation="MTDMAYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDASAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(40480..42252)
                     /gene="aspS"
                     /locus_tag="ACHJ69_000563"
     CDS             complement(40480..42252)
                     /gene="aspS"
                     /locus_tag="ACHJ69_000563"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO5013625.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILERTGAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANLTALAELSIQVVKKAENN"
     gene            42562..43128
                     /gene="yecD"
                     /locus_tag="ACHJ69_000564"
     CDS             42562..43128
                     /gene="yecD"
                     /locus_tag="ACHJ69_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO5013626.1"
                     /translation="MPELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            43125..43943
                     /gene="yecE"
                     /locus_tag="ACHJ69_000565"
     CDS             43125..43943
                     /gene="yecE"
                     /locus_tag="ACHJ69_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO5013627.1"
                     /translation="MIYIGLPQWSHPKWVRLGITNLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKEEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTAKNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            43996..44391
                     /gene="yecN"
                     /locus_tag="ACHJ69_000566"
     CDS             43996..44391
                     /gene="yecN"
                     /locus_tag="ACHJ69_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO5013628.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            44432..45175
                     /gene="cmoA"
                     /locus_tag="ACHJ69_000567"
     CDS             44432..45175
                     /gene="cmoA"
                     /locus_tag="ACHJ69_000567"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO5013629.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            45172..46143
                     /gene="cmoB"
                     /locus_tag="ACHJ69_000568"
     CDS             45172..46143
                     /gene="cmoB"
                     /locus_tag="ACHJ69_000568"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO5013630.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(46308..48737)
                     /gene="torZ"
                     /locus_tag="ACHJ69_000569"
     CDS             complement(46308..48737)
                     /gene="torZ"
                     /locus_tag="ACHJ69_000569"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO5013631.1"
                     /translation="MTLTRREFIKHSGIAAGTLVVTSAEPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPICSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGAFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPSGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAAQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYIRYGDFRADPVK
                     NALGTPSGKIEIYSRTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKLGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNALK
                     LTAFDQPAVQA"
     gene            complement(48762..49862)
                     /gene="torY"
                     /locus_tag="ACHJ69_000570"
     CDS             complement(48762..49862)
                     /gene="torY"
                     /locus_tag="ACHJ69_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO5013632.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKDGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGDLATVTPGTD
                     LTVVDVSGKQPIVRLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALIDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(50250..50996)
                     /gene="cutC"
                     /locus_tag="ACHJ69_000571"
     CDS             complement(50250..50996)
                     /gene="cutC"
                     /locus_tag="ACHJ69_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO5013633.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     FVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGITRVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIECHQAK"
     gene            complement(51010..51576)
                     /gene="yecM"
                     /locus_tag="ACHJ69_000572"
     CDS             complement(51010..51576)
                     /gene="yecM"
                     /locus_tag="ACHJ69_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO5013634.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            51792..53525
                     /gene="argS"
                     /locus_tag="ACHJ69_000573"
     CDS             51792..53525
                     /gene="argS"
                     /locus_tag="ACHJ69_000573"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO5013635.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(53578..53970)
                     /gene="flhe"
                     /locus_tag="ACHJ69_000574"
     CDS             complement(53578..53970)
                     /gene="flhe"
                     /locus_tag="ACHJ69_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO5013636.1"
                     /translation="MRALLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIPAAEPL
                     RFIWEVPGGGRLIPPLKIQRNEVIVNYR"
     gene            complement(53970..56048)
                     /gene="flhA"
                     /locus_tag="ACHJ69_000575"
     CDS             complement(53970..56048)
                     /gene="flhA"
                     /locus_tag="ACHJ69_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO5013637.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEAKPVNMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(56041..57189)
                     /gene="flhB"
                     /locus_tag="ACHJ69_000576"
     CDS             complement(56041..57189)
                     /gene="flhB"
                     /locus_tag="ACHJ69_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO5013638.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     AIISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(57398..58042)
                     /gene="cheZ"
                     /locus_tag="ACHJ69_000577"
     CDS             complement(57398..58042)
                     /gene="cheZ"
                     /locus_tag="ACHJ69_000577"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO5013639.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSIAGVVASQDQVDDLLDSLGF"
     gene            complement(58053..58442)
                     /gene="cheY"
                     /locus_tag="ACHJ69_000578"
     CDS             complement(58053..58442)
                     /gene="cheY"
                     /locus_tag="ACHJ69_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO5013640.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(58457..59506)
                     /gene="cheB"
                     /locus_tag="ACHJ69_000579"
     CDS             complement(58457..59506)
                     /gene="cheB"
                     /locus_tag="ACHJ69_000579"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO5013641.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSVPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(59509..60369)
                     /gene="cheR"
                     /locus_tag="ACHJ69_000580"
     CDS             complement(59509..60369)
                     /gene="cheR"
                     /locus_tag="ACHJ69_000580"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO5013642.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLAETLSTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(60484..60614)
                     /locus_tag="ACHJ69_000581"
                     /pseudo
     CDS             complement(60484..60614)
                     /locus_tag="ACHJ69_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
     gene            complement(60660..62321)
                     /gene="tar"
                     /locus_tag="ACHJ69_000582"
     CDS             complement(60660..62321)
                     /gene="tar"
                     /locus_tag="ACHJ69_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO5013643.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(62466..62969)
                     /gene="cheW"
                     /locus_tag="ACHJ69_000583"
     CDS             complement(62466..62969)
                     /gene="cheW"
                     /locus_tag="ACHJ69_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO5013644.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(62990..64954)
                     /gene="cheA"
                     /locus_tag="ACHJ69_000584"
     CDS             complement(62990..64954)
                     /gene="cheA"
                     /locus_tag="ACHJ69_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO5013645.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQETDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPDDIAEDDITAVLCFVIEA
                     DQITFETVDVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(64959..65885)
                     /gene="motB"
                     /locus_tag="ACHJ69_000585"
     CDS             complement(64959..65885)
                     /gene="motB"
                     /locus_tag="ACHJ69_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO5013646.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIALVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKHAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(65882..66769)
                     /gene="motA"
                     /locus_tag="ACHJ69_000586"
     CDS             complement(65882..66769)
                     /gene="motA"
                     /locus_tag="ACHJ69_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO5013647.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLEFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(66896..67474)
                     /gene="flhC"
                     /locus_tag="ACHJ69_000587"
     CDS             complement(66896..67474)
                     /gene="flhC"
                     /locus_tag="ACHJ69_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO5013648.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(67477..67827)
                     /gene="flhD"
                     /locus_tag="ACHJ69_000588"
     CDS             complement(67477..67827)
                     /gene="flhD"
                     /locus_tag="ACHJ69_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO5013649.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            68607..69035
                     /gene="uspC"
                     /locus_tag="ACHJ69_000589"
     CDS             68607..69035
                     /gene="uspC"
                     /locus_tag="ACHJ69_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO5013650.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRNVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKHH
                     FDLVICGNHNHSFFSRASCSAKRVITSSEVDVLLVPLTGD"
     gene            complement(69042..70466)
                     /gene="otsA"
                     /locus_tag="ACHJ69_000590"
     CDS             complement(69042..70466)
                     /gene="otsA"
                     /locus_tag="ACHJ69_000590"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO5013651.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNKFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTACGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(70441..71241)
                     /gene="otsB"
                     /locus_tag="ACHJ69_000591"
     CDS             complement(70441..71241)
                     /gene="otsB"
                     /locus_tag="ACHJ69_000591"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO5013652.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDTILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPKGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(71408..72394)
                     /gene="araH"
                     /locus_tag="ACHJ69_000592"
     CDS             complement(71408..72394)
                     /gene="araH"
                     /locus_tag="ACHJ69_000592"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO5013653.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(72409..73923)
                     /gene="araG"
                     /locus_tag="ACHJ69_000593"
     CDS             complement(72409..73923)
                     /gene="araG"
                     /locus_tag="ACHJ69_000593"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO5013654.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQIKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAITAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(73993..74982)
                     /gene="araF"
                     /locus_tag="ACHJ69_000594"
     CDS             complement(73993..74982)
                     /gene="araF"
                     /locus_tag="ACHJ69_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO5013655.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKASDIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            75779..76282
                     /gene="ftnB"
                     /locus_tag="ACHJ69_000595"
     CDS             75779..76282
                     /gene="ftnB"
                     /locus_tag="ACHJ69_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO5013656.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(76361..76612)
                     /gene="yecJ"
                     /locus_tag="ACHJ69_000596"
     CDS             complement(76361..76612)
                     /gene="yecJ"
                     /locus_tag="ACHJ69_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO5013657.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(76727..76813)
                     /gene="azuC"
                     /locus_tag="ACHJ69_000597"
     CDS             complement(76727..76813)
                     /gene="azuC"
                     /locus_tag="ACHJ69_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO5013658.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            77076..77399
                     /gene="yecR"
                     /locus_tag="ACHJ69_000598"
     CDS             77076..77399
                     /gene="yecR"
                     /locus_tag="ACHJ69_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO5013659.1"
                     /translation="MRLLILTLSLITLTGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HARTARYVSRGIADRACQQAGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            77571..78068
                     /gene="ftnA"
                     /locus_tag="ACHJ69_000599"
     CDS             77571..78068
                     /gene="ftnA"
                     /locus_tag="ACHJ69_000599"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO5013660.1"
                     /translation="MLKPEMIKKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(78106..78345)
                     /gene="yecH"
                     /locus_tag="ACHJ69_000600"
     CDS             complement(78106..78345)
                     /gene="yecH"
                     /locus_tag="ACHJ69_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO5013661.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            78536..79747
                     /gene="tyrP"
                     /locus_tag="ACHJ69_000601"
     CDS             78536..79747
                     /gene="tyrP"
                     /locus_tag="ACHJ69_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO5013662.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQLATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
BASE COUNT        19247 a        20852 c        19347 g        20312 t
ORIGIN      
        1 attatttgaa gtcagaccag agttttaatt tgaagcagct gcaccatgac agtgcgtata
       61 atcagcatga attgagtaac ccttcaacga cggggttact caagattgag gaaggattat
      121 cgttcggcga ccagacgaat aatatcttcg tcttcaaccg atttttgact ctcaacggtc
      181 tttttatctg ctttctcagg ttcgtttgct tcgcacaaac gacgtaacag tgcattttga
      241 cgtttttgct gatccagcaa cgcctcaagc agttcaatct gttcgttagt ccgtgaactg
      301 gcacgattga taaaaaacca caagatgagc ccgataagaa gaaccaccac cgatacaacc
      361 aaagacgcaa tgttcatcac gcctgaattt acaacttcgt tcatttcacc acctctgagt
      421 agaggcgcta ttctaccact gctggagagg aagaaaatct agtgctgaaa aaatgatatc
      481 accacgggat aaactggtta atggcacaaa ttccgctgaa aaattgtaca tcctgatcgc
      541 acatcatgtt gaatacctgc gcccaaagca gcaagcaagc caacaccacg acaaccagag
      601 cgacccatcg aaactttttc actccaatct ccgtttcacc taccttatgt cattaaacta
      661 gcatgcgtta gttaaacagc gcctaactat gtcgatattc gtgctttttc ggaatgagtc
      721 gcttgcttca gcgcatattg ccgctacgat taagcgaaca taaaaagaga agaggttgta
      781 atgcgattca tcattcgcac agttatgctg atagcgctgg tatggatagg attattactc
      841 agcggatatg gcgttttgat tggaagcaaa gagaatgccg cgggattagg tttacaatgt
      901 acctatctga ccgccagagg caccagcacg gtacaatacc tgcacactaa aagtggtttt
      961 ctggggataa cagattgccc gttgttacgt aaaagcaata ttgtggtcga taatggttaa
     1021 gggtaataaa taaccagatt ctcactcatg agtaaaatat tcttatcgca agtgcgttta
     1081 atataacgcc accttcaact ttatttttct catgctaaat aaagaacctg tctgtttgat
     1141 attcagacag gttcttactc tcatcagaac ggatagtcgt gataacccat ttgggcagaa
     1201 attttgcgcg ccgcggtatg caacattgcg acatattcct gtaaacgctc ttcagagaaa
     1261 cgcaacgtcg ggaaggagat gctcagaccg gcaatgacca cgccaaagcg atcaaatacc
     1321 ggaaccgcaa tgcaacgcag tccttcttcc tgctcttcat tatcttcgcc atacccctgc
     1381 tcgcgcacct ggtccagaac gggtaataac gcttctgtac tggtgatggt ccgctcggta
     1441 ctgcgtttat actccacgcc ctcaagaatt tgcatcactt catcgcgatc gcgccatgcc
     1501 agcagtacct taccaatcgc ggtgctgtac agcggattac gacgcccaat ccgtgaatac
     1561 atgcgcaaat tgtacattga gtcaattttg tggatgtaaa caatactgtc ttcgtccagt
     1621 gcgccgaggt ggatagtttc tttggtcagg cgggagatct cacgcatctg gatatctgcg
     1681 ctacgaatta aatcgacgtt ttgcaacgcg cgagcgccca gttcaaacaa tttcagggtc
     1741 agcgaatatt tctccgactc cccttcctgc gccacataac ctaaggtttt catggtctgt
     1801 aaaaagcgat aaacggtgct ttttgacatc atgacgcgct gcgacaactc ggttatccct
     1861 atttcgcgct cttcacccag cgcctgcaaa atgccaaaaa cttttagcac ggaagataca
     1921 gaatcaggct gtttatccag atctgcgtta gccatttatc acctcattgc gagtgtttta
     1981 taaaaatcag aactgttttt tattataatt tcgcaccagg gtggtcgcaa tccatctttt
     2041 gccggttagt tacaattctg cgacatccac cgtgaatatc agtgctagaa tcagacccct
     2101 gttgattatt caccaaagat ataaaattcc tatgccaaaa gttcaggccg acggcctgcc
     2161 attgcccctg cgatacggtg cgatattaac cattgtgatt ggtatttcga tggctgtcct
     2221 tgacggcgca atcgccaacg tcgccttgcc aacaatcgct acggaccttc atgccacgcc
     2281 ggccagttcc atctgggtag tgaacgccta tcaaatcgcc attgtcatct ccctgctctc
     2341 gttttcgttt ctgggcgata tgtttggcta tcgacgtatt tataaatgcg gtctggtcgt
     2401 ttttctgttg tcttcactgt tctgcgccct ttctgattcg ctgcaaatgc tcacccttgc
     2461 gcgtgtcata caaggtttcg gcggtgcagc gttgatgagc gttaataccg cacttatccg
     2521 cctgatctat ccacaacgtt ttctgggtag agggatgggc ataaactcgt ttattgttgc
     2581 cgtctcttct gctgccgggc cgacaattgc tgcagcaatc ctctccatcg catcctggaa
     2641 atggttattt ttaatcaacg taccgttggg tattatcgcc ctgcttctgg cgatgcgttt
     2701 tctgccaccc aatggttctc gcgccagtaa accccgtttc gacctgccca gcgccgtgat
     2761 gaacgcgtta accttcggcc tgcttatcac tgcgttgagt ggtttcgctc aggggcaatc
     2821 gctgacattg attggtgcgg aactggtggt aatggttgtc gttggtattt tctttattcg
     2881 ccgccagctt tctcttcccg taccgctgct accggtggat ttactgcgta tcccgctgtt
     2941 ttcactttct atttgcacat ctgtttgctc tttctgcgca caaatgctgg caatggtttc
     3001 cctgcccttt tacctgcaaa ccgtgctcgg gcgtagtgaa gtcgaaacag gtttacttct
     3061 gacaccgtgg ccgttagcaa cgatggtgat ggctccactg gcaggctatt tgattgaacg
     3121 cgtacatgca ggattgctgg gtgctttagg gttattcatc atggctgcgg ggcttttttc
     3181 cctggttctg ctgccagcgt cacctgcgga tatcaatatt atctggccga tgatcttatg
     3241 tggtgctgga tttggcttgt tccagtcacc caataaccac accattatta cctccgctcc
     3301 tcgcgaacgt agcggtggag ccagtggcat gttagggaca gctcgtcttc tgggtcagag
     3361 tagcggtgcg gctctggtag cgctgatgct aaatcagttt ggtgataatg gtacgcacgt
     3421 ctcgctgata gctgcggcta ttctggcagt gattgctgcc tgtgtcagtg gtttacgtat
     3481 cactcagcca cgatccaggg cataaaaaaa gcgcgtcgat caggacgcgc tttttagtat
     3541 ttacttcata ttacttcagg tattcacccg tacgcagagc ttcaatacgt ttatccagcg
     3601 gcgggtgagt catgaacaac tcactgagcg atttcgactt cccgttaatg cagaaagcca
     3661 tcatgctggt tgcttcttgc ggttcatagc tggttttcag gcgttgcagc gcggcaatca
     3721 ttttctcgcg accaaccagt tttgctgaac ccgcatcggc gtggaattca cgatgacgcg
     3781 agaaccacat ggttataatg ctcgccagaa taccaaacac cagttccaga cccgttgcaa
     3841 ccgcaaagta gatcagcggg ttgccgttgc tctcttcacc ttcatcacgg tttccgccca
     3901 taaaacccgc ggcaagctgc gccagaatac gggaaataaa gataacgaag gtgttcacca
     3961 cgccctgaat cagcgtcatg gtgaccatat caccattggc gatgtggctg atttcgtgag
     4021 caattaccgc ctcggcttca tccgggctca tgttctgcag caaaccggtg ctgacagcaa
     4081 ccagagaggc atcacggcgc gcaccggttg caaaagcatt gatgtccggc gcatggtaga
     4141 tagccacttg cggcatagcg atccccgcct gacgagcctg ggttgctaca gtattgacca
     4201 gccaacgttc cctttcgtta cgcggttgct cgatcacttc cccgccaaca gatcgtaatg
     4261 ccatccattt ggacatcaga agcgaaacga aggaaccacc aaaaccgaac agcaaggcca
     4321 tgatcatcag cccctgaacg ctgctcgact gtatccctgt caggctcagt accagcccga
     4381 aaacgaccat tacggccagg ttcgttagca ggaagagcgc gattcgcatc ataattttct
     4441 ttttacctca gtttaacaaa acgcaatatg cgatacccac atcgtatggg ttacgcgact
     4501 attttcaagt ctggatagtg cgtaagtcac cagaaagaca caactttaca ttttgtagca
     4561 tctgatttac ggcatcttgc cgctgttaaa aaatcaggca caatttcttg tgcctgattg
     4621 atattacttg acgggagcgg gttgttccgc gggtctggct ttttcaagct tcgccagatc
     4681 gagtgcgata ttcaccgtct catccagata aggatctggc tcctggtaat ctttcggtag
     4741 atcatccagt ttcttcaact ccggtttacc ttcgcgttta aagcgttcgt tcaaacgcgc
     4801 cagacgcgtc gaatcatctt cattattctc tttctcacgc acagcgtaat tcagagaaac
     4861 gatattgcgc ttgtccttca tagcgttgaa gcgcgcgata tccttcatga tgttctggaa
     4921 ctcaggatct ttcgcgatac gcgcattatg ttccttcagc agctccggtt caaaggccgt
     4981 taaatctcct gatttcacat aagtcgcggc atcaatgcta tcccacggca gcgcgttatc
     5041 ttcgaatttc tcacccgttt ccgtttcttc attacccgtc ggcatgatga tgtctggcgt
     5101 tacaccttta cgttgcgtac tgccgccgtt aacgcgatag aatttctgga tcgtgtactg
     5161 cacagaaccc agcgctggcc attcaggacg taacatctga tcgtaaatac ggttcaatga
     5221 acggtactgc tgaacggtgc ctttaccaaa tgtcggttca cccacaacca gggcacgacc
     5281 gtaatcctgc attgccgcgg caaagatttc tgaagccgaa gcactgaagc ggtcaaccag
     5341 caccaccagc gggcctttat agaagacctg tccgtcggtg tcgctgtctt cacgaacctt
     5401 gccgttgtta tcgcggacct gaacaatcgg gccggatgga atgaacagac cggagagcga
     5461 tacggcttca gtcaacgccc caccaccatt gctacgcagg tcgataatca cactgctgac
     5521 attctgtttt tccagtttct gcagttgcac tttgacatcg tctgtcaaac ctacatagaa
     5581 gcccggaata tccagcacgc cgactttctc tttaccgacg gtcttcaccg acattttaac
     5641 cgcgcggtct tcgagacgaa tacgttcacg ggtcaacgtt acagtacgcg tcttggtccc
     5701 tttaccagca ggtaaaattt ccagacgaac tttactgccc ttaggccctt taattaaggc
     5761 caccacatca tcaagacgcc agccaatcac gtcaaccatc ggcttgcctg tttgaccaac
     5821 accgacaatt ttgtcaccaa cgctgatagc tttgctcttc gctgccggac ctcctgccac
     5881 catcgaattg ataacggtgt agtcatcatc catttgcagc actgcgccaa taccttccag
     5941 cgacaaactc atttcagtgt tgaactgttc ggtattacgc ggggaaagat agttggtatg
     6001 cgggtcgatt tcacgcgcaa acgccgtcat tgccagcgag aaaacatctt cgctgttggt
     6061 ttgcgccaga cgacgaatgg caaatttgta gcggcgagtc agggtttcac gaatttcctt
     6121 atccgttttt cctgtcagct tcaggcttaa ctcgtcgaat ttgactttac tgtcccacag
     6181 cgcgttcaac tcagcctcgt ttttcggcca gggcgctttg ctgcggtcaa ggttataagt
     6241 gtcgttgccg gtgaaatcca tcggcttttc cagtaccgac aaagcgtact ggtaacgttc
     6301 aaaacggcgc ttttgcgcca gattgtagag atcgtagaaa acgtcgagtt tacctgaacg
     6361 cagttcatcg cctaactcag ttttcttttt cgcgaactgt tcaacatcgc tttccagcag
     6421 cacattgtgg ctgtaatcga gcagattcag gtagcggtca aagattttgg ccgaaaatgc
     6481 ctgatcgagg tcgaactggc gataatgaga acgggtgaag cgcgacgtta cgcgctcact
     6541 caccgtcgca tgctgcgtct cttcctttaa taccggaatt tgatcagcac gcgtgatatc
     6601 ttctacagcg aaggtctggc ctgctattgc aagcaggcca gctaacgcgg taagcctaaa
     6661 aaacatgttc atgcctggcc cggcctccgt ttcagaacac caggtgttct gcgcgcacaa
     6721 tcaaagacat acccgaattc agctggacgc ggacgccgtc tttggtgatt tctaatacgg
     6781 tggcatccat cgcgttttga cccgctttca ccttcagggc ttgtccgaca gtcagagctg
     6841 aaatgtcaga aaccggggtg tgctgttctt cgcgaggtgc ttttactgtt tttggcgctt
     6901 tctctaccgg cttttgcgaa cgaggtttac gttcagcgcc ttctttgcgg cgtggcgtgg
     6961 tcggacgtgg cttgcgttcg cggcgcggtg cgtcttcttt ctcaccagca gctgcggcag
     7021 cttcgcgttt tttcgcttgc tgttcagcac gctgtgcctg aacacgcgct ttcgcttctt
     7081 caagctgctt gcgagcatgc tcgacatgtt gctcgtccag ctcaccgcat gggttgccgt
     7141 caagatcgac acgcgttgcg ccgggtttaa caccgtaaag ataacgccag ctcgaagtgt
     7201 agagacgtaa agcggatcgc aattgcgttt tgctcaggtt catttccccg gcaacacgat
     7261 cgaccaaatc ctgaaaaata ccgattttca gcggacgcgc ttcaccttcc gcactgaaac
     7321 agtggggaaa acgttcggcc agaaacgcga ttacttcttt actgctattc aacttaggtt
     7381 gattttccat gaaatttcct gattacaacg gacgtagcca acaagcgcag gcatgaacag
     7441 gcgtcattat aatgacgcta tcagtaaatg ctacgttatc cgttgattat cctgcgacgc
     7501 tcgcaaagaa ttttttgtaa tccgtcgttg caagcacttt ttcaagctgt gccacaagct
     7561 gacgtaagcc ttgctcgtcc tcgtctgtaa agcgaccgaa gacggtacta tcgatgtcga
     7621 gaacaccaat aatctgattt ttcaccacca gtggcagaac aatttcagaa ttactcgccg
     7681 catcacaggc aatatgcccg tcaaacgcgt gaacatcctc gatacgctgc acttgattgc
     7741 gggcaaccgc agtgccgcat acaccgcgcc caacgggtat ccggacacag gcaattttgc
     7801 cctgaaatgg tccgagcacc agtgtatcgt cctcaagcaa ataaaaacct gcccagttta
     7861 cgtcagtgag acgctcatat aacaacgcac tggtgttcgc aagcgttgcc agaaaactgg
     7921 tttctcccgc catcagcgcg ttaaagtcgc gatttaaatc cgcgtaaaat tctgctttgt
     7981 tcattatata atcacttggt tgtcttacct ggaactgcca gcctattaaa ataagcatta
     8041 aatgcgttaa tgctcaagat cattcccatc atgggttaag attaatgtta attcttatta
     8101 catttggcac gtcatggctc ttaacacacc acaaattacg ccgacaaaga agataacagt
     8161 gagggctatc ggcgaggaac tgccgcgtgg tgattaccaa cgttgcccgc aatgtgacat
     8221 gctgtttagc ctgcccgaga taaattctca tcaaagtgcc tattgtccgc gctgtcaggc
     8281 aaaaattcgt gacgggcgcg actggtcgct aacgcgcctg gcggcaatgg ccttcaccat
     8341 gctgttgctt atgccgtttg cctggggcga accgctgttg catatctggc tgttaggcat
     8401 tcgtattgac gccaacgtta tgcaaggcat ctggcaaatg accaaacagg gcgatgcgat
     8461 aacgggggcg atggtctttt tctgcgttat cggtgccccc ctcattctgg tgacctccat
     8521 agcttattta tggtttggta accgattggg aatgaattta cgcccggtac tgctgatgct
     8581 tgagcggctg aaagagtggg tgatgctgga tatctacctg gtcggcattg gcgttgcttc
     8641 tataaaggta caggattatg cccatatcca ggcgggtgtc ggcttgttct cttttgtggc
     8701 gttggtaatt ttaacgacgg tgacgttgtc acatcttaat gttgaagaac tgtgggagcg
     8761 attttatcca cagcgccccg ctacccgtag ggacgagaaa ctccgtgtct gtcttgggtg
     8821 ccattttacc ggctatcctg atcagcgtgg tcgctgcccg cgttgccata tcccgctacg
     8881 cctgcgtcgc cgtcatagtc tgcaaaaatg ctgggcggcg ctgttagcgt caatcgtttt
     8941 gttgttacct gccaacctgt tgcctatctc tatcatttat ctgaatggtg gtcggcagga
     9001 agatacgatt ctttccggaa ttatgtcgct ggcaagtagc aacattgcag ttgcgggaat
     9061 cgtgtttatc gccagtattc tggtaccgtt tactaaagtg atcgtcatgt tcactttact
     9121 gttgagtatt cattttaaat gccagcaagg tttacgcaca cgcattctgt tactgcggat
     9181 ggtgacctgg attggtcgct ggtcgatgct cgacctgttt gtcatatctt taaccatgtc
     9241 gctgattaat cgcgatcaga tcctcgcttt tactatggga ccggctgcgt tttatttcgg
     9301 cgcagcggta attttgacta ttcttgctgt ggaatggctg gacagccgct tactttggga
     9361 tgcacatgag tcaggaaacg cccgcttcga cgactgaagc gcagattaaa aataaacgcc
     9421 gtatctcacc tttctggctg ctgccgttca tcgcgctaat gattgctggt tggctgattt
     9481 gggacagtta tcaggaccgg ggcaatactg tcaccatcga ctttatgtcg gcggatggta
     9541 ttgttccagg ccgtacgcct gttcgttatc agggcgttga agttggaaca gtgcaggata
     9601 tcagcctcag cgacgatctt cgtaagattg aagtcaaggt cagcatcaag tccgatatga
     9661 aagatgcgct gcgcgaagag acacagttct ggttggtgac gccaaaggcc tcgttggcag
     9721 gtgtctccgg gctggacgcc ctcgtcggtg gtaattatat cggcatgatg ccgggtaaag
     9781 gtaaagagca ggatcacttt gtcgcactcg acacccaacc gaaatatcgg ctggacaatg
     9841 gcgatctgat gatccacctg caagcccccg atctcggttc actgagcagt ggttcattgg
     9901 tctatttccg caagatcccg gtgggtaaag tctacgacta tgccatcaat cccaacaagc
     9961 aaggcgtggt gattgatgtc ctgatcgagc ggcgttttac cgatctggtg aaaaaaggta
    10021 gccgtttctg gaacgtttcc ggcgttgatg ccaacgtcag tatcagtggc gcgaaggtga
    10081 aactggaaag tctggcggca ctggttaacg gtgcgattgc cttcgattca cctgaagagt
    10141 cgaaacctgc cgaagcggaa gatacctttg gtctgtatga agacctggcc catagccaac
    10201 gtggcgtaat aataaaactg gaactgccag gcggtgccgg attaaccgcc gactcgacgc
    10261 cgttaatgta tcaggggctg gaagtcggac agctgactaa actggattta aatcctggtg
    10321 gtaaagtcac tggggaaatg accgttgatc ccagcgtcgt caccctgctt cgtgaaaata
    10381 cccgcatcga attacgtaat ccgaaattat cccttagcga tgctaatctc agtgccctgc
    10441 tgaccggcaa aaccttcgaa ctggtgcccg gcgatggcga gccacgtaaa gagtttgttg
    10501 ttgtgccagg cgaaaaagcc ctgctgcaag aaccggatgt tctgacactg accctgacag
    10561 caccagaaag ttacggtatt gatgctggtc agccgctcat tcttcacggc gtgcaggtag
    10621 gccaggttat cgatcgtaaa ctcaccagca aaggcgtcac ctttaccgtc gccatcgagc
    10681 ctcagcatcg cgaactggta aaaggcgata gcaaatttgt cgtcaacagc cgcgtcgacg
    10741 tgaaggtagg gctggatggc gttgagtttc tcggtgccag cgcctcagaa tggatcaatg
    10801 gcggaatacg tattctgccg ggcgataaag gcgagatgaa agccagctat ccactgtatg
    10861 ccaatctgga aaaagcgctg gagaacagcc ttagcgattt acccaccaca accgtgagtt
    10921 tgagtgcaga gacgctgccg gatgtgcagg caggatcggt agtgctgtac cgtaaatttg
    10981 aagttggtga agttattact gtgcgtccgc gagctaacgc gtttgatatc gatctgcata
    11041 ttaagccgga gtatcgcaac cttctgacca gcaacagcgt gttctgggca gaaggtgggg
    11101 cgaaagttca gctgaatggt agtggtctga ctgtacaggc atccccgctc tccagagcat
    11161 taaagggggc tattagcttc gataacctca gcggtgccag cgccagtcag cgtaaaggcg
    11221 acaagcgtat tctgtatgct tccgaaacag cggcccgtgc ggttggcgga caaattacgc
    11281 ttcacgcttt cgatgcggga aaactggcgg tcgggatgcc aattcgctat ctcggtattg
    11341 atatcgggca aatccagacg ctggatctga ttaccgcacg caatgaagta caggcaaagg
    11401 cggtgctcta tccggaatat gtccagacct ttgctcgcgg tggtacgcgc ttctcagtgg
    11461 tcacaccgca aatttcggca gctggcgttg agcatcttga tactatcctc cagccgtata
    11521 tcaacgtcga accaggccgg ggcaatcctc gccgcgactt tgaattacaa gaggccacca
    11581 ttactgattc gcgttacctg gatggcttaa gcattattgt tgaagcgccg gaagccggtt
    11641 cattaggtat cggtacacct gtgctgttcc gtggtctgga agtaggtacg gttaccggaa
    11701 tgacgctggg gacattgtca gatcgcgtga tgattgcgat gcgcatcagt aaacgctatc
    11761 aacacctggt gcgtaacaat tccgtcttct ggttggcatc gggttacagt ctggactttg
    11821 gtctgacggg cggcgtagtg aaaaccggca cctttaacca gtttatccgt ggcggcatcg
    11881 ccttcgccac gcctccgggt acgccactgg caccgaaagc ccaggaaggc aagcacttcc
    11941 tgttgcagga aagtgaaccg aaagagtggc gtgaatgggg aactgcgctt cccaaataat
    12001 gcccactgct ccggcgtgcc tgcgccggag cgtttatgct aaactgcgcg cctgtttttt
    12061 tgccagtggt atatgctcgt ggcccaacac accgtttatt tcccggacgc ctttctgaca
    12121 caaatgcgcg aagcgatgcc ttcgacgctc tcttttgatg attttcttgc cgcctgtcag
    12181 cgcccgttgc gccgcagcat tcgcgttaat acgctgaaaa cctccgttgc tgatttcctg
    12241 caattaactg ctccttatgg ctggacgctt acgccaattc cgtggtgtga agaaggtttc
    12301 tggattgaac gcgacagtga agatgcattg ccgttgggta gtaccgccga gcatttaagc
    12361 ggcctgtttt atattcagga agccagttca atgttgcctg ttgccgcctt gtttgctgac
    12421 ggtaatgcac cacagcgggt gatggatgtc gctgctgcac caggttccaa aacgacgcaa
    12481 attgctgcgc ggatgaataa cgaaggggca atccttgcca atgagttttc cgccagtcgg
    12541 gtaaaagtgt tacatgccaa tatcagccgc tgtggcatca gtaatgttgc gctcacgcat
    12601 tttgatggcc gcgtgtttgg tgcggcagtg ccagaaatgt tcgatgccat tttgctggac
    12661 gctccctgct ccggcgaagg cgtggtgcgt aaagatcccg atgcgctaaa aaactggtca
    12721 ccagaaagca atcaggaaat cgcagcgacc caacgggagc tgatcgacag cgcctttcat
    12781 gcattacgtc ctggcggtac gctggtttac tcgacctgta ccttaaaccg ggaagaaaac
    12841 gaagccgttt gcctgtggct gaaagagact taccacgacg cagtagagtt tttaccgctt
    12901 ggcgatctct tccctggtgc aaataaggcg ctgaccgaag acggcttttt gcatgttttc
    12961 ccacaaattt acgactgcga aggcttcttc gttgctcgtc tgcgtaaaac tcaggcgatc
    13021 cccgtcttac ccgccccaaa atacaaagtg ggtaatttcc cgtttagccc ggtgaaagat
    13081 cgcgaagccg gtcaaattcg tcaggcggct gcaagtgttg gcttaaactg ggatgaaaac
    13141 ctgcgactct ggcagcgtga caaagaactg tggttgttcc cggtaggcat tgaagccctg
    13201 atcggtaaag tccgattttc tcgcttgggg attaaacttg ccgaaacgca caacaaaggt
    13261 tatcgctggc agcatgaagc cgttattgcc cttgcctccc ccgacaatgt gaacgctttt
    13321 gaactgacac cgcaggaagc ggaagagtgg tatcgcgggc gcgatgttta cccgcaagcc
    13381 gcgccagtgg cggatgatgt attggttact ttccagcatc agccgattgg tttagccaaa
    13441 cggattggtt cacgactgaa aaacagctac ccgcgtgaac tggtgcgcga cgggaaactt
    13501 tttaccggta acgcctgaca gcgcacaaaa aaagcgcact ttttgactgg cacattcgac
    13561 tgcctcaact aggctgaaaa atggtgcgat cggactggtc gtaccacaac cggcagctaa
    13621 atggagagca cgaagatgaa aaccagtgtg cgcataggcg cttttgaaat cgacgacggc
    13681 gaattacacg gcgaatcgcc gggtgatcga acgttaacca ttccttgtaa atcagacccc
    13741 gatttatgta tgcaactgga tgcctgggat gctgaaacca gtatccctgc cctgctaaat
    13801 ggcgaacact ctgtccttta ccgtacccgt tacgatcaac aatctgatgc ctggattatg
    13861 cgtcttgcct gatccaaaaa gaacccgtcg gcatggcggg ttatttgtcc gggttattcc
    13921 cccgttgtaa aatctctcct aaacttaacg gtacggcacc acacttcggg gatgaaatgt
    13981 tcgcgctggt actttttgtt tgctacctgg atggcggttg tgaagatatt gttgtggatg
    14041 tctacaacac ggaacagcag tgtctttatt ctatgagcga tcaacggatc cgccatggcg
    14101 gttgttttcc gattgaggat tttatagatg gtttctggcg acctgcacag gagtacggtg
    14161 atttttaatt attgcaattg cacaagagtc agttctcccc caaagacagc accggtatca
    14221 atataatgca ggttgccaat atccacgcga tggcgcaaag gtgtatgacc aaaccagaaa
    14281 tgatcagcac ctgcaattcc ctgccctttt tggcgttcac ctaatcgcga gcggctccac
    14341 aagacctgat gcaaatcgac gtccttttgc cattcataga catcatctgg ataatcggca
    14401 tgagcaataa catgtttgcc ggtgcgactg tgtaattcaa gaataaaggg caaatgctga
    14461 catttttcca gcgccgtttt cgcttgtttc tgttgattat ctgccagcgc aataaaccag
    14521 tcgccgccat ttatcaacca taaagacatc tgccgggatg ccagcgcatc catcgccatc
    14581 tgttcatgat tgcctcttac cgcacgaacc caatgttgtt ccagtaactg caggcaacgt
    14641 aaactttgcg gcccacgatc gataacgtct cccactgaga taagtaaatc tcgccacgga
    14701 tcaaaacgac aatgccataa tttgcggcgc aactgctcaa gacaaccgtg tatatcgcct
    14761 gaaagccaga tatgtcgcca ttgatgaccc gcaattctct gataaacggg cgcaggctgt
    14821 ttcatcaata ttttcctccc gcgctaaaga tcacataatc ttaacaagaa tgttaaaaaa
    14881 cgctggactc agacagtaga gtgtgtgtta tggttgacta taaagtcagc gaagggaatg
    14941 cttctggctt ttaacagata aaaagagacc gaacacgatt cctgtattcg gtccagggaa
    15001 atggctcttg ggagagagcc gtgcgctaaa agttggcatt aatgcaggct tagttgcctt
    15061 gctctttaag aatagatgac gacgccaggt tttccagttt gcgtgcaaaa tggtcaataa
    15121 aaagcgtggt ggtcatcagc ttaaatgtta aaaaccgccc gttctggtga aagaactgag
    15181 gcggtttttt attggaaatc aaaaggctat tttaggtaat taacagagtt tttcagctcg
    15241 ctctataaac ggtgccagac tcattttttc gccgggattg ttaggatcat caatctgaat
    15301 caccgaaatg ggttgggctt tagtcttccc actggcaact tccttttgtg cgatatcgtt
    15361 taaaggatac tgcacgagag tacttgggtt aatgacatac aaagcattac ctggtcggca
    15421 agtcagcatc acctcttcgc gattaaacgc ccatttttct ttacccactt caaaacggct
    15481 gacggtaatc acctgcggtg ccgccagcgc agctgcagaa ctggtgagta acagaaacgc
    15541 cagaatactg tttttcatca ttttcgtaat ccatcaaaaa ggttcgagag tcgcaagcag
    15601 gctgatgatc accagcacta ccgcacctat cgcccattcg agtttagtca tccagacaaa
    15661 ccagggagcc gcacgatctt catcctgcct catgcgcggt acaagaacat aacgattagc
    15721 cagcgcgatg accaccataa tcatcaccag gatggctttg agcaataaga gttggcccca
    15781 gtaagtcgta agcgtggggg gaaatccggt aatcaacaat gcattaagca cgccgcttgc
    15841 cagtacgccg atcaccgcaa aatgcccgca ccaggaaaaa cgcatcagcg cctgaatagc
    15901 ctggtgtcgc caacggcctt tgatgagttg catacaccag agcaccggca gtaatccccc
    15961 aaaccacgcc gccgcacaga tcaggtgaat tgcgtgatta gtctgatgga ttttcgccgt
    16021 taccccttca ttcaacgtcg catgcccgac tcccgccagc agaataaatt gcgcggtagt
    16081 gagcataaac agcaaacgtg gcatattccg cggttgcata agggcgacga tcaacgttac
    16141 gagggcgaga acaatttgcc ataaccagac gccaccaaac tgcgtttgca aaacagccaa
    16201 ccaaatgttt ggcgaaaata catctgacca tcccgctccc atcaacccgc cctgaacggc
    16261 gagcattgca gtggcactga tcaaactcca gacggcagcg tgctgttgta aacgcagaaa
    16321 acgcttcgtc agcaagcgac gaatcgttaa gggggccagc caggcaccgt acattgcgaa
    16381 gccaaaaacc agcatcagcg aggtgaaatg gataaatcgc agcgcgatcc aggtaaacgc
    16441 cagcatgatt tatttcacac taaaggtgta atgtcctttc gttttatgcc catccaccga
    16501 aacaacatgc cagtcaacgg tgtaggttcc gggtttcagc gaatcggcca gtggaacaat
    16561 cagttgcttt tggtcctgct cattccgctt cgccggtaat gttttaatat tttcgttttt
    16621 tggccccgtg atttttgcac cactgaatcc tgtttcaacg ccttccgaga agtttaaagt
    16681 gattgcctgc ggagctgctg tcacttgtgc atttgccgca ggatactgat gcgttaaatg
    16741 cgcatgtgcc cagacggaag gtgtcaccaa cgaagtggtc aggattgcca gcgcgtagcg
    16801 aagggagcgt gcagttgaag ccatattatc tattcctttt tgtaataact tttttacaga
    16861 gcataacctt gtctaatgtc tgagtcgagg atcatcaatt ccggcttgcc atcaaggctc
    16921 actcttagta acttttgccc gcgaatgatg aggagattaa gaatgctgaa gaatctggct
    16981 aaactggatc aaacagaaat ggataaagtg aatgtcgatt tggcggcggc aggggtggca
    17041 tttaaagaac gctacaatat gccggtgatc gctgaagcgg ttgaacgtga acagcctgaa
    17101 catttgcgca gctggtttcg cgagcggctt atcgcccacc gtttggcttc ggtcaatctg
    17161 tcacgtttac cttacgagcc caaacttaaa taaaacttat acatagttac actttcttac
    17221 ataacgcctg ctaaattatg agtattttct aaaccgcacc cataatttgc agtcattttg
    17281 aaaaggaagt cattatgtcg ttctggaaag ttgcagcagc gcaatatgag ccccgtaaaa
    17341 cctcactcac cgaacaggta gctcatcatc tggagtttgt gagggccgcc gccaggcaac
    17401 aatgtcagct gttagttttt ccatcgcttt ctttactggg gtgtgattat tcgcgacgtg
    17461 cccttcctgc cccacccgat ctctcgctat tagacccgct ttgttatgcc gcaacgacct
    17521 ggcgaatgac catcattgcc ggccttcccg ttgaatataa cgatcgcttt attcgtggaa
    17581 ttgcggtatt cgcaccgtgg cgaaaaacgc cagggatcta ccatcagagt tatggtgcat
    17641 gtctgggccg tcgttccaga accatcacgg tggtcgatga acaaccgcaa ggtatggata
    17701 tggacccgac ctgttcactg tttacgacag ggcaatgtct gggagaaccc gacctgctgg
    17761 cgtcggcccg tcgcttacaa tttttttcac atcagtactc gattgcagtg ctgatggcaa
    17821 atgcccgtgg taacagtgca ctatgggatg aacatggtcg tctcatcgtt cgcgccgatc
    17881 gtggttcatt attgttagtc ggtcagcgtt catcacaggg ttggcaaggc gatatcattc
    17941 cattacgcta ggcttttcgg cctggagcat gccatgttgc gcattatcga tacagaaacc
    18001 tgcggtttgc agggagggat cgtagagatt gcctctgttg atgtcattga cggaaaaatc
    18061 gtcaatccca tgagccacct ggtgcgtccc gatcgtccta ttagtccaca ggcgatggcg
    18121 attcatcgca tcaccgaagc catggtcgcc gataaaccgt ggattgaaga tgtgatcccg
    18181 cactattacg gtagtgaatg gtatgtcgcg cataacgcca gctttgaccg ccgcgtactg
    18241 cctgaaatgc ccggtgagtg gatttgcact atgaaactgg cccgtcgttt gtggccaggg
    18301 atcaagtaca gcaatatggc gctatataaa acacggaagc tcaatgtaca gacgccgccg
    18361 ggcctgcatc atcaccgcgc gttgtatgac tgttatatca ccgccgcgtt gcttatcgat
    18421 attatgaaca cctctggctg gacggcagaa cagatggccg atatcaccgg acgtccatcg
    18481 ttgatgacga ccttcacctt tggcaaatac cgtggcaaag cggtttccga cgttgccgaa
    18541 cgcgatccgg gctatctgcg ctggttgttt aataacctcg acagcatgag cccggagctg
    18601 cgtttaacac tgaaacatta tctggaaaat acttaatccg ctgactgagc gggtaatgtt
    18661 ccctgcgcca gcgcgaccag aaaagcatat tccatcgcta cgccttcgta cgatttgaag
    18721 cgaccagatt taccgccatg acctgagtcc atgtcggtac agagcaataa aagatggtca
    18781 tcggttttca gctcgcgcaa tttagcgacc cattttgccg gttcccaata ttgcacctga
    18841 gaatcgtgta aaccggtcgt taccagtaaa tgcggataag cctgtgcggt gacgttgtca
    18901 tacgggctgt agcttttcat atactcgtaa tattgcggat cctgcggatt cccccactct
    18961 tcaaattcgc cggtggtaag aggaattgat tcatccagca ttgttgttac aacatcaaca
    19021 aacggtacct gggcgataac gccgtggaac aattccgggc gttgattaat cgcaacgccc
    19081 atcaacatac cccccgcact cccgcccatc gcataacaga gcgaaggaga gccatagccc
    19141 agttttaaca atgcatcgca ggcatcaaga taatcattaa acgtattttt cttcttcaga
    19201 aatttaccgt cctcgtacca ttgttgcccc agctcaccgc cgccacgaac atggacaatg
    19261 gcgtagacaa atccacgatc caacaaactc aagcggctaa aactgaaatc ggcatcaata
    19321 cttgcgccgt aagaaccgta gccatacacc agcagcgggt tgtgtccttt gcgaaaatgt
    19381 ttgcgatgat agaccagcga aaccggaact tcgacgccat cacgggcgac tatccacagg
    19441 tgttcactgc ggtaattcgc cgcatcaaaa ccaggaactt ccgtttgttt taatacacga
    19501 cgctcaccgg tatccatatc cagttcaaac aaagtatctg gtgtggtcat ggacgaataa
    19561 ccataacgca atcgcgcggt ttcaggttct ggattgtagg caatccaggt cacataggcc
    19621 ggatcatcaa aggcaatgcc aatgacttcc cgggtcttgc ggttaatttg ccgcaaactg
    19681 gttaacccgc gctgacgctc ttcaaccacc agccagtcgg taaacagcgt atacccttcc
    19741 agcctaatgt tatcacgtgg cggaattaac tcttcccact gttgctcatc acgcatacgg
    19801 gtacggtata agccaaagtt tttaccgtgg cggttggaac gcagataaaa ccgatgctgg
    19861 tagtgatcaa ggctgtattc gtgatctttg cggcgaggca gaaaaacaaa cggctcggca
    19921 tcggccattt ccgcgtccag caggcgaact tcactggtgg tggcgctggc cagatgaatg
    19981 accacataat gcttcgaggt cgttttatgc aggctgacgt aataggtatc gtctttttct
    20041 tcgtaaatca gtttatcttg cgatgctggc gtaccgatgg cgtgacgcca gacctgataa
    20101 ggcagcagcg tcactggatg cttgcgaaca tagtagaaag tccaggagtc atttgcccag
    20161 acaaagctgg gttcaacgtt atccagcagt tccgggtacc agttacctgt ttccagatta
    20221 cgaaaacgaa tgccgtactg ccgtcgggaa agaaaatctt ctgccagtgc cataatggtg
    20281 ttatctggcg taatcgccat tccgcccatc gaataaaact cactatgagc cgcgcgcttg
    20341 ttggcatcga gcaatgtttc ccactcgtcc cactcttcac tgaacgccga ttgacgctgg
    20401 tagatagcat attcacagcc tggttcataa atatgccgat agcggtagcc atttttgatg
    20461 tagggcgcag aaacttctcg ttgcggaatg cggtcgatga tttcctttaa gatgcgatcc
    20521 tgcaaggctt gttgcgaggc catcacccga tgaccgtaac tattttcttg ttgcagatag
    20581 tccaggactt ccggctgaga acgcgtatcg tcccgcagcc agtagtaatt atcgatgcgc
    20641 gtatcgccat gaagcgtcat ggcgtgggga atgcgggcgg cttttggtag catgttattg
    20701 ttctttctgg ttgaaacatc ttataagggt ggcaaaactc accgtggatg caagcgaaac
    20761 agggtagtca ttgcttagat gatgacaggt aatggcgcgg atatcgactg ttatgcagac
    20821 agagaaaatc agcctgttca aatggctgtg cggttctgga taacccgaaa taatcagctt
    20881 caggctatcc agagagcgga attactccgc caaagtacgt ttttgctgtt cgagatcgcg
    20941 ttcaatgcct tcacgaacat cctgggggat tttcagcgca tcacccagtg cattcaggta
    21001 actgcgttcc ataaaatggt caatatcaat agccgcgcag ctcaggaaat agatttccag
    21061 cgcctcttct tcattgcgga ccccggtagc caggcgttgt ggatccagcg gttgttcgat
    21121 tgcctgctca atgagtacac gccccttctc ttccacaccg gcttctcgca attgctggtc
    21181 gattgccgca cgttctttgg catcaatatg gccatcactt ttagcggcaa agaccagcgc
    21241 aaggatcaaa cgttccgtac gctcatccag cggcgtactt tgcgcgccaa actgcggttc
    21301 gtcctgatgc gccgcgcgaa ttttatcttt gtatttattc cacagcaccg tacccgctac
    21361 cgctccgccg ccaaccagta acgcgtttgt gccatatttc gtaagaagtt tacgtgctga
    21421 tttatttgcg accagcagcc cagccagtcc gtctaatgcg ccaggcacta aaagtttgcc
    21481 caatccctga tccgcagacg aggaggtaga agaacggctt tgcccaagca gggattgcag
    21541 ttgatttaac cagttagcca tatttgctcc tcaataacca tttttagtgc ctgtagcata
    21601 gcggatgagg atgtcagaaa atgtatatcg agacaaaaga tgcgcaaata agcggggcgg
    21661 taggccgtta ttcaaagaaa ttcgcgccag gtaaggtgtt cactgtatat aaggtgataa
    21721 gggtatttta ggtgaaagta gtttgacatt ttgagtggaa ctgtttgatg ctgattgccc
    21781 ggttcgacgc tactgcgtct tctcaggcct acgattgtgt tgcgttgtag ggcggacaag
    21841 gcacccgcgc cgcatccgcc aacggtcaat cgaattatca gaagccatta acgccgctga
    21901 tattccgcca ttcccgcttt gcagtccacg ctgacctggt aatgaatatc ggcactttta
    21961 ccacgcacgg ttagcggtac tgaccattta tcatctttcc cctgaatgtc ctgcaaactg
    22021 acccatgcca cgggatcggc ctgaccgaca attttttgat catcagccca gcgcgccacg
    22081 cgattttgtt ggtaatcacg ttttacgctc gcggcaattc cggcagcatc cagaccttca
    22141 cactttggga aagtgaccga cttgctggtt tcattattgg cagcgaaaac tgatgcgcag
    22201 gcagaaacca acaacagccc taaaaacgcc cctctttttt tcatgttttt ctccatagca
    22261 caatgattca ggagaaagca tggtacaaat tgtcaggagc gcaagttgct tcaggccgcg
    22321 tgtgaggcat cttcctcttc ggattcagca ccgacgacct gtggggacgg taatttgccg
    22381 gttttcagaa tggtgctcag cacatctttt tgttctgcca gccatagcga aagggcttca
    22441 cgttgctcat cttccatttg cactggagaa tttgtcagcc aggtgtcaag cagatcggcc
    22501 gtatcaagca tcttgtcata agcatcggct tcctttttgc tggtaaacga cattttctct
    22561 tcgccctcac gaatgactac gtatttaact tcaaccgcca tttgcagcct ctcataataa
    22621 ctgtaatttt atacagtata tttcttttca gttgggaaat caacatcagc aataaagaca
    22681 cacgcaaacg ttttcgttta tactgcgcgc ggaattaatc aggggatatt cgttatgacg
    22741 ttattaggca ctgcgctgcg tccggcagca actcgcgtga tgttattagg ctccggtgaa
    22801 ctgggtaaag aagtggcaat cgagtgtcag cgtctcggcg tagaggtgat tgccgtcgat
    22861 cgctatgccg acgcaccagc catgcatgtc gcgcatcgct cccatgtcat taatatgctt
    22921 gatggtgatg cattacgccg tgtggttgaa ctggaaaaac cacattatat cgtgccggag
    22981 atcgaagcta ttgccaccga tatgctgatc caacttgaag aggaaggact gaatgttgtc
    23041 ccctgcgctc gcgcaacgaa attaacgatg aatcgcgagg gtatccgtcg cctggcggca
    23101 gaagagctgc agctgcccac ttccacttat cgttttgccg atagcgaaaa ccttttccgc
    23161 gaagcggttg ccgccattgg ctacccctgt attgttaaac cggtgatgag ttcttccggt
    23221 aaagggcaga cgtttattcg ttctgcagag caacttgctc atgcatggga gtacgctcag
    23281 caaggtggtc gcgccggagc gggccgcgta attgttgaag gcgtcgttaa gtttgacttc
    23341 gaaattaccc tgctaaccgt cagcgcggtg gatggcgtcc atttctgtgc accggtaggt
    23401 catcgccagg aagatggcga ctatcgtgaa tcctggcaac cacagcaaat gagcccgctt
    23461 gcccttgaac gtgcgcagga gattgcccgt aaagtggtgc tggcactggg cggttatggg
    23521 ctgtttggtg tcgagctgtt tgtctgtggt gatgaggtga ttttcagtga agtctcccct
    23581 cgcccgcatg acaccgggat ggtgacgcta atttctcagg atctctcgga gtttgccctg
    23641 catgtacgtg ccttcctcgg acttccggtt ggcgggatcc gccagtatgg tcctgctgct
    23701 tctgccgtta tcctgccgca actgaccagt cagaatgtca cgtttgataa tgtgcagaat
    23761 gccgtaggcg cagatttgca gattcgttta tttggtaagc cggaaattga tggcagccgt
    23821 cgtctggggg tagcactggc tactgcagag agtgttgttg acgccattga acgcgcgaag
    23881 cacgccgccg gacaggtaaa agtacagggt taaacccggc aaaaaaacgc tacaaaaatg
    23941 cccgatcctc gatcgggcat tttgactttt acagcttagc gccttctaca gcttcacgcg
    24001 ccagcttagt aatgcggtcg taatcgcccg cttccagcgc atctgccgga accagccagg
    24061 aaccaccgat gcacagcacg cttttcagcg ccaggtagtc acggtagtta gccggagaaa
    24121 taccacctgt cgggcagaaa cggacctggg agaacggacc cgcgatcgcc tgcagggctt
    24181 tcacaccgcc gttagcttca gccgggaaga atttgaactc tttcaaacca tagtccatac
    24241 ccagcatcag ttcggaaaca gtgctgatcc ccggaatcag aggaatagtc ccttcggtag
    24301 cagctttcag cagcggctcg gtcagacccg ggctaattgc aaactgtgca cctgcttcag
    24361 tgacttctgc cagctgctgt ggattcagca ccgtaccggc acccacaatc gcttcaggca
    24421 cttctttggc gatagcacgg atagcgtcaa ctgcgcactc ggtacgcaga gtcacttcca
    24481 gaacgcgcac cccaccagca accaacgctt ttgccatcgg caccgcgtgt tccagttttt
    24541 ttaccacgat aaccggtaca accgggccgg tggtcaggat tgattctgca cttgttttcc
    24601 agtttttcat cagagttttc tctcgcctga ttacaaattt gtcgtcttaa aaagtgatac
    24661 aggttgcgcc ctgttcggca ccggacagtt tttcacgcaa ggcgctgaat aattcacgtc
    24721 ctgttcccac gcgtgacgcg ctcaggtcag gaatgtgcgg ttcgcgagca gccagttccg
    24781 cttcgtctac cagcagcgtc agttcgcctg tctgtccatt cacacgaatg atgtccccgt
    24841 cgcgcacttt tgccagcagc ccgccatcgt aggcctctgg tgttacgtgg atagctgacg
    24901 gcactttacc tgaagcgccg gagagtcgtc catcggtaac taacgcaatt ttgaaacacc
    24961 ggtccaataa tacaccaagt ggcggcatga gtttatgtaa ttctggcatt ccgttcgctt
    25021 ttggcccctg atgacggaca acaacgacac aatcgcggtc cagtaaaccc gcttcaaagg
    25081 ccggcataac gtcatgctgg ctttcaaaaa caaccgctgg cgcttcaatc acctggttct
    25141 ctaccggcac ggcagaggtt ttcataaccg ctcgccccag gttaccgctt aacacttttg
    25201 tcccaccatg atgagagaaa ggttgttcga aggacgcgat cacattgttg tcgagtgatt
    25261 tttctgcccc ttcccgccag tccagttcac cattattcag ccatggttca agggtataac
    25321 gagacagacc aaagcctgcc accgtattga catcttcatg cagcaggcct gctttgagca
    25381 gttcacgtac cagaaccggt acgccacctg ccgcctggaa gtggttaata tcggccggac
    25441 cgttcgggta gagacgtgcc atcagcggta caacatcaga aaggtcagag aagtcatccc
    25501 agttaatctg aataccggct gcgcgcgcca tcgccaccag gtgcatggtg tggttagtgg
    25561 aaccaccggt cgccagcagt gcaacgatac cgttcaccac cactttctca tcgatcatct
    25621 taccgatcgg catccattca ttaccattac cggtcatgcg cgtaacctgg cgcgcagctg
    25681 cagcggtcaa agcatcgcgc agcggagaat ccggatgaac aaaagaagag cctggcaact
    25741 gcatccccat aaactccacc accatctggt tggtgttggc agtaccgtag aaagtacatg
    25801 ttcccggcgc atgataagac gcggcttctg actccagtag tgccatgcgg tccactttac
    25861 cttcggcata aagctggcga atacgcactt tttctttatt tggcaaaccg cttgccatcg
    25921 gtccagacgg cacaaacacc gcaggcaaat gaccaaacga cagggctgcc atggtcagac
    25981 ctgggacaat cttgtcgcac acaccgagaa acagagcacc atcaaacatg ttatgggaca
    26041 gccccaccgc cgcagacatc gcaatcactt cgcggcttaa cagcgacaat tccattccat
    26101 cctgcccctg ggtgacacca tcacacatcg ccggaacacc gcccgcaacc tgaccaaccg
    26161 cattcgcttc atgcagggct ttacgaatga tttccggata gtgttcataa ggctggtgag
    26221 cggagagcat gtcgttatag gaggtgatga tggcgatatt gttacgcaac atgcttttca
    26281 aagaggcttt gtcttctggc tggcaggcag cgaaaccgtg tgccaggtta ccgcatgcca
    26341 actgcgaacg atgaacggtc gaagttttcg cttgttctat ccgggcgagg taagcagagc
    26401 gagtctcgcg cgaacgttca atgattcgat ttgttacgcg taacaattgt ggattcataa
    26461 aggctcctga aattgagttg tcagagcagg atgattcaca acacgtttca ttcagaggat
    26521 tcatgactga aacgcctgta accggagctc atagggcaaa aacgtttcag tcagtgtaat
    26581 aaaaaaagcc tcgtgggtga atccgcacga ggcctgaaag tgtaaaaatt gttctacaat
    26641 ctgtgcaaga tcatgttacc ggtaaaataa ccataaagga taagcgcaga tattactcaa
    26701 actcattcca ggaacgaccg tcacgggtaa tcatcgccac cgaggcaacg gggccccagg
    26761 ttccggcctg atacggtttc ggcgcatcat tgtccatcgc ccacgcctca gtaatggagt
    26821 ctacccattt ccaggcttct tccacttcat cacgacgtac aaacagtgcc tgaataccac
    26881 gcatggtttc cagcagcaga cgttcatagg catctgccag atgcgtttga ttaaaagttt
    26941 ctgaatagct cagatccagc ttggtgattt gcaggttatg tttgtggtca aggccaggaa
    27001 ctttattcag cacctggata tccacgcctt catcaggttg cagacggata gtcagtttat
    27061 tctgcggcag atcctgccac gactctttaa acagattcag ttcaggtgtt ttgaaataga
    27121 ccacgacttc agaacatttg gtcggcagac gtttaccagt acgcaggtag aatggcacac
    27181 cggcccagcg ccagttatca atgtcgacgc ggatcgccac gaaggtttct gtattgctgc
    27241 tcttgttcgc gccctcttct tccagatagc ccggcacttt tttgccctga gcgaagcccg
    27301 cagtatattg cccgcgtacg gttttttcgc gcacgttgga gcggtcgatg cggcgcagag
    27361 acttcagtac tttcactttt tcatcgcgga tgctgtctgc gctcaggtca gacggcggag
    27421 acatcgcaat catgcaaaga atttgcagca ggtggttctg gatcatgtcg cgcatctgac
    27481 cggctttatc aaaatagccc cagcgccctt cgatccccac ttcttctgcc acggtaatct
    27541 caacatgatc aatggtgcga ttgtcccagt tgttcacaaa cagagagtta gcaaaacgca
    27601 gcgccaacag gttcagcact gtttctttac caagatagtg gtcgatacgg taaacctggc
    27661 actcctcgaa gtattcgcca acctgatcgt tgatttcctg cgaggtcgcc agcgacgtcc
    27721 ccagcggttt ttccatgact acgcgtgccg gtttagcatt cagttttgcc tcaccaagcc
    27781 ctttgcaaat tgcgccaaaa gtgctgggcg gcatggcaaa gtagttaatg gtgatacgat
    27841 ttttttgatc cagcatcgcg ccgagacggc tgaatgcagc agtgtcattg acatcaagat
    27901 tacaaaaatc cagacgtgca ctcagggtgt cccataaacc ttcatcaatg gtttctttca
    27961 tgaaagtttc gagcgcctcg cggacaactt tggtataagc cgctttatcc cagtcagcac
    28021 gccctacgcc gataatccgg gtgtccgggt tgagctgacc ggctttttcc agttgataca
    28081 gggaaggcag caatttacga cgcgcaaggt cgcctttcgc gccgaaaatg accaggtcac
    28141 aggcctgggc tgtttgcgtt accgccatgt cattctcctt aagttaacta acccggtact
    28201 taagccaggg tatacttgta attttcttac ggtgcactgt actgctttta cgagcttgcg
    28261 aaaactgtaa acacttatcc acccgtgcga ttacggtaaa agcgcgcaaa gtgtggcaaa
    28321 actgataaaa aaatcatcgt tttccattgg gtaaaaatct gacactgatc atgttatgaa
    28381 aaaaaataac aactttttta tctgtttttg tcattaacac agcacacagg cgtaatatct
    28441 gacaaaactg catttcgatt tctttcagag cggaaatcgt cattacccgt gagtctcttt
    28501 acatcatgaa tatgctggaa aaaatccagt ctcagctgga acatttgagc aaatctgagc
    28561 gcaaagttgc cgaggtcatt ctggcttcgc ccgataacgc gatccattcg agtattgctg
    28621 ctctggcgct ggaagccaat gttagcgaac cgacggtgaa tcgtttctgt cgcagcatgg
    28681 acacacgcgg ttttcctgat tttaaacttc atctggcaca aagtctggcg aatggcactc
    28741 cctatgttaa tcgcaacgtc aatgaagatg acagcgttga atcatacacg gggaaaatat
    28801 ttgagtccgc aatggcaacg ctcgatcatg tccgtcattc actggataaa tctgccatca
    28861 accgcgccgt cgacttgctc actcaggcaa aaaaaatcgc ctttttcgga ttaggttctt
    28921 cagccgccgt tgcccacgat gcgatgaata agttctttcg ttttaatgtt ccggtggtgt
    28981 actccgatga tatcgtgctg caacgcatga gttgtatgaa ttgtagcgac ggagacgtgg
    29041 tggtgctgat ttctcatact ggaagaacaa aaaatctggt cgagctggcg cagctggcac
    29101 gcgaaaacga cgctatggta attgccctca cctctgcggg taccccgctc gcccgggaag
    29161 caacgctggc aattaccctc gacgtaccgg aagatactga catttatatg cccatggttt
    29221 ctcgacttgc acagctgacc gtgatagatg tgctggcgac aggatttact ttgcggcgcg
    29281 gtacaaaatt cagagataac ttgaagcggg tcaaggaagc gctgaaggaa tcgcgttttg
    29341 ataagcagtt acttaattta agtgacgatc gctaaaaacg attgtcactg tcctaatctt
    29401 atacgacatc cgaatgagat taatttatcg ccatcgcggc gttatttcat tcggatttca
    29461 tgttcaagca acacctggtt gtttcagtca acggagtatt acatgtccag aaggcttcgc
    29521 agaactaaaa tcgttaccac gttaggcccg gcaacagatc gcgataataa ccttgaaaaa
    29581 gttatcgcgg cgggtgccaa cgttgtacgt atgaattttt ctcacggctc gcctgaagat
    29641 cacaaaatgc gcgcggataa agttcgtgag attgccgcaa aactggggcg tcatgtggct
    29701 attctgggtg acctccaggg gcccaaaatc cgtgtatcca cctttaaaga aggcaaagtt
    29761 ttcctcaata ttggggataa attcctgctc gacgccaacc tgggtaaagg tgaaggcgac
    29821 aaagaaaaag tcggtatcga ctacaaaggc ctgcctgctg acgttgtgcc tggtgacatc
    29881 ctgctgctgg acgatggtcg cgtccagtta aaagtactgg aagtccaggg catgaaagtg
    29941 ttcaccgaag tgaccgtcgg tggtcccctc tccaacaata aaggtatcaa caaacttggc
    30001 ggcggtttgt cagctgaagc gctgaccgaa aaagacaaag cagacattaa gactgcggcg
    30061 ttgattggcg tagattacct ggctgtctcc ttcccacgct gcggcgaaga tctgaactat
    30121 gcccgtcgtc tggcacgcga tgcaggatgt gatgcgaaaa ttgttgccaa ggttgaacgt
    30181 gcggaagccg tttgcagcca ggaggcaatg gatgacatca tcctcgcctc tgacgtggta
    30241 atggttgctc gcggcgacct cggtgtggaa attggcgatc cggaactggt cggcattcag
    30301 aaagcgttga tccgtcgtgc gcgtcagctt aaccgcgcag taatcactgc gacccagatg
    30361 atggagtcaa tgattactaa cccgatgccg acgcgtgcag aagtcatgga cgtagcaaac
    30421 gccgttctgg atggtactga cgctgtgatg ctgtcggcag aaactgctgc tggtcaatac
    30481 ccgtcagaaa ctgttgcagc catggcgcgc gtttgcctgg gggcggaaaa aatcccgagc
    30541 atcaacgttt ctaaacaccg tctggatgtt cagttcgaca atgtggaaga agccattgcc
    30601 atgtcagcaa tgtacgcggc taaccacctg aaaggcgtta cggcgatcat caccatgacc
    30661 gaatcgggtc gtaccgcgct gatgacctcc cgtatcagct ctggtctgcc aattttcgcc
    30721 atgtcacgcc atgaacgtac gctgaacctg acagctctct atcgcggcgt tacgccggtg
    30781 cactttgata gcgctaatga cggtgtagca gctgccagcg aagcggttaa tctgctgcgc
    30841 gataaaggtt acttgatgtc tggtgacctg gtgattgtca cccagggcga cgtgatgagt
    30901 accgtgggtt ctactaatac cacgcgtatt ttaacggtag agtaagtacg ttgccggatg
    30961 cggcgaaaac gcctcatccg gcctacagtt caatgataat tcaacagatt tcgaatcttc
    31021 tgaagcaaac ttgaacttat catcagtcga aggcctctcc tcgcgagagg cattttttat
    31081 ttgatgggat aaagatcttt gcgcttatac ggttggattt cgcccggttt gcgagttttc
    31141 agcaatttta atatccaggt gtattgttct ggtcgcggac caacaaaaat ctcgacttct
    31201 tcattcatcc gccgcgcaat cgtatgatca tccgcctcta acagatcatc catcggtggg
    31261 cgcacctgaa tcgtcagacg atgcgtcttg ccatcataaa tcggaaacag cggtacaacg
    31321 cgcgcacggc acactttcat caaacgacca atcgcgggca acgtcgcttt ataggtggca
    31381 aagaaatcaa caaattcgct gtgttctggg ccatgatcct gatcgggtaa ataatatccc
    31441 cagtaaccct gacgtaccga ctggatgaat ggtttaatac catcatttct cgcatgcaga
    31501 cgaccaccaa agcgacggcg caccgtgttc cagacataat caaaaaccgg gttgccctga
    31561 ttatggaaca tcgctgccat tttctgccct tgcgaggcca tcagcatggc aggaatatcg
    31621 acggcccaac cgtgcggcac cagaaaaatc actttctcgt tattacgccg catctcttcg
    31681 atgatctcca gcccttgcca gtcaacgcgc ggctgaattt tctccggccc gcgtattgcc
    31741 aactcagcca tcattaccat cgcttgcggc gcggtggcaa acatctcatc tacaatcgct
    31801 tcgcgttcag cttcactacg ttctggaaag cagagcgaca gattgattaa cgcacgacgg
    31861 cgtgagcttt ttcccagtcg tccggcaaaa cgtcccagcc gtgccagaat gggatcacgg
    31921 aactttggcg gcgttaaagc gatacccgcc atcgctgcta cgcccagcca tgctccccag
    31981 tagcgcgggt ggcgaaagga tttatcaaac tcaggaatgt attcgctatt attttttttc
    32041 gtttccatgc ttttccagtt tcggataagg caaaaatcaa tctggtgata gtgtagcggc
    32101 gcaacttgcc ccgcaccaaa taaaaaagcc ggtactgact gcgtaccggc tgcgaatgga
    32161 tgttaattaa tcaaaccgta gctgcggcac aatctctttg gcctgtgcca ggaattcgcg
    32221 acgatcggag ccggtcagcc cttcggtacg cggcagtttt gccgtcagcg ggtttacggc
    32281 ctgctggttt atccatactt catagtgcag atgcggcccg gttgaacgtc cggtattacc
    32341 ggaaagcgcg atacggtcgc cacgtttcac cttctgtccc ggtttcacca ggatcttgcg
    32401 caagtgcata taacgcgtgg tgtagctgcg accatgacga atagccacat aataacctgc
    32461 tgcgccacta cgtttggcaa ccaccacttc accgtcaccc actgaaagca ctggcgtacc
    32521 ttgtggcatg gcaaaatcaa cacctctgtg tggcgcaacg cgaccggtca ccggattagt
    32581 acgacgcggg ttaaagttag atgagatacg gaactgtttc gccgtcggga atcgcaagaa
    32641 tcccttcgcc agaccagtac cgttacgatc atagaatttg ccatcttcag cgcggattgc
    32701 gtaataatct ttaccttctg aacgcaaacg tacgcccagc agctggcttt gctcacgttt
    32761 accatcaagc atttctcgtg acattaacac cgcaaattca tcgccttttt tcagtttgcg
    32821 gaaatccatt tgccactgca tggctttaat cactgcgctc acttcggcgc tggttaaacc
    32881 ggcgtttctg gcgctagcaa caaagcttcc cccgacggta cctttcagca gattgttgac
    32941 ccactctcct tgctgcattt cgctggtcat tttaaaaccg ttagcggcag tacggtcata
    33001 ggttcgggtt tcacgacgag acacttccca ggtgaggcgc tgcagttcgc cgtccgcggt
    33061 taatgtccag gagagttgtt gaccgatttt caggttacgc aattctttgt cggcagcagc
    33121 cagttgggtg atatcaccca tatcaatacc atactgattg agaatgctgc ttagcgtatc
    33181 gccagtggaa acaacatatt catgcacgcc cgcttcaccg gcgattttgt catccagttc
    33241 gtcctgggga atggcttcat cttcttgtgc agcttgatca atcggctcac tggcttcagg
    33301 taagagcgaa cgaatttcgt tctgttccag ctcaatggtt ttgacaattg gcgtggcatc
    33361 gcggtgataa acatagggcc gccagacagc gacggccaga gtaagaacgg tgagcgaccc
    33421 caacataaca cggtgtggtc gcggtaaatt attaaacgcc agggcgacag agcgggctat
    33481 ctgttgcacg taatcacttc ctcattaatc tcctttcagg cagctcgcat actggttggc
    33541 taattgattc aggaattctg aatagcttgt tttacccagt ttgatattcg tccccagggg
    33601 atccaacgtt cccatacgaa cggatgtccc tcgtgcgacg ctctcaacga ccgctggcct
    33661 gaactgtggc tcagcaaaaa cgcaggttgc tttttgctca accaactgtg ttcttatttc
    33721 atgtaaacgc tgcgcgccag gttgaatctc agggttaacg gtaaaatgac caagcggtgt
    33781 cagtccgaac tgtttttcga agtagccgta agcatcgtga aaaacgaaat aacctttccc
    33841 cttgagcggc gcgagctcgt taccaacctg cgtttcggtt gaggctaatt gtgcctcaaa
    33901 atccttcagg ttgtcgtcaa gtttggctcg actttgcggc ataagttcca ctaattttcc
    33961 atggattgca accgctgtag cccgcgctat ctctggggaa agccaaagat gcatgttgaa
    34021 atcgccgtga tggtgatctt cgtcactttt ttccgcgtga tcgtgatcat catcatcgtc
    34081 gtgaatactt ttcatcagca gcggtttcac atcttctagc tgcgcaatcg ttacctgttt
    34141 cgcttctggt aatttactta ccggtttttg catgaacgct tccatctccg ggccaaccca
    34201 aacgactaag tccgcgttct gtaagcgttt tacatccgat ggacgcagtg aataatcatg
    34261 ttctgaagcg ccgtcaggca gtaaaacctg cgtttctgtt accccatcag caatggcaga
    34321 agcgatgaac ccaacgggtt taagcgaagc gacaacggcg gcatctgcgg cctgtgttgc
    34381 accgccccag agagcggcgg ataatgctgc gaaaagaagc gtttttttat gtaacataat
    34441 gcgaccaatc atcgtaatga atatgaaaac tgtgatatta taacatttca taactactgc
    34501 aagactaaaa ttaacatgac aagtctggtt tccctggaaa atgtctcggt ttcttttggc
    34561 caacgccgcg tcctctctga tgtgtcgctg gaacttaaac ctggaaaaat tttgacttta
    34621 cttgggccaa acggcgcagg taagtcgaca ctggtacggg tagtgctcgg gctggtaaca
    34681 cccgatgaag gggttatcaa gcgcaacgga aaactgcgca tcggctatgt accgcagaag
    34741 ctgtatctcg acaccacgtt gccgctgacc gtaaaccgtt ttttacgctt acgccctggc
    34801 acacataaag aagatatttt gcctgcactg aaacgtgtcc aggccgggca tctgattaac
    34861 gcaccgatgc aaaagctctc gggtggcgaa acgcagcgtg tactgttagc gcgagcattg
    34921 ttaaatcgac cgcaattatt agtgctggat gaacccactc agggcgtgga tgtgaatggt
    34981 caggtggcgt tatatgacct tattgaccaa ctgcgtcgcg aactggattg tggcgtttta
    35041 atggtatctc acgatctgca tctggtaatg gcaaaaaccg atgaagtgct ttgcctgaat
    35101 caccacattt gttgttccgg cacaccggaa gttgtttccc tgcatccgga gtttatttct
    35161 atgtttggtc ctcgtggtgc tgaacaactg ggtatctatc gccatcatca taatcatcgt
    35221 cacgatttac agggacgaat tgttttgcgt cggggaaatg atcgctcatg attgaattat
    35281 tatttcccgg ttggttagcc gggatcatgc tcgcctgtgc cgcgggtccg ctgggttcgt
    35341 ttgtagtctg gcgtcgtatg tcttatttcg gtgatacgct ggctcatgcc tcattacttg
    35401 gcgtcgcgtt tggtttgttg ctggacgtga atccattcta tgcggtgatt gcagttacgc
    35461 tgctgctggc gggcggtctg gtatggctgg agaagcgtcc acagctggcg atcgacacgt
    35521 tattagggat tatggcgcac agtgccctgt cgctgggcct ggtggtcgtt agtctgatgt
    35581 ctaatattcg tgttgatttg atggcttacc tgttcggtga tttactggca gtgacgccag
    35641 aagatctcat ctctattgcg attggcgtgg tcatcgtggt ggctattttg ttctggcaat
    35701 ggcgcaattt gctgtcgatg acgattagcc cggatctggc gtttgttgat ggtgtgaaat
    35761 tacagcgcgt gaaattgttg ttgatgctgg tgacggcatt gacgattggt gtagcgatga
    35821 aatttgtcgg cgcgttgatt attacttcac tgctgattat tcctgctgct actgcacgtc
    35881 gctttgcccg cacgccggaa cagatggctg gtgtcgctgt tttggtgggg atggtggcag
    35941 tgactggcgg tttaaccttt tccgcatttt acgatacacc tgcaggcccg tcggtggtgc
    36001 tatgcgcggc actgttattt attctcagta tgatgaaaaa acaggccagc taatctttcg
    36061 ctgaacatat ttgtcggatg cggcgcgagc gccttatccg acctacggtt cgctatctct
    36121 ggtaggcctg ataagacgaa cagcgtcgca tcaggcacac tgccagtgtc ggatgcggcg
    36181 cgagcgacca atccgactta cggcatttct ggcggcgtta tgccaaagtg attccacgcc
    36241 cgcgtcgtcg ccatacgccc acgcggtgta cgctgcaaaa agccttgctg aatcaaataa
    36301 ggttccagta catcctcaat ggtttcacgt tcttcgccaa tggctgccgc caggttatcc
    36361 agacctaccg gcccaccaaa gaacttatcg attaccgcca gcaacaattt gcggtccata
    36421 taatcgaaac cttcagcatc gacattcaac atatccagcg cctgagcagc gatatctgcc
    36481 gagatggtgc catcgtgctt cacttcggcg aaatcacgca ctcgacgcag cagacggttg
    36541 gcaatacgtg gcgtaccgcg cgcacgacga gcaacttcca gcgcgccgtc atcactcatc
    36601 tcaagcccca taaagcgtgc gctgcgactg acgatatatt gcagatccgg cacctgataa
    36661 aactccagac gttgcacaat accaaaacga tcgcgcaacg gtgatgtcag cgaacctgcg
    36721 cgcgtggttg caccaatcag ggtaaacggc ggcaaatcaa ttttaatgga gcgtgccgcc
    36781 ggaccttcac caatcatgat atccagttgg taatcttcca ttgccggata caacacctct
    36841 tccaccactg gtgaaagacg gtggatctca tcaataaaca gtacatcgtg tggttcaagg
    36901 ttagtgagca ttgctgccag atcgcccgcc ttttccagca ccggaccaga agtcgtgcgt
    36961 aaattaacgc ccatttcatt ggcgacaata ttggcaagcg tagttttacc caaccccgga
    37021 ggaccaaaaa tcaatagatg atcgagggca tcgccgcgca gtttcgctgc tttgatgaaa
    37081 atctccatct gcgaacgaac ctgcggctga ccaacatact cttccagtaa tttagggcga
    37141 atggcgcgat ctgccacatc ttccggcaaa gtggtaccgg cagaaatcag acggtctgct
    37201 tcaatcatcc tttacctcat aacgcggcgc gtagggcttc gcgaattaat gtttcactgc
    37261 tggcgtcagg gcgagagatt ttgctcacca tgcggcttgc ttcttgtggt ttatagccca
    37321 gtgccaccag cgcagcaacc gcttcctgtt cagcatcgtc ggtcgccggg ctggcaggag
    37381 acgtgagtac caggtcggcg gctggcgtaa agagatcgcc atgcaaacct ttaaatcggt
    37441 ctttcatttc gacaatcaag cgttcggcgg tttttttgcc aatacccggc agtttcacca
    37501 gtgcccccac ttcttcacgc tcaacggcat taacgaactg ctgcgctgac attccggaga
    37561 ggatcgccag cgccaacttc gggccgacgc cgttggtttt gatcaactct ttgaacaacg
    37621 tgcgctcttg tttattgtta aaaccgtaca gcagttgcgc gtcttcacgc accacaaagt
    37681 gggtgaaaac gatcgcttcc tgacccgctt cagggagttc ataaaaacag gtcatcggca
    37741 tatgcacttc atagcctacg ccgcccactt caattaacac cagcgggggt tgtttttcaa
    37801 tgatgatgcc tctgagtctg cctatcacat gacgctcctg cgtaatgaat caaagataat
    37861 gctgtatgat aaaaaaatgc tggatagata tccagcgaag gatgaagaaa acttgcgagg
    37921 tgtctcgatg atctgaaaaa tggcgcagta taatttattc tacaggttat attggaagca
    37981 aatatttaaa tattacatat tcagcgaaga aatgtgtaat aaaaatacac atcgcgaccc
    38041 ctgaaaaaaa aataaatttt ttatgctatt acgtatattc atatctattt caatggaatg
    38101 acaacgtgaa tattaattat cctgctgaat atgaaattgg tgatatcgtc tttacatgta
    38161 taagtgctgc cttatttggt caaatatcag ctgcatcaaa ttgctggagt aatcacgtcg
    38221 ggatcattat cggtcataac ggtgaagact ttctggttgc agaaagccgt gttcccctct
    38281 caaccatcac tacgctatcc cgttttatta aacgctctgc taatcaacgc tatgctataa
    38341 agcgattaga cgccggacta acagaacaac aaaaacaacg aattgttgaa caggttcctt
    38401 cccggctacg caaactttac cacaccggtt ttaaatacga atcttcgcgc cagttctgtt
    38461 caaaatttgt ttttgatatt tataaagagg cgctatgtat tccggtgggt gaaatagaga
    38521 tgtttggaga attgttaaat agcaatccaa atgcaaaact cactttctgg aaattctggt
    38581 tcttaggttc tattccgtgg gagcgtaaaa ccgtcacgcc agccagtttg tggcatcatc
    38641 cgggtttggt gttgattcac gcggtgggag ttgaaacgcc tcagcctgaa ctgaccgagg
    38701 cggtataact taacgcagtc gccctctcgc caggttcagt cgcgattcgc tcatttgcat
    38761 cgcattctga ctaacgtggc agtgggtgat ggcaatcgcc agcgcatcgg cggcatccgc
    38821 ctgtggatta gcgggcagtt tcagcaaggt gcggaccata tgctgcacct ggcttttttc
    38881 ggcactacca atacctacca ctgtttgctt tacctgacgt gccgcatatt caaataccgg
    38941 caattcctga ttcaccgccg ccacaatcgc cacgccgcgc gcctgcccca gttttagggc
    39001 tgagtcagcg ttcttcgcca taaagacctg ttcaatggcg aaataatcag gctggaattg
    39061 ggtgatgatt tccgtcacgc ccgcatagat gagcttcaga cgagacggta aatcatccac
    39121 tttggtgcgt atgcatccgc tacccaggta ggacagttgc ctgcctacct ggcggatgac
    39181 gccatagccg gtcacgcgcg aacccgggtc aatgccgaga ataatagcca tcacgcgtct
    39241 ccgttttgct gtttagcagg cctcatcaga gagtcgctgc aacctcatca gagatttcac
    39301 cgttatggta aacttcctgc acgtcgtcgc aatcttccag catatcgatc agacgcatca
    39361 gtttcggtgc ggtttctgca tccatatcag ctttggtgga cgggatcatg gaaacttccg
    39421 cgctgtctgc tttcagacct gccgcttcca gagcgtcgcg tactttgccc atttcttccc
    39481 atgcagtgta gacatcaatc gcgccgtcat cataggtcac aacgtcttca gcaccggctt
    39541 ccagggctgc ttccatgatg gtgtcttcat cgcctttctc gaaggagatc acgccttttt
    39601 tgctgaacaa ataagctacg gaaccatcag taccgaggtt accgccacat ttgctgaatg
    39661 catgacgcac ttcagcaacg gtacggttgc ggttgtcaga cagacattca atcatgattg
    39721 ccgtgccgcc aggaccgtaa ccttcgtaga tgatggtttc catgtttgca tcatcatcac
    39781 cgcccacacc tcgtgcaatt gcgcggttca gagtgtcacg ggtcatgttg ttagacagtg
    39841 ctttatcaat tgctgcacgc aaacgcgggt tagcgtccgg atcaccaccg cccagcttag
    39901 ccgcggttac cagctcacga atgattttag tgaagatttt accgcgctta gcatcctgcg
    39961 cagctttacg atgtctggtg ttggcccatt tactatgacc tgccataaaa atatctccag
    40021 atagccctgc ctgttcaggc agcgttaatt acaaactgtt caatcgcctg ccggttgctc
    40081 caggacttag tgagcgccgc cgcagcagac gcatcaagcc acttgtaagc cagatgttca
    40141 gtgaaaacga tctggcgctc gtgcggaagc gcaagacaga accatgattc cgtattacgc
    40201 gtcacgcccg gcgcatagcg atgacgtaaa tgtgaaaaaa tttcaaactc taccgtgcgc
    40261 tgacagtcaa ttaaggtcag ttgttcagcg acaacatcaa tggtgacctc ttcctttact
    40321 tcgcgcatgg cagcttgcgg cgcggtttca ccctcttcca cgctgccggt taccgactgc
    40381 cagaaatcgg gatcgtcacg ccgctgcaac atcagcaccc gtttcgtatc ttgtgcgtag
    40441 atgaccacta agatcgaaac gggacgctta taagccatat cagttattct cagccttctt
    40501 cacaacctga atgctcagct cagccagtgc agtcaggtta gcaaagctcg gtgcttcagt
    40561 catcagacac gctgccgccg tggttttcgg gaaggcgata acgtcacgga tattgtcggt
    40621 gccggtcagc agcatcgtca gacggtcaag accgaatgcc agacctgcgt gcggcggagt
    40681 accgtatttc agggcgtcga gcaggaagcc gaatttctcg cgctgttcct cttcgttgat
    40741 acccagaata ccaaacaccg tctgctgcat atcaccatta tggatacgca cagaaccacc
    40801 gcccacttcg taaccattga tgaccatatc gtaagcgtta gccaccgcat tttccggtgc
    40861 agctttcagt tctgctgccg tcatatcttt cggtgaggtg aacggatggt gcattgctgt
    40921 caggccgcct tcaccgtcgt cttcaaacat cgggaagtcg ataacccaca gcggtgccca
    40981 tttgctttcg tcggtcagac caaggtcttt acccactttc aggcgcagtg cgcccatcgc
    41041 gtcggcaaca attttcttgt tgtcggcacc gaagaaaatc atatcgccat cttgcgcgcc
    41101 agtacgctcc aggatggctt cgatgatttc tgcattaagg aacttcgcta ccgggctatt
    41161 gataccttcc agacctttcg cgcgttcgtt aactttgatg taagccagac ctttcgcgcc
    41221 gtagatttta acgaagttac cgtattcgtc gatctgctta cgggtcaacg atgcgccgcc
    41281 cggaacacgc agagcggcaa cacggccttt cggatcgttc gccggacctg caaatactgc
    41341 aaactcaaca gatttcagca gatcggcaac gtcggtcagt tccatcgggt tacgcagatc
    41401 cggtttatca gaaccataac ggcgttctgc ttctgcaaag gtcattaccg ggaaatcgcc
    41461 cagatccacg cccttcactt ccagccacag atgacgcacc agcgcttcca tcacttcacg
    41521 cacttgcggc gcggtcatga aagaagtttc cacatcgatc tgagtaaatt caggctgacg
    41581 gtcagcacgc aggtcttcgt cacggaagca tttaacgatc tgatagtagc ggtcaaagcc
    41641 ggacatcatc agtagctgtt tgaacaactg cggggattgc ggcagcgcgt agaatttacc
    41701 tttgtgcaca cgagaaggca ccaggtagtc acgcgcacct tccggcgtgg ctttggtcag
    41761 catcggagtt tcgatgtcga ggaagccgtg gtcatccata aaacggcgca ccaggctggt
    41821 gattttagcg cgggttttca ggcgctgagc catttccggg cgacgcaggt cgaggtagcg
    41881 gtatttcaga cgcgcttctt cggtgttgac gtggttagag tcaagcggca gaacatctgc
    41941 acgattgatg atagtcagcg aggacgccag tacttcgatt tcgccagtcg ccatatcgcg
    42001 gttaatattt ttttcgtcac gcgcacgtac ggtgcccgtg acctgaatgc agaactcatt
    42061 acgcagttca gaggccagct ttaacgcgtc cgcacgatcc ggatcgaaaa atacctgcac
    42121 gataccttcg cggtcgcgca tatcgatgaa gatcaggcta ccaagatcac gacgacggtt
    42181 gacccaacca cacagagtca cctgctgccc cacgtgggac aaacggagct gtccacaata
    42241 ttctgtacgc atgagatatc ccttaactta gctgccggcg gatgccccct gctgcgcagg
    42301 tgaccaagtc gcagcgttag ctgtatgtca caactgaatg aaaaaaggcg gctattatac
    42361 tggaaattct gccgcaccgt aagagcctgg cccgcgctgg aacgcctcgt taccacttta
    42421 tatcgggcct gaaatcagac tctacgccag tttgctataa aggtgttgcc cgaactcata
    42481 aaaattaaca aaatttgtcg ttccgccatc ggctaatcgc attaaggtga gaggcacgat
    42541 tttgttttgt caggagtcat catgcctgaa cttaatgcta aaaccaccgc gctggtggtg
    42601 attgatttac aagaaggcat cttacctttt gccggaggtc cacatactgc cgatgaggtg
    42661 gttaatcgcg ccgggaagct ggcggcgaaa tttcgcgcca gcggtcagcc cgtgtttctg
    42721 gtgcgcgttg gctggtctgc cgattacgcc gaagcattaa aacagccggt tgatgccccc
    42781 tcccccgcaa aagtgttgcc cgaaaactgg tggcaacatc ctgctgcatt aggtgcaacc
    42841 gacagcgata tcgaaatcat caaacgtcaa tggggtgcgt tttacggtac ggatctggag
    42901 ttgcaattac gccgccgggg tatcgataca atagtgttat gtgggatctc gaccaatatc
    42961 ggtgttgaat ccaccgcccg caatgcctgg gaactcggtt ttaatctggt gattgccgaa
    43021 gatgcctgta gcgccgctag cgccgagcag cacaataaca gcattaatca tatctacccg
    43081 cgcatcgccc gtgtgcgcag tgtggaagag atcctccacg cgttatgatt tacatcggtc
    43141 tgccgcaatg gtcgcatcct aaatgggtgc ggttggggat caccaacctt gaagagtatg
    43201 cccgccactt taactgcgtg gagggtaaca ccacgcttta tgccctgccg aaacccgagg
    43261 ttgtcctgcg ctggcgtgag cagaccacag atgacttccg cttctgtttt aagtttccgg
    43321 cgaccatttc gcatcaggca gcattacggc attgcgatga tttagtgact gaatttttga
    43381 cccgcatgtc accgttggct ccgcgcattg gacaatactg gctgcaactg cctgccacat
    43441 tcggcccacg ggagctgcct gcgctttggc attttctcga ttctcttccc ggcgaattta
    43501 attatggtgt ggaagtccgc catccacagt ttttcgccaa agaggaagag gaacaaacgc
    43561 ttaatcgcgg tttacatcag cgcggcgtta atcgggtgat tttagacagc cgcccggttc
    43621 atgcagcacg tccacacagt gaagctattc gcgacgctca acgaaaaaaa cctaaagttc
    43681 cggtacatgc tgtactgacg gcgaaaaatc cactgatccg ttttatcggt agtgatgata
    43741 tgacgcaaaa ccgggaatta tttcaggtct ggttacaaaa attagcacag tggcatcaga
    43801 ccactacgcc ttatcttttt ttacatacgc cagatattgc ccaggccccg gaactggtac
    43861 ataccctgtg ggaagactta cgtaaaacgc ttccagagat cggagcagtt ccggctattc
    43921 cacagcaatc ttctcttttc tgaatttgcc acctatcata gacaggtgcc atcggccatt
    43981 ttaaagggag tttgtatggt aagcgcgctg tatgccgttt taagtgcgtt gttattaatg
    44041 aagttctctt ttgatgtcgt tcgcctgcga atgcagtacc gcgttgccta tggcgacggc
    44101 ggttttagcg aactgcaaag cgctattcgc attcatggta acgcggtgga atatattcct
    44161 atcgcgattg tgttgatgct gtttatggaa atgaatggcg cagaaacctg gatggtgcat
    44221 atttgcggca tcgttttgct tgctggtcgt ctgatgcatt attacggttt tcatcaccgt
    44281 ctgttccgct ggcgacgttc tggcatgagc gccacctggt gtgcgctgtt gctgatggtg
    44341 ctggcgaatc tttggtatat gccctgggag ttggttttct ccctgcgtta gcgcacaata
    44401 cgccactttc tttttcccgg atttttacgt tatgtctcac cgcgacacgc tattttctgc
    44461 ccctatcgcc agactgggcg actggacctt tgatgaacgg gtagctgaag tcttcccgga
    44521 tatgatccag cgttccgttc ccggctattc caatattatt tccatgattg gtatgttagc
    44581 cgagcgcttc gttcaacctg gtacgcaggt ttacgatctg ggttgttctc tgggcgcggc
    44641 gacgctctcg gtgcgtcgca acattcatca tgataattgc aaaattattg ccatcgataa
    44701 ctccccggcg atgattgaac gctgccgtcg tcatattgac gcctataaag ctcctacgcc
    44761 agtagacgtt attgaaggtg atattcgcga tatcgccatt gaaaacgcat cgatggtggt
    44821 gctgaatttt accctgcaat tcctggaacc ttccgagcgc caggcgttac tggataaaat
    44881 ttatcaaggg ctgaacccgg gcggtgcgct ggtgctttcg gaaaaattca gtttcgaaga
    44941 tgccaaagtt ggtgaattgc tgttcaacat gcaccacgac tttaaacgtg ccaacggtta
    45001 cagcgaactg gagatcagcc agaaacgcag catgctggaa aacgtgatgc tgaccgattc
    45061 cgtggaaacc cataaagcac gcctgcataa agccggtttt gagcatagcg agctgtggtt
    45121 ccagtgcttt aactttggtt cactggtggc attaaaagca gaggacgctg catgatcgac
    45181 tttggtaact tttattctct gattgccaaa aatcatcttt cacactggct cgaaacgctg
    45241 cccgcgcaga ttgctaactg gcagcgcgag cagcagcacg ggctgtttaa gcagtggtcc
    45301 aacgcggtgg aatttctgcc tgaaattaaa ccgtatcgtc ttgatttact gcatagcgtg
    45361 acggcagaaa gcgaagaacc actgagcgcc gggcaaatta aacgcattga aacgctgatg
    45421 cgcaacctga tgccgtggcg caaagggccg ttctcactgt atggcgtcaa catcgatacc
    45481 gaatggcgtt ccgactggaa atgggatcgc gttctgcccc atctttctga tttaaccggg
    45541 cgcaccattc ttgatgttgg ctgcggcagc ggttatcaca tgtggcgcat gattggcgca
    45601 ggcgcgcacc tcgcggtggg gatcgaccct acgcaactgt ttctgtgcca gtttgaagcg
    45661 gtgcgtaaac tactgggcaa cgatcagcgc gcacatttgt taccgttagg tattgaacaa
    45721 cttccggcac tgaaagcctt tgataccgtc ttttcgatgg gcgtgcttta tcatcgtcgt
    45781 tcaccgctgg agcatctctg gcagttaaaa gaccaactgg tgaatgaagg cgaactggtg
    45841 ctggaaacgc tggttattga tggcgatgag aacacggtgc tggtgccggg cgatcgttac
    45901 gcacaaatgc gtaatgtcta tttcattcct tccgcgctgg cgctgaaaaa ctggctgaag
    45961 aagtgtggtt ttgttgatat tcgcattgca gatgtgagcg ttaccaccac agaagagcag
    46021 cgacgcaccg aatggatggt caccgagtct ctggccgatt ttctcgaccc gcatgatccg
    46081 ggtaagacgg tggaaggtta tcctgcgcct aaacgcgcgg tgctgattgc gcgcaagccg
    46141 taaaggtctg gtaatactgc cggatgcggc gtgaacgcct tatccggcct acaaagtctt
    46201 gctaattcaa tatattgcag gggctatgta ggcctgataa gcatagcgca tcaggcagtt
    46261 ttgcgtttgc ataacctcag cgcccgtttc cgggcgcttt tcacgtctta tgcctgaaca
    46321 gctggctgat caaacgccgt taacttcagc gcattgcccg tatatttttc gatatacacc
    46381 agcgcagagt taccggcaca ggcattcgcc agctgcgagc tggggatatc cgccgttaac
    46441 acgttcgcac taccgttttt acacaagcca ttttccagat ctggccatgc accttcatgc
    46501 acgcatacca cgcctttttt gatcccgtca gtcaccaccg cgcctgtcag gatctgaccg
    46561 agcttgttcc agacacgcac cagatcgcca ttcgcaatac caaagcgagc agcatcttcg
    46621 gtgtgaatag tgattggttc acgatcggca accgcatatt ttttacgcag ttccgcatag
    46681 ttaagctgac tgtgtaaacg gtgtgccgga tgtgcggtca gaagctgcaa ctgcttctcg
    46741 tcggcggtac ccttccactc atcaggcgca agccaggttg ggtgtgccgg gcaatcctta
    46801 tagccaaatt tttccagcgt tctggagtaa atctcaattt tgccgcttgg cgtacccagc
    46861 gcatttttca ccggatcggc gcggaaatca ccataacgaa tgtactgttc gttcttctcg
    46921 ctgcggcgca tttcgatcag tttattttgc tgccagaagg cattaaacat cggcatagtc
    46981 acgcgttgcg cacgggcacc tttctgagcg gcatcataga aaaatttcag ccacgccatt
    47041 tcatctttac cttcggtata gatctctttt ccgccaggtt tgagtaattc agcaagatcg
    47101 gcaaacacgt caaaatcgtt gcgcgcttca aattgcgcag cgacagcctg cttcatcggc
    47161 acaatatgct ggttgctgta atcaccggtc atcgtcaggt cattgcgctc aaacgatgtg
    47221 gtgatcggta atacgatatc tgcgtgtttg gctgctgcgg tccagtagca ttcagaaacg
    47281 acgatcatct ccggtttctg ccatgcttta atcagacgat tagtatcctg gtgatgggta
    47341 aagttaccgc cacccgccca ccagatcatt ttgatattcg gatacgtttg ttctttaccg
    47401 ttatgctgat atttgccgct aggattttcc agcgcatcga cgatacgggc aacaggaatc
    47461 gccgtcattc cgccatcatc agcggcctcg ctggcctgtc cggcaatcgc cgcagacatt
    47521 tctggtagca cgccgccaac acgcgtcggg ttgccaccgt tggagtaatg ataagagaaa
    47581 ccaaacgcgc cgcccggtgt accaatctgc cctaacatcg ccgccagcgt caccagcatc
    47641 cagtgtttct gttcaccata ttgctggcgc tgaattcccc agcctgccat cagcattgtg
    47701 cgatttgccg ccatcagttc agcgagtttg actatctgcg cttccggtac gccagtaatt
    47761 tccgccgccc aggccgcgct cttcggcgta ttatcgctct tacctgtcag atactcttca
    47821 aactgcggat acccggtagt gtatttttca aggaacactt tatcgtgttt gccttgtgtc
    47881 atcagggtat gcgcaatccc taacatcagt gccacatcgg tgcccatatt cggtgcgatc
    47941 caggtggcat tatcgccaaa gaattcgatg gtttcggagc agataggatc aatggcaatc
    48001 actggtttgc cagatttttt cagctgatgg aagtattcca gcccttgctc atcggtactg
    48061 ctccaggcaa ttttgagcgt gttcagcggg ttcatccccc acagcaccac cacctggctg
    48121 ttttccagaa taagcggcca ggaggtctgc tgttcataca cctctacaga accgaccaca
    48181 tgcggcatga tcacctgtgc cgctccggtt gaataatcgc cgctatgccc ggaataaccg
    48241 cccgccaggt tcatataacg ttgcagtaag gtttgcgctt tatgcaacac gccagaagaa
    48301 cgccagccgt aagatccggc aaaaatggcc gatggtccgt tagctttacg aatacgatcg
    48361 tgttgttcat gaatcagttt taatgcttgt tcccagctca cctgtacata ggtatcttcg
    48421 ccacgacctt tcgccggttg cagtggatta tcgagatagc tttttctcac catcggatgc
    48481 tgaatacgcg ccgtggtgtg tacctgatcc gccgccgtag actgtaagga gttcggtatg
    48541 gttttcgcca gcgcgcctgt cgaagaaaca atcttgccgt ccttcacttc tacgttcatc
    48601 gctccccaac ggcccgcagt gagaattttg ccgcccttct cttctgccca tgcgggtagc
    48661 ggttcagccg atgtcaccac cagcgtccct gccgcaatac cgctatgttt aataaattca
    48721 cgtcttgtta atgtcataac ttcctccctg atcaacgagg atcactgttt ctcggtaata
    48781 tctttggcgt tgtactggaa gtaccgcgtc aaaatgtcca gttcgttttc gctcatgctg
    48841 gttcgcgccc ccattccttt ggcaatggac ggccatgcat tgacggtgta atggtcggcg
    48901 gcaatagggg catggcagcc agcgcaatag gtatcgtcaa gtttttcagc gtattgccat
    48961 agcggtttac ggtccgctaa tgcgggatcg ataagcgccc cctgcaaaga cgcctgacgc
    49021 cattgattgc cgtattcgtc agcctgccat tccccgttta ccgttagcgc cttgatacct
    49081 tcttcactta atgtggcgag cgccagccgt tgacctgccg ccaggtagag cgtgttttca
    49141 ctgccctgca tttgataacc ctgcaaccga acaattggct gtttaccact gacatcaacg
    49201 acggtaagat cggtaccagg agtcacggta gccaggtcgc ctatgcgaga agttttgaaa
    49261 ggataaatat gtgcgccatt agtaactgaa gtagcggcct gactttccag ctcatgcgcc
    49321 gcgttgtcat ccatttttat ttctggcgga aaatgggcaa tgcctttatg gcaatcgata
    49381 caggtttcgc cgtccttttg cgctttatta tgcatttttt gcgcagattc actttgcgag
    49441 gcaatatcca tggcatcaaa agaatggcaa ctacggcagg ttgcagagtc agttgctttt
    49501 aattctttcc atactgtttc ggccatttcc tggcgatgag cttcgaactt atcgtcactg
    49561 tctattttgc cgctaacaaa ttcatgataa atatctttag atgcttttaa tttagcaaat
    49621 aaataatcca tccctgactt tggaatatgg caatcagcac attccgcacg tattcctttc
    49681 tggtttgaaa agtggacagt tccctgatat tcctcaaaag gtttactcat cgagtggcag
    49741 gaaaggcaaa atgcggtatc cgacgtttta tgtaaggctt tttgcgccag caataacccg
    49801 ccgccaccaa ccacaaccgc tatcagcaaa aacaataacc caatgcgttt tttccctcgc
    49861 atagattcct ctaattaaat tccaggacac cgataccatt gtataaataa atacacaacg
    49921 aattgagtac ttgcttaatt cggcacaaca ctgcctgaaa taatcgataa agaatatgat
    49981 ttattacaat gtaatcatta attgctaaga aataacccag ttgttattta gaatatatat
    50041 ttgtcagttt tggcaattta aatcacataa tgaatgtgaa tatataaaat atttttagca
    50101 caacgctatg cgcactgtca cccgctcaac agatcacggc aatcgttcgt tttttatact
    50161 gctcagggat aaaaaaaggc ccctgttgaa attgcagggg cctggtacga gcaagcatca
    50221 tattgggcga catgatgcaa cggtaaaaat catttggcct gatggcattc aatgattcct
    50281 ttcatttcag caaccgccgc cccgtctacg acataacgcg aatactcgtc cgcgtgtgca
    50341 tctgatgaca tggacaatcc ttgattacga taacgcatcg gtgaggcttg ccacgctccc
    50401 gcggagctat ggacttccag cactccggca tcgaggaagt ggtgcaggtt ttccgcacgg
    50461 actcctgctc cggccataat gattggagca tcacgatggg caataagttc cataattttt
    50521 gataaacctt gcagtgcgtc tgatttttgc cctgatgtca gtacccgggt aatgcccaat
    50581 tccgcaagat tattgagtgt atttaaaggg ttagcgcaca tatcgaaggc gcgatgaaaa
    50641 gtcactgcca gcggaccggc agcagccatt attttttcca ttcgtggcat atcgacattc
    50701 ccgtcaacat cgagaacgcc cgtcaccagt ccaggaaaac ctaattcgcg gactgtgcgc
    50761 acatcttcaa gaatggcggc aaactcaccg tcgctgtagc aaaaatcacc accgcgtggg
    50821 cggataatcg gatgcactgg gatcgtcacc cgctggcgca cgaatttcag tacccccaac
    50881 gacggcgtta agcccccctc ttttggagct gcgcataatt caactctgtc agcgccgttt
    50941 tgctgcgccg ttagcgcgca ttccatgctg taacagcaaa tttccagtaa tgccattttt
    51001 gctccttagt tacgccgact gctcgctggc aacgatctct tcaatggacc acggatgaaa
    51061 cttaatggtt gttttaccat cggtgaccgc cagcgtcgga ttcggtaatc gttcatgctc
    51121 accttttggc gaactggttt taactgagat ccccggcagg ctgagtcctt catcagaaag
    51181 caatgccagc gcacgggcat tcagcgtttc tggatccccc ggcagaacga tttcaatatg
    51241 ttcccaacct tcgtgcgggt aacgtttttc cccgggccac ggtagctcca caatggaaaa
    51301 ctgccagtgc gcaacctgta ccggttcatg caatttaaac agacaaatcg gtctgccatt
    51361 gatcatattt tctgacaaaa gttcgccgca ctgttcaaac ccgcgacgcc agcgttcagc
    51421 ggtgacgttt tgatggcaac gcaaagaaat gtgatcggca gtcagcggag tgatatccaa
    51481 ccccagacgg cgggaaagtt catctaatgc gtgggtaaat cgcggtaaat ccgatgcaat
    51541 atcctgcagc tcgtcgatag attgccagtt cgccataatc actcttcgtc tttcattaaa
    51601 agcgctaatt taccctgttg ccctgcgcca accaaccgct gatttcacgc cgcttctgat
    51661 gcaatagtga aaacggcaat acgccacgcg cacgttgctg acgaaaacag ccatttgcag
    51721 tatactcccg ccctaatttc tttaactggt gcgggcaatt tttgctcgct tcatcaatgt
    51781 aaggtattcc ggtgaatatt caggctcttc tctcagaaaa agtccgtcag gccatgattg
    51841 cggcaggcgc gcctgcggat tgcgaaccgc aggttcgtca gtcagcaaaa gttcagttcg
    51901 gcgactatca ggctaacggc atgatggcag ttgctaaaaa actgggtatg gcaccgcgac
    51961 aattagcaga gcaggtgctg actcatctgg atcttaacgg tatcgccagc aaagttgaga
    52021 tcgccggtcc aggctttatc aacattttcc ttgatccggc attcctggct gaacatgttc
    52081 agcaggcgct ggcgtccgat cgtctcggtg ttgctacgcc agaaaaacag accattgtgg
    52141 ttgactactc tgcgccaaac gtggcgaaag agatgcatgt cggtcacctg cgctctacca
    52201 ttattggtga cgcagcagtg cgtactctgg agttcctcgg tcacaaagtg attcgcgcaa
    52261 accacgtcgg cgactggggc actcagttcg gtatgctgat tgcatggctg gaaaagcagc
    52321 agcaggaaaa cgccggtgaa atggagctgg ctgaccttga aggtttctac cgcgatgcga
    52381 aaaagcatta cgacgaggat gaagagtttg ccgagcgtgc acgtaactac gtggtaaaac
    52441 tgcaaagcgg tgacgaatat ttccgcgaga tgtggcgcaa actggtcgac atcaccatga
    52501 cgcagaacca gatcacctac gatcgtctga acgtgacgtt gacccgcgat gacgtcatgg
    52561 gtgaaagtct ctacaaccca atgctgccgg gtatcgtggc ggacctgaaa gccaaaggtc
    52621 tggcagtaga aagtgaaggc gcgaccgttg tattccttga tgagttcaaa aacaaggaag
    52681 gcgaaccgat gggcgttatc attcagaaga aagatggcgg ctatctctac accaccactg
    52741 atatcgcctg tgcgaaatat cgttatgaaa cactgcatgc cgatcgcgtg ctgtattaca
    52801 tcgattcccg tcagcatcaa cacctgatgc aggcatgggc gatcgtccgt aaagcgggct
    52861 atgtaccgga atccgtaccg ctggaacacc acatgttcgg catgatgctg ggcaaagatg
    52921 gtaaaccgtt caaaacccgc gctggcggta cggtgaaact ggccgatctg ctggatgaag
    52981 ccctagagcg tgcacgtcgt ctggtggcag aaaagaaccc ggatatgcca gccgacgagc
    53041 tggaaaaact ggctaacgcg gttggtattg gtgcggtgaa atatgcggat ctctccaaaa
    53101 accgcaccac ggactacatc ttcgactggg acaacatgct ggcgtttgag ggtaataccg
    53161 cgccatacat gcagtatgct tacacgcgtg tattgtccgt gttccgtaaa gcagaaatta
    53221 acgaagagca actggctgca gctccggtaa tcatccgtga agatcgtgaa gcgcaactgg
    53281 cagctcgcct gctgcagttt gaagaaaccc tcactgtggt tgcccgtgaa ggcacgccgc
    53341 atgtgatgtg tgcttacctg tacgatctag ccggtctgtt ctctggcttc tacgagcact
    53401 gcccgatcct cagcgcagaa aacgaagaag tgcgtaacag ccgtctgaaa ctggcacaac
    53461 tgacggcgaa gacgctgaag ctgggtctgg atacgctggg tattgagaca gtagagcgta
    53521 tgtaatcgcg ttatacggca acagccgatg tcattgcaga catcggcttt tttatcatta
    53581 acgataattt acaatcactt cattccgctg aatctttagc ggcggaatta accgcccacc
    53641 gccaggcact tcccagataa agcgcaacgg ttctgccgcc ggtataccgg aaaaggccac
    53701 ggtggttcca ctctgcccct ctaattcgac acaacgagat tgtgaacaca agcgaacccg
    53761 cagtcctgca ggtgtcggac cgataagctg ataacgccac gccaccagcg tcatcaatcc
    53821 tgaagcaggt tgtcgcgtag aaagaggcgc tgacgacatc gattcaccgc gatgatttag
    53881 cgtaacgccc acgctactcg cctgccacat cccctccccg gcggcttgca ccagcagcgg
    53941 aaacaataat attgctaata aggctctcat tatttgccgc caatcgtcgc tgtcatacgg
    54001 atatgtcggt tatctgatag ttccagattc gacagcacca ctaactgcgg caagctgcgg
    54061 cgcaggaagc gagacaataa tggtcgcagc gcgtggttca ccaacagtac cggcggcgca
    54121 cccaacatct cctgacggga tagcgcttcc tgagtttgcg ccaacaagcg atccgccagc
    54181 cctggctcca gtcccccccc gccctgcaat gcctgtagca acaaacgttc cagcggtgta
    54241 tcgaggccaa taacatggac ttcatctttg ccaggaaacc actgctgggt aatcgcacgt
    54301 cccaacgcca cgcgcacgac agcggttaat tcatgcggat cgctctggat gggcgcatgt
    54361 tccgccagcg tttcgagaat ggtgcgcata tcgcgaatcg gcactttttc atcgagcaga
    54421 ttttgcagca ctttatgcag tgtggtaagc gtgacgacgc caggaacgag atcttccgtc
    54481 agctttggca tctcctgggc gacgcgatcc agcagctgtt gcgcctcctg acgaccaaac
    54541 agctctgcgg catgctggct aatgaggtgg ttaagatgcg ttgccaccac cgtgctggcc
    54601 tcaaccacgg tgtacccctg aatctgcgcc tgctctttaa gcgcactttc aatccagata
    54661 gcattcaggc caaatgccgg atcgacggtc gcctcaccag gtaacgtccc ggcagcggtt
    54721 ccagggttaa tcgccaacca acgccccgga taagcatcac cactgcctat ctccacgcct
    54781 ttcatcaaaa tgcgatagcg ggcaggttgc agatccatat tgtcgcgaat gtgtaccact
    54841 ggcggcagaa accccatctc ctgggcaaat ttcttgcgga tactgcgtat acggcccaac
    54901 aactcaccat cctgctggaa atcgaccatc gggatcagtc gataacccac ttccattccc
    54961 agagaatctt ccagttgtac atcgttccac gtcgcttcga caacggtatt attctctgcc
    55021 atgtttaccg gttttgcttc ggcaggcgct ttttgttcac gtccgcggat ccaccacgcc
    55081 agccccagca atccggcagt gaacagcaaa aataccaggt tcggcattcc aggcaccagg
    55141 ccgagtaaac cgagcacggc ggcgcttaat aacataacgc tcggattact gaaaagctga
    55201 ttcaccatct gctcgccaac atcctgatcg gtgctgacac gtgtaacgat gacgcccgcg
    55261 gcggtagaaa tcaccagtgc cgggatttgt gccaccagac cgtcgccaat ggtcaatagc
    55321 gtataacttt ccgccgcgtg ccccatgctc atgccatgtt gcagcacacc gaccagcaat
    55381 ccgccgacaa cgttaatgac catgatgagg atcccggcga tggcatcgcc gcgaacaaac
    55441 ttacttgccc cgtccatcga gccgtaaaaa tcggcttcct gagtcacttc ggagcggcgt
    55501 tttttcgctt catcttcacc aatcaatccg gcgttaaggt cggcgtcaat cgccatctgc
    55561 ttacccggca taccatcgag aacaaagcgc gcgcccactt ccgcaatacg cccggcacct
    55621 ttggtgataa ccataaagtt gatgatcacg agaatgacga acaccacgat accgatagcg
    55681 aaattgccac caacgaggaa gtgaccgaac gcttcgacca cctttccagc cgccgccgcg
    55741 ccggtatgcc cttccatcaa aatgatacgg gttgaagcca cgttaagtgc cagacgcaac
    55801 agcgtggtaa acaacaggat ggtcggaaac gcagcaaact cgagcgtgcg ctgggtaaac
    55861 atcgccacca gcaacaccat gatcgacaag gcaatattga aggtaaacag caggtcgagt
    55921 atgaatgcgg gcagtggcag taccatcatc gacaagatca acaggatcaa aatcggtccg
    55981 gcaaggatct gccattgtgt cgatttcagg tttgcaggca ggcgcagcat cgcggccaga
    56041 ttactcatgg gtcggtttct cgttaataaa atccagggct tccggcaccg gaagatgagt
    56101 aggttgtaca gggcgctgtc caccggccag acgccagcgt ttcagttgcc agacccaggc
    56161 cagcacttcc gccaccgcgg cgtacagttg acccgggatt tgttgaccga tctccgcatg
    56221 tcgatacagc gcacgcgcca gcggcggcgc ttcaagcgtc gggacgttat tttcagcgcc
    56281 aatttcacga atgcgcagcg cgaccaaccc tgcaccttta gcgaccactt tcggtgcgct
    56341 cattttgttt tcgtcatact gcaacgctac cgaatagtgg gtcgggttat tgacaatgac
    56401 atccgctttc ggcacatcgg ccatcatccg acgccgtgcg gccgctcgct gcatctgacg
    56461 gatccgccct ttaacatggg ggtcgccttc gctttgtttg aactcatcac gaatatcctg
    56521 ccgtgacatt cgcagctttt tcaggtggct gaagatttgg aaaaagacgt caaatcccac
    56581 cattggaatg acaccaagca ccaccagcag cgcgcatagc cctaccagat ccatcgcatt
    56641 acccatggcg gtaatcggag actcggccat taagcgcatc atctgcggcc agtgatgcca
    56701 gagaaaaaac cccgtcacgc tgccaaccag gatggttttc aaaattgcct taagcaactc
    56761 cgcgccagtc tgtgccgaga acatccgttt aatgcccggt agcgggttga gtttggaaaa
    56821 cttcggctgc aaggattttc cgctaaatac cagccccccc agcataaccg gagaaataat
    56881 cgccaccagc accacgccgc taatcagcgg tagcagcgcc agcatggctt ctctgatcag
    56941 cagaataatc tgcccgagga tcagattcgg gtcattgata atactgtgat caaaatgcag
    57001 ccctgcggag agcatgcccg acaatcgacg ggccagcgac acaccgccaa accagataac
    57061 actaacgccc accagcaaaa tcagcagtga agtcagttcg cgggaacgcg ggatttgccc
    57121 ctcttcccgc gctttttcta gtcggtgagg tgtgggggct tctgttttgt cgtcgctctc
    57181 gtcagacacg tcgccaatcc tggaaagagt attaagcgta aatgatgcca gagcgcaaag
    57241 tgttcaatgg tttgagtaag gggcaaaaca ggcgggattt agggcttttg ccacagcaca
    57301 tcaggcatgg tgacatgttt gtcgggtacg gcgtaaacgc cttatccaac cttgaagatc
    57361 tgatgtgacg cggccacgtc acatcaggca atacaaatca aaatccaaga ctatccaaca
    57421 aatcgtccac ctgatcctga ctggctacca caccggctat gctggtatcg acctgaggtc
    57481 cattaagcaa actctggttt tcacgttttg gacgcgactc ctgttccggg atgttttcca
    57541 acagcaccat cagcaactga cgttcgatct cctgaatgac atccatcatc cgcttaatga
    57601 cctgtccggt gagatcctga aaatcctgcg ccatcatgat ttccagcagt tgcgcgttag
    57661 taaagctggt atgtgcgggg acatctgcca gaaattgccg cgtgtctgtt accagttcac
    57721 gggcgtcggc aaggtcaatc ggatcggcaa accagtcatc ccaacgttgg gttaacgctt
    57781 ttgctgattt ctccatttga tcctgatgcg gttgtgacgc ctcaacactg ttcagcgccc
    57841 gctccgcagc ctgggcggtc atctgcacaa catagtacaa acgatcgcgc gcatcgggga
    57901 tagcttccgc tgcttcggca atggcctgat ccagccccag ttcccgcaaa ctgtcgcgca
    57961 gcatacgcgt caggctgccg atgcgcgcaa tgatatcgcc agctgaatgc tcgtcagcag
    58021 gtttgattga tggttgcatc atagtcgcat cctcacatgc ccagtttctc aaagattttg
    58081 ttgagttttt cctccagcgt cgcggcggta aatggtttca ccacatagcc actggccccc
    58141 gcttgcgccg cagcaatgat gttctctttc ttcgcttctg cagtcaccat taacactggc
    58201 aatgccgaca tcgcgccatc cgcacgaatc gttttcagca actccaggcc atccatattg
    58261 ggcatgttcc agtcggagat aacaaatcca taaccgcctg cctgcaactt attgagagcg
    58321 tcgacgccat cttccgcttc ctcaacatta ttgaatccca gctctttcag caggttacgc
    58381 actatgcgtc gcatggtgga aaagtcatcc acaaccaaaa atttaagttc tttatccgcc
    58441 atttcacact cctgatttaa atacgtatcg cctgtccggc actaattttt gccagcattt
    58501 gctgacttac ctggctaaga tcgaccactt cgcagacacc acccatattg atggcctcgc
    58561 gcggcatgcc gaacaccacg caacttgctt cgttttgcgc aagggtccat gcccccgcct
    58621 gacgcatcgc caacattccc gccgcgccgt cgttgcccat accggtcagg atcaccccaa
    58681 ccgcattacg ccccgcctgt ttggccacag aatggaacaa cacatctacc gatggccgat
    58741 ggcgattaac cgccgggcca tcgtgaattt tgatttgata atttgcgcca ctacgcgcta
    58801 gctccatatg ccgatcgccc ggcgcgatat aggcatgtcc cggcaagacg cgctctccgt
    58861 cttcggcttc tttaaccccg atctggcaaa gcttattaag tctgtcggca aaagagcggg
    58921 tgaaaccggg cggcatatgc tgggtaatta acagtgccgg actggaaagc ggcaacggtt
    58981 gcagtacgtg acgaattgcc tcagttccac ccgttgaagc accaatcgca atcagttttt
    59041 cagaactcaa caacggccct gccttcagcg ttgtcggtac cgacaatggc ttatgtgctg
    59101 caaggctcgc ctttgctgct gtacgcacct tttcagcaat catttcgcta tacgccagca
    59161 ttccttcgcg aatacccagt tgcggtttgg tgacaaaatc tatcgccccc agctccagcg
    59221 cgcgcagcgt gacttctgac cctttgccgg tcagggaaga aaccatcact acgggcattg
    59281 gacgcaaacg cattaatttt tcgaggaaat ccagtccgtc catccgcggc atttcaacat
    59341 ccagcgtcaa tacatcggga ttgaatttct taatcaaatc acgcgcaacc agcggatcag
    59401 gggcggtcgc caccatttcc atgtcgctat ggctgttgat gatttctgtc atgatttggc
    59461 gcatcagtgc cgaatcatcg acagataaca ccctgatttt gctcatcgtt aatccttact
    59521 tagcgcatac accgtctgac cacgcagcgt gaagcggcgc tcaaggtggc taaagttttc
    59581 agagtgaccc gcaaacagca atccgtcggg tttaaggagc ggaacaaagc gacgcaaaat
    59641 ctcctgctgg gtagtttgat cgaagtagat catgacgtta cgacagaaga tcgcatcaaa
    59701 cggccccggc acggtgtact gtttcgccag taaattcagc ggggcaaaat caacatagtt
    59761 cgccagctcc tgacgcacgc gtaccagccc ttcatgcggt cccgtccctc gcatgaaata
    59821 ccgttgcagt tgctgcggcg tcaggttttt caactcttca tggcgataaa taccgcttct
    59881 ggctttttcc agcacttcgg tgtcgatatc gctggcaaac actttccagc gtccgggcac
    59941 ggtgctcaat gtttcagcca gcgtcatcgc aatgctgtac ggctcttcgc cggtcgaagc
    60001 cgctgcgctc catacgcgat actcgccaga acggcgacgt gcgtgatccg cgagcagagg
    60061 aaaatgatgc gcctcacgga aaaatgccgt cagattcgtg gtcagcgaat tgataaacgc
    60121 ctgccactca ccgctgtgct gattagattc cagcaagttc aggtaatggc cgaaatccgt
    60181 cagtcccagc gaacgcaaac gacgaaccag tcggttgtaa accatgtcgc gtttatggtc
    60241 ggccaggacg atcccggctc gttgatagat caattgactt atccgccgaa aatgcgcgtc
    60301 ggaaagcgcc aggcgctcgg tcatctgtaa caataaagac gtttgcccac agggcagaga
    60361 tgaagtcata gcgccttctc aatcacttca ggataccact ggcgcaattt gtaactgcgc
    60421 cgactcatgt cgtgctactt catgttcttc aagggtaaat accgccacac gcgacgaaag
    60481 atggtcggcc tgattcagtc gttgcaaatc gtcgcgaaat gcccaaaaga catgccgtta
    60541 ctgccaatct gcaagatccc gcagagaatc aaaattaaaa acagcgtggt cgaaattcga
    60601 atacgattaa acatcaacgc tccccatcag gcggcaatga ccgctttagt aaatactcgt
    60661 caaaatgttt cccagtttgg atcttgttca gcaattcgca gtcgtggctg tgccggtggt
    60721 tgctcactgg caggacggga tggtgtttgc ggtttattgg tgagtgggct ggctgccaga
    60781 cggaacgcgg aaaccgcttg cgttaaacga ctcgcctgtt cttccagcgc agctgcggcg
    60841 gcagctgatt cctgcaccag cgatgcgttc tgttgcgtga cgcgatccat ttccgaaacc
    60901 gccaatgcga cttgatcgat gccacggctc tgttcatccg atgctgatgc aatctcgccc
    60961 ataatgtcag tcacgcgagt gacagcattg acgatattgt tcattgtttc cccagcgctt
    61021 tcgaccagca ccgaaccggt atcaacgcgc gagacggagt cttcaatgag ggctttgatc
    61081 tcttttgccg cctgtgcact gcgactggca agattacgca cttcacccgc caccacggca
    61141 aaaccacggc cctgttcacc cgctcgcgcg gcttcaacag cggcattcag cgcgaggata
    61201 ttagtctgga aggcaatacc atcaataacg ctgataatgt cggcaatttt cttcgaacta
    61261 tcggcgatct catgcatcgt tttcactacg ccatccacca ctttgccgcc gtgctgggcg
    61321 gtgtcggagg cactttgcgc cagttgcgag gcctggcggg cgttatcggc gttttgcttc
    61381 actgtcgcgg tgagctgctc catgctggcg gcagtttctt ccagcgcgga tgcctgctgt
    61441 tcagtacggg aggaaagatc ggtgttgccc gccgcaattt cacgggtacc ggcatagatg
    61501 gcatctgaac cttcgcgcac atgagtgacg gtgtcagtca aagagcgttg catatgtgaa
    61561 acgctctgcg ccaggtcgcc catttcactg cgcccgtcaa tggtcagggt attcgccagg
    61621 ttaccaccgg caatttcgcg aatatgcgca ataatttttg ccagcggtgt aagcaacata
    61681 cggcgaatgc cgtaccacgc caccagcaga atcaatacca ccaccagcgc gataaccgcc
    61741 agttgccact gggcaaatcg gtaatcatct gcgttgtcag tgacgatatc gcgatacagt
    61801 ttttcactgc tgagggcgta ctgagcaaac gcttcgccca ttgcattttg cattccctgg
    61861 gttggctgag cgaaataagc tccagtattg ccataatcaa gataatcaat cagttcagtt
    61921 aacgctgtgt gatagttttt atatttttca tcaatattac gactggtagc gaccatttca
    61981 ggtaacggtg ccatgctttt gaatttttta taatgagtcg cggcctgggc caatgttttc
    62041 ctggcgctat cgagcaattc aactttggca ttactttgct gattggatga atccatcatc
    62101 atccgcaccg ctgaacgact caggttaatg cgcgtttgta gcattaaatc ccatgttgac
    62161 gtcagctcgc cctgctgttc ccgtaattga ttggaaacca caaagctctt ctggctatgg
    62221 tgaagggaag aaaaaaacag actgccggaa ataagctgta acagtgcgaa tacccccagc
    62281 accattacca acagcgtgac tacgcggata cggttaatca taaggcacct tcctgaaaac
    62341 atgttgatct cgttatcggc aaggaggggg gaaactttat tgctgatgcc acccgccgcg
    62401 aaattgaaat aaaaaacccg atgcgcagat catcgggttc atttcaattg aggaaatcgg
    62461 gagaattacg ccacttctga cgccgcgcta tctaacagcg ccatctcttc gctgttcagc
    62521 agtttttcga tgttcaccag aatcaacatg cggtcgccca gtgcgcccag tcctgtgaga
    62581 tattcggttg aaagcgtcac ggcaaattcc ggtgccggac gaatttgctc cgccgtcaat
    62641 gaaagcacgt ctgagacgcc gtcaaccacg atgccgacca cccgctgtcc gagattcagg
    62701 acgataacta ccgtgttgtc gttatagtcc acatccacct ggctgaactt aattcgtaag
    62761 tcaacaatcg gcacaataac gccgcgcaga ttcgtgacgc ctttgataaa cgctggcgtg
    62821 ttcgcaatcc gtgtcacctg atcgtagcca cggatctcct gcactttcag aatatcaata
    62881 ccgtactctt catcaccaag ggtaaatacc agaaattcct ggcctgacgg ctcgctggcc
    62941 agctttgtta cattcgtcat accggtcata ttgttacctt tttactcatt caggcggcgg
    63001 tgttcgccat acgttgttcg cggtttatcg cctgcaaggc ggagacatca acaatcagtg
    63061 ccacgctgcc gtcgccaaga atggtcgcag cagaaatgcc ggggactttg cgatagttac
    63121 tttcaaggtt tttaaccaca acctggtgtt gaccaattaa ttgatccacc agcaaggcgt
    63181 agcggcgacc gccactttgt aagatcacca caattccctg ggtggcttcg gttttcgcgc
    63241 ccgcgacgtt gaacactttc cacagttcga cgataggcag atattcaccc cgcacttcca
    63301 gcacccgctc gccgccagcc agtggatgca gatcggcttc acggggttgc agtgattcca
    63361 tgacagcatt cagcggcaga atgaaaactt catccgcaac gcgtacggac atgccgtcaa
    63421 gaattgccag cgtcagcggc agtaaaatgc ggatcgtagt gccagtaccc tgcttcgact
    63481 ggatctcgac atgaccgccc atctcctgga tattacgttt aacgacgtcc atgccgacgc
    63541 cacgcccgga gacgtcggtg acctgctccg ccgtggagaa gccaggtgca aatatcagca
    63601 ttgcaacttc gtcgtcgctc atgttttcgc tgacggtcag accttgcgag gccgcttttg
    63661 ccagaattcg ttcacggttt agccccgccc catcgtcggt cacttcaatg caaatgttgc
    63721 cgccctgatg ttcggcagac agaattaaat ttccgacgct gtttttacct gcagcgagcc
    63781 gtttttctgg cagttcaata ccgtgatcga ggctattgcg taccaggtgg gtcagcgggt
    63841 cgataatgcg ttctatcagg ctcttgtcga gttcagtgga actgcccacc agcgtcagtt
    63901 ctacctgctt gccgagtttt cccgccagat cacgcaccag ccggggatag cgactaaaga
    63961 catattccat cggcatcatg cgaatcgaca tcactgattc ctgcaaatca cgagcgttac
    64021 gttgtaactg ccccatgctg gtaatcaaat caccatgatt aaccgggtcc agttcactgg
    64081 agcgctgagc aagcatggac tgggtgatga ccagctcgcc gacgaggtta attaattgat
    64141 caaccttttc taccgctaca cggatgctgg tggattcact gctgcgcgtc gttttttccc
    64201 gctccacgcg accggttggc gcttgttcgg ctgccagttt aagcactggt ggggtggata
    64261 tttttggcga gacgtctact gtttcaaagg taatctgatc ggcttcaatc acaaaacaga
    64321 gtaccgcagt gatgtcatct tcggcgatgt cgtccggtaa tattgccgag agagaatcag
    64381 cccctttcac cacgtcagtt aacgttgtca gatgccccaa ctcttcttcc agcaggtcga
    64441 cttcgccggc cttcaggcgc gaaaggataa ttcgtcgcgg tgactgacta cgactctgct
    64501 catcttgcgg ttcacttttg gcaaccacac ttaatcgggt cactgcagat ggcgtttcgc
    64561 ctttcgcttc taatgccagt tgacgcaagg cctggcagat ataatcgaag ctggcggcat
    64621 ccgtctcttg cgactgttta taagcgtcga gctgttcttg catgatgtcc ttcgtttcca
    64681 aaaacagatt gataatgtcg gtgttgagtt gcatctcacc tcgtctggct tcatcgagca
    64741 ggttttccat cagatgcgtg gtttcctgca aaacgctgaa gccaaaggtt cctgcccctc
    64801 ctttgatcga gtgggcagcc cgaaagatgg cattcaactg ttcggcatct ggcgcttccg
    64861 gctgcaaaac cagcagatgc tgctccatat cagccaacag ttcgtccgct tcatcaaaaa
    64921 atgtctgata aaaatcgctt atatccatgc tcacgctgtc acctcggttc ggctgatggc
    64981 attgtgggaa cactgacctg tggtgcaacc tcaggttttt ccagggcgct tactggctca
    65041 ttctggcttt cggcgttttc atgcaaaatg gcctgttcgg cgtgtttgtt cagtaccagc
    65101 aggctgatgc gacggttgac ggcatcatca ggtccgcgat cgcttaagcg catcgttgcc
    65161 gccatgccga cgacacgtaa cactttgccg ccattcaacc caccaaccat cagttcgcgg
    65221 cgggatgcat tggcacgatc ggcagaaagc tcccagttgc tgtaaccttt ctcaccgctg
    65281 gcgtagggaa aatcatcggt atgacccgag aggctaattc ggttgggaat accgttcagt
    65341 acgggcgcga tggcgcgcag aatgtcgcgc atatagggtt cgacgtcggc actgccagtt
    65401 ctaaacatcg ggcgattctg gctatcgatg atctgaatac gtagaccttc ctggaccaga
    65461 gcgattttga gatgtggacg taacgcccgc agtttcggat cggactcaat caactggtcg
    65521 agatcgccac gcaatttccg caatcgactt tgctccatgc gttttttcag ctcttcgatg
    65581 ttcggctgct tattcacttc cccctggctt tgggtgtaat catcaccccc accgggaatt
    65641 gggctttcac tattagaaat gcgatcgccg cccgtaaccg ctgtcgccag cggagtccgg
    65701 aagtactccg caatctgaat cagctctttt ggactggaga tagagatcag ccacatcacc
    65761 agaaaaaagg ccatcattgc ggtcataaag tcggcataag caatcttcca cgatccatgt
    65821 gctgccccgt ggcttttggc tttgcgtcgt ttgacgacaa taatcggatg cgcttgattc
    65881 ttcatgcttc ctcggttgtc gtctgttgtt gcggattttt caccgcacgc acatgctctt
    65941 ccagctcaat aaacgacgga cgttcgctgg aatagagcgt tttgcgacca aactcaacgg
    66001 ctataggcgg tgcgtaaccg ttcagatttg aaagcagagt gactttgacg cactgcatca
    66061 ttttgctggt ttcggcgctt ttctgacgta aaacactcgc taatggggaa ataaatccgt
    66121 aagccaataa aataccgagg aaagtcccca ccatcgcatg agcgataagc gcccccagct
    66181 cggcggcagg acgatcggct gaacctaacg cgtgaactac ccccattaca gccgcaacaa
    66241 taccaaacgc cggaagtgag tccccgacca gcgccagact gttcgccggg acttctgcct
    66301 cgctttcgtg cgtctcaatc tcttcatcca tcagagcttc gatttcgaag gtgttcatgt
    66361 gaccactgat aatcaggcgc agataatcga cgataaattc aagcatgacg ctatccgcaa
    66421 ggatgcgtgg gtagctggcg aagatctcac tctcacgggg attttcaata tcgcgttcca
    66481 gcgaaaacat ccccatctgc cgcgatttcg ccatcaaccg ataaagcaga gccagcagat
    66541 ccatatacat tgctttggtg tatttggagc gacgaaacag caacggcagc gccttcagcg
    66601 tgcctttaat cgctttgcca ttattgccga cgataaacga cccaatccct gcaccggcaa
    66661 taatcaccag ttcagcgggt tgatagagtg ctccaaggct tcctccggtc atcaaataac
    66721 cgccgaaaac tgtaccgaga acaaccaggt aacctaataa gataagcacg acatcatcct
    66781 tccactgttg accatgacag gatgttcagt cgtcaggcgt taacacgcga tagggacaaa
    66841 aaaaagcagc ggtacatcgt taccgctgct ggaatgtttc gccacaccgt atcagttaaa
    66901 cagcctgtac tctctgttca tccagcagtt gtgggataat atcggcagga ttctgggaaa
    66961 gtttacgtct ttttactgcc cgggatggcg gctgacataa gctgcaggca aagctgccaa
    67021 caggctggtg agcatgggta ataaaattgc cgccgcagca gttgcagctg gaaagttgca
    67081 gtaatccact ttcaacaaac cgcaccaatg tccaggcacg ggttaatgcc agcagcggtc
    67141 cttcttctgc ttgcgggcac tgttcaaggt ataaacggta ggctttgatc accgcatcga
    67201 cgccattaca caagccagtt ttcagtaaaa actgccatgc attacagaac atcgaagcat
    67261 gaacgttttg ttcccaggtc ataaaccagt cggttgagaa tggcagcatg cctttcggcg
    67321 gtgggcttcc gcgcagttct ttatagagtt ttatcagacg tccgcgactt aactgtgttt
    67381 cgctttccag catctgcaaa cgagcgccca gggtgatcaa ttccattgcc agctgaatat
    67441 cccgcgcttc ctgaacaatg cttttttcac tcatgatcag gcccttttct tacgcagcgc
    67501 ttcttcaggc tgattaacat cattcagcaa gcgtgttgag agcatgatgc cggtatgaat
    67561 ttgctggaga tcgtcaacgc gggaatcttg cgtcaactga gtaatcgtct ggtggctgtc
    67621 aaaacggaag tgacaaacca gttgattggt ttctgccagc ttaaccattt gcggaagagt
    67681 cagtgccgct aacgttgtcg ccatttcttc atttatgccg agacgaaaca tagcggacgc
    67741 tttgtcctga acaatcagac gctgtgcaag tagtaaatat gacaagttga tgtcataaat
    67801 gtgtttcagc aactcggagg tatgcattat tcccacccag aataaccaac tttattttta
    67861 tgcggtctca ccgcaccccg tgatgtcgcc gggaagcccc ggtaaaaaat aattagcatt
    67921 agaatagttg cgataagctg caataagcag acccaccttt ttggtttaat aagtccttac
    67981 aaatagaagg gggtctttac acttatctaa gatttttcct aaatcgacgc aactgtactc
    68041 gtcactacac gcacatacaa cggagggggg ctgcgatttt caataatgtg tgatgcagat
    68101 cacacaaaac gctcaattac ttaacataaa tgtataagtc atacttttgt tttgggtgta
    68161 tttcaatctg ttaaaaaatt tttcgctaca ctggcaagca aaaatgaaac aggaataatc
    68221 gaaatgggat gttgcgcaca gttaaaataa ctcaccgtaa ataatcatct gctataaata
    68281 atcgctttaa tgcaatacca gataagccat ttttaaacaa acacttaccg cacaacaaac
    68341 taattaacta caaagaaagg caacaatcaa cctaatgtta ataaaatgtt ttacatatat
    68401 taaccacttg ttcaatatgg gaaatcagta cattattttc ttataaatac agttgaaatc
    68461 atatgataac caagagcaat tgattaattg tatttactaa tgaccagact aatatttacc
    68521 tgtttaactg agttgggatt gcgtcgtttc tccattagga gtaaagcttt aatgtcacct
    68581 gaagttcaca gaataaggaa caggttatga gctatagcaa tattcttgtc gccgttgcgg
    68641 ttacaccgga aagtcagcaa ctgctggcaa aagcggtatc tatcgccagg ccagtaaagg
    68701 gacacatcag tttaattact ctcgcttccg acccggaaat gtacaatcaa ttagctgcgc
    68761 cgatgcttga agatttacgc aatgtgatgc aggaagaaac gcaaagcttt cttgataagt
    68821 taattcagga tgcggggtat cccgttgaca agacgtttat tgcctacggt gagttaagcg
    68881 aacatattct ggaagtttgt cgcaaacatc atttcgattt ggtgatttgc ggtaatcaca
    68941 atcacagttt cttttcgcga gcatcctgct cggcgaaaag agtgattacc tcaagtgagg
    69001 tcgatgtgct gttagttcca cttacgggag attaaccgca cctacgcaag cttaggaaag
    69061 gtagcaactt tatcgcgctg ctggctttcc gcgcttcgcg gaactatctg ctttaggtcg
    69121 ctaatgaagc actcctgcca gtggttaata tcgtttttca cgataacgtc cagcatttct
    69181 gcatgacggg aaatacgttc cgccagcgac atagtcaatg cacgatccag cgcagctgca
    69241 acttcgtcac gatcgtaggg attaacaatt aacgccgacg ttaactcgtt tgccgctccc
    69301 gcaaattgcg aaagaacaag aacgcccggg ttggctgggt cctgagcggc aacatactct
    69361 tttgcaacca ggttcatccc gtcacgcagt ggcgtcacta agcccacgtc agagtagcgg
    69421 aatattttca tcagtaattt acggtcaaaa tgctgattca aataataaag cggcgtccag
    69481 cctaattgcc catatttacc attaattcgt ccggcttcat tttcgagctg atgacgaata
    69541 tcctgatagg cttgcacatc accacgagac gttggcgcga tctgggtata acgaatttta
    69601 ccgtgatgtt gcggatattt ttccagtaac gcttcatagg cgagaaaacg ctccggcaaa
    69661 cctttggaat aatccagtcg ttcgacagaa aagatatttt gtacgttttt cagttctgct
    69721 ttgagttgcg ccagttttgg cggcagtgga ccggcagcct gtttcgctat ttctttcggc
    69781 tcaatgccga tcgggtaaac ttccgttcga aacgctttgc cgcaggccgt atggcttttt
    69841 gcgctacgtg tcgtgacgcg ggtcaggtta gaaagacaat ccaggaacgc cagacgatcg
    69901 ttctccgtct ggaaacccag caaatcataa tcacaaagct gttcaagcaa ggtgtcatat
    69961 gtcggcagcg cgttgaagat ttccggtgtc gggaaaggaa tatgcagaaa gaaaccaatg
    70021 cgattattca cgccccgttt gcgtaattca tgcgcaaagg gcaacaggtg ataatcgtgg
    70081 atccagataa tgtcatcgtc ttgcaacagc ggcagtaatt tatctgccag caacgcattt
    70141 acgcgtagat agccatccca ggcaggacgc tgaaattgca ccagatcgag ccgataatga
    70201 aaagcgggcc agagaacggc attggagaat ttgttgtagt attcgtcaag gtcctgttcg
    70261 ctgaggttaa aagaggccca cgtaatgtta ccctttttca ccttttttag cggctgatcc
    70321 tcattccctg tttcaccgct ccagccaaac cacagtccac ctgcggcttt cagtgccccc
    70381 agtatgccaa cggcaaggcc accggcactg gcggcgtgct cgtctggtgg tgcaatccgg
    70441 ttagatacta cgactaaacg actcatagtc atcactcctg ttattttctc ttttttgttg
    70501 taatgcggtg gttatcattt caagccagct ccagacatcc ggcacgcccg ccagtcgcca
    70561 tgatgcctga gttgcacctg tgccaatttt tactgacatt ccgcccagcc ggttaacgac
    70621 tgcgaagcca gattcatcgg tcagatcatc gcccagaaat acgggcgttc gcccgataaa
    70681 gggagcttcc tgcataaagg cagcaatcgc ctcaccttta ctggtacctt tcggtttgat
    70741 ctcgacaaca cactttccct gctgtaacgc catttgtggc cagatctgag taatacgttg
    70801 cgctaatgtc atcaatgcgt cttcatgctg cggagcctgg cgataatgca gcgcaaaagc
    70861 catccctttc gcctccagct ccgcgccggg atactgggcg atgaccgtat gcagttgcac
    70921 gctaatatca cgcgcaatcg catccggcag atgaacgata tgcgttttac cattgatgtc
    70981 acggcgctcc gcaccatgca cgcccgctaa cgggaagcga taaggttttg ccagtgcgtc
    71041 aagctccacc attgagcgcc ctgaaatcaa tgccaatgca ccatcacttg cggttgccag
    71101 tagctgaagt ccttgcagaa tagtgtcagg cacgacgacc tgatcgggat gcggtttgat
    71161 ttccgccagc gttccatcga gatcaaaaaa ccaggcatat ttcgcggata gttcaggggt
    71221 ttcagttaac ggttctgtca cccggttctc ctccttctct tttcttattg tgttgcgcag
    71281 aacgcgcttt acagacatgt aagtatagac agtgtgacgg gggtcgccat ttgcaacgaa
    71341 gaacagccaa ccgttgtggc ggctggctgt ggtgggaaaa aacgctaaat tgttgtggaa
    71401 aaaagcatca gacagtgcgt ttcgcttttt gcttgtaacg gtcgaaaatc accgctgcca
    71461 gcaggattaa gccgcgaact acgtactgtg cgaaaggaga aatattaagc aggttcatgg
    71521 cgttttccac ggtgcctaaa attaagatac ccgccaccac atatgagatt tttccgatgc
    71581 cgcctttcag agaaacgcca cctaaaacgc aggcggagat cacaatcagc tcatagccaa
    71641 tcgacgtcat tggctgccca ctggtcatac gtgaagccag aataattccg gctatcgctg
    71701 ataccaggcc agagagaaca aagataataa ttttggtgcg aacaaccggt acacccgcca
    71761 gacgcgcagc ctcttcgttc ccgccaatcg ccagggtgtt acgcccaaag gtggttttat
    71821 tcagcagcaa gccaaagatg atcagacacg cgacggtgag ccagattggc gcaggcagac
    71881 caaaccagtt ggcgtaacca agggcaaaga agctttcatc ttcgataccg accgctttac
    71941 cgtctgaaat gatgtacgcc agacctcgaa caatctgcat cgttgccagt gtcgtgatca
    72001 gggcatttat tttgagtttg gcgataacaa agccattgac cagaccacag agaatgccca
    72061 gcagcaaccc cgctgccacg ccaatccaca ggctttcagt cagattaata accaccgccg
    72121 tggtgacacc cgcacaggca attacggagg cgacagaaag gtcaaagtca ccggaagcaa
    72181 ggcagaacaa catgccacaa gccaccatcc ccgacatgga aattgccagg cccaaccctt
    72241 tcatattaat gaaggtggca aaatttggga caaaaatggc acaggcgata aaaagcaccg
    72301 caaacaccac cagcatgccg tactgatccc agatacgccc gaagctgaat gacgacttag
    72361 gtgcgccaga acccgatgta gaaacagaag acatcatact ctccttactc aggcaactgc
    72421 ctggctgact ttaggcatcg caaggctcag tgcctgacgc tcatctgcct gctcgtgtaa
    72481 caattcaccg gcgatttcac cttcgcgcat caccacaatc cggtcggcaa cgccgaggac
    72541 ttcaggtaag tcgctggagg caaacagcac cgccacaccc tgcgccgcca gcgcataaat
    72601 cacgttatat atttcgtgct tagcaccaac atcaatgccg cgcgtaggtt catccagcaa
    72661 aatgaccttc atctcttccg ataaccagcg gcccagaatg gctttttgct gatttccacc
    72721 tgagagattc ataatcagtt gctcagcgcc cggcgttttg atgttgagcg aacgaatgtg
    72781 gtgatcggca ttgttttctt cccaaccgtt gttgattaca caaccgccga gcacatgttt
    72841 gcgtctggca ctgatgttga tattgtcgcg aacggagtgc acaggaataa tgccttccgc
    72901 tttgcgatct tccgggcaga gcatcatgcc tgcggtaata gcatggctcg gtttacgaat
    72961 atcgatcggc tgttggtcga tataaacctg accggcggtg atttgcgtac cgccaaacat
    73021 gcctttcatt aattcgctac gccctgcccc gaccagacca aacagaccga caatttctcc
    73081 actgcgaacc gccagactta ttggcgtacg cacgcctggt gctttcacag catcaagacg
    73141 cagccgctcc tcgccataac tacgcggttg ccagccgtag atatcgccaa tgtctcgccc
    73201 gaccatcgcc tgcaccagtg cgtcgtggtc aacctgctgc atatcggtaa aggttttgac
    73261 ataacgccca tctttaaaga cggtgatggc atcgctgagg gcaaatattt cttccatacg
    73321 gtgagaaacg tataagatca cccgcccctc ttttcgcagt tcacgaataa cgcggaaaag
    73381 attgtcgatt tcgcgggcag agagggagct ggttggctca tcaaaggcga taattttggc
    73441 gttacgcgcc agcgctttgg cgatttcaac catctgccac tgaccaatgg agagatattt
    73501 cagcggcgtg tccgggtcaa tatccatacc aagatgttta atttgtaaac ctgcctcata
    73561 attcagcaat gaacgattca caatgccgcc tttatgcggc agctggccga gatagatgtt
    73621 ttccgcgacg gtcatttccg gcacgagatg cagttcctgg taaataatcg ccacccccgc
    73681 gttaagtgct gcggtcgtgt cggaaaagga catttcctgc ccattaatca ctacagaacc
    73741 cgtggttggc gcatagtttc cgctgaggat ttttaagaga gttgattttc ctgcgccatt
    73801 ttcacccatc aacgcatgaa cctgaccggc atagcagtca aaactaatat ccgtcagcgc
    73861 cttaacgccg ggaaacgttt taccgatgcc gcgaaatgag agatacgggg tagactgttg
    73921 cataacgtct ccgtgaatca ctggtcgttc acaccccctc tgcatcatgc agaggggaat
    73981 ttttccggca aattacttac cgcctaaacc ttttttctcc agttcttctt taaagttgtc
    74041 acgcgtgatc agcaccacgt cggtaacttc ggtgaatttg gtcggttcaa caccttttgc
    74101 aacccagttg taaagcattt cgctggactt ataaccatgt acgtccgggc ttggcagcag
    74161 ggaaccgtag aagccggttg cctgtgcctt agacagttca ctcaccgcat ccacaccgtt
    74221 aataccgata ccgatgatat cgctcgcttt aaagccctga ccttccgtcg cgcgtacgcc
    74281 gcccagcacg gtgctgtcgt tcataccgac aatcagccaa tgtttaactt ccggatgttg
    74341 aaccagcatt gagttggcag cgtcaaatgc ccccgggatg tcgttagatt tggtaggtac
    74401 ctgataaatt tgtttttccg ggaatcctgc agctttcagc gcatccatag accccgtagt
    74461 acggcgacga gccgtatcca gttcgttgga ggtaatcgcc atcaccgcgc tttctttgac
    74521 atcccagcca cgtttctgca tctctttata cagttcctgg ccctgacgtt cgccaatttt
    74581 ggtcgcagcc atcatcacca gcggaacggt atccattggc ttaccttttg cgttaacaaa
    74641 ctggtcatcc acggcaatga ctttcatatc gtagccacgc gctttcgcca cgatggcaga
    74701 gccgagtttc gggtccggag tacaaataac gaaacctttc gcgccactgg cagccaggct
    74761 gtcgatagcg ttcaatgttt tttcgccatc cggtacggca atcttaataa cttcaaaccc
    74821 taaatccttc ccggctttat cggcaaactt ccattcggtc tggaaccacg gctcttccgg
    74881 ttgcttcacc aggaaaccga gcttcaggtt ctccgccata gcggattgtg acataacggc
    74941 tgccagacca atggctgcca gggctttagt aaatttgtgc atggttctct ccagctttag
    75001 tgtcgttttg tgtagggcaa aaacgaatga cattcgttaa attaatcgga aaacaaagca
    75061 ttacctttta actaaaagat aagtgactgt gttgacatag ttttagcgag aaattaattc
    75121 tccataggag agcaatatca catcgcagaa ttacagtgag aacgtgcata aatttagcgg
    75181 gaaaagacat aaggaaaagc gaatttgtca gacaaattat cgagtgcaca gcagaataat
    75241 ccataagatt agcctggaag tccttgttgt ctttggtacc catgcgggat gttttctttt
    75301 taaccagtca ataggcctca ttacctggcg ttgagttttt gcaatggtgt aataaccgca
    75361 acttaaagat gtggaaaatg cgcgtcattc atttcgccat taattatcac tgtactcatt
    75421 aattaacaga acacgtataa tgagagccat atcgcaaaaa tgaaaaaacg ttttataaaa
    75481 tcattgcttc atcatgaatt caaattcatc gattaatatc aacaagatac aaaaagcacc
    75541 atcattaaaa ttcattacag ttacattgat ttcatcaatg aaatgtaaaa atatataaac
    75601 ttgatgattt aagcattttc ttatacccgt tcagacgtta ttcttatttc agatcatcgt
    75661 cagaattgac tccacgatca catttcggac cggcagaaag gaattattct gcaaacagta
    75721 attatggtgt tttgatttat cttgcacctc tccacttctg gatataagga tattaggtat
    75781 ggcaaccgct ggaatgcttc tcaaactcaa ctctcaaatg aaccgcgagt tttacgcatc
    75841 caatctctac cttcacctga gtaactggtg ttctgaacag agtctgaacg gcaccgccac
    75901 tttccttcgc gcccaggcac agagtaatgt gacccaaatg atgcgcatgt ttaactttat
    75961 gaagagtgtc ggcgctaccc ccatcgttaa agccattgat gttcccggtg aaaaactgaa
    76021 ctctctggaa gaactgttcc aaaaaacgat ggaagaatac gagcaacgtt caagcacgct
    76081 ggcgcagtta gccgatgaag cgaaagaact gaatgatgat tcaaccgtca atttcctgcg
    76141 cgatctggaa aaagaacagc agcatgatgg cctgttactg caaaccattc ttgatgaagt
    76201 gcgcagtgcg aaacttgctg gtatgtgtcc tgtgcagacc gaccaacatg ttctgaatgt
    76261 cgtgtcgcat cagctgcatt gatcatacat cggcgccaat gcattgcgcc gatgaagttt
    76321 ttgagaaacc gctgcctcat ttttttgaag cagcggtttt ttaatgggat tcgccctgtg
    76381 gagtaaactt gagttcaatc aacgcgatgg ctttttggat tgcccgcatg gtgaccgggt
    76441 ctgcggcggc gggatggtta gtaaagtcga tattcttcag ctggctggac attttttcgc
    76501 gaacttcaac gggcgcgatc acatcgagaa catccagaat ttgtttgata accaactggc
    76561 aagcaaccac atcagaaacc agttcctgat cggcattcag cggctgggac atcgtaaact
    76621 cctgatagca ttttgaaagc cgttatagta gcggcttcac atcttcagcg atagtcacat
    76681 ccaccgtcat cagaacacaa aaaaacctgc cggagcaggt tttttgttat cggaacatat
    76741 tgcctggcgg tacgtctttg aacgtcttgc aatagttatt gaacatactt ttcaggatct
    76801 tgcgcagttt catcgcggta ctccgaccat ttgttataca ggtgttattg tctttgcgtt
    76861 tataatatga caaccatcac aaaaatcaat ctttatgtga tgtaaatcac ataaataccc
    76921 ctttaatgtt ataaaaatga taatcaaaaa gcagcccccc catttctgac acatacagat
    76981 ggcaagaaat agcgcctgcc aggcgtcttt tccggccatt gtcgcagcgc tgtaacgcgt
    77041 aaaatactgc tttcttttac tcttctggct ggaccatgag acttctgatt ctgactcttt
    77101 ccttaataac gctgacagga tgtacggtta ctcgccaggc ccatgtgagc gaagttgacg
    77161 cggcaacagg tattgttcgg ttggtttatg atcaggcttt tttgcagcat gcccgtaccg
    77221 ctcgttatgt cagtcgcggg atcgccgatc gcgcatgcca gcaggcaggc tatacccacg
    77281 ctatcccctt tggtcagcca gtaggcaact gtagcctctt tgcgggttcc ttatgtttga
    77341 atacggaatt cactttgtca tatcagtgcc atcattccgc cttccctgtc tttctctaaa
    77401 cgttttaatc agcgaggggg atcttcgctg attaaataaa tagcggtaat gcgttttatt
    77461 cccattcgta tttttaataa atgatgttta tattttacct tttgcaaata ataaaataac
    77521 aaattatagt gacgccacag caacaaatat aacctttgtg gagcactatc atgctgaaac
    77581 cagaaatgat taaaaaactt aatgagcaga tgaacctgga actgtactct tcactgcttt
    77641 atcagcaaat gagcgcctgg tgcagctatc ataccttcga aggtgctgcc gcgttcctgc
    77701 gccgtcacgc ccaggaagag atgacgcata tgcagcgtct gtttgattac ctgactgata
    77761 ccggcaactt accgcgtatt aataccgttg aatctccgtt tgctgaatat tcctcacttg
    77821 atgaattatt ccaggaaacc tataaacacg aacaattaat cacgcagaaa attaacgaac
    77881 tggctcatgc tgcaatgacc aatcaggact acccaacatt taatttcctg caatggtatg
    77941 tttctgagca gcatgaagaa gagaaactgt tcaaatcgat tattgataaa ttaagcctgg
    78001 caggcaaaag tggcgaaggt ctgtatttta tcgacaaaga actctctacc ctcgacacac
    78061 aaaactaatg ctctccggcg gcaggttttc tgccgccgtt tcaaatcagt gacggcaaat
    78121 cttactctga tcggttgaaa aaccttcttc tgaaggtata aatttgcctt ttgctgccag
    78181 aaacgccacc aactctcccg cagtcatccc ttctgccgag caggtgtgaa aacgtgcctg
    78241 ttcaccaaaa cgcgctttaa ttgctgcttc cagactggca tgcgtatatt gctcgcctga
    78301 ttcaatcatc atatttaaca cttcgtgacc gtgaatagag tccatcgttc ctcctcaaaa
    78361 aaagcctagc gtagcgattg ccgcttatga agactttgcg ccagcgcaga actgaacgct
    78421 ttttattgta catttatatt tacaacatat gtaacgtcgg tttgacgaat cagccgttat
    78481 gccttaacct gcgccgcaga tatcactcat aaagatcgtc aggacagaag aaagcgtgaa
    78541 aaacagaact ctgggaagtg tttttatcgt ggcgggaacc acaattggcg ctggtatgct
    78601 ggcaatgccg ctggctgcgg caggtgttgg ttttagcgtc acgttaatct tgttaattgg
    78661 gctttgggcg ttgatgtgct acacggcgct attactgctg gaggtgtacc agcatgtccc
    78721 ggcagatacc ggtctgggca cgctggcaaa acgctatctg ggacgctacg gtcagtggct
    78781 gacgggcttc agtatgatgt tcttaatgta tgctctgact gcggcataca tcagcggtgc
    78841 cggtgaattg ttggcctcca gcatcagcga ctggacaggt atttcaatgt cggcaaccgc
    78901 tggcgtgctg ttgttcactt ttgttgccgg tggcgtggtt tgtgtcggaa cttcgctggt
    78961 cgatttattt aaccgttttc tgttcagcgc caaaattatt tttctggtgg tgatgctggt
    79021 attgctgcta ccgcatattc acaaagtgaa tcttttaacc ctgccgttgc aacagggact
    79081 ggcactgtct gcaatcccgg tgatttttac gtcgtttggt tttcacggta gcgtgccgag
    79141 tattgtcagc tatatggatg gcaacattcg taagctacgc tgggtgttta taatcggtag
    79201 tgcgatcccc ctggtggcat atattttctg gcagctggcg acccttggca gcattgattc
    79261 aacaaccttt atgggattgc tggctaatca tgccggatta aacgggctgt tacaggcgtt
    79321 acgcgaaatg gtggcctctc cgcatgttga gctggcagtg catttatttg ctgatttagc
    79381 cctcgccacg tccttcctcg gcgtagcgtt aggcttattt gattatctgg cagatttgtt
    79441 tcagcgttca aataccgttg gtggacgatt gcaaactggt gcaattacct ttctgccgcc
    79501 actggcgttt gcactgtttt atccacgagg atttgtgatg gcgctgggtt acgccggtgt
    79561 ggcactggcg gtactggcat tgattatccc ttcgctgttg acctggcaaa gcaggaagca
    79621 caatcctcag gcgggttacc gggtgaaagg tggtcgtccg gcgctggtgg tggtgtttct
    79681 ctgtggtatt gctgtgattg gcgtgcaatt tttgattgcg gcaggcttgt taccagaagt
    79741 ggggtgatta gatagcct
//
LOCUS       DAZFFE010000007        72467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFFE010000007 DAZFFE010000000
VERSION     DAZFFE010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 72467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.007"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(36..112)
                     /locus_tag="ACHJ69_000602"
     tRNA            complement(36..112)
                     /locus_tag="ACHJ69_000602"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(76..78),aa:Arg,seq:acg)
     gene            complement(177..253)
                     /locus_tag="ACHJ69_000603"
     tRNA            complement(177..253)
                     /locus_tag="ACHJ69_000603"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(217..219),aa:Arg,seq:acg)
     gene            complement(257..349)
                     /locus_tag="ACHJ69_000604"
     tRNA            complement(257..349)
                     /locus_tag="ACHJ69_000604"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(313..315),aa:Ser,seq:gct)
     gene            complement(664..849)
                     /gene="csrA"
                     /locus_tag="ACHJ69_000605"
     CDS             complement(664..849)
                     /gene="csrA"
                     /locus_tag="ACHJ69_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO5013663.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1084..3714)
                     /gene="alaS"
                     /locus_tag="ACHJ69_000606"
     CDS             complement(1084..3714)
                     /gene="alaS"
                     /locus_tag="ACHJ69_000606"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO5013664.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3843..4343)
                     /gene="recX"
                     /locus_tag="ACHJ69_000607"
     CDS             complement(3843..4343)
                     /gene="recX"
                     /locus_tag="ACHJ69_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO5013665.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4412..5473)
                     /gene="recA"
                     /locus_tag="ACHJ69_000608"
     CDS             complement(4412..5473)
                     /gene="recA"
                     /locus_tag="ACHJ69_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO5013666.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5553..6050)
                     /gene="pncC"
                     /locus_tag="ACHJ69_000609"
     CDS             complement(5553..6050)
                     /gene="pncC"
                     /locus_tag="ACHJ69_000609"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO5013667.1"
                     /translation="MTDSELMQLSEQVGQALKVRGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6195..7280)
                     /gene="mltB"
                     /locus_tag="ACHJ69_000610"
     CDS             complement(6195..7280)
                     /gene="mltB"
                     /locus_tag="ACHJ69_000610"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO5013668.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7537..8100
                     /gene="srlA"
                     /locus_tag="ACHJ69_000611"
                     /gene_synonym="gutA"
     CDS             7537..8100
                     /gene="srlA"
                     /locus_tag="ACHJ69_000611"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO5013669.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVRLAGATS"
     gene            8097..9044
                     /gene="srlE"
                     /locus_tag="ACHJ69_000612"
                     /gene_synonym="gutE"
     CDS             8097..9044
                     /gene="srlE"
                     /locus_tag="ACHJ69_000612"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO5013670.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGLGNIPPHLALPALFAINAQAACDFIPVGLSL
                     AEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9055..9426
                     /gene="srlB"
                     /locus_tag="ACHJ69_000613"
                     /gene_synonym="gutB"
     CDS             9055..9426
                     /gene="srlB"
                     /locus_tag="ACHJ69_000613"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO5013671.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGRHRYPVTAVGSVAEDNLRELGHVTLRFDGLSEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9430..10209
                     /gene="srlD"
                     /locus_tag="ACHJ69_000614"
     CDS             9430..10209
                     /gene="srlD"
                     /locus_tag="ACHJ69_000614"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO5013672.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPEQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10315..10674
                     /gene="gutM"
                     /locus_tag="ACHJ69_000615"
     CDS             10315..10674
                     /gene="gutM"
                     /locus_tag="ACHJ69_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO5013673.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHVGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10741..11514
                     /gene="srlR"
                     /locus_tag="ACHJ69_000616"
                     /gene_synonym="gutR"
     CDS             10741..11514
                     /gene="srlR"
                     /locus_tag="ACHJ69_000616"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO5013674.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11507..12472
                     /gene="gutQ"
                     /locus_tag="ACHJ69_000617"
     CDS             11507..12472
                     /gene="gutQ"
                     /locus_tag="ACHJ69_000617"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO5013675.1"
                     /translation="MSEALLNAGRQTLMLELQEASHLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDDQRLVKGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12469..13983)
                     /gene="norR"
                     /locus_tag="ACHJ69_000618"
     CDS             complement(12469..13983)
                     /gene="norR"
                     /locus_tag="ACHJ69_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO5013676.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGEAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYNFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14170..15609
                     /gene="norV"
                     /locus_tag="ACHJ69_000619"
     CDS             14170..15609
                     /gene="norV"
                     /locus_tag="ACHJ69_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO5013677.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIVYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVEEETSAATTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15606..16739
                     /gene="norW"
                     /locus_tag="ACHJ69_000620"
     CDS             15606..16739
                     /gene="norW"
                     /locus_tag="ACHJ69_000620"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO5013678.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRCIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INSQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WHIYTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLSM"
     gene            complement(16961..19213)
                     /gene="hypF"
                     /locus_tag="ACHJ69_000621"
     CDS             complement(16961..19213)
                     /gene="hypF"
                     /locus_tag="ACHJ69_000621"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO5013679.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGVMNTQI
                     VPDAATCPACLAEMNTPGERRWRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYCDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPTAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHLWPLDGGDVIALTLDGIGMGE
                     NCALWGGECLRVNYRECQHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAAPCHRVTHPVTMPLVDNQLDLATFWQQWLSWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIVAA
                     RWLAGEVQNG"
     gene            complement(19366..19893)
                     /gene="hydN"
                     /locus_tag="ACHJ69_000622"
     CDS             complement(19366..19893)
                     /gene="hydN"
                     /locus_tag="ACHJ69_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO5013680.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            20278..20706
                     /locus_tag="ACHJ69_000623"
     CDS             20278..20706
                     /locus_tag="ACHJ69_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013681.1"
                     /translation="MGSWGMEALESDEGLDLINWVEEQLQDDSTFDAESIVQRLSQHE
                     DLFGFQGDEEFLYDNNVIGLVELIIQKAAGEKITSSKQIDQLDSYRLTSIFSKKLQGR
                     LQTIDDTHEWIMLFEGRAREKAKAYLIEITDKLRVVKTTA"
     gene            complement(20798..21217)
                     /locus_tag="ACHJ69_000624"
     CDS             complement(20798..21217)
                     /locus_tag="ACHJ69_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5013682.1"
                     /translation="MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPVGAALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(21214..21525)
                     /locus_tag="ACHJ69_000625"
     CDS             complement(21214..21525)
                     /locus_tag="ACHJ69_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO5013683.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(21688..22443)
                     /locus_tag="ACHJ69_000626"
     CDS             complement(21688..22443)
                     /locus_tag="ACHJ69_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013684.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     DQNWHFRKVGVAGVTLEKMPNEDDYWRLTAKDRAGKSWFVPVGVLQNMAGNAQFYRAD
                     LDRNGIQDLVIWRGVSGNGLAPSSFLILMTFNQQGRPCVFQVDGFYTANETGVDDLLD
                     LQGNGHTQLLDMQFDSGYWITSLYQVKEAKWQRVHGWFGKLSYPALTRFTYTSNRKLV
                     IKPVAGRNPQTKDLALTQRCLIKGDVFDGVNQN"
     gene            complement(22486..22956)
                     /gene="hycI"
                     /locus_tag="ACHJ69_000627"
     CDS             complement(22486..22956)
                     /gene="hycI"
                     /locus_tag="ACHJ69_000627"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO5013685.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPADIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQQIKDAVETVYQRLEGWEGNGGFAQLAAEEE"
     gene            complement(22949..23359)
                     /gene="hycH"
                     /locus_tag="ACHJ69_000628"
     CDS             complement(22949..23359)
                     /gene="hycH"
                     /locus_tag="ACHJ69_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO5013686.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(23356..24123)
                     /gene="hycG"
                     /locus_tag="ACHJ69_000629"
     CDS             complement(23356..24123)
                     /gene="hycG"
                     /locus_tag="ACHJ69_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO5013687.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIDSMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(24123..24665)
                     /gene="hycF"
                     /locus_tag="ACHJ69_000630"
     CDS             complement(24123..24665)
                     /gene="hycF"
                     /locus_tag="ACHJ69_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO5013688.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWQFDLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(24675..26384)
                     /gene="hycE"
                     /locus_tag="ACHJ69_000631"
     CDS             complement(24675..26384)
                     /gene="hycE"
                     /locus_tag="ACHJ69_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO5013689.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(26402..27325)
                     /gene="hycD"
                     /locus_tag="ACHJ69_000632"
     CDS             complement(26402..27325)
                     /gene="hycD"
                     /locus_tag="ACHJ69_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO5013690.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(27328..29154)
                     /gene="hycC"
                     /locus_tag="ACHJ69_000633"
     CDS             complement(27328..29154)
                     /gene="hycC"
                     /locus_tag="ACHJ69_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO5013691.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLINHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(29151..29762)
                     /gene="hycB"
                     /locus_tag="ACHJ69_000634"
     CDS             complement(29151..29762)
                     /gene="hycB"
                     /locus_tag="ACHJ69_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO5013692.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCATVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(29887..30348)
                     /gene="hycA"
                     /locus_tag="ACHJ69_000635"
     CDS             complement(29887..30348)
                     /gene="hycA"
                     /locus_tag="ACHJ69_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO5013693.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            30560..30910
                     /gene="hypA"
                     /locus_tag="ACHJ69_000636"
     CDS             30560..30910
                     /gene="hypA"
                     /locus_tag="ACHJ69_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO5013694.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            30914..31786
                     /gene="hypB"
                     /locus_tag="ACHJ69_000637"
     CDS             30914..31786
                     /gene="hypB"
                     /locus_tag="ACHJ69_000637"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO5013695.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            31777..32049
                     /gene="hypC"
                     /locus_tag="ACHJ69_000638"
     CDS             31777..32049
                     /gene="hypC"
                     /locus_tag="ACHJ69_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO5013696.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            32049..33170
                     /gene="hypD"
                     /locus_tag="ACHJ69_000639"
     CDS             32049..33170
                     /gene="hypD"
                     /locus_tag="ACHJ69_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO5013697.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            33167..34177
                     /gene="hypE"
                     /locus_tag="ACHJ69_000640"
     CDS             33167..34177
                     /gene="hypE"
                     /locus_tag="ACHJ69_000640"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO5013698.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHRG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            34251..36329
                     /gene="flhA"
                     /locus_tag="ACHJ69_000641"
     CDS             34251..36329
                     /gene="flhA"
                     /locus_tag="ACHJ69_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO5013699.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFNTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            36374..36802
                     /locus_tag="ACHJ69_000642"
     CDS             36374..36802
                     /locus_tag="ACHJ69_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HFO5013700.1"
                     /translation="MAVSARNQLTGTVSAVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(36841..37185)
                     /locus_tag="ACHJ69_000643"
     CDS             complement(36841..37185)
                     /locus_tag="ACHJ69_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO5013701.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            37471..40032
                     /gene="mutS"
                     /locus_tag="ACHJ69_000644"
     CDS             37471..40032
                     /gene="mutS"
                     /locus_tag="ACHJ69_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO5013702.1"
                     /translation="MSTIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQSESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMERQQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLENVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            40138..40794
                     /gene="pphB"
                     /locus_tag="ACHJ69_000645"
     CDS             40138..40794
                     /gene="pphB"
                     /locus_tag="ACHJ69_000645"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO5013703.1"
                     /translation="MPSTRYQKINAHHYRHIWTVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALDAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDTIKYVIAHADYPGKEYQFGKEIAESELL
                     WPVDRVQKSLNSELQKINGADFFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(40845..41612)
                     /gene="ygbI"
                     /locus_tag="ACHJ69_000646"
     CDS             complement(40845..41612)
                     /gene="ygbI"
                     /locus_tag="ACHJ69_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFO5013704.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGRCIYLDAGT
                     TTLAIAQHLIHMEPLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKLDLSLLVAKNE"
     gene            41808..42716
                     /gene="ltnD"
                     /locus_tag="ACHJ69_000647"
     CDS             41808..42716
                     /gene="ltnD"
                     /locus_tag="ACHJ69_000647"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFO5013705.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNATQVKQVLFGEKGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFGLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGSEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            42713..43975
                     /gene="otnK"
                     /locus_tag="ACHJ69_000648"
     CDS             42713..43975
                     /gene="otnK"
                     /locus_tag="ACHJ69_000648"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFO5013706.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVLEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVES
                     QSTGRCGVVSAHVFEQGVKAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQARKAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSIETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            43972..44610
                     /locus_tag="ACHJ69_000649"
     CDS             43972..44610
                     /locus_tag="ACHJ69_000649"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO5013707.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNIPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            44615..45391
                     /locus_tag="ACHJ69_000650"
     CDS             44615..45391
                     /locus_tag="ACHJ69_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO5013708.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSPQQ
                     IQQQLVQNHLTLALFNTAPGDINAGEWGLSALPGREHEARADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            45480..46844
                     /locus_tag="ACHJ69_000651"
     CDS             45480..46844
                     /locus_tag="ACHJ69_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFO5013709.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WLVI"
     gene            complement(46987..48030)
                     /locus_tag="ACHJ69_000652"
     CDS             complement(46987..48030)
                     /locus_tag="ACHJ69_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFO5013710.1"
                     /translation="MQGIKIYTILASSLISGSIFAQTEISTANSSTVNASYVEPSVKK
                     IISPSDKLNNLFERNMSQPYILQKIGERTYYVQRYFYSTTFYVGDKGVLLFDAPEGRG
                     KYLLQAIRDVTPLPVTALVYSHYHVDHIGDSPFWNDEAKKEGVNLRIIASKATAEKMQ
                     FMNSRLPVATQVLSKKDDQFKFEKQTIELHRFVKAGHTDDHSVWLLKQEKVAHSPDLL
                     NPDQLPMMGFAVSDTLVYHDSNLRQVEMLDWKYFIGGHGNIGSHDDFKFQRQFLNDLR
                     DTTIKVRKEESFGKFMNKTANNHADFARAQREAIIKKVTEVLRPKYGHMYGYDASMPA
                     NIEMAIRLVGSYY"
     gene            48255..48806
                     /locus_tag="ACHJ69_000653"
     CDS             48255..48806
                     /locus_tag="ACHJ69_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFO5013711.1"
                     /translation="MQNIAVVYYSGYGHTKLVAEKVAQGAHADLISIDNEGNITEQEW
                     EKLDAADAIIFGAPTYMGGAPWQFKKFADASSKAWFTRKWQDKVFGGFTNSASLNGDK
                     QVTLIYLQTLASQHGGLWGSLGQAPANVLASTREDVNNLGGSVGLLVQSPADAGADQI
                     PTGDLDTAVKYGERVATITARLK"
     gene            complement(48977..49969)
                     /gene="rpoS"
                     /locus_tag="ACHJ69_000654"
     CDS             complement(48977..49969)
                     /gene="rpoS"
                     /locus_tag="ACHJ69_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO5013712.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEVGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(50032..51126)
                     /gene="nlpD"
                     /locus_tag="ACHJ69_000655"
     CDS             complement(50032..51126)
                     /gene="nlpD"
                     /locus_tag="ACHJ69_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO5013713.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(51311..51937)
                     /gene="pcm"
                     /locus_tag="ACHJ69_000656"
     CDS             complement(51311..51937)
                     /gene="pcm"
                     /locus_tag="ACHJ69_000656"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO5013714.1"
                     /translation="MVSRRVQALLDQLRAQGIQDELVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(51931..52692)
                     /gene="surE"
                     /locus_tag="ACHJ69_000657"
     CDS             complement(51931..52692)
                     /gene="surE"
                     /locus_tag="ACHJ69_000657"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO5013715.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPHPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(52673..53722)
                     /gene="truD"
                     /locus_tag="ACHJ69_000658"
     CDS             complement(52673..53722)
                     /gene="truD"
                     /locus_tag="ACHJ69_000658"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO5013716.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKVLMITAALPGSGEWGTQREALAFEQ
                     AAVAEETELQTLLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(53719..54198)
                     /gene="ispF"
                     /locus_tag="ACHJ69_000659"
     CDS             complement(53719..54198)
                     /gene="ispF"
                     /locus_tag="ACHJ69_000659"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO5013717.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYALGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(54198..54908)
                     /gene="ispD"
                     /locus_tag="ACHJ69_000660"
     CDS             complement(54198..54908)
                     /gene="ispD"
                     /locus_tag="ACHJ69_000660"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO5013718.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPRITVVDGGEERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(54927..55238)
                     /gene="ftsB"
                     /locus_tag="ACHJ69_000661"
     CDS             complement(54927..55238)
                     /gene="ftsB"
                     /locus_tag="ACHJ69_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO5013719.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNNDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(55431..55754)
                     /locus_tag="ACHJ69_000662"
     CDS             complement(55431..55754)
                     /locus_tag="ACHJ69_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO5013720.1"
                     /translation="MRNSHNITLINNDSLTEVEDTTWSLPGAVVGFLSWLCALAIPML
                     IYGSNTLLFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLATVGIMFGALF
                     MWLLG"
     gene            complement(55804..56409)
                     /gene="cysC"
                     /locus_tag="ACHJ69_000663"
     CDS             complement(55804..56409)
                     /gene="cysC"
                     /locus_tag="ACHJ69_000663"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO5013721.1"
                     /translation="MALHDENVVWHSHPVTPQQREQHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(56409..57836)
                     /gene="cysN"
                     /locus_tag="ACHJ69_000664"
     CDS             complement(56409..57836)
                     /gene="cysN"
                     /locus_tag="ACHJ69_000664"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO5013722.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIHYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(57838..58746)
                     /gene="cysD"
                     /locus_tag="ACHJ69_000665"
     CDS             complement(57838..58746)
                     /gene="cysD"
                     /locus_tag="ACHJ69_000665"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO5013723.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            58998..60050
                     /gene="iap"
                     /locus_tag="ACHJ69_000666"
     CDS             58998..60050
                     /gene="iap"
                     /locus_tag="ACHJ69_000666"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO5013724.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAECLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAILMGLK"
     gene            complement(60161..60313)
                     /locus_tag="ACHJ69_000667"
     CDS             complement(60161..60313)
                     /locus_tag="ACHJ69_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5013725.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(60576..61310)
                     /gene="cysH"
                     /locus_tag="ACHJ69_000668"
     CDS             complement(60576..61310)
                     /gene="cysH"
                     /locus_tag="ACHJ69_000668"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO5013726.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGIAEEETRFFGLKRECGLHEG"
     gene            complement(61384..63096)
                     /gene="cysI"
                     /locus_tag="ACHJ69_000669"
     CDS             complement(61384..63096)
                     /gene="cysI"
                     /locus_tag="ACHJ69_000669"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO5013727.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREVGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(63096..64895)
                     /gene="cysJ"
                     /locus_tag="ACHJ69_000670"
     CDS             complement(63096..64895)
                     /gene="cysJ"
                     /locus_tag="ACHJ69_000670"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO5013728.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGTVNEIHTSPYSKDAPLAASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPTN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            65214..65576
                     /gene="queD"
                     /locus_tag="ACHJ69_000671"
     CDS             65214..65576
                     /gene="queD"
                     /locus_tag="ACHJ69_000671"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO5013729.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPQGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            65654..66925
                     /locus_tag="ACHJ69_000672"
     CDS             65654..66925
                     /locus_tag="ACHJ69_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5013730.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLLPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLTLEPSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLRSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRVWPGLMQDISRDLWDQGNTP
                     VPPLRQLLWRHLRRHGLWHLTGDVIRSLRCL"
     gene            66916..67176
                     /locus_tag="ACHJ69_000673"
     CDS             66916..67176
                     /locus_tag="ACHJ69_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO5013731.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQTAQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            67193..67768
                     /locus_tag="ACHJ69_000674"
     CDS             67193..67768
                     /locus_tag="ACHJ69_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO5013732.1"
                     /translation="MSLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEAYGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVTQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(67916..68776)
                     /locus_tag="ACHJ69_000675"
     CDS             complement(67916..68776)
                     /locus_tag="ACHJ69_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO5013733.1"
                     /translation="MNIAIVTINQEHAAMAGWLAAQDFSGSTLTHWQIEPQPMVAEQV
                     LDALVEQWQRTPAEVVLFPPGAFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGADKNATLPFGMQQLNIVPGALPDWLISVENLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKMGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(68773..69552)
                     /locus_tag="ACHJ69_000676"
     CDS             complement(68773..69552)
                     /locus_tag="ACHJ69_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO5013734.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWLAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLVQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQYQNPLDLIITGCQSSEGQNGQTPFLLAEMLVWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMATGKAEITRETVAAEAPA
                     IQCLQLARPEQRRRAALIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(69530..71014)
                     /locus_tag="ACHJ69_000677"
     CDS             complement(69530..71014)
                     /locus_tag="ACHJ69_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5013735.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFMLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISESPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLASAALLALLITLLRWGTPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGD
                     EVATATHKHIKTLFSSRYWRRTAFNSVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTA
                     SLMLNALLIVGALLGLVLTHLLAHRRFLLGSFLLLAATLVVMACLPSGSSLTLLLFVL
                     FSTTISAVSNLVGILPAESFPTDIRSLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQA
                     TLLLLAAVLLVGFVVTWLWAPETKALPLVAAGNVGGANEHTVSV"
     gene            complement(71036..>72467)
                     /locus_tag="ACHJ69_000678"
     CDS             complement(71036..>72467)
                     /locus_tag="ACHJ69_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO5013736.1"
                     /translation="VDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHGIYTLPIPA
                     AVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDGSAMNQIIN
                     IDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRSIGQFSTLY
                     GAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITEVTVKIFKF
                     TPENNLFYGYVLEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHFADGKCVLI
                     FMAEGNPRMAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQILK
                     TGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPYADDITMLGGHSSHSYQNGTNMYFV
                     YDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGSAW
                     ALLEGLKKQFDPNGIMNTGTIYPIEK"
BASE COUNT        17279 a        19652 c        18469 g        17067 t
ORIGIN      
        1 ttgataccgc cactaaagcc atatcaagta agagatggtg catccgggag gattcgaacc
       61 tccgaccgct cggttcgtag ccgagtactc tatccagctg agctacggat gcatcgggaa
      121 acttacttta ctgcagattt tttgataccg ccactaaagt cgtatcaagt aagagatggt
      181 gcatccggga ggattcgaac ctccgaccgc tcggttcgta gccgagtact ctatccagct
      241 gagctacgga tgcaaatggc ggtgaggcgg ggattcgaac cccggatgca gcttttgacc
      301 gcatactccc ttagcagggg agcgccttca gcctctcggc cacctcacca cacgcctctt
      361 acgagtgctt cgaagaactt gtttatcgct catcgtcgct gcgtggcgca catattactt
      421 tctgagactt ataagtcaaa caatttttcc cacactttta tcgtttgcac acttcacgct
      481 caattagtct gtaaaaacgt caaaaagagt gttttatcaa cagaaaaacg gaggtctgac
      541 agatagcagt aatgcaaaaa atggagactt aagttgaatg aacgggagta aagcgaaaag
      601 acaatggagt gaaagagaaa ttttgagggt gcgtctcacc gataaagatg agacgcggaa
      661 agattagtaa ctggactgct gggatttttc agcctggata cgctggtaga tctcttcacg
      721 gtgaacagaa acttccttcg gggcatttac gccaatacgt acctggttgc cctttacccc
      781 taaaactgtc acggtgacct catccccaat catgagggtc tcaccaactc gacgagtcag
      841 aatcagcatt ctttgctcct tgaaagatta aaagagtcgg gtctctctgt atcccggcat
      901 tatccatcat ataacgccaa aaagtaagca atgacaaaca cattacatct aagcagtcat
      961 ggcattacat tctgttaaac ctaagtttag ccgatataca caacttcaac ctgactttat
     1021 cgttgtcgat agcgttaatg cgaatgccgt gaagcgagtc cacggcattg cctgacgctt
     1081 atattattgc aatttcgcgc tgacccagcc tttcacactg gctaacgctg caggtaaggc
     1141 cgcagcatcc gtaccaccgg cttgcgccat gtcaggacgt ccaccaccct tgccgcccac
     1201 ctgctgagcg accataccaa tcagttcccc tgctttcaca cgatctgtga cgtccttaga
     1261 tacgcctgca atcagagaaa ccttaccttc ggctaccgtt gccagcacga taattgtcga
     1321 ccccagctga ttttttaaat cgtcaaccat ggtacgcaac attttcggct caacaccgct
     1381 aagctcgcta accaacagct taacaccatt aacatcaatt gccttactgg aaagatttgc
     1441 gctctcctgt gcggcagctt gttctttaag ctgttgtaac tctttttcca gctgacgcgt
     1501 acgttccagt actgaacgca ctttatcagc cagattattg ctatcgcctt tcagcagatg
     1561 cgcgacttcg cttaagcgat cgctgtctgc atgaacggtg gcgatagcgc cttctccggt
     1621 caccgcttcg atacgacgaa cgcctgcagc agtacccgat tcagagatga tgcggaacag
     1681 accaatatca ccagtgcggc tggcgtgagt accgccacac agctcggtgg agaaatcgcc
     1741 catgctcagc acgcgtacgc gctcatcata cttctcgcca aacagcgcca tcgcaccttt
     1801 cgctttcgcc gcttcgagat ccatgatgtt ggtttcgatc ggcaagttac ggcgaatctg
     1861 tgcgttcacc aggtcttcga ccgcacgaat ctcttctggt ttcatcgctt cgttgtgtga
     1921 gaagtcgaag cgcagcactt tgtcgttaac cagtgaacct ttctgcgata catgagtacc
     1981 cagaacctgg cgcagcgcag cgtgcatcag gtgcgttgcg gagtgattca gacgaatacg
     2041 ggcgcgacga gcctcatcaa catcagcctg cacagcgtcg cccactttca gagaacccgc
     2101 agcaagttta ccgatgtggc caatcgcctg gccgtatttc tgcgtatctt ccaccgcgaa
     2161 ggagaagtta gcgcctttca gttcgccttt atcaccaacc tgaccgccgg attccgcata
     2221 gaatggcgtt tgatccagca cgaccacagc ttcctggcct gcattgatgg catcaaccgc
     2281 tttaccatca acaaacagcg cggtcacttt gccgttcagt tccagatggt catagccttt
     2341 aaattcagat gcactgtcaa cacggatcat tgcgttgtaa tcggcaccaa agccgctggc
     2401 ttcgcgcgca cgacgacgct gctcttccat tgctgcttca aaaccagctt cgtcaacttt
     2461 gatgttgcgc tcacgacaaa catcagccgt caggtcaacc gggaagccgt aggtgtcgta
     2521 caggcggaaa gcagtttcac catccagcgt atcaccagaa agttttgcca gctcttcatc
     2581 cagcagcgcc agaccgcgct ccagagtacg ggcaaactgc tcttcttcag tcttcagcac
     2641 ctgctcaacc tgcgcctgct ggcgtttcag gtcttcaccc gcagagccca taacgtcgat
     2701 cagcggacca accagtttgt agaagaaggt ttctttcgcg ccgagcatat tgccgtgacg
     2761 cactgcgcga cgaatgatac gacgcagtac ataaccacgg ttttcgttgg acggcattac
     2821 gccatccgcg atcaggaacg cacaagaacg aatgtggtca gcgattacgc gcagcgattt
     2881 attgctcaga tcggtcgcgc cagtgacttt cgctaccgcc tggatcaacg tgcggaacag
     2941 gtcgatgtca tagttagagt taacgtgttg cagcaccgca gcaatacgct ccagacccat
     3001 accggtatct acagacggtt tcggcagcgg ttccatcgtg ccatcggcct ggcggttgaa
     3061 ctgcatgaag acgatgttcc agatctcaat gtagcggtcg ccgtcttctt ccggacttcc
     3121 cggagggccg ccccaaatgt ggtcgccgtg atcgtagaag atttcggtgc acgggccgca
     3181 cggaccagtg tcacccatct gccagaagtt gtcagatgcg tatggcgcac ctttgttatc
     3241 gccgatgcga ataatacgtt cgcgcgggat ccctacttct ttttcccaga tttcgtaggc
     3301 ttcgtcgtcg ctttcataga cggtaaccca cagacgctct ttcggcaggg caaaccattt
     3361 ttcgctggtc agcagttccc atgcaaactg aatggcatcg tgtttgaaat agtcgccgaa
     3421 gctgaagttg cccagcattt cgaagaaggt atggtgacgc gcggtgtaac cgacgttttc
     3481 caggtcgttg tgtttaccac ccgcacgcac gcagcgttgg gaagtggtag cgcgggaata
     3541 attacgcttg tcgagcccaa ggaacacatc cttgaactgg ttcatcccgg cgttggtaaa
     3601 caacagagtt gggtcgttat ggggtaccag ggagctgctg gcaactacct gatgtccctt
     3661 actatggaaa aagtcgagaa acgcctgacg gatctcagcg gtgctcttgc tcataattat
     3721 cctgaaatca agctaacgaa atatcgctac cagccgttgc gtgtcgttaa ccgcccggct
     3781 ggtaactgaa aagtgggaat aagataagtt ttcttgactg ggaagtaaaa tcccgtatgc
     3841 gttcagtcgg caaaatttcg ccaaatatcc tggatatctt ccatcagata gccacgatag
     3901 agcagaaaac gctggatctt aactttttct gaaaagacag ttggcaaagg ttcgccatat
     3961 tttcgcgtcg cctgatcgcg cgccagtgcg caccagtcga tgtcacattc acgcatcgct
     4021 ttttctgtcg cttcgcggga aatacctttc tgattcagtt cctgacgaat acgcgcaggt
     4081 ccataacctt tgcggctacg gctggcgata aagcgcgcaa caaatcggct gtcatcaaga
     4141 tagccatgtt catggcacca ggcaataacg cgctcgtaat cttctgccgt ggcatcaatc
     4201 tcttccgggc catttttgcc cataatcggt gccgcgagtt tacgtcgcag ttcttgctca
     4261 ctgtgatcgc gcaccgccag aatgcgtacc gcacgatcca acaggcgagc atatgccggg
     4321 cgacgggatg ttgattctgt catggcatat ccttacaact taaaaaagca aaagggccgc
     4381 agatgcgacc cttgtgtatc aaacaagacg attaaaaatc ttcgttagtt tctgctacgc
     4441 cttcgctgtc atctacagag aaatccggcg ttgagttcgg gttgctcagc agcaactcac
     4501 gtactttctt ctcgatctct ttcgcggttt ctgggttatc tttcagccag gcagtcgcat
     4561 tcgctttacc ctgaccgatc ttctcacctt tgtagctgta ccacgcgcct gctttctcga
     4621 taagcttctc tttcacgccc aggtcaacca gctcgccgta gaagttgata ccttcgccgt
     4681 agaggatctg gaattcagcc tgtttaaacg gcgcagcgat tttgttcttc accactttca
     4741 cgcgggtttc gctacccacc acgttttcgc cctctttcac cgcgccgata cgacggatgt
     4801 cgagacgaac agaggcgtag aatttcagcg cgttaccacc ggtagtggtt tccgggttac
     4861 caaacatcac accaattttc atacggatct ggttgatgaa gatcagcagc gtgttggact
     4921 gcttcaggtt acccgccagc ttacgcatcg cctggctcat catacgtgcc gcaaggccca
     4981 tgtgagagtc gccgatttcg ccttcgattt ccgctttcgg cgtcagtgcc gccacggagt
     5041 caacgacgat aacgtctact gcgccagaac gtgccagggc gtcacagatt tccagtgcct
     5101 gctcgccggt atccggctgg gagcacagca ggttgtcgat atcgacgccc agtttacgtg
     5161 cgtagattgg gtccagcgcg tgttcagcat cgataaacgc acaggtttta ccttcgcgct
     5221 gcgctgcggc gatcacctgc aacgtcagcg tggttttacc ggaagattct ggtccgtaga
     5281 tttcgacgat acggcccatc ggcagaccac ctgccccaag cgcgatatcc agtgaaagcg
     5341 aaccggtaga gatggtttcc acatccatgg aacggtcttc acccaggcgc atgatggagc
     5401 ctttaccaaa ttgtttctca atctggccca gtgctgccgc caacgctttc tgtttgtttt
     5461 cgtcgatagc catttttact cctgtcatgc cgggtaatac cggatagtca atatgttctg
     5521 ttgaagcaat tatactgtat gctcatacag tatcaagtgt tttgtagaaa ttgttgccac
     5581 aaggtctgca atgcatacgc agtagcctga cgacgcaccg catcacggtc accgctgaag
     5641 cattcccgcc gggtaatgcc ttcaccacgg gcagtggcaa aagcaaacca gacggtgccg
     5701 acaggcttct cttcactgcc gccatccggc ccggcgatac cactaataga cacggcgtaa
     5761 tcagcacgag ccgctttcag tgcgcctatc gccatttcca ccacgacggg ttcactcacc
     5821 gcgccatgct gcgccagcgt ctcttcgcgc acgccgatca tctgcgcttt ggcttcgtta
     5881 ctgtaggtga caaatccgcg ttcaaaccag gcggagctac cggcaatatc ggtaatcact
     5941 ttcgctaccc aaccgccggt acaagactca gcagttgtta cggttgcgcc acggactttc
     6001 agcgcctgcc caacctgttc gcttaactgc atcagttcac tgtcagtcat cacaacctct
     6061 gtcagtcata aatttatgcc ggacaagata gcactttcgt caggaagatg gtgttggcgt
     6121 tgcaatttta cgaggaggat tcagaaaaaa gctgattagc cagagggaag ctcacgcccc
     6181 cctctggcaa atagttactg cacgcgcgcc agcgccacgg cttgtcctaa ctgccagacc
     6241 gccattgcgt aatgggtgct gtggttataa cgggtaatgg tgtagaagtt cggcagaccg
     6301 taccagtact ggtaaccggt accgacatcc agacgcagca ggctggcttg ttgatggttg
     6361 cccagcggct gctgtggcgt taaacctgcc gcggcaagct gcgaaatgct gtacctggtt
     6421 ttgaagccat ttggcaagcc tggcgcctga ccgtttgcca ttaccgcgac ctgatcgcct
     6481 ttcacccagc cgtgcgcttt gaaataattc gccacgctac cgatcgcatc aaccggatcc
     6541 cacaggttga tatgcccgtc gccgctgaaa tctaccgcat attgtttgta agacgacggc
     6601 ataaactgtc cgtagcccat cgccccggca aaggaacctt tcagattgag cggatcgtcc
     6661 tgctcgtcgc gcgccatcag caggaaggtt tccagctcgc cagaaaaata ctccgcgcgg
     6721 cgtgggtagt taaatgacag cgttgccagc gcatcgagga tgcgagtttt ccccatcacg
     6781 cgcccccagc gggtttcaac gccgataatc ccgacgataa tttccggcgg tacaccatac
     6841 acctgccacg cgcgattcaa cgcatcttca tactgattcc agaaaaccac accgttctgc
     6901 acgttgtccg gcgtaataaa ttttttgcga taacgcagcc atgcgccatt cggacctgat
     6961 ggcggtttca ccgatgtggt tggtgcctgg ttatccatca gccgcagtac cgaatccaga
     7021 cgtttcgcct gggagagaat ttcctgcaac tgctgacgat cgaaaccgtg tttgttcacc
     7081 attttgtcga tgaactgctg ggcattcggg ttattggcga agtcaccgcc catctgcatc
     7141 acattgtgct gcggctcaag caggaagccg ccagacggcg ttccggtggt cgtttcagtc
     7201 tcagtaggtt ttggcttgct gctacaggcg gcaagcaaca caaaaagggg aagcaatgtt
     7261 acataacgac gcttgaacat gaggggtcca tttaacagat tcaaccaggg gcaagtatgg
     7321 taaagcatcc cgccccgcac aaggaagcgg tagtcactgc ccgatacgga ctttacataa
     7381 ctcaactcat tctcctcact accttcttta tacaaacttt caaaataaaa tatttatctt
     7441 tcattttgcg atcaaaataa cacttttaaa tctttcaatc tgattagatt agattgccgt
     7501 ttggtaataa aacaataaat actgaaggag agaacaatga tagaaaccat tactcatggc
     7561 gcagagtggt tcatcgggct gttccagaaa ggcggagagg tgtttaccgg gatggtgacc
     7621 ggcattcttc cgctgttaat tagcctgctg gttatcatga acgcattgat taattttatc
     7681 ggtcagcatc gtattgaacg atttgctcaa cgttgcgccg gtaaccctgt ttcccgttat
     7741 ctgctgctac cgtgcattgg tacgtttgtc ttttgtaacc cgatgacctt aagtcttggt
     7801 cgctttatgc cggaaaagta caaacccagc tattacgcgg cggcctctta tagctgccac
     7861 tcaatgaatg gcctcttccc ccatatcaac cctggcgaac tgtttgttta tctcggcatt
     7921 gccagcggtc tgacaacact gaatctgcca cttggcccac tggcggtgag ctatctgctg
     7981 gttggtctgg tcaccaactt cttccgcggc tgggtaaccg atctgaccac cgccattttc
     8041 gagaaaaaga tgggcattca acttgaacaa aaagttcgtc tggcaggagc aacatcatga
     8101 cacgcattcg gatcgaaaaa ggaacgggtg gctggggcgg cccgcttgag ctggaagcca
     8161 cgccgggcaa aaaaatcgtc tatatcaccg ccggtacccg gcctgcgatt gttgacaaac
     8221 tggcacagct tactggctgg caagctatcg acggatttaa agaaggtgaa cccgcggagg
     8281 cggaaattgg tgtcgcggta atcgactgtg gcggcacatt acgctgcggc atctatccga
     8341 aacggcgtat tcccaccatt aatatccact cgacgggcaa gtccggcccg ctggcgcagt
     8401 acattgtgga agatatttat gtctctggcg taaaagaaga aaacatcact gtagtgggtg
     8461 atgcgacacc acaaccctct tccgtgggcc gtgactatga caccagcaag aaaatcaccg
     8521 aacaaagcga tggtttactg gcgaaggtgg gaatgggtat gggttccgcc gttgccgtgt
     8581 tgtttcaatc tggtcgtgac accatcgaca ctgtattaaa aaccattctg ccgtttatgg
     8641 cgttcgtctc ggcgcttatc ggaatcatta tggcttctgg ccttggtgac tggattgccc
     8701 acggtcttgc tccgctggcg agccatccac tgggtctggt catgctggcg ctcatctgct
     8761 ccttcccgct gctttcacct ttcctcggcc caggcgcagt tatcgcacag gttatcggcg
     8821 tattgattgg tctcggcaat attccgccgc atctggcttt acctgcactg tttgccatca
     8881 acgcgcaggc ggcctgcgac ttcatcccgg tcggtttgtc gctggcggaa gctcgtcagg
     8941 acacggttcg cgtcggtgtc ccttctgttc tggtgagccg ctttttaacc ggcgcgccaa
     9001 ctgtactgat cgcctggttt gtctccggtt ttatctatca atagaggctg aaacatgacc
     9061 gttatttatc agaccaccat cacccgtatc ggcgcgagtg ccactgacgc cctcagcgac
     9121 cagatgctca tcacctttcg tgaaggcgcg cctgcggatc tcgaagagta ttgcttcatt
     9181 cattgccacg gcgagttgaa aggtgcactc catcccggtt tgcaattttc tctcgggcgg
     9241 catcgctatc cggtgaccgc cgttggcagc gtggcggaag acaaccttcg cgaactgggt
     9301 catgtcaccc tgcgcttcga tggtttaagc gaagcggaat ttccgggcac tgtccatgtg
     9361 gcaggccctg tacccgacga tatcgcgccg ggatcggttt tgaagtttga atctgttaag
     9421 gagtaaaaaa tgaatcaggt tgccgttgtc atcggtggtg ggcaaacctt aggcgcgttc
     9481 ctgtgccacg gtctggctgc cgaggggtat cgcgtggcgg ttgtcgatat tcagagcgac
     9541 aaagccgcaa atgtggcaca agaaattaac gccgaatatg gtgaaggaac agcgtacggt
     9601 tttggtgctg acgccaccag cgagcaaagc gttctggcgc tttctcgtgg ggtagatgaa
     9661 atctttggtc gcgtggattt gctggtctac agcgccggaa tagcaaaagc agcctttatc
     9721 agcgacttcc agctcggcga ttttgaccgt tcgctacagg tgaatctggt gggttatttc
     9781 ctgtgtgccc gtgaattttc gcgtttgatg atccgcgacg ggattcaggg tcgcattatt
     9841 cagatcaact cgaaatccgg caaagtgggc agcaaacaca actctggcta cagcgcagcg
     9901 aaatttggtg gcgtcgggct gactcaatca ctggcgctgg atctggcgga gtacggcatt
     9961 acggtgcatt cgctgatgct cggtaacctg ctgaaatcgc ctatgttcca gtcactgttg
    10021 ccacaatacg cgaccaagct gggtatcaaa ccggagcaag tcgagcagta ttacatcgat
    10081 aaagtaccgc tcaaacgcgg ctgcgattat caggatgtgc tgaatatgct gctgttctac
    10141 gccagtccta aggcgtcgta ctgcactggt cagtcgatca atgtcaccgg cggtcaggtg
    10201 atgttctgat caacagcgga gatcttttaa ggatctccgc gcgactaata gaatgcctga
    10261 tgcgctacgc ttatcaggcc tacaggactt ccgccactac attacggaga agttatggta
    10321 tccgcactca tcaccgtcgc cgttatcgcc tggtgtgcgc aactggcctt aggcggttgg
    10381 caaatttctc gttttaaccg tgccttcgac acgctatgcc agcaagggag ggttggcgtg
    10441 ggccgttcca gcgggcgctt taaaccgcgg gtcgtggtcg ctattgcgct ggacgatcag
    10501 cagcgcatcg tcgacacctt gtttatgaaa ggactgaccg tcttcgcccg accgcaaaaa
    10561 attcccgcaa ttaccggcat gcatgtgggt gatttacagc ccgatgtgat ctttccccat
    10621 gatccactat cacagaatgc tctatcattg gcgcttaaac tgaaacgtgg ataatttcgt
    10681 tgtgaatgtt acttgcttgc gaagttatca ttttgaaacc taaatcaggt aatcacgccc
    10741 atgaaacctc gtcagcgtca ggccgccatt ctggagtatc tacaaaagca gggtaaatgc
    10801 tcggttgaag aattggcgca atactttgac accacaggca caaccattcg caaagatctg
    10861 gtcattctgg aacatgccgg aaccgtcatt cgtacttatg gcggcgtggt gttgaacaaa
    10921 gaggaatccg atccgcctat cgatcataaa acgcttatca acacccacaa aaaagagctg
    10981 attgcagaag ctgccgttgg ttttattcat gatggcgact caataattct tgatgctggc
    11041 agtaccgtgt tgcagatggt gcccctgctc tcgcgcttta ataacatcac ggtgatgacc
    11101 aacagcctgc acatcgtcaa tgcgctatcc gaactggata acgaacaaac tatactgatg
    11161 ccaggcggaa catttcgcaa aaaatcggcc tcatttcacg ggcaactggc agagaatgcc
    11221 ttcgagcatt tcaccttcga taaattgttt atgggcaccg acggcatcga tctcaatgcg
    11281 ggcgtaacca cctttaacga ggtctatacc gtcagtaagg cgatgtgtaa tgccgcgcgc
    11341 gaagtgattt tgatggctga ctcatcgaag tttggtcgta aaagccccaa cgtggtttgt
    11401 agtcttgaaa gcgtcgataa gctgattacc gacgcaggta tcgatccagc gtttcgtcag
    11461 gcgctggaag aaaaagggat cgacgtgatc ataaccggag agagcaatga gtgaagcact
    11521 actgaacgcg ggacgtcaga cgttaatgct ggagttgcag gaagcaagcc atttaccgga
    11581 acgtctgggc gatgatttcg ttcgcgccgc caatatcatc ctgcactgcg aaggcaaagt
    11641 ggtggtttcg ggaattggca aatcgggcca cattggtaag aaaatcgccg caacgcttgc
    11701 cagcaccggc acaccggctt tttttgtcca tccggcagaa gcactgcacg gcgatctggg
    11761 gatgattgaa agccgcgatg tgatgctgtt tatctcttat tccggtggcg cgaaggaact
    11821 ggatctcatt attccgcgtc tggaagacaa atctatcgct ctgctggcaa tgaccggaaa
    11881 accgacgtca ccgctgggcc tggcggcgaa agcggtgctg gatatctccg tagaacgcga
    11941 agcctgcccg atgcaccttg cgccgacctc cagcaccgtg aataccctga tgatgggcga
    12001 cgcgctggcg atggcggtca tgcaggcgcg cggatttaat gaagaagatt ttgcccgctc
    12061 ccacccagcc ggggcactgg gcgctcgctt gctgaacaaa gtgcatcatc ttatgcgtcg
    12121 tgacgatgcc atcccgcagg tggcgttaac cgccagcgtg atggacgcga tgctggaact
    12181 tagccgcacc ggtttaggtc tggtggcagt gtgcgatgat caacgactgg tgaaaggcgt
    12241 ctttaccgac ggtgatttac gtcgctggct ggttggcggc ggcgcactca ccacgccagt
    12301 caatgaagcg atgacggtcg gcggcaccac gttgcaatcg caaagccgtg ccatcgacgc
    12361 caaagaaatc ctgatgaaac gcaaaatcac cgccgcaccg gtagtggatg aaaacggcaa
    12421 actcaccggc gcaattaacc tgcaggattt ctatcaggcc gggattattt aatccttcag
    12481 ccccagacgt ttcgccagcc tatgcaggtt ggcgacgtcg gtttccagca tccgcgcaca
    12541 ggcggcccag ttgtgatgat tttgcgccag tgcctgacga atggtttcac gctggaacgc
    12601 ttctgtcgct tcacgcaggt tttgcttaac aacgggcacc gccgccactt ctggcggcgg
    12661 caacgtcacc tcaggaaaag caaaatgttg cgcctcaaga atcacttcat cgccgctgcg
    12721 ggttgctctc gccagcacta ccgcccgatg aatagcatgt tccagttcac gaacgttccc
    12781 cggaaaattg tagtgttgca gtaaatttcg cgctccggca cttaatacca ctcgggagag
    12841 ccccagccgc aaacgacact gctcgcagaa ataccccgcc agcagaatga catcatcgcc
    12901 ccgctcacgc agcggcggca ctgaaagtgg aaacacgctc aggcggtgga acaagtcagc
    12961 gcgaaatcgc cctgccagta cctcttcgcg taagtcacgg ttagtcgccg ccagcacgcg
    13021 cacatcgacc cgcaaactgc ggtcatcgcc aacgcgttga atatcgccat actgcaacac
    13081 cctcagcagc ttggcctgca atgccaacga caactcgccg atctcatcga gaaacagcgt
    13141 gccgttatcc gccatttcaa acttcccgct gcggttactg atagcaccag taaacgcccc
    13201 tttcacatgc ccgaacaact cactttccgc cacactttcc ggcagtgcgg cacagttgag
    13261 atagaccagc ggattcaccg cccgtggcga ggcttcatga atcgctttcg ccaccagctc
    13321 cttaccggtt cccgtctcac cgctaattaa aacgttgaga tcggatgccg ccacaatctc
    13381 aatctctttt ttcaattgcg tcatgcctgg cgacagaccg atcatctgcg tctgtttcac
    13441 cgcttcaaac ggcgcggctt cgcctggcag catattctgg ctttccagtt gttcaatcag
    13501 caatgcattg cttaacgctc ccgccgccag tgcggcaatc agccgtagct cttcgtcgct
    13561 gaaaacatcg aactgatcgg gctgcatccc gtcgagcgtc aaggcaccga tcaggttttg
    13621 cccggcaaac aacggcaaac caacgcaggc gtgaaccttc agactctcct gccctggaat
    13681 caaaccgtca taaggatcgg gcaattcgct gtctgcggga aagcgcacca cgtccccggc
    13741 gcgggcaatc gcttccagcc gtggatgacc ttccagcgca aagcgtctac cgagtacatc
    13801 cttcgccagc ccgtcgatgg caagcggaat aaactgccgc gagtcgtaac gtagcaacgc
    13861 agacgcatcg cactccagca cctgacgcag cgtggtgatc aggcgctgaa aacgatcctg
    13921 atgaccaatc ccacgctgca attcgatggc gatattcgcc agcacatcaa cggaaaaact
    13981 catctttgcc tcactgtcaa tttgactata gatattgtca tatcgaccat tttattgata
    14041 gtcattttga ctactcatta atgagcacaa ttttatttat agagtaaaaa caattagata
    14101 aaaaactggc acgcaatctg caattagcaa gacatctttt tagaacacgc tgaataaatt
    14161 gaggttgcta tgtctattgt ggtgaaaaat aacattcatt gggttggtca acgtgactgg
    14221 gaagtgcgtg attttcacgg tacagaatat aaaacgctgc gcggcagcag ctacaacagc
    14281 tacctcatcc gcgaagaaaa aaacgtgctg atcgacaccg tcgaccataa attcagccgc
    14341 gaatttgtgc agaacctgcg taatgaaatc gatctggcgg atatcgatta catcgtaatt
    14401 aaccatgctg aagaggacca cgccggggcg ctgaccgaac tgatggcaca aattcccgat
    14461 acgccgatct actgtactgc caacgctatc gactcgataa atggtcatca ccatcatccg
    14521 gagtggaatt ttaatgtagt gaaaactggc gacacgctgg atatcggcaa cggcaaacaa
    14581 ctcatttttg tcgaaacgcc aatgctgcac tggccggaca gcatgatgac ttacctgaca
    14641 ggcgacgcgg tgctgttcag taacgatgct ttcggtcagc actactgcga cgagcatctg
    14701 ttcaacgatg aagtggatca gactgagctt ttcgagcagt gccagcgtta ctacgccaat
    14761 atcctgacgc cgttcagccg cctggtaacg ccgaaaatta ccgagatcct gggctttaac
    14821 ttgccagtcg atatgatagc gacctctcac ggcgtggtat ggcgcgataa cccgacgcaa
    14881 attgtcgagc tgtacctgaa atgggcggcg gattatcagg aagacagaat caccattgtc
    14941 tacgacacca tgtcgaataa cacccgcatg atggctgacg ctatcgccca ggggattgcg
    15001 gaaaccgacc cacgcgtggc ggtgaaaatt ttcaacgtcg cccgaagcga taaaaacgaa
    15061 atcctgacca atgtcttccg ctcaaaaggc gtactggtcg gcacttctac gatgaataat
    15121 gtgatgatgc cgaaaatcgc cgggctggtg gaggagatga ccggattacg cttccgtaac
    15181 aaacgcgcca gcgctttcgg ctctcacggc tggagcggcg gtgcggtgga tcgtctttcc
    15241 acgcgcctgc aggatgcggg tttcgaaatg tcgcttagcc tgaaagccaa atggcgacca
    15301 gaccaggacg ctctggagtt atgtcgtgaa cacggtcgcg aaatcgcccg tcagtgggcg
    15361 ctcgcgccgc tgccacagag cacggtgaat acggtggttg aagaagaaac ctctgccgcc
    15421 acgacggctg acctcggccc acggatgcaa tgcagcgtct gccagtggat ttacgatccg
    15481 gcaaaaggcg agccaatgca ggacgttgcg ccaggaacgc cgtggagtga agtcccggat
    15541 aacttcctgt gcccagaatg ctctctcggc aaagacgtct ttgacgaact ggcatcggag
    15601 gcaaaatgag caacggcatt gtgatcattg gttcgggctt cgccgcccgc caactggtga
    15661 aaaatattcg caaacaggac gccagtattc cattaaccct gattgccgcc gacagcatgg
    15721 atgagtacaa caaacctgac ctcagccatg ttatcagtca ggggcaacgt gccgacgacc
    15781 ttacccgcca gacggcaggt gaatttgccg agcagtttaa tctgcgcctg tttccgcaca
    15841 cctgggtaac ggatatcgat gccgaagccc atgtggtgaa aagtcagaat aatcagtggc
    15901 aatacgacaa gttagtgctg gcaaccggtg ccagcgcctt tgtcccgcca gtgcccgggc
    15961 gtgagttaat gctgacgtta aatagtcagc aagagtatcg cgcctgtgaa acgcaactgc
    16021 gggatgcccg acgcgtgttg attgtcggcg gtggtttgat tggtagtgaa ctggcgatgg
    16081 atttttgtcg ggcaggcaaa gcggtcacgc taatcgacaa cgctgccagt attctggcgt
    16141 cgttaatgcc accggaagtc agcagccgct tgcagcatcg gttgacggag atgggcgttc
    16201 atctgctgtt gaaatctcag ttgcagggac tagaaaaaac agattctggc attctggcaa
    16261 cgctggaccg ccagcgctgc atcgaagtgg atgcggtaat tgccgccacc ggactgcgcc
    16321 cggaaaccgc cctggcacga cgcgccgggc tgacgattaa tcgcggcgtt tgcgtcgata
    16381 gttatctgca aaccagtaat gccgatattt atgcgctggg cgattgcgcg gaaattaaca
    16441 gtcaggtatt gccgttcctc cagccgattc aacttagcgc gatggtgctg gcaaaaaatc
    16501 ttctcggcaa taacacgccg ctgaaactcc cggcaatgct ggtgaaaatc aaaacgccgg
    16561 aattaccgct gcatctggca ggcgaaaccc agcgtcagga tttacgctgg cacatttata
    16621 ccgaacgcca gggaatggtg gcgcgcggcg ttgacgatgc tgaccagctt cgcgcctttg
    16681 tggtcagtga ggatcggatg aaagaggcat ttggattgtt gaaaacgttg tcgatgtagg
    16741 cgagctacag tgccccaaat gtcggatgcg gcgctgacgc gtcttatccg acctacaggg
    16801 aacgcatgtg ttgatcacat aatacgttta tgtcgcattg cactgtccca aaatgtcgga
    16861 tgcgacgctg gcgcgtctta tccgacctgc ggggacgcac gtgtaggccg gataaggcgt
    16921 ttacgccgca tccggcaatg gtgtccaaat gcagcacgtt ttatccgttc tggacttccc
    16981 ctgccaacca acgcgccgca acaataaccc cctgccccag agacaacccg ccatcgcccg
    17041 caggtaaact ctgtgggaag agcaacgtaa aatcagcaag ataatgcgcc agacgtgcac
    17101 gcagcaaacg gttatgaata actccgccgc taaataccag cgtagtgata ccccgcatcg
    17161 tggcctgctc acgcatcaac gcggcaaaac cctgcgccag cgcatcatga aacgcccacg
    17221 cgcgttgatt aaccggagcc tgccagctca gccactgctg ccagaaagtg gcgagatcca
    17281 gttgattgtc caccagcggc atcgtcaccg gatgcgtcac tctgtggcac ggagctgcga
    17341 gtgcctccag agcacaagcc gcttcacctt cataacttaa cgtggctggc gcacagccca
    17401 gcgccgccgc tacggcatcg aacaaacgtc cacacgatga tgccaacggc gcgttaattc
    17461 cacgctcaat agcccgcgcc agcacgctcc agttttgctg ttgcacactt gccgtttcag
    17521 agtaattctg ccactccggc acaaagcgca ggcactgcgc cagcaggttt cgccacggct
    17581 gcttcgcagc caaatcgccc cccggaagcg ccactgcggg caagccgccc aggtgctggc
    17641 attcgcgata gttcacccgc aagcactcgc cgccccacaa agcgcagttc tcccccatac
    17701 caataccgtc gagcgtcaac gcaatgacat ctccgccatc cagaggccag aggtgttctg
    17761 ccagacacgc cgctgcgtgg gcatgatgat gcagcaccgt ttgcgtcggc agattcatct
    17821 cacgcgccca ctggctggag acatagcccg gatgcgcgtc atgcacaacg tattgcggag
    17881 tgaaatcgta gatgttttgc atcaggcgta acgcttcgcg ccactgcatc tggatgccat
    17941 cgtcacttaa atcgcccaga tgctgactca acaccgcttg ttcgccgcgc accaggcaga
    18001 aggtattttt cagatccgcg ccgagacaca gcacaggcgg aacattttta aagcccggag
    18061 gcaaagccag cgcatccggc acatatcccc gcgaacggcg cagcatttcg ccgctttcgc
    18121 gcaccaccga atcatccatc cgctgcacga tgtcgcggtt atgtatcaag aatccgtcgg
    18181 caatgccctg caaatccgcc agcgcctgtt cgttgctgat agccggtggt ttaccgctca
    18241 ggttgccgga ggtcatcacc agcgggcatt gcagttcctg taacagcaaa tgctggagcg
    18301 ggttcgcagg caacattacc ccgacttcgt taaggccagg ggcgatatca tcacaaagct
    18361 caggaacgta ttttttatcc accagcacaa tcggcgcggc gggcgtggta agcaactgac
    18421 gggcagcgtc tggtaaacct tctgccgttg gcaacatgac cgccagcggt ttcgccgggc
    18481 gatgtttgcg cgcccgaagt gtcgccaccg cgttactgtt acgtgcatcg caggcaagat
    18541 gaaatccgcc aatccctttg atggcgacaa tgttgcccat ttttaactgt gcgatagctg
    18601 cctgtaatgc cgcctcttgt tctgcatgtt caccatgact tacccattca agatgggggc
    18661 cacactccgg gcaggccacc ggctgggcgt ggaagcgacg atcgagcggg tcacagtact
    18721 ctttgtcaca agccggacac agcggaaacg ccgccatcac ggtaaacggg cggtcgtaag
    18781 gcatggcgcg aataatggtg aaacgcgggc cacagtgggt acagttgata aacggataac
    18841 gccagcgtcg ttcgcctggg gtattcattt cggcaaggca agcagggcaa gtcgcggcat
    18901 cgggaacaat ttgcgtattc atgacgccac ccgcgctctg gcggatggtg aactcagtgg
    18961 gcagttgtga ccagataaac ggctcacgct cgacgctatc aatacgcgcc agtggcgggc
    19021 agtgctgatg caattgaaca agaaacgttt ccggatcttc cagcagccgg acttctacgc
    19081 catcgccgtc attacagaca tcgccgtgaa gatttaattg ctgtgccagt tgccagacaa
    19141 acggacgaaa accgacgccc tgcactttgc cacgaatacg cagttggaca ccgcaagatg
    19201 tgttttttgc cattgagtta ttcccgccat catgaattgc gtaacccgcc cagccggaca
    19261 cgacagcgtc gcatccggca gtcacaggtc ggcgatactg cccgcttcgt attctacgaa
    19321 tatttccggg aattcctttg ataccagaac agttctgtaa gatttttaga acatcagcgc
    19381 cgtgcggcgg cgtttttctg cgctcagttg ttcaagttta ttacgatcga cacaaatcag
    19441 cgcatgggtc gggcaagccg ccatacacgc cgggccgtct tcacgatggt tgcacaggtc
    19501 gcatttattg gcttcggctt tgtcagcccg tacattcagc cctgcgccgc tgttgcgaat
    19561 caccggacgc accaccactt ccatcgcacc atacgggcaa gccacaacgc aggttttgca
    19621 accaatacaa cgttcctgca tgacatgaac aaacccttta tcacggctga tagcaccatt
    19681 cgggcagacg ttagcgcacg gtgcatcttc acactgacgg caaactgtcg ccgtggaaat
    19741 gttcacacct ttaatgacat ggatacgcgg taaaaatgtt tccggggtca gcgatgcaca
    19801 gtcctgattt tcctgatgag aaaccacgca cgccacttca caggtacggc aaccaataca
    19861 tttactcgcg tcagcaatga tgaaacggtt catcaaattc tccagcaata acagttaatg
    19921 cgccgataca ttcacaaatc atgccagttt ttaatttact gttatttaaa gaaattaaat
    19981 tctgtaatgc aggaaaaacg atgtcatcga cactagtgac gatgacatgt gatgacagta
    20041 tttatcgttg ccagtccaca tctgggtact tttttccgac aagtcgttct ggtggtataa
    20101 aaaagtaatt gccagtaacg gggaggattt aagggaagca accgtgccgg atacggcgtg
    20161 aacaatgacc actttgtcag ctatccatat gattgtattt tgaggttcat cgcagattag
    20221 cgatgaacct ttattaaata atacaattcc gactgaaata acaggtaagg atacaaaatg
    20281 ggttcatggg gaatggaggc tctcgaaagc gacgaagggc tggatttgat taattgggta
    20341 gaggaacaac tgcaagatga ttcgaccttt gatgccgagt caattgttca acgcttaagc
    20401 cagcatgaag atttgttcgg atttcaggga gatgaagaat ttctctacga taataatgtt
    20461 atcggtctcg tcgaacttat cattcaaaaa gcagccgggg aaaaaatcac atcgagtaaa
    20521 caaattgatc aactcgacag ttatcggctc acgtcgattt tcagcaaaaa actccagggc
    20581 cgtttacaaa caatcgacga cacacatgag tggataatgc tttttgaggg gcgagcacgc
    20641 gaaaaggcaa aagcatatct gattgagatt actgataaat taagagtagt aaagacaaca
    20701 gcctgaatga caaacaaggg cgcgcaaaat taagaatctg cgccctttca aagcccgaca
    20761 ggcgtagcgc atcaggcgat ttagcgtctg ccatcagtca aagaaaccat tccggcttaa
    20821 cgccaaatcg ggctgaaagt gccttaatgt gggatatcgt caaggatcgt tgtccagaca
    20881 agatctggct gaccagggat ttagagccga tttcgttctt caaatccgca taggtcagat
    20941 tatgttgatc aatcagcgtg cgaagtaacg ccgcaccaac aggcattgcc gcaacggctt
    21001 tgtcgaactc tgcaaacttt tcgttgttgt tttcatattc tgcaatgcgg ctcgtcagaa
    21061 agtcgataag tggattggtg tcatcttctt caatcaaata ttcgaccagt tccagtgctt
    21121 cacgataatc atcttctgag tcactgcctc ccagaaaggg gactgcattg accagttctc
    21181 ttgttgcttt aacagcgcgt acagcattag cagtcattct ttgttctctc tgtagtaacg
    21241 tgtcagtttg tcatactcgg cgtgattagt tatgtgcttc acaaagaacc gcttattaac
    21301 aaagtttatg taggcaatca cccttaggtt gttcccacct acatccagaa cgtaccattt
    21361 gttgcgatat ttaaaattgt cgaggttggg aaacgccgtt cgcatttctt ctggcgtaga
    21421 aaaatccgtt tccttaataa cccgatacaa tgcctgaaga gccagagcgt cgttaggata
    21481 tttacgcgca gactcttcaa atggcgcttt agaaatgatg tgcattttta agttttaact
    21541 tccttatagt aagtaaacag ccacatctgc gagttctatc cttatatgtt cggaacacta
    21601 tagcttatca aatccaagtt cacaatatgt aaacacttgc gcacgatata attactacaa
    21661 aaatcctcat agccactctg caaaaagtca gttctgattc accccatcaa acacatcacc
    21721 ctttattaaa caacgctgtg tcagcgccag atctttcgtt tgcgggttac gtccggcgac
    21781 aggtttaatg acgagtttac ggttggaggt ataggtaaaa cgcgtgagtg cggggtagct
    21841 taacttgcca aaccagccgt gaacgcgctg ccatttggcc tcttttacct gatacaaact
    21901 ggttatccag taaccggaat caaactgcat atccagcaat tgcgtgtgac cattgccttg
    21961 taaatcaagc aaatcatcaa cgccagtttc gtttgcggtg taaaaaccat cgacctggaa
    22021 aacacacggg cgaccttgct gattaaaggt catcaggatc aaaaacgagg aaggcgccag
    22081 cccgtttccc gaaacacctc gccagataac caaatcctga atgccattac gatcgaggtc
    22141 agcacggtaa aattgcgcat tacccgccat attttgcagc acacccacag gcacaaacca
    22201 ggatttaccg gcacggtcct tagcggttaa tcgccagtaa tcatcttcat ttggcatctt
    22261 ctcaagcgtt acgcctgcaa cgcccacttt tctaaaatgc cagttttgat cggcatcata
    22321 aggcatggtc aggagaggtt tcagcgcccg cttttgcgcg tattcgctgt cactcattgg
    22381 cagatattcg gtaaagctgg tggcctgtgc aggcagcact aagctcaata aaaaagcgca
    22441 catccctgtc ctgatgttca attccctgaa ttccttactg aaaaactact cttcttccgc
    22501 cgctaactgc gcgaagccgc catttccttc ccagccttct agccgctgat acaccgtttc
    22561 caccgcatct ttaatctgct gagtcatcgg gtagtaaaag ccgacgatat ccggctggat
    22621 gccaaggaaa atcacttcgc caatatcttc tttcaactgg tcgataagat agttcagcgg
    22681 catgttatgg gtagtcatca taaacatctc ggcgatgtca gccgggtcga tgatgcggat
    22741 ctcgccaggg tttagcccca tatccgtggc gtcgacaata agcagccgtg tcgggcgcag
    22801 ttcgcgaata gcgacaatgt cgttttccgg tgcgctaccg ccgtcaatca ctacccagtt
    22861 acctttcggc gcggcagcgc acttttccgc cagtagcggg cctgcgccat catcgcccat
    22921 catgctattg ccaacacaga gtaaaacgtc agtcacgcag tctcctcacc atcaaataga
    22981 tggcgttttc ctgatgaata tcatgcagca tacttaacag cattgtactc cattccttct
    23041 gctgcgaagt ttgcgctgcc cgggctttat cgaatgcgtt ggccagcatc ggcacatggt
    23101 taatgtcgat gacgatctca ccatatttcg gcacgccttc cattttacgg cgggcttcgc
    23161 tgcccgcctc cagagtggca atccatgcca gatattcatc ccacgggcag gtaagcgccg
    23221 cttccaggca atcgataacc ccaaggtggt gaccaatcgc caggctgtaa tagaccacct
    23281 gctgcgcctc ggcgggcgtg gcatcgttct catcaatgaa tttacggctc agttgactga
    23341 acaccacctt ttcactcatc ggatacgcgc ctcttcaaca acatgattca gatggctgac
    23401 aatctcgttc agacgcggat cgttttccgc ttccagccag cgtgcaacct gttcttcgcc
    23461 ctgccctaac tgtgtcagga agtcatcggc aatctgacga ccgtaacgat aacccgccag
    23521 tcggcgtgct tcgcgatcca ctttcacgcg cagcggctgc accatatcgc catgcagaat
    23581 ctccgccggt tgttcatcct gctcacccgg cccacgggcg tgaattttct gctccagcag
    23641 gccgagcgcc atcgcaaagc cgtacagcgt ggcggcaggc gttggcgggc accccggaat
    23701 gtagacatcc accgggacga ttttatccgt accgccccac acgcagtaga gatcgtggaa
    23761 gatcccgccg ctgttaccgc aggcaccgta ggagatacag attttcgggt ccggcgcgga
    23821 ctgccacgca cgcagcgcag gggatcgcat tgcacgggtg accgcgccgg taaacagtaa
    23881 aatatccgca tgacgcggtg acggaacaac tttaatgccg aagcgttctg catcgaacag
    23941 cggcgaaagc gtggcgaaaa tttcgatttc gcaaccgtta cagccgccgc agtccacgcg
    24001 ataaacgtag gcagaacgtt tgattttttt cagtaacgac gccttcatgc tgtcgatgga
    24061 ttcatccacc gtcatcggga ccggaatgcc gttggcgtca cgggggccta ataaattgct
    24121 catcagatgg cctctttcat atggcgagtc agttcaatac ggtcggacgg caccaggcat
    24181 ttctggcgct tacattccgg gcaagtctca aagctttcgc ggtggttttc cgcgcggctg
    24241 tcgccgttgt gcttaagcag cgcaatggcg tagtcgatct ctttctggac ggcgaaagga
    24301 cgattgcaga cgcggcagtt gcacagcgcg aagcgggact gttgcaggaa gtcttctttc
    24361 ttccacaccg ccagttcgta ttcttgcgac agtttgatcg ccgctgtcgg gcagacttct
    24421 tcgcagcgcc cacagaagat gcagcgccca aggtcaaact gccacgccag ctcaccagtg
    24481 gcgaggtcgg tttcaaccgt taaggcgttt gacgggcagg cattgacgca agccgcacag
    24541 ccaatgcact gctgcgggtt ctgctctggc ttaccacgga agtttttatc aaccgcaatc
    24601 ggctccagcg gataagacga agtcgccgtg ccggttttga tgactttttt gataaaggta
    24661 aacatggcga ttccttattt cagcggcgag tttttacgct caatgctgta acgctcgagt
    24721 tctttgtacg gcaccacttt gctcttcttc ttacgtacat cgaccacggt catgcggtcg
    24781 gtacaggagt agcaagggtc caggctaccg ataatcaacg gcgcatcgga aacggtgtta
    24841 ccgcgcagca tgtagcgcag ggtcggccag ttcgcgtagg tcgccgcacg gcagcgccag
    24901 cggtacagct tctggttgtc gccggtcatg ctccagtgga tatcgtcgcc gcgcggcgct
    24961 tcggcaaagc ccagcgcgaa acggtgcgga atgtaggtaa agccttccac catcagcggg
    25021 ccgcccggca agttatccag accgtaatcg atcatattca gcgcggtata gacttcgttg
    25081 atacgcactt tcagacggga aataacgtcg cagccctgct cgctgtggac ttccattggc
    25141 agcaggccat aaccaacaaa cgggtgatcg gcacgggtat cacgggcgtg accgctggca
    25201 cgaaccatcg ggccaacgtt actgaagtcg cgagcgattt ccgggtccag acgaccaatg
    25261 ccgacggtgc gttgttccat gttcggtgtg ctcagcagca catccaccag ctcctgcact
    25321 tcacgacgca tctgttgtgc cagctggcgg gtctggatca tgtcgtcttt cagcagatcg
    25381 cgacgaatcc cgccgatcag gttcaggccg taggttttac gtgcaccggt aaggatctct
    25441 gccattttca tggaggtttc acgcacgcgg aagaactgca taaagccgga gtcgaagccg
    25501 gtaaagtgac aggccaggcc gagattgagc agatgcgagt gcaggcgttc tacctccagc
    25561 agaatggcgc ggatcatctg cgcgcgttct ggcacctgaa tacccatcgc gttttccacc
    25621 gacgtggtat aggcggtgct gtgagcaaag ccgcagatcc cgcacacacg gtcagagagg
    25681 aaggtcactt cgttgtaacc catacgggtt tccgccagtt tttccatacc gcgatggacg
    25741 tagaacaggc ggtagtcggc gtcgataatg ttttcaccat cgacgaacag acggaagtga
    25801 cccggttcgt cagaagtgac gtgcagtgga ccaatcggca cgacgttgtt tttcttgtcg
    25861 cccagttcgt tgatgaactc gtaggtttca gcatcggtag tcggtgccgg acgctgacga
    25921 taatccatgc tgtctttacg cagaggataa agttcatccg gccagtcatc cggcagcacc
    25981 agacggcgtt catccggcag accaaccgga atcaaaccgt acatatcgcg cacttcacgc
    26041 tcgccccaca ctgccgccgg aacgcgcggc gtcacggaag gatactccgg tttgttggcg
    26101 tcaacttcga cgcgaaccgt tacccaacac ttggtgccct tctccatcga cagcacgtaa
    26161 taaacggcgt aatgaccatt cagtttgcgt tcgtcgttac caaacaacac cgacagccag
    26221 ccaccctgct tgtagtagag aaactccacc acttccggca ggtagttcac ctttacggtc
    26281 acggtcagct gatctttggt ctgccaggcg tggtccagca cgacgccagg aaatgcctca
    26341 ttcagcgcgg cgagataatg ttgacctaat ttttcttcag acatgctcaa actctcttta
    26401 atcacgccgc cagcaaggag acgaacgcta aaaatgcaaa gccaaacccg gcccaggtaa
    26461 tgcgcggagt aatatcaaga cgcagacggg ccatgctgtt ttcgaacagc gcgataacca
    26521 ggacgccaac caccagtttg acgatggcaa tcaccagcgc cagcagcagc ccaccgacgg
    26581 tgaaggtttc catttgtccc cacggaataa acaccccgac gaacatctgc aacaccacca
    26641 gctgtttcag gctgatgccc catttcatga cgccaaagcc gctgccgcta tactcagaga
    26701 gcggaccttc ctgcaactcc tgctcggctt ccgccaggtc gaacggcagt ttgcccattt
    26761 cgataaaagt ggcgaacgca caggcacaaa gcgccagcac cagcgggatg ctctggctca
    26821 gtggccagtg ataaacggtg tcggtgatgt tgctgatgtt ggtggaaccg gcaacctgtg
    26881 cggcgaccca cagaccaagc agcagcatcg gttcaaccaa cacgccaagc atcgcttcac
    26941 ggctcgcgcc gatagcggta aacgggctac cagtatccag accagaaatg gcaaagaaga
    27001 aacgcgcgat ggcgaagaga tacagtaagg tgatcaaatc gcccagttgc ggcagcggag
    27061 agccgacggt caccaccggc agcgcagtgg cgatagtcag catgacgccc accatcacat
    27121 acggcgtcag gcggaacacc cagccggagg catccgggcc aacgctctgg cggcccagca
    27181 gtttgataat gtcgcgatac tcctgcaaca cacccggccc acgacggtta tgcagacggg
    27241 cgcgcgctac gcgggtaata ccggagagca gcggcgcaac ggcaaatagc accagcgcct
    27301 gaattaacgg atataaaaca ctcattctca ggctcctcgt gaaacaataa tcaccaccag
    27361 caccgccagt tcaaccagcg ccatccggcg gaacagcaac gcactcccct cgcactgcca
    27421 gcccggcacc agagacaccg gattcagcca tttgcgtagt ttcagcaccg gcgcaaacgc
    27481 ctgtttcacc ggcatggcaa aaccgtgagc ggtaatcacc attgattttt catgatcgta
    27541 gccgcacacc caggccgcac cgcgggaacg cgacggcaaa cgatcgcctt tgcaaatcgc
    27601 cataatgatg aatggcagca gcgggcaggc aatcagcagc aacgtgatca ttggttgaga
    27661 aacggtggtg ttagcaggct ccagcggcag aggtacagca gcagagagca tcggcagtag
    27721 ccacggcgca gcaacaccgc caattacgca gcaaatcgcc agtgccacta cgcttacgct
    27781 catcaggagc ggcgcacagg tggcgttttc ggcctctttg gtacgcggcg cgccgaggaa
    27841 agtaacgcca taaactttcg ccatacacat caccgccagc gcaccggtaa ttgccagccc
    27901 cacggcgagc agcggcccga gaagacgggc aacaaacgcg ccactattgc tcagtttgaa
    27961 gaaggattga tagataaccc attccccggc aaaaccgttc agcggcggca gcgcagccat
    28021 tgccatcagc ccgactaaca tggcgatgga gataaccggc attttcttgc caataccacc
    28081 gagtttttcg atatcgcgat gaccggtacg gaaccagacg ctccccgcac caaggaacag
    28141 tacgctcttg aacaggctat ggttaatcag atggtacaga ccaccaacca gaccaagggc
    28201 aatcagcgcc ggttgttcga gcgcgatacc cgttacgcca gcgcccagcc ccagcaggat
    28261 aatgccgata ttctccaggg tgtggtaagc cagcagacgt tggatattgt gttccatcaa
    28321 cgcatacaga ccgccgacga atgcggtgat cataccgagc accagcagcg cgatccccca
    28381 ccacagcggt gcgttaccgc ccagcagtga cagggttaaa atgcccagca ggccaatttt
    28441 catgactacc gtagaaaaca acgcggcagc tggcgcagag gcgttagcat gtgcctgtgg
    28501 cacccagccg tgcagcggaa taatcccggc cagcaggcca aagccaatca caccgagcag
    28561 ccagatatcg gaaccgagcg gcagctgttg catacgcata tccagcaggc gcagatccag
    28621 cgtgccgtaa cgctgccaca gcagccagca ggcaatcgcc agcagcagag tgccaagacg
    28681 ccccagcgca aaccacagtt tgccctcttt gctgttgctg gtgaggaaca ccgcacacag
    28741 ggccatgatt tcggccatta ccacgaacat gccgaggttg ctggcgatga cggcacagac
    28801 ggcggcagcc atcaacatat tgatctgcaa gccgttgcat ttcacctgcg cgtggcgatg
    28861 ccagtcaatg ttgtagaggc tgacaaacag accgcacaga ccgagcgtaa tcagccaaat
    28921 cgcgttaagc ggagagattt gcacatcgta gcttaccagc gacaacgcac cgctcacgcc
    28981 aaccgcgcca gtcagcacag taaagcccgc ggctgccgta tacaggctac ccaccgcgcc
    29041 gccaattccg gctatccagc cgcttagcgc cttttgaaaa gaaaagagaa atgccagaac
    29101 agcggcggcg acaaaccacg ccacgccgct attgatcagg gaaattgcgc tcatttagcc
    29161 tctccacttt gagcctgctg aaacaaggtg agatcgccaa agtccgtgtt aaaagtcagc
    29221 tcacgcttac gtttgctgac gcgggcgata tcggtgttat ccaccagatg cagggcttta
    29281 gtcgggcaca tccgcacgca ggccggacct tgttcatcaa agctacaaag gtcacatttg
    29341 acggcgatcg cgcgaatacc tggcacccag tcaagcaatg tgctgacacg cgccggagca
    29401 ggcggtgccg gtggcgcttt cggggtattg gcgtttgccg gaatatccag cggacggctg
    29461 ccggaaaatt caattgcgcc aaacgggcag gcgataccac acagtttgca gcttacgcac
    29521 agactttcat tcaactgcac ggccccatca acgcgggtga tggcgttaac cgggcagacc
    29581 gttgcgcagg gtgcatcttc acagtggtga cagagctgcg gcgcagattc tttttcattg
    29641 agcatcactc tcaggcgcgg cattgattgc aggccgtgct ggcgatgcgt ctctgaacag
    29701 gcagcctcac aagtgtggca gccgatacag agcgtggagt cagcaattac aaaacgattc
    29761 accaggcatt cctcaggtga ttgtcatttt tgacgaaaac atgccgatga aatgtcattt
    29821 tcgacactca tcgacacgcc catccccaaa caggcgtaac gcctgcaaaa cgggcaaagc
    29881 ctcagctcat gctgccgggc tttgtccctt taccagttgg cttaaattca ccggcacatt
    29941 gttttcaatg gcggtatata agctgtcgta aacgccagcg attttttcga gatagtgttc
    30001 cagaacctgt tcgcgatcgc ggatgttgac cttgccatca atcatgccgt caatgctcag
    30061 ttgcgcctgc gcaatccgct gtttgtcttc gccatctttt gtttcgacgt aatactcgat
    30121 ggtgtggctg ttaaagccat ccgccagggt gacgtaaatg cgaaaatgtt caaaaaggac
    30181 gaaacagagt tctttgtccg gcgcgcagct catggcgtga tgcaggcgcg ctttcgataa
    30241 cgtgatcccc tgaaccagcg aattgcagta gatgtgccac tggtcctgta ggcgacgatg
    30301 ccgctgtgcg atgtaatcgg ctttctcgct tatttcccaa atagtcattg tcaggttacc
    30361 cgtttaacag agatgccagc tttaagcatt ttctgtgcca acttttaatt cattgttatt
    30421 aaatcgattt ctaagctaaa gatgaatttc gtcgccgtgt cgacgtgtca tttcgacatc
    30481 atcgacatta ttcaccgcag ggataatcaa cactggcaca attattgctt atagctggca
    30541 atagttaatg ggaggcgata tgcacgaaat aaccctctgc caacgggcac tggaattgat
    30601 cgaacagcag gccgcaaaac acggcgcaaa acgcgtaact ggggtctggc tcaaaattgg
    30661 cgcattttct tgtgtcgaaa ccagctctct tgccttttgt tttgatctgg tttgccgcgg
    30721 cagcgtggcg gaaggttgta aactgcacct cgaagaacaa gaggccgaat gctggtgtga
    30781 aacatgccaa cagtatgtga cgctactgac ccagcgcgtc cgccgctgtc cacagtgtca
    30841 tggtgacatg ctacagattg tggcagacga cggtttacag attcggcgga tagaaataga
    30901 ccaggagtga gcgatgtgta caacatgcgg ttgcggtgaa ggcaacctct atatcgaggg
    30961 tgatgaacat aaccctcatt ccgcgtttcg tagcgcgcca tttgccccgg cggcacgccc
    31021 gaagatgaaa atcaccggca ttaaagcgcc tgaatttacc cccagccaga ctgaagaagg
    31081 cgacctgcat tacggtcatg gcgaagcggg cactcacgcg ccgggcatga gccagcgtcg
    31141 gatgctggaa gtcgaaattg acgtgctgga caaaaataac cgtctggctg aacgcaaccg
    31201 cgcacgcttt gctgcccgca agcaactggt gctcaacctg gtttccagcc ctggttccgg
    31261 taaaaccacc ctgctgacgg aaactttaat gcgcctgaaa gacagcgttc cgtgcgcagt
    31321 tattgaaggc gaccagcaaa ccgtgaacga tgccgcgcgc attcgtgcta ccggcacgcc
    31381 agcgattcag gtgaacaccg gtaaaggctg ccatcttgac gcacagatga ttgccgacgc
    31441 cgcgccgcgc ctgccactgg acgataacgg tattctgttt atcgaaaacg tcggcaacct
    31501 cgtgtgcccg gccagcttcg atctcggtga aaaacacaaa gtggcggtgc tttccgttac
    31561 cgaaggtgaa gacaaaccgc tgaaatatcc gcatatgttt gccgccgcct cgctgatgct
    31621 gctcaacaaa gttgacctgc tgccgtatct caactttgac gttgagaagt gcatcgcctg
    31681 cgcccgtgaa gtcaatccag aaattgaaat catccttatt tccgccacca gcggcgaagg
    31741 gatggaccag tggctgaact ggctggagac acagcgatgt gcataggcgt tcccggccag
    31801 atccgcacta ttgacggcaa ccaggcgaaa gtcgacgtct gcggcattca gcgcgatgtc
    31861 gatttaacgt tagtcggcag ctgcgatgaa aacggtcagc cacgcgtggg ccagtgggta
    31921 ctggttcacg ttggctttgc catgagcgta attaatgaag ccgaagcacg cgacactctc
    31981 gacgccttac aaaacatgtt tgacgttgag ccagatgtcg gcgcgctgtt gtatggcgag
    32041 gaaaaataat gcgttttgtt gatgaatatc gcgcgccgga acaggtaatg cagttaattg
    32101 agcatctgcg cgaacgtgct tcacatctct cttacaccgc cgaacgccct ctgcggatta
    32161 tggaagtgtg tggcggtcat acccacgcca tttttaaatt cggcctcgac cagttgctac
    32221 cggaaaacgt tgagtttatc cacggtccgg gttgcccggt gtgcgtactg ccaatgggta
    32281 gaatcgacac ctgcgtggag attgccagcc atccggaagt catcttctgt acctttggcg
    32341 atgccatgcg cgtaccaggg aaacaggggt cgctgttgca agcaaaagca cgcggtgccg
    32401 atgtgcgcat cgtttactcg ccaatggatg cgttgaaact ggcgcaggag aatccaaccc
    32461 gtaaagtggt attcttcggc ttaggttttg aaaccaccat gccgaccacc gccatcactc
    32521 tgcaacaggc gaaagcccgc gatgtgcaga atttttactt cttctgccag cacattacgc
    32581 ttatcccgac gctgcgcagt ttgctggaac agccagataa cggtatcgac gcgttcctcg
    32641 cgccgggcca cgtcagtatg gttatcggca ctgatgccta taattttatc gccagcgatt
    32701 ttcatcgtcc gctggtggtc gctggtttcg aaccccttga tctacttcaa ggcgtggtga
    32761 tgctggtgga gcagaaaata gcggcccaca gcaaggtaga gaatcagtat cgtcgggtgg
    32821 tgccggatgc cggtaacctg ctggcgcaac aggcgattgc cgatgtgttc tgtgtcaacg
    32881 gcgacagcga atggcgcggc ttaggcgtga ttgaatcttc tggcgtgcac ctgacgccgg
    32941 attatcaacg attcgatgcc gaagcgcatt tccgcccggc accgcagcag gtctgcgatg
    33001 acccgcgtgc gcgttgtggc gaagtcttga cgggcaaatg taagccgcat caatgcccgc
    33061 tgtttggtaa cacctgtaat cctcaaaccg cgtttggtgc gctgatggtt tcctccgaag
    33121 gagcgtgcgc cgcgtggtat cagtatcgtc agcaggagag tgaagcgtga ataatatcca
    33181 actcgcccac ggtagcggcg gccaggcgat gcagcaatta atcaacagcc tgtttatgga
    33241 agcctttgcc aacccgtggc tggcagagca ggaagatcag gcccgtcttg atttggcaca
    33301 gctggtagcg gaaggcgacc gtctggcgtt ctcaaccgac agttacgtca ttgacccgct
    33361 gttctttcct ggcggtaata tcggcaagct ggcgatttgc ggcaccgcca atgacgttgc
    33421 ggtcagtggc gcaattccgc gctatctctc ctgtggcttt atcctcgaag aaggattgcc
    33481 gatggagaca ctgaaagccg tagtgaccag catggcagaa accgcccgcg cggcaggcat
    33541 tgccatcgtt actggcgata ccaaagtggt acagcgcggc gcggcagata aactgtttat
    33601 caacaccgct ggcatgggcg caattccggc gaatattcac aggggcgcac agacgctaac
    33661 cgcaggcgat gtattgctgg ttagcggtac actcggcgac cacggggcga ctatccttaa
    33721 cctgcgtgag caactagggc tggatggcga actggtcagc gactgcgcgg tgctgacgcc
    33781 gcttattcag acgctgcgtg acattcccgg cgtgaaagcg ctgcgtgatg ccacccgtgg
    33841 tggtgtaaac gcggtggttc atgagttcgc ggcagcctgc ggttgtggta ttgaactttc
    33901 agaagcggca ctgccggtta aacctgctgt gcgcggcgtt tgcgaactgc tgggactgga
    33961 cgcccttaac tttgccaacg aaggcaaact ggtgatcgcc gttgaacgca acgcagcaga
    34021 gcaagtgctg gcggcgttac attcccatcc attgggaaaa gatgcggcgc tgattggcga
    34081 agtagtagaa cgtaaaggtg ttcgtcttgc cggtctgtat ggcgtgaaac gaaccctcga
    34141 tctaccgcac gccgaaccgc ttccgcgtat atgctaataa aattctaaat ctcctatagt
    34201 tagtcaatga ccttttgcac cgctttgcgg tgctttcctg gaagaacaaa atgtcatata
    34261 caccgatgag tgatctcgga cagcaagggt tgttcgacat cactcggaca ctattgcagc
    34321 agcccgatct ggcctcgctg tgtgaggctc tttcgcaact ggtaaagcgt tctgcgctcg
    34381 ccgacaacgc ggcgattgtg ttgtggcaag cgcagactca acgtgcgtct tattacgcat
    34441 cgcgtgaaaa agacaccccc attaaatatg aagacgaaac tgttctggca cacggtccgg
    34501 tacgcagcat tttgtcgcgc cctgatacgc tgcattgcag ttacgaagaa ttttgtgaaa
    34561 cctggccgca gctggccaca ggtgggctat accccaaatt tggtcactat tgcctgatgc
    34621 cactggcggc ggaagggcat atttttggtg gctgtgaatt tattcgttat gacgatcgcc
    34681 cctggagcga aaaagagttc aatcgtctgc aaacatttac gcagatcgtt tctgtcgtca
    34741 ccgaacaaat ccagagccgc gtcgttaaca atgtcgacta tgagttgtta tgccgggaac
    34801 gcgataactt ccgcatcctg gtcgccatca ctaacgcggt gctttcccgc ctggatatgg
    34861 acgaactggt cagcgaagtc gccaaagaaa tccattacta tttcgacatt gacgatatca
    34921 gcatcgtctt acgcagccac cgtaagaaca aactcaacat ctactccact cactatcttg
    34981 ataaacagca tcccgctcac gaacagagcg aagtcgatga agccggaacc ctcaccgaac
    35041 gcgtcttcaa aagtaaagag atgctgttga ttaatctcca cgagcgggac gatttagccc
    35101 cctatgaacg catgttgttc aacacctggg gcaatcagat tcaaaccttg tgcctgttac
    35161 cgctgatgtc tggcgacacc atgctgggcg tactgaaact ggcgcaatgc gaagagaaag
    35221 tctttaccac taccaatctg aatttactgc gccagattgc cgaacgtgtg gcaatcgctg
    35281 tcgataacgc cctcgcctat caggaaattc atcgtctgaa agaacggctg gttgatgaaa
    35341 acctcgccct gaccgagcag ctcaacaatg ttgatagtga atttggcgag attattggcc
    35401 gcagcgaagc catgtacagc gtacttaaac aagttgaaat ggtggcgcaa agtgacagta
    35461 ccgttctgat cctcggtgaa actggtacag gtaaagagct gattgcccgc gcgatccata
    35521 atctcagtgg gcgtaataat cgccgcatgg tcaaaatgaa ctgcgcggcg atgcctgccg
    35581 gattgctgga aagcgatcta tttggtcatg agcgtggggc ttttaccggt gccagcgctc
    35641 agcgcattgg tcgttttgaa ctggcggata aaagctcact attcctcgat gaagtgggcg
    35701 atatgccgct ggagttacag ccgaagttgc tgcgtgtatt acaggaacag gagtttgaac
    35761 gtctcggcag caacaaaatc attcagacgg acgtacgttt aattgccgcg actaaccgcg
    35821 atctgaaaaa aatggtcgcc gaccgagagt tccgtagcga tctctattac cgtctgaacg
    35881 tattcccgat ccacctgccg ccactacgcg agcgtccgga agatattccg ctgctggcga
    35941 aagcctttac cttcaaaatt gcccgtcgtc tggggcgcaa tatcgacagc attcctgccg
    36001 agacgctgcg catcctgagc aacatggaat ggccgggcaa cgtgcgtgag ctggaaaacg
    36061 tcattgagcg cgcggtattg ctaacacgcg gcaacgtgct gcagctgtca ttgccagata
    36121 ttgctttacc agaacctgaa acgccgcctg ccgcaacggt tgtcgcccag gagggcgaag
    36181 atgaatatca gttgattgtt cgcgtgctga aagaaacaaa cggcgtggtt gccgggccta
    36241 aaggcgctgc gcaacgtctg gggctgaaac gcacgactct gctgtcacgg atgaagcgac
    36301 tgggaattga taaatcggca ttgatttaac agatgtggtt atactcgatc aacattcaat
    36361 gaaaggaggc agtatggcag tttcagcaag aaatcaatta accggaacag ttagcgcagt
    36421 agcaatgggc gctgtgaatg atgaagtaga actgacgctg gcaggtggtg caaagctggt
    36481 ggcaattgtt acacacagca gccagcaagc gctgggtctg gcgaaaggga aagaagcgat
    36541 tgccctgatt aaagccccgt gggtcacgct ggcaacggaa gactgtggac tgaaattctc
    36601 tgctcgcaac cagtttgctg gcagcgtgtc gacaatcacc gaaggcgcag tgaacgccac
    36661 tgtccatatc aaaacggatg cgggttttga aattgtggca gtggtaacaa atgaaagtca
    36721 ggatgaaatg aaactcacca ctggctcgcg cgttattgca ttgatcaaag catcagccat
    36781 cctgattgcc accaaagcgt aaacgcctta tttgccggag agtatggctc tccggcagat
    36841 ttatttcttt ggacgatatt tttctggcaa ctccggcacc ggacgcttgt catcgatgag
    36901 atgacgcacg gttaagatcg gatgacgcca cagcattctc ggcccggccc aacgcataat
    36961 ctgtttcatc tcttcacgct ttgcaggctg gtaacagtgc accggacact gcttacaggc
    37021 tggtttctct tcaccgaaca cacatttatc cagccgcttt tgcgcgtaag caaacaacgc
    37081 ctcgtaatgc tccggctctg ctgacgcctg cgggcatttc gcttgataaa gatcgatcat
    37141 ttttttaatc gtcagttttt cacgagagat acgcttgccg gacatgctgc ctccatactc
    37201 ttaaggtgca tttatattac aacttaattt taaaggggac tatggctctg aaggaggagg
    37261 tttcttcaac cgacgaattt ggcgatcttg tttcatcgtc atcacgcaaa agctgcaaag
    37321 cagcattttt cgcggaaccg acatcaagaa ctcacctttc gcgtcttccc ctgaaatgat
    37381 taactccggt atcatgtgcg ccttatgtga ttacaacgaa aataaaaacc atcacactac
    37441 atttaacatc agggagccgg acttaacccc atgagtacaa tagaaaattt cgacgcccat
    37501 acgcccatga tgcagcagta tctcaagctg aaagcccagc atcccgagat cctgctgttt
    37561 taccggatgg gcgactttta tgaactgttt tatgacgacg caaaacgggc gtcgcaactg
    37621 ctggatattt cactgaccaa acgcggtgct tcagcgggag agccaatccc gatggcgggg
    37681 attccctacc atgcggtgga aaactacctc gccaaactgg tgaatcagag cgaatccgtc
    37741 gctatttgtg aacagattgg cgatccggcg accagcaaag ggccggttga gcgcaaagtt
    37801 gtccgtatcg ttacgccggg caccatcagc gatgaagcgc tgttgcagga acgtcaggac
    37861 aacctgctgg cggctatctg gcaggacagt aaagggttcg gctacgcaac gctggatatc
    37921 agttccggtc gttttcgcct gagcgaaccg gcggaccgcg aaacgatggc ggcagaactg
    37981 caacgtacga atccagcgga attactgtat gcggaagatt tcgccgagat gtcgctgatt
    38041 gaaggtcgcc gcggcctgcg tcgtcgcccg ctgtgggagt ttgaaatcga caccgctcgc
    38101 cagcaattga acctgcaatt tggcacccga gatctggtcg gttttggtgt ggagaacgca
    38161 ccacgcggac tttgtgctgc cggttgtctg ttgcagtatg cgaaagatac ccaacgcacg
    38221 accctgccgc atattcgttc tatcactatg gaacgtcagc aggacagcat cattatggat
    38281 gccgcgacgc gtcgtaacct ggaaattact cagaacctgg ccggcggtgc ggaaaatacg
    38341 ctggcttcag tgctcgactg cactgtaacg ccgatgggca gccgtatgct gaaacgctgg
    38401 ctgcatatgc cagtgcgcga tacccgcgtg ttgcttgagc gccagcaaac tattggcgca
    38461 ttgcaggatt tcaccgctga gctacagccg gtactgcgtc aggtcggcga cctggaacgt
    38521 attctggcgc gtctggcgtt gcgtaccgct cgcccacgcg atctggcccg tatgcgtcac
    38581 gctttccagc aactgccaga gttgcgtgcg cagttagaaa atgtcgatag tgcaccggta
    38641 caagcgctgc gtgagaagat gggcgagttt gccgagctgc gcgatctgct ggagcgagca
    38701 atcatcgaca caccaccggt gctggtacgc gacggtggtg ttatcgcatc gggctataac
    38761 gaagagctgg atgagtggcg ggcgctggct gacggcgcga ccgattatct ggagcgtctg
    38821 gaagtccgcg agcgtgaacg taccggcctg gacacgctga aagttggctt taacgcggtg
    38881 cacggctatt acattcaaat cagccgtggg caaagccatc tggcaccaat caactacatg
    38941 cgccgccaga cgctgaaaaa cgccgagcgt tacattattc cggagctgaa agagtacgaa
    39001 gataaagttc tcacctcaaa aggcaaagcg ctggcactgg aaaaacagct ttatgaagag
    39061 ctgttcgacc tgttgctgcc gcatctggaa gcgttgcaac agagcgcgag cgcgctggca
    39121 gaacttgatg tgctggttaa cctggcggag cgggcctata ccctgaacta cacctgcccg
    39181 acctttattg ataaacctgg cattcgtatt accgaaggtc gccatccagt ggttgaacaa
    39241 gtactgaatg agccgtttat cgctaacccg ctgaacctgt cgccgcagcg tcgcatgttg
    39301 atcattaccg gtccgaacat gggcggtaaa agtacctata tgcgccagac cgcgttgatt
    39361 gcgctgatgg cgtatatcgg cagctacgta ccggcgcaaa aagtcgagat tggcccgatt
    39421 gaccgtatct ttacccgcgt aggtgccgcg gatgatctgg cttccgggcg ttcaaccttt
    39481 atggtggaga tgaccgaaac cgccaatatt ttacataacg ccaccgaata tagtctggtg
    39541 ttgatggatg agatcgggcg tggaacgtct acttacgatg gtctgtcgct ggcgtgggca
    39601 tgtgcggaaa atctggcaaa taagatcaaa gcgttgacgc tgtttgctac ccactatttc
    39661 gagctgaccc agttaccgga gaaaatggaa ggcgtcgcca acgtgcatct cgatgcactg
    39721 gagcacggcg acaccattgc ctttatgcat agcgtgcagg atggcgcagc gagcaaaagc
    39781 tacggcctgg cggttgcagc tctggcgggt gtgccaaaag aggttattaa gcgcgcacgg
    39841 caaaaactgc gtgagctgga aagcatttcg ccgaacgccg ctgctacgca agtggatggt
    39901 acacaaatgt ctttgctgtc cgtaccggaa gaaacttcgc ctgcggtcga ggcactggaa
    39961 aacctcgacc cggattcact gactccgcgt caggcgctgg aatggattta tcgcttgaag
    40021 agtctggtgt aataataatt cccgatagtc ttttgctatc gggaatatta acaacaactg
    40081 acgaatcaaa taaaaatacc ctgtataata ggaaagctta ttttacaggg taaaaccatg
    40141 ccatctacac gctaccaaaa aatcaacgcc catcactatc gccatatatg gaccgttggt
    40201 gatattcatg gtgactatca attattacaa tcccgcttac atcaactctc tttttgccct
    40261 gaaaccgact tacttatttc ggtaggcgat aacatcgatc gtgggcccga gagtcttaac
    40321 gtgctgcgcc tgttaaacca gccctggttt atctccgtca aaggcaacca cgaagcgatg
    40381 gcgcttgatg cattcgaaac tggcgatgga aatatgtggc ttgccagcgg tggtgactgg
    40441 tttttcgatt taaatgattc agagcaacaa gaggcaatag atctgttgct gaaattccat
    40501 caccttccac atattattga aatcactaac gacaccataa aatatgtcat cgcacatgca
    40561 gattatccgg ggaaggagta tcagtttggc aaagaaatag cggagagcga attactctgg
    40621 cctgttgatc gtgtgcagaa atcacttaat agcgaattac aaaaaataaa cggtgccgac
    40681 ttctttatat ttggtcatat gatgtttgat aacattcaga cgttcgctaa ccagatttat
    40741 atagataccg gatcgccgaa aagcgggcgg ctgtcatttt ataaaataaa gtagtctcat
    40801 gcttcttctg tgaagcatga gtaacccggc gttattgcag gtcattattc atttttcgct
    40861 accagcaaag agagatccag cttcgccagc gcgcggctgg cactctccgg cagaccatcg
    40921 tcagtaatga tctgatcaaa ctcgcttaat ggtaacgcca gccatgtcgc cacctgtcca
    40981 tatttcgtcg catcacagac caaaactcgc tggcggctgg cactggcaat cgcccgtttc
    41041 accgtgactt tatcttctgc tggcgtagag atcccccgca cactccatga cgatgcagaa
    41101 ataaaagcct gatcaatcat caggctacgc agcatggtcg cagcggcttc cccaacacag
    41161 gaacggtttt cccgacacac tgcgccgcca gtgtgaataa ttgtgcaatt actgttgtcg
    41221 agcaagtagt ccgcaataac gaagtcgtta gtaaccactg tgagtggctc catgtgaatc
    41281 agatgctgtg caatcgctaa cgtggtcgtt cccgcatcca gatagataca acgtcccggc
    41341 tgaacaagac ttgccgccag cttgccaata gccgcttttt gcgtcattgc cagcgcagtt
    41401 tttacctgat gagaaggttc atgcgccacc cgtcccggag actggacgcc cccggacacc
    41461 agcacaacgg ctccctgctg ctccagtttt tgtaaatccc gacgaatggt catatgtgac
    41521 acattcattc tgtccgttag ttcagcaata ctaacaatgc ctttttcagc taccatctca
    41581 aggatgattt ggcgacgctc tacgggtatc aacttttgct ccttcctttg tcctgctgac
    41641 attctacgct atttgcctgc gaaacgtgtg cggcgcaact aacgcttagt tcacataaaa
    41701 taacacacaa tgttaatttt tgtgaatcag ctcaccatat cgttatcttt ccgcgcttat
    41761 attcacaaca tcaaacaaaa tatcacttaa attaacaagg agagcagatg aaaacgggat
    41821 cggagtttca tgtcggtatc gttggcttag ggtcaatggg aatgggagca gcactgtcat
    41881 gtgtccgcgc aggtctttct acctggggcg cagacctgaa cagcaatgcc tgcgctacgt
    41941 tgaaagaggc tggtgcttgc ggggtttctg ataacgccgc gacgtttgcc gaaaaactgg
    42001 acgctctgct ggtgctggtg gtcaatgcga cccaggttaa acaggtgctg tttggtgaaa
    42061 aaggcgttgc acaacatctg aaacccggta cggcagtaat ggtttcttcc actatcgcca
    42121 gtgctgatgc gcaagaaatt gctaccgctc tggctggatt cggtctggaa atgctggatg
    42181 cgccggtttc tggtggtgcg gtaaaagccg ctaacggtga aatgactgtc atggcctccg
    42241 gtagcgatat tgcctttgaa cgactggcac ccgtgctgga agccgttgcc ggaaaagttt
    42301 atcgcatcgg ttcagaaccg ggactgggtt cgaccgtaaa aattatccac cagttgttag
    42361 cgggcgtaca tattgctgcc ggcgccgaag cgatggcgct tgcagcccgt gcggggatcc
    42421 cgctggatgt gatgtatgac gtcgtgacca atgccgccgg aaattcatgg atgttcgaaa
    42481 accggatgcg tcatgtggtg gatggcgatt acaccccgca ttcagccgtc gatatttttg
    42541 ttaaggatct tggtctggtt gccgataccg ccaaagccct gcacttcccg ctgccattgg
    42601 cctcaacagc attgaatatg ttcaccagcg ccagtaacgc gggttacggg aaagaagacg
    42661 atagcgcagt tatcaagatt ttctctggca tcactctacc gggagcgaaa tcatgatcaa
    42721 gattggcgtt atcgccgatg attttaccgg cgcgacggat atcgccagtt ttctggtgga
    42781 aaacggtcta ccaacggtac aaattaacgg tgttccaaca ggtaaaatgc cggaagcaat
    42841 cgacgcactg gtgatcagcc tgaaaacgcg ctcctgtccg gtgcttgaag ccacacagca
    42901 atcgctggcg gctctgagct ggttgcaaca gcaaggttgc aaacagattt atttcaaata
    42961 ctgctctact ttcgacagta cggcgaaagg taatatcggc ccggttaccg atgcattaat
    43021 ggatgctctc gacacaccgt ttacggtctt ctctccggcc ctgccggtca acggacgtac
    43081 ggtttatcag gggtatttgt tcgtaatgaa tcaactgctg gccgaatccg ggatgcgcca
    43141 tcacccggta aatcccatga ccgacagcta tcttccccgt ctggttgaat cgcaatccac
    43201 agggcgctgc ggcgtcgttt cagcacatgt tttcgaacaa ggtgtgaaag ccgttcgtca
    43261 ggagctggct cgcttacagc aagagggcta tcgctacgcg gtgcttgatg cgctgaccga
    43321 acaccatctg gaaattcagg gggaagcctt gcgcgatgcc ccactggtaa cgggcggttc
    43381 tggtctggcg attggccttg cccggcagtg ggcgcaagaa aacggtaacc aggctcgcaa
    43441 agcagggcat ccgctcgctg ggcgcggcgt agtgctctcc ggttcatgct ctcaaatgac
    43501 caaccgccag gtagcacatt accgtcaaat tgcaccagcc cgtgaagttg atgtggcacg
    43561 ctgcctctca attgaaactc tggccgctta tgcacacgaa ctggcagagt gggttctggg
    43621 ccaggaaagt gtacttgctc cactggtttt tgccaccgca agcactgacg cattggcagc
    43681 aattcaacag caatacggtg cacaaaaagc cagtcaggca gtagaaacac tgttttctca
    43741 actagcggcg cggttagcag cggaaggcgt gacacgcttt attgtcgcag gcggtgagac
    43801 ctccggcgta gtcacacaga gcctgggcat aaaagggttt catattggcc caaccatttc
    43861 ccctggcgtg ccgtgggtaa acgcactgga taagcctgtc tcactcgccc ttaaatctgg
    43921 caacttcggt gatgaagcct ttttttcacg agcccaaaga gagtttttat catgagcgat
    43981 ttcgcaaaag tagagcagtc tttgcgagag gagatgacgc ggattgccag ttcattcttt
    44041 cagcgcggct atgcaaccgg ttcggctggc aatctgtcgc tgcttttacc tgacgggaat
    44101 ttactggcga caccgacagg ttcatgcctg ggcaatctcg atccgcagcg gctttccaaa
    44161 gtcgccgcgg atggcgaatg gttaagtggt gacaaaccct cgaaagaggt gctctttcat
    44221 ctggcgctgt atcgcaacaa tccgcgctgt aaagcggtgg tgcatttgca cagcacatgg
    44281 tcgacggcgc tttcctgcct gcaagggctg gacagcagca acgttattcg tccgttcaca
    44341 ccatacgtgg tgatgcggat gggaaatatc ccgctggtgc cttattaccg accgggcgat
    44401 aaacgcatcg cacaggatct ggcggaactg gcagcagaca atcaggcttt tttactggca
    44461 aatcatggcc cagtggtttg cggtgaaagc ctgcaagaag ccgccaacaa tatggaagag
    44521 ctggaggaaa cggcaaagct gatttttatt ctcggtgacc gcccgatccg ttatctgacc
    44581 gcaggtgaaa ttgcggaatt aaggagttaa tgcaatgcct cgttttgcag ctaatttatc
    44641 catgatgttc accgaagtgc cttttattga acgcttcgcc gcagcgcgaa aagccgggtt
    44701 cgatgctgtg gagtttctgt ttccctatga ctactccccc cagcaaatcc agcagcaact
    44761 ggtgcaaaac catctgacac tggcgctgtt caataccgct cccggagata ttaatgcggg
    44821 agaatgggga ttatccgccc ttcccggacg tgagcacgaa gcacgcgcag acatcgacct
    44881 ggcactggaa tatgcattgg cgctcaattg tgaacaagtc catgtgatgg caggcgtcgt
    44941 gcccgctggc gaagatgctg aacggtaccg ggcagtattt attgataatc tccgctacgc
    45001 tgccgaccgc tttgcgccac acggcaagcg aatattggtt gaagcactca gccccggcgt
    45061 taagcctcat tatctcttct ccagccagta tcaggcactg gctattgttg aagaggttgc
    45121 gcgagataac gtgtttattc aactagatac tttccatgcg caaaaagtag atggcaacct
    45181 gacgcattta atccgcgact acgccgggaa atatgcgcac gtacaaattg ccggactacc
    45241 tgatcgacat gaaccggacg atggagaaat caactatccg tggctgttcc gcctgttcga
    45301 tgaagtggga tatcagggct ggatcggttg tgaatacaaa cctcgtggcc tcaccgaaga
    45361 agggcttggc tggtttgacg cctggcgcta attcgctcaa tccctaatta caacgtaccc
    45421 atacatcccc ccatctacgg atggggtagg ttaactataa ctttcagaca gggtttccca
    45481 tgtccacaat tacattgtta tgcattgcgt tagctggcgt aatcatgctg ttgctactgg
    45541 tcatcaaggc aaaggtacaa ccattcgttg ctctgctcct cgtcagcctg ttagtcgcac
    45601 ttgcggcggg tataccggcg ggcgaagtgg gtaaagtgat gatcgccggg atgggcggtg
    45661 tgcttggttc cgtcactatt attattggtc tgggcgctat gctggggcgt atgatcgaac
    45721 actctggtgg tgcagagtca ctggctaatt atttcagtcg caagttaggt gacaaacgaa
    45781 ctatcgctgc gctgactctg gcagcgttct tcctcggtat tcccgtcttc tttgatgtcg
    45841 gctttattat tcttgcgcca atcatttacg gttttgccaa ggttgccaaa atatcaccac
    45901 tcaaatttgg cctgcctgtc gctgggatca tgctcactgt tcacgtggcg gtaccgccgc
    45961 atccaggccc tgtcgccgca gcggggttac tccacgcaga catcggctgg ctaaccatca
    46021 tcggtattgc gatttctatt cccgtaggga ttgttggcta ctttgcagcg aaaataatca
    46081 ataagcgcca atatgcgatg tcggtcgagg tactggaaca gatgcaactg gctccggcca
    46141 gtgaggaagg cgcgacaaaa ttaagcgata aaataaatcc accgggcgtc gcgctggtca
    46201 cctcgctaat tgttattcct atcgcgatta tcatggcggg tacggtttct gcaacactga
    46261 tgccgccttc gcatcccctg cttggtacgc tacagttgat cggctcacca atggtagctc
    46321 taatgattgc gctggtgctg gcattctggt tattggcttt acgtcgcggc tggagcttac
    46381 aacacaccag cgacattatg ggctctgccc ttcccactgc ggcggtagtg attttggtta
    46441 ctggtgctgg aggggtattt ggcaaagtgc tggtggaatc gggcgtcggt aaagcccttg
    46501 ccaacatgtt acaaatgatt gacctgcctc tgttaccagc cgcatttatt atttcactgg
    46561 cgctgcgtgc atcgcagggg tcagcaaccg tagcaatcct gacaaccggc gggttactct
    46621 cagaagcggt gatgggatta aatccgattc agtgcgtatt ggtgacgctg gcagcctgct
    46681 ttggtgggct tggcgcgtcg catattaatg actcagggtt ctggattgtg accaaatatc
    46741 tggggttgtc ggtagcagac ggtctgaaaa cctggacagt gttaacgacc attctcggtt
    46801 ttaccggatt cttaattacc tggtgcgtat ggctcgtcat ctaataatcc agataagata
    46861 tgatgtatcg tttaagtgcc tgataagggt gtaatgtatg ccaatattaa cctcggtaat
    46921 atctcgctac cgaggttaat tttcactttg caatataatt acagtctctt gcaagttgat
    46981 tttatcttag taataagaac cgactaatct tattgccatt tcgatattag caggcataga
    47041 tgcgtcgtag ccatacatat ggccatattt cggacgtaat acttcagtta ctttcttaat
    47101 aattgcttcg cgctgggccc gcgcaaaatc agcatgatta tttgccgttt tattcataaa
    47161 tttaccaaaa ctttcttctt ttctgacttt aattgtcgta tccctaagat cattaagaaa
    47221 ctggcgttga aatttgaaat catcgtgtga accaatatta ccatgcccgc caataaaata
    47281 tttccagtca agcatttcaa cctgacgcaa attactgtca tggtaaacca acgtatccga
    47341 tacggcgaag cccatcatcg gcagttggtc aggattcagg agatccgggc tgtgagctac
    47401 tttttcctgt tttagcaacc atacagaatg gtcgtcggta tgtcctgctt taacaaatcg
    47461 gtgtaattca atggtctgct tttcaaattt aaactggtca tcttttttac ttaaaacctg
    47521 agtggcgacg ggtaagcgac tgttcataaa ttgcattttt tcagcagtgg ctttactggc
    47581 aataatacgc agattcacgc cttctttctt cgcctcatcg ttccaaaatg gactatcgcc
    47641 aatgtgatcc acatggtaat gagaataaac cagcgctgtt acgggtaatg gagtcacatc
    47701 acgaattgcc tgtaataaat atttaccgcg accctccgga gcatcaaaca gcaaaacccc
    47761 tttgtctccg acataaaacg tagtggaata aaaatagcgt tgaacataat aagttctctc
    47821 gccaattttt tgtaaaatat aaggctgaga catatttctt tcaaaaaggt tattgagttt
    47881 atcgcttggc gatattattt ttttcactga aggttcaaca taacttgcat taactgtaga
    47941 cgaatttgca gtcgaaattt ccgtttgagc aaaaatacta ccagatataa gagaagaggc
    48001 tagaattgta tatattttaa tgccttgcat acgtagtcct ttattaatgt gttgttgatt
    48061 tatagcatct ctgaacagac tgttatttta gatattacta cgttacgaga ttcatttttc
    48121 ttcgaatatt atgtttatct cattcaaaaa atatgttcat catttgatgg tttaaataat
    48181 atctgcaagg cataatactc cacatcttta aaacctgcct caaaaaatca ttcattagta
    48241 aaaaggatat ttatatgcaa aacatagctg tggtttatta ttcaggatat ggtcacacta
    48301 aactggttgc tgaaaaagtg gcacaaggcg cccatgcaga tttaatttcg attgataacg
    48361 aaggaaatat cactgagcag gagtgggaaa aactggacgc agccgatgcc attatcttcg
    48421 gtgctcccac ctatatgggc ggtgcgccat ggcaattcaa gaaatttgct gatgccagtt
    48481 ccaaagcgtg gtttacacgt aaatggcagg ataaagtctt cgggggtttc accaacagcg
    48541 ccagcctgaa tggcgataaa caagttacgc tgatctatct gcaaacgctg gcttcacagc
    48601 atggcggtct gtgggggagt ttaggtcagg ctcctgccaa tgtgcttgcg tcaaccaggg
    48661 aagatgtaaa caatctgggc ggttcagtcg gtttgctggt gcaatctcca gccgatgcgg
    48721 gtgcagacca aatccctacg ggcgatctgg atacagccgt gaaatatggt gaacgcgtgg
    48781 cgacaattac tgcgcgctta aaatgatgat tgccgaatgt gacgctgacg cgtctatccg
    48841 gtcttctaca tctaaaaagt gccgtcccct ggtcagtcag ataaagtttt aacgctgtat
    48901 ccgataacag ttcaacatgt accaaaagaa aaaaagccag cctcgcttga gactggcctt
    48961 tctgacagat gcttacttac tcgcggaaca gcgcttcgat attcagcccc tgcgtttgca
    49021 ggatttcgcg caaacggcgc aggccttcaa cctgaatctg gcgaacacgt tcacgggtga
    49081 ggccaatttc acgacctaca tcttccagtg ttgccgcttc gtaccccagc aaaccgaatc
    49141 gacgtgccag tacttcacgc tgtttggcgt tcagctcgaa cagccatttg acgatgctct
    49201 gcttcatatc gtcatcttgc gtggtatctt ccggaccatt ctctttttca tcggccagga
    49261 tgtccagcaa cgctttttcg gaatcaccac ccagcggggt gtctaccgag gtaatgcgct
    49321 cgttaagacg aagcatacgg ctgacgtcat caactggctt atccagttgc tctgcgatct
    49381 cttccgcact tggttcgtgg tccagcttat gggacaactc acgtgcggtt cgcaggtaaa
    49441 cgttcagctc ctttacgatg tgaatcggca aacgaatagt acgggtttgg ttcataatcg
    49501 cccgttcgat cgtctggcga atccaccagg ttgcgtatgt tgagaagcgg aaaccacgtt
    49561 ccgggtcaaa cttctctacc gcgcggatca gccccaggtt gcccacttcg atcaggtcca
    49621 gcaacgccag accacgattg ccataacggc gggcaatttt taccaccaga cgcaagttac
    49681 tctcgatcat ccggcggcga gaggcgacat ctccacgcag tgcgcgacgc gcaaaataaa
    49741 cttcttcttc ggccgttaac agtggtgaat aaccaatctc accaaggtaa agctgagtcg
    49801 cgtccaacac acgctgtgtg gctccctgcg ataacagttc ctcttcggcc aaatcgttat
    49861 cactgggttc ctcttctact aaggcctttt cgtcaaaaac ctcaactccg ttctcatcaa
    49921 attccgcatc ttcatttaaa tcatgaactt tcagcgtatt ctgactcata aggtggctcc
    49981 tacccgtgat cccttgacgg aacattcaag caaaagcctg gttccgccga tttatcgctg
    50041 cggcaaataa cgcagcgggt ttacggattt ccccttgtaa cgaatttcaa aatgcaagcg
    50101 tgttgaactg gttccggtgc tacccatggt tgctattttt tgccccgcct tcacttcttg
    50161 ttgttcccgg accagcattg tgtcgttatg ggcgtaggca ctcaggtaat catcattatg
    50221 tttgatgata atcagattac cgtagccgcg cagcgcgtta ccggcataaa caacgcggcc
    50281 atccgcggtc gcgataattg cctgtccttt gctgcctgcg atatcaatcc ccttgttgcc
    50341 cccctcagaa gcgccaaagg tttcgatcac tttgccctca gtcggccagc gccaggtgga
    50401 gataggcgta ctggttgatg tactgctgac agtcggctcg gttgtgcttg ctgttggtac
    50461 cgttacaggc gctgtgaccg tggtcgcagt tggcttgttg ttcggcaaca ttttgttagc
    50521 actctgttca cccgaagact cagaatacgt aattgtcggt tgcgacgcaa cagcaacggt
    50581 ggaattttgt gcaggcttga tcacaactcc ttgctctgct gcgtcggcct gggtaatggc
    50641 atttccgcct gtgattggcg taccggaagc attacccacc tgtaaggtct gaccgacgtt
    50701 cagcgcgtat ggtgcctgaa tattgttgcg ctgagcaagg tcacggaaat cgttgccagt
    50761 aatccaggcg atatagaaaa gcgtgtcgcc ttttttcacg gtgtatgtac tgccgctata
    50821 actgcctttc ggaatgttcc catactgacg gttgtagacg attcgtccgt tttccatctg
    50881 taccggctgc tgagctactg gctgcacagg ctggatttgc ggttgttgag tagcctgaat
    50941 ttgtggctgc tgtaccggct gaatttgcgg ttgctgcgct gtagacgtcg tccccatttt
    51001 cggcggcggc gtaatcaaca taccagaatt ggtatttgcg ggcgcattgc cattaacgga
    51061 gctgaccggt gccggtggat ttgaagtgtc agaacagcct gccagccata gcgaaaccag
    51121 tgacaatgcc gcaatgcggc gaacggtgaa ttttgggctt cccgcgctca tttatccccc
    51181 aggaaaaatt ggttaataac cagtgacata attaccgtgc aaggcaccat actgaacact
    51241 ggaaaagatg ttcacgatac gctgacttgc ggcaaaataa ccaggaaaaa tccaggtatt
    51301 tcctcacgtt ttaagccagc tcacccttca ctaaagggac aaagcgcacg gcctccacgg
    51361 tatcgataat aaattcgcct ccccgacgac gcacccgttt taaatactgg tgctcctccc
    51421 ctacgggtaa gacgagaatc ccgccttcgt ccagctgcgt cattagcgca gttggaattt
    51481 caggcggtgc cgccgtaaca atgatagcgt caaacggcgc gcgtgcctgc caaccttgcc
    51541 atccatcgcc atgacgggtt gaaacattat gtaaatcaag atttttcagg cggcgacgcg
    51601 cctgccactg caagccttta atccgttcaa ccgagcaaac atgttggaca agatgcgcca
    51661 ggattgcagt ttgatatccc gaaccagtgc caatttccag cacccgcgac tgcggcgtca
    51721 gctcgagtaa ttcggtcatt cgcgccacca tatacggctg cgaaattgtc tgcccctgac
    51781 ctatcggcaa ggcgatattg tcccaggctt tttgttcaaa cgcttcatca acgaattttt
    51841 cacgcggcac ggcggcaagt gcattcagca ccagctcatc ctgaatacct tgcgcacgta
    51901 attgatccag aagtgcttgt acgcgtctgc ttaccattgc gtgccaactc ccacgctgtt
    51961 taaccagtct gaaaccacat cttgcgcgct atgcgcagtt aaatccacat gcagcggcgt
    52021 gatggaaaca tagccctcat ctaccgcagc aaaatcggtc cccggaccgg catcacattt
    52081 accgcccggc gggccaatcc agtacagcgt attaccgcgc ggatcttgct gcgggatcac
    52141 ctgatctgcc ggatgtcgtg taccgcagcg cgtcacgcga atacctttga tttgatccaa
    52201 gggtaaatcc ggaacgttaa tattaagaat acgcccggtg cgcagcggct ctttacacag
    52261 tgcgcgcaaa attgagcagg ttaccgccgc ggcagtgtcg taatgtttat gcccgtcaag
    52321 cgagacggca agcgccggaa aacctaaatg acggccttcc atcgcggccg ctaccgtacc
    52381 ggaataaata acatcatccc ccagattcgg cccggcgtta attccggaca caacaatgtc
    52441 cgggtgcggg cgcatcagag catttacgcc aagatagacg caatcggtcg gggttcccat
    52501 ttgcacagca atatcaccat tttcaaaggt aaacgtgcgc agggaggatt ccagtgtcag
    52561 agaattagaa gcgccgctgc ggttacgatc gggggcgacc acctgaacgt cagcaaactc
    52621 acgcaaggct ttcgccagcg tttgtatacc gggtgcatgt accccgtcat cattactcag
    52681 caatatgcgc ataatcacct gttgtgttga taagttccct gacaacgctg gttgcaaaac
    52741 ttcccgccgg aagccagaaa cggatttcta cggtgacatc atcccaccag ttccagctta
    52801 attgttgcgg atacagcagc atcgctctgc gcgcggcttc aactttttcg cgcaccagta
    52861 aggtttgtaa ttcagtttct tcggcgacag ctgcttgttc gaatgccagc gcttcacgct
    52921 gagttcccca ctcgccactg cccggcaatg cggcggttat catcagcact ttatcgttga
    52981 cgcgacgctg taattccgcc agttcttcgg tggttgcgac aaaccagcta ccacgtccgg
    53041 ctaattgtag cgcatcgccg tcaacaactt gattaacgtc tgcttttttg aggcgttcag
    53101 caacaatctg attaaacaac gcactgcggg ctgccgacaa ccaaaaactc cgtttattgc
    53161 gatcgcgcac cggagtattg gtttgcgccc agcgcagcgc accctgcaag ttgctaccgc
    53221 caatcccaaa acgttgggca ccgaagtagt tcggtacacc ttttacgcaa atatcgatca
    53281 gacgttgttc aacgtcatcg cgattgctca cttcgcgtag taccagggta aaggcgttac
    53341 ctttcagcgc ccctaaacgc agcttgcgct tgtgccgcgc atactccagc acctggcagc
    53401 cttccagttg aaaggcgctc agatcgggca tctctttgcc cggcacgcga gcgcataacc
    53461 actgttccgt aacagcatgt ttatcttttt gcccagcgaa gctgacttca cgggcatgaa
    53521 ttttcaggaa tttcgccagt gcatccgcca caaaacgggt attgcagccg tttttgagga
    53581 ttctaaccag aatatgctca ccttcaccat caggctcaaa gcccaaatct tccaccacca
    53641 caaagtcttc cggattggct ttcagcagcc cggtgccttg cggtttaccg tggaggtaag
    53701 tgagattatc aaactcaatc attttgttgc cttaatgagt agcgccaccg cttcacaggc
    53761 aatcccttcc ccacgtccgg taaatcccag tttttccgta gtagtggctt tcacgttaac
    53821 atcatccata tggcagccga gatcttcggc aataaacacg cgcatttgtg gaatgtgcgg
    53881 caacatcttc ggtgcctgag cgatgatagt gacatcgacg ttgccgaggg cataaccttt
    53941 cgcctgaata cgacgccagg cttcgcgcaa cagctcgcgg ctgtcggcac ctttaaatgc
    54001 cggatcggta tccgggaaca gcttgcctat atctcccagc gccgccgcgc caagcaatgc
    54061 atcggtcaac gcatggagcg ccacgtcgcc atcagaatgc gccagcagcc ctttttcgta
    54121 aggaatgcgt acgccaccaa tgataattgg gccttcaccg ccaaaggcgt gtacgtcaaa
    54181 accgtgtcca attcgcatta tgtattctcc tgatggatgg ttcgggtgag gtaaaattcg
    54241 gccagcgcca aatcctccgg gcgcgttacc ttgatgttat ccgcgcggcc ttcaacaagt
    54301 tgcggatgaa aaccgcaata ttccagcgcc gaggcctcat cggtaatggt agcgccttca
    54361 tttagagcgc gcgtcaggca gtcatgtaac agctcacgag ggaaaaattg cggcgtcagc
    54421 gcgtgccata agccgttgcg atcaacggta tgagcaatgg catttttgcc cggttcggca
    54481 cgtttcatcg tatcgcgcac cggcgcagcg aggatccctc ccgtgcggct ggtttcgctc
    54541 aacgccaaca atcgcgcgag gtcatcctga tgcagacaag gacgagcggc gtcatgcacc
    54601 aatacccact gcgcgtcgcc agcggctttc agacctgcca gcacggaatc ggcacgctct
    54661 tcaccaccat caacaaccgt gattcgtgga tgattcgcca gaggaagttg tgcaaagcgg
    54721 ctatcgccag gacttatggc aatgacgaca cgtttcaccc tgggatgcgc cagcagcgca
    54781 tgcaccgagt gttcaagaat ggtttgatta ccgattgaga gatattgctt aggacattcc
    54841 gtttgcattc ggcggccaaa tccggccgcc ggaaccacgg cgcaaacatc caaatgagtg
    54901 gttgccatgt taattcccgg gctgatttat cgattatttt gccccgcaga ctgtgcgcgc
    54961 tttgacgcgt caggcaccag acgataaaac gtttcgcccg gcctggtcat gctgagttca
    55021 ttacgcgcac gctcttcgag cgcctcctgg ccgccattga gatcgtcaat ttcggcaaaa
    55081 agctgatcgt ttcgcgcttt aagtttcgcg tttgtagctt gctgtgccgc cacatcatta
    55141 ttgacgcgag tatagtcatg tataccgttc ttaccgaacc acagcgaata ctgtagccag
    55201 accagaatag ccagcaacag cagcgttagt ttacccatcc tgccccctga aaaacggcat
    55261 cttcatccca tgcatccgaa gacgactcta catcctctgt tggggatacc gcgacaacgc
    55321 gggcaaatgt accacatttg tcgattgtta cgtataccca gggcgtgcag aacataatct
    55381 cattattagt tacggattga attatgaaca gaatagagag gaaagaacaa ttaacccagt
    55441 agccacataa acagtgcacc aaacataatg ccaaccgtcg ccagagtgaa aacaatactg
    55501 tagcgtagct ttccgtccat caatgaatgc agtgcaatcc ccaccactac agcgacgggc
    55561 atcagcgcca gaaagaaagg ccaggtgtag ataaaaaaga gcagcgtgtt agagccataa
    55621 atcaacatcg gaattgccaa cgcgcacagc cacgataaaa aaccaaccac agcgccaggc
    55681 agcgaccatg tggtgtcttc aacctcagta aggctgtcgt tatttattaa tgtaatgttg
    55741 tggctgttac gcatatctga tcctgttact ttgacgaacc gggcatgaaa acccggtggt
    55801 gtctcaggat ctgataatat cgttctgtct caacagatct aataattgct gtaccaaatt
    55861 tgttactaat tgttcaccat tgagatgaat ttctgccgat tcaggcgctt cgtaaacgga
    55921 atctattccc gtaaagttgc gcagttcacc ggcacgcgct ttcttatata aaccttttgg
    55981 atcgcgggct tcgcaaatcg ccagcggcgt atcgacaaac acttcgataa agcgcccttc
    56041 tcctacgcgt tcgcgaacca tctggcgttc ggcgcggtgt ggcgagataa atgcggtcag
    56101 caccaccagt ccggcttcaa ccatcaaatt cgccacttca ccgacgcgac ggatattctc
    56161 tttacggtcg gcatcgctaa aaccgagatc gctgcataat ccgtggcgaa cgttgtcgcc
    56221 atccagcaga tacgtactga cgccaagttt atgtaacgcc tcctccagcg cccccgcgac
    56281 ggttgattta ccggaaccag agaggccggt aaaccacagc acaacaccac gatgaccgtg
    56341 gtgctgttcg cgttgttgcg gagttactgg gtggctatgc cagacgacgt tttcgtcatg
    56401 cagagccatt atttatctcc cagtaaatcg cgcgcacccc aatgcgggaa atggcggcga
    56461 accagggcat tcaattccag ttcgaatgca ctgaattcag atggcgcagc agttgcctgg
    56521 ctaactggtt cgtgcaccat accggcacct acggtcacat tactcaggcg atcgataaaa
    56581 atcagcccac cggtaaccgg gttttgctga taacgatcta acaccagtgg ctcgtcaaag
    56641 gtgagatcta ccaggccgat gccgttcagc ggcaggtttt caacttcgcg ttgggtaagg
    56701 ttattgatat caacctgata atgaatgcca tcaacacgag cacgcgtctt cttaccggca
    56761 attttgatgt cgtaactctg gcccggggaa agcggctgtt ccgccatcca taccacatcc
    56821 accgacgcgc tctgcacagc cggtaacgct tcgtctgccg ccagcagcag atcgccacgg
    56881 ctgatgtcga tctcatccgt cagcaccagg gtgatcgctt ctccggcaaa ggcttcttcg
    56941 cgatcgccat caaaggtcac gatccgcgcg acgtttgatt ccacaccaga gggcagcact
    57001 ttgacacgtt gcccgacttc cacacgaccg gatgccagcg ttccggcgta gccacgaaaa
    57061 tcgagattcg gacggttaac gtactgcacc gggaagcgca ttggctgagc atccaccact
    57121 cgctggatct ccacggtttc cagcacttct agcagtgtcg gaccgctgta ccacggcata
    57181 ctttcacttt gcgaagccac gttgtcgcct tccagtgcgg agagcggcac aaagcggata
    57241 tccagattac ccggcagctg cccggcaaaa gtcagataat cttcacgaat acgggtgaac
    57301 gtctcttcac tgtaatccac cagatccatt ttgttgatcg ccacgaccag atgtttgatc
    57361 cccaacagtg tggagataaa actgtgacga cgggtttgat cgagcacgcc tttacgggca
    57421 tcgatcagta agatcgccag ttcacatgtc gatgcgccag tcgccatatt gcgggtgtac
    57481 tgctcgtgcc ctggggtgtc ggcgataata aatttacgct tctcggtaga gaaatagcgg
    57541 taggccacat caatagtgat gccctgttcg cgctcagctt gcaggccgtc caccagcagt
    57601 gccagatcca gcttttcgcc ctgggtgccg tgacgcttac tgtcgttatg cagcgatgag
    57661 agctgatctt cgtagatttg gcgggtatcg tgcagcaggc gaccaatcag ggtacttttg
    57721 ccgtcatcga cgctaccaca ggtcagaaaa cgcagcaggc ttttatgttg ttgcgcaatc
    57781 atccaggctt cgacgccgcc ttcattggcg atttgttgtg caagtgcggt gttcatctta
    57841 aaaatacccc tgacgttttt tcagctccat agaccccgcc tggtcgcggt caattacgcg
    57901 gccctgacgt tcactggtgg tggaaaccag catctcttcg atgatctccg gcagtgtttg
    57961 cgcatttgac tccaccgcgc cggtcagcgg ccagcagccc agcgtacgga aacgcaccat
    58021 ccgtttttta atcacttcgc ccggttgcag gtcgatacgg ttgtcatcaa tcatcatcaa
    58081 cataccgtcg cgttccagaa ccggacgttc cgcagcaaga tacagcggaa caatgtcgat
    58141 attttccagc cagatgtatt gccagatatc ctgctcggtc cagttagaga gcgggaagac
    58201 gcggatgctt tcgcctttgt taatttgccc gttgtagttg tgccacagct ccgggcgctg
    58261 atttttcgga tcccagcgat ggaagcggtc acggaaagag tagattcgct ctttagcgcg
    58321 ggatttctct tcgtcacggc gcgcaccacc gaaggcggca tcaaaaccgt atttgttcag
    58381 cgcctgtttc aggccttcgg ttttcataat atcggtatgt ttcgcgctgc cgtgtacgaa
    58441 tggattaatc cccatcgcca cgccttccgg gtttttatgc accagcagct cgcagccgta
    58501 ggctttcgcc gtacgatcgc ggaactcata catctcgcgg aatttccagc cggtatcgac
    58561 atgcagcaac gggaaaggca gcgtacctgg ataaaacgcc ttgcgcgcca gatgcagcat
    58621 gacgctggaa tctttaccga tagagtagag catcaccgga tttgagaatt ctgcagccac
    58681 ctcgcgaata atgtggatgc tttccgcctc cagttgccgc aagtgggtaa gtcgtttttg
    58741 atccataacc gttcctttgc aataccgcta ttttcttgcc atcagatgtt tcgactatag
    58801 ggagcgtaag agaacgaatg aaattaccaa ttagaatgag tagttcctta acggaataac
    58861 gatttggcaa agctaatatc aaaaagtgct taaggcaccg gatttcgggc gtttaggaag
    58921 atttgaaatt gttttagcgc agcggcagtt tcatactatg gcggtaaaaa aatttgcatg
    58981 gtatttaagg actcactatg ttttccgcat tgcgccaccg taccgctgcc ctggcgctcg
    59041 gcgtatgctt tattctcccc gtacacgcct cgtcacctaa acctggcgat tttgctaata
    59101 cccaggcacg acatattgct actttctttc cgggacgcat gaccggaact cctgcagaaa
    59161 tgttatctgc cgattatatt cgccaacagt ttcagcaaat gggttatcgc agtgatattc
    59221 ggacatttaa tagtcggtat atttataccg cccgcgataa tcgcaagagc tggcataacg
    59281 tgacgggaag tacggtgatt gccgctcatg aaggcaaagc gccgcagcag atcatcatta
    59341 tggcgcatct ggatacttac gccccgctga gcgatgctga cgccgatgcc aatctcggcg
    59401 ggctaacgtt acaaggaatg gatgataacg ccgcaggttt aggtgtcatg ctggaactgg
    59461 ctgaatgcct gaaaaatacg cctaccgagt atggtattcg atttgtggcg accagcggcg
    59521 aagaggaagg aaaattaggc gctgagaatt tactcaagcg gatgagtgac accgaaaaga
    59581 aaaatacgct gctggtgatc aatctcgata acttaattgt tggcgataag ctgtatttca
    59641 acagtggcgt aaaaacacct gaagcagtaa ggaaattaac gcgcgaccgg gcgctggcaa
    59701 ttgcccgcag ccatggaatt gccgccacca caaatccagg tttgaataaa aactatccga
    59761 aaggcaccgg gtgttgtaat gacgcagaaa tattcgacaa agccggtatt gctgtacttt
    59821 cggtggaagc gactaactgg aatcttggca ataaggatgg ttatcagcaa cgcgcaaaaa
    59881 cagccgcatt ccctgcagga aatagctggc atgacgtaag actggataat caacagcata
    59941 ttgataaggc acttcctgga agaatagaac gccgctgccg tgatgttatg cggataatgc
    60001 taccgttggt gaaggagctg gcgaaggcca tcttaatggg tttgaaatga ttactccctt
    60061 gcctgcagga aatcaatgcc accagaaaca gagtataaaa aagggtgcag gagaccaaac
    60121 catcacaatt cagtacgcgg ggttttaccc cgcgtggtgg ctacttcttc gattcgtaag
    60181 cgagaactgc cttaaactcc ataccacgtt ctctgatact caactcacac aacgagtcac
    60241 ctaccatcaa cgtgaatccc agtactgtac aacacagtac gatgattgcc acaagggcat
    60301 attttgtcag catgtcttgc ctccctgcaa agaagagact aacatccgaa ttgctacgtt
    60361 cgaacgcgag cctcaggttg atagaaatat cgcctggggc ttttgtccat ctggaacctc
    60421 gcgaatgctt aacgccagac agcctcaagc acccaatgcc attctatacc tgttaattct
    60481 ccctgcgacg ttctcgttta aatgccggaa tgctgctcgc aaaataaaaa cgccccatca
    60541 tttctgacag aggcgtttaa tttatccggc aatatttacc cttcgtgtaa tccacattcc
    60601 ctttttaagc caaagaaacg cgtttcttct tccgctatgc cgggttccca tttacgggtt
    60661 gtatgggtat cgcccaccga taaatatcct tcatcccata atgggtgata tttcaggcca
    60721 tgtttttgca ggtactgata aatagttcgg ttatcccagt cgataatcgg cagcacttta
    60781 aatacgccac gctgaattgc cagcaccggt aaattggcgc gactgccgga ttgttcacgg
    60841 cgcaggccag caaaccaggt ttgcgcattc agttctttca gcgcccggtt catcggttcg
    60901 actttgttga tgtcattgta cttttcaatg ccttcaacgc cctgctccca cagtttgccg
    60961 tagcgtgctt cctgccaggc ggcgctttcg gtagcacggt acactttcag gttgagcttg
    61021 agtttgtccg ttaactcgtc aataaagcgg taggtttccg ggaacagata gccggtatcg
    61081 gtgaggatca ctggaatatc cgggcgaatc tgattcacca gatgcagact caccgccgcc
    61141 tgaatgccaa agctggaaga aagtacatat tcaccgggca gattatccag cgcccaggct
    61201 acgcggcctt cagcgtccag tttttccagt tgggcgttag tttccgccag cgccagaatc
    61261 cggtctacct tcggcagttc gttcagggcg tttagatcga gtttggacat aagttcctca
    61321 ctgtttgcct tgcctgatgc aacgcttaca catcttatca ggcctacaag accgggctga
    61381 tggttaatcc cacaaatcgc gcgccggatc gagcaccggg cgaatgatgc ccgcacgcac
    61441 cgtaaagtcg ccgaagcctt cacccacttc gcgctctttc gcccagcgcc ctatcagttc
    61501 atcaagcgac gccaggattt ccggctcggt gatgttttct ttatacatcc gtgggatacg
    61561 tgtcccaatg cggttgccgc caagatgcag gttgtagcga cccggcgctt tccccaccag
    61621 acccacttcc gccagcatcg cgcgaccaca accgttcggg cagcctgtta cacgcatcac
    61681 gatatgctca tcgctgacac catgtttcgc cattaaatta tcgatgttgt cgataaaaga
    61741 cggcaggaaa cgctccgctt ccgccatcgc cagcgggcaa gtcgggaatg acacgcaggc
    61801 cattgagttt tcacgctgcg gcgtgacggc attcattaac ccgctttctt tggcgatctt
    61861 ctcgatcttc gctttctcgc tttccggcac gccggcgatg atcagattct ggttcgccgt
    61921 aatgcggaaa tcacctttgt ggatcttcgc gatctccagc aagccggttt tcagcggacg
    61981 tcccggataa tcaagaatgc gaccattttc gataaacagc gtcaggtgcc agttatcatc
    62041 aatgccctta acccagccaa tacgatctcc gcgtccggta aactcatacg ggcgaatcgg
    62101 ctcgaatttg atccccgcgc gacgttcaac ttccgcttta aacgtctcaa cccccacgcg
    62161 ctccagcgtg tatttggttt tggcattttt acgatcggtt cggttacccc agtcacgctg
    62221 agttgtcacg acggcttccg ccaccgccag cgtatgctcc agcggcagat agccaaactc
    62281 actcgccgtg cgggcgtagg ttttcttgtt gccgtgttcg atggaaagcc caccgcccac
    62341 cagcaggtta aagcccacca gcttgccgtt ttcggcgatc gccacgaagt tcatgtcgtt
    62401 ggcgtgcaga tcgatatcgt tctgcggcgg gatcactacc gtggttttga acttacgcgg
    62461 caggtaggtc tgaccaagga tcggctcttc atcagtagtg gcgacttttt cctgatcgag
    62521 ccagatctcc gcatacgcgc gggtacgagg cagcagatgt tcagaaattt tcttcgccca
    62581 ttcatatgct tccgcatgca gctgcgactc gtaagggttc gaggtgcaga gtacgttacg
    62641 gttcatgtcg ttggcggtcg ccagcgcatc aagaccgacc gagtgcagca tctggtgcac
    62701 cggtttgacg ttctttttca gaatgccgtg gaactggaat gtctggcggt tggtcaggcg
    62761 aatgctgcca tagatggtgt tttcaccggc aaatttgtcg atcgcctgcc actgtttagt
    62821 ggtaatcacc ccacccggca gacgacagcg aagcagcatc gcgtggcgcg gctccagctt
    62881 ctgttcagca cgttcggcgc gaatgtcgcg gtcatcctgc tgatacatgc cgtggaagcg
    62941 gatcagcagg aagttatcgc ccttaaagcc gccggtcaga ccgtcgttta aatcttccgc
    63001 aatggtgccg cgcaggtagt tgctttcaag cttcatgcgc tcggcgtctg tcagttttcc
    63061 ttcgaccact aaaggccctg gatgtttttc actcattagt agacatctcg ctgataacgg
    63121 cgctctacgc gcagctcact taaaaattca tccgccgctt cggtgtccat gccaccaaat
    63181 tcggcaatca cttccagaag tgcctgctca acgtctttcg ccatgcgatt agcgtcgccg
    63241 cagacataaa tgtgggcacc gtcattgatc cagcgccaca gctctgcgcc ctgttcgcgc
    63301 agtttgtctt gtacgtaaat tttttctttt tgatcgcgcg accaggcgag atcgatgcgc
    63361 gtcagcacgc cttctttgac gtaacgctgc cattccacct ggtagaggaa atcttccgta
    63421 aagtgtgggt tgccaaagaa tagccagttt ttacccggcg cttcgtcggc agcacgctgc
    63481 tgcataaagg cacggaacgg cgcgatgccg gtgccagggc caatcatgat caccggggtt
    63541 tccggattgg ttggcaggcg gaagttgtcg ttatgttcga taaagacgcg gacttcaccc
    63601 tcttcttcca cacgatccgc gaggaagctg gaggcaccac cggcacgggc gcggccttcc
    63661 acgtcgtaac gcaccacacc aacggtaacg tgcacttcgt tctcgacttc cgcctgcgag
    63721 gaggcgatgg aatacaggcg cggggtcagc gggcgcagca gattaatcag cgcttcggca
    63781 tccagttgcg ccggagagaa acgcaccatg tcgacaatcg gcgtcgtcgc ggcgtaatgc
    63841 tgtaacttcg ctttatcgcc caccagcggc agcagcgttt cgctgcgggt aagcgtggca
    63901 tagttctcaa caatgttggc ggtgttgacg gtcagttcga agtgccactg tagcgcttcg
    63961 ttcagaggca acgttttgcc ctcgacggtg acaggttcat cgcctttcag ccacagcagt
    64021 tcgacaagtt ctttcaccag tgccggatcg ttctgatacc agacgcccaa cgcgtcaccc
    64081 ggctggtaac gcaggccaga gtcacctaag tcaatttcaa tgtgacgaac gtctttttca
    64141 gagttacgcc cggtaatttt ctggttaacc gaaagactgg ccgccagcgg cgcgtctttg
    64201 ctatacgggc tggtgtggat ttcatttacc gtgccagtag cgacggattg cgaaggtgcc
    64261 gcgacaggcg cacgcgattt aagcgcatca accacgcggg cgcgccactc gctggcagca
    64321 gcctggtatt caacatcggc atcgacacgg tctagcaggc gttcaccgcc cagttccgcc
    64381 agcttactgt caaaatcttt cccggactgg cagaaaaatt cataagagct atcgccgagg
    64441 ctaaacacgg caaacgcggt gttttccagc tttggcgctt ttttggagaa caggaactta
    64501 tgcagcgcga cggcttcttc cggcggttcc ccttcccctt gcgttgacgt caccacgata
    64561 agcagttttt cgctggcgat ttgtttgaat ttatagtcac ccgcgttcac cagcttaacg
    64621 ttcagttttg ccgctaataa atcatcgcgt aatgcttcag caacccggcg cgcattgccg
    64681 gtttgcgagg cggagataat agttataccc ggcatttctg cggcgggcgc tggcgtcgct
    64741 gcaagcgcag caggctgctg gttgagcacg ccccagaaat agccagaaac ccaggcaagc
    64801 tgggtaggag ttaaatcggt cgtggccgcc tgaaggcgtg ccagttgctc cgggttcaac
    64861 ggaagcaacg cggaaggtgg gacctgtgtc gtcatgcgtt gttatgttcc agtaagcaaa
    64921 gctgtttctg cgccctgtca gcgcccataa aacagaagag aaggtaaggt taacggggta
    64981 aacggtgtgg attaaagacg ggatagcgat aactaataac caaatcgact aacctgtttt
    65041 agcaatagtc tttaacaaca aaatagatta accaacccaa tgaaaaacaa atgaatattg
    65101 cccatcaata agataaatca gcgattttgc gcaacaaagt cgttttagat aatgcgaaaa
    65161 aacagccttt ccggtactct acggcggttt tatcgtcatg tagagaaatt atgatgtcca
    65221 ccacgttatt taaagatttc accttcgaag ccgctcaccg cttgccgcac gtcccacaag
    65281 ggcataaatg tggtcgcctg cacgggcatt cctttatggt gcgactggaa attaccgggg
    65341 aagtcgatcc gcatacgggc tggattatcg atttcgctga actgaaagcg gcgtttaaac
    65401 caacttatga gcgcctcgat caccattatc tcaatgatat tccaggtctg gaaaacccaa
    65461 ccagcgaagt tttagcaaaa tggatttggg atcaggttaa acccgttgtg ccgctgttaa
    65521 gtgcggtgat ggtaaaagaa acctgcaccg caggttgtat ctatcgcggc gaatgataag
    65581 agtgtgtcgg cggtcaattt cccttaagta acgctatgtt agggtgttgt gttctggata
    65641 tctggggcat gacatggaag acgactgcga cattattatt atcggtgccg gtattgctgg
    65701 caccgcttgc gcgttacgct gcgcgcgagc gggtttatcc gttttgttac tggaacgagc
    65761 ggagattccc ggcagcaaaa atctttccgg cgggcggcta tacacccatg cgctcgcgga
    65821 actcctcccg caatttcacc tgaccgcgcc tcttgaacga cgcatcactc acgaaagtct
    65881 ttctctgtta acgccagatg gcgcaacgac gttttccagc ttactgcccg gcggtgaatc
    65941 ctggagtgta ttacgcgcac gattcgatcc gtggctggtt gccgaagccg aaaaagaagg
    66001 tgtcgaatgc atccccggtg cgacggtaga tgcactgtat gaagaaaacg gcagggtgtg
    66061 tggtgtcatt tgtggtgacg atattcttcg cgcccgttat gtggtgctgg cagaaggtgc
    66121 caacagcgtc ctggcggaac gccacgggtt agtgactcgt cctgctggcg aagcgatggc
    66181 attggggatc aaagaagtgc tgacgctgga accatccgct attgaagaac gttttcatct
    66241 ggagaataac gaaggcgcag cgttgttgtt cagcggcggg atctgtgatg acttacccgg
    66301 cggcgcattt ctttatacta atcaacaaac gctctcgtta gggattgttt gcccgctctc
    66361 ctcccttacg caaagtcgtg ttccggcaag cgagctgctg gctcgcttta aaacgcatcc
    66421 ggcagtgcgc ccgcttatca aaaacacgga atcactggag tatggtgcgc atctggtgcc
    66481 agaaggtggc ctgcgcagta tgccggtgca atacgccggt aacggctggc tgctggtggg
    66541 cgatgcgttg cgcagttgcg tcaataccgg aatttccgtg cgcggcatgg atatggcgct
    66601 aactggcgcg caggcggcgg cacaaacgct gataagcgcc tgccaacacc gcgagccgca
    66661 aaatctgttt ccgctttatc atcacaacgt agagcgcagc ctgctgtggg atgttctaca
    66721 gcgttatcag catgttccgg cgcttttgca acgtcctggc tggtatcggg tgtggcctgg
    66781 gttaatgcag gatatttccc gtgatttatg ggatcagggg aatacacctg ttccaccgct
    66841 gcgccagttg ctctggcgtc atttacgtcg tcacggcttg tggcatctga cgggcgatgt
    66901 tatcaggagt ctacgatgtc tgtagcccgt aatctctggc gcgctgccga tgcgccgcac
    66961 attgttccag ctgactccgt tgagcgccag acggcacaac ggttgattaa cgcctgtccg
    67021 gctggtcttt tttcgctcac acctgaaggt gacttacgcg ttgattatca cggctgcctg
    67081 gagtgtggca cctgccgttt gctgtgcgac gaatcaacac tacaacagtg gcgctatccg
    67141 ccttccggat tcggcatcac ctaccgcttt ggataaataa caaggataat ttatgtccct
    67201 cttacacctg ctccgccaga atccggtgat tgctgccgtt aaagataatg ccagcctgca
    67261 actggcaatc gactctgaat gccagtttat ttccgtgttg tacggcaata tctgcaccat
    67321 tagtaatatc gttaaaaaga ttaagaacgc cgggaaatat gcttttattc atgttgattt
    67381 gctggaaggc gcgtcaaata aagaagtggt gattcagttt ttgaaactgg tgaccgaggc
    67441 gtacggaatt atcagtacca aagcctcaat gctgaaagca gcaagagcgg aaggtttttt
    67501 ctgtattcat cgactgttta ttgttgattc gatttcgttt cacaacattg ataagcaagt
    67561 tacgcaatcg aatccggatt gtattgagat cctgccaggc tgcatgccta aagtgctggg
    67621 ctgggtgacg gagaaaatcc gccaaccgct gattgctggc gggctggtgt gcgatgaaga
    67681 agatgcgcgt aatgcgatta acgcgggtgt tgtggcgctt tccaccacga atactggggt
    67741 ctggacgtta gcgaaaaaat tgctttgacg ggataatcgt aacaagttga atttggtgtg
    67801 atttgtaggc tgcacgccac atccgacatt cagcgcctga tgcgacgctt gacgcgtctt
    67861 atcaggccta caagtcccca gcccagaacc atcggcagga tcatccggca gcacgttatt
    67921 ggcaatctgc gtgaatgttt gtcaccagtg cctccagcac cactttccag tcatcaacca
    67981 cgccaacatc tgcctgtgaa aataccgctg cactggcgtc atgattaatc gccaccacaa
    68041 acttgctatt gcgtacgccc gccattaacg ctgctgcgcc ggaagcgcca acaacaatac
    68101 acacctcagg tgccagcaga tgtccggaaa taccaatcac tttttcggca tcaacgccac
    68161 cgttcatcac tcgtgcccga ctgtagccca cttcggctcc catcttttct gccagcatag
    68221 cgatttcctg gttatctgcc tctccgccct gccccaccac cagcacccgt ctggcctcag
    68281 ctaatgggtc acgggtaaca tttttcaggt tttcgacgct gataagccag tctggaagcg
    68341 cgccggggac aatgttcagt tgctgcatcc caaagggcaa cgtggcgttt ttatctgctc
    68401 ccgcctgacg tgccagcgaa aggcatagcg ggcgtttttc tgtttgcagc gtcgccgtca
    68461 acgcatttcc ccagtgggat ttgcgcacgc ttacggttgg aatatctaac gaagtcacct
    68521 ggcagatgct tgcaccatgt aaacgccagg ccagccgggt cgacagttca tcgccaaacg
    68581 cgcccggagg aaaaaggaca acctccgcag gcgttcgttg ccactgttcc accagcgcat
    68641 ccagcacctg ttccgcaacc atcggctgtg gttctatttg ccaatgcgtt agtgtgctgc
    68701 cgctgaaatc ctgcgccgcc agccagcccg ccattgcggc atgttcctga tttattgtca
    68761 caattgcgat attcatggct gcatcctttg gcgtaaatag tcctgccata gcttttgcgc
    68821 tttttctgct accgtttgcc catcaatcag cgccgctctg cgtcgttgct ctggccttgc
    68881 cagttgcagg cactgtatcg ctggcgcttc cgcggcaaca gtttcgcggg taatctccgc
    68941 tttccccgtc gccattcgct ggcgcattcc cggtactggc aaggccactt caccacactg
    69001 acgcacggca atcaccgcag gcaagcgaac ccggcaacag cgcagtccat gttcagtacg
    69061 ctgttcgagg gtgataaacg gcgcgtcgag agtaaaacgc tccacctggg tgaagcaggg
    69121 ccaaaccagc atttccgcca acaaaaatgg cgtttgccca ttctgccctt cgctgctttg
    69181 gcagccagta attatcagat ccagcggatt ctggtactgc cattcggcaa tatggcgagc
    69241 gacaaattcc ggcgcaaagc gcagatctgc cgccgtctcc agcaatacag cttcctcaaa
    69301 cccaagtgcc atgagatagc gcagccagtg caacgcccgt tcatccccca tgctcaacgc
    69361 ggttaaagac atcggcgtac cgttttttct ctgcaccaac agcagcgcgg cagcggcctg
    69421 ttcatcagca ccgagtaaac ttcgcagtag cgaaacatcc ggtccgctat taccctgagc
    69481 cgccgccagc cactcttttt ccgccagcat cccggcatcc ggttcggctt taaacgctaa
    69541 cagtatgttc attcgcacct cctacatttc ccgccgccac cagcgggagt gctttggttt
    69601 ctggtgccca tagccaggtc acaacaaaac caaccaacaa cactgccgcc aggagcaata
    69661 aggtggcttg cattccccac tgtgccagca cccacggcag caggccagta cttaccgccg
    69721 cccccaaccg actcatggcg gtggcaaaac cgacgcccag cgagcgaatg tcagtaggaa
    69781 aactttccgc aggcaaaatg cccaccagat tactgactgc cgaaatggtg gtgctgaaga
    69841 gaacaaaaag cagcagcgtt aatgaactgc cggaaggcaa acaggccatc actaccagcg
    69901 ttgccgccag cagcaaaaaa cttcccagta aaaatctgcg atgcgctaac aagtgggtca
    69961 acactaaccc taataacgcg ccaacaatta acaacgcatt aagcatcagg ctggcagtca
    70021 gcgcatcttc cagaccaata gtttgggcga tggtcggcag ccaggtataa atcacaaacc
    70081 atgggattac gaggcagaca aagaagacgc tgttaaacgc cgtgcgccgc cagtagcgcg
    70141 aagagaacaa ggttttgatg tgtttatggg tcgccgttgc cacttcatcg cccagtaaaa
    70201 catgcgggcc aaaatagcga tgaacgatag cgtgagcttc tgcaaaacgc ccctggcgca
    70261 gtagccagcg tggcgattcc ggcgtgcccc aacgtagcag cgtaatcagc aaggctagta
    70321 acgccgctga tgccagcagc cagcgtggcg attccggcgt gccccaacgt agcagcgtaa
    70381 tcagcaaggc tggtaacgcc gctgatgcca gcagccagcg ccaggcctcc gggctttcgg
    70441 aaataaagtg atgtccggca atacttgcca gcacatagcc tacggtccac accacactga
    70501 atgcgcccag caaaataccg cgatggcggc gcggggaaaa ttcagccagc aaggtgtgac
    70561 cgactgagta atcgcctccc agcccaatgc cgatcaaaat gcgcaatcca ataagatgct
    70621 ctggcgtggt ggcaaaaaac tgtaaaaacg aagcaagcgt aatcagcata aaactgaagg
    70681 tgaagatttt ttgccgcccg atatggtcgg agatccaccc caaaaccagg ctgccaagaa
    70741 acaaaccaag taacgccgag ccgccgatca ttcccgccat aaacggcgtc agttgcatgg
    70801 cgggcgtaag ctgaataatg gcgtaaccaa taacgccgag gacataaccg tcggtcaggt
    70861 gtgcgccgaa agtgagcgca gcaattcgac agtgaaaacg gttaagcggt aaatcatcca
    70921 ttcgcaccgg tgaagtgttc atctttaccc cttcattgtc cggtaataag aacggcttct
    70981 cgcctgagaa gccgaaaagg caacgccccg tcatcgcgga acgctgcctg acacattatt
    71041 tttcaatcgg atagatagta cccgtgttca taatgccatt aggatcgaac tgctttttca
    71101 gcccttccag caacgcccac gcgctgccgt gttccagttt gctccagtga acgcgatgtt
    71161 taccgatacc gtggtggtgc accatcgaac cgccgagacg aatggtttct tcgcagatga
    71221 ttttgttgag cggattgtgg tacttgtcga tttcctcttc cggcttacag tcaacgacgt
    71281 tgtagtcgta gacgaagtac atgttggtgc cgttctggta gctgtgagag gagtgaccgc
    71341 ccagcatggt gatgtcatcg gcgtacggga actcggtacg aatacggtta ataacgcttt
    71401 cgtagatttc gtgaatgcag ctccagcagc cggacacttc agtggtaaag cccatgttgc
    71461 cggttttgag gatctgcaca cgttcggcag ctactttatc cggtccccag ttcaggttgt
    71521 taaaccaggt ttcgatcagc ttgctgtcca cgcgctggca ttgcgggtaa cgtgccacga
    71581 tttcagcaat cccttcgccc gttgccttcg ccatgcgagg attcccctca gccataaaga
    71641 tcagcacgca tttgccgtcg gcaaaatggg tgaagtgttg ggtgccatct tcagcatcat
    71701 acaaacgagc aatcgacgga cgataccctt ccaccatgat ttcacgcagg atgttgaagc
    71761 cggttttcat gtcttccagc acatagccat agaagagatt gttttccggc gtaaatttga
    71821 agattttcac tgtgacttca gtgatatagc acagtgcacc ttcgttgccg atgatgatat
    71881 gacgaatgtc cgggccagcc gcgcgacgtg gcacgttttt aatgcgtgtg acggtgccat
    71941 ctgccagcac cgcttccaga ccaacgacca tatcctcgat tgcgccgtag agtgtggaaa
    72001 actgcccgat actgcgggtc gccaccaggc cgcccatctg cgccagcggc tttgactgcg
    72061 gagaatgccc cgtggtgtaa cctttttcac gcaacgcgtt ttccagcacc tccagcggaa
    72121 cgccacattg cgccgtggcc tgcatattct caatatcaat attaatgatt tgattcatgg
    72181 cggagccgtc gagcaccacc gagttttcca caacggtttc cagcccacct tcggtagcgg
    72241 aagcaccggt acgcggcaca ccgttaattt tgtgcgcatt cataaaattc agcacacggg
    72301 atacttgttc tgtggaaccg agttttacga ccgctgccgg aatcggcaaa gtataaatgc
    72361 catgaatatc cggaaattta cgaaaacggt caatactgtt tttctttaat actgtttcat
    72421 cggtaattac gcgatctgca ccaacaattt cctttagctg gtcgaca
//
LOCUS       DAZFFE010000008        70903 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFFE010000008 DAZFFE010000000
VERSION     DAZFFE010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 70903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.008"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..457)
                     /locus_tag="ACHJ69_000679"
     CDS             complement(<1..457)
                     /locus_tag="ACHJ69_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO5013737.1"
                     /translation="MKVSTLGIDLAKNVFQLHGVDHEGHTILRKKLTRAKFVQFVIQL
                     EPCLIGMEACSSSHYFARLFTRYGHEVKLIPPQYVKPYVKTNKTDAADAEAICEAVTR
                     PNMRFVQIKTEEQQAVLALHTERGILIRERIACANSLRATLAEFGITIAA"
     gene            complement(689..1636)
                     /gene="dusC"
                     /locus_tag="ACHJ69_000680"
     CDS             complement(689..1636)
                     /gene="dusC"
                     /locus_tag="ACHJ69_000680"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO5013738.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            1875..2273
                     /gene="yohJ"
                     /locus_tag="ACHJ69_000681"
     CDS             1875..2273
                     /gene="yohJ"
                     /locus_tag="ACHJ69_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO5013739.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            2270..2965
                     /gene="yohK"
                     /locus_tag="ACHJ69_000682"
     CDS             2270..2965
                     /gene="yohK"
                     /locus_tag="ACHJ69_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO5013740.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            3095..3979
                     /gene="cdd"
                     /locus_tag="ACHJ69_000683"
     CDS             3095..3979
                     /gene="cdd"
                     /locus_tag="ACHJ69_000683"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO5013741.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKVDAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            4129..4848
                     /gene="sanA"
                     /locus_tag="ACHJ69_000684"
     CDS             4129..4848
                     /gene="sanA"
                     /locus_tag="ACHJ69_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO5013742.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            4851..5090
                     /gene="yeiS"
                     /locus_tag="ACHJ69_000685"
     CDS             4851..5090
                     /gene="yeiS"
                     /locus_tag="ACHJ69_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO5013743.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            5284..6522
                     /gene="preT"
                     /locus_tag="ACHJ69_000686"
     CDS             5284..6522
                     /gene="preT"
                     /locus_tag="ACHJ69_000686"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO5013744.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYLPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIETQHYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAGAIHHYLEGACSC"
     gene            6516..7751
                     /gene="preA"
                     /locus_tag="ACHJ69_000687"
     CDS             6516..7751
                     /gene="preA"
                     /locus_tag="ACHJ69_000687"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO5013745.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKALKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANHNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(7994..9004)
                     /gene="mglC"
                     /locus_tag="ACHJ69_000688"
     CDS             complement(7994..9004)
                     /gene="mglC"
                     /locus_tag="ACHJ69_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO5013746.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCVIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVVFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(9020..10540)
                     /gene="mglA"
                     /locus_tag="ACHJ69_000689"
     CDS             complement(9020..10540)
                     /gene="mglA"
                     /locus_tag="ACHJ69_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO5013747.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQSEILRLASLHL"
     gene            complement(10601..11599)
                     /gene="mglB"
                     /locus_tag="ACHJ69_000690"
     CDS             complement(10601..11599)
                     /gene="mglB"
                     /locus_tag="ACHJ69_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO5013748.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(11879..12919)
                     /gene="galS"
                     /locus_tag="ACHJ69_000691"
     CDS             complement(11879..12919)
                     /gene="galS"
                     /locus_tag="ACHJ69_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO5013749.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAADNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(13061..14218)
                     /gene="yeiB"
                     /locus_tag="ACHJ69_000692"
     CDS             complement(13061..14218)
                     /gene="yeiB"
                     /locus_tag="ACHJ69_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO5013750.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGGISDSQTSRA
                     WTPDTSAILYEKHWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWRLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANIFFSVIWLRYFRQGPVEWLWRQLTLRAAGPTISKTSR"
     gene            complement(14235..14903)
                     /gene="folE"
                     /locus_tag="ACHJ69_000693"
     CDS             complement(14235..14903)
                     /gene="folE"
                     /locus_tag="ACHJ69_000693"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO5013751.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            15161..15997
                     /gene="yeiG"
                     /locus_tag="ACHJ69_000694"
     CDS             15161..15997
                     /gene="yeiG"
                     /locus_tag="ACHJ69_000694"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO5013752.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWRIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(16029..18020)
                     /gene="cirA"
                     /locus_tag="ACHJ69_000695"
                     /pseudo
     CDS             complement(16029..18020)
                     /gene="cirA"
                     /locus_tag="ACHJ69_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(18312..19781)
                     /gene="lysP"
                     /locus_tag="ACHJ69_000696"
     CDS             complement(18312..19781)
                     /gene="lysP"
                     /locus_tag="ACHJ69_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO5013753.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(19986..20867)
                     /gene="yieE"
                     /locus_tag="ACHJ69_000697"
     CDS             complement(19986..20867)
                     /gene="yieE"
                     /locus_tag="ACHJ69_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO5013754.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRICASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20966..22015
                     /gene="yeiH"
                     /locus_tag="ACHJ69_000698"
     CDS             20966..22015
                     /gene="yeiH"
                     /locus_tag="ACHJ69_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO5013755.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            22089..22946
                     /gene="nfo"
                     /locus_tag="ACHJ69_000699"
     CDS             22089..22946
                     /gene="nfo"
                     /locus_tag="ACHJ69_000699"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO5013756.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIVLDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            22950..24038
                     /locus_tag="ACHJ69_000700"
     CDS             22950..24038
                     /locus_tag="ACHJ69_000700"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO5013757.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNITPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAIA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(24306..25247)
                     /gene="rihB"
                     /locus_tag="ACHJ69_000701"
     CDS             complement(24306..25247)
                     /gene="rihB"
                     /locus_tag="ACHJ69_000701"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO5013758.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAENT
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMIGLDLTNQTVCTPDVIARIERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            25377..26075
                     /gene="yeiL"
                     /locus_tag="ACHJ69_000702"
     CDS             25377..26075
                     /gene="yeiL"
                     /locus_tag="ACHJ69_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO5013759.1"
                     /translation="MKEIHNNDLKQQLISESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYVTLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFINDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(26142..27392)
                     /gene="psuT"
                     /locus_tag="ACHJ69_000703"
     CDS             complement(26142..27392)
                     /gene="psuT"
                     /locus_tag="ACHJ69_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO5013760.1"
                     /translation="MDIMRSVVGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGALDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(27486..28424)
                     /gene="psuG"
                     /locus_tag="ACHJ69_000704"
     CDS             complement(27486..28424)
                     /gene="psuG"
                     /locus_tag="ACHJ69_000704"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO5013761.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQSAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(28412..29353)
                     /gene="psuK"
                     /locus_tag="ACHJ69_000705"
     CDS             complement(28412..29353)
                     /gene="psuK"
                     /locus_tag="ACHJ69_000705"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO5013762.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISVEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVTSLVENTECLN"
     gene            complement(29809..31500)
                     /gene="fruA"
                     /locus_tag="ACHJ69_000706"
     CDS             complement(29809..31500)
                     /gene="fruA"
                     /locus_tag="ACHJ69_000706"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO5013763.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNEAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKTQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(31517..32455)
                     /gene="fruK"
                     /locus_tag="ACHJ69_000707"
     CDS             complement(31517..32455)
                     /gene="fruK"
                     /locus_tag="ACHJ69_000707"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO5013764.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(32455..33585)
                     /gene="fruB"
                     /locus_tag="ACHJ69_000708"
     CDS             complement(32455..33585)
                     /gene="fruB"
                     /locus_tag="ACHJ69_000708"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO5013765.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            33952..35133
                     /gene="setB"
                     /locus_tag="ACHJ69_000709"
     CDS             33952..35133
                     /gene="setB"
                     /locus_tag="ACHJ69_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO5013766.1"
                     /translation="MHNSPAVTSAKSFDLTSMAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(35130..35384)
                     /gene="yeiW"
                     /locus_tag="ACHJ69_000710"
     CDS             complement(35130..35384)
                     /gene="yeiW"
                     /locus_tag="ACHJ69_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO5013767.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            35539..36111
                     /gene="yeiP"
                     /locus_tag="ACHJ69_000711"
     CDS             35539..36111
                     /gene="yeiP"
                     /locus_tag="ACHJ69_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO5013768.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            36334..37800
                     /gene="yeiQ"
                     /locus_tag="ACHJ69_000712"
                     /pseudo
     CDS             36334..37800
                     /gene="yeiQ"
                     /locus_tag="ACHJ69_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            37918..38904
                     /gene="yeiR"
                     /locus_tag="ACHJ69_000713"
     CDS             37918..38904
                     /gene="yeiR"
                     /locus_tag="ACHJ69_000713"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO5013769.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALERWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHQYSVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFNTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            38943..39656
                     /gene="lpxT"
                     /locus_tag="ACHJ69_000714"
     CDS             38943..39656
                     /gene="lpxT"
                     /locus_tag="ACHJ69_000714"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO5013770.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPINHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            40068..40634
                     /gene="mepS"
                     /locus_tag="ACHJ69_000715"
     CDS             40068..40634
                     /gene="mepS"
                     /locus_tag="ACHJ69_000715"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO5013771.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            40815..42365
                     /gene="pdeN"
                     /locus_tag="ACHJ69_000716"
     CDS             40815..42365
                     /gene="pdeN"
                     /locus_tag="ACHJ69_000716"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO5013772.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGLALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTFVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVVYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAEAQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPAHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEREATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTP"
     gene            42453..44267
                     /gene="yejA"
                     /locus_tag="ACHJ69_000717"
     CDS             42453..44267
                     /gene="yejA"
                     /locus_tag="ACHJ69_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO5013773.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGLPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYSKDHKLSDPLATPPLASGPYRITYW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNVTTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKATKLPSARQQGE"
     gene            44268..45362
                     /gene="yejB"
                     /locus_tag="ACHJ69_000718"
     CDS             44268..45362
                     /gene="yejB"
                     /locus_tag="ACHJ69_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO5013774.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            45362..46387
                     /gene="yejE"
                     /locus_tag="ACHJ69_000719"
     CDS             45362..46387
                     /gene="yejE"
                     /locus_tag="ACHJ69_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5013775.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLLLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            46389..47978
                     /gene="yejF"
                     /locus_tag="ACHJ69_000720"
     CDS             46389..47978
                     /gene="yejF"
                     /locus_tag="ACHJ69_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO5013776.1"
                     /translation="MTQTLLSIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHHVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSHGSIVFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAASQQEYTRQLLALS"
     gene            complement(47982..48326)
                     /gene="yejG"
                     /locus_tag="ACHJ69_000721"
     CDS             complement(47982..48326)
                     /gene="yejG"
                     /locus_tag="ACHJ69_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO5013777.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(48660..49850)
                     /gene="bcr"
                     /locus_tag="ACHJ69_000722"
     CDS             complement(48660..49850)
                     /gene="bcr"
                     /locus_tag="ACHJ69_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO5013778.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNILFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(49878..50573)
                     /gene="rsuA"
                     /locus_tag="ACHJ69_000723"
     CDS             complement(49878..50573)
                     /gene="rsuA"
                     /locus_tag="ACHJ69_000723"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO5013779.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVI"
     gene            50723..52483
                     /gene="radD"
                     /locus_tag="ACHJ69_000724"
     CDS             50723..52483
                     /gene="radD"
                     /locus_tag="ACHJ69_000724"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO5013780.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVIFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGSERDVLIDDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            52608..52892
                     /gene="rplY"
                     /locus_tag="ACHJ69_000725"
     CDS             52608..52892
                     /gene="rplY"
                     /locus_tag="ACHJ69_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO5013781.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(53031..54038)
                     /gene="yejK"
                     /locus_tag="ACHJ69_000726"
     CDS             complement(53031..54038)
                     /gene="yejK"
                     /locus_tag="ACHJ69_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO5013782.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            54220..54447
                     /gene="yejL"
                     /locus_tag="ACHJ69_000727"
     CDS             54220..54447
                     /gene="yejL"
                     /locus_tag="ACHJ69_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO5013783.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            54467..56227
                     /gene="yejM"
                     /locus_tag="ACHJ69_000728"
     CDS             54467..56227
                     /gene="yejM"
                     /locus_tag="ACHJ69_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO5013784.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            56302..56378
                     /locus_tag="ACHJ69_000729"
     tRNA            56302..56378
                     /locus_tag="ACHJ69_000729"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56336..56338,aa:Pro,seq:ggg)
     gene            complement(56510..57432)
                     /locus_tag="ACHJ69_000730"
                     /pseudo
     CDS             complement(56510..57432)
                     /locus_tag="ACHJ69_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            57752..58399
                     /gene="narP"
                     /locus_tag="ACHJ69_000731"
     CDS             57752..58399
                     /gene="narP"
                     /locus_tag="ACHJ69_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO5013785.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDSGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(58434..59486)
                     /gene="ccmH"
                     /locus_tag="ACHJ69_000732"
     CDS             complement(58434..59486)
                     /gene="ccmH"
                     /locus_tag="ACHJ69_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO5013786.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(59483..60040)
                     /gene="dsbE"
                     /locus_tag="ACHJ69_000733"
     CDS             complement(59483..60040)
                     /gene="dsbE"
                     /locus_tag="ACHJ69_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO5013787.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(60037..61980)
                     /gene="ccmF"
                     /locus_tag="ACHJ69_000734"
     CDS             complement(60037..61980)
                     /gene="ccmF"
                     /locus_tag="ACHJ69_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO5013788.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVNPQKTAPEAA"
     gene            complement(61977..62456)
                     /gene="ccmE"
                     /locus_tag="ACHJ69_000735"
     CDS             complement(61977..62456)
                     /gene="ccmE"
                     /locus_tag="ACHJ69_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO5013789.1"
                     /translation="MNIRRKNRLWIACSVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(62453..62662)
                     /gene="ccmD"
                     /locus_tag="ACHJ69_000736"
     CDS             complement(62453..62662)
                     /gene="ccmD"
                     /locus_tag="ACHJ69_000736"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO5013790.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(62659..63396)
                     /gene="ccmC"
                     /locus_tag="ACHJ69_000737"
     CDS             complement(62659..63396)
                     /gene="ccmC"
                     /locus_tag="ACHJ69_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO5013791.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(63438..64100)
                     /gene="ccmB"
                     /locus_tag="ACHJ69_000738"
     CDS             complement(63438..64100)
                     /gene="ccmB"
                     /locus_tag="ACHJ69_000738"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO5013792.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAAVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(64097..64720)
                     /gene="ccmA"
                     /locus_tag="ACHJ69_000739"
     CDS             complement(64097..64720)
                     /gene="ccmA"
                     /locus_tag="ACHJ69_000739"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO5013793.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTGAA"
     gene            complement(64733..65335)
                     /gene="napC"
                     /locus_tag="ACHJ69_000740"
     CDS             complement(64733..65335)
                     /gene="napC"
                     /locus_tag="ACHJ69_000740"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO5013794.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(65345..65794)
                     /gene="napB"
                     /locus_tag="ACHJ69_000741"
     CDS             complement(65345..65794)
                     /gene="napB"
                     /locus_tag="ACHJ69_000741"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO5013795.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(65791..66654)
                     /gene="napH"
                     /locus_tag="ACHJ69_000742"
     CDS             complement(65791..66654)
                     /gene="napH"
                     /locus_tag="ACHJ69_000742"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO5013796.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(66641..67336)
                     /gene="napG"
                     /locus_tag="ACHJ69_000743"
     CDS             complement(66641..67336)
                     /gene="napG"
                     /locus_tag="ACHJ69_000743"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO5013797.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLATVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDVA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(67343..69829)
                     /gene="napA"
                     /locus_tag="ACHJ69_000744"
     CDS             complement(67343..69829)
                     /gene="napA"
                     /locus_tag="ACHJ69_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO5013798.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEDKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGSNMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(69826..70089)
                     /gene="napD"
                     /locus_tag="ACHJ69_000745"
     CDS             complement(69826..70089)
                     /gene="napD"
                     /locus_tag="ACHJ69_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO5013799.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFTGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(70079..70573)
                     /gene="napF"
                     /locus_tag="ACHJ69_000746"
     CDS             complement(70079..70573)
                     /gene="napF"
                     /locus_tag="ACHJ69_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO5013800.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAANCPVSAITAEY
                     LHAH"
     gene            70682..70846
                     /locus_tag="ACHJ69_000747"
     CDS             70682..70846
                     /locus_tag="ACHJ69_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013801.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCFLIRI"
BASE COUNT        17420 a        18859 c        17403 g        17221 t
ORIGIN      
        1 cggccgcaat cgtaatacca aactcagcaa gtgtggctct taaactattg gcacaggcaa
       61 tccgctcacg gataagtatt ccccgttcag tgtgtaacgc taaaacggcc tgctgctctt
      121 cggtttttat ctgaacaaaa cgcatattcg gacgggttac cgcttcgcag attgcttcag
      181 catctgctgc atccgtcttg ttcgttttca cataaggctt cacatactgc ggaggtatga
      241 gttttacctc atgaccatag cgggtgaata atcgcgcaaa ataatgactg gatgagcagg
      301 cttccatgcc aatcagacaa ggttccagtt gaatcacaaa ctgaacaaac ttagcccggg
      361 tgagtttttt acgcaaaata gtatggcctt catgatcgac accatgaagc tgaaaaacgt
      421 tctttgccag gtcgatacca agagttgata ctttcatgtg gacgctccta atttttcagg
      481 acgtattgca acgatccagt ctggtacttt gataccgtaa aatctggagc gtccatctca
      541 ttatccctgt aaaacgcgcc ctcagcccac atccatgtgg gctgccccag ccttcaggga
      601 acgcctcggc gattttgacg ccaccaaaca accgtgcggc ctattgataa agagctaaca
      661 cattgtcaaa aaacatcact atggtttctt agagtttctc gatatcaatg gcctgaatag
      721 cccttgcaat atcaggggaa ttattcaaca cccgaacatg ctgaaataat tccgttgctt
      781 catcgtattc tttacgcaaa taactcaacc actgtttaat ccgcgcaacg tgatataacc
      841 cggtatcgcc ctgcttttcc agacgggtat atttttgcag caaagcaacc acctccggcc
      901 acggcattcg cggttcgtta tatttcacca cacggctcag gttgggaata ttgagcgccc
      961 cgcgaccaat catcactgcg tcgcagccgc tgatcgccat gcattgttgc gcactctgcc
     1021 agtcccagat ttcaccgttg gcaatcaccg gaatattaag ccgctggcga atctcgccaa
     1081 tcgcctgcca gtcaatatgt tccgcgcggt aaccctgctc ttttgtccgc ccatgtacta
     1141 ccagctccgt agcgccagcc tgttgcaccg catcggcgat ttcaaatttc ttctcaccgc
     1201 tgtcccagcc cagacgcact tttacgctga caggcaaatg cgccggtaca gcttcgcgca
     1261 tcgcttttgc accctggtag atgagttcag gatctttcag taacgttgcc ccaccaccgc
     1321 taccgttaac cgttttcgac gggcagccgc agttaagatc cacgccccag gaacctaact
     1381 ccaccgcacg ggcggcgttc tctgccagcc actgtgggaa ctgccctaat aactgcacgc
     1441 gaaccagcgt accggatggt gtccggctgg cattttgtag ctcagggcaa atgcgatgaa
     1501 agacttttac tggcagcagt tgatccacca cgcggacaaa ctcggtgatg cacagatcgt
     1561 agtcgttaac ttcggtcagt aattcccgca ccaaagagtc aagtacaccc tccatcggag
     1621 ccagtaacac acgcatatca tcacccgcaa aaaaatgagg cgctatgtta gcgcctccgg
     1681 tcaacggatt aaaggtctgc aaagagggat tgctgcaaac tgcaatccct ctctatgctt
     1741 gatgtatgcc tgagttcgct gcgcattgcg taataccggg gagcgaaaaa ataagcgctg
     1801 atttcttaat gtgatcggta gcacgtttta cgaattaatt gtatgataaa tccatctcat
     1861 ctggggtgtt gattatgagt aagacactga acattatctg gcaatattta cgcgctttcg
     1921 tcctgattta tgcctgcctg tatgcaggca ttttcattgc ttccctgcta ccggtaacca
     1981 ttccgggcag catcatcggg atgctgatcc tgtttgtcct gctggccttg caaattcttc
     2041 cggcgaaatg ggtcaatccg gggtgctacg tactgattcg ctatatggcg ctattgtttg
     2101 tgccgattgg cgtaggcgtc atgcaatatt ttgatttgct ccgcgcacag tttggtccgg
     2161 tagtggtttc ctgtgcagtc agtacgctgg tggtttttct ggtggtgagc tggagttcac
     2221 aactggtgca cggtgaacgt aaagtcgtag gtcagaaagg atcagaagaa tgatggcgaa
     2281 tatctggtgg tcattaccgc tgacgttgat tgtctttttt gccgcccgca aactggcggc
     2341 acggtataag tttcccctgc ttaacccgtt gctggtagca atggtggtga tcattccttt
     2401 tttaatgctg actggcatct cttacgacag ctactttaaa ggtagcgaag tgctaaacga
     2461 tctgctgcaa ccggcggtcg tcgcactggc ttatccttta tatgagcagc tacaccagat
     2521 ccgcgcgcgc tggaaatcga tcatcaccat ctgttttatc ggcagcgtgg ttgcgatggt
     2581 gacggggact tccgtggcat tgttgatggg cgcttcaccg gaaatcgccg cgtcaatcct
     2641 gccgaaatca gttaccacgc ctattgcaat ggcggttggc ggcagtattg gcggtattcc
     2701 ggcaatcagc gcagtttgcg tgattttcgt cggcatcctc ggcgctgtgt ttggccatac
     2761 attgcttaat gcgatgcgta ttcgtaccaa agctgcgcgc ggtctggcaa tgggcacagc
     2821 ctcgcacgcc ctcggtacgg cgcgctgcgc cgagctggat tatcaggaag gtgcatttag
     2881 ttcgctggcg ctggtgttat gcgggattat tacttcgctg atcgcaccgt tccttttccc
     2941 gattattctg gcagtaatgg gctaaaattt gcgatgcgtc gcgcattttt gatgtaagtt
     3001 tcacgcgttg cataattaat gagattcaga tcacatataa agccataacg ggttcgtaaa
     3061 ctgttatccc attacatgat tatgaggcaa cgccatgcat ccacgttttc aaaccgcttt
     3121 tgcccaactt gcggataact tgcaatctgc actggaacct attctggcag acaagtactt
     3181 ccccgctttg ttgaccgggg agcaagtctc atcgctgaag agcgcaacgg ggctggacga
     3241 agacgcgctg gcattcgcac tacttccgct ggcggcagcc tgtgcgcgta cgccattgtc
     3301 gaattttaat gttggcgcaa ttgcgcgcgg tgtgagcgga acctggtatt tcggtgccaa
     3361 tatggaattt attggtgcga caatgcagca aaccgttcat gccgaacaaa gcgcgatcag
     3421 ccacgcctgg ctgagtggtg aaaaagcgct tgcagccatc accgttaact acacgccttg
     3481 tggtcactgc cgtcagttta tgaatgaact gaacagcggt ctggatctgc gtattcatct
     3541 tccgggacgc gaggcacacg cgctgcgtga ctatctaccg gatgcctttg ggccgaaaga
     3601 tctggagatt aaaacgctgc tgatggacga gcaggatcac ggctatgcgc tgactggtga
     3661 tgcgctttct caggcggcga ttgcggcggc aaaccgttcg cacatgcctt acagtaagtc
     3721 gccaagcggt gtcgcgctgg aatgtaaaga cggtcgtatt ttcagcggca gctacgctga
     3781 aaacgccgca ttcaacccga ctctgccgcc gttgcagggg gcgttaattc tgttgaatct
     3841 caagggttat gattacccgg atatccagcg cgcggttctg gcagaaaaag tcgatgcgcc
     3901 gttgattcag tgggatgcca cctccgcaac gctgaaagct ctcggctgtc acaatatcga
     3961 ccgcgtgctt ctcgcttaag cctggtgccg gatgcggcgt gaacgccttg tccggcttgc
     4021 cagccctttc ctggtgtcga aaatcccggc aaacagtttg ccgtttcttg cgcaaaacca
     4081 gcgggtaaag tagcctgatg gaaattttcc ttagatcgag tctcctgcat gttaaagcgc
     4141 gtgttcctca gcctgttagt cctgatcggc ttgctgctgt tgactgtgct cggcctcgat
     4201 cgctggatga gctggaaaac cgcgccttat atctacgacg aattgcagga tctcccctac
     4261 cgccaggtcg gtgtggtgct cggcacagca aaatattatc ggactggcgt aattaatcag
     4321 tattatcgct accgcattca aggagcgatt aatgcctata acagcggtaa ggtaaattat
     4381 ctattactga gcggcgataa cgcattgcaa agttataatg agccgatgac catgcgcaaa
     4441 gatttaatcg cagctggtgt cgacccatca gatattgttc tcgattacgc aggctttcgt
     4501 acgctggatt ccatcgtgcg tacacgcaaa gttttcgata ctaatgattt cattattatc
     4561 acccaacgtt tccactgtga gcgggcatta tttattgcgc tgcatatggg gattcaggct
     4621 cagtgttatg ccgtaccgtc accgaaagat atgctgtcag tacgtattcg cgaatttgcc
     4681 gcccgtttcg gtgcgctggc tgacctttat atttttaaac gtgaaccgcg ttttttaggg
     4741 ccgctggtcc ctattccggc tatgcaccag gtgccggaag atgcgcaggg gtatcccgcc
     4801 gtcacacccg aacagttact tgaattacaa aagaaacaag gaaagtagtt atggatgtac
     4861 agcagttttt tgtcgttgcc gtttttttcc ttatcccgat attttgtttc cgcgaagcat
     4921 ggaaaggctg gcgcgcaggc gcgattgata aacgggttaa aaatgcacca gaaccggtgt
     4981 atgtctggcg agcgaaaaat cccggactct tcttcgctta tatggtggca tatatcggct
     5041 tcggaatttt atctatcggc atgattgttt atcttatttt ctatcgttaa tattttccct
     5101 tttctgttaa ttattgagaa taattattac ttcactcaat aagctgcgga tataacattc
     5161 tgaaccgcag ctatttgtga atcctttcac agtttaaata tccccacgcg catagcctta
     5221 atatcagcac gcaatattta tttagcgttc gccttaatta ctcatagcat taaggaagat
     5281 cacatgccgc aacaaaatta tctggatgaa ctcactccgg cttttacgcc tttactggcg
     5341 attaaagaag cctctcgctg tttattatgt catgacgctc cctgtagtca ggcttgcccg
     5401 gcacagaccg atccggggaa atttattcgc tcaatctact ttcgtaattt taaaggcgct
     5461 gccgagacaa ttcgcgaaaa taatgccctc ggtgccgttt gtgccagagt gtgcccgacg
     5521 gagaaattat gccaaagcgg ttgtacacgt gccggtgttg atgctcccat tgatatcggt
     5581 cgcttacagc gttttgttac tgattttgaa caacaaactg ggatggaaat ttatctgccc
     5641 ggtactaaaa cgctcggcaa agtcgccatt attggcgcag gtcctgccgg attgcaggcc
     5701 agtgtgacgc tgacaaacca gggttatgac gtcacgatct atgagaaaga agcacaaccc
     5761 ggtggctggt tgcgtaacgg cattccgcaa ttccggttac cgcaatcggt gctggatgca
     5821 gaaatcgccc gtatagaaaa aatgggcgtg accattaagt gcaataacga aatcggtaaa
     5881 acactcaccc tggagcagct gaaagcggaa aaccgcgcgg tactggtcac cgtagggtta
     5941 tcgagcggtt ccgggttatc gctgtttgag catagcgacg ttgagattgc cgtcgacttc
     6001 ctgcaacgtg cacgacaggc gcaaggcgat atcagcattc cacaaagcgc attaattatc
     6061 ggcggcggtg atgttgcgat ggacgtagcc agcacgctga aagttctcgg ctgtcaggcg
     6121 gtaacctgcg tagcgcgtga agagttagat gagtttccgg caagcgaaaa agagtttacc
     6181 agcgcccggg agctgggcgt ttcgatcatc gatggattta caccagtagc cgtggaaggc
     6241 aataaagtca cctttaagca tgttcgttta ccgggcgaac tgacgatggc ggcagataaa
     6301 attattctcg ccgtcggtca gcatgccaga ctggatgcct ttgcggagtt agagccgcag
     6361 cgtaacacca tcgaaacgca acattaccag acccgcgacc cgcaagtctt tgctgctggc
     6421 gatattgttg agggtgacaa aaccgtggtc tatgcagtga aaaccgggaa agaagccgcc
     6481 ggggcgattc atcactattt agagggagct tgctcatgtt aacgaaagat ctttcgatta
     6541 ctttttgcgg cgtgaagttt cccaacccgt tctgcctttc ttcttcgccg gtaggcaact
     6601 gctatgagat gtgtgccaaa gcctacgaca caggttgggg cggcgtggtg tttaaaacga
     6661 tcggtttttt tatcgccaac gaagtctcgc cgcgttttga tcatctggtg aaagaagata
     6721 ccggttttat cggcttcaaa aatatggagc agattgctga acatccgttg gaagagaatc
     6781 tggccgccct gcgtcggctg aaagaagatt atccggacaa agtattgatc gcttcaatca
     6841 tgggcgaaaa tgagcagcaa tgggaagagc tggcgcgtct ggtgcaagaa gctggcgcgg
     6901 atatgatcga gtgtaacttc tcctgtccgc aaatgacttc tcatgcgatg ggtagcgatg
     6961 tcgggcaaag cccggagctg gtagaaaaat attgtcgggc agtaaaacgt ggttccacgc
     7021 tgccaatgct ggcgaagatg acgccgaata ttggtgatat gtgcgaagtg gcgctggcgg
     7081 cgaagcgcgg cggcgcagat ggcattgcgg cgattaacac cgttaaatcc atcaccaata
     7141 tcgatcttaa tcagaaaatt ggtatgccga tcgttaacgg taaatcgagt atttccggat
     7201 attccggtaa agcgctaaaa ccgatcgccc tacgctttat tcagcaaatg cgcacccatc
     7261 cagaactgcg cgatttcccg atcagcggta tcggcggcat tgaaacctgg gaagatgcgg
     7321 cggagttttt attgctcggt gcagcgacgt tgcaggtgac caccggcatt atgcagtacg
     7381 ggtatcggat agtggaagat atggcaagcg ggttgtcgca ttatctcgcc gatcaagggt
     7441 ttgattcgtt gcaggagatg gtaggtctgg cgaatcacaa tattgtcccg gcggaagatt
     7501 tagaccgcag ttatattgtc tatccccata tcaatcttga taaatgtgtt ggctgtggac
     7561 gctgttatat ttcctgttac gacggcggtc accaggcgat ggaatggagc gagaaaaccc
     7621 gcacaccgca ttgtaatacc gagaaatgcg tgggttgtct gctttgtggt catgtctgcc
     7681 cggtaggttg tattgatctt ggggaagtga agtttaagaa aggcgagaaa gaacacccgg
     7741 taacgttgta aaaccagcga cgcatccggc atttagttcc tgtcgccgga tgcggcgtga
     7801 acgccttatc cggcctacaa aaccataaaa atcagtatgt tgagcttatt ataggccgga
     7861 taaaacgcgc cagcgttgca tccggcattt ggctcctgtc gccggatgcg gcgtgaacgc
     7921 cttatccgga ctacaaaacc acacagatca tgatgttatg tgcgtttatg aacgaatgcg
     7981 gaccacaatc acatcacttc ttacgcgcgt atttcagtga atccagcgcc acggcgaaga
     8041 taataatcgc ccctttgatg atgtactgcc agtacgggtt tacgccgata tacgtcaggc
     8101 catagttaat gacggtaaaa ataattaccc cggtcaccac accaattacc gtccccacac
     8161 cgccgctgaa cgatacgccg cctaccacgc acgccgcgat agcatccagc tcatacataa
     8221 agccgaggtt gttggtggct gagccgatac gtccggcttc taacatcccg ccgaaggcat
     8281 agaacacacc agacaacgcg taaatcatca gcaggttcag gccgacgttg acaccagata
     8341 ctttcgccgc ttccgggtta ccgccaatgg caaaaatgtt cttgccgaag cgggttttgt
     8401 tccataacac ccagacaaac accaccgcaa tcaacgcgta gaaggtgatg taagagagac
     8461 ggaaactccc cagcgcgaca aagccttgag caaaggtaga gaagccactg tcaaaaccgg
     8521 aaattggcga cgccccgaca aagtcgtaat agagcgagtt aatgccgtag acgataatca
     8581 tcgtaccgag cgtggtaatg aacggcgtca cgttgagata agcgataatc aagccgttga
     8641 tcaaaccgat caccgcacca atgacacaga caatcagaat aaccagcgca atcggcatcg
     8701 tcgccatttc cgggaacact ttgttggcgt tatccatgga ctgcaataac gtcgctgcta
     8761 ctactgccgc cagccccacc tggcgacctg cggaaaggtc agtcccctgg gtgacaatta
     8821 accctgccac accaagcgcg ataataatac gcaccgatga ctgggtgaga atattactta
     8881 agttcaacag acttaaaaat gttgggtcct ggaaaataat aatcgccagc aaaactaaaa
     8941 gaacgacgta aataccgccc tctttcaggt aagtaagaaa acttttctta tttaacgcac
     9001 tcatgggaag cccctaatct taaaggtgca aagacgcaag acgcagaatt tcgctttgcg
     9061 ttgttgtttt tgtatcgaca attccggaaa cgagaccatt gctcatgacc agaatacggt
     9121 ctgttatccc taacaactct ggcatttcgg aggagataat aataatcccc ttgcctttct
     9181 tcgccagttc ggcaattaac tgataaatct caaacttagc cccaacatca ataccgcgcg
     9241 tcggttcatc gagcattaat atttctggtt gcgttaacag ccagcgacca ataatcactt
     9301 tttgctgatt accaccggag agcgaaccaa tttgcgtccg atgccccggt gtttttaccc
     9361 gcatcgaatc aatcacccac tgggtatcgc ttttcatccg cgagttatcc agtaaaccaa
     9421 ctttattttt atagttgcgg atattggaaa ttaacgagtt aaagccaata tccagatagg
     9481 cataaattcc cgttgagcgg cgctcttcag ttaccagtgc aaatccgtgg tttatggctt
     9541 cgttggcatt atggttattg atctttttgc cgtgcaacgt aatggtgcca gccgatttct
     9601 cgcgaatacc aaataacgtc tcaacaatat cggtacgttt cgctcccacc agaccggcta
     9661 taccgagaat ctccccttta tgcagatcaa acgagacatc gcgtatcgac ggctggcgca
     9721 gcgacgtcag gttacgcact tccagaatca cttcgcccgg cttgttttct ttgtcaggga
     9781 aacgctggtt aagagaacgc ccaaccatca tggcgatgat cttgtccatc gtcagtcctg
     9841 ccagcggttc ggtagcgatc cactgaccgt cgcgcaatac ggtaacttca tcacataact
     9901 ggaagatttc ttccatttta tgcgagatat aaacaatacc gcagccgcgc tcttttaatt
     9961 tacgaataat agtgaacaga tgattgacct ctttttcggt taacgaagaa gtcggttcat
    10021 ccataatcac aattttcgcg ttataggaaa acgctttggc aatttcgatc atctgcattt
    10081 gcgaaacaga taatgtaccg acgcgcgcac gcggatcgat atcaatatcc agttcatcaa
    10141 aaatcgcttt ggtttcgcgg tacattttgt cctgatcgac aaacatgcct ttggtgggat
    10201 atcgccccag ccacatgttg tccatcaccg aacgttgtaa taccaggttt aactcctggt
    10261 gtaccatcga aataccattt tccagtgctt ctttggcgga atggaaatcg atctctttac
    10321 cctggaataa aatggtgccg gagtcttttt gataaatacc aaacaggcat tttaataatg
    10381 tcgattttcc tgcaccgttt tcccccatta atgcatggat agaatgtggt cggactttta
    10441 aattaacgtt atcaagtgcc ttaacaccag gaaaagactt gttgataccg ctcatttcca
    10501 acaagtattc cccggaagac ggagtcgttg agctgaccat ataattttac cttgttggcc
    10561 atacaataag ggcgcagtaa acgactgcgc ccaatcagtc ttatttcttg ctgaattcag
    10621 ccaggttatc tttatctacg ccaacataag gtacgcggac cactttgttg tcgattttcc
    10681 agttggtgcc atcagccgca cctttgccat cggccaggtt tttcgccaga tcaaaggtcg
    10741 ctttcgcctg gttgttagca tcgttcagta cggtgcccgc cagtgcaccg gatttcacca
    10801 gcgccagcgc ttctggcagc gcatcgacgc caaacaccgg aatgctggac ttgttgtgtg
    10861 ctttcagtgc ttctaccgcg cccattgcca tcgcgtcgtt gttggcgata accacctcga
    10921 ttttgttggc gttcgggcca gacagccagg cgtccatctt atctttcgcc tgagcggtat
    10981 cccacatagc ggtatctaac tgtaactgtt cagttttaat gcctttgtcg ttcaattctt
    11041 taatcacgta agtggtacgt gcttctgcat ccggatgccc cggctcacct ttcagcagta
    11101 cgaattgaat ctgaccgtct ttgttcagat cccaaccctg attcgccgcc cagtgtttag
    11161 caatcaaatc gccctgaata atgccggact ctttggagtc agtgccaacg tagtaggctt
    11221 tgtcgtagct atccagcgcc ttacgagacg gttctttgtt gaagaaaacc accggcacgt
    11281 tttgcccacg cgctttctca atcaccgtac ccgcagctgc cgggtcaacc aggttgattg
    11341 ccagtgcctt cacccctttc gccagcaata cgtcgatctg atcgttctgc ttggactggt
    11401 cattctgaga atcattcatc agcagctgaa catctggcgc ggctttcgca tcttgctcaa
    11461 tagccttgcg cactacagac ataaagttat cgtcgtactt atagattgtt acaccaatgc
    11521 gagtatcagc agcgtgtgca gcggcaccga ataacatgct ggccatcaca gcagacaggg
    11581 ttaacacctt cttattcatg gtatctccgg tttttcttat gcagggtagt gcttgagata
    11641 aatgctcggc ggggcagtag agttaatgaa gtgttactga acgccgaagc tcacttttta
    11701 aaattcgttc ttccatgccc ggtaacgctc cagaaaacgg ctttaattgt tgttatgacg
    11761 ctattacctc ggtgaaagtg attattcact gttacatacg ggttacaacg ttaaaacggt
    11821 gcaatcatag ccatcacatt gttaagatac tgtgaaatca ctcacagatt gaaagcggtt
    11881 acatcgcctg atttgttgag ttagtgatcg ccgccgcatt ctggcgtgtt gcgacagaat
    11941 ggcgacgcac taacgtcggc ataaaacagt ggctggcacg aggatcaata ttgtctgctg
    12001 ccccctgcaa ggccagttcg gtagctaatt tagccattga agcaatggga taacgcacag
    12061 tcgttaattg cgggtcggtg taacgggcga tgggaatatc atcgaaacca atgattgaga
    12121 gatgtaacgg aatcgcaatg ccattatctt ttaatgctgt cagtgcgcca gcggccatat
    12181 tgtcgttata agcaaataca gcggttagtt gtagattgcg ccccagcagt tcaaccatcg
    12241 ccgcctcacc gcccggcatg tccggcgtgc cagtgccaat ccagctttcc ggcggaataa
    12301 tatcctgctc tttcaacgca ctcatccagc ctgctttacg catggcgtca tcttcaatgc
    12361 cgtggctgga agaaagataa ccaatacgtt gatgaccgtt attcagcaac atgcgcgtcg
    12421 ccattcgggc accgctgaga ttatccaggc aaacgcaacg atgggcgtac cccggcacaa
    12481 cgcggttgat taacaccata ccgggaatgt tatccataaa ttgtgccagt tcatcgtcac
    12541 tcaatgcttt tgagtgaaca atcaacgcat tacaacgctg gcgaattaac acctcaatgg
    12601 cgtgacgctc tttttccgct tcatgatagc tattgccgat tagcacgtat ttctgatgct
    12661 gctgggcgac cagatccacc gcttttacca gcgcgccgaa aaacgcatca gaaacgtcca
    12721 tcaccaccac gccaatggta tcgctaacct gagttgccag cgcctgggca ttggcgtttg
    12781 gccgataatc cagctcactc acagctttca ttactgcttc acgcgtgtcg gcactgacca
    12841 gcgtgctgtt attgagcacc cgggaaaccg ttgccacaga gacgccagcc tgacgcgcta
    12901 catcacgaat ggtgatcata ttcactacct gttatgagat tgcagtaaat gactgcttgc
    12961 tggcggctat tttgtcagca ctgaatacag gacttcgtga atcgagtcac agcaatggaa
    13021 acggttacag ccgttttgtt aacgaatgtg atccagatcg ttatcttgat gttttagata
    13081 ttgtcggtcc ggcagcacgc aaagttaact gacgccagag ccattccacc ggcccctggc
    13141 ggaagtaacg cagccagata acagagaaga agatattcgc caaccatacc ggaataacaa
    13201 acgccagcag ttccagacgg tcaaactgca taaacaaacc gaggtggtaa aaaagcgtag
    13261 tacaaatcag cgtttgcaat agatagttgg tcagtgccat ccgtccgaca caggcgattg
    13321 ccagcaccag cttaaagcgg cttaattgcg gccagaagcc ataaaacagc gacgcatagc
    13381 caatcgcctg aaacggcgca ctcagttccc gcggcatctg aagtaagaag gcgcaccaac
    13441 gatacgccca gtccagtcgc cattgcaggg cgatggcagg aaggttaatg atcaccccaa
    13501 tcgccaccag cacaaaacca gtacgacgat agtgacgtaa gctgaactgc cctttcagcc
    13561 agccactgcg catcaatgcg gcaccaatga gcatcatccc tgccagttgc cagccatact
    13621 gtgcgcccag tgccagtaaa ctgttgccaa caccatcggc acgattactg atcgcttcaa
    13681 caccaccgtg aagtttccag tgcttttcat acaaaatagc cgatgtatca ggtgtccagg
    13741 cgcggctggt ctggctatcg gaaatccccc ccaataacag caaaacgcca agccccacca
    13801 gataaagcat gacgccggta ttaaacaggc tttttaccga tggcgcatcg cgcaccagcc
    13861 gccagcagat taagcccacc agcccgtaag ccagcagaat atcgccgtcc cagaacaata
    13921 aaccgtgaat aaagcccagc aagactaaca gcgttaaccg cgactggatc catcgtctgc
    13981 cacggggcag caacatttgc aggcccgcac caaacaacag cgcaaaaagc gtgaggaatt
    14041 tcacctggcc gatgagatcg agaaatgccc aggtccatgc atcctgtggc gtaatagcgc
    14101 cgtaccaggc gggattgaga taagccgcct ttggtaaccc aaaggcgctg atgtttaata
    14161 gcaggatccc cagaatggcg acgccgcgaa caaaatcgag cgtgacgttg cgctccacgg
    14221 ttcctgcctt tttatcagtt gtgatgacgc acagcgcgca gaaactcgtg gcgcgtattc
    14281 tgactggatt tgaacaatcc accaagagag gtcgttgtcg tggcactggt tgcatcgcgg
    14341 atgccacgcg ccttcacgca gtaatgcacc gcgtcgatag agacagccac gttattggtg
    14401 cccagcagcg tttgtagcgc aatgagaatt tgctgcgtca gacgttcttg tacctgcgga
    14461 cgctgggcaa agaactgcac aatacggtta atttttgaca gaccgatgac cgaatctttc
    14521 gggatatagg ccaccgtcgc tttgccatcg atggtaacaa aatggtgttc acaggtgctg
    14581 gtcagagtga tatcgcgcac ggtgaccatt tcatcgacct tcattttgtt ttcaatgagg
    14641 gtgattttcg ggaaattggc gtaatccaga ccggagaaaa tttcatcgac atacatttta
    14701 gcgatgcgat gcggcgtttc catcaaactg tcatcagcca ggtcgagatt cagcagctgc
    14761 atgatttcgg tcatatgacc agcaataagg cttttgcgcg tttcgttatc catttcatgc
    14821 acgggcgggc gcagcggtgt ttccagtcct cgcgcaacta acgcttcatg aaccagggcc
    14881 gcttctttac tgagtgatgg catttatgat ttctcctgca ggtgtgacgc ctccgccctg
    14941 cgtgggggca aagttattaa gctgatttac agcctgatta ttgtgcgtga ggcggcgcac
    15001 ataatccagt attcacagcg ataattattg taattgccgc tgcctttcat cagcagatgt
    15061 taaaacatcg ttatgcaaat acggaagtga aagttactca cagcacattg aataaacggt
    15121 atgatgaaga aattgcaaac aacacaacaa ggagccacgc atggaaatgc tcgaagagca
    15181 ccgctgtttt gaaggctggc agcaacgctg gcgacacgac tccagtacct taaactgccc
    15241 gatgacgttc agtatctttc tcccaccacc tcgtgatcac accccgccac cagtgctgta
    15301 ctggctttcc ggattaacct gcaatgacga gaacttcacc accaaggcgg gtgcccagcg
    15361 cgtggcggcg gaactgggga ttgtactggt gatgccagac accagcccgc gcggcgaaca
    15421 ggttgccaac gacgatggct acgatttagg ccagggcgcg gggttttatc ttaacgccac
    15481 gcaaccaccg tgggcgacgc attaccggat gtatgattat ctgcgcgatg aattaccggc
    15541 gctggttcag tcgcaattta atgtcagcga ccgctgcgcc attagcggtc attccatggg
    15601 tggtcacggt gcgctgatta tggcgctgaa aaatccgggt aaatacacca gcgtttccgc
    15661 ctttgcgcca attgtgaatc cgtgcagcgt cccgtggaga atcaaagcgt ttagcactta
    15721 tttaggtgag gacaaaaatg catggctgga atgggacagt tgcgcgttga tgtatgccag
    15781 taacgcgcag gatgcaatcc cgacgcttat tgatcagggt gataatgatc agtttcttgc
    15841 tgaccaactg caacctgcag tactggcaga agccgcgcgc cagaaagcgt ggccgatgac
    15901 gctgcgtatt cagccgggat atgatcacag ttactacttc atcgcctctt ttatagagga
    15961 tcacctgcgc ttccatgcgc agtatttact gaagtgaaag tccgcccggt tcgccgggca
    16021 tcttctcatc agaagcgata atccactgcc ataaagtaac gacgtccgtc ttcgttatag
    16081 ctgtagtcgt cacgactgag atccttgtcg ccaaggttca gcacgcctgc gcgcagtttg
    16141 acgtctttag tcacctgcca ggccgcgccg gtattccaga tggtataacc gcccggtgtt
    16201 ttagccgtcg cgctgtcggc gcgtttctgc ccggtatagt gaccagaaac atagaatgac
    16261 cagtcttcca gcgccagcgg tttccagtcc agcgtaccgt tagcagtatg gaacggcaga
    16321 tcggatagcg gtttgttttc gccgttgctg acatcacgac catcgttgta ggtgtagtta
    16381 atcgacagtt tccattcatc gttgaacgga attttcagtt cggtttccac gccctgaata
    16441 cgagctttgt taacgttgta gtagctaaat accggtatgc gccgtccgtt agcgcccgtc
    16501 tcaaaaccga caaagtttta gtagcccggt gcagcattga cgtcagacgt acggctaatg
    16561 ctgatacgat ctttcacatc gttacggaaa acggtaacgc tggattcaac cccttccagc
    16621 cagccttctt cacccatgta gtaaagcccc agctcccaac tttcgctggt ttctggtttc
    16681 agatccgggc tacccacaat cttacatgcg ccacggcagg aattgctcgt ccagtcaggg
    16741 ctaagttgca acagagaagg ggctttaaat gccgtcgccc agcccccttt caccgttacg
    16801 gtgtcggtgg cgttataaac caggtaggca cgcggactcc agtgttcacc gtaggtttcg
    16861 tgatcgtcca tacgcacgcc ggtcgtcagc gccagcggct cgaagatccg ccattcatct
    16921 tccacaaaca gcgcgtactg gctggcagac gttttggagc tggttccccc ggtcaggttc
    16981 accgcatcgc taagtttgtc gtgacgccat tcaccgccaa ccgtgagaaa ctgattaatc
    17041 gccgtcagcg gcaacgtgta tttgccgtcg accgtattgc tttcggaagt tatcgggctg
    17101 ctgttgccag ggtttttgtt ctcgactttc tcaccgtagt atttcagttc gctggtgccg
    17161 taatcccaac gcccattatg gctgacggag tagttctggc gttccaggcg gtttttgtcc
    17221 agcgagtcgg aatcacgatc ctgacggtcg aaaccgtatc cggcagtaaa atcgtgattt
    17281 tgattcggtg tccaggcaaa ttcgacattg ccgtcgcggc tggagaatcc ttcaatacgc
    17341 ggcgtttctc cggtatcggt ggtcgttgag ttttgcgggt cattcttttc acgttttgcc
    17401 aggctgccgt aagctttcat tcccagcacg ccgtcaatta atggtccgct ggtaaagaat
    17461 tgaccgttat aggtatcacc gcgatcgcga tgttcctgaa cggtggtatc gacggtaacg
    17521 gtgcccgacc atttctgacc gatttttttg gtgatgatat tcactacacc gccgagcgca
    17581 tcggaaccgt acagcgacga catcgggcca cgaaccactt caatacgttc gatggaatcg
    17641 accgggatcc agttcagatc gaaatcattg tggcggaaga cggcattgcg ggagttaacg
    17701 cgtttaccgt cgacaagaat cagggtgtag ctgctgtcca gaccacgaat acttacgccc
    17761 ttacggttat ccccttcgtt cgtcagttgt acgccaggca cttctttgag gacatccttc
    17821 agattctgta ccggttttcg ctgcaggtct tcctgggtaa tgacgctgat actggcggga
    17881 gcatctttaa ggttttgttc aacggaagat gcagtgacaa ccatcgtttc gccatcatca
    17941 tcgaccgcta acacaggcca tgcacaagaa atagcggaca aacacagccc gacccgtacg
    18001 aaagggttca acctaaacat tccatatctc catgaggtaa ctacgaaaat aaaatgggta
    18061 tcgctcacat cttctttacg tccccttgcg tacggcagca tcgcggtgga cttatttgta
    18121 tgcaggtgat catcccagaa agccggaaca gcctctgatt tgtcagcctt ttttgattgc
    18181 ggcgtaacga taatgcaaac gataacaatt atcaatccaa atgttaaatt ttatatccgc
    18241 atgatttgtc agggaaatgg aattgaaaaa gccctctcgg ttgagagggc ttagcaagga
    18301 agggaggaaa cttatttctt atcgttctgc gggaacttca tttcgctgta gcgtacgaag
    18361 tgagttcctt taatcagctt gtagccgaac caaataatca ggaacagcgg gataccaata
    18421 tacgttgccg ctacgccgcc ccagtcaatg gtatctttca ggaacgcttc gtagttctgg
    18481 cccaaagtga taatcagaca cagaatgaat gcgaagatcg gccccagtgg gaagaaacct
    18541 gaacggtacg gcagatcgtt aatgtcgtgt ccctgcaata cgtaaccgcg acggaagcga
    18601 tagtggctaa tggcaatccc cagccaggcg ataaaacccg tcatcccgga ggtgttcagc
    18661 agccacaggt ataccgtctg gttgccaaac atggaggtca ggaagcacaa accggcaatc
    18721 accgtcgtcg catacagcgc attacgcggc acgccaccac gcgacagttt agcgaaaatg
    18781 cgcggcgctt taccgtcaca cgccagggta tacagcatac gagtagacgc atacataccg
    18841 gagttacctg ctgacagcac cgccgtcaga ataactgcgt tcatcaccgc cgccgcagag
    18901 agcagacccg cgtgctggaa caccagggtg aacggactga cgctgatgtc tttaacatcg
    18961 ttacgcagca ggctcggatc ggtatacgga ataatcaggc tgataatcag gatcgcgaac
    19021 acatagaaca acaggattcg ccagaatacc tgacgtaccg cgcgtggaat gtttttcgcc
    19081 ggatcttcgg actcgcctgc agcaataccg atcagctcgg ttccctggaa agagaagccg
    19141 acaatcatag ctacgccgat catcgccgca aaaccaccag caaacggcgc ttcgccgatt
    19201 gtccagttgc tccagcctgc aggctgcgcg cctttgaaga taccgataat catcagcacg
    19261 ccaacgatga taaagacaat aactgtcgtg actttgatca gtgagaacca gtattccgct
    19321 tcaccaaagc cgcgaacaga gatgtagttc aacaggaaga taacgccgag gaacaacgca
    19381 ctccagatcc agcccggtgt atccgggaac caccagctca tgaccagctg tgctgcaacc
    19441 aggtcaacgg caatagtcac cgcccagttg taccagtagt tccagcccag cgcgaagcca
    19501 aagccttctt caacatagtt ctgaccgtaa gtggcaaacg aaccggaaac cggcatatat
    19561 gcagccagtt caccgagact ggtcatcagg aagtaaacca tcaggccaat cagcatatac
    19621 gagagcaatg ccccgcccgg acctgcctga gaaatcgttg cgccagaggc aacaaaaaga
    19681 cctgtaccga tggaaccgcc aatggcaatc atcgtcaggt gacgcgcctt caattcacgg
    19741 cgtaagcccg gcgcttctgt agttttagtt tcggaaccca tacaaaaatg ctatccatct
    19801 taaaaatgag gcgcgattgt agcagacgat tggcaatcct tccggcagaa atacacggtt
    19861 ataagacacc ttcatgatcg cccagggatt ataagtaaag cagccaatct cttttctggc
    19921 gaatgcatgc tgagggcgct gcgtcacgaa atcgacacag caccagcatg ttcttgtaca
    19981 gcaccttaac gcggtacatt cgcgggatcg caatagtcca gaaagcgccg tagcgcgttg
    20041 gaaaggtgtt tttgccgatg atgtatacgc cacaacgtac gcatcaggcg cggcagaggg
    20101 accgcaactt cacttaatgt gcctgcctgc aattgatctt caatcacccg tcgcgacagg
    20161 caactaattc ccaacccatg acgcaccgca tgtttgattg cctcggagtt acctaattcc
    20221 atcgccatct caaacttcgg taaatgtgac agcaacagat aatcgacaat ctcccgcgtg
    20281 ccggaaccgc gttcacgcag gatccacggc gcagcggcca gctgttctaa ggtgaccgga
    20341 ccacgagcca acggcgaagt cggcgcggcg aaaaccacca gctcgtcttc cagccacggt
    20401 tcagaaatga tttcagtgct gtggcacggt ccttcaataa agccaatatc aacgcggaaa
    20461 tccagcaccg cttgcatcac gtcctggcta ttcccaacgc taagttcaat cggcaactgc
    20521 ggataatcat ggcgataacg ggcgataact gcaggcagaa tgtagttacc gatggtacta
    20581 ctggcacaga tacgaatcgc gccgttgtct tcgcgaaaca gttgttcgat ttcaaccgcc
    20641 tgttcaagca gtgccaacgc tcgcggatag agcagccgcc cgtgttcatt aacaaccagt
    20701 cttttcccca cgcgatcaaa cagttgaacg ccaagctgcc cttccaggtc ggtcaaggct
    20761 gcgctcactg ctgattgcga caacgccagc atcaccgacg cctgggtggt tgatccactt
    20821 ttcaatactt ctgcaaaaac ttccaactgc cggagggtga tgtgcatagt cgcttaccac
    20881 ttataaagat taattataaa tatataatca attttatttt taaaccagtt agtcgtaacc
    20941 ttatgcccgg ttaaaggaga gggttatgac gaatatcacc ttacagaaac aacatcgtac
    21001 actgtggcat tttattccgg ggttagccct gagtgcagtt atcaccgggg tcgccctgtg
    21061 gggtggttcc attcccgcgg ttgcgggtgc cgggtttagt gccctcaccc tcgcaatctt
    21121 gttggggatg gttttaggca acaccattta cccgcacatc tggaaaagct gtgacggtgg
    21181 tgtgctgttt gccaagcaat atttattacg tctgggtatc attctttatg gcttccgtct
    21241 gacgttctcg caaattgccg atgtcggtat cagtgggatc atcattgacg tgttgacgct
    21301 gtccagtacc ttcctgctgg cttgcttcct ggggcagaaa gtgtttggtc tggataagca
    21361 caccagctgg ttgatcggtg ccggtagcag tatctgtggt gctgccgcgg tactggcgac
    21421 tgagccggta gtgaaagcag aagccagtaa agtaaccgtg gctgttgcaa ccgttgttat
    21481 cttcgggacc gtcgcgattt tcctctaccc ggcgatatat ccgctgatgt ctcaatggtt
    21541 tagtccggaa actttcggta tctacattgg ttctactgtg cacgaagtgg cgcaggtggt
    21601 ggcggcaggt catgccatca gcccggatgc agaaaacgca gcagttattt ccaaaatgct
    21661 gcgcgtgatg atgctggctc cgttcctcat cctgctggcg gcgcgtgtta aacagctgtc
    21721 tggggcgaac agcggcgaga aaagcaaaat cactattccg tggtttgcca tcttgttcat
    21781 cgtcgttgcc atctttaact cgttccacct gttaccgcag agcgtggtga acatgctggt
    21841 aacgctggat accttcctgc tggcaatggc gatggcggcg ctgggtctga ccactcacgt
    21901 cagcgcgctg aaaaaagccg gggcgaaacc tctgctgatg gcactggtac tgtttgcctg
    21961 gctgattgtt ggtggtggtg cgattaacta tgtgattcaa agcgtcatcg cataaaccac
    22021 tacatcttgc tcctgttaac ccgctatcat taccgttttc ctccagcggg tttaacagga
    22081 gtcctcgcat gaaatacatt ggagcgcacg ttagtgctgc tggcggtctg gcaaatgccg
    22141 caattcgcgc cgccgaaatc gacgcaaccg cgtttgcctt gttcaccaaa aaccaacgtc
    22201 agtggcgtgc cgcaccgctc acgacgcaaa ccatcgatga attcaaagcc gcctgtgaaa
    22261 aatatcacta cacatcggcg caaattcttc cccacgacag ttatctgatt aacctcggac
    22321 atccggttgc tgaagctctg gaaaaatcgc gcgatgcctt tatagatgaa atgcagcgtt
    22381 gcgaacagct ggggctttct ttgctcaact tccaccctgg cagccatctg atgcagattt
    22441 cagaagagga ttgccttgcg cgtattgccg aatccatcaa cattgtgctg gataaaactc
    22501 aaggtgtcac cgcggtgatt gaaaacaccg ccggtcaggg cagtaactta gggtttaaat
    22561 tcgaacatct cgcagcgatt atcgacggcg tggaagataa atcccgcgtc ggcgtctgca
    22621 ttgatacctg ccatgctttc gctgccgggt atgatttgcg tactcctgcc gaatgcgaga
    22681 aaacgttcgc tgattttgcc cgtattgttg gctttaagta tctgcgcggg atgcacctca
    22741 acgatgcgaa aagcaccttt ggcagccgcg ttgaccgtca tcatagcctc ggcgaaggca
    22801 atatcggtca tgatgcgttc cgctggatca tgcaagacga ccgtttcgac ggcattccgc
    22861 tgatccttga aaccatcaac ccggatatct gggcagaaga gatcgcctgg ctgaaagcgc
    22921 aacaaactga aaaagcggtg gcctgatcga tgaacgacag ggaaaagcag attcttaaaa
    22981 ttttacggcg taacccgctg attcaacaga atgaaatcgc cgatattctg caaatcagcc
    23041 gctcacgtgt tgccgctcat attatggatt tgatgcgcaa gggtttgatt aaaggcaaag
    23101 gatatatcct taccgagcag gattactgtg tggttgtggg tgcaatcaat atggatattc
    23161 gcggaatggc ggatatccgt tacccgcaag cagcctctca tccaggttct gttcactgtt
    23221 ccgcaggcgg cgtcggacgt aatatcgcgc ataacctggc tctgcttggt cgggatgtgc
    23281 atttaatttc agctattggc aacgattttt acggcgagac attactggaa gaaacgcgcc
    23341 gcgcgggcgt gaacgtttca aactgcattc gtctgcatgg acacagcact gcaacttatc
    23401 tcgctatcgc taataagcag gaagaaacta tcctggcaat caatgacaca catattctgc
    23461 aacagctaac gcctcaactg ctcaatactt cacgggactt gattcgccat gctggtgtgg
    23521 tgctggctga ttgtaatatc acacctgaag ccctggagtg ggtctttacc attgctgatg
    23581 aaattccgat gtttgtcgat accgtttccg aatttaaagc gaacaaagta aaaaactggt
    23641 acagccggat ccacactctg aaaccgacac aaaatgaact ggaaattttg tgggggcaac
    23701 cgattaaaga cgacaacgat cgcatcagag ctgtaaattc gctgcatcaa caaggtgtta
    23761 aacgcatttt tgtttatctg aaagatgaat ctgtattttg cagcgataag gatggagaac
    23821 aatttttgct gaccgcgcca gcgcatacga cagtagacag ttttggtgct gacgatggtt
    23881 ttatggcggg cctggtatat agctttcttg aaggatacag tttccgcgac agcgcccgtt
    23941 ttgcgatagc ctgcgcggca atttcgcgcg ccagcggcag cttaaacaac cctaccctgt
    24001 ctgccgataa cgcgctttca ttagtgccaa tggtgtaaca atgttgccgg attcgacgct
    24061 tgaccgcatc ttatccggct cataaattgc gcactacgct aaaccaataa agaatcctgc
    24121 aatagtagca ctcatcaggt tactgacagc aaaaacgcaa tagccagtaa taccaccatt
    24181 cccagaacac ttctcatgac atccatagtt attatctctt catgtcattt tgggcgacgg
    24241 ctctctgccc atcataaata gccgggcctg catcttgcga tactgacccg gcttgttatc
    24301 aacacttaat gggtttttat gtagccgcgt acgcactctt cgactaatcc ccagaaccag
    24361 tcggtatcaa tagtgatgcc gactttggta ttggcgggct tgccaagaac acccagctcg
    24421 tcgcagacgg tacgcccata gcaagggcca ctgttaacgt ccacttcgac gtacatctcc
    24481 tgggttttaa tgccatcagg gttaatcaaa taaccgatgc aggtggcgtc gtgcaccggg
    24541 ccgccagcca ggccgtagtt ttcgaactgc gttttgagag tgaagttcat gatgtcgctg
    24601 aacaactctc cggcagggcc gccagccctt tctatccgag caatcacatc cggggtgcaa
    24661 acggtctggt tggtaagatc gaggcctatc atcactaatg gaacgccgga ggtgaacacc
    24721 acgcgtgcgg cctccgggtc agcaaagatg ttgaactcag cagatggggt gaagttgcca
    24781 gtaccataag caccgcccat cagaacaatt tcacggattt tcggcaggat cgcgggttgc
    24841 atacgcattg ccaccgcgat gtttgacaac ggaccaaccg gcaccagagt gatatcgcca
    24901 tcgcttgcca taagggtatc gatgatatat ttcaccgcat gagtgttttc tgcctggcgg
    24961 gtcagcggct cgaataccgg gccatccagt ccggtttcac cgtgaatatt atcggcaaca
    25021 atttgttgac gcataatggg ctgcggcatc cccgcataaa ccggaacatt aatctccagt
    25081 ttctggcaaa cattcaggcc attaattaat gttttatcga gggtttgatt acccgctaca
    25141 atggtgatac ctaataaatc aatcgccgga tgtttcgccg ccatcattat agcaatagca
    25201 tcatcatgac ccggatcaca atccagaata atttttctct tttccattgt ttatttcctc
    25261 tgtttccagt tgcgttattg tttctacagc aaagaaaatt cgacctggcc gatgattgaa
    25321 tcttaacaac agcgtacgta tgctaaatat gagaaatctc atagcggata aacatcgtga
    25381 aagaaatcca caataatgat cttaagcagc aattgataag tgaatctgcg tttaaggatt
    25441 gcttttcaac ggatgtttca gccgatacgc ggctgtttca ttttttagcg cgtgactaca
    25501 ttgtgcagga agggcaacag ccgtcctggc tgttttacct gacgcgaggc cgcgccaggc
    25561 tttatgtcac gcttgctaat ggtcgcgtgt cgctgatcga tttctttgcc gcgccctgtt
    25621 ttattggcga gattgagtta atcgacaaag accatgaacc gcgtgcagtg caggctattg
    25681 aagagtgttg gtgccttgcg ctccctatga agcattaccg cccgttgtta ttaaacgaca
    25741 cgctattttt acgaaaactc tgcgtcacct taagtcataa aaattatcgt aatattgttt
    25801 ctttaactca gaatcaatca tttccgttag tgaatcgcct ggcagctttt atattactct
    25861 cgcaggaagg tgatctttat cacgaaaagc atacgcaagc ggcagagtat ttaggcgttt
    25921 cttatcgaca tcttttatat gttcttgcgc aattcattaa tgacggttta ttaattaaaa
    25981 gcaagaaagg gtatctcatt aaaaacagaa agcagttgtc aggactggcg ctggagatgg
    26041 acccggagaa taaattctcc gggatgatgc agtaaaaatt attggcaata gtgcgattgc
    26101 cggatgcaac gcttaataag gcgtacatcc ggcaatgaaa ctcaagctaa accaataaag
    26161 aacccggcaa tggtcgcgct catcaggttg gaaagcgtcg ccgccgccag cgcccgtaaa
    26221 ccaagctggg cgatttccgg cgcgcgtttt ggcgaaatag ccgaaaacgc tccaacgaca
    26281 acaccgatag aaccaaagtt tgcaaaccca cagagggcga acgagataat ggcaatggtc
    26341 ttgacatcca gcgcgccgct cgtttgcaga tagggcgaga aattgagata agcaacaaac
    26401 tcattaatcg ccagtttctg cccaatcaaa cttccggcaa gatttgcatc actccagtcc
    26461 acccccataa tccatgccaa cggggccagc acatagccga aaatgctttc cagagagaca
    26521 ttggcaaaac caaaccagcc gccaactcca ccaataatgc cgttgatcag cgcaatgatc
    26581 gccacaaatg ccattaccac cgtcgccaca ccggcggcga ttttcagccc ggtcattgca
    26641 ccgttcgccg cagcttcaat aatgcttttt ggcggtgttt cgctaaacga cagattttca
    26701 aacgtaactt gcgaaggttc cgtcgccgga ctaagaatac gtgcaaacag gataccgcca
    26761 ggaatagcca tcagcgaagc ggctaacaaa tagtcaatcg gcacgcccat tccggcatag
    26821 ccaatcatca tcgaacccgc aatagacgcc atgccgctac aaatagccgt aaatagctcg
    26881 ttgcgattca tgcgatcaat aaaaggttta acgatcgccg ggatctcatt ttgcccgagg
    26941 aaaatagtgg tgactgcgac aaaagattcg attttgctga tgttgagggc tttctgaaaa
    27001 atactgccaa gaatgcggat cagcagcccc attacgccaa tgtagtacag caagctgata
    27061 agcgcggtaa cgaaaataat cgccgggagt acgcgaaagg cgaagataaa gcccgcaccg
    27121 tcaaacagaa catccatttt gggaccgacc agcgaaccaa aaataaaggc acttcctgcg
    27181 tcactgtagg acatcacttt atggacgcct aatgccgcct gctctactgc ccattttccc
    27241 gggggaaaat agagcatgat gccaccaatg gcaatttgca gcagcagcgc agccccaacc
    27301 gtgcgcaagc tgatgctctt tttatttacc gacaacacaa aggcaattgc cagcaatacc
    27361 accatcccca caacacttct cattatatcc ataatgattt tcccttcatg ccagtaaacc
    27421 cggcgtcagc gccaggtttt ggtatgtttg atgagtacgg gcgacggctt tctgcccacc
    27481 agaaattaac ccacgagacg ctgatattct ttggcaattt cgctcgccag aatggcgtta
    27541 ttaaagacca gttgaatatt ggatttcagg ctatcaccgc cggttagctc cgcaacacgc
    27601 gccagcagga acggtgtact ctctttccca ataacaccct gttcttcggc ttcagcaacg
    27661 gcttgatcaa ttgctgcatt gatggtttct tctggcatcg caaactgttc cgggatcggg
    27721 ttcgctacca ccaggccgcc gttcaaaccg gtttgccact tcactgccat cgcgcgagca
    27781 atttcgcggg cactgtcgag acgaatgctg acttcaaatg ggctggttcg gcagaaaaac
    27841 gcaggcagcg cttttgtctg ataaccaatt aacggtacac cgaaggtttc taaatattct
    27901 gttgttaatc ctaaatcgag aatagatttt gcccccgcac aaacaacagt gacattggta
    27961 tttgccagtt cctgtaaatc ggcagaaata tcgaaggtat gttccgctcc gcgatgcaca
    28021 ccaccaatac ctccggttgc aaaaacttta atcccagcca gtgctgcaat aatcatggtt
    28081 gatgcaacgg tagtcgcgcc atttttccct gcagcaacga caaaaggtaa atcacgacga
    28141 ctaactttcg tcacgttatg tccttcacgg cccagcaatt cgatttcttc tttacttaag
    28201 cccactttca tcacaccgcc aataatggcg atagtggctg gtactgcgct ctgtttacga
    28261 attgtttctt ctacttcaat tgcggtttgg gcattttgtg ggaatggcat tccgtgagaa
    28321 attatggtcg attccagcgc aacaaccggt tttttatttt ttaaagcatc ctgtacttca
    28381 gcggatattt gtaataattc aggggacaga gttaattcag acattctgtg ttctccacta
    28441 acgatgtaac attggcaatt gataattcag ggttgttggt atattcacag gcaagtgcca
    28501 ttgacgaaca tccctgcgca aatcgaacag aatcgataaa cggcataccg tctacccaac
    28561 aggaggccag ccccgccatc atggcgtcgc ctgccccagt aacattgatg acattcgttt
    28621 taataggggc cgaccaccca ctttcaccgt tgatatcact gtagtaaacg ccatcccctc
    28681 ccatgctcag caccaggcgg ttcaggccgt gttgatgaaa ccaggcggcg acctttgcga
    28741 catcttcacg tccggaaagt gcaattccgc tcaacgtttc ggcttcaaga cggttaggtt
    28801 ttaacgtatg gattttgctc agatgatcgc ggattttgac gcatttccat gccgataccg
    28861 gatcaataaa tacaggcgtt tccccgctat tttccagaac ccagaccagc gcctcttcac
    28921 taaggttgca atccgcgaca ataacctttg caccgcgaat aaactcctga tgctgggcaa
    28981 gaaattctac agaaatacag tcactaatac tcatatcatt tatcgcgaca agcatttctc
    29041 ctgtattatc gagcagtgat aaatagctgg atgtattctc acccggaaca attaagcatt
    29101 tatcgacata aacaccggat tgattagttt gcgccagtaa tgattgccca taaaaatcac
    29161 cacctactgc acttaaaagc caggcatttt tccccagtaa cgccagatta tgagcgatat
    29221 tccttcccac gccacctggg gtaaatttaa tttttccggg attcgaatcc gcataattca
    29281 atggatgatg tgaataccca gccacatcaa tattcgccga gccaataatt actatgtagt
    29341 ccttttcgcg catagcacgc cccctggcaa taaattgcca acgaagaaat tgggtaaaat
    29401 agtcattaca gtcaataaga ccattagagc acgtgttcag atttaaacac atgttcataa
    29461 taagcatgtt taacgcaatg taaatcgatc agaaaaaacg aaatcggcca gatatgaagg
    29521 cggtcacaaa atttgtctat ttatgcagca acaaaagaga aagttgtgat gtgtaacaca
    29581 caatatggca ggcaagaaaa agggcagaga tgtttctgcc ctcagattgt tgacaatgcg
    29641 cgctttattc atgctggatg tggcgtaaac gccttatccg acctacaaaa gcgcaaaaat
    29701 tcaatatatt gcagggttca tgtaggtctg ataagcatag cgcatcaggc aattttgcgt
    29761 ttgtcatcat cggggcagag atgtctcagc cccgatgatg aagagaaatt atgcgttttt
    29821 ttctactatt tgtgtttccg ggcgtttgag gaaggcatag gccaaacccg ccaccagcgt
    29881 accagcaata attgctacca ggtaacccaa taccggcgta atagcgccag ggatcagcag
    29941 aacaaacaga ccaccgtgtg gtgccatcag tttcgcacca atcgccattg agattgcgcc
    30001 agtcagcgcc ccaccaacga tacagcacgg tagcacacgc atcggatcac gagcagcaaa
    30061 cggaattgca ccttccgaaa taaagcacag accaagtacc agagcggctt tgccaccttc
    30121 ctgctgcgct ttgtcgaatt tgcgacgcgc caccattgtt gccagaccca ttgccagcgg
    30181 tggcaccata cctgccgcca taatcgccgc catcgggcca taggtttgag tactcagcag
    30241 acccacaccg aatgcgtacg ctgctttatt taccggaccg cccatgtcag tacacatcat
    30301 gccaccgagg atcgccccca gcagaacagc attcgcagtc cccatggtct gcagccagtg
    30361 agtcaaccca tcgagaatgc cagcaaccgg tttaccgatc aggtaaatca tcgccagacc
    30421 gaccaccaga ctggaaatta gcggaatgat caggatcggt ttcagtgcct ccatactctg
    30481 tggcagtttc agttgcgtac ttattaactt cgcaatgtaa cccgccagga agcccgcaat
    30541 aataccgcca atgaagccag aaccggtgct aaccgccagc ataccgccaa tcaggcccgg
    30601 cgtgagaccc ggacgatcgg caatggagaa ggcaatataa cctgccagta ccggcaccat
    30661 cagcgcaaag gctgaaccac caccaatctg catcagcgcc gctgccaacg tacccggctc
    30721 tttaaacgct tcgataccaa aagcaaaaga aagcgcgata cacagaccac ctgcaacaac
    30781 catcggcagc atgtaagaga cgcccgtcag caagtgacgg taagcacctg cactctcttt
    30841 cttaccttca gtggtcgctg tttgagtttt gcccgccggt tcatacggcg ttgcttcagc
    30901 aaccgcttta tccagttcct gcgcagtttt cttcagcgcc agaccggtag aggtgcgata
    30961 catcggttta ccagcaaatt tcgccagatc cacttcgata tctgccgcca caatcaccag
    31021 atccgctgct gcgacttctt caggggtgat tgcattgccc gcgccaacag aaccacgggt
    31081 ttcaactttc acccaccagc cacgtttttt cgcttcggtt tcaatggctt cagccgccat
    31141 aaaggtgtgt gctacgccag tcgggcaagc agtcaccgca actacgcgtt tcggaccgct
    31201 ggcagcaacc ggtgctgtcg cagcgaccgg cgcagtgtaa ggtttcgcat ggcctttggc
    31261 ttcactcagg aacaactcag ggtgcgcaac tgcccgggaa atatcaccca gccagacatt
    31321 tttaccgttc agcgcgctgt cattcgggat ggaatcaccg agaacaatcg ccatttctgc
    31381 ttcgttcgga ttgtcgatga tttccagttt tgcttttcgc gccgccgcgc ccagcagggt
    31441 cttcgccata taggcgcgtg cctgaccgag attagcgtca ataatcagca gcgttttcat
    31501 tatgcctctc ctgctgtcag ttaaaaggtt gtaagtcgac gcgcgccatc attgcggcca
    31561 actgcggacg atcggtaata cccacattgc tttgacttac cgccagggct gcaacagctg
    31621 tcgccagacg cagtgtgtgt tcactggatt cacgcatcag caagccataa atcaggccac
    31681 caaccataga atcccctgcg ccaacggtgc ttacgacatc gactgacggt ggtttggcga
    31741 tccattcgcc ggaggcatta acccaaagcg cgccttcggc acccagtgaa ataacaacat
    31801 gcgcgatgcc ttgttcacgc agcgcatgcg cagcttcaat cacatctttc atttcaggca
    31861 gtttacggcc tgcccagatt tccagctcgc ggcggttagg tttcaccagc cacggtgccg
    31921 ctttcaaacc tgctactaac gcttcacggc tactatcaaa gataatgcaa ggacactgac
    31981 tacgcaggcg agtcatccag tcggtgaacg cttccgggct gacgcctgac ggtaagcttc
    32041 cgctgacaca gaccatatcg aactgaccga gccagctcag agaatcagtc acaaagcgtt
    32101 cccagtcggc gggggtgact tcaaaacccg agaagttgaa gtcggtcact tcgccgtctt
    32161 tttccgtcag cttaacgtta attcgggtgc gcccctgtac aacctggaaa cggttggcaa
    32221 tgcccagctc gctgaacagt tgctgaaaac catcctgatt gtctttaccc aggaagccgc
    32281 caacggtgac atcaattccc aggtctttta atactttggc cacgttgatg cctttacccg
    32341 ccgcatgcag accagtggtt ttcaccaggt tcacttcgcc gcgttcaatt tccgggcaga
    32401 aaccaacaag gtcataagcc ggattaaggg tgatcgtagc aacacgtctg ctcattatgc
    32461 gccctcccca agaccagcag cgatagcgtc gccaatggct ttcagcgcct gttcagcatc
    32521 tgcaccctgg gcggtaaagc gtaggcgatg acctttctta acgccaagtg ccacaacttt
    32581 catcagacta cgtccgtttg ccggtttgcc ggtgccatca aggtttgtca cggtaatatc
    32641 actgttaaat tgtttaatgg tattgaccag catggtacct ggacgagcat gcaggccgtg
    32701 ttcattgcgc accacaaact ccgcgctcaa cacgtcgtcg gtcggcgcat catcgctggt
    32761 cagcagcgcc agcaacgttg ccgcatccgc tttcagcaag cggtcagctt tattatcgag
    32821 caacaaatca gcgagacgct taagaaccgc gatgggctga tcgtcattca tcgccacact
    32881 caccagcatg gctgccgttt cgccgtccac atcaaaagca tttgccgcac ggcttaccgc
    32941 aatcgcgcta cgcagattgc cttcggcgct atcgctcagc cagataccct gcccgaggtt
    33001 cagcggttgt tcattgatgg ctttggtgac gaaagtggcg tcaactgccc ccgcctcttt
    33061 cagacgcgca gcgttcagcg cctgaagagt cagcagatcg ctggcgacga tatccaacgt
    33121 cagcatttcg ttgtcgagct tcagctgctc actctgcttt tcgcccatca gcaatgcgcg
    33181 aagttcttct gctgttgttg ctgacttcag ttgttcagca acggaatcat cgctcagtac
    33241 gtgggtcagc tggcgtagca ggcccagatg ttcatccgag ctggcagcaa taccgatcgc
    33301 cacgtacgct acctggccgt caccccaggt gacgccttcc gggaactgaa atacctgaac
    33361 gccggttttc agcacctgat cgcgggtgtc ggtagtgccg tgtggaatag caataccatt
    33421 gccgaggaac gttgaggttt gctgttcgcg cgccagcatg ccattgacgt agccttcagc
    33481 tacattaccg gcctgcacca gcgccgcagc gacctggcga atcgcctctt ctttgtctcc
    33541 ggccttttcg cccggatgga tgtcctgtac ggataactgg aacatagttc tcctctcttg
    33601 ctgaattgaa acgattcagc ttctatgaga aaaaagcgcc aacctggctt agggttaaag
    33661 acaagatcgc gctgaaacgt ttcaagaaag cataatactt ttgtttcagc acgcaaggaa
    33721 agccgtaaag ttatgagcaa aagtttgatg tgctgcacaa ttttttgtca tttttcctaa
    33781 ttgctgatgg gaaaatccgt tatcagcaat ttcatttcag cagcgtcagc agccaatcag
    33841 caacagtgaa atggcatttt gattattcgt ggcgcttttg atttcgctgt ctgtttattt
    33901 tctgacaagc agcgtaaact ccgcgtcttc ctcttccagt gatcgaccag catgcataac
    33961 tcccccgcag tcaccagcgc gaaatcgttt gacctgacct cgatggcgtt tttaatcgtt
    34021 gcctttctca ccggtattgc gggcgctctg caaaccccga cactcagtat ttttcttact
    34081 gatgaagtac atgcccgtcc ggcgatggtg ggattcttct ttaccggcag cgcagtcatt
    34141 gggattctgg tgagtcagtt tctcgccggg cgctctgata agcgcggcga tcgcaaatcg
    34201 ctgattgtct tttgctgcct gttaggcgtg ctggcctgca ccctttttgc ctggaatcgc
    34261 aactactttg ttttgctatt cgttggcgtc tttcttagca gctttggctc aaccgctaac
    34321 ccgcaaatgt ttgcccttgc ccgtgaacat gccgacaaaa ccggacgtga ggcggtgatg
    34381 ttcagctctt ttttacgcgc tcaggtttca ctggcatggg tcattggccc accgctggct
    34441 tatgccttag cgatgggttt cagctttacg gtaatgtatc tgagcgcagc ggtagcgttt
    34501 attgtttgcg gcgtgatggt gtggctgttt ttaccgtcga tgcaaaaaga gcttccgctg
    34561 gcgaccggca cggttgaagc gccgcgccgt aaccgtcgcg atacgttgct gctgtttgtc
    34621 atttgtacat tgatgtgggg ctcgaacagc ctgtacatca tcaacatgcc gctatttatt
    34681 atcaacgaac tgcatcttcc cgagaaactg gccggtgtga tgatggggac cgccgccggg
    34741 ctggaaatcc cgaccatgtt gattgccgga tattttgcca aacgtctggg taagcgcttc
    34801 ttaatgcgcg ttgctgccgt gggtggcgtc tgtttttacg caggaatgct gatggcgcat
    34861 tcacctgtca ttctgttggg cttgcagctg ctaaatgcta tttttattgg cattctgggc
    34921 ggtatcggga tgctctattt tcaggatttg atgcccggtc aggcaggttc agccaccacg
    34981 ctctatacca acacgtcgcg cgtgggctgg atcatcgcag gatcagtggc gggcatcgtc
    35041 gccgagatct ggaattatca cgctgtgttc tggtttgcga tggtgatgat tatcgccact
    35101 ctgttttgct tactgcgaat taaagatgtt taaggcgcgg tcaacatttc cagatcgata
    35161 agccaggtca ttgcctgctg acgcgagtta ccgcacattt cagcgctggc ttgtaagcct
    35221 gcacagactt tcggtcgcag tggcgaggtg aaaattttac agcgttgctg ttcatcaagc
    35281 tgaatgcagg gcgtattggc gggcttgcca tctggcatac cgggaatagg gctggaaatt
    35341 gaaggggcag tgcaacacgc cccgcaaccc ggacggcatt ccatacgttg ttctcttaag
    35401 gttaaccacc ggattgatga gcgcgcacag taccaccttt tttgcaccag caaaagtgcg
    35461 aataccactt gcccgaaaga cccgtcgcga gtactttgtc gcgatatttt tgacattttc
    35521 gactacagga atttttcgat gccaagagcg aacgaaatta aaaaaggtat ggtactgaat
    35581 tacaacggca aactgctgct ggttaaggac attgatattc agtcgcccac tgcccgcggt
    35641 gccgctacgc tgtacaaaat gcgtttttct gatgtccgta ccgggctgaa agtagaagag
    35701 cgtttcaaag gtgatgatat cgttgacacc gtgacgctga cccgccgtta cgttgacttc
    35761 tcctatgtcg atggtaacga atatgtcttt atggataaag aagactatac cccgtatacc
    35821 ttcaccaaag atcagattga agaagagttg ctgtttatgc ctgagggcgg catgccggac
    35881 atgcaggtgc tgacctggga cggtcaattg ctggcgcttg agctgccgca gaccgttgat
    35941 ctggaaatcg ttgaaactgc accaggcatc aaaggggcat ctgccagcgc ccgtaacaaa
    36001 ccggcgacat tgagcactgg tctggtgatt caggtaccag aatacttaag cccgggcgaa
    36061 aaaattcgta tccatatcga agaacgccgt tatatgggtc gtgctgacta acttcagccg
    36121 catgcagaaa agggatagct caggctgtcc cttttttaat ttattatacc aatcttctat
    36181 tttgcgcttc ctgaacacct tacgcccgca attttcactc gttgattgat cgccctcaca
    36241 cttcatcgca ttaacaatcc agaccaattt caattgttgt catataactt tacactgtca
    36301 ttgttaatta atcgttacta agacgtgact cctatgaata caatcgcctc cgttacgctc
    36361 ccgcatcatg tacacgctcc acgctacgat cgacagcagt tgcaatcacg tatcgttcat
    36421 tttggctttg gagcctttca ccgcgcacat caggcattac tgaccgatcg tgtgctgaat
    36481 gcccagggcg gcgactgggg gatctgtgaa atcagcttgt tcagcggcga tcaactgatg
    36541 agccaactcc gcgcacagaa ccatttatat accgtgctgg agaaaggtgc ggacggcaat
    36601 caggcgataa ttgtcggtgc cgttcacgaa tgtctgaatg caaaactgga ttccttagcg
    36661 gcaattattg agaaattttg cgagccacag gtggcaattg tttccctgac gattaccgaa
    36721 aaaggctatt gtattgaccc ggccaccggt gcactcgaca ccagcaatcc gcgtattatt
    36781 cacgatctac aaaaccctga agaacctcac tccgcgccag gtattctcgt cgaagcactg
    36841 aaacgccgcc gtgagcgcgg ccttacaccg tttaccgtgc tctcctgcga caatattccc
    36901 aacaatggcc atgtggtgaa aaacgcggtg ctgggaatgg cagaaaaacg ttcgccagaa
    36961 ctcgccgggt ggataaaaga acacgtcagt tttccgggaa ccatggtcga ccgcattgtt
    37021 ccggctgcaa ccaacgaatc actggcggaa atcagccagc atctgggggt gaatgatccc
    37081 tgcgcgatta gctgcgaacc gtttatccag tgggtggttg aagataactt cgtcgctggg
    37141 cgtcctgcct gggaagtcgc aggtgtacaa atggtgaatg atgtcctgcc atgggaagag
    37201 atgaaactgc gtatgcttaa tggcagccac tcttttctcg cttatctggg ttacctctca
    37261 ggattcgccc atatcagtga ttgcatgcag gatcgcgcat ttcgccatgc cgccagaaca
    37321 ttaatgctgg atgagcaagc gccgacactg cggattaaag atgtcgattt aacacaatat
    37381 gcggataagt taattgcacg ttttgctaat ccggcactga aacataagac ctgacaaatc
    37441 gcgatggatg gcagccagaa attaccgcaa cgcatgttgg caggtattcg catacatctg
    37501 gggcgcgaaa cggactggtc gttgctggca ttaggcgttg caggctggat gcgttacgtc
    37561 agcggcgttg atgatgccgg aaatgccatt gatgttcgcg atccgcttag cgataaaatt
    37621 cgtgaacttg ttgcggtcag cagcagtgaa caacgcgtaa ccgccctgct ttccctgcgt
    37681 gaaattttcg gtgatgatct gccagataac ccgcattttg tgcaggccat cgaacaagcc
    37741 tggcaacaaa tcgcacaatt cggcgcgcat caggcgctat taaacaccct caaaatttaa
    37801 cgatttctgc ggttaaagcg gatgaagctc gccttcgtcc gctctcccct tctcttttct
    37861 gcctttttta gccaggatta acgctcagtt aacttaccag agtattgcgg agccgttgtg
    37921 accagaacca acctcatcac cggttttctc ggcagcggga aaactacgtc gattcttcat
    37981 ctgttagccc ataaagatcc caacgaaaaa tgggcggtac tggttaatga atttggggaa
    38041 gtcggaattg atggtgcttt gctcgccgat agcggcgcac tgctgaaaga gatccccggc
    38101 ggttgcatgt gctgcgttaa tggtttaccc atgcaggtag ggttgaatac cttactgcgt
    38161 cagggaaaac cagaccgctt gttgatagag ccgaccgggc tgggccatcc gaaacagatc
    38221 ctcgatctct taaccgcacc cgtctatgaa ccgtggatag atctgcgcgc cacgttgtgc
    38281 attctcgatc cacgcctgtt gctggacgaa aaaagcgcca gcaatgaaaa cttccgtgac
    38341 cagctggctg ccgcagacat tattgtcgcc aataaatccg accgtgcgac gcccgaaagt
    38401 gagcaagcgc tagagcgctg gtggcagcaa aatggtggcg atcggcaatt aattcacagc
    38461 gagcatggaa aaattgacgg tcatcttctg gatttgccgc gtcgcaattt agccgagttg
    38521 cccgccagcg ccgcgcattc tcatcagtat agcgtgaaaa aagggttagc agcgttaagc
    38581 ctgccagagc atcaacgctg gcgtcgcagt ctgaacagcg gacaagggca tcaggcctgt
    38641 ggctggatat tcgatgctga tacggtattc aacaccattg gtattctgga atgggcgcga
    38701 cttgcaccgg tggaacgcgt caaaggcgtg ctgcgtattc ccgaagggct ggtgcgaatc
    38761 aaccgtcagg gcgatgacct gcacattgaa acgcaaaacg ttgcgccacc ggacagtcgt
    38821 attgagctga tttccagcag cgaagctgac tggaatgcct tgcagagcgc gctgttgaag
    38881 cttcgtttag cgactaccgc gtaaggttgc ctgcgttttt cagtaagata attagagaaa
    38941 atatgattaa aaatttgccg caaatagtgt tgttgaatat tgtcggcctc gcgctgtttc
    39001 tttcctggta tattcccatt aatcatggat tctggttgcc gattgatgcg gatatttttt
    39061 atttctttaa tcagaaactg gtcgaaagta aggccttttt atggctggtt gcattgacca
    39121 acaatcgcgc tttcgacggt tgttcactgc tggcgatggg aatgttgatg ctgagtttct
    39181 ggctgaaaga aaacgcccca ggcagacgac gtatcgtgat tattggtctg gtcatgctat
    39241 taactgcagt ggtattaaac cagctgggtc aggcactaat tcctgtaaaa cgggccagcc
    39301 caacgttgac ttttaccaat attaaccgcg tcagcgaact gctctctgtt cccacgaaag
    39361 atgcctcacg agatagcttt cccggcgatc acggtatgat gctgcttatt ttttcggcat
    39421 tcatgtggcg ttatttcggc aaagttgcag gccttatcgc ccttattatt tttgtggttt
    39481 ttgcgtttcc cagagtaatg attggcgcac actggtttac tgacatcatc gtcggttcca
    39541 tgaccgtgat attgatcggt ttgccctggg tgttgctgac gccattaagt gatcgattaa
    39601 tcaccttttt tgacaaatca cttcctggaa aaaacaaaca tttccaaaac aaataactca
    39661 cagtaattaa catcatcagg tttattttta tagtgaggat aatcctgatg atgcgcctcc
    39721 tgctttcatc catcgagcgc aaaaatcatt ctctaagtaa atgaatggat tgcatgcgtt
    39781 tcactcaatc gtactttaat tgaccaatcc cgctgattaa ctttctgtat cactttttct
    39841 tataaaaaat catgtaaaac cgctcgccaa gaccgcacca atcgggtaat ctcgaactcg
    39901 ttttgcctcg gcggtagatt atcctcacag catataattt tgtgcgttag tccacagatt
    39961 tggccttaag gaattgtttc aacatgccca ggtaattagt ctcgtgtcgc ttggcatttt
    40021 tttataacga tatttgtcgt taaggacttc aagggaaaac aaacaacatg gtcaaatctc
    40081 aaccgatttt gagatatatc ttgcgcggga tccccgcgat tgcagtagcg gttctgcttt
    40141 ctgcatgtag tgcaaataac accgcaaaga atatgcatcc tgagacacgt gcagtgggta
    40201 gtgaaacatc atcactgcaa gcttctcagg atgaatttga aaacctggtt cgtaatgtcg
    40261 acgtaaaatc gcgaattatg gatcagtatg ctgactggaa aggcgtacgc tatcgtctgg
    40321 gcggcagcac taaaaaaggt atcgattgtt ctggtttcgt acagcgtaca ttccgtgagc
    40381 aatttggctt agaacttccg cgttcgactt atgaacagca ggaaatgggt aaatctgttt
    40441 cccgcagtaa tttgcgtacg ggtgatttag ttctgttccg tgccggttca acgggacgcc
    40501 atgtcggtat ttatatcggc aacaaccagt ttgtccatgc ttccaccagc agtggtgtta
    40561 ttatttccag catgaatgaa ccatactgga agaagcgtta caacgaagca cgccgggttc
    40621 tcagccgcag ctaataaacc gtttggatgc aatcccttgg ctatcctgac gagttaactg
    40681 aaagcactgc ttaggcagtg cttttttgtt ttcattcatc agagaaaatg atgtttccgc
    40741 gtcttgatcc aggctatagt ccggtcattg ttatctttta aatgttgtcg taatttcagg
    40801 aaattaacgg aatcatgttt atacgcgctc ccaattctgg acgtaagctc ctgcttacct
    40861 gcattgttgc aggcgtgatg attgcgatac tggtgagctg ccttcagttt ttagtggcct
    40921 ggcataagca cgaagtcaaa tacgacacac tgattaccga cgtacaaaag tatctcgata
    40981 cctattttgc cgaccttaaa tccactactg accggctcca gccgctgacc ttagatacct
    41041 gccagcaggc taaccccgaa ctgaccgccc gcgcggcgtt tagcatgaat gttcgtacgt
    41101 ttgtgctggt aaaagataaa aaaacattct gttcatctgc aactggtgag atggacattc
    41161 cactcaatga attgattccg gcgctcgaca ttaataaaaa cgtcgatatg gcgatcttac
    41221 ccggcacgcc gatggtgccg aacaaacccg caatcgtcat ctggtatcgc aaccctttgc
    41281 tgaaaaatag cggcgtgttt gccgctctga atctcaacct gacgccttca ctcttttata
    41341 gttcacggca ggaagattac gatggcctcg ccctcattat tggtaatact gcgctatcta
    41401 ccttttcttc acgtttgatg aacgttaatg aattaaccga catgccggtc cgtgaaacta
    41461 aaattgcggg cattcctctg accgttcggc tttatgcaga tgactggaca tggaacgatg
    41521 tgtggtacgc atttttactg ggtggcatga gtggaacttt cgttggactt ctctgctatt
    41581 acctgatgag tgtgcgtatg cgcccaggca gagaaatcat gaccgccatc aagcgcgaac
    41641 aattttacgt ggtgtatcaa ccggtggttg atacacaagc tttgcgggta acgggcctgg
    41701 aagtactgct acgctggcgg catccagtag caggagaaat tcccccggat gccttcatta
    41761 actttgccga agcgcaaaag atgattgtgc cactgactca gcacctgttt gagttgattg
    41821 cccgcgatgc cgcagaatta gaaaaagtac tgccggtagg cgtcaaattt ggcattaaca
    41881 ttgcgccggc ccacttgcac agcgaaagct ttaaagcaga tatccagaaa ctgctcactt
    41941 ccctgcccgc acaccatttc cagattgtgc tggaaattac cgagcgcgat atgctgaaag
    42001 agcgagaagc cacacaactc ttcgcctggc tgcactcggt cggcgtagaa attgctattg
    42061 atgacttcgg caccgggcac agcgcgctta tctatcttga gcgttttacg ctcgattatc
    42121 tgaaaattga tcgtggattt atcaacgcca tcggtacgga aacgatcact tcacccgtac
    42181 ttgacgcggt gctgacgctg gcgaaacgtc tcaatatgct gacagttgct gaaggggtcg
    42241 aaacgccaga acaggcacga tggctaagcg aacgcggcgt taatttcatg caaggctact
    42301 ggattagtcg cccgttaccg ctggacgatt ttgttcgctg gctgaagaaa ccgtatacgc
    42361 cgtagtggta aggtgtgctt acgtccctta ttattcatag tgaaagcatg ccggattgcg
    42421 gctaatgatg agtaaaagga aatccgttgc agatgattgt gcgcatactg ctgctgttta
    42481 tcgctctgtt cacctttggt gcgcaggcgc aggctatcaa ggaaagctat gcctttgccg
    42541 tactgggcga accccggtac gcatttaatt tcaaccattt tgattatgtg aaccccgccg
    42601 cgccaaaagg tgggcaaata acgttgtctg ccctcggcac cttcgataat ttcaaccgct
    42661 atgcactgcg cggcaatccg ggcgcacgca ccgagcagct gtacgacacg ctatttacga
    42721 cttccgatga cgaaccaggc agttattacc cgctgattgc tgaaagcgca cgctatgctg
    42781 acgattattc ctgggtggaa gtcgctatta atccacgcgc ccgttttcat gatggttcgc
    42841 ccattactgc ccgcgatgta gagtttactt ttcaaaaatt tatgaccgaa ggcttgccgc
    42901 aatttcgtct ggtctacaaa ggcaccaccg tcaaagccat tgcgccgtta accgtgcgca
    42961 ttgagttagc taaacccggc aaagaagata tgctgagtct gttttcgctg ccggtatttc
    43021 cagaaaagta ctcgaaagat cacaaactta gcgacccgct cgccacgcct ccgcttgcca
    43081 gtggtccgta ccgcattacg tactggaaaa tggggcaaaa tattgtctat tcccgcgtga
    43141 aagattactg ggcagcaaac ttaccggtaa accgtggacg ctggaatttc gacaccattc
    43201 gctacgatta ttacctcgat gataatgtcg cctttgaagc gtttaaagca ggtgcctttg
    43261 atttgcgtat ggaaaacgac gccaaaaact gggccacgcg ctataccggt aaaaatttcg
    43321 ataaaaaata catcatcaaa gatgagcaaa agaacgaatc agcccaggat acgcgctggc
    43381 tggcgtttaa tatccaacgt ccggtattca gcgatcgccg ggtgcgggaa gcgatcactc
    43441 tcgcctttga ctttgaatgg atgaacaaag cgttgtttta caatgcctgg agtcgcacga
    43501 acagttattt tcagaatacc gaatatgcgg ccagaaatta ccccgacgcc gcggaactgg
    43561 tgcttctggc accaatgaaa aaagatctac cgccagaagt cttcacgcaa atctaccagc
    43621 cgccggtatc caaaggcgat ggctacgatc gtgacaacct gttaaaagcc gacaaacttc
    43681 tcaacgaagc aggctgggtg ctgaagggtc agcaacgcgt taatgtcaca acgggtcagc
    43741 cactcagctt tgaattattg cttcccgcaa gcagcaatag tcagtgggta ttgccgttcc
    43801 agcacagcct gcaacggctg ggtatcaaca tggacattcg caaggtggat aactcgcaaa
    43861 tcaccaaccg catgcgcagt cgcgactatg acatgatgcc gcgcgtatgg cgggcgatgc
    43921 cgtggcccag ttccgattta caaatttcct ggtcatcgga atatatcaat tccacttata
    43981 atgcccccgg cgtgcaaagc ccggttatcg actcgctgat caaccaaatt attgccgcgc
    44041 agggaaataa agaaaaatta ctgccgttgg gacgagcact ggatcgcgta ttaacgtgga
    44101 attattacat gctgccaatg tggtacatgg cggaagaccg tctcgcctgg tgggataaat
    44161 tctcccaacc cgctgtacgc cctgtttaca gcctgggtat tgatacttgg tggtatgacg
    44221 ttaataaagc gacgaaactg ccgtcagcca gacaacaggg agagtagatg ggcgcttatc
    44281 tgattcgccg tctgttgctg gtgatcccaa cgttatgggc gattatcact atcaactttt
    44341 tcatcgtgca aattgcgcct ggcggtccgg tcgaccaggc catcgccgcc attgagtttg
    44401 gtaatgccgg agtattaccc ggcgcaggcg gtgaaggtgt tcgcgccagc catgcgcaaa
    44461 cgggcgtcgg caatatcagc gacagtaatt accgtggcgg acgcggatta gatccagaag
    44521 tgatcgccga gatcactcat cgctacggtt tcgataagcc gatccacgaa cgttacttca
    44581 aaatgctctg ggactacatc cgttttgatt ttggcgacag cctttttcgc agcgcctcgg
    44641 tgctgacgct gattaaagac agtctgccgg tctccatcac cctcggattg tggagcacgc
    44701 tgattatcta tctggtgtcg attccgttag gcattcgcaa agctgtttat aatgggagcc
    44761 gttttgacgt ctggagtagt gcatttatca tcatcggcta cgccattccg gcctttttgt
    44821 ttgccatcct gctgattgtc ttcttcgcgg gcggcagcta tttcgacctg ttccctctgc
    44881 gcggcctggt ttccgctaac tttgattcgc tgccgtggta tcagaaaatc accgattatc
    44941 tgtggcatat cacgctgccg gtgctggcga cagtgattgg cggctttgcg gctctgacca
    45001 tgctgacaaa aaactcattc cttgatgaag tgcgtaagca atacgtggtg acggcacgtg
    45061 cgaaaggggt aagtgaaaaa aatattctct ggaaacatgt gttccgcaac gccatgctgc
    45121 tggtgattgc cggttttccg gcgacgttta tcagcatgtt ttttaccggc tcgctgctga
    45181 ttgaggtgat gttttcactc aatggtcttg gcttactggg ctacgaagcg accgtctcgc
    45241 gcgattatcc tgtaatgttt ggtaccttgt atattttcac cctgattggc ctgctgctga
    45301 atattgtcag tgatatcagc tatacgctgg tcgatccgcg tatagacttt gagggacgtt
    45361 aatgtcgcga ctcagccccg tcaatcaggc ccgttgggcg cgttttcgcc ataaccgtcg
    45421 cggctactgg tcgttatgga ttttcctcct cttgtttggt ttgagtttgt gttctgaact
    45481 tatcgccaac gataaaccgt tgctggtgcg ttatgacggc agttggtatt tcccgttgtt
    45541 gaaaaactac agcgaaagcg attttggcgg cccgctggca agtcaggctg attatcagga
    45601 cccgtggctg aaacaacgac tggaaaataa cggctgggta ctgtgggcac cgattcgctt
    45661 tggtgctacc agtatcaact ttgctaccga taagcccttc ccttctccac cctcccggca
    45721 aaactggctg ggaacggatg ccaatggcgg cgatgtgctg gcgcgcattc tctatggcac
    45781 gcggatctca gttctgtttg gcctgatgct gaccctctgc tccagcgtga tgggcgtgct
    45841 ggcgggggcg ctacaaggct attacggtgg taaagtcgat ctctgggggc aacgttttat
    45901 tgaagtatgg tcggggatgc cgacgctgtt tttgattatt ttactttcca gcgtcgtaca
    45961 gcctaacttc tggtggctgc tggcaattac cgtcttgttt ggctggatga gtctggtcgg
    46021 cgtggtgcgg gcggagtttt tacgtacccg taatttcgac tacatccgcg cggcgcaggc
    46081 gcttggcgtc agcgatcgca gtatcatcct gcgtcatatg ttgccaaatg ccatggtcgc
    46141 taccctcact tttttaccgt ttattttatg tagttcgata accaccctga cctcactcga
    46201 tttcctcggc ttcggtctgc cgctcggttc accgtcactc ggcgaactgc tgttacaagg
    46261 gaaaaataac cttcaggccc cgtggcttgg gatcaccgcc ttcttgtcgg tggcgatatt
    46321 gttgtctttg ctgatcttta ttggtgaagc cgtccgcgac gcatttgatc ctaataaggc
    46381 ggtgtagcat gacgcaaact ctgttatcga ttgaaaattt gtcggtgggt tttcgccatc
    46441 agcaaaccgt acgtacagta gtaaatgatg tttcactaca gattgaggct ggcgaaacgc
    46501 tggcgctggt aggtgagtct ggttcaggta agagcgttac cgcgctgtca attttacgcc
    46561 tgctcccttc cccgccggtt gaatatctct ccggcgatat tcgttttcat ggcgaatcgc
    46621 tgcttcatgc cagcgatcaa acgttacgcg gtgtacgcgg taataagatt gccatgattt
    46681 ttcaggaacc gatggtgtcg ttaaatccat tgcataccct ggaaaaacag ctttatgaag
    46741 tgctttcact ccaccgcggg atgcgtcggg aagcggctcg tggcgaaatt cttaactgcc
    46801 ttgatcgcgt cggtatccgc caggcggcaa aacggctgac agattatccg catcagctct
    46861 ccggcggcga acgccagcgg gtgatgattg cgatggcgct gttaacgcga ccggaattat
    46921 taattgccga tgaaccaacc accgcgctgg acgtctctgt ccaggcgcag attttacagc
    46981 tgttacgcga actgcaaggc gagctgaata tgggtatgct gtttattact cataacctca
    47041 gcattgtcag aaaactggcc caccgcgtgg cggtaatgca aaacggtcgc tgtgtcgagc
    47101 aaaataacgc cgctacgcta tttgcctcac ccactcatcc ttacacacaa aagctactca
    47161 acagtgaacc gtcaggcgac ccggtcccat tgccagaacc tgcctcaacg ttgctagatg
    47221 ttgaacagct tcaggttgcc ttccccattc gcaaagggat tttgaagcgc attgtggatc
    47281 atcatgtggt ggtgaaaaac atcagtttta cgctacgggc gggtgaaaca ctgggtttag
    47341 tgggcgagtc cggttccggg aaaagcacga cgggactggc gctgctgcga ctgattaatt
    47401 ctcatggcag catcgtcttt gacggtcagc cactgcaaaa tttaaatcgc cgccagctgt
    47461 tacctattcg tcatcgcatt caggtggtat ttcaggatcc aaactcctca ctcaacccac
    47521 gactcaacgt tttgcaaatt attgaggaag gcttacgggt tcaccagccg acgctttctg
    47581 ccgcacaacg cgaacaacaa gtgatagccg tgatgcatga agtgggatta gatcctgaaa
    47641 cacgccaccg ttatccggcg gagttctctg gtggtcagcg gcaacgtatt gcgattgcca
    47701 gggcgttaat tcttaagccc tcgctgatca ttcttgatga accaacatca tcactcgaca
    47761 aaacggtgca ggcgcaaata ttgacgctat tgaaatcatt gcaacaaaag catcaactgg
    47821 cctatttgtt tatcagccac gatttgcacg ttgtccgcgc gttatgtcat caggttatcg
    47881 tactgcgaca aggggaagta gtggaacaag gaccgtgcgc gcgcgtgttt gccgcatcgc
    47941 agcaggaata tacgcgtcag ctactggcgt tgagctgacg cttaaaaagg attgtagttt
    48001 gaaaagggtt cggcaattgc cacaccaaaa tttttcaatc ggcatgttgc agcaaattcg
    48061 tcctggcgat ttacaaacag gcacggctca ccttcacact ccagcaccga acatggaact
    48121 tcgatgtcgc ttaacgcctg gcttagtttg tacatcaccg accatgcatt atcaccatcc
    48181 gggctaagca atttaagcgc caccaggctg ttgtcaccgc acggtccatt ttgcggaatc
    48241 ggttcaacct tcgaacctgc gaaaccagca gaccagcgat agttctgcgg cagtcgatgg
    48301 acgattgaga gttgtaatga agtcacttat tttccccgga agtacatttt acttcacaat
    48361 ttgtttacat caattttaac acatcatcaa caatccgtct ttatacagat cgtaaagatt
    48421 gatgcctcgc ctgcgtggcc tctatggctc tgatttaaat aatatctgcg ggcgtggtca
    48481 cgtttgcggg gctatatgta cccgtttctg cgatctaact caaccttttt aactacaatg
    48541 atgtgacttt ttacataaat tgattttaca taaaacaaac atatatcggg ggaatgatag
    48601 atttgtggtt gacatgcatc aacaaaggaa gccttttagc ttcctcgttg tgcaatagat
    48661 caccgttttt tcggccgact ggcgtacaaa cagaagagaa tggagctggt tgcgcagaat
    48721 gcaattgacc aaatcatcgg ccatgcagag ttaaaggtcg caagagaaag taatgcgcca
    48781 acaattgccc ctatgccgaa acggaaggtt cctgccagcg aagatgccgt tcccgccata
    48841 tggggaaact catcaagaat gaccgccatc gcattggatg acaccatcga cacgcagccc
    48901 acaaacgccg caacgccaac caccagcgac cagaatccca gccccagcag tgcactgatg
    48961 accatccacg cagccataat aaactgtatc cacaaccccg agcggaacat atttaacgcg
    49021 ccaatacggc ggacgaagcg gctgttaaag atggtcatca cgaacagaaa aagaatgttt
    49081 agcgcaaagt aataaccaaa gttttccggc gcgacgtggt tgatttcaat gtagacaaac
    49141 ggtccggcgc ttaagaatga gaacatcccg gcaaagctga aaccgctggc cagcatataa
    49201 ctcaggacac gcttatgacg gaacagcgcc gcaaagttac caatagtggt acgaatgtga
    49261 aaaggctggc gacgctccgg cggtaaggtt tctttgatca gaaagaagat cattgccgaa
    49321 gccagaatcg ccgccacggc gaggatccag aagatgtaat gccagctcag ccacaccagc
    49381 acccagccgc caactatcgg tgccatcagc ggtgcaatgg tcgtcaccag catgacaaac
    49441 gacatcatcc gtgagaactc ttctttcggg tagatatcac gcatcaaagc gttgataacc
    49501 acgctggccg cagccgcagc cagcccgtgg aagaaacgca tcacaatcag ctgatcgatg
    49561 gtttgcgcca acgcacacgc caccgcggcg gcggcaaaca ccagcgtacc gccaagaacc
    49621 accggcttac gcccgaagct gtctgccatc ggcccgtaga ttaactgccc caacgcaaag
    49681 cccagaatat aagtactcag ggtcatctgc gtactgcccg ccggtacacc aaactgcgct
    49741 gaaattaccg gtagcgcagg cagatacata tcaatcgaca gcggcatcaa catggccagc
    49801 aggccaagga taaaaacaat agcaaacgac gaatgctgtc gggtggtcac aacgggctcc
    49861 tgaaagtcat tgaaaagtta gatgacgctg gcaatttctt cttcagttaa cggacgatat
    49921 tcaccggggg ctaaatcagc atccagcgta atgccgccaa tacgttcacg atgcagctca
    49981 accacatggt tacccacggc ggcgaacatg cgtttcacct gatgataacg cccttcgctg
    50041 atggtcagac gaacctgcgt tggggtaatc acttccagca ccgccggctt agtgagatct
    50101 ttttcgttat gcagctgcac gcctttagca aattgctctg ccgtatcgtc agctacgggt
    50161 gattccagcg tcaccagata ggtcttctcg caatgatggc gcggggaagt aatgcggtgc
    50221 gaccactgac catcatctgt cattagtacc agaccggtgg tatcgatatc cagacgccct
    50281 gcagcgtgca gtttccacgc taccggttca tcaagaaaat agagcaccgt tgggtgatcc
    50341 gggtcgtccg tggagcaaac atagccctga ggcttattga gcatgaagta acgtggaccg
    50401 tgttgctgcg ccagcgggtt gccatcgtaa gcgacatcat gttcagggag cagtttgaac
    50461 gctgcattac ggacgatttc gccatcgacg gtgacacgat tgccgcggat ttcacgcccg
    50521 gcaatagcac ggctaacgcc gagttgctgt gcgataaatt tatcaagtcg catgtgtgtg
    50581 attttgcctg taaaaaacgg aggtcgggta atgacccgaa aatctgaact gttgtctgcc
    50641 cagtatagcg gtctaataac ttccctcaag ggaaaaagat ccgtggcata ctattagcag
    50701 aataatctac ctacgcgaga ccatgatttt tacactccgc ccatatcagc aagaagccgt
    50761 ggatgccacg ctcaaccatt ttcgtcgtca taaaacccct gccgtaatcg tgctgcccac
    50821 cggtgcaggt aaaagcctgg tgatagcgga actggcgcgg ctggcgcgtg gtcgcgtgct
    50881 ggtgctggca cacgttaaag aactggtggc gcaaaaccat gcaaaatatc aggcgctggg
    50941 gctggaagcc gatatttttg ccgccgggct aaagcgcaaa gagagccacg gtaaagtgat
    51001 atttggcagc gtgcagtcgg tcgcccgtaa tcttgatgcc tttcagggtg aattttcgct
    51061 gttgattgtc gatgaatgcc accgtattgg tgacgatgaa gagagccagt atcagcaaat
    51121 cctcactcac ctgaccaagg tgaatcccca cttacgcctg ctggggctga ctgccacgcc
    51181 ttttcgactg ggcaaaggct ggatctacca gttccattat cacggcatgg tacgcggcga
    51241 tgagaaagcc cttttccgtg actgcattta tgagctgccg ctgcgttata tgattaaaca
    51301 cggctatctg acgccgccag aacggctgga tatgccagtc gtgcaatacg atttcagccg
    51361 cttgcaggca cagagtaacg gactgttcag cgaagccgat ctcaaccgcg agctgaaaaa
    51421 acagcaacgc attacgccac acatcatcag ccaaatcatg gagtttgctg caacgcgcaa
    51481 aggggtgatg attttcgccg cgacggtcga acacgcaaaa gagattgtgg gattactgcc
    51541 tgccgaagat gcagcactga ttactggcga cacacccggc tctgagcgcg atgtgttaat
    51601 tgatgatttt aaagcccagc gttttcgcta tctggtcaac gtcgcggtac tgaccaccgg
    51661 atttgacgcc ccgcacgtcg atcttatcgc cattctgcgc cctaccgaat cagtgagtct
    51721 ttaccaacaa attgtcgggc gcggtctgcg cctggccccc ggcaagactg attgcttaat
    51781 tcttgattat gcgggtaacc ctcacgatct ctacgcgccg gaagttggta caccgaaagg
    51841 caaaagtgac aacgttccgg tacaggtttt ctgccctgcc tgcggttttg ccaacacctt
    51901 ttgggggaaa acgaccgccg acgggacatt gattgaacac tttggtcgtc gttgtcaggg
    51961 atggtttgaa gatgacgacg gtcatcgcga acaatgtgac ttccgtttcc gttttaaaaa
    52021 ttgcccgcaa tgtaacgcgg aaaacgatat tgccgcccgc cgctgccgcg aatgtgacac
    52081 cgtactggtt gatccggatg atatgttaaa agcggcgcta cgactgaaag acgcgctggt
    52141 attacgctgt agcggcatgt ctttgcagca tgggcacgac gagaaaggcg aatggttgaa
    52201 aatcacctat tacgatgaag acggcgcgga tgtgagtgag cgtttccgtc tgcaaacgcc
    52261 cgcccagcgt accgccttcg agcagctttt tatccgcccg catacgcgca caccgggcat
    52321 ccctctgcgc tggatcaccg ccgccgatat cctcgcccag caagcattac tgcgacaccc
    52381 ggattttgtc gtcgcccgca tgaaaggtca gtactggcaa gtgcgtgaaa aagtgttcga
    52441 ttacgaaggt cgttttcgtc gggcgcacga attacgcggt taatggtagt tttgattgat
    52501 gtataaggcg attgagtata gaatctcgcc cgcttttgca tacgcaaagc agatcactta
    52561 cctgttgctg ggtcgcctgt agcaggatta atttaagaga gaaagaaatg tttactatca
    52621 acgcagaagt acgtaaagag cagggtaagg gtgcgagccg ccgcctgcgt gccgctaaca
    52681 agttcccggc aatcatctac ggtggcaaag aagcgccgct ggctgtcgaa ctggatcacg
    52741 acaaagtcat gaacatgcaa gttaaagctg aattctacag cgaagttctg accatcgttg
    52801 ttgacggtaa agaaatcaaa gttaaagctc aggacgtaca gcgtcacccg tacaaaccga
    52861 agctgctaca catcgacttc gttcgcgctt aattgctgaa taagttgtaa aaaaccccgc
    52921 tccggcgggg ttttttgtct ctgcaaatta tgcctgatta cggtattgct attttttgca
    52981 ggccagataa gacgttcacg ccgcatctgg cattcagaac acaaaaccga ttaattgccg
    53041 ccagacgtcc ggcgctgcaa ttgatcgcgc aaattcggcg gcgtgccttt aatggtcagg
    53101 gtatcggttg ctggatccca gaagatgcgt tcacctaaca gcatcgcatc aaagttaata
    53161 gtcaagccac cgccgctacc ggcaaacttc gtcaactgac gcagtgtgct acggtctgcc
    53221 gggaagctct cttccagttc gtagcctttt tcagccgcaa actccgtgaa gctcacttcg
    53281 ctgacgcctg ccagctcttt cgacagcgat tccagttcaa tctcttcacc tgcctgcagc
    53341 tgctcattac agtagctata aacctgctgg cgcacattct gtcgctctgc tttatccagt
    53401 tgtgcttcgg cagtgaaatc atcgaccgct tgtaacagcc cacggttttg cgctttcgcg
    53461 ttcaaccctt cgctggcacc gaggaaatcc ataaagaaat ctgccacttt gcgccctact
    53521 cgccctttca ggaaagtgag atagcgggtc gactctggat tggtttccca ttcggttaaa
    53581 tcgatacgcg caacaatatc cgcatgattg atatcaagat aatgagttgg gttgatgtcg
    53641 agattttcgt taacgcgcat actgctcaga ttgctcagaa ctgccaccag caaatactcc
    53701 accgccagat agcgatagtg gcaaaacagc acgaatccac cgtcagcaaa aggatatttc
    53761 gctaattcgt cacgcaggcg accggttgcc gcgcggctaa atgccagaaa gtcctcttcg
    53821 ccctgacgct gtaagcgcag cgtctgcgcc agttcactct cttcgctaaa caagccgtaa
    53881 gctttatttt tggcgctata gacccgatgc agttctgcca ccatctcaac gacggtttcc
    53941 gtcggttcca gcaatgaatc gcgcaacacc agctcaaggt tctgctcatc acgcttgata
    54001 agctggtgca gagcaatctg gttgatatcc agactcatga taaactctcc tttaagaccg
    54061 ggcggtattc aaccaccgcc ggttaagcga cgcaataaaa gatggcggtc attttacaga
    54121 aggcagcgta acgagaatga tttaacgcat aaaaaagcag aaaaaaaacc gttgctacgg
    54181 taatatgttg ccctttcatc aactaactga tttcgattta tgccacaaat ttcccgctac
    54241 agtgatgaac aggttgaaca attgctcgca gagctgctca acgtactgga aaaacataag
    54301 gctccgaccg acctttccct gatggtgtta ggcaacatgg tcactaacct tatcaacacc
    54361 agcattgccc cggcccaacg ccaggcaatt gccaactctt ttgcccgcgc cttacagtcc
    54421 tctatcaacg aagacaaagc gcactaaggg aaacagataa caggttatgg taactcatcg
    54481 tcagcgctac cgtgaaaaag tctcccagat ggtcagttgg gggcactggt ttgcactgtt
    54541 caatattctg ctttcgctcg tcattggcag ccgttacctg tttatcgccg actggccgac
    54601 aacgcttgct ggtcgcattt attcctacgt aagcattatc ggtcatttca gcttcctggt
    54661 gttcgccacc tacttgctga tcctcttccc gctaaccttt atcgtcggct cccagaggct
    54721 gatgaggttt ttgtccgtca ttctggcaac ggcgggaatg acgctattac tgatcgacag
    54781 cgaagtcttt actcgtttcc atctccatct taatcccatc gtctggcagc tggttatcaa
    54841 cccagacgaa aatgagatgg cgcgcgactg gcagctaatg ttcatcagtg tgccggttat
    54901 tttattgctt gaactggtgt ttgcgacgtg gagctggcaa aagctgcgca gcctgacgcg
    54961 tcgtcggcgc ttcgcgcgcc cgctggccgc attcttattt atcgccttta tcgcctcgca
    55021 tgtggtttat atctgggccg atgccaactt ctatcgcccg atcaccatgc agcgcgctaa
    55081 cctgccgctt tcgtacccga tgacggcacg acgttttctt gagaagcatg gcctgcttga
    55141 tgcgcaggag tatcaacgcc gtcttattga gcaaggcaat ccagacgccg tttccgttca
    55201 gtacccgtta agcgaactgc gctatcgcga tatgggcacc gggcagaatg tgctgttgat
    55261 tactgtcgat ggcctgaact actcacgctt cgagaagcag atgcctgcgc tggcaggttt
    55321 tgctgagcaa aatatttcgt tcacgcgtca tatgagctcc ggcaacacta cagacaacgg
    55381 catctttggc ctgttttatg gcatctcgcc aagctatatg gacggcattt tgtcgacccg
    55441 tacgcctgcg gcgttaatta ctgcgcttaa tcagcaaggc tatcagctgg ggttattctc
    55501 gtcagatggc tttaccagcc cgctgtatcg gcaggcattg ttgtcagatt tctcgatgcc
    55561 gagtgtacgc acccaatccg acgaacagac cgccacgcag tggatcaact ggctgggccg
    55621 ctacgcacaa gaagataacc gctggttctc gtgggtctct ttcaatggca caaacattga
    55681 cgacagcaat cagcaggcat ttgcacggaa atatagccgg gcagcaggca atgtcgatga
    55741 ccagatcaac cgcgtgctca atgcactgcg tgattctggc aaactggaca atacggtggt
    55801 gattatcact gccgggcggg gtattccgct gagcgaagag gaagaaacct ttgactggtc
    55861 ccacggtcat ctgcaggtgc cattagtgat tcactggcca ggcacgccgg cgcagcgtat
    55921 taatgcgctg actgatcata ccgatctgat gacgacgctg atgcaacgcc tgctacatgt
    55981 cagcacacct gccagcgaat attcgcaagg tcaggatttg ttcaaccctc aacgccgcca
    56041 ttactgggtt accgccgcgg ataacgatac gctggcaatt accaccccga aaaagacgct
    56101 ggtgctgaac aataacggta aataccgcac ttacaactta cgtggtgaaa gagtgaaaga
    56161 tgaaaaacca cagttaagtt tgttattgca agtactgaca gacgagaagc gttttatcgc
    56221 taactgatta attataaatc agttagcaaa atatcttact tgcaatcggt gtggaaaacg
    56281 gtagtattag cagccacgag tcggcacgta gcgcagcctg gtagcgcacc gtcatggggt
    56341 gtcgggggtc ggaggttcaa atcctctcgt gccgaccaaa aatcccaaga aaaccaacca
    56401 attgcggttg gtttttttat atctgcattt aattcgataa acagacagcg acacatcacg
    56461 gcctgtttat tttctgttat cagaacgtcc agactacacc cgcctgagtt gtgctgcttt
    56521 cttcacggga gaaacggctg tcgataccaa gcgttagccc cgtcaatgtt tgctcaaagc
    56581 cagcgcctgc atcagtggtc acgttgttgc ggctgttaat tgccgaactc cacatcgccg
    56641 tatcgtaatt aacgcctttt acgctaccaa gacgcccacg cacggtgtcc agttctgcat
    56701 caaataccag cggttgtgct gccgccatat tcagcacatc cgtggttgaa gggggcattt
    56761 gtgcgcgatt ctctgcgaga ctccagctat ggttgccatt atccagcaag gtatattcat
    56821 acgtactgat atcaacaacg cctccggcat tgcccaacgt aaatgccgca tcaccgcgcc
    56881 cgttgttacc agtgtaaggt tatctcctgc tgccgggctg gcaccggtgt ccgtcacgaa
    56941 tattttgaaa tcacctgttg ctttaacctc cagtagccct cccttttcta taacggcaaa
    57001 gacggcaatc ccatcttttt caacaattaa gcgccctcct ttaatcgtgg gatcagtcgc
    57061 cgcgccataa acctgtagca ctccattatt gatcgtagtc tggtttaccg cgccacccat
    57121 ttgaacaatt tgataaccat catttaatac tgagtattct gcggtgccat ttatttcaat
    57181 gtactgatat cccccgttaa tttcagtatt actacttaca ccaaattctt atatatgctg
    57241 ttctccgcca ggattgattt tcgtattatt ggcggcccta caaaccagct ggatatcttt
    57301 ttcccggaca acaccatcaa ctgtctcacc atactcaaca gttgatgcca atgctatggg
    57361 cgaatataac gctgtagcca ccagaacaga tattgcggaa cgacaaagag aaacagaacc
    57421 agattggtgc attaagcttt catcctatga aattaattgc tgttaaaagc attggggaca
    57481 gaaaataccc atagctccat acccggagtc agttttaaaa aactgtttaa agaaatgcac
    57541 aagtattgtg attgattttt tagttgtttt tcctgatgag aagctgatgc aaaattccgt
    57601 ctttataatg aagatgatgc caaagcgaac gacaaagttg tagttttcac tacatgtcca
    57661 tacataaaat ggggtaacat tcacgcgcct ggtagcgtta ccaacgctac gctcaaacat
    57721 aatgattcta ataaaacctc aggagactac tatgcctgaa gcaacgcctt ttcaggtgat
    57781 gattgtggat gatcatccac ttatgcgacg cggtgttcgt cagttactgg agcttgattc
    57841 tggctttgaa gtggtcgccg aagcgggcga cggcgcaagc gcgatcgatc tggcgaatag
    57901 actggatatc gacgtgatct tgctggatct caatatgaaa ggcatgagtg gcctggatac
    57961 cctcaatgcc ttgcgcaggg atggcgttac cgcgcaaatt attatcctga ccgtatcaga
    58021 tgcctccagc gatgtctttg cactgataga cgcaggcgca gacggttatc tgttgaaaga
    58081 cagcgacccg gaagtgttac tggaagcgat tcgtgccgga gcgaaaggca gcaaagtctt
    58141 tagcgaacgc gtcaatcagt acttacgtga acgtgaaatg tttggcgcgg aagaagaccc
    58201 cttcagcgtg ctgacagaac gcgagctgga tgttctgcac gagctggcac aggggctgtc
    58261 aaataaacag attgcctcgg tgttgaatat ttccgagcag acagtaaaag tccatattcg
    58321 caatctgctg cgtaaactca acgtccgctc acgcgtggcg gccaccattc tgttcctgca
    58381 acaacgcggg gcacaataaa aatagcccga tggcatcacc atcgggccac tttttattta
    58441 ctctcctgcg gcgacaaatg ttgcatcgcc tgcgcgatac tacgttcgat caccgcacgg
    58501 cgagtatcgt tggcaggtaa gagtttcaac atcatttccc acgcggcaac ggcttcgcca
    58561 aatcgttgct gctcaaaggc attaaatgca tacatgctta acacacggat attgctatgg
    58621 tccgttctca ccaactggcg cagcagttcg ccgccgaggc ggttgtcgtt gggatcagat
    58681 gaacgtgtca acgcttcagc gtatcccagt gcagcatcgc tgtttttcgg atcgaggcga
    58741 tacgcagtgg cgtatgcatc ggtggcgata ctggcgttac ccagcgccat gccaacgcgg
    58801 cccaacataa tccagccttc tatatctccc ggattttttt gcagttgagt acgcatcccc
    58861 agcgcaaggc gcgacatctc ttcttcgttg agcggatcgg ctttcggatc cagcgccctg
    58921 tccagtaacg ccggagcctg tgccgtggcc tgctgccaga ttttcacctg ctgataattg
    58981 ccagtctggt agtagctgac gccagccaca attaacgcca ccacaatacc cggcagataa
    59041 acaacatatc cggcacgctt accttccggc acgctttgtt ctggaaacgc ttccggcacc
    59101 acgcgtaccc gacgccgcga acgggcgtat atgacccaac cgccaatgcc aatagccact
    59161 actggcagca cccacagcag cacggtcagc ggcgttaacg gcggatcgta agtgacgaag
    59221 ttgccgtaac gcgccaccat ataatcgaca atctctttct tacttttgcc ttcctgcatc
    59281 agttcataca ctttctggcg caggtcggtg gcaatcatcg agttggaatc ggcaatgctg
    59341 ttgttctggc atttcgggca gcgcagttct tcagtgagct gacggaactg ttgttcctgt
    59401 gcttcatctt taaactgcaa cacgtcgatg gtcgccagcg ctgagccgga gatcatcagc
    59461 atcagcacgc ccaataaaaa cctcattgtg cggcctcctt actgtatttc tcccacagtg
    59521 gcttgatctc ttcttcccag acgcgaggat tgagatcgcc cgcatggcga tagcgaatga
    59581 tgccgttgcc gtcaataagg aacgtttctg gcgcgccata gacaccgaga tccagcccta
    59641 acatgccgtc gccatcaaac aggcttagcg cgtaaggatt gcccagctct ttcagccagc
    59701 tgattgcctt ctggcgatcg tctttatagt tcatgccgac cacgcggatg ccctgcgcag
    59761 aaagctgatt cagatattga tgttccgcac ggcaggtcgg acaccaggtc gcccagacgt
    59821 taagcagcac cggtttgccc tgagtcagca catccgcctg ataaaactgc cccggattgt
    59881 ccagtgattc gaggcgaaac ttcggcactg gcttgccaat gagcgccgat tccagattgg
    59941 tcggatcatc cccttcggca ttacgcgcca gctgccacag cagcgccgcg gcaatcgcca
    60001 ggaagataat caacggaatt aacaatacct tgcgcttcat gcagcctccg gcgcagtttt
    60061 ctgcgggttc acgcgcttac gatagcgagg atcaaacaga cacagcagtc cgcccaacgc
    60121 catcatcagc ccgcccgccc aaatccagcg aacaaatggt ttgtagtaaa gacgcacggc
    60181 ccacgcgccg ttttccagct cttcaccgag tgccgcgtac aggtcacgcg tgatgccacc
    60241 gtcaatcgcc gcttcggtca tcatcgaccc ggcagtgttg taataacgtt tttctgcata
    60301 cagcactgtt tccggcttgc catctcgcgt tacgccgata gtcgccacac cgccacgcca
    60361 gttcggaccg gtcacctctt tgacatcacg gaaggtgaag cgatattcat gaatatcgac
    60421 gctatcgccg gacttcatgc gcacatcacg ctcaacgcta tagttctgac taaaagcaat
    60481 gccaacaatt gtcactgcca gccccaggtg agccgccacc atcccccaat aactgaaggt
    60541 ggttttcgtg ccgcgtgaaa tacgcagcgc agcttccgca attgccagca ccgcaatcca
    60601 gcaggccatt gccaggccga gcactgtcat cgccacaact ttgctttcga acagccacgg
    60661 caacagcagc gacagcacca gcgtagagat gaaggcgata atcaacaaat tgcggatttt
    60721 acgtgggcga tcgcgtcccc agcgcaccag agggccgaca ccaagcagta gcgcaaacgg
    60781 caccatcaac caggtaaaca tggtgttgaa gaacggttcg ccaatcgaaa tactgcccag
    60841 tcccagttgc ttgtgcacca gcggcagcaa cgtccccagc aacaccacca gcatcgcggc
    60901 gaccagcaaa acgttgttcg ctaacagcaa agattcccgc gaccacagcg cattgtttac
    60961 gcgtgagcga actttgtgtc cacgcgcggc aaacagcagc agcgaaccgc caatcaccag
    61021 caccataaag gcgaggataa acataccgcg cgccggatca gacgcaaacg cgtgtaccga
    61081 taccagcacg ccggaacgca ccaggaaagt ccccagtaga cacaacgaga aggcactgat
    61141 tgccagcagt aatgtccacg ctttgaagct ggcgcgttgt tcagtgaccg ccagtgagtg
    61201 catcagcgca gtccccacca gccacggcat aaacgaggcg ttttctaccg gatcccagaa
    61261 ccaccagccg ccccagccga gttcgtaata ggcccatgcg gaaccgagca caatgccgag
    61321 cgtcaggaag atccacgcag ccagcgtcca cggacgagta aaacgcgcat aagtgctgtc
    61381 cagacgccca ctcagcaaag aagcaatggc aaaagcaaac gccaccgaga aacccacgta
    61441 ccccatataa agcagaggcg gatggaagat cagccccgga tcctgcaaca gtgggttaag
    61501 atcacgccct tcaatcggga aattcggcag cgtgcgtgag aacgggttag aggtaaagag
    61561 aatgaacagc aaaaagccga cactgaccat ccccattatc gccagtacgc gggcaacaat
    61621 atccagcgga atacgctgac taaaaatcgc taccgcaaag gtccagccgc tcatcagtag
    61681 cacccacagc aggagcgagc cttcatgcgc gccccaggta gccgccacgc gataccacac
    61741 cggaagctgg gtattggagt tgctggcaac ataggtgacg gtaaagtcgt tgaccacgaa
    61801 agcattgacc agcaccagaa atgcgccagc cacagacata aacagcagcc aggcaaacaa
    61861 gcgggaagac gccatcatgc gcgcatctcc gcgcgctacg ccccatagcg gatacacgga
    61921 cagcagcagc gcaattccta gcgccagaca cagcagcccg ttaccaattt ctggcatcat
    61981 gatgctgggt ccttataaac actcgccggg cgacggtggt tagcttccat cgctttctca
    62041 acttctggcg gcgtgtagtt ttcatcgtgt ttcgccagca cttctttcgc gaggatatga
    62101 ttgccttttt ccagttcgcc ctgcaccaca acgccctgcc cttcacggaa cagatccggc
    62161 aaaatgcctt cgtaagagac atctactgaa ccttcagcgt cgtaaatggt gaaagttact
    62221 tttagcgaat tgggatcgcg ctgcacactc cccggcatca ccatcccgcc aacgcgcaga
    62281 cgctgaccga cttccggcat ctgctgagtt tcacgcttgc cgtagagaat ctcccccggc
    62341 gtataaaaga gatcgatatt cgagcgcagc gcatatagca ccagaccgat agtcagcgcc
    62401 agccccgcca gcacggaaca ggcaatccac aagcggtttt tacggcgaat attcatgcag
    62461 cctcctgctg ttgcgcagca cgtaaacgcg cctcacgcgc ccgctgttgc gccacgccac
    62521 gcagaattgc gcgatgttgc atcaccgagt gcacgaccaa aaccaccagc ggaataacgg
    62581 tcatcaccac cgccagccag acaaaaaagg cgtaaccgcc cattgcgaaa aattcattcc
    62641 aggaagcaaa tgcaggggtc atttacggcc tctttttagt atcagttcac tcacccacgg
    62701 acggcgtttt tccatcagca aaatcaaatt acgcatccgc atcagcgtca gcgtggcaga
    62761 cagcagcagg aaaccaaaaa tcgaccagcg cagcggcgaa cgcatcgccg gatcaatact
    62821 ttgctgcatc cgcgttgatc cctgatgcag ggtgttccac cactccacgg agtaatgaat
    62881 aatcggcagg ttcaccacgc caatcagcac caggatacct gccgcacggc cagccagacg
    62941 acggtcgtcg aaggcgtgcc acagggcaat gacaccaaca tacagaaaca gcagcaccag
    63001 ttcagaagtc agacgtgcat cccataccca ccaggtgccc cacatcggtt ttccccatgc
    63061 agagccggta accagggcaa taaaggtaaa cacggcacca atgggggcca tcgccgccac
    63121 cgccaggttg gccattttca tctgccagac caggccaata aacgctgcca ccgccattga
    63181 tgcataaatg cccatcgacc agatcgccgc aggcacatgc aggtagataa tgcggtagct
    63241 atttccctgc tgataatcag ccggagcaaa gccgaacccc cagatccagc cgacggtaag
    63301 cacgaccaca ctggcaattg ccagccacgg tataaaccag ccacagattt gatacagccg
    63361 tggtgggatc gccagttgat gcagtgtttt ccacatagtt tcgataccag actcgaacaa
    63421 aaatcagtaa tccagcgtta ttgaatgctg attcgtaacg ctgccgccgt cgcaaaagga
    63481 cttaatgtcg cggtgcctgc cagcaacgcg cctaaaattg ccagataccc atcaacgggc
    63541 aaatgcatag aagccgcgtc catcgcggcg gtggcaaaga tgagtaatgg gatagtcagc
    63601 ggtaacacca gtatgctgag cagcacacca ccgcgcttaa gtcccactgt cagcgccacg
    63661 cccggcgcac caagaaagcc aagcgtaggg gttcccagca gtagcgttaa cgccatcact
    63721 tgccagccat aaacatccat tcccagtagc attgctacca ggggcgaaag gatgagtaac
    63781 ggcagaccgg ttaccatcca gtgcgccatc accttcgcca gcacagcggc gggcaagggt
    63841 aacggcaaca acatcaattg ttcaagactg ccgtcctgca aatcgtcacg gaacagtcgc
    63901 tcgagtgcca gcaaggatga aagcagcgcg gcaacccaga taatgcccgg cgcaatacgc
    63961 gccagcagtt gcggctccgg accgatactg agcggaaaaa gggtaattac aatcaggaag
    64021 aaccacagcg ggttggcgat ttcggcgcta tggcgaaacg ctacgcgcag ctcaagacgg
    64081 aaaatgcgcc agaacatcat gcggcccccg tttgcgtcag tgaaatgcgg cgaattttac
    64141 tttcagcaac gttgagcggc tggtgggtag tcagaatcac aatccccccc tgctccgtat
    64201 gctgcgccat acgctgggtc agacgatcga cgccgttaac gtcaatcgcg gtaaaaggct
    64261 cgtcgagtat ccataacgtg gcacgggtca accacagacg cgctaaagcg acgcggcgtt
    64321 gttgcccggc agaaagttga tttaccggaa tatcttcgaa tccggcaagc ccggcctgcg
    64381 ccagggcttc cagacattgt gcggtatcgc catcgcgatg ataaaagtgc agattttcta
    64441 acgccgtcag ccgggttttg atccccggct gatggcctat ccataacagg ttttgatggt
    64501 agctgtcgcg tacctgatgc aagggctgcc cttgccagag aacctcgcct gcatcagggc
    64561 gagacaaccc cgtcagcaaa cggagaagcg ttgtcttccc cgcgccgttg ctaccggtga
    64621 tttgtaccca ctctcctgcg ttcagcgtaa atgataaacc actaaataag gttcgttcat
    64681 cccgctcgca aagtaactct ctggcttcaa gcatacccac gcaataaccc tgttaaaaac
    64741 ctggctcgac ttcacgcatg tcgggcagct tgtgcgctat ccctttatgg caatcaatac
    64801 aggtttgccc atctttcacc gcctggtcat gcatcttcgc ggcaaccgat ttctgggcgg
    64861 ttgtatccat atactcgaag ttgtgacagt tacggcactc ctgcgagttg ttgtccttca
    64921 tgcgccgcca ctcattctgt gccatcgtca ggcgatgagc ttcaaatttc tgcggcgtgt
    64981 caataacgcc aaaaatttta ccatacagct ctttacttgc tttgagcttg cgtatcatct
    65041 ttggcacaaa ctcgtgcgga acgtgacaat ccggacaggt cgcacggacg ccgctacggt
    65101 tgttgtagtg cacggaatcc atgtattcct gatacaccgt gttacgcatt tcgtggcagc
    65161 taatgcagaa ctcttcggta ttggcttttt ccatcccggt gttaaagccg ccccagaaga
    65221 cgatgccgcc gacaaaaccg atcaacagca gcgtccctag cgccagacgg ctgggggtac
    65281 gccaccattt ccacaggcgc ttaatcagac caggcttacg gtcagaattt cccataataa
    65341 cctcttattt cccgtaacct tttgatgggg taaaggtatt ccccacgatt ggcgcggtat
    65401 cggcctgcgg tacgtgacat tgcagacaga aataacgacg cggagccact tccgcgccca
    65461 ctttgccgtc gctgtccata aagtgagtag gactaatgcg cggcgcgcca gtggtgcgat
    65521 aactttcgac accgtggcac tgcaagcagc ggttggtatt ggtcgttacc tgataacctt
    65581 caacgctatg cgggatcatt ggcggctgat tcacatagtt cagcggcatc cgatcctgct
    65641 cttttggcat ccgaatggcc ccttcctgcg tcccggatac ttccggcgac tggctaaaat
    65701 cgacgccatt agccgcccaa accgcgccgc ttaccaccag ggccagcatc gccgtccatt
    65761 gacacagcgc tttcttcagg tcatggcttt tcatgatttc gctcccgaac tccatcgtgt
    65821 agttattgta aatacatcct cagaacagac atccacgcag cgaccgcacg tcatgcaatc
    65881 gcggctggtg acctgcaccg ggctttgttc atccagcacc ggggcacgta gcacatgcgg
    65941 ttccgggcaa acatgaaaac aatccataca gcggttacat ttctgccgat cggtggccgc
    66001 aacggtaatc acacctttgc tacccagcac gccatacagt gcaccaaccg gacaaatgtg
    66061 cccgcaccag ccgtgttcaa cgaccagtag atcaaataaa aacagcgcga gaataagcag
    66121 cgcgccgctg ccgaagccca ttaccaggct acgccccatc agagaaacgg ggttgatcca
    66181 ttcccagatg agcgtgccag ttaaggccga tcccaccaga atgaccacca acagcacgta
    66241 gcgaatatgg cgagggatcg tcgcagactg attgaggtca aatcgtctgc gtaaccaatt
    66301 tgctaagtcg gtaatcgggt tcagcggaca gacccagctg caaaataatc gctttcctgc
    66361 cagggcgtag agcacggtga taatcaccgc accggtcaac gccaccgtgg caggcagatg
    66421 accgctggca agactttgca gtgtcatcag cggatcggtc agtggaacgg tgtcgaataa
    66481 caggctactg ctgtagttgc cgtgcaagat ccacacacca aaccacggac cgctcaaaaa
    66541 catccccagc acgaagaact gacaaaggcg acgtaacact aaccaacggt gactgcgcca
    66601 ccagcctttt ttctccagcg cctcgcgccc ggcgtcacgt ttacgatttg ccattgttcc
    66661 cctccagcca gccgaagcgg taatggtgac ctaactcccc tttcgccagt gacagcggta
    66721 acaccttgat tgccggttgt tccagcacgc acactttttc gcacttaccg cagccggtac
    66781 aggcgtcgct gtgaaccgtc ggcagaaagc gggcgtgctt accggtacgc gtgttgcgct
    66841 ccagctccag ggtgatggcc acatcaattt tcgggcattc gcgataacaa acatcgcagc
    66901 gcagcccctg aaagttgaga cagttttcct ggtccaccag taccgccagc cccatccgcg
    66961 cgtcgtcgat cgattcgatc tcacgatcca gcgcaccgct cgggcacact ttggcgcacg
    67021 gaatgtcctc acacatttcg caaggaatat cccgtgcgac aaaatatggc gtgcccgccg
    67081 acagaccaga ggccagggtc gccagtttta aggtgtcgta agggcaagcc tgaacacact
    67141 gaccgcaacg cacacaggca ctggcaaagg cgttctcgtt tatggctccg ggcgggcgca
    67201 accgcacgcc agatgcgcgt gcggtttgct gttgtaaccc cagcgccaca cccacggtag
    67261 ccagcccgcc tgctgtgcga acgacatcgc gcagaaagcg gcggcgacca ttttgaggtt
    67321 tcgctgaccg ggacataatg gcttacacct tctccagttt gaccgcgcac ttcttgaagt
    67381 ccgtctcttt cgagagcgga tcggtcgcat ccagcgtcag tttgttaacc agctgtgcgg
    67441 cgtcgaagaa cggcatgtac accaggccct gcggtggacg gttacggccg cgcgtttcga
    67501 caatcgagat cacttcgcca cggcgagaaa ccactttcac tttgtcgcca cggcgcagat
    67561 cgcgcgcttt cgcatccaac gggtgaataa acaggaccgc ttccgggaag gcgcggtgca
    67621 gttccggcac acggcgagtc atactgccgg tgtgccagtg ctccaggacg cgtccggtag
    67681 agagccacag gtcgtactct tcatccggtg cttccgccgc cggttcgaac ggcagcgcga
    67741 agatcaccgc tttaccatcc ggtttaccgt agaacttata gccttcgccc gctttcacgt
    67801 acgggtcgtt accttcgctg taacgccact gcgtttcttt accgttcacc accggccagc
    67861 gcagaccgcg cgctttgtgg tagtcatcga acggtgccag atcgtgaccg tgaccgcgac
    67921 caaaccatgc gtactcttcg aacagccctt tttgcagata gaagcccagc tcgcgggatt
    67981 catcgttcag ctgatcttcc gccagttcgg ataccgggaa tttgctcact tccggggtgg
    68041 catacagaac ttcgtacagc gttttgccac gcagttccgg tttcttcgcc agcagctctt
    68101 ccggccatac ttcttcagtt ttaaagcggc gggagaactg gactaactgc cagagatccg
    68161 atttcgcttc gcccggtgcc tgtacctgct gacgccagaa ctgagtacgg cgttcggcgt
    68221 taccgtaagc gccctctttc tctacccaca ttgcggtcgg caggatcaag tcggcggcca
    68281 gcgcactgac tgtcggatac ggatcggaaa cgatgataaa gttgcgcgga tcgcgccagc
    68341 ccggcatacg ctcttcatta atgttcggtc cggcctgcat gttattggta cacatggtcc
    68401 agtaaacatt cagtttgccg tctttcagcg cacggtcttg tgccaccgcg tgcagaccga
    68461 ttttcgccgg aatggtgccg ctcgggatat tccacttctt ctcgcagata tcacggtgtt
    68521 tctcgttagt caccaccatg tccgcaggca gacggtgagc aaaggtgccc acttcacgcg
    68581 cagtaccaca cgcggaaggc tgcccggtca gggagaacgg accgcaaccc ggctgggaaa
    68641 ttttgccggt cagcaggtgc aggttgtaga ccaagttgtt ggcccacaca ccacgggtat
    68701 gctggttgaa gcccatcgtc cagtaggaga tgactttctt gttcggatcg gcatacagct
    68761 gcgccagttg ttctaactgg tctttcggca cgccggtcat ttcggcggtt ttttccagcg
    68821 tatattcagc aacgaaggct ttgtaatctt caaagctcat cggttcggag gcgtcagaac
    68881 ccggattctt cgctgctttt tccagcggat gggtcggacg taaaccgtag ccgatgtccg
    68941 tcgccccttt gcgcaggtta acgtgtttgc tgaagaagtc ctgatttatc gcattgtttt
    69001 gaatgatata gttggcgata tagttcagga tcaccaggtc agattgcggc gtaaagatga
    69061 tgccgttatc cgccagctcg aagctacgat gctggtaggt agaaagcacc gccacggtga
    69121 cgttctggtt agaaagacga cggttagtga tgcgcgacca gaggatcggg tgcatctccg
    69181 ccatgttgga gccccacagc acaaacgcgt cagcctgctc gatgtcgtca tagcagccca
    69241 tcggctcatc cataccaaag gtacgcataa agccaactac tgccgacgcc atacagtgac
    69301 gcgcgttcgg gtcgatgttg ttcgaacgga agcccgcttt gaacaacttg gacgcggcat
    69361 aaccttccca gatagtccac tgaccagaac cgaacatacc gatcgattcc ggcccttttt
    69421 ctttcagggc ggttttgaat ttatcttcca tcacatcgaa ggcctgatcc caggtgattg
    69481 gggtaaattc gccttctttg tcatatttac cgtttttcat acgcagcagc ggctgcgtca
    69541 aacggtcttt accgtacatg attttgggca ggaaatagcc cttaatgcag ttcaggccac
    69601 ggttaaccgg tgcgtccggg tcgccctgac aggccaccac acgcccctgc tgcgttccga
    69661 ccagaacgcc gcaacccgta ccgcagaaac ggcacggcgc tttatcccat ttaatggctt
    69721 cctgctgacc aacaacggcg cgggcaacgc ccggcacgct gagaccggca gccgccgcag
    69781 cggccgcaac ggcgttagct ttcataaagc tacgacgact gagtttcatg gtgtttcctc
    69841 accttgctct tcctgctggt gataaaccag cgacaccgcc agcacgccct ctacgttgcg
    69901 tactgactca atggtttgga tcagcgtttc gctgtcttct gcttccacca ccacaatcaa
    69961 ctgaccgctc ggcgcgtcgc tgacagcaac ttcacagccg gtaaaggcgt tcagttgggt
    70021 gctgatgtct gaaattcgtt cgcttttggc ctgcacgacc aggctgcaaa cttgccagtt
    70081 agtgtgcatg gagatactcc gcagttatgg ctgataccgg acagttggct gcacatgctc
    70141 cacatccgtt gcaaagttgg ctattaagtt gcggctggta gatcccggag agcgtcgggc
    70201 gaaagataat tgccattggc tcacagctat cctgacagcg gcggcattca actgactgat
    70261 acgccaggca cgcgtcccca atggtgaact gcaaatccca ggccctggtg tggcgcggag
    70321 aaaatagcga ttcggggcaa gcctgcgcgc aggcgtaaca gaagctgcac tcgttatttt
    70381 tgaaattaac gctcggatag ccacccgcgc cgcgttgcag aatgttgttt tcacaagcat
    70441 taatacaggc gtcgcagcgg gtgcaatggg tcagaaaatg agattcatca ccggaccagg
    70501 gcggacggat accgttactg gctttgcgcc agcgaccagt gagtatgccc cgacgggatg
    70561 catcaatctt cacattgacc ttccatcatt aacgcgctct gaaaattgag agcgaccaaa
    70621 taaaccgcat aattaataag ccatttttat agccgctaag atattaaagg atgtgtcaaa
    70681 gatgcatacc ccgatcgggg taaaacctgt agcaggatca aaagagtggc gggaagcgtg
    70741 gcaaaaacgg gcttttgctc acatttcaaa tggttataaa catatttata tagcgattaa
    70801 ttcgccagag atatttctgc tggtttgttt tctcatcaga atttaacact aaaagagcag
    70861 gtaaaattat ctgaatgttc tttaagttat tcataaagca aat
//
LOCUS       DAZFFE010000009        67943 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFFE010000009 DAZFFE010000000
VERSION     DAZFFE010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.009"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            84..1046
                     /gene="yfbL"
                     /locus_tag="ACHJ69_000748"
     CDS             84..1046
                     /gene="yfbL"
                     /locus_tag="ACHJ69_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFO5013802.1"
                     /translation="MNFAFIILFLFSLPLIIFYQPWVNALPPTPRHASPEQLEKTVRY
                     LTQTVHPRSADNIDNLNKSAEYIKEAFISSGARVTAQDVPITGGPYKNIVANYGPADG
                     PLIIIGAHYDSVSSYENDQLTYTPGADDNASGVAGLLELARLLQQQTPGIGVQLVAYA
                     SEEPPFFRSDEMGSAIHAASLERPVKLMIALEMIGYYDSTPGNQDYPSPAMSWLYPDR
                     GDFIAVVGRMQDINAVRQVKAALLSSRDLSVYSMNAPGFIPGIDFSDHLNYWQHDIPA
                     VMITDTAFYRNKQYHLPGDIADRLNYQKMAQVVDGVTTLLYNSK"
     gene            1149..1652
                     /gene="yfbM"
                     /locus_tag="ACHJ69_000749"
     CDS             1149..1652
                     /gene="yfbM"
                     /locus_tag="ACHJ69_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO5013803.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTKKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1719..3176)
                     /gene="nuoN"
                     /locus_tag="ACHJ69_000750"
     CDS             complement(1719..3176)
                     /gene="nuoN"
                     /locus_tag="ACHJ69_000750"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO5013804.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLVGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3183..4712)
                     /gene="nuoM"
                     /locus_tag="ACHJ69_000751"
     CDS             complement(3183..4712)
                     /gene="nuoM"
                     /locus_tag="ACHJ69_000751"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO5013805.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALVTMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSNG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4876..6717)
                     /gene="nuoL"
                     /locus_tag="ACHJ69_000752"
     CDS             complement(4876..6717)
                     /gene="nuoL"
                     /locus_tag="ACHJ69_000752"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO5013806.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLVDAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSSPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6714..7016)
                     /gene="nuoK"
                     /locus_tag="ACHJ69_000753"
     CDS             complement(6714..7016)
                     /gene="nuoK"
                     /locus_tag="ACHJ69_000753"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO5013807.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7013..7567)
                     /gene="nuoJ"
                     /locus_tag="ACHJ69_000754"
     CDS             complement(7013..7567)
                     /gene="nuoJ"
                     /locus_tag="ACHJ69_000754"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO5013808.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7579..8121)
                     /gene="nuoI"
                     /locus_tag="ACHJ69_000755"
     CDS             complement(7579..8121)
                     /gene="nuoI"
                     /locus_tag="ACHJ69_000755"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO5013809.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8136..9113)
                     /gene="nuoH"
                     /locus_tag="ACHJ69_000756"
     CDS             complement(8136..9113)
                     /gene="nuoH"
                     /locus_tag="ACHJ69_000756"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO5013810.1"
                     /translation="MSWISPELIEILLTVLKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRFGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9110..11836)
                     /gene="nuoG"
                     /locus_tag="ACHJ69_000757"
     CDS             complement(9110..11836)
                     /gene="nuoG"
                     /locus_tag="ACHJ69_000757"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO5013811.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFIILNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIIGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSQTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11889..13226)
                     /gene="nuoF"
                     /locus_tag="ACHJ69_000758"
     CDS             complement(11889..13226)
                     /gene="nuoF"
                     /locus_tag="ACHJ69_000758"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO5013812.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDSGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13223..13723)
                     /gene="nuoE"
                     /locus_tag="ACHJ69_000759"
     CDS             complement(13223..13723)
                     /gene="nuoE"
                     /locus_tag="ACHJ69_000759"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO5013813.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13726..15528)
                     /gene="nuoC"
                     /locus_tag="ACHJ69_000760"
     CDS             complement(13726..15528)
                     /gene="nuoC"
                     /locus_tag="ACHJ69_000760"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO5013814.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMVEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15622..16284)
                     /gene="nuoB"
                     /locus_tag="ACHJ69_000761"
     CDS             complement(15622..16284)
                     /gene="nuoB"
                     /locus_tag="ACHJ69_000761"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO5013815.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16300..16743)
                     /gene="nuoA"
                     /locus_tag="ACHJ69_000762"
     CDS             complement(16300..16743)
                     /gene="nuoA"
                     /locus_tag="ACHJ69_000762"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO5013816.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17373..18311)
                     /gene="lrhA"
                     /locus_tag="ACHJ69_000763"
     CDS             complement(17373..18311)
                     /gene="lrhA"
                     /locus_tag="ACHJ69_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO5013817.1"
                     /translation="MISANRLIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19231..20448
                     /gene="alaA"
                     /locus_tag="ACHJ69_000764"
     CDS             19231..20448
                     /gene="alaA"
                     /locus_tag="ACHJ69_000764"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO5013818.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPCTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20532..21131
                     /gene="yfbR"
                     /locus_tag="ACHJ69_000765"
     CDS             20532..21131
                     /gene="yfbR"
                     /locus_tag="ACHJ69_000765"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO5013819.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELQDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21190..23022)
                     /gene="yfbS"
                     /locus_tag="ACHJ69_000766"
     CDS             complement(21190..23022)
                     /gene="yfbS"
                     /locus_tag="ACHJ69_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO5013820.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALVVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPI
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23109..23759)
                     /gene="hxpA"
                     /locus_tag="ACHJ69_000767"
     CDS             complement(23109..23759)
                     /gene="hxpA"
                     /locus_tag="ACHJ69_000767"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO5013821.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLSPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23770..24264)
                     /gene="yfbU"
                     /locus_tag="ACHJ69_000768"
     CDS             complement(23770..24264)
                     /gene="yfbU"
                     /locus_tag="ACHJ69_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO5013822.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24347..24802)
                     /gene="yfbV"
                     /locus_tag="ACHJ69_000769"
     CDS             complement(24347..24802)
                     /gene="yfbV"
                     /locus_tag="ACHJ69_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO5013823.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25140..26342
                     /gene="ackA"
                     /locus_tag="ACHJ69_000770"
     CDS             25140..26342
                     /gene="ackA"
                     /locus_tag="ACHJ69_000770"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO5013824.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26417..28561
                     /gene="pta"
                     /locus_tag="ACHJ69_000771"
     CDS             26417..28561
                     /gene="pta"
                     /locus_tag="ACHJ69_000771"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO5013825.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIIANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSTKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRENYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28751..30271
                     /gene="yfcC"
                     /locus_tag="ACHJ69_000772"
     CDS             28751..30271
                     /gene="yfcC"
                     /locus_tag="ACHJ69_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO5013826.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAIFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30305..30847)
                     /gene="yfcD"
                     /locus_tag="ACHJ69_000773"
     CDS             complement(30305..30847)
                     /gene="yfcD"
                     /locus_tag="ACHJ69_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO5013827.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30905..31456)
                     /gene="yfcE"
                     /locus_tag="ACHJ69_000774"
     CDS             complement(30905..31456)
                     /gene="yfcE"
                     /locus_tag="ACHJ69_000774"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO5013828.1"
                     /translation="MKLMFASDIHGSLPATERVLELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31512..32156)
                     /gene="yfcF"
                     /locus_tag="ACHJ69_000775"
     CDS             complement(31512..32156)
                     /gene="yfcF"
                     /locus_tag="ACHJ69_000775"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO5013829.1"
                     /translation="MSKTAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPAWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32292..32939
                     /gene="yfcG"
                     /locus_tag="ACHJ69_000776"
     CDS             32292..32939
                     /gene="yfcG"
                     /locus_tag="ACHJ69_000776"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO5013830.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAVTLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQHGDERSDS"
     gene            32996..33358
                     /gene="folX"
                     /locus_tag="ACHJ69_000777"
     CDS             32996..33358
                     /gene="folX"
                     /locus_tag="ACHJ69_000777"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO5013831.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33379..34272
                     /gene="yfcH"
                     /locus_tag="ACHJ69_000778"
     CDS             33379..34272
                     /gene="yfcH"
                     /locus_tag="ACHJ69_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO5013832.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLDLGHQITVVTRNPQKASSVLGPR
                     VTLWQGLAEQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKKL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34320..35210)
                     /gene="rpnB"
                     /locus_tag="ACHJ69_000779"
     CDS             complement(34320..35210)
                     /gene="rpnB"
                     /locus_tag="ACHJ69_000779"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO5013833.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGAGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDARRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMMEKGFD
                     HEVILTLTRLSPDDLIAQSH"
     gene            complement(35406..36179)
                     /gene="hisP"
                     /locus_tag="ACHJ69_000780"
     CDS             complement(35406..36179)
                     /gene="hisP"
                     /locus_tag="ACHJ69_000780"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO5013834.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36187..36903)
                     /gene="hisM"
                     /locus_tag="ACHJ69_000781"
     CDS             complement(36187..36903)
                     /gene="hisM"
                     /locus_tag="ACHJ69_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5013835.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36900..37586)
                     /gene="hisQ"
                     /locus_tag="ACHJ69_000782"
     CDS             complement(36900..37586)
                     /gene="hisQ"
                     /locus_tag="ACHJ69_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO5013836.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTREQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37676..38458)
                     /gene="hisJ"
                     /locus_tag="ACHJ69_000783"
     CDS             complement(37676..38458)
                     /gene="hisJ"
                     /locus_tag="ACHJ69_000783"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO5013837.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRIGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38679..39461)
                     /gene="argT"
                     /locus_tag="ACHJ69_000784"
     CDS             complement(38679..39461)
                     /gene="argT"
                     /locus_tag="ACHJ69_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO5013838.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGPSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39727..40296)
                     /gene="ubiX"
                     /locus_tag="ACHJ69_000785"
     CDS             complement(39727..40296)
                     /gene="ubiX"
                     /locus_tag="ACHJ69_000785"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO5013839.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAVTLPEDLFARWQGA"
     gene            complement(40391..41908)
                     /gene="purF"
                     /locus_tag="ACHJ69_000786"
     CDS             complement(40391..41908)
                     /gene="purF"
                     /locus_tag="ACHJ69_000786"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO5013840.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41945..42433)
                     /gene="cvpA"
                     /locus_tag="ACHJ69_000787"
     CDS             complement(41945..42433)
                     /gene="cvpA"
                     /locus_tag="ACHJ69_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO5013841.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42965..43627)
                     /gene="dedD"
                     /locus_tag="ACHJ69_000788"
     CDS             complement(42965..43627)
                     /gene="dedD"
                     /locus_tag="ACHJ69_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO5013842.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPLKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKEKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43617..44885)
                     /gene="folC"
                     /locus_tag="ACHJ69_000789"
     CDS             complement(43617..44885)
                     /gene="folC"
                     /locus_tag="ACHJ69_000789"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO5013843.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWTFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44955..45869)
                     /gene="accD"
                     /locus_tag="ACHJ69_000790"
     CDS             complement(44955..45869)
                     /gene="accD"
                     /locus_tag="ACHJ69_000790"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO5013844.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIIRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(46025..46684)
                     /gene="dedA"
                     /locus_tag="ACHJ69_000791"
     CDS             complement(46025..46684)
                     /gene="dedA"
                     /locus_tag="ACHJ69_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO5013845.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(46767..47579)
                     /gene="truA"
                     /locus_tag="ACHJ69_000792"
     CDS             complement(46767..47579)
                     /gene="truA"
                     /locus_tag="ACHJ69_000792"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO5013846.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47579..48592)
                     /gene="usg"
                     /locus_tag="ACHJ69_000793"
     CDS             complement(47579..48592)
                     /gene="usg"
                     /locus_tag="ACHJ69_000793"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO5013847.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATATWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGVMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGSPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48658..49794)
                     /gene="pdxB"
                     /locus_tag="ACHJ69_000794"
     CDS             complement(48658..49794)
                     /gene="pdxB"
                     /locus_tag="ACHJ69_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO5013848.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLSEGQKLSVVLDVWEGEPELNVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49893..50888
                     /gene="flk"
                     /locus_tag="ACHJ69_000795"
     CDS             49893..50888
                     /gene="flk"
                     /locus_tag="ACHJ69_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO5013849.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIEPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILILVWLVR"
     gene            complement(50885..52063)
                     /gene="yfcJ"
                     /locus_tag="ACHJ69_000796"
     CDS             complement(50885..52063)
                     /gene="yfcJ"
                     /locus_tag="ACHJ69_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5013850.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVPAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSH
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52328..53548)
                     /gene="fabB"
                     /locus_tag="ACHJ69_000797"
     CDS             complement(52328..53548)
                     /gene="fabB"
                     /locus_tag="ACHJ69_000797"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO5013851.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53707..55713
                     /gene="mnmC"
                     /locus_tag="ACHJ69_000798"
     CDS             53707..55713
                     /gene="mnmC"
                     /locus_tag="ACHJ69_000798"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO5013852.1"
                     /translation="MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLARADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGHVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFKAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFSMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSNKQEAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIHYQYQLQDLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSEEDQLQNRQRLIDCFPHAQWAKEVDVSGKEARCGV
                     RCATRDHLPMVGNVPDYDATLVEYASLAEKKDEAVSAPVYDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSEEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55834..56112)
                     /gene="yfcL"
                     /locus_tag="ACHJ69_000799"
     CDS             complement(55834..56112)
                     /gene="yfcL"
                     /locus_tag="ACHJ69_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO5013853.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWEHLFQQASQQ"
     gene            complement(56146..56694)
                     /gene="epmC"
                     /locus_tag="ACHJ69_000800"
     CDS             complement(56146..56694)
                     /gene="epmC"
                     /locus_tag="ACHJ69_000800"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO5013854.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56694..57503)
                     /gene="yfcA"
                     /locus_tag="ACHJ69_000801"
     CDS             complement(56694..57503)
                     /gene="yfcA"
                     /locus_tag="ACHJ69_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO5013855.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALVGGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMS"
     gene            complement(57503..58327)
                     /gene="mepA"
                     /locus_tag="ACHJ69_000802"
     CDS             complement(57503..58327)
                     /gene="mepA"
                     /locus_tag="ACHJ69_000802"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO5013856.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDISLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPALWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58331..59416)
                     /gene="aroC"
                     /locus_tag="ACHJ69_000803"
     CDS             complement(58331..59416)
                     /gene="aroC"
                     /locus_tag="ACHJ69_000803"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO5013857.1"
                     /translation="MTGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59451..60383)
                     /gene="prmB"
                     /locus_tag="ACHJ69_000804"
     CDS             complement(59451..60383)
                     /gene="prmB"
                     /locus_tag="ACHJ69_000804"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO5013858.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALTVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            60549..61100
                     /gene="smrB"
                     /locus_tag="ACHJ69_000805"
     CDS             60549..61100
                     /gene="smrB"
                     /locus_tag="ACHJ69_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO5013859.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVDEWLPPKLP"
     gene            complement(61420..62277)
                     /locus_tag="ACHJ69_000806"
     CDS             complement(61420..62277)
                     /locus_tag="ACHJ69_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO5013860.1"
                     /translation="MKKTNLLFRLLILMVFMLGSRAGMGQTFRNVYIYYATFGWNSQV
                     ELYLDVAEVSSVYYGTYYQSNYVMTLGNLPNRTWTGTGSSPAPTITVKDGTIVNNSFC
                     PGMEALEAKDSRWECLKLPLDITVDGDVGGCPWLVSIRATSQVVVGSVDKDIYYGPNA
                     TVSVCPTVSVTPYDVSWDENYINKTKTLSLQSTGGVIEKTLSTYLMKDGKLCDGSQMN
                     EEGAYCRWVAQMITFSSSGCDNANVTVAPNRHPITDKQLHDMVVRVDTSSRQPIDSTC
                     RFQYTLNEL"
     gene            complement(62279..62806)
                     /locus_tag="ACHJ69_000807"
     CDS             complement(62279..62806)
                     /locus_tag="ACHJ69_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5013861.1"
                     /translation="MNLKRISLCAAVLATVLLTPHVQAAGNLHFYGNLLSKSCTLVVQ
                     GETLAEVHFPTISRKDLMVTGQSARVPVVFQLKDCKGPAQYEVRVTLTGTEDSEQPGF
                     LALDVSSAAQGVGIGMEKNDGTPVPINNTSGATFVLSNGNNNLNFNAWLQAKSGREVT
                     MGEFTASLTATFEYI"
     gene            complement(62803..63282)
                     /locus_tag="ACHJ69_000808"
     CDS             complement(62803..63282)
                     /locus_tag="ACHJ69_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5013862.1"
                     /translation="MKRLAWCLIYGFAGLAQAAINDVTFHGTLVSPPACTISDGKTIE
                     VEFRNVIIDNINGDNFRQDVPYTITCDPDVRDDAWEMSLTWTGSQTPYDDSAIETDVS
                     GLGIELQQNGQPFKLGTPLKIDPSTPPTLQAVPVKANDAALSDGTFSAYATLQVDYQ"
     gene            complement(63279..63782)
                     /locus_tag="ACHJ69_000809"
     CDS             complement(63279..63782)
                     /locus_tag="ACHJ69_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5013863.1"
                     /translation="MKAFFLTGSAALLLACGVTVFPAMAGGKIVNLTLRVLVDGPPPC
                     SVKGSAVEFGNVIIKSIDGANYRQAVGYTLNCSNSVSDDLQMQLQATTTTINGETVLS
                     TGISGFGIRIQNAADHSLVAVGNSSWLPFNINTQPDFEAVPVKQSGVELMASEFNATM
                     TMVVDYQ"
     gene            complement(63799..64551)
                     /locus_tag="ACHJ69_000810"
     CDS             complement(63799..64551)
                     /locus_tag="ACHJ69_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5013864.1"
                     /translation="MSYKLSSPMLVSTALMAMLTTASLTAHASVTPDRTRLVFNESDK
                     SISVTLRNNNEKLPYLAQSWLEDEKGNKIISPLAVLPPVQRIDAMMNGQVKIQALPDI
                     HTLPSDRESLFYYNVREIPPKSGKANTLQIALQTRIKLFWRPKALEKIDMRKPWQFKV
                     TLTRSGQDYTVNNPTPYHVIISDASTQKKGLTAAGFKPLVMPPKTSQPLKAKMASAPV
                     LTYINDYGARMPLIFRCEGNTCKVDEDQSSKG"
     gene            complement(64571..67219)
                     /locus_tag="ACHJ69_000811"
     CDS             complement(64571..67219)
                     /locus_tag="ACHJ69_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO5013865.1"
                     /translation="MPNHSNFRLRGIACYIALAISGGSVNAWADDSIQFAPRFLELKG
                     DTKIDLGKFSKKGYVDAGKYNLRVFINKQPLSDEYDINWYVSENDPTKNYACLTPELV
                     AALGLKEGIAKSLQWTHNDECLKPGQLDGMEVENDLSQSALLLTVPQAYLEYTSSDWD
                     PPSRWDDGIPGLIADYSLNAQTRHQEQGGEDSHDISGNGTVGANLGAWRFRADWQSDY
                     QHTRSNDDDDDSSNSTTSKHWDWSRYYAWRALPSLKAKLSLGKDYLNSDIFDGFNYIG
                     SSVSTDDQMLPPNLRGYAPDVSGVAHSSAKVTISQMGRVLYETQVPAGPFRIQDIGDS
                     VSGTLHVRVEEQNGQVQEYDVTTASMPFLTRQGQVRYKVMMGRPEDWNHKTEGGFFSG
                     GEASWGVADGWSLYGGALADKHYQSAAMGVGRDLAQFGALAFDVTHSHVNLDHDSAYG
                     KGKLDGNSFRVSYAKDFDELNSRVTFAGYRFSEKNFMTMSEYLDANQSDMARTGNDKE
                     MYTITYNQNFAAAGVSIYLNYSHRTYWDRPEQTNYNLMFSHYFNMGSIRNMSISVTGY
                     RYEYDDNADKGMYLSMSIPWSDSSTVTYNGSYGSGSDSSQVGYFKRVDDATHYQVNVG
                     TSEQHGSVDGYLSHDGSLAKVDLSANYHEGEYRSAGIALQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGIANVPVESNGAPVYTNMFGKAVVADINNYYRNQAYIDLNNLPEDAEA
                     TQSVVQATLTEGAIGYRKFKVISGQKAMAVLRLRDGSYPPFGAEVKNDEQQQVGIVDD
                     EGNVYLAGVNAGEHMMVFWEGSAQCEIVLPKPLPADLFSGLLLPCEQKGTAAPDSSAP
                     EIKPVIQDQTRQVTPTEAPTSISATQ"
     gene            complement(67300..67866)
                     /locus_tag="ACHJ69_000812"
     CDS             complement(67300..67866)
                     /locus_tag="ACHJ69_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5013866.1"
                     /translation="MSKFAKTAIAAAMVMGAVVSSSAFAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGAAKLTGGGTTTPKDFQIRLQDCVFTTETNMETTFTGTNYAGP
                     ANTDNYALFNVDSGVAMNHVSLVIGDTHGKGYKVGEKIVQPIVMDTSTSKGKDKQTLN
                     FKAWLVGETDAPDLGQFETLTTFQITYL"
BASE COUNT        16645 a        18525 c        17102 g        15671 t
ORIGIN      
        1 atcttaataa aatacaatct cgaaattcat aacaaataat gattcgacaa cgcaacccat
       61 taataatttg catatgaaaa aaaataaatt ttgcttttat tatattattt ctgttttcac
      121 tccctctgat tattttttac caaccctggg tgaatgcatt accgccaacg ccccgacacg
      181 caagtcctga gcaattagaa aaaacagttc gttatcttac acaaactgtg catccacgta
      241 gtgccgacaa tattgataat ctgaataagt ccgcagagta tataaaagag gcctttatca
      301 gtagcggagc cagagttacc gcgcaggatg tccccattac gggtggcccc tacaaaaaca
      361 ttgttgctaa ttatggtcct gccgatggac cgctgattat tattggtgcg cactatgact
      421 ctgtcagtag ttatgaaaac gatcaattga cctatacgcc gggcgcggat gataacgcca
      481 gcggcgtggc aggattactc gaactggcac gtttgttaca gcagcagact ccagggattg
      541 gcgtccaact ggtcgcctat gcgtcggaag aaccgccctt ctttcgtagc gatgaaatgg
      601 ggagcgcgat acatgcagct tcgcttgagc gtccagtgaa attaatgata gcgctggaga
      661 tgattggtta ttacgactca acgcctggta accaggatta cccatccccg gcaatgtcct
      721 ggctttatcc cgatcgggga gactttattg ccgtggtcgg cagaatgcag gatatcaacg
      781 ccgttcgtca ggtaaaagcg gcattgttgt catcccggga tttatctgtt tattctatga
      841 atgccccagg ttttattccc ggtattgatt tctctgatca ccttaattat tggcaacacg
      901 atattcccgc cgtaatgatt actgacaccg ctttttatcg taataaacaa taccacttgc
      961 ccggtgatat cgcagacagg ttaaattatc agaaaatggc tcaggtagtg gatggagtta
     1021 caaccttgtt atacaacagt aaataataaa aatttctcga ggcggatcca gaaaaagcaa
     1081 catgcgcatt ttattttctt tcgtctgttt taagatgcga aacatgaaat tcaacggagg
     1141 aaaaataaat gggaatgatt ggctattttg cagaaattga ctcagaaaag attaaccaac
     1201 tgcttgagtc tactaaaaaa cctttgatgg ataatataca tgacacgctt tcaggattac
     1261 gccgactgga tattgataaa cgctgggatt ttttacactt tgggctgaca ggcacctctg
     1321 cttttgatcc cgcgaagaat gacccgctga gtcgcgccgt gcttggtgaa cacagcctag
     1381 aagatggcat agatggcttc ctgggattga cctggaatca ggagttagcc gcgacaatcg
     1441 accgactgga atcactggat cgaagtgagc tgcgtaaaca gttttccatt aagcgactca
     1501 atgaaatgga gatttatccg ggagtaacct tttcagaaga gttagagggg caactctttg
     1561 cttcgatcat gcttgatatg gaaaagctga ttagcgcgta tcgacgtatg ctgcgacaag
     1621 ggaatcatgc gctgacggtt attgttgggt gatggtgtca gggcaccaaa aaaaaccgcc
     1681 gaacatgtcg gcggctttct gacttacaaa ctgactgttt acatcagcgg cattgctaac
     1741 cgcacaatgc taatcagcgg ttgtggccat acacccagca ccagtaccaa cagtgcagag
     1801 atcagcacca cgataccgcc tgcgctgtac tgccagtttg atggtgcatc gcgacccggt
     1861 tgttccggag cgtgcaggta taagctcacc gccacgcgca ggtagtagta aaggccgatt
     1921 gccgaaccaa caaccacggc acccaccagc caccacaagt gtgcctggac accgaccgcc
     1981 agcacgtaga acttaccgat aaagcccagc gtcattggga taccagccag tgaaagcatc
     2041 atcaccgtca tcactgccgc gaggatcgga cgatgccaga acagaccgcg gtaagagaac
     2101 agggaatcag catccgggcc acgatacggg ctggacatca ggctgaccac gccgaacgcg
     2161 ccgaggctgc tgaacagata accgaccagg taaaccccta ccgcttccat cgacatctcg
     2221 ccggtttgca gcgcaatcag cgccaccagc agatagccga ggtgagagat agatgagtaa
     2281 ccgagcagac gtttgatatt ggtctggctc agcgccatca ggttaccgaa gatgatggag
     2341 gcaaaggcga taatcgccag cactacgcga atcgcttcac tgtcacccac cggtgcgtaa
     2401 aggaacaaac gcatcaccac gccgaagata gcgattttgc tcgccgtcgc caggaaagtg
     2461 gaaaccggcg caggcgcgcc ctggtatacg tctggcgtcc acaggtggaa cggcaccaga
     2521 gagagtttga aaccgaggcc aacaatcatc aggccaaaac ctgccagcaa cagcggctcg
     2581 ttgagcatac cgtcgccgag gtttttaccc agtgcgacaa acgacaggtc gccagactgc
     2641 gcatacacca gcgccatacc gaacagcagg aaagaagacg ctgcggcaga aaggatggtg
     2701 tatttgatac tggcttccag cgaacgtttc tggcggaaag cgtaaccgac caggccaaac
     2761 agcggcaaag agatcagttc gataccgagg aacagagacg ccagatggtt ggcattcgcc
     2821 agcaggatcc cgcccagcgc ggcaattaac accaacaggt agaactcgtc cttgttgtcg
     2881 ttatagcctt caagccacgg gtaggcgaaa gtacaggtgg cgaggctcgc caacaatacc
     2941 agcccggtgt aaagcatggc gaaaccatca acgcgcatca gcggcgtaac gtccatagcg
     3001 cccgcctggc cgacaaacca gagcgaaacc agcgccgcgt taagcccaat aaccgagagc
     3061 gtagcgttga ggaaatgatt gcgtcgccac gcaatggaga gcatcacaac caccaccgtc
     3121 aagccgacga tcagcaacgg tagcagtgcg atcaggtttt gtggagttat tgtcatggcg
     3181 atttacggcc ttgtagtagt aacggaatta acaaaccact gctggatatt gccaatcgcg
     3241 gagtgcgagg tatccagaat cggctgcgga tagaagccca gcagtaccag cagcaccacc
     3301 agcaacaaga tcataaacag ctcacgcagc gacatccctg gcagttcctg gctggcaatc
     3361 tggcttttcg ctttgccgaa gtaagcgcga tgcagcatcg ccagcgaata aacagatgca
     3421 aagaccagcc caaaggtaga gataacggta atcaccggga caacctggaa gctgccgaac
     3481 agaatcataa attcgccgac gaagttaccg gtgccaggca tcccaagcgt tgccaccgca
     3541 aagaacagcg acagtgctgg cagccatttc atcttgctcc acagaccgcc catcatgcgc
     3601 atgtcgcggg tatggatacg ttcataaagc tgaccacaaa gaataaacag acccgccgcc
     3661 gacaagccgt gcgcaatcat ctggattacc gcgccctggt aggccaactg gctgccggtg
     3721 tagatagcaa tcagcacgaa gcccatgtgg gaaaccgagg tgtaggcgat cagacgtttg
     3781 atatcggtct gggcgaaggc catccacgca ccatagaaga tgccgataac acccagccac
     3841 atagcgattg gcgcgaactc tgccgacgcg ttcgggaaca gcggtaggga gaaacgcagc
     3901 agaccgtaag cggcggtttt cagcaagatc cccgcgagat caacggaacc ggcggtcggt
     3961 gcctgggagt gcgcatccgg taaccagcca tgcagcggaa ccaccggcat tttcaccgcg
     4021 aaggcgatga agaagcccag catcaacaga tattccacgc cgttagacat cggcgtattc
     4081 agcagctctt catagttgaa ggtccagacg ccggtcgcat tgtagtgaac gaaaaccagc
     4141 gccaggatgg cgatcaacat caccagacca ctcgcctggg tgtaaatgaa gaacttggtt
     4201 gccgccgtga tacgcgtttt accgtcagag gctttatgcc cccacagtgc gatcaggaag
     4261 tacatcggca ccagcatcat ttcccagaag aagaagaaca ggaacatgtc gatggcaagg
     4321 aacacgccga taacgccgcc caggatccac atcaggttga ggtggaagaa gccctgatat
     4381 ttttcgatct ctttccacga acagagtacc gccagcacac cgagcagacc ggtcagcacg
     4441 accatcagca acgacagacc gtcaatggcg agatggatag agataccaaa acgcgggatc
     4501 cacggcatgt cgaattcaga ctgccactgc ggaattccgg cggattgcgt cagtgaataa
     4561 ccgccctgca accacagttg cagcgacagc gccagcgtca atcccatggt taccagcgcg
     4621 atccagcgcg gcaccttgac gccaaagcgt tcggtctgcc agcacaggaa gccgccgata
     4681 aagggaatta atattagcca gggtagtaac atggcgatct ttattccttg taaaagtccc
     4741 gtcaggaccg gattttcaac gaattctcac gacagaaaat tctcttcgga ctgggggtag
     4801 tgtcggatgc gccgcgaaga cggcttatcc gacctacgtt cttatatgcc caggggcaat
     4861 cccacaatcc ttaactcaac gcagtaccat caacagtgcc agcaccacga ccgcaccgat
     4921 gctcatggat gccacatacc agcgcagata accgttctcg cttaacagca gacctttacc
     4981 tgcaaagcgg gaaaggacag ccgggatgtt catcattgag ttcagcggat cgcgtttcag
     5041 caaccaggca atacccagga acggcttgac gaacactttg tcgtacagcc agtcaaagcc
     5101 ccaggcgttg taccaccagg tgcccagcag acggcccggc gaactgttgg cgatggaggt
     5161 caccagagta cgtttaccca gccacagcca ggctgccagc agaatgccga ccaccgcgac
     5221 cacgccagag gtaatttcca gggtcaacat gctgccgtgc gccagctccg tcgtttgcgg
     5281 aagcacgccc tgcagcggcg gtacaatcag tgcgccaacg aaggtggaaa ggatcagcag
     5341 cacaatcagc ggcaggctgt gagttacccc tttcacggcg tgagcgtgaa tttgttcttt
     5401 tccgtggaag acgatgaaaa tcatacggaa ggtgtagagc gaggtcataa acgcaccgac
     5461 cagaccagcc accatcagat tgatatgacc attcgccatc gcacccgcga ggatctcatc
     5521 cttactgaag aagcccgcag tgaccagcgg cagtgccgac agtgctgcgc cgcccaccag
     5581 gaagcagaga taaaccagcg gaatagattt acgcagaccg cccatcttga agatgttctg
     5641 ttcgtgatgg caggccagaa tgacggaacc ggatgccagg aacagcagcg ctttaaagaa
     5701 cgcgtgagtc atcaagtgga aaatcgccgc atcccatgcc tgcacgccaa gcgcgaggaa
     5761 catgtagcca atctggctca tggtagagta agcgagaaca cgtttgatgt cggtctgtac
     5821 cagcgcggca aaaccggcca gcagcagcgt aaccgccccg acaatgccca ccagatgcag
     5881 aacttccggc gtcatcagga acaggccgtg ggtacgggcg atcaggtaga cacccgcggt
     5941 taccatcgtt gcggcgtgga tcagcgcgga gacaggcgtc gggcccgcca tcgcgtcgac
     6001 aagccatgtc tgcaacggca actgcgcaga tttaccgacc gcaccgccca gcagcatcag
     6061 cgtcgcccac atcagcatgt tattgccgtc agcaaagtgc gctggtgcca gttccaccat
     6121 ttcgcggaag ttcagggtgc ccagttcgtt gtaaagaatg aacagcgcga aagcgaggaa
     6181 gacgtcaccc acacgggtca cgacgaacgc tttcattgcc gctgcgccat tcttcggatc
     6241 ggtgtaatag aaaccgatca gcagatagga gcacaggccc acgccttccc agccgaggta
     6301 catcagcagc aggttgtcgg caagcaccag aaccaccatg ctggcgatga acaggttggt
     6361 gtaagcgaag aagcgagagt agccctcttc accgcgcata taccaggaag cgtacatgtg
     6421 aataaggaaa cccacaccag tgaccactga gagcatggtc agcgacaggc cgtccagcac
     6481 cagattgaaa ccgatgttaa agtcgcctac cgacatccac gtccacagcg gctggctgta
     6541 tgcctgctca ccgttagcga agaagtcaac gccgataaag gcggttacca gcgccgccag
     6601 gcccacagag ccaacgccga cgatcgccga gacgttttca gaccaacgcc cacgggagaa
     6661 cgccagcagg acgaagccaa tcaatggcaa aataatggtt aaggcaagca tgttcatccg
     6721 cgcatctcac ttactgaatc aatgttcagg ttctggcgac gacggtgaag ttgcagcagc
     6781 agcgcaaggc cgatactcgc ttctgccgcc gcgaggctga tggcgagaat gtacatcacc
     6841 tgaccgtcgg tctggcccca gtagcttccg gcgaccacaa aggccagcgc ggaggcgtta
     6901 atcatgattt ccagaccaat cagcataaac agcagattgc gacggataac cagaccggtt
     6961 aagccaagaa cgaataagat tgccgcgagg atcagtccat gttgtaaggg gatcatgcgt
     7021 gctcctccgt ttttcttttc gcgctgtcgt ctttacgatt gctcagcact tcacccgcac
     7081 gctcttcacg accgacgtgg aaggccacaa ccagacccgc gagcagcagc atagaagcca
     7141 gttccaccgc cagtacgtaa ggcccgaaca gcgtaatacc cactgcttta gcactgattg
     7201 gcgtaccgtc gataccttga tcgttaacac cgagaatggc gtaaacaatc accaccagca
     7261 tgatggctga caaaattgcc ggaccaatcc acacctgcgg tttcagccac tggcgttcct
     7321 gttcgatttc tgaaccgccc aggttgagca tcatcaccac gaatacgaac agcaccataa
     7381 tggcacccgc atagacgata atttccagcg caccggcgaa gtaagcgccc agtgagaaga
     7441 acaccccaga gatcgccagc agcgaaataa tcaggtacag cagcgcgtgt accggattgg
     7501 tatgggtgat cactcgcaag gtcgcaagta tggctatcag gccacagata taaaaagcga
     7561 actccattgc ccctctcctt acggtaacag gctcttgacg tcgataggct tggcttcgtt
     7621 ctctgcttcg cccttatctt tgccgtcgat tgccatacct gccatccggt agaagttata
     7681 ttccgggtat ttgcccggac cggagatcag cagatcctct ttctcgtaaa ccagatcctg
     7741 gcgcttgtat tcccccattt cgaaatccgg ggttaactga atcgccgtgg tcggacaggc
     7801 ttcttcgcac agaccacaga aaatgcagcg tgagaagttg atgcggaaaa attccggata
     7861 ccagcgaccg tctttggttt ctgctttttg cagcgagata cagccgaccg ggcaggctac
     7921 cgcgcagagg ttacaggcta cgcaacgctc ttcgccgtcc ggatcgcggg tcagaacgat
     7981 acgaccacga taacggggcg gcagatagac cggctcttcc gggtacattc gcgtttcgcg
     8041 tttggcgaac gcgtgcaggc cgatcatcca gatactacga acctgggtgc cgaaacctac
     8101 taacaattct tttaaggtca tggtcttatt gccccttatt gcgcctgcca gagaatgaca
     8161 gccgccgtta ccagcaagtt gatcagcgtc agcggcaggc agattttcca gccgaaggac
     8221 attacctggt cataacgcgg acgcggtaac gacgcacgaa tcaaaatgaa catcatcata
     8281 aagaacgcgg ttttcagcgc gaaccagatg aatggcggta acaacgggcc ttgccagcca
     8341 ccgaagaaca gcgtcaccat cagtgcagag atggtcacaa tcccgatgta ttcgcccacg
     8401 aagaacagac cgaacttcat accggaatat tcaatgtggt aaccatccgc cagttcctgc
     8461 tcggcttccg gctggtcaaa cgggtgacgg tgacacaccg ccacgcccgc gatggcaaag
     8521 gtaataaaac caaagaattg cgggatgacg ttccacacat gcgcctggct gttgacgatg
     8581 tcggtcatgt tgaatgaacc ggcctgcgcc accacgccca tcagggaaag cccgaggaac
     8641 acttcgtagc tcagggtctg cgcagaagca cgcatcgcac ccagcagcga gtatttgttg
     8701 ttacttgacc agcccgcaaa cagcaccgcg taaaccgcca gacctgccat catcaggaag
     8761 aacaaaatcc cgatgttcag gtcggcaacc acccaacccg gactgactgg cacaattgca
     8821 aaggccagca gcagcgaggt aaaggcaatc atcggtgcca gggtaaagat gacgcgatcc
     8881 gagaatttcg ggatccagtc ttctttaaag aacattttga tcatgtccgc aaccagctgg
     8941 agcgaaccgc cccagccaac acggttaggt ccaaaacggt tctggaacag acccagcagg
     9001 cgacgttcgc caaagctcat gaatgcccca caggtgacta ccaccagcag gatcaccacc
     9061 gctttaagga cggtcagcag gatctcaatc agttccggtg atatccaact cattgttgtg
     9121 cctccttgag atcctcaaga tgcgcgccag ccagcaccgg agcaatgccg gacataccca
     9181 tcggcaagcc cacctgccct gctgtcagcc cttcggcgat ttcaaccggc agcgtgaccg
     9241 tgttgccatc gtaactaaag gagacgcgtg tacctgcgtt cacacccaac ttcgcggcat
     9301 ccgctgggtt gagtttgatg tacggctgcg gcatacggct ctggaagacc ggagcacgct
     9361 gtgacaattc atcgctgcca aacaggtggt agtacggcgc gatacgccat ttcccgtcct
     9421 gcggctggaa gcgtgccggt acgctggtga agtaatccag accgttttcg ctggtttcaa
     9481 acagacgcac gcccggatcg ccaaaacgca gtttgccgcc cacttcgtcc tggaatttgt
     9541 tccacgcctg cggggagttc cagcctggtg cccaggcaaa cggcacttgc gaacggtgcg
     9601 cggtcggctg gttgttacct tccatcgaga aggtgaacat ggtgtcaata tcctgcggtt
     9661 gacgcggctc atgaacgctg atattggcgc gcatggcggt acgaccgcta tagcgatgcg
     9721 gttcacgggc cagtttctgc ccacgaatac ggaatgtcgc atccggcgca gcatctttga
     9781 tacccgccag ttccgggatt ttcgccacaa cagcgtcaat cacatggtcg agctgtgtcc
     9841 agtccacttc acggctcagc agggtgctgt gcagcgagtg cagccagcgc cagctttcca
     9901 gcatgacagt ttggctgtcg taataagccg gatcgtaaac ctggaagaaa cgttgggcgc
     9961 ggccttcgtt gttgatcacc gtaccgtcgc tttcagcaaa gctggcggcg gaaagtacca
    10021 gatgagcgtt ttccataatc gctgtgcgtt gatggtcaac caccatcacc agcggcgctt
    10081 tagccagcgc agcattcacg cgggtagcgg aagcgtgacg atgcagatcg ttttccagca
    10141 ccaccaccgc gtcggcgcgt ccggtttcca gttcagttaa cgcttcttca agcgatccac
    10201 cgcctataat gcccagcccc atgctgttga cggaacgggc aatcatggtg ataccgacgt
    10261 cagcgccgcg acctttcagg gctttcgcca cgttagccgc agcctgaatc acttcagcgc
    10321 taccagcgtt cgtcccggag ataatcaacg gtttcttcgc accagccagt gcctgcacga
    10381 tgacgtcgat tttgctttgc agctcaggtt cgataccgtc aaccgctggc gcggagttat
    10441 ccagcgcatg ggcgatggca aaacctaaac gcgcctgatc ttcaaccggt gcgcggtaag
    10501 tccacgcagc gatatcatcc agacgggtgt catcaacgtt ggtaacaaac agtggatgct
    10561 tcgcacgttg accgatgttg aggattgccg caatctgcca gtcagccact ttctgtgctg
    10621 ccgccatttc gcgtgcttta ccttttaccg cctggcgcac cgccagcgca acgcgcgcgc
    10681 cggtctgggt aacgtcttca cccagcacca gtaccgcatc gtaagattcg atttcacgca
    10741 gagccggagt ataaatgccg ccttcacgca gtactttcag cgccagttgc agacgttcct
    10801 gctcaccgtg agcgataccg gtatagaagt tttcttcgcc caccagctca cgcagcgcaa
    10861 agttgctttc cacgctggca cgcggagaac caataccgat cactttcttc gactgacgca
    10921 gaatatctgc tgcaccctgc attgcctgtt cggcgttgag gataatgaaa tcatcgccac
    10981 gacgctgtac tggctgacgc ggacgatctt tcaggttgac gtaaccgtaa ccgaaacgac
    11041 cacggtcgca gaggaagtag tggtttaccg taccgttgta acggttttcg attcgacgca
    11101 gttcgccata acgctcgccg gggctgatgt tacagccgat ggaacattgc tggcagatgc
    11161 tcggcgcaaa ctgcatatcc catttacggt tgtaacgctc ggagtgcgtt ttgtcggtga
    11221 atacgccggt cgggcaaatt tcgaccaggt taccggaaaa ttcgctttcc agcgtgccgt
    11281 cttccgggcg accgaagtag acgttgtcgt gcgcaccgta aacgcccagg tccgtgccgt
    11341 cagcgtaatc tttgtagtaa cgcacgcagc ggtagcaggc gatgcagcgg ttcatttcgt
    11401 gagagatgaa cggccccaaa tcctgattac ggtgggtacg tttggtgaaa cggtagcgac
    11461 ggaagctgtg tccggtcatc acggtcatat cctgaagatg gcagttaccg ccttcttcac
    11521 atactggaca gtcgtgcggg tggttggtca ttaaccactc gaccacactt tcacggaact
    11581 gtttcgcttc ttcgtcgtca atggaaataa aggtgccatc ggaagccggt gtcatacagg
    11641 acatcaccag gcgaccacgc gtgtcttccg cgttttggta ttgcttcacc gcacactggc
    11701 ggcaagcacc gacgcttccc agcgccggat gccagcaaaa gtaaggaata tcaaggccca
    11761 gagacagaca agcttccagc aggttgtccg ctccgttgac ctcgtattct ttgccgtcta
    11821 catgaattgt agccattagc atgcttccag ttttctcagt cagagactga gcgttaatcg
    11881 aaattcggtt accagcgctc tttcagcagg ttcggctgaa tcccattaat caaatgggta
    11941 ttgctgaacg gctgtttgat tcccgcctca aattcttcgc ggaaatattt gatggcgctc
    12001 tgtaacggct ccactgcacc aggtgcgtgg gcacagaaag ttttacccgg gcctaagaat
    12061 cgacacagtt gctcaagtgt ttcgatatcg cccggctggc cttcgccacg ctccagcgca
    12121 cgcagaattt tcacgctcca cggcagaccg tcgcggcatg gcgtacacca gccgcaggac
    12181 tcacgggcga aaaactcttc caggttacgc accagcgaca ccatgttgat ctcatggtca
    12241 accgccatcg ccagcgccgt acccagacgg cttcccgctt taccgatact ttcgaattcc
    12301 atcggcaggt caaggtgcgc ttcggtcagg aagtcagtcc ccgctccgcc tggctgccag
    12361 gctttgaatt tcagaccgtc acgcatacca ccggcgtagt cttcgaggat ctcgcgtgcg
    12421 gtggtgccga acggcagttc ccacagaccc ggatttttca cccgaccaga gaagcccatc
    12481 agcttggtgc cagcatcttt acttttcgag atgttctgat accactccac gccgttagcg
    12541 aggatcgccg gaacgttaca cagggtttcg acgttgttaa cgcaggttgg tttaccccat
    12601 gcaccggagg ttgccgggaa gggcggtttc gagcgtgggt tagcgcgacg cccttccagg
    12661 gagttgatta acgctgtttc ttcaccgcag atgtagcgcc ctgccccggt atggacgaac
    12721 agttcgaaat cgaaacctgt tcccataatg tttttgccaa gcagacccgc ttcggtggct
    12781 tcggcaatgg cacgacgcag attcactgcc gcttcgatat attcgccgcg caggaagata
    12841 tagccacggt aagctttcag cgcaaacgcg gagatgagca tgccttccac cagcaggtgc
    12901 ggcagttgct ccatcagcag acggtcttta taggtgcccg gctccatttc atcggcgtta
    12961 cacagtaggt aacggatatt catggattcg tctttcggca tcaggctcca cttcaggcca
    13021 gtcgagaagc ctgcgccacc gcgccctttc agaccagagt cttttacctg attaacgatt
    13081 tcgtccggag acaacccggt cagcgcctta cgcgcgcctt cgtaaccgtt tttgctgcga
    13141 tattcgtcca gccacactgg ctgtttgtca tcgcgcagac gccaggtcag cggatgcgtt
    13201 tcgggagtac ggataatgtt tttcatttat accgctccag cagttcaggg atcgcttccg
    13261 gggtcagatg cgcgtgagtg tcctcatcga tcatcatgtt tggcccttta tcacagttcc
    13321 ccaggcagca agttggcagc agcgtgaagc gaccgtcaaa tgtcgtttgc cctggtttga
    13381 tgttcagttt tttctccagc gccgcctgaa taccctggta accgttgatg tgacagacca
    13441 cgctgtcgca ataacggatc acatggcggc ctactggctg acggaagatc tggctataaa
    13501 acgttgccac gccttcgacg tcgctcgccg gaatacccag cacatcggcg atcgcgtgga
    13561 tcgcgccatc cggcacccag ccacgctgct tctgaacgat tttcagcgct tcaatggacg
    13621 ccgcacgcgg gtcttcgtag tggtgcatct cgtgctcaat cgcttcacgc tctgccgcac
    13681 tcagctcaaa agcctcggtt tgtggttgtt gattctcgtg cataattagc ggtccacatc
    13741 tgacataaca aaatcgatac tgcccagata aacaatcagg tcagacacca ggctgccgcg
    13801 gatcgccgcc ggaatttgct gcaaatgcgc ataactcggc gtacggatac gggtgcgata
    13861 actcatggtg ctgccgtcgc tggtcaggta gtaactgttg atccccttgg tcgcctcaac
    13921 catctggaag gattcattgg caggcatcac cggaccccac gacacttgca ggaagtgggt
    13981 gatcagggtt tcgatatgtt gcagcgtgcg ctctttcggc ggcggcgtgg tcagcgggtg
    14041 atccgctttg aacgggcctt ccggcatgtt gttgaggcac tgctcaagaa tacgcagact
    14101 ctggcgcaac tcttctactt tcagcattac gcgggtgtag cagtcagaaa caccaccgcc
    14161 caccggaatt tcgaagtcga agttttcata gccagaataa ggacgcgcct tacgcacgtc
    14221 gaagtcgatc ccggtggcac gcaggcccgc gccggtggtg ccccactcca gcgcctcttt
    14281 cgcgccgtaa gcggcaacgc cctgggaacg acctttcaga atggtgtttt gcagcgccgc
    14341 tttctcgtaa gacgccagac gtttcggcat ccagtcgagg aactcacgca gcaggcgatc
    14401 ccagccgcgc ggcaggtcgt gcgctacgcc gccaatacgg aaccacgccg ggtgcatacg
    14461 aaaaccagtg attgcttcca ccagatcgta aattttctga cgatcggtaa aggcgaagaa
    14521 cactggcgtc atcgcgccga cgtcctgaat aaaggtggag atatacagca ggtgactgtt
    14581 gatgcggaac agttcggaga gcattacgcg aatgacgtta acgcgatccg gcacggtgat
    14641 cccagccagt ttctctaccg ccagcacata aggcatttcg ttaacgcagc cgccgaggta
    14701 ttcgatacgg tcggtatacg gaatgtagct gtgccaggac tggcgttcgc ccattttctc
    14761 tgcgccacgg tggtggtaac cgatgtctgg tacgcagtcg acaatctctt cgccatcaag
    14821 ctgcaaaacg atacggaaag caccgtgcgc agacgggtgg ttcggaccga ggttgaggaa
    14881 catgaagtcc tcgttttcgg tgccgcgctt catcccccac tcttccggtt tgaaggtcag
    14941 ggcctccatc tccagatcct gtttggcttt ggtcagctca aacggcgaga attcggtagc
    15001 gcgcgccggg taatctttac gcagcgggtg acctttccag gtttgcggca tcatgatgcg
    15061 gcgcaggttc gggtgaccgt cgaatgtaat gccaaataga tcccaggttt cacgctcata
    15121 ccagttagcg ttcgggaata atttggtaaa ggtcggtacg tgcaggtcgt tttctgccag
    15181 cgccaccttc agcatgatgt cgcggttacg atcgatagaa atcagatggt agaaaacgga
    15241 aaaatccgcg gcaggtaacc cttcgcggtg tgtgcgcaga cgttcgtcca tgccgtgtaa
    15301 gtcaaacagc atgacgtaag gtttcggcag tttctttaag aaatcgccca cttccagtaa
    15361 ttgttcacgc ttgatccaca caacgggaac cccggtgcga gtcgcctgaa cagtaaaggc
    15421 atccggccca aaacggttgc gcagttcgcc aatcaccgga tcatcaagat gatcgcgggt
    15481 ctgccaggcg ggttcttgcg cggttaagtc ggtcatattg ttcaccattg caaatggtcc
    15541 gtggtgactg tcggcgcagt aagcttcgcg aaatagattt aggaataagc gaagaaaatt
    15601 ccctttgccg acaggcgcaa attaaatctc gtcaggtgta cgcaggttgg taacggcaat
    15661 gcgttcaccg cgcttgcgtt cgcgctctga ttgcatattg gcgcgataaa cgccctgatc
    15721 gccaaccacc caggagagcg gacgacgttc tttaccgata gattcctgca acagcatcag
    15781 tgcctgcatg tacgcttcag gacgcggcgg acagcccggg atatacacat caaccgggat
    15841 gaatttatca acgccctgca caacggaata aatatcgtac ataccgccgg agttggcaca
    15901 ggcacccatt gagataaccc attttggttc cagcatctgg tcatacaaac gctgaataac
    15961 cggtgccatt ttggtaaagc aggttcctgc aaccaccatc aggtcagcct gacgcggcga
    16021 agcacgcaat acttcagcgc caaaacgcgc cacgtcatgc accgcggtaa acgaagtcac
    16081 catctcaacg taacagcagg aaagaccgaa gttatacggc caaattgagt ttttacgacc
    16141 ccagttaacc atgtcattga gcttgcccat aaacacgttt ttgttaactt cttgctccag
    16201 agggtcggtt acgatctcct gcttttgcag ggggtaacgg tcattctcac cgttgggatc
    16261 tatgcgggtg agcgtataat ccatcttaat gcctcgcggt tagcgttgac gattagcgat
    16321 actgttcgtt tccgggttca tacgctcgcg gcgtgaacgc gcgggcgtcc agtccagcgc
    16381 gccaatacgc accagataaa ccagacctgc cagtaacaca aaaataaaaa ttgcagcttc
    16441 cacaaagcct acccagccgc tttcgcggat agaggttgac catgcgaaca gatacagcgc
    16501 ttcaacgtcg aagataacga agaacatggc caccagataa aacttggcgg acaggcgtaa
    16561 gcgggcggag ccgaccgagt cgataccgga ttcaaacgga acgttttttg acctcgcgcg
    16621 tgcgcgaccg cctaaaaacc aaccgcctac cagcatcagg caacacaggc caatggcaac
    16681 gataagaaag atagcgaatg cccagtgatg agcgatgact tcagtggatg ttgacatact
    16741 cattgcttac tcatcaaaag tagcgcctga ttctctgctc tttacggcag atggacgcca
    16801 catcgattca tggggaggaa taaaaaaaac cttacaataa ctgcagaaac tattttatac
    16861 agctaattga tgtggtcttt tactcctttc tataaccttt tgtcaacttt aacaaaggtt
    16921 tcttcacact gatttacaca atgacaacaa catttgcatt tggcaccctt tctccccaga
    16981 tcattgacga ctccaggaaa attagatgtt gttgaatcgt gtccgttgtg aagcaatgga
    17041 aaaaatacgg gtctattttg acaggaattt gtgtcgattc ctccccccaa aagagagtat
    17101 tttcttgatc tgtgacacgc ttttgtcatt ctataacaaa aatgcagcaa caaattcacg
    17161 tattttttaa cattattgta gcaggagatt tttttcaggt gattatttgt gcgtacgggg
    17221 cgtgaatctc tggtgattga aaaatgaaca gttttgtacg ttctgcacga tacgaggaag
    17281 gctttttact aaaaaaaagc cgctggggtt taaaataaac ccagcggttc gtttttacac
    17341 tattgtctca ggaattatct atcgtccgtc gattactcga tatccctttc aatcaacaat
    17401 gagtcatctc cttccggagc agacattggg ctgtattgcc acggattatg gtagctctcc
    17461 atcgcctgat agatcacctg ggccagctca ttattactgg acgggtcata gcacagcaaa
    17521 tattcggtat caggtagcgg cggtaagcca tctactccac tcagcacacg caggtccgga
    17581 ctcatcatct cgaccggcct tgccgtcacg ccaagaccgg ctttcactgc cgcacgaacg
    17641 gccggaagcg tcgatgcgac ataagccagt cgccagggaa tatctgcttt attaagcgtc
    17701 gccagcacca tatcgcgaaa cggacttgga tcatccagca acacaagagg gatcggctca
    17761 cctttttgca atacgtattc cgctgcgcag taccagtgtg ttggcgaggt acgcaggttc
    17821 aatgccttaa aggtagaagg acgatgggtg gttaccatca aatccacttc ctgggattcc
    17881 agcatttctg ccatgtaggc attacgttta acgcggacat ccagcgctag tttcggataa
    17941 accgaactca cgcgatttaa caggaaaggt aagatcgtat cggcagattc atctgaagca
    18001 ccgatagtta acacgccctg aagattactg aacattaatg atgagcaggc ctcatcatta
    18061 aaacgcagga ttttcctggc gtaaccaaga agttgaatgc catgttcagt taacagtttg
    18121 ttgcgaccgt gacgagcgaa cagttctttc ccaacgagtt gttccagacg ctgcatttgc
    18181 tgacttacgg cggactgagt acgacacaca gcggcagctg cggcagcaaa agtgttcaga
    18241 tcggcaacag caacaaatgt tctcagcaga tcgaggtcga ggttaattat cagacgattt
    18301 gcacttatca tatattatca cttactggcg gctcatactg agctggttaa tgctgtgcac
    18361 acacaaacaa gcaattccat ttgtaatgtg cctccctggc agtttcatcc ggaacccgga
    18421 cgaaagtaaa aatgcatatg agttgcacta aaaaagtgac tcacattgtt ccgttataat
    18481 gcctgaagta gatcacagaa tatatcttca gggatcgcat atctattaag ttactcactc
    18541 ttttctattt atgacatgcg cgtgtttgta taaatgtaaa tgtgagtcct tgttccactc
    18601 tcgtacagca tcgctggtca tacgcgaaca cgtacaaaca gcaatggtga ggcggcatca
    18661 agagcaggga tccgtcattt atgcaagcat tttaccccaa aaacctttta tttataaggg
    18721 tcattgcgaa ttatctgatg caaagttatg ttatgttagg caaagtaatc ttctattatt
    18781 aataagcaca acaaaacctt tttgaatatt aaataataat taattagcat catccccatt
    18841 cattaattcc gcttaacaat agtttcacgc cttgccccca ttgtgacacc tcggaaatca
    18901 tcacgctgtt aaactctccg taatgcggaa atatcatcat cgcgattcag tagtgaattc
    18961 ttaaacgagg cattttcaca caattatctt acagataaaa aaaccacact tacaattaag
    19021 aatcagaaca attcactaca taacattact tattaagcat atgcacctca ttattttgcc
    19081 attattaagt tattaacagc acaatcgagc cttcccctct ggcaaaatct tattctgcag
    19141 accttcaaaa caccgtcctg ggggagtaca ttgttctaag ctgacttcca cggcagggag
    19201 tggcgataac agcaaaaaag gtcaagattc atgtccccca ttgaaaaatc cagcaaatta
    19261 gagaatgtct gttatgacat ccgtggtccg gtgctgaaag aagcaaaacg cctggaagaa
    19321 gaaggtaaca aggtactgaa actgaacatc ggcaaccctg ccccgttcgg ttttgacgcg
    19381 ccagatgaaa ttctcgttga cgtgatacgc aacctgccta ccgctcaagg gtattgcgat
    19441 tccaaaggtc tttactccgc gcgtaaagcc atcatgcagc actaccaggc tcgtggcatg
    19501 cgtgatgtta ccgtggaaga tatttacatc ggcaatggtg tatcggagct tatcgttcag
    19561 gcaatgcagg cattgctgaa cagcggggac gaaatgttgg ttcctgcacc tgattaccca
    19621 ctatggaccg cagcggtttc gctctccagc ggtaaagcgg tgcattatct ttgcgatgaa
    19681 tcctctgact ggttcccgga cctcgatgat attcgcgcta aaattacgcc ttgtacgcgt
    19741 gggatcgtta ttatcaaccc aaataaccca accggcgcgg tatattccaa agagctttta
    19801 atggagattg tggagattgc acgtcagcat aatctcatta tcttcgccga tgaaatttat
    19861 gacaaaattc tctacgacga cgctgagcat cactccattg cgccgctggc acctgacctg
    19921 ctgaccatta cctttaacgg actgtcgaaa acgtaccgcg ttgcaggctt ccgtcagggg
    19981 tggatggtgt tgaacgggcc gaaaaaacac gccaaaggtt acatcgaagg tctggaaatg
    20041 ctggcttcaa tgcgcctgtg tgctaacgtt cctgcgcaac acgccattca gaccgcactg
    20101 ggtggttatc agagcatcag tgaatttatt acccctggcg gtcgtcttta tgagcagcgt
    20161 aaccgcgcgt gggaattgat caacgatatt ccgggcgttt cctgcgtgaa acctcgtggt
    20221 gcgctgtata tgttcccgaa aatcgacgcc aaacgcttta acattcacga cgatcagaaa
    20281 atggtgctgg atttcctgtt gcaggaaaaa gtcctgttgg tgcaagggac ggcattcaac
    20341 tggccgtggc cggatcactt ccgcattgtc acgctaccgc gtgtcgatga tatcgagctg
    20401 tctttgagca agttcgcgcg tttcctttct ggttatcatc agctgtaatc ttaatttcac
    20461 tgccggagat tgcatccggc agtgttatcc cgccacaatg acctgatgat gtcatcatac
    20521 gtaaggtcac tatgaaacag agccattttt ttgcccatct ctcccgcctg aaactcatta
    20581 accgctggcc gctaatgcgc aacgtgcgga cggaaaatgt gtccgaacac agtttgcagg
    20641 tagcgatggt cgcccatgcg ctggcagcta tcaaaaatcg taaatttggc ggtaatgtca
    20701 acgccgaacg tatcgcttta ctggcgatgt accacgatgc ttcagaagtg ctcaccggag
    20761 atctccctac tccggtgaaa tacttcaatt cgcaaatcgc tcaggaatac aaggctattg
    20821 aaaaaatcgc tcagcaaaaa ctggtcgata tggttccgga agagctgcag gatatctttg
    20881 cgccgttaat tgacgagcat gcatatagcg atgaagaaaa atcgctggtg aaacaggcag
    20941 atgcactgtg tgcatatctg aaatgcctgg aagaacttgc ggccggaaat aatgaattct
    21001 tgctggcaaa aacgcgactg gaagcgacgc ttgaagcgcg ccgcagccaa gagatggact
    21061 acttcatgga agtatttgtt cccagcttcc atctttcgct cgatgagatt agccaggatt
    21121 caccgctgta agcagccgga gtctgcgtcg catcaggcaa taagcaccgg atgcgacatc
    21181 aggctcttgt caaaacggaa acagcatcgg gatcatcacc acacaaaccg ccatcacgat
    21241 aatggtgaac ggtaccccca acttaacaaa gtcactgaag ctgtaattcc ccggacctaa
    21301 aaccagtgtg ttaacaggtg aagatatcgg tgtcataaag gcggcggatg ctgccatcgc
    21361 cacgaccatc gcaaatggat aaggcgacac ccccatcgtt ttggcagcag ccagcgcaat
    21421 cggagccatc aacaccgccg tcgcggtatt agaaataaat agcccaataa ccgccgacaa
    21481 gacaaacaaa caacccagca tcatatgtgg cccgtaaccg ccgccaatgt ccatcagccc
    21541 tttcaccacc agcgcgacac ctcccgtttt ctgtaatgcc acagcaaatg gcatcatccc
    21601 aacgatcaaa ataatgctcg gccagtgaat ggatttatag gcgctttcag catctataca
    21661 gcggaatttc cccatcagca ggcaggcgat aatagcggca acaggattag gaatttcatc
    21721 ggtcagcatt aacgccacca ttagcaccag acagaaaatc gcatggggtg cctggctgtg
    21781 cgcgggcgat gcttcactca cctcttccgg taagttcagc gctacgaagt cgcggccctg
    21841 tttggccagc ataccgatca gtttccagtt acccacaacc aggatgatat cgcccagcag
    21901 cagaggctca tccgccagcg aaccttccag cgccacgcca ttgcgcttta gccccactac
    21961 attcaatccg tagcgggtac gaaaaccaat ttcgcgcacc gatttaccaa taagttctga
    22021 ctcaggaatt aatgaaatct ctgccatgcc cacatcaagg gcctggtcag aaaaatactc
    22081 gccgcgcagt accatcggct ccagcaattg ctcactacaa aattgccgga gatcgacatc
    22141 agccgcagac atatcaataa gcaaaacgtc acgcgcgcga aattcagaaa ccccattaac
    22201 gttcacgata acgcgacgaa aacgccgcca gcgttcaaca ccgatgacgt tagcaccata
    22261 acgttcacgt aatttgagat cgtccagccg ttgaccaatc attggcgatc cggggcgaat
    22321 agccagacgt cgcgcacgcc cggtcagtcg atattcacgg ataagatcgc gaaaggttcg
    22381 gcgcgtccag ccttcgcgct gcggggtctg ggtatcccct ttcagcatga aacgcatcac
    22441 taacatatac aaaataccca gcaccagcac gaccaggcca ataggtgtta cgctaaagaa
    22501 actgaagccg tgatagcctt cacgcagcaa ttcactgttg actaccaggt tcggcggcgt
    22561 cgccaccagc gtcatcatgc cgcttatcag cccggcaaaa cttaacggca tcatcagacg
    22621 cgacggcgac gtttgcatac gcatggcaac gcttaacacc acgggaataa agatagcaac
    22681 aacgccggtt gaactcataa acgcgccaag ccccgcgacg gtcagcatca acaaaaccaa
    22741 cattttgatt tcactgttgc ccgccacttt gaccagccat gtccccatta cagtggcaac
    22801 accggtacgg accaaaccat cgccaataat gaacaaggcg gcaatcagga caacgttagg
    22861 atcggaaaag ccggaaaata cttcggggac cgtcagcgtt ccgcttaatg caaacgcgac
    22921 aataacaaac aaagcgaccg catccatacg cactctgccc gtcgcaaaca agacgatggc
    22981 aacggccagt aatgaaagaa cccaaatcaa ttcaccgttc acaacttatc cttgttaatt
    23041 gagggggata acttgattct gccataaaaa agccccgacg agacggggct aaatcatgat
    23101 caggtgtttc actgaataat aacatcgcca tttggctgtt tggtcacagt aatttgctcc
    23161 agactgtgga ggactaaatc gacttcattc aggcgcgggg tatctgccgg ggcattaacc
    23221 gcaataacat ggcaacccgc cgccagacct gaaagcacgc cagcgggagc atcttccacc
    23281 acaacacact cctgcggcgc aagccccagc agctgcgcgc ctaacagata cgcatcaggt
    23341 tctggttttc cgcgcttcac ccgctcagcg gttacaaaca cctctggcgc gggaagcccc
    23401 gctattttat gacgcgctcg cgctaccggc atggagccag aagtcacaat agcccacgga
    23461 atacctgctt tattcaaatg attgagtaag gcgattgccc ccggaagcgc ggtaatgcct
    23521 tcggtttccg tggcctcaat gtgctccaga cgcgtaaact cggcggcaat gtctgcctcg
    23581 gatttgcccg ccataaaatg gcgcagagag gtgatcgcct gtttaccgtg aatgaaagcc
    23641 agcacctctt ccggcgataa cccatgacgt ctggcccagt tgctccacgc ccgttctacc
    23701 gcaggcaggg aatccaccag cgttccatca agatcaaaca gaaaaccttt gcaccgcacg
    23761 cgggcctcct caggcgttaa taatttgatt aatttcgttg gcgctcaaat ggtactgacg
    23821 cgggcaggca tgccagacat taagcatgcg ctgatatttt tcccacattg gcgtctgggc
    23881 gttaaaaccg tgagttccag cgtcaaaatg ggtatagcgc ccttccacat taaccataaa
    23941 gcgaacataa ccgaggtaac gtgcttcagt ggcggcgtca aagccgagga aggtgacacg
    24001 acgttcatcg atggattgct ggtcctgcaa attagaccag gaaacatgca acgcatgata
    24061 catctccata atgtcgatga tagtgcggca ggtttcttct ttcagctcgc caaactcgcg
    24121 atccagttca cgcatctgta atccataacc acgctcaatg attgtttgca ggcgacggta
    24181 acgttcagca tttgccggat cgagcatagt catcatcttg tactggttag acaaaataag
    24241 acgttgcgcg ttggtcattt ccattattga ctcctgtatc actctactac ggtgaaaaaa
    24301 aagaaggctg agtatgcctt cttttatatg cgtaatcagg ggtcaattac aaatcatcaa
    24361 ggaaagtttt atccagttgt ttgaaggcac gcttaagcgt gtcagctaat gcctggtaat
    24421 caggcttgcc ttcaacgggt gccaacacct gtccagactc ctgcaattta ccacgaactt
    24481 cataaaacca gttgaggatt gcaggaggta atggcgttac agaacgcttg cccagccacc
    24541 acaatccctg catgggtaaa cttaaggcga acagcgcagt ggcaactgcc ggcccaagct
    24601 gaccgcccag ggcaatctgc cagcagagag taaatacggc gatcggcggc ataaaacgga
    24661 tcgcataacg cgtcatcttg ataacgcgat tttcgacaaa gactggggca aggcgttttt
    24721 ccagcggcca cgtctttgag taatgctgtc cccggcgaaa caagctaaaa aaattaacag
    24781 aacgattatc cggcgttgac atgcttcacc tcaacttcac atataaagat tcaaaaattt
    24841 gtgcaaattc acaactcagc gggacaacgt tcaaaacatt ttgtcttcca tacccactat
    24901 caggtatcct ttaacagcct gaaggcctaa gtagtacata ttcattgagt cgtcaaattc
    24961 atatacatta tgccattggc tgaaaattac gcaaaatggc atagactcaa gatatttctt
    25021 ccatcatgca aaaaaaattt gcagtgcatg atgttaatca taaatgtcgg tgtcatcatg
    25081 cgctacgctc tatggctccc tgacgttttt ttagccacgt atcaattata ggtacttcca
    25141 tgtcgagtaa gttagtactg gttctgaact gcggtagttc ttcactgaaa tttgccatca
    25201 tcgatgcagt aaatggtgaa gagtaccttt ctggtttagc cgaatgtttc cacctgcccg
    25261 aagcacgtat caaatggaaa atggacggca ataaacagga agcggcttta ggtgcaggcg
    25321 ccgctcacag cgaagcgctc aactttatcg ttaatactat tctggcacaa aaaccagaac
    25381 tgtctgcgca gctgactgct atcggtcacc gtatcgtaca cggcggcgaa aagtatacca
    25441 gctccgtagt gatcgatgag tctgttattc agggtatcaa agatgcagct tcttttgcac
    25501 cgctgcacaa cccggctcac ctgatcggta tcgaagaagc tctgaaatct ttcccacagc
    25561 tgaaagacaa aaacgttgct gtatttgaca ccgcgttcca ccagactatg ccggaagagt
    25621 cttacctcta cgccctgccg tacaacctgt acaaagagca cggcatccgt cgttacggcg
    25681 cgcacggcac cagccacttc tatgtaaccc aggaagcggc aaaaatgctg aacaaaccgg
    25741 tagaagaact gaacatcatc acctgccacc tgggcaacgg tggttccgtt tctgctatcc
    25801 gcaacggtaa atgcgttgac acctctatgg gcctgacccc gctggaaggt ctggtcatgg
    25861 gtacccgttc tggtgatatc gatccggcga tcatcttcca cctgcacgac accctgggca
    25921 tgagcgttga cgcaatcaac aaactgctga ccaaagagtc tggcctgctg ggtctgaccg
    25981 aagtgaccag cgactgccgc tatgttgaag acaactacgc gacgaaagaa gacgcgaagc
    26041 gcgcaatgga cgtttactgc caccgcctgg ccaaatacat cggtgcctac actgcgctga
    26101 tggatggtcg tctggacgct gttgtattca ccggtggtat cggtgaaaat gccgcgatgg
    26161 ttcgtgaact gtctctgggc aaactgggcg tgctgggctt tgaagttgat catgaacgca
    26221 acctggctgc acgtttcggc aaatctggtt tcatcaacaa agaaggtacc cgtcctgcgg
    26281 tggttatccc aaccaacgaa gaactggtta tcgcgcaaga cgcgagccgc ctgactgcct
    26341 gatttcacac cgccagctca gctggcggtg ctgttttgta acccgccaaa tcggcggtaa
    26401 cgaaagagga taaaccgtgt cccgtattat tatgctgatc cctaccggaa ccagcgtcgg
    26461 tctgaccagc gtcagccttg gcgtgatccg tgcaatggaa cgcaaaggcg ttcgtctgag
    26521 cgttttcaaa cctatcgctc agccgcgtac cggtggcgat gcgcccgatc agactacgac
    26581 tatcgtgcgt gcgaactctt ccaccacgac ggccgctgaa ccgctgaaaa tgagctacgt
    26641 tgaaggtctg ctttccagca atcagaaaga tgtgctgatg gaagagatca tcgcgaacta
    26701 ccacgctaac accaaagacg ctgaagtcgt tctggttgaa ggtctggtcc cgacacgtaa
    26761 acaccagttt gcccagtctc tgaactacga aatcgctaaa acgctgaatg cggaaatcgt
    26821 cttcgttatg tctcagggca ctgacacccc ggaacagctg aaagagcgta tcgaactgac
    26881 ccgcaacagc ttcggcggtg caaaaaacac caatattacc ggcgttatcg ttaacaaact
    26941 gaacgctccg gttgatgaac agggtcgtac tcgcccggat ctgtccgaga tttttgacga
    27001 ctccaccaaa gcaaaagtga acaacgttga tccggcgaag ctgcaagaat ccagccctct
    27061 gccggttctc ggcgctgtgc cgtggagctt tgacctgatc gcgactcgtg cgatcgatat
    27121 ggctcgccac ctgaatgcga ccatcatcaa cgaaggcgac atcaatactc gccgcgttaa
    27181 atccgtcact ttctgcgcac gcagcattcc gcacatgctg gagcacttcc gtgccggttc
    27241 tctgctggtg acttccgcag accgccctga cgtgctggtt gccgcttgcc tggctgccat
    27301 gaacggcgta gaaatcggtg ccctgctgct gactggcggc tacgaaatgg acgcgcgcat
    27361 ttctaaactg tgcgaacgtg ctttcgctac cggcctgccg gtatttatgg tgaacaccaa
    27421 cacctggcag acttctctga gcctgcagag cttcaacctg gaagttccgg ttgacgatca
    27481 cgagcgtatc gagaaagttc aggaatacgt tgctaactac atcaacgctg actggatcga
    27541 ttctctgact gccacttctg agcgcagccg tcgtctgtct ccgccagcgt tccgttatca
    27601 gctgactgaa cttgcgcgca aagcgggcaa acgtatcgtt ctgccggaag gtgacgaacc
    27661 gcgtaccgtt aaagcagccg ctatctgtgc tgaacgtggt atcgcaactt gcgtactgct
    27721 gggtaatcca gcagagatca accgtgttgc agcctctcag ggtgtagaac tgggtgcagg
    27781 cattgaaatc gttgatccag aagtggttcg cgaaaactat gttggtcgtc tggtcgaact
    27841 gcgtaagaac aaaggcatga ccgaaaccgt tgcccgcgaa cagctggaag acaacgtggt
    27901 tctcggtacg ctgatgctgg aacaagatga agttgatggt ctggtttccg gtgctgttca
    27961 caccaccgca aacaccatcc gtccgccgct gcagctgatc aaaactgcac cgggcagctc
    28021 cctggtatct tccgtgttct tcatgctgtt gccggaacag gtttacgttt acggtgactg
    28081 tgcgatcaac ccggatccga ccgcagaaca gctggcagaa atcgcgattc agtccgctga
    28141 ttccgctgcg gccttcggta tcgaaccgcg cgttgctatg ctctcctact ccaccggtac
    28201 ttctggtgct ggtagcgacg tagaaaaagt tcgcgaagca actcgtctgg cgcaggaaaa
    28261 acgtcctgat ctgatgatcg acggtccgct gcagtacgac gctgcggtaa tggctgacgt
    28321 tgcgaaatcc aaagcaccga actctccggt tgcaggtcgc gctaccgtgt tcatcttccc
    28381 ggatctgaac accggtaaca ccacctacaa agcggtacag cgttctgctg acctgatctc
    28441 tatcggaccg atgctgcagg gtatgcgcaa gccggttaac gacctgtccc gtggcgcact
    28501 ggttgatgat atcgtctaca ccatcgcgct gactgcgatt cagtctgcac agcagcagta
    28561 atctcgtcat catccgcagc tttgcgctgc ggatatctga accggaaata gtcactattt
    28621 ccggtttttt attctcttaa tttgcattaa tcctttctga ttatcttgct taactgcgct
    28681 gcatcaatga attgcgccat ctcactttgc atacttacca ctttgttttg tgcaagggaa
    28741 tatttgcgct atgtccgcaa tcactgaatc taaaccaaca agaagatggg caatgcccga
    28801 tacgttggtg attatctttt ttgttgccat tttaaccagc cttgccacct gggtagttcc
    28861 ggtgggcatg tttgacagtc aggaagtgca gtatcaggtt gatggtcaaa caaaaacacg
    28921 caaagtcgta gatccacact catttcgcat tctgactaac gaagcaggcg aacctgagta
    28981 tcaccgcgta cagctgttca cgacgggcga tgaacgcccg ggtctgatga acttcccgtt
    29041 tgaagggtta acctcaggat cgaaatacgg gacagccgtt ggcatcatca tgtttatgct
    29101 ggtgattggc ggcgcgtttg gcattgtgat gcgtacagga accattgata acggtatcct
    29161 ggcgcttatt cgccataccc gcggaaatga aattctcttt attcctgcgc tgtttattct
    29221 gttttcactt ggcggcgcaa tatttggtat gggagaagag gccgtcgcct ttgccattat
    29281 catcgcaccg ctaatggtcc ggctgggcta tgacagtatt accaccgtcc ttgtgaccta
    29341 tattgccacg caaatcggtt ttgccagttc gtggatgaac ccgttttgtg tggttgttgc
    29401 tcagggtatt gccggcgttc cggtgctttc tggctccggg ttgcgcatcg tggtgtgggt
    29461 tatcgccact ctgattggcc tgatctttac catggtgtac gcctcacgag tgaaaaagaa
    29521 tcctcttctg tcacgcgtgc atgagtccga ccgcttcttt cgtgaaaagc aggctgatgt
    29581 tgaacaacgt ccgtttacct ttggtgactg gctggtattg attgtcctga ccgccgtaat
    29641 ggtctgggtt atttggggcg tgatcgttaa tgcctggttt attcctgaaa ttgccagcca
    29701 gttcttcacc atgggtctgg tgattggcat catcggcgtt gttttccgcc ttaacggcat
    29761 gacggttaat accatggctt catcctttac cgaaggggcg cgaatgatga tcgcccctgc
    29821 cctgctggtg ggtttcgcca aagggatttt gctgctggtc ggtaatggtg aagcgggtga
    29881 tgccagcgtg ttaaatacca tcctcaacag cattgccaat gccattagcg gtctggataa
    29941 cgcagtcgcg gcctggttta tgttgctctt ccaggcggta tttaatttct tcgtgacgtc
    30001 cggttctggt caggcggcgt taaccatgcc gttactggca ccgcttggcg atctggtcgg
    30061 tgttaaccgt caggttaccg tgctggcttt ccagtttggc gatggcttca gccacatcat
    30121 ttacccaacc tcagcttcgt taatggcaac gctcggtgtt tgccgggtgg acttccgtaa
    30181 ctggctgaag gtgggtgcga ccctgcttgg actgctgttt attatgtcca gcgtcgtggt
    30241 gatcggcgct cagttgatgg gctaccacta aaaatgttaa gagccgcgat tgcggctctt
    30301 tttttcattc tgccgtttca gtctctacgg cttcattttt ggcattgcgc ttcatccaca
    30361 acgccagcgc tttcagcgag tctggagtga actcatcgca gcgtgcggtg atttcttccg
    30421 gcgtcagcca gcaaacttca ctgacttcat cttcctgtag agcgaaggga ccgtgagaga
    30481 cgcagctgaa caacgcgccc cagacacggc aatttttatc ttcgaaatag aactgcccgt
    30541 gctcggcaaa ggggaccccg gcaatgccca actcttcttc cgcttcgcga cgcgcggatt
    30601 ccagcagttg ctcatcggcc tggactacac cgcctgcggt cgcatctaac atgccgggta
    30661 aaaagtcttt tgtctcggta cgacgctgga ccagaatttt gcccatgcca tcatgcacga
    30721 cgatataagt tgcacgatga cgcagacact gtgcccgcat ttgttcccgg ctggcttgtg
    30781 caatgacttc gttctcttca ttgacaatat ccacccattc agtacttgcc aaacgacgct
    30841 gttccattat caggaaacct tctttttctg gcgctcttac ggcgcatttt gagttgtggg
    30901 taaattacgg attaatcgcg acctgcgcaa tgatactttg atcattgagt gcgattacac
    30961 taagtacgtc attatccagc atgccataac tcgccggatt accgcctttc ggaatactca
    31021 ccgagccggg gttgaagtgg aaaatctccc cccgctgttc tgccactggc agatgggtat
    31081 gaccgtacac cagcacatcg ttctggttta aagcaggtag attttccggg ccaaaaagat
    31141 gaccatgcgt caaaaacaga cgttgttttt ccagtaatac ctgttgccac ggcgcggtta
    31201 tcgggaaatg cagcagcatt tgatccactt cgctgtcgca gttgccgcgc acagcgataa
    31261 ccttatgtgc cacttcatta agccgttcag cgactttggc tggcgcgtaa ccctccggta
    31321 aagcattacg cgggccatga ttcaacacgt cgccaaggat caccagccac tgggcaccgc
    31381 tttggacaaa caactccaga acacgttccg tcgccggtaa cgacccatga atgtccgatg
    31441 caaacatcag tttcatcact cactcctcgt cgaaaaaacg taagctatga tactggattc
    31501 tgtcgttgct atcagccaga ttgcttcgcc gaaagtgcaa taaaacgctg gacagacgct
    31561 cgctgccact ggaatgtcgc ataatccacc aggcgttccg gcacctcatc gccatgtagt
    31621 accaggcggt taatcattag cgccagatca gtatcagcaa tgcaccattc accaaataaa
    31681 ttcggctgac ccagtgctaa caaatgctct gccatcgcga acaatttctc tgcactggct
    31741 tttccctcgg ccgttagtgg cgcttttttc gcccccgcaa agacaacatc cgtcgggcgt
    31801 tcttcgcgaa tgggcatcag atcgctgcgc aaccaagcct gaatctgtcg tgcacgcgca
    31861 cgattttcta aatcaagcgg ataaatacgt tcccaggtcg gtggcgcaaa tcgatcttcc
    31921 agatactccg caatggcaga agattcactc aactcaaaat catcgatttg taataacggc
    31981 acacggcgtg tctgaccgta accttgccac gccggctgca aatgttcacc gctgtcgagg
    32041 tcgatggtct tgatatgaaa cgacaggcct ttctcctgca acgccaccca ggcggataac
    32101 acataagggg agaaaaaatg ggcatctgac caaagcgtga tagcggtttt actcattatg
    32161 tcctcggcaa taaagggggc ttgctatcaa actatagagc ctttttgtca ctgtcacgtg
    32221 acgaatactc acgcaagtcg ccgcgctttc tatacttgtg gtgttcagat gattagacaa
    32281 ccggagctgc aatgatcgat ctctatttcg ccccgacacc caatggtcac aaaattacgc
    32341 tgtttctcga agaagcaggg ctggattatc gcttaataaa ggtagacctg gggaaaggag
    32401 gtcagtttcg cccggaattt ttgcgcattt cgcctaacaa caaaattccg gcaattgttg
    32461 atcattctcc agccgatggc ggcgaaccgc taagcctctt tgagtctggt gccattttgt
    32521 tgtatctggc tgagaaaacg ggactctttt tgagtcatga aacgcgtgaa cgcgccgtca
    32581 cattacagtg gttattctgg caggtaggcg gactggggcc gatgcttggg caaaatcatc
    32641 attttaatca cgcagccccc caaaccattc cttacgctat tgaacgttat caggttgaaa
    32701 ctcagcgtct gtaccatgta ctgaacaagc ggctggaaaa ttcgccctgg ctgggaggcg
    32761 agaactacag cattgcggat attgcctgct ggccgtgggt taatgcctgg actcgccagc
    32821 gaattgacct cgcaatgtat ccggcagtca agaactggca tgagcggatc cgttcgcgcc
    32881 ctgccaccgg gcaggcactg ctaaaagcac aacacggtga tgagcgttcg gatagttaac
    32941 agaaacaggt tctcgtgtat tatttcattc taagtaaaac aacggagaac ctgcaatggc
    33001 acaacctgcc gctattattc gtataaagaa ccttcgttta cgtacgttta tcggaattaa
    33061 ggaagaagaa attaacaacc gtcaggatat tgttatcaat gtgacgatcc actaccccgc
    33121 cgataaagcg cgcactagcg aagatatcaa cgatgcgctg aattatcgca ccgtaacgaa
    33181 aaacattatt cagcatgtag agaataaccg tttctctttg ctggaaaaat taactcagga
    33241 tgtgctcgat atcgcacgtg aacatcactg ggtgacgtat gctgaagtgg agatcgataa
    33301 actgcacgcg ctgcgctacg ccgattcggt atccatgacc ttaagctggc agcgttaacc
    33361 gccatatcgg gaggctgcat gaacatagtg atcaccggag ggacgggatt aattggtcgc
    33421 catttgattc cacgtttgct ggacctgggc catcaaatca cggtagtgac gcgtaacccg
    33481 cagaaagcca gttccgttct cggccctcgg gtgacactat ggcaagggct tgccgaacaa
    33541 agcaacctca acggcgttga tgcggtaatc aacctggccg gagaaccgat tgctgataaa
    33601 cgctggactc acgagcaaaa agagcgtctc tgccaaagcc gctggaatat cacgcaaaaa
    33661 ctggtcgatt tgattaatgc cagcgacacg ccaccgtcgg tactcatttc cggctcggca
    33721 acgggctatt atggcgactt aggtgaagtg gtggttaccg aagaggaacc gccgcataac
    33781 gaatttaccc ataaactctg cgcccgctgg gaagaaattg cctgccgggc acaaagtgac
    33841 aaaacgcgag tgtgcctgct gcgtaccggc gtagtgctgg caccggatgg cggtattctc
    33901 ggtaaaatgc tgccgccgtt tcgtcttggc ctgggcgggc cgattggttc cggtcggcag
    33961 tatctggcct ggattcatat tgatgatatg gtcaacggca ttctctggct gctggataac
    34021 gagctgcgcg ggccatttaa tatggtttcg ccctacccgg tgcgcaatga acaatttgcc
    34081 catgcactgg gtcatgcgct gcatcgcccg gccattttgc gcgtccctgc gaccgccatt
    34141 cggttgttaa tgggcgaatc ttcagttctg gtattaggcg gtcaacgcgc gctgccaaaa
    34201 aagctggaag aagcgggttt tgcgtttcgc tggtacgatt tagaagaggc gctggcggat
    34261 gtcgttcgct gatgtggttt actgcaaaca tccgccagtt aactcccggt gttacagggt
    34321 tagtggcttt gcgcgataag atcgtctggt gaaagtcggg tcagcgtcag gatgacttcg
    34381 tggtcaaatc ctttttccat catctcctga gcaatgcgca gggcttcttc gtgtttgccc
    34441 tgcattgcgc cttcttcacg taatctgtca gcaatggtca tcagtttctc cttttcttgt
    34501 ggtgcgcgtt ccgctatctc accaataaat gcacggaaac gccgggcatc ccccgtttgt
    34561 aatacgtaat taaacagggc ttttagctgt ctgtcattag tgttccctgt aactagcagc
    34621 gaaacaattt ggtcgactaa tcccaacaga tcgcgctgac gaatatgttt ctgaattaac
    34681 tccaacagcg ccattttgcg atgttgcata atctcgtcat ccggcaccac ggtaatatcc
    34741 accagcggaa aagccgatga ataaattttg cgggcaatcg caggctcggc aaattcatca
    34801 agccagcaga gtgaatatgg ataagggctt ctgcaaccat gataaaacag catcgggatc
    34861 actaatggaa gctctttata acccgcatca agatggtttt gcatcgccgc aatggaatag
    34921 cgcatcatgc gaaaagccat taattcctct ggcttacttt ggtgctctat caccacataa
    34981 atataacccg ctccctcctg cgttttcaca gaccacaaga ggtcggaata atattgccgc
    35041 aggtcttcat caataaaact gtttggttcc agtttaagcg tcgttaaatc acacagtttg
    35101 cgcagcggcg cgggaagatg aatatcaata aaatcccgcg cggtgtctgg atggcgtaaa
    35161 aaagatttaa ataccgcatc atgcggcgtg gaagttgtcg atattgtcat ggcgttccgt
    35221 cacccctcaa atttaagatg acgcgacaat aaccacgatt tacacccaat gcagcaggca
    35281 gtttttatct ttggacagcg aattcagaac tatttttagc ggactacaac ttttacattt
    35341 cagcggaagc ggactcgcaa attccccatg aaatactccg gcagcgttaa ccaccggaga
    35401 ggaaattatt tcagcgaacc tttaaggaac tgttgcagac gagggctttg cgggttaccg
    35461 aataactgct ccggcgcccc ttcttcttct attttcccct gatggaggaa aatgacgtga
    35521 gtagaaacat gacgagcaaa gcccatttcg tgagtcacta ccaccatggt tttcccctct
    35581 tctgccagtt gctgcataat acgcaacact tcgcctacca gttcaggatc gagcgctgaa
    35641 gtaggttcat caaacagtaa aacttccggt tccatcgcta gcgcccgcgc gatagaaaca
    35701 cgctgttgct gaccaccgga caggtgtacc gggtatttcc cctgcgcacg ttcgtctatc
    35761 ccgacttttg ccagatattt caccgcccgc tcgcgcgctt cctgcttgct caggcccaac
    35821 acctgaatcg gcgcttccat gacgttttcc agcaccgtca tatggctcca gagattgaag
    35881 tgctggaata ccatcgtcag gcgtgtgcgc aataagcgca gctgattttt atcggcgact
    35941 ttgagttgac cgtctttatc gcgcaccaga ttgatcgtct ggccattgac cacgatcgat
    36001 ccttcgctcg gtttttcgag gaagttaatg cagcgcagaa aggtactttt acccgatccc
    36061 gacgatccga tgatgcttat gacatctccg gcattcgctt gcaatgagac ccctttcagc
    36121 acttcgtgtt cgccgtagcg tttgtgcaaa tcgataacgt ttaatttatt ctcggacatc
    36181 gtattctcag tgcgttgaag aaggtttcac atgctgcaac cagcgttttt cagctctacg
    36241 aaagaggctg atcaggacat aagagatgat taaatagagc accgcggcaa tgccgaaagc
    36301 ggtaaaaggt tgatatgtgg cggcgttaat atcgcgggca attttcagca gatccggcac
    36361 cgtggcagta aatgccagcg cagtagagtg cagcatcagg atcacttcgt tgctgtacgc
    36421 cggtaacgca atacgcagcg cagaaggcaa aataatgcag cgatacattt taaaggtcga
    36481 gaagccatag gctctggcgg cttcaatttc cccatgcggc actgaacgga ttgccccggc
    36541 aaaaatctcg gtagtgtaag cgcaggtgtt aagcgtcagc gccagcacgg tacagttcag
    36601 accactgcga aaaaaggcgt taaggaactc ggttccctta acaatctcca gcgtgtacat
    36661 gccggaatag aacaccagca actgaacata cagcggcgta ccgcgaaaaa tataggtaaa
    36721 taaccagatt ggaaactgga tgtatttatt acttgagacg cgaccaatcg ccagaaacag
    36781 cgccagaact ccgcctatca ctaccgacaa aatcagcagc cacagggtga tcgccacgcc
    36841 agtaaagcga taaccgtcag tccacagcag tggtttccag tattcatgta agatttcgat
    36901 cacaggtcag ccctcttcac acccacggag tagcggcgct caaggaacag cagcacacca
    36961 ttggaaacgg tggtgaaaac aagataaatc acgccgcaga cgatagcgaa atagaacggt
    37021 tcccaggtac ttttgcctgc cagctgcgtg gctttgacca catcttccag gccgagtaac
    37081 gaaaccagtg cggtagattt gaggatcacc tgccagttgt tgccaatgcc tggcagggcg
    37141 taacgcatca tcgccggaaa catgatccgc cgaaacactt gctcacgagt aaaaccgaac
    37201 gccgtcgccg cctctatatg tcctttcggc actgccataa acgcgccacg aaacgtttcg
    37261 gtaaaataag caccgtagat aaaaccgaga gtgataatac ctgcgaccat cggatcaata
    37321 tcaatctgcc cgacgcccat cgcctccgtc accgtgttta gcgcaatctg caaaccgtag
    37381 aaaatcagca gcatcaatac taaatccggc acgcctcgaa tcagcgtggt gtacccttcg
    37441 aaaataaggc ccgaaagccg attttgtgag agcttaccgc cagcgccaat taaaccgatg
    37501 attacagcga gcactacaga gctgatagcc agctccagcg tgacgagcgc accctgtaaa
    37561 ataacacctg aaaacccata caacatgctg cctgtcctgt cgtgtgtggt gaattaccgc
    37621 ctcttgtctc cctccggata cccgaaggga gcgggctttc tttcaccgat ggcgattaac
    37681 caccataaac atcaaaatcg aagtactttt tcgctaattt ctcgtaagta ccgtcagcgc
    37741 gcatttcggc aaaggctttg ttcagtgcct cgcgcagttc gttatcttct ttacgcaggc
    37801 ccatgccggt ccctacgcca aacagttttt catctttaac agacgggcca ccgaatttgt
    37861 aatctttacc gacgggttgt ttgaggaaac cttcgctggc tgcgacctca tcctggaacg
    37921 cggcatcaat acgtccggca gtcaggtcag aataaatatt gtcctgcccc tgataagaaa
    37981 cgatttcaat gccttttggt gcccagtgtt cgttaccgaa cgtctcctgg gtggtgccct
    38041 gcaatacgcc tatccgtttg cctttcagcg attcgactgt cggctgaatg tcggaatttt
    38101 tcgccaccac caaacgagaa tcggcagcgt acagtttgtc ggtgaaggct atttcttgct
    38161 gacgtttttc cgtaatggaa agcgatgaca tgatggcgtc aatcttcttc gcttttaagg
    38221 acgggattaa cgcatcgagt gggttttcga caaacgtaca ttgcgtattg atgcgtttgc
    38281 acaactcctt cgccagatcg atatcgaagc caaccagttc accttgtgaa ttttttgatt
    38341 caaatggcgc ataggtcgga tcggtaccga tgcggatgtt ttgcggaatc gcagcaaatg
    38401 ccgcagttgc gctggagaag gccagaacca gagagagcga tagcaccagt tttttcataa
    38461 tgtcctcaac tgacagtctt tttatcggga atatttacag gtttgttaca cttatcgtgc
    38521 cataaaattg accatcaagg caaaatatcc cgccccgcta gacgtattat ttttcattaa
    38581 aaatgcttaa gtatgcattt caatgcacca cgatagtgca acaaacctgg cgtgcaccat
    38641 gatgatcatt ccatcaggta cagcttccct gcgacgtatc agtcaccgta gacattaaag
    38701 tcgaaatact ttttcgccat cttgtcgtag gtaccgtcct gacgcagctc gccaagcgcc
    38761 ttattaaacg cagccgtcag ttcagcatca tctttacgca gccctacacc ggtgccgtca
    38821 ccgaagtatt ttttgtcttt cactgatggg cctgcaaagg cgaaatcttt accggcgggt
    38881 tgcttaagga aaccttcgct ggcagcaact tcatcttgta acgcagcatc cagacgtcct
    38941 gcagccagat cggaatagac taaatcctgg ttggcatagg ccaccacatc cacgcctttg
    39001 ctacgccagg tctcgttagc gtatgcttcc tgggttgatc cctgcagcac accaacatgt
    39061 ttacctttca gtgaatccag cgttggctga atcggtgaac ctttggcggc aatcaaacga
    39121 gaatctgcgg cgtacagctt gtcagagaag gctatctcct gctgacgttt atcggtaatg
    39181 gagagcgacg aaataatggc gtcgattttt ttcgctttca gtgaggggat cagcgcgtca
    39241 aagtcactgg caacccaggt acatttcacc tgcatccgtt tgcacatctc gttaccgaga
    39301 tcgatatcaa agccaacaaa gtcacctttg gcatctttcg atgagaacgg tgcgtaggtg
    39361 gtatcggttc cgatacgtac cgtctccggt agcgccgcat agctggaagc cgctgcggag
    39421 agaccgacta acaaagacag agcgagaatc gacttcttca tacataaccc tcaagtgaaa
    39481 atggcttttt tatgttgtgt attgtgttgt gtgtttgcag gctctttcat gcaggtctta
    39541 tgccatcttg acgacagcag taacattcaa cgttaaatat gttaataaga cgttgcatta
    39601 ttgtcctaaa gttgaagata gcaggtatag cggttgaatc gcagcgtttc gattgaatgg
    39661 cagaaacaaa atgtcgagga tttgatcgcg gttgcaaaat tgccctgaaa cagggcaaca
    39721 gcggagttat gcgccctgcc agcgggcaaa gagatcttca ggaagggtta ccgcaaactg
    39781 gtcaagaaca cgattaaccg tctgatttat cacatcatca agggattgtg ggcgatgata
    39841 aaacgccgga acgggaggca taatcaccgc accgatttct gccgcctgag tcattaaacg
    39901 cagatggcct aagtgcagtg gcgtttcacg cacgcagagc accaacgggc gacgctcttt
    39961 cagcaccaca tctgccgcac gggtcagtaa gccatcagta tagctatgga caatgccgga
    40021 cagggttttg attgaacagg gtaaaatcac catccccaac gtctggaaag aaccggaaga
    40081 aatgctggca gcaatatcgc gcgcatcgtg tgtcacatca gctaacgcct gcacttcgcg
    40141 cagggaaaaa tccgtttcga gggataaggt ctggcgcgcc gcctggctca ttaccagatg
    40201 cgtttcgata tctgtgacat cgcgcagaac ctgtaataaa cgcacgccat aaatcgcgcc
    40261 gctggcaccg ctgatgccta caatgagtcg tttcataaat agttgccctg tcagacttgc
    40321 aggcagactt tgcaggattt cggcgggagt tgcaagtcag ggtgccagac cggcaccctg
    40381 agtgaaggca tcatccttcg ttatgcattt cgagattttc cacttcgttc tgacgttgca
    40441 cggctttggc gtcgtcatta cgtaaagtat cgaggaaatc gaggtagccc tgatcaacgt
    40501 ctttggtgac gtagacgccg ttgaacaccg agcattcaaa ctgctggata tccgggtttt
    40561 cagcgcgaac ggcttcgatc agatcgttca gatcctggaa aatcaacccg tcagcaccga
    40621 tgatctggcg aatttcatct acttcgcgac cgtgagcgat cagttccgtg gcgctcggca
    40681 tatcgatacc gtaaacgttc gggaagcgaa tttccggtgc cgcagaagcg aggtacactt
    40741 tcttcgctcc ggcttcgcgt gccatctcga taatctgctc agacgtggtg ccacggacga
    40801 tagagtcgtc gaccagcagg acgtttttat cgcggaactc ggcgcggttg gcgttcagtt
    40861 tacggcgcac ggacttacga cgcagctgct gacccggcat gatgaaggtg cggccaacat
    40921 agcggttttt aacgaagccc tggcggtacg gtttgcccag aatacgcgca atttccagcg
    40981 cgatatcaca cgaggtttcc ggaataggga tcaccacgtc gatatccaga tcttcccatt
    41041 cgcgggcaat tttttcgccc agtttggtgc ccatattcac gcgcgcgctg taaacggaaa
    41101 ttttgtcgat gaacgagtcc gggcgggcaa agtatacata ctcaaacagg cacggattgc
    41161 tgaccggatt gtcagcacat tgacgggtaa acaactgtcc ttcttcagtg atgtaaatcg
    41221 cttcgcccgg cgcgacgtca cgcaggaaat caaagcccag cgtatcgagc gcaacgcttt
    41281 cggaagcgac catatattct gtgcggttct cgtcaatatc acgttttccc agtaccagcg
    41341 gacgaatccc gttaggatcg cggaaagcaa ccataccgtg gccgatgatc atcgccacac
    41401 aggcatacgc gccgcggatt aagcggtttg tagcagcgat ggcggcgaaa atgttgtcgg
    41461 cttccagcgg gtagtggcgg aagttgtcca gctcgctggc gaagatatta agcagaattt
    41521 ccgagtcgga agtggtgttg atgtggcggc gtttttcttc aaacagtttt ttacgcaact
    41581 cgtgagcgtt ggtcagattg ccgttgtggg caagcgtaat gccatacggg gagttaacgt
    41641 aaaacggctg cgcttcagag gcgctggagc tgccagccgt ggggtaacgc acatgaccaa
    41701 tgcccatatt gccctgcaaa cgctgcatat ggcgagcttc aaatacatcg ctcaccagcc
    41761 cgttcgcttt ccgcaaacgg aagcagttat tggcatctat ggtgatgatg ccggcggcat
    41821 cctgaccgcg atgctgaagc accgttaagg catcataaat cgactggtta accggcataa
    41881 caccggcgat accgacaata ccgcacatac gtctttttcc tcgttaagcc acatctcaga
    41941 gcacttacgc tctgggcaag aaacttgacg agctttgcag ataatcaaaa aaccatctga
    42001 tgataaaact gaactgcggg atcagctgtg atttgctcca gtcttcgctt ttcgacaccc
    42061 cggtaaagga gtcgagaaag aagagaatgg cagcaacaat caacacaccg cgcaacgcac
    42121 cgaaacagac gcccaacacc cgatcggtgc ctgacaaccc cgttttctcc accaactggc
    42181 ctatcacgaa gttcacgata gcaccaacga tcagggtagc gataaacagt accgcgatgg
    42241 caatcccatt tcgaaccagt tcgtcttcaa agcccgtaaa ccagactgac aggtaagtgt
    42301 agtaatgact ggcaacaaag aaagcacaac cccatgtcac cagcgataac gcttcacgaa
    42361 caaagccgcg gatcaggcta accagagagg aaaaagcaat caccgcgatt atggcgtaat
    42421 caatccagac catatgtgtc ccacgatttt acgccctgtc atcctgttcg gggcgcattc
    42481 taacagaaaa agaaaacgtt tgcgtaggga tttccttccc gcgcatcaat aaaaatggcg
    42541 ctgaaaaaat attcaacgcc atcgactttt tatgcctttg cggcatcggg caatgcgtgt
    42601 cggatgccgc atgaacgcct tatccgacct acagttctgc tccagcacag gcctgataag
    42661 acgcgctggc gtcacatcag gcaacggctg tcggatgcag cgtgaacgcc ttatccgacc
    42721 tactgttcta ctcctgcgtt ggcctgataa gacgcgctag cgtcgcatca ggcaacggct
    42781 gtcggatgca gcgtgaacgc cttatccgac ctactattct acccctgcgt aggcctgata
    42841 agacgcgttg gcgtcgcatc aggcaacggc tgtcggatgc ggcgtgaacg ccttatccga
    42901 cctacggttc tacccctgca caggcctgat aagacgcgct ggcgtcacat caggcaagac
    42961 cgtattaatt cggtgtatag cccattacca cgccacttaa gccagaaagc tgcttgagct
    43021 cacccagcga tcctttcagc ttctctttgg aagcatccgg cccaaccaga atacgggtaa
    43081 ttttaccctg cactggcgtg gatggcgacg tataaacccg ataaccggca ccgcgcagct
    43141 taccgacaat ctcattcact ttatcggcat ttttcagcgc gcccagttgc acaacataag
    43201 ctttacccgt tggcgctgct ttttcttcca cgaccggctt cggttctggc ttcggcgcag
    43261 gtggtgcttc aacctttggc ttaagcggct ccaccggttt cggcttcggt ggggcgaccg
    43321 gtgcaggttc cggttcaaac tcggtgttat tggctgcaat agtggccgga tcgagcgacg
    43381 gtgctgcggc atcacctgcc cgtacctctt ccgctgcgcc ttccggcggc tgcgtcggca
    43441 acgcctgggt ggcggctggc atcatatcag gctcatcgcg atcgcccgct tttggcacca
    43501 gcgggatagc cgcgaactca tcctgataat gttttttctg cccgtccagc agccctggaa
    43561 gtacaatcac ccccagcgct accagcacga tcgtgcccac taaccgattc tgaaacttac
    43621 ttgccaccgc ttctcctcgc gtcaatcact tccatgacat gtgcgaccgt gtggaacgaa
    43681 ccacacacca gcacggtgtc ttccgcttta gcgtccgcca ttgcggcatc ccatgcctgc
    43741 gcaacgctat caaatgattt gccgttaccc aaatgctcaa gcagttgttc tgccgtggca
    43801 ccgcgcggcc cttccagtgg cgcacaatac cagtcatcaa ccacgctttt caaccaggcc
    43861 agagttccgg caatatcttt atcatgtagc ataccgataa ccgccagcac gcgcccgttt
    43921 ttcggtagcg ctttcatacg cccggtgaga tattccgccg catgtggatt atgcgcgaca
    43981 tcaaaaataa cgcgtggcga ctcgctcaca atctggaaac gtcccggcaa aattgcgctg
    44041 gcaatcccgt cgcgaatggc attttcactg acctccagcc cgctggcacg cagtgccgcc
    44101 agcgccgttg cggcattcgg ttgcgggaca agcggcaacg gcagattttc cagcgtgccg
    44161 tgagcatcgc taaacgtcca gtcatgatcg gtgacggaat agttccactc aacgccccga
    44221 cgttgtagca gtgcaccttt ttcctgcgcc acatcagcaa tggtagaagg catctccggc
    44281 tcaccgacaa ttgccggttt ttcgctgcga aagatacctg ctttctcgcg accaatactt
    44341 tcacgatctg gccccagcca gtcggtatga tccagcgcaa tactggttac taccgcgaca
    44401 tcggcatcga caatattggt tgcgtccaga cgaccgccca gccctacttc cagaatcact
    44461 acgtcaagtt gtgcctgctt gaacagccac aacgccgaca gcgtaccgta ttcgaaatag
    44521 gtcagggaaa tatcaccgcg tgccgattca atctccgcaa aagaagcggt gtgggccgat
    44581 tccggcaatt cctgaccctg cacacgtacg cgctcggtat aacgtaccag atgcggcgaa
    44641 ctgtagacgc ccactttgta ccctgccgcc atcagaatcg actccagtgt acggcaggtg
    44701 gtgcctttgc cattcgtacc cgcaacggta aacacaaatg gcgctggttt cagaacgcca
    44761 agacgcgccg cgacctggct cacgcgctca aggccgagat cgatagtttt actgtgtagg
    44821 ttttccagat aagaaagcca cgaagccaga ggcgacgcgg cttgaggagt gcgtttgata
    44881 atcatggtat ccgctgattc gttacggtga gaatagcaaa agggcagagc cagtggccct
    44941 gcccttatca gttatcaggc ctcaggttcc tgatccggta ccggtggcac cactacgcct
    45001 tcacgcggcg cttcaggatt cggcgctggc agattcatca acttcgccag aatgctcgcc
    45061 agtttcaggc gcatttccgg acgacggatg atcatgtcga tcgcgccctt ctcgatcagg
    45121 aattcactgc gctggaatcc tggcggcagt ttttcgcgaa cggtctgttc gataactcgc
    45181 ggaccggcaa agccaattaa cgctttcggt tcagcgatgt tgagatcgcc cagcatggcg
    45241 aaacttgcag aaacaccgcc catggtcggg tcggtcagca cggagatgta cggcaagccg
    45301 cgctcctgca tttttgccag tgccgcagag gttttcgcca tctgcatcag cgacatcagt
    45361 gcttcctgca tacgtgcgcc accagaggcg gagaaacaga tcagtgggca gttatcttcc
    45421 agcgcctgct caacggcacg cacgaaacgc gcacctacca cagaccccat ggaaccgccc
    45481 ataaaggcga actcgaatgc cgcagcgaca accggcattc catacagagt gcctttcatc
    45541 accaccagcg catctttttc gccggtttct ttctgcgcag atgccagacg gtctttatac
    45601 ttcttggagt cacgaaactt cagcacatct ttcggctcaa gctcgctacc cagctccaca
    45661 aggcttcctt catctaacag gctatgcagg cgattacgcg ctgtcatacg catgtgatgg
    45721 tcacacttcg gacagacctc aagattacgt tccagctcag cgcggtataa aacctgaccg
    45781 cagctatcac acttagtcca caccccttca ggaatgctcg ccttgcgggt gggagtaatg
    45841 ttgcttttaa ttcgttcaat ccagctcatt agggaccttt ctgtctgaac ctggttcgat
    45901 gccagtttta tctttgggga cgcataatgc catttttgcc cccaacagac catgaatgtt
    45961 gcacattaaa acataacagc ccgaaacttt ggataaaaaa gtggtcgaac cgcagagtta
    46021 ctttttattt tgcggcgcgt gccgctgcgc gtttgtggcg aataaattcg aaaacacctg
    46081 gcaatattga aacaaaaata atgccaacaa tcatcagttt aaggttgctc tgaatgaagg
    46141 ggagcgtgcc gaagaaatag cctgcgtagg taaaaagcag tacccacaac agtgcgccaa
    46201 tcacgttata agcggcgaaa tgacggtacg acatgtggcc cattcccgca acaaacgggg
    46261 cgaacgttct gacgatcggc acaaaacggg cgagaataat cgttttgccg ccatgtttct
    46321 cataaaactg atgggttttg tcgagataac tgcgacggaa aattttcgaa ttgggattac
    46381 tgaataactt ttcaccgaac aaccgcccga tagtgtaatt gaccgcgtca cccacaatcg
    46441 cggcgatcag catcagcacc accatcatat ggacgttgag atcgttggtc tccagtgatg
    46501 ccaacgctcc ggcaacaaac agcaatgaat cacccggtaa aaacggcgtt accaccagac
    46561 cggtttcaca gaacaaaatt aagaacaaaa tggcataaac ccatacgccg tactccgcga
    46621 ccagctctgc cagatgcacg tcaatgtgca ggataaaatc gatgaggaaa taaatcaggt
    46681 ccatatttgc ctatgccttg tactcgtcat ctatcaggct gtaactacgt tggctacagc
    46741 ctgaaatgtt ccgagcatta ttctcgttag tccgccagaa atagcgggcc cattggcggt
    46801 tttggaagat catatcggtc agggtaatcc accgcgacca gatatagccc ttccgctttt
    46861 gccgttgctg ccgccagcgt tctgtccttt gccgccagca attctgctat ccagctctcc
    46921 ggctggttgt gggcaccgac ttccatcagg ctgccgacaa tattcctgac catatgatgt
    46981 acaaaggcat tcgctttgat atctaccacc acataaggac cgtgacgcgt gacattaata
    47041 tgcataacgt tgcgccacgg ggtccgggac tggcactgca ccgcacggaa cgaggtgaaa
    47101 tcattctcgc ccagcaagca ttgcgcagcc cgatgcatcc gttcagcgtc cagcggttcg
    47161 taaaaatggg ttaccccttt actcaatacc gccgggcgca gccgatgatt gtagatgatg
    47221 tagcgataac ggcgagccgt ggcgctaaat cgggcatgaa aatcatcagg tacagcttta
    47281 acccaacgca cggcgatgtc accaggtaaa ttcgcattta cgcctaacgt ccacgcagcg
    47341 tctttgcgct gcgcggtcgt ttcgaaatgc accacctgcc cggtaccgtg tacccctgcg
    47401 tcagtacgcc cggcgcagaa gacggtgatg ggttcgttcg ccacctggga gagcgccttt
    47461 tccagcttct cctgcacact gcggacttca ttctgccgtt gccagccgta atacctactg
    47521 ccgtcgtact caatgcccag cgcaatttta taaactggcg gttgttgctg gtcggacatt
    47581 agtacagata ctcctgcacc agtttctcgg cgatttttac tgccatcagc gcgccgccaa
    47641 agcgaacgtt atcggccacc gaccagaact ggacttgctc cggcataccg tagtcattac
    47701 gtacgcagcc aacagaaaga tgcggactac cagaagcatc tcctacctga gtcgggaatt
    47761 cgttctcttc agagagcaca atatcttcgc cctgagcaaa cgcatcacgc gcttcttctg
    47821 ccgccagcgg acgcagtgct tcaaagttga ccatctgggc atgaccgtag aataccggtg
    47881 cctggacgac gcttgccgaa atcatcaccc cttcgtcctg caggattttg cgtacttcgt
    47941 caacgatacg acgttcttca cgcacgctac cttcgctatc cggcagtaac ggcagcatgt
    48001 tgaacgccag ctgacgcccg aagaaatctt cttcgtcaat cggaatgcca ttgagtaatt
    48061 tagcactctg ccccgctaac gcatcgaccg cttttttacc ctgggcggag gctgaaatca
    48121 ggctggtaac gctgatacgt gataaaccgc cctgatcaat tagcggtttc agtgccgcca
    48181 gcagctggct ggtcagactg tctggtacgg cgatgacatt ccggttgcgg taatcggtca
    48241 gtacaaacgg gtttacttcc ggcaccacca gcggtacgtc gggttcgaga gcaaacaatc
    48301 cgctgctgtc gatcaccaga caacctgagt tggttgcttc ttcaacccag gtagcggtag
    48361 cttctttgcc tgcgacaaaa aacgccagct gcgcctgcgt ccagtcgaat tcagcggcat
    48421 cctgcacggt gattgtctta ccaccaaagc gcagttgttc tcctgcgctt tcgttacgtg
    48481 ccagtgcata aatttcccca accgggaact gacgttcagc cagcgtttca agcagggctt
    48541 cgcccacagc gccagttgcg cccaggacgg caatgttcca gccttcagac atggtggttt
    48601 actccagaaa tagcaaagct ccctgccaat gttacagcag agagcatgaa gaagagatta
    48661 acgtgccgga tgatgaacgg cgttaaaacc cagtttacac agcaatgatg ccgcactggc
    48721 gtcatcacaa atcacataca gagatgacca ttcacggcgc tcaagatagt ttttgcgcag
    48781 tttatcgaac tcacccggta tcccggcgac tttacgcagc ggtgcgtcat cgcggcgcac
    48841 atcatacacc aaatgcacca gccttttcag cgtcggttga tcgagcgggc catgcagcgt
    48901 aatgcgacca aactctggcg cgggcagtaa tgtatccagc gcaacgtgct gttcatgccc
    48961 aataaacttg ctataagctt caaacacttg cgtagtaccg cgtgctttac cttccagggt
    49021 atagcctgcg atatgcggcg tgccgatatc cacttttttc agcaattcca cgttaagttc
    49081 cggttcgcct tcccatacat ccagcactac gcttaacttc tggccttcgc tcaggcaggt
    49141 cagcagggcg gtattatcga cgactgcgcc acggcaggcg ttaatcagga tcgctcccgg
    49201 tttcaggctg cggatcagtt tttcatccgc cagatgcagc gttttgtacg gaccatcttt
    49261 aaagagtggc gtatggaaag tcagaatatc cgcatgctgg actaactcat ccagcgagcg
    49321 gaaatcaccc tcatccccac ggtcggcgcg gggcggatcg caaagtaatg ttttgatccc
    49381 taacgcttcc agtcgcgcct gtaaacgacg accaacgtta ccaacgccca cgatccccac
    49441 ggtacgctcg tgcagtgaaa atccatcgcg ttcggcaagc atcagcaggg aggagaaaac
    49501 atattccact accgcaatcg cattacagcc aggtgcagcg gaaaaaccaa ttcccgcctg
    49561 ctttaaccat gcttcatcaa catggtcggt ccccgcggtg gcagtgccaa caaatttaat
    49621 gggttttcct gccagcaaag attcattcac tttcgtgacc gaacgcacca taagcgcatc
    49681 cgcgtcggcc agttgagcaa cgggaattgg acgcccggga accgcggtca cctcacccaa
    49741 acggctaaat aagtcgcggg cataaggcat attttcatca acaaggattt tcacgtttgt
    49801 gttacctgta tgagacgaga gttaaccaga caagtgtgcc ataatctcgc ggccaggcat
    49861 acttgcgaag atttcaggta taaggatacg taatgataca acctatttcc ggccctcctc
    49921 ctgggcaacc accaggtcag ggagataacc tgccgtctgg cgcgggcaat cagcctttat
    49981 ccagtcagca acgtacttcg ctggaaagct taatgacgaa agtgacctca ctgacgcaac
    50041 aacaaagagc agaactgtgg gcgggtatca ggcacgatat tggtctgtcg ggagattcac
    50101 cgctgctttc gcgtcacttc cctgccgctg agcataatct ggcgcaacgt ctgttggccg
    50161 cgcaaaaaag ccattctgcc cgccagcttt tagcgcaatt aggggagtat ttacgtctgg
    50221 ggaataatcg tcaggcggtc acggattata tccgtcataa ctttggtcag acgccgctga
    50281 atcagctctc accggagcaa ttaaaaacca ttctcaccct gttgcaggaa gggaagatgg
    50341 ttattccgca accacagcag cgcgaggcga ccgaccgtcc tttattaccg gcggagcaca
    50401 atgcgctcaa acagctggtg accaaacttg cggcagcaac gggggaaccc agcaaacaga
    50461 tctggcaatc gatgctggaa ctttccgggg tgaaagatgg cgagttaatt ccagcgaaac
    50521 tgtttaacca tctggtgacc tggctacagg cgcgtcagac gctaagccag caaaatacgc
    50581 caacactgga atcactacag atggcgctaa aacaaccttt agatgccagt gaactggcgg
    50641 cgttatcggc atatatccag caaaaatatg gcctttctgc gcaatcatcg ctttcttctg
    50701 cccaggccga ggatattctt aaccagcttt atcaacggcg ggttaaaggg attgagccgc
    50761 gtgatatgca accgctgctt aatccttttc caccgatgat ggacacgttg caaaatatgg
    50821 caacgcgtcc cgcgctgtgg atactgttag tcgcgattat cctgattctg gtctggctgg
    50881 ttcgttaacc ccgacgaaac gacagtatcg tgacaataat tcccagcacc gcagagatcg
    50941 ccccggcaag aaataccgaa gagtaaccaa acgtggtcgc cagcattccc gcaagcggcc
    51001 cggagacgcc gagggcgata tcctgaaacg cggcgtaacc gcccagtgcg gtgccgcgaa
    51061 cgtgtgaggg gacgcgttta accacctcca cgcccagcgc aggaaagata agcgaacatc
    51121 cggctccagt taatgccgcg cccgctaatg cgacccacgc ccctggggct tgccagagca
    51181 gcaacaagcc caccgtttct acaagcagag agacgatcgc cactttcacg ccgccaaaac
    51241 ggtccggcat ccagccaaac atgacgcgca tcacgacaaa tgcgccgcca aacgcggtaa
    51301 gagtaaagcc cgccatcgcc catcctttgc tggcaaagta gagcgaaacg aaagtcccga
    51361 taaccgcaaa accaacgcct tgtagtgcca gacctaaccc tggtttccag ataagcccga
    51421 caacgctcca cagcgatgga cgttcccccg ccagggccgg tactttgcgc actgtgccgt
    51481 tacaggccca cgccagtaag ggtaatgcca ttgtggtgag cgccagtgcg gcaaaaccgt
    51541 aatggctatg aatcaacagg ccaagcggcg caccaacagc gagggcaccg taaatagcca
    51601 ttccgttcca tgacatcact ttgccggaat gttttggccc aacgatgcct aatccccagg
    51661 tcagagcgcc cgtcagtaac tggctttcac caaagccaag aatcaaacgc ccgacgacca
    51721 acagagcaaa tttgaacggt gcggggacgg gcaaaatcgc cgccagcagt aacgcgcccc
    51781 ccgccagacc acaagctaac atcccctgaa gcgccgaacg ttttgcgcca tattgatcgg
    51841 ccagtcgccc ggcgtaacca cgcgtcagca ccgtagccag aaactgaatc ccgacggcaa
    51901 tgccgaccat agtgttgcca tagcccagtt catgatgaac aaacaacggg ataaccggca
    51961 acggtaaacc tacggtcatg taggtgagaa aaaccgcaaa agcgatgcga aagagcgaaa
    52021 aattggcaga agatcgtgtt tcggtttggc ttacagcagt catgcattac tccagaatgc
    52081 agcgcaaggc gaggagtatc cccgtctcat ctctctggtt tcagggttac ggtgcgttgg
    52141 caggatttaa cgcgtacgtc ttttctgcag gaaatcgaca aagcgggaag tttgcctgga
    52201 actggcggcg attgtcaatg atgtgaaaaa gggaaccatc gggttccctt ttgcgttagt
    52261 gccggaggcc gcattggcgc gtaacgtcgg atgcggcgct ggcgcgtctt atccgaccta
    52321 ctccgaatta atctttcagc ttgcgcatta ccagcgtggc gttagtgccg ccgaagccga
    52381 agctgttaga cataactgtg gtcagttcgc gatcggtcgt ttcggtcacg atgttcagac
    52441 ccgcagcctg ctcgtccagc tcttcaatgt tgatgctcgg ggcgataaaa ccgtgttcca
    52501 gcatcagcag agagtagata gcttcctgta cgccagcagc gcccagtgag tgaccggtca
    52561 ttgctttggt tgcagaaatc gccgggctct tatcgccgaa cacttcacgg atagctgcca
    52621 gttctttcac gtcgccaacc ggagtcgaag taccatgaga gttcaggtag tcgattgggg
    52681 tatcaacacc gtgcatcgcc atcttcatgc agcgcactgc accttcgcca gacggagcaa
    52741 ccatgtctgc gccgtcagag gttgcgccgt agccaacgat ttcagcatag atgtgagcac
    52801 cacgcgccag cgcgtgttcc agctcttcaa ccactaccat accgccaccg ccagcgataa
    52861 cgaaaccgtc acggtgagcg tcgtaagtac gggaggcttt ttccggggtg tcgttgtatt
    52921 tagtagacag cgcacccatc gcgtcgaatt cgcaagccat ttcccagcac agctcttcgc
    52981 cgccgccagc aaacacgatg tcctgtttgc ccagttggat ctgctctact gcgttaccga
    53041 tacagtgtgc ggaagtcgca cacgcggagc tgatggagta gttaacgccg tgaattttaa
    53101 acggcgtggc gaggcaggca gaaacgccgg atgccatcgc tttggtgacc acatacgggc
    53161 caaccgcttt caggccgcgc gggccgcgca ttgcgtcagc gccgaaaacc tggaaacgcg
    53221 gggaaccgcc gccggaacct gcaatcaggc caacgcgcgg gttattctgg taagcttccg
    53281 gggagaggcc agcatctgca attgcctgct ccatagaaag gaatgcataa atggatgcgt
    53341 cgctcataaa gcgcacaact ttgcggtcaa tgaggccagt ggtatccagt tttacgttgc
    53401 cccagacgtg gctacgcatg ccggaatcct tcagctcctg agagaaagtg atccctgaac
    53461 gtccttcacg cagagatgcc aggacttcct gctggttatt accgatgctg gaaacaatgc
    53521 ccaggccagt aatcactgca cgtttcattc aatacctctg taagtcgcac atagagtaag
    53581 tttcgcatgc acaatagcgt acacttgtac gccgaacaag tccgatcagc caattaatag
    53641 agaaatttgc gcagcctggc acacatcgct aagatcgagc caccgcctgt aagacgagta
    53701 acttacgtga aacactacgc catacaacct gccaaccttg aatttaatgc tgagggtaca
    53761 cctgtttccc gagattttga cgatgtctat ttttccaacg ataacggact ggaagagacg
    53821 cgttatgttt ttcttggggg taaccaatta gaggcacgtt ttcctgagca tccacatcct
    53881 ctgtttgtgg tagcagagag cggcttcggc accggattaa acttcctgac gctatggcag
    53941 gcatttgatc agtttcgcga agcgcatcca caggcgcaat tacaacgctt acatttcatt
    54001 agttttgaga aatttcccct cgcccgtgcg gatttagcct tagcgcatca acactggccg
    54061 gaactggctc cgtgggcgga acaacttcag gcgcagtggc caatgccctt gcccggttgc
    54121 catcgtttat tgctcgatga aggccacgtg acgctggatt tatggtttgg cgatataaat
    54181 gaactgacca gccaactgga tgattcgcta aatcaaaaag tagatgcctg gtttctggac
    54241 ggctttgcgc cagcgaaaaa cccggatatg tggacgcaaa atctgtttaa ggccatggca
    54301 aggttagcgc gtccgggcgg cacgctggcg acctttacgt ctgccggttt tgtccgccgc
    54361 ggtttgcagg aagccggatt tagcatgcaa aagcgcaaag ggtttgggcg caaacgggaa
    54421 atgctttgtg gggtgatgga acagacatta cccctcccct gctccacgcc gtggtttaac
    54481 cgtacgggca gcaacaaaca ggaagcggca attattggag gcggtattgc cagcgcgttg
    54541 ttgtcactgg cgctattgcg tcgcggctgg caagtaacgc tttattgcgc ggatgaggct
    54601 cccgcactgg gtgcttccgg caatcgccag ggggcgctgt atccgctttt aagtaaacac
    54661 gatgaagcgc taaaccgttt tttctctaat gcgtttactt ttgctcgtcg gttttacgac
    54721 caattacccg ttaaatttga tcatgactgg tgcggcgtca cgcagttagg ctgggatgag
    54781 aaaagccagc ataaaatcgc acagatgttg tcgatggatt tacccgcaga actggctgta
    54841 gccgttgagg caaatgcggt tgaacaaatt acgggcgttg cgacaaattg cagcggtatt
    54901 acttatccgc aaggtggctg gctgtgtccg gcagaactaa cccgtaatgt gctggaactg
    54961 gcgcaacagc agggtttgca gattcattat caatatcagt tacaggattt atcccgtaag
    55021 gatgattgtt ggttgttgaa ttttgcaggt gatcagcaag cgacgcatag tgtggtggta
    55081 ctggcgaacg ggcatcaaat cagccgtttc agccaaacgt catctctgcc agtatattcg
    55141 gtggccggtc aagtcagcca tattccgaca acgccggagc tggcgaaact gaagcaagtg
    55201 ctgtgctatg acggttatct cacgccgcaa aatccggcga atcagcacca ttgtattggt
    55261 gccagttatc accgtggtag cgaagagacg gcatacagtg aggaagatca actgcagaat
    55321 cgccagcggt tgattgattg tttcccacac gcgcaatggg caaaagaggt tgatgtcagt
    55381 ggtaaagagg cgcgctgcgg tgtgcgttgt gctactcgcg atcatctgcc aatggtaggc
    55441 aacgtgcctg attatgacgc tacactcgtg gaatatgcgt cgctggcgga gaagaaagat
    55501 gaagcggtaa gcgcgccggt ttatgacgat ctctttatgt ttgctgcttt aggttcgcgc
    55561 ggtttgtgtt ctgccccgct gtgtgccgag attctggcgg cacagatgag tgaagaaccg
    55621 attccgatgg atgccagcac gctggcggcg ttaaacccga atcggttatg ggtgcggaag
    55681 ttgttgaagg gtaaagcggt taaggcgggg taatctgctc tggcattgtt tgtcggatgc
    55741 ggcgtaaacg ccttatccga cctacgcgtt acctgtaggc ctgataagac gcgataagcg
    55801 tcgcatcagg catggtgctc cagacgtcac aacttactgc tgtgaggcct gctgaaacag
    55861 gtgttcccac atatcggtta ccagcgcctg gtcacgcggt gacaattcac ccgcgccaat
    55921 ggctttttcc agactctggc taacggttgt atgtaccgcc tgagcggagt ggtcatcacc
    55981 actttccagt tctgcgatgg ctaatgtcag gtggccacgc aaatacccac tggcaaacaa
    56041 ctcatcatca ctggcatggt caaccatacc gtcgattaat gccagaatgc gtgattcaaa
    56101 ctccgcgatc atcttctttc ctcattgtaa aacgtggcgc tggcttcagt tgagcgcttc
    56161 cggccacggg aattgttccg ccgtaagttc cggcgtgtga taataatttt gtagtgcttt
    56221 aatgaagcgt gccggacgtt cgggaatgcc gtttgccaga taatccatca cctgcgcatg
    56281 tacccgccgt tgaaacacca cgcggtccgg ttcgaaatcc ccttccagat tgtcgcagct
    56341 aacattaaac ggatatcccg ccgccacgca gaacaaccag tccagcgcct gcggcttcac
    56401 ttcaacatct tcaaactgac tttgcgtctg ggcatcgcgt ccgtccgggc aataccagta
    56461 accgaaatca accagctcac ggcgcgcttt cccggcaata caccagtgcg aaatctcgtg
    56521 gatggcgctg gcataaaagc catgagcgaa gacgattctg ttatacggta cttccgcatc
    56581 agcaggaaga tagatcggtt cgtcgtctcc tttaatcaga cgggtattaa attcatcggc
    56641 aaagcagcta ttaaaaatct caatcaactg ctcatagtgg tgtgtactgt tcattagctc
    56701 atccccaacc agtggaggat ctcctgtcca tggctatcgt aaagtaattt ggcactcatc
    56761 accgccgaga cgataacgat catcgggcgg atcagctttt gtcctttgct caacaccagt
    56821 cttgaaccca tgcgcgcgcc gaggaactgc cccaccagca tcacaaagcc cgtcgcccaa
    56881 atcactttgc cgccgagaat aaacagcagc agaccgccaa tgtttgacgt tgcgttaagt
    56941 aatttggcgt gagcagtggc tttggcgagg ttgaatccgc acagcgtaac gaaggccagc
    57001 gcgtaaaacg atccggcagc cgggccaaag aatccatcgt aaaaaccgac gcagcccccc
    57061 cccaccagcg caaatggcag tccatacatc cgacgctggc gatcttcttc acccagcttt
    57121 ggcatcagca agaaataaag accgatacaa atcaccagaa tgggcaaaat ctgccgtaag
    57181 acatcagcct gaacatactg caccagcaat gcgccactca ttgagccgac aaaagtcatg
    57241 gcgatattga gtttttgatc gcttaaacta accactttac ggcgaataaa gtagatagtg
    57301 gcggaaatag agccaccgca ggcttgcagt ttattggttg ccagcgcgtt agcgggagac
    57361 atccccgccg ccatcaatgc cggaatggtg agtaacccac caccaccggc aatcgaatcg
    57421 ataaatcccg ccagcatggc gacaaaaaag agaactccca gcaacagcgg ggaaaccata
    57481 aacaggctat taaacgtttc cattagatca cgtgctcatc cagtagcgcc tggcaggaag
    57541 gcggcaacgg aggcggtgtc ttcttctcag gctttgttgt tcccggtttt ggtggttcaa
    57601 accagctttg cagttctgcc ccgcaaccat cgcctggtgg cggtaaaggt tgatcttcac
    57661 actccagact atcggcagga caacgtaatc gtacatgcat atgcgcgcga tgctggaacc
    57721 agggtcgcac tttacgtaac cagtcgcgat cggtgcccgc atcaaggcaa agttgttgtt
    57781 taatcgccgg attaacaaaa atgcgcgtga cgtctttgtc ctgggcggcg agtttgatca
    57841 agctgaaaat ttctggcttc catagcgcgg ggacaacgtg tttaccgtcg cgggatacta
    57901 agtccagtgc ttgcgggcgc aagagctgcg cggaggtcca gcgtgttttt ggcagttgca
    57961 gggagatatc gacatccagc ccggtctggt ggctggcgtg accgccgttg aaacgaccac
    58021 cagcgggcat ccccatatcg ccaatcagca ccgtacccat gcccagattg ctcacctggc
    58081 gactcagacg ctggataaac atcaccagat ccgggtgccc gaaatagcga cgctgatcgg
    58141 tacgcatgac ctgataatgc tcggactgta tcggcagcgt gtcagcgccg acgatacaac
    58201 cattggaaaa gctgcctatc gattgtgcgc tacccggcac aggttgggtt attttttgcc
    58261 acggcgtcgc tgccaggctg gcgctactgg caagcagggc cagcagcgca atcgcggttt
    58321 tgttcatttt ttaccagcgt ggaatatcag tcttcacatc ggcattttgc gcccgttgcc
    58381 gtaacaggtg atccatcaaa acgatcgcca gcatcgcttc tgcgatcggc accgcacgga
    58441 tcccgacaca aggatcgtga cggcctttgg tgatcatctc aacttcttcg ccaaagcggt
    58501 taatagtgcg ccccggcaca gtaatgcttg aggtcggttt cagcgccata tgggcaatga
    58561 tttgctgccc gctgctgata ccaccaagaa tgccgcctgc atggttgctc tggaaaccgt
    58621 ctttggtgat ttcgtcacgg ttctggctgc cacgcagcgc taccacgtca aaaccatcac
    58681 caatttccac gcctttcacc gcgttgatgc tcatcagcgc atgggcgatg tcggcatcca
    58741 ggcggtcaaa gaccggctcg ccaagtccgg cggggacgcc actggcaaca acggtgactt
    58801 tcgcgccgat ggagtcgccc tcttttttca gcgcgcgcat cagttcatct aacgcgtcga
    58861 ttttgtccgg gtccgggcag aaaaacggat tttgctcgac ctgcgaccag tctttgattt
    58921 ccagcggaat gtcacccatc tgggtcaggc agccgcggat ttcaatacca aatttctcgg
    58981 cgagatattt tttggcaatc gcccctgccg ccacgcgcat ggcggtttca cgggcggaag
    59041 agcgcccgcc gccgcgataa tcgcgcaggc cgtatttttg ttcgtaagtg taatcggcat
    59101 gacctggacg gaaaacgtcc ttaatcgcac tataatcctg agaacgctgg tcggtatttt
    59161 cgatcaacaa accgatgctg gtgccggtag taacaccttc aaaaacaccg gagagaattt
    59221 tgacctgatc cggctcacga cgctgggtgg tatagcgcga tgtcccaggg cgacgacggt
    59281 cgaggtcatg ttgcaggtcc gcttccgtca gcggaatgcc tggcggaaca ccatcgacga
    59341 tgcagccgag cgccagcccg tgcgattcgc cgaaggtggt tacgcgaaag agttgtccaa
    59401 ttgtgtttcc agtcatcacg gctccgttat tgttgtgttt gcgtgtttac ttaatcttta
    59461 taaatcgcga aatgttcacg ggccgccagc agctgctctt tggtgagcat aaacacacca
    59521 tcgccgccgt tatcaaactc cagccaggtg aacggaacat ccggatattg ttccataaga
    59581 tgtaccatgc tgttgccgac ttcacaaatc agcacgccat catcagcgag gtaatctgcc
    59641 gcgtttccga gaatgcgacg cgtcagtttc aggccgtcgg tgccagatgc caggcccagt
    59701 tccggctcgt ggcggtattc gtttggcagg tcggacatat cttctgcatc gacgtacggc
    59761 gggttggtga cgatcaggtc gtactgcact ttcggcaagt cacggaacaa gtcggaacga
    59821 atcggaatga cgttgtggat caaaccgtgt tcttcgatgt tctgttcagc aaccgtcagc
    59881 gcgtctggag agatgtccac cgcgtcgact tccgcttccg ggaaggcata agcacaggca
    59941 atggcgatgc agccgctacc ggtacacata tctaaaatat gctgcggttg cttgctgatg
    60001 agtccggcaa atttattgtt gatcagttca ccaatcggcg agcgcggcac cagcacgcgt
    60061 tcatcgacgt aaaattcatg gccgcagaac caggctttgt tggtcaggta agccaccgga
    60121 atgcgctcgt tgacgcggcg gatcacgcgc tcaacaatac ggtgtttttc gctggaggtc
    60181 agacgcgcgg tgcgcatgtc ttccggaata tccagcggca ggtagagcga aggcaacacc
    60241 agttgcacgg cttcatccca cgggttatcg gtgccgtggc cgtaccagat attcgccgcg
    60301 ctgaagcggc tcaccgacca gcgcaacatg tcctgaatgg tttgcagctc atttactgct
    60361 tcatcaacga aaattttatc cacgtattcc tccagggcat gatcgcaata atttcggcgg
    60421 ctagtttgcc atgaagatga cgataaatca gcattcacgc gcggtgagtg aggaaaaata
    60481 cgtttaaaac gatcgattgt gctacgagtc gggtaaactg taggaaaatt agaaatagag
    60541 acagttaaat gaaaaagaaa acaacactta gcgaggagga ccaggcgctg tttcgccagt
    60601 tgatggcggg gactcgcaag attaagcagg acacgattgt ccatcgacca caacgcaaga
    60661 aaatcagcga agtaccggtc aagcggttga ttcaggagca ggccgatgcc agccattatt
    60721 tctctgacga gtttcaacca ttattaaata ctgaaggtcc ggtgaaatat gttcgcccgg
    60781 atgtcagcca ttttgaagcg aagaaactgc ggcgcggtga ttattccccg gagttgtttc
    60841 ttgatttgca cggtctgacg cagctacaag ccaaacagga actgggggcg ttgattgccg
    60901 cctgccgccg tgaacatgtg ttttgcgcct gtgtgatgca tggtcatggt aagcatattt
    60961 tgaagcagca aacgccactg tggctggcgc aacatcccca tgtaatggca tttcaccagg
    61021 caccaaaaga gtatggtggt gatgcggcat tgctggtgtt gatagaagta gatgagtggc
    61081 tgccgccgaa attgccctga atatttaaag gtggacgagt ctgtccattt agccttgaat
    61141 actggcgtct caaccctggc gataatgaac gtaataatcg ggcattaatt gaaaaacgta
    61201 agcagctttc cgacagcctt ctgtgggcta atggcattgc cccttcagag gggcttgagg
    61261 atgacgacct gctgcaagcg cttatcgacg aattactcac cctcgtcgac ggttatctgg
    61321 cacctttcgc tcccgtgatg gcgaatattc taaaaaactg gatgaccgca tgacataacg
    61381 gacaacgggg ccagatggcc ccgttgtgtg gtgtatacgc tacagctcgt tgagcgtgta
    61441 ctggaagcgg caggtggaat caatcggctg gcggctgctg gtatcgacac gcaccaccat
    61501 gtcgtgcagt tgcttgtcag tgatgggatg cctgtttggc gccaccgtaa cattagcatt
    61561 atcacagcct gatgatgaaa acgtgatcat ttgcgcaacc cagcgacagt aagcgccctc
    61621 ttcattcatt tgcgagccat cacatagttt gccgtctttc atcagatagg ttgaaagcgt
    61681 tttttcaatc acaccccccg tcgattgcaa agacagtgtt ttcgttttat tgatatagtt
    61741 ttcatcccac gaaacatcat acggcgtaac tgacacggtt gggcacacgg acacggttgc
    61801 atttggtcca taatagatat ctttatcaac agaaccgacc acaacctggc tggtggctct
    61861 gatactcact aaccacgggc agcctcccac atcaccatcg acggtaatat ccagaggcag
    61921 cttgagacat tcccagcggc tgtctttggc ttccagtgcc tccatgcccg gacaaaatga
    61981 attatttaca attgtcccat cttttaccgt aatcgtcggc gcaggagaac tgcccgttcc
    62041 tgtccatgtg cgattgggta agttgccaag cgtcataaca taattacttt gataataggt
    62101 accgtagtaa acactcgaaa cctcagcaac atcaagatac agttcgacct gactattcca
    62161 gccaaacgta gcatagtaga tatacacatt acgaaatgtc tgccccatcc cggcccgact
    62221 accgagcata aaaaccatca ggatcaaaag acgaaacagc aagttagttt ttttcatctt
    62281 atatgtactc aaacgttgcg gttaaggagg cggtaaactc acccatcgtc acttcccgcc
    62341 cgcttttcgc ctggagccag gcattaaaat tcagattgtt attgccgtta ctcagcacaa
    62401 aggtcgcgcc gctggtattg ttaatcggca ccggtgttcc atcgtttttt tccatgccaa
    62461 ttccgacacc ttgcgccgcc gaagatacat ccagcgccag aaaaccgggc tgttcactgt
    62521 cttctgtacc ggtcagcgtc acccgaacct catactgagc tggccctttg caatctttga
    62581 gctgaaacac cactggcacc cgcgccgact gcccggtcac catcaggtct ttacggctga
    62641 tggtgggaaa atgtacctcc gccagcgttt cgccctggac caccagcgtg caggatttac
    62701 tgagtaaatt gccgtagaaa tgcaggttgc cagccgcctg aacatggggc gtcagcagca
    62761 ccgtcgccag tacagccgcg cagagagata tccgttttag gttcattggt aatccacctg
    62821 taatgttgcg taggcagaaa aggttccgtc gctgagcgcc gcatcattgg ctttcaccgg
    62881 cacagcttgc agcgttggcg gcgttgacgg gtcaattttc aacggtgttc ccagcttaaa
    62941 cggctggccg ttttgctgta gctcaatccc caggccgcta acgtccgttt caatggcgga
    63001 atcatcataa ggggtttggc tgcccgtcca ggtcaggctc atctcccagg cgtcgtcgcg
    63061 cacatcgggg tcacatgtga tggtgtaagg cacatcctga cggaaattat cgccgttaat
    63121 gttgtcgata atgacgttgc gaaactcaac ttcgatcgtc ttaccatcgc tgatagtaca
    63181 ggcgggtggc gaaaccagcg tgccgtgaaa agtaacgtcg ttaatcgcgg cctgcgccag
    63241 gccagcaaaa ccgtaaatta agcaccatgc gagtcgtttc attggtagtc caccaccatg
    63301 gtcatggtcg cattaaactc cgatgccatc agttccacac cgctctgctt cacaggaacg
    63361 gcttcgaagt ctggctgagt attgatgttg aacggcagcc agctactgtt acccacggcc
    63421 accagcgaat ggtcagcggc attctgaata cgaatgccaa aaccgctgat tccagtactc
    63481 agcaccgttt cgccgttgat ggtcgtggtg gtggcctgaa gctgcatttg caggtcgtcg
    63541 ctgacactgt ttgagcagtt cagggtgtag cccactgcct gacggtagtt ggcaccatca
    63601 atgcttttaa taatcacatt gccgaactcc accgcgcttc cctttaccga acagggcggc
    63661 ggtccatcca ccagcacgcg caacgtcaga ttgacgattt tgccgcccgc cattgccggg
    63721 aaaacggtca cgccacaggc cagcaatagc gcggcagatc cggtcagaaa gaacgctttc
    63781 atgttgcttc tcccgttatc aacctttgct gctttgatct tcatccacct tgcaggtgtt
    63841 cccttcacaa cggaagatca gcggcatgcg cgcgccgtag tcgttgatat aggtcagcac
    63901 cggggcgctc gccatttttg cctttaacgg ttgtgacgtt ttcggtggca tcaccagcgg
    63961 tttaaacccg gcggcagtca gccctttctt ctgtgtgctg gcatcgctga tgatgacgtg
    64021 gtacggcgtc gggttattga cggtatagtc ctggccgctg cgcgtgagtg tgaccttaaa
    64081 ctgccacggt tttctcatgt caatcttctc cagcgccttt ggtcgccaga acagcttgat
    64141 gcgcgtttgc agcgcaattt gcagagtgtt ggctttcccg gactttggcg ggatctcacg
    64201 cacgttgtag tagaacaggc tttctcggtc ggacggcagc gtgtgaatgt ccggcagcgc
    64261 ctgaatcttc acctggccgt tcatcatagc gtcaatacgc tgcaccggcg gcagtactgc
    64321 cagcggcgag atgattttgt tgcccttctc gtcttccagc cagctttgcg ccaggtaagg
    64381 cagcttttcg ttgttgttac gcaacgtcac gctgatcgat ttatcgcttt cgttaaacac
    64441 cagacgggtg cgatccgggg tgaccgatgc atgcgccgtc aggctggcgg tggtaagcat
    64501 tgccatcagc gctgtgctga cgagcattgg actggataat ttataagaca ttgatcgttc
    64561 cttaatcaga ttattgagtt gctgaaattg acgtcggcgc ttccgttggt gtgacttgcc
    64621 gcgtctggtc ctgaataaca ggcttaattt ctggcgctga agaatcaggg gctgccgttc
    64681 ccttttgttc acacggcaac aacaggccgc tgaacagatc ggcaggtagc ggcttcggca
    64741 atacgatctc gcattgtgcg ctgccttccc agaacaccat catatgctcg cccgcattga
    64801 cccccgccag atagacattg ccttcatcat ccacaatgcc aacctgctgc tgctcgtcgt
    64861 ttttcacttc cgcgccaaac ggcgggtagc taccgtcacg caaccgcagt accgccatcg
    64921 ctttttgccc gctgatcact ttgaatttac gatagccgat tgcgccttcc gtcagagtcg
    64981 cctgcaccac cgactgggtg gcttccgcat cttccggcaa gttgtttaaa tcgatatacg
    65041 cctgattgcg atagtagttg ttaatatcgg cgaccaccgc cttgccaaac atattggtgt
    65101 acaccggcgc gccgttgctt tcgaccggca cgttggcaat cccgtcggcg tcaatcagca
    65161 ggcgcgtgcc gcccatgttc tgggtacggt gcagcgcccc accatgcgcg gtcagcgttg
    65221 ccccgccctg taaggcgatc cccgccgaac ggtattcccc ttcatggtag ttggcgctga
    65281 gatcaacctt cgccagcgaa ccgtcgtggc tcagataacc atccacgctg ccgtgctgct
    65341 cgctggtgcc aacgttaacc tggtagtgcg ttgcgtcatc gacgcgctta aagtaaccga
    65401 cctggctgct gtccgacccg ctgccgtagg aaccgttgta ggtcacggtg ctgctgtcgc
    65461 tccacggaat gctcatactg aggtacatcc ccttatccgc attatcgtca tattcgtagc
    65521 gatagccggt caccgagatg ctcatgttgc gaatgctccc catattaaaa tagtgggaaa
    65581 acatcaagtt atagtttgtc tgttccgggc gatcccagta agtacgatgg gagtagttga
    65641 gatagatcga aacacccgca gcggcaaagt tctggttata ggtgatcgta tacatctctt
    65701 tgtcgttacc ggtccgcgcc atgtctgact ggttcgcgtc cagatactcg ctcatggtca
    65761 tgaagttctt ttcagaaaaa cggtagcctg caaaggtgac gcggctgttg agttcgtcaa
    65821 agtctttggc atagctcacg cgaaaggagt tgccgtccag ttttcctttg ccgtatgcgc
    65881 tgtcatgatc caggttgacg tgcgagtgag tcacatcgaa cgccagcgcg ccaaactgtg
    65941 cgaggtcgcg ccccaccccc atcgccgccg actgatagtg tttatctgcc agcgcaccac
    66001 cgtagagcga ccagccatct gccacccccc atgacgcttc tccgccggag aaaaagccgc
    66061 cttcggtctt gtggttccag tcttccggac gccccatcat cactttgtaa cgcacctgcc
    66121 cctggcgcgt gaggaatggc atagatgcgg tggtaacgtc atattcctgc acctgaccat
    66181 tctgttcttc aacgcggacg tgcagtgtgc cggagacgga gtcgccgata tcctgaatgc
    66241 ggaatggccc ggcgggaacc tgggtttcgt aaagtacccg gcccatctgg ctaatggtga
    66301 cttttgcact gctgtgcgcc acgccggaaa catccggtgc atagccgcgc aggttgggcg
    66361 gcagcatctg gtcatcggtg ctgacgctgc tgccgatata gttaaagccg tcgaaaatat
    66421 cggaattgag ataatctttc cccagcgaca gcttcgcttt taaagagggc aaggctcgcc
    66481 aggcataata acgactccag tcccagtgtt tactcgttgt gctgttactg ctgtcatcgt
    66541 cgtcatcgtt gctgcgggtg tgctgataat cactttgcca gtctgcacgg aaacgccatg
    66601 cccccaggtt cgccccaacg gtaccgttgc cgctgatatc atgtgagtcc tcgcccccct
    66661 gctcctggtg gcgagtttgc gcattgaggc tgtagtcggc aatcaggcca ggaataccgt
    66721 cgtcccaacg tgagggagga tcccagtcgc tgctggtata ttcgaggtaa gcctgtggca
    66781 ctgtcagcag caacgccgac tggcttaaat cattctcgac ttccatcccg tctaattgac
    66841 ctggtttaag gcattcatcg ttgtgcgtcc actgcaggct ttttgctatc ccttctttca
    66901 gccccagcgc cgccactaac tcaggtgtca ggcaggcata gttttttgtt ggatcgtttt
    66961 cagagacgta ccagttaatg tcgtattcat cagaaagggg ttgtttattt ataaatacac
    67021 gtaaattata tttacccgcg tcgacatacc ctttttttga aaacttaccg agatcaattt
    67081 tcgtatcgcc ctttaactca aggaaacggg gggcaaattg aattgaatca tcagcccatg
    67141 cattgactga cccaccagag attgccagcg caatatagca ggcgattccc cgcagccgaa
    67201 aatttgagtg attaggcata cacatatccg tttgtgaaac agtcgtaaat ctgttgcgtg
    67261 ttgaataacc ccacagcaga acgctgtggg gttgtcacat cagagataag taatctggaa
    67321 tgtggtcagg gtttcgaact ggccgagatc cggcgcatcg gtttcaccca ccagccaggc
    67381 tttaaagttc agcgtttgct tgtctttacc cttgctggtt gaagtatcca tcacgattgg
    67441 ctgaacaatc ttttcgccca ctttataacc tttgccatgg gtatcgccga ttaccaggct
    67501 cacgtggttc atggcaacac cgctgtcaac attgaataac gcatagttat ccgtgttggc
    67561 tggccccgcg tagttagtac cggtgaacgt ggtctccatg tttgtttcag tggtaaacac
    67621 gcaatcctgt aaacggatct ggaaatcttt cggtgtggta gtgccaccgc cggtcagttt
    67681 agccgcaccg atgtcaccca tatcgacttc cagtttgtga tcgtctggaa cgatagagca
    67741 cggggagtct tcaatggtgc cgtagaagcg cgcggtaccg ttgttgcctg ctgcaaaagc
    67801 agaactggaa acaactgcgc ccatgaccat cgccgcagca atggctgttt tggcaaactt
    67861 actcattatt tgtccttaaa tcattaagag ttataatata agcacctgac ataaaattaa
    67921 tatatattat gcccgatatt tat
//
LOCUS       DAZFFE010000010        66830 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFFE010000010 DAZFFE010000000
VERSION     DAZFFE010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.010"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..4362
                     /locus_tag="ACHJ69_000813"
     CDS             <1..4362
                     /locus_tag="ACHJ69_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO5013867.1"
                     /translation="GDEATVNNNGSTTVDGKDSTGTEIAGNNATVTQEGELTVSSGGR
                     GIDITGNNAKVDTKGKMTITGTDSVGVSINGDSATLTNTGDIDVSNSATGFSLVTNEG
                     IISLAGSMKVGDFSTGMALSGDNNSVTLAAKDINVTGQKATGVNISGESNTVDITGNI
                     LVDKDQTADNAVVYFYDPSVGVNISGNSNTVSLDGKLTVIADSELTSRKYMEFDGSQE
                     NISGLTVSGDGNTVNLNGGIQFVGEKNALADGSTIADKRSYFGKTPLVSVDGQSKVYL
                     NGDSTISGSLPLGYANILQLSNKAALEIGSDATFSMQDISVYEHYFTQTPQIIKVDTG
                     SQVVNNGDVDIWNISFAGIWGENSTGINNGNITLSQYDYSSPETSFSEPDHMAFLSSS
                     GGSAVNNGTITAKVMEQHSVLNLGSAAGVADPRVFNNSVSSMMGMEAYGKGTVLNSES
                     GVIDMYGRGNIGMLAVDDSAADNAGKITLDTLWVDQNDTTTLRTDLPSSTAIDYGVGM
                     ATGTNSGGGARSNGVATNQQGGVITVYNAGAAMAAYGASNMVINQGIINLEKNGNYDG
                     GLGANMLVGMAVYNRGTAINDKTGVININVDTGQAFYNDGTGTILNYGEINLLGSPMD
                     SADSHMGAIPENLDLLTALTGSGETDMRTASSGGFVTTKALANYGNETLNSNVAAKAW
                     LYNQDKANLTINGELSIGQGLENSGLLDSDTISAAANVYNRASGSIITDQLSLTGSNS
                     FFNEGNFSGSVAGSSYKQNVVNTGTMAVMADGKSLISGSFLLYNEAGATLSNSSSAVS
                     GGENAIVNVTRTGDSLAQVNRGTITAVNGYSAIKTASTGSNSNGKWIWNTDTGVISGV
                     NPNAPLIDLGRGYNFANAGTINVQGDGAVAISGGTTSYTVQLVNSGTINVGTAQGKAD
                     GTNGTGLIGIKGNGSDTTINNAQSGVINVYADNSWAFGGKTKAIINNGEINLLCDTGC
                     DIYAPGTTGTLNDHNSTTDIIVPAATSTPTQGSVPTVPADSSAQQKLTNYTIGTNSDG
                     TSGMLKANNLVISDNVKVNTGYSAGTADTTVVINDVFKGENISGAENISSSTVMWNAQ
                     GSTDASGNVDVTMTKNAYTDVVTDSSVNNVAQVLDSGYTNNDLYTSLNVGTTAELNSA
                     LKQISGSQATTVFNEARVLSNRFSMLSDAAPEVANGLAFNVVAKGDPRAELGNNTQYD
                     MMALRKSMTLTEYQNLSLEYGIARLEGNGSDTVGDNGVTGGYSQFFGLKHQMAFDNGM
                     SWNNALRYDVHNLDSSRSIAYGDVNKTADANVKQQYLEFRSEGAKTFELREGLNVTPY
                     AGVKLRHTLENGYQERNAGDFNLSMNSGSETAVDSIVGLKLDYAGKEGWSANATLEGG
                     PNLSYVKSQRTASISGAGSQRFNIDDGQSGGGFNSLATMGVKYSSQESALQLDAFHWK
                     EDGISDKGVMLNFKKTF"
     gene            4571..5432
                     /gene="pdxI"
                     /locus_tag="ACHJ69_000814"
                     /pseudo
     CDS             4571..5432
                     /gene="pdxI"
                     /locus_tag="ACHJ69_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
     gene            5731..6336
                     /gene="ynbA"
                     /locus_tag="ACHJ69_000815"
     CDS             5731..6336
                     /gene="ynbA"
                     /locus_tag="ACHJ69_000815"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO5013868.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLVLVAQPILFLLLPIVLFIRMTLNALDGMLARECNQKTRLGAILNETGDVISDIVLY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLFMSAER"
     gene            6336..7232
                     /gene="ynbB"
                     /locus_tag="ACHJ69_000816"
     CDS             6336..7232
                     /gene="ynbB"
                     /locus_tag="ACHJ69_000816"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO5013869.1"
                     /translation="MLEKSLATLFALLILATLINRFLVWRLPERKGGEVTLRIRTWWG
                     IVICFSMVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWGIPLNYLLIGFNC
                     FELFLLFIPLAGFLILATWRVLVGDPSGFLHTVSAIFCGWVMTVFTLSHAAWLLMLPT
                     INIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQVLLAGLLIGISGFCGDVVMSATKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            7248..9005
                     /gene="ynbC"
                     /locus_tag="ACHJ69_000817"
     CDS             7248..9005
                     /gene="ynbC"
                     /locus_tag="ACHJ69_000817"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO5013870.1"
                     /translation="MENSRIPGGHFFTTSDNTALFYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVMVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPGLALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLSTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGV
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRFIDKIYLNSVGWRGIRQRKTHLQ
                     MLIKQAVAHLHAKGLAVRVVDIAAGHGRYVLDALANEPAISEILLRDYSEVNVAQGQE
                     MIAQRGMSGRARFEQGDAFNPAELSTLTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIDPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVHDTGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            9019..10311
                     /gene="ynbD"
                     /locus_tag="ACHJ69_000818"
     CDS             9019..10311
                     /gene="ynbD"
                     /locus_tag="ACHJ69_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO5013871.1"
                     /translation="MLQGAGWLLLLAPFFFFTYGFLNQFTATQDLNNHDIPSQVFGWE
                     TAIPFLPWTIVPYWSLDLLYGFSLFICSSTFEQRRLVHRLILATVMACCGFFLYPLKF
                     SFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVRWRKVCGGW
                     FLLIAISTLTTWQHHFIDVITGLAVGMLIDWIVPVDRRWNYQKPDQRRIKIALPYVVG
                     ACSCIVLMELMIMLQLWWSVWLCWPVLSLFIIGRGYGGLGAITTGKDSQGKLPPAVYW
                     LTLPWRIGMWLSMRWFCLRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTFEFPRGRAT
                     KDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLL
                     CYGHCKTVDEAISYIRARRSRIVLKEEHKAMLKLWENR"
     gene            complement(10365..10970)
                     /gene="azoR"
                     /locus_tag="ACHJ69_000819"
     CDS             complement(10365..10970)
                     /gene="azoR"
                     /locus_tag="ACHJ69_000819"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO5013872.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSTDEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAYDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAE"
     gene            11171..15073
                     /gene="hrpA"
                     /locus_tag="ACHJ69_000820"
     CDS             11171..15073
                     /gene="hrpA"
                     /locus_tag="ACHJ69_000820"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO5013873.1"
                     /translation="MTEQQKLTFTALQQRLDSMMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDHAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPADYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKTRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELIIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAIDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EVRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            15346..16146
                     /gene="ydcF"
                     /locus_tag="ACHJ69_000821"
     CDS             15346..16146
                     /gene="ydcF"
                     /locus_tag="ACHJ69_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO5013874.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLVQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRITGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            16343..17782
                     /gene="aldA"
                     /locus_tag="ACHJ69_000822"
     CDS             16343..17782
                     /gene="aldA"
                     /locus_tag="ACHJ69_000822"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO5013875.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERATEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKSIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(17824..18825)
                     /gene="gap"
                     /locus_tag="ACHJ69_000823"
     CDS             complement(17824..18825)
                     /gene="gap"
                     /locus_tag="ACHJ69_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO5013876.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSIDYTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDDNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKKATNNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            19014..19544
                     /gene="cybB"
                     /locus_tag="ACHJ69_000824"
     CDS             19014..19544
                     /gene="cybB"
                     /locus_tag="ACHJ69_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO5013877.1"
                     /translation="MGNKYSRLQISIHWLVFLLVIVAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPITPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            19789..19962
                     /locus_tag="ACHJ69_000825"
     CDS             19789..19962
                     /locus_tag="ACHJ69_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013878.1"
                     /translation="MKKLALILVMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSIRASKKTCTN"
     gene            19988..20056
                     /locus_tag="ACHJ69_000826"
     CDS             19988..20056
                     /locus_tag="ACHJ69_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013879.1"
                     /translation="MNPLVRCAGKIPALALTWTCRP"
     gene            complement(20034..20135)
                     /locus_tag="ACHJ69_000827"
                     /pseudo
     CDS             complement(20034..20135)
                     /locus_tag="ACHJ69_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
     gene            20146..20577
                     /locus_tag="ACHJ69_000828"
                     /pseudo
     CDS             20146..20577
                     /locus_tag="ACHJ69_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(20615..21538)
                     /gene="ydcI"
                     /locus_tag="ACHJ69_000829"
     CDS             complement(20615..21538)
                     /gene="ydcI"
                     /locus_tag="ACHJ69_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5013880.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMNGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            21755..23098
                     /gene="ydcJ"
                     /locus_tag="ACHJ69_000830"
     CDS             21755..23098
                     /gene="ydcJ"
                     /locus_tag="ACHJ69_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO5013881.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQVHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKVLIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLGWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQACNHGDASREAFEQALGGPVLDEFQLYQEAEERSKRRCGLL"
     gene            23323..24978
                     /gene="opgD"
                     /locus_tag="ACHJ69_000831"
                     /gene_synonym="mdoD"
     CDS             23323..24978
                     /gene="opgD"
                     /locus_tag="ACHJ69_000831"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO5013882.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKNQVIMDVENHLYARKDIKQLGIAP
                     MTSMFNCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            25118..25342
                     /gene="ydcH"
                     /locus_tag="ACHJ69_000832"
     CDS             25118..25342
                     /gene="ydcH"
                     /locus_tag="ACHJ69_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO5013883.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQVSVKEV"
     gene            25405..25944
                     /gene="rimL"
                     /locus_tag="ACHJ69_000833"
     CDS             25405..25944
                     /gene="rimL"
                     /locus_tag="ACHJ69_000833"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO5013884.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(25936..26915)
                     /gene="ydcK"
                     /locus_tag="ACHJ69_000834"
                     /pseudo
     CDS             complement(25936..26915)
                     /gene="ydcK"
                     /locus_tag="ACHJ69_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
     gene            27039..28031
                     /gene="tehA"
                     /locus_tag="ACHJ69_000835"
     CDS             27039..28031
                     /gene="tehA"
                     /locus_tag="ACHJ69_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO5013885.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTGAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALD
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTTLRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            28028..28621
                     /gene="tehB"
                     /locus_tag="ACHJ69_000836"
     CDS             28028..28621
                     /gene="tehB"
                     /locus_tag="ACHJ69_000836"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO5013886.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            28924..29592
                     /gene="ydcL"
                     /locus_tag="ACHJ69_000837"
     CDS             28924..29592
                     /gene="ydcL"
                     /locus_tag="ACHJ69_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO5013887.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(29627..30817)
                     /gene="ydcO"
                     /locus_tag="ACHJ69_000838"
     CDS             complement(29627..30817)
                     /gene="ydcO"
                     /locus_tag="ACHJ69_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO5013888.1"
                     /translation="MTYEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGA
                     TTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGV
                     FIVTNVLIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSM
                     LLVWLATRAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYIPPDFSFAHSL
                     SVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAI
                     TAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQMLAGLAL
                     LSTIGGSLYQALHNERERDAAVVAFLVTASGLTLFGIGSAFWGLIAGGVCYVVLNLIA
                     NRNR"
     gene            30891..31427
                     /gene="sutR"
                     /locus_tag="ACHJ69_000839"
     CDS             30891..31427
                     /gene="sutR"
                     /locus_tag="ACHJ69_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO5013889.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQRQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVFVIDGQLDLCVDGEWQSLNCGEGVRFAADVTHVYR
                     NGGEQTVHFHSLIHYPRS"
     gene            31500..33461
                     /gene="rlhA"
                     /locus_tag="ACHJ69_000840"
     CDS             31500..33461
                     /gene="rlhA"
                     /locus_tag="ACHJ69_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO5013890.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARNVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDDLLKTLPKRKG"
     gene            complement(33553..33738)
                     /locus_tag="ACHJ69_000841"
                     /pseudo
     CDS             complement(33553..33738)
                     /locus_tag="ACHJ69_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
     gene            complement(33990..34520)
                     /locus_tag="ACHJ69_000842"
     CDS             complement(33990..34520)
                     /locus_tag="ACHJ69_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013891.1"
                     /translation="MSQGKEPVIERIGMLTKRYPSRSAAARAWGININTLNSYFKGGQ
                     TQPAPRESILIRISESEGVSLEWLKEGKGELPNDIGIYGNFGCSGDKGDQLTQMLSFL
                     TKDEREQLTNLLARKGVETVLTLLNKENQDLLQLEGRKRLAALMIVDMPDDCVREILE
                     KTETSRQSNPVESKAG"
     gene            34711..34959
                     /locus_tag="ACHJ69_000843"
     CDS             34711..34959
                     /locus_tag="ACHJ69_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5013892.1"
                     /translation="MSRREVSDHDWPPEIIKARLHMAGLSLRSLSLKAGYSRDSLKSV
                     LRTPCRPYQQIIADALGVSPEEIWPSRYQVKSYMRKAS"
     gene            34961..37051
                     /locus_tag="ACHJ69_000844"
     CDS             34961..37051
                     /locus_tag="ACHJ69_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFO5013893.1"
                     /translation="MYVIAKELIGAPGMPATTKGIRQALQRYVQGKSCCSRRRSGSKA
                     TEYSIDCLPEVTQQALRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRKVAEARIAIVSEVLKIAQQPGFSCAKAIRFLVNSLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADVLDKEIVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFSALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPMKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRPDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVTVQQPVADDEPDEEYERNFQRGL
                     QLLEAKLKESDPLA"
     gene            37134..38066
                     /locus_tag="ACHJ69_000845"
     CDS             37134..38066
                     /locus_tag="ACHJ69_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5013894.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFDDL
                     SRLFSRLARTKQLRKAKKADVLAIAMAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            38069..38290
                     /locus_tag="ACHJ69_000846"
     CDS             38069..38290
                     /locus_tag="ACHJ69_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013895.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATEVYSGICRSVGAKQVS"
     gene            38303..38557
                     /locus_tag="ACHJ69_000847"
     CDS             38303..38557
                     /locus_tag="ACHJ69_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013896.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            38559..38840
                     /locus_tag="ACHJ69_000848"
     CDS             38559..38840
                     /locus_tag="ACHJ69_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HFO5013897.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            38837..39109
                     /locus_tag="ACHJ69_000849"
     CDS             38837..39109
                     /locus_tag="ACHJ69_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013898.1"
                     /translation="MSKVVRIIFEYKEDVICKNSDGSTHRGVSLDIRSTGIKKKDNGP
                     AMIFGVVMLAESRNFAEFVAMKASAFMKDRGMASGVINGNEFNQQG"
     gene            39114..39407
                     /locus_tag="ACHJ69_000850"
     CDS             39114..39407
                     /locus_tag="ACHJ69_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013899.1"
                     /translation="MSKVRVIFEFNHVSHDEKLAGNDCVEVHEKIGVDVKTERDTDNR
                     PTSLCDVYASILQYHSPAIIQFLSAEFQASAQSFGADAIIKRHRVHKASGTLQ"
     gene            39419..39949
                     /locus_tag="ACHJ69_000851"
     CDS             39419..39949
                     /locus_tag="ACHJ69_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO5013900.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKQIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGTPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            40095..40628
                     /locus_tag="ACHJ69_000852"
     CDS             40095..40628
                     /locus_tag="ACHJ69_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013901.1"
                     /translation="MIDAKVLEGVKNWLCIYGRLTCGVLAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQESAADDVQWCGFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITEHVLRY
                     LIVDRTAAPAGISAEVA"
     gene            40628..41143
                     /locus_tag="ACHJ69_000853"
     CDS             40628..41143
                     /locus_tag="ACHJ69_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013902.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQKVKDEAKRLEAVLNVACRSVFV
                     SGISGFYFKGLRFTEDKYPFHRDLWRKPTASNGWSCTPRTSRIPKTLRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAILFGPVKWFCVDDVIYLQCGVKPAKQKMTEILSD
                     EFYAAEKRVRG"
     gene            41147..41698
                     /locus_tag="ACHJ69_000854"
     CDS             41147..41698
                     /locus_tag="ACHJ69_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SANT/Myb-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFO5013903.1"
                     /translation="MMKLQPMGLKGRAPAHVRPWTPEEDALLIALYSSTPVKDIAARI
                     KRTVWAVYNRTGVLRSSYPELLKYKHPRFTPDEDKFIRKNARTMTCQQMGEYLGRNKD
                     SVRCRAGMIGAGLTKCGELRPGTRISDDDVRLIRALRDSDYPRRLSFREIGEKFGISE
                     HSAHAVYYRRRTAEDAVLRELTP"
     gene            41695..42027
                     /locus_tag="ACHJ69_000855"
     CDS             41695..42027
                     /locus_tag="ACHJ69_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013904.1"
                     /translation="MITTLFVESDEPLVCAAGMPLCGGTLTGVYFGDLRGYPWHSLDD
                     AFPPDMEAVVLIVQYGDQQELRIGHMGYEGFFIDEETGACLEDEDGQVTHWCHLSALP
                     ELQGIHNG"
     gene            42165..42452
                     /locus_tag="ACHJ69_000856"
     CDS             42165..42452
                     /locus_tag="ACHJ69_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013905.1"
                     /translation="MEKEIKFAPKSIDEELAKIGMLERMRDIIEYAIKENLAAREALL
                     IMEREINLIKDAVSLDNKIAREEYVRRRLGVDGSAILTSEHYAKIFNLFQR"
     gene            complement(42433..42672)
                     /locus_tag="ACHJ69_000857"
     CDS             complement(42433..42672)
                     /locus_tag="ACHJ69_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013906.1"
                     /translation="MSDIKNALDVAAFTMAGNLMVTLFDKGLISREEAIHIINLAQKN
                     STPENAEDIQDGSYINTDWHFDNLRACMGITAEKD"
     gene            42743..43255
                     /locus_tag="ACHJ69_000858"
     CDS             42743..43255
                     /locus_tag="ACHJ69_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFO5013907.1"
                     /translation="MNRASLITLIHVAKRDLQLDHDTYTSALLAATGKSSCRDMSPGE
                     LSRVLDVFKKRGFKVRQKPVNRALKPGTVTAKIRAIWKVMHRQGFISDGGETALNRWV
                     KSQTAAQNDGEGVANWQWLEQQPALASDVLERLKRWHRRKMLAALDMSERAVMSYERV
                     CRLYEKSFPR"
     gene            43326..43748
                     /locus_tag="ACHJ69_000859"
     CDS             43326..43748
                     /locus_tag="ACHJ69_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFO5013908.1"
                     /translation="MAETQMSMFGDSEQLHALIDRLDDIPDDELKKNWPGTLRDLVDV
                     ISAELHRQGIEPAQAGMLARKVAAAQAGYMGGRGYYLPVGESLFTELRNNEIFFRWSQ
                     GEKIETLRRHYQMSETQIYSVIRNQRRLHQERTQPRLF"
     gene            43820..44320
                     /locus_tag="ACHJ69_000860"
     CDS             43820..44320
                     /locus_tag="ACHJ69_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5013909.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENTTYIDP
                     TGTPTICYGHTGPDVKPGMTKTDEECLELLEKDMKWAFAAIDRHVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            44433..44783
                     /locus_tag="ACHJ69_000861"
     CDS             44433..44783
                     /locus_tag="ACHJ69_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013910.1"
                     /translation="MAALGVMSVLAAVVWGMHQKTQRLEDNNHVLVRERDEARLVLAN
                     QQRTLQLISQISKAATNEKQQNMQHSEEQQSIVRRSLATVPAAAVPVPDDVADRVRRA
                     VCEIRPCAAGADPR"
     gene            44767..44985
                     /locus_tag="ACHJ69_000862"
     CDS             44767..44985
                     /locus_tag="ACHJ69_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013911.1"
                     /translation="MPIPAEWLADCLVPPAPQPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLETQRQHEFTDMAGTTAVPAGKTK"
     gene            44996..45223
                     /locus_tag="ACHJ69_000863"
     CDS             44996..45223
                     /locus_tag="ACHJ69_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO5013912.1"
                     /translation="MDDSDCAQAVMERATERALCDRLTRKRRVAETPGRRVCADCGGD
                     IPAARLVAVPDAIRCVNCQNIMEAGHVGQHR"
     gene            45204..45515
                     /locus_tag="ACHJ69_000864"
     CDS             45204..45515
                     /locus_tag="ACHJ69_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFO5013913.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VERVESRISGLPTQQEVNRLNIEITTLRGELKATNATLKSVSHQNELLLEQAVRKKHN
                     E"
     gene            45508..45798
                     /locus_tag="ACHJ69_000865"
     CDS             45508..45798
                     /locus_tag="ACHJ69_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFO5013914.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRTLVNGYFIAELTGRGQDVAEGRATVPGVKKPRARG"
     gene            45801..46382
                     /locus_tag="ACHJ69_000866"
     CDS             45801..46382
                     /locus_tag="ACHJ69_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HFO5013915.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQIEFRIRQMLLGKA"
     gene            46393..46641
                     /locus_tag="ACHJ69_000867"
     CDS             46393..46641
                     /locus_tag="ACHJ69_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013916.1"
                     /translation="MVTDNRSLMSSAHQKAFSLATQLPPETIKACRELTELSIAGEIS
                     LEQLHSLLAGAVTDELSGVDVMTEELEFRIKSILTGKA"
     gene            46641..48305
                     /locus_tag="ACHJ69_000868"
     CDS             46641..48305
                     /locus_tag="ACHJ69_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5013917.1"
                     /translation="MNKAKEKSPSLIQMTPPRKIDLSGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFADESQVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAVALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTDQEWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMIRDIPILTFEAPDDFISRAAWLRESEVLTWCEEH
                     LKPLTEKLNPRSRFSFGEDFARTGDLSCFVLLEITELLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAANARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            48305..49894
                     /locus_tag="ACHJ69_000869"
     CDS             48305..49894
                     /locus_tag="ACHJ69_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HFO5013918.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSTALDIPQIASRYVEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTAHAIDIRRLPRICFQTKEPGDITKIASAVMQLSAGMDIP
                     DPWVRDQTGIPQPTPGEAIFRVRQSSNEPAMPDRETLPEKQEKTEQTALSARLPEAKN
                     TPRDELDDMGDAVPARRLQEAIDPVLEPVIDVIRTRGLAEALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDGSGD"
     gene            49878..51209
                     /locus_tag="ACHJ69_000870"
     CDS             49878..51209
                     /locus_tag="ACHJ69_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFO5013919.1"
                     /translation="MAQGIDLGYAATLPSKETVAYFRAKGAHISWNWFETDADVHARS
                     FTAAKTARLDVVTTLQAEVQRAIDEGISQKEFIRTLTPRLQKLGWWGKQIVVDSDGNA
                     EEVQQGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPSHSAL
                     NGLVFRYDDPFWKTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGEDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGARTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMQTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSVKHQNDGIALTPEDFGRLPAMLARPKAVLWDKRHNNLMYIVENQDG
                     SVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGQID"
     gene            complement(51211..51290)
                     /locus_tag="ACHJ69_000871"
     tRNA            complement(51211..51290)
                     /locus_tag="ACHJ69_000871"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            51331..51804
                     /locus_tag="ACHJ69_000872"
     CDS             51331..51804
                     /locus_tag="ACHJ69_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO5013920.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVAASLLSSSE
                     MAFEQEKEPDGERWHDWSDPYREWRTRKGYTPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            51982..53115
                     /locus_tag="ACHJ69_000873"
     CDS             51982..53115
                     /locus_tag="ACHJ69_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFO5013921.1"
                     /translation="MKTENHPKDANRPSGLAVLSASLTASGDGWCQLLPAGRVKARDG
                     RPEKPTEGWLINESACNRMKAGLSALHQPLLIDYDHHSVNAQKNGFKAIAAGWVKPEN
                     IEWREGQGIFIKPEWTPQAQKHIDDREYAYLSAYMHYFADTGEPYLLRMASLTNDPGI
                     TGMNPVAALSADDLYVVSPSQEQSPMNEQLRQLLTALGLTVADGDEFTPELGTAALSA
                     LTGIKTRADAHDTLKTQVASLSAELETAKGTPTGGTIDLTKYVPVETYNALRTEYAAL
                     SAQHGSTTLEQLLDKAESEGRIFKSERGYMEGLGQQIGVAALSAQLDARQPVAALTTL
                     QTDTVTVPDKKTATAVLSAEDIAAARLLGKTEAEFLKMKEEMQ"
     gene            53115..54062
                     /locus_tag="ACHJ69_000874"
     CDS             53115..54062
                     /locus_tag="ACHJ69_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO5013922.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYQKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAISRDDFEDDNLGIYSP
                     LFQEMGRAAAAQPDELVFAALRDGLNAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMRDFKADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNATVSNEMKGKLELVVADYL"
     gene            54106..54465
                     /locus_tag="ACHJ69_000875"
     CDS             54106..54465
                     /locus_tag="ACHJ69_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFO5013923.1"
                     /translation="MNEHHTVAATDDSGLQVSGDSPVTVLAEVRCSRTHFRRAGFLFM
                     RGRQQVDVTPEQLARLEAEPCLTVRILQTPGDDAGAVAGVVHAETGADLAEAETVQDA
                     PRKKTGNNARQAKKARA"
     gene            54462..54881
                     /locus_tag="ACHJ69_000876"
     CDS             54462..54881
                     /locus_tag="ACHJ69_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFO5013924.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSSRYTLPLAVVPAVLVQHCCTIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTDGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            54878..55441
                     /locus_tag="ACHJ69_000877"
     CDS             54878..55441
                     /locus_tag="ACHJ69_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HFO5013925.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREVSRPGLYQIVARLITVLDGFRAE
                     KTSPLYFEKAVNGYTETQAGSGAVMYALYFSCEEMIDPLTDISSLDDFLRHYETFTEP
                     EGTPEFKAHIRLPGATAAGENAGLSEE"
     gene            55445..55723
                     /locus_tag="ACHJ69_000878"
     CDS             55445..55723
                     /locus_tag="ACHJ69_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFO5013926.1"
                     /translation="MTTIFIKPAPGCLIRDPDTMTPLSQDGEEKPFTPFWCRRLNDGD
                     VIVVDKTAEPAPAATTANTGAAAKPAGAVAEKTAAVAEKTAASDKGTS"
     gene            55723..57225
                     /locus_tag="ACHJ69_000879"
     CDS             55723..57225
                     /locus_tag="ACHJ69_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO5013927.1"
                     /translation="MINFDYIGDNNRIPLVQIEINNSMAVTGTPPQRQAVLLFGQAAM
                     KDTTIQGRGQLDVPVRITRASQARELWGRGSMIALMVEEFIAINPDTELYAIAQGAGT
                     GQSRACVMNIIGTALADGVLSVYIGGRRYLLPVSKGKKGKELVEELAKVINADTDAPF
                     TAIAAEVSGDNAEGLKGSMGVNARFIGECSAHDLRVNYYDGETTPAGLTLELVAPTQK
                     ATNPDITRSVSGMGNRQYNYIVMPYKDAANLKILADELLKRWGPVKMSDGMVWMAHTG
                     TFGTATSFGESRNDFLFTCTSIPKAPEPDYVWAASICAVCAPSLSTDPARPLQTLALP
                     ARMAPEAPDRLTREERNSLLYTGMSTVTVAAADVVQIERQVTMYRQNAYGEIDPSYLD
                     VETIYTLSYLRYSLRTFITQRFPSHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWV
                     DLGLVENLDTFKKNLLVERNTKDRNRLDVMCTPDLVNQFRFMAAQIRFIL"
     gene            57234..57599
                     /locus_tag="ACHJ69_000880"
     CDS             57234..57599
                     /locus_tag="ACHJ69_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO5013928.1"
                     /translation="MSGKQYQGTATIRVNGQEYDTLEGATFSPSGFEREVVKGAKVYG
                     YREKPREATLDCKFPAGGEGSPAADEINTWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGDISAKFASATSTRVQ"
     gene            57614..58090
                     /locus_tag="ACHJ69_000881"
     CDS             57614..58090
                     /locus_tag="ACHJ69_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO5013929.1"
                     /translation="MSTRKKKTAVSDEAVMEAIRDALEDGDPRTAGLAEQLANGYVDL
                     LDGLPFGESREYRVTFRELTAKDSIDAESEAERLMETRNGPVLVASPALRGIALLRRQ
                     IAAVGQIEGPLSPRQIGQLSERDLSRLMAAVSLLDSAMAGKLVAERGRPDAVPGSD"
     gene            58218..60275
                     /locus_tag="ACHJ69_000882"
     CDS             58218..60275
                     /locus_tag="ACHJ69_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO5013930.1"
                     /translation="MAKNLKASLIVDLLGNISAKSRQWSQELGAFSRSGRTGLGGLGN
                     AARRAGQETELVGSRMQRTLAGVRGSIRTVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNKAFIRPAAEMENYILRLNAINHGDTAKTEAVKAWAVQNAKDTTWGLAGVMQ
                     EYASSRGFGMSDREARRFITMLQDQGGYHGWSLSDAQGASLQLKQMFARQSIQAADAN
                     ILTGYGINVYQLLADKLGVNQKIIREKGEKGKLGPDSIRLLFQVMAEQAKGAQKNAMN
                     SWTGMTSMMGDVWDGFAREVMAKGPFDSLKKSLKGFLDYADAAQKSGLQDKLATQTAS
                     ALNQGFEYARDAATGFYRVIQKVRETLQALRDAGYGDALDRIGQGAQTAAKYLMYMYL
                     ASRALKVLRFAGTGALRLGATPLRYGMAMTSVLTSPFRKPQTTVPGTQPGRAGRFLNF
                     LTGVNPAAVQPVLVTNWPAGGLASGGGDVVVSGDGKTVRGRKKRGPGRGRGVTTVVTA
                     GEQLAESAGKQGFFGRMMSRAGGLLTAAGNRMGLGRFAGLFRGAGRLGGGALWAGAMA
                     APVLLDSSASAADKAGAVGSLAGSIAGGALGAAAGPVGVAIGSTVGSYLGDYLGGWLT
                     QAWQKLRGGSDENGGQATAKTAARVELVAPEGWRARSIDVDDTAQHGLDVNVWNGGNY
                     GLY"
     gene            60262..61620
                     /locus_tag="ACHJ69_000883"
     CDS             60262..61620
                     /locus_tag="ACHJ69_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HFO5013931.1"
                     /translation="MASTDTPGRGSFRGVPFLVYQEQRERGGRNIVRREYPLRESGGA
                     DDLGPKLPEFTFTVLVTGDDLQTQRSRLRDALRAPGAGELMHPDYGTLNVLINSFESR
                     YNASEQGTVEFTINVIPASDDTAPTVAEDTAAALEQKSGSAMNQLFNTLSDGWTVISD
                     GLHDVQAMTDTISDKIDALENAVSGMGIVQDISAFTASFTALKGNAAALINAPRRMAE
                     SLAGMFAVLTGLPGDPSLSLTGKAGTPSGSLTTNRDSMAEQAMPQLYRTLSSLRYTLS
                     EQDDPQRLIGLTPAAQKNIRLLRSVMQSATLVSQAQTLGKLLDQVIRQNTTHVQDTDR
                     SGGLAWLESSADVQRINRDLSDAMEQQVLDLSAQGYTGTALALRDAGLALTEDLTTRS
                     VHLPGAFQVMVRTTEPALVTLYRATGNSSRWQYFVRRNNIPDPVFVPGGRSVEVISEQ
                     QD"
     gene            61604..62728
                     /locus_tag="ACHJ69_000884"
     CDS             61604..62728
                     /locus_tag="ACHJ69_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFO5013932.1"
                     /translation="MSSRIELYIGGSIFSGWLTVSVRRSLEHLAGSFELGLMLPGERI
                     PSALRTGQSLTLRINGQTVISGWLDQVSQRISATRHQISISGRDKTGDLVDCAAIHPG
                     SQWRNRTLAQIAADLCAPFGIAVRWQVNDDTAARPFSSFTLENSETVADALTRAARHR
                     GVLVTSNADGDLVFTQAGSQQTDRLVLGDNLLDADYNTDWRGRYSEYRVRGHGRGGGK
                     RGDSESAARLAAPVGVISDEQIGRYRSKIILADQQTDTTGARQRALREMRRAIARSER
                     FSATVRGWFRDDGRLWDVNLLTGVSALRFGIEQTELLVCQVEFLLDEHNGEVTRLVLA
                     PRDGFIVPAEPDSKGRGGSGDDVDAFIRQQMKKQGISFDE"
     gene            62718..63332
                     /locus_tag="ACHJ69_000885"
     CDS             62718..63332
                     /locus_tag="ACHJ69_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO5013933.1"
                     /translation="MMNDEVFSRLIAPVTRGIRLLFGRGVLTGTHDELKMQNVQLTGM
                     DGETFDDVERPQQYGQISVPLPGAETFFACLGGQRDQTVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKDGRIIVTCKMLEIYADEGMRVDTPEATFTGNVTVDKNLHVKGNF
                     VLDGTGKSDGLFTMSDAVIAGITYSGHVHQDNGKGSKTGAPENG"
     gene            63325..63762
                     /locus_tag="ACHJ69_000886"
     CDS             63325..63762
                     /locus_tag="ACHJ69_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFO5013934.1"
                     /translation="MADIAIVWDQGCGSLQLNGADLLTDDSLLTAVLISLFTDRRALA
                     SDEIPDGTRDRRGWWGDSFRPRPIGSRLWLLRREKTLASVISRARAYADEALGWLSQD
                     GVASSVVCHAERVGHARLALSVRITLPDGSVRPMIFYADLKGQ"
     gene            63762..64844
                     /locus_tag="ACHJ69_000887"
     CDS             63762..64844
                     /locus_tag="ACHJ69_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO5013935.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYAQAG
                     LSAQEHEHLAWIARQIIPTEADEAELLKHCAFWGVMRKPASRGDGPVQLMLTTDAGIK
                     EGVLLQRSDGVVYRITTSLTGKAGTMNVSVEAESAGRAGNAPAGTKLTFITPQAGINQ
                     TATVTGTGITGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCRP
                     EWPQAGSVGVTFVQDNNPDIFPGDGDVQRVADYIRSHDDPATGQPVGQPLGPTVTVFK
                     LTNKPVPFSIRIIPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKNLED
                     FEVRSPLTSVQAGDSELLTVGEITWL"
     gene            64835..65395
                     /locus_tag="ACHJ69_000888"
     CDS             64835..65395
                     /locus_tag="ACHJ69_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFO5013936.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLASLCLGLSHSTARV
                     SWTGKQLLAERFPDTSRLLLEDWERYLGLPECDMAGATLTERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYQIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEETI"
     gene            65395..66276
                     /locus_tag="ACHJ69_000889"
     CDS             65395..66276
                     /locus_tag="ACHJ69_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5013937.1"
                     /translation="MFHVDNNSGVANMPALAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGTLATKNSLGPADVNALAKDQNLADLENKGTARNNLDVYSKSEGDNRYLRR
                     EQNGADIPDKGAFIDNVGLRETVNRAANALPSDGTAVAANRLANAHTINGVPFDGTQD
                     ITISSGTVTAIRLGSVTAHMPGTWESWDLNLGGGNVLTGIKVQDVGKNTADNVGGVYY
                     RPLQYLLNGAWVTAASI"
     gene            complement(66308..66829)
                     /locus_tag="ACHJ69_000890"
     CDS             complement(66308..66829)
                     /locus_tag="ACHJ69_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO5013938.1"
                     /translation="MELKNITRYYPENMPYGNNVQYFQSEDGKDFYESLPLFTKKYKL
                     CITPDSGVICSISQDASALYPAGFSVVEVDELPEGTDISGNWKFDNGIISRIPVNYAR
                     KLEAMRQSYLNQAYEKINDWRTELQLGTISDEDRAALTQWMAYISQVKKMELPAIKTE
                     AEFNAIKWPEQPQ"
BASE COUNT        16353 a        16309 c        18410 g        15758 t
ORIGIN      
        1 ggcgacgaag ccaccgtgaa caataacggc agcaccaccg ttgacggcaa ggattccacc
       61 ggcaccgaga tcgcaggtaa taacgccacc gttactcagg aaggtgaact cacggttagc
      121 agcggcggtc gcggtatcga catcacaggc aacaatgcca aggtcgacac taaaggcaaa
      181 atgaccatca caggtactga ctcggttggt gtgtccatca atggcgacag tgcgacgttg
      241 accaatacgg gcgatattga cgtcagcaat tcggctaccg gattcagcct cgtaaccaat
      301 gaggggatta tctcgctggc tggcagcatg aaagtgggcg atttttcaac gggcatggcg
      361 cttagcggcg acaacaacag cgtgacgctg gcggcgaaag atatcaatgt caccgggcag
      421 aaagcgacag gcgttaacat ctcaggcgag agtaataccg ttgatatcac cggtaatatt
      481 ctggtggata aagaccaaac cgcagataac gcggtagttt atttctacga cccatccgtt
      541 ggtgtgaata tcagcggcaa cagtaacacg gtatcactgg acggtaagct aaccgttatt
      601 gcagatagtg agttaacctc acgcaaatac atggagtttg atggtagcca ggagaatatt
      661 tctgggctta ccgtgtctgg cgatggtaac accgttaatt tgaatggcgg cattcagttt
      721 gtgggcgaga aaaacgcgct ggcagacggt tcaactattg ccgataagcg cagctacttt
      781 ggtaagacac ctcttgttag tgtcgatgga caatccaaag tttatctgaa cggtgattct
      841 actatcagcg gctctttgcc gttaggctat gccaacattc ttcagctaag caataaggcg
      901 gcgcttgaaa ttggtagtga cgctacgttc agtatgcagg acattagtgt ttacgagcac
      961 tatttcacgc aaactccgca aattatcaag gttgatacgg gttcacaggt cgtcaacaat
     1021 ggtgatgtgg atatctggaa tatcagtttt gcaggcattt ggggcgaaaa ctctaccggt
     1081 attaacaacg gaaatattac gctttctcaa tatgactatt cttctccgga gacatcgttc
     1141 tcagaacctg accacatggc ctttttaagc tccagtggcg gtagtgctgt gaacaacgga
     1201 acgatcactg caaaagtgat ggaacagcac agtgttctca atctgggctc tgcagccggt
     1261 gttgcagacc cgagggtatt caataatagc gtgtcgagta tgatgggaat ggaggcctac
     1321 ggtaaaggca ctgttcttaa cagcgaaagc ggcgttattg acatgtacgg gcgcggcaac
     1381 attggtatgt tggcagttga tgactctgca gcggataacg ccgggaaaat cacgctggat
     1441 acgctttggg tcgatcaaaa cgacaccacg acgctgcgta ctgacctccc ttccagcacg
     1501 gctatagatt acggtgttgg tatggccacc ggaacgaata gtggtggtgg cgcaagaagc
     1561 aacggtgttg cgactaacca acaaggcggt gtaatcactg tttataatgc cggtgctgct
     1621 atggctgctt acggtgccag caacatggtt attaaccagg gcattatcaa tctggagaaa
     1681 aacggaaatt acgatggcgg cttgggcgcg aatatgctcg tggggatggc ggtctataac
     1741 agaggcaccg ctatcaacga taaaacaggc gtgatcaata ttaacgtcga tacggggcag
     1801 gcgttttata acgacggtac agggacaatt ctgaactatg gtgagataaa ccttctcggc
     1861 tcgccaatgg acagtgcaga ctctcacatg ggggctattc cggagaatct ggatctgctg
     1921 acagcactga caggcagcgg cgaaacggat atgcgaactg cttcaagtgg cgggtttgtg
     1981 acgaccaaag cgctggcaaa ctacggcaat gaaacgctga atagcaatgt ggctgccaaa
     2041 gcgtggcttt ataaccagga taaagccaac ctgacaatta acggagagct atccatcggg
     2101 caagggctgg aaaacagcgg cttactcgat tctgacacca ttagcgcagc cgcgaatgtc
     2161 tataaccgag catcgggtag cattatcact gatcagttat cgctaacggg tagtaactcg
     2221 tttttcaatg agggcaactt cagcggttcg gttgctggct caagctataa gcaaaatgtg
     2281 gttaacacgg gaacgatggc tgtaatggcc gacggtaagt cgcttatcag tggcagcttc
     2341 ctgctttata acgaagcagg cgcaacactc agcaacagca gcagcgccgt ttcaggtgga
     2401 gaaaatgcca ttgttaacgt aacccgcacc ggcgattcac tcgcgcaagt taaccgtggc
     2461 accattactg cggtcaacgg ctacagtgca attaaaacgg caagtacggg ttctaacagc
     2521 aacgggaaat ggatctggaa taccgacacg ggggtaatta gcggcgtgaa tccaaatgca
     2581 ccgttaattg atcttggtcg cggctataac tttgccaacg ccggcactat taatgtgcag
     2641 ggtgatggtg cagtcgctat cagcggggga accaccagct atacggttca actggtgaac
     2701 agtgggacca tcaatgtcgg tacggcgcag ggtaaagcgg acggcactaa cggtactggg
     2761 ttaattggta tcaaggggaa tggcagtgat acgacaatta ataacgcaca aagcggtgta
     2821 attaacgttt atgcagataa ttcctgggcc ttcggtggaa agaccaaagc gatcattaac
     2881 aatggtgaaa ttaacctgct gtgtgatacg ggatgtgata tctatgcacc gggtaccacc
     2941 ggtacgctga acgatcataa cagtactact gatattatcg ttcctgcagc aacgagcaca
     3001 cctacgcagg gttcagtccc cacagtacct gcggactcca gtgcacagca aaaactgact
     3061 aactacacta ttggtaccaa tagcgatggc acctctggta tgttgaaagc caataacctg
     3121 gtcatcagcg acaacgtcaa ggtaaatacc gggtacagtg caggtactgc cgatacgact
     3181 gttgtgatta atgatgtttt caaaggtgaa aatatttccg gggctgagaa tatttcttct
     3241 tccacggtaa tgtggaatgc tcagggcagc actgatgcca gcggcaacgt agacgtcacg
     3301 atgaccaaaa acgcctatac ggatgttgtg acggattctt cagttaataa tgtcgctcag
     3361 gtactggatt cgggttacac caataacgac ctgtacacta gtctgaacgt aggaacgact
     3421 gctgaactga acagtgcgct gaaacaaatt agcggcagcc aggcaaccac ggtattcaat
     3481 gaagcacgag tgttaagtaa ccgcttcagc atgctgtccg atgcagcacc ggaagttgca
     3541 aacggtctgg ccttcaacgt ggtagcgaaa ggcgacccgc gtgctgaact gggaaataat
     3601 acacagtacg acatgatggc gctgcgtaaa tccatgacac tgactgaata tcagaatctg
     3661 agtctggaat acggtattgc tcgtctggag ggtaatggtt cagataccgt aggcgataat
     3721 ggtgtgactg gcggttacag ccagttcttc ggtctgaaac accagatggc gtttgacaac
     3781 ggtatgagct ggaataacgc cctgcgttat gatgtgcata atctcgatag cagtcgctcg
     3841 atagcttacg gcgacgtcaa caaaaccgct gatgctaatg tgaaacagca gtatctggaa
     3901 ttccgtagtg aaggggcaaa aacctttgaa ctgcgtgaag gtctgaatgt gacgccttat
     3961 gcgggtgtga aactgcgtca tacgctggaa aacggttatc aggagcgtaa tgcgggcgac
     4021 tttaacctga gcatgaacag cggcagcgaa acggcggtgg acagcatcgt tgggctgaaa
     4081 ctggactatg cagggaaaga aggctggagt gcgaacgcca cgctggaagg tgggccgaat
     4141 ctgagctatg tgaaaagtca acgtactgcg agtatttcgg gcgcgggaag ccagcgtttc
     4201 aacattgacg atggtcagag tggcggtggt ttcaatagtc tggcgacaat gggtgtgaaa
     4261 tacagcagcc aggaaagtgc attgcagtta gatgccttcc actggaaaga agacggcatc
     4321 agtgataaag gtgtgatgct aaacttcaag aaaacgttct aatttttagc atgtaataca
     4381 taaaccgcaa cgctgataca ggttgcggtt tttttattgc cggatgcggt gcttgacgtg
     4441 ttttgttttg gtgtctttca ggatattaga gctactcatc cagtaatctt gtttacgcct
     4501 tcgcattatt tatctctatt catttctata ctgatttttc ttaatccgtt ttattacagg
     4561 acagggtgcg atgagcagca acacatttac tctcggtaca aaatccgtta accgtcttgg
     4621 ttatggcgcg atgcaactgg caggtcctgg ggtttttggc cccccaagag atcgccacgt
     4681 cgctataacc gtgctgcgcg aggcgctggc attgggcgtc aatcatattg ataccagcga
     4741 cttttatggt ccgcacgtca ccaatcagat tatccgcgaa gcgctttatc cttactctga
     4801 cgacctgaca attgtcacta aaattggtgc gcggcgtgga gaggacgctt cctggttgcc
     4861 cgcattttct ccggcagagc tgcaaaaagc ggtgcacaat aatctacgta atctcgggct
     4921 ggacgtactg gatgtggtta acctgcgcgt tatgatgggg gatggtcatg gcccagcgga
     4981 aggatcgatt gaagccagcc tgaccgtgct ggcagagatg caacaacaag gcctggtaaa
     5041 acatattggt ctgagcaacg ttacaccgac gcaggttgca gaggcgcgta agattaccga
     5101 aatcgtctgt gtgcagaacg aatacaacat tgctcaccgt gctgatgacg caatgattga
     5161 tgctttggct cgtgacggca ttgcctacgt gccgttcttt ccgctcgggg gctttacgcc
     5221 gctgcaatcc tccacgcttt ccgatgttgc tgcgagcctg ggtgcaacac caatgcaggt
     5281 ggcgttggcg tggctgttac agcgttcacc gaatattttg ctgattcctg gaacatcttc
     5341 tgttgcgcat ttacgggaga atatggctgc tgaaaaaatt gcatctttct gagaaagtgt
     5401 tgtctacgtt ggatggtatt tcgcgagaat aacgaatata caaaagggaa agatgcattt
     5461 cccttttgtt tcttttttaa tggcatggag tgcatatgtt taaggccagg atttgcggct
     5521 ggataatcag ggaaaaggcg atggattcat tgattcgtac agtgatagat ggtatctcac
     5581 tgatatccag acggtaatca aacagatgtg tagggcattc atccaccaca tcttgatata
     5641 ttccgaattt acgttgatgc tgcgcgttgt gatatctccg ccgtcggaat tgatgcacca
     5701 tagagcaaac tgaatatcac ggaatgataa atgacgctct accagataaa accgctcttt
     5761 cagtcgctgt taaggccgac gatgttttgg ctttataagc accatgttac agcgaatcac
     5821 atcactcttg ctgcactggc gctttctctt cttaccggat tgctgttggt gttggtggca
     5881 caacccatcc tctttttgct attgcccatc gtgcttttta tccgtatgac gctcaatgcg
     5941 ctggatggca tgttggcgcg tgagtgcaat cagaaaacac gtctgggggc gattttgaat
     6001 gaaactggcg atgttatttc cgatattgtg ctctatttac cgtttttatt tttaccggaa
     6061 agtaacgcat cactcgtaat actcatgtta ttttgcacca tattgactga gttttgcggt
     6121 ttactcgcgc agacgattaa cggtattcgc agttatgccg gtccatttgg caaaagcgat
     6181 cgcgcgttaa tatttggtct gtgggggcta gccgttgcca tttatccaca gtggatgcaa
     6241 tggaataatc ttttatggag cattgcttca attctgcttc tctggactgc gattaatcgt
     6301 tgtcggagtg tgctttttat gagtgcagaa agataatgct ggaaaaatct ctggcaacac
     6361 ttttcgcgtt gttaatttta gcaacgctga taaaccgttt tctggtatgg cggttaccgg
     6421 agagaaaagg gggtgaggtt acattacgta ttcgtacctg gtggggcatc gtcatttgtt
     6481 tttcaatggt gatttcaggt ccacgctgga tgacgttgac gttttttgcg ctgataagtt
     6541 ttctggcatt gaaagaatat tgtacgctta tatctgtaca ttttccgcgt tggttatatt
     6601 ggggtattcc tcttaactat ttgcttatcg gttttaattg ctttgagctg tttctgttat
     6661 ttataccttt ggctgggttt ctgatattag ccacctggcg agttttagtg ggtgacccct
     6721 ccggttttct gcataccgtg agcgccattt tttgtggctg ggtaatgacc gttttcacct
     6781 tgagtcatgc cgcctggtta ttaatgttgc caaccataaa tatccagggc ggggcgttac
     6841 tggtgttatt tcttttggcg ttaaccgagt caaacgatat tgcacagtat ttatggggaa
     6901 aatcctgcgg cagaagaaaa gtggtcccca aagtcagccc gggaaaaacg ctggaaggtc
     6961 tggtgggggg agtaatcacc accatgattg cgtcactgat tattggaccg ttactgacac
     7021 cgctaaatac attacaggta ttattagcgg gtttgttaat tggtattagt ggtttttgcg
     7081 gcgatgtagt gatgtcagcc accaaacgag atgttggtgt taaagatagc ggaaaactat
     7141 taccaggaca tggcggactc cttgacagga ttgactcatt aattttcacc gctccggtat
     7201 ttttttattt tattcgctac tgctgttact gaaggaaaat agagaaaatg gaaaattcac
     7261 gcatccctgg gggacatttt tttaccacca gtgataatac agcgttgttt tatcggcact
     7321 ggcccacttt acagccaggg gcgaaaaagg tcatcgtctt atttcatcgc ggtcatgaac
     7381 attctggtcg tctacaacat atcgttgatg aactggcgat gccagatact gctttttatg
     7441 catgggatgc ccgagggcat ggacaaactt cggggccgcg tggttatagt ccgtctcttg
     7501 cgcgttcagt gcaggatgtt gatgaatttg tccgttttgc tgccagcgac agccaggtcg
     7561 gactggaaga ggtggttgtg atcgcgcaaa gcgtcggcgc agtgatggtt gccacatggg
     7621 ttcatgatta tgcgcctgct attcgcggac tagtgctggc atctccggcc tttaaggtta
     7681 aattgtatgt gccgctggca cgtcctgggc tggcgttatg gcatcgtctg cgtggtctgt
     7741 tttttattaa ttcctatgtg aaagggcgtt atctgaccca cgatcggcaa cgggtggcga
     7801 gtttcaataa tgatccgctg atcacacggg cgattgccgt caatatcttg ctcgatctct
     7861 ataaaacgtc tgaacgtatt gttagcgatg cggcggcgat tacgctctcc acgcaacttc
     7921 tgatatcagg cgatgactat gtggtgcatc gccaaccgca gattgatttt tatcagcgtt
     7981 tacgtagtcc tctgaaagag ctgcatctgc tgccaggctt ttatcacgac acgttgggtg
     8041 aagagaacag ggcgcaggca tttgaaaaaa tgcaaagctt tatcagccgt ttatatgcta
     8101 acaagtcgca aaaatttgat tatcagcatg aagaccgcac aggaccatca gcggatcgat
     8161 ggcggctcct ttctggtgga cccgtgccgt tatcgccggt tgatttggcg tatcgcttta
     8221 tgcgaaaggc gatgaaactg ttcggggtgc actctgcggg cctgcatctc ggaatgagta
     8281 ccggctttga ttcaggcagt tcgctggatt atgtctatca aaatcaaccg caaggcagta
     8341 acgcattcgg gcgctttatc gacaaaatct acctgaacag tgttggctgg cgcggtattc
     8401 gccagcgcaa aacccattta caaatgctga ttaaacaagc cgttgcccat ctccacgcca
     8461 aaggtttagc cgtccgcgtg gttgacattg ccgcagggca tgggcgctat gtactggatg
     8521 cgctggcaaa cgagcccgcc ataagcgaaa ttttgttacg tgattacagt gaggtaaatg
     8581 ttgcacaagg gcaagagatg attgcccaac ggggaatgtc tgggcgggcg cgttttgaac
     8641 agggcgatgc gtttaatccg gcagaactca gcacgttaac tccgcggcct acgctggcga
     8701 ttgtctctgg cctgtatgag ctttttcccg aaaatgagca ggtaaaaaac tcactcgcag
     8761 gtcttgccaa tgccatcgat ccgggtggca ttctcatcta caccgggcag ccgtggcacc
     8821 cacaactgga gctgattgcc ggggtgttaa ccagtcataa agatggtaaa ccgtgggtaa
     8881 tgcgcgtgcg ttctcaaggg gagatggatt cgctcgtgca tgataccgga tttgataaat
     8941 gcacacaacg gattgatgag tggggcattt ttacggtttc gatggcggtg cgtcgtgata
     9001 actgaacgcc ggaacgtatt gctacaagga gctggctggt tattgttgct ggccccgttt
     9061 ttcttcttta catatggatt tcttaatcag ttcaccgcga ctcaggatct taacaaccat
     9121 gatatcccca gtcaggtatt cggttgggaa acggcgatcc cttttcttcc ctggactatt
     9181 gttccttact ggagtctgga tcttttatat ggattttcgc tgttcatttg tagctcgaca
     9241 ttcgaacagc gccgactggt tcaccggctt attctggcaa cggtaatggc ctgctgcggt
     9301 ttttttctct atccgctgaa gtttagtttt atccgtcctg aagtgagtgg ggtgacagga
     9361 tggctatttt cgcaacttga actgtttgat ctgccttata accagtctcc ttcgctgcat
     9421 attattctct gctggctact ttggcgtcac tttcgtcagc atctggctgt gaggtggcgt
     9481 aaagtctgcg gcggatggtt tttactcatc gccatttcga cgctgacgac ctggcagcat
     9541 cattttattg atgtcatcac ggggctggcg gtaggtatgt taattgactg gatagtgccc
     9601 gtcgaccgtc gttggaatta tcagaaacct gatcaacgtc gaatcaaaat agcactgcca
     9661 tatgtcgtag gcgcgtgctc gtgcattgtg ttgatggagc taatgataat gcttcagtta
     9721 tggtggtcag tctggttatg ttggccagta ttatcgctat tcatcattgg ccgtgggtac
     9781 ggtgggcttg gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt
     9841 tactggctga cattgccctg gcgtatcggg atgtggctgt ctatgcgttg gttttgtctt
     9901 cgcctggagc cggtgagcaa aattactgct ggtgtttatt taggggcgtt tccacgacat
     9961 attccggcac agaatgcggt tctggacgtc acctttgaat tccctcgcgg acgagcgaca
    10021 aaagatcgac tctatttttg tgtaccgatg ttggatctgg tggttccgga agagggggag
    10081 ctccgacagg ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc
    10141 cattgcgcgt tgggattatc gcgcagtgcg ctggtggtgg cggcatggct gttatgttac
    10201 ggacattgta aaaccgttga tgaagcgatt agttatattc gagccagacg ctcgcggatt
    10261 gtgcttaagg aagagcacaa agcgatgctg aaattatggg aaaacaggta agcggattga
    10321 gatgtggact gaatatctac agtccacatc aagatcgtgt ccggttattc agcaacaata
    10381 ctgtcgatgg ctgctttcgc gtcagactgc gctttcgctg ccatttccgg gccgtacgcg
    10441 atcccttcgg cgaagacaaa tttcacatcg gtaatgccga taaagcccag gaatgtggac
    10501 agatatggcg ttaccagatc cgttgggcca tctttatgga tcccaccgcg gctggtgata
    10561 acgatggctt ttttaccggt gaccaggcct tccggaccgt tctcggtata gcggaaagta
    10621 acgcctgcac gtgcaaccag gtcaaaatag tttttcaact gggtcgagat attgaagtta
    10681 tacatcggtg ccgcaataac gataacgtcg taggctttta gctcggcaat cagctcatcg
    10741 gaaagcgcca gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagtgca
    10801 ccaaccagtt cgccatccag gaccggaatc ggatttgcag ccaggtcacg cacggtgatt
    10861 tcatcagtgg agtgcttttc gcgccattgt tcaacaaaat aatcggacaa ctgattagac
    10921 tgagagtacc ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct
    10981 tatagatatt tgaatgggca atgccccgtt gcttgttgac actttattca caattctgcc
    11041 acagagatag cgcaataaat cgaagcctat gttcgaattt attgaacaac gcatagaaag
    11101 ccgcgatgtg gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc
    11161 cactaacgtt atgacagaac aacaaaaatt gacctttact gccttgcagc agcggctgga
    11221 ttcgatgatg ctgcgtgaca gactgcgttt ttctcgccgt ctgcacggcg tgaagaaggt
    11281 taaaaatcct gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccatgc
    11341 ggcagggaaa gtcctgctgc gtgaagcggc acgaccggag attacttacc cggacaattt
    11401 gccggttagt cagaaaaagc aggacattct cgaagcgatt cgtgaccacc aggtggtgat
    11461 cgtcgccggg gaaacgggtt ccggtaaaac gactcagcta ccgaaaatct gtatggagct
    11521 gggtcgcggg attaaagggc tgatcggcca tacccagccg cgtcggctgg cggcgcgaac
    11581 ggtggcgaac cgtattgcgg aagagctaaa aacggagccg ggcggttgca tcggttacaa
    11641 agtgcgtttc agcgatcacg tcagtgataa cacgatggtc aagctgatga ccgacggtat
    11701 cctgctggca gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga
    11761 cgaagcgcac gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct
    11821 gccgcggcgt cctgacctaa aaattattat cacttccgcg actatcgacc cggaacgctt
    11881 ttcgcgccac tttaataatg cgccgattat cgaagtctcc ggtcggacct atccggtgga
    11941 agtgcgctat cgcccgattg ttgaagaagc cgatgatacc gagcgcgatc agttgcaggc
    12001 gatttttgac gccgtagatg aactgagcca ggaaagccct ggcgacattc tgatctttat
    12061 gagcggcgag cgagaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca
    12121 taccgaaatc ttgccgcttt atgcacggct ttccaacagc gaacagaacc gcgtgttcca
    12181 gtcgcacagc gggcgacgca ttgtactggc gaccaacgtc gcggaaacct cgctgaccgt
    12241 accgggtatt aaatacgtta tcgacccagg tacggcgcgt atcagccgct acagctatcg
    12301 caccaaagtg cagcgtttgc cgattgagcc aatttcgcag gcttcagcta atcagcgtaa
    12361 aggccgctgc ggccgtgtgt cagaagggat ctgtattcgt ctctattccg aagacgattt
    12421 cctctcgcgc ccggagttta ccgacccaga aattctgcgt actaacctgg cctcggttat
    12481 tttgcagatg accgcactgg gcttgggcga tatcgccgca ttcccgtttg tcgaagcacc
    12541 ggataaacgc aatatccagg atggcgtgcg tctgctcgaa gagctgggcg cgatcaccac
    12601 tgatgaacag gccagcgcct ataaactgac gccgctcggt cgccagctct cgcaactgcc
    12661 agtcgatcct cgtctggcgc gaatggtgct ggaagcgcaa aaacatggct gcgtgcgtga
    12721 ggcgatgatt atcacgtccg cgctctctat tcaggacccg cgtgagcggc caatggacaa
    12781 gcagcaggca tcggacgaaa aacatcgtcg cttccacgac aaagagtctg acttcctcgc
    12841 gtttgtgaat ctgtggaatt atcttggcga gcagcaaaag gcgctctctt caaacgcctt
    12901 ccgtcgcctg tgtcgtaccg attatctcaa ctatctgcgc gtgcgcgaat ggcaggatat
    12961 ctacacccag ttgcgccagg tggtgaaaga acttggcatt ccggttaaca gcgaaccggc
    13021 ggattatcgc gaaattcaca ttgcgttgct gaccggtttg ctttcgcata tcggcatgaa
    13081 agatgccgat aaacaagaat ataccggcgc acgtaacgcg cgtttctcca tcttccccgg
    13141 ttctggttta ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag
    13201 ccgcctgtgg gggcgcattg cggcacgcat cgatccagaa tgggtggaac ctgtcgctca
    13261 gcatttaatc aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat
    13321 ggcaacggaa aaagtcaccg tttacgggtt gccgattgtt gctgcgcgta aggtcaacta
    13381 cagccagatc gatccggcgt tatgtcgtga actctttatt cgccacgcgc tggtggaagg
    13441 tgactggcag acgcgtcacg cattcttccg tgaaaacctg aaactacggg ccgaagtgga
    13501 agaactggaa cacaaaactc gtcgccgcga tattctggtg gatgacgaaa cgctgtttga
    13561 gttctacgac cagcgcatca gccacgatgt gatttccgca cgtcacttcg acagctggtg
    13621 gaaaaaagtc agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa
    13681 agaaggggcg gaaaaaatca gcaaactgga ttacccgaac ttctggcatc agggcaatct
    13741 caaactgcgt ttgagctatc agtttgagcc cggcgcggat gctgacggtg tgaccgtgca
    13801 tatcccgctg ccgttactta accaggttga ggaaagtggg tttgagtggc agatccccgg
    13861 tctgcgtcgt gagctgatca tcgccctgat taaatcgctg ccgaagccgg tacgccgtaa
    13921 ttttgtgccc gcgccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggaatt
    13981 gccgttgctc gacagccttg aacgagagtt acggcggatg actggcgtca ccgttgaccg
    14041 cgaagactgg cactgggatc aggtgcccga tcacctgaaa atcactttcc gcgtggtgga
    14101 tgacaaaaac aagaagctaa aagaagggcg ctcattacag gatctgaaag acgccctgaa
    14161 aggcaaagtg caggaaacgc tgtctgccgt ggcggatgac ggtatcgagc agagcggttt
    14221 acatatctgg agttttggtc aactgccgga aagctacgag cagaagcgcg gcaactacaa
    14281 agtgaaggcg tggccggcgc tggtggatga gcgtgacagc gtagcgatca aactgtttga
    14341 taacccgctg gaacagaagc aggcaatgtg gaacggtctt cgccgtttac tgctgctaaa
    14401 tattccatcg ccgatcaagt acttgcacga aaagctgcca aacaaagcca agctggggct
    14461 gtactttaat ccgtatggca aagtgctgga gctgatcgat gactgtatct cctgcggtgt
    14521 ggatcaactg atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct
    14581 gcatgaaaaa gtccgcgctg aactgaacga cacggtggtg gatattgcga agcaggtcga
    14641 acaaatcctc actgcggtat tcaatatcaa taaacgcctg aaaggtcgcg ttgatatgac
    14701 catggcgctg ggactgtcgg acatcaaagc gcagatgggc gggctggtgt atcgcggttt
    14761 tgtcactggc aacggtttta aacggttggg cgacacgtta cggtatttgc aggcgattga
    14821 aaaacgactg gaaaaactgg cgattgatcc acatcgcgac cgtgctcaga tgctgaaagt
    14881 cgaaaacgtc cagcaggcgt ggcagcaatg gatcaacaaa ctaccgcctg cacgtcgtga
    14941 ggatgaagac gtgaaagagg tccgctggat gatagaagag ctgcgcgtta gttacttcgc
    15001 tcaacaactt ggcacgcctt atccgatttc ggataagcgt attttgcagg cgatggagca
    15061 gattagcggt taacactgct atttgcctga taaatctaaa acccggtaag catttagcgc
    15121 cgggtttttt attaattcta aaaacgataa gggaaaaaat tcaggagttc agaaaaatac
    15181 aattctctgc tgcaagatga ataatgttta tctacagcat ttccttaaaa aatatgtcag
    15241 gcttgcggag tggctgttaa ggaaatacga cttcctcctt tcagagtgct ccgcttctca
    15301 ctattatctc acgcagtatt cttaagggaa cgataaggag gaaccatgaa tattaccccg
    15361 tttccgacac tttcgccggc aaccatagat gccataaatg ttatcggaca gtggctggtg
    15421 caggatgatt tctccggtga ggtaccgtat caggccgatt gcgtgatcct tgcaggcaat
    15481 gcggttatgc cgactatcga tgcggcatgt aagattgccc gcgatcagca aattccttta
    15541 ctgattagtg gtggtatcgg tcactcgaca acttttttgt atagcgccat cgcacagcat
    15601 ccgcactaca acactatccg caccactggc agagctgaag ctaccatcct ggcggatatc
    15661 gctcatcagt tctggcatat tccgcatgaa aaaatctgga ttgaagacca gtcaacaaac
    15721 tgcggtgaaa acgcacgctt tagcatcgcg ctattgaatc aggccgtaga acgagttcat
    15781 acggctatcg ttgttcagga ccccaccatg cagcggcgca cgatggcgac gttccgccgt
    15841 ataaccgggg ataatcccga tgcaccgcgc tggttaagtt atcccggatt cgttcctcag
    15901 ttaggaaata acgcagacag tgtaatcttt gttaatccgt tgcaaggatt atggccggtt
    15961 gagcgttatc tatcgctact cactggcgag ttgccgcgtt tacgcgatga tagcgatggc
    16021 tacggccccc gcgggcgaga ttttatcgtt cacgttgatt ttccggcaga agtcatccag
    16081 gcatggcaaa cgctgaaaca cgatgcggtg ctcatcgagg cgatggaaag tcgctcgtta
    16141 cgttaaaaat tgcccgtttg tgaaccactt gtttgcaaac gggcatgact tctgactttt
    16201 atttctgcct ttaattcctt ttacacttgt ttttatgaag cccttcacag aattgtcctt
    16261 tcacgatgcc gtctctctga taattgatgt taattgacaa taaatttacc aaaaacaaac
    16321 atgcaaatca caggagtcgc ccatgtcagt acccgttcaa catcctatgt atatcgatgg
    16381 acagtttgtt acctggcgtg gagacgcatg gattgatgtg gtaaaccctg ctacagaggc
    16441 tgtcatttcc cgcattcccg atggtcaggc cgaggatgcc cgtaaggcaa tcgatgcagc
    16501 agaacgtgca caaccagaat gggaagcgtt gcctgctatt gaacgcgcca gttggttgcg
    16561 caaaatctcc gccgggatcc gggaacgtgc cactgaaatc agtgcgctga ttgtcgaaga
    16621 agggggcaag atccagcagt tggctgaagt cgaagtggct tttaccgcgg actatatcga
    16681 ttatatggcg gagtgggcac ggcgttacga gggcgagatt attcaaagcg atcgtccagg
    16741 agaaaatatc cttctgttta aacgtgcgct tggcgtgact accggcattc tgccgtggaa
    16801 ctttccgttc ttcctcattg cccgcaaaat ggctcccgct cttttgaccg gtaataccat
    16861 cgtcattaaa cccagtgaat ttacgccaaa caatgcgatt gcattcgcca aaatcgttga
    16921 tgaaataggc cttccgcgcg gcgtgtttaa ccttgtactc gggcgtggtg aaaccgttgg
    16981 gcaagaactg gcgggtaacc caaaggtcgc aatggtcagt atgacaggca gcgtctctgc
    17041 aggtgaaaag attatggcga ctgcggcgaa aaacatcacc aaagtgtgcc tggaattggg
    17101 gggtaaagca ccggctatcg taatggacga tgccgatctt gaactggcag tcaaagccat
    17161 cgttgattca cgcgtcatta atagtgggca agtgtgtaac tgtgcagaac gtgtttatgt
    17221 acagaaaagc atttatgatc agttcgtcaa tcggctgggg gaagcgatgc aggcggttca
    17281 atttggtaac cccgctgaac gcaacgacat tgcgatggga ccgttgatta acgccgcggc
    17341 gctggaaagg gttgagcaaa aagtggcgcg cgcagtagaa gaaggggcga gagtggcgtt
    17401 gggtggcaaa gcggtagagg ggaaaggata ttattatccg ccgacattgc tgctggatgt
    17461 tcgccaggaa atgtcgatta tgcatgagga aacctttggc ccggttctgc cggtggtcgc
    17521 atttgacacg ctggaagagg ctatctcaat ggctaatgac agtgattacg gcctgacctc
    17581 atcaatctat acccaaaatc tgaacgtcgc gatgaaagcc attaaagggc tgaagtttgg
    17641 tgaaacttac atcaaccgtg aaaacttcga agctatgcaa ggcttccacg ctggatggcg
    17701 taaatccggt attggcggcg cagatggtaa acatggcttg catgaatatc tgcagaccca
    17761 ggtggtttat ttacagtcgt aatgagtgaa agaggcggag gttttttcct ccgcctctgc
    17821 gcgtcagagt ttagcgaatt tttcgagggt gcgaataagc tgcgtgacga agccatattc
    17881 gttatcgtac caggcgaccg ttttcaccag ttgtaaatcg cccacggcgg taatttccgt
    17941 ttgcgtggca tcaaacaccg aaccgaaatg gctgccaatg atatcggaag agactatttc
    18001 ttcatcagta taaccaaatg actcgttatt gtttgtcgcc tttttaagcg cgttgttcac
    18061 ctcttcggca gtcacttttt ttccgagaat cgacaccagt tcagtgaccg aacctgtttt
    18121 caccggcacg cgttgcgcat gacctttcag tttgccgctc aattccggga tcaccagacc
    18181 aatggctttt gccgcccccg tagtgtgggg aatgatattt tctgccgctg cgcgtgaagc
    18241 acgtaaatct ttaccacgtg ggccatccac cagtgactgg gtgccggtat aggcatgaat
    18301 ggttgtcatc gtgccgactt ctatcccgaa actgtcatgc aaggctttgg ccatcggcgc
    18361 aaggcagtta gtggtgcatg acgccacgga aacaatggtg tcgttgtcat ccagagtgtc
    18421 gtcattgacg ttataaacga tggttttcat ttcaccggca ggggcggaaa tcaacacctt
    18481 cttcgcgcca gcatcaagat gcgcctgcga tttctcggcg gaggtataaa agcctgtaca
    18541 ttcgacaatg atttctgcgc ctttcgcttt ccacggaata tttttagcct ctttttcggc
    18601 gtaaaccgcg atacttttcc catcaacgat aagtgaatct tccgtataat caatgctcca
    18661 ggggaacggt ccgtagtttg aatcatgttt cagcaggtag gcgagaattt ttggggaagt
    18721 gagatcatta atagcgacaa cgtctatgtt gcttttgact tcaagtaatc gacgcaacac
    18781 cagtcgaccg atacgaccaa aaccattaat accaacttta ctcatggtct tctcctgtca
    18841 ggaacgtttg gatgaaaatt gatcctttcc aggcttagac caggatggcg ggatgggcaa
    18901 tctacattct tgcagtgaaa cgtaacgtac tgaaaacggg tgaacaatat ttaatgaaat
    18961 tttaagaaaa gcccgttatg ttaacggcaa attatgttaa agcaggaaat gttatgggaa
    19021 ataaatactc aaggttacaa atcagcattc actggctggt ctttttgctg gttatcgtag
    19081 cgtattgcgc aatggagttt cgtggtttct tcccacgtag cgatcggccc ctcatcaaca
    19141 tgattcatgt ttcctgtggc atctcaattc tcgtactgat ggtggttcgt cttttgttaa
    19201 gactgaaata cccaaccccg ccgattacac ctaagccaaa accgatgatg acgggactgg
    19261 cgcatttggg ccatttggtg atttatctcc tgtttattgc gctgccagtg attggtttgg
    19321 tgatgatgta taaccggggc aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt
    19381 cagaggccaa tttcgagcgg gttgatagct taaagtcgtg gcatgaaacg ctggcgaatc
    19441 tgggatattt tgtcatcggc ttgcacgctg cggctgcact ggcacaccac tatttctgga
    19501 aggacaacac acttctacgc atgatgccac gtaaacgttc ctgaaggata tttaaagaaa
    19561 acgcctgtac taaaaccgac ccgtggtaca ggcgaagaat acgggcctac atcggaagcg
    19621 cctattatat ttattcgtat gacaaataaa accataatcc ttgtctatac gtccatctgg
    19681 cttatcttta atcaattcac ccgatctttg atctcatcaa cggtatcaaa ataaaccagc
    19741 gtataacgtt caaatcgaac ataagattcg ataaatcatg gaggttatat gaaaaaactg
    19801 gcacttatcc tggttatggg aacgcttgtt tccttttatg ccgatgccgg gcgcaaaccc
    19861 tgttctggtt cgaaaggggg gatctcacac tgtacggcag gcggcaaatt tgtctgtaat
    19921 gatggttcta ttagagcatc gaaaaaaaca tgcactaact gaagtgtaaa aggggtgcca
    19981 tgagaaaatg aatccgctag tcagatgcgc ggggaaaatc cccgcgcttg cccttacctg
    20041 gacgtgcagg ccatgagcgc agcaacttcc ttatcaccgt cccggaaccg cagttcgtag
    20101 agcgattgtc gggtcagtgt gaatgtcaga atcgtgttac tctcgcaagg ccgtggattt
    20161 gccgttgtcg ccagcgaagt aagccgcagc gctcaggcgg cgaaagagat tgaaggcttg
    20221 atcagtgaat cagtcaggtt aattgacctg ggatcggatg aggtggcaac ggcagggaaa
    20281 accatgtgca ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc
    20341 accgcctcgg atgaacaaag tcgaggcata acgcaggtta gccaggcgat ttctgaaatg
    20401 gataaggtga cgcaacagaa tgcttctctg gtagaagagg cctcaacggc ggcggtgtcc
    20461 cttgaagaac aggcggcacg actaactgag gcggtggacg tattccgtct gaacaaacat
    20521 tctgtgtcgg cagaaccacg aggagctggt gaaccagtta gttttgctac ggtgtgaaaa
    20581 tgttaaagga gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag
    20641 cgttaatcat caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa
    20701 tcggttcgcc tgcaccatgt cccggaacag gcagggctac cagcgtggca tgacgcaggt
    20761 cgtcttttac agcgccagaa gggacaaacc acacgtaatc gtattcaacc gtaagttgac
    20821 gagatagcga agcagacagc gtttcgatac aacccgaagg aattttacat ccctggctct
    20881 gcactaatgc atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc
    20941 attccagcac ccggcttagc gttacgttct cctgaagtag cgggtgattg gggcggacaa
    21001 ccagcttcag cgattcaaga aacagcagtt cataattaag cccgttcatc agttcaggat
    21061 ctgacatccg accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag
    21121 ggttactcat tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta
    21181 taaccgaagg taatatccct agtgccgcag taggtagcgc accaaccctg acgacatcat
    21241 tattaagacc ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc
    21301 tgactgcatg cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac
    21361 gctcaaacaa gcgagcgccc gtcagctgct ccagttcatt caatgtctta gagagcgcag
    21421 gttgactcaa attaagggtt tcagccgcgc gccccaaagt tccttgttgt gcgacagcta
    21481 cgaatgtatg aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa
    21541 gcgatgttaa aaacgaagcg gtgtcgctga caagtgaagt tgttttatta tgataacttg
    21601 attgcaaaat attattaaca attaaagcaa ctatgttaca gcaaaatgga taatattgat
    21661 gttttcgcgg cgagatcaca gtttgtaaat tcttccagcc ggagtgaatg cggttaccta
    21721 cactccagat tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga
    21781 gattcgggaa cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcagta
    21841 cggcacgctg ctggaactgg tagctgatgt aaatctggcg gtgctggaaa acaatcctca
    21901 agtgcacgaa aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg
    21961 ggcgattcgc gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat
    22021 ggggatgtac ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac
    22081 agcatttcgg cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacctc
    22141 gttactccgt cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct
    22201 gcgtcagcgc gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagca
    22261 gggcggtttt aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg
    22321 ctggcaccag tcagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg
    22381 gttgattgct gatgtggtct gttttcctgg atgccatatc aaccatctga cgccacgtac
    22441 gctggatatt gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaagttct
    22501 gatcgaaggg ccgccgcgcc gcgaggtgcc gattttacta cgccagacca gctttaaagc
    22561 actggaagag acggtgttgt ttgcggggca gaaacagggc acacataccg cgcgctttgg
    22621 tgaaattgag cagcgtggtg tggcattaac gccgaaaggg cgacaactgt atgatgatct
    22681 tctgcggaac gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac
    22741 cttccgcact tttcctgaca gtgagttttt aatgcgtcag caagggctgg gatggttccg
    22801 gtaccgtctg acgccttcgg gtgaggcgca tcgtcaggcg attcatcccg gagacgatcc
    22861 acagccctta atagaacgtg gttgggtagt ggcgcaaccc attacctatg aagatttctt
    22921 gcccgttagc gcggcgggga tcttccagtc aaatctgggt aatgaaacgc aggcatgcaa
    22981 tcacggtgat gccagtcgcg aagcatttga gcaggcgttg ggggggccgg ttttggatga
    23041 gttccagctt tatcaggaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat
    23101 tctgaccatc cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca
    23161 tcgacataaa aaaaatgcca atttatgcat attctctgat ttcaacaatt ggattattaa
    23221 taaatattgt ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaatgacca
    23281 gcgcgggacc attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt
    23341 attaaaggtt caatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt
    23401 tctcaggcgg cattcgcggc cgattctgat attgccgacg ggcaaaccca gcgttttgac
    23461 ttctccattc tacagtcaat ggcgcacgac ttagcgcaaa cagcatggcg tggtgcacca
    23521 cgtccgttac ctgacacttt ggcgacaatg acgccgcagg cttataacag tattcaatac
    23581 gacgccgaaa aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagtttttc
    23641 catatgggaa tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat
    23701 ctggcgcgtg aaattcactt tcgcccggag ttgttcaaat acaacgatgc gggtgtggat
    23761 accaaacaat tagaagggca aagcgatctc ggttttgccg gttttcgcgt gtttaaagcc
    23821 cccgaactgg cgcgccgtga tgtagtatca ttcctcggcg cgagttattt ccgcgccgtt
    23881 gatgacacat atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac
    23941 agcaaagaag agttccccga ctttaccgcc ttctggtttg atacggtaaa accaggggca
    24001 accaccttta ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc
    24061 actatccatt gtgagaaaaa tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc
    24121 aaagacatta aacagctggg cattgcgccg atgaccagta tgttcaactg cggtactaat
    24181 gaacgtcgga tgtgcgacac cattcatccg caaatccatg actctgatcg tctgtccatg
    24241 tggcggggca acggcgagtg gatttgccgt ccgctgaaca atccgcaaaa attgcagttc
    24301 aatgcttaca ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgatttc
    24361 tcccattatc aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggaaccg
    24421 cgtaacaagt ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg
    24481 ctggataaca ttgtctgctt ctggcagcca gaaaaagctg taaaagcggg tgatgagttt
    24541 gcattccagt atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc
    24601 gttatggcga cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac
    24661 tatcccgaaa aatgggcgcg acgttttgcc gtcgatttcg ttggtggtga tctgaaagct
    24721 gccgcgccaa aaggcattga gccggtgatt acgctttcca gtggggaagc gaagcaaatc
    24781 gaaattctct atattgaacc cattgatggt tatcgtattc agtttgactg gtatccgact
    24841 tcggactcca ctgatccggt cgatatgcgg atgtatctgc gttgtcaggg cgacgctatc
    24901 agtgaaacat ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgat
    24961 gaccgcgtga tgagttaatc gttttttctt cggcatcttc ttcgggaggt gccgtctggt
    25021 taaacacgat cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt
    25081 tcagatacac cttgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt
    25141 aatatcccgt ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa
    25201 acttgatcat gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt
    25261 ggtccgcatg aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca
    25321 ggtgagcgtc aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacag
    25381 ctgcatggat tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa
    25441 ttacatgctg ttgcggaaag tcatgtcaca cctctttatc agttaatctg taaaaataaa
    25501 acctggttac agcagtcgct aaactggccg cagtttgttc aaagtgaaga ggacacgcga
    25561 aaaacggtgc agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt
    25621 ttcaaagaag atgaacttat cggcgttatc tcttttaatc gtattgaacc actgaataaa
    25681 accgctgaaa tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag
    25741 gcgctgcagg cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc
    25801 aaatgtcggg tggacaatcc gcaaagcaac caggtagcgt tgcgcaatgg ttttatcctt
    25861 gaaggttgcc ttaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac
    25921 gcgcgtatta tcgattcaca ataaccctgc cagcagcgtt cgcgtaatgc gctcctcgcc
    25981 gttaatgact tttggaccac ggacatgaat cgtgtcgcca tcaaaggctt caacgacggc
    26041 gtggtcagtt aactccacat ggttttcaat tatcaccgca cctgtaatac ggctctcacc
    26101 ttgtatgaca acgtgctcgt cgagcagaat cggtccgcca cataccacgg catttccgcc
    26161 aatcaagaca tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg
    26221 cgaactgtaa tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg
    26281 accgtagact ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaacgct
    26341 ggcaaaatca aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc
    26401 ataaatttgt gcctggtgga caatacgcga ggcgctaacc ctcgcgcgat cataaatttg
    26461 tagcaggagt tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc
    26521 aattaaggcg tgaccaaata ttctacattg accgcaaact aatgagtcac gaatggtgac
    26581 actgtcactt atatatgcac cctggctgat ttcactatta tcgatccaga cattatccgt
    26641 tgcgtaaacc tctccccaca gcacgctggt gccggtgata cgcgtattcc cggaaatcac
    26701 cgttccgccg aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa
    26761 caccatttcg cgatcgatcc agccgccagc ggttcctgct atcacatcgt taaaatcgct
    26821 aatggcgata atctgccgta ataacacatt tttttagtgc catcttcctg ataactaaag
    26881 gctcgctgtt cttcacttag acgatatttg cgcatagcgt tttcccacag gtgacttact
    26941 ataaccgtag caaattctgc ggctctggct atgctcgaga aaattccata aaatgcattt
    27001 caaatatact ttataaatta aacaaaatga gtaagaagat gcagagcgat aaagtgctca
    27061 atttgccggc aggctacttt ggtattgtgt tggggacgat agggatggga tttgcctggc
    27121 gctatgccag ccaggtttgg caggtcagcc actggttggg ggatgggctg gtgattctgg
    27181 cgatgatcat ctggggatta ttgactggcg catttattac ccgactcata cgctttccgc
    27241 atagcgtgct ggcagaagtg cgccatccag tgctgagcag ttttgtgagt ttgtttccgg
    27301 caacgacgat gctggtggcg attggttttg ttccgtggtt tcgcccactg gcggtgtgcc
    27361 tgttcagttt tggtgtcgtg gttcagttgg cttatgccgc ctggcaaact gcgggattat
    27421 ggcgcggatc tcaccctgaa gaagctacca cgcctggact gtatctgccg acagttgcca
    27481 acaactttat cagcgcaatg gcctgtggtg cgttggacta caccgacgcc ggtctggtgt
    27541 ttttaggcgc aggcgttttc tcatggctaa gcctggaacc ggtgatcttg cagcgtctgc
    27601 gcagttcggg agaattaccc acgacactgc ggacatcact cggcattcag ctcgctcctg
    27661 cgctggtggc ctgtagtgcc tggctgagcg tcaacggcgg cgagggtgac acgctggcga
    27721 aaatgctttt cggttatgga ctgctgcaac tgctgtttat gctacgtctg atgccatggt
    27781 atctctccca gccatttaat gcttcattct ggagtttctc gttcggtgta tctgcactgg
    27841 caaccaccgg tttgcatctg gggagtggca gcgataatgg atttttccat acgctggcgg
    27901 tgccgctgtt tatctttacc aattttatta ttgcaatact gctcatccgt acttttgcgc
    27961 ttctgatgca gggaaaattg ttagtcagaa ccgagcgcgc cgttttaatg aaagcagagg
    28021 acaaagaatg atcattcgtg atgaaaacta ttttactgat aaatatgaat taacccgcac
    28081 acactctgaa gtgctggaag cggtgaaagt ggttaaaccg ggtaaaacgc tggatctggg
    28141 ctgtggcaat ggtcgtaaca gtctttacct ggcagccaat ggttatgatg ttgacgcatg
    28201 ggataaaaat gccatgagta tcgccaacgt cgagcgcatt aagtccattg aaaatctgga
    28261 taatttacac acccgggtcg ttgatctgaa taacctcaca tttgatggac agtacgattt
    28321 tattctttct actgtggtgc tgatgttcct tgaggctaaa accatcccag ggctgattgc
    28381 caatatgcaa cgttgcacta aacctggcgg ttacaacctg attgtggcgg cgatggatac
    28441 cgctgattat ccatgtaccg tcggcttccc gtttgccttc aaagaggggg aattacgtcg
    28501 atattacgaa ggctgggaga tggtgaaata caatgaagac gtcggcgaac tgcaccgcac
    28561 cgacgccaac ggtaatcgta ttaaactgcg tttcgccacg atgctggcac gtaaaaaatg
    28621 atccggtaat aacaaagcgc gtgaaaattc cccacgctga gatggtttac tgttcttctt
    28681 ttcggtaagc atatttttta tcgaagggat gtgaaattaa tcacagtagt cgaagttttt
    28741 tagccgctta acttactgaa atttaactac tgatgattga cttagcccct ttttcggcat
    28801 tgactatgtc gtctgaaaag gggctaaaaa atttattttc accaacactt tttttgccac
    28861 aacacgaagc ggcgcttttt gctataactt agaaagtaat ataatcatct caggaaacta
    28921 ttcatgcgta ccacatcatt tgcgaaagtt gcagctttat gcggcttgtt ggctctgtct
    28981 ggttgtgctt ctaaaatcac ccagccagat aaatattctg gttttttaaa caattactct
    29041 gatttaaaag aaacaacctc ggcaacaggt aaacctgttt tacgttgggt agatccgagt
    29101 tttgatcaaa gcaaatatga cagcatcgtc tggaacccaa tcacttatta tccggtaccg
    29161 aaaccttcta cccaggtagg gcagaaagtt ctggataaaa ttttgaacta taccaacacc
    29221 gaaatgaaag aagcgatagc gcagcgtaaa ccactggtta ccaccgctgg gccgcgtagt
    29281 ctgattttcc gtggggccat taccggtgta gataccagta aagaagggct gcaattctat
    29341 gaagtggttc ctgttgcatt agtggttgcg gggacgcaaa tggctacagg ccaccgtacc
    29401 atggatactc gcttgtattt tgaaggtgag ctgattgatg cagcgactaa taaaccggtt
    29461 atcaaggtcg ttcgtcaggg cgaaggtaaa gacctgaata acgaaagtac gccaatggct
    29521 ttcgaaaata ttaaacaagt tattgatgac atggcgaccg atgccaccat gtttgacgtt
    29581 aacaaaaagt agttccagac gcgccatcgt tagatggcgc tttttattat cggtttctgt
    29641 tagcgattaa attcaacacc acgtaacaaa cgcctccggc aattaatccc caaaacgcag
    29701 aaccaatccc gaacagcgtc aatccacttg ccgttaccag aaatgccacc accgccgcgt
    29761 cgcgctcacg ctcattatgc agcgcctgat acaaactgcc gccaatggta ctcaacagcg
    29821 caagacctgc cagcatctgg atccaactta cgggcagggc agccatcatc ccggtaatgg
    29881 cactaccaaa cagacctgcg atcaaataaa aaataccagc aacggcagcg gccagccaac
    29941 gttgatcttt atccggatgc gcttccgggc tttggcaaat agccgcggtg attgccgcaa
    30001 taccgacgga ataaacgccg aaaggggaaa aaacaagcgc cagcaatcca gtaaacacaa
    30061 tcaacggcga aacaggagcc gaatacccgg ctgctttcat tgctgcgata cccggtgcgt
    30121 tttgcgatgc catcgtcacc agaaaaaggg ggagtgcaac gctcagactg tgagcaaacg
    30181 aaaaatcagg gggaatataa gtggggagaa cgggtttaaa gacaacatca gttgtgacaa
    30241 cgtcaccttg cgcgataacg atcacgatcc caataatcat cgcggcaatt accgcatagc
    30301 gcggcgcaac ggccctggtt gccagccata ccagcaacat actgccacac aacgtaaatt
    30361 gaccatccag actggcaaac gcctgtaaac caaaacgtaa taaaatcccg gcaagcattg
    30421 ccgccgcaag cgagtgcgga ataatgcgca tcagacgagc aaagagtccc gttatgccgc
    30481 agaggactat taacacgttg gtgacaataa aaacgccgat ggcttcgtta agtgttagtc
    30541 cctgtaatcc ggtgaccaac aaagccgcgc caggcgttga ccatgcggtg agaacaggta
    30601 cgcgatacca taatgtcaga gtcagcgtac tgacgcccat tgccagcccc agcgccgtca
    30661 tccagccaga gatttgtgca gtggtggctc cggcgacaat cgctgcttgc cagattattg
    30721 ccgctgaact ggcgtagcca attaataccg ccagaaaccc cgccagtagc gtgggtggag
    30781 gaatagaaaa cagacgcata gtcacctcgt acgttataac gtacggctaa cgtagcattt
    30841 gtccgttata acgcacaagt gataaacttc cgttttgccg gaggagtcgc atggaaaatc
    30901 tcgctcgctt tttatccacc acacttaaac aactacgtca gcagcgcggc tggagtcttt
    30961 cgcgactggc agaagcgacg ggcgtttcta aagcgatgtt agggcagatt gagcgtaatg
    31021 aatccagccc gacggtcgcg acattatgga aaattgccac cgggttgaac gtgccatttt
    31081 caacatttat ttctccgccg cagtccgcta cgccttcagt ttatgatcca caacggcagg
    31141 cgatggtgat cacttcgctg tttccttatg atccacaact ctgctttgaa cacttctcga
    31201 ttcagatggc acccggtgca ataagcgaat cgacgcccca tgaaaagggg gtaatcgaac
    31261 atgtcttcgt cattgatgga caacttgatc tgtgtgttga tggtgaatgg cagagcctta
    31321 attgtgggga aggcgttcga tttgctgcag acgtcacgca tgtctatcgt aatggtgggg
    31381 agcaaaccgt acattttcat tccctcatcc attacccgcg cagttaagcg ggaaaactat
    31441 ttcgcaacgt cgcgcttgtg gctaaaatag ccgccatttt tcagctactg gataagaaag
    31501 tgaccgtatc ttctcatcgt cttgaactgt taagcccggc acgcgatgcc gccattgccc
    31561 gcgaagctat tttgcacggt gccgatgctg tttatatcgg cggccctggt tttggtgccc
    31621 gtcataatgc cagtaatagc ctgaaagata ttgccgagtt ggtgccgttt gcccatcgtt
    31681 atggtgcaaa aattttcgtc acgcttaaca ccattttgca tgatgatgag ctggaacccg
    31741 cgcaacggct gattactgac ctctaccaga ccggtgtcga tgcgctgatt gttcaggata
    31801 tggggattct ggaacttgat attccgccga ttgaactgca cgccagtacg cagtgcgata
    31861 ttcgtacagt agaaaaagcg aagttcctct ctgatgttgg cttcacgcag attgtgctgg
    31921 cgcgagagct gaatcttgag cagatccggg cgattcacca ggctacggac gcgaccattg
    31981 aattctttat tcatggcgca ttgtgcgtgg cctattcggg tcagtgctac atttctcatg
    32041 cgcaaacagg gcgtagcgcc aaccgtggcg attgctcgca ggcgtgccgt ttgccataca
    32101 cattgaaaga cgatcagggg cgtgtggttt cctatgaaaa acatctgctg tcgatgaaag
    32161 ataacgatca gactgccaac ctcggcgcgc tgattgatgc tggcgtacgc tccttcaaga
    32221 ttgaagggcg ttacaaagat atgagctacg tgaagaatat caccgcccat tatcgtcaga
    32281 tgctggatgc cattattgaa gaacgtggcg atctggcgcg cgcttcatca ggtcgtactg
    32341 aacatttctt tgttccatcg acggaaaaga ctttccaccg tggtagcaca gattattttg
    32401 tgaatgcccg taaaggcgat attggcgcgt tcgattcgcc gaaatttatc ggcctgccgg
    32461 tgggcgaagt attgaaagta gcgaaagatc atctcgatgt tgccgttacc gagccactgg
    32521 caaatggcga tggcctgaac gtgatgatta aacgtgaagt cgtcggtttt cgtgctaata
    32581 cggtagagaa aaccggagaa aatcagtacc gcgtctggcc caatgaaatg ccagcagatt
    32641 tgcacaaaat tcgcccgcat cacccactaa accgaaatct tgatcataac tggcagcagg
    32701 cactgacaaa aacctccagc gaacgccggg tggcggtaga cattgaactg ggcggctggc
    32761 aggaacaact gattctgacc ctgaccagtg aagagggtgt cagcatcaca catacgctgg
    32821 acgggcagtt cgacgaagcc aataacgccg aaaaagcaat gaacaatctg aaggatggtc
    32881 tggcaaaact ggggcaaacc atctattacg cccgcaatgt gcaaattaat ttgccggggg
    32941 cgctgtttgt accaaacagt ctgttaaacc agttccgccg tgaagccgcc gatatgcttg
    33001 atgctgcgcg tcttgccagt taccagcgcg gcagccgtaa accggttgct gatcctgcgc
    33061 cggtttatcc gcaaacgcat ctgagtttcc tcgcgaacgt atacaaccag aaagcgcgtg
    33121 aattttatca tcgctatggt gtgcaactga ttgacgcggc gtatgaggca catgaagaga
    33181 agggcgaagt cccggtgatg atcaccaagc attgtctgcg ctttgccttc aatctgtgcc
    33241 cgaaacaggc gaaaggcaat atcaaaagct ggaaggcgac gccaatgcaa ctggttaacg
    33301 gcgatgaagt attaacgcta aagtttgatt gccgcccatg cgagatgcac gtcattggca
    33361 aaatcaaaaa tcacatactg aaaatgccgt taccgggaag cgtagtggca tccgtaagtc
    33421 cggatgatct gctgaaaaca ttgccgaagc gaaaagggta aaacgccagt tttcgggtta
    33481 ctcaccactc atcaaatctg catgatattg cctgccgggt aaggcgtcat gccgcatccg
    33541 gcaacaaatg actcaacgct gtcccgcttc tggtttgcgc gatttttgcc agtaaaaatg
    33601 ctcgcgcaaa ccttccgccg actcttccgc cacagcacgc aattcatcgc tgtccgcttc
    33661 atgacgcagc tgatgatcca cattctttac ccacataaat tcatgtcctt tgtgccctgc
    33721 catgagttgt cctgagaact gtaaccaggt gaatcatttg ataccaacga gggttttagt
    33781 gaaaggaggt gaaatcacct gtaagatact gaaataaaac aggtgatgtc tggatgagcg
    33841 cttaaagtct atttgatatc gattgtgcaa taattaatcg tttattctaa aatcggcaaa
    33901 acttttgata ctgcgatctc aactttttaa agcgtttaaa acttcattaa atctatatgg
    33961 caactaacat acgggccagc ctcgccaaac taacccgctt tagattctac cggatttgat
    34021 tgcctacttg tctcggtttt ttccaaaatc tctctgacgc aatcgtctgg catatctaca
    34081 atcatcaacg cagccaggcg cttcctgcct tctagttgta ataaatcctg gttttcttta
    34141 ttcagcagtg tcagaaccgt ctctacacct ttgcgagcaa ggagattggt taactgttct
    34201 cgttcgtctt tcgtaaggaa tgaaagcatc tgtgtaagct gatctccctt atcaccagaa
    34261 caaccaaaat taccataaat accaatatcg tttggtaatt cacccttgcc ctctttaagc
    34321 cactcaagac taacgccttc actttcagaa attcgtataa ggatactttc tctgggggct
    34381 ggttgtgttt gtcctccctt gaaatagctg tttaatgtgt tgatattgat tccccacgct
    34441 ctggcagctg cacttcttga agggtagcgc tttgtaagca ttccaatacg ttcaataaca
    34501 ggttcttttc cctgcgacat aaagatacct cttggtgctt ttctaaggaa aggtttcttt
    34561 ctatttatcc aattgaaaaa tgaggataaa agcaaaaatt agccaaaaca ccaaaaatac
    34621 cttgatttgg tatttttggt gatgcattat ttacccgtac agatatccgc acgaatatcc
    34681 gcgcgggtaa ctttttagaa tagaggaagt atgagtagaa gagaagtttc agatcacgat
    34741 tggccgcctg aaatcatcaa ggcgcgctta cacatggctg gcttatcact gcgctcttta
    34801 tctttaaagg ctggttacag cagggattcg ctgaaaagcg tattacgtac tccttgccga
    34861 ccgtatcagc aaataattgc tgatgctctt ggggtatcac ctgaagaaat ctggcccagt
    34921 cgataccagg ttaagagcta tatgagaaag gcgtcatgat atgtatgtca ttgcaaaaga
    34981 actgattggc gcgcccggaa tgcctgctac aacaaaaggt attcgccagg cattacaacg
    35041 ttacgtacaa gggaaaagct gttgttcccg tcgtcgctca ggctctaaag caactgaata
    35101 cagcatcgac tgtttacctg aagtgacgca gcaggcatta cgtgaacgct acgccctgca
    35161 actgatgacg caaaaagccg atgaatcacc ggctccggtg gtgacaaagg ccagacgctc
    35221 acctgccgtg gttgatgcag tggaggcata tcgcggatca ccccaactga tggtcgaacg
    35281 cctcaatgcc ctgactgaaa accaacgtaa agtggcagag gcacgaatag cgatcgtcag
    35341 cgaggtgctg aaaatcgcgc aacaacccgg ctttagttgc gctaaggcta tccggttcct
    35401 cgttaacagc ctggcacgat cacaactgga cgagcgcatt gtggcaatgg ttgagacggc
    35461 gaacgccaaa aagggaaaca gccgcgcgtt gagtgaaatc acgctgaaac gctggattgc
    35521 ggcctttaac aaggcacaga acgccgctga acgcctgctt ttactggcac cgggtaaacg
    35581 ccaggaaata aaagccgaag atattaactg gctgcccgaa tttctggcgc agtatcgcca
    35641 gtcaaacggc cgaccaatga ccgaggctta cgaggatttt gttgctgaat ggcagcaccg
    35701 gcacgctgat gagccttata tgctcgatat catgccctct tatgacacca ttcgccgcgc
    35761 aatgaagaaa ctgccggaag tggtgaaaca aaaaggccgg gtaactggca gtgaataccg
    35821 tcagcttgag ggattcacgc gccgcgactg gtccagaatg ccggtgaatt atgtctggat
    35881 tggtgacggt cacggcatga agctgaaatg cgcgcacccg gttcacgggc ggccattcgc
    35941 accggaagtg acctttgtta tcgacggtgg cacgcgcttt gtggtgggct ggagccttga
    36001 cctggctgaa aatgttttcg ccgtagccgg tgccatacag cacggtattc gccatcatgg
    36061 caaaccgttt ctgtattact cggataatgg ctccggggaa accgccgacg tcctggataa
    36121 ggagattgtg gggatactgc cacgactggg gattaatcac ccgacaggga ttgccggtaa
    36181 tccgcaggga cggggcatta tcgaacggct taaccgcaca ttaccgatgc gcatagcccg
    36241 taaataccgc acctatttcg ggaaaggagc agatcgcgag acgttacgta aaaccaaccg
    36301 cgatttacgc tcggcattca gtgccctgca acagggcaaa cggctgaacg cccggcagca
    36361 gtcagcgatg cgtgatttac cgtcctggtc tgaactgatt gatgccattc gtgacggtgt
    36421 tgagtggtac aacaaccggc cgcacgatga attacccatg aagccgaacg gtaaacatta
    36481 cagcccggcg gagttcagaa aaaaacgcct ggcggaagag gacacggaaa ttgaatggct
    36541 gtccgatgtt gaactgcggg atatgttccg gccgatggtg gagcgccctg taagacgctg
    36601 tgaaatacgc tggctgaata atatttacta cgcgcccgag ctgcgtgacg agcatggccg
    36661 taaggtactt atcagctatg acattcatga tgccgaacga attaccgtgc gccgcccgga
    36721 tggcagcgtg atttgcgagg cggtatggga cggcaataaa cgcgaagcct tccctgtcag
    36781 cgcggaatac tacaaacagc agcagcgcct taaaggcatg cgtaaacgcg cagaggaaaa
    36841 aatccgtgat gccgaggatg aggttgtcaa cgtgctggag cacaagccgc aggagccctg
    36901 gctggaaaac atataccgcc ctgtgggtaa tacggtgacc gttcagcaac cggtcgctga
    36961 cgatgaacct gatgaagaat acgagcgtaa tttccagcgg ggattgcaac tgctcgaagc
    37021 gaaattaaaa gaaagtgatc cgctggcctg aaataaaaaa ataacccgag gggtgactca
    37081 ggttatttga ggtttcagtc tcggagaatg ctgtctcaag cgaggtgaat aatatgaccg
    37141 atattaacga tgtaatcaag accattgatg aacttattga tggcggcgta ctgacgcagt
    37201 atgccatcgc cagagaggcg ggaatttccg acggcacatt atcggctttc cgtaagggaa
    37261 aatataaagg cgataacgct gctgtggctg cttccctgcg ctcctggtat gagaactgga
    37321 ataaacaaag cgcactgccg gaaccgccgc agtttgtgga aactcagaca gtccaggagc
    37381 tgcgcgcact gtttcaggcg gttcgcctga tgggctgtat taacgttatt gtgggcgtac
    37441 cgggtgtggg taaaacggcc actgcccgta attactgcca ggagcaacca aacacctgga
    37501 tgatcaccct gtcacccgcg cactccagcg tcacggagtg tctgctggag ctggccgatg
    37561 cgctggggat tgattacacc cgcgcgaaca aaggggcatt atcccgcgcc atccgccgtc
    37621 gcctgatggg aacgcgtgga ctggtgattg tggatgaggc ggatcatctt ggtattgacg
    37681 gtctggagca actccgggca attcaggacg ccacggggat cgggatggtg cttattggta
    37741 acccgcgcgg attgtttaaa ggtggacgcc gcgcctttga tgatttatcg cgcctgttca
    37801 gccgtcttgc ccgtacaaaa caacttcgca aggccaaaaa ggcggatgtg ctggccattg
    37861 ccatggcgtg ggggatcagt ggtgaggccg agctggccgt catgcaggct atcgctgaaa
    37921 agccgggagc gttacgcgtt ctgacacata cgcttaacca ggcgtggctc accgccagcg
    37981 gtgaaggcgc ggcgctgaca gaaaaacata ttaatgcggc ctttaaagag gtttatacca
    38041 accctgaatt actctcacag gtgtgattat ggcggtattt aatattcctg atatttacgg
    38101 acgcttttac ctggttaatt tcgataacgt gaaggtgatt tcactggccg aaaataaaga
    38161 atgtggcgat ttactttttg aatttaatga ccgcacacga atggtgatat ctgccggact
    38221 tgatcgcgaa ggtgcgacag aagtttacag cggaatatgc cgttctgttg gtgcgaaaca
    38281 agtcagctaa atgaggtgtt atatgaatat gcaatcctgc ggtaacaaaa tgaatttatt
    38341 cgactccctg aacagcgcgc gccgtctgac cgaacttgcc ggtgcggtac tggaacgcag
    38401 taagcgctat ccgcaacgtt ttgcactgaa aaccacgccg ccggtaggca acgtgcaggg
    38461 aaccggtgaa attgaaatca ccatacagac caacggcctg cgccgccgtg tgaaggccac
    38521 ccgcatcagc ggctgcacgg tttactggga ggtgtgaggt gaaaaaaaat ctcattgcat
    38581 gggcgtggtc gagtggtctt attgagtttg gttacgtcct gccggaaggt gcattgccga
    38641 tagttgccgg aaagcctgcc acggtacggc atgtgattga ggttatggcg cgtcatggac
    38701 gtgatgaaca ggagcagtta ctggttccgg ggataccgga agcggtgacg gaggaagaag
    38761 cctttaatgc catgattcgg ttctgccgtg aggttagacg ccgggtcagt tatccaaaca
    38821 gaacgaggac cagaggatga gtaaagtcgt acgcattatt ttcgaataca aggaagatgt
    38881 tatctgcaaa aactctgatg gcagcacgca cagaggggta agtctggata tacgttcaac
    38941 cggaataaag aagaaagata atggacccgc catgattttt ggggtggtta tgctggcaga
    39001 aagcagaaac ttcgcagagt ttgtggcaat gaaagccagt gcattcatga aagacagagg
    39061 catggcttcc ggggttatta acggtaatga atttaatcaa caggggtaat tccatgagca
    39121 aagtacgcgt tatttttgaa tttaatcatg tttcgcatga cgaaaagttg gcaggcaatg
    39181 actgtgttga agtgcatgaa aagattggag tggatgtgaa aacagaacgt gatacggata
    39241 acaggccgac gtcactctgt gacgtttatg caagtattct ccagtatcac agtcctgcaa
    39301 ttattcagtt tctctcagcg gaatttcagg catctgcaca gtcttttgga gcggatgcca
    39361 tcattaaacg ccaccgcgtg cataaagcat caggcacact gcaataagga aaaacaaaat
    39421 ggctaaacgc gttacaaaat taaaggccgc agccgaggcg gcaccgcaga cccgggaaga
    39481 agtcagccgt gatatccgca ccctgggaga tattcagcga gaggcgctgc gcctggaaac
    39541 ggcgatgaat gacgaagtgg cagaaatcac cgcccgttat acgccgcaga ttgaaaatct
    39601 taaaaagcaa atcaaagtgc tttttaaagg gattcaggac tggtgcaaaa ccaaccgtga
    39661 tgagctgacg aacggcggca aaaccaaaac agccaatctg accaccggaa cggtgtcatg
    39721 gcggctggga acgccatcat gcagcgtcag ccgtgatgtg gaaggtgtga ttgaaatgct
    39781 gcgccgtatg gggcttgagc gtttcatccg cacgaaagag gaagtgaaca aggaagccgt
    39841 cctggcggag ccggatgctg tgaaaggcat tgccggtatc aaggtgaata aaggcgctga
    39901 aagtttttat gtcgagcctt ttgaacagga cgccggactg aataaataac accacattaa
    39961 tcaattaaat atcacttcat tttaattatg gcgctcgcgt caggggactg cttgcgcctg
    40021 aaaacagaaa ataagggtta agaaatatga aatacgttta tatcgtcatt aataatgtgg
    40081 tggggaaata aatcatgatt gatgcaaaag tgcttgaagg ggttaaaaac tggctgtgta
    40141 tttatggccg cctgacctgc ggtgtactgg ctgaaaaaat gaatatgccg ccatcctcca
    40201 tggtttattt tctgcgtgat gcggttgatg caggcgtgct gacggaatgt aacggttttt
    40261 atgatattcc gcgcccccgc ccggtgcagc cggttcgtcg caaatgcagc caggaatctg
    40321 cggctgatga tgttcagtgg tgcggcttca gaaaatccct gccatggatt gaggggcatg
    40381 atattccgtc gatggcgtgg gaatttgctc agggcgttct gacctgtgaa accgtttatg
    40441 tggtggctga agttgatgag caggccatga aagaaggcgt gccccagttt gtgatggcgt
    40501 atatcgacat acgcctgggc gtcattatct gcggtttaag cggctggaat atcactgaac
    40561 atgttctgcg ctacctgatt gttgaccgga cagcagcgcc tgccgggata tctgcggagg
    40621 tggcgtaatg ttctttaaaa catcaaaccc ttccgcgctg gctgcgtggc aaaaatacca
    40681 gcaggactgc cagaaagtta aggatgaggc aaaacgcctt gaggccgtgc tgaatgttgc
    40741 gtgccggtcg gtatttgtat ccggtatcag tggattttac tttaagggac tgcgttttac
    40801 ggaggacaaa tatccttttc atcgcgactt atggcgaaaa ccgactgcgt cgaatggctg
    40861 gagctgcaca ccgcgcacat cacgtatccc taaaaccctg cgtgttgcct ctgatgaact
    40921 gaacagtctg tggcgtgaat attcgcccgt cacgtatgcc agaaccgatg cgctgttgtt
    40981 ctggttgggt attgatttct cggcaatcct gtttggccct gtgaagtggt tctgcgttga
    41041 cgatgtgatt taccttcagt gcggcgtaaa acccgcaaaa cagaaaatga ccgaaatact
    41101 gtctgatgag ttttatgctg ctgaaaagcg agtcaggggg tgatgcatga tgaaattaca
    41161 acccatggga ctaaaaggtc gcgcccccgc tcatgtccgt ccgtggacac ctgaagaaga
    41221 tgcgctgctg attgcgcttt attcatccac cccggttaag gatattgctg ccagaataaa
    41281 aagaactgtc tgggctgtat ataaccggac tggtgtattg cgcagttcat acccggagtt
    41341 actgaaatat aaacacccaa gatttacacc tgatgaagat aagtttattc gaaaaaatgc
    41401 cagaacaatg acctgccagc aaatgggaga atatctcgga cgtaataaag attctgtcag
    41461 atgtcgggca ggaatgattg gtgctggatt aacaaagtgc ggagagttac gccccggcac
    41521 gcgcatatct gatgatgatg tacgtcttat acgtgcgctg cgtgattccg attacccacg
    41581 ccgtctgtca ttccgggaaa ttggcgaaaa gtttggaata tctgaacatt ctgctcacgc
    41641 agtttattac cgtcgccgga ctgccgagga cgctgtatta cgggagttaa cgccatgata
    41701 acgaccttat ttgttgaatc agatgaacct cttgtgtgtg ccgccggaat gccgctctgt
    41761 ggcggaacgc tgaccggcgt ttatttcggg gatttgcgcg gttatccctg gcattcactg
    41821 gatgacgcat ttccgcctga tatggaggct gtcgtgctga ttgttcagta tggtgaccaa
    41881 caggaactgc gcatcggcca tatggggtat gaaggctttt ttattgatga agaaaccgga
    41941 gcctgccttg aagatgagga cggacaggtg acgcactggt gccatctttc ggccttgccg
    42001 gaattacagg ggatacataa tggatgagca aattgttgaa tgtcccacct gtggaaatga
    42061 ggacccggag tatctgaaag agtgcccaca ttgtggtgag ataaaatgta accactgcga
    42121 tatgggcgac gacactgcat gtataaattg tgaggatgaa taatatggaa aaagaaatca
    42181 aatttgcgcc taaatctatc gacgaagaac tggctaaaat cggcatgctt gaacgtatgc
    42241 gggacattat cgaatatgcg ataaaagaaa atcttgcagc cagagaagcc cttttgataa
    42301 tggagcggga gattaacctg attaaggatg ctgtatctct ggataataaa atagcccgcg
    42361 aggaatacgt tcgccgtagg ctcggtgttg atggttcagc aattcttaca tctgaacatt
    42421 atgcaaaaat ttttaatctt tttcagcggt aatccccata caggcccgga gattgtcgaa
    42481 atgccagtcg gtattaatat atgagccatc ctggatatct tctgcatttt ccggggtaga
    42541 gtttttttga gcgagattga taatatgtat tgcctcctca cgggaaatca gccccttatc
    42601 gaacagggtt accataagat ttccagccat tgtgaatgcg gcaacatcaa gtgcattttt
    42661 tatatctgac atagattttt ccttttatgt taattgacat ggcggtgcgg taacaccgcc
    42721 cattttttat ggagccacga taatgaatcg cgcttccctg attaccttaa tacatgtcgc
    42781 aaaacgcgat ctacagcttg accatgatac ctatacatcc gcgctgctgg cagccaccgg
    42841 caaatccagt tgccgtgata tgtcacccgg tgagttatcc cgcgtgctgg atgttttcaa
    42901 aaaacgcggt tttaaagtgc gtcagaagcc ggttaaccgg gccttaaaac cgggtacggt
    42961 gaccgccaaa attcgcgcca tctggaaagt gatgcaccgg cagggattta tctctgatgg
    43021 cggggaaacg gcccttaacc gctgggtgaa atcgcagacg gccgcgcaga acgacggcga
    43081 aggtgtggct aactggcagt ggctggagca acaacccgcg ctggcctctg atgtgctgga
    43141 gcgtctcaaa cgctggcacc gccgcaaaat gctggcagcc ctggatatgt cggaacgtgc
    43201 agtgatgagt tatgagcgtg tttgcaggct gtatgaaaaa tcattccccc gttaacccca
    43261 aatcccgcca cagcgcggga tttttatttt aaacttgccc tgtgtaatga tgaccggagg
    43321 cgggtatggc tgaaacacag atgagcatgt ttggtgacag cgaacagctg cacgcgctga
    43381 ttgaccgtct ggatgatatt cctgatgatg agctgaaaaa gaactggccc ggcacgctgc
    43441 gggacctggt tgatgtgatc agtgctgaac tgcaccgtca ggggattgaa cctgctcagg
    43501 cgggaatgct tgcccgtaag gttgcagccg cacaggccgg gtacatgggc gggcgtggtt
    43561 attatctccc ggtcggtgaa tcccttttca ccgaactgcg caataatgaa atttttttcc
    43621 gctggagcca gggcgaaaaa attgaaaccc tgcgccgcca ctaccagatg tcagagacgc
    43681 agatttactc cgttatccgc aaccagcgtc gcctgcatca ggaacgcacg cagccacggc
    43741 ttttctgata ttccgcaaat cctgcccact ttttcccacg gttacgctga ctcagagaac
    43801 accatgagac agcgtaacaa tgccaaaact ccccgcaccc ctgcgtaaaa agctgattgc
    43861 ccttgttctg gccggagccg ggacgttcac cattgccacg cactacaccg gctactggga
    43921 agggaaagaa aacaccacgt acattgatcc caccggcacc cccaccatct gttacggcca
    43981 taccggcccg gatgtgaaac cggggatgac aaaaactgat gaagagtgtc tggagctgct
    44041 ggaaaaggac atgaaatggg cctttgcggc tatcgatcgt catgttcagg tgccgcttac
    44101 ccgtggtcag acggtggcgc tggcctcctg gattttctgg gccggcgaaa cgaactttcg
    44161 caactccacg ctcctgcgcc ttatcaatgc cgggcagatg cccgcgtcct gtaagcagta
    44221 tatccgctgg atttattcaa agggggtaaa actccccggt cttgaggccc gccgttcggc
    44281 ggatgaatgg ttatgtcgtt acgatttgcc gaaagtctga accgcttctg gcgaccgctc
    44341 atgattgcgc tgctgtgtgc cgttgtgctg ctgcggggtg tgctgtgtct gcggtgaaca
    44401 atcccttacc ggccctgatt gccggaggct gtatggcggc gctgggggtg atgagcgtgc
    44461 tggctgccgt ggtatgggga atgcaccaga agacgcagcg ccttgaggac aataatcatg
    44521 tgcttgtgcg tgaacgggac gaagcccgtc tggtgctggc aaaccagcaa cgcaccctgc
    44581 aactcatttc acaaatcagc aaggcggcca cgaatgaaaa acagcagaac atgcagcaca
    44641 gcgaggaaca gcagagcatt gtccgccggt cactggcaac ggtgcctgct gccgctgtgc
    44701 ctgtgcctga tgatgtggct gaccgggtgc ggcgggccgt ctgtgaaatt cgtccctgtg
    44761 ccgccggtgc cgatccccgc tgaatggctg gctgactgcc tggtccctcc cgcgccgcag
    44821 ccgttcacgt ttggtgcatc ggtcacttac aacctgcaac tgctggcggt gattaaaaac
    44881 tgcaacgtgg acaaggccag catccgccgt ctggagacac agcgacagca tgaatttact
    44941 gatatggccg gaacgactgc tgttccggca ggaaagacga agtaaggaaa aaggtatgga
    45001 tgattcagat tgtgctcagg cagtcatgga gcgtgcaaca gaacgcgccc tttgtgaccg
    45061 gctgacacgt aagcgccggg tggcggagac accgggcagg cgtgtatgcg cggactgcgg
    45121 gggtgacatt ccggctgccc gtctggttgc cgtgccggat gccatccgtt gtgtgaactg
    45181 ccagaacatc atggaggccg gtcatgtggg tcagcatcgt taaagattat gtcgtgccga
    45241 tcctgtcggc aacggccaca gccgggggga tcttcatggc gctgatgcgc aaaacctttg
    45301 tcccccgcga ggcctttgaa aaactctccg accgggttga gcgggtggag agccggattt
    45361 ccggcctgcc cacgcagcag gaggtgaaca gactcaatat cgaaatcacc accctgcgcg
    45421 gtgagcttaa ggccaccaat gccaccctga aatccgtctc ccatcagaac gaactgctgc
    45481 tggagcaggc tgtaaggaaa aaacacaatg agtgatttca ttactgaaga tcagcgtctt
    45541 gtcattctgc ggtcgctggc ggattacaac ggcgaactgg gcgaatccgt gcttcaggac
    45601 tgcctggatg attacggcca ccgcgtgtcc cgcgacaccg ttcacaccca cattgcctgg
    45661 cttgctgagc agggactggt gcgtaagcgc acgcttgtta acggttattt catcgctgaa
    45721 ctgaccggcc ggggtcagga tgtggcggaa ggtcgtgcca ccgttccggg tgtgaagaaa
    45781 ccacgcgcaa gggggtaacg atggataagc cgacgcgtgg ccgtgtacgc aaggtggatt
    45841 tgctccccga cagcatccgt aagccgctgc tggaaatgct gcgtgaaaaa cgcctgacgc
    45901 aggtccagat ccgcgaggaa atcaaccgtc tgatccgtga ggcaggcctg ccggaagagc
    45961 agcagctttc accggcggcc atcagccggg aagcctcccg taatgagctg attgcccgta
    46021 atctgcgtga tttacgcgag cagacaaaag ccatgatggc cgaactgggg gataagccga
    46081 ccggtgaaac cacggcactg attctggaga tgtcccgtgc cctgatgtac cgccgtctgc
    46141 gtgccgccac cgaatccctg aacagcgaca gcgatgtgga tatgcgcctt attaaggaca
    46201 tcctcctgtc ggcacagcgt gcggaaagtg ccgccgagcg cagcattaag cgtgaaaaag
    46261 aaatccgggc cgcatttgct gaagagatgg cgaacgccgt caccgacgag ttgcgcggcg
    46321 tggatggcat gagcgagcag atagaattcc gtatcaggca gatgctgctt ggtaaggcgt
    46381 aggaggtgaa tcgtggtaac agataaccgc tcactgatga gcagtgctca tcagaaggca
    46441 ttcagtcttg caacacagtt acctcctgaa accataaaag cgtgtcgtga gttgactgaa
    46501 ctcagcattg ccggggaaat atccctggaa caactgcatt cgctgctggc aggggctgtc
    46561 actgatgagt tgagtggcgt ggacgtgatg actgaagagc ttgagttccg aatcaaaagc
    46621 attctgacag gtaaagcctg atgaacaaag ccaaagaaaa gtctccttca ctgatacaaa
    46681 tgacacctcc ccgcaaaatt gatctgtccg gtgaaaaagc cctgctgggc gtggatgtgc
    46741 cggacagcct tgatttgccc ggtgatatgc cggtgtttct ggattatcag gcccgctggt
    46801 ttgcagatga aagtcaggtc tgcatcgcgg agaaatcccg acgcaccggg cttacctggg
    46861 cggaagccgg gcgtaacgtc attaccgccg caaagcctaa gcgtcgtggt ggccgtaatg
    46921 tgttttatgt ggggtcaaaa caggagatgg cgctggaata tatttctgcc tgcgcgctgt
    46981 tctcccgtgc ctttaaccag ctggcagatg ctgatgtgta tgagcagaca ttctgggatc
    47041 gggataaaaa agaagaaatt ctgacctaca tgatccgctt tccgaacagt ggattcaaaa
    47101 ttcaggcatt gtcttcccgt ccgtcaaacc tgcgcggtct tcagggggat gtggtgattg
    47161 atgaagccgc gttccacgag tcactggatg agcttctcaa ggcggcagtg gcgcttacca
    47221 tgtggggcgc acgcgtgcgc attatttcca cgcataacgg cgtcgataat ctgtttaatc
    47281 agtacattca ggaagcccgc gaggggcgca aggattacag cgtccaccgc ataaccctgg
    47341 atgatgccat tgcggacggg ctgtaccgtc gtatctgtta cgtcaccgat caggaatggt
    47401 caccggaaag cgagcagaaa tggcgtgatg atctctacaa aaacgccccg acccgtgagg
    47461 atgccgacga ggaatacggc tgtatcccga aaaaatccgg cggtgcctat attcctcacg
    47521 cgcttattga aatggcgatg attcgtgaca tcccgattct gacgtttgaa gccccggacg
    47581 acttcatcag ccgtgccgca tggctgcgtg aatcggaagt tttaacctgg tgtgaagaac
    47641 atttaaaacc gcttacagag aaattaaatc cccgttcccg cttcagcttc ggggaagact
    47701 ttgcccgcac cggtgacctg tcctgctttg tgctgctgga aatcaccgaa ctcctggcaa
    47761 agcgcgaggt gtttcgcgtg gagctgcgca acctgccgta tgcccagcag gaacaggtga
    47821 tgatgtacat cctgacccgc gttccggcgc tggtcggtgc ggcgttcgac gccaccggta
    47881 acggcggcta tctggcagag gccgcactgc tggcctttgg cccggacatc atcgactgcg
    47941 tgatgttgtc gccgaagtgg tacggcgagt ggatgccaaa actgaaagcc gagtttgaag
    48001 atcagaatat cctcgtcgcc cgccatcaga ccacgcttga tgatttgcgc catgtgaagg
    48061 tggtgaatgg tatcccgcaa atcgacaagg ggcgcacgaa ggaccagaac gcaacggctg
    48121 cgaacgcccg ccgccatggt gattttgccg tggcactctg tatggcaaac cgggcgtcat
    48181 acatggaggg tttcatcctg gatgaatcag cctgtcaggc gctgccggag cggtcgcggg
    48241 caatggaggg cggttatcgt gatgacgatg aggcatatca tgaatttgat cgggggtgct
    48301 ggtagtggga cgcataattg atcttgacgg aaaacctttc tcctttgacc cggagatgca
    48361 aagtaccgcg ctggatattc cgcagattgc cagccgttat gttgagcatc ccgcctcggg
    48421 tatcaccccg aaccgggcgg cgcagtgcct tcgcggggct gaacgtggcg atctgattgc
    48481 ccagtccgat ctggcggctg acattgaaga aaaggacacc cacctttttg cggagctggg
    48541 caagcgacgt cttgccattc agggggtgcc atggagcatt gagccgccac cgaacgccag
    48601 cgcgaatgag aaaaaggacg cggaaatgct cgacgaatat ctgcattccg ccgactggtt
    48661 tgatgccatg ttatttgatg ccaccgatgc catcctgaag ggctattcct gcatggagat
    48721 tgagcacggg atgctcggta agatgcacat catccgcgcc atccgatggc gtgacagcgg
    48781 gcatttctgc cttaacccgg atgatttgag cgaactgcgg ctgcgtgacg gcagccatgc
    48841 cggggtggcg tttcagccct ttggctggat agtgcatcag tcgcgttcac gcaccggcta
    48901 cggtggtgcg acggggcttg tcagaacgct tatctggccg ttcattttca aaaactattc
    48961 cgtgcgcgat ctggcggaat ttctggaggt gtacggcctg ccaatgaagg tcgggaaata
    49021 cccgtccggg gcaacaccgg agcagaaaag tgccctgatg cgggcggtga tggatatcgg
    49081 gcgacgtaca ggcgggatca tcccggccgg gatgtcgctg gagtttcagg cggcagcgaa
    49141 cggtcaggcc gatccgtttg aaaccatgat ttcgtggggt gagcgttcca tctccaaagc
    49201 aatactcggc ggcacgctga ccacagaagc cggagacaaa ggcgcgcgct cgctgggtga
    49261 ggtgcataac gaggtgcgcc gggaaatcag ggattctgat ttacgccagc tggctgcaac
    49321 cctgaaccgc gatctggtgt atccgctgta tgccctgaac accgcccacg ccattgatat
    49381 ccgtcgcctg ccgcgtatct gtttccagac caaagaaccg ggggatatca ccaaaatagc
    49441 tagtgcggtg atgcaactca gtgcggggat ggatattcct gatccctggg tacgggatca
    49501 gaccggtatt ccacagccca cgcccggtga agccatattc cgtgtccgtc agagcagtaa
    49561 tgaaccggcc atgcctgaca gggaaacgct accggaaaaa caggaaaaga cagaacagac
    49621 ggcgctttca gcccgactgc cggaagcgaa aaacacgccg cgtgatgaac tggacgacat
    49681 gggggacgcg gtgcctgccc gccgcttaca ggaggccatc gatccggtgc tggagccggt
    49741 cattgatgtc atcagaacgc gggggctggc ggaggcactg gcagacctgc ctgcccttta
    49801 ccgtgaaatg gatgattccc gcctgatgac gctgctcagt gatgccatgt ttgctgcgga
    49861 aatgaagggg atgctggatg gctcagggga ttgatttagg ttatgccgcc acgcttccct
    49921 caaaagagac ggtggcatac ttccgcgcca aaggggcgca tatcagctgg aactggttcg
    49981 aaacagacgc ggatgttcat gcccgctcat tcacggcagc aaaaacggca cggctggatg
    50041 tggtaaccac attacaggcc gaagtgcagc gggccattga tgaggggatt tcacagaaag
    50101 aatttatccg cacactgacg ccacgcctgc aaaagctggg atggtgggga aagcagattg
    50161 tggtggacag cgacggtaac gcggaagaag tgcagcaggg cagtccccgt cgtctggcgc
    50221 tgatttacaa cgtgaacacc cgtgtggctt acaatgccgg gcgttacacg cagatgatga
    50281 acaacacgga cacgcatccg ttctggcagt atgtggcggt catggacagc cgtacccgcc
    50341 cgtcgcattc cgcccttaac ggtctggtat tccgctatga cgatccgttc tggaaaacac
    50401 actacccgcc caatggctgg aactgccgct gccgtgtgcg gccattgtct caggcccgtc
    50461 tggatgcaat ggggttatcc gtttcatccg gtgaggatca tctctccacc cgcaatgttg
    50521 aggctggcgt ggataaacag accggagaag tcagagaaat gccggtgacc acatattcag
    50581 atggcgccag aaccatgaca ccggatgtgg gctggtcata taaccccggc tcggcagcgt
    50641 tcggcacaga ccaggcgctg atccgcaaac tgatcgaggt gaaaagcccg gcgttacggg
    50701 aaatggtggt tcaggagatg aacaacagcc cggagcggca gctggcgttc cgcatctggg
    50761 caaaaaacat catgcagacc cggcgaggtg gtaacgatat ccgcacgctg ggctttatga
    50821 ctgaaagcat tgcgcaggca gtggaaagcc ggacgggaac gccaccggcc cgcctgctgg
    50881 cgatgagcgg taaaaatgtg ctccatgcgg acagtgtgaa acatcagaat gacggcatcg
    50941 ctctgacgcc ggaggacttc gggcgcttac cggcaatgct ggcaaggcca aaagcggtac
    51001 tgtgggacaa acggcacaac aatctgatgt atatcgtgga aaatcaggat ggcagtgtgc
    51061 agattgccgt taatgcaccg tacagtctga aacgtcagcc cgacaaactg gatgtgattg
    51121 tgaatgctta ccgggttatc aatatggata agctgaaatc ggatatccgg ggcggaatgc
    51181 ttgaggtgct ggaaggacaa attgattaac ggtcagtggc gggggtcgaa cccgcataca
    51241 tgatccaccc ggaggcaggc accgacttta ccagttaagc gtacactgac cgcctgttta
    51301 ttttatattc atcctttatg cggaggcaat atgtcatcca ttgatgcagc tgtcgttgtt
    51361 gacgttacgc gtctccagcg agtgtttgcc cggcttcagt ttgtgggcgg cggaaaagac
    51421 ctggcccgca gtgtggcggc gagtcttctg tcatcgtcag aaatggcgtt tgagcaggaa
    51481 aaagagccgg atggtgaacg ctggcatgac tggtcagatc cttaccgtga gtggcgtacc
    51541 cgtaagggat acacgcccgg caaaatcctg acgctgaacg gcgatctggc ccgccgcctg
    51601 accacggatt atggcgatac ctgggcgctg attggatcaa atgaacctta tgcagccatt
    51661 catcagtggg gtggcctgcc tggtatgcca ccgggaccgg cggccattgg cgcacgtccg
    51721 tatatgggct ttgatcaggt ggcagaacag gagatcatgg acgaaatcag aaaacgcttt
    51781 aaaaaggcca cagaaacgcc gtaatggttt aaggtatgca aacgtattac cttaccccct
    51841 tcaggcgcgt gtcgtgattt tctaacctgt atttaacggg ctttaaaatc tgcgtgaagc
    51901 gcctttctca ttccccccct gtcagcactt tctgaagtcc cgcaaaacca gccccaaaaa
    51961 tacccggctt atgctgccgg tatgaagaca gaaaatcacc caaaagacgc aaatcgcccc
    52021 tccggtttag ccgtgctttc agcttcgctg actgcatccg gcgacggatg gtgtcagtta
    52081 ctgccagccg gtcgggtaaa ggcccgtgac ggtcgcccgg aaaaaccgac tgaaggctgg
    52141 ctgattaacg aatccgcctg taaccggatg aaagccgggc tttccgccct gcatcagccg
    52201 ttactgattg attatgacca tcactccgtg aatgcgcaga aaaacggatt caaggctatt
    52261 gctgcaggat gggttaagcc ggaaaacatc gaatggcgtg agggtcaggg gattttcatt
    52321 aaacctgaat ggacaccaca ggcacaaaaa catattgatg accgtgaata tgcctatctg
    52381 tctgcatata tgcattattt cgctgacacc ggggaaccct acctgttgcg catggcttcc
    52441 ctgacgaatg atccgggtat tacgggtatg aaccctgtcg cggcactcag tgctgatgat
    52501 ctctatgtcg tttccccttc tcaggagcaa tcccccatga atgagcaatt acgccagttg
    52561 ctgacggcgc tcggtctgac cgtggctgac ggtgacgaat ttacaccgga actgggcacg
    52621 gcggcgctgt ctgcccttac cgggatcaaa acccgtgcgg atgcgcacga caccctgaaa
    52681 acgcaggttg ccagcctgtc ggcagaactg gaaaccgcaa aaggcacacc aactggcggc
    52741 actattgatc tgacgaaata tgtgcccgtt gagacgtata acgccctgcg taccgaatat
    52801 gccgcgctgt cggcacaaca cggcagcacc acgctggagc agttgctgga taaggccgag
    52861 tccgaaggac gcattttcaa aagcgaacgc ggatacatgg aaggactcgg acaacagatt
    52921 ggcgtcgcgg cactttctgc acagcttgac gcccgccagc cggttgcggc tctgaccact
    52981 cttcagaccg acaccgtaac cgtgccggat aaaaagaccg ccaccgctgt gctgtcggct
    53041 gaagatatcg ccgccgcacg tctgttgggt aaaaccgaag ccgagttcct gaaaatgaaa
    53101 gaggaaatgc aataatgcct accccgatta caccggcgat gatcaccgcc ctgatgacgg
    53161 gctaccgttc tgattttcag gccgggatgt ccatggctcc gtcgcagtac cagaaaattg
    53221 cgatgaccgt gccgtccacc tcgaaatcca acacctacgg ctggctgggg cagttcccgc
    53281 agttccgtga gtggatcggc tcccgcgtca tcgagaagat gaaagcctat ggctacgcca
    53341 tcgtgaacaa aacctttgaa ggcaccgttg ccattagccg cgatgacttt gaagacgaca
    53401 atctcggcat ctactccccg ttattccagg aaatgggtcg tgccgctgcg gcacaaccgg
    53461 atgaactggt ctttgctgca ctgcgtgatg gtctgaatgc ggcctgctat gacggccaga
    53521 acttctttga taccgaacac cctgtttacc ccaaagtgga tggctccggt gatgcacaga
    53581 tggtcagcaa tatgtttgtg gcaaaaaccg gctctgtcgg tgcacaggcg gattacagcg
    53641 gcccggcctg gtatctgctt gactgctccc gcgcggtaaa accgctgatt tatcaggatc
    53701 gccgcaaagc cgaactggtt gcccagacaa aagtcgatga aggccgtgcg tttactgata
    53761 acgagtttgt gttcggtgct tccgcccgtc gcaatgtggg ctacggcttc tggcagatgg
    53821 cctacatgat gcagtcaccg ctgacgctgg atgcgctgtg gcacggctgg tcagccatgc
    53881 gtgactttaa ggctgacggt ggccgcaaac tcggcatcaa acccacccat attgttgtcc
    53941 ccacctcgct ggaaaaacag gcggtgcagc tgctggagcg cgagctgttc gcagacggta
    54001 acgccacagt ctctaacgag atgaagggca agctggagct ggttgtcgcg gattatctgt
    54061 aaacggtgag ccgggcttac ggcccggccc ctgcggaggg caaatatgaa tgaacatcac
    54121 actgtggcag caacagatga ttcaggcctt caggtcagtg gtgacagtcc tgtcacggta
    54181 ctggcagaag tgcgttgcag tcgtacgcat tttcgtcgtg cggggttcct gttcatgcgg
    54241 ggacgccagc aggttgacgt caccccggag cagcttgccc gactggaagc ggagccatgc
    54301 ctcactgtac gaatactaca gacgcctggt gatgatgcgg gagccgtggc gggtgtggtt
    54361 catgcagaga ccggtgcaga tttagccgaa gccgaaaccg tacaggatgc tccccggaaa
    54421 aagaccggca ataacgccag acaggcgaaa aaggcgcgcg catgaattac gccaccgaaa
    54481 ccgatatgcg ggcacgttac cgcgaggatt tgctcaggcc attactggcc gtgccccgtt
    54541 cggatgaacc ggacacgcgc aagctgaacc gggcactgac ggacgcgtct gcccttatcg
    54601 acagctatct gtcttcccgt tacacgctgc cgctggcggt ggttccggcg gtccttgttc
    54661 agcattgctg cacgattgcc ttttattacc tgtgcgatca gcgggcatcc gatcaggcgc
    54721 gtgaccgtta ccgtgaggcg ctggcctggc tgaaagatgt catgaacggt aacgtgccgg
    54781 tcggcgtgga tacagacggt gcggcccctg aatccgggga tttaccgcag gtgcagtctg
    54841 atgcggcggt gttcgggcgc aaccagaagg gctttatatg attacggaaa ccgagcaggc
    54901 gtacatcgcc cgtatccgtg agtatttcgg gaatgaactg gtgtctgttg acacacatcc
    54961 cggcgactgg agcgacagcg tactgcgcac catgctgatt aacgccccgg ctatctatgt
    55021 tgcctggctg ggtgccggtg aagggcgtac ccgtgggcgt ctggtcagtc actgggtgtt
    55081 ctacgtcatc ggcgacatgc tcaatggccg tgaggtcagc cgtcccgggc tttatcagat
    55141 tgtggcccgc ctgattaccg tgctggacgg cttcagagca gaaaaaacct caccgcttta
    55201 ctttgaaaag gcggtcaacg gttacaccga aacgcaggca ggcagcggtg ccgtgatgta
    55261 cgccctgtat ttctcatgcg aggaaatgat cgacccgctg acggatatca gctcgctgga
    55321 tgatttcctg cgccattacg aaacctttac cgaaccggaa ggcacgccgg aatttaaggc
    55381 acacatccgt ctgcctggtg caactgccgc cggtgaaaac gccggactgt cagaggaata
    55441 acagatgaca accattttta ttaaacctgc gccgggatgc ctgatccgcg atcccgacac
    55501 gatgactccg ctctctcagg acggggaaga gaaacccttc actccgttct ggtgccgccg
    55561 tcttaatgat ggcgacgtca ttgtggtgga taaaacggcg gagcctgccc cggctgccac
    55621 aactgctaat accggcgcgg cggcaaaacc agcgggagcg gtagcagaaa aaactgcggc
    55681 ggtggctgaa aaaaccgcag catcagataa ggggacgtcc tgatgattaa ctttgattat
    55741 atcggcgata acaatcgcat tccgctggta cagattgaaa tcaataattc aatggccgtg
    55801 accggcactc caccacagcg tcaggcggta ctgttgtttg gtcaggctgc aatgaaagac
    55861 actaccattc aggggcgcgg tcagcttgat gtaccagtac gtattacccg agcttcacaa
    55921 gcaagagaac tctgggggcg aggttccatg attgcactga tggtagagga atttatcgcc
    55981 attaaccctg acacagagtt atatgccatt gcacaggggg ctggaacagg tcagtccagg
    56041 gcatgcgtga tgaatatcat tggaacagca ttggcagacg gggtgttaag tgtttacatc
    56101 ggcggtcgcc gttacctcct gcccgtcagt aaggggaaaa aaggtaaaga gcttgtggaa
    56161 gagctggcta aggtcattaa cgctgacact gatgcgccat tcactgctat cgcagctgaa
    56221 gtcagtggtg ataatgctga aggacttaaa ggcagcatgg gggttaacgc ccgctttatc
    56281 ggtgaatgct cagctcatga tttacgggtg aactattatg acggtgaaac tacaccggcc
    56341 ggtcttacgc tggagctcgt cgcgccaaca cagaaggcaa caaaccccga tatcacccgt
    56401 agcgtctcag ggatggggaa tcgtcagtac aactacatcg tcatgcccta taaggatgcg
    56461 gcaaatctca aaattctggc ggatgaactg ctgaaacgct ggggaccggt aaaaatgtcg
    56521 gacggtatgg tctggatggc gcataccggc accttcggta cggcaaccag ttttggtgaa
    56581 tcccgtaatg acttcctgtt tacctgcact tctattccta aagcaccaga gccggattat
    56641 gtctgggctg cctccatttg tgcggtctgt gcgccctccc tgtcaacgga tccggcccga
    56701 ccattacaga cactggcgct gcctgcccgt atggcacctg aagcaccgga tcgtctgacg
    56761 cgggaagagc gaaacagcct gctttatacc ggcatgtcca cggtgaccgt cgcggccgcg
    56821 gatgtggtgc agattgaacg tcaggtgacg atgtaccgtc agaatgcata cggcgaaatc
    56881 gatcccagct atctggatgt ggaaaccatc tacaccctgt catatctgcg ttattccctg
    56941 cgcaccttta tcacgcagcg ttttccgagc cataaactgg ctgatgacgg gacgcccgtt
    57001 cgtgccggtc agaatatcgt gacaccggag atcatgaaac ttcagttaat ttcgctgggg
    57061 gaagaatggg tggatctggg actggtcgaa aatctggaca cctttaaaaa gaatctgctc
    57121 gtggagcgca acacaaaaga ccgtaaccgt ctggatgtga tgtgtacacc ggatctggtt
    57181 aaccagttcc gcttcatggc cgcgcagatc cgtttcattt tgtgaggtaa ggcatgagcg
    57241 gaaaacagta tcagggcacg gccaccatcc gtgtgaacgg gcaggagtac gacacccttg
    57301 agggggccac gttctccccg tccggctttg agcgtgaagt ggtgaaaggc gcgaaagttt
    57361 acggctatcg cgagaaaccc cgtgaggcga cgctggactg taaattcccg gcgggcggag
    57421 aaggttcacc ggctgccgat gaaatcaaca cctggactgc ggtcacgatt gagttcgtgg
    57481 ccgataccgg tgaagtccac atgatgacga aggcctggag cagtgaaccg gcctcgcttg
    57541 acggtggtgg tgatatctcc gcgaagttcg ccagcgccac cagtacccgt gtccagtaat
    57601 caggaaaaat gtaatgagca cacgtaagaa aaaaacggcg gtttctgacg aggccgtgat
    57661 ggaggctatc cgggatgcac ttgaagacgg cgatccccgc accgccgggc tggctgaaca
    57721 actggcgaac ggctatgtgg atctgctcga cggtctgccg ttcggggaat cccgtgaata
    57781 ccgcgtcacc ttccgcgaac tgacggcaaa agacagcatt gatgcggaga gcgaggccga
    57841 gcgtcttatg gaaacccgaa acggtccggt tcttgtggcc tcaccggcac tgcgcggcat
    57901 tgcgctgctg cgccgtcaga ttgccgctgt cggccagatt gaggggccat tgtctccgcg
    57961 tcagatagga cagctcagcg agcgcgatct ctcccgactg atggcggcag taagcctgct
    58021 tgattccgcg atggccggaa agctggtcgc tgaacggggg cgaccagatg cagtgccggg
    58081 atcagattga agaagccgca attgtgctgg ggatggtgac aaaaagcggc ccggagtggg
    58141 cgcttaacct ccccttatcg cagctttacc ggcactgccg acagaccgaa aaaattctca
    58201 gaacgaagca gtaacccatg gcaaaaaacc tgaaagcctc cctgattgtc gatttactcg
    58261 gcaatatttc tgccaaatcc cgccagtggt cacaggagct gggggcattc tcccgttccg
    58321 gcaggacggg gctgggtggt cttggaaatg cagcacgtcg tgccggtcag gagacagaac
    58381 ttgtcggctc ccgtatgcag cgaacgcttg ccggtgttcg tggcagtatc cgcacggtga
    58441 cgtcagattt tgaccgcctt cagggcagca ttaccggcac catcgggcgg ataagcaacc
    58501 tttacgggat gctggcggga ggggctgcgg tctacgggtt taataaagcc tttatccgcc
    58561 cggccgcaga gatggagaac tatattctgc gtctcaacgc cattaatcat ggcgatacgg
    58621 caaaaacgga agcagtgaaa gcctgggcgg tacagaacgc caaagacacc acctgggggc
    58681 tggcgggggt tatgcaggaa tacgcctcca gtcgcgggtt cggtatgagt gacagggaag
    58741 cccgtcgctt tattaccatg cttcaggatc agggcggcta tcacggctgg tcactgtcag
    58801 atgcacaggg tgcgtccctg caactcaaac agatgtttgc ccgccagagt atccaggcag
    58861 cggacgccaa tattctgacc ggatacggga ttaacgttta ccagttgctg gccgataagc
    58921 tgggtgtaaa ccagaagatc atccgtgaga aaggcgagaa aggaaaactg ggaccggaca
    58981 gcattcgcct gttatttcag gtgatggccg aacaggcaaa aggtgcccag aaaaacgcca
    59041 tgaattcctg gacgggaatg acgtccatga tgggcgatgt ctgggacgga tttgcccgcg
    59101 aggtgatggc aaagggaccg tttgacagcc tgaaaaagag cctgaaaggc tttctggatt
    59161 atgctgatgc ggcacagaaa agcggcttac aggataagct ggccacacag acggcctcag
    59221 ccctgaatca gggatttgag tatgccagag acgctgcgac cggcttttac cgggtcattc
    59281 agaaagtcag ggaaacgctt caggcactgc gcgacgccgg ttatggcgat gcgctggatc
    59341 gcattggtca gggtgcgcag accgccgcaa agtacctgat gtatatgtat ctcgcctccc
    59401 gtgcgctgaa ggtgctcagg tttgcgggga cgggggcatt acgtctcggc gcaaccccct
    59461 tacgctatgg catggcgatg acctccgtgc tgacgtcgcc cttccgcaaa ccgcagacca
    59521 ctgtaccggg aacgcagccc ggacgcgctg gccggttcct gaatttcctt accggggtaa
    59581 atccggctgc cgttcagccc gtgctggtca ccaactggcc tgcgggtggc ctggcctcag
    59641 gtggcgggga tgtggtggtc agcggtgacg gcaaaaccgt gcgcggccgt aaaaaacgcg
    59701 gccccggacg tggacggggt gtcacgaccg tcgtgactgc cggtgagcaa ctggcggaga
    59761 gtgccggtaa acagggcttc ttcggtcgta tgatgagccg tgccggtggt ctgctaacgg
    59821 cggccggaaa ccgcatgggg ctggggcgtt ttgccgggct gttccgtggt gccggtcggc
    59881 tgggtggtgg tgccctgtgg gccggtgcca tggcggctcc tgtactgctg gacagcagcg
    59941 ccagtgcagc cgataaagcc ggtgccgtgg gttcccttgc cggaagtatc gccggtggcg
    60001 cgctgggggc ggctgccggt ccggtcgggg ttgccattgg ttccacggtg ggcagctatc
    60061 tcggtgatta tctggggggc tggctgacgc aggcctggca gaaactgcgt ggcggcagtg
    60121 acgaaaacgg cggacaggcc accgcgaaaa ccgccgcacg ggtggagctt gttgcccctg
    60181 aaggctggcg ggcgcgcagt attgatgtgg atgataccgc acagcatggc ctggatgtga
    60241 acgtctggaa cggagggaac tatggcctct actgacacgc cggggcgtgg ctcctttcgt
    60301 ggcgtaccgt tcctggttta tcaggagcag cgggaacggg gcggacgtaa cattgtgcgc
    60361 cgtgaatacc cactgcgtga aagcggcggt gcggatgatc tggggccaaa actcccggag
    60421 ttcacattca cggttctggt gaccggagac gatcttcaga cacagcgaag ccgcctgcgt
    60481 gatgcgctgc gtgcacccgg tgccggtgaa ctgatgcacc cggattacgg cacgttaaac
    60541 gtgctgataa acagctttga aagccgttat aacgccagcg agcagggaac ggttgagttc
    60601 acgatcaacg tgatccccgc cagtgacgat accgccccta cggtggcaga agatacggca
    60661 gctgcgctgg agcagaaaag cggctcagca atgaatcagc tgtttaacac gctttcagac
    60721 ggctggacgg tgatttcaga cggtctgcat gatgtacagg ccatgaccga caccatcagc
    60781 gataagattg atgcactgga gaatgccgtt tcggggatgg ggattgtgca ggatatcagt
    60841 gcctttacgg ccagttttac tgccctgaaa ggcaatgccg ccgccctgat taatgcgccc
    60901 cgtcgcatgg ctgaatcgct ggccggaatg tttgccgtgc tgaccgggct gcccggcgat
    60961 ccttccctgt ctctgaccgg aaaagccgga acgccgtccg gcagcctgac cacaaaccgt
    61021 gacagcatgg cagagcaggc catgccgcag ctgtaccgca cactttcctc cctgcgttac
    61081 accctcagcg aacaggatga tccacagcgc ctgattggcc tgacgcctgc ggcacagaaa
    61141 aatatccgtc tgctgcggag cgtgatgcag agtgcaaccc tggtgtcgca ggcacagacg
    61201 ctcggaaaac tgctggatca ggtcatccgg cagaatacga cgcacgttca ggatactgac
    61261 cgttcgggcg gactggcctg gctggaaagt tctgctgatg tgcagcgtat caaccgggat
    61321 ttgagcgatg ccatggaaca acaggtgctg gatctgtccg cacagggcta taccgggact
    61381 gcccttgcat tacgtgatgc agggcttgcg ctgacagaag atttaaccac acggagcgtg
    61441 catcttcccg gtgcttttca ggtcatggtt cgcaccaccg aaccggcact ggtcacgctg
    61501 taccgtgcca ccggcaacag cagtcgctgg cagtattttg tccgcagaaa caatatcccc
    61561 gatccggttt ttgttccggg tggccgcagc gtggaggtga tcagtgagca gcaggattga
    61621 actgtatatc ggtggcagta ttttttccgg ctggctgacg gtcagcgtcc gtcgctcgct
    61681 ggagcatctg gccgggtcgt ttgaactggg gctgatgctg cccggtgagc gcatcccctc
    61741 agccctgcga accggtcagt ccctgacgct cagaattaac gggcagacgg tcatcagtgg
    61801 ctggctggat caggtcagtc agcggatcag tgcaacccgt catcagatca gcatcagcgg
    61861 tcgggataaa accggcgacc tggtggactg tgccgccatc catcccggca gccagtggcg
    61921 caaccggacg ctggcacaga ttgccgcaga tttatgtgcg cccttcggga tagccgtgcg
    61981 ctggcaggtt aacgacgaca cggctgcacg ccccttcagc tcttttacgc tggaaaactc
    62041 agaaaccgtg gcggatgcgc tgacccgtgc tgcccggcat cgcggggtgc tggtgaccag
    62101 taatgctgac ggcgatctgg tgttcaccca ggcaggaagc cagcagacgg acagactggt
    62161 gctgggagat aacctgcttg atgccgatta caacacggac tggcgaggac gatacagcga
    62221 ataccgtgtc cggggacacg ggcgcggtgg tggcaaacgg ggagacagcg aatccgccgc
    62281 ccgtctggca gcacctgtgg gcgtcatcag tgatgagcag atcggccgct accggtcgaa
    62341 aatcatcctc gccgatcaac agacagacac caccggtgca cggcagcgtg ccctgcgtga
    62401 aatgcgccgt gcgattgccc gttcagaacg gttttctgcc accgtgcgtg gctggttccg
    62461 ggatgatggc cggttatggg atgtcaatct gctgaccggt gtttcagccc tgcgtttcgg
    62521 tatagaacag actgaactgc tggtctgtca ggtggagttt ttacttgatg aacataacgg
    62581 ggaagtcacc cggctggtac tggcaccgcg tgacggcttt atcgttccgg cagagccgga
    62641 cagtaagggc cggggtggtt ccggtgacga tgttgatgcc tttattcgcc agcagatgaa
    62701 aaaacagggg atcagctttg atgaatgatg aagtgttcag ccgcctgatt gccccggtaa
    62761 cgcgcggtat tcgcctgttg tttggccggg gcgttctgac cggcacacat gacgaactga
    62821 aaatgcagaa tgtgcagctc accggcatgg acggcgaaac ctttgatgat gtggagcgtc
    62881 cccagcagta cgggcagatc agcgttcccc tgccgggtgc ggaaaccttt tttgcctgtc
    62941 tgggcggaca gcgggatcag actgtggtgc ttgtggtgga agaccggcgc agccgcccga
    63001 ccggactcac agccggagat acgggggtgt atcaccatga ggggcaccgg atacggttaa
    63061 caaaggatgg tcgcattatt gtgacatgca aaatgctgga gatttacgcc gatgagggga
    63121 tgcgggtgga tacgccggag gccaccttta cgggcaatgt gacggtggat aaaaacctgc
    63181 atgttaaggg taatttcgtg cttgatggta caggtaaatc agacgggctg ttcacgatgt
    63241 ctgatgccgt tattgccggg atcacctatt ccggccatgt gcatcaggat aacggcaaag
    63301 gcagtaagac aggagcgccg gagaatggct gatattgcaa ttgtatggga tcaggggtgc
    63361 ggttcgctgc aactgaacgg tgccgacttg ctgacggatg acagtctgct gacggccgtt
    63421 ctgatttcgc tgtttactga ccgccgggcg ctggcatcgg atgaaatccc tgatggtacg
    63481 cgtgaccgcc ggggatggtg gggagacagt tttcgcccgc gtcccattgg ctcccgtctg
    63541 tggcttctcc gccgggaaaa aacactggcc tccgtgataa gccgtgcccg tgcctatgcg
    63601 gatgaggcgc tgggctggct cagtcaggat ggcgtggcgt catccgtggt ctgtcatgca
    63661 gaacgtgtgg ggcacgcgcg gctggcgctt tcggtgcgca taaccctgcc tgatggttca
    63721 gtgaggccca tgatttttta tgctgatctt aaggggcagt aatgccttat cagcctttac
    63781 cgctggcaca gttaatcacg cagacacagc aggatatcag ccagcgcctg cccggctcgc
    63841 agccgggtgt gaatgaaacc accctgaatg ccattgctta cgctcaggca gggttatctg
    63901 ctcaggagca tgagcatctg gcctggattg cgcgtcagat catcccgacc gaagccgatg
    63961 aagccgaact gctgaaacac tgtgcattct ggggcgtcat gcgtaagcct gcctctcggg
    64021 gggacgggcc ggttcaactg atgctgacca cggatgcggg gatcaaggaa ggtgtgcttc
    64081 tccagcgaag tgatggcgtg gtttaccgta tcaccacctc actgaccggt aaggccggta
    64141 cgatgaatgt cagtgtggaa gccgaaagtg ccggtcgtgc ggggaatgcc ccggcaggga
    64201 caaaactgac atttatcaca ccgcaggcgg ggatcaacca gacggccacg gtgaccggaa
    64261 cggggatcac cggtggtgcg gatgtggaaa ccgtgccgga gctgctttcc cgtctggttt
    64321 tccgggtgca gaatccgccg tccggcggca cgcagtatga ttttgaacgc tgggcgcgtg
    64381 aagtgccggg cgtgacgcgg gcatggtgcc gcccggaatg gccgcaggcg ggcagcgtgg
    64441 gggtaacgtt tgttcaggat aataacccgg atattttccc cggtgacggt gatgttcagc
    64501 gggtggcgga ttatatccgc agtcatgatg atccggcgac cggccagcct gtcggacagc
    64561 ctctggggcc gacggtgacc gtgtttaaac tgaccaataa gccggtgccc ttcagcatca
    64621 ggatcatccc gaaaacaccg gagaatcagg cggccgtaaa acaggcgctg accgacctgt
    64681 tgtataacga atcccggccc ggcggtctgg ttctgccgtc gtctttctgg cgggcggtgg
    64741 caggggtgaa aaatctggag gattttgaag tgcgcagtcc gctgacgtca gttcaggcgg
    64801 gagacagtga gctgctgacg gtaggagaaa tcacatggct gtaaccctga ccccgcatca
    64861 gcgcgccctg ttgcagttac tgcctgacgg gctggcatgg gacaagcgtc cgtcatccgt
    64921 actggcatca ttatgtctgg gcctcagtca ttcgacagcg cgtgtatcct ggacgggtaa
    64981 acagctgctt gccgagcgtt tccctgacac gtcgcgcctg ctgctggaag actgggagcg
    65041 ttatctcggg cttcccgagt gtgatatggc cggagccacc ctcacggagc gacagcgtta
    65101 tgccggaaat aaataccgga tgaagccctc gctgaaccgt gaattttata tccggtttgc
    65161 ggcggagttt ggttatcaga ttgacattca gccctcaccg gattcgcagt gggtcagtat
    65221 tgtcacgatt aacagtgaaa ccggctaccg gaatatgaat gtgctggatg atattctcac
    65281 gccgctgcgt atttatgaag gcggtgcgct ggaatgtatt ctgaaccgtt ataagcctgc
    65341 atggcagacg tttatttacg tttatgcaaa cagccatgaa gaggagacta tttaatgttt
    65401 catgttgata ataattccgg cgtggcgaat atgcctgcgc tggcaccggc gcagagtaat
    65461 accaccacct ggtttaccga aggtgacgga caaaaaggta tcagctggat tggtcaggac
    65521 tggctgaata ttctccaggc cgaactgctg aatattctgg ctgaagccag tattcagccg
    65581 gataaggcgc agttaaacca gcttacgctg tccattaaag ccattatcgc tgcgaatgcc
    65641 ttttcccgga aaaataacct gaaagaaatt gctgatgccg gtgcggaggc ccagcgtctg
    65701 gcccgtggtt atcttggtct ggggacgctt gccacaaaaa acagtcttgg tcccgctgac
    65761 gttaatgccc tggcgaagga tcagaatctc gccgacctgg agaataaggg aaccgcccgt
    65821 aataatctgg atgtttacag caaaagcgaa ggtgataacc gttacctgcg cagggagcag
    65881 aacggcgcag acattccgga taaaggggct tttatcgata acgtcggttt acgggaaacg
    65941 gtaaacaggg cggcgaatgc cctgccatcg gacggcaccg ccgttgccgc gaacaggctg
    66001 gcaaatgccc acacaattaa tggtgttccc tttgacggga cgcaggatat cactatctca
    66061 tcaggaacag taaccgccat aagactgggg tcggtgaccg cacatatgcc cggaacatgg
    66121 gaaagctggg atctgaatct ggggggcggc aatgttctta ccggaattaa agtacaggat
    66181 gtcggtaaga atacggcaga taacgtcggc ggcgtgtatt accggccgtt gcagtatctg
    66241 ttaaatggag catgggtgac ggctgcaagt atataatgat attcagaagg ccttccggcc
    66301 ttctttatta ctgtggctgc tcaggccatt taatggcgtt aaattcagct tctgttttaa
    66361 tggcgggtag ttccattttc ttcacctgac tgatgtatgc catccattgg gtaagtgctg
    66421 ctctgtcttc atcgctgatg gtgcccagtt gtagctccgt tctccagtca ttaatttttt
    66481 catatgcctg attaagatat gactggcgca ttgcttccag ttttcttgcg tagttaaccg
    66541 gaatacggga gattatgcca ttatcaaact tccagtttcc tgaaatatcc gtgccttcag
    66601 ggagttcatc gacctcaacc acagaaaacc ccgcaggata cagcgccgag gcgtcctgtg
    66661 atattgagca gataacgccg ctgtcaggtg ttatgcaaag tttatatttt ttggtgaata
    66721 atggcaggga ttcgtagaaa tccttaccat cttcactctg gaaatactga acattattac
    66781 catatggcat attttcagga taataacggg ttatattttt tagttccata
//
LOCUS       DAZFFE010000011        65136 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFFE010000011 DAZFFE010000000
VERSION     DAZFFE010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.011"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..474)
                     /gene="folK"
                     /locus_tag="ACHJ69_000891"
     CDS             complement(<1..474)
                     /gene="folK"
                     /locus_tag="ACHJ69_000891"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO5013939.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALKTTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGLSLVEALQAKGFNELDK"
     gene            complement(471..1868)
                     /gene="pcnB"
                     /locus_tag="ACHJ69_000892"
     CDS             complement(471..1868)
                     /gene="pcnB"
                     /locus_tag="ACHJ69_000892"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO5013940.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1928..2824)
                     /gene="gluQRS"
                     /locus_tag="ACHJ69_000893"
     CDS             complement(1928..2824)
                     /gene="gluQRS"
                     /locus_tag="ACHJ69_000893"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO5013941.1"
                     /translation="MTDTHYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLYWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRDLHHGPDNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALHFLGQQVETHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            complement(2891..3346)
                     /gene="dksA"
                     /locus_tag="ACHJ69_000894"
     CDS             complement(2891..3346)
                     /gene="dksA"
                     /locus_tag="ACHJ69_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO5013942.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3524..4228)
                     /gene="sfsA"
                     /locus_tag="ACHJ69_000895"
     CDS             complement(3524..4228)
                     /gene="sfsA"
                     /locus_tag="ACHJ69_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO5013943.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAIICVNTLWANRLTKEAILNESISELA
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(4243..4773)
                     /gene="thpR"
                     /locus_tag="ACHJ69_000896"
     CDS             complement(4243..4773)
                     /gene="thpR"
                     /locus_tag="ACHJ69_000896"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO5013944.1"
                     /translation="MSEPQRLFFAIDLPAEICEQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4847..7276
                     /gene="hrpB"
                     /locus_tag="ACHJ69_000897"
     CDS             4847..7276
                     /gene="hrpB"
                     /locus_tag="ACHJ69_000897"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO5013945.1"
                     /translation="MSSLPVAAVLPELLAALDGASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQTLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLTSRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWMDQPPTVNLLAAKRL
                     LRMLGALDGERLSAQGQKMATLGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAYADRIARRRGQEG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLMVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWVMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7370..9898
                     /gene="mrcB"
                     /locus_tag="ACHJ69_000898"
     CDS             7370..9898
                     /gene="mrcB"
                     /locus_tag="ACHJ69_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO5013946.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGRKPRGKRGWLWLLLKLAIVFAVMIAIYGVYLDQKIRSRIDGKVWQLPA
                     AVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDFP
                     DSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRSG
                     FPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFL
                     SSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYFG
                     RPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDML
                     SARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEK
                     AAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSIGS
                     LAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLVDA
                     LTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLNPVPAMLLGALNLTPIEVAQAFQ
                     TIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTGR
                     QLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAMSI
                     YQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSEMQ
                     QQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10118..12361
                     /gene="fhuA"
                     /locus_tag="ACHJ69_000899"
                     /pseudo
     CDS             10118..12361
                     /gene="fhuA"
                     /locus_tag="ACHJ69_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
     gene            12412..13209
                     /gene="fhuC"
                     /locus_tag="ACHJ69_000900"
     CDS             12412..13209
                     /gene="fhuC"
                     /locus_tag="ACHJ69_000900"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO5013947.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAAEREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13209..14099
                     /gene="fhuD"
                     /locus_tag="ACHJ69_000901"
     CDS             13209..14099
                     /gene="fhuD"
                     /locus_tag="ACHJ69_000901"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO5013948.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAVIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAVYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            14096..16078
                     /gene="fhuB"
                     /locus_tag="ACHJ69_000902"
     CDS             14096..16078
                     /gene="fhuB"
                     /locus_tag="ACHJ69_000902"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO5013949.1"
                     /translation="MSKRIALFPVLLLALLVVAAAALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLVKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16113..17393)
                     /gene="hemL"
                     /locus_tag="ACHJ69_000903"
     CDS             complement(16113..17393)
                     /gene="hemL"
                     /locus_tag="ACHJ69_000903"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO5013950.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LREAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAAHRVFAKL"
     gene            17559..17606
                     /gene="yadX"
                     /locus_tag="ACHJ69_000904"
     CDS             17559..17606
                     /gene="yadX"
                     /locus_tag="ACHJ69_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO5013951.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17618..19039
                     /gene="clcA"
                     /locus_tag="ACHJ69_000905"
     CDS             17618..19039
                     /gene="clcA"
                     /locus_tag="ACHJ69_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO5013952.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVVMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASARENT"
     gene            19063..19128
                     /gene="yadW"
                     /locus_tag="ACHJ69_000906"
     CDS             19063..19128
                     /gene="yadW"
                     /locus_tag="ACHJ69_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO5013953.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19121..19465
                     /gene="erpA"
                     /locus_tag="ACHJ69_000907"
     CDS             19121..19465
                     /gene="erpA"
                     /locus_tag="ACHJ69_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO5013954.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19512..20135)
                     /gene="yadS"
                     /locus_tag="ACHJ69_000908"
     CDS             complement(19512..20135)
                     /gene="yadS"
                     /locus_tag="ACHJ69_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO5013955.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20173..20973)
                     /gene="btuF"
                     /locus_tag="ACHJ69_000909"
     CDS             complement(20173..20973)
                     /gene="btuF"
                     /locus_tag="ACHJ69_000909"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO5013956.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANVLRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20966..21664)
                     /gene="mtnN"
                     /locus_tag="ACHJ69_000910"
     CDS             complement(20966..21664)
                     /gene="mtnN"
                     /locus_tag="ACHJ69_000910"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO5013957.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21748..23265
                     /gene="dgt"
                     /locus_tag="ACHJ69_000911"
     CDS             21748..23265
                     /gene="dgt"
                     /locus_tag="ACHJ69_000911"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO5013958.1"
                     /translation="MTQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDVESQPLTDDRCSVAAL
                     RLRDGEEPLNALRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RVETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLNLPLSDFTELVEKERVKRFPIETRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23395..24819
                     /gene="degP"
                     /locus_tag="ACHJ69_000912"
     CDS             23395..24819
                     /gene="degP"
                     /locus_tag="ACHJ69_000912"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO5013959.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGLGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSDLAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSST
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            24974..26131
                     /gene="cdaR"
                     /locus_tag="ACHJ69_000913"
     CDS             24974..26131
                     /gene="cdaR"
                     /locus_tag="ACHJ69_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO5013960.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26184..26570)
                     /gene="yaeH"
                     /locus_tag="ACHJ69_000914"
     CDS             complement(26184..26570)
                     /gene="yaeH"
                     /locus_tag="ACHJ69_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO5013961.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26889..27713)
                     /gene="dapD"
                     /locus_tag="ACHJ69_000915"
     CDS             complement(26889..27713)
                     /gene="dapD"
                     /locus_tag="ACHJ69_000915"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO5013962.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFANYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27744..30416)
                     /gene="glnD"
                     /locus_tag="ACHJ69_000916"
     CDS             complement(27744..30416)
                     /gene="glnD"
                     /locus_tag="ACHJ69_000916"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO5013963.1"
                     /translation="MNTLPEQYANTALPTLHGQPQNPCAWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASKETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLHALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30478..31272)
                     /gene="map"
                     /locus_tag="ACHJ69_000917"
     CDS             complement(30478..31272)
                     /gene="map"
                     /locus_tag="ACHJ69_000917"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO5013964.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31640..32365
                     /gene="rpsB"
                     /locus_tag="ACHJ69_000918"
     CDS             31640..32365
                     /gene="rpsB"
                     /locus_tag="ACHJ69_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO5013965.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32500..33351
                     /gene="tsf"
                     /locus_tag="ACHJ69_000919"
     CDS             32500..33351
                     /gene="tsf"
                     /locus_tag="ACHJ69_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO5013966.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33498..34223
                     /gene="pyrH"
                     /locus_tag="ACHJ69_000920"
     CDS             33498..34223
                     /gene="pyrH"
                     /locus_tag="ACHJ69_000920"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO5013967.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITK"
     gene            34373..34930
                     /gene="frr"
                     /locus_tag="ACHJ69_000921"
     CDS             34373..34930
                     /gene="frr"
                     /locus_tag="ACHJ69_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO5013968.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35022..36218
                     /gene="ispC"
                     /locus_tag="ACHJ69_000922"
     CDS             35022..36218
                     /gene="ispC"
                     /locus_tag="ACHJ69_000922"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO5013969.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36407..37165
                     /gene="ispU"
                     /locus_tag="ACHJ69_000923"
                     /gene_synonym="uppS"
     CDS             36407..37165
                     /gene="ispU"
                     /locus_tag="ACHJ69_000923"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO5013970.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37178..38035
                     /gene="cdsA"
                     /locus_tag="ACHJ69_000924"
     CDS             37178..38035
                     /gene="cdsA"
                     /locus_tag="ACHJ69_000924"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO5013971.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRIIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWVNLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38047..39399
                     /gene="rseP"
                     /locus_tag="ACHJ69_000925"
     CDS             38047..39399
                     /gene="rseP"
                     /locus_tag="ACHJ69_000925"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO5013972.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGKGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39429..41861
                     /gene="bamA"
                     /locus_tag="ACHJ69_000926"
     CDS             39429..41861
                     /gene="bamA"
                     /locus_tag="ACHJ69_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO5013973.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            41983..42468
                     /gene="skp"
                     /locus_tag="ACHJ69_000927"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             41983..42468
                     /gene="skp"
                     /locus_tag="ACHJ69_000927"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO5013974.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42472..43497
                     /gene="lpxD"
                     /locus_tag="ACHJ69_000928"
     CDS             42472..43497
                     /gene="lpxD"
                     /locus_tag="ACHJ69_000928"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO5013975.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43602..44057
                     /gene="fabZ"
                     /locus_tag="ACHJ69_000929"
     CDS             43602..44057
                     /gene="fabZ"
                     /locus_tag="ACHJ69_000929"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO5013976.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44061..44849
                     /gene="lpxA"
                     /locus_tag="ACHJ69_000930"
     CDS             44061..44849
                     /gene="lpxA"
                     /locus_tag="ACHJ69_000930"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO5013977.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            44849..45997
                     /gene="lpxB"
                     /locus_tag="ACHJ69_000931"
     CDS             44849..45997
                     /gene="lpxB"
                     /locus_tag="ACHJ69_000931"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO5013978.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            45994..46590
                     /gene="rnhB"
                     /locus_tag="ACHJ69_000932"
     CDS             45994..46590
                     /gene="rnhB"
                     /locus_tag="ACHJ69_000932"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO5013979.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            46627..50109
                     /gene="dnaE"
                     /locus_tag="ACHJ69_000933"
     CDS             46627..50109
                     /gene="dnaE"
                     /locus_tag="ACHJ69_000933"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO5013980.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGTAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAESRGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLDTELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50122..51081
                     /gene="accA"
                     /locus_tag="ACHJ69_000934"
     CDS             50122..51081
                     /gene="accA"
                     /locus_tag="ACHJ69_000934"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO5013981.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51179..53320
                     /gene="ldcC"
                     /locus_tag="ACHJ69_000935"
     CDS             51179..53320
                     /gene="ldcC"
                     /locus_tag="ACHJ69_000935"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO5013982.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RGEFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFSDADANHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTEESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKHDEDGVYRVRVLKMAG"
     gene            53377..53766
                     /gene="yaeR"
                     /locus_tag="ACHJ69_000936"
     CDS             53377..53766
                     /gene="yaeR"
                     /locus_tag="ACHJ69_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO5013983.1"
                     /translation="MLGLKQVHHIAIIATDYAMSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            53831..55144
                     /gene="tilS"
                     /locus_tag="ACHJ69_000937"
     CDS             53831..55144
                     /gene="tilS"
                     /locus_tag="ACHJ69_000937"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO5013984.1"
                     /translation="MTLTLNRQLLSSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVKHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     HTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTQLIRPLLARTR
                     GELVQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTPQGTLQIALMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVAGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAVGENGVSFVWQKTLSQVKAG"
     gene            complement(55178..55438)
                     /gene="rof"
                     /locus_tag="ACHJ69_000938"
     CDS             complement(55178..55438)
                     /gene="rof"
                     /locus_tag="ACHJ69_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO5013985.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGATRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55425..55625)
                     /gene="yaeP"
                     /locus_tag="ACHJ69_000939"
     CDS             complement(55425..55625)
                     /gene="yaeP"
                     /locus_tag="ACHJ69_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO5013986.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            55791..56336
                     /gene="yaeQ"
                     /locus_tag="ACHJ69_000940"
     CDS             55791..56336
                     /gene="yaeQ"
                     /locus_tag="ACHJ69_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO5013987.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTVWQQPS"
     gene            56333..56755
                     /gene="arfB"
                     /locus_tag="ACHJ69_000941"
     CDS             56333..56755
                     /gene="arfB"
                     /locus_tag="ACHJ69_000941"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO5013988.1"
                     /translation="MIVISRHVAISDGEFEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRNQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            56769..57479
                     /gene="nlpE"
                     /locus_tag="ACHJ69_000942"
                     /gene_synonym="cutF"
     CDS             56769..57479
                     /gene="nlpE"
                     /locus_tag="ACHJ69_000942"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591565.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO5013989.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARSNSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGL"
     gene            complement(57635..58459)
                     /gene="yaeF"
                     /locus_tag="ACHJ69_000943"
     CDS             complement(57635..58459)
                     /gene="yaeF"
                     /locus_tag="ACHJ69_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFO5013990.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATVVNAEAKTWAV
                     KFQHPSSFTEQSIKEITEPDLKPGDLLFSSSLGVTSFDIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAIKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKPQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDISAFKPETQLQYVGHMKPGIYIKAGRFVGLTQ"
     gene            complement(58512..60230)
                     /gene="proS"
                     /locus_tag="ACHJ69_000944"
     CDS             complement(58512..60230)
                     /gene="proS"
                     /locus_tag="ACHJ69_000944"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO5013991.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPSELWQESGRWEQYGPELLRIADRGDR
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFLVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVDYL
                     VKQIKG"
     gene            complement(60341..61048)
                     /gene="tsaA"
                     /locus_tag="ACHJ69_000945"
     CDS             complement(60341..61048)
                     /gene="tsaA"
                     /locus_tag="ACHJ69_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO5013992.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVCFTAEVEKQLLTLKKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61045..61449)
                     /gene="rcsF"
                     /locus_tag="ACHJ69_000946"
     CDS             complement(61045..61449)
                     /gene="rcsF"
                     /locus_tag="ACHJ69_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO5013993.1"
                     /translation="MRALPICLVGLMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61567..62382)
                     /gene="metQ"
                     /locus_tag="ACHJ69_000947"
     CDS             complement(61567..62382)
                     /gene="metQ"
                     /locus_tag="ACHJ69_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO5013994.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62422..63075)
                     /gene="metI"
                     /locus_tag="ACHJ69_000948"
     CDS             complement(62422..63075)
                     /gene="metI"
                     /locus_tag="ACHJ69_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO5013995.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTISAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63068..64099)
                     /gene="metN"
                     /locus_tag="ACHJ69_000949"
     CDS             complement(63068..64099)
                     /gene="metN"
                     /locus_tag="ACHJ69_000949"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO5013996.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64287..64859
                     /gene="gmhB"
                     /locus_tag="ACHJ69_000950"
     CDS             64287..64859
                     /gene="gmhB"
                     /locus_tag="ACHJ69_000950"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO5013997.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMFLSARDYLHIDMAASYMVGDKLEDMQAAAAASVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15339 a        16166 c        17962 g        15669 t
ORIGIN      
        1 cttatctaat tcattaaagc cttttgcctg tagagcttca actaatgata agccatcagg
       61 aaataccaat tccggcgcaa tttcaaacag tggccacagc ataaagccgc gatttttcat
      121 atcgtagtgc ggaacggtca gacgttcggt atttattact tcattaccaa acagcatgat
      181 gtcgagatcc agcgtgcgcg gtccccagcg ttcagcttta cggacgcgac cttgctgcaa
      241 ttcgatacgt tgtgtgtgat tgagtagctc ttcaggggca agagtggttt tcagcgcgac
      301 ggcggcattt aagtaatcgg gttgatcttg cggccccagc ggtggggtgc ggtaaaacga
      361 agaaacagca agaatgcggc tttctggaat atcgcctaat gctttcaggg cagcattgac
      421 ctgctccagc ggagaggcca gattgctgcc tatggcaata tacgccactg tcatgcggta
      481 ccctcacgac gcggtgcgcg tttgcgtgga cgacgagtac gacgacgcgg tgacggttct
      541 tcatccagtt cgttaagcat ccctttttgg tctggtggcg cggaaacctg gaactcaccc
      601 caccatttca ccagacgttg cagttcagcg ttacgctcaa cttcggctcg taaggccaac
      661 aggtcgtatg ccgcacggaa cttaggatgc tccagcagtt tccatgcgcg ttttccctga
      721 cgacgggaca tacgcaactg caactgccag atatcgcggg ttagcgtggt caaacgtttc
      781 gggatcgcca gtgaacggca ggcttcgtcc agcacgtcgt tcatcgccag tgcgaaagcg
      841 tcgtgatagg tcaggccgct ttcctgggcg atcttctgtg ccgtctccaa caatgggtac
      901 cagaacatgg cggcaaacag gaacgccggg ttcacgcgca tatcgttatg gatacgcgta
      961 tcggtattct tcagtacctg ctcaataatg cgttccatcg ggctgtcgcc attttccgtg
     1021 aagtagcggg taatggtcgg gaacagcggc tggaacagat gatattcaca cagcagctta
     1081 taggtttcgt aaccgtagcc cgcttgcaac agtttcagcg attcttcaaa cagacgtgcc
     1141 ggtgggatat cgttcagtaa ggttgccagg cgtgggatcg gctcggcggt ttccgggctg
     1201 atgcgcatgc ccaatttggc ggcaaaacga accgcgcgga gcatacgtac cggatcttca
     1261 cggtagcgcg tttccgggtt accaatcaga cggataacgc cgtctttcag atccttcatg
     1321 ccgccaacgt aatcacggac ggtaaaatct gccacgctgt aatacaggct gttgatagtg
     1381 aaatcgcggc gctgggcgtc ttcttcgatg gagccgaaaa tgttgtcgcg cagcaacata
     1441 ccgttttgcc cgcgttggga ggtcgtgcgg tcgctgacgt taccttcgtg atgtccacgg
     1501 aaggtcgcaa cttcgataat ctccgggcca aacatcacat gagccagacg gaaacggcga
     1561 cccaccaggc ggcagttacg gaacagtttg cgcacctgct ctggcgtggc gttggtggtc
     1621 acgtcaaaat ctttcggctt tttgccaagt aacaggtcgc gcacgccgcc gccaaccagc
     1681 caggcttcgt atcccgcttt attgagcctg tacattacct tcagggcatt ttcactgata
     1741 tctttgcggg aaatagcatg ctgctcacgc gggatcaccg tcacctgtgg acgggcgact
     1801 gcctgttcag cctcgctttc ctcgcggctt agcaccttgc ggcaaaaatt agcgactcgg
     1861 gtaaaaatag tacacctcgg tagtgtcaaa catcattcag gacaaaaaaa tagcggctaa
     1921 tcatagctca gcatgacgca tttgagaatg ttgaatttac aattgccgac tcaggcacgg
     1981 cggtcagcgt ccagtttttg acggctgact gaaggatttg ctcgacgctg aaatcctgcc
     2041 agtgtgtttc tacctgctga cccagaaaat gaagtgccgc gattaacacc gggcgcggat
     2101 cgcctttcgg caacgcaggc gcatgattct gcttggaaag tttagcgcct tgtggattaa
     2161 gcgccagcgg taaatggatg taatctggca ctttccagcc aaaaagctgg tacagcgaga
     2221 tttgccgcac cgtcggttcg atcaaatctg ccccgcggac aatttcgctc acgccctgga
     2281 agtgatcatc caccaccacc gccaggttat aggcaaacaa accatcacgg cgatgaataa
     2341 taaaatcttc ccgtgccagt ttttcgtcgg cgtgaataat gcctcgcagc agatcagtaa
     2401 actgggtgac cggatgctgc tggcggatac gtactgcggc gttgtctggt ccatgatgca
     2461 aatcccggca atgaccgtcg taaataccgc caatgctttg aatacgcgca cgcgtgcagg
     2521 tgcagtaata acttagtcct tgctcatgta accaggcgag tgcttcgcga taggcgtggt
     2581 gacgttgcga ttgccagaga acgtcgccgt cccagtacag gccgtaatgt tccagctggc
     2641 gcaggatagt ttctgcggca ccgggaactt cacgaggcgg gtcgatatct tcaatgcgta
     2701 ccagccagcg accttgccgg gcgcgagcct gcaaatagct gccaagcgca gcaatcagag
     2761 aaccaaaatg aagctcgccg gaaggagagg gggcgaagcg gccaatatag tgtgtgtctg
     2821 tcatctcttt gaacaaaaaa taaggcggga gcatttcccg cctgtggtaa acgtgatgga
     2881 acggctgtaa ttagccagcc atctgttttt cgcgaatttc agccagcgtt ttgcagtcga
     2941 tgcacagatc ggctgtcggg cgcgcttcca gacggcgaat accgatttca acaccgcagg
     3001 attcgcagta gccgaaatct tcgtcttcca cttttttcag cgtcttctcg atctttttga
     3061 tcagcttacg ctcgcgatcg cggttacgca gttcgaggct gaactcttct tcctgggctg
     3121 cacggtctac cgggtccggg aagttggctg cttcatcctg catatgtgta acggtgcgat
     3181 cgacttcatc cctgagttga ttacgccatg cttccagaat acgacggaag tgcgccagct
     3241 gggcttcatt catatactct tcgcccggct tctcctgata tggttccacc ccagcgatgg
     3301 cgagaatact cagggacgat gttttacggt tttgcccttc ttgcatgttg cttctcctta
     3361 acacgcacta tcgatcccca tgttcggggg aaaaatgagg ccgctataaa tagcagatgc
     3421 ttttccggat agcaattatc taaacgtaac acttgacaac tgtgtgagga aaagcgtatt
     3481 tgcgcacgcg accagaatgt aaattaacca gttacttact ctactacaat gtaaccggta
     3541 gtgatttttt aagagccatg ccttcggcag aaagttccgc tttgtaagcc agaatttcta
     3601 cccccctctg ttgagcttct gacaatagtt gcgcgtattt ctcatcgatg tggcgcgcgg
     3661 gtgaaaaccg tgtaatggct gaatgcagca cggcgaaaaa gataaccgca cgctggcctt
     3721 cagccgctac gctcatcaac tcccgaaggt gtttctgacc tcgttcagtg accgcatcgg
     3781 gaaaatatcc ctgttcgttc tccgctaacg taaccgattt cacttcaata tagcagtctg
     3841 gacgcgaatc cgcctgcaac ataaagtcaa tacggctgcg ttcggcgccg tattttactt
     3901 cgcttttcag cgagctatag cctgccagct ctgaaattga ttcattaagg atagcctctt
     3961 tcgtcaacct gttagcccaa agcgtattga cgcaaataat tgcgccgctc tggctttgag
     4021 ttaattccca ggtgtgtggg tatttccgtt tggtgttgtc tgaagtcgaa taccagacgg
     4081 tatcgccagg cgttgcacaa ccggtcatcg cacccgtatt cgggcagtgt agcgttaatt
     4141 cgcgaccatc gggtgtgatc acatcggcta aaaaacgttt gtaacgctga attagcgtcg
     4201 cgcgctgtag agggggagaa aattccattg cgacaatcct tgttattgcg ttagcgccca
     4261 gcgttttagc ggcgtgtagc gtgtgcgtcc acgggcaaac gaggaggcgt aaagggtgaa
     4321 ctccgtcacc gtatacgacc agttaaaacc tggcggcggg attgtcaccg cctcgctggc
     4381 gtcgcgcaat aaggtaatat gtggatgaaa cggacgattg ctttgaaaac aaccgctgcg
     4441 ggcagcctgt gagcggagca tattcgccag ctggattaag ccgcgaggcg gctgtcgcat
     4501 ccctaaccac accacacgcg aacgcagcca ttgaccggcg tcatcaagcg tgagtgtgaa
     4561 accaggttga cgaatccgtc cggctaaaag agaaagcgcc ttctctttct ctgcgctcac
     4621 ttcgcctaaa aatgccagag tcagatgcaa attatcggcg gcgaccggac gtcccgcctc
     4681 aggtgggaag tgtgtggcgc gccagtggat aatctgttcg cagatttctg caggtaagtc
     4741 gatagcaaag aacagccgtt gcggttcaga catgtgaggc actcggttat gaattaccgc
     4801 gatgctacaa tgtggcgcga agaatgttaa ccctctggag cgttttgtgt cgtcgttgcc
     4861 cgttgctgcc gtcttacctg agttacttgc tgccctcgat ggtgcatcgc aggtgttatt
     4921 aagtgcgccg accggggccg ggaaatcaac ctggctgccg ctgcaactgc tggcgcatcc
     4981 cggcattaac gggaaaatta tcctgctgga gccgcgtcgt ctggcggcgc gtaacgttgc
     5041 gcaacgactg gcggagctgc ttaacgaaaa gccaggcgat accgttggct accggatgcg
     5101 tgcgcaaaac tgcgtcgggc cgaatacccg cctggaagta gttaccgaag gcgtgctgac
     5161 gcggatgatc cagcgtgacc cggaactgag cggtgttgga ttggtgatcc ttgatgaatt
     5221 tcatgagcgc agcttgcagg cggatttggc gttggcgctg ttactcgatg tgcaacaagg
     5281 tctgcgtgat gaccttaaac tgctgattat gtcggctacg ctggacaacg accgcttgca
     5341 gcaaacgctg ccagaagcgc ccgtcgtcat ctcagaaggg cgctcgtttc cggttgaacg
     5401 ccgttatttg ccgctgcccg ctcatcagcg ttttgacgaa gccgtcgcgg ttgctaccgc
     5461 cgaaatgctg cgtcaggaaa gtggatcatt actgttattt ttacctggcg tcggagaaat
     5521 tcagcgtgtg caggaacaac tgacttcacg catcggcagt gatgtgttgc tctgcccgct
     5581 gtatggcgcg ttgtcgctga acgatcagcg aaaagcgatc ctcccggcac cgcaagggat
     5641 gcgtaaagtg gtgctggcga ccaatattgc tgaaacaagt ttaaccatcg aaggtattcg
     5701 tctggtggtg gattgtgccc aggaaagggt ggcgcgtttt gatccgcgta cagggcttac
     5761 gcggttgatt actcaacgcg ttagtcaggc gtcgatgacg caacgtgccg ggcgtgccgg
     5821 gcgtctggag ccgggtatct gcctgcattt aatcgccaaa gaacaagcag aacgcgctgc
     5881 cgcacaaagt gaaccggaga tcttacaaag cgatctttcc ggtttgctga tggaattact
     5941 gcaatgggga tgcagcgatc cggcgcagat gagctggatg gatcaaccgc caacggtgaa
     6001 tctactggcc gcgaaacgcc tgttacggat gctgggggcg ctggacggtg aacggcttag
     6061 tgcgcaaggg caaaaaatgg caacgctggg taacgatccg cgtttagcgg caatgctggt
     6121 tagcgcgaag aacgacgacg aagctgctac cgcggcaaaa attgccgcca ttctcgaaga
     6181 gccgccacgg atgggcaata gtgacctggg cgtggcgttt tcgcgcaatc aacccgcctg
     6241 gcagcaacgt agtcagcaac tgttaaaacg tttaaacgta cgcggcggtg aggcagacag
     6301 ttcgcttatc gcgccgctac ttgccggagc gtatgccgat cgtattgctc gccgccgtgg
     6361 gcaagaggga cgctatcaac tggcgaacgg catgggggcg atgctcgatg ccgacgatgc
     6421 gctaagccgc cacgaatggt tgatcgcacc gttattattg cagggcagcg cctcgccgga
     6481 tgcgcggatt ttactggcgc tgccggtcga tattgatgag ttagtacaac gctgcccgca
     6541 gctggtacag cagtctgaca ctgtggagtg ggatgacgcg caaggtacgc tgaaagcctg
     6601 gcgtcggctg caaattggtc agttgatggt aaaagtgcaa ccgctggcga aaccctcgga
     6661 agacgagttg catcaggcga tgcttaacgg cattcgtgat aaaggtttaa gcgtgctcaa
     6721 ctggacggcg gaagcggaac agctacgctt gcgtttgtta tgcgccgcaa agtggttgcc
     6781 ggaatatgac tggccagcgg ttgatgatga aagtttgttg gcgacgctgg aaacgtggct
     6841 actgccgcat atgacaggcg tacattcgct acgcggcctg aaatcactcg atatttatca
     6901 ggcactgcgt ggattacttg attgggtaat gcagcaacgt ctggatagtg aattgcctgc
     6961 gcattacact gtgccgacgg gaagccggat cgccattcgt taccatgaag ataacccgcc
     7021 cgcgctggcg gtgagaatgc aggagatgtt tggtgaggcc actaatccga ctatcgccca
     7081 ggggcgcgta ccgttggtgc tggagttgct ttcccctgcc caaaggccgc tgcaaatcac
     7141 gcgtgatttg agcgccttct ggaaaggagc gtaccgtgag gtgcaaaaag agatgaaagg
     7201 gcgttatccc aaacatgtct ggccggacga cccggcaaac accgcaccga cgcgacgaac
     7261 gaaaaagtat tcgtgaagac caaagcaaat ttgagagata tcttcttctg tcttgcaaca
     7321 gaagaacaga aaatcgggct tttgcgcctg aatattgcgg agaaaaagca tggccgggaa
     7381 tgaccgcgag ccaattggac gcaaagggaa accgacgcgt ccggtcaaac aaaaggtaag
     7441 ccgtcgtcgt tacgaagatg acgatgatta cgacgattat gatgactatg aggatgaaga
     7501 accgatgccg cgcaaaggta agggcaaagg gcgtaagcct cgtggcaaac gcggctggct
     7561 atggctgctg ctaaaactgg ctatcgtttt tgccgtgatg atcgccattt acggcgttta
     7621 tctcgatcaa aaaattcgca gccgtattga tggcaaggtc tggcaattgc ccgcggcagt
     7681 ttatggtcga atggtcaacc ttgagccaga catgaccatc agcaagaacg agatggtgaa
     7741 gctgctggag gcgacccagt atcgccaggt gtcgaaaatg acccgtcctg gtgagtttac
     7801 cgtacaggcc aacagcattg agatgatccg ccgtccgttt gatttcccgg acagcaaaga
     7861 agggcaggtg cgcgcgcgtc tgacctttga tggcgatcat ctggcgacga tcgtcaatat
     7921 ggagaacaac cgtcagttcg gtttcttccg tcttgatccg cgcctgatca ccatgatttc
     7981 ttcgccaaac ggtgagcagc gtctgtttgt gccgcgcagt ggtttcccgg atttgctggt
     8041 ggatactttg ctggcgacag aagaccgtca tttctacgag catgatggga tcagtctcta
     8101 ctcaatcgga cgtgcggtgc tggcaaacct gactgccgga cgcacggtgc agggggcgag
     8161 tacgctgacg caacagttgg ttaaaaacct gttcctctcc agcgagcgtt cttactggcg
     8221 taaagcgaac gaagcctaca tggcgctgat catggacgcg cgttacagca aagaccgtat
     8281 tcttgagctg tatatgaacg aggtgtatct cggtcagagc ggcgacaacg aaattcgcgg
     8341 cttcccgctg gcgagcttgt attactttgg tcgcccggta gaagaattga gcctcgatca
     8401 gcaggcgctg ttggtgggta tggtgaaagg ggcgtccatc tacaacccgt ggcgtaaccc
     8461 gaaactggcg ctggagcgac gtaacctggt actacgtttg ctgcaacagc aacaaattat
     8521 tgatcaagaa ctctacgaca tgctgagtgc gcgtccgctg ggtgttcagc cgcgcggtgg
     8581 ggtgatctct cctcagccag cctttatgca gctggtgcgt caggagctgc aggcgaaatt
     8641 aggcgataag gtaaaagatc tctccggcgt gaagatcttc actacctttg actcggtggc
     8701 ccaggacgcg gcagaaaaag ccgccgtgga aggcattccg gcactgaaga aacagcgtaa
     8761 gttgagcgat cttgaaaccg cgattgtggt tgttgaccgc tttagcggtg aagttcgtgc
     8821 gatggtcggt ggttctgagc cgcagtttgc gggctacaac cgtgcgatgc aggcgcgtcg
     8881 ttcgattggt tcccttgcaa aaccagcgac ttatctaacg gccttaagcc agccgaaaat
     8941 ctatcgtttg aatacgtgga ttgccgatgc gccaatcgcg ctgcgtcagc cgaatggcca
     9001 ggtctggtca ccgcagaacg atgatcgtcg ttatagcgaa agtggcaggg tgatgctggt
     9061 agatgcgttg acccgttcga tgaacgtgcc gacggtgaat ctgggcatgg cgttggggct
     9121 gcctgcggtt acggagacct ggattaaact gggtgtgccg aaagatcaac tgaatccagt
     9181 tccagcaatg ttgttggggg cgttgaactt aacgccaatc gaagtggcgc aggccttcca
     9241 gactatcgcc agcggtggta atcgtgctcc gctttccgcg ctacgttcag tcatcgcaga
     9301 agacggcaag gtgttgtatc agagcttccc gcaggcggaa cgcgctgttc cggcgcaggc
     9361 ggcgtatctg acactatgga ccatgcagca ggttgtacaa cgcggtacgg gtcgtcagct
     9421 tggggcgaaa tacccgaacc tgcatctggc agggaaaaca gggactacca acaataacgt
     9481 agatacctgg tttgcgggca ttgatgggag cacggtgacc atcacctggg tcggccgtga
     9541 taacaaccag ccgaccaaac tgtatggtgc cagcggggca atgtcgattt atcagcgtta
     9601 tctggctaac cagacgccaa cgccgctgaa tcttgttccg ccagaagata ttgcagatat
     9661 gggcgtggac tacgacggca actttgtttg cagcggtggc atgcgtgtct tgccggtctg
     9721 gaccagcgat ccgcaatcgc tgtgccagca gagtgagatg cagcagcaac cgtcaggcaa
     9781 tccgtttgat cagtcttctc agccgcagca acagccgcaa cagcaacctg ctcagcaaga
     9841 gcagaaagac agcgacggtg tagccggttg gattaaagat atgtttggga gtaattaacg
     9901 tctaagcgtg atataccgga tggcgagctg ccatccggta aaataacatc ccctccaaag
     9961 tattaaccct ttcttttcat ctgattgttt attgaccctt caggaatgcg cagattgcgt
    10021 accgcttgcg aacccgccag cgtttcgaat attatctcat ctttataata atcattctcg
    10081 tttacgttat cattcacttt acaccagaga aataccaatg gcaccttcca aaactgctca
    10141 gccaaaacac tcactgcgta aaatcgcagt tgtagtagcc acagcggtta gcggcatgtc
    10201 tgtttatgca caggcagcgg ttgaaccgaa agaagacact atcaccgtta ccgctgcacc
    10261 tgcgccgcaa gaaagcgcat gggggccggc tgcaactatt gcggcgcgac agtcagctac
    10321 cggcactaaa accgatacgc cgattcaaaa agtacctcaa tctatttctg tcgtcaccgc
    10381 tgaagagatg gcgctgcatc agcctaagtc ggtgaaagaa gcgcttagct atacgccggg
    10441 tgtctctgtt ggtacgcgtg gcgcatccaa cacctatgac cacctgatca ttcgcggttt
    10501 tgcggcagaa ggccaaagcc agaataacta tctgaatggc ctgaagttgc agggcaactt
    10561 ctataacgat gcggtcatcg acccgtatat gctggaacgt gctgaaatta tgcgtggccc
    10621 ggtttccgtg ctttacggta aaagcagtcc tggcggtctg ttgaatatgg tcagcaagcg
    10681 tccgaccacc gaaccgctga aagaagttca gtttaaagcc ggtactgaca gcctgttcca
    10741 gactggtttt gactttagcg atgcgctgga tgatgacggc gtttattctt atcgcctgac
    10801 cggtcttgcg tgttctgcca atgcccagca gaaagggtca gaagagcagc gttatgctat
    10861 tgcaccggcg ttcacctagc gtccggatga taaaaccaat ttcaccttcc tttcttactt
    10921 ccagaacgag ccggaaactg gttattacgg ctggttgccg aaagagggaa ccgttgagcc
    10981 gctgccgaac ggtaagcgtc tgccgacaga ctttaacgaa ggggcgaaga acaacaccta
    11041 ttctcgtaac gagaagatgg tcggctacag cttcgatcac gaatttaacg atacctttac
    11101 tgtgcgtcag aacctgcgct ttgctgaaaa caaaacctcg caaaacagcg tttatggtta
    11161 cggcgtctgc tccgatccgg cgaatgctta cagcaaacag tgtgcggcat tagcgccagc
    11221 ggataaaggc cattatctgg cacgtaaata cgtcgttgat gatgagaagc tgcaaaactt
    11281 ctccgttgat acccagttgc agagcaagtt tgccactggc gatatcgacc acactctgct
    11341 gaccggtgtc gactttatgc gtatgcgtaa tgacatcaac gcctggtttg gttacgacga
    11401 ctctgtgcca ctgctcgatc tgtacaatcc ggtaaatacc gatttcgact tcaatgcgaa
    11461 agacccggat aactccggtc cgtaccgcat tctgaataag cagaagcaaa cgggcgttta
    11521 tgttcaggat caggcgcagt gggataaagt gctggtcacc ctgggcggtc gttacgactg
    11581 ggcagatcaa gaatctctta accgcgtagc gggaacgacc gataaacgtg atgacaaaca
    11641 gtttacctgg cgtggtggtg ttaactacct gtttgataat ggtgtaacac catacttcag
    11701 ctatagcgaa tcgtttgaac cttcttcgca agttgggaag gatggtaata ttttcgcacc
    11761 gtctaaaggt aagcagtatg aagtcggcgt gaaatatgta ccggaagatc gtccgattgt
    11821 agttactggt gccgtgtata atcttactaa aaccaacaac ctgatggcgg accctgaggg
    11881 ttccttcttc tcggttgaag gtggcgagat ccgcgctcgt ggcgtagaaa ttgaagcgaa
    11941 agcggcgctg tcggcgagtg ttaacgtagt cggttcttat acttacactg atgcggaata
    12001 cactaccgat actacctaca aaggcaatac gcctgcacag gtgccaaaac acatggcttc
    12061 gctgtgggct gactatacct tctttgacgg tccgctttca ggtctgacgc tgggcaccgg
    12121 tggtcgttat actggctcca gctatggtga tccggctaac tcctttaaag tgggaagtta
    12181 tacggtcgtg gatgcgttag tgcgttatga tctggcgcga gtcggcatgg cgggctccaa
    12241 cgtggcgctg catgtcaaca acctgtttga tcgtgaatac gtcgccagct gctttaacac
    12301 ttatggttgc ttctggggcg cagaacgtca ggtcgttgca accgcaacct tccgtttcta
    12361 atttctcttt tggggcacgg atttccgtgc ccgtttcaca agttggctgt tatgcaggaa
    12421 tacacgaatc attccgatac cacttttgca ctgcgtaata tctcctttcg tgtgcccggg
    12481 cgcacgcttt tgcatccgtt gtcgttaacc tttcctgccg ggaaagtgac cggtctgatt
    12541 ggtcacaacg gttctggtaa atccactctg ctcaaaatgc ttggtcgtca ccagccaccg
    12601 tcggaagggg agattcttct tgatgcccaa ccgctggaaa gctggagcag caaagcgttt
    12661 gcccgcaaag tggcttattt accgcagcag cttcctccgg cagaagggat gaccgtgcgt
    12721 gaactggtgg cgattggtcg ttacccgtgg catggcgcac tagggcgctt tggtgctgca
    12781 gagcgcgaaa aggtcgagga agctatctcg ctggttggct taaaaccgct ggcgcatcgg
    12841 ctggtcgata gcctctctgg cggcgaacgt caacgggcgt ggatcgctat gctggtggcg
    12901 caggatagcc gctgtctgtt gctcgacgaa ccgacctcgg cgctggatat cgcccaccag
    12961 gttgatgtac tggcgctggt gcatcgttta agtcaggagc gaggcctgac ggtcattgcc
    13021 gtgctgcacg atatcaatat ggcggcgcgc tactgtgatt atctggtcgc cctgcgcggc
    13081 ggtgaaatga ttgctcaggg aacgcctgcg gaaattatgc gcggcgaaac cctcgaaatg
    13141 atttatggca tcccgatggg tattctgccg catccggcgg gtgctgcacc tgtgagtttt
    13201 gtttattgat gagcggctta cctcttattt cgcgccgccg actgttaacg gcgatggcgc
    13261 tttctccgtt gttatggcag atgaataccg cccacgcggc agttattgat cccaatcgta
    13321 ttgtggcact ggagtggttg ccggtggagt tactgctggc gctcggcatc gtgccttacg
    13381 gcgtggcgga caccatcaac tatcgcctgt gggtcagcga accgccattg ccggactcag
    13441 tgattgacgt tggtttgcgt acagaaccca accttgaact gctgaccgaa atgaaaccgt
    13501 cgtttatggt ctggtctgca ggatatggcc cttcaccaga aatgctggca cgtattgcgc
    13561 cggggcgcgg atttaacttc agcgacggca aacagccact ggcgatggcg cgtaaatcac
    13621 tgacggaaat ggcagattta cttaacctgc aaagcgcagc ggaaacgcat ttagcgcact
    13681 atgaagactt tatccgcagc atgaaacccc gctttgtgaa gcgtggtgca cgcccgttat
    13741 tgctgacgac gcttatcgat ccgcgccata tgttagtctt cggtccaaac agcttgttcc
    13801 aggaaattct cgatgagtac ggcatcccaa atgcctggca aggggaaacc aacttctggg
    13861 gcagtaccgc cgtcagtatc gatcgtctgg cagtgtataa agacgttgat gtgctctgtt
    13921 ttgatcacga caacagcaaa gacatggatg cgctaatggc aaccccgctg tggcaggcca
    13981 tgccgtttgt ccgcgccgga cgctttcagc gcgttccggc ggtctggttc tatggtgcga
    14041 cgctctcggc aatgcacttt gtgcgtattc tggataacgc catcggaggt aaagcgtgag
    14101 taaacgaatt gcgctttttc cggtgttatt gctggcgctg ctggtggttg ctgctgcggc
    14161 gttgacctgg atgaacttct cgcaggcgct gccgcgcagc cagtgggcgc aggccgcctg
    14221 gtcgccggat attgacgtca ttgagcagat gatttttcac tacagcttgt tgccgcgtct
    14281 ggcgatttcg ctgctggtgg gcgcgggcct ggggctggtg ggcgtgctgt ttcagcaagt
    14341 gctgcgtaac ccgctggcgg aaccgacgac gttgggtgtt gcaacaggcg cgcaactggg
    14401 gattaccgtc accacgctct gggcgatccc cggcgcgatg gcgagccagt ttgctgcgct
    14461 ggcaggcgcg tgtgttgttg gcttaatcgt ctttggcgtc gcgtggggga aacggctttc
    14521 gccggtaacg ctgatcctcg cggggctggt agtgagcctt tattgcggcg caatcaatca
    14581 gttactggtt atcttccatc atgaccaact gcaaagcatg ttcctgtgga gcaccggaac
    14641 gctgacgcaa accgactggg gcggcgttga gcgtttatgg ccgcagctgc tgggcggcgt
    14701 gatgctgacg ttattgctac ttcgcccgtt aactctgatg gggcttgatg atggcgtggc
    14761 gcgcaatctc gggctggcct tgtcgctcgc tcgtctggcg gcgttgtcgc tggcgattgt
    14821 catcagtgcg ctgctggtga acgcggtggg gattatcggc tttatcggtt tgttcgcgcc
    14881 actgctggtg aaaatgctgg gggcgcggcg tttgttgcca cggttgatgc tggcatcgct
    14941 gattggtgcg ttaattctgt ggctttctga tcaaatcatc ctctggctga ctcgcgtgtg
    15001 gatggaagtg tccaccggtt cggtcactgc gttgatcggt gcgccgctgc tactgtggtt
    15061 gctgccgcgt ttacgcagca ttagcgcgcc ggatatgaag gtgaacgatc gtgtcgcgac
    15121 tgaacgccaa cacgtgctgg cgtttgccct cgcgggcggc gtgctgctgt tgatggctgt
    15181 ggtggtggcg ctgtcgtttg gtcgtgatgc gcacggctgg acgtgggcga gcggggcgtt
    15241 gctcgaggat ttgatgccct ggcgctggcc gcgaattatg gctgcgctgt ttgcgggcgt
    15301 catgctggcg gtggcgggct gtattattca gcggctgacc ggaaacccga tggcaagccc
    15361 ggaagtgctg gggattagct ccggcgcggc gtttggcgtg gtgttaatgc tgtttctggt
    15421 gccgggtaat gcctttggct ggctgttacc cgcaggcagt ctcggggcgg cggtgacgct
    15481 gttgatcatt atgatcgccg ccggacgtgg tggattttcc ccacaccgca tgttactggc
    15541 ggggatggcg ttaagtaccg cgttcaccat gcttttgatg atgttgcagg caagtggtga
    15601 cccgcgaatg gcgcaagttc tgacctggat ttccggttcg acctacaacg cgaccgatgc
    15661 gcaggtctgg cgtaccggaa ttgtgatggt gattttgctg gcgattaccc cgctgtgccg
    15721 ccgctggctg accattttac cgctgggtgg tgataccgca cgagccgtgg ggatggcgct
    15781 gacgccgacg cgaattgcgc tgctgctgtt agcggcttgc ctgacggcga ccgcgacaat
    15841 gaccattgga ccgttgagtt ttgttggttt aatggcaccg catattgcgc ggatgatggg
    15901 ctttcgacgg acgatgccac acatcgtaat ttcggcgctg gtgggtggtt tactgctggt
    15961 gttcgctgac tggtgtgggc ggatggtgct gtttccattc cagatccccg cggggctgct
    16021 gtcgaccttt atcggcgcgc catattttat ctatttgttg agaaagcaga gccgttaata
    16081 gaatgcctcg cccggtgggc gaggcaaaat cattacaact tcgcaaacac ccgatgtgca
    16141 gcatcgatgg tgttattgat atcttccatg ctatgcgcca cggacataaa gcccgcttca
    16201 aacgctgacg gtgccaggta aacaccttcg tccagcatca tatggaagaa acgcttaaag
    16261 cgttccacgt cacaggccat cacatcctga tagcacgtca cggactcggc gtcggtaaag
    16321 aaaataccga acatgccgcc aacgtggttt accaccagcg gaattccggc ttcttctgcc
    16381 gcttcccgca gaccttctgc cagacgtgtt gtcagctcat ccagcgtttc gtgaacgccc
    16441 ggctgcgcga cttcattcag acaggcgaaa ccggctgcca tcgcaatcgg gttaccggaa
    16501 agcgtacccg cctgatatac cggaccggtc ggggccagcg catccattac atcacgacga
    16561 ccaccgaatg cgcctaccgg cattccaccg ccgatgattt tgccgaggca ggtgagatcc
    16621 ggttccacac cgtaataatc ctgtgcgcca gctagcgcta cgcggaaacc ggtcatcact
    16681 tcatcgatga tcagcaacgc gccaaattcg tcgcacagcg cgcgcagacc tggcaggaac
    16741 tctggcagcg gtggaacgca gttcatattg cctgccaccg gctcgacgat aatacaggca
    16801 atctcttgcg ggtattgctc aaacgcggcg cgtacagagg ccagatcgtt ataagtacag
    16861 gttaaggtat gtttggcgaa atctgccgga acgcccggcg aatttggctg gcctaacgtg
    16921 agtgcgccag aaccggcttt caccagcagg cagtcagcgt gaccgtggta acaaccttca
    16981 aatttaataa ttttgtcgcg accggtaaaa ccacgggcca ggcggatggc gctcatcgtc
    17041 gcttcagtgc cggagttcac catgcgcacc atatccatgg tcgggaccag ttcggtcacc
    17101 agttgcgcca ttttcacttc catttcggtt ggtgcaccaa agcttaaacc acgctcggcg
    17161 gcttcaatca cggcattgcg gatcgccgga tggttatggc ccagcaccat cggcccccag
    17221 gaaccgacat aatcgatata ggctttgcca tcaacatcgt acagataagc gccgtccgct
    17281 ttttcgataa acagtggagt gccgcccacg ccagtaaagg cgcgaacagg ggagttcaca
    17341 ccgccaggga tcagctcgcg cgctgcgctg taaagatttt cagacttact catggagggt
    17401 tcctgattcg tagaaaaagt gaatggctgc tattctatgt tattcataac aagttaaata
    17461 ctcgtcaaac atcaggctgc ttgtactggt gcaatcctga tttcttagag tataaaagtt
    17521 ttgtgcattt gaaacattac gctttgcaaa ggattttcat ggaacgtgcg agtaaaatgc
    17581 cgtcatctta tttgtatgac caataagtga tcattggatg aaaactgata ctccctcttt
    17641 agaaacaccg caggccgcgc gcctgcgacg cagacaactg attcgccaac ttcttgagcg
    17701 cgataaaacc ccgttagcca ttttgtttat ggcggcagtc gtcggcacgc ttgtcgggct
    17761 ggctgcggtt gcttttgaca aaggtgtcgc ctggttgcag aaccaacgta tgggggcact
    17821 ggtacatact gctgataatt atccgctact gttaaccgtc gcttttctct gttcggcggt
    17881 gctggcgatg ttcggctact ttttggtgcg caaatacgcg cccgaagcag gggggtcggg
    17941 gatcccggaa attgaagggg cgctggaaga tcaacgtccc gttcgctggt ggcgtgtgtt
    18001 gccggtgaaa ttctttggcg ggctggggac actcggcgga ggcatggtgc tggggcgcga
    18061 agggccaacc gtgcagatcg gcggtaatat cgggcggatg gtactcgatg ttttccgcct
    18121 gaaaggtgac gaagctcgtc atacgctact ggcaaccggt gctgcggcgg ggctggctgc
    18181 ggcctttaac gcgccgctgg cgggtatttt gtttattatc gaagagatgc gtccgcagtt
    18241 tcgctatacg ttaatttcga ttaaagcggt atttattggc gtcattatgt cgaccattat
    18301 gtaccggatt tttaatcatg aagttgcgtt gattgacgtc ggtaaacttt ctgacgcgcc
    18361 gcttaatacg ctgtggcttt atctgatcct cggtattatt tttggcattt tcggccctat
    18421 ttttaataaa tgggtgctgg gaatgcagga tttgctgcac cgtgtgcacg gcggcaatat
    18481 taccaaatgg gtagtgatgg gcggtgcgat tggcggtctg tgtggattgc tggggtttgt
    18541 ggcaccagca acgtcgggcg gcggctttaa cctgattcct atcgctaccg cagggaattt
    18601 cagcatggga atgctggtgt ttatcttcgt cgcgcgggtc attaccactt tactctgctt
    18661 ctcttccggc gcgccgggcg gtatttttgc cccgatgctg gcgctgggta ctgtgctggg
    18721 aaccgctttc ggaatggttg ccgttgagtt gtttccgcaa tatcaccttg aggcggggac
    18781 gtttgctatt gccggaatgg gggcgttact ggcggcatct attcgcgcgc cgttaacggg
    18841 gatcattctg gttctggaaa tgaccgataa ctaccagctc attttgccaa tgattattac
    18901 cggtcttggc gcaacactat tagcacaatt taccggtggg aaaccgctat actcggcgat
    18961 tcttgcgcgt acgctggcaa aacaggaagc tgagcaactg gcgcgaagca aggccgcatc
    19021 agcccgcgag aatacttgaa cgaaatacca gggtattaga taatggcgat tattattggg
    19081 ttagaatttg cccaattgcc gatgtcgttt ggagcaaaat atgagtgatg acgtagcact
    19141 gccgctggag tttaccgacg cagcagccaa caaagttaaa agcctgatcg ctgacgaaga
    19201 taacccgaat ctgaaattac gcgtgtatat caccggtggc ggttgcagcg gcttccagta
    19261 tggtttcacc tttgatgatc aggtgaacga aggcgatatg accatcgaaa aacagggcgt
    19321 tggcctggtg gttgatccga tgagcctgca atatctggtc ggcggttccg ttgactatac
    19381 cgaaggtctg gaaggttctc gtttcatcgt gaccaacccg aacgcgaaaa gcacctgcgg
    19441 ttgcggttct tccttcagca tataatctgt tgttcatgtg ccggataaac tatccggcac
    19501 atcttctctc ttcaacgccc attctcatcc agcgcaaacg tcggtagctt aagatgccaa
    19561 cgaatagccg ccagccgaat caatagcgtc acgaccatgc ccatcatact ggctgtttcc
    19621 agtggtacgg aaaatgtgta ataagccgta gcgtggacaa taccgccgat aatacaggca
    19681 gttgcgtaga tttctgtacg taaaatcatg gggatttcgc gggccagtac atcacgaatg
    19741 atcccgccgc caacgccagt aatgacgccc atacaaaccg cgattaacgg accggcttcc
    19801 gcattaaagg ctttattcac gccaatgccg acaaacaccg ccagaccaac ggcgtccagc
    19861 accggcaaca tccattttgg taagcgtctt ggctggcgca ccagcacgat ggtcagcatg
    19921 ctggtgacca ttgcaacgac cagatcggtg ggatctttca cccaaaatac cgggccgtga
    19981 tccagcgcca tgtcgcgaat tgttccgccg cctactgcgg taaccacgcc caataccaga
    20041 acgccaaaag ggtccatacg caattttccg gctaacaaaa cgccagagat agcaaatacc
    20101 gctgtgccga ctatatccag ccaatagacg agcattgttc aatccccact gagcacctga
    20161 aaaggtcagg cgctaatcta cctgtgaaag cgcattacag agctgttgtg cagcgaggat
    20221 aatacgtggg cttgcacgtt caaaccagtc actcgtgaga ggaataacgg gaattttgag
    20281 ctgttcaccc cagtattgtt tgattttagg aatttggtcc ggtccgcctg taatgacaat
    20341 cgcctgtggc gagcgtgcta acacctgttc gcggctaact tgcggccagg gaacccggct
    20401 gtctttaaag atgttttctc cgccacaaac ttcgagtacc tggttctgaa tcgactcttt
    20461 tccactggta aatggcggat taatgccgaa ttgcagaaaa acacgttttt taggtttatc
    20521 agcatattgc gctttcaatt gcgcgtactg atccagcagg gattgcgcgg cttgttcggc
    20581 cttatccggt tgcggactcc agggggccag ttgacgtaac acattggcaa tttgttcaat
    20641 gcttgtcgca tcgacccaca tcacttttat tcccagcgaa gccagctggt caacctgccg
    20701 ctcggcatta cctccacgcc aggcaatcac cagatcgggt ttcagcgcga caatgcgttc
    20761 cagattcatc ccctgccagg tggaaacctg ctcaatcttt tgcgcttgtg gaggatagtc
    20821 ggaatagctg ctgaccccaa ccggcgtgat cccggcggca aaggcaagtt cagtgttggc
    20881 gggagaaagc gtgatgacgc gcggcgcggc attgagccac agcggcgcga gaaaagacag
    20941 ggcgaccagc gccctgaaca gtgacttagc catgtgccag tttctgcacc agtgactcaa
    21001 ccatcaggct ggactgttta gcggcaacag ccaggaactc atcgaagcta agatgagact
    21061 gttgatcggc cacgtcggaa atggcgcgta ctacgacaaa cgggacgttg aaattgtggc
    21121 agacatgggc gattgccgtc gcttccatct ctacagcaat ggcctgtggg aagttgtggc
    21181 ggattttcgc cagaccaaca gaaccgttga tgaaagcgtc gccgctaaca atcaggccac
    21241 gtacagcgtt aagattcagt tcggcaatgc aggcctcagc ggcagcgatc agtttatcgt
    21301 cagctttgaa gcctgccggg cagcctggta actgaccgta ttcataacca aatgccgtga
    21361 catccgcgtc gtgataacgc gcttcgtccg agacaacgat atcgcccact ttcagcgttg
    21421 gtgccaggcc accggcagaa ccggtgttaa taatcacatc tggcttgcag tgttccaaca
    21481 gcaaagtggc acccagcgct gcagcgactt taccgatgcc cgatttcaga agcgcaacct
    21541 cggttccatt cagttggccg gtatagattt cgcaaccgcc cagactgata gtttgacggt
    21601 tttcgatttt gtcacgcagc agcgtaactt cttcttccat tgcaccaatg atgccgattt
    21661 tcatagattt actcgcgata agcccgattt gaaggcatag tttaccatgc gcttacgggg
    21721 aagcgtattt ctcacgcggg agaggacatg acacagattg atttccgaaa aaaaataaac
    21781 tggcatcgtc gttaccgttc accgcagggc gttaaaaccg aacatgagat tctgcggatc
    21841 ttcgagagcg atcgcgggcg tatcatcaac tctccggcaa ttcgtcgtct gcaacaaaag
    21901 acccaggttt ttccactgga gcgcaatgcc gccgtgcgca cgcgtcttac ccactcgatg
    21961 gaagtccagc aggtggggcg ctacatcgcc aaagaaattt taagccgtct gaaagagctt
    22021 aaattactgg aggcgtatgg tctcgatgaa ctgaccgggc cctttgaaag cattgttgag
    22081 atgtcatgcc tgatgcacga tatcggcaat ccgccgtttg gtcattttgg cgaagcggcg
    22141 ataaatgact ggtttcgcca gcgtttgtac ccggaagatg tcgaaagcca gcctctgact
    22201 gacgatcgct gcagcgtggc ggcactacgt ttacgggacg gggaagaacc gcttaacgcg
    22261 ctgcggcgca agattcgtca ggacttatgt cattttgaag gtaacgcaca gggtattcgt
    22321 ttggtgcata cattgatgcg gatgaatctc acctgggcac aggttggcgg tattttaaaa
    22381 tatacccgtc cggcgtggtg gcgtgttgaa acgcctgaga cacatcacta tttaatgaaa
    22441 aagccgggct attatctttc cgaagaagcc tatattgccc ggttgcgtaa agaacttaat
    22501 ttggcgcttt acagtcgttt tccgttaacg tggattatgg aagctgccga cgacatctcc
    22561 tattgtgtgg cagaccttga agatgcggta gagaaaagga tatttaccgt tgagcagctt
    22621 tatcatcatt tgcacgaggc gtggggccag catgagaaag gttcgctctt ttcgctggtg
    22681 gttgaaaatg cctgggaaaa atcacgctca aatagtttaa gccgcagtac ggaagatcag
    22741 ttttttatgt atttacgggt aaacacccta aataaactgg taccctacgc ggcacaacga
    22801 tttattgata atctgcctgc gattttcgcc ggaacgttta atcatgcatt attggaagat
    22861 gccagcgaat gcagcgatct tcttaagcta tataaaaatg tcgctgtaaa acatgtgttt
    22921 agccatccag atgtcgagca gcttgaattg cagggctatc gggtcattag cggattatta
    22981 gagatttatc gtcctttatt aaacctgccg ttatcagact ttactgaact ggtagaaaaa
    23041 gaacgggtga aacgtttccc tattgaaacg cgcttattcc acaaactctc gacgcgccat
    23101 cggctggcct atgtcgaggc tgtcagtaaa ttaccgtcag attctcctga gtttccgcta
    23161 tgggaatatt attaccgttg ccgcctgctg caggattata tcagcggtat gaccgacctc
    23221 tatgcgtggg atgaataccg acgtctgatg gccgtagaac aataaccagg cttttgtaaa
    23281 gacgaacaat aaatttttac cttttgcaga aactttagtt cggaacttcg cgctataaaa
    23341 cgaatctgaa gaacacagca attttgcgtt atctgttaat cgagactgaa atacatgaaa
    23401 aaaaccacat tagcactgag tgcactggct ctgagtttag gtttggcgtt atctccgctc
    23461 tctgcaacgg cggctgagac ttcttcagca acgacagccc agcagatgcc aagccttgca
    23521 ccgatgctcg aaaaggtgat gccttcagtg gtcagcatta acgtagaagg tagcacaacc
    23581 gttaatacgc cgcgtatgcc gcgtaatttc cagcagttct tcggtgatga ttctccgttc
    23641 tgtcaggagg gttctccgtt ccagagctct ccgttctgcc aggggggcct gggcggtaat
    23701 ggcggcggcc agcaacagaa attcatggcg ctgggttccg gcgttatcat tgatgccgat
    23761 aaaggctatg tcgtcaccaa caaccacgtt gttgataacg cgacggtgat taaagtccaa
    23821 ctgagcgatg gtcgtaagtt cgacgcgaag atggttggca aagatccgcg ctctgatatc
    23881 gcgctgatcc agatccagaa cccgaaaaac ctgaccgcaa ttaagatggc ggattctgat
    23941 gcgctgcgcg tgggtgatta caccgtagca attggtaacc cgttcggcct gggcgagaca
    24001 gtaacttccg ggattgtctc tgcgctggga cgtagcggcc tgaacgctga aaactacgaa
    24061 aacttcatcc agactgatgc agcgatcaac cggggtaact caggtggtgc gttggttaac
    24121 ctgaacggcg aactgattgg tatcaacacc gcgattctcg caccggacgg cggaaacatc
    24181 ggtatcggtt ttgctatccc gagcaacatg gtgaaaaacc tgacctcgca gatggtggaa
    24241 tacggccagg tgaaacgtgg tgagttgggt attatgggca ctgagctgaa ctccgatctg
    24301 gcgaaagcga tgaaagttga cgctcagcgc ggtgctttcg taagccaggt tctgccgaat
    24361 tcttccgccg cgaaagcggg cattaaagcg ggtgatgtga tcacctcact gaacggtaag
    24421 ccaatcagca gctttgccgc actgcgtgct caggtgggca ctatgccggt aggtagcaaa
    24481 ctgaccctgg gcttactgcg cgacgggaag caggttaacg tgaacctgga acttcagcag
    24541 agcagccaga atcaggttga ttccagcacc atcttcaacg gcattgaagg cgctgagatg
    24601 agcaacaaag gcaaagatca gggcgtggtg gtgaacaacg tgaaaacggg cactccggct
    24661 gcgcagatcg gcctgaagaa aggtgatgtg attattggcg cgaaccagca ggcagtgaaa
    24721 aacatcgctg aactgcgtaa agttctcgac agcaaaccgt ctgtgctggc actgaacatt
    24781 cagcgcggcg acagcaccat ctacctgtta atgcagtaat ctccctcaac cccttcctga
    24841 aaacgggaag gggttctcct tataatctgt gaacttcacc acaacttcat acatcttcat
    24901 catcctttgg gcatttgcac aatgccgtac gttacgtact tccttatgct aagccgtgca
    24961 taatggagga cttatggctg gctggcatct tgataccaaa atggcgcagg atatcgtggc
    25021 acgtaccatg cgcatcatcg ataccaatat caacgtaatg gatgcccgcg ggcgaattat
    25081 cggcagcggc gatcgtgagc gtattggtga attgcacgaa ggagcattgc tggtactttc
    25141 acaaggacga gtcgtcgata tcgatgacgc ggtggcacgt catctgcacg gtgtgcggca
    25201 ggggattaat ctaccgttac ggctggaagg tgaaattgtc ggcgtaattg gcctgacagg
    25261 tgaaccagag aatctgcgta aatatggcga actggtctgc atgacggctg aaatgatgct
    25321 ggaacagtcg cggttgatgc acctgttggc gcaggatagc cgtttgcggg aagaactggt
    25381 gatgaacctg attcaggctg aggagaatac tcccgcactt actgaatggg cgcaacggct
    25441 ggggatcgat ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca gcggtcagct
    25501 tggtgtggac agcgcaatgg cggagttaca gcaactgcaa aatgcgctga ctacgcccga
    25561 gcgtaataat ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga aaccggcgtt
    25621 gaactctttt gggcgctggg acgcagaaga tcatcgtaag cgagttgaac aactgattac
    25681 ccgcatgaaa gagtatggcc agttgcgttt tcgcgtttcg ctgggcaact attttaccgg
    25741 tcctggcagt attgcccgat cctatcgtac ggcgaaaacg acgatggtgg tggggaaaca
    25801 gcggatgcca gaaagtcgct gctattttta tcaggatctg atgttacctg tgttactcga
    25861 cagtttgcgt ggcgactggc aggccaacga actggcgcga ccgctggcgc ggctgaaagc
    25921 gatggacaat aacggcttgc tgcggcgaac gctggcggcg tggtttcgcc acaatgtgca
    25981 accgctggca acgtcaaagg cgttgtttat tcatcgtaat actctggaat atcggcttaa
    26041 tcgtatatcg gaactgaccg ggcttgattt gggcaatttt gatgacaggt tgctgctgta
    26101 tgtggcgtta caactggatg aagagcggta ggtcgattac aaaattacag ccgggtgaga
    26161 ctttacccgg ccacagaatg gcattattta cgcgtcagtt tttccagatc ggcttcaatc
    26221 tcgctgatct tattggttac tactgactcc aggtgacgta agtcgtcgag gatcttacgc
    26281 ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat caagctcatc aatgatatac
    26341 cgtagattcg ggctaatttc ctggacttct ttataaccct gacccacacc atcagcgacg
    26401 accgttttac gctgacgcgg gtatttaaac ttcacgctct tggcgaaaaa ctcgcctttg
    26461 tctttctgga aatagatttt cagaatatcg ttgttggctt cctgccggag gctgtaacga
    26521 tcaatttctt caggattggt aatacccaga cttttcagat tgtcgtacat agcgttacct
    26581 caaaatgagt cagtaaattg tgcttatttt agcatttggc ctggcccgcc cggctcgact
    26641 gtgatcgcag aaagcctttt tcttccagac ttctatcatg gcgtaaaaat caaaaaatta
    26701 cctgctttat tgtggtgata ttctatggga tggattagca catttatctt cattttgcga
    26761 attacatgac agtccgaaac aagatgaaaa agtatttaaa aaaaaggggg aggaggaaga
    26821 atgagtagga cgaattattc tgagccagaa agcaatcggg cagcgtcgtg ctgcccgtgt
    26881 acatactttt agtcgatggt acgcagcagt tcgttaatac caactttgcc gcgagttttc
    26941 gcgtcaactt tcttaacgat aactgcacag tagaggctgt atttgccatc tttcgacggc
    27001 agattacctg aaacaaccac agaccctgcc ggaacgcgac cgtagtggat ttcgccggtt
    27061 tcacggtcat aaatacgggt gctctgacca atgtatacgc ccatagagat gacggaacct
    27121 tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc cgatgaagca gttatcttca
    27181 ataatggtcg ggttagcctg cagcggctcc agcacgccgc cgatacccac gccaccggaa
    27241 aggtggacgt ttttaccaat ctgcgcacag gaaccgacgg tcgcccaggt atcaaccatg
    27301 gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac ggtgttacgg
    27361 gcaatgaacg cgccctgacg cactgccgct ggtggcacga cgcggaagcc ttctttctgg
    27421 aaacgtgctt catcgtagtt agcgaatttc atcggcactt tgtcgaagta gcggctttct
    27481 gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc ttttttcaac
    27541 cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc gccggaatcc
    27601 agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcatt ggctggcgtg
    27661 atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg cattgttaaa
    27721 ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc ctctgtcaac
    27781 cgctgatgca cttcctgctg caactcgtta ttaagcgcac gccggtcagc ggtggcaatt
    27841 atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcgccatg aagcgaaatt
    27901 cccagatcgg caaaaatttt cccgactcgc gccagcagtc caggttggtc gagggcgatc
    27961 agttcgagga acgatttgcg gtcggtatgg gtcggcaaaa acgtcacttc ggtttcaaca
    28021 gtaaaatggc gcaatttggc aggttggcga cgcggctgcg gtggctgcca gctactttgt
    28081 gtcagcacct gctccagacc aaaccgaata acctcatgac gatcggcgga cagcgggctg
    28141 ccatcgggtt ccagtacgat gaaggtatcc atcgccatac cgtcacgagt ggtgaaaatt
    28201 tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc ggcaaacaga
    28261 taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc ctgcgggcta
    28321 agcagtacca gcggtttgct taaatcatgc tgtaataaat ggcgtgcgtg ccaggccagt
    28381 tgatttgggc tatggcggac aaaatagtta gcgcgacagc gtgaccaaat ttggtgcagc
    28441 gcctcttcgt cgatgttatc catgcgcagt agtgccagcg catggagttg gtgatggcga
    28501 acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt ttcggtggca
    28561 aaatagagtt cgcgcaacag actttgcttc cagctattcc acagtgtttc gttggtggcg
    28621 caaatgtcag ccactgtcag gcataccaga tagcgcagac gattttccgt ttgaacttct
    28681 tcggcaaact gcttgatgac ttccgggtcc tgaatatcgc ggcgctgagc ggtcaccgac
    28741 atcagcaagt gctggcgaac cagccaggcg accaactgtg tttcgcgtga gttcagcccg
    28801 tggagttcgg caaaatgcac tacatcctga gcaccgagaa tggagtggtc gccgccgcgt
    28861 cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga cggcaggcgc
    28921 ggccagacgt ccacacacaa cggatggcgc tggcgcgttt ctttactggc aaaactctcc
    28981 agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa cagatcaaac
    29041 tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagtac gctatggcga
    29101 tgcattggca atagcccgcg ccgcaccgct ccggggtgac gaagaatgct caaaaacagt
    29161 tttcgcgctt ccggaatatt acacagaggt tgttgcagat ggcgacgggc gtggcgtaac
    29221 tggcgtagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat ggtgtagaac
    29281 atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag gtcgatcagc
    29341 gttccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc gggaagggcg
    29401 aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac gcggcgtgta
    29461 acgcggaagt aatccttcat catccgctcg accggttcgt taccttcacc actataattc
    29521 aaacgctggg cgacgctaag ctggcgatcg aataacaggc gattatcgta acgactgacg
    29581 accagatgca gggcaaagcg aatgcgccac aatatatgca ggcattcgtt taattccgct
    29641 ctctccgcag aggttaagaa gccaaaccct accatttcat ccagcgatgt tgctccaaaa
    29701 tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccggg gctacttttg
    29761 atgtctggtt caaggttgta gctggtgccg tggtaacgct gatggcgctg gttctgttct
    29821 tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa aatatgtttt
    29881 tgcagttcga ggaacagcgc aacatcgcca attaataagc gggattcgat taaattggtg
    29941 gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg cacgctatga
    30001 cctacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt ttgcgcctga
    30061 tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag tggatgcagc
    30121 tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg gctgaatccc
    30181 gcttcaatcc ataatcgttg aagaagctgg tcgataaact cggtacgcgc ctcaatcaac
    30241 tgttctgcgg agataccatt gtcaaaggca tcacccagcc aacgctggaa agtatcgata
    30301 tgggctttta tcccaccgac ggttaattca tcacggggcc aggcgcatgg attttgcggt
    30361 tgaccgtgca gggtggggag agcggtgttt gcgtactgtt ctggaagggt attcattgtg
    30421 cgccacccat aagattaaat tattgcatta aaaaagccgg cattcgccgg cttcatttta
    30481 ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca gaatttcgca
    30541 gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc gatctttggt
    30601 ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt tgaccattgg
    30661 ctcgatggtg aacgtcatcc ccggtttcag cacgacgttg gtttcacggg agtcatagtg
    30721 cagcacctgc ggttcttcgt ggaagccgcg accaataccg tgtccacaat attcacgaac
    30781 gacggagaag ccttctgctt cgacaaattt ctgaattgcc gcaccgattt cgcgcagatt
    30841 aatgccaggt tttaccatgc gtagcgccag gtacaggctt tcttgcgtga tgcggcacag
    30901 acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat cgccgtggaa
    30961 accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca gcagcttggc
    31021 gtcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg atttcggata
    31081 gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt aatcattaca
    31141 gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga tcatctccag
    31201 cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg gggtcttgat
    31261 tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata tatgtaagtg
    31321 ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc caaacgtgcc
    31381 actgaaggtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc gccaacaatt
    31441 attggtgtcc acgacgtatt tgtggtataa agcgcgccgg acttccgatc catttcgtat
    31501 acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg tatcggcaca
    31561 tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata accccaactt
    31621 ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca aggctggtgt
    31681 tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca tcttcggtgc
    31741 gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca acgaagctct
    31801 ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg gtactaaacg
    31861 cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct tcgtgaacca
    31921 tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca tcaaacgtct
    31981 gaaagacctg gaaactcagt ctcaggacgg tactttcgac aagctgacca agaaagaagc
    32041 gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta tcaaagacat
    32101 gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca ttgctatcaa
    32161 agaagcaaac aacctgggta ttccagtatt tgctatcgtt gataccaact ctgatccgga
    32221 cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga ccctgtacct
    32281 gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt cccaggcgga
    32341 agaaagcttc gtagaagctg agtaataagg cacgctcgta cgagccctta tagaccaggt
    32401 agtacacgtt tggttagggg gcctgcatat ggcccccttt ttcactttta tatctgtgcg
    32461 gtttaatgcc gggcagatca catctccgag gattttagaa tggctgaaat taccgcatcc
    32521 ctggtaaaag agctgcgtga gcgtactggc gcaggcatga tggattgcaa aaaagcactg
    32581 actgaagcta acggcgacat cgagctggca atcgaaaaca tgcgtaagtc cggtgctatt
    32641 aaagcagcga aaaaagcagg caacgttgct gctgacggcg tgatcaaaac caaaatcgac
    32701 ggcaactacg gcatcattct ggaagttaac tgccagactg acttcgttgc aaaagacgct
    32761 ggtttccagg cgttcgcaga caaagttctg gacgcagctg ttgctggcaa aatcactgac
    32821 gttgaagtac tgaaagcaca gttcgaagaa gaacgtgttg cgctggtagc gaaaattggt
    32881 gaaaacatca acattcgccg cgttgctgcg ctggaaggcg acgttctggg ttcttatcag
    32941 cacggtgcgc gtatcggtgt tctggttgct gctaaaggcg ctgacgaaga gctggttaaa
    33001 cacatcgcta tgcacgttgc tgcaagcaag ccagaattca tcaaaccaga agacgtatcc
    33061 gctgaagtgg tagaaaaaga ataccaggta cagctggata tcgcgatgca gtctggtaag
    33121 ccgaaagaaa tcgcagagaa aatggttgaa ggccgcatga agaaattcac cggcgaagtt
    33181 tctctgaccg gtcagccgtt cgttatggaa ccaagcaaaa ctgttggtca gctgctgaaa
    33241 gagcataacg ctgaagtgac tggcttcatc cgcttcgaag tgggtgaagg catcgagaaa
    33301 gttgagactg actttgcagc agaagttgct gcgatgtcca agcagtctta attatcgaaa
    33361 aggagccgcc tgagggcggc ttctttttgt gcccatcttg taaattcagc taacccttgt
    33421 ggggctgcgc tgaaaagcga cgtacaatgt cgctagtatt aattcatttc aatcgttgac
    33481 agtctcagga aagaaacatg gctaccaatg caaaacccgt ctataaacgc attctgctta
    33541 agttgagtgg cgaagctctg cagggcactg aaggcttcgg tattgatgca agcatactgg
    33601 atcgcatggc tcaggaaatc aaagaactgg ttgaactggg tattcaggtt ggtgtggtga
    33661 ttggtggggg taacctgttc cgtggcgctg gtctggcgaa agcgggtatg aaccgcgttg
    33721 tgggcgacca catggggatg ctggcgaccg taatgaacgg cctggcaatg cgtgatgcac
    33781 tgcaccgcgc ctatgtgaac gctcgtctga tgtccgctat tccattgaat ggcgtgtgcg
    33841 acagctacag ctgggcagaa gctatcagcc tgttgcgcaa caaccgtgtg gtgatcctct
    33901 ccgccggtac aggtaacccg ttctttacca ccgactcagc agcttgcctg cgtggtatcg
    33961 aaattgaagc cgatgtggtg ctgaaagcaa ccaaagttga cggcgtgttt actgctgatc
    34021 cggcgaaaga tccaaccgca accatgtacg agcaactgac ttacagcgaa gtgctggaaa
    34081 aagagctgaa agtcatggac ctggcggcct tcacgcttgc tcgtgaccat aaattaccga
    34141 ttcgtgtttt caacatgaac aaaccgggtg cgctgcgccg tgtggtaatg ggtgaaaaag
    34201 aagggacttt aatcacgaaa taattcccgt gatggataaa taagggtaag attccgcgta
    34261 agtatcgcgg gggatcttac ccgtaatatg tttaatcagg gctatactta gcacacttcc
    34321 actgtgtgtg actgtctggt ctgactgaga caagttttca aggattcgta acgtgattag
    34381 cgatatcaga aaagatgctg aagtacgcat ggataaatgc gtagaagcgt tcaaaaccca
    34441 aatcagcaaa atacgcacgg gtcgtgcttc tcccagcctg ctggatggca ttgtcgtgga
    34501 atattacggc acgccgacgc cgctgcgtca gctggcaagc gtaacggtag aagattcccg
    34561 tactctgaaa atcaacgtgt ttgatcgttc aatgtctccg gccgttgaaa aagcgattat
    34621 ggcgtccgat cttggcctga acccgaactc tgcgggtagc gacatccgtg ttccgctgcc
    34681 gccgctgacg gaagaacgtc gtaaagatct gaccaaaatc gttcgtggtg aagcagaaca
    34741 agcgcgtgtt gcagtacgta atgtgcgtcg tgacgcgaac gacaaagtga aagcactgtt
    34801 gaaagataaa gagatcagcg aagacgacga tcgccgttct caggacgatg tacagaaact
    34861 gactgatgct gcaatcaaga aaattgaagc ggcgctggca gacaaagaag cagaactgat
    34921 gcagttctga tttcttgaac gacaaaaacg ccgctcagta gatccttgcg gatcggctgg
    34981 cggcgttttg ctttttattc tgtctcaact ctggatgttt catgaagcaa ctcaccattc
    35041 tgggctcgac cggctcgatt ggttgcagca cgctggacgt ggtgcgccat aatcccgaac
    35101 acttccgcgt agttgcgctg gtggcaggca aaaatgtcac tcgcatggta gaacagtgcc
    35161 tggaattctc tccccgctat gccgtaatgg acgatgaagc gagtgcgaaa cttcttaaaa
    35221 cgatgctaca gcaacagggg agccgcaccg aagtcttaag tgggcaacaa gccgcttgcg
    35281 atatggctgc gcttgaggat gttgatcagg tgatggcagc cattgttggc gctgctgggc
    35341 tgttacctac gcttgctgcg atccgcgcgg gtaaaaccat tttgctggcc aataaagaat
    35401 cactggttac ctgcggacgt ctgtttatgg acgccgtaaa gcagagcaaa gcgcaattgt
    35461 taccggtcga tagcgaacat aacgctattt ttcagagttt accgcaacct atccagcata
    35521 atctgggata cgctgacctt gagcaaaatg gcgtggtgtc cattttactt accgggtctg
    35581 gtggcccttt ccgtgagacg ccattgcgcg atttggcaac aatgacgccc gatcaagcct
    35641 gccgtcatcc gaactggtcg atgggacgta aaatttccgt cgattcggct accatgatga
    35701 acaaaggtct ggaatacatt gaagcgcgtt ggctgtttaa cgccagcgcc agccagatgg
    35761 aagtgctgat tcacccgcag tcagtgattc actcaatggt gcgctaccag gacggcagtg
    35821 ttctggcgca gctgggtgaa ccggatatgc gtacgccaat tgctcacacc atggcatggc
    35881 cgaatcgcgt gaactctggc gtgaagccgc tcgatttttg caaactaagt gcgttgacat
    35941 ttgccgcacc ggattatgat cgttatccat gtctgaaact ggcgatggag gcgtttgaac
    36001 aaggccaggc agcgacgaca gcattgaatg ccgcaaatga aatcaccgtg gctgcttttc
    36061 ttgcgcaaca aatccgcttt acggatatcg ccgcgttgaa tttatccgta ctggaaaaaa
    36121 tggatatgcg cgaaccacaa tgtgtggatg atgtgttatc tgttgatgcg aacgcgcgtg
    36181 aagtcgccag aaaagaggtg atgcgtctcg caagctgagg ataatccggc tacagagagt
    36241 cgcgctattt gttagcgtag ggcttcagtg atatagtctg cgccatctga tcgtaagtag
    36301 ttggctttat aaggtcaggt atgccgtggt tttacacggc ttttttttgt ataggcttca
    36361 gtattcctga gtaccgtaaa ccctgtcagg gaataagaaa cgcgtgatgt tgtctgctac
    36421 tcaaccactt agcgaaaaat tgccagcgca tggctgtcgt catgttgcga tcattatgga
    36481 cggcaatggt cgctgggcaa aaaagcaagg gaagattcgt gcctttgggc ataaagccgg
    36541 ggcaaaatcc gtccgccggg ctgtctcttt tgcggccaac aacggtattg aggcgttaac
    36601 gctgtatgcc tttagtagtg aaaactggaa ccgaccagcg caggaagtca gtgcgttaat
    36661 ggaactgttt gtgtgggcgc tagatagcga agtaaaaagt ctgcaccgac ataacgtgcg
    36721 tctgcgtatt attggcgata ccagtcgctt taactcgcgt ttgcaagaac gtattcgtaa
    36781 atctgaagcg ctaacagccg ggaataccgg tctgacgctg aatattgcgg cgaactacgg
    36841 tggacgttgg gatatagtcc agggagtcag gcaactggct gaaaaggtgc agcaaggaaa
    36901 cctgcaacca gatcagatag atgaagagat gctaaaccag catgtctgta tgcatgaact
    36961 ggcccctgta gatttagtaa ttaggactgg gggggagcat cgcattagta actttttgct
    37021 ttggcaaatt gcctatgccg aactttactt tacagatgtt ctctggcccg atttcgatga
    37081 acaagacttt gaaggggcgt taaatgcctt tgctaatcga gagcgtcgtt tcggcggcac
    37141 cgagcccggt gatgaaacag cctgatgggg gtcgcttttg ctgaagtatc gcctgatatc
    37201 tgcttttgtg ttaatacccg tcgtcatcgc ggcgttgttt ctgttgccgc cggtggggtt
    37261 cgccattgta acgctggtgg tctgtatgct ggcagcgtgg gaatggggac agcttagcgg
    37321 ttttaccact cgttcgcagc gagtatggtt ggcggtgtta tgcgggttat tgttggcgct
    37381 gatgcttttt ctgttgccgg aatatcaccg aaatattcat caaccgctgg ttgaaatctc
    37441 actttgggct tcgctgggtt ggtggattgt cgcgctattg ctggtgctgt tttaccctgg
    37501 ttccgcagca atctggcgta actctaaaac attgcgcatt atttttggcg tgctaaccat
    37561 tgttcccttc ttctggggca tgctggcgtt acgggcctgg cactacgacg agaatcatta
    37621 cagtggcgca atatggctgc tctatgtcat gatcctggta tggggcgctg actctggcgc
    37681 atatatgttt ggcaaattgt ttggtaaaca taaactggca ccgaaggttt ctccgggtaa
    37741 aacctggcaa ggctttatcg gtggactcgc gaccgcagcg gtaatctcat ggggttatgg
    37801 catgtgggtg aatctcgacg tcgctcccgt caccttactc atttgctcta ttgtcgcagc
    37861 gttagcctca gtgctcggcg atctgaccga aagtatgttt aagcgtgaag caggaattaa
    37921 ggacagcggt catttaattc caggacacgg tggtatttta gatcgtattg atagcctgac
    37981 ggctgcggta ccggtctttg cttgcttgtt gttactggta ttcaggacgc tttaacggaa
    38041 ggtaatatgc tgagttttct ctgggatttg gcttcgttca tcgttgcact gggtgtactt
    38101 atcaccgtgc atgaatttgg tcatttctgg gttgcccggc gttgtggtgt tcgcgttgag
    38161 cgtttctcaa tagggtttgg taaggcgctc tggcggcgaa ctgataagct cggcaccgaa
    38221 tatgttatcg ccctgatccc gttgggcggt tatgtcaaaa tgctggatga gcgcgcagaa
    38281 ccggtcgttc cggaactccg ccaccatgcc ttcaataata aatctgtcgg ccaacgagcg
    38341 gcgattattg ccgcaggtcc ggttgcaaac ttcatttttg ctatctttgc ctactggctg
    38401 gtttttatta ttggtgtgcc tggcgtacgt ccggtggttg gtgaaatagc agccaattcg
    38461 atagctgcgg aagcacaaat tgcaccaggt acggaactaa aagccgtaga tggtatcgaa
    38521 acgcctgatt gggatgccgt gcgtttgcag ttggtcgata aaattggcga tgaaagcacc
    38581 accattacgg tagcgccatt tggcagcgac caacggcggg atgtaaagct cgatttacgt
    38641 cactgggcgt ttgagcctga taaagaagat ccggtatctt cgttggggat tcgtcctcgt
    38701 gggccgcaaa ttgaacctgt actggaaaat gtgcagccaa actcggcggc aagcaaggca
    38761 ggtttgcaag caggcgacag gatcgttaaa gtcgatggtc agcccttaac gcagtgggtg
    38821 acctttgtga tgcttgtccg ggataacccg ggtaaatcct tagcgttaga aatcgaaagg
    38881 caggggagtc ccttgtcttt gacattaatc ccggagagta aaccgggtaa gggtaaagcg
    38941 attggttttg tcggtattga gccgaaagtc attcctttgc cagatgagta taaagttgta
    39001 cgccagtatg ggccgttcaa cgctatcgtc gaagccacgg acaaaacgtg gcagttgatg
    39061 aagctaacgg tcagtatgct gggaaaattg atcaccggtg atgtgaaact gaacaacctc
    39121 agtgggccga tctctatcgc caagggggct gggatgacag cggaactcgg ggtagtttat
    39181 tacctgccgt ttcttgcgct tattagcgtg aacttaggga taattaacct gtttccgttg
    39241 cccgtacttg acggggggca tctgctgttc cttgcgatcg aaaagatcaa gggcggaccg
    39301 gtatccgagc gggttcaaga cttttgttat cgcattggct cgattctgct ggtgctgtta
    39361 atggggcttg cacttttcaa tgatttctct cggttatgag agttagttag gaagaacgca
    39421 taataacgat ggcgatgaaa aagttgctca tagcgtcgct gctgtttagc agcgccaccg
    39481 tatacggtgc tgaagggttc gtagtgaaag atattcattt cgaaggcctt cagcgtgtcg
    39541 ccgttggtgc ggccctcctc agtatgccgg tgcgcacagg cgacacggtt aatgatgaag
    39601 atatcagtaa taccattcgc gctctgtttg ctaccggcaa ctttgaggat gttcgcgtcc
    39661 ttcgtgatgg tgataccctt ctggttcagg taaaagaacg tccgaccatt gccagcatta
    39721 ctttctccgg taacaaatcg gtgaaagatg acatgctgaa gcaaaacctc gaggcttctg
    39781 gtgtgcgtgt gggcgaatcc ctcgatcgca ccaccattgc cgatatcgag aaaggtctgg
    39841 aagacttcta ctacagcgtc ggtaaatata gcgccagcgt taaagctgtc gtgaccccgc
    39901 tgccgcgcaa ccgtgttgac ctaaaactgg tgttccagga aggtgtgtca gctgaaatcc
    39961 agcaaattaa cattgttggt aaccatgctt tcaccaccga tgaactgatc tctcatttcc
    40021 aactgcgtga cgaagtgccg tggtggaacg tggtaggcga tcgtaaatac cagaaacaga
    40081 aactggcagg cgaccttgaa accctgcgca gttactatct ggatcgcggt tatgcccgtt
    40141 tcaacatcga ctctacccag gtcagtctga cgccagataa aaaaggtatt tacgtcacgg
    40201 tgaacatcac cgaaggcgat cagtacaagc tttctggcgt tgaagtgagc ggcaaccttg
    40261 ccgggcactc cgctgaaatt gagcagctga ctaagatcga gccgggcgag ctgtataacg
    40321 gcaccaaagt gaccaaaatg gaagatgaca ttaaaaagct tcttggtcgc tatggttatg
    40381 cctatccgcg cgtacagtcg atgcctgaaa ttaacgatgc cgacaaaacc gttaaattac
    40441 gcgtgaacgt tgatgcgggt aaccgtttct acgtgcgtaa gatccgtttt gaaggtaacg
    40501 atacctcgaa agatgccgtc ctgcgtcgcg aaatgcgtca gatggaaggt gcatggctgg
    40561 ggagcgatct ggtcgatcag ggtaaggagc gtctgaatcg tctgggcttc tttgaaactg
    40621 tcgataccga tacccaacgt gttccgggta gcccggacca ggttgatgta gtctacaagg
    40681 tgaaagaacg taacaccggt agcttcaact tcgggattgg ttacggtact gaaagtggcg
    40741 tgagcttcca ggctggtgtg caacaggata actggttagg tacgggttat gctgttggta
    40801 tcaacgggac caaaaacgat taccagacct atgctgaact gtcggtaacc aacccgtact
    40861 tcaccgtaga tggcgtaagc ctcggtggtc gtctcttcta taatgacttc caggcagatg
    40921 acgccgacct gtccgactat accaacaaga gttatggtac agatgtgacg ttgggcttcc
    40981 cgatcaacga atataactcg ctgcgtgcag gtctgggtta tgtacataac tccctgtcca
    41041 acatgcagcc tcaggttgcg atgtggcgct atctgtactc tatgggtgaa catccgagca
    41101 cctctgatca ggataacagc ttcaaaacgg acgacttcac gttcaactat ggttggacct
    41161 acaacaagct tgaccgtggt tacttcccga cagatggttc acgtgtcaac ctgaccggta
    41221 aagtgaccat tccgggatcg gataacgaat actacaaagt gacgttagac acggcgactt
    41281 atgtgccgat tgatgatgat cacaaatggg ttgttctggg tcgtacccgc tggggttatg
    41341 gtgatggttt aggcggcaaa gagatgccgt tctatgagaa cttctatgcc ggtggttcca
    41401 gcaccgtgcg tggcttccag tccaatacca ttggtccgaa agcagtttac ttcccgcatc
    41461 aggccagtaa ttatgatccg gactatgatt acgaatgtgc gactcaggac ggcgcgaaag
    41521 atctgtgtaa atcggatgat gctgtaggcg gtaacgccat ggcggttgcc agcctcgagt
    41581 tcatcacccc gacgccgttt attagcgata agtatgctaa ctcggttcgt acttccttct
    41641 tctgggatat gggtaccgtt tgggatacaa actgggattc cagccaatat tctggatatc
    41701 cggactatag tgatccaagc aatatccgta tgtctgcggg tatcgcatta caatggatgt
    41761 ccccattggg gccgttggtg ttctcctacg cccagccgtt caaaaagtac gatggagaca
    41821 aggcagaaca gttccagttt aacatcggta aaacctggta agtgttctcc acaaaggaat
    41881 gtagtggtag tgtagcgatg actttaggcg atcaatataa gatcgccggg ccacgcaaag
    41941 aactgcaccc tccggtgcaa atgggatggt aaggagttta ttgtgaaaaa gtggttatta
    42001 gctgcaggtc tcggtttagc actggcaact tctgctcagg cggctgacaa aattgcaatc
    42061 gtcaatatgg gcagcctgtt ccagcaggta gcgcagaaaa ccggtgtttc taacacgctg
    42121 gaaaacgagt tcaaaggccg tgccagcgaa ctgcagcgta tggaaaccga tctgcaggct
    42181 aaaatgaaaa agctgcagtc catgaaagcg ggcagcgatc gcactaagct ggaaaaagac
    42241 gtgatggctc agcgccagac ttttgctcag aaagcgcagg cttttgagca ggatcgcgca
    42301 cgtcgttcca acgaagaacg cggcaaactg gttactcgta tccagactgc tgtgaaatcc
    42361 gttgccaaca gccaggatat cgatctggtt gttgatgcaa atgccgttgc ttacaacagc
    42421 agcgatgtaa aagacatcac tgccgacgta ctgaaacagg ttaaataagt aatgccttca
    42481 attcgactgg ctgatttagc gcagcagttg gatgcagaac tacacggtga tggcgatatc
    42541 gtcatcaccg gcgttgcgtc catgcaatct gcacaaacag gtcacattac gttcatggtt
    42601 aaccccaaat accgtgagca tttaggcttg tgccaggcgt ccgcggttgt catgacccag
    42661 gacgatcttc ctttcgcgaa aagtgccgcg ctggtagtga agaatcccta cctgacttac
    42721 gcgcgtatgg cgcaaatttt agataccacg ccgcagcccg cgcagaacat tgcacccagt
    42781 gcggtgattg acgcgacggc gaagctgggt aacaacgtat cgattggcgc taacgcggtg
    42841 attgagtccg gcgttgaact gggcgataac gtgattatcg gtgccggttg cttcgtaggt
    42901 aaaaacagca aaatcggtgc aggttcgcgt ctctgggcga acgtaaccat ttaccatgag
    42961 atccagatcg gtcagaattg cctgatccag tccggaacag tggtaggcgc agacggcttt
    43021 ggttatgcca acgatcgtgg taactgggtg aagatcccac agattggtcg cgtaattatt
    43081 ggcgatcgcg tggagatcgg tgcctgcact accattgatc gtggcgcgct ggatgacact
    43141 gttattggca atggtgtgat cattgataac cagtgccaga ttgcacataa cgtcgtgatt
    43201 ggcgacaata cggcagttgc cggtggcgtc attatggcgg gcagcctgaa aattggtcgt
    43261 tactgcatga tcggcggagc cagcgtaatc aacgggcata tggaaatatg cgacaaagtg
    43321 acggttacgg gcatgggtat ggtgatgcgt cccatcactg aaccaggcgt ctattcctca
    43381 ggcattccgc tgcaacccaa caaagtctgg cgcaaaaccg ctgcactggt gatgaacatt
    43441 gatgacatga gcaagcgtct gaaatcgctt gagcgcaagg ttaatcaaca agactaacgt
    43501 tccatctttt gttcgccaaa ctttacggcc tgtctcattc ttacgattgc ggcaggccgt
    43561 gttattattg tcgtttctta tattttgaca ggaagagtat cttgactact aacactcata
    43621 ctctgcagat tgaagagatt ttagaacttc tgccgcaccg tttcccgttc ttactggtgg
    43681 atcgcgtgct ggattttgaa gaaggtcgtt ttctgcgcgc agtaaaaaat gtctctgtca
    43741 atgagccatt cttccagggc catttccctg gaaaaccgat tttcccgggt gtgctgattc
    43801 tggaagcaat ggcacaggca acaggtattc tggcgtttaa aagcgtagga aaactggaac
    43861 cgggtgagct gtactacttc gccggtattg acgaagcgcg ctttaagcgc ccggtcgtgc
    43921 ctggcgatca aatgatcatg gaagtcactt tcgaaaaaac gcgccgcggc ctgacccgtt
    43981 ttaaaggggt tgctctggtc gacggtaaag tagtttgcga agcaacgatg atgtgtgctc
    44041 gtagccggga ggcctgatac gtgattgata aatccgcctt tgtgcatcca accgccattg
    44101 tggaagaggg cgcgtcgatt ggcgcgaacg ctcacattgg tcctttttgt atcgttggac
    44161 cccatgtcga aattggtgag ggtaccgtac tgaaatctca cgttgtcgtg aatggtcata
    44221 ctaaaattgg ccgcgataat gagatttatc agttcgcctc catcggcgaa gttaaccagg
    44281 atctgaaata tgctggcgaa ccgacccgcg tggaaatcgg cgatcgtaac cgcattcgcg
    44341 aaagcgtcac cattcatcgt ggcacagtcc agggcggtgg attgacgaag gtgggcagcg
    44401 acaacttact gatgatcaat gcgcacattg cgcacgattg tacggtaggt aaccgctgca
    44461 tcctcgccaa caacgcaacg ctggcgggtc acgtatcggt tgacgacttc gcgatcatcg
    44521 gcggcatgac cgcagtccat cagttctgca tcattggtgc gcatgtgatg gttggcggct
    44581 gctccggtgt ggcgcaggac gtccctcctt atgtcattgc gcagggtaac cacgcaacgc
    44641 cgttcggtgt caatatcgaa gggctgaagc gccgcggatt cagccgtgag gcgattaccg
    44701 ctatccgcaa tgcgtataag ctgatttatc gtagcggtaa aacgctcgat gaagtgaaac
    44761 cggaaattgc tgaactggcg gaaacatatc cggaagtgaa agcctttacc gatttctttg
    44821 cacgctcaac gcgcggtctg attcgttaat gactgaacag cgtccattaa cgattgccct
    44881 ggtcgccgga gaaacctccg gcgatatcct gggggccggt ttaatccgcg ctctgaaaga
    44941 acgtgtgccc aacgcccgct ttgttggtgt tgccgggcca cgaatgcagg ctgaaggctg
    45001 cgaagcctgg tacgaaatgg aagaactggc agtgatgggc attgttgaag tgctcggtcg
    45061 tctgcgtcgc ttactacata ttcgtgccga tctgacaaag cgttttggcg aactgaagcc
    45121 agatgttttt gttggtattg atgcgcctga cttcaatatt actcttgaag gtaacctcaa
    45181 aaagcagggt atcaaaacca ttcattacgt cagtccgtcc gtctgggcgt ggcgacagaa
    45241 acgcgttttc aaaataggca gagccaccga tctggtgctc gcatttctgc ctttcgaaaa
    45301 agcgttttat gacaaataca acgtaccgtg ccgctttatc ggtcatacca tggctgatgc
    45361 catgccatta gatccagata aaaatggtgc ccgtgatgtg ctggggatcc cttacgatgc
    45421 ccactgtctg gcattgttgc cgggcagccg tggtgcagaa gtcgaaatgc ttagtgccga
    45481 tttcctgaaa acggcccagc ttttgcgcca gacatatccg gatctcgaaa tcgtggtgcc
    45541 gctggtgaat gccaaacgcc gcgagcagtt tgaacgcatc aaagctgcag tcgcgccaga
    45601 cctgtcggtt catttgctgg atggaatggg ccgtgaggcg atggtcgcca gtgatgcggc
    45661 gctactggcg tcgggtacgg cagcactgga gtgtatgctg gctaaatgcc cgatggtagt
    45721 gggatatcgc atgaagcctt tcaccttctg gctggcgaag cgactggtga aaactgatta
    45781 tgtctcgctg ccaaatctgc tggcgggcag agagttagtc aaagagttat tgcaggaaga
    45841 gtgtgagccg caaaaattgg ctgcggcgct gttaccgctg ttggcgaacg ggaaaaccag
    45901 ccatgcgatg cacgacacct tccgcgagct gcatcagcag atccgctgca atgccgatga
    45961 gcaggcggca caagccgttc tggagttagc acaatgatcg aatttgtcta tccgcacacg
    46021 cagctggttg cgggtgtgga tgaagtcgga cgcgggccgt tagttggcgc ggtcgtcacc
    46081 gctgcggtga tccttgaccc ggcgcgccca attgccgggc tgaatgattc caaaaagctg
    46141 agcgaaaaac gccgtctggc gctctgtgaa gagatcaaag agaaagcgtt gagctggagt
    46201 ctgggccgtg cggaacctca cgaaatcgac gaactgaata ttttacatgc gactatgctg
    46261 gcgatgcagc gtgccgtcgc tggactgcat attgcgccgg aatatgtgtt gattgatggt
    46321 aaccgctgcc cgaaattacc gatgccttcg atggctgtgg tgaaaggcga tagccgcgta
    46381 ccggaaatca gtgccgcgtc tatcctggcg aaagtgacgc gtgacgccga aatggcggcg
    46441 ctggatattg ttttcccgca atatggtttt gcccaacaca aagggtatcc aaccgctttt
    46501 catctggaaa aactggctga acacggcgcg accgaacacc atcggcgcag ctttgggcct
    46561 gtcaaacgcg cactgggact tgcgtcctga ttcttgtgtc gagattaagt aaaccggaat
    46621 ctgaagatgt ctgaaccacg tttcgtacac ctgcgggtgc acagcgacta ctcgatgatc
    46681 gatggcctgg ccaaaaccgc gccgctggta aaaaaggcgg cggcgttggg tatgccagca
    46741 ctggcgatca ccgatttcac caacctttgc ggtctggtga agttctacgg agcgggacat
    46801 ggcgcaggga ttaagcccat cgtcggggcg gattttaacg tccagtgcga cctgctgggt
    46861 gatgagttaa ctcacctgac ggtgctggcg gcgaacaata ccggctatca gaatctgacg
    46921 ttgctgatct caaaagcgta tcagcgcggg tacggtaccg ccgggccgat catcgatcgc
    46981 gactggctta tcgaattaaa cgaagggctg atcctccttt ccggcgggcg catgggcgac
    47041 gtcgggcgca gccttttgcg tggtaacagc gcgctggtag atgagtgcgt tgcgttttat
    47101 gaagagcact tcccggatcg ctattttctc gagctgatcc gcaccggcag gccggacgaa
    47161 gaaagttatc tgcacgcggc agtggaactg gcggaatcac gcggtttgcc cgttgtggcg
    47221 accaacgacg tgcgctttat cgacagcagc gactttgacg cacacgaaat ccgcgtcgcg
    47281 atccacgacg gctttaccct cgacgatcct aaacgcccgc gtaactattc gccgcagcaa
    47341 tatatgcgta gcgaagagga gatgtgcgag ctttttgccg acattcccga agcccttgcc
    47401 aacaccgttg agatcgccaa acgttgtaac gtaactgtgc gtctgggtga atacttcttg
    47461 ccgcaattcc cgaccgggga catgagtacc gaagattatc tggtcaagcg tgcaaaagaa
    47521 ggcctggaag agcgtctggc ctttttattc cctgacgagg aagaacgtct taagcgtcgt
    47581 ccggagtatg acgaacgtct ggatactgaa cttcaggtta tcaaccagat gggcttcccg
    47641 ggttacttcc tcatcgttat ggaatttatc cagtggtcga aagataacgg tgtaccggta
    47701 gggcctggcc gtggttctgg tgcagggtca ctggtggcct acgcgttaaa aatcaccgat
    47761 ctcgatccgc tggaatttga cctgctgttc gaacgtttcc ttaacccgga acgtgtctcc
    47821 atgcctgact tcgacgttga cttctgtatg gagaaacgtg accaggttat cgaacatgtg
    47881 gcggacatgt acggtcgcga tgcggtatcg cagattatca ccttcggtac gatggcggcg
    47941 aaagcggtga tccgcgacgt aggccgtgta ttggggcatc cgtacggatt tgtcgatcgt
    48001 atctcgaaac tgatcccgcc cgatccgggt atgacgctgg cgaaagcgtt tgaagccgag
    48061 ccgcagctgc cggaaatcta cgaagcggat gaagaagtta aggcgctgat cgacatggcg
    48121 cgcaaactgg aaggggtcac ccgtaacgcc ggtaagcacg ccggtggggt ggttatcgcg
    48181 ccgaccaaaa ttactgattt tgcgccgctt tattgcgatg aagagggcaa acatccggtc
    48241 acccagtttg ataaaagcga cgttgaatac gccgggctgg tgaagttcga cttcctcggt
    48301 ttgcgtacgc tcaccatcat caactgggcg ctggagatga tcaacaagcg gcgggcgaag
    48361 aatggcgagc cgccgctgga tatcgccgcg atcccgctgg atgacaagaa aagcttcgac
    48421 atgctgcaac gctcggaaac cacggcggta ttccagcttg aatcgcgcgg catgaaggac
    48481 ctgatcaagc gtctgcaacc tgactgcttc gaagatatga tcgcactggt ggcactgttc
    48541 cgccccggcc cgttgcaatc agggatggtg gataacttta tcgaccgtaa acatggtcgc
    48601 gaagagatct cctatccgga cgtgcagtgg cagcatgaaa gcctgaaacc ggtactggag
    48661 ccaacctacg gcatcatcct gtatcaggaa caggtcatgc agattgccca ggtgctttct
    48721 ggttataccc tcggtggcgc ggatatgctg cgtcgtgcga tgggtaagaa aaagccggaa
    48781 gagatggcta agcagcgttc tgtatttgct gaaggtgcag aaaagaacgg aatcaacgcc
    48841 gaactggcga tgaaaatctt cgacctggtg gagaaattcg cgggttacgg atttaacaaa
    48901 tcgcactcgg cggcctatgc tttggtgtca tatcaaacgt tatggctgaa agcgcactat
    48961 ccggcggagt ttatggcggc ggtaatgacc gccgatatgg acaacaccga gaaggtggtg
    49021 ggcctggtgg atgagtgctg gcggatgggg ctgaaaatcc tgccaccaga tataaactcc
    49081 ggtctttacc atttccacgt caacgacgac ggcgaaatcg tgtatggtat tggcgcgatc
    49141 aaaggggtag gtgaaggccc gattgaggcc atcatcgaag cccgtaataa aggcggctac
    49201 ttccgcgaac tgtttgatct ctgcgcccgt accgacacca aaaagttaaa ccggcgggtg
    49261 ctggaaaaac tgatcatgtc cggggcgttt gaccgtcttg ggccacaccg cgcggcgctg
    49321 atgaactcgc tgggcgatgc gttaaaagcg gcagatcaac acgcgaaagc ggaagctatc
    49381 ggtcaggccg atatgttcgg cgtgctggcc gaagagccgg aacaaattga acaatcctac
    49441 gccagctgcc aaccgtggcc ggagcaggtg gtattagatg gggaacgtga aacgttaggt
    49501 ctgtacctga cgggacaccc tatcaaccag tatttaaaag agattgagcg ttatgtcgga
    49561 ggcgtaaggc tgaaagacat gcacccgaca gaacgtggta aagtcatcac ggctgcgggg
    49621 ctcgttgttg ccgcgcgggt tatggtcacc aagcgcggca atcgtatcgg tatctgcacg
    49681 ctggatgacc gttccgggcg gctggaagtg atgttgttta ctgacgccct ggataaatac
    49741 cagcaattgc tggaaaaaga ccgcatactt atcgtcagcg gacaggtcag ctttgatgac
    49801 ttcagcggtg ggcttaaaat gaccgctcgc gaagtgatgg atattgacga agcccgggaa
    49861 aaatatgctc gcgggcttgc tatctcgctg acggacaggc aaattgatga ccagctttta
    49921 aaccgactcc gtcagtctct ggaaccccac cgctctggga caattccagt acatctctac
    49981 tatcagaggg cggatgcacg cgcgcgattg cgttttggcg cgacgtggcg tgtctctccg
    50041 agcgatcgtt tattaaacga tctccgtggc ctcattggtt cggagcaggt ggaactggag
    50101 tttgactaat acaggaatac tatgagtctg aatttccttg attttgaaca gccgattgca
    50161 gagctggaag cgaaaatcga ttctctgacc gcggttagcc gtcaggatga gaaactggat
    50221 attaacatcg atgaagaagt gcatcgtctg cgtgaaaaaa gcgtagaact gacgcgtaaa
    50281 atcttcgccg atctcggtgc atggcagatt gcgcaactgg cacgccatcc acagcgtcct
    50341 tataccctgg attacgttcg cctggcattt gatgaatttg acgaactggc tggcgaccgc
    50401 gcgtatgcag acgataaagc tatcgtcggt ggtatcgccc gtctcgatgg tcgtccggtg
    50461 atgatcattg gtcatcaaaa aggtcgtgaa accaaagaaa aaattcgccg taactttggt
    50521 atgccagcgc cagaaggtta ccgcaaagca ctgcgtctga tgcaaatggc tgaacgtttt
    50581 aagatgccta tcatcacctt tatcgacacc ccgggggctt atccgggcgt gggcgcagaa
    50641 gaacgtggtc agtcggaagc cattgcacgc aacctgcgtg aaatgtctcg cctcggcgta
    50701 ccggtagttt gtacggttat cggtgaaggt ggttctggtg gtgcgctggc gattggcgtg
    50761 ggcgataaag tgaatatgct gcaatacagc acctattccg ttatctcgcc ggaaggttgt
    50821 gcgtccattc tgtggaagag cgctgacaaa gcgccgctgg cggctgaagc gatgggtatc
    50881 atcgctccac gtctgaaaga gctgaaactg atcgactcca tcatcccgga accgctgggc
    50941 ggtgctcacc gtaacccgga agcgatggcg gcgtctctga aagcgcagct gctggcggat
    51001 ctggccgatc tcgacgtgtt aagcactgaa gatttaaaaa atcgtcgtta tcagcgcctg
    51061 atgagctacg gctacgcgta atcggcaaat gttctgaaaa gggtcacttc ggtggccctt
    51121 ttttatcgcc acggtttgag caggctatga ttaaggaagg attttccagg aggaacacat
    51181 gaacatcatt gccattatgg gaccgcatgg cgtcttttat aaagatgagc ccatcaaaga
    51241 actggagtcg gcgctggtgg cgcaaggctt tcagattatc tggccacaaa acagcgttga
    51301 tttgctgaag tttatcgaac ataacccacg aatttgcggc gtgatttttg actgggatga
    51361 gtacagtctc gatttatgta gcgatatcaa tcaacttaat gaatatctcc cgctttatgc
    51421 cttcatcaac acccactcga cgatggatgt cagcgtgcag gatatgcgga tggcgctctg
    51481 gttttttgaa tatgcgctgg ggcaggcgga agatatcgcc attcgtatgc gtcagtatac
    51541 caacgaatat cttgataaca ttacgccgcc gttcacgaaa gccttgttta cctacgtcaa
    51601 agagcggaag tacacctttt gtacgccggg gcatatgggc ggcaccgcat atcaaaaaag
    51661 cccggttggc tgtctgtttt atgatttttt cggcgggaat accctcaagg cggacgtctc
    51721 tatttcggtc accgagcttg gttcgttgct cgaccacacc gggccacacc tggaagcgga
    51781 agagtacatc gcgcggacgt ttggcgcgga acagagttat atcgttacca acggaacatc
    51841 gacgtcgaac aaaattgtgg gtatgtacgc cgcgccatcc ggcagtacgc tgttgatcga
    51901 ccgcaattgt cataaatcgc tggcgcatct gttgatgatg aacgatgtag tgccagtctg
    51961 gctgaaaccg acgcgtaatg cgttggggat tctgggtggg atcccgcgcg gtgaatttac
    52021 tcgcgacagc atcgaagaga aagtcgctgc caccacgcag gcacaatggc cggttcatgc
    52081 ggtgatcacc aactccacct atgatggctt gctctacaac accgactgga tcaaacagac
    52141 gctggatgtc ccgtcgattc acttcgattc tgcctgggtg ccgtacaccc attttcatcc
    52201 aatctaccag ggtaaaagtg gtatgagcgg cgagcgtgtt gcaggaaaag tgatcttcga
    52261 aacgcaatct acccacaaaa tgctggcggc gttatcgcag gcttcgctga tccacattaa
    52321 aggcgagtat gacgaggagg cgttcaacga agcctttatg atgcatacca ccacctcgcc
    52381 cagttatccc attgttgctt ctgttgagac ggcggcggcg atgttgcgtg gtaatccggg
    52441 caaacggctg attaaccgtt cagtagaacg agctctgcat tttcgcaaag aggtccagcg
    52501 gctgcgggaa gagtctgacg gctggttttt cgatatctgg caaccgccgc aggtggatga
    52561 agccgaatgc tggcccgttg cgcctggcga acagtggcac ggctttagcg atgcggatgc
    52621 caatcacatg tttctcgatc cggttaaagt cactattttg acaccgggga tggacgagca
    52681 gggcaatatg agcgaggagg ggatcccggc ggcgctggtg gcaaaattcc tcgacgaacg
    52741 tgggatcgta gtagagaaaa ccggccctta taacctgctg tttctcttta gtattggcat
    52801 cgataaaacc aaagcaatgg gattattgcg tgggttgacg gaatttaagc gctcttacga
    52861 tctcaacctg cggatcaaaa atatgctgcc cgatctctat gcagaagatc ccgatttcta
    52921 ccgcaatatg cgtattcagg atctggcgca agggatccat aagctgattc gtaaacacga
    52981 tcttcccggt ttgatgttgc gggcatttga taccttgccg gagatgatca tgacgccaca
    53041 tcaggcatgg cagagacaga ttaaaggcga agtagaaacc attgcgctgg aacaactggt
    53101 cggtagagta tcggcaaata tgatcctgcc ttatccaccc ggcgtaccgc tgctgatgcc
    53161 gggagaaatg cttaccgaag agagccgcac ggtactcgat tttctactga tgctttgttc
    53221 cgtagggcaa cattaccccg gttttgaaac ggatattcac ggcgcgaaac atgacgaaga
    53281 cggtgtttac cgcgtacgag tcctaaaaat ggcaggataa cttgccagag cggcttccgg
    53341 gcgagtaacg tgctgttaac aaataaagga gacgttatgc tgggtttaaa acaggttcac
    53401 catattgcga ttattgcgac ggattatgcg atgagcaaag ctttctactg cgatattctt
    53461 ggtttcacgc tgcaaagcga agtctatcgc gaagcgcgcg actcgtggaa aggggatttg
    53521 gcgcttaatg ggcaatatgt gattgagctt ttctcatttc cgttcccgcc ggagcgcccc
    53581 agccgaccgg aagcttgcgg cctgcgtcat ctggctttta gcgttgatga tatcgatgcg
    53641 gcagtggcgc accttgaaag ccataacgtg aagtgtgaag ccatccgtgt cgatccctac
    53701 acgcaaaaac gcttcacgtt ctttaacgat ccggacgggc tgccgttgga actgtatgag
    53761 cagtaaggct tgtcatcgcc gcatttgccc ggtaacgtgc ccggcattgc tactgtaaaa
    53821 tcgcaccatc atgacactca cgctcaatag acaacttctc tcctcacgcc agattctggt
    53881 ggcctttagc ggcggccttg attctactgt cttactgcat caactggtgc agtggcggac
    53941 ggaaaatccg ggcgtcactc tgcgcgctat ccatgtgcat cacggtttaa gcgccaatgc
    54001 tgatgcctgg gtgaaacatt gtgaaaacat ctgccaacag tggcaggtgc cgctggtggt
    54061 cgaacgtgtg cagcttgcgc aagaggggct aggtattgag gcccaggcgc ggcaggcacg
    54121 ttatcaggca tttgcccaca ccttgttgcc cggtgaagtg ctggttacag cgcaacatct
    54181 cgacgatcaa tgtgaaacct ttctgctggc gctaaaacgc ggtagcggcc ctgccgggct
    54241 ttcggctatg gcggaagttt cggagtttgc cggaacacag cttatccgcc cgttgcttgc
    54301 ccgcacgcgg ggggaactgg tgcagtgggc gttggcgcat ggtttacgct ggattgaaga
    54361 cgaaagtaat caggatgata gctacgatcg taactttctg cgcctgcgcg tagtgccgtt
    54421 attgcagcag cgttggccgc attttgccga agcaacagcc cgcagcgccg cactttgcgc
    54481 tgaacaagag agcctgttag atgaactgct ggcggatgat ttagcgcact gtcaaactcc
    54541 gcaggggacg ctgcagattg cgctaatgct ggcgatgagt gatgcccgtc gcgcggcgat
    54601 tatccgccgc tggctggcag ggcagaatgc accgatgcct tcccgcgacg cgttggtgag
    54661 gatctggcag gaagtggcgc tggcgcggga agatgcctcc ccctgtttac gtttgggcgc
    54721 gtttgaaatc cgccgctatc agtcgcaact atggtggatt aaatccgttg ccgggcaaag
    54781 cgaaaccatt gtgccgtggc agacgtggct acaaccgctg gaattaccgg cggggctggg
    54841 aagtgtacag cttactgcgg gaggcgatat tcgccctccg cgtgcagacg aagcggtcag
    54901 cgtgcgtttc aaagcgccag gattgctgca tattgtcggg cgcaacggcg gacgtaagct
    54961 gaagaaaatc tggcaagagt taggcgttcc gccgtggcta cgtgacacca cgccactgct
    55021 gttttatggc gaaacgctga ttgcggcggc aggggtattt gtgacgcaag aaggtgtggc
    55081 tgtaggtgag aatggcgtca gttttgtctg gcagaaaacg cttagtcaag tgaaagccgg
    55141 ataagacgca tcaaacgtcg catccggcga aagtcaatca ggactcgctc accaccaccg
    55201 taccgatttc cgggtggcta aagctggtaa ttttatccag acgcagctcg cgggttgcac
    55261 cagcggcctc gacgaccaga tactccacat ttttgcggga gactaaatcg ctggccttcg
    55321 cctgcaattt ttcgccatct ttcagctcaa gtgtcagcat taaatgatgc tggcaggcga
    55381 gctcaagatt atcgtaatca tcacaattga ttggttgata cgtatcattc attgacataa
    55441 tcgctcacca gtaagtttgc cgcagcgtat gctgcttttt ccctgacagc ctcagaaagg
    55501 gcgtcgtcgg cagccatttc attcagcact ttcaaaacgc agcccagcgc gtccggaacg
    55561 tatcctaagt ctccgctggc gatttccgcg taccgcttgc gtattaactc acaatatttt
    55621 tccacatgcc ctcctgtcag cactctgact taaccgtgga tgcaagtcta agcctacgaa
    55681 gataaactct gtttcgcaag gtgactatac cacactcatt tctgcaatat cagcgccgca
    55741 actgcacgta ttccgttaca atggcctcct gattcgaaag gagttttctt atggcgctta
    55801 aagcgacaat ttataaagcg acggttaatg tggccgatct cgaccgcaac cagtttctcg
    55861 atgcctctct gacgctggcg cgccatcctt cagaaaccca ggagcgcatg atgctgcgcc
    55921 tgctggcgtg gctaaaatat gccgatgaac gtctgcaatt tacccgtggt ttgtgtgccg
    55981 atgatgagcc ggaagcgtgg ctgcgtaacg atcacctggg cattgatttg tggattgaac
    56041 tggggctgcc ggatgagcgg cggattaaga aagcctgcac ccaggccgca gaagtggcgc
    56101 tgtttgccta taatagtcgg gcggcgcaaa tctggtggca gcaaaatcag agcaaatgtg
    56161 cgcagtttgc caatctttcc gtctggtatc tggacgacga gcagctggcg aaagtgagcg
    56221 cctttgccga tcgtaccatg acgctgcagg caacgattca agatggtgtt atctggttat
    56281 cggatgataa gaataatctg gaagtgaact taaccgtctg gcaacaacct tcatgattgt
    56341 gatttcccga catgttgcta tctctgatgg cgagtttgag atcaccgcca ttcgtgcgca
    56401 gggcgcgggc gggcagcatg ttaataagac ctcaacggct attcatctgc gttttgacat
    56461 tcgggcgtcc agcctgccag agtattacaa agaacgtctg ctcgccgcca gccatcactt
    56521 gatcagcagt gatggggtga ttgtcattaa ggcacaggaa taccgcaatc aggagctcaa
    56581 ccgcgaagcg gcgctggccc ggctggtggc agtgattaaa gatttaacaa cagaacaaaa
    56641 agcccgacga cccacgcggc ccacccgcgc atcaaaagag cgcaggctgg catcgaaagc
    56701 acaaaaatca agcgtgaagg cgatgcgcgg caaagtgcgc agcggtcggg aataaaaaga
    56761 aggaatggat ggtgaaaaaa gcgatagtga cagcgatggc tgtaatcagc ctctttactc
    56821 tgatgggatg taataatcgg gccgaagtcg atacgctttc tccggcacag gctgccgaac
    56881 tgaaaccgat gccgcaaagt tggcgtggcg tgctgccgtg tgctgattgc gaaggaatcg
    56941 aaacctctct gttcctcgaa aaagacggaa cctgggtgat gaatgagcgt tatctggggg
    57001 ctcgtgaaga accttcctcc ttcgcttcct acggtacatg ggcgcgaacc gctgacaagc
    57061 tggtattaac cgatagcaaa ggtgaaaagt catattatcg cgcgaaagga gatgcgctgg
    57121 agatgctcga tcgtgaaggt aatccgattg aatcgcagtt caactacacg ctggaaccgg
    57181 cacaatccag cttacccatg acgccgatga ccctgcgggg catgtatttt tatatggctg
    57241 atgcggcgac cttcactgat tgcgcgaccg gaaaacgttt catggtggcg aataacgcag
    57301 agctggagcg tggctacctg gctgcgcgca gtaacagtga aaaaccggtg ttactgtcag
    57361 tagaaggtca ctttacgctt gaggctaatc cggataccgg tgcgccgacc aaagtattag
    57421 cgcccgatac ggcaggtaaa ttttacccaa accaggattg cagtagtttg gggctgtaac
    57481 ccgtcttgag acagaaacaa atgaaatatt gcctcatgcg ctgcgcttat cagtcctaaa
    57541 gcatacaatg caatattttg aattagcaca attttgtagg ccggataagg cgttcacgcc
    57601 gcatccggca ttttgcgcac gaggctggct tttatcactg agtcagccca acaaagcggc
    57661 ctgccttgat ataaatccca ggcttcatgt gcccaacata ctgtaactgc gtttctggct
    57721 taaacgcaga gatatcgcca gtacgcatat gcatcagatc ggcagggcta atccagcctg
    57781 attgcgccag cgtaagcgga tgcccggctt tggcaaatgc atcagtgaca aattccgaac
    57841 aaaaccacga ctttttgtct ccttcgccca cactgcttaa ctgcggtttc gccaggccgc
    57901 tgacgcactg ttgacgaaaa tcctcggaga acggatttag tgagcacatc tggcgagtca
    57961 ccataaatgg aataaattcg acaatgccgc gatagttgta accgctatct ttaattttgt
    58021 tggcgaacgc ggtgatttct gtggcttgtt gcggggtaag atccgggact cgtaagacga
    58081 aaagcttatc actatgcttt atggcttttt taagggaaac aatctggacg ccagcgcctg
    58141 tcgcttcggc aacgttatta tctcccaaat agattgcaac gtgactcaca gaggaagtgc
    58201 tgaatacgcg gatatcaaat gaggttaccc caaggcttga ggagaaaagc aaatcgccgg
    58261 gtttgagatc tggttccgtt atttctttta ttgattgttc ggtgaaagag cttggatgct
    58321 ggaatttaac agcccatgtt ttcgcctcgg catttaccac tgtggctgat gagtctggct
    58381 ggctgatatc aacggtacag gccgaaagta aaagaaagca ggggagaagc aggcggcagt
    58441 acgcctttgg tttatccatt ttatacaatc catgtaaaaa agggccccga aattcaggac
    58501 cctttctggc atcagccttt aatctgtttc accagataat cgacgatgtc accagtctta
    58561 attaactgtt tctcgccgtt acgacgatat ttatattcga tatcatcgtt gtcgaggtta
    58621 cggtcgccca gcacaatagt gtgcggaata ccgatcagtt ccatatcagc aaacatcacg
    58681 cccggacgct ctttgcggtc atccagcagc acttcgatac cttgtgcgcg cagttcgctg
    58741 tacagtttct cagcaagttc ctgtacgcgg aaggatttgt gcatgttcat tggcagaatc
    58801 gccaccagga acggcgcgat agcgtcaggc catacgatgc cgcgttcgtc gtagttctgc
    58861 tcaatcgccg ctgctaccac acgcgttacg ccgataccgt agcaacccat cgtcaggatt
    58921 tggttacggc catcttcacc ctgtacggag gctttcagtg cttcggagta cttggtaccc
    58981 agctggaaga tgtgaccgac ttcgatacca cgtttgatca gcagcgtacc ctgaccatcc
    59041 gggcttggat cgccagccac cacgttgcgg atatcagcaa tttccggggt agcgacatcg
    59101 cgatcccagt tgataccgaa gtagtgttta ccatcgatgt tagcaccagc agcgaaatca
    59161 ctcatcgccg caacggtacg gtcaatcacc accggaatcg gcatgtttac cggacccagt
    59221 gaacccggac cggctttaac cacggcacga atttcttctt cggtcgcaaa agtcagcggg
    59281 ctggcaacct gcggcagttt ttctgcttta acttcgttca gctcgtggtc accgcgcacc
    59341 agcagcgcaa ccagcgggaa gctactgcct tcaacggctt taaccagcag agtcttaacc
    59401 gttttctcaa tcggcagatt gaactgttca accagttccg cgatggtttt cgcgttcggc
    59461 gtatcaacca gcgtcatttc ctgggtagca gcagcgcgcg gttctttcgg cgcgatagct
    59521 tctgccagtt caatgttcgc tgcatagtca gaagtatcgg agaagaccac atcgtcttca
    59581 ccgctctgcg ccagcacctg aaattcgtga gaggcgctac cgccgataga accggtatcg
    59641 gcctgtacgg cgcggaaatc cagccccatg cggctgaaga ttttgctgta ggccgcatac
    59701 attgcatcgt aggtttcctg cagggattcc tgagaagtat ggaaagagta agcatctttc
    59761 atcaggaatt cgcgggaacg catgacgcca aaacgcggac gcacttcgtc gcggaacttg
    59821 gtctggatct gatagaagtt cagcggcagc tgtttgtaag agctcagctc gttacgaatc
    59881 aggtctgtaa tcacttcttc atgagttggg ccgagtacga acggacggtc accacggtca
    59941 gcaatacgca gcaattccgg gccatactgt tcccaacgac cactctcttg ccacagttca
    60001 gatggctgaa ccaccggcat taacacctcg atcgcaccgg cgttgttcat ctcttcacgc
    60061 acgatgtttt cgactttttt cagaacgcgc acgccggtcg gcagccaggt atataacccg
    60121 gaggccagct tgcggatcat cccggcgcgc agcatcagct gatggctgat cacctcggcg
    60181 tcggcaggtg tctccttgag agtggagagc aggtattggc tagtacgcat gttgttacgg
    60241 ttccagttgg aaggtagaac aggctcaagg cgagcctggg acaaaaaaag tgatttagtt
    60301 taccagtgca aaagaaatgt caaaagagaa gggcgtgaat ttaacgcggt tccagcgcaa
    60361 agacttcaaa acctgcgtcg gtgacgcgcc agcgaacgtt aaaatcatgc agccagacgg
    60421 cataggtttt gcccgtttcc tcacctttac gataggccgg gcgcgggtcc tgcgccagta
    60481 cttcgcggat aaacagcgtt aactgcggat aacgcttctt cagcgtcaaa agctgctttt
    60541 cgacctctgc ggtaaaacac accgccattt ctgccgctgg cgcactttgc gcatagctgg
    60601 cactggcatc gggaagcgat tcagcaaagg ggagatacgg tttgatatcc actaccggcg
    60661 taccatcgac cagatccaga ctgccgagct tcagaatcac gctatcttta tggcaaacaa
    60721 cctctttcag ctctaccagc gacataccaa tcgggttagg gcgaaaagtg gagcgcgtag
    60781 cgaaaacacc cattctggca ttaccgccga gacgaggcgg acgcacagtc ggacgccagc
    60841 cgccttccat cgtttgatga aagacgaaaa ggatccataa atggctgaat gcttccaggc
    60901 cgcgaacggc gtcggcctgg ttgtagggag caatgagatg tagttcgccg ttggcgcttt
    60961 ttaccagacc tggctggcgc ggaacggcga atttttcttt atagggcgag cgaataacgc
    61021 ctatttgctc gaactggaaa ctgctcattt cgccgtgatg ttaagcgcag aaccgataca
    61081 tacagcctga cgatagcaac ctggcgtacc gctggtgact tcgcagctat gcagtaatac
    61141 cgcattggct ttcattttcg aggcgttgat ttgcatccgc ttacgtgcgg ttggaatgct
    61201 cggcggagag tcctgattag aggcctggca agagtcgcca ctgacttcac cgagatcgcg
    61261 gaacggtttg ccgactaatt cttctgcatt ggtataaatt cggaccggcg tggcgcgcgg
    61321 cgctttcggt tttgcaggct ccgctttcgg ctggggtgca gtgctttgaa cgggttcgac
    61381 aggggatctg cttaacatgg aacagccgct tagcatgagt cctactaaac agatcggtaa
    61441 agcacgcata gtatttcctc aatgtatgat caaaacgtca atattgaatc aggcgcttgt
    61501 aaaaatgaca agacgggcaa gcgcccgtcc tgaatgatat tacaaattgt ggaaacagcc
    61561 taaatattac cagcctttaa cagcgccgcc gttaaacact ttgtttgctg cttcgtaaac
    61621 ttcgtcagac tgataagcct gaacgaattt cttcacgttt tcggcgtctt tgttgtcttc
    61681 acgcgttacg atcaggttta cgtacgggga ctctttatct tcgacaaaga taccgtcttt
    61741 cgctggagtc aggccaatct ggctggcata ggtggtattg ataactgcca gagcgatttg
    61801 cgcgtcgtcc agagagcgcg gcagctgcgg tgcttccagt tcaacaattt tcagattttt
    61861 tgggttctca acaacatcaa gaacggtcgg cagcaggcca acgccatctt tcagtttgat
    61921 caagcccact ttttgcagca gcagcagtga acgaccaagg ttagttgggt cgtttggcac
    61981 ggcaacctgc gaaccatcct gcagttcatc cagtgatttg attttcttgg agtaaccagc
    62041 aatcggataa acaaaagtgt tgcctactgc gaccagtttg tagccacgat ctttcagttg
    62101 ctgatcaagg tacggtttat gctggaaggc gttggcgtcg atatcgcctt tgctcaatgc
    62161 ttcgtttggc agaacatagt cgttgaaggt taccagctca acgtccaggc catatttgtc
    62221 tttcgcaact ttctgcgcga cttctgcaac ctgctgttcg gcaccaacaa tcacgccgac
    62281 tttaatgtgg tttggatctt tttcatcctg accgcagcct accagtgcca gtgatccaat
    62341 cagggctccc actgccgcaa aggttttgaa tttgaacgcc ataccttatt ccttcttcaa
    62401 ttatttatgt tgtgttgaac gttacttgcg agtgacagcc cggacgatgc ggtcgcctgc
    62461 gaactgaatt aaataaacca gaatgaccag caataccagt accgtattca tcaccgtcgc
    62521 gttgtagccg atgtagccat actgatagcc aatctgacct aaaccaccgg caccgactgc
    62581 accacccatc gcggaataac caaccagggt aatcagggtg atagttgccg cattcaccag
    62641 acccggcaac gcttccggta acagcacttt acggacgatc tgcattggcg tggcccccat
    62701 tgcgcgggaa gcttcaatta acccggttgg gatctccagc agagcgttct cgaccatacg
    62761 ggcaataaac ggtgctgcac caacggttaa cggaacaatc gctgcctgca atccaatcga
    62821 tgtaccgaca ataacgcggg taaacggaat catccatacc agcaagataa tgaacgggat
    62881 ggaacggaaa atgttcacaa tcgcagaaat ggtacgatac aacttcgcgt tagcaataat
    62941 ttgccccgga cgcgtgacat aaagcagaac gccaaccggc agaccaatca caaagccaaa
    63001 aaaaccggat acgaaggtca ttgccagcgt ttcccatacg ccacgaacca gcagccacat
    63061 catcggctca gacataaccc agtacctcta cttttacatg gtgttcctgc agccaggcaa
    63121 tggcggcttg cgtatcttgt tgtgtgccgt gcatttcagt cagcatgatg ccgaacttaa
    63181 cgccaccggc gtaatccatc tgcgcgctaa taatgttgtt gttgacgttg aaacgacgcg
    63241 cggtttcaga aagcagtggg gcatcgaccg attgaccggt aaactccaga cgcagcatcg
    63301 gcacgcagtc agtaaatggc tccgcttgca gacgttcctg gtaatcttcc gggatatcca
    63361 gatgcagggt cgactgaata aacttctgcg ccagcggcgt tttcggatgc gagaacactt
    63421 cacttaccgt gtcctgctcg atcagttcgc cattgctgat gaccgccacg caatcacaaa
    63481 tgcgcttcac aacgtccatt tcgtgagtga tcaacagaat cgtcaaaccc agacggcggt
    63541 tgatgtcttt cagcagttcg agaatagaac gtgtcgttgc cgggtccagc gcgctggtgg
    63601 cttcatcaca cagcaatact ttgggattgc tggctaatgc acgggcaatc gccacacgtt
    63661 gtttctgccc accggaaaga ttcgacgggt agctatcatg cttatcgcca agaccaacca
    63721 atgacagcaa ttccgtcacg cgacgtttga tctcgtcttt cggtgtgttg tccagctcca
    63781 gcggcagagc cacgttgcca aaaacagtac gcgaagagag caggttaaaa tgctggaaaa
    63841 tcataccaat ctggcgacga gctttggtca actcggattc tgacagcgtg gtcaattcct
    63901 ggccatcgac cagcacgcta ccctcggttg ggcgctccag caggtttaca caacgtataa
    63961 gcgtactctt acccgcgcct gaggcaccga taacgccata aatttgtcca gctggcacat
    64021 gcaggctgac gttgttcaac gcctggatgg tgcgggtgcc ctggtggaac actttggtga
    64081 tattcgaaag ttttatcatt gattatttat tatcgtcatt aagttagtcg tggcatttcg
    64141 aatgcctgga acgggcatcg ccgtcaatga aatggatgtt aaggcatcca gacgtctaaa
    64201 tcaatcaggt ttatgcgaag agcactttct tgcaggtcga aacatgcgat actagcgtca
    64261 catgccttat taaggagcta taaaaggtgg cgaagagcgt acccgcaatt tttcttgacc
    64321 gtgatggcac cattaatgtt gatcacggct atgtccatga gatcgacaac tttgaattta
    64381 tcgacggtgt tattgacgcc atgcgcgagc taaaaaaaat gggctttgcg ctggtggtag
    64441 taaccaacca gtctggcatt gctcgcggta aatttactga agcacagttt gaaacgctga
    64501 ccgagtggat ggactggtcg ctggcggacc gcgatgtcga tctggatggc atctattatt
    64561 gcccgcatca tccgcagggt agtgttgaag agtttcgcca ggtctgcgac tgccgcaaac
    64621 cacatccggg gatgtttttg tcagcacgcg attatttgca tattgatatg gccgcttctt
    64681 atatggtggg cgataaatta gaagatatgc aggcagcggc tgcggcgagc gtgggaacaa
    64741 aagtgctggt gcgtacgggt aaacctatta cgcctgaagc agaaaacgcg gcggattggg
    64801 tgttaaatag tctggcagac cttccgcaag cgataaaaaa gcagcaaaaa ccggcgtgat
    64861 gattaaaaga tgagcggttg aaataaaaat gcatttttcc gcttgtcttc ctgaaacgac
    64921 tccctataat gcgcctccat cgacacggcg gatgtgaatc acttcacaca aacagccggt
    64981 tcggttgaag agaaaaattc tgaaattcag ggttgactct gaaagaggaa agcgtaatat
    65041 acgccacctc gcgacagtga gctgaaagcc gagtcgcaac tgctctttaa caatttatca
    65101 gacaatctgt gtgggcactc gaagatacgg attctt
//
LOCUS       DAZFFE010000012        64862 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFFE010000012 DAZFFE010000000
VERSION     DAZFFE010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.012"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..674
                     /locus_tag="ACHJ69_000951"
     CDS             <1..674
                     /locus_tag="ACHJ69_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001726558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5013998.1"
                     /translation="EIIKTILKSNSPDLSTWLIATNNYIRLSKYEYIPHINTLDEDTI
                     NQSKLSFSDDEIKSYFHKTVPNINDIPLNMLKHDGDNLYNFFVDKYINITKRERIEEL
                     KNKMVNDGWTTIDMDIYHSDFKFKALETLDVNLIISAIQHNWSIYDIQMFSDHLLSVY
                     NFSNLRDYLSNELPHLKKLDEFLNSYLNELSISFRRGAIIELKNTVKKVKESLENSIS
                     FINKN"
     gene            complement(945..1517)
                     /locus_tag="ACHJ69_000952"
     CDS             complement(945..1517)
                     /locus_tag="ACHJ69_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFO5013999.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDAA
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
     gene            complement(1591..2091)
                     /locus_tag="ACHJ69_000953"
     CDS             complement(1591..2091)
                     /locus_tag="ACHJ69_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO5014000.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDSKDSGMEYISGKQRYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAESLHEKQELPPVRLKTQS
                     YSLRLE"
     gene            complement(2088..2822)
                     /locus_tag="ACHJ69_000954"
     CDS             complement(2088..2822)
                     /locus_tag="ACHJ69_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HFO5014001.1"
                     /translation="MSDNTIPEYLQPALAQLEKARAAHLENARLMDETITAIERAEQE
                     KNALAQADGNDADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGGCDSTATAYRKAHHHLLSLYAEHDLEHALNETCEALVRAMHLSILVQENPLAN
                     TTGHQGYVAPEKAVMQQVKSSLEQKIKQMQISLTGEPVLRLTGLSAATLPHMDYEVAG
                     TPAQRKVWQDKIDQQGAELKARGLLS"
     gene            3373..3627
                     /locus_tag="ACHJ69_000955"
     CDS             3373..3627
                     /locus_tag="ACHJ69_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5014002.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPECGLSRSTIYELI
                     RKGEFPPQVSLGGKNVAWLHSEITAWMAGRIAGRKRGYDA"
     gene            3624..4214
                     /locus_tag="ACHJ69_000956"
     CDS             3624..4214
                     /locus_tag="ACHJ69_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5014003.1"
                     /translation="MMMPALQKVPFSGLPFSGICGYSFSAAAKSAAGRRNPCNSKATY
                     DAPCVFFCVAINATERQIMVWRVVCRAGMILFAIACYATESMVAQAGQPPGWPVFFEA
                     GIPTPVWAIAIERRNSGDSSYLLLEGDCLMATTLTPSHPEFVFVFAAVRRADRHPRIC
                     MLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            4207..4494
                     /locus_tag="ACHJ69_000957"
     CDS             4207..4494
                     /locus_tag="ACHJ69_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HFO5014004.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSRFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            4487..4942
                     /locus_tag="ACHJ69_000958"
     CDS             4487..4942
                     /locus_tag="ACHJ69_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014005.1"
                     /translation="MFDFPQPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPEIVWNP
                     YRRKFSILVRILTDGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWREKYLDIYPQDTVPASRAAPVAREWREISRTEPDPEITPDNSYRNYL"
     gene            5078..5398
                     /locus_tag="ACHJ69_000959"
     CDS             5078..5398
                     /locus_tag="ACHJ69_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HFO5014006.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKA
                     PVLH"
     gene            5413..7746
                     /locus_tag="ACHJ69_000960"
     CDS             5413..7746
                     /locus_tag="ACHJ69_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO5014007.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNRHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDAAAKVAAVTGSLPPADPAVTAAA
                     GAETDAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEATRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPQDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRRLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVE
                     GETLENHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATT"
     gene            8304..9341
                     /gene="rhuM"
                     /locus_tag="ACHJ69_000961"
     CDS             8304..9341
                     /gene="rhuM"
                     /locus_tag="ACHJ69_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HFO5014008.1"
                     /translation="MSDKDLTQPPAGEFIMFASGDGRVRVECRFESDTIWLSQAAMAE
                     LYDKDVRTINEHLINIFSEGELVQNSTIRKFRIVRQEGKRQVSREIDHYNLEAILAVG
                     YRVRSPRGTQFRQWATQTLQEYLIKGFVMDDERLKNPPVGSSVVPDYFDEMLERIRDI
                     RASERRVYLRMREIFALAADYQPSLKETTQFFQTIQNKLHFACTGHTAAELIHKRADA
                     SQPHMGLTSYKGEEVRKGDVTVAKNYLTQDEVSELNRVVNMWLDFAEDQARRRQQVFL
                     RDWQDKLDQFLQFNDREVLQGAGKISKKMADEKAQAEYVQFAEQQRRLKEAEGEKDIA
                     GLLQWNKESKK"
     gene            9911..10408
                     /locus_tag="ACHJ69_000962"
     CDS             9911..10408
                     /locus_tag="ACHJ69_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014009.1"
                     /translation="MGHGTCKAAKNARHLIESKEKNRSSRVKVFSTHINLSHPAASWL
                     ATQTCANNIFNLFQNNNALYLFENDNKIELRMGHDSFFSIETIDKINKENIKEIKLKN
                     ETTYVSLPNPTILITTTSTPERQFSISVLDDDNKENIPTDTAPCFTIGIPDEYTKAIE
                     LLNIK"
     gene            10586..11509
                     /gene="yicL"
                     /locus_tag="ACHJ69_000963"
     CDS             10586..11509
                     /gene="yicL"
                     /locus_tag="ACHJ69_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO5014010.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(11513..12331)
                     /gene="nlpA"
                     /locus_tag="ACHJ69_000964"
     CDS             complement(11513..12331)
                     /gene="nlpA"
                     /locus_tag="ACHJ69_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO5014011.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            12553..12846
                     /gene="yicS"
                     /locus_tag="ACHJ69_000965"
     CDS             12553..12846
                     /gene="yicS"
                     /locus_tag="ACHJ69_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO5014012.1"
                     /translation="MKPTMLLMITIFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(12887..13735)
                     /gene="nepI"
                     /locus_tag="ACHJ69_000966"
                     /pseudo
     CDS             complement(12887..13735)
                     /gene="nepI"
                     /locus_tag="ACHJ69_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(13764..14216)
                     /gene="yicN"
                     /locus_tag="ACHJ69_000967"
     CDS             complement(13764..14216)
                     /gene="yicN"
                     /locus_tag="ACHJ69_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO5014013.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVEQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(14269..15603)
                     /gene="adeQ"
                     /locus_tag="ACHJ69_000968"
                     /gene_synonym="yicO"
     CDS             complement(14269..15603)
                     /gene="adeQ"
                     /locus_tag="ACHJ69_000968"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO5014014.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDSKVVFVTTCLIAGIGSIAMGVFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALSLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVATLFALKIILVD"
     gene            15778..17544
                     /gene="adeD"
                     /locus_tag="ACHJ69_000969"
     CDS             15778..17544
                     /gene="adeD"
                     /locus_tag="ACHJ69_000969"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO5014015.1"
                     /translation="MNNSINHKFHHISRTEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGHRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIDRQP
                     VSASDFALQFTPGKRYRVIEVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(17591..18981)
                     /gene="uhpT"
                     /locus_tag="ACHJ69_000970"
                     /pseudo
     CDS             complement(17591..18981)
                     /gene="uhpT"
                     /locus_tag="ACHJ69_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
     gene            complement(19119..20438)
                     /gene="uhpC"
                     /locus_tag="ACHJ69_000971"
     CDS             complement(19119..20438)
                     /gene="uhpC"
                     /locus_tag="ACHJ69_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO5014016.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSEMLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(20448..21950)
                     /gene="uhpB"
                     /locus_tag="ACHJ69_000972"
     CDS             complement(20448..21950)
                     /gene="uhpB"
                     /locus_tag="ACHJ69_000972"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO5014017.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLMQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPDSGQHGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(21950..22540)
                     /gene="uhpA"
                     /locus_tag="ACHJ69_000973"
     CDS             complement(21950..22540)
                     /gene="uhpA"
                     /locus_tag="ACHJ69_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO5014018.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(22613..22903)
                     /gene="ilvN"
                     /locus_tag="ACHJ69_000974"
     CDS             complement(22613..22903)
                     /gene="ilvN"
                     /locus_tag="ACHJ69_000974"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO5014019.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQFDPTMFNKIAVFFQ"
     gene            complement(22907..24595)
                     /gene="ilvB"
                     /locus_tag="ACHJ69_000975"
     CDS             complement(22907..24595)
                     /gene="ilvB"
                     /locus_tag="ACHJ69_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO5014020.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(24701..24799)
                     /gene="ivbL"
                     /locus_tag="ACHJ69_000976"
     CDS             complement(24701..24799)
                     /gene="ivbL"
                     /locus_tag="ACHJ69_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO5014021.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            complement(25044..25118)
                     /gene="ysdE"
                     /locus_tag="ACHJ69_000977"
     CDS             complement(25044..25118)
                     /gene="ysdE"
                     /locus_tag="ACHJ69_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO5014022.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            25200..26384
                     /gene="emrD"
                     /locus_tag="ACHJ69_000978"
     CDS             25200..26384
                     /gene="emrD"
                     /locus_tag="ACHJ69_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO5014023.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLMVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLIPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(26392..26889)
                     /gene="yidF"
                     /locus_tag="ACHJ69_000979"
     CDS             complement(26392..26889)
                     /gene="yidF"
                     /locus_tag="ACHJ69_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO5014024.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQLGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDTTLQKWCGSEKS
                     QPRRDCQACDWHRLCPHAREDKPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(26886..27248)
                     /gene="yidG"
                     /locus_tag="ACHJ69_000980"
     CDS             complement(26886..27248)
                     /gene="yidG"
                     /locus_tag="ACHJ69_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO5014025.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(27238..27585)
                     /gene="yidH"
                     /locus_tag="ACHJ69_000981"
     CDS             complement(27238..27585)
                     /gene="yidH"
                     /locus_tag="ACHJ69_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO5014026.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(27645..29138)
                     /gene="yidJ"
                     /locus_tag="ACHJ69_000982"
     CDS             complement(27645..29138)
                     /gene="yidJ"
                     /locus_tag="ACHJ69_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5014027.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARADEPFLMVISYDEPHHPFTCPVEYLEKYTDFYYELGEKA
                     EDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVLVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIHFADVRSKMHDALLDYMDKIRDPFRSYQWNLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(29135..30850)
                     /gene="yidK"
                     /locus_tag="ACHJ69_000983"
     CDS             complement(29135..30850)
                     /gene="yidK"
                     /locus_tag="ACHJ69_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO5014028.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQLSDG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNVEPQQLVTVGRKFGFFIAIVSVMV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLMIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAIISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            31017..31883
                     /gene="yidL"
                     /locus_tag="ACHJ69_000984"
     CDS             31017..31883
                     /gene="yidL"
                     /locus_tag="ACHJ69_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO5014029.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNDEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEVYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMHAVGKKDPQRRQIEKLIATLHANLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDR"
     gene            complement(31973..33634)
                     /gene="yidE"
                     /locus_tag="ACHJ69_000985"
     CDS             complement(31973..33634)
                     /gene="yidE"
                     /locus_tag="ACHJ69_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO5014030.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGVGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMAMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESTRTNGGALIRTINIRVENPNLHN
                     LAIKDVPILNGDKVICSRLKREETLKVPSPETVIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVARVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASSD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFIHTLVDGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(33832..34260)
                     /gene="ibpB"
                     /locus_tag="ACHJ69_000986"
     CDS             complement(33832..34260)
                     /gene="ibpB"
                     /locus_tag="ACHJ69_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO5014031.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(34372..34785)
                     /gene="ibpA"
                     /locus_tag="ACHJ69_000987"
     CDS             complement(34372..34785)
                     /gene="ibpA"
                     /locus_tag="ACHJ69_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO5014032.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(34779..34851)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            35091..35423
                     /gene="yidQ"
                     /locus_tag="ACHJ69_000988"
     CDS             35091..35423
                     /gene="yidQ"
                     /locus_tag="ACHJ69_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO5014033.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVLDTILLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(35425..36639)
                     /gene="yidR"
                     /locus_tag="ACHJ69_000989"
     CDS             complement(35425..36639)
                     /gene="yidR"
                     /locus_tag="ACHJ69_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO5014034.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAYVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIVE
                     SGKVSNLDAMDITASYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSNEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPKDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EDGQLWITETDR"
     gene            36740..37840
                     /gene="cbrA"
                     /locus_tag="ACHJ69_000990"
     CDS             36740..37840
                     /gene="cbrA"
                     /locus_tag="ACHJ69_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO5014035.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKLNTAYRRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(37801..39138)
                     /gene="dgoT"
                     /locus_tag="ACHJ69_000991"
     CDS             complement(37801..39138)
                     /gene="dgoT"
                     /locus_tag="ACHJ69_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5014036.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAQMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFIGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(39213..40361)
                     /gene="dgoD"
                     /locus_tag="ACHJ69_000992"
     CDS             complement(39213..40361)
                     /gene="dgoD"
                     /locus_tag="ACHJ69_000992"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFO5014037.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELSDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(40358..40975)
                     /gene="dgoA"
                     /locus_tag="ACHJ69_000993"
     CDS             complement(40358..40975)
                     /gene="dgoA"
                     /locus_tag="ACHJ69_000993"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO5014038.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(40959..41837)
                     /gene="dgoK"
                     /locus_tag="ACHJ69_000994"
     CDS             complement(40959..41837)
                     /gene="dgoK"
                     /locus_tag="ACHJ69_000994"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO5014039.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDCRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMS
                     GELHHLLLNHSLIGAGLPPQENAADAFAAGLERGLNAPAVLPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(41834..42523)
                     /gene="dgoR"
                     /locus_tag="ACHJ69_000995"
     CDS             complement(41834..42523)
                     /gene="dgoR"
                     /locus_tag="ACHJ69_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFO5014040.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            42802..43458
                     /locus_tag="ACHJ69_000996"
     CDS             42802..43458
                     /locus_tag="ACHJ69_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010377884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014041.1"
                     /translation="MKLNLKGFFKAASLCSLAFALTGCITWGLVSNTASHPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDEVVNMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPRGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(43502..44314)
                     /gene="yidA"
                     /locus_tag="ACHJ69_000997"
     CDS             complement(43502..44314)
                     /gene="yidA"
                     /locus_tag="ACHJ69_000997"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO5014042.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(44429..44827)
                     /gene="yidB"
                     /locus_tag="ACHJ69_000998"
     CDS             complement(44429..44827)
                     /gene="yidB"
                     /locus_tag="ACHJ69_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO5014043.1"
                     /translation="MGLFDDVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQSNQSVSGEQVESALGTNAVFDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            44945..45046
                     /locus_tag="ACHJ69_000999"
                     /pseudo
     CDS             44945..45046
                     /locus_tag="ACHJ69_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(45067..47481)
                     /gene="gyrB"
                     /locus_tag="ACHJ69_001000"
     CDS             complement(45067..47481)
                     /gene="gyrB"
                     /locus_tag="ACHJ69_001000"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO5014044.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYDILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTETDLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(47510..48583)
                     /gene="recF"
                     /locus_tag="ACHJ69_001001"
     CDS             complement(47510..48583)
                     /gene="recF"
                     /locus_tag="ACHJ69_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO5014045.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPQAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKEIEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(48583..49683)
                     /gene="dnaN"
                     /locus_tag="ACHJ69_001002"
     CDS             complement(48583..49683)
                     /gene="dnaN"
                     /locus_tag="ACHJ69_001002"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO5014046.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(49688..51091)
                     /gene="dnaA"
                     /locus_tag="ACHJ69_001003"
     CDS             complement(49688..51091)
                     /gene="dnaA"
                     /locus_tag="ACHJ69_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO5014047.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            51388..51468
                     /locus_tag="ACHJ69_001004"
     CDS             51388..51468
                     /locus_tag="ACHJ69_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014048.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTRQPV"
     gene            51698..51838
                     /gene="rpmH"
                     /locus_tag="ACHJ69_001005"
     CDS             51698..51838
                     /gene="rpmH"
                     /locus_tag="ACHJ69_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO5014049.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            51855..52214
                     /gene="rnpA"
                     /locus_tag="ACHJ69_001006"
     CDS             51855..52214
                     /gene="rnpA"
                     /locus_tag="ACHJ69_001006"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO5014050.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            52178..52435
                     /gene="yidD"
                     /locus_tag="ACHJ69_001007"
     CDS             52178..52435
                     /gene="yidD"
                     /locus_tag="ACHJ69_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO5014051.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            52438..54084
                     /gene="yidC"
                     /locus_tag="ACHJ69_001008"
     CDS             52438..54084
                     /gene="yidC"
                     /locus_tag="ACHJ69_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO5014052.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            54190..55554
                     /gene="mnmE"
                     /locus_tag="ACHJ69_001009"
     CDS             54190..55554
                     /gene="mnmE"
                     /locus_tag="ACHJ69_001009"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO5014053.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            55796..55870
                     /gene="tnaC"
                     /locus_tag="ACHJ69_001010"
     CDS             55796..55870
                     /gene="tnaC"
                     /locus_tag="ACHJ69_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO5014054.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            56091..57506
                     /gene="tnaA"
                     /locus_tag="ACHJ69_001011"
     CDS             56091..57506
                     /gene="tnaA"
                     /locus_tag="ACHJ69_001011"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO5014055.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            57598..58845
                     /gene="tnaB"
                     /locus_tag="ACHJ69_001012"
                     /pseudo
     CDS             57598..58845
                     /gene="tnaB"
                     /locus_tag="ACHJ69_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
     gene            58977..60152
                     /gene="mdtL"
                     /locus_tag="ACHJ69_001013"
     CDS             58977..60152
                     /gene="mdtL"
                     /locus_tag="ACHJ69_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO5014056.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSALFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIAVLMLSLFILKETRPAAPTTSDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            60127..61086
                     /gene="yidZ"
                     /locus_tag="ACHJ69_001014"
     CDS             60127..61086
                     /gene="yidZ"
                     /locus_tag="ACHJ69_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO5014057.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMSVTPSAVSKSLA
                     KLRMWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNQLLDKPLHETPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSRNPKIVWLRETIKNLYASMA"
     gene            61243..61992
                     /gene="yieE"
                     /locus_tag="ACHJ69_001015"
     CDS             61243..61992
                     /gene="yieE"
                     /locus_tag="ACHJ69_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO5014058.1"
                     /translation="MATHFARGILTEGHLISVRLPSLCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            62014..62580
                     /gene="yieF"
                     /locus_tag="ACHJ69_001016"
                     /gene_synonym="chrR"
     CDS             62014..62580
                     /gene="yieF"
                     /locus_tag="ACHJ69_001016"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO5014059.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEINALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQSTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(62634..63971)
                     /gene="adeP"
                     /locus_tag="ACHJ69_001017"
     CDS             complement(62634..63971)
                     /gene="adeP"
                     /locus_tag="ACHJ69_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO5014060.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMSVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            64138..64803
                     /gene="yieH"
                     /locus_tag="ACHJ69_001018"
     CDS             64138..64803
                     /gene="yieH"
                     /locus_tag="ACHJ69_001018"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO5014061.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
BASE COUNT        16092 a        17258 c        16197 g        15315 t
ORIGIN      
        1 aagaaataat aaaaacaatt ttaaaatcaa attctccaga tctatctaca tggttaattg
       61 ccacgaataa ctatattaga ctctcaaaat atgaatatat acctcatata aacaccctag
      121 atgaagacac cattaatcaa agcaaactta gcttcagtga cgatgaaata aaatcatatt
      181 ttcataaaac cgtgccaaac atcaatgaca tcccactaaa tatgctaaaa catgatggag
      241 ataatttata taactttttt gttgacaaat atattaacat aacaaaaaga gaaagaatag
      301 aagaattaaa aaacaagatg gttaacgatg gttggaccac tatcgatatg gatatatatc
      361 attcagattt caagtttaaa gcactggaaa ctttagatgt aaatttaatc ataagtgcca
      421 tacaacataa ttggtccatt tacgatattc aaatgttttc agaccatcta ttatctgtat
      481 acaacttttc taaccttcgc gattatctat ctaacgagct acctcatcta aaaaaactag
      541 atgagttctt aaattcttac ctgaatgaac tttcaatttc ttttcgtcgc ggagccatta
      601 ttgagttaaa aaacaccgtg aaaaaagtaa aggagtcact agagaacagt atttcattca
      661 taaataaaaa ctgaacacat ccatgtaaaa acaaaaaact ctccagttca gtttctttct
      721 ctggctggag agtcaggtta tttcctgcat acatgcgcgc agtgctttcc ccgcctcgcc
      781 cgcccgcttt gcggggcggt tttaatgcag tttcactgac acgctcaggc cgcgccggga
      841 atggcgcggt ctgcagaaaa tgaggcagga aaacgcatgc aaagccatgc accttatcga
      901 tgcatggctt ttttcagtaa aaacaggcgg attttcgggg aattttacac agactgacgt
      961 gatgccagtt gcgcactttt acgcgaaaaa atcatgttct gcgcaggggt gaatttttca
     1021 cggctgtcat ccaccgaagc cgcgtcaggc ctgaatccga tggtcgttaa aatgtcgcta
     1081 tcctgtgcgg aataattaat tttttccccc ttcgcaagcc aggacaggag ggcttcacgc
     1141 agggcatctg tggcacgctg tatggcacag tttcgggcaa tggccgtcag ctcactgtag
     1201 cccatcagct ccggtgccag tgccgccgcc agtgctgtgc cgtgctgctg cataaaatca
     1261 ttcagccggt cgcggatgct gatgtgctga acggcttcat gcgaacgaat ataacgacca
     1321 gcggcctgat tcacctgcca ttttctgact tcgataatat tgcgtaattc gtccaggcga
     1381 ctgacgtttc tgccttctcc tgacagaagc cgcagatatt cctgttcggc cgctgccagc
     1441 tcatttttgc gttgcagcca tgctgctttg ttattctgac aggtgtcaaa ggcctgctgt
     1501 aaggctgtgc tttccatcgt tatctctttc tcatcatgct gaagaataaa aatacggtgt
     1561 gcggcgacgg ccggtgttaa ccggcagccc tcattccaga cgcagcgaat atgattgtgt
     1621 ttttaagcgt actggcggca gttcctgttt ttcatgcagg ctttctgcca gttcgtccgg
     1681 cgtgaccggg cggacaatga aacggttgat ggtctgaagc gttttaaaca ccagaccaca
     1741 gcccggatcc gtgcacacat aaaaacgctc ggtgacttcc tgagacagac gccgcgatgt
     1801 tcttgacagt gcaaggcctt tacatctccg acaacaatat ccggtaacaa gcattctttt
     1861 cgggcgtttc atactgccgg aggctgacgt cagtgaatcg cggtatctct gtttgcctga
     1921 aatgtattcc attcctgaat ctttactgtc agagaaaaag ctttcactcg cttcaaatgt
     1981 cgcagagcaa taaatattcc ggcactgtgc aatcattatc ttggtgccat cgtccatgaa
     2041 atgtgcgcga cgggtgtgag caacatgtcc acacgacggg cagtaaatca tgacagcagt
     2101 cctctggcct taagctctgc tccctgctgg tctattttgt cctgccacac cttgcgctgt
     2161 gccggtgtgc ctgccacctc ataatccatg tgcgggagtg ttgccgctga cagtccggtc
     2221 agccggagaa ccggctcgcc ggtgaggctg atttgcatct gtttaatttt ctgttccagc
     2281 gatgatttca cctgctgcat gacagccttt tccggtgcga cgtagccctg atggccggtg
     2341 gtgttggcga gcggattttc ctgtaccaga atgctcagat gcattgcccg gacaagcgcc
     2401 tcacaggttt cattcagggc gtgttccaga tcatgctctg catacagact cagaaggtga
     2461 tgatgtgcct tccggtaggc ggtggccgtg ctgtcacacc cccctttcag gcgttcacgt
     2521 tcgaaattca gcaccacggc cagattgtca tattcctgta ccagctcccg gcgtgccacg
     2581 cgctcaatgt ggcgctgttt cagctcgtcg ctcaggacac caccggctgc acgaaaggcc
     2641 gtgcgccagt cgtcagcgtc gtttccgtcg gcctgcgcca gcgcattttt ttcctgctct
     2701 gcccgttcaa tggccgtgat ggtctcatcc atcaggcggg cgttctcaag atgggcggct
     2761 ctggcctttt ccagttgtgc cagcgcgggt tgcagatatt cagggatggt gttgtcagac
     2821 attttccggc tcctcgtcac ttcaggttaa gaaaattgtg acgtacaccg gacaacaaca
     2881 cgacgcattg cagatgtgcc agccctgaca caggagactc atcctcagac cggcaagcca
     2941 ggaaaaggtc gcaggaaaaa ccggcttact gtttgttttt ttatatttta ctgttcacct
     3001 ctgttcacct taataaaaaa gataagtaat acagtaagtt aaagggtgaa caatcgcagt
     3061 aatgactgtt caccgtctgt tcaccactgt tcacccgctc atggactttt tgtgctgttt
     3121 actactgttt atttttatta attcactgaa ataaataaga aaaaacaatt tgtatttcac
     3181 tataaaaaat tccaggtcct tcagaaccct ttgagaccct tccagtccag atggataaaa
     3241 aacacacaat cattgtaagg cagcctgaac aaatccccct gttgcgtctg ctgaaaatat
     3301 tcacaaaata aagcgctacc cgaagccgga cggacttatc cggtgctgta tggacattaa
     3361 cgaggtagcc cgatgcaagc tgttttttct tccccgtctc ccgcccctgt gacgccactg
     3421 atgccgctgc cggacatcac gcaggagcgt tttttacgtc tgccggaatg cggcctgtca
     3481 cgctcgacca tctacgaact catccgtaag ggggaatttc cgccgcaggt gagtcttggc
     3541 ggtaaaaatg tggcctggct gcactctgaa atcaccgcat ggatggccgg gcgcattgcc
     3601 ggacgcaaac gggggtacga cgcatgatga tgcccgctct gcaaaaagtc cctttttctg
     3661 gcttgccttt ttccggcatt tgcggatata gtttttccgc tgccgcaaaa tcggcagccg
     3721 ggcgtaggaa cccgtgtaac tcgaaggcga catatgacgc gccatgcgtc tttttttgtg
     3781 tcgcaatcaa cgccacagag cgccagatta tggtgtggcg tgtggtttgc cgtgcaggta
     3841 tgatcctgtt cgcaatcgca tgttatgcca ctgagtcaat ggtagctcag gcggggcagc
     3901 ctccgggctg gccggtattc ttcgaggccg gtattcctac ccccgtctgg gctatcgcca
     3961 tcgagcgtag gaactccggc gatagcagtt atttgctact cgaaggagac tgccttatgg
     4021 ctacaaccct taccccttca caccctgaat ttgtctttgt gtttgcggct gtccgtcgcg
     4081 cagaccgtca tccccgtatc tgcatgcttc gcaccgtcgc cggtgatgaa cgcagcgccc
     4141 gccgttccct tgtccgtgac tatgtgctct cccttgctgc ccgtctgccg gtggtggagg
     4201 tgtcccgtgc gtaataaaaa agcccctcag accgtctcag cgcgtcatga cgcccgtgaa
     4261 cacctcagca ttgaggctta ccataagctc aaccgcgcca gcgcagtatc ccggtttgtt
     4321 gggggtgatt tgattcaccg tgaactctcc ggcctgcatc agctctacat tccgcatatt
     4381 ttcagctacc tgaatgaaga tattgatttt gtgctgaatg agctgaaagc caaaggcctg
     4441 tgccgcgatt ttctcgccca gcagaaagac cggggagaca ggacacatgt ttgattttcc
     4501 ccagcccggt gagatttacc gttctgccgg ttttcccgat gtggccgtgg tcggcattct
     4561 ggaagacggt attccgtggg aaatgccgta ccgctgtccg gaaattgtct ggaacccgta
     4621 ccgccgaaaa ttcagtatcc ttgtgcgtat cctcactgac gggcgcacca cagacatccc
     4681 gctggggcgt tttctgcggg aatttacctg tgaccgtcct gacctgttca aacgcagccc
     4741 cgtaaaccgg catgcggtac tgaaagaaat ggccggagac ccggaattac agaaatggcg
     4801 ggagaaatat ctggatattt acccgcagga cactgttccg gccagccggg cggcaccggt
     4861 ggcgcgggaa tggcgggaaa tttcccgcac ggagcctgac ccggaaatca ccccggataa
     4921 cagttaccgc aattatctgt aattaaaaaa cgacacccga aaaattaaat gtgcgtattc
     4981 gcgcagggat acgcacgtct tcaggagacg cagatatgcc ttatcagtta atgcaaccgg
     5041 cacggaatgc agtcatctgt cacagggagg aaaacaaatg aaaaccccct taccgcccgt
     5101 cttacgcgca gccctttacc gtcgcgctgt cgcctgtgcc tggctgaccg tgtgcgaacg
     5161 tcagcaccgc tacccgcatc tcacccttga atcactggag gcggccatcg ccgctgagct
     5221 ggaaggcttt tatctgcgcc agcacggtga ggaaaaaggg cgtcagatag cctgtgccct
     5281 gctggaagat ttaatggaat ccggccccct gaaggccgcg ccgtcgctgt cctttctcgg
     5341 gctggttgtg atggatgaac tctgtgcccg tcacataaaa gcgccggtac tgcactgaag
     5401 gagaacaaca ccatgaaaat gaacgtaacc gctaccgtca gccatgcgct cggccactgg
     5461 ccgcgtattc tcccggcgct ggggattcag gtgctgaaga accgtcatca gccctgtccg
     5521 gtctgtggcg ggagtgaccg tttccgtttt gatgacaggg aggggcgcgg cacctggtac
     5581 tgcaatcagt gtggtgccgg tgacggcctg aaactggttg aaaaggtgtt tggtgtttcc
     5641 ccgtccgatg cggccgcaaa ggtggctgcc gtgaccggca gcctgccccc ggctgacccg
     5701 gcagtgacgg ctgccgccgg tgctgaaaca gacgctgccc ggaagaacgc cgccgcactg
     5761 gcacaaaccc tgatggcgaa aacccgtccc ggaaccggta acgcctacct gacccgcaag
     5821 ggctttcccg gccgggaatg ccggatgctg accggcacac acagagccgg tggcgtgagc
     5881 tggcgtgccg gtgaccttgt ggtgccactg tatgacgaca gcggcgaact ggttaacctt
     5941 cagttaatca gtgctgacgg ccgtaagcgc accctgaaag gcggacaggt caggggcacc
     6001 tgtcacaccc ttgaaggaca gaatcaggcc ggaaaacgtc tgtggatagc ggagggatac
     6061 gcgaccgcac ttaccgtgca tcacctgacc ggtgaaacgg tgatggtggc gctttcttcc
     6121 gtgaacctcc tttctctggc cagccttgcc cggcagaagc atccggcctg tcagattgtc
     6181 cttgccgcag accgtgacct cagcggtgac ggccagaaaa aagccgccgc agccgcagat
     6241 gcgtgtgaag gtgttgttgc cctgccgccg gtcttcggtg actggaatga tgccttcacg
     6301 cagtacggcg gggaagccac ccgtaaggcc atttatgatg ccatccggcc accggctgaa
     6361 agcccgttcg acaccatgag cgaagcggag ttttccgcca tgagtaccag cgaaaaggcc
     6421 atgcgtatct atgagcatta cggcgaggcg ctcgcggtcg atgccaacgg ccagcttctg
     6481 tcccgctatg aaaatggtgt ctggaaggtg ctgccgccac aggactttgc ccgggatgtg
     6541 gccgggctgt ttcagcgtct gcgcgcgccg ttctcctccg ggaaggtggc ctccgtggtg
     6601 gacaccctga agctgattat tccgcagcag gaagccccct cccgccgcct gattggcttt
     6661 cgtaacggcg tgctcgacac gcagaacggc acgttccacc cgcacagtcc gtcacactgg
     6721 atgcgcaccc tgtgcgatgt ggatttcacc ccgccggtgg aaggggaaac gctggaaaac
     6781 cacgcccccg cgttctggcg ctggcttgac cgtgccgccg gtggccgtgc ggaaaaacgc
     6841 gacgtgattc tggccgcact gtttatggtg ctggcaaacc gctacgactg gcagctcttt
     6901 ctggaggtga ccggtcccgg cggcagcggc aaaagtatca tggccgaaat cgccaccctg
     6961 ctggccgggg aggataacgc cacatcggcc accattgaga cgctggaatc cccgcgtgaa
     7021 cgtgccgcgt taactggctt ctcactgata cgcctgccgg accaggaaaa atggagcggc
     7081 gacggtgccg gactcaaggc catcaccggc ggcgatgcgg tgtccgtgga cccgaaatac
     7141 cgggatgcgt actccacgca tatcccggcg gtgattctgg ccgtgaacaa taacccgatg
     7201 cgcttcaccg accgcagcgg cggcgtgtca cgccggcggg tgattattca cttcccggaa
     7261 cagatagccc cgcaggagcg cgacccgcag cttaaggaca aaatcacccg cgagctggcg
     7321 gtcatcgtgc gtcacctgat gcagaaattc agcgacccga tgctcgcccg gtcactgctt
     7381 cagtcccagc agaactcaga cgaggcactg aacatcaaac gggatgccga cccgacgttt
     7441 gattttatcg gctatctgga aaccctgccg cagaccagcg gcatgtatat ggggaacgcc
     7501 agtatcatcc cgcgtaatta ccgtaaatac ctctatcacg cctatctggc ctacatggag
     7561 gcaaacggct accggaatgt actcagtctg aaaatgttcg ggctggggct gccggtgatg
     7621 ctgaaggaat acgggctgaa ttacgagaag cgccatacca aacaggggat acagaccaac
     7681 ctgacgctga aagaggaaag ctacggcgac tggctgccaa aatgtgacga ccctgcaaca
     7741 acctgaccct catgactcat ctgaccggca tctgccggtc ttttttcatc cctgaatccc
     7801 ccgaaggtga acaatccact gttcaccctt caccgtatat tcacccgtta tcacactgaa
     7861 attaaaagag aaaaacgaaa ggtgaacagt gtgaacaatc aaatcaaaaa aaactttttt
     7921 cttcctgagt gatttcagtg cggaggatta atcaccggta tgagtcacat cggcagaatg
     7981 ccggaggtga agaatcgaat gttcaccctt cacccattat tcaccaccta tcaaactgaa
     8041 ataaaaggag aaaacagaaa ggtgaacagt gtgaacagtt cttccgaaaa aaaatttttt
     8101 tcctgagtta acgtgcgtga agatacgtgt aacaattaat cgatagatat tatatgtata
     8161 aggatgtgta taaatgaatt agaatagaaa aatataaacg atttgaatca agtggttata
     8221 tggcaatttt gactcctgtg atcttccgcc aaaatgcctc tcctgaactc tcccgacgtc
     8281 aacaacactc cttcggaggc gacatgtcag acaaagactt aacccagccc ccagcaggcg
     8341 agtttattat gtttgccagc ggtgacggaa gagtgcgtgt tgaatgtcgc tttgagtcag
     8401 acacaatctg gttatcccag gcggcaatgg ctgaactgta cgataaagat gttcgcacta
     8461 ttaacgaaca tttaatcaat atattttccg aaggtgaact tgttcaaaac tcaaccatcc
     8521 ggaaattccg gatagttcgc caggaaggaa aacgtcaggt ttccagagag atagaccatt
     8581 acaacctcga agccattctc gctgtcggct atcgcgttcg ctcgcctcga ggcacgcaat
     8641 tccgccagtg ggccacccag acgcttcagg aatacctgat caaaggtttt gtgatggacg
     8701 atgagcggct gaagaatccg cctgtcggtt catcggttgt gcccgactat tttgatgaga
     8761 tgcttgaacg catccgcgat attcgcgcca gcgagcgtcg ggtatatttg cggatgcggg
     8821 agatctttgc tttagctgcc gactatcagc catcgcttaa agaaaccacg caattttttc
     8881 aaaccatcca gaataaactg cattttgcct gtaccgggca taccgctgcg gaactcatcc
     8941 acaaacgagc agatgccagc cagccgcaca tgggcttgac cagttataaa ggtgaagagg
     9001 tacgtaaggg cgacgtgacg gtggcaaaaa attatctcac ccaggatgag gtcagcgaac
     9061 ttaatcgcgt ggttaatatg tggctggact ttgccgagga tcaggcccgt cgtcgtcagc
     9121 aagtcttttt gcgcgactgg caggataagc tggatcagtt cctgcaattt aacgaccgag
     9181 aggttctaca gggcgctggt aaaatcagca agaaaatggc cgatgaaaaa gcgcaggcgg
     9241 agtatgttca gtttgccgaa caacaacggc gcttaaaaga agccgaaggc gagaaggata
     9301 tcgctggttt gctacagtgg aataaagagt cgaaaaagta gcccggaatc cccccaaacg
     9361 ccatacgcaa aaccatttgg gggtacttat ggggttactc atttatcaaa ttcaaaatat
     9421 aaacattaaa tcaataaatt aaccatcaaa tgcgactcct gtgatcgcca aaatgcctct
     9481 tcagtgatcc ccatcctgct tatttctgag caacctgacc ctgctgatgt atctggtagt
     9541 atgacagagt gattcgtttt tcttgccgag tgtacgaaaa cgtcttcctt gccaccgact
     9601 atcaaccgtc actcaaaaaa acctctcttt ttttcaagct atcaagaata agttgcattt
     9661 tatctgcacc ggacaccagc catccatata tggcttttac ctgctacaaa gatgaagaag
     9721 tgcataaatg tgatgttaaa acaatcagag aaaaattaca tgcgcaataa tttcactata
     9781 acattataat ccatggtgaa aataacactg cacaaggatt gtcatcatca aactcatcgg
     9841 aactattttt tctgtcgtca ttggtgtaat actgattgta agtatccttt caggagatta
     9901 tttttgccgt gtggggcatg gaacttgtaa agcagccaaa aacgctaggc atttaattga
     9961 aagtaaagag aaaaacagaa gtagtcgcgt caaagtcttt agcacacata ttaatttaag
    10021 tcatccagcg gcatcctggc tagcaactca aacttgcgct aacaatatat tcaatctgtt
    10081 tcaaaacaat aatgcccttt atttattcga aaacgataat aaaattgaac tgcgaatggg
    10141 gcatgattct tttttctcta ttgaaacgat tgataaaatt aacaaagaaa acatcaaaga
    10201 aataaaacta aaaaatgaga caacatatgt ttctcttcct aatccaacca ttttaataac
    10261 cacgacatca acacctgaac gacagttttc tatttctgtt ctggatgatg acaacaaaga
    10321 aaatatacct accgataccg caccttgttt tacaattggt attcctgatg aatataccaa
    10381 agccatagag ttattaaaca ttaaataatt aaatttcata ttgagttttt agtgagtttt
    10441 tttaaatacc atatgaataa ttgttatatg tcattatttc ctccattcac taaaactgat
    10501 tgattactct actccctcac cgtcatcggt agtggtatcc ttaacgtata ttgatgaaaa
    10561 gacaaaacag tatgcagagg gaaaaatggg ttccaccaga aaggggatgc tgaacgttct
    10621 gattgccgcc gtgttgtggg gaagttcagg ggtctgcgcg caatacatca tggagcaaag
    10681 ccagatgtcg tcgcagtttt tgactatgac gcgtttgata ttcgccggtt tgattctgct
    10741 gacgctatca tttgttcatg gtgataaaat cttttctatt attaacaatc acaaagatgc
    10801 cattagccta ctgatttttt ccgtggttgg cgcgctaacc gtacagctca cttttttgct
    10861 aaccatcgaa aaatcgaacg cagccacggc aacggtgctg caattcctct caccgactat
    10921 tatcgtcgcc tggttctcac tggtgcgtaa atcccgcccg ggcattctgg ttttctgcgc
    10981 tattttgaca tcgctgatcg ggactttttt attggtgaca cacggtaatc cgacatcatt
    11041 atcgatctct cctgccgcgt tattctgggg cattgcctcg gcgtttgctg ctgcattcta
    11101 taccacctat ccctcaacgc tgattgcccg ctatggcacg ttaccggtcg tcggctggag
    11161 tatgctgatt ggcggtctga tcctgctgcc cttttatgcc agacaaggga caaactttgt
    11221 cgttaacggc agtttgattc tggcgttttt ttatttggtg gtcattggta cgtccctgac
    11281 atttagtctg tacctgaaag gagcacaatt aattggcggt ccaaaagcca gcattttaag
    11341 ctgtgcagaa ccattaagta gcgcgctgct ctctttgctg ttgctgggaa tcaccttcac
    11401 attgccggac tggctgggaa cgctgctgat tctgtcatcg gtgattttga tttcaatgga
    11461 ttcccgccgc cgcgccagaa aaataaatcg tccggcgcgg catgagtgag aattaccagc
    11521 caggcaccgc gccaccgtta aagatggttt ctgccgcttt agcgacttcg ggtgattgat
    11581 aagattgcag aaattccttc acgttttctg cattcttatt atcttcccgt gccaccagaa
    11641 tattcacata cggcgaattt ttatcttcaa taaatacgct gtcgttaacc ggagaaagtc
    11701 ctgtttgctg gatgtaagtg gtgctgataa tcgctacatc cactttcggg tcgtccagta
    11761 cacgtggcaa ctgtgcccct tccagctcca ttatctgcaa atggcgtgga ttatcggtta
    11821 tatccaacgc ggtaggtaat aacccttttc cttctttcag agtaattagc ttctcttttt
    11881 gcagcagtaa aagcgcgcga ccaaggttag tcgggtcatt ggggatcgcc actgtcgcgc
    11941 cttcttttat ttgcgccacg gttttgattt ttttggaata acccgccatg gggaagacaa
    12001 acgtatttcc caccgctacc agtttataac catgcgcctg attatcttgt tcaagaaagg
    12061 gtcgatgctg gaagacgttc gcatcaagct cgccatgatt tgtcgcatcg ttcggtagca
    12121 acgaaccgct aaatcctacc agttcaacat cgaggccata tttctctttt gctacctttt
    12181 tagcgacttc cgcgacatct tgttctgcgc cattaataac gccaacttta atatgctttt
    12241 catcgctgct gctctggtcg caacctgcca gcagaactcc tgccagtaat aatgcggccc
    12301 ccgcccgtag atgatgtgtt gtcagtttca tttttttatc cttttattaa tgattacctg
    12361 actatagcgg taaggtcgct gcagtttaaa aaacgaaacg ctatcgataa gaataaaaag
    12421 gaataaaagt ggaatataaa gtctggcact tcttactgac cacgcaaaca cctttcgtac
    12481 agcatgatga gagcggcaaa agcaaactac acttatgctt cattcgatgc acatttgcag
    12541 aaggttgccg ctatgaagcc aacgatgcta ctcatgatta ccatattcct gatttttcca
    12601 gccatttctc aggccgagtc gccattcagt tctctacagt cggcaaaaga gaaaaccacg
    12661 gtattgcaag atttacgtaa aatttgcacg ccacaggcgt cattatcaga tgaagcgtgg
    12721 gaaaagttaa tgttgtcaga tgaaaacaat aaacaacata tacgcgaggc catcgtggcg
    12781 atggaacgta ataatcagag caattattgg gaagcgttgg gtaaggtaga atgcccggat
    12841 atgtaagaac ggcaacaacc actgaccaac gcttctttac ctgatatcag gatttcttca
    12901 ttttcacctt tgcagtcacc aacaatgcag tcagcaacat caatgtgccg gacaacatca
    12961 gcggcgaagt cagaccaatg ttatccaacg cataaccgcc gattgccgca ccgcaggtgt
    13021 tagcaagctg aataacggcc acctgaatag atccagcctt ttctgcctga tcggccaatg
    13081 agcgagtgat ccacgttgac caaccaacgg gaattaaagc aaaggtcagc ccccagataa
    13141 tcgccacacc agtagcaaca attttatcgc ttccccacag cgtcagtacc agcgcgctca
    13201 gggcgagcac aaacggtgcg cccgctaagg ccagtttcac cgaacgtttg agaataaacg
    13261 acgaaagcga tgtcccgaca aagctggcga taccaaaact cagcagcacc agggttaagc
    13321 catcaacacc gaatcccgcc agattcatat acactgggcg aatataagtg aaaaaagcaa
    13381 actgaccggc aaaggacata aagatggcga tcatcccggc catcactccc ggacgttgta
    13441 ataagcggaa agtattttgt ttctgatgcg aaggttcacc tggcagcgaa ggcagtgatt
    13501 tgataatcca gaaaatatac agcacgccca tcgccgccgc cgcattaaag acattgcgcc
    13561 aaccgataag ctcgcctaaa aaactgccca acggcgcggc aatcaccagc gcaatagaaa
    13621 ccgcgccgaa gatcaccgac agcgccttcg gcaccgtacg cggcggtacc agacgcatgg
    13681 tcagcgacgc cgacatcgcc cagaacccgc ccagcgccag ccccagacag gcgcgatctg
    13741 taggctgata agacgaagcg gttttaatgc agccagtgaa cgccctcggc aggctgaaag
    13801 aacgcgttat agcgcatctg aaaagcattg atatcctgca tttcaggttc ggtttcgcgc
    13861 agaaaaccca gcgccagcct gacctgagca tcagtaatcg gcgcggcaag gcgggctttt
    13921 tgtaaaatcc gatgccattg cagcaggttc tgttcgctac catcgacaat gacaatttgc
    13981 cagatgagca acacctgcgc ggcgttttgc aggtgcgact catccgggcg atgcaaataa
    14041 cgtaaaaatt cgtcaccggc tgactttccc atttgctcga ccatcgactc cacgggtacg
    14101 acatcgatgt tcagccgatc gctgagacgg cgaatcgctt ttctggcccc ggatgtcagc
    14161 acccgaaaac caaccacaaa cactaccgcc agcgtggcga gcattatcca gatcatgagt
    14221 tctcctgcgt aatcagaact catgatatct ggaaaccctc gccagttact aatccaccag
    14281 aataatcttc agtgcaaaca gagttgcgac caccaccaca cacaggttca gatcgcgcca
    14341 gcgcccggtg cacactttca tgatgcagta cgacataaag cctagtgcaa tcccttcggt
    14401 gatcgagaaa gtaaagggca tcatcaccgt ggtaataaac gcaggcaccg attcggtaaa
    14461 atcatcccag ttaacgcgcg ccaggcttga agtcatcagc acgccaacaa agattaacgc
    14521 tccggcggtt gcgtaaggag gaaccatcgc caccagcggt gagaagaaca taactaacag
    14581 gaacataacg ccaactacga cagccgtcag ccccgtgcga ccaccgacag ccacacctga
    14641 agtactttca atataggcgg taacagacga ggtaccgata aacgcacccg ccaccgaact
    14701 gacgctatca acatacagcg ccttattcat attggggaat ttaccgttac tatctattaa
    14761 gccagcttta tcagtcacac caattaatgt ccctgatgaa tcaaataagt tgatcagcat
    14821 aaaggagaaa atgataccgg cgagttcaag tgataacgca ccgctcaaat ccacttcacc
    14881 aatgacgccg ctaatatcag gcggaatgga atagacgccg ctaaaatgaa catcaccgaa
    14941 aaacaatcca cagcaagacg tcaccacaat agaaacaagc accgcggcat gaaaatgacg
    15001 tgatgacaac acggttataa taaaaaaccc taaaatacct aacaacacgc cgtgagaact
    15061 taaatcgcca atcatcacca gggtgtcttt attggcgaca ataacgccag tattttttaa
    15121 tcccattaag gcgataaata atccgattcc gctggtgata ccaatacgta aacttaatgg
    15181 aatgttggag atcatccagt accggatacg aaacagcgtg agcaaaaata gcccaactgc
    15241 gccccagaat atcgcgccca tcccggtctg ccaggagata cccatcgccc ccacgaccac
    15301 gaaggcaaag aaggcgttca ggcccattgc cggagccagt gccacgggta agttagcaaa
    15361 tacccccatc gcaatactgc cgataccggc aatcaaacag gtggtaacaa acaccacttt
    15421 tgagtccatt tgtgccgcgc cgaggatttg cgggttcaca aaaacgatgt acaccatggt
    15481 taaaaaagtg gtcatccccg caatcaattc tgtgcggacg gtggtcccat gctgaggtag
    15541 tttaaataat cgcgaaagcg tccctgattc attactcacg taatcggtat tgtcattatt
    15601 catttttttg tccattggag gagatttaat cccttgcttt tgtaaaacaa atgacaacaa
    15661 cggttcagtg ataattatca catttcaatt gcacattaat ggatattctt taataatctc
    15721 gcgacgtttc tttatgataa ataataatca aattgataaa atcaaaatga gaaaaatatg
    15781 aataattcta ttaaccataa atttcatcac attagccgga ctgaatacca ggaattgtta
    15841 gccgtttccc gtggagacgc tgttgccgat tatattattg ataatgtctc tattctcgac
    15901 ctgatcaatg gcggagaaat ttccggccca attgtgatta aaggacgtta cattgccggt
    15961 gttggcgcag aatacgctga tgctccggct ttgcagcgga ttgatgcccg cggcgcaacg
    16021 gcggtgccag ggtttattga tgctcacctg catattgaat ccagcatgat gacgccggtc
    16081 acctttgaaa ctgctaccct gccgcgcggc ctgacgaccg ttatttgcga ccctcatgaa
    16141 atcgtcaacg tgatgggaga agccggattc gcctggtttg cccgctgtgc cgaacaggca
    16201 aggcaaaacc agtacttaca ggtcagctct tgcgtacccg ccctggaagg ctgcgatgtt
    16261 aatggtgcca gttttaccct tgaacagatg ctcgcctggc gggaccatcc gcaggttacc
    16321 ggccttgcag aaatgatgga ctaccctggc gtaattagcg ggcagaatgc gctgctcgat
    16381 aaactggacg catttcgcca cctgacactg gacggtcact gcccgggttt gggtggtaaa
    16441 gaacttaacg cctatattgc tgcgggtatt gaaaactgcc acgaaagtta tcagttggaa
    16501 gaaggacacc ggaaattgca actcggcatg tcattgatga tccgtgaagg gtccgcagcc
    16561 cgcaatctca acgcgctggc accgttgatc aacgaattta acagcccgca atgcatgctc
    16621 tgtaccgatg accgtaaccc gtgggagatc gcccatgaag gacacatcga tgccttaatt
    16681 cgccgcctga tcgaacaaca caatgtaccg ctgcatgtgg catatcgcgt agccagctgg
    16741 tcgacggcgc gccattttgg tctgaatcac ctcggcttac tggcacccgg caagcaggcc
    16801 gatatcgtcc tgttgagcga tgcgcgtaag gtcacggtgc agcaggtact ggtgaaaggc
    16861 gagccgatcg atgcacaaac cttacaggcg gaagagtcgg cgagactggc acaatccgcc
    16921 ccgccatatg gcaataccat tgatcgccag ccagtttccg ccagcgactt tgccctgcaa
    16981 tttacccccg gaaaacgcta tcgcgttatt gaggtaatcc ataacgaact gatcacgcac
    17041 tcccgctcca gcgtctacag cgaaaacggt tttgatcgcg atgatgtgtg ctttattgcc
    17101 gttcttgagc gttacggaca acgactggct ccggcctgtg gtttgctcgg cggctttggc
    17161 ctgaatgaag gtgcgctggc ggcgacggtc agccatgaca gccataatat tgtggtgatc
    17221 ggtcgtagcg cagaagagat ggcgctggcg gtcaatcagg tgattcagga tggcggcggg
    17281 ctgtgcgtgg tccgtaacgg ccaggtacaa agtcatctac cgttgcctat tgccgggcta
    17341 atgagcaccg acacggcgca gtcactggcg gaacaaattg acgccctgaa agccgccgcc
    17401 cgtgaatgcg gtccgttacc ggatgagccg tttattcaga tggcgtttct ttccctgcca
    17461 gtgatccccg cgctgaaact aaccagtcag gggctgtttg atggcgagaa gtttgccttc
    17521 actacgctgg aagtcacgga ataataaaaa aaagcccggc gtcatgccgg gcaaaagtca
    17581 ccagttacgt ttatgccact gtcaactgct gaattttttc tcgcggcgga ttttgcgttc
    17641 ttccatcacc gctactatcg ccatcaggca gatacaacca atagcggcga tatccagcgc
    17701 ggcgaaggtg cctgcccagc cggtaaggcc aaataccggc gtcccatcgg caatcattcc
    17761 cagacctaac ttggcaaagc tgtcaccaat caggtaagcg aaggtgcctt taataccatc
    17821 ggcagcgcca atcgcttttt taggtacaaa gccaacagca gccacaccaa tcaacaattg
    17881 cgggccaaag accaggaaac ccaacgcaaa gagagaagcc aggtagatat attcgttgct
    17941 ggcgtgttga tacacaccga gcgtggcgat aatcagcgcc agcgcgatac aggccaccag
    18001 gccacggcga ccgttcgcca ggtcagagag ccagccccac agcagcgtac cgaccagcgc
    18061 accagcttca aacagcgtaa agccctgaat tgccaccgct ttagagagtt tcagttcctg
    18121 gaacgcatac acggttgacc actggtcgat accaatacgc accacataga ggaaaatgtt
    18181 ggcaaagcac agcagccaga tcactttgtt tttcagcaca tactcaacaa agatctgcca
    18241 cttggtcata tcggtagatt ctgtctcttt atcctcttca ctgatctcct cgccaaacag
    18301 ctcttcagct ttgccgaggc cataagattc cggggagtcg ctgccgtaac gcagaccgat
    18361 aaaaccaaca atcagcgcga taatcgacgg gaaaataaac atgccgatga catgaccatc
    18421 gaacaggtaa tttgcgccga acagcgccac acctgctgcg cctgcaccac caaggttgtg
    18481 agaaatgttc cagaaaccga ggaatgtccc gcgtttacga cgcggcgtcc atttggtgat
    18541 ggtggagtaa ctgcacgaac cgccggtact ctggaaaaag ccgcttaagg cgtagaaggc
    18601 aatcatcagg aacaggctaa ccgagccgct gcccatactg gcactgaagc ccagcataca
    18661 aatggcagac aggatcagca tgaacggcag gaattgtttg gtgtttttgc cgtcggcgta
    18721 gtaagaaacc agcgttttac ccacgccata agtgatggag aagcccaggc cgatcatccc
    18781 cagctgcgtc atgctcaacc cgtaggtcga gatcatgtcg ttctgcgcga tgttaaagtt
    18841 cttgcgaatc aggtacatcg taaggtagcc gataaagacc accaggtaag attgcatgaa
    18901 cggtttgaac cacattttgc gccgcacttc gagcggaagg tccagggtcg gcttgcgaac
    18961 ctggtttaag aaagccagca tgggttactc ctgaaatgaa tacctgcgtg aggcatgcat
    19021 tgtaaaaatc agcagcaaat caggcctgag atagcgtcca ggcaaaacct ggaaaatttc
    19081 ttagttttgc cggatatgaa gtgaaaaggt gagatgcatc acgcttctcg cggtgtctgg
    19141 gcgttcaaaa agggcagtaa tagcagtgcg gaaatcccgg cggcgatggc gataaccaca
    19201 aaaaatccgc tccagtgcca ggtatcgagt actttcgcca gcggccaacc agcaagcgac
    19261 gcaccaagat aagcaaacaa gccgacaaat cccgtcgccg cccctgccgc ctctttgtgg
    19321 gaacactctg ccgccgccat gccgattaac atctgcgggc caaagacaaa aaaaccaatg
    19381 gtgaagaagc aggttgcctg catcacgtag ctggcaaatg gcatcagcca cagcgagccg
    19441 actgaaagca aaattccggc ggcgaaaatc aaattcatcg gcccgcggtt gccgttaaac
    19501 agtttgtccg aaccccaacc cgctaccagc gcaccgataa atccgcccag ttcaaacatt
    19561 gtcactgccg tattcgccgt gaccagatcg acgcccagca tctcggacat atacaaattg
    19621 ccccagtcgt tgatcgccgc ccgaaccaca tagaccagca catagcaaaa cgaaagcagc
    19681 cagatatacg gattcagcaa cacatatttg gtgaggatct ctttacgcgt cagccctgcc
    19741 ccttcttgtt gttgagcaat ctccagtgcg tcgtgccgcc attcaccaac cgccggtaaa
    19801 cctaacgcct gcgggcgatc gcgcagccgc cagcagagaa aaatccccac gactatcgcc
    19861 atacaacccg caatcatcat cccggcacgc cagccgtaat gcagcgcgct cgctgccatc
    19921 acaatgggaa tgagtgcgcc gccgacgtta tgcgccgtgt tccataatgc ccaccaaccg
    19981 ccgcgctcgg tacgagaata ccaggcggtt aacagacgcg cacacaccgg tgaaccccag
    20041 ccctggaaaa aggcgttcaa cacccaaagc acggcaaacg cccatagcga cgtcgagaag
    20101 ccaaacagaa tgttgataat gcccgtggca ataagcccta tccccataaa ataacgggca
    20161 tttgagcgat cgctgacaat gccggagaca aacttcgaca cgccgtaggt aatgtaaaac
    20221 agggtcgcta acaggccaat atcgctacgg ctgagcacgc cgttagcaag gatttctggt
    20281 acggcggcgt taaaactttt acgcgtgaag taaaacagcg cgtaacccag ccagatggtc
    20341 agcagaatat gccgacgcca gtagcgatag cgggcatcaa tttcatgttt atcagtcatt
    20401 aatggcgcat cggcgggcgc tttcagaaac ggcaacatca tcgaacctta gacataacgt
    20461 tgaggtagag aaacgctgac acgcgtgccg tgcagacagg aaatggtcaa tgtgccaccc
    20521 agcgccgtta cgcgctcgcg cattccggta aggccaaaac cgtgttgccc ggaatctggc
    20581 ggtaaaccgc tgccatcgtc ttcaatcacc agcatcaacc gttcatcctg ctgccagcct
    20641 tgcagggtga ccgcgctggc atcagcatgt ttcacaatgt tgttcagccc ttcctggcag
    20701 acacgaaaca gcgtcactcg ctggttttcg cttaacgctg attcatcgat tcgccattcg
    20761 agatggctga caatgccgcg cccttccagc tccatttccc gcatcagtga gcggatggcc
    20821 tgctccaggg tgagatcatc caactggcgc ggacgtaacc gacctaacaa acggcgcacc
    20881 gcgtcgtaaa cgcccagcga tagttgttcg atgagctgcc cgctctgttt cacgctggcg
    20941 ttatctgccg ccagccgctg aacaatgccc gcttgagttc gaatagcagt gatggtctga
    21001 ccgatatcat cgtgcagctc acgcgccaca tcacggcgca cgctctcttc ggtttccagc
    21061 aaccgttcag cgagatgctg gttgcgcgcc agttcctttt gcagtgactg gttaagttca
    21121 cgcaaccgct ggatgcctgc gcccagtaac aaccctgtca gactttgcac cagcagcgag
    21181 agcaataaat ccaccggatg atcgcgccag gtttggctgg cgatcagcgc gatggcgttc
    21241 atcaatgtcg caatcagcgc tccttgccaa ccatagtgcc aggccagcgc gataatcggc
    21301 agcgccagac agaatggcgt aaagcgcgac agttcgtctg gcaatcccaa ctggagccag
    21361 agactgataa caaacagcag caagtaccag accagatgtc gcccgcgcca gttgattggc
    21421 tgagaaacca gtgacgggcc gagcggcagc caggtgttat tggcgagata gtgccagaac
    21481 accaggcaaa tgggggccag cgtcaggccg ccagttaaag tcagcagcag cgcattccac
    21541 gactctttgc cgtgccaaag ccagggcagc gactgcaaca acgccgccgc cgttaacgcc
    21601 gcaccctgta acagcaaggt gcgccagtca cgttgatggc gatagcgcga gatcagcgcc
    21661 acgggcaata acgtcagtaa actaccgatc atcaataagg agaaatgggt taaaccgacc
    21721 gcctgcatta gccagtaaat cagcagccac tccgcaccca gtagcaccgg ccagtatccg
    21781 cgcgggcatt gcagcattag ccctagccgc aggccaaacg gaaataacag caccgccata
    21841 tcagggcgct caaccaggtg caggctgata ctccacaggc aaaaccatgc ggcagagaag
    21901 ataaaaaagc aggcaataac ggtaattaag cgggagaaca acgtcttcat caccagccat
    21961 caaacatgcg gcgcgccagt tcaacgtcgt tactgacgcc cagtttttcc atcagattgg
    22021 cgcgatggac gtgtaccgtt ttcggcgaca agcccagttc ggcggcaatc tctttcaccg
    22081 ccattccttg cgccagtttt tctgccacct ggcgttcgcg tttggttagc gggtcctggc
    22141 gaccggatgc cagtttaatg gcaatatccg gcgtcagata acagccgcca gtggcaaccg
    22201 tatgcaccgc agcaatgagt tcatccgggc tacagcgttt ggagagaaag ccgcgcgccc
    22261 ccgcgttaag cgcctgctca accagcgccg gactgtcatg aacggagagc ataattgtcg
    22321 ccatgccttt cggcagctgg cttagaagct ccagaccgga gatatcaggc atggagatat
    22381 cgcaaataca cacctgcaca ccgcgccccg gcagccccgc cagcgcctcg cgccccgaac
    22441 caaactcggc aactacctgc aaatcaggtt ccagccccag cagctgcgca aagccggagc
    22501 ggacgataag gtgatcgtct ataagggcaa cggtgatcat gatcttgtcc tggcgggtaa
    22561 taaaacgcgc ttaccttaac gataagcgcg atgttgttca agccttgagc ggttactgaa
    22621 aaaacaccgc aatcttgtta aacatggtcg gatcgaactg attacgctgc actttcacca
    22681 catcttccag cttgtcgatt tggcttatca tctgctccag acgctggtcg tcattgacca
    22741 gtagccagat gtggcttttg tcgctgtcct gaatcggcag gcagagaatg ccttcaacgt
    22801 taaaagcgcg acgggcaaaa aggccacaaa cgtgggtcat tacacccgga tggttgcgaa
    22861 cggtgagttc cagaatcacg ttatcatgag ttgtgttttg catggcttat tcccccacca
    22921 tttcagtatt cgccgcacct ggcggcacca tcgggtacac tttttcttcg gcatcaatgc
    22981 gcacatggat cagcgccggg ccagggcgat tgatgatttc ctgcaatgca gcctgcggat
    23041 cggcttcgtt attcaaatca caggtttcga ggccgaatcc ggcggcaatc tgcataaagt
    23101 tgattttgcc cggataggta gcggcaaaaa cgccctgctt gtagaacaga ctctgttgct
    23161 gatgcaccag ccccagcgct tcgttgttca tcaaaatgat tttaacatcc agctgatttt
    23221 cactggcggt cgccatctcc tgaatattca tcatcaggct gccgtcgccg gagaagcaca
    23281 acactttgcg gtccgggttc gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca
    23341 tcgtgcccag cccgccggag gtcagccact ggcgtgggcg attgagcgga taggcttgcg
    23401 cggtccacat ctgatgctga cccacgtcgg tggtgataat cgcattgtca tcgacacagg
    23461 cggcaacggc gttgatcagg ccgtaatggc ttaatggatc gcacgctttc gggattggac
    23521 acgggaattc acgctgcaaa tccgccacca actgatgcca ctctgcacgc ggttgcgctt
    23581 ccaccagcgg gatcaactgc gccagcacgt catcaacatc cgcctgaatt gccacatgcg
    23641 gttgcttgat tttgcccagc tctgcgcggt cgatatcgac atgaataatt ttggcattcg
    23701 gacagaactg ctcggttttg ccaatcgccc ggtcatcaaa acgcgcaccg agtacaatca
    23761 gcagatccgc ctcctgcaaa atatagttgg tgctgcgcac accgtgcatc cccagcatac
    23821 ccagcgacag cggatgcgct tttggcaaca tacccagcgc cattaaagtc atggtggtag
    23881 gcagttgcgc tttctccgcc agttcacgca ctcgcgcagg cgcgttgatc acaccaccac
    23941 ccagataaag caccgggcgt ttggcggcgt taatcatcgc cgccgcgtca cgaatacttt
    24001 cttcgctaaa ggcgggtgcg gcggcttttt ctgccatagc gggttgtgtt tcaatctcaa
    24061 aaactgccgt ttgcacatcc ttaggaatgt ctatccacac cgggccaggg cggccagatt
    24121 gcgcaatacg gaaggcatcg ctcatgacct gcgggagttc ttcgatatgt ctgaccagat
    24181 agttgtgttt ggtgatgggg atagagatgc cgtaggtgtc gacttcctgg aaggcgtcgg
    24241 tgccgatcat cgaggcggga acctgaccag tgatgcaaat cagcgggatg gagtccagcc
    24301 tcgcatcggc aatggcggtc accaggttag tcgcacccgg tccactacag gccatacaga
    24361 ccgccggttt accgtcggtg cgcgccattc cctgagcgat aaaccccgcg ccctgttcat
    24421 ggcgagccag aatatggcgg atttgcgtgc tttggcttaa ggcatcgtaa acaggcagga
    24481 tagaaccgcc cggaataccc gtcacaatct taatgccctg ctgttccagg aaatgaacga
    24541 taaattctgc gccggtaaag cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc
    24601 agtccttttc ttctgggccg actttccggg aggtgcttaa acgaaaaacc ccgcccggtt
    24661 tgcgccggcg gggttttgga atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga
    24721 ccaccaccac acgcacgacg accactgcgg cagatggcgc agttggtagt agttttgcgt
    24781 tgaacatgga agtagtcatt agggaccttg ttggttttgt gtttaacaat atttatacaa
    24841 gcacagcttt acaggggaga caatggaaaa tttttcttcc ggataaaaat acgtggcaga
    24901 tcacattttg aactgaaact caggcaataa agcatcatca cctgaaagac ggtcagattg
    24961 ttgacagagt gcgccttatt catgtcgaat acagcgtaaa caccttaccc taccaaccgc
    25021 accatcactg ttcccggcaa catttattgc cgcttttgtt tacatattct gccgctaaac
    25081 aattccccat tcctggcgta tatctggcta acattcatca atgtgataga ttcctctccc
    25141 gcatttatgg gaatgcgtag tgacttattc taattatttt tataaaagca tccgtgataa
    25201 tgaaaaggca tagaaacgtc aatttgttat tgatgttggt attactcgtg gccgtcggtc
    25261 agatggcgca aaccatttat attccagcta ttgccgatat ggcgcgcgat ctcaatgtcc
    25321 gtgaaggggc ggtgcagagc gtaatgggcg cttatctgct gacttacggt gtctcacagc
    25381 tgttttatgg cccgatttcc gaccgcgtgg gtcgccgacc ggtgatcctc gtcggaatgt
    25441 ccatttttat gctggcaacg ctggtcgcgg tcacgacctc cagtttgatg gtattgattg
    25501 ccgccagcgc gatgcagggg atgggcaccg gcgttggcgg cgtaatggcg cgtactttgc
    25561 cgcgcgattt atatgaacgg acacagttgc gccacgctaa cagcctgtta aacatgggga
    25621 ttcttgtcag tccgttgctc gcaccgctaa tcggcggtct gctggatacg atgtggaact
    25681 ggcgcgcctg ttatctcttt ttgttggtac tttgtgccgg tgtgactttc agtatggccc
    25741 gctggatgcc ggaaacgcgt ccggtcgacg caccgcgcac gcgcctgctt accagttata
    25801 aaacgctttt cggtaacagc ggttttaact gttatttgct gatgctgatt ggcggtctgg
    25861 ccggaattgc cgcctttgaa gcctgctccg gcgtgctgat gggcgcggtg ttagggctga
    25921 gcagtatgac ggtcagtatt ttgtttattc tgccgattcc ggcggcattt tttggcgcat
    25981 ggtttgccgg acgtcccaat aaacgcttct caacgttgat gtggcagtcg gttatctgtt
    26041 gcctgctggc tggcttgctg atgtggatcc ccgactggtt tggcgtgatg aatgtctgga
    26101 cgctgctcat tcccgccgcg ctgttctttt tcggtgccgg gatgctgttt ccactggcta
    26161 ccagcggcgc gatggagccg ttccccttcc tggcgggcac cgctggcgcg ctggtcggcg
    26221 gcctgcaaaa cattggttcc ggcgtgctgg cgtcgctctc tgcgatgttg ccgcaaaccg
    26281 gtcagggcag cctgggattg ttgatgacct taatgggatt gttgatcgtg ctgtgctggc
    26341 taccgctggc gacgcggatg tcgcatcagg ggcagcctgt ttaagctcac gtcaccgcag
    26401 catcgtcatc agctccatgg gagaacgatg ctgctttatc agatcacgca tcacccgcat
    26461 atgcggcgcg gagtaagaat aaaacgcctg atagcccgca caaagcacgt tgtctggttt
    26521 atcttcccga gcgtgcgggc acagccgatg ccagtcgcac gcctggcagt cacggcgcgg
    26581 ttgcgacttt tcgctgccac accatttttg taaggtggtg tcaaaaagtt caatcgaaat
    26641 acgactgata tctgcccgca cccaacactc gaacagggca attaaaaatt tcccccacag
    26701 ctctgctgtc accgaagcgg ccgtcagatc gccccggtca tcaaattcaa gatggggaat
    26761 aagctgtact gagcggatgc ccagctgttt gaaattgtga taaaaatcgg caggttgcag
    26821 agcatcctta tactctacaa ccagtttatg cctgtcctct tcagcatcaa tgacctgact
    26881 tcctgtcatg cgactctctc aataaatacg ataagttgat gtatatgcgt tacagcaaac
    26941 agtattgcga gagataacac cgcgagggag atcaaaaatt tgtcacggac tacgtgaaat
    27001 tgggaaaaat cgctattcgt gacatccatt aaattgcgat tacgggtgta gtgccagagg
    27061 atcagcgcca cgatggcgag gatgccaatc gaaatccaga ataacatgcc cgcctggtgc
    27121 cagttgtgtt tgatagccaa cgccatcaac gcgccgtagc ccagcatggt acgaaaccac
    27181 gccagtgatg tgcgttccgg ctgcagccct gggtcggcaa tgcgtctggc tttgcggcta
    27241 tccggcatac aacaccagtc ccatgacgat cactgccacg accatcaata ttaaactgat
    27301 gattaataag ctgttggtat agggcaggtc ctctttcagg cgcatcgctt tttcattgcg
    27361 taaccagcgc aagtagccat acatcgctaa cccgccggaa aacagacaca acaacaacgc
    27421 cagcagttcg cggatgacgg gcgtggcgaa atctggcgca agttgatcaa gcccaacccc
    27481 cgccgccaga aatcctagtg cagtacggat ccacgccaga aaagtacgct cattagccag
    27541 cgagaagcgg taatcgggcg cttctccgag gcgggaaatt ttcattgttg ctccttgtaa
    27601 actgttcctc gccgaaaaat taggccagga acagcaataa tgcatcagaa cttctgtttt
    27661 ttctcttcca ccttcacccc ttgtgtcggt aaaccggtgt catagtcgcg taccactgga
    27721 gaataaccat cctgtgggcg tggacgaaac gcccccatcc agcgcggttg tgcatctttg
    27781 cgccacggac gcaggttcca ttgataactg cggaacggat cgcgaatttt gtccatgtaa
    27841 tccaataagg cgtcatgcat tttgctgcga acgtcggcaa aatggatatc atcgatcagg
    27901 ttatgcattt catttgggtc attacggcga tcgtaaagtt catcactggt gaagaggttg
    27961 agcaccagtt taaagtcatc cgttacccag caacgcaccg gaataaaacc gccaaagctg
    28021 tcatgctcaa tctcgtagcg attaaattcc accaacacgc cacgtggttc ttttacggca
    28081 aggatatttt cccccggcag aatctctggt ttttcaatat ctgccagcgc catcattgtc
    28141 ggcagtaagt caatatgact gactggcgta tcgacctgtc gccgctcccc ttgcggcgaa
    28201 cggatgatca gcggaatgcg ggtgatgtcg tcatacatcg ccgctccttt actgatcagc
    28261 ttatgtgcgc ccatcatttc gccgtgatcg gaggtataaa taacccacgt attttcacgt
    28321 tgctctggcg ttaaggcatt gatgacccgt ccgatttggt catcaacaaa gtcattgcag
    28381 gcaaaataga gcggatggtg ataaagcccg tcatcaccga ctggcgatgg catcgcctgc
    28441 gcccataagc ggtgatgttc cggtttgttc gccaggtcat cctctgcttt ctcacccagt
    28501 tcgtagtaaa aatcagtgta tttctctaaa tactccaccg gacaggtgaa cgggtgatgc
    28561 ggctcatcat acgaaatcac catcaggaaa ggttcgtcgg cgcgcgcggg ctgttgcaga
    28621 aaatccaccg cccgattgct gatgcggtgc gcccaggtga aggtttcgtc aatatggttc
    28681 gcctgtaaat cctcaacgct gtttaggcca ttgcgccaca ggctaatctc tttctccgtc
    28741 agttcactaa gatagttcgc tccatcgaac cagtaatcag cgtcccactc cggcggacac
    28801 tcgccagtgc cgaaatagtc atgtccatcg agatgccatt tgccgatgta acaggtgtga
    28861 tagcccgcat ccttaaagta gcgccccatg gtggaaatgt ttttgcccgg cgcgacgttg
    28921 ttggtccacg ggccggactg gttagcgtag ataccggtga atagtccggc gcgtgcaggc
    28981 gtacaaaccg gtgaacaagt gtaggcggaa ttaaagcgaa taccttccgc cgccagacta
    29041 tcaatatttt gcgtattcag cggtttaccg ctatagcaac cgaccatatt ggtggcctgg
    29101 gtatcggtca tgatgaacag aaaattgggg cgtttcatag gctatccttc gcatcgggag
    29161 taaaggttac ggctgggtcg tgacgatgcc gccagctgtc aaaaatcagg tagacaatca
    29221 ctacggcggc gatgaaataa ctgattatcg ctaaccagcg cgtaccgtag ccaccgaatt
    29281 cagccagccc ggcatagacg ccaatcatcg cgaacaggat accaattgac gcaatcttga
    29341 cgtttttcca cggtttcatg tccaccgcaa acgcatcttt gaaggtgaac ggcgtggcgc
    29401 gcggtttgat aaaaccgatc atcagcatca cgaccacgtt gatgcagaac gtacaggcca
    29461 gcacatagag gaaatggaag tcgaacttca ccagatagtt gatggtgatg tagctgatta
    29521 tgccaatccc catcgccact tttgccgcca gcgccggaat gcgcgggaag aaaaagccca
    29581 taatgatgat ggtaaccagc ggcacgttgt aaataccgtt gagctgtttc atccagctat
    29641 acagcccctg cggcgcgttg gcgatccacg gcgcgaccat caccgaaacg atggcgataa
    29701 agaaaccaaa tttgcgcccg acggtgacca gctgttgcgg ctcgacattc tggttaatga
    29761 tgcgccggta aatacccata ctgaataacg tactggcgct attcagaaag ccgttgaagg
    29821 tactgatcac cgcaccaaat aacaccgcac cgaagaagcc caccagtggc accggcagaa
    29881 cgttattgac cagcgtcggg taggccatgt cggctttcgg taaatcctga tacagatgaa
    29941 acgcaatcaa ccctggcagc accagtacca gcgggtcgag cattttcagc accgccgtta
    30001 acagcgcccc cttttgccct tccgccaggc tttttgacgc cagcgtgcgt tgcacgatgc
    30061 cctgatttgt acaccagtaa aaggtgttca ccagaatcaa accggtaaat gccgcgccaa
    30121 tcggcaaggg atcggtcggg ccgccgactg agtttaattt ctcggcgtga acggtggtga
    30181 gttgctcaat gccctgcata aagctgccct tgcccatcgc gatcaggcca aataccggca
    30241 ccatcaaccc gccaataacc agcccaatac cgttaatgga gtccgccact gccattgcgc
    30301 gcagtccgcc gatcaccgca tacaaaattc ccgccagacc aagcaaaatc accagtaacc
    30361 agatagccgc accatcggaa agttgtagcg actcacccac atgaaacagg ctgttgagcg
    30421 ccaacgcgcc ggagtagaga acaatcggca gaaagcagac gccggtggca atgaggaagc
    30481 agaagtcgat gataatacgc gtcgttttat cataacgttc ctccagaaaa tcggggatgg
    30541 tggcaatgcc gcgcttcaga tagcgcggta aaaagattag cgcgaggaag atcagcgtca
    30601 ccgcagaagt gacttcccag cccatcaccg acatgccgct tttgtaggcc tgcccggaaa
    30661 gtccaaccag ttgttccgtg gaaaggttgg ttagcattaa cgaagcggca ataaccggcg
    30721 cttttagtga acgtccggca agaaaatagc cttgttgcga tccggtatct gttttgcgaa
    30781 ccttccacca ggtgatgatc gccaccagca gcgtaaaacc gacaaaactc aagatttgta
    30841 acgaattcat ctcatagccc ttatttatgt ttatgtcccg gacgcatgaa tatagggaga
    30901 ggtaaaatgc gtcgggtcag gcagtcagat tcttaaaata caaacgtcgt atccctgaac
    30961 ggattgtttt ctgttaagtt caggttgtga gcataattcg ccggagggat attaaaatga
    31021 atggaaaatt gcaaagttcg gatgtaaaaa acgaaactcc gtacaatatt cccttattga
    31081 ttaacgaaaa tgtgatctcc agcggaattt ctctgatatc gctctggcat acctacgccg
    31141 acgagcatta tcgggtgatc tggccgcggg acaagaagaa accgcttatc gccaactcat
    31201 gggttgcggt gtataccgta caaggatgtg ggaaaattct tttaaagaat gacgaacaaa
    31261 taactctgca tggcaactgt attatatttt taaagccaat ggatattcac tcttatcact
    31321 gtgaaggttt ggtctgggaa cagtactgga tggaatttac ccccaccagt atgatggata
    31381 ttcccgtcgg tcagcaaagc gttatttata atggcgaagt ttataatcag gaactcaccg
    31441 aagttgctga gttaattact tcaccagaag caataaaaaa taatctggca gtcgcttttc
    31501 tgacgaaaat tatttatcag tggatttgtc ttatgcacgc agtcggtaaa aaagatccac
    31561 aacggcggca aattgaaaaa ttaattgcca ctttacatgc caatctacaa caacgctgga
    31621 gcgtagctga tatggctgcc acgatcccct gtagcgaggc ctggttgcgt cgtctgtttt
    31681 tacgctatac cggcaagacg ccgaaagaat attacctcga tgcgcgtctg gatctggcgc
    31741 tatcgctatt aaagcaacaa gggaactcgg ttggcgaagt cgctgatacg ctcaacttct
    31801 tcgactcctt tcatttcagc aaagccttta agcataaatt tggttatgcg ccgtcagctg
    31861 tgctgaagaa tacggacagg taataaccag aatggcgagt gtgtaaatga ctcgatgatg
    31921 ttcagaaata ccaggcggag taccatttac ggcgactcca ccgggaaaag agttaaccga
    31981 tactccagaa gagcaccgcc agtaattggg gtgtgataat gcgcaggaac atcactaacg
    32041 gatagacagt ggcgtaagag agcgccgccg caccactggt tggatgaaga ttattagcaa
    32101 acgccagcgc cggaggatcg gtcatggaac ctgccagcat cccgcacatg gtcaggtaat
    32161 tcattttggc taacatccgc gccagaatgc caacagtaat cagcggaacg gcggtgatca
    32221 gggcaccata accaatccag cttagccctt cgccatccac cagtgtatgg ataaaatcgc
    32281 cgccggattt caggccaacc accgagagaa agagcacaat gcctaactcg cgtagcgcca
    32341 ggttagcgct tggtggcata aaccagtaca gcttgccgat actgccgata cgcccgagga
    32401 tcaacgccat aatcagcgga ccgcccgcca gcccgagttt caacgccgcc gggaacccag
    32461 gcacaaagac gggaatagaa cctaacaata cgccaagccc gataccaata aacactggca
    32521 gcatctgaac ctgttgcagt ttttgctgcg cattccccag cacattggca acggcatcaa
    32581 ttgcggacgg acgccccacc aggttgagga tatcgccgaa ctgcaggctg atatcgctac
    32641 ttgcaactaa ttcaacacca gcacggttca ggcgcgagat aacaacgtca tagcgttctt
    32701 taaagtgcag gtcgcgaata cgtttaccga gcacgttttc attggtgacc accacacgcg
    32761 cgacgcgcaa atcagtgcct ttcgtagaca gcgaggtatc gacctcctga ccaatcacca
    32821 gttgcgcatt atgtaaatcc gcaggctgcc ccaccagatg cagcaaatcg cccagttgaa
    32881 taacggtttc tggcgaaggc acttttaggg tttcttctcg tttcaatcgt gagcaaataa
    32941 ctttatcgcc attgagaatc ggcacatctt taatggctaa attatgcaaa ttaggattct
    33001 caactcggat gttaatcgtc ctgattagcg caccgccgtt ggttcgcgtg gactcgtgct
    33061 gctgggcctc agtctcaaca ttgacgcgga aaataacccg cagcatccac atagtgaaca
    33121 aaatgccgca aatcccaaat ggatacgcca tcgcatagct catccccatt tgatcgacca
    33181 tcgccatcgg tgtccccagg tcgcgcaaga tctgctgccc tgcccccagc gctggcgtat
    33241 tggtgaccgc gccggaaaaa atccccagca ctaccggcag tggaatatca aacagtttat
    33301 gcaggatggc ggtaaccaga ccaccgatga tgacgatcag aacagcaaac aggttgaggc
    33361 gtaatccgga gacgcgcaat gaggcaaaga agcccggccc tacctgaatc ccgatggtat
    33421 aaacgaacag gatcaggcca aactcctgaa taacatgcag catatcgcta cttaatgtca
    33481 tccccgcctg agaaacaaaa tggccgacga tgatcccacc gaacagcacg ccgccaatgc
    33541 ccaatccaac accgcgaaac ttgacgttgc cgataaacaa cccgacgacc gccaccaaag
    33601 ccagaatact gaccgttaat gctatatcac tcatcgtcca tttctcttga aataacattt
    33661 taattgctac ggattatggc tgaatcggtg aagcagattt gccttatagc gcacattatc
    33721 acgggaaata acgtgccgaa atatcttaaa caggcgaaat ttaacgcatt gctgaagaaa
    33781 aaagacaaaa aaaaagcccc accagaatgg cggggcaaag agaatagcta gttagctatt
    33841 taacgcggga cgttcgctga tagcgatacg ctgcgctgcg atgggttcag gctcattacg
    33901 aattaaatca atatgcagta aaccgtttac gaaggttgcg ccagagactt ccatattttc
    33961 agccagcgta aagctcagac taaaaggctg attcataagc ccttgatgca gccatttttt
    34021 ctcttctttt ggctgctccg gcgtgccttt tacgctcagg cgcgtacctt ccagttgaat
    34081 ctctaaatct tcctgacgga aacctgccag cgcaagggta atgcggtagt ggttatcgtc
    34141 gcttttctca atgttgtacg gcgggaagct ctggctttca ccggcgtttt gcagtgcgtt
    34201 ggccagtttg tcaaaaccga tccattgacg catcagtggg gataaatcga agttacgcat
    34261 agtcatttct ccttctaaga agcgagtaag tacctgcaaa tccgaagatt cgcatatgct
    34321 ccctgacggc gagcatggaa atgtcaggcc gcgccaggcg gccttaggga attagttgat
    34381 ttcgatacgg cgcggttttt tcgcttccgg aatcacgcgt tcgagatcga tatacagcag
    34441 accattcacc aggttagcac cacgaacatg aatgttctca gctaactgga atttgcgttc
    34501 aaagttgcgt tcagcgatgc cctggtacag ataggtgcgc tctttttgtt cgtcggcatg
    34561 agcacctttc accaccagca gattatcctg ggcggtaatt tccagttcgc tctcagcaaa
    34621 accagccaca gcgatagcaa tgcggtaatg gttttcgtct accagttcaa cgttatacgg
    34681 agggtagccg ccattactct ggctctggtt gttttctaag tggttaaaca aacggtcaaa
    34741 tccaatagca gaacggtaaa gcggggataa atcaaagtta cgcataatca atagctcctg
    34801 aaatcagcga gaatgtaaga ccttccacaa tggacaggtc aggtagccag aacacccatc
    34861 aggcgcgtcc tcatcggcta cgatgtaaaa atgggtctgg aaatgaactt ttcaagcctt
    34921 atcagcggac ttttttttgc agtttatggt ctattgcata gactgagggg gcagcacgaa
    34981 acgttaaagt gcgatagccg ccacagtgcg acgtaatgat gatgttattt tcagcaaggg
    35041 atcgctataa ctcatcaggc caacacccac gatcaacaga tgaatttatt atgataagaa
    35101 atgtgttgtt agcgttcatg atatgcagcg gaatgacatt actcggagga tgctccagcg
    35161 tgatgtccca taccgggggg aaagaaggaa cgtatcctgg cacccgcgcc agcgcaacca
    35221 tgattggcga cgatgaaacc aactggggca caaaatcgct ggcgatactc gacatgccat
    35281 ttaccgcagt actggacaca atattgctac cgtgggatgt gttccgcaaa gacagctcag
    35341 tgcgctcgcg cgttgaaaaa agcgaagcca acgcacaagc caccaatgcg gttattccac
    35401 cggccagaat gcctgataac tgatttagcg atcagtttcg gtgatccaca actgaccgtc
    35461 ctccatccac gccaaccatt gaccatccgg cgagaaaacc acggcgtccg cagaaggtgg
    35521 attggcgtgg ttttcggtta aatactcaac ctcgccgctt tgcgcatggg cacaagcaat
    35581 ccgattatcc agcacaaatc ccagccattc gcctgatgga tgccagttaa atgcagactg
    35641 aatatccgtt ttattatggg ttaactggcg tggttcaccg ccctgtggcg agataagcca
    35701 cagttgcaca atgccgttat catcacgcat taaaaacgcg atttgcgtac cctgcgggtt
    35761 acagcgcacc cagtggcgcg ggacgttgac taaccccgga taagcccgat ggtgggtaaa
    35821 ggttaaacgt cgctgcacga cgccacgcgg tggcgcgggc agggttgttt ccgttccact
    35881 taacggcgca tcaccagctg ctttccagcc agcttcatct ttcggcaact cgacaataaa
    35941 cagctccggc actttctcgc cctttggcga aagtgtgtcg ccaataaacg ccagtgcgtg
    36001 atttcctacc catccttctt cataagcacg attgatttca ttactgccag gctgcggcgt
    36061 gggcgttgtt ttactcacca gtacgcacca gtggctaccg ctgtattcac gcggatgctg
    36121 cttttgtacg ttgaccgggc caaacggtgc agcaacaccg acatttcgca aatccagcgc
    36181 cggatcgagt tcatgcatta catggtcgtt gtaggtaaag ctcacccttt caccgtttgg
    36241 gctaaagaca tgtacatgac tgccgccgcg cagcgctcct ggagtatatg acgcggtaat
    36301 atccattgca tcgagattgc tcaccttgcc gctttcaaca atcactccgc gccgatgatg
    36361 aaagtcgtaa taccatgttt catcaggatt ttccgggccg tggatgaaga catacttctc
    36421 tgacttcgga tgaacggtca ccacgccgac gtatgcgccc tgtgacgcgc gatagataac
    36481 ctcgacctcg ccggtatgaa tattcacacg ctcaatggtc tcgccggtaa acgacgcccc
    36541 agaaggccgc acgtcaaaca ccagccactg gctgtcgggc gtccaggtat tggtattggt
    36601 gagcaggtga ttacggggag caaaggttat ctgtttcatg gcgcggtcct gatacaaaac
    36661 ggttcccgcc atcatacttc acaaggactt cactttcaga caatagtctt ttgccatcgt
    36721 gatgaatgtg agtgaaaaga tggaacattt cgacgtggca attattggcc tcggcccggc
    36781 agggtcggcg ttggcgcgaa agttagcagg caaaatgcag gtgatcgcgc tggataaaaa
    36841 gcaccagcat ggtactgaag gtttcagcaa accttgtggc ggtctgctgg caccggacgc
    36901 gcagcgatct tttattcgcg atggactgac gcttcctgtc gatgtcatcg ccaacccgca
    36961 gattttcagc gtcaaaaccg tcgacgtcgc cgcatcgctc acgcgtaact accagcgaag
    37021 ctatatcaat attaatcgcc atgctttcga cttgtggatg aaatcgctga tccccgccag
    37081 cgttgaggtt taccacgaca gcctgtgtcg aaaaatctgg cgtgaggatg ataaatggca
    37141 tgtcattttt cgcgcagacg gctgggagca gcatattacc gcccgctatc tggtcggtgc
    37201 cgatggtgcc aactcgatgg tgcggcggca tctctacccg gatcatcaga ttcgtaaata
    37261 tgtcgccatc cagcagtggt tcgcagagaa acatccggtg ccgttctact cctgcatctt
    37321 tgataatgcg ataactgact gttactcatg gagtatcagc aaagacggtt attttatctt
    37381 tggcggtgcc tatccgatga aagacggtca gacgcgtttc acgacgctaa aagagaaaat
    37441 gagtgccttt cagttccagt ttggtaaggc ggtgaaaagc gaaaaatgca cggtgctgtt
    37501 tccctcacgc tggcaggatt ttgtctgcgg caaggacaac gcctttctga ttggcgaagc
    37561 ggcgggattt atcagcgcca gctcgctgga ggggattagc tatgcgctgg atagcgcaga
    37621 gattctgcgt tcggtattac tgaagcagcc agagaagctc aataccgctt acaggcgcgc
    37681 cactcgcaaa ctgcgtttaa aactcttcgg caagatagta aaaagccgat gcctgaccgc
    37741 accggcttta agaaagtgga ttatgcgcag tggagtggcg catattccac agttgaaaga
    37801 ttatccaacg cgcttcacat cgcccaccag caggatgtaa gagagcgcgc caatcaacgc
    37861 gacggcggag atataaacca gtgcaggcgc gaaaccgtaa ccctgcgcca ggtatcccac
    37921 caccagcgga acggtgatgc cgcccagacc accggcaaag ttaaacacgc cgccggttaa
    37981 accaatcagg cgcatcggtg ccagagaaga gaccagcgac caggttatcg aagcaaaacc
    38041 gttgccgaag aatgccagcg ccatcaggca cataatcatc atcggatcgt tggtgtagtt
    38101 cgcacccata atgcaggtgg agatcagcaa gccgcagata atcggcgttt tacgcgcaaa
    38161 gcccagtgaa aagcccttac gtaccagcag atccgcgacc cagccagaga gcaggacgcc
    38221 aataaacgcc gcgaggaacg gcaccgtggt cataaagcct gctttcagcg ctgtgattcc
    38281 tttttcctgg gttaaatagt tcgggaacca ggttaagaaa aaccacagtg tagaagccac
    38341 cgcaaattgc ccaagataga caccgatcag tttacgatgg aacaccagtt tccagtcttt
    38401 ggctgttaac ggctgacgcg cctctttctt caccggcgca tcgccatcca ccagaccgcc
    38461 gccatcacga atgtaatcca gttcagcttt gctgatgcct ttggtcagac gcggcggctg
    38521 ataaacctta aaccaaatca gcgaccagat aatgccgata ccaccggtga caatgaacac
    38581 ccagtgccag ctcaacatct cctgaatcca gatcagcagc ggagtcagaa acgccagacc
    38641 gacaaactga ccagacgtat aaaaaccaac ggcagaagcg cgttcatgtt ccgggaacca
    38701 actggttacc atccggttat tggtcgggaa cgcgggcgct tcgaaaatac cggttatcgc
    38761 gcgcagacca attaatgaca ttaagcccgt ggcaaagccc tggaataacg tcgccactga
    38821 ccagccaaat atcgcaataa aataagtcac gcgtgaacct acgcgatcta aaaaccaacc
    38881 accggggatc tgacatagcg tataaagcca ggcgaaggcc gaaaatacat agcccatttg
    38941 cgctttagta ataccaaact cttcctgaat atgggcagaa gccacggcca ggttagcgcg
    39001 gtcaacataa caaatgacca ccgtaataaa gatcatcacc agcgtcagat aacgccgacg
    39061 ccccggcttt gctgcattaa cgggaatatc catagcgagc tttctccaga ttttgggcat
    39121 agcgaagccg ctcaccatgc cctgtaattt acagagggtt atatttttgt attgctgttt
    39181 tagtgcccga tgaggggctt acgtggcagg aattaccact ctgctacgct gttatcttca
    39241 tgacgccaga gcggattacg ccagtccggg gcatttttac tgaactcaat aactttagct
    39301 tcgtcgattt ccacgcctaa gcccggtttc gttaatggtt taaagaaacc gccgaccatg
    39361 ctgaagtcct ctttgttttt cacaaagtcg agtaactccg cgcctttgtt gtaatgaatg
    39421 cccatacttt gttcctgaag gacggcgtta taggaaacaa agtcgatatg caggcaagcc
    39481 gccagtgcaa tcggtccgag cggacagtgc ggcgcaaggg tcacgtcata ggcttctgcc
    39541 attccggcaa ttttgtagca ttcggtaata ccgcctgcgt gggagagatc cggttgcaga
    39601 atcgaaatac cgcctgcctc cagcacacgt ttaaaatcga agcgtgagaa catgcgctca
    39661 cccgccgcca gtggaatatg cgtttgtgcc gccagtttcg ggtagtattc ggcctgttcc
    39721 gccagcaccg gctcttcaat aaacagcgga cgatacggtt ccagctcttt aatcagcact
    39781 ttcgccatcg gcgcgctgac gcgaccatgg aaatcaagac caaactcaat ctgattgcca
    39841 aaagcttcac gaatttgtgc cacggtgttg actgccgcat ctaccgcgcg ggagttatca
    39901 attagcccca gttcttcaca accgttcagt ttgaaggtat cgaagccgat ttcgcgtagc
    39961 gttttaatgc cgtcgataac atccgccgga cgatcgccgc cgacccaact gtaggcttta
    40021 attttgtcgc gaaccaggcc gcccatcagt tgccagaccg gcgcattcag cactttacct
    40081 ttgatatccc ataacgcctg gtcgatcccg gcgatggcgc tcatcaggat tggaccgcca
    40141 cgataaaatc cggcgcgata catcacttgc cataagtcgt tgatgcgtga cgggtcctga
    40201 ccaatcaaat agtcactcag ctcgtggact gccgcttcca ccgtgcgagc gcggccttca
    40261 atcacgggct cgccccaacc gaccacacct tcatcggttt caattttcag gaacatccag
    40321 cggggaggta accgatacgt ggtaattttg gtgattttca ttgcactgcc tctcgatacg
    40381 ccttaacaaa tgctgctgcc tgctgcgcag tccgttctac ggactgcccg gcgcgataga
    40441 gatcgctgcc taagcccgcc cctgcacaac ctgcgtctat ccactgcgcc aggttttctg
    40501 gcgtcacgcc gccaacggca aagactgcga tgtccggtgg taataccgct tttaatgctt
    40561 tgatgtattg cggaccaaaa gccgatgacg gaaatatttt cagcgcctgc gcgcccgctt
    40621 cgagcgcggt aaaggcttcg gtcgccgtcg cacagccggg gcagacggtc atgccgtagc
    40681 ccaccgcacg gcggatcact tcactatgga tattgggcgt aacgatgagc tgacagccca
    40741 ttctggcgag cgcatcgacc tgttcaggtt tcagtaccgt acctgcgcca atcaacgcct
    40801 tatcgccgta cgcatcaacg atggcgggaa tgctctgctc ccattgtgga gaattcagcg
    40861 ggatttcaac cgcatcgaac ccggcgtcaa tcaccgcgcc aacatgtgcc agcgcctcgt
    40921 cgggcgtaat gccgcgcaaa atggcgatca gcgggagttt agtttgccac tgcatgagcg
    40981 atgctcctta taccagcctg aaatgccgtg tcgcctgcca ccgccgtcac gtcgcaaccc
    41041 atcgcctgaa aggcttgctg gtagcgcgcg gtcagcgatg ttccggcgac aagggtgatg
    41101 gcgtgttgat gggccacata gtcacgcata ctggcaacct ctgcgccaat caacaaacca
    41161 gagagaaatt cgctgacctg ttcgcgggga agtgttccca gcacatgcga ggcgcgaacc
    41221 tcaaaaagct gtggcaatac ggcgggcgca ttaaggccac gctcaagacc agccgcgaag
    41281 gcatcggcag cgttttcctg cggcggcaaa cctgcgccaa tcaatgagtg atttaacagt
    41341 aaatgatgta attcaccgct catcacggtg cgaaaatcgt taatttgctg gctatcggcc
    41401 tgcacccatt tgcaatgcgt accgggcatg acataaagag aggaaggagc gagagtgcgc
    41461 gcgccgatca attgtgtttc ttcgccgcgc atcacattgt ggttatcgtc atgagagacg
    41521 cataatccgg gaataatcca gatattgtcg ccaacagacg ttaattgttc gccaatagac
    41581 gaaaaacggg ctggaacgga taaatacggc gcaactttcc agccgacatt gctgccgacc
    41641 attcctgcca ttaccactgg cgtattctct tcacgccagt cggtcgtcac ttctgctaac
    41701 accgcagccg gagattttcc gttcaggcgc gtgacgcctg cttctgattg cctgcagtcc
    41761 aggcagtggt cgccctgata aagccaggcg cgcagattgg tcgatcccca gtcaattgcg
    41821 atgtagcgag ctgtcatgtg atttccttta accttcgtgt cgagctggcg atcatggtaa
    41881 gcgccgcctg ctctgccgca tcgccgtcct gatgccttat cgcatcgaac agcgccttat
    41941 gttcctggag cgtttgcggc atgttggcct catcgcccat ccaggttcgt tcaaaaaccg
    42001 cccgctgcag cgaactgatc gcaatgctca gttgctgtaa caccgggtta tgtaccgact
    42061 gcagcaccgc ctcgtggtag cgaatatccg cttcgttaaa cgcttcgcgg tcctgattgt
    42121 tagcaatcat ctcgttcagc gccgattcaa tctgcgccag atcgctggag gtcgcgcgct
    42181 ctgctgccca acgggcaatc gccggctcta ccaggtttcg cacttcgctc attgcactga
    42241 taagccgtgg gtcatagtcg ttttccagca cccattgcag tacgtcagtg tcgaggtaat
    42301 tccactggtt acgcggagcc acaaacgccc cgcgataacg tttcatttca atcagccgct
    42361 tcgccatcag cgaacgaaat acctcacgga tgatgttgcg cgaggttgca aactcctcac
    42421 agagttctgc ctcagccgga agcggcgagc ctggcacgta tttgccgtga acgatctgtt
    42481 tacccagcgt aataacgatg cgatcggttt tattgagagt catggagagt ccttgtgctc
    42541 tgtatgttct tctctacttt accccgatcg atgcataacg cggcaatttt gtagtaccag
    42601 cgtgatgacg ttcgcgtttg ctgtgcgcct aatgtagtac aaagttatat tgttgtacta
    42661 caatttagat cacaaaaaga acaatccata aaaaaatgtt ctacgtcggg caaaaatctg
    42721 aaaagggaga tcaggcgcta aactgggggg aaagaagagt atgctttcaa cggcttagta
    42781 ctcttgttta aaggattaat catgaagttg aatcttaagg gatttttcaa ggctgccagt
    42841 ttgtgttctt tggcatttgc gttgaccgga tgtatcacct ggggattagt ttccaatact
    42901 gcaagccatc cacatgccga gcaatggcgg tcagacacga ttaagggttt atcgctggca
    42961 gaagatagta acggtacgaa aggctatgtt tttgttggtg aatcgttgga ctatctgttg
    43021 accaccggcg gcgatgaggt ggttaacatg ctgaacgatc cggcaatcca tggtgaaaga
    43081 atcaccgttt ctgataacgc gaaatttatt ttaagcagca gtaataagaa tttctctggt
    43141 gctattacgc tctattacga ctggaacaac gaagaagata aggcactggc aacacaatat
    43201 ggttttattt gtgatacccg gcgttgtaca tggatgctgg acggtttgaa aggctcaatc
    43261 caccagaaaa ataaaaaagc ggattactca aacgtgatgg ttttccatca accctttacg
    43321 gtgggatttt atgagtacaa agcaacagat ggcgtcccgc gcggcctcgt taatgcgctg
    43381 ctgccagtca ctcttacgct ggatatagta acgtcaccac tccagttcct gattttatgc
    43441 acgaccagaa attgttaatc gctgcgggca aacgcattca tgccgtttgc ccgttcactg
    43501 attaattcag cacatacttc tcaatagcaa atgccacgcc atcttcaagg ttggatttgg
    43561 tgacaaagtt cgccacttct ttcaccgaag gaatagcgtt atccatcgcc acaccgacgc
    43621 ctgcatattc aatcattgcg atgtcgtttt cctgatcgcc aatcgccatg atttcttccg
    43681 gtttaatacc taacacgtcg gccagtgatt tcacacccgt acctttgtta acgcgtttat
    43741 cgaggatttc gaggaagtac ggcgcacttt tcagcacggt gtatttttct ttcacttcct
    43801 gcggaatacg cgcaatggcc tggtcgagga tggcgggttc atcaatcatc atcactttca
    43861 ggaactgggt attggggtcc attttctccg cttcgcagaa caccagcgga atggtggcaa
    43921 cgaaagattc atgcaccgtg tagtagctga tatcacgatt ggcggtgtac agcgtggtgc
    43981 ggtccagcgc gtggaaatga gaaccgactt cgcgagagag tttttccagg aaacggtagt
    44041 cgtcatagct gagagcggtt tgcgccacgg tgctaccatc agcggccttc tgtaccagcg
    44101 cgccgttata agtaatgcag tagtcgcccg gctgttccat atgcagctct ttcaggtaat
    44161 tgtgaacacc tgcataaggg cgacccgtcg ttagcacgac attcacgcca cgggcgcgag
    44221 ctgcggcaat cgcattttta acggcgggtg aaatggtgtg atcgggcagc agaagggtgc
    44281 catccatatc gatagcaatg agtttgatag ccatgagttc cccaggtaga ttggttcctg
    44341 acccatgcta acgcgattcc gctcaaaaat cagtacaaca cccgagggaa aagggggatg
    44401 caacgcgcat tgcgtgctcc ccatttgctt agcggaaaag tttccctttc agcagttcca
    44461 tgcctgcgga aagcagatcg ttattggctt gtggtgacac ttcaccttgc ggtgagagcg
    44521 catcaataat cttcggcaat tgttctgcca gtaaactgga agctgtactg gtatccacgc
    44581 caagtttttg cccgagatcg aacaccgcat ttgtgccgag cgccgattcc acttgctcgc
    44641 cgctaactga ttgattgctc tgttgattac tcagccaggt tgagagaatg gcccctaagc
    44701 cgccactttg cagtttttcc aacagcacct gaatgccgcc ctgttcctca acccagctca
    44761 aaatggcctg atatttcccc gcatcgcctt tcagaaaggc accgacaacg tcatcaaaaa
    44821 gccccatgat aatcacctgt aaagcgttac gtgttgaccc aaaaagtata gatctgcggg
    44881 atgataattg cggattgcag aaataaaaag ggcggagatg atctccgccc ttttcttata
    44941 gcttcttgcc ggatgcggcg tgaacgcctt atccggccta caaaaacatg caaattcaat
    45001 atattgcagg agtctcgtag gcctgataag cgtagcgcat caggcacgct cgcattgtta
    45061 acgccattaa atatcgatat tcgccgcttt cagagcgttc tcttcaataa acgcacggcg
    45121 cggttcaacg gcgtcgccca taagcgtagt gaacaactgg tcggcagcaa tcgcatcttt
    45181 aacggtaacg cgcagcatac gacggctttc cgggtccatg gtggtttccc acagctgttc
    45241 cgggttcatc tcgcccagac ctttataacg ctggatggag aggccgcgac gggactcttt
    45301 caccagccag tccagcgcct gctcgaagct ggctaccggt tgacgacgtt cgccacgttc
    45361 gataaatgca tcttcttcca gcaagccacg cagtttctca cccagcgtgc agatacgacg
    45421 atattcgccg ccagtgataa attcgtgatc cagcggataa tcagtatcca caccgtgggt
    45481 acgcacgcga acaatcggct cgaacaagtt ttgctcagca ttggtgtgga catcaaactt
    45541 ccactggctg ccgtgctgtt ctttatcgtt cagttcgctg accagcgcgt tcacccagcg
    45601 ggtaacggtc tgctcgtcag aaaggtcggt ttccgtcagc gtcggctgat agataagctc
    45661 tttcagcatt gctttcggat aacgacgctc catgcggttg atcattttct gcgtcgcgtt
    45721 gtactcagac accagtttct ctaacgcttc gccagccagc gccggtgcac tggcgttggt
    45781 gtgcagcgtt gcgccatcca gcgcgataga gatctggtac tgatccatcg cttcgtcgtc
    45841 tttaatgtac tgttcctgct tacctttctt cactttgtac agcggcggct gagcgatgta
    45901 gacgtgaccg cgttcaacga tttccggcat ctgacgatag aagaaggtca acagcagcgt
    45961 acgaatgtgc gagccgtcga cgtccgcatc ggtcatgatg atgatgctgt gataacgcag
    46021 tttgtccggg ttgtactcgt cacgaccgat accacaacca agcgcggtga taagcgtcgc
    46081 cacttcctga gaagagagca tcttatcgaa gcgcgctttc tcgacgttga ggattttacc
    46141 cttcagcggc agaatcgcct ggttcttgcg gttacgcccc tgcttcgcag agccgcccgc
    46201 ggagtcccct tccacaaggt acagttcgga aagcgccgga tcgcgttcct ggcagtctgc
    46261 cagtttgccc ggcaggccag ctaaatccag cgcaccttta cggcgggtca tttcacgcgc
    46321 gcgacgcgca gcttcacggg cacgggcagc atcgataatt ttgccgacca cgattttcgc
    46381 gtcggttggg ttttccagca ggtattccgc cagcagttcg ttcatctgct gttcaaccgc
    46441 cgatttcacc tcagaagaaa ccagtttgtc tttggtctgt gaggagaatt tcgggtccgg
    46501 cactttcacg gaaacgaccg caatcaggcc ttcacgcgca tcgtcaccgg tggcgctgac
    46561 tttggctttt ttgctgtagc cttctttgtc catgtaggcg ttcagggtgc gggtcatcgc
    46621 cgcacggaag cctgccaggt gagtaccgcc gtcacgctgc ggaatgttgt tggtaaagca
    46681 gtagatgttt tcctggaagc catcgttcca ctgcaacgcc acttcgacgc caataccgtc
    46741 tttttcggtg gagaagtaga agatattcgg gtggatcggc gttttgttct tgttcagata
    46801 ttcaacgaat gccttgatgc cgccttcata gtggaagtgg tcttctttgc catcgcgctt
    46861 gtcgcgcaga cgaatggaaa cgccggagtt gaggaacgac aactcacgca gacgtttcgc
    46921 cagaatgtca tattcgaact cggtcacatt ggtgaaggtt tcaaggcttg gccagaaacg
    46981 caccatagtg ccggtttttt cagtctcgcc ggtaaccgcc agcggggcct gcggtacacc
    47041 gtgttcgtag atctgacggt gaattttacc ctcgcgctgg ataaccagct ccagtttttg
    47101 cgacagggcg tttactaccg aaacaccaac gccgtgcaga ccgccggaca ctttatagga
    47161 gttatcgtca aatttaccgc ctgcgtgcag aacggtcatg atcacttccg ccgccgatac
    47221 gccctcttcc gggtgaatac cggtcggaat gccgcgcccg tcatcctgta cagagacaga
    47281 gttatcggcg tgaatggtga cgataatttc tttacagtga cccgcgagcg cttcgtcgat
    47341 agcgttatct accacctcga ataccatgtg gtgcagaccg gtgccgtcat ccgtgtcgcc
    47401 gatatacata cccgggcgct tacgcaccgc atccagccct ttcaggactt tgatactgga
    47461 ggagtcataa gaattcgaca tcaacgtttc tcgctcattt atacttgggt taatccgtta
    47521 ttttaccctt ttccacggta aacatcttcg aattttcgtc cgacatgtct ataacgtgtt
    47581 cagcactgat cgcgctgaca aagacctgtg attgcgtcgc ttttaagcgg ctggcaagca
    47641 gcccgcgacg ctcatcatca agctcagagg caaaatcatc tatcaggtag agacaccgcc
    47701 gcccgctttc acgggtgagg aactctcctt gcgccagacg taaggcgcac atcaacagct
    47761 taagctgccc acgcgataag gtatcttcca ccggcgcacc gtcggcgcga atgcgtaagt
    47821 ccgctttatg cgggccgtgc gcggtgtagg ttagctggcg atcgcgttca aaattacgtt
    47881 ccagcacctc agcgtactct atctctttct cccagccgcg ctggaaagag aaagtcagag
    47941 aaaactcagg gagaaattgc ttacaggtat cagccatatc ggctgcgata ccggcgctat
    48001 actccgcgcg ccaggtgctg atttgctccg cctgcgggat cagttcttta tcccacgggc
    48061 gtagctgttc gtaacgagtc acctggcgca gcgccgcatt gcgctgcttg agcaatcgct
    48121 tgagattgct ccaggcggtg aaaaatccgg gttcgttgtg aaagcatccc cagtcgagga
    48181 atgctcttct gtatttgggg ccgccgttga gtaaagtaaa cccttctggc gttatcagct
    48241 gcattggcat caggtgcgcc agctccgcga ctttatgccc gtctgtaccg tcaatacgga
    48301 ctttgctgtc gccttgtttg tctttggtta aaccaatcgc cgtctcgcgc tcttcgccct
    48361 gtaatcgccc gtggagaaca aacgcctcct gctcgtggcg aatgacgcga ccaatctgca
    48421 aactgcgaaa cgcccgaccg tggccgagcg tatagatggc ttccagcacg ctggttttgc
    48481 cactgccgtt ggcacctacc agaaagttaa agccgggaga taaggcgaga tccgcggttt
    48541 caatgttgcg gaaatcgcgg atcaacaagc gggtgaggga cattacagtc tcattggcat
    48601 gacaacataa gccgcgctct ggctggccgc atcttcaatc tgcacgctgg aaaccgaatc
    48661 ggtcagcatc atgcggacgt tttcgcattt cagtgcgttc agaacatcca gcacatagct
    48721 gacgttgaag ccgatttcca tctccgcacc gctataggta acgtcgagga tctcttccgc
    48781 ttcttcctgt tctgggttgt tggcggtgat tttcagctgg ttttcgctga catacagacg
    48841 cacgccgcgg aatttctcgt tagagagaat tgccgcacga gcaaacgcct gcttgagcag
    48901 atcacagcca gcttccagat gtttgtccgg gttcttcggc agaacgcgac ggtaatccgg
    48961 gaagcgacca tccaccagtt tcgaggtgaa gataaagtcg ccaacgtggg cgcgaatgtt
    49021 gttgctgcca atctgcacgc gcagcggatt gtcgccgccg tcgagcatac gcatcagttc
    49081 aatcacgcct ttacgcggca cgatcaccga atggcttggc aaagattgac caattggcat
    49141 tgaacagact gccagacggt ggccgtcggt cgccacggtg cgcagttctt caccttcggt
    49201 ttcaaacagc ataccgttta agtaatagcg aacgtcctga tgcgccatag aaaactgggt
    49261 cgcttcaatc agacgcttca tcgtcgcctg cggcagggta aattcgactt cgctctgcca
    49321 gtcatcgagg ttcgggaaat ctgccgctgg cagggtagac agcgaaaaac ggctacgccc
    49381 ggagcgcacc agcatccgct caccttccag ctgcacggca atttccgcgc cttcaggcag
    49441 accacggcag atatcaaaga atttgcgcgc cggaacggtc gtcgcccctg gttcgtgcgg
    49501 ctgaaccagc gcaacgcgtg ccaccatttc catctcgaga tcagtaccgg tcagcgacaa
    49561 cgtaccgtca gcaacctgta acagcagatt accgagaatc ggtagcgtag gacgaccacc
    49621 taacggaccg ctcacctgtt gtagcggttt taataaatgc tcacgttcta cggtaaattt
    49681 cataggttta cgatgacaat gttctgatta aattagaaaa atcttctttg atatcgtggc
    49741 tctcttcacg caactgctcg atcttccggc aggcatgaag caccgtcgtg tggtcacgac
    49801 caccaaaggc atcgccaatc tccggcagac tgtggttagt cagctctttc gccagcgcca
    49861 tcgccatctg gcgcggacgg gccaccgagc gggatcgacg cttggaaagg agatccgcga
    49921 ctttgatctt gtagtactcc gccaccgtct tctgaatatt gtcgatggtg accagttttt
    49981 cctgcaatgc cagcaagtcg cgcagcgcct cacgcacgaa gtcgatggtg atcgctcgtc
    50041 cggtaaagtt ggcgttggca atgacgcggt tcaacgcgcc ttccaactca cgtacgttag
    50101 atcgtagacg cttggcgata aagaacgcca cttcgcccgg caaacgaatg tcgttttcgt
    50161 cggccttttt catcaggatc gccacgcggg tttccagctc tggcggttcg atcgccacag
    50221 tcagtcccca gccgaagcgg gatttcaaac gatcctcaac gccgttgatc tctttcggat
    50281 agcgatcgga ggtgagaatg atctgttgat taccttccag cagggcgttg aaggtgtgga
    50341 aaaactcttc ctgagatcgt tctttattag caaaaaactg aatatcgtcg atcagcagtg
    50401 catctacgga acggtagtag cgtttaaact cttcgatcgc gttgttttgc agagctttaa
    50461 ccatgtcctg aacaaagcgc tcggagtgca tataaaccac tttggcattc ggcttgcgcg
    50521 ccataatgcc gttacccacc gcatgcagca ggtgagtttt acccaaaccc gtgccgccat
    50581 aaaggaacaa cgggttataa gcaccgccag ggttatctgc cacctggcga gccgccgcgc
    50641 gcgccagttg gttagattta ccttcaacga agttatcaaa cgtgtgtttg acgtttacgt
    50701 tagaacgata ggtcggttct gccggagccg ggacgttatc ccaacccgag cgcgtagaag
    50761 gcgcagcacg ttgcggctgc gtttgcgcca cctgtgcagg ggccgcgacg ttgctcgtca
    50821 ctgccgcttg tggcgtttgc gtcaccggtt tggtgccgac ttcaaaacgc agctgtgggg
    50881 catccgctcc gcagaaactg gttagcagtc cattgatatt attaaggtac ttgtcccgta
    50941 cccaatcgag gacaaaacgg tttggcgcgt acagggccag cgtgttatcg ctcagttccg
    51001 cctgcaatgg gcgtatccac atactgaatt ctgtggctgg taactcatcc tgcaatcggg
    51061 caagacactg ctgccaaagc gaaagtgaca cggcggactc cactcgaaca aaagtcgata
    51121 atgactaagg ctgaaacatt catgattgtt gacgtacgtc gaaaagaccc tgcatgaggg
    51181 tgacgcacga accgctgtct gcggttatat gcccgatcaa gatcctgcaa aacgatcggg
    51241 accgcggatc atagcctaaa ctgcgcaaga gatcttctgt ttctcacaga ttttcccgat
    51301 ttatccacag gatgatccag aactcgctaa gtgtaaacga tcctgccgcg ggccgggcac
    51361 gatttacgcc gcatattgga aaatttaatg accatagaca aaaattggct taatcgatct
    51421 aataaagatc caggacgatc cttgcgcttt acccgtcagc ccgtataatc ctccacccgg
    51481 cgcgccatgc tggttttcac tggtgtgagg ccgtacgttt tccccgcgaa aaggtgcgga
    51541 aaagcgcggt aaataaggaa agagaattga ctccggagtg tacaattatt acaatccggc
    51601 ctctttaatc acccatggct tcggtgtcca tcgtttcatt tttcggcgga tatccaataa
    51661 agccattgaa tttattcaag tttaggtaga aatcgccatg aaacgcactt ttcaaccgtc
    51721 tgtactgaag cgcaaccgtt ctcacggctt ccgtgctcgt atggctacta aaaatggtcg
    51781 tcaggttctg gcacgtcgtc gtgctaaagg ccgcgctcgt ctgaccgttt ctaagtaata
    51841 aagctaaccc ctgagtggtt aagctcgcat ttcccaggga gttacgcttg ttaactccca
    51901 gtcaattcac attcgtcttc cagcagccac aacgggctgg cacgccgcaa attaccattc
    51961 tcggccgcct gaattcgctg gggcatcccc gtatcggtct tacagtcgcc aagaaaaacg
    52021 ttcgacgcgc ccatgaacgc aatcggatta aacgtctgac gcgtgaaagc ttccgtctgc
    52081 gccaacatga actcccggct atggatttcg tggtggtggc gaaaaaaggg gttgccgacc
    52141 tcgataaccg tgctctctcg gaagcgttgg aaaaattatg gcgccgccac tgtcgcctgg
    52201 ctcgcgggtc ctgatagccc tcattcgggt ctatcaacgc ctgattagtc cgctactcgg
    52261 gccgcattgt cgtttcactc caacctgttc aagctacgga attgaggcat tgcgcaggtt
    52321 tggagtgata aaaggcagtt ggttgacggt gaaacgcgta ttaaaatgcc accctttaca
    52381 ccctggtggt gacgatcccg tcccgcccgg accatttgat accagagaac actaacgatg
    52441 gattcgcaac gcaatctttt agtcatcgct ttgctgttcg tgtctttcat gatctggcaa
    52501 gcctgggagc aggataaaaa cccgcaacct caggcccaac agaccacgca gacaacgacc
    52561 accgcagcgg gtagcgccgc cgaccagggc gtaccggcca gtggccaggg gaaactgatc
    52621 tcggttaaga ccgacgtgct tgatctgacc atcaacaccc gtggtggtga tgttgagcaa
    52681 gccctgctgc ctgcttaccc gaaagagctg aactctaccc agccgttcca gctgctggaa
    52741 acttcaccgc agtttattta tcaggcacag agcggtctga ccggtcgtga tggcccggat
    52801 aacccggcta acggcccgcg tccgctgtat aacgttgaaa aagacgctta tgtgctggct
    52861 gaaggtcaaa acgaactgca ggtgccgatg acgtataccg acgcggcagg caacacgttt
    52921 accaaaacgt ttgtcctgaa acgtggtgat tacgctgtca acgtcaacta caacgtgcag
    52981 aacgctggcg agaaaccgct ggaaatctcc acctttggtc agttgaagca atccatcact
    53041 ctgccaccgc atctcgatac cggaagcagc aacttcgcac tgcacacctt ccgtggtgcg
    53101 gcgtactcca cgcctgacga gaagtacgag aaatacaagt tcgataccat tgccgataac
    53161 gaaaacctga acatctcttc gaaaggtggt tgggtggcaa tgctgcaaca gtatttcgcg
    53221 acggcgtgga ttccgcataa cgacggtacc aacaacttct ataccgctaa tctgggtaac
    53281 ggcatcgccg ctatcggcta taaatctcag ccggtactgg ttcagcctgg tcagactggc
    53341 gcgatgaaca gcaccctgtg ggttggcccg gaaattcagg acaaaatggc agctgttgct
    53401 ccgcacctgg atctgaccgt tgattacggt tggttgtggt tcatctctca gccactgttc
    53461 aaactgctga aatggatcca tagctttgtg ggtaactggg gcttctccat tatcatcatc
    53521 acctttatcg ttcgtggcat catgtacccg ctgaccaaag cgcagtacac ctccatggcg
    53581 aagatgcgta tgctgcagcc gaagattcag gcaatgcgtg agcgtctggg cgatgacaaa
    53641 cagcgcatca gccaggaaat gatggcgctg tacaaagctg agaaggttaa cccgctgggc
    53701 ggctgcttcc cgctgctgat ccagatgcca atcttcctgg cgttgtacta catgctgatg
    53761 ggttccgttg aactacgtca ggcaccgttt gcactgtgga tccacgacct gtctgcacag
    53821 gacccgtact acatcctgcc gatcctgatg ggcgtaacga tgttcttcat tcagaagatg
    53881 tcgccgacca ctgtgaccga cccgatgcag cagaagatca tgacctttat gccggtcatc
    53941 ttcactgtgt tcttcctgtg gttcccgtca ggtctggtgc tgtactatat cgtcagcaac
    54001 ctggtaacca ttattcagca gcagctgatt taccgtggtc tggaaaaacg tggcctgcat
    54061 agccgcgaga agaaaaaatc ctgattcggt gagttttcgc taaaataagg gcggtcagtt
    54121 gaccgccttt tttcttttcg tagggcggat aagcaccgca catccgccac acaaagcaac
    54181 aggaacatca tgagcgataa tgacactatc gtagcccagg ccacgcctcc gggacgtggc
    54241 ggcgttggca tcctgcgcat ctccggcctc aaagcccgtg aagttgccga aaccgtgctg
    54301 ggtaaactgc ctaagccacg ctacgccgat tatcttccgt ttaaagacgc cgatggcagc
    54361 gtgctcgatc aggggattgc gctatggttc cctggcccga actcgttcac cggcgaagat
    54421 gtgctggaac tgcaaggtca tggcggtccg gtgatcctcg acctgctgtt aaaacgcatt
    54481 ctgaccattc ccggcctgcg gattgcccgc cctggtgagt tttccgaacg cgcatttctg
    54541 aacgataaac tcgacttagc ccaggccgag gcgattgcgg atcttatcga cgccagctcg
    54601 gaacaggcgg cccgttcggc gctaaactcg ctgcaaggcg cattctccgc acgggtcaac
    54661 catctggtgg aagccctcac ccacttgcgc atttacgtcg aagcggcaat tgatttcccg
    54721 gatgaagaga tcgatttcct ctccgacggc aaaattgaag cccagctcaa tgacgtaatt
    54781 gccgatctcg acgcggtacg cgccgaagct cgtcagggta gtttgttgcg cgaagggatg
    54841 aaagtggtga ttgccggacg tcctaacgcc ggtaaatcga gcctgctgaa cgcgctggcg
    54901 ggccgtgaag cagcaatcgt aaccgatatc gccggaacca cgcgtgacgt gctgcgtgag
    54961 catatccaca ttgacggaat gccgttgcat atcatcgata ccgccgggct tcgtgaagcc
    55021 agtgacgaag ttgaacgtat tggtatcgag cgcgcatggc aggaaattga acaggcagac
    55081 cgcgtgctgt ttatggtcga tggcaccacg acagacgccg ttgatccggc agagatctgg
    55141 ccggaattta tcgcccgtct gccagcgaaa ctgccgatca ccgtggtgcg caataaagcc
    55201 gatatcaccg gcgaaacgtt ggggatgagc gaagtgaacg gtcatgcgtt aattcgtctt
    55261 tcggcgcgga ctggcgaagg cgtggatgta ctgcgtaacc atctcaaaca gagcatgggc
    55321 tttgacacca atatggaagg cggcttcctg gcgcgtcgtc gtcacctaca ggcgctggaa
    55381 caggcggcag agcatttgca gcaaggtaaa gcgcaactgt taggtgcctg ggcgggtgaa
    55441 ctgctggcgg aagagttgcg cctggcacag cagaacttaa gcgagatcac cggggaattt
    55501 acttcagacg acctactggg gcggattttc tccagtttct gtatcggtaa gtaaccgcgc
    55561 ttgcgaagcc gcattctgac tgtcagatgc ggcttcgctt cattgttagc actcctgtta
    55621 ttcctcaacc cttttttaaa cattaaaatt cttacgtaat ttataatctt taaaaaagat
    55681 atttaatatt gcgtcccgaa cgattgtgat tcgattcaca tttaaacaat ttcagaatag
    55741 acaaattcct tgagtattat actgtagcct cgtgtctttc gaggataagt gcattatgaa
    55801 tatcttacat atatgtgtga cctcaaaatg gtttaatatt gacaacaaga ttgtcgatca
    55861 ccgcccttga tttgcccttc tgtagccatc accagagcca aaccgattag attcaatgtg
    55921 atctatttgt ttgctatatc ttaattttgc cttttacaaa ggccatctct cgtttattta
    55981 cttgttttag taaatgatgg tgcttgcata tatatctggc gaattaatag atattgcaaa
    56041 tgtaatattc acagggatca ctgtaattaa aataaatgaa ggattatgta atggaaaact
    56101 ttaaacatct ccctgaaccg ttccgcattc gtgttattga gccagtaaaa cgtaccactc
    56161 gcgcttatcg tgaagaggca attattaaat ccggcatgaa cccgttcctg ctggatagcg
    56221 aagatgtgtt tatcgattta ctgaccgaca gcggcaccgg ggcggtgacg cagagtatgc
    56281 aggccgcgat gatgcgcggc gacgaagctt acagcggcag ccgcagctac tatgcgttag
    56341 ccgagtcagt gaaaaatatc tttggttatc aatacactat tccgactcac cagggccgtg
    56401 gtgcagaaca aatctatatt ccggtactga ttaaaaaacg cgagcaggaa aaaggcctgg
    56461 atcgcagcaa aatggtggcg ttctctaact atttctttga taccacgcag ggccatagcc
    56521 agattaacgg ctgtaccgtg cgtaacgtct atatcaaaga agccttcgat actggcgtgc
    56581 gttacgactt taaaggcaac tttgaccttg agggattaga acgcggtatt gaagaagttg
    56641 gcccgaataa cgtgccgtat attgttgcaa ccatcaccag taactccgca ggtggtcagc
    56701 cggtttcact ggcaaactta aaagcgatgt acagcatcgc gaagaaatac gatattccgg
    56761 tggtcatgga ctccgcacgc tttgccgaaa acgcctattt catcaagcag cgtgaagcag
    56821 aatacaaaga ctggaccatc gagcagatca cccgcgaaac ctacaaatat gccgatatgc
    56881 tggcgatgtc cgccaagaaa gatgcgatgg tgccgatggg cggcctgctg tgcatgaaag
    56941 acgacagctt ctttgatgtg tacaccgaat gcagaaccct ttgcgtggtg caggaaggct
    57001 tcccgacata tggcggcctg gaaggcggcg ctatggagcg tctggcggta ggtctgtatg
    57061 acggcatgaa tctggactgg ctggcttatc gtatcgcgca ggtgcagtat ctggtcgatg
    57121 gtctggaaga gattggcgtt gtctgtcagc aggcgggcgg tcacgcggca ttcgttgatg
    57181 caggtaaact gttgccgcat atcccggcag atcagttccc ggcacaggcg ctggcctgcg
    57241 agctgtataa agtcgccggt atccgtgcgg tagaaattgg ctctttcctg ttaggccgcg
    57301 atccgaaaac cggtaaacaa ctgccatgcc cggctgaact gctgcgttta accattccgc
    57361 gcgcaacata tactcaaaca catatggact tcattattga agcctttaaa catgtgaaag
    57421 agaacgcggc gaatattaaa gggttaacct ttacctacga accgaaagta ttgcgtcact
    57481 tcaccgcaaa actgaaagaa gtttaattaa aacactcaga gtggctataa ggatgttagc
    57541 cactctatta ccctacatcc tcaataacaa aaatagcctt cctctaaagg tggcatcatg
    57601 actgatcaat cagaaaaaaa acactctgca ttttggggtg ttatggttat agccggtaca
    57661 gtaattggcg gtgggatgtt tgctttacct gttgaccttg ccggagcctg gtttttttgg
    57721 ggcgctttta ttcttattat cgcctagttt tcaatgttac attcagggct attattatta
    57781 gaagcaaact taaattatcc cgtcggatcc agttttaaca ccatcaccaa agatttaatc
    57841 ggtaacacct ggaacattat cagcggtatt accgttgcct tcgttctcta tatcctcact
    57901 tatgcctata tctctgctaa tggtgcgatt attagtgaaa cgatatcaat gaatttgggc
    57961 tatcacgcta atccacgtat tgtcgggatc tgcactgcca ttttcgttgc cagcgtattg
    58021 tggataagtt cgttagccgc cagtcgtatt acctcattgt tccttgggct gaagattatc
    58081 tcctttgtaa ttgtttttgg ttctttcttc ttccaggtcg attactccat tctgcgcgat
    58141 tccaccagca ccactgcggg gacgtcttac ttcccgtata tctttatggc tttgccggtg
    58201 tgtctggcgt catttggttt ccacggcaat attcccagcc tgattatttg ctatggcaaa
    58261 aagaaagaca aactaatcaa aagcgtggtt ttcggttcgc tgctggcgct ggtgatttat
    58321 ctcttctggc tctattgcac gatggggaat atcccgcgag aaagctttaa ggcgataatc
    58381 tcctcaggcg gcaacgttga ttcgctggtg aaatcgttcc tcggcaccaa acagcacggc
    58441 attatcgagt tttgcctgct ggtgttctcc aacttagctg ttgccagctc gttttttggt
    58501 gtcacgctgg gattgttcga ttatctggcg gatctgttta agattgataa ctcccacgcc
    58561 gggcgtttca aaaccgtgct gttaaccttc ctgccacccg cgttgttgta tctgatcttc
    58621 ccgaacggct ttatttacgg gatcggcggt gccggactgt gcgccaccat ctgggcggta
    58681 ataattcctg ctgtactggc aatcaaagcc cgcaagaagt ttcccaatca gatgttcacg
    58741 gtctggggcg gcaatcttat tccggcgatt gtcattctct ttggcataac cgtaatttta
    58801 tgctggttcg gtaacgtctt taacgtgtta cctaaatttg gctaaatcct tcaggaagcc
    58861 agccattcgc tggcttcttg cctgtcagga aatcacttat gtccaaatgg caactcgcct
    58921 gattctcctt caccacgtat gctttgcgtc accttactat caggacgctt tagcccatgt
    58981 cccgcttttt gatttgtagt tttgccctgg ttttacttta tcccgcaggg attgatatgt
    59041 acctcgttgg tttaccgcgc atcgccgccg atctcaatgc cagcgaagcg cagttgcata
    59101 ttgcgttctc cgtttatctg gcggggatgg cggctgcgat gttatttgcc ggtaaagtag
    59161 ccgatcgttc agggagaaag cctgtcgcca tacccggcgc ggcgctattt attatcgcct
    59221 cggtgttctg ttcactggct gaaaccagcg cgttatttct tgcagggcga tttctacagg
    59281 gactgggcgc gggctgttgt tacgtagtgg cgttcgccat tttgcgcgac acgctggatg
    59341 atcgacgtcg ggctaaagtg ctgtcgttac tcaacggcat tacctgcatc attccggtgt
    59401 tagcgccggt gctcggacac ctgattatgc ttaaattccc ttggcagagt ctgttctgga
    59461 cgatggcaac gatgggcatc gcggtactga tgttgtcttt gtttatttta aaagaaacgc
    59521 gccctgcggc ccccacaact tcggacaaac cacgagaaaa tagcgagtca ctgcttaatc
    59581 ggtttttcct cagccgtgtt gttatcacca ccctcagcgt ttcggtgatc ctcaccttcg
    59641 tcaacacgtc gccggtattg ctgatggaaa tcatgggttt tgagcgcgga gaatacgcca
    59701 ccattatggc gttgaccgct ggcgtcagca tgaccgtttc attctccacg ccgtttgcgc
    59761 tgggaatttt taagccacgt acgttgatga tcacctcaca ggtgttattc ctggcggcgg
    59821 ggatcaccct tgctgtttca ccttcccatg cggtttcgct gtttggtatc acgctgattt
    59881 gcgccggttt ctcggtaggt tttggcgtag cgatgagtca ggcgttaggg ccgttttcat
    59941 tacgcgcggg cgtagccagc tcgaccttag gtattgcgca agtttgcggt tcgtcactgt
    60001 ggatttggct ggcggcggtg gttggtatcg gcgcatggaa tatgctgatc gggattctga
    60061 ttgcctgtag catagtgagc ctgttgctga ttatgttcgt cgcgcctgga cgcccggttg
    60121 ccgctcatga agaaatccat caccacgctt gatctcaatt tgctgctctg tctgcaactg
    60181 ctgatgcagg agcgcagcgt gaccaaagcg gcgaagcgga tgagcgtgac gccttcggcg
    60241 gtgagtaagt cgctggcaaa acttagaatg tggtttgatg acccgctctt tgtgaactca
    60301 ccgctgggcc tgtctcccac accgctaatg gtcagcatgg agcaaaatct ggcggaatgg
    60361 atgcaaatga gcaatcagtt gctggacaaa ccactccacg aaacgccgcg cggcctgaag
    60421 tttgaactgg cagcagaatc accgctaatg atgatcatgc ttaatgcgct gtcgaaacgg
    60481 atctaccaac gttacccgca ggcgaccatc aaattacgta actgggatta cgattcctta
    60541 gatgccatta ctcgtggtga agtggatatt ggtttttccg gtcgcgaaag tcatccccgt
    60601 tcgcgggagc tgttaagctc gctaccgtta gccattgatt atgaagtgct gtttagtgat
    60661 gtgccctgcg tctggttacg caaagatcat ccggcactgc atgaagcatg gaatctggac
    60721 acctttttac gttatccgca tatcagcatt tgctgggaac agagcgatac ctgggcgctg
    60781 gacaatgtgt tacaggagct ggggcgcgaa cgcacgattg ctatgagcct gccggaattc
    60841 gagcagtcac tgtttatggc agcacagccc gacaatctgc tactggcgac cgcgccgcgc
    60901 tactgccagt actacaatca actccaccaa ctgccgttgg ttgctcttcc tctcccgttt
    60961 gatgaaagcc agcaaaaaaa gctggaagtc ccttttaccc ttctgtggca taaacggaac
    61021 agccgtaatc cgaagatcgt ctggttacgg gaaaccatta aaaatcttta cgcgtcgatg
    61081 gcataaccga atcgtatgaa acgcgaccca atttcacaat gaaatgtaaa aaagttgtaa
    61141 taaacttatc tgaatcgaac ttttagccgc tttagtctgt ccatcattcc agtaaatgat
    61201 tactcttgta ttcataatgg accattaagc atggagcgaa aaatggcgac tcactttgcc
    61261 cgagggattt taacggaagg acatctgatt tctgttcgtc tcccctccct gtgtcatcaa
    61321 gaagcccgaa acattccccc tcatcgtcaa agccgttttc tggcgtccag aggtttactc
    61381 gcagaactga tgttcatgct gtatggcatt ggcgaattgc cggaaatcgt caccctgccg
    61441 aaaggaaaac cggttttcag cgataaaaat ttgccttcgt tttctatttc ctatgccggg
    61501 aatatggtcg gcgtggcgtt aacaaccgaa ggtgaatgtg gcctcgatat ggaactacag
    61561 cgtgcgacac gcgggtttca tagcccacac gcgcccgata accacacctt ttccagcaat
    61621 gaatcgctat ggatcagtaa acaaaacgat cctaacgaag cgcgggcgca gctcatcacg
    61681 ctgcgccgaa gcgtgctaaa actaaccggt gatgttttga atgacgatcc gcgcgatctg
    61741 caactactgc ccattgccgg acgcctgaaa tgtgctcatg taaatcatgt agaagcgtta
    61801 tgcgacgcgg aggacgtgct ggtgtggtcc gtggcggtca cgcccacgat tgaaaagctc
    61861 agtgtctggg agttagatgg caaacacggc tggaaaagcc tgcccgatat tcacagccgc
    61921 gccaacaacc cgaccagccg gatgatgcgt tttgcccagc tctctaccgt gaaggctttt
    61981 tcgccaaatt gatagacaac cacaggagtc atcatgtctg aaaaattgca ggtggttacg
    62041 ttactgggga gcctgcgcaa aggctcattt aatggcatgg ttgcacgtac cctgccgaaa
    62101 attgctccgg cgagcatgga aatcaatgcg ttaccttcca ttgccgacat tcccctgtat
    62161 gacgctgacg tacagcagga agacggtttt ccagcaacag ttgaagctct ggcggaacag
    62221 atccgtcagg ctgacggtgt ggtaatcgtc acgccggaat ataactactc ggtaccgggt
    62281 gggctgaaaa atgccatcga ctggctttcc cgcctgccgg atcaaccgct ggcaggtaaa
    62341 ccggtattga ttcagaccag ctcaatgggc gtgattggcg gcgcgcgctg tcagtatcac
    62401 ctgcgccaga ttctggtttt cctcgatgca atggtgatga acaagccaga atttatgggc
    62461 ggcgtgattc agaacaaagt tgatccgcaa accggggaag tgattgatca gagtacgctg
    62521 gaccacctga ccgggcaatt gaccgcattt ggtgagttta ttcagcgagt taagatctaa
    62581 ataaaaaacc cgccagcaat catgcatggc gggtttttaa cgcgctatcg attttagtga
    62641 gcgtcgataa agacaatctt caggataaac agcagcgcaa cgatgattac gcacgggcta
    62701 aggtcacgca gacgtccggt accaatcttc atcacgcagt aggagataaa gcccagcgcg
    62761 ataccttcag taatcgagaa gctgaacggc atcatcacag cggtaataaa cgccggaaca
    62821 gattcagtaa gatcctgcca gttcacgcga gcaagacttg aggtcatcaa cacgccaacg
    62881 taaatcagcg caccagctgc agcgtagcct ggcaccatcc ccgccaacgg cgacaggaag
    62941 ataaccagca ggaacagcaa accaacaacc actgccgtca gaccggtacg accgccaacg
    63001 gaaacgccgg aagaagattc aatatacgcc gtaacagaag aagtaccgat aaacgaaccg
    63061 gtcaccgaag agatactgtc gacatacagc gcctgcttca tgcgcgggaa tttacctttc
    63121 tcatcagcca gacctgcttt atcggtcacg ccaatcagcg taccggagga gtcaaacaag
    63181 ttgaccagca tgaaagagaa aatcacccct gccagcccaa ggttaaacga cccggctaaa
    63241 tctacatgac ccacgacact cattacactc ggcggcgcag aaacgatgcc attatagtgg
    63301 acatcaccca gcatccagcc cagcagcgtt gtcaccacga tggaaaccag caccgccgcg
    63361 tgaatattgc gcgaggccag aatggcgatg atgaagaagc cgaggatccc cagcagcacg
    63421 ctgtgagaag tcagattacc gatgctcacc agcgtttccg ggttagcgac aatcacacct
    63481 gcgtttttca gccccatcat gccaataaac agaccgatac cactggtgat gccaacacgt
    63541 agacttaccg ggatgttggc tatcatccag tagcgaacgc ggaaaatcgt cagtaacagc
    63601 agaccaatcg caccccagaa gattgcgccc atcccgactt gccacggcaa gcccatcgcc
    63661 tgtacaacga caaaagcgaa gaacgcattc aggcccattg ccggtgccag tgcaactggc
    63721 aggttagcaa acagccccat cataatactg ccgaatgcag caatcagaca ggtcgtgacg
    63781 aagacggcgc tggtatccat gccagcaacg ccaagaattt gcgggttaac aaaaacgatg
    63841 taaaccatcg tcaggaaggt ggtaaaaccg gcgatcactt cggtccgtgc cgtcgtgcca
    63901 tgttcgcgca gtttaaacac gcgttccagc atcccctggc cagaagcctg ggttgtgtgt
    63961 tgttgactca ttatctattt ccgaacaagg agggaaaatc cgtcgctatc gtataccaaa
    64021 atgagacaat atggacggct gaagataact ttttttcatt gtatttgctt atacggcaac
    64081 gattgcgtca tgcgaattgg catgacgaaa acgttaaact aacgataaag gaaagacatg
    64141 tcccagatag aagcggtatt tttcgactgc gacggtacgc tggtcgatag cgaggtcatt
    64201 tgctctcgcg cctatgtcac gatgttcagg gaatttggca ttcacgttga cctggaagag
    64261 atttttaccc gttttaaagg cgtaaaactc tacgaaatca tcgacatcat cagtaaagaa
    64321 cagggcgtga cgatggtcca ggccgatgcc gagcacgttt accgcgcaga agtagcacgt
    64381 ctgtttgata ccgaacttga ggacatcgca ggagcgaacg aattgctggc aagcattaac
    64441 gtgccgatgt gcgtcgtctc taacggtccg gtcagcaaaa tgcagcattc gcttggtaag
    64501 ctcaacatgc tgcactactt cccggaaaaa ctgttcagcg gctatgatat ccagcgttgg
    64561 aagcccgatc cggcattaat gttccatgcg gcaaaagcga tgaatgtgaa cgttgaaaac
    64621 tgcattctgg tcgatgactc cagcgccggt gcgcaatcag ggatagatgc cggaatggaa
    64681 gtgttttact tctgcgctga cccgcacaat aaaccgatcg ttcacccgaa agtcaccacc
    64741 tttacccatc tttcgcaatt accggaactg tggaaggcgc gtggttggga tattacggca
    64801 tagttattca cactcccttc acttactcca cttaaattgg cacgcaaagg taagtaaagg
    64861 ga
//
LOCUS       DAZFFE010000013        62492 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFFE010000013 DAZFFE010000000
VERSION     DAZFFE010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.013"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(184..2055)
                     /gene="ppiD"
                     /locus_tag="ACHJ69_001019"
     CDS             complement(184..2055)
                     /gene="ppiD"
                     /locus_tag="ACHJ69_001019"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO5014062.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2247..2537)
                     /gene="hupB"
                     /locus_tag="ACHJ69_001020"
     CDS             complement(2247..2537)
                     /gene="hupB"
                     /locus_tag="ACHJ69_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO5014063.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2728..5082)
                     /gene="lon"
                     /locus_tag="ACHJ69_001021"
     CDS             complement(2728..5082)
                     /gene="lon"
                     /locus_tag="ACHJ69_001021"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO5014064.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5270..6544)
                     /gene="clpX"
                     /locus_tag="ACHJ69_001022"
     CDS             complement(5270..6544)
                     /gene="clpX"
                     /locus_tag="ACHJ69_001022"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO5014065.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6670..7293)
                     /gene="clpP"
                     /locus_tag="ACHJ69_001023"
     CDS             complement(6670..7293)
                     /gene="clpP"
                     /locus_tag="ACHJ69_001023"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO5014066.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     ERMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7539..8837)
                     /gene="tig"
                     /locus_tag="ACHJ69_001024"
     CDS             complement(7539..8837)
                     /gene="tig"
                     /locus_tag="ACHJ69_001024"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO5014067.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9181..9498)
                     /gene="bolA"
                     /locus_tag="ACHJ69_001025"
     CDS             complement(9181..9498)
                     /gene="bolA"
                     /locus_tag="ACHJ69_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO5014068.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9803..10381
                     /gene="yajG"
                     /locus_tag="ACHJ69_001026"
     CDS             9803..10381
                     /gene="yajG"
                     /locus_tag="ACHJ69_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5014069.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10425..11900
                     /gene="ampG"
                     /locus_tag="ACHJ69_001027"
     CDS             10425..11900
                     /gene="ampG"
                     /locus_tag="ACHJ69_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO5014070.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFFWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNGNFISRTEYPAGYAFAMWTLAAGISLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12360..13307
                     /gene="cyoA"
                     /locus_tag="ACHJ69_001028"
     CDS             12360..13307
                     /gene="cyoA"
                     /locus_tag="ACHJ69_001028"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO5014071.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13329..15320
                     /gene="cyoB"
                     /locus_tag="ACHJ69_001029"
     CDS             13329..15320
                     /gene="cyoB"
                     /locus_tag="ACHJ69_001029"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO5014072.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15310..15924
                     /gene="cyoC"
                     /locus_tag="ACHJ69_001030"
     CDS             15310..15924
                     /gene="cyoC"
                     /locus_tag="ACHJ69_001030"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO5014073.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15924..16253
                     /gene="cyoD"
                     /locus_tag="ACHJ69_001031"
     CDS             15924..16253
                     /gene="cyoD"
                     /locus_tag="ACHJ69_001031"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO5014074.1"
                     /translation="MSHSNESSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQILVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16265..17155
                     /gene="cyoE"
                     /locus_tag="ACHJ69_001032"
     CDS             16265..17155
                     /gene="cyoE"
                     /locus_tag="ACHJ69_001032"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO5014075.1"
                     /translation="MIFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17304..18668
                     /gene="yajR"
                     /locus_tag="ACHJ69_001033"
     CDS             17304..18668
                     /gene="yajR"
                     /locus_tag="ACHJ69_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5014076.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQMADAGLPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(18796..19287)
                     /gene="yajQ"
                     /locus_tag="ACHJ69_001034"
     CDS             complement(18796..19287)
                     /gene="yajQ"
                     /locus_tag="ACHJ69_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO5014077.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIAHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            19455..20366
                     /gene="panE"
                     /locus_tag="ACHJ69_001035"
     CDS             19455..20366
                     /gene="panE"
                     /locus_tag="ACHJ69_001035"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO5014078.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHDVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAILNCPNGELRHHQQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            20329..20919
                     /gene="yajL"
                     /locus_tag="ACHJ69_001036"
     CDS             20329..20919
                     /gene="yajL"
                     /locus_tag="ACHJ69_001036"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO5014079.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(20973..22421)
                     /gene="thiI"
                     /locus_tag="ACHJ69_001037"
     CDS             complement(20973..22421)
                     /gene="thiI"
                     /locus_tag="ACHJ69_001037"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO5014080.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQSK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            22627..22869
                     /gene="xseB"
                     /locus_tag="ACHJ69_001038"
     CDS             22627..22869
                     /gene="xseB"
                     /locus_tag="ACHJ69_001038"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO5014081.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            22869..23768
                     /gene="ispA"
                     /locus_tag="ACHJ69_001039"
     CDS             22869..23768
                     /gene="ispA"
                     /locus_tag="ACHJ69_001039"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO5014082.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHIPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LTEQSLDTSALEALADYIIQRNK"
     gene            23793..25655
                     /gene="dxs"
                     /locus_tag="ACHJ69_001040"
     CDS             23793..25655
                     /gene="dxs"
                     /locus_tag="ACHJ69_001040"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO5014083.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELSLDATGMEAKIKAWLA"
     gene            25710..26684
                     /gene="yajO"
                     /locus_tag="ACHJ69_001041"
     CDS             25710..26684
                     /gene="yajO"
                     /locus_tag="ACHJ69_001041"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO5014084.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYQESNENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVTGFK"
     gene            complement(26738..27256)
                     /gene="pgpA"
                     /locus_tag="ACHJ69_001042"
     CDS             complement(26738..27256)
                     /gene="pgpA"
                     /locus_tag="ACHJ69_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO5014085.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVTAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(27234..28211)
                     /gene="thiL"
                     /locus_tag="ACHJ69_001043"
     CDS             complement(27234..28211)
                     /gene="thiL"
                     /locus_tag="ACHJ69_001043"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO5014086.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(28289..28708)
                     /gene="nusB"
                     /locus_tag="ACHJ69_001044"
     CDS             complement(28289..28708)
                     /gene="nusB"
                     /locus_tag="ACHJ69_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO5014087.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(28728..29198)
                     /gene="ribE"
                     /locus_tag="ACHJ69_001045"
     CDS             complement(28728..29198)
                     /gene="ribE"
                     /locus_tag="ACHJ69_001045"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO5014088.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(29287..30390)
                     /gene="ribD"
                     /locus_tag="ACHJ69_001046"
     CDS             complement(29287..30390)
                     /gene="ribD"
                     /locus_tag="ACHJ69_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO5014089.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQGL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGNDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(30394..30843)
                     /gene="nrdR"
                     /locus_tag="ACHJ69_001047"
     CDS             complement(30394..30843)
                     /gene="nrdR"
                     /locus_tag="ACHJ69_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO5014090.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            30994..31533
                     /gene="yajI"
                     /locus_tag="ACHJ69_001048"
     CDS             30994..31533
                     /gene="yajI"
                     /locus_tag="ACHJ69_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO5014091.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNKLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     MSVDQLGFVRIHDIQPVMQ"
     gene            31832..32716
                     /gene="tsx"
                     /locus_tag="ACHJ69_001049"
     CDS             31832..32716
                     /gene="tsx"
                     /locus_tag="ACHJ69_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO5014092.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQLRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKVKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGYANNGIKTRTNNSIASSHILALNYDHWHYSVVARYWHNGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(32754..33101)
                     /gene="yajD"
                     /locus_tag="ACHJ69_001050"
     CDS             complement(32754..33101)
                     /gene="yajD"
                     /locus_tag="ACHJ69_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO5014093.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(33231..34202)
                     /gene="secF"
                     /locus_tag="ACHJ69_001051"
     CDS             complement(33231..34202)
                     /gene="secF"
                     /locus_tag="ACHJ69_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO5014094.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(34213..36060)
                     /gene="secD"
                     /locus_tag="ACHJ69_001052"
     CDS             complement(34213..36060)
                     /gene="secD"
                     /locus_tag="ACHJ69_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO5014095.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPSEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDKGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(36088..36420)
                     /gene="yajC"
                     /locus_tag="ACHJ69_001053"
     CDS             complement(36088..36420)
                     /gene="yajC"
                     /locus_tag="ACHJ69_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO5014096.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(36443..37570)
                     /gene="tgt"
                     /locus_tag="ACHJ69_001054"
     CDS             complement(36443..37570)
                     /gene="tgt"
                     /locus_tag="ACHJ69_001054"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO5014097.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(37625..38695)
                     /gene="queA"
                     /locus_tag="ACHJ69_001055"
     CDS             complement(37625..38695)
                     /gene="queA"
                     /locus_tag="ACHJ69_001055"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO5014098.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDQRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            38788..39369
                     /gene="acpH"
                     /locus_tag="ACHJ69_001056"
     CDS             38788..39369
                     /gene="acpH"
                     /locus_tag="ACHJ69_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO5014099.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRNETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFT
                     CYAREQVMTILPDSPPRFIHLNNYLWSEQWLVRYRDMDFIQSVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMEQASRKAL"
     gene            complement(39374..41191)
                     /gene="malZ"
                     /locus_tag="ACHJ69_001057"
     CDS             complement(39374..41191)
                     /gene="malZ"
                     /locus_tag="ACHJ69_001057"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO5014100.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTSEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQLGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAIDVPDIGPQWAADQIFYQIFPDRFARNLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGIRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVTGITEAAKETQPDAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     SALFALYQRMIALRKKGQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(41347..42720)
                     /gene="proY"
                     /locus_tag="ACHJ69_001058"
     CDS             complement(41347..42720)
                     /gene="proY"
                     /locus_tag="ACHJ69_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO5014101.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(42796..44115)
                     /gene="brnQ"
                     /locus_tag="ACHJ69_001059"
     CDS             complement(42796..44115)
                     /gene="brnQ"
                     /locus_tag="ACHJ69_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO5014102.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIIWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(44522..45817)
                     /gene="phoR"
                     /locus_tag="ACHJ69_001060"
     CDS             complement(44522..45817)
                     /gene="phoR"
                     /locus_tag="ACHJ69_001060"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO5014103.1"
                     /translation="MLERLSWKRLVLELLLCCLLAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(45875..46564)
                     /gene="phoB"
                     /locus_tag="ACHJ69_001061"
     CDS             complement(45875..46564)
                     /gene="phoB"
                     /locus_tag="ACHJ69_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO5014104.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            46754..47956
                     /gene="sbcD"
                     /locus_tag="ACHJ69_001062"
     CDS             46754..47956
                     /gene="sbcD"
                     /locus_tag="ACHJ69_001062"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO5014105.1"
                     /translation="MRILHTSDWHLGQNFYSKNREAEHQAFLDWLLETAQAHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASVGHTPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDISQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            47953..51096
                     /gene="sbcC"
                     /locus_tag="ACHJ69_001063"
     CDS             47953..51096
                     /gene="sbcC"
                     /locus_tag="ACHJ69_001063"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO5014106.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRLDELQQEGSRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEYSTALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTCDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTLEQTQRNAALNEMRHRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSSSHPAVEAYQALEPGVNQARLLTLEKEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLNQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRMQQLTPILETLPQSDELPHSEETVALENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFYTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLTQHQQHRPGGLSLTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAVK"
     gene            51222..52406
                     /gene="araJ"
                     /locus_tag="ACHJ69_001064"
     CDS             51222..52406
                     /gene="araJ"
                     /locus_tag="ACHJ69_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO5014107.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSSVLAKP
                     LG"
     gene            complement(52675..53583)
                     /gene="mak"
                     /locus_tag="ACHJ69_001065"
     CDS             complement(52675..53583)
                     /gene="mak"
                     /locus_tag="ACHJ69_001065"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO5014108.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            53708..54619
                     /gene="rdgC"
                     /locus_tag="ACHJ69_001066"
     CDS             53708..54619
                     /gene="rdgC"
                     /locus_tag="ACHJ69_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO5014109.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSTAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(54700..54780)
                     /locus_tag="ACHJ69_001067"
     CDS             complement(54700..54780)
                     /locus_tag="ACHJ69_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014110.1"
                     /translation="MTQRPWSKLQHKTHNIAALRIIARCS"
     gene            complement(54777..55118)
                     /locus_tag="ACHJ69_001068"
                     /pseudo
     CDS             complement(54777..55118)
                     /locus_tag="ACHJ69_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(55266..55550)
                     /gene="ppnP"
                     /locus_tag="ACHJ69_001069"
     CDS             complement(55266..55550)
                     /gene="ppnP"
                     /locus_tag="ACHJ69_001069"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO5014111.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(55622..56299)
                     /gene="aroM"
                     /locus_tag="ACHJ69_001070"
     CDS             complement(55622..56299)
                     /gene="aroM"
                     /locus_tag="ACHJ69_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO5014112.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVFSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
     gene            complement(56557..56748)
                     /gene="yaiA"
                     /locus_tag="ACHJ69_001071"
     CDS             complement(56557..56748)
                     /gene="yaiA"
                     /locus_tag="ACHJ69_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO5014113.1"
                     /translation="MPTKPPYPREAYIVTIDKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(56798..57322)
                     /gene="aroL"
                     /locus_tag="ACHJ69_001072"
     CDS             complement(56798..57322)
                     /gene="aroL"
                     /locus_tag="ACHJ69_001072"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO5014114.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDLWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(57505..57963)
                     /gene="yaiI"
                     /locus_tag="ACHJ69_001073"
     CDS             complement(57505..57963)
                     /gene="yaiI"
                     /locus_tag="ACHJ69_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO5014115.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            58083..58892
                     /gene="proC"
                     /locus_tag="ACHJ69_001074"
     CDS             58083..58892
                     /gene="proC"
                     /locus_tag="ACHJ69_001074"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO5014116.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVTA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGTLKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(58909..60024)
                     /gene="adrA"
                     /locus_tag="ACHJ69_001075"
                     /gene_synonym="dgcC"
     CDS             complement(58909..60024)
                     /gene="adrA"
                     /locus_tag="ACHJ69_001075"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO5014117.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPSPGWWWLLLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(60126..60446)
                     /gene="psiF"
                     /locus_tag="ACHJ69_001076"
     CDS             complement(60126..60446)
                     /gene="psiF"
                     /locus_tag="ACHJ69_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO5014118.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(60565..61980)
                     /gene="phoA"
                     /locus_tag="ACHJ69_001077"
     CDS             complement(60565..61980)
                     /gene="phoA"
                     /locus_tag="ACHJ69_001077"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO5014119.1"
                     /translation="MKQSTIALALLLLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWQGPKAAYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(62081..62341)
                     /gene="iraP"
                     /locus_tag="ACHJ69_001078"
     CDS             complement(62081..62341)
                     /gene="iraP"
                     /locus_tag="ACHJ69_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO5014120.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLRHPRQ"
BASE COUNT        14972 a        16699 c        15555 g        15266 t
ORIGIN      
        1 cttattttat ttttttgtga tattaatcac atttaacatg gttgcttgtt tgttaacagt
       61 ggaatcacct taacggcagt gaaaacagta aacaaatggc aatgttcaga aatggaaaag
      121 gccgcgaaag cgaccttttc ttgttacagc tgcaaagcat ttttttgcga ggcggcttca
      181 ggattattgc tgttccagcg catcgccaat tttgattttc gcctctttac gcaggttact
      241 catcagagct tcaaagacga tttgtgcgtt gttctgggtg ataccctgca ccatcgcttt
      301 tttctgatct tccggcattg aaccttgttt cacttcatcc agcgccagca gaaccacatt
      361 accttgcata tcggtcgcca taccgtagct cggtttgtct ttcgctggca gtggcagtgc
      421 aaacgcagcc tggctaatcg ggtcacgacc ggaacggctt aaggttttcg gctcgccaaa
      481 tttcagaccg gcagcctgca tagcttccgc acctttgccg gctttcaaat caaccagcag
      541 tttctcagca tccactttcg cctgttgttc agctttgttg tgctgaacca gtgccttaac
      601 ttgttcctga acatctgcca acggtttcac cgcttccggt ttgtgctcgc tgatgcgcag
      661 cacgaatgcg cggtcgccgt ctacggtgat gatgtcagag ttgatgcccg gcgcgccgtt
      721 ttcacctacc agaccgccgt tgaagatagc gtcggcaact ggcttgaagt tcaactcttc
      781 cggcaggtta tctttgctga accaacccgt ctgagtggct ttaacgccgg cagcttgctc
      841 tgcaccggcc agagactcgg tgtcgttgct tgccgcatcg ctcactttct gctgcagcgc
      901 gtagtacgca tcgagggctt tttcgtgttt cactttcgcc gcaatgtcgt cacgtacttc
      961 gtctaacgat ttcactttcg ctggctgaat gtcgtccaga cgcacaatca ggaaaccgac
     1021 cgaagatttg ataacaccag acagttggcc tttttctttc agacccgcat ttttcagttc
     1081 gtccgggata gtggcatctt ctaaccaacc catatcaccg ccgttgcggg cagagataat
     1141 atcggcagat ttttctttgg ctaatgcagc aaaatcaccg cccttattca gctcatcaag
     1201 taccgctttc gcttcatctt cagttttggt ctggatgatg ctgtaacggg tacgctgcgg
     1261 ctgggtgaat tgatcctgat gctgatcgta gtagctctgg atatccgcat cgctaaccgg
     1321 ttgctgcatc gtcgcggcat ccagcttgat gtaactcacg cggaattgtt ccggcgtcat
     1381 gaaattgttt ttgttttgtt cgtagtagct ggcaatttcc tgttcggtca caggctgctt
     1441 cgccgccagc gcgttaacat cgatagtcgc ttcacgcacc acgcgttgtt gagctaccag
     1501 tgccgccagc tcgtcggttt cacctttcag cataaaatcg gtaccggcaa cgccgttaat
     1561 cagctgttgg gtagtgagct ggttacgcag cgcctgggcg tactgatcgg cggtcatccc
     1621 catctggttg aggataccgt tatagcggct gttatcaaat ttgccgtcaa cctggaaggc
     1681 tggggtcgcg aaaatcgcct gtttaacctg ctcatcgctg atacccagtt tcagctcacg
     1741 agcgtactga tccagaagcg cctcgtcgat cagacgattc agcacctgtt gacgcagggt
     1801 tttcatatag ccttcgttcg ctgccagctc ggagtattga tcgcccagct gttgctgcat
     1861 gcgattacgc tcgctgttga aggcgttctc gaattgccca cggctgattt cctggtcatt
     1921 cacttttgcg gcgtagttat tgcctccgcc aatcaggtaa ccactcacgc cggtcaatat
     1981 gaacgacacg ataatgatac cgaaaataat cttgagcacg agactgtttg cagccgtgcg
     2041 taagctgtcc atcatggtgt aacaacactc cgctgtagat gactgtgtac ctcacgcaac
     2101 gtatcgatgc gcgcaaaggc ctattgtgac aagaaacggg ggcaattgtc agcccacaag
     2161 acgcagaaag tcaccgaata caaataaaaa aggcacatca gtagatgcgc ccttgaactt
     2221 cgtcacatcc ccactgggga caacgcttag tttaccgcgt ctttcagtgc tttacctgca
     2281 cggaagctcg gtactttagc agcagcgatg gtgatctctt taccggtctg cgggttgcgg
     2341 ccagtacggg cagcacgctc tttaacggca aaagtaccaa aacctaccag tgctacatca
     2401 tccccttctt tcagagattc agttacggaa gcaataatag catctaacgc acggccagcc
     2461 gcagctttag agatatcagc ccctgctgca atcttgtcga tcaattgaga tttattcact
     2521 cttctcttcc tctttataat ttatatcgca cttgaatcct tcaaggtacg aacgcgcagc
     2581 agttatatca ggccagccat ccccttacaa caccccttaa taagggcaag cctgatccgc
     2641 catctaactt agcgagacaa aaaaaggctg gcaagcccga attagcctgc cagccctgtt
     2701 tttattagtg cattttgcgc gaggtcacta ttttgcagtc acaacctgca tgccagacgg
     2761 ttcattttgt agcgccagag tcagaacttc ctcaatgcgc ttcacaggat gaatgtccag
     2821 atcggcaatt acgttgtcag gaatctcttc cagatcgcgt ttattttcga acggaattaa
     2881 cactgtttta atcccgccgc gatgcgctgc cagtagtttt tctttcaaac caccgatcgg
     2941 cagtacctga ccacgcagag tgatctcacc ggtcattgcc acatcggcac gaaccgggtt
     3001 accggtcagg caagaaacca gcgcggtgca catggcaata ccggcactcg gaccatcttt
     3061 cggcgtcgca ccttccggta cgtggacgtg gatgtcacgt ttttcgtaaa aatcagggtt
     3121 gatccccagt ttttccgcac gcgcacgaac caccgttaac gccgcctgaa tagactcctg
     3181 catcacttcg ccgagcgaac cggtataggt cagtttacct ttccccggaa cgcacgcggt
     3241 ttcaatggtc agcaagtcac cgcccacttc cgtccacgcc agaccggtta cctggccgac
     3301 acggttttcg ttatccgcgc gcccatagtc gaaacgctga acaccgaggt agtcatgcag
     3361 gttatcgccg ttaatttcga tatgttttaa tgacttatcg agcagtaact gcttaaccgc
     3421 tttgcgacac agtttggaga tttcacgctc cagaccacgc acgcccgcct cacgggtgta
     3481 gtaacgaata atgccgataa tggcgctatc gtcgacggtc agctcacctt ttttcagtgc
     3541 attacgttca atctgcttcg gcagcaggtg acgtttggcg atgttcagtt tttcatcttc
     3601 ggtataaccg gagaggcgaa tcacttccat acgatccagc agcggtgccg gaatgttcat
     3661 ggagttcgac gtagcgacaa acattacgtc gctgagatcg taatccactt ccaggtagtg
     3721 gtcgctgaac gctacgttct gctctggatc cagcacttca agcagtgcgg aggccggatc
     3781 gccacgcatg tcagaagaca ttttgtcgat ctcatcgagc aggaacagcg ggtttttcac
     3841 gcccactttc gccattttct ggatcagttt acccggcata gaaccgatgt aagtacggcg
     3901 gtgaccacgg atttccgctt catcacgcac gccgcccagc gccatacgga catatttacg
     3961 cccggtggct ttggcgatgg actgacccag ggaggtttta cctacccccg gcggccctac
     4021 caggcagagg atcggtccct tgattttgtt gacacggctt tgaaccgcaa gatactcaag
     4081 gatacgatct ttcacgcgct ccagaccata atggtcggta tcaaggattt cctgcgcctg
     4141 acgcaggtct tttttgacct tgctacgcgc attccacggc acctgtacca tccagtcgat
     4201 atatccacgc actacggtcg cttctgccga catcggagac atcattttca gcttctgcag
     4261 ctctgcttcc gctttctctt ttgcctcttt cggcatcttc gccgcgtcga ttttgcgctt
     4321 cagggcttcg ttttcgtcag gcgcgtcgtc catttcaccg agttctttct gaatagcttt
     4381 catttgctcg ttcagatagt actcacgctg ggatttctcc atctgctttt taacgcggtt
     4441 gcgaatgcgt ttctcaacct gcagcagatc gatttccgat tccatcattg ccatcagata
     4501 ttccagacgt tcgttaacgt cggacatctc cagaacggac tgtttgtcag ccagtttcag
     4561 cggcatatgt gcagcaatgg tatccgccag acgcgccgga tcgtcgatgc tattcagcga
     4621 cgtcagcact tctggtggga tttttttgtt tagcttgatg tagccttcga actggctgat
     4681 tgcagtacgc accagcactt cctgttcccg ctcatcaatg gtcggcgact ccagatactc
     4741 cgccttcgca gaaaagtgtt cgccattgtc agagagcgca gaaatacgcg cgcgctgtaa
     4801 cccctcgacc agcactttga cggtgccgtc aggcagtttc agcatctgca atatagaggc
     4861 cacggtcccg acggtgaaaa gatcgtttac acccggctca tccgttgaag cttctttctg
     4921 cgcgaccagc ataatttttt tatcatggtc catcgccgct tccagacaac ggatggattt
     4981 ttcccgcccg acaaataagg ggatgaccat gtgcggataa accaccacat cgcgcagcgg
     5041 caatacgggg atttcaatgc gttcagaacg ctcaggattc atagagctct ctcttagttt
     5101 aatttccgcc aggtaatcag atgacacgac tgtgcttcac gccatctatt aacatgtacg
     5161 tcagtatatg gggatgtttc ccccacattc aacgccgaga atagaggaaa aattaaaggg
     5221 gagataaaat cccccctttt tggttaacta attgtatgga aatggttaat tattcaccag
     5281 atgcctgttg cgcttccggc ttaccataaa tcagcaacgg tttgctttga ccatcaatta
     5341 ccgactcgtc gataaccact ttttcgacat cttccatgga cggcagatcg tacatggtat
     5401 cgagcagtgc ggcttctacg atagaacgca gaccacgggc accggtttta cgcgccatcg
     5461 ctttcttagc gatagcatcc agcgcctcgt cacggaattc cagatccacg ccttccagat
     5521 taaacagcgc ctgatactgc ttggtcaggg cgtttttcgg ctctttgagg atctgaatca
     5581 gagcttcttc gctcagttca ttcaacgttg cgacaaccgg cagacgacca atgaactcag
     5641 ggatcagacc aaacttgatc agatcttccg gttcaacctg cgccagcagc tcgccttcgc
     5701 ttgctttgtc ggacttcgct tttaccgtcg cgccaaaacc aatgccggaa ccggtttcta
     5761 cacgatggga aatcacttta tccagaccgg caaacgcacc gccacagata aacaggatct
     5821 tagaggtatc aacctgcaag aattcctgtt gcggatgttt acgcccacct tgcggcggaa
     5881 cagcagctac cgtaccttcg atcaatttca acagtgcctg ctgtacgcct tcaccggaaa
     5941 cgtctcgggt aatggacggg ttgtctgact tacgagaaat cttgtcgatt tcatcgatgt
     6001 agacaatccc gcgctgcgct ttctgtacgt cgtaatcgca cttctgcaac agcttctgaa
     6061 tgatgttttc aacgtcttcg cccacataac cggcttcggt cagcgtggtt gcgtcagcca
     6121 tggtgaacgg aacgtccagc aggcgcgcca gcgtttcggc cagtagcgtt ttaccggaac
     6181 cggtcggacc gatcagcaga atgttacttt tgcccaactc gacgccattg ctggtatctc
     6241 cgttgcgcag acgtttgtag tggttgtata ccgcgaccgc cagcactttt ttcgcctgtt
     6301 cctgaccgat aacgtaatcg tccaggtggt tgcgaatttc atgcggcgtc ggtagcgcac
     6361 tgcgttcacg atgcggtgca acttctttaa tctcttcgcg aatgatgtcg ttacataaat
     6421 caacacattc gtcgcagata tacacggatg gaccggcaat cagcttgcgc acttcatgct
     6481 ggcttttgcc gcaaaaagag caatacagca atttgcctga gccatctttg cgtttatctg
     6541 tcatgggtca aaacctcttc tttgttcttt gtgccgcaca cgacgacgca aatgccattc
     6601 tcaggcgcaa gccgcttaca gcgttgtgcc gccctggata agtatagcgg cacagttgcg
     6661 cctctggcat caattacgat gggtcagaat cgaatcgacc agaccgtatt ccaccgcttc
     6721 aggggcagaa aggaagcgat cgcgctcggt atcacgttca atctgttcta atgattgacc
     6781 cgtatgaagc gccataagtt cattcatgcg ctctttaact ttcagaattt cacgggcatg
     6841 aatttcgata tcggtcgcct ggccctggta gccgcccaac ggctggtgaa tcatcacgcg
     6901 cgaattcggc aggcagaaac gtttaccttt tgcccctgca gtcagcaaga aagcacccat
     6961 cgaggccgcc tggcccatac agatggtgct gacatcaggc ttgataaact gcatggtgtc
     7021 atagatagac atcccggcag taatcacccc gcctggagag ttaatgtaca gatagatatc
     7081 tttttctggg ttttctgctt ccaggaacag catctgcgcc acaatcaggt tagccatgtg
     7141 atcttcaacc tggccagtca gaaaaatgac gcgttcctta agtagacgag aatagatatc
     7201 aaaagagcgc tcacctcgtg aggtctgttc aatgaccatc ggcaccagcg ccatatgggg
     7261 tgcaaagtta tctcgttcgc cgctgtatga catttccgtc tcctggataa aattgaaaaa
     7321 acctgctgta ccgattgtaa ccttatggac ggattatcag ctagtccctg tgttatgggt
     7381 acggcatcac catatttcaa gcataacaat cttttgttgt tacgctaaca ccgaaagagg
     7441 cttttcgcac ggttccatgc aaaattcatg acaaaaaaaa gcccaccacc tggcggtgac
     7501 gggcctttgt gcgaatttag agcgttatac tacgtaaatt acgcctgctg gttcatcagc
     7561 tcgttgaaag tggtttcttt ttcagtcact ttcgctttcg ccagtacagc ttcaacagcc
     7621 tgttcttcca gagcaacatt gcgcatgttg tccatcagtt ctttgttttt gctgtagaac
     7681 tcgataactt ctttcggatc ttcgtacgca gaagccatct cttcgatcag gcctttcacg
     7741 cgctcttcgt cagctttcag ctcgttggtg cggataactt cgcccagcag caggccaaca
     7801 actacgcggc gtttagcctg ttcttcgaac agttcgcgcg gcagttccag agcttgtttt
     7861 tcgttgccac cgaaacgctg tgcagcctgg cgacgcagaa cgtcgatttc gctgtcgatc
     7921 agcgcagccg gtacgtcgat gtcgttagct tttaccaaac cttcgatcgc ctgagactta
     7981 acgcggttac ggatggcgct cttcagctcg cgctccatgt ttttacgcac ttcagcgcgc
     8041 agaccttcta cggaaccatc ttcaacgccg aaacgtttga tgaattctgc agtcagttcc
     8101 ggcagttcac gctcttcaac tttcttcagg ttgatagcga atttcgctgc tttacctttc
     8161 aggttttctg cgtggtactc ttccgggaag gtcacgtcga tggtgaactc ttcgccagct
     8221 ttgtggcctt tgataccgtc ttcaaagccc gggatcatgc gaccctggcc catcgccagt
     8281 acgaaatcag acgctttacc gccttcgaac tcttcgccgt ctacagaacc ggtgaagtcg
     8341 atggtcacac ggtcttctgc ttcaacagcg ccgtcttttt ctttccaggt tgcctgctgt
     8401 ttacgcagag tatccagcat gccgtcaacg tcagcgtcgg tcacttcaac gatcggtttt
     8461 tcaacttcga tcgcttccag accctgcagt tcaacttccg gataaacttc aaactctaca
     8521 gagtaggtga agtcttcacc cagcttgtat tcgcccggaa cataggtcgg tgcgccagcc
     8581 ggattgattt tttctttaat gatggcgtca atgaagttac ggctcatcag gtcgcccagg
     8641 acgtcctggc gtacagacgc gccataacgc tgagcaacga tattcattgg cactttgcct
     8701 ttgcggaagc cgtcaatacg tacttttttc gcaacgttga ccagctcgct tttaacagcg
     8761 gtctcgatgc tgtcagcagc gatagtaatc gttacacggc ggccaaggcc ttgagtggtt
     8821 tcaactgaaa cttgcatctt gttacctcaa aaaatcacag tgctcggtca actcttactc
     8881 cgcaagcaca ggttgttggc ttcgcggaac cgggatgttc ttataatcaa tcacattccc
     8941 tgttaccaga atcatcccga aagcattcaa ataggacgca gcattatagc ggcatcactt
     9001 ttatgagtcg agaacggtta ttgcgcgctg tcgcgtattt tttcacaatt cccgggcgat
     9061 tttcgtctga atgctgggca aaaagcgcaa aatccagaca aaacaaaacg gcccgcaggc
     9121 cgtttttcat aaaatcttta gcaacatact ggaaaagcgc cgacagttgc aaatgcgttt
     9181 ttacgcgatg cttcctgctc cacgacaggg aggagaggca aagacggtgt cctgcaaccc
     9241 ttcccactcc ttaatagtgt aagtatgcag agccagcgca tgaacggtag tagagagttc
     9301 ctccgctaaa gtactgtaaa tcattcgatg acgattcaga aaacgttcac ccgtaaaacg
     9361 atcgctgacc agcacaactt taaaatggct ttcagagccg gctgggacat tgtgacgata
     9421 gctttcatcc actacttcga ggaatacggg ttggaacgcc gcccttaatt tttcttctat
     9481 ccgctcacgt atcatcatga aatatctcct ccgacaacgc tgagatgtca tccatccctt
     9541 taaatactag ccgcttttac cgtttccatt gcagcttaac aacaaatatt taggtttcgt
     9601 cctggttttt ccgtcaagcg tatgaagttt actgatttaa agattgaaac acaggcctcg
     9661 catcagcgtt tcttttacca ggggcgatta tttcgtcaac atgatgaatt tacatgcctt
     9721 acccacttcc cctcgccgtt ggcaatgtta tgatggcggg aatttttacc aaacacgctt
     9781 aagagtctct gagagcccga acatgttcaa aaaaatcctc ttcccgttag ttgctctgtt
     9841 tatgcttgca ggatgcgcaa aaccgccaac aactatcgaa gtttccccga cgattacact
     9901 gccacagcag gatccaagcc tgatgggcgt caccgtaagc attaatggtg ccgatcagcg
     9961 taccgatcag gcgctggcaa aagtcacccg cgataatcaa atcgttaccc tgaccgcctc
    10021 ccgcgatctg cgtttcctgc tgcaagaagt gctggaaaaa caaatgaccg cccgtggata
    10081 catggttggt ccgaatggcc cggttaatct gcaaatcatc gttagccaac tgtatgctga
    10141 cgtgtcccag ggcaacgtgc gctacaacat cgcgaccaaa gcggatatcg ccatcatcgc
    10201 taccgcgcag aatggcaaca aaatgaccaa aaactatcgt gccagctaca acgttgaagg
    10261 tgcgttccag gcctccaaca aaaatatcgc cgatgcggtt aacagtgtgc tgaccgatac
    10321 catcgctgat atgtctcagg acaccagcat ccacgaattc atcaagcaga acgcgcgtta
    10381 atttctgccc tctggcccgg tgcaagccgg gcctgtagac gcccatgtcc agtcaatatt
    10441 tacgtatttt tcaacagccg cgttcagcca tattgctgat cctgggtttt gcttccgggc
    10501 taccgctcgc cctgacgtcc ggcaccttac aggcctggat gacggtcgag aatatcgatc
    10561 tcaaaaccat tggtttcttc tctctggtag gccaggctta cgtttttaaa ttcttctggt
    10621 caccactgat ggaccgctac acgcccccat ttttcgggcg gcggcgcggt tggctgctcg
    10681 ccacgcaaat tctgttatta gtcgccattg cggcgatggg ttttctcgaa ccaggcaccc
    10741 agctccgctg gatggcggca ctggcggtgg tgatcgcttt ttgctctgcc tcccaggata
    10801 ttgtcttcga tgcgtggaaa accgatgtgc tcccggcaga agaacgtggt gcgggcgcgg
    10861 caatcagcgt gctgggttac cgtttaggga tgctggtttc cggcggctta gccctgtggc
    10921 tggcagataa atggctgggc tggcagggca tgtactggtt gatggcggcg ctgttgatcc
    10981 cctgtattat cgcgacgttg cttgcaccag aacccaccga caccattcct gtgcccaaaa
    11041 cgctggaaca agcggttgtt gcacctctgc gagatttctt tggtcgcaat aatgcctggc
    11101 ttattttgct tcttatcgtg ctgtataagc tgggcgacgc attcgctatg agcctgacaa
    11161 ccacgttttt gattcgcggc gtcgggtttg atgcgggtga agtaggcgtg gttaacaaaa
    11221 cgcttggctt actggcgacc attgttggcg cattgtacgg tgggattttg atgcagcgcc
    11281 tgtcgctgtt ccgtgcactg ctgattttcg gcattttaca aggtgcatct aacgccggtt
    11341 actggctgct gtcgattact gataagcatc tctacagcat gggtgcagcc gtctttttcg
    11401 aaaacctctg tggcgggatg ggtacatcag cctttgtcgc gctgttaatg acgctgtgta
    11461 ataagtcatt ttccgctact caatttgccc tgctctcagc gctttctgct gtagggcgag
    11521 tttatgtcgg ccccgtggcg ggttggtttg ttgaagcaca cggctggtcg acattctatc
    11581 tattctccgt cgccgctgcc gtaccagggc ttattttgct gctggtttgc cgccagacgc
    11641 ttgaatatac acgagtaaat ggcaacttta tctcccgtac cgaatatcct gcaggttatg
    11701 cctttgccat gtggacactg gcggcgggta tcagcctgtt ggccgtgtgg ttactgctgt
    11761 tgacaatgga cgcgctggat ttgacgcact tctctttcct gcctgcgctg ctggaagtcg
    11821 gggttttagt cgccctttct ggcgttgtgc ttggtggttt gctggattat ctggcgctac
    11881 gaaaaacaca tctgacgtaa tctgtaaata ttatttagct gtagtaatca ctcgccgtaa
    11941 ttattacggc taaataaaca tcagcatcgc ttattaatat ttgttctttt gtgcggctta
    12001 gcgtttggaa attattgacg caatttatga attctatttt tcttacacga ttcagctaat
    12061 gagtccttat tttctcacca cccagttgtc acctcaatga taattatttg ttaaataatt
    12121 gttttatttt atattgatta taccaattgc ccacccaaac tccttctgca ctcccctttc
    12181 gttataacgc ccttttgcaa cagcttctta aaatcaactt gatatgtttt gcaacatatg
    12241 tgacctgaca gccaaatcca agtaacagga atttaatcat gtttacagta atgtaacctt
    12301 cccgtaaaat gcccacacac tttaaacgcc accagatccc gtggaattga ggtcgttaaa
    12361 tgagactcag gaaatacaat aaaagtttgg gatggttgtc attatttgca ggcactgtat
    12421 tgctcagtgg ctgtaattct gcgctgttag atcccaaagg acagattggt ctggagcaac
    12481 gttcactgat actgacggca tttggcctga tgttgattgt cgttattccc gcaatcttga
    12541 tggctgttgg tttcgcctgg aagtaccgtg cgagcaataa agatgctaag tacagcccga
    12601 actggtcaca ctccaataaa gtggaagctg tggtctggac ggtacctatc ttaatcatca
    12661 tcttccttgc ggtactgacc tggaaaacca ctcacgctct tgagcctagc aagccgctgg
    12721 cacacgacga gaagcccatt accatcgaag tggtttccat ggactggaaa tggttcttca
    12781 tctacccgga acagggcatt gcaaccgtga atgaaatcgc tttcccggcg aacactccgg
    12841 tgtacttcaa agtgacctcc aactccgtga tgaactcctt cttcattccg cgtctgggta
    12901 gccagattta tgccatggcc ggtatgcaga ctcgcctgca tctgatcgcc aacgaacccg
    12961 gtacttatga cggtatctcc gccagctaca gcggcccggg cttctcaggc atgaagttca
    13021 aagctattgc aacaccggat cgcgccgcat tcgaccaatg ggtcgcaaaa gcgaaacagt
    13081 cgccgaacag catgtctgac atggcagcgt tcgaaaaact ggccgcgcct agcgaataca
    13141 accaggtgga atatttctcc aacgtgaaac cagacttgtt tgccgatgtg attaacaagt
    13201 ttatggctca cggtaagagc atggacatga cccagccaga aggtgagcac agcgcacatg
    13261 aaggtatgga aggcatggac atgagccacg cggaatccgc ccattaaagg ggttgaggaa
    13321 gaataaagat gttcggaaaa ttatcacttg atgcagtccc gttccatgaa cctatcgtca
    13381 tggttacgat cgctggcatt attttgggag gtctggcgct cgttggcctg atcacttact
    13441 tcggtaagtg gacctacctg tggaaagagt ggctgacctc cgttgaccat aaacgtctcg
    13501 gtatcatgta tatcatcgtg gcgattgtga tgttgctgcg tggttttgct gacgccatta
    13561 tgatgcgtag ccagcaggct cttgcctcgg cgggcgaagc gggcttcctg ccacctcacc
    13621 actacgatca gatcttcacc gcgcacggcg tgattatgat cttcttcgtg gcgatgcctt
    13681 tcgttatcgg tctgatgaac ctggtggttc cgctgcagat cggcgcgcgt gacgttgcgt
    13741 tcccgttcct caacaactta agcttctggt ttaccgttgt tggtgtgatt ctggttaacg
    13801 tttctctcgg cgtgggcgaa tttgcgcaga ccggctggct ggcctatcca ccgctatcgg
    13861 gaatagagta cagtccggga gtcggtgtcg attactggat atggagtctc cagctatccg
    13921 gtataggtac gacgcttacc ggtatcaact tcttcgttac cattctaaag atgcgcgcac
    13981 cgggcatgac catgttcaag atgccagtat ttacctgggc atcactgtgc gcaaacgtac
    14041 tgattattgc ttccttccca attctgacgg ttaccgtcgc gttgttgacc ctggatcgct
    14101 atctgggcac ccatttcttt accaacgata tgggtggcaa catgatgatg tatatcaacc
    14161 tgatttgggc ctggggccac ccggaagttt acatcttgat cctgcctgta ttcggtgtgt
    14221 tctccgaaat tgcggcaacc ttctcgcgta aacgtctgtt tggttatacc tcgctggtat
    14281 gggcaaccgt ctgtatcacc gtgctgtcgt tcatcgtctg gctgcaccac ttctttacga
    14341 tgggtgcggg cgcgaacgta aacgccttct ttggtatcac cacgatgatt atcgccatcc
    14401 cgaccggggt gaagatcttc aactggctgt tcaccatgta tcagggccgc atcgtgttcc
    14461 attctgcgat gctgtggacc atcggtttta tcgtcacctt ctcggtgggc gggatgactg
    14521 gcgtgctgct ggcagtaccg ggcgcggact tcgttctgca taacagcctg ttcctgattg
    14581 cgcacttcca taacgtgatc atcggcggcg tggtcttcgg ctgcttcgca gggatgacct
    14641 actggtggcc taaagcgttc ggtttcaaac tgaacgaaac ctggggtaaa cgcgcgttct
    14701 ggttctggat catcggcttc ttcgttgcct ttatgccact gtatgcgctg ggcttcatgg
    14761 gcatgacccg tcgtttgagc cagcagattg acccgcagtt ccacaccatg ctgatgattg
    14821 cagccagcgg tgcggtactg attgcgctgg gtattctctg cctcgttatt cagatgtacg
    14881 tttctattcg tgaccgcgac cagaaccgtg acctgactgg cgacccgtgg ggtggccgta
    14941 cgctggagtg ggcaacctct tccccgcctc cgttctataa ctttgccgtt gtgccgcacg
    15001 ttcacgaacg tgatgcattc tgggaaatga aagagaaagg cgaagcgtac aaaaagcctg
    15061 accactatga agaaattcat atgccgaaaa acagcggtgc cggtatcgtc attgcggctt
    15121 tctccaccat cttcggtttc gccatgatct ggcatatctg gtggctggcg attgttggct
    15181 tcgcaggcat gatcatcacc tggatcgtga aaagcttcga cgaggacgtg gattactacg
    15241 tgccggtggc agaaatcgaa aaactggaaa accagcattt cgatgagatt actaaggcag
    15301 ggctgaaaaa tggcaactga tactttgacg cacgcgactg cccacgcaca cgaacacggg
    15361 caccacgatg caggcggaac caaaatcttc ggattttgga tctacctgat gagcgactgc
    15421 attctgttct ctatcttgtt tgctacctat gccgttctgg tgaacggcac cgcaggtggg
    15481 ccgacaggta aggacatttt cgaactgccg ttcgttctgg ttgaaacttt cttgctgttg
    15541 ttcagctcca tcacctacgg catggcggct atcgccatgt acaaaaacaa caaaagccag
    15601 gtgatctcct ggctggcgtt gacatggttg tttggtgccg gatttatcgg gatggaaatc
    15661 tatgaattcc atcacctgat tgttaacggc atgggtccgg atcgcagcgg cttcctgtca
    15721 gcattctttg cgctggtcgg cacgcacggt ctgcacgtca cttccggtct tatctggatg
    15781 gcggtgctga tggtgcaaat cgcccgtcgc ggcctgacca gcactaaccg tacccgcatc
    15841 atgtgcctga gcctgttctg gcacttcctg gatgtggttt ggatctgtgt gttcactgtt
    15901 gtttatctga tgggggcgat gtaatgagtc attctaacga aagcagcggc gcatcccatg
    15961 gcagcgtaaa gacctacatg acaggcttta tcctgtcgat aatcctgacg gtaattccgt
    16021 tctggatggt gatgacaggg gctgcctctc cggccgtaat tctgggaaca atcctggcaa
    16081 tggcagtggt acagattctg gtgcatctgg tgtgcttcct gcacatgaat accaaatcag
    16141 atgaaggctg gaatatgacg gcatttgtct tcaccgtgct aatcatcgct atcctggttg
    16201 tgggctccat ttggattatg tggaacctca actacaacat gatgatgcac taagagcggc
    16261 ggttatgata tttaagcaat acctgcaagt aacgaaacca ggcatcatct ttggcaacct
    16321 gatctcggtg attgggggat tcctgttggc ctcaaagggc agcattgatt atcccctgtt
    16381 tatctacacg ctggttgggg tgtcactggt tgtggcgtcg ggttgtgtgt ttaacaacta
    16441 catcgacagg gatatcgaca gaaagatgga aaggacgaag aatcgggtgc tggttaaagg
    16501 cctgatctct cctgctgtct cgctggtgta cgccaccttg ctgggtattg ctggctttat
    16561 gctgctgtgg tttggcgcga atccgctggc ctgctggctg ggggtgatgg gctttgtggt
    16621 ttatgtcggc gtttatagcc tgtacatgaa acgtcattct gtctacggca cattgattgg
    16681 ttcgctctcc ggcgctgcgc cgccggtgat cggttactgt gcggtaaccg gcgagttcga
    16741 tagcggcgca gcgatcctgc tggctatctt cagcctgtgg cagatgcctc actcttatgc
    16801 catagccatt ttccgcttta aggattatca ggcggcaaac attccggtac tgccggtggt
    16861 gaaaggcatt tcggtggcga agaatcacat cacgctgtat atcatcgcct ttgccgttgc
    16921 cacgctgatg ctctctcttg gcggctacgc tgggtataaa tatctggtgg tcgccgctgc
    16981 ggtgagcgtc tggtggttag gcatggctct gcgcggctat aaagttgctg atgacagaat
    17041 ctgggcgcgc aagctgttcg gtttctctat catcgccatc actgccctct cggtgatgat
    17101 gtccgttgat tttatggtgc cggactcgca tacgctgctg gctgctgtgt ggtaacacaa
    17161 cctctcagtt aaaaaggtgc tacggcacct tttttcttag cattagaatc atatccctct
    17221 cgaaatattt actaaaaaat ccgcatgttt accccattcg tttgccgctt tacactagtc
    17281 gcgaatttaa aacagaggtg gtaatgaacg attataaaat gacgccaggt gagcggcgcg
    17341 cgacctgggg tttagggacc gtattttcat tacgcatgct gggcatgttc atggttctgc
    17401 cggttctgac cacgtacggc atggctctgc aaggtgccag cgaagcatta atcggtattg
    17461 ccattggtat ttatggtctg actcaggccg tttttcagat tccgtttggc ctgctttcag
    17521 accgcattgg tcgcaaacca ttaattgtcg gtgggctggc ggtgtttgcc gccggtagcg
    17581 ttatcgctgc gctctctgac tccatctggg gaattattct gggccgggcg ctacaaggct
    17641 ccggtgcgat tgccgctgcc gttatggcgc tgctttccga tctcacgcgc gaacaaaacc
    17701 gcaccaaagc gatggcgttt atcggcgtga gctttggcat tacctttgcc attgcgatgg
    17761 tgcttggccc gatcatcact cacaaacttg ggctgcacgc gctgttctgg atgatcgcta
    17821 ttctggcaac aaccggcatt gcgttgacca tttgggttgt gcccaacagt agcactcacg
    17881 tacttaatcg tgagtccgga atggtgaaag gcagtttcag taaagtgctg gcggaaccgc
    17941 ggctgctgaa actcaacttt ggcattatgt gtctgcatat tttgctgatg tcgacctttg
    18001 ttgccctgcc cggacagatg gctgatgcgg ggttaccggc ggctgaacac tggaaggtct
    18061 acctggcaac gatgttaatc gcctttggct cggtcgtgcc tttcattatc tacgctgaag
    18121 ttaagcgcaa aatgaagcaa gtctttgtct tctgcgtcgg gttgatcgtg gttgcggaaa
    18181 ttgtgttgtg gaacgcacaa acgcagttct ggcaactggt ggtcggcgtg cagcttttct
    18241 ttgtggcgtt taatttgatg gaagcccttc tgccttcact tatcagtaaa gaatcgccag
    18301 caggttacaa aggtacggcg atgggtgttt actccaccag ccagtttctt ggcgtggcga
    18361 ttggcggttc gctgggcggc tggattgacg gcatgtttga cggtcagggc gtatttctcg
    18421 ctggcgcaat gctggccgca gtgtggctgg cagtcgccag taccatgaaa gaaccgccgt
    18481 atgtcagcag cttgcgcatt gaaatcccgg cgaacattgc cgcaaacgag gcgttaaaag
    18541 tgcgtttgct ggaaactgaa ggcatcaaag aagtgttgat tgcagaagaa gaacattcag
    18601 cttatgtgaa aatcgacagc aaagtgacga atcgctttga ggttgaacag gcaattcgcc
    18661 aggcataaaa gcgaggcgaa attgcctgct gcgctacgct tatcaggcct accaagttcc
    18721 tgagttacaa acctttgtag gccgaatcag gcgttcatgc cgcatctgac atgaacaaag
    18781 cgcaggttgt cacgattaat cgcggaagtt tttgaactgg aacggctgac cgagatcgcc
    18841 accacgtacc atcgccatga cagcctgtaa atcatcacga gatttacccg ttacgcggat
    18901 ctcatcgccc tgaatttgcg cctgcacttt cagtttgctg tctttgatca ttttgacgat
    18961 tttcttctga gtcgcgctct caatgccctg tttcagtttc gcttccacaa accaggtttt
    19021 accgctatga gcgatatttt ccggtacatc cagtgaactg ccttcaatgc cgcgcttcag
    19081 caacttggca cgcagaatat ccagcaactg attgacctgg aagtcggact cgctcaacac
    19141 tttgatggtt ttgctggcgt cattcagctc aaatgaggct tcaacgttac ggaagtcaaa
    19201 acgggactcc acttcgcggc tcgcgttatc gaccgcgtta cgtgcttcct gaagatcaac
    19261 ttcagagaca atatcgaaag atggcatctt ttcctctccc ttcatttttg atgcgaagca
    19321 taatacctgc aaagttaaat aaccaacagt cctgcaccag gacgtatccc ttaattgtgc
    19381 atagtcgcaa cagcatgcac aattaaggga tagccttatt gaacacccgg agtggttgcg
    19441 ggtgaggagg aacaatgaaa attaccgtat tgggatgcgg tgccttaggg caattatggc
    19501 ttacagcact ttgtaaacag ggtcacgacg ttcagggctg gctgcgcgta ccgcaacctt
    19561 attgtagtgt gaatctggtt gagacagatg gttcgatatt taacgaatcg ctgaccgcca
    19621 acgatcccga ttttctcgcc accagcgatc tgctcctggt gacgctgaaa gcatggcagg
    19681 tttccgatgc cgtcaaaagc ctcgcgtcca cactgcctgt aaccacgcca atactgttaa
    19741 ttcacaacgg catgggcacc atcgaagagt tgcaaaacat tcagcagcca ttactgatgg
    19801 gcaccaccac ccatgcagct cgccgcgacg gaaatgtcat tattcatgtg gcaaacggta
    19861 tcacgcatat tggtccggca cggcaacagg acggcgatta cagttatctg gcggatattt
    19921 tgcaaaccgt gttacctgac gtcgcctggc ataacaatat tcgcgccgag ctgtggcgca
    19981 agctggcagt caactgtgtg attaatccac tgaccgccat cttgaattgc ccgaatggtg
    20041 aactacgcca tcatcaacaa gaaattatgc agatatgcga agaagtcgcg gcagtgatcg
    20101 aacgcgaagg gcatcatact tcagcagaag atttgcgtga ttacgtgatg caggtgattg
    20161 atgccacagc ggaaaatatc tcgtcgatgt tgcaggatat ccgcgcgctg cgccacaccg
    20221 aaatcgacta tatcaatggt tttctcttac gccgcgcccg cgcgcatggg attgccgtac
    20281 cggaaaacac ccgcctgttt gaaatggtaa aaagaaagga gagtgaatat gagcgcatcg
    20341 gcactggttt gcctcgcccc tggtagtgaa gagactgaag ccgtcaccac tatcgacctc
    20401 ttggttcgcg gcggtatcaa agtcaccact gccagcgtcg ccagcgatgg taacctggcg
    20461 attacctgct cgcgcggcgt gaagctgctg gcggatgcac cgctggttga agtggctgat
    20521 ggcgaatatg acgtgattgt gctgcctggt ggcatcaaag gcgcagagtg ttttcgcgat
    20581 agcaccctgc tggttgaaac cgttaaacag ttccaccgtt ccgggcgtat cgtcgcggct
    20641 atttgcgccg cgccagccac cgtgctggtc ccgcacgata tcttcccgat tggcaatatg
    20701 accggcttcc cgacgctgaa agacaaaata cccgccgaac aatggcagga caagcgcgtc
    20761 gtctgggatg cacgggtaaa attgctgacc agccaggggc caggtacagc tatcgacttt
    20821 ggtctgaaaa ttatcgacct gctggttggg cgcgagaaag cccatgaagt ggcatcacaa
    20881 ctggtgatgg cggcagggat ttataattat tacgagtagt gtcggatgcg gtaaacgtcg
    20941 catctgacca gatgcgacgt tacaaccaca tattacgggc gatacacctt cacattgtta
    21001 aaaccctgct cgcgcagata gagcgcctgc aggcggctca tcaccccgcg ctcacaccac
    21061 aacagccagg ttttgctctg gtcgagatcg ccaaatttgg tgctcagttt atagaacggc
    21121 agagaaacca catcaatccc ttcgactttc agtggcttat cttcctgttc atcgatagaa
    21181 cggatatcga ggatcacgtc gttcgggccg aagccattga cggtttccac ttccaccact
    21241 tcctgctcgg tctgctgggc gatttcgcgg atatcaacgt tattcgcttc ctccaccact
    21301 ttatcaagaa tgctgaagtc gaacttctct tcttccgctt caatcttcga tttaaccgct
    21361 ttcaccgtcg ggcttttgga aatcacgccg caatattccg gcatcgtgcg ggcaaagtct
    21421 tcggtgccaa tctggcgagc caggttgatg atgtgctctt tgtcgtaaga gatcagcgga
    21481 cgcaggatca gcgtatcgga gacgttatca atcaggcgca ggttggtcag cgtctggctg
    21541 gacacctggc cgagcgcttc accggtaacc agcgcctgta cgccgtaacg ttctgccact
    21601 ttggacgcgg cacgcaccat catacgtttg aggataacgc ccatctgacc gtcgtcgatt
    21661 ttctcgagaa tttcaccgac gaccggctca aagttaatgg cgacaaaacg cacgcggtgg
    21721 gagctaccaa aacggttcca cagataatgc gccacctgac gaacaccaat ttcatgcgcc
    21781 gcgccgccga gattaaagaa gcagtaatgc acgcggcagc cgcgacgcat caacatataa
    21841 ctggaaacac cggagtcgaa accaccggaa atgagcgaca acacatcttc ctgggtgccg
    21901 atcgggaaac cgccaatacc ttcgtagcgg cctttaatca gcagcagacg atcgtcttcc
    21961 acttccagat ggacagtcac ttccggattg gtcagcttca cgcgcgcgga ttcaatatgc
    22021 tgatttaaac cgccgccgac gtaacgttcc acatcaatcg agctaaaatc atgtttgccg
    22081 cggcgcttca cgcgtacgca gaaggttttg ccttccagct gatcgcgata ttgaaccaac
    22141 gctttctcga aaatatcgtg catgtctgta aacggcacgt cttcgacttc gagaatatgg
    22201 tggatacccg gaatacgggt cagggcgtcg cgaatagcca gacgctggtt ttcatctttt
    22261 gcgcgaactt cgatgttatc ccagtggcgg acgacagcga gcgtctcatc atagtgcttt
    22321 aaaacgttac gaatgttccc ggtaaggatt tttataaagc gcaagcgcac agattggctt
    22381 ttgatggtga tttccgggaa caatttaatg ataaacttca tggcggcaat ggttcgttgg
    22441 caagccttaa gcgacttgta tagggaaaaa tacagcagcc cacacctgcg gctgcatcca
    22501 ggcgcggaag tataccacta acatcgcttt gctgcgcaca tcaccttacc attgcgcgtt
    22561 atttgctatt tgccctgagt ccgttaccat gacggggcga aaaatattga gagtcagaca
    22621 ttcattatgc cgaagaaaaa tgaggctccc gccagctttg aaaaggcgct gagcgagctg
    22681 gaacagattg taacccgtct ggaaagtggc gacctgccgc tggaagaggc gctgaacgag
    22741 ttcgaacgcg gcgtgcagct ggcacgtcag gggcaggcca aattacaaca agccgaacag
    22801 cgcgtacaaa ttctgctgtc tgacaatgaa gacgcctctc taaccccttt tacaccggac
    22861 aatgagtaat ggactttccg cagcaactcg aagcctgcgt taagcaggcc aaccaggcgc
    22921 tgagccgttt tatcgcccca ctgccctttc agaacactcc cgtggtcgaa accatgcagt
    22981 atggcgcatt attaggtggt aagcgcctgc gacctttcct ggtttatgcc actggtcata
    23041 tgttcggcgt tagcacaaac acactggacg cacccgctgc cgccgtagag tgtatccacg
    23101 cttactcatt aattcatgat gatttaccgg cgatggatga tgacgatctg cgtcgcggtt
    23161 tgccgacctg ccatgtgaag tttggcgaag caaacgcgat tctcgctggt gacgctttac
    23221 aaacgctggc gttctcgatt ctaagcgatg ccgatatgcc ggaagtgtca gatcgcgaca
    23281 gaatttcgat gatttctgag ctggcgagcg ccagcggtat tgccggaatg tgcggtggtc
    23341 aggcattaga tttagacgct gaaggcaaac acatacctct ggacgcgctt gagcgtattc
    23401 atcgtcataa aaccggcgca ttgattcgcg ccgccgttcg ccttggtgca ttaagcgccg
    23461 gagataaagg acgtcgtgct ctgccagtac tcgacaagta cgcagagagc atcggccttg
    23521 ccttccaggt tcaggatgac atcctggatg tggtgggaga tactgcaacg ttgggaaaac
    23581 gccagggtgc cgaccagcaa cttggtaaaa gtacctaccc tgcacttctg ggtcttgagc
    23641 aagcccggaa gaaagcccgg gatctgatcg acgatgcccg tcagtcgctg aaacaactga
    23701 ctgaacagtc actcgatacc tcggcactgg aagcgctagc ggactacatc atccagcgta
    23761 ataaataaac aataagtatt aataggcccc tgatgagttt tgatattgcc aaatacccga
    23821 ccctggcact ggtcgactcc acccaggagt tacgactgtt gccgaaagag agtttaccga
    23881 aactctgcga cgaactgcgc cgctatttac tcgacagcgt gagccgttcc agcgggcact
    23941 tcgcctccgg gctgggcacg gtcgaactga ccgtggcgct gcactatgtc tacaataccc
    24001 cgtttgacca gttaatctgg gatgtggggc atcaggctta tccgcataaa attttgaccg
    24061 ggcgccgcga caaaatcggc accatccgtc agaaaggtgg cctgcacccg ttcccgtggc
    24121 gcggcgaaag cgaatatgac gtattaagcg tcgggcattc atcaacctcc atcagtgccg
    24181 gaattgggat cgcagttgct gccgagaaag aaggcaaaaa tcgccgcacc gtctgtgtca
    24241 ttggcgatgg cgcgattact gctggcatgg cgtttgaagc gatgaatcac gcgggcgata
    24301 tccgtcctga tatgctggtg gttctcaacg acaatgaaat gtcgatttcc gaaaatgttg
    24361 gcgcgctcaa caaccatctg gcgcagctgc tttccggtaa gctttactct tcactgcgcg
    24421 aaggcggaaa aaaagttttc tctggcgtgc cacccattaa agagctgctc aaacgtaccg
    24481 aagaacatat taaaggcatg gtagtgcctg gcacgttgtt tgaagagctg ggctttaact
    24541 acatcggccc ggtggacggt cacgatgtgc tggggcttat caccacgctt aagaacatgc
    24601 gcgacctgaa aggcccgcag ttcctgcata tcatgaccaa aaaaggtcgt ggttatgaac
    24661 cggcagaaaa agacccaatc accttccacg ccgtgcctaa atttgatccc tccagcggtt
    24721 gtctgccgaa aagtagcggc ggtttgccaa gctattcaaa aatctttggc gactggttgt
    24781 gcgaaaccgc agcgaaagat aacaagctga tggcgattac tccggcgatg cgtgaaggtt
    24841 ccggcatggt cgagttttca cgtaaattcc cggatcgcta cttcgacgtg gcaattgccg
    24901 agcaacacgc agtgaccttt gctgcgggtc tggcgattgg aggctacaaa cccattgttg
    24961 cgatctactc caccttcctg caacgcgcct atgatcaggt gctgcatgac gtggcgattc
    25021 aaaagcttcc ggtcctgttc gccatcgacc gtgcgggcat tgttggtgct gacggtcaaa
    25081 cccaccaggg cgcttttgat ctctcttacc tgcgctgcat accggaaatg gtcattatga
    25141 ccccgagcga tgaaaacgaa tgtcgccaga tgctctatac cggctatcac tataacgatg
    25201 gcccgtccgc ggtgcgctac ccgcgcggca acgcagttgg cgtggaactg acgccactgg
    25261 aaaaactgcc aattggcaaa ggcattgtga agcgtcgtgg tgagaaactg gcgatcctta
    25321 actttggtac gctgatgcca gaagcggcga aagtcgctga atcgctgaac gctacgctgg
    25381 tcgatatgcg tttcgtgaaa ccgcttgatg aagcgttaat tctggaaatg gccgccagcc
    25441 atgaagcgct ggttaccgta gaagaaaacg ccattatggg tggtgcaggc agcggcgtga
    25501 acgaagtgct gatggcccat cgtaaaccag tacccgtgct gaacattggt ctgccggact
    25561 tctttattcc acaaggaact caggaagaaa tgcgcgccga actcagcctc gatgccaccg
    25621 gtatggaagc caaaatcaaa gcctggctgg cataatccct accctactcc tgctatgctt
    25681 aagaaattat tcatggcagg agtggcagca tgcaatacaa ccccttagga aaaaccgacc
    25741 ttcgtgtttc ccgactttgc ctcggctgta tgacctttgg cgagccagat cgcggtaatc
    25801 acgcatggac actgccggaa gagagcagcc gtcccatcat taaacgtgcg ctggaaggcg
    25861 gcataaattt ctttgacacc gccaatagct attccgatgg cagcagcgaa gagatcgtcg
    25921 gtcgcgcact gcgggatttc gcccgtcgtg aagacgtggt cgttgcgacc aaagtgttcc
    25981 atcgcgttgg tgatttaccg gaaggattat cccgtgcaca aattttgcgc tctatcgacg
    26041 acagcctgcg ccgtctcggc atggattatg tcgatatcct gcaaattcat cgctgggatt
    26101 acaacacgcc gatcgaagag acgctggaag ccctgaacga cgtggtaaaa gccgggaaag
    26161 cgcgttatat cggcgcgtca tccatgcacg cttcgcagtt tgctcaggca ctggaactac
    26221 aaaaacagca cggctgggcg cagtttgtca gtatgcagga tcactacaat ctgatttatc
    26281 gtgaagaaga gcgcgagatg ctgccgctgt gttatcagga aggcgtggcg gtgattccat
    26341 ggagtccgct ggcgcggggc cgtctgacgc gtccttgggg agaaaccacc gcacggctgg
    26401 tgtcggatga agtggggaaa aatctctacc aagaaagcaa tgaaaatgac gcacaaattg
    26461 cagaacgctt aacgggcgtc agtgaagaac ttggtgcaac gcgagcacaa gttgcgctgg
    26521 cctggttgtt gagtaaaccg ggcattgccg caccgattat cggaacttct cgggaggaac
    26581 agcttgatga actgctaaac gcggtggata tcaccttgaa gccggaacag atcgccgagc
    26641 tggaaacgcc gtataaaccg catccggtca caggatttaa ataggtatgc aggcctgatg
    26701 agacgtgaca agcgtcacat caggcatcgg tgcacaacta cgacagaata cccagcggcc
    26761 agtgatgacc gataaaatac aggatgccag cggagatcac cccggcgaca atatcgtcaa
    26821 tcatgatccc catgccgcca tgtacattgc gatcaaacca gcggatcggc cacggcttcc
    26881 acatatccag aatacggaaa atcacaaatc cggcggtaac ccactgccag tcattggtcg
    26941 gcagcgccat gagcgtaatc cacataccaa taaattcgtc ccagacaata ctgccgtgat
    27001 cgtgcacgcc catgtctttc gccgtttgat gacaaagata gacgccgata cagatcccca
    27061 gcattaccac cagcgaatag agctgccagg gcaaaaaagt catcagatac cagaagggaa
    27121 tcgccgccag cgaccccatc gtgccaggaa cgatcgggct taatccactt ccgaatccga
    27181 cagcaagcag atgccacgga ttactcattt tcaggcgact tttcgcgaca tctttatggc
    27241 gtggcaaaat ggtcatatcc tttccagtct aacgtgacag gttcgccgtc acgaataaaa
    27301 caaagccctt cgatatcggc ggtcatttgc ccgatacagg taaacggtac gcccaggtgt
    27361 ccgagagcca catccagcgc gccacggttc agttccggca ccgtgaaaca caactcgtaa
    27421 tcttcaccgc cagagagcgc ccagcgcagc gcctgttccg gttcaacatg gcgagaaagc
    27481 gcatcagaaa acggcagcaa tgccaggtca atacgtgcgc cgcagtcgct ggctttcacg
    27541 atatgcccga gatcggaaat cagaccgtca gagagatcga tagctgaatt tgccagatcg
    27601 cgcagtgcct gcccctgtaa aatacgcggc gacggacgga gatgacgttt gatcaagtag
    27661 tccgcatctt tagcatcggc aacctgcaaa cggttttgca aaatcgccag cccggcagcg
    27721 ctatcgcccg gtgtaccggt cacatagatc cagtcacccg gtttcgcccc agagcgcgtt
    27781 aaggctcgtc ccatcggaac aaagccgtgg atacccaacg tcattgataa tggcccacgc
    27841 gtggtatcgc cgccaatgag ttgcatatcg taataattga gaagatcaaa caagctgtcg
    27901 ctgaaggact caagccacgc ttcgtctacg tccggtaagg ttaatgccag cgtcagccag
    27961 gccggatcgg cccccatcgc tgccagatcg cttaggttca ccgccagtgc tttataagcc
    28021 agatcagcag gatcgatatc agggaggaag tggttgcccg ccaccagcgt atcagtgctg
    28081 atcgccaggg tctgtttctc agggatattg agaagagcgc aatcgtcgcc gatgcccagt
    28141 tcgacatcaa gacgagaact tcttacacgg tcaaaataac gggcaatcag ggagaactcg
    28201 ccacatgcca tacgttatgc ctcagcaggt aaaaaagaaa aggccggcga tcatggaacg
    28261 gtcttccgtg aatctaccgg cctggatatt actttttgtt agggcgaatc acaggtgctg
    28321 ctttatcgag tacgccgttg acgaacttat ggctgtcttc tgcgccgaac gatttcgcca
    28381 gttcgatcgc ttcgttaatg gccactttgt atggcacatc gctacgttta gacagctcgt
    28441 acagcgcaat gcgcagtact gctttttcta cctggcccag ttcttccagc agacgggaca
    28501 ggtacggctt catcagtccg tcgaggtatg cggtgttagt cgccaccccg gccagcagct
    28561 cacggaagta caggacgtca acgtctttta catcctgttc agccaggaac tggtattcaa
    28621 catcagcgat gtcgttctgg gacaactgcc aagagtagag cgcctggacg gcacactcac
    28681 gagcgcggcg acgagcagca ggtttcacgg atttcccctt actaatttca ggccttgatg
    28741 gctttcaata cattaatcat ttcaagcgcg gtcagtgcag cttctgcacc tttgttgcca
    28801 gctttggtgc cagcacgttc gatcgcttgt tcaatgcttt cagtggtcag aacaccaaaa
    28861 gcaaccggaa tttcgctgtc ctgggcaaca tgcgccaggc cgttgcttgc accaccagcg
    28921 acatattcaa agtgggcagt gccaccacga ataaccgtac ccagcgcaat caccgcgtcg
    28981 tatttaccgg ttttagccag tgcacccgcc gccagcggca gctcataggc acctggcacc
    29041 caaacaacgg taatgttttc atcttttacc tgaccgatac gtttcagtgc gtcaattgca
    29101 ccttccagca ggctgtcatt gataaagttg ttgaaacgcg cgatggtgat ggcgacgcga
    29161 gcgtccgggg tagcaacgtt agcttcaata atgttcattt tcttccttcg ggttcgagta
    29221 tggccccgca ggggggcgga ttttagcata atatttcgtg cgctgcttcc ctttcgagcc
    29281 gggagatcat gcacccacta aatgcaggca aacatccggg cctacatgac gtatctcttt
    29341 gaatttaaat tggggagcgt cggctaattt ctcaagccct ggcagcgtgc ataatccacg
    29401 ggcgtcgttg cctaatagtt taggtgcgat atagacaatc agctcatcga ctaaacccgc
    29461 ctgtagcaat gcgccagcga gcgttggccc cgcttccacc cagatgctgt taatctgctg
    29521 tttacccagt tgcatcatta gtacaaccag atccaggtga cctttatgct ctggaatcag
    29581 caaggtacgc accgtttccg gccactcacg agaatcttcc tgcgtacgcg cgaaccaggt
    29641 ttcgccgggc tgctgcacaa tgcgatgttc cggcgtcacg cgattttggc tatcaatcac
    29701 aatacgtacc ggctggcgga gattttgttg cgggtagagc ccctgagttt gttcatccag
    29761 ttcagaccaa cgcactgtta aggctggatc atccgccagc accgtggcgc tgctggttaa
    29821 aatggcatga ctttgcgcgc gcagtcgttg tacgtcgcgt cgcgcctgag gcgaagtgat
    29881 ccactggctt tcgccactcg ccatcgccgt gcgaccatca agcgatgcgc caagttttaa
    29941 ctgaatataa ggaaagccgg tgcgcatccg cttgagaaag cctttattca attgctcagc
    30001 ttcactcatc atcagcccgt ggctgacgtc aatgccagcc tgttgcagac ggtaaagtcc
    30061 acgtccagcg acctgcggat tggggtcttg cattgcagca accacgcgcg ccacgcccgc
    30121 agcaattaat gcatcacaac acggtggcgt acgaccatga tggctacagg gttcgagtgt
    30181 gacatacgcc gttgcgcctt tggctttttc gccggccatg cgcaacgcgt gtacctcggc
    30241 atgaggttca cccgctcgtt gatggtaacc ttcaccgaca atttcgccat ctttgacaat
    30301 gacgcacccg acattcgggt tgggatgcgt ggtaaaacgt cctcgttgcg ccagctttag
    30361 cgcccgcgcc atgtaatact cgtcctgcac ggcttagtcc tccaggcgcg cgatctcttc
    30421 gccaaattct ttgatatctt cgaaactgcg gtagacagag gcaaaacgga tataggcgac
    30481 tttatcgagc tttttcaatt gctccatcac cagattgcca atcatcttgc tcggcacttc
    30541 gcgctcaccg gtggcgcgca gctgcgattt tatatgattg attgccattt cgacgtcatc
    30601 ggaactcacc ggacgttttt ccagcgcccg cagcattccg ctacgtaatt tctcttcatt
    30661 aaacggttca cgcacgtcgt tgctttttac aacacgcggc ataaccagct ccgccacttc
    30721 aaaggtggtg aaacgttcat tacacaccag acactgccga cggcggcgta cggatgaacc
    30781 ctcacccacg agacgagagt caattacctt agtgtccacg gcgaaacaga atgggcaatg
    30841 catacggcgt cctgaccagg tggttaacag aaatctattt taccctgaac tgacgataca
    30901 acaaaggcgc aacgcttttg agataaagga tcgatgcttt cactgctttt accctctacc
    30961 attaggctaa ttgcgacgag aaagggacga cctatgaata ctaacgtttt tcgactgctc
    31021 ctgctgggaa gcctgttcag ccttagcgcc tgtgtgcagc aaagtgaagt gcgacagatg
    31081 aaacacagcg tcagcacgct gaaccaggag atgacgcaac tcaataaaga aacggtcaaa
    31141 atcactcagc aaaacaagct gaatgcaaaa tccagcagtg gggtttacct tctgcctgga
    31201 gcaaaaacac cagcaagact ggaaagccag atcggtactt tacgtatgtc gctggtgaat
    31261 attacgcctg acacagatgg cactaccctg acgctacgta ttcagggcga atccaatgac
    31321 cctttacccg cattcagcgg aaccgttgaa tatgggcaaa ttcagggaac aatagacaac
    31381 tttcaggaaa tcaatgtgca gaatcaattg attaacgcac ctgccagcgt cctcgccccc
    31441 agcgatgttg atattccgtt acagttaaag ggtatgtctg tggatcagtt aggctttgta
    31501 cgtatccacg acattcaacc tgttatgcag taaacgtatt gcggggcgat attgtgcgtc
    31561 ccgcaacatc tttctccgtc attttgttac tccgcttaca tcacctggat tgatagtaaa
    31621 agtttgcaac aagggtgaaa gtcagtacaa tccccgcccg aatgtgtgta aacgtgaacg
    31681 caatcgatta cgtaaatgat agaactgtga aacgaaacat atttttgtga gcaatgattt
    31741 ttataatagg ctcctctgta tacgaaatat ttagaaacgc aatttgcgcc tttttcactc
    31801 ccgcaaggga ttttcaaaca gtggcataca tatgaaaaaa acattactgg cagccggtgc
    31861 ggtactggcg ctctcttcgt cttttactgt caacgcagct gaaaacgaca aaccgcagta
    31921 tctttccgac tggtggcacc agagcgttaa cgttgtcgga agctatcaca cccgtttcgg
    31981 accgcagctc cgcaacgata cctaccttga gtacgaagca ttcgctaaaa aagactggtt
    32041 cgacttctat ggttatgcgg atgcgccggt attcttcggc ggtaactccg atgcaaaagg
    32101 tatctggaac cacggttctc cgctgtttat ggaaatcgaa ccgcgtttct ccattgacaa
    32161 gctgaccaat actgacctta gcttcggtcc gttcaaagag tggtacttcg cgaacaacta
    32221 catttacgac atgggtcgta ataaagatgg ccgccagagc acctggtaca tgggtctggg
    32281 taccgacatc gatactggtc tgccgatgag cctgtccatg aacgtctatg cgaaatacca
    32341 gtggcagaac tacggcgcag cgaacgaaaa cgagtgggac ggttaccgtt tcaaagtgaa
    32401 gtactttgtg ccgattaccg atctgtgggg cggccagctg agctacatcg gcttcaccaa
    32461 cttcgactgg ggttccgacc tgggcgatga cagcggttat gcgaacaacg gtattaaaac
    32521 acgtaccaat aactctattg cgtccagcca tattctggcg ctgaattacg atcactggca
    32581 ctactctgtc gttgcccgtt actggcataa cggtggtcag tggaatgacg atgcagaact
    32641 gaacttcggc aacggcaact tcaacgttcg ctctaccggc tggggtggtt atctggtagt
    32701 aggttacaac ttctaattgt gagaatgccg gatttcatat ccggcatttt ttatcacttc
    32761 ttcttgttca tcatcgcttt caaatcagcg aatgggttgt acttcgcttc accgacatct
    32821 ttctgcgcat cttcccctgc gataacggtc gtaccatact gatccgcttc ggtgtatttc
    32881 gaatgctcat gatcgtggca atagagacaa agtaattccc agttactgcc atcttccggg
    32941 ttattagtat ggtcgtgatc aatgtggtga acggtaagtt cacgcaggtt ggaataaaca
    33001 aactcgcggg aacagcgacc gcagacccac ggatagattt ttaatgcttt ttcgcgatag
    33061 ccgctttcta accgcgcata gttttttggg atgatagcca tgaaaaagtt acctggatga
    33121 aatgtagggt tacgcgcagt ttcccaagat gcggggattt tcgcaatcac cttacatcaa
    33181 aaaaaatccc gatcttctga ccgggatttt caacatcagc tgaaacttga ttaaggcaga
    33241 attgacggct gatccgcccc ttctttttcc actttctgct gcaacatgtg ttcgcgcttc
    33301 atacccagtt tcagagccag cgcagatgcc acatagatgg aagatgcagt accgatggaa
    33361 acaccgataa gcatggtcaa tgagaaccct tccagcaccg ggccaccgaa gaggtacaac
    33421 atcaggataa ccatcaaggt agtaccagat gttatcaagg tacggtgcag cgtctgggtc
    33481 aaggacacgt taaagatttc gtaaggcgta ccgcgacgga tcttgcggaa gttctcacga
    33541 atacggtccg ataccacgat actgtcgtta agtgagtaac cgataaccga catcaacgat
    33601 gcaacgatgg tcaggtcaat ctcaatatgg aataacgaca aaatacccaa cgtaatgata
    33661 acgtcgtgcg ccagtgcaat aacgacccct gccgccagtc gccactcaaa gcggaaacca
    33721 acgtacacaa ggatagaaag cagcgccgcc atcaacgcca tcgcaccggt ttgcgcaagg
    33781 tctgccccca cgctcggacc gacgaactca atacgcttca ctgccgcatt ctgattggtg
    33841 gattcgttaa tcaccttcag aacctggctg cccagcacct gaccgccggt ttcgccttca
    33901 gcaggcggca tacggaccat gatgtcgtgg ctgctaccaa agttttgcag catcggctct
    33961 tcaaaaccgg ctttttgcaa tgcatcacgc attacgtcaa tttcagccgg tttttcgagc
    34021 gtaatttcaa taaccgtacc accggtaaaa tccagccccc agttaaagcc gcgcacgccc
    34081 ataataacga tagcggcgat taacagcaga ccagagatgc cgaaagccca gtagtcccag
    34141 cgcataaagt catagacttt acggccgtgg tttagttgtt caacagtata ttcctgtgcc
    34201 acatcgcact cctcagattg acagcttctt gacgcgcttg ccgccatata gcaggtttac
    34261 gatggcacgc gtaccgacaa tcgcggtaaa catcgacgtc gccacaccga taccggtagt
    34321 aatcgcgaac cctttaattg ccccggtacc cactgcatac aggatgatga ctttaatcag
    34381 cgtggtgatg ttcgcatcga agatggaact gaatgcgcca cgataacctt tatcaattgc
    34441 ctgttgaaca gtacgtccgt tgctcaactc ttctttaata cgttcgttga tcagtacgtt
    34501 cgcatcgacc gctaccgcaa gggttaagac gatacccgcg atacctggca tactcagcgt
    34561 tgcgcctggc aacagcgaca taatgccgac gatcaagatc aagttggcaa tcagagcact
    34621 ggtcgcaatc agaccaaact tcttatagaa gatgatcatg aacagaatag aaaccagcag
    34681 accggcaagg caagcctcca gcccctgctc aatgttctgc atacccaggg ttgggccaat
    34741 ggtacgttct tcaacaatct gaatcggcgc gatcaacgca ccggcacgca gcagcagtga
    34801 aagctgacgc gcttcgcttg ggttattgat gccagtaata cggaagctgt tacccagacg
    34861 agactggatg ttggcgatat taatcacctc ttcctgtttc accagaaccg cacgaccgtt
    34921 tgcatctttc ttaccgctgt ctttgtactc cacaaacaga gttgccatcg gtttgccgat
    34981 gttgtccttg gtgaagttag acatgatgtt accacccgcg ctatcgagcg agatgttaac
    35041 ctgcggctgg ttgtattcgt cctggctgga agtggagtcg gtgatatggt caccggtcag
    35101 aattacgcgt ttgtacagca caaccggctg accttcgcgg gtctgtttca cttcagagtc
    35161 gcccggtacg cggccagatg cagcagcggc ctgatcaacg ttggtgttta ccagacggaa
    35221 ttccagcgtt gccgtcgcac ccagaatctc tttcgcacgc gcagtgtcct gaatacctgg
    35281 cagttcaaca acgatacggt cagcaccctg acgctgaacc accggctccg ccacgccaag
    35341 ttggtttaca cggttacgca ggatattgat gttctgctgc accgcatatt cacgcgcttc
    35401 actcagacga gcatcgctca ttaccgcacg cagctggttg ctgccctggc tgctaatcac
    35461 caggtccgga tggcgcttgc tcagatacgc aatggcttca tcacgagctt tagcatcgcg
    35521 gaaagtgatg ctcaggccgt agttgttttc tttacgaacg gtggtatacg ggatgccctt
    35581 ttcacgcagg tcgctgcgca ggctatcgat attttgttcc tggagtttgc caagcgcggt
    35641 gtccatatcc acttccatca ggaagtgaac gccgccacgc aggtcaaggc cgagcttcat
    35701 cggctcagca tgaatagctg ccagccagcg cggcgttgcc ggggcaaggt taagcgccac
    35761 gacgtatttg tcacccataa cgcccattaa tgcttcacga gcgcgcagct gggtgtcagt
    35821 ggagtcgaag cgcgcaagaa tagcgccctc ttccagtgcc acagacttag cagttatttt
    35881 ttcttcttgt aacgttttct ggacctggat cagcgtttgc tcactggcgg cgactccgcg
    35941 cgcaccagtg atctgaacag ccggatcctc accaaacagg ttgggaagcg catacagcag
    36001 accgatgaca atcaccacga tcagcatgac gtacttccac aaaggataac ggtttaacac
    36061 ggcaattccc ttagggaaaa attttaatta cagcgccttc atggtgcctt tcggcagaac
    36121 ggcagctacg aagtcacgtt taataactac ttcagtggtg tcattcagtg cgatagcaat
    36181 gtagccgttt tccgctactt tggttacgcg accgaccagg ccaccgttcg tcagcacttc
    36241 atcaccttta gcgatggagt ccatcagctt tttgtgttct ttggtgcgct tctgctgtgg
    36301 acgcaggatc atgaaataga aaatcagacc gaataccacc agcatcaaaa tcaaagacat
    36361 cgggctacct tgcgccggtg cacccgttgc cgctaccgca tcagaaataa aaaagctcat
    36421 taaatttccc tcattattaa tattaatcaa cgttcaaagg tggtacttct cgcccctgac
    36481 gctggtaaaa atcagttacg aagctctcta atttaccctc ttcaatagcc ttgcgtaaac
    36541 ccgccatcaa acgctggtag taacgaaggt tatgaatggt gttgagtcgc gcgcctaata
    36601 tttcgttgca acggtcgaga tgatgcaagt aagcgcgtga ataattgcga caggtgtagc
    36661 aatcacactc aggatcgagt gggccagtat cgctcttata cttcgcattg cggattttca
    36721 ccacgccatc ggtcacgaac aaatgaccat ttcgggcgtt gcgggttggc attacgcagt
    36781 caaacatatc gataccgcga cgtacgccct caaccaggtc ttctggttta ccaacgccca
    36841 tcaggtaacg cggtttgtct gccggaattt gcgggcatac atgctccaga atgcggtgca
    36901 tatctgcttt cggctcaccc acagccagac cgccgacagc gtagccatca aaaccgatat
    36961 ctaccagacc tttaacagaa atatcacgta aatcttcgta aacactgccc tgaatgatac
    37021 caaacagcgc atttttgttt ccgagactgt caaaacgctc acggctacgc ttcgcccaac
    37081 gcagagacat ctccatggag cgttttgcgt aatcccagtc agcaggatac ggcgtacact
    37141 catcaaagat catgacgata tccgaaccaa gatcgtactg aatctccatc gacttttccg
    37201 ggtcgaggaa aatcgggtcg ccgttgatcg ggttgcggaa gtgaacgccc tgttcggtga
    37261 ttttacgaat atcaccaagg ctgaagacct ggaagccgcc ggagtcggta aggattggtc
    37321 ctttccactg cataaaatcg tgcagatcgc cgtgcagttt catgatttcc tggcccgggc
    37381 gcagccacag gtggaaggtg ttgccgagga taatttgcgc gccagtggct tcaacttctt
    37441 ccggcgtcat cccttttacg gtgccgtaag tgccaacagg cataaaacaa ggcgtttcca
    37501 ctacgccacg atcaaagacc aggcggccac ggcgtgcgcg accgtcggtg gtgtccagtt
    37561 caaatttcat tttttctcca ctacgtcaga aaaacagtcc aacgtttaaa ccagcgccgc
    37621 ggaattactc cccaacgcgc tcattaattg cctgcggatt gtacgtgata aacatcgcat
    37681 caccgtaact aaaaaagcga tatttctctt ctaccgccgc tttataggca ttcatggtgt
    37741 gttgataacc agcaaatgcc gaaaccagca taatcagcgt cgattccgga aggtggaagt
    37801 tcgtcaccag cgcatcgacc actttgtact ggaagcccgg atagataaag atttgggtat
    37861 cgtcaaagaa cggttcaatg agatcgtttt tcgctgcctg agccgcactt tccagcgaac
    37921 gtactgaggt ggtaccaacc gcaatcaccc ggttaccgcg cgctttcgcc gccagtaccg
    37981 cgtctaccac atcctgcggc acttcagcgt attccgagtg catgatgtga tcttcaatgg
    38041 tgtcgacgcg caccggctga aaggtgcccg caccaacgtg caacgtcaca aacgccatct
    38101 ccacgccttt ggcgcgtaat ttttccagca aaggttcgtc aaagtgcagt ccggcggtcg
    38161 gtgctgctac cgcgccgggt ttttcgctat aaaccgtttg ataaagttcg cggtctgcat
    38221 cttcgtccgg acggtcgatg tacggcggca gcggcatatg gccgatgctg ttgagaatat
    38281 ccagcaccga acgttgatca ttaaattcga cttcaaacag tgcgccgtgg cgcgcggtca
    38341 ttgttgcgtt aatactttcg tcatcgccca gcagcagttc tgcgccaggt tttggcgctt
    38401 tcgaggcgcg aatatgcgca agaatgcgtt tgtcgtcgag catccgctca accagcactt
    38461 caatcttgcc gccgctggct ttacgcccaa acagacgcgc cgggatcacg cgggtattat
    38521 taaaaaccag aagatcgccg gggttgagct tatcgagtaa atcggtgaaa gtaccgtgcg
    38581 tcagcgcgcc cgtcggcccg tccagcgaca gtaaacgaca gctactgcgt tcaggcatgg
    38641 gatagtgggc aatcagggat tcgggcaatt caaaagagaa atcggtaacg cgcatgacac
    38701 tgactcagac taaaataaga ggcgggtagt ctagtgccgg ggcgctctcc ctgcaacctt
    38761 tacccccctt tggatagaat ataatcgatg aattttttag ctcacctgca tttagcccat
    38821 ctcgcggaaa gctcgctttc cggcaattta ctggctgatt tcgtacgcgg aaatcctgaa
    38881 gaaagttttc cgcccgacgt cgtggctggt attcatatgc atcggcgaat cgacgtatta
    38941 accgacaatc tgccagaagt ccgcgaagcg cgggagtggt ttcgtaatga aacgcgccgt
    39001 gtcgcgccta ttacgctgga tgtgatgtgg gatcactttc tgtcccgcca ctggtcgcag
    39061 ctgtcgcccg attttccgct acaggaattt acctgttatg cccgggagca agtgatgact
    39121 attcttccag actcaccgcc acgctttatc catctgaaca actacttatg gtcagagcag
    39181 tggttggtgc gctatcgcga tatggatttc atccagagcg tgttaaacgg catggcaagc
    39241 cgccgcccac gtctggatgc cctgcgtgac tcctggtacg atttagacgc ccattacgat
    39301 gctctcgaaa cccgcttctg gcagttttat ccgcggatga tggagcaggc gtcacgcaag
    39361 gcgttataag acgttaattc atccacaccg tagccgaaat ggcaggcaaa gccagaatcc
    39421 cgtccgtcag ttgcccgtga ccttctttgc gttgccattg cgcgacatta agcaacggtg
    39481 acgcgggtag caccacttca caggcttcgc cacggttgat tgccaccagt acacgctgct
    39541 gattcagcac gcggacaaat accaccacgt tatcttccgc atacagcacc tgacagccgc
    39601 cacgacgcaa cgcctgacct ttcttacgca gcgcaatcat tcgctggtac agcgcgaata
    39661 acgccgaatc ctgcttttcc acctgccagg ggaacggctt acggcaaaac ggatcgtttt
    39721 tgccatccag tcccacttca tcaccgtaat aaatgcacgg tacaccaggc caggtgaaca
    39781 gccagaccac cgccaggggc aggcgcgcaa catcccgacc gagcagcgtt ttaaatcgcg
    39841 cagtatcgtg gctgtcgagc tgattaaaca tacgtaattg ttgttgatga gaaagccctg
    39901 cgcggtaatt atccatccag gccatacagg tttgggcgtc aatttgctgc ggatcgtaag
    39961 agatatcggt gttggcaaga aatccccaca acgggaatgt gaagccgcga tagttcatgg
    40021 cggcatcttc cacatcggcc tgtaaccatt gccttgcatc gccaaaatgt tcgccgacaa
    40081 tatacgcatc cggctgggtt tctttcgccg cttcggtgat cccggtaacg tgctgcaaat
    40141 tattgcgcgc cccacccgct tcccccagca tatgcaccac atccagccgc cagccatcca
    40201 tattccacgg cgctttcagc cagtggcgga caatactgtc ttccccgcga taaatttcat
    40261 tcaccaaact ttccgactga taatccagct tcggcaagct ggcatagccc agccagtcga
    40321 gcgccgtgcc atcatcacta aacgagtacc agtcacgcca gggtgattcg gggttgtgac
    40381 aagctccccc cgtgccacga ttgtgcctgt caaaccaggc atgggaatcg ccactgtggt
    40441 taaacacgcc gtccagcacc agtcgtattc ccagctgctg cgtattgtgt cgcaaacgca
    40501 gcaacgcccc atcaccgcca aactgcggat cgacatggcg ataatcctcg gtatcgtatt
    40561 tatgcacgct gggagcttta aacaccggat tgagatacag cgccgtcacg ccaagttttt
    40621 tcagatacgg cagtttttca cttatcccgt ccagatcgcc gccatagaac gttgatccgc
    40681 cagcctgcgc cgtgaccggt tcatcccagt cacgcaatat gatctcttgt ccggctgcgt
    40741 gatggtaata gacatgatcc tgttcagctt cacgaggaag attacgggcg aaacgatcag
    40801 ggaagatctg ataaaaaatc tgatccgcag cccattgtgg gccgatatcc ggtacatcaa
    40861 tggcaaactg ctccagtcgt gctggcggca ttcggctgaa gccttgcggt gtaaaccaac
    40921 gctggcgatc gtgccacagc aatttgaaac tataacgccg ccgaggttgt ccgctggaga
    40981 gatcgatcgc cgcccgccat gcggtgacgc caagctgcgg ctgactgcgc tgcttatgca
    41041 tcggtactga catttcttcg ttatcgtgtt ctgtacgcag cataatgcgc tgcggtgggt
    41101 cttcgctcgt cagccacagt gtaatgagca gttgatcttt gctttgttta acaaatgggg
    41161 gcaccggcag atgccatgca tttaacatca taattcccct gtgatgaaaa tctgatcacc
    41221 ttgccatagc ctaatgcagg atcactcatc atctgaagga tatttggggg aggaggacgg
    41281 aggcggataa ctttcggaat agccggatca ggatttcctc ctgaaccggc ttacagaacg
    41341 caaggattac tggttttcag ccagctgacg atcgtggcgg cgcttaaaca tccagccaat
    41401 caacagcaca acaatccacg cgaaaccgac atacagcgag atacgcgtat cagggtgata
    41461 accaatcaac ccgataataa agagcaggaa aatcaaaccg ccgatggtcg ttgctacccc
    41521 acccggcact ttaaatttca gcgccttaac ttcttctggc ggcaaacggc gacggaaggc
    41581 gatttgcgac agcaggatca taatccacac ccacaccgtg gcgaaggttg ccagcgaagc
    41641 gatcaccagg aagacgtttt ccggcatgat gtagttcaga tacaccgcaa acagcagcgc
    41701 ggtagtcatc accagcaccg taacccacgg aataccgcga cgcgaggttt tgctgaaaat
    41761 ttttggcgcg ctgccctgct ctgccatacc gtggagcata cggcctacac caaatacgtc
    41821 actgttaatt gccgacagcg aagcggtcag cacaacaaag ttaagaatgc tggcggcaaa
    41881 ggtaatgccc atatgctgga acgttagcac aaacgggcta ccggcagtgc caacctgatt
    41941 ccacgggtag atagacataa tgacgaacag cgtaccgacg tagaacacca gaatacgcat
    42001 cggcacggag ttaatcgcac gcggaatcga tttctcagga tctttcgctt caccggcggt
    42061 aatcccgata atttcgatcc caccgtaagc aaacatcacc atttgcaacg acatcaccat
    42121 accaagccag ccgttactga agaagccgcc gttgctccac aggttatgaa taccggtcgg
    42181 ttgcccgccg ttgccaatcc cccagatgat gatgccgaaa ccggcgacaa tcatgatgat
    42241 gatagtggcg actttaaaga acgagaacca gaactccagc tcaccgaata ccttcacgct
    42301 catcaggttt acggcgcaaa tgatcagcac cacactcagt acccaaatcc agtgcggcac
    42361 cgtcgggaac cagacaccca tatagatacc aaaagcggtc acatcggcga tggcgacaat
    42421 aaggatttca aagcagtagg tccagccggt aatgtaacct gccagcgggc cgaggttttc
    42481 ctgcgcataa cgcgagaaag agctggctgc cgggttatgt accgacattt cccccagcgc
    42541 acgcataatg atatacgccg cgataccacc gataatatag gccaacaaca cgcttggacc
    42601 ggccattttg atggcatctg ccgaaccgta aaacagcccg gtgccaattg ctgaacccag
    42661 tgccataaag cgtatgtgtc gggtacttag cccacgcttt agcttgttct tactttccat
    42721 caattcctaa cccatcaaca caataaaaaa ccacggggca tcgcagcccc gtggttaaaa
    42781 caaatgttca gtgatttagt gagcgctgga ggtcacctga cgacctgccg cgcgatccca
    42841 gataatggcc agaaccacca tcaccactgt tggcattaac cacgccagac cttgttcggc
    42901 cagcggtaaa cgctgcgccc aggacggtaa gatatcgctg aatgcagatg ctttgatccc
    42961 gtcgagaata ccaaaaagca ggctgataaa catcggcgga gcaatcacgc gggacgaatt
    43021 atgccaccat gagcgggtaa aacttaatac aaccagtgcg atacacggcg gataaatggc
    43081 tgtcagcacc ggtacagaga tctgaatcag ctggctcaag ccgaggttag acaccaccat
    43141 cgagaagccg ccgaggataa acaccagcgt acgataagag agcggtacgt actgggcgaa
    43201 gaattctgca caagcacagg tcaggccaac cgccgtgacc aggcaggcga tgaagattaa
    43261 cgccgccagc aggaagctac cgccgccgcc aaaggtatgc tgaacgtaag catgcaggat
    43321 cgccgcaccg tttgcagact gatcgaccag cgacgcgctg tctgaaccca gacggaacag
    43381 cgccaggtac agcagagtca gaccaacacc cgccatcagg ccagcccaga cggtataacg
    43441 ggtcagcaga cgcgcttcgg taacgccacg agaacgcgcc gcgttaacaa taacgatacc
    43501 aaacaccatt gcgcccagcg tatccatggt cagatagccg ttgacgaagc cgttagaaaa
    43561 cgcagcgttt tgataagcct cagtcgccgt gctgatagaa cccgccggcc agataattgc
    43621 ggcaacagac aggatgacca gcgcgataat tttcagcggc gcaaggaagt tgcccacggt
    43681 atccagcagc ttgcccggat agagcgaaac cagaataacg atagcgaaat agaccaggct
    43741 gtaaataaac agcggcagcg cggaatcacc cgtcagcggc gcaatgccca cttcaaaaga
    43801 aacggtagct gtacgcggcg tagcgaaaag cggccccacc gccaggtaac aaactgttgc
    43861 cagcagtacg ccagcgactt taccaattgg cgtgctgaga ctgtcaacac cgccgccaac
    43921 ttttgccagc gccactaccg ttaataccgg taggccaacg gcagtaatga ggaagccgaa
    43981 tgccgcagtc cagacgtgtt cgcctgcctg caagccgacc attggaggga aaataatgtt
    44041 acctgcgccg acgaacaacg caaatgtcat aaagcccaga gcgatgatat cgcgcgatct
    44101 taattgatgg gtcataaatc atactgcctg tggatgtggt gttgtaacgt taaatttttg
    44161 tgccaatccc tggggatgat acagcggtat atttaaacaa ttttagcggg aaatactccc
    44221 aaccaggcgc ggcgaagaat agtttttcga tttaccgcgt aaatgcagtc agtatgacag
    44281 cacctgaagc gcaatttaaa cgcttataac gttttaaagc aagaagggat ctaaaaacca
    44341 gaggtatata gcgataagaa atcaccaacc agtataaaac actatttcaa tgaaaaagac
    44401 atggctaatc atgcgaacaa aaaatcacca accattgtaa aatctaacgc ccgtatttcg
    44461 actgcaaaga aaataagcca gcatcggctg gcttatggca ataaaagatg acaaaggcga
    44521 attaatcgct gtttttggca attaaacgtt ccgggataac aaaactgaaa cgtgtacctt
    44581 ttcctactgt actctcaata ttcaggcgac tttcgtgatg attcaccgca tgtttcacga
    44641 tcgctaaccc taatccgcta ccaccggttt gccgggaacg cgctttatca acgcgataaa
    44701 aacgctcggt caggcgcgga atatgctccg gtgcaatgcc cggtccgtta tcttcaacac
    44761 taaattcggc accgtgcggc actcgctgcc agcgtacggt gatatgcgtg ccttccggcg
    44821 tatgattcac ggcgttatag accaggttcg aaatcgcact gcgtagctga tcttcattgc
    44881 ctgacacctt gaggccgtta tctatctcaa aggtaaatgt ctgttttttc tgactcagag
    44941 tctgagcctc gcgctcaacg acgcgcagca tcatcggcac atcaaccttt tcattgagca
    45001 aatgcgtcgg tgcggcttct attttcgaca gcgtcagcaa ttgcttcacc aacccttcca
    45061 tccgctgggt ctgctcgcgc atggtgtgca acgctttttc acgtaccgcg ccttccagcg
    45121 gttgctcatc catcatctcc aggtaaccct gtaacacggt caatggcgta cgtaactcat
    45181 ggctcacgtt ggcaaaaaag ttacgccgcg ccccttccag ttgatgcatt tgcgtgacat
    45241 ctcgcgccac catcagcaac tgtttgtggg tataaggcat gacgcgaatc tccagatgcc
    45301 gcccggtgtt gagcaccaga ttgagcgggc gagagaaatc acgcgttttc agatattgcg
    45361 taaactccgg gtaacgcagc aagttaagga tgttctgccc gttatcttcc ggccagcgca
    45421 agccaagaat ctgttgcgcc agaccgttgc accagaaaat accgccctct tccgtggtca
    45481 gcaccaccgc gtcgggcagc gactccgcgc cgctacgaaa gcgtttaatc agattgccca
    45541 gttcacggcg gcgtttttta tttcgcagct gcatctggtg taagccgtat agtagcggtt
    45601 cccagctacc acgccccggt ggcggggtca tactacgatc cacccacagc caccatgaaa
    45661 ggcgcaataa attccagaaa tgccagataa gcagtcctgt taccgatgcc agcaaaaacc
    45721 agggcaggta accaaaaaat gcacccagga tgaaagccag gaggcagcaa agtagcagct
    45781 ccagcaccag ccttttccac gacagccgtt ccagcacgta agatactcca gttaagaaat
    45841 cataagccct gctctacgtc cgatgaacaa ggccttaaaa gcgggttgaa aaacgatacc
    45901 ctgtaccgcg cacggtctgc accatgcggt catgcccgcc gggctccagt gctttacgca
    45961 ggcgacgaat gtggacatcg accgtacgat cttccacata cacgttggtt ccccagacgt
    46021 ggtttaacag ctgctcgcgg ctgtacacgc gctcaggatg cgtcataaaa aagtgcagca
    46081 gtttaaattc tgtcggcccc atctccagcg gttcttcgcc cgccatcacc cggtgagatg
    46141 tcgggtccag acttagcccc tgcatctcga tcacctcttc caccgccatt ggcgaaatac
    46201 ggcgcattac cgctttgatt cgcgccacca gctccttcgg agaaaacggc ttggtgatat
    46261 agtcatccgc accggtttca aggccgcgca cgcgatcttc ttcttcccct ctggcggtca
    46321 acattaccac tgggatatcc cgggtcatcg actcgcgctt gaggtgtttg atgaactgga
    46381 taccggagcc gccaggtaac atccagtcaa ggagaattaa atccggccag ggttcattca
    46441 gttgattcac agcactgtca taatcttccg cttcgaccgg ctgaaagcca ttttgttcga
    46501 gcacgaagca gaccatttcg cgaattggag cttcatcttc tacgaccaga atacgtctcg
    46561 ccatgatttg ccctgttgta atagataggt tgcgatcatt aatgcgacgt cattatgcgt
    46621 cagatttatg acagatttat gaaaagctcg tcgcacatat cttcaggtta ttgatttccg
    46681 tggcacagaa aaaagcaaat ggcacatctg tttgggtata atcgcgccca tgctttttcg
    46741 ccagggaacc gttatgcgca tccttcacac ctcagactgg catctcggcc agaacttcta
    46801 cagtaaaaac cgcgaagctg aacatcaggc ttttcttgac tggctgctgg agacagcaca
    46861 agcccatcag gtggatgcga ttattgttgc cggtgatgtt ttcgataccg gctcgccgcc
    46921 cagttacgcc cgtactttat ataaccgttt tgttgtcaat ttacagcaaa ctggctgtca
    46981 tctggtggta ctggcaggaa accatgactc ggtcgccacg ctgaatgaat cccgcgatat
    47041 catggcgttc ctcaatacta ccgtggtcgc cagcgtcgga catacgccgc aaatcttgcc
    47101 tcgtcgcgac gggacgccag gcgcagtgct gtgtcccata ccttttttac gcccgcgtga
    47161 cattattacc agccaggcgg ggcttaacgg tattgaaaaa cagcagcatt tgctggcggc
    47221 tattactgat tattatcaac aacactatgc cgatgcctgc aaactgcgcg gcgatcagcc
    47281 tctgcccatc atcgccactg gacatttaac gaccgtgggt gccagtaaaa gtgacgccgt
    47341 gcgtgacatt tatattggca cgctggacgc gtttccggca caaaactttc caccagccga
    47401 ctacatcgcg ctcgggcata ttcaccgcgc gcagattatt ggcggcatgg aacatgttcg
    47461 ctattgcggt tcccccattc cactgagttt tgatgaatgc ggtaagagta aatatgtcca
    47521 tctggtgaca ttttcaaacg gcaaattaga gagcgtggaa aacctgaacg taccggtaac
    47581 gcaacccatg gcggtgctga aaggcgatct ggcgtcgatt accgcacagc tggaacagtg
    47641 gcgcgatata tcacaggagc cgcctgtctg gctggatatc gaaatcacta ctgatgagta
    47701 tctgcatgat attcagcgca aaatccaggc attaaccgaa tcattgcctg tcgaagtatt
    47761 gctggtacgc cggagtcgtg aacagcgcga gcgtgtgtta gccagccaac agcgtgaaac
    47821 cctcagcgaa ctcagcgtcg aagaggtgtt caatcgccgt ctggcactgg aagaactgga
    47881 tgaatcacag cagcaacgtc tgcagcatct tttcgccacg acgttgcata gcctcgccgg
    47941 agaacacgaa gcatgaaaat tctcagcctg cgcctgaaaa acctgaactc attaaaaggc
    48001 gaatggaaga ttgatttcac ccgcgagccg ttcgccagca acgggctgtt tgctattacc
    48061 ggcccaaccg gtgcggggaa aaccaccctg ctggacgcca tttgtctggc gctgtatcac
    48121 gaaaccccgc gcctctctaa cgtttcacaa tcgcaaaatg atctcatgac ccgcgatacc
    48181 gccgaatgtc tggcggaagt ggagtttgaa gtcaaaggtg aagcgtatcg cgcgttctgg
    48241 agccagaatc gggcgcgtaa ccagccggac ggtaatttgc aggtgccacg cgtagagctg
    48301 gcgcgctgtg cagacggcaa aattctcgcc gacaaagtga aagataagct ggaactgacg
    48361 gcgacgttaa ccggactgga ttacgggcgc ttcacccgtt cgatgctgct ttcgcagggg
    48421 caatttgctg ccttcctgaa tgccaaaccc aaagaacgcg cagaattgct cgaggagtta
    48481 accggcactg aaatctacgg gcaaatctcg gcgatggttt ttgagcagca caaatcggcc
    48541 cgcacagagc tggagaagct gcaagcgcag gccagcggcg tcgcgttgct cacgccagaa
    48601 caagtgcaat cgctgacagc gagtttgcag gtacttactg acgaagaaaa acagttaatt
    48661 accgcgcagc agcaagaaca acaatcgcta aactggttaa cgcgtctgga cgaattgcag
    48721 caagaaggca gccgccgtca acaggccttg caacaggcgt tagccgaaga agaaaaagcg
    48781 caacctcaac tggcggcgct cagtctggca caaccggcac gaaatcttcg tccgcactgg
    48841 gaacgcatcg cagaatacag cacggcgctg gcgcatactc gccagcagat tgaagaagta
    48901 aatactcgct tacagagcac aatggcgctt cgtgcgagca ttcgccacca cgcggcgaag
    48961 cagtcagcag aattacagca gcagcaacaa agcctgaatg cctggttaca ggaacacgac
    49021 cgcttccgtc agtggaacaa cgaactggcg ggttggcgtg cgcagttctc ccaacaaacc
    49081 tgcgatcgcg agcatctgcg gcaatggcag caacagttaa ctcatgctga gcaaaaactt
    49141 aatgcgcttg cggcgatcac gttgacgtta accgccgatg aagttgctac cgccctggca
    49201 caacatgctg agcaacgccc actgcgtcag cgcctggtcg cactgcatgg gcagattgtt
    49261 ccccaacaaa aacgtctggc gcagttacag gtcgctatcc agaatgtcac tctggaacag
    49321 acgcaacgta atgccgcact taacgaaatg cgccatcgtt ataaagaaaa aacgcaacaa
    49381 cttgccgatg tgaaaaccat ttgcgagcag gaagcgcgca ttaaaacgct ggaagcccag
    49441 cgcgcacagt tacaggcggg tcagccttgt ccactttgtg gttccagcag ccacccggcg
    49501 gtcgaggcgt atcaggcgct ggaaccgggc gttaatcagg cccggctatt aacgctggaa
    49561 aaagaagtga aaaagctcgg cgaagaaggt gcggcgctac gtgggcaact ggatgcatta
    49621 acgaagcagc ttcagcgcga tgaaaacgaa gcgcaaagcc tccggcaaga tgagcaagca
    49681 cttactcaac aatggcaagc cgtcacggcc agcctcaata tcaccctgca gccacaggac
    49741 gatattcaac cgtggctaga tgcacaagat gagcatgaac gccagctgcg gttactcaac
    49801 caacggcatg aattacaagg gcagattgcc gcgcataacc agcaaattat ccagtatcaa
    49861 cagcaaattg aacaacgcca gcagcaactc ttaacggcat tggcgggtta tgcactgaca
    49921 ttgccacagg aagatgaaga agagagctgg ttggcgacgc gtcagcaaga agcgcagagc
    49981 tggcagcaac gccagaacga attaaccgcg ctgcaaaacc gtatgcagca gctgacgccg
    50041 attctggaaa cgttgccgca aagtgatgaa ctcccgcaca gcgaagaaac tgtggcgctg
    50101 gaaaactggc ggcaggttca tgaacaatgt ctcgcattac acagccagca gcagacgtta
    50161 cagcaacagg atgttctggc ggcgcaaagt ctgcaaaaag cccaggcgca gttttacacc
    50221 gcgctacagg cgagcgtctt tgacgatcag caggcgttcc ttgcggcgct catggatgaa
    50281 caaacactaa cgcagctgga acagctcaag cagaatctgg aaaaccagcg ccgtcaggcg
    50341 caaacgctgg tcactcagac agcagaaacg ctgacacagc atcagcaaca ccgacctggc
    50401 gggttgtctc tcactgtgac ggtggagcag attcagcaag agttagcgca aactcaccaa
    50461 aagttgcgtg aaaacaccac gagtcaaggc gagattcgcc agcaactgaa gcaggatgca
    50521 gataaccgtc agcaacaaca aaccttaatg cagcaaattg ctcaaatgac gcagcaggtt
    50581 gaggactggg gatatctgaa ttcgctaata ggttccaaag agggcgataa attccgcaag
    50641 tttgcccaag ggctgacgct ggataattta gtccatctcg ctaatcagca acttacccgg
    50701 ctgcacgggc gctatctgtt acagcgcaaa gccagcgagg cgctggaagt tgaggttgtt
    50761 gatacctggc aggctgatgc ggtacgcgat acccgtaccc tttccggcgg cgaaagtttc
    50821 ctcgtcagtc tggcgctggc actggcgctt tcagatctgg tcagccataa aacgcgtatt
    50881 gactcgctgt tccttgatga aggttttggc acactggata gcgaaacgct ggataccgcc
    50941 cttgatgcgc tggatgccct gaatgccagt ggcaaaacca tcggtgtgat tagccacgtt
    51001 gaggcgatga aagagcgtat tccggtgcag atcaaagtga agaagatcaa tggactgggc
    51061 tacagcaaac tggaaagtgc gtttgcagtg aaataactat tcagcaggat aatgaataca
    51121 gaggggcgaa ttatctcttg gcccgactgg tcgttatcct gcaagctatc actttattgg
    51181 ctacggtgat tggtagccgt tctggtggtt gtgatggtgg tatgaaaaaa gttattttat
    51241 ctttggctct gggcacgttt ggtttgggga tggccgaatt tggcattatg ggcgtgctca
    51301 cggagctggc gcataacgta ggaatttcga ttcctgccgc cgggcatatg atctcgtatt
    51361 atgcgctggg ggtggtggtc ggtgcgccta taatcgcgct tttttccagt cgctactcac
    51421 tcaaacatat cttgttgttt ctggtggcgt tgtgcgtcat tggcaacgcc atgttcacgc
    51481 tctcttcgtc ttacctgatg ctcgccattg gtcggctggt ttccggcttt ccgcatggtg
    51541 cattttttgg cgtcggcgcg atcgtgttat caaaaattat caaacccggc aaagtcaccg
    51601 ccgccgtggc agggatggtt tccgggatga cagtcgccaa tttgctgggc attccactgg
    51661 gaacgtattt aagtcaggaa tttagttggc gttacacctt tttgttgatc gctgttttta
    51721 atattgcggt gatggcatcg gtctattttt gggtgccgga tattcgcgac gaggcgaaag
    51781 gaaaactacg cgaacaattt cattttttac gcagccctgc cccgtggtta attttcgccg
    51841 ctacgatgtt tggcaacgca ggtgtgtttg cctggttcag ctacgtaaag ccatacatga
    51901 tgtttatttc cggtttttcg gaaacggcga tgacctttat tatgatgtta gttgggctag
    51961 ggatggtgct ggggaatatg ctaagtggca ggatttcagg acgttattca ccactgcgca
    52021 ttgcagcagt gactgacttt atcattgtac tggcactgct gatgctcttt ttcttcggcg
    52081 gtatgaaaac aacgtcgctt atttttgctt ttatttgttg cgcgggatta tttgccctat
    52141 cagcacctct acaaatatta ttactgcaaa atgccaaagg cggagagtta ttaggtgccg
    52201 caggtgggca aatagcgttt aacctcggta gcgccgtcgg cgcatattgc ggtggtatga
    52261 tgctgacgct ggggctggca tataattacg tggcgctgcc cgctgccctg ctttcgtttg
    52321 ctgcgatgtc gtcgttgctg ctgtatggtc gctataagcg ccagcaagcg gcggatagtt
    52381 cggtgctggc gaaaccactg gggtagatag cagtcgcagt gcgcatcgct tgttgccgga
    52441 tgcggcgtga acgccttatc cggcctacgg gactgttcat tttgtaggcc tgataagaca
    52501 cgccagtgtc gcatcaggca tcggagcact ggggggttgc cgccactgtg cgcgttattt
    52561 gttgtcggat gcggcgcgag cgccttatcc ggcctaccgg acggctcact ttgtaggcct
    52621 gataagacgc ggcaagcgtc gcatcaggca tgatacgcca attgcctggt ttttttactc
    52681 ttgtggccat aaccatgcag cgccgcgtac gccgctcgaa tcaccatgtt tcgccttgcg
    52741 taccggcgtt tcacattctc cgccgaagac aaattgttta atcaactgtg gaaccgtttg
    52801 atataaacgg tctacattgc tcattccgcc gcccaggaca atcacatccg gatcgagaat
    52861 attcacgaca tgtgccagcg attttgccag ccgcagctcg tagcgacgca atgccagttc
    52921 cgctaccgga tcgctttctt caaccaggcg gataatttca ctgcctttca gcgcatgtcc
    52981 gctcaaacga cgataatccg tcgcgaatcc cgtgcctgaa ataaaggttt caatacaacc
    53041 ttgtttaccg caataacaag ggacttcctc gcgataacgc agttcgtctt cgtccatcca
    53101 cggtagcgga ttgtgtcccc actcgcctgc cgtgccattg ccgccgatat gcgcccgccc
    53161 attgaatgcc acgcccgcgc cgcatcccgt gccgataatc acggcaaata ccgtctgcgc
    53221 tcccgctgcc gcgccatcta ccgcttctga aaccgccaga cagttggcgt catttgccag
    53281 ccgcacttcc cgctgcaacc gcgcgcttaa gtctttatcg aatggctgac cgttgagcca
    53341 ggttgaattg gcattcttca ccacgccggt gtagggcgaa atcgaaccag gaatgcccat
    53401 acctaccgtt ccgcgctgcc ctgttgcctg ctccgccata tcaaccaacg tggcgatcgt
    53461 ttcaatagtc tgccggtaat catcacgcgg cgtgggcaga cgatggcggt acaactgctc
    53521 ccctgcatcg cccaatgcaa tcacttcagt tttggtgccg cctaaatcga tacctatacg
    53581 cacggtactc tccttatttt tttcaatatc aatagcgtag agacggacaa ccggattggc
    53641 aatgcaaggc cgccgacaat tcgttatcat gcccgctaaa tttaacgaca aggccgtgga
    53701 aattatcatg ctgtggttca aaaatttaat ggtttaccgt cttagccgcg agatttcgct
    53761 gcgtgcagaa gagatggaaa aacagctagc ctcgatggca tttaccccat gcggcagcca
    53821 ggacatggcg aagatgggct gggtacctcc gatgggatcg cacagcgatg cgttaacgca
    53881 cgttgccaat ggtcaaattg ttatctgtgc gcgcaaagaa gaaaaaatcc tcccgtcccc
    53941 ggtgattaaa caggcgctgg aagcgaaaat cgccaaactg gaagcggaac aggcgcgtaa
    54001 gctgaagaaa accgaaaaag attcgctgaa agacgaagtg ctgcactctc tgctgccgcg
    54061 tgctttcagc cgttttagcc agacaatgat gtggatcgac acggttaacg gtttgattat
    54121 ggtggactgc gccagcgcca aaaaagcgga agatacgctg gcactgctgc gtaaaagcct
    54181 ggggtcgtta ccggttgtac cattgagcat ggaaaacccg attgaactga cgctgaccga
    54241 atgggtacgt tccggcagca cggcacaggg cttccagctg cttgatgaag ccgagctgaa
    54301 atcgttgctg gaagatggcg gcgtgatccg cgcgaagaaa caagatctga ccagcgaaga
    54361 gatcaccaac cacattgaag ccggaaaagt ggtgactaaa ctggcgctcg actggcagca
    54421 gcgcattcag tttgtgatgt gcgacgatgg ttcgctcaag cgtctgaagt tctgcgacga
    54481 gctgcgcgat caaaacgaag atatcgaccg tgaagatttc gcccagcgtt ttgatgccga
    54541 tttcatcctg atgactggtg aactggcagc gttaattcaa aacctgattg aaggattagg
    54601 tggcgaagca caacgttaat tgctgattta cctttaatgc cggatgcgac gcctgccgcg
    54661 tcttatccgg cgtacgaagc cacaccagga atataatcgt tagctacagc gggcaataat
    54721 tcttagcgca gcaatattat gcgttttatg ctgtaacttg ctccaggggc gttgtgtcat
    54781 tgtttttcct caagccggtg acgcgcttca ctgcgaattt tccgcgttgc cccgtttttc
    54841 actaaatcac gtaacagcga aagatcgggt tgttcgacaa ataagagtaa tgatggctcc
    54901 tcttttaacc acgccgtttt cacatcggca gccagctgtg aattattaat agccagcgac
    54961 cgctcctgag gctcagtcaa tgtcgttaac aacaacaccg gggtatgtgc gttgtgcaat
    55021 gccgtttggc gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct
    55081 gatttatctt ttatcgtcgc cagttccgca acaatatctg gagatcttcg ctgatattta
    55141 tatcaacctt gatgaattat gaatatatcg gaaatcgaaa aaatcccagc aaaggaaata
    55201 ttattatggg ttaaagagag gacatttcag aaaaatgccc tcccgttcaa ggggaaggcg
    55261 aggaattaca gatagcggca cagataagag gtgggttcgg caacttgcag atgaaactcg
    55321 ctgtgaccgg gaacattaaa caccgaaccg gcttcataca cttgccagtc ggtcgcgtca
    55381 ggcagtaaca cattcagcgc gccactgatt accgtcatct cttcaggctc agcggtgctg
    55441 aaggtgtatt caccttcaac cataacaccc acgctggcgc gaccagtgct gctgctggaa
    55501 aagccgatcg atttcacttt gccggaaaag tactcattac tttgaagcat aaactggccc
    55561 tgtctggtag ttgaacatct acttcactat aggggccaga ggcgctggct gtcacgcaaa
    55621 attacatcag taattccgca gccagccgtg caatcaatac gttagagagc aagacaggaa
    55681 catcgagctg tttttgcagt aaatcgcgat gacgctggtt aaatcccaaa caatccagca
    55741 tgatgacatc tgcacctttt gccagtaatt ctttcccggc atcaatgatt ttttgttccg
    55801 aatcatgaat ggggttaccc aatgaaaata ctggcggttt ctgcaaaatt tgccattttt
    55861 gcgcctgaac ggtcagcaac tcctcaaccg gtacgataac ccccacctga tgatcttcaa
    55921 caatagagaa aaccagcggt ggcaatatcc tcgacggctc aagaaagatc gtattacgcg
    55981 cggtcatact actaatgttt gctgtactca ttaatataat gacgtcgtaa ccctgattat
    56041 cgagcacttc aaccacacct tgcaggtctc gctccacttt gcgacgcgaa acatgggcca
    56101 gatggttgtc atttaataat gtgagaatgg tgtcttcgcc tgcttctggc gcgtactccg
    56161 ccatcacttc ttcgcggctt aacttcccca gcaggctatg atgggaaata ttatcttcgt
    56221 caatgtattc tgtcaggagc ggcaaaactt cctgcatagg tacaatgccg atagtcagga
    56281 tcgccaacga cgcactcatt gttcttccac cttactttta tttcgtcgtt aattaccggg
    56341 caagtgttaa gcaccactgc tgacacttac ttgtgctaca aatgacaaaa agcgtagcag
    56401 cagacctgca ctgcataatg taaagattcc aaatgattcc agtaatggat tgttattgtt
    56461 taatattcta attatttgaa aaacatgaat tgtgaaaaat gtgacgcaga tcccttgtgg
    56521 cggcactcag aatcccttcc gagcagtctg atgcggtcac tctttgtcag ggtcctcata
    56581 gcgttttttc gcatccatcg cttcctgagc ggtcggatgt tcactgatca acgagtctgg
    56641 tttaggatga tcggctctga gttggtacca ggttaccgtc tgtcctggct ttcctttatc
    56701 aatcgtcact atatatgctt cacgaggata aggcggtttc gttggcatcg ttcttcctta
    56761 tttcacggga tgaacgttaa gtataggcgc tcgaaaatca acaattgatc gtctgtgcca
    56821 gagcgctgcg aatttcagaa atcacctggc tgggttcgtt tgttgcgtcg atgataatat
    56881 gcgcaacttc gcgatatagc gcatcgcgtt cttccagcac ttcctgaact tcttcgctca
    56941 gtggttttcc cgttaaggtt ggccgtaaat cttcttccgg tgcagcttgc agtcggttaa
    57001 ccaggactga tactggcgca cacaaataaa ccacgatccc gttattttgc atgaagtgac
    57061 gattaaattc cgtcagaata atgccgccgc ctgtagcgat aacggtggat ggcgcagtta
    57121 ccgcttccag cgccgccgtt tctctggcgc ggaatcccgc ccactcttcc ctttcgacga
    57181 tctccgcgac cgtcatattg agctgtgatt gcaaccacag gtcggtatcg acaaaccgac
    57241 ggttaagcga atcggcaagg gccattccga ccgttgtttt accacagccc cgaggcccga
    57301 tcagaaaaag aggttgtgtc atcgtgggtt ttccccaata ggtcgcaatg cggcgaaagc
    57361 cggtgtcatg agaatagcga tcataccatc aaactagtac aatttcgatt gtaaagaaaa
    57421 aattccactt aaggtggaaa tctcaataca tcccttacta taccaataaa tattcaagaa
    57481 tgaagtgtaa ataataaatt acatttagcc acgactacgt tgcacttcca gccaccactt
    57541 ctccagctcc gcagcaaaag cctggcggtc acgttgtgaa agactatctg gtcctccggt
    57601 ctggatccca ctggcacgta aggtatccat aaaatcgcgc atcgtcaggc gctcacgaat
    57661 ggtcgctggc gtgtaacgtt cgccgcgcgg attaagcgcc gccgcgcctt tctcgatggc
    57721 ttcagcagcc aaaggtatat ctgcggtgat caccaaatcg cccgcttcac actgccgaac
    57781 aatttcgtta tcagcaacgt cgaaacctgc cgcgacgcgc agcgtacgaa taaatcgtga
    57841 tggcggcacg cgtaaactct ggtttgctac cagtaccagc ggcatctgca tacgttccgc
    57901 cgcgcgatac aaaatctctt taattacatt gggacacgcg tcggcatcca cccaaattgt
    57961 cataaagtca tcctttgttg ggtaatcctc tattgtgtcg cgcttttgcc ttccggcata
    58021 gttctgttta tgcttctgcc agcgattatc aaaacaatga atttcacggc aggagtgagg
    58081 caatggaaaa gaaaatcggt tttattggct gcggcaatat gggaaaagcc attctcggcg
    58141 gtctgattgc cagcggtcag gtgcttccag ggcaaatctg ggtatatacc ccctccccgg
    58201 ataaagtcac cgccctgcat gaccagttcg gcatcaacgc cgcagaatcg gcgcaagaag
    58261 tagcgcaaat cgccgacatc atttttgccg ccgttaaacc aggcatcatg attaaagtgc
    58321 ttagcgaaat cacctccagc ctgaataaag actctctggt cgtttctatt gctgcaggta
    58381 tcacgctcga ccagcttgcc cgcgcgctgg gccatgaccg gaaaattatc cgcgccatgc
    58441 cgaacactcc cgcgctggtt aatgccggga tgacctccgt aacgccaaac gcgctggtaa
    58501 ccccagaaga taccgctgat gtgctgaata ttttccgctg ctttggcgaa gcggaagtga
    58561 ttgctgagcc gatgatccac ccggtggtcg gtgtgagcgg ttcttcgcca gcctacgtct
    58621 ttatgtttat cgaagcgatg gccgacgccg ccgtgctggg cgggatgcca cgcgcccagg
    58681 cgtataaatt tgccgctcag gcggtaatgg gctccgcaaa aatggtgctg gaaacgggag
    58741 aacatccggg aacactgaaa gatatggtct gctcaccggg aggcaccacc attgaggcgg
    58801 tacgcgtact ggaagagaaa ggcttccgtg ccgcagtgat cgaagcgatg acgaagtgta
    58861 tggaaaaatc agaaaaactc agcaaatcct gatggctttt gccggacgtc aggccgccac
    58921 ttcggtgcgg ttacgtccgg ctttctttgc tttgtagagc gccaaatctg ccgatttcaa
    58981 ccactcgcga tagtgactca tttgtggatt cagcggcgca acccccacac taatccgtaa
    59041 agttacctgt ggcgtattcg gcaagcgtaa cgtgtttagc ccttcatgca cccgtaacat
    59101 ggcggtgatg gcgctctcag ctggcgtacc ggacatgatt actgcaaact catcgccgcc
    59161 aaaccgacca atcacatcgc taccgcgcag ggtaatttgt aactgtcggg taagcgccac
    59221 aatcgcttca tcacccacat catggcccca ggtatcgttg atgctcttga aatggtcgat
    59281 atcgataatc agtaacgttg catcgcgatt atgccgccga cagttatcaa attcattgcg
    59341 taacatagtt tcccaatgac gtcggttata cacgcccgtc ataccgtcgc gggtactcat
    59401 gacctgcaac ctgcgtttat gttccgccag tttagtcgcc gtctggtagc tgacccagcc
    59461 aaacagcaga ggataaatga caataatggg aagggagagc caccattcca gcggtgcgct
    59521 attgaacgac acggtgatgc ccgtcagctc gagggtgaca aggcaggaga ccaccatcaa
    59581 caccagaccc gcgacaaaca gacgggggcc gcctgccccc atcaaattca gacacataat
    59641 catcaacatc gcggtggaag gcagtacgtt tacgcccatt acgcctaccc acattcccgc
    59701 taatactgca tcggttttta agttgtaaat ttcccggcta agcggatcga cggccctgct
    59761 cgctatttgc caggctaaat gcggccagac gaacgcccag ccgaccaaca acagccacca
    59821 ccagcccggc gacggatgcg aaaccagcgt tgaagcaatc ggtaagaaca tgccagccag
    59881 gccaaccgcg cggggtagtc tgacgcgacg ggcaaagcgc agcccggacc gctgatgttc
    59941 gttttgagga gttaaaggag gttcctcccc atgcgccgcc gcttttttga aaaagttttc
    60001 atcattcatt atttttggga acattcagaa acaattttcc caaattatag agacggggct
    60061 aaaggcagaa gtatgaaatt tcgggtgagc cattttcgta gctcacccgt tagcactatc
    60121 aggtatcagg cggctttctt gaggcaggca ctcataaact tattacgatc atcccctttc
    60181 agagattgtt gtgttgcttg attattacat tcgcgcatct tttgctgctg tggcgtcaaa
    60241 cttttttcac caggcgcaga tttgctgttc ttcaggcaat cactcatgta ggtcttacga
    60301 gcatcccctt tcaacgcctg cgccgtcgcc tgctgattac aagaggtcat acgctgttgt
    60361 tgtggggtta aggttctctc ggcagcgccg acggtggtta aaaaaaccag accgaaaagc
    60421 aaggtaacca gtaatgtaat tttcatcgca ccatccctct tcatgtttta accatgagcg
    60481 tatgcgcccg tgatctgtca ttaagtctgg ttgctaacag caaaaaaacc acccggcagc
    60541 gaaaattcac tgccgggcgt ggttttattt cagccccagg gcggctttca tggtgtagaa
    60601 gagatcggtc tggtcggtca gtccaacaac attggcggca tgagggccat acgccgcaat
    60661 acgcaactga ctgccggtat gttcttgtga atcctcttcg gagttcccgt aactcatcac
    60721 catcactgcg ccatctttgg tatttagcgc ctgggtgagg cccggagctt tggtatccgg
    60781 cgcaactatc tggctggcat gggcgtgatc agcggtgact atgaccaacg tgttaccatc
    60841 ctttttagcg aattccagcg cccgttgtac ggcttcatcg agatcgaccg tctcgccaat
    60901 ttgcccacaa ggattcgcag cgtgatcctg tttatcgatt gacgcacctt caacttgcag
    60961 gaaaaagcct ttctcatttt tactcaacaa ttcaatggct ttgtcggtca tctgcgccag
    61021 ggtcggtaca ctgtcattac gttgcggatt aggcgtacag gtgactgcgg gcttatctat
    61081 attaccgtgg tacgctgctt tcggtccttg ccagcgcact ggcatattgc cgtcagcaaa
    61141 cagtcctaat aggggttttt gctgattcgc ttccgtcacc gaattcagtg aggcagcatc
    61201 gctcaccaac tgataaccac gcgcctgtgc ctgttcacgc agcgtttttc cctgccattc
    61261 accggcggtt gccgtttcag caaaggtttt tgcgccgccg ccaagcgtaa catcggcacg
    61321 ggcgttaagc agctgttcgg taatcgatcc ttttccgcct ttttccagag cgttacccgg
    61381 acatttttca ctggtcgcac tcggaccgta gcatttgcgc gaggtcacat gcgccaccag
    61441 cgcagcgggc gtggcatcct gcaactctgc ggtagaaacg ttaccggtcg ccagacctgc
    61501 ggcttttgcc atttccagaa tcgttgggtg atctttttcg tgaatatcga cgcccagcgc
    61561 gccgttatag gttttgacac cagttgacca ggcggttgct gatgcagccg agtcggtgac
    61621 gtagtccggt ttgccggttt ttttattcag cgcatagtga gtgtattgcc cggtaagcgg
    61681 taaggcatcg atacctttaa aaaagccgcc cgcaccttcg gcataattgc gtgcggcagt
    61741 aatttccgag tcccccatcc catcgccaat cagcaaaata atattttttg caggtttatc
    61801 gctaagagaa tcacgcagag cggcggtctg atcacccgtt aagcggcgag caccgccggg
    61861 tgcagtaata tcgccctgag cagcccggtt ttccagaaca ggcatttctg gtgtccgggc
    61921 ttttgtcaca ggggtaaaca gtaacagtaa gagtgccagt gcaatagtgc tttgtttcac
    61981 tttattttct ccatgtacaa atacattaaa aaataaaaac aaagcgacta taagtctcgg
    62041 ccgtgacaac tttatgacag ctgttgaaaa gattaacttt ttactgacga ggatgcctca
    62101 ataacttctt tacgtaatcg cgcagcagct ccgtatcgtc gtcaggaatg ctggcatcgg
    62161 gctttacctc gtacagcgcc ccctctacct gatcaatcaa ccgctgttga tcattttgcg
    62221 ccatattgcg aagcattgcc gtgacgataa tctccaaagc ttctacctgc gcacacagtt
    62281 ctttcgactc ttcttctttt tgggcaagct taaataacaa ctcagcaatg agatttttca
    62341 tgtatgtatt tccttatcca aagtatggag aagttaacat tatgtttcac aacttcatag
    62401 ttgtctggaa gtattatttt tatgacaaag gaaatacttt gcgcaaatat ttttttgttt
    62461 gattaccagc gaaacgtttc tcttattcaa at
//
LOCUS       DAZFFE010000014        59500 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFFE010000014 DAZFFE010000000
VERSION     DAZFFE010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.014"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..183)
                     /locus_tag="ACHJ69_001079"
                     /pseudo
     CDS             complement(1..183)
                     /locus_tag="ACHJ69_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(483..4340)
                     /gene="sigA"
                     /locus_tag="ACHJ69_001080"
     CDS             complement(483..4340)
                     /gene="sigA"
                     /locus_tag="ACHJ69_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFO5014121.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYALFNNDRVNNLKNNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVSGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKTAQGNNLKTGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQKHNNNTNLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGS
                     NNCLIPLLCQKDYSAAIRDQESTVNKRYNTEYKSNNQVASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQVHSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQDKEDNLDKTTHIPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNMSPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLIGKYVHHDNEYSATFAGLGTKDYSSHSLYAGAEAGYRYHV
                     TEDAWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPVIGRAGVDVGKSFSGKDW
                     KVTARAGVGYQFDLLNNGETVLRDASGEKRIKGEKDGRMLMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAVNASFRYSF"
     gene            complement(4702..5904)
                     /locus_tag="ACHJ69_001081"
     CDS             complement(4702..5904)
                     /locus_tag="ACHJ69_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFO5014122.1"
                     /translation="MMMKKHITRTLVASAVLFSFNSAAATSYSEARNDAMGGTGVASS
                     HYGVAPLANPALLTKAGPDDDFSLLLPSVGAQLSDPDNITDNADRISDDWKAFDRAID
                     SNHGVPEAAARLKERLRDFRHTHAAAQLGVSAVAALPGDRLSAALMVKSHGTVSVDGK
                     VSDADLTYLEEVANSTGQEVDKSRLTSQAFARAALITDVGIALATELETAGQKWSLGF
                     TPKFQRVDLFNYNVLVRNYDSSAFKGDRYHNTKNGINADIGASTDLDDNWTLGLVAQN
                     LIPRSIETKEVNGITETFRIRPQVTAGVSWHNAMFTTAFDVDLTPASGFTSDSNRQFA
                     AIGAEFNAWKWAQLRAGYRQNLAGNDGSAFTAGVGISPFDVVHLDVAGLIGTDNTYGA
                     VAQFQFTF"
     gene            complement(5994..6815)
                     /locus_tag="ACHJ69_001082"
     CDS             complement(5994..6815)
                     /locus_tag="ACHJ69_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFO5014123.1"
                     /translation="MKPNSTGIAARMILSLDRARICEALLNNRQLQPTPLQVRYPQGV
                     REALSIMSEQLSLSVSDLTRILVEEALSEMFLPADNSVRRLHDRMEYLMQVHELSPVT
                     MATLLAPWNIRPAVFREPDRLTDYLSGEILASLADWFYLSPEWLNGRVHYPLCHPGDW
                     PETQDAFCRLISEDDNIDIILWHGFPFAGEHAQEYCGVLLRQKKKINNAIIYPVLSLY
                     PTGMNKKKEGWFQMARKMSPDIPVRAVTLSPAQAEYLVTGKILPAELFRIPLSPW"
     gene            complement(7376..8500)
                     /locus_tag="ACHJ69_001083"
     CDS             complement(7376..8500)
                     /locus_tag="ACHJ69_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO5014124.1"
                     /translation="MFRKIKIRKMTLNRALDKYLKTVSIHKKGHLQEFYRVNVIKRHP
                     MAERYMDEITTVDIATYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIASVEWGT
                     CRMNPVELVRKPKISSGRDRRLTSGEERRLSRYFRDKNQQLYVIFHLALETAMRQGEI
                     LTLRWEHLDLQHGVAHLPETKNGLPRDVPLSRKARNYLQILPQQINGNVFSYTSSGFK
                     SAWRTALLDLKIENLHFHDLRHEAISRFFELGTLNVMEVAAISGHHSLNMLKRYTHLR
                     AYQLVSKLDAKRKQTCKIAPYFVPYPATVGNRNGLFIVTLHDFDLETRAETRELAISH
                     ASVLLLRTLAQAAQRGERVPTPGELPANIDARVMICPLTS"
     gene            complement(8825..10004)
                     /gene="pilV"
                     /locus_tag="ACHJ69_001084"
                     /pseudo
     CDS             complement(8825..10004)
                     /gene="pilV"
                     /locus_tag="ACHJ69_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672434.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
     gene            complement(10001..10651)
                     /locus_tag="ACHJ69_001085"
     CDS             complement(10001..10651)
                     /locus_tag="ACHJ69_001085"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFO5014125.1"
                     /translation="MSELLPICIILIVLAAILYLAYSAATVYLSQVHNTRPHNAAVCI
                     CCLLAVLSICLNGAYGVDATGLLFLSILTGLLTVMTLTDIAVCRLPRIFTLSLIILGA
                     AFRYSQDSLAHALLNASLWFGMIYLLRRIFLAVKGTEALGLGDVFLIAGIAMWTQPQH
                     TPLIITAAASGAFLFILIFCRRRRQQALPFAPFLCASLYVLTLFPDSVIHTSEIFT"
     gene            complement(10664..11140)
                     /locus_tag="ACHJ69_001086"
     CDS             complement(10664..11140)
                     /locus_tag="ACHJ69_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO5014126.1"
                     /translation="MKWLYFSVLILFSHTSIASVMNNNHLDSCFIQAGKRYLIAPELL
                     KTIAQQESGLVATAINHNKNKSGKIISTDYGIMQINSSHIPELKKLGVIKDKDELLKN
                     PCLNIHTGAWILARHFQRCGVNWECLGSYNAGFSKNNTARRMIYARIIHNRYFRKD"
     gene            complement(11199..11732)
                     /locus_tag="ACHJ69_001087"
     CDS             complement(11199..11732)
                     /locus_tag="ACHJ69_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFO5014127.1"
                     /translation="MNFRARLFYYFSLKKEEEDKGLTLMEIAGALIIIGIVVGLAISQ
                     MSSTMSSSDNQRELKNLQTIATKMKQYKFQGQYTSSDYIKTLTQSGSLPADMIAGGNK
                     AKNAWGGDVTIKATTDKYGYTITSNNVPKENCVELINSLRSSSMFTKIMNTTPATVDP
                     VTVCSKDKNNITLETNS"
     gene            complement(11784..12884)
                     /locus_tag="ACHJ69_001088"
     CDS             complement(11784..12884)
                     /locus_tag="ACHJ69_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFO5014128.1"
                     /translation="MKYLEKLHSTDIELTALTVWIARKTFALNRVEFYEALGGMIRDG
                     TPSLRALEFLCEIETDFGEKKGTSGMYFLAKECIASIKASGMLSGALFTWVPPEEATL
                     IRNGEERGNIADALFQVARTVKAQREMTSSLIAVCLYPLLLLSLCVVNMYNAHTRIIP
                     IVSAFATEDKWSFQMKVLAGMSEFIIGYGLWLCGAIIALTVLIRFSFSRYTGPGRRML
                     DRVPPWSLYQTLHGVNYLFSISSMLQINIPLSQALLRMEKGAEKNKWLKERIQAIRKH
                     VLSGQNLATAMRNSGYDFPSRVCINKLLLNSEREDNVAAMAVYADQWLEEAKKRVKRS
                     GLIITGISGGFVFFFILNMISAIYSISDMLKQ"
     gene            complement(12886..14406)
                     /locus_tag="ACHJ69_001089"
     CDS             complement(12886..14406)
                     /locus_tag="ACHJ69_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFO5014129.1"
                     /translation="MPETIVLPPEDIRDFVAIQHDADGKIRVKVVEAKKHITSVQRYL
                     TLLRSRYPGKISKFDFTTLDVIATLNSNNTERAPGVLSDGGINTVQRKVLDYIRKAAQ
                     LDASDLHITPGRDNTDFTYVEARVHGELEVLDILRKEEGLELLGATYSGMTDVIKGTQ
                     FDPGVPQDARLSEQYLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSLQIPTFSMLGYH
                     PDQELTLRRILQRPEGIITLSGPTGSGKSTTLRTASAAYLEQYGFNNTGGILLPRRRL
                     FTIESPPEGRIPGAIQTAVMDSAQGWVDSIKSALRLDPDGILNGEIRDHDSAITAIKA
                     AMTGHLMLTTIHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQLICPHCRRP
                     WHEVATDRTDDERRLVESVCNPDQVYLRNHEGCPHCWRGVTGRTVIAEVISPDARFFQ
                     LYREKGRIEARTYWHRELGGMTRNQHLLGKINSGQVDPLAAHYISPVDEDSYTLLH"
     gene            complement(14416..14877)
                     /gene="pilP"
                     /locus_tag="ACHJ69_001090"
     CDS             complement(14416..14877)
                     /gene="pilP"
                     /locus_tag="ACHJ69_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFO5014130.1"
                     /translation="MHDKTKALLIAALLLPAMAVAGNENDASQNKQLQIEQIQEETAL
                     YEAMLAREKIRHELRTLEQGISPVSGSVTGHQPENAAGTPSGSQALSSPRLKEIFGIQ
                     PQLSARVILPGGTETDVRAGQALPGTLFMVEQITPDSVILEYNGNRQTLRP"
     gene            complement(14867..16162)
                     /gene="pilO2"
                     /locus_tag="ACHJ69_001091"
     CDS             complement(14867..16162)
                     /gene="pilO2"
                     /locus_tag="ACHJ69_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFO5014131.1"
                     /translation="MQKKIRPVSLISGKQAFLAGLDWKNIPPGYRNARRFARSQGAEL
                     YLSCHNHDGENGDEMMIALLSRQDADIPRNIRQCLSLAMLIRPLLKSGGYAIFIIGND
                     ENDDAELTCAFVSVINGILVNDVTGTPTEITEARNTFLMLNAEPENGWVRYEPDGFSP
                     DSRCIPLPLSNLISTRKHPAEARLLPVSRGPQLMTALLIITLLGASWYGWQRYEIWQA
                     EKAEREESARKSAEARITPPWTGQPETGEFIRQCSTTWNQTQLSAAGWRYTLAECSSD
                     GNSGNLRSSYTNTAGTTVTAFIRRITELTDTRPFIVMPEGNSGGVALPVTFRLPDNPV
                     PVDSLPETRDLQERLTTLAQSVQLQIDWPEVNNSFTDENGNIIQPPWKEYDLQIQTLL
                     PANQLAERFSEPSVRFLSVTRQLENGRFRYQFTGKYYAR"
     gene            complement(16186..17787)
                     /locus_tag="ACHJ69_001092"
     CDS             complement(16186..17787)
                     /locus_tag="ACHJ69_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFO5014132.1"
                     /translation="MKKFFCAVTALSVVLSGCSSFERISATEKKASDEITRAERIAGE
                     LRKQRAVVRETNQQWINTTPLPEKTVTRAIPDCPVVINTREAITLHQIAQRISETCHI
                     PVQIMSDVWTLLGTGENRTQRISGDIPPPDTSGMQPLSTVGLSSAAPSTASLPELNVQ
                     GLPALLDTVSSRFGITWRYVKGKIEFHWLDTRTFPVTYMDSQVAYNARVVSGTMSSTG
                     SSGGSSGSSLSGDASNTQTTAVDMKSTLYEDTKNAVNSMLTPGIGRMVLSTGFLTVTD
                     TPQVLDTVKNFMEERNREMRRQVVLNIEILSIKKTSKEQAGIDWNAVFSDGHLGIDLT
                     SAFTNAADEVVTSGVSIIDGKLTGSKAFLKALSSQGKVSVVTQNSAVTKNLTPVPMQV
                     ASQQGYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFIQPGSHRLQLLFSMSLSD
                     KPVFEVFESGGSRAQTPSVELKTINQTIDLKSDQTVILSGFQQFSRKSLRQGVGTPSF
                     FGLGGGVDSADDDTILVVLITPHVI"
     gene            complement(17996..18430)
                     /gene="pilM"
                     /locus_tag="ACHJ69_001093"
     CDS             complement(17996..18430)
                     /gene="pilM"
                     /locus_tag="ACHJ69_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFO5014133.1"
                     /translation="MGWLMPLIMALFIIGNWFHAGNTERAIKNTDEQQRQQQVYEILR
                     LTNAINDWKYHHPGDIREGKMLTVNETGFAPVYGTKHIISGQRTFVWLPFQPGLISAP
                     NEVSRNSALIAQVRDRKLLDNQGNNIGVPPSVIPENAIVLIN"
     gene            complement(18431..19102)
                     /locus_tag="ACHJ69_001094"
     CDS             complement(18431..19102)
                     /locus_tag="ACHJ69_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001023520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin co-regulated pilus biosynthesis Q family
                     protein"
                     /protein_id="HFO5014134.1"
                     /translation="MNILKYLSISLFAFSFSYTATASTQISSSHSHAEKRAIGTLKFI
                     RADGQRPFGQAMKQLLPTGWRTEIARDVRLPARISWSGNDQWNHVIEKTLPRYSLKAT
                     FDTSGKRVLIQQAEKAVKSGLFSAPVTGVKKTETRHIEQGMTLRTGLEKWAAAEPCAT
                     GKWKVAWKTPVNYSIDARLVFSGNFMDALNSTFALYQQAEKPLYASVNKNQCVLIITD
                     IPGDS"
     gene            complement(19602..19832)
                     /locus_tag="ACHJ69_001095"
     CDS             complement(19602..19832)
                     /locus_tag="ACHJ69_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO5014135.1"
                     /translation="MPSAQATPVPYKYCIQATIEKPGGTPVSWIRFQNTPMTKQECEK
                     LFSTEKEAGKSFTVKVGVTQFSCKENPPAPGE"
     gene            20372..21880
                     /locus_tag="ACHJ69_001096"
     CDS             20372..21880
                     /locus_tag="ACHJ69_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFO5014136.1"
                     /translation="MPHKPTEEQTAIIHWQGRKLVVNAFAGTGKTSTLVSYAMACPES
                     RMLYLAYNRAIRDEAERRFPFNVECKTSHQLAFARFGRHYQKRLVPGLFVTDVARKLN
                     TRYWALARVAMTGLNQFICSADATPGRQHLPDKDEMKGVAPDEALRAVRTLWQAMSDP
                     DGTFPVTHDTYLKLFQLSGPDLSRRWDTILFDEAQDANPVTSALVLGQRCRIVLVGDR
                     YQQIYRFRGADDALSHPSLAGADRLWLTQSFRFGPAVAAMANLLLKRAGESREVSGCG
                     GDDEVLTMQEMRERMKRGKFGGHRAVLNRTVAGVIGTALMAAMAGRKVYWVGGIEGYR
                     TEALEDLYWFQMDMPARMHSDRLRRDYRNFDEYRHIAKVTKDVEMSQSIRLLELCFPL
                     PKKLELLREHTVTNEREADITVSTAHRSKGLEWERVVLGDDFQDIADPLMSEQERRDE
                     TNLLYVAVTRARRTLVLNELMQQLAEEKSRTKEKTTSPHQRGTEKAVEGKDV"
     gene            21873..23483
                     /locus_tag="ACHJ69_001097"
     CDS             21873..23483
                     /locus_tag="ACHJ69_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HFO5014137.1"
                     /translation="MFKLRKFFTSGSAVEIPDDGRSVPGTPEGFYAPLTTDSLLMPKN
                     RAQCLKQIRDNNMMPKPLCEAMCCAPVYSLLLSTQNVPAAREGRWSHAGGFGDLTLLH
                     TSYAVRLARAHMFPPGASAEEQAAQGAAWHAVIFWSALFYHLPLLAHLEGKLFSGRGW
                     QPGISVPDEAYRFRFRQLPPQGTEAQQLAAVIAGGLLPEGAAAWLATVPGALQNLAGA
                     FWHQHPEMALIRDVLQEAARQAESPLLNGGIVATASHIASAEVFTGEVVVSGKTESTV
                     ASAVSGEEAAAEGNTVSGNAAESVQEQPEMRQQQAEKPTTPDSSEGSEDDAGCETESI
                     PTDEDTAMLVSMFAEEARAPEKGEDDSPSGEVVAPVSSTGEISAAGHGEICQSYQDAE
                     QQEYCEAESDAVNPDLTGERFLEWLKDGLKNGSLSFNNKGDKIHVVAGFVFIPVPGIF
                     YDYLKARQLPGEKREVLQTAFERLNIHKRKDNQRFYHARIAEAPGKTHGYKILKGYLI
                     KGRGLMDTGDLNDSPNFFFSERRRSVFW"
     gene            23514..24509
                     /locus_tag="ACHJ69_001098"
     CDS             23514..24509
                     /locus_tag="ACHJ69_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5014138.1"
                     /translation="MKLSHADMRWNELMDEYFFCRSVRVATEWSYRKVLNGFRKFVGE
                     TLLPEDIRQQHVREWRREVLKKQNRSTHTWNNKVRHMRAIFNFACSSTLLNLSENPFD
                     GMNVRQEKKRKKTLTKKQVKRILLILEQFHEEEKRSDGVHWRSCAIRPAWYWMIVVNT
                     LRFTGMRLNQLLHLRLRDVNLDERWIDLCSEGSKTHHEWRIPMVSHLVPGMRHLIDEA
                     MKRGAGPEDYLFHYHRYVALMSEAPFVTGLPELQPVKGFFRRLTKECGFPVSPHRFRH
                     TLASTLMSAPERNLYMVKTMLGHRNVSTTLEYVEIDLKGTARVLEKELAELTDVF"
     gene            complement(24708..24783)
                     /locus_tag="ACHJ69_001099"
     tRNA            complement(24708..24783)
                     /locus_tag="ACHJ69_001099"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24748..24750),aa:Phe,seq:gaa)
     gene            complement(24889..25596)
                     /gene="yqgA"
                     /locus_tag="ACHJ69_001100"
     CDS             complement(24889..25596)
                     /gene="yqgA"
                     /locus_tag="ACHJ69_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO5014139.1"
                     /translation="MVIGPFINASAVLLGGVFGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTVVAKAHNLFHHSRKKPAHESF
                     IQNFVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            25749..25832
                     /locus_tag="ACHJ69_001101"
     CDS             25749..25832
                     /locus_tag="ACHJ69_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014140.1"
                     /translation="MHATSLHSLAYRRFARNSSLFMLLLMR"
     gene            25995..28130
                     /gene="speC"
                     /locus_tag="ACHJ69_001102"
     CDS             25995..28130
                     /gene="speC"
                     /locus_tag="ACHJ69_001102"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO5014141.1"
                     /translation="MKSMNIAASSELVSRLSTHRCVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGANWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(28179..29435)
                     /gene="nupG"
                     /locus_tag="ACHJ69_001103"
     CDS             complement(28179..29435)
                     /gene="nupG"
                     /locus_tag="ACHJ69_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO5014142.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAEVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(29637..30716)
                     /gene="mltC"
                     /locus_tag="ACHJ69_001104"
     CDS             complement(29637..30716)
                     /gene="mltC"
                     /locus_tag="ACHJ69_001104"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO5014143.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQIES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(30781..31056)
                     /gene="yggX"
                     /locus_tag="ACHJ69_001105"
     CDS             complement(30781..31056)
                     /gene="yggX"
                     /locus_tag="ACHJ69_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO5014144.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(31084..32136)
                     /gene="mutY"
                     /locus_tag="ACHJ69_001106"
     CDS             complement(31084..32136)
                     /gene="mutY"
                     /locus_tag="ACHJ69_001106"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO5014145.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMVRFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVAALHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEDNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            32297..33016
                     /gene="trmB"
                     /locus_tag="ACHJ69_001107"
     CDS             32297..33016
                     /gene="trmB"
                     /locus_tag="ACHJ69_001107"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO5014146.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGIFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            33016..33342
                     /gene="yggL"
                     /locus_tag="ACHJ69_001108"
     CDS             33016..33342
                     /gene="yggL"
                     /locus_tag="ACHJ69_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO5014147.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLEEVRTSEL
                     FDVWWD"
     gene            33526..34245
                     /gene="yggN"
                     /locus_tag="ACHJ69_001109"
     CDS             33526..34245
                     /gene="yggN"
                     /locus_tag="ACHJ69_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO5014148.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            34421..35467
                     /gene="ansB"
                     /locus_tag="ACHJ69_001110"
     CDS             34421..35467
                     /gene="ansB"
                     /locus_tag="ACHJ69_001110"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO5014149.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            35584..36591
                     /gene="yggM"
                     /locus_tag="ACHJ69_001111"
     CDS             35584..36591
                     /gene="yggM"
                     /locus_tag="ACHJ69_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO5014150.1"
                     /translation="MKKQWIVGTALLMLMTGNVRADGEPPTENILKDQFKKQYHGILK
                     LDVITLKNLDAKGNQATWSVEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQNNSIPALKKDVKALDKQMVAAQKAADAYWGKDANGKQRTREDAFKKIHQQRDDFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVTILMKIDDINQVNERWKKDTEQLRRNGVI
                     K"
     gene            complement(36656..37792)
                     /gene="hemW"
                     /locus_tag="ACHJ69_001112"
     CDS             complement(36656..37792)
                     /gene="hemW"
                     /locus_tag="ACHJ69_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO5014151.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAANLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEPAPRVEFSQYTGLS
                     EEVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(37785..38378)
                     /gene="rdgB"
                     /locus_tag="ACHJ69_001113"
     CDS             complement(37785..38378)
                     /gene="rdgB"
                     /locus_tag="ACHJ69_001113"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO5014152.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(38386..38676)
                     /gene="yggU"
                     /locus_tag="ACHJ69_001114"
     CDS             complement(38386..38676)
                     /gene="yggU"
                     /locus_tag="ACHJ69_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO5014153.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(38673..39239)
                     /gene="yggT"
                     /locus_tag="ACHJ69_001115"
     CDS             complement(38673..39239)
                     /gene="yggT"
                     /locus_tag="ACHJ69_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO5014154.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFVKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(39257..39961)
                     /gene="yggS"
                     /locus_tag="ACHJ69_001116"
     CDS             complement(39257..39961)
                     /gene="yggS"
                     /locus_tag="ACHJ69_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO5014155.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGITGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            39979..40959
                     /gene="yggR"
                     /locus_tag="ACHJ69_001117"
     CDS             39979..40959
                     /gene="yggR"
                     /locus_tag="ACHJ69_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO5014156.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVKEGRL"
     gene            complement(41133..41549)
                     /gene="ruvX"
                     /locus_tag="ACHJ69_001118"
     CDS             complement(41133..41549)
                     /gene="ruvX"
                     /locus_tag="ACHJ69_001118"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO5014157.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRVHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(41549..42112)
                     /gene="yqgE"
                     /locus_tag="ACHJ69_001119"
     CDS             complement(41549..42112)
                     /gene="yqgE"
                     /locus_tag="ACHJ69_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO5014158.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(42221..43171)
                     /gene="gshB"
                     /locus_tag="ACHJ69_001120"
     CDS             complement(42221..43171)
                     /gene="gshB"
                     /locus_tag="ACHJ69_001120"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO5014159.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMADLY
                     LINGEARARTRTLSVEQNYDKWYEFTGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEDKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(43184..43915)
                     /gene="rsmE"
                     /locus_tag="ACHJ69_001121"
     CDS             complement(43184..43915)
                     /gene="rsmE"
                     /locus_tag="ACHJ69_001121"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO5014160.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(43995..44702)
                     /gene="endA"
                     /locus_tag="ACHJ69_001122"
     CDS             complement(43995..44702)
                     /gene="endA"
                     /locus_tag="ACHJ69_001122"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO5014161.1"
                     /translation="MYRYLSIAAVVLSTAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     VAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWDKMYPVTDRECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(44797..45294)
                     /gene="yggI"
                     /locus_tag="ACHJ69_001123"
     CDS             complement(44797..45294)
                     /gene="yggI"
                     /locus_tag="ACHJ69_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO5014162.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(45371..46765)
                     /gene="galP"
                     /locus_tag="ACHJ69_001124"
     CDS             complement(45371..46765)
                     /gene="galP"
                     /locus_tag="ACHJ69_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO5014163.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(47202..48356)
                     /gene="metK"
                     /locus_tag="ACHJ69_001125"
     CDS             complement(47202..48356)
                     /gene="metK"
                     /locus_tag="ACHJ69_001125"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO5014164.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            48412..48663
                     /gene="yqgD"
                     /locus_tag="ACHJ69_001126"
     CDS             48412..48663
                     /gene="yqgD"
                     /locus_tag="ACHJ69_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO5014165.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTQFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            48774..48917
                     /gene="yqgG"
                     /locus_tag="ACHJ69_001127"
     CDS             48774..48917
                     /gene="yqgG"
                     /locus_tag="ACHJ69_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO5014166.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            49011..49142
                     /gene="yqgB"
                     /locus_tag="ACHJ69_001128"
     CDS             49011..49142
                     /gene="yqgB"
                     /locus_tag="ACHJ69_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO5014167.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            49151..51127
                     /gene="speA"
                     /locus_tag="ACHJ69_001129"
     CDS             49151..51127
                     /gene="speA"
                     /locus_tag="ACHJ69_001129"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO5014168.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            51272..52003
                     /locus_tag="ACHJ69_001130"
     CDS             51272..52003
                     /locus_tag="ACHJ69_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014169.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIIISYSTNVPSGYDRVYSIRINELEYAIRDGNFKSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFVAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            52139..53059
                     /gene="speB"
                     /locus_tag="ACHJ69_001131"
     CDS             52139..53059
                     /gene="speB"
                     /locus_tag="ACHJ69_001131"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO5014170.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDIIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(53263..54021)
                     /gene="loiP"
                     /locus_tag="ACHJ69_001132"
                     /gene_synonym="yggG"
     CDS             complement(53263..54021)
                     /gene="loiP"
                     /locus_tag="ACHJ69_001132"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO5014171.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSSGLLSSGAEAFQAYSLSDAQ
                     VKKLSDQACQEMDSKATIAPANSEYARRLTTISRALGDNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVLGHEMGHVALGHVKKGMQVALGTNAIRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFTQRQESEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            54299..56290
                     /gene="tkt"
                     /locus_tag="ACHJ69_001133"
     CDS             54299..56290
                     /gene="tkt"
                     /locus_tag="ACHJ69_001133"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO5014172.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQIASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
     gene            complement(56509..57417)
                     /locus_tag="ACHJ69_001134"
     CDS             complement(56509..57417)
                     /locus_tag="ACHJ69_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFO5014173.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRENPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            57795..58238
                     /gene="cmtB"
                     /locus_tag="ACHJ69_001135"
     CDS             57795..58238
                     /gene="cmtB"
                     /locus_tag="ACHJ69_001135"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO5014174.1"
                     /translation="MRLIDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKGSTISNGPYYIIAPGVAMPHARPECGALKTGMSLTLLKQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            58266..>59500
                     /gene="cmtA"
                     /locus_tag="ACHJ69_001136"
     CDS             58266..>59500
                     /gene="cmtA"
                     /locus_tag="ACHJ69_001136"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO5014175.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYIDKSLEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPAPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETKSEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENV"
BASE COUNT        14349 a        14333 c        15573 g        15245 t
ORIGIN      
        1 ggtcatgaaa gttatcttta tggatttact gatttgttcg aagagtttct gtgcgtgttt
       61 tattttatca tgctcttcat gtctgagttc gcgtttgcca tcaacatttt tggtttcaat
      121 aataaggttc agataatcac cctttgacgt gtttacaaca taagcgaagt cagttatggg
      181 gcttgccggc gttctccggg gggaaagctc cgtgctactg tcacctggaa agccgttgcc
      241 gcaagcttgc tcctcgtaag tgttaacgga gcgccgtatt gacgcttatt cactggtgag
      301 tactacgttc catggggggg cgtatgcgcc gccgatgacg cgcccgaaaa acgcgtcaat
      361 atgagtccgc cggaacgata cagagtttct gcaagtaaaa cacggtgttc tccggagcac
      421 gctattttat atttgtttta ttgaatataa gagggggcca taaagcggcc cccttgtttt
      481 tatcagaaag agtaacggaa gctggcgttc accgcgttat ccacgttgta cttaccaaat
      541 gccgatttct caaactccag accaaagcgg agattatcgc gaatctcagc attcagacca
      601 acattcatga gcatgcgtcc gtctttctcg cctttgattc gtttctcacc agaggcgtcc
      661 cgcagaacgg tttcaccgtt attcagcagg tcaaactgat aaccgacacc ggcacgggca
      721 gtcactttcc agtccttacc ggagaacgat ttacccacat ccacaccggc acgaccaatc
      781 actggattga agtccttgtc tttcatggtc acgctcattc cgcgatcctg ccagtcgaac
      841 cgtttacctg atacggcccc ataaaccagt tccgcctgcg gctcaatcca ggcgtcttct
      901 gttacatgat agcggtagcc tgcttcagca cctgcataca aggaatgaga actgtagtct
      961 tttgttccga gtccggcaaa ggtcgccgag tactcattat catggtgaac atacttacca
     1021 atcaggtcga tataggcacc agagtcaaat atagcggaag catacagacc tgccccgacg
     1081 gacttcgtct tgccactgaa tgcattactg ctggcatgac tgtcggtata cgtcatagtc
     1141 agaccagtga aaaggtcaag tccatccagc tcatgttttt tatccacacc aatctgcaca
     1201 tgggtgtagt tgtcactgta tccaccagaa gctgaacctg ctccgctcat gatgcgggcc
     1261 catgcaccgg cctcgccatt ggtgtcacgc agatccccca ttcgtttatt caggttgttg
     1321 acttctgtca ggaaggcttt gtaattaccg gacatatacg attttgctgc accggaagat
     1381 gctgtatttt cagccacctt atatccggtc agggtccacg tggtatgttc ctgatcctct
     1441 ttcgtgctga tcacaggaga catgttgctg aaacctatgg cctgagtact ggctttaaat
     1501 atctctttat tgcttccttt tggggctgtt atcaggtcaa cgtttaatcc tgagttatta
     1561 tttcccactt tctgtatatc gaccgacaga ctgttattgc ttccggttaa ctgattttta
     1621 acaatgagtt tatccgattg atttacagac tggctcagat cagcccgcat aatcacagca
     1681 gtattagtgg catccagtgt gtttactgtc acagtatgga aacccgaatt ctctatgtta
     1741 ctaaacaaca aaagactgtc gcgggttttt aatgattgca gggctgactg tccgtttact
     1801 ctccataagg tgttatacat gctggctgag gcagcaggag catcaatacc tccggtataa
     1861 gaagtatcaa agccacccag caaacctgta gccagcggta tgtgtgtggt tttgtccagg
     1921 ttatcttctt tatcctggtc aacaccaaac agaatagatg cttttttgtc tgattttata
     1981 ttaccattga cccaggcata gttttgagcc tttaacgtgg caccatcttc tgtcatatta
     2041 tatccatctg acatatagat tgccgggctg tatttattat tttctgtagg ggtgcctgtt
     2101 aatgtcaggg ttccggccag ttcaatggct ttatctacat acatttttcc ctgggcggta
     2161 acagtagctc ctttgttgat aatcaggtct gtattaatta aggataatgc accagtcata
     2221 gtaacagttg atttatcacc aacagttatt ttgctgtttt gagcactcag actgccgttg
     2281 aaactacttt cgcctgctga atccccggag tgaacctgtt gtctgaatcc aaaaccttcg
     2341 ccagtaatgt ttcttcctga gaacatgtca atgtatgcag ttttatttcc cagaacaatt
     2401 tgagagtttt tagcctctat gtgtcctttt acattagcat cccggcctat actcagggtt
     2461 gcgttttcta aatttaattt ccggaaatta aatttacgac tttcccagtc gggctgggaa
     2521 aaagaggcta cctgattgtt ggacttatat tccgtattgt aacgtttatt tacagtgctt
     2581 tcctggtccc ttatggcagc agaatagtct ttttgacata aaagaggaat tagacagttg
     2641 ttactgcctt ctttaaatat ggcatgttct gtcgcatgtc cttgtaatgt taactgtgca
     2701 ttccggacag agatatcgtt tttgatatcg actgagccat caaagatcag attggtatta
     2761 ttgttatgtt tctgggtagt attgataata tgatttatgc ttatattacc tgatacattc
     2821 ccatggtaca tatatgcatc agtattagta agtgataatg ttgattgctt cacgtttgag
     2881 ttcgttacgg ttgttcccgc atctgttgct gcgattttct gaaatgactg gtcatagccg
     2941 tttagatcca gtgttccgcc atttctggta aaatagattt cgccattacc gctttcgctt
     3001 aaagcgtctt tggcatttat ttttaccgta cctttacctc cggccatgta aattttattg
     3061 aaggcttttt cagcactaag gatgacagtc ccattacctg ttttaaggtt atttccctgt
     3121 gctgttttta catccagagt accactacct attttatgca gattatctcc ggcaacgccc
     3181 ttaacattcc attcaacggt ggtattatta tcaatatcta tcccggcacc tttataagac
     3241 ttatcttttc cggacacttt atatgtctgg ttcttatcga atataagccc gccaatgcca
     3301 agatcggtat cctgcttgag cgttaattcc cctcctcctg agacagacag gtccttatct
     3361 tttataatcg atgataactc ttgtttttta ccatccagct gcatagtgtt tgtgaaatta
     3421 ttttttaaat tgtttacccg gtcattatta aataacgcgt agtttgtcca ggctatattg
     3481 gtactactga taaaaccaac tgtactggta acaccatgga aaacccattt ttgttgttct
     3541 ttatcatata gataagaacc agaaccacta tcgccaaaaa ttggtgagtt tttaaaaggg
     3601 gttctgttgg tataaatgta actcttgttt ttccagtcca gattaaaaag acttgcgctt
     3661 aacaagtcgg gattatagtt ttgtccaaac agatattgtt tgccgtttaa tatcgttgaa
     3721 gttgttccgg cacctgctct gaagccaatt atttgttcct taccgttgta gtttacgcca
     3781 tatcttttca tcgcatcttc gggagaaagt gagaagtcgg cactctccgt aacccctgtt
     3841 gtttccacga cgaatttatt cagacgatga accgaaaagt cgccagatga tactctgtta
     3901 cttgctttat atgctgtctg atcccatgac tgtgttgtta ttgcatgatg ctgtgtaccg
     3961 ttatgagtcg cagtaacact gtacgcacct ccaattgatg ttgttgcacc tttgttggaa
     4021 acagcagaaa agtcaggtac aggtagttct ggaaaatgaa atatctttcc atctttcatc
     4081 attaattgaa catttgttgc acccggtctg aactctcctc tgttttgagc aaggtcaaga
     4141 taatcgcggg tccatatatt atttatatcc agcattgctg caaatgctgt ttcgccataa
     4201 cttccatata ttgaggataa aataataccg aggataactt tctttcttga tgtaccgaca
     4261 ctaactttcc gggtcagttc agaaacagca actaatccac ctgtaatatg actatatttc
     4321 agtgaataaa ttttattcat acgtgaactc cgatatttct gagttgtggt tatgggataa
     4381 catttaatta caacgggtaa ctatcagtca cggcacgtaa ctgatagggg tataatgata
     4441 atcactgtca gttatatttg tatgacagat gtaacaaaat aaaaaaaaaa gataaacaca
     4501 agtgattgat tgatcagata ttgatcactt ggtgtttgtg ttttgaactc tgtgatcaaa
     4561 atttgatctt tgttgtttcc ggtcaatcct ttacaggttt tctgtattat ccgggggagt
     4621 taagtctgtc gatgtccgac agacttttga tgatggaaac aacatgcagc gataatgtgc
     4681 atgctgtttt gcaggtggaa ctcagaaggt gaactggaac tgtgcgaccg caccgtaagt
     4741 gttgtccgtg ccgatcaggc ctgcaacatc aaggtgaacc acatcaaacg gtgagatccc
     4801 caccccggcc gtgaatgcac tgccgtcgtt accggccaga ttctgacggt aaccggcgcg
     4861 cagctgtgcc catttccagg cattaaattc tgcgccaatg gcggcaaact gacggttgct
     4921 gtcggaggtg aaaccgctgg ccggggtcag atccacatca aatgcggtgg tgaacatcgc
     4981 gttgtgccag gagacaccgg ccgtcacctg cgggcggatc ctgaaggttt ccgtgatacc
     5041 gttcacttct tttgtctcaa tactacgggg gatcaggttc tgtgcgacca gccccagcgt
     5101 ccagttgtca tccaggtcag tactggcacc gatgtcggcg ttgatgccat ttttcgtgtt
     5161 gtggtagcgg tcgcctttga aggcgctgct gtcataattt ctgaccagca cgttgtagtt
     5221 gaacaggtcg acgcgctgga atttgggggt gaagcccaga gaccatttct gcccggccgt
     5281 ttccagttcc gtagccagag caatacccac atcagtgatc agtgccgcgc gggcaaaggc
     5341 ctgtgatgtc aggcggcttt tgtccacctc ctgtccggtg ctgtttgcca cctcttccag
     5401 gtaagtgagg tcagcatcag agactttacc gtcgacactg acggtgccgt gtgatttaac
     5461 catcagtgcg gcagagaggc ggtcaccggg aagggccgcc acagcagaaa cgcccaactg
     5521 tgcggcggca tgtgtgtgac ggaagtcccg gaggcgttct ttcaggcgtg cggcagcctc
     5581 aggaacaccg tggttactgt cgatggcgcg gtcgaaggcc ttccagtcat cactgatccg
     5641 gtcggcgttg tcggtgatat tatccgggtc ggagagttgc gcaccgacgg acggaagcag
     5701 aaggctgaaa tcatcgtcag gaccggcttt tgtcagcagc gccgggtttg ccagcggtgc
     5761 cacgccatag tgagaggagg cgacgccggt tccgcccatt gcatcattac gggcttcaga
     5821 atatgaggtg gcggccgctg aattaaagga aaataaaacg gcagacgcga caagtgtacg
     5881 ggtaatatgc tttttcatca tcatttcagt ttcctgtatt aaggtctttc gggtatagca
     5941 aaggcgtggc acgcctgtgc cattaaaaac agtcagtgtt taataacgtg ttattaccag
     6001 ggcgataagg gaattcggaa taactcagcc ggtaatattt ttcctgtgac cagatattcc
     6061 gcctgtgccg gactgagcgt gacggccctg acggggatat ccggtgacat ttttctggcc
     6121 atctgaaacc agccctcttt ctttttgttc attcctgtcg gatataaaga aagtaccggg
     6181 taaataatgg cattgttaat tttctttttc tggcgcagaa gaacgccaca atattcctga
     6241 gcgtgttcac cggcaaaagg aaaaccatgc cagagaataa tatcgatatt atcgtcttct
     6301 gaaataagac ggcagaacgc gtcctgtgtt tcgggccagt ctcccggatg acacagcgga
     6361 taatgcaccc tgccgttcag ccattcagga ctgagataaa accagtcagc cagtgaggca
     6421 aggatttccc cgcttaggta gtcagtgagt ctgtccggct cccggaagac ggcagggcgg
     6481 atattccagg gggccagtaa tgtggccatg gtgaccgggc tgagctcatg cacctgcatg
     6541 agatattcca tacggtcgtg aagacggcgg acgctgttat ccgcggggag aaacatctca
     6601 cttaatgcct cttcgaccag gatacgcgtc aggtcagaga cggagaggga aagctgttcg
     6661 ctcatgatgc tgagggcttc cctgacgccc tgtggataac gaacctgcag tggcgtgggc
     6721 tgcagctggc ggttgttgag cagggcttca caaatccggg ccctgtccag ggagagtatc
     6781 atgcgggcag caatgccagt gctgtttggt ttcattctgt tctccgtgcg gctcagcgcg
     6841 ttgtgttcat aagttgagtc taaagagagt gttttttaaa cggaaatgag ttatttcgat
     6901 cgttatgatc gagtgtttta aaagagtgtt aaccactctt tgtaggggcc cggggctggg
     6961 tgtggccaga tatgttgttg gtctgcattc tgcggcagaa gtggttattt gtattgcgtg
     7021 gggagtattg caggcgccct tgtttataag gtcataaggt tgtttccaga actgttgttt
     7081 attacatgaa gtcgtataat attaaaaaag tgaatccggc agtatataac actaacagaa
     7141 taatttgctt tggcagtgat agtttcttaa atctgtcact gtcatatgcc agccagaatg
     7201 cagtacagat ggccaggcct gataagaata tggatataag taatataatg gggagtggaa
     7261 tatgtccgga taccggaata ttatgccaca aatatatctg tatcaggacc tgagacagaa
     7321 tgactgtgat taagttaatc cggaatgtat agtttatttt cattctgata tgccttcagc
     7381 ttgttaacgg acaaatcatg acccgtgcat caatgtttgc gggtagctcg cctggcgttg
     7441 gaacgcgttc accccgctga gccgcctgtg ccagtgtgcg cagcaacaat acgctggcat
     7501 gagatattgc cagttcccgg gtttcagcac gggtttcaag gtcgaaatcg tgcagtgtca
     7561 caatgaacag tccgtttctg ttcccgactg ttgcgggata gggaacaaaa tagggggcga
     7621 ttttgcaggt ctgtttacgc tttgcatcga gcttgctgac cagctgatac gcccgcagat
     7681 gagtatatcg cttcagcatg ttgagagagt gatggccgga aatggccgca acctccatga
     7741 cgttgagtgt gccgagttca aaaaaacggc tgatggcttc atggcgaagg tcgtgaaaat
     7801 gcagattttc tattttcagg tccagaagtg cggtacgcca ggcgcttttg aaaccggatg
     7861 aggtgtaact gaacacattg ccgtttatct gttgtggcag aatttgcagg tagttgcgag
     7921 ctttccggga gagtgggaca tcgcgtggta aaccattttt cgtttcagga agatgcgcca
     7981 ctccgtgctg caaatcgagg tgttcccagc gtaatgtgag gatttccccc tgtcgcattg
     8041 ctgtttccag ggccagatgg aagataacgt agagctgctg atttttatct ctgaaatagc
     8101 gggagagtcg gcgttcttcg ccagaagtca ggcgacgatc gcgtccacta ctgattttgg
     8161 gttttcggac cagttcaaca ggattcatac ggcatgtccc ccattccaca ctggcgatat
     8221 tgaataatga tgataaaaga gcaagttcaa gacggacggt gtttccggtg atttgacgcc
     8281 ctgttcgtgg gttaatttga gccaaacgct gatcccggta agtggcgata tcaacggtgg
     8341 tgatttcatc catatagcgc tcggccatag ggtgacgttt aatcacattg acgcgataaa
     8401 actcctgcag atgacctttt ttgtggatgg atactgtctt caggtatttg tccagcgccc
     8461 ggttgagagt cattttgcga atttttattt ttcgaaacat atctgccttc cacgataaat
     8521 cccaggaagg taggaatact gaatcagaaa tgcaatgtct ctgtgccaaa tccggtatct
     8581 ggacaacgac aaaaatgaac ttcactacca gtacatacaa tatcgggaaa aatactcgta
     8641 atctgagcat tggcgtacat gcgtactgct cgtggacata ccttaatggc tcgccttttg
     8701 gtagttttca gcagatatac gccgacaaaa acaaggtttg gtatgtgaat aattatgcgt
     8761 ggggaaatta tgagtccggt ggcacaatta cagtgacatg cctcaacctt cctggtgccg
     8821 gaatctaacc aaaagggcaa cagtaagcat cgacgtgctc ttcatcaaca ccatctccga
     8881 tgccgtacat tcttgtacct gtcatgacgt acccggcagg gcactgaatc ggcttgtaat
     8941 aaatagaacc tccgcttctg ccaccgataa aatgtgcgga ggtcgcttca taccatttac
     9001 aggcagaata gttcacttta ttgcctccaa gccacaaacc ggatttggca ctgaagtatg
     9061 gccccgctgt tgtcgcggcc tacaagcccg tctggtgagc aatgagtgcc tgcgttggcg
     9121 attccgttta gctgtaaata ctcgccggca gtaagacgac catcagagcg gaccgttcct
     9181 ccagtgactg tctggccacg gacagttcca cctttgatct ctccttcggt tgtgatccct
     9241 ttgttgttaa cagcacgtat ccactggctg tccgtcatgg tgaagccgcc accatttgga
     9301 tgtaaccaac gatttgatgt gacatcgcct gaaaatctgg cgtttttacc actggcttca
     9361 ttgatattgg tgatgctgtt attattcata tcaatgccag tatgcattct gtttaaatcg
     9421 ggtcttcccg gaacgctgta gcggtaaagc cgatcgcttt cctccagatc ttttccagcc
     9481 attcgcatgg cgatatggcc cttgttgcag gtaatctgat aattacttcc cagatcggtc
     9541 cagccgccaa aggcacctgt aacggaatta ttctggagca taaaaccacc gagtccatta
     9601 atctggcccg ctatgcgacg taacccatct tctttaatat cctgtcctcc ggttgtgcag
     9661 gtaagtgctt ccagacgacc ggataaatta ttttttctga taccggtaat atagctgtgt
     9721 ccaaaggggg agcgggaaaa tccgttgttc agatatccct gctgaattaa ttcatttacc
     9781 gtgatggttt tactggaaag tgagctggtt ttatcagcta cataattttt tattgcatta
     9841 ttatattcat cagcatgtaa agccgcactt tgataaagtt gttcattcag ccagtcttca
     9901 atttctgaat aaatcattgg tgtggcgata ccaattacta tcaggccagc aagaatttca
     9961 agcagaataa aacctttatc gtcatcgagt ctgaattttt tcacgtgaat atctccgatg
    10021 tgtggataac ggaatcaggg aagagggtca gcacatacag ggacgcgcag agaaacggag
    10081 caaatggcag tgcctgctgg cgtctgcgac ggcagaaaat gaggataaac agaaatgctc
    10141 cggaggctgc ggcggtgatt atcagtggag tgtgttgcgg ttgtgtccac attgcgatcc
    10201 ccgcaatcag gaacacatca ccaaggccga gcgcttccgt ccctttcact gccagaaaaa
    10261 tgcgtcgtaa caggtatatc atgccgaacc acagtgacgc gttgagcagg gcatgagcca
    10321 gtgagtcctg gctgtatctg aacgctgcgc ccagaataat aagagaaagc gtgaatatcc
    10381 ggggaagccg acagacagca atatccgtca gcgtcattac agttaataat cccgtcagaa
    10441 tggaaaggaa taacagtcct gtcgcgtcaa caccatatgc accgttgagg caaatgctca
    10501 gtacagccag aagacaacag atgcacaccg cagcgttgtg tgggcgggta ttgtgtacct
    10561 gcgacaaata taccgtggcg gcgctgtatg caagatacag aattgccgca aggacaatca
    10621 ggataatgca tatcgggagt aattcagaca cagtcacctc agcttaatct tttctgaaat
    10681 accggttgtg gattattcgg gcatatatca tgcgccgggc tgtgttattt ttgctgaatc
    10741 ctgcgttgta actgccaagg cattcccagt taacgccgca gcgttgaaaa tggcgggcca
    10801 gtatccaggc gccggtatga atattcaggc agggattttt aagcagttca tccttgtctt
    10861 taatgacgcc cagttttttc agttcaggaa tatgcgatga attaatctgc attattccgt
    10921 aatccgtact gatgattttt ccggatttgt ttttattgtg atttatcgca gtggcgacca
    10981 ggccgctttc ctgctgtgca atggttttca gaagctcagg ggcaatcagg taacgttttc
    11041 cggcctgaat aaaacaggaa tcgagatgat tattattcat cacagatgcg attgatgtat
    11101 gactgaataa aatcaggaca gagaaatata accatttcat tatgataatc ccggcagtga
    11161 cagcatcctc ttgtatttaa ggatgctgtc cggaaacatt agctgtttgt ttcaagggtg
    11221 atgttatttt tgtccttact acatactgtt acaggatcca cagtagccgg ggtggtattc
    11281 atgattttgg taaacatgga tgaactgcgc aggctgttaa tgagttccac gcaattttct
    11341 ttcggtacat tattgctggt aatcgtataa ccatacttat ctgttgttgc cttaatggtg
    11401 acatcaccgc cccaggcatt tttggccttg ttaccaccgg cgatcatatc tgccggcagg
    11461 gaaccactct gcgtcagcgt tttgatataa tcactgctgg tatattgtcc ctggaacttg
    11521 tactgcttca ttttggtggc aatggtctgc aggtttttca gttcgcgctg gttatcactg
    11581 gatgacatgg tactggacat ctgacttatt gcgagaccaa ccacaatacc gataataatc
    11641 agtgcgccgg caatttccat taaggttaaa cctttatcct cctcttcttt ttttaaagaa
    11701 aaataataaa aaagacgtgc tctgaagttc atatatcact cctggtttat gggatgtttc
    11761 agaaatattc tgtatggtgg gggttattgt ttcagcatgt cgctgatact gtagatggca
    11821 gatatcatgt taagaataaa aaagaagaca aaacctcctg atataccggt aataattaat
    11881 ccgctgcgtt ttacccgttt ttttgcctct tccagccact ggtcggcata gaccgccatg
    11941 gctgccacat tatcctccct ttcggaattc aggagcagtt tattgatgca tactctggac
    12001 ggaaaatcgt aaccggaatt tcgcattgcc gtggccagat tctgaccgga taacacatgt
    12061 tttcgtattg cctgaattct ctcctttaac catttattct tttctgcccc tttttccatt
    12121 ctgagcagtg cctgcgaaag cggaatatta atctgcagca tggatgaaat actgaacaga
    12181 taattcactc cgtgtaaggt ctgatacagt gaccagggtg gtaccctgtc cagcatcctc
    12241 cggccggggc cggtgtacct gctgaaggaa aaacggatca gaaccgtcag ggcgataatg
    12301 gccccgcaga gccagagtcc gtacccgatg ataaattccg acatgccggc cagcactttc
    12361 atctggaatg accatttatc ttccgtggca aacgcactga cgatgggaat gatacgggta
    12421 tgggcgttgt acatgttcac cacacagagt gaaagcagca gaaggggata gaggcagacg
    12481 gcaatgaggc tggatgtcat ttcccgctgg gctttaacgg ttctggccac ctggaaaagg
    12541 gcgtcggcaa tattcccgcg ctcttccccg tttctgatta acgtggcttc ctccggtggc
    12601 acccaggtga acagggcacc ggataacatg ccgctggctt tgatggacgc gatgcattct
    12661 tttgccagaa aatacatgcc ggatgtgcct ttcttttcac caaaatcggt ttcgatctcg
    12721 cagagaaatt cgagggcacg cagggacggc gttccgtcac gtatcatgcc gcccagggct
    12781 tcgtagaact ccacccggtt cagggcaaag gtttttctgg ctatccagac ggtcagtgct
    12841 gtcagctcga tgtcggtact gtggagtttt tccagatatt tcatatcagt gcagcagcgt
    12901 atagctgtcc tcatcgacgg gagagatgta atgggccgcc agcggatcaa cctgtccgct
    12961 gttgattttt cccagcagat gctgattacg ggtcattccg cccagttccc ggtgccagta
    13021 ggttctggcc tcgatacgcc ccttttcccg gtacagctgg aaaaaacgcg catccgggga
    13081 gataacttct gcgatcaccg ttcgccccgt cacgccacgc cagcagtgcg ggcagccttc
    13141 atggtttctc agatatacct ggtccgggtt acagacgctt tccaccaggc gccgttcatc
    13201 atccgtgcgg tcggtggcca cctcatgcca ggggcgacgg cagtgcggac atatcagctg
    13261 aaccagacgc tggctcagca ggccaatgaa cagctgcgga tcggcaatca tccttgcctg
    13321 taccccctcc atctcaagac gttcgagaat gttaatcggg tcgttcgcat ggatggtggt
    13381 cagcatcaga tgcccggtca tggcggcttt tattgccgta atggcggaat cgtggtcacg
    13441 gatttcgccg ttcaggatcc cgtcagggtc aagacgcagt gctgatttaa tggagtccac
    13501 ccagccctgt gcggaatcca tgacggcagt ctgaatggca ccgggtatac ggccctctgg
    13561 cggggattca atggtgaaca gcctgcgacg gggcagaaga ataccgccgg tattgttgaa
    13621 gccgtactgt tcaaggtatg ccgcagaggc ggttctcagc gtggtggatt ttcctgaacc
    13681 tgttggtcct gagagcgtga taataccttc agggcgctgc agaatacggc gcagggtgag
    13741 ctcctggtca gggtgatagc ccagcatgct gaaggtcggg atttgcagac tgtcatcctt
    13801 gatcagacgg agcacagcat agaggccgcc cacgcaggga tagtgggagt agcgtgcacc
    13861 aaacagaccg gccagtttca ggtactgctc tgaaagacgc gcatcctgag gcaccccggg
    13921 atcaaactgt gtgcccttga tgacgtcggt catgccggaa taggtggccc ccagtaattc
    13981 gagaccttct tctttccgga ggatatcgag cacttccagt tcgccgtgaa cccgggcttc
    14041 cacataggtg aagtccgtgt tgtcgcgtcc tggtgtgata tgcagatctg aggcatccag
    14101 ttgagcggct tttctgatgt aatcaagaac ttttctttgt actgtgttga tccctccgtc
    14161 ggataaaacg cctggtgcac gttccgtatt attgctgttc agggttgcga taacgtcaag
    14221 agtggtaaaa tcgaatttgc tgatttttcc cggatagcgg gagcgtaaga gcgtcaggta
    14281 acgttgtaca gaggtgatgt gcttcttcgc ctcgacgact ttcaccctga tttttccgtc
    14341 agcgtcatgc tgaattgcga cgaaatcacg aatatcttca ggaggcagta cgatagtctc
    14401 tggcatgatg gcacctcacg ggcgtagcgt ctgcctgttg ccgttgtatt ccagaatcac
    14461 actgtccggt gtgatttgtt ccaccatgaa cagtgtaccg ggtaatgcct gtccggccct
    14521 gacatcagtt tcagttcctc cggggagtat gacgcgtgct gaaagctgtg gctgaatacc
    14581 aaagatttct ttcagacggg gggatgataa tgcctgtgaa ccggaggggg tgccagctgc
    14641 attttccggc tgatgtcccg tgacagaacc ggaaaccggg gagataccct gttccagtgt
    14701 ccgcaattca tgacgaattt tttctcttgc cagcatcgct tcatacaggg cggtttcttc
    14761 ctgaatctgc tcaatctgca gttgtttgtt ctgtgacgcg tcattttcgt tgccggccac
    14821 tgccattgcc ggtaacagca gggctgcaat cagcagcgct tttgttttat cgtgcataat
    14881 atttacctgt gaactgataa cggaaacgac cattttccag ttgtctagtg acggagagaa
    14941 aacggacaga tggctcgctg aagcgctctg ccagttggtt agccgggagt agcgtctgta
    15001 tctgcaggtc atactctttc cacggtggct ggatgatgtt tccgttttca tcagtgaagc
    15061 tgttattgac ttccggccag tcaatctgca gttgaactga ctgtgcgagc gtggtcagtc
    15121 gttcctgcag gtctcgggtt tccggaaggg aatcaaccgg gacaggatta tccgggagcc
    15181 ggaatgtgac cggcagtgca acgccgccag agttcccttc aggcataaca ataaacggcc
    15241 gggtgtctgt gagttcagtg atgcgccgga tgaaggctgt cactgtcgtt cctgctgtat
    15301 tggtgtatga agaccgcaga ttaccgctgt taccgtcaga agaacattca gccagggtat
    15361 aacgccatcc ggcagcagac agttgcgtct ggttccatgt tgtgctgcat tgccggataa
    15421 attctccggt ctcaggctgc cccgtccatg gtggcgttat acgtgcttca gctgatttcc
    15481 gggcgctttc ttcccgttcc gctttttccg cctgccagat ttcataacgt tgccagccgt
    15541 accacgatgc gccaagcagc gtgatgatga gtaaggctgt cattaactgt gggcctctgg
    15601 acacggggag cagacgcgct tctgccggat gctttctggt gctgatcagg tttgacagcg
    15661 gtagcgggat acaccgactg tctggtgaaa aaccgtcagg ttcgtagcgt acccagccgt
    15721 tttccggttc cgcattgagc atcaggaacg tgttgcgggc ttccgtgatt tcagtcgggg
    15781 taccggtaac gtcgttaacc agaatgccat taatcaccga aacaaacgca caggtgagtt
    15841 cagcgtcgtc gttctcgtcg ttcccgatga tgaaaatggc atacccacct gatttgagta
    15901 gcgggcgtat taacattgcc agtgacaggc actggcgaat gttacggggg atatctgcat
    15961 cctgacgact gagcagggca atcatcattt catcaccgtt ttcaccgtcg tggttgtggc
    16021 aggaaagata cagttctgcg ccctgtgacc gggcaaaccg ccgggcattc cggtaaccgg
    16081 gaggtatgtt cttccagtca aggcccgcaa ggaatgcctg tttacccgat atcagtgaaa
    16141 ccggacgaat ttttttctgc attgacccgt tcaagggaac ctccgtcaga ttacgtgcgg
    16201 tgtgatcagc acaaccagaa tggtgtcgtc atccgcactg tccacgccac caccgagtcc
    16261 gaagaatgac ggggtgccca cgccctgtct gagcgatttc cggctgaact gctggaatcc
    16321 gctcaggatc accgtctgat ctgatttcag gtcgatggtc tggttgatgg ttttcagctc
    16381 aacactcggt gtctgggccc gactgccacc gctttcaaac acttcaaaaa caggtttgtc
    16441 ggacaggctc atgctgaaca gtaactgcaa ccggtgtgag ccgggctgga taaacggtaa
    16501 cagtgtcatg ttgaagccgg tggtgattgt tgccgcattc agtgaggtgg aggagcccac
    16561 attggccgtc gtatccgtgg tgacactttc tatgtacccc tgctggctgg cgacctgcat
    16621 aggaaccggc gtcaggtttt tggtgacagc cgagttttgg gtgaccacac tgactttacc
    16681 ctgtgatgaa agcgctttca gaaaggcttt tgaaccggtg agcttaccgt caataatcga
    16741 taccccactg gtcacgactt catcggcggc attagtaaat gcactggtca ggtcgatacc
    16801 gagatgtccg tcactgaaaa cggcgttcca gtcgattccg gcctgctctt tgctggtttt
    16861 tttgatgctc agaatttcaa tattcagcac aacctgacgg cgcatttccc ggttacgttc
    16921 ttccataaaa ttcttaacag tatccagaac ctgtggggtg tcggttacgg tcagaaagcc
    16981 ggtggacaac accatccttc cgataccggg cgtcagcatg gaattcacgg cattttttgt
    17041 gtcttcatag agtgtggatt tcatatcaac ggcggttgtc tgcgtattgc tggcatcccc
    17101 actcagggaa ctgccggatg aaccaccgga actgcccgtg gatgacatgg tgccactgac
    17161 gactctggca ttgtaggcga cctggctgtc catgtaggtg accgggaatg tacgggtgtc
    17221 aagccagtgg aattcaattt ttcccttcac atagcgccag gtgatcccaa agcgtgaaga
    17281 tacggtgtca agcaaagcgg gcagcccctg aacattgagt tccggcagtg aggccgtgga
    17341 gggggcggct gatgacagcc ccacggtgct cagtggctgc atgccactgg tatcaggcgg
    17401 gggaatgtcg ccgctgatcc tctgtgtgcg attttctccg gtacccagca gtgtccagac
    17461 atcagacatg atttgaaccg gaatatgaca ggtttcagaa atccgctgtg caatctggtg
    17521 aagtgtgatg gcttcccggg tgtttatgac gaccgggcaa tccggtattg cccgggtaac
    17581 ggttttttcc ggaaggggcg tggtattgat ccactgctgg tttgtttcgc gcacaacggc
    17641 gcgttgtttg cgaagttcac cggctatacg ttcagcccgt gttatctcat cggatgcttt
    17701 tttttctgtg gcactgattc gttcaaaact gctgcagcct gataaaacaa cggataaggc
    17761 ggtgacagcg cagaaaaatt ttttcatatt ctgtatacct gcttgttgaa tatttattct
    17821 ctgtctgtgg agggacgaaa taacaattca gtgagacaga gcgacgggta tttcatgtgt
    17881 taatgaacac tgagaaagat tttccggact gaagtctgcg ccgtagcgat atatcatgtt
    17941 gctgagagaa gacatgatat gcagacctca gtgcagaatc cccttttggg gatgcttaat
    18001 tgatcaggac gatcgcattt tccggtataa cggaaggcgg aacaccaata ttatttccct
    18061 ggttatccag taactttctg tccctgacct gcgcaattaa tgctgagttt cggctcactt
    18121 cattcggggc tgatatcagc cctggctgaa aaggaagcca gacaaaagtt ctttgccctg
    18181 aaataatatg ttttgttcca tataccggag cgaatccggt ttcgttaacc gtaagcatct
    18241 ttccttcacg gatatcaccg ggatgatgat atttccagtc attaatggca tttgtcaggc
    18301 gtaatatttc atatacctgt tgttgtcttt gctgctcatc agtatttttt attgcccgct
    18361 ccgtattgcc ggcatgaaac cagttcccga tgataaacag tgccattatg aggggcatga
    18421 gccatcccat ttagctgtcc cccgggatgt cagtaattat cagcacgcac tggtttttat
    18481 tcaccgaggc atataagggt ttttcagcct gctgataaag tgcgaatgta ctgttcagtg
    18541 catccataaa attacctgaa aaaactaacc gggcatcgat actgtaattt accggcgttt
    18601 tccatgccac tttccatttc ccggtagcgc agggttcggc ggctgcccat ttttccagcc
    18661 ctgtacgcag ggtcattccc tgctcaatat gacgtgtttc tgtttttttg acaccggtta
    18721 cgggggcaga aaacaggcca gattttaccg ctttttccgc ctgctggatc agtacgcgtt
    18781 tccctgaggt atcaaatgtg gctttcaggc tgtagcgggg cagcgttttt tcgataacat
    18841 ggttccactg gtcatttccg gaccatgata ttctggcggg gagccggacg tcacgggcaa
    18901 tctcagtacg ccagcctgtg ggtaataatt gtttcatagc ctgaccaaac ggtcgttgtc
    18961 cgtcggcccg tataaatttc agcgtaccga ttgcacgttt ttcggcgtgg ctgtgcgatg
    19021 atgatatctg agtggaggct gtggcggtat atgaaaatga gaacgcgaag agcgaaatac
    19081 ttaagtattt taatatattc atcagtatca cctttttcac tatttctgtc tgttaagacg
    19141 gagttaactg aattaataaa aagtggcgta gataaaatgg gtgcatgaat tgattaaaat
    19201 aagatgaaga aaagccaggg ttatataaat aaaggtgttt ttgtttctgt gtatgtcgca
    19261 gagatcacaa atccatgcgt gagttaataa cgaattacag aacgccagtg aaatcagcga
    19321 gtttgtaatc gcattgtccg gtgtactacc gaaaaccggc gtgctgtctg aaatactggt
    19381 gcctgtcacc aggaatgctc agaatattga tgaaaggaaa tatgccgaaa aaagagacag
    19441 aaaatccttt ctgtatctat tgtaaaagcc tcttacagtc atatctgcgg ggatggtcgt
    19501 ggtgatggct attataaaag tgataataaa aataacgata acgaaaaaca gaaccaccgg
    19561 aagaaaattt gtatatgtga tgggatgagg cataataaaa atcactctcc aggtgcaggt
    19621 ggattttcct tacatgaaaa ttgagtaacc ccgactttaa cggtgaatga tttaccggct
    19681 tctttttcag tggagaagag tttttcacac tcctgtttcg tcatgggcgt attctggaag
    19741 cgaatccatg aaaccggtgt accgccgggt ttttcgatgg tggcctgaat acagtattta
    19801 tacgggaccg gggtggcctg tgctgatggc attatctgta ctcctgctgt gcattatcat
    19861 tctgtgctga aaaaaggcga catcaccatg gggaaaatga tatcgccgaa agtgcctggt
    19921 tttactggct gttgttcagg actctgcagg gtgcaggctc cggcacaacc ggaaacgtac
    19981 tgttctggcg ctcagtgcgt tttcgggttg tgtagccatt atttgcggtg tccggtgttg
    20041 ggggagtgtt acctgtggct gcaggcgcgg cacgtcactc tctacgtagc atatctgttt
    20101 tcctcctcag cccggcttca gggcgtggcc gctcccggat tgttaaagag catgaccgtt
    20161 acgaagaacg gtgatacagc aggcccgttg tgggctgtgt ctgaggggat aatgaacagc
    20221 ggatacccgg gtggcgataa ttaattcttt tcaggtcgct aaaaatttac gggcggtaaa
    20281 attttctggt caccagggta aattaatgct ggtcacggtc gcctggctga aggcagagtg
    20341 accggaaatt tcgtctttac ggaggaaagc catgccccat aaaccgacag aagaacagac
    20401 ggcaattatt cactggcagg ggaggaagct tgttgttaat gcttttgccg gtacagggaa
    20461 aactagcaca ctggtcagct atgccatggc ctgcccggag agcaggatgc tctatctcgc
    20521 ctataatcgt gcgattcgtg atgaagctga gcgcaggttt ccttttaacg ttgaatgcaa
    20581 aacgtcgcat cagctggcgt ttgcccgctt cggaaggcat taccagaagc ggcttgttcc
    20641 gggcttgttc gtcaccgatg tggcaagaaa actgaatacc cgatactggg cactggcccg
    20701 ggtggccatg acagggctga atcagtttat ctgctcagcg gatgcaaccc ccggcaggca
    20761 gcatctgccg gacaaggacg aaatgaaagg cgttgctccg gatgaggcac ttcgtgctgt
    20821 gaggacgctc tggcaggcca tgagcgatcc ggatggaacg tttccggtca cccatgacac
    20881 ctatctcaaa ttgtttcagc tctccggacc tgatctctcg cgcagatggg acaccattct
    20941 gtttgatgaa gcgcaggatg cgaatccggt gacttccgca cttgtgctgg gacagcggtg
    21001 caggattgtg ctggtcggtg accggtatca gcagatctac cgttttcgtg gcgcggatga
    21061 tgccctgtct catccgtctc tggccggtgc cgaccgtctc tggctgacac agagttttcg
    21121 ttttggcccc gcagtggccg cgatggctaa tctgctgctg aaacgcgcag gggaaagtcg
    21181 ggaggtgagc ggctgcggtg gtgatgatga ggtgcttacc atgcaggaaa tgcgtgagcg
    21241 catgaagcgt ggaaaatttg ggggacaccg ggcggtactg aaccggaccg tcgccggagt
    21301 gattggcacg gcgctgatgg cggcaatggc gggcaggaag gtttactggg tcgggggcat
    21361 tgagggatac cgaacggagg cgcttgaaga tctttactgg ttccagatgg atatgccggc
    21421 ccggatgcac tctgaccggt tgcgcaggga ttaccggaat tttgatgaat accgtcatat
    21481 tgcgaaggtg acgaaggatg tggaaatgag tcagtccata cgactactgg agctgtgttt
    21541 tccgttaccc aaaaaactgg agctactcag ggagcacaca gtcacgaacg agcgggaggc
    21601 tgatatcacg gtttcaacgg cgcaccgcag taaggggctg gaatgggagc gggttgtact
    21661 gggggatgat tttcaggata tcgctgatcc gttgatgtca gaacaggagc gccgggatga
    21721 aactaacctg ttgtatgtgg cggtgacccg ggcacgcagg acgctggtac tcaatgaact
    21781 catgcagcaa ctggcggagg aaaagagcag gacgaaggag aagacgacgt ccccgcatca
    21841 gcgtggcaca gagaaggcgg tggagggaaa ggatgtttaa gttacggaag ttttttacct
    21901 ccggttcagc agtggagata cccgatgacg ggcggtcagt gccgggaaca ccggaaggct
    21961 tttatgcgcc gctgacgaca gattcactgc tgatgccgaa gaacagggcg cagtgtctga
    22021 agcagatacg ggataacaat atgatgccaa agccattgtg tgaggcgatg tgttgtgctc
    22081 ccgtatacag cctgcttctc tccacgcaga atgtcccggc ggcccgggag gggcgctggt
    22141 cacatgcagg gggattcggg gacctgacgt tgttgcatac cagttacgct gtccgtctgg
    22201 cccgtgcaca tatgtttccg ccgggcgcct ctgcggaaga gcaggccgca cagggggcgg
    22261 cctggcatgc agtgattttc tggtcagccc tgttttatca cctgcctctg cttgcgcatc
    22321 tggaaggcaa attgttcagc ggccggggat ggcagccggg aatatccgtt cctgatgagg
    22381 cgtatcgttt ccggttcagg cagctcccgc cacagggtac ggaagctcag caactggcgg
    22441 cagtgattgc cggtggtctg ttacctgagg gggctgcagc ctggcttgcg acggtgccgg
    22501 gagcgcttca gaatctggcc ggtgcgttct ggcatcagca tccggaaatg gcactcatcc
    22561 gtgatgttct gcaggaggcg gcccgccagg ctgaaagtcc gttgttaaac ggtggaattg
    22621 ttgccacagc cagtcatatt gcttcagcag aggtctttac gggggaggtg gttgtatccg
    22681 gtaaaacgga gagcaccgtg gcatcggctg tgtccgggga agaggctgcg gctgaaggga
    22741 ataccgtgtc cggcaatgct gcagagtctg ttcaggaaca accagagatg cggcaacaac
    22801 aggctgagaa accgacgaca ccagacagca gtgaaggttc agaggacgat gcaggatgtg
    22861 aaacggagag tataccgaca gatgaagata cagctatgct ggtaagcatg tttgcggagg
    22921 aggcgagggc gccggaaaag ggggaggatg actcgccatc tggcgaggtg gttgcgcctg
    22981 tttcatcgac aggagagata tcagcagcgg gccatgggga aatctgtcag tcgtatcaag
    23041 acgctgagca acaggaatac tgtgaagcag aatctgatgc tgttaatcct gatttaacag
    23101 gagagcggtt tcttgaatgg ctgaaagacg gattgaagaa tggttcactg tcgtttaaca
    23161 ataaaggcga taaaattcat gtggttgccg gctttgtctt cattccggtg ccagggattt
    23221 tttatgatta cctgaaagcc cggcaactcc ctggggagaa gagagaggtg ctacagactg
    23281 catttgaacg actgaatatc cataagcgaa aagataatca gcgtttttat catgcgcgaa
    23341 ttgctgaggc accagggaaa acacacggtt ataaaatact caaaggttat ctgataaaag
    23401 gacgtggtct gatggataca ggggatctca atgacagtcc gaactttttt ttcagtgagc
    23461 gcaggaggtc agtattttgg taaaaatgta acctatttgt aaagggttta attatgaaat
    23521 taagtcatgc tgacatgcgc tggaacgagc ttatggatga atattttttc tgccgttcag
    23581 tgagagtggc tacggagtgg agttaccgaa aggttctgaa tggattccgg aagtttgttg
    23641 gcgagaccct tttaccggag gatatccggc agcagcatgt gagggaatgg agacgagagg
    23701 ttctgaaaaa acagaatcgc tccacgcaca catggaataa caaggttcgc catatgcgtg
    23761 ccattttcaa ttttgcatgt tcctctacac ttcttaattt aagtgagaat ccgtttgatg
    23821 gtatgaatgt cagacaggaa aagaaacgta agaaaacact gacgaagaaa caggtaaaac
    23881 gaattctgct gattcttgaa cagtttcatg aagaggagaa gcgttctgat ggtgtgcact
    23941 ggcgtagttg tgcgattcgt ccggcatggt actggatgat tgtggtaaat acgctgcgtt
    24001 ttaccgggat gaggctaaat cagttacttc acctgcgtct gcgggatgtt aatctggatg
    24061 aaagatggat tgatctgtgt tcggagggaa gtaagacaca ccatgagtgg cgtatcccta
    24121 tggtcagtca tcttgtcccg ggaatgaggc acttgattga tgaggcgatg aaacgggggg
    24181 caggacctga ggattatctt ttccattatc atcgttatgt ggcattgatg tcagaagctc
    24241 cgttcgttac cggacttccg gaactgcaac cggtaaaagg atttttcaga cgtctaacga
    24301 aagagtgcgg tttccctgta tcacctcatc gctttcggca tacgctggcc agtacgctga
    24361 tgagtgcgcc ggaacgtaat ctttatatgg tgaaaaccat gctcgggcac aggaatgttt
    24421 ccaccactct ggagtatgtg gaaatcgatt tgaaggggac ggcaagagtg cttgaaaaag
    24481 aactggcaga acttactgat gtattttaga tcatgacttt gttttgccgt tgttgacaac
    24541 ctgaaaaagc tttgcaagca taacgaatga tcgagagaag gatcatgcga ccaacatgct
    24601 ccttaaatcg agaaaatgag atggcatgag cgcattcacg acatcttaca ccttcccgat
    24661 gacacccgtc agggttctca ctcctgacca gtggctttga tgtaaggtgg tgcccggact
    24721 cggaatcgaa ccaaggacac ggggattttc aatcccctgc tctaccgact gagctatccg
    24781 ggcaacgggg cgcattaaac ctgattcgcc tcgtctcgtc aatcaaattt cttcaattta
    24841 gtgcagactg cacaatctat catcactttg ccgctattgc acacattgtc aggcaaacca
    24901 ggcggtccag gcggcggaaa gcggcattgc cagaaggagt gctggaagca tgttgaccac
    24961 cgggaacatt ttaataccac agatgcgtaa tccggttgcc agcagcaata aaccgcctac
    25021 ggcgctgaag tctgccatca tcgacggtgt ggtcagcggt aatatcagcg cggcagccca
    25081 cgccagtgtt aactggatga tcagtaatgg aatgctaatc accgacaccg caatacccag
    25141 cgagcaggcg aagatcatcg ccgtaaagaa atcaagaaat gacttggcga ttaaaatact
    25201 cggatcgccg gtcatccctt cgttcatcgc cccgaagatt ccggtgccgc tggcgcaaaa
    25261 cagaacaata atcgcgacaa aattctgaat aaaagattca tgcgctggct tcttacgcga
    25321 gtggtgaaac agattatgcg ctttggcgac cactgtattg acgccttttt ccagcagaca
    25381 aatttcgccg attaatgctc cgagtaaggt tgctaaaacc atcgccggaa ggttggcgca
    25441 tttcaccacc agtaaaatac caatccccag cgatgccaga ccaaaaattg atgtcatgga
    25501 gacgcggata cgttccggta agcgttggct aaggagtgcg ccgaaaacgc cacccagtaa
    25561 gactgcactt gcgttgataa aagggccgat gaccacaaga gttcctgtta gctgtattgt
    25621 ttgattttct tattatgaac ttttggcgcc gtcgatggct tgtttgttcg caaagtcctg
    25681 gcttgcacgc tttagcgaaa ggtgccatga ttgcgcgaat tttctcctct ctgtacggag
    25741 tttgcccgat gcacgccacc tccttacatt ctctcgctta tcgccgtttc gcgcgaaact
    25801 cttctctttt tatgctactg ctcatgcggt gaagttaacg cacgctcact gcaggacaac
    25861 agtaaaatca gagcgtttct gcttttactg atgtctggcg gtcggagctg gtgaccagtt
    25921 tgacccatat ctcatggggc agggttttcc accttgtccg gtattcttac ttcccctaaa
    25981 cgggttttgc gcttatgaaa tcaatgaata ttgccgccag tagtgaactg gtatcccgac
    26041 tttctactca tcgctgcgtg gtagcgttgg gagatactga ttttacggac gtcgcggcag
    26101 tcgtcattac cgctgcggat agtcgcagtg gcattcttgc attgcttaag cgcaccggtt
    26161 ttcatctacc ggtgtttttg tattccgaac atgctgttga attacctgcg ggcgttacgg
    26221 cggtaatcaa cggcaacgag cagcagtggt tggagctgga atccgcagcc tgtcagtatg
    26281 aagagaattt gctgccaccg ttttatgaca cgctgacgca gtacgttgag atgggtaata
    26341 gcacctttgc ttgccctgga catcaacatg gtgcgttctt taaaaagcat cctgccggac
    26401 gccattttta cgatttcttt ggcgagaaca tctttcgcgc cgatatgtgt aacgctgacg
    26461 taaaattggg cgatctgctt attcatgaag gatcggcgaa agatgcgcag aaatttgcag
    26521 ccaaagtctt tcatgccgat aaaacctatt ttgtgctgaa cggcacatcg gcagcgaata
    26581 aagtggtgac gaatgcgctg ttaacgcgtg gcgatctggt gctcttcgac cgtaacaacc
    26641 ataagtcgaa tcatcatggc gcgttgattc aggcgggggc gacaccggtc tatctggaag
    26701 cttcacgtaa cccgtttggt ttcattggcg gtattgatgc gcactgtttt aatgaagagt
    26761 atctgcgcca gcaaattcgc gacgttgcgc cagaaaaagc cgacctgccg cgcccgtttc
    26821 gcctggcgat tattcagctg ggaacctatg acggcactgt ttataacgcc cgtcaggtga
    26881 tcgataccgt tgggcatctg tgtgattaca ttctgtttga ttccgcgtgg gtcggttacg
    26941 aacagtttat cccgatgatg gcggatagct cgccgctgct gttagaactt aacgaaaacg
    27001 atccggggat ctttgtgact cagtcggtgc acaaacagca ggcgggattc tcacagacgt
    27061 cgcagatcca taaaaaagat aaccatatcc gtggacaggc gcgtttttgc ccgcataagc
    27121 ggttgaataa tgcctttatg ctccatgctt ctaccagccc gttctatccg ctgttcgccg
    27181 ccctggacgt taacgccaaa attcatgaag gggagagtgg gcgtcggctg tgggctgagt
    27241 gcgttgagtt gggtattgag tcgcgcaagg ctattcttgc gcgctgtaag ctgttccgcc
    27301 cgtttatccc gcccgttgtt gatggcaaat tgtggcagga ttatccgacg tcagtgttag
    27361 ccagcgaccg ccgttttttc agttttgagc cgggggcgaa ttggcacggc tttgaaggat
    27421 atgccgcgga tcagtatttt gttgatccgt gcaagctgtt actcaccacg ccgggtatcg
    27481 atgccgaaac cggcgaatat agcgactttg gcgttccggc gacgattctg gcgcactacc
    27541 tgcgtgagaa cggcattgtg ccggagaagt gcgatctcaa ctccattctg ttcttattaa
    27601 ctccggcgga aagccacgag aagctggcac aactggtggc gatgctggcg cagtttgaac
    27661 agcatattga ggatgattcg ccgctggctg aggtgttgcc gagcgtttat aacaaatatc
    27721 cggtgcgcta tcgcgactac accctgcgcc agttgtgtca ggagatgcac gatttgtatg
    27781 tcagtttcga cgtcaaagac ctacaaaaag cgatgttccg ccagcagagt ttcccgtcag
    27841 tggtgatgaa tccccaggat gcgcatagcg cttatattcg cggtgaagtg gagttggtgc
    27901 ggattcgtga tgccgaaggg cgaattgcgg cagaaggggc gttgccttat ccacctggcg
    27961 tgctttgcgt ggtacccggg gaagtctggg gtggggcggt tcaacgttat ttccttgcgc
    28021 tggaagaagg cgtgaatttg ttgccgggat tttcgccgga gttgcaaggt gtctatagcg
    28081 aaaccgatgc ggatggcatg aaacggttgt acggttatgt gttgaagtaa gaataaaaaa
    28141 acgggtcacc ttctggcgac ccgtttttct ttgcgtaatt agtggctaac cgtctgtgta
    28201 cctgtcggga cacgaacgtg tttatatttg aacatcgcca tgaacgcgaa ggccagaacc
    28261 acggagtaac ctgcgaaaat cagccatacg gtctgccagt cggtaatgcc gttttgggtg
    28321 tacatctcaa ccactttacc gctcacgatg ccgccgagga tacagccgaa gccgttggtc
    28381 atcatcagga acatcccctg cgcgctggcg cgaattgccg ggctaacttc tttttcgaca
    28441 aacaccgaac cagagatgtt gaagaagtcg aatgcgcaac cgtaaacgat catcgacagt
    28501 accagcagta cagtaccgaa cggagtcggg tcgccgtaag caaacagagc aaaacgcagg
    28561 atccacgcca caatactgat catcattacg ttcttaatac cgtagcggct taagaagaac
    28621 gggatggtca gaatgaacag ggtttcagag atctgcgaaa tcgacatgat gattgacgca
    28681 tgctgcacga tgaagctgct ggcaaacatc ggatctttgt caaagctgtg caggaaggta
    28741 ttaccgaaca tgttggtaat ctgcagttcc gcgcccagca gcattgagaa gatgaagaag
    28801 attgccatac gcttgttttt aaacagcgcg aatgcatcga ggcccagcag ggttgtccag
    28861 ctctgattcg cttgctgttt agcaaccgga atatgcggca gagtcagggt aaacagaacc
    28921 agaatggcgg aaagcgctgc gccaatatac agctgcatgt ggcttaattc gaagccggac
    28981 aggctcacca cccacattgc catgataaag ccgatggtgc cccagatacg gattggcggg
    29041 aagtcagtaa cgatatccat cccggcattt tgcaggcgat agtatgagat ggtgttgatt
    29101 aacccaagcg ttggcatata agcaaacgag ttaatcaata tcacaaggaa catcgcctcc
    29161 ggcgtagtga cctctgccgc catgaacagc gtgatagcgc caatggtgtg gcaaatggca
    29221 tagacccatt tcgcacttaa ccatttgtcg gccacaatcc ccagcagcgc aggcataaag
    29281 accgcggcga tacccagtga gctataaact gcgccgatag aagcaccgtc aaacttcagg
    29341 gtaacaaaca tataggagcc gagggtcgtc agccaacttc cccacagaca gaactgcaga
    29401 aaagagagga ttttcagctg cagcttaaga ttcatgttaa tttcctcaca tcgtgatgcg
    29461 gatgagtgtt tgaaaaagca catttgaccg tgcgagatga tgcgattcta tcaacttcgc
    29521 ggactggttt tttgttacct gtggcaaata attgcaaaca ttcccaattt gcaaaacgaa
    29581 attgtgatgt ggataacatt tttacccctg agcatcgtca ggggcggtta gtggaattat
    29641 cggcggcggt aagatttttg cgcggtattc actttataaa gataacggcg agattccgca
    29701 gagggatggc gggtcgtcag cgtctgataa acatcgcccg gcgtcatggt gttaataata
    29761 ttggcagcct gaatcttatc attcgaaaag actcgcagca cgctgcctgc gccgccgtta
    29821 taggcggtga tgacggcata acgccgcgat gttgggttat caattccgcc gagataaacg
    29881 ttgttcagca tcgccagata cgcggtgccg gtatcaatat tgctggcagg atcaaacaag
    29941 aaactgcggc tcggcgtgcc ggatttcccc tgcgagcgga acacatcttt cccggcggta
    30001 tgttgtacca cctgcattaa tcccagcgca tcggaacggc tgaccgcata cgggttaaag
    30061 gaagattcaa tctgcataat tgccagaatc agcgactcat caacgccata tttgcgtgac
    30121 gcctggcgga ccataccgag atatttgtgc gcacgtttat caagatggtt cggcaccatg
    30181 ttaatggtga cgctgtagat gatacgcagc ccgttgctgc ggctcttcag acggtttttc
    30241 agcagataat ccgcgaagtt gctcgcgcga ccttcccagc gaatcggctg cccggtgttg
    30301 tccaccacct gaccgtaaag gaaaggttct ttcgaaatcg taatatcatc aacgtcggaa
    30361 tagagatcga ccgaactcgg atcgtccccc atcagcaacg ttttgattat tgcccggcgc
    30421 aaatgcgcgg caggttctgt cccggcgatg gtttcgatag taatcgtacc gtcatcgaag
    30481 ttgatgtggc tgcgggtctg atattgatcg gtgtatttca cgtagtcctt aggaccggcg
    30541 atcaccacct ctttgaagcc ccagatgttc tcaatattgt gggcaaattg ccccatcaga
    30601 atatcaaaac cgttggtatc tttgacccag gcttcgttat aggtatcgcc ttttttggtc
    30661 gtcgaacagg agatgagcaa cggcgcaatc aaagccagcg cgagatattt tttcatcatt
    30721 ccgggtgcgt gttgtgtttg tttttatgca agttgccgga ggcgtgctcc ggcactgttt
    30781 ttatttttta tcttccggcg tatagccctc gatatgcacc tctttaccct cgaacaggaa
    30841 gttgaccatc tcctgctcca gcagcttgcg gtgctcggca ttcatcatgt tgagtttctt
    30901 ttcattaatc agcatggttt gtttgtgctg ccactgcgcc caggcttctt tggagatctc
    30961 gttatagatg cgttttccca gttcgccggg gtacagctga aaatcctgac cttctgcttc
    31021 acgttgcagg aaagtacaaa aaatcgttct gctcataaat catcctcttt atcgactcac
    31081 gcgctaaacc ggcgcgccag tgcgtaactg ctgtaacaaa cgctccacgg gagccgccag
    31141 gccaactgac ggcggttgcg ctaagttata ccagagcgca ttgtcttcat ccatgcagcc
    31201 ggtgaatgac gacacgggaa gccacatagg cacaatatct aagtggaaat ggctgaaggt
    31261 atgccgaaac gcggtcagct gcgtcaggtt atcggcagca atctgtcgtt gcgccagcca
    31321 ctgccgcaaa ctttcttcat cggcaaactg cgggaaacag tataaaccgc cccacaatcc
    31381 actcggcgga cgctgcgcca gcaatacttc atcttcgtgc tgtaacagca aaaagtagcc
    31441 ggtgcgctct ggcagcgtct gtttcggttt tttgcccgga taaagcgacc agctattgtt
    31501 ggcggcggca atacatccgt tttgtagcgg acagagcgaa catttcggct tagagcgcgt
    31561 acaaatcatc gcgcccaaat ccatcatcgc ctgattaaac cgttccacgc caaccgcggg
    31621 cgtcacctgc tcgctcaaac tccatagttt attctcgacc tcttttttcc cgggccagcc
    31681 gcttacagca tagcagcgcg ccagcacgcg tttgacgtta ccgtcgagaa tcggaaagtg
    31741 cttacccaga gaaagcgaga gaatcgcgcc tgcggtggaa cgcccgacgc ccggcagtgc
    31801 tgcaacttcc tcaaaggttt ccgggaattt accgccgtgt aaggccgcca cttgttgtgc
    31861 cgctttatgc agattgcgcg ctcgggcgta atagccaagc ccggtccaca agtggagaac
    31921 ttcgtcgagc ggcgcattgg cgagatcggt caccgttggg aagcgcacca taaagcgttc
    31981 aaaatagggg ataacggtcg caacctgagt ttgttgcaac atcacttctg agagccatac
    32041 tttgtagggc gtcttgtcaa tttgccaggg cagcgttttc cgcccgtatt tatcgtacca
    32101 gtccagaacc tgggctgaaa attgcgacgc ttgcatggtc accgaattca ctgttgttgg
    32161 gggcaagatt gcagcacagc gacggcaggg tgtaaaccgg aactttccgc agccacggcc
    32221 ttatcatgct tgcatccggc aactaacttt ggataatgcc cgttttcaga acactttcac
    32281 aagcgactaa atctttatga aaaacgacgt catctcaccg gaatttgatg aaaacggccg
    32341 cccgctgcgc cgtatccgta gttttgtgcg ccgccagggg cgactgacca aaggccagga
    32401 acatgcgctg gaaaactact ggccggtgat gggcgttgag ttcagcgaag atatgctgga
    32461 tttccctgcg ctttttggcc gtgaagcgcc ggtgacgctt gagattggtt ttggcatggg
    32521 cgcgtcgttg gtggcaatgg ctaaagatcg ccccgagcag gacttcctcg gcattgaagt
    32581 gcattcaccg ggcgttggtg cgtgcctggc ttctgcgcat gaagagggct taagcaacct
    32641 gcgcgtgatg tgtcacgatg cggttgaagt gctgcataaa atgattcctg acaattcatt
    32701 gcgcatggtg cagctctttt tccctgaccc gtggcacaaa gcgcgccata ataaacgccg
    32761 tatcgttcag gtgccgtttg ccgagctggt aaaaagcaaa ctgcagctgg ggggcatatt
    32821 ccatatggcg accgactggg aaccttatgc ggaacatatg cttgaagtga tgtcttctat
    32881 tgacggttat aaaaacctgt cagagagcaa tgattacgta ccgcgtccgg catcacgtcc
    32941 ggtgacgaaa tttgaacaac gtggtcatcg tcttggtcac ggagtatggg acttaatgtt
    33001 cgagagggtg aaataatggc aaagaaccgt agccgtcgtc tgcgtaaaaa aatgcacatc
    33061 gacgaattcc aggaattagg attttcggtg gcatggcgat tcccggaagg tacatcggaa
    33121 gaacagattg ataaaaccgt tgatgacttt attaacgagg ttatcgaacc gaacaaactg
    33181 gcctttgacg gcagcggtta tctggcctgg gaaggtctga tctgcatgca ggaaatcggc
    33241 aaatgcaccg aagaacatca ggcgattgtg cgtaagtggc tggaagaacg caaactggaa
    33301 gaggtacgca ccagcgaact tttcgacgtt tggtgggact aagaaagcat acgggcgatg
    33361 acaaatgcaa aactgcctga tgtgctacgc ttatcaggcc tataaagatg cacgatcgag
    33421 taggctggat aaggcgttta cgccgcatcc ggcatggaaa acgcgcactt tgttatcaat
    33481 ctggggccag caaatgctgg cctgatttgt tcttgaggga agactatgat gcgcaaaatg
    33541 ctgctggcgg cagcactttc agtgacggca atgaccgctc acgccgacta ccagtgcagc
    33601 gtcacgccgc gtgacgatgt gattgtcagc ccgcaaaccg tgcaggtgaa gggcgaaaac
    33661 ggcaatctgg tgatcacgcc agacggcaac gtgatgtata acggtaagca atattccctg
    33721 aatgctgccc agcgcgagca ggcgaaggat tatcaggctg aactacgtag caccctgccg
    33781 tggattgatg aaggcgcgaa aagccgcgtc gagaaagccc gtattgcgct ggataaaatt
    33841 atcgttcagg agatgggcga aagcagcaaa atgcgcagcc gtctgaccaa acttgatgcg
    33901 cagctgaaag agcagatgaa ccgcattatc gaaacgcgca gcgatggcct gacgtttcac
    33961 tataaagcca ttgatcaggt tcgcgccgaa ggccagcaat tagtgaatca ggcaatgggc
    34021 ggaattttac aggacagcat taatgaaatg ggcgcgaaag cggtgctgaa aagcggcggt
    34081 aacccattac agaacgtgct gggaagcctg ggcggtctgc aatcctcaat ccaaaccgag
    34141 tggaaaaagc aggaaaaaga tttccagcag tttggcaaag atgtttgtag ccgcgttgtg
    34201 actctggagg atagccgcaa agccctggtc gggaatttaa aataatcctc tattttaaga
    34261 cggcataata cttttttatg ccgtttaatt cttcgttttg ttacctgcct ctaactttgt
    34321 agatctccaa aatatattca tgttgtaaat tgtttaacgt caagtttccc atacaaagct
    34381 aagggataat gcgtagcgtt cacgtaactg gaggaatgaa atggagtttt tcaaaaagac
    34441 ggcacttgcc gcactggtta tgggttttag tggtgcagca ttggcattac ccaatatcac
    34501 cattttagca accggcggga ccattgccgg tggtggtgac tccgcaacca aatctaacta
    34561 cacagcgggt aaagttggcg tagaaaatct ggttaatgcg gtgccgcaac tgaaggacat
    34621 tgcgaacgtt aaaggcgagc aggtagtgaa tattggctcc caggacatga acgatgatgt
    34681 ctggctgaca ctggcgaaaa aaattaacac cgactgcgat aaaactgacg gcttcgtcat
    34741 tacccacggt accgacacga tggaagaaac cgcttacttc ctcgacctga cggtgaaatg
    34801 cgacaaaccg gtggtgatgg tcggtgcaat gcgcccgtcc acgtctatga gcgcagacgg
    34861 tccattcaac ctgtataacg cggtagtgac cgcagctgat aaagcctccg ctaatcgtgg
    34921 cgtactggta gtgatgaacg acaccgtact tgatggccgt gatgtcacca aaaccaacac
    34981 caccgacgta gcgaccttca agtctgttaa ctacggtcct ctgggttaca ttcacaacgg
    35041 taagattgac taccaacgta ccccggcacg taagcacacc agcgacacgc cgttcgatgt
    35101 ctctaagctg aatgaactgc cgaaagtcgg cattgtttat aactacgcta acgcatccga
    35161 tcttccggct aaagcactgg tagatgcggg ctatgatggc atcgttagcg ctggcgtggg
    35221 taacggcaac ctgtataaaa ccgtatttga cacccttgca accgctgcga aaaacggcac
    35281 tgcagtagtg cgttcttccc gcgtaccgac gggcgctacc actcaggatg ccgaagtgga
    35341 tgatgcgaaa tacggcttcg tcgcctctgg cacgttgaac ccgcaaaaag cgcgcgttct
    35401 gctgcaactg gctctgacgc aaactaaaga tccgcagcag atccagcaga tcttcaatca
    35461 gtactaatca cttcgccccg gtatcgtgcc ggggcttatt cacttcagac tcacgtccat
    35521 tgccaatttt tattactcta atgataatca ccggaataaa ttattccgcg cgagggtttt
    35581 cgggtgaaaa agcaatggat tgttggtacg gcgctgctta tgttgatgac tggtaatgtc
    35641 cgggcagatg gtgaaccgcc aactgaaaat atcttaaaag atcaattcaa aaagcagtat
    35701 cacggcattc tcaagcttga tgtcatcacc ttaaaaaatc ttgatgctaa gggtaatcag
    35761 gccacctggt cagtggaagg cgatgtctct tccagtgacg atctctatac ctgggtcggt
    35821 cagttggcag attacgagct gctcgaacag acctggacga aagataaacc ggtgaaattc
    35881 tcggcgatgt taaccagtaa aggaacgccc gcgtctggct ggtcggtgaa cttttactct
    35941 tttcaggcgg cagccagcga tcgtgggcgg gtggttgacg atatcaaaac gaataataaa
    36001 tatctgatcg tgaatagcga agatttcaat tatcgcttta gtcagcttga gtctgcgttg
    36061 aataaccaga ataactcgat tcctgcactg aaaaaggatg taaaagcgct tgataagcaa
    36121 atggttgcgg cgcagaaagc agcggatgca tattggggaa aagatgcaaa cggtaagcaa
    36181 aggacccgcg aagacgcctt taaaaaaatt caccaacagc gtgatgattt taataaacag
    36241 aacgatagcg aggcgttcgc ggttaaatat gacaaagagg tttatcaacc ggcgattgcg
    36301 gcatgtcata aacagagtga agagtgttat gaagtgccaa ttcagcagaa acgagatttt
    36361 gatatcaacg aacaacggcg acagactttt ctacaatcac aaaaactcag ccgtaaattg
    36421 caggatgact ggataacgct cgaaaaaggg caatatccgt taacgatgaa agtttcagaa
    36481 ataaacagta aaaaagtcac gattttgatg aaaattgacg atatcaacca ggttaatgaa
    36541 cgctggaaaa aagataccga acaattgcgg cgcaatgggg tgattaagta atttttgcga
    36601 tgacgcaaga gttgtagccg gatgttgcgt acacgccgca tccggcaata caaatttact
    36661 cagccagaaa aagctccagc agcgaattta aaaacaactt cccatgttcc gttatctgcc
    36721 agtaatccgc acattctgtg agataaccct gagcgatagc ctcgtctaac tgtgggcgaa
    36781 taacctcttc tgaaaggcca gtatactggc taaactccac gcgcggcgca ggttccagca
    36841 aacggaagcg attcataaag aactcaaatg gtttatctgc ggcttcgaca tcacgctggc
    36901 tttccagata ccgcccctgc ataaaaccac gtggatgacg cgttttggtg gtacgcagaa
    36961 tgcgcccatc cgggaaggtc actttgccat gcgcgccgca gccaataccg atgtagtcac
    37021 caaagcgcca gtagttgaga ttgtgctggc actgataacc gggtttggcg taagcggaag
    37081 tttcatattg ctgataaccc gctgcggtta ataactgatg cccctgttcg aaaatgtccc
    37141 acagcgcgtc atcgtccggc agtacaggag ggcgcgagcc aaacagcgta ttgggttcga
    37201 tggtcagttg ataccaggaa agatgcggcg gattcagttc aatagcctgg cgcagatcgc
    37261 ctagcgcctc ttccagtgat tgatccggca gcccatgcat caaatcgagg ttaaagctac
    37321 gtaaccctaa accgctcgcc agatttgccg ctcgtttcgc ttcttgcggg ccatgaatgc
    37381 gcccaagtcg tttcagcttt tcttcgctaa aactctgtac gccaatggag atgcggttca
    37441 caccagcacg ctgataatca acaaagcgat cggcttcaac cgtaccagga ttcgcttcca
    37501 tcgtaatttc tgcatccgct gccagcggca aacgcgcacg tacgccgtcc agcagcgttt
    37561 gcatcgccgg gccggaaagc aggctcggcg taccaccgcc aataaagatt gtctttactt
    37621 cacggccctg agcgtaagcc acatcgttgt ccagatcgtt aagcagatgc tgaacataat
    37681 cgtcgtgcgg cacttctcct ttcaacgcgt gagagttgaa atcgcagtac gggcatttct
    37741 gcacgcacca cgggatgtga atgtagagac tcagcggcgg taatttaacc attacgtaaa
    37801 gcgtccagca gcagtttcaa cgcctgacca cggtgggaaa tggcgctctt ttcttcgcgg
    37861 gtcagttcgg cagcggtttt cccttcggaa ggtacgaaga agattggatc ataaccaaag
    37921 ccaccagtgc ccgctggttc acgagtaatc acgcctggcc agctaccgtg gcataccagc
    37981 ggagtgggat cttccgcgtg acgcagatat accagcacgc agtggaaacg cgcctgacgt
    38041 tggtcgtccg gtacgtcttt cattgtttcc agcagttttt gcagattctt ttgatcggtc
    38101 gcgtcttcac cggaataacg cgcggagtaa atacccggcg cgccacccag cgcatctacc
    38161 gccagaccgg agtcgtcggc aattgccggt aaaccggtca cttttgccgc atggcgcgct
    38221 ttcagaatcg cgttttcgat aaaggtcagg ccggtttctt cagcggaatc tacgccgagg
    38281 tctgtttggg ccacgatatc aagaccgaag tcgctaagca gcgacgccag ctcacgcact
    38341 ttaccggcat tgccggttgc gaggacaact ttttgcatag gatacctaat taattaacgc
    38401 cgcgatttct ggcgggattt gttgcggatt aatgatttta atttgtttgt ggcggccaag
    38461 ttcgcctttt tcaatcacca cctggctttt ggcaacccgg aattgcttac cgagaaactt
    38521 caccagatgg ctgttggcct ggccgtcaac cggcggcgcg gtaatggcga cttttacctc
    38581 gtcgccatgt aaaccgacaa tagaatcacg gctggctttc ggctgaatat agagccgtaa
    38641 aaccagaccg tcatcattaa ctgttacggc actcataacg ccatccacag ccccggcagc
    38701 agcatatttc cggtcgcctg caagacttct gcgacaccca tattgatgac atacagcagc
    38761 agaacgagga tcatcggcga gaaatcaatc ccacccattg ccggtagcaa gcggcgaatc
    38821 gggcgcagca gcggatcggc cagctgaatc agcacgtatt caatcgggct acgaccctgg
    38881 cttacccagc tcataatcgc catcaccagc aggacccaga atatcagcag gccgatggtt
    38941 ttcagcagaa tcaataaacc ggcaatccaa atgattggca ggaaggtcac cactttaaac
    39001 agcacgatgg ctttaacgaa actgagaata taggcaacca gcagcgaggc gctatcaatg
    39061 ggtcccattg ccggaataac gcggcgcagt ggcccgataa tcggctgcgt taccttcact
    39121 acaaactgtg agaaggggtt gtaaaaatca caatgagccc actgcatcca gatgcgtaat
    39181 aacagcacca tggtatacag ctcaatgacc gttgaaagca ggaaagtcaa cgtattcatg
    39241 gcgttccttt aattccttat tttttagagt aatcacgcgc accaaaaatt gcagtgccga
    39301 tacgaaccat tgtgctacct gccgcaatgg cggcttccat atcgtccgac attcccagag
    39361 agagcgtgtc gatatgcggg tagcgcgttt tcagtccggc aaatgctaca gccatttggc
    39421 gtgcaacttc aaactgcctt acatattctg actcaggcgc agggattgcc atcagcccgc
    39481 gcagacgtaa acgcggtagt tcagcgaccg cagctgccag ctcgtccagt tcagccagtt
    39541 gaatcccgga cttactgttt tcatcactaa tgttaatttg aatcagtacg ttaagagggg
    39601 gaagttctgc cgggcgctgg tcgttcagac gggtagcgat gcgcaaacgg tcgatggtat
    39661 gacaccagtc gaaatgctct gccaccaggc ggcttttatt agactgcaac gggccaataa
    39721 aatgccattc taatcctgtt atgcccagtt cctgaaagtg gcgaatttta tctacccctt
    39781 cctgaacgta gttttcacca aattgacgct gcccggcatc aatggcttct gcgatggcgc
    39841 tcgcaggttt tgttttactg actgcaagca gcgtaatttc ttctggagaa cggccgcaac
    39901 gcgttgcagc cgctgagatt ttgtcccgga cctgtgccag gttatgcgca atatcgttca
    39961 ttttccgagg atcttagtat gaatatggaa gaaattgtgg cccttagtgt aaagcataac
    40021 gtctcggatc tacacctgtg cagcgcctgg cccgcacgat ggcgtattcg cgggagaatg
    40081 gaagctgcgc cgtttgacgc gccggacgtc gaagagctac tgcgggagtg gctggatgac
    40141 gatcagcggg caatattgct ggagaatggt cagctggatt ttgctgtgtc gctggcggaa
    40201 aaccagcgat tgcgcggcag tgcgttcgca caacggcaag gtatttctct ggcgttacgg
    40261 ctgttacctt cgcactgccc gcagctcgaa cagcttggcg caccaccggt attgccggaa
    40321 ttactcaaga gcgagaatgg cctgattctg gtgacggggg cgacggggag cggcaaatct
    40381 accacgctgg cggcgatggt tggctatctc aatcaacatg ccgatgcgca tattctgacg
    40441 ctggaagatc ctgtggaata tctctatacc agtcagcgat gtttgatcca gcagcgggag
    40501 attggtttgc actgtatgac tttcgcatcg ggattgcggg ctgcattgcg ggaagatcct
    40561 gatgtgattt tgctcggaga gctgcgtgat agcgagacaa tccgtctggc gctgacggcg
    40621 gcagaaaccg ggcatctggt gctggcgaca ttacacacgc gcggcgcagc gcaggcagtt
    40681 gagcgactgg tggattcatt tcctgcgcag gaaaaagatc ccgtgcgtaa tcaactggca
    40741 ggtagtttac gggcagtgtt gtcacaaaaa ctggaagtgg ataaacagga aggacgcgtg
    40801 gcgctgtttg aattactgat taacacaccc gcggtgggga atttgattcg tgaagggaaa
    40861 acccaccagt taccgcatgt tattcaaacc gggcagcagg tggggatgat aacgtttcag
    40921 cagagttatc agcagcgggt gaaagaaggg cgcttgtgac gttgctgtgt ctgatacgaa
    40981 ttgtaaatat gcccgatgcg caagcttatc gggcctacaa gggaagcgta actttgaatt
    41041 cgcactcact gtaggttgga taaggcgttt acgccgcatc caacatgaaa caacgagcac
    41101 attgtcagaa atctgtgcgc tagtaaatcg ccttaatatc cctgctcgaa atagctttcg
    41161 agaataataa ccgcagaggc agagtcgact ttgcctttgt tgagcgcccg atagccgccc
    41221 tgttcaaaca gaccggaacg tgcctccacg gtgctaagac gctcgtcatg gagctttact
    41281 tcaacaccga aacgaccatg aacacggttg gcaaatttac gcgctctggc ggttaacggc
    41341 tgttcggtgc cgtccatatt cagcggcaaa ccgacgatga tttcgtccgg ctgccactct
    41401 ttcagtaaac gctcgataat gttccagtcc ggcgtaccgt cctgcgcttt aattgcaggc
    41461 aaagggcgag cggtgccggt aatgcgttgg ccgaccgcta cgccaatgct tttggtgccg
    41521 aagtcgaagg cgagtaaggt tccactcatc aggcgtgtcc tgccacacca ggcatggtga
    41581 gaatatccac accaatcagt tttgccgcct cgcgccagcg gtccgcaatc ggcgttttga
    41641 acagaatatt cagatctgcc ggggccgtta gccacgcgtt atcgagaatt tcttgttcca
    41701 gttgaccttt ctcccaggag gcataaccca gagccaccaa tacgtcagac ggttgtttat
    41761 cggtgccgag cgtttccagc acatcgcggg aagtggtcat cactgtgttg tctgaaatgc
    41821 gaatgctgga agcaaaattg gagggcggag tatgcaaaat aaacccgcga tcttcagcca
    41881 gcggaccgcc gagcataacc ggtttatcca gacggattga ttcatcacgc ggctccggcg
    41941 tgatcttcag cttttccaga atcccttcaa ttttgagatt ttccagcggc ttgttgacga
    42001 tgatccccat tgcaccattg gtattatgtt cgcaaatgta gaccacggaa cgacggaaaa
    42061 tcggatcctg gagagcaggc atggcaataa gaaagtgatg ctgtaaattc attgtcagag
    42121 gttctgtttc ctggttcaaa aaagcaacag cgcccagtat gcgtggaaag aagggcgctg
    42181 tcactcagag tctcaacgag atccttctcg ctaaggtggg ttactgctgc tgtaaacgtg
    42241 cttcgatggc atccattaac attccggtaa tcgacaccgg aaactctgct tcaatctcac
    42301 gaatacaggt tgggctggtg acgttaattt cagtcagacg gtcgccgatg atatccagac
    42361 caacaaaaat cagccctttt tctttcagcg tcggcccaat ctgacgggcg attttccagt
    42421 cactttccgt cagcggacga ggttcaccgc gaccaccggc agccagattg ccacgggttt
    42481 cgcccccctg cggaatacgc gccaggcaat acggcaccgg ctcgccatcc actaccagca
    42541 cgcgtttgtc gccatcttta atggctggca ggtaattttg cgccatgcag tagcgagtgc
    42601 catgctcagt cagggtttcg gcaatcacgc cgaggtttgg atcgccttct ttcacgcgga
    42661 aaatcgacgc gccgcccata ccgtccagcg gcttaagaat gatgtcgctg tgtttttccc
    42721 agaaagcttt tagctgcgct ttattgcgtg tgaccagcgt ttctggtgtt aagtcagaga
    42781 accaggcagt gaacagtttt tcgttacaat cgcgcaggct ctgcggcttg ttaacgatca
    42841 gcgtcccttt atcttcggca cgttccagaa tataggtcgc gtagataaac tcggtatcaa
    42901 acggcgggtc tttacgcatc aggatcacat cgagatcggc cagcggcaga tcctgctcac
    42961 ccgtgaactc gtaccattta tcgtagttct gttcgacgct caaagtacgg gtacgagcgc
    43021 gagcctcacc attgatcaaa tagagatcgg ccatctccat atagtgaagt tcgtaaccac
    43081 gacgctgtgc ttccagcaac atagcgaaac tggaatcttt cttgatgttg atgtttgcga
    43141 tggggtccat cacgatgccg agcttgatca ttattcttct ccgttagccc aaatcgccaa
    43201 aacgcacttg tagcgcggta atggcggtga gcgcagttgt ctcagtacgc aaaacgcgag
    43261 gtcccaacag gatatcagta aattgatagc gggcagtcat ggcaatttca tctgccgata
    43321 aaccgccttc cgggccaatc agcaggcgga cgcgttcgac cggtaacggc aacgtattga
    43381 tgctgttact ggcgcgcggg tgaagattca gtttcagtcc ttcatcctgc tctgcacacc
    43441 aggcttccag atccatcgct ggacggattt ccggcacccg gttacggcca cactgctcac
    43501 aggcagcaat tgcaatcttc tgccactgct ggagcttctt gttcagacgt tcactatcca
    43561 gtttaacgcc gcagcgctca gaaaaaagtg gcgtaatgag gcttacaccg agttcgatcg
    43621 atttctggat agtaaattcc attttttcac cacgcgacat cacctgaccg aggtgaatat
    43681 gcagcggaga ttcgcgatcg tcgatctggc cttccagcac cttcacttcc acgctttttt
    43741 tgctggcgct ggtaatttcg gcgtcaaaga cttggttgct gccgtcaaac aattgcagcg
    43801 cctgtcccgg ccccatacgc agtacgcgcc cgatatggtt ggcggcatct tcgcaaagcg
    43861 cgatgtgaga atggctggtc agtggttcag gatgataaat gcgggggata cgcatagtta
    43921 aaaatccgcg tcgtctcccc acgcggttgt acgcgtgggg taggggttaa caaaaagaat
    43981 cccgctagtg taggttagct ctttcgcgcc tggcaagcgc gttgcacata cgggttatga
    44041 ttgccctgca ccttcgcgat gcgttcatca cgctcgcact ccctgtcggt aactggatac
    44101 atcttgtccc atgcgttgaa cagctgcgtt tgctgacgag agagtgtcag gttgtattgg
    44161 tcacgcatat agaagtaggt gcgcgcaatg gcaccgcgtg cacgcgctgg tggttcggca
    44221 actttttctt tgaaatcgac cttcatggcg cattgaccgt actggccttc accgccattc
    44281 cactggctgt acataaagtt gccgcgatcg ccattcacct caccgactga cggctgcagg
    44341 ttatgcatat cgctttccat cttgcgatag accggatctt tggcgcaatt tttacgtcca
    44401 ccgtcctgcc agcactggcg ctggtgaccg aattgccagg caggaacgac gtgttcccac
    44461 tctacgcggc tggcacggtt ttcatttttt cgcacctgat agccgcacga ttgcagatca
    44521 acaacgcctt ttttgccctg ccagttaatt ttacatccgc aataaaacgt accgggcgcg
    44581 tcagcgtgga cttttaccgc cgcggctttc gcttgagaaa aactattgat accttcggcc
    44641 aatgccgggc cggaaaatgc tgtgctcagt accaccgcag caatagacaa ataacggtac
    44701 atcacttact ccgtgttaaa acgagccagc aacgtagcga aagttgtttt ggtatgcaat
    44761 cagccagatc aggaaagttc ctgatagttc aggtggttat ttcgcaacca gctgttcgcc
    44821 gcagtgaaca cagcgataga cggcttcgcc acgaactacg cgattatgac ggcgtacggt
    44881 aagctgatgc tcctggcact tgcagcggta ggggaaggtg ttgcggcgca cggattgcag
    44941 ttcgaactga tgcgtacgac gggcgggaac gcccagcacg ctttccatca tccacttcca
    45001 ctctttgcca tgtggcgcta cgcgcccgaa atgtttccat accagcaaat gtgccagttc
    45061 gtgtggcact acctcttcaa taaaggcttc actgttttcc aacagcaaaa cgggattgag
    45121 gcgaatttca tagctttcca gccaggctgt tccggcagag gttccgcgct gggtgtaaga
    45181 gagttttggc tccgggtagt tacgccctag cttcaggttg gcctgggcga gtttttcccg
    45241 caggcgacgc ataacggcct gttggatggc gatagggaga cgggatgttt tcatagcggc
    45301 agaggataga gcgaagaaaa gatgactgca agaggtggct tcctccgcga cgggaggaag
    45361 cctgaggaga ttaatcgtga gcgccgattt cgcgcagttt acgacctttc atcagattac
    45421 gttcaatatg ttccagcgaa acatgtttgg tttccggtac cagccacaat gtcagcagga
    45481 taaacagtac attcagagca gcatacaccc agaaggtgtt ggcgttaccc agcgtgttga
    45541 gcatggtcag gaacgttgcg ccgacaatca tgttggcaat ccagttggtg gcggtggaac
    45601 aggtgatgcc aaaatcacgg cctttcagcg gctgaatttc agagcacagt acccaaatca
    45661 gcggacccgc gctcatggca aaaccgacaa taaacatcag cagcatggcg atggcgaaat
    45721 actgcgccga cggagagtgg ataccgatat gcatcattgt accgagtacg cccataccag
    45781 cagccatcac caggaagccc agcgttagcg ttggtttacg tccccagcgg tcaacaaggc
    45841 cgattgcgat aaaggtggca agtacgttgg tcaggccgac aatcactgtc ccccacattt
    45901 gctcggtggt gttggtataa cccgccagtt cgaagatttt cggcgcgtaa tacatgatga
    45961 cgttcatccc ggtgaattgc tgcattacct gcaatagtac gccaaggaac accgcgcggc
    46021 ggaagttgct gttctcttta aacagcgccc agccactctg tttaacctgc aaactttcac
    46081 ggatttcatc cagttcgcgt ttcgcttccg cgctggtgtc acgcaggcgt agcagcacgc
    46141 gttcggcatc aacaaaacgg cgtttggcgg caaaccaacg tgggctgtct ggcaggaaga
    46201 agacaccaat cagcagcaaa attgccggga tgataatcac acccagcatc cagcgccatg
    46261 caccggtgta gctgaaggcg gtatcagaaa gataagcgcc gaggatcccg atagtgatca
    46321 tcaactgata catcgagatc atactgccgc gaattttttc cggcgcaatt tctgacaggt
    46381 acagtggtgc ggtataagag gccacaccca ccgccagccc cagcagaacg cgggaaagaa
    46441 tcagtacttc aacgtttggc gcagccgcag agaacagtga accggcaaca aacaggattg
    46501 cgccgatcat caggctcttt ttgcgcccga gtttaaagga gagccagccg ctgcccaccg
    46561 caccgactgc cgcaccgaac atcatggagc ttacgaccca ttcttgcgtg tgcgaagtaa
    46621 tctggaattc atctgcaata aacggcagtg cgccagcaat tacaccgata tccaggccaa
    46681 agagtaatcc cgccagagcg gcaaggaagc agacgaaaaa cgtcattgcc ttgtttgacc
    46741 gcccctgttt tttagcgtca ggcatgatgc cctccaatat ggttattttt tattgtgaat
    46801 taagataggt gagtacgacg taaaaagatg tgaagcaaat cacatcagtg taatcgctta
    46861 cactaacatt tatcttagtt atgatttaag tgcatgaaaa taattgagtt atatcatcat
    46921 agattaaagg ttgttatctg caattaagac tttactgaaa agaaatgtaa caactgtgaa
    46981 ggctgaataa taatatgaaa atattacaaa tatttggctg gcgtggttgg tgtaatcggt
    47041 ttcagtagtg atgataattt gcggtaaaac gtgttgagga agaatgatgt tattgtgcgg
    47101 tcggtttttt tgccggatgc ggcgtgaatg cctgtccacc gcggtgaggt aaaagaaaaa
    47161 ggccagcgcg aggctggcct ttgaaagcag gtgaagaaag attacttcag accggcagca
    47221 tcacgcagca gctgcgcttt gtcggttttt tcccacggga aatgttcacg accaaagtga
    47281 ccgtatgctg cggtttcttt gtagatcggg tgcagcagat ccagcatctg aatcagaccg
    47341 tatgggcgca ggtcgaagaa ttcacgtacc agcagagtca gttgttcaga aggcactttc
    47401 tcagtaccga aagtttctac catgatggaa gtcggttcag ccacgccgat tgcgtaggaa
    47461 acctgaattt cacaacgatc ggccaggcca gcagcaacaa tgtttttcgc gacataacgt
    47521 gctgcgtagg ctgcggaacg gtccactttt gatggatctt taccggagaa tgcaccgcca
    47581 ccatgacgcg ccatgccgcc gtaggtatca acgatgattt tacgaccagt cagaccgcag
    47641 tcacccatcg ggccaccgat aacgaaacga ccggtcgggt tgatgaagaa tttggtggca
    47701 gaagtcagcc attcagcggg cagaatcggc ttgatgatct cttccattac cgcttcttgc
    47761 agcgatttct ggtcgatctc ttcagagtgc tgagtggaaa gcacgacagc atcaatgcca
    47821 acgattttgc cgtcgtcata ctggaaagtc acctggcttt tcgcgtccgg gcgcagccac
    47881 ggcagagtgc cgtttttacg cacttcagcc tgacgctgta ccagacgatg tgcataagtg
    47941 ataggtgctg gcatcagcac gtcggtttca ttagttgcgt agccaaacat caggccctgg
    48001 tcgcccgcgc cctgttccag cggatcggca cggtcaacgc cctggttgat gtcaggagac
    48061 tgtttaccga tggcgctcag aaccgcacag gagttagcgt caaagcccat gtcggaatgt
    48121 acatagccaa tttcgcgaac ggtgttacgg gtgatctctt cgatgtctac ccaggcgctg
    48181 gtggtgattt cgccgccaac taaaaccatg ccggttttta cgtaggtttc gcaagcaacg
    48241 cgtgctttcg gatcctgttc gaggatcgcg tctaaaacgg catcagagat ttggtcagca
    48301 attttgtcag gatgcccttc agagacggac tcggacgtaa aaaggtgttt tgccatattt
    48361 aatatcacct aaagagaatt tggttagctc aaactgttgt gtggattttc tgtggtagcg
    48421 gatcctacca cgactctgca ggttaaaaac actggcagtc tgagtgttaa tcggtatgga
    48481 tggattaaca tctggatggc tattttaggt cagttcttca cccaatttcc actttttttt
    48541 gaatcgtgtc tcattctgtt aaaaacgtgg ctggaaattt ttcctgacaa tgctggcatt
    48601 ctgcgtattt atcttttgca attttctgcc attgtggggt ataaaacgcg gcgcgcggct
    48661 taaataaaaa gcacacgacg tttctttcgt gttgccactt ccagccgggt tgaaaacaga
    48721 gttttggctt gtgggttcgt cttaacaggc ggccgtggag gtgatacgaa ataatgaacc
    48781 gttgtctgct gcttaacctg tctcaccgtt ctggtgaaga ttcgttcccc gcactctgca
    48841 tctctgcttt gcatacctgc cgatgttata cccatctcgg cgcttctcag gattcaagag
    48901 ctggttacag ctactgagga ctgaacaagg gcgctcttgt aaaaacaaga gttttctcgt
    48961 ggtttcgccg aactgtcaca cttacgttcg gttatgtgct taataatgtt atgaaaaaga
    49021 aaccggttgc gcagttggag cgtcagcatt cactgctgga aaatccatgt gcttatgggt
    49081 tgttatcgca gttccaggct gcgatagtcg ttaactgttt tacacttaat aaaataattt
    49141 gaggttcgct atgtctgacg acatgtctat gggtttgcct tcgtcagcgg gcgaacacgg
    49201 tgtactacgc tccatgcagg aggttgcaat gagctcccag gaagccagca agatgctgcg
    49261 tacttacaat attgcctggt ggggcaataa ctactatgac gttaacgagc tgggccacat
    49321 cagcgtgtgc ccggacccgg acgtcccgga agctcgcgtc gatctcgcgc agttagtgaa
    49381 aactcgcgaa gcacagggtc agcgtctgcc tgcactgttc tgtttcccac agatcctgca
    49441 gcaccgtttg cgttccatta acgccgcgtt caaacgagcg cgggaatcct acggctataa
    49501 cggcgattac ttcctcgttt atccgatcaa agttaaccag caccgccgcg tgattgagtc
    49561 cctgattcat tcgggcgaac cgctgggcct ggaagccggt tccaaagctg agttgatggc
    49621 ggtgctggca catgctggca tgacccgtag cgtcatcgtc tgcaacggtt ataaggaccg
    49681 cgaatatatc cgcctggcat taattggcga gaagatgggc cacaaggtct atctggtcat
    49741 tgagaagatg tcagaaattg ccattgtgct ggatgaagca gaacgtctga atgtcgttcc
    49801 tcgtctgggc gtgcgtgcac gtctggcgtc gcaaggctcg ggtaaatggc agtcctccgg
    49861 cggggaaaaa tcgaagtttg gcctggcggc gactcaagta ctgcaactgg ttgaaacgct
    49921 gcgtgaagcc gggcgtctcg acagcctgca actgctgcac ttccacctcg gttcgcagat
    49981 ggcgaatatt cgcgatatcg cgacaggcgt tcgtgaatcc gcgcgtttct atgtggaact
    50041 gcacaagctg ggcgtcaata ttcagtgctt cgacgtcggc ggcggtctgg gcgtggacta
    50101 tgaaggcacc cgttcgcagt ctgactgctc cgtaaactac ggcctcaatg aatacgccaa
    50161 caacatcatc tgggcgattg gtgatgcgtg tgaagaaaat ggtctgccac atccgacggt
    50221 aatcaccgaa tcgggtcgtg cggtgactgc gcatcacacc gtgttggtgt cgaatatcat
    50281 cggcgtggaa cgtaacgaat acacggtgcc gaccgcgcct gcagaagacg caccgcgcgc
    50341 cctgcaaagc atgtgggaaa cctggcagga aatgcacgaa ccgggaactc gccgttctct
    50401 gcgtgaatgg ttacacgaca gccagatgga tctgcacgac attcatatcg gctactcttc
    50461 cggcaccttt agcctgcaag aacgcgcatg ggctgagcag ctttatttga gcatgtgcca
    50521 tgaagtgcaa aagcagctgg atccgcaaaa ccgtgctcat cgtccgatta tcgacgagct
    50581 gcaggaacgt atggcggaca aaatgtacgt caacttctcg ctgttccagt cgatgccgga
    50641 tgcgtggggg atcgaccagt tgttcccggt tctgccgctg gaagggctgg atcaagtgcc
    50701 ggaacgccgc gctgtgctgc tggatattac ctgtgactca gacggtgcta tcgaccacta
    50761 tattgatggt gacggtattg ccacgacaat gcctatgccg gagtacgatc cggagaatcc
    50821 gccgatgctc ggtttcttta tggtcggcgc atatcaggag atcctcggca acatgcacaa
    50881 cctgttcggt gataccgaag cggttgacgt gttcgtcttc cctgacggca gcgtagaagt
    50941 ggaactgtct gacgaaggcg ataccgtggc ggacatgctg caatatgtgc agctcgatcc
    51001 gaaaacgctg ttaacccagt tccgcgatca ggtgaagaaa accgatcttg atgctgaact
    51061 gcaacaacag ttccttgaag agttcgaggc aggtttgtac ggttatacct atcttgaaga
    51121 tgaataatta agattattcc gctggcgggg cgatttattt acgtaaatcg gcccgctttc
    51181 tttttatata ttcttaaaaa taatttagct gcttttctta ttaaataatc tccctacaat
    51241 cgctgcatta attaaatgca ggggaacggt tatgaaaaaa tggaaagtac gtagcgcgtt
    51301 ggtggcatta atcgtgctgc tggccggttg ttccagtaat gcgcagtata atagcagtgc
    51361 atcgggtaat gtcggcaccg cctggggtgg ggatgtgcac tcaacggttc agggcgtatc
    51421 tgctgaacga gcttggcgcg atccggcaga gatgatcatt attagttatt cgactaatgt
    51481 tccttccggc tacgaccgcg tttatagcat tcgtattaac gaactggaat atgctattcg
    51541 cgatggtaat tttaaatctc taccaattac gcgggtttat gattcatcca ataatgaacc
    51601 acgctatatt gtccatgcca gagtcggaat gaattatcaa ttatatgtcc gtaattacag
    51661 tcgtaacacc aactacgaga tcgttgctac ggttgatggc ctggatgttc tgaatggtaa
    51721 acaaggttcg ctgaataaca acggttatat tgtgaatgcc ggagattcgt tagcgattaa
    51781 aggattccgc aaagataaac ataccgaagc ggcttttcag ttcgccaacg ttgctgattc
    51841 ctacgcagcc aattcggcgc aaggcgatgt gcgtaatacc ggtgtaatcg gctttgtcgc
    51901 ctttgaatta caaggcccgg cgcaaaacgc gctgccgccg tgctctggac aagcgtttcc
    51961 ggcagataac aatggctatg cgccgccacc gtgtcggaaa tagtaacctt gcttgatgcc
    52021 ttgcccattt agggcgataa ttagcacaaa cttgtgtttc gcaaatccaa cccttcctcg
    52081 tcgggcctaa cgacgcggag gggttttttt atatcgactt tgtaatagga gtccatccat
    52141 gagcacctta ggtcatcaat acgataactc actggtttcc aacgcctttg gttttttacg
    52201 cctgccgatg aacttccagc cgtatgacag cgatgctgac tgggtgatta ctggcgtgcc
    52261 gttcgatatg gccacttctg gtcgtgcggg tggtcgccac ggtccggcag cgatccgtca
    52321 ggtttcgacg aatctggcct gggaacacaa tcgcttcccg tggaatttcg acatgcgtga
    52381 gcgcctgaac gtcgtggact gcggcgatct ggtatatgcc ttcggagatg cccgtgagat
    52441 gagcgagaag ctgcaggcgc acgccgagaa gctgctggct gccggtaagc gtatgctctc
    52501 cttcggtggt gaccactttg ttacgctgcc gctgctgcgc gctcatgcga agcatttcgg
    52561 caaaatggcg ttggtacact ttgatgccca caccgatacc tacgctaacg gttgtgaatt
    52621 tgaccacggc accatgttct ataccgcgcc gaaagaaggt ctgatcgacc cgaatcattc
    52681 tgtgcagatt ggtattcgta ccgagtttga taaagacaac ggctttaccg tgctggacgc
    52741 ctgccaggtg aacgatcgca gcgtggatga cattatcgcc caggtgaaac agattgtggg
    52801 tgatatgccg gtttacctga cctttgatat cgactgcctg gatcctgctt ttgcaccagg
    52861 caccggtacg ccggtaattg gcggcctgac ctccgatcgc gccattaaac tggtacgcgg
    52921 cttgaaagat ctcaacatcg ttgggatgga cgtagtggag gtggctccgg catacgatca
    52981 gtcggaaatc accgctctgg ctgcggcgac gctggcgctg gagatgctgt atatccaggc
    53041 ggcgaaaaag ggcgagtaag cgccagataa gaggcgcgcg ggtgatgtgc cattaatgtc
    53101 ggatgcggcg tgaacgcctt atccgaccta cactcggcac ccgtaggccg gataagatac
    53161 gccagcatcg catccggcaa tgcgcacgga gtaacaaatg tgccattctt gtcagatgcg
    53221 gcgtgaacgc cttatccgac ctacgttcgg cactacctgg ctttacttaa ctccatccgc
    53281 gctcatacga tcgcgaatat gctgggcgcg ttcggcggat gcaggatggt cgtcaaacat
    53341 tgagctttgg cggccttctt ctagtttcgc cagtttttca aagctggtgg caagacctgc
    53401 cgggctgata ccgcgttggc gcagcaaatc gtaggagtaa tcatcggcct ctgattcctg
    53461 acgttgggta aattgcgaat tgactagctt ttcgcccagg tctcccagtt gtgattgtga
    53521 caaactgccg acaattccgc ctgcagaggc cgccgcaacg cgaattgcgt ttgtaccaag
    53581 tgctacctgc attcctttct tcacatggcc taatgccacg tgccccattt cgtgaccgag
    53641 caccgcttcg acttcgttat ccgtcatcat atccatcagc ccgctataga cgcggataca
    53701 gccgttagcc attgcaaagg cgttcacatc cttcgccata tacactttgt aattcaccgg
    53761 ctgaccgttg atgttatcgc ctaacgcgcg agaaattgtc gtcagacgtc tagcatattc
    53821 gctattggct ggcgcaatcg tcgctttgct gtccatctcc tggcatgcct gatcgctcag
    53881 ttttttcacc tgcgcatcac tcaaactgta agcctgaaaa gcttccgctc ctgatgagag
    53941 cagtccactg gagtccatat tctggcaacc ggtcagcacc gtagccacgc tcattgctac
    54001 caataaggcg cgaattttca tgttttctct tccgcacaag gtcaggatta ttctaaaaat
    54061 gcgccgtttg caggtgaatc gacgctcagt ctcagtataa ggaaaagcgc agcggacggg
    54121 cgagtggatt gcgcaacatg cgagcatgat ccagagattt ctgaagcggc aaaaggatgt
    54181 tccatgtaca tgacgagcgg cttgcggtaa attgttggca aattttccgg cgtagcccaa
    54241 aacgcgctgt cgtcaagtcg ttaagggcgt gcccttcatc atccgatctg gagtcaaaat
    54301 gtcctcacgt aaagagcttg ccaatgctat tcgtgcgctg agcatggacg cagtacagaa
    54361 agccaaatcc ggtcacccgg gtgcccctat gggtatggct gacattgccg aagtcctgtg
    54421 gcgtgatttc ctgaaacaca acccgcagaa tccgtcctgg gctgaccgtg accgcttcgt
    54481 gctgtccaac ggccacggct ccatgctgat ctacagcctg ctgcacctca ccggttacga
    54541 tctgccgatg gaagaactga aaaacttccg tcagctgcac tctaaaactc cgggtcaccc
    54601 ggaagtgggt tacaccgcag gtgtagaaac caccaccggt ccgctgggtc agggtattgc
    54661 caacgcagtc ggtatggcga ttgcagaaaa aacgctggcg gcgcagttta accgtccggg
    54721 ccacgacatc gtcgaccact acacctacgc cttcatgggc gacggctgca tgatggaagg
    54781 catctcccac gaagtttgct ctctggcggg tacgctgaag ctgggtaaac tgattgcgtt
    54841 ctacgatgac aacggtattt ctatcgatgg tcacgttgaa ggctggttta ccgacgacac
    54901 cgcaatgcgt ttcgaagctt acggctggca cgttattcgc gatatcgacg gtcatgacgc
    54961 ggcatccatc aaacgcgcag tagaagaagc gcgcgcagtg actgacaaac cgtccctgct
    55021 gatgtgcaaa accatcatcg gtttcggttc cccgaacaaa gccggtaccc acgactccca
    55081 cggtgcgccg ctgggcgacg ctgaaattgc cctgacccgc gaacagctgg gctggaaata
    55141 cgcgccgttc gaaatcccgt ctgaaatcta tgctcagtgg gatgcgaaag aagcaggcca
    55201 ggcgaaagaa tccgcatgga acgagaaatt cgctgcttac gcgaaagctt atccgcagga
    55261 agccgctgaa tttacccgcc gtatgaaagg cgaaatgccg tctgacttcg acgccaaagc
    55321 gaaagagttc atcgctaaac ttcaggctaa tccggcgaag atcgccagcc gtaaagcgtc
    55381 tcagaatgct atcgaagcgt tcgggccgct gttgccggaa ttcctcggcg gttctgcaga
    55441 cctggcgccg tctaacctga ccctgtggtc tggttctaaa gcaatcaacg aagatgctgc
    55501 gggtaactac atccactacg gtgtacgtga attcggtatg actgcgattg ccaacggtat
    55561 ctccctgcac ggtggtttcc tgccgtatac ctcaaccttc ctgatgttcg tggaatacgc
    55621 acgtaatgcc gtacgtatgg ctgcgctgat gaaacagcgt caggtgatgg tttacaccca
    55681 cgactccatc ggtctgggcg aagatggccc gactcaccag ccggtagaac agatcgcttc
    55741 tctgcgcgtg accccgaaca tgtctacatg gcgtccgtgt gaccaggttg aatccgcagt
    55801 agcgtggaaa tacggcgttg agcgtcagga cggcccgact gcgcttatcc tctcccgtca
    55861 gaacctggcg cagcaggaac gtactgaaga gcagctggca aacatcgcgc gcggtggtta
    55921 tgtgctgaaa gactgtgctg gtcagccgga actgatcttc atcgcgaccg gttctgaagt
    55981 tgaactggct gttgccgcct acgaaaaact gactgccgaa ggcgtgaaag cgcgcgtggt
    56041 ttccatgccg tctaccgacg cattcgacaa gcaggacgcg gcttaccgtg aatccgtact
    56101 gccgaaagcg gttaccgctc gtgttgcggt ggaagctggc attgctgact actggtacaa
    56161 gtatgttggc ctgaacggtg ctatcgtcgg catgaccacc ttcggtgaat ctgctccggc
    56221 agagcagctg tttgaagagt tcggcttcac cgttgacaac gttgttgcga aagcaaaagc
    56281 gctgctgtaa tcggtaagat gctttgtgag aagggccgca aatgcggccc ttcagattgt
    56341 tggcaaagtg cgcgttgttt atgccggatg cggcgtgaac gccttatccg tcctacaaga
    56401 ttttgcaaat tcaacatatt gcaggaagta tgtaggcctg ataagcgtag cgcatcaggc
    56461 agttttgtct ttgctatcag tctcaagggc cgcgaaagcg gccctttttt acttagtaga
    56521 gataaacgga actgtgctgc cggggatcat ggtatcgggc agtttaccgt cccaacgctc
    56581 agcagtggtg agggcaacaa gtccgggatt ctcccgcaga gcttcaccgc gcaggcggat
    56641 agcttctgct tcagccgcac tttttaaacg aatagtttca gcctctgccg cgcctcttac
    56701 gcgaatagtt tccgcttctg ctttggctgc cgctagttta ctgtctgctt ccgcttgggc
    56761 ctgagttacc gctatttgcg cctggatttt ctccgtttcc agattttgct tacgcgtggc
    56821 aatggcgact tctgctttca ttcggtcttc aatcgacttt tcataagcgt cagaaaaatc
    56881 aatattttca atctggaccc cgtcaattac cactggccca acaacggctt tgcgcatggc
    56941 attttgcaga tcctgaacca gttttgtacg atcctgtacg gcgctgatgg cggtatattg
    57001 tccgaaaacg ttctccagtt gagttggcaa ctgacggaca atcagacgat cttttaatgc
    57061 ttcaatggtg ttgtaggtgg tataaaccgc gcctgcttct gaaggtttaa tgtggaagct
    57121 gactgatact gtcatttgtg ccggttgctg gtcgcggctg taggcctgta atccctgata
    57181 gaccacagcc tgattacgcg tggaaatttt ctctacgctt tccataaaag ggatcttaaa
    57241 acccagcccc ggctcggcaa cttttacgat ttttccgtag cgcagcaaaa tgccgcgttc
    57301 accttcatta acggtgtaat acgagagaaa tggcagcaca accacggcaa gcacgccaat
    57361 ggcgatagca agagattttt gtgggcgaaa tgaggttaat gaaacaggtg ctttcatatg
    57421 aatcttcctt gagtaaagta taggaatatg tcacatgggg tgatgagaaa acagacaaca
    57481 gccgataatt cagaatttat aaaggggatg agcgaaaaaa taataaacgt aataagtatg
    57541 ttttcgaaca ttcaataaga aaatagagat gtgtatcaca aatatagtga tgaataattt
    57601 gttgattttg tagtttactt aacagattta taattgactt tttaaaagct tatttcatgt
    57661 tgttgttatt taatattttg ttatttttgc tcgtggagtt tctcctttca tgtgtccaat
    57721 tgctttcgtt aatttgtacg cgtattttat ttttaccgcc ggactgtacc ctaaaaataa
    57781 aataaggata atttatgcgg cttattgatt attttccaga gtcatcaatc tcagttatac
    57841 attcagcaaa agattggcag gaagctatcg atttctcgat ggtatcattg ctggataaaa
    57901 actatatcag cgagaattac attcaggcaa ttaaaggttc caccatcagc aatggtcctt
    57961 attatattat cgcaccaggc gtggcaatgc ctcatgcgcg accggaatgt ggagcgctta
    58021 aaaccgggat gtctttgacg ttacttaaac aaggcgttta ttttccgggg aatgacgaac
    58081 cgattaaatt actcatcgga ctttctgctg ccgatgccga ttcgcacatt ggtgctattc
    58141 aggcgttaag tgaattactg tgcgaagaag aaatactcga acaactctta accgcatcat
    58201 cagaaaaaca attagcggac attatcagcc gcggataatg actctctcct ctacaggata
    58261 atattatgga aaacaagtct gctcgtgcaa aggtccaggc ttttggggga tttttgactg
    58321 caatggtcat ccccaatatc ggtgctttta ttgcctgggg atttataacg gcattattta
    58381 ttcccaccgg ttggctgccg aatgaacatt tcgccaaaat tgttggtccg atgattacct
    58441 atttattgcc cgtgatgatt ggttctaccg gtggtcatct ggtcggcggt aaacgcggcg
    58501 cggtcatggg cggaattggt acgattggtg tgatcgtcgg cgctgaaatc ccgatgttcc
    58561 ttggctcgat gattatgggg ccgctcggtg gtctggtgat taaatatatc gacaagtcgc
    58621 tggaaaaacg catacctgct ggctttgaga tggttatcaa taacttctca ttaggtatcg
    58681 cggggatgct cctttgtctg ctgggttttg aagttatcgg cccggcggtg ttaattgcta
    58741 atactttcgt caaagagtgt attgaggcgc tggtacatgc gggttatcta cctttgctgt
    58801 cagtcatcaa tgaaccggcg aaagtgcttt tccttaataa tgcgatcgat cagggcgtct
    58861 attatccgct gggaatgcaa caggcttcgg ttaacggtaa atccatcttc ttcatggtgg
    58921 cctccaatcc agctcctggt ctggggctgc tgctggcgtt taccttgttt ggtaaaggga
    58981 tgagtaaacg ttctgcgccc ggggcgatga ttattcactt cctcggtggg atccacgaac
    59041 tgtatttccc gtatgtgctg atgaagccgc tgaccattat tgccatgatt gcgggcggta
    59101 tgtctggcac ctggatgttt aacttactgg acggtggtct ggtggctggc ccaagtccgg
    59161 ggtctatctt tgcttacctg gcactgacgc cgaaaggctc gttcctggcg acaattgccg
    59221 gtgttacggt aggtaccctg gtgtcctttg ctattacttc gctgatactg aagatggaaa
    59281 aaacggtgga aacgaagagc gaagatgagt ttgctcagtc agccaatgcg gttaaggcga
    59341 tgaaacaaga aggtgcattc tcgttaagcc gtgttaagcg tattgccttt gtttgcgatg
    59401 cagggatggg ctccagtgcg atgggcgcaa ccaccttccg taaacgcctg gaaaaagcgg
    59461 ggctggcaat agaagtaaaa cattacgcca tagaaaacgt
//
LOCUS       DAZFFE010000015        58908 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFFE010000015 DAZFFE010000000
VERSION     DAZFFE010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.015"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            12..179
                     /gene="yhfL"
                     /locus_tag="ACHJ69_001137"
     CDS             12..179
                     /gene="yhfL"
                     /locus_tag="ACHJ69_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO5014176.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            complement(462..1610)
                     /locus_tag="ACHJ69_001138"
                     /pseudo
     CDS             complement(462..1610)
                     /locus_tag="ACHJ69_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
     gene            complement(1622..1975)
                     /gene="yhfU"
                     /locus_tag="ACHJ69_001139"
     CDS             complement(1622..1975)
                     /gene="yhfU"
                     /locus_tag="ACHJ69_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO5014177.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(1986..2864)
                     /gene="php"
                     /locus_tag="ACHJ69_001140"
     CDS             complement(1986..2864)
                     /gene="php"
                     /locus_tag="ACHJ69_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO5014178.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYGFICQEMNDLM
                     ARGVRNVIEMTNRYMGRNAQFMLDVIRETGINVVACTGYYQDAFFPEHVATRSVEQLA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGCPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDFLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(2861..4087)
                     /gene="yhfW"
                     /locus_tag="ACHJ69_001141"
     CDS             complement(2861..4087)
                     /gene="yhfW"
                     /locus_tag="ACHJ69_001141"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO5014179.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLETLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKMGRIVREQVQVGRVITFGGLLPDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLAATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(4088..5251)
                     /gene="yhfX"
                     /locus_tag="ACHJ69_001142"
     CDS             complement(4088..5251)
                     /gene="yhfX"
                     /locus_tag="ACHJ69_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO5014180.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRACLPVAHQ
                     GHLVQIPCHQVVDAVAQGTDVITVFTLDKAREVSAAAVEAGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIQNLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGVTHTEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQRITETYLNAVDDSSIDYTL
                     PLAGEHPVSSAVVLCFRTQIFITRSDVVLVSGIHRGEPEIVGRYDSLGNPLEA"
     gene            complement(5335..5697)
                     /gene="yhfY"
                     /locus_tag="ACHJ69_001143"
     CDS             complement(5335..5697)
                     /gene="yhfY"
                     /locus_tag="ACHJ69_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO5014181.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(5714..6619)
                     /gene="yhfZ"
                     /locus_tag="ACHJ69_001144"
     CDS             complement(5714..6619)
                     /gene="yhfZ"
                     /locus_tag="ACHJ69_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO5014182.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLTHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVILTRVDDYPMQQLL
                     HAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(6779..7783)
                     /gene="trpS"
                     /locus_tag="ACHJ69_001145"
     CDS             complement(6779..7783)
                     /gene="trpS"
                     /locus_tag="ACHJ69_001145"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO5014183.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(7776..8534)
                     /gene="gph"
                     /locus_tag="ACHJ69_001146"
     CDS             complement(7776..8534)
                     /gene="gph"
                     /locus_tag="ACHJ69_001146"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO5014184.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADILMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSLNDLLPALGLPHSENQESKND"
     gene            complement(8527..9204)
                     /gene="rpe"
                     /locus_tag="ACHJ69_001147"
     CDS             complement(8527..9204)
                     /gene="rpe"
                     /locus_tag="ACHJ69_001147"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO5014185.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(9222..10058)
                     /gene="dam"
                     /locus_tag="ACHJ69_001148"
     CDS             complement(9222..10058)
                     /gene="dam"
                     /locus_tag="ACHJ69_001148"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO5014186.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(10165..11451)
                     /gene="damX"
                     /locus_tag="ACHJ69_001149"
     CDS             complement(10165..11451)
                     /gene="damX"
                     /locus_tag="ACHJ69_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO5014187.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKARNEEPEIEEELDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQIPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(11543..12631)
                     /gene="aroB"
                     /locus_tag="ACHJ69_001150"
     CDS             complement(11543..12631)
                     /gene="aroB"
                     /locus_tag="ACHJ69_001150"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO5014188.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLNTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(12688..13209)
                     /gene="aroK"
                     /locus_tag="ACHJ69_001151"
     CDS             complement(12688..13209)
                     /gene="aroK"
                     /locus_tag="ACHJ69_001151"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO5014189.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(13610..14848)
                     /gene="hofQ"
                     /locus_tag="ACHJ69_001152"
     CDS             complement(13610..14848)
                     /gene="hofQ"
                     /locus_tag="ACHJ69_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO5014190.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQTRAQANLPLENRNITLQYADARELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQGGGVGQVTT
                     LGSDLSVATATTHIGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(14760..15164)
                     /gene="hofP"
                     /locus_tag="ACHJ69_001153"
     CDS             complement(14760..15164)
                     /gene="hofP"
                     /locus_tag="ACHJ69_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO5014191.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPHWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(15154..15594)
                     /gene="hofO"
                     /locus_tag="ACHJ69_001154"
     CDS             complement(15154..15594)
                     /gene="hofO"
                     /locus_tag="ACHJ69_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO5014192.1"
                     /translation="MNMFFDWWFATSLRLRQFCWAVWLLVLVTLIFLSLTHHEESDAL
                     IRLRANHHQQWAALYRLVDTTSFSEEKTLPFSPLDFQLSGAQLVYWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFLLSVEGDDLLFTLQLETPHEG"
     gene            complement(15578..16117)
                     /gene="hofN"
                     /locus_tag="ACHJ69_001155"
     CDS             complement(15578..16117)
                     /gene="hofN"
                     /locus_tag="ACHJ69_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO5014193.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVNAVLLQAEQQLARSLQITKPRLLERQQLREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSIAALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(16117..16896)
                     /gene="hofM"
                     /locus_tag="ACHJ69_001156"
     CDS             complement(16117..16896)
                     /gene="hofM"
                     /locus_tag="ACHJ69_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO5014194.1"
                     /translation="MAFKNWQIGLHLQQQEAVAVAIVRSAKECLLHRWWRLPLENDII
                     KDGRIVDVQRLANTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQMAWL
                     SGTMARELDMDPDSLRFDYSEDSLSLAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPES
                     VALCGEDGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKVY"
     gene            17016..19568
                     /gene="mrcA"
                     /locus_tag="ACHJ69_001157"
     CDS             17016..19568
                     /gene="mrcA"
                     /locus_tag="ACHJ69_001157"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO5014195.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVVLSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRHDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRGTGQLANGGNSREEYFIEGTQPTQLAVHEVGTTIIDNGEAQELF"
     gene            complement(19734..20294)
                     /gene="nudE"
                     /locus_tag="ACHJ69_001158"
     CDS             complement(19734..20294)
                     /gene="nudE"
                     /locus_tag="ACHJ69_001158"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO5014196.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     APDFNEARNVSALFLVREWLKGQGRV"
     gene            20614..22749
                     /gene="igaA"
                     /locus_tag="ACHJ69_001159"
     CDS             20614..22749
                     /gene="igaA"
                     /locus_tag="ACHJ69_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO5014197.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTSEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFMMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            22814..23482
                     /gene="yrfG"
                     /locus_tag="ACHJ69_001160"
     CDS             22814..23482
                     /gene="yrfG"
                     /locus_tag="ACHJ69_001160"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO5014198.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            23493..23894
                     /gene="hslR"
                     /locus_tag="ACHJ69_001161"
     CDS             23493..23894
                     /gene="hslR"
                     /locus_tag="ACHJ69_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO5014199.1"
                     /translation="MKEKPAVKVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            23919..24797
                     /gene="hslO"
                     /locus_tag="ACHJ69_001162"
     CDS             23919..24797
                     /gene="hslO"
                     /locus_tag="ACHJ69_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO5014200.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(24860..26584)
                     /gene="yhgE"
                     /locus_tag="ACHJ69_001163"
     CDS             complement(24860..26584)
                     /gene="yhgE"
                     /locus_tag="ACHJ69_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO5014201.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNLSWIVYGV
                     PATVAFSSVLLFSVISFKQNRLWGWLALVFIATLGMSDWLKWQTDGMTPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNLLILLVIFLANGLTWLVLL
                     LWGELFKLVGITFFKTLFFATDGFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTADRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLDLQGKVNLVVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LADNVLIAPGSGKPDATFWSALIQDRYNVMTCIGKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSTRKEWDALDMSLLPRKITKEKLLTAAKDGKLGTRPKAWRDLVVDGE
                     RLDVNLNE"
     gene            26963..28585
                     /gene="pckA"
                     /locus_tag="ACHJ69_001164"
     CDS             26963..28585
                     /gene="pckA"
                     /locus_tag="ACHJ69_001164"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO5014202.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIIMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            28695..29012
                     /locus_tag="ACHJ69_001165"
                     /pseudo
     CDS             28695..29012
                     /locus_tag="ACHJ69_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493756.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            29071..29367
                     /gene="nadS"
                     /locus_tag="ACHJ69_001166"
     CDS             29071..29367
                     /gene="nadS"
                     /locus_tag="ACHJ69_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO5014203.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEETPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSRETKRRQPTGAPRKLLLLLQANPFIINQLKTI"
     gene            complement(29398..30750)
                     /gene="envZ"
                     /locus_tag="ACHJ69_001167"
     CDS             complement(29398..30750)
                     /gene="envZ"
                     /locus_tag="ACHJ69_001167"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO5014204.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGMTKEG
                     "
     gene            complement(30747..31466)
                     /gene="ompR"
                     /locus_tag="ACHJ69_001168"
     CDS             complement(30747..31466)
                     /gene="ompR"
                     /locus_tag="ACHJ69_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO5014205.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            31694..32170
                     /gene="greB"
                     /locus_tag="ACHJ69_001169"
     CDS             31694..32170
                     /gene="greB"
                     /locus_tag="ACHJ69_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO5014206.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            32267..34588
                     /gene="yhgF"
                     /locus_tag="ACHJ69_001170"
     CDS             32267..34588
                     /gene="yhgF"
                     /locus_tag="ACHJ69_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO5014207.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            35026..35253
                     /gene="feoA"
                     /locus_tag="ACHJ69_001171"
     CDS             35026..35253
                     /gene="feoA"
                     /locus_tag="ACHJ69_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO5014208.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            35270..37591
                     /gene="feoB"
                     /locus_tag="ACHJ69_001172"
     CDS             35270..37591
                     /gene="feoB"
                     /locus_tag="ACHJ69_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO5014209.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGSRQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLVIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDVELLATRKSVSSCCAASTTGDCH"
     gene            37602..37838
                     /gene="feoC"
                     /locus_tag="ACHJ69_001173"
     CDS             37602..37838
                     /gene="feoC"
                     /locus_tag="ACHJ69_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO5014210.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLKQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLHEWWALR"
     gene            38071..38664
                     /locus_tag="ACHJ69_001174"
                     /pseudo
     CDS             38071..38664
                     /locus_tag="ACHJ69_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            complement(38693..39463)
                     /gene="bioH"
                     /locus_tag="ACHJ69_001175"
     CDS             complement(38693..39463)
                     /gene="bioH"
                     /locus_tag="ACHJ69_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO5014211.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEVVLRQAPDKAIWLGWSLGGLVASQIALTHPERVLAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPDEFCHLLVALKQRV"
     gene            39501..40184
                     /gene="gntX"
                     /locus_tag="ACHJ69_001176"
     CDS             39501..40184
                     /gene="gntX"
                     /locus_tag="ACHJ69_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO5014212.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPLPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLNARLRKHNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWGLCRTL"
     gene            40243..40818
                     /gene="nfuA"
                     /locus_tag="ACHJ69_001177"
     CDS             40243..40818
                     /gene="nfuA"
                     /locus_tag="ACHJ69_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO5014213.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            41182..42498
                     /gene="gntT"
                     /locus_tag="ACHJ69_001178"
     CDS             41182..42498
                     /gene="gntT"
                     /locus_tag="ACHJ69_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO5014214.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(42609..44693)
                     /gene="malQ"
                     /locus_tag="ACHJ69_001179"
     CDS             complement(42609..44693)
                     /gene="malQ"
                     /locus_tag="ACHJ69_001179"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO5014215.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGKQYKGHVMGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESSSPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWKLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMADPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(44703..47096)
                     /gene="malP"
                     /locus_tag="ACHJ69_001180"
     CDS             complement(44703..47096)
                     /gene="malP"
                     /locus_tag="ACHJ69_001180"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO5014216.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRGNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQSDRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            47719..50424
                     /gene="malT"
                     /locus_tag="ACHJ69_001181"
     CDS             47719..50424
                     /gene="malT"
                     /locus_tag="ACHJ69_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO5014217.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICDTMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGSPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTSDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFESAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(50467..51483)
                     /gene="rtcA"
                     /locus_tag="ACHJ69_001182"
     CDS             complement(50467..51483)
                     /gene="rtcA"
                     /locus_tag="ACHJ69_001182"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO5014218.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGVHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNSLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLQTNIAVVERFLPVRFSLIETDGVTR
                     VSIE"
     gene            complement(51487..52713)
                     /gene="rtcB"
                     /locus_tag="ACHJ69_001183"
     CDS             complement(51487..52713)
                     /gene="rtcB"
                     /locus_tag="ACHJ69_001183"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO5014219.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSVTQKTVKQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            52902..54499
                     /gene="rtcR"
                     /locus_tag="ACHJ69_001184"
                     /pseudo
     CDS             52902..54499
                     /gene="rtcR"
                     /locus_tag="ACHJ69_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(54481..55239)
                     /locus_tag="ACHJ69_001185"
     CDS             complement(54481..55239)
                     /locus_tag="ACHJ69_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO5014220.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTMPPASVMQVLKDHHIQLELC"
     gene            complement(55256..56086)
                     /gene="glpG"
                     /locus_tag="ACHJ69_001186"
     CDS             complement(55256..56086)
                     /gene="glpG"
                     /locus_tag="ACHJ69_001186"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO5014221.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLGNPADPRYLAASWQSGHTDSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(56131..56457)
                     /gene="glpE"
                     /locus_tag="ACHJ69_001187"
     CDS             complement(56131..56457)
                     /gene="glpE"
                     /locus_tag="ACHJ69_001187"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO5014222.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAAQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            56647..58152
                     /gene="glpD"
                     /locus_tag="ACHJ69_001188"
     CDS             56647..58152
                     /gene="glpD"
                     /locus_tag="ACHJ69_001188"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO5014223.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIMRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARREKGLWIVEAEDIDTGKKYTWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLNRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDEDGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGENFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLMEYTQQRLSLAS"
     gene            complement(58206..58811)
                     /locus_tag="ACHJ69_001189"
     CDS             complement(58206..58811)
                     /locus_tag="ACHJ69_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014224.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIALAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELLVEYALFFSVMGGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCQWRSKNRSHRKKWGDWVTCLSLAILALGPLFNYLQ"
BASE COUNT        14231 a        16042 c        14757 g        13878 t
ORIGIN      
        1 agggaatgta aatgaacaaa tttattaaag ttgcactggt aggcgcagta ctggctacgt
       61 taactgcatg tactggtcat attgaaaacc gtgataagaa ctgctcttac gactacctgc
      121 tgcacccggc tatttctatt tctaaaatca ttggcggttg cggtccgact gcacagtaag
      181 tctctgacag aaatcggcta acgccggttt aattgcctga cgccctcgga gttatccgag
      241 ggcgtatttg tttctgcatt cttttcaaaa atatcgttta caacgattaa atgctggcaa
      301 tactttcacg caagatccgc aatatcgtct cttcaccgct gcgatttggg ttaatgcgaa
      361 tcgcgcaatg ttctaactgc ggattcgcct ggcgaaacgt tccggaaagt cgataaaaga
      421 gcggcgggat ttcatatttt gactctgcac caaccgggta aggcaatttt gattgctgca
      481 aagatcgaac cgaccagcaa cgccacttcc atcagcatat tcatggcgtt acggatgtta
      541 tccgacgcat tacgtaccga cgggtagcgc cccaaccatt tgccgatcga acgaagcagc
      601 aagacttccg ccgaaataac cactgcgcct aataccgctg caaccatcgg attcggcgag
      661 agatagccca ccgcataaac aaaggtaaag cccgcaactg catacacacc ggtggctaac
      721 gcggtggtgg caatcaacgg cacaaaaccc agcccgcgca taaattctgc cagtgccgcc
      781 tggttgatta gtgtttgcga ttgttccggc gttacgcctg cggagtacgc tttctccagt
      841 gtgaagatcg acacttcact accggcgaaa atcttcatgc tggcaacggc agcaatcaat
      901 gcacccacga tggcgatata cggtaagttt ttgataatcc gtgatgtacg ctcttcaaac
      961 accgacatac cgctggcatc gatatcattt tcatcacgat gacgcaggtc atgagttatt
     1021 gcgatcccca gcagcatcac catgccaata aagatttcga tggattcagg gttaagatgt
     1081 ggggaatagc gcactaccac cacacgagtc atcagtacca ccacggcggc gatcagactt
     1141 tgcttccagc caaactggta gaaaatcgcc accaacggga acagtgcaaa agctgaaacc
     1201 accggcgagc ttaattcccc caggctacct aatacatcca ccggtagcgc ggtcagcagc
     1261 tggtttactg gcaacagaca ggtgaggatc aatattcccc agatagcacc aagaccaaac
     1321 gccatcaggc tgtttatcgc cagtacgccg aggatatcgg taggaagaaa gagtaaccat
     1381 gcgttgagca gcccggtttt cagggtaaaa gagatcccca ccgaggccac aaaaccaatg
     1441 ctcagaccaa aagcgatact ccccgcctcg cgacggttca tatagccttc aatcagttgt
     1501 ggcaggatcg ggcggatacc gtcatgaaaa acggccgccg agcgatgcgc cagaagcgat
     1561 gtcatacccg tcaggcacgc caccacgata atctgaatat acagatccat agtcgtgcct
     1621 tttatttcag atggttaatc agcatcggaa tcgcgtgttc aacgtgctca acggaaaggc
     1681 caaacgccac ttttccttca gcgatcattt tggcgatatg ttcgtctttc gctttaatgc
     1741 ccggcttggc aatggtgcaa cttttgttat agccaatcac cgcgatagca atcgacaatg
     1801 ccgcgcccgc accggtatta cacgcaccga tgtagtaatc cagttgcccg gatttcactt
     1861 tcattgccgc ttccatgtcg ttgtgaatga aaacttcaaa acagccagga gccgtcgctt
     1921 caatggtttt tttaatctgc tcacgctgta agcctgcaac gccaatcttt ttcatgagtc
     1981 tgtccttatt ggaaaaattg agagggattt tcacgtaaca tcacatccac atcggcctga
     2041 ctgaatcctg actggcgcaa ttgcggaata aaggtggtca ataaaaagtc atagccataa
     2101 ccaccgttgg cttttaaatg agagcgccgc gtaatatcca tcgacagcat gacgcggttc
     2161 agcaacccac ggtcacgtag cgcatggagc atcgcaatgc gcttttcgtc cgggtagtaa
     2221 ctgttcttgc cgatggtgtc gaactgcaca tacgcgccga gatcgatcat cttcaaaatg
     2281 ttgtcgagat tgtctttcag atcgcagtgg ccgacggtga cgcgcgaaag atcaactccg
     2341 tgggcttgca gtagcgccag ttgttccagc cccatggtgc taaaagaggt atgtgtggag
     2401 atcgggcatc cggtctggtt atgcgccaac gcggcggcaa taaacacctt ttcttccagc
     2461 ggcgtaatct tcccttcgct ggagccaatc tccgcgatga tcccggcttt cagctccgtg
     2521 ccatcgatac cctgttcaat ttcatcgacc atctcctgcg ccagttgctc tacgctgcgg
     2581 gtcgccacat gttccgggaa aaacgcgtcc tggtaataac cggtacaggc caccacgttg
     2641 atcccggttt cgcgtattac atcgagcata aattgcgcat tgcgccccat ataacggttg
     2701 gtcatctcaa tcacattacg cacgccccgg gccatcaggt cgttcatctc ctggcaaatg
     2761 aacccatatt gatcaaggcg gcagtccacg ttgtttttaa agccggagag atcaatatgc
     2821 agatgctcat gggcgagcgt gtaacccgtc ggatcaaaac tcatagggtg tctcctgcaa
     2881 accggaggga ggaaagaaaa gagcgaccat tttgtggcgg tggcgcacgg aaaaattcac
     2941 acactgtagc cccaacatca gaaagcgtgg tgcgaacacc aagctgagta gcagccaggc
     3001 cttgctgata aaccagcacc ggcaccactt cgcgggtatg gtggctgtga ccaatggtcg
     3061 gatcgttacc gtgatccgcc atcacgacca ggcaatcatc tggctgcatc gcctcaacaa
     3121 gcctggcaag gttacggtca acgacctgca aacgttcggc ataacgtgcg acgtcttctg
     3181 catgaccagc gaggtcggtt tcctgaatgt tggtgcaaat aaacgctgtc ggatgcgtgt
     3241 taaattcgtc gagggtgata tccataatcc tctggctatc caccaggttt tgccagctca
     3301 cgccataagg attgctgacg atatctgcca ccttacccac cagcacggtt ggcacgcccg
     3361 cttcatacag tttttgcggc acctgcactt tttcatcaac accatagccc atatgcacga
     3421 cctggaaacc gttgtcataa gcgccagaac gcggcgcgtt gatgccaata aagcgccctt
     3481 ctttgctttc tgcggcatcg agaatgcgtt gactgtcggg taacaggccg ccaaatgtaa
     3541 tgacccgacc gacctgtacc tgctcacgca caatacgacc cattttgatt gcgtcgtcaa
     3601 aagagatcac agagagattg gcggtgatgt tatagacctg gcctaaatcc gcctcgagat
     3661 tatcgccaat cgcaaccgcc tgattgaccc acagaaattg cagatcatcg ccacgacgct
     3721 ccacctgcca accggcggat actaatgcct gctcaacacg gtcaatcaca tcacgaaagg
     3781 gcatccgcag cggcggtaat gggcgcgtac ctaaaatctc ctgatgcccc ataaaggtat
     3841 cgccaccttc atgttgcagc tctgccacgc cccaggttgc ggagtctgac ggctgcatat
     3901 cgccaggcgc ataacccaat gcgttgatta gccccagcgt ctccagcgtt ggtagctgca
     3961 aatgcggcaa ctggctcagg atgtgaccac aggtattcgc cccggcatcc tgcggacgca
     4021 ccaacgtgac atctttcatt gcccctacgc caaagctatc aatcactaac accacaaatc
     4081 gcgccattta cgcctccaga gggttgccga ggctgtcata acgaccaact atttctggtt
     4141 cgccacggtg aataccggac accagcacca catcactacg ggtgataaaa atctgcgtgc
     4201 gaaaacagag caccactgca ctgcttaccg gatgctcgcc tgccagcggc agggtgtagt
     4261 cgatactgct gtcatcaacg gcgttgagat atgtttcagt aattctttga ttttctggcg
     4321 taaacaccag cgcatgttgc gcgtgaccac gacggtagta accgccgccg tagcagtagc
     4381 tgtcgccacg gaaatgatgg gagatttcac ttaaccagag catcgcgata cgttcaggct
     4441 gatcgccctg ttgatttgcc ggaatagtac ccgtcagcgc atgaccgggt tcggtatgag
     4501 tcacaccgta ttccgccagc aatggcagcg acgtgcagct ggtcgctgaa ggcgcgttca
     4561 gttgctcaat cgcaatacca gatttcgcca gttgatcccg tgcttgtacc agcgtgtgaa
     4621 gattcggtgt cggcaaaact ttcccggcag cctcatccca aagcaggcaa gggaaatggg
     4681 taagtcccgc caaatgtagc cccggcagat tctggatttc agcaaccacc tcgtgcagcg
     4741 aatgctgtac aaaaccgctc tcctgccccg gataaagaaa atcatcgtcg ctataaactt
     4801 tgagcagcac agactgcaca cgtccggcct ccaccgctgc cgcagaaact tcccgcgctt
     4861 tgtcgagagt aaacacggtg atgacgtcgg ttccctgtgc aacggcgtca accacctgat
     4921 gacaagggat ttgtaccaga tgcccctgat gcgctaccgg caaacaggcg cggcgcatga
     4981 ctcgcgcctc tttgtaatcc accgccacaa tgccgctgta gcccagagcc aacagttttt
     5041 ctgccagcca cggattacga ccgaattgtt tggtcatcag atacagttca atgccgtaaa
     5101 gccgcgccgt ctcaatcagc cgcttgccgt tttccagaac ttgatccacg tcgatcaccc
     5161 aactgtccgg ggcgatcttg ccctgctgcc acaaacttag tgcggcggaa atcagcgccg
     5221 ggttctggcg tttcaatgct tctacaaaca tcttgttgcc tcgtttctgt tccttgaatt
     5281 aaatattcat tttttcgaat atttaggttg tgcgacgtgg atttatcggg agcttcaaac
     5341 ctcttcgtta gcagccatcc ataatccata aaggttggcc agcaaatagc cttcttcaca
     5401 cgggttaact tccagtgcaa attccttcaa caacacctga tgcaattgca caacggcttg
     5461 ccagtggctg gattgcgcca gttccgccag caattcgtca tccagcggct ctatttcttc
     5521 accacggcga ctgcgcatca gtgcactcgc catatgtgtc atcgccatcg ttccttgctc
     5581 actgcgcacc ggtagatggc actctgtttc cagtacattg acgacctgca tcatgccttt
     5641 gcaaatgtcc ttatcaataa cgcctgcatc gcaaagcagg ttgagtctgg tttccatatc
     5701 agatgccttt cgtttaataa ctgggttcct gttccccact gactactcgc tgttgatgag
     5761 caagcagggc atgtttatca acaactgcat gcagcagttg ttgcattggg taatcatcga
     5821 ctcgcgtcag gatcacggct tcggtggcct gtaaaaatcg cggatcgtct gtgagcggcg
     5881 tcgcttctaa tcccagcatg gtcagttcgt tttccgccac tacgttccag ataaccgcgt
     5941 caacatcgcc tttaacaatg cgttgtaaac tctcgtgata agagagatcg actcgttcca
     6001 catcactatc gccaaaaaag acatcggtca tgattttctg atctgccgaa cggttatcca
     6061 gccccacgcg cttcacgttt gcggactcgc ctttacggca aatcaactgg tgctcgccaa
     6121 cgtaggtatg cggtcccaac tccagcgcga ggcataatcc tttttgcgtg agataacttt
     6181 ccgccgccag tcgcgaaacc accgccatgt catacacgcc gttaagtaaa cactccacgc
     6241 gaatatccgc gccgcgcata tgcgcatagt aaaaaggaat gccatcaaac tgggctttca
     6301 atccgctcgc caggccttcg tacaaacggg tatagggcaa gggcatcgca cataccacgt
     6361 tgttgatatc cacatgagtc agcaatgctt tgttatccat ctcaaccaga taactgccat
     6421 tgcgcccacg gcgttcaatc cgtatcgctc cgcttgattc cagcgttttc aacgctgcct
     6481 gcgtcaggcc aaccgatgaa cggcattcag ttgccagctc atctatggtt ttcagtcgat
     6541 taccgcactt ttcacccaac aaataacggg ccagtgtcgt gataacgacg ccttcttttt
     6601 tgataaacgt tcgacgcata atatattttc aataaaatga atgtttatat cttcaggaat
     6661 ttgaagataa ggcgcaacag cgagatgtgg aaacggcgag gcgcttcaca ttttttcgga
     6721 ttattgcaaa tgaggataaa aaaaccgggt ttccccggtt tttggcatta atgttaaatt
     6781 acggcttcgc cacaaaacca atcgcttcgt acaccgcttt tagcgtacgg gaagcgtgtg
     6841 cgctggcttt ttctgcgccg tctttcatca cctgttgcag gaaggcttca tcgttgcgga
     6901 aacggtggta acgttcctgc aattcagtca gcataccgga aacggcatca gccacttcac
     6961 ctttcagatg accatacatc ttgccttcga actgtttttc cagttccggg atgctctggc
     7021 ccgttaccgc agaaaggata tccagcaggt tggaaacgcc cgctttgttc tgcacatcgt
     7081 agcgaactac cggcggctcg tcggagtcag tgaccgcgcg tttgattttc ttcactaccg
     7141 atttcggatc ttccagcagg ccgataacgt tattgcggtt atcgtcagac ttggacattt
     7201 tcttggtcgg ctccagcagc gacattacgc gcgcgccaga tttcggaata aacggctccg
     7261 gcaccttaaa gatatcgccg tacaacgcgt tgaaacgctg ggcgatatcg cggctcagtt
     7321 ccaggtgctg tttctggtct tcacccaccg gcaccagatt agtttgatac agcaggatgt
     7381 ccgcagccat cagcaccgga tagtcaaaca gaccagcgtt gatgttctcg gcataacgcg
     7441 cagatttatc tttaaactgc gtcatgcggc tcagttcgcc gaagtaggta tagcagttca
     7501 gtgcccagcc taactgcgca tgttccggca cgtgggactg aacaaaaatg gtgcttttct
     7561 caggatcgat accacaagcc agatacaacg ccagcgtatc cagcgtcgct ttacgcagct
     7621 tctgcgcatc ctggcgcacg gtgatcgcgt gttggtcaac gatacagtaa atgcaatggt
     7681 agtcatcctg catgtttacc cactgacgca gcgcacccat gtagttacca atggtcaatt
     7741 cacctgaggg ctgtgcgcca ctaaaaacga tgggcttagt catttttcga ttcctgattt
     7801 tcgctatgcg gaagccctaa tgcgggcaga aggtcattta gagactgata aattacatca
     7861 ggctggctga gatcgatagc ctcgccgtag ttatatccgt aggttaagcc aactgatgga
     7921 caacctgccg cttttgccgc ctgaatatca ttgcgtgagt cgccgacaaa cagcatctgt
     7981 tgtggagcaa tgcccatccg ctcagccacc agtagcagcg ggtccgggtg cggtttttta
     8041 ttttgcacat catcaccacc aatcaccacg ctgaagtatt tggcgatatc taaggcttcg
     8101 agcagcggcg cgacgaacgg cgtcggtttg ttggtgacca ggcctagcgg caggccttta
     8161 gcctgcaacg cgcccaacgt atcggcaacg tgcgggaaca aaaacgtccc ctcttcggca
     8221 acctcgccat agtagcgatc gaacagttta cgcaggatac gtacctgttc ttctgccgga
     8281 atgtcgtcat caacgggcgg tttacccatt gttttacgca gagtcgcacg ttcctgacgc
     8341 gcccaggtca atgcgcgctc catcagaata tctgcgccgt taccaatcca ggtaataacg
     8401 cgttcttcac ctgcgacagg caactccagc gcatacagcg ccatatctac cgcagcagca
     8461 agaccgggtg cactgtcaac cagcgtaccg tcgagatcaa aagcgacgcc gcgaatatct
     8521 tcaaacttat tcatgactta cctttgccag ttcactgcgc atttcatcaa tgactttttt
     8581 gtagtctggc tggtcgaaga ttgccgaacc agcaacaaac atatccgcgc ccgccgcagc
     8641 gatttcgcca atgttgttca ccttcacgcc accgtccact tccagtcgaa tgtcaaagcc
     8701 agactcgtcg atacgacgac gtacttcgcg cagtttatcc agtgtttgag gaatgaaaga
     8761 ctgaccgccg aaaccaggat tgacggacat cagcaggatc acatccagct tatccatcac
     8821 gtaatccaga tagctcagag gtgtcgccgg gttaaatacc aaacccgctt tacagccatt
     8881 ttctttaatc agttgcagcg tgcggtcaac atgctcggag gcttctggat gaaaggtaat
     8941 gatgctggca ccagcggcag cgaaatcagg cacaatgcga tcgacgggtt tcaccatcag
     9001 atgtacgtcg ataggggcgg taatgccgta gttacgcaag gatttcagca ccattggccc
     9061 aatcgtcaga ttgggaacat agtggttatc catgacgtca aaatgcacga catcagcgcc
     9121 agctgccagg gcttttgcgg tatcttcacc caggcgggca aaatcagccg acagaattga
     9181 gggggcaatc aaatactgtt tcatccgctt ctccttgaga attatttttt cgcgggtgaa
     9241 acgactcctg gtttgtacaa agccagcagt tcgtccacct ttttacgtgt gccgccgttg
     9301 ctgcttatac tgcgtcgaac tttgacgaca tgcaattttg cgcgctgata ccactcacgc
     9361 gttaacatcg tatcgtgatt ggagatcagc accggaatat ggcgatcaac cagaccttca
     9421 gcgatctccg ccagatgcgc ttgttgttca agcgtaaaac tgtttgtgtg atacgccgta
     9481 aagttggcag tcgcagacag cggcgcataa ggcggatcgc aatagacgac agatgcatca
     9541 tctgcgcgcg ccatgctatc ggcgtaagac tcacaataga aaaaggcatt ctgcgctttt
     9601 tcagcgaagt gatacaactc tgcttccggg aaatagggtt ttttgtagcg gccaaacggc
     9661 acgttaaact caccgcgcag attgtaacga cacaggccgt tgtaaccgta gcggttcaaa
     9721 tataaaaaca gtaccgcccg acggaacgga tcctggcttt tgttgaactc ttcgcggaac
     9781 tgatagtaaa cctcggcgca atttgtttcg ggaacaaaca gctcgcgtgc ggcctgtacg
     9841 tactcatcag tacgcatctt cacaatgtta tagagactga tcaggtcgct attgatatcg
     9901 gcaaggatat aacgagaaaa gtcggtgttg agaaacaccg acccggcacc tacaaaaggc
     9961 tcaaccagac attcgccctt gggcaaatgc cgtttaatat catcaagcag gggatacttg
    10021 ccccctgccc acttcaaaaa agcgcgattt ttcttcatgc tgactaacta attacacctt
    10081 ctccggctgt ggagaaagct ccgacagcat ccagcgcttc aggcaacgcc cgcaatgata
    10141 aaccattgcg ggagataacc ttgattactt cagatcggcc tgtacctgac gcagcggttt
    10201 cgcccacggg tttttggcct ggacatctgc tggcaatgta gataccgctt ttttcgcctc
    10261 ttctttcgaa gcatacacgc cagaaaccag gacataccac ggctgaccat tacgcgtcgt
    10321 ttcatagaca acgtagtttt tcagattctc tttcttcgcc caaccgttca ggttgtcgta
    10381 gttagaggaa ctgctcagct gcagagtgta atggctggac ggtgccgatt tcaacgaacc
    10441 aacattacct gcggtcttcc ctccagcggc tggtgttgcc gtggtttgcg ccggggatgc
    10501 cgtctgtact ggagccgtag tcgccgtctc ttttggtgct ggcgtagaag tcgcagccgg
    10561 tgcctttgtg ctcgccacag gggcagctgg ttcagttcgc ttcggcgtct gtgcgactgg
    10621 cttcggttcc gttttcacgg ttgcttgcgg ttttttcggt tcaatcaccg cctgctgacg
    10681 agccgggcgc gtagtggccg gacgttcagc ggtttgcgtt ttcgccgtgt cacgcgatgc
    10741 attgccattg cgaacaggcg caaccgttgc gggttcggtc ggcaatgtag aattgaccgc
    10801 cacattgttc agctgttgct gattttgtgg ctgggtcagc gcattgttca ggtcaccctg
    10861 cacttcaaca cgttgttgac catccgttgc cgccgggatt tgcccttgag tcggcgtaga
    10921 agagatcggc ggcagagaaa catcctgctg agtattttcc gcagacgtgg ttcccggcgc
    10981 tggctgcacg ccattggcct gatcggtcgc attgcctgca agatcaatac tcttctcgcc
    11041 agacgcggtt tgatcgctgg aagaggtcga gggggctttt agcgcagaac cgataccgat
    11101 gatcaacagc agtagaacca gaatgccgac acccatcatc atatactgac gagaagcggg
    11161 tttactggct gcttttttgc gcttacgcgg acgacgctct acgcgctctt catccacggt
    11221 ttcatcttcg gattcgtcaa gttcttcttc gatttccggc tcctcattac gcgctttttg
    11281 cgcacgagtc ggacggcgat cgtcagtgtc atcaagttca atatcatcaa aattgatctg
    11341 cggttcgcca cgttcagtac gctcagaacg ttcagaagat tgacgagaac gaccagtacg
    11401 acgatcgctg ggatcgggtt tcagctcgtc ttctggtttg aattcatcca tttaacaccc
    11461 cactaaaagg ttaatgctta ccacgttgca attaacctga agctaataga ccgcttgata
    11521 agcggcctga cctttcttgt tgttacgctg attgacaatc ggcaatggcg ttaagaacaa
    11581 gctcgtgcga aacgccgctg cgaacttcac tcttaccaat tgccaacgga agaattaagc
    11641 gcatctctcc cgcaaggact ttcttgtcac gcagcatatg cggtaaatac gcctgcgcgg
    11701 acatttcgcg cggcccattg accggtaacc cagcccgtgt gagcagggtt ataatacgct
    11761 gcgtttcggc agaactaaac tgcccgagac gttccgacgt ccgcgccgcc atcaccatac
    11821 ccgcagcgac cgcttcacca tgtaaccaat tgccataccc catttcagct tcaatggcat
    11881 gaccaaaggt gtgtcccaga ttcagtaaag cacgtaaccc ggtttcgcgc tcgtcggcgg
    11941 cgacaacttc tgctttcagc tcacaacaac ggcgaataca gtacgccatt gccggaccgt
    12001 ccagacgcaa caacgcatcc agattctctt ccagccagtt aaaaaacgca ccgtcaagaa
    12061 taatgccgta tttgatgact tctgccagcc ccgacgctaa ctcacgcggg ggaagcgttt
    12121 tcagacagtc gagatccacc accactgaag cgggctggta gaacgcgcca atcatgtttt
    12181 taccgagggg atggttgacc gcagttttgc cgccaacgga ggagtcgacc tgagacagta
    12241 acgtcgtcgg gacttgaatg aaacgtacac cgcgctgata actcgccgcc gcgaagccgg
    12301 tcagatcacc cactacgccg ccgccaagcg ccaccagagt agtatcgcga ccatgcggct
    12361 tttgtaacaa tgccgtaaag acggtattga gtacagccag gcttttatac tgctcgccgt
    12421 cagggaggat aacgctatcg acgttaacac ccgcctgttc aagtacgccg cggaccttat
    12481 cgagatacag aggagccagg gtttcgttgg tgaccaacat gacctgctcg cccgatttca
    12541 gcggtaagaa tgaagctggt tcattaaaca aaccagatgc gatggtaatt gggtaactac
    12601 gttccccgag agtaacgaca atcctctcca taacgcgaca tccaccttaa ttactgtacc
    12661 cgcagacgag tgtatataaa gccagaatta gttgctttcc agcatgtgaa taatctggtt
    12721 tgcaaccact ttagcgcttt gatcatcagt acgaatggtc acgtcggcaa tctcttcata
    12781 cagcggattg cgttcattgg ccaacgcttc cagaacttca cgcggcggtg tttcaacgtg
    12841 cagcaacggg cgttttttat cacgctgcgt gcgtgcaagt tgcttttcga tggtcgtttc
    12901 aagataaacg acaacgccac gagcggaaag acggttacgc gtttcacggg atttcacaga
    12961 gccgccgcca gtagccagca caataccctg tttctcggtc aactcattga tgaccttttc
    13021 ttcgcgatcg cggaagcctt cttcgccttc taaatcgaaa acccagccca catcagctcc
    13081 ggttcgtttc tcaatctctt gatcggaatc gtaaaattcc atattgagtt gttgagctaa
    13141 ctggcgccca atagtgcttt ttccggcacc cataggccca accagaaaga tattgcgttt
    13201 ctctgccatt ttttcggtac tactaagact attcgttaat gataaacccg cttcgctcag
    13261 agagcgccgc aggacatgaa ctgaaacctc ataagatatt gcgagagtca gactgaaaat
    13321 tatctcaata ctccagcggg tttggcaact gaataaatca cccatccctc tgcatatctg
    13381 gtcgctgcaa gcgcgctgcc ttgctaccac cgctctggcg ataaatcacc gggtaagatt
    13441 agcgtaaaaa agacagcaaa atgccgcctg aatgataaat catcatcatg gaacgcaggc
    13501 agcgaagagc accaactctc aaatcggtac tccttgtatg ctaaatacct gcgcgcgtca
    13561 aatagatgaa acacgttcag cgtaaaaaca ttaccgcttt tacggctgtt tactcactgg
    13621 aaaccagtcg tggcgtgata aacaccacta actcgcgtcg ttcatcttct tttccgtcat
    13681 gacgaaataa ttgcccgaac cagggaatgt cgccaagcaa cggtacgcta tcctgacccg
    13741 atttattttt acgggtaaaa atgccaccca gcgccaacgt ttctccgctt ttgacctcga
    13801 cctgcgtttc gatctcctgc ttatcaatcg ccagcacttc gccatctgcc tgctgtagca
    13861 cctgccccgg aacgttctgg ctgatgtgta atttcagccg gatgcgacct ttttgtaaca
    13921 ccgtgggcgt gacttccatc cccaggacgg cctctttaaa ttctaccgac gtcgcgccac
    13981 tttccccgct ggaaacctga tatggaattt cgctcccttg tttaatgctg gcaggctgaa
    14041 gatgtgaggc cagcagacgc ggactggcga taatatccag ctgctgtttt tgttcgagcg
    14101 cggaaagctc aagatccagt aaacgtccgt tgatgcgtcc aatgttaaaa ccgatatgcg
    14161 ttgtcgccgt cgctacggag aggtcgctgc caagcgtagt gacttgccca acgccaccac
    14221 cttgttgcgc atcggccaac gtccatttca cacctaactc acgcaaactt ttttcattaa
    14281 tggtgacaat atgcgccgac agctcaacct gcccgaccgg cagatccatt tgcgataccc
    14341 actgttcaag cgtgcttaac gccgttttgt tatctcgcag caaaaggcga ttggtgcgtt
    14401 tatcgacggt catactccct ttggcactca gtagcttctc ccccgctttc gccagctctc
    14461 tggcgtcggc gtattgcaga gtaatattgc gattttccag cggcagattt gcctgcgccc
    14521 gcgtctgctc cgcctcctgg cgggcgatat tgtcattctg ccaggcaacg gaatgcaccg
    14581 agagaatgtt gccctcctgg cgcgttatca atccggcgct tttcactaca gtttgtagtg
    14641 cctgcttcca gggaacgtca gttaaatgta acgacaccgt accgctgacg tctggcgaaa
    14701 ccaccaggtt caacttctcc tgttcagcca gcgcctgcaa cacctgagct accggaacgt
    14761 catccaccat cagcgtcact ttttgcggct ttgccgcctg tacgccgggt atcagcatca
    14821 acagtagtgc ggctatccat tgcttcattt gtatctcctt gtcgttgcca caaccagtgt
    14881 ggcggttcgc agtttgtccc ggttcccagc gttagtgcgt ctggcgtcag ctgtaagatc
    14941 gtccagccgt tttccagcac atcgttttgc tgcacccgtc gccatttttt ttgcccatct
    15001 tttattacac cgatgatgcg ctcgcctcgc cctaccatcc cctgatagcg ccactggcta
    15061 agttcgctaa tccggcatag atcttcgggc ggtttaaaag ggtcacgcat accggttaaa
    15121 aggcacaatg caatgcctgc taacaaccag cgtttaaccc tcatgcggcg tctccagttg
    15181 tagcgtgaac aaaaggtcat caccttccac gcttaacaaa aaacggctca cgctgacgtt
    15241 gcgctctgcc agccgtgtaa atgccgacgg cactgcttcc cacagcgttt tcaacgccaa
    15301 ctcacctccc tgcgcggatg gatgccagta aaccagttgc gcgccggata actgaaaatc
    15361 cagtggcgaa aagggcagcg ttttttcttc gctgaaggaa gtggtgtcta ccaggcgata
    15421 cagtgcagcc cactgctggt gatgatttgc ccgtagtcga attaatgcat cgctctcttc
    15481 atggtgtgtc aatgacagaa aaatgagcgt aactaacacc agcaaccaga ctgcccagca
    15541 aaactggcgg aggcggagtg atgtggcgaa ccaccagtca aagaacatgt tcatcgctaa
    15601 ccttccttgt taactgatac tcaaattgcc agcgtccctg cgcatcctgc tgcgtggccc
    15661 ccctctgatt gagatgaaaa gaagcatcct ggcggagtga cgtttccagc gcgtttaacg
    15721 cggcaatgct tgttgtcagc cccttgatct ccagcgttcc ctgctgccag cttatcgttg
    15781 tcagccaggc atgttcgggt aaaagcgccg ccagtgcttc cagcgcagat tgccagtcgc
    15841 gggtaaattg tcgctggcgc tgcctttgca aacgctgttc gcgtaactgt tggcgttcca
    15901 gcaaacgtgg tttcgttatc tgtaagctgc gggcgagttg ttgttccgcc tgaagcaaaa
    15961 cggcgtttac gcgtgcttcg gcattgcttg tcagacgcag tattagcgtt atcccgacgg
    16021 ccagcagcag aggcgcaacg aacatcagca accagaaacg aagaaaagca gtccggcgtt
    16081 gctgtcgcca gggcaaaaaa ttaattggcg ggttcatcag tacaccttcc caagcgccag
    16141 ccccagcgcg atggcaaagt ctccacctgg tggcggtagc ggcggctgac gaacagaaac
    16201 cgcctcccag gggtcaaatc cgtcttcgcc acatagcgcg acgctttcgg gatcaacaga
    16261 taacgctgcc gccagctcct tcgcgctagt catccccacc gccagtttgc gcccccagct
    16321 atagcgcgtc gcccacagcc actgttcgtt atcacgccag gccagacatt gctgatgaga
    16381 aggtaaaaaa ggcaggaatc gctgtaatgc actggcatcc ggagtgatcg cgctaacatg
    16441 aacacgcaac ctttctgcca gagtaagcag cgttgccagt tctttgcttt gcgcggcagt
    16501 cacgttatag gcgagactga gtgagtcttc gctgtaatcg aagcgcaggg agtccggatc
    16561 catatccagt tcacgggcca tcgtcccgga cagccaggcc atttgctccc gctcaccgag
    16621 ggacatcgac gggcgtggaa atgaccgctg taatgtgcga ctggcgggaa acgctaacat
    16681 aatgtgatga cgctgcggca gttcacgact ccacggtaac aacgtgttag ccagccgctg
    16741 cacatcaaca atccgcccat ctttgataat gtcgttctcc agtggcaacc gccaccagcg
    16801 gtgcaataag cattcttttg cgctccgcac gatagcaacc gctaccgctt cttgctgttg
    16861 taaatgcaaa ccaatttgcc agttcttaaa tgccattgtg atgatctcct tatcacccgt
    16921 cactctgaca ggtatatcaa tgcgtctggc ttgcctttat actaccgcgc gtttgtttat
    16981 aaactgccca aatgaaacta aatgggaaat ttccagtgaa gttcgtaaag tattttttga
    17041 tccttgcagt ctgttgcatt ctgctgggag caggctcgat ttatggccta taccgctaca
    17101 tcgagccaca actgccggat gtggcgacat taaaagatgt tcgcctgcaa attccgatgc
    17161 agatttacag cgccgatggc gagctgattg ctcaatacgg tgagaaacgt cgtattccgg
    17221 ttacgttgga tcaaatcccg ccggagatgg tgaaagcctt tatcgcgacg gaagatagcc
    17281 gcttctacga gcatcacggc gttgacccgg tggggatctt ccgtgcggca agcgtggcgc
    17341 tgttctccgg tcacgcgtca caaggggcga gtaccattac ccagcagctg gcgagaaact
    17401 tcttcctcag tccagaacgc acgctgatgc gcaagattaa ggaagtcttc ctcgcgattc
    17461 gcattgaaca gttgctgacg aaagacgaga tcctcgagct ctatctgaac aagatttacc
    17521 tcggttaccg cgcctatggt gtcggtgctg cggcacaagt ctatttcggt aaaacggtcg
    17581 accaactgac gttgaacgaa atggcggtga tagccgggct gccgaaagcg ccttccacct
    17641 tcaacccgct ctactcaatg gatcgtgccg tcgcgcggcg taacgtcgtg ctgtcgcgga
    17701 tgctggatga aggatatatc acccaacaac agttcgatca gacacgcact gaggcgatta
    17761 acgctaacta tcacgcgccg gagattgctt tctctgcgcc atacctgagc gaaatggtgc
    17821 gccaggagat gtataaccgt tatggcgaaa gtgcctatga agacggttat cgcatttaca
    17881 ccaccatcac ccgcaaagtg cagcaggccg cacagcaggc ggtacgtaat aacgtgctgg
    17941 actacgacat gcgccacggc tatcgcggcc cggcaaatgt gctgtggaaa gtgggcgaat
    18001 cggcgtggga caacaacaaa attaccgata cgctgaaggc gctgccgacc tatggtccgc
    18061 tgctgcctgc cgcagtcacc agcgccaatc ctcaggaagc aacggcgatg ctggcagacg
    18121 ggtcgaccgt cgtattgagt atggaaggtg ttcgctgggc gcgcccttac cgttcggata
    18181 ctcagcaggg accgacgccg cgtaaagtga ccgatgttct gcaaacgggt cagcaaatct
    18241 gggttcgtca agttggcgat gcatggtggc tggcacaagt gccggaagtg aactcggcgc
    18301 tggtgtcgat caatccgcaa aacggtgccg ttatggcgct ggtcggtggc tttgatttca
    18361 atcagagcaa gtttaaccga gccactcagg cactgcgtca ggtgggttcc aacatcaaac
    18421 cgttcctcta caccgcggcg atggataaag gtctgacgct ggcaagtatg ttgaacgatg
    18481 tgccaatttc ccgctgggat gcaggtgccg gttctgactg gcaaccgaag aactcaccac
    18541 cgcaatatgc cggtccaatt cgcttacgtc aggggctggg tcagtcgaaa aacgtggtga
    18601 tggtacgcgc aatgcgggcg atgggcgtcg actacgctgc agaatatctg caacgcttcg
    18661 gcttcccggc acaaaacatt gtccacaccg aatcgctggc gctgggttca gcgtccttca
    18721 ccccaatgca ggtggcgcgc ggctacgcgg tcatggcgaa cggcggcttc ctagtggacc
    18781 cgtggtttat cagcaaaatt gaaaacgatc agggcggcgt gattttcgaa gcgaaaccga
    18841 aagtagcctg cccggaatgc gatattccgg tgatttacgg tgatacgcag aaatcgaacg
    18901 tgctggaaaa taacgatgtt gaagatgtcg ctatctcccg cgagcagcag aatgtttctg
    18961 taccaatgcc gcagctggag caggcaaatc aggcgttagt ggcgaagacc ggcgcgcagg
    19021 agtacgcacc gcacgtcatc aacactccgc tggcattcct gattaagagt gccttgaaca
    19081 ccaatatttt tggtgaacca ggctggcagg gtactggctg gcgagcaggt cgtgatttac
    19141 aacggcatga tataggcgga aaaacgggta ccactaacag ttcgaaagat gcgtggttct
    19201 cgggttacgg tccgggcgtt gtgacctcgg tctggattgg ctttgatgat caccgtcgta
    19261 atctcggtca tacaacggct tccggagcga ttaaagatca gatctcaggt tacgaaggcg
    19321 gtgccaagag tgcccagcct gcatgggacg cttatatgaa agccgttctt gaaggtgtgc
    19381 cggaacagcc gctgacgccg ccaccgggta ttgtgacggt gaatatcgat cgcggcaccg
    19441 ggcaattagc taatggtggc aacagccgcg aagagtattt catcgaaggt acgcagccga
    19501 cacaactggc ggtgcacgag gtgggaacga ccattatcga taatggcgag gcacaggaat
    19561 tgttctgatt aaaaaggcgc ttcggcgcct ttttcagttt gctgacaaag tgcacttgtt
    19621 tatgccagat gcgggtgaac gcgttatccg gccaacaaaa aattgaaaat tcaataaatt
    19681 gcaggaactt cgtaagcctg ataagcgttg tgcatcaggc aaacttcacg catttacact
    19741 cgcccctgcc ctttcaacca ttcgcgcacg aggaacagcg cgctgacatt acgcgcttcg
    19801 ttgaagtcag gggcttccag caaatccatc atatgcgcca gcggccagcg cacctgtggt
    19861 aacggctctg gctcatcgcc ttccagtgat tccgggtaga gatcttgcgc taccacgata
    19921 ttcattttgc tggaaaagta agacggtgcc atgctgagct tcttcaaaaa agtcagatcg
    19981 ttcgcaccaa atccaacctc ttcttttagc tcgcggttag cggcttcaaa gacgctttca
    20041 cccggatcaa ttaatccttt cgaaaaacct aattcgtagg attcagttcc cactgcgtat
    20101 tcgcggatca ggatcaggtg atcgtccaca atcggcacaa tcatcactgc ttcccggttg
    20161 gttggacgca ttcgttcata aacacgccgc acgccattgc tgaactccag atccacgctt
    20221 tcgacggtaa acagtcggga acgggctaca gtttcaacat tcagaatggt gggtttttgt
    20281 aatgatttgc tcatcgtggg atctatgctg tgaaatcagc ggttattgtg cgatatcgga
    20341 cacgctttcg gcaatgtgaa ttgcgcgtta tttacattta tgtaacttaa taaataatct
    20401 tcctcaaatc aaattaaaag tcaataggtt gaaataactc caggaatttg ctgatattcc
    20461 gccttcagag ggtttgctat gatcagcggt tactgtgatg tgcttaatga tgctcaagtt
    20521 aaactccacg cttgccgata gccaaccgca gaatcatgta ttgtgtccgg tgcgactgac
    20581 cacgcctgac agactaagta agatggggaa agcatgagca ccattgtgat ttttttagct
    20641 gctttgctgg cctgctcact acttgcggga tggctgataa aagtgcgatc cagacggcgt
    20701 cagctgccct ggaccaacgc cttcgcggat gcgcaaacgc gtaaactcac atctgaagaa
    20761 cgtagcgccg ttgaaaatta tcttgagagc ctgacgcagg tattacaggt gcctggccca
    20821 acgggagcca gcgcggcacc gatctctctg gcgctgaatg ccgaaagcaa caacgtcatg
    20881 atgctgacac acgctatcac gcgttatggc atctctaccg acgatccgaa taaatggcgt
    20941 tactacctcg attcggtaga agtccacctg ccccctttct gggaacagta catcaacgat
    21001 gagaataccg ttgaactgat tcataccgat tcgctgccgc tggttatttc actcaacggt
    21061 catacgctgc aggagtacat gcaggaaact cgcggctatg ccttgcaacc tgttccgtca
    21121 acgcaggcgt cgattcgcgg ggaagagagc gagcaaatag agctgctcaa tattcgcaaa
    21181 gaaacgcacg aagagtatgc gctgagtcgt ccgcgcgggc tgcgcgaagc gttgctgatt
    21241 gtcgcctcct tcatgatgtt ctttttctgc ctgattaccc cggatgtctt tgttccgtgg
    21301 ctggcaggcg gcgcgttact gctgctgggc gcaggtctgt gggggctatt cgcgcccccg
    21361 gcaaaatcct ccctgcggga aattcattgt ctgcgcggta caccccgtcg ttggggattg
    21421 tttggcgaaa acgatcagga acagatcaac aatatttcac tcggtattat cgacctggtc
    21481 tatcccgcac actggcagcc atacattgct caggatctcg gtcaacaaac cgatatcgat
    21541 atctatctcg accgccacgt agtgcgtcag gggcgatatc tttcgctgca tgatgaagta
    21601 aaaaactttc cgttacagca ctggctgcgc agtacgatta tcgctgcggg ctcgctactg
    21661 gtgctgttta tgctgttatt ctggatcccg ctggatatgc cgctgaaatt caccctctcg
    21721 tggatgaaag gcgcacagac cattgaagcc actagcgtaa aacaactggc tgacgctggc
    21781 gtgcgggttg gcgatacatt gcgtattagc ggtacgggaa tgtgtaatat tcgcacttcc
    21841 ggtacctgga gcgcgaaaac caattcacct tttttaccat ttgactgctc gcagatcatc
    21901 tggaacgacg cccgctcatt gccattacca gaatctgaac tggtcaacaa agcgacggca
    21961 ttgaccgaag cagttaatcg ccagctgcac cctaaaccgg aagatgaatc tcgcgtcagt
    22021 gcctcattac gttcagcaat tcaaaaatcc ggtatggtat tgcttgatga ttttggcgac
    22081 attgttctga agacagcaga tctgtgttct gccaaagatg actgtgtgcg actgaaaaat
    22141 gcgctggtca atctcggcaa cagtaaagac tgggacgcgc tggtaaaacg cgccaacgcc
    22201 gggaaactcg atggcgtgaa tgtgttatta cgcccggtga gtgcggaatc gctggataac
    22261 ctggtggcca cctctaccgc gccgtttatc acgcatgaaa cggcgcgagc ggcacaatca
    22321 ctaaacagtc cggcccccgg cggattcctg attgtcagcg acgaaggcag cgattttgtt
    22381 gatcagccct ggccttcggc atcactttac gactatccgc cgcaagaaca gtggaacgct
    22441 ttccagaaac tggcacaaat gctgatgcat acgccgttta acgccgaagg tatcgtcaca
    22501 aaaatcttca ctgacgccaa tggtacgcag catattggcc ttcatccgat cccggatcgt
    22561 tccggcctgt ggcgctatct cagcaccacg ctgctgctac tgacgatgct gggtagcgcc
    22621 atttataatg gcgtacaggc ctggcgtcgt taccagcgcc atcgtactcg catgatgaag
    22681 attcaggcct attatgaaag ctgcctgaac ccgcaactga tcaccccttc agaaagcctt
    22741 atcgaataac acgtttgcgc ggcaggttat gctaccctgt cgcgcaaatt gcttcactct
    22801 ggagatttcc ctcatgcata tcaacattgc ctggcaggac gtagataccg ttctgctgga
    22861 tatggacggc acgttgctcg acctcgcctt cgataactat ttctggcaaa agctggtgcc
    22921 tgaaacatgg ggcgcgaaaa acggagttac gccacaggaa gcgatggaat atatgcgcca
    22981 gcaatatcac gacgtacagc atacgctaaa ctggtactgt cttgattact ggagtgagca
    23041 actgggtctg gatatctgtg cgatgaccac cgagatggga ccgcgtgccg tactgcgtga
    23101 agataccatt ccgtttcttg aggcactgaa agccagcggt aaacagcgaa ttttgctcac
    23161 caatgcgcat ccgcacaacc tggcggtaaa acttgagcat accggtctgg acgcacacct
    23221 tgatttatta ctttccaccc acacatttgg ttatccgaaa gaggatcagc ggttatggca
    23281 tgcggtggcc gaagctacgg gtcttaaagc tgaaagaacg ctgtttattg atgacagcga
    23341 agcgattctc gatgctgccg cgcaatttgg tattcgttac tgcctcggtg tgactaatcc
    23401 tgactccggg attgccgaga aacagtatca acgccatccg tcactgaatg actaccgccg
    23461 cctgatcccc tcgctaatgt gaaggagatg ccatgaaaga aaaacctgct gttaaggttc
    23521 gactggataa atggctatgg gctgcccgtt tttataaaac ccgcgcgctg gcccgtgaaa
    23581 tgattgaagg cggtaaggtg cattacaacg ggcagcgcag caagccaagc aaaatcgtcg
    23641 agctgaatgc cacgctcact ctgcgccagg gaaatgacga acgcacggtg attgtaaagg
    23701 cgattactga acagcgtcgc cccgccagcg aggcagcctt gctgtatgaa gagactgcgg
    23761 aaagtgtaga gaaacgcgaa aaaatggcgc tggcacgtaa acttaatgcc ttaaccatgc
    23821 cgcatccgga ccgacgcccg gacaaaaaag agcgccgcga cctgttacga tttaaacacg
    23881 gcgacagtga ataactgtca cctgcaagag agatgattat gccgcaacat gaccaattac
    23941 atcgctatct gtttgaaaac tttgccgttc gcggcgaact ggtaaccgtt tcggaaaccc
    24001 tgcaacagat ccttgagaat cacgattatc cgcagcccgt taagaacgtg ctggcagaac
    24061 tgctggttgc gaccagcctg ttaaccgcta cgttgaagtt tgatggtgat atcaccgtac
    24121 agctgcaagg cgacggtccg atgaatctgg cggttattaa cggtaacaac aaccagcaga
    24181 tgcgcggtgt ggcgcgcgtg cagggcgaaa ttccagaaaa tgccgacctg aaaacgctgg
    24241 tcggcaatgg ttacgtggtg atcaccatca ccccgagcga aggcgaacgc tatcagggcg
    24301 tggttggtct ggaaggtgat accctggcgg cctgcctgga agattacttt atgcgttctg
    24361 aacagctgcc gacgcgcctg tttattcgca ccggcgacgt agacggcaaa ccggctgcag
    24421 gcggaatgtt gttgcaggta atgcctgcgc aaaatgccca gcaggacgac tttgaccacc
    24481 tggcgacgct aaccgaaacc atcaaaaccg aagaactgct gactttaccg gcaaacgaag
    24541 tgttgtggcg tttgtatcac gaagaagagg tgacggttta cgatccgcag gatgtggagt
    24601 tcaaatgcac ctgctcgcgt gaacgttgcg ccgatgcgct gaaaacgctg cctgatgaag
    24661 aagtcgatag catcctggcg gaagatggcg aaattgacat gcattgtgat tactgcggta
    24721 accactatct gttcaatgcg atggatattg ccgagatccg caacaacgcg tctccggcag
    24781 atccgcaagt tcattaatga gtatgtccgg cagagaggac tctgccggat tttacgacta
    24841 atacacctga tgcgctttgt cactcattca gattcacatc cagcctttca ccatccacta
    24901 caagatcgcg ccacgcttta ggcctcgtcc ccagctttcc atccttcgca gctgtgagta
    24961 atttttcttt cgttattttg cgcggaagta aactcatatc aagcgcgtcc cattcttttc
    25021 tggttgagtc aaagccatag acaatgactc tgtcatcatt aaaagcaaac aggatccgct
    25081 ccgcctgacc atcactattc agatcttgct cgaccagtac gcaggcgtct tttccaatac
    25141 aggtcatcac gttatagcga tcctggatta aggccgacca gaatgtcgca tccggtttac
    25201 cagaacctgg ggcaattaac acattatcgg ctaatacttt ttccgatacc tgttcctgaa
    25261 gatgttgctc tccatctaac gccatcagca gatcccgcgc gcgtttcggg tctttcatat
    25321 actcggcatc gcttttcagc gactcaagcg ccgcacgtcc atagcgaccg ctctgttcga
    25381 gcatgtagat agttacctgg tctggcgtgt ttttgccgct ctgataacgc gccatatgac
    25441 tgttcacgct aatgcgcata ctgtccagca ccggcgaatt aagaagcacc agtattacca
    25501 acaccaataa cgaaaccaca aggttcactt tgccctgaag atcaagggga ttttgccctt
    25561 tacgccagac gatactgaca aaatacccca gcgaccatac cagtaacacc agcactgcca
    25621 gcgcgccctg caagcggtcg gcagtccagc cgtactgagc gacccgcagc cataacgccc
    25681 aggcggcgac gaacacatac agcggagcaa ccagcaaagc ggttttaatc aggcaacgca
    25741 atggccccgt ccagggaagt gacgcttttt gcggatcgcg gacaatagcc attaagatca
    25801 attgcaaaaa ggccagcgtt aacagcaacc cagcggcgga aatgtggcga gaaatcgcgc
    25861 tcaggcccgt aaacggcagg gtgataataa acatcagggt tagcaatgat actaacggca
    25921 gcaaccccgt ggcgattaac gtgaacaact tttgaataga gtcgattaaa cgtgactgtg
    25981 tacgcgcgag gatcaccgcc agtgcggtaa ccagacctaa cgtgagataa ataaacccgt
    26041 cggttgcaaa aaagagcgtt ttgaaaaacg tgatgccaac aagtttaaac aactctcccc
    26101 acagtaaaag taccagccac gtcaggccat tggcgagaaa aatcaccagt aatataagta
    26161 agttatgcca tactgactgg taaaaatagc gatagcggct actgtcattg cggatgcgaa
    26221 ggctttgttg tatccacggt agcaacaaca ttgccatcag cagcagatag cagccaaaat
    26281 cccaaagcgc cttttcagct ctccaggggg tcatgccatc agtttgccac ttcagccagt
    26341 cgctcattcc cagcgtggca ataaacacca gcgccagcca tccccaaagg cgattctgtt
    26401 taaaggagat cacggagaag agtaaaacgg atgaaaacgc gacggtcgca ggcacgccat
    26461 aaacaatcca actgagattt tttccggcaa gccaggcgat cagcaggtag caaaccaacc
    26521 cctgaagtaa tccggtagca atcatccccc agcgtgttgc cggtgaaagt tcaacgttat
    26581 ccatgttgtt tatccagaat caaaaggtgg gttaattatc gcatccgggc agtagtattt
    26641 tgcttttttc aaaaaataat caaaaaaagt tagcgtggtg aatcgatact ttaccgattg
    26701 aatttgcatc aatttcattc atgaatgcga ttccactcac aatattctcg ccatataaac
    26761 caagatttaa ccttttgaga acattttcca cacctaaaat gctatttctg cgataatagc
    26821 aaccgtttcg tgacaggaat cacggagttt tttgtcaaat atgaatttct ccagatacgt
    26881 aaatctatga gccttgtcgc ggttaacacc cccaaaaaga ctttactatt caggcaatac
    26941 atattggcta aggagcagtg aaatgcgcgt taacaatggt ttgaccccgc aagaactcga
    27001 ggcttatggt atcagtgacg tacatgatat cgtttacaac ccaagctacg atctgctgta
    27061 tcaggaagag ctggacccga gcctgaccgg ttatgagcgc ggggtgttaa ctaatctggg
    27121 tgccgttgcc gtcgataccg ggatcttcac cggtcgttca ccgaaagata agtatatcgt
    27181 tcgtgacgat accactcgcg atactttctg gtgggcagac aaaggcaaag gtaagaacga
    27241 caacaaacct ctctctccgg aaacctggca gcatctgaaa ggcctggtga ccaggcagct
    27301 ttccggcaaa cgtctgttcg ttgtcgacgc cttctgtggt gcgaacccgg atactcgtct
    27361 ttccgtccgt ttcatcaccg aagtggcctg gcaggcgcat tttgtcaaaa acatgtttat
    27421 tcgcccgagc gatgaagagc tggcaggttt caaaccagac tttatcatta tgaacggcgc
    27481 gaagtgcact aacccgcagt ggaaagaaca gggtctgaac tccgaaaact tcgtggcgtt
    27541 taacctgacc gagcgcatgc agctgattgg cggcacctgg tacggcggcg aaatgaagaa
    27601 agggatgttc tcgatgatga actacctgct gccgctgaaa ggtatcgctt ctatgcactg
    27661 ctccgccaac gttggcgaga aaggcgatgt tgcggtgttc ttcggccttt ccggcaccgg
    27721 taaaaccacc ctttccaccg acccgaaacg ccgcctgatt ggcgatgacg agcacggctg
    27781 ggatgatgac ggtgtgttta acttcgaagg cggctgctac gcgaaaacta tcaagctgtc
    27841 gaaagaagcg gaacctgaaa tctacaacgc tatccgtcgc gatgcgctgc tggaaaacgt
    27901 caccgtgcgt gaagatggca caatcgactt tgatgatggt tcaaaaaccg agaacacccg
    27961 cgtttcttat ccgatctatc acatcgataa catcgttaag ccggtttcca aagcgggcca
    28021 cgcaactaaa gttatcttcc tgacagcaga cgcctttggt gtgctgccgc cggtttctcg
    28081 tctgactgcc gatcaaaccc agtaccactt cctctctggc ttcaccgcca aactggccgg
    28141 gactgagcgt ggcatcaccg aaccgacgcc aaccttctcc gcctgcttcg gcgcggcgtt
    28201 cctgtcgctg cacccaactc agtacgcaga agtgctggta aaacgtatgc aggcggcggg
    28261 tgcgcaggcg tatctggtta acaccggctg gaacggcact ggcaaacgta tctccattaa
    28321 agatacccgc gctattatcg acgccatcct caacggttcg ctagataacg cagaaacctt
    28381 cactctgccg atgtttaacc tggcgatccc aaccgaactg ccgggcgtag acacgaagat
    28441 cctcgatccg cgtaacacct acgcttctcc ggaacagtgg caggaaaaag ccgaaacgct
    28501 ggcgaaactg tttatcgaca acttcgataa atacaccgac acccctgcgg gtgccgcgct
    28561 ggtagcggct ggtccgaagc tgtaatcagc tgatatctca ttgacccctt atccaaacgg
    28621 ataaggggtt tttttatatc taattagcta ccaatatgta cacttataat tatcaataga
    28681 ccgcaggatg cattatgttc acctttattg aactacaagg atttagcaaa cgccgccagt
    28741 tattgctacc tgataagtag ttccgggctt ttcaggaact actcattcaa gatcccactg
    28801 ccggtgacat tatcgcaggc acaggcagtt ttagaaaaat tcgctggaac cgttctggaa
    28861 tggggaaacg gggtggcatt cgaataattt attacaacgt aacaagcaat ggtcgaattt
    28921 atctggcgtt gatttatcct aaaaatgaac aggatgattt aacagaagaa caaagaaagg
    28981 cattgaaact attgtccgag aagctgcttt aatgttgaag ctccacaaat tccaccatgg
    29041 tcgaaatgcc aacggagaca ttgaaaacag atgaaagatg aattatttgc tgatcttctt
    29101 gccagcgcag aagaaatggt acgcatcgaa aagggtgaag aaacgcctga accagagcac
    29161 gtccatactt ttagcgaaat cgatgtaaaa gcgatccgtg aagcaacggg tttacgtcag
    29221 caagattttg ctgtcgccgt aggggtaagt tatgacctgg tcaaaagccg ggaaaccaaa
    29281 cgccgtcagc ctaccggagc accccgaaaa ttgctcctgc ttttgcaagc gaatcctttc
    29341 attatcaatc aattgaaaac aatctaaaaa cgggaggcac cttcacctcc cgttttttta
    29401 cccttctttt gtcatgccct gcgcccgcgt taccggcact ggcagccagg cgcgaatgga
    29461 aagcccgccc cgttcgctgg tgccaagctc cagcatcccg ttatggttat ccacgatacg
    29521 ctgcacaatc gccagcccta atcccgtgcc gctaatggtg cgcgcactgt caccacgaac
    29581 aaacggctgg aacaggtgct tacgttgttc cggcgcaata cccggaccgt catcttctac
    29641 ctggaaccag gcgcgattcg gctccgtgcc gctgctgact ttgatccagc cattgccgta
    29701 acgggcggcg ttgaccacca tattcgccac cgcgcgtttg atcgacagcg ggtgcatttt
    29761 cacctcaatg ctaccggggt aaagcgcagt ttcaatttcc cgctcatagc cactttcggc
    29821 agcaataacc tcaccgagta ctgcgttgag atccgccatt tccatcggca tctcctgccc
    29881 ggtgcgcagg tagtcgataa actgctcaat gatggcgttg cactcttcga tatctttatt
    29941 gatcgattct gccaggtagc catcctgctc gctcatcatc tcggtcgcca gacgaatacg
    30001 cgtcagcggc gtgcgcagat catggcttac ccccgccatc agcagcgtgc ggtcatccgc
    30061 cagttgctta acaccagccg ccatatggtt aaaggcgcgg gtaacggaac gcacctccga
    30121 agcgccatat tcacgcagcg gcggcggaat aatcccttta cccacctgca aggctgcgtg
    30181 ttcgagatcg accaacggtc ggttctggat acgaataaac agccacgccc cgcctatcgc
    30241 caatagcata atcgccaacg tataacggaa cagcggggag aaatcgccct gatgaatttc
    30301 ggttagcggc acgcgtaccc agatattggg cgacaaccag gttttcagcc agacgacagg
    30361 cgaacttttg ttgacctcga cgcgaacttc cgtcggaccg cccagttgct gcgccatctg
    30421 atggcttaag aattcatagt gttgcgccca acgcagacct gcctcttcgg cagcttcgtt
    30481 ggaatagaga gagatcccca gctcacggta gatctcccga cggaaagcgg gaggcacaac
    30541 caactgcgtg ccgtcctcca gttgcagttt gtcggtcatc aacatacgca cttcatacgc
    30601 gaggacttta ttaaactgct ggaggctcgg caaaatcgcg aagttcagca ccaccagata
    30661 agtcgtcacc aggctgacga acagcaaggt gacgatgagc aataacgtac gggcaaatga
    30721 acttcgtggc gagaagcgca atcgcctcat gctttagaac cgtccggtac aaagacgtag
    30781 cccagacccc agacggtctg aatgtaacgc ggatgcgccg gatcttcttc caccatgcgg
    30841 cgcagacgcg aaatctgcac gtcgatggag cgttccattg cggaatattc acgaccacgg
    30901 gcaaggttca tcagcttatc gcgggagagc ggctcacgcg gatggctgac cagtgccttc
    30961 agtaccgcaa actcaccact ggtgagcggc atcggctcgt cttcgcggaa catttcgcgc
    31021 gtaccgaggt taagtttgaa cttaccgaaa gcaattaccg cctcttcctg tgacggtgcg
    31081 cctggcagtt cgttcgcctg acgacgcagc accgcacgaa tgcgggccag cagttcacgc
    31141 gggttaaacg gttttggaat gtaatcgtca gcgccaatct ccaggccaac gatacggtcc
    31201 acttcttccc ctttcgccgt caccataatg atcggcatcg gattgctctg gctacgaaga
    31261 cgtcggcaaa tcgacaagcc atcttcacca ggtaacatca aatccagtac cataagatgg
    31321 aaagattcac gagtcagcag gcgatccatc tgttctgcat tagcgacgct tcgaacctgg
    31381 aagccttgtt cggtgagata acgttccagc agcgcgcgca ggcgcatgtc gtcatcaacc
    31441 accagaatct tgtagttctc ttgcattgtt tgtactccca aaggttcgca acaatttgta
    31501 agcgtgtatt cttaaaaaag ctcacgttcg tcaccagcta aatctggtat gaatttcagc
    31561 ctaaattgtt acaaagcata ttaaacagca gcttaagtat acaatttatt cggcgaaaca
    31621 ttattgattc tgttgatatg atcacgttat acccaatgtg cgcattatca aacagacaaa
    31681 gggaatcaac gagatgaaaa cgcccctggt tacccgggaa gggtatgaaa aactcaaaca
    31741 agagcttaat tatctctggc gtgaagaacg cccggaggtc acaaagaaag tgacctgggc
    31801 cgcaagtctg ggcgaccgca gcgaaaatgc tgactatcag tataataaaa agcgcctgcg
    31861 tgaaatcgac cgtcgcgtgc gctatctcac taaatgcatg gaaaatctca aaatcgtcga
    31921 ttactccccg cagcaggaag gcaaagtctt ttttggcgcg tgggtggaga ttgaaaacga
    31981 cgatggcgtg actcaccgtt tccgtattgt cggctacgat gaaatttttg gccgtaaaga
    32041 ttacatctct atcgattccc cgatggcccg cgcattgctg aaaaaagaag tcggcgatct
    32101 ggcggtggtg aatacccctg ccggggaagc gagctggtat gttaatgcta tcgagtacgt
    32161 gaaaccgtaa ggaagagtct taacctcctg ccgatggctg gcatttttgc cagccagtcc
    32221 gtataactat cccctgattt ttgatccgaa aagatgaact caaaccatga tgaatgattc
    32281 gttctgccgc attattgcgg gtgaaattca ggcgcgcccg gaacaggttg acgctgccgt
    32341 tcgcctgctt gacgaaggga ataccgtgcc gtttatcgca cgttatcgta aggaaatcac
    32401 cggcggtctg gatgacacgc agctgcgtaa tctggaaacg cgtctgagct atctgcgcga
    32461 gctggaagag agacgtcagg cgatcctcaa gtccatttcc gagcaaggca aactcaccga
    32521 tgatctggcg aatgccatca acgccaccct aagcaaaacc gaactcgaag acctctacct
    32581 gccctacaaa cctaaacgcc gcacccgtgg gcaaatcgcc attgaagcag ggcttgagcc
    32641 gttggccgat ctgctgtgga gcgatccgtc acacacgcca gaagtcgccg ctgcacaata
    32701 tattgatgcc gataaaggcg tggcagatac caaagccgcg ctggacggcg cgcgctatat
    32761 cctgatggaa cgttttgccg aagatgctgc gctgctggcg aaagtgcgtg attatctgtg
    32821 gaagaacgcg catctggtct caaccgtggt gagcggtaaa gaagaggaag gggcaaaatt
    32881 ccgcgactat ttcgatcatc acgaaccact gtcaacggtg ccttctcacc gcgcactggc
    32941 gatgttccgt gggcgtaacg aaggcgtact tcagctttcg ctgaatgccg atccgcagtt
    33001 cgatgagccg ccaaaagaga gctattgcga gcaaatcatc atggatcatc tcggcctgcg
    33061 cctgaacaat gccccggcgg atagctggcg caaaggcgtg gtgagctgga cgtggcgcat
    33121 caaggtgctg atgcatctgg aaaccgaact gatgggcacc gtgcgcgaac gcgcggaaga
    33181 tgaagcaatc aacgtctttg cccgtaacct gcacgatctg ctgatggcgg ccccagccgg
    33241 actacgtgca acgatgggcc tcgatccggg tctgcgtacc ggggtaaaag tagcggtggt
    33301 cgatgcaact ggcaaactgg tggcgaccga taccatttat ccgcataccg ggcaggccgc
    33361 aaaagcagcg atgaccgttg ctgcgctgtg tgaaaagcat aacgttgaac tggtggcgat
    33421 tggcaacggt acagcttccc gcgaaaccga acgtttctat ctcgacgtgc agaagcagtt
    33481 cccgaaagtg accgcgcaga aagtgatcgt cagcgaagct ggcgcgtcgg tttactcggc
    33541 ttccgagctg gcagcacagg aattcccgga tctcgacgtt tcgctgcgtg gcgcggtgtc
    33601 tatcgcccgt cgtttgcagg atccgctggc ggagctggtg aaaatcgatc cgaaatctat
    33661 cggcgtaggt cagtatcagc atgacgtcag ccagacgcag ctggcacgca aactggatgc
    33721 agtagtagaa gactgcgtaa acgccgttgg cgtcgatctc aacaccgctt ctgttcctct
    33781 gttaacccgc gtggcgggcc tgacgcgcat gatggcgcaa aacatcgtgg cctggcgcga
    33841 tgagaacggc cagttccaga accgtcagca actgttaaaa gtgagccgtc tggggccgaa
    33901 agccttcgag cagtgcgcgg gcttcttgcg cattaaccac ggtgataacc cgctggacgc
    33961 ttctaccgtt cacccggaag cctatccggt ggtggaacgc attctggcag caacacagca
    34021 ggcactgaaa gatctgatgg gtaacagcag cgaactgcgt aacctgaaag catctgactt
    34081 tactgatgag aaattcggtg tgccgacggt aaccgacatc atcaaagagc tggaaaaacc
    34141 gggtcgcgat ccgcgtccgg aatttaaaac cgctcagttt gccgatggcg tcgagacaat
    34201 gaacgacctg caaccgggta tgatcctcga aggtgcggtg accaacgtca ccaactttgg
    34261 cgcgtttgtc gatattggtg tgcatcagga cggcctggtt cacatctctt cattgtcgaa
    34321 caagtttgtg gaagatccgc ataccgtggt gaaagcgggc gacattgtga aggtgaaagt
    34381 gctggaagtg gatcttcagc gtaaacgtat cgccctgacc atgcgtctgg acgagcagcc
    34441 tggcgaaacc aacgcccgtc gcggtggcgg taacgatcgc ccgcaaaaca accgcccggc
    34501 agccaaaccg cgtgggcgtg aagcgcagcc tgccggtaat agcgcgatga tggatgcgct
    34561 ggcggcggca atgggcaaaa aacgttaaac gcccggactg gcccctaaac ctgcgcgatt
    34621 aggcgttcac gccacatctg gcaacgaacg ccttgcctga cataaaagtg ccggagaata
    34681 tctccggcct ttttattcca caagcaaact cataatatat cccgttaata tttatcactt
    34741 cattaacaac tgaaacctta attaaacatt aaccagccct aataattcgt catgcgaatt
    34801 atattttcgc tgcgatataa ccttgagcca tatcaacatt gagtcagatt attattcaaa
    34861 ccaacattca cacacatttt aagtattgct gatagaaacc attctcatta tcattgtact
    34921 gttgattatt taatctctcc ttcgttggca aatcatctgg tctcatgtcg ctgtcaaacg
    34981 ccccatgagg tagttatcca gttaatgaga aacaagtagg cacctatgca atacactcca
    35041 gatactgcgt ggaaaattac tggcttttcc cgtgaaatca gcccggcata tcgccaaaaa
    35101 ctgctttctc ttggcatgtt acctggctcc tcttttaatg tggtgcgcgt cgctccactc
    35161 ggcgacccca ttcatatcga aacccgtcgt gtgagcctgg tattacgcaa aaaagatctg
    35221 gccttattag aagtggaagc ggtttcctgt taatacggtg ataacaacaa tgaaaaaatt
    35281 aaccattggc ttaattggta atccaaattc tggcaagaca acgttattta accagctcac
    35341 tggctcacgt cagcgtgtag gtaactgggc tggcgttacc gtcgaacgta aagaagggca
    35401 attctccacc accgatcatc aggtcacgct ggtggacctg cccggcacct attctctgac
    35461 caccatctca tcgcagacct cgctcgatga gcaaatcgcc tgtcactaca ttttgagtgg
    35521 cgacgcagac ctgctgatta acgtggtgga tgcgtctaac cttgagcgta acctgtacct
    35581 gacgctgcaa ctgttggaac tcggcattcc atgcatcgtg gcgctgaaca tgctcgacat
    35641 tgccgagaag caaaatattc gcattgaaat tgatgcgctg tcggcgcgtc ttggctgccc
    35701 ggtgatcccg ctggtttcaa cccgtggtcg cggtattgaa gcgctcaagc tggcgattga
    35761 tcgctataaa gctaacgaaa atgtggaact ggtgcattac gctcagccgc tgctcaacga
    35821 agcagattca ctggcaaaag tgatgccttc cgacatcccg ctgaaacaac gtcgctggct
    35881 gggcctgcaa atgctggaag gcgatatcta cagccgcgcc tacgccggtg aagcgtcgca
    35941 gcatctggat gccgccctcg cccgtctgcg taatgagatg gacgatccgg cgctgcacat
    36001 tgccgatgcg cgttaccagt gcattgctgc catctgtgat gtggtaagca acaccctgac
    36061 ggcagaaccc agccgtttca ccactgcggt agataaaatc gtgctcaacc gtttcctcgg
    36121 tctgccgatt ttcctctttg tgatgtacct gatgttcttg ctggctatca acatcggcgg
    36181 ggcgttacag ccgctgtttg acgtcggctc cgtagcactc tttgtgcatg gtattcagtg
    36241 gattggctac acgctccact tcccggactg gctgactatc ttcctcgccc aaggcctggg
    36301 cggcggtatt aacaccgtgc tgccactggt gccgcagatc ggcatgatgt acctgttcct
    36361 ctccttcctt gaggactccg gctatatggc gcgtgcggcg tttgtgatgg accgtctgat
    36421 gcaggcgctg ggcttgccgg ggaaatcctt tgtaccgctg atcgttggtt tcggttgtaa
    36481 cgtaccgtcg gtaatgggcg cacgtacgct tgatgcaccg cgtgaacgtc tgatgaccat
    36541 catgatggca ccgtttatgt cctgcggcgc gcgtctggct atcttcgcag tattcgcggc
    36601 tgccttcttc gggcagaacg gtgcgctggc ggtcttctcg ctgtatatgc tgggtatcgt
    36661 aatggcggtg ctgacgggcc tgatgctcaa gtacaccatc atgcgcggtg aagcgacgcc
    36721 gtttgtcatg gagctgccgg tctaccatgt accacacgtt aaaagcctgg ttatccagac
    36781 ttggcagcgt ctgaaaggct tcgttctgcg tgctggtaaa gtgattatca ttgtcagcat
    36841 tttcctgagc gccttcaaca gcttctcgct gagcggaaaa atcgtcgata acatcaacga
    36901 ctcggcgctg gcttccgtca gccgggtgat caccccggtc ttcaagccga ttggcgtaca
    36961 tgaagataac tggcaggcaa cggttggcct gtttacaggt gccatggcga aagaagtggt
    37021 ggtgggtacg ctcaacaccc tctacaccgc agaaaatatt caggacgaag agttcaatcc
    37081 ggcggaattt aacctcggtg aagagctgtt cagtgcggta gatgaaacct ggcagagcct
    37141 gaaagacacc ttcagcctta gcgtactgat gaaccccatt gaagccagca aaggcgacgg
    37201 cgaaatgggt accggggcga tgggcgtgat ggatcagaaa ttcggtagcg cagctgccgc
    37261 atacagctac ctgattttcg tcctgctgta cgtaccgtgc atctcggtga tgggggctat
    37321 cgcgcgtgaa tcaagccgtg gctggatggg cttctccatc ctgtgggggc tgaatattgc
    37381 ttactcactg gcaacattgt tctatcaggt cgccagctac agccagcatc caacttacag
    37441 cctggtgtgc attctggcag ttatcctgtt taacatcgtg gttatcggtc tgctgcgccg
    37501 cgcgcgtagc cgggtagatg tcgaactgct ggcaacgcgc aaatcggtaa gcagttgctg
    37561 tgcggccagt accactggcg attgccacta aggacacgac aatggcttcg ctcattcaag
    37621 tgcgtgattt actggcgtta cggggccgta tggaagcggc ccagattagc cagacactga
    37681 acactccgca gccaatgatt aacgccatgc tgaaacagct agaaagtatg ggaaaagcag
    37741 tgcggattca ggaagaacct gacggctgtc tttccggcag ttgtaaaagc tgcccggaag
    37801 gaaaagcctg tctgcacgaa tggtgggcgt tgcgttaacc ttgtttcact gcctgttttg
    37861 aaagcccggt atgcgtttgc atccgggctt ttttgcgtgc ggctttccat aaaaatgcaa
    37921 cccttgcagc gcgacgtaag ttcctctgaa agcgtctcgc agggatgaaa actcgctaat
    37981 gcacaggtgt ggagtggcgc gtagagtcgc ggcattcaaa taacaggtga aggaacgcca
    38041 tgagcaaaaa gcagagttcc accccacacg atgcgttacg ccattgccgc aatgcaaaat
    38101 caccttgatg ccggatacaa aacattgccg atggtggtgc cattgttgtt ttatcacggt
    38161 attgaaagcc cctatcccta ttcgctgtgt tggctggatt gtttcgccga tcccaacctg
    38221 gcaaggcagc tttatgcctc tgcatttccg ctgattgatg tcaccctcat gcctgatgat
    38281 gaaatcatgc tgcaccgacg tatggcgcta ctggagttaa ttcaaaaaca tattcgtcaa
    38341 cgcgacctga tggggctggt agagcaaatg gcctgcttat taagtagtgg atacgctaat
    38401 ggcagacaaa tcaaagggct gtttaattac atactgcaaa ccggcgacgc ggtacgtttt
    38461 aacgatttta tcgatggtgt ggccaaacgt tcaccaaaac acaaggtgag tttaatgact
    38521 attgcggaaa gattgcggca ggaaggggaa caatccaaag ccttgcatat agcaaaaata
    38581 atgcttgaat ccggagtccc tcttgcagac atcatgcgct ttaccggggt gtcagaagaa
    38641 gagttggctg cagcgagcca gtaaagttct gtcccgccat ttcgaaagcc acctacaccc
    38701 tctgcttcaa cgccaccagc agatgacaaa actcgtccgg atgtgaaata aacggcgcat
    38761 gggccgcttt ggcgaagata tatgattcgc tgtgaggcca aagtttatcc agcatcggca
    38821 ccactttgcg cggcaccaga ccgtcgagat agccatacaa tcgcaaaaac ggcatgggca
    38881 cgttttgcag cggctgacgg agatcgaccg ttttcaggat ttccagcccg ccattaagca
    38941 cgtcaacctc cggcatcggt aacgccagaa cggttttctt caacgcccgc gcatcctggc
    39001 gcgccgtttc agtacccatg gtttgtaacg ccaggaaccg ctccactgta cgctgaaaat
    39061 catcactgag ttgctgctga aatcccgcca gcacgtccgg ttttatcccc ggccactcgt
    39121 cgcgagcact aaaacaaggt gacgacgcca cggtgaccag cgccagaacc cgctcgggat
    39181 gggttaacgc aatctggctt gccaccagcc cgcccagact ccagcctaac caaatggctt
    39241 tatctggtgc ctgtcgcagc acgacttcgg ccatatcagc aagtgatagc gcaccaaatc
    39301 cccggctacg cccgaagccg ggcaggtcaa caaggtgcag cgtaaaatgc gagctaagtt
    39361 cctcgtcaat gcaacgccac acttcggcat tcagtcccca tccgtgcagc agcacaagat
    39421 gaacattccc ctgacctttg gtctgccacc agatgttatt catccgctat tgttctcttt
    39481 ttacttacaa ggatgaacat atgctaacag taccgggatt atgctggcta tgccgaatgc
    39541 cactggcgtt aggtcattgg gggatttgtt cagtctgctc tcgcgccgcc cgtacagata
    39601 aaacgttatg cccacaatgt ggattacccg ccacacactc tcatcttccc tgcggtcgct
    39661 gcctgcaaaa accgctgccc tggcaaagac tggtcacggt cgccgactat gcgccgccat
    39721 taagtccgct cattcaccag cttaagtttt cccggcgcag tgaaatcgcc agcgccctgt
    39781 cacgtctgtt actattggaa gtcttacacg ctcgtcgcac caccggcttg caactgcctg
    39841 atcgcatcat cagcgttccg ttatggcagc gccgtcactg gcgtcgggga tttaatcaga
    39901 gcgatttgct gtgtcagccg ttatcacgct ggctacactg ccaatgggat agcgaagccg
    39961 tcacacgaac acgggccacc gcgacccagc attttcttaa cgcccggcta cgcaaacaca
    40021 acctgaaaaa tgcctttcgc cttgaattgc ctgtgcaagg tcgccatatg gtgattgtgg
    40081 atgatgtcgt taccaccgga agtaccgtcg cagagattgc gcagttgctt ttacgcaatg
    40141 gtgcggcgac tgtccaggtc tggggccttt gtcgaacctt gtagagcctc gatgatgggc
    40201 gtattataac caactaaaat agtcaactat taggccatta ctatgatccg tatttccgat
    40261 gctgcacaag cgcactttgc caaactgctg gcaaatcagg aagaagggac acaaatccgc
    40321 gtatttgtga ttaaccctgg cacgcctaac gctgaatgtg gtgtttctta ttgcccgccg
    40381 gacgctgtgg aagccaccga caccgccctg aaatttgacc tgctgaccgc gtatgttgat
    40441 gagttaagcg ctccttacct ggaagatgca gagatcgatt ttgttaccga ccagttgggt
    40501 tcgcagctga cgctgaaagc gcctaacgcc aaaatgcgta aagtggctga cgatgcgccg
    40561 ctgatggagc gcgtggagta tatgctgcaa tcacagatca acccacagct tgctggtcac
    40621 ggtggtcgcg tttcgctgat ggaaatcacc gaagacggtt acgccatttt gcaatttggc
    40681 ggtggctgta acggttgttc catggtcgac gtcaccctga aagaagggat cgaaaagcag
    40741 ttgctgaacg aattcccgga gctgaaaggt gtacgcgatc tcaccgaaca ccaacgcggc
    40801 gagcactctt actactaagt tctctcccct tttgtcgatt gcccgatgtg acgctaaagc
    40861 gtcttatcgg gcctgtacgt tgcacgagag tctgacgtta gcgcagaatt acctcagcaa
    40921 atccaatctc ttcgtttccc cacgataata tgaccaacct ctcataattt aaatttaccc
    40981 cgctctggtg attctcaagc acgtaatgtt acccgtatca ttctcatggg caccaaacac
    41041 actcataaca tctgactaca ctaactcgtt ttaggggtca tcagccgtgg tgccgtaaca
    41101 tctctgttcc cagttgggac aaccaggatt ttccgtcaga aacctgacgt tacccataac
    41161 aaattaaagg ccaggtaaat catgccatta gtcattgttg ctatcggtgt aatcttgtta
    41221 ttactcctga tgatccgctt caaaatgaac ggcttcatcg ctctcgtcct cgtggcgctt
    41281 gctgttggat taatgcaagg aatgccgctg gataaagtta ttggctccat caaagctggt
    41341 gtcggcggaa cgctcggtag ccttgccctg atcatgggtt ttggtgcgat gctgggcaaa
    41401 atgctggcag actgcggtgg tgcacaacgt atcgctacca cactgattgc caaatttggt
    41461 aaaaaacaca tccagtgggc ggtggtactg accggtttta ccgttggttt tgccctgttc
    41521 tatgaagtgg gtttcgtgct gatgctgccg ctggtgttta ccatcgcggc ttccgcgaat
    41581 attccactgc tgtatgttgg tgtaccaatg gcggctgcac tgtctgtgac ccacggcttc
    41641 ctgccaccgc atccgggtcc gactgcaatt gccaccattt tcaatgccga tatgggtaaa
    41701 actctgctgt acggtactat tctggcaatc ccgaccgtga ttctcgccgg tccggtttac
    41761 gctcgcgtgc tgaaaggtat cgataagccg atcccggaag gtctgtacag cgcgaaaacc
    41821 ttcagcgaag aagagatgcc gagctttggc gtcagcgtct ggacctctct ggtgccggtg
    41881 gttctgatgg cgatgcgtgc gattgccgaa atgatcctgc cgaaaggtca cgctttcctg
    41941 ccggtggcgg agttcctcgg tgacccggta atggccacgc tgattgccgt gctgattgcg
    42001 atgttcacct ttggtctgaa ccgtggtcgt tcaatggatc agattaacga cacgctggtt
    42061 tcttccatca aaatcatagc gatgatgctg ttgattatcg gtggtggcgg tgcgttcaag
    42121 caggtgctgg tagacagcgg cgtggacaaa tacattgctt ccatgatgca cgaaaccaac
    42181 atttctccgc tgctgatggc ctggtcgatt gctgccgtac tgcgtatcgc gctgggttcc
    42241 gcaaccgtcg cggcaatcac tgcgggtggt atcgcggcac cgctgattgc aacgacgggt
    42301 gttagcccgg agctgatggt tattgcggtt ggttccggta gtgtgatttt ctctcacgtg
    42361 aacgatccgg gcttctggct gttcaaagag tactttaacc tgactatcgg cgagaccatc
    42421 aaatcctggt cgatgctgga aaccattatc tcggtgtgcg gtctggtagg ctgtctgctg
    42481 ctgaatatgg tgatttgata atactgatgg ccggatgcgg cgtgaacgcc ttatccggcc
    42541 tacgattcgg cattaattag taggccggat aagacgcgtc aagcgtcgca tccggcatca
    42601 accgcactct acttcttctt cgctgccgct ctgcgccgtc tgtccaaatc cttcagcaac
    42661 ttgttcacac catcatcggc aaacatcgac tcaagcgttg cggaaagttt acgtcgccag
    42721 tttttatact ggtaactggt gccgggaata tttaccggat ctgccatatc cagccagtct
    42781 tccggctgta gccctaacag ggcgctgttg ctatcggcga tgtagcgctg taagccacga
    42841 ttcagcgtcg gcgtcatcga catcaacgat gccttatgcc cggcacgttt tggcagacaa
    42901 ccatatttat gcagagcatc cagtagtcct tgcttcgcca gttcgcgatc ctgatacaga
    42961 ccgcgcagta ccacttcatc cggatacaac cccagcgttt tgcccagcgt tagatccccg
    43021 ctctcccagt agccgcgtag cgtaggcagg tcgtgtgtcg ccgcaaccgc catcgactgc
    43081 tctgggtacg ctttcggcgc gcggaatgtc ttctcgtggt cgttttcgaa atagagcact
    43141 ttgtaggagt acacaccgct gctgcgcagc ttaccgacaa tctctaccgg tacggtaccg
    43201 agatcttcac caatcaccat acagcgatga cgcttacttt cgagtgccag aatcgagagc
    43261 agatcatcca ccggatagtg aacatatgcc ccctgatctg ccgtctcgcc atacggtatc
    43321 caccacaaac gcagcatcga catcacatgg tcaattcgta atgcgccgca gttttgcata
    43381 ttggcacgca acagctcgat aaacggttca taggcacgcg cggtgataat atgcgggtcc
    43441 attggcggca atccccagtt ctgccccaac gggccgagga tatccggcgg tgctccaacc
    43501 gacgctttca ggcaatacaa ctcccggtcg caccaggttt ctgctccacc ctctgctaca
    43561 ccaacggcca ggtcacgata caagccaatc ggcatttcat agccctggct tatctcccag
    43621 caggcggcaa actggctgta agccagccac tgcaaccaga gataaaaatc aacgtcatcg
    43681 cgatgttctt cgcagaactg gcgcacttct ggtgaatcca cgttctgata catctctggc
    43741 catgcaggcc agccccagcg catttcgtcc tctttcactt gctgggcatg tagtgcgtca
    43801 aacgccgcct gccagaacag gctgtcgcct tgctctgcaa caaactggcg aaacgcggcc
    43861 atctgttcat catcacgttg cgcgaaacct ttccacgcca ttcgtaatgc tgtcattttt
    43921 agggcggtaa ccgtggagta atcgacccag tcggcatccc gcgcctgttg cagcgtctgt
    43981 tgcgtggtcg gcaacttcca ccaagcctga gcctcttcgc taagatggaa atcttcaacg
    44041 gcgttaacgt cgatataaat cacattcagc caacggcgag aagacgggct atacgggctg
    44101 gaactttccg gatttgccgg atagagcgca tgaatcgggt tcagaccgat gaacgacccg
    44161 ccacgttttg ccacatccac cagcatcgct ttgagatcgc caaaatcccc aataccccag
    44221 tttttttccg atcgcagtgt ataaagctga acgcaggcac cccacagctt ttgtttgttc
    44281 agcaacgcct gcggttcgta acagcgtttc ggggcgacaa tcacccggca atgcgcgcgc
    44341 tggtcgtcct gggtaagcgt cagcgtgtga taaccttccg gcagcttcgt cggtagattg
    44401 aacgctttgc cccccatcac atggcctttg tactgctttc cttcttcggt ggtcagcagc
    44461 cagctatatt cgccgctgcc ctccaccacc atcggcattt ttttgccgct ggtataaacc
    44521 atgacattcg ggactggcgt taccgccact ttcgtggcgg tacgttgatg catcgcgtca
    44581 agcaaacgcc gtttggtttc ggcgctaatc gactgcggtt taccgtgggc attgatgtaa
    44641 ttggggctga tccccgccgc cagcgcggca ttatccagac gtttgctttc catcgagctt
    44701 ccttagcgtt ttgcctgcca gatacgagcc tgataatcgc gaatagagcg atccgagcta
    44761 aacataccgc agcgggcggt attgaggatc gccgcgcgag tccaggcctc ctggtcgcgg
    44821 tacagcacat ccacctgctt ttgtgcctct acataagcgg cgaaatctgc catcaccaga
    44881 tacggatcgc cgccctgttt gccgatactg tgcagcatct ggtcgaaggc atgcttatcg
    44941 ccgtcgctgt atttaccgct ttccagctct ttcaatactg cgtccagcac cttatctttc
    45001 ttccgccatt tcaccgggtc gtagcctttg gccagaattg ccttcacttg ttccacggta
    45061 tgaccaaaaa taaagatatt ttcttcaccg actttctcag cgatttcaac gttcgcacca
    45121 tccagcgtac cgacagtaag cgcaccattg agcgccagtt tcatattgcc ggtaccggaa
    45181 gcttctttac ctgcagtcga aatttgttcg gagatatccg ccgccgggat cagtttttcc
    45241 gccgccgaaa cacaataatc cggcaagaaa accaccttca acttgtcgcc aaccagcgga
    45301 tcgttgttga tcacgtcagc cactttgttg atcgcaaaga taatattttt cgccaggtag
    45361 tagcccggtg ctgctttcgc gccgaagagg aagacgcgcg gtacgcggtc agactgcggg
    45421 ttttcacgaa tttctttgta caacgccaga atatgcagca gattcagatg ctggcgtttg
    45481 tactcatgca gacgtttgat ctgaatatcg aaaatcgcct gtggattgat ctcaataccg
    45541 gtacgaactt tcacaaactc tgccagacgg actttattcg cctgcttgat ctcgcgatat
    45601 tgctgacgga atttcgcatc atcagcgaat ttttccagat tgatcagttg atcgagatcg
    45661 ttagcccact cttttttcag tgatttatcc aacagagccg ccagtgccgg gttgcactgt
    45721 ttgatccagc gacgtggggt aataccgttg gtgacattat ggaatttgtt cggccatagc
    45781 tggtgatatt ccgggaacag atctttcacc accagatctg agtgcagcgc cgcaacaccg
    45841 ttcaccgcga aaccgccaac cacacacagg ttcgccatat gcacttgttt gtcgtgtacc
    45901 accgccagtt tggcccacac tttttcatcg cccggccagg ttttctccac cagcgtttta
    45961 aagcgagtat taatttcgtt aataatctgc atgtggcgcg gcagtaagcc tttcaccagt
    46021 ttcacatccc agcgttccag cgcttctggc atcagggtat ggttggtgta agcgaaagtt
    46081 ttgctggtga tggcccaggc gtcatcccag ctcatctggt gctcatcgat cagcacgcgc
    46141 agcagttctg gaatcgcgat agttgggtgg gtatcgttca gctgaataac ttcgtaatcc
    46201 gccagttcgt gcagtttacg ccccgccaga tgatggcgac gcaaaatatc cgctaccgag
    46261 caggcacact ggaagtattg ctgcatcagg cgcaactttt taccggcagt atggttgtcg
    46321 tttggataga gaactttggt cagtttttcc gcgttgatgc cctgctgttc ggcacgcagg
    46381 aaatcaccgt cgttaaattt agtcagatca aacggatgcg catgcgtcgc ctgccacaga
    46441 cgcagtggct gcgccacgcc attacgatag ccgacaacgg ggagatccca cgcttgaccg
    46501 gtaatggtaa actccggctc ccagcgtcca tctttcgtca ctttaccgcc aatccctacc
    46561 tgcacatcca gtgcttcgtt gtggcggaac cacgggtagt taccgcgatg ccagtcatcc
    46621 ggcgcttcaa cctgtttgcc atcgacaaat gactggcgga acaagccata ttgataatta
    46681 aggccgtagc cagtagctga ctgcccgaca gttgccattg agtcgaggaa gcacgccgcc
    46741 agacgtccca gaccaccgtt ccccagcgcc gggtcgatct cttcttccaa caggtcagtc
    46801 aggttgatgt cataagcctt caacgaatcc tgtacatcct gataccagcc gagattcaac
    46861 aggttgttgc ccgtcaggcg accaatcaaa aactccattg agatgtagtt aacatgtcgc
    46921 tgattcgcca ctggcttggc gaatggctga gcacgcagca tttcggccag tgcttcgctc
    46981 actgccagcc accactggcg aggagtcatt tcagccgcag aatttaagcc ataacgctgc
    47041 cactgacgtg aaagcgcttc ctgaaattgc ttatcgttaa aaataggttg tgacatagga
    47101 gttccacttt tcttagattt tcaacacaac gttatcgcta gtttgccagg ctcgatgttg
    47161 accttcctca tcctgcgggg gattaggcag ggaggagttg cggggatgag caaggaaatg
    47221 tgatctcgac cacttaaagc tagtgcaatc cacaggatta gcagcaaatc aatgccatac
    47281 cgcgcagaaa atctgtatct aagtgcaaaa aatggccgtt gcgtattttc aaaaagcgga
    47341 aggtaactct ataaattaag taaaggagtg aaacagtttc acaagtaaaa tatcaagtgt
    47401 gctccatctc attcttaata gatttattaa gatcatcttt ttagatggca ctttcatcag
    47461 aaatgaagaa gaaacccttg cttaaatgaa actgatgaac ataaggaaaa taccgtacaa
    47521 cccagtattc acgctggatc agcgtcgttt taggtgagtt gttaataaac atttggaatt
    47581 gtgacacagt gcaaattcag acacataaaa aaacgtcatc gcttgcatta gaaaggtttc
    47641 tggccgacct tataaccatt aattacgaag cgcaaaaaaa ataatatttc ctcattttcc
    47701 acagtgaagt gattaactat gctgattccg tcaaaattaa gtcgtccggt tcgactcgac
    47761 cataccgtgg ttcgtgagcg cctgctggct aaactttccg gcgcgaacaa cttccggctg
    47821 gcgctgatca caagtcctgc gggctacgga aagaccacgc tcatttccca gtgggcggca
    47881 ggcaaaaacg atatcggctg gtactcgctg gatgaaggtg ataaccagca agagcgtttc
    47941 gccagctatc tcattgccgc cgtgcaacag gcaaccaacg gtcactgcgc gatatgtgac
    48001 acgatggcgc aaaaacggca atatgccagc ctgacgtcac tcttcgccca gcttttcatt
    48061 gagctggcgg aatggcatag cccactttat ctggtcatcg atgactatca tctgatcact
    48121 aatccagtga tccacgagtc aatgcgcttc tttattcgcc atcaaccaga aaatctcacc
    48181 cttgtggtgt tgtcacgcaa ccttccgcaa ctgggcattg ccaatctgcg tgttcgtgac
    48241 cagctgctgg aaattggcag tcagcaactg gcatttaccc atcaggaagc gaaacagttt
    48301 tttgattgcc gtctgtcatc gccgattgaa gctgcagaaa gcagtcggat ttgtgatgat
    48361 gtttccggtt gggcgacggc actgcagcta atcgccctct ccgcccggca gaatactcac
    48421 tcagcccata agtcggcacg ccgcctggcg ggaatcaatg ccagccatct ttcggattat
    48481 ctggtcgatg aggttttgga taacgtcgat ctcgcaacgc gccattttct gttgaaaagc
    48541 gccattttgc gctcaatgaa cgatgcactc atcacccgtg tgaccggcga agaaaacggg
    48601 caaatgcgcc tcgaagagat tgagcgtcag gggctgtttt tacagcggat ggatgatacc
    48661 ggcgagtggt tttgctatca cccactgttt ggtaacttcc tgcgccagcg ctgccagtgg
    48721 gaactggcgg cggagctgcc ggaaatccac cgtgccgccg cagaaagctg gatggcccag
    48781 ggatttccca gcgaagcgat tcatcatgcg ctggcggcag gcgatgcgct gatgctgcgc
    48841 gatattctgc ttaatcacgc ctggagtttg ttcaaccata gcgaactgtc gctgctggaa
    48901 gagtcgctta aggccctgcc gtgggacagt ttgctggaaa atccgcagtt ggtgttattg
    48961 caggcgtggc tgatgcaaag ccagcatcgc tacggcgaag ttaacaccct gctggcccgc
    49021 gctgaacatg aaatcaagga catcagagaa ggcaccatgc acgcagaatt taacgctctg
    49081 cgcgcccagg tggcgattaa cgatggtagt ccggatgaag cggaacggct ggcaaaactg
    49141 gcactggaag agctgccgcc gggctggttc tatagccgca ttgtggcaac ctcggtgctg
    49201 ggtgaagtgt tgcactgcaa aggcgaattg acccgctcac tggcgttaat gcagcaaacc
    49261 gaacagatgg cacgccagca cgatgtctgg cactacgctt tgtggagttt aatccagcaa
    49321 agtgaaattc tgtttgccca agggttcctg caaaccgcgt gggaaacgca ggaaaaagcg
    49381 ttccagctga tcaacgagca gcatctggaa cagctgccaa tgcatgagtt tctggtgcgc
    49441 attcgtgcgc agctgttatg ggcctgggca cggctggatg aagccgaagc gtcggcgcgc
    49501 agcgggattg aagtcttgtc gtcttatcag ccacagcaac agcttcagtg cctggcaatg
    49561 ttgattcaat gctcgctggc ccgtggtgat ttagataacg ctcgtagcca gcttaaccgt
    49621 ctggaaaacc tgctggggaa tggcaaatat cacagcgact ggatctctaa cgccaacaaa
    49681 gtccgggtga tttactggca aatgaccagc gataaagccg ccgctgccaa ctggttgcgt
    49741 catacggcta aaccggagtt tgcgaacaac cacttcctgc aaggtcaatg gcgcaacatt
    49801 gcccgtgcac aaatcttgct gggcgagttt gaatctgcgg aaattgtcct cgaagaactc
    49861 aatgaaaatg cccgcagtct gcggttgatg agcgatctca accgtaacct gttgctgctt
    49921 aatcaactgt actggcaggc cggacgtaaa agtgacgccc aacgtgtgtt gctggacgca
    49981 ttaaaactgg cgaatcgcac cggatttatc agtcattttg tcatcgaagg cgaagcgatg
    50041 gcgcaacaac tgcgccagct tattcaactc aatacgctgc cggagctgga acagcatcgc
    50101 gcacagcgta ttctgcgaga aatcaatcaa catcatcggc ataaattcgc ccatttcgat
    50161 gagaatttcg ttgaacgtct gctaaatcat ccggaagtgc cagagctgat ccgcaccagc
    50221 ccgttgaccc aacgtgaatg gcaggtcctg gggctgattt actccggtta cagcaatgag
    50281 caaattgcag gcgagctgga agtggcggca accaccatca agacgcatat ccgcaatctg
    50341 taccaaaaac tcggtgtcgc ccaccgtcag gccgcggtac aacacgcgca gaaactgctg
    50401 aagatgatgg gatatggggt atgagtttag ccggataatg cgccagatcc ggcttacatc
    50461 tttacatcat tcaatgctca cccgcgttac gccatctgtt tctatcagac taaaccgcac
    50521 tggcaagaaa cgctccacca ccgcgatatt ggtctgcaga tggcatgagg gatgggcgac
    50581 cgtaaattct cccgcccccg ccagcgccat cggtagcacc agttggtcgg cgagatattc
    50641 ccccaccgcc gccgggcttg ccaggtagcg tttcacctct ttcaccaact gtgccgcgac
    50701 cacctcggca ctgacgcgct tttcaccgac gacaaaaaag cgttcggtga tattttcact
    50761 ttcgacttca agcgagacgg tattacccgg cccctggtcg cgcggcaggt tatgaatatt
    50821 ctgttcatgc aaggaaaaac tacccgccag tgtagcgatt tcacgctcag caacatggcg
    50881 cggcacacca gccagtagca cctctccacg catctgcaca atgttcccgc gctcgccaag
    50941 ttgcaagcta ttaaacgatg ccaccggtga gacttccgtt gccaccacgc cgcctccggc
    51001 aggataaaaa ccgtgacgta acagcgtggt ttgctgatga actcctattt tcgccagcag
    51061 cggctccagc acccggcgga taaaatccgc aggcggggcc gacgggttat cggtgccgcc
    51121 gctcacttca acacgcgaag gtccatcggc aaaccacagc gcgggcagca ccgtttgcag
    51181 caccagcgta caacttccgg cgctaccgat agcaaagcgg taatcgccgc cgcgcacggt
    51241 gccaggtcgg aagaccagac gctgcgaccc cagctcagcg ccttccaccg ttgccctaca
    51301 aatttccgca gccgctttta ccgcggtcag atgttggcgc aacagccccg gtttcgcccg
    51361 cccggcacga atgccggtga tggtaaatgg ctggccggtt atcatcgaca ggctcagcgc
    51421 cgagcgcagg atctgcccgc cgccttcgcc ctgtgcgcca tccagcgcaa tcatcctttt
    51481 catcatttat ccttttacgc acaccacctg acgcagggta tagataactt ccaccagatc
    51541 gctttgtgcc gccatcaccg catcaatatc tttatacgcc atcgggattt cgtcgatcac
    51601 ctcggcatct ttacggcact ccacatgcgc ggtggcacgg atttgatctt ccacactgaa
    51661 cagttttttc gctttagtac ggctcattac ccgcccggca ccgtggctgc acgaacagaa
    51721 cgactcttca tttcccagcc cacggacgat aaagcttttc gcccccatcg aaccgggaat
    51781 aattccatat tgaccagcac gcgcagacac cgcgccttta cgcgtcacat agatctcttc
    51841 gccaaagtgc tgttcttttt gcacatagtt gtggtgacag ttgatctctt ccatcgccag
    51901 cgtttgtggc tgttttaccg ttttctgcgt aacgctctgc aacgccgtta ccacgttttc
    51961 catcatcgca tcgcggttaa ggctggcaaa aagctgcgcc caggctacgg ctttcaggta
    52021 atcatcaaag tattccgtac cttccataaa gtacgccagg tcacgcgatg gcaacgtttc
    52081 aagcgtttcc tgcatctctt tttgcgccag atcgataaaa taagttccga tggcgttacc
    52141 aattccgcgt gaaccggagt gcagcataat ccacacctga tccgactcat caaggcaaat
    52201 ttcaataaag tggttacccg tacccagcgt tcccaggtgt ttatagttat tagtgttaag
    52261 gaaacgcgga tatttttgcg ttaaccactg ataaccggcc tccagctcag cccatttcgc
    52321 atcgacgtta acaggcggat tttcccatgc gcctttatca cgtttacaac gcccggtagt
    52381 acgtccgtgc ggtacggccg tttcaatcgc ctgacgcagc tctgccaggt tttctggcag
    52441 gtccgccgcc gttaacgcgg tacgcagtgc gttcattcca cagccaatat ccacgcccac
    52501 cgccgccgga ataatcgccc ctttggtcgg gatcacgcta ccaatggtgg aacctttacc
    52561 caggtgtaca tcaggcatta ccgcaatatg tttgaaaata aacggcatct tcgcggtatt
    52621 aataagttgc tgacgcgcat cggcttctac cggcacgcct ttggtccaca tttttaccgg
    52681 agcattttca gtggtcagta attcgtaatt cattttgttt ttctcttttc gttgtttgct
    52741 gtcctgataa ttgcaaccgt cgtgccagaa aatcaaataa gcatcttaat tttttaattc
    52801 attgttttta aaaatattat atttttacgt ccgtaaacgg agatttcccg caaagccgat
    52861 ttaccgataa tgaaatctcg tcttttataa ggatatctaa gatgcgtaaa acagtggctt
    52921 ttggctttgt cggaacggtg ctggattatg ccgggcgcgg cagtcagcgc tggtcaaaat
    52981 ggcgtccgac actctgtcta tgccagcaag aatcgttagt tatcgatcgg ctggaattat
    53041 tgcacgacgc ccgctcgcgc tcgctattcg aaacgcttaa acgcgatatc gccagcgttt
    53101 cgccagaaac agaagtggtg ggcgttgaga tagaactgca taacccttgg gatttcgaag
    53161 aggtttacgc ctgcctgcat gatttcgccc gtggttacgc gttccaacct gataaagaag
    53221 agtatttaat tcacatcacc accggcaccc acgtcgcgca gatttgctgg tttctgctgg
    53281 cagaagcgcg ttacctgccc acccggttga tacaatcttc accaccgcgc aaaaaagagc
    53341 ggcccgatag ccctggcgca gtgacgatta tcgatctcga tttaagccgt tataacgcca
    53401 tcgccagccg ctttgccgag gaacgccagc aaacgcttga ttttcttaag tccggtattg
    53461 ccacgcgtaa ctcccacttc aaccgcatga ttgagcagat tgaaaaagtg gcgataaaat
    53521 cccgcgcacc gatcctgctt aacggcccga ccggtgcagg taagtcattt ctggcacgac
    53581 gtatcttcga gctaaaacag gcgcggcatc agtttagcgg cgcgtttgtg gaagtgaact
    53641 gcgccaccct gcgcggcgat accgccatgt cgacgctgtt tggtcatgtg aaaggcgcgt
    53701 ttaccggggc gcgggaatcg cgtgaaggat tattacgcag cgccaacggc ggaatgttgt
    53761 ttcttgatga gattggcgaa ctgggcgcag acgaacaggc aatgctgctg aaagccattg
    53821 aagagaaaac cttttacccg tttggcagcg atcgccaggt gagcagtgat tttcagctta
    53881 tcgccggaac ggtgcgcgat ttgcgccagt tggttgccga aggcaaattt cgtgaagacc
    53941 tgtacgcgcg aataaatctc tggaccttca ccttgccagg attacgccaa cgccaggaag
    54001 atattgaacc gaacctggat tatgaagtgg agcgccacgc ctcactcact ggcgacagcg
    54061 tgagttttaa caccgaagcg cggcgtgcct ggctggcctt tgcgacctct ccccaggcaa
    54121 catggcgcgg taactttcgc gaactttctg ccagcgtcac gcggatggcc acctttgcca
    54181 ctagcggacg catcacactg gatacggtgg aagatgagat caaccgtctg cgctataact
    54241 ggcaggagag tcgcccctcc acgcttacgg cgttgcgggc gcagaggcag aaaacatcga
    54301 tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgccagg caaagtcgct
    54361 ttctgctgcc ggacgtgaac tgtttgacat ttcgcgccag ggtaaagcca gcgtcaatga
    54421 cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acatgggaag caatgcagga
    54481 tcagcacagc tccagttgaa tatgatggtc cttcagcacc tgcatcacgc tcgcaggcgg
    54541 catcgtgtcg gtgtagacag catcgaccat gctgatgcta cccatattga ccattgcgtt
    54601 acggccaaat tttgagtggt cgacaaccag cataacgtgg cgcgagttct caatgatggc
    54661 gcgtttggtg cgaacttcgt ggtaatcgaa ctccagtagc gagccgtcgc tatcgatgcc
    54721 gcttatcccc agaatgccga aatcgaggcg gaactgggag ataaaatcga gcgttgcttc
    54781 gccaatgatc ccgccatcac ggctacgcaa ttcaccaccg gcaagaatga tacggaaatc
    54841 ctcttttacc atcagcgtgt tagcgacgtt gagattgttg gtcacaatgc gcaaattgct
    54901 gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga taaacagcgt
    54961 cgagccattg ggaatttgct ccgccacttt gcgggcgatg cgctcttttt cttcggtctg
    55021 ggtggccttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg ccgcaccgcc
    55081 atgatggcgc aggatcaggt tttgctctgc cagctcgttg agatcgcggc gaatagtctg
    55141 cgggctaacg gagaaatgct ctaccagctc ctcggtgctg acataaccct gctgtttaac
    55201 cagttcgata ataccgttgt gacgttgtgt ttgtttcatt gataaatccc tggaattatt
    55261 ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg gctaacccgg
    55321 cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg gcgacaatcc
    55381 agatcaacgc aaagataatt aacccacgtt gcaggtaaat gccactttgc ggatcgcgtt
    55441 cgccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc ccgccaaacc
    55501 acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg agagtaatga
    55561 caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac caccaccaga
    55621 gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg aagtaacgcc
    55681 agaactcaaa tttcagcgtc ggatcgaatg gccaggccag ccataacatc acttcttgat
    55741 cgccgagaat ttgcatggcg ataaacacca ccacgcaggc gatcatcatc acccaggtta
    55801 ccggacctgc gcgttcacgc aaggcagcaa agaaaggata acggcgataa tgcaggccac
    55861 tgtcggtatg accggactgc caactggccg ccagataacg cggatctgcc gggtttccga
    55921 gaaaacgcgc cagctccgcc cgcacgcgtt cggcctggga ctcatccgcc agccagacat
    55981 cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg taatcaacaa
    56041 acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt gctgtcgctt
    56101 attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct ctgcgggaaa
    56161 ctggcgctgc caggcttcaa agccgccatc aatgctgtag accacatcgt agccctgttg
    56221 cagcagatac tgcgccgcgc ctttgctgct attaccgtgg taacacatca ccatgactgg
    56281 cgtatcgaag tcgttatcac gcataaacgc gcccagtgtg tcgttagtta aatggaaagc
    56341 ctgcgccgca tgccccattg cgaaactctg tggatcgcga atatcgacca gcaccgcctc
    56401 tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact gatccatgcg
    56461 tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc gaagcggcag
    56521 ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt acccaaagcg
    56581 cgattctttg ctaatatgtt cgataacgaa catttatgaa ctttaacgaa agtgaatgag
    56641 ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg tgctggtatc
    56701 gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca ggatctcgct
    56761 tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta ccttgagcac
    56821 tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctact gaaaatggcc
    56881 ccgcatatcg ccttcccgat gcgttttcgc ctgccgcatc gtccgcatct acgcccggcg
    56941 tggatgattc gtattggtct gtttatgtac gatcatctgg gtaaacgcac cagcctgccg
    57001 ggatcaaccg gtttgcgttt tggcgcaaac tcagtgttaa aaccggaaat tatgcgcgga
    57061 ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa cgcccagatg
    57121 gtggtgcgta aaggcggtga agtgcttact cgtactcgtg ccacgtctgc tcgccgcgaa
    57181 aaaggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata tacatggcag
    57241 gcgcgcggcc tggttaatgc taccggcccg tgggtgaaac aatttttcga cgacgggatg
    57301 catctgcctt caccttatgg cattcgcctg atcaaaggca gccatattgt ggtgccgcgt
    57361 gtgcataccc agaagcaagc ctacattctg caaaacgaag ataaacgtat tgtcttcgtg
    57421 atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata caaaggcgat
    57481 ccaaaagcag tgaagattga agagagtgaa atcaattacc tgttgaaagt gtataacacg
    57541 cactttaaaa agcagttaaa ccgggacgat atcgtctgga cctactccgg cgtgcgtccg
    57601 ctatgtgatg atgagtccga ctcgccacag gccattaccc gtgattacac ccttgatatc
    57661 catgatgaag acggcaaagc cccgctgctg tcggtattcg gcggtaagct gaccacctac
    57721 cgcaaactgg cggagcacgc gctggaaaaa ctgacgccat attatcaggg cattggtccg
    57781 gcgtggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg tgacgattac
    57841 gccgcccgcc tgcgccgccg ttacccgttc ctgaccgaat cgctggcacg tcactacgcg
    57901 cgcacttacg gcagcaacag cgaactgttg ctcggcaatg caggggcgat aagcgacctc
    57961 ggtgaaaatt tcggtcatga gttctacgaa gcggagctga aatatctggt cgaccacgaa
    58021 tgggtccgcc gcgccgacga tgccctgtgg cgtcgcacga aacaaggcat gtggctaaat
    58081 gcggatcaac aatctcgtgt gagtcagtgg ctgatggaat atacgcagca gaggttgtcg
    58141 ctggcgtcgt aaggatgatg cccggttgcg aagtgcaacc gggctatttg aattccttag
    58201 actattcatt gtaaataatt gaataacggc cccagagcta aaatcgccaa acttaagcac
    58261 gttacccaat cgccccactt cttcctatgg cttctatttt tgctacgcca ctggcaataa
    58321 tatcccaacg caaaaccaag ctgggcacta atcgggccga caaaatcagt ccacggctgc
    58381 ggccaaaaag gggaaaaatt caggggaagc cagacaaatg tcagaaagaa atttcggata
    58441 ttttccagca acgctgattt ctctggagaa atataaagaa tgaaattcga gagaattaac
    58501 cagaaataga ccggagaaaa gaccagccag caatgtagcc tgttttttaa ttcaggaggc
    58561 gttctgcggg agaattgcca accgatgcca cccattacac tgaaaaaaag tgcatattca
    58621 accaataact caagttgtaa ttcctgaaga gaaatacgca caaccaggag cggaaaggag
    58681 aatattattt ccgccagaaa agttaacgtg aatataagta cggctaaggc aataaccgtc
    58741 ggccatacca ataatggtac gataacaaaa ccgagaatta cagggggaat ataaagccgg
    58801 aatttaatca tttttatttt cctggtgatt cggatgatgc gtcatacatc gcgcataaac
    58861 actcatcaat catccagtcg tcttcccaga tctgatactg tgtgccct
//
LOCUS       DAZFFE010000016        58849 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFFE010000016 DAZFFE010000000
VERSION     DAZFFE010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.016"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(67..1827)
                     /gene="ycaO"
                     /locus_tag="ACHJ69_001190"
     CDS             complement(67..1827)
                     /gene="ycaO"
                     /locus_tag="ACHJ69_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO5014225.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTIYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWSFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1957..2649
                     /gene="ycaP"
                     /locus_tag="ACHJ69_001191"
     CDS             1957..2649
                     /gene="ycaP"
                     /locus_tag="ACHJ69_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO5014226.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     ELTKASRAKRVT"
     gene            2848..3936
                     /gene="serC"
                     /locus_tag="ACHJ69_001192"
     CDS             2848..3936
                     /gene="serC"
                     /locus_tag="ACHJ69_001192"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO5014227.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMSEWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAAMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4007..5290
                     /gene="aroA"
                     /locus_tag="ACHJ69_001193"
     CDS             4007..5290
                     /gene="aroA"
                     /locus_tag="ACHJ69_001193"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO5014228.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAESARELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRQGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIRGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDFIRITPPEKLKFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQPG"
     gene            5460..6224
                     /gene="ycaL"
                     /locus_tag="ACHJ69_001194"
     CDS             5460..6224
                     /gene="ycaL"
                     /locus_tag="ACHJ69_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO5014229.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGINTDLAISSGLNAYKAATL
                     SDADAKAIANQGCVEMDSGNKVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6397..7080
                     /gene="cmk"
                     /locus_tag="ACHJ69_001195"
     CDS             6397..7080
                     /gene="cmk"
                     /locus_tag="ACHJ69_001195"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO5014230.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7191..8864
                     /gene="rpsA"
                     /locus_tag="ACHJ69_001196"
     CDS             7191..8864
                     /gene="rpsA"
                     /locus_tag="ACHJ69_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO5014231.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9024..9308
                     /gene="ihfB"
                     /locus_tag="ACHJ69_001197"
     CDS             9024..9308
                     /gene="ihfB"
                     /locus_tag="ACHJ69_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO5014232.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9514..11778
                     /gene="ycaI"
                     /locus_tag="ACHJ69_001198"
     CDS             9514..11778
                     /gene="ycaI"
                     /locus_tag="ACHJ69_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO5014233.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGIVTLAFLTFFACVLAFIPV
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEIIATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQVWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGCWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAIWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPFILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCLPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     IDDGTNSILLTGDIEAPAEQKMLSRYWQQMQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11815..13563
                     /gene="msbA"
                     /locus_tag="ACHJ69_001199"
     CDS             11815..13563
                     /gene="msbA"
                     /locus_tag="ACHJ69_001199"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO5014234.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLMWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13560..14546
                     /gene="lpxK"
                     /locus_tag="ACHJ69_001200"
     CDS             13560..14546
                     /gene="lpxK"
                     /locus_tag="ACHJ69_001200"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO5014235.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PIPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAIKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            14583..15815
                     /gene="ycaQ"
                     /locus_tag="ACHJ69_001201"
     CDS             14583..15815
                     /gene="ycaQ"
                     /locus_tag="ACHJ69_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO5014236.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPHRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHAAEIAQLIQHIHDKGPVRSADFEHSRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHCVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQFFDFSYRRECYIPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     PQGLFTDCRAGWEIDPVA"
     gene            15867..16049
                     /gene="ycaR"
                     /locus_tag="ACHJ69_001202"
     CDS             15867..16049
                     /gene="ycaR"
                     /locus_tag="ACHJ69_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO5014237.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16046..16792
                     /gene="kdsB"
                     /locus_tag="ACHJ69_001203"
     CDS             16046..16792
                     /gene="kdsB"
                     /locus_tag="ACHJ69_001203"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO5014238.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKDLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLKRVRAEMR"
     gene            16946..17839
                     /gene="ycbJ"
                     /locus_tag="ACHJ69_001204"
     CDS             16946..17839
                     /gene="ycbJ"
                     /locus_tag="ACHJ69_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO5014239.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSAPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17816..18595)
                     /gene="elyC"
                     /locus_tag="ACHJ69_001205"
     CDS             complement(17816..18595)
                     /gene="elyC"
                     /locus_tag="ACHJ69_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO5014240.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPIADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDHVGYETLGRIWQWLKGSSGEPRQE"
     gene            18731..19516
                     /gene="cmoM"
                     /locus_tag="ACHJ69_001206"
     CDS             18731..19516
                     /gene="cmoM"
                     /locus_tag="ACHJ69_001206"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO5014241.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQILGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19513..20835
                     /gene="mukF"
                     /locus_tag="ACHJ69_001207"
     CDS             19513..20835
                     /gene="mukF"
                     /locus_tag="ACHJ69_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO5014242.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMVLR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20816..21520
                     /gene="mukE"
                     /locus_tag="ACHJ69_001208"
     CDS             20816..21520
                     /gene="mukE"
                     /locus_tag="ACHJ69_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO5014243.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            21520..25980
                     /gene="mukB"
                     /locus_tag="ACHJ69_001209"
     CDS             21520..25980
                     /gene="mukB"
                     /locus_tag="ACHJ69_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO5014244.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEFLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLNQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VIVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26241..28088
                     /gene="ldtD"
                     /locus_tag="ACHJ69_001210"
     CDS             26241..28088
                     /gene="ldtD"
                     /locus_tag="ACHJ69_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO5014245.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGVAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTIETAETKPMDKQTTSRSKPAPVVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNNREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28269..28817
                     /gene="mepK"
                     /locus_tag="ACHJ69_001211"
     CDS             28269..28817
                     /gene="mepK"
                     /locus_tag="ACHJ69_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO5014246.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28844..29491
                     /gene="gloC"
                     /locus_tag="ACHJ69_001212"
     CDS             28844..29491
                     /gene="gloC"
                     /locus_tag="ACHJ69_001212"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO5014247.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFLDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29543..30733)
                     /gene="aspC"
                     /locus_tag="ACHJ69_001213"
     CDS             complement(29543..30733)
                     /gene="aspC"
                     /locus_tag="ACHJ69_001213"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO5014248.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAALHKELIVASSYSKNFGLYNERVGACTLVAADSETVERAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(30919..32007)
                     /gene="ompF"
                     /locus_tag="ACHJ69_001214"
     CDS             complement(30919..32007)
                     /gene="ompF"
                     /locus_tag="ACHJ69_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO5014249.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEESSLGKGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(32610..34010)
                     /gene="asnS"
                     /locus_tag="ACHJ69_001215"
     CDS             complement(32610..34010)
                     /gene="asnS"
                     /locus_tag="ACHJ69_001215"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO5014250.1"
                     /translation="MSVVPVADVLQSRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(34179..35381)
                     /gene="pncB"
                     /locus_tag="ACHJ69_001216"
     CDS             complement(34179..35381)
                     /gene="pncB"
                     /locus_tag="ACHJ69_001216"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO5014251.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLENKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            35647..38259
                     /gene="pepN"
                     /locus_tag="ACHJ69_001217"
     CDS             35647..38259
                     /gene="pepN"
                     /locus_tag="ACHJ69_001217"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO5014252.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDVPLRLNGEDLKLVSVYINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAVELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(38454..39221)
                     /gene="ssuB"
                     /locus_tag="ACHJ69_001218"
     CDS             complement(38454..39221)
                     /gene="ssuB"
                     /locus_tag="ACHJ69_001218"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO5014253.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVA
                     LGLKGQWRDAARQALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWLKHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGHSEQPIRRHG"
     gene            complement(39218..40009)
                     /gene="ssuC"
                     /locus_tag="ACHJ69_001219"
     CDS             complement(39218..40009)
                     /gene="ssuC"
                     /locus_tag="ACHJ69_001219"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO5014254.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVLFALGLMWLTLIVAETISANSGIGYLAMNALEF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(40021..41166)
                     /gene="ssuD"
                     /locus_tag="ACHJ69_001220"
                     /pseudo
     CDS             complement(40021..41166)
                     /gene="ssuD"
                     /locus_tag="ACHJ69_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(41163..42122)
                     /gene="ssuA"
                     /locus_tag="ACHJ69_001221"
                     /pseudo
     CDS             complement(41163..42122)
                     /gene="ssuA"
                     /locus_tag="ACHJ69_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
     gene            complement(42115..42690)
                     /gene="ssuE"
                     /locus_tag="ACHJ69_001222"
     CDS             complement(42115..42690)
                     /gene="ssuE"
                     /locus_tag="ACHJ69_001222"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO5014255.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSTLKAQEILHGVFADDSQVIDYQHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            42927..43937
                     /gene="pyrD"
                     /locus_tag="ACHJ69_001223"
     CDS             42927..43937
                     /gene="pyrD"
                     /locus_tag="ACHJ69_001223"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO5014256.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KTYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQVMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            44111..44653
                     /gene="zapC"
                     /locus_tag="ACHJ69_001224"
     CDS             44111..44653
                     /gene="zapC"
                     /locus_tag="ACHJ69_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO5014257.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWMPMPGDAAYVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(44650..45759)
                     /gene="ycbX"
                     /locus_tag="ACHJ69_001225"
     CDS             complement(44650..45759)
                     /gene="ycbX"
                     /locus_tag="ACHJ69_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO5014258.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPLMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAGA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGICRVQ
                     LLEGEVTPLKKSAIGDDGTILCCSCVPKTALKLAR"
     gene            45857..47965
                     /gene="rlmKL"
                     /locus_tag="ACHJ69_001226"
     CDS             45857..47965
                     /gene="rlmKL"
                     /locus_tag="ACHJ69_001226"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO5014259.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWTQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVNDVAQLANPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMAA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRDFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            47977..49884
                     /gene="uup"
                     /locus_tag="ACHJ69_001227"
     CDS             47977..49884
                     /gene="uup"
                     /locus_tag="ACHJ69_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5014260.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELHNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            50014..51267
                     /gene="pqiA"
                     /locus_tag="ACHJ69_001228"
     CDS             50014..51267
                     /gene="pqiA"
                     /locus_tag="ACHJ69_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO5014261.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            51272..52912
                     /gene="pqiB"
                     /locus_tag="ACHJ69_001229"
     CDS             51272..52912
                     /gene="pqiB"
                     /locus_tag="ACHJ69_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO5014262.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     LLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKGKKDPEPKRAKQ"
     gene            52909..53472
                     /gene="pqiC"
                     /locus_tag="ACHJ69_001230"
     CDS             52909..53472
                     /gene="pqiC"
                     /locus_tag="ACHJ69_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO5014263.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGMQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            53727..53894
                     /gene="rmf"
                     /locus_tag="ACHJ69_001231"
     CDS             53727..53894
                     /gene="rmf"
                     /locus_tag="ACHJ69_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO5014264.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(53964..54482)
                     /gene="fabA"
                     /locus_tag="ACHJ69_001232"
     CDS             complement(53964..54482)
                     /gene="fabA"
                     /locus_tag="ACHJ69_001232"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO5014265.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTAND
                     LKVGLFQDTSAF"
     gene            complement(54551..56311)
                     /gene="ycbZ"
                     /locus_tag="ACHJ69_001233"
     CDS             complement(54551..56311)
                     /gene="ycbZ"
                     /locus_tag="ACHJ69_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5014266.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGGHYEVSGHTIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQTIYSEFEDTLQIVDAESVSQWCRWVTFTAKHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCQEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEDKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            56497..56949
                     /gene="matP"
                     /locus_tag="ACHJ69_001234"
     CDS             56497..56949
                     /gene="matP"
                     /locus_tag="ACHJ69_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO5014267.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDEL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(57027..58067)
                     /gene="ompA"
                     /locus_tag="ACHJ69_001235"
     CDS             complement(57027..58067)
                     /gene="ompA"
                     /locus_tag="ACHJ69_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO5014268.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IDNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEVAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQALSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(58423..58749)
                     /gene="sulA"
                     /locus_tag="ACHJ69_001236"
                     /pseudo
     CDS             complement(58423..58749)
                     /gene="sulA"
                     /locus_tag="ACHJ69_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
     gene            complement(58767..>58849)
                     /locus_tag="ACHJ69_001237"
     CDS             complement(58767..>58849)
                     /locus_tag="ACHJ69_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO5014269.1"
                     /translation="KSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT        14572 a        14494 c        15570 g        14213 t
ORIGIN      
        1 taaaaaaatg ccccgtaata taactcagac gaatcattaa gcctacattg cgtaggctat
       61 ttgattttat tttgcccaga atgctgcttt ggcgcgttgc agcttttcgt aggccttcaa
      121 caacgactga tgtgcagcaa acgcgtgcag atcgctatct actggttgca agccataaaa
      181 cgccgcttcg ccgctcattg ccgcactggc ggcttccacg gcatctgcgc cgtacatgcg
      241 aacaaacgca ttcagatatt gcagcggctg gcgatcttct tcctgcgcca gtaataacaa
      301 cgtttgcagg cagcgataat aattggcgcg ttccgggctg aatactgatg agttaaactc
      361 catcgtccat tcggtccaga ccagagcctg ttccagatcg ccaccagcca gcgccagcat
      421 ggcttttaat tcaccgatac gcagagtgta ccagccgtta tccgacccgg tcgccagacc
      481 caacagttca cgcacgcggg taaagtcatc aaaaccttct tcatccagtt gctcgatgag
      541 gttcaggtaa tcttcctttt cccactcgct acctggtagc gaaagaatcg tttcacgtaa
      601 atggctgccc atactgttat tcgccagcca cagatcttca gccggataaa tatcggacat
      661 gccaggcacg ataatacggc aagcgtagac acccagatgc tcgtaatcgg caatataaac
      721 ttctttatct tcctttttga agatagccat cagcgtggcg aactcttctt ccgtggtgcc
      781 ggagaagctc cagtcaacaa acggataatc ggcatcctgc ttgaacaggt cccaggagat
      841 taaaccgctg gaatcgataa agtgcgtttc gaggttggta tgttcagcga cttcttcatc
      901 atcgaaggtt ggtggagtaa acacatccag atctttcagg ccacgacctt gcagcaactc
      961 ggtcacggta cgttccagtg caacgccaaa atcaggatgc gcaccgaatg aggcaaagca
     1021 ggtaccgtta gcaggattga acagtaccac gcaaatcacc ggatactggc cgccaagcga
     1081 accgtcataa gcgaagatcg ggaaaccttc cgcttccagc gtttcgatcg cttcaactac
     1141 tgctggataa cgtgccagca cgtctgccgg gatttccggc aagctgatgc tttcagcaat
     1201 aatgcggttt ttcacgtaac gttcgaaaac ttcggacaac ccctgaacgc gtgcttcgtt
     1261 acgggtattg ccagcagaca taccgttaga aacgtacaag ttaccaatga tattcatcgg
     1321 aatataaatg gtctgattgt cggactggcg cgtaaacggt agaccgcaaa taccacgatc
     1381 ttcgttaccg gattgcaggt caatcagcat actaccggtc agttcattct ccggatcgta
     1441 aaatgcgcgc agacggtcat cgagcagccc ttctggtaca tcgtcatttt cggtcagtgg
     1501 gaaccatttt tcgttgggat aatgcacgaa tgggccgttg gcgatggttt cgcccagcca
     1561 gaagtccgca aaaaagtagt tggttgagag acgttcgaaa tattcaccga gggcagaagc
     1621 cagcgccgct tttttggttg cgcctttacc gttggtaaaa cacagtgcgc actctttgtc
     1681 gcgaatatgt acagaccaga cattaggcac gggattcagc caggaggcct cttcaatctg
     1741 aaaaccgagg tctgaaagtt tttgctggaa gcgtgcgatg gaatcttcca gagcggcatc
     1801 tttgccgggg ataaatgttt gcgtcatgaa aatcacttta gtcgtacgga aagcgcgcaa
     1861 taatacgggt tttatctcaa aggtgctatc accgccgcca cgctgatgaa ctgttagcta
     1921 tgcttttagc ggattaacta cgataagagc ataaaaatga aggcgtttga tctccaccgt
     1981 atggcattcg ataaagtgcc ttttgatttc cttggcgaag ttgcactacg tagtctttat
     2041 acctttgtac tggtcttttt gttcctcaaa atgaccggaa gacgcggtgt gcggcagatg
     2101 tcgctgtttg aagtgttaat cattctgaca ctgggttcag cggcgggaga tgtggcgttt
     2161 tatgatgatg tgccgatggt cccggtactt atcgtcttta ttactctggc gttgttatac
     2221 cgcctggtaa tgtggttgat ggcgcacagt gaaaaactgg aagatcttct ggaaggcaag
     2281 ccggttgtca ttattgaaga tggcgagctg gcctggtcga aactcaataa ctccaacatg
     2341 acggaatttg agttctttat ggagctacga ttgcgcggtg tagagcagtt ggggcaggta
     2401 cgtctggcga ttctcgaaac caacgggcaa atcagtgttt atttctttga agatgacaag
     2461 gtgaaaccgg gattacttat tttacccagt gactgtactc agcgttacaa agtggtgccg
     2521 gagtcggcgg actatgcctg catccgttgc agtgaaatca ttcatatgaa cgcgggggaa
     2581 aaacaattat gtccgcgctg tgcaaatcca gaattgacga aggcaagtcg ggctaaacgg
     2641 gtgacctgac agccaaaaca tcggtttttt gaaaataatc cgagagattc ttttgtgtga
     2701 cgcgtgcctc atttttgccc tcaacggttt tactcattgc gatgcgtgtc actgaatgat
     2761 aaaaccgata gccacaggaa taatgtattg cctgtggtcg caattgattg accgcgggtt
     2821 aatggcaacg caacgtggtg aggggaaatg gctcaaatct tcaattttag ttctggtccg
     2881 gcaatgctac cggcagaggt gcttaaacag gctcaacagg aactgcgcga ctggaacggt
     2941 cttggtacgt cggtgatgga agtgagtcac cgtggcaaag agttcattca ggttgcagag
     3001 gaagccgaga aggattttcg cgatcttctt aacgtcccct ccaactacaa ggtattgttc
     3061 tgccatggcg gtggtcgcgg tcaatttgct gctgtaccgc tgaatattct tggtgataaa
     3121 accactgcag attatgttga tgcgggttac tgggcagcaa gtgctatcaa agaagcgaaa
     3181 aaatactgca caccaaatgt gtttgatgcg aaagtgaccg ttgatggtct tcgcgcagtt
     3241 aagccaatga gtgaatggca actctctgat aatgctgctt atatgcatta ctgcccgaac
     3301 gaaaccattg acggtatcgc tatcgatgaa acaccagact tcggtaaaga cgtggtggtc
     3361 gccgctgact tctcttcaac cattctttcc cgtccgattg atgtcagccg ttatggtgta
     3421 atttacgctg gcgcgcagaa aaatatcggc ccggctggcc tgacaatcgt catcgttcgt
     3481 gaagatttgc tgggcaaagc gaatatcgcc tgcccatcga ttctggatta ctccatcctc
     3541 aacgataacg attccatgtt caacacgccg ccgacatttg cctggtactt atctggcctg
     3601 gtcttcaaat ggctgaaagc gaacggcggt gtggctgcaa tggataagat caatcagcaa
     3661 aaagcagaac tgctgtatgg ggtgattgat aacagcgatt tctaccgcaa tgatgtggcg
     3721 aaagctaacc gttcgcggat gaacgtgccg ttccagttgg cggatagcgc gcttgataaa
     3781 ttgttcctcg aagagtcttt cgctgcgggc ttacatgcgc tgaaaggtca ccgagtggtc
     3841 ggcggaatgc gtgcgtctat ttataacgcc atgccgctgg aaggtgttaa agcactgaca
     3901 gacttcatgg ttgagttcga acgtcgtcac ggctaatgtc gaaattttgc ataatcccca
     3961 cagccagcct gtggggtttt tatttctgtt gtagagagtt gagttcatgg aatccctaac
     4021 gttacaaccc atcgctcgtg tcgatggcac tattaatctg cccggttcca agagcgtttc
     4081 taaccgcgct ttattgctgg cggcattagc acacggcaaa acagtattaa ccaatctgct
     4141 ggatagcgat gacgtgcgcc atatgctgaa tgcattaaca gcgttagggg taagctatac
     4201 gctttcagcc gatcgtacgc gttgcgaaat tatcggtaac ggtggtccat tacacgcaga
     4261 aagtgcccgg gagttgttcc tcggtaacgc cggaacggca atgcgtccgc tggcggcagc
     4321 actttgcctg ggtagcaatg atattgtact gaccggtgaa ccgcgaatga aagaacgccc
     4381 gattggtcat ctggtggatg cgctgcgcca gggcggggcg aagatcactt acctggaaca
     4441 agaaaattat ccgccattgc gtttacaggg cggctttact ggcggcaacg ttgatgttga
     4501 tggctccgtt tccagccagt tcctcaccgc actgttaatg actgcgcctc ttgcgccgga
     4561 agatacggtg attcgtatta aaggcgatct ggtttctaaa ccttatatcg atatcaccct
     4621 caatctgatg aagacgtttg gtgttgaaat tgaaaatcag cactatcaac aatttgtcgt
     4681 aaaagggggg cagtcttacc agtctccggg cacttatttg gttgaaggcg atgcatcttc
     4741 ggcttcttat ttcctggcag cagcagcaat cagaggcggc actgtaaaag tgaccggcat
     4801 tggacgcaac agtatgcagg gtgatattcg ctttgctgat gttctggaaa aaatgggcgc
     4861 gaccatttgc tggggcgatg attatatttc ctgcacgcgt ggtgaactga acgccattga
     4921 tatggatatg aaccatattc ccgatgcggc gatgaccatt gccaccgcgg cgttatttgc
     4981 aaaaggcacc accacgctgc gcaatatcta taactggcgt gttaaagaga ccgatcgcct
     5041 gtttgcgatg gcaacagaac tgcgtaaagt cggtgcggaa gtcgaagagg ggcatgattt
     5101 cattcgcatt acgccaccgg aaaaactgaa atttgctgag atcgccactt ataatgatca
     5161 ccggatggcg atgtgtttct cgctggtcgc attatcagat acgcctgtga cgattcttga
     5221 tccaaaatgt accgctaaaa cttttccaga ctatttcgag caattggcgc ggataagtca
     5281 acccggctga atgaataacg ggcgataaca tagacaatcc tttcttttca aagacgtcgg
     5341 cacattgccg gcgttttttt tcggaccttg tggggtattt ttattaatgg tagcgttaat
     5401 tatcaatgta acttgttgat acataatgtt tatatataat taatcaacgg atgattcaca
     5461 tgaagaatac taaattattg ctggcgattg cgacctctgc agccttactg acaggatgtc
     5521 agaatacgca cggtattaat accgatttgg ctatcagctc aggtctgaat gcttataaag
     5581 ccgcaacatt aagcgatgct gatgctaaag caatcgccaa tcagggctgt gtcgaaatgg
     5641 acagcggcaa taaagtggca agcaaatcca gcaagtacgg gaaacgtctg gcaaaaatcg
     5701 ccaaagcatt gggtaacaat attaatggca cgccagtcaa ctataaggtt tatatgacca
     5761 gcgacgtcaa cgcatgggcg atggcgaacg gttgtgttcg tgtctacagt ggcctgatgg
     5821 acatgatgaa cgacaacgaa attgaaggcg ttctgggcca tgaactgggc cacgtcgcgt
     5881 tgggtcactc gctggctgaa atgaaagctt cttatgcgat cgtggccgca cgcgatgcca
     5941 tttcagctac cagcggtgtg gcttcccagc tttcccgttc acaattaggt gatatcgcag
     6001 aaggcgctat caatgctaaa tactcccgtg ataaagaatc cgaagcagat gatttctctt
     6061 ttgatctgtt gaagaaacgt ggcatcagta cccaggggct ggttggtagc tttgaaaaac
     6121 tggccagcct ggatggcggt cgcacccagt ccatgtttga ttctcaccca ccatcaacag
     6181 agcgtgcgca acacatccgt gatcgtatcg cctctggtaa gtaaatcatt gtcatctttc
     6241 gggctggtct tctgccagcc cgctataatt gcgcaataaa tccccatctg aatacagaca
     6301 aaactggttt ttgcacacaa cgttaacgat ttgtggcgtc ggcgcgtata atgcgcgcgg
     6361 ttatgttaac ggtacgcctg ttttaaggag ataaagatga cggcaattgc cccggttatt
     6421 accattgatg gcccaagcgg tgcagggaaa ggcaccttgt gtaaggctat ggcggaagcg
     6481 ttgcaatggc atctgctgga ttcgggtgca atttatcgcg tactggcact agcggcatta
     6541 catcaccatg ttgatgttgc atcggaagat gcgctggtgc cgctggcatc ccatctggat
     6601 gtacgttttg tgtcaaccaa cggtaatctg gaagtgatcc tcgaagggga agatgtcagc
     6661 ggcgaaattc gtactcagga ggtggcgaat gctgcttcac aagtcgcggc attcccacgc
     6721 gttcgtgaag cattattgcg tcgccaacgc gcgtttcgcg aattaccagg cctgattgcc
     6781 gatgggcgcg acatgggaac ggtggtattc cctgatgcgc cagtgaaaat tttccttgac
     6841 gcctcctcgg aagaacgtgc gcatcgccgc atgctacagt tgcaggagaa gggctttagt
     6901 gttaactttg agcgcctttt ggccgagatc aaagaacgcg acgaccgcga tcgtaaccga
     6961 gcggtagcgc cactggttcc ggcagccgat gctttagtgt tggattccac caccttaagc
     7021 attgagcaag tgattgaaaa agcgctacaa tacgcgcgcc agaaattggc tctcgcataa
     7081 gcgaccgaat ttgcagtacc cccgttgcaa tggaatgaca gcgggtatgt taaacaaccc
     7141 catccggcat ggagccaggt ggacgttaaa tataaacctg aagattaaac atgactgaat
     7201 cttttgctca actctttgaa gagtccttaa aagaaatcga aacccgcccg ggttctatcg
     7261 ttcgtggcgt tgttgttgct atcgacaaag acgtagtact ggttgacgct ggtctgaaat
     7321 ctgagtccgc catcccggct gagcagttca aaaacgccca gggcgagctg gaaatccagg
     7381 taggtgacga agttgacgtt gctctggacg cagtagaaga cggcttcggt gaaactctgc
     7441 tgtcccgtga gaaagctaaa cgtcacgaag cctggatcac gctggaaaaa gcttacgaag
     7501 atgctgaaac tgttaccggt gttatcaacg gcaaagttaa gggcggcttc actgttgagc
     7561 tgaacggtat tcgtgcgttc ctgccaggtt ctctggtaga cgttcgtccg gtgcgtgaca
     7621 ctctgcacct ggaaggcaaa gagcttgaat tcaaagtaat caagctggat cagaagcgca
     7681 acaacgttgt tgtttctcgt cgtgccgtta tcgaatccga aaacagcgca gagcgcgatc
     7741 agctgctgga aaacctgcag gaaggcatgg aagttaaagg tatcgttaag aacctcactg
     7801 actacggtgc attcgttgat ctgggcggcg ttgacggcct gctgcacatc actgacatgg
     7861 cctggaaacg cgttaagcat ccgagcgaaa tcgtcaacgt gggcgacgaa atcactgtta
     7921 aagtgctgaa gttcgaccgc gaacgtaccc gtgtatccct gggcctgaaa cagctgggcg
     7981 aagatccgtg ggtagctatc gctaaacgtt atccggaagg taccaaactg accggtcgcg
     8041 tgaccaacct gaccgactac ggctgcttcg ttgaaatcga agaaggcgtt gaaggcctgg
     8101 tacacgtttc cgaaatggat tggaccaaca aaaacatcca cccgtccaaa gttgttaacg
     8161 ttggcgatgt agtggaagtt atggttctgg atatcgacga agaacgtcgt cgtatctccc
     8221 tgggtctgaa acagtgcaaa gctaacccgt ggcagcagtt cgcggaaacc cacaacaagg
     8281 gcgaccgtgt tgaaggtaaa atcaagtcta tcactgactt cggtatcttc atcggcctgg
     8341 atggcggcat cgacggcctg gttcacctgt ctgacatctc ctggaacgtt gcaggcgaag
     8401 aagcagttcg tgaatacaaa aaaggcgacg aaatcgctgc agttgttctg caggttgacg
     8461 cagaacgtga acgtatctcc ctgggcgtta aacagctcgc agaagatccg ttcaacaact
     8521 gggttgctct gaacaagaaa ggcgctatcg taaccggtaa agtaactgca gttgacgcta
     8581 aaggcgcaac cgtagaactg gctgacggcg ttgaaggtta cctgcgtgct tctgaagcat
     8641 cccgtgaccg cgttgaagac gctaccctgg ttctgagcgt tggcgacgaa gttgaagcta
     8701 aattcaccgg cgttgatcgt aaaaaccgcg caatcagcct gtctgttcgt gcgaaagacg
     8761 aagctgacga gaaagatgca atcgcaactg ttaacaaaca ggaagatgca aacttctcca
     8821 acaacgcaat ggctgaagct ttcaaagcag ctaaaggcga gtaattctct gactcttcgg
     8881 gatttttatt ccgaagtttg ttgagtttac ttgacagatt gcaggtttcg tcctgtaatc
     8941 aagcactaag ggcggctacg gccgccctta atcaatgcag caacagcagc cgcttaattt
     9001 gcctttaagg aaccggagga atcatgacca agtcagaatt gatagaaaga cttgccaccc
     9061 agcaatcgca cattcccgcc aagacggttg aagatgcagt aaaagagatg ctggagcata
     9121 tggcctcgac tcttgcgcag ggcgagcgta ttgaaatccg cggtttcggc agtttctctt
     9181 tgcactaccg cgcaccacgt accggacgta atccgaagac tggcgataaa gtagaactgg
     9241 aaggaaaata cgttcctcac tttaaaccag gtaaagaact gcgcgatcgc gccaatattt
     9301 acggttaagt ttttactcaa acttgaacgc gagaaaagca cctgtcgggt gcttttttca
     9361 tttctctaat ctggaactgg aagctgcctc gcagagtttt gagcagtttt caccctatcg
     9421 ttaaattctt ctgaacgtgc ctcggggaac gcaaaactcc caccagacaa ccgttcaaca
     9481 aagttgcaca ctttctataa acagggaggg tgcatgaaaa taacgacagt cggcgtatgc
     9541 ataatttgcg gaatttttcc gttgctgatt ttgccccaat tgccagggat agtaaccctt
     9601 gcgtttctga ctttcttcgc ctgtgtactg gcatttatcc ctgttaaaac cgtccgttat
     9661 atcgcgctga cgttgctgtt tttcgtttgg ggcatattag cagcaaagca aattttgtgg
     9721 gcaggagaaa ccttaactgg cgcgacgcag gatgcaattg ttgagatcat tgctactgac
     9781 ggcatgacca ctcattacgg tcaaattact catctacaag gtcgacgtat attccctgcg
     9841 ccaggcctcg tactgtatgg cgaatatctt ccgcaagcgg tttgtgccgg acaagtatgg
     9901 tcaatgaaac tcaaagttcg tgcagttcat ggtcaactta atgatggcgg ctttgatagc
     9961 cagcgttatg ccattgccca gcatcaaccg ctcaccggcc gttttctgca ggcaagtgtc
    10021 attgaaccga attgtagcct gcgtgcacag tatctggcgt cactacaaac aacgctgcaa
    10081 ccctatatgt ggaacgcggt tattcttgga ttaggtatgg gggaacggtt atccgtcccc
    10141 aaagaaatca aaaatatcat gcgtgatact ggaacggcgc atttaatggc gatatcagga
    10201 ttgcacatcg cttttgcggc gttgctggct gcaggactca ttcgcggtgg gcaagttttt
    10261 ctgcctgggt gctggatcca ctggcaaatg ccattaattg gtggaatctg ctgtgctgct
    10321 ttttatgcct ggcttacggg aatgcaacct cctgcattgc gtaccgtggt ggcgcttgcc
    10381 atttggggaa tgcttaagtt aagtggacga caatggagtg gctgggatgt atggatatgt
    10441 tgtctggcgg caattttgct gatggatcct gttgccattc tctcgcaaag tttatggctc
    10501 tctgccgctg cggtcgcggc actgattttt tggtatcagt ggtttccctg tccggagtgg
    10561 cagctgccgc cggtattgcg cgcggttgtt tccctcatcc atctgcaact gggaatcacc
    10621 ctcctgctta tgcccgtgca aattgtcata tttcatggca ttagtctgac ctcgtttatt
    10681 gcaaatcttt ttgcaattcc cctggtgaca tttatcacgg ttccgttgat tctcgccgcg
    10741 atggttgtgc atttaagcgg gccgtttatt ctggaacaag ggttatggtt tcttgccgac
    10801 cggtctttgg ctttactttt ctgggggtta aagagtttgc cggaagggtg gatcaacatt
    10861 gctgaacgtt ggcaatggct aacattttcc ccatggttct tactggtggt atggcgatta
    10921 aacgcctggc gaacgttgcc agcaatgtgt gtggctgtag gcttgctgat gtgcttgccg
    10981 ctgtggcaaa aacctcgacc tgacgagtgg caagtgtaca tgcttgatgt cgggcaaggg
    11041 ctggcaatgg tcattgcgcg aaacggcaaa gcgattctct atgacacggg actggcctgg
    11101 cccgaagggg atagtgggca acaactgatt atcccctggc tccactggca taatcttgaa
    11161 ccggaaggcg ttattttgag tcatgaacat ctggatcacc ggggagggct ggactcaata
    11221 ttgcacacat ggccgatgtt atggatccga agtccgttaa actgggaaca tcatcagccc
    11281 tgtgtgcgtg gcgaagcgtg gcaatggcaa ggattgcgtt tcagcgcgca ctggccttta
    11341 caaggtagca acgataaagg aaataaccat tcctgtgtgg ttaagattga tgacgggacg
    11401 aatagcattc ttctaaccgg tgatattgaa gcccccgctg aacaaaagat gctaagccgt
    11461 tactggcagc aaatgcaggc aacattactt caggtacctc accatggcag taatacctca
    11521 tcatcgttgc cattaattca gcgagtgaat ggaaaagtgg cactcgcatc ggcatcgcgc
    11581 tataacgcat ggcgactgcc ctcaagcaaa gttaaacatc gctatcaaca acagggttat
    11641 aagtggcttg atactccaca tcagggtcaa ataacggtca atttttcagc gcaaggctgg
    11701 cggattagca gcctcagaga gcaaatttta cctcgttggt atcatcagtg gtttggcgtg
    11761 ccagtggata acgggtagaa tatgcggcta tttcaacaaa tgctggtttt ttgaatgcat
    11821 aacgacaaag atctctctac gtggcagacg ttccgccgac tgtggccaac cattgcgcct
    11881 tttaaagcgg gtctgatcgt ggcgggcgta gcgttaatcc tcaacgcagc cagcgatacc
    11941 ttcatgttat cgctccttaa gccacttctt gatgatggct ttggtaaaac agatcgctcc
    12001 gtgctgatgt ggatgccgct ggtggtgatc gggctgatga ttttacgtgg tatcaccagc
    12061 tatatctcca gctactgtat ctcctgggta tcaggaaagg tggtaatgac tatgcgtcgc
    12121 cgcctgtttg gtcacatgat gggaatgccg gtttcattct ttgacaaaca gtcaacgggt
    12181 acgctgttgt cacgtatcac ctacgattcc gaacaggtcg cttcttcttc ttccggcgca
    12241 ctgattactg ttgtgcgtga aggtgcgtcg atcatcggcc tgttcatcat gatgttctat
    12301 tacagttggc aactgtcgat cattttgatt gtgctggcac cgattgtttc gattgcgatt
    12361 cgcgttgtat cgaagcgttt tcgcaacatc agtaaaaaca tgcagaacac catggggcag
    12421 gtgaccacca gcgccgaaca aatgctgaaa ggccataaag aagtattgat tttcggtggt
    12481 caggaagtgg aaacgaaacg cttcgataaa gtcagtaacc gaatgcgtct tcaggggatg
    12541 aaaatggttt cagcctcttc tatctctgat ccgatcattc agctgatcgc ctctttggcg
    12601 ctggcgtttg ttctgtatgc agcaagcttc ccaagtgtca tggatagcct gactgccggt
    12661 acgattaccg ttgttttctc ttcaatgatt gcacttatgc gtccgctgaa atcgctgacc
    12721 aacgttaacg cccagttcca gcgtggtatg gcggcttgtc agacgctgtt taccattctg
    12781 gacagtgagc aggagaaaga cgaaggtaag cgcgtgatcg agcgtgcaac tggcgacgtg
    12841 gaatttcgca atgtcacctt tacttatcca ggacgtgacg tgcccgcatt gcgtaacatc
    12901 aacctgaaaa ttccggcagg gaagacggtt gctctggttg gacgatctgg ttcaggtaaa
    12961 tcaaccatcg ccagcctaat cacacgtttt tacgatattg atgaaggcga aatcctgatg
    13021 gatggtcacg atctgcgcga gtataccctg gcgtcgttac gtaaccaggt tgctctggtg
    13081 tcgcagaatg tccatctgtt taacgatacg gttgccaaca acattgctta cgcaaggact
    13141 gaacagtaca gccgtgagca aattgaagaa gctgcgcgta tggcctacgc catggacttc
    13201 atcaataaga tggataacgg tctcgataca gtcattggtg aaaacggcgt gttgctctct
    13261 ggcggtcagc gtcagcgtat tgctatcgct cgagccttgt tgcgtgatag cccgatactg
    13321 attctggacg aagctacctc ggcgttggat accgaatccg aacgtgcgat tcaggcggca
    13381 ctggatgagt tgcagaaaaa ccgtacctct ctggtgattg cccaccgctt gtctaccatt
    13441 gaaaaggcag acgaaatcgt ggttgtcgag gatggtgtca ttgtggaacg cggtacgcat
    13501 aacgatttgc ttgagcaccg tggcgtttac gcgcaacttc acaaaatgca gtttggccaa
    13561 tgatcgaaaa aatctggtct ggtgaatccc ctttatggcg gctattgctg ccactctcct
    13621 ggttgtatgg cctggtgagt ggcgcgatcc gtctttgcta taaactaaaa ctgaagcgcg
    13681 cctggcgtgc ccccataccg gttgtcgtgg ttggtaatct caccgcaggc ggcaacggaa
    13741 aaaccccggt cgttgtctgg ctggtggaac agctgcaaca gcgcggtatt cgcgtggggg
    13801 ttgtatcgcg gggatatggt ggtaaggctg aatcttatcc gctgttattg tcggcagata
    13861 ccaccacagc acaggcgggt gatgaacctg tgttgattta tcaacgcact ggcgcacctg
    13921 ttgcggtttc tcccgtgcgt tctgatgcga taaaagccat tctggcgcaa caccctgatg
    13981 tgcagatcat cgtaaccgac gacggtttac agcattaccg tctggcgcgt gatgtggaaa
    14041 ttgtcgttat tgatggtgtg cgtcgctttg gcaatggctg gtggttgccg gcggggccaa
    14101 tgcgtgagcg agcggggcgc ttaaagtcgg ttgatgcggt aatcgtcaac ggtggtgtcc
    14161 cccgcagcgg tgaaatcccc atgcatctgc tgccgggtca ggcggtgaat ttacgtaccg
    14221 gtacgcgttg tgacgttgct cagcttgaac atgtggtggc gatggctggg attggtcatc
    14281 cgccgcgctt ttttgccacg ctgaagatgt gtggcgtaca accggaaaaa tgtgtaccgc
    14341 tggccgatca tcagtctttg aatcatgcgg atgtcagcgc gttggtaagc gccgggcaaa
    14401 cgctggtaat gactgaaaaa gatgcggtga aatgccgggc ctttgcagaa gaaaattggt
    14461 ggtatttgcc tgtagacgca cagctttcag gtgatgaacc agcgaaactg cttgcgcaac
    14521 taacctcgct ggcttctggc aactagttac gccgcggcag cgttcgattg atggagtcat
    14581 gaatgtcgct gccgcacctc tctcttgctg atgcgcgtaa tcttcacctc gccgcgcaag
    14641 gcctgttaaa caaaccccac cgtcgagcgt cgttggagga tattccggca acgatctccc
    14701 gcatgtcctt gctgcaaatc gataccatta atattgttgc ccgtagccca tatctggtgc
    14761 ttttcagtcg tctgggaaat tatcctgccc agtggctgga tgagtctctg gcgcgtggcg
    14821 aattaatgga atactgggcg catgaagcct gctttatgcc gcgtagcgat tttcgtctta
    14881 ttcgccaccg catgctggca cctgaaaaaa tgggctggaa atacaaagac gcctggatgc
    14941 aggaacatgc ggcggaaatt gcacagttaa ttcagcatat tcatgataag gggccggtac
    15001 gttcagccga tttcgagcat tctcgtaaag gtgcaagcgg ctggtgggaa tggaagccgc
    15061 ataaacggca tctggaaggt ttatttactg ccggaaaggt gatggtgatt gaacggcgca
    15121 acttccagcg cgtttatgat ttaacccact gtgtcatgcc tgactgggat gatgagcgcg
    15181 atctcgtttc gcaaacagaa gcagaaatca tcatgctgga taacagtgcg cgtagcctgg
    15241 gcatattccg cgaacagtgg ctggcagatt actatcggct gaaacgtccg gcactggcgg
    15301 cttggcgcga agcgagggct gaacagcagc aaatcattgc tgtgcatgtt gaaaaattgg
    15361 gcaatctttg gctgcatgct gatttgctgc cgctactcga gcgtgcgctc gccggaaagc
    15421 tcactgcaac gcacagcgcg gtactttcgc cttttgatcc tgttgtctgg gatcgcaaac
    15481 gcgcggagca gttttttgat tttagctacc ggcgggagtg ctatatccca gcgccgaaac
    15541 gccagtatgg ctattttgtt ctgccgttat tacatcgtgg gcaattagtt gggcgaatgg
    15601 atgccaaaat gcatcgccag acaggcatcc ttgaagttat ctctctgtgg ttacaggaag
    15661 gcattaaacc aacgacaacg ctgcaaaaag ggttacgtca ggcgattact gatttcgcta
    15721 actggcagca ggcaacgcgg gtgacattag gacgctgccc gcaaggcctc tttactgatt
    15781 gccgcgccgg ctgggaaata gaccccgtcg cataaatgat tatgataaga tttatgcatt
    15841 cataacccga tccatctgga ggaactatgg atcatcgtct gcttgaaatc attgcctgcc
    15901 cggtttgcaa cggaaaactt tggtataacc aggaaaaaca agagcttatt tgcaaactgg
    15961 acaacctggc tttcccgctg cgcgatggca ttccagtgtt gctggaaacg gaagcccgcg
    16021 tgctgactgc tgatgagagt aaatcatgag ttttgtggtc attattcccg cgcgctacgc
    16081 gtccacgcgt ctgcccggta aaccattggt tgatattaac ggcaaaccca tgattgttca
    16141 tgttcttgaa cgcgcgcgcg aatcaggtgc cgagcgcatc atcgtggcaa ccgatcatga
    16201 ggatgttgcc cgcgctgttg aagccgctgg cggtgaagta tgtatgacgc gcgccgatca
    16261 tcagtcagga acagaacgtc tggcggaagt tgtcgaaaaa tgcgcattca gcgacgacac
    16321 ggtgatcgtt aatgtgcagg gggatgaacc gatgatccct gcgacaatca ttcgtcaggt
    16381 tgctgataac ctcgctcagc gtcaggtggg tatggcgact ctggcggtgc caatccacaa
    16441 tgcggaagaa gcgtttaacc cgaatgcggt gaaagtggtt ctcgacgccg aagggtacgc
    16501 actgtacttc tctcgcgcca ccattccgtg ggatcgtgat cgttttgcaa aagaccttga
    16561 aaccgtaggc gataacttcc tgcgtcatct tggtatttat ggctaccgag caggctttat
    16621 ccgtcgttat gttacctggc agccaagtcc gttagaacat atcgaaatgt tagagcagct
    16681 tcgtgttctg tggtacggcg aaaaaatcca cgttgctgtc gcccaggaag ttcctggcac
    16741 aggtgtggat acccctgaag atctcaagcg cgttcgcgcc gaaatgcgct aatttcactt
    16801 cacgacactc cagccaattt tgggaggagt gtcgtaccgt tacgattttc ctcaattttt
    16861 cttttcaaca attgatctca ttcaggtgac atcttttata ttggcgctca ttatgaaagc
    16921 agtagctttt atgagggtaa tctgaatgga acagctgcgt gccgaattaa gccatttact
    16981 gggcgaaaaa ctcagtcgta ttgagtgcgt caatgaaaaa gcggatacgg cgttgtgggc
    17041 tttgtatgac agccagggaa acccaatgcc gttaatggca agaagcttta gtgcgcccgg
    17101 aaaagcccga caactggcat ggaaaaccac catgctggca agaagtggga ctgtccgtat
    17161 gccgactatt tatggtgtga tgacgcatga agaacacccc ggccccgatg tcctgctact
    17221 ggagcgaatg cgtggtgttt cggtggaggc accagcccga acaccagaac gctgggaaca
    17281 actcaaagat caaatcgttg aagccttact ggcctggcac cgtcaggaca gtcgtggttg
    17341 cgtcggcgcg gtcgacaata ctcaggaaaa tttctggccc tcatggtacc ggcaacatgt
    17401 tgaagtgcta tggaccacgc tcaatcagtt caataacacc ggtctaacga tgcaggataa
    17461 gcggatcctg tttcgcactc gcgaatgtct cccggcatta tttgaaggct ttaacgacaa
    17521 ttgtgtactg attcacggta acttctgttt acgtagcatg ctgaaagatt cgcgcagcga
    17581 tcagttactg gcgatggtcg gaccgggact aatgctttgg gcaccgcgag aatacgaact
    17641 gttccgacta atggataatt ctctggcgga agatttgctc tggagttatc tgcaacgcgc
    17701 gccagtggcg gagtcgttca tctggcggcg ttggttgtat gtgttatggg atgaagttgc
    17761 gcaactggtc aacaccggac gatttagtcg gcgtaacttc gatctggcat caaaatcact
    17821 cttgccgtgg ctcgcctgac gaacctttca gccactgcca gatccggcca agcgtttcat
    17881 agccaacgtg gtcgctatgc atcaaccaaa ccggagaagg gattgcccgt tcccacggat
    17941 taagcggcga gtcgatggcc aactgattcg ccggggctgg tagcggattt aacccttcct
    18001 gctgaaaaaa gatcatcgcc cgcggcagat gtgatgcgga ggtcaccagc aggaaaggtg
    18061 cgtcaccaat cgcctgtttc actgctgcag cttcttcttc ggtatctttt ggcaaatcca
    18121 gggtgataat ttgctcgcgc ggcacgccca gcgattgcgc aactctggca cctacttcgg
    18181 ctgtgctcac cgtattggtt ttagctacgc ctcccgtaaa gatcagtttt gatcccggat
    18241 tttcgcgcca taagcgaata ccttcattca ggcgtggcag actgttattg attaaattag
    18301 agctcggcgc ccactgcggg ttccaggtat aaccacctcc gagcaccacg atatagtcca
    18361 ctttttgcga attattccat gtcggataag tgctttcgat gggacgcaac aggcggtcgg
    18421 ctatcggttg caaactcaat aacaagagcg ccagccaccc aatgctaata aaaatcttcc
    18481 cggttttttg aaaacggcta aaccacagca gcgccaggcc agcgccaata atcaacaaca
    18541 tcagcggaag tggcagcaac atattgccaa tcactttttt cagtgtaaaa agcatccttt
    18601 ctggttcctt ttttgaccat atagcaaggg atcgccagcg atattacacc agacgggttc
    18661 attctcgccg tggctgtgac aaaatagcgt ctttatcaga aaacgtctta gccaaccggt
    18721 ggagaaagca atgcaggatc gcaattttga tgatattgcg gaaaagtttt cccgtaacat
    18781 ttacggcacc accaaagggc agcttcgaca ggctattctg tggcaggatc tcgatcgcgt
    18841 gctggcggaa atgggcccgc aaaaactgcg tgtgctggat gctggcggtg gagaagggca
    18901 gaccgcaatc aaaatggccg agcgcgggca tcaggtcatt ttatgcgatc tttctgcgca
    18961 gatgatcgac cgcgcaaaac aggcggcaga agcaaaaggt gtgagcgaca acatgcaatt
    19021 tatacattgc gccgctcagg atgttgcttc gcatttggaa acgcccgttg atctgatatt
    19081 gttccatgcg gtgctcgagt gggtggccga tccccgcagc gtattacaga ccctgtggtc
    19141 agtgttgcgt ccaggcggcg tgttgtcgtt aatgttctac aatgcgcatg gtttgttgat
    19201 gcataacatg gtcgccggga attttgatta cgtgcaggcg ggaatgccga aaaagaaaaa
    19261 acggacgctt tcgccagatt atccacgcga cccggcgcag gtttatctgt ggctggaaga
    19321 agctggttgg caaattctgg gtaagacagg cgttcgcgtg tttcatgatt atctgcgcga
    19381 gaagcaccag cagcgcgact gctatgaagc attacttgaa ttagaaacgc gttattgccg
    19441 tcaggaaccg tatattaccc tggggcgtta tattcatgtc accgcgcgca aaccgcagag
    19501 caaggataaa gtatgagtga attttcccag acagtccccg aactggttgc ctgggccaga
    19561 aaaaatgact tctccatctc gctgccggta gaccgactct cttttctgct ggcggttgcc
    19621 acgctgaacg gcgagcgtct ggatggtgag atgagtgaag gcgagctggt ggatgcattc
    19681 cgccatgtga gtgatgcgtt tgagcaaacc agcgaaacca tcggcgtgcg cgccaacaac
    19741 gcgatcaacg acatggtgcg tcaacgtctg ctgaaccgct ttaccagcga gcaggcggaa
    19801 gggaacgcaa tttaccgtct gacgccgctc ggcatcggca tcactgacta ttacattcgt
    19861 cagcgcgagt tttctacgct gcgcctttct atgcaattgt cgattgtggc gggtgagctc
    19921 aaacgcgcag cagatgccgc cgaagagggc ggtgatgaat ttcactggca ccgtaatgtt
    19981 tatgcgccac tgaaatattc ggtagcagaa attttcgaca gtatcgacct gacgcaacgt
    20041 ctgatggatg aacagcagca gcaggtgaag gatgatatcg cccagttgtt gaacaaagac
    20101 tggcgggcgg cgatttccag ctgtgaattg ttgctttcag aaacttccgg aacgctgcgt
    20161 gaattgcagg atacgctgga agcggcaggc gataaattgc aggccaacct gttacgcatt
    20221 caggatgcga cgatgaccca tgacgacctg cattttgtcg atcgtctggt gttcgatctt
    20281 cagagcaaac tcgaccgtat tatcagttgg ggccagcaat ccatcgactt gtggattggc
    20341 tacgaccgcc acgtacacaa atttattcgt actgcgatcg atatggataa aaaccgcgtc
    20401 tttgctcagc ggttacgtca gtcggtacaa acctattttg atgagccgtg ggcgctaact
    20461 tatgccaatg ccgatcgtct gctggatatg cgtgacgaag agatggtact gcgcgatgaa
    20521 gaagtgactg gggaacttcc tgaggatctg gagtacgaag agtttaacga gatccgcgaa
    20581 cagctggcgg cgatcatcga agaacaactt gccgtgtaca aaaccagaca agtgccgttg
    20641 gatcttggtc tggtggtacg cgaatatctg tcacagtatc cgcgtgcacg tcactttgac
    20701 gttgcgcgca ttgttattga tcaggcggta cgtcttggcg tagcgcaagc agatttcacc
    20761 ggactgccag cgaaatggca gccgattaat gattacggag ccaaggtaca ggcgcatgtc
    20821 atcgacaaat attgaacaag tgatgccggt taagctggcg caggcgctgg cgaatccgtt
    20881 atttccggcg ctggatagcg ccttacgttc aggccgtcat attggcctcg acgaactgga
    20941 taatcatgca ttcctgatgg attttcagga atatctggaa gagttttacg cgcgttataa
    21001 cgttgagctt attcgtgcac cggaagggtt cttctatcta cgcccacgtt ccaccacgct
    21061 gatccctcgt tccgtcttgt cggaactgga tatgatggtc gggaaaatcc tctgttatct
    21121 ctatctcagc ccggaacggc tggcgaatga ggggattttt actcaacagg aactgtacga
    21181 cgaactgctc accctggccg atgaagcgaa actgctgaaa ctggtgaaca accgttcaac
    21241 cggttcagac gttgaccgtc agaagttgca ggaaaaagta cgttcttcgc tcaaccgtct
    21301 gcgtcgttta ggcatggtgt ggtttatggg ccacgacagc agcaagttcc gcattaccga
    21361 atcggtgttc cgcttcggag ccgatgtgcg tgctggcgac gatccccgtg aagcacagcg
    21421 tcgcctgatt cgtgatggtg aagcaatgcc gattgaaaac catctgcaac tcaacgatga
    21481 aaccgaagag agtcagccag atagcggaga ggaagaataa tgattgaacg cggtaaattt
    21541 cgctcactga cgctgattaa ctggaacggc ttttttgccc gaacttttga ccttgacgag
    21601 ctggtcacga cgctttctgg cggtaacggg gcgggtaaat ccaccaccat ggcggcgttc
    21661 gttacggcgc ttatccccga cctgaccctg ctgcatttcc gtaacactac ggaagccggg
    21721 gccaccagcg gttcgcgcga taaaggtctg cacggtaagc tgaaagcggg tgtctgttat
    21781 tcgatgctcg acaccattaa ctcgcgccac cagcgtgtag tggtcggtgt gcgtctgcaa
    21841 caggttgccg gacgcgatcg taaagtggat atcaaaccat ttgccattca gggcctgccg
    21901 atgtcggtgc agccgacaca actggtgacc gaaactctga acgaacgcca ggcgcgcgtg
    21961 ctgccgctta acgagctgaa agacaagctc gaggcgatgg aaggcgtgca gtttaaacag
    22021 ttcaactcca ttaccgatta ccactcgctg atgttcgatc tgggcatcat cgcgcgtcgt
    22081 ctgcgctcag cgtctgaccg tagtaaattc tatcgtctga tcgaagcctc gctgtatggc
    22141 gggatctcca gcgccattac tcgttctctg cgcgactacc tgttgccgga aaacagtggg
    22201 gtgcggaaag cgttccagga catggaagcg gcgctgcgtg aaaaccgcat gacgctggaa
    22261 gcgattcgtg tcacccagtc ggaccgtgac ctgtttaagc atctgatcag cgaagccacc
    22321 aactacgtgg cggcggacta catgcgtcac gccaacgagc gccgtgttca tctcgataag
    22381 gccctggagt ttcgtcgcga gctacatact tcgcgtcagc aactggcggc tgagcagtac
    22441 aaacacgtcg acatggcgcg agagctggct gagcacaacg gtgccgaagg cgatctggaa
    22501 gcggattatc aggcggccag cgatcacctg aacctggtgc aaaccgcact gcgtcagcag
    22561 gagaagatcg aacgctacga agcggatctc gatgagctgc agatccgtct ggaagagcaa
    22621 aacgaagtgg tggcagaagc catcgaacgc caggaagaga acgaggctcg tgcggaagct
    22681 gccgaactgg aagtggacga gctgaaaagc cagcttgctg actaccagca ggcgctggac
    22741 gtccagcaaa cgcgcgcgat ccagtataac caggcgattg ctgcgcttaa tcgtgccaaa
    22801 gaactgtgcc atctgccgga cttaaccgcc gacagcgctg ccgaatggct ggaaaccttc
    22861 caggcgaaag agctggaagc gaccgaaaaa atgctctctc ttgagcagaa aatgagcatg
    22921 gcgcaaaccg cgcacagcca gtttgagcag gcttatcagc tggtggtggc aatcaacggc
    22981 ccgctggcgc gtaacgaggc gtgggatgtc gctcgcgaac tattgcgcga aggggtcgat
    23041 cagcgtcacc tggcagagca ggttcagccg ttgcggatgc gcttaagcga actggaacag
    23101 cgtctgcgtg agcagcaaga agctgagcgt ctgctggcag atttctgcaa acgtcagggc
    23161 aagaattttg atattgatga actggaagct ctgcatcagg agctggaagc acgcattgcc
    23221 tctctttccg atagcgtctc taacgcccga gaagagcgga tggcactgcg ccaggagcag
    23281 gaacagctgc agtctcgcat tcagagtttg atgcagcgtg cgccggtttg gctggcagcg
    23341 caaaacagtc tcaaccagtt gagcgaacag tgcggcgaag agttcacctc cagccaggat
    23401 gtcaccgaat ttctgcaaca gttgctggag cgtgagcgcg aggcaattgt tgaacgcgat
    23461 gaagtaggcg cgcgcaaaaa cgccgtcgat gaagagatcg aacgtttaag ccagccaggt
    23521 ggttctgaag atcagcgtct gaacgcgctg gcggagcgtt ttggtggtgt gctgctgtca
    23581 gaaatttatg acgacgttag cctggaagat gcgccgtact tctcagcgct gtatggcccg
    23641 tcacgccacg ccatcgtggt gccagatctg tcacaggtaa ccgaacacct ggaaggcttg
    23701 accgattgcc cggaagatct ctatctgatc gaaggggatc cgcagtcatt cgatgacagc
    23761 gtgttcagcg ttgatgagct ggaaaaagcg gtggtggtga aaatcgccga tcgtcagtgg
    23821 cgttattcac gtttcccgga agtgccgctg tttggtcgtg ctgcgcgtga aagccgtatt
    23881 gaaagcctcc atgccgagcg ggaagtgctt tccgaacgct tcgccacgct ctcctttgat
    23941 gtacagaaaa cccagcgtct gcatcaggcg ttcagccgct ttatcggcag tcatctggcg
    24001 gtggcgtttg agtctgaccc ggaagcagaa atccgtcaac tgaacagccg tcgcgtcgaa
    24061 ctggaacggg cgttgagtaa tcatgaaaat gataaccagc agcagcgtat tcagtttgag
    24121 caggcgaaag agggcgttac ggcgctgaac cgcattctgc cgcgtctcaa cctgttggct
    24181 gatgacagcc tggcggatcg cgtcgatgaa atccgcgaac gtctggatga agcccaggaa
    24241 gccgcgcgtt ttgttcagca gtttggcaat caactggcga aactggaacc gatcgtttcg
    24301 gtattgcaga gcgacccgga acagttcgaa cagttaaaag aagattacgc gtactctcag
    24361 cagatgcagc gcgatgcccg tcagcaggcg tttgccctga cggaagtggt gcagcgtcgt
    24421 gcgcacttta gctactctga ctcggcagaa atgcttagcg gtaacagcga tctcaacgaa
    24481 aaactgcgtg aacgtctgga acaggcggaa gcggagcgta cccgcgctcg tgaagcgttg
    24541 cgcggacacg cagcgcagtt gaatcagtac aaccaggtgc tggcttcgct gaaaagttcg
    24601 tatgacacca aaaaagagct acttaacgat ctgcaacgtg aattgcagga tatcggcgtg
    24661 cgtgctgata gcggggcaga agagcgggca cgtattcgcc gtgacgagtt gcacgcgcaa
    24721 ctgagcaata accgttcacg acgcaatcaa ctggaaaaag cgcttacatt ctgcgaagcg
    24781 gagatggaca acctgacccg taaactgcgc aagctggagc gggattactt tgagatgcgc
    24841 gagcaggtag tgaccgccaa agcgggctgg tgtgcggtga tgcgcatggt gaaagataat
    24901 ggcgttgagc gtcgcttaca ccgtcgtgag ctggcttatc tctccgctga tgatttgcgt
    24961 tccatgtcgg ataaggcgtt aggtgcgctg cgtctggcgg tggcagataa cgaacatctg
    25021 cgcgacgtgc tgcggatgtc ggaagatcca aaacgtccgg aacgtaaaat tcagttcttc
    25081 gtggcggttt atcagcatct gcgtgaacgt attcgtcagg atattattcg taccgatgat
    25141 ccggtggaag ctatcgaaca gatggagatc gagcttagcc gtctgaccga agaattaacc
    25201 tcccgtgaac agaaactggc gatcagttcc cgcagcgtgg cgaacatcat tcgcaaaacc
    25261 attcagcgcg agcagaaccg tatccgtatg ctcaaccagg ggttgcagaa cgtatcgttt
    25321 ggccaggtga acagcgtacg tctgaacgtg aacgtacgtg aaacgcacgc catgctgctg
    25381 gatgtgctct ctgaacagca cgagcagcat caggatctgt ttaacagcaa ccgcctgacc
    25441 ttctcagaag cgctggcgaa actgtatcaa cgtcttaacc cgcagattga tatggggcag
    25501 cgcacgccgc agaccatcgg tgaagaactg ctggattacc gcaactatct ggaaatggaa
    25561 gttgaggtta accgtggttc cgatggctgg ttgcgtgcag agtctggtgc attgtcgacc
    25621 ggtgaggcga ttggtaccgg tatgtcgatt ctggtgatcg tggtacaaag ctgggaagat
    25681 gaatctcgcc gcctgcgcgg taaagatatc tctccttgcc gcctgctgtt cctcgatgaa
    25741 gcagcgcgac tggatgcccg ttctatcgcc acgctgtttg aattgtgtga acgtttgcaa
    25801 atgcagctca tcatcgcagc gccggaaaat atcagcccgg agaaaggcac cacctataaa
    25861 ctggtgcgta aagtcttcca gaataccgaa cacgttcacg tcgtcggcct gcgaggattt
    25921 gcgccgcaac tccctgaaac gcttccagga agtgacgaag cgccttctca ggcgagttaa
    25981 aattaaggcg gcagtaatgc cgccttttct ttttccgaaa actccgtttc tgcactaaaa
    26041 aagtggcaca ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg
    26101 tatcggcgtt tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt
    26161 cgtgagtatt ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg
    26221 ataaaaacag ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa
    26281 tcagtttgtg cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg
    26341 aagtaatccc tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg
    26401 cgcaagccac ggcaataatg gcggggatcc agccattgcc tgaaggtgtg gctgaaaaag
    26461 cccgcacgca aatcgaatct caattacccg caggttataa gccggtttat cttaaccagc
    26521 ttcaattgtt gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta
    26581 aagccttcca gcaacagctg gcagaggtgg cgattgccgg tttccagccg cagtttaata
    26641 aatgggtaga gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtggtgctct
    26701 ctgatgcgat gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct
    26761 ggctatatag cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat
    26821 ggcagctggc gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc
    26881 atccgcaata tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt
    26941 ggccccaact gaccggcaaa gcaacgttgc gcccaggaca gtggagtaac gacgtaccgg
    27001 cgttgcgcga aatattgcaa cgtacaggca tgttggacgg gggaccgaaa attacgctac
    27061 ctggcgatga cacgccaact gacgcggtag tcagcccatc cgctgttact attgaaacag
    27121 cagaaactaa gccgatggac aagcaaacta cgtctcgtag taaacctgcg cctgtcgttc
    27181 gtgccgccta cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat
    27241 tgggggcaga tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc
    27301 agcgtgctgg cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt
    27361 ctaccgggat catggttaac attccggcct attcgctggt ctactatcag aacggcaatc
    27421 aggtgctgga ttcgcgagtc attgtcggtc gccccgatcg caaaacgcca atgatgagca
    27481 gtgcccttaa caacgtagtg gtaaacccgc cgtggaacgt cccaccaact ctggcacgca
    27541 aagatattct gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg
    27601 tgatgcgcgg ctggaacaac agagaagcga ttgacccatg gcaggttgac tggtctacaa
    27661 tcacggcctc gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg
    27721 ggcgctataa attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc
    27781 acaatctgtt caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag
    27841 cttccgatct ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg
    27901 atgcgctgaa gcagggcgat acgcgttacg tcaatattcg gcagtcgatt ccggtgaatc
    27961 tctactatct gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt
    28021 acaattatga tctgcctgcg cgatccagct cgcaaattgt atcgaaagcg gaacaattaa
    28081 tcaggtaaat gaagcagttc ggggaaatca gttgtcgtaa taattagcat gatcgggggc
    28141 gattctctgc agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc
    28201 ggttaaggtg cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact
    28261 tgattatcat ggacaaattc gacgctaatc gccgcaaact gctggcgctt ggtggcgttg
    28321 cactcggtgc cgccatcctg ccgaccccgg cgtttgcaac actctctacc ccacgcccgc
    28381 gcattttgac actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg
    28441 gcagaggcta tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg
    28501 cgaacaaaat aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt
    28561 tgttaggcac tcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca
    28621 atgaactacg cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg
    28681 cgatggattt ccacattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta
    28741 tgcgcgcagg tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc
    28801 cagcacggca ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat
    28861 tccggtcacc gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgtctggc
    28921 cgcactggtc gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgccagcgg
    28981 cctgacactg atgcagatcc tgctgactca tggtcatctg gaccacgttg gcgcagcggc
    29041 ggaactggcg caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg
    29101 gctgcaaggc ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac
    29161 gccagatcgt tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt
    29221 gttacattgc cctgggcata cgccgggtca tgtcgtgttt ttagatgatc gggcaaagct
    29281 gctgatttct ggcgacgtta ttttcaaagg tggagtaggg cgcagtgact tcccgcgtgg
    29341 cgatcataat caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt
    29401 gacatttatt ccgggtcacg gaccattatc cacacttggt tatgagcgcc tgcataatcc
    29461 cttcctgcaa gacgaaatgc ccgtctggta aaaaggcgca taaaaaagcc cgctttcaag
    29521 cgggcttcat cggttttaac gcttacagca ctgccacaat cgcttcgcac agcggcgcca
    29581 tgttatctgg tgtcatcccg gccacgttta cgcgaccaga agcaacagca tatacgccaa
    29641 actcttcgcg cagacgcagc acttgttctt ttgtcaggcc actgaaggag aacatgccgt
    29701 tctgtttgat gataaagctg aagtcgcgat ttgcgccttt ttcctgcagc gtattgacga
    29761 acaactgacg catacgctga atacgctggc gcatatcagt cagttcttgt tcccaaatcg
    29821 cacgtaacgc atcgttgctc aggatggtgg caacaacaga agcgccgtgt gctggtgggt
    29881 tagagtagtt agcacgaatc gccgctttca tttggctgaa tgcgcgttca acagtttcac
    29941 tgtcagcagc aaccagagta caagcgccaa cacgctcgtt gtacaggcca aagtttttag
    30001 agtaggaact ggcaacaatc agctccttat gcagagccgc gaaagcgcgc agtccttcag
    30061 catcttcttc cagaccacgg gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc
    30121 ctttctcaac ggagagttgt gccagtgtct gccattgttc cagcgtaggg tcgatgccgg
    30181 ttgggttatg gcagcagcca tggaacagca ctacgtcgcc agcctgggct tcgttcaggc
    30241 tgttaatcag tgcatcgaag tcaagagtgt gattttccgc atcataataa gcgtattcac
    30301 gaacttccag acctgcagag ttaaagacgc tcttatggtt cggccagctt gggttgctca
    30361 cccacacacg cttaacgctg gtattttttg ccaggaaatc ggcagccacg cgtagtgcgc
    30421 cagtgccacc cggagtctgt gccgtgcgag cacgtttgtc attgatcagg gcgctacctt
    30481 taccaaacag cagttcctga gtgcagcgac caaattcagg gatgccgtca atgccgaggt
    30541 aatttttggt ggtttcattt tcgagcagat actgttcagc ctttttcacg ctggtcagta
    30601 ccggggtttt acccgtctca tctttataga caccaatccc gaggttaatt ttgccgggac
    30661 gttcatcggc acgaaacaga tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa
    30721 tgttctcaaa catgacgagg ttccattatg gttacagaag ggaagtccgc tatcagggta
    30781 acgggagatt tacaaaattc caactattac tgatgaaaac gcaggctgtt tttgcaagac
    30841 atgagattgc tctggaaggc ataaaaaaaa acaggaccaa agtcctgttt tttcggcatt
    30901 taacaaagag gtgtgctatt agaactggta aacgataccc acagcaacgg tgtcgtcaga
    30961 acctacgccc agtttgttgt cagaatcgat ctggttgatg atgtagtcaa cataggtgga
    31021 catgtttttg ttgaaatagt aggttgcgcc cacttcaaag tagttcacca gatcaacatc
    31081 accgatacct tctacgtctt tcgctttaga tttggtgtaa gcgatggacg gacgcagacc
    31141 gaaatcaaac tggtattgcg caactaacag aacgtcttgc gttttgttgg cgaagccgct
    31201 ggtgtttgta aatttattag tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc
    31261 caggtagatg ttgttcgcgt catacttcag accagtagcc cactgttcag cttttttgcc
    31321 tttgccaagg gaagactctt cctgcaggtt ggtacggtca gcggcaccat aagcaccaac
    31381 gataccaaag ccttcgtatt cgtagctgat agaaccgcca acaccgtcgc cgttagagcg
    31441 gcgtgcagtg tcacgctcgt ttttacccag gtactgaaca gcgaagttca gaccatcaac
    31501 cagaccaaag aagttggagt tacgataggt agcaacgccg ccaacacgac caacgaagaa
    31561 atcatcgctg tatgcagtat caccaccaaa ttctggcagc atatcggtgt aacccagtgc
    31621 atcataaacc acaccgtagt tacggccgta atcgaaagaa ccaacgtcag cgtatttaag
    31681 acccgcgaat gccagacgcg ttttgttacc agtttgagcg tcagcgccct cagagttgtt
    31741 accctggaag ttatattccc actgaccata accggtcaga tcggaattga tttgagtttc
    31801 ccctttaaaa ccaagacggg cataggtcat gtcgccattg ccaccgtaac tgttttcacc
    31861 gttacccttg gagaaataat gcagaccgac agctttaccg tacagatcta ctttgttgcc
    31921 atctttgtta tagatttctg cagcgtttgc agtacctgct actaacagag cagggacgat
    31981 cactgccaga atattgcgct tcatcattat ttattaccct catggttttt tttatgacac
    32041 ctgccactgc cgtcaataag ttctgtcaat aaaaatttac ggaactattg atgagagttt
    32101 ggtgtcttta tgtgtctgca ggcatctttc cattcaaact aacgtttcgc taccgtgaaa
    32161 gtgctacaaa gataaagatt tagtttcaaa aagaaaaaat atgtgacaaa aagtaaaaat
    32221 taaggaactt tgtgaacacc gtcatatttc catagaagcg tgatgatatt tacagcaatt
    32281 tttatctatt tatatgattt tcttatattt aaattaacta aacggaaatt ttgtttctga
    32341 tggaaacttt atcgaccagg cacaaaatct tctttcagac atccagaatg ccaaaaattt
    32401 gtatgaaaat tgtgctatca gctaagaaaa aactaatccg caataagaat gcagattatt
    32461 ttttgtgcgt aaatgttgca gcgtaatatt ccgtaacagg atgatcgtta tggcggggtt
    32521 gcaaatagat tgcttgactt aacaaacaga taacttgaca gaaaggataa aaaaagagcc
    32581 agcgggcgct ggctcttgaa gacgctaaat tagaagctgg cgttacgcgg agtacgtggg
    32641 aacggaataa catcacgtac gttttgcacg ccagtcacgt aagcaatcag gcgttcaaaa
    32701 ccaaggccga aacctgaatg cggaacagta ccgtagcgac gcagatcgcg ataccaccag
    32761 taatcttctt tgttcaggcc catttcaagc atacgctcgt ccagcacgtc cagacgttct
    32821 tcacgctggg agccaccaat gatctcaccg atgcccggag ccagaacgtc catagccgca
    32881 acggttttac cgtcttcgtt aaggcgcata tagaacgctt taatatcttt cggatagttt
    32941 ttaaccacta ccggtgcttt aaagtgttct tccgccagat aacgctcatg ctcagaagag
    33001 agatcgacgc cccagtaaac cgggttttca aacttcctgc cgcagttttc gagaatggtc
    33061 actgcgtcgg tgtaatccac ctgcgcgaaa tcggcctcaa tgaagcgttc cagacgtgaa
    33121 acggcatctt tatctacgcg ttcagcgaag aatttcatgt cgtcagcgcg ttcttcgaga
    33181 accgctttga agacatattt cagcatggct tcagccagac ccgcgatatc gttcaggtta
    33241 gcaaacgcca cttctggctc cagcatccag aattccgcca ggtgacggct ggtgttggag
    33301 ttttcagcac ggaaagtcgg gccgaaggta taaattttgg ataatgcgca agcgtaggtt
    33361 tcgccgttca actggccaga tacggtcagg aaagactctt taccaaagaa gtctttgtcg
    33421 aaatctactt tgccctgatc gttacgcggc aggttttcca gatccagcgt tgaaacacgg
    33481 aacatttcgc cagcaccttc ggtatcagat gcggtaatca gtggcgttga aacccagaag
    33541 aatccctgct cgttaaagaa gcgatgcagc gcctgcgcca gcgtatggcg aacgcgcgcg
    33601 acggcaccaa tcaggtttgt gcgcggacgc aggtgagcga cttcacgcag atactcaatg
    33661 ctgtggcgtt ttgccgccat cgggtaagtg tctggatctt caacccaacc agcaacttca
    33721 accttgctgg cctgaatttc aaattgttgc ccctggcccg gcgacgccac gactttaccc
    33781 gtcacaatga ccgagcagcc ggtggtcaga cgcaagacgt cttcattgta attgggcaga
    33841 gaattattga tgacagcctg tacaggatca aagcaggaac cgtcataaac ggcgaggaag
    33901 gagatgccag cttttgaatc tcggcgggta cgtacccatc cgcgcacggt gacttcgctg
    33961 tcaacggcta cgcggctctg gagtacgtcg gctacaggca caacgctcat aatattctct
    34021 ctgttaatag tcggaaaaaa taaacacttg tccacccgta aatgggggga tacctatgtt
    34081 acctggcatc tgcaatcaga caagcagaat tcgcaaatgc agcgaaagat ttcggaatta
    34141 aaggtaaaaa aagggagccg attagctccc tgatgatatt aactggcttt tttaatatgc
    34201 ggaaggtcga acgctttgcg cagcgcccga acaaacgctt tatcgtggca gatagttttg
    34261 ccagggctgt cagaaagttt cgccaccggt ttaccgttac actctaccaa cttaatgaca
    34321 atattcagag gttttacctg ggggatatcg caggtcaggc gagtcccaat accaaaactt
    34381 aattgcacgc gggaagagaa gtggcggtat agctcaaccg ctttgcgtaa atccagattg
    34441 tcagagaaaa ccagcgtttt actctgtgga tcaattccca gcttttcata atgtgcaatg
    34501 gctttttcac cccattcaac cgggtcacca gagtcatggc gcaggccctg atagcgactg
    34561 gcgaactcga caccgaaatc acgcaggaaa gcatccatag tgatgcagtc ggttaatgca
    34621 atgccaagtt ggtcgggata ctcttccagc caggcagcaa gtgcagctcg ctggctgttg
    34681 gctagatccg ggctgatttg ctgatgcgcc tggaaccatt cgtgtgcctg tgttcccatt
    34741 ggcgtgaggg aaagccgacg cgccagatcg tagttgctgg tgcccacgaa ccaggattcc
    34801 tgttgcagac gtttcacgat ggtttcttgt acttcgcgag aaaaacggcg gcgggtgcca
    34861 aaatccatca gatggaagcg cgacatatca agaccggcgg ttaatgccga gaagtcgact
    34921 aatttgtttt ccagcgtgtc gagggcttgc gcgacgtcgg cctgcggtga gcgatagcga
    34981 tgtaccattt cactgataac cgccagcaaa ggaacctccc agaggatgac ttcacgccac
    35041 gggccgctta aacgaatatc cagcttgcca ttatcgttgg acacggtgac ttgttccggg
    35101 ttaaagcgga actcgcgtag ccagttaaga tagtcggcct taaagaaagg cagggcagag
    35161 agccactgat attcatcatc ctgcaggcgc aggtgctgca tcgcctgaac ctgttcacga
    35221 atagcatcgg cataaatacc cagcagatcg tcacctcggc aacgaaactc cgccgcgaca
    35281 tgcacatcgt aatagtgatg aaacacggct tgctgcatat gcaacttata agcatctgta
    35341 tccagcaacg agtgcagaac aggagaagcg aattgtgtca taggtgcgca gtagcgtcct
    35401 cgtacaggag cgtttagtac aataaacatc ttcaggaact gctggagtat accttgtttc
    35461 gcaatttatt gaaccccgat cacaccatat gccacctttc tggtcgatgg cataacgcgg
    35521 tgtatgttat aaaaatgtag caataaaggc gtttgtacct gaaaagatga agattctgca
    35581 tagcgcgatt tacgcaacag gaatagactg aacaccagac tctataaaag atgctaaagg
    35641 ttatttatga ctcaacagcc acaagccaaa taccgtcacg attatcgtgc gccggattac
    35701 cagattactg atattgactt gacctttgac ctggacgcgc aaaagacggt cgttaccgcg
    35761 gtcagccagg ctgtccgtca tggtgcatca gatgttccgc ttcgtctcaa cggcgaagac
    35821 ctcaaactgg tttctgttta tattaatgat gagccgtgga ccgcctggaa agaagaagag
    35881 ggcgcactgg tcatcagtaa tttgccggag cgttttacgc ttaagatcgt taatgaaatt
    35941 agcccggcgg cgaatactgc gctggaaggg ctttatcagt caggcgatgc gctttgcacc
    36001 cagtgtgaag ccgaaggttt ccgccatatt acgtattatc tcgaccgccc ggatgtgctg
    36061 gcgcgtttta ccaccaaaat tattgccgat aaaaccaaat atcccttcct gctttccaat
    36121 ggtaaccgcg ttgcgcaagg tgaactggaa aatggacgtc attgggtaca gtggcaggac
    36181 ccgttcccga aaccgtgtta cctgtttgcg ttggtggctg gtgattttga tgtactgcgc
    36241 gataccttca ctacccgctc tggccgtgaa gtagcgctgg agctgtacgt cgatcgtggc
    36301 aaccttgatc gcgcgccgtg ggcgatgacc tcgctgaaaa actccatgaa atgggatgaa
    36361 gaacgcttcg gcctggagta tgacctcgac atctatatga tcgtcgcggt ggatttcttc
    36421 aatatgggcg caatggagaa taaggggctg aatatcttta actccaaata tgtgctggcc
    36481 cgcaccgaca ccgccaccga caaagattac ctcgatattg aacgtgttat cggccatgaa
    36541 tatttccata actggaccgg caaccgtgtc acctgtcgcg actggttcca gctcagtctg
    36601 aaagaaggtt taaccgtctt ccgcgatcag gaattcagct ctgaccttgg ttcccgcgcg
    36661 gtaaaccgta tcaataatgt gcgcaccatg cgcggattgc agtttgcaga agacgccagc
    36721 ccgatggcgc acccaattcg cccggatatg gtcattgaaa tgaacaactt ctacaccctg
    36781 accgtttacg agaagggcgc ggaagtgatt cgtatgatcc acactctgct tggcgaagaa
    36841 aacttccaga aagggatgca gctctatttc gagcgtcatg atggtagcgc agcgacctgt
    36901 gacgactttg tgcaggcgat ggaagatgcg tcgaatgtcg atctttccca tttccgccgt
    36961 tggtacagcc agtccggcac gccggttgtg actgtcaaag acgactacaa tccggaaact
    37021 gagcagtaca ccctgaccat cagccagcgc acgccagcta cgccggatca ggcagaaaaa
    37081 caaccgctgc atattccgtt tgccgtcgaa ctgtatgaca acgaaggcaa agtgatcccg
    37141 ttgcagaaag gcggtcatcc ggtgaattcc gtgctgaacg tcacccaggc ggaacaaacc
    37201 tttgtttttg ataatgtcta cttccagccg gtgcctgcgc tgctgtgcga attctctgcg
    37261 ccagtgaaac tggaatataa gtggagcgat cagcaactga ccttcctgat gcgtcatgcg
    37321 cgtaatgatt tctctcgctg ggacgcggcg caaagtttgc tggcaaccta catcaagctg
    37381 aacgtcgccc gtcatcagca agggcagccg ttgtctctgc cggtgcatgt ggctgacgct
    37441 ttccgcgcgg tgctgctcga tgagaagatt gatccggcgc tggcggcaga aatcctgacg
    37501 ctgccttccg tcaatgaaat ggcagaactg ttcgatatca tcgacccgat tgctattgcc
    37561 gaagtacgcg aagcactcac tcgtactctg gcgaccgaac tggcagatga gctgctggct
    37621 atttacaacg cgaattacca gagcgagtac cgtgttgagc atgaagatat tgcaaaacgc
    37681 actctgcgta atgcctgcct gcgcttcctc gcttttggtg aaacgcatct ggctgacgtg
    37741 ctggtgagca agcagttcca cgaagcgaac aatatgaccg atgcgctggc ggcgctttct
    37801 gcggcggttg ctgcacaact gccatgccgt gacgcgctga tgcaggagta cgacgacaag
    37861 tggcatcagg atggtctggt gatggataaa tggtttatcc tgcaagccac cagcccggcg
    37921 gcgaatgtgc tggagacggt acgtggtctg ttgcagcatc gctcatttac catgagcaac
    37981 ccgaaccgca ttcgttcatt gattggcgca tttgcgggca gcaacccggc agcgttccat
    38041 gccgaagatg gcagcggtta tcagttcctg gtggaaatgc ttaccgacct caacagccgt
    38101 aacccgcagg tggcttcacg cctgattgag ccgctgattc gcctgaaacg ttacgatgcc
    38161 aaacgtcagg agaaaatgcg cgcggcgctg gaacagttga aagggctgga aaatctctct
    38221 ggcgatctgt acgagaagat caccaaagca ctggcttgat acttttgagc caaaatgctt
    38281 gacgcgtctt atcaggccta caagtccgtt gaaattgctg gttttgtagg ctggataagg
    38341 cgttcacgcc gcatcaggca gtcgtgccca actgcctgat gcgacgcttg acgcgtctta
    38401 tcaggcctac aatcccgttg aaaatgctgg ttttgtgcgc cacatccggc atattaacca
    38461 tgacgtcgaa tcggttgttc agagtgccca cgttgcatca cccgctgcaa cacttccgct
    38521 tccagctctg ctaatcgtac cgaccctaac cggcgcggac gggggatatc caccgtcaaa
    38581 tccagaccaa tttttccctc ttcaattaac aacacccggt cagccatcgc caccgcttcg
    38641 ctcacatcat gcgtcaccag caaaacggta aaaccgtgct tcagccaaag cgacacaatc
    38701 aaatcctgca tctcgagtcg cgttaaagcg tccagcgcac cgagtggttc atcaagcaac
    38761 aataaacctg gtcgatgaat cagcgccctt gccagcgcca cacgttgttt ctgcccgcca
    38821 gaaagtgcgg caggccattc ccccgcgcga ttctccagtc ctaccgcagc cagagcctga
    38881 cgcgccgcat cgcgccactg gcctttaagg cctaacgcaa cgttatcaat caccgatttc
    38941 cacggcagca gacgcgcgtc ctgaaacatc attcgcgtat catcctgaat ctcagccagc
    39001 ggtgtggtgc ccgctaacac gtcgcctgcg gttggcgttt ccagacctgc cagcaggcgc
    39061 agcagagtac ttttgccacc gccgctgcgg cccaccaccg ccacaaactg acctgccgga
    39121 atatgtaaat ccagttggtt caggacgata ttttccgcgt aatgtttgct tactgcattg
    39181 agcaacaatg gtgtgccctg gttcagacga gccgtattca taccgtggcc tccttcaaat
    39241 gataagccgg gttccagcgc aaccagagac gttctaacag ctgcgcgctg acgtcagcaa
    39301 gtttgccgag cagggcgtaa agaataatag cgactaccac cacgtccgtt tgcagaaact
    39361 ccagtgcatt catcgccaga taaccaatgc cggaattggc agaaatagtt tcggcaacga
    39421 tcagcgtcag ccacatcagg cccaacgcaa aaagcacacc gaccataatt gagggcaggg
    39481 caccaggcag gatcacatgg ataaacagcg gtatgccgga taatccatag ctacgtgcca
    39541 tctccaccag cccacggtcg atattacgga tgccatgcca ggtgttgata taaatgggga
    39601 atagcgtacc gagcgccacc agaaatattt ttgcggattc atcaatgccg aaccacaaaa
    39661 tcaccagcgg gatcagcgcc agatgcggca cgttgcgcaa catctgaatg gaggtatcca
    39721 gcagccgttc tccccagcgc gacagcccgc taatcagccc cagaattaat cccagcgatc
    39781 cgccaattga aaaaccaatc agcgcacgcc aggagctgat cgccagatgc tgccacagtt
    39841 cgccgctggc agagagcgtc cagaacgcca tcaccactcc ttccggtgag ggcaaaatac
    39901 gcgtcgatag ccagccaacc gaggaggcca gttgccacac cgccacgatg cccaccggta
    39961 aaaaccaggg agcaacgcgc aataaccact tcttcactgg cgttgccatc atcgtgttcc
    40021 ttagctttgc gcgacgttac gggggataaa atcattcgcc accgcttcgc cttgtggatt
    40081 cagcggttgt ggctggggaa tttccgggat ggcgacatcc agatgtggga acagtaactc
    40141 gccgacccga tacgcttctt ccagatgcgg gtagcccgaa agcacaaaac tgtcgatgcc
    40201 aagtgcagcg tattcgttga ttcgcgcgac gaccgtagga ccatcgccca ccagcgccgt
    40261 cccggcaccg ccgcgcacta agccaacacc cgcccataaa ttggggctga tctccagatt
    40321 gtcgcgcttg ccgttatgta gcgccgccat tcgctgttgt cctacggaat ccgtgcgggc
    40381 gaatgcggcc tgtgctttgg cgatagtttc atcatcaaga tgcgagatta accgctcggc
    40441 ggcctgccac gcttcgtcgt tggtttcacg aacaatcaca tgcagacgaa taccgaaacg
    40501 aattttgcgt ccatgcgcgg cagctttcgc ccgcacgtgt tcgattttct cttttaccag
    40561 ttccggtggt tcgccccagg taaggtagag atcaacttgt tctgccgcca gctcctaggc
    40621 gacatctgac gatccgccaa agtaaagcgg aggataaggc tgttgaatcg gcgggaagag
    40681 cagtttggct ccgcgtacat gaatatgttt gccgttgaaa tccacagtct cgccaagcaa
    40741 caaccgccgc cagacctggg taaattctgc cgaggcttcg taacgctcgc tatgatcaag
    40801 gaacacgccg tcgcctgcca gttcttgtgg atcgctgcct gtgaccaggt taaacaacgc
    40861 acgtccgtta gagagacggt caagcgtggc ggcctgacgg gcggcaacgg taggtgaggt
    40921 tacgctgggg cgcagggcga caagaaactt cagccgttgt gtcaccggga tcatcgacgc
    40981 cgccaccagc cacgcatctt cacacgatcg ccccgtcggg atcagcacac cggtatagcc
    41041 cagacgatcc gccgcttgcg caatttgttg cagataaccg tgatcaaccg gacgtgaacc
    41101 ttcttccgtt cccagataat gcccgtcacc gtgggtcggt aaaaaccaga acatattcag
    41161 actcataatt gttttccttc cagttgagtg ggctgccaga tgcgctggcg aatatcgact
    41221 tttttcggca ccagacggtt ttcataaaac agatctgccg tttgctgctg taaggcggca
    41281 acttcagcgc tcaacggttt gatggtggtg ggaggacgat gatccagata tgaggcaata
    41341 accggtgccg gtaagcccat cgtttttgcc agtaaagcga tgctttgttc gcgctggctg
    41401 cgggttaacg catcggcctc actaaaggtt gtcagcacac cctgaataaa agcgccgttt
    41461 ttttctgcat acggacgagc tgccagataa aacgatccag tttgattgag atcggtgccg
    41521 tctttcagca cccgcacgcc gccctgtaat aatgcagcgg agtagtaggg atcccagata
    41581 gcctaggcgt caacgttacc ttgctggaac gcggcgcggg catcggcagg cgtcaggtaa
    41641 gtgggctgaa tatccgtgaa tttaagcccg gcctggcgaa gggctcgcag taaaagattg
    41701 tgcgaactgg aacctttctg aaaggcaact ttgtgacctt taagatcggc tacggttttg
    41761 atcgggctgt tttctgccac cagaatcact tcggctttgg gcttcggtgg ctcgacgccc
    41821 acgtatacca aatcagcccc ggcagcctgg gcaaagattg gcggaatatc cccggtactg
    41881 ccgagatcga tactgccaac gtttaacgcc tccagcatct gtgggccagc ggggaactcc
    41941 acccaggaga tttttgtttg cggatagcgt ttttccagta actggtggct ttttgccagc
    42001 accataccaa tactgccttt ttgatagcct atacgtaacg cctctggcga agactccgcg
    42061 gcaacagcaa gtgtagagac gctaagtaaa cccgccagcg ccagtttaat gatcttacgc
    42121 atgggcatta cctcgcagag acagaaggtc aggaacctga acatcgcggc ggtgcaacgc
    42181 ctgccagaaa gtttctagcg cggtatcaag acgagtttgc agatttggcg tgaactgggg
    42241 tttgtgttgg taatcaatga cttgcgagtc atcggcaaac acgccgtgca ggatctcctg
    42301 ggctttcagc gtacttaaaa ctggcttaag ggcataatcg accgccagca gatgggccac
    42361 ggtgccgcct gtcgccagcg gcagcaccac tttgccttgc aacgcacgtt ctggcagcag
    42421 gtcgagcagg gttttcagtg caccggaata ggcggcttta tacacaggcg tggcgacaat
    42481 cagcccatcg gcctgttgca gctgttcggt gaaggtcttg agtgccggac tatcgaaacg
    42541 agcataaagc agatcttccg ggacgaagtt ttgcaaattc cagtgataaa cctctacatc
    42601 caggccattt agtttttccc gcgcatattc cagcaaggag ctggagcgag aaggaaagcg
    42661 aggactaccc gccagggtga tgacacgcat actctctcct tataaccaat tgttcttgtt
    42721 ttgttaacat tgataacaat tcggtcagtc tgacagaggg cgtccgcgag tcgaaatgat
    42781 ttatctgagg aagaaaagcc aaaaaaagca taaaaaaagc gcaataagaa aacgtttgct
    42841 atttattgcc gcaggtcaat tcccttttgg tccgaactcg cacataatac gcccccggtt
    42901 tgcacaccgg gaatccagga gagttcatgt actacccctt cgttcgtaaa gcccttttcc
    42961 agctcgatcc agagcgcgct catgagttta cttttcagca attacgccgt attaccggaa
    43021 cgccgtttga agcactggtg cggcagaaag tgcctgcgaa acctgttaac tgcatgggcc
    43081 tgacgtttaa aaatccgctt ggtctggcag ccggtcttga taaagacggg gagtgcattg
    43141 acgcgttagg cgcgatggga tttggatcga tcgagatcgg taccgtcacg ccacgtcccc
    43201 agccaggtaa tgacaagccg cgtctctttc gtctggtaga tgccgaaggt ttgatcaacc
    43261 gtatgggctt taataatctt ggcgttgata acctcgtaga gaacgtaaaa aaagcccatt
    43321 atgacggcgt tctgggtatt aacatcggca aaaataaaga tacgccggtg gagcagggta
    43381 aagatgacta tctgatttgt atggaaaaaa cctatgccta tgcgggatat atcgccatca
    43441 atatttcatc gccaaatacc ccaggattac gcacactgca atatggtgaa gcgctggatg
    43501 atctcttaac tgcgattaaa aataaacaaa atgatttgca agtgatgcac cataaatatg
    43561 tgccgatcgc agtgaagatc gcgccggatc tttctgaaga agaattgatc caggttgccg
    43621 atagtttagt tcgccataat attgatggcg ttattgcaac caataccaca ctcgatcgtt
    43681 ctctggttca gggaatgaaa aattgtgatc aaaccggtgg cttaagtggt cgtccgcttc
    43741 agttaaaaag caccgaaatt attcgccgct tgtcacagga attaaacgga cgcttgccga
    43801 ttattggtgt tggcggcatt gactcggtta tcgctgcgcg tgaaaagatt gctgcgggtg
    43861 cctcactggt gcaaatttat tctggtttta tttttaaagg cccgccgctg attaaagaaa
    43921 tcgttaccca tatctaatta tttcttcaaa ttcctcttta taagataatc agggctttat
    43981 tttcagccct ggttgtttta tattcatcgc tgttgcttat ttagacattt tgtactttta
    44041 ttgttgaggt tattaagcga agcgacaatg gattgtgtcg ctgcggtttt ataaggttgg
    44101 ggagtggcag atgcgaatta aaccagacga taactggcgt tggtattacg atgaagagca
    44161 cgatcgaatg atgctcgatt tagccaatgg tatgctattt cgctcacgtt ttgcgcgcaa
    44221 aatgttgact ccagatgcct tttctcccgc aggtttttgc gttgatgacg ccgcgctcta
    44281 tttctctttt gaagaaaagt gccgcgattt taatttatct aaagaacaaa aagccgaact
    44341 ggtgctgaat gcactggtag caattcgtta tctcaaaccg caaatgccga aaagctggca
    44401 ttttgtatct catggtgaaa tgtggatgcc catgccgggc gatgcggcct acgtttggtt
    44461 gagcgacact catgagcaag tgaatttact ggtcgttgaa tctggcgaga atgccgcgct
    44521 atgtctactg gcacaaccct gcgttgttat tgcgggtcgc gcgatgcagt tgggcgacgc
    44581 cattaaaatc atgaacgaca ggctgaaacc gcaggttaat gttgacagct tcagcctcga
    44641 acaggcagtc taacgcgcca gcttaagcgc agtcttcggt acacaactac agcaaagaat
    44701 agtgccatca tcgcctattg ctgatttttt tagcggcgtg acttcgcctt ctaaaagctg
    44761 aacacggcaa attccacaaa tgcccgcgcg gcaagaataa gggatacgaa ttccctgatt
    44821 ttctaattgc tccagcagca cctgttggtt atttccacga aatgcctgtc cctgccagtc
    44881 aatatctacg tttgcgtccg gttgttgcgt gatgttggca gtatcatcag cggcacctgc
    44941 gccgtaaatt ttagccggag ccgttgccag aatttccacc tcgtcgccaa cgcgaatcac
    45001 gccgctatta cgggcaatta aattctgacc aaaatcgaca tcgccgttat cctgggcagt
    45061 acggaaagat tgcagcgttt ttaatggttc gcctgccgga tgtttttgcc ctttttctgg
    45121 gctgacggtg gtgaaaatac agcggctaca aggcttaacc acatcaaaca cgacatcacc
    45181 aatgcgaatc actttccagc tatcttcttc ccaggccgaa gcaccggaaa ccaccagatt
    45241 ggggcggaat tgctccattt ttacactggc cggacaacgt tgttggagat cacgtaacga
    45301 ggcctcgtta gcaagaaggt aaggatagcc atcagcaaat gacaggggta cagtgttgtg
    45361 gcgtttcacg cgccgggtca tttgtggccc cacccagcgt aattgcactt cgcgggagaa
    45421 aaatccactt agccatttgt tgatcgcgtc tggcgcaatt cgcgcggtaa aatgtgtgcc
    45481 ccaaacttcg gttggtgcgt cttgtgtggc gaaatcagca aaacgaacat atgcactact
    45541 gccatctggt gcggtgagat gcaagccatc atgcacgggt gaaggagtaa accgtaccat
    45601 tagcggaaac tggcgagcgg taataaacgt accgtcaggt tccgtgatca taaagatgcg
    45661 atcgaaggcc agaccactga catctgccag ggtatgtgta agaccaatgc cgcgcatcga
    45721 tttaacagga tgaataaaaa gccgggttaa tgtcgccacg gtgcggtcct caaatgaaaa
    45781 taagccctca actttatgac atgacgcgct tattagctat aatgcgcaac aattttctta
    45841 gagtaaaagt gacaatatga attctctgtt tgccagtacg gcccgtgggc tggaagagct
    45901 gttaaaaact gaactggaaa acctgggggc cgttgaatgc caggtggttc agggtggggt
    45961 ccatttcaag ggcgacacac ggcttgttta ccagagcctg atgtggagcc gcctggcctc
    46021 gcgtattatg ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc tctatctcgg
    46081 tgttcaggcg atcaactgga cagagatgtt taatcctggc gcgaccttcg ctgtccactt
    46141 cagtggtttg aatgacacca tacgcaacag tcagtacggt gcgatgaaag tgaaagacgc
    46201 gatcgtcgat gctttcacgc ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc
    46261 ggatatccgc gttaacgtct ggctgcataa agaaaccgcc agtatcgccc ttgatctcag
    46321 tggtgatggt ttacatctgc gtggctatcg cgatcgtgct ggcattgcgc cgatcaaaga
    46381 aaccctggca gccgctattg tgatgcgatc cggctggcag ccaggaacac cgctgctcga
    46441 tccaatgtgt ggttcaggta cgttgctgat tgaagcggct atgttggcga ccgaccgcgc
    46501 accgggcttg caccgtgggc gttggggctt tagcggctgg acgcagcatg atgaagctat
    46561 ctggcaggaa gtgaaagcgg aagcgcaaac tcgtgcccgt aaaggcctgg cggagtatag
    46621 ctcccatttc tacggttcgg acagtgacgc acgggtgatt caacgtgcgc gcactaacgc
    46681 ccgtcttgcg gggattggtg aactgatcac ctttgaggtg aacgatgtcg cgcaactggc
    46741 caatccgctg ccgaaagggc cgtacggtac agtgttgagc aacccgccat acggtgaacg
    46801 tctggacagc gaaccggcgc tgattgcgct gcatagtctg ctgggccgaa tcatgaaaaa
    46861 ccagttcggt ggctggaatc tctctttgtt tagtgcctcg ccggatctgc taagctgcct
    46921 gcagctgcgt gcagacaaac agtacaaggc gaaaaacggc ccgctggact gcgtacagaa
    46981 aaactaccat gttgccgaaa gcacaccaga cagcaaaccg gcaatggcag cggaggacta
    47041 cgccaaccgt ctgcgtaaga acctcaaaaa attcgagaag tgggctcgcc aggaaggaat
    47101 tgaatgttac cgcctgtatg acgccgatct gccggaatat aacgttgctg ttgaccgtta
    47161 tgccgactgg gtggtggtgc aggagtatgc gccgccaaaa actattgatg cgcacaaagc
    47221 gcgtcagcgt ctgttcgata ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa
    47281 caaactggtg ctgaaaaccc gtgaacgcca gaagggcaaa aaccagtacc agaaactggg
    47341 cgagaagggt gagtttctgg aagttaccga atataacgct cacttgtggg tgaacctgac
    47401 ggattacctc gataccggtc tgttcctcga tcaccgtatc gcccgtcgta tgcttggtca
    47461 gatgagcaaa ggcaaagatt tcctcaacct gttctcttat accggcagcg ccaccgtgca
    47521 tgcgggatta ggcggtgcac gcagcaccac caccgtggat atgtcgcgta cttatctgga
    47581 gtgggcagaa cgcaacctgc gtctgaatgg cttaactggc cgtgcgcatc gcctgattca
    47641 ggccgattgc ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga tctttatcga
    47701 tccgccaacc ttttctaact caaaacgaat ggaagatgcg tttgatgttc agcgcgatca
    47761 tctggcgctg atgaaagatt tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc
    47821 gaacaacaaa cgcgatttcc gtatggatct cgacggtctg gcgaaactgg gactgaaagc
    47881 acaagaaatt acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc agatccacaa
    47941 ctgctggctg attaccgcag cctgaaagga atagtaatgt cattaatcag tatgcatggc
    48001 gcatggctgt cgttcagcga cgcgccgctt ctcgataacg cagaactgca tatcgaagat
    48061 aacgaacgtg tttgtctggt ggggcgtaac ggcgcaggca aatcgacgtt aatgaagatc
    48121 ctcaaccgtg aacaggggct ggatgacggt cgcattattt atgagcaaga tttgattgta
    48181 gctcgtctgc aacaggatcc gccgcgtaac gttgagggta gcgtttatga tttcgttgcc
    48241 gaaggcattg aagaacaagc ggaatacctg aaacgctatc acgatatttc gcgcctggtg
    48301 atgaacgacc cgagcgagaa aaatctcaac gaactggcga aggttcagga acagctggat
    48361 caccacaacc tgtggcagct ggaaaaccgc atcaacgaag tgctggcgca actaggatta
    48421 gatcctaatg ttgcgctgtc gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga
    48481 cgcgcgctgg tgagcaatcc gcgcgtattg ctgctcgacg aaccgactaa ccacctggat
    48541 attgaaacca tcgactggct ggaagggttc ctcaaaacct tcaatgggac aattattttc
    48601 atttcccacg accgttcgtt tatccgcaat atggcgacgc gcattgttga tctcgatcgc
    48661 ggcaagctgg tgacctatcc agggaattac gaccagtacc tgctggaaaa agaagaagcc
    48721 ctgcgcgtgg aagaattaca caatgccgag ttcgatcgca aactggcgca ggaagaggtg
    48781 tggatccgcc aggggatcaa agcacgtcgt acccgtaatg aaggccgcgt acgcgccctg
    48841 aaagcaatgc gtcgtgagcg tggtgaacgt cgcgaagtaa tgggtaccgc aaagatgcag
    48901 gtggaagaag ccagccgctc cggtaagatc gttttcgaaa tggaggacgt ttgctaccag
    48961 gttgacggta agcaactggt gaaagatttt tctgcccagg ttctacgcgg cgacaaaatt
    49021 gccctgattg gtccgaatgg gtgcggcaaa accacgctgc tgaaactaat gctcggtcag
    49081 cttcaagcag acagcgggcg tattcacgtt ggcaccaaac tggaagtggc ttatttcgat
    49141 cagcaccgcg cggaactgga tcccgataaa acggtgatgg ataaccttgc cgaaggtaag
    49201 caagaggtga tggttaacgg caagccacgc cacgtattgg gctatttgca ggacttcctg
    49261 ttccatccaa aacgggcgat gacgccggta cgtgcgcttt ctggcggtga gcgtaaccgc
    49321 ttgttgttgg cgcgtttgtt cctcaaacca agcaacttat tgattcttga cgaaccgacc
    49381 aacgatcttg atgtcgaaac gctggaactg ctggaagaac tgatcgacag ctatcagggc
    49441 acggtattgc tggttagcca cgatcgtcag tttgtcgata acaccgttac agaatgctgg
    49501 atcttcgaag gcggcggtaa aattggtcgt tatgtcggcg gttatcatga tgcccgtggt
    49561 cagcaagaac agtatgtggc gctcaaacag cctgcggtga aaaaaagcga agaagccgct
    49621 gcgccaaaag cagaaactgt aaaacgcagc agtagcaaac taagctataa attgcagcgc
    49681 gaactggagc agctaccgca attgcttgaa gatctggagg cgaagctgga agctttacag
    49741 acgcaagtgg cggatgcttc cttcttcagt cagccccatg agcagacaca aaaagtgctc
    49801 gccgatatgg ctgctgcaga gcaggagctg gagcaagcct ttgaacgctg ggagtatctt
    49861 gaagcgttaa aaaatggtgg ctgatcgcaa aatagtcgat ttaaacagag acagctgaca
    49921 acggtaaaat tgcctgatga actgggttta tcaggcatga aaaccaacac tttgttagtc
    49981 atctgcaaaa tagcgcatca ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag
    50041 cacatcctgt gctcgcagtg tgacatgctg gtggcgttac cgcgccttga gcatggtcag
    50101 aaagcggcat gtccccggtg tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag
    50161 cgtcccaccg cctatgcgtt ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct
    50221 tttgtgaata tgaacgttgc aggagttacc agtgaaatta cattactgga aattcccggc
    50281 gtgctttttt ctgaggacta cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg
    50341 gttcccgcgt tttgtctgat aaccattctg ttactggtga atcgcgcgga attaccggtc
    50401 cgtttaaaag agcaactggc acgggtgctt tttcaactca aaacctgggg aatggcggag
    50461 attttcctcg cgggtgtgct ggtcagtttc gttaaactga tggcttacgg cagcattggg
    50521 gtaggcagca gctttctccc ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag
    50581 tgcgttgatc gtcgctggtt atgggacgac atcgccccga tgccagaact gcgccagccg
    50641 ctaaaaccag gtgtcacggg gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca
    50701 atccttcctg ctgatgaacc cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt
    50761 agaaacagcc tgcagtggac actcgcgctg cttgtaacgt ccatcatgct gtatcttccg
    50821 gctaatattt tgcccatcat ggtgacggat ttattaggct cgaagatgcc gtcgacgatt
    50881 ctcgctgggg tcattctgtt atggagcgaa ggctcttatc ccgtcgctgc ggtgattttt
    50941 ctggccagta ttatggtgcc aacgttaaag atgatcgcca ttgcgtggct gtgttgggat
    51001 gccaaaggac acggcaagcg cgacagtgaa agaatgcatt tgatttatga agttgttgag
    51061 tttgtaggcc gctggtcgat gattgacgtt ttcgttatcg cggtgctctc ggcgctggta
    51121 cgtatgggag gtttaatgag tatttatccg gcaatgggtg cattaatgtt tgctttagtc
    51181 gtcataatga caatgttttc tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa
    51241 cctgaatcag agcatgagga gtcctgacag tatggaatct aataatgggg aagccaaaat
    51301 ccagaaagtg aagaactggt ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg
    51361 ggcctgggtt cttttttatc attacagcca tcagggaccg gaagtgaccc tgatcaccgc
    51421 gaatgcggaa ggaattgaag gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg
    51481 cgtggttgaa agcgccacac tggctgatga tttgacacac gttgaaatca aagcgcggct
    51541 gaattccggt atggaaaaat tgctgcataa agataccgtc ttttgggtgg tgaaaccgca
    51601 gattggtcga gaagggatta gcggcctggg aacgctgctg tctggggttt atatcgaact
    51661 gcagccaggc gcgaaaggca gcaaaatgga taaatacgat ttgctggact cgccaccgtt
    51721 ggccccgcct gatgcgaaag gtatccgtgt gcttctcgat agcaaaaaag ccgggcagct
    51781 ctcgccagga gatccggtgc tgttccgtgg ctatcgggta ggttcggttg aaaccagcac
    51841 tttcgatacg caaaaacgca atatcagcta tcaactgttc atcaatgcac cttatgaccg
    51901 actggtgacc agcaatgttc gtttctggaa agatagtggc attgcggttg atctgacgtc
    51961 agcgggaatg cgtgtggaga tgggctcatt gacaacgctg cttagtggcg gtgtcagctt
    52021 tgatgtgccg gaaggtctgg atttagggca gccagtggca ccgaaaacag ctttcgtttt
    52081 gtatgatgat cagaagagca ttcaggattc gttgtacacc gatcacattg attatctgat
    52141 gttctttaaa gattcggtac gcggtctgca accgggagct ccggtagaat tccggggtat
    52201 tcgcctgggt accgtaagca aagtgccatt ctttgcgccg aatatgcgtc agacatttaa
    52261 cgatgattac cgtattccgg tactgattcg tatcgagcca gagcggctga aaatgcagct
    52321 tggcgaaaat gcggatgttg ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg
    52381 atcgctgaaa accggaaacc tggtcactgg cgcactgtat gttgatctcg atttctatcc
    52441 aaatacgcct gcaataaccg gtattcgtga atttaatggt tatcagatta tcccgaccgt
    52501 cagtggcggc ctggcgcaaa tccagcaacg actgatggaa gcgttggata agatcaacaa
    52561 actgccactg aatccgatga ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac
    52621 aatgaaaaac ctgcaaacga cgctggatag catgaacaag atccttgcca gccagtcgat
    52681 gcagcagtta ccgacggata tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg
    52741 cttccagcct ggttccgcag cctacaacaa gatggtggcg gatatgcagc gccttgatca
    52801 ggtgttgcga gaactgcaac cggtgctgaa aacgctcaac gagaagagta acgcgctggt
    52861 atttgaagcg aagggcaaaa aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg
    52921 ctagtgacga ttgcggcact gtggctggcc ggatgcagct ccggcgaaat taataaaaac
    52981 tattaccagt tacctgtggt gcagagcggt acacaaagca ccgccagcca gggtaatcgc
    53041 ctgttatggg ttgagcaggt cgctgttcct gactatctgg cggggaatgg tgtggtttat
    53101 caaaccagtg atgtgaagta tgtgattgcc aacaacaacc tgtgggccag cccgttggat
    53161 caacagttgc gcaacaccct ggttgccaac ctgagtacgc aactacccgg ctgggtggtt
    53221 gcctcccagc ctctgggaag cgcccaggac acgctcaatg ttaccgtcac ggagtttaac
    53281 ggtcgctatg atggcaaggt cattgtcagt ggtgagtggc tgttgaacca ccagggacaa
    53341 ctgatcaaac gtccgttccg tctggaagga atgcaaactc aggatggtta cgatgagatg
    53401 gttaaagtgc tggccagtgt ctggagtcag gaagccgctt ctattgcgca agagataaag
    53461 cgtctacctt aatcataaag atttgtaaat ataaccgcct ccggtatgtt gcctgaggcg
    53521 tttttttgtt tctaacgtgc ggaaaaagtt gttcctcttc acattttttg tacaaccgac
    53581 atgcccgtgt agctcacaaa tatgacagtg gcgtgaattt tgcgcattga cggcagttat
    53641 gattcgcggt attgcttaac tgtgattgca tatttagtaa tcactgtttt cttttccacc
    53701 agaaaccagt atgagggaaa cgaggcatga agagacaaaa acgagatcgc ctggaacggg
    53761 cacatcaacg tggttatcag gccggcatcg ccggacgctc aaaagaaatg tgtccctatc
    53821 agacgctgaa tcaaaggtca caatggctgg gaggctggcg agaagccatg gcggacaggg
    53881 tagtaatggc ctgattctgt ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct
    53941 ttatatactc tgtctgatta aaatcagaat gcagacgtat cctggaacag accgactttc
    54001 aggtcgttgg cggtatagat caaacgacca tcaaccagca cttcgccatc agccaggccc
    54061 ataatcagac gacggttaac tatgcgttta aagtgaatac gataggtcac ttttttcgcc
    54121 gtcggcagca cctggcctgt gaatttcact tcacctacgc ccagcgcgcg acctttacct
    54181 tcgccgccca gccagccgag gtagaaccct accagctgcc acattgcgtc caggcccagg
    54241 catcccggca taaccggatc gccgataaag tggcatccga agaaccacag atccggattg
    54301 atatccagct ctgcttcaac ataccctttg tcgaagttac cacccgtttc ggtcattttg
    54361 accacacggt ccatcatcag catgtttggt gctggcaatt gcgggccttt agcgccaaac
    54421 agttcaccgc gaccagaggc aagaaggtct tcttttgtat aggattcgcg tttatctacc
    54481 atgttctctg taagccttat tttattgaag cacgcaggat agctaacacg tgtacgctga
    54541 acaagtccga tcagttcgga ataaaccagt tcagccaacg taatggccat ggaaaacggt
    54601 gacgtccttc ctgttgcgat gcttgtgcga tacgttcctg gatggtttgc atcagcgtcg
    54661 tttggccttc gccatcccac accagattta ataataacgg cagtgcgtca gtcacatcgt
    54721 ctactgccca gatggtgaat ttgtcttctt ctaccgcttt caccagttca ctgtgaagac
    54781 ttaaatggcg aacgttagct gtggggataa tgacaccttg tttccccgtt aactcacgtt
    54841 gctggcaaat agcaaagaag ccttcgattt tctcatttaa accaccgact ggctgggcgc
    54901 gaccgaactg atcgactgaa cctgtgatag cgatactctg attcaccggc acatcggcga
    54961 gggcgcttat cagggcgcag agttcagcca tcgaggcact atcgccatcc acttcactgt
    55021 atgactgctc aaatgtcagc gatgctgaga aggggatctg ttgctcaagc tgtagttccg
    55081 acatcaggaa cgcttgcatg atcatcatcc ctttcgcatg gatattgccg ccaagctccg
    55141 ctttgcgttc gatgtcggtg aattcaccat cgccaatatg cacaacgcag ctaatgcgag
    55201 aaggttcgcc aaaagcgcgt ggatgacccg gaaattcaat gaccgaaagg gcgttaattt
    55261 gcccgatgcg ttcgccttcg gtttcaatca ggatttgctc ctgaaggatc tcatcctgca
    55321 tgcgttcagc gaggaaacct tcgcgccatt cacgctgctg tagcattaag tttagctgct
    55381 cgccggagaa ggtgtcgcca tcacacaggg aggcgacctc ttgacactgg cggaggatcc
    55441 actgcgggct aagcggaagt gtttcttgtt caccagtgta gcgtgctgct tcgcggataa
    55501 gtaccggcca ggcatccgct cccggtgctg gtaagtgatt atgtttggcg gtaaatgtca
    55561 cccagcgaca ccactggctt actgactccg catcgacaat ctgcagagta tcttcaaatt
    55621 cgctataaat agtctgctct gaaagctctg gctccatctc ctggaaatca gccaatgatt
    55681 cgcgttcgcc taccagaatg actttcagct tcaatggcat cgaaggcaca gagacgggga
    55741 gagggcgcga ctcatcaaac gcaacccagt caaaacgctc gcggttaacg atatttttca
    55801 gccgcatcca cagcagaggt tgcgccagca gtgtacgcaa agagatgatg agaataccgc
    55861 catttgcctg atgcaccaga ccaggctgca gggtaatgtc gccattaaac tgacgcaggc
    55921 agccaaagag ttgctctgct tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa
    55981 aattatcatc tgcactcact gcgtgacgta agcggatggt gtggccggaa acctcatagt
    56041 gaccgcccac cagttggcct gcatcgcttt gtagcgtacg cgcggcatca gcaataagat
    56101 tcagatactc agactcttcc ggggccttcg ccagcataaa agaggaggat gctcgcgtat
    56161 gcagcaactg ctccagcgca aattgcagtc gcggttgagt atcactgaat aaaggatcgt
    56221 tttcgtcaat caaatgtggc tgagcaaata tttcctgata gctatcggta tcaggaacca
    56281 ggtcacgcca tgcaagtttc gtaatggtca aagttgatgt tttttagtct gttgtcaaag
    56341 ccgcgattat accgtaaccg gcactgcagc acacgtagaa agcaacgaca atactcttgg
    56401 catgggcgtt aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata
    56461 ttctgtaaag agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat
    56521 cttgaaagcg gttggaaatg gaagtatctg gtgaagaagc accgcgaagg ggagttaatc
    56581 acccgttaca tagaagccag tgccgcccag gaagccgtcg atgagttgct ctcgctggaa
    56641 aatgaacccg tgctggtaaa tggttggatt gacaagcata tgaatccgga actggtcaat
    56701 cgcatgaagc agaccattcg ggccagacgg aagcgccatt ttaatgcaga acaccagcat
    56761 acgcgcaaaa aatcgatcga tctggaattt atcgtctggc aacgtctggc tggccttgcg
    56821 cagcgtcgcg gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac
    56881 aaagagaaat acgcgaataa aatgtcttct ttgaagcagg atctgcaggc attgctgggt
    56941 aaggaataac tgtcaaaagt cagttcaatt tactaaaggc gaaaaaaaaa ccccgcagct
    57001 gcggggtttt tctaccagac gagaacttaa gcctgcggct gagttacaac gtctttgatg
    57061 cctttaactt cgatctctac gcgacgatcc ggagccaggc aatcgatcag tgcagcacgc
    57121 tgtttcacgt tgtcacaggt gttgccagta accgggttgg attcgcccat accacgtgcg
    57181 gagattttgt ctgccgggat acctttagag atcaggtaat caacaacgga ctgagcacga
    57241 cgctcggaca gagcctggtt ataagcgtca gaaccgatgc ggtcagtgta acccagaaca
    57301 actacggaac cgtctttcgg atccaggttg ctcagctggc tgtacagctg atccagagca
    57361 gcctgacctt ccggtttcag ggttgctttg ttgaaggtga acagaacgtc agacttcaga
    57421 gtgaagtgct tggtctgtac ttccggtgcc ggagctggag ccggagcaac tactggagct
    57481 acttcgccct gaccgaaacg gtaggaaaca cccaggctca gcatgccgtt gtccggacga
    57541 gtgccgatgg tgtgtgcgtc accgatgttg ttggtccact ggtattccag acgggtagcg
    57601 atttcaggag tgatcgcata ctcaacaccg ccagcgaaga ccggagaaac gccggtgtcg
    57661 tggtttttac cataaacgtt ggatttagtg tctgcacgcc ataccatacc acccagacga
    57721 gtgtatacgt ccagatcgtc agtgattggg taacccagtt tagcggtcag ctgaacgccc
    57781 tgagctttat atgcaccgtt ttcaacgctg cctttgtatg gcatacgacc taaccagtca
    57841 taacccattt caaagccaac atacgggtta acctggtaac caccaaaagc accagcgccc
    57901 agttggtttt catgggtcgg accattgttg tcgatgaaac cagtgtcatg gtactgggac
    57961 cagcccagtt tagcaccagt gtaccaggtg ttatctttcg gagcggcctg cgctacggta
    58021 gcgaaaccag ccagtgccac tgcaatcgcg atagctgtct ttttcatttt ttgcgcctcg
    58081 ttatcatcca aaatacgcca tgaatatctc caacgagata acacggttaa atccttcacc
    58141 ggggatctgc tcaatattaa ctctaccgat atcttcggct tatgccgagc acccctggcg
    58201 atgtaaagtc tacaacgtag ttgaaaactt acaagtgtga actccgtcag gcatatgaaa
    58261 aaaaagtctt gtataaggta cgtttaatct tttttgtcag cgacaattta cagaagaaaa
    58321 tcgcggaaac cgcttcagac aagcctccgc aaggaaaatt agtcacgact gaaagcattg
    58381 gctgggcgac aaaaaaagtt ccaggattaa tcctaaattt acttaatgat acaaattaga
    58441 gtgaattttt agcccggaaa gttgtctcgt tgcgtgagag gatgcgctta ccggacgcat
    58501 aataaacccc atagcgttac cttcatttgc cgcatcaaca agttcagcat gctcttctgc
    58561 agtcaaatca tctgccaacc aaccgatcac cacgctgtaa ttgcccgtgc gtaaagcgcg
    58621 aaccattgac tccacggtgt ggcaagggga gagctggcta atctgcatta ctttcgttaa
    58681 gggtagccca gatgcctgaa cccattcgcg actcagtttt tgttgcggtg ttaaccagag
    58741 ttgccagcgc gggtaatgac tccaacttat tgatagtgtt ttatgttcag ataatgcccg
    58801 atgactttgt catgcagctc caccgatttt gagaacgaca gcgacttcc
//
LOCUS       DAZFFE010000017        58578 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFFE010000017 DAZFFE010000000
VERSION     DAZFFE010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.017"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(8..223)
                     /locus_tag="ACHJ69_001238"
     CDS             complement(8..223)
                     /locus_tag="ACHJ69_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO5014270.1"
                     /translation="MALNCPTCKTPLERNGETAHCATCDKDFTVQALCPECHQALQVL
                     KACGAVDYFCQHGHGLISKKRVEFIIA"
     gene            complement(286..1758)
                     /gene="der"
                     /locus_tag="ACHJ69_001239"
     CDS             complement(286..1758)
                     /gene="der"
                     /locus_tag="ACHJ69_001239"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO5014271.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYALGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDEFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            complement(1876..3054)
                     /gene="bamB"
                     /locus_tag="ACHJ69_001240"
     CDS             complement(1876..3054)
                     /gene="bamB"
                     /locus_tag="ACHJ69_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO5014272.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVNLAEKDG
                     WFSKDPALLSGGVTVSGGHVYIGTEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(3065..3685)
                     /gene="yfgM"
                     /locus_tag="ACHJ69_001241"
     CDS             complement(3065..3685)
                     /gene="yfgM"
                     /locus_tag="ACHJ69_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO5014273.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDAIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(3703..4977)
                     /gene="hisS"
                     /locus_tag="ACHJ69_001242"
     CDS             complement(3703..4977)
                     /gene="hisS"
                     /locus_tag="ACHJ69_001242"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO5014274.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(5088..6206)
                     /gene="ispG"
                     /locus_tag="ACHJ69_001243"
                     /gene_synonym="gcpE"
     CDS             complement(5088..6206)
                     /gene="ispG"
                     /locus_tag="ACHJ69_001243"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO5014275.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(6233..7240)
                     /gene="rodZ"
                     /locus_tag="ACHJ69_001244"
     CDS             complement(6233..7240)
                     /gene="rodZ"
                     /locus_tag="ACHJ69_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO5014276.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELNNNQSQ
                     SVPLDTSTTTDQAMATTPTSPVDTTATNTQTPAVTAPAPAVDPQQNAVVPPSQANVDT
                     AATPAPAATTTPDGAAPLPTDQAGVTTPAVDPNALVMNFTADCWLEVTDATGKKLFSG
                     MQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSPA
                     Q"
     gene            complement(7525..8679)
                     /locus_tag="ACHJ69_001245"
     CDS             complement(7525..8679)
                     /locus_tag="ACHJ69_001245"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO5014277.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(8829..9260)
                     /gene="ndk"
                     /locus_tag="ACHJ69_001246"
     CDS             complement(8829..9260)
                     /gene="ndk"
                     /locus_tag="ACHJ69_001246"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO5014278.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGSIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(9409..11721)
                     /gene="pbpC"
                     /locus_tag="ACHJ69_001247"
     CDS             complement(9409..11721)
                     /gene="pbpC"
                     /locus_tag="ACHJ69_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO5014279.1"
                     /translation="MPRLLTKRGCWIMLAAAPFIIILAAWAADKLWPLPLQEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSRERVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSVNLPEDPRPDSVSRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(11722..16683)
                     /gene="yfhM"
                     /locus_tag="ACHJ69_001248"
     CDS             complement(11722..16683)
                     /gene="yfhM"
                     /locus_tag="ACHJ69_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO5014280.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDDEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPVKLDVIKPDGQILRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     YEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGGGAVRPDRVTLKLDKASYCPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSNSPAPVELAPGVRTTLFIPVR
                     ALPGYGDGDIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAL
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKIMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDSALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TLQDLPGKWQAQTTFSAEPLTGEKAQTSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLPASNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAADDLLIVRP"
     gene            16890..17735
                     /gene="sseA"
                     /locus_tag="ACHJ69_001249"
     CDS             16890..17735
                     /gene="sseA"
                     /locus_tag="ACHJ69_001249"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO5014281.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPATRFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVTNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            18071..18196
                     /gene="timP"
                     /locus_tag="ACHJ69_001250"
     CDS             18071..18196
                     /gene="timP"
                     /locus_tag="ACHJ69_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO5014282.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNRSLSGNNLLVVNNLQNSK"
     gene            complement(18228..19004)
                     /gene="sseB"
                     /locus_tag="ACHJ69_001251"
     CDS             complement(18228..19004)
                     /gene="sseB"
                     /locus_tag="ACHJ69_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO5014283.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEGEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQAAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(19147..20430)
                     /gene="pepB"
                     /locus_tag="ACHJ69_001252"
     CDS             complement(19147..20430)
                     /gene="pepB"
                     /locus_tag="ACHJ69_001252"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO5014284.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGIRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(20489..20689)
                     /gene="iscX"
                     /locus_tag="ACHJ69_001253"
     CDS             complement(20489..20689)
                     /gene="iscX"
                     /locus_tag="ACHJ69_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO5014285.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(20701..21036)
                     /gene="fdx"
                     /locus_tag="ACHJ69_001254"
     CDS             complement(20701..21036)
                     /gene="fdx"
                     /locus_tag="ACHJ69_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO5014286.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(21038..22888)
                     /gene="hscA"
                     /locus_tag="ACHJ69_001255"
     CDS             complement(21038..22888)
                     /gene="hscA"
                     /locus_tag="ACHJ69_001255"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO5014287.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDAVSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQAIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(22905..23420)
                     /gene="hscB"
                     /locus_tag="ACHJ69_001256"
     CDS             complement(22905..23420)
                     /gene="hscB"
                     /locus_tag="ACHJ69_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO5014288.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(23516..23839)
                     /gene="iscA"
                     /locus_tag="ACHJ69_001257"
     CDS             complement(23516..23839)
                     /gene="iscA"
                     /locus_tag="ACHJ69_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO5014289.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(23856..24242)
                     /gene="iscU"
                     /locus_tag="ACHJ69_001258"
     CDS             complement(23856..24242)
                     /gene="iscU"
                     /locus_tag="ACHJ69_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO5014290.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(24270..25484)
                     /gene="iscS"
                     /locus_tag="ACHJ69_001259"
     CDS             complement(24270..25484)
                     /gene="iscS"
                     /locus_tag="ACHJ69_001259"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO5014291.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(25596..26084)
                     /gene="iscR"
                     /locus_tag="ACHJ69_001260"
     CDS             complement(25596..26084)
                     /gene="iscR"
                     /locus_tag="ACHJ69_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO5014292.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(26355..27095)
                     /gene="trmJ"
                     /locus_tag="ACHJ69_001261"
     CDS             complement(26355..27095)
                     /gene="trmJ"
                     /locus_tag="ACHJ69_001261"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO5014293.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            27214..28017
                     /gene="suhB"
                     /locus_tag="ACHJ69_001262"
     CDS             27214..28017
                     /gene="suhB"
                     /locus_tag="ACHJ69_001262"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO5014294.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            28162..29016
                     /locus_tag="ACHJ69_001263"
     CDS             28162..29016
                     /locus_tag="ACHJ69_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5014295.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            29207..30487
                     /gene="csiE"
                     /locus_tag="ACHJ69_001264"
                     /pseudo
     CDS             29207..30487
                     /gene="csiE"
                     /locus_tag="ACHJ69_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
     gene            complement(30479..31618)
                     /locus_tag="ACHJ69_001265"
     CDS             complement(30479..31618)
                     /locus_tag="ACHJ69_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO5014296.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGVGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVSMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            31987..32409
                     /locus_tag="ACHJ69_001266"
     CDS             31987..32409
                     /locus_tag="ACHJ69_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO5014297.1"
                     /translation="MNSLRYFDFGTARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAVIAVVMEVPAAILIILGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(32569..33419)
                     /locus_tag="ACHJ69_001267"
                     /pseudo
     CDS             complement(32569..33419)
                     /locus_tag="ACHJ69_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
     gene            complement(33430..34524)
                     /locus_tag="ACHJ69_001268"
     CDS             complement(33430..34524)
                     /locus_tag="ACHJ69_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO5014298.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(34557..35555)
                     /locus_tag="ACHJ69_001269"
     CDS             complement(34557..35555)
                     /locus_tag="ACHJ69_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5014299.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFDV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPIN
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(35580..37091)
                     /locus_tag="ACHJ69_001270"
     CDS             complement(35580..37091)
                     /locus_tag="ACHJ69_001270"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO5014300.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIMDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(37114..38097)
                     /locus_tag="ACHJ69_001271"
     CDS             complement(37114..38097)
                     /locus_tag="ACHJ69_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO5014301.1"
                     /translation="MPKKMTRTRNLLLMATLLGSALFARASDKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKTHVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFTQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(38194..41475)
                     /locus_tag="ACHJ69_001272"
                     /pseudo
     CDS             complement(38194..41475)
                     /locus_tag="ACHJ69_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311442.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            41593..42786
                     /locus_tag="ACHJ69_001273"
     CDS             41593..42786
                     /locus_tag="ACHJ69_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO5014302.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFERFQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWATPIEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(42850..44103)
                     /gene="glyA"
                     /locus_tag="ACHJ69_001274"
     CDS             complement(42850..44103)
                     /gene="glyA"
                     /locus_tag="ACHJ69_001274"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO5014303.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            44432..45622
                     /gene="hmpA"
                     /locus_tag="ACHJ69_001275"
     CDS             44432..45622
                     /gene="hmpA"
                     /locus_tag="ACHJ69_001275"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO5014304.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPNEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(45667..46005)
                     /gene="glnB"
                     /locus_tag="ACHJ69_001276"
     CDS             complement(45667..46005)
                     /gene="glnB"
                     /locus_tag="ACHJ69_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO5014305.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(46066..47400)
                     /gene="glrR"
                     /locus_tag="ACHJ69_001277"
     CDS             complement(46066..47400)
                     /gene="glrR"
                     /locus_tag="ACHJ69_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO5014306.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGVEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMTRGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(47390..48103)
                     /gene="qseG"
                     /locus_tag="ACHJ69_001278"
     CDS             complement(47390..48103)
                     /gene="qseG"
                     /locus_tag="ACHJ69_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO5014307.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGNWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTDEVTPDEP"
     gene            complement(48268..49695)
                     /gene="qseE"
                     /locus_tag="ACHJ69_001279"
     CDS             complement(48268..49695)
                     /gene="qseE"
                     /locus_tag="ACHJ69_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO5014308.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMSNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAKPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(50271..54158)
                     /gene="purL"
                     /locus_tag="ACHJ69_001280"
                     /gene_synonym="purI"
     CDS             complement(50271..54158)
                     /gene="purL"
                     /locus_tag="ACHJ69_001280"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO5014309.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHTIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLVDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVRRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFSEELGAVIQVRAADREAVEAVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEGFHVL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWIRIFRNARKQLG"
     gene            54416..55972
                     /gene="mltF"
                     /locus_tag="ACHJ69_001281"
     CDS             54416..55972
                     /gene="mltF"
                     /locus_tag="ACHJ69_001281"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO5014310.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKYPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(55969..56472)
                     /gene="tadA"
                     /locus_tag="ACHJ69_001282"
     CDS             complement(55969..56472)
                     /gene="tadA"
                     /locus_tag="ACHJ69_001282"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO5014311.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(56530..57165)
                     /gene="yfhb"
                     /locus_tag="ACHJ69_001283"
     CDS             complement(56530..57165)
                     /gene="yfhb"
                     /locus_tag="ACHJ69_001283"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO5014312.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            57374..58222
                     /locus_tag="ACHJ69_001284"
     CDS             57374..58222
                     /locus_tag="ACHJ69_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO5014313.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNTEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            58278..58538
                     /gene="yfhL"
                     /locus_tag="ACHJ69_001285"
     CDS             58278..58538
                     /gene="yfhL"
                     /locus_tag="ACHJ69_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO5014314.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGEHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
BASE COUNT        13877 a        16317 c        14581 g        13803 t
ORIGIN      
        1 catgatatta agcaataata aattcaacgc gttttttaga tattaaaccg tgcccatgtt
       61 ggcagaaata atccacagca ccacaagctt ttaacacttg taacgcctga tgacactcag
      121 ggcaaagtgc ctggacggta aagtctttgt cgcaagttgc acagtgagca gtctcgccat
      181 tgcgttcgag aggggttttg caggttggac agttgagtgc cataaatact cctcgcgtgt
      241 tattcggctt ccctctccgt gtggaaaggg aaaccagata agtgattact tacttttctt
      301 gatatgcttc atcagacgct tacgtttacg catctgggtt ggcgtcaggg tgttacgctt
      361 gttcgcatac gggttttccc cttctttgaa ctgaatacga atcggcgatc ccattacgtc
      421 cagcgatttg cggaagtagt tcatcaggta acgcttgtag gaatcaggca ggtctttcac
      481 ctgattgccg tgaatcacca caatcggcgg gttataaccc ccggcgtggg cgtatttcag
      541 cttcacacga cgaccgcgta ccagcggtgg ttggtgatct tcaacagcca tcgtcatgat
      601 gcgcgtcagc atagaggtcc ccacacgacg ggtggagctg tcatacgctt cacgtactga
      661 ttcaaacaag ttaccaacac cactgccgtg caaggcagag ataaagtgca cacgagcaaa
      721 atcgataaag cccagacgga agtccagcgt ctctttcacc tgctctttca cttcctggct
      781 caggccgtcc cacttgttca ctacaatcac aagtgagcgc ccactattga gaataaagcc
      841 cagcaaggag agatcctgat cggaaatacc ttcacgcgca tcaatcacca acatcaccac
      901 gttggcatct tcaatggcct gcaacgtttt gattaccgag aatttctcta ccgcatcggt
      961 gattttgccg cgtttacgta cgccagcggt gtcaatgagc acatactcac gtccgtcgcg
     1021 ttccattgga atgtagatac tgtcacgcgt cgtgccaggc atgtcgtaaa caacaacgcg
     1081 ctcttcacca agaatacggt tagtgagtgt agacttacct acgttcgggc gacccacaat
     1141 cgccagtttg atcggcagac tttgcgggtc gaactcgtct tcctcttctt cttcgccgtt
     1201 ctcttccgct tcaaattgcg cccagtattc ggcgtcttcg tcgacttctt cttgcggtgc
     1261 gagatcttcc atccacggca gcagcacgtg ctccagcaga cttaatacgc cacgaccgtg
     1321 agacgcggcg atcgggtaga tttcacctaa accaagcgcg tagaaatcaa ccactgcctg
     1381 atcgggatcc agaccgtcag ttttgtttgc caccaggaag gtcggttttt cacgggagcg
     1441 cagatgttta gcaatcgctt catccgccgg catcaggccc gcgcgcgcat ccaccataaa
     1501 cagtacgacg tccgcttctt caatcgccag cagcgactgt tccgccatgc gggtttctac
     1561 accgtcttct gtgccatcaa tcccgccggt atcaatgcag ataaactcac ggccttcaat
     1621 ttccgcacga ccgtacttac ggtcacgagt cagacccggg aaatccgcaa ccagcgcatc
     1681 tcgggtgcga gttagacggt taaataacgt ggattttcct acgttagggc gcccgacaag
     1741 cgcgaccaca ggtaccatgt ttaaagcctc atttttataa atcatcagac aacgcacgct
     1801 atattcgtgt cgttgttaaa aacaggaaaa cggcccctgt ccaggagccg ttttcaaagt
     1861 gaacgacaga gacgattaac gtgtaataga gtacacggtt ccgtcttttg cctggatcag
     1921 cagtttgccg tcagcagcaa ccggttcagt ctggaaaccg gaactatcaa ctttttgctg
     1981 ggcaacgaaa cgaccatctt cgacgttaat ccagtgcaga taaccttcac tgtcacctac
     2041 caccaggttg ccattataca gcaccggaga agtcagcagg cgatgcagca gatcgctttg
     2101 tgtccacagc gtaacgccgc catcaatggt caacgccatc acccggtcat tttgatcgac
     2161 cagatagatg cggttgccgt cgacgatgaa atcattcacc gaacccagtt cgcgtttcca
     2221 cataatctga ccgctgcgca gatcaagcgc cgtcaggtta ccattatagg ccagcgcgaa
     2281 aacaacgccg ttaacaacga cgggagtcgt gtcaacatcg ctcagacggt caatttcggt
     2341 agaaccggtc gcctgggaaa tacgctgctg ccaaatcatc tggccctgtt ccatcagcac
     2401 tgcgctgacg cgaccattat cgccacccac gacggccgca ccaaaagccg ttgccggcgc
     2461 agactcgcca cgcaaagaga gcgaaggcat atcgaggtta actgtccatt tgacagcgcc
     2521 gtcagcttcg ttcagcgctt gtaactgacc gttactggtg tggattaaca ccagaccgtc
     2581 gctgaccacc gggcgcgaaa gtgcttcacc cgcaacttta gtttgccatg ccacagtacc
     2641 atcgctggta ttcagcgcgt aaacctgcgc cttttcggtg ccaatgtaga catgcccacc
     2701 agacacggtc acgccgccag aaagtaatgc aggatcttta gagaaccagc catctttctc
     2761 ggccaggttg acagaccaga tttctttgcc gtcatccgca ttcagcgctt ttactaaacc
     2821 agcgcggtca gctgcataga caacgttgtc cgccagtgcc ggatgaagat tggaatagaa
     2881 gttgccaatg ccgctaccaa cggaagtgct ccacgcagta gtcggcgtaa actggttttc
     2941 aacggttggc aatggggaca tctttaccac atcttcttcg ctgttaaaca gcgaacagcc
     3001 gcttaaaagg gtaacggaaa gcagtcctgg cagcagtaat ttacgcaatt gcatcgggtc
     3061 cctctcagat ggacaaatta ttaattttca tctgcatcat ttcgctcagt gccggggtaa
     3121 catcgctttt cacgcctgct tcccatgcac tacgcgcacc ttgcttatca cctttgctca
     3181 gcaatgcttc accacgcagg tcggcaacaa tggcagccca cccttcacct ttgatagcat
     3241 caagggtttt tagcgcggca tcagcctgct tgagctgtac ctgaacgcga gcaagacgca
     3301 gatttatcac ggctttgaga ttttcatcgc tcgtgtctgc cagcccctgt tgtaactggg
     3361 cggcagcttt ctctagttca tttttgtcaa caaattgctg cgcaagttcc aaagaagcca
     3421 gcgcaccata agtattttta ttttcagcag caaatttttc cgccgctggg atgctatccg
     3481 gtttgccttc gctcactgcg gtaaccgcat tttgataggc aagagaagcg gagcgtgcag
     3541 aatcaacctg atggctgttc cagtagcgcc agccaatcag tgcgccaacg cccaaaatca
     3601 ccccaacagc cagtgctttg ccattttcag caaaaaagcg tttaaccgct tcaacctggt
     3661 cgttttcgtt ctcgtaaatt tccacgctgt ccttctcctt ccttaaccca gtaacgtgcg
     3721 caaatgcgcg gctacgctat cctgcgcaac tgcagtttgc tcaccagagc gcaaatcctt
     3781 cactactgct gtgccgttag ccacttcaga ctcacccagc accacagcaa cgcgggcacc
     3841 ccatttatca gcacgggcaa actgtttctt aaagttgccg ccgccgtggt tggtcatcag
     3901 tttcacgccc ggtaattcat cacgcagacg ctctgctaat gccatagccg cagattgtgt
     3961 gtcagcacct gaagccacca ggtatatatc gacaacagga tcggctttaa attccggatt
     4021 aacggcctga actaacaata caagacgttc gaggcccatc gcaaaaccga ctgccggtgt
     4081 tgcacgacca cccagttgtt ccaccagacc gtcataacga ccacctgcac acacggtgcc
     4141 ctgggaaccg aggctgttag tcacccactc gaaaacggta cggttatagt aatccagacc
     4201 gcgcaccaga cgctggttta cggtgtaagc gatccccgcg ctctcaagca gtttgcacag
     4261 accggcaaag tgctcacgag attcctcgtc cagataatcg cctaatgccg gagcgtcgtt
     4321 gagaagcgcc tgcacttccg gattttttga atccagtacg cgcaacgggt tagtgtacat
     4381 gcggcgtttg cagtcttcgt ccagcttttc tttatgctgc tcaaggaatg ccaccagcgc
     4441 atcgcggtaa ttcgcacgag cttccagcga accgatagag ttcagctcaa gagttacgtg
     4501 ctcggaaata cccagcgcgc gccaccagcg ggcagtgagc ataatcagtt cggcgtcgat
     4561 atccggacct tgcagaccga aaacttcgca gcccaactga tggaactgac gataacgccc
     4621 tttctgcgga cgctcgtgac ggaacatagg cccgatatac cacagacgct gttcctgatt
     4681 gtacagaaga ccatgctcga tgccggcgcg tacacagccc gccgtccctt cagggcgcag
     4741 agtcaggctg tcgccattgc gatcctcaaa ggtgtacatc tctttttcaa ccacgtcggt
     4801 gacttcacca atcgcacgtt tgaatagcgg ggtctgctct acaatcggca agcggatttc
     4861 actgtaaccg tagctgccga gcacgttttt cagtgtgcct tcaatgcgct gccagatggc
     4921 cgtttcgcca ggcaggtaat cgttcatgcc gcgaatggct tgaatgtttt ttgccacgtt
     4981 tattctcttt ctgaatataa aaatgaaccc tcaacgcttc cctcaatgtt tcgggagcca
     5041 tgcgggttca atcatacacg ggaagcgagg cgcttcccat cacgttatta tttttcaacc
     5101 tgctgaacgt caattcgacg cgcttcgtcc agctgactgg ctttcgcacg aatgcgcgct
     5161 tccagctgat cgatcatatc gttgttgtcc agacggtctt tgcgcacgcc atcttcatag
     5221 aggccgcttt tcttgttgcc gccagtgacg ccgagtgtag aaaccagcgc ctcaccaggg
     5281 ccattcacca cgcagccgat aatcgaaacg tccatcggag tgatgatatc ttccaggcgt
     5341 tgctccagcg cgttaaccgt accgataaca tcaaattcct gacgcgaaca ggtcgggcag
     5401 gcgatgaagt tgatcccgcg cgaacggata cgcagcgatt tcaaaatatc gaaaccgact
     5461 ttgatctctt cgaccggatc ggccgccagc gatacgcgca gcgtgtcgcc gatgccttca
     5521 gacagcagca gacctaaacc aatagcggat tttactgccc cgctacgcgc accaccggct
     5581 tcggtgatcc ccagatgcag cggctgatcg atttgttttg ccagcaaacg ataagactca
     5641 acagcgagga agacgtcaga cgctttcacg ctgactttga actgatcgaa gttcaggcga
     5701 tcgagatgat caacatgacg catggcagat tccagcaacg cctgcggcgt cggttcgcca
     5761 tacttttctt gcagatcttt ttccaacgat ccggcattaa cgccaatacg gatcggaatg
     5821 tttttatcgc gcgcacagtc aaccaccatg cgaatacgct cttcattacc gatattgcca
     5881 gggttaatac gcagacaatc gacgccgtat tccgctactt tcagcgcaat gcgatagtcg
     5941 aagtggatgt cagccaccag cggcacgtta acctgctgtt tgatgagttt gaacgcttct
     6001 gccgcgtcca tcgtcggtac ggagacacgg acgatatcag cgccaacacg ttccagcgcc
     6061 ttgatttgat tgaccgttgc ttcgacgtct gtcgtacgcg tattggtcat ggactgtacg
     6121 gcgatgggag caccatcgcc aatcggcaca ttcccaacgt aaatacgtgt tgattttcta
     6181 cgttgaattg gagcctggtt atgcatgaaa aatctcccgc gttacccgtc tgttactgcg
     6241 ccggtgattg ttcggcattg agggtcagac gcgcaacctg gttagttctg ataaaacgac
     6301 tcagatcgac aggtttccct tgatactgga tctgtactgc ggctggcgca ccaattttca
     6361 gtttgtacgg tgcctggccg gttaagttca aattaccgtc tttacgctgc ataccgctaa
     6421 acagtttttt accggtagca tcggtgactt ccagccagca atcggcagta aagttcatca
     6481 ccagcgcatt cgggtcaacc gccggcgtgg tcacgccagc ctgatcggtt ggcaacggcg
     6541 cagcgccatc tggtgttgtt gttgccgctg gtgccggagt cgcagcggta tcaacatttg
     6601 cctgcgaagg tggaacaacc gcattctgtt gcggatcaac agctggtgct ggcgcagtta
     6661 cggcaggtgt ttgcgtgttg gttgcggtgg tatccacagg tgatgtcggc gtggtcgcca
     6721 tagcctgatc tgtggttgtc gacgtatcta acggtacact ctggctttga ttattattca
     6781 gttccgcaga agattgatcg gccatagtgg tgatctcttc ctgctgagct ttgtggtctt
     6841 gccaccacca ggcaccgctc aggccaataa ccacaaacaa caccagccag gtgaaggtca
     6901 tcagccagcc gtcacgtttt ttgcggcgtt taccgaggga aaaactctgc atcggcgcaa
     6961 cttttgcagc ccgtagtgga gcctgctttt ccagccctgg cagcagttct tcttctggaa
     7021 tatgtaccag acgcgcataa gagcggatat atccgcgcag gaatgttgaa gcaagatcgg
     7081 cgggtgcctt atcttcttca atgtcgcgta ccgtggaaac cttcaggcaa agtcgctcgg
     7141 caacggcctg ttgactaagt cctagttgtt cgcgagcatt acgcaggcga gcgccggtag
     7201 taagtgcttc attttggtcg tgcgtggctt cagtattcat tcgctgcagg tacgtttaaa
     7261 tgagaattga gatgccggtg aaccatcatg cccacccaca ccgcgaaaca tccgttaagt
     7321 taaccgttat catacagtat aagaccgtct gtgtgctcgt gacaaagccc acattcgcta
     7381 aggctaactt actgttgcat cgttacatac tgccttaaag tcagcaaaaa cgcaccgtta
     7441 atattgacca gacaattgca acttaatact tcattcataa aagattacgc cacggtacat
     7501 aaagtaaccg tggcgtaatg gctatcagac cgctttaatg tcgatggctt caccctgcat
     7561 ccgcttacgc agagtacgtt tcgtacggtc gataacatcg cccgccaact gaccacaagc
     7621 agcatcgata tcatcaccac gagttttacg cacaatagtg gtgaaaccgt agctcatcag
     7681 cacttttgag aaacggtcga tacggctgtt cgaactgcgt ccatacggcg cgcccgggaa
     7741 cgggttccac gggatcaggt tgatcttaca cggcgtatct ttcagtagtt ccgccagttg
     7801 gtgcgcgtgt tcagtgccgt cgttaacgtg gtcaagcatc acgtattcaa tagtgactcg
     7861 gccctgattg gcgttggatt tctccagata acggcgcacc gccgcaagga acgtttcgat
     7921 attgtacttt ttgttgatcg gcacaatctc gtcacgaatt tcgtcgttcg gcgcgtgcag
     7981 ggagatagcc agcgcaacgt cgatcatatc gcccagttta tccagcgccg gaaccacacc
     8041 ggaagtggaa agcgtgacgc gacgttttga caggccaaaa ccgaaatcat caagcatgat
     8101 ttccatcgcc ggaacgacgt tgttcaggtt gagcagcggc tcgcccatac ccatcatcac
     8161 tacgttagtg attgggcgct gaccggtgac ttttgctgcg ccgacaattt tcgccgcacg
     8221 ccacacctgg ccgataattt ccgatacccg caggttgcgg ttaaagccct gctgggcggt
     8281 ggaacagaat ttacactcca gcgcacaccc cacctgcgaa gagacgcaga gcgtggcacg
     8341 gtcgtcttcc gggatatata ccgtttcaac gcgctgatcg ccaacggcga tcgcccattt
     8401 aatggtgccg tcagatgaac gctgttcttc aaccacttcc ggtgcacgga tttccgccac
     8461 ctctttcagt ttgccgcgca acactttatt gatgtcggtc atctcatcaa agttgtcgca
     8521 gcaatagtga tacatccact tcatcacctg atcggcgcgg aagggttttt cacctaaatc
     8581 tttaaaaaac tcccgcatct gctgacggtt gagatccagc aggttgattt ttccatcttt
     8641 cgtggtgacg ttttcaggtg tgactaattg ttcagacata tgctattccg gcctcgttat
     8701 tacacgttat ggcccctgga gggttgaaaa aagaaacgcc ccggtgagcg gctgctcgtc
     8761 cgggggcgct gcattgtaca aattctggcg cacggatgcc acgtttgcac gcggcattta
     8821 cgaaattatt aacgggtgcg cgggcacact tcgccttcgc cgaagaaata agcgatttcg
     8881 cgagcggcag attcgacgga atcagaaccg tgggtaccat tttcggtcag gctgtcagcg
     8941 taatcagcac gcagagtacc ggccagtgcg tttgccggat tggtcgcgcc cagcagatcg
     9001 cggtgacgct gaacagcatt ttcaccttcc agcacggaaa caacgatcgg gccagaagtc
     9061 atgaattcaa ccagaccatc aaagaacggt tttccatcgt gttcagcata aaagccacga
     9121 gcctgttcaa cggtcaggtg cagcattttg gtgccgacaa ttttgaaccc tgcagcttca
     9181 aagcgcgcaa agatactacc aatcacgttt tttgctaccg cgttcggttt gatgatggaa
     9241 aaagtacgtt caatagccat ttttacctct gtaaattgtt ctgttgttgt ctgtaccagc
     9301 gtacgagatg gcgcggatta taatgagcaa cagggccgtt gactattgat gaaggtaaca
     9361 tttttttaaa ataaacccag tttaagcaac aatcagggac aaaacagact attgcatgac
     9421 aaatttcact gtcgctattt gccccacgtc atccatcacc agcaattgat aatcgccttt
     9481 atccgtcaaa tgcagggtca cgttgcgccc acgttcagtt aacggttcgc cgttcagaaa
     9541 ccaccagcgt tcacctgccc ctccacttga ttgcaacggc aaagtcgctt ccgctgcgcc
     9601 cggtaaacgt ttaataatcg cgccatcgcg cacacctgtt agctgcagcg ggagttgtgc
     9661 atcgtgaccg tatggcgggc aaattgtcga ggccggtggc aagcgcacag cgcgacgttc
     9721 tgatgcgggc agccagggtt ccagcggtag cggccagaca tttatcattt cttgtcgcgc
     9781 ctgagggcaa tcagcggcaa cacgtttgcc gttttcatcc agccagatgg ggaaacgaat
     9841 gccattaatg ccttcctgct ccggcagtaa tagagttggc ggctggctcc cgtccagcag
     9901 ccaggttgcc aggcggcggc gacagttacc gtcaccttcc ggcaaagact gtccgcccgg
     9961 ccagcagata acgccacgac ttacagagtc aggacgtggg tcttccggca aattcacact
    10021 gcgcgacagt aagatattat taacctgatt caacagcggt acggcactgg caaagccaaa
    10081 ctgaccaaca acgggcgtgc cgtccggtct gccagtccag atcccaatga catagcgagc
    10141 gttaacccca atcgcccagg catcacgata gccatagctg gtgcccgttt tccatgccag
    10201 tggggcgacg cgcggcaagg caccatccgg caagggttgc gcttcatcag ccataatccg
    10261 gcgaatgatc cacgcagccc ccgacgacat taaaggccgt tcaagcagcg gatcgtcagg
    10321 ctgtaagcgc aatttacctg ccttgccgtg gcgagcaaac gcggtatacg ctgccgccat
    10381 atcttccagt tttgcgccag cgccgccgag aatgagcgaa agattcggtg cagcaccgtt
    10441 gggcaaatat aacggcaatc caacattgcg taactttgcc gcaaaccgtt tcggtccata
    10501 ggcttccagc acctgcacag caggtaagtt cagcgatcgc accagcgcct cactcatgct
    10561 gatcgggcca tgaaaaccgc tgtcaaagtt gccaggtcgg taatcaccgg tgcgtctggg
    10621 gacgtcttgc aacagcgatg ccgggtggat caagccttca tccagcgcca gaccataaac
    10681 aaacggtttg agcaccgatc ctggcgatcg gatcgcattg accatatcaa catgaccaaa
    10741 gcgtgaatca tcgttgagat caaccgatcc cacccagccg cgaacacgca tatcggtatg
    10801 atcaaccacg atcatcgcca gtgagctgcg cggtggcaat cgtcctttcc agttttgcgc
    10861 cagttcttcc agacgtcgtt gaagaccggc atccagcgta gtaacgattt tgtcgctttt
    10921 gcttttaccg agcatcatgc gcgaaaacag cggtgccagt tgcggcattt gtcggggtgc
    10981 cagccagatg ggttcttccc ttgactcttt tacccgctca cgggaccaca caccttgcac
    11041 ggccatccgt tcgagcactt tattacgcgc ggcttcggca cgctccggcc aacgatccgg
    11101 gcgaagacgg ctgggcactt gcggcaacac cgccagcatt gccgcctcgg aatagcttaa
    11161 attcgcaggc gatttcccga ggtaagccca acttgccgca ccaatcccct gcaacgtacc
    11221 accaaacggg gcacggttaa gatacaaggt cagaatttca cgtttagaca gatgccattc
    11281 cagttgcaac gcgcgccaga gctggcgaat tttgccgcca aacgttttgg ggtgaggatc
    11341 aagcaggcgc gccacctgca tggtgagcgt gctgccgccg gaaatgaccc gacccgacgt
    11401 gagatcttgc caggctgcac gcgccaccga gaacggattc acccccggat gcttccagaa
    11461 ccagcgatct tcataattga tcagcgcttc aaggtaacgt ggagaaacat cttcgattgt
    11521 tactggataa cgccagatgc cgtcagcatc ggcgaaacgc cagagtggcg taccatcctg
    11581 cgccaccacc acgcgtgcag gattcacttc ttgcaatggc agaggccaga gtttatctgc
    11641 tgcccatgct gccagaatga tgataaaagg cgcggctgcc agcattatcc agcagccgcg
    11701 tttggttaac aggcgaggca tttacggtct gactatcaac agatcgtcag ccgcaccagt
    11761 cgcccgccat tgaggaacat acattgattc caccataggc tgcggcacct gatacgttcc
    11821 cggcgtcacc gcccgcgcca gatacaccag cgttaccggt tgatattcat caacggcaac
    11881 cgccgccaca aagcgatcgt cacggaactc tatgtgctta atgctcgcct gctgcatctg
    11941 gttcagcaag ttctgcactt caccaccact ttgctccagg ctggcgctgc cgttcgccag
    12001 attctggttt tccagttcca gccccgcagg caacagatcc acgactaacg catccggtac
    12061 actgttactg gctttaacct gcaaccacac cagcaccaga tcgccgctac gtaacgagtc
    12121 cagcgatttg ctcttaccgt cagtacccag aatatgacgc tcaatttgca gcacattgct
    12181 cgccggtaaa ggtgcggatt gcggataacc gctggcatcc acacgcagcc ataacggctg
    12241 atcgccactg ttggtcacct gcaaggtggc aagttgatcg ctattcagat tgctggtttg
    12301 cgctttctcg cctgtcaacg gctcagcaga gaaagtggtt tgcgcctgcc atttgcccgg
    12361 taaatcctga agagtacggg cagccaggaa caacgcgtta ctttcctgcg tcgatagcca
    12421 gcgttcaccg aacgcctgct gcgaaagtgt gtttaacagg gagtactgct catcgggtag
    12481 cagtttgttc tcttccagca atgagagcat caacgcgctg tctcgcagtg gactaccgta
    12541 atcacccagc catatccgct catcactatt acgcggcgtt ttcagcgcca gcgcaatcgc
    12601 ttcttcacca cgcgtcgcat cacccatgat tttcagcgca acgccaagtt gcagcagcgg
    12661 taaaccagaa gcggcatctg cacgatgctc ccagatttca cgcagcgcac ccagtggtgc
    12721 tttttgctga cgggccaaca ccagcgcagc gtaagactgt acggcgaatt tactggcttt
    12781 gagattatca gcgtacggga tcgacatcat gcccggatct tgtaaatagc gcagcagacg
    12841 ctcattaccc cggttaatgg cgtctgtcgg cacgctgtaa ccctgttcgc ctgcgcggac
    12901 caggaaatcc atcacgtaag ccgtcagcca gtactcttcg tcaccgtttt tatcccacaa
    12961 cgcaaagccg ccgttatcac gttgcatttg cagcaaacgg gaaatgccga tatcgaccga
    13021 tgcacggcgt ttctcatcac tgtcgccttt gatgcccaac gcctgcagtt gggctgcatt
    13081 ggtataaagt gacgggaaca ggccgctggc ggtttgctcg agacagccgt acggatacgc
    13141 ttttaactct ttgatataac gtgcgatgtt cagcggtggt ttgccactca acaacaattg
    13201 cccttccagc gtaacaggcg agaagttttg caatccatca gccgggagcg cccaggtttc
    13261 accaggctgt aacgccgtac cgtaattgac cgtttgtgcc gggaatgccg gacggacgcc
    13321 gattttccac tgcttatgct gatcggcaac ggtttccccc ggtaacgcta acccgctaat
    13381 ggtggcctga atgtctccat cgccataacc cggcaatgca cgcaccggga taaacagcgt
    13441 agtacgcacc cctggcgcta attcaacagg tgcgggggaa ttactcacca gttcaagcaa
    13501 gccactggcg gtcagagcaa cattaagttt ttgcggttta tcggtaagat tagtgatatc
    13561 cagcgtcagg cgcgaggtat cgccactcgc cataaagcgc ggcatgttca gttcagcaat
    13621 caccggtgcg gcaacaatca ctttgctttc gttgcttccg aagtcatctg ccgtccaggc
    13681 ttgcgccatg acgcgcaatt caccgttaaa atcgccaatt ggcagtgtaa ccgagccttc
    13741 gccctgttca ttgagcgtta ccggcagcgc ctgctgcgcg acaatattga cgtgattgac
    13801 cggcggttta ccaccacgtt tcagctcatc accatcgcca ccaaaacgca gagctgccag
    13861 acgcccctga ccttcaatga cctgaccgta aatatcgtaa atgtctgcgc catagcgttt
    13921 ctgaccaaag aacgcctgcc acggatctgg cgtgacgtaa tcagtaatat tcagcacacc
    13981 gctatcaacc gcagacacca gcacattcac ctgttttggc atctcaccat ttttattgct
    14041 ggctttaatt ttcacggtta atggctgatt ggggcgcatt ttggtggggg tttccagcgc
    14101 cagatcgagg cgacggtttt catcaccaag cggcagatgc aacaccccaa ccgcgcgttt
    14161 tggcgtcgcg gagcgagatt tatcgccagg acgtaccacc agcgtactta aatacagatc
    14221 atgacgattc caggttttat cgaccggaat cgtcagatcc agcccttgag ccggaacatc
    14281 aatctcttgc caccacagcg gcccttcact ggactcgacc atcgcataac ctttacccgc
    14341 cgttggcgcg gcaatatgca acttaatggt atcgccaggg caatagctgg ctttatccag
    14401 tttcagcgtg acacggtcgg gtcgcactgc accacccccg tcgctgttat cctgccagct
    14461 atacccagcc cagaaacgca cactactgac cgcttcattc ggcgctttaa cttccagacg
    14521 ataagcgccc cactctaccg ggaaactgac cttaccggtt tcgtccgctt tcagatccag
    14581 agtttgttca ttttcgatca gatctttttg atcaaactgt gactgccagc cttcatattc
    14641 cgaccagttc cagtagtaat cgcggcgttc gcgaatcaag cgcacctgca agcccgacac
    14701 ggcttttttc acgccttgcg catcgctata aacgatgtca aaagcggcgt tactaccttc
    14761 atcaacaatg ggctgtttca ccgtgctgtc ggtgcgataa tcgtaaaccg atttcgaggc
    14821 gaactgcgga cggatccctg gcaatgcatc agcaggccag atagcctgct cagcacggcg
    14881 cgtcaccggg cgaccgcccg attccagcaa actaccctgg aaaataacct gtaatgggga
    14941 atgtgtttcc ttccactggc tttctgtaga aacttcgccg cgccctttat catccagcgt
    15001 caactgaact tcatccagcg tgcgggaaag attttcggca gctatatcgc cgaattcaaa
    15061 accaggtaag gctgacacag cttcacgcag tgggcgcagg aaaagttgcc cttgcaaagt
    15121 attaccattc gcaggtgcac catacaggta gtaccccacc acggagaatt tcacttcatc
    15181 tttcggcgtt agcggggttt tctcaccggt cagattcagc gccatacgct ctggcataaa
    15241 atcttcgacg tggaaatccc acatccgata ctggttatcg cccgtgttag cgcgaatatg
    15301 ccacataccg gttgccgcat tgctatcgag tggccaggta aagtggtaga ggccattctc
    15361 cggctgacta acgacgctcc tgagtatctg cccgtcgggt ttaatcacgt ccagcttgac
    15421 aggttgatcg ggcaacgctt taccgtctgc atcacgcagc aaaccattga ggatgaccgt
    15481 ttcaccaggg cgataaagat cgcgcgggcc aaacatgaaa aactgtttgc tatagcctgg
    15541 cgcgccagca atgttaaatt ctgctaagtc cagcgccgga agttttaaat cgagtagcgt
    15601 tgtctgaccg tctttacgcg ccagcagtaa tgccgcgttt ttatcatttt ccagctgcac
    15661 atgcccctga gcgtcactgg ttgcctgagt cagagtctgc cctttctcat ttaataaaga
    15721 gacttctatt ccttgctggg ccgcgccgtt ttccagactt tgggtaaaga tatccagacg
    15781 attgtgataa cggtgagctg aaacgccgat atcacttaac gtaaacagcg tcgcgggatt
    15841 actgtaatcg taacgtccag cctgattcat cacagccaga tacacgcccg cctgctgaag
    15901 cggtttgata tcgcccagcg gcagcaataa tttttcacgg gtgttacgcg caggattgag
    15961 atcaaaccgt ccggtgtaga ccagatccgc catctgcagc agtttatctg actgccagtt
    16021 cgccagcgaa ttgcggtatt cccactggct aatgaaagcg ggcagtgatt ctggtttcac
    16081 acggaagaag ttaacatcga cattgttaac gttaagcgcc attaccggca gcccttcaac
    16141 gaccttacca ggaagcaacg aaccacgact ggcaaaaccg acgctgggtt ggatatcgcg
    16201 ggtagttata gttttttcgt catctttact aaaggttgcg ttattgagcg ctttgacctc
    16261 cttgccaata gtgacgatca aatcacgttt cggttcgagg tggcgtaaac gcagctcttt
    16321 aagattatct gacagctccc aggcaccatc cactttgccg ctttttttat cgacgacatg
    16381 aataacgcgt gagaaatcct gatccgggtc gagagggatg gagaacgtca gcaccagcgt
    16441 ggctgcgcca tcgagttgaa cttcagagag atccagcaat gtcagcgcct taccggcact
    16501 ctgttgggcc agtttttgtc gctccggcgc ggaaagcttt gctgaactca cgttttctga
    16561 agaggccgct ttagtaactt cagaaggcgc atcttttttc accgctgttg gcgcgttatc
    16621 gttgttgtcg caccctgcca gcgccagcat tagcatgcag gcggctacgc gtaacttttt
    16681 catttttcat ccctgccaca atggcccgtt agcaacgtcg aataattatt atgcgtgaga
    16741 atttacgtta tgtaattcag tatcacgccc atacaatccg aattttagtg agaattgtgg
    16801 ctcagacagc caaatcagcc tcttttgctt gtcgcttttc actaaacatc cgacaattta
    16861 gcctaacccc ggcaaaaatg gagatgccta tgtccacgac atggtttgta ggagccgact
    16921 ggctcgccga acatattgat gacccggaaa ttcagattat cgatgcccgc atggcgtcgc
    16981 ctggacagga agatcgtaac gttgctcagg agtatctgaa tggacatatt cccggcgcag
    17041 tgttttttga tatcgaagcg ctttctgatc acacttctcc gcttccgcac atgctgccgc
    17101 gcccggaaac gttcgccgtg gcgatgcgtg aattaggcgt taaccaggat aagcacctga
    17161 ttgtctatga cgaaggtaat cttttctcag ccccacgagc atggtggatg ctgcgcacct
    17221 ttggtgtaga gaaagtgtcg attctgggtg gtggacttgc aggctggcag cgtgatgatc
    17281 tgctattaga ggaaggcgca gtagagctgc cggaaggaga gtttaacgcc gcgtttaatc
    17341 ctgaagccgt ggtgaaagta accgatgtat tattggcaag ccatgaaaat acggcgcaaa
    17401 ttattgatgc ccgcccggct acacgtttta acgcagaagt tgatgaacct cgcccaggtt
    17461 tacgtcgcgg acatattccc ggtgcattga acgttccgtg gacggaactg gtacgtgaag
    17521 gcgaactaaa aacgaccgat gaactggatg cgatattttt tggtcgcggt gtcagctacg
    17581 acaaaccaat tatcgtcagc tgcggttctg gtgtaacagc agccgtggtt ttgttggcac
    17641 ttgcgacgct ggatgtgaca aacgtgaaac tgtacgacgg cgcatggagt gaatggggcg
    17701 cacgggcaga tttaccggtt gagcccgtaa aataagcttt ttacaggcaa taaaaaaccg
    17761 ccgaatttgg cggtttttta ttgctggtct ggttcgcggc ctttccagca ggttgtatta
    17821 ccgtagtaat gcaagcgcgt ctcagcggac acaatactcg ccagtaactc tctttttgtc
    17881 aagtaaaaga gagttattat tgttctgtta gtgtattatc cactgcggcc ctttccgccg
    17941 tctcgcaagc gggcgctggc ttaaggaaag gatgttccgc gaccgttagg gcaagcgcgt
    18001 ctcagcgctt gctatcgcgg caatatcgcc agtggtgctg tcgtgatgcg gtcttcgcat
    18061 ggaccgcaca atgaagatac ggtgcttttg tatcgtactt attgtttctg gtgcgctgtt
    18121 aaccgaggta aataataacc ggagtctctc cggcaacaat ttactggtgg ttaacaacct
    18181 tcagaacagc aagtaagccc gaatgccgcc ctttggggcg gcatgtttta aattatccgg
    18241 ttctgtttaa agtcacgcaa aaaaccaccc cagcgacgtt catagaatgg cgcaatatgt
    18301 tcggtaataa agtggctaat acctttttcc ccttttttca cctgacaaat atcgatgggt
    18361 tcatcgccag gtaatgtatc ggtcgctaca cttcccgccg cctgaataat ttcttcgata
    18421 tcaccatcgg cttcaatgcc aataagtaaa ttaggctgtg cctcttcgtt ctctttaatt
    18481 gaacaaataa aagcacgctt caccggctta atggttttaa ataaggtggt aagtgaatca
    18541 atcatttgcg ctggcggctc tgcgacctcc gataatatca gcgattcacc gccttccagt
    18601 acttcctgac tgctaagcgg attcccttct tcaccaatca gcaaactgat ttcacgcggc
    18661 ataaattctt taccggttgg cagtttggca ttaaggaaga gcgtttcgcc aagggtcatc
    18721 tcaaacaatg tgcgaactgg cattacgaca aatgcctgtt cgccttcaac cgcctgttga
    18781 agtgcttcta acgaggtgaa aaaaggaatg acgctggtgc cgtcttcttt ttcccagtgc
    18841 tgtaaatcaa gtgcgctatc ttcaaccaca gcctcgccct gtgccgccgt accaggcacc
    18901 cagacggtgg attccagtag agtacggaaa aaggccgggc ggtgcgccgg ttcggttgct
    18961 gctttttcca gcaggtcttc aagttcgttt tttgtttcgg acataagaac cataattcat
    19021 tcaacgttcg gggcgtaagt gcctgatgcg ctacgcttat caggcctaca agggaaccgc
    19081 aatatattga atttgcaata ctttgtaggc cggataaggc atttacgccg catccggcat
    19141 attagtttac gccgttaaca gattagcaat cgtgcgcaca ccaagtcccg tagcacccgc
    19201 agaccactgt tcaaccggcg ctttacggta agtcgccgag cagtcgatat gcagccaacc
    19261 ttgctgatag ttctcaacaa agtgcgacag gaagcccgcc gccgtgcttg cacctgccgg
    19321 atacgccgcg cttccggtat tgttcagttc cgcaaagtta gacggcagct ggctgcggtg
    19381 gaactccgcc agcggcagac gccagaacgg ttcattctcc tgcgcggcac tcgccagcaa
    19441 gcgccctgcc agcgcatcat caaaactgaa cagcgcgtga taatcattac ccagcgcggt
    19501 tttcgccgcc ccggtgaggg tcgccgcatc aataatcagt tccggtttct gcgcactggc
    19561 atcaatcagg ccatcggcca gcaccagacg cccttcggca tcagtgttca tcacttcaac
    19621 ttttttaccg ttgcgatagg taataatatc gcccagtttg aacgcgttgc cgctgatcag
    19681 gttatccgca caacagagga acagcttcac gcgcttgttc agtccgcgcg taatggcaaa
    19741 tgccagcgcc ccggtaaccg ttgccgcgcc gcccatgtcc gacttcatcg agtccataaa
    19801 cgcagtctgt ttgatgctgt agccgccgga atcaaaagtg atgcctttac caaccaggca
    19861 cgcgtacact ggcgcttctt tatcgccagt tgggttgtaa tccagcgcca gcaataccgg
    19921 agaacgttct gaaccgcgcc cgaccgtgtg cagccccata taaccttgct cacgcagatc
    19981 ttcgcctttg gtgatccggt aagtcacacg atcccctgcg acgttgctga tcagatcaac
    20041 agcacgctgt gccagttgcg atggccccag ctcttctgcc ggtgcgttga tggtgtcacg
    20101 cacccagtcg atgatcatca ggcggttatc cagttcctga cgctgggcat cgtccagatc
    20161 cggccactcc actttacgta tgcctttcgg ggctttgtaa ccttgccaga atgcccagca
    20221 gcgatccgca tcccagcctt caccgcttaa ctgaacatgc ttgatgccca gaccgtcaat
    20281 cttgcgcgcc gcacgctgga tcaaccccag atcgtctgcc ccgttcaaat gcagggtaat
    20341 gccatcatta ttaatgctgt aagtcgcttt ttctccccaa cgtgcgtcgg caggttgggt
    20401 cgagagggta atcttcatcg cttctgtcat tttagttatc cttattcgtt taacagcaaa
    20461 acgggctacc cccaggcagc ccgttcattt actcggcctc gtcaagccag accaacaaaa
    20521 tcgcttcgag gattttctcg ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc
    20581 aaatccactg atgcatatcg gtgaatcgaa ccgttttcgg atcaagatcg ggatacgcat
    20641 cgtacagtgc ttcgccaatt tcgcggctat ccgtccactt aagtcccata ctaacctctg
    20701 ttaatgctca cgcgcatggt tgatagtgta acgcgggatt tcgaccacta aatcttcgtc
    20761 ggtaacgcgc gcctggcagc ttaaacggct ttccggctcc agtccccagg ctttgtccag
    20821 catgtcgtct tcctgctctg agctttccgg cagcgagtca aaaccttcac gaacgatgca
    20881 gtggcaggtg gtgcaagcac aggatttttc acaggcgtgt tcaatctcga taccgttacg
    20941 cagcgcaacg tcgagaatgg tttcaccgct attcgcttcc agaacagcgc catcagggca
    21001 gagatcctga tgaggcaaaa taacaatctt tggcatatta aacctcgtcc acggaatggc
    21061 ctttcagagc acgacgaacc gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt
    21121 tgtctacgtt tttaatcgct tgttcgatgg cgtcaacatc atcgccctgc gccacttcac
    21181 tcaggtgagc ggcagcatcg tcgatagcct gacgttctgc ggcgcttaac agcgcggcat
    21241 cagcagccag cgcaccgtgc agactttcca gcacacgcgc cgcttctact ttttgttctg
    21301 ccagcattcg ggcttttacg tcctgctcgg catagctcat tgagtctttg atcatcgagg
    21361 cgatttcgct atcggtcagg ccataagacg gtttgacctg aatagacgcc tcaacgccgg
    21421 tggatttctc catcgccgtc acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc
    21481 gaatatgcgc accgccagcc ggtagcgccg gaatacctcg cagcgcaaaa cgcgccagcg
    21541 agcggcagtc ctgcaccagt tcgcgctcac cttgcatcac atggatagac atcgccgtct
    21601 gaccatcttt aaaggtggtg aaatcctggg cgcgggccac cggaatcgtg gtattacgcg
    21661 ggatcacttt ctccaccagg ccgcccatcg tttcgaggcc cagcgacagc gggatcacat
    21721 ccagcaacag catttcgctg tccggcttgt tacccaccag aatatccgcc tgaatcgccg
    21781 cgccaatagc gacgacttta tccgggtcga tagaagtcag cggtggacga ccgaaaaatt
    21841 cgcctactcg ttcacgcacc agcggcacgc gagtagaacc gcccaccatc accacttcca
    21901 gcacttcatc agcttctacg cccgcatctt tcagcgcgcg acgacaagcc agtaaggttc
    21961 gttttaccag tggcgcgatc agttcattga attgttcacg gctgatttcg ccctgccagc
    22021 ccgcaacgtt aacggtcacg gagaccgcat cgctcagcgc gattttggcc gcaatggcag
    22081 catcgagcag ttcacgctga acgcggttat cgctacgatc aggaatgccc gcctgctcgc
    22141 gaatgtaatc cgccagcaga tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg
    22201 tcgccagcac ttcaaacacg ccgcgactta agcgcagaat ggaaatatca aacgtcccgc
    22261 cgccgaggtc ataaacagcg atcacgcctt cctggccgga atccagcccg taggcaatag
    22321 ccgcagcggt cggttcatta agtaagcgca gaacatgcag ccccgccaga cgcgccgcgt
    22381 ctttggtgcc ctgacgctgg gcatcgtcaa agtacgctgg aacggtgata acgacaccat
    22441 ccagctcgcc tgccagggct tcagttgccc gcgccgccag tgctttgagg atgtccgcag
    22501 aaacgcgcac cgggttcagc agccccgccg ccgtttcaat catcggcagg ccattttcgc
    22561 tggcctggaa ttgataaggc agatgcggat agcgttgctg gatatcagcc agcgagcgcc
    22621 ccatcaggcg tttaacagaa ctaatggtgt tggcggtgtc gagcgctgcg ttagtacgcg
    22681 cgtcataacc caccgaatgc ccttgctgtt gatagtgaac aacagatggc agcaggtgac
    22741 ggccttcatg gtcagctaac gtttcggcct gaccgctgcg cactgttgcc accagcgagt
    22801 tggttgtgcc caggtcaata ccggccgcca ggcgacgctg atgcggcgca gcactcaaac
    22861 caggttcact aatttgtaat aaggccatgt ttagcttcca gaaattaaaa atcgagcagt
    22921 ttttcttcga gttgttcggc actgctccgc agtttatcga gaaaacgcag cttacgcacg
    22981 gtatccgccg ccgcgtccca cgtctcgttg tctaactgtt caaccatcaa ctgatggcgg
    23041 gtatcaaaca tctttttcac acgtttgata aagctttcca gccgcgcttc atctttcgcc
    23101 tgttcgatct cgtccagttc ttcgcgcagt tccagctgtt ccatcaggaa cgcggtgtcg
    23161 cgcacagtat gctgctcgct ggcgagatca aaaccgtgca aagaaagcaa atattccgcg
    23221 cgcattaacg gatgacgcag cgtttgccag gcctggttaa tggtcgcgga ttgctgtacg
    23281 gcggcgagtt gttccgcctg gcttccgctg gcgaatttat caggatgata ctgacgttgt
    23341 agatcctgaa aacgcaggct cagcgcctgg gtatcgagtt gataacgggc aggcaagcca
    23401 aagagggtga agtaatccat aacattctca gggctgcgat ccgccgcgat aacggcgaat
    23461 caggtaaaac aaaccccacg cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa
    23521 cgtggaagct ttcgccgcaa ccacactcat ctttgacgtt cgggttggtg aatttaaacc
    23581 cttcgttaag gccttctttg acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt
    23641 tgccatcgac cacgactttc acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt
    23701 cgtcaacaaa ttccagtaca taagccatac ctgaacaccc ggaggttctc acgcccagac
    23761 gcaggccaaa ccctttaccg cggttagcca gaaaggtatt tactcgcgct gctgcactgt
    23821 cgctcagtgt aatcgacata accaaacctc aactcttatt ttgcttcacg tttgctttta
    23881 tagtccgcaa tggcggcttt gatcgcgtct tctgccagaa tagaacagtg aattttcacc
    23941 ggcggcagtt caagttcttc agcaatatcg gtgtttttga tcgcctgtgc ttcgtcgaga
    24001 gacttccctt tcacccattc ggtgaccagg gagctggaag cgatagcgga accgcagccg
    24061 taagttttaa aacgcgcgtc ttcaatgata ccttcatcgt tgactttaat ctgcaacttc
    24121 atcacgtcgc cacaggccgg tgcacccacc atgccgctgc cgacgttctc gtcgttgttg
    24181 tcaaaggaac ccacgttacg cggattctcg taatggtcga taactttttc gctgtaagcc
    24241 attataaatt ctcctgattc cgataccgat taatgatgag cccattcgat gctgttcaga
    24301 tccacgccct gcttgtacat ttcccacagc ggagaaaggt cacgcagacg accgatggat
    24361 ttacgaacta actcgatggt gtagtcgatc tcttcttcag tagtaaaacg acctaaagag
    24421 aaacggatag agctatgtgc cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac
    24481 ggttcgaggc ttgctgacgt acaggcggaa cctgaagaaa ccgcgaggtc tttcagcgcc
    24541 ataatcagcg actcaccttc aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg
    24601 tgttccaggt caccgttcag gtaaacttct tcgatatctt tgatgccgtt ccacagacgg
    24661 ttacgcaggc cgcgcagacg ttccatctcg gtcgccatct cttcttttgc gatgcgatag
    24721 gcttcgccca taccgacgat ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg
    24781 tgaccgccgc catgcatttg tgcttcgata cgtacgcgcg gtttacgacg cacatacagc
    24841 gcaccaatac ctttcgggcc atagattttg tgaccggaga aagacatcag gtcaactttc
    24901 aactggctca gatcgatcgg cagtttgccc acgctctggg ttgcatcaac gtgatagata
    24961 atgccacgag cacggcacat ttcgccgata gccgcgatat cctgcaccac gccgatttcg
    25021 ttatttacgt gcatgatgga aacaaggatg gtgtcgtcac gcatcgctgc ttcgagttct
    25081 ttcaggtcga taatgccatt acgctgcggt gccaggtaag tgacttcaaa accttcgcgc
    25141 tccagctgac ggcaggtatc cagtaccgct ttatgttcgg ttttactggt gatgatgtgc
    25201 ttgccttttt tctgataaaa gttggctgca cctttgatcg ccaggttgtc agattcggtt
    25261 gcaccagagg taaagacgat ttcacgcgga tcagcgccga ccagatcggc aatctgatta
    25321 cgggcgatat ctaccgcttc ttcagcctgc cagccgaaac ggtgagaacg ggaggccggg
    25381 ttaccaaagg ttccgtccat cgtcataaac tgcatcattt tctcggcaac acgcgggtcc
    25441 accggcgtgg ttgcggagta gtcgagataa atcggtaatt tcattgctct ataaactccg
    25501 tacatcactc aatgcaagga accaggctac cggttggatg tacgaccgtg tttacggagt
    25561 gtttagcact ccggcctgat tctgaattct ttttattaag cgcgtaactt aacgtcgatc
    25621 gcgtcttgtg tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga cacatccagc
    25681 acttcctggt tattaaccag ttcgcctaaa gtaatgttat tgagaaaacc ggtaagacgg
    25741 tcgctcaaat cacgccacag cgcgtgggtc aggcatttat cgccgccctg gcagccacct
    25801 ttaccctgac aacgggtggc atctacagat tcgtcaacgg cgctaatcac ctcgccaacg
    25861 gcgatgctgc tggcatcttt gcctaacaga taaccaccgc ctggtccacg tacgctggaa
    25921 accagaccat ttttacgcag acgggaaaac agttgttcca gataagaaag ggaaattccc
    25981 tgacgttcgg aaatatcagc caacggtacc gggcccgctt cagagttgag cgcaacgtca
    26041 agcattgcgg tcacggcata gcgcccttta gatgtcagtc tcatctctta cttcacctca
    26101 aactcgcccc tgcccggggt tttttattgt aaaagtgggg gtattgcata gcagggtcaa
    26161 gtctgacatt cccgagtaaa ttggtcaact atttacttga ctgatttagt cgggtattta
    26221 accttcagtg ccattttttt atcgtggcgt ttgtacctgt tgtcggatgc ggtgtgaaca
    26281 ccttatccga ccttggattc cgtgcactta caggcctgat aagacgcgtt aagcatcgca
    26341 tcaggcaacc actgttattc ggccttgtta cctttattct gctgctcaat agaagccaga
    26401 atcccgcgca ggatattcaa ttcctggctt tccgggcgtg cacgggtaaa cagacggcgc
    26461 aatttattca tcacctgccc cggatggttt tcacggataa aaccggttgc cagcagcgtt
    26521 tgctccagat gaccataaaa acgctccaga tcatcgacca gcggatacgg cgtctcttca
    26581 tgctcgacct gctcgccatt ttcctgagtt gccagccagg ccatgcgcac ttcataggca
    26641 ataacctgaa ccgccatcgc caggttcagc gaactgtatt ccgggttagc cgcaatcgcg
    26701 acatgataat ggcatttctg caactcttca ttggtcaagc cgacgcgctc gcgaccaaac
    26761 accagcgcca ccggggtatt tgccgcttca gcgacgcttt tcagaccgca ttcgcgcggg
    26821 tcgagcatcg gccacggcag cgtgcgggag cgagcgctgg tgcccaccac cagactacaa
    26881 ccggctaacg cttcgtcgag cgtatcgacg atgtgagcat taccaatcac atcgctggcc
    26941 cctgctgcca gggcaatcgc ctgggagtct ggtttcacta gtggattaac cagccacaga
    27001 ttggttaatc ccattgtttt catggcacgg gcaacagaac ccatattgcc ggtgtgtgac
    27061 gtctccacca gcacaattcg aatattttgc agcattgtct ttcttcatct aaagattatt
    27121 cacgcatctt atcataaaac gaagacagat gccgatctcg ctgctatact ctgcgccgtt
    27181 ttcccgttct ttaacatcca gtgagagaga ccgatgcatc cgatgctgaa catcgccgtg
    27241 cgcgcagcgc gcaaggcggg taatttaatt gccaaaaact atgaaacccc ggacgctgta
    27301 gaagcgagcc agaaaggcag taacgatttc gtgaccaacg tagataaagc tgccgaagcg
    27361 gtgattatcg acacgattcg taaatcttac ccacagcaca ccatcatcac cgaagaaagc
    27421 ggtgaacttg aaggtactga tcaggatgtt caatgggtta tcgatccact ggatggcact
    27481 accaacttta tcaaacgtct gccgcacttc gcggtatcta tcgccgttcg tatcaaaggc
    27541 cgcaccgaag ttgctgtggt atacgatcct atgcgtaacg aactgttcac cgccactcgc
    27601 ggtcagggcg cacagctgaa cggctaccgt ctgcgcggca gcaccgctcg cgatctcgac
    27661 ggtactattc tggcgaccgg cttcccgttc aaagcaaaac agtacgccac tacctacatc
    27721 aacatcgtcg gcaaactgtt caacgaatgt gcagacttcc gtcgtaccgg ttctgcggcg
    27781 ctggatctgg cttacgtcgc tgcgggtcgt gttgacggtt tctttgaaat cggtctgcgc
    27841 ccgtgggatt tcgcggcagg cgagctgctg gttcgtgaag caggcggcat cgtcagcgac
    27901 ttcaccggtg gtcataacta tatgctgacc ggtaacatcg ttgctggtaa cccgcgcgtt
    27961 gttaaagcca tgctggcgaa catgcgtgac gagttaagcg acgctctgaa gcgttaatga
    28021 ctcaggcggg tgatatcact cacccgccct cgcctttcag gcgctattcc gaaatacttc
    28081 ctcaccgctt tactttcttt cgtcactctc ccaccatctt tccccgatta atggatgata
    28141 aagaagtcgc aaggaaaggt tatggcgctg ccagtgaaca aacgcgttct caaaattctg
    28201 tttattctct ttgttgttgc cttctgcgtt tatttagtgc cgcgcgtcgc catcaacttc
    28261 ttctattatc ctgacgataa aatatacggt cccgacccct ggtcggcgga atccgtcgaa
    28321 tttacggcta aggacggtac tcgtctgcaa ggctggttta ttccttcttc gacgggccct
    28381 gctgacaacg ccatcgcaac catcattcat gctcacggca atgccggaaa tatgtccgcc
    28441 cactggccgc tggtcagttg gttacccgag cgtaacttca acgtttttat gtttgattat
    28501 cgcgggtttg gtaaatcaaa aggcacgccg tcccaggccg gattgctgga cgatacgcaa
    28561 agtgccatca atgtggtgcg ccatcgcagc gatgtaaacc cacaacggct ggtgctgttc
    28621 gggcagagta ttggcggggc gaatattctg gatgttattg gtcaggatga tcgtgaaggc
    28681 atacgtgcgg tgatcctcga ctccaccttt gcctcttatg cgaccatcgc caaccagatg
    28741 atccccggca gtggctactt acttgatgag agttacagcg gcgaaaatta catcgccagc
    28801 gtcagcccga tcccgctttt actcattcac ggtaaagctg atcacgttat cccgtggcag
    28861 cacagtgaaa agttgtatag cctggcaaaa gaaccaaaac ggttgatcct aatcccggat
    28921 ggcgaacaca ttgatgcttt ttccgatcgt cacggcgatg tttatcgcga acagatggtg
    28981 gattttatcc ttagcgcatt gaatccgcag aactaaccta tgatcgctag cacgataatc
    29041 attcacaaaa ccaccttaag acatgctaat ccactggtca gaacagttta agatgagaaa
    29101 aattctgtga tgcttgccaa catttctgat gattagcatt cccttcgcca tttccctgag
    29161 caaactttag ctattcttat caattatgct tatgggagat ctacagatga tgcctacgct
    29221 tgctccacca tctgtccttt cggctcccca gcgccgctgc cagatcttgc tgacactctt
    29281 tcagccgggg ttgaccgcca ccacggcaac tttcagcgag cttaatggtg tggatgatga
    29341 tattgccagt cttgatatca gcgaaacagg acgggagatc ctgcgctatc atcaactcac
    29401 actgactact ggttatgacg gcagctaccg ggttgaaggt acagtgctta accaacgttt
    29461 gtgtttattt cactggctat gacgtggttt ccgtctgtgt ccgtcattta ttaccagccg
    29521 attcaccccc gccctgaaga gtgaactgaa gcggcgcgga attgcgcgta acttttacga
    29581 cgataccaat ctacaagcgt tagtgaatct ctgctctcga cggctgcaaa aacgctttga
    29641 aacgcgcgat attcatttcc tgtgtctgta tttgcaatat tgtttgttgc aacatcacgc
    29701 tggaattacg cctcagttta atccgctcca acgtcgctgg gcagaatcct gccttgaatt
    29761 tcagatagcg caggaaattg gacgccactg gcagcgtcgg gcgctccagc ctgtaccgcc
    29821 tgatgagcca ctatttatgg cactactttt ttccatgttg cgggttcccg atccactgcg
    29881 ggatgcgcat cagcgggaca gacaactgcg tcagtctatc aaacgtctgg taaaccattt
    29941 tcgtgagctg ggaaatgttc gtttttatga tgaacagggg ttatgcgatc agctttatac
    30001 ccacctctcc caggcgttaa atcgcagttt gtttgccatc ggtattgata acaccctgcc
    30061 ggaagagttc gcccgcctgt atccacgcct ggtgcgcacc acccgcgcgg cgctggccgg
    30121 atttgaaagt gaatacggcg tccatctttc tgatgaggaa agcggtctgg tcgcggtgat
    30181 tttgggtgcc tggctaatgc aggaaaacga cctgcatgag aaacagatta ttctactgac
    30241 cgggaatgat agcgagcgag aagcgcagat tgaacagcag ctacgcgaac taacgttact
    30301 gccgctcaac attaaacata tgtcggtaaa ggcatttttg cagacaggcg ctccgcgcgg
    30361 cgcgacactg attattgcgc cttataccat gccgttaccg ctcttttcac caccgctgat
    30421 ctatacggac ctgacgttga caacacatca acaggagcag atccgcaaaa tgctcgaatc
    30481 agcatgaggg aacaactttc gggcgcaaaa acattgccgg aagcgccacc agcgccatca
    30541 cccagaacac gccgtggccg agatattgat acaggaaacc ggcgaaaacg gtcatgatgg
    30601 cgatactgcc gcccatcgcg acggcagagt aaaccgcctg taaacggatg acttcgctac
    30661 cctggcgagc agcaatatag cgcatggcag ccaggtggca gaccgtgaag gtaccgcaat
    30721 gcagaatttg caccactatc aaccacggca acgccgtagt tgctcccata atgccccagc
    30781 gcactacgcc gcaaatcgcc gagatcaaca gcatatcgcg tgcactacaa cggcggaaaa
    30841 gtttattact cagcgcaaag ataatgactt ccgccaccac gcccagcgac cacaaatacc
    30901 ccaccgccga ggccgagtag ccagctgcct gccagtaaat ggcgctaaaa ccgtaatagg
    30961 ccgcatgtgc cccctgcaat aaacaaacgc aggccagaaa gcgccagttc tggcgaacca
    31021 acgccaacca cgcagaccag ccggtgctct cctgctggcg gcttgcccct tgtggctgaa
    31081 tcgtcggacg gatgagaaag ccgagcagca tggatgccac gcccaacgtc aacagcgcga
    31141 ggatcacccg ataatcaaac atactgacca gtttgcccgt cagcgccgag ccaatgacaa
    31201 acgccaccga gccccacagt ctcactttgc cgtaatcaag cgggaactgc ttttgccacg
    31261 tattcgccag tgcatcggtc aacggtacca gcggtgagaa aaagaggtta aagccaatca
    31321 tcaccagcat cagccacgct acgtgcgccc ccgcccagaa ggcgacagca aagagaagtg
    31381 tcagcagtgc cagcacgcgc aaggcggaaa tcaggcggga aggatcgctg acgcggggcg
    31441 cgatgagcaa actcccgagg aaacgggcaa ccagacccac ccccaataac aggccgatgg
    31501 tttctggcgt taaaccaatc cctttaagcc agacgctcca gaaaggtaga aaaatgccgt
    31561 aactaaaaaa gtatgtgaaa tagccgagcg ccaaccagcg cgtggattgc aaaaccatga
    31621 gtccctcccg tcaggaggcg ttagtctgag gttaatcgaa tgttggtgca agttgaaaaa
    31681 taagaatatt cgagcctgct tcctgacggt tatcctgcat atcaggaaat gacaaaaaag
    31741 agcggcccac aacacaacat gtaaaaaaga acgatgaaac aggtgaaaat cgcaatttat
    31801 tgaactgaca aactcatgtg agacggataa ggcgtgcatg tcgcatccgg caggaaacaa
    31861 taagcacatt gtcagccatc tgcgttatgc cgttatacca gaaacgaata tctcaacgcc
    31921 tgggcgtggc gaggtaccag agtgtgtatt acgtttaaat cacatcatct tgcaaaggga
    31981 ttggttatga actcactacg ttattttgat tttggcactg cccgccccgt tttattatta
    32041 attgcccgta tcgccgtggt cttaattttc attatttttg gttttcccaa aatgatgggc
    32101 tttgacggta cggtccaata tatggcctcg ctgggcgcac cgatgccgat gctggcggca
    32161 gttattgcgg tagttatgga agtacccgcc gcgatattaa tcatacttgg ctttttcacc
    32221 cgtccgctgg cggtgctgtt tattttctac acgctgggaa ctgcggtgat tggtcatcat
    32281 tactgggata tgactggcga tgcggttggg ccaaatatga ttaatttctg gaagaatgtc
    32341 agtatcgctg gcgcattcct gctgttggca attaccgggc cgggggcgat ttctctcgat
    32401 cgccgttagg aaagatgccg gatgcggcgt aaacgcctta tccggcctac cgctccaaca
    32461 ccgattgtag gcctgataag acgcagcaag cgtcgcatca ggcatcgtgc accgagtgct
    32521 acaaaatcga aattaccccg taggcctgtg catccggcat tttataaatt acagcaacgc
    32581 aacccgcaac gacatctctg aaattgttga ttcccctggt gcaagcgcaa tgaggtcacc
    32641 accttccgga cgatgatggt catccggcgc atggctcatc ggttccagac agaagaaatc
    32701 aaacgcatat cctttatcaa acgccgggtc ggaaacaaag atgaaataac acggtgcagg
    32761 cggcgtcgtt tccatgatga tggcataacc ctcctgcggc tgctcgatgc gggcctgacc
    32821 attccatccg gcaaaaccat tgtttaccca ctggcgcggt aacggcgcga gctggttaaa
    32881 atccagttcc tgcggtagct gctcgcaaaa ctcacccgcc agtaaccgct cgcctgcgcc
    32941 tgaatccgcg tttgtggtga caacggaaaa taaggatgcc agccggtacc aaatggcagc
    33001 gtctctgccc ctttattggt gacagcgagc gtcaccgtca gcgtatccgc cgttaaatga
    33061 aacgcctgac ttacccgata gtgataaaca ccgctgcgat gttcatacac cagacacaag
    33121 ctatcttcgc tgcgcgagac acattgccac tgccccagcc aggcatcacc gtgtagatag
    33181 tgcgcatccc actcaacatt cggttgtagt tgatattcac gtccctgcca gacaaaacga
    33241 ttaccgctca cccgattagc aaacggaacc agcggaaaac aggaagcgtc ggtagcaacg
    33301 ccgctttttt tccccggacg cagcagcggg gcggcgtcac gccagaagcc ttcaattaca
    33361 ccgccctgat cggaaacctc aagctttagc gccccctgag atagggtata gatggtcatc
    33421 ctctactcct taatccggga agttgatcac gactttcccg catttgccgc tcgccattag
    33481 cgcgtaagca tctcccgcct gctccagtga gaagcgatgg gtaatagcgt tacgcggcca
    33541 cagtttccag tcagtcaaat cgtgggcgca tttttccata tggaacaagc tggtcaccca
    33601 ggagccaata attctgcgtt ggtgatgcat caggtcagcg ctgacctcaa attccacttt
    33661 tccggtttcg ccaatgtaaa ccactcgccc ccagtcggcg gtagattgca atgccagcaa
    33721 gcgccccgca gcattaccgg aacaatcaag cgcaacatcc gcaccgccat gagtgagttc
    33781 agcgatgatc tgcggcagac cttcggtggt cgctaaatag ccgtgatcca tcacccctag
    33841 ctgtttcgcc atcgccagac gttccggcaa catatcaacg ccgatgatcc tttttgcacc
    33901 acgacctttc gccagcatca tcgccatcat gccgaccggc cccagaccga ccaccagtac
    33961 gttatcactg ccggaaactt cgccgcgcaa aatcccttca tacgctgtac caacgccgca
    34021 actaataaac gcaccatctt cgtaactcag tgcatccggc aggaggatca gatctttttc
    34081 ttccgccagc aggtattcag catggccgcc gtcacgctgc cagccgtaag ccgcttttcc
    34141 ttcgccagta caagagatag gaaaaccacg acggcagttc gggcaaaaac cacagccaga
    34201 aatatgatac accagcacac ggtcgccctc tttaaaatgg cggcagcctt gccccatcgc
    34261 cacaatctgc ccgcacggtt catgaccgtt gataaatccc tggtataacg gtttatcggg
    34321 tgccgccgcc gtagcacggt gctggtggta gatatagtgg acatcgcttc cgcaaatccc
    34381 ggaggatttc attttgatca gtacctggtt aatccccggc gtcggcaccg caacttcccg
    34441 cagatcgacg gtcgaatttc ctggtaaata agctgccagc atcgttttca taaatccctc
    34501 attaacaata cgattaattt tcatccctgc cgcacccgcg ccaggggcgt taaggtttag
    34561 cgtttcgctt tactgctacg ctgggtcagc aagatattcg ccagcaccgc caccacgatg
    34621 atgacgccgc gcaccacctg ctggaaaaag gagttaatcc cgagcagcac cagaccgtta
    34681 ccgattagcg taatcaccag tacgccaagc agcgtaccga acaacgagcc gcgaccgccg
    34741 gaaagcgccg taccgccgac cacgaccgcg gcgatgacgt caaactccag accgtttgcg
    34801 gcacctgcgt taccggaacc gaggcgggcc gccaacaaaa tgccggtcac cgccgctaat
    34861 aatcccgaaa gggtaaaaat aagaatgcgt acccgacgaa cgttaatacc gcacaactgc
    34921 gctgcagtgg cattaccgcc aacagcaaaa accgagcgcc cgaaggcagt tttgcggcta
    34981 atgaacacaa acagcgcaaa taacaccatc atgatcagcg cggataccgg cacaccgaga
    35041 aattgtccgc ccagccagtc cagcacttcg ttttcgttaa ttggcactgg caaggcgttc
    35101 gtcataaaca gccccattcc gcgcagggcg ctccataaac ccagcgtggc aacaaaactt
    35161 ggcacgttaa acacgccgcg cagcaccccg gcgagcgttc ccatcagcgc acctaacagc
    35221 aacaccagca gacaagcaat cgccagcgga acgtcaaatt gcagcaaaaa cgccaggcac
    35281 actgaaacaa aagccaccat cggcccaacg ctgacatcaa tttcaccgga gataataatc
    35341 agcgtcatcg cccaggcggc gatcccgatg gtggcggcat cgcgcagcac gttcatttgg
    35401 ttattcaatg agataaagcc aggcgcattc agggagaaga ccagataaag aatggcaatc
    35461 accaccagca aaccgatctc attaatatgg cgactgacaa attgtttgag cgagacgctc
    35521 ttgccctgcg gcaatggtaa tgacgaagca gacatcttca ggttcctcgg taaaattcat
    35581 cagtgcacag acagaatggc ggacatcagc tcatccacat tgaccggaga gtgaaactcc
    35641 tgcgagaacg taccgtgctg taacaacaga atgcggtcgc acaccaacgg taactcctcc
    35701 acttcactgg agataaacac cacgcttttt ccttcggcag ccagctcgcg gacaatacgg
    35761 taaatctgct gtttggcttc gatatcgacg ccgcgcgttg gctcgtcgag caacaaaatc
    35821 tggctggcgg catagaccca acgaccgatc accacttttt gctgattgcc gccagaaagc
    35881 gtgccgatgg gcgtttcgct actggcggcc ttgaccgtca tccgctgcat cacctcttcg
    35941 gtcaggcggc ggatggtgga ccattgcagc acaccgttgg cgctgatttt ttgccgattg
    36001 gtcagcactg tattttcgtc aacgcccaac cagggaatga tccccgcttc tttgcggttt
    36061 tctggcgtat agccaatgcc gcgtttcagc atgtcgccgt aatcggggcg cgtgattttc
    36121 tcgccgttga taacaatttc gccctgttca tacgtctcca gaccaacaat cgccttcagc
    36181 aattcactgc gccctgcccc cagcagacca gcaatgccga gcacttcgcc acgacgtagc
    36241 gtaaaactga tatcctccag cttgggctta tggcgtaacg cacggacttc cagcacggcc
    36301 tgatccataa tttcctgagg ggcaaccggc gcaatatcaa cgtgatcgcg cccgagcatt
    36361 agcgacacaa tatgatgcgt agaggtgttt tcgagcatca catcgcccgc cacctgacca
    36421 tcgcgcataa cggtggcaca ggaggcaatg cggcgaattt cttccatccg gtggctgaca
    36481 taaatcaccg ccacgcccag tgccgacatc tttttcaccg cgctgatcac cagttcaact
    36541 tccgcactgg caagcgagct ggtgggttca tcaagaatga ccacgcgcgg ctcgcccttc
    36601 atcacccgcg caatttccac cagttgcttt tgcgccgggc ttagtgttga aacaagttgt
    36661 tcaggactaa cgtcaacgcc caacgcctgc aagcaacgtt gggcgtcctg cgccatttgc
    36721 aggtaatcaa tcatgccgtt gcggcggggc cactgaccga ggcagaggtt ttccgccact
    36781 gtcagccctt ccaccagact taactcctga taaaccgcac gaacccccag ttcagcggca
    36841 cggcgagtca gcgtagcttc gtcaccttcc agtcgcgtct cgccaatcca gatatcaccg
    36901 ctatccggac gttcgctacc ggtcagcatt cgaatgaggg tcgattttcc cgcaccgttt
    36961 ttgcctaaca gcgcgcgaac ttcgccttta ttgagcgtga agttaacgtt atccagcgca
    37021 acgacgccgg ggtaacgctt atttcctgcc accacttttg ctaccgggac tgcctctgtt
    37081 gccgtgaaca tagggacctc tgcgaatcag cgattagggc agaccatcaa cgtgcgttgc
    37141 cagccactgt ttaccgtctt ccgttttggt atagagatcg attggcacct gaatcacttt
    37201 ttcaccgtcg gcttgtttat tgataacctt caatgtttgc gtaaaaacag cattgcccat
    37261 tttcttaccg gaaatatcca ctaccgcttt cagcacctgg ttattttcca gttcctgagc
    37321 aatttcagtg gtcatatcag aaccgaaaac agcaattttt ccggcctgat tttgattacg
    37381 taccgctttt accgcgccga gtgtcgcacc gcccgattcc cccataatgg cgttgagatc
    37441 cggcgtggag ataatcagtt tttcaccgac agaaatcgct ttatctaaaa cagtcccttc
    37501 ctgattagcg acaatttgcg cgccgggaac gtgggttttt aatacttctt caaacccttt
    37561 acgtcgttgc acacaaactt caaaggcttc gcaattgatg acggcaattt tcggctggtc
    37621 aattttgttg gcaataaaat aatcggcggc tgcgttaccc agttttttac caaattccag
    37681 cgggtcacca accagatatg ccgagacata tttatcgaca cccttttgat taatacaggt
    37741 gttgtagcaa atcaccggaa tacccgcttc actggcgcga cgaacggtac ggctactgcc
    37801 attttcagac actgccgata aaataatggc atcgacatta cgcgccacga gggtatcaac
    37861 aaaggtactt tctttcgaaa tatcgccctg agcgttagtt tcaattaatt gtacctgtac
    37921 tgaagaatct tttgccgcat cctgaacgcc ctgacgcact ccggcgtaat atccctgggt
    37981 atcaaggtat attgcgccaa tagtcatttc tttatcagaa gccctggcaa atagtgcgct
    38041 acccaatagc gtagccatta ataataaatt acgggttctt gtcatttttt taggcatagt
    38101 gcctcctgta gggtttttat ttacaacggc ttattttaat tatttgagcg tgagcggcag
    38161 cgcgtgccgc tcaaatatta cagttaatag ggtttagtga acgtagctaa aaataaaagg
    38221 catcacggtg gtgaataatg ccgctttcgg ccaggctgga ttaatcgcca tcaattcagc
    38281 gcgagcagat tcatagtgcg caacctcccc taatcccgcg gccgccagca tcgccaccat
    38341 caggcatttt tctttatgct gctgttgtaa atcgctatac agcgacaaca agtcaggctg
    38401 cgagacggca aagaaatccg cttcgatact ggttttcgcc atctctttcg cccactgttt
    38461 catttcacta aacagttgct gtgcggtgtg ttgttcgccc agcagtcgca gcgccattcc
    38521 ttgccagaag agataatcaa ccggctgatc gttgtaataa ctgtggatgt taatggtgcg
    38581 atcgccggtc gccgccagac gtaaacaacg catcgcttca gtttcatcgc cctgcgcgtt
    38641 ggcgcatact gcctgccaga accagatgtc gttgtcagtt tgccccggta aacggccttc
    38701 gcttaaattc tccggataat gcagcgcggc atgaagcagt tcgctggcct gctgcggctc
    38761 tctggcatca agatgctgcc aggcgcgtaa taactggttg aggataaact ggctggtgac
    38821 cttcccttcc ccaccttccc acgggtggaa tttgcgcgtg gcgagaatgt ccgccgcttt
    38881 gtctgcctga ccagtgagat gccacaaatt gagcagttct gcggtcatgt cgtcgcgttt
    38941 cagcgcaatt tccagattat ttcccagacg cgccagtcgt ttctccggtg tgacaccgct
    39001 taacttatcc agcaaatccc gttcgaaaag cagacgtgca tcctgcggcg caagctgata
    39061 agcattatca agataacgcg cggccagctc ataatcgtgc tgcttattcc acgcatggat
    39121 cgccaacccg cgccagccgt cggcaaactc cggcgacatc tctacgcaac gctgccataa
    39181 ggcaatggct ttaccgtagc tacgcttgtt gtagtagaag caggccagta aatgccgggc
    39241 aaaccagcac tcttcgatac tctccagcgc cgccacttct tccagcgtat tcgggaaacg
    39301 gacaaactgc gggaaggcat caatagcttt tacgaccagt tctccgcgtt cagcttttgg
    39361 tagcaaactg gcttgcaggt aaagcggtag cgtgcgctga cagtccagtg cgttcagcat
    39421 atctgccgcc agggcgggca tgccccagtt aatcagttgc acggcggtca tcagggcgtt
    39481 aacgtcgcgt ccctgacaaa gacctcgcca ctgtacgagg gcagattcgc tacgaccatc
    39541 gaaccagttc agtcaccaca gagtagcgtt cagcggataa tcacgcagca gtttctcgcg
    39601 ctgcaaacgc gcgttgtcct gacgaccact taacaccagt agcaggttat gcaggcaaag
    39661 cacttcctga ttggttgggc tggcgcgaag actttgttgg caaaaatcca gaccagcgtc
    39721 aaagttacca ttacgcgccg ccagccgtgc cagaccataa tagccgcctg ctttactgtt
    39781 gccgctccag accgcacgcc agaaatcctc ttcggcttgt tgatattgcc cctgacgttc
    39841 gtaagcactg gcgcgaatca aactcgcctg tccgcactgc ggatttttgt tcagcgcatg
    39901 tgcgcgtttc agcgcctgag tggcatacgc caccgcttgc gggaaatcgg cacggttata
    39961 ttccagcatc gccagcgcca ggttacagcg ataatccagc ggatccagcg ccacgccgcg
    40021 caggtagtaa tcgaacggtg aacggctcgc gtgatgatat tgctccagat gttgaccgat
    40081 aaaccaggct tcatctgtac tggtaatgtc ttgtgctgcc agtggcgctt tggcgacgtc
    40141 cggcaacggc aactcttgcg gctgattttc atgataactc agtacaatat tgccgtcggc
    40201 atcggagagc tcaatggtca gcctttcagg attgataccg tgcaacacgc cctggatggc
    40261 ggtcgctggt gtcagggcca cggcatcatc gagcaacgcg ttgcatttgc cgatttcgcg
    40321 gatcgccagg cggtatccgt tcaacggaga gatggcatac agcccccact caatcccccg
    40381 ctcactacgc tggagtttta tcaccgcatc gcgggaggca ttttgcacca tgcccagaga
    40441 atgataaggc aggaaatact gctcgaaacg cttctcctcg taagcatcaa gccaggtaaa
    40501 atcaggctgg ttatcggcaa aaaaaccggt catcagttcg atatacgggc cgttattgtc
    40561 agtcaggctc ttatcccacg cctggccaaa ttcactgtgt ccccagctcc actgtttttt
    40621 acctggcgca atatggtggt tggcaacgtg tagcaaacca ccatcttcat cgtgacacca
    40681 cgcgccaaca aaatcgtact gtgatttttc agccatatat gaggttggaa cgggcacatt
    40741 tttatagcga gaaatgtcca ctccggcgga gtagtccact ttgtagtaag tgccggtggc
    40801 gatggggaaa gcggagacgg cccgtttacc gtgatcaaac accgcagtca catccggtgg
    40861 gaaaacgctc tgatgccctt cccccccttt cactgccggg ttggcccacc acaagaaatg
    40921 acgcggcgtg gcgttgccgt tatagacgcg actggcgatt tccagcgccg cccggtcagg
    40981 gcgcagggtg aaacctgtca tcacctgtaa accgtgcatc ggctccgttt cgccgaccca
    41041 caccgtctgt gcgccgtcgt catgggcttc gagggtgaaa tcaacgggca taaaggtggt
    41101 cgggcgatgg tgttgcggcc agttaaactc aatcccgccg gagatccacg gccccagcag
    41161 tcccaccagc gccggtttaa tgacttcatt gtgataaaca aaatcgcgct gtttcacttt
    41221 atcccacgcg cgatgcaccc gaccgcccag ttccggcagg atcatcactt tgatgtagtc
    41281 gttttccagc cacaccgcct gccaggattt cagggttttc tgctcgctca gcgtatcggt
    41341 cacgccgtag ggataaaccg cgccggacga tccctgataa acgcgatttt ccaggaacat
    41401 gggatgtata tcctgcggcc cggtttcata ggtcgggatc tcaacgcgct cttgccacac
    41461 ttttactgga gtcatggtgc cctcaatatt aacaagacat actgaattaa aagatttgtg
    41521 gcagtgtatt gaacaatctg gcactgtttt cgcggaataa tcacgcaatt aactaaacag
    41581 ggtttagtga agatgagagc ctgcattaac aatcaacaga ttcgccacca taacaaatgc
    41641 gtgattctgg aactgctgta ccggcaaaag cgcgccaata aatcaacgct ggcccggctg
    41701 gcgcaaattt cgattccggc ggtcagtaat attttgcagg aactggaaag cgaaaaacgg
    41761 gtggtgaata tcgacgatga aagtcagacg cgcggacata gtagcggtac atggctgatt
    41821 gcgccggaag gtgactggac gctgtgtctg aacgtgacgc ccaccagcat tgagtgtcag
    41881 gtcgctaatg cctgtttaag tccgaaaggc gaatttgaac gctttcagat tgacgctcca
    41941 acgccgcagg cgctgctgtc cgaaatcgaa aaatgctggc atcgccaccg taaattgtgg
    42001 ccggaccgca ccatcaatct ggctctggca atccacggtc aggtggatcc ggtgaccggc
    42061 gtgtcgcaaa ccatgccgca agcgccgtgg gcaacaccga ttgaggtgaa gtacctgctg
    42121 gaagagaagc tgggcattcg ggtaatggtc gataatgact gcgtgatgct ggcgctggcg
    42181 gagaaatggc aaaataattc gcaggaacgg gatttctgcg taatcaacgt tgattacggc
    42241 attggctcgt cgttcgtgat taacgagcaa atttatcgcg gcagtttgta tggcagtgga
    42301 cagattggtc acaccatcgt taatccggat ggcgtcgtct gcgactgcgg gcgttatggc
    42361 tgcctggaaa ccgtcgcctc gttaagcgca ttaaaaaaac aggcgcgggt atggctaaaa
    42421 tcacaaccgg ttaatactca gcttgatcct gaaaaactga ctaccgcgca gttaatcgct
    42481 gcctggcaga gtggagaacc gtggatcacc agttgggttg atcgctctgc caacgccatt
    42541 ggtttgagtc tgtataactt cctcaacatc ctcaatatta atcagatttg gttgtacggt
    42601 cgcagttgtg cctttggtga gaactggctt aataccatta ttcgccagac gggatttaac
    42661 ccgttcgacc gcgacgaagg accgagcgtg aaagcgacgc aaattggctt tgggcaatta
    42721 agccgcgcac agcaggtgct gggaattggc tatttgtatg ttgaggcgca gttacggcag
    42781 atttgatggc gcaataacgt agaaaggctt cccgaaggaa gccttttttc aacagattaa
    42841 ccgtttcgct tatgcgtaaa ccggataacg tgcgcagatg tcgagaactt tacctttgat
    42901 gcgctcgata acagcttcat cattgatgct gtccagcacg tcacacatcc agccagccag
    42961 ttctttcgct tcggcttctt taaagccacg acgggtaatc gccggagtac ctacacgaat
    43021 accggaggtc acaaacgggc tcttcggatc gttcggtacg ctgtttttgt tgacggtgat
    43081 gttagcacgg cccaaggcgg cgtcagcttc tttaccggtc aggtttttat caaccagatc
    43141 aaccaggaac aggtggttat cagtgccgcc ggaaaccact ttgtagccgc gttccaggaa
    43201 cacttctacc atcgctttag cgtttttcgc aacctgctgt tggtaagttt tgaactcagg
    43261 ctccatcgct tctttcagag caaccgcttt accagcgatt acgtgcatca acgggccgcc
    43321 ctgaccacca gggaaaacgg cagagttcag ttttttgtac agctcttcgc taccaccttt
    43381 cgccaggatc aggccgccgc gcggacccgc cagggttttg tgagtggtgg tagtaacaac
    43441 gtgagcatga ggaaccgggt tagggtagac accagcggca accaggcccg caacgtgcgc
    43501 catatcaacg aacaggtaag caccgatgct gtcagcgatt tcacgcattt tcgcccagtc
    43561 aaccacgccg gaatatgcag agaagccacc gataatcatt ttcggtttgt gttctttggc
    43621 ttgtttttcc agatcggcgt agtcgatatg accggtagca tcgataccgt aaggaacgat
    43681 gttgtacagt ttaccggaga agttaaccgg agaaccgtga gtcaggtgac cgccatgcgc
    43741 cagattcata cccagaacgg tatcgcctgg ttccagcaac gcggtgtaca ccgcaaagtt
    43801 agcctgggag ccggagtgcg gctggacgtt agcgtagtca gcgccgaaca gttctttcgc
    43861 acgatcgatc gccagttgtt caacgatatc aacatactcg caaccgccgt agtagcgttt
    43921 gcccggataa ccttcagcat atttgttggt cagctgagaa ccctgcgcct gcattacgcg
    43981 cgggctggtg tagttttcgg aggcgatcag ttcgatgtgc tcttcctgac gtactttttc
    44041 ctgctccata gcctgccaca gttcggcatc ataatcggca atgttcattt cacgctttaa
    44101 cattcgcatc tcctgactca gctaacaata aaatttttgg cctttatagg cggtcctgtt
    44161 ggacaacggc gaacagtata accgaatcat tgtgcgataa caggtcttga caaaggaatt
    44221 tacgcaaacg attaccttca ggctacgcaa ggctttggag aataaagagc ttgtaaccgg
    44281 aaacggattt cttttcaggt ttgtgatgca aatttttcac ttcatcacat tctttctgaa
    44341 aaacaccaaa gaaccattta cattgcaggg ctatttttta taagatgcat ttgagataca
    44401 tcaattaaga tgcaaaaaaa aggaagacca tatgcttgac gctcaaacca tcgctacagt
    44461 aaaagccacc atccctttac tggtggaaac gggaccaaag ttaaccgccc atttctacga
    44521 ccgtatgttt actcataacc cagaactcaa agaaattttt aacatgagta accagcgtaa
    44581 tggcgatcaa cgtgaagccc tgtttaacgc tattgccgcc tacgccagca atattgaaaa
    44641 cctgcctgcg ctgctgccag cggtagaaaa aatcgcgcag aagcacacca gtttccagat
    44701 caaaccggaa cagtacaaca tcgtcggtga acacctgttg gcaacgctgg acgaaatgtt
    44761 cagcccgggg caggaagtgc tggacgcgtg gggtaaagcc tatggcgtcc tggctaatgt
    44821 atttatcaat cgcgaggcgg aaatctataa cgaaaacgcc agcaaagccg gtggttggga
    44881 aggtactcgc gatttccgca ttgtggctaa aacaccacgc agcgcactta tcaccagctt
    44941 cgaactggag ccggtcgacg gtggcgcagt ggcagaatac cgtccggggc aatatctcgg
    45001 cgtctggctg aagccggaag gtttcccgca tcaggaaatt cgtcagtact ctttgactcg
    45061 caaaccggat ggcaaaggct atcgtattgc ggtgaaacgc gaagagggtg ggcaggtatc
    45121 caactggctg cacaatcacg ccaatgttgg cgatgtcgtg aaactggtcg ctccggcagg
    45181 tgatttcttt atggctgtcg cagatgacac accagtgacg ttaatctctg ccggtgtcgg
    45241 tcaaacgcca atgctggcaa tgctcgacac gctggcaaaa gcaggccaca cagcacaagt
    45301 gaactggttc catgcggcag aaaatggcga tgttcacgcc tttgccgatg aagttaagga
    45361 actgggtcaa tcactgccac gctttaccgc acacacctgg tatcgtcagc cgaatgaagc
    45421 cgatcgcgct aaaggtcagt ttgatagcga aggtctgatg gatttgagca aactggaagg
    45481 tgcgttcagc gatccgacaa tgcagttcta tctctgcggc ccggttggct tcatgcagtt
    45541 tgccgcgaaa cagttagtgg atctgggcgt gaaacaggaa aacattcatt acgaatgctt
    45601 tggcccgcat aaggtgctgt gatttgatgt tgccggatgg aaacatccgg caacccttga
    45661 cccgttttaa atcgccgcat catcctcttc accggtacgg atacgaatga cccgtgccac
    45721 gtcgaagacg aagattttac cgtcaccgat tttgccggtt tgcgccgtgc gaataatggt
    45781 atcgacacag gtatcaacaa tgtcgtccgg tacgacaatc tcaattttca ctttcggcag
    45841 aaaatccacc atatactccg caccgcggta cagctcggta tggcctttct ggcggccaaa
    45901 acctttcacc tcagtgaccg tcatgccggt aataccgact tcagccagtg cttcgcggac
    45961 atcgtccagc ttgaagggtt taataatcgc atcaatcttt ttcatgctat tccttgaaaa
    46021 ggtcgcctgt cttttgatct gctaaacgta acacataacg ccaattcatt ccttgaaatc
    46081 gtttgcatcc agttcgtgtc gggaaagcag tttataaaat tctgtccggt tgcgccccgc
    46141 cattctcgcc gcgtgggtga cgttgccttt ggtgatttgc agcagcttac gcaaatagtt
    46201 gagttcaaac tgattacgcg cctcaacaaa ggttggcagc gccgtgtttt cgccctccag
    46261 cgcctgctcc accagcgcat cactaattac cggagatgat gtcagcgcca cgcactgttc
    46321 aatcacgttg accaactggc gcacgttgcc cggccagctc gcagtcatca ggcgtttcat
    46381 tgcatcggtg gagaacgcgc ggacaaacgg tttatgtcgc tctgccgcct ggcgcaacag
    46441 gtgatttgcc agtagcggaa tgtcttctgt gcgctccgcc agggccggaa ttttcaggct
    46501 gacaacgttg aggcggtaat agaggtcttc acggaattcc ccgcgcgtca tcgcttttgg
    46561 caggtcgcgg tgggtcgcag aaataatccg cacgtcgata tcaatatcgc ggttactgcc
    46621 cagcgggcgc actttacgct cctgcaacac gcgcagcagt ttgacctgca acggtgcggg
    46681 catatcgcca atctcatcga gaaatagcgt accgccttcc gccgcctgga ataaaccttc
    46741 gcgattgctg acagcgccag taaacgcgcc acgtgcatga ccaaacagct ccgactccag
    46801 caattgctcg ggtaatgcgc cacagttaat agcaataaat ggcttgctgt tgcgcgggct
    46861 ggcgttgtgg atagcctggg cgaaaatctc tttcccggtg ccgctctggc cgttaatcaa
    46921 aacgctaacg tctgattgtg ccaccagccg cgcctgttcc agcaagcgca gcatcagcgg
    46981 gctgcgggtg acaatcgcct cgcgccaacg ttcgtcggtg gctggcgcgg attgttccag
    47041 cgcatcgtca attgcctgat atagcgcatc tttatcgaca ggcttggtga gaaaactgaa
    47101 cacgccctgc tgtgttgcag caacggcatc aggaatagaa ccatgcgcgg taagaataat
    47161 tactggcatt cccggctgca ctttctgaat ttcagcaaat agctgcatac catccatttc
    47221 atccatccgc aggtcgctga tgactaaatc tactttttcg cgattcagta cccgtaatcc
    47281 ttcaacgcca ctttccgccg tgaccacgct gtagccttcg ctggtcaggc gcaggccaag
    47341 cagtttcagc aatcccggat catcatcgac cagtaataaa tgcgcaggtt tatggctcat
    47401 caggagtgac ctcatcggtg gatggcgcgg gcttttcact ctcgtgcggc gtatccggcg
    47461 agaaatttcc agccggtttg cgggtcgaga gctggcgttc aatatcggtc aggttttcca
    47521 gcttgcgggt agtgagttcc agttgctgtt gtagaacgtg atgttgctgg cgcaaagtat
    47581 ccagctcgct gtcgctggac tgctggagtt tgctgtagcg ttggcgctct tccgccagtt
    47641 gcagttgcag cgcctggccg tcgcgccaga gttgatacag tgggcgaacc tgtgccggga
    47701 tctcggtact tagcgcatca atgcgcgcga ccagctggcg gcgctcatac ggcgtaattt
    47761 tggcgtcggc gagcaaaatc ccttgtttaa aggtattttg ccagttgccg tcgtcatatt
    47821 ggcgggcttg ctgacgcgac tgcgcaggca ttaaacgatc agcacaatcc atcgcccgca
    47881 gccagtaaag cggattggtt tcggttgatt taccttgcag cgcccagatg tcgctacatt
    47941 cagtagaaag atagtcagcc agttgataaa ccggaatttt ttcttctgct ggcgtatcaa
    48001 tggctggctt attgtgactc tgcacgcaac ccagcaatgc cagacatggc agccctgcca
    48061 gccacagccg tcggggcaat aatcgttgaa aaatgtgtcg catattcacc agacttaaag
    48121 cctatcccag tgggcgtaat tgttgcaggc agtttggaca tggacagcgc ggagaaacca
    48181 gagcgtacat gtcgtacgtg aagatttcga gcactgcccg gggccgaact gacaaataaa
    48241 atagcctgat gggatgggct gttagcatta tttcgtgttt ttcgacgacg gtaattcaat
    48301 gcggaaacaa acgtcttgcc cgctctcgtc gaccagatac agttcccctt gcatacggcg
    48361 aatacaatcc ctggcaatgc ttaatcccag accgctgccc ttcaccgccc cttttcgctg
    48421 gtggcttccc tgaaaaaagg gttcgaagat catggcgcgt tcctcttgcg gaatgggcgt
    48481 gcctgtattg atgacatcaa tataaacccg cgcaccatgt aaactgctgc gaaggcaaat
    48541 gttaccggat tcagccccgt agtgcaccgc attggagtaa agattatcca gtacgctcat
    48601 cagcagcatt ggcttcgcca ggcaagctgt tgctttgaga tcaacgtcgg tatgcatcat
    48661 tttagcccgt gcgggcaggc tatgagcaga aaccactgtc tccaccagcg gtgctaactc
    48721 aacattctcc agttccaccg cactgtccgc ctgtttacgg ttgtaatcaa gcagttgttc
    48781 gatcagcttt tgcaaattgc ggctgctgct atcaagaatg ctcaccacct ctttttgctc
    48841 tggcgtaagc ggcccgacaa cctgatcagc cagtaattca gtgccctcgc gcatactcgc
    48901 cagtggcgtt tttaattcat gagataaatg tcttaaaaat tgatggcgtt gggattccag
    48961 ccatgacagg cgctcactta accaaagaat acgttgccca accgagcgta actcgctcgg
    49021 tccactgaac gagacgctat tgcccagaga acgcccttcc cccagccggt tgatcatgcg
    49081 ctcgatattt ttcaccggac cgataatcat cctcgtgaaa agcagtacca tcaccagact
    49141 caccagaaat agcaccagcg attgccaacc aaaatattgc ccacgttcgg cgatttcacg
    49201 ctgaagttgc tgcccacgag agaacaccac tgtgcgcgtg gcctgcacca tttcggtatt
    49261 ggcactggca aaggcttcca gacgcgcggc ggcagcagca tcgggaccgc tgttgttaca
    49321 ctgaagttga gccagattgt tcaaatcctg acgtaatgcc tggtagagtt tatcgtccgg
    49381 cagcacgcct gcgtgggcat cgagcatttc gctgtaacgc ttgcgctggc tttgataaac
    49441 cttcgcaagc gtagggtcgt ccagcacgca gtactgacga tagctgcgct ccatctccag
    49501 tgccgcgttg ctcatcgctt cactgcgccg ggcatcaata agcgtagtgc ggttaaccag
    49561 cgccgcctga tcgctaagtg cattcaggct ttgccaggct tgccatgcca gcaccaacag
    49621 gggcagcaga atcagcaaaa atgccagcat taccagttgt cgtaatgagc ggggaaaaac
    49681 gggccagcgt ttcaaggcgt tactctcgtc agacgcgaat agcctgatgc taaccgaggg
    49741 gaagttcaga tacaacaaag ccgggaatta cccggctttg ttacggaata aggcggtgcc
    49801 taactcgacg tttcgcccga tggttgatat agctacgctg atatcagaag ttggacggca
    49861 ggcaccttgt tgtgcgtcat tcgaatttta tgtagcacgt cccgaagggg ctgacataag
    49921 tcggcgaatg agccactgct tactattatg cagtaactgt gccaataaag aaaatagttt
    49981 ggtaacatac tgattatacg ttgctttgag gggtttatgg cttctccgct atttgaatgt
    50041 aaatcagcca ctgtgtcgct aaaaagagac aacttaagat aaacttatta gataatatta
    50101 aaatcaatga gttaagtgtc accaaaaagc gacacggcaa accacccatt gtcgtgattt
    50161 acagacacaa aaaagctccc gaagttggga gctcataata gcggttggtg cttaatgccg
    50221 gatgcggcgt gaatgcctca tccgggctgc aataccaatg ggttgacgac ttaccccaac
    50281 tgcttacgtg cattgcggaa aatacgtatc catgggccat cctcgcccca gttttccgga
    50341 tgccaggagt tgctgacagt acggaaaact cgttccgggt gcggcatcat aatggtgact
    50401 cgaccacttt cagtcgtgac cgccgtaata ccgttcggtg aaccgttcgg gttagccggg
    50461 taggtttcag tgactttgcc gaagttatcg acatagcgca gtgccaccag ccctttgctt
    50521 tccagtgccg ccagatgcgc cgcatcgcgc acttcaacgc gcccttcacc gtgagagaca
    50581 gcaatcggca tctgcgagcc caccatcccc tgcaacagca gcgacgggct ttgggttact
    50641 tctaccagac tgaaacgcgc ttcaaagcga tcggaggtgt tacgcacaaa acgtggccac
    50701 aactcgctac ctgggatcag ttcacgcaga ttagacatca tctggcaacc gttacatacc
    50761 cccagcgcca gcgtttgcgg acgatggaag aaggttgcaa actcatcgcg tacacggtca
    50821 ttgaacagga tagacttcgc ccaaccttca ccagcgccca gcacatcacc gtaggaaaaa
    50881 ccaccgcacg ctaccaggac gtggaaaccc tccaggcccg tgcgtccagc cagcagatcg
    50941 ctcatatgca cgtcgatagc atcgaagccc gcacggtgga aagctgccgc catttcaaca
    51001 tgggagttca caccctgctc acgcagtacg gcaactttcg ggcgcgcgcc agtggcaata
    51061 aacggtgctg ccacatcttc gttgatatca aacgacagtt tcacattcag gcccggatcg
    51121 gcgtcgtttg atttcgcctg gtgttcctga tcggcacact ccgggttgtc acgcagacgc
    51181 tgcatctgcc aggtagtttc tgcccaccag acacgcaacg tggtgcggct ttcgctaaat
    51241 acagtctgcc cgttggcggt aatcacaaaa cggtcaccgg aaaccgcctg cccaacataa
    51301 tggacgcaat cagcaagccc atgctgtgcc agtaccgctt caacggcttc acggtcagcg
    51361 gctcgaacct gaatcaccgc acccagttct tcgctaaaca atgcagccag gcgatcgtca
    51421 cccagagtgg cgatatccgc gtcaatgcca caatgaccag caaaggccat ttccgccagc
    51481 gttaccagca ggccgccatc agagcggtcg tgatacgcca gcagcttacg ctgtgcaacc
    51541 agcgcctgaa tcgcgtcata gaagcctttc agttgggcaa catcgcgtac atctgccggt
    51601 ttgtcgccaa gctgacgata aacctgcgcc agtgccgttg cgcccagcgc gttattgcct
    51661 ttgcccaaat caatcagcaa cagtgcgtta tcttcggtag aaagctgcgg cgtaatggtg
    51721 tgacgtacat cttccacgcg ggcaaatgcg gaaatcacca gcgacaacgg cgacgtcatt
    51781 tcgcgctctt cgttaccttc ctgccagcgg gttttcatcg acatagagtc tttacccacc
    51841 ggtatcgtca ggcccagcgc cggacaaagt tcttcgccca cagctttaac ggcttcatac
    51901 aggcccgcat cttcaccagg gtggcctgcc gccgccatcc agttggcgga aagtttaatg
    51961 cgtttgatat cgccaatttg tgttgcggcg atgttggtta gcgcttcacc gactgccaga
    52021 cgggcagaag cggcgaaatc cagcagcgca actggcgcac gttcgcctat tgccatcgct
    52081 tcaccgtagt agctgtcgag gctggcggta gtgactgcac agttagcgac aggcacctgc
    52141 cacggaccca ccatctgatc gcgcgccacc atgccggtta cgctgcgatc gccgatggtc
    52201 accagaaagg ttttctccgc cacagtcggc agatgtaaca cacgtctcac cgcgtcagcg
    52261 atggtgatcc cttcacgggc cagcgcgtcg cctttcgctt tcagcgtttg cacatcgcgg
    52321 gtcatcttcg gcgttttgcc aagcagtacg tcgagcggca gatcgatggg ttgattatca
    52381 aaatgacgat cgtgcagaga aagatgtaat tcttcggtgg cctcaccaat caccgcgtac
    52441 ggtgcgcgct cacgcttaca cagttcgtca aacagcggta actgatcggc agcaactgcc
    52501 agaacgtagc gttcctggga ttcgttacac cagatttcca gcgggctcat gcccggttcg
    52561 tcgctcagga tatcgcgcag ttcaaattta ccgccgcgtc cgccgtcgct caccagctcc
    52621 ggcatggcgt tagaaagacc gcctgcgccg acgtcgtgga taaacagaat cgggttcgcg
    52681 tcgacaagct gccagcagcg gtcgatcact tcctgacagc gacgttccat ttccgggtta
    52741 tcgcgctgaa cggacgcgaa atcgagatcc gcgtcagact ggccggaggc catggaagag
    52801 gccgcgccgc cgccaagacc gatgttcatt gcggggccgc cgagaacgac cagtttcgca
    52861 ccgacgttga tctcgccttt ttgtacgtga tcggcgcgaa tgttgccgat cccacccgcc
    52921 agcatgatcg gtttgtgata cccgcgcagc tcttcgccgt tgtggctgtt cactttttct
    52981 tcataggtac ggaagtagcc gttcagtgcc ggacgaccaa attcgttgtt aaatgccgcg
    53041 ccgcccagcg ggccttcggt catgatatcc agcgcagtga caatgcgctc aggcttaccg
    53101 aaatcttctt cccacggttg ttcaaagcca ggaatacgca ggttggaaac ggagaaacca
    53161 accaggccag ctttcggctt cgcgccgcgg ccggtggcac cttcatcgcg gatttcaccg
    53221 ccggaaccgg tagctgcgcc cggccacgga gaaatcgccg ttgggtggtt gtgggtttct
    53281 actttcatca ggatgtgcgc cggttcctga tggaagtcgt agcggcccgt ttcgtggtca
    53341 gcaaagtagc ggcccacttc ggaaccttcc attacagcgg cgttatcttt ataagcagag
    53401 agaacgtgat ccggcgtggt ttcgaaggtg tttttgatca tcttaaacag cgatttcggc
    53461 tgctgttcac catcgataac ccagtcggcg ttaaaaattt tgtggcggca gtgctcggag
    53521 ttcgcctggg caaacatata cagttcgatg tcgttcgggt tacgaccaag ctttgtgaac
    53581 gcatcctgca gatagtcaat ttcatcttct gccagtgcca ggccaagacg caggttagcg
    53641 tcgattagag cctgacggcc ctgccccagc aaatcaacgc tggtgaccgg agtcggttga
    53701 tggtgtgcaa acagctgctc tgcatcatct aaagcaaaaa agaccgtttc catcatgcgg
    53761 tcgtgcagtt cagcggtaac ctgctgccat tgttcattgg tcagcgtacc ggcttctata
    53821 tagtaagcaa cgccgcgctc aaggcggttt acctgttgta gaccgcaatt atgggcgata
    53881 tcggtcgctt tcgaagacca gggagagatg gtgccaggac gcggggtcac cagcaggagt
    53941 ttgccttgcg gggcgtggct ggcgagtgcc gggccatatt tcagcaggcg ttcaagttgt
    54001 gcgtgctcat catcgtttaa cggcgcattg aggtcagcaa aatggacata ctcggcgtaa
    54061 atagtgtgaa ccgggagcct ggcagcctga aaacgtgcca gcagtttgtt gattcggaat
    54121 gccgacaatg caggcgaacc acgcagaatt tccatcataa gtctctcgtc ttctaagctt
    54181 tcggtgtacc caaggggggg aaacgggcgt cattataaag aatctgatgc gctgacgaaa
    54241 ccgtttgcgt ggaaataaaa tcaccatcgt gaattagcaa cgcgtgccgc caatggctgt
    54301 aataagttgc catctggcgc aggtttacgc aaaatgccgc tcatttatga gtaaaccttt
    54361 cactattatt acgttttttc aagctgggac gcgcacgaca cagagagtta actaattgaa
    54421 aaaattaaag attaattatc tgttcatcgg cattctggca ctgctgctcg cggtcgctct
    54481 ctggccatcc attccctggt ttggtaaagc cgacaaccgt atcgccgcca ttcaagcgcg
    54541 gggagagttg cgtgtgagca ccattcatac tcccctgact tataacgaaa tcaacgggaa
    54601 accttttggc ctggattacg aactggcgaa acagtttgcc gattacctcg gtgtaaaact
    54661 gaaagtgacc gtgcggcaga atatcagcca gctgtttgac gacctcgata atggtaacgc
    54721 cgacctgctg gcggcaggac tggtctataa cagtgagcgg gtaaaaaatt atcagcctgg
    54781 tcctacctat tattccgtgt cacaacaact ggtttataaa gtcggtcagt atcgcccacg
    54841 tacgctgggc aacctgacgg cggagcaact caccgttgca ccgggtcacg tggtggttaa
    54901 cgatctccag accctgaaag acacaaaata cccggaatta agctggaagg tagacgacaa
    54961 aaaaggctct gcggaattaa tggaagatgt catcgaagga aaactcgatt acaccattgc
    55021 tgattctgtc gccatcagcc tgtttcagcg cgttcacccg gaactcgccg tagcgctcga
    55081 tatcaccgat gaacaaccgg tgacctggtt tagcccgtta gatggcgata ataccctttc
    55141 cgccgccctg ctcgacttct tcaacgaaat gaatgaagac ggtacactgg cacgcattga
    55201 agagaaatac ctggggcatg gcgatgattt tgattacgtc gatacgcgca catttttacg
    55261 cgccgtcgat gcggtactgc cgcagttaaa gcccctgttt gagaaatacg ccgaagaaat
    55321 tgactggcgt ttgctggccg ctattgctta tcaggaatcg cactgggatg cgcaggccac
    55381 ttcaccgacg ggtgtgcgcg gcatgatgat gctaaccaaa aataccgcgc aaagcctcgg
    55441 cattacggat cgtaccgatg ccgaacagag catcagcggc ggcgtgcgtt atttgcagga
    55501 tatgatgagt aaagtgccgg aaagtgtgcc ggagaacgaa cggatttggt ttgccctcgc
    55561 cgcgtacaat atgggctatg cgcatatgct ggatgcccgc gctctgacgg caaaaaccaa
    55621 agggaatcct gacagttggg ctgacgtaaa acagcgtctg cctttactta gccagaaacc
    55681 ctattacagc aagctgactt acggctacgc tcgtgggcat gaagcctacg cttatgtcga
    55741 aaatattcgt aagtatcaga ttagcctggt gggttatctg caagagaaag agaagcaggc
    55801 tacagaagcg gcgatgcaac tggcgcagga ttatccggcg gtatcgccta cggagttggg
    55861 caaagagaaa tttccttttc tctcgtttct ttcccagtcg tcatcaaact atttgaccca
    55921 ctctccctct ctgctgtttt ccagaaaagg gagtgaagag aaacaaaatt aatctgtcga
    55981 ggattgcgct tttttctgcg ctttaatttc ctggcggcgc atgcgaaaga agtcactgag
    56041 caacgccgca cactcatccg ccagtattcc ttccgtaatt tccacccggt gattcatacc
    56101 cggatgatgc agcacatcca ttaaagatcc cgcagcgcca gttttcgcgt cacgcgcacc
    56161 aaagaccacg cgaccaatgc gactgtggat catcgctccg gcacacatta cacagggttc
    56221 aagcgtgaca tacaacgtgg cgtcgatcag acgataattt tgcatcacca gaccgccctg
    56281 ccgtagggcc atgatttctg catgtgcggt gggatcatgt cgaccaatcg gacggttcca
    56341 gccttcgcca ataacacgat tgttatgcac taataccgcg ccgaccggta cttcccgctc
    56401 atcccaggca cgcttcgcca gcgtcattgc atgacgcatc cagtattcgt ggctaaattc
    56461 gacttcagac aaaaagaaaa ctccggttat aaaagcgcgg cgcattatac acggacgcta
    56521 tgctttactc tattccagtt gctggagttc accgcgcgga gttactcgcc aacgatgctg
    56581 acagaaataa agcagcgggt tgtcctgatt actgtcgcta tagccactgt acagccgcag
    56641 cggcgtgccg attttgcgct ccagttgcgc gaccttttca tgtcccagac aacgcatcgt
    56701 caatacccaa ccaccatagc cacgctgaat ctgactggcg ataagattaa cccgcggcag
    56761 ccagggcgta tcgaaataaa ccgcttcaac cagcggctgc ggagagccgg taatcaacca
    56821 gatatcagca tcggaactta acaggtaggt ggttaatcgc tcctgaacca gcggaaaggc
    56881 ggtaacattg tcacgaaacc agcgtacgaa atcggcctgc aacgcctgca aacgcgcttc
    56941 attgtgacca aaagtgcatc cccacagaag cagactcatc ggccagcgtg ccgcacgacc
    57001 ttttatcaat aacgcaatgg ctatgatcgg caacaacggc agaacaagta acgcattcag
    57061 cggttggcga cgcagtaaat aacgcaaaaa actgccgaac atatcctgct gatgcaatgt
    57121 tccatctaag tcaaaaaaca ccacacgacg ctcgtgagtt gccaaaccat actcctctgg
    57181 actgaaacat cctgattaat tttcgctact acatagccta acagatagat catcactttt
    57241 ccggcaacaa tcgaccgcat catggctaac tggaattttt aattcatggc aattagcggc
    57301 aatggaatat aaaattcact cgcgtgtgtc tcatattgcc tgttgtcgcc attatttcat
    57361 cgcgaggaaa gatatgaacg gcttacttcg tatccgtcag cgttaccagg ggcttgccca
    57421 gagtgataaa aaactggcgg attatctgct gctacaacct gatacggcgc gccatttaag
    57481 ctctcagcaa ctggccaacg aagccggagt cagtcagtcc agcgtcgtga agttcgcgca
    57541 aaaactcggc tataaaggtt ttccggcact aaagttggcg ttgagtgaag cgctggcaag
    57601 ccagccggaa tctccctccg tgcccattca taaccaaatc cgcggtgatg atccgttacg
    57661 gctggtcggc gaaaaactga ttaaagaaaa tactgccgct atgtacgcaa cgctaaacgt
    57721 taatactgaa gagaaactgc atgaatgcgt aacaatgttg cgctctgcgc ggcggataat
    57781 tctgaccggt attggcgctt cgggtctggt ggcgcaaaac tttgcctgga agctgatgaa
    57841 gattggcttc aatgctgccg cagtgcgcga tatgcatgcg ctactcgcta cagtacaggc
    57901 gtcgtcccct gacgatctgt tattagccat ttcctacacc ggtgtacgac gcgagttaaa
    57961 cctggcggca gatgagatgc tgcgagtggg cggaaaagtg ctggcgatta ccggctttac
    58021 tcccaatgcc ctgcaacagc gtgcttctca ttgcctgtat accattgccg aagaacaagc
    58081 gacaaacagt gcttcaatct ctgcttgtca cgcgcaggga atgttaacgg atttgctgtt
    58141 cattgcgctg attcagcagg atctggaact ggcaccagaa cgtattcgtc atagcgaagc
    58201 gctggtgaaa aaactggtct gagtaaagaa tgcgcgtata atgcccgccc ggtttgtgtt
    58261 gttttgagag tttccttatg gcgctgttaa tcactaaaaa atgcatcaat tgtgatatgt
    58321 gtgaacccga atgcccgaat gaggcgattt cgatgggtga acatatctac gagattaaca
    58381 gcgataagtg taccgaatgc gtagggcact acgagacacc aacctgccag aaggtgtgtc
    58441 cgatccccaa tactattgtg aaagatccgc aacatgtcga gacggaagaa cagttgtggg
    58501 ataaatttgt gctgatgcac cacgcggata aaatttaata ttctgctctg gaagtagagt
    58561 gttaattatt ttatagaa
//
LOCUS       DAZFFE010000018        54461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFFE010000018 DAZFFE010000000
VERSION     DAZFFE010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.018"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..218
                     /locus_tag="ACHJ69_001286"
     CDS             <1..218
                     /locus_tag="ACHJ69_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014315.1"
                     /translation="KVAMLMQAPYYFQEAQIEAAIATMDVAPEYADIRQVESSTAVLY
                     LFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            282..950
                     /gene="ydhX"
                     /locus_tag="ACHJ69_001287"
     CDS             282..950
                     /gene="ydhX"
                     /locus_tag="ACHJ69_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO5014316.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVIDNDNGTQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAETRLA
                     KGFPPICVSACPEHALIFGHEDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGRHLIK
                     KENV"
     gene            947..1732
                     /gene="phsC"
                     /locus_tag="ACHJ69_001288"
     CDS             947..1732
                     /gene="phsC"
                     /locus_tag="ACHJ69_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO5014317.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPQFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSATDHGERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLDRAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNLLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGEMFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            1736..2548
                     /gene="ydhT"
                     /locus_tag="ACHJ69_001289"
     CDS             1736..2548
                     /gene="ydhT"
                     /locus_tag="ACHJ69_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO5014318.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERLFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGARARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANCGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGCAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(2560..4164)
                     /gene="ydhS"
                     /locus_tag="ACHJ69_001290"
     CDS             complement(2560..4164)
                     /gene="ydhS"
                     /locus_tag="ACHJ69_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO5014319.1"
                     /translation="MKTIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDDENSKLMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMIATNQDLPGETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGILGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVIFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKRENHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(4290..4595)
                     /gene="ydhR"
                     /locus_tag="ACHJ69_001291"
     CDS             complement(4290..4595)
                     /gene="ydhR"
                     /locus_tag="ACHJ69_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO5014320.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFSDEKSALTYLEKHTARLKNLGVEEVVAKVFDVNVSLSQFNQAKLA"
     gene            complement(4703..4779)
                     /locus_tag="ACHJ69_001292"
     tRNA            complement(4703..4779)
                     /locus_tag="ACHJ69_001292"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4743..4745),aa:Val,seq:gac)
     gene            complement(4784..4860)
                     /locus_tag="ACHJ69_001293"
     tRNA            complement(4784..4860)
                     /locus_tag="ACHJ69_001293"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4824..4826),aa:Val,seq:gac)
     gene            5168..6424
                     /gene="ydhQ"
                     /locus_tag="ACHJ69_001294"
     CDS             5168..6424
                     /gene="ydhQ"
                     /locus_tag="ACHJ69_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO5014321.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCAQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKANDTLLAGGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFGIGNINL
                     NGGKLWLITDAATQVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNSLKLQPDQDGIGATLQPA"
     gene            complement(6465..7838)
                     /gene="mdtK"
                     /locus_tag="ACHJ69_001295"
     CDS             complement(6465..7838)
                     /gene="mdtK"
                     /locus_tag="ACHJ69_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO5014322.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRASR"
     gene            8053..8694
                     /gene="ribE"
                     /locus_tag="ACHJ69_001296"
     CDS             8053..8694
                     /gene="ribE"
                     /locus_tag="ACHJ69_001296"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO5014323.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(8734..9882)
                     /gene="cfa"
                     /locus_tag="ACHJ69_001297"
     CDS             complement(8734..9882)
                     /gene="cfa"
                     /locus_tag="ACHJ69_001297"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO5014324.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(10173..11384)
                     /gene="punC"
                     /locus_tag="ACHJ69_001298"
     CDS             complement(10173..11384)
                     /gene="punC"
                     /locus_tag="ACHJ69_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO5014325.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLFIGLTIFALGSLGMLWVENAATLLILRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVGLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            11497..12429
                     /gene="punR"
                     /locus_tag="ACHJ69_001299"
     CDS             11497..12429
                     /gene="punR"
                     /locus_tag="ACHJ69_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO5014326.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(12426..13451)
                     /gene="purR"
                     /locus_tag="ACHJ69_001300"
     CDS             complement(12426..13451)
                     /gene="purR"
                     /locus_tag="ACHJ69_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO5014327.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQSHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            13750..13839
                     /gene="cydH"
                     /locus_tag="ACHJ69_001301"
                     /gene_synonym="ynhF"
     CDS             13750..13839
                     /gene="cydH"
                     /locus_tag="ACHJ69_001301"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO5014328.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            14005..15174
                     /gene="ydhP"
                     /locus_tag="ACHJ69_001302"
     CDS             14005..15174
                     /gene="ydhP"
                     /locus_tag="ACHJ69_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5014329.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPAFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAMSMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPEAVCVANS"
     gene            complement(15320..15901)
                     /gene="sodB"
                     /locus_tag="ACHJ69_001303"
     CDS             complement(15320..15901)
                     /gene="sodB"
                     /locus_tag="ACHJ69_001303"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO5014330.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(16029..16856)
                     /gene="mepH"
                     /locus_tag="ACHJ69_001304"
     CDS             complement(16029..16856)
                     /gene="mepH"
                     /locus_tag="ACHJ69_001304"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO5014331.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTQKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            17191..17538
                     /gene="grxD"
                     /locus_tag="ACHJ69_001305"
     CDS             17191..17538
                     /gene="grxD"
                     /locus_tag="ACHJ69_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO5014332.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(17755..17817)
                     /locus_tag="ACHJ69_001306"
                     /pseudo
     CDS             complement(17755..17817)
                     /locus_tag="ACHJ69_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
     gene            complement(17910..18557)
                     /gene="rnt"
                     /locus_tag="ACHJ69_001307"
     CDS             complement(17910..18557)
                     /gene="rnt"
                     /locus_tag="ACHJ69_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO5014333.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(18660..19067)
                     /gene="gloA"
                     /locus_tag="ACHJ69_001308"
     CDS             complement(18660..19067)
                     /gene="gloA"
                     /locus_tag="ACHJ69_001308"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO5014334.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTNVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(19148..20245)
                     /gene="nemA"
                     /locus_tag="ACHJ69_001309"
     CDS             complement(19148..20245)
                     /gene="nemA"
                     /locus_tag="ACHJ69_001309"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO5014335.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSIPRALELGEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(20282..20881)
                     /gene="nemR"
                     /locus_tag="ACHJ69_001310"
     CDS             complement(20282..20881)
                     /gene="nemR"
                     /locus_tag="ACHJ69_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO5014336.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            20984..21223
                     /gene="ydhL"
                     /locus_tag="ACHJ69_001311"
     CDS             20984..21223
                     /gene="ydhL"
                     /locus_tag="ACHJ69_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO5014337.1"
                     /translation="MTEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRVNKPAPSDEPEQPSLF"
     gene            21272..22168
                     /gene="ydhF"
                     /locus_tag="ACHJ69_001312"
     CDS             21272..22168
                     /gene="ydhF"
                     /locus_tag="ACHJ69_001312"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO5014338.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTACEENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRIRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            22249..22770
                     /gene="sodC"
                     /locus_tag="ACHJ69_001313"
     CDS             22249..22770
                     /gene="sodC"
                     /locus_tag="ACHJ69_001313"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO5014339.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(22771..24783)
                     /gene="ydhK"
                     /locus_tag="ACHJ69_001314"
     CDS             complement(22771..24783)
                     /gene="ydhK"
                     /locus_tag="ACHJ69_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO5014340.1"
                     /translation="MNASSWSLRNFPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWRPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPDATREIL
                     EQLLTALASSQTDVYTVARIISPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMCTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLALA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(24783..25640)
                     /gene="ydhJ"
                     /locus_tag="ACHJ69_001315"
     CDS             complement(24783..25640)
                     /gene="ydhJ"
                     /locus_tag="ACHJ69_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO5014341.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIIELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFTLDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(25643..25879)
                     /gene="ydhI"
                     /locus_tag="ACHJ69_001316"
     CDS             complement(25643..25879)
                     /gene="ydhI"
                     /locus_tag="ACHJ69_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO5014342.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVIHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIVW"
     gene            26080..26514
                     /gene="slyA"
                     /locus_tag="ACHJ69_001317"
     CDS             26080..26514
                     /gene="slyA"
                     /locus_tag="ACHJ69_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO5014343.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(26561..27028)
                     /gene="slyB"
                     /locus_tag="ACHJ69_001318"
     CDS             complement(26561..27028)
                     /gene="slyB"
                     /locus_tag="ACHJ69_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO5014344.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRQVQIQGGDDSNVIGAIGGAVLGGFLGNTIGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTHFSPGQRVVLASNGSQVTVSPR"
     gene            27302..28411
                     /gene="anmK"
                     /locus_tag="ACHJ69_001319"
     CDS             27302..28411
                     /gene="anmK"
                     /locus_tag="ACHJ69_001319"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO5014345.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLCDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            28509..28838
                     /gene="mliC"
                     /locus_tag="ACHJ69_001320"
     CDS             28509..28838
                     /gene="mliC"
                     /locus_tag="ACHJ69_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO5014346.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            28897..29553
                     /gene="pdxH"
                     /locus_tag="ACHJ69_001321"
     CDS             28897..29553
                     /gene="pdxH"
                     /locus_tag="ACHJ69_001321"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO5014347.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHDQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            29682..30956
                     /gene="tyrS"
                     /locus_tag="ACHJ69_001322"
     CDS             29682..30956
                     /gene="tyrS"
                     /locus_tag="ACHJ69_001322"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO5014348.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            31018..31878
                     /gene="pdxY"
                     /locus_tag="ACHJ69_001323"
     CDS             31018..31878
                     /gene="pdxY"
                     /locus_tag="ACHJ69_001323"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HFO5014349.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAN
                     PEHYFSATKL"
     gene            complement(31922..32527)
                     /gene="gstA"
                     /locus_tag="ACHJ69_001324"
     CDS             complement(31922..32527)
                     /gene="gstA"
                     /locus_tag="ACHJ69_001324"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO5014350.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDNYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKSTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(32632..34134)
                     /gene="dtpA"
                     /locus_tag="ACHJ69_001325"
     CDS             complement(32632..34134)
                     /gene="dtpA"
                     /locus_tag="ACHJ69_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO5014351.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLFTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(34745..35380)
                     /gene="nth"
                     /locus_tag="ACHJ69_001326"
     CDS             complement(34745..35380)
                     /gene="nth"
                     /locus_tag="ACHJ69_001326"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO5014352.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(35380..36075)
                     /gene="rsxE"
                     /locus_tag="ACHJ69_001327"
     CDS             complement(35380..36075)
                     /gene="rsxE"
                     /locus_tag="ACHJ69_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO5014353.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDEKMKKRRTEAV
                     AERALPNGETGNV"
     gene            complement(36079..36699)
                     /gene="rsxG"
                     /locus_tag="ACHJ69_001328"
     CDS             complement(36079..36699)
                     /gene="rsxG"
                     /locus_tag="ACHJ69_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO5014354.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDANWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(36703..37761)
                     /gene="rsxD"
                     /locus_tag="ACHJ69_001329"
     CDS             complement(36703..37761)
                     /gene="rsxD"
                     /locus_tag="ACHJ69_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO5014355.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDTIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEEIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPDTLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(37762..39984)
                     /gene="rsxC"
                     /locus_tag="ACHJ69_001330"
     CDS             complement(37762..39984)
                     /gene="rsxC"
                     /locus_tag="ACHJ69_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO5014356.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDNVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKVAVE
                     AAIARAKARKLEHQQANAEPEEQIDPRKAAVEAAIARAKARKLEQQQANAEPEEQIDP
                     RKAAIEAAIARAKARKLEQQQANAEPEEQIDPRKAAVAAAIARVQAKKAAQQKVVNED
                     "
     gene            complement(39977..40555)
                     /gene="rsxB"
                     /locus_tag="ACHJ69_001331"
     CDS             complement(39977..40555)
                     /gene="rsxB"
                     /locus_tag="ACHJ69_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO5014357.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(40555..41136)
                     /gene="rsxA"
                     /locus_tag="ACHJ69_001332"
     CDS             complement(40555..41136)
                     /gene="rsxA"
                     /locus_tag="ACHJ69_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO5014358.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(41213..41653)
                     /gene="ydgK"
                     /locus_tag="ACHJ69_001333"
     CDS             complement(41213..41653)
                     /gene="ydgK"
                     /locus_tag="ACHJ69_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO5014359.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(41739..41954)
                     /gene="ydgT"
                     /locus_tag="ACHJ69_001334"
     CDS             complement(41739..41954)
                     /gene="ydgT"
                     /locus_tag="ACHJ69_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO5014360.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWRYVQ"
     gene            complement(42227..42352)
                     /gene="blr"
                     /locus_tag="ACHJ69_001335"
     CDS             complement(42227..42352)
                     /gene="blr"
                     /locus_tag="ACHJ69_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO5014361.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            42595..43635
                     /gene="ydgJ"
                     /locus_tag="ACHJ69_001336"
     CDS             42595..43635
                     /gene="ydgJ"
                     /locus_tag="ACHJ69_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO5014362.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(43671..44672)
                     /gene="add"
                     /locus_tag="ACHJ69_001337"
     CDS             complement(43671..44672)
                     /gene="add"
                     /locus_tag="ACHJ69_001337"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO5014363.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVADLAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(44776..45948)
                     /gene="malY"
                     /locus_tag="ACHJ69_001338"
     CDS             complement(44776..45948)
                     /gene="malY"
                     /locus_tag="ACHJ69_001338"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO5014364.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLIHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLKAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSGRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKIAIMPGYTYGEEGRGFIRLNAGCPRSKLEKGVAGLINAIRAVG"
     gene            complement(45958..47550)
                     /gene="malX"
                     /locus_tag="ACHJ69_001339"
     CDS             complement(45958..47550)
                     /gene="malX"
                     /locus_tag="ACHJ69_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO5014365.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYIIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMVGLMRTVQA"
     gene            47725..48753
                     /gene="malI"
                     /locus_tag="ACHJ69_001340"
     CDS             47725..48753
                     /gene="malI"
                     /locus_tag="ACHJ69_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO5014366.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRIAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            48865..49632
                     /gene="hdhA"
                     /locus_tag="ACHJ69_001341"
     CDS             48865..49632
                     /gene="hdhA"
                     /locus_tag="ACHJ69_001341"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO5014367.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGKAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            49861..50451
                     /gene="uidR"
                     /locus_tag="ACHJ69_001342"
     CDS             49861..50451
                     /gene="uidR"
                     /locus_tag="ACHJ69_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO5014368.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            50839..52650
                     /gene="uidA"
                     /locus_tag="ACHJ69_001343"
     CDS             50839..52650
                     /gene="uidA"
                     /locus_tag="ACHJ69_001343"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO5014369.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVGANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYLLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNQPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            52647..54020
                     /gene="uidB"
                     /locus_tag="ACHJ69_001344"
     CDS             52647..54020
                     /gene="uidB"
                     /locus_tag="ACHJ69_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO5014370.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQAQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LINDITS"
     gene            54059..54461
                     /locus_tag="ACHJ69_001345"
                     /pseudo
     CDS             54059..54461
                     /locus_tag="ACHJ69_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        13823 a        13344 c        14114 g        13180 t
ORIGIN      
        1 acaaagtcgc catgctgatg caagcgccat attacttcca ggaagcgcag attgaagccg
       61 cgatcgccac gatggatgtt gcacctgaat atgccgatat acggcaggtg gagtcatcga
      121 cggcagtgtt gtacttgttt agcgagcgat ttatgaccta tggcaaggcg tatggcttat
      181 gcgaatggtt cgaggtagag cagtttcaga acccctgaaa tttcaggggg atgtcatgga
      241 tagggtggac agttgcagcc actgcattag gggataatca gatgtccttc actcgacgca
      301 aatttgttct ggggatggga acagtaatct tttttactgg ttctgcgtct tctctgttag
      361 cgaatacgag gcaagaaaag gaagttcggt atgccatgat tcatgacgag tcacggtgca
      421 atggctgtaa tatctgcgcc cgcgcatgcc gtaaaactaa ccacgtcccc gctcagggaa
      481 gccgtttatc gatagcacac attccagtca tcgataacga caatggaacg cagtatcact
      541 tttttcgcca gtcatgtcag cactgcgaag atgctccttg catcgacgtt tgcccgacag
      601 gggcgtcgtg gcgtgatgag caggggatcg tgcgggtaga aaaatcgcag tgcatcggct
      661 gcagttattg cattggcgct tgcccgtacc aggttcgcta ccttaaccct gtcactaaag
      721 tggcagataa atgtgatttc tgcgctgaga cccgactggc aaaaggcttt ccgcccattt
      781 gcgtcagtgc ctgcccggaa cacgcattga tctttggtca cgaagatagc ccagaaattc
      841 aggcgtggtt gcaggaaaat aaatactatc aatatcagct acctggcgcc gggaaaccgc
      901 atctgtatcg ccggttcggt cgacatttga ttaaaaagga aaatgtatga acccgtcgca
      961 acatgctgaa cagtttcaga gccagttggc gaactatgtg ccacagttca ccccccaatt
     1021 ttggccggtg tggttgatca ttgccggact attgctggtt gggatgtggt tggtgctggg
     1081 gctacatgcc ttgcttcgtg ctcgtggcgt gaagaaatca gccaccgatc atggtgagag
     1141 gatttatctt tacagcaaag cggtcagatt atggcactgg tcgaatgcgt tactctttgt
     1201 attgttgctg gcaagtgggc tgataaatca ctttgcgctg gtgggcgcaa ctgcggttaa
     1261 aagtctggtt gcggtgcatg aagtttgcgg atttttgtta ctggcatgct ggcttggctt
     1321 tgttctgatc aatgccgttg ggggtaatgg tcaccactat cgcattcgtc gtcagggttg
     1381 gctggatcga gcggcaaaac aaacgcgatt ttatttgttt ggcattatgc agggggaaga
     1441 acatcctttc ccggcaacaa cccagtctaa atttaatctc ttacagcagg tcgcctatgt
     1501 tggtgtcatg tatggattgc tgccattgtt actattgacg gggctgctgt gtctctatcc
     1561 gcaagccgtg ggagaaatgt ttcctggcgt aagatactgg ttattgcagg cgcattttgc
     1621 tctggcattt ataagcctct tttttatctt cggtcatctt tatctttgca ccacggggcg
     1681 tacgccacac gaaaccttta aaagcatggt cgatggctat caccggcact aacgaatgat
     1741 cattacacga gcagacctgc gagaatggcg tatcggagcg gtgatgtatc gctggtttct
     1801 gcgccatttc ccacgtggcg gtagttatgc cgatattcat catgcactga ttgaggaagg
     1861 atatacggac tgggcggaga gtctggttga gtatgcgtgg aaaaaatggc tggcggatga
     1921 aaacttcgcg catcaggaag tttcttctat gcaaaaactg gccacagatc ccggagaacg
     1981 actattttgc agtcagtttg cccgcagcga cgatcatgct cgcatcggct gttgtgagga
     2041 taacgccaga atcgcaaccg ctgggtatgc cgcgcaaatt gccagtatgg gttatagcgt
     2101 gcggattgga agtgtcggct ttaacagtca cattggcagc tccggtgcac gcgcacgggt
     2161 tgctgtaacc ggtaattctt cgcggataag cagcgctgga gatagcagtc ggattgcgaa
     2221 taccggaatg cgagtacgcg tttgtacatt gggtgaacgg tgtcatgtcg ccagtaatgg
     2281 tgacctggtg caaatcgcca gctttggcgc aaacgccaga attgccaact gcggcgacaa
     2341 tgtacacatc attgccagcg gtgaaaattc aacagtagtg agcacgggcg tggtcgactc
     2401 aattattctc gggccagggg gatgcgccgc actcgcatat catgacggcg aaagagttcg
     2461 cttcgccgtc gccattgaag gtgaaaacaa tattcgagcc ggagtccgct atcgtcttaa
     2521 tgaacaacat cagtttgttg agtgctgata gctttacctt taatcaaacg aaagacgccg
     2581 acgcgcacgg gatgcaggtt ttacgaccgc ccgagccatc gcctctccaa tttctgcaca
     2641 tgccgtaagt ccctgaacga aaggctggtc gtgcatcaac cagggcaacg cgccgaacgc
     2701 tacgcgccca cgaatatctt cgggttgctg taacgtataa tcttcgccaa catcagggat
     2761 ttcatcccct gttttctgta attgttcgcg tagtccaggg aaaggaatat ctttcacttt
     2821 aagcggacgc tgcccgcggg catcaataaa aacgtcaaac gagtaactgt tgtcttccgt
     2881 tttcaggacg gtgcgcgact cgttaatttc catttcgtag tcttcaccga gagcgagaat
     2941 atgaatgatt cccgcttcac gtaaggccaa tagtcgacga atagactctg aagggattgc
     3001 cgcataatta tcgatgaaaa cccgggcaag gcctttactg aaccgtttat ggtcgtgttc
     3061 attcagatgt ggaacaattt cctgtacggc ttcatgcagg cgcagaatga cataacgcca
     3121 gggaacagta tgattttctc gtttattgcg ttcgacctcc tggagatttt tttctgccca
     3181 gtcgaatggg tcgcgttgtt tgcgctcggc aaaccaggct tgagcaaagg aatcgacatt
     3241 caggctttct aaggctatgc gttggctcca gtctggatca gcaaatttga tttcctctac
     3301 tatcaatcta aatacccgat ccaaaaggcc ctcttcgcct ttttgaatct cagcatttaa
     3361 tgcttgatca gtgacgatgt gtaagggctc gtagggaata gggcaataga aatcggcttc
     3421 gggtaaaata cctgtgcgcg acattaacgt aatatttagc ttttcacttg cgttatcgcg
     3481 gtgaaaaatg acgtgttgtt tatcatcttc aatgaacgaa ccatgctgaa tagccactgc
     3541 cattgccgca tccagtccgc tcaaggatgt tcccaaaata cccacgttac acgcatcgac
     3601 ctttgcttcc atcaggcctg accacgggct gggaaaatac gttctggttg cttcttcttc
     3661 atcaggccag acgtgacccg tggcaatcac cgctaaatca aacgtctcgc cgggtaaatc
     3721 ctgatttgta gcgatcatga cgccagcatt tgtaatttgc agatcggtaa cctggcatga
     3781 ttcataaaca gccactgcaa atttttgctg tcgtgcctgg tctactaatc gtaaaaattg
     3841 atcgcggaaa tattcgccca gcagaattcg cggtaaaaac tgacgatcgt gcaaggtttc
     3901 ttttttaacg ccataacgct ggagatggct ggcttcttgc ttttgtagcc attcgagata
     3961 cgtgcaataa atcggcggta tttcaatact ggcaatgttt gccagcatca gttttgagtt
     4021 ttcatcatca ctgtatggca tcccgacacc ggcttcgtca gcctgctcga agatagaaat
     4081 agaaagtggc gtttgttgct gtagaagcga gaataaggtg tagatccccg taggcccggc
     4141 acccacaata gcaattgttt tcattgttac ttacctgtac gcattctttc gaatttagtg
     4201 ttatcaggta agtttggttg agatttttag aatttgagaa atttcagaca gggatgaaaa
     4261 gggcactaac cgcccttgat taaaatctgt caggcaagtt ttgcctgatt gaattgactc
     4321 agtgacacat tgacatcgaa aactttggcg acaacttcct caacgccaag atttttcagt
     4381 cgggcagtat gtttttccag ataggtaagg gcgctttttt catcagagaa aaggtagatc
     4441 ccaccggctt cgtggttctt ttcactttct gtccaaacct tccacagaaa accaggttcc
     4501 tgattaatcg actcagcaag cggtttaagc tgctcagcca ttgcgtcgcc aaatgggcca
     4561 ttaaaagcaa aatgaagttg taacaaagtt gccacagcga ttctccttct gataacgcac
     4621 acgaagacag tgtagatctt ttaacgtatt ggatacagag aagaaacaga gggacaaaga
     4681 agaagaccag attaagaaaa tctggtgcgt ccgagtggac tcgaaccacc gacccccacc
     4741 atgtcaaggt ggtgctctaa ccaactgagc tacggacgca ggatggtgcg ttcaattgga
     4801 ctcgaaccaa cgacccccac catgtcaagg tggtgctcta accaactgag ctatgaacgc
     4861 aacgttgtag gtgacaacgg ggacgaatat tagcggcaga gtgggaaggt ggcaagaggc
     4921 aaaacgtaat tttctgcgct atttcgaccg tttgcagagc ttttaagcaa attggctata
     4981 ttttgttgat ttgcaagggc gatttttatt caggatcgca tttacatctg atacaaccca
     5041 ataatccgtg tatcagagtt taaacagagt tgtttgccac catctcaaat ctgtcgtgga
     5101 taaatccttc gtagccttta cacttaggta gcaataaagg cacacagatg cagcaaagga
     5161 ataaatcatg ggatctgatg cgaaaaactt gatgagcgac gggaacgtgc aaattgttaa
     5221 gaccggcgag gttattggcg cgacgcaact tactgaaggc gagttgattg ttgaagctgg
     5281 cggaagagcc gaaaataccg tggtcacggg ggctggctgg ttgaaagtgg caaccggtgg
     5341 gatagccaaa tgcgcacagt acggtaacaa tggcacgcta tcggtcagcg acggtgccat
     5401 tgccacagat attgttcagt ccgagggagg cgcaattagt ctctctacgc tcgctacggt
     5461 taatggccgc catcccgaag gtgaattcag cgttgataaa ggttatgcct gcggtttgtt
     5521 gctggaaaat ggcggtaacc tgcgtgtact ggaaggccat cgcgcggaaa aaattattct
     5581 cgatcaagag ggtggcctgt tggtcaatgg gacaacctca gcggtcgtgg tagatgaagg
     5641 tggtgaattg ttggtgtatc caggtgggga agccagcaat tgtgagatta atcagggcgg
     5701 cgtttttatg ctggcgggga aagccaatga tacgttgctt gctggtggca ccatgaataa
     5761 tctcggtggt gaagactctg acactattgt tgagaatgga gccatctatc gtctggggac
     5821 ggatggtctt cagctctaca gttccggtaa gacgcaaaac ctgtccgtta atgtgggtgg
     5881 tcgggctgaa gtgcatgctg gtacgctgga aaacgcggta atacaaggtg ggacagtgat
     5941 tttgttgtca cccaccagcg cggacgaaaa ttttgtcgta gaggaagatc gtgcaccggt
     6001 tgaactgacc gggagtgttg cattactgga cggcgcttca atgattattg gctatggcgc
     6061 agatctgcaa caatcaacga ttactgtaca gcagggcggt gtgttgattc tcgacggcag
     6121 tacggtaaaa ggcgacagtg tcacttttgg tattggtaac atcaatctga atggcggaaa
     6181 actgtggctg atcactgatg cggcaacgca ggtgcaactg aaagtgaaac gcctgcgtgg
     6241 agagggagcc atttgcctgc aaaccagtgc gaaagaaatt tcacctgact tcatcaatgt
     6301 gaaaggggaa gttaccggtg atatacacgt tgagataaca gatgccagtc ggcaaactct
     6361 gtgtaactct ctgaaattac agccagacca agatggcatc ggcgcaacgc tccagcctgc
     6421 gtaaaaacac aggcgcaacc cgtgaaggtt gcgccttgtc tttattagcg ggatgctcgt
     6481 tgcagaatga tggctgacgg cagacgttgc aggaaccgca tacgcagcat catcataatg
     6541 gctgccgacg tcaggccaat aataaagcct atccagaagc ctgctggtcc cataggttca
     6601 acgaccagat cggtcaatgc cagaatatag ccgcttggca agcccagcac ccagtaagcc
     6661 gtaaaggtga tatagaaaat ggaacgcgta tctttataac cacgcaaaat tccactgcca
     6721 atcacctgga tagagtcaga aatctgatat accgccgcca gcaacatcaa atgtgcagcc
     6781 agcgttacaa cctcgggatt gtcgttgtac aacagggcga tttgctcccg cagtgaaacc
     6841 gtgaatatgg ccgtcagggt tgccatacag acacccacca taagcccggt ccgcgcagcg
     6901 gtttgcgcat ccagcgttga gccttgaccc agacgataac ctacgcggat ggttaccgct
     6961 gccgccagcg acatgggaag cacgaacatc agtgaactaa agttcagggc aatctgatgt
     7021 cctgcgacat caacaatacc gagcggagac actaacagag ccacgacggc aaacagcgtt
     7081 acttcaaaga atagtgccag cgcaattggc aaaccgagtt gaatcagtcg tttcataacc
     7141 gcaggatcgg gcttcgcggt gcctttttcg ttacgaatat cgcgcatcga gcgggcgcgt
     7201 ttaatgtaag aaaccatggc aaggaacatg acccaataca ccgccgcagt agccacgcca
     7261 caaccaactc caccgagctc aggcataccg aaatgaccat aaataaagat atagttaacc
     7321 ggaatgttca ccagcaggcc gataaagccc atcaccatac ccggcttggt ttttgccaga
     7381 ccttcacact ggttacgggc aacctggaag aaaagataac ccggcgcgcc ccatagcaac
     7441 gcacgcagat aacccacggc tttgtccgcc agagccggat cgatgttttc catggagcgg
     7501 ataatgtagc ctgcattcca cagcaccagc ataatgagaa cggaaacaaa acccgccagc
     7561 cagaaacctt gtcgcacctg atgcgcaatg cgctcacgtc gaccggaacc atttaattgc
     7621 gcgataaccg gcgttaatgc cagcagcagt ccgtgaccaa agaggatcgc cggaagccag
     7681 atagaagtac cgatagcgac cgccgccatg tcggtggcac tatagccgcc cgccatcacg
     7741 gtatcgacaa aacccatcgc agtttgggcg atttgcgcga gaatcaccgg gattgctaat
     7801 gctaataaca gacgcgcttc actgatatac ttctgcacgt gaacaccttt tatttgtagt
     7861 tatatgagag actaaaaagc cgcctgaatg ggcagcaaaa agaagaagca ggggaaattc
     7921 agtctattgt agcgaggatt tattaattct ccagtgaaaa aatagccatt cttacccctt
     7981 cactggtaag ggcgttttcc acctgctatt gtgctgaaca gaatgtctta actgatttca
     8041 ggagttgtaa gtatgtttac ggggattgta cagggcaccg caaaactggt gtcgattgac
     8101 gagaaaccaa attttcgcac gcatgtggtg gagttacccg accacatgct agacggtctg
     8161 gaaaccggtg cttccgtggc gcataacggt tgctgcctga ccgtgacgga aattaacggc
     8221 aaccatgtca gttttgacct gatgaaagaa acgttacgca ttactaatct tggcgattta
     8281 aaagtggggg attgggtaaa cgttgagcgt gcggcgaaat tcagcgatga aattggcgga
     8341 cacttaatgt caggtcatat tatgaccacc gctgaagtag cgaaaatatt aacctcggaa
     8401 aataatcgtc agatctggtt taaagtccag gatagtcagt tgatgaaata tatcctgtac
     8461 aaaggattta ttggcatcga cggtattagc ctgaccgtcg gcgaagtcac gccaacgcgt
     8521 ttttgcgtcc atttaattcc ggaaacactg gaacgcacga ctcttgggaa gaaaaaactc
     8581 ggcgcacgcg tcaacattga aatcgatcca caaactcagg cagtggtaga tacggtagaa
     8641 cgtgtgctgg cggcacgaga aaatgccatg aatcaaccag gtacagaagc ctgatctgaa
     8701 atcgcccccg gagcgggggc gatagaatag cctctagcga gcgactcgaa ggccgttttc
     8761 gactccgcga gagaacacca cctgccagag ctgaatatca cgggcgcgga aagcacctgc
     8821 acaggcattc agataatagg taaacatccg tttaaagcgt tcactgtagt tatccgcaat
     8881 ttctggccat gcggcgagga atcgttcata ccacgccatc aacgtagtat cgtaatcagc
     8941 accgaagtta tgccagtctt ccatcacaaa gtggggttcg ctggactgag ctatctggcg
     9001 tacagagggc aggcaaccgt tcggaaaaat atatttatta atccagggat caacattcag
     9061 atcggttttt ttcgaaccga tggtatggag caggaatata ccttccggtt tcaaattacg
     9121 atccaccacc gcaaaatagg tatcgtaatt tttcggtccg acgtgctcga acatccccac
     9181 agaaacaata cgatcaaact ggtcgttcag gtcacgataa tcttgcagca aaatggtgac
     9241 atccaggcct tcacagcgtt cctgagccat tttttgctgt tcggcagaaa tggtgacgcc
     9301 gaccacgctt acgtcataat tagacgccat gtagtgcgcc agtccacccc agccacagcc
     9361 aatatccagt acgcgcatcc ctggttttaa ctgcaatttt tcacaaatca ttttgagctt
     9421 cgcctgctgg gcagattcca gattatcggc atctttccag taagcacagg aatattgcat
     9481 gaagggatca agcatgcggc tgaacaagtc attacccaaa tcgtaatgct ctttgccgac
     9541 tatccaggca cgttttttac tctgcagatt gaagagacga gcgccggcaa tacgcagcgt
     9601 gtctttgaaa tgatggggaa gttggttctc gagacctgcg cgtaagactt tgctaaaaaa
     9661 catatccagt cggtcacatt cccaccagcc atccatataa ctttcgccta accccaaaga
     9721 accttcctgc agaacgcgtt taaaaaaatc ggggtttttg acacgaatat ccgccggggc
     9781 agaaccgtta atggctatac cggcacggct aagtaattcg ttggcgatac ggtaccagtt
     9841 atcatccggt acactgactt cttctataca cgatgaactc atagtttctc catcactggt
     9901 cggtgatcag aaccttaaaa cagcgtagac gctttttggg ctttgtgaga aatctcgcag
     9961 aaaaaaccgc gagtctataa tccgacgtag aacagaggga aaggaggaac ccttgccgaa
    10021 atgaccatcc atagtgagtc gggaacgatc ctgtccccgt aataacataa tatttatcgt
    10081 agttttaaca actcgaaatt agtataggcc gctaatttca atcatacaac aaaattttgt
    10141 tagcagccta agtataagta tatcgatata gatcagtgtg attcgctatg agcgacttcg
    10201 gcattgccat gattctggca gccaacttct tcacaacgtt gcatcatgta acccagagcg
    10261 accagcacta ctgttgataa catcacgctg gtggtggtga gcaatggcgt gctgatactg
    10321 ataagccagg aaactaccag acttgcgagg aagcacagac ccagttgcag agtgttctgc
    10381 aacgctgcgg cgcgaccggt tgcatgtggg aagggacgca gcgcctgggc gacaacaatc
    10441 gggtagatcg caccgttggc aatcgccatc acacagaatg ggatcaggat ttcgaccagc
    10501 gacacatggc taatgaagcc cgcagcccag gtcgcaatga cgctgacagc aaacagtacc
    10561 agcaaccacg gtaataactg cttgccttgc catttctgca gcgcggcgcg acagccataa
    10621 ccaccaatca gaaacgcgat agtttgcggg acgtaactta aaccaataac tgccgggctg
    10681 taacccattt cactaaggat gaacggtgaa ccggtcagcc atgcaaaaaa actggctgaa
    10741 caggccgcgt atatcagcac gttgccgcga taggttttag aacgtagcag gtcggtaaag
    10801 gtcagaccat cctgactatt gttacgggcc ttcgtcgtgg gtttgagcca gaaaataggc
    10861 agaatcagcc ccacggtaat ggcaaacagg gtggcgaaaa tcgcctgcca ggaaaaatgg
    10921 accagcagcc agcttcctaa cagaggagcc agtgccggag acagacccac cagcggcatg
    10981 atggtcgcaa aaatacggtt aactttctgt gaaggataat aatctgtcac taatgcttgc
    11041 cagataaccg ccgcggcgca gacacccaca gcctgtacaa aacgcaatat cagcagcgtg
    11101 gcggcgtttt ctacccacag catccccaga ctacctaacg caaaaattgt caggccgatg
    11161 aataacaccg gtttacgacc ataacggtcg gagagtggcc cccacagaag ctgggctgcg
    11221 gcaaaaccgg caaggaacag actaaggctg gcactgacag cagacgcagg cgtttgcagg
    11281 tcggcctgta tggcggcgaa agcaggcaga tacatatcgg ttgccagaaa accgagtacg
    11341 ctcaaacccg ccagccagac taaaaatctt ttcccaggtt gcatgtatat tctctaaatc
    11401 tctcaattaa acaccgccgc aaagtgtagg gagtgatatt agtgttgtga aacggtaata
    11461 tttgatgatt ggtttcaaaa attttgcagg cagactatgt ggtcagaata ttctctggaa
    11521 gttgttgatg cggtagcgcg taatggtagt tttagcgccg cagcccagga gttgcatcgc
    11581 gtcccttctg cggtcagcta taccgtgcgt cagctggaag agtggctggc ggtgccgctt
    11641 tttgaacgac gtcaccgtga tgtggaactg accgctgccg gggcgtggtt tctcaaagaa
    11701 ggacgctctg ttgtcaaaaa aatgcagatc acccgccagc aatgtcagca gatagcgaac
    11761 ggctggcgcg gtcagttagc tatcgcagtg gataatattg tcaggccaga gcgaacacgg
    11821 cagatgatcg ttgattttta tcgccatttt gatgatgtcg aacttcttgt ctttcaggaa
    11881 gtgttcaacg gtgtctggga tgcactttcc gacgggcgcg tggaactggc gattggcgca
    11941 acacgcgcga ttccggtagg cggtcgttat gccttccggg atatggggat gttaagctgg
    12001 agttgcgttg ttgccagcca ccacccgctg gcgttgatgg atggcccgtt cagcgatgat
    12061 acgttgcgca actggccgtc attggtgcgc gaagacacct cgcgaacgct acctaaacgt
    12121 attacctggt tgctggataa ccaaaaaaga cttgtcgtgc ctgactggga atcatcggca
    12181 acctgtatct cggcaggatt atgcataggg atggtgccaa cacatttcgc caaaccgtgg
    12241 cttaatgagg ggaagtgggt agcactagaa ctggaaaacc ccttcccgga ttcggcatgt
    12301 tgtctgacat ggcagcaaaa tgatatgtcg cctgcgttaa cctggttgct tgagtatctc
    12361 ggcgatagtg agacgctgaa taaggagtgg ctgcgggagc cggaagagac tcccgcaacg
    12421 ggtgattaac gacgatagtc gcggaacggg ccgtcagcca cggagcggcg ttcaatcaac
    12481 cgcggatgca cttcaatgga ctgcggttct tcacgtttgt tgacgatacg atccaacagc
    12541 atgttgaacg ctgtttcgcc cagcgaatct tttggctgat ggatcgtggt cagcgccggc
    12601 gtaaaatagc gggcgttgcg cacgttatca taaccgatca gcgaaacatc ctgcggcacg
    12661 cgcagaccca tttcatcagc agcacaaagt gcgcccattg ccatgatatc gccaccacag
    12721 aagacggcag tagggcgatg cgactgcgac aggatttgct gcatggcgcg ataaccggat
    12781 tcaggttcaa agtcaccctg cacaatccag ctttccggca ctttgatcat cgcttcttcc
    12841 atcgccttca taaaaccggc aaggcggcct gcgccggtgt tacgttccag cgggccaggg
    12901 ataacgccga tttcgcgatg accgcgttca atcagataac gcccggccat gtagccgcct
    12961 tcgaatgcgt tatcaatgac cgcatcggtg aagtcagctt ttgcttcacc ccagtccatc
    13021 accaccattg ggatatggcg atactcttcc agcatcgcca gcaacggctc tgggtactca
    13081 gaacacatca ccagcagacc atcgacgcgt ttttgcgcca tcatcgacag ataagcccgc
    13141 tgtttctcaa gattgttcca cgcattgccc agaatcaggg tgtaaccttt ctggaagcaa
    13201 tttttttcga ccgcttcaat gatctcggca aaataggccg cttcgctgct ggtcgccagc
    13261 aaaccgatag acttggtgtg gttaaccttc aggctacgcg ccaccgcgct aggggagtag
    13321 tgtaattctt tgattgctgc ccacacggca ttgcgcgttt cttcagcgac gaaacgtgtt
    13381 ttgttgatca cgtgtgacac agttgtagtg gaaacgtttg ctcgtttcgc tacatctttt
    13441 attgttgcca ttccatttca ctccagaccc taacttcagc tcctgcaaat tcgcaaggta
    13501 aacgtttgcc tttacacacc ttacgcacaa caaaaatgtt gcggtacgcc ggaaaaacga
    13561 cacggagcgt caggaagggg tcaatggcca gtacgcaaat aatgtaagcg tggaattttg
    13621 gccgatcttg acgaaaaggg gaagtagtaa aaacacttat cattataaat gacgggagat
    13681 tttttcatca cagtgtgtaa aaatgcgtaa tatcactttt tgtgtggtaa taaaaaagga
    13741 gaaaacttga tgagcaccga ccttaaattt tcactggtaa caacgattat cgtcctcggt
    13801 ttgatcgtgg ccgtgggttt gactgccgcg ctgcactgat ttcctctcga gggagcgcgt
    13861 tctccctcgc atctccttcc tgtttgttag caaaatggca aaagtgtttt ctctttttgt
    13921 tattttcatt tttttgtgat tactgtcatg ctttcgccac tttaatgaat atcgcgtaat
    13981 gacgcgaaga ggtggagata aggcatgaaa attaactatc cgttgctagc gctggcgatt
    14041 ggcgcgtttg gtatcgggac aacggagttc tcgccaatgg gcttgttgcc cgtcattgcg
    14101 cgcggtgtgg atgtctcgat tcccgctgcc ggaatgttaa tcagtgctta tgcagttggc
    14161 gtaatggttg gcgcgccgct gatgacgctt ctactttctc atcgtgcccg ccgcagtgcg
    14221 ttgattttcc tgatggcgat tttcacgctt ggcaacgtac tttccgccat cgcgccggat
    14281 tatatgactc tgatgctttc acgcattttg accagcctga atcacggagc attttttggt
    14341 ttgggttcag tcgtggccgc aagcgtagtg ccaaaacata aacaggctag cgcagttgcc
    14401 actatgttta tggggttaac cctggcaaat atcggtggcg tgccggcggc gacctggttg
    14461 ggcgaaacca tcggctggcg gatgtcattt ctggcaacag cggggctggg cgtgatttca
    14521 atggtaagtc tgttcttctc attacctaaa ggtggcgcag gggcgcgacc tgaagtgaaa
    14581 aaagagctgg cggtattaat gcgtccgcag gtgctgtctg cattgctgac gacggtactg
    14641 ggcgctggtg caatgtttac cctctacacc tatatctctc cggtactgca aagtattacc
    14701 cacgccacac cggcgttcgt cacggcaatg ctggtgctga ttggtgtcgg attttctatc
    14761 ggtaactatc tcggcggcaa actggcagat cgttcagtta acggcacgtt gaaaggcttt
    14821 ttgttgttgc tgatggtgat tatgctggca atcccgttcc tggcccgcaa tgagttcggc
    14881 gcagctatga gcatggtggt gtggggagct gcaacctttg cggtcgtacc gccgttacag
    14941 atgcgcgtga tgcgtgtcgc cagtgaagcg ccaggtctgt cttcatcagt caatattggt
    15001 gcctttaatc ttggaaatgc gctgggagcc gctgctggtg gtgcggtaat ttccgctggg
    15061 ctgggataca gttttgtgcc ggtgatgggg gcgattgtcg cgggactggc attattgctg
    15121 gtgtttatgt cagccagaaa acaacctgaa gcagtttgcg ttgccaacag ctaattaaac
    15181 aaaaacgcag aaggataacc ttctgcgtta cagttgctgg caacgtgcga tttatcttgc
    15241 cgaatgcgct gttaacgcct catccgcgtt ggcatggtaa gcgaagcgca tcagataatg
    15301 ttgcatttgc catcagttat tatgcagcga gatttttcgc tacgaattcc cagttcacca
    15361 gcgcccagaa gtgctccaga tagccaggac gtgcattgcg atagtcgatg taataagcgt
    15421 gttcccagac atcaacggtc agcagcggag tcgcatcggt ggtcagtgga gtacccgcgt
    15481 tagaggttga aacgatagcc agtttgccat cgctgttttt caccagccag gtccagccag
    15541 aaccaaagtt tttgatcgct gcatcagtaa actgagcttt gaaatcggca aagctgccaa
    15601 aagatgcggc gatagcttca gcgacttttc cagtcggttc gccacccgcg ttcggtgcca
    15661 ggcagttcca gtagaaagta tggttccaga cctgagctgc gttgttgaat acgccacctt
    15721 cagagctgcg aataatctct tccagtgatt taccttcaaa cgcggtacct ttgatcaggt
    15781 tgttcaggtt agtgacataa gtctgatgat gcttgccgta gtgatactcg atggtttccg
    15841 cagaaatgtg cggtgccaga gcatctttag catatggtag tgcaggtaat tcgaatgaca
    15901 ttgctactct cctttattat taatttgcat acgtacaata gccttattgt gcgtatggat
    15961 agggtagcaa attaaaaggt gagacaaaag atgaacttac cctgttgccg taacaacagg
    16021 gtaaagtttt agcgaagtgt ttttggggtc attacccggc gagcgccaac atagtggcgc
    16081 tgccagtagt cttcactgag agaagtgatt tgaatttctt ggcccgtgcg cggtgactga
    16141 ataaatttgc cgttgccgac atacacgccg acatgatcgg ctgtaccgcg tccctgagta
    16201 cggaagaaga ccaggtcgcc gtttttcagt tcactacgtt cgattggcgc tgcatcgcgc
    16261 aggtgataca tttcattcgc cgtacgcgga atacgaattt tcaccaaatc tttataagcg
    16321 taataaacca gaccgctgca atcaaaaccg gtacgcggtg agctgccacc ccaacgatat
    16381 ggcttaccaa tttgctgcat cagtttattc attgccactt ttgtggcttt ttgcaccttc
    16441 gctttgtgcg cgtcggcaag ggttcctgac gcggcatttt tgactttcac acaatgcgat
    16501 ttatagccct tacgtttggt acacttttcg gtaaagctgg ctgaggccgt tttattgacg
    16561 gcgtgtttgc tgcgagtttt ggcgttttta gaagcggtaa tggaagattt tttaacggaa
    16621 gaagcggttt tgcttttggt cgtactttta ctggcggctt ttttcgctgt tttctggctg
    16681 gctttctggg tcttttgggt ggttgccgtg cttttctttt tatctgcttt tttggtgata
    16741 tgagtggtag cagaactctc cctggcttgc tttgaagcat gggccatagg cgttaaaggc
    16801 agggtggtaa aaagtaaagc acagagcgtg atcgaaatac ggtttatccg cgccactgag
    16861 cgtccctctg ttaaggcagg tctatcatgc ctgcgtctga tttacaaaac ttgatgattc
    16921 taatgcatag aagaagagaa agttaatagc ttttattgtt aaaaaatacg tttcgtcggc
    16981 gagcgcaaaa aaatgcagca gtgaaagcta aaaatggtta taaatacgcc agtttgccgt
    17041 gcatcaggga agaaacgaat atttgaaatg caacaaatag agcaaaggga taaaatagca
    17101 aaagcgtgat gaaaatgtta ttttccgatg ccgcactgac ccgctacaat gtcagattac
    17161 tgccgtaaag aagtttgagg aagcaagaca atgagcacca ctatcgaaaa aatccaacgc
    17221 cagattgctg aaaacccgat cctgctgtac atgaaaggtt caccgaaact gccgagctgc
    17281 ggtttctctg cccaggcagt ccaggcgctt gccgcatgtg gcgaacgttt tgcctatgtt
    17341 gatattctgc agaatccgga cattcgtgcg gaactgccga aatatgcgaa ctggccgacc
    17401 ttcccgcaac tgtgggttga cggcgagctg gtgggcggtt gtgatatcgt gatcgaaatg
    17461 tatcagcgtg gtgaactgca gcagctgatc aaagaaactg ccgctaaata caagtctgaa
    17521 gagccggacg cggaataatc cgtcctgcaa tactgacgga tgcgaacggg catccgtcag
    17581 tcctttctct atccccaatc cagcccttgt ggcgaacttg aaaatcccgc ctcacgtaac
    17641 cgcgtaaaca tcggcgtttg ccggaccggt aggtcattca catctgttag cgtaaagcgc
    17701 agccgtggtt cgcgactgga cagctgtcgg ctgtttaaag gcgcgaagaa actagtcgcg
    17761 ggtcgccggt gaaaacacgt ccggcagggg cgatgaaggg tttagattat ctgccattaa
    17821 cgctattccc cgtgaatatc atgcatgatg cgtggaatag cctcactata gatgcctgaa
    17881 tcattgcatg tcatcaggca tcgactcgat tacacctctt cggcggcagg tagcggccag
    17941 cctcccagac gtttccagcg gttgacgatt tcacaaaaca gcacagcagt gcgttcggtg
    18001 tcgtacaacg cggagtgcgc ctgggtgctg tcgaagtcca tgccagcggt ctggcaagcc
    18061 tttgacaata cggtttgtcc gagtgccagc ccggccagtg cagcagtgtc aaaagtggcg
    18121 aaagggtgga acgggttacg tttcagtgag gcgcgttctg cggcggccat cataaagctg
    18181 tgatcgaaat tggcgttgtg cgccaccata atggcgcggt tacagccgct cgctttaata
    18241 cctttacgta caactttaaa aatttcgtgc agcgcctcgt actcgctgac cgcgccgcga
    18301 tcgggatcgt tcggatcaat gccattgaag gcaagggcct ctggttgcaa atttgcgccg
    18361 acaaatggtt cgacgtggaa atgtaatgtg gtgtccggca tcagccagcc ttgttcatcc
    18421 attttcaggg tgatggcggc aatctcaagc agcgcatcgg ttttggcgtt aaatccggct
    18481 gtttcaacat cgatcacaac aggataaaaa ccacgaaaac ggtcgcacag accggtaagt
    18541 tgagcgttat cggacatctg attctcttaa tacggataaa ttgcagcgcg cattatgaca
    18601 aatattactt gtcgatgcag taaagatgcg ggcgcgatga gttcacgccc ggcaggagat
    18661 tagttgccca gaccgcgacc ggcgtctttc tcttcgatta attcaatttt gtaaccgtcc
    18721 ggatcttcca caaatgcgat aaccgtagta ccgcctttta ccggacccgc ttcacgggtt
    18781 acgttacccc cgttttgacg gattttttcg cacgcttcag cggcgttatc tacgctaagc
    18841 gcgatgtgac cataagcagt gccgagttcg tatttatcca cgccccagtt gtaggtcagt
    18901 tcaatcactg cttcttcggt ttccgggccg taaccaacaa acgccagtga gtatttgtat
    18961 tccgggtttt cgctggtacg cagcagtttc atgcccagca cgttggtata aaaatcgatg
    19021 gagcgttgca gatcgccaac gcgcagcatg gtatgaagaa gacgcatctt tatatcctca
    19081 aaatggctaa cagattcaaa aagttgtttt agtatagcgg cggctttgcg ccgctcgcaa
    19141 tgttggatta cagcgtcggg taatcagtgt aaccttcagc accgccacca taaaaacttt
    19201 cggcacgctg cgggttaagc tcagctttgc gctgcaagcg ggcgaccaga tccgggttcg
    19261 caatccagtc acgaccaaat gctaccgcat caattaaccc tttgccgatc agcgtttcag
    19321 ctttttctac tgtgtatgca cctgcgccga taatcggacc gtggaaacgg gcgcgtactt
    19381 tttcgcgaaa cgcatcagta tatggttcac ctcccgccca atctggttct gacatatgaa
    19441 gataagcaat gccgcgttta cccagttgtt caatcagata cagtgcgtcg gcttcttcat
    19501 tcgggccgtt atccgtgttc tggaaagtac cgattggcga aatgcgaatg ccaatgcgat
    19561 cggcacccca ttcttcaatc ccggcatcga ccacttccag caccagacgc gcgcgatttt
    19621 ccacgctgcc gccgtactga tcggtacgat ggtttgaaga aggggaaagg aactgatgca
    19681 gcaaataacc gtgagcagag tggagctcta ccagatcaaa accggcttca cgcgcgttag
    19741 caatggcctg acggaaatca ttgacgatac ccggaatctc tcccaattca agcgcacgcg
    19801 gtatggatgt ttcaacacgg atcgcctgac cattttcatc gcgcagagaa gtacgtgttc
    19861 ccgcgctaag tgctgaaggc gctaccggtg cctgaccgcc aggttgcagg ctggcgtgag
    19921 aaatgcgtcc ggtgtgccac agctgtacgg ccatatgacc attttcagca tgaacgccag
    19981 cggtaatttt tttccatgcg gcaatttgtt ccgggctatg gatgccaggt gcacctgcgt
    20041 atccttttgc ctgggcagaa atttgcgtgg cttcgctaat aatcaaaccg gcactggcac
    20101 gttggcgata gtattccgcc atcaacgggg tagggatgtc acccggttca atactgcgca
    20161 gacgcgtcag cggtgccata aaaatacgat ttgccgccgt gatcgcgccc actttcagtg
    20221 gggaatacag tttttcagat gacataaagg ctcccggtcg tctggtgata aaaaatgcct
    20281 gctaaacggc aggcgtcgca ataatgtttt ttacatgggc cagcgcgttt tccagtggct
    20341 cgaaactgcg cgaaattttg gcctgcagat tcgcgccaag ccacagtgcg taaagcactt
    20401 gtgcctgttg cagcggttcg ccacaaaagg ttaaacaatg gttctcacgg ccattttcca
    20461 gcgcctgaga gagcagggcg atcacgccgc gagccccttt atccattgcg ctgcgcatat
    20521 cttctgacag atcgcacact tcggcagaga gttttactgt caggcaacca ctgatggttc
    20581 catgttggca aaactggttc agtgtttgct ggtaataagc cagtatgcgg tcgcggtagt
    20641 taccttcgcc ggattgcagc aactcagtca gtcgctggtg atatgcggcg taatggcgct
    20701 caagcatagc aacgccaaac gcttctttag aacgaaagta gtgatagaag gaccctttcg
    20761 gcacttcagc ggtttttagg agttcgctta gccccatccc ggtgaatcca cgttgcaggc
    20821 aaagttgctc gcccgtcgcc aggagatgtt cgcgagtatc atgttcggtg tgtttgttca
    20881 tgcgttggag tgtagtagac cagtcggtct agtgcaagca aagttgctac tttggacatt
    20941 aacagcttca ataatggtaa taatcttatc tgaggagggc gaggtgacgg agcaattaga
    21001 gttctttccc gtccagagcc cgtgccgggg aatttgccag tctgacgaac ggggattttg
    21061 tcgcggctgt tttcgtagcc gggatgagcg ttttaactgg aataagatga gcgatggcga
    21121 aaaacaagaa gtgttgcgcc tttgtcggca gcgattaatg cgcaaattac gtgtaaataa
    21181 gccagcgcca tcagacgaac cggagcaacc atcactcttt tgagcctaat cttgcgtata
    21241 ctcctctcat atctgataag aggaagcgat tatggttcag cgtattacta ttgcgccgca
    21301 aggcccggag ttttcccgtt ttgtgatggg ctactggcga ttgatggact ggaacatgtc
    21361 cgcccgccag ttggtcagtt ttattgaaga gcatctggat ctcggcgtga ccaccgtgga
    21421 ccatgctgat atttatggtg gctatcagtg cgaagcggcg tttggcgagg cgctgaaact
    21481 ggcacctcac ctgcgtgaac ggatggaaat cgtcagtaaa tgcggtatcg cgacgaccgc
    21541 gtgtgaagaa aacgtcattg gccattacat cactgaccgc gatcacatca ttaagagcgc
    21601 cgaacagtcg ctaattaatc tcgcgaccga tcatctggat ctgctgttaa tccaccgacc
    21661 agacccgtta atggatgccg atgaagtggc ggacgccttt aaacatctgc atcagagcgg
    21721 caaagtgcgt cattttggtg tatcaaactt tacacctgcg caatttgcgc tgttgcaatc
    21781 acgcctgcca tttactcttg ccactaatca ggtggaaata tccccggtgc atcagccgtt
    21841 actgctggat ggcacgctcg accaactgca acaactgcgt attcgtccga tggcgtggtc
    21901 ctgccttggt ggtggtcgtc tgtttaatga tgattatttc cagccgctgc gtgatgaact
    21961 ggctgtggtg gcagaggagt taaacgcggg ctcgattgaa caggtagttt acgcctgggt
    22021 actacgttta ccgtcgcagc cgctgccaat tatcggctca ggtaaaattg agcgcgtacg
    22081 ggcagctgtc gaagcagaaa cactgaaaat gacccgtcaa caatggtttc gtatccgtaa
    22141 agcggcacta ggttacgacg taccgtaagc acttttagga atagccgccg ttcaaaaatg
    22201 tgtcactggt ttacacttat tcaggaatgc acaatgaacg gaggtcctat gaaacgtttt
    22261 agtctggcca ttctggcgct ggttgttgcg accggcgcac aagctgccag tgaaaaagtc
    22321 gagatgaacc tcgtcacgtc gcaaggggtt gggcagtcaa ttggtagcgt caccattact
    22381 gaaaccgata aaggtctgga gttttcgccc gatctgaaag cattacctcc cggtgaacat
    22441 ggcttccata ttcatgccaa aggaagctgc cagccagcca ccaaagatgg caaagccagc
    22501 gccgcagaat ccgctggcgg gcatcttgat ccacaaaata ccggtaaaca tgaagggcca
    22561 gaaggtgccg ggcatttagg cgatctgcct gcactggtag tcaataatga cggcaaggcg
    22621 accgatgccg tcatcgcgcc tcgtctgaaa tcactggatg aagtcaaaga caaagcgctg
    22681 atggtccacg ttggcggcga taatatgtcc gatcaaccca aaccgctggg cggtggcggt
    22741 gaacgctatg cctgtggtgt aattaagtaa ttaagaagcc agcgtacctt gcggcggtgc
    22801 ttgctcaagt tgcgaaagcg agcagtacag ccgccagaca attgccgcca gttctcgggc
    22861 ggcaggttga tgatgacggg caaggctgtc gcaaattcgc tgtaattctt caagtgtggc
    22921 cgccagtgat ttttgctgaa cgccacgctc actcatcact ccccgcaaca gtgaaataca
    22981 gttatcccgt actcgcgata acggatcgga acgcgattcc cagtcgcgca attgccagac
    23041 aacatgagaa cagttcagca acactacacc ccagcgtaat aaccaacggc gagccagcgc
    23101 atcctggctg ttactcaact gactgacgtg atgataagtg agcgattcaa attcgctttc
    23161 actcagtgtt ggatggcggc ttagttgatc gacaaaatcc cggcgtagcg cgcgaatatg
    23221 gcggcggctt ttacgagcat ccgatcctgg acgcagaatg gcgagcgcta accacgccaa
    23281 cgcgacgcca acaatttttg ccagattatc gttaagaaaa tcagcaaaat catacaccgg
    23341 gggattagtg acagcgataa acgaacccat aaaaacaatc agttgccccc acagtgcggc
    23401 aaatttgggc atctgcaatt taagaagctg cattgtcgcc agcagtggaa agagaaacag
    23461 taaaaattgc cacagatcgc taatctgcac catcaggcca aatttgacca caaagctgaa
    23521 tagcgaaagt aacaccagcg tgcacatcag cagtgacaac gacttaaacg gtgccgcgac
    23581 ggcggagtag agtacgcagc taattgctgc cagcgttaaa gcattggcac cggcatccca
    23641 ttgtgaagca atactccatg cgccaatcat catcaacgta caaaatgtac gcagcccgct
    23701 ccacatagct tcggcgttat cggtatgacg agccaggccg gatgtccgtg ggagtctggc
    23761 atgatcatct acaccgcttt gcagacgatg taattcctga ctactttgca gataaaggcg
    23821 gcaaaaataa cgtagtcgct gccagaaggc gacgtgccgg tagtcggcga cgttggtcgg
    23881 gcgtagcggg gagataatac gtgcgacggt gtaaacatct gtttgcgaac tggcgagcgc
    23941 cgtcagcagc tgttcgagaa tttctcgcgt ggcatctggc ggtgagggcc agttgagcaa
    24001 catacgtcgc aggctggaga tgacactggt catacgtaat tgctggtgga gtagcgcatt
    24061 aaggcgcgcg ttttgctggc gaaaacgata gtggctccag aaagcctgga tacgcagcaa
    24121 attcatggtc agtatctgcc caatcacccc ttcatgtgct gcacgaatgg catcggttgt
    24181 ttcaggccgc cagagtaaac tggcatgttc cagtaatcgg gcgtgcatgt ttttcaatgc
    24241 ggttaaaagg gctgtagcat cggaactgct cggcaggatc atcatcatca tgccgccgca
    24301 caaaataccg actatcacct cgcaaacacg cgcctgagcg atatcccaca gctggctggc
    24361 ctcagtaata ttaaccatcg gaaaggcgat gatggcagcc gtgtagcccg ccagttgaaa
    24421 tgcatacgcg acgttattcg tgaagtgcgc acaggcccag gtacaaaagc caagccacgc
    24481 cgacatgctc aatagaaaaa accacggctc attgagcgta tgtccggcaa gaagcagtgc
    24541 cgcaatggct ccgagcaaac tgccagcgat gcgtccgagg cttttgctga tgacaccgcc
    24601 aacggtggga aagctaacca ccgcagccga ggtcatcgcc caatagggct catcaagatt
    24661 caaataatag gcaaccgtca gcgccagaca catggcaatg gtattgcgta acgcataacg
    24721 ccattgcgcc agcgtggccc tgaaccaggg aaaattgcgc aaggaccatg acgatgcgtt
    24781 cattattgac ctacggcaat gctacaggtg gtgcccgaca ccagcgtgac atcgcctggg
    24841 actttatcca gcgtaaagcg gacgggaacg cgttgggcga ggcgaaccca ggggacgtta
    24901 ggtttcacat ccggaataag gcttgagtca ctttcaacgc tctgatcata aatcgcccga
    24961 ccgatactgg acacgtgacc ctgtaacgtt ttgttatcac tatagagggt aatttgtgct
    25021 ggtgcacctt cgcggatatg gcgcaacttg gtttcttcaa aataaccaat gacgtaaaac
    25081 gagtggctgt cgacaagggc aaacagcggt ttcccggtgt cagcgtagtc gccgatgcgg
    25141 gtagtgagat tagtcaccca tccactcacc ggggcgcgaa tttccgtttg tgccagttgc
    25201 cattgtgcct gtttcagcgt cgcctgtgcg gcatcaacgc tggcctgcat cgctttaaca
    25261 ttgaggttag cggtatcgag ttcttcggca gaaataaaat tttgtgagag atgacgacga
    25321 cgattcgctt cgttattggc ttttgcgagg tcagattgcg ctttcgccag ctgagcctgt
    25381 gcgttcagct cggcgatctg aaagggcgtt ttatcgatcg tgagcagaag atcccccgcg
    25441 ttaaccagct ggttgtcttt tatattcagc tcaataatac gtcccgacac ctgtggggtg
    25501 atagaaacct gttctgcgcg tattttcccg tcgcgtgtcc acggagattg catgtaataa
    25561 ttccacagcc accatccggc aagaaccgca actaccgcta caatgattgt tgagaaatac
    25621 ttaattgttt taattgacat agttaccaca caatcagtat tgccagagca aggcaaacgc
    25681 aaatcgcaaa cagcgataaa tccattaaca agggatgcca gatgtcacca gcgtagatcc
    25741 agccacgaag caggcggtgt atgacaagcc agatgacgaa tccaaacgcg aaggctttga
    25801 aaaacggagg aaagtagacg gacgcaccga acaccagatc ttgtaagggc aatcctgttg
    25861 cattgagcat aaacttcaca aagtagattc ctttacgaca gaatatattg cgttagatta
    25921 ataaatattc tttaagtgcg aaaaatttac gcgcaatttc tgaaaaatat gtctgacggt
    25981 aaccaaatgc agcaatacat ttgttttagc aatacaattg ctgcacacta ttctaaaagc
    26041 cgcataatat cttagcaagc taattataag gagatgaaat tggaatcgcc actaggttct
    26101 gatctggcac ggttggtgcg catatggcgt gctctgatag accatcgcct gaaaccgctg
    26161 gagttaacac aaacccattg ggttacgtta cacaatatcc atcagttacc tccagaccag
    26221 tcgcaaattc aactggcaaa agcgattggc atcgagcagc catcactggt ccgtacgctg
    26281 gaccaactgg aagaaaaagg gttaatttcg cgtcaaactt gtgccagcga tcgtcgggct
    26341 aaacgtatta aactgacgga aaaggcagag ccactgatca gcgaaatgga agccgttatt
    26401 aacaaaaccc gcgcggaaat tttacatggc atctccgcag aggaactgga acaactgatt
    26461 aagctcatcg caaaacttga gcataatatc attgagttac aggccaaagg gtgaaatgaa
    26521 gggggcgtgt ggtcagtaac ctgaccacac gcaaacttat ttagcgcggg gaaacggtca
    26581 cctgactgcc attgctggcc agtacgacac gttggcctgg agagaaatga gtgttccctt
    26641 gtttctgtac caccatgatg gtgttgccat catctttacg aatttccagc tcgacaccct
    26701 gcgttttgtt cattgcactc tgtacgccct gaccggctac accacctgca acagcccctg
    26761 ctgcagtagc cagagaacgc ccggttccgc cgccaatagt atttcccagg aaaccaccaa
    26821 gtacagcacc accaattgcg ccgataacgt tggaatcatc accgccctga atctgtacct
    26881 gacgtacatt aacgatggtg ccatagctga cattctgtac ttgtttcgct tcagaagcgg
    26941 tataaacatc ccctgacagg gtgtcgttat taacacaacc gacaagagac agacctacca
    27001 ttgaaacaac caatacgcgt ttaatcattg aaaaatctcc tgttcaccat gaaacgccac
    27061 gcaagcatcc ctcatggtca aagtatatgg catatgtgaa tccatggtta catgttctac
    27121 caaatatgag caaatcataa tgaaagtagg atcaaccaat tataaacaaa atattcatga
    27181 tgattattga tttgaagata atgttttact accaacggta aaaattggtc attccgcatg
    27241 gtattcacga aaagtttatg tttgagtggt gcgcaaacgt cagattgaaa aaggatgaac
    27301 tatgaaatcg ggccgcttta ttggcgttat gtcaggcacc agccttgatg gtgttgatgt
    27361 tgtgttggcg acaattgatg aacaccgggt cgcacagctg gcaagtttga gctggccgat
    27421 cccggtgtct ctgaaacagg ctgtactgga tatttgccag ggccagcagc ttacactttc
    27481 gcagtttgga cagcttgata ctcaacttgg gcgacttttt gctgatgcgg tcaatgcctt
    27541 gcttaaggaa caaaacctgc aggcgagaga tatagttgcg atcggctgcc acggtcaaac
    27601 cgtctggcac gaaccgacgg gcgtggcacc acacacttta cagattggcg ataacaatca
    27661 aattgtggcg cgcaccggaa ttacggttgt cggtgatttt cgccgtcgcg atattgcctt
    27721 gggaggacaa ggtgcaccgc tggtacctgc gttccatcat gcgctgctgg ctcacccaac
    27781 cgagcgacga atggtgctca atattggcgg catcgccaat ctgtcactgc tcattcctgg
    27841 ccagccggtt gggggctacg ataccggtcc tggtaacatg ctgatggatg cctggatctg
    27901 gcgtcaggcc ggtaaacctt acgataaaga tgccgagtgg gcacgggcgg gtaaagttat
    27961 tctcccactg ctgcaaaata tgctctgcga cccatatttc tcgcaacccg caccgaaaag
    28021 caccggacgc gaatacttta actacggctg gctggagcgc catttgcgcc attttccggg
    28081 ggttgatccc cgtgatgtgc aggccacact ggcagaactc accgccgtga ccatttctga
    28141 acaagttttg ttgagcggtg gctgcgaacg attgatggta tgtggtggag gtagtcgtaa
    28201 tccgctactc atggcgcgtc tggcggcatt actgccaggc acagaagtca ccaccaccga
    28261 tgccgttggc attagtggcg atgacatgga agcattggcg ttcgcctggc ttgcctggcg
    28321 aacgctggcg ggattaccag gaaatctgcc ttccgtcact ggcgcaagcc aggagacggt
    28381 actgggggct attttccccg ctaacccgtg atatcagagt taactgaatt tttcttaccg
    28441 cgtctgccgt tacacttgga acgttaggga gggcgtattg ccctccagac caggaaagtc
    28501 ttcgggatat gtctatgaaa aaactgttac tgatctgttt gccggtgctt ctcaccggct
    28561 gtagcgcgtt caatcaactg gttgagcgca tgcaaaccga tacgctggaa taccagtgtg
    28621 atgaaaaacc gttgacggtc aaactgaata atccgcgcca ggaagtcagt tttgtttacg
    28681 ataatcaact actgcatctc aaacagggaa tttcagcgtc tggtgcacgt tacaccgacg
    28741 gaatctatgt tttctggtcg aaaggcgagg aagcgactgt ctataaacgc gaccgcatcg
    28801 tcttgaataa ctgtcagtta caaaatccgc agcgttgaga tttttccagg ggcggcgcac
    28861 aatagcgtca cccactgaca atccgtaaag aaaaccatgt ctgataacga cgaattgcag
    28921 caaatcgcgc atctgcgccg tgaatacacc aaaggcgggt tacgccgccg cgatctaccc
    28981 gccgatccat taaccctttt tgaacgttgg ctctctcagg cttgtgaagc caaactggct
    29041 gaccctaccg cgatggtggt cgctaccgtg gatgaacatg atcagcctta tcagcgcatc
    29101 gttttactca aacattacga cgaaaaaggc atggtgtttt acaccaacct cggcagccgt
    29161 aaagcacatc aaatcgaaaa taatccgcgc gttagcctgc tgttcccgtg gcataccctt
    29221 gaacgccagg tgatggtgat cggtaaagcg gaacgacttt cgactctcga agtgatgaaa
    29281 tattttcata gccgcccgcg tgatagccag attggggcat gggtttcgaa gcagtccagt
    29341 cgcatttctg cccgcggtat ccttgaaagt aaattcctgg agctgaagca gaagtttcaa
    29401 cagggcgaag tgccattgcc aagtttttgg ggcggttttc gcgtcagcct tgaacagatt
    29461 gagttctggc agggcggtga gcatcgcctg catgaccgct ttttgtacca gcgtgaaaat
    29521 gatgcgtgga agattgatcg tcttgcaccc tgaaaagatg caaaaatctt gctttaatcg
    29581 ctggtactcc tgattctggc actttattct atgtctcttt cgcatctggc gaaaagtcgt
    29641 gtaccggcaa aggtgcagtc gttatataca tggagatttt gatggcaagc agtaacttga
    29701 ttaaacaatt gcaagagcgg gggctggtag cccaggtgac ggacgaggaa gcgttagcag
    29761 agcgactggc gcaaggcccg atcgcgctct attgcggctt cgatcctacc gctgacagct
    29821 tgcatttggg gcatcttgtt ccattgttat gcctgaaacg cttccagcag gcgggccaca
    29881 agccggttgc gctggtaggc ggcgcgacgg gtctgattgg cgacccgagc ttcaaagctg
    29941 ccgagcgtaa gctgaacact gaagaaactg ttcaggagtg ggtggacaaa atccgcaagc
    30001 aggttgcccc gttcctcgat ttcgactgtg gagaaaactc tgctatcgcg gcgaacaact
    30061 atgactggtt cggcaatatg aatgtgctga ccttcctgcg cgatattggc aaacacttct
    30121 ccgttaacca gatgatcaac aaagaagcgg ttaagcagcg tctcaaccgt gaagatcagg
    30181 ggatttcgtt cactgagttt tcctacaacc tgttgcaggg ttatgacttc gcctgtctga
    30241 acaaacagta cggtgtggtg ctgcaaatcg gtggttctga ccagtggggt aacatcactt
    30301 ctggtatcga cctgacccgt cgtctgcatc agaatcaggt gtttggcttg accgttccgt
    30361 tgatcaccaa agcagacggc accaaatttg gtaaaactga aggcggcgca gtctggttgg
    30421 atccgaagaa aaccagcccg tacaaattct accagttctg gatcaacact gcggatgccg
    30481 acgtttaccg cttcctgaaa ttcttcacct tcatgagcat tgaagagatc aacgccctgg
    30541 aagaagaaga taaaaacagc ggtaaagcac cgcgcgccca gtatgtgctt gcagaacagg
    30601 tgacacgtct ggtgcatggt gaagaaggtt tgcaggcggc aaaacgtatt accgaatgcc
    30661 tgttcagcgg ttccttgagt gcgctgagtg aagcggactt cgaacagctg gcgcaggacg
    30721 gcgtaccgat ggttgagatg gaaaagggcg cagacctgat gcaggcgctg gtcgattctg
    30781 aactgcaacc ttcccgtggt caggcgcgta aaactatcgc ctccaatgcc atcaccatta
    30841 acggtgaaaa acagtccgat cctgaatatt tctttaaaga agaagatcgt ctgtttggtc
    30901 gttttacctt gctgcgtcgc ggcaaaaaga attactgtct gatttgctgg aaataatgca
    30961 ttaagtggaa agggggagtg agaaatcact ccccctggtt tttatacagg gaacatgatg
    31021 aaaaatattc tcgctatcca gtctcacgtt gtttatggtc atgcgggtaa cagtgcggca
    31081 gagtttccga tgcgccgcct gggcgcgaac gtctggccgc tgaacaccgt tcaattttct
    31141 aatcacaccc aatacggcaa atggactggc tgtgtgatgc cacccagcca tttaaccgaa
    31201 attgtgcaag gcattgccgc cattgataaa ttacacacct gtgatgccgt attaagtggc
    31261 tatctgggat cggcggagca gggtgaacat atcctcggta tcgttcgtca ggtgaaagca
    31321 gcgaatccac aggcgaaata tttttgcgat ccggtaatgg gtcatccgga aaaaggctgt
    31381 atcgttgcac cgggtgtcgc agagtttcat gtgcggcacg gtttgcctgc cagcgatatc
    31441 attgcgccga atctggttga gctggaaata ctctgtgagc atccggtaaa taacgtcgaa
    31501 gaagcggttc tggcagcgcg cgaactcatt gcgcaaggac cacaaattgt gttggttaaa
    31561 cacctggcgc gagctggcta cagccgtgac cgttttgaaa tgctgctagt caccgccgat
    31621 gaagcctggc atatcagccg tccgctggtg gattttggta tgcgccagcc ggtaggtgtt
    31681 ggtgatgtga cgagcggctt actgctggtg aaactgcttc agggggcaac gctgcaggag
    31741 gccctggaac atgtgaccgc tgcagtatac gaaatcatgg tgaccacaaa agcaatgcag
    31801 gaatatgagc tgcaagtggt tgctgctcag gatcgtattg ccaatccaga acattatttc
    31861 agcgcaacaa agctctgaaa tatctcattt cggcccacaa cgacctgtgg gccgatggct
    31921 attactttaa gccttccgct gacagcgcgt cttgtacttc cggacgttca gccatacgtt
    31981 gcataaatgc cgcaatgtgc tctaaccctt ccagattcag tttcaccgcg tatgcccagc
    32041 gcagaaccgt aaacagatag gcatcagcaa ttgtaaatcg ttgcccgcag atccaatgct
    32101 catccttcag tgcctcgttc acatattgca gcttcttctc cagctgcgcg cgaactgtcg
    32161 atttgtactc ttccggtgta tcagggcgaa acagaggtgt gaaaccttta tgcagctcgg
    32221 tggcgatgta attcagccat tcgatggttt tatagcggga aatactgttt accggtgcca
    32281 gcaactggcg gtcggggacg ctgtcggcaa gatactgcat aatcgctacg ccttccgtca
    32341 gcaaagtacc atcgtccagc agcaatgcag gtacttgtcc cttagggtta acggcaaagt
    32401 aattgtcacc gttttcgaga cgttttttca ttaaatccac actgacgagc gtaaaatcct
    32461 ttccgctctc acgcagggtg atatgggaag cgagagagca ggcgcccggt ttgtagaaca
    32521 atttcatcgg taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt gctgaacaaa
    32581 aaaaagccgc taacgaaaag ttagcggctt gtaaaagagt ttccctgaat tttacgctac
    32641 ggctgctttc gccgctttgt ctgcagcgtc atcctgcgtc atgcggtgca gtttcggcgc
    32701 ggtaaacagc atcagtactg caataacggc agtagcgaca ccaatctgca agaatacgcg
    32761 accatagact tccagtgaca tcagcggatc ggtaacgtta tccggcacag ccatcatacc
    32821 tgcaacataa ccaccaatca ggtttgcacc ggcagtggtc aggaaccagc tacccataat
    32881 gaagcccatc agacgctgcg gaacgagttg tgcaaccatt gccagaccca ggccagagat
    32941 catcagttcc ccgatactct gcaggccata gctcgcgacc agccagctta cagacacgat
    33001 accagcgtca gacgcgaatt tcgcacccag cggcagaatc aggaacgcac cagaacacat
    33061 caccatgccg atagcaaact tggttggcat tggcagggta tcgcccatct tgttatagat
    33121 agcggccaga atcgggctac caatgatgat ccagaacggg ttcagtgcct gatactgctc
    33181 aggttctacg gccaggccca gaatggtgtg ttcaacgtta cgaatcgcaa agaagttcag
    33241 tgacgttggc atctggctat aaagcacgaa gaagataatg gcttcgagca tcaggatgaa
    33301 ggcaacgatc attttacgac gcgcagcacc tttcatcgcg aaggcttctt taccgaagat
    33361 aaccacgata ccgaaggcaa caacgcccag cgccatacgc gcaacttcct gattgtgcag
    33421 cagccaggtg gcgatagcga tcagtgccac aacaccaata atggtcagca gcaggttacg
    33481 gtagttgata ggctcgaagt ctggttttga accgtactgt ttaacccaac gttggcagaa
    33541 ggcgaagtta acgatagtga tcagcaggcc tacaacgctc aacgcaaacg caacactcca
    33601 gccgtatttc gcggccagcc acggcgtagc aatcatagag aagaaagagc cgatgttgac
    33661 ggacatgtag tacatggtga atgcaccgtc cagacgcggg tcgtttttct catagcatgt
    33721 agaaagcaga gaagacgggt tagctttaaa caggccgtta ccgaccgcaa tagccgccat
    33781 acccatataa acgataccgg cgtcgtgacc agaccaggca accagagcat aaccaatcgc
    33841 cagcacaata gcgccgagca taattacgcg tttagtaccc agtaccttgt cacctaacca
    33901 gccgccgata gcgaccagac cataaaccag ggcactaaag gaagagaaaa gggtgattga
    33961 atccgcttca gacataccca gttgtttaac caggtaaaca gccataattc cttgtaggcc
    34021 gtaataacca aaacgttccc ataactcaat cgagaagatg agatagaacg ccttcggttg
    34081 tttaaaagcg ttcaaactga cgctttcagt tggtttttgg tttgcagtgg acacttttac
    34141 ctcttttttt acatcccata ttaacggggg tgctttctcg cgttatgtct aaaaaacata
    34201 cgcgcaattg ttatagtggg aggggaaacg gcaggtaatg ttcactatct tgcactttca
    34261 gacaagtcct ttgtaataat ctgttacata taactgaggc ggggaattca accattccgg
    34321 gaaatgttat ccagcgttaa atttttcaaa aaacgtaacg gcagattttt tcactaccct
    34381 gagagggtga tgatggctta atggcgatat gttctactat ttggcgtttt tcctagtgag
    34441 atagtccagt ttctgaaaaa tatccagtgt aatgttttgt aggtcaatat ttgttgcgtg
    34501 tgtaaattta aatgatttta acctgattct tgcaatgttt ttacattgcc acagcatcgt
    34561 gatcttgatc acgaatgaat agaaaatttc gctattaaaa aaacaattaa cctgcatttt
    34621 tggttatctg caaacggatt acctgatgat aacgctaagc aatggcatca ttgacgttaa
    34681 aaagatgttt taaaagagga taaagaaagg ttatgaatgg ggtaatcggt gttaccccta
    34741 atctttagat gtcaactttc tctttgtatt cacaaagatc ttcaataata caagagccac
    34801 agcgaggctt gcgggcaatg caggtataac gcccgtgcag gatcaaccaa tggtggcagt
    34861 cgactttaaa ctctgccgga accactttca gtagcttttc ttctacctgt tcgacgtttt
    34921 tccccggcgc aaattgagta cgattacaaa cgcggaaaat gtgcgtgtcg acagcaatag
    34981 tcggccagcc gaatgcagtg tttaatacga cgttggctgt tttacgacct acaccaggca
    35041 gggcttcaag cgcagcacga tcttccggaa cctcgccatt atgttgttcc agtaatatac
    35101 ggcaggtttt gatgatattt tctgctttgc tgttataaag gccaatcgtt ttgatatagg
    35161 ttttcacccc ttcaacgccc agttcaagca tcgctgcagg cgtattagcc accgggtaga
    35221 gtttcgccgt cgccttatta acactgacat cggtcgcctg agcggaaagc agtacggcaa
    35281 tcagcaattc aaaaggcgaa ctgaaattaa gctcggtggt gggatgagga ttgttctcac
    35341 gcaggcgagt gaggatctcc aggcgttttg ctttgttcat cagacattcc ctgtttcacc
    35401 gtttggcaat gcacgttcgg caactgcttc agtacggcgc tttttcattt tttcatcaat
    35461 caggtatttt cctgccagca tcagtcccag gccaataaat gcacctggtg gcagcatcgc
    35521 cagcaggaaa ggggagtcgg tgtggaaaat ctccacgcgt aatactttcg cccagctacc
    35581 taacagcgca tctgcaccgt caaacaacgt gccattgccg ataatttcgc gtagtgaacc
    35641 cagcacgaac atggcgcagg ttgcgcccat accaattgaa aagccgtcca gtgccgaaag
    35701 cgccggacct tttttggcgg cgaaggcttc agcgcggccc acaacgatac agttagtgac
    35761 aatcagcgga ataaaaatcc ctaatgattg atacaggcca aaggcgtagg cgttgatcag
    35821 catctgtaca gcgctgacca ccgaggcgat gatcatcacg taaatgggaa tgcggatctc
    35881 ggcgggcgtc cagtgacgca gcgtcgaaat ggtcaggttg gttagcgtca gtaccagcgt
    35941 agtcgcaagt cctaaaccca gagcgttagt ggcagtggac gtgaccgcca acagcggaca
    36001 aaggccgagc aactggacca gcgcagagtt gtttttccac aacccctgaa caataacgtc
    36061 tttaatttcg ctcacgtttt attctccaca ggcaggaagt tgagaaagtt gtgccggtaa
    36121 cgtctgagcg tacaatccgg cgcgttttac cgcattaacc accgcgcggg gagtaatcgt
    36181 cgcgccggtg aactggtcga aatcaccacc atctttcttc accgcccagt tcgcatcatc
    36241 cgcaccactg atttttttac ccgcaaaatg ggtgatccag tcagaaaggc gcagttcgat
    36301 tttatcgcca agccctggcg tttcgtggtg ctcagtcacg cgcgtgccaa gtaccgtgcc
    36361 gttaaaatcg gctcccacca gcagctgaat cgcaccggaa tagccatctg gcgcggttgc
    36421 ttccagaacg gcggctacag gtttgtcatc ctgtttggcg atgtaaaccc gatgctcacc
    36481 tttacctaac tctggcgcag tgaccagata gcaactctgt gccagcgcat tgttatagcg
    36541 ttcggctggc agcacctgat caaataacgc cttttgttgc agactggcct gttcagcaat
    36601 tgtcgttttg gtcatctggt tgatggccgc agttaaccct gtagaacccg ctgcaaatag
    36661 tgccagtgta atgccgtgtt ttcggatagt tttcagcatg gtttaccctt tgcgatggcc
    36721 gtagacgcgt ggacgcgtgt agtaatcgat cagaggaacc gtgatgttcg ccagcaggac
    36781 ggcaaaagcc acgccgtcag gatagccgcc gaaactgcgg atcaaccaga ctaataaacc
    36841 cgccagcgcg ccgaaaataa gacgaccacg attggtcgta gaagcagtaa ccgggtcagt
    36901 caaaataaag aatgcgccga gcatggtcgc accagacagc agatgaattt gtggtgctgc
    36961 cagagtatct ggtgagaaca accagcccaa cgttgcgcat aacgccagcg ttactaagaa
    37021 gctgagggga atatgccagc gaatcgcttt ctgccatagc aaccacacgc cgccagccag
    37081 ccaggcgaga tttacccatt gccaaccagc gcccgccaga ataccgctgt agatcggata
    37141 ttgcataatc tcttcaaccg agtgaccggc acggacagag gttttaaatg tatccagcgg
    37201 tgtcgcctga ctaatgccat caatacctaa gcgtagcgtg ttcatatcac caccactggc
    37261 ggtatgacca ctgaaaataa cctgtatggt gtcgataaaa ccagggatgt tgaccgcaat
    37321 ttcatgtggc ggtaaccaac tggtcatctg caccgggaag gagatcagta agaccacata
    37381 accaatcatt gccggattaa acgggttttg ccccagaccg ccatacaact gtttagcgat
    37441 gatcaccgca aacaccgtac ccagcacgac catccaccat ggcgcgaggg ggggaatact
    37501 taccgccagc aataagcctg tcagcaatgc tgagttatct ttcaacgttg cagctaccga
    37561 ctgcttgcgt agtttgagga cgagagcttc ggccagaaga gcactgaccg acgccaacag
    37621 gatctgaacg agagtacccc aaccaaaaaa ccacagttgt gctgcgattc ctggcacggc
    37681 tgcgagcaat accaacagca taatgcgcga tgtctggcgc tggttatggg tataagggga
    37741 gctagctatt ctgaatacca tttagtcctc gtttacaacc ttttgctggg cggctttttt
    37801 ggcctgaacg cgggcaatag ccgcagcaac tgccgctttg cgcggatcaa tctgttcttc
    37861 tggctctgca ttagcctgtt gctgttccag cttgcgcgct ttggcgcgtg caatagccgc
    37921 ttcgatggca gctttgcgcg gatcaatctg ttcttctggc tccgcattag cctgttgctg
    37981 ttccagcttg cgtgctttgg cacgggcaat agcagcttcg acggcggctt tgcgcggatc
    38041 aatctgttct tctggctccg cattagcctg ttgatgttcc agcttacgtg ctttggcgcg
    38101 ggcgatagcg gcttcgacgg cgactttgcg cggatcgacc tgttgttctg gctccgcatt
    38161 ggcctgttgc tgttccagct tgcgtgcttt ggcacgggcg atagcggctt cgacggcggc
    38221 tttgcgcgga tcgacctgtt gttctggctc cgcattggct tgttgctgtt ccagcttgcg
    38281 tgctttggcg cgggcaatag ccgcttcaac tgcagtttta cgtggatcag caacggttgc
    38341 agcgtcgtta gtttgctgca gttctgcctg tttcgctctg gcttgcgctt tacgggcttc
    38401 ccgtgctgca ataattgcac tgttatccgg gcgttcgccc gctttaatca caataggctg
    38461 tgtagcctgg gcctgtttct ctttcacccg cgccagggcg gcagcaatcg catctttatc
    38521 tttggctgca ggttgaacgg ctgcgctctt atgtcgttca aggcgagccg ctttttcgcg
    38581 ctccagacga gcctggcgcg cttcgaaacg cgctttggct tctgcggcgc gcttttcttc
    38641 ctgacgaata gccgcaattt cagctttttc ctgacggaaa tattgcacca gggggatatt
    38701 gctcgggcaa acccacgcgc aagctccaca ttcaatgcag tcggcaatgt tatgcgtggt
    38761 ggctttatcg tgttgctgac ctttgctgaa ccagtacagc tgttgcggca aaagatccgc
    38821 agggcaggcg tcagcacagg cgctacaacg gatgcaactt tgttcttcct gtggttcgcc
    38881 aagttcattg gcagagggag ccaacagaca gttggtaatc tttacgaccg ggacatccag
    38941 ccacggcaag gtaaagccca ttagcgggcc gcccataatc accatttggt cggcagaggg
    39001 gcagaatccg gcgtcattca ataaatgacg cactggcgtc cccaaccgtg cccagacgtt
    39061 gcccgggcga gcgattgctt cgccagtcag ggtcacgaca cgctcggtaa tcggctcgcc
    39121 atcaataacg gcacgtttca ctgcataagc ggtgccgacg ttttgcatta atacgccgat
    39181 atcggatgaa cgcccgccat gtggaacctg cttcccggtc agaatgtagg ttaattgttt
    39241 agcaccgcca gaaggatatt tggtgggaat cacccgcaga gaaatatcgt gagagtctgc
    39301 cagcaccgcg cgcagcatgg aaatcgcctg cggtttgtta tcttcaatac cgataagaat
    39361 ttcgcgtggc tgcagaatat gcgcaaggat gcggataccc tctacgacct gagccgcaca
    39421 atcctgcatc aaacggtcat cggcggtaat gtacggctcg cactcagcag cgttgataat
    39481 caacgtttca atcttatctc cgccgccctg caatttaacg cctgtcggaa atcccgcacc
    39541 acccagcccg gcaacaccaa actgatgaat gcgctcgatt aactcttcgc gactgcgaga
    39601 gcgataatcg gcccagccgt cgcgcgggat ccagcagtct tcgccatcgg catcaataat
    39661 cacacttaat tcagctaaag ctgaaggatg agccgtagag tggggcgcaa tagccgtaac
    39721 ggtacccgag gtgggcgcgt gaacaggcag cattttgccg cgaccacggg taagcggctg
    39781 gccgcgcaat acgttatcgc cgacgctaac gcacaactca ccttcagcgc caatatgttg
    39841 tttcagtgga atgacaaaac gctgcgccag tggtacctgg cgcaggggcg taccgttgga
    39901 ctgggttttc atctccggtg gatggatgcc gccgttgaaa tcccagattt tattttttct
    39961 gaatgcagag aataacttaa gcatggtgtt ccacgggaat gatacgcacg ggaatggtgt
    40021 tcagatccca tttccaggag tcaggagttt ctgcgaccgg ttgcaacgag atgcagtgtg
    40081 tcgggcacgg atcaacacat aagttgcagc ccgtacagag atcactcatt accgtatgca
    40141 tggcgcgggt agcgccaacg atggcgtcta ccggacacgc ctgaatacat ttagtgcagc
    40201 caatacagtt attttcatca ataaccgcca ccatccgcgc tggcgttagc tcttgcgctt
    40261 cgccatccag cggctgcggc tcgacattaa gcaactcggc aatttttagc atcacagctt
    40321 cgccacctgg ggcgcaacgg ttgatttttt caccgttaca gctgatggct tccgcgtagg
    40381 ggcgacagcc gggataaccg cactgaccac actggctctg cggtaagatt tcgtcaattt
    40441 tctcaacgac cggatcgtct tccaccgcaa aacggcggga ggcataaccc agaatggcgc
    40501 caaacgccag gcccagcagg ctcacggcgg caacggcaat ccagatagca ttcattacaa
    40561 cttcaccaaa ccactaaagc ccataaaggc cagagacata agacctgcgg taattaacgc
    40621 aatggcatta ccgcgaaaag gtgccgggac atcagccaca gcaaggcgtt cgcggatggc
    40681 ggcgaagagc accatcacca gcgagaaacc gacagcggcg gaaaaaccgt acagcgctga
    40741 ctgcaagaaa ttgtgcccga gattgatatt cagcaacgcc acgccgagca cggcacagtt
    40801 ggtggtgata agcggcaaaa aaatccccag cagacggtaa agtaccgggc tggttttgcg
    40861 caccaccatt tcggtgaact gcacaaccac agcaatcacc agaataaacg ccagggtgcg
    40921 caggtaaatc agattaagtg ggatcaaaat ccacgtatcg ataagccagg cgcaaataga
    40981 cgccagcgtc atcacaaacg ttgttgccag ccccatgccc atcgcggttt ccagcttttt
    41041 ggaaaccccc ataaacggac agagaccgag aaacttgacc agtacaaagt tattgaccag
    41101 tacagttccg acaaacagta acaggtagtc agtcattttt cggcctgaaa taaaaaagcc
    41161 gcctattatc cgttaatcca gagcaggcga caacaggtta actgtaaggt tattacggat
    41221 tcacaaacgt cgctttcacc cgcgacgagc gtttgaaata ggggacgatc aatgcggttg
    41281 ccaacaaagt aaacagcaac tggcgaacgg ctatgccatc ttctacgggt gaaaaagcga
    41341 acgcttttac tgccagcaat acggagataa gcagccagat aatatagtgt ttaggaacac
    41401 aacgccgacg tttaaagaaa gcgatggtca accacagtgt gtaataccac atggcgatag
    41461 cggtaataaa agaaacgccc cataagattt gtgtcgtcag tgcttgtccg ccgagcgttt
    41521 gaaatgtttg cggagaagat aacgcagcgg tatacagcaa caacgccagc gtcgtactca
    41581 acagagcgac taaaagccag gccaacgggc caagcaacca gcctccaata cgttgtggcg
    41641 ttgtggtggt cataggttct ccagaatagc gacgcaaaca gacaaatcta attttagggg
    41701 cgttattata aggcttttat aaccgatcgc tactttcttt actggacata gcgccagacg
    41761 gacttcggta cctggccgag gtcaaacaaa cgcccgccag aaaccagctc tgcgcgacgg
    41821 tgatcggcag cacgatacat attgatcagt tcctgatcgt ccgtcagggt gtaattaaga
    41881 tgatcgtaga gtttttccag actttcgagt gaactgattt tgcgaaattt taataagtag
    41941 tcctgaacag tcataaaagg tccataaaat aattaatacc aaaggggtag gtattattag
    42001 tgatgataaa atttcaccaa aagtgaacgc ttgtgttatt acaataataa gaatgtgctg
    42061 atggcaaata ttcagggatg aaaaactgct ctggaaagag taacgcgctt tactatccct
    42121 gacaatcatc aacaacatcg aacaagataa taaattcctg gtttaatatc cgacaagtga
    42181 aaacatgcac ccggacgggc agcatgtcgc tcaacaagtg cagagcttac ttgtgttgta
    42241 ctgtagtact ctgttcaggt ggctgatagt tgtcaatgtg actcgccacg ccaagaagaa
    42301 tgactgaaac gacaagaacg atccaacctg ttaattcaat aagacgattc attacagccc
    42361 acatctctgt tgattgatcc attaacttca gggggtaaat gttacttagc aataatagct
    42421 ccagcaagat ttttactgag gtttttgcga tatttagctt ttgtcgttgg aaaaatcgct
    42481 atttttttga cttaagttaa acaacatccc ttattgctgg caggttatta aactgttgag
    42541 cgaggataaa ggatagtgtc aaatagccat catacttcaa tgagaggcaa tgacatgagc
    42601 gacaacatcc gtgttgggtt gattgggtat ggttatgcga gcaaaacctt ccatgcgccc
    42661 ctgattgcgg gcacgcccgg gctggagctg gcggtaatct ccagcagcga tgaaacaaaa
    42721 gtaaaagctg actggccaac ggttacggtt gtctctgagc cgaagcatct gtttaacgat
    42781 cccaacatag acctgattgt cattcctaca cccaacgata cccattttcc gttagccaaa
    42841 gcggcgcttg aggcgggtaa acatgtggtc gttgataaac cctttaccgt gacactgtca
    42901 caagcgcgag agctggatgc gctggcaaaa agcctggggc gagtgctgtc tgtattccat
    42961 aaccgtcgct gggatagcga tttcctgacg ctaaaaggtt tgctcgcgga aggcgtgctg
    43021 ggtgaagttg cttactttga gtctcatttt gaccgcttcc gtccgcaggt gcgcgatcgt
    43081 tggcgtgaac agggcggccc aggcagcggt atctggtacg atttagcacc gcaccttctt
    43141 gatcaggcca ttacgctatt tggtttaccg gttagcatga cggtagattt ggcacagtta
    43201 cggcccggag cgcagtcgac cgattatttc cacgctatct tgtcctatcc gcagcggcga
    43261 gtgattttac acggcaccat gctggcagca gccgagtcag cacggtatat cgtgcatgga
    43321 tcccgaggca gttatgtgaa atatggcctc gatccacagg aagaacgtct gaaaaatggc
    43381 gagcgtctgc cgcaggaaga ctggggctac gatatgcgcg atggcgtact tacccgcgtg
    43441 gaaggtgagg aacgtgtcga agaaacgctg ttgacagtgc cggggaatta tccggcttac
    43501 tatgcggcta ttcgtgatgc gttaaatggc gatggtgaaa atccggttcc ggcaagtcag
    43561 gcaatccagg taatggagtt gattgagctg ggcatcgaat ccgccaaaca tcgcgcgact
    43621 ttgtgccttg cgtgaaaagg ggatggtgca attagcacca tccatttttg ttacttcgcg
    43681 gcgacttttt ctcgcagtgc gcgtttttcc tctgcgctga ggaaagccat ttccagacca
    43741 ttaatctgtg cctggcggat ttgctcgcgg gataacccag cagctggcgc ggcaacggta
    43801 tattcgtgaa tgatatccac tccctgcacg ccgggatcgt cagtgttaat gctggcacga
    43861 atgccatgct caaggaacgt tttcagcgga tgtgcagcca gatccgccac ggtgctggtc
    43921 tgaatattgg aggtcagaca ggattcaata ccaatttgtt gctcggcgag aaaatccatc
    43981 agcgcccgat cttcaatggc ttttacgcca tgtccaatac gctccgcccc cagttcacga
    44041 atcgcctgcc agatgctttc cggtccggca gcttcgcctg catggacggt aatatgccag
    44101 cccgcatcac gcgcgcggtt gaaatgagaa aggaacagac ttcccgggaa accaagttca
    44161 tcaccggcta aatcaagtgc ggtaatctgg tcacggtggg ctaaaaaggc ctccagctct
    44221 tgctgacagg cggcttcgcc gaaggtccgg ctcataatac cgataagctt cgcctgcaca
    44281 ccaaaggtgc ggcaaccttc acgtacgcca tcgatcaccg cttcgacaac acccgctaca
    44341 ggcagctgat gtgccattgc catgtagcct ggtgaaaaac gcagctcgac atagtgcagg
    44401 ccgttacggg ctgcatcttc aatgttttca aatgccacgc ggcggcaggc atcaagagag
    44461 gcgagaactt taacgcccca gtcaagttta gtcagaaagc tcaccagatc gggttcgttg
    44521 gcaatgacct gaacgtgggg aatcagtgtt tccagggatt gtgcaggaag cgagatatta
    44581 tactggcggc caagttcaag aatggtctgg ggacgaatgt tgccatcaag gtggcgatgg
    44641 atatcagtta atggcagggt ggtatcaatc atggtcgcac tctttttctg gatataaaag
    44701 tgcgcaccat tataaaaaca aagcagggta aaaatccacg attgcgcaac gattttttac
    44761 gttgcgcaat tggggttaac caacagcgcg gatggcgtta attaatccag ccacaccttt
    44821 ttccagtttc gaacgtgggc agccggcatt gagtcggata aaaccacgac cttcttcacc
    44881 gtaggtatat cccggcatga tcgcgatctt ttcttgctcg ataagcgctt tttgcaacgc
    44941 gttgtcgtca atattcaacg gacgtagatc aagccaggcc agataagtgg attgcgggat
    45001 ctgccagttg agttcaggaa acgcagcgtt cattttatct gcgatatacg tcaggttatc
    45061 tttcagatag acgcgtaagg catccagcca tggcgcgcct tgctgatagg cggcgatatg
    45121 agcagttaac gccagtaccg aaggggaaga aagcccatca cgtcctttca gtgccgataa
    45181 ataggcatcg cggccactgc tattttctat aatcccgtaa gcaccggtca gggcgggaat
    45241 attgaaactt tttgatcccg acgttagcaa cgcccagtct ccgcgcgcca cattactcca
    45301 gggaatatgc ggctgctcgc cccaaaccat atccatatgg atttcatcgg aaataacccg
    45361 cacaccatga cgctcgcaca ggtcagccat gatctccagc tcatcgcacg tccacacttt
    45421 cccggtagga ttctgtgggc tacacaggag cataatttta cattctggtt tcgctagcac
    45481 ggctttcagc ttgcccatat cgcaaaacca gccgtcagcc tgcttctcta aagcaacggg
    45541 cattactgtg cgctggttac cttcaatggc cttgtaaaat gcgtcatagg cgggtgtgtg
    45601 gatcaccacg ccttcacctg tttcagacca ctgacgaatc agttctgaaa ccatatagat
    45661 aacagaaggg ccatacacca ccgactgagg atcgatggcg gtgtaatgct gggtggaaaa
    45721 ccagtgggca atagccgcga gaaactcatc gtttttccag cggctgtagc caaacacgcc
    45781 gtgtatcagg cgctgattca gcgcctcgat aatgcagggg gcagtggcaa aatccatgtc
    45841 tgaaatcgtg aacggtaaca ggtcagcagt gccgaaacgg tcagcgacat aatcccactg
    45901 cgtacaccat gtgccatgac gatccacgac ctttgaaaaa tcgaacatat cttatcctta
    45961 tgcctggaca gtacgcatca gaccgaccat ttcatctttt actgactgaa cttgtggccc
    46021 gataacaacc tgcaggttat gttgattaag ttgtactacg ccaattgccc gattgtcctt
    46081 cagtgcctgc acattaacaa gcgacatatc ttttacagac aaacgcagac gcgtaatgca
    46141 gttatcgaga ctgacaatat tgtcggcacc gcctaaggct tcgaggattg ccggaacgtt
    46201 gtaacctgat ttacccggcg caccggcaac ggctttttcg attgaactgg caacttcact
    46261 atcgcgcccc ggggttttga gattgaagcg ggtgatagcg aaacggaaga tgatgtagta
    46321 aacgacaaac cagattgccg ccactactgg caccatgtac cacttggttg acagaccatg
    46381 caaaataccg aacaccacaa agtcgatgat attgccgtcg gtattaccga tggtgacgcc
    46441 gagcacagac atgacagtga agccgaggcc ggttaacagc gcgtggatga catacagaac
    46501 tggcgctacg aacaggaaca ggaattccag cggttcggtc gtgccgccaa cgacacaggc
    46561 gatcaggcca gaaatcagca gacctttaat tttatggcga ttttccgggc gcgcacagtg
    46621 atacatagct aacgctgcac ctggcagacc gccgagaaac gcaggcattt taccttgcga
    46681 aaggaaacgc gtggcgcttt cagaaaaacc gtgagtggtt gggcaactca attgcgcctg
    46741 gaagatggtc agtgcaccgc tgacggtttg accgcagact tcctgcgtgc cgcctgcgtc
    46801 ggtaaagcga attaatgcca ccagaatgtg atgcagacca aacggcaaca gcagacgttc
    46861 accggtacca aacagcatcg gtccgaaatc tcccgcgctg ttgatcatat ggcctaagcc
    46921 gctaataccc atggcgaaaa tcggccagac taatggaatc accaggccga caagacccat
    46981 cactagcgag gagatgattg gtacgaagcg cgtaccgccg aagaatgcca gcgcatccgg
    47041 caggcggata ttatggaatc gctcatgcag catccagacg ataataccgg cgatcaccgc
    47101 accgaggatc ccggtgtcga tcgactggat cccaagaatg ctctggatgt tattggcttt
    47161 cagaaccgcg gcgtccgtgg ttggcagaat gcctttattg gtcaaccaga agtttaccgc
    47221 gaggttcatt accgcataac cgacgaagcc agcgaatgcc gctacgcctt tgttttcgcg
    47281 tgccagaccc agcgggatgg cgatacagaa catgactggc aggaaactaa aagcaaacga
    47341 gccaatctta ctcatccagg taaagatagc ctgcaacacg gggttgccca ggaccgggat
    47401 cagggtgatg acatcatgac tgctaagaga actaccaatg ccgagcataa tgccgcagaa
    47461 cgacaataat gccacgggta acatgaaggt tttgcctaac tgctggaaga actcccacag
    47521 cgtgactttc ggtgctgttt tcgccgtcat aaaacgactc ctcgtagaga acaatctgga
    47581 ttcagtaaat tgcgcgagaa gataaaacgt tttatcaaat tttagtgagg cataaatcac
    47641 attacgcaac gataatagcg ggtataagat aaataaaagg taaaacgttt tatctgtcac
    47701 ataatcaggg agtaggtcat ctgcatggct accgccaaaa aaataaccat tcatgatgtt
    47761 gcgctggctg cgggcgtgtc ggtaagcacc gtttcgctgg tgctaagtgg caaagggcga
    47821 atctctaccg ccacaggaga acgcgttaac gccgccattg aagagctggg atttgtgcgc
    47881 aatcgccagg cgtcggcgct gcgcggcggg caaagcggcg tcattggttt gatcgtccgt
    47941 gatttatctg cgccgtttta cgccgaattg acggccggat tgacggaagc tctggaagcg
    48001 cagggacgga tggttttttt gcttcacggc ggtaaagacg gcgagcagct ggcacagcgg
    48061 ttttcactat tactgaatca gggtgtcgat ggtgtggtca ttgccggggc tgcaggaagc
    48121 agcgatgacc tgcgacggat agcagaagaa aaagctatcc cggtgatttt cgcttcccgt
    48181 gccagttatc ttgatgatgt tgatacggtt cgcccggaca acatgcaggc tgcacagttg
    48241 ttgacggagc atctcattcg caatgggcat cagcgtatcg cctggctggg agggcaaagt
    48301 tcctcattaa cccgggcaga acgggtgggg ggctattgtg cgactctact aaaatttggc
    48361 ctgccgtttc acagcgattg ggtgctggag tgcacttcca gccagaagca agccgcggaa
    48421 gctatcacgg cgcttttacg tcataacccg accatcagcg ccgtggtttg ctataacgaa
    48481 actattgcga tgggggcatg gtttggtttg ctgaaagcag gaaggcaaag cggggaaagc
    48541 ggagtcgatc gttactttga gcaacaggtt tcgctggcgg catttaccga tgcaacgcca
    48601 accacacttg atgatatacc cgttacctgg gccagcacgc cagcgcggga acttggtacc
    48661 acacttgcgg atcgcatgat gcaaaaaatc acccatgaag agacgcattc acgcaatctt
    48721 attattcccg cccggctcat tgcggcgaaa taatcctctc tttatctgct atacctggta
    48781 gtgtcctttc ctcaaggtta atgataaaaa atgtggcaca caggcggtgc cttttatttt
    48841 tactgaagtt cacaggaggt ttatgtgttt aattctgaca acctgagact cgacggaaaa
    48901 tgcgccatca ttacaggtgc gggtgcaggt attggtaaag aaatcgccat tacattcgcg
    48961 acagctggcg catctgtggt ggtcagtgat attaacgccg atgcagctaa tcatgttgta
    49021 gatgaaattc aacaactggg tggtaaggca tttgcctgcc gctgcgatat tacttccgaa
    49081 caggaactct ctgcactggc agactttgcc gtcagtaagt tgggtaaagt tgatatcctg
    49141 gttaacaacg ccggtggcgg tgggcctaaa ccgtttgata tgccaatggc agattttcgt
    49201 cgcgcttatg aactgaatgt attttctttt ttccatctgt cacaacttgt tgcgccagaa
    49261 atggaaaaaa atggcggtgg cgttattctg accattactt ctatggcggc agaaaataaa
    49321 aatataaata tgacctccta tgcatcatct aaagctgcgg ccagtcatct ggtcagaaat
    49381 atggcgtttg acctgggtga aaaaaatatt cgggtgaatg gcattgcacc gggggcaata
    49441 ttaaccgatg ccctgaaatc cgttattaca ccagaaattg aacagaaaat gttgcaacac
    49501 acaccaatca gacgtctggg ccaaccgcaa gatattgcta acgcggcgct gttcctttgc
    49561 tcgcctgcag ccagctgggt aagcggacaa attctcaccg tctccggtgg tggggtacag
    49621 gaactcaatt aatacactaa cgaaccggta aacagccgta tgtgttgttt accgggatag
    49681 cgtgtgcaac tcatcaacgt ctctgctaaa caactggcag ccaaatcacg gctattggtt
    49741 aaccaatatc tggataaaaa gcttacgaat tgagtgtgcc ttcgcactat tcaacagcaa
    49801 tgataggcgc tcacctgaca acgcggtaaa ctagttattc atgctaacta taatggttta
    49861 atgatggata acatgcagac tgaagcacaa ccgacacgga cccggatcct caatgctgcc
    49921 agagagattt tttcagaaaa tggatttcac agtgcctcga tgaaagccat ctgtaaatcc
    49981 tgcgccatta gtcccgggac gctctatcac catttcatct ccaaagaagc cttgattcag
    50041 gcgattatct tacaggacca ggagagggcg ctggcccgtt tccgggaacc gattgaaggg
    50101 attcatttcg ttgactatat ggtcgagtct attgtctctc tcacccatga ggcctttggg
    50161 caacgggcgc tggtggtcga aattatggcg gaagggatgc gtaacccaca ggtcgccgcc
    50221 atgcttaaaa ataagcatat gacgatcacg gaatttgttg ctcagcggat gcgtgatgcc
    50281 cagcaaaaag gcgagataag cccagatatc aacacggcaa tgacttcacg tttactgctg
    50341 gatctgacct acggtgtact ggccgatatc gaagcggaag atctggcgcg tgaagcgtcg
    50401 tttgctcagg gattacgcgc gatgattggc ggtatattaa ccgcatcctg atcctctctc
    50461 tttccgcggg ctggtgacaa ctgagcccgc gtttcatatc gtaatttctc tgtgcaaaaa
    50521 tcatcctccc agggatcgga gaatcccacc taaatattca tcgtagcaat atatcatgag
    50581 ggtttatcgc tcccaatacg ttcgaacgaa cgttcgattg cttattttgt ggcttctgtc
    50641 aacgctgttt taaagattaa tgcgatcaat atcacgctgt gggtattgca gtttttggtt
    50701 ttttgatcgc ggtgtcagtt ctttttattt ccatttctct tccatgggtt tctcacagat
    50761 aactgtttgc aacacagaat tggttaacta atcagattaa aggttgacca gtatttttat
    50821 cttaatgagg agtcccttat gttacgtcct gtagaaaccc caacccgtga aatcaaaaaa
    50881 ctcgacggcc tgtgggcatt cagtctggat cgcgaaaact gtggaattga tcagcgttgg
    50941 tgggaaagcg cgttacaaga aagccgggca attgctgtgc cgggcagttt taacgatcag
    51001 ttcgccgatg cagatattcg taattatgtg ggcaacgtct ggtatcagcg cgaagtcttt
    51061 ataccgaaag gttgggcggg ccagcgtatt gtactgcgtt tcgatgcggt cactcattac
    51121 ggcaaagtgt gggtaaataa tcaggaagtg atggagcatc agggcggcta tacgccattt
    51181 gaagccgatg tcactccgta tgttattgcc gggaaaagtg tacgtatcac tgtttgtgtg
    51241 aacaacgaac tgaactggca gactatcccg ccgggaatgg tgattaccga cgaaaacggc
    51301 aagaaaaagc agtcttactt tcatgatttc tttaactacg ccgggatcca tcgcagcgta
    51361 atgctctaca ccacgccgaa tacctgggtg gacgatatca ccgtggtgac gcatgtcgcg
    51421 caagactgta accacgcatc tgttgactgg caggtggggg caaatggtga tgtcagcgtt
    51481 gaactgcgtg atgcggatca acaggtggtt gcaactggac aaggcaccag cgggactttg
    51541 caagtagtga atccgcacct ctggcaaccg ggtgaaggtt atctctatga actgtgcgtc
    51601 acagccaaaa gccagacaga gtgtgatatc tacctgctgc gcgtcggtat ccggtcagtg
    51661 gcagtgaagg gcgaacagtt cctgatcaac cacaaaccgt tctactttac tggctttggt
    51721 cgtcatgaag atgcggattt gcgcggcaaa ggattcgata acgtgctgat ggtgcacgat
    51781 cacgcactaa tggactggat tggggccaac tcctaccgta cctcgcatta cccttacgct
    51841 gaagagatgc tcgactgggc agatgaacat ggaatcgtgg tgattgatga aactgcagct
    51901 gtcggcttta acctctcttt aggcattggt ttcgaagcgg gcaacaagcc gaaagaactg
    51961 tacagcgaag aggcagtcaa cggggaaacc cagcaggcgc acttacaggc gattaaagag
    52021 ctgattgcgc gtgacaaaaa ccaaccaagc gtggtgatgt ggagtattgc caacgaaccg
    52081 gatacccgtc cgcaaggtgc acgggaatat tttgcgccac tggcggaagc aacgcgtaaa
    52141 ctcgacccga cgcgtccgat cacctgtgtc aatgtaatgt tctgcgacgc tcacaccgat
    52201 accatcagcg atctctttga tgtgctgtgc ctgaaccgtt attacggttg gtatgtccaa
    52261 agcggcgatt tggaaacggc agagaaggtt ctggaaaaag aacttctggc ctggcaggag
    52321 aaactgcatc agccgattat catcaccgaa tacggtgtgg atacgttagc cggcctgcac
    52381 tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg catggctgga tatgtatcac
    52441 cgcgtctttg atcgcgtcag cgctgtcgtc ggtgagcagg tatggaattt cgccgatttt
    52501 gcgacctcgc aaggcatatt gcgcgttggc ggtaacaaga aagggatctt cacccgcgac
    52561 cgcaaaccga agtcggcggc ttttctgctg caaaaacgct ggaccggcat gaacttcggt
    52621 gaaaaaccgc agcagggagg caaacaatga atcaacaact ctcctggcgc accatcgtcg
    52681 gctacagcct cggtgacgtc gccaataact tcgccttcgc aatgggggcg ctcttcctgt
    52741 tgagttacta caccgacgtc gctggcgtcg gtgccgctgc ggcgggcacc atgctgttac
    52801 tggtgcgggt attcgatgcc ttcgccgatg tctttgccgg acgagtggtg gacagtgtga
    52861 atacccgatg gggaaaattc cgcccgtttt tactcttcgg tactgcgccg ttaatgatct
    52921 tcagcgtgct ggtattctgg gtgccgaccg actggagcca tagcagcaaa gttgtgtatg
    52981 catatttgac ctacatggga ctcgggcttt gctacagcct ggtgaatatt ccttatggtt
    53041 cacttgctac cgcgatgacc caacaaccac aatcccgcgc ccgtctgggc gcggctcgcg
    53101 ggattgccgc ttcattgacc tttgtctgcc tggcatttct gataggaccg agcattaaga
    53161 actccagccc ggaagagatg gtgtcggtat atcatttctg gacaattgtg ctggcgattg
    53221 ccggaatggt gctttacttc atctgcttca aatcgacgcg tgagaatgtg gtacgtatcg
    53281 tggcgcagcc gtcattgaag atcagtctgc aaaccctgaa acggaatcgc ccgctgttta
    53341 tgttgtgcat cggtgcgctg tgtgtgctga tttcgacctt cgcggtcagc gcctcgtcgt
    53401 tgttctacgt gcgctatgtg ttaaatgata ccgggctgtt cactgtgctg gtactggtgc
    53461 aaaacctggt cggtactgtg gcatcggcac cgctggtgcc ggggatggtc gcgaggatcg
    53521 gtaaaaagaa taccttcctg attggcgctt tgctgggaac ctgcggttat ctgctgttct
    53581 tctgggtttc cgtctggtca ctgccggtgg cgttggttgc gttggccatc gcatcaattg
    53641 gccagggcgt taccatgacc gtgatgtggg cactggaagc tgataccgta gaatacggtg
    53701 aatacctgac tggcgtgcga attgaagggc tcacctattc actattctca tttacccgta
    53761 aatgcggtca ggcaatcgga ggttcaattc ctgcctttat tttgggatta agcggatata
    53821 tcgccaatca ggcgcaaacg ccggaagtta ttatgggcat ccgcacatca attgccttag
    53881 taccttgcgg atttatgcta ctggcattcg tcattatctg gttttatccg ctcacggata
    53941 aaaaattcaa agaaatcgtt gttgaaattg ataatcgtaa aaaagtgcag cagcaattaa
    54001 tcaacgatat cactagttaa tattcaataa aaataatcag aacatcaaag gtgcaactat
    54061 gagaaaaata gtggccatgg ccgttatttg cctgacggct gcctctggcc ttacctctgc
    54121 ttacgcggcg caactggctg acgatgaagc gggactacgc atcagactga aaaatgaatt
    54181 acgcagggct gataagccca gtgctggcgc ggggagagat atttacgcat gggtataggg
    54241 aggattgctc gatttcaata gtggttatta ttccaatatt attggcgttg aaggcggggc
    54301 gtattatgtt tataaattag gtgctcgtgc tgatatgagc acccggtggt atcttgatgg
    54361 tgataaaagt tttggcttta ccctgggggc agtaaaaata aaacccagtg aaagtagtct
    54421 gcttaaatta ggtcgcttcg ggacggatta tagttatggt a
//
LOCUS       DAZFFE010000019        52660 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFFE010000019 DAZFFE010000000
VERSION     DAZFFE010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.019"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            132..1829
                     /gene="ypdA"
                     /locus_tag="ACHJ69_001346"
     CDS             132..1829
                     /gene="ypdA"
                     /locus_tag="ACHJ69_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO5014371.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAMTNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1844..2578
                     /gene="ypdB"
                     /locus_tag="ACHJ69_001347"
     CDS             1844..2578
                     /gene="ypdB"
                     /locus_tag="ACHJ69_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO5014372.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2591..3448
                     /gene="ypdC"
                     /locus_tag="ACHJ69_001348"
     CDS             2591..3448
                     /gene="ypdC"
                     /locus_tag="ACHJ69_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5014373.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPNSLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHDVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(3451..5946)
                     /gene="ptsP"
                     /locus_tag="ACHJ69_001349"
     CDS             complement(3451..5946)
                     /gene="ptsP"
                     /locus_tag="ACHJ69_001349"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO5014374.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTLAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRVIPASAQDSTLLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRHLMAEQHQGLGAAIISNMEQ
                     ICAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDKKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTAAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFAPEED
                     VRPLLALENIFVDQSFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(5971..7008)
                     /gene="ypdE"
                     /locus_tag="ACHJ69_001350"
     CDS             complement(5971..7008)
                     /gene="ypdE"
                     /locus_tag="ACHJ69_001350"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO5014375.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSCDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLIAWIETVAAEIGVPLQSD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(7008..8093)
                     /gene="ypdF"
                     /locus_tag="ACHJ69_001351"
     CDS             complement(7008..8093)
                     /gene="ypdF"
                     /locus_tag="ACHJ69_001351"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO5014376.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDAMHTLATIVRQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     CEIAAELEWFMRRQGAEKASFDTIVASGWRGALPHGKASDKIVTAGEFVTLDFGALYR
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGYGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(8109..9356)
                     /gene="fryC"
                     /locus_tag="ACHJ69_001352"
     CDS             complement(8109..9356)
                     /gene="fryC"
                     /locus_tag="ACHJ69_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO5014377.1"
                     /translation="MAIKKRSATVVHGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9378..9704)
                     /gene="fryB"
                     /locus_tag="ACHJ69_001353"
     CDS             complement(9378..9704)
                     /gene="fryB"
                     /locus_tag="ACHJ69_001353"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO5014378.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9923..10888)
                     /gene="glk"
                     /locus_tag="ACHJ69_001354"
     CDS             complement(9923..10888)
                     /gene="glk"
                     /locus_tag="ACHJ69_001354"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO5014379.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEGIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            11092..12348
                     /gene="yfeO"
                     /locus_tag="ACHJ69_001355"
     CDS             11092..12348
                     /gene="yfeO"
                     /locus_tag="ACHJ69_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO5014380.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIMVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSNEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHEMMHQMKNPVFVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12463..12789
                     /gene="ypeC"
                     /locus_tag="ACHJ69_001356"
     CDS             12463..12789
                     /gene="ypeC"
                     /locus_tag="ACHJ69_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO5014381.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12930..14168)
                     /gene="mntH"
                     /locus_tag="ACHJ69_001357"
     CDS             complement(12930..14168)
                     /gene="mntH"
                     /locus_tag="ACHJ69_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO5014382.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGRGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDGKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14504..15706
                     /gene="nupC"
                     /locus_tag="ACHJ69_001358"
     CDS             14504..15706
                     /gene="nupC"
                     /locus_tag="ACHJ69_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO5014383.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15755..17944)
                     /gene="pdeA"
                     /locus_tag="ACHJ69_001359"
     CDS             complement(15755..17944)
                     /gene="pdeA"
                     /locus_tag="ACHJ69_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO5014384.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRLISPLAVIHSNYIFL
                     AWMPLCVMLSILFIFGWRGVVPILCGMFCTNLWNLHLTFLQTAVMIGSQAFAVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVAPIGIKCSMYLVGNFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLIMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGHFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYYISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(18151..18226)
                     /locus_tag="ACHJ69_001360"
     tRNA            complement(18151..18226)
                     /locus_tag="ACHJ69_001360"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18191..18193),aa:Ala,seq:ggc)
     gene            complement(18266..18341)
                     /locus_tag="ACHJ69_001361"
     tRNA            complement(18266..18341)
                     /locus_tag="ACHJ69_001361"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18306..18308),aa:Ala,seq:ggc)
     gene            18562..18921
                     /gene="yfeC"
                     /locus_tag="ACHJ69_001362"
     CDS             18562..18921
                     /gene="yfeC"
                     /locus_tag="ACHJ69_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO5014385.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18923..19315
                     /gene="yfeD"
                     /locus_tag="ACHJ69_001363"
     CDS             18923..19315
                     /gene="yfeD"
                     /locus_tag="ACHJ69_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO5014386.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVETQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(19366..20781)
                     /gene="gltX"
                     /locus_tag="ACHJ69_001364"
     CDS             complement(19366..20781)
                     /gene="gltX"
                     /locus_tag="ACHJ69_001364"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO5014387.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLTAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            21040..21115
                     /locus_tag="ACHJ69_001365"
     tRNA            21040..21115
                     /locus_tag="ACHJ69_001365"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21073..21075,aa:Val,seq:tac)
     gene            21160..21235
                     /locus_tag="ACHJ69_001366"
     tRNA            21160..21235
                     /locus_tag="ACHJ69_001366"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21193..21195,aa:Val,seq:tac)
     gene            21282..21357
                     /locus_tag="ACHJ69_001367"
     tRNA            21282..21357
                     /locus_tag="ACHJ69_001367"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21315..21317,aa:Val,seq:tac)
     gene            21362..21437
                     /locus_tag="ACHJ69_001368"
     tRNA            21362..21437
                     /locus_tag="ACHJ69_001368"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21395..21397,aa:Lys,seq:ttt)
     gene            complement(21548..22432)
                     /gene="xapR"
                     /locus_tag="ACHJ69_001369"
     CDS             complement(21548..22432)
                     /gene="xapR"
                     /locus_tag="ACHJ69_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFO5014388.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVVFRPLKERIPADLYIVY
                     ETQQVTPALVKLLAALAQ"
     gene            complement(22473..22631)
                     /locus_tag="ACHJ69_001370"
     CDS             complement(22473..22631)
                     /locus_tag="ACHJ69_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFO5014389.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(22684..23940)
                     /gene="xapB"
                     /locus_tag="ACHJ69_001371"
     CDS             complement(22684..23940)
                     /gene="xapB"
                     /locus_tag="ACHJ69_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFO5014390.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQVGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            complement(24001..24834)
                     /gene="xapA"
                     /locus_tag="ACHJ69_001372"
     CDS             complement(24001..24834)
                     /gene="xapA"
                     /locus_tag="ACHJ69_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFO5014391.1"
                     /translation="MYQAHFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGVPVACMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            25083..25847
                     /gene="yfeN"
                     /locus_tag="ACHJ69_001373"
     CDS             25083..25847
                     /gene="yfeN"
                     /locus_tag="ACHJ69_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO5014392.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(25886..26812)
                     /gene="yfeR"
                     /locus_tag="ACHJ69_001374"
     CDS             complement(25886..26812)
                     /gene="yfeR"
                     /locus_tag="ACHJ69_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5014393.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAQALWDVVRDQGNALMAGREGDPLYQI"
     gene            26902..27900
                     /gene="yfeH"
                     /locus_tag="ACHJ69_001375"
     CDS             26902..27900
                     /gene="yfeH"
                     /locus_tag="ACHJ69_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO5014394.1"
                     /translation="MKLFRIPDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(27897..28115)
                     /gene="ypeB"
                     /locus_tag="ACHJ69_001376"
     CDS             complement(27897..28115)
                     /gene="ypeB"
                     /locus_tag="ACHJ69_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO5014395.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGHRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKNEGLTQLVQPLKRPL"
     gene            complement(28117..30132)
                     /gene="ligA"
                     /locus_tag="ACHJ69_001377"
     CDS             complement(28117..30132)
                     /gene="ligA"
                     /locus_tag="ACHJ69_001377"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO5014396.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPEPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDETEMLRLLGS"
     gene            complement(30203..31201)
                     /gene="zipA"
                     /locus_tag="ACHJ69_001378"
     CDS             complement(30203..31201)
                     /gene="zipA"
                     /locus_tag="ACHJ69_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO5014397.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANALEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPTQPVQQPVQQPAYQPQPEQPLQQPVSPQVASAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVTDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQACFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANV"
     gene            31431..32192
                     /gene="cysZ"
                     /locus_tag="ACHJ69_001379"
     CDS             31431..32192
                     /gene="cysZ"
                     /locus_tag="ACHJ69_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO5014398.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVLQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPKLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            32377..33348
                     /gene="cysK"
                     /locus_tag="ACHJ69_001380"
     CDS             32377..33348
                     /gene="cysK"
                     /locus_tag="ACHJ69_001380"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO5014399.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKLGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            33732..33989
                     /gene="ptsH"
                     /locus_tag="ACHJ69_001381"
     CDS             33732..33989
                     /gene="ptsH"
                     /locus_tag="ACHJ69_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO5014400.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            34034..35761
                     /gene="ptsI"
                     /locus_tag="ACHJ69_001382"
     CDS             34034..35761
                     /gene="ptsI"
                     /locus_tag="ACHJ69_001382"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO5014401.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            35802..36311
                     /gene="crr"
                     /locus_tag="ACHJ69_001383"
     CDS             35802..36311
                     /gene="crr"
                     /locus_tag="ACHJ69_001383"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO5014402.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(36354..37205)
                     /gene="pdxK"
                     /locus_tag="ACHJ69_001384"
                     /gene_synonym="thiJ"
     CDS             complement(36354..37205)
                     /gene="pdxK"
                     /locus_tag="ACHJ69_001384"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO5014403.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKDCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            37310..37678
                     /gene="yfeK"
                     /locus_tag="ACHJ69_001385"
     CDS             37310..37678
                     /gene="yfeK"
                     /locus_tag="ACHJ69_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO5014404.1"
                     /translation="MKKIICLVVTLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFVDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(37681..38592)
                     /gene="cysM"
                     /locus_tag="ACHJ69_001386"
     CDS             complement(37681..38592)
                     /gene="cysM"
                     /locus_tag="ACHJ69_001386"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO5014405.1"
                     /translation="MSTLEQTIGNTPLVKLQRMAPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(38727..39824)
                     /gene="cysA"
                     /locus_tag="ACHJ69_001387"
     CDS             complement(38727..39824)
                     /gene="cysA"
                     /locus_tag="ACHJ69_001387"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO5014406.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGD
                     RLYVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(39814..40689)
                     /gene="cysW"
                     /locus_tag="ACHJ69_001388"
     CDS             complement(39814..40689)
                     /gene="cysW"
                     /locus_tag="ACHJ69_001388"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO5014407.1"
                     /translation="MAEVTQLKRYYARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(40689..41522)
                     /gene="cysT"
                     /locus_tag="ACHJ69_001389"
     CDS             complement(40689..41522)
                     /gene="cysT"
                     /locus_tag="ACHJ69_001389"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO5014408.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFRKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(41522..42538)
                     /gene="cysP"
                     /locus_tag="ACHJ69_001390"
     CDS             complement(41522..42538)
                     /gene="cysP"
                     /locus_tag="ACHJ69_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO5014409.1"
                     /translation="MAVNLLKKNSLVLVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMGKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(42696..43487)
                     /gene="ucpA"
                     /locus_tag="ACHJ69_001391"
     CDS             complement(42696..43487)
                     /gene="ucpA"
                     /locus_tag="ACHJ69_001391"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO5014410.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            43767..44663
                     /gene="murQ"
                     /locus_tag="ACHJ69_001392"
     CDS             43767..44663
                     /gene="murQ"
                     /locus_tag="ACHJ69_001392"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO5014411.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHTQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSSTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERSCKTAIVMVLKNLD
                     ADEAKKCLDQHGGFIRKALEKE"
     gene            44667..46091
                     /gene="murP"
                     /locus_tag="ACHJ69_001393"
     CDS             44667..46091
                     /gene="murP"
                     /locus_tag="ACHJ69_001393"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO5014412.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KARQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIECMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVSGFIFTTLFGCRNVNLD"
     gene            46094..46180
                     /locus_tag="ACHJ69_001394"
                     /pseudo
     CDS             46094..46180
                     /locus_tag="ACHJ69_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(46269..47168)
                     /gene="yfeX"
                     /locus_tag="ACHJ69_001395"
     CDS             complement(46269..47168)
                     /gene="yfeX"
                     /locus_tag="ACHJ69_001395"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO5014413.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRTLSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGYCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(47264..47839)
                     /gene="yfeY"
                     /locus_tag="ACHJ69_001396"
     CDS             complement(47264..47839)
                     /gene="yfeY"
                     /locus_tag="ACHJ69_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO5014414.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADSDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(47900..48349)
                     /gene="yfeZ"
                     /locus_tag="ACHJ69_001397"
     CDS             complement(47900..48349)
                     /gene="yfeZ"
                     /locus_tag="ACHJ69_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO5014415.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(48336..48761)
                     /gene="ypeA"
                     /locus_tag="ACHJ69_001398"
     CDS             complement(48336..48761)
                     /gene="ypeA"
                     /locus_tag="ACHJ69_001398"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO5014416.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            48975..49844
                     /gene="amiA"
                     /locus_tag="ACHJ69_001399"
     CDS             48975..49844
                     /gene="amiA"
                     /locus_tag="ACHJ69_001399"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO5014417.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNG
                     HSKPKAKKSGGRRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            49848..50747
                     /gene="hemF"
                     /locus_tag="ACHJ69_001400"
     CDS             49848..50747
                     /gene="hemF"
                     /locus_tag="ACHJ69_001400"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO5014418.1"
                     /translation="MKPDAHLVKQFLLNLQDTICQQLSAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGINFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALNEFIKVRDWV"
     gene            complement(50753..51805)
                     /gene="eutR"
                     /locus_tag="ACHJ69_001401"
     CDS             complement(50753..51805)
                     /gene="eutR"
                     /locus_tag="ACHJ69_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO5014419.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMMTAESISHQSYRRLLSRAREYMLENMSEPVTVLELCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(51851..52351)
                     /gene="eutK"
                     /locus_tag="ACHJ69_001402"
     CDS             complement(51851..52351)
                     /gene="eutK"
                     /locus_tag="ACHJ69_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO5014420.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(52364..>52660)
                     /locus_tag="ACHJ69_001403"
     CDS             complement(52364..>52660)
                     /locus_tag="ACHJ69_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFO5014421.1"
                     /translation="TAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATYGIDAA
                     LKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQRA"
BASE COUNT        12925 a        14160 c        13027 g        12548 t
ORIGIN      
        1 gatgcattgc taatacacca tttatctttc aatccccttt taaaattgtg gtttttcctc
       61 aaaaacggcc tgctggtcat cagccttcag gttacctatc atagaggttt aatccttatt
      121 cagagtcacc cgtgcacgaa atattcaaca tgctgctggc ggtcttcgat cgggccgcgt
      181 taatgcttat ctgcctgttt tttctcatcc gtatccgcct gtttcgcgaa ctgttgcaca
      241 agtcggcaca ctcaccgaaa gagctgctcg ccgttaccgc cattttctcg ctgttcgccc
      301 tgttcagcac ctggtccggc gttcccgtag aaggctcgct ggtgaacgta cgtattatcg
      361 cggtgatgtc aggcggaatt ctgtttggcc cgtgggtggg catcattacc ggcgtgattg
      421 cgggtattca ccgttattta attgacatcg gcggcgtaac ggcgatcccc tgctttatca
      481 ccagcattct ggcgggttgt atatcgggct ggatcaacct gaaaatcccc aaagcacagc
      541 gctggcgcgt cggtattctc ggcggcatgt tgtgtgaaac gttgaccatg attctggtga
      601 ttgtctgggc accaactacc gcattgggga tcgatatcgt ctctaaaatc gggattccaa
      661 tgatcctcgg cagcgtctgt atcggcttta ttgtgttgct ggtacaaagt gttgagggag
      721 aaaaagaggc cagcgccgcg cggcaggcca agctggcgct ggatatcgcc aacaaaacgc
      781 tgccgctgtt tcgccatgtc aatagcgagt cattacgcaa agtctgcgaa attatccgcg
      841 atgacattca cgccgatgcg gtggcgatga ctaataccga tcatgtgctg gcctacgttg
      901 gcgtgggtga acataactat cagaatggcg atgacttcat tagcccgact acccgccagg
      961 cgatgaatta tggaaaaatc atcattaaaa acaatgatga agcccaccgc acaccagaga
     1021 ttcattccat gctggtgatc ccattgtggg agaaaggcgt tgtgaccgga acgctgaaaa
     1081 tttactactg ccacgcgcat cagataacct cgtcattaca ggaaatggcg gtcggtctgt
     1141 cacaaatcat ctccacgcaa ctggaggtgt cacgcgccga gcagctgcgt gaaatggcaa
     1201 ataaggcaga gcttcgcgcc ctgcaaagca aaattaatcc ccatttcctg tttaacgctt
     1261 tgaacgctat ttcatcgtca atccgcctga atccggatac tgctcgccag ctgatcttta
     1321 atctgtcgcg ttatctgcgc tataacattg aattaaaaga tgatgagcaa atcgatatca
     1381 aaaaagagct gtatcaaatt aaagactata ttgccattga gcaggcccgc tttggcgaca
     1441 agctgacggt tatctatgat attgatgaag aggtgaattg ctgcattccc agcctgctga
     1501 tccagccgtt ggtggaaaac gccattgtcc acggtatcca gccttgcaaa ggtaaaggcg
     1561 ttgtcaccat cagcgttgca gagtgcggta atcgggtacg tattgcggtg cgagataccg
     1621 ggcacggcat cgatccaaag gtgattgagc gggtcgaagc gaacgaaatg ccgggcaata
     1681 aaattggcct gctgaatgtc catcatcgcg tgaagttatt gtatggcgag gggctgcata
     1741 tccgccgcct ggaaccagga acggaaattg cgttttacat tcctaaccaa cgcaccccag
     1801 tcgcctcaca ggctacgtta ttgctttgag ccggagtgat attgtgaaag tcatcattgt
     1861 tgaagacgaa ttcctggcac aacaggaact gagctggcta attaaagagc acagccagat
     1921 ggagattgtc ggcacctttg acgacggtct ggacgtgttg aagtttttgc agcataaccg
     1981 cgtcgacgcc atttttctgg atatcaatat tccgtcgctg gatggtgtgt tgctggcaca
     2041 aaacatcagc cagttcgccc ataaaccgtt tattgtgttc atcaccgcgt ggaaagaaca
     2101 tgcggtagaa gcgtttgaac tggaagcgtt tgactacatt ctcaaaccgt atcaggagtc
     2161 acgcattacc gggatgctgc aaaaactgga agcggcctgg caacaacagc agaccagcag
     2221 tacgcctgcc gcgacggtaa cgcgtgagaa tgacaccatt aatctggtga aagatgagcg
     2281 aatcatcgtc acgcccatta acgatatcta ttacgccgaa gcgcatgaga aaatgacctt
     2341 tgtctatacg cggcgtgaat cctacgtaat gccgatgaac attaccgagt tttgcagcaa
     2401 gctgccgccg tcgcattttt tccgctgcca tcgctcattt tgtgtcaatc tgaacaaaat
     2461 ccgcgagatc gaaccgtggt ttaataacac ctatattctg cggctgaaag atctggattt
     2521 tgaagtgccg gtcagccgca gcaaagttaa agaattccgc cagttaatgc atctttaatc
     2581 gggaactttc atgaaagcac caggcttgcc cgccgatcaa caatttttcg ccgatctgtt
     2641 cagcggcctg gtgcttaacc cgcaactact ggggcgcgtc tggtttgcca gccagcctaa
     2701 ctcattgcca gtgggcagtt tatgtattga ttttccccgt ctggatatcg tgctacgcgg
     2761 cgaatacggc aatctgctgg aagcaaagca gcaacgtatg gtggaaggag aaatgctgtt
     2821 tattccggca cgcgcggcta atttaccggt caacaacaaa ccggtgatgc tgttaagcct
     2881 ggtgttcgca ccgacctggc ttggcttatc attttacgat agccgcacca cgtcgttgtt
     2941 acatcctgct cgccagatcc aacttcccag cctgcaacgc ggcgaaggtg aagcgatgct
     3001 taccgccctc acccatctta gccgttcgcc gctggagcag aatatcattc agccactggt
     3061 gttaagtttg ctgcatcttt gccgtaacgt ggtgaatatg ccgccgggca attcgcagcc
     3121 gcgcggcgat tttctctatc acagcatttg taactgggtt caggataatt atgcccagcc
     3181 gctcacccgc gagagcgtgg ctcagttttt taatatcacg cccaatcatc tgtcaaaact
     3241 gtttgctcag catggaacaa tgcgttttat cgagtatgtt cgctgggtac gaatggcaaa
     3301 ggcaaggatg attttgcaga aatatcatct gtcgattcat gatgtggcac agcgttgcgg
     3361 ttttccggac agcgactatt tttgtcgcgt tttccggcgt cagtttggcc tgacgccggg
     3421 agagtacagc gcccgttttc agggctaacg tcagaaggtt aattccgttt ccagcagcgt
     3481 caggatgctt tgcgcatctt gcgcggcaaa caacgactgg cggaagtttt tattcaccag
     3541 tttgcgcgcc agctgcgaga agactttcac atgattcatc ccttcgttag cgcccagcgt
     3601 cagcatgatc accagttcga cttcgcccat ttctgactgc cagtcaaccg gtttcaccaa
     3661 ccgggcaatg ctgatactgg aatgacggat ccactgtgat ttggtgtgcg ggatcgccac
     3721 gccaaaacca acgccagtag taacaatctc ttcccgctgc cagacatctt cttccagctc
     3781 gaacggatgt tcagtacgcc cgttaacacc gaggttaccg cacaggaact ggatcgcctg
     3841 ctctttatta ctaaaggact gatcaacaaa gatattctct agtgccagca gtgggcgaac
     3901 gtcttcttcc ggcgcaaacg cggtaagtaa cgcttcaatt tcctgcgcgc tgcggcactc
     3961 gcacgcctga cgcgccagtt cccgacacgc ctcgctatcc agttgacgaa gctggctttt
     4021 cactgccgga atacgcgggc tactcatgct tagctcatcc aggcccagcc ctagcagtag
     4081 cggcagataa cgactttcac cgcccagttc accgcaaata cctacccatt tgccgcgctg
     4141 atgcgcagcg gtaacaatct gctgcaacat gcgcaggaac gatggcgtaa tcgggttata
     4201 cagcggcgat acgcgcgggt tattacgatc gaccgcatac agatactggg tcatatcgtt
     4261 ggagccgata ctgaagaaat cgacctcatc gcagaagtgg tcgatgatgt agcacaccga
     4321 cggcacttcc accatgatac ccagcgtaat cgtctctgca tgacgcaggc catcgcgctt
     4381 aagctcaacg atcgcttttt ggatctcacc cttcacccat aagatctgat cgagactgtg
     4441 gaccattggg atcatcaact gggcgttgcc gaaactggca gcgcgcaaaa tcgcccgcaa
     4501 ttgagtacgg aacaggccag caaattccgg gtaaatacgt accgcgcgat agccgaggaa
     4561 cgggttctct tcctggggaa tgttgaggta aggaatgctt ttatcgccac cgatgtccat
     4621 cgtgcggaag ataatcggct tgtcgcccgc cgccagtagc acctgctggt acgcttcaaa
     4681 ctgttcctgc tcgtccggcg cgctgtcgcg atccatatag agcatttcgg tacgaaacag
     4741 cccaacgcct tccgcgccgt tggcaaacgc gcctggggct tccagagctg tgccaatatt
     4801 cgccgcaata tcaatacgct ttttatcacg ggaataggcc agctgcgcgg cagcttgcgc
     4861 ctgttgtttt tggcgtttat ccgccagcgt ctgcgccacc tggtaataac cgctcaccgc
     4921 atcgttggga ttaattgcca gtacgccgca ctgggcatca agcactgcag gttgcccggc
     4981 ataacgggca atcgcatcca gcggcaggcc gctcagtacc gggatcgccg aagcgcgggc
     5041 cagaatcagg gtatgcgagg tgcgcccggt tttctccaga atcatgcccg caagattttt
     5101 taaatcgagg ctcaaaaact ggcttggggt taaatcttca gccaccagaa tggtcggttt
     5161 ttcaagcacc agattattgc gcggttttag ttccggccag gtgatatgca gcaactgttc
     5221 gctgatatcg cgaatgtcgc tgacccgctc gcgcagataa tcgctggcag aagcagaaag
     5281 tttggcacaa atctgctcca tattgctgat gatcgccgcc cccagcccct gatgctgttc
     5341 tgccatcagg tgacggatat tgcctgcaaa ttcatcatcc tgaatcagcg acaaatgggc
     5401 gctgaggata gttttgcttt cgccgtcacg ctcacgcaat tgctggttca gttgctcggc
     5461 aagcgttgcc agactgtgct ccagtagggt ggaatcttgc gcactcgcag ggatcacccg
     5521 ataactgtcg aggctgtcgc tctgtaacag ggtcagtgta cccacgccaa cgccgcttgc
     5581 cagcacgttg ccgtacagta aatccgggtt caggcggctt aatgaacgcg gcagcggatg
     5641 cgccgtcagt tctgccagcg tcggctggac gctatcgctg tcgataaagc gcacctggat
     5701 atactcttcc agcactcgcc gcgcctgctc ttcatcgcta ccgctaatgt tcaggctgca
     5761 actgtcatta aataaagtgc cagtgccaat cagcgccagc gagcttttcg catcggcctt
     5821 tgcgttctgg cgatggttaa taaaagtgat ttcgctttgc cactggctgc actgctcttt
     5881 aagttcccac gccggacgag catgtagacc gttaggcaaa ggacagagaa attgaatcgt
     5941 taacataacc gctccgggtt aatcaggaga tcatctgaaa tccgtcagtt gaacaaccgt
     6001 ctcacgggta agacgttgaa taagggcaga taaaagttgc tgcatctgca aaatgtcgcg
     6061 gcagtcggca atcgatgcgg cgcaatgtcc gtggcgggtt gctggcccca tcaccactgt
     6121 gggtacgcca gtgccggtta agtgcaccgc cccaccgtcc gtgccgccat tgctgaacat
     6181 atctgactgc aacggtacgc caatttctgc cgccacggtt tcgatccagg cgataagttt
     6241 tggcggcgca atcagtgact tgtcgcttaa caccagcatc ggcccgttac caatctggcg
     6301 atggttagct gcgccataat caaagttttt cgcccagcag gcggtatcaa gcacaatggc
     6361 gacatccggc gacaccgcgc gggtggcagt ttgcccgccg cgtaatccca cctcttcgct
     6421 ggaacttgcc accagccaca cttccgcagg cagttcggcg tcgtgcagct cacgcagtaa
     6481 cgtcaccagc agatagcaac cgaggcggtc atcaaaggct ttccccatca cccgctggtg
     6541 agggagaacc tgaaaagtgg tatcgaaggt aacgcgatcg cctggacgaa ttcctgcctg
     6601 catcacttcg tcacaggagc gcgcgccaat gtccacgcgc atggcgctga cgtcattccc
     6661 ctgccgatcg ccgtcaagca ggcctggaat tttgcactct tcacgggtag tgatacgcac
     6721 cgactgaagc tggcgggcag ccatgcgtac attgccaacc ggcaacacat cgatcgcccc
     6781 ttcgcgggag atgctgcgca ccataaatcc cacttcgtcc atatgcgcac agatcatcac
     6841 cttcggacct gtcgactcat tcaggcggat cagcaccgat cccagaccat caaatcgcac
     6901 ttctttttgc aggcgatccg cttcttccag caggatctgc cgcacttcct gttccgagga
     6961 ggcgatcgca tctgcctcgc tcaacgcttt taatagcgat aaatccatta tgcctctccc
     7021 gtgagcaaca ctgttttcgg catggcgtag agcacctctg cgccttgcgg ggtaaccagc
     7081 acaacatctt cgatgcgcac gcccccttgc cctggcaaat aaatccctgg ctccacggtc
     7141 agtaacatac ctggctgtag cgtcgtggtg tcccgcggtg aaaaacgcgg atcttcatgg
     7201 acttcaatgc cgatagcatg accggtgtta tggccgaaat agtcgccata accggcttct
     7261 gtaatcaccc ggcgcgcggc atcgtcaacc tgctggcagc gcacgccggg gcgaatcgcg
     7321 gagattgctg cgagctgtgc ctgcaggaca atttgataga cgttaaacag cgggtgagat
     7381 tcggcgctca ccccttcgcc attcaccagc aaggtgcgcg tcatatcaga gcagtagccc
     7441 cgatagagcg caccgaaatc gagagtgaca aactcgcccg ctgtaacaat cttgtcgctg
     7501 gctttgccgt gcggcagcgc cccacgccag ccgctggcga caatggtgtc aaaagaggct
     7561 ttttctgcgc cctgccggcg cataaaccac tccagttcag cggctatctc gcattcgctc
     7621 attcccgcct gaataaagcg gcgaatatgc tctgcgccac gatcggcaat cccgcaggca
     7681 aggcggattt tctccacctc ttctggcgtt ttgatttgcc gcagcacatc cggcgtggcg
     7741 ctcaccagtt tcgcattgag ttcggactgc cagcggtgcg cggtttccca actcacctgc
     7801 tggccttcaa aaccgagcgt ttgcaactgc tcatcggcaa tgatttgtct gacgatggtg
     7861 gcaagcgtgt gcatcgcgtc gagcaaatgc agctggtaac cttgcgtgcg ggcttctaca
     7921 tccgcgtaat agcgcgaatc caccagaatg tgcgcacttt cacggctaat caccacataa
     7981 cctgatccgg tggagatccc cagatgcggc tgtttgttct gccgtgagga gagaagcacc
     8041 gcatccagtt gttgtgcctt aagccagtca cgcagcgaag cgagtaatgt catctggatt
     8101 tttccttctt acaggctatc gattaacaat ttgcctttac ggaacatcat cagacgcagg
     8161 aacaccacca tcagcgcagt tatgactgcc cccagcgcga tccccgccat gtagacgccg
     8221 aggttagtga ccagcggcca tgcccagata gcggactccg gaaaccattg cactgcgccc
     8281 agccagacag cggcggttga gccgacaatc gcgccgacca tatacgacgg aatggcggtg
     8341 atagggcttt ccagtgcaaa cggaatcgca ccctcactga tccccataaa cgccaggaac
     8401 atcgcggttt taccctgcgg ataaagctgg gcgttgaaca agcgtttgcc agttaagcga
     8461 cgatcgataa tggtcgccag acccagacca atcggcggaa taacgatagc gatagaacgt
     8521 gcagtgaccg gtaatacgtg gtcagtggta aagctgaagg caacgaaacc tgccgctttg
     8581 ttgatcgggc caccaaggtc gattgctgtc gccgcggcga tccccattgc gtacatcagc
     8641 gcgcccttct cacctgcagc ggtcagcacg gtacggatac cgccgttgat ccagccacca
     8701 aagggggtga tgacgtagta catcgccagc atgacaaaaa tggcggaaag aatcggtagc
     8761 aaaaatgtgg ttttgaacgc taacaggaaa tccggtaact ggattttctg gttcatccac
     8821 ttcacgaggt aaccggcaac gatagaaata atcagcgcac caatgaaggt ggacggcacc
     8881 ggcgaagagg tcgcccattg catcgtgctg ggatcgaagt tcagcagttg cgtcggctgg
     8941 gtagacatca agccgccgat aaatccagcc gggaacgcca gtttgccacc gatagagttc
     9001 gccacaaaag cagcaaacat cggaatggca aaaccaaaca acacaccgcc gaaggactgc
     9061 gacagccagg caaatttcag taaggagagg tcgaaaccgg agaatttccc actattaaga
     9121 gcatccatga tgccgatatc agcaggaatt ttaagccagc tataagcaat cagctgactg
     9181 aaagcgagga tcacaccgcc cataattaag gtcggcacca tgcgtgaaat ccctgacatc
     9241 acatgctgcg ggagttcacc ccagaagctg gatttagagg cctgcggatt cttaactgcc
     9301 gctgccgcac cggatgcgcc atgcacaacg gttgcactgc gttttttaat ggccataaca
     9361 attatccctg tcgaatatta ctgttgttca gaggcaatca tctcttcgat ttctttgatg
     9421 attcccgcag cgtttttaat tgcgtcctgc aaagtgattt cataaacgtc gcgtgattcg
     9481 aaacgttcgt tatcttccgg ggtaactgcc acggagtgga tgatgatggt cgcttcggcg
     9541 atatcctgcg ccgtcaggcg attctggata ccgtccgcgc cctgggtttc aattttcact
     9601 tcataaccgg cttctaccgc cgcttcttcc agcgcctgag cggccataaa agtgtgagcc
     9661 aggcccattg ggcaggcaca taaggcaatc agtttcttac tcatcgttaa ttcctcgttc
     9721 agtgatagtt gacgagtgaa attatgaacg tgcaaaacaa atcgccgtta ccagacaaat
     9781 aatgcattta ctggagaatt aatcccgctg tacggaagtg tgacgcaggt cggccttgtg
     9841 agggggacac aaagaaaatt taattaactg tatgatttaa aagattatcg ggagagttac
     9901 ctcccgatat aacaggaagg atttagagaa tgtgacctaa ggtctggcgt aaatgtgcac
     9961 cggaaccgag aagacccgga ttgtcatgaa cgatgagata caccggaata tcatggacat
    10021 attctttaaa gcgcccttta tcttcaaatg cggcgcggaa accagaggct ttgaagaact
    10081 caaggaagcg cggcacgata ccacccgcaa taaacacgcc gccaaatgtc ccgagattga
    10141 gcgccagatt gccgccaaaa cggcccataa tgacgcaaaa cagcgacaac gcgcggcggc
    10201 aatcggtgca gctgtcagcc agcgcgcgtt cggtaatatc ttttggcttg agattttctg
    10261 gcaggcggtt gtcggcttta acaattgcgc gatacaaatt caccagtcca gggccagaaa
    10321 gcacgcgttc cgccgaaaca tgaccaattt ccgcacgcaa tatttcgagg ataatgccct
    10381 cttcttcact attcggcgca aaatcaacgt gaccgccttc tcctggcaag cttacccaac
    10441 gcttatcgac atggaccaga tgcgcaaccc caagccctgt tccggcaccg taaaccgcaa
    10501 taggcttacc ttctaccggt tctgcgccac caaactgaat cagatgctct tttttcagca
    10561 tcgggatcgc catcgataca gcagtaaaat cgttaataat ttccagatgg ctaaaaccga
    10621 gattcttttt catttcggca attgagaacg cccaggtatg gttggtcatt gccacccagt
    10681 caccggtaat tgggcaagcg atggcaatac agccgtcttt cacctcgacc ttatgttctt
    10741 caagataaac gcgaatgacc gcttcgaggc tggggtaatc aagccctgaa taggtcttag
    10801 cctgcgagat ttcaccactg gcaatatcac acagagcaag acgtgcgttg gtgccgccca
    10861 catcaccgac taatgcatac tttgtcattc ttcaactgct ccgctaaagt caaaataatt
    10921 ctttctcaca ctgtaaatac ctgggggcat aacaacaacg ccggaaaggc aggctccctg
    10981 taaatatcga tctgggtcac acaattactt tatcgtttca gcaccaattg cagcgatgcc
    11041 tttttgcaag ctgggcaaac taagtatctg accccgcata aggaatagaa catgctccat
    11101 ccgcgagcca gaaccatgtt gttattatcg ctccccgccg tggcaattgg gattgcgtcc
    11161 agtcttattc tgattatggt gatgaaaatc gcctcggtat tacagaattt gctctggcaa
    11221 cgactgccgg gaactctggg gatagcccag gattcacctc tctggatcat cggtgtatta
    11281 acgctaacgg gtattgcggt ggggttggtt atccgtttca gccagggtca tgccggacca
    11341 gaccccgcct gtgaaccgct gatcggcgca ccggttccgc cctctgcgct accaggactt
    11401 atcgtagcat taattctcgg tcttgctggc ggcgtcagcc tggggccgga acatccgatc
    11461 atgaccgtca atatcgccct tgccgtcgcc attggcgctc gtctgttacc gcgcgtcaac
    11521 cgaatggagt ggactatttt agcctctgcc ggaaccatcg gcgcgctgtt tggcacacct
    11581 gttgcggcgg cattgatatt ttcgcaaacc ttaaatggca gtaatgaagt tccgctatgg
    11641 gatcgtctct ttgcgccgtt aatggcggca gcagctggtg cacttactac cggattattt
    11701 ttccatcctc atttttcact gcccattgcg cattacggac agatggaaat gaccgatatt
    11761 ctcagcggtg caattgtcgc ggcgattgcc atcgcagcag ggatggtcgc cgtatggtgc
    11821 ttaccacggt tgcacgagat gatgcatcaa atgaaaaatc cggtgttcgt gctgggtatt
    11881 ggcggattta ttctcggtat tctgggggtt attggtggac cggtttcgct gtttaaaggg
    11941 ctggatgaga tgcagcagat ggtggcaaat caggctttca gcaccagcga ttactttttg
    12001 ctggcggtga taaaacttgc cgccctggtc gttgctgccg ccagtggctt tcgcggtggg
    12061 cgaatcttcc cggcagtgtt tgtcggcgtg gcattagggt tgatgcttca tgagcacgtt
    12121 cccgccgttc cagcggcaat aaccgtttcc tgcgctatac tcggcatcgt gctggtggta
    12181 acacgcgatg gctggttaag tctttttatg gcggcagtcg ttgtacccaa taccacattg
    12241 ctaccgctgc tctgtatcgt catgctcccg gcatggctgt tattagcagg taagccgatg
    12301 atgatggtca atcgtccgaa gcaacagcca ccccacgata acgtttagca aaaaatgctc
    12361 tcctttatga tttaagagtt atggcgatga tacggcgctt tacctccccg taatattgcg
    12421 ttaacaggcc gctgacggct tcgatcgtga aggagaataa caatgttcag gtcactgttt
    12481 ctggcggccg ccctgatggc atttaccccg cttgcggcaa acgcaggtga aatcacccta
    12541 ctgccatcaa tcaaattaca aattggcgat cgcgatcatt acggtaatta ctgggacggt
    12601 ggtcactggc gcgaccgtga ctactggcac cgcaattatg aatggcgtaa aaaccgctgg
    12661 tggcgtcatg ataatggcta ccatcgtggc tgggataagc gtaaagcgta tgaacgtggc
    12721 tatcgtgaag gctggcgcga tcgtgacgat catcgtggaa aaggccgcgg acatggacac
    12781 cgccattaaa cgcatccgtc aatggagcac aatgcctgat gcgacgctag cgcgtcttat
    12841 caggcctaca aaccagctct ttaacgcgta gggcggataa agcatttacg ccgcatccgc
    12901 caatggtgcc aaatgcgacg ctcattcaac tacaatccca gtgccgttcc taccagtaac
    12961 cagatattca gcgccactac cagcaccaca atcacccagc ctgtctgttt tacgcgttta
    13021 ctgttcacca gatcgcccat caacttgccg tcactggtga aaatcagcag aggaaccagc
    13081 gccagggcga taccaaaact taacagcacc tgactcataa ccagaatccg tgtcggatct
    13141 aatcccatca gaatgacaat aaatgacggc agcatggtga ctgtacgacg cacccacagc
    13201 gggatatgaa agcgaatgaa gccctgcatc actacctgcc ccgccagcgt ccccaccacc
    13261 gttgaagaca gtccggcagc aaccaggctt aatccaaaga ccgttgccgc agcatggctt
    13321 aacagcggtt gcagcgtcag ataagcctca tcaagatcgg caacaccagt atgaccagaa
    13381 aagtggaacg ccgccgcagc tgtagccatc atcgccagat tgacaaaacc ggcaatcgtc
    13441 atggcgatag ccacatccca tttggtggcg gaataacgtt gttgacgcga accgccatgt
    13501 aaatgctgag tgagcgaaga gtgcaaataa atcacatgcg gcataatcgt cgcccctaac
    13561 acgcccgctg ccaggaagac cgcctccgaa gtaggtaaac tcgggattac cattcctcta
    13621 cccagctgcg ccaggttagg ctgggagaaa atcaactcga caatgtaagc agcggcaaca
    13681 aacaacagta acccgccaat cactttctcc agcggttttt gcccgcgacg ttgcagcatt
    13741 aaaataagga aagtcgcgat tcccgtcagc accgcgccct gcaacaacga aacaccaaga
    13801 atgagtttaa aaccgatcgc cgcaccaata aattccgcca ggtcggttgc catcgcaata
    13861 atttctgcct gaacccaata gaaccacacg acgggacgcg ggtagtgatc gcgaatctgc
    13921 tccgccagat ttttaccggt ggcaatcccc agtttggccg agaggatttg aatcagcatc
    13981 gccatcaggt tagcccaaac gacaacccac aataactgat agccgaagct ggcacccgcc
    14041 tgaatattgg tcgcaaagtt accgggatcg atatagccaa tcgccgcaat gaacgcaggt
    14101 cccattaatg cgagcctcat cttgcgcgcc gcccgtccgc tgctactctc aacgcgatag
    14161 ttcgtcatct tgtgcctcta aaacatagcc tttgctatgt ttcatgctac gttaaatgag
    14221 aatgattatc aaattcattt aaatgggttg tgatgatttc tctgatagac caggattatg
    14281 actacgaaaa gatagatgac tcgaatgtta tggttatgtt taatattagc acatttacat
    14341 aaccttccgc ttccatacac aacatagcag aaatgtatga cagatcacta tttttgaagc
    14401 ttgtcacagg acgtcattat agtgtgtgtc agatctcgtt ttccttaacc atgttacata
    14461 gaatgtgcac ggaaatttaa cctgcctcat atttggagca aatatggacc gcgtccttca
    14521 ttttgtactg gcacttgccg ttgttgcgat tctcgcactg ctggtaagca gcgaccgcaa
    14581 aaaaattcgt atccgttatg ttattcaact gcttgttatc gaagtgttac tggcgtggtt
    14641 cttcctgaac tccgacgttg gtctgggctt cgtgaaaggc ttctccgaaa tgttcgaaaa
    14701 actgctcggt tttgccaacg aagggactaa cttcgtcttt ggtagcatga atgatcaagg
    14761 cctggcattc ttcttcctga aagtgctgtg cccaatcgtc tttatctctg cgctgatcgg
    14821 tattctccag catattcgcg tattgccggt gattatccgc gcaattggtt tcctgctctc
    14881 caaagtcaac ggcatgggca aactggaatc ctttaacgcc gtcagctccc tgattctggg
    14941 tcagtctgaa aactttattg cctataaaga tatcctcggc aaaatctccc gcaatcgtat
    15001 gtacaccatg gcagcaacgg cgatgtccac cgtgtcgatg tccatcgttg gtgcatatat
    15061 gaccatgctg gagccgaaat acgtcgttgc ggcgctggta ctgaacatgt tcagcacctt
    15121 tatcgtgctg tcgctgatca acccttaccg tgttgatgcc agtgaagaaa acattcagat
    15181 gtccaacctg cacgaaggtc agagcttctt cgaaatgctg ggtgaataca ttctggcagg
    15241 tttcaaagtt gccattatcg ttgccgcgat gctgatcggc tttatcgccc tgatcgctgc
    15301 actgaacgct ctgtttgcta ccgtgactgg ctggtttggc tacagcatct ccttccaggg
    15361 catcctgggt tacatcttct atccgattgc atgggtgatg ggtgttcctt ccagtgaagc
    15421 actgcaagtg ggcagtatca tggcgaccaa actggtttcc aacgagttcg ttgcgatgat
    15481 ggatctgcag aaaattgctt ccacgctctc tccgcgtgcg gaaggcatca tctctgtgtt
    15541 cctagtttcc ttcgctaact tctcttcaat cgggattatc gcgggtgcgg ttaaaggcct
    15601 gaatgaagag caaggtaacg tggtttctcg cttcggtctg aaactggttt acggctctac
    15661 cctggtgagt gtgctgtctg cgtcaatcgc agcactggtg ctgtaagacc atacataaaa
    15721 aagccggggt aattccccgg cttttttacg cctgttaatc agctaatggc tgcgggcgac
    15781 caatcaaata cccttgcaga tattgcaccc cgagcttatg caatagcgcc tgctgctgtt
    15841 gcgtctcgac aaactccgcg accacactca atgacttcgc tttcgccaga tcggtaattg
    15901 atctcacaat catcgcatcc agcgtgttcg tgacgatatc tttcacaaag acgccatcaa
    15961 ttttgatgat atcagcctgc aaacgcttta accgttcgta gttggcatat ccggtgccaa
    16021 agtcatcaat cgcaatccgg aaaccaaact tatgcagctg ctcgatgttg tacatgctgc
    16081 tttctgcgtt agaaaacgcc tgctcctcgg tgatctcaag aatgaccgcc tgcggggaga
    16141 tgtaataacg cttaaacaga cgaataatcc gcccggcaat attcttttgc agcagcgtga
    16201 gcggcattaa attgactgaa aagcgtggac cttttttgtc gcaagggtgt gttgccagcc
    16261 acttcaacaa ggattccagc acttgcaaat caaaacgcgc gctaaggtta aactgagcaa
    16321 taaggggcag aaacttatcc ggggtcataa tgccgccgtc atacttcagt cgcgcgagga
    16381 tctcatcata accttcaccc tctttgttgc gaattggctg ggcgtagagc agcaaatcac
    16441 cctgatctaa cgccgtgcga atggtattca gcaatagcac ctgtttagtg gtttgcccgg
    16501 aaaccatctc ctctctgcta tccagcgcca gcacatgatg atgtgcgcag gattgctccg
    16561 ccagccagct taactgcccc aacaagggtt gcagggtttc ctgatttcca tcaaaatgcc
    16621 cccaggcagc accatagccc atatccagcc cggtattgtt ccagtgaatt tgccgactat
    16681 tcaggatgtt aaccatatgc tggagtcgcc cttccgtttc cggcccgctc agcactaaca
    16741 gcagttcact acccggcaat tgatacaact tttcgttttc ctgcatcaac ggcagcagcg
    16801 tacggtaaat tgagcggata cagtgaacgc gcattattaa gccgtaatga cgactcataa
    16861 actcaagatt atcaatgcgc aggcagcaaa aactcttgcc cgcctcttgc tccggcgctt
    16921 gctccaacgc acgaaagttg ggtagaagcg ttaacggatc ggtcagcgcc tgcaaatgcc
    16981 agcggcggtt aagccattca ctacgatgat aaatacgcac catatagagc aggcaaacac
    17041 taaaggaaat cagtactgcg agaataaatg ccagcgaata ttcggttagt accccttgca
    17101 aaaagttctg gttgtaattc agaaggcaaa gcgttgaaac agcccaggtg agatttaaaa
    17161 acggatagcg aagcttaccg acaccgaggg taaagatgat aaagaaaaca ggtaccaggt
    17221 aaccggcaat aaagtcattt tcatacggtg tgcacatcag aagtagcagc acgctaagag
    17281 ctgccagcca gcttaaggta aatgcgcgtt tctctttgct caacgacgga gcgatatccc
    17341 tacgccacaa tatctgtgca aagtggggac ttacaatcat gcgggtgaga tagtaaaaaa
    17401 gcatgttgta aatcagcact gcggtgaaaa ggcttagcaa atcaacgacc gtgaaaatgg
    17461 catccgcatc gccgaaaaag gtagatatct ttagcggaaa atcaaagaaa tttcccacaa
    17521 gatacatgct gcatttgatg ccaatcggcg ccaccaaacc aagccagaac agacgttgcc
    17581 agacgtatcg gctggtcaat ccataacgcc aacgcgtccc caattgccag cgtaatattg
    17641 cacaggcaca caggacagca aacgcctggc tgccgatcat gacggcggtc tgtaaaaaag
    17701 tgagatgaag gttccacaga ttggtgcaaa acatcccgca taaaatggga acgacaccac
    17761 gccagccaaa gataaacaag attgacagca ttacgcacag tggcatccac gccagaaaga
    17821 tataattgga atggataaca gcaagtggcg aaattaaacg ggatagctga ataagtacca
    17881 cggtgagcgt aaacgctagt gtgaatatct tgatattttt tatcaggtta tgctccacaa
    17941 acatgagtac actactctca ctgatcgggg ttttcaggca cacaaataat aagtttattc
    18001 gcatcgcgaa gcaaacaggg gccagcctgt tgctgttttt tcagtcagtg atgcattcac
    18061 cacacggcac tgccaggatg tagactgtat tacaaaagcg gcaaaaggca gagacaaaaa
    18121 acccccgctt tgcagcgagg gtttgaaatt tggtggagct aagcgggatc gaaccgctga
    18181 cctcttgcat gccatgcaag cgctctccca gctgagctat agccccacga gtcgtttacg
    18241 taccaaattt gctgggtgca aaatttggtg gagctaagcg ggatcgaacc gctgacctct
    18301 tgcatgccat gcaagcgctc tcccagctga gctatagccc catcacgtaa agcttgtcga
    18361 gttgacgggc ggcatcatat gaattccgcc cgaatgtgtc aacggcaaat tgccacgtgt
    18421 agtttcaatc gctgaaaaat caggcaaatg aacaattttg gaaaaccgct cgcattcagt
    18481 agttattcat gtcacggttt cctgtaaagt ggtgttataa aatgaactac taatagaccc
    18541 acatacattc agggaattgt tatgttcaag gagaggatga cgccagatga acttgccaga
    18601 ctgaccggct atagccgcca gaccattaat aaatgggtac gcaaggaagg ctggacaacg
    18661 tcaccaaaac caggcgtcca gggtggcaaa gctcgactgg ttcacgtcaa tgaacaggtt
    18721 cgtgaatata ttcgcaatgc tgaacgtcca gaaggtcagg gagaagcgcc agccctttct
    18781 ggtgatgcgc cgcttgaagt gttgctggtg acactggcga aagagatgac gccagttgaa
    18841 caaaaacagt ttacatcctt gcttctgcgg gaagggatta tcggattgtt acaacgctta
    18901 gggattcgcg atagcaaata atatgaaaag attacgcaat aaaatgacca ccgaagaact
    18961 ggctgaatgc ctcggtgtgg ctaaacaaac tgttaaccgt tggatcagag aaaaaggctg
    19021 gaaaacggaa aaatttcccg gcgtgaaagg cggtcgtgcc agacttattc tggtcgaaac
    19081 gcaagtttgc gagtttattc agaacacgcc agccttccat aacacaccga tgcttctgga
    19141 agccgaagaa ccccttgccg aatacgcacc gggtattcgc actcctgctt atcggcaaat
    19201 catcagcgca atagacaaca tgactcacag tgagcaagaa aaagttgcgc aatttttgtc
    19261 acgcgaagga attcgtaatt ttcttgctcg tctcgacata gatgaatcag cataaataaa
    19321 aaacggcagg ggtatctctc ctgccgttta tcttttatgc gctaattact gctgattttc
    19381 gcgttcagca ataaaagcca gcgctttgtt gatacgctcg atactgcggg tcttaccgat
    19441 tgcgtgaacg gtaacatcca gcgccggaga ctgccccgca ccggttacgg cgacacgcag
    19501 cggcatacca actttaccca tacccacttc cagctcatcc gccgtcgcct gaatagcgtg
    19561 atgaacgttt tcagcggtcc agtcagtaat cgcagtcagt ttgtcacgaa ccacttccag
    19621 cggctggcgc gctaccggac gcagatgttt tttcgcggcg tcggcatcga actcagcaaa
    19681 atcttcgtag aaataacggc agctctgtgc catctctttc agcgtcttgc agcgttcgcc
    19741 cagcagtttc accagatcag ccagctgcgg gccgttacgg gtatcgatat tttcctgctc
    19801 aatgtgccac tgtaagtgag tagcaacata ctccggcggc aacgcgttaa tgtagtgatg
    19861 gttcagccac agcagcttgt cggtgttgaa cgcactggca gatttgctga cggcattcag
    19921 agtgaagtat ttaatcatct cttcacgagt gaagatttcc tgatcgccgt gggaccagcc
    19981 cagacgcacc agatagttca gcagtgcttc tggcagataa ccatcatcac gatactgcat
    20041 tacgctgacc gccccgtgac gtttggacag ttttttaccg tcatcgccgt tgatcataga
    20101 aacgtgcgcg taaaccggta ccggcgcttt cagagcctta agaatgttaa tctggcgcgg
    20161 tgtgttgttg atatggtctt cgccacggat aacgtgggtg atttccatat cccagtcatc
    20221 gacaaccaca cagaagttat aggttgggga accatcggta cggcggataa tcagatcatc
    20281 cagctcctgg ttgctgaact cgatcggacc acggatctga tcgtcaaaaa caacagaacc
    20341 ttcctgcggg ttagcaaaac gtacgacaca cggctcatca tcagcgtgat gctcatgact
    20401 gtggcggcag cgaccgtcat aacgcggctt ctcacctttc gccatttgct cttcgcgcag
    20461 cgcttccaag cgctctttag agcaatagca tttataagct gtgccctctt ccagcatctg
    20521 atcgatcacc gcgttgtagc gatcaaaacg tttggtctgg tagtacggac cttcatccca
    20581 ctccaggctc agccagttca tgccatccat aatggcttcg atagcttccg gcgtggaacg
    20641 ctcaagatcg gtgtcttcaa tacgcagcac aaactcaccg ccgtggttac gtgcaaaaag
    20701 ccaggagtaa agagcagtac gcgcgccgcc aacgtgcaga tagcctgttg ggcttggcgc
    20761 gaagcgagtt ttgattttca tgaaatggcc ttacgtttag aaagatgccg acaaccggca
    20821 aatcctggaa aaattgagtg ggcgatattc tatcactccc gcctgattcc tcaatgtagt
    20881 tcgggttgca accttacgca ttattgtttt cgttgacaaa ttgcgcaaca cggtctgttt
    20941 tgcgatcatc ttgcttaaat ttacgacgaa cgaacaattt ctttagaaaa tgcgttgact
    21001 cattttgaac tctccctata atgcgactcc acacagcggg ggtgattagc tcagctggga
    21061 gagcacctcc cttacaagga gggggtcggc ggttcgatcc cgtcatcacc caccaactac
    21121 tttatgtagt ctccgccgtg tagcaagaaa ttgagaattg ggtgattagc tcagctggga
    21181 gagcacctcc cttacaagga gggggtcggc ggttcgatcc cgtcatcacc caccactttc
    21241 tcgccagcta gatttcttgt aacaatgtga agtaccgaag tgggtgatta gctcagctgg
    21301 gagagcacct cccttacaag gagggggtcg gcggttcgat cccgtcatca cccaccagtt
    21361 cgggtcgtta gctcagttgg tagagcagtt gacttttaat caattggtcg caggttcgaa
    21421 tcctgcacga cccaccaatg taaaaaagcg ccctaaaggc gcttttttgc tatctgcaat
    21481 ttgcgaaatt gcctgatgcg cttcgcttat cagaccaact atgtccggca gttcccgtgc
    21541 ctctcatcta ctgcgccaac gccgctagca gtttaaccag cgcgggcgtc acctgctgcg
    21601 tttcataaac aatatataag tctgccggga tgcgttcttt gagcggacgg aatacgacac
    21661 cgggccagtt catctgcgcg tagctgtccg ctatcaatgt gataccaata cccatactga
    21721 ccatggcgag taccgtttgc ggttcattaa cttcgcgaat aacaaccggt gaaaatccca
    21781 cctgctggca aactcgctgc aaaaaatccc agtcagtgta cacgggcggc attgtaacga
    21841 aatactcgtc acgtagcgct tccagcggga cggtggaaaa tgatgagaga tgatgctctt
    21901 caggcatcgc caccagaaac gccgattcat gcaaccgtaa gctggtaaaa ccagtcggtg
    21961 gttctgtcgc cattcgccag atcccggcat caagttcgcg gcgttccagc aaggccattt
    22021 gcatcgcagg catcttttcg cgaaacagaa cttcaacgtt aggattttct ctaaggaatc
    22081 gccgcataac cgggcgcatc cgtccccaca ttgccgttcc cacaaccccg agttcaatcc
    22141 gccctgcttc tccccgacct atttgttcaa cccgagccaa tacattattt gcattcacca
    22201 gcaatcgacg cgattcttcc atcaagattt tgcccgcgtg tgtcagtacg acgctgcgcg
    22261 aatggcgaat aaaaagctgc gtgccgagtt gattttccag ctctttaata tgaatactga
    22321 gcggaggctg agacatattt aaacgcgctg ctgcgcgacc aaaatgcaac tcttccgcta
    22381 cggcaagaaa ataacggagc aacttaagat ctgttctata tacgcgttcc ataattaagt
    22441 gctccatata aaaaatatat cgtattaaat tatcatggca taaagtattt caccaccaga
    22501 tatccgacat acataaacac gatggataat atacccggca atacatttgg aacaggaata
    22561 ggtaatcgca cgattgtaaa caaagcacca atgccaaaac caacgccaga agaaaataaa
    22621 atatctttca ctaattaacc tttatcataa aagcagcccc aaggaacaga gctgcgaatc
    22681 cttttaatgt gtaactgaac gatgcgttat cttttcaggg tcatggttat atttaaaccc
    22741 aaagaaaaat atcactgcga ggaaaagagc ataccctgca aacatcagcc aaatggtatg
    22801 ccaatccttt acgccatcca ccgagaagta atccactgcc ataccgctca gaatcgagcc
    22861 aacccatgcg ccgacgccat ttaccatcgt cataaaaagg ccctgggcgc tggcacgaat
    22921 gctggaatca acttcctgtt cgacaaagac cgaaccagaa atattgaaga aatcgaatgc
    22981 acaaccgtaa acaatcatcg acagcaacag caaaataaac ccggttggtg atggatcgcc
    23041 ataggcaaag aagccaaagc gcagcgtcca cgccaccata ctcatcagca tgacggtttt
    23101 aataccgaaa cgcttcaaaa agaacggtat cgtcagaatg aaaccgactt cagccatctg
    23161 tgaaactgac agtaaaatgg acgggtattt caccacgaag ctatcagcaa actcaggatt
    23221 acgggcgaaa tcatgcagga atgggttgcc aaaaacgttg gtaatttgca gcaccgcgcc
    23281 cagcatcatg gcgaagagga aaaagatagc catgcgtgga tttttaaaca gcacgaaggc
    23341 atccagaccc agcttgctgg caagcgatgt agtcgctttt ttctccgcaa ccggaatctt
    23401 cggcaaagtc aacgcataag ccgacagcag caatgacgca ccggacgcga tatacagttg
    23461 cagactactc aattccagat gcagcaggct tactgcccac atggcgacaa tgaaccccac
    23521 cgtaccaaaa acgcgaatgg gcgggaaagc ggtcaccggg tcaagcccta cctgggcaag
    23581 acaggaataa gagacgctgt tcgataacgc aatagtcggc ataaacgcca tcgcattgac
    23641 taacatcacc caaaacatca tatccggatc agttacggat gccgcataaa aaagtacgcc
    23701 cgcacacacc aggtgacaca gcatgtatgc acgttctgca cgcagccatt tgtctgcgat
    23761 gatccccatt ataccaggca taataatcgc ggcgatccct ttggaactgt aaaccatgcc
    23821 aacattagcg ccggtgaaat gaagggtatt aatcatgtaa gaaccgaggg taaccagcca
    23881 gctcccccag ataaaatatt gcaaaaagga cattaccttt aagcgcaaag cgatgctcat
    23941 aatcgtttcc ttgtcgcatc atatggcctc actcagtaag gccgtttatt gttgttttac
    24001 tcaggcaatt ttgcgcagaa agccgcaaat aagattaata aagttttgct ttgagagttc
    24061 cgctgctgcc agcgtttggg catgagaaag cttcacgtca ctcagacctt ccgccatgtt
    24121 ggtaatcgca gagaccgcaa cgactttaag ttcgcaatgg cgagctgaaa taacctcagg
    24181 caccacagac ataccaacaa catccccacc aataatttgc atcatgcgaa tttccgccgc
    24241 agtctcgaaa ttcggccccg gatacgagac gaacacgccc tccgtcagag ggaacccctc
    24301 ttctttcgcc actttttgta acagtgcgcg gtattccgca tcgtaggcat tcgccagcga
    24361 gaagaagcgc tcaccaaaac gttcatcatt aagacccacc atcggcgttc ccggcatggt
    24421 gttgatgtga tctttcaatg cgaccagact gccggccccc acttcaggac gcagtgagcc
    24481 agccgcattg gtgcagaaca gcaactcgca gcccagcaac ttaaaggtac ggattgcatc
    24541 cgtcatgatg gtcatcccac gaccttcgta gaaatgtccg cgacctttca tgcacgccac
    24601 tggcaccccc tggagatacc ccagcaccag ctcacccgca tgaccgtgta cggtacttac
    24661 cgggaaccca ggcagctttt cgtaggaaat tgcgaccgcg ttctcaatct gatcggccag
    24721 cgcgcccagc ccggaaccta aaataaaggc cactcgtggc gtgaaatcag gtttataagt
    24781 cttgataata tctacgcaat acagtgggtt atgagaaaaa tgagcctgat acatatatat
    24841 cctttttttg tagggtggaa tctaacgcct ttttataacg agattgcccc acctcatacc
    24901 aatacacttt tttcatgtat ccgatagtta ttcgatatcg atatatttat attttttcac
    24961 tattaatagg ctgaaaaaac tgtattggta ctctcctttc ccttgcatta acaatatgcc
    25021 gcttttaatc aaccttttta ttaagcaatc aactaataac gatcggcata gggaattatg
    25081 ctatgaaaaa acatcttttt actctgacac tctcttctgt attggcgata ccagcagtat
    25141 cccatgcaga atttaaaggc ggttttgctg atataggtat tcattatctt gactggacta
    25201 gcagaaccac tgagaaatcg tcgaccaaat cacacaaaga tgattttgga tatctggaac
    25261 tggaaggcgg cgctaacttt agttggggag aaatgtacgg gttcttcgac tgggaaaact
    25321 tttataatga tcgtcatgat aaaccgggta gcgagcagcg ttatacattt aaaaatacca
    25381 accgtattta cctgggtgac accggattta atctctactt gcatgcgtat ggcacctacg
    25441 gttctgcgaa tcgtgtgaat ttccatgacg atatgttcct ttacgggatc ggctacaatt
    25501 tcaccggcag cggttggtgg ttcaaaccgt tctttgcaaa acgctataca gaccaaacct
    25561 attataccgg cgataacggt tatgtcgccg gttgggttgc aggttacagc tttatgctgg
    25621 gcagtgagaa attcaccctc accaactgga acgagtacga gtttgatcgt gatgccacct
    25681 acgcggcagg taatggcggc aaagaaggtc tgaacggtgc cgtcgcactt tggtggaatg
    25741 caacttcaca catcaccaca gggattcagt atcgttatgc ggatgacaaa ctgggcgaag
    25801 atttttacca ggatgcgata atctactcca tcaaatttaa tttctgattg attactgccg
    25861 gagctcccgg cagtatgaat tcactctata tctgatacaa cggatcccct tcccgcccag
    25921 ccattagcgc attgccctgg tctcgtacta cgtcccataa cgcttgtgcg gcggtggaaa
    25981 gtgaacggtt cttcctgcgc accagcatca actggcgttc aaccactggc gtaatgcgct
    26041 ttaccaccag tgggctacct tcaggcaatg gtagcgccag tgcgggaaga atactgatgc
    26101 cgatgcctgc ggctaccatc gggaacagcg tcgccggatg accaatctcc tgcacaatat
    26161 tcgcctgaat accgttgcgc gccagcgctg cgtcaatcag cggtcggctg cctgacgcgt
    26221 aatcctgcaa caccaatttc gctccctgta atgcctgcca gggtacataa tcttccacgg
    26281 ccaaagcgct atcgcgatgg caaagcagaa agaaaggctc ggaaagaatc gcttcacatt
    26341 gcagatcgcc cactgggcct ggatcgatga cgatgccaaa atcgacatct ccctggcgaa
    26401 tactttccat cacccactgc tgcggacgat cgtgcaggac aaactgaata tctggatagc
    26461 ggcgatggct ttcagcaatg cattgcggaa taagatgcgc ggaaatggtc tggctggcag
    26521 cgacccgaac ttttccgctc agttgttgcc ccatacgccc ggtatcgcgc aacgtgctgt
    26581 taagttcatc cagcagtcgc tcaagacgca aagccaactg ctgccctgca tctgtaagca
    26641 ccacttcacg cgtggttctg tccagcaggc gaacaccagt atgattttcc agctccttca
    26701 cactgtgact cactgccgac tggctcaggc cgatacgctc tcctgcacga ctaaaacttt
    26761 tctcctgcgc tactgtgacg aaaaccttta attgctttaa agaataattc atctgttttt
    26821 ttcatgaatg gatgcaataa atcaatttta tttctcaaac tggaagaagc acaatagaac
    26881 catcgatcat ctggagtctt tatgaaactt tttcgtatcc ccgatccttt caccttaacc
    26941 ctgatcacag tggtgttgct ggcctctttc tttccggcca gaggcgattt cgtccccttc
    27001 tttgaaaatc tgaccaccgc agcgatagcc ctgctgtttt ttatgcacgg cgcgaagttg
    27061 tcgcgtgagg cgattattgc tggcggtggc cactggcgac tgcatttgtg ggtaatgtgc
    27121 agcaccttcg tgctgtttcc gattctgggc gtgctgtttg cctggtggaa accggtaaat
    27181 gtcgacccga tgctctactc cggttttctc tacttgtgca ttctccccgc gaccgtgcag
    27241 tctgcaatcg ccttcacgtc aatggcgggc ggtaacgttg cggcggcggt ttgttctgcg
    27301 tcagcatcca gcctgctggg gattttcctt tcaccattgc tggttggtct ggtgatgaat
    27361 gttcacggtg cagggggcag ccttgagcag gtcggtaaaa ttatgctgca actgttgctg
    27421 ccatttgtgt tggggcatct ttcccggccg tggattggtg actgggtgtc gcgtaataaa
    27481 aaatggattg cgaaaactga ccagacgtcc attctgttgg tggtttatac ggcattcagc
    27541 gaagccgtcg ttaacggtat ctggcacaaa gttggctggg gatcattgct gtttatcgtg
    27601 gtggtcagtt gcgttcttct ggctatcgtg attgtagtta acgtctttat ggcacgccga
    27661 ctgggcttca ataaggcaga tgaaattact atcgtctttt gtggttcgaa aaagagtctg
    27721 gcaaatggca tcccgatggc aaacattctg ttccccacat cggtgatcgg tatgatggtg
    27781 ctgcccctga tgattttcca tcagatccaa ttgatggtct gtgcggtgct ggcgcgtcga
    27841 tacaaacgcc agaccgaaca gttacaggcg cagcaggaaa gcagcgccga taaagcttaa
    27901 agcggacgct tcaggggctg gaccagttgc gtcagcccct cgtttttaat cagcagcgtg
    27961 atttgcatta gctcaccgag cttccctgca gggaactcat ctttgcgggc aaaccacagc
    28021 agatactcct ccggcaggtc gattaagcga tgccctttgt atttgccaaa cggcattatc
    28081 gtattggcta tttcaatcag ctgctctttt tccatctcag ctacccagca aacgcagcat
    28141 ttccgtttcg tcgatgactt caatgcccag ttcctgcgcc ttcgccagtt tagatcctgc
    28201 agcttcaccc gctatcacca gatcggtttt cttcgacacg ctgcccgcga ctttcgcccc
    28261 cagttcgacc agtcgagctt tagcgtcatc acgcgacatc tggcttaagc tgcccgtaag
    28321 caccacggtt ttaccagcaa acggactgtc aatctcttcc gcattgataa cgatcggctc
    28381 aggccagtga acaccttccg ccaacagctc gctgatgaca ttgcggttgc tttcttcggc
    28441 aaagaagttg tgaacgtggg aggcaacgac aattccaaca tcaggcacct tttgcagctc
    28501 ttcaatcgac gcggcttcca gcgcttccag cgtgccgaaa tatgccgcca gacctgctgc
    28561 ggtagcctcg ccgacttcac ggatgccaag tgcatagagg aagcgggcaa aggtggtttc
    28621 tttcgctttt tccagcgcgt taaccacgtt ttgtgccgat ttcggcccca tacgctccag
    28681 tccggtcagt ttgcctgcgg tgagtttgaa cagatccgcc ggagtgtgga catattcttt
    28741 ttcaaccagt tgatcgatga ttttgtcgcc cattccgtca acatccatcg cacggcggga
    28801 aacaaagtgt ttcagagact ctttacgctg cgcaccgcaa atcaggccac cggtacagcg
    28861 ggcaaccgct tcaccttcca cacgctcgac gtcagaacca cataccggac aatgcgtcgg
    28921 gaatacaacc tcacgggtat cttccgggcg ttcagaaagc acgacgttaa ccacctgcgg
    28981 gatcacatcg cctgcgcggc gaatcaccac tttatcgcca atgcgtaaac caagacgttc
    29041 gatttcgtca gcattatgca gtgtagcgtt actcaccaac acgccagcaa catggacagg
    29101 ttccagacgc gcaacaggcg taatcgcgcc agtacgccca acctgaaact cgacgtcacg
    29161 cacaaaggtc atctgttcct gcgccgggaa tttaaacgct accgcccagc gcggggcacg
    29221 cgcgacaaag ccaagctgct cctgctgtgc cagtgagttg accttaatca ccacgccgtc
    29281 gatatcaaag cccagcgtcg gacggtcttc ttctactttg tggtagaacg ccagcacttc
    29341 ttccgccgat tcacaaagcg ttacccgatc gctgaccggc aaaccccact ttttaaattg
    29401 cagtaaacgg ccaagatgag tatccggcag ctcgccacct tccagaacac cgacgccgta
    29461 gcagaagaaa gtgagcggtc gcttcgctgt aatacgcgga tcaagctgac gcagtgaacc
    29521 agctgccgca ttacgtgggt tagcaaacac tttcccgccc gtgcgtcgcg catcttcgtt
    29581 aatcttttcg aaccccgcct gcggcaggaa cacttcacca cgcacttcca gacgcgccgg
    29641 gatattctca ccgtgtagct tcagcggaat ggcgcgaata gtacgcacat tagacgtgat
    29701 atcttccccg gtggtgccat cgccacgagt cgcggcactg actaaaacgc cattttcata
    29761 cagaatactg acggcaagac catccagctt cagctcacag caccaggtga ctttctcgtt
    29821 gcttttcaga cggtcctgca ctcgtttgtt gaaggcaaga aagctttctt catcaaaaac
    29881 gttatccagc gacagcattg gcacttcatg gcggatctgg ctgaaagccg ccagcggcgc
    29941 agcacctaca cgttgggtag gcgaatcagg cgtaatcagt tctggatgtt tggtttccag
    30001 ctcgcgcagt tcgcgcatca gcctgtcgta ttcagcgtcg ggaatttccg gcgcatccat
    30061 cacatgataa agatattcat gatggcgaag cgtcgttcgc agttctgtca gttgttgttc
    30121 gattgattcc atatcgcacc atcaatgcta aaaacccccg acaagcgggg gttcgaagag
    30181 gagttaattt gccttaagtg tatcagacgt tggcgtcttt gacttcgcgg atgatgtcct
    30241 ggtactcgcg caatttctgc ggagtcatca tacggcgctg atcgtcaagc actacaccgc
    30301 ccacttcatc ggcaatatgc tgcgcagatt gcagcatcag cttaaagttc tgcagctcgt
    30361 caccgtaaga cggtacctgc ataaagatag tgacacccgg agtagtgaaa tccttcattt
    30421 caggatcaaa ggttcccggt ttcaccatat tcgccaggct gaataacgcc gggccgctgc
    30481 catccgggct aagatgacga tggtaaatgt tcatatcgcc aaaaatgaag cacgcttgtt
    30541 gaatgctgtt aagaagcagt tcaccgttta actcgctacc gtgatgcgcc gcgacgttca
    30601 tgataatcac cgcctctttg cgcttcggtt tatccgtaac tggagccggt tccgctacag
    30661 gctcaggctg tggtgccgct acgggttctg caggctggaa agcctgttgt gccggttgcg
    30721 gtgctgaatg cactggctgc ggcgctgacg cgacctgtgg cgaaacaggc tgctgcaacg
    30781 gctgttcagg ctgcggctga taggcaggct gctgcacagg ttgctgcacc ggctgcgttg
    30841 gacgcggagc atgttgcggc ggtacctgcg cttcaggcgg ctgctggacc ggttgacgcg
    30901 gctgcgcaga cgcataaggc ggttggtact ggtgttgcgg cgacggacga gcagcctcat
    30961 gctccagggc gttagccggg gcatgattca cgcggtgaac acgaacctca ccaacgccct
    31021 catcatcttc gacatcctcg tcataagaat cgtcgtcacg ttttgacttc attcgtttta
    31081 atggccgatc gcggaacata gaagatcgtt ctttacggct ggtccagaaa ccatgtacca
    31141 gtaaagcgat tatggcgatc gcgccaacaa tgattaatat cagacgcaaa tcctgcatca
    31201 ttatattctc tgttgttcta acaccttgcc accacggcaa acatttactc actaagagta
    31261 tttgccgatt acctcaagtg caagtgcact attaactttc acagcacaaa gatagatgaa
    31321 atcgtgcttt ttgctgtttt ttcgaacata tcctaactgt ccattgcgca attacccggt
    31381 aaaatacgca gaattttcct gggattggtc aaaaggagct catcctgatt atggtttcat
    31441 cattcacatc tgccccacgc agcggttttt actattttgc gcaaggctgg aagctcgtct
    31501 tgcaacctgg gattcggcgt ttcgttattt taccgctgct ggtcaatatt ttgttgatgg
    31561 ggggcgcatt ctggtggctc tttacgcaac tcgatgtctg gatcccgaag ctcatgagtt
    31621 atgttccgga ctggttacaa tggctgagct atctgttgtg gcccctggct gtcatctctg
    31681 tgctgttagt gtttggctat ttcttctcaa cgattgctaa ctggattgct gctccgttca
    31741 acggtttatt ggctgaacaa ctggaagcaa gactgactgg cgctacaccg ccagataccg
    31801 gaattttcgg tatcatgaaa gatgtgccgc gaatcatgaa acgcgaatgg caaaaatttg
    31861 cctggtatct gccgcgcgca attgtattgc taattcttta cttcatccct ggcattgggc
    31921 aaaccgtcgc gccggtactg tggttcctgt ttagcgcctg gatgttagcc atccagtatt
    31981 gtgattaccc cttcgataac cacaaagtgc cgtttaaaga gatgcgcacc gccctgcgca
    32041 cacgcaaaat caccaatatg cagtttggtg ctttaaccag cctgtttacg atgatcccgc
    32101 tgcttaatct gttcattatg cccgttgccg tttgtggcgc gacggcgatg tgggtcgatt
    32161 gctatcgcga taaacacgcc atgtggcggt aacaatctac cggttatttt gtaaaccgtt
    32221 tgtgtgaaac aggggtggct tatgccgccc cttattccat cttgcatgtc attatttccc
    32281 ttctgcatat agatatgcta aatccttact tccgcatatt ctctgagcgg gtatgctacc
    32341 tgttgtatcc caatttcata cagttaagga caggccatga gtaagatttt tgaagataac
    32401 tcgctgacta tcggtcacac gccgctggtt cgcctgaatc gcatcggtaa cggacgcatt
    32461 ctggcgaagg tggaatctcg taaccccagc ttcagcgtta agtgccgtat cggtgccaac
    32521 atgatttggg atgccgaaaa gcgcggcgtg ctgaaactag gcgttgaact ggttgaacca
    32581 accagcggta ataccgggat tgcactggcc tatgtagctg ccgctcgcgg ttacaaactt
    32641 accctgacca tgccagaaac catgagtatt gaacgccgca agctgctgaa agcgttaggt
    32701 gcaaacctgg tgctgaccga aggtgctaaa ggcatgaaag gcgcaatcca aaaagcagaa
    32761 gaaattgtcg ccagcaatcc agagaaatac ctgctgctgc aacaattcag caatccggca
    32821 aaccctgaaa ttcatgaaaa aaccaccggt ccggagatct gggaagatac cgacggtcag
    32881 gttgatgtat ttattgctgg cgttgggact ggcggtacgc tgactggcgt cagccgctac
    32941 attaaaggca ccaaaggcaa gaccgatctt atctctgtcg ccgttgagcc aaccgattct
    33001 ccagttatcg cccaggcgct ggcaggtgaa gagattaaac ctggtccgca taaaattcag
    33061 ggtattggcg ctggttttat cccggctaac cttgatctca agctggtcga caaagtcatt
    33121 ggcatcacca atgaagaagc catttctacc gcacgtcgtc tgatggaaga agaaggtatt
    33181 cttgctggta tctcttctgg agcagctgtt gctgcggcgt tgaaactaca agaagatgaa
    33241 agctttacca ataagaatat tgtggttatt ctgccgtcat cgggtgagcg ttatttaagc
    33301 actgcattgt ttgccgatct tttcactgag aaagaactgc aacagtaatg ccagcttgta
    33361 aaaaatgcgt aaaaaagcac ctttttaggt gcttttttgt ggcctgcttc aaactttcgc
    33421 ccctcctggc attgattcag cctgtcggaa ctggtattta accagactaa ttattttgat
    33481 gcgcgaaatt aatcgttaca ggaaaagcca aagctgaatc gattttatga tttggttcaa
    33541 ttcttccttt agcggcataa tgtttaatga cgtacgaaac gtcagcggtc aacacccgcc
    33601 agcaatggac tgtattacgc tcttcgtgcg tcgcgtctgt taaaaactgg cgctaacaat
    33661 acaggctaaa gtcgaaccgc caggctagac tttagttcca caacactaaa cctataagtt
    33721 ggggaaatac aatgttccag caagaagtta ccattaccgc tccgaacggt ctgcacaccc
    33781 gccctgctgc ccagtttgta aaagaagcta agggcttcac ttctgaaatt actgtgactt
    33841 ccaacggcaa aagcgccagc gcgaaaagcc tgtttaaact gcagactctg ggcctgactc
    33901 aaggtaccgt tgtgactatc tccgcagaag gcgaagacga gcagaaagcg gttgaacatc
    33961 tggttaaact gatggcggaa ctcgagtaat ttcccgggtt cttttaaaaa tcagtcacaa
    34021 gtaaggtagg gttatgattt caggcatttt agcatccccg ggtatcgctt tcggtaaagc
    34081 tctgcttctg aaagaagacg aaattgtcat tgaccggaaa aaaatttctg ccgaccaggt
    34141 tgatcaggaa gttgaacgtt ttctgagcgg tcgtgccaag gcatcagccc agctggaaac
    34201 gatcaaaacg aaagctggtg aaacgttcgg tgaagaaaaa gaagccatct ttgaagggca
    34261 tattatgctg ctcgaagatg aggagctgga gcaggaaatc atagccctga ttaaagataa
    34321 gcacatgaca gctgacgcag ctgctcatga agttatcgaa ggtcaggctt ctgccctgga
    34381 agagctggat gatgaatacc tgaaagaacg tgcggctgac gtacgtgata tcggtaagcg
    34441 cctgctgcgc aacatcctgg gcctgaagat tatcgacctg agcgccattc aggatgaagt
    34501 cattctggtt gccgctgacc tgacgccgtc cgaaaccgca cagctgaacc tgaagaaggt
    34561 gctgggtttc atcaccgacg cgggtggccg tacttcccac acctctatca tggcgcgttc
    34621 tctggaactg cctgctatcg tgggtaccgg tagcgtcacc tctcaggtga aaaatgacga
    34681 ctatctgatt ctggatgccg taaataatca ggtttacgtc aatccaacca acgaagttat
    34741 tgataaaatg cgcgctgttc aggagcaagt ggcttctgaa aaagcagagc ttgctaaact
    34801 gaaggatcta ccggctatta cgctggacgg tcaccaggta gaagtgtgcg ctaacattgg
    34861 tacggttcgt gacgttgaag gtgcagagcg taacggcgct gaaggcgttg gtctgtatcg
    34921 tactgagttc ctgttcatgg accgcgacgc gctgcccact gaagaagaac agtttgctgc
    34981 ttacaaagca gtggctgaag cgtgtggctc tcaggcggtt atcgttcgta ccatggacat
    35041 cggcggcgac aaagagctgc catacatgaa cttcccgaaa gaagagaacc cgttcctcgg
    35101 ctggcgcgct atccgtatcg cgatggatcg taaagagatc ctgcgcgatc agctccgcgc
    35161 tatcctgcgt gcctctgctt tcggtaaatt gcgcatcatg ttcccgatga tcatctctgt
    35221 tgaagaagtg cgtgcactgc gcaaagagat cgaaatctac aaacaggaac tgcgcgacga
    35281 aggtaaagcg tttgacgagt caattgaaat cggcgtaatg gtggaaacac cggctgccgc
    35341 aacaattgca cgtcatttag ccaaagaagt tgatttcttt agtatcggca ccaatgattt
    35401 aacgcagtac actctggcag ttgaccgtgg taatgatatg atttcacacc tttaccagcc
    35461 aatgtcaccg tccgtgctga acttgatcaa gcaagttatt gatgcttctc atgctgaagg
    35521 caaatggact ggcatgtgtg gtgagcttgc tggcgatgaa cgtgctacac ttctgttgct
    35581 ggggatgggt ctggacgaat tctctatgag cgccatttct atcccgcgca ttaagaagat
    35641 tatccgtaac acgaacttcg aagatgcgaa ggtgttagca gagcaggctc ttgctcaacc
    35701 gacaacggac gagttaatga cgctggttaa caagttcatt gaagaaaaaa caatctgcta
    35761 atccacgaga tgcggcccaa tttactgctt aggagaagat catgggtttg ttcgataaac
    35821 tgaaatctct ggtttccgac gacaagaagg ataccggaac tattgagatc attgctccgc
    35881 tctctggcga gatcgtcaat atcgaagacg tgccggatgt cgtttttgcg gaaaaaatcg
    35941 ttggtgatgg cattgctatc aaaccaacgg gtaacaaaat ggtcgcgcct gtagacggca
    36001 ccattggtaa aatctttgaa accaaccacg cgttctctat cgaatctgat agcggcgttg
    36061 agctgttcgt ccacttcggt atcgacaccg ttgaactgaa aggcgaaggc ttcaagcgta
    36121 ttgctgaaga aggtcagcgc gtgaaagttg gtgatactgt cattgaattt gatctgccgc
    36181 tgctggaaga gaaagccaag tcaaccctga ctccggttgt tatctccaac atggacgaaa
    36241 tcaaagaact gatcaaactg tccggtagcg taaccgtggg tgaaaccccg gttatccgca
    36301 tcaagaagta attcttgccg cagtgaaaaa tggcgcccat cggcgccatt tttttatgct
    36361 tcagccagag gcggcaaaat caattcatcg ctctcatgct gctgggtata gcgcatcact
    36421 tccagtacgc gcaaccccgc gcggtgcacc gcatcggtta acgccttccc tttcagcaat
    36481 ccactgataa gctgagcaca aaacaggtcg ccagtccctt tcaggtcagt tttaacccgg
    36541 gaatgggaaa tgacattcac gctgtcggca ctgaccacca ccacctgcat ctcctgattt
    36601 tcttcattgc cggaggcgct ggtaatcacc acccacttta atgtgtctga aagcagactt
    36661 tttgcggcag caatggcact gtcgagatcg cggcaatctt taccggtcag gatttccagc
    36721 tcaaagatat tgggggtaat tccctgtgcc agcggcagta aatattgtcg atacgcctcg
    36781 ggaaggtcag gtttgacata aattccgcta tcaatatcgc caatcaccgg atcgaccatg
    36841 atcaataggt caggatggtc tttgcgtagc gcagtcagcc actcggcaag gattttgatt
    36901 tgcgatgccg ttcccatata gcccgtggtt acagcacgaa gttggcgcag cgcatcacgc
    36961 tcctgaagcg cacgcaaata accgctaaac cattcatccg gaatcgcacc accgtagaaa
    37021 gtgtcataat gcggcgtatt gctcagcaat accgtcggca cggcaaagac attcaggccg
    37081 ttctgcttta tagcaggcac cgcaatgctg ttgcctacgc tgccgtacac tacctgcgac
    37141 tgcacggcga cgatatccgc ctgcagtgcc ctgctcttat cgttaaacaa caacaaacta
    37201 ctcatttaat tttttctcct tgccgatgat cctcatcgta ttccagccga aactttacct
    37261 gattctggca gtcaaatcgg ctattacaaa acaaggataa ggtaattcag tgaagaaaat
    37321 catttgtctg gtcgttacgc tgctaatgac actccccgct tatgcgaagt taacggccca
    37381 tgaagaagcc cgtatcaacg ccatgctgga gggattagca cagaaaaagg atttgatatt
    37441 tgtgcgcaac ggtgatgaac atacctgcga tgaagcggtt tctcatctgc gcctgaagct
    37501 gggcaatacc cgtaatcgca ttgatacagc cgagcagttt gttgacaagg tggcctcgtc
    37561 gtcatcgatt accgggaagc cgtatatcgt gaagatgccc ggtaagagcg atgagaacgc
    37621 acagcctttt ttacatgcgt taattgcgca gacggataaa acggtgcctg cgcaataatc
    37681 ttaaatcccc gccccctggc taaaatgctc ttctccaaac accccggtag aaaggtagcg
    37741 atcgccgcga tcgcagatga tcgccaccac caccgcgccg gggttagctt ttgccacccg
    37801 cagagctccg gcaaccgcgc cgccggagct aacgccacag aatattcctt cccgcactgc
    37861 cagttcgcgc atggtgtttt ccgcatcgcg ctgatgaata tccagcacct catccaccag
    37921 agaagcgttg aaaatccccg gcagatattc cgcaggccag cggcgaatgc cgggaatact
    37981 gctgccttct tccggctgca ggccaacaat ggtcaccggt ttggattgtt cgcgcataaa
    38041 gcgcgacacg ccggtaatgg tgccggttgt tcccatgctg gagacaaaat gggtaatacg
    38101 cccgccagtt tgctgccaga tttccggccc ggtagtggtg taatgggcgt aagggttatc
    38161 gggattattg aactgatcga gcaactttcc ttcgccgcga ttcgccatct ccagcgccag
    38221 atcgcgtgcg ccttccatgc cctgttcttt ggtgaccaga atcagttctg caccgtaggc
    38281 acgcatcgcc gcacggcgtt cctggctcat gttgtcgggc atcagcaatt tcatgcgata
    38341 gcctttaagc gccgcaatca ttgccagcgc aatgccggtg ttaccactgg tggcttcgat
    38401 cagcacatca cccggtttaa tctcgccgcg cttttccgcc tcgacaatca tcgaaagcgc
    38461 tgcacggtct tttaccgatc ccgccgggtt attgccttcc agttttaacc acacttcact
    38521 gccgttatcc ggcgccattc gctgcaactt caccagaggc gtattgccta ttgtttgttc
    38581 taatgtactc acgatctctg tccatacgtg gtgttgcctg atgtgacact tgcgcgtctt
    38641 atcaggccta caggttacaa aaccttgcca ttaaaaagcc cggatcgcgg gaagcgcctc
    38701 cgggcgttta acattcactc aacctatcag gcgctttgtg cgagagcaag ttcctcatcg
    38761 cgggtttcaa tacgctcatc gccgttatac agccgcgcat gttgcagacc aacgtataaa
    38821 cgatcgccac gctgcggggc atcgtcgcca tgcatcacga ccgtcagcgg ttcgttgtac
    38881 caccccagcg gctgcaccac taattgggtg tagtgacctt tcgggctggc ttccagtacc
    38941 tgtacgggta gcggcgaatc gaggctggta cggcggctga tatccacttc ccacgggcgc
    39001 aggaagagat ccaccggccc ctgatacgcg ggggtgtatc ccagcggcca gcgatgtgcg
    39061 ccaacgtgga actgcccgcc gcgaatggtt ccctgcaggc ggtttacctc gcccataaac
    39121 tccagcacaa aacgagtcgc cggttcacgc catacctgat ccggtgcgtc agcttgttca
    39181 atattgccct gactcatcac cactacacga tcggctactt cggtcgcctc ttcctggtcg
    39241 tgggtcacga acacgctggt gaatttcagt tcttcatgca gttgacgcag ccagcgacgc
    39301 agctctttac gcacctgcgc atccagcgcg ccaaacggtt catcaagcag caaaatttgc
    39361 ggttccacag ccagcgcgcg tgcaagcgcc acacgctgtt tctgaccgcc ggaaagctgc
    39421 gccggataac gatccgccag atgagcaagc tgcaccatct ccagcaattt tgtcactttc
    39481 gctttgatcg ccgccgcatt cgggcgttca cgccgtggca acaccgtcag gccaaaagcg
    39541 atattgtcaa acaccgtcat atggcgaaac agcgcgtaat gctggaacac gaaaccgact
    39601 ttacggtccc gcgcgtgcag acggctcacg tcggtaccgt gaaagcgaat atgcccactg
    39661 gtttgatgct ccagcccggc gataatgcgc agcagcgtag ttttcccgga accggacggc
    39721 ccaagcaacg cgaccatctg accagaagga atatccagtg agatatcgtt cagcacctgg
    39781 gtgcgaccaa aagacttctt aatattggca atctcaatgc tcatgatgtt cctcctgctg
    39841 cgcgcgtttt tcctgatttt ccaggcgcca ctgcaacata ctctttaaaa acagggtgat
    39901 aatcgccatc agcgtcaaca gcgcggcggc ggtaaaggag ccgacggtgt tgtagtcctg
    39961 ctccagcaat tcaatctgta acggcagcga cagagtttcg ccacgaatcg agccggaaac
    40021 caccgatacc gcgccaaact cgccaatcgc gcgggcgttg gtcagcacca cgccgtaaag
    40081 cagcgcccag cggatgttcg gcagcgtgac gcgacggaac atctgccagc cggacgcgcc
    40141 aagtaaaatc gccgcttcgt cttcctggct gccctgactt aacattaccg gcaccagctc
    40201 gcgcaccaca aacggacacg tcacgaagat ggtgaccagc accatacccg gccaggagaa
    40261 cataatttgc agattatgtt catccagcca gccgcccagc gggccgttag agccgtagaa
    40321 cagcagatac accagaccag caaccaccgg cgacacggca aacggaatgt ccagcagcgt
    40381 cagcaatagc tggcgtccgg ggaagttaaa gcgcgtcacc agccaggcca gcagaatacc
    40441 gaacaccagg tttaccggta cggcaatcaa cgcgatcatc accgtcagcc agatggcgtg
    40501 cagcatgtcc ggatcggcca gattttgtaa cactggcatc agccctttgc tgaacgcctg
    40561 tacgaagata taaatcatcg gtaccagcag gatgaacgcc gataccagca tcccgatgcc
    40621 aatcagaaac catttgcccc agttaatcgg gcgcgcgtaa taacgcttca attgtgtcac
    40681 ttccgccatt aatgacctac cacacgccga ccaaagcgac tttgcagagt gttaattgag
    40741 aacagcaaca gcagcgatgc cgcgaggatc accgaggcaa tcgcgctcgc cgccgggtaa
    40801 tcaaactcct gtaagcgaac aaagatcatc agcgacgtca cttctgtttt ccaggcgata
    40861 ttcccggcga taaaaatcac cgcgccaaac tcaccgaggc tgcgggtaaa cgacagcgcc
    40921 acacccgcca ccagcgccgg agatagttcc ggcagcacca ctttgcgaaa actctgccag
    40981 cgtgttgcgc cgagcgtttc tgccgcctct tcatattccg ggcctaactc ttccagcacc
    41041 ggctgcacgg tgcgcaccac aaacggaatg ctggtaaagg ccatcgccac cgcaatcccc
    41101 agccaggtat aggtgacttt gatatcaaac ttcgccagcc attcaccgta aaaaccgttc
    41161 acggaaaaga gcgaggccag cgttaaaccg gcgactgccg ttggcagcgc aaagggtaaa
    41221 tccatcagcg catcaagcag cgtgcgaccc ggaaagcgat agcgggttaa gatccacgcc
    41281 atcagcagac cgaaaacgcc gttaaaaatc gatgccacaa acgccgacag cagcgtcact
    41341 ttataggctg cgaccacctg cgggttggtg atcacctccc agtactgcgc ccagctcatc
    41401 tgggccagtt gcatcaccag cgcggagagc ggcagcagca aaatcaggca cacaaacagc
    41461 agactggtgc cgaggcttaa ggtaaagccc ggcagcacgc gtcttgaaga aactgcaaac
    41521 attacttacg ccccgccgct aacagcttgt ctaactcgcc gccgctggtg aagtgggttt
    41581 tcatcacttc cggccaggag ccaaatttat cttccacgcg gaacagctcg gtctgcggga
    41641 atttatcttt cagtttgccc atgacttccg ggttatttac gcggtagtaa tagtcggtga
    41701 tgatggtttg cgcctgcggg ctgtagagcc agttcaggta ggcttttgcc gctttttccg
    41761 tgccgttggc ctgtacgttt ttatccaccc acgccaccgg gaattccgcc agaatgttgg
    41821 ttttcggaat caccacttca aagccctgcg cttcatactg tttacggatg ttgttcactt
    41881 ccgactcgaa gctgatcagc acatcgccca ggccgcgctc ggcaaaagtg gtggtcgcgc
    41941 cacgaccgcc agtatcgaac acttcaacgt ttttcaggaa ctgggtcata aactgttcgg
    42001 ttttggcttt atcaccaccg tcggctttgt ccgctgcgcc ccatgccgca aggtaggtat
    42061 aacgcgcgtt acccgacgtt ttcgggttcg ggaaaatcag cttcacgtca gagcgtacca
    42121 ggtcgttcca gtcgtggata ttcttcgggt tgcccttacg caccaggaag cccatggtgg
    42181 agtagaacgg cgagctatta ttcggcaggc gcgactgcca gtccgctggg atcagcttgc
    42241 ctttatcgtg caggatttgt acgtcggtca cctggttata agtgacaacg tcggctttta
    42301 aaccctgcaa aattgccagc gcctgttttg atgatccggc atgagattgt ttaatcgtca
    42361 gtttgtcgcc accgttatct tttgcccatt gttgctcaaa cggcgggttc agggcggcaa
    42421 acagctcgcg ggagacgtca taagaactgt tcagcagttc cgttgcctgt acatggcccg
    42481 ccagtagcag agaagcaacc agcacgagtg agttcttttt cagtaagtta acggccattg
    42541 cgcaccctta taaatttaat gactttctaa tagccatcat atttataacg gacgtgaaag
    42601 gagtaacggt tttatatacc gtttggtgat ttggaagttg aaaagggaat aagacgaaat
    42661 cctccccaca aatgcaggga ggaaacagag gtgaatcaga tgccgacgct aaccgtctcc
    42721 ggcagtgtgc tgccgccatc aatcacattc tgtgtaccgg ttaaatagct ggattcatcc
    42781 gatgcgagga aggccgccag ttcgccgact tccagcggat cggcgaggcg acgcatcggg
    42841 attgctttcg ccatttcagt cagcaccgat tctggatctt ccgggttcga ctggcgggca
    42901 atgctttccg ccattggcgt gcgaacgtat ccagggcaga tggcgttaac gcgaatacca
    42961 gactgcgcgt actccaccgc cagcgattta gtcaggccaa caatcgccgc tttcgttaag
    43021 gcgtacgccg tttcgcccgg gtctgccacc atatcgccag tgactgaaga catcatcaca
    43081 atccggccat ctttgcgggc aatcatctcc ggcagcaccg ccttcgtgac gttccatacg
    43141 ccttttatat tgatatcaat atggaaatcg cgatcttcat cgctcatatc aaggaaactg
    43201 cccagacgac aaacgcctgc gttattcacc agaatatcaa tgcgcccttc tttttcctta
    43261 gcgcgcttaa tagctgcggc taccgacgcc gggtcacgca catcggcgac aaccgccgta
    43321 cagcgatgac cacggccaca cagttcgtcc gccagctttt cgatctcagg ggagatatcc
    43381 agcaagatta ggtttgcgcc atgacgcgca aaagttctgg caattccttc gccaattccc
    43441 tgcaatgcgc ccgtaatcag tgctgtcttg cccgtgagtt tacccatttt aatgctctcc
    43501 ttgttaatac agagcgttac actccattcc ctctgaaaat acaggataaa caaaatattg
    43561 ctgcggatca gctcactaat tcaggaaaat ctcaatttag tcgctgcttt atgcagcacc
    43621 tcaagcgtta cgtcagcgcg caatcatttt cagtgacatc acatgagtaa tattcggact
    43681 taatgattcc tttgtgtcta tcaaatcggg atatatgatc ccatttatct gcaattattt
    43741 gatcgcaaat attaaggtca ccaccgatgc aacttgaaaa gatgattacc gaaggctcga
    43801 acgccgcctc ggctgaaatt gaccgcgtat caacgctgga aatgtgccgg attatcaacg
    43861 atgaagataa aaccgtacca ctcgccgttg agcgcgtact gccggatatc gccgcggcga
    43921 tcgatgttat ccacacccag gtaagcggcg gcggccgtct gatttatctc ggtgcgggaa
    43981 catccggtcg tctggggatt ctggatgcca gcgaatgtcc gccgacctac ggcgtgaaac
    44041 cgggcctggt agttggtttg attgctggcg gcgaatatgc cattcagcac gcggtggaag
    44101 gcgcagaaga tagccgggaa ggtggtgtta atgatctgaa aaatattaat ttaaccgcgc
    44161 aggatgtggt ggttggtatt gcggccagcg gacgcacgcc ttatgtgatt gccgggctgg
    44221 aatacgcgcg ccaactcggc tgccgtacag tgggcatttc ctgtaatccg gggagtgccg
    44281 tttcatccac cgcagagttt gctattacgc cggtagttgg tgccgaagtt gttaccggtt
    44341 cttcgcgaat gaaagcaggc acggcgcaga aactggtgct caacatgctc tccaccgggc
    44401 tgatgattaa atccggcaaa gtgttcggca acctgatggt cgatgtggtc gccaccaacg
    44461 aaaaactgca tgtgcgccag gtcaatatcg tcaaaaacgc taccggatgt aatgccgaac
    44521 aagcggaagc ggcgttaatt gcttgcgagc gcagctgtaa aacggccatt gtgatggtac
    44581 tgaaaaatct cgatgccgat gaagctaaaa aatgcctgga tcaacacggc ggttttattc
    44641 gcaaggctct ggaaaaggaa taacccatgg ctaaagagat cagcagtgaa cttctgaaca
    44701 ccattcttac ccgggtcggc ggaccgggaa atatcgccag ttgtggtaac tgtatgacgc
    44761 gcctgcgtct gggtgtacat gacagttcat tggttgatcc cgatatcaaa acgctggaag
    44821 gcgtgaaggg cgtcattttg accagcgatc aggtgcaggt ggtttttggg ccaggcaaag
    44881 cgcatcgtgc cgcaaaagcc atgagcgagc tgctgggcga ggcaccggta caggatgccg
    44941 cagaaatcgc cgcccagaat aaacgccagt taaaagccag acaaacttcc ggcgtgcaac
    45001 aatttctcgc caaattcgcc accatcttca cgccgctgat ccccggtttt attgccgccg
    45061 gtctgctgct ggggatagcg acgttaattg ccacggtgat gcacgttccg gcagatgctc
    45121 agggcacgct acccgatgcg ctgaatttta tgaaggtgtt cagcaaaggc ctgttcacct
    45181 tcctggtcat tctggtgggc tataacgcgg cccaggcatt tggtggcacg ggcgtaaatg
    45241 gcgcaattat cgctgcgctc tttttgcttg gttataaccc aactgcaacc acgggttact
    45301 acgccggatt tcacgatttc tttggtctgc ccatcgatcc gcgcggcaat attatcggcg
    45361 tgttgattgc cgcatgggcc tgcgcccgca tcgaatgcat ggtgcgccgc tttatgccgg
    45421 acgacctcga catgctgttg acctcattaa tcaccctgct gatcaccgct acgcttgcgt
    45481 acctgatcat tatgccgctg ggcggctggc tgtttgaagg catgtcgtgg ctgtttatgc
    45541 acctgaacag taatccgctc ggttgtgcgg ttttagccgg gctgttcctg atcgccgtgg
    45601 tatttggcgt gcatcagggc tttattcctg tttatctggc gttaatggac agccagggat
    45661 tcaacagctt atttcccatc ctttcaatgg caggcgcagg ccaggtgggc gcggcgctgg
    45721 cgctctactg gcgggcgcaa ccgcacagtg cattacgcag tcaggtccgc ggggcgatta
    45781 ttcccggtct gcttggcgtt ggcgaaccgc tgatttacgg tgtcaccctg ccccgcatga
    45841 aaccgtttgt taccgcctgt ttaggcggcg cggcgggcgg tttgtttatc gggctgatag
    45901 cctggtgggg tctgccgatg ggcttaaaca gcgcctttgg gccgtctggt ctggtggcgc
    45961 tgccgctgat gacttccgcg caaggcatcc ttccggcaat ggcggtttac gctggcggta
    46021 ttctggtggc atgggttagt gggtttattt tcaccacact ctttggctgc cgtaacgtca
    46081 atctggactg attatgaaac ggacaatgct ctatctttct ctgctggcta ttagctgtag
    46141 cgtcagcgcc gcaaaatacc ctgttcaggc agaaagtagt tgatcaagtg tatgcggcgt
    46201 taaagcagta ataaaaaggc cagtcagttg tgttactggc tggcctttaa tcaatgaatc
    46261 agaaacgctt acagcgccat caacttgtcc agcgacggcg cgaagtaata gccgccagtg
    46321 accggtttgg tgaaacgcaa catcgcatca cgcttaccat cggtatcgcc aaacatgctc
    46381 agcagttgct gctcaatgtt atgcagacgc gcgcagtagg cgcagaagta cagaccgtga
    46441 gtgccactgg cggtgccgta cggcaggctc tgacgaacaa tcttcagccc tttgccatct
    46501 tctttcagat caacgcgggt caagtgagag gtttccggac gttcgtcgcc gtcaatctct
    46561 tcgttggcct ctttggtgcg cccaatcatc atctcttgat cgtgaacgct catccgattg
    46621 agctgcttca ggttgtgctc ccaacgctgc acaaacacat agctgccgcc cgcatccacg
    46681 ccgtctttga taacagccac ttcgcggcgc gtctcttcac ccgccgggtt ttctgtaccg
    46741 tcaacaaagc cgctcaggtc acgctcttca acccaacgga agccatggat ctcttctttc
    46801 acttcaatgc agtaaccaaa ggcttccatc gccgcctgag cgacagagaa gttcacgtcg
    46861 tgacgcagag aaagaatgtg gatcaacaca tcgaactggg tcgtcggtgc aagaccttta
    46921 ccgtagcccg gaaaatcttt cagctcttca gccccaacgc cgccgctcag agtgcgccag
    46981 gtattgttac caaaggcaac caccgcacca agatgcgcgt ccgggaattt cgcttcaaaa
    47041 gttgccagtt tgtcggcaaa tgttttactg gccgcacgca gggcgtcaac ttcccctttc
    47101 acgttggctt cgatccaaat cgccgcgcgg caatgttctg gcaaaatgcc actctgaacc
    47161 tgagacattg ttcctcctga aaataataat gccacgcaat ccgtggcatt gttgggcgct
    47221 attttacgtg ttttttcgcg gcggcggttt gttcagacgc aaattaacgc cgccagataa
    47281 ttttactgac tttccagttt ttcagggtat cgtccgaagg cattaaccct tcaggaccac
    47341 tccattcccc gctgaactgg tagctaatat gttgactgcc ttcagcttta cattcgacgg
    47401 cacgattatc atcactgtcg gccttttggc aattgccaaa tgctttgctg taaaggtcac
    47461 taaacggtgt accgatttta acaccggtgt cagcaggaat atcgctatcc agcacatcaa
    47521 tgcggctgat cgtgccttga tcaccgttaa tcaccatcgc cacgttgtcg cctttcatca
    47581 cttcaaaaaa gcgcaccacg ttgccgttcg cggttttcat tccgctgcgc aggcgataat
    47641 cgccatcaag cgcatcagca atggcttgtt cttgcagtgg tgtggacgcc gttaattcac
    47701 ccacgccctg ctcgctcact ttggtggatg atccaaacca gttccacggg ttagcggccg
    47761 accagttaac tgacgacatc gtggaacagc cggtcagcat caacggcata gcgcataaca
    47821 ttaaacgcag cgatttcatg tcacttcctt tggttattca ataacgttgc ttggagtgca
    47881 aattcaccaa aaagtgccgt tattcttttt cttcattaaa acaagcgcgt aaacgtcgat
    47941 tggtcagcag ccagattaac gccacgatat ccgccactac cagcgccaga ccgataccgc
    48001 taacggattc accgttcagc cacagccacg gttgccaaca aagtaaaacc acttgcgcca
    48061 acagcaacag aaaatagagc acatgccaga tacggggaaa tgaagcccgc cgaccactca
    48121 gcaaaaatgc cagcaccgcc ggaataccgg gaatcaaccc cagccagaaa ttatcgtgat
    48181 cgggataaaa cagatttagc agcgcagtac cctgctcgcg cgacgctccg gcaatgacaa
    48241 acagcaccca ggttcgcgcc tgaagcaata gcacaagcca gaagagcaag ggtaaacgca
    48301 gacgaccgtg cgcatcataa tggacaggat gaaactcagt actcttcatc ttcaatcaaa
    48361 cgcttaccca gactcagcac gtcggcgtgt tcatatccca ggcgttcata cattccgagc
    48421 accatgtcgt tatcttccgg cacgttgatc tgaattttcg gacagccgcg agcaatcagc
    48481 tttttctcca accgattaag caacgcatta gcaatcccac gcccacgaaa ctcgggatgc
    48541 acgccaagat aataagcaga cccgcgatgc ccgtcataac cgcccatcac cgttccgacc
    48601 acttcaccgt tcacctcagc gaccagaaac aaactgacgt catggttcat cttacgctca
    48661 atgtccattt ccggatcgtt ccacggacgc agcaagtcgc aacgctccca aagggtgatg
    48721 acctcttcga aatcttcctg gcgaaatacg cgtatctcca tggtattcgt tacctttttg
    48781 cgggttaaaa ggctgattat ggcgtgaacg gtcgaattag ccaatatctg acgaaaatcg
    48841 gttgaaaaag tggcataatg gggagttgtc aactattgaa atgaaaagta aaacaattct
    48901 caacagcaaa ccgtcgtaac ggattacgcg atacgatata acatctggaa ctttattatt
    48961 acaactcagg ccgtatgagc acttttaaac cactaaaaac actcacttcg cgccgccagg
    49021 tgctgaaagc cggtttggct gccctgacgt tgtcaggaat gtcgcaagcc atcgccaaag
    49081 aagaaccgtt aaaaaccagt aacggacaca gcaagccgaa agcaaaaaaa agcggcggca
    49141 gacgtgtcgt tgttctcgat ccaggccacg gcgggattga taccggagcg atcggacgca
    49201 acggttcgaa agaaaaacat gtggtgctgg caattgccaa gaatgtccgt tccatattgc
    49261 gtaatcatgg cattgatgcg cgtttaacgc gttctggcga tacgtttatc ccactgtacg
    49321 atcgcgttga aatcgcccat aaacatggcg cagatctgtt tatgtcaatt catgccgacg
    49381 gctttaccaa cccgaaagct gccggtgctt cggtatttgc cctctctaac cgcggtgcaa
    49441 gtagcgcaat ggcgaaatac ctgtctgaac gcgaaaaccg cgccgatgaa gttgccggta
    49501 aaaaagcgac tgacaaggat cacctattac agcaagtgct gtttgatctg gtgcaaaccg
    49561 atactattaa aaacagcctg acgcttggct cgcatattct gaagaagatt aagccggtgc
    49621 ataaactgca cagccgcaat accgaacaag ccgcgtttgt ggtgttgaaa tcgccgtcgg
    49681 ttccgtcggt gctggtggaa acctcgttta tcaccaaccc agaagaagag cggctgttag
    49741 gcactgcggc gtttcgccag aaaatcgcca cagcgattgc cgaaggcgtg atcagttatt
    49801 tccactggtt tgacaaccag aaagcacact cgagaaagcg gtaagtgatg aaacccgacg
    49861 cacacctggt taaacagttt ctgctcaacc ttcaggatac gatttgtcag cagttgagcg
    49921 ccgtcgatgg cgcagaattt gtcgaagata gctggcagcg cgaagctggc ggcggcgggc
    49981 gtagtcgggt tttgcgtaat ggtggtgttt tcgaacaggc aggcatcaac ttttcacatg
    50041 tccacggtga ggcgatgcct gcttccgcca ccgcccatcg tccggaactt gccgggcgca
    50101 gtttcgaggc gatgggcgtt tcactggtag tgcatccgca taatccgtat gttcccacca
    50161 gccacgcgaa tgtgcgcttt tttattgccg aaaaaccggg tgcagagccc gtctggtggt
    50221 ttggcggcgg cttcgattta acccctttct atggttttga agaagacgcc attcactggc
    50281 accgcaccgc ccgtgacctg tgcctgccat ttggcgaaga cgtttatccg cgttacaaaa
    50341 aatggtgcga cgaatacttc tacctcaaac atcgtaacga acagcgcggc attggcgggc
    50401 tgttctttga tgatctgaac acgccagatt tcgaccactg ttttgccttt atgcaggcgg
    50461 taggcaaagg ctacaccgac gcttatttgc caattgtaga gcgacgaaaa gcgatggcct
    50521 acggcgagcg cgagcgcaat tttcagctct accgtcgcgg tcgttatgtc gagttcaatc
    50581 tggtctggga tcgcggcact ctgtttggcc tgcaaactgg cgggcgcacc gagtcgatcc
    50641 tgatgtcaat gccgccactg gtacgctggg agtatgatta tcagccaaaa gatggcagtc
    50701 cagaagcggc gttaaatgag tttattaagg tcagggattg ggtgtaactc cctcaccccc
    50761 actcccgcat ccgctgatgc agcgtcagtg acggcttctc ggcaaacagt tgctgataat
    50821 ccgtggcaaa ttgcccaaga tgccagaatc cccactgcat ggcggcgtct tttaccgttg
    50881 tgctttgtga ccacggactt atgagttcgc ggcgcacggc gttcaggcga atacgtttca
    50941 gccacgcgtt cggaccaatg cccaaaatgg cgtgaaatgc gttttgtaat gtgcggcggc
    51001 tgacgtgcaa ttgattgcac aactccaaca ccgttaccgg ttcagacata ttttccagca
    51061 tatattcacg ggcgcgggaa agcaatcgac ggtaactctg atgactgatg ctttccgccg
    51121 tcatcatcgg ctgcgcttct tccagcatcg cccccatcgc cattagcaaa ttatccccca
    51181 gcacttttcg cactgcaggc tgatggagat tttccgggtt ctcgcaaaac gtcgccagcg
    51241 cctgttggac aaagccccac agcgcggctt tatgctgctc tttcacttcc agcgccgact
    51301 ggctacgcag catatgtaat acccgatccg gattatgcaa aaagttagcc tgacgagtga
    51361 tgacatcttc agaaagcact acgccaagga tcgtgtaatc gtccggcgta cttaattcaa
    51421 actcggttcc accagggcgc gtcgcaattt ctgcgcttcc cagacattgc gaaccgataa
    51481 atccctgctc accgcgcgtc gccggaatgc caaaccagaa cgagttcggc cagaccaggc
    51541 acgactgacg cagcgccaga ccagtgtatt cacgaaaaac ctgaatatca tcgagtagaa
    51601 tttccgtaaa ttcaccatga aacttgcccg gatgcagctg atcgtaaatc tgctgccagg
    51661 cggtaattgt caaagcatgt tcatagacgt ccgttgtctg tcgttgatga acattatcca
    51721 cttcgacctt cggcgtgagc ttcaggtttt cgggtaaggg ttcatgataa agatggtgca
    51781 aattggctgt acgggtcttt ttcatgatgt taatgccggg tgttgtagga cacccggcac
    51841 ctccgacagg ttaatggggc ttgagacggt aacgactact gcgtttacgt aacgtcccgg
    51901 cagaaaagag ctgttcgagc gcatttctgg ctttttccag cggccagcca aagtgggcag
    51961 ccacttctcc tgccgtcatt ccctgacgta ctgatgttaa cagcgccaac aactcgtcag
    52021 cagattccgc gacgataact ggcgcgtcgg gttcctttac gggttgcttc ggctggcggt
    52081 taaagccagt aaccagccac tgggtgtcat cgtctggccg accgatctcc ttacggctga
    52141 tgacacgccc ggtacgcatc gcggcagcac aacctgcgtc cagcgccgca cgacacgccg
    52201 ccagatcgcc ttccaccacc agcgttaggc gaccagggtc aagcacttcg tgactgagca
    52261 gacgaacgtt agctgctttg agcatggcat cagctgcatc tatcgcggcg accatgccgt
    52321 ccacttccag caatcccagt gcattgatca tcggcaacct ccgttacgca cgctggattg
    52381 gattacgcgc gatttccagc actgcatcgg taaaggcgtt acaggctgct ttacacgcgg
    52441 cctggctacc ggttaaaaat gctgccgagt agttggtttc agacggtggc gggacatagg
    52501 tcaccagctg cacgtcggca gatttcaacg ctgcatcaat gccgtaggtc gcttccagcg
    52561 gcggtgccac cagatacgcc atcggatcgc caagcgtgat cccggcggtt gatgagagat
    52621 aagaaccggt acgcgaaact acatgtgcca ggaaggctgt
//
LOCUS       DAZFFE010000020        50655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFFE010000020 DAZFFE010000000
VERSION     DAZFFE010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.020"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            75..1658
                     /gene="yegH"
                     /locus_tag="ACHJ69_001404"
     CDS             75..1658
                     /gene="yegH"
                     /locus_tag="ACHJ69_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO5014422.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQLLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(1726..3579)
                     /gene="asmA"
                     /locus_tag="ACHJ69_001405"
     CDS             complement(1726..3579)
                     /gene="asmA"
                     /locus_tag="ACHJ69_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO5014423.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLMA
                     AIEQINWQLQGADLPKQGIQGQGSFQVQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPMLALSGAGTLNLAEQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(3601..4182)
                     /gene="dcd"
                     /locus_tag="ACHJ69_001406"
     CDS             complement(3601..4182)
                     /gene="dcd"
                     /locus_tag="ACHJ69_001406"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO5014424.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4274..4915)
                     /gene="udk"
                     /locus_tag="ACHJ69_001407"
     CDS             complement(4274..4915)
                     /gene="udk"
                     /locus_tag="ACHJ69_001407"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO5014425.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5233..8550
                     /gene="dgcE"
                     /locus_tag="ACHJ69_001408"
     CDS             5233..8550
                     /gene="dgcE"
                     /locus_tag="ACHJ69_001408"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO5014426.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIIPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIA
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDGNNHQAAEVMSQADIACYASKNGGRGRVTIYEPQQAAAHNERAA
                     ISLDEQWRMIKENQLMMIAHGIASPRLPEASNLWLISLKLWSCESEIIDEQTFRRSFS
                     DPALSHALDQRVCHDFFRQAAKAVASKGLSIALPLSVAGLSSATLVNELLEQLENSPL
                     PARLLHLIIPAEAIFDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     NAQPLDLLVNRSYFAIN"
     gene            complement(8660..9508)
                     /gene="alkA"
                     /locus_tag="ACHJ69_001409"
     CDS             complement(8660..9508)
                     /gene="alkA"
                     /locus_tag="ACHJ69_001409"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO5014427.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTSRVAQLYGERLDDFPDYVCFPTPQR
                     LAVADLQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            9642..10994
                     /gene="yegD"
                     /locus_tag="ACHJ69_001410"
     CDS             9642..10994
                     /gene="yegD"
                     /locus_tag="ACHJ69_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5014428.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANCRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11007..12947)
                     /gene="yegI"
                     /locus_tag="ACHJ69_001411"
     CDS             complement(11007..12947)
                     /gene="yegI"
                     /locus_tag="ACHJ69_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO5014429.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTMPSFVGFERTENHDNFGLALLIFHILFGGR
                     HPYSGVPLISDAGNALEMDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDLLRQQLKKCTVSAMHVYPAHLADCPWCALDNQGVIY
                     FIDLSEEVITTGGDFVLARVWAMVMASVAPPALQLPLPDHFQPTGRLLPSGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILIPVLAAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRSVKQVRGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKMAAKRHRLESALTVGATELQRFRLQAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(12944..13705)
                     /gene="pphC"
                     /locus_tag="ACHJ69_001412"
     CDS             complement(12944..13705)
                     /gene="pphC"
                     /locus_tag="ACHJ69_001412"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFO5014430.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAIRDFACTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKLFLSSPAVNERTDDDKTLALAMWLP"
     gene            complement(13702..14361)
                     /gene="yegL"
                     /locus_tag="ACHJ69_001413"
     CDS             complement(13702..14361)
                     /gene="yegL"
                     /locus_tag="ACHJ69_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO5014431.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFTIG
                     VQGADMKTLAQISVRQPLSLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14579..14635)
                     /locus_tag="ACHJ69_001414"
     CDS             complement(14579..14635)
                     /locus_tag="ACHJ69_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5014432.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            complement(14907..14963)
                     /locus_tag="ACHJ69_001415"
     CDS             complement(14907..14963)
                     /locus_tag="ACHJ69_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5014433.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            15242..16489
                     /gene="mdtA"
                     /locus_tag="ACHJ69_001416"
     CDS             15242..16489
                     /gene="mdtA"
                     /locus_tag="ACHJ69_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO5014434.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSATPGATKQAQ
                     QSPAGGRRGMRAGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            16489..19611
                     /gene="mdtB"
                     /locus_tag="ACHJ69_001417"
     CDS             16489..19611
                     /gene="mdtB"
                     /locus_tag="ACHJ69_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO5014435.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSESVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGCGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            19612..22689
                     /gene="mdtC"
                     /locus_tag="ACHJ69_001418"
     CDS             19612..22689
                     /gene="mdtC"
                     /locus_tag="ACHJ69_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO5014436.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDDTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRARLPELQSTIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGAVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRKKLAKEPGANLFLMAVQDIRVGGRQANASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQEDNGAEMNLIYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQAVTE"
     gene            22690..24105
                     /gene="mdtD"
                     /locus_tag="ACHJ69_001419"
     CDS             22690..24105
                     /gene="mdtD"
                     /locus_tag="ACHJ69_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5014437.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQIGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGFSPLAIAGLVAVGVVALVLYLLHAQNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRWVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFVF
                     ARVPSDTHQNVAISRRKRSAQ"
     gene            24102..25505
                     /gene="baeS"
                     /locus_tag="ACHJ69_001420"
     CDS             24102..25505
                     /gene="baeS"
                     /locus_tag="ACHJ69_001420"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO5014438.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLITMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSLEHDNNEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPVPPDGTRRPIMVNGAEVGAVIASPVERLTRNTDINFD
                     RQQRQTSWLIVALSTLLAALATFPLARGLLAPVKRLVDGTHKLAAGDFTTRVAPTSED
                     ELGRLAEDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TLASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     LSITVELPLERDLQREV"
     gene            25502..26224
                     /gene="baeR"
                     /locus_tag="ACHJ69_001421"
     CDS             25502..26224
                     /gene="baeR"
                     /locus_tag="ACHJ69_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO5014439.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            26404..26736
                     /gene="yegP"
                     /locus_tag="ACHJ69_001422"
     CDS             26404..26736
                     /gene="yegP"
                     /locus_tag="ACHJ69_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO5014440.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            26883..28244
                     /gene="yegQ"
                     /locus_tag="ACHJ69_001423"
     CDS             26883..28244
                     /gene="yegQ"
                     /locus_tag="ACHJ69_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO5014441.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKN
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGELAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(28744..29061)
                     /gene="yegR"
                     /locus_tag="ACHJ69_001424"
     CDS             complement(28744..29061)
                     /gene="yegR"
                     /locus_tag="ACHJ69_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO5014442.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDATHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            29476..30375
                     /gene="yegS"
                     /locus_tag="ACHJ69_001425"
     CDS             29476..30375
                     /gene="yegS"
                     /locus_tag="ACHJ69_001425"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO5014443.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAVRFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDEIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQINKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEIIPTLVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(30457..31230)
                     /locus_tag="ACHJ69_001426"
     CDS             complement(30457..31230)
                     /locus_tag="ACHJ69_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5014444.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSGVDKLALQKTRVKLITV"
     gene            complement(31336..32376)
                     /gene="gatD"
                     /locus_tag="ACHJ69_001427"
     CDS             complement(31336..32376)
                     /gene="gatD"
                     /locus_tag="ACHJ69_001427"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO5014445.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLFPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRNNVFALPADMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSREMSAP
                     QIQGVLRDLRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(32424..33779)
                     /locus_tag="ACHJ69_001428"
     CDS             complement(32424..33779)
                     /locus_tag="ACHJ69_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO5014446.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIDLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYLGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIVIGAIYLTGIFMTWRRARGFIKQEKAVLA
                     E"
     gene            complement(33783..34067)
                     /gene="gatB"
                     /locus_tag="ACHJ69_001429"
     CDS             complement(33783..34067)
                     /gene="gatB"
                     /locus_tag="ACHJ69_001429"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO5014447.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(34098..34550)
                     /gene="gatA"
                     /locus_tag="ACHJ69_001430"
     CDS             complement(34098..34550)
                     /gene="gatA"
                     /locus_tag="ACHJ69_001430"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO5014448.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPEIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(34560..35822)
                     /gene="gatZ"
                     /locus_tag="ACHJ69_001431"
     CDS             complement(34560..35822)
                     /gene="gatZ"
                     /locus_tag="ACHJ69_001431"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO5014449.1"
                     /translation="MKTLIARHKVGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAGRAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITQVEDAANTLRTHQKAFIARGLAEALTRVIA
                     IVVQPGVEFDHSNIIHYQAQEAQALAQWIEKTKMVYEAHSTDYQTQTAYRELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMFDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGMEIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGSAE"
     gene            complement(35851..36705)
                     /gene="gatY"
                     /locus_tag="ACHJ69_001432"
     CDS             complement(35851..36705)
                     /gene="gatY"
                     /locus_tag="ACHJ69_001432"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO5014450.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLTIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPVQA
                     REFAEATGIDSLAVAIGTAHGMYARAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(36932..37984)
                     /gene="fbaB"
                     /locus_tag="ACHJ69_001433"
     CDS             complement(36932..37984)
                     /gene="fbaB"
                     /locus_tag="ACHJ69_001433"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO5014451.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            38241..39518
                     /gene="yegT"
                     /locus_tag="ACHJ69_001434"
     CDS             38241..39518
                     /gene="yegT"
                     /locus_tag="ACHJ69_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO5014452.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWAGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            39515..40519
                     /gene="yegU"
                     /locus_tag="ACHJ69_001435"
     CDS             39515..40519
                     /gene="yegU"
                     /locus_tag="ACHJ69_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO5014453.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILGWALRFDAFNKN
                     VLGPTSKIALNDIRDGKPVAELENNGVTNGAAMRISPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            40516..41481
                     /gene="yegV"
                     /locus_tag="ACHJ69_001436"
     CDS             40516..41481
                     /gene="yegV"
                     /locus_tag="ACHJ69_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO5014454.1"
                     /translation="MSSARLHTLLPELTPRQPVMVIGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEDLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(41455..42201)
                     /gene="yegW"
                     /locus_tag="ACHJ69_001437"
     CDS             complement(41455..42201)
                     /gene="yegW"
                     /locus_tag="ACHJ69_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5014455.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQSLEEEGVVTRSRGYGTQINNIFEYSLKEARGVS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(42253..43071)
                     /gene="yegX"
                     /locus_tag="ACHJ69_001438"
     CDS             complement(42253..43071)
                     /gene="yegX"
                     /locus_tag="ACHJ69_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO5014456.1"
                     /translation="MQLRITSRKKFTVLLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            complement(43136..43936)
                     /gene="thiD"
                     /locus_tag="ACHJ69_001439"
     CDS             complement(43136..43936)
                     /gene="thiD"
                     /locus_tag="ACHJ69_001439"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO5014457.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAQRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(43933..44721)
                     /gene="thiM"
                     /locus_tag="ACHJ69_001440"
     CDS             complement(43933..44721)
                     /gene="thiM"
                     /locus_tag="ACHJ69_001440"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO5014458.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMSAAVEQATRSQTPWTLDPVA
                     VGALDYRRRFCVELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVMVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENIASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(44756..44852)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(44944..45216)
                     /gene="rcnR"
                     /locus_tag="ACHJ69_001441"
     CDS             complement(44944..45216)
                     /gene="rcnR"
                     /locus_tag="ACHJ69_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO5014459.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            45337..46161
                     /gene="rcnA"
                     /locus_tag="ACHJ69_001442"
     CDS             45337..46161
                     /gene="rcnA"
                     /locus_tag="ACHJ69_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO5014460.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSLGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            46380..46718
                     /gene="rcnB"
                     /locus_tag="ACHJ69_001443"
     CDS             46380..46718
                     /gene="rcnB"
                     /locus_tag="ACHJ69_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO5014461.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(46800..47834)
                     /gene="yehA"
                     /locus_tag="ACHJ69_001444"
     CDS             complement(46800..47834)
                     /gene="yehA"
                     /locus_tag="ACHJ69_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO5014462.1"
                     /translation="MEIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLESIPVSQLPATGN
                     KTTINSKKGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGTPVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(47850..50319)
                     /gene="yehB"
                     /locus_tag="ACHJ69_001445"
                     /pseudo
     CDS             complement(47850..50319)
                     /gene="yehB"
                     /locus_tag="ACHJ69_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            complement(50335..50655)
                     /locus_tag="ACHJ69_001446"
                     /pseudo
     CDS             complement(50335..50655)
                     /locus_tag="ACHJ69_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial assembly protein"
BASE COUNT        12157 a        13286 c        12649 g        12563 t
ORIGIN      
        1 ttgcattttt gccccgattt atccatgatc gaattgtgac atttgtcata caacgaatag
       61 gttttgtact tactatggaa tggattgccg atccgtctat ctgggctggg ttaatcacgc
      121 tgattgtgat cgaactggtc ctcggcattg ataacctggt ctttattgcc atcctcgccg
      181 aaaaactacc gcctaagcag cgcgaccgcg cacgggttac cgggctcctg ctggcgatgt
      241 taatgcgcct gttactgctg gcgtcaatct cctggctggt caccctgact caaccgctgt
      301 tcagcttccg ctcgtttacc tttagcgccc gcgacttaat catgctgttt ggtggtttct
      361 tcctgttgtt caaagccacg atggagctga acgaacggct ggaagggaaa gacagcaata
      421 accccaccca acgcaaaggt gcgaagttct ggggggtggt gacacaaatt gtggtactgg
      481 acgccatctt ctcactcgac tcggtgatta ccgccgtcgg gatggtcgac catttactgg
      541 tcatgatggc cgccgtggtt atcgctatca gcctgatgtt gatggccagc aagccgttaa
      601 cccagttcgt taacagtcac ccgaccatcg ttattctctg cttaagcttc ctgttgatga
      661 ttggctttag cctggtggcg gaaggtttcg gcttcgtcat tccgaaaggc tacctgtacg
      721 ctgccattgg tttctcggtg atgatcgagg cgctcaatca gttggctatc tttaaccggc
      781 gacgttttct ttccgctaat cagacgctgc gccagcggac taccgaagcg gtaatgcgcc
      841 tgcttagcgg gcaaaaagaa gatgcggaac tggacgccga aaccgcgtcc atgctggtgg
      901 atcatggcaa ccagcagatc tttaatccgc aggaacggcg gatgattgaa cgggtactta
      961 atcttaacca gcgtaccgtc agcagcatta tgacgtcgcg ccacgatatt gagcatatcg
     1021 atctcaacgc gccggaggac gagatccgcc agttactgga gcgaaatcag catacgcggc
     1081 ttgtcgttac tgatggcgat gacgcagaag atttgctcgg tgttgttcac gttatcgacc
     1141 tgctacaaca gttactgcgc ggcgaaccgc tcaacctgcg ggtgttgatt cgtcagccgc
     1201 tggtgttccc ggaaaccttg ccgttgttac ctgccctgga gcagttccgt aatgcccgca
     1261 cacactttgc ttttgtggtg gatgagtttg gctcggtgga agggattgtg acattaagtg
     1321 acgtcactga aaccattgcc ggtaacttac cgaacgaagt ggaagagatc gacgcccgtc
     1381 atgatattca gaagaatgcg gacggttcct ggacggcgaa tggtcatatg ccgctggaag
     1441 atctggtgca atatgtgccg ttgccgctgg atgaaaaacg tgaatatcac accattgccg
     1501 ggctattgat ggaatatttg cagcgtattc caaaacctgg cgaagaagtt caggtggggg
     1561 attatttgct taaaacgttg caggtggaaa gccatcgcgt gcagaaggtg cagattattc
     1621 cgctgcgtaa ggatggcgag atggagtacg aggtgtgatg gcatgtaggc ctgatgcgac
     1681 gctttacgcg tcttatcagg cctacaggac agctcacctg gtaacctaca tcttctccag
     1741 caacttcttc acatctttgc cattgcggga atctttattc cgctcggccc agtcgttaag
     1801 acggcgtttt gcttcatcct gtagatgttt acgcagtaac tgatccactt gcaggctgta
     1861 attaagttgc tgccagttgc cataaacccg cagcggtact ggcgtttctt tgaggaaatc
     1921 aatcagtttg ctttctccgt tccagccacc cacgacccga atatcaaact gggtgtcgca
     1981 ggtttgctcc gccagattca acgtaccagc cccggaaagc gccagcatgg gcgattgacc
     2041 ttgcatgtcg tttaacgtca cgacgccatc cttcaacgtc aaatcggtgg taaagcggtc
     2101 aagacgcgtc acgttatcga agttttcagc ggccttcaca tcaccaccat tacgttctac
     2161 cgcttgctga atcatctgct ggaagttcat cccttccatg cgtgtgtcgg tcatttcgac
     2221 atgtgcctgt ccttgccagt tgtggcggaa tgcgtcggca tctatgtcag caccggagaa
     2281 atcaccagcc agtgacatct ttccggtcaa cgaaatcgga tagttaaacg ccttcagaat
     2341 ggtgccaatc tcaacgtttt ccagccgtgg ctggaagttt atccgcggat ttattgatgt
     2401 cgcgtccagc gtgcccggca gtgaaacctg tccaccgtta agtttgccct gcagttgagt
     2461 aatttccagc aaacccgact tgttggtcat ttgcgtggca acatctgtaa aattcattcc
     2521 gcgccagcgc acgttactgg cctgcaacaa aatatcagcc gtaaagcctt gcagtccctg
     2581 ataggccggt tcatcaatac gcgaagaaat gaccgggcgc ggcaacgttg attggctctg
     2641 cccttgctgc gcggcaccgt tctcaccgtt cgctgtttca ttaaggggga tgaggttgtc
     2701 aagattcagt tgcgggaatt gcagtctcag ctgccattcc ggtttctctg tcaacgtgac
     2761 ctgtgcttgc ccgctcagcg tactgtcatt ggcggtcaaa ctaatttggt taaatgaaag
     2821 gcgtttatgt gactcctgcc actggacctg gaaactcccc tgcccctgaa taccttgttt
     2881 tggtaaatcg gcaccctgca actgccagtt aatttgttca atagccgcca ttaaatcatg
     2941 cggataatca gaagcatcca ccgtaccgtt aagggatata gtgagatcgc gctgatcgcg
     3001 attaacccgc ccggagaact caaatgagcc acgatgttgg ggatcttgtt ccatttgcag
     3061 gcggatattg cggattgtca cctgctcgtc atcttcatgc tggaacacca gaacgctgtc
     3121 cgccacctta agactggata tatcaaacga ccatccgcga tcgtctgaca gatccggcaa
     3181 ggtattgtcg cgcggtgcaa ccggagcgtc ttcactgcgc acagcttccg tctgcggcgt
     3241 cagttggatc actgccccct ttagcatcac ctgcttaacg ctcagttgat gactcagtag
     3301 tggtaaaagc gccacgtcca gacgcatgtt gtcggcgcga accagtggct ggcttgcgcc
     3361 ctgggcggtg agagacattc gcccggagag gatactaagc tgcggccaga cgtgccaacg
     3421 cagtggcccg tcgagctgca attgataacc gctacgtgca gcaacttgct tgaccatata
     3481 gtcgcggaaa tcattcggat tcaccagcaa cactaacgca gataacccgg ccaccagcac
     3541 gaccaggagt atcatcagcg tcgtcagaaa tcgtctcatg gtatcctcaa tgggcctgaa
     3601 ttagtcttta tcgattcggc tggctaccgc gccctgctgg ttgcgatatt tcgcatcttc
     3661 acggcggttg taaggtcgcg ccgccgggcc ggagagcggc tcaaagctca gcgcaccaat
     3721 taacatgccc ggacgcagcg ccagcggcag cttaccggag ttgtaaaact ccagcacaat
     3781 gcagccagac cagcccggat cgatacggtg cgcggtgacg tgcaccatca gccccagacg
     3841 cgccagtgaa gaacgcccgt ccagccagcc caccagatcg gctggcagcg tcaccgactc
     3901 cagcgtcacc gccagcgcca gctctcctgg gtgaagataa aacgcctcgc cctcgtcgag
     3961 cacaatttca tcgctcatca cgcggtcaag cgcggcgctc acttcatctt tgggaccgct
     4021 cagatcgata aacgctgccg tgtgaccacg gaaggtacga aatttattgc ccaggcgcac
     4081 atccaccgtc gcgccgttaa tacgctccac tggcggacgt gggttgatcg acaaacggcc
     4141 ttcatcaagc caggcttcaa tatctcggtc acacagacgc atggcatttt ctcctttcga
     4201 gcatcactcc cttaacgcca attacgtcaa gggcatacca ggttatcact gaacggtaca
     4261 caatttatca agcttattca aagaactgac ttattttcgc tttcaatata tcgatcgcga
     4321 tgcggttttt cccgccgcgc ggcacgataa tgtccgcata ttgtttagaa ggctcaatga
     4381 attgcagaaa catcgggcgc acggtttttt gatattgcgc catcactgaa tccattgaac
     4441 gcccacgctc gttaacgtca cgcttgatgc ggcgcatcag gcagatatcc agcggggtat
     4501 caacgaaaat ggagaagtta agttcgtcac gtaaacgcgc atccgtcagc aacaaaatgc
     4561 cttcgagaat gatgaccttc ttcggctcaa ccgtcaccgt ttctttcata cgcgtatgtt
     4621 caacatagct gtaaaccggc aggtcaattg ccgagccgcg tttcaacgct tgtaaatgct
     4681 caagcagcag gctgtgatcc atcgcgctgg gatggtcgta gttggtctta acgcgttctt
     4741 ccatcgacag atggctttga tctttgtaat agcagtcttc gggaattacg ccgatgtgtt
     4801 catcaccgac ttgctcacgt aattcacgat aaagggtact ggcaataaga ctcttgccgg
     4861 aagccgatgc gccagcgata ccgataatga cgcactgatg agactgatca gtcatatatt
     4921 tagcgacctg attaacctgg atgttaggaa gggggcgacg aagcgccaaa cgcggcaatt
     4981 atagggattt catccgcctg ataccagtcg aatagcgttg ccgcgcgctc agagttaatt
     5041 gttgacgaag aattcccggt ggcaaattac gttgatcagt tttatacaag gtaaaaaatg
     5101 ttatacgcag ttgcgcaaat tatccgcctt tacgtcactt tatgagcaat tcgcatataa
     5161 aatgtaaaac ttttgtacta gcataaacac agaaacgaat actggcgacc tggccttgcg
     5221 gataaagcgg taatgagcaa acaatcacag catgtattaa ttgccctgcc ccacccgctg
     5281 cttcacctgg tcagtttagg tttagtctcg tttatcttca cccttttctc acttgagctt
     5341 tcgcagtttg gcacccaact cgccccactg tggttcccga cctccatcat gatggtggcg
     5401 ttttatcgcc atgccgggcg catgtggccg ggaattgcgc tgagctgctc gctgggaaat
     5461 atcgccgcat ccatcctgct tttttccacc agctcgctga acatgacctg gacgaccatc
     5521 aatattgttg aagccgtggt cggggcagtg ctgctacgta aattgctgcc gtggtataac
     5581 cccttgcaaa atctggctga ctggctgcgt ctggcactcg gcagcgccat tattccgcct
     5641 ctgttggggg gtgttctggt tgtcctgctg acgcccggag acgatcctct cagggcattt
     5701 ttgatatggg tactgtcaga atccatcggc gctctggcac tggtgccgct gggattgtta
     5761 tttaaaccac actatctact gcgccatcgc aacccaaggt tgctttttga gtcgctgctc
     5821 acgttagcca tcacgctgac gttaagctgg ctttcgatgc tgtatctgcc gtggcctttt
     5881 actttcatta ttgtgctgtt gatgtggagc gccgtgcgcc tgccacgaat ggaagccttt
     5941 ttgatcttcc ttaccacagt gatgatggtg tcgctgatga tggccgcgga tccctccctg
     6001 cttgctacgc cgcgtacgta cctgatgagc catatgccgt ggctaccgtt tttgctgatc
     6061 ctgctgcccg ccaacatcat gacgatggtg atgtatgcct ttcgtgcgga acgcaaacac
     6121 atttccgaaa gcgaaacccg ctttcggaac gcgatggaat attccgctat cggcatggcg
     6181 ttagtgggca ccgagggaca atggctgcaa tccaacaaag cgctctgcca gtttctcggt
     6241 tacagtcagg aagagctgcg cggactcacc tttcagcaac tgacctggcc ggaggatctc
     6301 aataaagatc tccaacaggt tgaaaagctg ataagcggtg aaataaacac ctattcaatg
     6361 gaaaaacgtt actacaaccg caatggcgat gttgtctggg cgttgcttgc cgtctcactg
     6421 gtgcgccaca cggatggcac gccgctctat tttatcgctc agattgaaga cattaacgag
     6481 ctaaaacgca ccgaacaggt caatcagcaa ctgatggagc gcatcacgct ggccaacgaa
     6541 gcgggcggga ttggcatctg ggagtgggag ctgaagccga atatttttag ctgggataag
     6601 cggatgttcg agctgtatga aattcctccg catatcaaac cgaactggca ggtgtggtac
     6661 gagtgcgtgc tgccggaaga tcgccagcac gccgaaaaag tgattcgtga ttcgttgcaa
     6721 tcacgctcgc cctttaaact ggaatttcgc attgccgtga aagatggcat ccgccatatc
     6781 cgcgccctcg ccaaccgggt actgaataaa gaaggcgaag ttgaacgcct gctcggcatt
     6841 aatatggata tgacggaggt taaacagctt aacgaggcat tgtttcagga aaaagagcgc
     6901 ctgcacatta cgctggactc catcggcgaa gccgtggtct gtattgatat ggcaatgaaa
     6961 attaccttta tgaatccagt ggcggagaag atgagcggct ggacgcagga agaagcgtta
     7021 ggtgttccgc tcctgacggt gttgcatatt acttttggcg acaacggacc attaatggag
     7081 aacatttaca gtgctgacac ctcacgttcc gcgattgaac aagatgtggt attgcactgc
     7141 cggagcggcg gcagttacga cgtgcattac agtattacgc cgttaagtac tctggacggc
     7201 agcaatatag gctcggtact ggtgattcag gacgttaccg aatcacgcaa aatgctgcgc
     7261 cagctgagct acagcgcctc ccatgatgca ctgacgcatc tcgccaatcg cgccagtttt
     7321 gagaagcaac tgcgtatcct gctgcaaacg gtaaacagta cgcatcagcg acatgccctg
     7381 gtgtttatcg atcttgatcg ctttaaagcg gtgaatgaca gcgccgggca tgcggcgggc
     7441 gacgctttac tgcgcgaact ggcgtcgttg atgctgagta tgctgcgctc cagcgacgtg
     7501 ctggcgcggc tcggtggtga tgaatttggt ctgctactgc cagactgcaa tgttgaaagc
     7561 gctcgtttta tcgctacacg cattatcagt gccgtgaatg actatcactt tatatgggaa
     7621 ggacgtgtgc atcgggtagg tgccagtgcc ggaattacct tgattgatgg caacaatcat
     7681 caggcagctg aagtgatgtc gcaggctgat atcgcctgtt atgcctccaa aaatggtggc
     7741 cggggccggg tgacgattta cgaaccgcag caagctgccg cacataacga acgggcagcg
     7801 atatcgcttg atgaacagtg gcggatgatt aaagagaatc agttgatgat gatcgcccac
     7861 ggcatcgctt cgccacggct cccggaagca agtaatttgt ggctgatttc acttaagctc
     7921 tggagttgcg aaagcgagat tattgatgaa caaacatttc gtcgtagctt cagcgatccg
     7981 gcacttagcc atgctcttga ccaacgggta tgccacgatt ttttccggca ggccgcaaaa
     8041 gcggttgcca gtaaaggctt aagcatcgcc ctcccccttt ccgttgctgg tttgagtagc
     8101 gccacgctgg tgaatgaact gcttgagcag ctggaaaata gccctctacc agcacggtta
     8161 ttacatctga ttattccggc agaagcgatt ttcgatcacg cagaaagcgt gcaaaaactg
     8221 cggctggcgg gatgtcggat cgtattcagt caggtgggcc gagatctgca aatcttcaac
     8281 tcgctgaaag caaatatggc agattacctg ctacttgatg gtgagttatg cgccaacgtg
     8341 cagggtaatt tgatggatga gatgctgatt acgattattc aggggcacgc tcagcgactc
     8401 gggatgaaaa ccatcgccgg gccagtcgtt ttacccttag tgatggatac gctttcgggc
     8461 atcggcgtcg atctgattta tggtgatgtg attgccaatg cccaaccgct ggatttgctg
     8521 gtgaatagaa gttatttcgc gattaactaa gggagcgttt gcccattgcc tgatgcgacg
     8581 ctaacgcgtc ttatcaggcc tacaaatcgc tcattcccca ggccggataa ggcgctcgca
     8641 ccgcatccgg cgaccaacgt catgcttcgt ctggttgcca gccttccgta taccagatat
     8701 gcaacagcgc ataagaacgc cagggcttcc agcgttcggc ataacggcgg atttgtgccg
     8761 gcgtcattcc cggaaatcgc tgtttaatca gataatcatc cggcagaaaa acatctttcg
     8821 cctgccagcc acgcaaagca aaataatttg cagtccagcg cccgataccc ggaaaggttt
     8881 gcagcgtttt catcgcttgt tctacgtcgc ccggtattgt cattggtaag gtgccctcca
     8941 gcgccgcatt tgccagatga atcagcgcct ctgcccgttt caacggcata cctaacgctt
     9001 ttaatgcctg caggtcggct actgccagcc gctgaggcgt ggggaagcag acataatccg
     9061 gaaaatcatc cagccgttcg ccataaagct gtgccactct ggaggtcaat tttgccgcca
     9121 tcgccacgct caccagttgg cctaaaatcg cccgcacgcc ctgctcaaaa gcgtcaacac
     9181 aaccaggtaa acgcaatcct ggccgcgccg cgcctaactt gcccaacgca ccgttaacaa
     9241 tctgcgggtt acattgcaga tcaaacaggc ggctcatttt cgccagacac tcagcggcaa
     9301 caggttctaa acctgcactt aaatttatgt gcagagtatg gcgggctata tccggaatag
     9361 cagtcaccac gccgcgatat tcgcctaccg ccagactacg ggcataataa ctgtccgcga
     9421 ccgtttccac accgctcacc gcacgggcgg cgagaaatcc caacatccac gaccagtcat
     9481 acggcggctg ccagttcagg gtatacatcg catctccttg ttaatccgct ttcagcataa
     9541 acgttattca gacgttacgc tttgctttca tattccggtt gtcgcgacgg caacatttcg
     9601 ctaaagtcac gccccttctt caccggcatg gggattattt cgtgtttatt ggttttgatt
     9661 acggtacagc aaactgttca gtggcggtca tgcgcgacgg taaaccgcat ttgctaaaaa
     9721 tggaaaacga cagcacgctg ctgccttcaa tgctttgcgc gccaacgcgt gaagctgtaa
     9781 gcgaatggct gtaccgccat catgatgttc cggcagacga cgatgaaacg caggcgctgt
     9841 tgcgtcgggc gattcgttat aaccgcgaag aagatatcga tgttacggcg aaaagcgtgc
     9901 agttcggtct ttcctcactg gcacagtaca ttgatgatcc agaagaagtg tggtttgtga
     9961 aatcaccaaa atcgttcctc ggtgccagcg gcttaaaacc gcagcaggta gcgctgtttg
    10021 aggatctggt ctgcgcaatg atgttgcaca ttcgccaaca ggcgcaggcg cagctgccgg
    10081 aggcaattac tcaggcggtg attggtcgcc cgatcaactt ccaggggctg ggcggtgatg
    10141 aagcaaacac ccaggcgcaa gggattctgg aacgcgcggc gaagcgtgcc gggtttaagg
    10201 atgtggtatt ccagtacgag ccggtcgcgg ctgggctgga ttacgaagcc accttgcagg
    10261 aagaaaaacg agtgctggta gtggatatcg gcggtggtac gactgactgt tcattgctgc
    10321 tgatggggcc gcaatggcgt tcgcgtctcg atcgtgaagc cagcctgctg ggtcatagtg
    10381 gttgccgtat tggtggtaac gatctggata tcgcactggc gtttaagaac ctgatgccgt
    10441 tactgggcat gggtggcgaa accgaaaaag gcatcgccct gccgattctg ccgtggtgga
    10501 atgcggttgc gatcaacgac gttcctgcgc aaagtgattt ctacagtagt gctaactgtc
    10561 gtctgcttaa cgatctggta cgcgatgccc gtgaaccgga aaaagtggcc ctgttacaga
    10621 aagtctggcg tcagcgttta agctatcgcc tggtgcgtag cgcagaagag agcaaaatcg
    10681 ccctttcaag tgtagcggaa acccgcgcct cactgccgtt tatcagcgat gaactggcta
    10741 cgctgattag ccagcaaggg ctggaaagcg ccctcagcca gccactggcg cggattctgg
    10801 aacaggtgca actggcgctg gataacgccc aggaaaaacc ggacgttatc tacctgaccg
    10861 gcggtagcgc ccgttcgccg ctgattaaaa aagcgctggc agaacagttg ccgggcattc
    10921 cgattgcagg cggcgatgac tttggctccg tcaccgccgg gctggcacgc tgggcggaag
    10981 tggtgtttcg ttaattgaaa aatggctcag caacggctta aatctgcctg cgcctgagcc
    11041 agtttttctg ccgcctgacg taacggttcc atcaacggca tggtccgtgc tggagcttga
    11101 aggcggaatc gctgcaactc tgtcgcaccg acagtcagcg ccgattccag ccgatgtcgt
    11161 ttggcggcca tttttgccat taccgcttgt ctgtctgcag gcgttaccgc ttcgttcggc
    11221 ctgaaaacaa aacggcgttc gcaactcgct ttccagtcga taaccgcctg ggtcagatga
    11281 tcaccaaaac ccctaacttg cttaacgctc cgtcgggtaa cgtccgctgc tgtttcaata
    11341 ccaaaggacc gtaacgccgc tttacgcgca gggccaacgc cgggaataga ggcaacatca
    11401 ataaaaaatc cctccagaaa cttctgcttc tgccgttccc ttgcggtgtc gtgaagtgct
    11461 gccagagcgc gtttctcttc ttccggtaac ccgagaattt cgtccttcat tttttcgagc
    11521 atcgcccgtt tggcgataaa accttccagc ccgcccaact gctggatctg gctgactaaa
    11581 tggtcatagt ccattttcgc acgattaaat gcctcacggc gttgctgtat ttctgctttg
    11641 tacgctttac ttgtcaggct gccaataatc cagatagccg ccagcacagg aatcaaaata
    11701 atataacgcg gttctgcctg aaggccgcaa agcaacagcg ataacgctga cagtgcgatc
    11761 tcaatcagaa tgatgtattc gcgccgtaac aggcctgaag gaagcagcct gccagtcggt
    11821 tggaaatgat cgggtaatgg cagttgcaat gctggcggtg ctactgatgc catcaccatc
    11881 gcccagactc tcgccagcac aaaatcaccg ccggtggtaa tgacctcttc gctgagatca
    11941 ataaaataga taacgccttg attatccagc gcacaccacg ggcagtcagc caaatgagcg
    12001 gggtaaacat gcattgccga aacggtacat ttctttaact gttggcgtag taaatcaagt
    12061 gccgctaccc acgcttttgc cgtcggacgc ccggttgcca cgccactttc cgtgaatgcc
    12121 tgctgaaaca tggcttcaac atcgcccggt aacatcgata gcggaatcga tcgtggcggc
    12181 ggttttaaac cacgtcgctg attatctgac gcgtaggcat aacggaaatg ggcaatatcc
    12241 atctccagcg cattacccgc atcagagata agcggtacac cggaataagg atgccgccca
    12301 ccaaacaaga tgtgaaatat cagcaacgca aggccaaaat tatcgtgatt ttctgtgcgt
    12361 tcaaagccga caaatgatgg catggtttgc agctctggcg gcgtaaaatg cgacacgccg
    12421 acttcgcata aatgcagtgt accattggca ttaatctgaa aggagtcgct atcaatcaac
    12481 accactttgc tgtcgcgacc taccataaag ctgttctggt ttacgtcacc cacgacgtgc
    12541 ccgtgctcat gaacagtagc aaaagatgaa gcaatattgc gcgcaacata gagtagaaaa
    12601 tcccacgcac aatgagggta actctggcga cgatgtgccg ggctatagat catatgaatc
    12661 ggttctttac cagaaacttt tggcatcata aaaccgataa cttttccgcc tcgtccaccg
    12721 tgaagcgttg cctgcggcca ggcgacataa ttcaacaact gcgcgtcagc tgtcgcagcc
    12781 ataaaggcaa gtttgtcctg ttttaaggcg ggtggcggcg tgtgataaat cttggcgacg
    12841 ctatcgacaa actcctcgat atcataaacc gcaccttcgc cgcctttgcc cagttcacgg
    12901 cctggcgtaa cgcactcacc agtagcagta tataaagtgg gtttcatggt aaccacatag
    12961 ccagtgccaa cgttttatca tcatccgtac gctcgtttac cgccgggctg gacaaaaaca
    13021 gctttaacaa ttcaggtaat aaatcgagtt gttcctgcgt tgctgacgcc agtccattga
    13081 aaaacggggt aaagaaaggc acatgcggag aattatccag catatttaac gccagtcgct
    13141 gaataccatc cgtaaatgct gcaactttat gcgcacgctc agtgctggtg aaagtttcca
    13201 gtctggaaac tgcatcttca tcggtaatga agtgcgtcat attggcatat tcacccacca
    13261 tcggcgtgag cggcagttgc aaaccgtgac ccaaatctac caccacgccg ccatcgccaa
    13321 tctgcataat caaggtgcca ttagctgatg aaatcaatcc caaaaacgtg caggcaaaat
    13381 cgcgaatcgc taattctttg gcttccgcct cggcaaaaag tcgctgacga atggtcagca
    13441 caatgtccgt tgcgaggaca tcattaagcc ccaattcccc gccctgcact ttttgcgaca
    13501 tataagccat cgcttcattg acggcgagca tcgcaccttc gccaccctgc gagacgctgc
    13561 ctgcgccatc agcgacaaat accgacaata atggctgctg gtcatttagc caggcaacct
    13621 gcatctggca ggcatcctgg caaggcaaat cagcgctaat atgcgacgtt ccaacggctg
    13681 atgcatagac cagtcgccag ctcacactga cgtccagcct tttggcgctt ccagcacaac
    13741 ttccgttccc ggcgtggagc gggaaaccga acgcagcgaa ctggataacc agctaaacag
    13801 ttcacggaat tgtaatccct gcagagacaa aggctgacgg acgctgattt gggccagcgt
    13861 cttcatatcc gccccctgaa caccgatggt aaagaaggca aacttcttat cttcttcccc
    13921 ctgaaacact ttgttggcgg cagcctgcca ctcatcggtt ggtgcgccat cggtgatcag
    13981 gaaaatccac ggacgataat aggaaatacc attggcacga tattcacgtt tccgctcctc
    14041 aaccatatcc agggctttgg taatggcagc gcccattggt gtatcgccct gggcaaacag
    14101 gatgggcggg aaaaaattag cggcgctggt aaagggttgt tccacatgca ccgggccgaa
    14161 cgtcacaatg ccgagttcca ctctttttaa ggccagcgaa tcggcaagca gctcatcgcg
    14221 aaaggtaacc aatccggtgt taagttcatt aatgggtctg ccgctcatcg agccagaaac
    14281 atccagcagc aaaatgcagg ggcaacgtgg ttccgggtta ctggcaaaat cgctggtggc
    14341 aaacgtgatt tgttcgctca taagaaatcc tttcttagag aattattgag gtccggtatc
    14401 ataccgattg caataatgaa ataagagcaa acgaagaaac tttcagaatt aggcccggcg
    14461 gcgcagaaaa ttttgctatt cgcctcgcaa gaacgtgggc ttacagattg acaacaaggg
    14521 ttgagtgaca taatttcagg agtgagggta gagcggggtt tcccccgccc tggtagtctt
    14581 agtatgctgg gtagctaatg actaagagca ccacgatgat gagtagcttc atcatgaccc
    14641 tttccttatt taaggcccct tcctcgggag gggctttccc gtttcagcgt cccgctgaaa
    14701 tcgtcggctt acctcctttc gccctgtatg cagtctatcg ctgacttgcg gataaaatag
    14761 tttcaagtgt gattattgcg taacgactcg caaagaatat ttgctggcag gatcgcagac
    14821 tgcaaagtct gcggattgac aattggaccg tgaaggcata ctttcaggaa tgagggttga
    14881 gcggggtttc ccccgcccta gtagtcttag taagccggga agcttatgac taagagcacc
    14941 acgatgatga tccacttcat catgaccctt tccttattta tggccccttc ctcgggaggg
    15001 gctttcccgt ttcagcgtcc cgctgaaatc gtcggcttat ctcctttcgc catgcaagca
    15061 gtctatcgct aacgcgtaga taaaatagtt tcatgtgtta ttactggatg cgtgctcgca
    15121 aaagtgcccg tcattcagac gattcccgac agtgtttcat aattcctcca tttttctccc
    15181 ttattggctg gctacactag tatcattccg cgaaacgttt caggaagaga aactcttaac
    15241 gatgaaaggc agttataaat cccgttgggt aatcgtaatc gtggtggtta tcgccgccat
    15301 cgccgcattc tggttctggc aaggccgcaa tgactcccag agtgcaaccc caggggcgac
    15361 gaaacaagcg cagcaatcgc cagcgggtgg tcgccgtggt atgcgtgccg gcccattagc
    15421 tccggttcag gcggcgaccg cagtagaaca ggctgttccg cgttacctca ccgggcttgg
    15481 caccattacc gccgctaata ctgttacggt gcgcagccgc gtggacggtc aattgatggc
    15541 gttacatttc caggaaggcc agcaggtcaa agcaggcgat ttactggcag aaattgaccc
    15601 aagccagttc aaagttgcat tagcacaagc ccagggccaa ctggcaaaag ataaagccac
    15661 gctcgccaac gcccgccgtg acctggcgcg ttatcaacaa ctggcaaaaa ccaatctcgt
    15721 atctcgtcag gaactggatg cccaacaagc gctggtcagt gaaaccgaag gtaccattaa
    15781 ggctgatgaa gcaagcgtcg ccagcgcgca attgcaactc gactggagcc gtatcaccgc
    15841 accagttgat ggtcgcgttg gtctcaagca ggttgatgtt ggtaaccaaa tctccagtgg
    15901 cgacaccacc gggattgtgg tgatcaccca aacacatcct atcgatctgg tctttactct
    15961 gccggaaagc gatatcgcca ccgttgtaca ggcacaaaaa gccggaaaac cgctggtggt
    16021 agaagcctgg gatcgcacca actcgaagaa gttaagtgaa ggcacgctgt taagccttga
    16081 taaccaaatc gatgccacta ccggtacgat taaagtgaaa gcacgcttta ataatcagga
    16141 tgatgcgctg tttcccaatc agtttgttaa cgcgcgcatg ttagtcgaca ccgaacaaaa
    16201 cgccgtggtg atccccaccg ccgctctgca aatgggcaac gaaggccatt ttgtctgggt
    16261 actgaatagc gaaaacaaag tcagcaaaca tctggtgaca ccgggcattc aggacagtca
    16321 gaaagtggtg atccgcgcag gtatttctgc gggcgaccgc gtagtgacgg acggaattga
    16381 tcgcctgacc gaaggggcga aagtggaagt ggtggaagcc cagagcgcca ctactccgga
    16441 agagaaagcc accagccgcg aatacgcgaa aaaaggagca cgctcctgat gcaggtgtta
    16501 cccccgagca gcacaggcgg cccgtcgcgc ctgtttatta tgcgtcctgt cgccaccacg
    16561 ctgctgatgg tggcgatctt actcgccggg attatcggtt atcgcgccct gcccgtttcg
    16621 gcgctgccgg aagtggacta tccaaccatt caggtggtca cgctttatcc aggtgcgagc
    16681 ccggatgtca tgacctctgc cgttaccgcg ccgctcgaac gccagttcgg gcagatgtct
    16741 ggcctgaaac agatgtcgtc gcaaagttcc ggcggtgcgt cagttatcac tttacagttc
    16801 cagctaacat tgccgctgga tgtcgccgag caggaagtgc aggccgcgat taacgctgcg
    16861 accaacctgc taccgagcga tctgcctaac ccgccggttt acagcaaagt gaacccggca
    16921 gatccaccga tcatgacgct tgctgtcacc tcaaccgcca tgccgatgac gcaggtggaa
    16981 gatatggtgg aaacccgcgt cgcgcagaaa atctcgcaga tttccggcgt agggctggtg
    17041 acactttccg gcgggcagcg tccggcggta cgcgtaaaac tcaacgctca ggcaattgcc
    17101 gctctcggcc tgaccagcga aagtgtgcgc accgctatta ctggcgcgaa cgttaactca
    17161 gcgaaaggga gccttgacgg cccttctcgc gcggtcacgc tttccgcgaa cgaccagatg
    17221 caatcggcag aagagtatcg tcagctaatc atcgcctacc agaacggcgc accaattcgt
    17281 cttggcgatg tcgctactgt agagcaagga gcagaaaaca gctggctcgg cgcgtgggcg
    17341 aacaaagaac aggccattgt gatgaatgtt cagcgccagc ccggtgctaa cattatctcc
    17401 accgccgaca gcatccggca gatgctacca cagctcaccg agagtctgcc gaaatcggtg
    17461 aaggtgacgg tactttctga ccgtaccacc aatatccgcg catcggtcga cgatacccag
    17521 tttgagctga tgatggctat cgcgctggta gtcatgatta tctacctgtt tttgcgcaat
    17581 attccggcga ccatcattcc cggcgtcgca gtgccgctgt cgttaatcgg caccttcgcg
    17641 gtcatggtgt ttctcgattt ttcaatcaat aacctgacgc tgatggcact aaccatcgcc
    17701 accggatttg tggtcgatga cgccatcgtg gtgatcgaga acatttcccg ctatatcgaa
    17761 aaaggcgaaa aaccgttggc ggcggcgctc aaaggcgcgg gtgaaatcgg ctttaccatt
    17821 atctcgctga ccttctcgct gattgcggtg ttgatccccc tgctgtttat gggcgatatc
    17881 gtcgggcgac tgttccgcga atttgctatt accctggcag tcgcgatttt gatctcggcg
    17941 gtggtgtcgc tgaccctgac accgatgatg tgcgcgcgga tgctcagcca ggagtcgctg
    18001 cgtaaacaga accgcttctc ccgtgcctcg gagaaaatgt tcgacaggat aatcgccgcc
    18061 tatggttgtg gactggcgaa agtgctgaat catccgtggc tgaccttaag cgtggcgctc
    18121 agcacgctgc tgcttagcgt gctgctgtgg gtattcattc cgaaaggttt cttcccggtg
    18181 caggacaacg gcattattca gggcactttg caggcaccgc aatccagctc ctttgcgaat
    18241 atggcccagc ggcaacgcca ggtcgcggac gtgattttgc aggatccggc agtgcaaagt
    18301 ttgacctcat ttgttggcgt tgatggaact aacccgtcgc tgaacagtgc gcgtttacag
    18361 atcaatctca aaccactgga tgaacgagat gaccgggtgc aaaaagtcat cgcccgtctg
    18421 caaacggcgg tggataaagt gccgggcgtc gatctcttcc tgcaaccaac gcaggacctg
    18481 actattgata ctcaggtcag ccgcacccag taccagttta ccttgcaggc cacgtcactg
    18541 gatgcgctca gtacctgggt gccacagttg atggaaaaac tccagcagct gccacagctt
    18601 tctgatgtct ccagcgactg gcaggacaaa gggctggtgg cgtatgtcaa tgtcgacagg
    18661 gacagcgcca gccgcctggg gatcagcatg gcggatgtcg ataacgccct gtacaacgcg
    18721 tttggtcagc gactgatttc cactatttat actcaggcta accagtatcg cgtggtactg
    18781 gaacacaaca ccgaaaatac cccaggcctc gcggcgctgg ataccattcg cctgaccagc
    18841 agcgacggtg gcgtggtgcc gttaagctca attgccaaaa ttgagcagcg ttttgcgccg
    18901 ctttccatca accatctgga tcagttcccg gtaacgacta tctcctttaa cgtgccggat
    18961 aactattcgc tgggcgatgc ggtgcaggcg attatggaca cagagaagac gctgaatctg
    19021 ccggtggata tcaccacgca gttccagggc agcaccctcg ccttccagtc ggcgctgggc
    19081 agcacggtct ggctgattgt cgcggcggtg gtggcgatgt atatcgtgct cggcattctg
    19141 tacgagagct ttattcaccc gatcaccatt ctctcgacgc tacccaccgc aggggtcggc
    19201 gcgctgctgg cgttaatgat tgccggtagc gaactggatg tgattgccat tatcggcatt
    19261 attttgctga tcggtatcgt gaagaagaac gctatcatga tgatcgactt cgcgctggct
    19321 gctgagcgcg agcaaggcat gtcgccgcgc gaagctattt accaggcttg tctgttgcgt
    19381 tttcgtccga tcctgatgac cactctggcg gctctgcttg gtgcgctgcc gctgatgttg
    19441 agtaccgggg tcggcgcgga actgcgtcgt ccgttaggta tcggcatggt cggcggtctg
    19501 attgtcagcc aggtgctgac gctgtttacc acgccggtga tttatctgct gttcgaccgt
    19561 ctggcattgt ggaccaaaag ccgctttgcc cgtcatgaag aggaggcgta agtgaagttt
    19621 tttgccctct tcatttaccg cccggtggcg acgattttac tgtcggtcgc cattaccctg
    19681 tgcggcatac tgggtttccg tatgctgcca gtcgccccgc tgccgcaggt cgattttccg
    19741 gtgatcatgg tcagcgcctc gctgcccggt gcgtcaccag aaacaatggc gtcttccgtt
    19801 gccacgccgc tggagcgctc acttgggcgc attgccggag tcagcgaaat gacctccagc
    19861 agttcgctcg gcagcacgcg cattattttg cagtttgatt ttgaccggga tatcaacggc
    19921 gcagcgcgcg atgtgcaggc ggcgatcaac gccgcgcaaa gtctgctacc cagcgggatg
    19981 cccagccgtc cgacctatcg caaagcgaac ccgtcggatg cgccaattat gatcctcacg
    20041 ctgacatccg atacttattc gcagggtgaa ctgtacgatt tcgcctcgac acagctggct
    20101 ccgacgattt cgcaaatcga cggtgtcggt gatgtcgatg ttggcggcag ctcgctgccc
    20161 gccgtacgcg tcgggctgaa cccgcaagcg ctgtttaatc agggcgtgtc gctggacgat
    20221 gtgcgcaccg ccatcagcaa tgccaacgtg cgtaaaccgc agggggcgct ggaagatgat
    20281 actcaccgct ggcagatcca gactaacgat gaactgaaaa ccgccgctga atatcagccg
    20341 ctgattattc attacaacaa cggcggcgcg gttcgtctgg gcgatgtggc gacggtgacc
    20401 gattcggtgc aggatgtgcg caacgccggg atgaccaacg ccaaaccggc tattctgctg
    20461 atgatccgca aactgccgga agccaatatt atccagacgg tagacagcat ccgcgcccgt
    20521 ctgccggaat tacaatccac catcccggca gcaattgatc tgcaaattgc ccaggatcgc
    20581 tcccctacta tccgcgcgtc gctggaagag gttgagcaga cgctgattat ctcggtggcg
    20641 ctggtgattc tggtggtgtt tttgttcctg cgttcgggtc gcgccaccat tattcccgcc
    20701 gttgcggtgc cggtttcgct gattggtacg tttgcggcga tgtacctgtg tggtttcagt
    20761 ctcaataatc tttcgttaat ggcgctcacc atcgccactg gctttgtggt ggatgacgcc
    20821 atcgtggtgc tggaaaacat tgcccgtcat ctggaagcgg ggatgaaacc gctacaggcc
    20881 gcgctgcaag gtactcgcga agtcggcttt acggtgctgt cgatgagtct gtcactggtg
    20941 gcggtgttcc tgccgctgct gttgatgggc ggattgccgg gtcgcctgtt acgcgagttt
    21001 gccgtgacgc tttctgtcgc cattggtatt tcgctgctgg tttccctgac gttaacgccg
    21061 atgatgtgcg gctggatgct aaaagccagc aagccgcgcg agcaaaagcg actgcgtggc
    21121 tttggtcgca tgttggttgc tctgcaacaa ggctacggca agtcgctaaa atgggtgctc
    21181 aatcataccc gtctggtggg cgcggtgctg cttggcacca ttgcgctgaa tatctggctg
    21241 tatatctcga tcccgaaaac cttcttcccg gagcaggaca ccggcgtact gatgggcggg
    21301 atacaggctg accagagtat ttcgtttcag gcgatgcgcg gtaagttgca ggacttcatg
    21361 aaaattatcc gtgacgatcc ggcggtggat aatgtcaccg gctttaccgg cggttcacgg
    21421 gtgaacagtg gaatgatgtt tatcaccctt aaaccacgcg gcgagcggag cgaaacggcc
    21481 caacagatta tcgatcgctt gcgtaaaaaa ctggcgaaag aaccgggagc gaatctgttc
    21541 ctgatggcgg tacaggatat tcgcgttggc ggacgtcagg ctaacgccag ctaccagtac
    21601 acattattgt ccgacgatct ggcggcactg cgcgaatggg agccaaaaat ccgcaaaaaa
    21661 ctggcgacat tgccagagct ggctgatgtt aactccgatc aggaagataa tggcgcggag
    21721 atgaacctta tttacgatcg cgacactatg gcgcgattgg gaattgacgt gcaagccgcc
    21781 aacagcctgt taaataacgc cttcggtcag cggcaaatct cgaccattta ccagccgatg
    21841 aaccagtaca aagtggtgat ggaagtggat ccgcgctata cccaggacat cagtgcactg
    21901 gaaaaaatgt tcgttatcaa taacgaagga aaagcgatcc cgctgtcgta tttcgctaaa
    21961 tggcaaccgg cgaacgctcc actgtcggtg aatcatcagg gattatcggc ggcctcgacc
    22021 atttcgttca acctgccgac cgggaaatcg ctctcggacg ccagtgcggc gatcgatcgc
    22081 gcaatgaccc agcttggtgt gccttcgacg gtgcgcggca gttttgccgg aacagcgcaa
    22141 gtgttccagg agacgatgaa ctcacaggtg atcctgatta tcgccgccat cgccacggta
    22201 tatatcgtac tgggtatcct ttacgagagt tatgtgcatc cactgacgat tctctccacc
    22261 ctaccctcgg cgggcgtcgg agcgctgttg gcgctggagc tgttcaatgc cccgttcagc
    22321 ctaattgcgc tgatagggat catgttatta atcggcatcg tgaagaaaaa cgccattatg
    22381 atggtcgatt ttgcgcttga agcccaacgg cacggtaacc tgacgccgca ggaagctatt
    22441 ttccaggcct gtctgctgcg ttttcgcccg attatgatga ctaccctggc ggcgctgttt
    22501 ggtgcgctac cgctggtatt gtcgggcggt gacggctcgg agttgcgcca accattgggg
    22561 atcaccattg tcggcggtct ggtcatgagc cagcttctga cgctgtatac cacgccggtg
    22621 gtgtatctct ttttcgaccg tctgcgactg cgtttttcgc gtaaacctaa acaagcggta
    22681 accgagtaaa tgacagatct tcccgacagc acccgctggc aattgtggat tgtggctttc
    22741 ggcttcttta tgcagtcgct ggacaccacc atcgttaaca ccgcgcttcc ctcaatggcg
    22801 caaagcctcg gggaaagtcc gttgcatatg cacatggtca ttgtctctta tgtactgacc
    22861 gtggcggtga tgctgcccgc cagcggctgg ctggcggaca aagtcggcgt gcgcaatatt
    22921 ttctttaccg ccatcgtgct gtttactctc ggttcactgt tttgcgcgct ttccggcacg
    22981 ctgaacgaac tgttgctggc acgcgcgtta cagggcgttg gcggcgcgat gatggtgccg
    23041 gtcggcaggt tgacggtgat gaaaatcgta ccgcgcgagc aatatatggc ggcgatgacc
    23101 tttgtcacgc tgcccggtca gatcgggccg ctgcttggcc cggcgctcgg cggtctgctg
    23161 gtggagtacg catcgtggca ctggatcttt ttgatcaaca ttccggtggg gattattggc
    23221 gcgatcgcca cactgatgtt aatgccgaac tacaccatgc agacgcggcg ctttgatctc
    23281 tccggctttc tgttgctggc ggttggcatg gcggtcttaa ccctggcgct ggacggcagt
    23341 aaaggtacag gtttctcgcc gctggcgatt gctggcctgg tcgcagttgg cgtggtagca
    23401 ctggtgcttt atctgctgca cgcccaaaat aacaaccgcg ccctgttcag tctgaaactg
    23461 ttccgcactc gtaccttttc gctgggcctg gcggggagct ttgccggacg tattggcagt
    23521 ggcatgttgc cctttatgac accggttttc ctgcaaattg gtctcggttt ctcgccgttt
    23581 catgccggac tgatgatgat cccgatggta ctcggcagca tgggaatgaa gcgaattgtg
    23641 gtacaggtag tgaatcgctt tggttatcgt tgggtactgg tggcgaccac gctgggcctg
    23701 tcgctggtca ccctgttgtt tatgactacc gctctgctgg gctggtacta cgttttgccg
    23761 ttcgtcctgt ttttacaagg aatggtcaac tcgacgcgtt tctcctccat gaacaccctg
    23821 acgctgaaag atctcccgga caacctggcg agcagcggca acagtttgct gtcgatgatt
    23881 atgcaattgt cgatgagtat tggcgtcacc atcgccgggc tgttgctggg gctttttggt
    23941 tcgcagcatg tcagcgtcga cagcggcacc acacaaaccg tctttatgta cacctggctt
    24001 agcatggcgt ttatcatcgc ccttccggcg tttgtctttg ccagagtgcc gagcgatacg
    24061 catcaaaatg tggctatttc acggcgaaaa aggagcgcgc aatgaagttc tggcgacccg
    24121 gtattaccgg caaactgttt ctggcgattt tcgccacctg cattgtctta ttgatcacga
    24181 tgcactgggc ggtacgtatc agttttgagc gcggctttat cgattacatc aagcatggta
    24241 atgaacaacg gctgcaaatg ctcggcgatg cgcttggtga gcagtacgcc cagcacggga
    24301 actggcgctt cctgcgtaat aacgatcgct ttgtatttca gatcctacgt tcactggagc
    24361 atgataacaa cgaagataag cccggccccg gtatgccacc acacggctgg cgcacgcaat
    24421 tttgggtggt tgatcaaaat aataaagtgc tggttggccc gcgagcaccg gttccacccg
    24481 acggcacacg gcggcccatt atggtcaatg gtgcggaagt tggcgcggtg atcgcctccc
    24541 ctgttgaacg actgacccgt aatactgata tcaattttga cagacaacag cggcaaacca
    24601 gttggctgat tgtggcttta tctaccttat tagcggcgct ggcgacattc ccactggcgc
    24661 gcggtttgct ggctccggta aaacgactgg tggatggcac gcacaaactg gcagcgggtg
    24721 atttcactac tcgcgtagcc cccaccagcg aagatgagtt gggcagactg gcagaggatt
    24781 tcaatcaact cgccagcaca ctggagaaaa accagcagat gcgccgcgat tttatggccg
    24841 atatttctca tgaactgcgc acgcctttag cagtactgcg cggcgaactg gaagccattc
    24901 aggatggtgt gcgtaaattc acgccggaga cgctggcttc tttacaggca gaagtcggaa
    24961 cactgacaaa actggttgac gatctccatc aattgtctat gtctgatgaa ggcgctctcg
    25021 cctatcaaaa agcaccggta gatttgatcc cactgctgga agtggcggga ggcgcatttc
    25081 gcgaacgatt cgccagccgt ggcctgaaat tgcaattttc cctgccagac agtattaccg
    25141 tatttggcga tcgcgatcgt ttaatgcagt tgttcaataa cttactggaa aacagcctgc
    25201 gctacactga cagcggcggc agcctgaaaa tctctgccga acagcacgac aaaacggtgc
    25261 gcctgacctt tgccgacagt gcgccgggtg tcagtgacga tcagctacaa aaattgtttg
    25321 aacgttttta tcgcaccgaa ggttcccgca accgcgccag cggtggttcc gggctggggc
    25381 tggcgatttg cctgaacatt gttgaagcac ataacggccg cattattgcc gcccattcgc
    25441 cttttggcgg gcttagcatt acagtagagt taccgctgga acgggattta cagagagaag
    25501 tatgaccgag ttaccaatcg acgaaaacac accgcgtatt ttgatcgtgg aagatgaacc
    25561 gaagctgggg cagttgctca ttgattatct gcgtgctgcg agctatgcgc caacgttgat
    25621 tagccacggc gatcaggtac tgccatatgt gcgccagaca ccaccggatc tgatcctgtt
    25681 ggatctgatg ctccctggca ccgatggcct gacgctgtgc cgggaaattc gtcgtttttc
    25741 tgacattccg atcgtgatgg tgacggcaaa aatcgaagag atcgaccgcc tgctggggtt
    25801 ggagattggc gcagatgact atatctgcaa gccgtacagc ccacgggaag tggtagcgcg
    25861 cgtcaaaacc attttgcgcc gttgcaaacc gcagcgtgag ttgcagcaac aggatgctga
    25921 aagcccgttg attatcgacg aaggtcgttt tcaggcttcc tggcgcggta aaatgcttga
    25981 cctgacgcct gcagaatttc gtctgctgaa aacgctctct catgaaccag gaaaagtgtt
    26041 ctcccgcgag cagttgctga accatcttta tgacgactac cgcgtggtaa ccgaccgtac
    26101 catcgacagc cacattaaaa acctgcgccg caagctggaa tctctcgacg ccgaacagtc
    26161 atttatccgc gccgtttacg gcgtcggtta ccgctgggaa gccgacgcct gccgcatcgt
    26221 ttaacatttt tacctgccgg agatggcctt cagaccagat ccggcaacat tatcccacgc
    26281 atggtcagca aactgacatt atctctccgc cctggcaggc accagaaaat cgctcatact
    26341 ttaattggca aacagtacct ttagatgctg ttttcgatac gcaatttcaa tcaaggagtc
    26401 attatggctg gttggtttga actcagtaag agcagtgata atcagttccg gtttgtgcta
    26461 aaagcgggca atggtgagac tatcctcacc agcgagctgt atacctcaaa agcctctgcg
    26521 gaaaagggca tcgcgtcggt gcgtagcaac agcccgcaag aagaacgcta tgagaaaaaa
    26581 acggcaagca atggcaaatt ctatttcaat ctgaaagccg ccaatcatca aattatcggc
    26641 tccagccaga tgtatgccac cgcgcaatct cgtgaaaccg gaatagcctc cgttaaagca
    26701 aatggcacaa gccagacggt gaaagacaat acgtaattac gatgccgggc gtaggttgtt
    26761 ggcgtccggc gctttaccag ttacccccac agcgctacaa tgcccgccct taaagtgggg
    26821 gcactcccct aaccgcttca tcaggtgaag cggctctgac ctgtcatcag aacgagagaa
    26881 ttatgtttaa accggaactc ctttccccgg cgggaacgct gaaaaatatg cgttacgctt
    26941 tcgcttatgg cgcagatgct gtttatgcgg gccagccgcg ttactccctg cgtgtacgca
    27001 acaacgaatt caaccacgaa aatcttcagc tcggcatcaa tgaagcccac gcgctgggga
    27061 aaaagtttta tgtcgtggtc aacattgcac cgcacaacgc caagctgaaa acctttatcc
    27121 gtgacctgaa accggtggtg gaaatggggc cggatgcgct gattatgtcc gatccagggc
    27181 tgattatgct ggtgcgtgag cacttcccgg aaatgccaat ccacctctcg gtacaggcta
    27241 acgccgtaaa ctgggcgacg gtgaaattct ggcagcaaat gggtctgacc cgcgtgatcc
    27301 tctctcgcga actgtcactg gaagagattg aagagatccg caatcaggtg ccggatatgg
    27361 agatcgaaat cttcgttcac ggcgcgctgt gcatggccta ctccggtcgc tgcctgctct
    27421 ctggctatat caacaagcgc gacccgaacc agggcacctg caccaacgcc tgccgctggg
    27481 agtataacgt ccaggaaggg aaagaagatg acgtcggcaa catcgtacac aagaacgagc
    27541 cgattccggt gcaaaatgtt gagccgacgc tgggtatcgg cgcgccaacc gacaaagtgt
    27601 ttatgatcga agaagcccag cgtccgggcg agtatatgac cgcgtttgaa gatgagcacg
    27661 gcacttacat catgaactcg aaagatctgc gcgccatcgc ccatgttgaa cgcctgacca
    27721 aaatgggcgt gcattcgctg aaaatcgaag gccgtaccaa atctttctac tattgcgcac
    27781 gcaccgcgca ggtttaccgt aaagctatcg atgacgccgc tgcgggcaaa ccgttcgata
    27841 ccagcctgct ggaaactctg gaaggtctgg cgcatcgtgg ctataccgaa ggtttcctgc
    27901 gtcgtcatac ccacgacgat tatcagaact acgaatacgg ttattcggtt tctgaccgtc
    27961 agcagtttgt tggtgagttt accggtgagc gcaaggggga gctcgcggcg gtagcggtga
    28021 aaaataaatt ctccgttggc gacagccttg agctgatgac gccgcaaggc aacattaact
    28081 ttacccttga gcacatggaa aacgccaaag gtgaagcaat gccggtcgca ccaggcgatg
    28141 gttatactgt gtggctcccg gtgccgcagg atcttgagct aaattacgcg ctgctgatgc
    28201 gtaatttctc cggggaaacc acgcgtaacc cccacggtaa gtgattaatt tcgattattt
    28261 ttcccggatg gaaaattctt agaaaccgat cacatacagc tgcatttatt aaggttatca
    28321 tccgtttcgc tgaaaaacat aacccataaa atgctagctg taccaggaac cacctcctta
    28381 gcctgtgtaa tctcccttac acgggcttat tttttatgca taagccctat ccctggtcac
    28441 cgtcttccat tgaccacatc gatagaatcc tccttcatag cacgatgcct ttcacttatc
    28501 gacatcgtgc tcgcacaggt tccggttacg cacagccaga acgcgcatgt ttgacgctta
    28561 ccaaaaaatg aacctaaagc attggaatat ttttgacatc attttctgat ggctgcataa
    28621 aataaaaatt ctgctttagt ttcatctatc tgtttgtcat taatactcac attcaataat
    28681 agtgttgaag aatatcccat cacagataaa ataaatatgt gcatggtagt cttgaatcta
    28741 ttctcactct ccacatttga atgtcagacg agcgacgcca tgtaatcctg caccttctgt
    28801 cttcaggtca actatctgca tttttttgcc ctgagtaaca cagaaatgag ttgcatcatt
    28861 ttttactata ttttctgcac cagatattct acccctggct aaagaagctt cggcttcggt
    28921 gtagtattgg ttatcgagtt tacgctgaat attactttta tatgcaaggc caaatttacc
    28981 gatacttgtc tcatcattat gcacagcaca accagacata agaaaaacac taattaatga
    29041 tatagcagct atctttttca ccttaccttc ccccattaat taccaacgac attctctagt
    29101 gtttaaatat aataatggca tgattattat aattgaatag gattataata aatgttctgt
    29161 acagcatttc ctacataagt aggaattaca gacatggagg cccttccggg caaccccatg
    29221 ggggacttta atatattttg aatagtatat tgaagatgcc actgtttagt tgaatattag
    29281 gtatattctc ttttttaaaa tttatctgtg gctaccgtta atattcgctg tccccattgt
    29341 aagctcctgg tggtaagcac tgaatcccca tacctgaact gactttttat cctccgactt
    29401 tcatcctgtt ctgactccac cttttctttt ctgctctaca ctatctacag accaatcata
    29461 aaggcacata cgatcatggc agaatttccc gccagcttac tgattcttaa tggcaaaagt
    29521 actgacaatc tacccttgcg cgaagcaatt atgctgttgc gtgaggaagg aatgacgatc
    29581 catgtgcggg ttacctggga gaaaggtgat gccgtacgat ttgtagagga ggcccggaag
    29641 ttaggtgtcg caacagtgat tgccggtggt ggcgatggca ccattaatga agtttctacg
    29701 gcattgattc aatgtgaggg ggatgaaata cccgcgctgg gaattttacc attaggaact
    29761 gccaatgatt ttgccactag tgtagggatt cctgaggcac tggataaggc gctgaaactg
    29821 gcaattgccg gtaacgccat tgcgatagat atggcgcaga tcaacaaaca aacctgtttt
    29881 attaatatgg cgacaggcgg gtttgggacg cgtattacca cagaaacgcc ggaaaaatta
    29941 aaagccgcgc tgggtggcgt ctcttacatc attcatggct taatgcgcat ggacactctg
    30001 caaccggacc gttgtgaaat ccgcggtgaa aactttcact ggcaaggtga cgccctggtc
    30061 attggtattg gtaacgggcg tcaggccggt ggcggtcaac aattgtgccc gaacgcatta
    30121 attaacgatg gcttgctgca actgcgcatt tttaccggcg atgaaattat tccgactctc
    30181 gtatcaacct taaaatctga cgaagataac ccgaatatta tcgaaggagc ttcgtcgtgg
    30241 tttgatatac aagccccaca cgaaatcact tttaatcttg atggcgaacc gttgagtggg
    30301 caaaacttcc atattgaaat acttccggcg gcgttgcgtt gtcgattacc accagattgt
    30361 ccattgttgc gttaatcaaa ctattctctg ttgaaccaga gagaatccgt aatgccggat
    30421 acggtgtaaa caccttatcc ggcatacaga acaatactac accgtaatta attttacccg
    30481 tgtcttctgc aatgccagtt tatccacccc agacacagca tcatcggtaa taataatgtc
    30541 aattttttcg attggcagta cctgattaaa accgcgacga ttaaatttcg atgaatcaag
    30601 aacggcaatc actttattcg ccgctgtcac catcgcaccg ctaacggaat aaccttcatt
    30661 aaaggtcgta ataccattaa ccgcatcaat accatcagca ccgacaaaca ttaaatcggc
    30721 attaatatcc tgcagggaac gttcagcaat agatccatgc atagatcgcg ttttatggcg
    30781 gaccgtgcca ccgcagacga ccagagtaat gtctttattt tcggaaaggg caaacgccgc
    30841 cgggagactg ttggtaatca ccgtgatatt tttggcggtc attaatcctt cggcgataag
    30901 cattgtggta cttccgctgt cgagaataac ggtcatccct tcgtggatca ttttgactgc
    30961 cgcctgcgct attctgtttt tcggcgcgct ggcgagctga aatcgctctt taaacccgac
    31021 ttcctgggtc tcggtttcgc tattaccgga cattattttc gccgcaccgc catgaaagcg
    31081 cgtaaccacg cctttttgtt cgagaaagcg caaatcggca cggattgtcg cttccgaggc
    31141 agcaaatact cccgccagat cctgaacaag cacggttccc tgttcattca ctaattggat
    31201 gatcttattc cttcgctcga atgaattcat cgtcatttac ccatattcaa ttgtggctag
    31261 tgtaaacgaa gggggaccat tggtgaatga tctgttttgc acaataccgg gcctgcatca
    31321 cgctggcccg cggtttcagg gaatgagcaa cactttgccc ggcatggcat tacgagcgat
    31381 gtcacgcacc gcctgggcga agctttcaaa gcttccacgg tgagcgatta atggctccag
    31441 gcttaactta cgttctgtca gcaaccggct cgccgtttcc cactcctgcc ccggccaagg
    31501 gctggagtag ttcatccaac tgccgataac cgtcagctct ttacgcaata ttttgccaaa
    31561 cgttgcagat gttaaatgca gatcctgatg caacgtgcct accagcgcca gttgggcatg
    31621 aggaccggca atctctaccg ccagttcgac ggtttgcggg acgccagccg tctcgaggat
    31681 aagctgatta aagcgcagat cgcgtaaaac gccctgtatt tgcggcgcgc tcatttcacg
    31741 gctgttaaat gtttgcatcg caccgaaaga ttttgccagt gccagttttt ctgagctaat
    31801 gtcgatagcc gtcacactct ttgctcccaa cgcgacagcg cactgaattg ccagcaggcc
    31861 aatggttccg gcaccaataa taataacgtt tttattctca catccttgcg ctaaatggaa
    31921 cgcatgcagg ccaacggtaa tcggttcaat aaaagcgcca tcctcaatag gcatatccgc
    31981 aggtagtgca aagacattgt ttcgcttaac gacaatatat tcggcaaatc caccatcacg
    32041 ccgtgagcca ataaaatcat attttgcgca ctgggaataa aaccctttca gacactctgg
    32101 acaagtaaaa caggggaata acggcacaca ggcaaccgca tcgccggggt gtaaatcatc
    32161 aacaccggat cccaccgcat caatatagcc actaaattca tggcctaacg ttattggata
    32221 ataatgtgca ccatttttaa atatcctggg taaatcggaa ccacataagc ccgagctggc
    32281 aatttttacc cgcacctcat cctgatgttt aatttcagga atgacgcttt ctgctacgcg
    32341 cacgatacca tcagtatcat tcaccactga tttcataaaa actcctgatt gttaaggggg
    32401 ataacctccc cctcaggtaa aaattattct gcgagaacgg ctttctcttg tttaataaaa
    32461 ccacgcgctc tacgccaggt catgaaaata ccggtaagat aaattgcgcc gataacaatg
    32521 aaaccgggaa tattttgcgg ggagaaaacc tgaatcagta accaggtaat gggagaaccg
    32581 ccctgatcca ttgaagccac catacccccg gcttttaacg ccccagcatt agccgccagt
    32641 tgggtgtgca aaccaatagt ttgcgtcgcg atccacaggg tgatgctcat aatgatgaca
    32701 cccgagatta aggtgcggaa cagatttcca cgatgcaccg cgaccgccat cgctacaaag
    32761 aaaccgatgg tggcaaggtc gccaaacggc agtacctgat tacccggaac acaaacagca
    32821 attaaaatgg tgagcgggat aaaaatcagg cttgccgata ccaccgccgt atgccccagc
    32881 agcaacgccg gatcaaggcc aatcaggaac tcctgaccgc cgaacttcgc ctgtaaacga
    32941 ctacgggcct gcttagcgat gggcgttaaa ccatccatga tgggtttaat cacccgtggc
    33001 atcagcagca taactgccgc cgtttttacc gccagctgca atacaccttt gacatcgtaa
    33061 cccgcgagga tgccgataat cagccccatc acaaagccca cggtgacagg ttcgccaaat
    33121 gggccaaatt tgcgctgaat atcgtcggcg ctaaatttaa tacggttaac gcctgggatt
    33181 ttctcgatga tagcatccac cagcaccgca attggcccca gatacgccga cgtaccgtgc
    33241 ggaatagcaa tgccttccag ctcaaagaaa tttcgggtat cgcgggcaaa ccagtcgccg
    33301 agcttataaa caaacgccgc gtgaattacc acacccgcca tccctatcat ccatgaaccg
    33361 gttgccagat gcagcaatgc gccggtgaag gtcatatgcc agatattcca gatatcaaca
    33421 tttaccaccc gcgtcatacg ggtcagtagc atcgccacgt taaccagaat cgcaatcgga
    33481 atcgctacca gcgcaatttg cgacgcccag gtcattggtg aagagcccgg ccaaccgaca
    33541 tcgaccacat gcagattcag gtcaaaattt tccgccatcg ctttcgccgc cgggccaatg
    33601 gaatccagca ttaagtcaat cacaaggcca ataccaacaa agccaatccc gatatgcaga
    33661 cctgctttaa agcaatcgcc tgccttcatg cctaatattt tagaaaaaat aatgatgaca
    33721 atcggcagca tcaccgtagg gccgaggtcg agaatataac gcatgacttc tgaaaacata
    33781 ggtcacccct gtaagatagt cagaatttta ttttgtaatg cttcgatacc gacaccagaa
    33841 acaaaaggca tgccgtgaac taacggaata tcgccaaaac ttcgatccac cctggcagtg
    33901 gtgcatatca aatgcacgcc atccatatag gtttctattt cattaactcg acactggatt
    33961 aattcaacag gaatattatg actctgacac aactctttaa tttcttccgc cgccatcgta
    34021 gaggtcgcaa ccgcgcctcc gcaagcgaca ataatcttgc gtttcataaa tagtcctttt
    34081 tattgttaca gagggattta ttcgtctgaa tctaaaacat actttgtgaa gtattccttt
    34141 aactgggttt caggaagagt gattagtgtc tcgacgatct cgggctgttg taacttacca
    34201 aataaacagc gtaaaagttt caattgctgc tgcggatttt ccacaattaa cgcaataacc
    34261 aacgataccg ccacgtcgtt atcatcatcc gcttgctgaa aatgaacttt atttgttggc
    34321 cttaacagat aaatggctga cgacttagca tgaatcgcct cacaatgcgg tattgcaatg
    34381 gcgtgctgct caagcattat cccggtaggg aattctgctt ctctggcaat taatgcctgt
    34441 ggccaggtat catgaaccac acctttcgcg agcatctcat taccgatatg ggttaaaact
    34501 tcgctacgat cgacaaaaga aattccgcta cgaacaaaca ggttagtcat ataccgtcct
    34561 tattccgcac tgccgtagcg ataggcgcgc aaaacatcat aaattttatc cataatcaac
    34621 tgatgcggta ttgctgataa ttccccggac tgaatgcgtt caaattgttt aggaagatac
    34681 tgactaatca tgcccagagg gatttccatg ccttgcagat tcaccatcat cgtttcgacg
    34741 ctatttttaa tccgactatg cggccagtaa taacgaatac gatccgacag gctgtaacga
    34801 atatccagta atgaatcgtt aaaacccgta cgataatatt ttttccagta ttgcggttcg
    34861 tcaaacatca cttcttcaat taccgccaag caaccgctgc gattttctgg agcgatgagt
    34921 tcctgctcaa tttgtgccag cgcaaatatc gcctcacgta aagcaaaggt taatgccgga
    34981 ccgactttca aaattgcaaa atgatcgcga actaattccc gataggccgt ctgagtctgg
    35041 taatcggtgg aatgcgcttc ataaaccatt ttggtttttt ctatccattg cgccagcgcc
    35101 tgagcttctt gcgcctgata atgaataata ttgctatggt caaattccac gcccggctgc
    35161 accacgatgg caatcacgcg tgttaacgcc tctgccagcc cacgggcaat aaaggccttt
    35221 tgatgcgtac gtaaagtatt ggcggcatct tcaacctggg tgatgtgtac tgactgaatg
    35281 gcgctggcct caccgcccgg aaccggaact tcggtgccaa tgacatagct cagttgctca
    35341 cgctggcaat ctgtcgccac actttccgca gcaaagcaaa gcacagcagc tcgtcccgca
    35401 accgtttctg gggctaacgg tataggatcc cccgcgcaag acattgacgc atcaagatga
    35461 attttactga accctgcacg cacgtatgcc tttaccagtt cgacggattt ttccatcgcc
    35521 gcatccgcat tttcttgctg ccagcagttt ggtcccagat ggtcgccacc gagaataata
    35581 cgctcacgag caaatccgac tttatcggca atcgcaaaaa caaattcgcg aaagtctgcc
    35641 ggtgtcattc cggtataacc gccaaattga ttgacctggt ttgacgttgc ttcaatcagc
    35701 actttgcgcg tgctgttgcg atcaaatgcc agcgccgctt cgataaccaa cggatgggca
    35761 gaacagactg aacatatgcc gatatgttca ccaactttat gccgggcaat taacgttttc
    35821 atattttttc cttgttaaat ggcaggtgcg ttatgccctg ccctcgcagc cacaatcggc
    35881 aatcactttg ctcaccacat cgcgcattgc agatttagcc gactgcaaat aatcacgggg
    35941 atcggtcgct tcggggtgct cggtcaggta attttttaac gcctgcgaga aggcattttt
    36001 tagctctgtt gcaacgttga ttttgcatat ccccagtttg atggtttgct gaatatcctt
    36061 agtcgataat cctgacgcgc catgcagcac cagcggtaaa ttcacccact ggcgaatgtt
    36121 ctccagtcga gaaaaatcaa gcgccggtgc tctggcatac ataccgtgag ccgtgccgat
    36181 ggcgaccgcc agggaatcaa tcccggttgc ctcggcaaat tcacgcgcct gaacggggtt
    36241 ggtataaaaa gcatcggctt cattgacttg cacatcatct tcctggccgc caagttgccc
    36301 cagctccgcc tcgacgctga catcaaagcg atggcaaaaa tccaccacct ctttgacccg
    36361 tgaaatattt tgcgcaaaag gcaaatgcga ggcgtcaatc atgactgagc gcacgccaga
    36421 acgaaccttc tgagcgatat cgtcaaattt cgtgtgatgg tcgagatgaa ttgtcagtgg
    36481 atggtgatat tgcttcgcca tcgcgctgac cagcgccaac agattttctg taccagcatg
    36541 agtaaatgtg ccaggcgttc cggcgatgat gaccggcgca tgcaggttgg cagcggtttc
    36601 caccaccact tgcatcgttt cgagattgtg aatattgaat gccggaaccg cataaccgcc
    36661 gcgctgcgcg ttgttcagca tctgctttgt cgataccacg tatattttca tatcctatcg
    36721 tttgttttcg atttcaaaat ataatgaaat tatttgtttt caaatgtcga gataacgatc
    36781 acaaaaacga caatgtgaaa attaatcaga atgacaggca aaaaaaacgg cctcccgggg
    36841 gaagccgtaa cgaagtgcgc gtgtttttat gccggatgca acgtaaacgc cttttccgtc
    36901 atacggggta gtgctgttcg tatgaggcgt atcaggcgat agtaattttg ctatcgagat
    36961 aaacgtcctg cacggcgtta atcagtttca cgccgtcagc catcgatttc ttgaacgcct
    37021 tacgtccaag aatcagcccc attccgcctg cgcgtttgtt gataaccgca gtacgcactg
    37081 catcgctgag gtcagtttca ccgcctgcag caccgccgga gtttatcaac cctgcgcggc
    37141 ccatatagca gttagctaac tgataacgca ccagatcaat cgggttttcg ctggtcaact
    37201 tgctgtacac gcgatcgtcg gtataaccgt aattaatcgc tttatagccg ccgttatttt
    37261 ccgccatttt ttgtttaacg atatctgcac ctatggtcgc cgccagatgg tttgcctgac
    37321 cggtcaggtc ggcggaaaca tggtaatcaa cgccatcttt cttaaaggcg gagttacgca
    37381 aataggccca cagcactgtc accatgccca gctcgtgcgc acgttcaaaa gccgcagaaa
    37441 tttcttcaat ctggcgacgt gactcttccg aaccaaaata gatagtcgca ccaaccgcca
    37501 ccgcgcccat gttgaaggcc tgctccacgc tggcatacag cgtttgatcg taggtattcg
    37561 ggtaacttag cgtctcgttg tgattaagtt taacgaggaa tggaatgcga tgcgcgtaac
    37621 gccgcgatac cgatgccagc acgccgtaag ttgacgccac acagttacag cccgcttcga
    37681 tcgccagttc aacaatgttt ttcgggtcaa agtagagcgg gttagcggca aatgaagctc
    37741 cggcagagtg ctcaacgccc tggtcaaccg gcagaataga aagatatcct gtgccagcca
    37801 gacgcccagt gttgtacaac gtctgcatat tacgtaacac cgctggcggg cgattattgt
    37861 caatcatcac gcggtctacg tagtcatgtc cggggagata aagctggtca gaaggaatag
    37921 tcatacaacg gtgctgtaaa aggttgtcgg cgtctttgcc aagcaactgc gcaatatctg
    37981 tcattactat gctcccgtaa attccgattg gatatcggct atggattgtc ctggcccgcc
    38041 ttttgcgggc aatcataatc ctggtcgtta caggcgagtt tttccatctt ttggatgttt
    38101 tttcagcgtt ttctgttggc tcgattcatc agaaaaaatg ttaccgcggt caagttttca
    38161 ccgcaaaggt atttaaaagg tattattaaa tggtattgtc atcgcgtacc ttacattacc
    38221 tgtcatgaag gaattaaaag atgaaaacaa cagcaaagct gtcgttcatg atgtttgttg
    38281 aatggtttat ctggggcgcg tggtttgtgc cattgtggtt gtggttaagt aaaagcggtt
    38341 ttagtgccgg agaaattggc tggtcgtatg cctgtaccgc cattgcggcg atcctgtcgc
    38401 caattctggt tggctccatc actgaccgct ttttctcggc gcagaaagtg ctggcggtat
    38461 tgatgttcgc tggtgcggtg ctgatgtatt tcgctgcgca acagaccact tttgccgggt
    38521 tcttcccgtt actgctggcc tactcactaa cctatatgcc gaccattgcg ctgactaaca
    38581 gtatcgcttt tgccaacgtg ccggatgtgg agcgtgattt cccgcgcatt cgtgtgatgg
    38641 gcactatcgg ctggattgcc tctggtctgg catgtggttt cttgccgcaa atgctggggt
    38701 atgccgatat ctcaccgacg aacatcccgc tgctgattac cgccggaagt tctgctctgc
    38761 ttggtgtgtt tgcgtttttc ctgcccgaca cgccacccaa aagcaccggc aaaatggata
    38821 ttaaagtcat gctcggcctg gatgcgctga tcctgctgcg cgataagaac ttcctcgtct
    38881 ttttcttctg ttcattcctg tttgcgatgc cactggcgtt ctattacatc tttgccaacg
    38941 gttatctgac cgaagttggc atgaaaaacg ccactggctg gatgacgctc ggccagttct
    39001 ctgaaatctt ctttatgctg gcattgccgt ttttcactaa acgctttggt atcaaaaagg
    39061 tattgttgct tggtctggtc accgctgcga tccgctatgg cttctttatt tacggtagtg
    39121 cggatgaata tttcacctac gcgttactgt tcctcggtat tttgctgcac ggcgtaagtt
    39181 acgattttta ctacgttacc gcttacatct atgtcgataa aaaagctccc gtgcatatgc
    39241 gtaccgccgc gcaggggctg atcacgctct gctgccaggg cttcggcagt ttgctcggct
    39301 atcgtcttgg cggtgtgatg atggaaaaga tgttcgctta tcaggaaccg gtaaacggac
    39361 tgactttcaa ctgggccggg atgtggactt tcggcgcggt gatgattgcc attatcgccg
    39421 tgctgttcat gatttttttc cgcgaatccg acaacgaaat tacggctatc aaggtcgatg
    39481 atcgcgatat tgcgttgaca caaggggaag ttaaatgaaa acagaacgta ttctcggtgc
    39541 tctttatggg caggcgttag gggatgcgat ggggatgccc tccgagcttt ggccgcgcag
    39601 tcgcgtcaaa gcacactttg gctggattga ccgttttctg cctgggccaa aggagaataa
    39661 cgcagcctgt tattttaacc gcgccgaatt caccgacgat acctcgatgg cgctgtgtct
    39721 ggcggatgcg ttactggaac gtgaaggcaa gatcgatccg gatctgattg ggcgtaatat
    39781 tctcggctgg gcgctgcgtt tcgacgcctt taacaaaaac gtactaggtc cgacctcgaa
    39841 gattgcgctt aacgacattc gcgacggtaa acccgttgct gaactggaaa ataacggcgt
    39901 gaccaacggc gcagcgatgc gcatctcgcc attaggttgt ttgcttccgg cgcgcgatgt
    39961 tgattccttt attgatgatg tggcgctggc ctccagcccg acccataaat ccgatctggc
    40021 ggttgcgggg gcggtagtca tcgcatgggc gatttctcgt gccattgacg gagaaagctg
    40081 gtcagcgatt gttgattcac tgccttcaat tgcgcgacat gctcaacaaa aacgcatcac
    40141 taccttcagc gcctcactgg cagcacgtct ggagattgcg ctgaaaattg tgcgcaatgc
    40201 tgacggcacc gaatccgcca gcgaacagct ttaccaggtc gttggcgcag gtaccagtac
    40261 tattgagtcc gttccgtgcg ccattgcgct ggttgaactg gcacaaaccg acccgaaccg
    40321 ttgcgccgtc ctgtgcgcta accttggcgg cgacacagac accatcggtg ctatggcgac
    40381 ggcaatttgc ggcgcgttgc atggcgttaa cgctatcgat cctgcgttaa aggcggagct
    40441 ggatgcggta aatcagcttg atttcaaccg ctatgccaca gcgctggcga aatatcgtca
    40501 acaacgggag gcggtatgag cagcgctcga ttacacacgc tgctgcctga attaaccccg
    40561 cgtcagccag ttatggtgat tggcgcggcg gtcattgatg tgatcgccga cgcttatgcc
    40621 ctcccctggc gtgggtgcga tatcgaactg aaacagcaga gcgttaacgt tggcggctgc
    40681 gcactgaata ttgccgtggc gttaaagcgc ctcggcatcg aagcgggtaa tgccttgccg
    40741 ctcggtcagg gggtgtgggc ggagattatt cgcaaccgga tggcaaaaga ggacttaatc
    40801 agcctgatcg ataacgccga aggtgataac ggctggtgtc tggcgctggt tgagccggat
    40861 ggcgaacgca cttttatgtc gttcagcggc gtggaaaatc agtggaatcg ccagtggctg
    40921 gcgcgattaa ccgttgcgcc tggcagcctg ctctattttt ccggttatca actggcctcg
    40981 ccctgcggcg aattgttagt ggaatggctg gaagagctgc aagacgtgac gccatttatc
    41041 gattttggcc cacgtattgg cgatatcccg gatgcattac tggcgcggat catggcctgt
    41101 cgacctttag tgtcactcaa tcgtcaagag gctgagattg ccgccgaacg ttttgctcta
    41161 tccgcagaga taacaacact aggcaagcaa tggcaggaga aatttgccgc gccgttgatc
    41221 gttcgtctcg ataaagaggg cgcatggtat ttcagcaacg acgcttctgg ctgcattccg
    41281 gcatttccaa cgcaagttgt agacaccatt ggggcgggcg acagtcatgc cggcggcgta
    41341 cttgccgggc tggcctctgg tctgccactg gcggatgccg tattactggg caatgcagtg
    41401 gcgtcgtggg ttgtcgggca tcggggcggt gattgtgcgc caacgcgcga ggaactactc
    41461 ctcgcacaca aaaacgtata gatcgctgcg acagtggctg atgctgtact caataggccg
    41521 ttgctgttgg tcaagcgcca cttgcttgat caccagcacc ggtattttgc tatctaactg
    41581 aatatgtgac tgaaactcgg catccggcat ccgggcgcta acgcgggaac gcgtacgttg
    41641 tgggtaaatg tgctggctgc ggaagtagtc ataaagagaa atcccgatgg catcaacatc
    41701 atgaattaaa tgtgccggaa cccacgattc ctcaatcgat accgcctctt catcgacata
    41761 gcgaatacgc ttaagcaaaa agacatcact tcccgcttcg accgccagct gctgcgcgac
    41821 ttcttcggga catttcacga cacgcttgtt aacccataac gtatcgggct ttttcccgcg
    41881 caataccacc tgctgagaaa ccccccgcgc ttctttcagc gagtattcga agatgttgtt
    41941 gatctgcgtg ccgtaaccac gcgaacgcgt caccacgccc tcttcttcca gcgactgcat
    42001 cgccttgcgc accgtaatgc gcgaaacgcc ggttaactga cttaaatcac gctcaccggg
    42061 caaaatattg ccatgctcca gcaccccgct gcgcacggca ttttttaccg tttcggcaaa
    42121 cttaatataa agcggcgtgt tatccgccgc taaaatacgc tcgttcagtt gggcaatcag
    42181 ctgggtatgc gcttgttcca tttgtctttt tcctgccggg gaatctggtg ggagtatatc
    42241 actgcttcat atttaaggcg tttctttaat cccatcaacg aatccctgca actcctccac
    42301 cgtgccatta aacacattaa aatccaccgg accattaatg ccatcgacct gtccacggtc
    42361 ggaatgctgc cagaagcgcc aggccatgcc gtcattgtcc gggcgacgtt gataatagtg
    42421 agccacccac cacggatatt cattgaaata gcccgccaga ttggtgtgat aaaaaacggc
    42481 tcctgagtaa ataattggct ttttacccgt acgtttttcg accattttta gccactgact
    42541 tacccgcttg cgtaattctt ttgccgataa ttttccccgt tcttctacgt ccagcacggc
    42601 agggaaatcg ccttgtgaga aatccaccgt ttgcagaaat aatctcgcct gaactgaagc
    42661 agataccgac ggggaaaaat aatgatacgc cccgcgcagc aggccatttt cgcggcttag
    42721 ttgccagtta cgcgaaaaat aggggtccac cagcttttcg ccttccgtcg ccttaataaa
    42781 ggcaaattgt aagcgaatac cgttatcgcg catttttgcc acccgctgcc agtcgatccg
    42841 ctcctgccag cgcgaaacat ctattccgtg aatggtgtaa ctggcaggaa tgcgaatagc
    42901 gaaagattta accgggcgat aaccgtagaa gtgaatgtag tctttaatct gaattgctgt
    42961 cgaatagaaa aagttcaccg tctgacgcgg ataaattgca acgatggaaa ttagcccaag
    43021 agcgcataat aaaacggtga atttttttcg actggtgatt ctcaactgca ttggtttcat
    43081 ccctgaatgt cagcgccaaa ggctgacaat aaccaaagca gctatagtac ggtgctcacc
    43141 accaggcgtg gaagtggtga accggaccaa taccgtgacc aacttccagc gtgtcggcct
    43201 gggctaatgc cgatgaaagc cagctttttg cctcctgtac ggtgtcagcc cagtttgtat
    43261 ggcgcgggcg cagtgcagcc aacgccgcag agagcgtaca accagtgccg tgagtgtttt
    43321 tggtcataat gcgctgtgcg gtaaaccgtt gttcaccctc gcgggtaaac agccagtccg
    43381 ggctttgctc atcatccaga tgaccacctt tcattagcac tgcgccacag cccatcgcca
    43441 acagcgatcg cccttgttcc agcatttcct gttcggtgcg cgcgtgtggc gcgtcgagca
    43501 aggcggctgc ttcgggcaag tttggcgtta ttaatgaaac ctgtggcaat aatcgactgc
    43561 gcagcgtagc aactgccgaa ggtgaaagca gcgggtcgcc gctttttgcc agcataacgg
    43621 tgtcgagtac cacgttttgg atctgataac gttgcaaccg ttctgccacc gcttcaacaa
    43681 tatcggtttc cgccaacata ccgattttag tggtgtcgat tcgcacatcg ctgaacaccg
    43741 aatcgagctg ggcggcgaca aaatcaggct caatgcgata caccgactgt acgccacggg
    43801 tattttgcgc caccagtgca gtaataactg agcaaccata agcgccaagt gccgagaagg
    43861 ttttaagatc ggcctgaatc cccgcaccac cactcggatc agtaccggca atcgtcagag
    43921 cgttaattcg tttcatgcct gcacctcctg cgtcaattgc cagagcgcat caaggaaatg
    43981 tggaacaaaa ctgcctggcc cctcgcttct ggcaactgcg cgttctccgg cttgtttcat
    44041 ccagtgacag gcagatgcga tattttccag ggtatcgcct ggtagcgcac aacaggcagc
    44101 gacaaccgcc gatagtgcac agccagttcc taccactttg gtcattaacg gatcgccacc
    44161 gtgaatgcca atgatacgat gtccatcggt aacataatcc acctcgccag tgaccatgac
    44221 gattgcgcca gtttcccgtg ccagtgtttg tgcagcgggt atcgcgttag ctgcggcatc
    44281 agtggtatcc actccccgtc caccattagc aacgccagct aatgccatga tttccgaagc
    44341 attaccacgt atggcggttg gcttatggga cagaagttcc acacaaaaac ggcggcgata
    44401 atcgagcgca ccaaccgcaa ccggatcaag cgtccagggc gtttgtgatc gtgttgcttg
    44461 ctcaacggca gcactcatcg cttgcgctcg tggctgcgtc agtgtgccaa cgttaatcag
    44521 caacgcactg gcgatagccg caaactgact ggcttcttcg gtttcgataa ccatcgctgg
    44581 cgatgcaccg agcgccagca aggtattggc agtaaaggtt tgcaccacat cgttggtcat
    44641 acagtgcaca agaggggaat gttggtgaaa aaggtgtaac gcgtgcgcag attgcgctga
    44701 actcagcagg tcgacttgca tagtttgctc ctgccataac gtgaagaagc aatgacctgg
    44761 tggtccgtga cttccctacg ctggcattat ccagatcagg tgatacgggt atttctcagc
    44821 cttcacaaag aagggcaccc cgagtcgtta ggttgcgata ataaatcgca gaggaggatg
    44881 gtaatgtcca gcgcacgcgt tgtaaacggg gaaaaacggc agttttacaa tcgcgttaat
    44941 aaattatttg atataagaat ccagcacctt cagaacgaca tccagatctt cttctcgttt
    45001 tagctcatcc ccctggtgaa cgatgtgttc cgtcagatga cctttaatca cttcccgcat
    45061 cagaccgttt accgcgccac ggatagcagc aatctgttgt aaaactgcag cgcattcatg
    45121 cggttcgtcg agcattttct tgagcgccac gacctggccc tgaatcttac tggcacgcgc
    45181 tttcagtttc tgtttatcac ggattgtatg agacatggca acacctggtt aacaagaata
    45241 tgaaaaatca tagcactatt aatctactgg ggggtagtat caggtactgg gggggagtag
    45301 aatcagattg ccgaattaat actaagaatt attatcatga ccgaatttac aacacttctt
    45361 cagcaaggaa acgcctggtt cttcatcccc agcgccatct tacttggtgc gcttcatggc
    45421 ctggaaccgg ggcactcaaa aacgatgatg gcggcgttta tcatcgccat caaaggcacc
    45481 attaaacagg cggtgatgct tggactggca gcaactattt cgcataccgc agtggtctgg
    45541 ttaattgcct ttggcgggat ggtgatcagc aaacgcttta ctgctcaatc agcagaaccg
    45601 tggctccagt tgatttccgc agtgatcatt attagcaccg cgttctggat gttctggcgt
    45661 acctggcgcg gcgaacgcaa ctggctggag aatatgcacg agcatgatca tgagcatcat
    45721 caccacgatc acgaagatca ccacgaccat ggacatcatc accatcacga acatggcgag
    45781 tatcaggatg cccatgcacg agcccatgcc aatgacatta aacgacgctt tgatggtaga
    45841 gaggtcacca actggcaaat tttgctattt ggcttaaccg gtggccttat cccctgcccg
    45901 gcagcaatta ccgtgctgtt gatttgcatt cagttgaaag ccctgacact gggcgcaaca
    45961 ctggtcgtca gtttcagcct cggcctggcg ttaacgcttg tcaccgtagg cgttggcgca
    46021 gcaatcagcg ttcagcaggt cgcaaaacgc tggagcggat ttaacactct cgctaaacgc
    46081 gctccctatt tttccagtct gttgattggc ttagttggtg tgtatatggg cgtacatggc
    46141 ttcatgggca taatgcgata actcaatcct ttaggcttgc atgttatctt cagcccagga
    46201 tgattcttaa atcagctatt tccgctgact ctctgctcgc taaggatgct tttagggcat
    46261 cctttcattt acacttttta cgaaatcatg ggatcactaa caaaatatcg cttgtcagtt
    46321 atattgtatg gcaggaaaga tatgcgactg atattacaga tccccaaagt ggagagttta
    46381 tgaccattaa aaataagatg ttgctgggtg cgcttttgct ggttaccagt gccgcctggg
    46441 ccgcaccagc caccgcgggt tcgaccaata cctcgggaat ttctaagtat gagttaagca
    46501 gtttcattgc tgactttaag catttcaaac caggtgacac cgtcccagag atgtaccgca
    46561 ccgatgagta caacattaag cagtggcagt tacgtaacct gcccgcgcct gatgccggga
    46621 cgcactggac ctatatgggt ggcgcgtacg tgttgatcag cgacaccgac ggtaaaatca
    46681 ttaaagccta cgacggtgag attttttatc atcgctaaaa aaaagccccc tcggagagag
    46741 gggggaatgc agacacctta atatttttat tattagccac ttgctcgtct tgcttgttat
    46801 tagtcgtatt tcacgttgat taatgcggtt gcctccagtg cgccagattt aactttgttt
    46861 gtatcgtaga cgtagtaact ggccgttatc ggaattgaaa ccgttgtatc tgtaagtttt
    46921 gactttgccg caatttcttt taaatttgta tatcctttac tgctgttagt gatatttaaa
    46981 agtgtgactg gcgtaccatt actttcaaga ataaatccta cgccattatc ttccgcacct
    47041 ttcaggatcg tgttgctgtt ggttaacaag tcagaacttg ataaatacac ctgcattttc
    47101 cgtgatgttt gattttgttg acctaataaa tttttacaac gcagaataat atcccctttt
    47161 ttactattta ttgttgtttt gttacctgtg gctggtaatt gtgaaacggg aatactctct
    47221 aacgtaatta ataaatcgtc cggtttgcag gttgtggtta tcgggttgta tgacaatgtc
    47281 agattaaagg aatagacacc tgcattttgt gccaatgcat ttctgtagct atttacacat
    47341 gcatcccatg acttaccacc taataaacac agtagaaatt gtactgcggc actcccgcgt
    47401 gtcgatgatg aagcactgga taatgtcgca actccattaa tcatcactga accaccgccc
    47461 cccgcagaaa cattcacttt tgcattactg gccgcaggtg tcaccgtcac cgaataacca
    47521 ctggtgaaac tggtctgatt atttgacaac gtaatgttgt ttttattggt ccattcaaaa
    47581 tgtaaattta atttaatggt atcgttataa agtccaacaa taatatccga cgggctcata
    47641 ggtgtgatag tatttgtact cacgcatgac gaatcattaa aattatgctc atatgtcgcc
    47701 atttgtgttt gagtaagtgc aaaatccccc tgaacaacca aattgtgctg aacagataac
    47761 tcactataac atgccgcata gctcacaggc ataaccatca tcattactat aaatagcatt
    47821 atgcgaattt ccatagaacc acctgactat tactggcaaa tataatttct actttcatca
    47881 atctctttac cgaaggtgat tgtgcatgaa agcccttgtt gcttatcaat tgccacatta
    47941 accgatggcg gtacttcatt ggtgcgaata aataactggc ttccctggcc gacaacacca
    48001 atattatgac catggatatc attgacttca taaccaaacg ttaatggttg cccatccgct
    48061 cttaacgctt ttataaacca tggcttgcgc tgatcggtat caaaattaac cagtacaacc
    48121 gcgccgcgat aaggggcggc aattttccgg ttgccacgta attctgcttc gctatcgctt
    48181 tgtgacacat ccaacatcag gtaattttcc cgataaggtg tcattccgtc gtataccacc
    48241 actccattac ggtttgttgt gcgatatttt tgcccattga cataagcatc tttaattcct
    48301 ggcgcattca tcacagcaaa cgtttcagaa agacggtttg ccagattaac gccacccgac
    48361 caggcgacaa tgccccctga aacactggct ccagcctgtc gataagcact cgactgacta
    48421 taactgccat tcactgtcgc aaccggcgcg ttccaggtta aattcgcccc agctgtcgtt
    48481 tcatttccct gatactgata actcaggttg accccatagt taaactgatc ccggcttcca
    48541 acggttcctg ataatcccgt attatttgag gcaaccccct gatcatcaaa cgtcgttgag
    48601 ttagacatat atatttgccg acgaggcgtc gtaacgtcat caccccaatc aaagggaatc
    48661 gatataaaaa tattaaaacg tttctcttca tgatgattct cgtcataagc atggcttgcc
    48721 gcgagggtat agcttatcct tcgcaagttg ttggaataac tcaactgata atccttactg
    48781 ctgccgctac gcccccagta atctcgccat aacgtactta aggacacaga accccagcct
    48841 tctggcaatg actgactcat attggcagag aagctatttt tacggccaaa atcgttctgg
    48901 taataatcgg caatgtcata gatatcgttt tcatcatggc gataattatc tttattgttt
    48961 gcccatacgt gatcattaaa tgtccgataa tcacgcgacg aataacgcca ggccgccaga
    49021 ccaaaacgtg tcgacgtttg gctcacaaat ttgttgtagg caatttgata actttgcccg
    49081 tcaaacacat cgccgttgtc ttgtttacta tgcgacttcg tggcatcgac ggaaatggca
    49141 ccaatgcgtg tattccagcc agtccccaaa gtgaacgcgt aataattatt cgcgaccatc
    49201 gagccaccat acagcgttaa taagttatta aaaccacgcc tggacaaaat cactttgttt
    49261 gctcgcccct tcaatatggc tacgacccgc cgcaaaatca tatttcgaca cgccgggttg
    49321 cagcatattt ggtaccgctg cataaggcac cagataggtg gttaccgaac cgtccgcctc
    49381 tttcacgctg acatcaagat ccgctccacc accggccaac tgcaaatctg taatcgcaaa
    49441 cgggccagga ggaacctctt tctgataaac cacaaaacca ttctgttcaa tagttaccag
    49501 cgcgttactc tgagcaatcc cctgcacccg tggcgtaaaa ttttgtttcg agttaggcaa
    49561 catctgcata tcacgaaaca accgcacacc gctgaagcga acagaatcaa aaatatcgct
    49621 tgatgtgtac atatcaccca cacgaagcgt gccgagaagt tgggcaaatc cacgttccag
    49681 atacagggta ttgcttttcc acacccctgg attgttattt gttttactga aactggcatc
    49741 agaatgcagt tgccacccca ataaatttaa cccgctgtta aaacgtacat atgtactctt
    49801 gctattaccc gacgctttat agtcgctgta atactgactc acataataag aggtataaaa
    49861 cgcattaata ccccgctccc agttttcagg tggtacatag ccactttcca gttcttccac
    49921 ctaggcctgc gggacactga aatcgagacg aaaaactccg atatcccagg tatagctccc
    49981 gccctgaata agttgcttaa atgttaaaca ttgcttaccg ctggcaaagc tatcggtatt
    50041 aatgcctaac cgcttgatca tttcccttga taaacatgtt tcttgcgggt tgtctttaac
    50101 aatgatctca tatttcccac gccattgctt attgacataa atatcgatgt catactgccc
    50161 cggcaagggc tgattatctt caagacgaat atttgcaacc tgctggtctt tcattccacc
    50221 tatcatgaaa tgggtatcaa aggtttcttc agctgcataa gcttcaatgc cgatcaataa
    50281 cgctacgatt gctgatgcaa gtggggtcat tctcaacata ggcagctcct gaaattaaat
    50341 tttgtcacta atataattgc catggtcatc gataatggtc agatgccagt tatttgcatt
    50401 attacttttg acattaacac tctaactttc taagggggca atcataatag tttcataatt
    50461 gactttgaca ttattagctt tgacatccga aatcgtcacc caattagctg aggcattttt
    50521 tatcaccaat ccattgccac tgcgatttac cagtaacttt ttaaaggtcg ctttatttac
    50581 cggagcaata cccgctggcc ggtaaaacaa cttaattctg ttttgcatcg caaacttcag
    50641 tgcattctta ccttc
//
LOCUS       DAZFFE010000021        48513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFFE010000021 DAZFFE010000000
VERSION     DAZFFE010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.021"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            244..396
                     /gene="mokC"
                     /locus_tag="ACHJ69_001447"
     CDS             244..396
                     /gene="mokC"
                     /locus_tag="ACHJ69_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO5014463.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(501..1631)
                     /gene="dnaJ"
                     /locus_tag="ACHJ69_001448"
     CDS             complement(501..1631)
                     /gene="dnaJ"
                     /locus_tag="ACHJ69_001448"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO5014464.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTNSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1720..3636)
                     /gene="dnaK"
                     /locus_tag="ACHJ69_001449"
     CDS             complement(1720..3636)
                     /gene="dnaK"
                     /locus_tag="ACHJ69_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO5014465.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4008..4412
                     /gene="yaaI"
                     /locus_tag="ACHJ69_001450"
     CDS             4008..4412
                     /gene="yaaI"
                     /locus_tag="ACHJ69_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO5014466.1"
                     /translation="MKSVITISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASIYFKATRSASQTLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4438..5151
                     /gene="msyB"
                     /locus_tag="ACHJ69_001451"
     CDS             4438..5151
                     /gene="msyB"
                     /locus_tag="ACHJ69_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO5014467.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPERHHRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEISTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5300..5866
                     /gene="satP"
                     /locus_tag="ACHJ69_001452"
     CDS             5300..5866
                     /gene="satP"
                     /locus_tag="ACHJ69_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO5014468.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMQKLGLTDAPNAQFLGVY
                     LGLWGIFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(6071..6658)
                     /gene="mog"
                     /locus_tag="ACHJ69_001453"
     CDS             complement(6071..6658)
                     /gene="mog"
                     /locus_tag="ACHJ69_001453"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO5014469.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARREVSE"
     gene            complement(6773..7726)
                     /gene="tal"
                     /locus_tag="ACHJ69_001454"
     CDS             complement(6773..7726)
                     /gene="tal"
                     /locus_tag="ACHJ69_001454"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO5014470.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            8005..9435
                     /gene="yaaJ"
                     /locus_tag="ACHJ69_001455"
     CDS             8005..9435
                     /gene="yaaJ"
                     /locus_tag="ACHJ69_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO5014471.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGRPAWYMARGLGMRWMGVLFAVFLLIAYAIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFAMLAITRGLHGVARLMQGFVPLMAIIWVLT
                     CLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPEIGRQLSPDAWDDVSQE"
     gene            9505..10281
                     /gene="yaaA"
                     /locus_tag="ACHJ69_001456"
     CDS             9505..10281
                     /gene="yaaA"
                     /locus_tag="ACHJ69_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO5014472.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISSLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10331..10627)
                     /locus_tag="ACHJ69_001457"
     CDS             complement(10331..10627)
                     /locus_tag="ACHJ69_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO5014473.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPYGPPPSPRHNKHNDHRGDHRPGPDKHHR"
     gene            complement(10841..12127)
                     /gene="thrC"
                     /locus_tag="ACHJ69_001458"
     CDS             complement(10841..12127)
                     /gene="thrC"
                     /locus_tag="ACHJ69_001458"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO5014474.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12128..13060)
                     /gene="thrB"
                     /locus_tag="ACHJ69_001459"
     CDS             complement(12128..13060)
                     /gene="thrB"
                     /locus_tag="ACHJ69_001459"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO5014475.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13062..15524)
                     /gene="thrA"
                     /locus_tag="ACHJ69_001460"
     CDS             complement(13062..15524)
                     /gene="thrA"
                     /locus_tag="ACHJ69_001460"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO5014476.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15550..15669)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15605..15670)
                     /gene="thrL"
                     /locus_tag="ACHJ69_001461"
     CDS             complement(15605..15670)
                     /gene="thrL"
                     /locus_tag="ACHJ69_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO5014477.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15884..16570)
                     /gene="yjtD"
                     /locus_tag="ACHJ69_001462"
     CDS             complement(15884..16570)
                     /gene="yjtD"
                     /locus_tag="ACHJ69_001462"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO5014478.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPVA
                     RWVAHGSGDIIDNIKVFKTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERAMTLLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16970..17110)
                     /gene="yjjY"
                     /locus_tag="ACHJ69_001463"
     CDS             complement(16970..17110)
                     /gene="yjjY"
                     /locus_tag="ACHJ69_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO5014479.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AI"
     gene            17206..17922
                     /gene="arcA"
                     /locus_tag="ACHJ69_001464"
     CDS             17206..17922
                     /gene="arcA"
                     /locus_tag="ACHJ69_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO5014480.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17982..19334)
                     /gene="creD"
                     /locus_tag="ACHJ69_001465"
     CDS             complement(17982..19334)
                     /gene="creD"
                     /locus_tag="ACHJ69_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO5014481.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDV
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNVALNLSGTGDLSVVPGGRYS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19392..20816)
                     /gene="creC"
                     /locus_tag="ACHJ69_001466"
     CDS             complement(19392..20816)
                     /gene="creC"
                     /locus_tag="ACHJ69_001466"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO5014482.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILCEGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVDQEYVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20816..21505)
                     /gene="creB"
                     /locus_tag="ACHJ69_001467"
     CDS             complement(20816..21505)
                     /gene="creB"
                     /locus_tag="ACHJ69_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO5014483.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFDVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARNEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21518..21991)
                     /gene="creA"
                     /locus_tag="ACHJ69_001468"
     CDS             complement(21518..21991)
                     /gene="creA"
                     /locus_tag="ACHJ69_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO5014484.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22202..23071
                     /gene="robA"
                     /locus_tag="ACHJ69_001469"
     CDS             22202..23071
                     /gene="robA"
                     /locus_tag="ACHJ69_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO5014485.1"
                     /translation="MDQAGIIRDLLIWLEGHLDHPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNSPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(23068..23715)
                     /gene="gpmB"
                     /locus_tag="ACHJ69_001470"
     CDS             complement(23068..23715)
                     /gene="gpmB"
                     /locus_tag="ACHJ69_001470"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO5014486.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLETRNIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23767..24294
                     /gene="yjjX"
                     /locus_tag="ACHJ69_001471"
     CDS             23767..24294
                     /gene="yjjX"
                     /locus_tag="ACHJ69_001471"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO5014487.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEVLGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRTSVYHQAVIL
                     ALSPFHNAVYQPLQA"
     gene            complement(24367..24693)
                     /gene="trpR"
                     /locus_tag="ACHJ69_001472"
     CDS             complement(24367..24693)
                     /gene="trpR"
                     /locus_tag="ACHJ69_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO5014488.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLED
                     VLLKSD"
     gene            complement(24783..26720)
                     /gene="sltY"
                     /locus_tag="ACHJ69_001473"
     CDS             complement(24783..26720)
                     /gene="sltY"
                     /locus_tag="ACHJ69_001473"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO5014489.1"
                     /translation="MEKAKHVTWRLLAVGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVSANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            26927..28594
                     /gene="ettA"
                     /locus_tag="ACHJ69_001474"
     CDS             26927..28594
                     /gene="ettA"
                     /locus_tag="ACHJ69_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO5014490.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28650..28934)
                     /gene="nadS"
                     /locus_tag="ACHJ69_001475"
     CDS             complement(28650..28934)
                     /gene="nadS"
                     /locus_tag="ACHJ69_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO5014491.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKEPARVTRYEIADVKAIREQLNV
                     SQSEMAKALGTSVDTIKSWESKRRNPTGLAAKVLATIKENPAFFRELASH"
     gene            complement(28936..29268)
                     /locus_tag="ACHJ69_001476"
     CDS             complement(28936..29268)
                     /locus_tag="ACHJ69_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5014492.1"
                     /translation="MGKAIIFIETPMFTRQIKQIATDDELKELQKELIGTPDKGDLIQ
                     QTGGLRKVRMAAGSQGKSGSVRIIYFLATEEIIYLIMAYPKNAKDSLTDTEKAQLKKL
                     TKLLKGEI"
     gene            complement(29360..30592)
                     /gene="nadR"
                     /locus_tag="ACHJ69_001477"
     CDS             complement(29360..30592)
                     /gene="nadR"
                     /locus_tag="ACHJ69_001477"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO5014493.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRYLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30613..31995)
                     /gene="radA"
                     /locus_tag="ACHJ69_001478"
     CDS             complement(30613..31995)
                     /gene="radA"
                     /locus_tag="ACHJ69_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO5014494.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(32044..33012)
                     /gene="serB"
                     /locus_tag="ACHJ69_001479"
     CDS             complement(32044..33012)
                     /gene="serB"
                     /locus_tag="ACHJ69_001479"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO5014495.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGERVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            33118..33762
                     /gene="ytjB"
                     /locus_tag="ACHJ69_001480"
     CDS             33118..33762
                     /gene="ytjB"
                     /locus_tag="ACHJ69_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO5014496.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33790..34806
                     /gene="lplA"
                     /locus_tag="ACHJ69_001481"
     CDS             33790..34806
                     /gene="lplA"
                     /locus_tag="ACHJ69_001481"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO5014497.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNSDLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            34838..35101
                     /locus_tag="ACHJ69_001482"
     CDS             34838..35101
                     /locus_tag="ACHJ69_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5014498.1"
                     /translation="MIDLENQEREIINLMFSQDISWLTAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAGIYGCPPELLICPSWMTAEHK"
     gene            complement(35262..35981)
                     /gene="deoD"
                     /locus_tag="ACHJ69_001483"
     CDS             complement(35262..35981)
                     /gene="deoD"
                     /locus_tag="ACHJ69_001483"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO5014499.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(36038..37261)
                     /gene="deoB"
                     /locus_tag="ACHJ69_001484"
     CDS             complement(36038..37261)
                     /gene="deoB"
                     /locus_tag="ACHJ69_001484"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO5014500.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(37313..38635)
                     /gene="deoA"
                     /locus_tag="ACHJ69_001485"
     CDS             complement(37313..38635)
                     /gene="deoA"
                     /locus_tag="ACHJ69_001485"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO5014501.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(38713..39492)
                     /gene="deoC"
                     /locus_tag="ACHJ69_001486"
     CDS             complement(38713..39492)
                     /gene="deoC"
                     /locus_tag="ACHJ69_001486"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO5014502.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIDIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKKACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            39750..41300
                     /gene="yjjI"
                     /locus_tag="ACHJ69_001487"
     CDS             39750..41300
                     /gene="yjjI"
                     /locus_tag="ACHJ69_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO5014503.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPAL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLAHGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPDITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLEDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            41272..42135
                     /gene="yjjW"
                     /locus_tag="ACHJ69_001488"
     CDS             41272..42135
                     /gene="yjjW"
                     /locus_tag="ACHJ69_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO5014504.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVAWSAAACEQCDTCLKMCPQHATPMVQSMSVDEV
                     LNHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLCHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAALIKGLGDVPVRLNAFHAHGVYGEAQSWPSATPEDVEQLADALRERG
                     VSRLIFPALYL"
     gene            complement(42352..43131)
                     /gene="yjjV"
                     /locus_tag="ACHJ69_001489"
     CDS             complement(42352..43131)
                     /gene="yjjV"
                     /locus_tag="ACHJ69_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5014505.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERHPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYTLFNVP"
     gene            complement(43128..44201)
                     /gene="yjjU"
                     /locus_tag="ACHJ69_001490"
     CDS             complement(43128..44201)
                     /gene="yjjU"
                     /locus_tag="ACHJ69_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO5014506.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYREIQQFIEKPPGQLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLSRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(44323..44484)
                     /gene="ytjA"
                     /locus_tag="ACHJ69_001491"
     CDS             complement(44323..44484)
                     /gene="ytjA"
                     /locus_tag="ACHJ69_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO5014507.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(44611..45216)
                     /gene="osmY"
                     /locus_tag="ACHJ69_001492"
     CDS             complement(44611..45216)
                     /gene="osmY"
                     /locus_tag="ACHJ69_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO5014508.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(45609..47195)
                     /gene="prfC"
                     /locus_tag="ACHJ69_001493"
     CDS             complement(45609..47195)
                     /gene="prfC"
                     /locus_tag="ACHJ69_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO5014509.1"
                     /translation="MTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGT
                     VKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTL
                     TAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENE
                     LKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAV
                     GEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAP
                     APMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVR
                     TAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNF
                     APELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVA
                     RLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSMV
                     NLRLAQERYPDVQFHQTREH"
     gene            47415..47663
                     /locus_tag="ACHJ69_001494"
     CDS             47415..47663
                     /locus_tag="ACHJ69_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO5014510.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKLYDDVDVIVKS
                     ASNDGLTILRSVDKESAQKFVQETLQEVWETADDWFIR"
     gene            complement(47700..47873)
                     /locus_tag="ACHJ69_001495"
     CDS             complement(47700..47873)
                     /locus_tag="ACHJ69_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014511.1"
                     /translation="MLPDLQRLQVIQGQMMNKDKCRMYGRKRYTAGAMCFMLFVWLGG
                     VVTLCAVAAIIMQ"
     gene            complement(47878..48351)
                     /locus_tag="ACHJ69_001496"
     CDS             complement(47878..48351)
                     /locus_tag="ACHJ69_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014512.1"
                     /translation="MKVILATRNRYLEYGLQALLKEHSVILAREFFLPENRRYIPDFD
                     ESWLIICDTLLGRLMRCMFQGRHFLQLGAESLRDGEQISDAIRNGVWTYNSVARPLTM
                     SEMVVMFGYVYRQSRPCRLASEMGIHTKTVNTFLYTGMAKNGLYGVSVKHLACAE"
BASE COUNT        11524 a        13106 c        12044 g        11839 t
ORIGIN      
        1 tgtattgaaa tatttttccg caaaaatgcg aggcgtcttc agaaaaagaa ttgataaaac
       61 agaagtgaat taaaagcgca gtataatagc ggcgggtgct tgaggctgtc tgtctcaggc
      121 attagctgaa cggcagatag agaaaagccc cgagtgatat tttaccatca acccgaggcc
      181 tcctatatgc agaacacatg tagagtgcct cttactgacc gaaaggtcaa ggagaagaga
      241 gcaatgaagc agcataaggc gatgattgtc gccctgatcg tcatctgtat caccgccgta
      301 gtggcggcgc tggtaacgag aaaagacctc tgtgaggttc acatccgaac tggccagacg
      361 gaggttgctg ttttcacggc ttacgaatcc gagtaagagc aacggcgggg agtgatcccc
      421 gccactttac aggtgctcgc atatcttcaa cgcaccctgg tttttaccct ggcctgccca
      481 cgggcaggct tttggggagg ttagcgagtc aggtcgtcaa aaaacttctt cacgccatca
      541 aagaagctct ttgaacgcgg gctgttgtgc tcgccggttg ggccaccaaa gctttcttgc
      601 agctcttgca gcagctgttt ctgcttctcg ttcaaaccta ccggtgtttc gacaacaacg
      661 cggcatagca aatcaccctg tgcgccaccg cggacagact tgacgccttt accgcgcata
      721 cggaacagct taccggtctg ggtttcgcca ggcactttca gtttgacgcg accatcaagg
      781 gtcggtactt cgatttcacc acccagcgcc gccatagcga agttgatcgg gacttcgcaa
      841 tacaggttgt tgccttcacg ctcgaaaatc gggtgctgtt taacctgaac ctgaacgtac
      901 agatcgcctg ccggtgcgcc gtgttcaccc gcttcacctt cgcccgcaag acggatgcgg
      961 tctccagtgt ccacccctgc cgggatttta acggacagcg ttttgctgcg ctcaacacga
     1021 ccatgaccat gacatttgtt gcacggatct ttgatcagcg taccgcggcc ctgacagtgt
     1081 ggacaggtct gctgcacggc aaagaaaccc tggcgcatct gcacctggcc agaaccatga
     1141 caggtcggac aggtctgcgg ctgtgtacct ggttttgcac cgctaccgtg gcaaacgtca
     1201 cactcttcca gagtcggaat gcggatctct ttggtcacgc cacgtacagc ttcttcgagg
     1261 gtgagctcca tgttatagcg taaatcagca ccgcgcgccg cacgttgacg accacgtccg
     1321 ccgccaaaaa tatcgccgaa aacgtcacca aaaatatcgc tgaagtctgc gccgccgcca
     1381 aaaccgccgc cgcccatgcc gccttgctca aacgcagcat gaccatactg atcgtatgcc
     1441 gcacgttttt gcgagttggt cagaacttca taagcttcct tgatctcttt aaatttcgcc
     1501 tcggcctctt tgtcaccctg gttacggtcc gggtggtatt tcatggccag gcgtttgtag
     1561 gcctttttga tttcacgctc ttccgctgtt ttggaaacgc ctaaaatctc gtaataatct
     1621 tgcttagcca tcttttttga tttgccccta gatgaatgca cgggcggaga ggaaattccc
     1681 cttcgcccgt gtcagtataa ttacccgttt atagggcgat tattttttgt ctttgacttc
     1741 ttcaaattca gcgtcgacaa catcgtcatc tttcgcgttg tttgcagaag catcagcacc
     1801 ggcagtctgc tgctgggcat gttgctgctg ggcgatttcc atcagtttct gggaaacctg
     1861 tgccagttcc tgcattttcg cttcgatagc ggctttgtct tcacctttca gagcagtttc
     1921 cagtgcagtc agcgcagact cgatagcagt tttgtcgtca gccggcagtt tgtcgcctgc
     1981 ttcttcaacc tgcttacggg tgctgtgcag cagatggtcg ccctggttgc gagtctgtac
     2041 cagctcttca aacttacggt cagcttcggc gttagcttct gcgtcgcgta ccattttctg
     2101 gatttcatct tcgttcagac cagaagaagc cttgatagtg atcttctgct ctttaccgct
     2161 gtttttgtct ttcgcggaaa cgtgcaggat accgtcagca tcgatatcga aggtaacttc
     2221 gatctgcggc atgccgcgcg gtgccgggtt gataccatcc aggttgaact gacccagaga
     2281 tttgttatca gccgcacgtt tacgttcacc ctgcagcaca tggatggtta ccgcagactg
     2341 gttgtcttca gcggtagaga acacctggct gtgcttggtc gggatagtgg tgtttttcgc
     2401 gatcagcgtg gtcatcacac cgcccatggt ttcgataccc agagacagcg gggtaacgtc
     2461 cagcagcagt acgtctttta cgtcaccagt cagaacacca ccctgaacag cagcaccgat
     2521 ggctacagct tcgtccgggt taacatcttt acgcggctct ttaccaaaga attcagcaac
     2581 tttcttctga accattggca tacgagtctg accaccaacg aggataacgt cgtcgatatc
     2641 agatacggac aggccagcgt cctgcagtgc aactttcagc ggctcaatgg aacggtttac
     2701 cagatcttca accaagcttt ccagtttcgc acgagtcact ttgatgttca tgtgtttcgg
     2761 accggtcgcg tctgcagtga tgtacggcag gttaacgtcg gtctgctgag cggaagacag
     2821 ttcgattttc gctttttctg ccgcttcttt caggcgctgc attgccagcg gatcgttgcg
     2881 caggtcaatg ccctgatctt tcttgaattc ttcaaccaga tagttgatca gacgactgtc
     2941 gaagtcttca ccacccaggt gggtatcacc gttggttgcc agaacttcga aggttttttc
     3001 gccgtcaact tcgtcgattt cgataatgga aatatcgaaa gtaccaccac ccaggtcata
     3061 aaccgcgata gtacggttgc cagtaccttt gtccagaccg taagccagcg cagctgcggt
     3121 cggttcgttg atgatacgtt ttacttccag accagcgata cggcctgcgt ctttggttgc
     3181 ctgacgctga gcatcgttaa agtatgccgg tacggtaata acagcttcag ttaccggttc
     3241 ccccaggtaa tcttcagcgg ttttcttcat ttttttcagc acttcagcag agatctgcgg
     3301 cggtgccatt ttctggcctt taacttcgac ccatgcgtcg ccgttatcag cagcaataat
     3361 tttgaacggc atgatggaaa catcacgctg tacttcttcg tcctggaagc ggcggccaat
     3421 caggcgttta atcgcaaaca gggtgttttg cgggttcgtc actgcctgac gtttagccgg
     3481 ctgaccaacc agagtttcac catcctgggt ataggcaatg atagaaggcg tggtgcgatc
     3541 gccttcggcg ttctccagta cacgaggagt ggtgccatcc ataatcgcta cacaagagtt
     3601 ggtagtaccc aggtcgatac caattatttt acccatctaa acgtctccac tatatattcg
     3661 gtcatcatgt ggttgtgagt ctgtaatagg ggcgaaacgt ctggtttcaa ctgcccaatt
     3721 tcattttttt gcagactcac tcactgcggt tgactactaa atggggtcgt aacccacgtc
     3781 atcaaggggg agatgcaaaa attttttgtg cttttgcgca gaaatttcgc gatatggcag
     3841 gacaagacgt gaaactgaca acaattttat tcatataaag aatgaatcgc agccaaggca
     3901 ttcatcaaaa aattgtaata aaaagaaaat attacgtacc tgaaacttct ctttatcagc
     3961 agtaaactag tttcatcccc ttacccctcc ccaccaagag aatccttatg aaatccgtta
     4021 ttacgatttc cgccagcctg gcgattagcc tgatgctgtg ctgcacggcg caggcaaacg
     4081 accataaaat cctcggcgtc attgcaatgc cgcgaaacga aaccaacgat ctggcgctga
     4141 aacttcctgt ttgtcgcatt gttaaacgca tacaactctc agccgaccat ggcgatttgc
     4201 agttaagcgg tgcatcgatt tatttcaaag ccacccgaag cgccagtcag accctgaata
     4261 ttccttcaga aataaaagaa gggcaaacca ctgactggat caacattaac agcgataacg
     4321 acaacaaacg ctgcgtctca aaaatcacct tttccggtca tacagtgaac tcatcggata
     4381 tggccacgct gaaaattatc ggcgacgact aacgccagaa tatagaaacc acaaaaaatg
     4441 aatgttaatt acctgaatga ttcagatctg gattttctcc agcattgtag tgaggaacag
     4501 ttggcaaatt tcgcccggct gctcacccat aatgaaaaag gcaaaactcg cctctccagc
     4561 gtactgatgc gcaacgaact gtttaaatcg atggaaggac atcccgagcg acaccaccgc
     4621 aactggcagc tgattgccgg agaattacag cattttggtg gcgatagtat cgccaacaaa
     4681 ctgcgcggac acggtaaatt gtatcgcgcc attttgctcg atgtttcaaa gcgattgaag
     4741 ctgaaagccg acaaagagat atctacgttt gaaattgagc aacagttact ggaacaattt
     4801 ctgcgtaata cctggaagaa aatggacgag gaacataagc aggagtttct gcacgcggtc
     4861 gatgccaggg tgaatgagct ggaagagctg ctgccgctgc tcatgaaaga caaattattg
     4921 gcaaaaggtg tgtcgcattt gctttccagc caattgaccc gcattttacg cacccacgca
     4981 gcaatgagcg tgcttgggca tggtttgctg cgcggcgcag gtctgggagg ccctgtgggc
     5041 gcagcattaa atggggttaa agcggtcagc ggcagtgcct atcgcgtgac aattccagcc
     5101 gtactgcaaa tcgcctgcct gcgccggatg gttagcgcca ctcaggtctg ataccggaaa
     5161 ccggggaaaa ttttcatttt cccctcaaaa gatcgtagac actgccccac tggctgatta
     5221 ttatgccgcg ccctgaaaac actacagtta ttcagggaaa ttatttcacc attcattcga
     5281 tgatgatttt tgaggaatta tgggcaacac taagttggct aatccggcac cgctgggcct
     5341 gatgggcttc ggcatgacca ccattctgct taacctgcac aacgtgggtt attttgctct
     5401 ggacggtatt attcttgcca tgggcatttt ctacggcggc atcgcgcaaa tttttgccgg
     5461 tctgctggag tacaaaaaag gcaacacttt cggtttaacc gcattcacct cttacggttc
     5521 tttctggctg acgctggttg caattctgct gatgcagaaa ctggggctga ccgatgcgcc
     5581 aaatgcacag ttccttggtg tctacctggg tctgtggggc atatttacgc tgtttatgtt
     5641 cttcggcacg ctgaaaggcg cacgcgttct gcaattcgtt ttctttagcc tgaccgtgct
     5701 gtttgccctg ctggcgatcg gtaacattgc cggtaacgcc gcgatcatcc actttgccgg
     5761 ctggattggt cttatctgcg gtgccagcgc aatctatctg gcgatgggtg aagtgctgaa
     5821 cgagcagttt ggtcgcaccg ttctgccgat tggtgagtct cactaattgt taagctcccc
     5881 ccgctcgaag ggataagatt gttaacaacg tgcgctttgt ttatgccgga tgcggcgtga
     5941 acgccttatt cggcctacaa aatcgtgcaa attcaatata ttgcaagaag atcgtaggcc
     6001 tgataagcgt agcgcatcag gcagttttgc gtttgtcatc agcctgatcc ccccgcctgg
     6061 ggggattttt ttattcgcta acttcgcgtc ttgcactctt cggtctgaat gctgcaacca
     6121 cttccggtgc cgtttcaacg tatggccctt ccagcaactg aatgcagtac ggtacgctgg
     6181 caaaaatacc gtgcaccaca acgttaccct cagcgtcctt cacaccttcc agcgtctctt
     6241 taatagactt cggttgaccg ggtaagttaa ggatcagcgc ctgtttgcga atcaccccca
     6301 cctgacgcga aaggatcgca gttggtacaa aatgcaggct gatctggcgc atctgttcac
     6361 caaagcctgg catctcgcgg tccgctactg ccagcgtcgc atcgggcgtt acgtcacgac
     6421 gcgccgggcc agttccgccc gtggtgagca ccagatggca actcatttca tccaccagct
     6481 cacacaacgt ttgctcgatg atcgcctgct catcggggat taagcgggtt tccagttcaa
     6541 acggcgtggt tagcgccgat gtcagccatt cttccagcgc agggatgcct ttatcctgat
     6601 aaacgccgct ggatgcgcga tcagagatgg aaactaagcc aatacgtaaa gtattcatca
     6661 agatttttcc gtttaaacag tctcgttaaa cagaatgata cactgcgaag ggagtgacag
     6721 acaggtttca gaagaggtag cgtgaccgac ttcccggtca cgctaagtat gattacagca
     6781 gatcgccgat cattttttcc agtttttcct ggtcaacagc aaacttacgg ataccttccg
     6841 ccagtttatc tactgccatt ggatcctggt tgtgctgcca caggaactcg gactcagtga
     6901 tacgcgccgg acgcgctttc acttcaccag tgtaagacag tttacgttcg atagcccctt
     6961 cgctctccgc cagctctttc agcagtgccg gtgcgatggt cagacggtcg cagcctgcca
     7021 gttccagaat ttcgccgatg ttacggaagc ttgcgcccat aaccacggtt tcataaccat
     7081 gctctttgta gtactggtag atttcagata cagaaaccac gcccggatct tctgccggag
     7141 cgtactcttt cttatcggta ttcgctttgt accagtcaag aatacggcca acaaacggcg
     7201 agatcaggaa cacgcccgct tccgcacaag cacgagcctg agcgaaggag aacagcaggg
     7261 tcaggttaca gttgatgcct tctttttcca gctgttccgc agcacggata ccctgccagg
     7321 tagaagccag tttgatcaga atacgatcgt tgctaatacc tgcatcgttg tagagtttga
     7381 tcaggcgttt tgctttcgca attgacgctt cggtgtcata ggaaagacgc gcatcaactt
     7441 cagttgagat acggcccgga accagtttca ggatttccag accaatattt acggccagtt
     7501 tgtcggtcgc gtccacgatc tgctgcgcgc gatcgttgct ctgctgtttc gcccaggcga
     7561 cagcatcatc aatcaactta cggtattccg gaatctgcgc tgcgttaaga atgagagaag
     7621 ggttggttgt ggcatcctgc ggttgataca gcttcattgc cgcgatgtcc ccagtgtcgg
     7681 ccactacggt ggtgtactga cgaagggagg tcaatttgtc cgtcatgata gtatttctct
     7741 ttaaacagct tgttaggggg atgtaaccgg tctgccctga tgatatcacg acgcaacgtc
     7801 agcgcaaccg ccgacaagac ggttatcgcc aggcgagact gtttcggatt tctgacactg
     7861 cgttgatatc gcccgccatt tttatacaaa acctcatgta tgctacgcag aagttatcaa
     7921 gtacctcgta gcgtatatac ttcttaaaca attggtaacg tttacacagg aaagtcatcg
     7981 cgaccggcaa taagagggat atgcatgcca gattttttct cctttattaa cagcgtcctt
     8041 tggggatcgg taatgattta cctgctcttc ggcgcaggtt gttggttcac ttttcgcacc
     8101 ggatttgtgc agtttcgcta catccgacag tttggcaaaa gtcttaaaaa tagcattcat
     8161 ccacagccag gcggtttaac ctcatttcag tcattgtgta ccagtcttgc agcgcgcgtg
     8221 ggtagcggca acctggccgg cgttgcgctg gcaattaccg ccggtggacc tggagccgtc
     8281 ttctggatgt gggttgccgc atttatcggc atggcgacct cgtttgccga atgttccctc
     8341 gcacagcttt ataaagaacg tgatgccaat gggcagtttc gtggcagacc ggcatggtat
     8401 atggcgcgcg ggctggggat gcgctggatg ggcgttctgt tcgccgtctt tttgctcatc
     8461 gcctacgcca taattttcag cggagttcag gcgaacgccg ttgcgcgagc cctgagtttt
     8521 tcttttgatt ttcccccgct ggtgacaggt attattctcg ctgtctttgc tatgctggcg
     8581 atcactcgcg gtcttcatgg cgtcgcccgg ctcatgcagg ggtttgtccc gttgatggcg
     8641 ataatctggg tactgacctg cctggtgatt tgcgtaatga atatcgggca acttccccac
     8701 gtcatttggt ctatttttga gagtgctttt ggctggcagg aagctgcagg cggcgcggcg
     8761 ggatatacct taagccaggc gattactaac ggttttcagc gcagtatgtt ttccaatgag
     8821 gcgggaatgg ggtcgacgcc aaacgcggca gcggcagctg cgtcctggcc tccgcatccg
     8881 gcagcgcaag gaattgtcca gatgattggc atttttatcg acaccctggt catctgcacg
     8941 gcaagcgcca tgctgatatt actggcgggt aacggcacaa cctacatgcc gctggaaggt
     9001 attcagctta tccagaaggc gatgcgggtg ctaatgggtt cctggggtgc ggagtttgtt
     9061 accctcgtgg ttattctgtt tgccttcagc tccatcgttg ccaactacat ttatgccgaa
     9121 aacaatctct tctttttacg cctgaacaac cctaaagcga tctggtgtct gcggatctgc
     9181 accttcgcaa cggtcatcgg cggcaccttg ctaagtcttc cgctgatgtg gcaactggca
     9241 gatatcatta tggcctgcat ggctattacc aatttgaccg ccatattact gctctcgcct
     9301 gtggttcata ccattgccag tgattatcta cgccagcgta aactcggcgt gcgcccggtg
     9361 tttgatccgt tgcgttatcc ggagatcggt cgccagcttt ctccagacgc gtgggatgat
     9421 gtttcgcagg agtaatcaca actatcgatc aactcattct ctttttttgc taaagtcggc
     9481 ataaatttcc tgcaaggact ggatatgctg attcttattt cacctgcgaa aacgcttgat
     9541 taccaaagcc cgttgaccac cacgcgctat acgctgccgg agctgttaga caactcccag
     9601 cagctgatcc atgaagcgcg gaaactgacg cccccgcaga ttagctccct gatgcgcatc
     9661 agcgacaaac tggcgggtat caacgccgct cgctttcatg actggcagcc agatttcaca
     9721 ccggagaatg cccgccaggc aattctggcg tttaaaggtg atgtctacac tggcttgcag
     9781 gccgaaacct tcagcgaaga cgatttcgat tttgcccaac agcatttgcg aatgctttcc
     9841 ggcttgtatg gcgtactgcg cccgctcgat ttaatgcagc cttatcgtct ggaaatgggg
     9901 atacgtcttg agaatgcccg agggaaagat ctgtatcaat tctggggaga tatcatcacc
     9961 aacaagctga acgaggcgct cgcagcacaa ggcgataatg tagtgataaa tctggcgtca
    10021 gatgagtatt ttaaatcggt gaagccgaag aaattgaatg ccgagattat caaaccagtg
    10081 ttcctcgatg agaagaacgg caaatttaag ataatcagct tctacgctaa gaaagcacgc
    10141 ggtctgatga gtcgtttcat tattgaaaat cggctgacca aaccagagca actgactggt
    10201 tttaatagcg aaggttactt ctttgatgaa gcttcctcca gcaatggcga actggtgttt
    10261 aaacgctacg aacaacgtta agtttgtagt caatgccgga taatgccgga tggcaatccg
    10321 gcattgatat ttagcgatga tgtttgtcag gccccggacg atgatcgcca cgatgatcat
    10381 tgtgcttgtt atggcgcggc gatggcggcg gtccatatgg gtgccagcga ttgcctcgcc
    10441 attcataatg ttgtttccac cagccgtggt cgcgccagtg accgccatcc cagtaatagc
    10501 cacgattatc acgatcgcct atctgtaatt ttactgacgg gactagcgta atttccgcag
    10561 cctgcgctgc catgggagcg accagaacca gggaaagtgc gagtacgata gattgcatct
    10621 ttttcacagg ttactccttt tatcattgcc cgttatgggt cgatatccgg agattacgcg
    10681 atgagaacgg cagttgttat tctccgcaat cctaatctct aagttaccgc atttattgag
    10741 aatttctcct ttttccctgt cgtttttctc catattttct tcataaaaaa gccgggctgc
    10801 ataaaagcaa acccggcctg attgagataa tgaatagacg ttactgatga ttcatcatca
    10861 atttacgcaa cgcagcaaaa tcggcgggca ggttatgcga aagcaagggt aaatcagcac
    10921 gttctgccag ctcttttggc agatccaacg tttcaccgag aatcgcttcc acgctctctt
    10981 taaatttcgc cggatgcgcg gtgccgagga acaagccata ttcgcctgga ttcaactggt
    11041 cacgcagcgc acgataagct acggcagcgt gcggctccga ggtatagccc agttctttta
    11101 actcacgcat tgtctgttgc gtggtttcat catccacggc tgcatacccc agctctttca
    11161 gttgccagat tttgcggcgg aacaactctt ccacacgcgg ccagttgttt ggctggctaa
    11221 catccatcgc attggataac gtcgcctgag tcgctttggg tgaccactga ccgtcgtgca
    11281 ggaaacgtgg tacggtatcg ttcacgttgg tcgcagcaat aaaacgtttt accggcagac
    11341 cgagtgactt cgccagcaga cctgccgtta aatcaccgaa gtttccactc ggtaccgaga
    11401 caaccaactg gttacgtgct tcttgcggca actgtgcgac agcctcaaag taataacaaa
    11461 tctgcgccag caagcgactg atgttgatgg agttagcaga attcagcccc agcgccactt
    11521 tcagttcttc atcatcaaac gcctgcttca ccagcgcctg acaggcatcg aaatcgccgt
    11581 cgatggcaac agtttcgata ttgccgccca atgtacagaa cagtttttct tgcagtggac
    11641 tgattttgcc tcgtggatag aggataacca ctttcacatt cggtaaaccg tagaaagcat
    11701 gagccactgc cgctccagta tcaccagatg tcgcggtcag aatggtcact ggcttatcgc
    11761 ccgcaatatg ggtcagcatt tgtgccataa agcgaccgcc gaaatcttta aatgccagcg
    11821 ttggcccgtg aaacaactcc agacaaccga catcgctttc aacattggcg accggagccg
    11881 ggaaggcaaa cgccgcgcgt acgcgctctt ccaggatttc ctgcgggatt tcatcaccaa
    11941 taaacgccga gaggatcttc gcactgcggg tgacaaaatc cagcttcagc atctcatcaa
    12001 tttcagtcag gctgaattcc ggcaggtcgt gcgggaaaaa cagcccctga tttttgccca
    12061 acccctgggt tacggcttgc gcaaagctga cctgctcatt gtgatctttc agattgtaga
    12121 gtttcattta gttttccagt actcgtgcgc ccgccgtatc cagccggcaa atatgaacaa
    12181 aaccttcctg attttgcagg tagttcttac ccaaccagtc ggcaacgcgc tgggcggtat
    12241 ccggcttgtc acacagagcg aacaaagtcg ggccggagcc ggagataccg ctcgctaccg
    12301 cgccgatttc cgcaaccgcc tgccgcgcct gccggaagcc tggcagtaac cgttcacggt
    12361 agggttcagc gataacatct ttcatcagct tcgcggcaag ctcaggctga cgggaatagc
    12421 aggcgtgaat gaagcctgcc agatgtcgcc cgtgcgcaat gcaatcctgg cggcgatact
    12481 gcgccggtaa aatagccctg gcttctgccg tcgagacttt aatccccgga tacgccagca
    12541 cccacagcca ctcatcaaac cctggcactt gctgactgat gatgtcgttt tcttcaatca
    12601 tcaactgcat accaccaaga aaacacggtg ccacgttgtc gtaatgaatg ctgccggaga
    12661 tacgcccttc caactcgccc atcaaagcca gcaaacgagt gtcattaagc ggcttgccgc
    12721 agtgttcatt catcgccatc agcgccgcga ccactgaaca ggcgctggag cctaagcccg
    12781 aaccgatcgg catattcttt tccagagtca tcgccactgg aatttgcttg ccaagctcct
    12841 ggcaaaaacg ctcccagcac tgataaacga tattttcccg tggctctgac ggcagcttat
    12901 cggcaaagcg tccgaggttg ttgagactga atgtctctgc cgcctcaacc gtgactacat
    12961 ctccgagcaa tgcaccatca acaggtgtca ccgccgcccc gagcacatca aacccgacgc
    13021 tcatattggc actggaagcc ggggcataaa ctttaaccat gtcagactcc taacttccat
    13081 gagagggtac gtagcagatc ggcaaagaca ccggcagctg taacgtcatt gcccgcacca
    13141 tatccgcgca gtaccaacgg cagcggctga taatagtggc tatagaaggc cagggcgttt
    13201 tcgccatttt tcactttgaa cagcggatca ttaccatcca cttcggcaat cttcacgcgg
    13261 cagacgccat cttcatcaat attgccaaca tagcgcaaaa cttttccttc atcacgggcc
    13321 ttcgccacac gcgcggcaaa gagatcgtcg agctgtgaca gattcgccat aaaagcggcg
    13381 acatcaccct cggcgttaaa ctctgcgggc agcacaggtt caatttcaat atccgccagc
    13441 tccagttcac gtcccgtttc acgagcgaga atcaatagct tacgcgccac atccatacca
    13501 gaaagatcat ctcgcgggtc cggttcggta taacccattt cccgcgccag tgtggtcgcc
    13561 tcggagaaac tcatgccttc gtctaacttg ccgaagatat aagaaagcga acctgaaaga
    13621 atgccggaga acttcaccaa ttcatcacct gcatttaaca gattttgcag gttctcaata
    13681 accggtaatc cagccccaac gttggtgtca tagaggaatt tacgccgcga tttttccgcc
    13741 gcatgacgca acagatggta gtaatccatc gacgaggtgt tagccttttt gttcggcgtg
    13801 acgacgtgga aaccttcgcg caggaagtca gcatactgat ccgccactgc ctggctggaa
    13861 gtgcagtcaa caatgaccgg gttcagcaga tgatattctt tcacgaggcg aattaagcgc
    13921 ccgagattaa acggctcttt ggcttgcgcc agttcttcct gccagttttc cagattaagg
    13981 ccatgtacat tggtgagcag agccttcgag ttggcaacac cgcagacacg taagtcgata
    14041 tgtttattct tcagccagct ttgctgacgc ttcagttgct ccagcagcgc accgccaacg
    14101 ccaccgacgc caatcacaaa cacttcgata acctgatcgg tattgaacag catctgatga
    14161 gtaacgcgca cgccagtggt cgcatcatcg ttatttacca cgacagagat tgagcgttca
    14221 gaagatccct gagcaatggc gacaatgttg atattggcgc gggccagtgc ggcaaagaat
    14281 ttcgccgaga tcccacgcaa ggtgcgcata ccatcaccta ccaccgagat aatggccagc
    14341 cgttccgtca ctgccagcgg ctccagtaag ccctctttca gttccaggta gaactcttcc
    14401 tgcattgccc gttcagctcg cacacagtcg ctttgtggaa cgcagaaact gatgctgtat
    14461 tcggaagatg attgcgtaat cagcaccacg gaaatacggg cgcgtgacat cgctgcaaag
    14521 acgcgcgccg ccatgccaac catccctttc atccccgggc cggaaacgct gaacattgcc
    14581 atgttattca gattggaaat gcccttgacc ggtaattcgt cttcatcacg gctggcacca
    14641 atgagcgtac ctggtgcttg aggatttccg gtatttttaa tcaggcaagg gatctggaac
    14701 tgggcgatgg gggtaatggt gcgggggtga agaactttag cgccgaaata ggaaagctcc
    14761 atcgcttcct ggtaggacat cgacttcaac aacctcgcat cgggcacctg acgcgggtcg
    14821 caggtataga ccccgtcaac gtccgtccaa atctcgcaac aatcggcgcg taaacaggca
    14881 gccagcaccg ccgcggagta gtcggaaccg ttgcgtccaa gcaccaccag ttcgcctttt
    14941 tcattaccgg cggtgaaacc tgccatcagc accatgtgat cagccggaat acgacttgcc
    15001 gcaatacggc gagtggactc agcaatatcg acggtagatt cgaggtaatg ccccactgcc
    15061 agcagttttt cgaccggatc gataacggta acgttgtgac cacgcgcttc taacacgccg
    15121 gccataatgg cgatcgacat tttctcgcca cggcaaatca gcgcagcgtt gatgctatcc
    15181 gggcactgcc ccaacaaact gatgccatgc aggacatgtt ttatttgggc aaattcctgg
    15241 tcgacgaaag ttttcaattg tgccagcgga aatcccggct gggcggcggc gagtcccgtc
    15301 agaagttcgg caaaaatacg ttcggcatcg ctgatattgg gtaaagcatc ctggccgcta
    15361 atggtttttt caatcatcgc taccagatgg ttggtgattt tggcgggggc agagaggacg
    15421 gtggccacct gcccctgcct ggcattgctt tccagaatat cggcaacccg cagaaaacgt
    15481 tctgcatttg ccactgatgt accgccgaac ttcaacactc gcatggttgt tacctcgtta
    15541 cctttggtcg aaaaaaaagc ccgcactgtc aggtgcgggc ttttttctgt gtttcctgta
    15601 cgcgtcagcc cgcaccgtta cctgtggtaa tggtgatggt ggtggtaatg gtggtgctaa
    15661 tgcgtttcat ggatgttgtg tactctgtaa tttttatctg tctgtgcgct atgcctatat
    15721 tggttaaagt atttagtgac ctaagtcaat aaaattttaa tttactcacg gcaggtaacc
    15781 agttcagaag ctgctatcag acactctttt tttaatccac acagagacat attgcccgtt
    15841 gcagtcagaa tgaaaagctg aaaatcactt actaaggcgt tttttatttg gtgatatttt
    15901 tttcaatatc atgcagcaaa cggtgcaaca ttgccgtgtc tcgttgctct aaaagcccca
    15961 ggcgttgttg taaccagtcg accagtttta tgtcatctgc cactgccaga gtcgtcagca
    16021 atgtcatggc tcgttcgcgt aaagcttgca gttgatgttg gtctgccgtt gtgtcacttt
    16081 tcgccggttg ttgtattaat gttgctaatt gatagcaata gaccatcact gcctgcccca
    16141 gattgagcga aggataatcc gccaccatcg gcacaccagt aagaacgtca gccaacgcta
    16201 actcttcgtt agtcagcccg gaatcttcgc gaccaaacac cagagcggca tggctcatcc
    16261 atgaagattt ttcctctaac agcggcacca gttctactgg cgtagcgtag taatgatatt
    16321 tcgcccgact gcgcgcagtg gtggcaacag tgaaatcgac atcgtgtaac gattcagcta
    16381 gtgtcttgaa aactttaata ttatcaataa tatcaccaga tccatgtgcg acccaacggg
    16441 cgactggctc caggtgtgcc tgactatcaa caatccgcag ttcgctaaaa cccatcgttt
    16501 tcattgcccg cgccgctgcc ccaatatttt ctgctctggc gggtgcaacc agaataatcg
    16561 ttatacgcat attgccactc ttcttgacca aataaccgcg aacagggtga tcattgtcaa
    16621 cttattacgc gttgcgaatt tacaaattct taacgtaagt cgcaggaaaa gccctttgct
    16681 tagcttaaaa aaagctaaac tatttcctga ctgtactaac gattgaattg ttaaaaaatg
    16741 ctacatatcc ttctgtttac ttaggataat tttataaaaa ataaatctcg ataattggat
    16801 tcaccacgtt tattagttgt atgaagcaac tagttggatt attaaaataa tgtgacgaaa
    16861 gctagcattt agatacgatc atttcatcaa actgttaacg tgctacaatt gaacttgata
    16921 tatgtcaacg aagcgtagtt ttattgggtg tcctgcccct cttagcctgt tatattgctg
    16981 ttaaaatggt taggatgaca gccgtttttg acactgtcgg gtcctgaggg aaagtaccca
    17041 cgaccaagct aatgatgttg ttgacgttga tggaaagtgc atcaagaacg caattacgta
    17101 ctttagtcat gttacgccga tcatgttaat ttgcagcatg catcaggcag gtcagggact
    17161 tttgtacttc ctgtttcgat ttagttggca atttaggtag caaacatgca gaccccgcac
    17221 attcttatcg ttgaagacga gttggtaaca cgcaacacgt tgaaaagtat tttcgaagcg
    17281 gaaggctatg atgttttcga agcgacagat ggcgcggaaa tgcatcagat cctctctgaa
    17341 tatgacatca acctggtgat catggatatc aatctgccgg gtaaaaacgg tcttctgtta
    17401 gcgcgtgaac tgcgcgagca ggcgaatgtt gcgttgatgt tcctgactgg ccgtgacaac
    17461 gaagtcgata aaattctcgg cctcgaaatc ggtgcagatg actacatcac caaaccgttc
    17521 aacccgcgtg aactgactat tcgtgcacgc aacctgctgt cccgtaccat gaatctgggt
    17581 actgtcagcg aagaacgtcg tagcgttgaa agctacaagt tcaatggttg ggaactggac
    17641 atcaacagcc gttcgttgat cggccctgat ggcgagcagt acaagctgcc gcgcagcgag
    17701 ttccgcgcca tgcttcactt ctgtgaaaac ccaggcaaaa ttcagtcccg tgctgaactg
    17761 ctgaagaaaa tgactggccg tgagctgaaa ccgcacgacc gtactgtaga cgtgacgatc
    17821 cgccgtattc gtaaacattt cgaatctacg ccggatacgc cggaaatcat cgccaccatt
    17881 cacggtgaag gttatcgctt ctgcggtgat ctggaagatt aatcggcttt accaccgtca
    17941 aaaaaaacgg cgctttttag cgccgttttt atttttctac cttatttcca gatacgtaat
    18001 tcatcgtccg ttgtaacttc tttactggct ttcattttcg gcagtgaaaa cgcataccag
    18061 tcgatattac gggtcacaaa catcatgccg gccagcgcca ccaccagtac actggttccc
    18121 aacaacagcg cgctatcggc agagttgagc agtccccaca tcacaccatc cagcaacaac
    18181 agcgcgaggg taaacaacat gctattacgc cagcctttca ataccgcctg caaataaata
    18241 ccgttcatta acgccccaat cagactggcg attatccatg ccacggtaaa accggtatgt
    18301 tcagaaagcg ccagcaagag caaataaaac atcaccaatg acagccccac cagcaaatat
    18361 tgcattgggt gtaaacgttg cgcggtgagc gtttcaaaaa caaagaatgc cataaaagtc
    18421 agcgcaatca gcagaatggc gtacttagtc gcccggtccg ttaattggta ttgatcggct
    18481 ggcgtcgtca ctgcgacgct aaacgcgggg aagttttccc agccggtatc attgcctgaa
    18541 gcaaaacgct caccgagatt attagcaaac caactacttt gccattgcgc ctgaaaacct
    18601 gactcgctaa cttcccgttt ggctggtaga aaatcaccta aaaaactggg atgcggccag
    18661 ttgctggtta aggtcatttc gctataacgc ccgccaggca ccacagaaag atcgccggta
    18721 ccgcttaaat tcagcgccac attcagcttc aggttctgct tgcgccagtc cccttcgggt
    18781 aaagggatat gcacgccctg cccgccttgc tctaacccgg tgccgggttc aatggtcagc
    18841 gccgtaccgt taacttcagg cgctttcacc acaccaatac cacgcgcatc cccgacgcta
    18901 atcacaataa atggcttgcc taaggtgata tttggcgcgt cgagttcgct aagacgcgaa
    18961 acatcgaaat cggcttttag cgttaaatca ctgtgccaga cctgaccggt atagatgcct
    19021 atcttgcgtt cttccacgtt ctgattgcca tcaaccatca atgactcagg taaccaaaaa
    19081 tgaataaaac ttcttttccg ctccacggtt ttatcgtctt cctgcaccgt ataaagctcg
    19141 gtcacaggaa tagcgatgag cggcccaacg agtttttgcg gcccgctggt actttggcga
    19201 atcacatctt ccacatcgct acggtaatcg gcacgttcga caatcacctg ccgaatcagc
    19261 attatcggaa tcaacaacag caatactgca ccaaacaagg tggtcatttt ccagaacagg
    19321 ggggatttca acatagtctt ctcctttgca atggcagcag gatacgaagg ctatgtggga
    19381 agaatttgaa gctatgtgaa gtgacggtga agtcgaagcg aggccagcac gccaccttcc
    19441 tgcacgttgc gcagcgtgac ttcgccgtta aacaaacggg cgacctcact gacaaacgcc
    19501 aaccccagac cgctgctttt ttgcccattt gcacgcggca aagagtaaaa gcgttcaaaa
    19561 atacgtgaca gcgcgtagtc aggaatccca ctaccggtat ccagcacctt aagggtgacg
    19621 tattcctgat ccacttcggc gcttagcgtt atgcaaccgc tctcgggagt aaaatcgatg
    19681 gcgttatcca gtaaattccc cagcgcctgc tccagtaacg ccggttcagc agcaacgtta
    19741 acctcagtag gcataacatg caaagtgatg tttttttctg ccaactgcac ggtgcgcgct
    19801 tcgctgacgc ggcgaaataa tgccgccaca tcaacagcag tcagaacgac ttcctgacga
    19861 ttctccagtc ttgcctggcg tagtaacgtt tccaccagtg cctgcattcg cgcattttgc
    19921 gtcagaatgt tgtcggtaaa acgagccacc acttccggcg gcggaccttc gcataaaatt
    19981 tccgccgcgc cacgaatcgc cgccagtggg ctttttagct catgagttaa cgcataaaca
    20041 tactgctcaa tatagttttt cccttccagc ttcacgcgca tactttccag cgcctgcgcg
    20101 agtttacgca actcgctact accgagatcg gggagaggaa cgggcttatt gtcagtgacg
    20161 gaatcagcat agcgagtgag ccgggcaata gagcggttga tccaccaaac catgcctgcg
    20221 ccaatcacca gtgcaatccc caacaaaatg gcgctggccc ataaaattcg ccgctcgcta
    20281 cgcttaatga ccggagccat cgccgcgttc ggtttgccta cgctcaaaac gccaataagc
    20341 cgcgagccgt ccataatcgg cgcggcaaca tacatcacag aactttcggg atcggcagga
    20401 ttttgcaacg tgctgcgcgc accatactga ccacgcaacg ttagccagac gtcattccag
    20461 cgcgaataat cctgtccaac ggctttattt gctgaatcaa acaatacttt gccctgcgca
    20521 tcggtcatat agacatgata ttcgttgcgc actttgttaa tgccaccgat attggcgcga
    20581 aacgggcgat gttgtagctg attaaacgcc tgcgccagtt gcccatgcgt tgggtcccca
    20641 gagagcaaat cgggacgcgc cagctccgcc agcaacgttg cggtgtcgat taacgtcccc
    20701 tcggttgctc ttcgcacgcc cggtttaact tctttgacaa aaatagccag aacgaaccag
    20761 gccgccaccg ccaccagtaa aaaatagccc agcaacaacc gcatgccgat acgcattaca
    20821 ggcccctcag gctatatccc atgccgcgat gagtattaat cggtgaaaga tcggggttga
    20881 tggcgcgcag cttggcacgc agcgttttaa tgtgggtatc gacggtgcga tcgtaggtgt
    20941 cctgcgcatc ttcccacacg ctatccatta gttgctggcg ggaccagacg cggcccggcg
    21001 acttgagtaa cgtcttcagc aataaaaact cataccgcgt cagcgttaat ggcgtgtcaa
    21061 accagctgat ctgcgccgca ggttcattca attcaaaatg accaatacgg ataacgggag
    21121 acggcgtcga gaacttcttc acccgacgca gtaaggtgcg cactctggcg cacacttcgc
    21181 gaggtgaaaa cggtttagcc acgtagtcgt cagcgccaat ttccagccca agcaggcgat
    21241 cgacctcttc atttcgggcc gtcaggaaca gtacaggtaa cgccggatgg agcgccagta
    21301 actggcggca caattcaaag ccgctgatat caggcaaacc aacatcgaga atcatgacat
    21361 cgggaacctg ctggcgagct ttatccagca ctggcaagcc tcgctcaaag acctcgacgt
    21421 caaaaccttc ctgttgcaac atgtagacca gcgtatcggc tatcccttgc tcgtcttcga
    21481 ctaaccagac cgtttcccgt tgcataaatc gcctctgtta ttgccgccac ggcatgacag
    21541 gaaccgcgct aatcgcgttt ttcggcgaac cttctacaac tttgtcggag taagccagat
    21601 aagcgagcgc gttgcgttta gcgtcataaa agcgcacgac ctgtaacgac ttaaagacca
    21661 gggacgtgcg ttttttgaat actacctcgc cctgagcttt gccgttttta atacgatccg
    21721 acagttcaat cggcccgact tgttgacaag aaatggccgc atcggaggta tcttctgcca
    21781 ggcccaatcc ccctttaata ccaccggttt tcgcccggct cacataacag gtgacatttt
    21841 tcacatcggg atcgtcaaag gcttcaacaa caattttgtg atccgggccg atcattttaa
    21901 atacggtgtc gaccgaacca atctcttctg catgagccaa tggccccagc attatcaggc
    21961 ttaaagacag gattaaatgc ttgtatttca tatcgttacc atttttatct attacacgag
    22021 caattagttc gtcacggtaa ataacatatt gattaagaac aaaatctcaa tacttttatt
    22081 tccgtcaaga cctaaaacat actcgactaa gaaaaaaaca ctgaatgcta aaacagcaaa
    22141 aaatgctatt atccaattac ctgatgtcag gtgctcgttg ttgaaaggat gaggatattt
    22201 tatggatcag gccggcatta ttcgcgacct tttaatctgg ctggaaggtc atctggatca
    22261 tcccctgtcg ctcgacaatg tagcggcgaa agcaggttat tccaagtggc acttacagag
    22321 aatgtttaaa gatgtcactg gccatgctat tggcgcgtat attcgtgctc gtcgtttgtc
    22381 gaaatcggcg gtcgcactac gcctgactgc gcgtccgatt ctggacatcg cgctgcaata
    22441 ccgcttcgac tctcaacaga catttacccg cgctttcaag aagcagtttg cccagactcc
    22501 tgcactttac cgccgttctc ctgaatggag cgcctttggt attcgcccgc cgctacgtct
    22561 gggtgaattc actatgccag agcacaaatt tgtcactctg gaagatacgc cgctgattgg
    22621 tgttacccag agctactcct gttcgctgga gcaaatctct gatttccgcc atgaaatgcg
    22681 ctaccagttc tggcacgatt ttctcggcaa ctcgccaacc attccaccag tgctttacgg
    22741 tttgaacgaa acacgtccga gccaggataa agacgacgaa caagaggtgt tctacaccac
    22801 cgcgttagct caggatcagg cagatggcta tgtactgact gggcatccgg tgatgttgca
    22861 gggcggcgaa tatgtgatgt ttacctatga aggtctggga accggcgtgc aggagtttat
    22921 cctgacggta tacggaacgt gcatgccaat gcttaacttg acgcgccgta aaggtcagga
    22981 tattgagcga tactacccgg cagaagatgc caaagcggga gatcgcccaa ttaatctgcg
    23041 ctgtgaactg ctgattccga tccgtcgtta acgctgcagc tcatctaatg caggggcgtc
    23101 cagatgcgag atgtcccctg cggtttcaac cacccagcca gacgccagcc acaagctttc
    23161 ctgataatcc acgcgcgaaa tagaacagtt acgcagacgt aagcggcgct ctgcccatgc
    23221 tggtaatccg agaatcgtac tcaccaggca tcccagtgca ataccatggc tcaccagtag
    23281 cggtcggctt ccctgcggta agtcccggca agattccagt gccgcattaa cgcgatcgct
    23341 gagctcctgc attgactcgc cttcaggaat acgcccgtca acggtgccat tgaccagttg
    23401 ccgacgccag ttctcttctt cttcggtgag tgaatcgata tttcttgttt ccagtacacc
    23461 catatttaat tcacgcaggc gagaatcaaa gatgatgtca cagccgcagg cctgggcgat
    23521 gatttccgcc gtgcgccggg tccgtcctaa atcgctactg atgatatgcg taatgccaag
    23581 ctctttggca cgggttgcca cctgcatcgc ctgttgctca cctttggcgg tcagcgggct
    23641 gtcagactgg ccctgaatac gtcgctcggc gttccactgc gtttcaccgt ggcggactag
    23701 gtatacctgt aacatgcttt ttttccgtat actgctgtaa cgttaatttt ctgagtaatg
    23761 ctgattatgc accaagttgt ctgtgcgacc accaatcccg ctaaaattca ggccattctg
    23821 caggcatttc acgagatctt cggcgaagga tcctgccata ttgcatccgt tgccgtcgag
    23881 agcggtgtac cggaacagcc ctttggcagt gaggaaacgc gcgctggcgc acgaaatagg
    23941 gtagccaatg cccgccgttt acttcctgag gctgattttt gggtggcgat tgaagctggc
    24001 atcgatggtg acagcacttt cagttgggtg gtgatagaaa acgccagcca gcgcggcgag
    24061 gcgcgttcgg ctactttgcc gttgcctgcg gtgattctgg aaaaagtgcg tgaaggtgag
    24121 gtgcttggtc ccgtgatgtc gcgttatacc ggtattgatg agattggtcg taaggaaggg
    24181 gcgatcggcg tgtttaccgc cgggaaactc actcgcacca gcgtctatca ccaggcggtg
    24241 atcctggcgc tgagtccgtt tcataatgcc gtgtatcagc cattgcaggc ctgattaggc
    24301 gttcacgccg catccggcat tcggtgcacg atgcctgatg cgccatgtcc tatcaggcct
    24361 acaaaatcaa tcgcttttca gcaacacgtc ttccagccac tggcgcagct cgacgggcgc
    24421 ggctttcaga ctattagatc cacgcgtaat cgtcgcgatg cccgcgccga gttcattttt
    24481 taactcacgc tggctcattt cgccgcgcaa cagctcttcg acaatacgca cgcgagtccc
    24541 caacgcttcg cgctcatctg gcgtcagcat caggtttaac aacggtaaat ggagatcgtt
    24601 ttggtaggca ttcttaagca ggtcgacaaa acgtaaccac tcctggtgac gctgttctgc
    24661 catcgctgct gaatagggtg attgttgggc catagtatgt cgccattgtt agcgggggaa
    24721 gcaaaatgcc tcccccggtt gtactcgcta aagagtacga tagcatatca taaacgtgtg
    24781 gatcagtaac gacgtcccca ttccgtggcg ctcatcaacg tcggtttatc ccccatgaaa
    24841 tagcggtagt aagcgtcata agccagcacg tttttcacat aaccgcgcgt ctcggagaat
    24901 ggaatactct cgacaaatgc cactgcgtcg atacgccctg cgctgttgcc aagccaggtt
    24961 cgcacccgcc ctggtccggc gttataagct gctgaggaga aaatacggtt attgccaaac
    25021 tgctgataaa catattgcag gtaactggtg ccaatgttga tgtttgtttc cggatccagc
    25081 aattgcccag ggctgctata accgggaata gagaacatct tcaccgtatg ggtcgcggta
    25141 ccaggcataa tctgcatcag gccgctggcc cctaccggtg atttcacttt cggattccag
    25201 gcactctcct gacgggcaat cgccatcgca tagctttgcg ggatctcctt accactggtg
    25261 tagcgcttga aaagatcgtt gtaagccagc gggaatcgct cttccagatg atcccacagc
    25321 ttcccggcga tcgttgcctg aacgctaaga tcccaccatt ggttattgaa agcataccga
    25381 gccagttgag cctgctctgt ttttgacttg ctcttcacca gattggccca ctcgctacgc
    25441 gcggtgttat cgagattcca gtacatcaac tcgcgcacgc gcgccatctc cggcccctga
    25501 gtcagggcgc tgtcaacatt ctgcggcgct ttatcaatct tcagctcata ctcttcgccg
    25561 atgcgttgtg cagcaaccat cgggtagaaa ccacgctgtt gcatgagttg atgcaaaatc
    25621 tcttttgctt cagcttcacg tccgcgttcc agcaataaat ccgcctgcca gtaacgccat
    25681 tcatctttct ctttcgcttc cataggcaga cgcgccagcc aggtattcag gccgcgtcga
    25741 tcgccggtgc caagcgccat tcgcacacgg cgttcaataa gcgaagtaga ttgcgagcgc
    25801 ataatcgcgt catcgcgcca tttcgcctgc tcgtcggtga catcgttgcc catcaaacgc
    25861 caggcgacaa tatcgcgcag ctcctgaatc tgatcttcat taagctgctg tgcctgagca
    25921 agcgacggga tcatcagccg tgcattttca gcatcctgcc gcgccacact ggcaaacgcc
    25981 accgccgcca tttgacgggt aaaatcggtc gcgccggttg tacgcgcgaa ggtcagtacc
    26041 gtattagggt tgttcgccag tgaaatgatt gccgaagcga tagtctggta atcggcaggc
    26101 atctgccctg ccagcactgt taccaggccg gtgttacccg ctttcatcgc cagacggata
    26161 cgctctaaat acgccagcgg atcttgttta cctgacgcac gccagacgct aaataacttg
    26221 tcacaggcgt taggctggct cttgccggtt agccacagct ctttcgcccc ttgccaggct
    26281 tcttcactct gcccggtgtt ccatttcgca tagtagtaat tacattgcgc ttcggtagtt
    26341 ccgggttttt ccgggctaaa ggctaacaag ccacgccagt cttcacgccg cgccagttca
    26401 ttgacgaaac gagattgcag cgtgcgagcg gggggaagcg tggggttagc gctaacaaag
    26461 ttagtgaccg tcaccgccgg ttgattcatc aggtcatcag tgatctggcg gtattccagg
    26521 tagggataaa gtgggtaatc tttcagtcca ggcatcattt gttcgaccac atccatttgt
    26581 cgattatccc aggcctgctt aatttgcgcg taacggctac gctgctcatc cagtgagtcg
    26641 gctcgcgcca cgctgctgac cgtcagcaga cagacaccga cagccaacag ccgccaggta
    26701 acgtgtttgg ctttttccac aagcgcatcc tctaagtgta aatacatcaa tgcagcgtca
    26761 tgccgcgtaa tagatttatg ctaaccagtc attgccgttt acgccacgtt acggacactt
    26821 ttttactttt acggggtgat cgactggctg ggattagaca gggaaacagg ctacacttcg
    26881 cctttgaaaa ctcatcatca cgataaacat agaggcgaag tccaacgtgg ctcaattcgt
    26941 ttataccatg catcgtgtcg gcaaagttgt tccgccgaaa cgtcatattt tgaaaaacat
    27001 ctctctgagt ttcttccctg gggcaaaaat tggtgtcctg ggtctgaacg gcgcgggtaa
    27061 gtccaccctg ctgcgcatta tggcgggcat tgataaagac atcgaaggtg aagcgcgtcc
    27121 gcagccagac atcaagattg gttatctgcc gcaggaaccg cagctgaacc cggaacacac
    27181 cgttcgtgag tccattgaag aagcggtttc tgaagtggtt aacgccctga aacgcctgga
    27241 tgaagtgtat gcgctgtatg ccgatccgga tgccgatttt gacaagctgg ccgctgaaca
    27301 aggccgtctg gaagagatca ttcaggctca cgacggtcac aacctgaacg ttcagctgga
    27361 gcgtgcggcg gatgcgctgc gtctgccgga ctgggacgcg aaaatcgcta acctttccgg
    27421 tggtgagcgt cgtcgcgtgg cgttgtgccg cctgctgctg gaaaaaccag acatgctgct
    27481 gctcgacgaa ccgaccaacc acctagatgc ggaatccgtg gcgtggctgg aacgcttcct
    27541 gcacgacttc gaaggcaccg tggtggcgat tacccacgac cgttacttcc tcgataacgt
    27601 tgcgggctgg atcctcgaac ttgaccgcgg tgaaggtatt ccgtgggaag gtaactactc
    27661 ctcctggctg gagcagaaag atcagcgtct ggcgcaggaa gcttcacaag aagcggcgcg
    27721 tcgtaagtcg atcgagaaag agctggagtg ggtacgtcag ggaactaaag gccgtcagtc
    27781 gaaaggtaaa gcacgtctgg cgcgctttga agagctgaac agcaccgaat atcagaaacg
    27841 taacgaaacc aacgaactgt ttattccacc tggaccgcgt ctgggcgata aagtgctgga
    27901 agtcagcaac cttcgtaaat cttacggcga tcgtctgctg attgatgatc tgagcttctc
    27961 gatccctaaa ggggcgatcg tcgggatcat cggtccgaac ggcgcgggta aatcaaccct
    28021 gttccgtatg atctctggtc aggaacagcc agacagcggc accatcactt taggtgaaac
    28081 ggtgaaactg gcatctgtcg atcagttccg tgactcaatg gataacagca aaaccgtttg
    28141 ggaagaagtt tccggcgggc tggatatcat gaagatcggt aacaccgaga tgccaagccg
    28201 cgcctacgtt ggccgcttta actttaaagg ggttgatcag ggtaaacgcg ttggcgaact
    28261 ctccggtggt gagcgtggtc gtctgcatct ggcgaagctg ctgcaggttg gcggcaacat
    28321 gctgctgctc gacgaaccga ccaacgacct ggatatcgaa accctgcgtg cgctggaaaa
    28381 cgccctgctg gagttcccgg gctgcgcgat ggttatctcg cacgaccgtt ggttcctcga
    28441 ccgtatcgcc acacacattc tggattacca ggatgaaggt aaagttgagt tctttgaagg
    28501 taactttacc gagtacgaag agtacaagaa acgcacgctg ggcgcagacg cgctggagcc
    28561 gaagcgtatc aagtacaagc gtattgcgaa gtaatgcgta aaatgccgga tgcggcgcgc
    28621 cagcatcgca tccggtaata gttaaatgat taatgggagg caagttcccg gaaaaaggca
    28681 ggattttctt taattgtggc aagtactttt gcagccaatc ccgtaggatt tcttcgcttc
    28741 gattcccagc ttttaatggt atccacactg gttcccagag cttttgccat ctcactttga
    28801 gaaacgttga gttgctcacg aatagcttta acatccgcaa tttcgtagcg ggtcacccgt
    28861 gcaggttctt ttaaaccttg tttaatctcg acagcctctt ccagagaggt tttcaactca
    28921 tcaaaaaaac tcattttata tctcgccttt gagcagtttc gtcaacttct ttagttgagc
    28981 tttttccgta tctgtcagac tatctttggc atttttcgga taggccataa tcaaataaat
    29041 aatctcttcc gtcgccagaa agtaaattat tctgacactg ccactttttc cctgtgaacc
    29101 cgcagccatt cggactttac gcaatccacc agtctgctga atgagatcac ctttatcggg
    29161 agttccgatg agttcttttt gtaattcctt taattcatca tcggttgcaa tctgcttgat
    29221 ctgacgcgta aacattggcg tctctataaa tattatcgcc ttacccattc cccctccctg
    29281 gcaggtacat tgtacaccta aatggagtac taagtacact tttaaatcag taatcccact
    29341 caacatctta taaagatgat tatctttgct cccccatcat ctcccgcacc agctccacgc
    29401 agcgcaggaa acggctgtcg taatcgtcct cttcaacccg cacgaattcg atattgttct
    29461 cttccagcat ctccaccagc aagttctgga actctttgcg atccaccgaa ctgccgaggc
    29521 tgcgtaaacc atccgccacc cacggcgtgt tgttctccag caggatcacc agatcgaaac
    29581 ggtattcatc aatcaacgcc tgcacgaacg gatgctcacg cccttcgtac tttttgcaga
    29641 acgcctgggt agtgacaaaa tcggtgtcga taaacgccac tttattggca tatttcactg
    29701 caaaatcaat atattgcgcg tgtcccagcg cgattttatc gtagtcggaa tactgcaacg
    29761 caatctcatc accgccaagg tgtgaaaaga cataatcgcg accatattcc catgcactgg
    29821 tagtgttgaa gatattggca agtttgttta ccagggtgga tttaccgctc gactcgccgc
    29881 caaggatcgc cacggtacgc acaaagaacg gcttaacttc cgtaggaata tattcccagt
    29941 agcggaacgg gttttcgcgg atctgcgcac cgctgatact cataaaggta cgcttcggat
    30001 cgaccagcac cgtctcgatc cccagatgtt ccatatactg cggcgcatcg gcttcttccg
    30061 aggtgtagat caggtccggc tgaatgcctt tttcggccat aaactttttg atgccgttgc
    30121 tccacacatc ccagccatgt ggatacggtt ccatgccctc ttcgttaaaa gcatgaatgc
    30181 ggatattttt ttgatactta aaggtctgca ataaccagcg cagacgatcc ggcacggttg
    30241 gctgctgcga catggcgctg tcttcgaaca atgcgcggtc gcgggtatcg tcaaaaccca
    30301 taatgatatg cagctcgtca acctggctac aggcgcgctg gataaggtag atatgtccag
    30361 tatgcagcgg gtaaaactta ccgaatacga caccgatcgt tttcttctgc cggggaaatt
    30421 caagtcccaa ataacggtgc aatgcctcca gcttttgtgc gctggggctt ttaattttgg
    30481 cattcagtaa ctggcttaaa taccctttgg tcataccgct ggcatcagct acctgctgta
    30541 gcgtgcagcc ctgttgcttg atggcagttt tcaggtaatc aaatgacgac atatctccct
    30601 ccgtatctct cattataagt cgtcgaacac gctaagcgcg tcggagagtt ttttaacgcc
    30661 aaaaatctgc atcccttccg gcgctttttt cggcacgtta gctgccggaa caattgcccg
    30721 gcgaaaaccg tgtttcgccg cttctgagat tcgttcctgg ccgctaggca ccgggcggat
    30781 ctcccctgcc agcccgactt caccaaacac caccagatcc tgcggtagcg gtctgtcacg
    30841 caggctggaa accatcgcca gcagtaacgc taaatcggca ctggtttccg tcaccttcac
    30901 gccaccgacc acgttaacaa acacatcctg atcggccatt tgtagaccac cgtgacggtg
    30961 aagcaccgcc agcaggattg ccagacggtt ttgctccagc ccgactgcca cgcggcgtgg
    31021 attcgccatc atcgagtgat cgacgagcgc ctgaatctcc accagcagcg gacgcgttcc
    31081 ttcccacact accatcactg agctaccgga ggtcacttca tcgccgcgac ttaagaaaat
    31141 tgccgaaggg ttgctgactt cacgcagccc ctgctcggtc attgcgaaga cacccagctc
    31201 attcaccgcg ccgaagcggt ttttatggct gcgtaaggtg cgaaaacgtg agtcggcatc
    31261 accatccaaa agcaccgaac agtcgataca gtgttccagc actttagggc cagccagcga
    31321 gccatctttg gttacgtgcc cgaccatgac aatcgccaca ccgcgcgttt tggcgaagcg
    31381 cgtcagataa gccgccgttt cacgcacctg tgccacgctg ccaggcgatg attgtacatc
    31441 cgccatatgc atcacctgga tggagtcaat taccatcagc ttcggttgct cttcttcggc
    31501 aatcaggcag atctgctcaa tgctggtttc cgacagcata ttaagattgt cagtcggcag
    31561 gccaaggcga tgagcgcgca ttgccacctg ttgcagcgac tcttcgccgg tgacatacag
    31621 cgttttcatc tgctgcgcca gtttgcacag cgtttgcagc aacagcgtgg atttccccgc
    31681 accagggtta ccgccaatca gaatggcgct tcccggcacc acgccgccgc ctaatacgcg
    31741 gtcgaactct ttaaatccgg tggaaaaacg cggcagctct tcaaggctga tgtcggaaag
    31801 tttctggact tttgccactc cggcgctacc ggcatagccg ctgagacgct cgttacgcgc
    31861 caccgttggc gacgctgcaa gacgcacctc ggtgatggtg ttccaggcat gacaggcact
    31921 gcactgcccc tgccagcgcg gataatcggc cccgcattca ttacaaacaa aggcgcgttt
    31981 tggagctttt gccacgatgt acctcgttaa tgctgtgccg cccgcaggat gacgggcgga
    32041 caattacttc tgattcaggc tgccagagag gatgcagaat acccccatca ggtcagcgtg
    32101 acggatggtg acttccgtct tttcattcac ttttggcttg gcatggtagg caatccccag
    32161 ccctgccgct ttgatcatcg gcaggtcatt ggctccatcg ccaatcgcca cggtctgcgc
    32221 cagcgggatt tcatactcct gcgcgaggcg ggtcagggtt ttcgctttat attgcgcgtc
    32281 aacgatgtcg ccgatcacat tgccggtaaa tttaccgtcc atgatctcca gttcattggc
    32341 taccacggct gtcaggcgca gcttgtcgcg caggtattca gcaaagaaag taaagccgcc
    32401 ggaggcaatc gccactttcc agcccagcgt ttccagcttg agtaccagct gcgttaagcc
    32461 aggcatcagc ggcagatttt cacgcacctg ttgcagaata ttggcgtcag cgcctttcag
    32521 cgtcgccacg cggctgcgca ggctggcggt aaaatcgagt tcgccgcgca tcgcccgttc
    32581 ggttacctcc gccaccctct cgcccgttcc ggccagtttg gcaatttcat caatacattc
    32641 aatctggatg gcggtggaat ccatgtccat caccagcaac cccggcgtgc gcaggtgtgg
    32701 gattttcccc agcggcgcga catccagttg cgcttcatgg gccaggcgcg tagcccgcgc
    32761 agtgagtgaa cctgccagac gaatcacctg ataatcttcc acgcaccagg cggcaacaat
    32821 caccatagcc gcgcccagtt tgctctggta ttgggtcaga cgttgtttat ccagcccacg
    32881 accatacagc agccagccgc tacgacctgc gtggtaatcc agtggcatca cttcatcacc
    32941 acttaatgaa agaggcagac caggccataa agagacatct tcaggcaggt cgcaccaggt
    33001 aatgttaggc attaaggctc ctgtaaaatc gttcgaagca gggaaaataa cgcatgaggc
    33061 taccttgtat ccattgcttc tggcaacatt aagtctcaaa ttttcaaagg gtggaagatg
    33121 gctcgcacaa aactgaaatt ccggctgcat cgggcagtga ttgtcctgtt ctgtcttgcc
    33181 ttgttagtgg cgctgatgca gggggcgtca tggtttagtc aaaaccacca gcgacagcgt
    33241 aatccgcaac tggaagaact ggcccgcacc ctggcgcggc aggtgacgct aaacgttgca
    33301 ccgctgatgc gtaccgactc accggatgaa aaacgcattc aggcgatcct cgatcagtta
    33361 acggatgaaa gccgcattct cgacgcaggc gtatatgatg agcaaggcga tcttatcgcc
    33421 cgttctggcg aaagcgtcga cgtgcgcgac cggctggcgc tcgacggcaa aaaagcgggc
    33481 ggctatttta accagcagat tgtcgaaccc attgcgggta aaaacggacc gctcggctat
    33541 ctgcgcctga cgctcgacac ccatacgctc gccaccgaag cccaacaggt ggataacacc
    33601 actaacattt tacgcctgat gttgctgctc tcgctggcaa tcggcgtagt gctgacccgc
    33661 acgctgctac agggtaaacg cacccgctgg cagcaatcgc ccttcctgtt aaccgccagt
    33721 aaaccggtgc cggaagagga agaaagcgag aaaaaagagt gacccattac tacaagaaag
    33781 gaaatcgtta tgtccacatt acgcctgctc atctctgact cttacgaccc gtggtttaac
    33841 ctggcggtag aggagtgtat ttttcgccag atgccggcta cgcagcgcgt tctgtttctc
    33901 tggcgcaatg ccgacacggt agtgattggt cgcgcgcaga acccgtggaa agagtgtaat
    33961 acccggcgga tggaagaaga taacgtccgc ctggcgcggc gcagtagcgg tggcggcgca
    34021 gtgttccacg atctcggcaa tacctgtttt acctttatgg ctggcaagcc ggagtacgat
    34081 aaaaccatct ccacgtcgat tgtgctcaat gcgctgaacg cgctcggcgt cagcgccgaa
    34141 gcttccgggc gtaacgatct ggtggtgaaa accgccgaag gcgatcgcaa agtctcaggc
    34201 tcggcctatc gcgaaaccaa agatcgtggc ttccaccacg gcactttgct gctcaattcc
    34261 gaccttagcc gcctggcaaa ctatctcaat ccggataaaa agaaactggc ggcgaaaggc
    34321 attacctcag tgcgttcccg cgtgaccaat ctgaccgaat tgctaccggg gatcacccat
    34381 gagcaggttt gcgaggccat aaccgaggcc tttttcgccc attatggcga gcgcgtggaa
    34441 gcggaaatca tctccccgga caaaacgcca gacttgccaa acttcgccga aacctttgcc
    34501 cgccagagta gctgggaatg gaacttcggt caggctccgg cattctcgca tctgctggat
    34561 gaacgcttta cctggggcgg cgtggaactg catttcgacg ttgaaaaagg ccatattacc
    34621 cgcgcccagg tgtttaccga cagtctcaac cccgcgccgc tggaagccct cgccgggcga
    34681 ctgcaaggct gtctgtaccg cgcggatatg ctgcaacagg agtgcgaagc gctgctggtt
    34741 gacttcccgg aacaggaaaa agagctacgg gagttgtcag cgtggatggc aggggcggta
    34801 aggtaactcg ctgattaaaa tatccaggga aggaaatatg atcgatctgg aaaaccagga
    34861 acgggagatt atcaatttaa tgttcagcca ggatatatcc tggctaaccg ccgtgcgtat
    34921 caggcataaa ctatcacttg ctgaagtaag taaaatgtta ggtatctcca ttaattctct
    34981 aaaacagatc gaaaagacgg aacggcttag ttcaaacatc aaaagcaaaa tggcaggaat
    35041 ttatggttgt ccaccagagt tgctgatatg cccttcctgg atgactgctg aacataaatg
    35101 aatagattcg tgaatagata cttgaatgga ttttattttc tatccatatg aaaaataata
    35161 gtaaatctat aaatcagttg ctattgatga ataaatacta aaaagccgga gcagtttccc
    35221 actccggctt cacacgacaa tcgcctttca gcaaaacaca attactcttt atcgcccagc
    35281 agaacggatt ccagcgcgat tttgatcatg tcgttgaagg tggtctgacg ctcagcggca
    35341 gtggtctgct cgtgagtgcg gatgtggtca gacacggtgc agatggtcag ggctttcgcg
    35401 ccaaattctg cagcgacgcc gtagatacca gccgcttcca tttccacgcc gaggatgccg
    35461 tatttttcca tcacgtcgaa catttcgccg tccggagagt agaacaggtc agcggagaac
    35521 aggttaccaa cacgagcatc aacgcccagt gcttttgccg cgtctaccgc gttacgcacc
    35581 atgtcgaagt cagcgatagc ggcaaagtca tggtctttaa aacggatgcg gttaactttg
    35641 gaatcggtgc aggcacccat accgataacg acgtcgcgca gttttacgtg cggcagaaca
    35701 gcgccacagg aaccaacgcg gataattttc ttcacgccga aatcagtgat cagttctttg
    35761 gtgtagatgg agcaggacgg gatacccata ccgtggccca ttacggaaat tttgcggcct
    35821 ttgtaagtac cagtgaagcc cagcataccg cgaacgttgt tcacttcacg ggcatcttca
    35881 aggaaagttt cagcaatata cttcgcacgc agcgggtcac ctggcatcaa aactacgtca
    35941 gcaaaatcgc ccatttctgc attaatgtgt ggagtagcca tcattttatc ctttttgtga
    36001 catattaaag gcggagtcac tgctccgccc aaatccatca gaacatggct ttgccatatt
    36061 ccatatcaga agtaccaaaa tattttgcca gagtctggcc gatatccgcg aaggtttcac
    36121 gatgacccag cgaacctggt ttcactttcg ggccgtaaac cagtaccgga atgtgttcac
    36181 gcgtgtggtc agtaccggtc caggtcgggt cacaaccgtg gtcagcagtg agaatcagaa
    36241 tatcgtcatc gcgcagcaaa gacatcagct ccggcagacg gcggtcgaac agttccagac
    36301 ccgcagcata accggcgacg tcgcgacggt ggccccagga agagtcgaag tcaacgaagt
    36361 tggtgaagac gatggtgtta tcacccgctt ctttcatctc tttgatggtg gcgtcaaaca
    36421 gcgcgtccag gccggtcgct ttcacttttt tggtgatacc gcagttggcg tagatgtctg
    36481 caattttacc gaccgaaacc acctggccgt gtttttcatc aaccagtttc tgcagcacgg
    36541 tcggtgctgg cggctcaaca gccaggtcgt gacggttacc agtacgctgg aagttaccag
    36601 ctttgtcgcc gataaacgga cgagcgataa cacgaccgat gttgtagccg ccgttggtca
    36661 gctcttcacg agcgatttcg cacagttcgt agagtttgtc cagaccgaaa gtttcttcat
    36721 ggcaggcaat ctggaacacg gagtcagcgg aggtatagaa aatcggcttg ccggttttca
    36781 tgtgctcttc gcccagctga tccagaatga ccgtaccgga agagtggcag ttaccgaggt
    36841 aacccggcag attagcgcgt tcaaccagtt tatccagcag ctcttgcggg aagctgtttt
    36901 cgtgatcgga gaagtatccc cactcaaaca gaaccgggac accggcgatt tcccagtgac
    36961 cagacggggt atctttaccg gatgacattt cgtgcgccca tgcgtacgcg ccgataactt
    37021 cagcgttgcc gtccattccc gccggaatga aaccggtaga accttcgtgt gctttcgcca
    37081 gccccagacg ggtcagattt ggcagattga gcgggccttt acgaccgtta tcagcttcgc
    37141 ctttggcaca agcttctgcg atatgaccca gggtgtcagc cccgacgtca ccaaagcgtt
    37201 ctgcatcttc tgtagcgccg atgccgaatg agtccagcac cataataaat gcacgtttca
    37261 tatgttctcc gtactcagtg cttcaaattt aaatgatcgg atcagtatac cgttattcgc
    37321 tgatacggcg atagacagtt ggtgtgcttt ccggtgcttt atcggcaagt ttaattgccg
    37381 ctttcaccgc tttcgccgct tcctgccagc tgttttcgtc tttcgcgtgg ataactgcca
    37441 gcggacgctg accgtctacc tggtcgccca gacgcgccat atcagtaaag ccgacgctgt
    37501 aatcgatggt gtcagatgcc tgacggcgtc cgccgcccat tgcaaccact gccatcccca
    37561 gcgcgcgggt atccatttca ctgacaaacc cttcggtatc agcatagact gctttcgtca
    37621 gcatcgctgt cggcagatac ttcgcgtagt tctcaacgaa gtcggtcggg cctttttgtg
    37681 ccgctaccat acgaccaaag acttctgccg ctttaccgtt gtccagcacc gcctgtaatt
    37741 tcgcgcgcgc ttcggcgtca tctttcgcca gtttgccgga gatcagcatc tccacgcaca
    37801 gtgccatcgt gacatcaaac agacgcgggt tacgatattc acccgtcagg aactgcaccg
    37861 cttcacgaac ttcaaccgcg ttacctgcac tggaggccag tacctgattc atatcggtga
    37921 gcagcgcggt ggtgcgcacg ccagcgccgt tagccacgcc aacaatcgct tcggcaaggg
    37981 cttcagagag ttcgtaggtc ggcataaacg cgccgctacc cactttcacg tccatcacca
    38041 gcgcgtccag accttccgcc agtttcttcg ccagaataga ggcggtgatc agcgggattg
    38101 aatccacggt tgcggtaata tcgcgggtcg cgtagaaacg tttatccgcc ggagccaatg
    38161 agctggtctg gccgataatc gccacgccga cgtctttaat aatttcgcgg aaacggttgt
    38221 catccgggaa aatgtcgaag ccagggatgg attccagttt gtcgagcgta ccgccagtat
    38281 gaccgaggcc gcgaccggag atcatcggaa tatagccgcc acaggcagcg accatcggcc
    38341 ccaacatcag cgaagtcaca tcgccgacac cgccggtcga gtgtttatca acaatcgggc
    38401 cattcagatg caggcttttc cagtcgagaa cggttcctga atctcgcatc gccatggtca
    38461 gcgagacacg ctcaggcatt gtcatatcgt ggaagaaaat ggtcatcgcg agggcggcaa
    38521 tctgcccttc ggagatagtg ttgtcgcgaa tgccgttgat aaagaaacga atttcttcat
    38581 cactcagcgc atgaccatca cgttttttac gaataatttc ttgtgcgaga aacaaggtaa
    38641 cctcccgtgg aaaagagacg aggtaatcgg ggaaccggat gagcatcgct gcatcatccg
    38701 gcaaatcaag ccttagtagc tgctggcgct cttaccgtcg ccgtggccca gagctttcag
    38761 caggcttgcc agcaagctgg aagcaccaaa gcggtagtga cgcgcatcag cccagtcagc
    38821 accgaacagt tcatctgcaa tggcgagata tttctgcgca tcttccgcag tacgcacgcc
    38881 gcccgccggt ttgaaaccaa cggttttttc tacgcccata tcgcggatca cttccatcat
    38941 gatgcgcgcg ctttccggcg tcgcattcac agccacttta ccggtagagg ttttgatgaa
    39001 gtcagcaccc gctttgatgg agatttcaga cgctttacgg atcagcgctt cgtctttcag
    39061 ttcgccggtt tcaataatca ctttcagcag tacgttcgcc gcagcgcaag cctttttaca
    39121 ggctttcacc aggtcaaaac caacctgctc gttacccgcc atcagcgcgc ggtacgggaa
    39181 caccacgtca acttcatcgg caccgtaggc gattgccgca cgggtttctg ccagcgcaat
    39241 gtcgatgtcg tcgttaccgt gtgggaagtt agttaccgta gcgatacgga tttccggggt
    39301 gccctgctct ttcagagttt tgcgagcaat cgggataaag cgaggataga tacagatagc
    39361 ggcggtattg ccgaccggag ttttggcctg atgacacagg gcgatcactt tctcatcggt
    39421 gtcgtcgtca ttcagggtgg tcaggtccat caatttcagt gcacgcaggc tgcttgcttt
    39481 cagatcagtc atttcattct cctggcttgt cgccaataaa attcaccttg cgagtttgtt
    39541 agtattctaa catctactcc gcaacacact tcgatgtaca tcacaattaa ggaaatctac
    39601 ttacaagttt gcatcactgt aatgcgatct ggttcaaata attcactttc aaatgaatgc
    39661 gtcagtggtg gcaaacgcat caggatcaaa gtgaacatca cgaaacttct tacaatggcg
    39721 catcaccttc ggcataaaag gaaaagaaga tgcccacttc tcatgaaaat gcactgcaac
    39781 aacgttgcca gcaaattgtc accagcccgg tacttagccc ggagcagaag cgccattttc
    39841 tggcactgga agcagaaaac aatctgcctt accccgcgtt gcctgccgaa gcccgccgcg
    39901 cgctggatga gggggtaatc tgcgatatgt ttgaaggtca tgcgccgtac aaaccgcgct
    39961 atgtcttacc cgattacgcc cgttttctgg cgcacggttc cgaatggctg gagctggaag
    40021 gcgcgaaaga ccttgatgac gcactctctc tgctgaccat tctttaccac catgtaccgt
    40081 cggtcacatc gatgccggtc tacctggggc aactggatgc gttgttgcaa ccgtatgtta
    40141 gaattctaac acaagacgag atcgatattc gaataaaacg tttctggcgt tacctcgaca
    40201 gaaccctgcc agacgccttt atgcatgcta atatcggccc gtctgattcg cccatcaccc
    40261 gtgcgatatt acgtgcagac gcagagctga agcaggtttc accgaacctg acctttatct
    40321 acgatcctga tatcacccct gatgacctgc tgctggaagt ggcgaaaaac atctgtgaat
    40381 gtagcaaacc gcacatcgcc aacggtccgg tgcatgataa aattttcaca aaagggggct
    40441 acgggattgt gagctgttac aactcactgc cgctggcggg tggtggcagc acgctggtac
    40501 gcctaaacct gaaagccatt gccgagcgca gtgaatcgct ggaggacttc tttacgcgca
    40561 ctctaccgca ctactgccaa cagcagatcg ccatcatcga tgcgcggtgt gaattcctct
    40621 atcaacaatc acacttcttt gagaatagct tcctggtgaa agaagggctg attaaccctg
    40681 aacgttttgt gccgatgttt ggcatgtatg ggctggcgga agcggttaac ttgctgtgtg
    40741 aaaaagaagg gattgccgca cgctacggta aagaggccgc cgcaaatgaa gtgggttatc
    40801 gcattagcgc gcaactggcg gagtttgtcg ccaatacccc cgtgaaatat ggctggcaaa
    40861 aacgcgccat gttacacgca cagtcgggga taagttccga tatcggcacc acgccgggcg
    40921 cgcgtttacc atatggcgat gagccagatc cgattaccca tctgcaaact gtcgccccgc
    40981 atcatgctta ttattattcc ggcatcagcg acattctgac gctcgacgaa accatcaaac
    41041 gtaatccgca ggcgctggta cagctttgcc tcggtgcctt taaagccgga atgcgtgaat
    41101 ttaccgccaa tgtcagcggt aacgatctgg ttcgcgttac cggttatatg gtgcgtttgt
    41161 cggatttaga aaaatatcgc gccgaaggtt cacgcaccaa cactacctgg ctgggagaag
    41221 aagccgcacg caacactcgt attctggaac gccagccgcg cgtgataagc catgaacagc
    41281 agatgcgctt tagtcagtaa gattatcccc ttctcctgcg ttgacgggcc aggcagtcgt
    41341 ctggctctgt ttttgcaggg ctgcaatctg cgctgcaaaa actgtcacaa tccgtggacg
    41401 atggggcgct gtaatgattg cggagagtgc gtgccgcagt gtccgcatca ggcgttgcag
    41461 attgtcgacg gcaaagtggc gtggagcgcg gctgcctgcg agcaatgtga tacctgcctg
    41521 aagatgtgtc cgcaacatgc tacgccaatg gtgcaatcca tgagcgtgga cgaggtgctt
    41581 aaccatgtcc gcaaagcggt tctgtttatt gaagggataa cggtaagcgg cggcgaagcc
    41641 acgacccagt tgccgtttgt ggtggcgctg tttactgcca tcaaaaacga tccgcaactg
    41701 tgccatctca cctgcctcgt ggacagtaac ggcatgttga gcgaaaccgg ctgggaaaaa
    41761 ttgctcccgg tgtgtgacgg cgcaatgctc gatctcaaag cgtgggggag cgaatgtcat
    41821 cagcaattaa ccggacgcga taatcagcag atcaagcgca gcatctgttt gctggcagag
    41881 cgcggtaagc tggcggaact gcgtttgctg gtaatccctg gccaggtgga ttatttgcaa
    41941 catatcgaag aactggcggc gttaatcaag ggacttggcg atgttccggt acgcctgaac
    42001 gcgtttcatg cccacggcgt gtatggcgag gcgcaaagct ggccgagcgc cacgccggag
    42061 gatgtggaac aactggctga tgcgttacgg gagcgcgggg tgagccggtt aatatttccg
    42121 gcgctatatt tgtgacatgc cggatgtggc ctacggatcg gcacagacct gtaggcatga
    42181 taagacgcgt caagcgtcgc atcaggcatt gtgcgccagc tgccggatgc ggcgtgaacg
    42241 ccttatccgg cctacggttc ggcacagact tgtaggcatg ataagacgcg tcagcgtcgc
    42301 atcaggcatt gtgcgccaac ggccggatgc ggcgtaaacg ccttgtccgg cctacggcac
    42361 gttaaacaac gtataggtgt tattaagcaa cacttcggca atctcatccg ccggttccgg
    42421 tcgtaactcg caaagcacgg caaacacacg ggcggcctgt tcagggcgat taggctgccc
    42481 ctgaaaacca ttgagcggca tatctggtgc gtcggtttcc agcaacaacg atgccagcgg
    42541 taattttgcg atgacatcgc gggttttact ggcgcgtgga taggtgatag tcccgcctac
    42601 gccaattttg tagcccagtt gcacaaaccg ttcggcctgt tgcaggctgc cggaaaaacc
    42661 atgaaccacg ccagtgcacg gtaaatcgtg gcgtttaaga tgcatcgcca gtttgtcgtg
    42721 cgtgcgtcgt gaatgcagga tcaccggcag atcgtagcgt ttcgccagtt tcagttgttc
    42781 gtcgagtaac cactgctgcc tctcaaattg cggatcgtcg ccaaagagat ccagaccgat
    42841 ttcccccacc gccaccacct tcgccggatg cctttccagc gcctgctgta gctgatcaag
    42901 agacacatcg ctatgttttt ccaacatacc aggatgcaag cccaatgcgg catacagcgg
    42961 ttgatatttt tccgctaacg cctgcacgcg ggcaaaattt tccgcctctg tcgccggaac
    43021 aatgatcttg cctacgcccg cttgtgccgc gcgttgcagg ctggcctctt catcgccact
    43081 aaacggcggg aaatcaaaat ggcagtgggt gtcgataaaa cggcaaatca agccagatcc
    43141 tcattgttaa atgtcgggtc gttcgcctgc ggagcgtcgc tcacttcggc aaccagcgta
    43201 tcgttggcga ctggcgcagg cggaataacg atcgactccg gcgtcaccac cggcaccaga
    43261 tggcggctca gcggcgcttt ttcagtcaat aatttaccga ccgttgccag aaaataacgc
    43321 ccacataaac gcccaagttt atagtcttca cgcagcgccg gaatccgact gccgagcgcg
    43381 atactatgca atggcttcgg cggataaatt tcgaatatcc gcagctggcc cggtggtttc
    43441 tcaataaatt gctgaatctc acgatagctg gtttcatgat gctgcaccag attgaccagc
    43501 ggctgcaggc tactgtcacc cagccagcgt tccatgcgtt tgaaccactg tggcgtgtag
    43561 tacatttgtg acggcacagt gcggatgacg accaacgttt ttgctccctg ccttgccgcc
    43621 tctttaaccg ggatcgcatc actgatccca ccatccaggt agttaatacc ttccagcgac
    43681 actccgctac gataaaagcc aggtatcgca ctggaggcgc gaatcacatc cagccagttt
    43741 tgtttagttg gcaaaaagta attcggcgca tagtcatcct gacgacaggc gcacatataa
    43801 aacgatttgc cgctgtcaaa taaccgcgct gcggtgtcca tttgcaacgg catctggctt
    43861 gcggtggcct ccaccagcca gtcgagatcg ataagatttc ctccacggac aaagcgcaat
    43921 ggatcgaaaa attcgcgttt tgtggtgtaa cgcatgatga ctttgcgcgc gtaaccgggc
    43981 tgattgcaga tatacgccga gaggttctgc gccccggcag atgtgccgag ataaagatcg
    44041 aaaggattaa actgcgcgcg cataaactca tccagcacgc cagccgtgaa aattccacgt
    44101 tgtccgccgc cttcgcacac cagagcaatc cgcccaggct ggaacggcct tagcgacaac
    44161 ggcgcaatat taccaagcgt tacaggtatt cgctgcccca cctctgcctt cctgtttttg
    44221 ttgtgaaaga atgacaaagt aacgcaattt attattcgct aaaaccgcaa aagccagtcc
    44281 gctggactgg ctttggataa aattttctta atggtctgaa atctagggtc gttttcggcc
    44341 catgaacaaa ctcaccagga acagaataat cccgacgaca aagacaattt tagctgcgcc
    44401 tgcagcagta ccggccagac caccaaaccc aagtgcggcg gcgattaacg cgataaccag
    44461 aaatatgatg ccccaacgaa acataagcgt ctcctttacc atagttaatg tcaccgcttg
    44521 atatgagcgt gaaaatcact caatgcgata tttttagtgt ggtgcacatc gcgcctcccg
    44581 acaaagttcg ggaggtcgaa ttacgacgaa ttacttagtt ttcagatcat ttttaacgct
    44641 tttcacacca tctaccgctt tggcgatact ttcagcacgg tcgctctgtg cctgagaatc
    44701 tacggtaccg gagagctgga ccacgccatc ggtggtttca actttcacat gacgggaagg
    44761 gacaatgtca tctgccagca gtttggcttt gatttcactg gtagtggcgg tgtcacccgc
    44821 gtagcctttc acagagcctt ctttagcgtc gcgaacgtgc agtttgtcgc tgacagaggt
    44881 cacgccctca acacctttcg ccactttcac cgcctcttcg gcctgagcct ggctttcaac
    44941 gaaaccgctc agggtcacga ctttttgatc ggttttgaca gagatatcgg tgctcttgat
    45001 gttgtcatga tccaccaggg ccgccttcac tttcgcggtg atggcgctgt catccatgaa
    45061 attaccgact ttattcatag agctatcgac tttttgccct gcgctttcat tggtagtctg
    45121 cgcgttgttt tctgcgtagg cagagccggt cgcgacggca gaggtcaaca ttacagccag
    45181 cagagttttc gaaatcttca gtcttgtcat agtcatcgat ttattcctgt atgtttgctc
    45241 gtaatttgag cctggcaaca cgaggttgca ttgctgaata gggagagact tcaccctcta
    45301 cagaagtcaa tggtcgccat cacaaaagcg atgggtgatg aataacgacc attacagcct
    45361 ctgaatcagt tattaatatc ggtagaatga caatagacgg ctttagatac tgatatctac
    45421 gcattgaacg gtatttaacg ccgtcagaaa tgtcattact ttgttaaata tagatcacaa
    45481 ttttgaaacc gctcgggata tcagctaaaa cataagcaaa agtgaatgtt ttaagaacat
    45541 tccgtaagcg gctaataagg aagggaaatt gacagggcgc agcggctacc gcgccctgga
    45601 ggcaagaatt aatgctcgcg ggtctggtgg aactgaacgt ccggataacg ttcctgcgcc
    45661 aggcgcaggt tgaccatgct ggtagcgatg taagcgaggt tatcgccgcc atcaagcgcc
    45721 agttggcttt cgttcttacg cttgaactct tcgaatttct tcgcgtccgc acattctacc
    45781 cagcgggcag tggcaacgtt gactgactca tacactgctt caacgttgta ttcgctcttc
    45841 aggcgcgcta ccaccacatc aaactgcagc acaccaaccg cgccaacgat caaatcgttg
    45901 ttggagatcg gacggaacac ctgcaccgcg ccctcttcgg aaagctgtac cagccctttg
    45961 agcagctgtt tttgcttcag cggatctttc aggcggatac gacggaacag ttctggcgcg
    46021 aagttcggaa taccggtgaa cttcatcatc tcaccctggg taaaggtgtc gccgatctga
    46081 atggtgccgt ggttgtgcag accaaggata tcgccaggat acgcttcttc aacgtgcgaa
    46141 cggtcacccg ccataaaggt cagcgcgtcg gagatcacca catctttcgc ggtgcgcacc
    46201 tggcgcagct tcatgccttt ttcatattta ccggatacca cgcgcataaa cgccacgcgg
    46261 tcgcggtgtt tcgggtccat gttggcctga attttaaata cgaagccggt aaatttatct
    46321 tcgctcgctt ctacggtacg ggtatcggtc tgacgcggca tcggcgcagg cgcccactcc
    46381 accaggccat ccagcatatg atcgacgccg aagttaccca gcgcagtacc aaagaatacc
    46441 ggggtaattt cgcccgcaag gaacagctct ttgtcgaact cattagacgc gcctttcacc
    46501 agctccagtt cgtcacgcag ctgctgtgcc agatcttcac caaccgcagc gtcgagatcc
    46561 gggttattca gccctttaac aatgcggact tcctgaatgg tgtggccttt accgctctga
    46621 tagagatagg tttcatcttt ataaaggtgg taaacgcctt taaacagctt gccgcagcca
    46681 atcggccagg tgatcggtgc gcagccgatt ttcagctcgt tctcaacttc atcgagcagt
    46741 tccatcgggt cgcggatatc acggtcaagt ttgttcataa aggtgaggat cggcgtatcg
    46801 cgcagacggg taacttccat cagcttacgg gtacgatctt ctacaccttt tgcggcgtcg
    46861 ataaccatca ggcagcagtc caccgccgtc agggtacgat aggtatcttc cgagaagtct
    46921 tcgtgccccg gggtatcgag caagttaacc aggcagtcgt gatacggaaa ctgcatcaca
    46981 gacgtagtga tggagatccc acgctgcttt tccatctcca tccagtccga cttagcgtgc
    47041 tggttagagc cacggccttt tactgtaccg gcggtctgaa tggcctgtcc gaacagcagc
    47101 accttctcgg tgatggtagt cttaccggcg tccgggtgag aaataattgc aaaggttctt
    47161 cttttcgcta cctctgcggc caaaaggctt gtcataattg ttgtctcttt gattatgtac
    47221 aatcatatgt acaaagaaaa attcatgccg ctgattgtac attttggagg tatcagagca
    47281 tagcagaaca tgatcagtag gtgtatttgt agccaaagaa catttgtaca tgcagcaatc
    47341 tttgctctat tgacactgtt tatttataca gttaaagata atgctgtatg caaacacagt
    47401 gtagagggag ttttatgcgt attgagatct gcatagccaa agagaaaatg actaaaatgc
    47461 caaacggtgc tgtggatgcg ttaaaggaag aattaacccg acgcatcagt aagctttatg
    47521 acgatgtgga tgtgatcgta aaatctgcca gcaatgatgg actgacaatt ttaagatccg
    47581 tggataaaga gtctgctcaa aagttcgttc aggaaacgct gcaggaagtc tgggaaaccg
    47641 cggacgactg gtttattcgg taaatcttct ttacggggag ttaacttact ccccattttt
    47701 tattgcatga taattgcagc aacggcacag agtgtcacca caccacccag ccacacaaac
    47761 agcatgaagc acattgctcc cgctgtataa cgttttctcc cgtacatacg gcatttgtct
    47821 ttattcatca tctgcccctg aatcacctga agacgctgca ggtccggtaa cactgaatca
    47881 ctccgcacac gcaagatgtt tcacgctcac accatacagc ccgtttttcg ccattcccgt
    47941 atacagaaag gtattcaccg tcttcgtgtg aatacccatt tcactggcaa ggcgacaggg
    48001 tctcgactgg cggtagacgt agccaaacat caccaccatc tctgacatcg tcagcggacg
    48061 ggcaacgctg ttatacgtcc atacaccatt gcgtatggca tcacttatct gctcaccatc
    48121 acggagtgat tccgcaccca gttgcagaaa atgccgtccc tggaacatgc agcgcatcag
    48181 tctgcccagc aacgtatcac agattatcag ccaggattcg tcaaagtccg ggatataacg
    48241 gcggttctcc ggcaggaaaa actccctcgc cagtatcaca ctgtgttcct tcagtaacgc
    48301 ctgcagacca tactccagat agcgattcct ggtagccaga atcaccttca tcgcagcacc
    48361 ttcctgtttt ttatgcctca tgaaaaaaac ggcaggcaaa actcctgtgc ctgccgtcat
    48421 gcttcatgtc actgtcagca attaaaactc gaagttcaca cctacgttgt aggaaacatc
    48481 accgtcgctg gctgcaatac ctgctttggc tgc
//
LOCUS       DAZFFE010000022        48300 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFFE010000022 DAZFFE010000000
VERSION     DAZFFE010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.022"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(120..1784)
                     /gene="glnS"
                     /locus_tag="ACHJ69_001497"
     CDS             complement(120..1784)
                     /gene="glnS"
                     /locus_tag="ACHJ69_001497"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO5014513.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2004..2765)
                     /locus_tag="ACHJ69_001498"
     CDS             complement(2004..2765)
                     /locus_tag="ACHJ69_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5014514.1"
                     /translation="MKGYNRLEQIMDYLKSHNLVTVDELVAVTNASPATIRRDLIKLD
                     EQGVISRTHGGVTLNRFIPTQPTTHEKMQRSLAEKHAIASAAASMVKAGDSVVLDAGT
                     TMIELARQITHLPLRVITSDLHIALFLAEFKQIEVTIIGGRIDDSSQSCIGEHGRKLL
                     QNTWPDVAFLSCNSWDLEKGITAPTEEKAALKRDLIAHASRKILLADSSKYGSWSLFN
                     IAHLNELTEIITDSQLDGQTRRTLASLSTRLIIAD"
     gene            complement(2767..3753)
                     /locus_tag="ACHJ69_001499"
     CDS             complement(2767..3753)
                     /locus_tag="ACHJ69_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-threonate 4-phosphate dehydrogenase"
                     /protein_id="HFO5014515.1"
                     /translation="MNNIIAVTMGDPAGIGPEIIIKSLSEAGLAGAPVVVVGCVRTLR
                     RIMAMNITPQAELRVIQKVCDAHFAPGVINVMDEPLANPDALTPGVVQAAAGDLAYRC
                     IKRATALALSGEVKAIATAPLNKEALHLGGHNYPGHTELLAHLTGSKEYAMVLYTDKL
                     KVIHISTHISLRKFLDTLNGERVKTVIRVANHFLKRVGIERPRIAVAGVNPHAGEHGL
                     FGTEEIEIIAPAIEAMQAEDIDVTGPCPPDTVFMQCHEGLFDMVVAMYHDQGHIPLKL
                     LGFYDGVNITAGLPFIRTSADHGTAFDIAWTGKAKSESMAVSIQLAMQISRE"
     gene            complement(3746..4981)
                     /locus_tag="ACHJ69_001500"
     CDS             complement(3746..4981)
                     /locus_tag="ACHJ69_001500"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HFO5014516.1"
                     /translation="MEKGITKPSILVVADDFTGANDAGVSLAQVGHTVDVAFEMPYRG
                     DASVWVINSDSRAMDPKLAAMKITSLMSHLPLANNPPLVIKKIDSTLRGNIGAEIEAL
                     MKACGITGAVVAPAFPQAGRTTVAGECWVNGVRITETEFASDPKTPVLSARIADIIRL
                     QTAIPCQPVTVSQLSHLSYEQPWISVIDAQTDSDLDRIAAAVMQAKQPLLLVGSAGIC
                     DAVARRSAIMPPPTVLAIIGSMSEIAQRQIATLHSHPCITQIYVDVEHILAGNASDYD
                     ARIVQALQKGDHCIVHTCNDSVARHQIDTLCQRWQMSRAALGEKICRFLGELTRQVLL
                     RTMPDALYLSGGDVAMATASALGATGFRITGKVAQCVPYGHFLGGVWSRSVMTKAGGF
                     GDETTLHQVLNFIEEKCSE"
     gene            5151..6308
                     /locus_tag="ACHJ69_001501"
     CDS             5151..6308
                     /locus_tag="ACHJ69_001501"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFO5014517.1"
                     /translation="MVIINSTILSGAGAIPSLTSLLPDIRKMLLVTDRNIAQLDGVQQ
                     IRALLEKHCPQVNVIDNVPAEPTHHDVRQLMDVLGDASFDVVVGIGGGSVLDVAKLLS
                     VLCHPQSPGLDALLAGEKPTQRVQSWLIPTTAGTGSEATPNAILAIPEQSTKVGIISQ
                     VLLPDYVALFPELTTSMPAHIAASTGIDALCHLLECFTATVANPVSDNAALTGLSKLF
                     RHIQPAVNDPQDLRAKLEMLWASYYGGVAITHAGTHLVHALSYPLGGKYHLPHGVANA
                     ILLAPCMAFVRPWAVEKFARVWDCIPDAETALSTEEKSHALVTWLQALVHQLKLPNNL
                     ADLGVPPEDIASLSEVALNVKRLMNNVPCQLDLQDVQAIYQTLFPQHQFKE"
     gene            6312..7199
                     /locus_tag="ACHJ69_001502"
     CDS             6312..7199
                     /locus_tag="ACHJ69_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HFO5014518.1"
                     /translation="MPKTIEGVLTAIVTPFTATGSLNIPALKVQVNRQLEAGNAIFCG
                     GTNGEFFVLNEQEKLSVTQTCVDEVAGRAPVVAHIGEISTRETIRLGQQIEKLGVDAV
                     SVIAPYFVPLKQEELIAHYSAIADALSIPMFLYNIPARTGNTIQPETARALASHPNII
                     GIKDSAGSDESLKGFLDAVRDIDGFNVLNGPDSLIHKGFVEGCSTCISGLANIAPREI
                     NAIWSRFHAGDIEGSYEAQESVTGLRTDLYKVAFSPAAVKKALQLMGHDVGDSRYAVN
                     FTPEQISEIQQIIRHYNIN"
     gene            7259..8017
                     /locus_tag="ACHJ69_001503"
     CDS             7259..8017
                     /locus_tag="ACHJ69_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO5014519.1"
                     /translation="MFLWFMQPDIWLTLITLTFLEIILGVDNIIFLSLVVAKLPLSQQ
                     NMARKLGLSCAMIMRILLLISIAWLSHITQPLFTVLTLEISFRTLILLGGGLFLIYKA
                     IQEIREELSPAQEDEHGSGSRQLSLCGAIVQIMLLDIVFSLDSVITAVGLSQHIFIMI
                     AAVMIAVGVMMFAAKTIGDFVNATPSIKILALTFLLFVGVLLVADSLNIHIAKEYLYF
                     AIFFSLSVETLNIIRERKNKKVSTLIETHESHSH"
     gene            8053..9222
                     /locus_tag="ACHJ69_001504"
     CDS             8053..9222
                     /locus_tag="ACHJ69_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HFO5014520.1"
                     /translation="MSVALIRDGVIRPSENDSHVLTAMLPSSCPQNHAANILPLPDGA
                     LMCVWFAGTQEGIADISVWGSRLPAGGMQWSDAVKLSHDDTRSEQNPVLFLAPDNVLW
                     LLWTAQISGNQDTAIVRYRKSDDLGQTWGEIATLLDKPGTFIRQPITVLDNGNWLLPV
                     FYCRTQPGEKWVGNDDISAVKISADGGHSWRDVEVPQSLGCVHMNITMLHNGTLVAFF
                     RSRWADNIYISHSADNGESWSVPQATELPNNNSSIQVTTLASGELALVYNAMSAAGAV
                     EHRASLYDEIDDGDDSRKEPTAVGRSAFWGAPRAPMTVAISADGGKSWPWRRNLDEGD
                     GYCMTNNSLEKLNREFSYPSIKQSPDGTLHIAYTWWRQAIKYVRISPEWVKGQAS"
     gene            9219..9689
                     /locus_tag="ACHJ69_001505"
     CDS             9219..9689
                     /locus_tag="ACHJ69_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO5014521.1"
                     /translation="MIIGNLNHLSLAGLPAWVRNILLRPECSLSALSTREDGRWQPEG
                     CRWFCTLGTSDTQPAELRHTEYHHLWADIQVVITGCEGINAGTRPIARENDEERKPDL
                     FIAPSPENSVAITLHAGDFAVFMPEEPHQALCAIGTATSIRKAVFKVPRDMLEA"
     gene            9703..10983
                     /locus_tag="ACHJ69_001506"
     CDS             9703..10983
                     /locus_tag="ACHJ69_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO5014522.1"
                     /translation="MTMEMILALGILVLMIVLIMSDKMPFGAPPLLACLLLVVSGLST
                     VQQAFAGFVNPSVVMIAGFMVVMAALQKTRLISNVKSAMISLVNKGSYRSYGLLLVIV
                     MLGASLAGTGATGYYVLILSLVSTIPYSKKLPTSKLMMPLGFATNHPLLPINLALLFG
                     VTATVLETAGFHQEISMGRFALVNLIMSAAFLAWSLIAYRFLPDHPIADASEDALAAR
                     EETFNALPAWKEYCTIAAFAVSVIGMMLMNILGNIAFVIPGLAGAFVLMIDVLDFKEV
                     RDHMGAPVILMMAGVIGVADALAGTGFTAMVGDAVAGVLGSGVSPFVFIVAFALLTST
                     CATFTGSNMGSVYIFAPIAIAACTSLGLNPTAAAIAVVISGWNGGYMPIDGIPAMILG
                     MGKYKLPEFWVFSVPMYLIRILALCAGAVFIFPM"
     gene            complement(11072..13018)
                     /gene="nagE"
                     /locus_tag="ACHJ69_001507"
     CDS             complement(11072..13018)
                     /gene="nagE"
                     /locus_tag="ACHJ69_001507"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO5014523.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            13351..14151
                     /gene="nagB"
                     /locus_tag="ACHJ69_001508"
     CDS             13351..14151
                     /gene="nagB"
                     /locus_tag="ACHJ69_001508"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO5014524.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            14211..15359
                     /gene="nagA"
                     /locus_tag="ACHJ69_001509"
     CDS             14211..15359
                     /gene="nagA"
                     /locus_tag="ACHJ69_001509"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO5014525.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAISVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            15368..16588
                     /gene="nagC"
                     /locus_tag="ACHJ69_001510"
                     /gene_synonym="nagR"
     CDS             15368..16588
                     /gene="nagC"
                     /locus_tag="ACHJ69_001510"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO5014526.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            16636..17388
                     /gene="nagD"
                     /locus_tag="ACHJ69_001511"
     CDS             16636..17388
                     /gene="nagD"
                     /locus_tag="ACHJ69_001511"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO5014527.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTYGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            17644..19308
                     /gene="asnB"
                     /locus_tag="ACHJ69_001512"
     CDS             17644..19308
                     /gene="asnB"
                     /locus_tag="ACHJ69_001512"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO5014528.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILTLYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            19689..19765
                     /locus_tag="ACHJ69_001513"
     tRNA            19689..19765
                     /locus_tag="ACHJ69_001513"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19723..19725,aa:Met,seq:cat)
     gene            19776..19860
                     /locus_tag="ACHJ69_001514"
     tRNA            19776..19860
                     /locus_tag="ACHJ69_001514"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19810..19812,aa:Leu,seq:tag)
     gene            19884..19958
                     /locus_tag="ACHJ69_001515"
     tRNA            19884..19958
                     /locus_tag="ACHJ69_001515"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19916..19918,aa:Gln,seq:ttg)
     gene            19993..20067
                     /locus_tag="ACHJ69_001516"
     tRNA            19993..20067
                     /locus_tag="ACHJ69_001516"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20025..20027,aa:Gln,seq:ttg)
     gene            20083..20159
                     /locus_tag="ACHJ69_001517"
     tRNA            20083..20159
                     /locus_tag="ACHJ69_001517"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20117..20119,aa:Met,seq:cat)
     gene            20208..20282
                     /locus_tag="ACHJ69_001518"
     tRNA            20208..20282
                     /locus_tag="ACHJ69_001518"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20240..20242,aa:Gln,seq:ctg)
     gene            20320..20394
                     /locus_tag="ACHJ69_001519"
     tRNA            20320..20394
                     /locus_tag="ACHJ69_001519"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20352..20354,aa:Gln,seq:ctg)
     gene            complement(20548..21723)
                     /gene="ubiF"
                     /locus_tag="ACHJ69_001520"
     CDS             complement(20548..21723)
                     /gene="ubiF"
                     /locus_tag="ACHJ69_001520"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO5014529.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVMVIEHAQPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRHNDLQELELKGGETILAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLIRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFMMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRKALKYALGL"
     gene            21869..23293
                     /gene="miaB"
                     /locus_tag="ACHJ69_001521"
     CDS             21869..23293
                     /gene="miaB"
                     /locus_tag="ACHJ69_001521"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO5014530.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGNRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTDDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLDTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            23446..24486
                     /gene="ybeZ"
                     /locus_tag="ACHJ69_001522"
     CDS             23446..24486
                     /gene="ybeZ"
                     /locus_tag="ACHJ69_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO5014531.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            24483..24950
                     /gene="ybeY"
                     /locus_tag="ACHJ69_001523"
     CDS             24483..24950
                     /gene="ybeY"
                     /locus_tag="ACHJ69_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO5014532.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQSK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEAIETEIMLALGYEDPYIAEKE"
     gene            25040..25918
                     /gene="corC"
                     /locus_tag="ACHJ69_001524"
     CDS             25040..25918
                     /gene="corC"
                     /locus_tag="ACHJ69_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO5014533.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            25943..27484
                     /gene="lnt"
                     /locus_tag="ACHJ69_001525"
     CDS             25943..27484
                     /gene="lnt"
                     /locus_tag="ACHJ69_001525"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO5014534.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVTVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSLNSIQLTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRKR"
     gene            complement(27616..29493)
                     /locus_tag="ACHJ69_001526"
                     /pseudo
     CDS             complement(27616..29493)
                     /locus_tag="ACHJ69_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
     gene            29947..30855
                     /gene="gltI"
                     /locus_tag="ACHJ69_001527"
     CDS             29947..30855
                     /gene="gltI"
                     /locus_tag="ACHJ69_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO5014535.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNAERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            31025..31765
                     /gene="gltJ"
                     /locus_tag="ACHJ69_001528"
     CDS             31025..31765
                     /gene="gltJ"
                     /locus_tag="ACHJ69_001528"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO5014536.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLSGNMGGK"
     gene            31765..32439
                     /gene="gltK"
                     /locus_tag="ACHJ69_001529"
     CDS             31765..32439
                     /gene="gltK"
                     /locus_tag="ACHJ69_001529"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO5014537.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            32439..33164
                     /gene="gltL"
                     /locus_tag="ACHJ69_001530"
     CDS             32439..33164
                     /gene="gltL"
                     /locus_tag="ACHJ69_001530"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO5014538.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRNKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            33282..34217
                     /gene="rihA"
                     /locus_tag="ACHJ69_001531"
     CDS             33282..34217
                     /gene="rihA"
                     /locus_tag="ACHJ69_001531"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO5014539.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVASGAVKPLMRNLIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESEEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(34262..34744)
                     /gene="ybeL"
                     /locus_tag="ACHJ69_001532"
     CDS             complement(34262..34744)
                     /gene="ybeL"
                     /locus_tag="ACHJ69_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO5014540.1"
                     /translation="MNKVAQHYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            34979..37561
                     /gene="leuS"
                     /locus_tag="ACHJ69_001533"
     CDS             34979..37561
                     /gene="leuS"
                     /locus_tag="ACHJ69_001533"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO5014541.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            37576..38157
                     /gene="lptE"
                     /locus_tag="ACHJ69_001534"
     CDS             37576..38157
                     /gene="lptE"
                     /locus_tag="ACHJ69_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO5014542.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            38157..39188
                     /gene="holA"
                     /locus_tag="ACHJ69_001535"
     CDS             38157..39188
                     /gene="holA"
                     /locus_tag="ACHJ69_001535"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO5014543.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQQQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            39190..39831
                     /gene="nadD"
                     /locus_tag="ACHJ69_001536"
     CDS             39190..39831
                     /gene="nadD"
                     /locus_tag="ACHJ69_001536"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO5014544.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            39855..40466
                     /gene="cobC"
                     /locus_tag="ACHJ69_001537"
     CDS             39855..40466
                     /gene="cobC"
                     /locus_tag="ACHJ69_001537"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO5014545.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARLVLSDRQHPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILVVSHQGVLSLLITRL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            40726..41043
                     /gene="rsfS"
                     /locus_tag="ACHJ69_001538"
     CDS             40726..41043
                     /gene="rsfS"
                     /locus_tag="ACHJ69_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO5014546.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            41047..41514
                     /gene="rlmH"
                     /locus_tag="ACHJ69_001539"
     CDS             41047..41514
                     /gene="rlmH"
                     /locus_tag="ACHJ69_001539"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO5014547.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDIPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            41545..43446
                     /gene="mrdA"
                     /locus_tag="ACHJ69_001540"
     CDS             41545..43446
                     /gene="mrdA"
                     /locus_tag="ACHJ69_001540"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO5014548.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            43449..44561
                     /gene="mrdB"
                     /locus_tag="ACHJ69_001541"
                     /gene_synonym="rodA"
     CDS             43449..44561
                     /gene="mrdB"
                     /locus_tag="ACHJ69_001541"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO5014549.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            44572..45660
                     /gene="rlpA"
                     /locus_tag="ACHJ69_001542"
     CDS             44572..45660
                     /gene="rlpA"
                     /locus_tag="ACHJ69_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO5014550.1"
                     /translation="MRKQWLGICIAAGMLTACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVPVPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            45800..47011
                     /gene="dacA"
                     /locus_tag="ACHJ69_001543"
     CDS             45800..47011
                     /gene="dacA"
                     /locus_tag="ACHJ69_001543"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO5014551.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            47121..47384
                     /gene="ybeD"
                     /locus_tag="ACHJ69_001544"
     CDS             47121..47384
                     /gene="ybeD"
                     /locus_tag="ACHJ69_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO5014552.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            47485..48126
                     /gene="lipB"
                     /locus_tag="ACHJ69_001545"
     CDS             47485..48126
                     /gene="lipB"
                     /locus_tag="ACHJ69_001545"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO5014553.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPLIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT        11705 a        12602 c        12381 g        11612 t
ORIGIN      
        1 tgcgaagcgg aattaattcg cgaagaaaag cagaaaaaac gccgcagagc gcggcgtttg
       61 ggataatttg catatttctt gccggatgcg gcgtaaacgc tctatccgac aacgtaaact
      121 tactcgccta ctttcgccca ggtatcacgc agcccaacgg tgcggttaaa taccggtttt
      181 tccgccgtag aatggcggct gtcgaggcag aagtaacctt cacgctcaaa ctggaatgct
      241 ttacccgcaa ccgcatcttt cagcgacggt tcagcaaagc cctgtttgat caccagcgac
      301 tccgggttaa tcaccgacag gaaatcatcc gcagcaccag ggttcggcac gctgaacaga
      361 cgatcgtaca ggcggatttc aaccggcagc gcatgtgccg cgctcaccca gtgaataaca
      421 cctttgactt tacgaccatc tgccggatct ttgcttaagg tgtcggcgtc ataagtacag
      481 aagatggtgg tgatattgcc ttcggcatct ttctccacgc gttctgcctt aatgacgtaa
      541 gcattacgca gacgcacttc tttacccagc accagacgtt tgtactgctt gttagcttct
      601 tcgcggaaat cggcgcgatc gatccaaatc tcaccgctaa atggcacctg acggctgccc
      661 atttccggtt tattcggatg gttcggcatg gtaaccattt cgccttcgcc ctggtagttt
      721 tcgataacca gtttcaccgg atcgataacc gccattgcgc gcggcgcatt ttcgttgaga
      781 tcttcacgga tgcaggattc cagtgacgcc atctcaatgg tgttgtcctg cttggtcaca
      841 ccaatgcgtt tgcagaactc acgaatagaa gccgcagtgt aaccacgacg acgcagacca
      901 gaaatggtcg gcatacgcgg gtcatcccag ccttcaacgt gcttgtcggt caccagcagg
      961 ttcagcttac gcttggacat cacagtgtat tccagattca ggcgcgagaa ttcgtactgg
     1021 cgcgggtgaa caggaatggt gatgttgtcc agtacccagt cgtacagacg gcggttgtcc
     1081 tggaactcaa gcgtacacag agagtgcgta ataccttcca gcgcatcgct gatgcagtgg
     1141 gtgaaatcgt acatcgggta gatgcaccac ttgttgccag tctggtggtg ttcagcaaac
     1201 ttaatgcggt acagcaccgg atcgcgcatc acgataaacg gcgaagccat gtcgatcttc
     1261 gcacgcaggc aggctttacc ttcttcaaaa ccgccggtac gcattttctc gaacagcgcc
     1321 aggttctctt caacgctgcg atcgcggtac gggctgtttt tacccggctg cgtcaaggtg
     1381 ccgcggtatt cgcggatctg ttccggcgtc agttcatcaa cgtacgccag gcctttattg
     1441 atcagttcga tcgcataggc gtggagctga tcaaaataat cggaggagta acggacgtta
     1501 ccagaccagt ggaagcctaa ccactctacg tcgtttttga tcgaatcgac atactcgata
     1561 tcttctttta ccgggttagt gtcgtcgaaa cgcaggttgc actggccttt atagtcctgg
     1621 gcgatcccga agttcaggca gatagatttc gcatggccaa tatgcagata gccattcggc
     1681 tccggcggga aacgggtgtg tactgtggtg tgcttaccac tggccagatc ttcatcgatg
     1741 atctgacgaa taaagttagt cgggcgggct tctgcctcac tcatcgtgga ttcctcaaag
     1801 cgtaaacaac gtataacggc gtatgatctt ataagcggga caggctgaca actgttagtt
     1861 agcgaaaaaa tggttttgcg taagatattg gggcgttttc tgcgctactg ccagcagatc
     1921 ctcgcgctgg aaacgtaagg ccaaacgtcg ccagcctgac gcatttatta aattgccgga
     1981 tgagctggcc gcagagtcat agattagtcg gctatgatta aacgcgtcga caaggacgca
     2041 agcgttctac gcgtctgccc atcaagctga ctatccgtaa taatctctgt cagttcatta
     2101 agatgcgcga tattaaacag cgaccatgaa ccgtattttg agctgtcagc caggagaatt
     2161 ttgcgcgaag catgcgcgat aagatcgcgt tttagtgcgg ctttctcttc cgtgggtgct
     2221 gtaataccct tttcaagatc ccagctgtta cagcttaaaa atgccacgtc cggccaggta
     2281 ttttgcagca atttacgacc atgttcgcca atgcaggact ggctggagtc gtcgatacgt
     2341 ccgccgataa tcgtcacttc aatttgcttg aattcagcca aaaaaagcgc gatatgcagg
     2401 tcgcttgtga tcacgcgaag cggtaaatgt gtaatctgcc gcgcaagttc aatcattgtc
     2461 gtaccggcat caaggacgac ggaatccccc gctttgacca ttgacgccgc tgcgctggcg
     2521 atggcatgtt tttccgccag gctgcgctgc attttctcat gggtagtcgg ttgggtagga
     2581 ataaaccggt tgagggtcac accgccatgt gtacggctga taacaccctg ctcatcgagt
     2641 tttatcaggt ctcgacgaat cgtagccggc gacgcattgg ttaccgccac cagctcatca
     2701 acggtgacca gattatggct tttcaggtaa tccatgatct gctctaaccg gttatatccc
     2761 ttcattttat tctcgcgaaa tctgcattgc taactggatg gaaactgcca tgctctcaga
     2821 cttggctttt cctgtccagg caatatcaaa cgcggtgccg tggtcggcag aagtgcgaat
     2881 aaatggcaac ccggcggtga tattcacccc atcataaaat ccaagcagtt ttaaagggat
     2941 atgcccctga tcgtggtaca tagccactac catatcgaat agcccttcat ggcattgcat
     3001 aaacacggta tctggcgggc acgggccagt aacatcaata tcttcggcct gcattgcttc
     3061 aatcgcaggc gcgataatct cgatttcctc agtgccgaat aaaccatgtt cccctgcatg
     3121 tggatttaca cccgccacgg caatacgcgg acgttcaata ccaacacgtt tcaggaagtg
     3181 gttcgcaaca cgaataaccg ttttgaccct ttcaccattc aaggtatcca ggaatttacg
     3241 cagtgagatg tgagtcgaaa tatggataac cttgagctta tcggtataga gcaccatcgc
     3301 gtactctttg ctgccggtaa gatgggccag caattccgta tgccccggat agttatgccc
     3361 gccaagatgc agcgcttctt tattcagcgg cgcagtggct atggctttca cctcgcctga
     3421 taaggccaga gccgttgcac gtttgataca gcgatacgct aaatcgcctg ccgcagcctg
     3481 taccactccg ggtgtcagcg catcggggtt agccagcggc tcatccatca cgttaatcac
     3541 gccgggagca aaatgggcgt cgcacacctt ttgaatgacg cgtaactctg cctgcggcgt
     3601 gatattcatc gccataatac gtcgcagcgt gcgcacacag cccaccacca ccaccggggc
     3661 accagcaagc ccggcttcgc tcagggattt aataatgatc tccgggccaa tacccgccgg
     3721 gtcacccatc gttaccgcaa taatattatt cactacactt ctcctcgata aaattcagaa
     3781 cctgatgcaa cgtggtctca tcgccaaacc caccggcttt cgtcatcacc gaacgggacc
     3841 agacaccgcc cagaaagtga ccgtatggaa cgcattgcgc caccttcccc gtaatacgaa
     3901 atccggtcgc gcctaacgca ctggctgttg ccattgcgac atcaccacca gagagataaa
     3961 gcgcatcggg cattgtgcga agcagaactt gccgggtaag ttcgccaaga aaccgacaaa
     4021 ttttttcccc aagtgcagcg cgactcatct gccaacgctg gcaaagggtg tcgatctgat
     4081 gccttgctac agaatcgtta caggtgtgga caatgcagtg gtcgcctttc tgcaaggcct
     4141 gcacaatacg cgcgtcatag tcactggcat tgcctgccag aatgtgttca acatcgacgt
     4201 atatctgtgt gatacatggg tgtgaatgaa gtgtggcgat ctgccgttga gcgatttcgc
     4261 tcattgaacc aataatggcc agcacggtgg gtggcggcat aatcgcggag cgccgggcaa
     4321 cggcatcaca aataccagcg gagccgacca gcagcagcgg ttgtttagcc tgcatcacag
     4381 cggcagcgat gcgatccagg tcgctgtccg tctgcgcatc tatcacgcta atccacggtt
     4441 gctcataaga caaatggctg agctgagaaa ccgtaacggg ctggcatgga atggccgtct
     4501 gcaaccggat gatgtccgca attcgggcgc tcaataccgg ggttttcgga tcgctggcaa
     4561 actcggtttc cgttatccgc acaccgttta cccaacactc accggccact gtggtccttc
     4621 ccgcctgagg aaatgcgggg gcaaccactg cgcccgttat accgcatgcc ttcatcaggg
     4681 cttcaatttc tgcgccgata ttgcctcgca gcgtggaatc tatcttttta atcacgagcg
     4741 gcggattatt cgccagaggg agatgactca tcagcgaggt tattttcatc gccgccagct
     4801 taggatccat cgcccgactg tcgctgttaa tcacccatac gctggcatca ccgcgataag
     4861 gcatttcaaa ggccacatca actgtatgcc ccacctgcgc caggcttacc cccgcgtcgt
     4921 tggctcccgt gaaatcatcg gcaacgacga gaatgctcgg cttagtgatt cccttttcca
     4981 tcatcactcc aggttgtttt atgataacaa tgattatatt caatcatgat tgattaaatg
     5041 cgagcaatct caagttatta atcactcgaa taaattataa aattagacca tgttacgtag
     5101 gctacggcat tttgaatgat tgaattcaat caccagaaga gaggttattt gtggtaatta
     5161 tcaatagcac gatactgagc ggcgcaggcg ctatcccttc cctgacgtcg ctcttacccg
     5221 acatcagaaa aatgctgctg gtcactgacc gtaatattgc gcagctcgac ggtgtgcagc
     5281 agattcgcgc cttactggaa aagcactgcc cgcaggttaa cgttatcgat aatgtgcccg
     5341 cagagcccac gcatcatgat gtgcgccagc taatggatgt ccttggcgat gcctcttttg
     5401 atgtggtggt cgggatcggc ggtggcagcg tgttggatgt ggcaaagctg ctatcggtgc
     5461 tttgccatcc acaatctccg gggctggatg cgctgcttgc gggtgaaaaa ccgactcagc
     5521 gggtgcaatc atggttgatt cctacaaccg ccggaaccgg ctcagaagcc acgccgaatg
     5581 cgattctggc aatccctgag caaagcacga aggtgggtat tatttcccag gtgctgttac
     5641 cagactatgt ggcgcttttc ccggaactga ccaccagcat gcccgcgcat attgcggcgt
     5701 ccacgggcat tgatgctctt tgccacttac tggagtgttt taccgcgacc gtggcaaatc
     5761 cggtcagcga taacgcggcg ctgactgggt taagtaaact tttccggcac attcaacccg
     5821 ccgtgaacga tcctcaggat ctgcgcgcaa aactggaaat gctgtgggcg tcttactatg
     5881 gcggcgtagc gataacccat gcgggcacgc atctcgttca tgcgctctcc tacccgttag
     5941 gtggcaaata tcatctgccg catggcgtcg cgaatgccat cttgctggcg ccgtgcatgg
     6001 cgtttgttcg cccctgggcg gtcgagaaat ttgcccgggt ctgggattgc attcccgatg
     6061 cggaaaccgc cctgagcacg gaagaaaaat ctcatgccct ggtgacctgg ttacaggcat
     6121 tagtccatca actcaagcta cccaacaatc tcgcggatct cggcgtaccg ccagaggata
     6181 ttgcctctct tagcgaggtg gcactgaacg tgaagcgcct tatgaacaat gtgccgtgcc
     6241 agcttgatct acaggacgta caggccattt accaaacact gtttccgcaa catcaattta
     6301 aggaataaat catgccgaaa accattgaag gtgtattaac cgctatcgtc accccgttta
     6361 ccgcgaccgg cagtctgaat atcccggcgt taaaagtgca ggtgaatcgt caactggaag
     6421 cgggtaacgc gattttttgc ggcggcacca acggcgagtt ttttgtgttg aatgaacagg
     6481 agaagctcag cgttacgcaa acctgcgtgg atgaagtggc tggtcgtgcg ccggtcgtgg
     6541 cacacattgg cgagatctct acgcgcgaaa cgattcgtct gggtcagcag attgaaaaac
     6601 ttggcgtaga tgccgtgtcg gtcattgccc cttacttcgt accgctgaaa caggaagagt
     6661 tgattgcgca ttacagcgca attgccgacg cactgagcat tccgatgttt ctctataaca
     6721 tcccggcccg cactggcaat acgatccagc cagaaaccgc gcgtgctctg gcgtcgcacc
     6781 ccaatatcat tgggattaaa gacagcgccg gaagcgacga aagcctgaag ggttttctcg
     6841 atgcggtgcg cgacattgac ggctttaatg tgctcaacgg cccggattcg ctgatccata
     6901 aagggtttgt cgagggatgt tcaacctgta tctctgggct cgccaacatc gcgccgcgag
     6961 aaatcaatgc catctggtct cgcttccatg ccggtgatat tgaagggtct tatgaagcac
     7021 aggagagtgt aacgggcctg cgtaccgatc tttataaagt agcattctcc ccggctgcgg
     7081 tgaaaaaagc gttgcagctt atggggcatg atgttggtga tagccgctac gcggttaatt
     7141 tcacgcctga acagatcagc gagattcaac aaattattcg tcattataac attaattaaa
     7201 ttaactctat tatccagccc gctataaaat ttagataaag agaataaagg attaccccat
     7261 gtttttgtgg tttatgcagc cagatatctg gctcacttta attaccctca cctttctgga
     7321 aattattctt ggggtggata atattatttt tctttcactg gtggtggcaa aacttccgtt
     7381 atcacaacaa aatatggcgc gtaaactggg tttaagctgc gcgatgatta tgcggatatt
     7441 attgctgata tccattgcgt ggctttcaca tattacccag ccgcttttta ccgtgctgac
     7501 gctggagatt tcgttccgca ccttgattct gctcgggggc gggctgttcc tgatttataa
     7561 agcgatacag gagatacgcg aagagctgtc gcccgcgcag gaagacgaac atggcagcgg
     7621 atcgcggcaa ctgtcgttgt gcggtgccat tgtacagata atgctgctcg atatcgtgtt
     7681 tagcctggat tcggtgatca ccgccgtcgg cctttctcag catatattta tcatgattgc
     7741 cgccgtaatg attgccgtcg gtgtcatgat gtttgcggcg aaaacaattg gtgatttcgt
     7801 taacgccacg ccgtcaataa aaatactggc gttaaccttt ttattgtttg tcggtgtatt
     7861 gctggttgcc gatagtctca acattcatat tgccaaagag tatctctact tcgcgatatt
     7921 tttctcatta agtgttgaga cactcaatat tatccgtgaa agaaaaaata aaaaagtttc
     7981 gacattgatc gaaacgcatg aatcgcactc gcattaataa atctattacc aactttaaga
     8041 tggagtctaa ctatgtctgt agcccttatt cgtgacggcg tcatccgtcc atccgaaaac
     8101 gattcgcatg tgcttaccgc catgctgccc tcgtcctgcc cgcaaaacca cgcggcgaat
     8161 attctgcctt tgccggatgg cgcgttaatg tgcgtctggt ttgcgggaac gcaggaaggt
     8221 attgccgata tttctgtctg gggatcgcga ttgcccgctg gcggaatgca gtggagcgat
     8281 gcggtcaaac tttcccacga tgacacgcgt tcagagcaaa acccggtgct gtttctcgcg
     8341 ccggataacg tgctgtggct cctatggacc gcacaaatat ccggtaatca ggacaccgcc
     8401 atcgtccgct accgtaagtc agacgacctc ggacaaacct ggggcgagat tgccaccctg
     8461 cttgataaac ccggtacgtt cattcgacag ccaatcacgg tgcttgataa cggcaactgg
     8521 ctgctgcccg tcttttattg ccgtacccaa cccggcgaaa aatgggtcgg caatgatgat
     8581 atcagcgcag tgaaaatttc cgctgacggt ggtcactcct ggcgcgatgt cgaggtgccg
     8641 caaagtcttg gctgtgttca tatgaatatc acaatgctgc acaacggtac gttggttgct
     8701 ttctttcgca gccgttgggc agacaacatc tatatcagcc attccgcgga caacggcgag
     8761 agttggtcgg tgccgcaagc aaccgaattg cccaacaaca attcgtctat tcaggtaacg
     8821 acgcttgcca gcggcgaact ggcgctggtg tacaacgcga tgagtgcggc aggcgcagta
     8881 gaacatcgcg cgtcgcttta tgacgaaatc gacgatggcg acgacagccg caaagaaccg
     8941 acagccgtcg gacgctccgc tttctggggt gccccacgcg cgccgatgac ggtagcgatt
     9001 tctgccgatg gagggaaaag ctggccgtgg cggcgcaatc tcgatgaagg cgacggctac
     9061 tgcatgacca acaattccct ggaaaagcta aatcgtgagt tctcctaccc cagcatcaaa
     9121 caaagcccgg acggtacgct acatatcgct tacacttggt ggcgtcaggc catcaagtac
     9181 gtacgcatct cccccgagtg ggttaagggg caggcatcat gattatcggt aatctcaacc
     9241 atttgtcgct ggcggggctt cctgcctggg tgagaaatat tctgctgcgc ccggagtgtt
     9301 cgttatcggc gttatccacc agagaggacg gtcgctggca acctgaagga tgtcggtggt
     9361 tctgcacgct gggaacttca gacacgcaac ccgccgaact ccgccatacg gagtatcacc
     9421 atctatgggc cgatatacag gtggtgatta ctggttgtga gggcattaat gcgggaacgc
     9481 ggcccatcgc tcgcgaaaac gatgaagaac gtaagcccga tctgtttatc gccccatccc
     9541 ctgaaaacag tgtcgccatc accctacacg ccggtgattt cgccgtcttt atgcctgagg
     9601 aacctcacca ggcgctgtgc gcaattggca cggctacttc aatacgcaaa gcggtcttta
     9661 aagtaccgcg cgacatgctg gaagcctaaa aggagagaaa aaatgacaat ggaaatgatc
     9721 ctggcattag gaatactggt cctgatgatc gtgctgatca tgtcggacaa aatgcctttt
     9781 ggcgcaccgc cactgcttgc gtgtttattg ctggttgtat cgggactttc gaccgttcaa
     9841 caggcttttg ccggattcgt caatccaagc gtggtgatga ttgcagggtt tatggtggtc
     9901 atggcggcgc tacaaaaaac ccggcttatc agcaacgtta aatctgcgat gataagcctg
     9961 gtcaataaag gcagttaccg cagctatggg ctgttgttag tcattgttat gctgggtgcc
    10021 agtcttgccg gcaccggagc gaccggttat tacgtgctaa ttctgtcgct ggtatccacc
    10081 attccgtaca gtaaaaagct accaacatcg aaattaatga tgcccctggg atttgccacc
    10141 aaccacccgc ttcttcctat caatctcgcg cttctctttg gcgttacggc gaccgtactc
    10201 gaaaccgcgg ggtttcatca ggagatttcg atggggcgct ttgcgctggt gaatctcatc
    10261 atgtcagcgg ctttcctcgc atggagtctt attgcctacc gttttcttcc ggatcatccc
    10321 attgcggatg cttccgagga tgcgctggcg gcacgcgagg aaacgtttaa cgcccttcct
    10381 gcctggaaag aatattgcac tattgccgca ttcgctgtca gcgttatcgg catgatgcta
    10441 atgaacattc tgggaaatat cgccttcgtt attccgggtc ttgcgggtgc gtttgttctg
    10501 atgatcgatg tactggactt caaagaagtt cgcgatcata tgggggctcc ggtcatttta
    10561 atgatggcgg gggtgatcgg tgtagctgat gcactggcgg gcaccggctt tacggcgatg
    10621 gttggcgatg ccgttgcagg tgtgctcggt tctggcgtct caccgtttgt atttattgtc
    10681 gcctttgctt tgcttaccag tacctgcgcc accttcacgg gttcgaatat ggggtcagta
    10741 tatatttttg cccccatcgc cattgccgcc tgtactagcc ttgggcttaa cccgaccgct
    10801 gccgcgattg cggtggtgat ttctggctgg aatggcggct atatgcctat tgacggaatt
    10861 ccagccatga tcctcggaat ggggaaatac aaacttcccg aattctgggt gttctctgta
    10921 ccaatgtatc tcatccgcat tctggcgctt tgtgcaggcg ctgtttttat cttcccaatg
    10981 taatctcaga caaaaaaagc ggcagagaag atccctgccg ctttttattg ttggatgcga
    11041 cgctgccgcg tcgcatcaga cataaagcag attacttttt gatttcatac agcggtgttt
    11101 gacccgccac aacatggccc tgagctttaa tgataaggcc gctgaaatcg tcgatattgc
    11161 tgcaaaccac cgggctaatc atcgagcgag cgttagcgtt caggtaatcc agatccattt
    11221 ccagaatcgg ttgccctgcg cttacctgcg cgccctcttc caccagacgt ttaaagcctt
    11281 taccttccag cgctacggtg tcgataccca tatggacgac gatctccgcg cctttttcgg
    11341 tttccagaca gaatgcgtgg ttggtgttga agattttcac gattgttcct gcggctggtg
    11401 atacgacgat tttatctgtc ggtttcaccg ccacaccgtc acccaccgct ttgctggcga
    11461 atgcttcgtc aggaacctga tccagtgcca caacatcccc ggtaatcggg gataccagct
    11521 ccgcgataga taccgcgttt ggtacagcct gcggttttgc tacaggagcg gcagttgccg
    11581 gagttgcttc agctgacgca gcggctaccg gaccacgggc aacgactttc ttcatcgcat
    11641 cgccgatgga ttctgctttc gcgccaacaa tcacctgaat agtctgtttg ttcagtttca
    11701 ctaccccaga agcacccaga cgtttacaca tcgcatcgtt gacgcgagct gagtcaacca
    11761 cggtaaggcg cagacgagta atacaggcat caatggcttt caggttgtca gtaccgccaa
    11821 ccgcagcaat atagttggtt gccagttgat tgagaccttc ttcagtattg ctgttagcct
    11881 cttcagtaac gatctcgtct tctttatctt cacgacccgg cgttttcagg ttgaacatgc
    11941 ggataaccaa actgaacacc acgaagtaga tagcgaagaa gacaaccccc atcaccagca
    12001 gcatccagac gttctggctg gcggccggca gattatacat caacgcgtag tcgatagccc
    12061 ccgcagagaa agagaagccg gcatggatac ccagcaacgt tgccacaaac aggctgatac
    12121 cggtcagcag tgcgtgcagg aggtacagca gcggtgcaag gaacatgaac aggaattcca
    12181 gcggctcagt cacaccggtc aggaacgcag taacagcaac agaaagcagc atcccgccaa
    12241 ccatcggacg acgctctttc ggtgctgcga agtacatcgc cagcgccgca cccggcagac
    12301 cgaacatcat aatcgggaag aagccggaca tgaacatccc cgcggtgccg tcaccggcat
    12361 agaagcggtt gatatcaccg tggaaaaccg ttcccgccgc gttggtgaat tcaccaatct
    12421 ggaaccaggc gatggtgttc agcacctgat gcagaccggt tgggatcagc agacggttga
    12481 tgaaaccaaa gataccggaa cccagcgcgc ccgcagaaac gatccactcg ccgcctgcat
    12541 ggatagcgtg ctgtaccggc ggccagacgt aaccaaaaat ggccgccagc accagacaga
    12601 agaagccggt ggcgatcggc acaaagcgtt tgccgccgaa gaagctcagg aagtccggca
    12661 gtttaatatc ggaccaacgg ttataggctg cgccaccaac cagaccggta atgatacccg
    12721 ccagtacacc catgttaatt tctgggttga tggtcaccat cgctttggtt aacacaaagt
    12781 aacctaccgc acccgccagt gccgccgcac ctgcgctgtc tttcgaccag ctggatgcca
    12841 cgccgatggc gaagattaat gcgaggttat caaaaatcgc accgcccgcc tgggcaataa
    12901 acgcaacgtt aagtaaatct ggctgaccga atcgcagcaa cagcgccgcc accggcagca
    12961 ccgcgatagg gagctgtaac gccctaccga gtcgctggaa aaaacctaaa atattcatct
    13021 tattccccct acgagaaccc tatttggctc gtttaaagcc gtatttttat tttgctgcaa
    13081 attgtactgc cgatgttctg taatcagatt gttagatcat ctgctacaga gtgtgtgaaa
    13141 atttaattcg tatcgcaaat taaacgcgtg tcttttgtga gttttgtcac caaatatcgt
    13201 tatcatcact cccttttact ggctaaacca gaaaacttat tttatcattc aaaaaatcag
    13261 gtcggattga cgcctgtctg cgcaaatcca ggttacgctt aaagatgcct aatccgccaa
    13321 cggcttacat tttacttatt gaggtgaata atgagactga tccccctgac taccgctgaa
    13381 caggtcggca aatgggctgc tcgccatatc gtcaatcgta tcaatgcgtt caaaccgact
    13441 gccgatcgtc cgtttgtact gggcctgccg actggcggca cgccgatgac cacctataaa
    13501 gcgttagtcg aaatgcataa agcaggccag gtcagcttta agcacgttgt caccttcaac
    13561 atggacgaat atgtcggtct gccgaaagag catccggaaa gctactacag ctttatgcac
    13621 cgtaatttct tcgatcacgt tgatattcca gcagaaaata tcaaccttct caacggcaac
    13681 gccccggata tcgacgccga atgccgccag tatgaagaaa aaatccgttc ttacggaaaa
    13741 attcatctgt ttatgggcgg tgtaggtaac gacggtcata ttgcatttaa cgaaccggcg
    13801 tcttctctgg cttctcgtac tcgtatcaaa accctgactc atgacactcg cgtcgcgaac
    13861 tctcgtttct ttgataacga tgttaatcag gtgccaaaat atgccctgac tgtcggtgtt
    13921 ggtacattgc tggatgccga agaagtgatg attctggtgc tgggtagtca gaaagcactg
    13981 gcactgcagg ccgccgttga aggttgcgtg aaccacatgt ggaccatcag ttgtctgcaa
    14041 ctgcatccaa aagcgatcat ggtgtgcgat gaaccttcca ccatggagct gaaagttaag
    14101 actttaagat atttcaatga attagaagca gaaaatatca agggtctgta attgttatcc
    14161 ctgccctggc tccttgctca gggcattatt ttttaaaatc gggggtcaga atgtatgcat
    14221 taacccaggg ccggatcttt accggccacg aatttcttga tgaccacgcg gttgttatcg
    14281 ctgatggcct gattaaaagc gtctgtccgg tagcggaact gccgccagag atcgaacaac
    14341 gttcactgaa cggggccatt ctctcccccg gttttatcga tgtgcagtta aacggctgcg
    14401 gcggcgtaca gtttaacgac accgctgaag cggtcagcgt ggaaacgctg gaaatcatgc
    14461 agaaagccaa tgagaaatca ggctgtacta actatctgcc gacgcttatc accaccagcg
    14521 atgagctgat gaaacagggc gtgcgcgtta tgcgcgagta cctggcaaaa catccgaacc
    14581 aggcgttagg tctgcatctg gaaggtccgt ggctgaatct ggtaaaaaaa ggcacccata
    14641 atccgaattt tgtgcgtaag cctgatgccg cgctggtcga tttcctgtgt gagaacgccg
    14701 acgtgattac caaagtgact ctggcaccgg aaatggttcc agcggaagtc atcagcaaac
    14761 tggcaaatgc cgggattgtg gtttctgctg gtcactccaa cgcgacgttg aaagaagcga
    14821 aagccggttt ccgcgcgggg attacctttg ccacccatct gtacaacgcg atgccgtata
    14881 ttaccggtcg tgaaccgggc ctggcgggtg cgatcctcga cgaagctgac atttattgcg
    14941 gtattatcgc tgatggcctg catgttgatt acgccaacat tcgtaacgct aaacgtctga
    15001 aaggcgacaa attgtgtctg gttaccgatg ccaccgcgcc agcaggtgcc aacattgaac
    15061 agttcatttt tgcgggtaaa acaatatact accgtaatgg actttgtgtg gatgagaacg
    15121 gtacgttaag cggttcatcc ttaaccatga ttgaaggcgt gcgtaatctg gtcgaacatt
    15181 gtggtatcgc actggatgaa gtgctgcgta tggcgacgct ctatccggca cgtgcgatta
    15241 gcgttgagaa acgtctcggc acgctcgccg caggtaaagt agccaacctg actgcattca
    15301 cacctgattt taaaatcacc aggaccatcg ttaacggtaa cgaggtcgta actcaataag
    15361 agaaagtatg acaccaggcg gacaagctca gataggtaat gttgatctcg taaaacagct
    15421 taacagcgcg gcggtttatc gcctgattga ccagtacggg ccaatctcgc ggattcagat
    15481 tgccgagcaa agtcagcttg cccccgccag cgtaaccaaa attacgcgtc agcttatcga
    15541 acgcgggctg atcaaagaag ttgatcagca ggcctccacc gggggccgcc gcgctatctc
    15601 catcgtcacc gaaacccgca atttccacgc aatcggcgta cggcttggtc gtcacgacgc
    15661 caccatcacc ttgtttgatc tcagcagcaa agtgctggca gaagaacatt acccgctgcc
    15721 ggaacgtacc cagcagacgc tggaacatgc cctgctgaat gccattgctc agtttattga
    15781 tagctaccag cgcaagctac gcgagctgat cgcgatttcg gtgatcctgc cagggcttgt
    15841 agacccggac agcggcaaaa ttcattacat gccgcatatt caggtagaaa actgggggct
    15901 ggttgaagca ctggaagagc gttttaaagt gacctgtttt gtcggccacg atatccgcag
    15961 tctggcgctg gcagagcact acttcggtgc aagtcaggat tgcgaagact ccattctggt
    16021 gcgtgtccat cgcggaaccg gggccgggat tatctctaac gggcgaattt ttatcgggcg
    16081 caacggcaac gtcggtgaaa ttggccatat tcaggtcgaa ccgctgggtg aacgctgcca
    16141 ctgcggcaac tttggctgtc tggaaactat cgccgccaat gctgccattg aacaacgggt
    16201 gttgaatctg ttaaagcagg gctaccagag ccgtgtgcca ctggacgact gcaccatcaa
    16261 aactatctgc aaagccgcga acaaaggcga tagcctggcc tcggaagtga ttgaatatgt
    16321 cggtcgtcat ctgggtaaaa ccatcgccat tgctatcaac ttatttaatc cgcaaaaaat
    16381 tgttattgct ggagaaatca ccgaagccga taaagtgctg ctccctgcta ttgaaagctg
    16441 cattaatacc caggcgctga aggcgtttcg tactaatcta ccggtggtac gttctgagtt
    16501 ggatcaccgc tcggcaatcg gcgcttttgc gctggtaaaa cgcgccatgc tcaacggtat
    16561 tttgctccag catttgctgg aaaattaatg tgcttttata gtggcgctta ttgttgtcaa
    16621 tattctgggt agtccatgac cattaaaaat gtaatttgcg atatcgacgg cgtgctgatg
    16681 cacgataacg tcgccgtacc cggtgcagcg gaatttttgc acgggattat ggataaaggc
    16741 ctgccgctgg tgttgctgac caactatcct tcacagaccg ggcaagatct ggcgaatcgc
    16801 tttgccactg ccggtgtcga tgtaccggat agcgtgtttt atacctccgc gatggcgacc
    16861 gccgatttcc tgcgtcgcca ggaaggcaag aaagcgtatg tggtgggcga aggcgcactg
    16921 attcatgaac tgtacaaagc cggtttcact attaccgatg tgaaccctga ttttgtgatt
    16981 gttggcgaaa cgcgttccta caactgggac atgatgcata aagcagccta tttcgtcgct
    17041 aacggtgcac gctttatcgc caccaacccg gacacctacg ggcgcggttt ttatcccgcc
    17101 tgtggcgcgt tgtgtgcagg gatcgagaaa atctccgggc gcaaaccgtt ctatgttgga
    17161 aagccgagtc cgtggatcat ccgcgcggca ttaaacaaaa tgcaggcgca ttcggaagaa
    17221 acggtgattg tcggcgataa cttgcgtacc gatattctgg cgggcttcca ggccggtctg
    17281 gagacgattc tggtgctttc tggtgtttcg tcgctcgacg atatcgacag tatgccgttc
    17341 cgcccaagtt ggatttaccc atcggtcgct gaaatcgacg ttatctgaat agcaaccacg
    17401 cctcagggcg tggtttctta tttttaccca tcattaaaat caccctattc aataaaaccc
    17461 gtcaaaaatt atcgatccat caataaatat acccaaagcc cctcctcttt ttcatcaatc
    17521 agcaatcgcc attacgtttt ttatttttcc actgccaaat cgcttgcgca ccgtgctgct
    17581 ttgcggcatt tttttaacaa gtaaacacaa cacgcagtaa acaccaggtt aacggagaag
    17641 gttatgtgtt caatttttgg cgtattcgat atcaaaacag acgcagttga gctgcgtaag
    17701 aaagcactcg agctgtcacg cctgatgcgt catcgtggcc cggactggtc cggtatttat
    17761 gccagcgata acgccatcct cgcccacgaa cgtctgtcaa ttgttgacgt taacgcgggg
    17821 gcgcaacctc tctacaacca acaaaaaact catgtgctgg cagtaaacgg tgaaatctac
    17881 aaccaccagg cattgcgcgc tgaatatggc gatcgttacc agttccagac cgggtctgac
    17941 tgtgaagtga tcctcacgct gtatcaggaa aaagggccgg aatttctcga cgacttgcag
    18001 ggcatgtttg cctttgccct gtacgacagc gaaaaagatg catatctgat tggccgcgac
    18061 catctgggga tcatcccact gtatatgggc tatgacgaac acggtcagct gtatgtggcc
    18121 tcagaaatga aagccctcgt gccagtttgc cgcacgatta aagagtttcc ggcggggagc
    18181 tatttgtgga gtcaggacgg cgaaatccgt tcttattatc atcgcgactg gttcgactac
    18241 gatgcggtga aagataacgt gaccgacaaa aacgagctgc gtcaggcact ggaagattcc
    18301 gttaaaagcc atctgatgtc tgatgtgcct tacggtgtgc tgctttctgg tggtctggat
    18361 tcctcaatta tttccgctat caccaagaag tacgccgccc gtcgcgtgga agatcaggaa
    18421 cgctctgaag cctggtggcc gcagttgcac tcctttgctg taggtctgcc gggttcaccg
    18481 gatctgaaag ccgcccagga agtggcaaac catcttggca cggtgcatca cgaaattcac
    18541 ttcactgtac aggaaggtct ggatgccatc cgcgacgtga tttaccacat cgaaacctat
    18601 gacgtgacga caatccgcgc ttcaacaccg atgtatttaa tgtcgcgtaa gatcaaggcg
    18661 atgggcatta aaatggtgct gtccggtgaa ggttctgacg aagtgtttgg cggttatcta
    18721 tacttccaca aagcgccgaa cgctaaagag ttgcatgaag agacggtgcg taaactgctg
    18781 gccctgcata tgtatgactg cgcgcgtgcc aacaaagcga tgtcagcctg gggcgtggaa
    18841 gcacgcgttc cgttcctcga caaaaaattc cttgatgtgg cgatgcgcat taacccacag
    18901 gataaaatgt gcggtaacgg caaaatggaa aaacacatcc tgcgtgaatg ttttgaagcg
    18961 tatctgcctg caagcgtggc ctggcggcaa aaagagcagt tctccgatgg cgtcggttac
    19021 agttggatcg acaccctgaa agaagtggca gcgcagcagg tttctgatca acagctggaa
    19081 actgcccgct tccgcttccc gtacaacacg ccgacctcaa aagaagcgta tctgtaccgg
    19141 gagatctttg aagaattgtt cccgcttccg agcgccgctg agtgcgtgcc gggcggtcct
    19201 tccgtcgctt gttcttccgc taaagcgatt gagtgggatg aagcgttcaa gaaaatggac
    19261 gatccgtctg gtcgcgcggt tggcgttcac cagtcggcat ataaataaga cgtgtaatat
    19321 tgccccggag aaatccgggg caatttcagt tactggggcg cttgttgatt ctgatatgca
    19381 gtaaggaaat gttccgggtt tgccacatcg atgtttgaca actcattact gtaggccagt
    19441 atttcataga ttaatttccc accactattg ttgaattccg aatttcatcc tgtatgacaa
    19501 aagaatggct gtagggtggg gaaaaaacac aaagtaaaca ataattgacg aatatagcgc
    19561 catgctgttc gcaacctaac caaacagtca ctttcgagca attttccttg aaaaagaggt
    19621 tgacgctgca aggctctata cgcataatgc gccccgcaac gccgataagg tatcgcgaaa
    19681 aaaaagatgg ctacgtagct cagttggtta gagcacatca ctcataatga tggggtcaca
    19741 ggttcgaatc ccgtcgtagc caccatcttt tttttgcggg agtggcgaaa ttggtagacg
    19801 caccagattt aggttctggc gccgcaaggt gtgcgagttc aagtctcgcc tcccgcacca
    19861 ttcaccagaa agcgttgtac ggatggggta tcgccaagcg gtaaggcacc ggtttttgat
    19921 accggcattc cctggttcga atccaggtac cccagccata ttcttcgaat aagcggttca
    19981 ccgcccggtt attggggtat cgccaagcgg taaggcaccg gtttttgata ccggcattcc
    20041 ctggttcgaa tccaggtacc ccagccatcg aagaaacaat ctggctacgt agctcagttg
    20101 gttagagcac atcactcata atgatggggt cacaggttcg aatcccgtcg tagccaccaa
    20161 attctgaatg tgtcgaatat gttcggcaaa ttcaaaaacc aatttgttgg ggtatcgcca
    20221 agcggtaagg caccggattc tgattccggc attccgaggt tcgaatcctc gtaccccagc
    20281 caatttattc aagacgctta ccttgtaagt gcacccagtt ggggtatcgc caagcggtaa
    20341 ggcaccggat tctgattccg gcattccgag gttcgaatcc tcgtacccca gccacattaa
    20401 aaaagctcgc ttcggcgagc tttttgcttt tctgcgtata ttcaatgccg aatgtgatgt
    20461 tgactcgtct tatccttcaa agtcggatgc gacgctgccg cgtcttatcc gacctacggt
    20521 tggcatgcat ccagcaatgt tgtgaggcta caaccctaac gcatatttca gcgccttacg
    20581 tttcaacacg ccagcacgct ccgccgccat caaaccaaga ttacgcacaa aacgcagcgg
    20641 tggcagatta ttgctgaatc cggcataaaa cagatccata ccactttgca tcatgaagtt
    20701 atccgccata cgccgcatct ggtaacgctt gagcacagga taactggccc acgcttcgcc
    20761 gtagctgcgg gcgttcacca gaacgtcaat cagggcatcg acatcacgat aaccaagatt
    20821 cactccctgt cccgccagcg gatggatggt atgcgcggca tcgcccacca gagcaagccc
    20881 tggttgcaca tactgcaacg catggcgacg tatcagcgga aacgcaccag cggcaagcgg
    20941 tgtcacgtaa cctagacgcg acgggaaatg cttcgcgatt tccgcctgta gctgcgccat
    21001 attcatattc tgcaactggc gaatgcgcgc cggagagtca taccacacca gcgatgccca
    21061 gttatcaaac aacggcagaa acgcacgcgg tccgtccggg gtaaattgct gccaggtgct
    21121 gtcgccggga tcgttctcac actgtacgct aatcaacata cacgactgcg catactgcca
    21181 ggcatgaacg ccaattcccg ccatctgtcg cacctgcgaa tttgcgccgt cggcaccaat
    21241 caccagcttc gcgaggatcg tttcaccgcc cttcagttcc agctcctgaa gatcattatg
    21301 gcgatgcagt gcaatcagcg aggttggcac acgtaacgtt actttcggat gcgcttccag
    21361 cgcctgccac aacgcctgtt gcaggacagt gttttccacc atgtagccaa gcaacggtag
    21421 cttaagttca gcggcgtcaa acaccacatg cgccgtttcc cactcccacg tttccagtct
    21481 gcggtaagga tggcaacgca tagcctgtac tgcatcccag acccctaacc ctttaagcaa
    21541 tgataccgaa gccgcactaa tcgccgagat ccgcacgtcc ggctggctgt cagcgacaaa
    21601 cggtgcaggt tgggcatgtt cgataaccat taccgaaaat ccgtgctgag ccagtcccag
    21661 cgccagtgcg ccgccgacca ttcctccgcc gacaatggca atttccgttg gttgatttgt
    21721 catggtcaat catcctgtta gcaatatgct ttaagtttac aggattttta cccccaaggg
    21781 ctgataacgc tcatactggt cacaacggca gcaaagcatt acactatgcg ccctgcattc
    21841 ctggctacta tttcgcaaga gcaagtcgat gaccaaaaaa ctccatatta aaacctgggg
    21901 ctgtcagatg aacgagtacg attcatcgaa gatggccgat ctgctggatg ccacccacgg
    21961 ctatcaactg accgacgtgg cggaagaagc ggatgtgctg ctgctgaaca cctgctcaat
    22021 ccgcgagaag gctcaggaaa aagtcttcca tcagttgggt cgctggaaac tgttaaaaga
    22081 gaagaatcca gacctgatta tcggcgtcgg cggctgcgtg gcatcgcaag aaggcgagca
    22141 cattcgccag cgcgcccact atgtcgatat tatttttggg ccgcaaacgc tgcatcgtct
    22201 accggagatg atcaactccg tgcgcggcaa ccgcagcccg gttgtagata tcagcttccc
    22261 ggaaatcgag aagtttgacc gtctgccaga accgcgcgcc gaagggccga ccgcgtttgt
    22321 ctccatcatg gaaggctgca ataaatattg cacctactgc gtggtgcctt acacccgtgg
    22381 tgaagaggta agccgtccgt ccgacgatat tctgtttgag atcgcccagc ttgcggctca
    22441 gggcgtgcgt gaagtcaacc tgctcggtca gaatgtgaat gcctggcgtg gtgagaacta
    22501 cgacggcacc accggaacgt ttgccgatct gctgcgtctg gttgctgcga tcgacgggat
    22561 cgatcgtatt cgctttacca ccagccatcc gatcgaattc accgacgata tcatcgaagt
    22621 gtatcgcgac acgccggagc tggtgagctt cctgcacctg ccggtacaga gcggttccga
    22681 tcgcattctg aacctgatgg gccgtactca cacggcgctg gagtacaaag cgatcatccg
    22741 taaactgcgt gcggcgcgtc cggatattca gatcagttct gacttcatcg ttggcttccc
    22801 tggcgaaacc accgacgact tcgagaaaac gatgaagctg attgccgacg tcaatttcga
    22861 catgagctac agctttatct tctccgcacg tccgggtacg ccagccgccg atatggttga
    22921 tgatgttccg gaagaagaga agaagcagcg tctgtatatt ctgcaagagc gcattaatca
    22981 gcaggcgatg gcatggagcc gccggatgct cgacactacc caacgtattc tggtggaagg
    23041 gacttcacgt aagagcatca tggagctttc cggtcgcact gaaaataacc gcgtggttaa
    23101 cttcgagggc acaccggata tgatcggtaa attcgtcgat gtagaaatta ccgacgtcta
    23161 cccgaactct ctgcgcggta aagtggtgcg aactgaagat gaaatgggtc tgcgcgtggc
    23221 agaaacaccg gaatcggtga ttgcccgtac ccgcaaagaa aacgaccttg gcgtgggtta
    23281 ttatcagccg taattctcag gcccgccgtt ccggtgggcc ttgttttttc tctctttatc
    23341 cccatcttta tcgcaatgtt tgcgcctttt tcctgtggcg tgaataattt ccttataggc
    23401 cggtgaatgt tcagcttccg gcggcataca gataagagga acggtttgaa catagacact
    23461 cgcgaaatca ccttggagcc agcagacaac gcacgtctgt tgagcctgtg cggcccgttt
    23521 gatgacaaca tcaaacaact cgaacgccgt ctcggcatcg aaatcaatcg ccgtgataac
    23581 cactttaaac tgaccggccg tccgatttgc gtcaccgctg cggcagacat tctgcgtagc
    23641 ctgtatgtcg atacagcccc gatgcgcggt cagattcagg atatcgaacc ggaacagatc
    23701 caccttgcga ttaaagaagc gcgggtactg gagcaaagcg cggagagcgt gccggagtac
    23761 ggcaaagcgg tcaatatcaa aaccaaacgc ggcgtaatta agccgcgcac gccaaaccag
    23821 gcgcagtaca tcgccaatat tctcgaccat gacattacct tcggcgttgg cccggcgggt
    23881 acgggtaaaa cctacctggc agtggctgcg gcagttgatg ccctggagcg tcaggaaatt
    23941 cgccgtattc tgctgactcg tccggcggtc gaagccggtg agaaactggg cttcctgcct
    24001 ggcgatttaa gccagaaagt agacccgtat ctgcgcccgc tgtacgacgc gctgtttgaa
    24061 atgctgggct ttgagaaagt cgagaaactg attgagcgca acgttattga agtcgcaccg
    24121 ctggcctata tgcgtggtcg tacgctgaac gacgcgttta tcattcttga tgagagccag
    24181 aacaccacca tcgaacagat gaagatgttc ctgacccgta tcggttttaa ctcaaaagcg
    24241 gttatcaccg gcgacgtcac acagatcgac ctgccgcgta ataccaagtc tggtttgcgt
    24301 catgccatcg aagtgctggc tgatgtcgaa gagatcagct ttaacttctt ccacagcgaa
    24361 gacgtggttc gtcacccggt ggtggcgcgt attgttaacg cctatgaagc ctgggaagaa
    24421 gccgaacaaa aacgtaaagc ggcgctggca gcagaacgca agcgcgaaga acaggaacaa
    24481 aaatgagtca ggtgatcctc gatttacaac tggcatgtga agataattcc gggttaccgg
    24541 aagagagcca gtttcagaca tggctgaatg cggtgatccc gcagtttcag gaagaatcgg
    24601 aagtgactat tcgcgtggtc gataccgccg aaagccacag tctgaatctg acctatcgcg
    24661 gtaaggataa gccgaccaac gtgctctcct tcccgtttga agtaccgcct ggcatggaaa
    24721 tgtcgctact gggcgatctg gttatctgcc gtcaggtggt tgagaaggaa gctcaggagc
    24781 aaagcaaacc actggaggcg cactgggcgc atatggtggt gcacggcagt ctgcatttgt
    24841 taggttacga tcacatcgaa gatgacgaag cagaagaaat ggaagccatc gaaacagaga
    24901 ttatgcttgc tctgggctat gaggatccgt acattgccga gaaagaataa tttgccaggc
    24961 atttgactgg cagacgtccc cgccgttggt gattaacgac ggcgttttga ttaactaaca
    25021 tgagaaccca tacgacgcca tgagcgacga caattcacac agtagtgaca cgataagcaa
    25081 caagaaggga tttttctccc tgttactcag ccaacttttc cacggtgaac cgaaaaaccg
    25141 tgacgaactg ctggcgctga tccgtgattc cgggcagaac gaccttatcg acgaagatac
    25201 gcgcgatatg ctcgaagggg tgatggacat cgcagaccaa cgcgttcgcg acatcatgat
    25261 cccccgctcc cagatgatta ccctgaaacg caaccagacg ctggacgaat gccttgatgt
    25321 catcattgag tccgcccact cacgtttccc ggtgattagc gaagacaaag atcacattga
    25381 agggattctg atggcgaaag acttgctgcc gtttatgcgc agcgatgctg aagccttcag
    25441 catggacaaa gtgttacgtc aggcggttgt cgttcctgaa agtaagcgcg tagaccggat
    25501 gctgaaagag tttcgctctc agcgttacca catggcgatc gtcattgacg aattcggtgg
    25561 ggtttccggt ctggtgacca ttgaagacat cctggaactg attgttggtg agattgaaga
    25621 cgagtatgac gaagaagatg atatcgactt ccgtcagctg agtcgtcata cctggactgt
    25681 gcgcgcactg gcttctattg aagacttcaa cgaagcgttc ggcacccact ttagcgatga
    25741 agaggttgac actatcggtg gtctggtgat gcaggcattt gggcatcttc cggcgcgtgg
    25801 cgaaaccatc gacatcgacg gttaccagtt caaagtggcg atggccgaca gtcggcgtat
    25861 tattcaggtt catgtcaaaa tcccggatga ctcaccccag ccgaagctgg atgaataaaa
    25921 ccgaaactgg atagataact acatggcttt tgcctcatta attgaacgcc agcgcattcg
    25981 cctgctgctg gcgttattat tcggtgcctg cggaacgctg gccttctctc cttacgacgt
    26041 ctggcctgcg gcgattattt cgctgatggg gcttcaggcg ttgaccttta accgccgtcc
    26101 actccagtct gccgctattg gcttttgctg gggatttggc ctctttggca gcggtattaa
    26161 ctgggtctat gtcagcatcg cgacctttgg cggaatgcct ggcccggtta acatcttcct
    26221 ggtggtactg ctggcggcgt atttgtcgct gtataccgga ctgtttgctg gcgtgctgtc
    26281 gcgtctgtgg ccgaaaacca cctggctgcg cgtggcgatt gccgcccctg ctctctggca
    26341 agtgaccgag tttctgcgcg gttgggtgct gactggcttc ccgtggttac agttcggcta
    26401 tagccagatt gatggcccgt taaaagggct ggcaccgata atgggcgtgg aagccattaa
    26461 cttcctgctg atgatggtta gtggcctgct ggcactggcg ttggtcaaac gcaactggcg
    26521 tccgctggtg gtgaccgtcg tgctgtttgc ccttcccttc ccgctgcgtt acatccagtg
    26581 gtttaccccg caaccggaga aaaccattca ggtttcgatg gttcagggcg atattccgca
    26641 atcgctgaaa tgggacgaag gccagcttct taatacgctg aagatttact acaacgcaac
    26701 ggcaccgctg atgggcaaat catcgctgat tatctggccg gagtcggcga taaccgatct
    26761 ggaaattaat cagcaaccgt tccttaaagc tctggacggt gagttgcgcg ataaaggtag
    26821 ctcgctggtc accgggattg tcgacgcgcg tctcaataaa cagaaccgct acgataccta
    26881 caacaccatc atcacactgg gtaaaggcgc accgtacagc tatgaatcag ccgatcgcta
    26941 taacaaaaac catctggtgc cgtttggcga gtttgtccca ctggagtcga tactgcgtcc
    27001 gttagcaccg ttctttgatt tgccgatgtc gtcattcagc cgcggaccat atattcagcc
    27061 accgctgtcg ttgaatagca ttcaacttac tgcggccatt tgctacgaaa tcattctcgg
    27121 cgagcaagtg cgcgataact tccgcccgga taccgactat ctgctgacta tctccaacga
    27181 tgcgtggttt ggtaagtcta ttggtccatg gcagcacttc cagatggcgc gaatgcgtgc
    27241 gctggagctg gcgcgcccac tgttgcgcag caccaacaac ggcattacgg cggtgattgg
    27301 cccgcagggt gagattcagg cgatgatccc gcagttcacc cgcgaggtgt tgaccactaa
    27361 cgtgacacca accaccggac tcacaccgta cgcacgaacc ggcaactggc cgctgtgggt
    27421 gctgacggcg ctgtttggtt ttgccgctgt actgatgagt ctgcgtcagc gacgtaaacg
    27481 ttgatgtgaa tgatgcgctg gcctgcaaaa ttgcatcaat tttgcaggcc agataaaccg
    27541 ttcacaccgc atccggtatc aacaaaacat actttctcat tgagttgtgg ggcatgatta
    27601 tcatgccctt ttttcttacg gttcagggat acaagccaca tcgccatatt ggggatgccg
    27661 aataattgcc tgcggatcaa gcgagcgcgt cctgtccatt tggattaacc tgttcttcgc
    27721 cataacaaat atctttctac catccgctga aaaacgaaga ttattaacgt agctggcggg
    27781 ataaacgcca ataatctgcc cggcattaat attgagcagg atgacataac cttgcaggcc
    27841 atatgacacc aacgctaaat actgaccgtc cgggctaata ccctcaacgg aataaacgct
    27901 cctgctacac cattgcgtca taatgctggc ttgttgatcc tcagctctta tacgtgaaat
    27961 cccctggcta tcggcaaaaa taacctcacc attttttggt cggaatccca tcgcagaaac
    28021 gacaccacac gcatcacctg cgggaaagtc ttctttctga tgcgtgatgg tattgatccg
    28081 cacgatgtta cctctgcctg ctcccggaac cagtaatgtt ttttcatccg gggaaagtgc
    28141 cataacccag gcgccaggtt taccgctcat ccccgtcccg tcgtatttct ccagcgggat
    28201 atccgccaca tgctgataag ccaccagatc aatcgctgac acactattgc tggtgcgatt
    28261 catcacccag gcttttgtgc catcacgcga gaggataaag ctgtcaggat gtgcgccggt
    28321 ggtaatcgac tgaataatct cccccgtcgc cacgttaatg accgataacg cattcgcttc
    28381 aaatgaagtc gcataaatca acttttcatc caggcttaat gccagcgatc tgacgccctg
    28441 atctttacag ccaccacaat gtttgactga cttttctttc gctatgggcg ctgtaaaggt
    28501 atgcaggatt ttcttttgtc cgaggtcgaa aacaacaatg ctgttcgtgt tttcaattgc
    28561 ggcatacaac gtattaccat cccgactgat cgccatatca tggatatcgg caatctcagc
    28621 gagtaaatca ccactggcct gctcacgact taccggagcg gataatgttt ccctgagggc
    28681 gttgcgctcc tccactaact gctgcttttt ttgtcgtcgc tcttgttcaa tctcctgcgg
    28741 ataaaacgct tcacgcaggc tttgcctgac ctgtaaaact gattcatcaa tctgctgctg
    28801 cacaaaccag atagtcccgg tcagaagaag agtgaccgca acaataatcc cgccttccgt
    28861 aacgagccga ttttgcccaa ctaaacgcgc actcagccaa cccaacgcgc cccccgcaat
    28921 caggccgcca atgtggcaag cgttatctat tcctgtttgc cgggtaccat acaacagtgt
    28981 cagcgccacc atacccaaca gaggaaatac tcgccgctgg tttttatggt gtgtgcccgc
    29041 acctgaagca agctgtgttg ctatcgcagc gcccgctatt cccataattg ccccggatgc
    29101 cccaacgctg atgactatct gctcactttc gcgtaacgca taacaggcgc tgatcagccc
    29161 accaaagatg ccggagaata accagataat caggagtcgc tatttcccca gtatccgttc
    29221 cgctactgtc cccaccgcca ggagtgcaaa catattcatc agcaaatggg aaaaactact
    29281 gtgcagaaaa acactgctca ataagcgcca ggattcatcc gacaacgtga gagaagcaac
    29341 gttagcgccg agatggatga gtgcggattg atcgacttct tcgtaactca tccattgtgc
    29401 gaggtaacaa aatatcccaa cacagagcgc aaaaaggatc agggttattg cgggtaattg
    29461 aacatttaac ggcttaaccg aagatgcaga cataaggtga ttcttccttg aaaagactag
    29521 taaaaagcat tttcccgtgt gtagttatca tcccggcaaa cgtcccacac cgaaaagtta
    29581 acactgtccg aaatatgact ttttcaaact gaaattacaa tcgtaattaa ttcaccccta
    29641 agtatttttt ggcatgtaag ttgctttatt tactgtgtaa gcaatgaaat cagccgcaac
    29701 gcaaacgggt tgcaccacca caaatcagcg gtagcaccaa aaaggtgcgg tttaagaatt
    29761 ttgcttcgga atggtgcggc gcacctcgca aaaataaaca ataaaacagc aatatcttta
    29821 catttatcca aactaaatgt tacctaacaa acacaacact gcacaataaa gtcgcagatg
    29881 ataacaacac aaacactcac aacgggtatc catgcgtttt taacgcagaa gataaaggag
    29941 ttggatatgc aattacgtaa acctgccaca gcaatcctgg ccctggcgct ttccgcagga
    30001 ctggcacagg cagatgacgc cgccccggca gcaggcagca cgctggacaa aatcgccaaa
    30061 aacggcgtga ttgtcgtcgg tcaccgtgaa tcttcagtac ctttctctta ttacgataat
    30121 cagcaaaaag tggtgggcta ctcgcaggat tactccaacg ccattgttga agcagtgaaa
    30181 aagaaactca acaaaccaga cttacaggtg aaattgattc cgatcacctc acaaaaccgt
    30241 attccactgc tgcaaaacgg cactttcgat tttgaatgcg gttctaccac caacaatgcc
    30301 gaacgccaaa aacaggcggc tttctctgac actattttcg tggtcggtac gcgcctgctg
    30361 accaaaaagg gcggcgatat caaagatttt gccgacctga aaggcaaagc cgtagtcgtc
    30421 acttccggca ccacctctga agttttgctc aacaaactga atgaagagca aaaaatgaat
    30481 atgcgaatta tcagcgccaa agatcacggt gactctttcc gtaccctgga aagcggtcgt
    30541 gccgttgcct ttatgatgga tgatgctctg ctggctggtg aacgtgcgaa agcgaagaaa
    30601 ccagacaact gggaaatcgt cggcaagccg cagtctcagg aagcctacgg ttgcatgttg
    30661 cgtaaagacg atccgcagtt caaaaagctg atggatgaca ccatcgctca ggtgcagacc
    30721 tccggtgaag cggaaaaatg gtttgataag tggttcaaaa atccaattcc gccgaaaaac
    30781 ctgaacatga atttcgaact gtcagacgaa atgaaagcac tgttcaaaga accgaatgac
    30841 aaggcactga actaattaca agaaccaggg gcggaaattc cagccctctc gattgttacg
    30901 tagcacggac agactatacg cctggtggtc gttccccatc gggcctgaaa accgcaatac
    30961 gctgggtaac aatcttcgag ggtagcagtt aacgctgcta ccctcttttt tctggagtag
    31021 atttatgtct atagactgga actggggtat ttttttacaa caagccccgt tcggcaacac
    31081 cacctatctt ggttggatct ggagtggctt tcaggtgacg atcgctttgt cgatctgcgc
    31141 ctggattatc gctttcctcg tcggttcatt tttcggcatt ttacgtaccg ttccaaaccg
    31201 ttttctctcc ggtctgggta cgttgtatgt cgaactgttc cgcaacgtgc cgttgattgt
    31261 gcaattcttt acctggtacc tggtgatccc ggagctgctg ccagagaaaa tcggcatgtg
    31321 gtttaaggcc gagctggatc ccaatattca gtttttcctt tcatccatgc tttgcctggg
    31381 gctgtttacc gccgcccgtg tttgcgaaca ggtacgcgcc gctattcagt cgctgccgcg
    31441 tgggcagaag aatgccgcgc tggcgatggg tctgacgcta ccgcaggctt accgctacgt
    31501 actgctgcct aatgcttatc gcgttatcgt cccgccgatg acctcagaga tgatgaacct
    31561 ggtgaaaaac tccgccatcg cctcgactat cggtctggtg gatatggcgg cgcaggcggg
    31621 taaattgctc gattactcgg cccacgcctg ggagtcgttt accgccatta cgctggcgta
    31681 tgtattgatt aacgctttta tcatgctggt gatgacgctg gttgaacgta aagttcgcct
    31741 gtccggcaac atggggggca aataatgtac gagtttgact ggagttccat tgttccttcc
    31801 ctgccatatc tgctcgacgg gctggtgatc acgctgaaaa tcaccgtcac ggcagtggtg
    31861 attggtattt tgtggggcac catgctggcg gtgatgcgct tgtccagctt tgcgcccgta
    31921 gcgtggtttg ccaaagccta cgttaacgta ttccgctcga ttcctttagt tatggttttg
    31981 ctgtggtttt acctgatcgt accgggtttt ctgcaaaacg tgctgggatt atcaccaaaa
    32041 aatgatatcc gtttaatttc ggcgatggtg gcgttttcga tgtttgaagc ggcgtactat
    32101 tcagagatta tccgtgctgg tattcaaagt atttctcgtg gtcagtcgag cgccgcgctg
    32161 gcgttgggaa tgactcactg gcagtcgatg aaactgatta ttctgccgca ggcgttccgc
    32221 gcgatggtgc cgctgttgct cactcagggc atcgtactgt ttcaggatac ctcactggtg
    32281 tatgtgttat ctctggccga tttcttccgt accgcctcaa ccattggtga gcgtgatggt
    32341 acgcaggttg agatgatcct gtttgccgga tttgtttatt tcgttattag ccttagcgcg
    32401 tcgttgttgg tcagctactt gaaaagaagg acagcataat gattaccctg aaaaatgttt
    32461 caaaatggta tggtcacttt caggtgctga ccgactgctc aaccgaagtg aaaaaaggcg
    32521 aagttgtggt ggtttgcggt ccgtctggtt ccggcaaatc aacgctgatt aaaaccgtca
    32581 acggcctcga accggtgcag caaggtgaaa tcaccgtcga tggcatcgtg gttaacgaca
    32641 agaaaaccga tctggcgaaa ctgcgttccc gcgtcgggat ggtgtttcag catttcgagc
    32701 tgttccccca tctgtcgatt atcgaaaacc tgaccctggc gcaggtgaaa gtgcttaaac
    32761 gcaataaagc gccggctcgt gaaaaagcat tgaaattgct ggagcgcgtc gggctttccg
    32821 cacatgccaa taagtttccg gcgcagcttt ccggcggcca gcagcagcgt gtggcgatcg
    32881 ctcgcgcatt gtgcatggat cctattgcca tgctgttcga cgaaccaacg tcggcgctgg
    32941 atccggagat gatcaacgaa gtgctggacg tgatggtcga actggcgaac gaaggaatga
    33001 cgatgatggt ggtgacccac gaaatgggct ttgcccgtaa agtggcgaat cgggtgatct
    33061 ttatggacga gggtaaaatt gtcgaagact cgccgaaaga cgctttcttc gatgatccga
    33121 aatcggaccg cgcaaaagac ttcctcgcaa aaatcctgca ttaatcactc tggcgcgcat
    33181 actcaattgc gcgccatctc acgctttctc gtttcgtccc tcgtatctgt tacatcccgg
    33241 cgttaccctt gtcgcaaaga aacacacaac aaggagcaac aatggcactg ccaattctgt
    33301 tagattgcga cccaggtcat gacgacgcta tcgcaatcgt tctcgccctc gcctcaccag
    33361 agcttgatgt caaagcaatt acgtcctccg ccggaaacca gacaccagaa aaaaccttac
    33421 gcaatgttct gcgtatgctg accttgctta atcgcaccga tattccggta gcaagcggtg
    33481 cggtaaaacc gttaatgcgt aatttgatta ttgcggacaa cgtgcacggc gaaagcggcc
    33541 tggacggccc ggcattgccg gaaccggcat ttgcaccgca aaactgcacg gcagtggagc
    33601 tgatggcgaa aacgctgcgt gaaagtgagg aacctgtcac cattgtttct accggaccgc
    33661 aaactaacgt tgccttgctg ctcaatagcc acccggaact gcatagcaaa attgcccgta
    33721 tcgtgattat gggcggcgca atggggcttg gtaactggac gcctgcggct gaatttaata
    33781 tttacgttga cccggaagcg gcagaaattg tcttccagtc aggaattccg gtggtgatgg
    33841 ccggtctgga tgttactcac aaagcacaaa tccacgttga agacactgag cgtttccgcg
    33901 cgattggcaa ccctgtttca accattgttg ccgaactgct ggatttcttc ctcgaatatc
    33961 acaaagacga aaaatggggc tttgtcggcg caccactgca tgacccatgc accatcgcct
    34021 ggctgttgaa accggagttg tttaccactg ttgagcgctg ggttggcgtt gaaacacaag
    34081 ggaaatatac ccagggtatg acggttgttg attattatta tctgactggc aataaaccga
    34141 atgccaccgt aatggtcgat gttgatcgtc agggctttgt tgatttactg gctgatcgtc
    34201 tgaaatttta cgcttaatca ctcgccggat gacatcacgc catccggcac cactcatttc
    34261 attacggctc gaacggacgt ctctggaact gatcatgacc acatttaggg catagcgtca
    34321 gcacttccgg tgtgtagatc gggagatgga agtgacattt ctcgcagacc agatttccca
    34381 gccctaccac ttctccgctg tgataaaccc catgatgatt gaggtcctgg aaaacttcgc
    34441 gccattcaag ctgcgtttta tcggtgatgt ctgccagctc ctgccacaag ctttctttaa
    34501 tcacccgcat aaagacgctg tcagactctt ctttcaggct ctcttcatag ctcatggcga
    34561 actcttccag gtcacgtctg acagctcgcg tcagctcatc gacctcggtt cgcgttaact
    34621 cccctgtttt tattacgcgc tcgcgcgcct gttccaccag tgcgtcgata tcacgttcgc
    34681 cattgcgcag gcgttcgctc agtgacgcga ccagttcacg gtaatgttga gcaaccttgt
    34741 tcatcgtttc atctcccggg tcgttaactg actataataa tagacgttct ttgttccttg
    34801 ctttgctaat acgccaacaa actctgtaaa ttaatgtatg aataatccgg gcatcatttc
    34861 aacgtcgagt gcgcgaaagg ctgttttgac gcgggcgttt gggctatgct atgcggatct
    34921 gaaaaaccac atcaacgcta catttgtagc cgtattgaaa acaggaccac tggctgccat
    34981 gcaagagcaa taccgcccgg aagagataga atccaaagta cagcttcact gggatgagaa
    35041 gcgcacattt gaagtaaccg aagacgagag caaagagaag tattactgcc tgtctatgct
    35101 tccctatcct tctggtcgac tacacatggg ccacgttcgc aactacacca tcggtgacgt
    35161 gatcgcccgc taccagcgca tgctgggcaa aaacgttctt cagccaatcg gctgggacgc
    35221 atttggtctg cctgcggaag gcgcggcggt gaaaaacaac accgcgccag caccgtggac
    35281 gtacgacaac atcgcgtata tgaaaaacca gctcaaaatg ctgggctttg gttatgactg
    35341 gagccgcgag ctggcgacct gtacgccgga atactaccgt tgggaacaga aattcttcac
    35401 cgagctgtat aaaaaaggcc tggtatataa gaagacttca gcggtcaact ggtgcccgaa
    35461 cgaccagacc gtactggcga acgaacaagt tatcgacggc tgctgctggc gctgcgatac
    35521 caaagttgag cgtaaagaga tcccgcagtg gtttatcaaa atcactgctt acgctgacga
    35581 gctgctcaac gatctggata aactggatca ctggccagac accgttaaaa caatgcagcg
    35641 taactggatc ggtcgttccg aaggcgtgga aatcaccttc aacgttaacg actatgacaa
    35701 cacgctgacc gtttacacta cccgcccgga cacctttatg ggttgtacct acctggcggt
    35761 ggctgcgggt catccgctgg cacagaaagc ggcggaaaat aatcctgaac tggccgcctt
    35821 tattgacgaa tgccgtaaca ccaaagttgc cgaagctgaa atggcgacga tggagaaaaa
    35881 aggcgtcgat actggcttta aagcggttca cccattaacg ggcgaagaaa ttcccgtttg
    35941 ggcagcaaac ttcgtattga tggaatacgg cacgggcgca gttatggcag ttccgggtca
    36001 cgaccagcgc gactacgagt ttgcctctaa atacggcctg aacatcaaac cggttatcct
    36061 ggcagctgac ggctctgagc cagatctttc tcagcaagcc ctgactgaaa aaggcgtgct
    36121 gttcaactct ggcgagttta atggtcttga ccatgaagcg gccttcaacg ccatcgccga
    36181 taaactgact gcgatgggcg ttggcgagcg taaagtgaac taccgcctgc gcgactgggg
    36241 tgtttctcgt cagcgttact ggggcgcgcc gattccgatg gtgacgctgg aagacggtac
    36301 cgtaatgccg accccggacg accagctgcc ggtgatcctg ccggaagatg tggtgatgga
    36361 cggcattacc agcccgatta aagcagatcc ggaatgggca aaaactaccg ttaacggtat
    36421 gccagcactg cgtgaaaccg acactttcga cacctttatg gagtcctcct ggtactatgc
    36481 gcgctacact tgcccggagt acaaagaagg tatgctggat tccaaagcgg ctaactactg
    36541 gctgccggtg gatatttaca ttggtggtat cgaacacgcc attatgcacc tgctctactt
    36601 ccgcttcttc cacaaactga tgcgtgatgc aggcatggtg aactctgacg aaccagcgaa
    36661 acagttgctg tgtcagggta tggtgctggc agatgccttc tactatgttg gtgaaaacgg
    36721 cgaacgtaac tgggtttccc cggttgatgc tatcgttgaa cgtgacgaga aaggccgtat
    36781 cgtgaaagcg aaagatgcgg caggccatga actggtttat accggcatga gcaaaatgtc
    36841 caagtcgaag aacaacggta tcgacccgca ggtgatggtt gaacgttacg gcgcggacac
    36901 cgttcgtctg tttatgatgt ttgcttctcc ggctgatatg actctcgaat ggcaggagtc
    36961 cggcgtggaa ggggctaacc gcttcctgaa acgtgtctgg aaactggtgt acgagcacac
    37021 agcaaaaggt gatgttgcgg cactgaacgt cgatgcgctg actgaagatc agaaagcgct
    37081 gcgtcgcgat gtgcataaaa ctatcgctaa agtaaccgat gatatcggcc gtcgtcagac
    37141 cttcaacacc gcaattgcgg cgattatgga gctgatgaac aaactggcga aagcaccgac
    37201 cgatggcgag caggatcgcg ccctgatgca ggaagcgctg ctggcggtag tccgtatgct
    37261 taacccgttc accccgcaca tctgcttcac gctgtggcag gaactgaaag gcgaaggcga
    37321 tatcgacaac gcgccgtggc cggttgctga cgaaaaagcg atggtggaag actccacgct
    37381 ggtcgtggtg caggttaacg gtaaagtccg tgcaaaaatc accgttccgg tggacgcaac
    37441 ggaagaacag gttcgcgaac gtgctggcca ggaacatctg gtagcaaaat atcttgatgg
    37501 cgttactgta cgtaaagtga tttacgtacc aggtaaactc ctcaatctgg tcgttggcta
    37561 agcgcgggag gaagcgtgcg atatctggca acattgttgt tatctctggc ggtgttaatc
    37621 accgccgggt gtggctggca tctgcgtgac accacgcagg ttccttccac tatgaaagtc
    37681 atgatcctgg actcaggcga tccgaacggg ccattaagcc gtgcggtgcg taaccagtta
    37741 cgtctgaatg gtgtcgagtt gcttgataaa gaaaccacgc gtaaggacgt tccatccttg
    37801 cgtttgggta aagtgagcat cgcgaaagat accgcatcgg tattccgtaa cggtcaaaca
    37861 gcagagtatc agatgatcat gacggtgaat gcgaccgtgt tgatccccgg ccgtgacatc
    37921 tacccgatta gcgccaaagt cttccgttcg ttcttcgata acccgcaaat ggcgttagcg
    37981 aaagataacg aacaagacat gatcgtaaaa gagatgtacg accgtgctgc cgaacagctg
    38041 attcgtaagc tgccaagcat ccgtgctgcg gatattcgtt ccgacgaaga acagacgtcg
    38101 accacaacgg atactccggc aacgcctgca cgcgtctcca ccacgctggg taactgatga
    38161 ttcggttgta cccggaacaa ctccgcgcgc agctcaatga agggctgcgc gcggcgtatc
    38221 ttttacttgg taacgatcct ctgttattgc aggaaagcca ggacgctgtt cgtcaggtcg
    38281 ctgcggctca agggttcgaa gaacaccaca ctttttccat tgatcccaac accgactgga
    38341 atgcgatttt ttcgttatgc caggccatga gcctgtttgc cagtcgacaa acgctattgc
    38401 tgttgttacc cgaaaacgga ccgaatgcgg cgatcaatga acaacttctc acactcaccg
    38461 gacttctgca tgacgatcta ctgttgatcg tccgcggtaa taaattaagc aaagcgcaag
    38521 aaaatgccgc ctggtttact gctcttgcga atcgcagcgt gcaggtgacc tgtcagacac
    38581 cggagcaggc tcagctgccc cgctgggttg ctgcgcgcgc aaaacagctc aacttagaac
    38641 tggatgacgc tgcaaatcag gtgctctgct actgttatga aggtaacctg ctggcgctgg
    38701 cccaggcact ggagcgttta tcgctactct ggccggacgg caaactgaca ttaccgcgcg
    38761 ttgaacaggc ggtgaatgat gccgcgcatt tcaccccttt tcattgggtt gatgctttgt
    38821 tgatgggaaa aagtaagcgc gcgttgcata ttcttcagca actgcgtctg gaaggcagcg
    38881 aaccggttat tttgttgcgc acattacaac gtgaactgtt gttactggtt aacctgaaac
    38941 gccagtctgc ccatacgcca ctgcgtgcgt tgtttgataa gcatcgggta tggcagaacc
    39001 gccggggcat gatgggcgag gcgttaaatc gcttaagcca gcagcagtta cgtcaggccg
    39061 tgcaactcct gacacgaacg gaactcaccc tcaaacaaga ttacggtcag tcagtgtggg
    39121 cagagctgga agggttatct cttctgttgt gccataaacc cctggcggac gtatttatcg
    39181 acggttgata tgaaatcttt acaggctctg tttggcggca cctttgatcc ggtgcactat
    39241 ggccatctta aacccgtgga aacgctggcg aatttgattg gtctgacgcg ggtcacaatc
    39301 atccctaata atgttcctcc gcatcgtccc cagccggaag cgaacagcgt gcagcgtaaa
    39361 cacatgcttg aactggcgat tgccgacaag ccattattta ctcttgatga acgcgagcta
    39421 aagcgcaatg ccccctctta cactgcgcaa acactgaaag agtggcggca ggaacaaggg
    39481 ccagacgtgc cgctggcgtt tattattggt caggattcat tgctgacctt tccgacctgg
    39541 tacgaatacg aaacgatact cgacaatgca catttgatcg tctgtcggcg tccaggttac
    39601 ccacttgaaa tggcacaacc gcaataccag caatggctgg aagatcattt gacacataac
    39661 ccggaagatc ttcaccttca gcctgccggt aaaatttatc tggctgaaac gccgtggttt
    39721 aacatctcgg cgaccatcat ccgcgaacgt ttgcaaaatg gtgaatcgtg tgaggattta
    39781 ttaccggaac cggtactgac ttacattaac caacaaggct tgtatcgctg atacctgtcg
    39841 ttacggaggg catgatgcga ctgtggttaa ttcgtcatgg tgaaacgcaa gcgaatgtcg
    39901 atggtctgta cagcggtcat gcgcccacac ccctgaccgc gcgcggtatt gagcaagcgc
    39961 aaaatctgca tacgctgcta catgatgttt cctttgacct ggttttatgc agtgaactgg
    40021 aacgggcaca gcataccgcg cgactggttc ttagtgaccg ccagcacccc gttcatatca
    40081 tccctgaact caatgaaatg ttttttggcg actgggagat gcgacatcat cgtgacctca
    40141 tgcaagaaga tgccgaaaac tatagcgcgt ggtgcaatga ctggcagcat gctatcccca
    40201 cgaacggtga aggatttcag gcattttcgc aacgtgtgga acgatttatc gcaaggctta
    40261 gtgaatatca gcactatcag aatattttag tcgtcagcca tcagggcgta ctgagtctgt
    40321 taattactcg tttaattggt atgcctgccg agtctatgtg gcatttccgc gttgaccagg
    40381 ggtgttggag cgccattgat atcaaccaaa aattcgcgac gctacgcgtc ctcaatagcc
    40441 gtgccatcgg ggtcgagaat gcatgacttt tctgtttttt tacgggtaag ccgcaacgac
    40501 cattgacagc cccgggcagg ctgatattct ccgcagccag actttttccg ccagacacga
    40561 ctttgtagaa attgttttac aaaaatggcg atgcaatctg cggcgcgggg tgggatgata
    40621 gcccactttc gaaagccgat tcggcgacaa ttgtcccgaa atcgcctctg gttcaggtat
    40681 actgacagac catttttatc tatttgattc acccaggggg aaaacttgca gggtaaagca
    40741 ctccaggatt ttgttatcga caaaattgat gacctcaaag gtcaggacat catcgcctta
    40801 gacgttcagg gcaaatccag catcaccgac tgcatgatca tctgtacggg tacgtccagc
    40861 cgccatgtta tgtccattgc tgatcacgtt gtgcaggagt ctcgcgcagc gggcctgtta
    40921 ccgctcggcg tagaaggtga aaacagcgcc gactggattg tcgtggattt gggcgatgtg
    40981 attgtccatg tcatgcagga agagagccgt cgcctgtatg aactggaaaa actctggagt
    41041 taatgcgtga agctgcaact tgtcgccgtg ggaacgaaaa tgccggactg ggtacaaacc
    41101 ggttttaccg agtacctgcg tcgttttccg aaagatatac ccttcgagct gattgaaatt
    41161 ccggccggaa aacgcggcaa gaatgcggac atcaagcgca tactcgacaa agagggtgag
    41221 cagatgttgg cggccgcagg caaaaaccgc attgtcaccc tcgatattcc aggcaagccc
    41281 tgggatacgc cgcagttagc cgctgagctg gaacgctgga agctggatgg tcgcgacgtc
    41341 agtctactga ttggcgggcc tgaagggttg tcgcctgcct gtaaagcggc ggcggagcag
    41401 agctggtcgc tgtcggcact tacccttccc catccgctgg ttcgcgtgct ggtcgcagag
    41461 agtctgtacc gggcgtggag catcaccacc aaccatcctt atcaccgtga gtgataagtg
    41521 agctttgagt agaaaacgca gcggatgaaa ctacagaact cttttcgcga ctatacggca
    41581 gagtccgcgc tgtttgtgcg ccgggcgctg gtcgcctttt tggggatttt gctgctgacc
    41641 ggcgtgctta tcgccaacct gtataatctg caaattgttc gctttaccga ctaccagacc
    41701 cgctctaatg aaaaccgcat taagctggtg cctatcgcac ccagccgcgg cattatctac
    41761 gaccgtaacg gtatccctct ggctctcaac cgcactatct accagataga aatgatgccg
    41821 gagaaagtcg ataacgtgca gcaaacgctg gacgctttgc gcagcgtggt tgatctgacc
    41881 gatgatgata ttgctgcatt ccgaaaagag cgcgcacgtt cacaccgttt cacctctatt
    41941 ccggtgaaaa ctaacctgac cgaagtacaa gtagctcgct ttgccgtcaa ccagtaccgt
    42001 tttcctggcg tcgaagttaa aggctataaa cgtcgttact atccttacgg ttcagcgttg
    42061 acccacgtca tcggctatgt gtcgaaaatc aacgataaag acgtcgaacg cctgaataat
    42121 gacggcaaac tggccaacta tgcggcaacg catgatatcg gtaagctggg cattgagcgt
    42181 tactatgaag acgtgctgca cggtcagacc ggttatgaag aggttgaagt taacaaccgt
    42241 gggcgtgtta tccgccagtt aaaagaagta ccaccgcaag ccggacacga tatttacctg
    42301 acgctggatc tcaaactcca gcaatatatt gaaacgctgc tggcgggtag ccgcgcagcg
    42361 gtggtagtca ccgatccgcg tacaggtggg gtgctggcgc tggtttccac gcctagttat
    42421 gacccaaacc tatttgttga cggtatctcc agcaaagatt attccgcctt gctgaacgac
    42481 ccgaatacac cgctggtgaa ccgcgccaca cagggggttt atcctcccgc gtctacagtt
    42541 aaaccctatg tggcggtttc ggcattgagc gccggagtga tcacgcgcaa tacgacgctg
    42601 tttgaccctg gctggtggca actgccaggg tcggaaaaac gttatcgtga ctggaaaaaa
    42661 tggggccacg ggcgtctgaa tgtcacaaga tcgctggaag aatctgcgga taccttcttc
    42721 tatcaggtgg cctacgatat gggaatcgat cgcctctccg aatggatggg taaattcggt
    42781 tatggtcatt acaccggtat cgacctggcg gaagaacgtt ccggcaacat gcctacccgc
    42841 gaatggaaac agaaacgctt taaaaaaccg tggtatcagg gtgacaccat tccggttggt
    42901 atcggtcagg gttactggac agcgacccca atccagatga gtaaggcact gatgatcctg
    42961 attaatgacg gtatcgtgaa ggttccgcat ttgctgatga gcaccgccga agacggcaaa
    43021 caggtgccat gggtacaacc gcatgaaccg cccgtcggcg atattcattc cggttactgg
    43081 gagctggcaa aagacggtat gtacggtgtt gctaaccgcc ctaacggtac tgcgcataaa
    43141 tactttgcca gcgcaccgta caaaattgcg gcgaaatctg gtacagctca ggtcttcggt
    43201 ctgaaagcga acgaaaccta taatgcgcac aaaattgccg agcgtttacg tgaccacaaa
    43261 ctgatgaccg cctttgcgcc atacaacaat ccgcaagtcg ctgtcgccat gattctggag
    43321 aacggcggtg cgggtccggc ggttggtacg ctgatgcgcc agatcctcga ccacattatg
    43381 ctgggtgata acaacaccga tctgcctgcg gaaaatccag cggttgccgc agcggaggac
    43441 cattaatcat gacggataat ccgaataaaa aaacattctg ggataaagtc catctcgatc
    43501 ccacaatgct gctgatctta ctggcattgc tggtttacag cgccctggtt atctggagcg
    43561 ccagcggtca ggatattggc atgatggagc gtaaaatcgg ccagattgcg atgggtctgg
    43621 tcatcatggt ggtgatggcg caaattcctc cacgcgttta tgaaggctgg gccccctatc
    43681 tctatatcat ctgtattatt ttgctggtgg cggtagatgc tttcggtgcc atctctaaag
    43741 gcgctcaacg ctggctggac ctcggtattg ttcgttttca accgtcggaa attgccaaga
    43801 tagccgtacc gttgatggtt gcacgcttta tcaaccgcga cgtttgccca ccttcattga
    43861 agaatacagc tatcgcgctg gtgctgatat ttatgcccac gctgctggtg gctgcacagc
    43921 ctgacttggg aacatcaatc ctcgttgcgc tttccggcct gtttgtactg ttcctctctg
    43981 gccttagctg gcgtctgatt ggcgtcgcag tagtgctggt agcggcgttc attccgattc
    44041 tgtggttctt cctgatgcat gattaccagc gccagcgcgt aatgatgctc ctggacccgg
    44101 aatcagaccc actcggcgca ggctatcaca ttattcagtc taaaattgct attggctccg
    44161 gcggattacg cggcaaaggc tggctgcacg gcactcagtc acagcttgaa tttctccccg
    44221 aacgccatac cgactttatc ttcgcggtac tggcggaaga gctgggatta gtgggcattt
    44281 tgattctgct cgctctctac attctgctga tcatgcgcgg gctgtggata gccgccagag
    44341 cgcaaaccac ctttggtcgc gtcatggctg gcggcttaat gctgatatta ttcgtttatg
    44401 tcttcgtaaa tattggtatg gtaagcggta ttctgccggt tgtaggggtt ccgctcccac
    44461 tggtcagtta tggaggatcg gcgctaattg tgctgatggc tgggttcggg attgtaatgt
    44521 caatccacac ccacaggaaa atgttgtcga aaagcgtgta agaggtgcgc aatgcgtaag
    44581 cagtggctcg ggatctgcat cgcggcagga atgctcacgg catgtacaag cgatgatggt
    44641 cagcaacaga cggtaagtgt accgcagcct gcggtatgta acggccccat agttgaaatt
    44701 agcggggcag acccgcgttt cgaaccactg aacgcgacgg caaatcagga ttaccagcgc
    44761 gacggtaaaa gctacaaaat cgtgcaggat ccgtctcgat ttagccaggc gggactggcg
    44821 gcaatctatg atgccgaacc cggcagtaac ctgacggcct ctggcgaagc tttcgatccg
    44881 acacagttga cggcggccca tccaacgctt ccaatcccca gctacgccag aatcactaac
    44941 ctggctaacg ggcgaatgat cgtggtgcgc attaatgatc gcggccctta tggcaacgac
    45001 cgcgtgattt cgctttctcg cgcggcagct gaccgtctta acacgtcaaa caacaccaaa
    45061 gttcgtatcg atccgattat tgtcgcccag gatggttcgc tttctggtcc tggtatggct
    45121 tgtaccacag tcgccaaaca gacttacgcc ctgccagcac ctcccgattt aagcggtggc
    45181 gcgggaacaa gttcagtgcc tgtcccgcag ggtgacattc ttccggtcag taattcgacg
    45241 ctaaaaagcg aagatccgac cggcgcgccg gtaaccagca gcggtttcct cggcgcacca
    45301 acgaccttag cgcctggtgt actggaaggc agcgaaccga cgcctgcgcc acagcccgtt
    45361 gttacagctc cgtcgacaac gcctgcaacc tcacctgcaa tggtgacacc gcaagccgcc
    45421 tcgcaaagcg ccagcggcaa ctttatggtg caagtcgggg ccgttagcga tcaggctcgt
    45481 gcgcaacagt accaacagca actgggacag aagttcggcg tccccggtcg cgtaactcaa
    45541 aatggcgcgg tctggcggat ccagcttggc ccattcgcca gcaaagccga agccagtacc
    45601 ttgcagcaac gtttgcaaac cgaagcccaa ttacagtcat ttattaccac cgcgcagtag
    45661 cgtaaagcag gcatctgatg tgtcaatttc tgtaaatgac gttaacaaag tcacgcggaa
    45721 agtcagatgc ctgccggtag tgcatttgct atagtagggc acttttttta attccatcac
    45781 ggatgtcgta gttcagacca tgaataccat tttttccgct cgtatcatga agcgcctggc
    45841 gctcaccacg gctctttgca cagcctttat ctctgctgca catgccgatg acctgaatat
    45901 caaaaccatg atcccgggtg taccgcagat cgacgcggag tcctacatcc tgattgacta
    45961 taactccggc aaagtactcg ccgaacagaa cgctgatgtc cgccgcgatc ctgccagcct
    46021 gaccaaaatg atgaccagtt acgttatcgg ccaggcaatg aaagccggta aatttaaaga
    46081 aactgattta gtcactatcg gcaacgacgc atgggccacc ggtaacccgg tgtttaaagg
    46141 ttcttcgctg atgttcctca aaccgggcat gcaggttccg gtttctcagc tgatccgcgg
    46201 tattaacctg caatcgggta acgatgcttg tgtcgccatg gccgattttg ccgctggtag
    46261 ccaggacgct tttgttggct tgatgaacag ctacgttaac gccctgggcc tgaaaaatac
    46321 ccacttccag acggtacacg gtctggatgc tgatggtcag tacagctccg cgcgagatat
    46381 ggcgctgatc ggccaggcgt tgatccgtga cgtaccgaat gaatactcga tctataaaga
    46441 aaaagaattt acgtttaacg gtattcgcca gctaaaccgt aacggcctgt tatgggataa
    46501 cagcctgaat gtcgacggca tcaaaaccgg acacactgac aaagcaggtt acaaccttgt
    46561 tgcttctgcg actgaaggcc agatgcgctt gatctctgcg gtgatgggcg gacgtacttt
    46621 taaaggccgt gaagccgaaa gtaaaaaatt actaacctgg ggcttccgtt tcttcgaaac
    46681 cgttaaccca ctgaaagtag gtaaagagtt cgcctctgaa ccggtttggt ttggtgattc
    46741 tgatcgcgct tcgttagggg ttgataaaga cgtgtacctg accattccgc gtggtcgcat
    46801 gaaagatctg aaagccagct atgtgctgaa cagcagtgaa ttgcatgcgc cgctgcaaaa
    46861 gaatcaggtc gtcggaacta tcaacttcca gcttgatggc aaaacgatcg aacaacgccc
    46921 gctggttgtg ctgcaagaaa tcccggaagg taacttcttc ggcaaaatca ttgattacat
    46981 taaattaatg ttccatcact ggtttggtta aaaattgaac acttgaaagt gtaatttccg
    47041 tccccatata ctaagcatca gtaaaaaact cccgccttct ggcgggagtt gctatttaat
    47101 tacgttacgc cggagctgac atgaaaacca aacttaacga actgcttgaa ttccctactc
    47161 cttttactta caaagttatg gggcaggcgt tacctgagct ggttgatcag gtggttgaag
    47221 tggtacagcg ccatgcgcca ggtgactaca ccccaacggt aaaaccaagc agcaaaggca
    47281 actaccactc ggtatctatc actatcaacg ccactcatat cgagcaggtt gaaacactgt
    47341 atgaagaact gggcaaaatc gatattgtcc gcatggttct gtaactcgct tctccgttac
    47401 ccggctcctg gtcgggtaac tcccctctcc tcgctgtgat atacttttcc cccactttta
    47461 ctcattctcc acggagacgc cgttttgtat caggataaaa ttcttgtccg ccagctcggt
    47521 cttcagcctt acgagccaat ctcccaggct atgcatgaat tcaccgatac ccgcgatgat
    47581 agtacccttg atgaaatctg gctggtcgag cactatccgg tattcaccca agggcaggca
    47641 ggaaaggcgg agcatatttt aatgccgggt gatattccgt tgatccagag cgatcgcggt
    47701 gggcaggtga cttatcacgg gccggggcaa caggtgatgt atgtgttgct taacctgaaa
    47761 cgccgtaaac tcggtgtgcg tgaactggtg accttgcttg agcaaacagt ggtgaatacc
    47821 ctggctgaac tgggtataga agcgcatcct cgggctgacg cgcctggtgt ctatgtcggg
    47881 gaaaagaaaa tttgctcact gggtttacgt attcgacgcg gttgttcatt ccacggtctg
    47941 gcattaaacg tcaatatgga tctttcacca tttttacgta ttaatccttg tgggtatgca
    48001 ggaatggaaa tggctaaaat atcacaatgg aaacccgaag cgacgactaa taatattgca
    48061 ccacgtttac tggaaaatat tttagcgcta ctaaacaatc ctgacttcga atatattacc
    48121 gcttaattcc atacatcaat ggcccaattt acactgggtc attactcata atacctgcta
    48181 atgctttaca tctccttcgc gtttgtatat tttctttatc acttgcagaa atgcgatgct
    48241 acgccttgct ccgtagccca cctaattgat taactcattg aaataattga ccatggtgag
//
LOCUS       DAZFFE010000023        43485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFFE010000023 DAZFFE010000000
VERSION     DAZFFE010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.023"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..79)
                     /locus_tag="ACHJ69_001546"
     CDS             complement(<1..79)
                     /locus_tag="ACHJ69_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310734.3"
                     /note="induced by CusR in the presence of copper; YedW
                     induces the expression of the upstream gene yedV (encoding
                     a sensor kinase) as well as yedW; yedVW is one of four
                     copper regulons found in E. coli; part of the copper
                     homeostasis mechanism; confers resistance to copper and
                     several drugs when induced; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFO5014554.1"
                     /translation="MSFEDSEKTSRVTLQQHYNFVMNQAV"
     gene            101..514
                     /gene="hiuH"
                     /locus_tag="ACHJ69_001547"
     CDS             101..514
                     /gene="hiuH"
                     /locus_tag="ACHJ69_001547"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO5014555.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            623..1627
                     /gene="msrP"
                     /locus_tag="ACHJ69_001548"
     CDS             623..1627
                     /gene="msrP"
                     /locus_tag="ACHJ69_001548"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO5014556.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKALEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGSP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            1628..2263
                     /gene="msrQ"
                     /locus_tag="ACHJ69_001549"
     CDS             1628..2263
                     /gene="msrQ"
                     /locus_tag="ACHJ69_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO5014557.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWVINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            2521..3171
                     /gene="zinT"
                     /locus_tag="ACHJ69_001550"
     CDS             2521..3171
                     /gene="zinT"
                     /locus_tag="ACHJ69_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO5014558.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDTQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(3841..3930)
                     /locus_tag="ACHJ69_001551"
     tRNA            complement(3841..3930)
                     /locus_tag="ACHJ69_001551"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3894..3896),aa:Ser,seq:cga)
     gene            4024..4821
                     /gene="mtfA"
                     /locus_tag="ACHJ69_001552"
     CDS             4024..4821
                     /gene="mtfA"
                     /locus_tag="ACHJ69_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO5014559.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            4922..4997
                     /locus_tag="ACHJ69_001553"
     tRNA            4922..4997
                     /locus_tag="ACHJ69_001553"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4955..4957,aa:Asn,seq:gtt)
     gene            5159..6421
                     /locus_tag="ACHJ69_001554"
     CDS             5159..6421
                     /locus_tag="ACHJ69_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5014560.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLRVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAVRGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLQKGIEEKGLLEV
                     ASRTRQHLSNIIRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVHYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
     gene            complement(6615..7919)
                     /gene="ybtS"
                     /locus_tag="ACHJ69_001555"
     CDS             complement(6615..7919)
                     /gene="ybtS"
                     /locus_tag="ACHJ69_001555"
                     /EC_number="4.2.99.21"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     Irp9/YbtS"
                     /protein_id="HFO5014561.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVSREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLAENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(7947..9227)
                     /gene="ybtX"
                     /locus_tag="ACHJ69_001556"
     CDS             complement(7947..9227)
                     /gene="ybtX"
                     /locus_tag="ACHJ69_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HFO5014562.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFGREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFLMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     PHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(9220..11022)
                     /gene="ybtQ"
                     /locus_tag="ACHJ69_001557"
     CDS             complement(9220..11022)
                     /gene="ybtQ"
                     /locus_tag="ACHJ69_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HFO5014563.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPPPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIARPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVHNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGGSASGEWVHE"
     gene            complement(11009..12721)
                     /gene="ybtP"
                     /locus_tag="ACHJ69_001558"
     CDS             complement(11009..12721)
                     /gene="ybtP"
                     /locus_tag="ACHJ69_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HFO5014564.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSEN
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFADTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            12978..13937
                     /gene="ybtA"
                     /locus_tag="ACHJ69_001559"
     CDS             12978..13937
                     /gene="ybtA"
                     /locus_tag="ACHJ69_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HFO5014565.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHGGFSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            14128..20235
                     /gene="irp2"
                     /locus_tag="ACHJ69_001560"
     CDS             14128..20235
                     /gene="irp2"
                     /locus_tag="ACHJ69_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HFO5014566.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDDARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLHPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     VLAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAHWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLAGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLATHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLM
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     IVRDGQQQVLEQTPPWVIPAHTLHTPEEALRVREKLAHQVLNPEVWPVFDLQVGYADG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPEFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTKRPRPVRRRQRRI"
     gene            20323..29814
                     /gene="irp1"
                     /locus_tag="ACHJ69_001561"
     CDS             20323..29814
                     /gene="irp1"
                     /locus_tag="ACHJ69_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HFO5014567.1"
                     /translation="MDNLRFSSAPTADSIDASIAQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGTVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWAGEKSGALVYSGHGASGKQVWLFTGQGSHWRTMGQ
                     TMYHHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGARVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLEALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELAGYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     PLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPNAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPEQFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDHPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTPWETLHQGLSAVSLPQRWLAARGDTLWLPSLSPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLAPRVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALACGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAAS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHFIGYGGVACHVLFEWDKRHDEFDLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRQTSAWHDAWDYWQEKLPQLPLAPELPVVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPIVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIARHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAIVQALSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQNQWSRALAQYLETCI
                     ARHDALFSGQCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRNTRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFT
                     AHPEAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGF
                     IEGLSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPG
                     VNRPDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDAPTEPAKPTPVAGGNPA
                     LEKQVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFSHST
                     LSDFCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLASALNRRVFGLQ
                     AKSPQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGKQVRM
                     VLIDPVCRQDFCCENRAALLRLLAEGQTPLALPEHFDQQTPDSQLADFISLAKTAGMV
                     SQNLTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPERWTPAETEWQGR
                     INNADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            29811..30911
                     /gene="ybtU"
                     /locus_tag="ACHJ69_001562"
     CDS             29811..30911
                     /gene="ybtU"
                     /locus_tag="ACHJ69_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HFO5014568.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPPVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLFVADHQENAHSLWRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSHLAGEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            30908..31711
                     /gene="ybtT"
                     /locus_tag="ACHJ69_001563"
     CDS             30908..31711
                     /gene="ybtT"
                     /locus_tag="ACHJ69_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HFO5014569.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDHEASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            31715..33292
                     /gene="ybtE"
                     /locus_tag="ACHJ69_001564"
     CDS             31715..33292
                     /gene="ybtE"
                     /locus_tag="ACHJ69_001564"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HFO5014570.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAKPVAYVIHGENHAELARQMAHKHACLRHVLVAGETMSDDFTPLFSLHGER
                     QAWPQPDVSTTALLLLSGGTTSTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNTQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDRYRHSAQ"
     gene            33424..35445
                     /gene="fyuA"
                     /locus_tag="ACHJ69_001565"
     CDS             33424..35445
                     /gene="fyuA"
                     /locus_tag="ACHJ69_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HFO5014571.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
     gene            36096..36845
                     /locus_tag="ACHJ69_001566"
     CDS             36096..36845
                     /locus_tag="ACHJ69_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HFO5014572.1"
                     /translation="MSKKYQPLLITHYMSTWVTITEAVEITTKAIKQKITPSDIYRHA
                     LSGNILLSVYFQSPVILKKIQTFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCT
                     EARYICPVQQIIDTPLLRKLNQFRTFVRNVRPGDELDVPAQVSEKNLTPPPGNSSGNL
                     EQQIASTSQLIGSLLAEDMNSEQAANIARGWASSQASGVMTDWLSRFGTARITLGVDE
                     DFSLKNSRDGNPRKWRFDATNSELCAERHEC"
     gene            36772..38894
                     /locus_tag="ACHJ69_001567"
                     /pseudo
     CDS             36772..38894
                     /locus_tag="ACHJ69_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(39064..40116)
                     /locus_tag="ACHJ69_001568"
     CDS             complement(39064..40116)
                     /locus_tag="ACHJ69_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO5014573.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRTPVPMTWAFMMREAGFPAVRSIYELPLSDDVLDEVREEMRSLGSYIALNLDGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVYGPKGEDKARALVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            40431..41747
                     /gene="shiA"
                     /locus_tag="ACHJ69_001569"
     CDS             40431..41747
                     /gene="shiA"
                     /locus_tag="ACHJ69_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO5014574.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRS"
     gene            41849..43303
                     /gene="amn"
                     /locus_tag="ACHJ69_001570"
     CDS             41849..43303
                     /gene="amn"
                     /locus_tag="ACHJ69_001570"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO5014575.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGRGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT        10044 a        12281 c        11419 g         9741 t
ORIGIN      
        1 aaacagcctg gttcattaca aaattgtaat gctgctgtaa ggttaccctg gacgtttttt
       61 cgctatcctc aaaactcatt cacatgacaa ggatataaac atgttaaagc gttatttagt
      121 actctccgta gtaacggctg cattttcatt accttctttg gtctatgctg cacaacaaaa
      181 cattcttagc gtgcacattt tgaaccagca aacaggaaaa cccgctgccg acgtgacagt
      241 cactcttgaa aagaaagcgg ataacggctg gttacaactt aataccgcca aaacagataa
      301 ggatggacga attaaggcac tgtggcccga gcaaactgcg actactggcg attaccgtgt
      361 cgtatttaaa accggggact atttcaagaa acaaaatctt gaaagtttct tccctgagat
      421 ccccgttgaa tttcatatta ataaagtgaa cgagcattat catgtgcctt tgttactcag
      481 ccaatatggg tattcaacct atcgtggcag ttaatgcgta aatatgtaaa agctgatctc
      541 tgctactgtt agggaggtga ctccctgaca gattaacagt aaacggctct tgttggctaa
      601 cgacaaaaaa gtgtgatggc ttatgaaaaa gaatcaattt ttaaaagaat cagatgttac
      661 ggcagagtcg gtattcttta tgaagcgtcg gcaggtgtta aaagcactgg gcatcagcgc
      721 ggctgcactt tctttgcctc acgctgcgca tgccgatctg cttagctggt ttaaagggaa
      781 cgatcgccca cccgcccccg ccggaaaagc gctggagttc agcaagcctg ccgcctggca
      841 aaataacctg ccactgacgc cagcagataa agtttccggt tataacaact tctatgaatt
      901 cgggctggat aaagccgatc ccgccgctaa tgctggtagc ctgaaaaccg atccatggac
      961 actgaaaatc agcggcgaag tggcaaaacc attgaccctc gatcacgatg atttaacccg
     1021 tcgcttcccg ctggaagagc gtatttatcg tatgcgctgc gtggaagcgt ggtcgatggt
     1081 ggtgccgtgg attggttctc cgctacacaa attgctggcg cttgccgaac ccaccagcaa
     1141 tgcgaagtat gtcgctttcg aaacgattta tgcgccggaa cagatgccag gccagcagga
     1201 ccgctttatc ggcggcgggc tgaaatatcc ttatgtcgaa ggattgcgtc tcgacgaagc
     1261 aatgcatccg ctcacactga tgaccgtagg tgtttatggc aaggcgttac caccgcaaaa
     1321 tggcgcgccg gtgcgactga ttgtgccgtg gaaatatggc tttaaaggga ttaaatcgat
     1381 cgtcagtatt aagctgaccc gcgagcgtcc gccaaccacc tggaatctgg cagcgcctga
     1441 cgaatacggt ttttacgcca acgttaatcc gcatgttgat cacccgcgct ggtcacaggc
     1501 taccgaacga tttattggtt caggcggcat tctcgatgtt cagcgccagc caacactact
     1561 gtttaatggt tacgccgacc aggtggcatc gctgtatcgt ggcctggatt tgcgggagaa
     1621 tttctgaatg cgtctgacag caaaacaggt gacatggctg aaagtttgcc tgcatcttgc
     1681 cggattgttg ccgtttctct ggctggtctg ggtgattaat cacggtggac tgggtgccga
     1741 tccggtgaaa gatattcagc attttaccgg tcgcactgca ctgaaatttt tgctggcgac
     1801 cttgttaatc acacctctgg cacgctacgc aaaacagccg ttattgatac gcactcgccg
     1861 cctgttagga ttatggtgct ttgcctgggc gacactgcat ttaaccagtt acgcattgct
     1921 ggagctgggc gtgaataatc tggcgttatt aggtaaggag ttaattaccc ggccttattt
     1981 aacgttagga attatcagct gggttatttt gcttgcttta gcgttcactt caacccagtc
     2041 gatgcagcga aaactcggca aacactggca acagttgcat aacttcgtct atcttgtcgc
     2101 gatcctggcc ccaatacatt atctgtggtc agtgaagatt atttcaccgc agccactcat
     2161 ctatgctggg ctggctgtac tgcttttagc cttacggtat aagaagttgc tttccctttt
     2221 caaccggtta cgcaagcagg cccacaacaa attatctcta taacttcata ttgccgacaa
     2281 acttcgcgtt atcccggccg gatgcggcgc gagcgcccta tccggcccac acagatcgca
     2341 tcaagttatg tttcgtttgt catcaacctt tcatcaccag aataatttat tttatttcaa
     2401 taagatagtt caatacacct gagaatacca tgctctcatt tcctaatgag ttgttgcatt
     2461 ttttcatatg ttacaatata acattacaca tcatgtacat taactctgga ggaaattgtt
     2521 ttggcgattc gtcttcataa actggctgtt gctttaggtg tctttattgt tagcgctcct
     2581 gccttttcgc atggtcatca ttctcatggc aaacccttaa cagaggtcga acaaaaagct
     2641 gctaatggtg tttttgatga taccaatgta caaaaccgaa cgctcagtga ctgggatgga
     2701 gtctggcaat ccgtttaccc tttactgcaa agtggcaaac ttgatcccgt ctttcagaag
     2761 aaagcggatg cagataaaac caaaacattt gctgaaatta aagattatta tcgcaaaggt
     2821 tatgcgacag atatcgaaat gattggcatt gaggacggca ttgtcgaatt ccatagaaat
     2881 aatgaaacaa catcctgtaa atatgattac gatggataca aaatactcac ctataaatca
     2941 ggcaagaaag gcgttcgtta cttattcgaa tgtaaagatc ctgaaagcaa agcccctaaa
     3001 tatatacaat ttagcgatca tataattgca ccacgaaaat catctcattt tcacattttt
     3061 atgggtaatg atacccaaca gtctttattg aatgaaatgg aaaactggcc aacgtattat
     3121 ccctatcagt tgagtagcga agaagtggtc gaggagatga tgtctcattg atttttttgc
     3181 gctgacttca gttatttaac agaacagtgc ctggtagtaa caacaataat ttaccaagac
     3241 aaataagtaa gacaaactca gacacgctca tacttgagtc gtgtcttttt tattatgtct
     3301 gtagaaaaaa actgcgaaaa aggcgaacaa taaatacaca actcagccat acagactagc
     3361 agcgcaaatg tttccaggag caagtcatct gtcatggtaa agctaaccat aaatataggt
     3421 taattttgat gtttaaacct tttttacttg ttagtatgat acaaaggctt tcaaaaaagc
     3481 tgggaaattc aacaactctt ggaaattatg gactttgtcc cgcggcttta atctgacggc
     3541 cgcgttcctt ttttggctca attattgacc ccttctctac gcaacttcag ttcccgccag
     3601 caactttgcg gcagtttcat aggatcaatg tctaaaagaa taatggtgac cgataagaaa
     3661 acgactgaat aactgtagat tttcgctcga aaccttcctg tcagacccat agcgaatcaa
     3721 gtgctgaatg tcacagtatc gaacagaaaa cagtgacgat ctaacccttc aagaatattc
     3781 tacgattgtt ctgtttagga aaagcaaggc gggaagtcgg gagataagtc attgataaag
     3841 tggcggagag agggggattt gaacccccgg tagagttgcc cctactccgg ttttcgagac
     3901 cggtccgttc agccgctccg gcatctctcc gttcagatgg ttgccatgat gccaggaaat
     3961 ttggcatttt aacagtccct gtccgtgcaa ttttgttcaa gtgacgagtt tgcgagcaaa
     4021 acgatgatta agtggccctg gaaagtacaa gaatcagcac atcaaactgc ccttccctgg
     4081 caggaagcac tatcgatccc ccttttaacg tgtctgacag aacaggaaca aagcaaattg
     4141 gtcgctcttg ccgaacgttt tttacagcaa aaacggcttg ttcctttaca gggctttgag
     4201 ctgaattcat taagaagctg ccggatagca cttctatttt gcctgcccgt tctggagtta
     4261 ggactggaat ggctggatgg ttttcatgaa gtcttaattt atcctgcgcc atttgtggtc
     4321 gatgatgaat gggaagacga tatcggtctg gtgcataacc aacgtattgt tcagtcaggt
     4381 cagagctggc agcaagggcc tatcgttttg aactggttgg atatacaaga ttcttttgat
     4441 gcttctggtt ttaacctgat tattcatgaa gtcgctcata agctggacac ccgtaacggc
     4501 gatcgcgcca gcggagttcc ctttatttcg ttgcgtgagg ttgctggctg ggaacacgat
     4561 cttcatgctg caatgaacaa cattcaggaa gaaatcgaat tagttggtga gaatgcggcg
     4621 agcattgatg cttatgctgc cagtgatcct gctgaatgtt ttgccgtact ttctgaatat
     4681 ttctttagcg ccccagaact ttttgctcct cgtttccctt cattgtggca acgtttctgt
     4741 caattttatc aacaagatcc tttgcagaga ctgcatcacg ctaatgatac agactcgttt
     4801 tcggcaacga atgttcatta attaacaact ttgcagatta attaaccaat tgaaatggct
     4861 tatgaaattt agtgttgaca gacaaggtac cgctaagtaa tatgcgcccc gttctcacga
     4921 ttcctctgta gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag
     4981 tccagtcaga ggagccaatt ttctgttttc ttgcatcctt gcgaatcttt atctgatgtt
     5041 gattcaacag gttagtgtga aaactcttct cggtgcattt tgattttacc ctacgcatcg
     5101 ggaaaaattg gtggtcaaat ctggggtcag gttagttcga taatggagtg ccccccatat
     5161 atcccttacc gacgcaaaaa tccgcaccct caagccttct gataaaccct ttaaagtctc
     5221 cgattctcac ggtctgtatc tgcgggtcaa gccgggtggc tcccgccact ggtatctcaa
     5281 ataccgtatt agcggtaaag aatcccgcat tgcgctgggt gcctatccag ccatctccct
     5341 gtctgatgcg cgacagcaac gtgaaggtat ccgtaaaatg ctggcgctga atatcaaccc
     5401 ggtacagcag cgggctgctg tacgtggctc acgaacaccg gagaaagttt ttaaaaacgt
     5461 ggcgctggca tggcataaaa gtaacaggaa atggtcgcag aacaccgccg accgtctgct
     5521 tgccagcctg aacaatcaca tctttccggt catcgggaac ctacctgtat cagaacttaa
     5581 accccgtcat ttcattgacc tgcagaaagg gatcgaggaa aaaggtctgc tggaggttgc
     5641 gtcccgcaca cggcagcacc tgagtaacat aatacgccat gcggtccatc aggagttaat
     5701 cgatacgaac cctgcagcaa accttggcgg cgtgaccaca cctcctgtca gacggcacta
     5761 tcctgccctg ccgctggagc ggctgcctga actgcttgaa cgtattgggg catatcatca
     5821 gggccgtgaa ctgacccggc atgccgttct gctgatgctg catgtgttca ttcgctccag
     5881 tgaactgcgt ttcgcccgct ggtcagagat tgatttcaca aaccgagtct ggacgatacc
     5941 cgcgacgcga gaacccatta ttggcgtgca ttattccggc cgcggggcaa aaatgcgaat
     6001 gccgcatatc gtccccctct cagaacagtc catcgccatt ctgaaacaga ttaaggatat
     6061 caccggtaat aatgaactga tcttccccgg cgaccataac ccgtataagc caatgtgtga
     6121 aaacacggtc aataaggcac tgcgggtgat gggttacgac acgaaaaagg atatctgcgg
     6181 tcacggcttc cgggcaatgg catgcagtgc gctgatggaa tcgggtttat gggcaaagga
     6241 cgcagtagaa cgccagatga gtcatcagga gcgcaatacc gtgcgcatgg cttatattca
     6301 taaggcagag cacctggaag cccgcaaagc gatgatgcag tggtggtcgg attatctgga
     6361 agcatgccga gaatcttatg caccgcctta tacaattggt aaaaataagt ttatcccata
     6421 gtatactaac gaatcccaca ctctggcaaa ttaccaccac ctccacagga ggtggttcta
     6481 tatagacttt tatcaacttc atgacgaaaa acacctattt tatcccaaaa gaagacaata
     6541 gagtcacaga ggaatcaaag agcgactcaa ttgtctttgg acagtctggt tgtgaggtta
     6601 gaaaacagtt actcctacac cattaaatag ggcgcaatgc tcgctaattt ctcccgggtt
     6661 tctgtcagtt cgcgttccgg cgtagattgc gcgatgattc ccgcccccgc ctgtatccag
     6721 cagcgctggc tatcctgaaa tacggaacgc agaactagcg ccgcatcaaa gcgcgtatcg
     6781 tccagcagca ggattgcgcc ggaataaagc tctcgcggcg tcttctcaat ttgcataatc
     6841 gcgttcagag cagcattttt agggatacct gaggcggtaa tcgacggaaa cagcacggta
     6901 aacgcatccc aggcatcctt gttttccgca agctgaccgc tcacgccaga ccccagatgc
     6961 tgaacgctgc cgcgctggcg aaccgacatt aaatcttcaa ccaccacgct gcccggctgg
     7021 caaacagcct ccagttcagc aatagcttct ttgacagaaa ggatatgctc aagcacctct
     7081 ttactgtcgt gcagcagttc tgcctctttc gccttattat gctccgggtt tcccatgcga
     7141 tcgcgggtgc ccgcaagcgg ttcagtgacc actttattgc ccgtcactga catcaccagt
     7201 tccgggctaa agcccagcgc ttcgcgtcct tcctgacgga acataaacga gcgcacaggc
     7261 gtgtttgcct gccgcccgta taacagcgtg gcgggcatat caatccgcga tggcagggga
     7321 atggcgcgcg agacaatcac tttgacatac tcgccacggc ggatctccgc aacggcgcgc
     7381 gcaacctgtt gtttatatgc ctcaccattg agggcggtat ccaccgccag tggtgcgttc
     7441 tgcgtcgttg taccggcctc ttttacccac tcgcacaaac gtcggcaccc gtcggcggag
     7501 tccgcataaa cggtgacatt tcccttttca aaaataagtt cttcacggga aacggttaac
     7561 gtcagcagcg gccactcccc ggcgttaaag gcaatccccc gcgcatgcgc cgcaaagttg
     7621 aacccaacct gtccatatac ccgacgtcct ttcacctgag gatgcgccat aaaacgacgc
     7681 gcgcagtcgg caatggaatc caccgcccat ttttgctccc cagcatcatc aataaatgtc
     7741 gcctgcgttc cgtcggcatt aatgtgcagc cgtgcctggc accctttgcc taaataccaa
     7801 cagggttgac gctcatagac ataacactct tcttctgcga actggcgtaa aacgccagaa
     7861 atcgtcggta gccattgttc ctctggtaac gccagatgta gaaattcact gattttcatc
     7921 ccttacctct ctgtgttatt cccggatcaa tcaattgcgc ttgtcatcag ttctcttgcc
     7981 gaatgcagcc ggatataagc aacccacgcc gccagcaacg tgaatgcccc ggcaaacagg
     8041 aagcaggccg catagccaaa atgttgagca acaacgccac cgataacgcc agccagaatg
     8101 gcgattgccg cgtcagtaca ttgaaagagg gtgaagtcga caccggcctg caaaggcgat
     8161 gaaagcgaca tcagcgtggc gtaaagacag accagcgcgc aggccagcga aatggactgg
     8221 acaatgacca gacactggag aatctgcagc agcagatgac ccggcgccat catgagcgtc
     8281 atcaccaccg ccagcagcgc gatcccctgg acgccataag ccgtcaacag cgctctgccg
     8341 ggtgaggtgt atttcatcag caatccgccg gccagcgttc ctgctatgcc cgctgcaata
     8401 ttgccgccgc tgaacagcgc gcccaatgcg gacatgctca acccatgatc caacaacagc
     8461 ggtgccagaa gaggcagcac aaaccgcatg cctgaattca gcatcaatac cagcagtaag
     8521 cccaggcgcg cctgcttcct ccttagcgca taacctaacg ccggaacatg cgggatagtc
     8581 gctgttcgcg tcggctccgt aatgcgccac agcgggagtg acagcgccat aatcagcacc
     8641 gccatcatca gcatggcgac aggccagccg gatgccgccg acagcatcag gaacaccccg
     8701 ccgccgcaca tcattcccag atagctgccg ccgacctgca cgctgtttcc ccagccgtaa
     8761 cccgtgcggg tcagttgatc gacacaaaag ccgtcgctgg cgatatcaat ggtggaggcg
     8821 accgtgcctg ccaccataaa cagaacaaat aacgcgacga tttgcgtatc ggggaatccc
     8881 ccttctcgcc caaaccagcc aatcgctgcg gcaatagtca ggatcgccgc tagcgcgacc
     8941 tgaccacgca ggattaacat gcgggaacgg cgttcctggc taccgggcgg aagccgccag
     9001 cgctcgatcc acggcgccca aataaacttc agcgcccagg gcagcataaa cagggttgtc
     9061 gcaccggcaa gcgccagcga accgccagca gcgcgcacca gcgcgggtaa ggactgcata
     9121 gacagcgccg ataccagact ctgcgtcaca tagacgccgg caatagcaaa aatcacccgc
     9181 ccggtcgtca acgtcagagg tttcacattc gactgaacat cactcatgca cccactctcc
     9241 agacgcggaa cccccgtcgt cgcgccacac gcgcgcggcc atttgcgcct gccacagcgc
     9301 ctgataacgt ccatgatgtg agagcaattg cgcatgagta ccctgctcaa ccacctgtcc
     9361 ctcttccatc accagaatgt ttccggcccc ggcgatggtg gataaacggt gagcgatgat
     9421 aatcaccgtc cggttgtgaa ccaggttatc gatcgctttt tgcaccgcca gctcgctttc
     9481 aatatccagc gcggcagtcg gttcatcgag aatgacgacc ggcgcgtttt tcaataacgc
     9541 tctggcaatg gaaatccgct ggcgctcgcc gcccgatagc tggccgccca tctctcccat
     9601 tggcgtcagc cagccttgcg gaagacggga aataaactca aggcactgcg ccgcgcgggc
     9661 ggcctcttct acctcctgcc gcgtcgcctg tgggcgcgcg atacggatat tcgccagcag
     9721 cgtgtcatca aacagccaga catcctggaa aacaacagaa atcaggctat tgagctgttc
     9781 cggcgtcagg cggcgaatat cgacgccgcc aatagaaatc tgcccttgct gcggatcggc
     9841 atagcgcatt aacagtttgg tgaccgtagt tttgcctgcg ccggaggcac ccaccagcgc
     9901 gctcatactg gctgccggga acgtcaaaga aacatgatta agcgcgtggc cgtcaccttc
     9961 ttcgtagcga tagctgacgt tgtcaaagcg gatatcgtaa cgttccggca tctcgctttg
    10021 ctctgcgacg ggaggcggtg ctatcgccat aaactgctca atccgttgca gggcgctggc
    10081 gatcagttcc acaaccgagg tgtagctgat aaacatcgcc atcggttctg cgaagcgcat
    10141 aatcatcgcg acggcggcaa tcaaaaaggc gaggttcaga gtgcccgtca ctacccagac
    10201 gatcccggat aacaccacca cctgtaggcc caactccacg acgctggcga tcagcatcgt
    10261 tgcgccagcg ccctgacggt gagtgcgggt ctgtaagttt tccagcgcat tgaaatgcgc
    10321 cagcagcgct cggcttttat cggcatcgct gccgcaggtg cgcaatacca tcatcccctg
    10381 agcaaattca acgatatcgc cgctcagacg ctggtgcgct tcccccagcg tctgcatttg
    10441 tcgccgcatc gccgggcggc gccagtaata aaacggcacc agcagaggga agatcagcaa
    10501 catcaccagc cccagccgcc agtctatcca caatgtcgcc agcgacgccg tcagcggcgt
    10561 gacaatggtg agcagcaaaa tattggctat cgcaataaca taattgaggt tttcatccac
    10621 gctgcccagc agcaaggcgt tcatttcacc cgccctgccg cgctggagct tctccagcgg
    10681 cacgcggcgt aactgctcgc caagtcgcag gcgcaactca tgggtagcct gcgccagatg
    10741 accacggtat tcaaagccca ggccatacca gcgtagcacc agcgtcacaa ttgcggcgac
    10801 gctgaaggcc atagcccagt taagaagttg cggcgcgtct ccccgtaaca gcgcatcaat
    10861 gatcggataa agacaggcaa aggcgatccc ctgcatgaat gccgccagca acagcgccag
    10921 catactgcgt cgcagcatcc tggcctgact gccaacgctg gagataagct ggcgccagat
    10981 gacgcgccag gctaggttat ccgcaggatt attgtctttc attttccacc acctcttcct
    11041 gtgttccacc cggcacccaa tgctgcgcct gctgatagtg ttgccagagc cgttgataca
    11101 ggccgccctg cgccaacagt tgcgtatggt tccccatttc cctgagttgt ccgtcggaaa
    11161 acagcaatat cacatcggcc tgagtcacca ttgagagacg atgcgcgacc atgatgaccg
    11221 tccggccacg catggccgcc gcgagcgcct tgataagcgc cgcttcgttt tccgggtcag
    11281 caaacgccgt cgcctcatcg agcaccagga tggggcgatc ctgcaaaagc gcccgggcga
    11341 tagtaatgcg ctgccgctgg ccgccggaga gaaatacccc acgttccccg actcgagtgt
    11401 tgtaaccttc tggcagagcg ctaataaaat catgggcctg cgccactctg gccgccgcta
    11461 taaccgcctc cagcggcgta tccggcgcgc ccagacgaat gttattggct atcgtgtcgg
    11521 caaaaaggaa gttgtcctga aacacaaacg agagttgctt catcagggtg tccgtctgca
    11581 tatcacgcag atccacgccg ccaatacgga tatggccttt gtccgggtcg gcgtaacgca
    11641 gcagcaagcg cgccacggtg ctttttccgg cgccgcttgg cccgaccagc gccacaattt
    11701 gcccggcagg cacatgaaag ctcacctcct gcaacgcggc gccagtacgc gcttgcggat
    11761 aatgaaagct cacctgctca aaggtaatgc tcgcctcttg cggctgctgg tcagactgcg
    11821 gcagcgataa ctccggcatc gcgagaacct gataaatacg ctgaatgctt aaacgcgttt
    11881 gcgcgaccag gttattgagc atcatcattg gcattacggc ttcggccatt ccgctgccca
    11941 gcagtaaaac ggccacccac gcgataaaat cgaaactgcc atagtgcaac aggccgtatc
    12001 ccgaccagat caggacaaac agggtcggga gaggattcag aatcgagaag gaaaaacgcg
    12061 ctgaaaaacc ggctttgcga taccaggttt tgagcacatc gacccactct tcaagggcgc
    12121 gttgatagcg caaaaaactg gtgctgccgc tatcaaacgt tctcaccacg ggcatggcct
    12181 gcacgaactc aataaccgcc gcgctaacct gctcgcgcgc cttatgataa cgctgagcca
    12241 tattttccga acgctggcgg gcgagcacga gaacaaccga tccaaacgcc agtaccccca
    12301 gcgtcgcgat tgccagccgc cagtccagcc agaacaagat aacgattgtc gccagcggca
    12361 tgatgatcgc gcgggcatag agcggcgtgc tgtcggccac aaaaatatgt aacgacttca
    12421 cgtcatccag catcactttc gccactgacc cgctgcccat ttgctgtaac acgccaggcg
    12481 gaagctgcaa tgctttacgg gctaacctgc tgcgcagaat gttctccaga tgaaaagccg
    12541 cgtaatgaga ggtgttgaac gcctgtaagc gcagaacaaa agttaagacc acgcagccga
    12601 tcacgccgcc cagcggccag gccggaatag cgtcaggcgt cgcgcgaatg tcacgcaggc
    12661 tccacgccag aaaaaggagc gcccccaggc tggttaatgc cgccagcccc gccagcgcca
    12721 tagctaaaat aaccttcctg cgaaccggtg taatcacctg ccagagagga aatcgggaca
    12781 gcgattcagt attggatgat tgagatgaca tgggagtaac tgaatttcct gatgaatttt
    12841 tacggacgcc aaaaacgaca attcagggtg gcgttctgag aattaatgtg aataataacc
    12901 attatcaata actcatctac cccattcggt tctatttata cccgcattgg tctaagccac
    12961 agggagataa ccaggtcatg acggagtcac cgcaaacgca atctgaaatc tctattcacc
    13021 agttggtggt cgggaaacct gcaaacgatg gcaatattcc tgctcagtgt gaactgctgc
    13081 gctgttcgtt gcaggaaggt atggatattt tgctctggag gggccatttt gcgcggccag
    13141 agacgttgca gctacacgac gatctgggca ggattaactt ctcgtgcatc ctggaaggca
    13201 catcgcgctt tgcgattcag ggactccggc gccacaccga ctgggagctt gcgcgcaatc
    13261 gccattacat tacccatacg ccagactgtc gcggcagcgc ctcctattgt ggccgattcg
    13321 agagcattac cctctccttc agccctgaaa ccctcgcgct gtgggtgccg gatattagcg
    13381 cggtcattaa gaataagatc gactcccact gctgttgcca gcagcagcgt tgtaatgctg
    13441 aaacccactt aacggctcag gccttgcgtc acgcgctgat gcgcatgcac ggcggcttca
    13501 gtcatgaaca aaagccgtcg acgctatggt tgcaggggca aagtctggtc atgctcagcc
    13561 tggtgctgga tgaacatcgc gaagacgcca gttgcctctc ctgccacttc aacccgatgg
    13621 aacgccagaa actgctgcgg gcgaaggatc tgctgctggc tgatctgacg caagcgccgg
    13681 gcgtcgcgga gctggccaga gagtccggtc tgagcgttct caaaattaag cgaggcttcc
    13741 gcgttctgtt caacaacagc gtctatggcc tgtttcaggc tgaacgaatg caggaagcca
    13801 gaagacgcct ggccaacggc aatacctccg tcatgacggt ggcggccgat ctgggctatg
    13861 ccaacgccag ccatttctct gccgccttcc agaaacagtt tggcgtaact ccttcgacct
    13921 ttaaacgcgg gatgtaaccg gggtcgcgcc cccctaaact cctccctgac agaggcccgg
    13981 atacctcatc cgggcctctg ttctttccgc ctgttaccaa cccgaaacgg gcataaataa
    14041 acccgtttcg ggtagcatcc aattgttaat aattattatt ctcatatgag taatgctttt
    14101 cggtaagacg tgccatcagg aggaagaatg atttctggcg caccatctca ggattcgctg
    14161 ttaccggaca accgccacgc ggctgattac caacaattac gcgagcggct catacaggaa
    14221 ctgaatttaa cgccgcagca gttacatgaa gagagcaacc tgatccaggc cggcctggat
    14281 tccataagat tgatgagatg gttacactgg tttcgtaaaa atggctaccg ccttaccctt
    14341 cgcgagctgt atgccgcccc cacgctggcg gcatggaacc agttaatgct cagccggtcg
    14401 ccggagaacg cggaagaaga aacgccgccc gacgaatcat cctggccgaa catgaccgaa
    14461 agtaccccct tcccattgac gccagtacag cacgcctacc tgacgggccg catgccgggg
    14521 cagacgcttg gcggcgtggg ttgccacctg tatcaggagt ttgaaggcca ttgtctgacg
    14581 gcgtcgcagc tggagcaggc catcacgacc ttgctgcaac gccacccaat gctgcatatc
    14641 gcctttcgcc ccgacgggca gcaggtctgg ctaccgcaac cttactggaa cggcgtcacc
    14701 gttcatgatt tacgccataa cgacgctgaa agccgccagg cctatctgga cgcactgcgc
    14761 cagcgcctga gccaccgtct tttacgcgtg gaaatcggcg aaacgtttga ttttcagctg
    14821 acgctcttgc cggacaatcg ccaccgcctc catgtcaata ttgacctgct gattatggat
    14881 gcctccagct ttacgctttt cttcgatgag cttaacgccc tgctggccgg agaatcgctg
    14941 ccggctatcg acacccgcta tgatttccgc tcgtatttgc tgcaccagca gaagatcaat
    15001 caaccactga gagacgacgc gcgcgcttac tggctggcga aagcatcgac gcttcccccc
    15061 gcgcccgtct tgccgctggc ctgcgaaccc gccacgctac gtgaagtccg taatacccga
    15121 cgccgcatga ttgtcccggc aacacgctgg cacgccttta gcaaccgggc cggcgagtat
    15181 ggcgtgacgc cgacaatggc actggcgacc tgtttttctg ccgtgctggc tcgctggggc
    15241 ggcctgacgc gtctgctgct taacatcacc ttattcgacc gccagccgct gcacccggcg
    15301 gttggcgcga tgcttgccga cttcaccaat attcttctgc tggataccgc ctgcgatggc
    15361 gataccgtca gcaacctggc gcgtaaaaac cagctcacgt ttacggagga ctgggagcat
    15421 cgccactggt ccggcgtcga attactccgt gaactcaaac gccagcagcg ctacccccat
    15481 ggcgccccgg tggtatttac cagcaatctg gggcgttccc tctacagcag ccgcgcagaa
    15541 tcgccgttgg gcgagccgga atggggcatc tcgcaaacgc cgcaggtctg gatagatcat
    15601 ctggcgttcg agcatcacgg cgaggtctgg ctacaatggg acagcaacga cgcgctgttc
    15661 cctccggcgt tagtcgaaac attgttcgac gcctactgcc agttgattaa ccaactctgc
    15721 gatgacgaaa gcgcctggca aaagccgttc gcagatatga tgcccgccag ccagcgcgcg
    15781 atacgcgaac gggtcaacgc caccggcgcc cccattcccg aaggcttgct gcatgaaggc
    15841 attttccgta tcgctctgca acagccgcag gcgctggcgg taacggacat gcgttatcag
    15901 tggaattatc atgagctgac agactatgcc cgccgttgcg cgggcaggtt aatcgagtgc
    15961 ggggttcagc ccggcgataa tgtggctatc acgatgtcga aaggcgcagg acaacttgtt
    16021 gcggttctgg ccgtcctgct ggccggggcg gtttacgttc cggtttcgct ggatcagcct
    16081 gccgcacggc gcgagaaaat ctacgctgac gccagcgtcc ggctggtgct catttgtcag
    16141 cacgacgcca gcgccgggtc agacgatatt cccgtccttg cctggcagca ggccattgag
    16201 gcggagccga tcgccaaccc ggtagtacgc gcccccacgc aaccggccta cattatctac
    16261 acctccggct ctaccggtac gccgaaaggg gtagtcattt ctcaccgggg agcgcttaac
    16321 acctgttgcg atatcaatac ccgctatcag gttggcccgc atgacagggt gctggccctc
    16381 tccgccctac attttgattt atcggtttac gacatttttg gcgtactgcg cgcgggcggc
    16441 gcgctggtga tggtgatgga aaatcaacgg cgcgatcctc acgcatggtg tgagctgatc
    16501 cagcgccatc aggtcacgct ctggaacagc gtcccggcgc tgttcgatat gctgctgacc
    16561 tggtgtgaag gtttcgccga cgccacgccg gaaaacctgc gcgcagtgat gctttccggc
    16621 gactggatcg ggcttgacct ccccgcccgt tatcgggcct tccggccaca aggacaattt
    16681 atcgcgatgg gcggcgccac cgaggcgtct atctggtcta acgcctgcga aattcacgac
    16741 gtccccgccc actggcgctc catcccttac ggttttccgc taaccaacca acgctaccgg
    16801 gtggtggatg aacagggccg ggactgccct gactgggtgc cgggtgaatt atggattggc
    16861 ggcattgggg tcgcggaagg ctatttcaac gatcccctgc gtagcgagca gcaatttttg
    16921 acgctcccgg acgagcgctg gtatcgcacc ggcgatctcg gctgctactg gccagatggc
    16981 acaatcgagt tcctcggtcg tcgcgacaag caggtcaaag tcggaggata tcgcatcgag
    17041 ctgggcgaaa tcgaaagcgc gctcagccag ctggcggggg tgaaacaagc aaccgttctg
    17101 gcgatcggcg aaaaagaaaa aacgctggcg gcatacgttg ttcctcaggg cgaggctttt
    17161 tgcgttaccg atcatcggaa cccggcactg ccgcaggcgt ggcacacgct tgcgggaacg
    17221 ttgccctgtt gcgccatctc gccagagatc tccgcagaac aggtagccga tttccttcag
    17281 catcgcctgc taaaactgaa gccgggtcac accgctggcg ccgatcctct ccccctgatg
    17341 aactcactcg ctatccagcc gcgctggcag gccgtggtgg aacgctggtt agcatttctg
    17401 gtgacacaac ggcgactgaa gcccgctgct gaaggttatc aggtctgcgc tggtgaagaa
    17461 cgcgaggatg agcacccgca cttcagcgga catgatttaa cgttatcgca aattcttcgc
    17521 ggtgcccgta acgaactgtc gttactgaac gacgcgcagt ggtcgccgga aagcctggcc
    17581 tttaaccatc cggccagcgc cccgtatatt caggaactgg cgacaatttg ccaacagctt
    17641 gcacagcgct tacagcgccc ggtacgcctg cttgaggtgg gaacccgcac tggccgcgcc
    17701 gcagaatcgc tgttagcaca gctcaacgcc ggacagattg agtatgtcgg gcttgagcag
    17761 agccaggaga tgctgctgag cgcccggcag aggctcgccc cctggcctgg cgcccgtctg
    17821 tccctctgga atgcagacac gctggcgacg cacgctcact cggcggacat tatctggctt
    17881 aataacgccc tgcatcgtct gctgccggaa gatcccgggc tccttgcgac attacaacag
    17941 cttgccgttc ccggcgcgct gctctacgtg atggagtttc gccagttaac gccgtccgcc
    18001 ctactcagca cgctcctgtt aaccaatggg cagccggagg ccttgctgca taacagcgcc
    18061 gactgggcgg cattatttag cgcggccggc ttcaactgtc agcatggcga tgaggtcgcg
    18121 gggttacaac gcttcctcgt acaatgtcct gacaggcagg tgcgccgcga tccccgtcaa
    18181 cttcaggccg ccctcgccgg gcgtctgccg gggtggatgg tgccgcaacg gatcgtattc
    18241 ctcgacgcct taccgctgat ggctaacggg aaaattgact accaggcgct gaagcgtcgt
    18301 catacccctg aagcggaaaa cccggccgaa gcggatttac cccagggcga cattgaaaaa
    18361 caggttgccg ccctctggca gcaactctta tcaacaggca atgtcaccag agaaaccgac
    18421 ttcttccagc aaggcggcga tagcctgctg gcgacccgtc tgaccgggca acttcatcag
    18481 gcaggttatg aagcgcaatt aagcgacctg tttaatcatc cccggctggc ggattttgcc
    18541 gccacgctgc ggaaaaccga cgtcccggtc gaacaaccat tcgtccactc ccctgaagat
    18601 cgctaccagc cctttgcgct taccgacgtg cagcaggctt acctggtggg gcgtcagccg
    18661 ggctttgccc tgggcggcgt cggctcacat ttctttgttg aatttgaaat tgccgatctg
    18721 gacctcaccc ggctggagac ggtctggaac cgattaatcg cccgccacga tatgctgcgc
    18781 gccatcgtgc gtgatggaca gcaacaggtg ctcgaacaga cgcccccttg ggtgataccc
    18841 gcacacaccc tccatacgcc tgaagaggcg ttgcgggtgc gcgaaaaact ggcgcatcag
    18901 gtactcaacc ccgaagtgtg gccggtattc gatctccagg tcggatacgc ggacgggatg
    18961 cctgcccgcc tgtggctgtg tctggataac ctgttgcttg acggtctgag catgcagatc
    19021 ctgctggcgg agctggagca cggctaccgc tacccgcaac agctgcttcc gccgctgccc
    19081 gtcaccttca gggattatct gcaacaaccc tcgctacagt cgcccaatcc agattctctg
    19141 gcatggtggc aggcgcagct tgatgatatt cctccggcgc ctgcgttgcc gctgcgctgc
    19201 ttgcctcagg aggttgaaac accgcgcttc gcccgcctga acggcgcact ggacagcacg
    19261 cgctggcatc ggctgaaaaa acgggcggct gacgcccatc tcaccccgtc ggccgtactg
    19321 ttgtcggtgt ggtcaacggt tctctctgca tggagtgcac agcctgagtt cacgcttaac
    19381 cttacgcttt tcgacaggcg accgctgcac ccgcaaatca accagattct gggcgatttc
    19441 acctcgctga tgctgctgag ctggcatccc ggcgaaagct ggctgcacag cgcgcagtca
    19501 ctacagcagc ggctgagcca gaacctcaac caccgcgatg tgtcagccat ccgcgtgatg
    19561 cgtcaactgg cgcaacggca aaacgtgcct gccgttccga tgcccgtcgt ctttaccagc
    19621 gcactgggct ttgagcagga taacttcctc gcccggcgta atctgctcaa accggtctgg
    19681 ggcatctccc agacgccgca ggtctggctc gatcaccaga tttatgaatc cgaaggcgaa
    19741 ctgcgcttta actgggattt tgtcgccgcg ctgtttcctg ccgggcaggt ggagcgccag
    19801 tttgaacagt attgcgcatt gctaaaccga atggccgagg atgaaagcgg ctggcaactg
    19861 ccgctcgccg cgctggtgcc tcccgttaaa cacgcagggc aatgcgcaga gcgctcaccg
    19921 cgcgtatgcc ctgagcactc tcagccacac attgcggcgg acgagagcac cgtcagcctg
    19981 atttgcgacg ccttccgcga ggtggttggc gagtctgtca cgcccgcaga aaacttcttt
    20041 gaggcgggcg caacgtcgct gaatctggtg caactgcacg ttttgttaca acgtcacgaa
    20101 ttttccaccc tgacgttgct tgacctcttc acccaccctt ctcctgctgc cctggccgat
    20161 tatctggccg gcgtcgccac ggtggagaaa acaaaacgac ctcgccctgt tcgccgtcgt
    20221 cagcggcgga tatagcgcga agcaaactga ttttccccgg aacgccatcg cgaacgcatg
    20281 gcgttccatt gactttgtga atcttaggaa acgggaccga ttatggataa cttgcgcttc
    20341 tcttctgcgc cgacagcaga ttccattgat gcatcgatcg ctcaacacta cccggactgc
    20401 gaacctgtcg cggttatcgg ctacgcctgc cattttcctg aatcgccgga tggcgaaacg
    20461 ttctggcaaa atctgctgga aggtcgtgaa tgcagccgac gctttacgcg cgaagagctt
    20521 ctggccgtcg gtctggatgc cgccatcatt gacgatcctc attatgtcaa tatcggtacg
    20581 gtgttagaca acgccgactg cttcgacgcc accctgtttg gctattcgcg acaggaagcg
    20641 gagtcgatgg acccgcagca gcgcctgttt ttgcaggcgg tctggcatgc gctggaacat
    20701 gccggttatg cccccggcgc cgtcccccat aagaccggcg ttttcgcctc ttcccggatg
    20761 agtacctacc ccggtcgcga agcattgaac gtgacagaag tcgcgcaggt aaaaggtctg
    20821 caatctctga tgggcaatga taaagactat attgccaccc gcgccgcgta caaactcaac
    20881 ctgcacggcc cggcgttatc ggtacagacc gcctgctcca gctcgctggt tgccgtgcat
    20941 ctggcctgtg aaagcctgcg cgcaggcgaa tccgatatgg cggtggccgg cggcgtggcg
    21001 ctctctttcc cccagcaggc aggctaccgc taccagcccg gaatgatttt ctctcctgat
    21061 ggtcactgtc gtccctttga cgcctcggct gagggcacct gggccggtaa cggtctcggc
    21121 tgcgtggtgc tgcgtcgcct gagagacgcg ctgctgtcag gcgatccgat tatctcggtg
    21181 atcctctcca gcgcggtcaa caacgacggc aacagaaagg tcggctatac cgccccttcc
    21241 gtcgcagggc aacaggcagt catcgaagag gcgttaatgc tggcggccat cgacgacagg
    21301 caggtaggtt acattgaaac ccacggcacc ggcacaccgc tgggcgacgc gattgaaatt
    21361 gaagcgttac gcaacgtcta tgcgcctcgc ccgcaggatc agcgctgtgc gctcggttcc
    21421 gtgaaaagta acatgggcca tctggatacc gcggcgggca ttgccggact gctgaaaacc
    21481 gttctggcag tcagtcgcgg gcaaattcct cccttactga attttcacac ccccaacccg
    21541 gcgctgaaac ttgaagagag cccctttacc ataccggtgt cggcacaggc atggcaggac
    21601 gaaatgcgct atgcgggcgt ctcctccttt ggtattggcg gcaccaactg ccatatgatc
    21661 gtcgcctcgc tgcccgacgc gctcaacgcg cgcctcccca atacggatag cggcagaaaa
    21721 agtaccgcgc tgctgctcag cgccgccagc gacagcgcgt tgcggcggct ggcgacggat
    21781 tatgccgggg cgctgagaga gaatgcggat gccagctctc tggccttcac agccctgcac
    21841 gcgcgccgtc tcgatctccc cttccgcctg gcggcgccat taaaccgtga aaccgccgag
    21901 gcgctcagcg cctgggccgg tgagaaatcg ggggcgctgg tttacagcgg ccacggcgcc
    21961 agcggcaagc aggtgtggct gtttaccggc cagggctcgc actggcgcac tatgggtcaa
    22021 acgatgtacc atcactcaac ggcgtttgcc gacacgctgg atcgctgttt ttccgcctgt
    22081 agcgaaatgc tcacgccgtc actgcgcgaa gcgatgttta accccgattc ggcgcagctg
    22141 gacaatatgg cctgggcgca gccggcgatt gtcgcgtttg aaatcgcgat ggcggcgcac
    22201 tggcgtgctg aaggactgaa gccagacttc gccattgggc attccgtcgg tgaatttgcc
    22261 gctgccgttg tctgcggaca ctatacgatt gaacaggtca tgccactggt ttgtcggcgc
    22321 ggcgcgctaa tgcagcagtg cgcaagcggc gcgatggtgg cggtatttgc agacgaagac
    22381 acgctgatgc cgctggctcg ccagtttgag ctggatctcg ccgccaacaa cggtacgcaa
    22441 catacggtat tttccgggcc ggaagcccgt ctcgcggtat tttgcgccac gctctcgcag
    22501 catgacatta actatcgtcg cctgagcgta accggtgcgg cgcactccgc tttactggag
    22561 ccgatactcg atcggttcca ggacgcctgc gcgggactgc acgcggagcc ggggcaaata
    22621 ccgattattt ccacgctcac cgccgacgtc attgatgagt caacgctcaa ccaggcggat
    22681 tactggcgcc gacacatgcg ccagccggtg cgttttatcc agagtattca ggtggcgcat
    22741 cagctcggcg cccgcgtttt tctggagatg gggcccgatg cccagttggt tgcttgcggg
    22801 cagcgcgaat accgcgataa cgcatactgg atagccagcg cccggcgtaa caaagaggcg
    22861 agcgatgtcc tcaatcaggc cctgctccag ctttacgctg ccggcgtcgc cctaccgtgg
    22921 gccgacctgc tggcgggcga tggacaacgt atcgctgcgc catgttatcc gtttgatact
    22981 gaacgttact ggaaagagcg cgtctccccg gcctgcgagc ctgccgacgc agcgctgtct
    23041 gccgggctgg aggtggcgag tcgcgccgcg acagcgctcg atctccctcg cctggaagcg
    23101 cttaaacagt gcgccacgcg actgcacgcc atctacgtcg atcaactggt acaacgctgt
    23161 accggcgatg ccattgagaa cggcgtggac gccatgacca tcatgcgccg tggacgtctg
    23221 ctgccccgct accagcagct actccagcgc ctgctgaata actgcgtggt cgacggcgat
    23281 taccgctgca ccgacgggcg atacgtccgc gcccgcccca ttgaacatca acagcgggaa
    23341 tcactgctga cggaacttgc cggttattgt gaaggttttc aggctattcc cgacaccatc
    23401 gcccgtgccg gcgatcggtt atatgaaatg atgagcggcg cggaagaacc ggtggcgatt
    23461 atcttcccgc aaagcgcctc cgacggcgtg gaagtgctgt atcaggaatt cagctttggc
    23521 cgctatttca accaaatcgc cgccggggta ttacgcggca ttgtccagac gcgtcagccc
    23581 cgccagccgt tgcgtattct tgaagttggc ggcggaaccg gcggcaccac cgcgtggctg
    23641 ctgccggaac tcaacggcgt tccggcactg gagtaccatt tcaccgatat ctcggcgctg
    23701 ttcacccgtc gcgcccagca gaaattcgcc gactacgatt ttgtgaagta tagcgagctg
    23761 gatctcgaaa aagaggcgca gtctcagggt ttccaggcac agtcttacga tcttatcgtg
    23821 gcagcgaacg tgattcacgc cacccgccat attggccgca cgctcgataa tctgcgcccc
    23881 ctgctcaagc caggcgggcg cctgctgatg cgcgaaatca cccagccaat gcgtctgttt
    23941 gacttcgttt tcggcccgct ggttcttccg ctacaggatc tcgacgcccg cgaaggtgag
    24001 ttattcctca ccaccgctca gtggcaacaa cagtgccgcc acgccggatt cagcaaagtg
    24061 gcgtggctac cgcaggatgg cagcccgaac gccgggatga gcgaacatat cattctcgcc
    24121 acgctgcccg gtcaggcggt tagcgccgta acattcaccg cgccatcaga acccgtgttg
    24181 gggcaggcgc tgacggataa cggtgattat ctcgccgact ggtctgattg cgcaggtcag
    24241 cccgaacagt ttaacgcccg ctggcaggag gcatggcgtc tgctttcaca gcgtcatggc
    24301 gacgctctgc ctgtggaacc gccccccgtc gccgccccgg agtggctggg gaaggttcgc
    24361 ttaagctggc aaaacgaagc cttttcccgc ggtcagatgc gcgttgaagc ccgtcatcct
    24421 gctggcgagt ggctgccgct atcgcccgcc gcgcctcttc ctgcgccgca aacgcattat
    24481 caatggcgct ggacgcccct caacgtcgcc agcattgacc atccgcttac ctttagcttc
    24541 agcgccggta cgcttgcgcg cagcgacgag ctggcgcaat acggcatcat tcacgatccg
    24601 cacgcctctt cacgactgat gattgttgag gagagcgagg atacgctggc cttagcggag
    24661 aaagtgatag cagcgctcac cgccagcgca gccggattga ttgtggttac tcgccgcgcg
    24721 tggcgagtcg aggaaaatga agcactctct gcatcccatc acgcgctatg ggccttgctt
    24781 cgcgtcgcgg ccaacgaaca gccggaacgg ttgcttgccg ccatcgatct cgccgaaaac
    24841 accccgtggg aaacgctgca tcaagggttg agcgcagtct cactaccaca gcgctggctc
    24901 gccgcacggg gtgacaccct ttggcttcct tcactgtcgc ccaatacggg atgcgccgct
    24961 gaattaccgg caaacgtgtt taccggcgat agccgctggc atctggtgac cggagcgttt
    25021 ggcggattag gccgccttgc cgtgaactgg ctcagagaaa aaggggcgcg acgcatcgcc
    25081 ctgctggcgc cgcgcgtgga tgagtcatgg ctacgcgacg tggagggcgg gcagacgcgc
    25141 gtctgccgtt gtgatgtggg cgatgccggg caactggcca cggttcttga cgatctggcg
    25201 gccaacggcg gcattgccgg agcgattcat gccgctggcg tattggctga cgcgcccttg
    25261 caggagcttg atgaccacca gctggctgcc gttttcgcgg taaaagcgca ggcggcaagc
    25321 cagctgttgc aaaccctgcg caaccacgac ggacgctatc ttattctcta ctcttccgct
    25381 gccgccaccc tcggcgcgcc gggtcagagc gcccatgcgc tggcctgcgg ctacctggac
    25441 gggctggccc agcagttttc cacccttgat gcgccgaaaa cgctctctgt cgcctggggc
    25501 gcatggggag aaagcggtcg ggcggccacg ccggaaatgc tggcgacgct cgccagccga
    25561 ggtatgggcg cgttaagcga tgccgaaggc tgctggcacc tggaacaggc ggtgatgcgc
    25621 ggcgccccgt ggcgactggc gatgcgcgtt tttaccgaca aaatgccccc gttacaacag
    25681 gctctgttta acatcagcgc cacagaaaaa gccgcaacgc cggtcattcc tcctgctgat
    25741 gacaacgcct ttaacggcag cctgagcgat gaaacagcgg tgatggcatg gctgaaaaag
    25801 cggattgcgg ttcagctaag gctgagcgat ccggcgtcac tgcatccaaa ccaggatctg
    25861 ttgcaactcg gcatggactc gctgctcttc cttgaactca gtagcgatat tcagcactac
    25921 ctgggtgtac gcatcaatgc ggaacgggcg tggcaggatc tgtctcctca tggactcacg
    25981 cagcttatct gttctaagcc agaggcgacg cctgccgctt cgcagccgga agtgttgcgg
    26041 cacgacgccg acgagcgtta tgcgcccttc cctttgacgc ccattcagca cgcctactgg
    26101 ctggggcgaa cccacttcat tggctatggc ggcgtcgcct gtcacgtcct gtttgagtgg
    26161 gataaacgcc acgatgagtt cgatctcgcc atactggaga aagcatggaa ccagctcatc
    26221 gcacgccacg atatgttgcg tatggtggtt gatgccgacg ggcagcagcg aatcctggcg
    26281 acaacgccgg agtatcacat cccgcgtgac gatctgcgcg cgctttcccc ggaagaacag
    26341 cgcatcgcgc tggaaaaacg gcggcatgaa ctgagctatc gcgttttgcc tgccgaccag
    26401 tggcctcttt ttgagctggt ggtcagcgaa atcgacgatt gccattaccg tctgcatatg
    26461 aacctcgacc ttttgcagtt tgatgtgcag agttttaaag tcatgatgga cgacctggcg
    26521 caggtctggc gcggtgaaac gctggcaccg ctcgctatta ccttccgtga ttatgtgatg
    26581 gctgaacagg cgcgccgaca gacatcggca tggcacgatg cctgggatta ctggcaggaa
    26641 aaactgccgc aactgccctt agcgccagag ctgccggtgg ttgagacgcc cccggaaacg
    26701 ccacacttca ccaccttcaa atcgacgatc ggcaagacag aatggcaggc cgtgaaacag
    26761 cgctggcagc agcaaggcgt cacaccgtct gccgcgctgc tcacgctgtt tgccgccacc
    26821 cttgagcgct ggagccgtac cacaacattt acgctgaacc tgacgttctt caatcgccag
    26881 ccgatccatc cgcaaatcaa ccagttgatt ggtgatttta cctccgtcac gctggttgat
    26941 tttaacttct cagcgccggt gacgttgcaa gagcagatgc aacagaccca acagcgcctc
    27001 tggcaaaaca tggcgcacag tgaaatgaac ggtgttgagg tgatccgtga gctgggccgc
    27061 ctgcgcggat cacaacgtca accgctgatg ccgatagtgt ttaccagtat gctggggatg
    27121 acgctggaag gcatgactat cgatcaggcg atgagccatc tgttcggcga accctgctat
    27181 gtattcacgc aaacgccgca ggtctggctg gatcatcagg tcatggagag cgacggcgag
    27241 ttgatgttta gctggtactg catggacaac gtgctggaac ccggcgctgc cgaggcgatg
    27301 tttaatgact attgcgccat cctgcaagcc gtcatcgccg cccctgaaag cctgaagact
    27361 ctcgccagcg gcatcgccag gcacattccc cgccgacgct ggccgctgaa cgcgcaggcg
    27421 gactacgacc tgcgggatat tgagcaggcg acgctcgaat accccggcat ccggcaggcc
    27481 agagcggaaa taaccgaaca gggcgcgttg acgctggata tcgtgatggc cgacgatcct
    27541 tcgccatcag cggcgatgcc tgatgagcac gaacttaccc aactggcgct gccgttgcct
    27601 gagcaggcgc agcttgatga gctggaggcg acctggcgct ggctggaggc gcgtgcgcta
    27661 caggggatcg cggctacgct aaatcgtcac ggcctgttta ccacgccgga gatcgcccat
    27721 cgctttagcg caatagtaca ggcgctgtcc gcgcaagcgt ctcaccagcg tctgctgcgc
    27781 cagtggctac agtgtctgac ggaaagagag tggttaatcc gcgaaggtga aagctggcgc
    27841 tgccgcattc cgctcagcga gattcctgag cctcaggaag cgtgcccgca aaaccaatgg
    27901 agccgggcgc tggcgcagta tctggaaacc tgcatcgccc ggcacgacgc ccttttctcc
    27961 gggcagtgtt ctccgctgga attgctgttc aacgagcagc atcgcgttac cgacgcgctg
    28021 tatcgcgaca accccgccag cgcctgtctg aatcgctata ccgcgcagat tgccgccttg
    28081 tgcagcgcag aacggattct ggaggttggc gccggaaccg cagccactac cgcgccggtg
    28141 ctgaaggcca cgcggaacac gcgacagtcg taccacttca cggacgtctc cgcgcagttc
    28201 ctcaatgacg ccagagcccg tttccatgat gaatcgcagg tgtcttatgc cttgttcgac
    28261 atcaaccagc cgctggattt caccgcccac ccggaggcgg gttacgacct gatcgttgcc
    28321 gtcaatgtgc tccacgacgc cagccatgtc gtccagacgt tgcgcagatt aaaactgttg
    28381 ctgaaagccg gcggacgttt gctgatcgtt gaagcgacgg agcgaaacag cgtattccag
    28441 ctggcgagcg tgggctttat tgagggatta agcggatacc gcgatttccg ccgccgggat
    28501 gagaaaccga tgctcacccg ctccgcatgg caggaggttc tcgttcaggc cgggtttgca
    28561 aacgagctgg cgtggcccgc gcaggaatcg tcgccgctgc gccagcatct gctggtagcg
    28621 cgttcgcctg gcgtaaatcg cccggataaa aaagccgtga gccgctattt acagcagcgc
    28681 tttggcaccg gtctgcccat tttacagatc cggcaaagag aagcgttatt tacgccgctg
    28741 catgccccgt ctgatgcgcc gactgagcca gccaaaccca cgccagttgc cggggggaat
    28801 ccggcgctgg aaaaacaggt ggctgaactc tggcaatcgc tgctgtctcg ccccgtggca
    28861 aggcatcacg actttttcga actgggcggc gacagcctga tggcgacaag gatggtcgcg
    28921 cagctaaacc ggagagggat tgccagggct aaccttcagg atctgttcag ccattcgacg
    28981 ctgagcgact tctgcgccca tctacaggcg gctacgtcag gagaggacaa cccgataccc
    29041 ctttgccagg gcgacggtga ggaaaccctg tttgtcttcc acgcttcaga cggcgatatc
    29101 agcgcctggc tgccgctcgc tagcgcgttg aacaggcgcg ttttcggcct gcaagcaaaa
    29161 tcgccgcagc gctttgccac gctcgaccag atgatcgatg agtatgtcgg gtgcatccgt
    29221 cgtcagcagc ctcacggccc ttatgtgctg gcgggttggt cgtatggcgc gtttcttgcg
    29281 gcgggcgccg cacagcgcct gtacgccaaa ggcaagcagg ttcggatggt gttaatcgat
    29341 cccgtgtgcc gacaggattt ctgttgcgaa aaccgggcgg ccctgctgcg cctgttagcc
    29401 gaaggacaaa cgcctctggc actgcccgaa catttcgacc agcagacgcc cgacagccag
    29461 cttgccgact ttatcagcct cgctaaaacg gccggtatgg tgtcgcaaaa cctgacgctg
    29521 caagcggcag aaacgtggct cgacaacatc gcgcatctgc tgcgtttact gactgagcat
    29581 acgccgggcg aaagcgttcc ggtcccctgt ctcatggtgt atgccgccgg gagacccgag
    29641 cgctggacgc cagcagaaac cgagtggcag ggccggataa acaacgccga cgacgctgtg
    29701 attgaagcca gccactggca aatcatgatg gaagcccccc acgttcaggc ttgtgcgcaa
    29761 cacattacgc gctggctttg cgcaacctca acgcaaccgg agaacacgtt atgatgccgt
    29821 ccgcctcccc aaaacaacgt gtactgattg tgggcgccaa atttggcgaa atgtacctga
    29881 atgcctttat gcagcccccg gaggggctgg aactggtcgg cctgctggcg cagggaagcg
    29941 cccgttcaag agagctggct catgcgtttg gcattccgct gtatacctcg ccggaacaga
    30001 taaccaggat gccggatatc gcctgtattg tcgtgcgttc gaccgtcgcc ggaggaaccg
    30061 gtacgcagct tgccagacac tttctgacgc gcggcgtaca cgtcattcag gagcatcccc
    30121 tccacccgga tgacatcagt tccttacaaa cgctggcgca ggaacagggc tgctgctatt
    30181 gggtaaacac gttttatccg catacgcgcg cgggacgcac atggcttcgc gatgcgcagc
    30241 agctgcgtcg ctgcctggct aagacgccgc cggtagtcca cgccaccacc agccgacagt
    30301 tgctctactc cactctggat ttgttgttgc tggctctggg cgttgatgcc gccgccgtgg
    30361 agtgcgacgt ggttggcagc tttagcgatt ttcactgtct gagactcttc tggccggagg
    30421 gagaagcctg cctgctgctt cagcgctatc tcgatcctga cgatccagat atgcatagcc
    30481 ttatcatgca ccgcctgctg ctgggctggc cggaaggtca tctttcgctg gaggcgagct
    30541 acggccccgt catctggtca tccagcctgt ttgtcgcaga ccatcaggag aacgcccaca
    30601 gcctctggcg cagaccggag atcctgcgcg atctgccggg tctgacgcgc agcgcggcgc
    30661 ccctgagctg gcgcgactgc tgcgaaaccg tcgggccgga gggcgtcagc tggttgctac
    30721 accagttgcg cagccatctg gcgggcgaac atccaccagc ggcctgccaa agcgtacatc
    30781 agatcgcgtt atcacgttta tggcagcaga ttttacgtaa gaccggcaat gcggagatca
    30841 gacgcctgac gccgccgcac cacgaccggt tagccggttt ctacaacgat gatgataagg
    30901 aggcgctgtg acgcaatctg caatgtgcat accgctgtgg cccgcccgga acggcaatac
    30961 tgcgcatctg gtcatgtgcc ctttcgctgg cggcagcagt agcgcgtttc gccactggca
    31021 agctgagcaa ctggctgatt gcgcgctttc tctggtgacc tggccggggc gcgatcgcct
    31081 tcgccatctg gaaccgctca gaagcattac acaactggcg gcactgctgg cgaacgagct
    31141 ggaagcatcc gtatcgcctg acacgccgct tttactcgcc gggcacagca tgggggcgca
    31201 ggtggcgttt gaaacctgcc gacttctgga gcaacggggg cttgcgccac aaggactgat
    31261 tatttccggg tgccatgccc cgcatctgca ttctgaacgc cagctcagcc atcgcgatga
    31321 tgccgacttt atcgctgagc tgatagacat tggcggatgt tctcctgaac tgcgggaaaa
    31381 ccaggaatta atgtcgctgt ttcttcctct tctgcgcgct gatttttacg ccaccgagag
    31441 ctatcactac gactcgcccg acgtctgtcc gccgctgcgc acgcctgcgc tgttattgtg
    31501 cggcagccac gatcacgaag cctcctggca gcaggtcgat gcctggcgtc agtggctgag
    31561 ccacgttaca ggcccggtgg tgattgacgg cgatcacttc tatcccattc aacaagcccg
    31621 gtcctttttt acgcagattg tccgccattt tccccacgca ttttctgcaa tgaccgcgtg
    31681 gcaaaaacag cccagtactt cagaaaggtg acgcatgaat tcttcctttg aatctctgat
    31741 tgaacagtat cccttaccca ttgccgaaca gttgcgccac tgggcggccc gttatgcctc
    31801 gcgaattgcc gtcgttgatg caaaggggtc gttaacctac agcgcgcttg atgcacaagt
    31861 tgacgaactt gccgcaggtc tgtcatcact gggtttgcgt tcgggggagc atgtaattgt
    31921 gcagcttccc aacgacaacg cgtttgttac cctgctgttc gccttgttaa gactgggcgt
    31981 tatccccgtg ctggcgatgc cctcgcaacg ggcgctggat atcgacgcgc tgattgagct
    32041 ggcgaaaccc gtcgcttacg ttattcacgg ggaaaaccac gcagagctgg cccgacagat
    32101 ggcgcacaaa cacgcctgct tgcgtcatgt tctggtcgct ggagagacca tgagcgacga
    32161 ttttacgccg ctcttctccc ttcacggtga gcgacaggca tggccgcagc ctgatgtttc
    32221 caccaccgcg ttgttgttgc tctcaggcgg cacaaccagc acgcccaaac tcatcccgcg
    32281 ccgacatgcc gactatagct ataacttcag cgcttctgct gaactgtgcg gcatcagcca
    32341 acagagcgtg tatctcgccg tcctcccggt ggcgcataac tttccgctgg cctgccccgg
    32401 tattctggga acccttgcct gcggcggaaa agtggtgctg accgacagcg ccagctgtga
    32461 tgaggtgatg cctttaatcg cgcaggaaag agtgactcac gtcgccctgg ttccggcgct
    32521 ggcgcaatta tgggtgcagg ccagggagtg ggaagacagc gacctttcgt cgctacgcgt
    32581 cattcaggca ggcggcgccc ggctcgaccc gacgcttgct gagcaggtta tcgccacctt
    32641 tgactgtacc ctgcaacagg ttttcggtat ggcggaaggc ctgctctgtt ttacccggct
    32701 ggacgatccg catgccacca ttctccacag ccaggggcgc ccgttgtccc ctctggatga
    32761 aatccgcatc gttgatcaag acgagaacga cgtcgcgccg ggcgaaaccg ggcaattgtt
    32821 aacgcgcggc ccttatacca tttcgggcta ttaccgcgcc cctgcccaca acacgcaggc
    32881 ctttaccgcg caagggtttt accgcacagg cgacaatgtc aggctggatg aggtggggaa
    32941 cctgcacgtt gagggacgca taaaagagca gatcaaccgc gccggagaaa aaatagccgc
    33001 ggctgaagtg gaatcggcac tgctgcgttt agcggaagtg caagattgcg cggtggtcgc
    33061 cgcgccggac acgctgcttg gcgagcggat ttgcgcgttt atcatcgcgc agcaggtgcc
    33121 aactgactat cagcagttgc gtcaacaact gacccgtatg gggctcagcg cgtggaaaat
    33181 tcctgaccaa atcgagtttc tggaccactg gccgctcacc gccgtcggca agatagacaa
    33241 aaaacgcctg acggctctcg ccgtcgaccg ttatcgccat tctgcccaat aagcgcaaac
    33301 cgacccgaaa caggttgaaa taaacccgtt tcgggtagca ccaccattag aaatagttat
    33361 cattttcaat tcaccattgt cggtattttt tggcgtttcg ccgtcttaca gggactcaca
    33421 acaatgaaaa tgacacggct ttatcctctg gccttggggg gattattgct ccccgccatt
    33481 gctaatgccc agacttcaca gcaagacgaa agcacgctgg tggttaccgc cagtaaacaa
    33541 tcttcccgct cggcatcagc caacaacgtc tcgtctactg ttgtcagcgc gccggaatta
    33601 agcgacgccg gcgtcaccgc cagcgacaaa ctccccagag tcttgcccgg gctcaatatt
    33661 gaaaatagcg gcaacatgct tttttcgacg atctcgctac gcggcgtctc ttcagcgcag
    33721 gacttctata accccgccgt caccctgtat gtcgatggcg tccctcagct ttccaccaac
    33781 accatccagg cgcttaccga tgtgcaaagc gtggagttgc tgcgaggccc acagggaacg
    33841 ttatatggca aaagcgctca gggcgggatc atcaacatcg tcacccagca gccggacagc
    33901 acgccgcgcg gctatattga aggcggcgtc agtagccgcg acagttatcg aagtaagttc
    33961 aacctgagcg gccccattca ggatggcctg ctgtacggca gcgtcaccct gttacgccag
    34021 gttgatgacg gcgacatgat taaccccgcg acgggaagcg atgacttagg cggcacccgc
    34081 gccagcatag ggaatgtgaa actgcgtctg gcgccggacg atcagccctg ggaaatgggc
    34141 tttgccgcct cacgcgaatg tacccgcgcc acccaggacg cctatgtggg atggaatgat
    34201 attaagggcc gtaagctgtc gatcagcgat ggttcaccag acccgtacat gcggcgctgc
    34261 actgacagcc agaccctgag tgggaaatac accaccgatg actgggtttt caacctgatc
    34321 agcgcctggc agcagcagca ttattcgcgc accttccctt ccggttcgtt aatcgtcaat
    34381 atgcctcagc gctggaatca ggatgtgcag gagctgcgcg ctgcaaccct gggcgatgcg
    34441 cgtaccgttg atatggtgtt tgggctgtac cggcagaaca cccgcgagaa gttaaattca
    34501 gcctacgaca tgccgacaat gccttattta agcagtaccg gctataccac cgctgaaacg
    34561 ctggccgcat acagtgacct gacctggcat ttaaccgatc gttttgatat cggcggcggc
    34621 gtgcgcttct cgcatgataa atccagtaca caatatcacg gcagcatgct cggcaacccg
    34681 tttggcgacc agggtaagag caatgacgat caggtgctcg ggcagctatc cgcaggctat
    34741 atgctgaccg atgactggag agtgtatacc cgtgtagccc agggatataa accttccggg
    34801 tacaacatcg tgcctactgc gggtcttgat gccaaaccgt tcgtcgccga gaaatccatc
    34861 aactatgaac ttggcacccg ctacgaaacc gctgacgtca cgctgcaagc cgcgacgttt
    34921 tatacccaca ccaaagacat gcagctttac tctggcccgg tcgggatgca gacattaagc
    34981 aatgcgggta aagccgacgc caccggcgtt gagcttgaag cgaagtggcg gtttgcgcca
    35041 ggctggtcat gggatatcaa tggcaacgtg atccgttccg aattcaccaa tgacagtgag
    35101 ttgtatcacg gtaaccgggt gccgttcgta ccacgttatg gcgcgggaag cagcgtgaac
    35161 ggcgtgattg atacgcgcta tggcgcactg atgccccgac tggcggttaa tctggtcggg
    35221 ccgcattatt tcgatggcga caaccagttg cggcaaggca cctatgccac cctggacagc
    35281 agcctgggct ggcaggcgac tgaacggatg aacatttccg tctatgtcga taacctgttc
    35341 gaccgtcgtt accgtaccta tggctacatg aacggcagca gcgccgtcgc gcaggtcaat
    35401 atgggtcgca ccgtcggtat caatacgcga attgatttct tctgattatt gtaaaaggga
    35461 taccgaaaag gtatcccttt tacaccacta gttaaaacca gtaactcagc agagttgcaa
    35521 aaaatattaa tccatagtga ttatttaaac aatgaaattg cgattaggat aaatagattt
    35581 aactttctcg ttcctttctc tccttatact aaagaaataa tcatatcaaa ataaaaattc
    35641 acaacagtgc aacattaaaa aatacaacca acaaacaatc ctatatacaa ggcacatctc
    35701 cagaatataa aagcacagac aaacaaccta aaaaaacaac cccatacata taaattattt
    35761 tataggtaaa atagattata tattctcata gctaccacaa actatactag cctgaactat
    35821 atttattctg ctgcaatcaa tgcatacata acacaaatat cactcaggta cactactcaa
    35881 accacgctgg gatttttcct caagttataa tcatcacccc gaaaatcatt cggcatttac
    35941 tcattaaata gtcaccccat aggcctgtac atgtttactc agaaatatac atccttttct
    36001 ctgtcataaa ccctctgatt aatcataaat aaatacttgt gacaccaatc tttttcctta
    36061 acggaacgaa ttgttgtgta gaaggagata atattatgtc caaaaaatat cagccattgc
    36121 ttataactca ttatatgtca acatgggtca ctataacgga agcagttgag atcactacca
    36181 aagccataaa acaaaaaatt actcctagcg atatttatcg tcatgccttg agtggcaaca
    36241 tcctactatc ggtctatttc cagtctcctg tgatacttaa gaagatacaa acttttaatg
    36301 gaaaaataaa attcaggcag tttgtgggag acctacttga taaactatgc atgcttgaca
    36361 gggacggttt tatttatggt caaaatttaa gactctgtac agaagccaga tatatttgtc
    36421 ctgtgcaaca aattatagat acccccctgt tacgcaaact caaccagttt cgtacgtttg
    36481 ttcgtaatgt ccgcccgggt gatgaactgg atgtcccggc acaagttagt gaaaaaaatt
    36541 taaccccgcc gccgggtaac agcagtggca accttgagca acagatagcc agtacttcac
    36601 agctaattgg atctctgctc gccgaagata tgaacagcga gcaagcggca aacatagcac
    36661 gtggatgggc ctcttctcag gcttcagggg taatgacaga ctggttaagc cgcttcggta
    36721 ccgcaagaat cacgctgggc gtggatgaag atttcagcct gaagaactcc cgtgacggca
    36781 accctcgaaa atggcgattc gatgcaacaa acagtgaact atgtgccgaa cgtcacgaat
    36841 gctgaaatca tgctggcagc ctcgaaggat ccggtgattg ccgacaataa cgatctcacg
    36901 acactaacag caccgtcgct gatacagagg gcaatgcgat agccaacact gaggtaacat
    36961 ttactctacc ggaagatgtg aaggcgaact tcacgctgag cgatggcgac aaagcggtta
    37021 ctgatgccga tggcaaagcg aaagtcacgc tgaaaggtac aaaagcaggc gctcataccg
    37081 tgacagcatc gatggttggc ggtaagagtg agcagttggt ggtgaacttt actgcggata
    37141 cgctcactgc gcaggttaat cttaacgtta ccgaggacaa tttcatcgcc aataacattg
    37201 gaatgaccag gctacaggca acagtgactg atggaaacgg caacccagta gaaggtataa
    37261 aagttaattt ccgcggaacc tccgtcacgc taagcagcac cagcgttgaa acggatgatc
    37321 aggttttcgc tgaaattctt gtgacaagca ccgaggtcgg actaaaaaca gtttcagcct
    37381 ctctggcaga taaacctact gaagtcatct cgcgattact gaatgccaag gtagatgtta
    37441 attctgcgac gattaccagt caggagatac ctgaaggtca ggtcatggtc gcacaagaca
    37501 tagcagttaa agctcacgtt aacgaccagt ttggcaaccc ggtgacgcat caacccgcga
    37561 cattcagtgc agcgccatcc tcgcaaatga tcatcagcca gaatacggtc tctactaata
    37621 cgcagggagt agccgaggtc accatgacgc ctgaaagaaa cggctcgtat actgtaaaag
    37681 catccctagc gaatggagct tcacttgaga aacaactgga ggctattgat gaaaaactga
    37741 cactcacgtc cagtccgctt atcggtgtca atgctcctaa aggcgctact ctgacggcaa
    37801 cgctaacctc tgcaaatggc actccagtgg agggtcaggt catcaacttt agcgtaacgc
    37861 tagaaggtgc gacgttaagt ggcggaaaag tgagaaccaa ctcttcaggt caggctccag
    37921 tcgttctgac cagcaataaa gtcggtacat atacggtgac tgcatcgttc cataacggcg
    37981 tcacaataca gacacagaca accgtgaagg ttactggcaa cccaagcacc gcacatgttg
    38041 ctagcttcat cgctgatccg tcgactatcg ccgccaccaa cagtgattta agtaccttaa
    38101 aggcaacggt tgaggatggc agtggtaacc tgatcgaagg tctcactgtg tacttcgcct
    38161 taaaaagcgg ctccaccaca ttaacgtcat taacagcggt gaccgatcaa aacggaatcg
    38221 cgacaacaag cgtgaaaggt gagataacgg ggagcgtcac tgtaagcgca gtcacaagcg
    38281 caggtggaat gcaaacagta gatatatcgc tagtggcagg cccagcagac gcctcgcagt
    38341 ccatccttaa gaacaaccag tcatcactga aaggggactt taccgatagt gctgagctac
    38401 atcttgttct gcacgatata tcaggcaatc cgatcaaagt ttcttaaggg atagaatttg
    38461 tgcaatcagg tacaaacgtg ccctatatga aaattagcgc aattgattac agtcaaaata
    38521 tcaacggtga ttacaaagct actgttacag gaggcggaga gggtatcgca acgctgatcc
    38581 ccgtattgaa tggtgttcat caggcgggtc tgagtaccac gatagaattc attagcgcgg
    38641 aaaccagacc aatgacgggt acagtgtcag tgaatagtgc aaacctaccg acagcttcat
    38701 tcccttcgca gggatttacc ggggcgtatt atcagttgaa taatgacaac tttgcaccag
    38761 gaaaaacggc ggctgattat tcgttttcaa gctcggcctc ctgggtaggc gttgatgcta
    38821 ctggtaaagt gacattcaaa aatgacggtg atagcaatac tgtaataatc acagcgcccc
    38881 caagatccgg tggtgctatt taccaaacag ttcctcctga atcgcgttcg gtgtgactat
    38941 gatcattttc ggtgagtatt gctttgatcc aaaggctggt tttctgtgat gattgaccag
    39001 cctcagagta ttgatgacac caccgttcat aacagatcgg cattactatg cataagtact
    39061 gcctcaaatt ctcgccaaaa cactttttaa tgcctttgcg aattcatcca gacttatatt
    39121 gtcaattttt tgcccaacga cgactgactc cgaaacatcg gccattgcgg gccatcgcgt
    39181 tttgtaatca gcataaattg caacaacagg ggtattatag gcacttgcca tatgtaatat
    39241 tgaggtgtca ggagttattg caatataagc atcttttatg attgctgctg aacgtttaat
    39301 cgagggtgat aaggataaac ggtagacatt attataacaa tccactaatg ccctggcttt
    39361 atcttcccct ttgggtccat aaacgatcac tattggaata tctgtttcac tttgaatttt
    39421 tgctattaga ttttctgcaa tcgataatga aaatgtacgt tcctgcgagc taccgtctaa
    39481 attgagcgca atgtaagatc ctaacgatcg catttcctcg cgtacctcat cgagtacatc
    39541 atcgcttaat ggcaattcat atattgacct gactgccgga aaacccgcct cacgcatcat
    39601 aaatgcccat gtcatgggta caggagtccg gagattctga tccatccggg acgcgttctt
    39661 acacaaaggg gaatagcact tcatggttaa accgacgacc tggaaattcg tttttgcttt
    39721 caactgactg ataaatatca tcgttttcag gttcttttta cgcatcgcct ctatacaaag
    39781 atccggcgta ccgtattgca gtgttatgtt cttcgctaaa tcttttattt cttttaatgt
    39841 tgcgtgatcc tgcatagtca ttgtgactaa tgttaattta gtctgttcaa gtttgagcgc
    39901 attaaagact tctaaattaa ttgtcgacgt aacaattaat agatgcttaa ttttatgcaa
    39961 ttcaagcgcc cgaataacag gaaagatggc catagcatcg ccaatctgat cgggaatatg
    40021 tatgacaaca aagtctgttt tttcaatatt aaaattataa gctttataat cgtagtaact
    40081 aaatgcaata cgtctcaaca atgatactaa aaacataact aacctcgcct ccctactggt
    40141 tataatgcaa tgcagtctat cagaatcatc agggtgcaat tttgtgcata tgtggacttt
    40201 tatgtttcat atctcgaacc tgtgggccct ctgcttaatc cttaaacaac accagcaact
    40261 cctgcgcttt catcttccat cgaatttttc atgttgccgc taatcagcca taaaatcatt
    40321 tgcagatgcg ctctgtcgag gtagtctcat aaggttcgtt tatagatcga cggcaatgtg
    40381 agttaccttt tccatactaa ttataaaaag acagtacaaa caggatcatt atggactcca
    40441 cgctcatctc cactcgtccc gatgaaggga cgctttcgtt aagtcgcgcc cgacgagctg
    40501 cgttaggcag tttcgctggt gccgtcgtcg actggtatga ttttttactc tatggcatta
    40561 ccgccgcact ggtgtttaat cgcgagtttt tcccgcaagt aagcccggcg atgggaacgc
    40621 tcgccgcatt tgccaccttt ggtgtcggct tccttttccg accgctcggc ggtgtcattt
    40681 tcggtcactt tggtgaccgg ctgggacgta agcgcatgtt aatgctgacc gtctggatga
    40741 tgggcatcgc gacagccttg attggtattc ttccttcatt ctcgaccatt gggtggtggg
    40801 cacctatttt gctggtgaca ctgcgtgcca ttcagggatt tgccgtcggc ggcgaatggg
    40861 gaggcgcagc gttgctttcc gttgaaagtg cgccgaaaaa taaaaaagcc ttttacagta
    40921 gcggtgtaca agttggctac ggagtaggtt tactgctttc aaccggactg gtttcattga
    40981 tcagtatgat gacgactgac gaacagtttt taagctgggg ctggcgcatt cctttcctgt
    41041 ttagcatcgt actggtactg ggagcattgt gggtgcgcaa tggcatggag gagtccgcgg
    41101 aatttgaaca acagcaacat aatcaagcgg ccgcgaaaaa acgcatcccg gttatcgaag
    41161 ctctgttacg acatcccggt gctttcctga agattattgc actacgactg tgcgagttgc
    41221 tgacgatgta cattgttact gcctttgcac ttaattattc aacccagaat atggggttac
    41281 cgcgcgaact tttccttaat attggtttgc tggtaggtgg attaagctgc ctgacaattc
    41341 cctgttttgc ctggcttgcc gatcgttttg gtcggcgcag ggtttatatc acaggcgcgt
    41401 tgatcggaac gttgagcgca tttcctttct ttatggcgct tgaagcacaa tctattttct
    41461 ggatagtttt cttctccata atgctggcaa acattgcgca tgacatggtg gtgtgtgtgc
    41521 aacaaccgat gtttaccgaa atgtttggtg ccagttatcg ctacagtggt gctggagtcg
    41581 gttatcaggt tgccagtgtg gttggcggtg gatttacacc ttttattgcc gctgcactca
    41641 tcacttactt tgccgggaac tggcatagcg tcgccattta tttgctggct ggatgcctga
    41701 tttctgcaat gaccgcttta ttgatgaaag acaatcaacg ctcgtgatag cctggcgaag
    41761 atcatccgat cttcgcctta tacttttgtt tcacattcct gtgacatact atcggatgtg
    41821 cggtaattgt atggaacagg agacacacat gaataataag ggctccggtc tgaccccagc
    41881 tcaggcactg gataaactcg acgcgctgta tgagcaatct gtagttgctt tacgcaacgc
    41941 cattggcaaa tatattacaa gtggcgaatt acctgatgaa aacgcccgca aacaaggtct
    42001 ttttgtctat ccatcactga ccgtaacctg ggacggtagc acaactaatc cccccaaaac
    42061 gcgcgcattt ggtcgtttta ctcacgcagg cagctacacc accacgatta ctcgccctac
    42121 tctctttcgt tcgtatctca atgaacaact tacgttgctg tatcaggatt atggtgcgca
    42181 tatctctgtg caaccctcgc agcatgaaat cccttatcct tatgtcattg atggctctga
    42241 attgacactt gatcgctcaa tgagcgctgg tttaactcgc tacttcccga caacagaact
    42301 ggcgcaaatc ggcgatgaaa ctgcagacgg catttatcat ccaacggaat tctccccgtt
    42361 atcgcatttt gatgctcgcc gggttgattt ttccctcgca cggttgcgcc attataccgg
    42421 tacgccagta gaacattttc aaccgttcgt cttgtttacc aactatacac gttatgtgga
    42481 tgaattcgtt cgttggggat gcagccagat cctcgatcct gatagtccct acattgccct
    42541 ttcttgtgct ggcgggaact ggatcactgc cgaaaccgaa gcgccagaag aagccatttc
    42601 cgaccttgca tggaaaaaac accagatgcc agcatggcat ttaattaccg ctgatggtcg
    42661 gggtattact ctggtgaata ttggcgtggg accgtccaat gctaaaacca tctgcgatca
    42721 tctggcagtg ctacgcccgg atgtctggtt gatgattggt cactgtggcg gattacgtga
    42781 aagccaggcc attggagatt atgtccttgc acacgcttat ttacgcgatg accacgttct
    42841 tgatgcggtt ctgccgcccg atattcctat tccgagcatt gctgaagtgc aacgtgcgct
    42901 ttatgacgct accaagttag tcagcggcag gcctggtgag gaagtcaaac agcggctgcg
    42961 tacaggtact gtggtaacca cagatgacag gaactgggag ttacgttact cagcttctgc
    43021 acttcgtttt aacttaagcc gggccgtagc aattgatatg gaaagtgcaa ccattgccgc
    43081 gcaaggatat cgtttccgcg tgccatacgg gacactactg tgtgtttcag ataaaccgtt
    43141 gcatggcgag attaaacttc ccggtcaggc taaccgtttt tatgaaggtg ctatttccga
    43201 acacctgcaa attggcattc gggcgatcga tttgctgcgc gcagaaggcg accgactgca
    43261 ttcgcgtaaa ttacgaacct ttaatgagcc gccattccga taataagttc tgaaccttct
    43321 catcagaaat agatgtgaag gattaagtaa gacatctggc aaatagcctg caatcaacgc
    43381 gggctgtttg cttccttggg cagatgcgag tttcattatc gtcttaatga tttccgcata
    43441 ttaaaagcat gtatgctttc aaaatataat tataaaaaat cccgc
//
LOCUS       DAZFFE010000024        42741 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFFE010000024 DAZFFE010000000
VERSION     DAZFFE010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.024"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1073
                     /locus_tag="ACHJ69_001571"
     CDS             <1..1073
                     /locus_tag="ACHJ69_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO5014576.1"
                     /translation="GLDNDNNNRFDKSSATLKNQGIEARDEVKYLQHYIDELRDKVDL
                     TVALVHEGVPARQSSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVGTPEPIKVGN
                     TLILSTDSGGIDIGKLILDVNPTARTHKVKSFELKTVYADEWIPDPTTQKVINGWNKK
                     LADLVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSGSLRADINAG
                     TITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQMSKGAEMRYTLQKPV
                     GQRIVSFKINGEEIVDTNIYHVATTTFLALGGDGFLAFKEGKNVQVRAGNNMSNVVID
                     YFKKGHKITPAQVNEMRVEVSK"
     gene            1150..2010
                     /locus_tag="ACHJ69_001572"
     CDS             1150..2010
                     /locus_tag="ACHJ69_001572"
                     /inference="COORDINATES: protein motif:HMM:NF011686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO5014577.1"
                     /translation="MYIKKHWIALSILLIPCIGNAQEIKIDESWLHQSLNVIGRTDSR
                     FGPRLTNDLYPEYTVAGRKDWFDFYGYVDLPKFFGVGSHYDVGIWDEGSPLFTEIEPR
                     FSIDKWTGLNLAFGPFKEWFIANNYVYDMGDNQSSRQSTWYMGLGTDIDTGLPIKLSA
                     NIYAKYQWQNYGAANENEWDGYRFKIKYSIPLTNLFGGRLVYNGFTNFDFGSDLADKS
                     QNNKRTSNAIASSHILSLLYEHWKFAFTLRYFHNGGQWNAGEKVNFGDGPFELKNTGW
                     GTYTTIGYQF"
     gene            complement(2228..4660)
                     /gene="metL"
                     /locus_tag="ACHJ69_001573"
     CDS             complement(2228..4660)
                     /gene="metL"
                     /locus_tag="ACHJ69_001573"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO5014578.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(4663..5823)
                     /gene="metB"
                     /locus_tag="ACHJ69_001574"
     CDS             complement(4663..5823)
                     /gene="metB"
                     /locus_tag="ACHJ69_001574"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO5014579.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPREH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQSDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            6100..6417
                     /gene="metJ"
                     /locus_tag="ACHJ69_001575"
     CDS             6100..6417
                     /gene="metJ"
                     /locus_tag="ACHJ69_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO5014580.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            6477..7085
                     /gene="yiiX"
                     /locus_tag="ACHJ69_001576"
     CDS             6477..7085
                     /gene="yiiX"
                     /locus_tag="ACHJ69_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO5014581.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDSSFSWSDDRQYCSEVVWKVYQNALGIRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(7146..7358)
                     /gene="rpmE"
                     /locus_tag="ACHJ69_001577"
     CDS             complement(7146..7358)
                     /gene="rpmE"
                     /locus_tag="ACHJ69_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO5014582.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            7561..9759
                     /gene="priA"
                     /locus_tag="ACHJ69_001578"
     CDS             7561..9759
                     /gene="priA"
                     /locus_tag="ACHJ69_001578"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO5014583.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQRKIWRDQVAELEF
                     NDAALQALRKKGLCDLASETPEFSDWRKNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILASPLADEKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            9915..10940
                     /gene="cytR"
                     /locus_tag="ACHJ69_001579"
     CDS             9915..10940
                     /gene="cytR"
                     /locus_tag="ACHJ69_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO5014584.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHNRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            11032..11991
                     /gene="ftsN"
                     /locus_tag="ACHJ69_001580"
     CDS             11032..11991
                     /gene="ftsN"
                     /locus_tag="ACHJ69_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO5014585.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELENRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASSQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            12084..12614
                     /gene="hslV"
                     /locus_tag="ACHJ69_001581"
     CDS             12084..12614
                     /gene="hslV"
                     /locus_tag="ACHJ69_001581"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO5014586.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            12624..13955
                     /gene="hslU"
                     /locus_tag="ACHJ69_001582"
     CDS             12624..13955
                     /gene="hslU"
                     /locus_tag="ACHJ69_001582"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO5014587.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            14022..14948
                     /gene="menA"
                     /locus_tag="ACHJ69_001583"
     CDS             14022..14948
                     /gene="menA"
                     /locus_tag="ACHJ69_001583"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO5014588.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGAVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            15041..15526
                     /gene="rraA"
                     /locus_tag="ACHJ69_001584"
     CDS             15041..15526
                     /gene="rraA"
                     /locus_tag="ACHJ69_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO5014589.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGASGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(15611..15856)
                     /gene="zapB"
                     /locus_tag="ACHJ69_001585"
     CDS             complement(15611..15856)
                     /gene="zapB"
                     /locus_tag="ACHJ69_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO5014590.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            16281..17126
                     /gene="glpF"
                     /locus_tag="ACHJ69_001586"
     CDS             16281..17126
                     /gene="glpF"
                     /locus_tag="ACHJ69_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO5014591.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            17149..18657
                     /gene="glpK"
                     /locus_tag="ACHJ69_001587"
     CDS             17149..18657
                     /gene="glpK"
                     /locus_tag="ACHJ69_001587"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO5014592.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            18828..19838
                     /gene="glpX"
                     /locus_tag="ACHJ69_001588"
     CDS             18828..19838
                     /gene="glpX"
                     /locus_tag="ACHJ69_001588"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO5014593.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            19935..20681
                     /gene="fpr"
                     /locus_tag="ACHJ69_001589"
     CDS             19935..20681
                     /gene="fpr"
                     /locus_tag="ACHJ69_001589"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO5014594.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(20686..21114)
                     /gene="uspD"
                     /locus_tag="ACHJ69_001590"
     CDS             complement(20686..21114)
                     /gene="uspD"
                     /locus_tag="ACHJ69_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO5014595.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(21141..21440)
                     /gene="yiiS"
                     /locus_tag="ACHJ69_001591"
     CDS             complement(21141..21440)
                     /gene="yiiS"
                     /locus_tag="ACHJ69_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO5014596.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(21652..22092)
                     /gene="yiiR"
                     /locus_tag="ACHJ69_001592"
     CDS             complement(21652..22092)
                     /gene="yiiR"
                     /locus_tag="ACHJ69_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO5014597.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFIPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            22193..22792
                     /gene="yiiQ"
                     /locus_tag="ACHJ69_001593"
     CDS             22193..22792
                     /gene="yiiQ"
                     /locus_tag="ACHJ69_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1454 family protein"
                     /protein_id="HFO5014598.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQITWLPIQGPEQKAAKAKAQAYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            22900..23667
                     /gene="tpiA"
                     /locus_tag="ACHJ69_001594"
     CDS             22900..23667
                     /gene="tpiA"
                     /locus_tag="ACHJ69_001594"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO5014599.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(23722..24477)
                     /gene="cdh"
                     /locus_tag="ACHJ69_001595"
     CDS             complement(23722..24477)
                     /gene="cdh"
                     /locus_tag="ACHJ69_001595"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO5014600.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEQCLP
                     NQQENQNPSPCAEVKPNAGYVVLKDRHGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSQKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(24584..25573)
                     /gene="sbp"
                     /locus_tag="ACHJ69_001596"
     CDS             complement(24584..25573)
                     /gene="sbp"
                     /locus_tag="ACHJ69_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO5014601.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(25893..26855)
                     /gene="pfkA"
                     /locus_tag="ACHJ69_001597"
     CDS             complement(25893..26855)
                     /gene="pfkA"
                     /locus_tag="ACHJ69_001597"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO5014602.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(27036..27938)
                     /gene="fieF"
                     /locus_tag="ACHJ69_001598"
     CDS             complement(27036..27938)
                     /gene="fieF"
                     /locus_tag="ACHJ69_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO5014603.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(28087..28587)
                     /gene="cpxP"
                     /locus_tag="ACHJ69_001599"
     CDS             complement(28087..28587)
                     /gene="cpxP"
                     /locus_tag="ACHJ69_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO5014604.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEIAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            28737..29435
                     /gene="cpxR"
                     /locus_tag="ACHJ69_001600"
     CDS             28737..29435
                     /gene="cpxR"
                     /locus_tag="ACHJ69_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO5014605.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALSLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            29432..30805
                     /gene="cpxA"
                     /locus_tag="ACHJ69_001601"
     CDS             29432..30805
                     /gene="cpxA"
                     /locus_tag="ACHJ69_001601"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO5014606.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(30976..31650)
                     /gene="yiiM"
                     /locus_tag="ACHJ69_001602"
     CDS             complement(30976..31650)
                     /gene="yiiM"
                     /locus_tag="ACHJ69_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO5014607.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTDLGLEGDEQ
                     AEKKIHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWEK"
     gene            complement(31799..32782)
                     /gene="kdgT"
                     /locus_tag="ACHJ69_001603"
     CDS             complement(31799..32782)
                     /gene="kdgT"
                     /locus_tag="ACHJ69_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO5014608.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARVAKIEILGTVK"
     gene            complement(33042..33662)
                     /gene="sodA"
                     /locus_tag="ACHJ69_001604"
     CDS             complement(33042..33662)
                     /gene="sodA"
                     /locus_tag="ACHJ69_001604"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO5014609.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     NLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVQKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            33948..34982
                     /gene="rhaT"
                     /locus_tag="ACHJ69_001605"
     CDS             33948..34982
                     /gene="rhaT"
                     /locus_tag="ACHJ69_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO5014610.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGSGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(34979..35827)
                     /gene="rhaR"
                     /locus_tag="ACHJ69_001606"
     CDS             complement(34979..35827)
                     /gene="rhaR"
                     /locus_tag="ACHJ69_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO5014611.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(35901..36737)
                     /gene="rhaS"
                     /locus_tag="ACHJ69_001607"
     CDS             complement(35901..36737)
                     /gene="rhaS"
                     /locus_tag="ACHJ69_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO5014612.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            37025..38494
                     /gene="rhaB"
                     /locus_tag="ACHJ69_001608"
     CDS             37025..38494
                     /gene="rhaB"
                     /locus_tag="ACHJ69_001608"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO5014613.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQHGQRVGLPVAY
                     RDSRTHGLMAQAQQQLGKRDIYQRSGIQFLPFNTIYQLRALTEQQPELIPHIAHALLI
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            38491..39750
                     /gene="rhaA"
                     /locus_tag="ACHJ69_001609"
     CDS             38491..39750
                     /gene="rhaA"
                     /locus_tag="ACHJ69_001609"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO5014614.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGLLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQITPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDRIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRTYEKEILSRRG"
     gene            39935..40759
                     /gene="rhaD"
                     /locus_tag="ACHJ69_001610"
     CDS             39935..40759
                     /gene="rhaD"
                     /locus_tag="ACHJ69_001610"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO5014615.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADITP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQILVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            40769..41083
                     /gene="rhaM"
                     /locus_tag="ACHJ69_001611"
     CDS             40769..41083
                     /gene="rhaM"
                     /locus_tag="ACHJ69_001611"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO5014616.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKAHNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANADNSPVSSELQEVFY
                     LP"
     gene            complement(41124..42518)
                     /locus_tag="ACHJ69_001612"
     CDS             complement(41124..42518)
                     /locus_tag="ACHJ69_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO5014617.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAEGQVKQLQTQQAAEIREIKTAQGNTPVNGQSTTESEKKNATPPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYNIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKIHGDDS
                     DDRYGARVRFKYFF"
BASE COUNT        10625 a        11101 c        10867 g        10148 t
ORIGIN      
        1 agggactcga taatgacaat aataaccgat ttgataaatc ttccgcgacc ctcaagaatc
       61 aggggattga agcacgtgat gaagtgaagt atttgcagca ttatatcgac gagttacgtg
      121 ataaggttga cctcaccgtg gcgctggttc atgaaggcgt accggcgcgt cagtccagca
      181 ttggcaatac agatgttaga cgtgcgctgg ataaagatat tcaaaccgca agcaaggtga
      241 aaggattgga tattctgatt accgggcatg cccacgtcgg aacgccagaa ccaatcaaag
      301 tcggcaacac cttaatcctt tcgactgaca gtggcggtat cgatattggt aaattaatcc
      361 ttgatgtcaa ccctacagct cggactcata aagtgaagag ctttgagctg aaaacggttt
      421 atgccgatga atggataccc gatccaacca cacaaaaagt tatcaatggc tggaataaaa
      481 aactggcgga tcttgtacgc caaccagtcg gtgaatcatc gattgcatta actcgtgcct
      541 acggtgagtc atcgcaactt ggcaacctgt ttaccgatgc aatgcttgtc gctgcaccga
      601 ctgcacaaat tgcgttgata aactccggca gtttacgtgc tgatataaat gcaggcacga
      661 ttacctttgg tgacattacc agtacattcc ccttcaagaa tgaacttact gaaatggatc
      721 tcagcggtaa ggatctgcgt aacctactgg aacacggggc atcgcttacc aatggcatcc
      781 tgcaaatgtc aaaaggcgca gaaatgcgct ataccctgca gaaaccagta ggtcagcgca
      841 ttgtatcttt taaaattaat ggtgaagaga tcgttgatac gaatatttac catgttgcga
      901 ctacgacttt cctagcactt gggggcgatg gttttctggc attcaaagaa gggaaaaatg
      961 tccaggtccg cgccggaaat aacatgtcca atgtcgtaat cgattatttt aagaaaggcc
     1021 acaagattac tcctgcacag gtaaatgaaa tgcgggtaga agtaagcaaa taacaaaaac
     1081 ttataatacc attcattcac tggatgacat tatttctctc taaatcaaca atatataaga
     1141 gatatattta tgtatataaa aaagcactgg atagctttat ccattctatt aataccttgc
     1201 attggaaacg ctcaggaaat taaaattgat gaaagctggt tacatcaaag cttgaatgtc
     1261 attggtcgca cagactctcg ctttggccca agactgacta acgacctcta ccctgaatat
     1321 actgtagcag gaagaaaaga ctggtttgat ttttatggtt atgttgatct accgaaattc
     1381 tttggcgtcg gcagtcacta tgatgttggg atctgggatg agggctcacc actatttacg
     1441 gaaatagaac ctcggttttc cattgacaaa tggaccggat taaatcttgc gttcggccca
     1501 tttaaagaat ggttcattgc aaacaactat gtctatgata tgggtgacaa ccagtcatcc
     1561 cggcaaagta catggtatat ggggcttggt acagatatcg acacgggtct accaattaag
     1621 ctttctgcca atatatacgc caagtatcag tggcaaaact atggtgccgc taatgaaaat
     1681 gaatgggacg gatatcgatt caaaataaaa tatagcatcc ctcttacaaa tttattcgga
     1741 gggcgattgg tatacaatgg ttttactaac tttgattttg gctccgatct tgcggacaag
     1801 tcacaaaata ataaacgaac cagtaatgct attgcttcaa gccatatcct ttcccttcta
     1861 tatgaacact ggaaatttgc atttacacta cgttattttc acaacggtgg acaatggaat
     1921 gcgggagaga aggttaactt cggagatggt ccatttgaat taaaaaatac aggatgggga
     1981 acctatacta ctattggtta tcaattttaa attaggtgac attttagata ttgttactta
     2041 atgtcatttt gtaattgtca ttaataactt caccattcac tctgttactt catcgctcaa
     2101 cacaaaagcc cccttattac cgggggcact gaccaaaggc aaaatgacaa tcaatcacca
     2161 cttccccagg aagaggttta ggggtgacaa caaaagctgg caactgccag cttttgttca
     2221 ataatcacta caacaactgc gccagtcggt taatatccga ctgaatcgcc ccggcggtga
     2281 cgtcgcgccc tgcacctggc ccgcggatca ccagcgggtt gtcgcgatac cagcggcttt
     2341 cgatggcaaa gacgttatcg catggcagta gtgatgccag cggatggtct tcacgcaccg
     2401 cttccacgcc cacacgcgct ttgccgttgg catcgaaacg cgccacgtaa cgcagcacca
     2461 gccccatttc gcgggccgct tccagtcgtt gcaccatctg ctcgttaagt tcatcgccat
     2521 tttcaaagaa atggtcgatg ctgccgcctt cgcaatgggc aggcaccagt gattcaacac
     2581 gtacctggtc cggttcaatg ttgtaacccg cttcacgcgc cagaatcacc agcttgcgca
     2641 tcacgtcttt gccggaaagg tcatcacgcg ggtcaggctc ggttaagccc tgctgccacg
     2701 cctgatccac cagttcggta aacggcacgc taccgtcgaa ttgcaggaac agccaggaga
     2761 gcgtgccgga gaagatccca ctgatcgaca aaatggtatc gccgctgtcg atcagatcgc
     2821 gcacggtatg gttgatcggc aaacccgcac cgacagtggc attgtacagc cagtgacgcc
     2881 cggttttttc gaaggcgtcg tggatctggc gatatttatt gctgtcgctc gctcctgcca
     2941 gtttgttggc actgatgacg tggaaaccgt ggctggcgaa atcaagatac tggtcagcga
     3001 gctgctggct ggcggtaacg tccagcacca ctaaatcatc atacggatgg gcgcgcatcc
     3061 acaggaacag cgactcttca tcctgctcga ctgcttcatc gttgaagaag gctaacgcgc
     3121 ggctggcgtc cagaccgtca tagctcaaca ggctacggcg gctgtccacc acgcctgcca
     3181 gcacaaattc aaagccggta cgtgccgaaa gcgtactctg ctcacgggcg aacagttcca
     3241 gccagcggga accgatattg cccttgccga acaacaccag gccgatgcgt ttttccgcac
     3301 ggaagacgga ctgatgcagc ccctgaatca ggctttcggt cgggccggtg cgcagtactg
     3361 ccaccaggct gatgccatca tcagactgcc aggtaaactc gaccggctgg cctttcagtt
     3421 gctgccagaa gcggtggcaa tgcagcgggt tacgggtgac gcccgcccca accatcgcca
     3481 ccagcgccag cccctgacgc aggcgcagtt cgccaggtaa tcccgcttcg tcgaggattt
     3541 tcagcgcact gtcggccact tctgaggtgt agcaaaattg cagcaactgg cgatcgttat
     3601 gcacgccaac cgccagtggg cgcacctgag cacgtttcag gatctggtcg atctctttat
     3661 gcgccagttt gaaatcctgg ctggcgggca cctgaaactc aatcaagcag acatcatcat
     3721 ggctggtgac aatacgcgca ccagtaccgg aggccagcac gcgttcaatg cgcgtggaac
     3781 cttgatccgg cgtgtagcta cagcgcagtt gcaggtcgat ttcgctgcca gaaacgggct
     3841 gtaaagtacg ggcatgaaga acgggagccg ccagacgcgc cagttcgctg gcttcatcca
     3901 gacgcagcaa cggcagcagg caggcatctt tcactttacg cgggtcggcg ctatataccc
     3961 cggcgacgtc gctccagatg gttacgcgag aaacacccgc cagcgcaccg atttgtgtcg
     4021 cggaatagtc ggaaccgtta cgccccagca gcaccgtttc accggcgttg ttacggctga
     4081 taaacccggt caccaccaga cgtttgcccg gatgttgtac cagtagctgt tgcagcaacg
     4141 ggtaagaaag tccttcatca acctgcggtt gcgcagcgcg ttcagcgcgt aaaaactcgc
     4201 gggcatcaag ccaggccgct ggcagccctt gttgattaag taccgcagac atcaggcgcg
     4261 ccgaccacac ttcaccgtgg cccaccactt ctgcatacac cgcgtcgtta ataccgctgt
     4321 cgagcagtgc cgccaggcgt tccagatcgc tgacaaaagc gctaatgagg ctatctgctt
     4381 cttcagcggg tagcaggccg ctaatcagat cgcactgata gcgacgtagc gtttgttgaa
     4441 cctgatgcgc agagagacga tcggtctggc ttagtttcaa ccagttaatc aactggttag
     4501 tggtgctacc ggcggcggaa accaccatca tatcgtcagg ctgagagtac tccgccataa
     4561 tgcccgcgac acgcaaataa cacttcacat cagccagact actgccacca aatttatgca
     4621 gttgacgacc tttcgcccct gcctgcgcaa tcacactcat ttttacccct tgtttgcagc
     4681 ccggaagcca ttttccaggt cggcaattaa atcttcgcca tcttcaatac cggtggagat
     4741 acgcagcagc gtctcggaga tcccggcggc agcacgcgct tctggtgcca tgcctgcatg
     4801 tgtcatggtt gcggcgtgag agattaaact ttccactccc cctaatgatt ccgccagcgt
     4861 aaacaacgac agcccaccca ggaaacgacg cagcgtctgc tcatcgccat ccagttcaaa
     4921 actcaacatt gcgccaaagc ctttttgctg gcgcgcggca atttcatgcc cctgattttc
     4981 cggcagcgac gggtgataca gttttttcac caacggctgg gtttgcagat atttcacaat
     5041 cgcctgcgcg ttgcgctggg ccagctccat tcgcggcacc agcgttcgca acccgcgcag
     5101 cagcagatag ctgtcaaacg cgccgcccgt cacgccaata ttgtttgccc accaggcaag
     5161 ttcagtgaca acgtccgggt ctttagcaat cactacgcct gccaccacgt cagagtgacc
     5221 gttcagatat ttcgtgcatg aatgcaacac cagatcggca cctaatgcca gtggattttg
     5281 taatgccggg cttaagaagg tgttatccac cacgctcacc gccccgactt cccttgccag
     5341 atggcagatt ttcgcaatat ccacgacgcg taacaatgga ttacttgggc tttctaccag
     5401 taccagtttg ggtttttctg ccagcgctgc ccgtaatgcc tgttcatcgc tttgatcaac
     5461 aaacaacacg cgatagcaac cgcgtttcgc caggctgtcg aacaggcgat agctaccgcc
     5521 gtagcagtcg tgcggcgcaa ccagcagatc gccaggtttc aaaaagacgg tcgttaccag
     5581 gtgaatcgcg gacatgccgg tattagtaag tactgcacca gcaccgcctt ccagttctgc
     5641 cagcgctcgc tgaaccacat cgcgcgttgg gttgccgcgc cgcgagtaat catgctcgcg
     5701 cggttcatta aatccggtaa agttataggt actggaaaga tggatgggag ggacaacgca
     5761 accatactgt tcgtcgtcat ttaacccgct acgcactgcg atggtggcct gtttacgcgt
     5821 catgtgatga agttccctgg gcttggtcgg tgaaatgtca ggcaccagag taaacattgt
     5881 gttaatggac gtcaatacat ctggacatct aaacttcttt gcgtatagat tgagtaaatc
     5941 tcaaatagcc gttaaaatta tatgcattat cacgccgaca ggtgcattac acgatgtcac
     6001 ggtaacgcct gtacggtaaa ctatgcgggt ttacggtcag tacccatatt aattgtgtgg
     6061 tctggcctca atttattgac gaagaggatt aagtatctca tggctgaatg gagcggcgaa
     6121 tatatcagcc catacgctga gcacggcaag aagagtgaac aagtcaaaaa aattacggtt
     6181 tccattcctc ttaaggtgtt aaaaatcctc accgatgaac gcacgcgtcg tcaggtgaac
     6241 aacctgcgtc acgcgaccaa cagcgagctg ctgtgcgaag cgtttctgca tgcctttacc
     6301 gggcaacctt tgccggatga tgccgacctg cgtaaagagc gcagcgacga aattccggag
     6361 gcggcaaaag agatcatgcg tgagatgggg attaacccgg agacgtggga atactaaagc
     6421 ggaaaagagc ggcgcggagt taaccagccg cttttttcat acatggacgt ttaacgatga
     6481 aaatcaggct actgattctc agcctgctgg tttctgtgcc tgcatttgcc tggcaaccac
     6541 aaaccggcga catcatcttt cagatctctc gctcatcgca aagtaaagcg atccaactgg
     6601 cgacccattc cgattacagc cacaccggta tgctggtgat acgcaacaaa aagccctacg
     6661 tttttgaagc ggtcggcccg gtaaaataca ccccactcaa gcagtggatc gcccatggtg
     6721 aaaagggcaa atacgttgtt cgccgcgttg aaggcggact gagtgttgaa caacagcaaa
     6781 aactggcgca aacggcaaaa cgttatctcg gtaaaccgta tgactccagc ttctcgtgga
     6841 gcgacgatcg ccagtactgt tcggaagtgg tgtggaaggt ctaccagaat gcgctgggaa
     6901 tacgtgtggg cgaacagcag aagctgaaag agttcgatct tagtaacccg ctggttcagg
     6961 caaagctcaa agaacgctac ggcaaaaata ttccgcttga ggaaacggta gtttcaccac
     7021 aagccgtttt cgacgcgcca caactcacca cggtcgccaa agaatggccg ctgttttcct
     7081 ggtaatacga ccaaaattca ggcacaaaaa aagcgccgtg cggcgctttt ttcggaaatc
     7141 cggtcttatt tgctgcccgg gatgttgaaa cgcttgttga agcggtcaac acggccaccg
     7201 gtagcaacat cacgctgttt gccagtgaag aacgggtggc acttgctgca cacgtcgagg
     7261 ttcaggtcat gaccaacggt ggagcggatt ttcattacgt taccgcaaga gcagctagca
     7321 gtaatttctt cgtatttcgg gtgaatatct tttttcatgg gaaaacctcg gtttaaggcc
     7381 gcgtcgctct tccagcccta acgccagaca ccacgcgatg ttaaaagtat agcttcaata
     7441 cgatcatttc gtacgaaggc gcgaaatcat acagaaatta accagcgtat gcaaactgat
     7501 ccgcactctt ctacggcaat gtgtatacta acccaccgaa tttcaagtca ggatgatgct
     7561 atgcccgttg cccacgttgc cttgcccgtt ccgcttcctc gtacctttga ctatctgctg
     7621 ccagaaggca tgacggttaa agccgggtgt cgcgtgcgcg tgccgtttgg caaacagcag
     7681 gagcgcatcg ggattgtggt atcagttagc gatgccagcg aactgccgct caatgagcta
     7741 aaagcggtag tcgaagtgct ggacgttgag ccggtattta ctcactccgt ctggcgatta
     7801 ctgctctggg cggcagatta ctatcatcat ccgattggcg atgtgctgtt tcatgcctta
     7861 ccgattttac tgcgccaggg gcggcctgcg gcgaacgcgc cgatgtggta ctggtttgcc
     7921 actgaacaag gtcaggcggt ggatctgaac agcctgaaac gttcgccaaa gcaacaacag
     7981 gcgctggcag cgttacggca acgcaaaatc tggcgtgatc aggtcgcgga acttgaattt
     8041 aatgatgccg cgttgcaggc gctgcgcaaa aaaggtctgt gtgatttagc aagtgaaaca
     8101 ccagagttta gcgactggcg aaagaattat gccgtttctg gtgagcggtt gcgattgaat
     8161 accgaacagg ccaccgccgt tggcgcaatt catagcgcgg cagatacttt ttctgcctgg
     8221 ctgctggcgg gcgttaccgg ttccggtaaa acggaggttt atctcagcgt actggaaaac
     8281 gtgctcgctc agggcaaaca ggcgctggtg atggtgccgg aaatcggcct gacaccgcaa
     8341 actatcgccc gttttcgtga acgttttaat gcccccgtgg aagttctgca ttccggcctg
     8401 aacgacagcg agcgtctttc ggcgtggttg aaagcgaaaa atggcgaggc ggcgattgtg
     8461 atcggcaccc gctccgcgct gtttacaccg tttaaaaatc tcggcgtgat tgtcattgat
     8521 gaagagcacg acagttccta taaacagcag gaaggctggc gctaccatgc ccgcgacctg
     8581 gcggtgtatc gtgcgcatag cgagcaaatc ccaattattc ttggctctgc aacgcccgca
     8641 ctggaaacgt tatgcaacgt gcagcagaaa aaataccgcc tgctgcgcct gacccgtcgg
     8701 gcgggcaatg cgcgcccggc aattcaacat gtgctggatt taaaaggtca gaaggtgcag
     8761 gcaggtctgg ctccggcgtt aatcacccgt atgcgccagc atttacaggc cgacaaccag
     8821 gttatcctct ttcttaaccg ccggggtttt gcgcctgcgc tactgtgcca cgactgtggt
     8881 tggatagccg aatgcccacg ttgcgatcac tactacacgc tgcatcaggc gcaacagcat
     8941 ttgcgctgcc accattgcga cagtcagcgt ccggtgccgc gccagtgccc ttcttgcggt
     9001 tccacgcacc tggtacccgt ggggctgggc accgaacagc ttgaacagac gctcgcgccg
     9061 ttgttccctg gtgtccccat ttctcgtatc gaccgcgata ccaccagccg caaaggcgcg
     9121 ctggaacaac aactggcgga agtacatcgc ggtggcgcac ggatattgat cggtacacaa
     9181 atgctggcga aaggtcacca ctttccggat gtgacgctgg tggcgttact ggatgtggac
     9241 ggcgcgctgt tttctgcgga ttttcgctcg gcagagcgtt ttgcccagct ttatacccag
     9301 gtggcaggcc gtgccgggcg tgcgggtaaa cagggcgaag tagttctgca aacgcaccat
     9361 cctgaacatc cgctgttgca aacgttgctt tataaaggct acgacgcctt tgccgaacag
     9421 gcactggctg aacgacgcat gatgcagctg ccgccgtgga ccagccatgt gattgtgcgt
     9481 gcggaagatc ataacaatca gcacgcgcca ttgttcctgc aacaactgcg taatctcatt
     9541 ctcgccagcc cactggcaga cgagaaactg tgggttctcg gtccggttcc ggcgctggcc
     9601 cctaaacgtg gcggtcgctg gcgctggcag atattgttgc agcacccttc ccgcgtgcgc
     9661 ttacagcaca tcatcaatgg tacgctggcg cttattaata caataccgga ttcccgcaag
     9721 gtgaaatggg tgctggatgt cgatccgatt gagggttaaa ccgctcacga tgcgaggcgg
     9781 atcgaaaaat tcaatattca tcacactttt catgaaaatt ctgtaaccgt tttcacgcgc
     9841 tatctgctaa aaatgttgcc gatgtgaagt aaacatggat gtagtacgcc tgacgtgcca
     9901 ggcgaggagt gagtgtgaaa gcgaagaagc aggaaactgc cgcgaccatg aaagacgttg
     9961 ccctcaaggc aaaagtctct acagcgaccg tctcccgagc attaatgaat cccgataaag
    10021 tctcccaggc cactcgtaat cgggttgaaa aagcggcccg ggaagtgggt tatttaccac
    10081 agcctatggg gcgcaacgtc aagcgtaatg aatcccgtac cattctggtg attgtcccgg
    10141 atatctgcga tcccttcttt agcgaaatta ttcgcggtat cgaagttacg gcggcaaatc
    10201 acggatatct ggtgctgatt ggcgactgtg cgcatcaaaa tcagcaggaa aaaaccttta
    10261 tcgatttgat catcaccaag caaattgatg gcatgttgtt gctgggttca aggctgccgt
    10321 ttgatgccag cattgaggaa cagcgtaatc tgccgccgat ggtgatggcg aacgaatttg
    10381 caccggaact ggagctgcct accgttcata tcgacaatct gaccgccgca tttgatgcag
    10441 taaattattt atatgagcaa gggcataatc ggattggctg tatagccggt cccgaagaga
    10501 tgccgctgtg tcactaccgc ctgcaaggct atgtccaggc gctgcgtcgc tgcggcatta
    10561 tggttgatcc gcaatacatc gcccgtggcg acttcacctt cgaagccgga agcaaagcga
    10621 tgcagcagtt gcttgatctt ccacaaccgc ctactgctgt cttctgccat agcgatgtga
    10681 tggcgctcgg cgcactttct caggcaaaac gccaggggct gaaagtcccg gaagaccttt
    10741 ccataatcgg ttttgataac atcgacctga cgcaattttg tgatccgccg ctgacaacca
    10801 tcgcgcagcc gcgttacgaa atcggtcggg aagctatgct gttattgctt gatcaaatgc
    10861 aggggcaaca cgttggcagt ggctctcgtt taatggactg cgaacttatc atccggggat
    10921 caacacgcgc gttaccttaa agtaaacctt taagactgcc gtgactggtc aaaggcccgc
    10981 cgcttaagta acatggcggg ctgacgaacg aataaataca gcgaaacgat agtggcacaa
    11041 cgagattatg tacgccgcag ccaaccggca ccttcgcggc gaaaaaagag cacctcacgg
    11101 aaaaagcaac gaaatctgcc tgcggtttct cccgctatgg tcgctattgc tgccgccgtt
    11161 cttgtgacct ttatcggtgg tctgtacttc attacgcatc acaagaaaga agagtccgag
    11221 acgctgcaaa gccagaaagt gaccggaaac ggactaccac caaaaccaga agaacgctgg
    11281 cgctacatta aagagctgga aaatcgccag ccgggtgtgc gtgcgcccac agaaccttct
    11341 gccggtggtg aagtgaaaac gccggagcaa ctgacaccag aacaacgcca gcttcttgaa
    11401 caaatgcagg ccgatatgcg ccagcagcca acgcagctgg ttgaagttcc gtggaatgag
    11461 cagacgccgg aacagcgtca gcaaacgcta cagcgccagc gtcaggcgca gcaattagcg
    11521 gagcagcaac gcctggctca gcagtcgcgt acgactgaac aaagctggca gcagcagacg
    11581 cgtacgtcgc aagccgcacc ggtacaggcg caaccgcgcc agtctaaacc ggcttcttct
    11641 cagcagccgt accaggatct gctgcaaact cctgcgcaca cgactgcgca gtcgaagccg
    11701 cagcaagctg ccccggttgc tcgtgccgct gacgcgccaa aaccgacggc ggagaaaaaa
    11761 gacgaacgcc gctggatggt gcagtgcggt tcgttcagag gcgcggaaca ggcagagacg
    11821 gtacgtgctc agctggcgtt cgaaggcttt gactcgaaaa tcaccaccaa caatggctgg
    11881 aatcgtgtgg tcattggccc ggtgaaaggc aaagagaacg cagacagcac cctcaatcgc
    11941 ttgaagatgg cgggtcatac aaactgcatt cggctcgccg ccgggggttg aaaccctcaa
    12001 aatccccccc atctataatt gcattatgcc ccgtactttt gtacggggtt tgtactctgt
    12061 attcgtaacc aaggggtcag ctcgtgacaa ctatagtaag cgtacgccgt aacggccatg
    12121 tggtcatcgc tggtgatggt caggccacgt tgggcaatac cgtaatgaaa ggcaacgtga
    12181 aaaaggtccg ccgtctgtac aacgacaaag tcatcgcggg ctttgcgggc ggtactgcgg
    12241 atgcttttac gctgttcgaa ctgtttgaac gcaaactgga aatgcatcag ggccatctgg
    12301 tcaaagccgc cgttgagctg gcaaaagact ggcgtaccga tcgcatgctg cgcaaacttg
    12361 aagcactgct ggcagtcgcg gatgaaactg catcgcttat catcaccggt aacggtgacg
    12421 tggtgcagcc agaaaacgat cttattgcta tcggctccgg cggcccttac gcccaggctg
    12481 cggcgcgcgc gctgttagaa aacactgaac ttagcgcccg tgaaattgct gaaaaggcgt
    12541 tggatattgc aggcgacatt tgcatctata ccaaccattt ccacaccatc gaagaattaa
    12601 gctacaaagc gtaaggatct cccatgtctg aaatgacccc acgcgaaatc gtcagcgaac
    12661 tggataagca catcatcggc caggacaacg ccaagcgttc tgtggcgatt gctctgcgta
    12721 accgctggcg tcgcatgcag ctcaacgaag agctgcgcca tgaagtgacc ccgaaaaata
    12781 tcctgatgat cggcccgacc ggtgtcggta aaactgaaat cgcccgtcgt ctggctaagc
    12841 tggcgaatgc gccgttcatc aaagttgaag cgaccaaatt caccgaagtg ggctatgtcg
    12901 gtaaggaagt ggattctatt attcgcgatc tgaccgatgc cgccgtgaaa atggtacgcg
    12961 tccaggctat cgagaaaaac cgttatcgcg ctgaagaact ggcagaagaa cgtattctcg
    13021 acgtgctgat cccacctgct aaaaacaact ggggacagac cgaacagcag caggaaccgt
    13081 ccgctgctcg tcaggcattc cgcaagaaac tgcgtgaagg ccagcttgat gacaaagaaa
    13141 tcgagatcga tcttgccgca gcaccgatgg gcgttgaaat tatggctcct ccgggaatgg
    13201 aagagatgac cagccagctg cagtccatgt tccagaacct gggcggccag aagcaaaaag
    13261 cacgtaagct gaaaatcaaa gacgccatga agctgctgat tgaagaagaa gcggcgaaac
    13321 tggtgaatcc ggaagagctg aagcaagacg ctatcgacgc tgttgagcag cacgggatcg
    13381 tgtttatcga cgaaatcgac aaaatctgta agcgcggcga gtcttccggt ccggatgttt
    13441 ctcgtgaagg tgttcagcgt gacctgctgc cgctggtaga aggttgcacc gtttccacca
    13501 aacacgggat ggtcaaaact gaccacattc tgtttatcgc ttctggcgcg ttccagattg
    13561 cgaaaccgtc tgacctgatc ccggaactgc aaggtcgtct gccaatccgc gttgagctgc
    13621 aggcactgac caccagcgac ttcgagcgta ttctgaccga gccgaatgcc tctatcaccg
    13681 tgcagtacaa agcactgatg gcgactgaag gcgtaaatat cgagtttacc gactccggta
    13741 ttaagcgcat cgcggaagcg gcatggcagg tgaacgaatc taccgaaaac atcggtgctc
    13801 gtcgtttaca taccgttctg gagcgtttaa tggaagagat ttcctacgac gccagcgatt
    13861 taagcggtca aactatcatc attgacgcag attatgtgag caaacatctg gatgcgttgg
    13921 tggcagatga agatctgagc cgttttatcc tataatcgcg ttcaatcatt ttcatcattg
    13981 tttgatgggg ctgaaaggcc ccatttttat tggcgcgtat tatgactgaa caacaaatta
    14041 gccgaactca ggcgtggctg gaaagtttac gacctaaaac cctccccctc gcctttgctg
    14101 caattatcgt cgggacggcg ctggcatggt ggcaaggtca cttcgatccg ctggtcgccc
    14161 tgctggcact gattaccgcc gggctattac agatcctttc taacctcgcc aatgattacg
    14221 gcgatgcggt aaaaggcagc gataaacctg accgtatcgg accgctacgc ggcatgcaaa
    14281 aaggggtcat tacccagcaa gagatgaaac gggcgctcat tattaccgtc gtgctcatct
    14341 gtctctccgg gctggcactg gttgcagtgg cgtgccatac actggccgat tttgtcggct
    14401 tcctgattct tggcgggttg tcgatcattg ccgctatcac ctacaccgtg ggcaatcgtc
    14461 cttatggtta tatcggtctg ggtgatattt ccgtactggt tttctttggc tggttgagcg
    14521 tcatggggag ctggtattta caggctcata cattgattcc ggcactgatc cttccggcga
    14581 ccgcatgcgg cctgctggca acggcagtac tgaacattaa taacctgcgt gatatcaata
    14641 gcgaccgcga aaacggcaaa aacacgctgg tggtgcgctt aggtgcagta aacgcgcgtc
    14701 gttatcatgc ctgcctgctg atgggctcac tggtgtgtct ggcgctgttt aatctctttt
    14761 cgctgcatag cctgtggggc tggctgttcc tgctggcggc accgttgctg gtgaagcaag
    14821 ctcgttatgt gatgcgcgaa atggacccgg tggcgatgcg accaatgctg gaacgcactg
    14881 tcaaaggagc gttactgact aacctgctgt ttgttttagg gatattccta agccagtggg
    14941 cagcataact gacaaatatc aattaacaat tgatgatttt gccaacagcc cacatagcgc
    15001 gatatactga aaattctcgc agcaactgaa tgttaagcct atgaaatacg atacttccga
    15061 gctttgtgac atctatcaag aagatgttaa cgtcgtggaa ccgctgttct ccaactttgg
    15121 cggacgagcg tcgtttggcg gacaaataat cacggtaaaa tgtttcgagg acaacgggtt
    15181 gctgtacgat ctgctcgaac agaatggccg tggtcgtgtt ctggtcgtcg atggcggtgg
    15241 ttctgttcgt cgcgcactgg tcgatgctga actggcgcgt ctggcagtac aaaatgaatg
    15301 ggaaggtctg gtcatttacg gcgcggtgcg tcaggtagat gacctggaag agctggatat
    15361 cggcatccag gcgatggcgg caattccggt tggtgcctct ggcgaaggca ttggcgaaag
    15421 cgatgtccgc gtcaattttg gcggtgtcac cttcttctcc ggcgaccatc tttatgccga
    15481 caataccggg attattcttt cagaagatcc gttggatatt gaatgataga aaaggcaccg
    15541 caaggtgcct tttttctgcg ttacctgttg gcctacacag taaagaaatt acgcggaaga
    15601 tgaagcgtaa tcagacctct tccatgcgac ccagcagggc ctgcagacgt tcctgccagc
    15661 cgttctgctg ttctttcaga tggttgttct cacgctccag ctcttcgcgc tgatgctggg
    15721 cattttgaac ttcctgcgac agtgagttgt ttttttcttt cagctcttcg atttccatct
    15781 gcaacagagt gatggtatca atcgcctgct gtacttttgc ttccagtttc tcaaacactt
    15841 ctaatgacat tgtcatacct ctcctgaatt gcaaggcgtt gatggataaa aatcctcgtc
    15901 ccgattaccg gtgacgcctt aataaatacg agcgcacttt agttagctcc gattgtatga
    15961 agccgcgcca tcgctgtcca gcggcacgcc ttgcagatta cggtttgcca cacttttcat
    16021 ccttctcctg gtgacataat ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat
    16081 gatctaacaa acatgcatca tgtacaatca gatggaataa atggcgcgat aacgctcatt
    16141 ttatgacgaa gcacacacat tttaagttcg atatttctcg tttttgctcg ttaacgatga
    16201 gtttacagca tgcctacaag catcgtggag gtccgtgact ttcacgcata caacaaacat
    16261 taactcttca ggatccgatt atgagtcaaa catcaacctt gaaaggccag tgcattgctg
    16321 aattcctcgg taccgggttg ttgatttttt tcggggtggg ttgcgttgca gcactaaaag
    16381 tcgctggtgc gtcttttggt cagtgggaaa tcagtgtcat ttggggactg ggggtggcaa
    16441 tggccatcta cctgaccgca ggggtttccg gcgcgcatct taaccccgct gttaccattg
    16501 cattgtggct gtttgcctgt ttcgacaagc gcaaagttat tccttttatc gtttcacaag
    16561 ttgccggcgc tttctgcgct gcggctttag tttacgggct ttactacaat ttatttttcg
    16621 acttcgagca gactcatcat attgttcgcg gcagcgttga aagtgttgat ctggctggca
    16681 ctttctctac ttacccgaat cctcatatca attttgtgca ggctttcgca gttgagatgg
    16741 tgattaccgc tattctgatg gggctgatcc tggcgttaac ggacgatggc aacggtgtac
    16801 cacgcggccc tttggctccc ttgctgattg gtctactgat tgcggtcatt ggcgcatcta
    16861 tgggcccatt gacgggtttt gccatgaacc cagcgcgtga cttcggtccg aaagtctttg
    16921 cctggctggc gggctggggc aatgtcgcct ttaccggcgg cagagacatt ccttacttcc
    16981 tggtgccgct ttttggccct atcgttggcg cgattgtagg tgcatttgcc taccgcaaac
    17041 tgattggtcg ccatttgcct tgcgatatct gtgttgtgga agaaaaggag accacaactc
    17101 cttcagaaca aaaagcttcg ctgtaatatg actacgggac aattaaacat gactgaaaaa
    17161 aaatatatcg ttgcgctcga ccagggcacc accagctccc gcgcggtcgt aatggatcac
    17221 gatgccaata tcattagcgt gtcgcagcgc gaatttgagc aaatctaccc aaaaccaggc
    17281 tgggtagaac acgacccaat ggaaatctgg gccacccaaa gctccacgct ggtagaagtg
    17341 ctggcgaaag ccgatatcag ttccgatcaa attgcagcta tcggtattac gaaccagcgt
    17401 gaaaccacta ttgtctggga aaaagaaacc ggcaagccta tctataacgc cattgtctgg
    17461 cagtgccgtc gtaccgcaga aatctgcgag catttaaaac gtgacggttt agaagattat
    17521 atccgcagca ataccggtct ggtgattgac ccgtatttct ctggcaccaa agtgaagtgg
    17581 atcctcgacc atgtggaagg ctctcgcgaa cgtgcgcgtc gtggtgaatt gctgtttggt
    17641 acggttgata cgtggcttat ctggaaaatg actcagggcc gtgtccatgt gaccgattac
    17701 accaacgcct ctcgtaccat gttgttcaac atccataccc tggactggga cgacaaaatg
    17761 ctggaggtgc tggatattcc gcgcgagatg ctgccagaag tgcgtcgttc ttccgaagta
    17821 tacggtcaga ctaacattgg cggcaaaggc ggcacgcgta ttccaatctc cgggatcgcc
    17881 ggtgaccagc aggcggcgct gtttggtcag ttgtgcgtga aagaagggat ggcgaaaaat
    17941 acctacggca ctggctgctt tatgctgatg aacactggcg agaaagcggt gaaatcagaa
    18001 aacggcctgc tgaccaccat cgcctgcggc ccgactggcg aagtgaacta tgcgctggaa
    18061 ggtgcggtgt ttatggcggg cgcgtccatt cagtggctgc gcgatgagat gaagttgatt
    18121 aacgacgcct acgattccga atatttcgcc accaaagtgc aaaacaccaa tggtgtgtat
    18181 gtggttccgg cctttaccgg gctgggtgcg ccatactggg atccgtatgc acgcggggcg
    18241 attttcggtc tgactcgtgg ggtgaacgct aaccacatta tacgcgcgac gctggagtct
    18301 attgcttatc agacgcgtga cgtgctggaa gcaatgcagg ccgactccgg tattcgtctg
    18361 cacgcgctgc gcgtggacgg cggtgcggtg gcgaacaatt tcctgatgca gttccagtcc
    18421 gatattctcg gtacgcgcgt tgagcgcccg gaagtgcgcg aagtcaccgc tttgggtgcg
    18481 gcctatcttg ctggtctggc ggttggcttc tggcagaatc ttgacgagct gcaagagaaa
    18541 gcggtgattg aacgcgagtt ccgtcctggc atcgaaacca ctgagcgtaa ttaccgttac
    18601 gcaggctgga aaaaagcggt caaacgcgcg atggcatggg aagaacacga cgagtaataa
    18661 tgattgcctg atgcgacgct tgcgcgtctt atcaggccta cgagtcggat acacatccgg
    18721 catcacacag actctgaaac cgtgcggggg caaccccgca cacatcaata atcccgccct
    18781 tcccctgtgc tacacttcgc gccattcctt actgcttaga gtttgctatg agacgagaac
    18841 ttgccatcga attttcccgc gtcaccgaat ctgcggcgct ggctggctac aaatggttag
    18901 gacgcggcga taaaaacacc gcggacggcg cggcggtaaa cgccatgcgt attatgctca
    18961 accaggtcaa cattgacggc accatcgtca ttggtgaagg tgaaatcgac gaagcaccga
    19021 tgctctacat tggtgaaaaa gtcggtactg gtcgcggcga cgcggtagat attgctgttg
    19081 atccgattga aggcacgcgc atgacggcga tgggccaggc taacgcgctg gcggtgctgg
    19141 cagtaggcga taaaggctgt ttcctcaacg cgccagatat gtatatggag aagctgatcg
    19201 tcggaccggg agccaaaggc accattgatc tgaacctgcc gctggcggat aacctgcgca
    19261 atgtagcggc ggcgctcggc aaaccgttga gcgaactgac ggtaacgatt ctggctaaac
    19321 cacgccacga tgccgttatc gctgaaatgc agcaactcgg cgtacgcgta tttgctattc
    19381 cggatggcga cgttgcggcc tcaattctca cctgtatgcc agacagcgaa gttgacgtgc
    19441 tgtacggtat tggtggcgcg ccggaaggcg tggtttctgc ggcggtgatc cgcgcattag
    19501 atggtgacat gaacggtcgt ctgctggcgc gtcatgacgt caaaggcgac aacgaagaga
    19561 atcgtcgcat tggcgagcag gagctggcac gctgcaaagc aatgggcatc gaagccggta
    19621 aagtattacg tctgggcgat atggcacgca gcgataacgt catcttttct gccaccggta
    19681 ttaccaaagg cgatctgctg gaaggcatta gccgcaaagg caatatcgcg actaccgaaa
    19741 cgctgctgat ccgcggcaag tcacgcacta ttcgccgcat tcagtccatc cactatctgg
    19801 atcgcaaaga cccggaaatg caggtgcaca ttctctgagt gatttgatcg attgagcctt
    19861 ccagtccttc gggactggaa tttttttgtt cggagaacga agataaggca agtcaatcaa
    19921 aacaggagaa aaacatggct gattgggtaa caggcaaagt cactaaagtg cagaactgga
    19981 ccgacgccct gtttagtctc accgttcacg cccccgtgct tccgtttacc gccgggcaat
    20041 tcaccaagct tggccttgaa attgacggcg aacgcgtcca gcgcgcctac tcctatgtta
    20101 actcgcccga taatcccgat ctggagtttt acctggtcac cgtccccgat ggcaaattaa
    20161 gcccacgatt ggcggcactg aaaccgggcg atgaagtgca ggtggttagc gaagcagctg
    20221 gcttctttgt tctggatgaa gtaccagatt gcgaaacgct atggatgctg gcaaccggta
    20281 cagcgattgg cccttattta tcgattctgc aactgggcaa agatttagat cgcttcaaaa
    20341 atctggtcct ggttcacgcc gcacgttatg ccgccgactt aagctatttg ccactgatgc
    20401 aagaactgga aaaacgctac gaaggcaaac tgcgcattca gacggtggtc agtcgggaaa
    20461 cggcagcggg gtcgctcacc ggacggatac cagcgttaat tgaaagtggt gaactggaaa
    20521 gcgcggttgg cctgccgatg aataaagaaa ccagccatgt gatgctgtgc ggcaatccac
    20581 agatggtgcg cgatacgcaa cagttgctga aagagacccg gcagatgacg aaacatttac
    20641 gtcgccgacc gggccatatg acagcggagc attactggta agcggttact tatcgataaa
    20701 cggcacgatg agcaaatccg cactcaactt attgatcatc ccgcgatatg ccggcatcaa
    20761 acggttgata aatgagtggt gatgaccaca gacaaggagg tcgcactgct ctttttgcat
    20821 aatttccagc agtgtttccg gcatttctcc gcgttcaata cgcagttttg tcttcggcca
    20881 ttgaatattt ttcgtcagtt tatacagctt gttatccgac ttattcttca acaattgaag
    20941 aatatcttct gttgcaggga agtagatacc cggatacaac tcgcttaagc catcatcaat
    21001 atgaattaac gtcaggtgag cgtcattatg tctggcgagc tctagggctt tattcaccag
    21061 taaggcatct tcttcattcc cggaaattgc cacgccaatg tgtttataag ccatatttaa
    21121 ctccttctaa agcaactcca tcaagctaac gaacgcagtg ataactcaaa aataatcatc
    21181 tcggcctggc atgagaaggt gaaggccgca tcaagctcaa cttgcccttc cagatcctta
    21241 atcttatagg ccacgctggc cccttcatct gcagaggatg cagcggcggc aagctctttc
    21301 agtttggtca taaaggactc tgcttcttcg cgggtggcaa aaaagcgcga aaacttactg
    21361 gtacagttgt cgttatcaat caccgtaccg atatcaatcg cacatccttt agtactgcat
    21421 ttatctacga catctttcat agggcacctc tgtatattcg cctctctgtc gattacgact
    21481 taagaataat gcgccgttaa aggaagaaca aacaagtaat gatcttatgg attaccattt
    21541 tttttgatat tgcttaaggt gaagagttca gaaggatttt ccgggcattg tcacttgcat
    21601 cgtggaaata acaatcccgg aaaaaaatag cgataagaga tatattaaag gttaattact
    21661 tgatttattg tcggctttat acttcacatc ctgggtatct ttaccatatt tattttcgcc
    21721 ttgtgtgcca acaaacgcac caagatcgat aagcatcatc accagaatca atgtcgggat
    21781 aaaacgcccc accgcccatt gccagacacc cggtaaaatt gcccagttac ccgccagcag
    21841 catccacgcc acaatcatca gaaatgccca tgcgccggaa cgcccgcgat catgcaagcg
    21901 cttaacggtt actgccgctg ttggccaaag caagcacaca aggcaaaacg ccgcggtctg
    21961 aatatcgagt aaatttttac ccgccagtga gaaaagcacc agcatgcctg cgaaccacag
    22021 gcctatccaa atccagaaat cacggcgtcc aatacgccct ttaaatgaga ataaccattg
    22081 ctgtatggtc atgtaagttc cttgatggtt gtcttttcca ggattctacc cttttgacaa
    22141 gggcgacaga tatcgtttta atcggagcca gtcataacaa aaggtactgt caatgaagcc
    22201 agggtgtacg ctgttttttc tcttatgttc tgcattaacc gttacaacaa cggcgcatgc
    22261 acaaacacca gatacggcaa cgaccgcgcc ttatctgctg gctggagccc ctactttcga
    22321 tctctccatc agccagtttc gagaagactt taacagccag aatcccagcc tgccactgaa
    22381 cgaatttcgt gccatcgaca gcagtcccga caaagccaat ctcactcgtg cagcaagtaa
    22441 aattaacgag aacttgtatg cttctactgc gctggagcgc ggtaccttaa aaatcaaaag
    22501 cattcaaata acctggctac ccatccaggg gccagagcaa aaagccgcga aagcgaaagc
    22561 tcaggcatac atggcagcgg tgatccgcac actcacccca ttaatgacca aaactcaaag
    22621 ccagaaaaaa ctgcagtcgc tactaacggc ggggaaaaac aaacgttatt acaccgagac
    22681 agaaggtgca ctgcgttatg ttgtcgcgga caacggcgaa aaggggctga ccttcgctgt
    22741 tgaaccgatt aagctggcgc tatctgaatc gcttgaaggt ttgaataaat gacaaaaagc
    22801 aaagcctttg tgccgatgaa tctctatact gtttcacaga cctgctgccc tgcggggcgg
    22861 ccatcttcct ttattcgctt ataagcgtgg agaattaaaa tgcgacatcc tttagtgatg
    22921 ggtaactgga aactgaacgg cagccgccac atggttcacg agctggtatc taacctgcgt
    22981 aaagagctgg caggtgttgc tggctgtgcg gttgcaatcg caccaccgga aatgtacatc
    23041 gatatggcga agcgtgaagc tgaaggcagc cacatcatgc tgggtgcgca aaacgtggac
    23101 ctgaacctgt ccggcgcatt caccggtgaa acctctgctg ctatgctgaa agacatcggc
    23161 gcacagtaca tcatcatcgg ccactctgaa cgtcgtactt accacaaaga atctgacgaa
    23221 ctgatcgcga aaaaattcgc ggtgctgaaa gagcagggac tgactccggt tctgtgcatc
    23281 ggtgaaaccg aagcggaaaa cgaagcgggc aaaactgaag aagtttgtgc acgtcagatc
    23341 gacgcggtac tgaaaactca gggtgctgcg gcattcgaag gtgcggttat cgcttacgaa
    23401 cctgtatggg caatcggtac tggcaaatct gcaactccgg ctcaggcaca ggctgttcac
    23461 aaattcatcc gtgaccacat tgctaaagtt gacgctaaca tcgctgaaca agtgatcatt
    23521 cagtacggcg gctctgtaaa cgcgtctaac gctgcagaac tgtttgctca gccagacatc
    23581 gacggcgcgc tggttggcgg tgcttctctg aaagctgatg ctttcgccgt gatcgttaaa
    23641 gctgcagaag cggctaaaca ggcttaagtc tgacaggtgc cggatttcat atccggcact
    23701 tactttccta aactcctcgc cttaacgcaa aatctcacac tgatgatcct ggatttcttc
    23761 ggctgaagca cggttcagcg tcagtagatt tcgttgtgtc gccagcaata caaatgagtt
    23821 atcactctgc cgtaccatcg ccagcccgta gcttcccata tgttcccgcg cctcaggcac
    23881 ttcttccgcc agcatcataa atgggctgcg ttgtaccagt tcgctttccg ttacccgacg
    23941 cgccaggtac tcatgcccac gcaaaccacc tggcagtggc aaccagcggc tactgatgtt
    24001 cgccagattg ttatccagct gttcgcgcac atcaggacga atacaagaaa tatgaatatg
    24061 aaaatggttt tgcgtacgcc cggtgcggga gttgatcgcc agagataccg cgcgatcggg
    24121 aacaggctgg ccgtattttt ggctcataaa atcacgcgcc tgccaggcta accagaaaaa
    24181 attaggcgtt gacggatcgg tcaacaatgg gctttcagta ccgttaatac ggtacgttgg
    24241 catcaacaga tattgcagtg ggccatgacg atcttttaaa accacgtatc cggcgttggg
    24301 tttgacttcc gcacatggcg acgggttttg gttttcctgc tggttgggca aacattgctc
    24361 aagaacaatc ttacgtaacg tgtctgactc ttcaccggtt aatttccagt aaccaatacc
    24421 ggcagccaca acggcgataa ctatcatcac caaaaaaaga agacccgctt ttttcatctt
    24481 tttttccctg tacctcaaag agatgcaagg gtaacgcaaa atcgtgacaa ataaaaaacc
    24541 cagtcgcgag aaaccaccgg gcttgataat tatcctgggg aaatcagcgt ttgctgatct
    24601 gatcgaacgt accgccgtta gcaaaatgct ctttttgcgc tttcgtccag ccgccgaact
    24661 cttcatcaat ggtgaataac ttcaattttg gaaacgcatt ttcgtacttt ttcgctacct
    24721 cagcgtcgcg cggacggtag tagtttttcg ctgcaatttc ctgaccttct ggcgagtaga
    24781 gatatttcag gtaggcttcc gccacctctt tggtgccttt tttctcgacc actttatcga
    24841 ccaccgacac ggttggctct gcgaggatag actcactcgg cgtgacgatt tcgaatttat
    24901 ctttccccag ttcattcgct gccagcagag cttcattttc ccaggcaatc agtacatcgc
    24961 caattccgcg ctcgacaaaa gtgttagtgg atccgcgcgc gccagaatcc agaacttcga
    25021 cgtttttata cagtgcccga acaaaatcct gtgcttttgc ctgatcgttg ttgttgtgat
    25081 gcagcgcgta gccccaggct gccaggtaat tccagcgcgc gccaccagag cttttcggat
    25141 taggcgtgat caccgaaaca cccggtttaa tcagatcgtt ccagtcatgg atctgcttcg
    25201 gattaccctt acgtaccagg aaaacaatgg tggaagtgta cggtgcggag ttatccggca
    25261 gacgtttgat ccactcttta tcaatccgcc cgcgttcagc aatcgcgtcc acgtcatagg
    25321 ccagagccag cgtgacaaca tcagcttcaa taccgttgat taccgacgtc gcttgtttgc
    25381 ctgaaccacc gtgtgactga cgaatcacca cgttatcgcc agtttgctgt ttccagtggg
    25441 cgctgaatgc cttgttgtac tgttcgtaca attcgcgcgt tggatcatat gaaacgttaa
    25501 gaagctgaat atcctttgcc ataacgctgg ttgccgccag caaaaatgtt aaccctacgc
    25561 cccacttgtt catcgcccga ctctcttatg ttgtgttgtg atgagcaaag cgtgccagaa
    25621 ggttaaccaa acattaaaga ataaaaaaag attggctata acttgcgggt atatgttggg
    25681 ggattaaaaa ggcggaagaa atccgcctca tattgctgac aaagtgcgct ttgtccatgc
    25741 cggatgcggc gcgaacgcct tatccggcct acaaaagttt gcaaattcaa tagattacag
    25801 aattcatgta ggcctgataa gcgaagcgca tcaggcattt ttgcttctgt catcggtttc
    25861 aggctaaagg aatctgcctt tttccgaaat cattaataca gttttttcgc gcagtccagc
    25921 cagtcgcctt tgaacggacg cttcatattt tcgatagcgt cgatgatgtc gtggtgaacc
    25981 agctgttcgt tctggatacc tacgcaacga ccgccgtaac ctgccagcag cagatcgata
    26041 gcgtaagcgc ccatacggga agccagaata cggtcgtaag gcaccggaga accaccgcgc
    26101 tggatgtggc ccagcacagt tgcgcgggtt tcacgaccgg tttctttctc gatgaaatgc
    26161 gccagttcgt caacatcaca catatgttcg gtaatcgcca cgatcgcgtg ttttttacct
    26221 ttcgcgatac ccgctttgat ttcgtttacc agatcttcac ggctgaattc aacttccgga
    26281 accacaacga attcacagcc accggcaatg gccgcagcca acgtcagatc gccacaataa
    26341 cggcccatca cttccaccac ggaaatacgc tggtgagaag aagaggtgtc acgcagacgg
    26401 tcgatcgctt ctacaacggt gctcagcgca gtgaagaaac cgatagtgta gtcagtgcct
    26461 ttgatgtcgt tgtcgatagt gcccggcaga ccgatgcacg ggaagcccat ttcggtcaga
    26521 cgcattgcac ccatgtagga accgtcaccg ccgataacca ccagcgcgtc gataccacgt
    26581 tttttcaggt tttcgatagc cacggcgcgg atgttctcgt cgcggaattc cgggaaacgc
    26641 gcagaaccga ggaacgtacc accacggttg atcatgtcag acacgctgta acggtctagc
    26701 tgtaccatac ggtcttcata cagacccaga tagccgtcat aaatgcccat tacttccaga
    26761 ccttctgtca gcgcagaacg aacaaccccg cgaattgcgg cgttcatgcc tggcgcatca
    26821 ccgccgcttg tcaacacacc gattttctta atcatgacta cctctgaact ttggaatgca
    26881 aaatgaaatc tgttgccgga agtcttcttg cacatcgaag tgatccaacg aatgtgcaaa
    26941 tagtataaca atcacttcct gctgaattga ttcaggtcag gccaaatggc ggtattttat
    27001 acacaaaatg cgggtctggc tctcttttat actgattatg aaagcataga ccgtttaccc
    27061 tccctgggta cgacggaaca ggggtcctga tggataatca catccgatcc cggaaaacgc
    27121 cgtaaaatag cctgctctac ctgatccgcc accatatgtg cctgaaccaa aggcagagag
    27181 tcttccattt ccaaatgaat ctgaataaag cgggtcggcc ctgactgccg cgtgcgaaga
    27241 tcgtgagcgc cgctaacacc cggccaggaa gtcacgatat caataatttc ttgccgttcc
    27301 tcatcaggca atgcgcgatc cagtaatgac tgtaccgcct catatcccat gcgtaacgcg
    27361 ctatataaaa tatagatgcc gatccccaat gcaaacagag catcggcgcg atgccagccg
    27421 taccaggaca accccagcgc cagcagaatt gcgccgttca tcataacatc agactggtaa
    27481 tgtagcatat cagcccgcac cgcctggctt tgcgtccggc gcaccaccca acgctgaaac
    27541 gagacaagga taatcgtaca aattagcgcc acaattgtca cgataacccc gacgcctgga
    27601 tctgtcatcg gtgttggaga taccagatgt tgaatacccg tcaaaaacag gaatagtgcc
    27661 gaaccggaga taaacatact ttgcgccagc gccgcgaggg actcagcttt accgtgacca
    27721 aacgagtgat tatcgtcggc aggttgcagg gaatatcgca ccaccagtaa attcgtcaac
    27781 gacgcgccga tatccaccag cgaatccacc agcgcggcga gaatactcac cgacccggta
    27841 taccaccatg caaaaatttt aatcagcaat agcagcgaag ccatcgccgt cgcagcaatc
    27901 gccgcccgac tgaccagccg tccataagat tgattcataa atactcccgc tatcaactga
    27961 cggtagtata acggaagcaa atcatctgca atgcattaag cagcaggcaa attgaggata
    28021 aaaaaaaccc ccacatcatg tgggggaaga cagggatggt gtctatggca aggaaaacag
    28081 ggtttactac tgggaacgtg agttgctact actcaatagc ttcaacgatg aacttttttg
    28141 ccattgcgtc acgtcgcgca actgctccat tcgttgttga tgtttctcgt ttaaaaccgc
    28201 ttgctgctcc ggcgttaaca ggcgatacat ttggttgcgg acttttgcta tctcaacctg
    28261 acgagctatt tgctcgttcg ccattttttc tgcctgtgcg cgcacagcgt tttcatcaaa
    28321 attttctgcg gtgacaaggc gatgcattgt ctccagttcg ctaacattaa caggaggctg
    28381 ttcgtgccgg gcctgttgca taagatctcg catctgctga cgctgatgtt cggttaaact
    28441 tatgccgtcg aacatatggc tctgcgtact gcgctgcgta agttcttcac ccggatgcca
    28501 gttatcgcct gaaccgactt cagcagcgtg gcttaatgaa ctgactgcca gcgttgaggc
    28561 catgacggca gcggtaacta tgcgcatcat ttgctcccaa aatctttctg tcgcgattca
    28621 acgatagaga gtttacgatt caggctgcaa acatgcgtca gggggtgtaa aacaacgtaa
    28681 agtcatggat tagcgacgtc tgatgacgta atttctgcct cggaggtatt taaacaatga
    28741 ataaaatcct gttagttgat gatgaccgag agctgacttc cctattaaag gagctgctcg
    28801 agatggaagg cttcaacgtg attgttgccc acgatgggga acaggcgctt gatcttctgg
    28861 acgacagcat tgatttactt ttgcttgatg taatgatgcc gaagaaaaat ggtatcgaca
    28921 cattaaaagc acttcgccag acacaccaga cgcctgtcat tatgttgacg gcgcgcggca
    28981 gtgaacttga tcgcgttctc gggcttgagc tgggcgcaga tgactatctc ccaaaaccgt
    29041 ttaatgatcg tgagctggtg gcacgtattc gcgcgatcct gcgccgttcg cactggagcg
    29101 aacaacagca aaacaacgac aacggttcac cgacactgga agttgatgcc ttaagtctca
    29161 atcctggtcg ccaggaagcc agcttcgacg ggcaaacgct ggagttaacc ggtactgagt
    29221 ttaccctgct ctatttgctg gcacagcatc tgggtcaggt ggtttcccgt gagcatttaa
    29281 gccaggaagt gctgggcaag cgcctgacgc ctttcgaccg cgctatcgat atgcacattt
    29341 ccaacctgcg tcgtaaactg ccggatcgta aagatggtca cccgtggttt aaaaccctgc
    29401 gtggtcgcgg ctatctgatg gtttctgctt catgataggc agcttaaccg cgcgcatctt
    29461 cgccatcttc tggctgacgc tggcgctggt gttgatgttg gttttgatgt tacccaagct
    29521 cgattcacgc cagatgaccg agcttctgga tagcgaacag cgtcagggtc tgatgattga
    29581 gcagcatgtc gaagcggagc tggcgaacga tccgcccaac gatttaatgt ggtggcggcg
    29641 tctgttccgg gcgattgata agtgggcacc gccaggacag cgtttgttat tggtgaccac
    29701 cgaaggccgc gtgatcggcg ctgaacgcag cgaaatgcag atcattcgta actttattgg
    29761 tcaggccgat aacgccgatc atccgcagaa gaaaaagtat ggccgcgtgg aactggtcgg
    29821 tccgttctcc gtgcgtgatg gcgaagataa ttaccaactt tatctgattc gtccggccag
    29881 cagttctcaa tccgatttca tcaacttact gtttgaccgc ccgctattac tgctgattgt
    29941 caccatgctg gtcagtacgc cgctgctgtt gtggttggcc tggagtctgg caaaaccggc
    30001 gcgtaagctg aaaaacgctg ccgatgaagt tgcccaggga aacttacgcc agcacccgga
    30061 actggaagcg gggccacagg aattccttgc cgccggtgcc agttttaacc agatggtcac
    30121 cgcgctggag cgcatgatga cctctcagca gcgtctactt tctgatatct ctcacgaact
    30181 gcgcaccccg ctgacgcgtc tgcaactggg tacggcgtta ctgcgccgtc gtagcggtga
    30241 aagcaaggaa ctggagcgta ttgaaactga agcgcaacgt ctggacagca tgatcaacga
    30301 tctgttggtg atgtcacgta atcagcagaa aaatgcgctg gttagcgaga ccatcaaagc
    30361 caaccagttg tggagtgaag tgctggataa cgcggcgttc gaagccgagc aaatgggcaa
    30421 gtcgttgacg gttaacttcc cacctgggcc gtggccgctg tacggcaacc cgaacgccct
    30481 ggaaagtgcg ctggaaaaca ttgttcgtaa tgctctgcgt tattcccata cgaagattga
    30541 agtgggcttt gcggtagata aagacggtat caccattacg gtggacgacg atggtcctgg
    30601 cgttagcccg gaagatcgcg aacagatttt ccgtccgttc tatcggaccg acgaagcacg
    30661 cgatcgtgaa tctggcggta caggtttggg gctggcgatt gttgaaaccg ccattcagca
    30721 gcatcgtggc tgggtgaagg cagaagacag cccgctgggc ggtttacggc tggtgatttg
    30781 gttgccgctg tataagcggt cataacgtca acattcaggt aaaaaaatgc ctgatgcact
    30841 acgcttatca ggcctacaaa acctgttgaa tttatgggtt ttgtaggcag gataaggcgt
    30901 ttacgccgca tccggcattt gagcaggatg cctgatgcga cgctgatagc gtcttatcag
    30961 gcctacactc cggggttatt tttcccacaa tcggcgggaa aaatcttcga tcttgccgct
    31021 aagtcggcgc ttttgcatcg ttcgcgtcca gcttttcgat aaccctgcgg cggagagtaa
    31081 acggtgatac tgatcgtcat caaacggcat atgccatgcg atggcggcag cctcctgcac
    31141 ggtcacatcg ctgacacggg aaaccaactc cagtggcgca tcagcagata ccaaccctgg
    31201 tgcaattacg ctatacaacc accccacttt acccgtgttt tgcatcaact gcgctatatc
    31261 gctgatatca aaatggtaat tgagtttata gcaaggtgag cgtggctggc tgacctgaat
    31321 taatgcctct ccccagcgga aaatatcacc aatataaaca ttactttccg tcagaccgtc
    31381 ggttgagaga ttttcgccaa acgcaggcgc aacaaacagc tccgcctgtt ccggaaattc
    31441 ccgcgcccag tagagataat gctcacgagg ataatgacac agcgctctgt ctggcccgcc
    31501 gtggatcttt ttctccgcct gttcgtcacc ttccagcccc aggtccgtca gcatcaactc
    31561 accatcaacc tggattttgg caattgcgct gggtttgcct tcgggataag cctgaatctt
    31621 gcctgtgtat acatcaaccg gatatcgcat ctgttccctc tccacgagaa attttcccat
    31681 tagaccatat ttattttatg tcaggcgtgc tgttttcgcg gtaagtatcc aggcagaaac
    31741 gcaattgaaa accggacgaa cgttagttca tccggctgtg gggattaagg aagcaggttt
    31801 attttaccgt gcctaaaatt tcgattttcg ccactctggc tttgacttta cgtgaccaga
    31861 tagaggtaag aattggcacc agaatcgaag tcacaatgac cgccgtcgcg accagcgaag
    31921 ttgctgccgg agccatcggt ttaaacgcag gcaccatttc agcaatcagc acaggtgtcg
    31981 ctaccgcggc ccctgcggaa ctggaagcag caattccggc agtgccatcg ccaccgccaa
    32041 tcaatttatc ggcgataatc aacggaatac cggtcacgat aattactgcc acacccaaca
    32101 ggatccccag caaacctgtc tgggcaatta cagtcaaatc aatggtattg cccagtgcga
    32161 aggcaaagaa tggaatcagc gtctgcaccg ctttgctgaa aaattctcgt aactcagggt
    32221 caaggttccc cagggcaaag cccaccagga acggcaatac tgcgccgacg aaaacatgcg
    32281 gttcaaacga ggcaatcccg gcagtgccca gaataatcat cgtcatgagc ggcccggact
    32341 ccaacgacat caacacaaat gccccagctt cttcttttgt gccgtactgc tgcatgatgg
    32401 aagcgtaaag tccgccgttg gtcatatcca tcgccgccac cagcgccaac gttgaaagtc
    32461 cggcaaaaaa tccaacttca acaccatgtt ccggaataat gcgcgaggca attgccgcaa
    32521 ccacccacgc gacggcaatt ttagttacca ccagcgtacc ggatttacgc agtaccgttc
    32581 ccgtcgcgct taattttatt gacgccccca tgcaaaaaaa ccacaccgcc agaatgggca
    32641 ccgtaccggt aatcatcccg ttggtgaatg atccaaaata tttccccgcc cccggcgaga
    32701 aggtgtggca cagtgcgcca aggaatagcg ggacgagcat catccccccc gggattttct
    32761 caatcgagcg ttttatctgc atttccatca cctgtcggca tatttttaag ggctgatgcg
    32821 tcattcctca acggagcatc agtgttgatg gaaacgaaga tagacattca tttgatcaat
    32881 aaaagtgatc aaaaccacac caattgaaac gctgttttat ttttatcgga tcattgtttt
    32941 tattttgaaa ttgatcacaa aaaaaccacc gccgttggcg atggttcatt ttttaagctg
    33001 caatgcggcg taaacgcctc attgcagcag gcggcaaatg attatttttt cgccgcgaaa
    33061 cgtgccgctg cttcgtccca gttcaccacg ttccagaact ctttaatgta gtccggacgg
    33121 cggttctgga atttcagata gtaagcatgt tcccacacat ccaggcccag aatcgggaag
    33181 ccggaagcgc cagaaatggc ttcgcccatc agtggagagt cctggttagc agtagaaacc
    33241 accgccagtt tatcgccttt ttgcaccagc catgcccagc cggaaccaaa gcgggaagct
    33301 gccgcttttt caaattctgc tttgaaatta tcaacggaac cgaaatcacg ttcgatagcc
    33361 gctttcaggt cgccctgcag ggtggtgcct tttttcagac ctttccagaa cagactgtgg
    33421 ttagcgtgac caccagcgtt attgcgcagt acggttttct tgtctgctgg cagctggtcc
    33481 agtttggtga tcagctcttc aaccggcagg ttggcaaatt ctggcaggtt ttccagcgcc
    33541 gcgttggcgt tgttgacgta agtctgatgg tgtttggtgt ggtggatttc catggtctgc
    33601 ttatcgaagt gcggttccag ggcatcgtaa gcatacggca gggatggcag ggtataactc
    33661 atattcatct ccagtgttgt cgggcggccg attgttaatg ccgcgtaagc agttggttca
    33721 ttatagttaa ttaaatgata ttgaaaatga ttatcaatgc cgtacttttc ataagggtat
    33781 ggttttgcag gaaaatgccc aagatgtgaa gcaaatcacc cacttaatgc cgtgattgcc
    33841 agtaaatcgg cagcggcggc aacaggcgaa aggttaatcg acagctcaat ttttacactc
    33901 atctcgtcgg agatgtgacg cgacgaaaaa atgatgagga taagaagatg agtaacgcga
    33961 ttacgatggg gatattttgg catttgatcg gcgcggccag tgcagcctgt ttttacgctc
    34021 cgttcaaaaa agtaaaaaaa tggtcatggg aaaccatgtg gtcagtcggt gggattgttt
    34081 cgtggattat cctgccgtgg gccatcagcg ccctgttact accgaatttc tgggcgtatt
    34141 acagctcgtt tagtctctct acgctgctgc ctgtttttct gttcggcgct atgtggggga
    34201 tcggtaatat caactacggc ctgaccatgc gttatctcgg catgtcgatg ggaattggca
    34261 tcgccattgg cattacgttg attgtcggta cgctgatgac gccaattatc aacggcaatt
    34321 tcgatgtgct gattaatacc gaaggcggac gcatgacgtt gctcggcgtt ctggtggcgc
    34381 tgattggcgt agggattgta actcgcgccg ggcagttgaa agagcgcaag atgggcatta
    34441 aagccgaaga gttcaatctg aaaaaagggc tggtgctggc ggtgatgtgc ggcattttct
    34501 ctgccgggat gtcctttgcg atgaacgccg caaaaccgat gcatgaagcc gctgccgcgc
    34561 tgggcgtcga tcccctgtat gtcgctctgc caagctatgt tgtcatcatg ggcagcggcg
    34621 caatcatcaa cctcggcttc tgcttcattc gtctggcaaa agtgaaagat ttgtcgctaa
    34681 aagccgactt ctcgctggca aaaccgctga tcattcacaa tgtgttactc tcagcactgg
    34741 gcgggttgat gtggtatctg caattctttt tctatgcctg gggccacgcc cgcattccgg
    34801 cgcagtatga ctacatcagc tggatgctgc atatgagctt ctatgtattg tgcggcggta
    34861 tcgtcgggct ggtgctgaaa gagtggaaca atgcaggacg ccgtccggta acggtgttga
    34921 gcctcggttg tgtggtgatt attgtcgccg ctaacatcgt cggcatcggc atggcgaatt
    34981 aatctttctg cgaattgaga tgacgccact ggctgggcgt catcccggtt tcccgggtaa
    35041 acaccaccga aaaatagtta ctatcttcaa agccacattc ggtcgaaata tcactgatta
    35101 acaggcggct atgctggagg agatattgcg catgacacac tctgacctgt cgcagatatt
    35161 gattgatggt cattccagtc tgctgacgaa attgctgacg caaaacgcgc tcactgcacg
    35221 atgcctcatc acaaaattta tccagcgcaa agggactttt caggctagcc gccagccggg
    35281 taatcagctt atccagcaac gtttcgctgg atgttggcgg caacgaatca ctggtgtaac
    35341 gatggcgatt cagcaacatc accaactgcc cgaacagcaa ctcagccatt tcgttagcaa
    35401 acgacacatg ctgactactt tcatgctcaa gctgaccgat aacctgccgc gcctgcgcca
    35461 tccccacgct acctaagcgc cagtgtggtt gccctgcgct ggcgctaaat cccggaatcg
    35521 ctccctgcca gtcaagattc agcttcagcc gctccgggca ataaataata ttctgcaaaa
    35581 ccagatcgtt aacggaagcg taggagtgtt tatcgtcagc atgaatataa aagagatcgc
    35641 cacgggtaat gcgataaggg cgatcgttga gtacatgcag accattacca cgccagacaa
    35701 tcaccagctc acaaaaatca tgtgtatgtt cggcaaagac atcttgcgga taacggtcag
    35761 ccacagcgac tgcctgctgg tcgctggcaa aaaaatcatc tttgagaagt tttaactgat
    35821 gcgccaccgt ggctacctcg gccagagaac gaagttgatt attcgcaata tggcgtacaa
    35881 atacgttgag aagattcgcg ttattgcaga aaaccatccc gtccctgacg aatatcacgc
    35941 ggtgaccagt taaactctcg gcgaaaaagc gtcgaaaagt ggttactgtc gctgaatccg
    36001 cagcgatagg cgatgtcagt aacgctggcc tcgctgtggc gtagcagatg tcgggctttc
    36061 atcagtcgca ggcggttcag gtaacgctga ggcgtcagcc ccgtttgctg cttaagctgg
    36121 cgatgcagcg tacgcaatga aagagaaaat tgatccgcca cagcgtccca attcacctca
    36181 tcggcaaaat ggtcctccag ccaggccaga agcaagttga gacgtgatgc gctgttttcc
    36241 aggttctcct gcaaactgct tttacgcagc aagagcaata attgcataaa taagatctcg
    36301 cgactggcgg tcgagggtaa atcattttcc ccttcctgct gttccatctg tgcaaccagc
    36361 tgtcgcacct gctgcaatac gctgtggtta acgcgccagt gagacggata ctgcccatcc
    36421 tgctcttgtg gcagcaactg attcagcccg gcgagaaact gaaatcgatc cggcgagcga
    36481 tacagcacat tggtcagaca cagattatcg gtatgttcat acagatgccg atcatgatcg
    36541 cgtacgaaac agaccgtccc accggtgatg gtatagggct gcccattaaa tacatgaata
    36601 cccgtgccat gttcgacaat cacaatttca tgaaaatcat gatgatgttc aggaaaatcc
    36661 gcctgcggga gccggggttc tatcgccacg gacgcgttac cagacggaaa aaaatccaca
    36721 ctatgtaata cggtcatact ggcctcctga tgtcgtcaac acggcgaaat agtaatcacg
    36781 aggtcaggtt cttaccttaa attttcgacg gaaaaccacg taaaaaacgt cgatttttca
    36841 agatacagcg tgaattttca ggaaatgcgg tgagcatcac atcaccacaa tgaagcaaat
    36901 tgtgaacatc atcacgttca tctttccctg gttgccaatg gcccattttc ctgtcagtaa
    36961 cgagaaggtc acgaattcag gcgcttttta gactggtcgt aatgaaattc agcaggatca
    37021 cattatgacc tttcgcaatt gtgtcgccgt cgatctcggc gcatccagtg ggcgcgtgat
    37081 gctggcgcgt tacgagcgtg aatgccgcag cctgacgctg cgcgaaatcc atcgttttaa
    37141 caatggcctg catagccaga acggttatgt cacctggaat gtggatagcc tggaaagtgc
    37201 cattcgcctt ggattaaaca aggtgtgcga ggaagggatt cgtatcgata gcattgggat
    37261 tgatacctgg ggcgtggact ttgtgctgct cgaccagcac ggtcagcgcg tgggtctacc
    37321 cgttgcttac cgcgatagcc gcacccatgg cctaatggcg caggcacaac agcaactagg
    37381 caaacgcgat atttatcaac gtagcggcat ccagtttctg cccttcaata cgatctatca
    37441 gctacgtgca ctgacagagc agcaaccaga acttattcca cacattgctc acgctttgct
    37501 gataccggat tactttagtt atcgcctgac cggcaagatg aactgggaat ataccaacgc
    37561 cacgaccacg caactggtca atatcaatag cgacgactgg gacgagtcgc tactggcgtg
    37621 gagcggtgcc aacaaagcct ggtttggtcg cccgacgcat ccgggtaatg tcataggtca
    37681 ctggatttgc ccgcagggta atgagattcc ggtggtcgcc gttgccagcc atgataccgc
    37741 cagcgcggtt atcgcctcgc cgttaaacgg ttcacgcgcc gcttatctct cttctggcac
    37801 ctggtcattg atgggcttcg aaagccagac gccatttacc aatgacacgg cgctggcagc
    37861 caatatcacc aacgaaggcg gggcggaagg tcgctatcgg gtgctgaaaa atattatggg
    37921 cttatggctg cttcagcgag tgcttcagga gcggcaaatc aacgatcttc cggcgcttat
    37981 cgccgcaaca caggcacttc cggcctgccg cttcatcatc aatcccaatg acgatcgctt
    38041 tattaatcct gacgagatgt gcagcgaaat tcaggctgcg tgtcgggaaa cggcgcaacc
    38101 gatcccggaa agtgatgctg aactggcgcg ctgtattttc gacagtctgg cgctgctgta
    38161 tgccgatgtg ttgcatgagc tggcgcagct gcgtggtgaa gatttctcgc aactgcatat
    38221 tgtcggcggc ggctgccaga acacgctgct caaccagctg tgtgccgatg cctgcggtat
    38281 tcgggtgatc gcagggcctg ttgaagcctc gacgctcggc aatatcggca tccagttaat
    38341 gacgctggat gaactcaaca atgtggatga tttccgccag gtcgtcagca ctaccgcgaa
    38401 tctgaccacc tttaccccta atcctgacag tgaaattgcc cactatgtgg cgcagattca
    38461 ctctacacga cagacaaagg agctttgcgc atgaccactc aactggaaca ggcctgggag
    38521 ctggcgaaac agcgtttcgc ggcagtaggg attgatgtcg aggaggcact gcgccaactt
    38581 gatcgtttac ccgtttcaat gcactgctgg cagggcgatg atgtttccgg ttttgaaaac
    38641 ccggaaggtt tgctgaccgg tgggattcag gctactggta attatccggg caaagcgcgt
    38701 aatgccagtg agctacgtgc cgatctggaa caggctatgc ggctgattcc agggccgaaa
    38761 cggcttaatt tacatgccat ctatctggaa tcagacacgc cagtctcgcg cgaccagatc
    38821 acaccagagc acttcaaaaa ctgggttgaa tgggcgaaag ccaatcagct cggtctggat
    38881 tttaacccct cctgtttttc gcatccgcta agcgccgatg gctttacgct ttcccatgcc
    38941 gacgaccgca ttcgccagtt ctggattgac cactgtaaag ccagccgccg tgtttcagcc
    39001 tattttggtg agcaactcgg cacaccatcg gtgatgaaca tctggatccc ggatggcatg
    39061 aaagatatta ccgttgaccg tctcgcccca cgccagcgtt tgctggcagc actggatgag
    39121 gtgatcagcg agaagctaaa ccctgcgcac catatcgacg ccgttgagag caaattgttt
    39181 ggcattggcg cggagagcta cacggttggc tccaatgagt tttacctggg gtatgccacc
    39241 agccgccaga cagcgctgtg cctggacgcc gggcatttcc acccgactga agtgatttcc
    39301 gacaagattt ccgccgccat gctgtatgtg ccgcagttgc tgctgcacgt cagccgtccg
    39361 gttcgctggg acagcgatca cgtagtgctg ctggatgatg aaacccaggc gattgccagt
    39421 gagattgttc gtcacgatct gttcgaccgg gtgcatatcg gcctcgactt cttcgatgcc
    39481 tctatcaacc gcattgccgc gtgggtcatt ggtacacgca atatgaaaaa agccctgcta
    39541 cgtgcgttgc tggaacctac cgctgaacta cgcaagctgg aagcggcggg cgattacact
    39601 gcacgtctgg cgctactgga agagcagaaa tcgttgccgt ggcaggcggt atgggaaatg
    39661 tattgtcagc gtcacgatac gccagcgggt agcgaatggc tggagaacgt gcggacttat
    39721 gagaaagaaa ttttgagtcg ccgcgggtaa acactgccgg atgcggcaca agtgccttat
    39781 ccggcctacg ggtcggcaat agttgtaggc ctgataagac gcgacagcgt cgcatcaggc
    39841 attgattgcc ggatgcggca cgagtgcctt atccggccta cagatcgtac atccggcaac
    39901 aggcacgaac gtccaattaa caggaacaca gaacatgcaa aacataaccc agtcctggtt
    39961 tgtccaggga atgatcaaag ccaccaccga tgcctggctg aaaggctggg acgagcgcaa
    40021 cggcggcaac ctgacgctgc gcctggatga cgccgatatc acaccatatc acgacaattt
    40081 tcaccaacaa ccgcgctata tcccgctcag ccagcccatg cctttactgg caaatacacc
    40141 gtttattgtc accggctccg gcaaattctt ccgtaacgtc cagcttgatc ctgcggctaa
    40201 cttaggcatc gtaaaagtcg acagcgacgg cgcgggctac cacattcttt gggggttaac
    40261 caacgaagcc gtccccactt ccgaacttcc agctcacttc ctttcccact gcgagcgcat
    40321 taaagccacc aacggcaaag atcgggtgat catgcactgc cacgccacta acctgatcgc
    40381 tctcacctac gtgctggaaa acgacaccgc ggtcttcact cgccaactgt gggaaggcag
    40441 caccgagtgt ctggtggtat tcccggatgg cgttggcatt ctgccgtgga tggtacccgg
    40501 cacggacgaa atcggccagg caactgcaca agagatgcaa aaacattcgc tggtgttgtg
    40561 gcccttccac ggtgtgttcg gttccggacc gacactggat gaaaccttcg gtttaatcga
    40621 caccgcagaa aaatcagcac aaatattagt gaaggtttat tcgatgggcg gcatgaaaca
    40681 gaccatcagc cgtgaagagt tgatagcgct cggccagcgt ttcggcgtta caccactcgc
    40741 cagtgcgctg gcgctgtaag gagcaaacat gatccgcaaa gcctttgtca tgcaggtaaa
    40801 ccccgacgcc cacgaagagt atcagcgtcg gcataatccc atctggccgg aactggaagc
    40861 agtgctgaaa tctcacggtg cgcataacta cgccatctat ctcgacaaag cgcataatct
    40921 gctatttgcc acggtagaga ttgaatctga agaacggtgg aatgcggttg ccagtacaga
    40981 tgtttgccaa cgttggtgga aatatatgac cgacgttatg cccgctaatg cggataacag
    41041 cccagtcagt agcgaactgc aagaagtgtt ttacctgcct taatgagttg cggtgccgga
    41101 aaatatccag caccgccctt tcatcagaag aagtatttaa agcgaacgcg tgcgccgtaa
    41161 cggtcatcac tatcgtcgcc gtgtatctta tcgccgtcgc tatccagaat ggagtaatac
    41221 gtgccgaggt aaatattaaa gttctccata tccatcacat tagcgaactg ataagacgca
    41281 tgaatggtgt gaatgttgta tgttccttca tcatcaatcc agcaatcgtt atcacaaacc
    41341 acgccactaa attcatcaat cttattatgt gcatagatat aacccagctc gaaacgtttc
    41401 cacagcgcgt taatccctgc cgtgaaatct ttttcattat tagcatctaa ataggcggta
    41461 ttaagattaa ccacgatgcc attttccgga tcggttttca ggccattcca ggtcatactc
    41521 atgccataac cggtacgatc ggactgatcg acaaaattac ccttgctatc ggtataacca
    41581 taagcattat ttaccacatt cgcttccatc gctgcggaaa cggtgaattc ttccgtcggc
    41641 gaccaggcaa ttaccggacg cagataagca acatttttct gctgttccat atcgcgtcca
    41701 tgataattac cgtcgttata taaagatgtt ccgtcttcca gtaacgtgtt taattcaaaa
    41761 taccagttat cgagttgttt gctgacgagg aaattaccgc cagcgttaga acgtccgcgg
    41821 ccctctttca tcatatagat ataaccgctg ccatcgtcat aaagatcgtt cgcagtatta
    41881 ccggaatgtt caacaaaggt atcctgattc agcgggaaca tatcgtaggc ttcaaaacgg
    41941 cctactttga ccttccagtc gttttctttg ccaaagaaga aaaccgcatc atcgatattt
    42001 actgaaccgt gcatatcccc cagcggttgt gcggagaacc cagcgaaata gccattatcc
    42061 agttttcgca taccatcaaa acctaacaga atacgaccat tgagattcca ctgttcatta
    42121 gtgggatcgc tattcacatc agcgttggtc attgccagca ggccatgatt actttccgca
    42181 tccatattaa attctacgtc accgtagatt tttaaatcgc cataccctga aagcaggaga
    42241 ttaggcgggg tggcgttttt cttctctgac tccgtcgttg attgaccgtt taccggcgtg
    42301 ttgccctgtg cggttttaat ttcgcggatc tcggcggctt gttgtgtctg aagctgttta
    42361 acctgcccct ctgcttttgc tgcccgcatt tctgccgctt ccaggcgttc ttccagcaat
    42421 gccatgcgtt gttccagcgt taaatgctgt gtttttgcca taccggtact acatgccaat
    42481 aataaaccga tggctaaaga taatgtcgat tttttcatta tttacgttcc ctgaaataaa
    42541 ggagacccta cgctttccaa aaggaatacg tatatagaga aatataaggt gtgcactttc
    42601 gaatttttaa tccaacttca gcaggccgat ggatagtatc ccgccgcata atatgagtaa
    42661 atccgctgat ttcctggaac gtgaggaata taacaatagt gccgttttta actaaatatc
    42721 gtaaaaaata ttagatatga t
//
LOCUS       DAZFFE010000025        41155 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFFE010000025 DAZFFE010000000
VERSION     DAZFFE010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.025"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..454)
                     /locus_tag="ACHJ69_001613"
     CDS             complement(<1..454)
                     /locus_tag="ACHJ69_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO5014618.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLV"
     gene            complement(532..1524)
                     /gene="xylF"
                     /locus_tag="ACHJ69_001614"
     CDS             complement(532..1524)
                     /gene="xylF"
                     /locus_tag="ACHJ69_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5014619.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENTLKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            1890..3166
                     /gene="xylA"
                     /locus_tag="ACHJ69_001615"
                     /pseudo
     CDS             1890..3166
                     /gene="xylA"
                     /locus_tag="ACHJ69_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            3238..4692
                     /gene="xylB"
                     /locus_tag="ACHJ69_001616"
     CDS             3238..4692
                     /gene="xylB"
                     /locus_tag="ACHJ69_001616"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO5014620.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVAAQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMQALGVQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDLMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVIAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGVLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALITAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLL
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            4861..5202
                     /gene="yiaB"
                     /locus_tag="ACHJ69_001617"
     CDS             4861..5202
                     /gene="yiaB"
                     /locus_tag="ACHJ69_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO5014621.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFIHVCQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            5248..5685
                     /gene="yiaA"
                     /locus_tag="ACHJ69_001618"
     CDS             5248..5685
                     /gene="yiaA"
                     /locus_tag="ACHJ69_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO5014622.1"
                     /translation="MDNKISTYSPAFSIVSWVALIGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTPIYYMTCLTVFIISVALLLVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5726..6721)
                     /gene="wecH"
                     /locus_tag="ACHJ69_001619"
     CDS             complement(5726..6721)
                     /gene="wecH"
                     /locus_tag="ACHJ69_001619"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO5014623.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPITWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQSRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLYTRTICGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            6896..7195
                     /gene="ysaB"
                     /locus_tag="ACHJ69_001620"
     CDS             6896..7195
                     /gene="ysaB"
                     /locus_tag="ACHJ69_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO5014624.1"
                     /translation="MMMNAFFPAMALIVLVGCSTPPPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            7290..8201
                     /gene="glyQ"
                     /locus_tag="ACHJ69_001621"
     CDS             7290..8201
                     /gene="glyQ"
                     /locus_tag="ACHJ69_001621"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO5014625.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            8211..10280
                     /gene="glyS"
                     /locus_tag="ACHJ69_001622"
     CDS             8211..10280
                     /gene="glyS"
                     /locus_tag="ACHJ69_001622"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO5014626.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            10603..10755
                     /gene="hokA"
                     /locus_tag="ACHJ69_001623"
     CDS             10603..10755
                     /gene="hokA"
                     /locus_tag="ACHJ69_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO5014627.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACKLKE"
     gene            complement(10732..10803)
                     /locus_tag="ACHJ69_001624"
     CDS             complement(10732..10803)
                     /locus_tag="ACHJ69_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014628.1"
                     /translation="MRNTVRKAGIISRRRRLLFQFAG"
     gene            complement(10943..11155)
                     /gene="cspA"
                     /locus_tag="ACHJ69_001625"
     CDS             complement(10943..11155)
                     /gene="cspA"
                     /locus_tag="ACHJ69_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO5014629.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(11436..11726)
                     /gene="yiaG"
                     /locus_tag="ACHJ69_001626"
     CDS             complement(11436..11726)
                     /gene="yiaG"
                     /locus_tag="ACHJ69_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO5014630.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSDELKLMRLIQANPALSKQLME"
     gene            12160..12870
                     /gene="yiaF"
                     /locus_tag="ACHJ69_001627"
     CDS             12160..12870
                     /gene="yiaF"
                     /locus_tag="ACHJ69_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO5014631.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(12920..13894)
                     /gene="ghrB"
                     /locus_tag="ACHJ69_001628"
     CDS             complement(12920..13894)
                     /gene="ghrB"
                     /locus_tag="ACHJ69_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO5014632.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETYHLF
                     GAEQFGKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(13998..14657)
                     /gene="yiaD"
                     /locus_tag="ACHJ69_001629"
     CDS             complement(13998..14657)
                     /gene="yiaD"
                     /locus_tag="ACHJ69_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO5014633.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGSHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTSEGKAQNRRVEITLSP
                     L"
     gene            14810..17143
                     /gene="bisC"
                     /locus_tag="ACHJ69_001630"
     CDS             14810..17143
                     /gene="bisC"
                     /locus_tag="ACHJ69_001630"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO5014634.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETIDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMLVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRDEARNDF
                     DVFADLSERWEKGGYTRFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLAP
                     DEWQGSAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQTRGIQD
                     GDTVRLWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTAGGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(17112..17552)
                     /gene="yiaC"
                     /locus_tag="ACHJ69_001631"
     CDS             complement(17112..17552)
                     /gene="yiaC"
                     /locus_tag="ACHJ69_001631"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO5014635.1"
                     /translation="MIREVQRSELPAILQLWLESTTWGHPFIKSSYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYHAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(17549..18112)
                     /gene="tag"
                     /locus_tag="ACHJ69_001632"
     CDS             complement(17549..18112)
                     /gene="tag"
                     /locus_tag="ACHJ69_001632"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO5014636.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCHPGNKP"
     gene            18270..18968
                     /gene="yhjY"
                     /locus_tag="ACHJ69_001633"
     CDS             18270..18968
                     /gene="yhjY"
                     /locus_tag="ACHJ69_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO5014637.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHF
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            19197..20399
                     /gene="yhjX"
                     /locus_tag="ACHJ69_001634"
     CDS             19197..20399
                     /gene="yhjX"
                     /locus_tag="ACHJ69_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5014638.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLGAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTHLLE
                     TVGLEKTFVIWGAIVLVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDAISAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVIALVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFDGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
     gene            20723..21247
                     /gene="lpfA"
                     /locus_tag="ACHJ69_001635"
     CDS             20723..21247
                     /gene="lpfA"
                     /locus_tag="ACHJ69_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial major subunit LpfA"
                     /protein_id="HFO5014639.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGSVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTTTGDKSAATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     ISVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVATKNAVTI
                     GYGNAEVDFNLTYN"
     gene            21331..22017
                     /gene="lpfB"
                     /locus_tag="ACHJ69_001636"
     CDS             21331..22017
                     /gene="lpfB"
                     /locus_tag="ACHJ69_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFO5014640.1"
                     /translation="MKRISLAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQMSINGQKIERAGMVLAKGTLNINLPAGTGNANEVKYSIINDFGV
                     AGEMLTKSIN"
     gene            22040..24571
                     /gene="lpfC"
                     /locus_tag="ACHJ69_001637"
     CDS             22040..24571
                     /gene="lpfC"
                     /locus_tag="ACHJ69_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFO5014641.1"
                     /translation="MHWMHLPLDHYRAHFSFSLLALTIASALPAYGGKFNPKFLENVQ
                     GIDQHVDLSVYDSPVGQQIPGKYRVFVFVNEEKMASRTLDFSTASEAQRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKILPPEACVAFDEIIPQATSRFDFNTQTLHLTFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGSGSSPDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKESDGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSGKPRFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKDEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSIAYTWTRSPEWDED
                     DRLWSFSLSIPLGGAWGSYRMTTDQNGKTSQQASVSGTLLEDRNLNYNVQQGYTSNGV
                     GNSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAIVRSRFDTRVGYRVLMSLKRANGNAVPFGATAALSDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGHEQAQRCRVPFRLPEKKDNSGIVMVNAVCDK"
     gene            24582..25634
                     /gene="lpfD"
                     /locus_tag="ACHJ69_001638"
     CDS             24582..25634
                     /gene="lpfD"
                     /locus_tag="ACHJ69_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HFO5014642.1"
                     /translation="MKIKNLLAGMVLLGTSTFTGNIWAADWGPCRTASGDPFIFVTSF
                     TKNIQNPTDNVTGQTYPDFYQWALGDKYSGVCECPSPNPTEARPTLYKTESTLAAGHN
                     STYFKITNNLEVSTRVYIANVGNVQVPFINKSNSQPGRECDQPTFGWTTGSKGQLSLY
                     IAKPFVGEQNIPQTIIVSVFGTKKENVYSSVPISQVLLSGKVTVTQGCELAAGTSLDI
                     DFGEYQAHDFKGRTGQPPQNVQKIQKELTFNCTNISDGVHIYLSLEGTPNAAYPSAIS
                     LGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEYVKRKVTTTITAYPVSTTGKLP
                     AAGDYSGVATMHVELD"
     gene            25920..26099
                     /locus_tag="ACHJ69_001639"
     CDS             25920..26099
                     /locus_tag="ACHJ69_001639"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014643.1"
                     /translation="MRTIQIQQFPKNTTPTPIEFAINGDGAHQLNYIASYVPLRADAT
                     IGRADATVDFTVQYE"
     gene            26352..28043
                     /gene="eptB"
                     /locus_tag="ACHJ69_001640"
     CDS             26352..28043
                     /gene="eptB"
                     /locus_tag="ACHJ69_001640"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO5014644.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAREAAAN"
     gene            28135..28211
                     /locus_tag="ACHJ69_001641"
     tRNA            28135..28211
                     /locus_tag="ACHJ69_001641"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28169..28171,aa:Pro,seq:cgg)
     gene            28945..30552
                     /gene="dppA"
                     /locus_tag="ACHJ69_001642"
     CDS             28945..30552
                     /gene="dppA"
                     /locus_tag="ACHJ69_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO5014645.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPKIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(30614..30841)
                     /locus_tag="ACHJ69_001643"
     CDS             complement(30614..30841)
                     /locus_tag="ACHJ69_001643"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014646.1"
                     /translation="MTRSLELRVCIAHKSIPALVEQGMKYCCRMALHAEAQRHRVCRL
                     LTKSALFMPDAAQTPYPTYKIVQTQYIAEFS"
     gene            30859..31878
                     /gene="dppB"
                     /locus_tag="ACHJ69_001644"
     CDS             30859..31878
                     /gene="dppB"
                     /locus_tag="ACHJ69_001644"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO5014647.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            31888..32790
                     /gene="dppC"
                     /locus_tag="ACHJ69_001645"
     CDS             31888..32790
                     /gene="dppC"
                     /locus_tag="ACHJ69_001645"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO5014648.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            32801..33784
                     /gene="dppD"
                     /locus_tag="ACHJ69_001646"
     CDS             32801..33784
                     /gene="dppD"
                     /locus_tag="ACHJ69_001646"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO5014649.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNLVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDKCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            33781..34785
                     /gene="dppF"
                     /locus_tag="ACHJ69_001647"
     CDS             33781..34785
                     /gene="dppF"
                     /locus_tag="ACHJ69_001647"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO5014650.1"
                     /translation="MSTHEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSSLNPPPGCAFNARCRRRFGPCIQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(34815..36086)
                     /gene="yhjV"
                     /locus_tag="ACHJ69_001648"
     CDS             complement(34815..36086)
                     /gene="yhjV"
                     /locus_tag="ACHJ69_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO5014651.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLFYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASDFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYVTLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTRKINSLVLTLAICTFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     SQLEKLRGFKAWMILLYGILLCLSPLLKLIE"
     gene            36562..36669
                     /locus_tag="ACHJ69_001649"
     CDS             36562..36669
                     /locus_tag="ACHJ69_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5014652.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            37045..37152
                     /locus_tag="ACHJ69_001650"
     CDS             37045..37152
                     /locus_tag="ACHJ69_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5014653.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(37238..38917)
                     /gene="bcsG"
                     /locus_tag="ACHJ69_001651"
     CDS             complement(37238..38917)
                     /gene="bcsG"
                     /locus_tag="ACHJ69_001651"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO5014654.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLFVAWLFLSQWTRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDIPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSIFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQSELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQISGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQNKPYVRLNGGDWVPYPQ"
     gene            complement(38914..39105)
                     /gene="bcsF"
                     /locus_tag="ACHJ69_001652"
     CDS             complement(38914..39105)
                     /gene="bcsF"
                     /locus_tag="ACHJ69_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO5014655.1"
                     /translation="MMTISDIIEIIVFCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(39102..40673)
                     /gene="bcsE"
                     /locus_tag="ACHJ69_001653"
     CDS             complement(39102..40673)
                     /gene="bcsE"
                     /locus_tag="ACHJ69_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO5014656.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPGKITLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINSGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGCWTLVQHQEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLAQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            40946..41134
                     /gene="bcsR"
                     /locus_tag="ACHJ69_001654"
     CDS             40946..41134
                     /gene="bcsR"
                     /locus_tag="ACHJ69_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO5014657.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT        10318 a         9817 c        10591 g        10429 t
ORIGIN      
        1 caaccagttg ttgttgccca agtcctaaat cgccaacgcg ggtatcaggt gaaatggata
       61 aactgacctg tgcgagcagc ttctgacagc gtagcgtcat caggtcataa tccataatgc
      121 cattatgggt tatttcgtta cccaggaaga tattttccag cacggtcagt tctttcacca
      181 gcgccaattc ctggtgaata atggcgatac ctttgcgttc ggtatcgcgg atgtgactcg
      241 cctgaatctc ttctcccgca aaaataattt cgccttcgta ggaaccatgg ggataaatac
      301 cacacagcac tttcatcagc gttgatttac ctgacccatt ttccccacag agtgagacga
      361 tttcgccagc attcaaccgc aagctgacgt tatcaatcgc cttcacactg ccgaaggttt
      421 tggtaatgtt cttcatttca agtagataag gcataacgac tccacctaag ccaatcaatt
      481 cacgcggcat ggagagaaat cacgcccccg ctccgcgccg gggcgtaacg cttacagctc
      541 gctctctttg tggaatccgt ctttaactac cgtatctttg atgttgtttt tattcacatc
      601 gatcggtgtc agcaggcggg agggaacatc tttcaggcca ttattcagtg tggtatccgc
      661 ttttggctcc tgaccattgc ccaactcaac ggcaatttct gcggcagtat ttgccaacaa
      721 tgtaataggt ttatacaccg tcatagtttg cgtaccggca gcaatacgtt taatacctgc
      781 aagatccgca tcctggccgg aaattgctac tttccctgat aaaccttgcg cgcttaatgc
      841 ctgaattgcc cctcctgcgg tggcatcgtt tgaggcaact acagcatcaa ttttgttatt
      901 attggcggtt agcgcgtttt ccataatttt caatgtgttt tccggtaacc agccatcaac
      961 ccattggtca ccaacgactt taatttttcc ggaatcaaca taaggtttta atactttcat
     1021 ttgcccggcg cggaacagct tggcgttgtt atctaccggc gagccgccca tcaggaagta
     1081 attaccttgc ggaacaatat cgaccagggc ttttgcctgc agttcaccga ctttttcgtt
     1141 atcgaaagaa atataaaaat cgatatccgc atcgttaatc atacggtcgt aagctaatac
     1201 tttaatacct tcttgtttgg cttcttttac aacgttactt aatacctgac cgttataagg
     1261 aataatgaca agaacatcga caccccggtt aatcatgttt tcaatctgcg acatttgtgt
     1321 ttcttcattg ccatttgcag actgtacaaa tactttcgcg ccgagagatt ctgctttttt
     1381 cacaaaaata tctcgatctt tttgccagcg ttcaagacgg agatcatcaa tcgccatacc
     1441 tattttgact tctttggcgt gtgccgcaac gttggtaagc agaagtgagg tgcaaagggt
     1501 gagtagaatg ttctttattt tcatggtgta gggccttctg tagttagagg acagctttaa
     1561 taagtaacaa tcaccgcgat aaacgtaacc aatttttagc aactaaacag gggaaaacaa
     1621 ttacagattt ttatctttcg attacgattt ttggtttatt tcttgattta tgaccgagat
     1681 cttacttttg ttgcgcaatt gtacttattg catttttctc ttcgaggaat tacccagttt
     1741 catcatccca ttttattttg cgagcgagcg cacacttgtg aattatctca atagcagtgt
     1801 gaaataacat aattgagcaa ctgaaaggga gtgcccaata ttacgacatc atccatcacc
     1861 cgcggcatta cctgattatg gagttcaata tgcaagccta ttttgaccag ctcgatcgcg
     1921 ttcgttatga aggctcaaaa tcctcaaacc cgttagcatt ccgtcactac aatcccgacg
     1981 aactggtgtt gggcaagcgt atggaagagc acttgcgttt tgccgcctgc tactggcaca
     2041 ccttctgctg gaacggggcg gatatgtttg gtgtgggggc gtttaatcgt ccgtggcagc
     2101 agcctggtga ggcactggcg ttggcgaagc gtaaagcaga tgtcgcattt gagtttttcc
     2161 acaagttaca tgtgccattt tattgcttcc acgatgtgga tgtttcccct gagggcgcgt
     2221 cgttaaaaga gtacatcaat aattttgcgc aaatggttga tgtcctggca ggcaagcaag
     2281 aagagagcgg cgtgaagctg ctgtggggaa ccgctaactg ctttacaaac cctcgttatg
     2341 gcgcgggtgc ggcgacgaac ccagatcctg aagtcttcag ttgggcggtg aaaactatgt
     2401 cctgtggggc ggtcgtgaag gttacgaaac gctgttaaat accgacttgc gtcaggagcg
     2461 tgaacagttg ggccgcttta tgcagatggt ggttgagcat aaacataaaa tcggtttcca
     2521 gggcacgttg cttatcgaac cgaaaccgca agaaccgaca aaacatcaat atgattacga
     2581 tgctgcgacg gtctatggct tcctgaaaca gtttggtctg gaaaaagaga ttaaactgaa
     2641 cattgaagct aaccacgcga cgttggcagg tcactctttc catcatgaaa tagccaccgc
     2701 cattgcgctt ggcctgttcg gttctgtcga cgccaaccgt ggcgatgcgc aactgggctg
     2761 ggacaccgac cagttcccga acagtgtgga agagaatgcg ctggtgatgt atgaaattct
     2821 caaagcaggc ggtttcacca ccggtggtct gaacttcgat gccaaagtac gtcgtcaaag
     2881 tactgataaa tatgatctgt tttacggtca tatcggcgcg atggatacga tggcgttggc
     2941 gctgaaaatt gcagcgcgca tgattgaaga tggcgagctg gataaacgca tcgcgcagcg
     3001 ttattccggc tggaatagtg aattaggcca gcaaatcctg aaaggccaaa tgtcactggc
     3061 agatttagcc aaatatgctc aggaacataa tttgtctccg gtgcatcaga gtggtcgcca
     3121 ggagcaactg gaaaatctgg taaatcatta tctgttcgac aaataacggc taactgtgca
     3181 gtccgttggc ccggttatca gtagcgatac cgggcatttt tttaaggaac gatcgatatg
     3241 tatatcggga tagatcttgg cacctcgggc gtaaaagtta ttttgctcaa cgagcagggt
     3301 gaggtggttg ctgcgcaaac ggaaaagctg accgtttcgc gcccgcatcc actctggtcg
     3361 gaacaagacc cggaacagtg gtggcaggca actgatcgcg caatgcaagc tctgggcgtt
     3421 cagcattctc tgcaagacgt taaagcatta ggtattgccg gacagatgca cggtgcaacc
     3481 ttactggatg ctcagcaacg ggtgttacgc cctgccattt tgtggaacga cgggcgctgt
     3541 gcgcaagagt gcactttgct ggaagcgcga gttccgcaat cgcgggtgat taccggcaac
     3601 ctgatgatgc ccggatttac tgcgcctaaa ttgctatggg ttcagcggca tgagccggag
     3661 atattccgtc aaatcgataa agtattatta ccgaaagatt acttgcgtct gcgtatgacg
     3721 ggggagtttg ccagcgatat gtctgacgca gctggcacta tgtggctgga tgtcgcaaag
     3781 cgtgactgga gcgatctcat gctgcaggct tgccacttat ctcgtgacca gatgcccgca
     3841 ttatacgaag gcagcgaaat tactggtgct ttgttacctg aagttgcgaa agcgtggggt
     3901 atggcggcgg tgccagttat cgcaggcggt ggcgacaatg cagctggtgc ggttggtgtg
     3961 ggaatggttg atgctaatca ggcaatgtta tcgctgggga cgtcgggggt ctattttgct
     4021 gtcagcgaag gggtcttaag caagccagaa agcgccgtac atagcttttg ccatgcgtta
     4081 ccgcaacgtt ggcatttaat gtctgtaatg ctgagtgcgg cttcgtgtct ggattgggcc
     4141 gcgaaattaa ccggcctgag caatgtccct gctttaatca ctgcagctca acaggctgat
     4201 gaaagtgccg agccagtttg gtttctgcct tatctttccg gcgagcgtac accacacaat
     4261 aatccccagg cgaaaggggt tttctttggt ttgactcatc aacatggccc caatgaactg
     4321 gcgcgagcag tgctggaagg cgtgggttat gcgctggcag atggcatgga tgtcgtgcat
     4381 gcctgcggca ttaaaccgca aagtgttacg ttgattggtg gcggggcgcg tagtgagtac
     4441 tggcgtcaaa tgctggcgga catcagcggt cagcaactgg attaccgcac gggtggcgat
     4501 gtggggccag cactgggcgc agcaaggctg gcgcagattg cggcgaatcc agagaaatcg
     4561 ctcattgagt tgttgccgca actactgtta gaacagtcgc atctaccaga tgcgcagcgt
     4621 tatgccgctt atcagccacg acgagaaacg ttccgtcgcc tctatcagca acttctgcca
     4681 ttaatggcgt aaacgttatc ccctgcctga ccgggtgggg gataattcac atctatatat
     4741 ctcagtaatt aattaatatt tagtatgaat ttattctgaa aatcatttgt taatggcatt
     4801 tttcagtttt gtctttcgtt ggttactcgt aatgtatcgc tggtagatat ggagatcgtt
     4861 atgaaaacct caaagactgt ggcaaaacta ttatttgttg tcggggcgct ggtttatctg
     4921 gttgggctat ggatctcatg cccattgtta agtggaaaag gttattttct tggcgtgtta
     4981 atgacggcaa cttttggcaa ctatgcatat cttcgcgcag aaaaacttgg gcaactggat
     5041 gattttttta tccacgtctg tcagttggta gcgttaatca ctatcggtct cttgtttatc
     5101 ggtgttttaa acgcacctat caatgcttat gaaatggtga tctaccccat cgcctttttt
     5161 gtctgcttgt ttggtcaaat gcgtttgttt cgctcggcat gagcaacata aagttcttac
     5221 acattcagga atgaaaggaa tactgtgatg gacaacaaaa tatcaaccta ttctccggca
     5281 tttagtattg tttcatgggt agcccttatt ggcggtattg ttacctatct gttaggacta
     5341 tggaatgcag agatgcagtt aaatgaaaaa gggtattatt ttgccgtact ggtattaggt
     5401 ttgttttccg cggcgtctta tcaaaagacc gttcgggaca agtatgaagg catacctacc
     5461 acccccattt attatatgac ttgcctgacg gtctttatta tctctgttgc gttgttgctg
     5521 gtaggtctat ggaatgcaac gttattactc agcgaaaaag gcttttatgg gctggctttc
     5581 ttcttaagct tgtttggtgc agtagcggtg cagaagaata ttcgtgatgc cggaataaac
     5641 ccaccaaaag aaacacaggt tacccaggaa gaatacagcg aataattcac ataagcccgg
     5701 ttagtccaat gtaaccgggc ttttactaac tcactaatct gtttctgtcg attcgttgta
     5761 ccagcataga aagtaacaaa ctcgctgcca acgtcgcgca aaagatccaa ataatatcga
     5821 gtattggcca gtttttaagc tcaatccccc gggtgcgcag tgcatggata atcaatgcat
     5881 ggaacccgta tatacctaac gaatggcggg agattaagcc aagtccgcaa atggtacgcg
     5941 tatacagcgt gtttttaacc agagtcaata acgcgattgc gcagataaaa accatcggcc
     6001 cacagtaaag ataccaggta tcggcaaaat ttccgcgcca ctgtaattca tataatgtcc
     6061 cgcgagagat aataaaaacc cccgtcgcaa acagtgcggc gctcacccac gacagtgctt
     6121 tatgctgtgt gtccatcatc cctatagcgc ggcccaacat gccataaagg atgtagtaaa
     6181 aagtatcgcc attgatatat aagttaattg gcagccattc aaaaccgtcg attttctgcg
     6241 gcacagtatt tgggttagcg atgataccaa tcaccaccat tagcaccagc aacatttttc
     6301 cgccgacgtt cttcacctga atcagcggtg aaaccagata aatcactgca atcgcgaaga
     6361 aaaaccataa atggtaaaac actggctttt gcagcaggtt tttcagcgct aactccacat
     6421 tgatggaggt aaacagcgca atgtagagca gcgcgattgc gctataaaaa aacaggcata
     6481 agccgatacg caagaaatgg cgcgactggg cgctgcgttc gccaaaaaag agatagccgg
     6541 aaatcatgaa aaatagtggc acgctgacac gagaggcaga attcagaaca ttggcgatat
     6601 cccaggtgat ggggctaaca ctatgagcat tggtcacata ccaggtagtg gtgtgaatca
     6661 tcaccaccat taaacacgct atccctcgca ggttatcaat ccagtaaatt ttgggctgca
     6721 tctgtgtctc tgtatctggt taaaaaaagt ctgaccgata aatcattcag ttggcgcact
     6781 ggaataatct gagttttatc actacagctt atagaggctt aaggaaattc gtaagatatc
     6841 agccactata ccgatataaa taataagact cacctgcaaa ccagacggta atttaatgat
     6901 gatgaacgct ttctttccgg caatggcgct tattgtgcta gtgggttgtt ctacaccgcc
     6961 acccgtgcag aaagcacaac gggtaaaggt tgatcctctg cgttcgttga atatggaagc
     7021 gttatgcaag gatcaggcgg caaaacgtta taacaccggc gagcaaaaaa tcgacgtcac
     7081 cgccttcgaa cagttccagg gaagctatga aatgcgcggc tataccttcc ggaaagagca
     7141 gtttgtctgt tcttttgacg cggatggcca ttttttgcat ctttccatgc gttaagccct
     7201 gctttttccc gtttcgtact gtatatcttc catccagcgg gtatactgat cccttccttt
     7261 aaatccacac gtatccagca cgaaataata tgcaaaagtt tgataccagg accttccagg
     7321 gcttgatcct gaccttacag gattactggg ctcgccaggg ctgcaccatt gttcaaccat
     7381 tggacatgga agtcggcgcg ggaacctctc acccaatgac ctgtctgcgc gcgctggggc
     7441 cagaaccgat ggcggctgct tatgttcagc cttctcgtcg cccgaccgat ggtcgctacg
     7501 gcgaaaaccc caaccgttta cagcactact atcagttcca ggtggtcatt aagccatcgc
     7561 cggacaatat tcaggagctg tacctcggtt ctctgaaaga gctgggcatg gacccgacca
     7621 tccacgacat ccgtttcgtg gaagataact gggaaaaccc gacgctgggt gcctgggggc
     7681 tgggctggga agtgtggcta aacggcatgg aagtgacgca gttcacttac ttccagcagg
     7741 ttggtggtct ggaatgtaaa ccggttaccg gtgagatcac ctacggtctg gaacgtctgg
     7801 ccatgtacat tcagggcgta gacagcgttt acgacctggt ctggagcgac ggcccgctgg
     7861 gtaaaaccac ctacggcgac gtgttccatc agaacgaagt ggagcagtcc acttacaact
     7921 tcgaatacgc ggatgtggac ttcttgttca cctgcttcga gcagtacgag aaagaagcgc
     7981 agcagttgct ggcgctggaa aatccgctgc cgctgccagc ctacgagcgt attctgaaag
     8041 ccgcccacag cttcaacctg ctggatgcgc gtaaagccat ctccgtcacc gagcgtcagc
     8101 gctacattct gcgcattcgc accctgacca aagcagtggc agaagcatac tacgcttccc
     8161 gtgaagccct cggcttcccg atgtgcaaca aagataagta agaggcggct atgtctgaga
     8221 aaacttttct ggtggaaatc ggcactgaag agctgccacc aaaagcacta cgcagcctgg
     8281 ctgagtcctt tgctgcgaac tttactgcgg agctggataa cgctggcctc gcacacggca
     8341 ccgttcaatg gtttgctgct ccgcgtcgtc tggcgctgaa agtagctaac ctggcggagg
     8401 cgcaaccgga tcgtgaaata gaaaaacgcg gcccggcaat tgcccaggcg ttcgacgctg
     8461 aaggcaaacc gagcaaagcg gcagaaggtt gggcgcgtgg ttgcggtatt accgttgacc
     8521 aggctgagcg tctgactacc gataaaggcg aatggctgct gtatcgcgcc catgtgaagg
     8581 gtgaaagcac cgaagcactg ctgccgaata tggttgcgac ttcgctggcg aaactgccga
     8641 tcccgaaact gatgcgttgg ggcgcaagcg acgtgcactt cgtgcgtccg gttcacaccg
     8701 tgaccctgct gctgggcgac aaagttattc cggctaccat tctgggtatt cagtccgatc
     8761 gcgtgattcg cggccaccgc tttatgggcg agccggaatt cactatcgac aatgccgatc
     8821 agtatccgga aattctgcgt gagcgcggga aagtcatcgc cgattacgaa gaacgtaagg
     8881 cgaagattaa agccgatgct gaagaagcag cacgtaagat tggcggtaac gctgatttaa
     8941 gcgaaagcct gctggaagaa gtggcttcgc tggtggagtg gccggtcgtt ctgaccgcga
     9001 aattcgaaga gaaattcctc gcggtgccgt ctgaagcact ggtttacacc atgaaaggtg
     9061 accagaagta cttcccggtg tatgcgaacg acggcaaact gctgccgaac tttatcttcg
     9121 ttgccaacat cgaatcgaaa gatccgcagc agattatctc cggtaacgag aaagtcgttc
     9181 gtccacgtct ggcggatgcc gagttcttct tcaataccga ccgtaaaaaa cgtcttgaag
     9241 ataacctgcc gcgcctgcaa accgtgttgt tccagcaaca gttggggacg ctgcgcgaca
     9301 aaactgaccg cattcaggcg ctggctggct ggattgctga acagattggc gctgacgtta
     9361 accacgctac ccgtgcgggc ctgctgtcta agtgtgacct gatgaccaac atggtcttcg
     9421 agttcaccga cacccagggc gttatgggga tgcactacgc gcgtcacgat ggcgaagcgg
     9481 aagacgtagc tgtcgcgctg aacgaacagt atcagccgcg ctttgccggt gatgacctgc
     9541 cgtctaaccc ggtagcctgt gcgctggcga ttgctgacaa gatggatacc ctggcgggta
     9601 tcttcggtat cggccagcat ccgaaaggcg acaaagaccc gtttgcgctg cgtcgtgccg
     9661 cacttggcgt gctgcgtatt atcgttgaga agaacctcaa ccttgatctg caaacgctga
     9721 ccgaagaagc agtgcgtctg tatggcgata agctgactaa cgccaacgtg gttgatgatg
     9781 ttatcgactt tatgctcggt cgcttccgcg cctggtatca ggacgaaggt tacaccgttg
     9841 acaccatcca ggcggtactg gcgcgtcgtc cgactcgtcc ggctgatttc gatgcccgta
     9901 tgaaagcggt atcgcacttc cgtaccctgg aagcagctgc agcactggcg gcggcgaaca
     9961 agcgtgtatc taacatcctg gcgaaatctg atgaagtgct gagcgatcgc gtgaatgcct
    10021 ctactctgaa agagccggaa gaaattaaac tggcgatgca ggttgtggtg ctacgtgaca
    10081 agctggagcc gtactttgcg gaaggtcgtt accaggatgc gctggtcgaa ctggctgagc
    10141 tgcgtgaacc ggtcgatgct ttcttcgata aagtgatggt catggttgat gataaagaat
    10201 tgcgtatcaa ccgtctgacc atgctggaaa aactgcgcga actgttcctg cgcgttgcgg
    10261 atatttcgct gttgcagtaa taacgccgtt attaaaaagc ctgccatctg gcaggctttt
    10321 tttatcgcta aataatacag caacctttaa taatcttctg ctgaataaag attgtctcat
    10381 atattaattt catgagattt ttttaggatt atatcaagga gaagaaacga acttattaag
    10441 ctagaatagc tgcgggtgct tgagactgtt tgtctcaggt attcaccgaa aggcagacag
    10501 agaaaagccc cacctgacta taaatcaaag tgaggcccct ctatgcatag acaccataag
    10561 gttagcctct tactcgttgg aaatcaacac agggggctgg gaatgccgca gaaatataga
    10621 ttactttctt taatagtgat ttgtttcacg cttttatttt tcacctggat gataagagat
    10681 tcactgtgtg aattgcatat taaacaggag agttatgagc tggcggcgtt tttagcctgc
    10741 aaattgaaag agtaagcgtc ttcggcggga aattattccc gcctttctta cggtgttgcg
    10801 cattctcatt gcacccaaat ttattcttca caaaaataac actagattct attacgcgat
    10861 cgattattta tttcctgaaa aacaaataaa aaaatccccg ccaaatggca gggatcttag
    10921 attctgtgct tttaagcaga gattacaggc tggttacgtt accagctgcc gggcctttag
    10981 cgccgctttc gatggtgaag gacactttct gaccttcgtc cagagatttg taaccatcgt
    11041 tctggatagc agagaagtgt acgaacacgt ctttagagcc atcgtcagga gtgatgaagc
    11101 cgaagccttt gtcagcgttg aaccatttta cgataccagt cattttaccg gacatagtgt
    11161 attaccttta ataattaagt gtgcctttcg gcgatatggc gtgctttaca gattttgaag
    11221 cgttaaagga atgtgcacta cgaggggtat caacgataac tcttgaaggg acttgcctta
    11281 ctacactgct ttaatggtct gtacgtcaaa ccgttgatgt gcattaagcc acgcattggc
    11341 gggtgatgca acaattattt ttcatattta tgattaatcg gttggaaaac ggtcctgtta
    11401 tcaggaccgt aaacagcaat aaagtggata aaagtctatt ccatcaactg cttacttaat
    11461 gccgggttgg cttgaatcaa acgcatcaat tttagttcgt cacttgaagg cttcacgcgt
    11521 ctggattccc attcctttac catggcgact gatacgccca aaacccgggc aaaatcatcg
    11581 atttttaatc ctgtcccttt tcgtaactgc tcaatttcgg tacaggatgt tgttctgtgc
    11641 gtcagggtaa ttttctgcgt ttcatcttta aaaacaatct gttccaggct gctcaacagc
    11701 tcatgcattg gatctttata ttccattgaa aactcctcaa atcacactgc gggatcgtga
    11761 attgcatcga agctcattaa gaatagtcgg gaaaaacaga cacagcgggg tgtagtcagg
    11821 gcatactctg actatgattc gattcagcca gtcatttagc gtgacttata gccacctaac
    11881 gatctgataa atgccgatat atctcttacc actcccggtt tgtaaagatt tcgaaaatct
    11941 aacgttctgc gaaggcgtaa atctctggct acaaaaaagg caacggcaat atcatttttt
    12001 aacaatgaaa caacaaatta accttgtttc attttctcca tgattttacc aggaagatta
    12061 cgtagaaagg gtatcctgca agcctgtcct ggactatcct tgtcacgtca gacactcgtg
    12121 tgtcgttgtg cgcttttttt gggtgaaagg agtaagaaaa tggcgacagg aaagtcctgc
    12181 tctcgctggt ttgcgcctct tgcggcgtta ttaatggtag ttagcctgag tgggtgtttt
    12241 gataaagaag gcgatcagcg taaagcgttt atcgacttcc tgcagaatac agtgatgcgt
    12301 agcggtgaac gtctaccaac cctgactgcc gatcagaaaa aacagtttgg tccttttgtc
    12361 tctgattacg cgattctgta tggttattct cagcaggtga atcaggcgat ggattccggt
    12421 ctgcgcccgg ttgtagacag cgttaacgca attcgcgtac cgcaggatta cgttacgcaa
    12481 agcggtccgc tgcgtgaaat gaacggttcg ctgggtgtgc tggcgcaaca gctgcaaaat
    12541 gcgaagttgc aggccgatgc tgcgcactct gcgttaaaac agagtgatga cctgaaaccg
    12601 gtctttgatc aggcattcac caaagtggta acgacgccag ctgatgcatt gcaaccgttg
    12661 attccggcgg cacaaacttt cacgcaacaa ctagtgatgg ttggagacta cattgctcag
    12721 caaggtactc aggtgagctt tgtcgcaaat ggcatccagt tcccgacttc acagcaggca
    12781 agtgaatata acaaactgat tgcgccatta ccggcacaac atcaggcgtt taatcaggcc
    12841 tggactacag cagttactgc aacccaataa agagtaaaag cccgagcgat tatcgggctt
    12901 tactcctcgc agtcgcggct tagtccgcga cgtgcggatt cacacagttc ttctcaacct
    12961 ttccttgtaa cgcatcaatc aaattatcca cggcacaggc ggccatgcca taacgcgtct
    13021 catgagtggc agatccaata tgcggtactg cgacgacgtt ggccattgag agcaacggcg
    13081 aatctacgga cagtggctct tgttcgaaga catccagccc ggcggcgtga atttctcctt
    13141 tctgcaatgc agcaatcagt gcattttcat caaccaccgg gccacgtccg gcattaatga
    13201 aaatggcgga ggatttcatt ttgccgaatt gttctgcgcc aaatagatga tacgtctcat
    13261 cagttaacgg caggatcagg caaacgaaat ctgactcttg taacagtgta tccaaatcgc
    13321 agtagcgggc gttgaagcgt tcttctgctt ctttatggtg gcggcgcgcg ttatagagga
    13381 tgggcatgtt gaaaccaaag tgcgcacgtt gtgccagcgc catgccgatc cgtcccatcc
    13441 cgacaatgcc cagtgtttta tggtgaacgt cagtgccgta ccagtccggg cctatgctcg
    13501 cggtccattc gcctgctttt acccgttctg ctacctccac aacccgacga gcggtagaca
    13561 acaccagcgc catcagcgta tcggcgacgg tttctgttaa tacggttggt gtgtgcatca
    13621 gcagaatttt tcgggcggta agcgcatcga catcaaaatt gtcatagccg acggagatcg
    13681 ttgatgtggc acgcagtttc ggcatttttt ccagcaatgc ggcatcaaca ttctcgtttg
    13741 aacccagcaa gccttcagct tcggcaaaaa ttgctgcatt ttgttcgacg gtttgtgggc
    13801 tgaggtttgc cacctggtga acggtgaaat gttcttgcag gcgttgcagt aaatcatcag
    13861 gtaaggcttt gtagaggata acggacggct tcatgcttct ctccattcac tgataaggcg
    13921 atcaccttag cctgaaatat gatgaaggaa aactgaagaa tgccaggtga tttctgcatc
    13981 acctgacatg aaagggatta cagtgggctt aaggtaattt ctacacggcg gttttgcgcc
    14041 ttaccttctg aggtgctgtt gctggcgatt gggttagccg ggccaaggcc ttgagtacgg
    14101 atgcggctgg cgtccacgcc ctgggtgatc aacgcgctgg caacggaatc cgcacgctgc
    14161 tgggagagac gcatgttcag gtcgtgactc cccgtgctgt cggtataacc aatcacgtta
    14221 accgccgttt tcggatactc tttcagtacc attgccacgc cggtcagggt gttagcgccc
    14281 gccggtttca gggttgcgct gctgctgtcg aaggtcacat tgttcggcat attgaggata
    14341 atgttatccc cgctgcgggt tacgctaaca ccagtgccgc gcattttgtc acgcagcttc
    14401 gcttcctgca catccatgta ataaccaacg ccgccgccca gagctgcgcc tgctgctgcg
    14461 ccaatgagcg cgcctttacc gcgatctttc ttcgaagaag agagcgcacc aatacccgcg
    14521 ccaacgagag agcccagacc tgcgccgata gcagatttac ctgcttcgcg ttcgccggtg
    14581 taagggttag ttgtacagcc agataccgcc agagcgccac tcactacggc ggcaataaga
    14641 taaacacgtt tcttcattgt taatccttaa taaccttttt attctttgcc acgggttccg
    14701 tggcgggaga ttatgccgcg tgagcatgaa gattattcct gggaatactc ggaaatttgt
    14761 aagtaatatt taactgctca atacatctaa cctttcagga gtcttcggtt tggccaactc
    14821 atcctcacga tattccgttc tgactgccgc ccactggggg cctatgctgg ttgaaaccga
    14881 cggcgaaacc gtgtttagct cgcgtggcgc gttagccaca ggaatggaaa actccttgca
    14941 gagcgcggtt cgcgaccagg ttcacagcaa tacgcgggtg cgatttccaa tggtgcgcaa
    15001 aggctttctt gcgtcaccgg aaaatccgca aggcattcgt gggcaggatg aatttgttcg
    15061 cgtgagttgg gatgaggcgc tggagcttat tcaccatcaa cataaacgca ttcgtgaggc
    15121 ttatggtccg gcatcgattt ttgctggttc ctacggctgg cgttcaaacg gcgtgctgca
    15181 taaggcctcg acattattac aacgctatat ggcgctggca ggcggttata ccgggcatct
    15241 gggggattat tcgaccggcg cggcacaggc gatcatgccg tatgtcgtgg gtggcagcga
    15301 agtttatcaa cagcaaacca gttggccact ggtgctggaa catagcgatg tcgtggtgct
    15361 gtggagtgct aacccactca atacgctgaa aattgcgtgg aatgcatccg atgagcaggg
    15421 gctttcttac ttttccgcac tgcgtgacag cgggaaaaag ctgatctgca ttgatccaat
    15481 gagatcggaa accatcgatt tctttggcga taaaatggag tgggtggcac cacacatggg
    15541 caccgatgtt gcgttgatgc tggggatcgc ccatacgctg gtggaaaatg gttggcacga
    15601 cgaagcgttt ctggcgcgtt gcaccacagg ttatgccgtc ttcgcctctt atttgctggg
    15661 cgagagtgac ggtatagcga aaaacgccga atgggcggca gagatttgtg gtgttggcgc
    15721 agcgaaaatc cgcgagctgg cggctatttt ccaccaaaat acaaccatgc tgatggcagg
    15781 ctggggaatg cagcgccaac agtttggtga gcaaaaacac tggatgctcg tcacgctggc
    15841 ggcaatgttg gggcaaatcg gcacacccgg cggcggtttt ggtctttctt accattttgc
    15901 caatggtggt aaccccacgc gccgcgctgc ggtgctctct tccatgcagg gcagcttgcc
    15961 gggtggcacc gatgcggtag ataaaatccc tgtcgcccgc attgttgaag cactggaaaa
    16021 ccctggtggc gcatatcaac ataatggtat ggaccgacat ttcccggata ttcgttttat
    16081 ctggtgggcg ggtggtgcca actttactca tcatcaggat accaatcgcc tgatccgtgc
    16141 ctggcaaaaa ccggagctgg tggtgatctc cgaatgcttc tggacggctg cggcgaaaca
    16201 cgcggatatc gttctgcctg cgacaacctc ttttgagcgt aatgatctca ccatgaccgg
    16261 tgattacagt aatcagcatc tggtgccgat gaagcaagtg gtgccgccac gcgatgaagc
    16321 gcgtaatgat tttgatgttt ttgccgactt gagtgaacgt tgggagaagg gcggttatac
    16381 acgttttacg gaaggaaaaa gtgagctgca atggctggaa acgttttata acgttgcccg
    16441 gcagcgcggg gcaagccagc aggttgaatt gccaccattt gctgagttct ggcaagccaa
    16501 ccagttaatt gagatgccgg aaaacccgga cagtgagcgg tttattcgct tcgccgattt
    16561 tcgccgcgat ccgcaggcgc atccattaaa aaccgccagc ggtaagattg aaatcttctc
    16621 gcagcgtatt gccgattacg cttatccgga ttgcccgggg catccaatgt ggctggcgcc
    16681 ggacgaatgg cagggcagtg ccgaaccgga acagttgcag gtactttctg ctcatccggc
    16741 gcatcgtctg cacagccagc tgaattacag ttctctgcgc gaattgtacg cggtggcaaa
    16801 tcgtgagcct gtcaccattc atcctgacga tgcccagacg cgcggcatac aagatggcga
    16861 tactgttcgg ctgtggaact cacgcgggca aattcttgcc ggagcggtca ttagcgaggg
    16921 aattaaacct ggcgtgattt gtattcacga aggcgcatgg ccggatttgg atttaaccgc
    16981 tggcggcatt tgtaaaaacg gcgcagtgaa cgtgctgacc aaagatctcc ccagctcgcg
    17041 gctggggaat ggctgtgcgg gtaatacggc gctggcatgg ctggaaaaat acaacggtcc
    17101 ggaactgaca cttacagcgt ttgaaccacc ggccagctca taatccaggt gggtagttgg
    17161 gtttcatcct gccatgcgca atcgacaatg tgaaaaccct gcgcatggta gaaatctatc
    17221 gccggttgat ttttttgata aacctccagc atcagatggg gatggcgctg ctgcacatac
    17281 tgcatcagcg ccttaccaat accgcgcctg acggccttcg gtgcgacaaa cattgctgcc
    17341 agaaatcggc cttccataat actgacaaaa ccgagaagct tgccgtcttc ttcccagacc
    17401 cagttttgcg cgttggcaag atacgcatcg cgcaccagcg gaatgcagtc acgccagtaa
    17461 ctcgatttta taaagggatg cccccaggtt gtactttcca gccacagttg gaggatcgcg
    17521 gggagttctg aacgttgcac ttcccgaatc atggtttatt tcccggatgg caacagcagc
    17581 caaccacatg atcattcacc agcccacatg cctgcataaa ggagtaacag attgtggtac
    17641 cgacaaactt aaaaccacgt ttttttagtg ccttagatag ggcgtcggag gcgggtgtag
    17701 atgtggggat ttcgctcaac gttgtggctt gtgtcacctg tggttgatga ttgacaaacg
    17761 accagacaaa gtcggcaaac ggttcgccgt tctgttccat ttgcaggtac gcccgcgcat
    17821 taccaataat tgcctgaatt ttcccacgat ggcggataat cccggcgtcc tgtaccagtc
    17881 tttcgacatc ctcttcctgc attgcggcaa ctttcaccgg atcgaactga tgaaagcatg
    17941 cgcgatagtt ttcgcgtttt ttgaggacgg tgatccacga taacccagcc tgctgccctt
    18001 ccaggcagat catttcgaac agttttttac tgtcagtttc aggcacgccc cattcattat
    18061 catggtaggc aatataaagc gggtcctgac tcacccagcc gcaacgttcc atactttccc
    18121 tcgctatgta cccgatcgta aaaatttcag ccgcttttct tgacgcatcg gtgaaaaagt
    18181 caatatttaa tacagggcta ctatgcccga tatccttcat acaatgacaa taatattgcc
    18241 gaattcggct cttccctgga gtcgtgttaa tgatcataaa aaaaagcggt ggtcgctggc
    18301 agctaagcct gcttgcgagc gtggtaatca gtgccttttt tctcaacaca gcttacgcct
    18361 ggcaacaaga atatatcgtt gatacgcaac ccggacattt cacagagcgt tacacctggg
    18421 atagcgatca tcaacctgat tacaacgata ttttgtcgca acgtattcaa agtagccaaa
    18481 gggcgctggg actggaagtc aatctggcgg aagaaacccc tgtggatgtg accagcagta
    18541 tgagtatggg ctggaatttt cctttatatg agcaggttac aaccggcccg gtcgcggcat
    18601 tacattacga tggcacaact acctcgatgt ataacgagtt tggcgacagt actaccacgc
    18661 tggccgatcc gttatggcat gccagcgtga gtaccttagg ctggcgtgtt gactcccggc
    18721 ttggcgatct tcgaccctgg gcgcaaatca gctataacca gcaatttggc gagaatatct
    18781 ggaaggcgca atcaggcctg agccggatga cggcgacaaa ccagaacggc aactggctgg
    18841 atgtcaccgt aggcgctgat atgttgctca atcaaaatat tgctgcctat gctgcgctat
    18901 ctcaggcaga aaataccact aacaatagcg actatctgta tacgatgggg gttagtgcca
    18961 gattttaacg ttatagatac aattgaaacc cataaataac aactaatgtg gtgatagtgg
    19021 ggactaactt actaaataat aaatttggcg aataattgtc aagtcattca ttcctgaact
    19081 aatgcatttc attccgttct gatggcattt catgccgttt ttccccaggc ataaagcgca
    19141 cttcgttatg gttgtcagca gagatttttc ctttttatta ctgcaggaat actgccatga
    19201 caccttcaaa ttatcagcgt actcgctggc tgacactcat cggtactatc attacccagt
    19261 ttgctctggg gtcggtttat acctggagtc tgtttaacgg tgcgctttct gccaagctgg
    19321 gggctcctgt aagccaggtt gctttctctt tcggcttgtt aagtctgggg ctggcaattt
    19381 cgtcttctgt tgcgggcaaa ttgcaggaac gttttggtgt taaacgcgtc actatggctt
    19441 ccggcatttt gctgggatta ggtttcttcc tgacagcaca ttccaacaac ctgatgatgc
    19501 tgtggttaag cgccggtgtg ttggttggtc tggcggatgg tgcgggttac ctgttgacgc
    19561 tctctaactg cgtgaagtgg tttccggagc gtaagggact tatctctgct tttgctatcg
    19621 gttcttatgg tctgggcagc ctgggtttta aatttatcga cacgcacctg ctcgaaacgg
    19681 tgggtctgga aaaaaccttt gttatttggg gcgcgattgt actggtgatg attgtctttg
    19741 gcgcaacgtt aatgaaagat gcgccgaagc aggaagtgaa aaccagcaat ggtgtggtgg
    19801 agaaggacta caccctggca gagtcgatgc gtaaaccgca gtactggatg ttagcggtta
    19861 tgttcctgac tgcgtgcatg agtggtctgt atgtgattgg tgtagcgaaa gatatcgctc
    19921 aaagcctggc gcatcttgat gcaatttccg cagccaatgc tgtgacggtt atttccatcg
    19981 ccaacctttc tggtcgcctg gtgctgggca ttctgtctga caaaatcgcc cgtatccgtg
    20041 ttatcaccat tggtcaggtg attgcgctgg tgggtatggc ggccctgctg tttgcaccat
    20101 tgaatgcagt gacgttcttt gcagcgattg cctgtgtggc atttaacttt ggtggcacga
    20161 ttacggtgtt cccgtcactg gtcagtgagt tcttcggcct caataacctg gcgaaaaact
    20221 acggcgtcat ttacctcggt ttcggtatcg gcagcatttg tggttcgatt atcgcttcac
    20281 tgtttgacgg attctatgtg actttctacg tgattttcgc cctgctgatt ctgtcactgg
    20341 cgctttctac gaccattcgc cagccagagc agaaaatgtt gcgtgaagca catatttaga
    20401 aatatgttgc tgctgaaata aatacgatga gggagacttc gaagtctccc ttttttatgg
    20461 ttgcatgtta aaagaacaat ggcatccaaa aaatgaatat ttttcgttta tacattcttt
    20521 atgtaaatat aaatattttc ttaaaaaaac ggtaattaaa ttgcgtttta tgtgtaattg
    20581 attgaagttg aagcatattt caacttattg caaaaattaa tattcagtaa aaataatttt
    20641 attttttaac gccatttgta taatctgcga cccgttgtat gaacaacatc aagcatattt
    20701 atctatataa atggagttat aaatgaaaaa ggttctgttt gccttatctg cgcttgcgct
    20761 gacctctact tccgtattcg cagctgatgc aggcgacggt tctgttaaat tcaccggaga
    20821 gattgttgac gccgcttgtg ttgtgtctcc agacactcaa aaacaagaag ttgttctggg
    20881 ccaggtgaac aagtctgtgt ttaccactac tggtgataaa tccgcagcaa ctccgttcaa
    20941 aatcaaactg gaaaactgcg atatctccac tttcaaaaat gtggagatca gctttaacgg
    21001 tgttggcgac gcagacaaca gcaaactgat ttctgtaagc actgaaccag gtgccgcaac
    21061 tggcgtgggt attggtattt atgataatac caatacgctg gttgatctga ataccggtaa
    21121 atctgctact gtcctgaaag aaggccagac tgttctgtat tttaccgcta actatgtcgc
    21181 taccaaaaat gcagtaacca tcggttacgg taatgccgaa gtcgacttca acctgactta
    21241 caactaatcg atccagacgg aacgattatc tctcaatggc aacagattat ctgttgccat
    21301 tattcacacc aatgactcag ggaccgaatc atgaaacgaa ttagccttgc cgttctggcg
    21361 cttttaatga ccgcacaaca gtgttttgct ggcggcgtag ccctaagcac gacgcgcgtt
    21421 atttatgatg gtggcaagaa agaagcgtcg ctgaccgttg aaaaccataa taaaaatgaa
    21481 gagtttttga tccagtcatg gattgatgat gtgaatggaa ataaaaaaac gccgtttatt
    21541 attacaccgc cattattcaa acttgatcct gataaaaata acgtcttacg tattgttaat
    21601 attgaagata ccttacccaa agatcgtgaa tccgtttact ggattaatgt aaaagcgatt
    21661 cctgcgaaaa gtgctgaaaa tgaaaataaa aacgtcttac agatagccgt tcgcacccgt
    21721 ttgaagttgt tctaccgtcc gaaaggcttg ccgggaagca accttgaagc aatgaaacag
    21781 ttacgatttc agcgtcaaaa taaccaaatt gtggttaata acgactcagc ttataacctg
    21841 acctttaatc agatgtccat taatggacaa aagattgaac gggcgggaat ggtcctcgct
    21901 aaaggaacgt taaacattaa ccttccagcc ggtacaggca atgcgaacga agtgaaatac
    21961 agcattatta atgatttcgg tgtcgccggt gaaatgctga cgaagtcgat taactgatct
    22021 ttttacaagg cgctttacta tgcattggat gcatttacct cttgatcact acagagcgca
    22081 tttttctttc tcactgttgg cattaactat tgccagcgcg ttacctgcgt acggcggaaa
    22141 attcaaccct aaatttcttg aaaatgttca gggtattgat caacacgtcg atttgtcagt
    22201 ttacgactct cctgtcggcc agcaaatacc cggtaaatat cgggtatttg tgtttgtaaa
    22261 cgaagaaaaa atggcttccc ggacgctgga ttttagtact gccagcgaag cacaacgcaa
    22321 agccagcggc gagtcgctta tgccttgcct tagccgtgtt caactggaag aaatgggcgt
    22381 gcgcgtcgac agctttccgg cactgaagat attgccaccg gaagcctgcg tagcgtttga
    22441 tgaaatcatt ccccaggcca ccagtcgttt tgattttaat actcagacgt tgcatcttac
    22501 ctttccacag gcagcaatga tgatgaccgc tcgtggcacg gttgatcctt ctcgctggga
    22561 cgaaggaata cctgcgctgt tactggacta cagctttagt ggcagcaatg gtcgcaacga
    22621 aggttccggt tcatctccag attcgaccag tgacagttat tacctgaacc tgcgcagcgg
    22681 tttgaacgtg gggccgtggc gtttacgtaa taacagtatc tggaaccgta ccgatggcaa
    22741 aaatcaatgg gataacgttg gtacatcgtt aaaccgggcg attattccac tcaagtcaca
    22801 gataacattg ggtgataccg ccacgcctgg cgagattttt gatagcgtac aaatgcgcgg
    22861 ggcattattg gcatccgacg atgaaatgtt gccggatagc cagcgtggct ttgctccggt
    22921 ggtgcgtggc atcgccaaaa gtaacgctga agtgagtatc gaacagaacg gatatgttat
    22981 ttatcgtacc tttgttcagc cgggcgcatt tgagattaac gatctctacg caacctccgg
    23041 cagcggtgac ttgacggtga tcattaaaga atctgacgga agtgaacaac gttttattca
    23101 accattttcg gcagtggcga ttttccagcg tgaagggtat ctgaaataca gccttgctgc
    23161 cggtgagtat cgtgccggta attacgacag cggtaaaccg cgattcgggc aatttactgc
    23221 catgtacggc ttgccgtggg gaatgaccgc ctatggtggt gcgttgctat cggcagacta
    23281 caacgcttta gctctggggc tggggaaaaa cttcggcacc attggtgcgg tatcggtgga
    23341 tgtcacccag gcgaaaagtc agttacgcaa caatgaaaaa gatgaagggc agtcttatcg
    23401 tttcctctat tcgaaaagtt ttgagggcgg taccgacctg cgtctgttgg gctacaagta
    23461 ctcaaccagc ggttattaca ccttccagga agcgaccgat gtccgtagtg acgccgacag
    23521 tgactatcgg cgttatcata agcgcagcca gatacaaggt aacatcactc agcaactggg
    23581 agactacggt tctgtttact tcaatatgac gcagcaggat tactggaacg ttgatggtaa
    23641 ggagaactcc ttaagcgctg gttatcacgg tcatattggt cgtgtgaatt acagtatcgc
    23701 ctatacctgg acgagaagcc ctgaatggga tgaagacgat cgactgtggt catttagcct
    23761 ctcaataccg ctgggtggcg catggggcag ttatcgtatg accaccgacc agaacggcaa
    23821 gacgtcacaa caggcctctg tcagcggcac attactggaa gatcgtaact tgaactataa
    23881 cgttcagcag ggttatacca gtaatggggt tggcaacagt ggtagtgtca acatgggtta
    23941 tatgggaggt tctggcaaca ttgacgtggg ttataactat tccaaagata accaacaagt
    24001 taactatggt gtgcgtggtg gagtcattgt tcacagtgaa ggtatcactc tctcgcaacc
    24061 cttgggtgaa agtctggcaa ttgtttctgc gccgggtgcg cgtggtggac atgtagtaaa
    24121 tagcagcggc gttgaagttg actggatggg caacgcagtg gtgccgtatc tcacgccata
    24181 tcgtgaaacc attgtggaat tgcgcagcga cacgttaggg caaaacgttg aattacagga
    24241 agcgtttcag aaagtcgttc caacgcgtgg ggcgatagtc cgttcccgct ttgatacgcg
    24301 tgtcggttat cgtgtgctga tgagtttgaa acgggcgaat ggcaacgcag taccgtttgg
    24361 tgctaccgca gcgttaagtg atgagagcaa acctgccagc agcattgtgg gtgaagaagg
    24421 acagctttat atcagcggga tgccagaaga gggcgaattg caggtgagct gggggcacga
    24481 gcaggcgcaa cgttgccgcg tgccattccg cttgccagag aaaaaggata attcgggtat
    24541 cgtgatggtt aatgcggtat gcgataaata aggatatgca gatgaaaatt aaaaacttgt
    24601 tggcaggtat ggtgctttta gggacgagca cctttaccgg gaatatatgg gctgcggatt
    24661 gggggccgtg caggacggca agtggtgacc cctttatttt tgttacatca ttcaccaaaa
    24721 atattcaaaa tccgacggat aatgttaccg ggcagaccta ccctgatttt taccaatggg
    24781 cactgggtga taaatattct ggtgtttgtg aatgtccatc accgaacccg acagaagcac
    24841 gaccaacgct atataaaact gagtcaacat tggcggcggg gcataactca acctatttta
    24901 aaattaccaa taatcttgag gtttcaaccc gggtatatat tgcaaacgtg gggaatgtac
    24961 aggttccatt tatcaataaa tcaaatagcc agccaggaag agagtgcgat caaccaactt
    25021 ttggctggac tacagggagt aaaggacagc tctcacttta tatcgctaaa ccttttgttg
    25081 gggaacaaaa cattccgcaa actattatag tttctgtgtt tggtacaaaa aaggagaatg
    25141 tttatagttc agttccaata tcacaggtgc ttctttcagg aaaggtcacc gtaactcaag
    25201 gctgtgaact tgccgcaggg acatcattgg atatcgactt tggtgagtat caggcccatg
    25261 atttcaaagg tcgaaccggg cagccgccac aaaatgtcca gaaaatacaa aaagaattaa
    25321 cgtttaactg tactaatatt tctgatggtg tccatatcta tctgagtctt gaaggtacgc
    25381 caaacgcggc ttaccccagc gcaatcagtt tgggcaacgc ggatgttggt gcggtaattg
    25441 aggatggtaa aggtaatatt cttaagccga atgatagtaa tagcttatta gaaatgaatc
    25501 ccggctcact gtacgaatat gtcaaacgta aagtaacaac caccattact gcttatccgg
    25561 ttagtacaac cgggaaatta cccgcagccg gtgattatag cggtgtggca acaatgcatg
    25621 ttgaattgga ttagcactga tgaaacgaac aatacttgca ttatgcctgc tacctttttc
    25681 cttttcgtca ctgtcggcaa ccacagactt aggggcgaaa ggaacgttga aattctctct
    25741 taaaatcagc cagggagcct gtgaactggt aaaagacagt gttgaagttg acatgggatt
    25801 agcggttctc aaaaagccag taagaatcgg aacagaactt aatcccacta ctttttccat
    25861 cggtctgaaa aactgtagcg aagttgtcag ggcttacgtc accatggatg gaacacccaa
    25921 tgcggacgat ccaaatacaa cagttcccga aaaatacgac accgacgccg attgagttcg
    25981 cgattaacgg tgatggcgcg catcaactga actatatcgc cagctacgtg ccgctgcgtg
    26041 ctgatgcaac cattggccgc gcagatgcga cggtcgattt tacggttcag tacgaataat
    26101 ttcgcttcac cggggctagc tcactggccc tttttactgc tttttgtaac gctaaagtct
    26161 cttttcaaac ttgcattttt gtaaatttgt gcttcgtgca cactctttcc ccacactttt
    26221 tccctttgct gtggtctact tattcgcgcg tgtagatttt acttatctga ctacctccgc
    26281 acttttcccc tgcctggcct gaaaagccac taagcagggt gttatcacct gtttgtccag
    26341 ggcttgtttg catgagatac atcaaatcga ttacacagca gaagctgagc ttcttgcttg
    26401 caatctatat tggccttttt atgaatggcg cggtttttta ccgccgcttc ggcagctatg
    26461 cgcacgattt taccgtctgg aaaggtattt ctgctgttgt tgaactggcc gccaccgtac
    26521 tggtgacctt ctttttactg cgtcttcttt cgctgtttgg ccgccgcagc tggcgtattc
    26581 tggcatcgct ggtggtgctc ttttccgcag gtgccagcta ttacatgacc ttccttaatg
    26641 tagtcattgg ttatggcatc atcgcatccg tcatgaccac cgatatcgac ctgtcaaaag
    26701 aagttgttgg tctgaacttt attctctggt taatcgccgt aagcgcgttg cctcttatcc
    26761 ttatctggaa taaccgctgt cgttacacct tgctccgaca actgcgaacc ccagggcaac
    26821 gtattcgcag cctggcggtc gtcgtactgg caggtattat ggtttgggca ccgattcgtt
    26881 tgctggatat ccagcagaag aaagtggaga gggcgacagg tgttgatttg ccgagttatg
    26941 gcggcgtcgt cgcgaactct tatctgccat caaactggct ttcggcatta ggtctttatg
    27001 cctgggcgcg ggtcgatgaa tcttccgata acaattctct ccttaaccct gcgaagaagt
    27061 ttacgtacca ggctccacaa aacgttgatg atacatatgt cgttttcatt attggtgaaa
    27121 ccacgcgctg ggatcatatg ggcattttcg gctatgaacg caataccaca ccgaagctgg
    27181 ctcaggagaa aaatctggcg gcgttccgtg gttactcctg tgatactgca accaaactct
    27241 cactgcgttg catgtttgta cgtcagggtg gagcagaaga taatccgcag cgcacattaa
    27301 aagaacagaa cattttcgcg gtattgaagc agttaggatt cagttctgat ctctacgcta
    27361 tgcaaagcga aatgtggttc tacagcaata cgatggcgga caacattgct tatcgtgagc
    27421 agattggtgc ggagccacgt aaccgtggca agccggtaga tgatatgttg ctggtagacg
    27481 aaatgcagca atcgctgggg cgcaacccgg atggtaagca tctgatcatt ctgcatacca
    27541 aaggctcaca ctttaactac acccagcgtt acccgcgcag ctttgcgcag tggaagccgg
    27601 aatgtattgg tgttgatagc ggctgtacca aagcgcagat gatcaactca tacgacaact
    27661 cggtgaccta tgtggatcac tttatttcca gcgtgattga tcaggttcgc gataagaaag
    27721 cgattgtgtt ctatgcagct gaccacggtg agtcaattaa tgaacgcgag cacctgcacg
    27781 gcacgccgcg tgaactggca ccgccggaac agttccgtgt gccgatgatg gtctggatgt
    27841 cggataaata tctggaaaat ccggtcaatg cgcaggcatt tgcgcagctg aaaaaagaag
    27901 ccgacatgaa agtgccacgc cgtcatgtag agctgtacga caccatcatg ggttgtcttg
    27961 gctatacttc accggatggt ggaattaacg aaaacaacaa ctggtgccac atcccgcagg
    28021 caagagaggc ggcggctaac taagaggcct gctgactttc tcgccgatca aaaggcattt
    28081 tgctattaag ggattgacga gggcgtatct gcgcagtaag atgcgccccg cattcggtga
    28141 ttggcgcagc ctggtagcgc acttcgttcg ggacgaaggg gtcggaggtt cgaatcctct
    28201 atcaccgacc aaattcgaaa agcctgctca acgagcaggc ttttttgcat ctgtaactca
    28261 tgaggaagag aacctccggg ggcggggagg ttcgactcga cgtaggcctg ataagacgcg
    28321 ccagcgtcgc atcaggcaaa tgtaccggcc cattgtcgga tgcggcgcga ccgccttatc
    28381 cgatctacac accacacatt tccctgccaa atttgcgccc catatctcac tatccattaa
    28441 cggatttgtg acagaaacca ttgtcttttt ttatatttgc ttaaatcttt ttttgcgtta
    28501 cttatggctg gcgctggcat tttccgctgt gcgttttagc gttcatggta ttaatcgctc
    28561 agatatccac ctttacctca gatttttttc attaaatgca taaaacttaa tcaccaaatg
    28621 ttgctaaata ttaagctgat tgttctggtt gtgttggcgt agcacaaatt tctctgctgc
    28681 aaatagctgt tttaatcttt gtttgtggat tctcaccgtt ggtgtaaatc ccggttggct
    28741 gtattgacgt tttcacattc tgttgacaga ttgtaggtca cgaggggcat tttatggagg
    28801 atccgcactg ttacactgat gttaattagt acggcatccc cacctcataa cgttgacccg
    28861 accgggcaaa aaacaaaaaa ggtcaggcag cgacaaccca ctgcaaaggg ttaaaacaac
    28921 aaacatcaca attggagcag aataatgcgt atttccttga aaaagtcagg gatgctgaag
    28981 cttggtctca gcttggtggc tatgaccgtc gcagcaagtg ttcaggctaa aactctggtt
    29041 tattgctcag aaggatctcc ggaagggttt aacccgcagc tgtttacctc cggcaccacc
    29101 tatgacgcct cttccgtacc gctttataac cgtctggttg aatttaaaat cggcaccact
    29161 gaagtgatcc cgggcctcgc tgaaaagtgg gaagtcagcg aagacggtaa aacctatacc
    29221 ttccatctgc gtaaaggtgt gaagtggcac gacaataaag aattcaaacc gacgcgcgaa
    29281 ctgaatgccg atgacgtagt gttctcgttc gatcgtcaga aaaacgcgca aaacccgtac
    29341 cataaagtct ctggcggcag ctacgaatac ttcgaaggca tgggcttacc agagctgatc
    29401 agcgaagtga aaaaggtgga cgataacacc gttcagtttg tgctgactcg cccggaagcg
    29461 ccgttcctcg ctgacctggc aatggacttc gcctctattc tgtcaaaaga atatgctgac
    29521 gcgatgatga aagccggtac accggaaaaa ctggacctca acccaatcgg taccggcccg
    29581 ttccagttac agcagtacca gaaagattcc cgtattcgct ataaagcgtt tgatggctac
    29641 tggggcacca aaccgaagat cgatacgctg gtcttctcta ttactcctga cgcttccgtg
    29701 cgttacgcga aattgcagaa gaacgaatgc caggtgatgc cgtacccgaa cccggcagat
    29761 atcgcccgca tgaagcagga taaatccatc aacctgatgg aaatgccggg gctgaacgtc
    29821 ggttatctct cgtataacgt gcagaaaaaa ccactggatg acgtgaaagt tcgccaggct
    29881 ctgacctacg cggtgaacaa agacgccatc atcaaagcgg tttatcaggg cgcgggcgta
    29941 tcagcgaaaa acctgatccc gccaaccatg tggggctata acgacgacgt tcaggactac
    30001 acttacgatc ctgaaaaagc gaaagccttg ctgaaagaag cgggtctgga aaaaggtttc
    30061 tccatcgacc tgtgggcaat gccggtacaa cgtccgtata acccgaatgc tcgccgcatg
    30121 gcggagatga ttcaggcaga ctgggcgaaa gtgggcgtgc aggccaaaat cgtcacctac
    30181 gaatggggtg aatacctcaa gcgtgcgaaa gatggcgagc atcagacggt aatgatgggc
    30241 tggactggcg ataacgggga tccggataac ttcttcgcca ccctgttcag ctgcgccgcc
    30301 tctgaacaag gctccaacta ctcaaaatgg tgctacaaac cgtttgaaga tctgattcaa
    30361 ccggcgcgtg ctaccgacga ccacaataaa cgtgttgaac tgtacaaaca agcacaggtc
    30421 gtgatgcacg atcaggctcc ggcactgatc atcgctcact ccaccgtgtt tgaaccggta
    30481 cgcaaagaag ttaaaggcta tgtggttgat ccattaggca aacatcactt cgaaaatgtc
    30541 tctatcgaat aattaaagcc atgcaagact gatggcaaag gcaaaactgc ctgatgcgct
    30601 ccgcttatca ggcctacgaa aactctgcaa tgtattgagt ttgcacgatt ttgtaggtcg
    30661 gataaggcgt ttgcgccgca tccggcataa acaaagcgct ctttgtcaac aatctgcata
    30721 cccggtggcg ctgtgcctct gcgtgcagcg ccatccggca gcaatacttc attccctgtt
    30781 ccacaagggc gggaattgat ttgtgagcaa tacagacacg cagttccagg ctgcgggtca
    30841 ttatagagaa tccgggttat gttgcagttt attctccgac gtttgggact cgtcatcccc
    30901 acgtttatcg gtattaccct tctcacattt gcctttgtcc acatgatccc gggcgatccg
    30961 gtgatgatca tggcgggcga acgtgggatc tccccagagc gtcacgcgca attgctggct
    31021 gaactcggct tagataaacc gatgtggcag cagtatctcc attacatttg gggcgtaatg
    31081 cacggtgatt taggcatttc gatgaaaagc cgaattccgg tatgggaaga gttcgtgccg
    31141 cgctttcagg ccacgctgga acttggcgtc tgcgcgatga tttttgcgac ggcagtcggt
    31201 attccggttg gtgtgctggc tgcggttaaa cgtggttcca ttttcgatca cacagcggtt
    31261 ggcctggcgc tgaccggcta ctcgatgcca attttctggt ggggcatgat gctgatcatg
    31321 ctggtttcgg tgcactggaa cctgacgcct gtctccggtc gcgtgagcga catggtgttc
    31381 ctcgatgatt ccaatccgtt aaccggtttt atgctgatcg acaccgccat ctggggtgaa
    31441 gacggtaact ttatcgatgc cgtcgcccat atgatcctgc ccgccattgt gctgggtact
    31501 attccgctgg cggtcattgt gcgtatgacg cgctcctcga tgctggaagt gctgggcgaa
    31561 gattatatcc gcaccgcgcg tgccaaaggt cttacccgga tgcgggtgat tatcgtccat
    31621 gcgctgcgta acgcgatgct gccggtggtg accgttatcg gcctgcaggt gggaacattg
    31681 ctggcggggg cgattctgac tgaaaccatc ttctcgtggc ccggtctggg gcgctggttg
    31741 attgacgcac tgcaacgccg cgattatccg gtagtgcagg gcggcgtatt gctggtggcg
    31801 acgatgatta tcctcgtcaa cctgctggtc gatctgctgt acggcgtggt gaacccgcgt
    31861 attcgtcata agaagtaagg ggacatcatg tcacaggtta ctgaaaataa agtgattagc
    31921 gcaccggtgc cgatgacccc gttacaggag ttctggcact attttaaacg caacaaaggc
    31981 gcggttgtcg ggctggttta cgtcgtcatc gtgctgttta tcgcgatctt tgccaactgg
    32041 attgcaccct ataacccagc ggaacagttc cgcgatgcac tgctcgcccc gccagcctgg
    32101 caggaaggcg gcagcatggc gcacttgctg ggcactgatg acgtggggcg tgatgtgctg
    32161 tcgcggctga tgtacggcgc gcgcttgtct ctgctggttg gctgtctggt agtggtgtta
    32221 tcgctgatta tgggcgttat tctcggcctg atcgctggtt actttggcgg tctggtcgat
    32281 aacatcatca tgcgcgtggt cgatatcatg ctggcgctgc caagtctact gctggcgctg
    32341 gtgctggtgg cgattttcgg cccgtcgatt ggtaacgccg cgctggcgct aaccttcgtt
    32401 gcgctgccgc attatgtgcg cttaacccgc gccgccgtgc tggtggaagt taaccgcgat
    32461 tacgtcaccg cgtctcgcgt ggcgggtgcc ggggcaatgc gccagatgtt tattaacatc
    32521 ttcccgaact gccttgcgcc gctgattgtt caggcgtcgc tcggtttctc taacgccatt
    32581 ctcgatatgg ctgctcttgg tttcctcggc atgggcgcgc agccgccaac gccggagtgg
    32641 ggcaccatgc tctctgatgt gttgcagttc gcgcaaagcg cctggtgggt cgtgaccttc
    32701 ccgggtctgg cgatcctgct gacggtgctg gcatttaacc tgatgggtga cggtctgcgt
    32761 gacgcgctcg atcccaaact gaagcagtaa gaggttcgag atggcgttat taaatgtaga
    32821 taaattatcg gtgcatttcg gcgacgaaag cgcaccgttc cgcgccgtag accgcatcag
    32881 ctacagcgta aaacagggtg aagtggtcgg gattgtgggt gagtccggct ctggtaaatc
    32941 ggtcagttcg ctggcaatta tggggctgat tgattatccg ggccgcgtaa tggcggaaaa
    33001 gctggagttt aacggtcagg atttgcagcg tatctcagaa aaagagcgcc gcaacctggt
    33061 gggtgccgaa gtggcaatga tcttccagga cccgatgacc agccttaacc cgtgctacac
    33121 cgtgggtttc cagattatgg aagcgattaa ggtgcatcag ggcggcaaca aaagtacccg
    33181 ccgtcagcga gcgattgacc tgctgaatct ggtcggtatt cccgatccgg catcgcgtct
    33241 ggatgtttac ccgcatcagc tttccggcgg catgagccag cgcgtgatga tcgccatggc
    33301 gattgcctgt cgaccaaaac tgctgattgc cgatgaaccg accactgcgc tggacgtgac
    33361 catccaggcg caaatcatcg aactgctgct ggagttgcag cagaaagaga acatggcgct
    33421 ggtgttgatc actcatgacc tggcgctggt ggcggaagcg gcacataaaa tcatcgtgat
    33481 gtatgcaggc caggtagtag aaacgggcga tgcgcacgct atcttccatg cgccgcgtca
    33541 cccgtatact caggcattgc tgcgtgcgct gccggaattt gctcaggaca aagaacgtct
    33601 ggcgtcgttg ccaggtgtcg ttcccggcaa gtacgaccgc ccgaacggct gcctgcttaa
    33661 cccgcgctgc ccctacgcca ctgacaaatg ccgtgctgaa gaaccggcgc tgaatatgct
    33721 cgctgacggg cgtcagtcca aatgccatta cccacttgat gatgccggga ggccgacact
    33781 atgagtacgc atgaggccac ctcgcaacaa ccgctgttgc aggctatcga cctgaaaaaa
    33841 cattatccgg tgaagaaagg catgttcgcg ccggaacgtc tggtgaaggc gctggatggc
    33901 gtttcgttta accttgaacg tggcaaaacg ctggcagtag taggtgaatc tggctgcggt
    33961 aaatcgaccc tcggtcggtt gctgacgatg attgaaacgc ccaccggcgg cgaactgtat
    34021 taccaggggc aggatctgct caagcacgat ccgcaggcgc agaagctacg tcggcagaaa
    34081 atccagattg tcttccagaa cccttacggt tcgctgaatc cgcgtaaaaa agttgggcag
    34141 attctcgaag agccgctgct gatcaacacc agcttaagca aagaacagcg tcgggaaaaa
    34201 gccctgtcga tgatggcgaa agtcggcctg aaaaccgagc attacgaccg ctatccgcat
    34261 atgttctccg gcggtcagcg tcagcgtatc gccattgccc gaggtctgat gctcgacccg
    34321 gatgtggtga ttgccgatga gccggtttct gcgctggacg tctcagtgcg tgcgcaggtg
    34381 ctgaatctga tgatggattt gcagcaggaa ctggggctat cttatgtctt tatctcgcac
    34441 gatctgtcgg tggtggagca cattgctgat gaagtgatgg tgatgtacct gggccgctgc
    34501 gtggagaagg ggacgaagga ccaaatcttc aataacccgc gccacccgta cactcaggca
    34561 ttgctctccg cgacaccacg cctgaacccg gacgatcgtc gcgaacgcat caaacttacc
    34621 ggtgaactgc caagctcgct gaatccaccg ccaggctgcg ccttcaacgc ccgctgtcgt
    34681 cggcgcttcg gcccctgcat ccagttgcag ccgcagctaa aagactacgg cggtcaactg
    34741 gtagcctgtt ttgccgttga tcaggatgaa aatccgcagc gttaactcac aaaaccgggg
    34801 ccatgcgctc cggtttactc aatcaacttc aacagtggcg acaagcataa caaaatgccg
    34861 tacagcagaa tcatccaggc tttaaacccg cgaagttttt ccagttgtga gactttatag
    34921 atcaggaaaa acggaatgag gcacgacaca atgccatata acgggcttcc caactgaaag
    34981 aacaccagta ccgatacacg aaacgaaacc caaatcgtca acgtaatgac gataaaagtg
    35041 cagatcgcca gagtcagcac gagtgagtta attttcctgg tatcaataat tcggcttaac
    35101 agattgagaa taatgccttt aatggcctcg tggaaaccga gataaatgcc aaagaatgca
    35161 gtcagtacgg caaagatatt cagcaccgta gaggtgatat gaatgatatg cccagggatc
    35221 acctgcgcgg ccagtgccag tgccgagata ttttgttcaa aggcagaaac ggcttcttcg
    35281 tggctaattg agaaggtaaa cgaaaaggca aaaaacagga tcaccgcgat gagcgtgaca
    35341 taactaattc ggtgggtacg cagcgcgagc cgggttgcca gtactttatc cgcttcccgt
    35401 ttacgatagg caatattcat tgggttaagt acctgaataa ataccgcgga aaagaagcaa
    35461 aatggaatag taagcaggac atcgcggaaa aagtcggagg cttgcgggaa ggcggttata
    35521 ttggcgaaat tccagtgcgg gatcatcgca aaaccgaaca cgacaataat ccctactttg
    35581 accaccacca ttggtccgga aatcttaaac agtaatcgtt caccaccaga cgcaatcgcc
    35641 accagtacgg cgaaaatggc gactttataa aatagagatt gagaaagatc ggcatcggtt
    35701 aaaccgaagg ttttcaggta cgaggcgctg tcgaaaacca cggagagaga gtagataaaa
    35761 atcccgtgga taatcatcaa aaagtagata accccgagga aaattcccca gttcttcccc
    35821 aggtaatggc tgataatatc ggtgtagtca ttacaggaat cgctttcaga aagggttttt
    35881 aaataaatgt cctgtactac ccaggtggca ggataagcaa tgatcgctgc ggtaataaat
    35941 acccaaattc ccttcaagcc aatttgtact ggcatcagca cggttccggc accaatcgcc
    36001 atgcctatgc ataataaaac ccagccgaag tcgtagcgtg taaacggcaa tttctgatcg
    36061 tgtttcggta gtgtgttgtg ctgcataaag tcctgtccag aaagaagtcg gcctcgcaaa
    36121 aatgaataaa cccagtttct cttgcgcaac aggtggagaa gtcgaatacg cagtatgtaa
    36181 aaaatgaggc aacagattca ctggggaggt cgcgaggagg cgcaattcta caaaattgcg
    36241 catttcacta ctagtctgga gtcggcccgg atatgagctg caactattgc aaataaagaa
    36301 attaagctgg aaggcatcac gcaatgagga agattgccgg gtgctgacgg caaaaagcaa
    36361 tcgtaaaatc agcggcaagc cggaagggtt ccggtgaggc gcaatgttgc gggggcttta
    36421 tccctggtgg cattggttgc tggagagaga aaacccccgc acgttgcagg tatgcacctg
    36481 acaacatcac gggggccatt cttgcatcta gaccattcaa gaatagccgc gaaacgttgt
    36541 cattacaaca caggcggcta tatgacgctc gcagagctgg gcatggcctt ctggcatgat
    36601 ttagcggctc cggtcattgc tggcattctt gccagtatga tcgtgaactg gctgaacaag
    36661 cggaagtaac gtgtcatgcg ggcgtcaggc tgccgtaatg gcaatttgcg cccggaccag
    36721 gccgcagagg tgaaactctg cggccttttt cgtaagaaaa atgcaacggt tgcaagcgga
    36781 taatattctg cggaatgcgc agcacagagt gaagaagtga cggattccgg agcagaaaaa
    36841 cagtcgtaga atcagcggca agccggaaag gttccggtga ggcgcaatgt tgcgggggct
    36901 taatccctgg tggcattggt tgctggagag agaaaacccc cgcacgttgc aggtatgcac
    36961 ctgacaacat cacgggggct aatcttgaac ctagaccact caagaatagc cgcgaaacgt
    37021 tgtcattaca acacaggcgg ctatatgacg ctcgcagaac tgggcatggc cttctggcat
    37081 gatttagcgg ctccggtcat tgctggcatt cttgccagta tgatcgtgaa ctggctgaat
    37141 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
    37201 caggccgcag ggggaaactc tgcggccttt ttcgttctta ctgcgggtaa ggcacccagt
    37261 cgccgccgtt caggcgaacg tacggtttat tctggtattg aataactact gcatttgagt
    37321 tctcggagac cggtgctgtt tgtggtaacc cactggtgag ttttttccag tcaacattgt
    37381 cttcggtgaa aatcttgcca tcaagaacgc gaaccaccag atcggagata gccaggaagc
    37441 tgctcggttg gtcgatgaca atcggagccc cctgatgtgg tgccttcatg ccgaagaatt
    37501 tcaccccaac ggggacgtct gtgatagacg ggctagggat atcacgtaga ccagatatct
    37561 gcattctgtc gcccttcagc gcgccgccgt gttccggcac cactaccacc atcactttac
    37621 gacccgattt ctccagttca gtaaagaagg cgtccagttc atcaaagaat ttttgcgccc
    37681 gcgctttgta atccgctgtt ttgctgacgc ccggataatg gttgccgtca tgcagtggaa
    37741 gcgtgttgta gaacgtggca ctacggctgt ttttatcttt ttcggtaacg tccagccaac
    37801 ggttaagcac ggcggtatcg tcataaaccg gcgaaccatc aaagcccagc aaaataaccg
    37861 gcagatttgt ttgatccatc aattcgctct gcatgccgcc attttcgcga acttccttca
    37921 aaaaaccacc gaactggccg ttatgtccca tcatcaggtg ctgggtaaag cccagtttcg
    37981 acaggttatc aaacagatag cagtcgttat ttgccggttg atacagatta gtgtgcgaag
    38041 tctgcccgca gctggcgcgc agtaaacgga tcgccgccgg gccactgtag gaggtggcgg
    38101 agttaaagtt cttgaactca atatcgaaat gcgaccacag tggatgcgac atcaatccgg
    38161 cggcttcaat atccgaccag gaaagcgaac agatattaat taccagtagt tcaaatggct
    38221 gagcatcagc gggcagcgaa gacgggaaga tcgatttacg tttcgcctcc gcgttataga
    38281 aattattcag ccaggcgtta aggtttgctg ttgttggcgg tgcagtttgt gcgggtatat
    38341 cacccactac cggtgcgcca cccgtcgccg caacggttgc cgctgcgtta ccacccgtcg
    38401 ttgttacagt ggtcgtcggt tgaccggctg gccacaagga gaaacttggt cccgccaggg
    38461 taagtacgtt cagccatagc agaatggcaa ccacaaaaac ggtaatgcga gtccattgtg
    38521 acaggaataa ccaggccacg aataaaacaa aaatggcccc aatcatctgc cagttaataa
    38581 agcgtgtgac aagatcgatt aaataatcgg tactgaaccc cgccacctgc gaaccctggc
    38641 tcattatgct ttccgggcca ggcaaccagg tgtcatgcca gaacaaagca aagccgatcg
    38701 gcagggcaat ccagtggcgc aagcgatgca ggctgtagcg cggaatgggc atcagcagaa
    38761 acgcggcaaa caccagattg aggagcggat ggaagttaag atatcccgcc cacaacaggc
    38821 cgaacttaac cagaaaatag aagttccagc cggaaaggcc gcgccagtat tgccagaggg
    38881 aagaaggcat ggcggtattt tgcgtaaatt gagtcatttt ttggttgccc tggctttttc
    38941 cgtgcggcgt aacgtcccgg ccggtttaac ataacgaggt ttagcaaaga acaaacgtaa
    39001 ggtgtcgcga atgcgtcgca aagagtgccg cgccagatag cccagcggga aaaatatcag
    39061 tgcgcaaaag acaataattt caatgatatc gctgatggtc atcatgatga gcgctccaca
    39121 gcatcatcta acagtcgcat tggttctggt attcgtcgcc agtggcgacc atcgtgctcg
    39181 gcattgatga ccggtttaga actttgcgct aaaggcagcg gcatgcccca ttgttctggg
    39241 gcaagcaggc gcatctgcac cagctcggca ctgatttgat catcttcaaa ccagaccatt
    39301 cggtttgaga aaatgtcgcc agtaggcaat gggaaaatat gattcaacgc ggtatccaga
    39361 tcgttaatcc gacagaatga gagaaacagc accagccgat taccgccaat ggtcatgata
    39421 tcgccggtac ggttagggcg acatagcgtc agggcttgct caacgcggat acccggtacc
    39481 ggacgtaggg caaccagaac gcctttaccg tgggcaggta atagagggtt attcatcatg
    39541 ttgttgacgg cattacagaa cacatcccac ttctggaaac cacgcagttt gagcggctgg
    39601 gtcattgaca gcaaggtagt gatatcttcc ggcacatagc gactaaactt ctgcccttgc
    39661 acgctttcga tcatcgtcag acagcgggag agtggcgcat tccacgggat aaccatattt
    39721 gcaccgcagg ccaataataa acgttcatcg gtggcgcgca ggctggcggt attttcccgt
    39781 acgaggattt tcatcgcact accgcgctgg cgacgcagag tatgaatgct gcgggccagt
    39841 ggctcgattt gcgcattttg ctgtaaagaa aagaccaccg tcgccgcctg agcggtacgg
    39901 gcttcattga acaggacttc gttattgttg aacagttgcc agtgttccga tagcggcggc
    39961 gcaccttcca gtacagcaac attactcaga atgcgttttt cgtcgctgcg tggttggatt
    40021 tccgcctcct ggtgttgaac taatgtccag caaccatttt gttgctgaac gctaagctgc
    40081 tgacgggcgc tgaccccttt ttcgttgcac cagaaagcaa tatccagcaa atgttggtcg
    40141 ccctgaaaac gcaaactggc aagaccaaaa agtgaccggt attcctcaag taacaatgaa
    40201 aattgtttat cgttattatt tccggaatta attaccaaaa gcgaacaatg gtttagccgg
    40261 ctccatttat tcattttatc caaccatgag cgaagccgct cggcaggaat gttttgccat
    40321 gcgttatttg cgcaaacaag aataaaaagg taattatggg gatcaataga acactgcaaa
    40381 tcacgggcca aatagtatag acctttttca tgatttagca ttgaaaataa tgttattttt
    40441 cccggccctt gagaatcatc taattgaaag attttcgctg gatcgctgtc catgctaatg
    40501 accgcgacgt gtgcggtttc agcctgggat gcaattgttt gattcgccag actgatagca
    40561 tcttcatggc gatcgacgtt aaaccaccag acgccgcctg ctggcatatg tcgcagctca
    40621 tcccataatg atgagatacc gatagagaat acagggtcca caatgtccct catgtcaaca
    40681 ttttgtctga ttatcagttt actagcgaaa gctaagaaat aaacctacca ttgaaattaa
    40741 aacttatcag atttagcatg tgaatcaatt tagcaggtca caagacatgt gattttagtc
    40801 ttttcgtttt taactttttc tgttgcgtaa cggaaagtca aaaagtgagc acattcccgt
    40861 ctcgccgtaa acaataaacc gacgaaatgc tcacagttag aattgtctca tgaacggtac
    40921 ggttatttca tagggatcaa gaaaaatgaa taacaatgaa ccagatactc tgcctgatcc
    40981 cgcgataggc tatatcttcc agaatgatat tttggcgtta aagcaggcat tttcactgcc
    41041 tgatattgat tatgccgata tttcccaacg cgaacagttg gccgcggcat taaaacgctg
    41101 gccgttgctg gcagagtttg cgcaacaaaa gtaggggatt ggtgaatggc cgtac
//
LOCUS       DAZFFE010000026        40843 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFFE010000026 DAZFFE010000000
VERSION     DAZFFE010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.026"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            382..2106
                     /locus_tag="ACHJ69_001655"
     CDS             382..2106
                     /locus_tag="ACHJ69_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO5014658.1"
                     /translation="MSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRCQLE
                     DQLEYQTLKARAEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLA
                     IPMGVGQRDSWSAKLRGLLASCQPAAIITGDEWLPLVNAATHNNNPELHVLSHAWFKA
                     LPEADVVLQRPVPNDIAYLQYTSGSTRFPRGVIITHHEVMANLRAISHDGIKLRPGDR
                     CVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPP
                     FGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPC
                     YGLAENALAVSFSDEACGVVVNEVDRDILEYQGKAVAPSAETRAVSTFVNCGKALPEH
                     GIEIRNEADIPVAERVIGHICISGPSLMSGYFGDQVSQDEIATTGWLDTGDLGYLLDG
                     YLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRIS
                     DEARRGQLIHALAARIQSEFGVTADIVLLPPHSIPRTSSGKPARAEAKKRYQKAYAAS
                     LHVQESLA"
     gene            2103..3017
                     /locus_tag="ACHJ69_001656"
     CDS             2103..3017
                     /locus_tag="ACHJ69_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO5014659.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGTPETQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVQQLVADVRCGSVRMVGIPQPLLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            3049..3297
                     /locus_tag="ACHJ69_001657"
     CDS             3049..3297
                     /locus_tag="ACHJ69_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO5014660.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            3297..4469
                     /locus_tag="ACHJ69_001658"
     CDS             3297..4469
                     /locus_tag="ACHJ69_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO5014661.1"
                     /translation="MGLYDKYARLAGERLQFSDYGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIREGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISTLADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDVN
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLVEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIASQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(4504..5583)
                     /gene="lptG"
                     /locus_tag="ACHJ69_001659"
     CDS             complement(4504..5583)
                     /gene="lptG"
                     /locus_tag="ACHJ69_001659"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO5014662.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDNDIIGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSTFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWHDRQH"
     gene            complement(5580..6650)
                     /gene="lptF"
                     /locus_tag="ACHJ69_001660"
     CDS             complement(5580..6650)
                     /gene="lptF"
                     /locus_tag="ACHJ69_001660"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO5014663.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYASVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFPR"
     gene            complement(6681..7241)
                     /locus_tag="ACHJ69_001661"
     CDS             complement(6681..7241)
                     /locus_tag="ACHJ69_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO5014664.1"
                     /translation="MPSRWIVALIIALLAPGAAAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLTGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFSQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGLGRVAWQLPEQSSTILVLNKAGRI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(7241..8077)
                     /locus_tag="ACHJ69_001662"
     CDS             complement(7241..8077)
                     /locus_tag="ACHJ69_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000984967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014665.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLKFWLDGVNRQELLRLIRKQAKGDELTTDERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGHFQDGFRNGKKNIVSFYGNLLRVYLSSPVWWLVNYSLRHSQ
                     LETVNGFIAYRQKQMYILKEIIAKPQLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALLISRFLAAINGLMGDKHDDMVADDMDNRRSYNAPVALDSDIRQRLKLLISRFPLYP
                     EQ"
     gene            complement(8077..9426)
                     /locus_tag="ACHJ69_001663"
     CDS             complement(8077..9426)
                     /locus_tag="ACHJ69_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO5014666.1"
                     /translation="MAVFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNDPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRQWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNSTGEKS"
     gene            complement(9472..10230)
                     /locus_tag="ACHJ69_001664"
     CDS             complement(9472..10230)
                     /locus_tag="ACHJ69_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014667.1"
                     /translation="MDALQTQTVKSTTAPQPNYIPGLIAVVGCDGTGKSTLTNDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISRF
                     LAQRERRLYQKMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(10262..10975)
                     /gene="yghS"
                     /locus_tag="ACHJ69_001665"
     CDS             complement(10262..10975)
                     /gene="yghS"
                     /locus_tag="ACHJ69_001665"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO5014668.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELASRMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKNQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            11150..11842
                     /gene="yghT"
                     /locus_tag="ACHJ69_001666"
     CDS             11150..11842
                     /gene="yghT"
                     /locus_tag="ACHJ69_001666"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO5014669.1"
                     /translation="MQSIIPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEKVLVDAEK
                     AITDFMTARGYH"
     gene            complement(11891..13390)
                     /gene="pitB"
                     /locus_tag="ACHJ69_001667"
     CDS             complement(11891..13390)
                     /gene="pitB"
                     /locus_tag="ACHJ69_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO5014670.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLNGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLITMEPPLPATSTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(13682..15541)
                     /gene="gss"
                     /locus_tag="ACHJ69_001668"
     CDS             complement(13682..15541)
                     /gene="gss"
                     /locus_tag="ACHJ69_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO5014671.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHTPLPQGQQ
                     WTRELEMVVENGGYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALQQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDQTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            15746..16612
                     /gene="yghU"
                     /locus_tag="ACHJ69_001669"
     CDS             15746..16612
                     /gene="yghU"
                     /locus_tag="ACHJ69_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO5014672.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFASINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTEAMSWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERLAVKRGRIVNRINGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(16745..16993)
                     /gene="hybG"
                     /locus_tag="ACHJ69_001670"
     CDS             complement(16745..16993)
                     /gene="hybG"
                     /locus_tag="ACHJ69_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO5014673.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(17006..17347)
                     /gene="hypA"
                     /locus_tag="ACHJ69_001671"
     CDS             complement(17006..17347)
                     /gene="hypA"
                     /locus_tag="ACHJ69_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO5014674.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(17340..17828)
                     /gene="hybE"
                     /locus_tag="ACHJ69_001672"
     CDS             complement(17340..17828)
                     /gene="hybE"
                     /locus_tag="ACHJ69_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO5014675.1"
                     /translation="MTEEIAGFQTSPKAQVQVAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(17821..18315)
                     /gene="hybD"
                     /locus_tag="ACHJ69_001673"
     CDS             complement(17821..18315)
                     /gene="hybD"
                     /locus_tag="ACHJ69_001673"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO5014676.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     ATHD"
     gene            complement(18315..20018)
                     /gene="hybC"
                     /locus_tag="ACHJ69_001674"
     CDS             complement(18315..20018)
                     /gene="hybC"
                     /locus_tag="ACHJ69_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO5014677.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPAKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(20015..21193)
                     /gene="hybB"
                     /locus_tag="ACHJ69_001675"
     CDS             complement(20015..21193)
                     /gene="hybB"
                     /locus_tag="ACHJ69_001675"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO5014678.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(21183..22169)
                     /gene="hybA"
                     /locus_tag="ACHJ69_001676"
     CDS             complement(21183..22169)
                     /gene="hybA"
                     /locus_tag="ACHJ69_001676"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO5014679.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSEHVQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(22172..23290)
                     /gene="hybO"
                     /locus_tag="ACHJ69_001677"
     CDS             complement(22172..23290)
                     /gene="hybO"
                     /locus_tag="ACHJ69_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO5014680.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMVESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(23479..23766)
                     /gene="yghW"
                     /locus_tag="ACHJ69_001678"
     CDS             complement(23479..23766)
                     /gene="yghW"
                     /locus_tag="ACHJ69_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO5014681.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(23885..24772)
                     /gene="yghX"
                     /locus_tag="ACHJ69_001679"
     CDS             complement(23885..24772)
                     /gene="yghX"
                     /locus_tag="ACHJ69_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO5014682.1"
                     /translation="MPRLTAKAFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVTRRVAKEGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGISGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDSRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            24929..25969
                     /gene="gpr"
                     /locus_tag="ACHJ69_001680"
     CDS             24929..25969
                     /gene="gpr"
                     /locus_tag="ACHJ69_001680"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO5014683.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLREWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLHEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(26009..26503)
                     /gene="yqhA"
                     /locus_tag="ACHJ69_001681"
     CDS             complement(26009..26503)
                     /gene="yqhA"
                     /locus_tag="ACHJ69_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO5014684.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQVIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            26694..27578
                     /gene="yghA"
                     /locus_tag="ACHJ69_001682"
     CDS             26694..27578
                     /gene="yghA"
                     /locus_tag="ACHJ69_001682"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO5014685.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(27617..28042)
                     /gene="exbD"
                     /locus_tag="ACHJ69_001683"
     CDS             complement(27617..28042)
                     /gene="exbD"
                     /locus_tag="ACHJ69_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO5014686.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(28049..28783)
                     /gene="exbB"
                     /locus_tag="ACHJ69_001684"
     CDS             complement(28049..28783)
                     /gene="exbB"
                     /locus_tag="ACHJ69_001684"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO5014687.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            29035..30222
                     /gene="metC"
                     /locus_tag="ACHJ69_001685"
     CDS             29035..30222
                     /gene="metC"
                     /locus_tag="ACHJ69_001685"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO5014688.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDCVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALGFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            30362..31021
                     /gene="yghB"
                     /locus_tag="ACHJ69_001686"
     CDS             30362..31021
                     /gene="yghB"
                     /locus_tag="ACHJ69_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO5014689.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYSLSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(31061..31960)
                     /gene="yqhC"
                     /locus_tag="ACHJ69_001687"
     CDS             complement(31061..31960)
                     /gene="yqhC"
                     /locus_tag="ACHJ69_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO5014690.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            32154..33317
                     /gene="yqhD"
                     /locus_tag="ACHJ69_001688"
     CDS             32154..33317
                     /gene="yqhD"
                     /locus_tag="ACHJ69_001688"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO5014691.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPSRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            33422..34249
                     /gene="dkgA"
                     /locus_tag="ACHJ69_001689"
     CDS             33422..34249
                     /gene="dkgA"
                     /locus_tag="ACHJ69_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO5014692.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            34449..35375
                     /gene="yqhG"
                     /locus_tag="ACHJ69_001690"
     CDS             34449..35375
                     /gene="yqhG"
                     /locus_tag="ACHJ69_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO5014693.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTSQTLKMVAENGRWVIGDIVSNHGSVLQAVNSENEKTLA
                     AIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYYNPICFGEESLFSRVDEIRVLEKTTDSARIHVRFTLTNGNNEEQELILQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            35426..35683
                     /gene="yqhH"
                     /locus_tag="ACHJ69_001691"
     CDS             35426..35683
                     /gene="yqhH"
                     /locus_tag="ACHJ69_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO5014694.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKINQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(35725..37944)
                     /gene="ygiQ"
                     /locus_tag="ACHJ69_001692"
                     /pseudo
     CDS             complement(35725..37944)
                     /gene="ygiQ"
                     /locus_tag="ACHJ69_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
     gene            38196..38945
                     /locus_tag="ACHJ69_001693"
     CDS             38196..38945
                     /locus_tag="ACHJ69_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO5014695.1"
                     /translation="MEQVITKRRYYDIGLQIEELLYSGVFKAGERLPSERELSERFNT
                     SRTTIREAIIMLELKGVLNVKQGSGIFFVDSTDKLNQKSLMPYSEIGPFELLQARQVI
                     ESNITGFAASQISFNELQELKKIIGLQEKAIAAESDKFEDLDHRFHSIIAEATQNRVL
                     IKQAAELWRAVRTENPRWKKLNYKYLHEKHLRLQWLEDHRAIFLALQQKDSELAREAS
                     WRHLENSKNELIKIFKQDASISDFDDFFFAR"
     gene            39270..40742
                     /locus_tag="ACHJ69_001694"
     CDS             39270..40742
                     /locus_tag="ACHJ69_001694"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO5014696.1"
                     /translation="MSGNESIDAAHYDQVTTWPRDELQADIVHIGFGAFHRGHQAVYT
                     DLTNQLSDTHWGIFEINLFGDAQLIENLNAQNGLFSVVETSASQSTSRLVRSVTGGIH
                     TPVDGIAAAIQKLTEPQVKIVSLTITEKGYCLDSQTRSLDLTNGLIKHDLQNPDAPQS
                     AIGLIVCALQQRKAAGLAAFSVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENV
                     SFPGTMVDRIVPAMTESQFALLETKTGYADPCGIVCESFRQWVIEDNFVRGRPEWDKA
                     SAMFVSNVQPYEEMKLRMLNGSHSFLAYNGSLAGYEFIWQCMEDANFRSITRQLMINE
                     QARTLNPDLNINIQEYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQ
                     KQNNAVLSLLVAGWLHYVIDAVEKSQSVADPMNDQLQALIKEQQDAWQQALALLHLSA
                     IFGDLSNHQPFINEIKIAFANIKNKGIKATISQLLSDEQK"
     gene            40739..>40843
                     /locus_tag="ACHJ69_001695"
     CDS             40739..>40843
                     /locus_tag="ACHJ69_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate oxidoreductase"
                     /protein_id="HFO5014697.1"
                     /translation="MKTLICQQPGVMEYVEKDIPTPADNEVLLKIKAVG"
BASE COUNT        10394 a        10645 c        10037 g         9767 t
ORIGIN      
        1 tcgcgttcca tagcttagtg gataacaaat aattaacggt gaaaaacaag ttgcaacagc
       61 aacgaacggc atcttaataa tcaacccaat gaatacaaac aaagaataaa tagtatttat
      121 taatgattac gataaggtca gagactacac agaagcgcca caatgccttt tgtcgattta
      181 aggcgaccgg acaaggctcc ttcttaatat attcacaaat taaatatcat tcacaataaa
      241 aacaaaaaag ccaaacctat tatttaaatc aatgttcatt ttcacaccga tgaaaatact
      301 atctcattta tcttttatgc ctatataatc caccgcatta ttgtcatttt atttttgata
      361 tccaattttt tcgtggtgta tatgtctaat aaaatcttta cgcattccct acctatgcgc
      421 tatgccgatt ttccaacgct ggttgatgct ttggactacg ccgctctgag tagcgccgga
      481 atgaattttt atgacagacg ttgccaactt gaagatcaac tggaatatca gacgttaaaa
      541 gcgcgtgccg aagctggtgc gaaaaggttg ttatcgctga acctgaaaaa aggcgatcgc
      601 gtggcgctga ttgccgaaac gagtagcggg tttgtagagg ctttttttgc ctgccagtat
      661 gccggcttag tcgccgtccc gttggcgatt ccaatgggcg taggtcagcg ggattcctgg
      721 agcgccaaac tgcggggttt actggcaagt tgccagcccg cagccattat cactggtgat
      781 gagtggttgc cactggtcaa tgccgcgacg cataacaaca accccgaatt acatgtttta
      841 agccacgcct ggtttaaggc attaccggaa gccgatgttg tgctccagcg tccagttcca
      901 aacgatatcg cctacctcca gtacacctcc ggcagcaccc gttttccccg tggcgtcatt
      961 atcacccatc acgaagtgat ggctaatcta cgtgctataa gccacgatgg gattaaatta
     1021 cgccctggcg accgctgcgt ctcctggctg cctttctacc atgatatggg actggtcggc
     1081 tttctcctga cccccgtcgc cacgcagctt tcagtagatt atttgcgcac tcaggatttt
     1141 gccatgcgtc ctctgcaatg gcttaaattg atcagtaaaa atcgtggcac cgtttccgtt
     1201 gcaccgccgt ttggctatga attgtgccag cgccgcgtga atgaaaaaga tctcgctgaa
     1261 ctggatcttt cctgctggcg cgtcgctggt attggcgcag aacccatatc cgcagaacaa
     1321 ctccatcaat tcgctgaatg tttccgtcag gttaactttg acgataaaac tttcatgccg
     1381 tgctacggac tggcagaaaa tgcgctggct gtcagcttct ctgatgaagc ctgcggggtt
     1441 gtggttaacg aagtggatcg cgacatcctc gaataccagg gtaaagccgt cgcgccgagt
     1501 gctgaaacac gcgccgtatc gactttcgtc aactgcggta aagcgttgcc ggaacatggc
     1561 attgaaatcc gcaatgaagc agatatacct gtcgcggaac gtgtgatagg ccatatttgc
     1621 atctccggcc ccagcctgat gagcgggtac tttggcgacc aggtttcgca agacgagatt
     1681 gccacgacgg gctggttaga caccggagac cttggttatc tgctggacgg ttatctgtat
     1741 gtcaccggac gcattaaaga tctgattatt attcgtggcc gtaatatctg gccgcaggat
     1801 attgaataca ttgcggaaca ggagccggaa attcattctg gcgatgcgat tgcttttgtt
     1861 accgcccagg aaaaaatcat tttgcagatc cagtgtcgga tcagcgacga agcacgtcgc
     1921 gggcagctta tccacgcgct ggcagctcgg atccaaagcg aatttggcgt taccgcggat
     1981 atcgttctgt tgccgcccca cagtattccc cgaacatcct ccggcaagcc tgcccgtgcg
     2041 gaagcgaaaa aacgttatca gaaggcttat gctgccagtc ttcatgtgca ggaatccctg
     2101 gcatgaatca aactgtcgcg gtgacgggcg ctaccgggtt tatcggtaaa tatattattg
     2161 ataacctgct cgcccgcggc tttcacgttc gcgcattgac gcgtactgcc cgcgctcacg
     2221 tcaatgataa tctcacttgg gtgcgcggtt cgctggaaga tacacattcg cttagcgaac
     2281 tggttgccgg agccagcgcg gtggtccatt gcgccgggca agtgcgcggg cacaaagaag
     2341 agatttttac ccgctgcaac gttgacggca gcctgcgcct gatgcaagcg gcaaaagaga
     2401 gcggcttttg ccaacgtttt ctgtttatct cttcgctggc ggcgcgccat cccgaactct
     2461 cctggtacgc aaattccaaa cacgtcgccg aacaacggct gactgcaatg gctgacgaaa
     2521 ttacgttggg cgtttttcgc ccgacagccg tgtatggtcc cggcgataaa gagttaaaac
     2581 cgctgtttga ctggatgctg cgcggtctgc tgccacgact tggcacgcca gagacacagc
     2641 tctcttttct tcacgtcacc gattttgcgc aagcagtggg tcagtggtta agcgccgaaa
     2701 ctgtacagac gcaaacctat gaattatgcg atggcgtcgc cggaggctat gactggcaac
     2761 gtgtccaaca gcttgttgcc gatgtccgct gtggttccgt gcggatggtc ggtattcctc
     2821 agccgctact cacctgcctt gcggatatca gtaccgcgtt gagccgtctg gcgggtaaag
     2881 aaccgatgct gactcgttcg aaaatccgtg aattaaccca tgccgactgg tcggcaagta
     2941 ataaccgtat ttctgaagat attaattggt ttcccgggat tagcctggaa cacgcattac
     3001 gcaacgggct attttgattg tcttgtttaa aggtgtgtta atgtttctat ggtaaatcgt
     3061 gaaatagtaa tggattacat cctttcctgt ttacaggatt tggtcgaaaa cggtgtggaa
     3121 attaagcccg acagcgatct cgtcaacgat cttggcctgg agtcgatcaa agtgatggat
     3181 ctcttaatga tgctggaaga cagattcgat atttccattc ctattaatat tctgctggat
     3241 gtcaaaaccc ctgcgcaatt aatggaaact ctacttccat ggctggagaa taaataatgg
     3301 ggctatacga taaatatgcg cgccttgctg gcgagcgcct gcaatttagt gactacgggt
     3361 taacaccatt tggcacctgt attgatgaag tgtattccgc taccgaaggc cggatcggca
     3421 ataaaaaagt gattctggcc gggaccaata attatctcgg tttaaccttt aatcatgatg
     3481 ctatccgcga aggtcaggca gcgttagcgg cccaaggcac cggcaccacc ggttctcgta
     3541 tggcaaacgg cagttatgcg ccgcatctgg cgttagagaa agagattgcc gagtttttca
     3601 atcgccctac cgctatcgtt ttttcaaccg gttataccgc taatttaggc gtcatcagta
     3661 cacttgccga ccataacgcc gtggtgttac tggatgccga cagccacgcc agtatttatg
     3721 acgcctgttc tctcggcggc gcggagatta ttcgtttccg ccataatgac gtcaacgatc
     3781 tggaacggcg catggtccgc ctcggtgaac gggcgaaaga ggcgattatt atcgttgaag
     3841 gcatctacag tatgctcggt gacgttgcgc cgctggcaga aattgttgat atcaaacgtc
     3901 gtctcggcgg atatttaatt gtcgatgaag cacattcgtt tggcgtgtta ggcgcaacgg
     3961 ggcgcggact ggttgaagcc gttggcgttg aagacgacgt ggatattatc gtcggcacct
     4021 tcagtaaaag tctggcctcg attggcggtt ttgctgtcgg ttcggaagcg atggaagtgc
     4081 tgcgctacgg cagccgcccg tatattttta ccgcctctcc ttctccgtcc tgcattgcga
     4141 ccgtgcgctc gtcgttaaga accattgcct cacaaccaga gctgcggcaa aagctgatgg
     4201 ataacgccaa ccatctgtat gacggtctgc aaaaactggg ttacgagtta agttcacata
     4261 ttagcccggt agtgccagta ataatcggtt cgaaagagga aggtttacgc atctggcgcg
     4321 agcttatttc gctgggcgtt tatgtgaatc ttatcctgcc acctgccgcg cctgctggca
     4381 tcacgctgct gcgctgtagc gtaaacgctg cccacagtcg tgagcaaatc gatgccatca
     4441 ttcaggcatt tgctactctg aagcagtaat gtgaacccgc cgtggtgttg cgccacggcg
     4501 ggtttaatgt tgtctgtcat gccaatacac caggctcaac gccaccagca caaacgctac
     4561 cggtaaaccg agcgcggtaa caggtgcgct caacgcaaat agcaatccca gattgaccat
     4621 gatttgatag ctgatccagg tgagtaaacc aacgatgacg cctacagcga gacggctacc
     4681 catccctggc gagcgcgggg cactaaaggt aaacggcaca gccagcaaaa tcatcgccag
     4741 agtcaagatg ggttgcccca gtttttccca cagtgcaagg cggtattcgc tgctgggttg
     4801 cccggtttcc tgcaaataat ggatgtaatg attaagctga cggacagaaa aagtattccc
     4861 cggcatcgat aactcttcaa gattcatact ggtgaaggtc gattgccagg caagattatc
     4921 agatgtttcc agcgtttctt tgccattcag ccatttttta tgattcacac catgcaggat
     4981 ccacgtttta tcgtcttcaa tggtggcact gcgcgcataa atgtaggatt cgagcgagag
     5041 atcgtcgcgg taatgaaaaa tctccacgcc caccggctgg ccctgctcgt tcagcgattt
     5101 caccgtcaca aattcattac cgcgcctggc ccaaagcata ttgccgataa tgtcattgtc
     5161 ctcccccaac gcggtggcag tcgatttgat ttgcagcgcc tgttgctgca atggtgacgc
     5221 cacccactca tcaatcgcgc ctaacgaaac agtccacagt atccctgcaa ccagcgccac
     5281 cagtgcaatg cggaagatag aaaaccccgt gctgcgaatg gcggtaagct cactgttttt
     5341 cgataactgc ccgagaccaa caatcccccc caataacgcg ataaacggcg aaagttcgac
     5401 cagtgtgcgc ggtaaggtca tcagcaccac cagtactgcc tgagtccagc gataaccgcc
     5461 cggactgaca tcatccagtt cgttaatcag gttgaaggtg gtaaaaagcg gcagcaatag
     5521 ccctgcggcg gcggcaaaac cgagaaagag gtgacggatt aaatagcgac tgaaaacatt
     5581 catcggggaa atttctgcag caaggagaag tcgcgtgcga ccagcatcaa caggcccatt
     5641 agcatgagtc cgggcactaa ccacaaacca ggaatgaggg gaatcgcgcc gttagccacc
     5701 agcgtacgac agacgtcgcc gccataaaaa atggcaacaa acagcaacgt cagtggcagt
     5761 agcgttgaaa atcgcccctg ccgcggtttt acccgactta atgaaatggc cagcagcgcc
     5821 atcaacaatg ctgtcaggcc acggctttgt cgccattgca gttcggcatg atcggctgga
     5881 aagacggagc gtgccagctc cgtgaccgat tttgctttgc gtttgacgtt agggctttga
     5941 tccagcggat tgagatgcaa ttgcagatta cggtagatct gttcgttgtc gtcacgcccc
     6001 tgatgatcga gaagataggc ggtaccgtta tgcaacataa cggtcggttt ctcgggtgat
     6061 gggtcaacca catcaaccga acgggcgcgg aaaatgcggg ttcgattggc agtagaagta
     6121 taaatcagcg cgtcagtcag gcgattatta tcctgatcaa ccgtctgcga aaggatcatt
     6181 cgtccgttat cgttagtgtt aaatttcttt gcccgcaact gacgaacatc cagctccgac
     6241 tgcgattgtt gctccagttg ataaatctgc gcataagccc agggacgacc atacatcgac
     6301 agaagggtga caaaaatact taacggtatc gccagataaa gaacggcttt gtacaaacgt
     6361 cccggactgc cccccgccgc ggagatagcg gtaatttccg agtcggtata catttgccct
     6421 agcgtcacgc caactgaagc atatagtcca acaggtaaca gcatctccag tgcaatcagc
     6481 actttgtaga aaacgatatc cagcacaaca tcaagagcta atgtcccatt tgccgcttcg
     6541 gtcagataac gctgtgcgga gtagctggcg aaaataaaga tcagaaaacc gacgataatc
     6601 agcaccagac ggcgcgtacc acgcatgatg tagtgttcaa ccagcttcat cttgctaacg
     6661 ccctcaatcc tttgaatttt ttattcattg atcagtttct gcgccagcgc aatgacatga
     6721 tctacctctt ccggtgtcag gctgccgtcc tttgcccact gaattcgccc ggctttattc
     6781 aataccagaa tggttgaact ctgctccggc aattgccagg caacgcgtcc cagaccattg
     6841 ccgtcaataa cgaattgcgc ccacggatag cggcgtttat ttttttcgat tttgccaacg
     6901 acaaaatagc cagtaccaaa gatggcatca tcggtgttca caatagtggt gggctgaaaa
     6961 cgatcctgcg aaaagtttgc cgcttcaacc gctttaatta acagtgagtt ctttttcttc
     7021 gcagacttac gtccggcgat atactgaata atgcggactt ttcccgtcaa ttgtgtgctg
     7081 ttccactttt ggtagtgaat ttcgtcgtcg ctattcagaa gcagctcgcc gccttcctgg
     7141 atataaacag gagtaaccgt tttgccagtg acgaaattgt gtgccgccgc gcctggcgct
     7201 aagagtgcga tgatcagagc gacaatccaa cgagatggca ttattgctcc ggatatagtg
     7261 ggaaacgcga aataagcagt tttaaacgct ggcgaatatc gctatccagc gccaccggtg
     7321 cattatatga ccgacggtta tccatatcat ccgccaccat gtcgtcgtgc ttatccccca
     7381 tcagaccatt aatggcggcg agaaaacggg aaatcagtaa cgcctcttta ttttcaggat
     7441 caagcgatct taaggtgtcg taataagaaa cctgctggct gatgattttt cgcacatcat
     7501 gaaattccct gccagttagt tgcggtttag caataatctc tttcaggatg tacatttgct
     7561 tctggcggta ggcgataaaa ccgttcacgg tttctaactg gctatggcgc aatgaatagt
     7621 tcaccagcca ccagacaggg gagctcaaat aaacacgtaa caggttaccg taaaacgaca
     7681 caatattttt cttaccgtta cgaaagccat cctgaaaatg cccaagaaaa acggtcgttt
     7741 tgccgaataa aaatccggcc tgatgcttct ttaaatacag ccgttgcgca aaacgcagat
     7801 gcttgtagcg cgctcgcatg tatttaaact gtttgcgctc gtctgtcgtc agctcatcgc
     7861 ctttcgcctg tttgcggatt aaacgcagta actcctgacg attaacacca tccagccaga
     7921 atttcaggca taacgcgtaa ttatcatgga tgatttcggt ggtacaactt agtgcgatag
     7981 tcggcggcaa actgacctgt gcatcaacaa catcatcttc ttcaatcgca acaaatagac
     8041 gctcgctcgc ctcaagcggg aaatattttc tcatcatcag gatttctctc cggttgagtt
     8101 ataaagcaag gaatggcgct gaaaaaatgc ctttaaggtc ggaattagcg agagcaccac
     8161 atccagacat tgcccaccga agtaagcaaa tgcagccccc agcacgccaa acagatgaca
     8221 gaacatgaac agcaatacga tgtagccgat tgaggcgatg gtttgcgcca cgagaaaagc
     8281 gcgctgtttt cccgccatca atagcaatga ctcctgtggg aagcccagca tcgaaatcac
     8341 aattgcgccc agcatcacct gaatcaggtc atacgcttcg agatacttaa cgccaaacac
     8401 cagcgaaatg agcggcttgc cgacgatcaa caccgcgagc gccaccagaa taccgattcc
     8461 gcccgccagt aaaccggact tcacgcctaa caaccactgt ctggtggtgc gtggatctaa
     8521 acgcatcacc tctggataaa aacttttgcc cagtaaaccc gctggcgttc cggcggcatc
     8581 gaagaatgtc atggcgattt taaataaccc ggcagcagcg ggtcctaaca cgatccccac
     8641 caacactgtg ctgcacgagt tacgcgccga ccagatggag tgggcaatgt tggttgacca
     8701 gacaaaactc cacgcgcctt taatgtgtcg ggcagactca aacagattca atttgaaggc
     8761 gttatggata tttctgcggc gtaattcgcg cgcggcaaac caccagtaca tggtgccgcc
     8821 aaccagattc gacacgtacc aggcaataac aaaacccgca aaaccaaagt caaaatacca
     8881 ggctacgacg ctccccgctg cgcgcagaaa aggtttcgtc gcctgctgta cagcaatcaa
     8941 atcaaagcga tctaccgcac gcagaatgcc ggtcggcgtg gaggaagcca ttgaaggaat
     9001 gagcgtgcaa tagagcgccg ccagccaaaa gctttggtca tccagaccta atgaatggga
     9061 aaggaatggc agtaaggcaa tgccgccgac aatcgccacc gcgccgctga cgatatccag
     9121 cgagaaggaa aatgagacaa cattgcgaaa ttgctgcgga tcattgttgg tcaaagctgg
     9181 cgttccgtac tgaaccacca gctgccatgt ctgaaactta ataaaatcgc tgatcgactt
     9241 ggcgtacgat tgcacaatta ccagtacgcc aaacatggcg ggcgtcatcc ctttaccggc
     9301 acacgagagc gccaacagcc ccagcaaagc gctcacaaca ttactggagc ctaaccaggc
     9361 gctattgcga ataatggtgc gaaacgcgcc atctgcaaac caatgtttga tgttaaaaac
     9421 cgccaattca gcctgacctt atcgttgata gtaaaaagta tcccgccagc cttaagttaa
     9481 acttcggcgg tcagaaacga tggcaaccag agaaaccgcc ttctgtgcct gttccagcac
     9541 ctcgctgtaa ggcgctctgg aatcaatctc aagaattttt gtgccgttat agccaatcgt
     9601 cgacataaca ccgattttgt cctgcagctc ggcatagtca tggtcaggct tgcgggagat
     9661 ggcagtatca atatcaatcc ccaagcgaat gattaattca ggacggtatt gcgccatttt
     9721 ttggtataaa cgccgttcac gctgcgccag aaacctgctg attttcccgg tcgcacgttc
     9781 gacgccaatc cccggtccgt cataataaaa gcccgaaatt tcagcctgcg ggaagcgatc
     9841 gctgaccacc agaacgccac tttgcgccag tcgctgaacc ttgcgcagat tcgccattct
     9901 tcgcagcgag aagcagtaca taatcaccgc cgcccatagc gccggagatt tggttttcat
     9961 gctttgggtt ttcgaggatt tggccgccag tcgccgttcg agccagacac ccaccaacgg
    10021 caatcgtttg attttgtcgc cgtcttcgcc ggagagcagc cccagatagc gccgctcggt
    10081 ttgccaatgt tgttgcagcg atttcaccag gtcatttgtc agtgtggatt taccggtgcc
    10141 atcacacccg accaccgcaa tcagccccgg aatgtagttg ggctgcggcg cggttgtact
    10201 cttaacagtt tgtgtttgta gtgcatccat tcgtttaatc atcccctatc cattttggtg
    10261 atcaggaaag cgcggcgtga attgcgcgta gtgacgcttg cagaatttca tcggctggtt
    10321 gccgaccatc cagttcaagg atcctggcgc cattgaatgt cagttgcggc gtaacggcga
    10381 ttttttcctg taacgctgcc agttggtggt caggtttacg cgcaaacgcg gtttgttcat
    10441 caatgccaag acgaatcaac aatacaggca aataagacgc catccattgg tacagcttca
    10501 gctcacgctg ccttaacatt tttatccaac cgttaccgcc cgtggttttt gccaattgcg
    10561 ggccatcaaa gcggaaaccc ggcacttcaa cctgcgggta gcggtcggtg atgagcagat
    10621 agccttgctg gtttttacac aacattttgc gaaacttgta cgcccgccag caggaaagca
    10681 gatagataac cagcgcagta atattgccag gcggtgttga gggcttttcg tgcacatgtg
    10741 ccgctttact tcgcaaataa cgcccaaaag gtgcgccaat aacaggtagc tgtgaaatcc
    10801 attcgccaat tcgcccggac gattgcccga gataaatgtg ttctgttggc attcttgatg
    10861 ccagttcatt taccaggctt gccgtgaggg tcgatttacc tgaaccatca catcctacaa
    10921 tggcaataac acgcactggc gtggaattaa ttattgacat attacgttga ttcacgaaaa
    10981 acccggcagc aaataatgta ttgaatatta cgtggtccgt gctcagaata tccgttcaac
    11041 cttattgtga caatggttaa ttgtgacatt gacctgaatt acgctttcat caaaaacata
    11101 ttaaccaaat aaatattttg gatggatatt taaatttaag gatatattca tgcagtcaat
    11161 aatacctcca ttaattgccg ttattggtag cgatggttca ggcaagtcaa cggtgtgtga
    11221 acatcttatt accgttgtcg aaaaatatgg tgctgccgaa agggttcatt taggaaaaca
    11281 ggccggaaat gtcggtcgtg cagtgacaaa attaccgttg atgggaaaat ccttacataa
    11341 aacaattgaa cgaaatcagg taaaaacagc aaaaaaattg cctggaccag ttccggcgct
    11401 ggtaattaca gcgtttgtcg cccgtcgctt actgcgcttt cgtcatatgc ttgcctgtcg
    11461 tcgtcgcggg ttaattgttt taaccgaccg ttatccgcag gaccagattc ctggcgctta
    11521 tgatggtacg gtgtttccgc ctaacgttga aggtggtcgt tttgtctcat ggctggcaag
    11581 tcaggaacgt aaagcatttc actggatggc gagccacaag cccgatctgg tcatcaaact
    11641 caatgttgac cttgaagttg cctgcgcgcg taaacccgac cataaacggg aatcgctggc
    11701 gaggaagatt gccataacgc cacagttaac ctttggtggt gcacaactgg ttgatatcga
    11761 cgccaatcag ccactggaaa aggtgctggt tgatgcagaa aaagcgatta cggattttat
    11821 gaccgcgcgt ggttatcact agtgaaaaat ggaaatgccc gatcgccaag accgggcatt
    11881 ttcaggaagg ttaaatcaac tgcaatgcta tccagtacaa cccaccagaa agaaaaatcg
    11941 ccgccggtaa agtaaatacc cacgccatca ggatgctggt taccgtttta cgctgtaacc
    12001 caccgccgtc caccaccatc gttcctgcta cggcagatga gagtacgtgt gttgtggaga
    12061 cgggcatccc aatataactg gcaaggccga tagacactgc cgccgtcatt tgtgccgcca
    12121 tgccttgcgc atacgtcatg ccgcgcttac caatcttctc accgatggtc atcgctacac
    12181 gacgccagcc aatcatggtg ccgatgccga gcgccagtgc taccgccatg atgatccata
    12241 ccggtgcgta ctcaatggtg cttaacatat cgctgcgcag ttttttcagc aggttctggt
    12301 cttctttact gacgcctggc agtttcgcca gcttcgcgga ggtatcagag atgcacagca
    12361 taatgcggcg cagctggctg cgctgactca cgcttaacgg ctcgtaactt tccatattgc
    12421 ctggcagcat cgttttaacg cgcgcaatcg catcaaaggt atttgccgga tgacagtgaa
    12481 actctgttac ttgcgtgcca tcagtcgatg tcgcaggcaa tggaggttcc atcgtaatca
    12541 acttctgcgg cagttcagga tgctgttgca ggtagtgttc gaagttggtg acggcatcgc
    12601 gggtacgggt aatttcatag ccggacgcat tcatattgac gacaaacccg gcgggagcaa
    12661 tccccaccag caccagcatc accagaccaa ttcctttctg cccgtcgttc gcgccgtgcg
    12721 aaaacgccac gcccgcagcg gaaacaatga gggcaatacg cgtccagaat ggcggcttac
    12781 gtttgccttt tttctttttg cgatcttccg gaatgcggtg aatacggtca cgctttttcg
    12841 tcccactcca gaaacggcgt agcagaaaaa ttagcccccc cgcaatcacc agccccacga
    12901 tgggagagac aatgagtgaa gagaaaatct tggtgacttc gcgcaggttt aacgcatcca
    12961 tcaccgacga accatttaac agcgcgttgg ttaaaccaat gccgataatc gcaccaatca
    13021 aagtgtgcga actggaggcc ggtaaaccaa agaaccacgt tcccagattc cagataatcg
    13081 ccgccagcag catggaaaag accatcgcca gaccgtgagt tgaccccata ttcagcaaca
    13141 aatcggttgg caacatatgg acgatggcat aggcaacgct aagcccgcca agcaatacgc
    13201 caaaaaagtt aaaaaatgcc gccatgacca cagcaagctg tggttgcatg gcacgggtat
    13261 aaataacggt tgccaccgca ttcgccgtgt catgaaaacc attgattgct tcatagaaca
    13321 acacaaatgc cagagcaagt aataacaaaa gccccgtata tatatcaagg ccaacaaata
    13381 aatttagcat atattagatt acgccatttt gaatttacga acggaggcat tatcagtgac
    13441 tttcacggcg tgggcaaagt gaaatatcat tttttaacga taaaaagcgt gttatttctt
    13501 gtgtatcaga caataagcct tcattcaaaa cctttaaaaa taatcaaaac acaggtattc
    13561 cacttgttaa tatattcata tatgaaatga ataaatattg aaatatataa atattgaata
    13621 ttttgattaa tccccgtact gattattctt cataatcagt acggggttgc tacaacatgt
    13681 attactcttt caccacaatt aacggttcaa tatcactctc ttttttgatg accagtgatt
    13741 catcaccgcg caaacacgtc ccaccgtagt tgccgccaac ggtgaaagta catacctgaa
    13801 tgtatttacc gtccactttc ggcaaacacc acagttgctg gtaaatattt ttctgttcgg
    13861 caaatttacc actggtctga tcaagcacct cttcatgatg gctgaccagg tcaatgttgc
    13921 taccacagcg cccggcaatc ggtttcacgg cgtaaccggt tttcaccagt tcatcattaa
    13981 cagtgaaatc ggtatccagc agataacgat gatgcgggaa cagcgaccag agaattggca
    14041 gaatcgcttt gttgccaggg atcaccgtcc aaagcggttc aaagaccagc acttccggac
    14101 gtagcaatac gtcgataaga cgcacttcat tttgcgggtg accagtgcga atcgggaccg
    14161 cagcaaattc acggtcgcta acttcacgaa tctgatcaaa cgccgtttcc cacgcccagg
    14221 tcttccacac gcagttaacc agtcgccctt ccccatcaat cagttgcccg gcagcatccc
    14281 agcccagttc atctagcccg cgcaagatac gcgtttcaaa gcccgcctgt tgcagcgcct
    14341 gctccataaa ctgagcgtga tagttctctt caatatcttt gtcctgcatg atatggacaa
    14401 acggacgcgc gcgactgtgt ttccaggcac cagccagttc gttaatcagc ccttccgcag
    14461 gattgaagcc gttgccttta tagccctgct ccgcccaacg ttcgaggatc aagcccgctt
    14521 cggtatgaca ggaggcagaa tcggcgttgt attcgtagac tttcaggcca cgttcatcca
    14581 tgcagaaatc catacgaccg gtgatcatat ggtgacggcg acgctgccag gagagacgca
    14641 aacgtggcca gaggattttc ggaatgtcga acaacgccag caggttgtca tctttcagca
    14701 ccttgtcggt ggcgtgaaga tacatcaggt gcagctcgtt ggtggcttta atcagctcct
    14761 gctcggcgct ctcggtaatg gtgtagtaat gatacggatc ctgattgatc acctgaccgt
    14821 tggcctgtac ataggcgttt tgcagcggat ctttttcatc cagccattta ccgtcaaact
    14881 ggcctttgtt ttccagacgc gccccgctga ttttcagcag ttcgcctgca atttccggct
    14941 gtggcaagct gtattcggta tcttccgtct ggatcatcca gcccagaata gtggtgtcat
    15001 cgaaggtgtc tttcagggta tagccgccgt tttcgaccac catctccagc tcgcgtgtcc
    15061 actgttgccc ttgcggcaac ggggtatgaa tcacgttctg ttccgcaata cggactttgt
    15121 tgccatgcaa ttgggtaatg atggcgacat ggccagtgtc tttaaattca ccgcctttat
    15181 cccagataag aagcgccccc gctaccggtg cacgcggcga accgttagga aatgcctgca
    15241 atggcaggat gttgtcattg accacttcac gcaggaagcg cagtgagaaa atctcccatg
    15301 ccatacccac gtcagtgaag actacaccgt agttcagaaa gagaaaacgg cgagcaaatt
    15361 caacgcattg ccatttgtgg cccatatatt catcgtcgat atagctacgg aatacggcgt
    15421 catcttcgta ttcctgcgga tcgagagaac tgtaatctga agagtagatt gctaccccac
    15481 ctggggcgta gcccaataat gtcccgaacg gggcatcctg gctggtcgtt cctttgctca
    15541 tcactttacc tttaacaata caacctaagc agttggcgta attgttgtgc tctgattacc
    15601 tgctcagcga cattaaccgg acagaggtca gactaatcat acacctcatc gcagcggctg
    15661 gcgcaaaggg ttaaaaaatt cacattctgt acagcaagtg acctgctaca ctgcttcaac
    15721 actaccactc agaaggcaac tcactatgac agacaatact tatcagcccg cgaaagtctg
    15781 gacgtgggat aaatccgctg gcggcgcgtt cgccagtatc aatcgcccgg tttctggtcc
    15841 gacgcatgaa aaaacgctgc cggtgggcaa acatccattg caactttatt cgctgggaac
    15901 gccgaacggt cagaaagtaa cgattatgct tgaggagctg ctggcgctgg gcgttactgg
    15961 tgcagagtat gacgcctggc tgatacgtat tggcgatggc gatcaattct ccagcggctt
    16021 tgtcgaagtg aacccaaatt cgaagatccc ggcattgcgc gatcataccc ataatccgcc
    16081 gatccgcgtg tttgagtctg gatcgattct gctttatctg gcagagaaat ttggctactt
    16141 cttgccgcag gatctggcga aacgtactga agcaatgagc tggctgttct ggttacaggg
    16201 cgcggcaccg ttcctcggcg gtggttttgg tcacttttac cattacgcac cggtgaaaat
    16261 tgagtacgcc atcaaccgct ttactatgga agccaagcgc cttctcgacg tgctggataa
    16321 gcaactggcg cagcataagt ttgttgcggg cgatgagtac accattgcgg atatggcgat
    16381 ttggccatgg tttggcaatg tggtgttagg cggcgtatat gatgccgcag agtttcttga
    16441 tgcgggcagt tataagcatg tacaacgctg ggcgaaagaa gtgggcgaac gtctggcagt
    16501 gaagcgtggg cgtattgtta accgcatcaa cggaccgctg aacgagcagt tgcatgaacg
    16561 ccatgacgcc agtgatttcg agacgaatac ggaagataag cgtcaggggt aatggttagc
    16621 gttcgtcgca gcaagccatc cagaccaatg taggccggat aaggcgttca cgccgcatcc
    16681 ggcaatcgag cacaatgcct gatgcgacgc tgccgcgtct tatcaggcct acaaaagcta
    16741 atcatcacgc gctggtaatg tcgtaatcca tttggcgcag tgcgtctaat gtggctttgg
    16801 cttcgtcttc atcgataatg ctcatggcaa atccgacgtg taccagtacc cactggccca
    16861 gcagatcggc agggttacct tcacaaatca gggcgatatt cacgtcgcgc ttgataccac
    16921 atacttcaac ctgcgcaagc tggtgaatat cttcaccgac agccagcacc tggcctggaa
    16981 cgccaataca cattgttaac tccggttatt caacttcaat acttttgacg attagcgaat
    17041 cgccggtatc gacacgcaac cgctcgccat gacagatcgg acactgcgca tcgtgctgat
    17101 gaatctccac cacctggctg caatcccaac accaggcctg ggcgggttta tagacgatat
    17161 gtaaatcgca cccttgcgct accgttccgt ggcagacaat ttcaaaacta aaacggacgg
    17221 cgctctcctc aacgcaggag agtgcaccaa tttccagcca cacggcggtg acgcgcttaa
    17281 catcgtgctg ctccgcctgc cgttggataa tttcaacggc gctctggcaa agcgacaact
    17341 catgcatttt cgccactcct gcggccaaat agcagggcac gacgccctgc gtgtggtaca
    17401 tccggattgg tgactggcag cgaaaggatc attcgggcgc agtcatccgt cagccgttgg
    17461 ccctcttcaa tcgacatgct gtgcgaaagc ggcgacatca gcgagcagga gaggtattgc
    17521 gaaacaccgt ccagttcacc aacagtaaag gtcatagtgc catacggcag ttgcagacca
    17581 attttttcac tcactttgcg cagcggccag agttgatccg ggccggggaa gataactgca
    17641 ctcagcatcc acggggtgat cacacacccc gtccactgac cttcgaacag cgtaaagtca
    17701 gaaacataca ccggcattga aggatgcaga aaagagagat catgcatcga acgccgggca
    17761 atttcttcaa aagctacctg tacttgcgcc ttcggggagg tctggaaacc tgctatctct
    17821 tcagtcatga gtcgcctccc gtgggatagc ttccacgcct gattcgcgca gcgcagccag
    17881 aacctgctca agcgcaggtt caatcatcgc ttcgaccgtc ggtgtcaaac cgatgtgtgg
    17941 ctccagtgat tccgggatca cgccgaccag ggtcagcttt ttcggaaact cgccggtgaa
    18001 gcgcagggcc gacaatacgt cggccaggcc aagctgatgc ggagagattt tgttggtaaa
    18061 caacgccgga acttcttcat cccgcaggat catcatcgtt cccggtgcgt tctttttcga
    18121 cacaatggca tccgcaataa tcagatgatc gcgatttgcc atgtcgccaa gcagctccat
    18181 tcccgccgtg ccgccatcga ggatctcaac ataatccggc agaatgtatc gttgctctaa
    18241 cgcttcaaca atccgcacac cgatggcttc atcggtcagc aaaatattgc cgacccctaa
    18301 gactaaaata cgcattacag aaccttcact gaaaccactt cgttgccgtc ggcatccact
    18361 acgtgtaccg cacaggccat gcacgggtcg aaggagtgaa tggtacgcac cacttccagc
    18421 ggtttattcg gatcggcaac cggtgtaccc accagcgact gctcgtaagg accgacgtca
    18481 tcattgaagt tacgcgggcc agagttccag gttgatggaa caactgcctg gtagttgctg
    18541 atgataccgt ctttgatcac catccagtga gagagcatac cgcgcggcgc ttcaaggaag
    18601 ccaacgcctt tgaactcgcc cgttgccgga atgttcggct tcacaaaggt ggtgtgatcg
    18661 cctttgccga tattggtgat cagtgcactg tattgatttt gcaggatatc ctgcaattcg
    18721 cagcagtgaa cggtacgacc aataatacgg cccagcgtgg agtgcagttg cgctacttcc
    18781 agcgtgttgc cggtcagttt ctgataaatc gcaacgattt cattcagttt gttctgggta
    18841 gattcgcgac ctgccgccag tttcaccagc atattagcca gtggacccac ttctaccgtt
    18901 ttgccgtaga aagtcggtga tttcacccag gaatatttac cgtcgtcaga ccaaccgtcg
    18961 taagccggaa tggtggtacc ttcccacggt gcctgcggcg cttcgtcttt ataccaggag
    19021 tgcttcgcgc tttcctgaat tcctttgatc aggtattcat cggaatgaga agtgatcgga
    19081 cgatacgagg acagatccgc attctcaatg tagccacccg ggaacaggaa gctgccattt
    19141 ttactgtcgg tcgggaattc cggcacgctc aggtagttca ccgcaccttt accgcgcgtc
    19201 agccattccg ggtagaacgc agcgataact gcagtatcaa ccttgtaaac ctgctcaaca
    19261 aagtcgctca gcttgtcgat gaaggacttg atgtacatca ggcgctcaag gttcagcaca
    19321 cccagaccgt cgaggttgat cgggttcgcg acaccaccta ccgccaggtt ctgaatatgc
    19381 ggcgttttac cgcccagcag cgccacaacg cggttagcgt cacgctggca ctccagcgcc
    19441 tgcaggtagt gcgctaccgc aatcaggttc acttccggcg gcagtttcat tgccgggtga
    19501 ccccagtagc cattggcgaa aatacccaac tgaccgctgg caaccagatc tttgatcttg
    19561 ttctgaactt tggtgaactc ttccgggctg ttcagatgcc aggtcgaaac gcctttcagc
    19621 atttcggagg ctttggctgg atcagcttgc agtgcagaag tgatgtccac ccagtccagc
    19681 gccgaaagct gatagaaatg aacaatatgg tcatgcgtgg tgtgcgcagc cagaatgatg
    19741 ttacggatgt attgcgcgtt aaccggaacg tcgatattca gcgcactttc tgccgcacga
    19801 acggaagaca gcgcgtgagt ggtagtacat acgccacaga tacgttgcac aatcatccat
    19861 gcatcgcgcg gatcgcggtt tttcacgatc tcttccatgc cgcgccacat ggtaccggaa
    19921 gcccatgctt tcgaaacgac gccattttcg atttcgcaat cgatgcgtaa atgcccttca
    19981 atacgggtta ccggatcaat agtaattctc tggctcatgc tttgctcgcc tcatgacgat
    20041 tatgatcgtt ttgttttaaa ggaggaagta tcggcagtag acgaatgagt acgatataag
    20101 cgcaaatctc aatagccaca aaaccaatag aaatcaacag ttcttcccag gtcgggaagt
    20161 aggcgtaacc gccacccggg ttgaatgcca ccagcgaata ggtcagacgc caggttgcac
    20221 aacctaacag tgcactcagt gctgacagga acagcatgcg ggaatcatta cgcaacttcg
    20281 ccacacgcag aacgaccagc gggaagagca tcagcaggac ttcaatccag aacatcacgg
    20341 agtagaagtc accggcaaac gctaacgaca gcttgtcgcg atagataagc tcgccaaagc
    20401 gcagcacgat gaaaatcgcc agcaacacac tgatggtgtt ggtcagctta acgaacagac
    20461 tcttttcatc cggaccgttg ccacgcagac ccgcctgcac caacgaacct tcaaagatga
    20521 caatcgagaa gcccatgatg aacgccgtca gcagcgagaa cagcggcaac atttcatagc
    20581 tttgccacag cggatgcacc ttgtagcccg ccgagatcat cagcgacccc attgaagact
    20641 ggtgcatagt tggcagcagc gcaccaagcg cgatgatgaa gaacatcacc ttgttcagac
    20701 gtttgagtga caccttccag cccagacgtt caaacaatgc cggagcaaac tccagtgcca
    20761 tcacaccgat gtagatggtc atacagaccg ccgtctcgaa cagtaccgag ttcacgttga
    20821 agtgacccgg aatgtagaag tacggcaggt tccagtagcg acccacgtcg atagtgatcg
    20881 acaagccacc cagtgaataa ccaaacagac tcgccaacag cgccggacgc accagcggat
    20941 ggtattgccc acggttaaag acgtatactg cccacgccag cgcccagccg ccacaggcaa
    21001 agccggtacc gatcagcagg tcaaacgcga tccacacgcc ccacgggaag ccgccgttca
    21061 ggtcggagac agagcccaga ccgaacacca gacgcttcac aatcaggagc atacagatga
    21121 cgattaacgg tccaaaaatc atgaccggtt tactgatgat tttgccgccc agcggttgtg
    21181 gatcatgact catgatcgtc tcctccgtcg tgatggtcgt ttttagtgtt gcgacgaacc
    21241 agcacggtta agcccgccag cacagccagt ggtagcatca tgcctttata cagggtgtgt
    21301 tggacatgtt cggaacgcgc accagtagaa agatcgtcca gtttcggcag gtcgagattt
    21361 tcataaggca cacccgtcag taccagaacc tgagtaccgc cgccctcttt ctcgccgtac
    21421 agatgcggat aatatttcgg caccgtatgc aggtaagtgt cgccagattt cagcgtctga
    21481 cgcggatagt ggtattcgct gcccggcttc agcgccagac gttttttcgc ctccgccatc
    21541 agctcttcac gcgtaccaaa aatcaccgcg cccgccgggc acacttctac acagccaggc
    21601 agaccgcctt tatcgagacg ttccacacct ttctggttgc acaactcgca cttatgcagc
    21661 gcaccaaacg ggttgttgta gtcgtacttc ggcacgttgt atgggcaggc gaccatgcag
    21721 taacggcaac cggtgcacac atctttgtcg taatggacaa taccggtttt cggatctttt
    21781 ttcagtgcag agaccggaca cacagagaca cagttcggat cgacgcagtg catacactgt
    21841 ttcttaatgt acgcgtagcc attctcctcc tggtctttgt tgacccctgt gccgctggtc
    21901 cacacctgaa tgatgttatt ggtatacggc gacagtttgt cgttgttcga ccaggtctgt
    21961 tccccttgcg ggttacgttc agggaagttg atatcctgac acttggtgac gcaagcctga
    22021 cagcctacgc acaaggtcga gtcgtacaac atccccagcg atcccggaat tggcgggcgg
    22081 ttttcagcag ccgcatgact gacagacggc agcgcgcccg tcagcaatgc cccgcaggag
    22141 gctgctttaa taaaattacg tctgttcacg gttattctcc ccgtgagtca gcgttatctt
    22201 tcttttgctg acgacccagt tcacgcaccg ccatcacgct gacaccggca accagcccaa
    22261 ccacaccgcc gagcaaacca atagcgcctg ccgagacgtt gccgccttct ttagcgttaa
    22321 cgtccggttt ctgtgaacgc ggagtctggt tttcgacgtt ggcaagctga tggatgcctt
    22381 tatggaagcc gataccttct tcgttacagc catagcacgg gtgaccaatc gctaccggcc
    22441 acacgccgcc aacatcgcag aattgcagcg ttgagcagtt gccgtaagtt tctggccctt
    22501 tacagccgag gtggtacagg caccagcctt cgcggtggcc ttcatcaccg aactctttgg
    22561 caaaacgacc agcatcgaag tgcgggcgac gttcacagtg ttcgtgaatc agacgaccat
    22621 aggcgaaagt cggacggttt ttgtcatcca gtttcggcgg tttgccgtaa gtgatgatgt
    22681 gcgcaacggt cgccaggaag ttgtgcgggt tcggcgggca gccaggaata ttgataacgg
    22741 ttttgcccgg tagaacttct tgcaggctga ctgcgccagt tgggttaact ccagctgcgg
    22801 caacaccgcc ccacgcagag caggaaccga tagcgataat ggctgctgcg ccttccgccg
    22861 ctttgcggat gtgatccaca atcggctcac cggcaaccat gcaataaata ccgttatctt
    22921 ttaatgggat ggaaccatcc accactaaca catactgccc tttgtacttc tcgagagcgt
    22981 tatgtttgtt ctcttcgacc tgatgaccga aggcggcgga aagcacttcg tgatactcca
    23041 gagagatagt ctccagcacg aggttttcta ccgttggatg cgttgcacga agcagagatt
    23101 ccgtacaacc ggtgcactcc tgcgcgccaa tccagataac tggcggacgc tgcgggttag
    23161 taaccgattc gaccatctct gcagcggctt tgctacttaa ccccatggtg gcggctaatg
    23221 ctgcacaaag cttcatgaaa tcacgacggt taatgccgtg agaatggatg agggtgttat
    23281 ctccagtcat ttatagttat tccgttgcga agacctggca tatattttgc ctcaatcgca
    23341 aaatcaataa tgcgatcgat gcgccattta ccacacattt attatggtta tcgatatcat
    23401 gatactgcgg cggggcacta aaacgaaggg aagaagcatt agtgtaatta attaaagcaa
    23461 gataatacgt atgtttgatc aattttctag gcgataaccg gccataaaaa agcgcaacgt
    23521 agaacaggaa ttattctcac gaaagaaatc cattaccatc tctttatcca gtccataacg
    23581 gcgcgtcaga atacccgctt cccaggcgct aagctggcga tctccggtct tttcgtacat
    23641 ccggtgtgag tagcctaata caaaaccgcg tttataatcg gcacagaatt ttgtcacatt
    23701 aaccgcactg tcggcatgcg ttgcttttaa tcccgccatt aagcctttac caaaatggtt
    23761 attcatcgtt cacctcattc gctatatatt gtcatatata gcgatatttt aggcaaagaa
    23821 cgacatttgc gcacgctatt ttttcatgct tgtttaagac gtaatcctgt aagagataaa
    23881 cccatcagga gagatattta tcgaaccatt tcagcgtcct ttgccaggca agatcggcgg
    23941 cagatttgtc ataacggggc gtggaatcat tatggaatcc gtgattaacc cccggatata
    24001 tatacgcctc atagacctta ttattggctt tcaacgccgc ctcgtaagcg ggccagccct
    24061 cgttgattcg gctatccagt tcagcgtagt ggagcagtaa aggcgcttca atcttcgcca
    24121 catcggcagt gggtgcctga cgcccataaa acggcaccgc gcaggccagt tccggatacg
    24181 cgactgctgc cgcgttcgat acgcccccgc catagcaaaa tccgctaata cccactttgc
    24241 ctgtcgcttg cggatagcgt tgcataaact caattgcggc aaagaaatca ttcatcagtt
    24301 tggttggatc aacctgctgt tgcagttcgc ggcctttatc atcgttacca ggatagccac
    24361 ctactgagct taaaccgtct ggcgccaggg cgatgtaccc ttccttcgcc actcgccgcg
    24421 tcacatcttc gatatacgga ttcagccctc gattctcatg caccactacc acggctggcg
    24481 ttttaccgct catttttgcg ggcttcacca gataaccccg tacctcgccg tgaccatttg
    24541 gcgagggata cgtgatgtac tctgcaacaa tttccgggtc ggtaaactct acctgagtcg
    24601 ccagcgcata atttggcttg agcaaatcaa acagcgctaa tgccgtcatc ccgcccaccg
    24661 catacttcgc cgcaaggttg aggaactcac gtttcgagat tttcccgtga gcgtaatagt
    24721 cgtagtaatc cagcaactct tgtgggaaag ctttggcagt taaacgcggc atcgttgcac
    24781 tcctcaactg gtgttttttt aagcaaagca taagcgcgtg tttttgccca gtttttcgtc
    24841 actctgtgag ccagactacg ggatacgcgc tggcgaatcg ctaaactaga aacattgttt
    24901 cgaaattgaa cggtggaaag gagaggtcat ggtctggtta gcgaatcccg aacgttacgg
    24961 gcagatgcag taccgctatt gcggaaaaag tggtttacgc ctacccgcgt tatcgctcgg
    25021 tttatggcac aatttcggtc acgttaacgc gctggaatca cagcgtgcga tcctgcgtaa
    25081 agcgtttgat ttaggcatta cgcactttga tttagccaac aattacgggc cgcctccagg
    25141 aagcgcagaa gagaactttg gtcgcctgct gcgcgaggat tttgccgctt atcgcgatga
    25201 actgattatc tctaccaaag ccggatacga tatgtggccc ggcccatacg gctctggcgg
    25261 ctcacgtaaa tacctgctcg ccagcctcga ccaaagcctg aagcgtatgg ggcttgagta
    25321 tgtcgatatc ttttactctc atcgcgtcga tgaaaatacg ccgatggaag aaaccgcctc
    25381 tgcgctggct catgcggtac aaagcggtaa ggcgctgtat gtcgggatct cctcttactc
    25441 gccagagcgg acgcaaaaaa tggtcgagtt gctgcgcgag tggaaaattc cgctgttaat
    25501 tcatcaacct tcgtacaatt tactgaaccg ctgggtggat aaaagcggcc tgctggatac
    25561 cctgcaaaat aacggcgtgg gctgcattgc ctttactcca ctggctcagg gattgctgac
    25621 cggaaaatat ctcaacggta ttccgcaaga ttcacggatg catcgtgaag ggaataaagt
    25681 tcgtggtctg acgccgaaaa tgctcaccga agccaacctc aacagcctgc gcttattgca
    25741 tgaaatggca cagcagcgtg gacaatcaat ggcgcaaatg gcgttaagct ggttgctgaa
    25801 agatgagcga gtgacgtcgg tattgattgg tgccagccgc gcggagcaac ttgaggagaa
    25861 cgtgcaggcg ctgaataatc tgacatttag caccgaggag ctggcgcaga ttgatcagca
    25921 tatcgccgat ggagagctga atctgtggca ggcgtcttcc gataaatgac ctgttaataa
    25981 cgggcgcgag aaccgcgccc gaatcagatc agtgattatg acgagtcagt cggtcaagat
    26041 agcccatcac aaatgcagag agcacaaacg tcagatggat aatgacgtac cacatcagtt
    26101 tgttatcagg gacatttttc gcatccataa agacgcgcag taagtgaatg gaagaaattg
    26161 ccacaatcga cgctgctact ttgtttttca gcgacgttgc gtccattttc cccagccagt
    26221 tcagcttctc tttgttctca gagatatcga gttgtgagac gaaattctcg taaccggaaa
    26281 acatcaccat caccagcaaa ccgccaacca gcgtcatatc caccagcgac agcaacacga
    26341 ggatcaaatc tgattccgcc atcgagaaga tattcggcag tacgtgaata atcacctgga
    26401 agaacttcag cgccagggca actaacgcca gcgaaaggcc aaagtacacg ggggcaagca
    26461 gccagcgaga agcatacatt gcattttcaa gaaaacgttc catacattcc tgtcgtgtag
    26521 ttaaaacggg caacagtata tcgcaattaa gtaaacagca cgcaacagca aatcaacata
    26581 tacgttaaac ctttgtcgca taattctgag ggttagggat cttcctggtt cccttatcaa
    26641 cgcatcgaga gcgtctaccc ttaaaggacg ttgaacctga agggagaaaa acgatgtctc
    26701 atttaaaaga cccgaccacg cagtattaca ctggtgaata tcccaaacag aaacaaccga
    26761 cgccaggcat ccaggcgaag atgacaccgg taccggattg cggcgagaaa acctatgttg
    26821 gtagcggtcg cctgaaagat cgtaaagcac tggtgacagg gggcgattcc ggaatcggtc
    26881 gcgctgccgc cattgcttac gcgcgtgaag gggctgacgt ggcgatcagt tatcttcccg
    26941 tggaagaaga agacgctcag gatgtgaaaa agatcattga agaatgcgga cgcaaagccg
    27001 ttctgctgcc aggcgattta agcgatgaga aattcgcccg ttcgctggtt cacgaagcac
    27061 acaaggcgtt aggcgggctg gatattatgg cgctggtagc cgggaaacag gttgccattc
    27121 ctgatattgc agacctcacc agcgaacagt ttcaaaagac ctttgccatt aacgttttcg
    27181 cgttgttctg gctaacccag gaagcgatcc ccctgctacc gaaaggtgca agtatcatca
    27241 ccacttcctc aatccaggca taccagccaa gcccgcattt actggactat gcggctacga
    27301 aggcggcaat tctgaactac agccgtggcc tggcaaaaca ggtcgcggag aaaggtattc
    27361 gggtgaatat tgtcgcgcca ggcccgatct ggacagcact gcaaatttct ggcggacaaa
    27421 cgcaggataa gatcccgcag tttggtcaga aaacgccgat gaagcgtgcg gggcaaccgg
    27481 cggagctggc ccctgtatat gtttacctgg caagtcagga gtcgagctac gtcaccgcag
    27541 aagtgcacgg tgtctgcggc ggcgagcatt taggttaaaa aaatgcccgg ttgtgaaaag
    27601 caaccgggca tcatttttat ttcgctttcg cagtttcttc gccgaccaga cctattttca
    27661 ggtaacccgc ctgatgcagc gtatccatta ccttcatcaa cgtctcgtaa tcgacagttt
    27721 tatccgcacg gaagaagatg gtggtgtctt tcttgccttc ggttaacgca ttcaacgccg
    27781 taatcattgt ttcatcggtg accggatcgt taccaataaa catcgagttg tctgctttca
    27841 ccgacagata aaccggtttt tccgggcgcg gctgcggcgt gctggtagaa gcaggcaaat
    27901 tcaccttcac atctaccgtc gctaacggtg ccgccaccat aaagataatc agcaaaacca
    27961 acatcacgtc gataaacggt gtcacgttga tatcatgcat ttcgccgtta tcgtcgaggt
    28021 tttcgttaag atgcatcgcc atcggacatt atcctgcacg taatttttgt gcgacacgaa
    28081 ccggatgcgc agcggcgctg gcttccagat ccaggtcacg gctttgcagc aacaatacct
    28141 gcgctgcaac atcacccagc atcgctttaa agccgccaat ctggcgtgca aatacgttat
    28201 agataacgac cgcaggaatc gctgccacga ggccgattgc cgttgctaac agagcttctg
    28261 caatacccgg cgcaacgact gccaggttag tggtctgcgt ttgcgcgata ccaataaagc
    28321 tgttcatgat gccccagacc gtaccaaaca gaccaacgaa cggcgaaatc gcgccgatgg
    28381 ttgccagata gccgttacca cggcccattt gacgacctac tgcggcgacc cgacgctcca
    28441 gacggaagct ggtacgttct ttaatacctt cgttatcgtc gctgccttct gacagttcca
    28501 gctcattctg cgcttcattg agcaaatgca ggctcaagct tttgctacca aaatcagcgg
    28561 cgatatcgtt ggcctggttt aaggaacgcg cttcagccag cagttgctgc tcgcgcttaa
    28621 gacgacgctt ctgattgaag aactccacgc tcttactgaa gaagattgcc caggtgacta
    28681 cggaggccaa aataagccca atcatcacgc acttaacgac aatatcggcg tgctgataca
    28741 taccccagac ggagaggtcc gtctgcatta aattattacc cactgtgtat ctccaggacg
    28801 caagtcacaa aatctgtgca taataatatc aaaacgacgt cgaattgata gtcgttctca
    28861 ttactatttg catactgccg tacctttgct ttcttttcct tgcgtttacg cagtaaaaaa
    28921 gtaaccagca cgccatttgc gaaaattttc tgctttatgc caattcttca ggatgcgccc
    28981 gcgaatattc atgctagttt agacatccag acgtataaaa acaggaatcc cgacatggcg
    29041 gacaaaaagc ttgatactca actggtgaat gcaggacgca gcaaaaaata cacgctcggc
    29101 gcggtaaata gcgtgattca gcgcgcgtct tcgctggtct ttgactgtgt ggaagccaaa
    29161 aaacacgcga cacgtaatcg cgctaatggc gagttgttct atggacggcg tggaacgtta
    29221 acccatttct ccttacaaca agcgatgtgt gaactggaag gtggcgcagg ctgtgtgcta
    29281 tttccctgcg gggcggcagc ggttgctaat tccattcttg cttttgtcga acagggcgat
    29341 catgtgttga tgaccaacac cgcctatgaa ccgagtcagg atttctgtag caaaatcctc
    29401 agcaaactgg gcgtaacgac atcgtggttt gatccgctga ttggtgccga tatcgttaag
    29461 catctgcagc caaacactaa aatcgtgttt ctggaatcgc caggttccat caccatggaa
    29521 gtccacgatg ttccggcgat tgttgccgcc gtacgcagtg tggtcccgga tgccatcatt
    29581 atgatcgaca acacctgggc agccggtgtg ctgtttaagg cgctgggttt tggcattgat
    29641 gtttccattc aggccgctac caaatatctg gtggggcatt cagatgcgat gattggcact
    29701 gccgtgtgca atgcccgttg ctgggagcag ctgcgggaga atgcctatct gatgggccag
    29761 atggtcgatg ccgataccgc ctatataacc agccgtggcc tgcgcacatt aggtgtgcgt
    29821 ttgcgccaac atcatgaaag cagtctgaaa gtggctgaat ggctggcaga acatccccaa
    29881 gtagcgcgag ttaaccaccc tgctctgcct ggcagtaaag gccacgaatt ctggaaacga
    29941 gactttacag gcagcagcgg gctattttcc tttgtgctta agaaaaaact caatgatgaa
    30001 gagctggcga actatctgga taacttcagt ttattcagca tggcctactc gtggggcggg
    30061 tatgaatcgt tgatcctggc aaatcaacca gaacatatcg ccgccattcg cccacaaggc
    30121 gagatcgatt ttagcgggac cttgattcgc ctgcatattg gtctggaaga tgtcgacgat
    30181 ctgattgccg atctggacgc cggttttgcg cgaattgtat aacattgcca cttttggaca
    30241 attttgcaga cattttattg tgaaaagtct taaattgttg cgtccgggat caaggcgtcc
    30301 cggacgattc aggagtacaa taggcagata aaggcttaaa cgctgttcca caggaaagtc
    30361 catggctgtt attcaagata tcatcgctgc gctctggcaa cacgactttg ctgcgctggc
    30421 ggatcctcat attgttagcg ttgtttactt tgtcatgttt gccacgctgt ttctagaaaa
    30481 cggcttgctg cccgcctcat ttttgccagg cgacagcttg ttgatattgg ctggcgcatt
    30541 gattgcccag ggggttatgg attttctgcc tacgattgcg attctgaccg ccgcagcaag
    30601 tctgggctgc tggctaagtt atattcaggg gcgctggtta gggaatacca aaacggtgaa
    30661 aggctggctg gcacagcttc ctgctaaata tcaccagcgc gccacctgca tgtttgaccg
    30721 ccacggtctg ctggcgctgc tggctggacg ttttcttgca tttgtccgta cgctgctgcc
    30781 aaccatggcg ggaatttccg gtctgccaaa ccgccgcttc cagtttttca actggttaag
    30841 cggattgctg tgggtcagcg tggtaaccag tttcggctat tccttaagta tgattccgtt
    30901 cgttaaacga catgaagatc aggtaatgac gttcctgatg atcctgccaa ttgccttgtt
    30961 aaccgctggc ttgttgggca cgctgtttgt ggtgattaaa aaaaaatact gtaacgcctg
    31021 acgattttcc ccgttcccgg ttgctgtacc gggaacgtat ttaattcccc tgcatcgccc
    31081 gcattcttgc cgcatcttcc cccggcgtca caccgaagta acgtttaaac tcacggctaa
    31141 attgcgatgc gctttcatag ccgacgcgca tcgccgccgc gctggccttc atgccgtcgt
    31201 gaatgatcat catccgcgcc ttatgcagac ggtaattctt caaatactgc aacggcgagg
    31261 tgctggtgac agacttaaaa ttatggtgga acgccgatac gctcatgttg gcttctgccg
    31321 ccagttgctc gacgctcagg ttttcggtgt atttattctc aatccgtttc agcacgcgac
    31381 taatcaggct gaagtgagtc tggcggctga ccagtgccag taacgcgccg ccgcaaggtc
    31441 cggtcagcac gtagtacaga atttcgcgga taatctgttt gccgagaatg cgcgcatcca
    31501 gcggtcgctc catcacatcc agtaaccgct ctgccgcgca taaaatctct tctgataacg
    31561 tggcggagtt aataccgctg gctgccattg acggctgaaa atgttcatct tcgccaatgt
    31621 ccatcaacag ttcctgtaac tgcaaaatat cgacattgag acgcaaccct gccaacggca
    31681 cctctgacgt ggcataggtt tcgcactcaa acggcaacgg caccgtcagc aggagatatt
    31741 cattagcgtc ataacgaaac acgcgttcat tgatataacc gattttatgt ccggaaaaga
    31801 gaattatgat gccaggctcg tacatcaccg gtgtgcgtgc gaaaggcgtc tcgccataca
    31861 acaaacgcac atcgggcaac agctctgaca aactattttc tttatttttc agtttattaa
    31921 ctttatccgc cagcaagcgg caaatctctt cacgtttcat atcgcgtaat ttcttaggaa
    31981 taatgcggca atttgattgt gcgcaatttt gtagcatttc tccagcactc tggagaaata
    32041 ggcaagacat tggcagaaat gagcattgag aggtacggcg ctggcgacca caatgaaaaa
    32101 catcaggcag accgatctct gccctcatat tgacccagca aagggagcaa gtaatgaaca
    32161 actttaatct gcacacccca acccgcattc tgtttggtaa aggcgcaatc gctggtttac
    32221 gcgaacaaat tcctcacgat gctcgcgtat tgattaccta cggcggcggc agcgtgaaaa
    32281 aaaccggcgt tctcgatcaa gttctggatg ccctgaaagg catggacgtg ctggaatttg
    32341 gcggtattga accaaacccg gcttatgaaa cgctgatgaa cgccgtgaaa ctggttcgcg
    32401 aacaaaaagt gacttttctg ctggcggttg gcggcggttc tgtactggac ggcaccaaat
    32461 ttatcgccgc agcggctaac tatccggaaa atatcgatcc gtggcacatt ctgcaaacgg
    32521 gcggcaaaga gattaaaagc gccatcccga tgggctgtgt gctgacgctg ccagcaaccg
    32581 gttcagaatc caacgcaggc gcggtgatct cccgtaaaac cacaggcgac aagcaggcgt
    32641 tccattctgc ccatgttcag cccgtatttg ccgtgctcga tccggtttat acctacaccc
    32701 tgccgtcgcg tcaggtggct aacggcgtag tggacgcctt tgtacacacc gtggaacagt
    32761 atgttaccaa accggttgat gccaaaattc aggaccgttt cgcagaaggc attttgctga
    32821 cgctgatcga agatggtccg aaagccctga aagagccaga aaactacgat gtgcgcgcca
    32881 acgtcatgtg ggcggcgact caggcgctga acggtttgat cggcgctggc gtaccgcagg
    32941 actgggcaac gcatatgctg ggccacgaac tgactgcgat gcacggtctg gatcacgcgc
    33001 aaacactggc tatcgtcctg cctgcactgt ggaatgaaaa acgcgatacc aagcgcgcta
    33061 agctgctgca atatgctgaa cgcgtctgga acatcactga aggttcagac gatgagcgta
    33121 ttgacgccgc gattgccgca acccgcaatt tctttgagca attaggcgtg ccgacccacc
    33181 tctccgacta cggtctggac ggcagctcca tcccggcttt gctgaaaaaa ctggaagagc
    33241 acggcatgac ccaactgggc gaaaatcatg acattacgct ggatgtcagc cgccgtatat
    33301 acgaagccgc ccgctaagct ttttacgcct caaactttcg ttttcgggca tttcgtccag
    33361 acttaagttc acaacacctc accggagcct gctccggtga gttcatataa aggaggaacg
    33421 tatggctaat ccaaccgtta ttaagctaca ggatggcaat gtcatgcccc agctgggact
    33481 gggcgtctgg caagcaagta atgaggaagt aatcaccgcc attcaaaaag cgttagaagt
    33541 gggttatcgc tcgattgata ccgccgcagc ctacaagaac gaagaaggtg tcggcaaagc
    33601 cctgaaaaat gcctcagtca acagagaaga actgttcatc accactaagc tgtggaacga
    33661 cgaccacaag cgcccccgcg aagccctgct cgacagcctg aaaaaactcc agcttgatta
    33721 tatcgacctc tatttaatgc actggcccgt tcccgctatc gaccattatg tcgaagcatg
    33781 gaaaggcatg atcgaattgc aaaaagaggg attaatcaaa agcatcggcg tgtgcaactt
    33841 ccagatccat cacctgcaac gcctgattga tgaaactggc gtgacgcctg tgataaacca
    33901 gatcgaactt cacccgctga tgcaacaacg ccagctacat gcctggaacg cgacacacaa
    33961 aatccagacc gaatcctgga gcccattagc gcaaggaggg aaaggcgttt tcgatcagaa
    34021 agtcattcgc gatctggcag ataaatacgg caaaacccct gcgcagattg ttatccgctg
    34081 gcatctggat agcggcctgg tggtgatccc aaaatcggtc acaccttcac gcattgtcga
    34141 aaactttgat gtctgggatt tccgtctcga caaagacgaa cttggcgaaa ttgcaaaact
    34201 cgatcagggc aagcgtctcg gtcccgatcc tgaccagttc ggcggctaac gtgcaaattc
    34261 tcccggtggc ggtaatgttc cgctaccgga cttttcagaa atcatttatt cccctcgcgt
    34321 cccgcccgtt gctactcttc cttgttcagg aatgccaaat ataaggacat catcatgcag
    34381 agccggaagc tcttaaaaga acaactcatc tatatccggg ataaacgcaa cggagaggtg
    34441 aaaaacagat gaaaataata cttctgtttt tagcagccct ggcaagtttt accgtacacg
    34501 cacagccccc ctcacagacc gtagaacaaa cagtccggca tatttatcag aactataaat
    34561 cagatgccac tgccccttat tttggtgaaa ccggagagcg agcgataact tctgcgcgta
    34621 ttcaacaggc gcttaccctg aacgacaatc ttacgctgcc gggcaatatt ggctggctgg
    34681 attatgatcc ggtttgtgat tgtcaggatt ttggcgatct ggtgttagaa agcgtagcga
    34741 taacccaaac tgacgccgat catgccgatg ccgttgtgcg ctttcgtatc tttaaagacg
    34801 ataaagaaaa gacctcgcag acactgaaaa tggtggcgga aaatggtcgt tgggtcattg
    34861 gcgatattgt cagcaatcat ggcagcgtct tacaagcggt taatagcgag aatgaaaaaa
    34921 cgctggccgc tatagcttcg ttgcaaaaag aacagccgga agcctttgtt gccgaactct
    34981 ttgaacatat tgctgattat agctggccgt ggacgtgggt ggtttccgac tcttaccgcc
    35041 aggcggtcaa tgccttctat aaaaccacct tcaagacggc caacaatccc gatgaagata
    35101 tgcaaataga acggcaattt atttactaca atccgatctg ttttggcgaa gagtcgttat
    35161 tttcacgcgt tgatgaaatt cgagtcctgg agaaaaccac cgattccgcc cgcattcatg
    35221 ttcgctttac gctgaccaat ggcaacaacg aagagcaaga actgatttta cagcggcgcg
    35281 aaggtaagtg ggaaatcgct gattttatcc gcccgaacag cggcagccta cttaagcaga
    35341 ttgaggcaaa aaccgccgcc agattaaaac aatgagctga attaaataac aattagccgg
    35401 aacaataaat aaaagggaac actatatgaa aacgattttc accgtgggag ctgttgttct
    35461 ggcaacctgc ttgctcagtg gctgcgtcaa tgagcaaaag atcaatcagc tggcgagcaa
    35521 tgtgcaaaca ttaaatgcca aaatcgcccg gcttgagcag gatatgaaag cattacgccc
    35581 acaaatctat gctgccaaat ccgaagctaa cagagccaat acgcgtcttg atgcacagga
    35641 ctatttcgac tgcctgcgct gcttgcgtat gtacgcagaa tgataaaaaa atccccggca
    35701 gcattctgtt accggggata ttttttaacg tccaaccgcc gctttagggc gtttcttcgc
    35761 accagcattc tgcaggcgag attgcgtaga cgacgctttt tttgccgtag caggcgtctg
    35821 acgctgggtc gccatcggcg tatgtttagt caacgccgga cgggtattgc ggttctgacg
    35881 gcgagcttca cgcatctctt caatggttgg cgcaggcact aagcaatcgc gacggctgcc
    35941 aatcagatgc tttttgccca tcgcttccag cgcctggcgg attaacggcc agtttgccgg
    36001 atcgtggtaa cgcaacaacg ctttatgcag gcgacgctgc ttgttgccct tcggtacgaa
    36061 gacgtcttca ctcttataac caatcttcgc cagcgggttt ttgccggtgt aatacatggt
    36121 ggtcgagtta gccagcggcg acggatagaa gttctgcacc tggtcgaggc ggaagcgatg
    36181 ctttttcagc catagcgcca gattcaccat atcttcatca cgcgtacccg ggtgcgcgga
    36241 gatgaaatac gggatcagat actgttcttt acctgcctgt ttcgagtaag tatcgaacag
    36301 ctctttaaag cggtcatagc tgcccatgcc cggcttcatc atcttcgata acggcccttc
    36361 ttcggtatgt tccggggcaa tcttcagata accgccaacg tgatgggtcg ccagctcttt
    36421 gatatagcgc ggatcttcta cggcgatgtc ataacgcaca ccagaggcaa tcaggatctt
    36481 tttaatgcct ttcagatcgc gcgcacggcg atagaggtta atcgtcggtt cgtggttagt
    36541 gtccatatgc ggacaaatat ccggataaac gcacgacaaa cgacggcagg tttgctcagc
    36601 gcgtggcgat ttgcaacgca acatatacat gttggcagta ggcccaccga gatcggaaat
    36661 cacgcccgta aaacctggaa cagtgtcgcg gatcgcttcg atctcattaa taatcgaatc
    36721 ttcggaacgg ctctgaataa tgcgcccttc gtgctcggta atagaacaga aagagcagcc
    36781 gccaaagcag ccacgcataa tgttgaccga gaaacgaatc atttcgtaag ccggaattcg
    36841 ggcattaccg taagccggat gcggcacgcg cttgtacggc agcgcaaaga cgctatccat
    36901 ctcttcggta gaaagcggaa tagcaggcgg gttgatccat acatagcggt cgccatgttt
    36961 ttgcatcaat gcgcgggcgc agcctgggtt agtttcgtgg tgcagaatgc gcgaagcatg
    37021 ggcgtacagc actttatcgc ccttcacttt ctcgaaagaa ggcagcaaca cgtaggtttt
    37081 ttcccacggt ttcgggcgtg gtggctgcac ggttacagct ttggcttcct gctttttcgg
    37141 agctaccggt ttgttatccg cgcacggcaa atcttcgcca tacggatgcg ggattgggtc
    37201 gattttccct ggggtatcaa gacgggtgga atccacaccg ctccagccag gcaacgcctc
    37261 ttttacgata atcgcggtat tacgcacatc gcggatttca ctaatcggct cgcccatcgc
    37321 cagacggtgc gccacttcca ccagcggacg ctcgccgtta ccgaacatca gcatgtcagc
    37381 tttggaatcc accaacaccg aacggcgcac ggtatccgac cagtaatcat aatgcgcggt
    37441 acggcgcagg ctggcctcaa taccgccgag gatcaccggc acatctttcc acgcctcttt
    37501 acaacgctgg gtataaacca gtgtggcgcg atccggacgc ttacccgcga cgttatccgg
    37561 tgtgtaggca tcgtcatgac gtaaacggcg atcggcggta tagcggttga tcatcgagtc
    37621 catgttgcca gcagtaacac cgaaaaacag attcggttta cccagacgca taaagtcgtc
    37681 tttactgctc cagtctggct gggcgatgat cccgacgcga aagccctgcg cttccagcat
    37741 acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc cagtaaccaa
    37801 aatgatgtcg cagctatcct agccaagttg atccatctct tcacgtgaca tcggcaaaaa
    37861 cggtgccggt ccaaaacagg cggcccagta ctgcggccag gagaacaggt cgcgatccgg
    37921 ttggatcagg gagatagagc tcattttgct tccagaaatg gtaaaaaaat aatcaaaggc
    37981 cggggattat acgctgtatc acgcgcggaa atgaagtgat gttgatgaac ggtggcgaaa
    38041 taccctggtg aggtttttca ctgcccgact tgggcaagca catttaaggc tggcggtatc
    38101 gtggcaactg ccgctactat ttatgccgat cagcaacgtt caaatcatat tgcacgacga
    38161 tcggctatcc gaggacaccc acagtaaggc aaggtatgga acaggttatt acaaagcgcc
    38221 gctattacga tattggtcta cagattgaag agctgctgta ttcaggcgta ttcaaagccg
    38281 gagaacgctt accttcagaa cgtgaactga gcgagcggtt taataccagc cgaactacca
    38341 ttcgtgaagc cattattatg ctggaactga aaggcgtatt gaatgtgaag caagggtcag
    38401 ggattttctt tgtcgactcc accgataagc tcaaccagaa atcattaatg ccctattcag
    38461 aaattggccc gtttgaactg ttgcaggcgc gtcaggtgat tgagagcaat attactggtt
    38521 ttgcagccag ccagattagc tttaacgagt tacaagagtt aaagaaaatc attggattac
    38581 aggaaaaagc catcgctgcg gaaagtgata aatttgaaga cctggatcac cgatttcata
    38641 gcattattgc cgaagcgacg caaaatcggg tactcatcaa acaagccgct gaattatggc
    38701 gggcagtgcg cacagaaaac ccccgctgga agaaacttaa ctacaagtac ctgcatgaaa
    38761 agcatttgcg tctgcaatgg ctggaagatc accgggctat tttcctggct ctgcaacaga
    38821 aagattcaga gctcgccagg gaagcgtcct ggagacatct ggaaaacagc aaaaatgaac
    38881 tgatcaaaat cttcaaacaa gacgcttcca tcagcgattt tgatgacttt ttcttcgctc
    38941 gttaaccttc attagtccag agctgacgtg aaaaatgacg ctggctctgc gttgcaccct
    39001 ccactggtat acgctccctt ctcaattggt ctaataaatc gattatcatc agatatctta
    39061 gattagcggc aattatccca aaaactgacc tctaaaaaac tttcctttca ttttattcat
    39121 ataactcatt gatacagata taaataacgc cttgttgttc tgtttcgtac ttttgccttt
    39181 ctctgcaagc gtgatgcatc tctctgtttt tggtctagtg aattatgtaa attggtctat
    39241 acacaccagc taccaggagt aagagcataa tgtcagggaa cgaaagtatc gatgccgccc
    39301 actatgatca ggtaaccacc tggcctcggg acgagttaca ggcagatatt gtccatattg
    39361 gttttggcgc ttttcatcgc ggacaccagg ctgtctacac ggatctcact aatcagcttt
    39421 cggacaccca ctgggggatc tttgagatca acctgtttgg tgatgctcaa ctgatcgaaa
    39481 acttaaatgc gcaaaatggg ctgttttcgg ttgtggaaac atctgcatcg caatctacct
    39541 cacgccttgt gcgttcggta actggcggta ttcatacccc cgtggatggc attgccgcag
    39601 caatccagaa attgactgaa cctcaggtaa aaatcgtttc attaaccatc accgagaaag
    39661 gctattgtct cgattcgcaa acgcgatcgc ttgatctcac caatggatta atcaaacacg
    39721 atctacaaaa cccggatgcg cctcagtcgg ctattggctt gatcgtctgt gctttgcaac
    39781 aacgtaaagc ggcagggctc gccgctttta gcgtgctctc ctgtgacaac ttgccagaca
    39841 atgggcatct gacgcgcaat gccgttctcg gttttgcccg acaactggac cagcccctgg
    39901 ctcagtggat cgaagaaaat gtctcatttc caggcacgat ggtcgatcgc attgttccgg
    39961 caatgactga atcgcaattc gccttactgg aaactaaaac cggttatgcc gatccctgcg
    40021 ggatcgtctg cgaatcattt cgtcagtggg tgatcgaaga taattttgtg cgcggacgac
    40081 cggaatggga taaagccagc gcgatgtttg tcagcaatgt tcagccttat gaagagatga
    40141 agttacgcat gttaaatggt agccattcat ttctggctta taacggctca ctggctggct
    40201 atgagtttat ctggcaatgc atggaagacg ctaattttcg ttccattacc cgccaactga
    40261 tgattaatga acaagcccga acacttaatc cagacttaaa tatcaacatc caggaatacg
    40321 ccgacctgtt aattgaacgc tttagcaacc gtaatgttgc acaccgtacc gggcaaatag
    40381 ctatggacgg ttcgcaaaag cttccccagc gagcgctgac gccctggctg aaattgcatc
    40441 agcaaaaaca aaacaatgct gttctgtcac tgctcgttgc tggttggttg cattatgtca
    40501 ttgatgctgt tgagaaaagc cagtctgtcg ctgatccaat gaatgaccaa cttcaggcgc
    40561 taataaagga acaacaagac gcatggcaac aggcgctcgc attactgcac cttagcgcca
    40621 tatttggtga tttaagcaac catcagccat ttataaatga aataaaaatc gcctttgcaa
    40681 atataaaaaa caaaggcatc aaggccacca tcagccaatt attatcggat gagcagaaat
    40741 gaaaacatta atttgtcagc agcctggcgt tatggaatat gtggaaaagg atattcccac
    40801 accagcagat aatgaagtgc tgttaaaaat caaagctgtg ggt
//
LOCUS       DAZFFE010000027        40779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFFE010000027 DAZFFE010000000
VERSION     DAZFFE010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.027"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(227..1084)
                     /gene="murI"
                     /locus_tag="ACHJ69_001696"
     CDS             complement(227..1084)
                     /gene="murI"
                     /locus_tag="ACHJ69_001696"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO5014698.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEVFIVERVVEIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLD"
     gene            complement(1029..2873)
                     /gene="btuB"
                     /locus_tag="ACHJ69_001697"
     CDS             complement(1029..2873)
                     /gene="btuB"
                     /locus_tag="ACHJ69_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO5014699.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDHPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDVWSGFVRGYGYDNRTNYDAYYSPGLPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNIGAG
                     VDWQKQSTAPGTAYVEDGYDQRNTGIYLTGLQQVGDFTFEGAGRSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGSYGNPNLNPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2938..3128)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3242..4342
                     /gene="trmA"
                     /locus_tag="ACHJ69_001698"
     CDS             3242..4342
                     /gene="trmA"
                     /locus_tag="ACHJ69_001698"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO5014700.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPILR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQQAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPIAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4382..4741)
                     /gene="yijD"
                     /locus_tag="ACHJ69_001699"
     CDS             complement(4382..4741)
                     /gene="yijD"
                     /locus_tag="ACHJ69_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO5014701.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4741..5391)
                     /gene="fabR"
                     /locus_tag="ACHJ69_001700"
     CDS             complement(4741..5391)
                     /gene="fabR"
                     /locus_tag="ACHJ69_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO5014702.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            complement(5495..6811)
                     /locus_tag="ACHJ69_001701"
     CDS             complement(5495..6811)
                     /locus_tag="ACHJ69_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HFO5014703.1"
                     /translation="MTAIVNETVRVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADHNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPIGCWLR
                     LSLENDVPEPVRNKKAATSESAFSLLLQHKATIVNGVLLAIGSTVATYISLFYYGTWA
                     AKYLAMPQHYSHAAMLLAGVVTFVGALLVGMLCDSVGRKKLILISRVMVLLCSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            complement(6854..8020)
                     /locus_tag="ACHJ69_001702"
     CDS             complement(6854..8020)
                     /locus_tag="ACHJ69_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HFO5014704.1"
                     /translation="MIEHYIRGFEEDLREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGVVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFSGTLRLIFQPAEEMINGGEIMVKEGLFDRFPCDVIFGM
                     HNMPGLPVGKFFFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGACMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
     gene            8350..9750
                     /gene="sthA"
                     /locus_tag="ACHJ69_001703"
     CDS             8350..9750
                     /gene="sthA"
                     /locus_tag="ACHJ69_001703"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO5014705.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEANAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(9733..10650)
                     /gene="oxyR"
                     /locus_tag="ACHJ69_001704"
     CDS             complement(9733..10650)
                     /gene="oxyR"
                     /locus_tag="ACHJ69_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO5014706.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(10902..12185)
                     /locus_tag="ACHJ69_001705"
     CDS             complement(10902..12185)
                     /locus_tag="ACHJ69_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5014707.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTEEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            complement(12252..13466)
                     /gene="manD"
                     /locus_tag="ACHJ69_001706"
     CDS             complement(12252..13466)
                     /gene="manD"
                     /locus_tag="ACHJ69_001706"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFO5014708.1"
                     /translation="MKIIAVDVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLADEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGRSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            complement(13964..15337)
                     /gene="argH"
                     /locus_tag="ACHJ69_001707"
     CDS             complement(13964..15337)
                     /gene="argH"
                     /locus_tag="ACHJ69_001707"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO5014709.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKALEDLPLSELQKFSQVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(15398..16174)
                     /gene="argB"
                     /locus_tag="ACHJ69_001708"
     CDS             complement(15398..16174)
                     /gene="argB"
                     /locus_tag="ACHJ69_001708"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO5014710.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(16182..17186)
                     /gene="argC"
                     /locus_tag="ACHJ69_001709"
     CDS             complement(16182..17186)
                     /gene="argC"
                     /locus_tag="ACHJ69_001709"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO5014711.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDVAFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVSQVQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            17340..18491
                     /gene="argE"
                     /locus_tag="ACHJ69_001710"
     CDS             17340..18491
                     /gene="argE"
                     /locus_tag="ACHJ69_001710"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO5014712.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLAKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYDAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            18793..21444
                     /gene="ppc"
                     /locus_tag="ACHJ69_001711"
     CDS             18793..21444
                     /gene="ppc"
                     /locus_tag="ACHJ69_001711"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO5014713.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKSGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            21627..23360
                     /gene="eptC"
                     /locus_tag="ACHJ69_001712"
     CDS             21627..23360
                     /gene="eptC"
                     /locus_tag="ACHJ69_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO5014714.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPEDQGKFDGNTEHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAT
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            23586..24437
                     /gene="yijO"
                     /locus_tag="ACHJ69_001713"
     CDS             23586..24437
                     /gene="yijO"
                     /locus_tag="ACHJ69_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO5014715.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPAPDLAYQVDFPRLE
                     IVLEGEFIDTGAGAALVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQSQEQQTARLIVTSLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(24424..24765)
                     /gene="frwD"
                     /locus_tag="ACHJ69_001714"
     CDS             complement(24424..24765)
                     /gene="frwD"
                     /locus_tag="ACHJ69_001714"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO5014716.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(24767..25645)
                     /gene="pflC"
                     /locus_tag="ACHJ69_001715"
     CDS             complement(24767..25645)
                     /gene="pflC"
                     /locus_tag="ACHJ69_001715"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO5014717.1"
                     /translation="MTSSAGQRISCNVVEMRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDAAQARDVVKMNLQRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(25611..27908)
                     /gene="pflD"
                     /locus_tag="ACHJ69_001716"
     CDS             complement(25611..27908)
                     /gene="pflD"
                     /locus_tag="ACHJ69_001716"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO5014718.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVIMRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQIHILRYAELAETMAASCT
                     DGQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMIFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(27959..28279)
                     /gene="frwB"
                     /locus_tag="ACHJ69_001717"
     CDS             complement(27959..28279)
                     /gene="frwB"
                     /locus_tag="ACHJ69_001717"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO5014719.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(28294..29373)
                     /gene="frwC"
                     /locus_tag="ACHJ69_001718"
     CDS             complement(28294..29373)
                     /gene="frwC"
                     /locus_tag="ACHJ69_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO5014720.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGVMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTEEKEDDLDLDFEIN"
     gene            29682..32183
                     /gene="ptsP"
                     /locus_tag="ACHJ69_001719"
     CDS             29682..32183
                     /gene="ptsP"
                     /locus_tag="ACHJ69_001719"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO5014721.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPISLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIAALTPWQHQTIYIDG
                     NAGAIVVEPGESVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIPLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLVQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            32195..32857
                     /gene="fsa"
                     /locus_tag="ACHJ69_001720"
     CDS             32195..32857
                     /gene="fsa"
                     /locus_tag="ACHJ69_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO5014722.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVKEAKHLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            32868..33971
                     /gene="gldA"
                     /locus_tag="ACHJ69_001721"
     CDS             32868..33971
                     /gene="gldA"
                     /locus_tag="ACHJ69_001721"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO5014723.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            34247..34864
                     /gene="yijF"
                     /locus_tag="ACHJ69_001722"
     CDS             34247..34864
                     /gene="yijF"
                     /locus_tag="ACHJ69_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO5014724.1"
                     /translation="MKASLALFSLLTLFTSYSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     AYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFAK"
     gene            complement(34891..35796)
                     /gene="yijE"
                     /locus_tag="ACHJ69_001723"
     CDS             complement(34891..35796)
                     /gene="yijE"
                     /locus_tag="ACHJ69_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO5014725.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(35890..38070)
                     /gene="katG"
                     /locus_tag="ACHJ69_001724"
     CDS             complement(35890..38070)
                     /gene="katG"
                     /locus_tag="ACHJ69_001724"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO5014726.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(38399..39289)
                     /gene="metF"
                     /locus_tag="ACHJ69_001725"
     CDS             complement(38399..39289)
                     /gene="metF"
                     /locus_tag="ACHJ69_001725"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO5014727.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPAELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLRRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(39671..>40779)
                     /locus_tag="ACHJ69_001726"
     CDS             complement(39671..>40779)
                     /locus_tag="ACHJ69_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO5014728.1"
                     /translation="TIIEKDGVKIGVIGLHGVFAFNDTVSAATRVGIEARDEIKWLQR
                     YIDEPKGKVDLTVALIHEGVPARQSSMGGTDVRRALDKDIQTASQVKGLDILITGHAH
                     VGTPEPIKVGNTLILSTDSGGIDVGKLVLDYKEKPHNFTVKNFELKTIYADEWKPDQQ
                     TKQVIDGWNKKLDEVVQQTVAQSPVELKRAYGESASLGNLAADALLAAAGKNTQLALT
                     NSGGIRNEIPAGAITMGGVISTFPFPNELVTMELTGKQLRSLMEHGASLSNGVLQVSK
                     GLEMKYDSSKPVGQRVITLTLNGKPIEDATVYHIATQSFLADGGDGFTAFTEGKARNT
                     TGGYYVYHAVVDYFKAGNTITDEQLNGMRVKDIK"
BASE COUNT        10208 a        11217 c        10008 g         9346 t
ORIGIN      
        1 ccgcttcgct ttttctcagc ggcgcggggt gtgcataata cgctttcccg ctacagagtc
       61 aagcatttat ttttgctttc tctgccggaa ttctcaggag aatcccgctg acccggcggc
      121 gtgtttgccg ttgttccgtg tcagtggtgg cgcattatag ggagttattc cggcctgaca
      181 agaggaaatt taaaataatt ttctgaccgc gcaacattca accaaatcaa tctaaaactg
      241 ccagtttttc gagcgtttcg aagccgtaac gctgtaaaac gggcaataat tgttcagctt
      301 ctggcgtcat tgccatacaa aatgcaatat tcgcatcggc agattttgca tccggggctt
      361 catgctctaa caaccaggcc gttcggcgag caatcgctgc gccagaatcc accagccggg
      421 ttccctctgg cagcacttgt aatagttctt cttgtagtag agggaaatgg gtgcaaccca
      481 atacaactgt atctggcggc tctttcattc ttaaccacgg gcgtaagata cgttttagcg
      541 catccagaga aacatcttcg ccatgcagct tcgcttcagc caactcaacc atctctgccg
      601 agcccagcat ttctatctgg cattcattag cgaaacgcgc gatcagctca tgagtataag
      661 aacgtttaac agttccgcgg gttgccagta atccgacaat gccatttgcc gtcagacgtg
      721 cagcaggttt aatcgccggc acgacaccaa caaccgggaa gtcgaacttt tcgcgtaatg
      781 caggaagtga aacggtactg gcggtgttgc aagcgaccac agccagcgca aggggataac
      841 gttcttgcac tgcggtgaca atttccacca ctcgctcaac aataaacact tcgctttttt
      901 cgccatacgg gaaagcgacg ttatcgaaag cataaatgta atggagatcc ggtaagagat
      961 gccggatctc gtcatagacc gacaacccac cgacgccgga gtcaaacacc agcacggtgg
     1021 gacgtggttc agaaggtgta gctgccagac aaggtgtatt cccgtcctgc agtttggtag
     1081 ccatagactg tctcataatc tttgtcgaac aggttggcta ttttaccacg aactgtcagg
     1141 tgagaggtga ccggatacgc aaccgcaaga tcccacaagc tcacaccgcc cattttaacg
     1201 gtttgataag gataagagga gtaatcctta tcatagcgag tgcctaaata ctgataagta
     1261 atcccccagt cgaagtcata caactgccag tcgagctggt atttcacctg ctgtttagca
     1321 cggcgtaaca acggcgtgtc agtaatcgca ttgcgcgcat caacataatc ataactcaca
     1381 gtatgcgtca gtggtccggt atcaaaatta gcggtcgcct cgacaccctt aatccgcgct
     1441 ttcccttcat tgtaatattt cagggtgtga tcatcataat cgatcaagtc actgacatcg
     1501 ttacgatatc cggaaatacg ccagttaacc ccagcagtta agccttcaaa cgcgccttcc
     1561 cattgtttgc ttttctcagg attcagattc ggattaccgt aggagccgta cagttgccca
     1621 agatttggtg ccttataaga tgttccgtag gaagcaataa agcgataacc ttcgatgaac
     1681 tcccaaccgg cgctggtttg ccaggtacca tgacgaccaa actgagaatt atcgtcgctg
     1741 cgtcctgcgc cttcaaaagt aaaatcgccg acttgctgca gcccagtcag atagatgccg
     1801 gtattacgtt gatcatatcc atcctcaaca taagctgtgc ccggtgccgt gctctgcttc
     1861 tgccagtcaa cacccgcacc aatattaccg tggccaatga tgatgttgtt tgcccactgg
     1921 acggtgtact gtttcatctc atcgagcgtc gcggacgaat cataacgacc ataatgcgga
     1981 tcgtagttgt aatctttgct atggctgtag ctggtaatca gttgtgattt aatcagttcg
     2041 ccgttatagc gcagtccggc atcccaactt tggctataga gtttacgggt atcgaccagt
     2101 ggcaagccgg gagaataata tgcgtcataa ttggtacgat tatcatagcc atagccgcgc
     2161 acaaagccgc tccagacatc agtaaaatta tgctccagcg cgccataaag cgttttactt
     2221 aaaaaaccat cgttatctgg ctgcgcttgc gttccggtat taccataggc gacaacatca
     2281 taaccatgag tatgggcata atcgcccaac agcgttaccc gtgtcttatc ccccagttgt
     2341 tgctgtgtag agacatcata gttctgataa ctattgcttc cccaccctgc tgaaatttcc
     2401 gttccggggt gatcgcgcgt cgtgatgata ttcacgaccc cgcctattgc atctgaacca
     2461 taaacagcgg agcgcggccc acggatatat tcaacacgct ggacaagcgc aatagggaac
     2521 tgactaagat cagcagaacc actcaccccc gccaaattca ggcggacgcc atcaattaac
     2581 accaacacat gactggcatt tgtaccgcga ataaaaatag atgagagctg acctgaaccg
     2641 ccgttttggg tgatatccac gcccggaaga cggcgcagca catcattgac cgaggtcgac
     2701 tgccagcggt cgatatcctg acgcgtcaca acagtagttg gcgcaagcac agtgctgcgc
     2761 ggttgttcaa aacggttagc agtaacgacg agagtatccg ggctggtatc ctgtgcccaa
     2821 gcggaaaatg ccgtgacgga acacgccgtc agcagcgaag cttttttaat cattgtaaag
     2881 catccacaat agaagaagga tgccgcaggt ttcatcaata ttacgcgatg atgagaacca
     2941 gatgcgacgt tggccggcag gtcttcgggc ttggaggggt atctaagata ctaagagatg
     3001 atgacttccc accggatggc agtgtccgca taacgcaatc atcgcacctt tccttaccgc
     3061 tgcgcgtcag ctccagattc gcactggatt ccctattaac tcacaggacc ggcaagtgga
     3121 tgctacaggt tgtaacaagt tactgtccag acgtagctca caaataggaa ttcatcaaga
     3181 tctggacatc tgatgagcaa tccctacaat cgccgcgtac tttaattttt caggatacat
     3241 catgaccccc gaacaccttc caacagaaca gtatgaagcg cagttagccg aaaaagtggt
     3301 acgtttgcaa agtatgatgg caccgttttc tgacctggtt ccggaagtgt ttcgctcgcc
     3361 ggtcagtcat tatcggatgc gtgcggagtt ccgcatctgg cacgatggcg atgacctgta
     3421 ccacatcatt ttcgatcaac aaaccaaaag tcgtatccgt gtggatagct tccccgccgc
     3481 cagtgaactt atcaaccagt tgatgacggc gatgattgcg ggtgtgcgta ataatcccat
     3541 tctgcgccac aagttgttcc agattgatta cctcactacg ctaagcaatc aagcggttgt
     3601 ttccttgctg taccataaaa agctggatga tgagtggcgt cagcaggcgg aagccctgcg
     3661 cgatgcactg cgcgcgcaga atctgaatgt gcatctgatt ggtcgggcaa cgaaaaccaa
     3721 aatcgcgctg gatcaggatt acatcgacga acgtctgccg atcgcgggga aagagatgat
     3781 ctaccgtcag gtagaaaaca gctttaccca accgaacgcg gcgatgaata ttcagatgct
     3841 ggaatgggcg ctggacgtaa ccaaaggctc aaaaggcgat ttactggagc tatactgcgg
     3901 caacggtaac ttttcattag cgctggcacg caattttgat cgggtattag ccaccgaaat
     3961 cgccaagccg tcagtcgccg ccgcgcaata caacattgcc gctaaccata ttgataacgt
     4021 acaaattatt cgtatggcgg cagaagaatt tactcaggcg atgaatggtg tgcgcgagtt
     4081 taaccgcctg caaggaatcg acttaaagag ttatcagtgc gaaaccattt ttgtcgaccc
     4141 accgcgcagc ggtctggaca gtgaaaccga gaaaatggtg caggcgtatc cacgtattct
     4201 gtatatctcc tgcaatccgg aaacgttatg caagaatctg gaaacattaa gccagacgca
     4261 caaggtcgaa cgtctggctc tatttgatca gttcccctac acgcaccata tggagtgcgg
     4321 cgtattactg accgcgaagt aaaacccatg ccggatgcgc ttgcatccgg catgataccg
     4381 attactcagc aacttcctgc ttacggttac gcatcttcgc gccaatccag aacaccataa
     4441 tgacggacag tactgccggg aagaagttag agccgatatc cggatattcc gcacgaacta
     4501 ccgtgctgta cagcagtacg ccgagaataa aacaagcagc tgccagaccc ggcaagccta
     4561 ccggcatagt gcgattaaga taacgttgat gcaggcagta aactgtcaac accagggaaa
     4621 taatcgggaa tacagaaaat ggtacaatgg agctaaacag cgctgcaaaa gtaccattga
     4681 tcgataagcc agcaaccaac gctagcagca gcgtacctct atcttgattt gcttgtttca
     4741 ttactcgtcc ttcacatttc ccggaataat tgcggttttc tcttgttcac ggcgatacca
     4801 gtaataagcc cctttcgaaa tcattcgcag ctgcagtacc agtcgctctt ctaattgccg
     4861 gcgttgctcg acgccgacat ccaacgcctc ggcccccgca ctgaagacaa tcgtcaccat
     4921 tgcttcggct tgcgcttcag taaacgcacg cggcatatgg ttttcgagtt ccagatagtc
     4981 cgcaagttcc gcaatgaagt gctgaatttc acgcgcaacg gcggcacgaa acgcagcgga
     5041 ggtgccggag cgttcccgca ataataaccg gaaggcgtta ggattattac cgatgaactc
     5101 cataaatgtg gagaccgagg tgcggatcac actcccgcct ttggcgatac gctgacgcgc
     5161 ctgacgcatg agttggcgta gcattaaacc gctctcatca accatggtca gacccagttc
     5221 gtctacgtcg cggaaatgcc gataaaaaga ggtgggagca atgcctgctt ctcgtgccac
     5281 ttcacgcaaa ctcaggctgg cgaagctgcg ctcagcactt aattggctaa atgcggcttc
     5341 caccagcgaa cggcgggttt tttctttttg ttgcgctctt acgcccatca cgatgtctga
     5401 atccttgcca aaagtactgc tggcactata ccagagaatg aacatactgg attgctccag
     5461 tgttgtgaat gaacggtaac gcaataaaac atatttactg caattctttg gccggttctt
     5521 ttacgtacaa caacgccagc atcgacagca acgtgcctcc ccccagatac cacgccggag
     5581 ccgtcagact atccagcaac acgatggatt gcgtagcgaa atattgagca aaaccgccaa
     5641 agattgccac accaatgcta taaaccagcg cgaaacctaa tgcccgaatc cgtttcggca
     5701 acagttcaga gatcaacaac atcactggca caccgccaag cgtggtaaag ctgaccatca
     5761 cgaaaaccac cgtcagcaac atgccagggc ttgggtaatt caccaacagc cagaatgacg
     5821 gccagctaca gagaagcacc atcacacggg agattaaaat cagcttttta cgcccaaccg
     5881 aatcacatag catccccacc aacagcgcgc ccacaaacgt aacaacgccc gccagtaaca
     5941 tcgctgcatg agaataatgt tgaggcatcg ccagatactt cgccgcccag gtgccgtaat
     6001 aaaagaggga gatataggtt gcgaccgtgc taccaatagc gagtaaaaca ccattaacga
     6061 tagtcgcctt atgttgcagg agcagagaaa acgcgctttc gctggtagca gcttttttat
     6121 tgcgcactgg ctcaggtacg tcattttcca ggctcagacg caaccagcaa ccgataggtg
     6181 ctaataacac gccaataaaa aacggcacac gccagcccca ttctgccatt acggtttctg
     6241 aacctgtggc gaacggtagc catgcgctca gacctaaagc aacaacccca ccgaaggtcg
     6301 ttgccagccc ttgggtcgcc agtgaccaac tgctgtaaaa gccgcgacga tttgccggag
     6361 ctgactccac cagtagcgac attgaagccc cgacttcacc acctgccgca acgccctgaa
     6421 ttagacgcgc cagcaccagc gttgcagttc cccaatagcc cgccgtttca tacgtcggtg
     6481 cgatgccgat catcagcgta ccgaggctca tcagagcaat agtgaacacc atcgccggtt
     6541 tacgcccgaa gcgatcggcc caggcaccaa cgataatccc accgagcgga cgcataaaaa
     6601 agctcacacc aaacgttgca aatgccagta acagcgcgac gttatgatcg gcactgggtg
     6661 ggaagaaaag cgtactgata tagaccacaa aaaagttgta aacgccaaaa tcaaaaaatt
     6721 cgagcgcatt gccaaacgcc gaggcaaaga tcacccgcct gccgctgggc tttttcttca
     6781 ctctcacggt ttcatttacg atcgccgtca ttccatgttc cttgccaaaa agtgttgttg
     6841 tgtgttgttt tatttattgc agccaggctt caaccagtgc gccccagtag ctgcttgccg
     6901 ggaccaggct tgcatcgtta aagtcgtaac cggggttatg caccatgcag gcaccttccc
     6961 cgacatcgcc attaccgaat aacaggaaac cgcctttggg ttgggcttcc agcatgaaag
     7021 agaaatcttc actgcccatc aggggtttga tgccaaactc agcccgatct tcaccaaatg
     7081 ttttgcacgc cacctgccag gcgaagcgcg ccatctcctc gtcatttact aataccggtg
     7141 taccgttaac atgcgtaacc tcagccgtag cgccaaagct ggcagcctgg gcttgtgcaa
     7201 gagcaggaat acgggtcaga agaagttgtc gggtatcgcg gctaagagag cggacactga
     7261 ttttcatctc agcactgtca ggaatgacgt tagccgcctc acccgcaaca atgctgccga
     7321 cggtaattac cgccgcttcc agcggatcga cattgcgcga cacaatgctt tgtaatgcgg
     7381 tggtgatatg tgcggcaacc agcactggat cgatggcttt gtgcgggatc gcaccgtgtc
     7441 cgccacaacc acgaaccgta atatggaact gatccattga cgccatcagc gccccaggct
     7501 ggaagaaaaa cttgccgacc ggcaaacccg gcatgttgtg catcccgaaa atgacatcgc
     7561 aggggaaacg atcaaaaagc ccctctttaa ccatgatctc accgccgtta atcatctctt
     7621 ccgcaggctg gaagataagg cgtaacgtgc cgctaaaacg gcgggtttcg gcaaaataac
     7681 gcgcagcccc cagcagtatg gtggtatgcc cgtcatgccc gcaggcgtgc attaaaccag
     7741 gatgtttact ggcccagggc ttgccactat tttcataaat cggcaacgca tccatatccg
     7801 cccgcaggcc aatacttttc tctccgtcgc ccacttttaa cgtggctacc acgccggttg
     7861 tcgccagccc ttgctcaacc tcatagcccc actggcgcag tttttccgca accaaagcac
     7921 tggttttaaa ttcttgtaat cccagctccg ggttttcgtg gatctgatgg cggatctcgc
     7981 gaaggtcttc ttcaaaccca cgaatatagt gttcgatcat cggctattcc tttattcact
     8041 tataaacagg ctagttaacg gattttattg atggtataaa taaaaataac tcatcaatat
     8101 aaaatatcta tatatttgtt ttataagaag atttcataaa gacaataaca aaaaagcacg
     8161 cttcggcaag ataaaactat cagccataag aaaaagcgat taatagtgaa taaataaaat
     8221 aagaaggagg aagacaaagc aaaggccgct cacgatatag ccagataaat gacggggatc
     8281 aattggctta cccgcgataa aatgttacca ttctgttgct tttatgtata agaacaggta
     8341 agccctacca tgccacattc ctacgattac gatgccatag taataggttc cggccccggc
     8401 ggcgaaggcg ctgcgatggg cctggttaag caaggtgcgc gcgtcgcagt tatcgagcgt
     8461 tatcaaaatg ttggcggcgg ttgcacccac tggggcacca tcccgtcgaa agctctccgt
     8521 cacgccgtca gccgcattat agaattcaat caaaacccac tatacagcga ccattcccga
     8581 ctgctccgct cttcttttgc cgatatcctt aaccatgccg ataacgtgat taatcaacaa
     8641 acgcgcatgc gtcagggatt ttacgaacgt aatcactgtg aaatattgca ggggaacgct
     8701 cgctttgttg atgagcatac gttggcgctg gattgcccgg acggcagcgt cgaaacacta
     8761 actgctgaaa aatttgttat tgcctgcggt tctcgtccat atcatccaac agatgttgat
     8821 ttcacccatc cacgcattta tgacagcgac tcaattctta gcatgcacca cgaaccgcgc
     8881 catgtactta tttacggtgc tggagtgatc ggctgtgaat atgcgtcgat cttccgcggt
     8941 atggatgtaa aagtggatct gatcaacacc cgcgatcgtc tgctggcgtt tctcgatcaa
     9001 gagatgtcag attctctctc ctatcacttc tggaacagtg gcgtagtgat tcgtcacaac
     9061 gaagagtacg agaagatcga aggctgtgac gacggtgtga tcatgcatct gaagtcgggt
     9121 aaaaaactga aagctgactg tctgctctat gccaacggtc gcaccggtaa taccgattcg
     9181 ctggcgttac agaacattgg gctggaaact gacagtcgcg gacagctgaa ggtcaacagc
     9241 atgtatcaga ccgcacagcc gcacgtttac gcggtgggcg acgtgattgg ttatccgagc
     9301 ctggcgtcgg cagcctatga ccaggggcgc attgccgcgc aggcgctggt gaaaggtgaa
     9361 gccaacgcac atctgattga agatatccct accggcattt acaccatccc ggaaatcagc
     9421 tctgtaggca aaaccgaaca gcagctgacc gcgatgaaag tgccatatga agtgggccgc
     9481 gcccagttta aacatctggc acgtgcacaa atcgtcggca tgaacgtggg cacgctgaaa
     9541 attttgttcc atcgggaaac aaaagagatt ctgggtattc actgctttgg cgagcgcgct
     9601 gccgaaatta ttcatatcgg tcaggcgatt atggaacaga aaggtggcgg caacactatt
     9661 gagtacttcg tcaacaccac ctttaactac ccgacgatgg cggaagccta tcgggtagct
     9721 gcgctaaatg gcttaaaccg cctgttttaa cactttatcg aaatggccat ccattcttgc
     9781 gcggatggcc tctgccagct gctcatagcg gctgcgcagc ggtgagccag gacgataaac
     9841 caggccaata gtgcggcgtg gttccggctt aatgcacggc agataaacaa ccccatcgcg
     9901 tttgcgttcc ggcggcacag ccagcgcagg cagtaaagtg atcccgctac ctgccgccac
     9961 catgttgcgc agagtttcca ggctggtcgc gcggaagtgt gtatcttcat ccgccccggc
    10021 ttcaaaacag aaacccattg cctgatcgcg caaacagtga ccatcttcca gcatcagcag
    10081 tttttcccct gccagatcgg ccatcggtac gcattcgcgg ttcgcccacg ggtggtcttc
    10141 atagatagcc agcaacattg gctcatcaaa caacggcact tcaatgaatg cttcgctctc
    10201 tttcaccagc gcgaggatca cgcaatcgag tttgccgctg tccagttgcg ccagtaactg
    10261 gtgggtctgt gcttcatgca gatacatttc cagctttgga aaggtctggt gcagcatcgg
    10321 gataatatgc ggtagcaggt acggtccaac cgtgggaatc aaaccaatat gcagcggccc
    10381 ggacatcgtc tcgccctgct ggcttgccat ctctttaagg actttcacct cacgcagtac
    10441 ggtacgcgcc tgatccacca gcagcattcc cgcctgggtg aacaacactt tacggctggt
    10501 ccgctccagc aacatcacgc ccaactcatc ttccagctta cgaatttgcc cgctaagcgt
    10561 cggctggcta acgtggcagg aatctgccgc acgccgaaaa tggcggtgtt cggccaatgc
    10621 caccaggtac tcaagatcac gaatattcat tatccatcct ccattgccac gatagttcgc
    10681 ggcgataggt agcatagcaa cgaacgatta tccctatcaa gcattctgac tgataattgc
    10741 tcacagaaac ggagcggcac ctcttttaac ccttgaagtc actgcccgtt tcgagagttt
    10801 ctcaactcga ataactaaag ccaacgtgaa cttttgcgga tctccaggat ccgctttttt
    10861 ttattctaca ggcccacagc aagagagcct gatagtaacg gttactcaat ccgatctttg
    10921 acaataaaga agtaacacag cgccccggtg aacgtcacac aggccgcgat aaccagcgcc
    10981 agattaaacg agtgtgtggt atccacgacc caaccggtta cgatcggagc aaaggaagca
    11041 aaaacaaaac tgccaaagtt ttgaatcccg gcgatggaag ccacttttgt ttctgacacc
    11101 atcacctgca ccagccccca tgcagatgtt cccgcgaaat gcacgcagaa cagcgccatt
    11161 gagataaacg ctaccgcctg ggctggcgag gaagattgca cgaccagcaa cgtgcctaat
    11221 gccgacatca tcaaaccgca gacaatcgcc gttttacgcg tcttcgccag gtcatagcct
    11281 tttttcgcca gccgatcgac aacaataccg ttaacccaca tcccgaccgc cgccgcgagg
    11341 aaaggaattg ccgctaccca accggttttc gccagactga aaccttgttc ggcctgcaaa
    11401 taaccgggta accacgcgat gtagagccaa ccggtataat tgacgccaga gaaacctaaa
    11461 atcatccccc aggttgtccg atgcttaaac agcgccagcc actcgctaaa ttgtaactgt
    11521 ggacgcggtt taaccggcgc ggaaagatat gtccgttctt cctcggtcag gacaaactgc
    11581 gcccggttgc gataccacgc gtaccaacag atcccaacca gaatccctgc tacgccgatg
    11641 atgacaaaca tcgtccgcca gccccatgcc agttgcatca atacaagagc aggaggcgcg
    11701 atagcctgac cgataacggt agatgagtta aaaatcccca gcgccgtgcc gcgttctttt
    11761 tgcgcatacc agtcggtgat cgactttact cccgcaggca taaatggcgc ttcgccaata
    11821 cccagaccga tacgcattaa aataaagtgg ctaaaagaat taaccattcc tgttaatgcc
    11881 tgcattaatg accagaatat taagcctgcg cctaacacta ttcgtgggcc aaagcggtct
    11941 agcaaaatac cggaaggaag ttgtgaaaaa ccataagaca gagaaaatgc agaaagcaaa
    12001 acaccaaact ctgtcgccga taatcctaac tcgccacgaa tcgcttcacc tgccacgctc
    12061 aatgatgaac ggtcgagaaa attaacgatc cccgccataa ataataaaac cagggtcact
    12121 gtctgaatac gacgaatccg ttgcggttta ttacccgcaa cactggttga cacatcaacg
    12181 tccattttta gctccttgag cggaattaat cgatttgatg aatattcaga aaataattaa
    12241 gcgaaccgct attaccagtt ccacaatgtg ccatcttcca gtcgtgcgac ggggagatac
    12301 gccgggtcat acggatattt cgccgccagt ttctcatcga aagaaatacc cagccccggt
    12361 tcatcgcctg ggtgcatata accttcatcg aagcgccagc tgtgcgggaa gacttcaagc
    12421 atttgttcgg aatagcccat gtactcctga acgccaaagt ttggcaccca gaggtcaaaa
    12481 tgcagcgccg ccgcatgaca aacaggcgat aaatctgaag ggccatgcga accggtacgt
    12541 acctgataaa gcgatgcaaa atcggcaatc cgtcgcatcc cggtaatgcc gcctgcatgg
    12601 gtaatcgtcg tgcgaatata gtcaatcagc tgctcttcga ttaactgttt gcagtcccag
    12661 atactgttaa acacttcacc gaccgcaatc ggcgtcacgg tatgctggcg aatcagacga
    12721 aaacattcct ggttctcggc aggcgtcgga tcttccatcc agaacaggcg gtaatcctca
    12781 atgctcctcc caaagcgcgc cgcttcgatg ggggtcaggc gatggtgcat gtcatgcaac
    12841 agatgttcgt taaaaccgaa cttattacgc accgcttcaa ataacttcgg cgtaaaatcg
    12901 agatactttt ctgaagacca caactgctct tctggccaca ggcctttggt tgctggttca
    12961 tccgccagtc ctttgccctt cgccagacca taggtggttt gcatacccgg tacaccacac
    13021 tgcacacgaa tcgccttaaa gcccatctgc tggtgctttg catagtcttc aaggacttca
    13081 tcaatcgtcc gcccggtggt gtggcaataa accatcacgc cttcacgaga agcgccacca
    13141 agcagttgat acaatggcat atttgccgct ttagctttga tatcccacaa cgccatatca
    13201 atggcagaaa tggccgacat cgtgacggga ccacgtcgcc agtaagctcc tttataaaag
    13261 aattgccaga tatcttcgat acggctggca tcgcggccaa ttaattgagg acaaagatga
    13321 tctttcagat aagaagcgac cgacaattcg cgaccattta acgtggcatc tcctaatcca
    13381 caaagtccgc tttcagtggt tattttaagc gtaacgaaat tacgcccggg acaagtaaca
    13441 aaaacatcca cagcaataat tttcattatt gcaatctcct gtattgtaaa tagacacttc
    13501 aaacttaatt ttacgcctac taccatacaa gagagaaaaa gtgattattc aaccgtgatc
    13561 acattaaaaa taaaaatttc taccgaggag aaaaaacacc ctcaaaaacc acctgaataa
    13621 ttagccatca tcgttaaaac aacacgttaa ataatcacat aacaaactcc attaatcatt
    13681 aaaaatgaac acagcaatag aaatgaatta tattcaatta attaaagtat gtattggctg
    13741 atgtattgaa aatataattt ttatggggag agtgaaggta aagaagacgc aagaataaaa
    13801 aggaatgtga caaataatgg ttctggttgg gcgttaaatt taaggtcacc agaatactga
    13861 caaatttctg cctgatgcgg cgtaaacgcc ttatccgcct acgaagtgtc acactttgta
    13921 ggcgtggtaa gctccccatt ttcaggggag caatataaac aaattacccc aaccgcgcct
    13981 gcgcaaaagc aatcgcctgc gccacctgtt gcggtgagac accgcctttt gctgcacgct
    14041 tgtcgaggca cgattgcagc gacagaatcg gatagacatc ttcgccaatc acctggctga
    14101 atttctgcaa ctcactgagc ggcagatctt ccagcgcttt gccctgacga atggcttcca
    14161 ccaccgcttc acccacaata tggtgcgcct cgcggaacgg tacgcctttc gccaccagat
    14221 aatccgccag ttcagtagcg ttggcgtaac cctgctgcgc cgcttcctgg caacgcggac
    14281 gtttcacctg aatgccgtcc agcaccagcg ccgccatatg caggcagtcc agccaggtat
    14341 cgagcgcgtc gaacagacct tctttgtctt cctgcatatc tttgttgtaa gccagcggca
    14401 aacctttcag cgtcatcatc atgccggtta acgccccctg cacccgaccg catttaccgc
    14461 gaatcagctc cagcgcatcc gggtttttct tctgcggcat taatgatgaa ccggaagtca
    14521 cacggtcaga aagctccaca aaccccgctt cgccggtgtt aaagaaaatc agatcttcag
    14581 caaaacgcga cagatgcacc atgccgatag cggcagcaga aagcagttcc aacacatggt
    14641 cacggtcaga aacgctgtcg agactgttac gggtcgccga agcaaagccc agccagcctg
    14701 ctaactgttc acggtcgatt tcataggccg ttcctgccag tgcgccacag cctagcgggc
    14761 tgacatccag acgcttaagc gcatcctgca aacggctttc atcacgcgcc agcatctcaa
    14821 cgtaggccag gcaccagtgc gcgaacgtca ccggctgggc gcgttgcagg tgggtgtaac
    14881 ccggcatcac cgcgtcctga ttgttctgcg cggtctcgac cagagcggat tgtaactgcc
    14941 ggttagccgt cagcaactcg ctaacggtat ctttgcacca cagtttcagg tcagttgcta
    15001 cctgatcatt acggctacgc ccggtatgca gttttttgcc taactgaccc actttgtcga
    15061 tcagtttgcc ttccacccag ctatggatat cttcggcgtc gctttcaagg atttgttgtg
    15121 gcctggcgcg aacatcttcc agcagcacgt tcagcgcctc ttccagttgc gcctgctctt
    15181 ctgcggttaa cacgccgacc gttaccaggg ctttggacca ggccacagag ccaacaatat
    15241 cctgctccgc cagacggtaa tcaaagcgca gtgagtcgtt gaattgtttg aaccgttgat
    15301 ctgctgcctg ggtaaaacgc ccgccccaaa gtgccataac tctgtttcct tattttgaaa
    15361 ttcaatgccg gaaagctgcg cctccggcca acaaaactta agctaaaatc cgcgtaccca
    15421 tcggcatacc gttaaacagt gccggaagct gttccgcatg acgccaggag gcgatatcta
    15481 ccggacggcc caacgtgcgc gcagcatcca gcgccgcgtt cactttcaca atcatgccgt
    15541 cagtaataat gccctgctca atcagttgtt ctgctttcgc ggcggtcatt tcggcaatac
    15601 gttgcccttt gccgtcgaga atgccgctga cgtcagagag caaaatcaga tccgcgccca
    15661 gcgttgccgc cagcgccgtc gccgcctggt cggcattgac attcatcagt tgcccttcgt
    15721 ctgttacgcc aatggagctg accaccggca gataaccgtt ctccagcaaa ctgttgataa
    15781 gcttaggcga acctggctgc gccagtccaa catggcctaa ctcttcatca agctgggtca
    15841 ctttgacgct gtcgccgtca ccgagaaaca aacctacggc cgcaatctga tgtttcttcg
    15901 cccatgccag cagggtttta tttgccgttc ccgccagtgc tccggtgata atgtctatct
    15961 gatccgcagg cgtcacccgc aggccatttt tctttttcac cggcagattc agccctttca
    16021 tcagctcatc caccacgcaa ccgccgccgt gcacaatcac cagcggacgc tgatgtgact
    16081 cgcgataatt caccagtgcg ctaaacagac gttccagcgc ctcttcacta tccagcagta
    16141 cgccacccag tttgataatt aatggattca tcattgcacc cttaaataag agactgcgtt
    16201 tccgcatagc caaaacgaat attggcgcac tgcaccgctt gtgccgccgc gcctttcagt
    16261 aagttgtctt cggtcgccac aataatcaga tgctcgccct gaacggcaaa accgatatcg
    16321 cagaatggca gtccaacgac atttttcagc gccggaacgc ctttgtcata cagccgcacc
    16381 agtggtttat gggcatacgc ctgctgtaac gcttgcgcca cttgcacctg gctcacaccc
    16441 ggtttcaggc ggcaggtaat ggtttcgaga atgccgcgcg ggaaattgcc cagatgcggg
    16501 gtgaagataa cgtcagcacc gaggtgtgtg gcgatctctg gttgatggcg atgggtaaag
    16561 acgccatacg gttgcaggct aacttcacaa aaactatttg aaatcgctgc cttacgtcct
    16621 gcaccgctca cgccgctggt ggcgttgatc accggccact gattgaggtc aagaagatcg
    16681 gcatcaatca acggtttcag cgccagctgt gctgccgtcg gataacagcc tggcacggca
    16741 atcaagttcg cttcttttaa tttattaccg caccactccg ccagaccgta ggctgcctgt
    16801 tccagcagtt ccgggtattg atgggtaaag ccgtaatatt tttcatagaa ggcgacatcg
    16861 ttaacacgaa acgcgccgga aaggtcgaac accacacagc ctgcttcaag aaattgcggc
    16921 gctaaatcgt ggctgacttc atgggcggtg gcaagaaaca ccacgtccac ccctgggcta
    16981 aactcgctga tatccgacat cggctgcaac ggcagatcaa cgatgccttt tagctgcgga
    17041 tgcaaatcgg agattaactt tcccgcatca ttgctttgcg ctgaaacagt caaagcggtt
    17101 atgttcatat gcggatggcg atttacatag gtcactagct ctgcgccagc gtagccgctg
    17161 gcacccacaa tcagcgtatt caacatcggg gctattcacc ttcttatgtc tggttgccag
    17221 gttaaacgta aaacattcac cttacggctg gtgggtttta ttacgctcaa cgttagtgta
    17281 tttttattca taaatactgc atgaatattg atactatcat gaccagaggt gtgtcaacaa
    17341 tgaaaaacaa attaccgcca tttatcgaga tttaccgtgc tctgattgcc acaccttcaa
    17401 taagcgccac ggaagaggca ctcgatcaaa gcaatgcaga tttaatcact ctgctggcgg
    17461 actggtttaa ggatttaggc ttcaatgtgg aagtacagcc tgtgccagga actcgcaaca
    17521 aatttaatat gctggccagt tgcggacagg gggctggcgg tttgttgctg gcggggcata
    17581 ccgatacggt gccatttgat gacggacgct ggacgcgcga tccatttacg ttgacggagc
    17641 atgacggcaa gctttacggc ttaggcaccg ccgacatgaa aggctttttt gcgtttatcc
    17701 ttgatgcgct acgcgatgtc gacgtcacga agcttgcaaa accgctctac attctggcga
    17761 ctgccgatga agaaaccagt atggccggag cgcgttattt tgccgaaact accgccctgc
    17821 gcccggattg cgccatcatt ggcgagccaa cgtcgctgca accggtacgc gcgcataaag
    17881 gtcatatctc taacgccatc cgtattcagg gccagtcggg ccactccagc gatccagcac
    17941 gcggagttaa cgctatcgaa ctgatgcacg acgccatagg gcatattttg caattgcgcg
    18001 ataacctgaa agaacgttat cactacgacg cgtttaccgt gccataccct acgctcaacc
    18061 tcgggcatat ccacggtggc gacgcttcta accgtatttg tgcttgctgt gagttgcata
    18121 tggatattcg tccgctgcca ggcatgacac tcaatgaact taatggtttg ctcaacgatg
    18181 cattggctcc agtgagcgag cgctggccgg gtcgtctgac ggtcgacgag ctgcatccgc
    18241 cgatccctgg ctatgaatgc ccaccgaatc atcaactggt tgaagtggtt gagaaattgc
    18301 tcggagcaaa gaccgaagtg gtgaattact gtaccgaagc gccgtttatt caaacgttat
    18361 gcccgacgct ggttctgggg cctggctcaa ttaatcaggc tcatcagcct gatgaatatc
    18421 tggagacgag gtttatcaag cccacccgcg aactgatcac ccaggtgatt caccattttt
    18481 gctggcatta aaacgtaggc caaacaatgt cggatgcgat gcttgcgcat cttatccgac
    18541 ctacaccttt ggtgttactc gaggcgattt tttaacattc ccataagtta cgcttattta
    18601 aagcgtcatg aatttaatgg cgtaaattcc tgctatttat tcgtttgctg aagcaatttc
    18661 gcagcatttg acgtcaccgc ttttacgtgg ctttataaaa gacgacgaaa agcaaagccc
    18721 gagcatattc gcgccaatgc gacgtgaagg atacagggct atcaaacgat aagatggggt
    18781 gtctggggta atatgaacga acaatattcc gcattgcgta gtaatgtcag tatgctcggc
    18841 aaagtgctgg gagaaaccat caaggatgcg ttgggagaac acattcttga acgcgtagaa
    18901 actatccgta agttgtccaa atcttcacgc gctggcaatg atgctaaccg ccaggagttg
    18961 ctcaccacct tacaaaattt gtcgaacgac gagctgctgc ccgttgcgcg tgcgtttagt
    19021 cagtttctga acctggccaa caccgccgag caataccaca gcatttcgcc gaaaggcgaa
    19081 gctgccagca acccggaagt gatcgcccgc accctgcgca aactgaaaaa ccagccggaa
    19141 ctgagcgaag acaccataaa aaaagcagtg gaatcgctgt cgctggaact ggtcctcacg
    19201 gctcacccaa ccgaaattac ccgtcgtaca ctgatccaca aaatggtgga agtgaacgcc
    19261 tgcttaaaac agctcgataa caaagatatc gccgactacg aacataacca gctgatgcgc
    19321 cgcctgcgcc agttgatcgc ccagtcatgg cataccgatg aaatccgtaa actgcgtcca
    19381 agcccggtag atgaagccaa atggggcttt gccgtagtag aaaacagcct gtggcaaggc
    19441 gtaccgaatt acctgcgcga actgaacgaa caactggaag agaatctcgg ctacaaactg
    19501 cccgtcgaat ttgttccggt ccgttttacc tcgtggatgg gcggtgaccg cgacggcaac
    19561 ccgaacgtca ctgccgatat cacccgccac gtcctgctgc tcagtcgctg gaaagccacc
    19621 gatctgttcc tgaaagatat tcaggtgctg gtttctgaac tgtcgatggt tgaagcgacc
    19681 cctgaactgc tggcgctggt tggcgaagaa ggtgccgcag aaccgtatcg ctatctgatg
    19741 aaaaacctgc gttctcgcct gatggcgaca caggcatggc tggaagcgcg cctgaaaggc
    19801 gaagaactgc caaaaccaga aggcctgctg acacaaaacg aagaactgtg ggaaccgctc
    19861 tacgcttgct accagtcact tcaggcgtgt ggcatgggta ttatcgccaa cggcgatctg
    19921 ctcgacaccc tgcgccgcgt gaaatgtttc ggcgtaccgc tggtccgtat tgatatccgt
    19981 caggagagca cccgccatac cgaagcgctg ggcgagctga cccgctacct cggtatcggc
    20041 gactacgaga gctggtcaga agccgacaaa caggcgttcc tgatccgcga actgaactcc
    20101 aaacgtccgc ttctaccgcg caactggcaa ccaagcgccg aaacgcgcga agtgctcgat
    20161 acctgccagg tgattgccga agcgccgcaa ggctccattg ccgcctacgt gatctcgatg
    20221 gcgaaaacgc cgtccgacgt gctggctgtc cacctgctgc tgaaagaagc gggtatcggg
    20281 tttgcgatgc cggttgctcc gctgtttgaa accctcgatg acctgaacaa cgccaacgat
    20341 gtcatgaccc agctgctgaa tatcgactgg tatcgcggcc tgattcaggg caaacagatg
    20401 gtgatgattg gctattccga ctcagcaaaa gatgcgggcg tgatggcagc ttcctgggcg
    20461 caataccagg cacaggatgc attaatcaaa acctgcgaaa aagcgggtat tgagctaacg
    20521 ttgttccacg gtcgcggcgg ttccattggt cgcggcggcg cacctgccca tgcggcgctg
    20581 ctgtcacaac caccaggaag cctgaaaagc ggcctgcgcg tgaccgaaca gggcgagatg
    20641 atccgcttta aatatggtct gccagaaatc accgtcagca gcctgtcgct gtacaccggg
    20701 gcgattctgg aagccaacct gctgccaccg cctgagccga aagagagctg gcgtcgcatt
    20761 atggatgaac tgtcagtcat ctcctgcgat ctctaccgtg gctacgtacg tgaaaacaaa
    20821 gattttgtgc cttacttccg ctccgctacg ccggaacaag aattgggtaa actgccgttg
    20881 ggttcacgtc cggcgaaacg tcgcccaacc ggcggcgtcg agtcactgcg cgctattccg
    20941 tggatttttg cctggacaca aaaccgcctg atgctccccg cctggctggg cgcgggtacg
    21001 gcgctacaaa aagtggtgga ggatggtaaa cagagcgagc tggaagccat gtgccgcgat
    21061 tggccattct tctcgacgcg tctcggcatg ctggagatgg tcttcgccaa agcagacctg
    21121 tggctggcgg aatactatga ccaacgcctg gtagacaaag cactgtggcc gttaggtaaa
    21181 gagttacgca atctgcaaga agaagacatc aaagtggtgc tggcgattgc caacgattct
    21241 cacctgatgg ccgatctgcc gtggattgca gagtctattc agctacggaa tatttacacc
    21301 gacccgctga acgtattgca ggccgagttg ctgcaccgct cccgccaggc agaaaaagaa
    21361 ggccaggaac cggatcctcg cgtcgagcag gcgttaatgg tcactattgc cgggattgcg
    21421 gcaggtatgc gtaataccgg ctaatcttcc tttcctgcaa accctcgtgc ttttgcgcga
    21481 gggttttctg aaatacttct gttctaacac cctcgttttc aatatatttc tgtctgcatt
    21541 ttattcaaaa tctggatatc ccttcagata tccttaagga attgtcgtta cattcggcga
    21601 tatttttttc aagacaggtt cttactatgc attccacaga agtccaggct aaaccccttt
    21661 ttagctggaa agccctgggt tgggcactgc tctacttttg gtttttctct actctgctac
    21721 aggccattat ttacatcagt ggttatagtg gcactaacgg cattcgcgac tcgctgttat
    21781 tcagttcgct gtggttgatc ccggtattcc tctttccgaa gcggattaaa attattgccg
    21841 cagtgatcgg cgtggtgcta tgggcggcct ctctggcggc gctgtgctat tacgtcatct
    21901 acggtcagga gttctcgcag agcgttctgt ttgtgatgtt cgaaaccaac accaacgaag
    21961 ccagcgagta tttaagccag tatttcagcc tgaaaattgt gcttatcgcg ctggcctata
    22021 cggcggtggc agttctgctg tggacacgcc tgcgcccggt ctatattcca aagccgtggc
    22081 gttatgttgt ctcttttgcc ctgctttatg gcttgattct gcatccgatc gccatgaata
    22141 cgtttatcaa gaacaagccg tttgagaaaa cgttggataa cctggcatcg cgtatggagc
    22201 ctgccgcacc gtggcaattc ctgaccggct attatcagta tcgtcagcaa ctaaactcgc
    22261 taacaaagct actgaatgaa aataatgcct tgccgccgct ggctaatttc aaagatgaat
    22321 cgggtaacga accgcgcacc ttagtgctgg taattggcga atcgacccag cgtggacgca
    22381 tgagtctgta cggttatccg cgtgaaacca cgccggagct ggatgcgctg cataaaaccg
    22441 atccgaatct gaccgtgttt aataacgtgg ttacgtctcg tccgtacacc attgaaatcc
    22501 tgcaacaggc gctgaccttt gccaatgaaa agaacccgga tctgtatctg acgcagccgt
    22561 cgctgatgaa catgatgaaa caggcgggtt ataaaacctt ctggatcacc aaccagcaga
    22621 cgatgaccgc ccgcaatacc atgctgacgg tattttcgcg ccagaccgat aagcagtact
    22681 acatgaacca gcaacgtacg cagagtgcgc gtgaatacga caccaacgtg ctgaagccgt
    22741 tccaggaagt gctgaaggac cctgcgccga agaaactgat catcgttcat ctgctgggta
    22801 cgcatatcaa gtacaagtac cgttatccgg aagatcaggg caaatttgac ggtaatacag
    22861 agcatgttcc accgggatta aacgcagaag agctggagtc atataatgat tatgacaatg
    22921 ctaacctgta caacgatcac gtggttgcca gcctgattaa agactttaaa gcgaccgatc
    22981 cgaacggttt cctggtttat ttctctgacc acggtgaaga ggtttacgac acgccgccgc
    23041 acaaaactca ggggcgtaat gaagacaacc cgacgcgtca tatgtacacc attccgttcc
    23101 tgctgtggac gtcagaaaaa tggcaagcga ctcatccgcg tgatttctca caggatgttg
    23161 atcgtaaata cagcctggcg gaactgatcc acacctggtc agatttggcg ggcttatctt
    23221 acgacggtta cgatccaacc cgttcagtgg tgaatccgca gttcaaagaa actacccgct
    23281 ggattggtaa cccgtataag aaaaacgcac tgatcgatta cgacactctg ccgtatggcg
    23341 atcaggtggg taatcagtaa ttatcagcca gatgttattt gccggatgcg acgcttgacg
    23401 cgtcttatcc ggcctacaga tcgcggcggt aggccggata aggcgtttac gccgcatccg
    23461 gcaatacaag tcgactgaaa ctgccgcaat gtttacatct cactcccatc aggccacact
    23521 tttcaggtgg ccttttttca taatgccagt tactgttttc ccgtctacat acctaaggga
    23581 ataacatgta tcacgacgtc agctatctgc tctcccgcct gatcaacggc ccgctgtccc
    23641 tgcgccagat ttactttgcc agcagcaacg gcccggcgcc cgatcttgcg tatcaggtcg
    23701 attttccacg gctggaaatt gtgctggaag gtgagtttat tgataccggc gctggagcag
    23761 cgttagttcc cggcgatgtg ctgtacgtcc ctgctggtgg ctggaatttt ccacaatggc
    23821 aagcccccgc caccaccttt agcgtgctgt ttggcaaaca gcaactcggc ttcagcgttg
    23881 tgcaatggga tggcaaacaa tatcaaaatc tggcgaagca acacgtcgcc cgacgcggcc
    23941 cgcgcattgg ttcttttctg ctacaaacgc tcaatgaaat gcaaatgcag tcgcaggagc
    24001 agcaaacggc cagacttatc gtcaccagcc tgcttagcca ctgccgcgat ttgctcggca
    24061 gccaaataca gaccgcctca cgcagccagg ctctattcga agctattcgt gattatatcg
    24121 acgaacgcta tgcctccgca cttacccgcg aatctgtcgc acaggcgttc tatatttcgc
    24181 caaattacct ctcgcacctg tttcaaaaaa cgggggccat tggttttaac gaatacctga
    24241 atcacacgcg actggagcac gctaagacat tattgaaagg ctatgacctg aaggtaaaag
    24301 aagtggcgca cgcctgcggc tttgtcgaca gcaactactt ctgccgtctg tttcgtaaaa
    24361 ataccgaacg ctcgccgtcg gagtatcgtc ggcagtacca cagccaactg acagaaaaac
    24421 cgactactcc agaataagat gggtttgctg cggcgcagaa agcactttgc gcactgcgct
    24481 catcaccctc tgcggctcac gcaggaaagc gtagatgctg cattgcacgt agcgacaatg
    24541 ttcaaatcgc tcagcaccgg caagctcgat atccgtaatt aacagcgcaa catccgcccg
    24601 gcggatatcc tcgtccgcta aacgattctc cgttcccaac gcgccctgag tttcaatgct
    24661 gacgccccac ttctctaact ggcacaactt ttccagccgt tccgccgcca tataagtatg
    24721 cgccacgccg cttacgcagg cggttactgc caccagatat gccattttaa cctcccacgg
    24781 taacctgaaa tccggcccgt tctgccattt cacgcatcgt tgccacatcg gctgacgacg
    24841 gcgcagcaac ctctttcatc gaccatgttt tccccagcag gcggtatttc ggttcgccgt
    24901 actgatgaaa cggtaacaga tggatctgcc tgatattcag cgggatcagc acgtccagcg
    24961 cctgctgcat attctcccgg ctgagcgtga aaccagggat caacggtaaa cgcgggatca
    25021 cgttgacacc ctcactcacc agcaaacgca gattctccag cacgcgttgc aggttcatct
    25081 tcaccacatc ccgagcctga gccgcatcca taatttttaa atcgaacaac acttcatcgc
    25141 acaatttcgc cagcggtaac agcttgctgg ctggcgcgtc tccggcagtt tcgatggcgc
    25201 atgacacacc ccatagccgc agtcgctgta aaaaacgggt cgcaaactcc gcctgcatta
    25261 acacttcgcc gccagaaagc gtgacgccac cgccggacgt gcgaaaaaaa atgtcatctt
    25321 tcatcacttc ccgttccagc gcgtcaaggc tgatatcgcg gccaatccgt tcaaatgccc
    25381 cggaggggca ttcatccgca tcacgcaaac atttcgcgca gtgcagacat ttcgcctctc
    25441 tgcgcaccgt ctggattttg ccggagatcg actccggatt agcacaccat gggcaaagat
    25501 gcggacagcc tttaaaaaag accaccgtac gaatgccctc gccgtcattc agtgaataac
    25561 gctgaatgtt gaaaatgcgc gccacatcat cgcggcgcat ttccacaacg ttacagctga
    25621 tgcgctgtcc ggcggatgat gtcatcctgg atctccttcg acaattcgac aaagaaggcg
    25681 ctgtatccgg caacgcgcac caccagcccg gcataatctt gtgggcgctg ttgcgcttcc
    25741 cgtaacgtgt cggcgttcac cacgttaaac tgaatatgct gcaacttaag ctgggtaaat
    25801 gcccgtaaga agtcagccag tttgcgtaat cccgcttcac cttccagggt cgccggagtg
    25861 aatttcacgt tcagcaacgt accgttagac agcagcgtat tatcgagctt actgactgat
    25921 ttcagcaccg ccgttggccc ttgtgcatcc tgacccagca tgggtgacaa gccaccgtct
    25981 gccagttgtt ccccggcaaa acgaccgtct ggcgtcgcgc caaccaccga tcccaacggg
    26041 acgtgagcag aaacggtata cgatcctggc gtgaagtagc cgccgcgcgg gttctggtat
    26101 ttttccactt ctttgcagta gtggcgtaac agttcggcgc taatgttatc cacctcgtcg
    26161 atatcgttac cgtatttctc gaagcggtta attaagcgag cgcggacttt ttcgccttct
    26221 ggcgtggcga agttggcttt taataccgac agcaattcgt caaaacttaa acgctgttga
    26281 tcaaaaatca tccctttcaa cgcatggaga gaatcgctca ggttggcgat accgatcccc
    26341 tgtacgccgg agaagttata acgcgcgccg ccatcggtaa tatcgcgacc tttttccaga
    26401 caatcgctga taaaagacga gagtagcggt acaggagccc agtcgcgatg gccgatatca
    26461 caaatattgc tgccctcaac catcagggtg atgtagtggc tgatcttggc gcggatctgt
    26521 tccagtaaac cttcataagt cagcgcagcg ttgccttcat tttcatgcag gcagatttcc
    26581 atcactttca gcaggttaaa catcgcgatg tcatgcaagc cgtaggttct gccgggaata
    26641 gataattcca cacagcccac tacggaatag tcgcgcgcat cttccagcga aacgccacgg
    26701 ttgaggaacg ctggcaccac cacttcatcg ttaaagattt gcggaatacc ggtgccgagg
    26761 cgaatggttt ctgcggtttt catcaggaac ggcgtgtcga tcaaggcgtt agtgcgcacg
    26821 ccgaggttcg gttgcggtaa ttgcacgctt tgataagcgt caaggcaaag gaacgaaagc
    26881 acgttcaccg cgctacgtcc gttctcggtt aacccgccga gcagtgcggt atagccggta
    26941 gggaaacctg cgaaataacg cgcgctactg gtggagcgca acagcacgat gtcgttgcat
    27001 ttcacccata aagattcgag cagttctttc aggaatgccg gatcttcgcc ctgggttaat
    27061 gatgcctgat agaacggcaa catatactgg tcgaagcgcc ccaacgatag cgaactggcg
    27121 ttggattcgt attgcagaat gatgttcatg taccagaata actggcacgc ctgccagaac
    27181 gtctgcggct tatgttcggc gttatggcgg gagatctccg caatggtcag cagttcttcg
    27241 cgacgctggc catcagtgca gcttgccgcc atcgtttccg ccagttcggc gtaacgcaaa
    27301 atatgtatct gcgaggcttc cagcagtaac agcgctgcct gataaaagtg attctccggc
    27361 tgttgctgac aatgttgctg catctgtgcc accagctccc ccagcccgtg attcagcagg
    27421 cgcgggtaat caataataat gtgcccctgg cctttatccg tctggttgat gctgaaaatc
    27481 tgcgtgctgg tcgcggcttt tacttcatcc gtcatctgcc cgttgatgaa atctttcatc
    27541 gaacgttttt cccagtacgg gaacaactct tcgcggtaga tacgtttatc ttcttcgctg
    27601 atagcaaagc ggtcctgcgg acgcgtcggg aattgatcca gctctttcag cagccagtaa
    27661 gggtccattt ccggcgacat aatcccggcg cgcggtttta cggtgcggtt accggcaatc
    27721 agttcttcat cacgaatcga aatttcaaca tgttcaagga tatacgctgt tgctttcgcc
    27781 cgacgcatta tcaccggctc cccttcggtt tgccgatggc tggcggtata aagcaacgcc
    27841 cgctccagcg agatttcacg ggtattggca aacagtgcag ttttgaggcg agagatacga
    27901 ttcgtcatga ggaactcctg tgggaaagcg agcccgccct tgcgggcggg ataagaactt
    27961 aagcggtttg tgccaggtga gcttcaattt tgctcataat ggcgtcagca cgctttaccg
    28021 cgtcgctgat gttgacacgc acgatagtct tgccagcaaa acgctcttca aatttgatgc
    28081 cgatatcttt ggtcaaaatc accatatcgg cgcttgccac gtcttccgca gtcagttcat
    28141 tttccagacc aatcgacccc tgggtttcta ctttcacttc ccagcctttc gctttcgcgg
    28201 cgctttccag cgcctctgcc gccatataag tatgggcaac acctgaagga catgcggtta
    28261 ctgcgataat tttcgtcatg gctcgttcct caattaatta atttcaaaat ccagatccag
    28321 gtcgtcttcc ttctcttcag ttgaagaccc atttttacgc gccagacttt tcagcacgtt
    28381 cacgcagact gccgtcacaa ccgccccgac agccactgcc gcgatgtaac ccaacttacc
    28441 ttcaaccacc ggcagcacaa tcagaccacc ccagcctgca tagcactgcg caccgaccag
    28501 cgccgcagtt accgcaccac aaacagaacc gaccatgatg gaaggaatga cacgcaacgg
    28561 atcggctgcg gcgaaaggaa tcgccccttc agtaacgcca acacagccca tcaccagtgc
    28621 cgccttgccg gtttcgcgct cttctgcgga gaaattttta cgaccaatca gcgtcgccag
    28681 ccccatcccc agcggcggga tacaaatacc cacggcagca atggccacca cggtataaac
    28741 accctgagca acgcaaatca gcatgaaggc ataggccact ttgttaaccg ggccgcccat
    28801 atcgaacgcc agcatcagac ccatgatcac cgccagcata acaatgctgc cctgctgcat
    28861 cccctgaagc cactgggtca ggctgttggt caacgccccc accggctcgc ccagccccca
    28921 catcatgacg ccagcggtaa tcagtgtgcc gacgattgga atgatgaaga ttggcatcac
    28981 cgagcgcaga actttatgca ccggaatttt cttcaggtaa tgcaccacga tgccgccgat
    29041 aatcccggcg atcagcgcac caaagaagcc cgcgccaaag ctgttaccaa cccaggcacc
    29101 gatagcgcat ggagccagcg cagaacgttc tgcaatggaa taaccgatgt aagcggcgag
    29161 gaaaggcacc atcagcgtca aacctgcaac gccgatatca aacagttttt tcagattcgg
    29221 atcggctacg gcatccggca ctgcgccttt gccatacaac atgacggaaa ccgccagcaa
    29281 aataccgccc gataccacga agggaatcat gtgtgaaacg cccgtcatta aatgctgacg
    29341 ggtatttttc aggatctgca ccaactcatt cataagctgc tcccgggctt gcgaaagccc
    29401 atgtccatat ttttgatgga agaaacaata ggcaatcgca ggcggagcaa acagtggata
    29461 aaaaggccat ttactggact tttgtaatat cagtacaaaa atgcgatccg cctcataact
    29521 tgcgataatc cggaagtacg ccgcaaaacg gcgtcttgtg agacgcgtcg caaattgacg
    29581 gttacattac atttgtccag cttttggcag atttgtcaca tggcaggccg ttgccgtgcg
    29641 ctcttatcct gtaaacagaa aaccgtaccg ggagaaaggc aatggccctg attgtggaat
    29701 ttatttgtga gctacctaac ggcgtgcatg cgcgtccggc aagccacgtt gaaacgctgt
    29761 gtaatacttt ttcatcacaa attgagtggc ataacctgcg cactgaccgc aagggcaacg
    29821 ccaaaagcgc ccttgcgctg attggcaccg atacgctggt gggcgataac tgccagttac
    29881 tgatttccgg ggccgacgaa caggaagcgc accagcgttt aagccaatgg ctgcgcgatg
    29941 aattccccca ctgcgacgca ccgctggcgg aagtaaaatc tgacgaactg gaaccactgc
    30001 cgatttcact gaccaatctg aatccgcaaa ttatccgtgc tcgcaccgtg tgcagcggta
    30061 gcgcaggcgg cattctgacg ccgatctctt ctttagatct caatgcgctg ggtaatcttc
    30121 ccgcagccaa aggcgttgac gccgaacaat ccgcactgga aaacggtctg acgctggtac
    30181 tgaaaaacat agagtttcgt ctgctggata gcgacggtgc taccagcgcg attctggaag
    30241 cgcaccgatc cctggctggc gatacttccc tgcgcgaaca tttactggca ggtgtcagcg
    30301 ccggattaag ctgcgccgaa gcgattgttg ccagtgcgaa tcacttttgc gaagagttcg
    30361 cccgttccag cagcagctac ctgcaagaac gtgccctgga cgtacgcgac gtctgcttcc
    30421 agttactcca gcaaatctac ggtgagcaac gcttcccggc accgggcaaa ctgacgcagc
    30481 ccgccatttg tatggctgat gaactgaccc ccagccagtt cctcgaactg gataaaaatc
    30541 atctcaaagg tctgttactg aaaagtggcg gcaccacatc acatacggtg atccttgccc
    30601 gttcgttcaa cattccgacg ctggtaggtg tggatattgc tgcccttact ccgtggcagc
    30661 atcagacgat ttatatcgac ggcaacgcag gggcgattgt ggttgagcca ggggaatccg
    30721 tggcccgtta ttatcagcaa gaagcccgcg tacaggacgc tctgcgtgag caacagcgtg
    30781 tctggctgac ccagcaagcg cgtaccgcag acggtatccg cattgaaatt gccgctaaca
    30841 tcgctcactc cgtggaagcg caggccgcat tcggcaatgg tgcggaaggc gttggtttgt
    30901 tccgcactga aatgctctat atggatcgca ccagtgcacc gggtgaaagc gagctgtaca
    30961 acattttttg tcaggcgctg gagtctgcca acggacgcag cattattgtg cgcactatgg
    31021 acattggcgg tgataaaccc gttgattatc tgaacattcc ggcagaagct aacccgttcc
    31081 tcggttatcg cgcggtgcgt atttatgaag aatacgcgtc gttgtttacc acacaactac
    31141 ggtcgatcct gcgcgcctct gctcacggca gcctgaaaat catgatcccg atgatctcct
    31201 caatggaaga gatcttatgg gtgaaagaaa aactggcgga agccaaacag caactgcgca
    31261 atgaacacat tccgtttgat gagaagattc cactcggcat tatgctggaa gtaccatcgg
    31321 tgatgttcat tatcgatcag tgttgtgaag agattgattt cttcagtatc ggcagtaatg
    31381 acctgacgca atatctactg gcagtggatc gcgataacgc taaggtgact cgtcactaca
    31441 acagcctgaa tccggccttc ttgcgggcgc tcgattacgc cgtgcaggcg gtgcatcgcc
    31501 agggcaaatg gattggtctg tgcggtgaac tgggagcgaa aggttccgtg ctgccgttgc
    31561 tggtcggttt agggctggat gaactcagca tgagcgcacc atcaattccg gcggcgaaag
    31621 cgcggatggc gcaacttgat agccgtgagt gccgccagtt gctcaaccag gcaatggcct
    31681 gccgtacttc gctggaagtg gaacacctgc tggtgcaatt ccgcatgacc caacaggacg
    31741 caccgctggt caccgccgag tgcatcacac tggaaagcga ctggcgcagc aaagaagaag
    31801 tgctcaaagg catgaccgat aacctgctgc tggcgggccg ctgccgctat ccgcgtaaac
    31861 tggaagccga cttgtgggcg cgcgaggccg ttttctctac cggactgggc tttagtttcg
    31921 ccattcccca cagtaaatcg gaacacattg agcaatccac catcagcgta gcgcgtctgc
    31981 aagcgccagt gcgctggggc gatgatgaag cgcaattcat cattatgtta accctgaaca
    32041 aacacgccgc gggcgatcaa catatgcgca ttttctcgcg cctcgctcgt cgcatcatgc
    32101 acgaagaatt ccgtaatgcg ctggttaacg ccgcctctgc cgacgctatc gccagcctgc
    32161 tgcaacatga actggaactg taaaaggaaa catcatggaa ctgtatctgg acaccgctaa
    32221 cgtcgcagaa gtcgaacgtc tggcacgcat attcccgatt gccggggtga caactaaccc
    32281 gagcattatc gctgccagca aggagtccat ctgggaagtg ctgccgcgcc ttcaaaaagc
    32341 gatcggtgat gagggcattc tgtttgctca gaccatgagc cgcgacgcgc agggtatggt
    32401 gaaagaagcg aaacacctgc gcgacgctat tccgggcatt gtggttaaaa ttccggtaac
    32461 ctctgaaggt ctggctgcaa ttaaaatgct gaagaaagaa ggcattacta cgctgggaac
    32521 cgcagtgtac agcgccgcgc aaggattact ggcggcgctg gctggagcca aatacgttgc
    32581 tccatacgtt aaccgcgtag atgcccaggg cggtgacggc attcgtactg tacaggagct
    32641 gcaagcgctg ttggaaatgc atgcgccaga aagcatggtg ctggctgcca gctttaaaac
    32701 accacgtcag gcgctggatt gtttgctggc tggatgtgaa tccatcacac tgcccttaga
    32761 tgtagcgcaa caaatgctta acacccctgc ggtagagtca gctatagaga agttcgagca
    32821 cgactggaat gccgcatttg gcactactca tctctaaagg agtaattatg gaccgcatta
    32881 ttcaatcacc aggtaaatac atccagggcg cagatgtaat taatcgtctg ggcgagtacc
    32941 tgaagccact ggcggaacgc tggttagtgg tgggtgacaa atttgtttta ggttttgctc
    33001 aatccactgt cgagaaaagc tttaaagatg ctggactggt agtagaaatt gcgccgtttg
    33061 gcggtgaatg ttcgcaaaat gagatcgacc gtctgcgtgg catcgcggag actgcgcagt
    33121 gtggcgcaat tctcggtatc ggtggcggaa aaaccctcga tactgccaaa gcactggcac
    33181 atttcatggg tgttccggta gcgatcgcac cgactatcgc ttctaccgac gcaccgtgca
    33241 gcgcattgtc tgttatctac accgatgagg gggagtttga ccgctatctg ctgttgccaa
    33301 ataacccgaa tatggtcatt gtcgacacca aaatcgtcgc tggcgcacct gcacgtctgt
    33361 tagcggcggg tatcggcgat gcgctggcaa cctggtttga agcgcgtgcc tgttcccgta
    33421 gcggcgcgac caccatggcg ggtggcaagt gcactcaggc agcgctggca ctggctgaac
    33481 tgtgctacaa caccctgctg gaagaaggcg aaaaagcgat gcttgccgcc gaacagcatg
    33541 tagtgactcc ggcgctggag cgcgtgattg aagcgaacac ctatttgagt ggcgttggtt
    33601 ttgaaagcgg cgggctggcg gcggcacacg cggtacataa cggcctgacc gctatcccgg
    33661 acgcgcatca ctattatcat ggtgaaaaag tggcattcgg tacgctgacg cagctggttc
    33721 tggaaaacgc gccggtggag gaaatcgaaa ccgtagctgc gcttagccat gcggtaggtt
    33781 tgccaataac tctcgctcaa ctggatatta aagaagatgt cccggcgaaa atgcgaattg
    33841 tggcagaagc ggcatgtgca gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc
    33901 cagatcaggt ttacgccgca ctgctggtag ccgaccagta tggtcagcgt ttcctgcaag
    33961 agtgggaata acctactcca aactcccggc ttgtccggga gtttgaacgc aaaattgcct
    34021 gatgcgctac gcttatcagg cctacgcaat ctctgcaata tagtaaattt acgtactttt
    34081 gtaggccgga taaggcgttc acgccgcatc cggcacgctg tgccagtaat ctgaaacttc
    34141 caccttgttc gggtgttttt ttatccgctt ctggacattt tctccacaga acaatcgtta
    34201 gcccggaagg tcgaatctgt gactcttatt ttcacggacg aaacagatga aagcttcact
    34261 ggcgttattc agtcttctga cgttatttac cagttactcc ttaaaatctc ccgccgttcc
    34321 accaaccgtg gtacaaatcc aggctaacac caatcttgcc atcgcagacg gcgccagaca
    34381 gcaaatcggc agcacgctat tttacgatcc ggcgtatgtg cagcttacct atcctggcgg
    34441 tgatgtcccg caagaacgcg gcgtatgttc cgatgtggtg atccgtgcat tgcgcagcca
    34501 gaaggtcgat ttgcagaaac tggtgcacga agatatggcg aagaattttg ctgcgtaccc
    34561 gcaaaaatgg cagttaaagc gcccggacag caacatcgat caccgtcggg tgcctaatct
    34621 ggaaacctgg tttacccgcc acgataaaac acgccccacc agcaagaacc ccagtgacta
    34681 tcaagcgggc gatattgtct cctggcgatt agataacggg ctggcacata ttggggtggt
    34741 gtcagatggc ttcgcccgcg acggtacgcc gctggtgata cataacattg gcgcaggcgc
    34801 gcaggaagaa gatgtactgt ttagctggcg gatggtgggg cactatcggt attttgctaa
    34861 ataagagatc tccccacatg aagaaaaaat tcagatcctt tttacactga cggcttcttt
    34921 tttcttacgg ctcaccagcg ccagtgccag tacaatcagc acaataccgc taccttcaac
    34981 tgcccccgga ttctcaccga gcagccacca ggaaaagagt acgccgcaaa cgggaacggc
    35041 cagtgtgctt aagctggcaa tactggcagg caagtttttc aatacaaaca accataagct
    35101 ccacgccagc gctgtcgcca gaatcgcact gtaggccagc gcccagaaca cggtgggttg
    35161 ccagtcaatt tcacgttgcg gcaccagtaa agcgaccacg ctcatcacca gcgccgcata
    35221 cagcatctgc caggatgtta gcgacaataa atccacgcgc ggatggcggg catacagacg
    35281 tttagcaaca atcgcgctcg ccccccaact cacaccggag aggattgcca gcatggcact
    35341 tttcatcgaa gagaaatcga gttgccacgg ctgcaacacc aaaaataagc cgaaagcggc
    35401 aatcagtatc gcgaaatatt gcccacgtcg caggcgttca ccgagaaaca acgcggcgaa
    35461 aatcaccacc cagaacggca tggtatagct caggattgcc accttccccg ctccgccgct
    35521 gaccagcgcc cactgtgcca ggccaaccat cccgcaggtt tgtaacaggg caatggctaa
    35581 ggtgtattta aacggcgtcg ggcgcattcc gcgaccacgt aataaaagaa cgatgaataa
    35641 aacgagagcg ccgaaaatgc agcgtaaggc ggtgaagtcg aaggcaccga tgtagctggt
    35701 gacttgcttc atgaaaatcc agctataact ccagataagt gtgagcacaa ccaggccact
    35761 gattgccagt gggttgctct ttcctgcggc agacatgaga gatccagtgt gtagttttcc
    35821 tgtggacact atacgccggt aaagaaactt cagcgactgc atgaaaggca gtcgctgaaa
    35881 aaggtcagat tacagcaggt cgaaacggtc gaggttcatc actttcaccc acgctgccac
    35941 gaagtcttta acaaacttct cgtgggcatc gctgctggcg taaacttccg ccaccgcacg
    36001 caggacagag ttagaaccaa agaccagatc cgcacggctg gctgtgtatt tcacttcgcc
    36061 agtttcgcgg tcacggcctt cgaacagctc tttcgattcg tcggtcgctt tccactcata
    36121 acgcatatcc agcaagttca cgaagaagtc attgctcaat acgccaacgc ggtcagtgaa
    36181 gacgccgttt ttgctgccat cgaagttggc acccagtacg cgcatcccgc ccaccagcgc
    36241 agtcatttcc ggcgcggtca gcgtcagttg ctgggcttta tcaatcaaca gtgactcggt
    36301 ggtggaaacg tccagacgag cgcgatagtt acggaaaccg tcagcaattg gctccagcag
    36361 ttcgaacatc tcaatgtcag tctgatcctg acgcgcatca acgcggcccg gcgcaaacgg
    36421 tacatgaatg ctcaaacctg cggcgcttgc ggctttctca acgccaacca cacctgccag
    36481 cacgatgata tccgccagcg aagctttgcc agactctttc tgaattttct ccagaactgg
    36541 cagagcacga acggccgcgg cgttcacatc ccagtcacgc tgcggcatta atgccagacg
    36601 cgcaccgttg gcaccaccac gtttgtcgcc accacggaag gtagaagcag acgcccaggc
    36661 caccgatacc agctcactaa cagacagacc agaatccgca atcgcgaatt tcagatcgat
    36721 aatgtcctgc tcggtcgggt tgtagatcgg ctgcggcagc ggatcttgcc agatcagatc
    36781 ttctttcggt acttccggtc cgatgtaacg agatttcggc cccatatccc tgtgcgtcag
    36841 tttgaaccag gcacgagcaa aggcttcgtt gaacgcctgc ggatcgttga ggaaacgacg
    36901 agagatcttc tcgaactcag gatcaaaacg cagcgtcagg tcggtcacca gcatcgtcgg
    36961 tttacgtttc ttcgacggat caaacggatc cgggataatt tccggtgcgt ctaccgcttc
    37021 aaactggatt gcgccagccg ggctgcgggt ctgtacccac tcatacttga acaagttctc
    37081 gaagaaatag ttgctccact gggtcggcgt ctgggtccag accacttcca gaccagaggt
    37141 aatggcatct gcgccaacgc cgctgccgta agtgctcgcc cagcctaaac cttgttcttc
    37201 aatcggtgct gcttctggat caggacctac gtttgatgtc ggaccggcac cgtgggtttt
    37261 acccagcgta tgaccacccg caatcagcgc cacggtttct tcgtcattca tgcccatgtt
    37321 gccgaaggtc gcgcggatag ctgctgccgc cgaaagtggt tcgccgctgt gatccgggcc
    37381 ttccgggtta acgtaaatca ggcccatctc ggttgcaccc agcggtgctt tcgccagcgc
    37441 ttccggatga cggtgagtca gccaggcttt ttcatcaccc cagttaacat ccagatccgg
    37501 ttcccagacg tcttcacgac cggcaccaaa accgaaggta cggaagccgg agttttccag
    37561 cgccacgtta cccgcgagga taaacaggtc ggcccaggag attttctgac catatttctg
    37621 tttgattggc cacaacaggc gacgcgcttt atcgaggctt acgttatccg gccaggagtt
    37681 cagcggtgca aaacgttgct gaccacgacc cgcgccgccg cgaccgtcga ttgaacggta
    37741 agtacccgcg ccgtgccagg ccatacgaat aaacagaccg gcgtagctgc cccagtcggc
    37801 tggccaccac ggttgagatt ctgtcagcag ggctttcaga tcttttttca ggccgtagta
    37861 atctaatttg ctgaattctt tgcggtagtc aaagtcctca cccagcgggt tagaacgatt
    37921 agaatgttgg tttaacaggt caacacggag ttgatttggc caccagtcgc gcgtggttgt
    37981 gcccccacct gcgctctggt cgtgaccgcc ctgatggaac gggcatttgc cggtggctgt
    38041 cgtgttatgg atatcgtctg acgtgctcat caatgtgctc ccctctacag tgttaccgtt
    38101 acgatacaca gcgttagaga gaagttataa ttgaattaat ccacggatgc gatagttgag
    38161 atcttacata ttgttggtta aagagatgta gatcaaattg atcgtaatta agactttttg
    38221 attatgtccg atttaggata aatgctttat aaaaaagggt tttagatttg tatccttcaa
    38281 gtaatgaggg agaaaacaag gctgataacc ttattttccc gccctcattt cgaggcagca
    38341 ttttgtgctc tgtttaaaat ttgtgatcac tgtgtgattt tcacaaaagg cacactattt
    38401 ataaaccagg tcgaactccc agtgtatggc aaatcgcgta actcatttca gcgcggttaa
    38461 gcgtatagaa gtggaaatct ttcactcctt cacggcttaa aatcttcacc atatccatgg
    38521 caatattcgc gcccaccagc ttgcgggttt cagcatcgtc atccaggccg tcgaacattt
    38581 gtgccatcca cgccggaata cgcacgttgg tcatgtcggc aaatttcttc gcctgtttaa
    38641 agttcgaaac cggcaaaatt cccggaatga tttccacatc aatgcctgcc gagacgcagc
    38701 gatcgcgaaa acgcaggtag ctttcgacat cgaagaagaa ctgagtaatg gcgcggttgg
    38761 ctccggcatc cacttttcgt ctcaggttga gtaaatcggc ctgggcactt ttcgcttctg
    38821 gatgtacttc cggatacgcc gccacggaga tatcgaaatc tgccacttct tttaacagtg
    38881 tcaccaggtc agaagcatac atttctggct taccacttcc cggcggcagg tcgccacgca
    38941 gcgccacgat atggcgaata ccgttattcc agtagtcgcg tgcaatggtc cgcagctcgg
    39001 caggcgtcgc atcaatacag gtaagatgcg gtgccgcttc caggccggta cgatctttaa
    39061 tgcctttaat aatgctgtgc gtacggtcgc gctcgccgga gtttgcgcca taggtcaccg
    39121 atacaaactt cggtttcagg ctgctaaggc gatcgatgga gttccacagg gtctgctcca
    39181 tttcactggt acgcggcggg aaaaattcga acgaaacgtt aatctgcccc tggacttctg
    39241 ccagactctg attcagggca tcccgctggc tggcgtgaaa aaagctcata ccttacctca
    39301 tcaatcgctt gtcgttatat gttgtgttgt gcacatctat ccgtttagac gtccagatgt
    39361 aaagatgaag gaaaagccga agggcgtcaa ccgaaaaata accaatgatg gtgaggaaaa
    39421 atcagggaag atgaaaaaaa ctcatcttaa ttagaggcga taaaagattt ctctatagcg
    39481 caatccacct cgcacgtttt gactcatatt ctgacaacac agcgacacct gggctaattc
    39541 gaaaaacaac gtcctgccac cggatggtga tgttaaggca tctgattcgg cctgcgccct
    39601 cgcgtaggcc gaatacagga tctgcgcaat caacgggtaa caaacagttg agtcaattca
    39661 gtgcgcactt ttatttgata tccttcacgc gcataccgtt gagctgttca tccgtaatgg
    39721 tgttgcccgc tttgaagtaa tcaaccaccg cgtgataaac gtaataaccg cccgttgtgt
    39781 tacgcgcttt cccttcggta aaggcggtaa aaccatctcc accatcagca aggaaactct
    39841 gagtagcaat gtgataaacc gtcgcatctt caatgggttt gccattcaga gtcagtgtga
    39901 ttacccgctg accaaccggc ttgctgctgt cgtacttcat ttccaggcct ttcgatacct
    39961 gtaaaacgcc attgcttaaa cttgcgccgt gttccatcag actgcgtaat tgtttacccg
    40021 tgagttccat cgtcaccagt tcgttgggga acgggaacgt actgatgacg ccgcccatcg
    40081 taatcgcccc tgcggggatc tcattgcgga taccaccaga gtttgttaac gccagttggg
    40141 tatttttacc cgctgctgcc agtaaagcgt cagccgccag gttcccgagg gaagccgatt
    40201 caccataggc acgttttaat tcaaccggcg attgcgccac cgtttgttgc accacttcat
    40261 caagcttttt attccagccg tcgatcacct gtttcgtttg ctgatcgggc ttccactcgt
    40321 cagcgtaaat ggttttaagc tcgaagtttt tcaccgtaaa attatgcggc ttctctttgt
    40381 agtcgagaac cagtttgccc acatcaatcc cgccgctgtc agtggagagg atgagcgtat
    40441 tgccgacttt aatcggttcc ggcgtaccca catgtgcatg tccggtgatc aaaatatcca
    40501 gccctttcac ctgacttgcc gtctgaatat ctttatccaa cgcgcgacgc acatctgtgc
    40561 ctcccatact ggactggcgg gccggaacac cttcgtggat cagggcgacg gtcaaatcaa
    40621 ccttgccttt gggttcatcg atataacgtt gtagccattt aatttcatcg cgcgcttcaa
    40681 tacccactcg cgtggcagca gataccgtat cattgaaggc aaatactccg tgcaaaccta
    40741 taacgccaat tttcacgccg tctttttcga tgatggtat
//
LOCUS       DAZFFE010000028        39902 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFFE010000028 DAZFFE010000000
VERSION     DAZFFE010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.028"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            132..3890
                     /gene="yfaL"
                     /locus_tag="ACHJ69_001727"
     CDS             132..3890
                     /gene="yfaL"
                     /locus_tag="ACHJ69_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO5014729.1"
                     /translation="MRIISLRKEYLSLLPSMIASLFSANGAAAVIDSCQGYDIKASCQ
                     ASRQSLSGNIQDWSIADGQWLIFSGMVNNASGGAVFLQHSAEFTISPQNETGMTLFAD
                     NSVSGEYNNGGAIFAKENSTINLANVIFDSNVAGGYGGAIYSAGTNDTGAADLRVTNA
                     VFHNNIANDGKGGAIYTINNDVYLSDDAFNNNQAYTSTSYSDGDGGAIDVTDNSTDNT
                     HLSGKTIINNTSFTNNYAEGYGGAIYTSSTTSPYLIDISVDDNYDQNNGVMIDENNSA
                     SGYDHSATAAAGGFMYIGHSVAEFNIAADKTLVIGNTSNDGAIDSLAGTGVIVKEGAG
                     ELVLNADNNAFTGEISIQNGEVTLGRSDELMNVGDTHCQSDPQDCFGLMVGSTVHSEY
                     QAELNVGNTQQTFVHSLTGFANGILNIDAGGNVTVNQGGFSGSIQGEGQLTVAQDGSY
                     LLTGAQSMALTGDIVVEDNAVLSLAGNQADLRAMQSDPQSIVLNGGVLDLSDFTTWDG
                     DSSYNDGLQISGSGGTVIGSNDVVDISSGDDLHIGGSDASQNGVYVVINAGDQRVTLA
                     NNNGYLGNTQIASGTLEVSDNSQLGNTSYNRSVIFTDPQQHSEMDVTTDVDTRSATTG
                     QGRNIEMRADGEIHVEDGVDTQWGGLMADSTGQQLDSVSTLTKSGGGTLELTASGTAT
                     SAVRVEDGTLKGEAENIIPYVSSLWVGEDGVFETGQNQDIRSIDATSGGDIDITDGTV
                     LRLTQQDTNQALDASLFSGDGTLVNATDGVTLAGELNTNLETDSLTYLSDVTVNGNLT
                     NTSGAISLQNSVAGDTLTVNGDYTGGGTLFLDSELNGDDSASDQLVLNGNTSGNTTVV
                     VNPITGIGEPTSTGIKVVDFAADPAQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWY
                     LRSQEVNPTPPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYFNNLRAANQ
                     AFVMERRDHAGGDGQTLNLRVIGGRYHYTAVGQLAQHEDTSTVQLSGNLFSGHWGDDG
                     EWMLGIVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQRAWLDSWLQ
                     YVWFNNDVSEQDDGVDHYHSSGIIASLEAGYQWLPGHGVVIEPQTQVIYQGVQQDDFT
                     AANHARVSQSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDD
                     AVKQRGEIKVGITGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(4030..4752)
                     /gene="ubiG"
                     /locus_tag="ACHJ69_001728"
     CDS             complement(4030..4752)
                     /gene="ubiG"
                     /locus_tag="ACHJ69_001728"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO5014730.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            4899..7526
                     /gene="gyrA"
                     /locus_tag="ACHJ69_001729"
     CDS             4899..7526
                     /gene="gyrA"
                     /locus_tag="ACHJ69_001729"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO5014731.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTSEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7675..9363
                     /gene="yfaA"
                     /locus_tag="ACHJ69_001730"
     CDS             7675..9363
                     /gene="yfaA"
                     /locus_tag="ACHJ69_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO5014732.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPKKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNVLMFATYQDKILAFSSSDMLFK
                     DDQQDTEATAIASDLLSGKKRWETRFGLEARTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGGAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQALNKTRPARMDVIPTDGIVPLYINPQGMAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9360..9983
                     /gene="yfaT"
                     /locus_tag="ACHJ69_001731"
     CDS             9360..9983
                     /gene="yfaT"
                     /locus_tag="ACHJ69_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFO5014733.1"
                     /translation="MRHGLLVLICWLYCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANEALKVHDSKWLKSNGLSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYIIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            10007..14520
                     /locus_tag="ACHJ69_001732"
                     /pseudo
     CDS             10007..14520
                     /locus_tag="ACHJ69_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            14521..16170
                     /gene="yfaQ"
                     /locus_tag="ACHJ69_001733"
     CDS             14521..16170
                     /gene="yfaQ"
                     /locus_tag="ACHJ69_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO5014734.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TKVSALPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWATGSGTSKTVLTRYATALNRVLPVPTQVASGQCVLVDLFARYPLKKVTEEKST
                     TAFKPGVLNGRYRVTFANGNHMTFVSHGETTLLTVKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTYLQQNADRDGDCLSIPDSSATQRVSASPATVGARTMTAWT
                     QDLIYAGDPVHYHGSRVTEGTLSWRHATAQAGQGERYDQILAFAYPDNNLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16175..16951
                     /gene="yfaP"
                     /locus_tag="ACHJ69_001734"
     CDS             16175..16951
                     /gene="yfaP"
                     /locus_tag="ACHJ69_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO5014735.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQTINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPEGQSRQKMQFYSTPGAGAIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(17025..18209)
                     /gene="atoB"
                     /locus_tag="ACHJ69_001735"
     CDS             complement(17025..18209)
                     /gene="atoB"
                     /locus_tag="ACHJ69_001735"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFO5014736.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNIVTRKKTLVFSQDE
                     FPKVDSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMDESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(18240..19562)
                     /gene="atoE"
                     /locus_tag="ACHJ69_001736"
     CDS             complement(18240..19562)
                     /gene="atoE"
                     /locus_tag="ACHJ69_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFO5014737.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SIVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVMPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(19559..20209)
                     /gene="atoA"
                     /locus_tag="ACHJ69_001737"
     CDS             complement(19559..20209)
                     /gene="atoA"
                     /locus_tag="ACHJ69_001737"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFO5014738.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(20209..20871)
                     /gene="atoD"
                     /locus_tag="ACHJ69_001738"
     CDS             complement(20209..20871)
                     /gene="atoD"
                     /locus_tag="ACHJ69_001738"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFO5014739.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYHLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(21067..22452)
                     /gene="atoC"
                     /locus_tag="ACHJ69_001739"
     CDS             complement(21067..22452)
                     /gene="atoC"
                     /locus_tag="ACHJ69_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFO5014740.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(22449..24275)
                     /gene="atoS"
                     /locus_tag="ACHJ69_001740"
     CDS             complement(22449..24275)
                     /gene="atoS"
                     /locus_tag="ACHJ69_001740"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFO5014741.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGNRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILHQQTRDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISKLDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGSGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            24442..27291
                     /gene="rcsC"
                     /locus_tag="ACHJ69_001741"
     CDS             24442..27291
                     /gene="rcsC"
                     /locus_tag="ACHJ69_001741"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO5014742.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQTDVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLNKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(27491..28141)
                     /gene="rcsB"
                     /locus_tag="ACHJ69_001742"
     CDS             complement(27491..28141)
                     /gene="rcsB"
                     /locus_tag="ACHJ69_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO5014743.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(28158..30830)
                     /gene="rcsD"
                     /locus_tag="ACHJ69_001743"
     CDS             complement(28158..30830)
                     /gene="rcsD"
                     /locus_tag="ACHJ69_001743"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO5014744.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNENEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPEGKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETELFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            31569..32696
                     /gene="ompC"
                     /locus_tag="ACHJ69_001744"
     CDS             31569..32696
                     /gene="ompC"
                     /locus_tag="ACHJ69_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO5014745.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNAPESENNSWTRVAFAGLK
                     FQDIGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGQNGSVSGENDPDFTGHGITNNGRKALRQNGDGVGGSITYDYEGFGV
                     GAAVSSSKRTWDQNNTGLIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLG
                     WANKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGVVAGRNYDDEDILKYVDVGATYY
                     FNKNMSTYVDYKINLLDDNQFTRAAGINTDDIVALGLVYQF"
     gene            32808..33863
                     /gene="apbE"
                     /locus_tag="ACHJ69_001745"
     CDS             32808..33863
                     /gene="apbE"
                     /locus_tag="ACHJ69_001745"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO5014746.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKNLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            33937..35001
                     /gene="ada"
                     /locus_tag="ACHJ69_001746"
     CDS             33937..35001
                     /gene="ada"
                     /locus_tag="ACHJ69_001746"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO5014747.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYADASEALAAGFRPCKRCQPDKANPQQHRLDKITHACRLLEQETPVTL
                     ESLAEQLAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTQAILSAGFP
                     DGSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGSLSGY
                     RWGVSRKAQLLRREVENEER"
     gene            35001..35651
                     /gene="alkB"
                     /locus_tag="ACHJ69_001747"
     CDS             35001..35651
                     /gene="alkB"
                     /locus_tag="ACHJ69_001747"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO5014748.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGRLGWTTHRQGYLYLPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            35727..37370
                     /gene="yojI"
                     /locus_tag="ACHJ69_001748"
     CDS             35727..37370
                     /gene="yojI"
                     /locus_tag="ACHJ69_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO5014749.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSGSLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFILVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            37588..39234
                     /gene="mqo"
                     /locus_tag="ACHJ69_001749"
     CDS             37588..39234
                     /gene="mqo"
                     /locus_tag="ACHJ69_001749"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO5014750.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNSWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(39384..39893)
                     /gene="eco"
                     /locus_tag="ACHJ69_001750"
     CDS             complement(39384..39893)
                     /gene="eco"
                     /locus_tag="ACHJ69_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO5014751.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
BASE COUNT         9761 a         9779 c        10717 g         9645 t
ORIGIN      
        1 atctaaatta attatcagat aataacaaaa tagttattac atcaggggca gaacttacaa
       61 aagaaaaata ccctatatcg cataatgcga ttaaatacgc catcattata gaaaatgatg
      121 gttagatgaa tatgcggatt atctctctac gcaaggagta tttatcttta ctcccgtcaa
      181 tgattgcatc tcttttctct gctaacggtg ccgcagcggt cattgattca tgccagggat
      241 atgatatcaa agcgagctgt caggccagca ggcaaagcct ttcgggtaat atacaggact
      301 ggagcatcgc ggatggacaa tggttgattt tttcgggtat ggtcaataat gccagcggtg
      361 gcgcagtatt tttgcagcac agtgctgaat ttacgatatc accacaaaat gaaacaggga
      421 tgaccctgtt tgctgataac tcggttagtg gcgaatataa taatggtggg gctatctttg
      481 ctaaagaaaa ctcaacgata aatcttgcga atgttatttt cgacagtaat gttgcaggag
      541 gctatggcgg agcaatctat tctgccggta ccaatgatac cggtgccgcc gatttacgtg
      601 tcactaacgc cgtatttcac aataacatcg ctaatgacgg caaaggcggt gcaatttata
      661 ccatcaataa tgacgtctat ttaagtgatg atgcttttaa taataaccag gcatatacat
      721 caacaagtta tagtgatggg gatggcggcg ctattgatgt tacggataat agtacggata
      781 acacacatct ttcaggaaaa acgataatta ataatacctc ctttaccaat aactatgcag
      841 aaggttatgg cggagcaatt tataccagca gcaccacatc cccgtatctt attgatattt
      901 ctgttgatga taattatgac caaaacaatg gtgtgatgat tgatgaaaat aacagcgcct
      961 cgggatatga ccattcggcg acggcagcgg cggggggctt tatgtatatc ggtcatagtg
     1021 tggctgaatt taacattgcc gccgataaaa cgctggtaat aggcaatacc agcaacgatg
     1081 gcgcgattga ttctcttgcc ggaacgggag tcatcgttaa agagggagcg ggagagttag
     1141 tcctcaatgc cgacaataat gcgtttaccg gcgagataag tatccagaat ggcgaggtga
     1201 cgttggggcg cagcgatgag ttaatgaatg tcggcgacac gcactgtcag agcgatccgc
     1261 aggattgctt tggtctgatg gtcggcagca ctgttcattc tgagtatcag gcagaactga
     1321 atgttggcaa tacacaacaa acgtttgtgc actcattaac cggttttgct aatggcattt
     1381 tgaatatcga cgcaggcggc aatgttaccg taaaccaggg cggtttttcc ggctcgattc
     1441 agggtgaagg acagttgacg gtagcgcaag atggcagcta tctcctgaca ggggcgcagt
     1501 cgatggcatt aaccggcgat attgtggtgg aagataacgc cgtattgtcg ctggcgggga
     1561 atcaggccga tttacgggca atgcagagcg atccgcaatc gatcgtgcta aatggcggag
     1621 tattggatct ctctgatttc accacctggg atggcgatag ctcatacaat gacggtctgc
     1681 aaatcagcgg cagcgggggg acagtgattg gcagtaatga tgtcgttgat atcagcagtg
     1741 gcgatgattt acatattggc ggcagtgatg ccagccagaa tggcgtttat gtcgtcatta
     1801 atgcggggga tcagcgcgta acgctggcga ataacaacgg ttatctcggt aatacgcaaa
     1861 tcgcctccgg tacgcttgaa gtcagcgata attcgcaatt ggggaacaca agttacaacc
     1921 gttcagtgat cttcacagat ccccaacaac acagtgaaat ggatgtgacg actgatgttg
     1981 acactcgctc ggctacaaca ggtcagggca ggaatattga aatgcgcgct gatggcgaaa
     2041 tacacgttga ggatggcgta gatacgcaat ggggcgggtt gatggcggat agtaccgggc
     2101 aacaactgga tagcgtaagc acgttgacta aaagtggcgg cggtacgctg gagttgaccg
     2161 ccagcggcac tgcgacgtct gcggtacgtg tggaggacgg aacgctaaaa ggtgaagcgg
     2221 agaatattat tccttatgtt tcttcactgt gggtggggga agacggtgtt tttgaaaccg
     2281 ggcaaaatca ggatattcgt tcaatcgatg ccacctctgg cggcgatatc gacataaccg
     2341 atggtactgt gttgcgattg acgcagcagg atacgaacca ggcgctggat gcctcgctgt
     2401 ttagcggcga cggtacgctg gtgaatgcca ccgatggcgt gacgctggca ggtgagctta
     2461 ataccaacct tgaaactgac agcctgactt atctttccga cgtgacggtt aatggcaatc
     2521 tgaccaatac gtccggtgcg attagcctgc aaaatagcgt cgctggcgac acgctgacgg
     2581 taaacggtga ttacaccggt ggcggtacgt tgtttctcga cagcgaatta aacggcgatg
     2641 actcggcaag cgaccaactg gtgttgaacg gcaatacctc gggtaacacg accgtggtgg
     2701 ttaaccccat tactggcatt ggtgaaccga catctacagg tattaaagta gttgatttcg
     2761 cagctgatcc cgcgcagttt caaaacaatg cgcagttcag tctggcagga agcggctacg
     2821 tcaatatggg ggcgtatgac tacacgctgg tggaagataa caacgattgg tatctgcgat
     2881 cacaagaagt caatccgacg ccgccgcctg atccagaccc gactccagat cctgatccca
     2941 cgccggatcc cgacccaacc cccgacccgg aacctacgcc tgcctaccag ccggtgttga
     3001 atgccaaagt tggtggatat tttaataacc tgcgggcggc aaatcaggca tttgtgatgg
     3061 agcgacgcga tcacgctggt ggcgatggtc agacgctgaa tttacgcgtt atcggcgggc
     3121 gttatcatta cacggcggta gggcagttgg ctcagcatga agacacttct acggtgcagc
     3181 ttagcggcaa cctgtttagc gggcactggg gtgatgatgg cgagtggatg cttgggattg
     3241 ttggtggcta cagcgataac cagggcgaca gccgctcgaa tatgaccgga actcgcgccg
     3301 ataaccagaa ccacggttat gccgttgggc tgacctcaag ctggtatcag cacggtaatc
     3361 agaagcagcg ggcctggttg gatagctggc tgcaatatgt gtggtttaac aatgatgttt
     3421 ccgaacaaga cgatggcgtg gatcattacc actcgtcggg gattatcgcc tcgctggaag
     3481 ccggttatca gtggttaccg gggcatggtg tggtgattga accgcagacg caggtgattt
     3541 atcagggcgt gcagcaggat gattttaccg ccgctaacca tgcgcgcgtg tcacaatcgc
     3601 agggtgatga tattcagacg cggctgggtt tacacagcga atggcgtact gctgttggtg
     3661 tcacgccaac gttagatctg aattattatc acgatcccca tgcgacggaa attgaagaag
     3721 atggcagcac tatcagtgac gatgcggtga agcaacgggg tgaaataaaa gtgggaataa
     3781 cgggcaatat cagtcagcga gtttcgctgc gcggcagcgt ggcgtggcag aaagggagtg
     3841 atgattttgc ccagacggca gggtttttgt cgatgacagt gaaatggtaa tcgctgtctg
     3901 accggcactg ttgccggatg cggcgtaaac gccttatctg gcctacgggg agcatttgta
     3961 ggcctgataa gacgcggcaa gcgtcgcatc aggcttggcg cggttgccgg atgaatccaa
     4021 acaacaacct cacttattct gcgtatgcag catatagttc acatccacgc cggggccgag
     4081 cttaaaggaa ttagttatag ggttgtaatg cagcccggtc atatgccgtt ctttaagact
     4141 ggtctgatcc acccagccca gcaattctgc cggtttaata aacttcttca catcatgtgt
     4201 acctttaggc accatgcgca aaatatattc agcaccaacc accgccatca gccatgactt
     4261 gccgttgcgg ttaagcgtcg agaaaaagac atcgccgccc ggtttcacca gttgcgcaca
     4321 ggctctgacc actgactgag gatcggggac gtgctccagc atctccatac aggtcaccac
     4381 gtcatactgt ccggcatgtt ttgccgcgtg ctcttccacg gtttcctgca cgtaatccac
     4441 ctgaatgccg ctttccagtg cgtgcagttt tgccacctgc aatggctcaa agcccatatc
     4501 cagaccggtc accgtcgcgc cctcccgcgc catactctcg gccagaatgc cgccgccaca
     4561 accgacatcg agcacctttt tgccaaataa accgccagca cgctcggcaa tatagcccag
     4621 acgcagcggg ttaatgcggt gcagcggttt gaactcacct tcaagatccc accagcggga
     4681 ggcgacggct tcaaatttag cgatctcttc gtggtctacg ttgtggttta ccggcgattt
     4741 ttcggcattc attggcactt ctactccgta attggcaaga caaacgagta tatcaggcat
     4801 tggatgtgaa taaagcgtat aggtttacct caaactgcgc ggctgtgtta taatttgcga
     4861 cctttgaatc cgggatacag tagagggata gcggttagat gagcgacctt gcgagagaaa
     4921 ttacaccggt caacattgag gaagagctga agagctccta tctggattat gcgatgtcgg
     4981 tcattgttgg ccgtgcgctg ccagatgtcc gagatggcct gaagccggta caccgtcgcg
     5041 tactttacgc catgaacgta ctaggcaatg actggaacaa agcctataaa aaatctgccc
     5101 gtgtcgttgg tgacgtaatc ggtaaatacc atccccatgg tgactcggcg gtttatgaca
     5161 cgatcgtccg tatggcgcag ccattctcgc tgcgttacat gctggtagac ggtcagggta
     5221 acttcggttc catcgacggc gactctgcgg cggcaatgcg ttatacggaa atccgtctgg
     5281 cgaaaattgc ccatgaactg atggctgatc tcgaaaaaga gacggtcgat ttcgttgata
     5341 actatgacgg tacggaaaaa attccggacg tcatgccaac caaaattcct aacctgctgg
     5401 tgaacggttc ttccggtatc gccgtaggta tggcaaccaa catcccgccg cacaacctga
     5461 cggaagtcat caacggttgt ctggcgtata tcgatgatga agacatcagc attgaagggc
     5521 tgatggaaca catcccgggg ccggacttcc cgacggcggc aatcattaac ggtcgtcgtg
     5581 gtattgaaga agcttaccgt accggtcgcg gcaaggtata tatccgcgcc cgtgccgaag
     5641 tagaagttga cgccaaaaca ggacgtgaaa ccattatcgt ccacgaaatt ccttatcagg
     5701 tgaacaaagc gcgcctgatc gagaagattg cggaactggt aaaagaaaaa cgcgtggaag
     5761 gcatcagcgc gctgcgtgac gagtctgaca aagacggtat gcgcatcgtg attgaagtga
     5821 aacgcgatgc ggtcggtgaa gttgtgctca acaacctcta ctcccagacc cagttgcagg
     5881 tttctttcgg tatcaacatg gtggcattgc accatggtca gccgaagatc atgaacctga
     5941 aagacatcat cgcggcgttt gttcgtcacc gccgtgaagt agtgacccgt cgtactattt
     6001 tcgaactgcg taaagctcgc gatcgtgctc atatccttga agcattagcc gtggcgctgg
     6061 cgaacatcga cccgatcatc gaactgatcc gtcatgcgcc gacgcctgca gaagcgaaaa
     6121 ccgcgctggt tgcaaatccg tggcagctgg gcaacgttgc tgcgatgctg gaacgtgcgg
     6181 gcgacgatgc tgcgcgtccg gaatggctgg agccagagtt cggcgtgcgt gatggtctgt
     6241 actacctgac cgaacagcaa gctcaggcga ttctggatct gcgtctgcag aaactgaccg
     6301 gccttgagca cgaaaaactg ctcgacgaat acaaagagct gctggatcag attgcggaac
     6361 tgttgcgtat tcttggtagc gccgatcgtc tgatggaagt gatccgtgaa gagctggagt
     6421 tggttcgtga acagttcggt gacaaacgtc gtaccgaaat caccgctaac agcgccgaca
     6481 tcaacctgga agatctgatc acccaggaag atgtggtggt cacgctttct caccagggtt
     6541 acgtgaagta tcagccgctt tctgaatacg aagcgcagcg tcgtggcggg aaaggtaaat
     6601 ctgccgcacg tattaaagaa gaagacttta tcgaccgact gctggtggcg aacactcacg
     6661 accatattct gtgcttctcc agccgtggcc gcgtctattc gatgaaggtc taccagttac
     6721 cagaagctac tcgtggcgca cgtggtcgtc cgatcgtcaa tctgctgccg ctggaacagg
     6781 acgaacgtat caccgcgatc ctgccggtga ccgagtttga agaaggcgtg aaagtcttca
     6841 tggcgaccgc taacggtact gtgaagaaaa ccgtcctcac cgagttcaac cgtctgcgta
     6901 ccgccggtaa agtggcgatc aaactggttg aaggcgatga gctgatcggc gttgacctga
     6961 ccagcggcga agacgaagta atgctgttct ccgctgaagg taaagtggtg cgctttaaag
     7021 agtcttctgt ccgtgcgatg ggctgcaaca ccaccggtgt tcgtggtatt cgtttaggcg
     7081 aaggcgataa agtcgtctct ctgatcgtgc ctcgtggcga tggcgcaatc ctcaccgcaa
     7141 cgcagaacgg ttatggtaaa cgtaccgcag tggcggaata cccaaccaag tcgcgtgcga
     7201 cgaaaggggt tatctccatc aaggttaccg aacgtaacgg tttagtggtt ggcgcagtgc
     7261 aggtagatga ctgcgaccag atcatgatga tcaccgatgc cggtacgctg gtacgtactc
     7321 gcgtttcgga aatcagcatc gtaggccgta acacccaggg cgtaatcctt atccgtactt
     7381 cggaagatga aaacgtggtg ggtctgcaac gtgttgctga accggttgac gaggaagatc
     7441 tggataccat cgacggcagt gccgcggaag gggacgatga aatcgctccg gaagtggacg
     7501 ttgacgacga gccagaagaa gaataatttc atcgcttcat gccaaaaggg agccatctcc
     7561 cttttttgaa ttgaaaagtc caggctgtaa agtctgggct tttgtcgtat tagggcgcgg
     7621 taaagtttgg ctgtgcccgt aaaaaatggc tggctataca caaggaatgt ggcaatgagt
     7681 ggtgaaaaaa aggcgaaagg ctggcggttt tatggccttg ttggttttgg ggcaatagcg
     7741 ctgctatccg ctggcgtatg ggcgttgcaa tatgctggca gtgggccaaa aaaaacgttg
     7801 tcgccactgg tggtgcacaa caatctgcaa atcgatctca atgagccgga cctctttctc
     7861 gacagcgact ctctgagcca gcttcccaaa gatctcctta ccattccgtt tctccacgat
     7921 gttctgagcg aagatttcgt tttctattat cagaatcatg ccgatcgtct gggcattgaa
     7981 ggcagcattc gccgcattgt ctatgaacac gatctcacgc tgaaagataa gctcttttcg
     8041 tcactcttag accagcccgc gcaggcggca ctgtggcatg ataaacaagg tcatctttca
     8101 cattatatgg tgcttatcca gcgcagtggt ttaagcaaac tgctggagcc attgttgttt
     8161 gccgctacca gcgacagcca gttaagcaag acggaaatca gtagcatcaa gataaatagt
     8221 gaaactattc ctgtttatca gttgcgctat aacggcaata acgtcctgat gtttgcgact
     8281 tatcaggaca aaatattggc gttttccagt tcggatatgc tgtttaaaga tgaccaacag
     8341 gataccgaag ctacggcgat cgcaagtgat ttgttgagtg gtaaaaaacg ctgggaaacc
     8401 cgctttggcc tggaagcgcg tactgccgaa aaaacgccag tacgccagcg catcgtagtc
     8461 agcgccaggt tgctggggtt tggctaccag cgattaatgc cttcttttgc tggcgtgcgc
     8521 ttcgaaatgg gtaacgacgg ctggcatagc tttgtggcgt taaatgatga atccgccagc
     8581 gtagatgcca gtttcgattt tacgccggta tggaacagta tgcctgccgg agccagcttc
     8641 tgtgtggcgg tgccgtattc acacggtatt gccgaagaga tgctttcgca catcagccag
     8701 gaaaacgaca agctgaatgg ggcgttagac ggtggcgccg ggctgtgctg gtatgaagac
     8761 tcaaaattac aaaccccgct atttgttggt cagtttgatg gcactgccga acaggcgcaa
     8821 ttgccaggga aactgtttac gcaaaatatt ggtgcgcacg aaagcaaagc gccagaaggt
     8881 gttttgccgg taagccagac tcagcagggc gaagcgcaaa tctggcgtcg cgaagtgagt
     8941 tcccgatacg gtcagtatcc gaaagcgcag gcggcgcaac ccgatcaatt aatgtcggat
     9001 tattttttcc gcgtgtcgct ggcgatgcaa aacaaaacgc tgcttttctc cctcgatgac
     9061 acgctggtta ataacgccct gcaagcactg aataaaaccc gcccggcaag gatggatgta
     9121 atacccactg atggcatcgt tccgctctat atcaatccac aaggaatggc gaaactgctg
     9181 cgtaacgaaa cgctgaccag tctgccgaag aatctcgaac cggtttttta taacgccgca
     9241 caaactttat taatgccgaa gctggacgct ttatctcaac aaccgcgtta tgtcatgaag
     9301 ctggcccaga tggaacccgg tgccgcctgg cagtggctac ccataacctg gcaaccgcta
     9361 tgagacacgg gctgctggtg ctgatttgct ggctgtattg tgttgttgcc catagtgaaa
     9421 tgctaaatgt cgaacaatcc gggctgtttc gcgcgtggtt tgtgcgcatt gcacaggaac
     9481 agctccgcca ggggccaagc ccacgctggt atcaacagga ttgcgcgggc ctggtgcgat
     9541 ttgcggcgaa cgaggcgctg aaagttcacg acagtaaatg gctaaaaagt aacggtttat
     9601 ctaaccagta tttgccgcca gagatgacgc taacacctga acagcgtcaa ctggcgcaaa
     9661 actggaatca ggggaatggg aaaaccggcc cctatgtgac cgcgattaat ttgattcagt
     9721 acaacagcca gtttattggc caggacataa accaggcact gcctggcgat atgatttttt
     9781 tcgatcaggg cgatgcccag cacttaatgg tctggatggg gcgttacatc atctaccaca
     9841 ccggaagcgc cacgaaaact gacaacggaa tgcgcgcagt cagtctgcaa caacttatga
     9901 catggaagga cacccgatgg atacccaacg attccaatcc caatttcatt ggcatttatc
     9961 gtttaaattt tctggcgcga tagcagcgtg cttgtccctg tctcttgtgg gaacagggct
    10021 tgctaatgct gatgattcgc ttccttccag taactatgtg ccgcccgccg ggggaacatt
    10081 ctttttgctc gctgacagca gttttagcag cagtgaagag gcgaaagtgc gactggaagc
    10141 gccggggcgt gattatcggc gctatcagat ggaagagtac ggcggcgtgg acgttcgcct
    10201 gtatcgtatt cctgacccga tggcattttt gcgccagcag aaaacctgca tcgcattgtg
    10261 gtgcaaccgc aatatctggg cgacggactg aacaatacgc tgacctggct gtgggataac
    10321 tggtacggca aatctcgccg cgtgatgcag cgtactttct cttctcagtc acggcagaat
    10381 gtgactcagg cgttacccga attacagctc ggtaatgcca ttattaaacc ttcccgttat
    10441 gtacagaaca accagttttc accgctgaaa aaatatccac tagtggaaca gttccgttac
    10501 ccactatggc aggctaaacc ggtcgagccg caacaagggg taaaactgga aggcgcatcc
    10561 agcaatttca tttcgccgca gccgggcaac atctatattc ctctcggcaa acaagagccg
    10621 ggactgtacc tcgtcgaggc gatggtgggt gggtatcggg cgacgacggt ggtgtttgtt
    10681 tccgataccg tggcgcttag caaagtgtca ggtaacgagc ttctggtgtg gaccgcgggg
    10741 aaaaaacagg gtgaagcgaa gcccggctca gagatcttgt ggactgacgg tcttggcgtg
    10801 atgacctgcg gtgtgaccga tggcagcggt accttgcagt tacaacatat atcgccagaa
    10861 cgttcatata ttctgggtaa ggatgctgaa ggcggcgttt ttgtctccga gaactttttc
    10921 tacgaaagcg aaatctacaa cacccgcttg tatatcttta ccgatcgccc gctatatcgc
    10981 gcaggcgatc gtgtcgatgt taaagtgatg ggccgcgagt tccacgatcc gttgcattca
    11041 tcccccatcg tcagcgcacc ggcgaagctt tcggtgctgg acgccaacgg cagtctgttg
    11101 caaaccgtca atgtcacgct ggatgcgcgt aatggcggac aggggagttt ccgcctgcca
    11161 gaaaatgccg tcgccggagg ttatgagtta cgtcttgctt accgcaaaca ggtctatagc
    11221 agcagttttc gcgtggcaaa ctacatcaaa ccccattttg agattggttt agctctcgac
    11281 aaaaaagagt tcaaaactgg cgaagcggtc agcggcaaac tgcaactgct ttacccggat
    11341 ggcgagccgg taaaagatgc ccgcgtgcag ttaagtttgc gcgctcagca attatcaatg
    11401 gttggtaacg atttgcgtta tgccggacgt ttcccggtgt tgctggaagg cagtgaaacg
    11461 gtttccgacg ataatggtca tgtggcgttg aatctccccg ccgccgataa accgagccgt
    11521 tatttgttaa ccgcctccgc cagtgacggc gcggcgtatc gagttaccac caccaaagag
    11581 atcctcattg aacgtggcct ggcgcattac tctttaagta ccgccgcaca atacagtaat
    11641 agcggcgagt cggttgtgtt ccgttatgcc gcgctggaat cttcaaaaca ggttcctgtt
    11701 acgtatgaat ggttgcgtct cgaagaccgc acgagtcaca gcggagagct acagtcaggc
    11761 ggcaaatcct ttaccgtcaa tttcgctaaa cctggcaact acaatctgac attacgcgat
    11821 aaagacggct taattctcgc cgggttaagc catgcagtca gcggtaaggg cagcacgtcg
    11881 catactggta cggtagatat cgtggcagat aaaacactgt atcagcctgg cgaaaccgcg
    11941 aagatgctga ttaccttccc ggagccaatt gacgaagcat tattgacgct ggaacgcgat
    12001 cgcgtggaac atcagtcgct gctatcgcat ccggcaaact ggctaacgct acaacgttta
    12061 aacgataccc agtatgaagc ccgcgtaccg gtgagcaatt cctttgcgcc taacatcact
    12121 ttttcggtgc tgtatacccg taacggtcag tacagttttc agaacgccgg gattaaagtt
    12181 gccgttcccc agctggatat ccgggtgaaa acggacaaaa cccattacca gcctggtgaa
    12241 ctggtcaatg tcgaattaac ctcgtcgctg aaaggtaaac ctgtttctgc gcagttaacg
    12301 gtaggcgtgg tcgatgaaat gatctacgcg ctgcaaccgg aaatcgcgcc gaatatcggc
    12361 aaatttttct atccgctggg gcgtaacaat gtgcgtacca gctccagtct gtcgtttatc
    12421 agctacgatc aggcgctctc cagcgagccg gttgcgcctg gcgcgactaa ccgcagcgag
    12481 cggcgagtaa aaatgctgga acgtccacgg cgtgaagagg tggataccgc ggcctggatg
    12541 ccgtcactca caaccgataa acaaggcaaa gcgtatttca cgttcctgat gcctgattcg
    12601 ttaacccgct ggcgcatcac cgcgcgtggg atgaacggcg acgggctggt cgggcagggg
    12661 cgtgcttatc tgcgttcgga aaaaagtctc tacatgaaat ggagtatgcc aacggtgtat
    12721 cgcatgggcg acaaaccggc ggcaggactg tttatcttca gtcagcagga taacgaaccg
    12781 gtggcgctgg tgactaaatt tgcaggtgct gagatgcgcc agacgctgac gctgcacaaa
    12841 ggggcgaatt atatttcgct gacgcagaat attcagcaat ctggcttgtt aagtgcagaa
    12901 ctgcaacaaa atgggcaagt gcaggacagc attagcacaa aactgtcttt tgtggacaac
    12961 agctggcccg ttgaacagca gaaaaatgtc atgctcggcg gtggcgagaa cgcgctgatg
    13021 ttgcccgagc aggcgagcaa tatccggctt caaagtagtg aaacgccgca ggagattttc
    13081 cgcaacaatc ttgatgcgtt agtcgatgaa ccgtggggtg gggtgatcaa caccggtagc
    13141 cgtctgatcc cgctcagtct cgcctggcgt tcgcttgccg atcatcaaag tgccgccgct
    13201 aacgacattc gtcagatgat tcaggataac cgtctgcggc tgatgcaact ggcagggccc
    13261 ggtgcgcgct ttacctggtg gggtgaagat ggcaatggtg acgccttcct cacggcatgg
    13321 gcatggtacg ccgactggca ggccagccag gcgctcggcg taacgcagca accggaatac
    13381 tggcagcata tgctcgacag ctacgcggag caggcggata acatgccgtt attgcatcgg
    13441 gcgctggtgc tggcgtgggc gcaggagatg aatctgccgt gcaaaacgtt gttgaaaggg
    13501 ttggatgaag ctatcgcccg gcgcggaact aaaactgaag atttctctga ggaagacacc
    13561 agcgatatca atgacagcat gatcctcgat acacctgaat ctccactggc agatgcggtg
    13621 gcaaacgtct taaccatgac gttgctgaaa aaagcgcagt tgaagtccac tgtgatgcca
    13681 caggttcagc aatatgcgtg ggataaagca gcaaacagca accaaccgct ggcgcacacg
    13741 gttgtgctgc tcaatagtgg aggcgacgct acccaggcgg ccgctatttt aagtggttta
    13801 accgctgagc aatccaccat tgagcgcgcg ttggccatga actggctggc gaaatatatg
    13861 gcgacaatgc ctccagttgt gttgcctgcg cctgcgggcg catgggccaa acataagtta
    13921 accggaggag gcgaatactg gcgttgggtt ggtcagggcg tgccggacat tctctctttt
    13981 ggtgatgaat tgtcgccgca aaatgtgcag gtccgctggc gtgaaccggc aaaaacggct
    14041 caacaaagta acattccggt gaccgttgaa cgccagttgt atcggcttat ccccggtgaa
    14101 gaagagatga gctttactct gcagccggtg accagcaatg agattgacag cgatgcgctg
    14161 tatctcgatg aaattacgct taccagcgag caggatgccg ttctgcgcta tggtcaggtg
    14221 gaagtaccgc tgccgccggg ggctgacgtt gagcggacaa cgtggggcat ttcggtcaat
    14281 aaacctaacg ctggaaaaca gcaggggcaa ttgctggaaa aagcgcgtaa tgaaatgggc
    14341 gaactggcct atatggtgcc ggtgaaagaa ctgacgggaa cggtcacttt ccgccatttg
    14401 ctgcgcttct cgcaaaaagg gcaattcgtt ctgcctcctg ctcgttatgt gcgttcctat
    14461 gcacctgcac agcaaagtgt tgcggcaggg agtgaatgga ccgggatgca ggtgaaataa
    14521 gtgaactggc gcagaatcgt ctggctattg gcgttggtta ctctgccaac gcttgcggag
    14581 gagacgccgt tacaactggc gctgcggggc gcgcaacacg atcaactgta tcagctgtcg
    14641 tcatcaggcg tgaccaaagt ctcggcatta cctgacacgc tcaccacgcc attagggagt
    14701 ttgtggaaac tgtatgttta cgcctggctg gaggataccc atcaaccgga gcagccctac
    14761 cagtgtcgcg gaaactcccc ggaagaggtt tattgttgtc aggctgggga gagcataacg
    14821 cgcgataccg cgctggtacg ctcctgtgga ctctattttg cgccgcagcg gttgcatatt
    14881 ggcgcagatg tgtgggggca atactggcaa cagcgtcagg cacctgcgtg gttagcgtct
    14941 cttactacgc tgaagccaga aacctcggta acggttaaat cgctgcttga ttctttagcg
    15001 acgttacccg cgcaaaacaa agctcaggaa gtcttactgg atgtggtgct ggacgaggcg
    15061 aaaatcggtg tcgcatccat gctcggtagt cgggtacggg tgaaaacctg gagctggttt
    15121 gccgatgata agcaggaaat ccgtcagggt ggttttgctg gctggttaac cgatggtaca
    15181 ccgctgtggg ctactggcag tggcaccagt aaaacggtac tgacccgtta tgcgacggcg
    15241 cttaatcggg ttttgccagt gccgacgcaa gttgccagtg ggcaatgtgt gctggtggat
    15301 ctgtttgctc gttatccgct gaagaaagtt actgaggaaa agagtacgac ggcattcaaa
    15361 ccaggcgtgc tcaatgggcg ctatcgtgtg acgtttgcca atggtaacca catgacattt
    15421 gtcagccacg gtgagacaac gttactgacg gtaaagggca aactgaaatt acagtcgcat
    15481 ctcgatcgtg aagagtacgt tgccagagtt ctggatcgcg aggcgaaaag tacgcctcct
    15541 gaggcagcga aagccatgac cgtggcgatt cgcacgtatt tacagcaaaa cgccgatcgc
    15601 gatggtgatt gcctgagcat cccggacagt agcgctaccc agcgcgtttc agcttcgcca
    15661 gccactgtag gcgcgcggac gatgaccgcc tggacgcagg atctcatcta tgcgggcgat
    15721 ccggttcatt atcatggcag ccgggttacc gaagggacgc tttcctggcg gcacgctact
    15781 gcccaggcgg ggcagggaga gcgttatgat cagatacttg cgtttgctta tcccgacaac
    15841 aaccttagcc gctggggagc gccacgctca acctgccagt tattacccaa agcgaaagcc
    15901 tggctggcga aaaaaatgcc gcagtggcgg cgtatattac aaggtgagac gggatacaac
    15961 gaaccagacg tgtttgcggt ctgtcgttta gtctcaggtt tcccctatac cgatcggcag
    16021 cagaaacggc tgtttattcg taatttcttt acgcttcagg atcggctgga tttaacccat
    16081 gagtatctgc acctggcctt tgatggttat cccaccgggc ttgatgagaa ctatatcgaa
    16141 acgctgaccc gacaattatt aatggactaa tgctatgcga aagatttttc ttccgttgtt
    16201 actggtggcg ctttcgcctg tcgctcacag tgaaggtgtg caggaagtcg agattgatgc
    16261 accgctctct ggctggcatc cggcagaggg tgaagatgcc agtttttcac aaacgatcaa
    16321 ttacccggcg tcgtccgtca acatggcaga tgatcaaaat atctcagcgc agatccgcgg
    16381 caaaataaag aattatgctg cggcgggtaa agttcagcag ggccggctgg tggtcaacgg
    16441 tgccagtatg ccgcagagaa ttgaatcaga tggttctttt gcacgccctt atattttcac
    16501 tgaaggcagc aacagcgtgc aggtcatcag cccagagggg caaagccgac aaaaaatgca
    16561 gttttactca acgccgggtg ctggggcgat tcgcgcacgt ttacggctgg ttctctcgtg
    16621 ggatacggac aataccgacc tcgatcttca tgtcgttacg cctgacggtg aacatgcctg
    16681 gtacggtaac accgtgctga aaaacagtgg tgcactggat atggatgtca cgacagggta
    16741 cggccctgag attttcgcca tgccagcgcc agttcacggc cgttatcagg tgtatatcaa
    16801 ctactatggc ggacgcagcg aaacggaatt aaccaccgcc caactgacgc tgatcaccga
    16861 tgaaggatcg gtcaatgaga aacaggaaac atttattgta ccgatgcgta atgctggcga
    16921 gctgacgctg gtgaaaagtt ttgactggtg attaatgggg ttaccttcgc cgtcgatgtt
    16981 caagaaaacg cccgataact ttcgctatcg ggcgttttta ttggttaatt caaccgctca
    17041 atcaccatcg caattccctg gccgccacca atgcacagtg ttgccagccc cagcgtttta
    17101 tcgcgtgcct gcattgcatg taatagtgtg accagaatac gagcaccact ggcaccgata
    17161 ggatgcccca gcgcgatggc cccgccgttg acattcactt tctcaggatc aaagcccagg
    17221 gttttcccaa cggcaaggaa ctgtgcagca aatgcttcat tagcctcaat gagatcaata
    17281 tccgccagtt gcagccccgc cagttgtaac gctttttgcg tggcaggtac tggccccata
    17341 cccatcaatg cggggggcac gccaccgctg gcataacttt taatgcgagc caggggggta
    17401 aggcctgctg ccagcgccgc agattcatcc ataatcacca gagcggcagc accgtcgtta
    17461 atgccagacg cgttcccggc ggtgactgtt cctgctttat cgaaggccgg gcgcaatgcg
    17521 cctaacgctt cagccgtaga atccactttc gggaattcgt cttgactgaa gacgagggtt
    17581 ttcttccggg tgacaatatt taccgggacg atttcggctg taaaagcacc ggactcaatt
    17641 gcggctgcag ctttacgctg tgaatgtagc gccagttcat cctgcatttc acgggtaatt
    17701 ccgtactctt tagccacgtt ttcggcggta atccccatat gataaccatg ggtggcgcac
    17761 atcaggccat cgcgcaggat tacgtcataa acctgtccgt ctccaagacg ataaccagag
    17821 cgtgcttttg catcgagtaa gtagggcgct aaactcatat tttccatacc ccccgccaca
    17881 atgctctgtg cctgacctgc ctgaatcgcc tgtgcagcaa gcgccacgct tttcagacct
    17941 gaaccgcaca ctttgttgac cgtgaatccg cacaccgttt cagccagtcc gctttttagc
    18001 agcgcctgac gtgccggatt ttgccccagt ccggcttgca acacgttacc cataatcact
    18061 tcatcaacgt gtagtgaatc gatttttgca cgttcaatgg cggctttaat tactgtcgcc
    18121 cctaggtcga tggcgctggt ggacgcgagt gaaccgttaa aactaccgat agcagtacgt
    18181 accgcactga cgatgacaca atttttcatt ttatattcct tctgtttgta gggttgccgt
    18241 cagaacagcg ttaaaccaat gacgaaaatg acgccggaga agagcagggc agtgatgcaa
    18301 tagcccatga tgtcgcggac accgagtccg gcgattgcca gtgctggcag cgcccagaac
    18361 ggttgtgcca tgttcatcca ttgctcgccg taggcgatcg ccattaccga tttaccgaga
    18421 tcagcgccca gcgcctgggc tgcgggcatc acgaacggcc cctgaataac ccagtgcccg
    18481 ccgccagacg gaacggcgaa gttaatcagt gcagaactaa aaaaggtcat gaccgggaag
    18541 gtgtctttgt tcgcaacatt gatgaagaat tcggtaatga gtccgcccag accggagtgc
    18601 tccatcatca gttggatccc cgcgtagaag gggaattgca ccagaatacc ggcggtactg
    18661 cgtgctgccg cgctgatagc acgcatataa gccattggcg ttttatgtag cagcagaccc
    18721 gcaatcataa acatcaggtt gacggtattg atggtgatgt tgaagccatg ttcgctgaag
    18781 tacatcgcca ggtaagcgat accgagtgcg ccgatgatca acgtcagaat gcggctttct
    18841 tccagtcgct cggacggtgg agcatctttc ggtagttgct tttgaaaatc agcctcttcc
    18901 atgagtagtt tcggatcgat actcaccacg tcagacggtt ttggcatcat catgcgggtg
    18961 ataaatggca tcaccacaat caacgccaca gtgatgaaaa tattaaaacc actgaacaga
    19021 gtatcgccca ccgggatcag cccggcgata tgctcaaccg ggttgcccgg tgttgcagcc
    19081 aacagaggca ttgagccaga gaaaccgcca ccccaggtga gaaaaccaat gtaggcgcag
    19141 gcaatgagca acggataatc agaaccgggt actcgccggg cgacttcacg ggcgaacatt
    19201 gcaccgacaa ccaaaccaaa tccccagttg atgacacaag caactgaacc gaagaaagta
    19261 accagcatga cgccctgtac gggcgtcttt gcggcagagg cggcagtacg cagcaaactt
    19321 ttcaccggag cggagctggc aagggcatga ccggtaacga tgataagcgc catctgcata
    19381 ccaaacgcca gcaagttcca gaaaccgtca ccccacattt tcactatgct gatcggcgtt
    19441 tgtggtgtta accaaagcgc gatcacgaat gttagcaatg tcagcaacat ggcaaagatc
    19501 agtggatcgg gaagccaccg gctgacaaaa cgcgtcataa aacgcgatat gcgaccaatc
    19561 ataaatcacc ccgttgcgta ttcagatcgg cagctacttc aaaccgagct tctgttttgg
    19621 cacgcacggt ggctaaatca cacccgtcgg caatttcggt gagccacatt ttgccgtcaa
    19681 taaaacgaaa gacagccagt tcagtaacca gcatatgcac cgcatgttgc gcagtgagtg
    19741 gcatggtgca gcggcgcaaa atttttgctg aaccatcttt ggcgcaatgt tccatggcga
    19801 tgatcacttt gcgcgacccg gtcaccagat ccatcgcgcc ccccataccg ggcaccattt
    19861 tcccaggcac tacccagttc gcgaggtttg cttcttcgtc tacttgcaaa ccgccgagca
    19921 cgcaggcatc aatatgaccg ccacggatta gcgcaaatga catggcgcta tcaaacatgg
    19981 ctgcaccggg taaaatgccg cacggttgtc cgccagcgtt caccagatcg ggatgcgctg
    20041 ttgtgaccgg gcctaaaccg aggaagccgt tttccgattg cagagtgata tgaataccct
    20101 ctggtaaata attggcgacc attgtgggta aaccgatccc taagttaacg atgtcaccat
    20161 cacgaagctc ttgcgccaca cggcgcgcaa tacgttgttt cgcatccatt atttgctctc
    20221 ctgtgaaacg atgatgtggt cgataacggc accaggggtg acaatatggt caggttgcag
    20281 ctcgccggtt tcgaccagtt catccggctc taccagcgtg atatcagccg caagggctat
    20341 cagggggtta aagttgcggg cgctaaggtg ataggtcagg ttgccaagtg tgtcgcaacg
    20401 atgagcgcga attagcgcca ggtcggcgcg cagcgggcgt tcgagcagcc aggttttacc
    20461 gtcgagtgtc agtgtctgtt tgccttcctc tacgacggtg ccgacaccag ttggggtgag
    20521 aaaaccacca agtccagctc cgccacagcg aatttgctcg attagcgtac cttgcggcac
    20581 cagaacgacg tccatctcac cagatatcat gcgccgacct gtttccgggt tggtgccgat
    20641 atgtgaagca atcactttgc ggactcgacc attgacgatg agcggaccga tgccggtatc
    20701 aacaaacgcg gtatcattgg ctatcaatgt caggtcgcga acaccagatt ccagtaatgc
    20761 ttcaaccagg cgggatggag tgccaatccc cataaatccg cccaccatga tggtcatgcc
    20821 gtcacgaaag aagccggtgg cgtcttgtaa tgtcatcaat tttgttttca ttttttatcc
    20881 ctcttgcata caccgttgtg ggtacagtca gggaatagca aggggagtgc cagaatgaga
    20941 ttattgaatg ttgttattta actaattgaa tagaaaagga taaattagat ggggattcat
    21001 aaggagaatg agcggctatg cagaaaattg cgcactgtgc aaatttctgc atagcaagtt
    21061 ttggcgttat acatccgccg gatcgatacc gtattcctgg agtttataca tcaatgcacg
    21121 gcgactgatg cccagcatta aagcagtgcg ggttcggttt ccttcttgtt gttccagcac
    21181 ttccataatg atgcgttttt cgacgcgttt aatttcctct tttaaattac gctcaccgac
    21241 aggggctgtt tttgcttcac cagcattaca gactggctga cgaatctgcg gcggaagatc
    21301 ctcagaaaaa atgatcgggc ctgaattcat cacgacggcg cgttcaataa cgttggaaag
    21361 ctctcgaata tttcctggcc atgaccaggc ggtaagcagt gacattgcca tcggatcgat
    21421 gtcgataata tcgcgctgat tctcactact gaatttttgc aaaaagtgat tagctaacag
    21481 ggaaatatct tcccggcgat cgcgcagagg cggcagtatt aaatgaataa cgttaaggcg
    21541 ataaaagaga tcttcacgga aggtgccttc tttcaccatt gcctgcaagt cgcggttggt
    21601 ggcagcaatg atgcggatat caacttttat ggtctgatgg ccgccaatcc gttcaaattc
    21661 ccgttcctgc agaatgcgta gtaatttggc ttgcagtacc agcggcattt cgccaatttc
    21721 gtcgaggagc aaagtacctt cgttggcacg ttcaaataat ccctgacgca aggtttgtgc
    21781 accagtaaat gcaccttttt catgaccaaa cagttcactt tcgagcaacg attccggcag
    21841 cgccgcgcag ttgactttaa tgaacggccc ctttgcccgc cgcgaattgt agtgaatcgc
    21901 tctggcaatc aactctttcc cggtgccgct ttcaccgcta atcaagacgc tggcctgaga
    21961 aagggcaatt ttggcggtgt ctttgcagat gtccatcatc gccgggctgt tggtgagaat
    22021 gtgcccccat tgccagctgg tgctcagtgc ctggtgcaga tgacggatct cttttttcat
    22081 tgactggagt tgtaaagcgc gctgaacgat taaattcaac tcatcgagat caaacggttt
    22141 gataacatag tcgaaggctc cgcagcgtag tgcttcgacg gcggtttcca cttcagcata
    22201 ggccgtcatc agaataacgg gtgtccgggt ctcatggctg cgcatctcct ttagtgcctt
    22261 gatgccgtcc atctctggca tgcggatatc catcaacacc acgtcagggt gaatatcggc
    22321 aaacaggtgt aatgctgtgc gcccgttgtt cgcacaatgt gtttcgaatc cttgtagtgc
    22381 aaaagcggtg ctcagcatac ggcgaacatt atcttcatca tccacaataa ggatgcgatt
    22441 aatagcagtc atacagtctg atttccctgc gggttgatcg gtaaaataag cgtgaaggtt
    22501 gcgccgtagc ccggcaaact ggcgacgcga atatcaccct gatgggcatt aatgatgcgt
    22561 tgacttaacg ccagaccaag cccggttcct gaggctttgg tagtgaaaaa gggatcgaag
    22621 atcttttttt gcagcgagag atcaatgcca ctgccgttgt cctctatcga aatggcctgt
    22681 tgcgagtcgc tatactgcca ggttcgaatg cgaattttcc ctcgtgcact gatagcctgg
    22741 acggcattga tcaggatatt cagtagtacc tgtttgagca gttcacgatc ggcgttaatc
    22801 gggcttaatt cattatccag tttgcttatg aagtcgaccc gcgcttgtac gccggcggtt
    22861 tgtaccagta ccagagtttc ttcaaccaat gcattgaggc tgacttgttg ccattgactg
    22921 tggcgtggac gtgaaaattc gagcaattgc tgaataactt tgttaattga atcgatttct
    22981 ttgagtacta cggacagata ttcctgatgt attgggtcac gggtttgttg gtgcaagatc
    23041 tgtacataac cacgaatagc tgttagcgga ttacgtactt catgcgccac gccagccatc
    23101 agctcaccca gtgtggcgag gcgttctgct tgcgccatgc ggcgctgggt ttctttgcgg
    23161 gcagttaaat cagagaaaat caccaaagca cctatcattt caccgtgcgt gttatgaata
    23221 cgactggtcg tgacactgag ttcaatggtg cggtcacggc ctggaaaact gatctccagt
    23281 gctacatgtt cggtgccatg ttccagcgta tccagtactg gactgtagaa ctgagtattg
    23341 tcgaacaaca tggagtaagg ctgccctacc agttcatgac gttgatagcc agtgatgact
    23401 tctgctgctg ggttcatggt ggttacatca ccctggcggt caatggcaat gacgccatcg
    23461 gcagcgtttt caataatcag atcgttaagt gttcgcgttt cacgcagtgc ctgggcgagg
    23521 ttgttaacac tctgactgat ttgccccatt tcaccgggca attgtggtaa tcgagtggga
    23581 atattttgtg ccagagtcga gaggccatcg gtgatgatat ctatattggc gctcaggcga
    23641 cgggagaaaa ggacaatcaa cagcaggctt atcagcaaac cggcggtgag cacaatgata
    23701 atcctcacat ccattttcca ggcctggcgg cgaatatctt cggttaattc attggcccag
    23761 atatagccga ggatttcacc attacgctca atgggaatca ttgaattcaa aatatcgccg
    23821 cgcacctgcc tgcctgaata aaccaaaggg gtatttgtac gcatgacttc gcgaccaggg
    23881 tgatctgcgg caatggtaac gcccacatta ttctgatata gcgctgaagg cgcgtaggtg
    23941 attatcgcat ccagcgtttt gttgtaataa ccagcgccga tgccagggaa ggcgtgagtg
    24001 atattttcgg taatgggggc aagttctgca tttaatgcgc ggatacgctc ctcacgcggt
    24061 aagtcgatgt agagatcgta gcgattgcct agcgcctgat taagcaggtt gaccacggca
    24121 gaaagttttt tctctttttc agataagact gctgaacgtc cttccgtttc tacgatataa
    24181 ccaatagtaa gcgttgggac aatgaccatc aggattgcca tcaggatcat ttgattgcgt
    24241 aagcggcgtg gataaatcca cttcatataa tgcatgctga aatccactag tcttgtccgg
    24301 tatatgacga ttatcagagg ttaaggtgat gatttctcgg cggtgtatca tattccagag
    24361 aagagagaac attgcggtaa cacgctttta ccgctacctt aaccacactc catcggtcac
    24421 ctgaggcgga gcttcgcccc tttgaaatac cttgcttctt ttcgtacaac cctgaaagcc
    24481 tcgcgctaca tgttcagagc attggcgtta gtgctctggc tgttgattgc tttttcatcc
    24541 gttttttaca tcgttaatgc gttacatcag cgagaatcgg aaattcgtca ggaatttaat
    24601 ctgagttccg atcaggctca gcgctttatt caacgcacct ctgatgtgat gaaagagctg
    24661 aagtacatag ctgaaaatcg cttatcggca gaaaacggtg tgctttcccc gcgtggacga
    24721 gaaacgcaga cggatgtgcc tgcgtttgaa ccgctatttg ctgactctga ttgttccgca
    24781 atgagtaaca cctggcgagg ttctctggag tcattggcgt ggtttatgcg ctactggcgc
    24841 gataattttt ctgcggctta cgatctaaac cgggtatttt taatcggcag cgataacctc
    24901 tgcatggcca atttcggtct gcgtgatatg ccagtggaac gcgataccgc gttgaaagcg
    24961 ttgcatgaac gcatcaataa atatcgaaat gcaccacaag atgatagcgg cagtaacctc
    25021 tactggatca gcgaaggtcc gcgccctggc gtcgggtatt tttacgcgtt gacgccagtt
    25081 tatctggcga accggttgca ggcgcttttg ggtgtcgagc aaaccatccg gatggagaac
    25141 tttttcttac ctggtacgtt gccgatgggg gttaccattc ttgatgaaaa tggtcatacc
    25201 ctgatttcgc ttaccggacc agaaagcaaa attaagggcg atcctcgctg gatgcaggaa
    25261 cgctcctggt ttggctatac ggaagggttc cgggaactgg tgctgaagaa aaatctgcca
    25321 ccctcatcgc taagcatcgt ttattcagtg ccagttgata aggtgctgga acgcattcgc
    25381 atgttgatcc ttaacgcaat tttgctgaat gtgcttgccg gagctgcatt gtttactctc
    25441 gcgcggatgt acgagcggcg tattttcatt ccggcggaaa gcgacgccct gcgactggaa
    25501 gaacatgagc aatttaatcg caagattgtc gcctccgcgc cagtgggtat ctgcattttg
    25561 cgtaccgctg atggcgtcaa tattttaagt aacgaactgg cgcataccta tctcaatatg
    25621 cttacgcatg aggaccgcca acggctgaca caaattatct gtgggcagca ggtcaatttt
    25681 gttgatgtcc tgaccagcaa caataccaat ctgcaaatca gcttcgtcca ttcgcgctat
    25741 cgtaatgaaa acgtggccat ttgtgtgctg gtggatgttt cttcgcgcgt gaagatggaa
    25801 gagtcgttgc aggagatggc acaagcagcg gaacaggcga gccagtcaaa atcgatgttc
    25861 ctggccaccg tcagtcatga gctgcgaacg ccgctgtatg gcattatcgg taacctggat
    25921 ctgttgcaaa ccaaagagtt accgaaaggc gtcgatcgtc tggtgacggc aatgaacaac
    25981 tcttccagcc tgttgttgaa aattatcagc gatattctcg atttctcgaa aattgaatca
    26041 gaacagttga agatcgaacc gcgtgagttt tcaccgcgtg aagtgatgaa ccacatcacc
    26101 gccaactatt taccgctggt ggtacgcaag cagttaggct tgtactgctt tattgaaccg
    26161 gatgtgccag tggccttaaa tggcgacccg atgcgtttac agcaggtcat ctccaacctg
    26221 ttgagtaacg ccataaaatt caccgatacc ggctgtatag ttttgcatgt tcgcgctgat
    26281 ggcgattatc tctctatccg tgttcgcgat accggcgtgg ggattccggc gaaagaagtc
    26341 gtgcgcttgt ttgatccctt cttccaggtc ggaacgggcg tacagcgaaa cttccagggg
    26401 accggtctgg gtctggcgat ttgtgaaaaa ctgatcagca tgatggacgg cgacatctcg
    26461 gtagattcag aaccgggaat gggcagccag tttaccgtgc gtattccgtt gtacggcgct
    26521 cagtatccgc agaaaaaagg cgtggaaggg ttgagtggta aacgctgctg gctggcggtt
    26581 cgcaatgcgt cgctctgtca gttcctggaa accagtttgc agcgcagcgg catcgtcgtt
    26641 acaacatacg aagggcagga accgactccc gaagatgtgt taatcactga cgaggtagtg
    26701 agtaaaaaat ggcagggcag agcggtagtg accttctgtc gtcgccatat tggtattccg
    26761 ctggagaaag cgccagggga gtgggtacac agtgtggctg ctccgcatga gctaccggca
    26821 ttgttggcgc gtatttattt gatcgagatg gagagcgacg atcctgctaa cgctctgccg
    26881 tcgacggaca aagcggtcag cgataatgac gatatgatga ttctggtcgt ggatgatcat
    26941 ccgattaacc ggcgtctgct ggcagatcag ttgggatcgt tgggctatca atgtaaaacc
    27001 gcgaatgatg gcgtcgatgc gcttaatgta cttaacaaga atcatattga tatcgtgctt
    27061 agcgacgtca atatgccaaa tatggatggt taccgcttga cgcaacgcat tcgtcagttg
    27121 ggactgacgt tgccggtaat cggagtaact gctaatgcgt tggctgaaga gaagcagcgg
    27181 tgtctggagt ccggtatgga cagttgcctg tcgaagccgg taacgctgga tgtgataaaa
    27241 cagacgctga cggtatatgc cgagagggtc aggaaatcgc gggaatcgta ggacggataa
    27301 ggcgtttacg ccgcatctgg cactcagtgc aaatgccaga tgcgacgctg acgcgtctta
    27361 tctggcctac tttaatgtaa tctgttgaaa taatgggaat cgtaggacgg ataaggcgtt
    27421 tacgccgcat ccggcaatcc gtgcaaatgc cagataagac actaacgcgt cttatctggc
    27481 ctacaggtga ttagtcttta tctgccggac ttaacgtcac tgaagagaga taattcagca
    27541 gggcaatatc gttctcgaca cccagcttca tcatcgcaga tttcttctgg ctactgatgg
    27601 ttttaatact gcggttcagc tttttagcga tctcggtcac caggaagcct tccgcaaaca
    27661 ggcgcagaac ttcactctct tttggcgaga gacgcttgtc accgtaacca ccagcactga
    27721 ttttttccaa caggcgagaa acgctttccg gggtaaattt tttccctttc tgcagcgcgg
    27781 cgagagcttt cggcagatcg gtcggtgcac cttgtttcag cacgatccct tcgatatcca
    27841 gatccaatac cgcactaaga atcgccgggt tgttgttcat ggtcagaaca atgatcgaca
    27901 ggcttgggaa atggcgcttg atgtacttga ttaaggtaat gccatcgccg tacttatcgc
    27961 caggcatgga gagatcggta atcaacacat gcgcatccag tttcggcagg ttgttgatca
    28021 gtgctgtaga gtcttcaaat tcgccgacaa cattcaccca ctcaatttgc tcaagtgatt
    28081 tgcgaatacc gaacaagact atcggatggt catcggcaat aattacgttc atattgttca
    28141 tgtaataggc taccttgcta cagcaagctc ttgacataac tgtcaatgtc gctgatgtat
    28201 ttttctattc ctggaacatc cttctcacga atcagatgtt ccagcgtttc acataactgc
    28261 ttgccgggta ccagatttag catggcaaat acgcctttaa gacgatgagc cgtttgggct
    28321 aacgcagcaa agtcactggt tgctgcttca gtatatagcc tcttaacatc atccggtact
    28381 gtgtctacaa agagcgcata atagccgctg gcatggagtt gcgcattttc atctccgccc
    28441 agaggcgatt ctgtcacctc ttcctgcgcc agttgcactt caattaattg taagaccgct
    28501 tcctgcatag cgttgctcat attgaaattg acgcacaatt gaccaggccc aatttcccgt
    28561 acgccagact catcatcgct taaaagcaag ccagaggcag taagattaga cggattatcc
    28621 gttaaaaaga tatcataatc ttgactaatt aatctttcat cgggtgtgat acaggttgca
    28681 ccccaatttt ctaactggcg agtgacaata ttccgaattt ctgccgaagt aacatccacc
    28741 attacgcaga catcatccag taatcgctct tcttcctctt caacttccgg gtcagctgcg
    28801 agcattttga tatgcacaga gtagcgtgta ccaagcccat cccgcgtttt gatgtttaaa
    28861 tgaccgccca gtttacgcgc cagttgatcg ctcagccaga atgccagcgg gtccgccttg
    28921 ccatagcgat cattttgggt ctggttgata aacgggaagt gcaaattatc catttcatga
    28981 atgcttacgc cttctccagt gtccagaatg cggaacgtca ggcggtcttc ggaggactca
    29041 tcctgatcaa cctcaagggt gatttttccc aattgcgttg aggtcacggc atattgcatc
    29101 agtagtagca aaatacgtcg taaggcatcg cgatcgccgc ggcgcatatc gtgtgctttc
    29161 agatgattgt taatcagcag ttgcagacct ttacgcttga tggcaggcaa cactgaaggc
    29221 actacttcat caattaaatc ctgcacggag aacagctccg tctcactttt ccagctatcg
    29281 tccgcaagca tgttcgctaa ctgtatttca tcgaccagcc gcaccagcac atctgcctga
    29341 ttcgccagtt gtttgccttc cggggcgttg agtttagccg cgctctccgc cagggactgt
    29401 gcgggttctt tcagcgcatc gccaatgttt ttcataaagg tcatccgccc ctgctggttt
    29461 ttctcataca gacgctgcgc ctgcttgagt ttcttgttta ccagcacttc gcgatcctga
    29521 tcgcgaataa tgaaaatttg tgtgcgcggc gcaacctggc tgcggaacat gcggatctca
    29581 tacagctcgt tattgatcgt cgcctgaata atcccctgat gctgttccgc catggtggtg
    29641 atgttttgca gattcaaatg cggcagcaaa tgatcggcaa ttttgttact tatgactgtg
    29701 cggttcgatt cctgatcgtg aaccagcagg ccgagcggca gcagtgagac tatctcttca
    29761 ttaattgccc gtaaaatgcg caattcgtta ttgaccgccg tgctggggac gctttctgta
    29821 ctgcggctgg agaaatggcg gaatgtggta tagccaaata acgccagcgc cagcaaaccg
    29881 atgttcagca gcagtggcag cagaatgttt tgcaacgtat ccagcagtaa ggtgccgtaa
    29941 ggaacctgcc agaccaggcg catatcggta gagttgagtg ccgaggagat ttcaatcttc
    30001 gtactgttaa agtggatact gacgctatcc gtcccttctt tctcattttc attgtttccc
    30061 gttgccgtcg cgtctggctc aaggcggaaa ctgtccagcg gcatacccgg tgggatcaaa
    30121 tcattaatcg gcagatcaaa agccacgacc gttgccagat gtcccggctg gttgaacgtg
    30181 gtacgcaagg taaagtaatg accgttctgc caggccaggc ggcgcatgtt agaaaaactt
    30241 tcgcgttcat cgagggcgtt ggcctgttgc aacatctctg cacgacgtga atcaacaatg
    30301 tcgctgacgg tcgattcttt aaatccggag gtgagatctt tgaggggcag ggttgagatc
    30361 agcaccagac tgttatcctg accattcagg taatacatcg accacggtac attttctgcg
    30421 ccccacaatg tgtccagata ggtggacatc cgttgagtca tctcaagcgt tgagttgtcg
    30481 tgagagccaa agattaaggc ttccgttttg cggcgtggtt tctccagata gtagacatcc
    30541 tgtttcagtc gcgtctcttg taaaccttcg ccggaggagg gggaggtcgt cgcggcaatg
    30601 ttgtcgtaga tctgccaggt cacgtaacgc caggtatcga cgcgcttttg gattgcgtgg
    30661 gtaatgtcga caatctggta acttttatct ttcagccagg cgttaacggc gctttgtacc
    30721 attacaccca tcgtcaccag taacacaatg atcaacagta aaaagaagcg ggtaatgctc
    30781 cccggtagga gtgaaaagcg ggtcgtggcc gttgtctctt tctgacgcat tcgtgtttat
    30841 gacctgttaa aacttcgcga agtgataggg cagtataaag ggtacagtgt gaaattccag
    30901 actcttacct gattgatgat tctctattct ccagtgatat ttatcatcct gcattggggg
    30961 aaatagatgt acaagcgcta ttttgctgta caaataacct acaaaaagcc taacgaaatt
    31021 aattgcaaat aaacgaagat tgctggaaat tatgctgatg ttaattattt gtgaaatagt
    31081 taacaagcgt tatagttttt ctgtggtagc acagaataat gaaaagtgtg taaagaaggg
    31141 taaaaaaaac cggatgcgag gcatccggtt gaaatagggg taaacagaca ttcagaaatg
    31201 aatgacggta ataaataaag ttaatgatga tagcaggaga tattctagtt gcgagtgaag
    31261 gttttgtttt gacattcagt gctgtcaaat acttaagaat aagttattga ttttaacctt
    31321 gaattaatat tgcttgatgt taggtgctta tttggtcatt ccgcaataat cttaaaaagt
    31381 tcccttgtat ttacattttg aaacatctat ggcgataaat gaaacatctt aaaagtttta
    31441 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggacttg ccgactgatt
    31501 aatgagggtt aatcagtatg cagtggcata aaaaagcaaa taaaggcata taacagaggg
    31561 ttaataacat gaaagttaaa gtactgtccc tcctggtccc agctctgctg gtagcaggcg
    31621 cagcaaacgc tgctgaagtt tacaataaag acggcaacaa attagatctg tacggtaaag
    31681 tagacggcct gcactatttc tctgacaaca agtcagaaga tggcgaccag acctacatgc
    31741 gtcttggttt caaaggcgaa actcaggtta ctgaccaact gactggttac ggccagtggg
    31801 aatatcagat ccagggcaac gctccggaaa gcgaaaacaa ctcctggacc cgtgtggcat
    31861 ttgctggtct gaaattccag gacatcggtt ctttcgacta cggtcgtaac tacggcgttg
    31921 tttacgacgt aacttcctgg accgacgttc tgccagaatt cggtggcgac acctacggtt
    31981 ctgacaactt catgcagcag cgtggtaacg gcttcgcgac ctaccgtaac accgacttct
    32041 tcggtctggt tgacggcctg aactttgctg ttcagtacca gggccagaat ggcagcgtaa
    32101 gcggtgaaaa tgatccggat ttcactggtc atggcatcac caacaatggt cgtaaagctc
    32161 tgcgtcagaa cggcgacggt gtcggcggat ctatcactta tgattacgaa ggcttcggcg
    32221 ttggtgctgc agtttccagc tccaaacgta cctgggatca gaacaacact ggcctgatcg
    32281 gtactggcga ccgtgctgaa acctacactg gtggtctgaa atacgacgct aacaacatct
    32341 acctggctgc tcagtacacc cagacctaca acgcaactcg cgtaggttcc ctgggttggg
    32401 cgaacaaagc acagaacttc gaagctgttg ctcagtacca gttcgacttt ggtctgcgtc
    32461 cgtctgtagc atacctgcag tctaaaggta aaaacctggg tgtcgttgct ggtcgtaact
    32521 acgacgacga agatatcctg aaatatgttg atgttggtgc tacctactac ttcaacaaaa
    32581 acatgtccac ctacgttgac tacaaaatca acctgctgga cgacaaccag ttcacccgcg
    32641 ctgctggcat caacactgat gacatcgtag ctctgggtct ggtttaccag ttctaatctc
    32701 gatttatatc gaacaaaggg cctgcgggcc cttttttcat tgttttcagc gtacaaacac
    32761 agttttttgg tgtactcttg cgaccgttcg catgaggata atcacgtatg gaaataagct
    32821 ttacccgcgt ggcactgctg gctgccgcgc tcttctttgt tggttgcgat caaaaaccac
    32881 aacccgccaa aacccacgct actgaagtta ccgttcttga aggcaaaacc atgggtacct
    32941 tctggcgtgc cagcatcccg ggcattgatg ccaaacgcag tgccgaactt aaagaaaaga
    33001 ttcagaccca gctggacgcc gacgatcagc tgctttcgac ctataaaaaa gattccgcgc
    33061 tgatgcgctt taacgactcg caaagtttgt cgccgtggcc ggtaagtgaa gcgatggccg
    33121 atatcgtcac cacctcgctg cgcattggcg caaggaccga tggtgcgatg gatataaccg
    33181 tcgggccgct ggtgaatctg tggggctttg gcccggaaca acagccggtt caaattccga
    33241 gccaggaaca gatcgatgcg atgaaagcca aaaccggctt acagcacctg acggtcatta
    33301 atcagtcgca tcagcaatat ctgcaaaaaa acctgccgga tttatatgtc gatctctcca
    33361 ctgtcggcga aggctatgcg gcggatcatc tggcacgctt gatggagcag gaagggattt
    33421 cccgctatct ggtgtcggtg ggcggcgcgc tgaacagccg tggcatgaac ggtgaaggcc
    33481 agccgtggcg ggtagcgatt caaaaaccaa ccgataaaga aaacgcggtt caggccgtgg
    33541 tggatatcaa cggtcatggc attagcacct ctggcagcta ccgtaactat tacgaactgg
    33601 acggcaaacg cctttcccat gttatcgatc cgcaaaccgg gcgtcccatc gaacacaatc
    33661 tggtatccgt gacggtgatt gccccgacgg cgctggaagc cgacgcctgg gatactggct
    33721 taatggtgct cgggccagag aaagccaaag aagtcgttcg ccgggaaggg ctggcggttt
    33781 atatgatcac caaagagggc gatagcttta aaacctggat gtcgccgcag tttaaaagct
    33841 tccttatcag cgaaaaaaat taaagcgcaa gattgttggt ttttgcgtga tggtgaccgg
    33901 gcagactaaa ggctatcctt aaccagggag ctgattatga aaaacgccac atgcttaact
    33961 gacgatcaac gctggcaatc tgtcttagcc cgcgacccga atgccgacgg cgaattcgtt
    34021 ttcgccgtgc gcaccacagg catcttttgc cgtccgtctt gccgcgccag acatgctttg
    34081 cgggaaaacg tttctttcta tgccgatgcc agtgaggcgc ttgccgctgg ctttcgcccc
    34141 tgcaaacgtt gtcagccaga caaagccaat ccccaacaac atcgccttga taaaatcacc
    34201 cacgcctgcc gactgctgga acaggaaacg cctgtaacgc tggaatcctt agccgaacag
    34261 ctggcgatga gtccgttcca tttgcataga ttgtttaaag cgactaccgg aatgacgccg
    34321 aaagcctggc agcaggcctg gcgtgctcgc cgattgcggg agtctctggc aaaaggggag
    34381 agcgtgactc aggcgattct cagtgccgga ttccccgacg gcagcagtta ctaccgtaaa
    34441 gccgatgaaa cgctgggcat gacggctaaa caattccgtc acggtggcga aaatctggcg
    34501 gtgcgttacg cgctggctga ttgtgagctg ggccgttgcc tggtggcaga aagcgagcgg
    34561 gggatttgcg cgatattgtt gggcgatgat gatgcgacat taatcagcga gttgcagcag
    34621 atgtttcccg ctgccgacaa cgcgcctgcc gatctgacgt ttcagcaaca tgtgcgtgaa
    34681 gtgatcgcca gcctcaatca acgcgatacg ccgctgacgt taccgctgga tattcgcggc
    34741 actgcttttc agcaacaagt ctggcaggca ctgcgcacga taccttgtgg tgaaaccgtc
    34801 agttatcagc aactggcgaa cgccatcggc aaaccgaaag cggtacgggc cgttgccagc
    34861 gcttgtgccg ccaacaagct cgctatcgtt ataccttgtc atcgggtggt ccgtggtgat
    34921 ggctcacttt ccggttaccg ctggggcgtg tcgcgcaaag cgcaactgct gcgccgcgaa
    34981 gttgaaaatg aggagaggta atgttggatc tgtttgccga tgctgaaccg tggcaagaac
    35041 cactggcggc tggagcggtg attttgcacc gttttgcttt taacgctgcg gagcaactga
    35101 tccgcgatat taatgacgtt gccagccagt cgccgtttcg ccagatggtc actccaggcg
    35161 gatataccat gtcggtggcg atgaccaact gcgggcgtct gggctggacc acccatcggc
    35221 agggttatct ctatttgccc attgatccgc aaacaaataa accgtggccc gccatgccgc
    35281 agagttttca taatttatgt caacgtgcgg ctacggcggc gggctatcca gatttccagc
    35341 cagatgcttg tcttatcaac cgctacgctc ctggcgcgaa actgtcgctg catcaggata
    35401 aagacgaacc ggatctgcgc gcgccgattg tttctgtttc tctgggctta cccgcgattt
    35461 ttcaatttgg cggcctgaaa cgaaatgatc cgctaaaaag attgttgctg gaacatggcg
    35521 atgtggtggt atggggcggt gaatcgcggc tgttttatca cggtattcaa ccgttgaaag
    35581 cggggtttca tccactcacc accgactgcc gctacaacct gacattccgt caggcaggta
    35641 aaaaagaata aaaataagaa ttattattgc tgtgcgcgcg aagatgttta aactgcgggc
    35701 tgtaattcat tgtccgggtt ttctgcatgg aacttcttgt acttgtctgg cggcagtatc
    35761 gctggccatt tatcagtgtg atggcgctaa gcctcgccag tgcggcatta ggcattggct
    35821 taattgcttt tatcaatcag cgccttatcg aaacggcgga taccagtctg ctggtgttgc
    35881 cggagtttct gggattattg ttgctgttga tggcagtcac tctcggatcg caactggcgc
    35941 tcaccacttt ggggcatcac ttcgtttacc gactgcgtag tgaatttatc aagcggattc
    36001 tggataccca cgtcgagcgc attgaacaac ttggtagcgg ttcgttgctg gcggggttaa
    36061 ccagcgatgt gcgcaatatc accattgctt ttgtgcgtct gccggaactg gtgcagggga
    36121 tcattctcac tatcggttca gcggcgtatt tgtggatgct gtcgggcaaa atgttgctgg
    36181 taacggctat ctggatggca gtcaccatct ggggtggttt tatactggtg gcgcgggtgt
    36241 acaaacatat ggcgaccctg cgtgaaaccg aagataagct gtacacggat tttcaaaccg
    36301 tactcgaagg gcgcaaagag ctgactctga accgggaacg cgccgagtat gtgtttaaca
    36361 acctctacat tcctgatgcg caagagtatc gccatcatat tatccgcgca gacaccttcc
    36421 atcttagtgc cgtgaactgg tcaaacatca tgatgctggg cgctatcggc ctggtgttct
    36481 ggatggcgaa cagtctcggt tgggctgata ccaacgttgc cgcgacctat tcgttgacgc
    36541 tgttattcct gcgtacgccg ctgctttcgg cggttggcgc attaccgacg ctgctgacgg
    36601 cgcaggtggc gtttaacaag ctgaacaaat tcgcgctcgc gcctttcaaa gcagagtttc
    36661 cgcgcccgca ggcttttccc aactggcaaa cgctggagct gcgtaacgtg acgtttgctt
    36721 atcaggataa cgcgttttcc gttgggccga ttaacctcac catcaaacgt ggtgaactgc
    36781 tgtttctgat tggtggcaac ggtagtggaa aatcgacgct ggcgatgttg ctgacgggct
    36841 tgtaccaacc acaaagcggc gaaatcttgc tggatggcaa acctgtcagc ggcgaacaac
    36901 cggaagatta tcgcaaactg ttttcggcag tgtttaccga tgtctggctg tttgatcaac
    36961 tgctggggcc ggaggggcaa cccgctaacc cgcaactggt tgagaagtgg ctggcgcagc
    37021 tgaaaatggc tcataagctt gagttaagca acgggcgtat tgttaacctg aagttatcaa
    37081 aagggcagaa aaaacgcgtg gcgctgttgc tggcgctggc agaagaacgc gatattatcc
    37141 tgctggatga atgggcggcg gatcaggatc cacacttccg tcgtgagttt tatcaggtgc
    37201 tgctgccgct gatgcaggag atgggtaaaa ctattttcgc catcagtcat gatgatcatt
    37261 actttatcca cgccgaccgc ctgctggaaa tgcgcaacgg gcaacttagc gagctgacag
    37321 gcgaagagcg cgatgctgct tcgcgtgatg ccgttgcccg gacggcataa tattacgacg
    37381 ctgacctggc ggactgctgc cggcaggtca atattctaaa aataccttcg tttctaataa
    37441 ccctataatt aatcaacgaa attataatgt ttctaaaatt agaatataat ttataaacat
    37501 tatttaaatg ttgttactta agtgttaccg ttgatgccgc gcaaatctca cctgcaataa
    37561 agcgactaaa agtaaggcat taacaagatg aaaaaagtga ctgccatgct cttctcgatg
    37621 gccgtggggc ttaatgccgt ttcgatggcg gcaaaagcga aagcgtccga ggagcaggaa
    37681 actgatgtac tgttgattgg cggcggcatt atgagcgcca cgttggggac ctatttacgc
    37741 gagctggagc ctgaatggtc gatgaccatg gtggagcgcc tggaaggtgt cgcgcaggag
    37801 agttcgaacg gctggaacaa cgccggaacc gggcattctg cactgatgga actcaattac
    37861 accccgcaaa atgccgacgg cagcatcagt attgaaaaag cagtcgccat taacgaagca
    37921 tttcagattt cccgccagtt ctgggcgcac caggtcgagc gcggtgtgct gcgtactccg
    37981 cgttcattta tcaataccgt tccgcatatg agctttgtct ggggcgagga taacgttaat
    38041 ttcctgcgcg cccgttacgc cgcgttgcaa caaagctcgc tgtttcgcgg tatgcgttac
    38101 tctgaagatc acgcgcagat caaagagtgg gcaccgttag tgatggaagg gcgcgatccg
    38161 caacagaaag tagcggcaac acgcacagaa attggcactg atgtgaacta tggcgagatc
    38221 acccgccagt tgatcgcctc cttacagaag aaatctaact tctcgctgca actcagcagc
    38281 gaagtccgcg ccctaaagcg taatgacgat aacagctgga ccgtcaccgt tgccgatctg
    38341 aaaaacggca ctgcacagaa catccgcgcc aaatttgtct ttatcggcgc gggcggtgcg
    38401 gcgctgaaac tgttacagga atcggggatt ccggaggcga aagactacgc cggtttcccg
    38461 gtgggcggac agttccttgt ttcggaaaac ccggacgtgg ttaatcacca tctggcgaag
    38521 gtttacggta aagcatccgt tggcgcacca ccgatgtcgg ttccgcatat cgatacccgt
    38581 gttcttgacg gtaaacgcgt agtgctgttt gggccatttg ccaccttctc aaccaaattc
    38641 ctcaaaaacg gttcattatg ggatctgatg agttccacca ccacctctaa cgtgatgccg
    38701 atgatgcacg ttgggctgga taatttcgat ctggtgaaat atctggtgag tcaggtgatg
    38761 ttgagtgaag aggatcgttt tgaagcgttg aaagagtact atccgcaggc gaaaaaagag
    38821 gactggcgtt tgtggcaagc ggggcagcgc gtgcagatta tcaagcgtga tgccgataaa
    38881 ggtggcgtac tgcgtctggg tactgaagtc gtcagtgacc agcaagggac cattgccgcg
    38941 ctcctggggg catcgccagg ggcgtcaacc gccgcgccga ttatgttgaa tctgctggaa
    39001 aaagtatttg gcgatcgcgt ttccagcccg caatggcagg ctacgttgaa agcgatcgtt
    39061 ccgtcttatg gacgcaagct gaacggtgat gtagcggcaa cagaacgcga gttgcagtac
    39121 accagcgaag tgctggggct gaaatacgac aagccgcagg cagcagatag tacgccgaaa
    39181 ccgcagttga aaccgcaacc cgttcaaaaa gaagtggcgg atattgcgtt gtaatgatgt
    39241 gccacatccg gcatggtttg ccggatgtgg cgtatgctga taagacgctt cagcgtcgca
    39301 tcaggcaacc ggctcgggcg ttagatgtcg gatgcggcgt aaccgcctta ttcggcctac
    39361 aggttgcagc acttcacagc agtttagcga acaaccgcgt tgtcaatttt ctcttccgcc
    39421 ttccagacac ggtacttcac atccacattg tctggcgtat acaccacgat cggcagcttg
    39481 ctgttgtaac gcagcattcc agcatcgccc agatacgcgg tgacaaattt cttctctttc
    39541 ttgccatccg ggcaggccat catcgtggaa accggcgagc taactttatc aaagacataa
    39601 taatcatagc cccagccttc cagcgttttg ctttccagct tcccgccgag acgatgcaaa
    39661 ttgcagtcga cttccagcgt ctgaccgatt aacagttcta ctttcagggt agattcatct
    39721 tcttgcggag ttaactgaat cacctgacgc ttcatgcctt tttcagcttg tggataaggt
    39781 gcgatttttt ccagtggctg gacgctttct gccgcccagg cggaagtggt ggcgaaagcg
    39841 gcaaacaata ctgcaggtag aatggtcttc atttttttgc tttccttctt catttatagg
    39901 tt
//
LOCUS       DAZFFE010000029        39679 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFFE010000029 DAZFFE010000000
VERSION     DAZFFE010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.029"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            84..374
                     /locus_tag="ACHJ69_001751"
                     /pseudo
     CDS             84..374
                     /locus_tag="ACHJ69_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
     gene            557..2818
                     /gene="ybhJ"
                     /locus_tag="ACHJ69_001752"
     CDS             557..2818
                     /gene="ybhJ"
                     /locus_tag="ACHJ69_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO5014752.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGSISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGADDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSEMADVFARIKQIAGQEHVDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPNFDVGDYIYIPGIKAALDNPRTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(2959..4242)
                     /gene="ybhC"
                     /locus_tag="ACHJ69_001753"
     CDS             complement(2959..4242)
                     /gene="ybhC"
                     /locus_tag="ACHJ69_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO5014753.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDKVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(4394..4870)
                     /gene="ybhB"
                     /locus_tag="ACHJ69_001754"
     CDS             complement(4394..4870)
                     /gene="ybhB"
                     /locus_tag="ACHJ69_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO5014754.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPVGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            4979..6109
                     /locus_tag="ACHJ69_001755"
     CDS             4979..6109
                     /locus_tag="ACHJ69_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO5014755.1"
                     /translation="MKRAYKYRFYPTTEQAELLAQTFGCVRFVYNSILRWRTDAYYER
                     KEKIGYLQANARLTALKKEPEYIWLNDVSCVPLQQSLRHQQAAFANFFAGRAAYPAFK
                     SKRHKQVAEFTASAFKHRDGELYIAKSKSPLDVRWSRELPSAPSTVTISRDSAGRYFV
                     SCLCEFEPVSMPVTAKTVGIDVGLKDLFVTDTGFKTDNPRHTAKYAKRLTLLQRRLSR
                     KQKGSRNRIKARLKVARLHAKIADCRMDNLHKLSRKLINENQVVCVESLKVKNMIRNP
                     KLSKAIADAGWSELVRQLQYKGKWAGRSVVAIDQYLPSSKCCSCCGFTMQKMPLNVRK
                     WHCPECGADHDRDINAARNIKAAGLAVLAHGEPVNPESQHAA"
     gene            complement(6220..7509)
                     /gene="bioA"
                     /locus_tag="ACHJ69_001756"
     CDS             complement(6220..7509)
                     /gene="bioA"
                     /locus_tag="ACHJ69_001756"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO5014756.1"
                     /translation="MTTDDLTFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDRDGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEAQLREQLAPACDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            7596..8636
                     /gene="bioB"
                     /locus_tag="ACHJ69_001757"
     CDS             7596..8636
                     /gene="bioB"
                     /locus_tag="ACHJ69_001757"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO5014757.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVREAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            8633..9787
                     /gene="bioF"
                     /locus_tag="ACHJ69_001758"
     CDS             8633..9787
                     /gene="bioF"
                     /locus_tag="ACHJ69_001758"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO5014758.1"
                     /translation="MIWQEKIDAALDARRVADALRRRYPVAQGAGRWLVADDCQYLNF
                     SSNDYLGLSHHPQIIRAWQQGADQFGVGSGGSGHVSGYSVAHQVLEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSNTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLAGSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            9774..10529
                     /gene="bioC"
                     /locus_tag="ACHJ69_001759"
     CDS             9774..10529
                     /gene="bioC"
                     /locus_tag="ACHJ69_001759"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO5014759.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     RVLDAGCGPGWMSRRWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNAVHYQHYIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            10522..11199
                     /gene="bioD"
                     /locus_tag="ACHJ69_001760"
     CDS             10522..11199
                     /gene="bioD"
                     /locus_tag="ACHJ69_001760"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO5014760.1"
                     /translation="MSKCYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPKGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIEALVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAILHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
     gene            11778..13799
                     /gene="uvrB"
                     /locus_tag="ACHJ69_001761"
     CDS             11778..13799
                     /gene="uvrB"
                     /locus_tag="ACHJ69_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO5014761.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(13837..14745)
                     /gene="yvcK"
                     /locus_tag="ACHJ69_001762"
     CDS             complement(13837..14745)
                     /gene="yvcK"
                     /locus_tag="ACHJ69_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO5014762.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKEDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     regulatory      15010..15154
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            15142..16131
                     /gene="moaA"
                     /locus_tag="ACHJ69_001763"
     CDS             15142..16131
                     /gene="moaA"
                     /locus_tag="ACHJ69_001763"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO5014763.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGIELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            16153..16665
                     /gene="moaB"
                     /locus_tag="ACHJ69_001764"
     CDS             16153..16665
                     /gene="moaB"
                     /locus_tag="ACHJ69_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO5014764.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLVFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            16668..17153
                     /gene="moaC"
                     /locus_tag="ACHJ69_001765"
     CDS             16668..17153
                     /gene="moaC"
                     /locus_tag="ACHJ69_001765"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO5014765.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            17146..17391
                     /gene="moaD"
                     /locus_tag="ACHJ69_001766"
     CDS             17146..17391
                     /gene="moaD"
                     /locus_tag="ACHJ69_001766"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO5014766.1"
                     /translation="MINVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHSLTDGDEVAFFPPVTGG"
     gene            17393..17845
                     /gene="moaE"
                     /locus_tag="ACHJ69_001767"
     CDS             17393..17845
                     /gene="moaE"
                     /locus_tag="ACHJ69_001767"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO5014767.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            17982..18686
                     /gene="ybhL"
                     /locus_tag="ACHJ69_001768"
     CDS             17982..18686
                     /gene="ybhL"
                     /locus_tag="ACHJ69_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO5014768.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            18891..19604
                     /gene="ybhM"
                     /locus_tag="ACHJ69_001769"
     CDS             18891..19604
                     /gene="ybhM"
                     /locus_tag="ACHJ69_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO5014769.1"
                     /translation="MESYSQNSNKLDFQHEARILNSIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPQMYVAIGLLLLSMYGLSKDINKINTAIAGVIYLFLISLVAIVVASLAP
                     VSAIIVVFSTAGSMFLISMLAGLLFKFDPGSHRFIIMMTLTGLALVIIVNATLMSERP
                     IWVISCLMIVLWSGIISHGRNKLLELTGKSHSEELWSPVRCAFVGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(19640..20596)
                     /gene="ybhN"
                     /locus_tag="ACHJ69_001770"
     CDS             complement(19640..20596)
                     /gene="ybhN"
                     /locus_tag="ACHJ69_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO5014770.1"
                     /translation="MSKSHPRWRLAKKLLTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGVIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWEFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(20596..21837)
                     /gene="clsB"
                     /locus_tag="ACHJ69_001771"
     CDS             complement(20596..21837)
                     /gene="clsB"
                     /locus_tag="ACHJ69_001771"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO5014771.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVGP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(21834..22595)
                     /gene="ybhP"
                     /locus_tag="ACHJ69_001772"
     CDS             complement(21834..22595)
                     /gene="ybhP"
                     /locus_tag="ACHJ69_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO5014772.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRTHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            22728..23138
                     /gene="ybhQ"
                     /locus_tag="ACHJ69_001773"
     CDS             22728..23138
                     /gene="ybhQ"
                     /locus_tag="ACHJ69_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO5014773.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(23100..24206)
                     /gene="ybhR"
                     /locus_tag="ACHJ69_001774"
     CDS             complement(23100..24206)
                     /gene="ybhR"
                     /locus_tag="ACHJ69_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5014774.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVVYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPMWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(24217..25350)
                     /gene="ybhS"
                     /locus_tag="ACHJ69_001775"
     CDS             complement(24217..25350)
                     /gene="ybhS"
                     /locus_tag="ACHJ69_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5014775.1"
                     /translation="MSNPILSWRRVRALCFKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(25343..27079)
                     /gene="ybhF"
                     /locus_tag="ACHJ69_001776"
     CDS             complement(25343..27079)
                     /gene="ybhF"
                     /locus_tag="ACHJ69_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO5014776.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEIDINETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     NSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHGNE"
     gene            complement(27072..28067)
                     /gene="hlyD"
                     /locus_tag="ACHJ69_001777"
     CDS             complement(27072..28067)
                     /gene="hlyD"
                     /locus_tag="ACHJ69_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO5014777.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQSAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPNKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(28070..28741)
                     /gene="cecR"
                     /locus_tag="ACHJ69_001778"
     CDS             complement(28070..28741)
                     /gene="cecR"
                     /locus_tag="ACHJ69_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO5014778.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLASAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     SDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            28970..30331
                     /gene="rhlE"
                     /locus_tag="ACHJ69_001779"
     CDS             28970..30331
                     /gene="rhlE"
                     /locus_tag="ACHJ69_001779"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO5014779.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGRGQGGGRGQQQPRRAESGAKSGNAKPAEKPSRRLGDAKPAGEQQRRRRPRKPA
                     AAQ"
     gene            complement(30564..30764)
                     /locus_tag="ACHJ69_001780"
                     /pseudo
     CDS             complement(30564..30764)
                     /locus_tag="ACHJ69_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR domain-containing protein"
     gene            30868..33018
                     /gene="dinG"
                     /locus_tag="ACHJ69_001781"
     CDS             30868..33018
                     /gene="dinG"
                     /locus_tag="ACHJ69_001781"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO5014780.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            33046..34008
                     /gene="ybiB"
                     /locus_tag="ACHJ69_001782"
     CDS             33046..34008
                     /gene="ybiB"
                     /locus_tag="ACHJ69_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO5014781.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLMATGEAATISDGLARVNQAF"
     gene            34149..35234
                     /gene="hcxB"
                     /locus_tag="ACHJ69_001783"
     CDS             34149..35234
                     /gene="hcxB"
                     /locus_tag="ACHJ69_001783"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO5014782.1"
                     /translation="MVSGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKVVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQELGIPLDAGS
                     WQAICDAARQVGMPEETLQAFCQQLAS"
     gene            35301..35360
                     /gene="ybiE"
                     /locus_tag="ACHJ69_001784"
     CDS             35301..35360
                     /gene="ybiE"
                     /locus_tag="ACHJ69_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO5014783.1"
                     /translation="MRLSANRQGNTCAERSVNA"
     gene            complement(35463..35723)
                     /gene="ybiJ"
                     /locus_tag="ACHJ69_001785"
     CDS             complement(35463..35723)
                     /gene="ybiJ"
                     /locus_tag="ACHJ69_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO5014784.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(35988..36254)
                     /gene="ybiI"
                     /locus_tag="ACHJ69_001786"
     CDS             complement(35988..36254)
                     /gene="ybiI"
                     /locus_tag="ACHJ69_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO5014785.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(36328..37005)
                     /gene="ybiX"
                     /locus_tag="ACHJ69_001787"
     CDS             complement(36328..37005)
                     /gene="ybiX"
                     /locus_tag="ACHJ69_001787"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO5014786.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPESYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(37047..39329)
                     /gene="fiu"
                     /locus_tag="ACHJ69_001788"
     CDS             complement(37047..39329)
                     /gene="fiu"
                     /locus_tag="ACHJ69_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO5014787.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLITAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGAIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQMIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(39594..>39679)
                     /locus_tag="ACHJ69_001789"
     CDS             complement(39594..>39679)
                     /locus_tag="ACHJ69_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdgH/BhsA/McbA-like domain containing protein"
                     /protein_id="HFO5014788.1"
                     /translation="REQGAKGYVINSAGGNDQMFGTAIIYK"
BASE COUNT         9383 a        10285 c        10697 g         9314 t
ORIGIN      
        1 gggacagctg atagaaacag aagccactgg agcacctcaa aaacaccatc atacactaaa
       61 tcagtaagtt ggcagcatca cccgcttctg gtagtgccta tatcgttgcc atgttaccgg
      121 tatttgccat gctggcgaat gtctccggcg caccgttaat gttaaccgcg ctggcactgt
      181 tgttctctaa ctcctatggc ggcatggtta ctcactatgg cggcgcggca ggtccggtca
      241 tctttggcgt gggttacaac gatattaaat cctggtggtt ggtcggtgcg gtactgacga
      301 tattaacctt cctggtgcat atcaccctcg gcgtgtggtg gtggaatatg ctgatcggct
      361 ggaacatgct gtaaatatac tcgtcatact tcaagttgca tgtgctgcgt ctgcgttcgc
      421 tcaccccagt cacttactta tgtaagctcc tggggattca ctcgcttgtc gccttcctgc
      481 aactcgaatt atttagagta tatccattta ttatctttct gcgcaattca cggtgcgcag
      541 atatctggag catttgatga tcaagttatc tgaaaaaggc gtgtttctcg ccagcaataa
      601 cgaaataatt gccgaagaac atttcaccgg cgaaataaaa aaagaagaag caaaaaaagg
      661 cactattgcc tggtctattc tctcttctca taatacgtcc ggaaatatgg ataaacttaa
      721 aattaagttt gattcattag cctctcacga tattaccttt gttggtattg tacagaccgc
      781 taaagcgtcc ggtatggaac gtttcccgct gccgtatgtg ctgaccaact gccataactc
      841 actctgtgct gtgggcggca ccattaacgg tgatgaccat gtttttggtt tatcggcggc
      901 ccagcgttat ggcggtattt ttgtgccacc gcatattgcg gtcatccatc aatatatgcg
      961 tgaaatgatg gcgggcggcg gcaaaatgat cctcgggtca gacagccata cccgttacgg
     1021 tgcgttaggg acaatggcgg ttggcgaggg cggcggcgag ttggtaaaac agctgcttaa
     1081 tgacacctgg gatatcgact atccgggcgt ggttgcggtg catctgaccg gaaaacctgc
     1141 gccgtatgtg gggccgcagg atgtggcgct ggctatcatc ggtgccgtgt tcaaaaacgg
     1201 ttacgtcaaa aacaaagtga tggagttcgt aggtcccggt gttgcagcgc tctctaccga
     1261 tttccgtaac agcgttgacg tgatgaccac tgaaacgacc tgtttaagtt ctgtctggca
     1321 aaccgatgaa gaagtccata actggctggc gctgcacggt cgcggccagg attactgcca
     1381 acttaaccct caaccaatgg cgtactacga tggcagcatc agcgttgatt taagcgccat
     1441 caaaccaatg attgcgctgc cattccaccc gagcaacgtg tatgaaatcg acacgctgaa
     1501 ccagaatctg accgacattc tgcgtgagat tgaaattgag tccgaacgcg tggcgcacgg
     1561 taaagccaaa ctctcgctgc tggataaagt ggaaaatggt cgcctgaaag tgcagcaggg
     1621 gattatcgcg ggctgttctg gcggtaacta cgaaaacgtc atcgcggcgg cgaatgcgct
     1681 gcgcggtcaa tcctgcggca acgatacctt ctcgttggcg gtttacccgt catcacagcc
     1741 ggtgtttatg gatctcgcca aaaaaggtgt ggtagcagat ttgattggcg caggcgcaat
     1801 catcagaacc gcgttctgcg gcccatgctt tggtgcggac gatacgccaa tcaacaacgg
     1861 tttgagtatt cgccacacca cgcgtaactt cccgaaccgc gaaggctcta agccagctaa
     1921 tgggcagatg tcagcggtgg cgttgatgga cgctcgttct atcgctgcga ctgcggcaaa
     1981 cggtggctat ttaacctctg ccagcgaact cgattgctgg gacaacgtgc cggagtacgc
     2041 cttcgatgta acgccgtata aaaaccgtgt ttatcagggc tttgtgaaag gggcgaccca
     2101 gcaaccgctg atttacggac cgaacattaa agactggcca gaattgggtg cgctgactga
     2161 caatatcgtc ctcaaagtgt gctcgaagat cctcgacgaa gtgaccacca ccgacgaact
     2221 gattccttcc ggtgaaacct cttcatatcg ttcaaatccg attggtctgg cggagtttac
     2281 tctgtcacgc cgcgatccag gttatgttgg cagaagtaaa gcgactgccg agctggaaaa
     2341 tcagcgtctg gcgggaaatg tcagcgagat ggcagatgtg tttgcgcgca ttaagcagat
     2401 tgctggtcag gaacatgttg atccgctgca aactgaaatt ggcagcatgg tttatgcggt
     2461 gaaaccaggc gatggttctg cgcgtgaaca ggcggcgagt tgtcagcgtg tgattggcgg
     2521 tctggcgaat attgccgagg agtacgcgac taaacgctat cgttctaacg tcatcaactg
     2581 ggggatgtta ccgctgcaga tggcggaagt accgaacttt gacgtaggtg attacattta
     2641 tatccctggc attaaagcgg cgctggataa tcctagaacg acgtttaaag gttatgtgat
     2701 ccatgaagat gcaccggtaa cggaaatcac gctgtatatg gaaagtctga ctgccgaaga
     2761 acgcgagatt atcaaggcgg gtagtttgat taacttcaat aaaaaccgtc agatgtaaaa
     2821 agcgccatgt gaatgtaggt cgcatccggc acttattgtc ggatgcgatg ctggcgcatc
     2881 ttatccgacc gacaaatcgt atcgaactgt aggccggata aggcgtttac gccgcatccg
     2941 gcacatagtt aacagctcct acttcttcgc ctctgcaacc actttgctac ccacgccgcg
     3001 gttattgtat tcccacatgc ggttgtagtt agtgtcattc agattgcgct gtacttcgtc
     3061 gttatcatca acgctgccgg tgttacccgc aaacggacga ttagagatca ccgcatcagc
     3121 ccacggtttg gctgtgttaa aaccttcgtt gatggcgcta tcacggatca ctacctgacc
     3181 gttggtattg gcatcaacat ccagcgagcg gcccagttgc gccacgccat caccggaagc
     3241 attgaaacgg ctgtttacgg cgaggaaacc gtagtaaatg ttggacagcg tagccggtgc
     3301 aaacacatac gcttcttgct gggtacggga gttcaccacg cggaattcgg tgttatcgaa
     3361 caccactgcg ccgcgaccag aaacgatatc cacatcccct tcaatatagc tgttggtcac
     3421 cagcgtacgc ggctgacgat tcgtttccag acggttctgc acaccgctgt tggtgacaaa
     3481 gaaggtgttc tgacggccga gaatgttgac gttattgatc tgcactttgt cgccatcagt
     3541 acgcagtgcc accgccggat ggttacccgc atctacgcta tcgcccagcg tgttttcgat
     3601 ggtcaggttt tgcagttgca ggccattgtt ttgtgaccag aagaccgcag agcagagaac
     3661 accgatactg tcgctgcgtt tactctggca gctatcgtac atataccacg ccggtttacc
     3721 tggcatatat ttgccgcgcg ggttgacgtc gtgacgccag tcggcagggc tcatgccacc
     3781 atcaagggaa agcccaatct tcacatcaat cggtttttca cctgtaccgt acagagtaat
     3841 tccacccgga gcggcaggga catacaccgt tccctgatac tcaccaggca tcacggcaat
     3901 atactggcgc ttgttggtac gcttgataat tgccgcatct accgccgcct gaatcgtggt
     3961 atgcgttaca ccttgagtac ccgccgggcc gacaacaaag tcaggttgcg caggcagggt
     4021 aatcggggaa ggattccatg ctgccgcacc tggtgtcagg gatgcaaaat agtgttgagc
     4081 atcgaaattc tgcgcttctt ttgccgacag aatcgggcga gaagaggtac caggcgcggt
     4141 ttgatcagaa ggacgttgat cgggcggtgt tgagctacag gcggtcagcg tcacgccaaa
     4201 agccaatgcc agcgccagac gggaaactga aaatgtgttc acaggttgct ccgggctatg
     4261 aaatagaaaa atgaatccgt tgaagcctgc ttttttatac taacttgagc gaaacgggaa
     4321 ggtaaaaaga caaaaagttg tttttaatac ctttaagtga taccagatgg cattgcgcca
     4381 tctgacagag tgattaacta aacatcgcag taatcgaggc actcgccaga gagtgaaaat
     4441 gaacgttaaa cccgaccatc gcgccgctgg caccttcatc gacatcaata cgttctacat
     4501 ccagcgcgtg aacggtaaaa atgtagcgat gggtttcgcc tttcggcggc gctgcgccat
     4561 cgtatccggt tttaccaaag tcggtacgcg tctgcaaaac gccgtctggc attgctacca
     4621 gaccagagcc aaacccttgc ggtaatacgc gggtatcagc gggtaaatta acaactaccc
     4681 agtgccacca gccggagccg gttggcgcat ccgggtcata gcaggtgaca acaaaacttt
     4741 tcgttcccac aggaacatca tcccacgcca gatgcggtga aatattatcg ccatcgtaac
     4801 ccatgccgtt aaagacatga cgatgcggca gcttatcgcc atcgcgcaga tcgttactga
     4861 tgagtttcat tagaatgcct ccgggaaacc tcggccttca gaccggggag gaaaggaggc
     4921 ggttttccga ctaactgtac tttgcataat cacattttcc tctttagtat gtgaacacat
     4981 gaaacgcgca tataaatacc ggttttaccc gacaactgag caggctgagc ttttagctca
     5041 gacgtttggc tgtgtgcgct tcgtctacaa ttccatcctt cgttggcgta ccgatgcgta
     5101 ctacgagcga aaagaaaaga tcggttatct acaggccaac gctcgcctta cggcgctcaa
     5161 aaaagagcct gaatacatat ggctgaatga tgtttcctgc gttcccctcc agcagtcgtt
     5221 gcgccaccaa caagccgcct ttgctaactt ctttgccgga cgagctgcat atccggcttt
     5281 caaaagcaaa cggcacaaac aggtggctga gttcactgcc agcgcgttta aacaccgtga
     5341 cggcgagttg tatatagcaa agagcaagtc gccgctggat gttcgctgga gtcgagaatt
     5401 accatctgcg ccgtcaaccg ttaccatttc cagagatagc gctggcaggt actttgtttc
     5461 ctgcctgtgt gagtttgaac ctgtatcaat gcctgttacc gctaaaacgg tcggcattga
     5521 tgtgggctta aaagatttat tcgtcaccga taccggattc aaaaccgaca atccccgcca
     5581 caccgctaaa tatgcgaagc gattaacgct gctacagcga cgtttaagca ggaagcaaaa
     5641 aggctcaaga aaccgtatta aagcccgctt aaaggtcgcc cgactccatg cgaaaatcgc
     5701 cgattgccgg atggacaatc tgcacaagtt gtcccgcaaa ctgattaacg aaaaccaagt
     5761 tgtttgcgtc gaatccctca aggtgaaaaa catgatccgc aacccgaagc tgtctaaagc
     5821 aatagctgac gcaggctgga gcgaacttgt tcgccagctc cagtacaaag gcaaatgggc
     5881 cgggcggtca gtggtcgcca ttgaccagta tttaccgtcc tcaaaatgct gtagttgctg
     5941 cggtttcacc atgcaaaaaa tgcctcttaa tgttcgtaaa tggcactgcc ctgaatgcgg
     6001 cgcagaccat gatcgcgaca ttaacgcggc acgtaatatt aaagctgccg ggctggcagt
     6061 gttagcccac ggagagcctg taaaccctga atcgcagcac gcggcttagg ttcggctcgt
     6121 tgaagtggga atccccgtca ttcagggcgg ggagccgtca agagccctcc tttcttgttt
     6181 gcagaaagtg tagccagaaa ccctcacgct gacttcccgt tattggcaaa aaaatgtttc
     6241 atcctgcacc gcacggttaa ccgctgcggt cagacgctgc aactgttgcg ggagaataat
     6301 atagggcggc atcaggtaaa tcagtttgcc aaaaggccgg atccagacac cctgttcgac
     6361 aaagaatttt tgcagcgctg ccatattcac cggacgagtg gtttcgacca cgccgattgc
     6421 ccccagtacg cgcacatcgg caaccatttc ggcgtcacag gcgggggcaa gttgctcgcg
     6481 tagctgcgct tcaatagccg ccacctgatg ctgccagtcg ccagattcaa gaatcgccag
     6541 gctggcgttt gctgctgcgc aggccagcgg attgcccata aaagttggcc catgcataaa
     6601 gcagccggct tcgccgttac tgatggtttc cgcaacctcg cgcgtggtga gcgtggcgga
     6661 aagggtcatt gtgccgccgg ttaaggcttt accgagacac aaaatatccg gcgcgatttc
     6721 tgcatgttca caggcaaaca gtttgccggt acgaccaaat ccggtggcga tctcgtcggc
     6781 aatcagcaag ataccgtcgc gatcgcacat tttgcggatt cgttttaacc attccggatg
     6841 gtacatgcgc atcccgcctg cgccctggac aatcggttca atgatcaccg ccgcgatttc
     6901 atgacgatgc gctgccatca ggcgggcaaa gcccaccata tcgcgctcat cccattcgcc
     6961 atccatgcgg ctttgcgggg cgggagcaaa caggttttct ggcagatagc ctttccacag
     7021 gctgtgcatt gagttgtccg gatcgcacac cgacatcgcg ccaaaggtat cgccatgata
     7081 accattgcgg aaggttagaa aacgctggcg cgcttcgcct ttggcctgcc agtactgcaa
     7141 cgccattttc atcgccactt ctaccgctac ggagccggag tccgcgagaa aaacgcactc
     7201 cagcggttgc ggcgtcattg ccaccaattt gcggcacagc tcaatggcgg gcgcatgggt
     7261 gataccgcca aacatcacat gcgacatagc atcaatttgc gacttcatcg ccgcattaag
     7321 ctgcgggtga ttgtaaccgt ggatcgccgc ccaccaggac gacataccgt caaccaggcg
     7381 tctgccgtca gacaaaatta gctcgcaacc ttcggcgctt gccaccggat aaaccggcag
     7441 cggggaggtc atggatgtgt atgggtgcca gatatggcgt tggtcaaagg taagatcgtc
     7501 cgttgtcata atcgacttgt aaaccaaatt gaaaagattt aggtttacga gtctacaccg
     7561 aattaacaac aaaaaacacg ttttggagaa gccccatggc tcaccgccca cgctggacat
     7621 tgtcgcaagt cacagaatta tttgaaaaac cgttgctgga tctgctgttt gaagcgcagc
     7681 aggtgcatcg tcagcatttc gatcctcgtc aggtgcaggt cagcactttg ctgtcgatta
     7741 agaccggagc ttgtccggaa gattgcaaat attgcccgca aagctcgcgc tacaaaaccg
     7801 ggctggaagc cgagcggttg atggaagttg aacaggtgct ggagtcggcg cgcaaagcga
     7861 aagcggcggg atcgacgcgt ttctgtatgg gcgcggcgtg gaagaatccc cacgaacgcg
     7921 atatgccgta cctggaacaa atggtgcagg gggtaaaagc gatgggtctg gaggcgtgta
     7981 tgacgctggg cacgttgagt gaatctcaag cgcagcgcct ggcgaatgcc gggctggatt
     8041 actataacca caacctcgac acctcgccgg agttttacgg caatatcatc accacccgca
     8101 cctatcagga acgtctcgat acgctggaaa aagtgcgaga agctgggatt aaagtctgtt
     8161 caggtggcat tgtgggctta ggcgaaacgg tgaaagatcg cgccggatta ttgctgcaac
     8221 tggcaaactt gccgacgccg ccggaaagcg tgccaatcaa catgctggtg aaggtgaaag
     8281 gcacgccgct ggcggataac gacgatgtcg atgcctttga ttttatccgt accattgccg
     8341 tagcgcggat catgatgccg acctcttacg tacgcctttc tgccggacgc gagcagatga
     8401 acgaacagac tcaggcgatg tgctttatgg ctggcgcaaa ctcgattttc tacggttgca
     8461 aactgctgac tacgccgaat ccggaagaag ataaagacct gcaactgttc cgcaaactgg
     8521 ggctaaatcc gcagcaaact gccgtgctgg cgggcgataa cgaacaacag cagcgtctgg
     8581 agcaggcgct gatgaccccg gacactgacg aatattacaa cgcggcagca ctatgatctg
     8641 gcaggagaaa atcgacgcgg cgcttgatgc gcggcgtgtt gctgatgcct tgcgtcgccg
     8701 ttatccggtg gcacaaggag ccggacgctg gctggtggca gatgattgcc agtatctgaa
     8761 cttttccagt aatgattatt taggtttaag ccatcatccg caaattatcc gcgcctggca
     8821 gcagggcgcg gatcaatttg gtgtcggtag cggcggttcc ggtcacgtca gcggttatag
     8881 cgtggcgcac caggtgctgg aagaagaact ggccgaatgg ctgggctatt cgcgggcgct
     8941 gctgtttatt tctggttttg ccgctaatca ggctgtcatt gccgcgatga tggcgaaaga
     9001 ggaccgtatt gttgccgatc ggcttagcca tgcctcattg ctggaggctg caagtttaag
     9061 cccgtcgccg cttcgccgtt ttgctcataa cgatgtcact catctggcgc gactgcttgc
     9121 ttccccctgt ccggggcagc aactggtagt gacagaaggt gtgttcagca tggacggcga
     9181 tagtgcgcca ctggaggaaa tccagcaggt aacgcaacag cacgatggct ggttgatggt
     9241 cgatgacgcc cacggcacgg gcgttatcgg ggagcagggg cgtggcagct gctggctgca
     9301 aaaggtaaaa ccagaattgc tggtggtgac gtttggcaaa ggatttggcg tcagcggggc
     9361 agcggtgctt tgctccaata cggtggcgga ttatctgctg caattcgctc gccatcttat
     9421 ctacagcacc agtatgccgc ccgctcaggc gcaggcatta cgtgcgtcgc tggcggtcat
     9481 tcgcagtgat gagggggatg cacggcgcga aaaactggcg gcactcatta cgcgttttcg
     9541 tgccggagtg caggatttgc cgtttacgct tgctggctca tgcagcgcca tccagccatt
     9601 gatcgtcggt gataacagcc gtgcgttaca actggcagaa aaactgcgcc agcaaggctg
     9661 ctgggtcacg gcgattcgcc cgccaaccgt acccgctggt actgcgcgac tgcgcttaac
     9721 gctaaccgca gcgcatgaaa tgcaggatat cgaccgtctg ctggaggtgc tgcatggcaa
     9781 cggttaataa acaagccatt gctgcggcat ttggacgggc agccgcacac tatgagcaac
     9841 acgccgatct acagcgccag agtgctgacg ccttactggc aatgctccca cagcgtaaat
     9901 acacccgcgt actggatgcg ggttgtggac caggctggat gagccgccgc tggcgagagc
     9961 gtcacgcgca ggtgactgcg ttagatctct ctccgccaat gcttgttcag gcacgccaga
    10021 aggatgccgc agaccattat ctggcgggag atatcgaatc cctgccgtta gcgactgcga
    10081 cgttcgatct tgcatggagc aatctcgcgg tgcagtggtg cggtaattta tccacggcac
    10141 tccgcgagct gtatcgggtg gtgcgccccg gaggcgtggt cgcgtttacc acgctggtgc
    10201 agggatcgtt acccgaactg catcaggcgt ggcaggcggt ggacgagcgt ccgcatgcta
    10261 atcgcttttt accgccagat gaaatcgaac agtcgctaaa cgccgtgcat tatcaacatt
    10321 atattcagcc catcacgctg tggtttgatg atgcgctcag tgccatgcgt tcgctgaaag
    10381 gcatcggtgc cacgcatctt catgaagggc gtgacccgcg aatattaacg cgttcgcagt
    10441 tgcagcgatt gcaactggcc tggccgcaac aacaggggcg atatcctctg acgtatcatc
    10501 tttttttggg agtgattgct cgtgagtaaa tgttattttg tcaccggaac ggataccgaa
    10561 gtggggaaaa ctgtcgccag ctgcgcgctt ttacaagccg caaaggcagc aggctaccgg
    10621 acggcaggtt acaaaccggt ggcctctggc agcgaaaaga ccccgaaagg cttacgtaat
    10681 agtgacgcgc tggcgttaca gcgcaacagt agcctgcagc tggattacgc aacggtaaat
    10741 ccttacacct tcgcagaacc cacctcgccg catatcatca gcgcgcaaga gggcaggccg
    10801 atagaagcat tggtaatgag cgccggatta cgcgcgcttg aacaacaggc tgactgggtg
    10861 ttagtggaag gtgctggcgg ctggtttacg ccgctttctg acactttcac ttttgcggat
    10921 tgggtaacac aggaacaact gccagtgata ctggttgtgg gcgtgaaact cggctgtatt
    10981 aatcacgcga tgttgactgc gcaggcaata ctacacgccg gactgactct ggcggggtgg
    11041 gtggcgaacg atgtcacgcc tccgggaaaa cgtcacgctg aatatatgac cacgctcacc
    11101 cgcatgattc ccgcgccgct gctgggcgaa attccctggc ttgcagaaaa tccagaaaat
    11161 gcggcaaccg gaaagtacat caatcttgcc ttaatgtagt catcctgtgc ttggttaaat
    11221 tgtgagcgag atcgcgtctc cgattgactg caatttaacc aattaaattc taaaaaaatc
    11281 acgaaaaaaa ttttacctcc gcatcatgcg gtgaatgtgg gaattgccca ggcggcgggg
    11341 gataggggct gcagacagtt atccactatt cctgtggata accatgtgta ttagagttag
    11401 aaaacacgag gcaagcgaga gaatacgcgg cttgcacgcg aattggcgtt aaagacggct
    11461 caaagaaata tcttttattt tttaacaggt tagataattg caatggcagt cactgaacag
    11521 gcatctcttg ccataaaact gtcatcaatc atcttgacaa atgttaaaaa agccgttgct
    11581 ttggggataa cccggtaagg ccggagtttt atctcgccgc agagtaaatt ttgctcatga
    11641 ttgacagcgg agtttacgct gtaacagaaa tattatggtg atgaactgtt tttttatcca
    11701 gtataatttg ttggcataat taagtacgac gagtaaaatt acatacctgc ccgcccaact
    11761 ccttcaggta gcgactcatg agtaaaccgt tcaaactgaa ttccgctttt aaaccttctg
    11821 gcgatcagcc ggaggcgatt cgacgtctcg aagaggggct ggaagatggc ctggcgcacc
    11881 agacgttact tggcgtgact ggctccggga aaaccttcac cattgccaat gtcattgctg
    11941 accttcagcg cccaaccatg gtacttgcgc ccaacaaaac gctggcggcc cagctgtatg
    12001 gcgaaatgaa agaattcttc ccggaaaacg cggtggaata tttcgtctcc tactacgact
    12061 actatcagcc ggaggcctat gtaccgagtt ccgacacctt cattgagaaa gatgcctcgg
    12121 taaacgaaca tatcgaacag atgcgtttgt ccgccaccaa agcgatgctg gagcggcgtg
    12181 atgtggttgt ggtggcgtct gtttccgcga tttatggtct gggcgatcct gatttatatc
    12241 tcaagatgat gctccatctc acggtcggta tgattatcga tcagcgcgcg atcctgcgcc
    12301 gactggcgga gctgcaatat gctcgtaatg atcaagcatt ccagcgtggt actttccgtg
    12361 ttcgtggcga ggtgattgat atcttcccgg cagaatcgga tgacattgca cttcgcgtgg
    12421 agctgtttga cgaggaggtg gaacgattgt cgttatttga cccgctgacc gggcagattg
    12481 tttccactat tccacgtttt accatctacc cgaaaacgca ctacgtcacg ccacgcgagc
    12541 gcatcgtcca ggcgatggag gagatcaaag aagagctggc cgccagacgt aaagtgctat
    12601 tggaaaacaa caaactgctg gaagagcagc ggctgaccca gcgtacccag tttgatctgg
    12661 agatgatgaa cgagctaggc tactgttcgg ggattgagaa ctactcgcgc ttcctctccg
    12721 gtcgtggacc gggtgagcca ccgccgacgc tgtttgatta cctgcctgct gatgggctgc
    12781 tggtggtcga tgaatctcac gtcaccattc cacaaattgg cggtatgtat cgcggtgacc
    12841 gggcgcgtaa agagacactg gtggagtacg gcttccgcct gccatcagcg ctggataacc
    12901 gtccgctgaa atttgaagag ttcgaagcat tagcgccgca aaccatctat gtttcggcga
    12961 cgccgggtaa ttacgagctg gaaaaatccg gtggcgatgt ggtggatcag gtggtgcgtc
    13021 caacaggctt actcgacccg attatcgaag tgcggccggt agcgacacag gtggatgatc
    13081 ttctatcgga gattcgtcag cgagcggcaa ttaacgaacg cgtactggtt acaactctga
    13141 ccaagcggat ggcggaagat ctcactgaat atctcgaaga acacggtgag cgcgtgcgtt
    13201 atcttcactc agatatcgac accgtcgaac gtatggagat tatccgcgac ttgcgtctgg
    13261 gtgagttcga cgtattggta gggatcaact tactgcgcga aggtctggat atgccggaag
    13321 tttcgctggt ggcgatcctc gacgctgaca aagaaggctt cctgcgttcc gaacgttcgt
    13381 tgatccagac cattggtcgt gcggcacgta acgttaacgg taaagcgatt ctctacggcg
    13441 ataagatcac cccatcaatg gcgaaagcga ttggcgaaac cgaacgtcgc cgcgagaaac
    13501 agcagaagta caacgaagaa cacggcatta cgccgcaagg cttgaacaag aaagtggtcg
    13561 atatcctggc gctggggcag aacattgcca aaaccaaagc gaagggcaga ggaaaatcgc
    13621 gcccgattgt tgaaccggat aatgtgccga tggatatgtc gcctaaagcg ttacagcaga
    13681 agatccatga actggaaggg ttgatgatgc aacacgcgca gaatctggag ttcgaagaag
    13741 cggcgcaaat tcgtgaccag ttgcatcagt tgcgtgagct gtttattgcc gcgtcgtgac
    13801 aagatgacga agccggaata ttcttccggc ttcgtgttaa cctaaagcct gtaatgcctt
    13861 ttccagcgcg ctatgcaaca actggcggtc atgacgatac ggaatatcgc tggcctccag
    13921 cacttcctga atcacaatcc gctctttcac cgccgagaca tcctcttttg gcccgacgat
    13981 gaccgcatca attacctttt taccaacata ctgctccata attgccagct tgctttccag
    14041 cttcagatta gccgcaggta aactcaactc acgccccaga ttgccgatat agaccatcgg
    14101 cgctggcgtg cggcgtaatg cctgtgcgat ttccttcagc agcagaattg gcatcaggct
    14161 ggtgtaaaaa ctgccaggcc caataatgat gagatccgct tcactgatag cgtgaaccgc
    14221 ctcacgcgtt gcgggtacat tcggcgttaa caataactct tgaatcggcg tagttaactg
    14281 gtcgatattg acctcgccgt atacttcatg cccctgatcg tcaatcgcca tcagatcaac
    14341 aggatgctcc gacatcggaa tcaaatgtgc gtccactttc agcagatttc gaatcagatt
    14401 gatcgcttca agaggtcgca cgctaaggtg atccagtgcc tttaacatca ggtttccaag
    14461 gttgtgcccg gaaagttcgc cattaccgcc gaaacgatat tcaaacattg cggaagcgac
    14521 gctgggttca gttatcagct gattgaggca gttgcgcata tcgccccagg caatgccgcc
    14581 ttctgagcgg cgaatacgcc ccgtcgagcc accgttatcg gtggtggtga cgatacccgt
    14641 taaacgagaa cccaaagacg aaagtgatga gagaacgcgt cccagtccat gccctccgcc
    14701 gagagcaacg acacgatcaa gatcagccag cgtacgattg cgcatatagt ttcctggagt
    14761 cagattatcc gcgctacagt agcgcaaatt gcgaggaaac agcaattaac ctgccaatat
    14821 tgaggataca aactattttc ttctacctct aaaggacgat gcacgctatg cctccctgat
    14881 gatgtatatc aaagtaaaac cgccattttc ccttattctg tagcgaaata gcacgatcat
    14941 gacgctatat acatgattac atagcgaaag tgtggatggt aaaaatctca tttacacgct
    15001 agtatcggct taaccactaa acactctagc ctctgcacct gggtcaactg atacggtgct
    15061 ttggccgtga caatgctcgt aaagattgcc accagggcga aggaagaaat gacttcgcct
    15121 cccgtatctg gaaaggtgta catggcttca caactgactg atgcatttgc gcgtaagttt
    15181 tactacttgc gcctgtcgat tactgatgtg tgtaactttc gttgcaccta ctgcctgccg
    15241 gatggttaca agccgagcgg cgtcaccaat aaaggctttc tgaccgtcga tgaaattcgc
    15301 cgggttacgc gcgccttcgc cagtttgggc accgaaaaag tccgtctgac aggtggtgag
    15361 ccatctttac gccgcgactt taccgatatc atcgccgctg tgcgagaaaa cgacgctatt
    15421 cgccagatag cggtcaccac caatggttac cgtctggagc gcgatgtggc gaactggcgc
    15481 gatgctggac ttactggcat caacgtcagc gtcgacagtc tggacgctcg ccagtttcat
    15541 gccattaccg ggcaggataa attcaaccag gtcatggcag gaatcgatgc tgcatttgag
    15601 gccggttttg agaaggtcaa agtcaatacc gtgctgatgc gtgatgttaa tcatcaccag
    15661 ctcgacacct ttctgaactg gatccagcat cgtcccatcc agctgcgttt catcgaactg
    15721 atggaaacgg gcgagggcat cgagctcttc cgtaagcatc acatctctgg tcaggttctg
    15781 cgtgacgagc tactgcgtcg cggctggatc caccaattac gtcaacgcag cgacggtccc
    15841 gcgcaagtct tttgtcatcc ggattacgcc ggagagattg gccttatcat gccgtatgaa
    15901 aaagacttct gcgccacttg caaccgcctg cgcgtttcct ccattggtaa actccatctc
    15961 tgcctgtttg gtgaaggcgg cgttaacctg cgcgatctgc tggaagacga tacccagcaa
    16021 caggcgctgg aagcgcgtat ttcagcggcg ctgcgggaga aaaaacagac ccatttcctg
    16081 catcaaaata acaccggtat tacgcaaaac ttatcgtaca ttggtggcta aaacgtcaaa
    16141 aggagagatc agatgagtca ggtaagcact gaatttatcc cgacccgtat tgctattctt
    16201 acggtttcta atcgtcgcgg tgaagaagac gatacctccg gtcactatct gcgcgattcg
    16261 gcgcaagaag cgggccatca cgttgtcgat aaagccattg tgaaagaaaa ccgctacgct
    16321 attcgcgctc aggtatctgc gtggatcgcc agcgacgatg tacaagtggt gttgattacg
    16381 ggtggtactg gcctgacgga aggagatcag gctcccgaag cgctgctgcc gttgttcgac
    16441 cgtgaagttg aaggttttgg tgaagtgttc cggatgttgt cgttcgaaga gattggcact
    16501 tccacgttgc aatctcgtgc ggtagcgggc gtcgctaaca aaacgctggt tttcgccatg
    16561 ccgggttcga ccaaagcgtg ccgtaccgca tgggaaaata tcattgcgcc gcagctggat
    16621 gcccgtacgc gtccgtgtaa tttccatcca catttgaaga aataagtatg tcgcaactga
    16681 cccatatcaa cgccgctggc gaagcgcaca tggtggatgt ctccgccaaa gcggaaaccg
    16741 tgcgtgaagc gcgcgccgaa gcctttgtca ccatgcgcag cgagacgctg gcgatgatta
    16801 ttgatggtcg ccaccacaaa ggcgacgtat ttgccactgc gcgtatcgcc ggtattcagg
    16861 cggcaaaacg cacctgggat ctgatcccgc tgtgtcaccc gctgatgctc agcaaagttg
    16921 aagtcaattt acaggccgag ccggagcaca atcgggtacg tatagaaacc ttatgccgcc
    16981 tgaccgggaa aaccggtgtc gaaatggaag cgttaaccgc ggcctccgtg gcggcgctga
    17041 ccatttatga catgtgcaaa gcggtgcaaa aagatatggt gattggtccg gtacgtttgc
    17101 tggcgaagag cggcggcaag tcgggtgact ttaaggtgga agcggatgat taacgttctt
    17161 tttttcgccc aggtgcgcga gttggtggga acagatgcaa ccgaagtggc tgcggatttc
    17221 ccaaccgttg aagcgttacg ccagcatctg gctgcgcaga gcgatcgctg ggcgctggcg
    17281 ctggaagatg gcaaattgct ggcagctgtc aaccagacgc tggtgagttt tgaccattcg
    17341 ctgactgacg gcgacgaagt ggctttcttc ccgccggtaa ccggaggtta agatggcaga
    17401 aaccaaaatt gttgtcggcc cgcagccgtt cagcgtagga gaagagtacc cgtggctggc
    17461 tgagcgtgac gaagacggtg cggtagtcac ctttaccggt aaggtgcgca accataacct
    17521 gggcgacagc gtcaaagcat taaccctcga acactatccg ggaatgacag aaaaagcact
    17581 ggcagaaatc gttgatgaag cgcgtaaccg ctggccgctg gggcgcgtca cggtaattca
    17641 ccgcattggt gaattatggc cgggcgatga aattgttttt gtcggtgtta ccagtgcgca
    17701 tcgcagcagt gcgtttgaag ccgggcagtt tattatggat tatctcaaaa cccgcgcacc
    17761 gttctggaag cgcgaagcca cgccggaagg cgaccgctgg gtcgaagctc gggagagcga
    17821 tcagcaggcg gcaaaacgct ggtagtttat tgttagccgg ataaggcacc gctgcgtccg
    17881 gcaatttcac cgctcagtac atccgtttac gctgcgtcga tgcacagcct catcattttg
    17941 cagtatcctt aagatattcc ttatatcttc aggagaaagt catggacaga ttcccacgtt
    18001 ctgattcaat cgtacaaccc cgggctggct tgcaaactta tatggcgcaa gtctatggct
    18061 ggatgaccgt tggcttgttg ctgacagcat ttgttgcatg gtatgcggct aattccgcgg
    18121 ccgtgatgga gctgttgttt actaaccgtg tctttttaat cggtctgatc atcgcgcaat
    18181 tagcattggt tattgtgtta tcagcgatga ttcaaaagtt gagcgcaggt gtaacgacga
    18241 tgctctttat gctttattcg gcgctgacgg gtcttacgct ttccagtatt ttcattgtct
    18301 ataccgctgc ttctatcgcc agtactttcg tcgttactgc cgggatgttc ggcgcaatga
    18361 gcctgtacgg ttacaccacg aagcgtgatc taagcggctt tggcaatatg ctgtttatgg
    18421 cgttaatcgg cattgtgctg gcatcgctgg ttaacttctg gctgaaaagc gaagcattga
    18481 tgtgggcagt cacctacatc ggcgtgattg tctttgtcgg attgacggcg tatgacaccc
    18541 agaaactgaa aaatatgggt gagcagattg atactcgcga cacgtcgaac ctgcgcaaat
    18601 attccattct tggcgcgtta accttgtatc tggacttcat caacctgttc ctgatgttgc
    18661 tgcggatctt cggcaaccgc cgttaattct ttttcatcat caatatcccg gccatgtgtc
    18721 gggatttttt tcttattaaa aacgacactt aacaagtacc aggtataaaa tatgccttat
    18781 ggacgagcgg aagcgttttc tgtatccata aatgcaaaag tattttgtat gcgtggtttt
    18841 aaatttattg atggtgaata ttaatgtttt ttctaaggac aactaaaaga atggaatcat
    18901 actcgcaaaa tagcaataaa ttagattttc agcacgaagc caggatatta aacagcatat
    18961 ggctcattac cgctttgggt ttggtggcaa ccgcagggct ggcctggggc gctaagtatc
    19021 tcgaaattac ggcaacaaaa tatgattcac cgcaaatgta tgttgctata ggattattat
    19081 tactttctat gtatggctta agtaaagata tcaacaagat aaataccgcc atcgcgggcg
    19141 taatatattt gtttttaatc tctttggtgg cgattgtcgt tgcgagttta gcgcctgtat
    19201 ctgccattat cgtcgtgttc agcactgcgg gatcgatgtt tttaatcagt atgctggcag
    19261 gtctattgtt taaatttgat cctggttctc accgttttat cattatgatg acgttgacag
    19321 ggttggccct ggtaatcatc gtgaatgcca cattaatgag tgaacgaccc atttgggtaa
    19381 taagttgctt aatgattgtc ttatggtcag gcattatctc gcatggacga aataagctcc
    19441 ttgaactgac aggaaaaagc catagtgaag aattatggag tccggttcgt tgtgcttttg
    19501 taggtgcatt aacactctat tactatttta tcggcttctt tgggattctt gccgctatag
    19561 ctataacgct tgtctggcaa aggcatacgc ggttttttca ttagccgtaa atgcacatac
    19621 taaagaactt aactatactt cacatcgccg cttcattttt cgcccgtagc ttcttcgcct
    19681 ggctttccag caacaggtaa cagatcagcg ccagcagcag tgggataaag taatacagca
    19741 cacggtaagc gagcagggcg gcgataattg tgcctttgga ggtatgctcc ccagccagta
    19801 gcgcgataaa caccgcttcc agcacaccaa ttcccgccgg aatatgcacg atgacgccag
    19861 caatactact aaccagcaac acgcccagta caaagaaata gttcacgctt tgaccaagta
    19921 acagccagat aatcgctccc attaccatcc agttaacgct ggaaatcagc atctgggcga
    19981 gggcgaattc ccacgaaggc agcaccagct tttgcccttt gatggtcata tggcggtgct
    20041 tcgcgaaagc gcaaaaccac aaataaaccg cgataatcat cagtaagcca ataccgagaa
    20101 tacgcagcgt agtttgatcg acataccagt gatccggcaa ctccaccacg cctgcggtaa
    20161 agataacccc cgctagcaaa atatagccta gccagttggt ggtaatactg agcgaaaaaa
    20221 tccgcgtaat agtgctgccc ggtaacccca gtcgggagta caaacgatag cgcataccaa
    20281 tgccgccgac ccaggtactg agtgtcaggt tgaaggcgta gcagataaac gacaccagca
    20341 tcacctggcg cttcgccagt ttgtgaccgc agtagaaacg ggcgagcagg tcgtagcaac
    20401 cgtaaatcag ataactgacg accaccagcc cgaccgcact aagcagcgca acgcgattgt
    20461 agtcgcggat gaccttccag acctcttccc agtccacttt tttggcgtag accaccagca
    20521 acacaatcac cgcgataaaa aacagccagg tgaggagttt ctttgctaag cgccagcgcg
    20581 ggtgtgattt actcatcagg gttttacccc cgtgttttca gtttctaccc gatcctgcgt
    20641 ttccattgtc ggttgtgcgg ggggaccaac ctgcgccaga cgtggcgtgt gcgccggaag
    20701 ccagcccacc agcgccggga agtggcgtaa aaagtggaac gccagcacgc ttttggtcag
    20761 gttccaccaa gtgcgtttcg gcagcatggt ctcatcgacc tgctgacaat ctgcggcgat
    20821 aatgccgttc agattatcgc gcagcgtctg gttaaaatta cgatcgtgga tgatgacatt
    20881 tgcttcgaga ttcagtgaca aactgagcgg atcgagatta ctggacccta ctgtcgccca
    20941 gtgatcgtcc atcaatgcca ctttgccgtg gagcgggcgg cggcggtact caaacacctg
    21001 aacgccacct ttaaccagat agttatacag caaacgcgcg ccgactctga caatcggcat
    21061 atccggttcg ccctgaatga tcagtttgat ccgcaccccg cgccgtgccg ctttacgcaa
    21121 ggcgtgtaaa aaacgatagc cggggaagaa gtaggcgttg gcgataatca cttcccgccg
    21181 cgcctgagtg agcattttca gatagtgacg ttcgatatca tcgcgatgtt cttcgttatc
    21241 gcgccagacc agcaatacct gcgcttctcc cggctggcgg ttctcttccg ctttatgatg
    21301 acgtcgccac cagcgtcgcg ccgcgctctg tcctggcagg ttttccagct caaactggag
    21361 aatatcttcg acaatcggcc cttcgaggcg aaccgcgtaa tcctgtttgg cctccgggcc
    21421 gtagctggac atatgctcgg cggagtaatt cagcccgcca ataaaggcga tacgcgcgtc
    21481 gatcaccaca attttgcgat gcatccggcg aaacacattg gtgcgcatac caaaaaggcg
    21541 agggcgggga tcgtagtagc ggaacaccac gccagctgcc gtcagttcat tgacaaactc
    21601 atcgctgaga tccggcgaac cgtagccatc cagcaagact tccgctttta ccccgcgttg
    21661 cgctgctgcc aatagcgccg catgcagctg tttgccgacg tcatcctcaa accagataaa
    21721 cgtttcaaga atgatgcgtt cttgtgcctc tccaatcgcc ttaaacaccg cgggataata
    21781 ttgctcgccg ttttccagca actggatctt attgccttcg cgccagctac atttcataaa
    21841 tgaatctccg cacttaaagg ggcatgatca gaaaggtgtc gccatgtccg cagcggcaac
    21901 gcggttggcg cgctggcgct ggcatttttg acgtagatcc tgtccagtcg tagtagagga
    21961 aattgcaccg gaaacgtgcg cgccgggcgt ccgtgggtgc gggtaaaaat ctcatccagc
    22021 ccggcctgca cttttaacgg atgattagct ttttgccgcc agtcgttgaa atcacccgct
    22081 accaataccg gttcgccgtc cggtagctca ttcacccatt cggcgagcat cgcaagctgc
    22141 gcctggcggt gcgcctcacg caggcccaga tgtacgcaca tcacatgaat cgctttcccg
    22201 gtcatcggcg gcacaatgcg gcagtagagc acgccgcgct tttccgcacc atcgaccgaa
    22261 acatcacgat tctcataatg ttcaatggga taacgtgaaa gaacggcgtt accgtgatgt
    22321 ccttccgggt atacggcatt gcgaccgtag gcgaaatcgc tccacatggt gtcggcgaga
    22381 aactcgtagt gcgaggtatc gggccagttt tccacatgca gcggatgaac ttcgtgcgcg
    22441 cccatcactt cctgcaggca aacaatatcg gcgctgacgg tacgcacggc gtcgcgaagt
    22501 tccggcaaaa tgaagcgtcg gttaaacgcg gtaaagcctt tgtgaatatt gatggtgagc
    22561 accttgaacg aaaattgttg tgtttgatcg ggcataattt tcctgtcttt gcctcttatc
    22621 tcattgaaat agtgtagtcg gtgtcacaaa aaggtgcggt cttacggaat tttccgtaaa
    22681 gttcggtact ctgaataagt agagataaat tcttcaggag agaagccatg aagtggcaac
    22741 aacgtgttcg tgtcgcaact ggcctaagtt gctggcagat tatgttgcat ttactggtag
    22801 tggcgctgct ggtggtgggc tggatgagta aaactctggt tcacgtcggc gtaggattat
    22861 gcgcactgta ttgtgtcacg gtagtgatga tgctggtgtt tcagcgccac cccgagcaac
    22921 gctggcgtga ggtggcagac gtgctggaag agctgaccac gacctggtat tttggtgcag
    22981 cgctgattgt gctgtggctg ttgtcccgcg tactggaaaa caactttttg ctggcaattg
    23041 cagggctggc aatccttgcc ggcccggcgg tggtgtcttt gctggcgaaa gataagaagt
    23101 tacatcacct tacgtctaaa catcgcgtac gccgctgacc ctgtcgtggc cgttatcacc
    23161 agtagcggcc acaaactatt ccacacaata tccagactcg catccttcaa ataaatctgc
    23221 ttggtaatgt ccgtaaagtg gcgaataggg ttaatccacg tcagattttg cagccacatt
    23281 ggcatgtttt ccaccggcga aacgtaaccg gaaaggagaa tggcgggcat cataaagaca
    23341 aacacgccga taaacgcctg ctgttgtgtt gaacagagtg atgaaatcaa cagaccgaat
    23401 cccaccagtg ataaaccgta aaccaccatc gtaaagtaga acagcgccag cgatccggcg
    23461 aagggaattt gatacgccca gataccaatc gccagcacaa tggtggcctg gaaggtggcg
    23521 acaattaatg ccggtacggc tttgccgatg aatatctgcc aggtggtgag cggcgaaacc
    23581 agtaactgat cgagcgtacc ttgttcacgt tcgcgggcga cggaaagtga agtgacgatc
    23641 attacgccga tagtggtgat catggcgatc agcgacggca ccacaaacca tttgtagtcg
    23701 agattcgggt tataccagtt gcgtaccacc agctcgctgt tgttgggttt cggttttcct
    23761 tccagcagct cctgctgata atttttgacg atctgttgca ggtagttggc ggcaatttgc
    23821 gcactgttgg agttacgccc gtctaggatc aactgtaacg gcgcggtctg gaaggtatcc
    23881 agtttgcgcg agaagtcagc cgggaaacgc accagtagta acgccttttg tgtgtcgatg
    23941 gtcgggcgga tctcctgcgg gctttttagc agcagcacat gggtaaaggc gctggcgcgg
    24001 gcaaaacgtt gagtaagttc caccgaatgc tcgccgttat cttcatcgta gatggcgatg
    24061 gtggcgttgg tcacttccag cgtggcggcg aacgggaaca ggatcacctg aatcagcacg
    24121 ggtaaaatca gaattgcgcg ggtttgcggt tcgcgcagca gcgactgcaa ctctttgcgg
    24181 attaacgtcc ataagcgatg aaacatgctc ttctccctaa tccagccgac gtttggtttt
    24241 cagccacgtc aggccgataa acatcaccgc cgaagcgatc aaaaacagca cgttcaccac
    24301 cagtaccacc ggaatattcc cggcgaggaa caggctttgc agggtgctga cgaaataacg
    24361 tgcgggaata atgtacgtca ccgcgcggat caccgcgggc atactgtcta tctgaaatat
    24421 aaagccggaa agcataatcg acggcagaaa agccgcgttc agggccacct gagcggcgtt
    24481 gaactggttg cgggtaatcg tggaaatcag cagccccatc cccagggtac tgagtaaaaa
    24541 caggctggag ataaaaaaca gaatcagcag cgacccgcga tacggcacgc cgagaataaa
    24601 caccgacacc agcatacaca gcaacatcgc cagcatcccg agaaagtaat aagggatcag
    24661 cttacacagc agcagctccg tgcgggtaat ctccgtagag agcaaagcct ccatagtgcc
    24721 gcgttcccat tctcgcgcca ccaccagcga ggtgagaatc gcgccgatga ccgtcatgat
    24781 aatggtcacc gcaccgggga taataaagtg ctggcttatc gccgccgggt taaaccagta
    24841 gcgggtttgt acatcaataa gcggttcaaa agtctgcccg ttatcctccg cgcgctgcat
    24901 ctgccagatc tgccagatcc cttcgacata cccctgcaca aagttagcgg tattcggctc
    24961 actgccgtcg gtgatcacct gaatcggtgc ggtggcgttg gcgcgctcca tctgttcggc
    25021 gaaatccacg ggaataacca ccagaccgcg aattttcccc gcctgcattt tggcgatcaa
    25081 ttcctgacgg ttatcgctga tggtggcgtc gatgtagggc gaaccggtca tggtgtgggt
    25141 gaaatccagc gccgcttcgc tacgctgttc cagtaaaatc ccgacccgca gcttgctgga
    25201 gtcgaggtta atgccgtaac caaaaataaa cagcagtagc agcgggatca ccaccgcaat
    25261 cagccagcta ctcggatcgc gaacgatctg ccgcgtctct ttgaagcaca gcgcccgcac
    25321 gcgacgccag gacaggatcg gattactcat tgccatgctc cttatcccag tcgtggatca
    25381 actgaataaa ggcctgctcc attgtgggat cgggttgctc gtcgttggcc gactgcgctt
    25441 tcaaatcatc cggcgtgccg ctggcgatta atttcccgcg gtacaccagg ccgatgcggt
    25501 cgcaatattc cgcttcatcc ataaagtggg tggtgaccat caccgtgacg cctttctcta
    25561 ccatactgtt gatatgtagc caaaattcac ggcgggtgag gggatcaacg ccggacgtcg
    25621 gttcgtcgag aaacagaatg tccggttcat gcatcagcga acaggccagc gccagccgct
    25681 gtttaaaacc taatggcagt tcatcggtcg cgtgggaggc gatactgttc aggccgaacg
    25741 cctcgctcat acgggagatt ttttcgttct gcgcccgacc gcgtaagcca tacacaccag
    25801 agaaaaagcg taaattctgt tcgaccgtca ggttgccgta gagcgaaaat ttttgcgcca
    25861 tatagccgag atgctggcgc gctttaccag aactctcttt cagatccatg cccagcacca
    25921 gcgcctgacc agaggtcggt accagcaaac cgcacatcat cttaaaggtg gtcgatttac
    25981 ccgcgccgtt tggtccgagc aaaccaaaaa tctccccgcg tttaacggca aagttgacgt
    26041 gatcggtggc ggcaaaatca ccaaatttct tggttagttc cttcgcttcg atcaccgtct
    26101 cgccgggtgt gccttctacc gtatgtaata ttgcgcccag cggcgattcc gaggttccgg
    26161 caccgcccag caaatcaata aacgcatctt caaaacgcgg cgtagtttcg ttgatgtcga
    26221 tttccggcat cccgtcggca tggcgaatat cgtctggcgt ggtctctttt ttgaggatca
    26281 gacgtaccga tttcccctga atcatgccgt cgctgacctg cggcagtttc aaggcccgtt
    26341 gcaacagttt gcggttgccc tcgtgcgggc tggtcatcag aaagctgcgt ccggccatag
    26401 tttgagtcag ggccgtcggt tctccctgat acagcagctc gccttcgttc atcagtaaca
    26461 cgtcacggca ctgctcggct tcgtcgagat acgaggtact ccagaggatt aacatccctt
    26521 cgcccgccag ctcatgcacc atctgccaca gttcgcgccg tgagataggg tcaacgccga
    26581 cgccgggttc atcgagcagc aacactttcg gttcgcccac cagggtacag gccaggccaa
    26641 gtttttgttt catcccaccg gagagcttgc ccgccaggcg tccggtaaac gggccaagag
    26701 atgtaaactc cagcaggcga gcaaaagtct gcttccgtgc ctcgccggtg acgctgcgca
    26761 aatccgcgta cagattgagg ttctccatca ccgtcagatc ttcatacagg ccgaatttct
    26821 gcggcatata gccgagcacg gcgtgcagcg cgccgtcgtt tttgatcgga tcaaagccaa
    26881 tcaccgtggc actgccgctg tcgggtttca gtaatcccgc cagcatccgc atcagcgtgg
    26941 ttttgcccgc accgtccggc ccaaccagcc ccgtcacata accggcgtga atggtgcagt
    27001 cgagcggtgc gacggcgggc ttgtccatgc ccggaaagcg tttttccagg ccgttcagcg
    27061 tgataacggc atcattcatg tcctgcctcg tcaccgaatt gcaccgttac cggcattccc
    27121 tggcgtaacg catcatcggc gtcggtcacc acaatacgca ggcgatagac gaggtcggta
    27181 cgcagatccg gtgtttcgac ggttttcggg gtaaattcag cagtcggcga aacgaaacca
    27241 atctgcccgt gatacggttt gttcggacga ccatcggtat aaagcagcac tttgcgcccc
    27301 ggctgggcct ggtcaagatt acgttcatca acataggcgc gtacccacac cggacgcgtc
    27361 agcgagacgg taaataccgt gccaccttca ttaagcaccg tgcctggctc taccgcgcgc
    27421 gttaacagcg tgccatcaga cggtgctatc aacgttgagt cctgcaaatt cagctccgcc
    27481 tgcgccagtt gcgcctgcgc ttgttcgagg ctggctttcg cctgggcgat gtcctgttcg
    27541 cggttaccgg aacggtactg acgcaattta tcctgcgctg atttcagcgt ggcctgcgcc
    27601 tggtcgcgcg aggagcgggc attttccagg tcatttgccg aaatggtgcg gcttttccac
    27661 agtccttgct ggcggttata gaagttctgc gcatagtcat aggcagcttg cgcctgtttc
    27721 accgctgcgg cggactgagc gatttcttca tctcgatacc cggcaagcat caggtcatac
    27781 tgcgcctgag ccaccgaaac acccgctttc gcctgcatca gggcaatctc atacggcttg
    27841 tgatccagtt cgcccagcac ctggcccgct ttgatcgcat caccttcgtc caccgccagc
    27901 gactcaacgc gccccccaac acggaaacta agattcaccg tacgaatatc cacgttgcca
    27961 tacagcgtca ggccgttatc ctggcggctt tgataccacc agtagcctcc ggcaaccacg
    28021 gcggcaagta ctactaccgc cagtccgatc acgacaggtt ttttcatcac tacaaactcc
    28081 tttgcgataa tccttgcaaa atcaggtcaa tatgacaggt caccgtctgg ttgatcagtt
    28141 cggtcttttc ttcatcgaac gcggtccagc cggtacgtaa cagaatagtt tctttgccaa
    28201 gacgaaacgc cagaatctca ccaatcaacg catgggtatg aaggatcatg cgggtatcgc
    28261 tagcgtcgca gccggtccag gcggcaatca ggcgtgtaag gtgactgtgt agcggactaa
    28321 tcacctgttc gtgcaccagg tggtaggctg ccgtgggaga gagctgctca cgggagataa
    28381 atttgctgag gttgacggtg tcatcctggg tgagcagctt aatcatgttt ctgcaggcgc
    28441 gaaggatcag ttcacggatg gctgcccgat ccggctgtgg ttgtgcgaac aaacgttcgg
    28501 cttcttcggc gtgtggacgg aactgctcgc caataaaatc agcaatccac tgggcgctgg
    28561 cgaggtacaa atcttctttc gaaccgaagt agtaagtgat ggctgcgata ttctgcccgg
    28621 cctgggcggc tatctcgcgg gtggtggcgt tcattccata ttcaccaaac tgtgccagtg
    28681 ccgcagcaat cagctgtttt ttcgcctgtt cacccttgat tgtcatggca ggattattca
    28741 tcgcacagtc cattcttaat caaatgattg attaagatta tgactccata ggggagttgt
    28801 ccagtatggc taagaatttt agctacgcca gtcacaggga taatttatgc gctgcgtcac
    28861 aaaaactgct acactccgct ccctcatgac attgtggttt ttgtcatttt ccttttcagt
    28921 atctccctga aaactacacc ggtaacggtc ggggcggttc ggagtagtta tgtctttcga
    28981 ttctttgggt ttaagccctg atatcctgcg cgccgttgcc gagcagggtt accgtgaacc
    29041 cacccctatt cagcagcagg cgatccctgc ggtgctggaa ggccgcgacc tgatggccag
    29101 cgcccagacc ggtaccggca aaacagcggg ctttacgctg ccgctgttgc aacacctgat
    29161 cactcgccag ccgcacgcca aagggcgtcg tccggtacgc gcgctcattc ttaccccgac
    29221 ccgtgaactg gcggcgcaga ttggcgaaaa cgtccgtgat tacagcaaat atctgaacat
    29281 tcgttcgctg gtggtgtttg gcggtgtcag cattaaccca caaatgatga agttgcgcgg
    29341 cggcgttgat gtgctggtgg caacgccggg acgtctgctg gacctggaac atcaaaatgc
    29401 agtgaagctg gatcaggttg aaatcctcgt ccttgatgaa gctgaccgca tgctcgacat
    29461 gggctttatc cacgatatcc gtcgcgtgtt aacaaaactg ccagcgaagc gccagaacct
    29521 gttgttctcc gcgaccttct ctgacgatat taaagccctg gcggaaaaac tgttgcacaa
    29581 cccgctggaa atcgaagtgg cacgccgcaa tactgcgtct gatcaggtga cccagcacgt
    29641 tcactttgtc gataagaaac gcaaacgcga attgctgtcg catatgattg ggaaagggaa
    29701 ctggcagcag gtgctggtgt ttacccgtac caaacatggc gctaaccatc tggctgaaca
    29761 gcttaataaa gacggcatcc gtagtgcggc gatccacggt aataaatcgc aaggtgcgcg
    29821 tactcgtgcg ctggccgatt ttaaatcggg cgatattcgt gtactggtgg caactgacat
    29881 cgctgcgcgc ggtctggata tcgaagagtt gccgcacgtc gtcaactatg agttaccaaa
    29941 cgtaccggaa gattatgttc accgtatcgg gcgtactggc cgtgcggcgg caactggcga
    30001 agcattgtcg ctggtgtgtg ttgatgaaca caaactgctg cgtgatatcg aaaaactgct
    30061 gaaaaaagag atcccgcgca ttgcgattcc ggggtatgag ccggacccgt caatcaaagc
    30121 cgaaccgatc cagaacggtc gccagcaacg tggcggcggt cgtggacagg gcggtggtcg
    30181 cggtcaacag caaccacgcc gtgcggaaag tggtgcgaag tctggtaacg cgaagcccgc
    30241 agaaaaaccg tctcgtcgtc tcggcgatgc caaaccggca ggcgaacaac aacgtcgccg
    30301 ccgtccgcgt aaacctgccg ctgcgcagta atcttttatg ccgggctatg cccggcatga
    30361 gactgatgac aaacacaaaa ctgcctgatg cgctacgctt atcaggccta cgtgaactct
    30421 gcaatatatt gaatgtgcat gtttttgtag gccggataag gcgctcgcgc cgcatccggc
    30481 atttcacaac aagcacttgt cagccatttg cgagcgcggc aaaaagattt ctctgtttta
    30541 acgcccacaa gccacccgat gcgttacttc tccgcagcca attgtccgcg caacgccatt
    30601 aaaaggtacc ccaggcgatt cttgccctga ccattaccgc catctcccca gtaagcatca
    30661 ttttgcgtat gctcaaccag ttttgcggac gcggttgcca gcaagagtgt acgcagttct
    30721 gcatgttgtt cgaatttagc gcgaagcgct ttacgcatca tcctgctggt tattgcgagc
    30781 cgctttccag aaacagaaaa accattaccc ctgaaaaccg aaaaatgcca caatattggc
    30841 tgtttataca gtatttcagg ttttctcatg gcattaaccg ccgcgcttaa agcgcaaatt
    30901 gccgcctggt ataaggcgct tcaggaacag atccccgact ttattccccg tgcgccgcag
    30961 cggcagatga ttgcggacgt cgccaaaacg ctggccggag aagaagggcg gcatctggcg
    31021 attgaagccc ctaccggcgt tgggaaaacg ctctcctatt tgatccccgg catcgccatt
    31081 gcccgcgaag agcaaaaaac gctggtggta agtaccgcca acgtggcatt gcaggatcag
    31141 atctacagca aagatttacc gctgctgaaa aagatcattc ccgatcttaa attcactgct
    31201 gcttttgggc gtgggcgcta cgtttgcccg cgtaatctga cagcgctcgc cagcactgag
    31261 cccacgcaac aggatctgct ggcgtttctt gacgacgaac tgacgccgaa caatcaggaa
    31321 gagcaaaaac gttgtgcgaa gctgaagggc gatctcgaca cttataaatg ggatggtctg
    31381 cgcgatcata ctgatatcgc tatagatgat gatctctggc gtcgtttgag taccgacaag
    31441 gccagttgcc ttaaccgcaa ctgttattac tatcgcgaat gcccgttttt tgtcgctcgt
    31501 cgggaaattc aggaagcgga agtggtggtg gcaaaccatg cgctggtgat ggcggcgatg
    31561 gaaagcgaag ccgtattgcc tgacccgaaa aatttactgt tggtgctgga cgaaggtcat
    31621 cacctgccgg atgtggcgcg ggatgcgctg gagatgagcg ccgaaatcac tgcgccctgg
    31681 taccggctcc aactggacct gttcaccaaa ctggtcgcca cctgcatgga gcagtttcgc
    31741 ccgaagacca tcccaccgct ggcgatccct gaacgtttga atgcgcattg tgaagagctg
    31801 tatgagctta tcgcctcgtt aaacaacatt ctcaatctct acatgcctgc cgggcaggag
    31861 gcagagcacc gttttgcgat gggcgaactg cctgatgaag tgctggagat ctgccagcgg
    31921 ctggcaaaac tcaccgagat gctgcgtggt ctggcggagt tatttcttaa cgatttaagt
    31981 gagaaaaccg gcagccatga cattgtacgc ctgcatcggt tgattttgca gatgaaccgc
    32041 gcgttgggga tgttcgaggc gcaaagcaaa ctctggcggc tggcgtctct ggcgcaatct
    32101 tccggtgcgc cggtgaccaa atgggcgacg cgggaagagc gcgaagggca gctacacctt
    32161 tggtttcact gcgtgggaat acgtgtcagc gatcagctgg aaaggctgct gtggcgcagt
    32221 attccgcaca ttattgttac ctccgcaacc ttgcgttcgc tgaacagttt ttcgcgtttg
    32281 caggagatga gtgggctgaa agagaaagcg ggcgaccgtt ttgtggcact ggattcccct
    32341 tttaaccact gtgaacaggg caaaattgtt attccccgga tgcgcgttga gccttccatc
    32401 gacaacgaag agcagcacat tgccgaaatg gcggcctttt tccgtgaaca ggtggagagc
    32461 aaaaaacatc tcggtatgtt ggtgctgttt gccagcgggc gtgcgatgca gcgctttctc
    32521 gactatgtga cggatttacg cctgatgttg ttggtgcagg gcgatcagcc acgttatcgc
    32581 ttagttgaac tgcaccgcaa acgcgttgcc aacggtgagc gtagcgtgct ggtgggctta
    32641 cagtcctttg ccgaaggtct tgatctgaaa ggcgatctgt taagccaggt gcatatccac
    32701 aaaatcgctt ttccgcccat cgacagcccg gtggtgatca ccgaaggtga atggctgaaa
    32761 agcctcaacc gctatccgtt tgaagtacaa agcctgccga gcgcctcgtt taacctgatt
    32821 cagcaggttg ggcgactgat tcgaagccac ggttgctggg gcgaagtggt gatttacgac
    32881 aaacgcttgc tgaccaaaaa ttatggcaag cgactactgg atgcattacc ggtatttccg
    32941 atagagcaac cggaagtccc tgaaggtata gttaaaaaga aagaaaaaac gaaatcccca
    33001 cgccgtcggc ggcgttaatg atgtgagtca ggtaaggagt cgtaaatgga ctatcgcaaa
    33061 atcattaaag agattgggcg cgggaaaaac cacgcgcgcg atttagaccg ggatactgcg
    33121 cgcggtctgt atgcccatat gctcaatggt gaagtccctg accttgagtt gggcggcgta
    33181 ttgattgcgc tacgtatcaa aggggaaggg gaagcagaga tgctcggctt ttacgaagcc
    33241 atgcaaaatc acaccatcaa gctgacgccg cctgcaggca agccgatgcc gattgtcatc
    33301 cccagttaca acggcgcgcg taaacaggcc aacctgacgc cgttgttggc gattctcctg
    33361 cacaaactcg gttttcccgt ggtggttcac ggggttagcg aagatccaac ccgcgtgctg
    33421 acagaaacca ttttcgaact gatgggtatt acgccaacgt tgcacggcgg gcaggcgcag
    33481 gcgaagctcg acgaacatca accggtgttt atgcctgtcg gcgcgttttg cccgccgctg
    33541 gaaaaacaac tggcgatgcg ctggcggatg ggcgtgcgta acagtgcaca taccctggcg
    33601 aaactggcga cgccatttgc cgaaggtgag gcactgcgtc tttccagcgt ttcgcatccg
    33661 gaatacattg gacgcgtcgc gaagttcttt agtgatatcg gcgggcgggc gctactaatg
    33721 catggtactg aaggtgaagt gtatgctaat ccgcagcgct gcccgcagat caatctcatt
    33781 gaccgtgaag ggatgcgggt actgtatgaa aaacaggaca ctgctggtag cgagttactg
    33841 ccacaagcaa aagatccgga aaccacggcg cagtggattg agcgttgcct tgctggcagc
    33901 gaaccgattc ccgaatcgct gaaaatccag atggcttgct gcctgatggc tacgggtgaa
    33961 gcggcaacta tcagcgacgg cctggcgcgc gttaatcagg cattttaatt tttatctcag
    34021 ccggatgagc catgtcatcc ggcttttacc ccaccgcaat atgaaattcc tgcatcttta
    34081 ttgaccttcc cgcgcccggc gtgcagcata aaaaaacaac aaacacataa cataaacagg
    34141 agttaaccat ggtaagtggt catcgctttg atgctcagac gctgcacagt tttattcagg
    34201 ctgtatttcg tcagatgggt agtgaggaac aagaagcgaa attagtcgcc gatcatttaa
    34261 ttgcggcaaa cctggcaggg catgattcac atggtattgg catgatccca agctatgtgc
    34321 gctcctggag tcaggggcat ctgcaaatta accatcatgc caaagtcgtt aaagaggcgg
    34381 gggcggcggt cacgctcgat ggcgatcgcg catttggtca ggtcgcggca cacgaagcga
    34441 tggcgctggg gattgagaaa gcgcatcagc acggcattgc cgccgtggcg ctccataact
    34501 cgcatcatat cggccgtatc ggttactggg cggagcagtg tgcagcggcg gggtttgtct
    34561 ctatccactt tgttagcgtg gtcggtattc caatggtcgc gccgttccac ggtcgcgaca
    34621 gccgctttgg caccaatccg ttctgtgtgg ttttccctcg taaagataat ttcccgctgt
    34681 tgctcgatta cgccaccagc gccattgcat ttggcaaaac ccgcgtcgcc tggcataaag
    34741 gcgtccccgt gccgccaggt tgcctgattg acgttaacgg cgtgccgacg acaaatccgg
    34801 cggtaatgca ggagtcgccg ttgggttcgc tgttgacctt tgccgaacat aaaggctacg
    34861 cccttgcagc gatgtgtgaa attcttggcg gggcgctttc tggcggtaaa acgacgcatc
    34921 aggaaacgtt acaaaccagt cccgatgcca ttcttaactg catgaccact atcatcatca
    34981 acccggaact gttcggcgcg ccggattgta gtgcgcagac cgaagccttt gccgagtggg
    35041 tgaaagcctc gccgcatgat gacgataagc cgattttgct cccgggcgag tgggaagtga
    35101 acacgcgtcg cgaacggcag gagttgggga tcccattgga tgcgggaagc tggcaggcca
    35161 tttgtgatgc ggcgcggcag gttggtatgc cggaagagac gttgcaggct ttctgtcagc
    35221 agttagccag ctaaaaaaaa gcccgtccag tggcggacgg gcaaacaagg gtaacatagg
    35281 atcaatgagg gttagagcat atgcgcctgt cggcaaacag acagggaaat acttgtgctg
    35341 aacgtagcgt aaacgcctga tccagccaac ggttcggatt ttgtaggtct gataagacgc
    35401 ggtagcgtcg catcaggcat ctgttgccgg atgcggcgta aacgccttat ccgtcctacg
    35461 aattacttat aaattaccgc agtaccgctt aatttattgt tgttggtggc agaagtgatg
    35521 ctgtatccac tggcaccggc cgccgcggct ttctcagcca gtttcgcttc cagggcatcg
    35581 agggtggatg cgccatcggc agaaaccacg ccgattttat tcatgttctg tgcctgggat
    35641 gccgttaccg gttccgctgc gaacacgcca aatgacagag ttgaaagagc catagcagca
    35701 acaacagtat tgatagtttt cataattaat ctctcgcagg tgatttttgt ataagtgacg
    35761 ttgtttcgtc gatgtgatga gtatcacgtt tttttacgag agataaaatc gaagagaatt
    35821 gaccgccttg ttcaaataaa ttgattgata tctaacatac tgataaacca tttatttgtt
    35881 gtatttatga atccgttttg taccgcgtgg tgctcacggc agcgttaaag gcacggaatg
    35941 ccacgcaaaa tacaaattac actcactaaa catcccgtca gttgcagtta acgtaactgg
    36001 ctgtctttcg aacctctgcg attatatcct gtataagctg gtttttgtaa atctttctcc
    36061 tgttggcaat gaatgcataa gcgcacgcca ggaatggctt cccgacgggc ctgcgggata
    36121 ggggcaccgc actcttcaca ttcatacagg ctttcgccac gcggaatttc accccgagcg
    36181 cgggcaatcg catcttcaat tgtactgttg atctgttcgt tgacggcgtc atcattagcc
    36241 caaccggatg ccatatcgtc ctccccatat caatacttgt acagttaagt gtagctaata
    36301 tagggacgaa ctcgggcagt tcaagcgtca gatctccgac cattcccgca gcagattatg
    36361 ataaagattg agcagtgaca ggatctcttc actttcgccg tagcggcttt tcagcgactg
    36421 aatattgttg tccagttcaa acagcatggc gcgcttttta tcatcacgga tcatcgactg
    36481 gatccacata aatgatgcca ctcgcacgcc gcgggttacg ggtgtcacgc aatgcaggct
    36541 gctggaggga tacaacacca gatcgcctgc cgggagtttt acccgatgtt gcccgaaggt
    36601 gtcattaacg accagttcgc cgccgtcgta gctttctgga tcgcttaaaa acagcgtggc
    36661 agaaaggtca gtgcgcatcc agccgttttg tggatggctg cgtactgcgc catccacatg
    36721 aaaaccatag gtttcattgt tctgatagcg attaaacagc ggcgtggaaa gggtacgcgg
    36781 caaggccgcg gcaaagaaca aagcatgttg gttaaccgcg ttcagcacct cattttgcag
    36841 ggcggcgtac agggcgctgc gggtgtctac ttgttgattg ttcttaactt gcgcaccttg
    36901 tgcgccggtg gtgacgcgcc catccaccca ttcggcttgt tccagttgtt cgcgaaaacg
    36961 ggcgacgtcc tgtggcgata gcacgccggg aatgtggtac atcatacaag ttctccaaaa
    37021 agtggggcct gcgccccaca tctcaatcag aaatgcatat tggccgtgag caagaaggtt
    37081 cttggctcgc ccgggtgata acggtagccg ctcttattga ttgaggcgac gtaatcggta
    37141 tcaaacaggt tgtagacgtt tagctggaag tcgagattgc gattaacccg ataccccagt
    37201 ttggcgtcgg cgacccaata accttcggta aacgctggcg ttcccaccgc gccgtctgaa
    37261 cctttatgca tactgccgat ataccgtgcg cccgcaccaa cagaaatatc atcggttgcc
    37321 tgatactggc tccataaggt gaaggcgtgt tccggggtat acggcagcga tgaggaacca
    37381 tcctgggcaa catctttgcc gtttttgata gttgcttttt gctgggtata gccgccaatc
    37441 atctgccatg cgggggtgat attcccggcc acggatatct cgtagccttc gacacgtttc
    37501 ttaccatatt gcgaataagt cccgtcatca ttttgctcaa cttcattttc aatatcagta
    37561 cggaacagcg cggcggtaag tagcaggcgt ttatccagaa cctgccattt ggtgccaatc
    37621 tcgctggtgt tggctttttg tggtttaaaa tcggtgcggt tggcactgtt accgctgcca
    37681 gactgcgcaa gggcgaagtt gttgccgccc ggcggctgct gggaaacggc atagttaata
    37741 tagacattgc cgttttccgt caggtgatac agcgccccgg ctttccagtt caccagattg
    37801 cccgacttgg cggtgtcgac ggtggtgacc ggagaacctt ttgccacgcc agcagggcag
    37861 gtgatggcac cgcgcccgct gccgccgcag gcggtagcac tgtcatattc agtatgataa
    37921 ttatccagac ggatcccgcc gttcagctca aaatcacggg tgatttgcag cgtatcgaag
    37981 gcataaattg cgaaggtatc cgtctgacca ttggcgtttg cgccgttgcg cgtcaggccg
    38041 ccgggatgaa tgctgctgtc aggatgataa atatttaccg ccggtaacgt caccggatta
    38101 acgccatagt tagtctgtgt ttcgcgggta aattccacgc cggtactgac atcatgaccg
    38161 atagcgccgg tatagaacgt cgaggtcagg ttggtctggt tggtgagaat tttgttactc
    38221 acatctttgg tattcgccgt acgtgaccac gtccagctat tcacatcgct ggtgggctgg
    38281 gtaatattcg acgctccacc cataatcgcc gttatcaggt aatcctgctt tacgcgcgac
    38341 caacgcgtag tgttgcgaat ggtggtgtta tcgttgatgt cgtgctcaaa acgcatggta
    38401 gcggtgtcgg tggtcgaatc gtcgtaatcg gaatccgtgc cgtaaaagtt atgagtatca
    38461 acttttccgg aatgattcag ggccgctgtt cccgcagatg gggcagaata acccggtaaa
    38521 ccgatggtcg gaatgccgcc gtctggcgtg ttgtgttggg tgacatgcag ataattaaga
    38581 tacaaacgat tcgcggtacc aaggccaaaa gcgacagaag gggcgacgcc gtaacgctca
    38641 tttttgactt tgtcgcgtcc ggcatcgtgc gttttttcgc ccattacatt caggcgcacc
    38701 gcagtggtat caccaatgac ctgattgacg tccagcgtgc cgcggcggaa ccaggcgctg
    38761 ccaatactgg cggaggcgtc aatgccggaa tcattgcgcg gctgtttgct gatcatattg
    38821 atcgagcccg tcggcgcgct gcgcccgtag tcggtgccgg acggcccttt aattacttcg
    38881 acctgctcgg tattgaaggt gtcgcgcgag acgctgccga tatcgcgaat gccatcgata
    38941 taaatactgt tagaggtatc ggcaccgcgc atataaatgg cgtcgccagt ggtggagtta
    39001 ccgttctcac ccgcaaaaaa cgcacccacg ccggggacgt ttttgagcgc gtcggtaagg
    39061 ttggttgcac cctgatcttt aatcacttgt tcagaaatca ccgtcatcgt gcgggtagta
    39121 tccgctaccg gacgcgagaa tttcggatcg gcagattgtt gtggcgcata aagcgaaggc
    39181 gttgatgctt cgacaaccag cgtgtcatcc gcgttagctt gcccttcggc agcgagtgcc
    39241 tgcgccacag gtgtaatacc aatacaaaga ccggcaaaga acgtgagcga atgaaattgt
    39301 ctggcaggga aattgcgatt gttttccatt ttgcaggtga ctttttctta tatggtgaaa
    39361 ttttcactgt cacgagaaag catctctgcg gatgcgatgc cactgttaat cccgtgatag
    39421 cgtccgtgca agtgataata attttgataa gcattatcac ttggcgcgat tttctatcac
    39481 ccattgcgcg aaaaagaaat acatttattt acattgcgtt catatttgat gttttttgct
    39541 tacaaatcgg acgaaaactg catccagagg cgaatgcagt tacgttggtg gggttatttg
    39601 tagatgattg cagtaccgaa catctggtca tttccgcctg cggaattaat gacataacct
    39661 ttcgcgcctt gttcgcgcg
//
LOCUS       DAZFFE010000030        39607 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFFE010000030 DAZFFE010000000
VERSION     DAZFFE010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.030"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(39..230)
                     /gene="yfgG"
                     /locus_tag="ACHJ69_001790"
     CDS             complement(39..230)
                     /gene="yfgG"
                     /locus_tag="ACHJ69_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO5014789.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            248..400
                     /locus_tag="ACHJ69_001791"
     CDS             248..400
                     /locus_tag="ACHJ69_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014790.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMKVNLIIYND
                     DVIMMS"
     gene            582..2825
                     /gene="pdeF"
                     /locus_tag="ACHJ69_001792"
     CDS             582..2825
                     /gene="pdeF"
                     /locus_tag="ACHJ69_001792"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO5014791.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDSRLISQRLFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAI
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQRALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2864..4405)
                     /gene="ppx"
                     /locus_tag="ACHJ69_001793"
     CDS             complement(2864..4405)
                     /gene="ppx"
                     /locus_tag="ACHJ69_001793"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO5014792.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFHGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4410..6476)
                     /gene="ppk1"
                     /locus_tag="ACHJ69_001794"
     CDS             complement(4410..6476)
                     /gene="ppk1"
                     /locus_tag="ACHJ69_001794"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO5014793.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYELVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQSE"
     gene            complement(6648..7286)
                     /gene="purN"
                     /locus_tag="ACHJ69_001795"
     CDS             complement(6648..7286)
                     /gene="purN"
                     /locus_tag="ACHJ69_001795"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO5014794.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLITSAFDSREAYDRELIHEIDMFAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITVRVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7286..8323)
                     /gene="purM"
                     /locus_tag="ACHJ69_001796"
     CDS             complement(7286..8323)
                     /gene="purM"
                     /locus_tag="ACHJ69_001796"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO5014795.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLKLIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8647..9273
                     /gene="upp"
                     /locus_tag="ACHJ69_001797"
     CDS             8647..9273
                     /gene="upp"
                     /locus_tag="ACHJ69_001797"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO5014796.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            9359..10648
                     /gene="uraA"
                     /locus_tag="ACHJ69_001798"
     CDS             9359..10648
                     /gene="uraA"
                     /locus_tag="ACHJ69_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO5014797.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISMLRPEEVVLDAEDADITDK"
     gene            10743..11444
                     /gene="hda"
                     /locus_tag="ACHJ69_001799"
     CDS             10743..11444
                     /gene="hda"
                     /locus_tag="ACHJ69_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO5014798.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11582..11941)
                     /gene="arsC"
                     /locus_tag="ACHJ69_001800"
     CDS             complement(11582..11941)
                     /gene="arsC"
                     /locus_tag="ACHJ69_001800"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO5014799.1"
                     /translation="MTKQAKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEVLIQAMVENPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11962..13425)
                     /gene="bepA"
                     /locus_tag="ACHJ69_001801"
     CDS             complement(11962..13425)
                     /gene="bepA"
                     /locus_tag="ACHJ69_001801"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO5014800.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANDAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNHDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13638..14699
                     /gene="yfgO"
                     /locus_tag="ACHJ69_001802"
     CDS             13638..14699
                     /gene="yfgO"
                     /locus_tag="ACHJ69_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO5014801.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRPWATSIVLILFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQD"
     gene            complement(14798..15268)
                     /gene="bcp"
                     /locus_tag="ACHJ69_001803"
     CDS             complement(14798..15268)
                     /gene="bcp"
                     /locus_tag="ACHJ69_001803"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO5014802.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(15268..15840)
                     /gene="gcvR"
                     /locus_tag="ACHJ69_001804"
     CDS             complement(15268..15840)
                     /gene="gcvR"
                     /locus_tag="ACHJ69_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO5014803.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDTHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            15986..16864
                     /gene="dapA"
                     /locus_tag="ACHJ69_001805"
     CDS             15986..16864
                     /gene="dapA"
                     /locus_tag="ACHJ69_001805"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO5014804.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            16881..17915
                     /gene="bamC"
                     /locus_tag="ACHJ69_001806"
     CDS             16881..17915
                     /gene="bamC"
                     /locus_tag="ACHJ69_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO5014805.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSEWHELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            18128..18841
                     /gene="purC"
                     /locus_tag="ACHJ69_001807"
     CDS             18128..18841
                     /gene="purC"
                     /locus_tag="ACHJ69_001807"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO5014806.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKQFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            19009..19872
                     /gene="ypfJ"
                     /locus_tag="ACHJ69_001808"
     CDS             19009..19872
                     /gene="ypfJ"
                     /locus_tag="ACHJ69_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO5014807.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            19887..21902
                     /gene="tmcA"
                     /locus_tag="ACHJ69_001809"
     CDS             19887..21902
                     /gene="tmcA"
                     /locus_tag="ACHJ69_001809"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO5014808.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEERWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARQGFDAAAFAALSGTLKAGSWL
                     VLLLPVWDEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTANNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALCLVDEGGLSQELSQAVWAGYRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYIHDLDYLSVSFGYTEELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAVLSDDDWLELAGFAFTHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALFALDDVCTERLRDRITQWQFFH"
     gene            21976..22674
                     /gene="ypfH"
                     /locus_tag="ACHJ69_001810"
     CDS             21976..22674
                     /gene="ypfH"
                     /locus_tag="ACHJ69_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5014809.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQLALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(22755..22955)
                     /gene="ypfN"
                     /locus_tag="ACHJ69_001811"
     CDS             complement(22755..22955)
                     /gene="ypfN"
                     /locus_tag="ACHJ69_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO5014810.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(22983..24110)
                     /gene="dapE"
                     /locus_tag="ACHJ69_001812"
     CDS             complement(22983..24110)
                     /gene="dapE"
                     /locus_tag="ACHJ69_001812"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO5014811.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(24114..24470)
                     /gene="yffB"
                     /locus_tag="ACHJ69_001813"
     CDS             complement(24114..24470)
                     /gene="yffB"
                     /locus_tag="ACHJ69_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO5014812.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAATLMTEMPAIIKRPLLCAPGKPM
                     LLGFSESSYQQFFHEV"
     gene            24851..24910
                     /gene="ypfM"
                     /locus_tag="ACHJ69_001814"
     CDS             24851..24910
                     /gene="ypfM"
                     /locus_tag="ACHJ69_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO5014813.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(25009..28111)
                     /gene="acrD"
                     /locus_tag="ACHJ69_001815"
                     /pseudo
     CDS             complement(25009..28111)
                     /gene="acrD"
                     /locus_tag="ACHJ69_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
     gene            complement(28275..29975)
                     /gene="narQ"
                     /locus_tag="ACHJ69_001816"
     CDS             complement(28275..29975)
                     /gene="narQ"
                     /locus_tag="ACHJ69_001816"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO5014814.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            30181..32160
                     /gene="aegA"
                     /locus_tag="ACHJ69_001817"
     CDS             30181..32160
                     /gene="aegA"
                     /locus_tag="ACHJ69_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO5014815.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAVGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEQQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGMAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLNSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            32228..32803
                     /gene="nudK"
                     /locus_tag="ACHJ69_001818"
     CDS             32228..32803
                     /gene="nudK"
                     /locus_tag="ACHJ69_001818"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO5014816.1"
                     /translation="MTQQITLVKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     SQALDMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            32929..33972
                     /gene="ypfG"
                     /locus_tag="ACHJ69_001819"
     CDS             32929..33972
                     /gene="ypfG"
                     /locus_tag="ACHJ69_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO5014817.1"
                     /translation="MRYRIFLLFFFALLPMSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDVSEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGDQTISLNGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVVVVNPTPTPLSREERNDLLDYGNWRMNGLRCSL
                     DPLRREVSVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(34049..36052)
                     /gene="tkt"
                     /locus_tag="ACHJ69_001820"
     CDS             complement(34049..36052)
                     /gene="tkt"
                     /locus_tag="ACHJ69_001820"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO5014818.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVVHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEKEVALARQKL
                     GWHHPPFEIPKEIYHAWNAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVSARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKILGVKGA"
     gene            complement(36072..37022)
                     /gene="tal"
                     /locus_tag="ACHJ69_001821"
     CDS             complement(36072..37022)
                     /gene="tal"
                     /locus_tag="ACHJ69_001821"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO5014819.1"
                     /translation="MNELDGIKQFTTVVVDSGDIESIRHYHPHDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPIDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            37311..39590
                     /gene="maeB"
                     /locus_tag="ACHJ69_001822"
     CDS             37311..39590
                     /gene="maeB"
                     /locus_tag="ACHJ69_001822"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO5014820.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
BASE COUNT         9406 a        10002 c        10398 g         9801 t
ORIGIN      
        1 ggtgccgtta tgttctgtga gagtggagaa ggtaaccgct aacgttgtac cggtgattcg
       61 acggagagtg tggattgctg agcttctttt ttgctctgat ggtgctgcca ggcaccgacg
      121 gacgagtaga taaaacggcc aaagaagaag ataaagctga tgagcagtac gatacgggtc
      181 atgcgactgt taaatcgatg tcgttttcgc atactggttg cctgactcac aaaaggttcc
      241 ttgaagtatg tcccacgccg tggacggtac ttacattaag gcacaacagg acaaaatggt
      301 caattcttcg ttatgtaaaa aagcgtcagt tgatacatat tttaatgtta tgaaagttaa
      361 tttaattatt tacaatgatg atgtaataat gatgagttaa catgataaac gtaataattc
      421 attaatcagg gttattttgt ttgatatata tcaattggaa ctttcatatg acacttagaa
      481 tcattgctct ctatagtgat gaataatcat cattcgaagt caggtgggat gcctgtctga
      541 atacacctcc ttcaggatgt gggggattct gctgagcatc tatgaaactg aatgcaactt
      601 atataaaaat acgtgataaa tggtgggggc ttccgctgtt cctgccttct ttaatcttgc
      661 ccattttcgc ccacattaat actttcgcgc atatttcttc cggtgaagtt tttctctttt
      721 atctgccact ggcactgatg atcagcatga tgatgttttt cagctgggcg gcattgccag
      781 ggatcgcctt agggattttt gtccgcaaat atgcagagct gggtttttac gaaacgctct
      841 cattaacggc taattttatt atcattatca ttctctgttg gggcggttac agggttttta
      901 ccccccggcg taacaacgtt tcacatggtg atagccgttt aatttcccag cgactattct
      961 ggcagattgt gtttcctgca acgctgtttc tgatactttt ccagtttgct gcgtttgtag
     1021 ggttactggc gagcagagaa aatctggtcg gcgtcatgcc ctttaacctc gggaccttaa
     1081 tcaattatca ggccttgctg gtgggtaatc tgattggtgt cccgctgtgc tacttcatca
     1141 ttcgggtggt gcgaaatccg ttttatttac gtagctatta ttcgcaatta aaacagcagg
     1201 ttgatgccaa agttaccaaa aaagagttcg caatctggct actggcatta ggtgctttac
     1261 tattgctgtt atgcatgccg ttaaatgaaa aaagcacgat ttttagcacc aattacacct
     1321 tgtcattatt gctgcccctg atgatgtggg gagcgatgcg ctatggttat aagctgattt
     1381 cattgctctg ggcggtcgtg ttgatgatca gcatccacag ctatcaaaat tacattccca
     1441 tttatcctgg ctataccacg cagctcacca taacctcctc cagttatctg gtattctctt
     1501 ttattgtcaa ttatatggct gtactggcaa cccgtcagcg agcggtagtc agacgcattc
     1561 agcggcttgc gtatgtggac ccggtggttc atctgccaaa tgttcgcgcc ctgaatcgcg
     1621 cgttacgtga tgccccctgg tctgcgcttt gttatttacg catccctggc atggaaatgc
     1681 tggttaagaa ttatggcatc atgctgcgga ttcaatacaa gcaaaaactt tctcactggc
     1741 tgtcaccatt gttggaaccg ggtgaagatg tttatcagct ttcgggtaac gatctcgcgc
     1801 tgcggctgaa tacagaatcg caccaggagc gcattaccgc actggatagc catctcaagc
     1861 aatttcgttt cttttgggat ggaatgccga tgcaaccgca gattggcgtc agttactgct
     1921 atgtgcgctc gccagtgaat catatctacc tgctgctggg agagctaaat acagtggccg
     1981 aactttccat cgtgaccaac gccccggaaa atatgcagcg tcgcggagca atgtatttgc
     2041 aacgcgaatt gaaagataaa gtcgcgatga tgaatcggct acagcgggcg ctggaacaca
     2101 accatttttt cctgatggcc cagccgatta ccggtatgcg tggtgatgtc taccatgaaa
     2161 ttcttctgcg catgaaaggt gagaatgatg aactgatcag ccccgatagc ttcttaccgg
     2221 tcgcgcacga atttggttta tcgtcgagta tcgacatgtg ggtcattgag catacgctgc
     2281 aatttatggc tgaaaacaga gcgaagatgc ccgctcaccg ttttgctatt aatctgtctc
     2341 caacctcggt atgtcaggct cgttttcctg ttgaagtcag tcagctgctg gctaaatatc
     2401 agattgaagc gtggcaactt atttttgaag tcaccgaaag taatgctctg accaatgtta
     2461 agcaggcgca aatcaccttg cagcatcttc aggaattagg ctgccagatt gcgattgatg
     2521 atttcggcac cggctacgcc agctatgcgc ggcttaaaaa tgtgaatgcc gatctgctta
     2581 aaattgacgg cagttttatc cgcaatattg tgtcaaatag tctggattat cagatagtgg
     2641 cgtcgatttg ccacctggcg cgaatgaaga aaatgcgggt agtggcagag tacgttgaaa
     2701 acgaagagat ccgcgaggcg gtgctctctt tggggatcga ttatatgcag ggttatctta
     2761 ttggtaagcc gcaaccgtta attgatacgc tgaatgaaat cgaacccatt cgcgaaagtg
     2821 cctgaataat gcgggccgac atttctcgtc ggcccgcaaa gcattaagcg gcgatttctg
     2881 gtgtactttc ttcttcaatt ttcaaccgcc agccagccac gccttcccag tattcttgct
     2941 ccttttccag atcaagcagt accagcgcat tctgactaaa ccagtcatgc gggaaacgca
     3001 gtgtccagtg actgtcatca gtaatcagcg tcaatgttgg cggtgtggtg gttgcctgac
     3061 gttgattatt gagtaatacg ccaaggcgca atagctgaat cagtggcagg aactgtttct
     3121 tcttaaacaa ggtaaagcgc ggcagatcgt cgagtttaat cgctttacgg tgatagcgca
     3181 ccagtgtcgc catcatcagc tgctgttcct gattaaaacc cggcaagtcg ctgttttgca
     3241 gaatataagc ggagtggcga tgcaaaccgc tgtggttgat attcaacccg acctcatgca
     3301 gcatggcggc ccatcgcagt agcgcctcca gttgtggatg cgccagcttc ggttgctgtt
     3361 cccgccactg ttcgtacatt tgcatagtgg tatccagcac ccgtcgggcc tgttcgctgt
     3421 cgatgtgata ctggttggcg aggctgctgg cagtgcgact acgcacatcc tgatgacgga
     3481 aacgtccttc catttcatac agtacgcctt cgcgtaacgc cccgtcagaa aggcgcagtt
     3541 cacggatggc taaagcatca aacacaccgc ataaaatcgc cagtccagga acgaagactg
     3601 ttttccgctc ttcggaaaga ccaggtaaac tcagcgatgc gaaattacgg tgccgtaaaa
     3661 cttcttttac cagtttttcc agacgttccg gggtaattat cccgtctttc tcgcccattt
     3721 ccattaacac ttcatgggcg gcttttatgg taccggaagc gcccattgcc acgttccagc
     3781 cctgaattcg aaattgccag gttaacgttt ccagtttttg tgctgccgcc atgcgagcgc
     3841 gctgaaaatt ctctttattg atgaccccgc catgaaaata aagctgggca aagctgacac
     3901 aacccatccg gcggctttca acgagaatcg gttcgaaatt ttcaccaatt accagttctg
     3961 tagaaccgcc gccaatatca ataaccagtt tgcgaccttt ttccggttgg gtatgttcca
     4021 cgcccataaa aatcagacgg gcttcttcat taccggaaat aatttcgatc gggtagggaa
     4081 tgactttttc cgcgcgtttc agaaagtcag tggcgttcag cgcctgacgc agcgtatggg
     4141 taccaactat acagacgctg gcaggagaaa acccctgtag cctttcggca aatagcgaca
     4201 gacagttcaa accgcgcgtc attgcctctt cactcaacat attatctggc cccaggccgt
     4261 ccgccagatg cacccgctgt ttcaggcggc caataatctg catggcacca tctaccacac
     4321 gggctatgac catgtgaaaa ctgtttgaac caagatcgac cgcagcaaac tcctgcggac
     4381 gaggggattt atcgtgtatt ggcatagggt tattcagatt gttcgagtga tttgatgtag
     4441 tcgtaaatcg ccaactgcgc ccgtactttg cggcgattgc cgcggggaac gtagcgatta
     4501 ctgagttctt tatcgatata acgtgctttg accgtatcgc tgaacaatat gtcgatgatg
     4561 tccagcaccc gctgcttcag gcgcggatcg agcagcggcg ttgccacttc aatacgataa
     4621 tcaatattgc gcgtcatcca gtcggcggaa gaaaggtaga cctttttatc gccgccattt
     4681 tcaaaaatat aaacccggtc atgttcaagg taacggtcaa caatactgat ggcacgaatg
     4741 ttgtcgctaa tgccttccag attggggatc agagaacaca ttccgcgcac cagcagatta
     4801 accggtacgc cggagctgga ggccgcatac agacgatcaa ccaggccttt atcgacaagg
     4861 ttatttagct tcagggtgat accactgggc agcccttgct gcgcgttggc gatctcgcgg
     4921 tccaccaatt catacaacag acggcgagag ttttgcggcg acaccattaa ataatcaaat
     4981 gtcaccggac ggtatgggtt ttcaataaag ttgaacaccc gccgtacttc gttagtgatg
     5041 cgcgcatcgg cggtcagcaa cgaatagtca gtataaagac gcgcggtttt ttcgttaaag
     5101 ttcccggtcc cgatgtgtgc gtaacgcacc acttcaccgt tttctttacg tgaaatcagg
     5161 aacagtttgg cgtgaatttt cagccccggc gcagagaaga taacgtgcac gcctgcttcg
     5221 gtcaggcgct tcgcccagtg aatgttggct tcttcgtcga aacgcgcctg taactcaacc
     5281 accacggtga ctttcttacc gttgtgtgcg gcgtggatca tcgagtcgat gatgcgtgaa
     5341 tctttcgcca cgcggtaaat gttaatttta atcgccagta cgctcgggtc gaacgaagcc
     5401 tgacgcagca gttccagtac atgctcaaag gtgtgataag gataatagag caacacatcg
     5461 cgttcgcgaa tggcatcaaa accattgcgg aactgggctt tatcaaacca aatatggcgt
     5521 aaacgcggca gtggtttgtt caccagattg gctttgccga cattggggaa attaataaag
     5581 tctttaaaat tatgataacg accgccgggg acgatggagt cgtagcggga aatagtcagt
     5641 ttttcgcgta acacttcaac cagagcattg ggcatatcgc gctgataaac aaaacgcacc
     5701 ggctcagcag ttaaacgctg cttgagactg gaagacatca actccatcag actggcttcc
     5761 atctcatgca ctaaatcgta ttcggcatcg cgggtcatct tcattgaata ggcattcagt
     5821 gcgtcataat caaagaagcc tttgaaaata tcatcaaggc agtaacgcag aatgttatcc
     5881 agaagaatca tcggcttgcg tcgacgcggc gcttctggcg gtaaattcac aaagcgcggc
     5941 actttatctg atgggatctc cagcagcgcg taacggatgg tatcaccacg gataatttcc
     6001 accgccagat aggtgtaatc atctttcagg aactgcacta agtcagtgtc aggattgatt
     6061 aaaatcggcg taatgtgctg acgcagatac tgcttaaaat aatgacgcag ccagttttgt
     6121 tgattgacgg agagctggcg ttcattaatc aggaagatct ggttgcgcgc catctccagc
     6181 agtagctcgt tgtagaggcc gtcgaattcc tgatcggctt tcagcacccg ggactgaatt
     6241 ttgcccagta aatggcggga atgagagttg gaaccttgtt cttcgctaat aatgatgcgt
     6301 cgcttcagtt cagcgaagcg gactttatag aactcatcaa ggttattgga atagatcccc
     6361 aggaaacgca tcctttcaat cagcgggtta gatttgtccg ccgcttcctg aagcacgcgt
     6421 tcattgaacg ataaccaact gagctctttt tcgatgtata gcttttcctg acccattacc
     6481 acttttactc cgttttattc acgggacact gcctggatat tatggcgagc aatattggca
     6541 gatgtccaac tatgccagaa aagggggaaa aaaactgctg gagcttgcga tttacaggtg
     6601 aattgaaaaa cgccagcggc agagctggcg ctttaactac gggggcatta ctcgtcggca
     6661 gcgtagccct gcggcggcag acgttgacca tccagccacg cggcgttttc gtgcattttc
     6721 agacgaccat cggcaaacca gctaatcacc agtggataaa tagcatgttc ctgagtttgc
     6781 acgcggacgg tgatgtcatc ttccgtatca ccagcaaata ccgggacttt cgcctgtaaa
     6841 ataaccgggc caccgtccag ttcatcggtg acgaaatgca ccgatgtacc gtgctcctca
     6901 tcgccatttt ccagcgcctg acgatgggtg tgtaatccgg gatatttcgg cagcagagaa
     6961 gggtgaatgt tcagcaaacg cccggcatag tgggagacaa acgccgggct gagaatgcgc
     7021 ataaaaccag ccagcacgac cacatcgggt gcgaacatgt cgatttcatg aatcaactcc
     7081 cggtcatagg cttcacgact gtcaaacgcg ctggtgatga gcgtatgcgt tgcaataccc
     7141 gcctggcggg cgcgttcaag gccgaacgcg tcggccttat tgctgaaaac tgcccgtacg
     7201 gtgcctttaa ttttgttggt tttgcaggcg tcaataattg cctgcaaatt acttccgttg
     7261 ccggaaataa gcaccacaat attcattatt cgataaccac gcgttgttcg gaatcagagg
     7321 ctttgatgat accgattttc cacgcgtttt caccgttggc attgagcagg gcgagggctt
     7381 tgtccacttc cggagcaggc agggcaataa tcataccgac gccgcagttg aaggtgcgat
     7441 acatttcatg gcgctcaacg ttacctgccg tttgcagcca gttaaacact tccggccact
     7501 gccaggaaga ttcatcaatc actgcctggg tattatctgg caatacgcgc ggaatgtttt
     7561 cccagaaacc gccgccggtc aggtgcgcaa tggcatgcac atcgaccttt tcaatcaatt
     7621 tcagcactga cttcacgtaa atgcgggtcg gtgccagcag atgatcggct aatggcttac
     7681 catcaagttc ggtggtttgc ggatcacaac cgctgacttc aagaattttg cgcaccagcg
     7741 aatagccatt cgagtgcggg ccgctagagc caagtgcaat cagcacatcg ccgtcgctga
     7801 ctttagatcc gtcgatgatt tctgattttt ctaccacgcc aacgcagaaa ccagcgacat
     7861 cgtaatcttc accgtgatac atccccggca tttccgccgt ttcgccaccc accagtgaac
     7921 agcccgattg cagacaacct tccgcaatgc cgctgatcac cgctgaagcg gtatcaacat
     7981 ccagttttcc ggttgcgtaa tagtcgagga aaaacagcgg ttccgcacct tgcaccacca
     8041 ggtcattaac gcacatggcg accagatcaa taccaatggt gtcgtgacgt tttaagtcca
     8101 tcgccagacg cagcttggta cctacgccgt cagtgccgga aaccagcaca ggttcacgat
     8161 atttttgcgg caatgcacac agtgcaccga agccgcccag accgcccatc acttccggac
     8221 gacgcgtttt cttcactacg cctttgattc ttccaaccag agcattaccc gcgtcaatat
     8281 caacaccggc atctttgtag ctaagagagg ttttatcggt cactgcttgg gtccccacgc
     8341 gttacttgcg gtagaaaaat aaaattcggc gcaattctaa cagggaaagc aaacgtttgc
     8401 gagactgctt tacacaacct ttttgcacgt cttttcccca ggcgcgcggc gaaagaatac
     8461 ttgtaccagg gcaaaggatc gttttcggat ggaataatct tctttcataa ccatttgaat
     8521 ataaaataac tttatctcaa accgttatca ttttgactaa agtcaacgaa aataatattg
     8581 ccgccttgaa gaaaggaggt ataatccgtc gatttttttg tggctgcccc tcaaaggaga
     8641 aagagtatga agatcgtgga agtcaaacac ccactcgtca aacacaagct gggactgatg
     8701 cgtgagcaag atatcagcac caagcgcttt cgcgaactcg cttccgaagt gggtagcctg
     8761 ctgacttacg aagcgaccgc cgacctcgaa acggaaaaag tcactatcga aggctggaac
     8821 ggcccggtag aaatcgacca gatcaaaggt aagaaaatta ccgttgtgcc aattctgcgt
     8881 gcgggtcttg ggatgatgga cggtgtgctg gaaaacgttc cgagtgcgcg catcagtgtc
     8941 gtcggtatgt accgtaatga agaaacgctg gagccggtac cgtacttcca gaaactggtt
     9001 tctaacatcg atgagcgtat ggcgctgatc gttgacccaa tgctggcaac cggtggttcc
     9061 gttatcgcga ccatcgacct gctgaaaaaa gcgggctgca gcagcatcaa agttctggtg
     9121 ctggtagctg caccagaagg tatcgctgcg ctggaaaaag cgcacccgga cgtcgaactg
     9181 tatactgcat cgattgacca gggactgaac gagcacggat acattattcc gggcctcggc
     9241 gatgccggtg acaaaatctt tggtacgaaa taaagaataa aaataattaa agccgacttt
     9301 aagagtcggc ttttttttga gtaaagcgcc tataacacat aatacagagg ataatactat
     9361 gacgcgccgt gctatcgggg tgagtgaaag accgccactt ttacagacaa tcccgcttag
     9421 tttgcaacat ttgttcgcca tgtttggtgc aaccgtcctg gtgcccgtct tatttcatat
     9481 taacccggcg actgtactgt tatttaacgg tataggaaca ctgctgtatc tcttcatctg
     9541 taaagggaaa attccggctt atcttggttc cagctttgcc tttatttcac cggtattgtt
     9601 actgttgccg ttggggtatg aagtcgcgct gggcggcttt attatgtgcg gcgtgctgtt
     9661 ctgcctggtt tcttttatcg tgaagaaagc ggggaccggc tggctggacg tgctgtttcc
     9721 acctgcggca atgggcgcaa tcgttgccgt catcggtctg gagctggcgg gcgtagctgc
     9781 cggtatggcg ggtttactcc cggctgaagg gcaaacgcca gactccaaaa ccatcatcat
     9841 ctctattacc accctggcgg tcacggtttt aggttccgtg ctgtttcgtg gtttcctggc
     9901 aattatcccg attttaattg gcgtgctggt ggggtacgcg ctctctttcg caatgggaat
     9961 tgtcgatacc acgccgatta ttaatgctca ctggtttgcg ctgccgaccc tctatacgcc
    10021 gcgcttcgag tggtttgcca ttctgactat tctgccagcg gcgttagtgg ttattgccga
    10081 acacgtaggg cacctggtcg taacggctaa tatcgtcaaa aaagatctgc tgcgcgatcc
    10141 aggtctgcac cgttcgatgt ttgctaacgg cttgtcgacc gtgatctccg gtttctttgg
    10201 ttccacacca aacaccactt acggagaaaa cattggcgtg atggcgatca cccgtgttta
    10261 cagtacctgg gttatcggcg gtgcggcgat tttcgctatc ctgctttcct gcgtcggtaa
    10321 actggctgct gctatccaga tgattccatt gccggtgatg ggcggcgttt cgctgctgct
    10381 ttacggggtc atcggtgctt ccggtattcg tgtgttgatc gaatcgaaag tggattacaa
    10441 caaagcacag aacctgatcc tgacttccgt tattttgatc atcggcgtca gtggtgcgaa
    10501 ggtaaacatt ggcgcggcgg agctgaaagg tatggcgctg gcgaccatcg tcggtatcgg
    10561 cttaagtttg atcttcaaac tgattagtat gctgcggcct gaagaagtgg tgctggacgc
    10621 agaagacgcg gatataacag acaaataatg taccgaccgg gcagtgttgc tgcccggttc
    10681 aaacatcatg ggattctgtg gtaaacttct cgcgattttg tgaaatcctg gttgaggtat
    10741 ctctgaacac accggcacag ctctctttgc cactttatct tcctgacgac gaaacctttg
    10801 caagtttctg gccgggggat aactcctctt tactggccgc gctgcaaaac gtgctgcgtc
    10861 aggaacatag cggttacatc tatctctggg cacgcgaagg cgcggggcgc agccatctgc
    10921 tgcacgcggc ttgcgcggaa ttgtcacagc gtggcgatgc ggtgggctat gtcccgctgg
    10981 ataaacgcac ctggtttgtt ccggaagtgc tcgacggtat ggagcatttg tcgctggtct
    11041 gtattgacaa cattgagtgt attgccggcg atgagttgtg ggagatggcg attttcgatc
    11101 tctacaatcg aattctggaa tcgggcaaaa cacggttgtt gatcaccggc gatcgtccac
    11161 cgcggcagtt gaatctggga ttaccggatc tcgcgtcgcg tctcgactgg gggcagatct
    11221 acaaattgca gccactttct gatgaagata agttgcaggc gctacagtta cgcgcgcgtt
    11281 tgcgtggttt tgaactgccg gaagatgtgg ggcgtttctt gctgaagcgg ctcgacagag
    11341 aaatgcgcac gttgtttatg acgctggatc agctggatcg tgcgtcgatt accgcgcaac
    11401 gcaagctgac cattcctttt gtgaaagaaa ttctgaagtt gtagataagg tgtttattgt
    11461 cggatgcggc gcgaacgcct tatccgacct acgcgaagtt aatccatagt attcgtaggc
    11521 cggataagac gcggcaagcg tcgcatccgg caatgtgctt caactagcga tggagcacat
    11581 cttaacccac gatttccagt acctgctccg gtggacgacc aatccgcgct ttgccattcg
    11641 cgaccacaat cgggcgttcc attagcttcg gattctctac catcgcctga atcagcacct
    11701 cttcgctaag cgaactgtca gccaggttca gttctttata aagatcctct ttttggcgca
    11761 tcaattcacg agcgctattc atccccagca ttttcagcaa atcacgcagc gttgccgcat
    11821 cggcgggtgt ctcaaggtag agcaccactt ctggctccac gccgttttct ttcagcaggt
    11881 tcagcgtttc ccggctcttt gaacagcgtg ggttatggta gatttttgcc tgtttggtca
    11941 ttgttcttcc tttaatgcga attacatctt ggtataaggc ttaaagcgtt cctgcagctg
    12001 gcgcaactgg tcgatgcgcg catcgtaacg cgcttgttgc aggctgccta atttcacctg
    12061 cgaactggcg ctactcaaca aggaaatagc ctgatcgagt cgcccggcga gcgcataacc
    12121 ttctgctcgc gcagccagtt cctgatcgtg gttatttagt gcggcttccg cctgtgccag
    12181 taaatcccag ccgttgctgt catctttatt gttaaaagtg tagcgattca gaatattggc
    12241 cgcttcctgt ggttgccccc cttgcagata agcgttcgcc aggttgagct gcaacaccgg
    12301 attggtgcgc aaatcgcggg catttttcag gcgattgata gcgtcattgg ctttgttttg
    12361 cccaagatcg atatcggtag ccagatcgag ataccatgcg ttgccaggtt ctgccgccag
    12421 taacggttgc agcgttttac gcgcctcgtc gtatttattg gcttccatcg cctgtaaagc
    12481 acgaccatat tgcgccgctc gttgctgacg aacgtttcct ttcgcccatt catccagcaa
    12541 atcactggtg agctggttac gtccggaatt atacatcccc agtgtgcgcg ctttcgccag
    12601 atagaaatct tccgacgact gcaccaccat cgggcgcatc tgattagcac ggttgcgggc
    12661 atctgccaga cgactttccg gcaacgggtg agtcaataaa atttccggcg gacgcgaaga
    12721 gtaacgcgcc tgatcgagta atttttcgag gaaggtcggc atcgcctgcg gatcgaatcc
    12781 cgagcgttgc agcacctgaa taccaatgcg gtccgcttcc tgttcatttt gctgggtgaa
    12841 actgatcatc ccctggcgcg ttcccgccag tgtaccggtc agcgccgcca tccccgcctg
    12901 cggactggcc atcgccagta aaatagaacc taacgcgccg acccaggtca gcggcgcgct
    12961 gcgctgctga tcttccatcg ctcgcgccag gtgacgttgg gtgacgtggg agatttcgtg
    13021 cgccataact gaagccagtt gactttcgtt atcggaataa cggaacaggg cagagtgcag
    13081 caccacgttg ccgccaaaga aagcaaaggc gttaatttcg tcgttgttga tcagaaagaa
    13141 atgaaacggt gtcttaaccg aattggcatg cgaaaccaga cgcattccta gcgaattaat
    13201 gtattgcgtt aacagcggat cattaattaa cggcgcgctg ccgcgtaact ggcggacata
    13261 atagtcgccc atctgcattt cctgaccaat ggaaagtgtg cttcctgcgg aggttcccat
    13321 atccggcaag gtgtctgcgc tgtcggcaaa cgccggggct acctgaccaa tggtcatagc
    13381 agcaatgagg gttgcaacca ggtttttttt caactgcctg aacataacct ctatcctgta
    13441 tttctggaga tagtcatttg accgatactg tgaaatatcg ttcccaccgc gccgcctttg
    13501 agtgtaacgg cgaagattag cccactcaaa tgacttttca gtttcctgaa tccgtcataa
    13561 aaagcgaagt cttttttgtg acatttcgat acaattgcgg atcgcaatcc cgctcttgag
    13621 gttcagggaa ggtctgtatg ctcgaaatgt tgatgcaatg gtatcgccgc cgttttagcg
    13681 acccggaagc gattgccttg ctggttattt tggttgctgg ttttggcatt atctttttct
    13741 ttagcggcct actcgcgccg ttgctggtgg ctattgtgct ggcctattta ctggaatggc
    13801 cgactgtccg cctgcagtct attggctgtt cccgcccttg ggcgacatct atcgtgctga
    13861 tcctgtttgt gggcatcctg ctactgatgg cgttcgtggt actgcctatc gcctggcagc
    13921 agggcatcta cttaatccgc gatatgccgg ggatgctcaa taagctttct gactttgccg
    13981 ccacgttgcc gcgccgctat ccggctttaa tggatgcggg cattattgat gcaatggctg
    14041 aaaatatgcg cagtaggatg ctgaccatgg gcgattcggt ggtgaaaatt tccctcgcct
    14101 cgttggtcgg tttgctgacc attgccgtct atctggtgct ggtaccattg atggtcttct
    14161 tcctgctgaa agacaaagag cagatgctga acgccgttcg ccgggtgctg ccgcgcaacc
    14221 gtggactggc aggacaggtg tggaaggaga tgaatcaaca aatcaccaac tatatccgcg
    14281 gcaaagtgct ggagatgatc gtggtaggga tcgccacctg gctggggttc ttgctctttg
    14341 ggctgaacta ttcgctgctg ctggcggtgc tggtcggctt ctcggtactt attccgtaca
    14401 ttggcgcatt tgtggtgacc attccggtgg ttggcgtggc gctattccag tttggtgctg
    14461 gcacggaatt ctggagctgc ttcgcggtgt atctgattat tcaggcgctg gacggcaacc
    14521 tgttagtacc ggtgttgttc tccgaagcgg ttaacctgca tccgctggtg attattttat
    14581 cggtggtgat cttcggtggt ttgtggggat tctggggcgt attcttcgct attccattgg
    14641 cgacgctgat caaagccgtg attcacgcct ggcctgatgg gcaaatcgcg caagactgac
    14701 cttcagcgaa aaaatttgaa acgagtgttg cagggcagtg agaagatgtg agatgtgctg
    14761 gccgcaagcg cagccagcac ggaatggagc aaagtaatca ggcgtgttct ttcagccagt
    14821 tcagcacaac gtcgtggtga ttgctggttt tgaaatcgtc aaagacatgt tcgattttgc
    14881 catcagcgtc aatcaggaag ctgatgcgat gaataccatc gtaggttttg cccatgaagg
    14941 acttttcacc ccagacgccg aattgttcgc acacctggtg gtcctcatca gacaggagcg
    15001 taaagttaag cagctctttt tccgcaaaac gggagagttt ttcgggttta tcggtgctga
    15061 tacccagcac atcaacgccc gcttttttca actcatccat gttatcgcgt aatccgcagg
    15121 cctgtacggt acagccgggg gtcatggctt tcgggtagaa ataaaccaga acacgctgtc
    15181 cctggaagtc ggtcaaatta acttgttctc cgtcttgatc cggcaagcta aatttcggtg
    15241 cgatatcacc ggctttcagt ggattcatta cttaactcca tcctgttcat catgttggga
    15301 ataattgacg acgttaatac tgccttgtgc attgagttct gtacataggg ctttgaacgc
    15361 ttgctcaata tttgctgcgt ccgcagatgc ggggctgtgg gcagttatct gaatatgcaa
    15421 ctgcgcagcc ctttcatttt cagcaggttg cgtgcgcgac accagctccg caatgttcat
    15481 atgatgcgtg tcgaaaagtg ctgtgaagcg ttcaattaaa tgcggggagt ctgccacatc
    15541 gacctgaacc cagacagatg ctggcattgg cggacgcgga cgcgccgtcg tgcgcttcat
    15601 cacgattaaa agatccagct cggcaccttt caacggtaac gttgattcaa tcagagtaat
    15661 ggcattccat gaaccggaaa gcagcatgat aaacgtgaac tcttctccca gcatcgccag
    15721 gcgactgtct tcaatattac agccgcaact actgacatga cgggtgatgg tgttcactat
    15781 tccagggcga tcggcaccca acgcagtgat caccagataa tgttgcgatg acagtgtcaa
    15841 actggttatt ccttcaaggg gtgagttgtt cttaaggaaa gcataaaaaa aacatgcata
    15901 caacaatcag aacggttctg tctgcttgct tttaatgcca taccaaacgt accattgaga
    15961 cacttgtttg cacagaggat ggcccatgtt cacgggaagt attgtcgcga ttgttactcc
    16021 gatggatgaa aaaggtaatg tctgtcgggc tagcttgaaa aaactgattg attatcatgt
    16081 cgccagcggt acttcggcga tcgtttctgt tggcaccact ggcgagtccg ctaccttaaa
    16141 tcatgacgaa catgctgatg tggtgatgat gacgctggag ctggctgacg ggcgcattcc
    16201 ggtgattgcc gggaccggtg ctaacgctac tgcggaagcc attagcctga cgcagcgctt
    16261 caatgacagc ggtatcgtcg gctgcctgac ggtaacccct tactacaatc gtccgtcgca
    16321 agaaggtttg tatcagcatt tcaaagccat cgctgaacat actgacctgc cgcaaattct
    16381 gtataatgtg ccgtcccgta ctggctgcga tctgctcccg gaaacggtgg gccgtctggc
    16441 gaaagtaaaa aatattatcg gaatcaaaga ggcaacaggg aacttaacgc gtgtaaacca
    16501 gatcaaagag ctggtttcag atgattttgt tctgctgagc ggcgatgatg cgagcgcgct
    16561 ggacttcatg caattaggcg gtcatggggt tatttccgtt acggctaacg tcgcagcgcg
    16621 tgatatggcc cagatgtgca aactggcagc agaagggcat tttgccgagg cacgcgttat
    16681 taatcagcgt ctgatgccat tacacaacaa actatttgtc gaacccaatc caatcccggt
    16741 gaaatgggca tgtaaggaac tgggtcttgt ggcgaccgat acgctgcgcc tgccaatgac
    16801 accaatcacc gacagtggtc gtgagacggt cagagcggcg cttaagcatg ccggtttgct
    16861 gtaaagttta gggagatttg atggcttact ctgttcaaaa gtcgcgcctg gcaaaggttg
    16921 cgggtgtttc gcttgtttta ttactcgctg cctgtagttc tgactcacgc tataagcgtc
    16981 aggtcagtgg tgatgaagcc tacctggaag cggcaccgct tgcggagctt catgccccgg
    17041 ctggaatgat cttgccggtg acctccggcg attatgcaat cccggtgacc aacggtagtg
    17101 gtgccgtcgg taaggcgctg gacattcgtc caccagccca gccgctggca ctggtttctg
    17161 gcgcgcgtac ccagttcacg ggcgataccg cttcattgct ggtggaaaat ggtcgtggca
    17221 atactctgtg gccgcaggtg gttagcgtgc tgcaggcgaa aaactacacc atcacccaac
    17281 gtgatgatgc tggtcagaca ctgaccaccg attgggtaca atggaaccgt ctggacgaag
    17341 acgagcagta tcgtggtcgt tatcaaatct ctgttaagcc gcagggttat cagcaggcgg
    17401 ttacggttaa actgctgaac ctggaacagg cgggcaaacc ggttgcagac gcggcttcca
    17461 tgcagcgtta cagcacggag atgatgaacg ttatttccgc cggtctggat aaatctgcca
    17521 ctgacgccgc gaacgctgcg caaaatcgtg cctccaccac tatggacgta caaagtgcag
    17581 ctgatgacac cggtttacca atgctggtcg tacgcgggcc gttcaatgtg gtttggcaac
    17641 gtctgccagc tgcgctggaa aaagtgggca tgaaagtgac cgacagcacc cgttcgcagg
    17701 gcaacatggc cgtaacttat aagccgctgt ctgacagcga atggcacgaa ctgggcgcga
    17761 gcgatccagg tctggcatcc ggtgactata aactgcaggt tggcgattta gataaccgca
    17821 gcagcctaca gttcatcgat ccgaaaggtc atactctgac tcagagtcag aacgacgcgc
    17881 tggtagctgt cttccaggct gcgtttagca agtaaaaata cagggctgga gtcatccggc
    17941 ccttttttct gatatgatac gcaaacgtgt gcgtcgacag aaaaacgcga ttttagcggt
    18001 aattcgcacg aaatttgttt gtcggacgta ggtcggataa ggcgtttacg ccgcatccga
    18061 caaaacatcc ggcacaccag acagcaaaag attttaaaac gttaattcac acccaggagt
    18121 gataaagatg caaaagcaag ctgagttgta tcgtggtaaa gcgaaaaccg tatacagcac
    18181 ggaaaacccg gacctgttgg tgctcgaatt ccgcaatgat acgtcagcag gggatggcgc
    18241 gcgcattgag cagtttgatc gcaaaggtat ggtgaacaac aagttcaact acttcattat
    18301 gagcaaactg gctgaagcgg gtatcccgac tcaaatggag cgtctgctct ccgataccga
    18361 atgtctggtg aaaaagctgg atatggtgcc ggttgagtgt gtcgtgcgta accgtgctgc
    18421 tggctctctg gtgaaacgtc ttggaatcga agaaggtatt gagctgaacc cgccactgtt
    18481 cgatctgttc ctgaaaaacg acgccatgca cgatccgatg gtcaacgaat cttactgcga
    18541 aacctttggc tgggtgagca aagagaacct ggcgcgtatg aaagagctga cctacaaagc
    18601 gaacgacgtg ctgaaaaaac agttcgatga tgctggtctc attctggtcg acttcaagct
    18661 ggagtttggc ctgtacaaag gtgaagtggt actgggcgat gagttctccc cggacggcag
    18721 ccgcctgtgg gacaaagaaa cgctggagaa aatggataaa gaccgtttcc gccagagcct
    18781 cggtggcctg atcgaagcct atgaagccgt tgcccgccgc ctgggtgtac agctggactg
    18841 atttttctgt tcatcatctt gccgtgctgc tggcacggca agacaaccgc tctcataaga
    18901 tgtgcattga acgctcattc ctccgccatt tcatcccgct ttcctgtggt aatcttcctc
    18961 tgaaccacct acgatcattt ctataatgaa tataatgttg aggtatctat gcgttggcaa
    19021 ggacgacgtg aaagtgacaa tgttgaagac aggcgcaata gctccggcgg tccgtcaatg
    19081 ggcggtcccg gttttcgcct gccaagcggt aaaggcgggc tgattttact gatagtcgtg
    19141 ctggttgcag gctactatgg cgttgattta accgggttga tgaccggaca gccggtttcc
    19201 caacagcaat caacgcggtc aattagccct aatgaagacg aagccgcaaa attcacctcg
    19261 gtgattctgg caaccacgga agacacctgg ggacagcaat tcgagaagat gggtaaaacc
    19321 tatcagcaac cgaagctggt catgtaccgt ggaatgacgc gtaccggctg cggggcgggc
    19381 cagtccataa tggggccatt ctattgcccg gcggatggca cagtttatat cgatctctcc
    19441 ttctatgatg acatgaaaga caaacttggc gcggatggcg attttgccca ggggtacgtt
    19501 atcgctcatg aagtcggtca tcatgtgcag aaactgttag gcatcgagcc gaaagttcgt
    19561 caactgcaac aaaacgcgac gcaggcggaa gtaaaccgct tatctgtgcg tatggaactc
    19621 caggccgact gttttgccgg tgtctggggg catagtatgc agcagcaagg tgttctggaa
    19681 accggcgatc tggaagaggc gctgaacgcg gcgcaggcca tcggcgatga ccgtttacaa
    19741 cagcaaagtc aggggcgagt agtaccagac agtttcactc atggcacttc tcagcaacgc
    19801 tacagctggt ttaaacgtgg tttcgacagc ggcgatccgg cacaatgcaa tacttttggt
    19861 aaaagcattt aacttccggg gcagggatgg ctgaactgac tgcgcttcac acattaacag
    19921 cgcaaatgaa acgtgaaggg atccgccgct tgctggtgtt gagcggggaa gagcgttggt
    19981 gttttgatca tgcgcttaag ttgcgtgatg ccttacctgg cgactggctg tggatttcgc
    20041 cgcagccaga tgctgaaaac cactgttctc cctcggcact acaaacttta cttgggcgcg
    20101 agttccggca tgcggtattc gacgcccgcc aaggctttga tgccgctgcc tttgcggcac
    20161 ttagcggaac gttgaaagcg ggaagctggc tggtgttgtt actccctgta tgggatgagt
    20221 gggaaaacca acctgatgcc gactcgctgc gctggagtga ttgccctgac cctattgcga
    20281 cgccgcattt tgtccagcat ttcaaacgcg tacttacggc gaataacgac gctatcctct
    20341 ggcggcaaaa ccagccgttc tcgttggcgc attttactcc ccgtactgac tggcaccccg
    20401 ctaccggcgc accacaacca gaacaacagc aactcttaca gcagctactg accatgccgc
    20461 cgggcgtggc agcggtaact gctgcgcgtg ggcgcggtaa gtcggccttg gcagggcaac
    20521 tcatttctcg tattgcgggc agtgcgattg tcaccgcgcc cgcaaaagcg gcaacggatg
    20581 tactggcaca atttgcgggc gagaagtttc gctttattgc accggatgcc ttgttagcca
    20641 gcgatgagca agccgattgg ctggtggtcg atgaagccgc agctatacct gcgccgttgt
    20701 tgcatcaact ggtatcgcgt tttcctcgaa cgttgttaac cactacggtg cagggctacg
    20761 aaggcaccgg acgtggtttt ttgctgaaat tttgcgctcg ctttccgcat ttacaccgtt
    20821 ttgaactaca acagccgatc cgttgggcgc agggatgccc gctggaaaaa atggttagtg
    20881 aggcactggt gtttgacgat gaaaacttca cccatacacc acaaggcaat atcgttattt
    20941 ccgcatttga acagacgtta tggcgaagcg agccagaaac gccgttaaag gtttatcaat
    21001 tattgtctgg tgcgcactac cggacttcgc cgctggattt acgccgcatg atggatgcac
    21061 caggtcaaca ttttttacag gcggctggcg aaaacgagat tgccggagcg ctgtgtctgg
    21121 tggatgaggg tggattatct caagaactca gtcaggcggt atgggcgggt tatcgtcgtc
    21181 cacggggcaa tctggtggct caatcgctgg cggcgcacgg cagcaatcca ctggcggcga
    21241 cattgcgcgg acggcgggtt agccggatag cagttcatcc ggctcgtcag cgggaaggca
    21301 cagggcgaca gcttattgct ggtgctttgc aatatattca tgacctcgac tatctttcgg
    21361 tgagttttgg ttacaccgag gagttatggc gtttctggca acgctgcggt tttgtgctgg
    21421 tgcgaatggg taatcatcgt gaagccagca gcggttgcta tacggcgatg gcgctgttac
    21481 cgatgagtga tgcgggtaaa caactggctg aacgtgagca ttaccgttta cgtcgcgatg
    21541 cgcaagctct cgcgcagtgg aatggcgaaa tgcttcctgt tgatccacta aacgatgccg
    21601 tcctttctga cgacgactgg cttgaactgg ccggttttgc tttcactcat cgtccgctat
    21661 taacatcgtt aggttgctta ttgcgtttgc tacaaaccag cgaactggca ttaccggcgc
    21721 tgcgtgggcg tttacagaaa aacgccagtg acgcgcagtt atgtaccaca cttaaacttt
    21781 caggccgtaa gctgttactg gtccgtcaac gcgaagaagc cgcgcaggcg ctattcgcac
    21841 ttgatgatgt ttgcactgag cgtctgcgcg atcgcataac gcaatggcaa ttttttcact
    21901 gactccttca attttctccc atggtcattg tgagtcagcg gcgtaaaatt aatgacatca
    21961 gttaaggagt tcgccatgaa acatgaccat tttgttgttc aaagcccgga taaaccagca
    22021 caacagttgt tactgctttt ccacggtgtc ggggataacc cggtggcaat gggggaaata
    22081 ggtagctggt ttgcgccgct gtttcctgat gcgctggtag tgagtgtcgg tggcgcggag
    22141 ccgagcggta atccggcggg gcgtcagtgg ttttcggtgc agggtattac ggaagataat
    22201 cgccaggcgc gtgtggatgc gatcatgccg acgtttattg agacggtgcg ctactggcag
    22261 aaacaaagcg gagtaggggc taatgccacg gcgctaatcg gtttctcaca aggcgcgatt
    22321 atggcgctgg agagcattaa agccgaaccg ggccttgctt cgcgcgtcat tgccttcaac
    22381 ggacgctacg cgagcctgcc ggaaacagcg tctacagcca caacaattca cttgattcat
    22441 ggcggtgaag atccggttat cgatctggcg catgcggttg cggcgcagga agcgttaatc
    22501 agtgccggtg gcgatgtgac gctggatatc gtggaggatt taggccatgc gatcgacaat
    22561 cgcagtatgc aattagccct ggatcacttg cgttatacca ttccgaaaca ttatttcgac
    22621 gaagcgctaa gtggcggtaa gcctggcgat gatgatgtga ttgaaatgat gtaataatag
    22681 ccgggtaggc ctgataagac cgtatcaggc atcatggccg gatgcggcgt taacgcctta
    22741 tccggccact tagcttactt cttcggttga tcctttttcg gccagtcgtc gtcatcgtcc
    22801 catttatcgt tgaaatcacg atgcggggga agctccggct tgttggcgag aaatttctta
    22861 tggtcgacgc gcttgagatc tttaatcacg ttcagcagga cgcctactaa aaagacaatc
    22921 accagaatcc accaatattt agccagccag tccatgctta tttcctcttg cagaaccact
    22981 catcaggcga cgagctgttc catgatacgt tgatacatac gggcaagtag ctgcaggtcg
    23041 gcagcgttca cacattcatt aattttatga atcgtggcat tgaccggccc gagttccacc
    23101 acctgcgccc ccatgcgggc aataaagcgc ccgtcggacg ttccgcctgt ggtcagtagc
    23161 tgcggtttaa tttcattata gtgctcaacc gcgttaacga ccgcatccac cagtttaccg
    23221 cgcgcggtca aaaatggctg cccggaaagc caccaatcca ccgtatagcg cagttgatgt
    23281 ttttcaagca gggcaagtac ctgcgctttg atcatctcat cagtcagttc ggtgctgaag
    23341 cggaagttaa actgcacaaa cagttcaccc ggaataacgt tgttactgcc cgttcccgcc
    23401 tgaatattgg caatctgcat actggtcgcc gggaagaatt cattgccctg atcccactca
    23461 atagccacta attcattaag gaaaggtgct gcgcgatgta ccggattgtc agccagatgt
    23521 gggtaggcaa catgcccctg cacgccatga atagtaaggt tacaggttaa cgatccgcga
    23581 cgaccatttt tcaccacatc accaaccact tcgatactcg acggttcgcc gaccaggcag
    23641 taatcgagac gctcattacg cgccattaac gcttcgacga cttttaccgt accgttgtgg
    23701 gcactggctt cttcatcaga ggtgatcaga aatgccagtc gtcccgtatg gttgggatgt
    23761 tgtgcgacaa agcgttctgc cgctaccacc atcgccgcca gcgagccttt catatctgcc
    23821 gcaccgcgcc cgaataacat gccatcacga atggtgggtt caaacggcgg attgatccaa
    23881 cggtcggcgt cgccaggcgg caccacgtcg gtatgcccgg caaaggctaa cgtttcaccc
    23941 tgcccacgcc atgcccaaaa attctgcgtg tcggcaaagt ccatgcgttc aacggtaaaa
    24001 ccgatcgcct gcaaacgttc aatcaacaaa gcctggcatc ctgcgtcatc aggactcagg
    24061 gaagggcggc gaataagctg ttgtgtcagc tcaataaccg ggcacgacat agactacacc
    24121 tcatggaaaa attgctgata actggattca ctgaaaccca gcagcatagg cttaccaggc
    24181 gcgcagagca atggacgttt gataattgca ggcatttcag tcattaatgt cgccgcagag
    24241 gccgcatcgg tgattttatt gcgggtggtt tcgtccagtt tacgccaggt tgtaccacgg
    24301 gtgttgagta acgcttccca gcctaattcg ttgataaaac cgttcaataa ttcgctgtcc
    24361 aatccatcga cgcggtaatc atgaaaacga tagtcgatgt tattagcttc cagccaacgg
    24421 cgagccttct taatggtgtc acaatttttg atgccgtaaa gtgtaaccat aatgaatcct
    24481 ttttcgattt atgagaataa atactcattt aaaggtgaaa acattttgcc tgcaaatgat
    24541 tatcaggaaa tataacatta tcgaagtatt caaattaaca accattaaat aacattaaac
    24601 atttttaacg taaacattaa gaatattcat cttgttatct gtctgtattt taaccagaat
    24661 taaatcagta ttagatgccg tatgaatatc tcgaaatgtt gcggtgcatc acatacaaat
    24721 catcaccgca ggaccatagt atttacataa ggtttgtccc ccggaggtag tcatcacagc
    24781 aattgagtaa attagcttca ccacggtata ttgtttccgt agaataccca taattataag
    24841 agaggttgtt atgattgaac gtgaactggg gaactggaaa gactttatcg aagttatgct
    24901 tcgtaagtaa ttcctgagac aaagtacaaa cagcaagaat ccgctgagca ggttcttaat
    24961 cggaaagttg aaaagggcga cacattggca tgtcgccttt tttattgctt attccgggcg
    25021 cggcttcagc gggaagcggc ggcgcaccag cacaaagaac agcggcacga agtaaatagc
    25081 cagaatagtg gccgaaatca tcccgcccat tacgccagta cccaccgcat gctgaccacc
    25141 ggaaccggca cctgtgctgg ttgccattgg caatacgccg aagataaatg ccagcgaggt
    25201 catcagaatc gggcgtaaac gctgacggca ggcgtggagc gtcgcttcaa acaggtcgtg
    25261 gcctttttgg ttcatctcgt tagcaaactc gacgatcagg atagcgtttt tcgccgataa
    25321 accaatgacc gttaacaggc ccacctggaa gtaaacatcg ttttccagcc cgcgcatcca
    25381 ggttgccagc agcgcgccga taacccccag cggcacgacc agcattaccg agaacggcac
    25441 cgaccagctt tcatacagcg cagccagaca caggaatacc accagcaagg aaatggcgta
    25501 cagcgccgga gcctgcgcgc cggaaagccg ctcctgatac gacatcgccg tccactccag
    25561 accaaagccg tttggcaact gcttcactaa cgattccata atatccatcg ccgtaccggt
    25621 actgaccccc ggtgcggctt ccccaacaat ctcaaccgca gaataaccgt tatagcgttc
    25681 cagacgcggc gagcctgtct cccagcgtga ggtcgcgaaa gcagagaagg gcaccatgcc
    25741 gccatcttta tttcggacat accagagatt gatgtcatct ggcagcatgc gatacggcgc
    25801 agctgcctgc acatagactt tcttcacgcg accgcgatcc cacatagctc gaaccccagg
    25861 cggtttgcag tgtgtcgtta atatcgtcga tagcaacgcc cagcgcctga gctttacgct
    25921 ggtcgatatc aatctgcaac tgcggactgt cgtcgaggcc gttatggcgc acacgggtta
    25981 gctccgggtt ttccgctgcc agcgccagca actggtttcg tgctgccatc agcgcatcgt
    26041 gacccgctcc agcgtggtcc tgcaactcca tatcaaaacc cgcagaacta ccaagaccgc
    26101 taattgctgg cgggctgctg gcgataacgc gagcttcttt aatcttgtta aacgccttcg
    26161 ttgcacgctc gataatggca aacgaggtgc cggtcttact gtcgcgttcg ctccagtctt
    26221 tcaggcggat aaacattcgc gccacgtttt gcccgttacc cccagggcca gaacccacgg
    26281 tggcaaacac cgacatgatg ttgtcttttt catgggtgaa gtaatatttc tcgatttgct
    26341 caacgacttt cagggtctgt tgctgggttg aaccgctggg caactgtacc gaggtagtaa
    26401 acatgccacg gtcttccagc ggtaagaacg acgtcggcaa acgcaggaac aggaacacca
    26461 tgccgccaag cagcaggaca taaatcacaa tccagcgcag gctacggtgg agaattttcg
    26521 ccaccccttt ttcgtagcgt tcggagttgc ggttaaacat ctggttaaac caggcaaaaa
    26581 agcctttttg cccgtgatgc tcaccttttt tcagtggctt aagtagcgtg gcacacagag
    26641 caggagtgag gatcatcgct accagtactg acagcaccat cgccgcaaca atggtaatag
    26701 agaactggcg atagatggca ccggtggtgc cgccgaagaa ggccattggc acaaataccg
    26761 ccgaaagcac catcgcaatc ccgaccagtg ccccctggat ctgccccatc gatttacgtg
    26821 tggcttcgcg aggagtgagt ccttcctcac tcataatacg ttcgacattt tccaccacca
    26881 cgatggcatc atccaccagc agaccgatcg ccagcaccat cgcgaacatg gttaaggtgt
    26941 tgacactgta accgaaggcg taaagcacgg agaaggttcc catcaacacc accggcacgg
    27001 cgatagtggg tatcagcgtg gcgcggaagt tttgcaggaa cagatacata acgaggaaaa
    27061 ccagggcgat agcttccaga agcgttttca ccacgtcttc aatggaggct ttaacaaacg
    27121 aggtggtttc atacgccacc ttgtattcca gtccgtgtgg aaaatactgc gccagctcgt
    27181 cgagacgatt gagcaccagc tccgctgtcg ccatttcgtt cgcgccggag gccagtttaa
    27241 cccccagccc ggaggctggc ttaccattga agcggctaag ataatcgtat ttctccgccc
    27301 ccatttcgac ggtggcgaca tcgcccagcc ttacctctga gccgtcctga ttcacccgca
    27361 aggtgatatc gcggaactgt tctggtgttt gcagcagtga ctgggcgtta atggtggcgt
    27421 tgagcgcctg cttatcgacg gaaggcgtac caccaagttg cccaaccgca atctgcgcgt
    27481 tctgtgattc aatggcatcg gtgacatctt tagccgtcat ctggaaactg ttgagtttcg
    27541 ccgggtccag ccagatacgc atggaatatt gcgaaccata ggcatcgata tccccgacgc
    27601 cattcacgcg gcttaacggg tcctgaatat tactggcaac ataatcggca atatcctgtt
    27661 tatccatcga accatcggta gagacgaagg caatggtcag aatgttggta tcgccggttt
    27721 tacgcaccgt cacgccctga ttttgcaccg cctgcggtaa ctttcgcatg gctgattgca
    27781 gctggttttg tacttgctgc acggcttcgt ccggatcggt gcctgcttta aaacttaacg
    27841 tgacagatgc ctgaccggtg ccgctgctct gagatgacat atacatcaga ttatcgaggc
    27901 cggtcatatt ttgctcgata acctgggtca cggtgttttc cagcgtctgg gccgatgcgc
    27961 cgggatagtt agcggtcact cgcacattcg gtggcgcgag atcggggtat tgttcaacgg
    28021 gcaatgaaaa aatcgccagg gtacctgtca gacacaacag gattgccagc acccaggcaa
    28081 aaatggggcg atcaataaag aaattcgcca ttaaaagagg acctcgtgtt tctgcgtatc
    28141 gttgtaggct tggcttgctt cactgtagcg gcaaggtacg agccaatcgt ggagaaaaaa
    28201 aggagttaat gtaaattatc atgccctttt atactgaacc ttaagtgcca ctattctttg
    28261 gtcaataaga ggcattacat cagctgactt tcctcaccct ccgcagagcg aaagctaata
    28321 ctcactaacg tgccgccgcc ggaaggttgt gaaaaagtca gcgtcccacc caatctttcc
    28381 gcgcgttcgc gcatgatatt cagaccataa tggccttcgg gttctttcgg ttcaccgatg
    28441 ccaatcccgt tgtcgcgaat atagactgtg tgattgccat ccggcgcggt gacgcagcta
    28501 acggcgattt cgctggcgtt ggcgtgcttc atcgcattca gcaccgcttc gcgaataatt
    28561 tgcaacaaat gcacctgcat ttgcgcatcc agcgccaggg ttggcagacg gcagtcgagg
    28621 gtcagtttgg cgctggtttg attttgtaac gtatccagca tttccctcaa cgcggagggg
    28681 agatccgcct gctgcagcgt caggcggaaa gtagtcaaca gctcgcgtaa ctgccgataa
    28741 gcatcattca acgcctggga aaaatcggcc atgatacttt gtgcggtggc gttatcttcc
    28801 ggtatcgaac gcttcagtaa cgtcaactgg atacgtaagt aagaaagtac ctgagccagc
    28861 gagtcgtgca attcgcgggc gatggtcgca cgttcttcca tcaacaataa ctgctgaaaa
    28921 tgcttctgcg cctgattaaa gtacaaaccg cgtcccagca tcgacgaaac gctgttaagc
    28981 agcggttctg atgatgaaac gtgactattt tgccagtgca gttcgccgta aaccgtctct
    29041 tgcattgtca ccggtaaaat ctgcatcggc aattccgggt ttggctgccc ttcgctaatc
    29101 cgccagttgt caccgacatt taactccaga tattcagccg cttcattgtc gcgaacaatc
    29161 tgcaaaatat ggcggaaaca atgcacatca atctggctgg tgtttagcgc ctgcgaacac
    29221 tgatacaaca cctccagacg acgcttggcc tcgtggagat cgcgggtctt ttcttctact
    29281 gacgcctcca gcgaacggta caatttatgc agctcgctcg acatctggtt aaaggttttt
    29341 gcaagcaggc caagctcatt cggcaggctg gtatccagcg gcggcgagtc gaactgcccg
    29401 tgttcaatac gctgactggc ggtaaccagc tgattcagcg gggcaaccac ctgatggcgt
    29461 atgcggcgca aagtaaaaaa gaccagcgtg aaaataccga tgccgccagc cagggaaatt
    29521 gccaccacca gcagcatttt gcgttcagcg tagtgctgta aagccagcac gaacaggtct
    29581 atctgattaa cataattatt aatattggcc tgataccacg gcaagtcgcc cttgctgagc
    29641 cgattattca tctccagcca attggcattc agatgcgcat agcgggtttt tactgcctct
    29701 ggcacatacc agacgttgag attggttaat accggtgaat gcagtgcctg ctgaaataac
    29761 tggcgatgtg cattgagttg tggactgcca ctttgcaagt cgtagcccag gcggtaactc
    29821 tgcatacgca gcgatccggc aatattgata gcctcagcgt cgcgtaaact gctcgccagc
    29881 gttagcagag cgatacccgt ggaaagaatc gacagcagca caatgtaaaa aaaggcccgg
    29941 gccagactgg ccgagacggg tcgtttaaca atcacacgcg tcttctccac aaaaaatcat
    30001 taatgttcca gttcgctgaa acgaaattta gtcagtgatg ggattatttg atctgccaca
    30061 gtttatagcg aaatagttac ttattctggg cacatgaaca ttgccgattc ccctgtaagt
    30121 cgggtaataa caacactcat ataaagaata aggtttttac aaccaaaaaa gaaggtcgtt
    30181 atgaatcgtt ttattatggc caacagtcag caatgtctgg gttgtcatgc ttgtgaaatc
    30241 gcctgtgtca tggctcacaa tgacgagcaa catgtcctga gccaacacca ttttcatccc
    30301 cgaattacgg ttatcaaaca tcagcagcaa cgtagtgcag tgacctgtca ccattgtgaa
    30361 gatgcgccct gcgcccgtag ctgccctaat ggcgcaatca gccacgttga tgacagcatt
    30421 caggtcaatc agcaaaagtg tattggctgt aaatcctgcg tggtggcctg tccatttggt
    30481 acgatgcaaa tcgttctgac acccgttgcg gtaggaaaag taaaagccac ggcgcataaa
    30541 tgcgaccttt gtgcagggcg cgaaaacggt cctgcctgtg tcgaaaattg cccggcggac
    30601 gcgctgcaac tggtcactga cgccgcactc tccggcatgg cgaaatcccg ccgcttgcgc
    30661 accgcacgtc aggaacaaca accgtggcat gccagtaccg cggcgcaaga aatgccggta
    30721 atgagtaaag tcgagcaaat gcaggcaacg cccgcgcgtg gcgagccaga taaactggcg
    30781 attgaagcgc gcaaaaccgg ttttgatgaa atttatctgc catttcgtgc cgatcaggca
    30841 caacgggaag cctcgcgctg ccttaagtgc ggcgagcaca gcgtttgtga atggacctgc
    30901 ccgctgcata accatattcc gcagtggatt gaactggtga aagccggaaa catcgacgcc
    30961 gccgtcgagc tttctcacca gaccaacacc ctgccggaaa ttaccggacg cgtttgcccg
    31021 caagaccgtt tgtgtgaagg tgcctgtact attcgcgatg agcacggcgc agtaactatc
    31081 ggcaacattg aacgctacat ttcagatcag gcgttggcga aaggttggcg tcctgactta
    31141 agccatgtca ccaaagtgga caagcgggtg gcgattatcg gtgcgggtcc ggcagggctg
    31201 gcctgtgcag atgttctgac ccgcaatggc atggcggtga cggtgtacga tcgccatcca
    31261 gaaatcggtg gcttgctcac tttcggcatt ccttctttca aactggataa atccctgctg
    31321 gcacgccgtc gggaaatctt cagcgcgatg gggattcact tcgaactcaa ttgtgaagtg
    31381 ggtaaagatg tttctttgaa ttcgcttttg gaacaatacg acgcggtctt cgttggcgta
    31441 ggcacttacc gttccatgaa agcgggttta cccaatgaag atgcgccggg cgtttatgac
    31501 gcgctgccgt tcctcatcgc caacactaaa caggtgatgg gactcgaaga gctaccggaa
    31561 gagccgttta tcaataccgc cggacttaac gtcgtggtac tgggcggcgg cgacaccgcg
    31621 atggactgtg tgcgtaccgc gctgcgccat ggcgcgagta atgtcacctg cgcttatcgt
    31681 cgtgatgaag ctaacatgcc aggctcgaag aaagaggtga agaacgcccg tgaagagggg
    31741 gccaacttcg aatttaacgt ccagccggtg gcgcttgagc tgaatgaaca aggtcacgtc
    31801 tgcgggattc gtttcctgcg cacgcgtctt ggcgagccgg atgcccaggg gcgtcggcgt
    31861 ccagtgccgg tggaaggcag tgaatttgtc atgccagccg atgcggtgat tatggcgttt
    31921 ggcttcaatc cgcacggaat gccgtggctg gagtcgcacg gtgtaacggt agacaaatgg
    31981 ggccgcatca tcgcggatgt ggaaagccag taccgttacc agaccacgaa tccgaaaatc
    32041 ttcgccggtg gtgacgccgt gcgtggtgcg gatctggtgg ttaccgcaat ggcagaagga
    32101 cgtcatgcgg cacaggggat cattgactgg ctgggcgtaa aatcagtcaa atctcactga
    32161 tagcctgcgc agacaaaccc gacttcacag cgtaagataa ttgttcattt cgcgctgtgg
    32221 agtcggtatg acgcaacaaa tcaccctcgt taaagacaaa attctctccg ataactattt
    32281 caccctgcac aacattactt acgatctcac gcgcaaagat ggcgaagtta tccgccataa
    32341 acgtgaagtt tacgatcgcg gtaatggtgc gacgatcctc ctgtacaacg cgaagaaaaa
    32401 gaccgtggtt ctgattcgcc agttccgcgt cgctacctgg gttaatggca atgaaagcgg
    32461 gcaactgatt gaaacctgcg ccgggctgct ggataacgac gaaccggaag tgtgcattcg
    32521 caaagaagcg attgaagaga cgggctatga agttggcgaa gtccgcaaat tatttgaact
    32581 gtatatgtcg ccaggcggtg tgactgagct aatccacttt tttatcgccg aatacagtga
    32641 cagccagcgc gctaacgccg gtggtggtgt cgaagatgaa gatattgaag tgcttgagct
    32701 gccgttcagt caggcgctgg atatgattaa aaccggcgag atacgtgacg gtaagacggt
    32761 gttattgctt aactatttgc aaatgtcaca tttaatggac tgaaaaataa caataatatt
    32821 tcgttgttta ttattggctc aatccgacaa atccgattga gccgtgctac ctgcgcaacg
    32881 aagatacgac ttgtgctgtt tgtttgaact tctggggtcg taccgtccat gcgatatcgc
    32941 attttccttc tctttttttt cgctttgttg ccgatgtctt tggtgtgggc ggcaccagcg
    33001 caacgggcgt tttccgactg gcaggtcacc tgtaataatc aaaatttctg tgtagcgcgc
    33061 aatacgggcg atcataatgg actggtgatg accctgagcc gcagcgccgg ggcgcatacc
    33121 gacgccgttt tacgtattga gcgcggcggg ttgaaatcac cggatgtgtc ggaaggggaa
    33181 atagcgccac ggctgctgtt agatggtgag ccgttggcgt taagtggtga caagtggcgg
    33241 atttcaccat ggttattagt aaccgatgat acggcaacca ttaccgcgtt tttgcagatg
    33301 attcaggaag ggaaggcaat caccttacgc gatggcgatc agaccatttc tttgaatggc
    33361 ttaaaagcgg cgttgttgtt tattgatgcc cagcaaaagc gtgttggcag tgaaaccgcg
    33421 tggataaaga aaggggacga gccgccgctc agcgtaccgc ccgcgcctgc gctgaaagag
    33481 gtcgtggtgg ttaacccaac gccgacgcca ctctcacgcg aagaacgtaa cgatttgctg
    33541 gattatggta actggcggat gaatggtctg cgctgctcgc ttgatccgtt gcgtcgtgaa
    33601 gtgagcgtta cggcactgac cgatgataaa gcgctgatga tgattagctg tgaagcaggc
    33661 gcgtataaca ccattgacct ggcgtggatt gtgtcgcgta aaaaaccgct ggcctcgcgc
    33721 ccggtacggt tgcgtttgcc gttcaacaac ggtcaggaga ccaatgaact ggaactgatg
    33781 aacgcaacat tcgatgaaaa atcgcgtgaa ctggtgacct tagcaaaagg gcgcggatta
    33841 agcgattgtg gcattcaggc gcgctggcgc tttgacggtc agcgttttcg cctggtgcgt
    33901 tacgccgcag aacccacctg cgataactgg catgggccag acgcctggcc cacgttgtgg
    33961 atcacccgtt aaacaaaatg gcggggacat gtagaccgga taagactcac cagcgtcaca
    34021 tccgacaatc agcatccggc gatcaccatc aggcaccttt cactcccagt atcttatgcg
    34081 cttttgccac aatattctcg gcggtaaagc caaagaacgg gaacagctta tccgccggag
    34141 cagattcccc ataacctgtc atcccgacaa ttgccccttt cagaccaaca tatttgtacc
    34201 agtaatcggc aatacccgct tctactgcca cgcgggcaga aacatttgaa ggcagtaccg
    34261 actcgcgata ttcctcatcc tgggcatcga aaatatcggt cgagggcagg gaaaccacgc
    34321 gaacattgcg accttctccc gctaatttct ctgccgcttg cagggtgatt tccatctctg
    34381 aaccggtggc aatcagaata atatctggct taccgccgct gtctttcagc acataaccgc
    34441 cacgagcaat ctctttaacc tgatccggtg ttcgttctac ctgtgccaga ttctgccttg
    34501 agaggatcag tgctgtcggt ccgttgtggc gctcaaccgc cagcttccag ccaaccgccg
    34561 cttccacctg atcgcacggt cgccaggtgc tgaaatttgg cgttaagcgc agactagcca
    34621 gttgctcaac agcctggtgc gtcggaccat cttcgcccag accaattgag tcgtgggtat
    34681 aaaccataat ctgccgcgct ttcatcagtg ccgccatccg cgcggcgtta cgggcgtatt
    34741 caacaaacat caggaacgtc gcggtatacg gcacaaagcc gccgtggtgc gcgatgccgt
    34801 tggcgatagc ggtcatacca aattcacgca ccccgtagtg aatgtagttg cccgccggat
    34861 cttccttcag cgaaacagaa cctttccaga tggtcaggtt gctgggagcc agatccgccg
    34921 aaccgccgag caactcaggc agcatcggcc cgtaagcgtt aagcgtattt tgcgaagcct
    34981 tacgggtagc gattttcgcc ggattcgcct gcaactcatt gatatatttc tgagtcgttt
    35041 tctcccagtc cttcggcaaa ccgccgctca tccgacgggt aaactcttct gccagttgcg
    35101 gatgagcctt tttataggcg gcaaacttct cattccagcg ctgctgcgct ttttcgcctt
    35161 tttcgcgggc attccaggcg tgataaatct ctttagggat ctcaaatggc ggatggtgcc
    35221 agcccagttt ttgccgtgcc agcgccactt ctttttcccc cagtggtgcg ccgtgcgcct
    35281 cttccttacc tgctttattc ggcgaaccaa agccaatcac cgtacggcag ataatcagcg
    35341 acggcttatc tttcacgctt tgcgcttcaa ggatcgcttc cttcaccgcc tgcggatcgt
    35401 ggccgtcgat ctcgtggacc acatgccagt gataggcttc aaatcgtttt gccgtatcgt
    35461 cggtaaacca gccttcggtt tcaccgtcga tggaaatacc gttgtgatcg taaaaaccaa
    35521 tcagcttgcc cagccccagc gtgcccgcca gcgaacagac ttcgtgagag ataccttcca
    35581 tcaggcagcc gtcgcccata aacacatagg tgaagtgatc gacaatttca tgatccggct
    35641 gattaaactg cgccgccagc gtacgctctg ctatcgccag cccgacggcg ttcgccaaac
    35701 cttgtccaag aggaccggtg gtggtttcaa cacctggcgt atagccgatt tccgggtgac
    35761 caggggtttt cgaatgcaac tgacggaagt tcttcagttc ttccagtggc aggtcgtaac
    35821 cggtcagatg cagcaaactg tagagcagca tcgacgcgtg accgttggaa agaataaagc
    35881 ggtcacggtc ataccaggtt gggtcggtgg ggttatgttt aagaaaatcg ttccacagca
    35941 cttcggcaat atcagccatg cccatcggcg cgccgggatg accagaattg gctttttgta
    36001 ccgcatccat actgagtgcg cgaatcgcat tggcaaggtc ttttcgggac atataacact
    36061 ccgtggctgg tttatagttt ggcggcaaga agatcttcca gtttgcgttg atcaacggcg
    36121 aacagacgaa tgccttcaga cagtttttct accgccatcg catcctgatt gtgctcccaa
    36181 cggaattccg cttcgctcat gggagctggg cgtgggaacg tctgagaagg tgggattaat
    36241 ttacgtacca ctggcgaaac tttttcctgt agctccttca gcaaattcgg tgcgatagtc
    36301 agtcggtcgc agccggttaa ggcgaggatt tgttcggtgc gacggaagct cgcgcccatc
    36361 acaatggttt catagtggtg ttgcttatag tagtcgtaga tattgcgtac tgatttaacg
    36421 cctggatctt cttccaccac atacgggtct atcggcttgc gtgcctgata ccagtcataa
    36481 atacgcccga caaacggcga aatcagaaaa acgcctgctt ctgcacaggc ccgcgcctgt
    36541 gcaaaagaaa acagcagcgt caggttgcag ttaatacctt ctttttccag ctcttctgcc
    36601 gcgcgaattc cttcccaggt cgaagccagc ttgatcagaa tgcgtgattt ctcaacgcct
    36661 tgttgctgat acaagtccac cagatggcgc gccttctcaa tactcttttc tttatcaaaa
    36721 gagaggcgtg catcaacttc tgttgacacg cgaccgggta cgattttgag aatttcagca
    36781 ccgaaattga ccgccagttt gtcacacgct gcgaccacct gttgttcctg ggtcttgcca
    36841 ttttttttac cccaggcgat agcatcgtct attaaatgct catattgtga taatccggca
    36901 gccttgagta acagcgaagg attggtggtg gcatcatggg gatgataatg gcgaatggac
    36961 tcaatatcgc cgctgtctac cacgacagtg gtgaactgtt tgatgccgtc taactcgttc
    37021 ataggaaata ctccttgaaa agtaaagtgt tagatgagtg cgttaattca cacttctgag
    37081 aaatttcgct aaacgcatca aaaaagcata gcagacaggc atggtattgc tggattaagc
    37141 aggtaacatc agtgttatag gattattacc aaaacattat attaatggtg agagtttgga
    37201 cttgctcaaa gtctgtagac tccggcaggg taataatgtg cgccacgttg tggacagggg
    37261 ctgttgccca cacactttat ttgtgaacgt tacgtgaaag gaacaaccaa atggatgacc
    37321 agttaaaaca aagtgcactt gatttccatg aatttccagt tccagggaaa atccaggttt
    37381 ctccaaccaa gcctctggca acgcagcgcg atctggcgct ggcctactca ccaggcgttg
    37441 ccgcaccttg tcttgaaatc gaaaaagacc cgttaaaagc ctacaaatat accgcgcgcg
    37501 gtaacctggt agcggtgatc tctaacggta cggcggtgct ggggttaggc aacattggcg
    37561 cgctggcagg caaaccggtg atggaaggca agggcgttct gtttaagaaa ttcgccggga
    37621 ttgatgtatt tgacattgaa gttgatgaac tcgacccgga caaatttatt gaagtcgtcg
    37681 ctgcgctcga accgaccttc ggcggcatca acctcgaaga cattaaagcg ccagaatgtt
    37741 tctatattga acagaaactg cgcgagcgga tgaatattcc ggtcttccac gacgatcagc
    37801 acggcacggc aattatcagc actgccgcca tcctcaacgg cttgcgcgtg gtggagaaaa
    37861 acatctccga cgtgcggatg gtggtttctg gcgcgggtgc cgcagcaatc gcctgtatga
    37921 acctgctggt agcgctgggt ctgcaaaaac ataacatcgt ggtttgcgat tcaaaaggcg
    37981 ttatctatca gggccgtgag ccaaacatgg cggaaaccaa agccgcatac gcggtggtgg
    38041 atgacggtaa acgtaccctc gatgatgtga ttgaaggcgc ggatattttc ctcggctgtt
    38101 ccggcccgaa agtgctgacc caggaaatgg tgaagaaaat ggcccgtgcg ccaatgatcc
    38161 tggcgctggc gaacccggaa ccggaaattc tgccgccgct ggcgaaagaa gtgcgtccgg
    38221 atgccatcat ctgtaccggc cgttccgact acccgaacca ggtgaacaac gtcctttgct
    38281 tcccgtttat cttccgtggc gcgctggacg ttggcgcaac cgccatcaac gaagagatga
    38341 aactggcggc agtacgtgca attgcagaac tggcccatgc ggaacagagt gaagtggtgg
    38401 cttcagcgta tggcgatcag gatctgagct ttggtccgga atacatcatt ccaaaaccgt
    38461 ttgatccgcg cttgatcgtt aagatcgctc ctgcggtggc gaaagcggcg atggagtcgg
    38521 gcgtggcgac gcgtccgatt gctgatttcg acgtctacat cgacaagctg acggagttcg
    38581 tttacaaaac caacctgttt atgaagccga ttttctccca ggctcgcaaa gcgccgaagc
    38641 gcgtagttct gccggaaggg gaagaggcgc gcgttctgca cgcgactcag gaactggtga
    38701 ctctgggact ggcgaaaccg atccttattg gtcgtccgaa cgtgatcgaa atgcgcattc
    38761 agaaactggg cttgcagatc aaggcgggcg ttgattttga gatcgtcaat aacgaatccg
    38821 atccgcgctt taaagagtac tggaccgaat acttccagat catgaagcgt cgcggtgtca
    38881 ctcaggagca ggcgcagcgt gcgctgatca gtaacccgac agtgatcggc gcgatcatgg
    38941 ttcagcgtgg cgaagccgat gcaatgattt gcggtacggt gggcgattat catgaacact
    39001 ttagcgtggt gaaaaatgtc tttggttatc gcgatggcgt tcacaccgca ggtgcaatga
    39061 acgcgctgct gttgccgagt ggtaacacct ttattgccga tacctacgtc aatgatgagc
    39121 ctgatgcaga agagctggcg gagatcacct tgatggcggc agaaactgtt cgtcgttttg
    39181 gtattgagcc gcgtgtggct ttgttgtcgc actccaactt tggttcttct gactgcccgt
    39241 cgtcgagcaa aatgcgtcag gcgctggaac tggtcaggga acgtgcgcca gaactgatga
    39301 ttgatggtga aatgcacggc gatgcagcgc tggtggaagc gattcgcaac gaccgtatgc
    39361 cggacagccc tttgaaaggt tccgctaata ttctggtgat gccgaacatg gaagctgccc
    39421 gcattagtta caacttactg cgcgtttcca gctcggaagg tgtgactgtc ggtccggtgc
    39481 tgatgggcgt ggcgaaaccg gttcacgtgt taacgccgat tgcatcggta cgtcgtatcg
    39541 tcaacatggt ggcgctggcc gtggttgaag cgcaaaccca accgctgtaa ttctttttta
    39601 actctca
//
LOCUS       DAZFFE010000031        38248 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFFE010000031 DAZFFE010000000
VERSION     DAZFFE010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.031"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(190..573)
                     /locus_tag="ACHJ69_001823"
     CDS             complement(190..573)
                     /locus_tag="ACHJ69_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014821.1"
                     /translation="MNYQTVSELITSSNHNVLIVWDSASEVDGFLNKLNITDYKYYDF
                     SQIYSCSDRTLNDYAVIFIRDALNASEHIIIFNCTGWPDLNNESAVMQFARVARKSGK
                     QLIVAVREQDMKKMEAESGRNIKIH"
     gene            complement(676..1137)
                     /locus_tag="ACHJ69_001824"
     CDS             complement(676..1137)
                     /locus_tag="ACHJ69_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO5014822.1"
                     /translation="MKKYIPVVLFVFSCQVFSADIHGRVVRVLDGDTIDVMLSQHPVR
                     VRLVNIDAPEKKQDYGRWSEKIMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     HFMVRAGAAWVYEQYNTDPVLPVLQGEARQQKRGLWADSAPVPPWVWRSRK"
     gene            complement(1890..2093)
                     /locus_tag="ACHJ69_001825"
     CDS             complement(1890..2093)
                     /locus_tag="ACHJ69_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014823.1"
                     /translation="MAVSSGLSEHSGIYRYQRVGRHAVRQEIRGFFREAAEAWQNAAR
                     LAPCEAWRDFALKRASACRCKCR"
     gene            complement(2245..2802)
                     /locus_tag="ACHJ69_001826"
     CDS             complement(2245..2802)
                     /locus_tag="ACHJ69_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO5014824.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRRRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRE
                     VLLDEVFQRNIPLSHKKLSRALKAITRSESYLCAMKAGACRYDTEGYVTEHITQEEEQ
                     YAQARLEKVRRQNRIKDELRAILAE"
     gene            complement(2857..3603)
                     /gene="traX"
                     /locus_tag="ACHJ69_001827"
     CDS             complement(2857..3603)
                     /gene="traX"
                     /locus_tag="ACHJ69_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFO5014825.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWFQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIVG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            complement(3623..8893)
                     /gene="traI"
                     /locus_tag="ACHJ69_001828"
     CDS             complement(3623..8893)
                     /gene="traI"
                     /locus_tag="ACHJ69_001828"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFO5014826.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGKGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVESLGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGMFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELAMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQEGMAFTLGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGETFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDNVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWVDIPDSSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRSAISEREAALPESVLRESQREQEAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            complement(8893..11091)
                     /gene="traD"
                     /locus_tag="ACHJ69_001829"
     CDS             complement(8893..11091)
                     /gene="traD"
                     /locus_tag="ACHJ69_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO5014827.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLI
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQAEQPQQPQQ
                     PQQPQQPQQPQQPQQPVSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGIS
                     ESGEVVDMAAYEAWQQENHPDIQQQMQRREEMNINVHRERGEDVEPGDDF"
     gene            complement(11142..11879)
                     /locus_tag="ACHJ69_001830"
     CDS             complement(11142..11879)
                     /locus_tag="ACHJ69_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014828.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILEKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVLCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQGENITWSLSSQS
                     PADKYDLINKTSRVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRAAGGYHHRHTGYTWSVSVN"
     gene            complement(12082..12813)
                     /gene="traT"
                     /locus_tag="ACHJ69_001831"
     CDS             complement(12082..12813)
                     /gene="traT"
                     /locus_tag="ACHJ69_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFO5014829.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(12845..13342)
                     /locus_tag="ACHJ69_001832"
     CDS             complement(12845..13342)
                     /locus_tag="ACHJ69_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein"
                     /protein_id="HFO5014830.1"
                     /translation="MIMRCLAHIALVTVIQFIACYLVGWGKAETVFMLFFIVLWQGLF
                     IWLFSQIRKKRNVSDEFKFSKGVWYITMPVSSLLSPLLSLMVFIIGTLYELRRVSGCI
                     SIKKWGQNQLKNQYDGSEKLDFGGIEQPPTTYYNPSTGYPMHGGFDSAGNTFGTRWQD
                     YYDRQ"
     gene            complement(13361..16180)
                     /gene="traG"
                     /locus_tag="ACHJ69_001833"
     CDS             complement(13361..16180)
                     /gene="traG"
                     /locus_tag="ACHJ69_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFO5014831.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVQVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASGDPYTLIFSRPSPLRGVYDSNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASHIMRQNV
                     TMNALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSIGHVLMRNLPMVQTILVG
                     ITIGIFPLLVLAAVFNILTLSVLRGYVFALMWFQTWPLLYAILNSAMTFYAKQNGAPV
                     VLSELSQIQLKYSDLASTAGYISVMIPPLSWMMVKGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNSLSQFGSNRG
                     SSDSVTGGADSTMSSQDSMMASRMRSAVESYAKAHNISNEQATQELASRSTRASAGMY
                     GDAHAEWGVKPKILGVGGGLGVRGGGRAGIDWSDDDAHQASSGSRASNDARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMTWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEEKSV
                     QERMPGADSPQELMKRAKEYQDKYK"
     gene            complement(16177..17550)
                     /gene="traH"
                     /locus_tag="ACHJ69_001834"
     CDS             complement(16177..17550)
                     /gene="traH"
                     /locus_tag="ACHJ69_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFO5014832.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQATVPEIKTAKDFLQKMASDINSMNLSSCQVAQGIVGG
                     LFPQTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNI
                     NIIWDSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKVYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDDREKGF
                     ISSTTVPVFKYLIDPQMLGVSSSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QTVLDSVLENLNQAQQQIAVFQGQVKVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            complement(17537..17929)
                     /gene="trbF"
                     /locus_tag="ACHJ69_001835"
     CDS             complement(17537..17929)
                     /gene="trbF"
                     /locus_tag="ACHJ69_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFO5014833.1"
                     /translation="MTENKKNQEFKIRKIKRNIERSCDNAIKYFELFVVFFVAGLLLW
                     HGMHAFFSAGIDSWKADPELNNLIYMWNILMYAIPYTLYALAAGFLVTSFLSPMYELI
                     FGNIMIFLLKRRMRRENKLREGGNNASH"
     gene            complement(17883..18197)
                     /gene="trbJ"
                     /locus_tag="ACHJ69_001836"
     CDS             complement(17883..18197)
                     /gene="trbJ"
                     /locus_tag="ACHJ69_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFO5014834.1"
                     /translation="MRNKQVVLLIAGISGIATGIIVSLNIPFIRQGLFYPASPVEIVV
                     SLCLTFSVSVVFFVGAIVGWISVSEIYYSCMACLNESSEISEETYDDRKQKESGIQNQ
                     KN"
     gene            complement(18187..18732)
                     /gene="trbB"
                     /locus_tag="ACHJ69_001837"
     CDS             complement(18187..18732)
                     /gene="trbB"
                     /locus_tag="ACHJ69_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFO5014835.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQMYGEEKGAK"
     gene            complement(18719..19003)
                     /gene="traQ"
                     /locus_tag="ACHJ69_001838"
     CDS             complement(18719..19003)
                     /gene="traQ"
                     /locus_tag="ACHJ69_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFO5014836.1"
                     /translation="MISKYRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            19084..19344
                     /locus_tag="ACHJ69_001839"
     CDS             19084..19344
                     /locus_tag="ACHJ69_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFO5014837.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSLPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYVYFCELFRSHWIAVWFIL"
     gene            complement(19400..19741)
                     /gene="trbA"
                     /locus_tag="ACHJ69_001840"
     CDS             complement(19400..19741)
                     /gene="trbA"
                     /locus_tag="ACHJ69_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFO5014838.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSAIWSG
                     VMWFFIISLVK"
     gene            complement(19755..20498)
                     /gene="traF"
                     /locus_tag="ACHJ69_001841"
     CDS             complement(19755..20498)
                     /gene="traF"
                     /locus_tag="ACHJ69_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFO5014839.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(20491..20748)
                     /locus_tag="ACHJ69_001842"
     CDS             complement(20491..20748)
                     /locus_tag="ACHJ69_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFO5014840.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAFVISPVIIWSWDTVKETTAGEML
                     AACFVIFYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(20775..22625)
                     /gene="traN"
                     /locus_tag="ACHJ69_001843"
     CDS             complement(20775..22625)
                     /gene="traN"
                     /locus_tag="ACHJ69_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFO5014841.1"
                     /translation="MKRLLPLLLGLAVSVAQADSNSDYRAGSDFARQIQGQGTGSIQG
                     FKPQESIPSYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEINRSEFTNYTCERDTMVEEYCTRT
                     ASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFL
                     NSRYTFMNTTFNVGLANGQSDTYPLSGATGLQVTQGQVLTGSGCTANGNCLPHGNGDR
                     KVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLTGTECSEPGGTKT
                     GVMEGKTWNITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLH
                     EYATYSCESKTSGKVMVCGGDVFCLDGECDKAQSGQSNDFAEAVSQLAALAAAGKDVA
                     ALNGVDVRAFTGQAKFCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLT
                     VSVGEFCSKKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHPDCRGIT
                     VDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(22622..23044)
                     /locus_tag="ACHJ69_001844"
     CDS             complement(22622..23044)
                     /locus_tag="ACHJ69_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO5014842.1"
                     /translation="MALTKKQREKLRMKFGGRCAYCGCELPEKGWHADHVQAVLRKSE
                     QCMKAAEKRIFRLKSTGDVFRPEADCPENLVPACAPCNLLKTTYSLEMFRTQVSLQVE
                     RGRRSSVNFRTAERFGLIQVVNKPVVFWFEQYQRESEV"
     gene            complement(23069..23440)
                     /locus_tag="ACHJ69_001845"
     CDS             complement(23069..23440)
                     /locus_tag="ACHJ69_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014843.1"
                     /translation="MTLPAGYYRIDPDIRALVAAMNVHGFRTYASCQGHGFPVTKLPP
                     YIAFVCPVKKAALLEQRLRQDAESMMPRLLWGWSVGASFNSDLQLCFRLQPEGPHHWY
                     HRYCRRSLRADFRTLVRLLNP"
     gene            complement(23437..24075)
                     /gene="trbC"
                     /locus_tag="ACHJ69_001846"
     CDS             complement(23437..24075)
                     /gene="trbC"
                     /locus_tag="ACHJ69_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFO5014844.1"
                     /translation="MSRKIFVLFALTTGLFISAHASENVNTPENRQFLKQQETLSQQL
                     REKPDTQLQAWAEQQVRANPLQHSDNQFLDELVRKQQAAQKDKPQQGAVYFVSFSIPE
                     EGLKRMLSETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGHDIIRGNLRVRQALEKVATAGDCRQVARALLNNPGDKQK"
     gene            complement(24102..24620)
                     /locus_tag="ACHJ69_001847"
     CDS             complement(24102..24620)
                     /locus_tag="ACHJ69_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014845.1"
                     /translation="MSFLSFLRKISNKEKEIFISEERFFSLLERVSAFDVVREWAENG
                     GAYYPLLECRRLTDIEDVRKSEDILNRLEFIDRRFREFISIMSEYNRNDKSKNRWERE
                     LSEYPPEKIYQVRREGNHLSLKNYGDDITLNRDTAVATVQITVSRKCITQEEMDELYA
                     LSERLKEKCAGN"
     gene            complement(24683..24991)
                     /locus_tag="ACHJ69_001848"
     CDS             complement(24683..24991)
                     /locus_tag="ACHJ69_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014846.1"
                     /translation="MEKKLSSKYIHMYMSISRVFIRLTGNVLKLFPFAMVICPVVWFY
                     LCPEHIISDIVNDWQDADTLEKILLVKLYLKTVLILTVVSVVVLSLSSDKFMSVKRRK
                     "
     gene            complement(25018..26010)
                     /gene="traU"
                     /locus_tag="ACHJ69_001849"
     CDS             complement(25018..26010)
                     /gene="traU"
                     /locus_tag="ACHJ69_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFO5014847.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(26007..26639)
                     /gene="traW"
                     /locus_tag="ACHJ69_001850"
     CDS             complement(26007..26639)
                     /gene="traW"
                     /locus_tag="ACHJ69_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFO5014848.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(26636..27022)
                     /gene="trbI"
                     /locus_tag="ACHJ69_001851"
     CDS             complement(26636..27022)
                     /gene="trbI"
                     /locus_tag="ACHJ69_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFO5014849.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(27019..29649)
                     /gene="traC"
                     /locus_tag="ACHJ69_001852"
     CDS             complement(27019..29649)
                     /gene="traC"
                     /locus_tag="ACHJ69_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFO5014850.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(29775..29988)
                     /locus_tag="ACHJ69_001853"
                     /pseudo
     CDS             complement(29775..29988)
                     /locus_tag="ACHJ69_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(30016..30234)
                     /locus_tag="ACHJ69_001854"
     CDS             complement(30016..30234)
                     /locus_tag="ACHJ69_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014851.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIESHGGRG
                     FGNTGECIEVKTNKPLNNVMDTLNGGGQ"
     gene            complement(30314..30787)
                     /locus_tag="ACHJ69_001855"
     CDS             complement(30314..30787)
                     /locus_tag="ACHJ69_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014852.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNQINNILAYCACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEQDIRRLWQEKVDAMQESATRQREQDTIRKFTEGNKSE"
     gene            complement(30780..31193)
                     /locus_tag="ACHJ69_001856"
     CDS             complement(30780..31193)
                     /locus_tag="ACHJ69_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014853.1"
                     /translation="MHKSENEKIDFILDEHEQIFRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQETQRKHYA"
     gene            complement(31186..31407)
                     /gene="traR"
                     /locus_tag="ACHJ69_001857"
     CDS             complement(31186..31407)
                     /gene="traR"
                     /locus_tag="ACHJ69_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFO5014854.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(31542..32057)
                     /gene="traV"
                     /locus_tag="ACHJ69_001858"
     CDS             complement(31542..32057)
                     /gene="traV"
                     /locus_tag="ACHJ69_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFO5014855.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNSDFECDATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTTSVQAVAATTPSRSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVAPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(32057..32374)
                     /locus_tag="ACHJ69_001859"
     CDS             complement(32057..32374)
                     /locus_tag="ACHJ69_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2689 domain-containing protein"
                     /protein_id="HFO5014856.1"
                     /translation="MNMRNINVITARAVPDKNVSDDFMHAVLSNCTTRIALPAPKKFS
                     SESLPHNFNMAAVGVMKKGMSMSLFSSPGNTVVSGQPCAGKTNLTVELISAVVESNKK
                     GEG"
     gene            complement(32361..32927)
                     /gene="traP"
                     /locus_tag="ACHJ69_001860"
     CDS             complement(32361..32927)
                     /gene="traP"
                     /locus_tag="ACHJ69_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFO5014857.1"
                     /translation="MAGDALTFRVAFVVRWLLWVVRFVVIWPLATMALMALFVLWKDN
                     TTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAADAADYVRE
                     TDRSLMQLVTAFWATLALMYMSFAAGTGKYPMWPGKMKCVRVGTADEHLKEVYTEDAS
                     LPEKIRKCPVYFHDDRTNRSNGDKNEHA"
     gene            complement(32917..34368)
                     /gene="traB"
                     /locus_tag="ACHJ69_001861"
     CDS             complement(32917..34368)
                     /gene="traB"
                     /locus_tag="ACHJ69_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFO5014858.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPAPPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALEQSQSTAEEGNPESPVPNPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGDKNPLGGDAGKEGGNGG"
     gene            complement(34368..35096)
                     /gene="traK"
                     /locus_tag="ACHJ69_001862"
     CDS             complement(34368..35096)
                     /gene="traK"
                     /locus_tag="ACHJ69_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFO5014859.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPAVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADSVWTGNHLKMVRFVVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(35083..35649)
                     /gene="traE"
                     /locus_tag="ACHJ69_001863"
     CDS             complement(35083..35649)
                     /gene="traE"
                     /locus_tag="ACHJ69_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFO5014860.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNQMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(35671..35982)
                     /gene="traL"
                     /locus_tag="ACHJ69_001864"
     CDS             complement(35671..35982)
                     /gene="traL"
                     /locus_tag="ACHJ69_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFO5014861.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGIIT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(35997..36356)
                     /gene="traA"
                     /locus_tag="ACHJ69_001865"
     CDS             complement(35997..36356)
                     /gene="traA"
                     /locus_tag="ACHJ69_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFO5014862.1"
                     /translation="MNTVLSVQGASAPVKKKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGDLMAKGQETVKATFGKNSSIVKWIVLAEVLVGAVMYMMTKNIKFLVG
                     FAILSVFIAVGMSVAGF"
     gene            complement(36389..36784)
                     /gene="traY"
                     /locus_tag="ACHJ69_001866"
     CDS             complement(36389..36784)
                     /gene="traY"
                     /locus_tag="ACHJ69_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-bindin protein
                     TraY"
                     /protein_id="HFO5014863.1"
                     /translation="MKRFGTRSATGKMVKLKLPVDVESLLIEASNRSGRSRSFEAVIR
                     LKDHLHRYPKFNRAGNIYGKSLVKYLTMRLDDETNQLLIAAKNRSGWCKTDEAADRVI
                     DHLIKFPDFYNSEIFREADKEEDITFNTL"
     gene            complement(36883..37572)
                     /gene="traJ"
                     /locus_tag="ACHJ69_001867"
     CDS             complement(36883..37572)
                     /gene="traJ"
                     /locus_tag="ACHJ69_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003979562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer transcriptional regulator
                     TraJ"
                     /protein_id="HFO5014864.1"
                     /translation="MYPMDRIQQKHARQIDLLENLTAVIQDYPNPACIRDETGKFIFC
                     NTLFHESFLTQDQSAEKWLLSQRDFYELISVTEMEAYRNEHTHLNLVEDIFIQNRFWT
                     ISVQSFLNGHRNIILWQFYDAAHVRHKDSYNQKTIVSDDIRNIIRRMSDDSSVSSYVN
                     DVFYLYSTGISHNAIARILNISISTSKKHASLICDYFSVSNKDELIILLYNKKFIHYL
                     YEKAMCIINMR"
     gene            complement(37759..38142)
                     /gene="traM"
                     /locus_tag="ACHJ69_001868"
     CDS             complement(37759..38142)
                     /gene="traM"
                     /locus_tag="ACHJ69_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFO5014865.1"
                     /translation="MAKVNLYISNDAYEKINSIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDDE"
BASE COUNT         9051 a        10730 c         8770 g         9697 t
ORIGIN      
        1 atttaatcat caattctgac acatcatcat atttcatata ttcaccatta tgagataaaa
       61 tctggataca ggtggttccg gctttccccc tgtgccacca tttcccggtt tttattcaga
      121 aacgccgggg acggggaaaa cttgcttttt cccgtccccg gggttggaca actgagcaac
      181 gcgaaggcgt cagtggattt ttatgttcct tccagactct gcttccattt ttttcatatc
      241 ctgttctctt actgcgacta tcagttgttt tcctgatttt cgtgccactc tggcaaactg
      301 catgacggca gattcattat tcaaatcagg ccatcctgta caattaaaaa taataatatg
      361 ctcactggcg ttcagtgcat ccctgataaa aataaccgca taatcattta atgttctgtc
      421 agaacaactg taaatctggc tgaaatcata atacttataa tcagtgatat tcagtttatt
      481 taaaaaacca tcaacttcag aagctgagtc ccacactata agaacattat gattagacga
      541 tgtgattaat tctgatactg tctgatagtt catttgttca cctttcatta acagagaaat
      601 aagttaataa tgtaatatgc ttcatgcttt taaatatcgc atttcaatga gaactcaata
      661 gccacagaaa tatcattatt tccgtgagcg ccatacccag ggcggaacag gagcgctgtc
      721 tgcccacaga ccacgtttct gctgtcgtgc ttctccctgc aggacgggaa gaacaggatc
      781 ggtgttgtac tgctcataaa cccatgcagc gcctgcacgg accataaaat gattaacatt
      841 cattccgtct ggtgcataaa cctgcccgag aatacgacca taacgatctc gctgaaaata
      901 tgtgacagta accgtttttc ccgcaaccag agatttcatt attttttcag accaccggcc
      961 atagtcctgt ttcttttcag gtgcatcaat attaacaaga cggacacgca ccggatgctg
     1021 agataacatg acgtcaatgg tatcaccatc cagaacacgc acaacccgtc catgaatatc
     1081 agcagaaaat acctgacatg aaaaaacaaa taataccact ggtatatatt tcttcatttt
     1141 actctccgtc actgattact tttcgtgatt gcatgaattc catgccggga tgaataacgg
     1201 ctcacgtatc acgccaaaaa taaaccgttg aattgtgatc aattcgacaa aataagatga
     1261 ggtggcttaa tacacatatg gctatgtgtt acttatgtgt tactaaaaac gctggtcagc
     1321 cagcctgacc agacacataa gaaacaaagt ctcatgcatt attcagcctt ctgatacctg
     1381 gagtaacgga ggctgctttt tctgtcacgt atttcccgtg acggcgttac cggtttccgg
     1441 gggtaacatg tgacggagat tatattttta atctccgtgt attcatcata cctgctctta
     1501 ctgataggat aactgtgtta tatcgcaggc taccggatgt ggcgctgaat ataacggtca
     1561 tagataagtc atacatgccc tgctcagggg attagcgata agacggaccg ggagagtcat
     1621 ctcccggttt ttctttatcc tgccgcgaat acagcccggc tgtctgtcag attcaccgga
     1681 cataaccgta ctgttatgca tgctactgtc accttctgct tcttgaaatt catcacattt
     1741 tttatccctg ctggcttacc cgggatgtga aatatgttaa ctctgtgatg tccgtttgaa
     1801 agacagggca actccttctt acaccactat aataataacg atctgccata taagcctgat
     1861 cccccggagc tccgcgacgg gggttatttt tatcggcact tacaccggca tgcagatgcc
     1921 ctcttcaggg caaaatcccg ccatgcttca cagggtgcca gccgggccgc attctgccag
     1981 gcctcagcgg cttccctgaa aaaaccacga atctcctgac ggacggcatg acgccccacg
     2041 cgctgatagc gataaatacc gctgtgctca gataatccac tggatacagc cattttttat
     2101 cccccctctt gacccggctc ccggattatg tcagaaacat tctgaaacat ccctgaacgg
     2161 ttgggtgttt tctccatcaa aactctggat ttctgttttc ccgatttttt ctcgctgttg
     2221 ccctctgtca tacagagggc tcttttattc cgccaggatc gcccgcagct catccttgat
     2281 ccggttctgt ctgcgcacct tttccaggcg cgcctgcgca tactgttctt cttcctgtgt
     2341 gatatgttct gtcacatagc cttccgtatc atagcggcag gcgccggctt tcattgcaca
     2401 cagataactt tccgaacggg tgatagcttt cagcgcccgg ctgagttttt tatgagagag
     2461 cgggatattc cgctgaaaca cctcatccag taaaacctcc cggataccac aggcaagaag
     2521 cctgggcgtg ttaccgtcaa acaatcccgg ccaccagggt ttcagagtgt tcaccgcctc
     2581 atccagcgag ggcagcgtca gataaatgga cagcgcctgt ctggcctgcg ctttttttgc
     2641 cgccagctcc gcttcccggg ctgctttctc ggccagcttc tgctttttca ccttccactt
     2701 tggtggcgtg gtgacattga tgatggtttt ccgcctgcgg acaggcgtct ccccttccgt
     2761 tttccgcttc agtgtcagta ccggtcgctt ctgctctgtc atgctgtgtt ctgcctctga
     2821 cgaatcatcc tgttacggtc aaagatacca cacccgtcac agcgccagaa cgcccagcac
     2881 agccagatga caggcgtaga ataccgggaa aaaatccccg ggccagaaac ggggtaatga
     2941 ttttcctgca caggacacca gaccaacggt cagcaccgtc atcacaagac cagccaccgc
     3001 cgctgcatca ctggtggcaa ggttaagcgc cggaataaca gccagcaggc aggcgaccag
     3061 cgccagacgt tccgctctgt cttccgcccg gtacagccgg tggctgactg ccagcatcag
     3121 cagcccgaca atgccgtaac tggtgccgga caaaggcccc cacagggcca tcagaagaat
     3181 ggccgctgct gtacgccacc cactgcgcgt ttcacaccac gtcagcacct gtgccgccac
     3241 tgcaaaggca aacaggatat tcccctcata ccagggaaag ccggccagat aatatgcgaa
     3301 ctgggcaata attccccatc cccacagccg gttaatggcc ggttgccgga tatgcgcatg
     3361 acgggacaga ttcagccccc acaccagggc aaacagcgga aaggctcctc gcccggccag
     3421 aaacatccat tcctgcttca gctggaatat cagattgatg tggtccagta ccatcagcac
     3481 cagtgccacc gttttgatga tatcccgctg tccgggtgac cagaccagaa atgactgaaa
     3541 ccaggtatcg gtccgtgcag ccagtgaatc gttacgtgtc gtgttcgtgt tatccgttgt
     3601 catcagcgtt tatcttcctg tatcagtctc cgcccagggt tttctctttc tgcaggtcac
     3661 gaaccatatc ccgctccatc tgctgcagtc gctcctgcag cagattttcc cgggcaacct
     3721 cccggaccgc ttcctgctcc cgttgtgatt cacgcagcac actctccggc agcgcggctt
     3781 cccgttcaga tatggcagac cggtcacgct cctgtccggc aatctccctg acagcctgct
     3841 ctgttttgcc gtccggcagg tcaggtttgt tctctgccat tttacggaca atttcatccg
     3901 cacggcgttc cgtttcacgg cggatggcct cttcagcctg ccgctgtgcc agtacctctg
     3961 ccgtgaccgg ttcgccattt ccggcacccg gctggacgct gctgtccggg atatccaccc
     4021 acacgcgacc accggtgatg gcaccgggat tccacggacg accttcaccg gcaattctta
     4081 ccaccacacc gctgtcagga ttatcacggg caatccggac accatcctgc atattatcag
     4141 ccagcaggct ctctccgtta cggctaccct gcagggccac gaactgcgca tccccgctgc
     4201 ctttcacccg tccttcaccg ctgaatcccc gcagcccgtt tccgtcatcc gtggtcagtg
     4261 ggttcagcca gataccggcg gacttgccgt tacggtcaaa cgccggcagt gccacatacg
     4321 gctgcggata tttacgtccc ggtgcaataa accgtgcagg gctgtctccc ccggccagcc
     4381 ctgcctgacg gagaacggca cggcctgccg ccacgtcccg cagttcccgt gccgtactga
     4441 acagccgctc tgcattcatg acctcccggt ccggtttcgg ttcaaacaca tcgtgggccg
     4501 ttcctttctg tacggcattg ttgatggcat ccgtccagcc ctgacggtta tcggtgtaca
     4561 cctgcacatg ctgcttcata cgcgacaggg ccacgtaggc tgactcaaag ccggccatca
     4621 gtttccggtt accttccgtg ccttcaagcg cgatggcaaa ggtttcactc gccccctgcg
     4681 caccgtgggc ggtgatggca taggccaggt caatatgttg ctctgcccgc tcctgaccgg
     4741 ggcgaatcac ccgggtctgc tggccgtccg acagcgtgac actgtcacca gaaaccgctg
     4801 tcaccgtcca gacgctgttg gccacataac cgcgctcccg gtcactcttc gtgaagcgca
     4861 tccggtcgcc ggtccccacc cggattttgt ccggggtgta cagtgtgaca ccttcagcca
     4921 ccgcctcccg gggcgaaatc agccgcgtgt taccttccgc atcctccagg gttatcagcc
     4981 cgtcatcctt gctgataccg gcaatccggt gatacacatt atccaccagg gcaagggcat
     5041 ccgggttatt ctcccaggtg gagagacgac gcagctcccc gtcacgtata ttcgctgtgt
     5101 tcaggacagg caccatgacc tgctctttcc ccagctcacc ggccttttcc cgtgcatcat
     5161 gaatcatgct gttcagtacg cgccggtcct cattcaggtg cgtgacaatc agcgtctgct
     5221 cccgtgcttc cggtgtcctg ccggtatagt cgcggacaat ggcttcatac agtgtcatgg
     5281 ggacatccgg aaaagtctca cctttcagca tcgccttctg ctgcgcttct gccagtttcg
     5341 cttcctggct gtgactgaac tccgtcacgg agtgctccgg tgcccatgcg ccctcctgac
     5401 ggggaacctg agacggtttc acactctcaa gcccggacag tgccctttcc acatcccggt
     5461 taatcaggct gtataccgcc tcccgcagtt ccggcgtctg acgcacaatc tccttcatga
     5521 tgaccacatc ggcagcactg cgcgtctgct ggagacggaa aggctgaccg ggcgcgatgg
     5581 cctgcagctg gtccgtgtca ccgctggcca cagcacgacc accaccggcc gcaatcaggg
     5641 cgtatgcccg tgccatgtcg gtattgccca ccatagagct ctcatcgagc agaaacagcg
     5701 tgttgctgaa atccggcgtt tctccgctgc gctgctgcag ctgcgtgtca tgcagaaagg
     5761 acgccagtgt ctgtgcatcc acgccggcgc tgcgcatctc accgaccgca cggtgcgtgg
     5821 gccccagccc cacaacccgg ggacgctcac tctccggcag catgttcacg gctgacatca
     5881 ccgcccggaa ctgagtggtc ttacccacac cggcataccc ctgtaccacc gtgaaacggt
     5941 cggacgtttc cagtatcata cgggtggcgg cacgctgtcc cgacgttaac gtctccatga
     6001 gttcgccggg tactctctcc atcagcggcg tgaccgcctc cttgccttcg agaatatggc
     6061 gaagaatgct cttttctgcc tcatacgacg cacgggaaac cagcaggcct gtgccatagc
     6121 cttttgccac atccacatac agtaagtcac cgcgttttat ctgcgcattg atttcccctc
     6181 ccagttcagt aaaaccggtt ccttctgcag caaacgactt cgcctctgtc agcaggtcca
     6241 ccatgctgaa ggccaggttt ttactttcca gcacaggaag ggccagatga atggcctgct
     6301 gtgccggcgt gtgaagcccg gctttctgca gactgatagc ggtttccagc aatgtctcac
     6361 cggcacgcgc ctttatctgc tcagaaacca ccgtaaagga cggatggcgg gcgagttttt
     6421 ccgcagtacg ggtttcatcc agtgaggaat acagccggac atcacgaccg ctgcgggcca
     6481 gaccgttcag ggtggcgttg tccattgcca tctgtgtgac ggaggcaaac accgtcgcac
     6541 tgtcactgac ggaatgcccg ggcgtttcca cccagccgtt ctccagcttc agtgccgtga
     6601 acggtgaatc agccacaggc agggtggccg gttcagcccg ccccggcaca acaaccgtca
     6661 tcgcatcttc actgacggat gccacctgca ggcggtcacc gccggagacg cggagtccgg
     6721 gaattttccc tgtcaccctc agtcgctcgc cgtctgccac cggcattttt tccggccgga
     6781 acagcgacca gctgctgtcc agggaggaaa tacgcaccac ctgcgtttcg ccctgcgcat
     6841 cccgcagggt caggctgtga ctctgcgccg tcacccggtc gataacatag cggtcatgac
     6901 tgcgtgtctc cgggttccac tgctccatca ccatccccgg tcggtacata tcccgcagat
     6961 aacggctccg gctgtccagc cagaccggtg acagggcggt catggtcacc tccgggtgtc
     7021 cgagcacgcc ctgtgttttc agctcactgc gaatggcctg tgtcagtatg gcctgttccc
     7081 gtaccccgct gacctgtgcc acgctctctt ctccggcttt cacgctggcc gcaaaatctc
     7141 cggccagccg gtcatagcgg acattccggt ccggttcact gatgatggtt gccggtcgct
     7201 gttctccgcc ctgccagcga tacgtgttca ccccggcatc cttcatggcc atcagcgcac
     7261 tgcctgtacc ggttcgctgc ccgctgtcgg ttatcaggac ctgtacgtta tgacgtgcgg
     7321 caccgtccag cagggttaac gtctctttca gggagagttt ttcgccctgg tcgacgataa
     7381 cggtactgcc cagcgtgaag gccatgccct cctgcagctg acgacgtccg gttatcagct
     7441 caccggacag ccgttcatcc tgcttcaggt tcatctgcga gcgacggtca gcggcgataa
     7501 tctgcacctc ccgcccctgc tcccgggcca tcattgccag ttcagccacc cgctcacgct
     7561 gcccggctgc accgccctgc ccggacacaa tggccagcga cgggcgatcc tgtgccagaa
     7621 cactgacggc atcgctgtaa ccggccgtcc gggggacact tttctccgga tgtacggtca
     7681 cccggttctg tttcatgatg tcacgactga gtgcccggac tgacagctca tcgagcacat
     7741 gaatcccgga cgtgaacatc cccttctcac gatcgagggg gataagctgc tcacggctga
     7801 tggcctcatc gataccggcg cgtgcccgtt caatcacacc attttccggc ggcagtatgc
     7861 cgaccgtcct ggccagcacg tccgtgtacg tgaactgcac ttttcgttca cttaatccgg
     7921 caatcgcctg tgtcaccgcc tgctgcacat ccggcccgtc ctgtgaagct ggcccgggcg
     7981 cctgcgtgcg ggtctccgca cgctgatccg ccgcgtcacg atatgcccgg atgtcgaacc
     8041 cggtttcctt cagcgtctgc atccattcag ccattctgac ttcaggatcg acgtgctgtt
     8101 tggattttcg cgtatccagt gccgccacat cccgggattt cagcgaggca tcttccccga
     8161 cggcctcccg gatagcctgt gagcgtccgg aaaaggcctc caccggtacg cccggcatct
     8221 cccacatacc gtgcttaccc accacttcgg tttcatagcc cagtgactca acctgctctt
     8281 tcagtttttc ccggtagagc ctgccaaagg caatctgatt agcgtacaca ttctcaatga
     8341 accccgtttt ccccactttg tcactgctca gtgtcttcca ctcaccgtta tgctgcgtga
     8401 cattagccac caccgcatgc gtgtgtaact gtggttcctg atcgcgactg gtgtcgtggt
     8461 taaacagtgc catcaccaga ttaccggtca gtaccgtttc tgactgtccg tccgtcatca
     8521 cccgtgtgga ggccagcgcc tccacctgac gaacagcaaa gtccacggcc tggttatgtg
     8581 catcaatcag gcgcttatcg ccacctaaca tggccatcat ggagacactt ttgggggcgg
     8641 agaaggtcag gtcgtagccg ggacgatgct tattactgcc atcctgcatg cggcttagat
     8701 ccgctccgtc cggcagcctt ccctccagaa gacgggtaaa aacatcctta tcgacactgc
     8761 cctgcagtcc cagctgttca gcccccttgc cggcccagcg ttctcccatg ctgcccagta
     8821 catagtaatt atccttgtcg gtataatagt taccggcact tccggccgat ctgacctgcg
     8881 cgatactcat catcagaaat catctcccgg ctcaacatcc tccccacgct cccggtgcac
     8941 attaatgttc atctcttcac gacgctgcat ctgctgctgg atgtccggat gattttcctg
     9001 ttgccatgcc tcataagcgg ccatatccac cacttcaccg gattcactga tcccgggtgg
     9061 cagttgctgt tccatctctt cttccggttt cattttcagc tcctgctcga tcccccctgc
     9121 cggaacattc acacctgaat ctgacttctt atcgttgatg gcaggagaca ccggctgttg
     9181 cggctgttgt ggctgttgtg gctgttgtgg ctgttgtggc tgttgtggct gttgtggctg
     9241 ttcagcctga gtcacatctt ctccggaggc aacctccggt tcaaagaggc tggccatctg
     9301 acgaccttct gcttcccttg cggcaagtac ggcactcaga cggttctcca tttccgggtt
     9361 gatgtcacgc ggaataaact ccggagcgac cttcggtcgt gcctgatatt tcagagagag
     9421 tttgactgcc ggatacggtc cgggcagagt gacatagcag gtcagatccg gcagagactg
     9481 aatgtcggaa taactgacca gcgtctgacg ctccatatcc ttaccggtcg ataccccgtc
     9541 acgtaccggg tcagcaccgt aggaatactg ctcgctggct ttcaggtgct ctttctcacc
     9601 aatttcaccg gcagcgaact ctgcaatctt atggctggga gaacggaaaa aggcacgggt
     9661 gttcatgacg tcaaacagcg tggccgccgc tttctcaccg tagatatctt ccagctgggc
     9721 ataggactgg ataccaaaca cataacagcc accgaactta cgggcttccg gcaggatctc
     9781 caccaggtcc ggcagtttgt gtaacgtggg taactcgtca cagaaaaacc acacacgacg
     9841 gttacggttt tctcccattg ccagcagacc acgaatggca atggacagcc acatggagat
     9901 caccggcttc agggaggcat gggtgtcggc gttcgacgaa ataaacagcc agccgttttt
     9961 ctgatcttcc cggacaccac gcatccagtc acggatggta aaagactcac cgttatgctc
    10021 aatcccctgc aggtaacgga tggctttcac gtagttggtc agcacagcac ggatggaaat
    10081 cgccgttttc tcaattttct cttccaccag gttggccgcc ggtgaattac gcaggaaggt
    10141 acgcagtttt tcgattttga tggaaagcag tgtgtccacc agtttgctgt agctgcggtt
    10201 ggggtcattg cgcatcaggt acgccgcttc cgcaaaaatg gttcgtcctg aaccctgcca
    10261 gaacgggtcc tcttttgtgc ccatcgggat cagcgtgttc gcggtattat caaaatccgg
    10321 ctgtgtcagg cactccttcc acaaatccca ggcagcacag cgtgcatcca gcggattcag
    10381 gattttatcg atggagggat catagtaact tttaacaaat tcccctgaac ggtcataaat
    10441 caccaccata tcaccgcgct gacgggcgta gttggccaga cgacggataa cctccgactt
    10501 accggccccg accgtgccgt gcagacagaa gttctggatt tcggaatccc ggataatcgg
    10561 caggtcgcca atccggatat cggagtcctt gccgtctttt ttcagcatcc gggcaacatc
    10621 tttcggattg tctgtcagct gacgaccacc tgtgacttca ttttcgctct gttgtttacc
    10681 ctgacgcccc agaatccagg aaacaacaaa gaaggtaatc aggcaaataa ccagtgccac
    10741 aactgtggcc agaacgaatg cggaccatag ctgctctccg caccagatca tatatttatc
    10801 atgcagtacc tgggcagcgt tcatccggaa ggttttgccg taatactgga tctcatatac
    10861 tggctgggac ttgattaaat cccgcatgcc ttccagcgtg gtacaccacc agtaaataca
    10921 gccgttcaca aacgtctgcc agcttatttt tacccataaa atcagaccaa cgagtatcca
    10981 gaaaaaaata aacaggcagt aaagcatgat attggcgatc tggctgaaca tgcggatacg
    11041 catggacgca atctgaccgc cctgggtcat atcctttgcg ttaaaactca tgatgaaact
    11101 ccgggaaaat ctggaacagg gcagaaaagg atgatgtggg gttaattcac agaaacagac
    11161 caggtgtacc ctgtatgccg gtggtgatat ccgcctgcag ccctgccgga agagaccttc
    11221 tctgtaacaa tgtcagtcag tcgcaatact tcagaccagt cactcccgtg cggaaactgg
    11281 atctgaataa aaaggcgtac ccgttcacgg tgatccagaa ctgtgtccgg ttcataaaaa
    11341 cggccactgt caggagaaac acgggaggtt ttattgatga gatcatactt atcggcagga
    11401 gactgcgatg acagagacca ggtaatattt tcgccctgat gaccatcaat acgactgctc
    11461 cccggcgtcc atgcaatttc ctgcgcactc gccacgatga tctccctgga gacttctgcc
    11521 agctttctgg gtgtacagag taccggtgcc agcacacaca gggacaacaa attacagctg
    11581 accgcctccg gtttaagcaa attgctcccg gatttttcag gaaaagatat tttttccagt
    11641 atttcagtta attccttatg cctgagagaa ttaataaagt acatgataag catcatgaga
    11701 aacaatataa caataagccc tccgataacc actaaaaggt cagtatcaat attattgctg
    11761 tacagatagt ctttagtggt cactgtatta tcctgtatca ccggggggta ttcagttaca
    11821 ggcttctcag taaattgatt attctcacca acatattcag cgatattgtc tgatgtcata
    11881 atgactttct ccggaagatc ccggcttcgc cggcaacact tcacggtcgc acaaagaatt
    11941 tgcacaaccg ctcagtgttt tcatatttat ttcggaaacg ccgggaggtg acaaaattta
    12001 ttttgttaca tcccggggtt ggacaacgag caacgcgagg cgtcagcaag gaggccggtc
    12061 agatcagcct cctgacgagg ttcagagaat atttgcgatt gatttggcca gctggtcttc
    12121 gagaacaggc ttcgcctctt caaatttcag gttaaccttg ttcgcatttg agaccacacg
    12181 ggtctggtat ttatgctggt tacctgtttc agtactggtc tgaattttcg caccggatgt
    12241 gccctgacgc agggcggcaa cattatccgt tgtcaccgtt gccttagtac gctctgcaat
    12301 ctgcacatcc gtgatcatgg tatagttcac atcttccacc atcgcatctg cagccatacc
    12361 caccagaccg gcagcaaggc ccacaccgag tgtggcaccg gcagaatttg agttatatcc
    12421 ggtaataccg gcacctaacg ctgcaccaac agctgcgcct tcataaccac ggttcagcca
    12481 tccctgagac tcccgcagat ccatcttatc ggccttcagc acattcgcct gaatccagta
    12541 gtaggcttta tccggagaag tcaccacctg atatcctttt gctttcacag catcagcaat
    12601 tttgccctgc agcccactca tgtctttatc agacgtgttt ttgatctgca gaaataccgt
    12661 gcgttcgctg gcgggttcaa gccagatggt ctcactcatc tgagtcttca cctcaaggtt
    12721 acgcttcttg attgctgtgc tcatcgcacc acaccctgaa agggccagag tggaactgac
    12781 cagtgcaacc atcatcaatt tttttgtttt catcaaatta ccttttttac aaactacagt
    12841 tttcttattg cctgtcataa taatcctgcc aacgagtccc aaaagtattt cccgctgagt
    12901 caaagcctcc atgcataggg tatccggttg aagggttata gtaggtcgtt gggggctgtt
    12961 caatgccccc gaagtctaac ttttcactcc catcatattg gtttttgagt tgattttgcc
    13021 cccatttttt tatgcttatg caaccactaa ctcgccttaa ttcgtatagt gtaccaatga
    13081 taaaaaccat caggcttaat aacggagaga gtaaggaaga tactggcatc gttatatacc
    13141 aaacaccttt actaaactta aactcatccg acacattccg tttttttcgt atttgagaga
    13201 acaaccatat aaacagtccc tgccatagta ctatgaagaa cagcataaaa actgtttcgg
    13261 ccttccccca accaaccaga tagcaggcga taaactgtat caccgttacc agagcaatat
    13321 gagctaaaca tctcattatc atctcccaaa cacagcatta ttacttatac ttatcctgat
    13381 actcctttgc ccttttcatc agttcctgtg gactgtcagc cccgggcatt ctttcctgaa
    13441 ccgacttttc ttcattatat ttattattct gagataatgc ctctgtttta tggtgattct
    13501 gtagttcaga atactgtcct cttacaatat tttcctcgcc acgtatgctg ttctgagtat
    13561 cacctatatt ccctttatat tctgtgacca tattatcaac ctgatgtttt acatcattac
    13621 ggatattact gtcctgcgtt ctttgctcaa taatggcctg atgtccctga tggtcagcaa
    13681 taatatcctg actgccacca cccgaaggta cgctctccat tcctttacca atatcgccac
    13741 gggattcccg ccaggcatta tcaacaccag gctgtacctg ttcctgcaca aaagaccagg
    13801 tcatcgcccg acgacgttct gcaatctccg gtgaactggt atttgtcaga atagcttcgg
    13861 catcctgtgg cgcgtgtttc attacatact gtgcaaactg ttgcgacaag tcttcactca
    13921 tctgcccact catgctttca gtacgggatg ccatttcagc atattcatgg ctgcgggtca
    13981 tattcgtcgt gtactggtcg taactctgct tcgctgaatt cagggctgca gacaactgat
    14041 ccacacggga atcagcatta ttgtcagtat ggctgccgga ctcactgacc ttgcgactgg
    14101 taaagtaatc gctggcctct ttaaagtctt gtgtagctct ggcatcgata tcatgacgag
    14161 catcattgct ggcccgtgaa ccactgctgg cctgatgcgc atcatcatca ctccagtcaa
    14221 tgccagcccg accaccgcct ctaacgccta atcctccacc aacaccaaga attttaggtt
    14281 taacccccca ttcagcatga gcatctccat acatcccggc agaagctctc gtacttctgg
    14341 atgccagttc ctgagtcgcc tgctcattac tgatattatg cgccttcgca tagctttcca
    14401 ctgcactgcg catacggctg gccatcatgg agtcctgtga gctcatcgta ctgtcagcac
    14461 cacctgtgac agaatcactg ctcccccggt tagaaccaaa ctgactgagc gagttccagg
    14521 cactggcaat actgctgctg aaaccatgca gggcactttc tgctctgttc gacgcctccc
    14581 gggccatttc ctgttgtgcc gccgcaatct gacgcgttgc attgatgcca accggtaacc
    14641 gggacatcgc cccgctcgca tccatcacca tattaccgtc acgggtctgt gtggcagttg
    14701 cgccactgcc ggtctggtac atcatctgac caaacgacgt ggtgctgttg gtgctccagc
    14761 tgaagccgtt cacgttttcc gtctgcatgt tgccgtagga gtaattaccg tcaaccacac
    14821 tacctgccgc actggcagtc gggctgatag cagaggaagc gaaatggctg tacacgctgg
    14881 aaaaacctgc gccaaggcct tttaccatca tccatgacaa cgggggtatc atgacagata
    14941 tatatccggc agtggacgcc aggtcggagt atttcagctg tatctgagag agttcagaga
    15001 gcacgaccgg tgcaccattc tgctttgcat agaatgtcat ggcactgttc agaatggcat
    15061 acagcaacgg ccaggtctgg aaccacatca gagcaaacac atagcctctc agaacagaca
    15121 gcgtcagtat gttgaagaca gccgccagta ccaggagcgg gaagatacca atggtaatgc
    15181 ccaccagaat ggtctgcacc atcggcaggt ttcgcatcag cacatgacca atggagacat
    15241 gtgccagacg ctgtttctcc atcgacgaag tcgtggccag actgaccagg ctggcggtat
    15301 caccgttacg ggctgcatta ctggtgatac cctctttcag ggcattcatg gtgacgttct
    15361 ggcgcataat atgactggca gactggctgg agccgtagaa ataactgtaa ctgtcactca
    15421 ccagttgcct gaataacagg tccgggtccg gtctgccgcc aaatatctgc tgcacataat
    15481 aatgccaggt cttgcctccc gtctttgtat cgagattcag cctgtctttc agcgtgaccg
    15541 acgcatcttt acaggttatg aaattattat tgctgtcata aacgccccgt agcgggctgg
    15601 gccgggaaaa aatcagcgtg taggggtcac cggaggccat caggtcttcc agcgtgtatt
    15661 tgtggttcag gtaaatatcc cccagcacgc agttctggac gtagtcctga aacagattga
    15721 tgatttccgg attccgggac agaaaatcgg tgcttttcac aatcaggttc gccccgaaca
    15781 gcatgcctgt cttgctgtag gtggcgctgt caggctgggt gaatatcatc tcgtaactgg
    15841 ccaccattgc atgcccgata cgggtcgtca gtgaaagtgg catcgccagt ccgaccggca
    15901 cattatccac ccggtgaacc tggaccaggt cactgttatc aataatctgc accgatgtac
    15961 ggacattaac cagcaggctg ataagcacaa acacggccac ccagcccagc aaatccatca
    16021 cgttgtgccg ctgtacccac atcacggcca ccgcgagaac agacaatgtg agcgcaattt
    16081 tttcgatgga atcccacgtc cgggtcccca taaaggcggc aatggcgttc aggttattgc
    16141 gtaaccactc accaccggca atcacataga cttcattcac agcgtgctcc ctccgaagtg
    16201 atagttgttc tggtaacggc tgagcatgcg ggcggacagc tgctggcgca tgtagctcat
    16261 ctgacggtca acaaccagca atgcatcctg ctggaccttt acctgcccct ggaaaaccgc
    16321 aatctgttgc tgtgcctgat tcagattctc cagcacactg tccagcacgg tctgtggata
    16381 gttcccggtg gcaatcatgg cccgggcctg ctgaagcagt tcctgaatgt actggagcat
    16441 gatgtcgtag ccgatatagt ccgtcagctg gtaaaccacg ctgctggaca caccgagcat
    16501 ctgcgggtca ataagatatt taaagacggg gacggtggtg ctggaaatga agcctttttc
    16561 cctgtcatcc agtggagtat cactcaccgc tttattctga atactggtga gcagttttgt
    16621 aatctgtgat ttcagggctt tatcccgggc gatggtcaca tttgcatccg ccaccacctt
    16681 cagacacttg tccgaatcgt tgcagtgata aacttttgcc gttcccccct ccataatcgc
    16741 ccggaggata tcgcggtcag tggtacgcgc cggcaggggg gtaatctcgc tgtcttcccc
    16801 aaaaatcagc gtaccggtga gcgtcataat aaattctttc agttctttgt taccatcaag
    16861 catcctgttt ttggacagcg agtcccagat gatattgatg ttcttcagca cccgttcctt
    16921 atctttgtca ctggccttgt cacgaacaga cgatgactgt cctccgaccg tgcagccctg
    16981 acgggacgcg gcccagtcag caaaaatatt gctctcaccg gcaatgtcct gacagacctt
    17041 ctgctgtgac acctgagtct gaggaaataa tcctccgaca atcccctgcg ccacctgaca
    17101 ggagctgagg ttcatactgt taatgtcgct tgccattttc tgcaggaagt cttttgcggt
    17161 tttgatttcc ggcaccgttg cctgcagggc aaggtcaaaa aagtaaccgg cagcgttgct
    17221 cataatctgc ttaacaaaac gctgcaactg atcgccatta ataaaactga acgagccgag
    17281 ataggcgtcg atgccgccac atccggcatt gatatccggc agcgtcatgg aaatcagctg
    17341 gatatttttg acctgggtgc gggcatacag ggagccacca taggcatacc cggcggcctg
    17401 cccctgccag acgcccggtt gtgtggtatt ggaagcaaat cccagcttat taaagaactg
    17461 attcatgtcg ctgttcacat ccgctgacgc tgcgggtgtg accgtcagta aagcggcaca
    17521 aagaaccaga agaggtttaa tgcgaagcat tattgccacc ctcccttaat ttgttctccc
    17581 ggcgcatacg gcgtttcagc aggaatatca tgatatttcc gaatattaat tcgtacattg
    17641 gggacagaaa agatgtaaca agaaacccgg ctgcaagcgc atataacgta tacggaatgg
    17701 catacatcag gatgttccac atataaatca aattatttaa ttccgggtca gctttccacg
    17761 aatcaatacc ggcgctgaaa aaagcatgca taccgtgcca taaaagcaat ccggcaacaa
    17821 agaaaaccac aaaaagctcg aagtatttta tggcgttatc acaactgcgc tcaatattcc
    17881 gtttaatttt tctgattttg aattcctgat tctttttgtt ttctgtcatc ataagtttcc
    17941 tctgatattt ctgatgattc attcagacag gccatgcagc tgtaatatat ttcagatact
    18001 gaaatccatc ccacaattgc ccccacaaaa aacacaacag aaacagaaaa ggtaagacac
    18061 agcgaaacga caatttccac agggctggcg ggataaaaaa gcccctgacg gataaagggg
    18121 atattcaggc tgacgattat tcccgtcgcg atgccggata ttccggcaat gagtaacacc
    18181 acctgcttat ttcgcacctt tttcctctcc gtacatctgc agaacggtat ccatccgcgc
    18241 cataaaactg gcggcatccg ttgccccctg caaaagcggt aatgcctcaa gtgtgttgac
    18301 gttgaccagg aaggtggtcg gtgtggccac cgggatattc gggaagaagg tctgcatcac
    18361 gtccggtggc accggtaatg cttccggaaa ggcagtatcc ccctgaccat ccagggtgta
    18421 gggaaaaacg gaaaagccgt actgctgtgc cagctgcttc agtaccgggt caaactggtg
    18481 acagtaaggg caatgcccct gcataaacag caccaccttc cagtcggcca ggttaacctg
    18541 cctgccgttg ctgaggcgga accagcgggg agccggcttt tctgttctgg ctgatgtgtc
    18601 tgctgccggt tgtgcaggct gcacggccat accctgcgga ttccagagtc gttcaatttc
    18661 atcccgggtg gaggcctgca cacctgtggc catcatcaac aaaatgaccg gcagtaattt
    18721 agtgagagac atgtccgccc tcctgctgcc cttccatcat ggcctgttgc ctggcttcca
    18781 gtgcctcacg ctcttccagg ctgacacgtg caatccatgc atccagcacc tggtatgcgc
    18841 cgaacagcac aaggatggcc gcaatcaact ccgccagaaa aaaagccatc cagggtttgc
    18901 tgtaaacaag acgggcgacg atatgaaacc agacacccag ggaaaatacc cacattcccg
    18961 taatatcaag ccggggtaaa gagaatctgt atttacttat catgttaaac tccggttatt
    19021 atttacatga gagtattcat gtctgtaata ttcgcgtttg tgtgatatta atcagaggta
    19081 caaatgaaaa agttatcttt taaagaaaaa ctggaaatag tgagaaacat cataagagag
    19141 tcattatcag gtaatgcggc ttttgcttta ctggtgtatt cattattgca ctccctcccc
    19201 attaaaacct ttgctgattt atttgtgacg atactgttat ttatagcgat tatcgctgtt
    19261 gtgatatggc tatttctaat catctatgtt tatttttgtg agttattcag aagtcactgg
    19321 atagcagtct ggtttatcct ctgatctgtg gtaatcaacc tcactattgg atttggcatt
    19381 tatgacaaat tagcaccagt catttcacta atgaaataat aaaaaaccac atcactccag
    19441 accatatggc tgacaacata atacaaacaa acatccttct gaaactgtca aacttcgtga
    19501 taaccggtat ggtactgact gccattgaaa caaccggtac ggcacactga ttcataaccg
    19561 caccacaaag aaacaagaaa ttatctggca gcatacatac acttatgaca tttactacaa
    19621 agaagacaaa cgagctcagc agataaactc ctttattatt tggtcctgta aagaaataac
    19681 caacaacccc cacgataaac aggctggcac ctgtaaaaaa acacaaatcc cctgtatgca
    19741 tatataacct ttggttaaaa attcggttta aaatcttcag aaacgttcag gaactgtttt
    19801 gccaggtcgt cctgtgaaat aaagccgtat gataacgggc gaacactgcc ctgtttcggg
    19861 tcaaccagca tcatggccgg gaaatatttc acaccgaggc gctgcgcctg cccctggtca
    19921 gtccgggaat ccggcaacag cggattaatc acgccatcca cggaaacggg aataacactc
    19981 agaccatacg tatcccggaa gccattaatg acctgcgcca gttgcccgtc gatggggtcc
    20041 tgcccccggt aaaaaaacat gatgccgtag tgttcagcca gtttcgcaat ggcctgtcgc
    20101 tgctgcgcct ggtctgccgc cagctggttc cgtaccgtgc cgttgtaatg gctgtactgc
    20161 aggttatagt ccagttcggg atgtgccagc atggcctttt tagcactcat ggtgaaaagc
    20221 ccggcctgct gagtccagta attctgcagc cggaaatatt taacaaaatt atccacgccg
    20281 ggatacagaa tggcttcgta cagcgcccgc tttgttgccg tctgcagcgc ggccagtttc
    20341 tgcataatat ccggctcctg acggggggcg gcaggtacag gtttattttc tttttccttc
    20401 ggatttattt tctcgttata ccactgccag cctgcatctt tccctgacgc cggaaaaata
    20461 aagcagcaga gtaacagtgg cagtaatgct ttattcatct tcattccttt catcattttt
    20521 tctttcatca tctttctgca ggccagtcag ggcagaaaaa caaaaataca gaataaacag
    20581 gagcacaccg gagtaaaaaa taacaaaaca agcagcaagc atctcacctg cagtggtttc
    20641 ttttaccgta tcccacgacc agataattac cggggaaatg acaaacgctg taatcaaaag
    20701 ccggataaga aaatcaataa aacggttcga cgtgaatatg actttcatca tttcccccgg
    20761 ttattttcca taccttactg tcctgcctgt ttcagctggt cagcaatctg ctctttcact
    20821 ttctgcgtca gaacgccact gtccgggatt ttctggttat tcatcaggtc ttcgtagaag
    20881 ttagtgaaat ccagtctgtc gaactgaatt ttctgcagct catcaaccgt aatcccccga
    20941 cagtcaggat gcttcgcact gccaaacccg atacgcaact gcccgttacg cccctgctgc
    21001 tggactatct gcgccagctt cgaatcaaac tggcagtagc tgcgcttttt ctgcagacag
    21061 acacccagca ctttcttcga acagaactct ccgacactga cggtcagttt gtttgatttt
    21121 gctttagcca gggccttttc atcactgctg catttggcca gcccgatatc ctgtccccag
    21181 ccgctgtcct tacagcagtt gctgtatccg gctgcagcct tcttacaaaa tttcgcctga
    21241 ccggtgaaag cacggacatc caccccgttc agcgccgcaa catccttacc tgctgcggcc
    21301 agtgccgcca gctgagaaac ggcttcggca aaatcattac tctgcccgct ctgagcctta
    21361 tcacattcgc catcgagaca gaagacgtct cccccacaga ccatcacttt cccggatgtt
    21421 tttgactcgc aggagtacgt ggcgtactca tgcagacagg ttccctcatc ggaataaaac
    21481 gcgcactgac gtgaggccag cgtacaggcc ggattatcga catatttctg acaggtgccg
    21541 ttatctgcgg actgagtgac atatttatcc cggtatgccc agcatgcctg tgtgatgttc
    21601 caggttttac cttccatcac tccggttttc gtaccacccg gctctgaaca ctcagtgcct
    21661 gtcagcacgc catccgcttt attaaacgga caactctcca cccactccac ccggggaacc
    21721 cattctttat cacggacctt catcctgagt ttcagcgtaa aggtggaagc cccactgaca
    21781 agcgattcat agactttcct gtcaccattc ccgtgcggga ggcaattacc gttagcagta
    21841 cagccactac cggtcagaac ctgcccctgt gtcacctgca aacctgtcgc cccggacagg
    21901 gggtaagtat ccgactgccc gtttgccagg ccgacattaa aggttgtatt catgaaggta
    21961 taacgggagt tcaggaagaa aaaagcggcg tatacagaca atgatgcact ttccactgta
    22021 ccggttaccg gagccgtgac actgaacacc agtttcagcc cgttcatact gaaccggaac
    22081 tgtgagtgcg gtatcgtcac ttcacgatat tcatccgtgt atttccagtc cccggtgata
    22141 ctggcagtcc gtgtgcagta ctcctccacc atcgtgtccc gctcacaggt atagtttgtg
    22201 aattcactgc ggtttatctc ctgcgcactg cactgctgtc cggtatttcc cacaatgctg
    22261 tcagcccggt tcaccacatc cctgccggtc tgaatgaacg gtgcatccgg tgacagaatg
    22321 tctttcggct tgttcatgaa ggactctgtg atggtttttc cggtttcacc ggtcgcccat
    22381 tccgtggtgc cgtcattttt caggccgcca tccccgccgg cagtcacgcc accgtaatat
    22441 tttgtctcgt ccggattcgc gttatagctg gggatactct cctgtggctt aaagccctga
    22501 atactgcctg taccctgccc ctgaatctga cgggcaaaat cagaacccgc ccggtaatca
    22561 ctgttgctgt cagcctgtgc cacactgact gccagcccca gcagcaatgg cagaagacgt
    22621 ttcatacctc actctccctc tgatattgct caaaccagaa cacgactggt ttgttcacta
    22681 cctgaatcag cccgaaccgt tctgcggtcc tgaaattcac gctgcttctg cgcccccgtt
    22741 caacctgcag ggacacctgc gttctgaaca tctccagcga atatgttgtt ttcagcaggt
    22801 tgcacggcgc acaggccggg acgaggtttt ccggacagtc ggcctccggt ctgaaaacat
    22861 caccggtgga tttcagcctg aaaatgcgct tttctgcagc cttcatgcac tgctctgact
    22921 tgcggagcac cgcctgaaca tggtcggcat gccagccttt ttccggcagt tcacagccgc
    22981 agtacgcaca acgaccgccg aatttcatac gcagtttttc ccgctgcttc tttgtcagcg
    23041 ccatcaatgc ctccttacgg gaacagattt acggattcag taagcggacc agcgtcctga
    23101 agtctgcacg cagtgagcgg cgacagtacc ggtgatacca gtgatgaggg ccttcaggct
    23161 gaagccggaa acagagctga aggtcactgt taaaagaagc cccgacggac cagccccaca
    23221 gcaaccgggg catcatggac tccgcatcct gccgcagtcg ctgctcaaga agagccgcct
    23281 tttttaccgg acagacaaag gcaatataag gcggcagttt cgtaacaggg aaaccatgcc
    23341 cctgacagga cgcatatgta cgaaatccgt gaacgttcat cgcggccacc agtgcacgga
    23401 tgtccgggtc aatccggtaa tacccggcag gtaaagtcat ttttgtttat ctccggggtt
    23461 attcagcaac gcccgggcca cctgccggca gtctcctgcg gtggccactt tctccagcgc
    23521 ctgcctgacg cgcagatttc cccggatgat gtcgtgcccc tggctgcaaa acaccaccag
    23581 ggccggtacg ctgcgaatgc cgtactgtga aaacagcgtc gggtcgatct gaacaccatc
    23641 agttgcgccg tctttcacca gggacaacac ggcttcagcg gtggtcttca ggtcattgtt
    23701 caccatgccc cgcagtgtgg ccggaatacc gtagtgccgg gtttcgctca gcatccgttt
    23761 cagcccctct tcgggaatgg agaaggacac aaagtacacg gcgccctgct gtggtttatc
    23821 cttctgagca gcctgctgtt tacggaccag ttcatcgaga aactggttat cggaatgctg
    23881 aaggggattc gccctgacct gctgctctgc ccatgcctgc aactgtgtgt ccggcttttc
    23941 acgtaactgc tgactcagtg tttcctgctg cttcaggaac tggcggtttt cgggcgtatt
    24001 cacgttttcc gaagcatggg cagaaataaa cagtcctgtg gtcagggcaa aaagtacaaa
    24061 tattttccgg ctcatatcat ctcccggcat agcgatataa tttaattacc ggcacacttt
    24121 tctttcagtc tctcagacaa tgcatataat tcatccattt cttcctgagt aatgcatttt
    24181 ctgctgacag taatctgcac cgtagccacg gcggtatctc tgttcagagt aatatcatca
    24241 ccgtaatttt ttaatgacag atggtttccc tctctccgga cctggtatat tttttccggt
    24301 ggatattcag acaattccct ctcccacctg tttttagatt tatcattacg attatattca
    24361 gacataatgc tgataaattc ccgaaatctt ctgtcaataa actccagtcg gttcagtata
    24421 tcctctgatt tcctgacatc ttcaatatct gtaagccgac ggcactccag cagaggataa
    24481 tatgctccac cattttcagc ccattctctg accacatcaa aagcactcac cctttccagc
    24541 agggagaaaa aacgctcctc actgataaaa atttcttttt ctttattact tatctttcgt
    24601 aaaaatgata aaaaactcat aacgcacctt tatttatatt ttacatatgc acacacggtt
    24661 atatttatga gaaccgacag actcatttcc tgcgcttaac agacataaac ttatctgacg
    24721 aaagagacaa gacaacaacg cttaccactg tcagaataag aaccgtcttc agatacagtt
    24781 tgacaagcag tattttttcc agtgtatctg catcctgcca gtcgttaaca atgtctgaga
    24841 taatatgttc agggcaaaga taaaaccaca ccacagggca tatcaccata gcaaaaggaa
    24901 acagttttaa tacattgcct gtcaaacgaa tgaacactcg ggatatactc atatacatat
    24961 gaatgtattt actgcttaat ttcttttcca tttaaaatat tcctcttttt gtttaagtta
    25021 caggaataca cagttacgtt tacgccacat caggtagccg aagtttttct ttgtgttggg
    25081 cggatttttt cccgtctccc agcgcatcac actgcgcccg aacggatggc actgtccgct
    25141 gtccggatac atattcacca tctgataacg ccagcgttct ttgggcagga ttggagacgg
    25201 atattcatta cagacggcgt tatttttccc tatggtttcc ataatcatgc cctgacggtg
    25261 tagtttgaac gccatgcgtt cactgaccag cagggaggac tgcaacggac tggactcatt
    25321 actcacccag ccattgaacg ggtacatact gccctgcgaa ccggcacacc agaacagaac
    25381 atcgagaggc atattaaagg cactggcaat ggcatctgct gcgcaggccc cctgtgctat
    25441 cggattggca aagatgacag cttccggatt gagaatggtg gtcaggctgc tgtccgtcca
    25501 ggtggggtca atttcagaaa gataagcgat atccaggtca ccaccttcca gacagcccag
    25561 cgatgtgatg atgttaagcc agtacgtcag cgggtatttg taccagtgaa cgtgatagaa
    25621 cgccccgttc acctgcttct catccttttt cgccgttccc tgtgccgttt taccaaaagc
    25681 cggcaggctg aagcccaggt tcaccatgca tcccggtgac cgggtgacgt ccgtcagcgc
    25741 catcggctcc cagtaaccaa tggccagccc gatacgcctg aacagcggcg gcggtgccgg
    25801 acaaatctga atgggcatcg acgggttcgc cgtgtcgggg accttaccct gactgacttt
    25861 gatactgccc agcgagagcg ggaaaataca gctccagcag atatccgtga tcgggtttac
    25921 aaaacgcccc tcacaggcag aatccgcaga tgccgcaggg acatgaccgg cgagaaggaa
    25981 taacatcagc agccacaggc ttcgcttcat tttctgccct cctctgccgg aataaattcc
    26041 accttcagga agcgatcgcc gggaacggcg ctcacccgtg ccgggacctg atcaatgccg
    26101 aggcgctggc agagcacacc gttctggtca aagtaaacac ggctgtccag agacttctgc
    26161 atctccggga tactgccctg cacgaggata attttgctct ccagtgtggg cggcgtctgg
    26221 cgtttcatcc aggccacctg cgccggatca tcgccattga tgaaatacag cgtctggtta
    26281 aaaggcacat actgcagcgg attcatcact tcaccctgac gggcaaacac ccgcccttcg
    26341 ttatcccgga tatccgcagc cagtctgacg gacgggtcaa acaaccggct gccgtatttc
    26401 tccgtgcgtc cgataccggg cacggcaggg ggccgcagac tgttgcggat cacccgctcc
    26461 ttaaaggcat ccattttccg gcccatctca ccggactgct ccagtgccgt caggcgctgc
    26521 atgatcaccg tcagcatgtc cggctccttt accggccaca gatcgcccca ggtgccaaga
    26581 tcggcggcgg ccacactctg cccccatatc agcagggcaa tcagcccccg gcatctcatg
    26641 gttccgctct cattcgccgg gcaatatcct gctggatttc ccgggtgata tccggtgccc
    26701 cctgtaccac ggcaggcgac accagaatga ccgcgtggtg tttctgctgc cacgcctgca
    26761 ggctggcttc cagtgccgtg ttaaaacgcg ctgaaagggc ttttgactgt gcttctgaca
    26821 gttgtttctg actggcgctg tcaaaaaatg catccacagt ctgtttcatg ttgaaggcgg
    26881 cagtcaccgg tgcgttcagt ctgacaatgc cgtaactgac ggctgcgttc agtaacacca
    26941 ttgccaggca tcccggcacc gtccaccacc agtggctgcg tcggggggct gtaacatcag
    27001 tcgttttttg cgttgtggtc atgcaacact cctgtatttc tcatgttctt ccagccaggc
    27061 ttccagcgaa gccatctccg gccctgactt cttccacgcc agctgccaca ccgcctcatg
    27121 aatactcagc ccctctttac gtttctgctg cacaaactca aaatccgggc cgtcagagct
    27181 gtacatggcg cggcttaacg ggtccacaaa caggcggtgc caggaggaat ggttttccac
    27241 ctgcagcagg aaggaactga accactggtc tttggccgca ccaaacttgc caatcatgtc
    27301 gcgctgcagt ggctggaact gatccgggaa taactggtta tatttcgcga actccttcgc
    27361 actctgtttg aggataattt tgtaggagga gttaccccat gccgcacggg ccgcactgga
    27421 ggccttgtca gagtcaaagt cgacgatgtt ctgtgtgatg gtgatatagg caccggtatg
    27481 gcggcgacag gtgcggtaac ctttttgaat aaactcaccg actttacgat ttttgaagtc
    27541 cagcagacgc cagccttcat caatgacgtt cagtttcttg agagtacgcg gcgtgcggta
    27601 catcctgttc tcgatgtaga taatcaggga gaacatcacc gcaaccagca gtgacggacg
    27661 gtcttccagt ccgcccagct ccagcaccac catttttgcg tcatcccgca gggacggctc
    27721 atcagagtta aaatactggc cgtaagtgcc gttggcagtg tactggtcaa gcagcacaat
    27781 catttcgtcc agacggctgc ggatggtcgg tgacccggca tactgctcgc tgtcactggc
    27841 gtttttcagg aaatccacca cgtcatcaat acgtgccctg ttctctttgg ccagccagga
    27901 ggccctgacc gcctgcagca gcagaccttc atgcacttca tccaggttac cgttggggct
    27961 ggccatcact gacaactggt cacggacacg ctccgctgac tggtcgatat cggtgatgtt
    28021 cgcaaacggg ttaaaacgca gggtttcacc gtccagatat acgcccccca tgttctcaca
    28081 cagggacttg tagccatccc ccatgtcgaa caccacggca aagcccccgg agtccagcac
    28141 gctgcggata agtggctgta tcagtccggt tttaccggcc ccggaggtgc cacagaccgc
    28201 catgttgtag ttggtgttat tcatcccctt aaagaagata tcgataaacg ccagctggtt
    28261 acggtaggtg ggcgccagca gacctgccgg tgtcaggggg ttatccgcca ctaacggcat
    28321 caggttggcc acattaaagc tctctgcgcg ctgaaccaca ccggcctctt tcagctgttt
    28381 aaacagccct ttcccggcca taaagggcag gcaggtcagg aaattgcgca tgtggttaaa
    28441 gcgcggtgaa atcagctcaa aaccgttttt acggaagctg ttcaggatgt cctgctccac
    28501 ttccagtgcc gtttcattat tgtccttgca gaaggctgtg atgttgagga agtaggacac
    28561 gacagaagac tgtccggagc ccagccgctg gcgcagttcc ccccactcct tcgcctcttt
    28621 ctccacggac ggaaaccatt tggcatagga ggtcttcgac ttcttctcca ggtccatgta
    28681 cttcaggttg gcttcgctgt gggttttcac ctggtcttcc accaccagag tcagcgtcag
    28741 gatgaacggg caggaaatgg acatttccgg gttcagcaga ttgctgtagt tgtcggccat
    28801 gttccacagg aaggcgattt ccgggttacg ggccagatgg aaattcagga tgcgggccgt
    28861 gctgttcctg ccgttctcac gcaggcccag cgtcaggtaa tcagcccgga cattcaggtc
    28921 aaaactgtcc tccacacact gataattcag atcagaatac gggtccagct gacgtctttt
    28981 cgggtacagg gagtccggat tatggttaat catctccccg acaatctcga tgaaagcctg
    29041 tgcatccact gtctgtgtgg tgatttttgc gccgtgaaaa gaggcacgga tgattttcac
    29101 cagattttcc atttccagaa tgtcagcccg gctttttttc ttcgagggag aacagtacga
    29161 gataaacacc cggtaatggc gcagggtcag gggcagattc atcccctccg gcagcgggaa
    29221 ctgtgtcgct gccgctttca tataataggc acgggtaatg gcgttaaacc gttcagcctg
    29281 ttcacctgac caggagaact cacgcagccc gtattcaatc ctgtcaccaa ccagctgact
    29341 ggacatcaga tggatacaca acggaatacc gcgcggcagt ttggtgcgca gcatgtgatc
    29401 gagtgcctcc acaatggact tattcgcccc attgatggga atggcttcca gcataaagcc
    29461 catcgtggtg tcattcatga acagaccgga ttcctggttg taatcacggt acggcagcag
    29521 acggctgaac tgcgggaagc tcatttcgcc cagaacttca ttggcctttg cggattcgtc
    29581 aggcagtttc agtgctgtga cgagggagtt aaccgcctga gtgacggcct caagtgggtt
    29641 attactcata tgagtattcc ttagtgcgtt taaaaaagga aaatatcagt ctgtgaccgt
    29701 ggatatatca ggcagtgcca ttcctgaaac cacagcacaa ataagagatg gaggctttta
    29761 acaggaaaca aggattaatg ttcctttgct gaatgagcag catttaatgc ctgaagttgt
    29821 ttttcacgta tcttatttcg ctcattttct cttacccgtt tcacctctgc catttcgttt
    29881 ctttctctac acgctgatgc aagctctttt gccttaccaa gacttaaaca tagccataac
    29941 gccaacaaat ataaaagcca gaacagattc taaatatatc gacttcattt cattttcctt
    30001 tgaagcagta agcactcatt gcccaccacc attcagtgta tccatcacgt tgttcaaagg
    30061 tttatttgtt ttaacttcaa tacattcccc tgtatttcca aatcccctgc ctccgtgtga
    30121 ctcgattgtc cgaagaacct gcacgcatac gtccgtattt ggcatgggtg taatattcca
    30181 gcttccgcca ccgttaatat tttttatcag tacaagaaaa ataaaggtat gcattcttta
    30241 ttatctccgc ttgtatatta atcactgaat atcccttcag ttaacttatg aggtcataaa
    30301 acactgcaga ttctcattcg gatttattcc cttcagtaaa cttccgaata gtatcctgtt
    30361 cacgctgacg ggtggcagac tcctgcattg catccacttt ctcctgccag agacgccgga
    30421 tatcctgttc atcccgacca gtcagttttt taccggacaa caaacggtta agcagttcct
    30481 gtctcgcatc cggtacgtct tcagtcagac gcataagctg aagcaggctc tgacggctga
    30541 ggaccatgag tgattccggc aggagagtga agaagaaaat aaaaaagcat accgcaccac
    30601 agcaggcaag gaccgcaaac aggattttca gatatatacc atcaacgccg aacgcagcaa
    30661 taatacaggg aatccatgca atacacgcac aatacgccag gatgttattt atttggttat
    30721 gtctgcgata tatctgttga tgttcatcca gaatagaatc tattttttca gcttctgatt
    30781 tatgcatagt gtttcctctg cgtttcctgc cagagacgcc ggatatcctg ttcatcccga
    30841 ccagtcagtt ttttaccgga caacaaacgg ttaagcagtt cctgtctcac atctggtacg
    30901 tcttcagtca gacgcataag ccggagtaat tcctgacgac tgagtaccat gcgtgattcc
    30961 tccggtacgc tgtaataatt aaaaatacga ctgaataccc cacaacagca ggcaacggac
    31021 aggcacaggg atttcagata caggccagaa acacagaatg tggcaacacc acaaagaata
    31081 gctataacta aaaagccctt acccagtgcc atacacaaag aaccctgttt tctgaatatc
    31141 tgctcatgct catccagaat aaaatctatt ttttcatttt ctgatttatg catagtgttt
    31201 cctctgcctt tcctgatacg cctgacattc aacgcacaac gtcacaccgg gaaatatttt
    31261 cctccgggct tccggaatag gatttccgca tgcttcacag agataaacag gaataccatg
    31321 aacatttatt ttctggcgta tccggtttat tcctgtcatg gtcagttgtt ctgtcactga
    31381 atatgcttca tcggcttcat cactcacgga atattcctcc cgacatgttc tgacacgcta
    31441 ccgcccatcc cttttcacgc acagggcact cgttttacag gcagattttg aatacagaaa
    31501 gcccgccggc attgccggct gatatacaac ttcagggcca gttaattaat acgtggtttt
    31561 ccccacgcag acggtttaat aacaaaaaac acactggatg gctgatgata aacatcctga
    31621 ttatcaatat aaggcgctat ccataatgca gccgtctgct cacccgttct tagcggacgg
    31681 ggaggtgtta ccggcgcaac tgaactgacc ggaacaaccg ttttcacttc ccgggcagcg
    31741 gtaaacagcg ggcgtggtgc cagcagtttc tgctccggat gtgctgtcac cgcaggcctg
    31801 ctgcgggacg gcgttgtcgc cgcaacggcc tgcaccgacg tggtccggaa gttcccttca
    31861 gccaggcgcg gcagtgatgc cgcaaccggc tttgcttctg aggactgctc cagttttttg
    31921 gccttctcat tagcctgctc catcgtcata caggtatctg acgtggtggc atcacactca
    31981 aaatcagagt tcgttcctgc acagccggaa agtaataagg ttcccagcag aggaataaat
    32041 aaagaaatct gtttcattac ccttctcctt ttttattact ctccacaaca gcagaaatta
    32101 actctactgt cagatttgtt tttcctgcac agggttgtcc ggagactact gtatttcccg
    32161 gagaagaaaa taaactcatc gacataccct tcttcatcac tccgacagca gccatattaa
    32221 aattgtgcgg caatgattca gaactgaatt tcttcggcgc aggaagtgcg attcttgtgg
    32281 tgcagttact gagaacagca tgcataaaat catctgatac gtttttatcc ggcacagcgc
    32341 gggcagtaat aacattaata ttacgcatgt tcatttttat ctccgttgct tctgtttgtt
    32401 ctgtcatcat ggaaataaac agggcatttc ctgatttttt ccggcaggga agcgtcttcc
    32461 gtataaactt ccttcagatg ttcatctgcg gttcccaccc ggacacattt catttttccg
    32521 ggccacatcg gatatttccc ggtgcctgcc gcaaacgaca tatacatcag tgccagcgtt
    32581 gcccagaatg cggtgaccag ttgcatcagt gaccggtcag tctccctgac ataatctgca
    32641 gcatcagccg cgcggtaatg gcagatatcc tgtatttctg atctgcctga agaatcagag
    32701 gaagaaaacc agcattcact gaccgggaac tgtccggcag gtgctgtttg tcttacgaag
    32761 ttaatctcct taaccagtaa tttccccggc gtggtgttgt ccttccataa aacaaacaga
    32821 gccatcaggg ccattgtggc cagcggccag ataaccacaa acctgacgac ccacagcagc
    32881 cagcgaacga caaacgccac acggaatgtc agtgcatcac ccgccatttc cgccctcctt
    32941 gccggcatcc ccgcccagcg ggttcttgtc tccggacata aactgctgcg gattcagatt
    33001 gcccagctgt ttcagcatct ggtcggtgtt aaaaccgttc aggtggcttt ctgccgaggg
    33061 aggattcggc accgggcttt ccgggttacc ctcttccgct gtactctgtg actgctccag
    33121 tgccatttcc tccacggttt tcagctggaa gccgtcctgg aacaccacgg tcacttcgtt
    33181 gcccgcacca atcggtatca ccgggtgata ctgttcggca cgtttgatgt agtagtcact
    33241 gagcgtctgt gcggctttcg atgcgccgcc accgataccc attttcagga catcaccagc
    33301 cccgtaagcg gctgtggcac ccagcccgac agccggttgg gaggcgcgct ccattccctg
    33361 accgataccg tcaacaaatc ccgcgcccca tgcccagccg aggattttgc cgttacgcat
    33421 caccacttcg cccttgatac cgtttttacc ccggaagctg acatgcccct taatcggcat
    33481 atcaatagtt ttgccgtcct tcaggcaact gatattgcgg gtgcgtacaa tggcacgttc
    33541 actggacaca tccccccagg cttccagccc cacaaaacat cccgttgcgt catacgtctt
    33601 gctgttcggc atttccacca ggccggtgat acgcaactgc atcggcaccg tggattcatt
    33661 accggtgact gaggcattgg catccgcccc ttcaatcagc atggctttcg caaaacttcc
    33721 tgacggaatg tacggcagcg atggtccctg ttttccctca ttacgggtaa acaccttacg
    33781 ctgtatccgg ttaggcaccg gcacagactg gtacgtcacc tgtggtggtg gcgtgacacc
    33841 attcccggga taaaacgccg tcggcggtgg aacagcaacg ctgccctgcg gcgggaagga
    33901 tacgggggtg tttcctggct gtggttcgcc ttccggcccg ggcggtgggg caggcatctg
    33961 cggtacaggc tcaccggtcg ccgtgacggg attagcaccc agggctttta cctgctctgc
    34021 cagggcggca ttgtcctgtc ccagcttttc aatacgacgc tggtcatcac cgcgctgttt
    34081 gttcagaaca tccagctcac gacggatttc ctcatactgc ttctgcatct gcgctgccgt
    34141 cacctgcatc tctgtggtgg catgctgacg caccttgtca tcaaaggtcg tatccaccac
    34201 accggtcata tccggcacag gctcctgttc agccacggtt tcaccgttac cggacatgtc
    34261 cacatcagac agatacagtg ccccaccaat cgcggaggct gtaccgacaa ccacaatccc
    34321 cagccacagg tactgcttgc gtttcacaat ggtattgata ctggccatca gttgccctcc
    34381 ccgtcacgga tgacatacac atccatgcgc gcacctgcca gtaactggct ggcaggctgg
    34441 ctgaacatca cggcacgggt tcccggctgc cagaaatcac tttcccggat attcagggca
    34501 gacagcgttt tattttccac gacaaagcgg accatcttca ggtgattccc cgtccatacg
    34561 gaatctgcca ctgaagacag ccccgccggg gcctgcaggg tttcctttgt cacaggaacc
    34621 tgataccagc ctgcaggtaa ttttccgcca cggacagcct ggctgatggt cacaagcagg
    34681 gattcgtaag gcgtggacgt ttcccacgct ccggcttctt ctccggttcc gcgcaggtca
    34741 ctgaccagct gaatggtacg ccccgcgcct tcacggggaa cagcctgaat ggaaaggttc
    34801 agaccacgct ctgtttccag aatgaacgta aagggctttt tgttgacggt ggccaccacc
    34861 acaccgccgg aggcggtctg ctcattattg gtcagggcac catccagact gttcacggcg
    34921 ataatccggt caccgggaac ggtaaacaga ttcgggctgg tattgctgat ggcaatactg
    34981 gcctgaccac cgttcaccat tggcaccaca gcgggggcca gcgtaccgtt tgcggccatc
    35041 acgctgcagg aaaaaaacag gccgccgaat attattgccg tattattttt tctcatcgtc
    35101 tgtttccccg aaattatcca gccaggtcac cccgttttcc cgcttcagaa taaggatgta
    35161 atgtttgata tcagtaaaag gttcggagtt accaatccat gttttccgaa ttccacggat
    35221 ttccacacga ccatactgag gccagacacg gacactggtc tggaaaaaag cagaattcac
    35281 gttatctttt ttaatgagtt tcgcttcttc agccagaata actttcatct ggttctgtgc
    35341 acccgggcgg atatattgca gaagcgcctg atgtgaagca tcgacgattt ccggtgagac
    35401 attcagacgg agggcaataa atgacagcgc catctgctgc aaataagagg catcggcact
    35461 gttctgtgac acggcaaagg gcacattaaa tcccattggg gtcacagcag tgcgttgctc
    35521 attctgaaga cggtagttat tcaccccctg aatgaggtta acagagaggc tgagaacaat
    35581 aagcactgac ataaatataa aggcgatggc cattacacga ctggtactta aacgggcacc
    35641 gtgttccata taacaatcct ggtatcagtt ctatttaatc cactgccgga aacacgaatc
    35701 gggaacatta tgaaaaatac cgcgcagcag ggctgttggc atataccagt aaatcaggtc
    35761 acgtaaccag gaactgcccc gccctttttt cagtttttta atcccgaaat aaaccagaac
    35821 cgctgcacca ataccgaaca gatatttcga tgttatgata ccccagccaa tacagattgc
    35881 tgcggggatc agttcatcca gtggcaggcc aaaccagcgg ctctgattgg tcagtgtttc
    35941 cggaaaacga tatttcttaa gtttattctc gtctcccgac atcgttttat ttcctgtcag
    36001 aatccggcaa cagacatacc tactgcaata aatacagaga gtatggcaaa gccgaccagg
    36061 aacttgatgt ttttggtcat catgtacatg accgcaccga ccagcacctc agccagaaca
    36121 atccatttaa cgatactgga gttcttaccg aaggtcgcct taaccgtttc ctgaccttta
    36181 gccatcaggt cgcctttacc agcagccata gctaactcag ggaacaacat catcagaaca
    36241 gcaaccggga tcactgcacg aaccaggcga agcatattca gacgagtgaa tttagaaaaa
    36301 aacgatttct ttttgacggg cgcagaagca ccctgaacac ttaaaacagt attcataagt
    36361 tacctcaatt tcggataaat gaataaaact agagtgtatt aaatgttata tcttcctctt
    36421 tatctgcctc cctgaatatc tccgagttat aaaaatcagg aaacttgatc aaatgatcaa
    36481 taactctgtc tgcagcctca tctgttttac accatccact acgatttttt gctgcaataa
    36541 gtagctgatt agtttcatca tccagacgca ttgtcagata cttaaccagc gacttaccat
    36601 agatatttcc tgccctgtta aactttggat agcggtgaag atgatcttta agtcttatta
    36661 ctgcctcaaa cgatcgactt cttccgcttc tgttacttgc ctcaattaat agactttcca
    36721 catctacagg taattttagt tttaccatct tacctgttgc agaacgtgta ccaaatcttt
    36781 tcaataacac ctcccgctgt ttatcttctg gttaccactt atgtttgcag aataatcaga
    36841 aaaggtaaat agacggaaag tctatatttt attaacactt tattaacgca tatttatgat
    36901 acacatagcc ttctcgtata aataatgaat aaacttttta ttgtagagta agatgattaa
    36961 ctcatcttta ttagaaacag agaagtagtc gcatatcaga gatgcgtgtt tctttgatgt
    37021 ggagatggat atgtttaata ttcttgctat agcattatga ctgattccgg tgctatataa
    37081 gtaaaataca tcatttacat atgatgatac agaagaatca tcactcattc ttctgattat
    37141 atttctgata tcatcactga caatcgtttt ttgattataa ctgtctttat gacgaacatg
    37201 agcagcatca taaaattgcc acagaataat atttctgtgt ccattaagaa atgactggac
    37261 agatattgtc cagaatctgt tctgaataaa aatatcctct acaagattaa gatgcgtatg
    37321 ctcattcctg tatgcttcca tctctgtgac agagatcaat tcataaaaat ctctctgcga
    37381 cagaagccat ttttcagcac tttgatcttg tgtaagaaat gactcatgaa acagcgtatt
    37441 gcaaaaaata aattttccag tttcgtccct gatacaggct ggatttggat aatcctgaat
    37501 aactgccgtc agattttcca gcagatctat ttgacgagca tgtttttgtt gaatacgatc
    37561 catcggatac ataggaacct cctcacaaag gattctatgg acagtcgatg cggggaggag
    37621 agaactccct gcatcggcga ttttcaaatt caaattttaa cgtggcatta accacgtaat
    37681 taatatacta attatctttc gtactgtcac ctttttaaat cataaaaact ctgatatttg
    37741 aacgaagtca aatttctttt attcatcatc attttttgga aaaaatcgtt ccatctcaga
    37801 tgataccttc tccctgatat cttcaaccat attggcatat tcaaactttg gatttccgga
    37861 gacatgagga ctgagagact caatacccaa aattttcgct actgatgatt gtgtttttac
    37921 aacgcattca agaagcaatt tattaaactc agtctgatta aatgcagact ctttacgctc
    37981 catctgagcc tcatatacac gaagccccag ttcaagaagc attgaagctg ttgctgaaaa
    38041 actgacatct ttttcccttg ccccttcctg tcgacgcttc tcaataatcg aatttatttt
    38101 ttcataggca tcattgctga tatacaggtt caccttagcc attagagatt accttttttg
    38161 aacataaaat aaagttaatt tcggaacact aaaattcata atacaaacag ggacgcaccg
    38221 ctagcagcgc ccctagcggt atcctata
//
LOCUS       DAZFFE010000032        37627 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFFE010000032 DAZFFE010000000
VERSION     DAZFFE010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.032"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            339..1709
                     /gene="aroP"
                     /locus_tag="ACHJ69_001869"
     CDS             339..1709
                     /gene="aroP"
                     /locus_tag="ACHJ69_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO5014866.1"
                     /translation="MEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAG
                     PGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVLY
                     VLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIKV
                     IAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLEL
                     VGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFH
                     ELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPVN
                     TILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQGV
                     VTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLVVLGIGYLFKEKTA
                     KAVKAH"
     gene            complement(1752..2606)
                     /gene="ampE"
                     /locus_tag="ACHJ69_001870"
     CDS             complement(1752..2606)
                     /gene="ampE"
                     /locus_tag="ACHJ69_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO5014867.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2603..3154)
                     /gene="ampD"
                     /locus_tag="ACHJ69_001871"
     CDS             complement(2603..3154)
                     /gene="ampD"
                     /locus_tag="ACHJ69_001871"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO5014868.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3242..4135
                     /gene="nadC"
                     /locus_tag="ACHJ69_001872"
     CDS             3242..4135
                     /gene="nadC"
                     /locus_tag="ACHJ69_001872"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO5014869.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4339..4779
                     /gene="ppdD"
                     /locus_tag="ACHJ69_001873"
     CDS             4339..4779
                     /gene="ppdD"
                     /locus_tag="ACHJ69_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO5014870.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4789..6174
                     /gene="gspE"
                     /locus_tag="ACHJ69_001874"
     CDS             4789..6174
                     /gene="gspE"
                     /locus_tag="ACHJ69_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO5014871.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLACRGGEKVVLRLLQQVNQALNVNTLGMQPSQLVDFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6164..7366
                     /gene="hofC"
                     /locus_tag="ACHJ69_001875"
     CDS             6164..7366
                     /gene="hofC"
                     /locus_tag="ACHJ69_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO5014872.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGVAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLASFSGEWGW
                     LLVLFGFLLAIANKLLMRHPTRLIARQKLLLRIPIMGSLMRGQKLTQIFTILTLTQSA
                     GISFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNVGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7401..8444)
                     /gene="guaC"
                     /locus_tag="ACHJ69_001876"
     CDS             complement(7401..8444)
                     /gene="guaC"
                     /locus_tag="ACHJ69_001876"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO5014873.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEKWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8600..8644)
                     /locus_tag="ACHJ69_001877"
     CDS             complement(8600..8644)
                     /locus_tag="ACHJ69_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5014874.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8669..9289
                     /gene="coaE"
                     /locus_tag="ACHJ69_001878"
     CDS             8669..9289
                     /gene="coaE"
                     /locus_tag="ACHJ69_001878"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO5014875.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9289..10032
                     /gene="zapD"
                     /locus_tag="ACHJ69_001879"
     CDS             9289..10032
                     /gene="zapD"
                     /locus_tag="ACHJ69_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO5014876.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            10042..10239
                     /gene="yacG"
                     /locus_tag="ACHJ69_001880"
     CDS             10042..10239
                     /gene="yacG"
                     /locus_tag="ACHJ69_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO5014877.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10327..10725)
                     /gene="mutT"
                     /locus_tag="ACHJ69_001881"
     CDS             complement(10327..10725)
                     /gene="mutT"
                     /locus_tag="ACHJ69_001881"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO5014878.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKVEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRVYAG"
     gene            complement(10785..13490)
                     /gene="secA"
                     /locus_tag="ACHJ69_001882"
     CDS             complement(10785..13490)
                     /gene="secA"
                     /locus_tag="ACHJ69_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO5014879.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13552..14064)
                     /gene="secM"
                     /locus_tag="ACHJ69_001883"
     CDS             complement(13552..14064)
                     /gene="secM"
                     /locus_tag="ACHJ69_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO5014880.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14295..15212)
                     /gene="lpxC"
                     /locus_tag="ACHJ69_001884"
     CDS             complement(14295..15212)
                     /gene="lpxC"
                     /locus_tag="ACHJ69_001884"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO5014881.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15313..16464)
                     /gene="ftsZ"
                     /locus_tag="ACHJ69_001885"
     CDS             complement(15313..16464)
                     /gene="ftsZ"
                     /locus_tag="ACHJ69_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO5014882.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQAQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16525..17787)
                     /gene="ftsA"
                     /locus_tag="ACHJ69_001886"
     CDS             complement(16525..17787)
                     /gene="ftsA"
                     /locus_tag="ACHJ69_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO5014883.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17784..18614)
                     /gene="ftsQ"
                     /locus_tag="ACHJ69_001887"
     CDS             complement(17784..18614)
                     /gene="ftsQ"
                     /locus_tag="ACHJ69_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO5014884.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPGESTQQQNQAQAEQQ
                     "
     gene            complement(18616..19536)
                     /gene="ddlB"
                     /locus_tag="ACHJ69_001888"
     CDS             complement(18616..19536)
                     /gene="ddlB"
                     /locus_tag="ACHJ69_001888"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO5014885.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLNDKQLAEISSLGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPAGTFYDYEAKYLS
                     DETQYFCPAGLEALQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19529..21004)
                     /gene="murC"
                     /locus_tag="ACHJ69_001889"
     CDS             complement(19529..21004)
                     /gene="murC"
                     /locus_tag="ACHJ69_001889"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO5014886.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21058..22125)
                     /gene="murG"
                     /locus_tag="ACHJ69_001890"
     CDS             complement(21058..22125)
                     /gene="murG"
                     /locus_tag="ACHJ69_001890"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO5014887.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQSQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22122..23366)
                     /gene="ftsW"
                     /locus_tag="ACHJ69_001891"
     CDS             complement(22122..23366)
                     /gene="ftsW"
                     /locus_tag="ACHJ69_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO5014888.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23366..24682)
                     /gene="murD"
                     /locus_tag="ACHJ69_001892"
     CDS             complement(23366..24682)
                     /gene="murD"
                     /locus_tag="ACHJ69_001892"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO5014889.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWVNDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLATRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24685..25767)
                     /gene="mraY"
                     /locus_tag="ACHJ69_001893"
     CDS             complement(24685..25767)
                     /gene="mraY"
                     /locus_tag="ACHJ69_001893"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO5014890.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25761..27119)
                     /gene="murF"
                     /locus_tag="ACHJ69_001894"
     CDS             complement(25761..27119)
                     /gene="murF"
                     /locus_tag="ACHJ69_001894"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO5014891.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKNTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIKLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDCVLSVGK
                     QSHAISTASGVGEHFSDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27116..28603)
                     /gene="murE"
                     /locus_tag="ACHJ69_001895"
     CDS             complement(27116..28603)
                     /gene="murE"
                     /locus_tag="ACHJ69_001895"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO5014892.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSAHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TDVNYHDSGATIRFSSSWGDGEIESRLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGYAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(28590..30356)
                     /gene="ftsI"
                     /locus_tag="ACHJ69_001896"
     CDS             complement(28590..30356)
                     /gene="ftsI"
                     /locus_tag="ACHJ69_001896"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO5014893.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMAEYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLTLSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30372..30737)
                     /gene="ftsL"
                     /locus_tag="ACHJ69_001897"
     CDS             complement(30372..30737)
                     /gene="ftsL"
                     /locus_tag="ACHJ69_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO5014894.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30734..31675)
                     /gene="rsmH"
                     /locus_tag="ACHJ69_001898"
     CDS             complement(30734..31675)
                     /gene="rsmH"
                     /locus_tag="ACHJ69_001898"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO5014895.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLHVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31677..32135)
                     /gene="mraZ"
                     /locus_tag="ACHJ69_001899"
     CDS             complement(31677..32135)
                     /gene="mraZ"
                     /locus_tag="ACHJ69_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO5014896.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32737..33741)
                     /gene="cra"
                     /locus_tag="ACHJ69_001900"
     CDS             complement(32737..33741)
                     /gene="cra"
                     /locus_tag="ACHJ69_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO5014897.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33823..33909)
                     /locus_tag="ACHJ69_001901"
     CDS             complement(33823..33909)
                     /locus_tag="ACHJ69_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO5014898.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33921..34412)
                     /gene="ilvN"
                     /locus_tag="ACHJ69_001902"
     CDS             complement(33921..34412)
                     /gene="ilvN"
                     /locus_tag="ACHJ69_001902"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO5014899.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMC"
     gene            complement(34415..36139)
                     /gene="ilvI"
                     /locus_tag="ACHJ69_001903"
     CDS             complement(34415..36139)
                     /gene="ilvI"
                     /locus_tag="ACHJ69_001903"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO5014900.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHLQLKEAVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRTRQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36258..36290
                     /locus_tag="ACHJ69_001904"
                     /pseudo
     CDS             36258..36290
                     /locus_tag="ACHJ69_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36457..37401)
                     /gene="leuO"
                     /locus_tag="ACHJ69_001905"
     CDS             complement(36457..37401)
                     /gene="leuO"
                     /locus_tag="ACHJ69_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO5014901.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     ALGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQTWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9117 a        10452 c         9150 g         8908 t
ORIGIN      
        1 ccttttgtaa gattattgtg ctggtggcta aggcatggtg ttcagcaggc agacaaatcc
       61 gacatatagc tagttaaaac agtatgttat cagacttaaa gtatctatta aatgtgatga
      121 aaaacttaca attgcttttt ttttatccag aaaatcaaca catctacata aagcaggtgc
      181 attctcgcgc atataccatt atcttgtgat agttgaaagt actatcatca taattcagtt
      241 acataaaatg taaagaaacc aatacaacaa acggaattac aaaattacac acgcattgct
      301 gcgtagttca aaaaaacaac acaacacgag gtttcataat ggaaggtcaa cagcacggcg
      361 agcagctaaa gcgcggcctt aaaaaccgcc atattcagct tatcgcgctg ggtggcgcga
      421 tagggaccgg gctattcctg ggtagcgcct ccgtaataca gtccgcaggg ccagggatta
      481 tcctgggtta cgccattgct ggttttatcg cctttctgat catgcgtcag ctaggtgaaa
      541 tggtagtcga agaacctgtc gcaggctcct ttagccactt tgcttataaa tactggggca
      601 gttttgccgg tttcgcctct ggctggaact actgggtact gtacgtttta gttgccatgg
      661 cagagctgac tgccgtgggt aaatacattc agttctggta tccggaaatc ccaacctggg
      721 tttctgccgc cgtgttcttt gtggtgatta acgccatcaa cctgaccaac gttaaagtgt
      781 ttggcgagat ggagttctgg tttgccatta tcaaagttat cgcggtggta gcgatgatca
      841 tcttcggcgg ctggctgcta ttcagcggca acggcggccc gcaggcgagc gttagcaacc
      901 tgtgggatca gggtggtttc ctgccgcacg gcttcaccgg gctggtgatg atgatggcga
      961 ttatcatgtt ctctttcggt ggtctggaac tggtggggat caccgcagca gaagcagata
     1021 acccggagca aagtattcca aaagcgacta accaggttat ctaccgcatc ctgattttct
     1081 atattggttc gttagccgtt ctgctctcgc tgatgccgtg gacccgcgtt actgctgata
     1141 ccagtccgtt tgtactgatc ttccacgaat tgggcgatac ctttgtggct aatgcgctga
     1201 acatcgtggt actgactgct gcgctctccg tgtacaacag ctgcgtatat tgcaacagcc
     1261 gtatgctgtt tggtctggca caacagggca acgcgccaaa agcgctggcg tctgtcgata
     1321 aacgcggcgt accagtcaac accattctgg tgtctgcgct ggttacggca ttgtgcgtgc
     1381 tgattaacta cctagcccca gagtccgctt tcggactgtt aatggcgctg gtggtttctg
     1441 cactggttat taactgggcg atgattagcc tggcacatat gaagttccgt cgcgccaagc
     1501 aggaacaagg cgtggtaact cgcttccctg ctctgcttta tccgctgggt aactggatct
     1561 gcctgctgtt tatggcggcg gtactggtga ttatgctgat gacgccgggg atggcgattt
     1621 cggtttacct gatcccggta tggctggttg tgttaggtat cggctatctg tttaaagaga
     1681 aaacggcaaa agcagtaaaa gcgcattaat ctctctacgc cctcacccgt gcagggtgag
     1741 ggcaataatc tttacaccaa cgccccgtaa atcgtcagta gcgcaattac caccacgacc
     1801 acgaacgagg ttttcttcgc catggaaacc gctgccttcg gcgtctccac cttatcgaca
     1861 tgcggttcac gcgccagaga gaactgtgcc agacgcgtta atacctgata ttgcgaagta
     1921 tggaaatcac ccagcgaagc aaaccaggcc ggtaacgctt tctccccatg accgatcaag
     1981 gcatatacca cacccgcaag acgaaccggc acccaatcca gtacatgaag cacggcatca
     2041 atgccggact gtaaacgatg atgcggcgtc tggtaacgcg ccagccagta ttgccatgca
     2101 cgcaaaaacg cataccccat cagcgtaacg ggtccccagg ttccccccac aatcagccag
     2161 aacagcggtg caagataaaa acgaaagtta atccacagca atgcattttg cagctcacgc
     2221 aaatactcac gttcatcgca gcctgccggg acgccgtgaa tcatggtgag ttcgccagcc
     2281 atcgtggcac gggcatggct atcattacgt gaagcagctg tcagataagc atgataatga
     2341 agacgaactt tacctgcgcc aatacacagc aaaccaatca gcagccacac cagcagcgtg
     2401 ggaacgttga acaatactcc ctgcaatgcg cgtaacagta aaaaagtcac gcccatcgca
     2461 ataatggtca tgcctaatgt gcgcccgaga gaaaaatgtt tcacccgccg aaagaacgct
     2521 tcaagacgat gatcaagctg ccagtgctcg cccaacttaa acaggcgctc gaaaattaac
     2581 accagtaagg ttgtaaatag cgtcatgttg tctccttgct gaccagtgca cgaaaccgtg
     2641 cccaatcaaa tgcagggccg ggatcggttt tccgatccgg cgcaatatca caatggcccg
     2701 tcatgttatt agcgatatct ggatagcgat caatcagtgc ccgcgtaacc gccgcaagct
     2761 gttgatactg cgcatcggta tacgccagcg tatcggtgcc ttccagctca atcccaatag
     2821 aaaaatcatt gcagcgttca cgcccctgat actgagagac tcccgcatgc caggcacgtt
     2881 tatcgaaagg aacatactgg actatttcac catcacggcg aatcaaacag tgagcggaga
     2941 cgcgcaaatg ggcgatctca gcaaagaaag gatgcgcctg cggatcaata gttccagtga
     3001 ataatgcgtc gatccacgga ccgccaaact cgcctggcgg caggctaata ttgtgcacca
     3061 ccagcagggt gggtgtttcg tcatccgggc ggcaatcgta atgtggtgag ggaacgcggc
     3121 gcgcgccaac cagccacccc tgttctaaca acatgcagga tctccttata tgtggtgcta
     3181 atacccggtt cagagtagca tgtttctacc ttatgattcg ttagctatct ggagttttaa
     3241 catgccgcct cgccgctata accctgacac ccgacgtgac gagctgctgg aacgcattaa
     3301 tctcgatatc cccggcgcgg tggcccaggc gctacgggaa gatttaggcg gaacagtcga
     3361 tgccaacaat gacattacgg caaaactttt accggaaaac tctcgctctc atgccacagt
     3421 gatcacccgc gagaatggcg tattttgcgg caaacgctgg gttgaagaag tgtttattca
     3481 actggcaggc gacgatgtca ccattatctg gcatgtggat gacggcgatg tcatcaacgc
     3541 caatcaaccc ttgttcgaac ttgaaggccc atctcgcgtg ctgttaacgg gcgaacgcac
     3601 tgcgcttaat tttgtgcaaa ctctttcagg agttgccagt aaggtacgcc actatgtcga
     3661 attactggaa ggcaccaaca cgcagctgtt ggatacgcgt aaaaccttgc ccggcctgcg
     3721 ttcagctctg aaatacgcgg tactttgcgg cggcggagcg aatcaccgcc tggggctttc
     3781 tgatgccttc ctgatcaaag aaaaccatat tattgcctcc ggctcagtgc ggcaggcggt
     3841 cgaaaaagcg tcctggttgc acccggatgc gccagtagaa gtcgaagtag agaatctgga
     3901 agaacttgat gaagctctga aagcaggtgc cgatatcatc atgctggata acttcgaaac
     3961 agaacagatg cgcgaagccg tcaaacgcac caacggcaag gcgctactgg aagtgtctgg
     4021 caacgtcact gacaaaacac tgcgcgaatt tgccgaaacg ggcgtggact ttatctccgt
     4081 tggtgcgcta actaaacacg tacaggcact cgacctttca atgcgttttc gctaattgct
     4141 aacagattta tccgctccat cgcgggcgga taaatctccc cttccccaac tttcttcgta
     4201 acgcctcgca aatttcttgt ctttcgctgc aacgaaataa aaaacctcgg aagctgcttt
     4261 cccgtttcgt tttttgcccc tcgcttgtgc ttatggcgtt tgccaaagta gcgccaaccc
     4321 aatcaaggag cgaaacagat ggacaagcaa cgcggtttta cacttatcga actgatggtg
     4381 gttattggca tcattgccat tttaagcgcc attggtattc ccgcttatca aaactacctg
     4441 cgcaaagccg cactcaccga catgctacaa acctttgtgc cttaccgtac cgccgtagag
     4501 ttgtgcgcgc tggaacatgg tggattaaat acctgcgacg gtggcagcaa tggcattccc
     4561 tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg caaagggcgt ggtgtcgctg
     4621 accgggcaag aaagtctcaa tggactaagc gtcgtcatga cgccgggttg ggataacgca
     4681 aacggcgtca ccggctggac gcgtaactgc aatattcaaa gtgacagcgc attgcagcaa
     4741 gcctgcgaag atgtcttccg ctttgatgac gccaactaag gagcggcaat gaatattcca
     4801 cagctcactg ccctgtgtct gcgttatcag ggagtcttgc tggatgccag cgaagaggtg
     4861 gttcatgttg cggtagtcga tgcaccttcg catgagctac tggacgcatt gcatttcgct
     4921 accaccaaac gtattgagat cacctgctgg acacgccaac aaatggaagg tcacgccagt
     4981 cgcacacaac agacattgcc cgtagctgtt caggagaagc atcagcccaa agcagagttg
     5041 ctgactcgaa cgttacaatc tgcgctagaa caacgcgcgt ctgatattca tatcgaacca
     5101 gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg tattgcatcc tttaccggac
     5161 gtttcaccgg atgccggagt cgcattaacc gccagattaa aagtgctggg aaacctggat
     5221 attgcggaac atcgcctgcc gcaggacggg caattcactg tcgaactggc aggaaacgcc
     5281 gtctcatttc gtattgcgac cttagcatgt cggggtggtg aaaaggtggt attaaggttg
     5341 ttacagcagg tgaaccaggc actgaatgtt aacacgcttg gaatgcagcc gtcacaactg
     5401 gtggactttg ctcatgcctt gcaacaacca cagggactgg tgctggtaac tggccctaca
     5461 ggcagcggca aaacggtcac gctttatagt gccctgcaaa cgctgaatac cgctgacatt
     5521 aatatttgta gcgtcgaaga tccggttgag atccccatag ccggattaaa ccagacgcaa
     5581 atccattcgc gtgccggact cacctttcag ggcgttttgc gtgcgttatt gcgccaggat
     5641 cctgacgtca tcatgatcgg agagatccgc gatggcgaaa cggcagaaat tgccataaaa
     5701 gccgcgcaaa ccggtcacct ggtgttgtct accctacaca ctaactccac ctgcgaaacg
     5761 ctggtacgtt tacagcaaat gggagtcgcc cgctggatgc tatcatcggc gcttacgctg
     5821 gtaatagccc agcgtctggt acgcaaactt tgcccgcatt gtcgccggca gcaaggggag
     5881 cccatccata ttccagacaa tgtatggcca tcgccgctgc cccactggca ggcacccggt
     5941 tgtgtacatt gctaccacgg tttttatggt cgcacggcct tatttgaagt tctgcccata
     6001 acaccggtca tacgtcagct tatttccgct aataccgacg ttgaatcgct ggaaacgcac
     6061 gcccgacagg cgggtatgcg aacgcttttt gaaaacggct gcctggccgt ggagcaaggc
     6121 ttaaccacct ttgaagagtt aatccgcgta ttggggatgc cgcatggcga gtaagcaact
     6181 ctggcgctgg catggcataa ccggcgacgg caatgcgcaa gatgggatgc tatgggcaga
     6241 gagccgtgct ttgctgctca tggcactaca gcaacagatg gttaccccac taagcctgaa
     6301 gcgaatcgcc atcaattctg cgcagtggcg aggagataaa agcgcggaag tcattcatca
     6361 actggcgacg ctactcaaag ccgggttaac gctttctgaa gggctggctc tgctggcgga
     6421 acagcatccc agtaagcagt ggcaagcgtt gctgcaatca ctggcgcacg atcttgaaca
     6481 gggcgttgcc ttttccaatg ccttattacc ctggtcagag gtatttccgc cgctctatca
     6541 ggcgatgatc cgcacgggtg aactgaccgg taagctggat gaatgctgct ttgaactggc
     6601 gcgtcagcaa aaagcccagc gtcagttgac cgacaaagtg aaatcagcgt tacgttatcc
     6661 cattatcatt ttagcgatgg caatcatggt ggttgtggca atgctgcatt ttgtactgcc
     6721 ggagtttgcc gctatctata agacctttaa cactccgcta ccggcgctaa cgcaggggat
     6781 catgacgctg gcaagtttta gtggcgaatg gggttggttg ctggtgctat tcggctttct
     6841 gctggcgata gccaataagc tgctgatgcg ccacccaacc aggcttatcg cacggcagaa
     6901 attgctgtta cgcatcccga ttatgggttc actgatgcgg ggacaaaaac tcacgcagat
     6961 ctttacgatt ctgacgctga cacaaagtgc aggcatttct tttttacagg gcgttgagag
     7021 cgtcagagaa acaatgcgct gcccgtactg ggtgcaactt ctgacacaaa tccagcacga
     7081 tatcagtaac ggtcacccca tctggctggc gctaaaaaat gtcggtgagt ttagcccgct
     7141 ctgtttgcaa ttagtgagaa caggagaggc atccggctcg ctggatctca tgttagacaa
     7201 cctcgcccat catcatcggg ataacacaat ggcgctggcg gataacctcg cagccttact
     7261 ggaaccggcg ttgctgatca taacgggagg aattatcggt acgctggtgg tggcaatgta
     7321 tctgccaatt ttccatttag gcgatgcgat gagtggaatg ggataacgct ggcgtgttgc
     7381 tccacgccag cgttggtata ttacaggttg ttgaagatgc ggttttcttg ttcctgcaca
     7441 cggataaacg tggtgcgctt ggtcagttct ttcaggcgtg aagccccaac gtatgtacaa
     7501 gctgaacgca ggccgcccaa aatatcacgc gcggtatttt caaccgggcc tcgcagcggc
     7561 agcttaacgg ttttaccttc tgctgcgcga tattccgcaa cgccgccaac gtgacgtttc
     7621 atcgcagact ctgagctcat gccgtagaac agcataaatt tctcgccgtt ctcctcaacg
     7681 atgcgaccgc cgctctcttc gtggcccgcc agcatgccgc caagcattac gaaatcggca
     7741 ccgccgccaa aggctttcgc gacatcgccc ggcgtggtgc agccgccatc gctgacgatc
     7801 attccgccca gaccgtgcgc agcatcggcg cattcgatca ccgcagaaag ttgcggataa
     7861 ccgacgcctg ttttgacgcg agttgtacaa acagaacctg ggccaatgcc aactttaacg
     7921 atatcggcac cggagaggat aagctcctca cacatttcac cagtcactac gttgcctgca
     7981 caaatggttt tggtcggcca cgcttcacgc gctttcgcaa cgaactgcac gaagtgttcg
     8041 gaataaccat tcgccacgtc aatacaaacg aaatttaatg ccgggttcag gtcgagaatc
     8101 tgtttagttt tttcgaaatc cgcatcagac gtgccggtag aaaccatcac atgtttcagc
     8161 acatcagcgg aagaattgtt gataaacgct tgccactttt cgacagaata gtgtttatgc
     8221 acagcagtca gaatatcaaa agaagccagc gcagaggcca tagaaaatgt gcctacggtg
     8281 tccatatttg cggcaataat cggcacgccg gaccaactct gacctgaatg tttgaaggtg
     8341 aattgacgtt ccagttcaac atcggaacgg cttttaagag tggagcgttt agggcggatg
     8401 agaacgtctt taaaacctaa cttcagatct tcttcaatac gcatgtgcgg attcctgggg
     8461 ttaatggcta taagggtaag agcgcaactc cagtgacgtt atcatacgca ctaatcaatg
     8521 cggcgcaaga ctgcgaaatt gtcttttttt acgctacaat cccataaatt tacctggtaa
     8581 atagtgagcg aacgtttcat caatttcgct ttcgtgcgct catttttaac gttttgataa
     8641 tcatgatgaa ttcccgggaa tataaattat gaggtatata gttgccttaa cgggaggcat
     8701 tggcagtggc aagagtaccg ttgccaatgc gtttgctgat ctcggaatta acgtcattga
     8761 tgccgatatt attgcgcgtc aggtggttga accaggtgca cctgcgctac atgccattgc
     8821 tgatcacttt ggcgctaaca tgattgctgc tgatggaaca ttgcagcgcc gggccttgcg
     8881 cgagcggatc ttcgccaacc cggaagagaa aaactggctt aacgccctgc tgcatccgct
     8941 gattcagcaa gagacgcaac accagatcca gcaagctact tccccctatg tactatgggt
     9001 tgtgccattg ctggtagaaa actcactgta taaaaaagcg aatcgagtgc tggtggtgga
     9061 tgtcagccca gaaacgcaac ttaagcgcac catgcagcgc gacgatgtaa ctcgcgagca
     9121 tgtcgaacaa atccttgctg ctcaggcaac gcgcgaagct cgccttgccg tggcagatga
     9181 cgtcattgat aataacggcg caccggatgc tatcgcatcg gatgttgccc gcctgcacgc
     9241 acactatttg cagcttgcgt cgcagtttgt ctcacaggaa aaaccgtaat gcagacccag
     9301 gtcctttttg aacatccact aaatgaaaaa atgcgtacat ggctgcgcat tgagtttttg
     9361 attcagcaac tcaccgttaa tttacccatc gttgaccacg ctggcgcgct gcatttcttc
     9421 cgtaatgtca gcgaattact ggatgttttc gagcgcggcg aagtccgcac tgagctgttg
     9481 aaagaacttg accggcagca acgtaaactc cagacctgga ttggcgtgcc cggcgtggac
     9541 cagagccgta ttgaagcatt aattcagcag ttaaaagcgg cggggagcgt attaatctcc
     9601 gcgccgcgta tcgggcaatt tctacgtgaa gatcgtttga ttgctctggt gcgtcagcga
     9661 ctgagcatcc cgggcggctg ttgcagcttt gatttaccta cattgcacat ttggctgcat
     9721 ctaccccagg cgcagcgcga cagccaggta gaaacctgga ttgccagcct gaacccgctt
     9781 acccaggcac ttaccatggt gctggattta attcgccagt cggccccctt ccgtaaacaa
     9841 accagcctga atggttttta tcaggataac ggtggcgatg ccgacttgct gcgcctgaat
     9901 ctgtcgctcg attcacagct ttatccgcaa atttccggtc ataagagccg ttttgccatt
     9961 cgttttatgc cgctggacag tgaaaacgga caggtaccag aacgtctgga tttcgaactg
    10021 gcctgttgct aaggagtaaa aatgtcagaa actattacgg tgaattgccc aacctgcggg
    10081 aaaacggtgg tgtggggtga aatcagcccg tttcggccat tttgctccaa acgttgtcag
    10141 ctgatcgacc tcggagaatg ggctgctgaa gaaaaacgaa tccccagcag tggcgatctt
    10201 tccgaaagcg atgactggag cgaagagcca aagcagtgac attctatctg atgcgacgct
    10261 ggcctgataa aacgcgccag cgtcgcatca ggcatcatat gcaaatgtcg gatgcagcgt
    10321 gacgccttat ccggcataca cacgcttaag tttcgcaatt accggttcat tggctggcgg
    10381 aaaatcatcg gcattaagac cgaccagcga catccactca ccgggttgcc cttctttacc
    10441 ccacggctcc ccttcccagc tttcgaccag ccagaaccac agagttatat gcctgtccgg
    10501 gaattcatat tccagttttt caaatagcga aaaatgttgt ggggtaatcc cgacttcttc
    10561 ctgaagttca cgcaccaccg cctgttccgg cgtttcgccc atttcaactt taccgccggg
    10621 aaactccagt ttattcgcca tgtgcgcatc tgctgcgcga cgcgttataa agatttcatt
    10681 gttctcgttg cgaataatac ctaccgcaat ttgcagcttt ttcattgtct taaacctata
    10741 aaaaaggcgc agaatcctgc gccttttact tcaactgtta gcttttattg caggcggcca
    10801 tggcactgct tgtatttttt accagaacca cacgggcaag gatcgttacg tcctactttg
    10861 cgttcaccgg tttgcgccgc cagtgcagct gcggctgcag agtcgtcatc ctgatggcta
    10921 agctgctgca tttgcgctaa acgctcggct tccatacgac gctgttgttc cagctcctca
    10981 acctcttcag gcatacgtac ctgaactttg ctcagcgtac tgataacttc atatttcaac
    11041 gactccagca tcgctgcaaa catggagaac gattcacgtt tgtattcctg cttcggatct
    11101 ttctgtgcat agccacgcag gtggataccc tgacgcagat agtccatcgc tgccaggtgc
    11161 tctttccaca gagagtcgag agtttgcagc atgacgcctt tctcgaagtg acgcatcatc
    11221 tcagcaccaa ccacttcttc tttacgctga tacacttcga tggactgcgc cagaatgcgc
    11281 tcacgcagcg tctcttcatg cagttctggt tctttatcca gccactcggc aattggtaaa
    11341 tcgaggtcga aatcgttctt cagacgttcc tgcagccccg gaatatccca catttcttcc
    11401 agcgactgtg gcggaatgta ggcatcaatg gtcgctttga acacatcttc acgaatgctg
    11461 ttgatggttt cgctcacatc gctgacatcc agcagttcgt tacgctggga gtaaatggcg
    11521 cgacgctgat cgttagccac gtcatcatat tccagcagtt gcttacgaat gtcgaagtta
    11581 cggctttcaa ctttacgctg ggcgttggca atcgctttgg tcacccacgg gtgctcaatg
    11641 gcttcgcctg gcttcatacc cagtttacgc atcatgccgg atactcggtc ggaagcaaaa
    11701 atacgcatca gcgcatcttc catcgacagg tagaaacggg aagaaccagc atccccctga
    11761 cgaccagaac gaccgcgcag ctggttatcg atacgacggg attcgtgacg ttcagtaccg
    11821 atgatatgca ggccacctgc tgccagtacc gcatcgtgac gtacctgcca gtcggcttta
    11881 attttttcaa tttgctctgc agtcggattt tccagcgcgg caacttctgc ctgccagcta
    11941 ccaccgagca caatatcggt accacgaccc gccatgttgg tcgcgatagt caccgcagcc
    12001 ggataacctg cctgagcaac aatcgccgct tcgttggcat ggaatttggc gttcaggacg
    12061 ttgtgcttaa taccggcttt ggtcagttca tttgacacca gctccgattt ttcgatggag
    12121 attgtaccca ccagcaccgg ctggcctttc gcagtacgtt ctttgatatc ttcaatgatc
    12181 gcctgaattt tttccgcttc agtcatgtag accaggtccg gcagatcttt acgaatcatt
    12241 ggacggttgg tcggaacaac gacggtatcc agcttgtaga tggagctaaa ttcgaaagct
    12301 tcggtatcag cagtaccggt catccccgcc agtttttcat acagacggaa gtagttctgg
    12361 aaggtgatcg acgccagcgt ctggttttcg ttctggatct gtacaccttc tttcgcttcc
    12421 acagcctgat gcaggccatc ggaccaacga cgtccctgca tggtacgacc ggtgtgttcg
    12481 tcaacgatga taacttcacc atctttaacg atgtagtcga cgtcgcgggt aaacagcgca
    12541 tgagcgcgca gcgccgccgt tacgtggtgc atcagcatga tgttggccgg agagtacaga
    12601 gactcacctt catccatgat gccttcttta accagcagtt cttcaatcag aaccagacca
    12661 cgttcggtca ggttcacctg gcgagatttt tcatccaccg agaagtggcc ttcgccctgg
    12721 aaggtttcgg agtcttcttt ttcctgacgg atcaggtgtg gaataatttt attcacgcgt
    12781 ttatacattt ccgagctgtc ttctgccggg ccggaaatga tcagcggtgt acgcgcttca
    12841 tcgatcagga tggagtccac ttcgtccacc agcgcatagt gcagtttacg ctgtacacgt
    12901 tcttcaggac tgaacgccat gttgtcgcgc aggtagtcaa agccgtattc gttgttcgta
    12961 ccgtaagtga tgtcagctgc gtaagcttca cgctttgccg gtgctggcat gcccggcagg
    13021 ttgataccaa ctgtcaggcc aagaaattca aacagcggac ggttgttttc ggcgtcacgt
    13081 tgcgccaggt agtcgttgac ggtaactacg tgcacgcctt taccggttag tgcgttcagg
    13141 taagcaggca gcgttgcggt cagggtttta ccttcaccgg tacgcatttc ggcgatgcag
    13201 cgttcgttaa gaaccatacc gccgagtaac tgaacgtcga agtgacgcat accaaagacg
    13261 cgcttacttg cctcacgcac cacggcgaaa gcttccggga tcagattttc cagcacttcg
    13321 cctttttcca gacgtgcacg aaactctgcg gttttccctt tcagttcttc gtcggagagt
    13381 ttttccatct ccggttccat ggcattgatg atgttgacca ctttgcgcat ccggcgcagg
    13441 gtgcgatcgt tacgactacc gaaaacttta gttaataatt tgattagcat aataaaatct
    13501 caaacgcccc gcgttgcgga gtcaataaaa tgaagtaacg gtttattgtt gttagctgag
    13561 gcgttgaggg ccagcacgga tgccttgcgc ctggcttatc cagacgggcg tgctgaattt
    13621 tgcttgcggg gtaaaatgcg cataatcaat gcgataaccc ttttcagacg gcgtgccttc
    13681 ctgggtcaac agcgcgctga gcgtatccag taatgcaaga tgttgcgcct gaagaggcaa
    13741 agattcttca gcaacgggca gcgtttgcgg tgccattgcg aaggaaagat gacggattac
    13801 cgtgcgaatg gcatgttgat gccagtaatc aacggaatag ttcgaattcg ggcggcgcgt
    13861 gttggcttcc agcaaggcca attgaccaaa gttaactttg gctgaaggct cgtggttgcg
    13921 ggttgtcgct tttgcgggcg cgtttggttc ggcggcgttg ctgagtgcag gcaaacctaa
    13981 actcgccgcg accatcccta ataagagatg cggccagaag tagcgtttac caaactgtcg
    14041 ccagcgcgtc agtattccac tcacgttatt gccatcccga tcgtattcaa acgtatttag
    14101 cgcacaaatc ttatcattaa agcctgaagt ccacagcacg aatgaagata agttgtgcgt
    14161 aaaagtgctt aagaaaacag cgttcgcacc gttttttata gctcttataa aattcaaaaa
    14221 aaaacgactg gttcacctgg ccggagagag tgccagattt gccagtcgaa ttttatacga
    14281 cagtataaat gtcgttatgc cagtacggct gaaggcgctt tgaaggccaa cggcagttct
    14341 gcgtcgtcct ggaaggtcac atattcccag gcttcctgtt tcgccaggac agcctgcagc
    14401 agtttgttat tcagtgcatg accggattta taagcggtaa atgcaccgat aatattgtga
    14461 ccacacatga acaagtcacc gatcgcatcg agcattttgt gacgcacaaa ttcgtcttca
    14521 aaacgcaggc cgtcttcgtt cagtacgcga taatcgtcaa caacgatggc acaatcgaag
    14581 ctgccgccca ggcacaaacc acgggactgc agatattcga tatcacgcat gaaaccgaac
    14641 gtacgcgcac ggctgatctg gcgcataaat gcatcagcgg agaagttcat cgcatagcgc
    14701 tggttgctgg aatcaatagc cggatggtta aaatcgatgg tgaaatccag agaaaaacca
    14761 ttgtacggct taaattcagc ccacttatcg ccatcttcga cacgaacagt ttctttaatg
    14821 cgaacaaatt tcttggcgca gttcaactcg tcgataccgg cgtcaagcag caggtataca
    14881 aacggagcgg cgctgccgtc catgatcggg atttccggcg cgttaacttc gataacaatg
    14941 ttatcgatgc ccaggcccgc gagagcagca ttgaggtgct ctacggttga aatccgcaca
    15001 tcatgctcgt tgaccagaca cgtacagagc atggtatcac gcacagattt ggcatcggcc
    15061 gggaaatcta ccggtggatt caagtcggtg cgacgataga tgaccccggt gttggccggc
    15121 gcagggcgta acgtcagggt gactttcttg ccggtatgta aaccgacacc cgtcgcctga
    15181 acgatacgtt taagtgtcct ttgtttgatc atcgtattat ctcgccaaat tacctatcca
    15241 accgaagtgt actatacatt cggcgggcca gtttagcaca aagagcctcg aaacccaaat
    15301 tccagtcaat tcttaatcag cttgcttacg caggaatgct gggatatcca gataatccgg
    15361 ctctttcgca gtttgcggtg cattgtcatt cacgacttta gcaaccggct tctgctcctg
    15421 ggtcagcgga gccatcccgt gctgctggta gcgatccatc actggctgct gagcctgctt
    15481 attggtcacc agagtgattt caggacgttt gtccatgccg atacctgtcg caacaacagt
    15541 tacgcgcagc tcgtcattca tatccgggtc aagagaagta ccgataacca cggtcgcgtt
    15601 gtcggaagca aatgcacgga tggtgttacc taccgtttcg aactcatcca gacgcaggtc
    15661 gaagcccgct gtgatgttta ccagtacgcc gcgtgcgcca gacaggtcga tatcttccag
    15721 cagcggagaa gagatagcca tttcagcagc ttcttccgca cggtcttcac cgctcgccac
    15781 gccagaaccc atcattgcgt agcccatttc agacattacg gtgcgtacgt ctgcaaagtc
    15841 cacgttcatc aaacccggac gagtaatcag ttcggcgata ccttgcacag cgcctttcag
    15901 tacatcgttc gctgcgccaa acgcatccag cagggagata ccacggccca gaactttcag
    15961 cagtttgtcg ttcgggatag tgatcagaga gtccacatgc ttggacagtt cagtgatccc
    16021 ctgctccgcg aatgccatac gcttcttgcc ttcaaagttg aaaggcttag tgacgacagc
    16081 aacggtcagg atacccaaat cttttgccac ttcagcgacg actggtgctg cacctgtacc
    16141 ggtaccacca cccatacccg cagcaataaa gaccatgtct gcaccttcca gcgccgcacg
    16201 cagtgcatcg cgatcctcat cagccgcatt gcggccaact tctggattag cgccagctcc
    16261 cagtcctttg gtgataccgc taccgatttg aatcgtctgt ccaaccgctg ttttacgcag
    16321 cgcttgtgca tcggtattta ccgcgaagaa ttcaacacct tcaatgcgct cacgcaccat
    16381 gtgttcaaca gcattaccgc cgccgccgcc gacgccgatg actttaatca ccgcgtcatt
    16441 ggtaagttcc attggttcaa acatagtttc tctccgattt gtgcctgtcg cctgaggccg
    16501 taatcatcgt cggcctcata aaaattaaaa ctcttttcgc agccaactat tgagtcgctt
    16561 gatccacgag ccaactgatg ctgtaacacg tttttctact tcagcttcac cgttaagatg
    16621 tgactctttc ccatagtgaa gcaatcccac cgccgtcgaa taatacggct cctgagcata
    16681 atccgttaaa ccggtaatgt tcagcggcgc gccgatacgc acttgcgtat gaaacacgcg
    16741 ctgagcacag gctgcaagac cttcgatctg cgctgcgcca ccggttaata caatgcctgc
    16801 cgccaggtga tgtttaaccc cttgttggcg aagcttttcc tgcaactgca atatctcttc
    16861 gttgaccaga ttgagcagct cggtatagcg cggctcgatc acctctgcca gggtctgacg
    16921 ttgtagactc cgcggcggac gaccgcctac gctcggcact tccacgctct catcttttcc
    16981 aacgatggag cccagcgcac aaccgtggcg aactttaatc gcttcggcgt cgcttggcgg
    17041 tgtgccaaag gcgtaagcga tatcactggt cacgacattg ccagcataag gaattacctt
    17101 cgtgtggcgc aatgccccac cggtataaac ggcgatatcc attgtaccac caccgatatc
    17161 gacgacgcag acacccagtt cacgttcatc ttccgtcaat accgaataac ttgatgccag
    17221 tccggcaaat atcagttggt caactttcag cccacaacgt tcaaccgctt tgacgatgtt
    17281 tttcgccata tcgttgtgac atgtgatcag gtgcactttt gcctgcatcc gcacgcccga
    17341 aagccctacc ggattcttga tcccttcctg atagtcaatc gcatactctt gcgggatcac
    17401 atgcagcaca cgatgctcat cgcgcacacg caccgatttc gcggtatgga cgacgttttc
    17461 cacatcttct tgcgtcactt cttcttcaga aataggcacc ataccaattt cattctggca
    17521 gctgatgtgc ttaccagaaa gcgccagata taccgaagag atctgacaat ctgccatcaa
    17581 ttctgcctgg tcaatggcgc gttgtacgca cttgaccacg gattcgaggt cgttcacccc
    17641 gcctttatcc ataccacgcg acgggcagct gcccacgcca atgatattga ccataccgtc
    17701 gggcagaact tcccctacta aagcggcaac cttcgcggta ccaatctcca gtcctactac
    17761 cagttttctg tccgtcgcct tgatcattgt tgttctgcct gtgcctgatt ttgttgctga
    17821 gtagattccc ctggcggcaa gggcgcccag cctactgccg ctccagagtc ataacgcaaa
    17881 tcaacgtagc taatccgttt gccatcggtt tgcgcctgct gctgtaaaac cggataaagt
    17941 tctacaaagc gagccaaacg tttcatcgta tcgccccggc caagattgag cttaatatcg
    18001 ttattcagcg tcaactgcca ggaacgtcgc gcggtcatcg ccgcttcctt cagagtaaat
    18061 ctgtcctttg ccagcatctg ccccatttcg cgatagccct gcaacacttc attggcgctg
    18121 ccttccgggc catacagcat tggaagcacc tgcttgctgg tgcgatctgg cggcacgctg
    18181 aaggtgtttc cttccgcgtc taccatatgt tgatcattcc accgcgcaat cggcacatat
    18241 tcaaccagat gaatcttcaa ttcatcaggc cactgctttc tgacgctcac ctgcttaatc
    18301 cacggcaggc gttgttctat ttgcgtctgg atgatgttga catcctgggt cataaaggta
    18361 cctggctcac ccaatgccag gatcgactgc cggatatcgt cattacgcgt gtaatggcgt
    18421 tcaccggtca acaccagctt tgagagcggc aggcgttgcg catcttccat ccagcccaac
    18481 acgacccagc cgctcaccaa cactgtcgtt aaaacggtca gcaggaaaag gatccccgcc
    18541 agacgcgttc cattattgcg gcgagaagaa acctcttctt cgctatttcg cgtgttcaga
    18601 gcagcctgcg acatattagt ccgccagttc cagaattcgt actaccaact gcgagaagct
    18661 catacctgcc tgacgtgccg ccatcggtac cagactgtgg ctggtcatac ccggtgaggt
    18721 attggcttcc agcagataaa actgtccatc gctgtccagc ataacgtcaa tacgtcccca
    18781 tcctttgcaa cctaacgtcg tccatgcttt tagcactaat gcctgcaaat tggcctcttg
    18841 taacgcttcc agacctgcgg ggcagaaata ctgtgtctca tcagagagat acttcgcctc
    18901 ataatcatag aaggttccgg ccggttgaat acgtattgac ggtaaaattt cttcaccgag
    18961 tatcgcaacc gtgaactccg gcccacttag ccatttttca atcaatactt cttcatcgtg
    19021 ctgaaatgcc aatcttaatg catcttgtag agcattttct gctactactt ttgacattcc
    19081 cacactggaa ccttcgcggc tcggcttaac gataaccggc aaacccagag aagaaatttc
    19141 tgctaactgc ttatcgttaa ggcctttttc aaactctgcg cgggttaacg ctacccacgg
    19201 cgcgaccggt aaaccggcac cctgccacag aagtttgctg cgtagtttat ccattgaaag
    19261 cgcagatgcc atcactccgc tgccggtata aggcaagccc atcagctcga gcatgccctg
    19321 cagcgtacca tcttcaccgc cgcgaccgtg tagtgcgata aacactttct gaaaacccat
    19381 cgacttcagt tgcgtcacgt cgacttcttt cgggtcgaca ggatacgcgt caataccgcc
    19441 ttcacgcagc ccggctaaca ccgctgcgcc agaattcaga gaaacttccc gctcagcgga
    19501 ggtcccgccc aacaggaccg cgattttatc agtcatgttg ttcttcctcc ggagtttgcg
    19561 gcttcagttt gatttcagct aaagaacgag caatttttcc gatattacca gccccctgaa
    19621 cgagaatcag gtcgttaccg gttaataccg gtgccagcat ctcggctacc tgcgccgggt
    19681 ccggcaccag aatgggatca attttcccac gtccacgaat tgtgcgacac agcgaacggc
    19741 tgtccgctcc cggaattggc gcttcaccag ccggatacac ttccagcatc aacagggtat
    19801 caacctgcgt cagcacattg gcgaaatcat catacaggtc gcgcgtacgg gtaaaacggt
    19861 gcggctgaaa cagcattacc aggtttttat ccggccagcc tgcgcgcgcc gctttaatag
    19921 tggcgtccac ttccgtcggg tggtggccgt agtcatcgac cagcattgcc gtaccgcttt
    19981 ttccattcac tggctccagc gggaattcac cgaggaaatc gaaacggcgc cccgtcccct
    20041 ggaagctttc cagcgcacgc aaaatagcct cgtcgtcaat tccctcttcc gtagcaaccg
    20101 caaccgcagc tgcggcgttc agcgcgttat gacgacctgg cgcattcagg gtgacgcgca
    20161 tcggctcttt gtcctggcgc agcagcgtaa agtgcccctg cgggccaatc tgctgataat
    20221 cttctacacg cacgtcggca tcttcgctga agccgtaagt cgtggtctga cgtcccacac
    20281 gcggtaacaa ttcgcggatc accggatcat caacacacat caccgcacga ccgtaaaacg
    20341 gcaggttgtg cagaaaatta ataaaagtct gttttaaatt ctcaaagtct ccctggtagg
    20401 tatccatgtg gtcggcttcg atattggtga caatcgccac catcggttgc agatgcagga
    20461 acgatgcatc actctcatct gcttcggcaa tcaggtaccg accatgcccc aaacgcgcat
    20521 gaacccccgc cgcttttacc agcccgccgt taacgaaggt tgggtcgagc cccgcttctg
    20581 cgtagatgct ggaaaccatc gcggtggttg tcgttttgcc gtgtgttccg gcaatggcga
    20641 tgccatgacg aaaacgcatt aactcagcca gcatttcagc acgacggatc accggaatac
    20701 gcgcttcatg agctgcgaca atttccgggt tatcggcaga aatcgcgctg gaaacaacga
    20761 ccacgctggc atcacgtacg ttttccgggc gatggttgaa ataaatcgtc gctcccagat
    20821 tcattaactg ctgcgtgacc ggatttggcg ctaaatcgga accactgatc tgataacctt
    20881 cattggccag aacttcggca ataccgccca taccggcacc accgatgccg acaaaatgta
    20941 tgtgccgaac gcgacgcatt tcgggcacga tggaacgcag ttttgccaat tgttgtgtat
    21001 tcattcttta cgccattaac ttcttaaatt catacgatgc aaaaggcatc gctacaatta
    21061 cgcccgggca gcccggctca cttcatttgc cactcgctcg gtggcatccg gaatggatgc
    21121 agcgcgggcg cgttctgcca tggttaataa ggtttctcgc gaccacccgg ccagggtgtt
    21181 ggcgacagca tccacgctaa gctgtgactg ctcgataatt ttggcagcgc ccgctttttc
    21241 cagcggtagc gcattccagt attgctgacg gtctttatgt tgaaacggca caaacaacgc
    21301 cggtagtcct gccgcggcga tttcactcac cgttaacgca ccggagcggc aaacgacgac
    21361 atccgcccac gcatacgccg ccgccatatc atcaataaat tccgtcactt tatgctgcgg
    21421 ttgccccgct tcggcatacg cctgttcaac ggattgttgc gaacctttgc cgctctgatg
    21481 ccagatagtg actgaatcac ccagtttcgc agcaacctgc ggcattgtct ggttaagaat
    21541 gcgtgcgccc tgggaacccc ccactaccaa cacacgaacc ggaccttcac gtccagccaa
    21601 acgttgctgc ggcaacggca gcgccagcac atcggtacgc accgggttac ccaccacttc
    21661 cgcattagga aaagcacctg gaaacgcctg catcactttg gtagcaatct tcgccagcca
    21721 tttattggtt aagcccgcaa taccgttttg ttcatgaagt acaaccggaa tgcctaacga
    21781 ccacgcggcc agaccacctg gacctgatac atagccgccc ataccgagca ccacgtcagg
    21841 tttgtacgct ttcataatcg cccgcgcctg acgccaggcg ttgaagatac gcagcggcgc
    21901 agctatcagt gcttttatac cttttccacg cagaccagag atacgaatga aatcaatttc
    21961 gatgccatgt tttggcacta agtccgcttc catacggtcg gcagtcccca gccagcgaac
    22021 ttgccaaccc tgagccatta gatggtgcgc aaccgccagc cccgggaata catgtccacc
    22081 ggttccgcct gccatcacca ttaatcgctt cccttgacca ctcatcgtga acctcgtaca
    22141 aacgcctgcg ctttctccag acgcgtttca taatcaatac gcaacagcat catgatggct
    22201 gtcgacataa tcagtaagct cgaaccaccg taactgatca gcggcaatgt cagacctttg
    22261 gtcggtaaca tccccgccgc cgcgcctacg ttaaccagcg cctggaaact aaaccagata
    22321 ccaatagaac aggcgagaaa accggaaaaa cggtggtcaa tttctaatgc tttgcggcca
    22381 atcgacatcg cgcgaaaagc gacgaagaat accattaaaa gtgccagcac cacaccgaca
    22441 taccccagtt cttcgccgat aatggcgaaa ataaagtcag tgtgcgcttc cggcagatac
    22501 tccagttttt gtaccgagtt acctaaacct tgtccccaca gttcaccgcg accaaacgcc
    22561 atcaacgatt gcgttaactg atagccgctg ccaaagggat cttcccacgg gttccagaat
    22621 gcggtaacac ggcggatacg gtacggttcg gcgaggatca gcaacacaac cgctgaaatg
    22681 cccataccga taatggcaat gaactgccac aatttcgctc cagccaggaa caacatcgcc
    22741 agcgtagtca caaacaacac caccaccgta ccaaggtctg gctgtgccag cagtaacact
    22801 gccaacacca gaattacgcc catcggtttc aggaagccgc gcaggttatt acgtacttcg
    22861 tcgcctttac gcaccagata gttggcgata tagcaaaaca gcgacagttt tgtcagctcc
    22921 gcaggctgaa tacgcagcaa accgagatcg atccaacgcg atgccccttt aaccgagcta
    22981 cccactacca gaacgatcat cagcaggatg atagatccga gcagcatcgt ggcactgtag
    23041 cgttgccaga actccatcgg cagacgcagc gtaatgatcg ccagaatgaa tgccaggatc
    23101 agatagacgc catcacgctt cgcgaagaag aacggatcgt tggttaagcg ttgccctatg
    23161 ggcattgacg ccgaggtcac catgataaag ccaatcgccg cgaggccgaa ggtcagccac
    23221 agtaaggtgc gatcgtacat gatcaggctg tcggtatctt tttcccgcga gcccatcacc
    23281 cagcccttta gcgccgtgga gatccagacc agaatactga atcctggcag gcgcggcatt
    23341 ttcaggcgag ggagagataa acgcatcaac ctaactcctt cgccagacgg gcaaactcat
    23401 tgcctcgttg ttcaaagttc ttgaattgat caaggctggc acaggccggg gagagcagaa
    23461 ccatatcgcc cggctgaaca cgcgtagcaa gcaagcgcat cgcctgttcc atagtttcgg
    23521 tttgttctgc cacttccggg cgtagcgccg ccagctgcgc gccgtcccga ccgaaacaat
    23581 acagacgtac gttatcgcca ttcaggtaac gcgccagtgg gctaaagtcc gccgatttac
    23641 catcgccccc cagcaacaaa tgcagcgtgc cgtctacgtg caggccattc agcgccgctt
    23701 ctgtactgcc gacgttggtc gcttttgaat cgttaaccca gcgcacaccg ttatgctcca
    23761 gcacaacttc aaagcgatgc ggcagaccag tgaatgtggt taacgctttc aggctgctgg
    23821 cacgcggtaa ccctgcggca tctgccagcg ccagcgccgc cagcgcattg gtgtagttat
    23881 gctgcccgga aagtttcatt tctttcacgt tcagcacttt ctcgcctttc acccgcagcc
    23941 aggtttcacc ctgctgatga ttcaggtgat agtcgcccat gttgacgcca aagctgacgc
    24001 agcgttcatc cgcaccgcga atcggcattg ttaaggcatc atcagcatta accacgcaaa
    24061 cttttgcgtt ttcgtaaatg cgcagttttg ctgcacgata ctgttgtaaa ccaaacggat
    24121 agcgatccat atgatcttca gtcacgttca gaatggtcgc tgccaccgct tgtaagctgg
    24181 aagtggtttc cagctggaag ctcgacagtt ccagcacgta cagttcgcac tcatcatcca
    24241 gcagcatcaa cgcaggcagg ccaatattgc cacccacacc aacgttaacc cccgccgctt
    24301 tcgccatttc acctactagc gtggtgaccg tgcttttgcc gttagaaccg gtgatcgcca
    24361 caatcggtgc ttgtgcttca cgacagaaca gctcgatatc gccaacgatt tcgattccgg
    24421 catcagcggc agcgcttaag gatggatgcg ccagtgcaat accgggactg gcgacaatca
    24481 gatctgccgc catcagccat tcatcattca gaccgcccgt gtggcgttct acggcttcgg
    24541 gtaatttatc caggccaggc ggtgtcatac gcgtatccat aacgcgcggc gtcacaccgc
    24601 gagcgaggaa aaagtccacg caggaaagcc cggtgaggcc caggccgata atgacgacat
    24661 ttttaccctg gtaatcagcc atgattaacg taccttcagc gttgccagac caatcaggac
    24721 cagcatcagc gaaataatcc agaaacgcac aatgacgcgc ggttccggcc agcctttcag
    24781 ttcatagtgg tgatgaatcg gcgccatgcg gaaaatacgt tgtccgcgca gtttaaagga
    24841 gccgacctgc aggatgacgg aaagcgtttc taccacgaac acgcccccca taatcaccag
    24901 caggaattcc tgacgcagca gtacggcgat aatacctaac gcaccaccta acgccagcga
    24961 acctacatcg cccataaaga cctgcgccgg ataggtgtta aaccacagga agcccagccc
    25021 tgccccgact atcgcggtac agacaatgac cagttccccg gcgtgtcgta gatacggtat
    25081 gtgtaaatag ctggcaaagt tcatgttgcc ggtcgcccac gccaccagcg caaaaccacc
    25141 ggcgacaaat acggtcggca taattgccaa accgtcgaga ccatcggtca ggtttaccgc
    25201 gttgccagta cccacaatga caaagtaagc cagcagaatg tagaacagcc ccagttgcgg
    25261 catcacatct ttaaagaacg gaaccaccag ctgcgttgcg ggcgtgtctt tgccgacaag
    25321 gtacagcgcg aaggcgacac ccagcgcaat caccgacatc cagaaatact tccaacgggc
    25381 aatcaggccc ttggtgtctt tacgcaccac tttgcgataa tcatcaacaa agccaataac
    25441 accgtaacct accagcacca ccagtacgca ccagacgtac ggattggacg ggtaagccca
    25501 cagcagcacg gagatcacaa tcgccgtcag gatcattatc ccgcccatgg tcggtgtacc
    25561 gcgcttgctg aaatgtgatt caggaccgtc gttacgcacc acctgaccaa aggaaagttt
    25621 ttgcaaatga gcaatcatac gcgggcccat ccacaatgag atgaacagcg cggtcagcag
    25681 gctgacgatg gcgcgaaacg tcagatagga aaagacgtta aagccggaat aatatttgac
    25741 caaatgttcg gccagccaaa ctaacatgtc ccattctcct gtaaagcgcg tactacctct
    25801 tccatggcag cactacgtga acccttaact aaaatcgtaa ttacctgttg ctcagcaatc
    25861 agtgatttaa gacgcgcgat aagcgcagtt ttatcggaaa aatgttcgcc aacgccgctg
    25921 gcggtgctga tagcatggct ttgtttaccc acacttaaca cgcagtcaat accagcagct
    25981 tttgccgctt cgcccacctg tacatggcag gcttcgcttt cagcgcccag ttccgccata
    26041 tcgcccacca ccagcacgcg gtagcccggc atttcagcca gaacctgaac agcggcggtc
    26101 attgaaccga cattggcgtt gtaggagtcg tcgagcagca actggttttc tgccagtttt
    26161 atggggaaca gacggcctgg aacagctttc agatttgcca gccccgcttt gatagcatca
    26221 agcgttgcgc ccacggacat ggagagcgca gcggctgcca gcgcattcgc aatattgtga
    26281 cgccccggca acggcagcag aacatcgacg ctacctgttg gggtttgcag ggtaaattcc
    26341 gtaccgtgcg aggttacatg gatattggtg gcggtgaaat cgctgttggc ggcattaggt
    26401 gagaaacgcc acactttgcg tgagccaatt acgctctgcc agttcaacca gtcgttgttg
    26461 tcggcgttca taatggcgat accgttttcc ggcaggccgc taaagatttc acctttcgct
    26521 ttcgcgacac ccgcaagcga gccaaaacct tccagatgcg ccgctgccag gttgttgacc
    26581 agcgccgctt ccgggcgagt cagactcaca gtccaggcaa tttcaccctg atggttcgcg
    26641 ccaagttcaa taactgcgta atcgtattcc ggcgttaagc gcaacagcgt catcggtaca
    26701 ccgatgtcgt tattgagatt gcctgccgta taaagtgtgt tgccgcactg gcttaaaatt
    26761 gccgccgtca tctctttaac ggaggttttg ccggaggaac ctgtcagagc aaccacgcgc
    26821 gccggaactt gctggcgaac ccatgcagcc agttcaccaa acgccagacg cgtattcttg
    26881 acgattaact gcggcagatc aatatccagc ggacggctaa ccagtagtgc gcctgcgccg
    26941 ccagctttcg cctggtcggc aaaatcatga gcatcgaaac gttcgccttt cagggcaaca
    27001 aacaggcagc ccggcgtcag ttttcgcgtg tcagtggtta cagcatcaag ggtaatatct
    27061 gcaccttgca gttcaccgtt gagaatatcg gtaagttggc taagggttac gctaatcatg
    27121 caatcccccc cagcagacgc gccaccgtga cgcgatcgga gtagtccaga cgctgattgc
    27181 caacaatctg gtaatcctca tggcctttgc ccgcaaccag taccacatca ttctctttag
    27241 cctgcataac ggcgcaagtc accgcttcag cacggccttc catcactttg gcatatccgg
    27301 catctaacat tcccgccaga atatcgttga tgatggcacg aggctcttcg gtacgcgggt
    27361 tatcgtccgt caccaccgcc acgtcagcaa actcttcagc aattgcgccc atcagtggac
    27421 gtttgccttt atcacgatcg ccaccgcagc caaagacgca ccacagcttg cccgcacagt
    27481 gcaggcgcgc cgcctgtaag gctttttcca gtgcatccgg cgtatgcgcg taatccacca
    27541 ccaccgtcgg tttgcctggt gcagtgaaca cttccatacg tccgcaaacc ggttgcagac
    27601 gcgcggcggt tttcagcaaa tcagccagcg gatagccgag tgccagcagt gtcgccagcg
    27661 ccagcagcag gttgctgacg ttgaatgcgc ccatcaggcg actttcaatt tccccatcgc
    27721 cccaactcga gctaaagcga atggtcgcac cgctgtcgtg atagttcacg tcggtcgctt
    27781 tcaaccagcg tccgtgacag ttcggattga tatgatcttc cattgatacc gcaaccgcgt
    27841 ccggcagttt tgccagccag cggcggccca cttcatcgtc ggcgttaata atcgcctgac
    27901 cgcaatgatg tgcagaataa agcagccatt tcgcggcttc gtagtgttcc atatcaccat
    27961 gataatcaag gtgatctcgg cttaagttgg taaagaccga cgccgcaaat ttcaatgccg
    28021 ccacacggtg ttgtaccagc ccgtgggatg aaacttccat tgcgcaaaaa gtcgcgccct
    28081 gatccaccag ccccgccagc tcatgctgaa catcgactgc cgaaccggtt gtattttctg
    28141 tcgggatcac tttccccagc aggccgttac caacggtgcc cattaccgcg ctggtttcgc
    28201 caagcagttg gctccactgc gccaacagct gggtagtcgt ggttttgccg ttggtgcccg
    28261 ttacgcccac gagacgtaaa ttgtcagagg gttcatggta aaagcggccc gccagtgcag
    28321 ataaacgctc gttgagctgg ctgagataga tgaccggtac gccgtgcatt tcacggattt
    28381 caccgtcggt cgcctcatct ttcgcctctg caataatggc agccacacct tgcgctatcg
    28441 cctgcgggat atatcgacgc ccgtccgcct gatgacctac tacagctaca aagagatcgc
    28501 ccgccgcagc cacacggctg tcgagtgtca tctctcgcag tgctcgcgaa ggtgcgtctg
    28561 gcacccacgg agcaagaagg tcgcgcaaat tacgatctgc cacctgtacc ctcgccttga
    28621 ttaatcacaa attcattttt atcgcccgtt gtcagcgcat ccggctcgat gttcatggta
    28681 cgcaatacgc cgcccatgat ggcaccaaag accggcgcgg aaacggcgcc gccgtagtat
    28741 ttacccgcct gcggatcgtt gataacaaca accagcgcga agcgcggctg actcgcaggc
    28801 gcaacgcctg cggtataagc aatatattta ttgatgtagc gaccgtccgg cccgaccttt
    28861 ttcgcggtac cggttttaat ggcgatacga tagcctttaa tcgccgcctt cacgccgccg
    28921 ccgcccggta gcgccacgct ttccatcata tgcaccacgg tgcgaacaat ggattccggg
    28981 aagacacgtt caccgggaac cggggggtca actttggtaa tcgacagtgg gcgataaatg
    29041 ccgtagctgc cgatagttgc gtagactcgc gctaactgta acggtgttac cattagcccg
    29101 tagccgaaag agaaggtggc cctctctatg tcagaccacc gttgtttttg aggatataag
    29161 ccactgcgtt ctccgaccaa ccccaaattg gtcgcttttc ccagtccaaa acgtgagtaa
    29221 gtatctacta acgctgagga cggcatcgct aacgccagtt tggaaacacc gacgttactc
    29281 gacttctgta ataccccggt cagggttaat tcgctgtagc gcgccacgtc tttgatttcg
    29341 tgaccgttaa ttcgataagg aacggtattg agtaccgagt tttcccgcac cacgccacgt
    29401 tgcaacgcgg tcatcaccac catcggttta accgttgagc ccggttcaaa cacgtcggtg
    29461 atggtacggt tacgcatcgc ttctttcggc gtgccgctca gattatttgg gttgtatgac
    29521 gggctgttag ccatcgccag cacttcaccg gtgttgacat ccaccagcac ggcgctacca
    29581 gattcagcct tgttaaaggc taccgcgttg ttcagttcgc gataaaccag cgcctgcagg
    29641 cgttcatcaa tactcagcgt caggttgtgc gctgcctggc tgtcagtaga agaaatatct
    29701 tcaattacgc gaccatagcg gtctttacgc acaatgcgct cacccggctg cccggtaagc
    29761 catttatcga aactcttctc gacaccctca atcccttggc tatcgacgtt agtaaagccg
    29821 atgaggtgag cggtcacttc gccggatgga taataacggc gagattcttc acgcagatga
    29881 atccccggca gtttcagttt tttgatgtac tctgccatgt cagggtttac ctgacgcgcc
    29941 agataaataa agcgcccttt cgggttggcg ttaatgcggg ctgaaagctg atccagcgga
    30001 atgttgagtg cgttagccag cgccttccag cgatcaccga cgctgatacc gccagcgtca
    30061 tgcacttctt tcgggtcagc ccaaatcgct ttaaccggca cgctcaccgc taacgggcga
    30121 ccagaacggt cagtaatcat gccgcgggag gtggaaactt gctgaacgcg aagagaacgc
    30181 atgtcgccct ctttcaccag catatccggg gagataactt gtaaccacgc tacgcgtccg
    30241 agcagaaaag ccagcgccag gagaatacag ccgcataaca acgcaaaacg ccaactgata
    30301 aagttggcat gttcttcctg acgttttggt ttctgcgttt tcgccgctgc tttcatgcgt
    30361 cgcgtttatc cttatttttg cactacgata ttttcttgtg acggatcaac atgctgcatt
    30421 tgcagctttt ccgtggctat tctttccacc cggctatggt cgccgagcgc attctcttca
    30481 aggatcaggt tgcgccattc aatgtctaaa gcatctcgct ccagcaccag ttgttcgcgc
    30541 tgagcggtca gtaaacgggt atggtgcgcc gtggttacca cggtcaccgc cgtcaaaata
    30601 atgcaaatga acaggcagag tggcagcttc ccaaatcgca aaagatcgtc accgataacg
    30661 ccaggcaagg catggcgctc gtggcttccc atcgatcctt taactttgct tagagcttct
    30721 gtcactctgc tgatcatgcg ttcgtcctct ctgcaatacg cagaactgaa ctacgggcac
    30781 gagggttctc agccacctct tcttcgcccg gcatcaactt acctagtgct cgcaactgac
    30841 gcccccccag ttttttgagc tgctcttctg tcatcggtaa ccctgccgga acttgcggac
    30901 cgcggctgtt ttcacgcata aaacgtttca caatgcggtc ttcaagtgag tggaagctga
    30961 tgatcgaaag ccgcccaccc ggggccagca catggagcga gctttttagc gcctgctcta
    31021 tctcctccag ttcactgttt acccaaatgc gcaccgcctg gaaggtacgg gtcgcgggat
    31081 gtttaaactt atctttcacc ggcgttgcag cagccacgac ttccgccagt tctttggtgc
    31141 gggtcatcgg ctgttcgcgg ttacgctcga caatggcgcg ggcaatgcgt ttggcaaaac
    31201 gctcttcacc ataggttttc aatacccagg cgatatcggc ttcttctgcg gtttgtagcc
    31261 attcagcggc tgactgccca cgggttgggt ccatacgcat gtccagcgga ccatcgcgca
    31321 taaaggaaaa gccgcgctca gcatcatcaa gttgcggtga agagacgcca agatcgagga
    31381 gaatgccgtc gatcttgccg ataagatcgc gctcggcaac atattcgccc agcgcggaga
    31441 aaggtccgtg gatgatggag aagcgcggat catcaatagt cttcgcaacg gcgatagcct
    31501 gcgggtcgcg atcgatcgcc agcaaacgcc cctcttcgcc aagctgcgag aggatcagac
    31561 gtgagtgacc accgcgacca aaagtcccat caatgtagat gccatcagga cggatattga
    31621 ggccattaac ggcttcatcc agcagcaccg tagtatgttt atagttttcc atcattttat
    31681 agagacaagt cctgcagtcg ctccgataag tctccggtag ccaactgctc tgcgtcgata
    31741 tcttccttga cctgttgatg ccaggttgtt tcatcccaca gctcaaactt gttgaactgt
    31801 ccaaccagca tcacttcttt tgtcagcccg gcatgttgcc gcagtactgg cgcgattaac
    31861 aatcgacctg cgccatccat ctgacattcg ctggcatgac ctaacagtag gcgctgcaca
    31921 cggcgctcaa ccgggttcat gctcgacaga cgcgataatt tttgctcgat aatttcccat
    31981 tcaggcaggg ggtaaagcag caggcacggg tgatgaatgt caatggtgca aaccatttga
    32041 ccggcagcat tctcaagcag ctgttcccga taacgggtag gcactgataa gcgccctttg
    32101 ctgtcgagat tgactaacgt tgctccccgg aacatgccag cctcacccct tattcccact
    32161 ttgccccaca atttcccact gaaaggagtt tacggagcgg aggaaaagct tgtcaagcca
    32221 gtcacaaggc ataccgaggt aaaaaacccg atgtttacgg ctaatatcag cgttgtttat
    32281 tttacgtcca acgtgcgaag caaattaacg ttatgaatag ctgtaagaaa aaatccctca
    32341 tcgtcatcac cgttttaaaa tgactcacta ttttccacag caaatataaa gtgtcagttt
    32401 gcgacgcgag cggcatttta ggacatatct tctctggtta acagtccctg ttgcgtctgt
    32461 gtggcgcttg atgccagcag gtttgcgcaa aaacaaagaa aagtgtttgt taaatcggca
    32521 attcatctct gcatgtaaca aaataatgca aaaacgcgct gtcaatatca tttaattatt
    32581 gtgaatttca gcaggtgagt taattaacgg gcgggtttaa aagcatttat caataatagc
    32641 caggaatagt cttatttact ttaaggatat tgatgtccat tcccgtactc aacgcgccag
    32701 agagaaattc acctggcgcg tacttttgtt cgcggcttag ctacggctga gcacgccgcg
    32761 gcgatagaga ttacgtttaa tgcgcgttaa accaggtttt ggcttacgcg gttcgtccag
    32821 gctcgccagg acaatctcca gcacacgttc tgcaacatcg cggtgacgtt gagccactgc
    32881 cagcaccggg cactgtaaga agtcgagcag ttcgttatcg ccaaaggtgg caattgccag
    32941 gtcagaaggc agtttgccgt cgcgacgtag cgtgacatcc atcacccctt gcaacaacgc
    33001 aaacgacgtt gtgaacagcg cctgcggcat cggatgcgtt tccagccatt tttcgaataa
    33061 ctgggcagct gcctcccgct catagctgtt ggcataaagg aaatgcactt cgcgcggatc
    33121 atctttccag gcagtacgga agccttgttc acgcaggaag ctgacagaaa gctccggcag
    33181 cgcgccaaga taaagcaccg tctcggcggg aaacttacgt aactcttccg ccagcatttc
    33241 ggcatcatcc tgatcggcac caaccacgct ggtgaagtgt tcacgatcga gggcgcggtc
    33301 cagcgcgaca atcgggaacg ggtcgttagc ccagcgttga taaaaaggat gctcaggtgg
    33361 caacgacgtc gaaacaataa tggcatcaac ctgacgctgt aaaaggtgct caatgcagcg
    33421 catttcgttg tcgggctgat cttccgagca ggcaatcagc agttgataac cccgttgccg
    33481 cgcctggcgt tcaagatagt tagcgatgcg ggtatagctg gtgttctcca gatcggggat
    33541 cacaagacca atagaacgtg tgcgtccagc acgaagccca gctgccacgg cgttcgggtg
    33601 ataattgtgc tcacgcacta ctgccatgac tttttcaacg gttttgtcgc tcacacggta
    33661 ttgcttcgct ttgccgttaa taacatagct tgcagtggtc cgcgacactc ccgccagccg
    33721 agcgatttca tccagtttca caattgcccc ttgcgtaaaa tgtaaaaacc atagccattg
    33781 tactggtatg ggttaaattc attaacatct aagcgcagaa aattaactgc ggcaactgct
    33841 tttattccag gttcccactg atttcgcaaa aaaagcccaa cgtgacatgt tgggctgcaa
    33901 attgcgcatt gagatcattc tcaacacatt attttatcgc cgcgtgaaag tccgaccaca
    33961 ccagagcgag caacttccac aattttcgcc acatcgcgaa tcgatgctaa aaatgcatca
    34021 agcttatcgc tggtgcctgc taattgaacg gtataaagcg agggtgtgac atcgataatt
    34081 tgcccacgga atatttccgt attacgtttc acttcgtcac gcccgtaacc gctggcctga
    34141 attttcacca gcatgatttc ccgctcaacg tgcgcgccct gccccaactc gctcacgcgc
    34201 aagacatcga ccagcttgtg taattgcttt tcgatttgct caagcacttt ttcatcgccc
    34261 acggtctgaa tggtcatacg cgataatgtc ggatcgtcgg ttggcgcaac ggtcaggctt
    34321 tcaatgttgt agccacgctg ggaaaaaagg ccaatcacgc gggataacgc gcctgattca
    34381 ttttcgagta agactgataa tatccggcgc ataatcaggt tctctccgtt ttgcttaacc
    34441 acatttcatc cattccgccc ccgcgaatct gcatcgggta gacgtgctca ctgccatcga
    34501 cggtaacatc aacaaacacc aggcgattat tgcgcacctg ttccagcgcc tcgctaagtt
    34561 tgctttccag ttcttgcgga tgagagatct ggatcccgac gtgaccatag gcttccgcca
    34621 gacggacgaa gtctggtaag gactgcatat aagattgtga atgacggccg gaatagatca
    34681 tgtcctgcca ctgcttcacc atccccagat agcggttatt gagattcacc accagtacgg
    34741 gcaactcata ttgcaacgcg gtagacagtt cctggatgtt catctgaata ctgccgtcac
    34801 cagtgacgca aaccacggtt tcttctggca acgccatttt gacgcccagt gccgcaggta
    34861 aaccaaaacc catcgtgccg aggccgccag agttgatcca gcgacgcggt ttgtcgaatg
    34921 gataataaag cgcagcaaac atctggtgct gcccgacatc ggacgtcacg taagcgtccc
    34981 cattagtcaa ccgccaaaga gtctcgatca ccgcctgcgg tttaatcttt tcactgtgag
    35041 tgtcatattt caggcactga cgagtgcgcc actgttcaat ctgctgccac cagtcgcgga
    35101 tctcatccag cggttgatgg gcggattctt gcgacaggag ttcaagcatt tgttctagga
    35161 cctggcgagc atcccccaca atcgggatat cagcagtcac ggttttagaa attgaggttg
    35221 gatcaatatc gatatgcaga acagtggcat ttgggcagta ctttgccaga ttgttcgtcg
    35281 ttcggtcatc aaaacgtacc ccgacggcga aaatcacatc cgcgttatgc atcgtcatat
    35341 tggcttcgta ggtaccgtgc attcccaaca tgcccagtgc ctgacgatgc gttgccggaa
    35401 acgcccccag ccccatcaaa gaggaaacaa cgggcagatt caacgcctcc accgcttctt
    35461 tcaactgcag atggcagccc gccgtgattg ccccaccgcc tacgtagaca accggttttt
    35521 ttgccgccac cagcgtttgc agagcgcgct taatttgccc tttatgcccg gaggtcgtgg
    35581 gattgtaaga acgcatactg accgactccg gccagacata gggtaatttg ttcgccggat
    35641 ttagaatatc tttcggtaaa tcaacgacca cgggtccagg acgaccactt gccgctagcc
    35701 agaaagcctt tttcagcacc tgcggaatgt cttccgtttg cttaaccaga aaactgtgtt
    35761 taaccaccgg gcgagaaatc cccaccatgt cgcactcctg aaaggcatca taacctatca
    35821 acgaggtcgc tacctgcccg gaaaggacaa ctaatggaat ggaatccata taagcggtgg
    35881 caatgccagt aattgcattg gtcgcccctg gacccgacgt taccagcacg acgccgactt
    35941 ccccggtcgc gcgcgccaga ccatcggcca tatgcaccgc cgcctgctca tggcgaacta
    36001 aaacatgatc gataccaccc acggtatgca atgcatcgta aatatcaagg accgcgcctc
    36061 caggataacc gaatacttgt ttaacgccct gatcgataag cgatcggacg accatctcgg
    36121 ctccagacaa catctccatg gcctgcctca ctgtttgacg gaaaaaatgt gtaaaacaca
    36181 ttaaccgtcc gatcttgctt aagcaattgg cagacagaga cataaacacg gggtaataag
    36241 aataaacagg aggaaaggtg aaaagaaacg ataaaatcct ccattactga ggatgaaatt
    36301 gataataaat cggctgaatc ctacaactta cagcaatttc tctatttttt taattaatcc
    36361 taaaatagag agcacaaaca ttcagaataa aattctacat tcaataaaaa atgcagaata
    36421 aaccagacat tgatgtctga cctattctgc attcagttag cgtttgcaaa ttgagactaa
    36481 ttgctcttcc atccactgat ggcctttatc gcgcccggca gcttcatgcc aggagagata
    36541 acaggttctg ctgttttgtt ttaacggcag cggtaatacc tgtaactcta aggattcagc
    36601 gaactcttca gccaaccaac gcggcgcaat agcgaccaga tgcgtttgcg acaccacgct
    36661 aagtacgctc atcattgcca tgccctgata cgcgatactg gcttgcttat ctaccgtgtc
    36721 ataccaggtt tgactaaatg atgcgaaacg atcgagcgaa accgcagcgt gttgttcgtt
    36781 ataaacatca tgcttcagta acgggccttt aattgttgga tgatttttgc tggctaccaa
    36841 caccatttca tctttaaata atggcacact ggtaaattca gggcgatgga agtcttcata
    36901 actaatcaca aactccgttt cctgataacg cagctgatgt tcagtgttct gatttaatga
    36961 agacttgaac ataacatgta tatttggcgc aatctgctca atgtgattat aaatctgcga
    37021 ggtcagaatg ctgtctaacg ggctgcaaac acaaagatga aatacacgtt cactgctcgc
    37081 gggttcaaaa cctgaaccag gcaattcatt ttgtactagt tgcaatgcct gacgaactga
    37141 gccaaaaagt tgaaatgcgc gagcagtcgg ttgaatacca cggccataac gaacaaaaag
    37201 ctcgtcatta aacatcacct tcaggcgtgc aacagcgtta ctgaccgcag gttgcgacat
    37261 tcccagagca tgagcggcac gggtgatgtt ttgctcctgc atcacggcat cgaaaacggt
    37321 taataagttg agatcgacca tgcgtagctg tggcttgctt aactccgccg tctctggatg
    37381 atctgtttgt acctctggca tacttaactc cactgtcaca cttaactccc tttcccttat
    37441 tggaatgcag agaaataatc ctcgagaata tgatttatca tgcatataaa ataagaaaaa
    37501 gcaaaatgag taaaattagg aaaacataaa gatacaggtt ctgataaaaa cagaatccac
    37561 tgccgagcaa agagtcattt actacagcat aataatccat aatgtttttg ttgcgaaaac
    37621 aatctaa
//
LOCUS       DAZFFE010000033        37265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFFE010000033 DAZFFE010000000
VERSION     DAZFFE010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.033"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..687
                     /gene="aslA"
                     /locus_tag="ACHJ69_001906"
     CDS             <1..687
                     /gene="aslA"
                     /locus_tag="ACHJ69_001906"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFO5014902.1"
                     /translation="DCMVEMNDIFANLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHG
                     RTPFRGAKGSTWEGGVRVPTFVYWKGMIQPRKSDGIVDLADLFPTALDLAGHPGAKVA
                     NLVPKTTFIDGVDQTSFFLGTNGQSNRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAY
                     TQSGYQGGFTGTVMQTAGSSVFNLYTDPQESDSIGVRHIPMGVPLQTEMHAYMEILKK
                     YPPRAQIKSD"
     gene            complement(778..2013)
                     /gene="aslB"
                     /locus_tag="ACHJ69_001907"
     CDS             complement(778..2013)
                     /gene="aslB"
                     /locus_tag="ACHJ69_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO5014903.1"
                     /translation="MQQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYHEKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLDFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGVEFIQFIPVVERLADETAAHAGLKLH
                     APGDIQGELTEWSVCPQEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMLQQTIAEMIDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMVDLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(2160..2236)
                     /locus_tag="ACHJ69_001908"
     tRNA            complement(2160..2236)
                     /locus_tag="ACHJ69_001908"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2200..2202),aa:Pro,seq:tgg)
     gene            complement(2279..2365)
                     /locus_tag="ACHJ69_001909"
     tRNA            complement(2279..2365)
                     /locus_tag="ACHJ69_001909"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2329..2331),aa:Leu,seq:cag)
     gene            complement(2386..2461)
                     /locus_tag="ACHJ69_001910"
     tRNA            complement(2386..2461)
                     /locus_tag="ACHJ69_001910"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2426..2428),aa:His,seq:gtg)
     gene            complement(2520..2596)
                     /locus_tag="ACHJ69_001911"
     tRNA            complement(2520..2596)
                     /locus_tag="ACHJ69_001911"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2560..2562),aa:Arg,seq:ccg)
     gene            complement(2699..4084)
                     /gene="yifK"
                     /locus_tag="ACHJ69_001912"
     CDS             complement(2699..4084)
                     /gene="yifK"
                     /locus_tag="ACHJ69_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5014904.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLVVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(4275..5015)
                     /gene="wecG"
                     /locus_tag="ACHJ69_001913"
     CDS             complement(4275..5015)
                     /gene="wecG"
                     /locus_tag="ACHJ69_001913"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO5014905.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTQVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(5018..6370)
                     /gene="wzyE"
                     /locus_tag="ACHJ69_001914"
     CDS             complement(5018..6370)
                     /gene="wzyE"
                     /locus_tag="ACHJ69_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO5014906.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADAPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(6367..7446)
                     /gene="rffT"
                     /locus_tag="ACHJ69_001915"
                     /gene_synonym="wecF"
     CDS             complement(6367..7446)
                     /gene="rffT"
                     /locus_tag="ACHJ69_001915"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO5014907.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSGHAREFMVAGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPKLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(7443..8693)
                     /gene="wzxE"
                     /locus_tag="ACHJ69_001916"
     CDS             complement(7443..8693)
                     /gene="wzxE"
                     /locus_tag="ACHJ69_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO5014908.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAVIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDIIREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8695..9825)
                     /gene="rffA"
                     /locus_tag="ACHJ69_001917"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8695..9825)
                     /gene="rffA"
                     /locus_tag="ACHJ69_001917"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO5014909.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAAERINQQRLALWQNYYDALAPLAEAGR
                     IELPSIPDDCVQNAHMFYIKLRDIDDRSALISFLKEAEIMAVFHYIPLHASPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9830..10504)
                     /gene="rffC"
                     /locus_tag="ACHJ69_001918"
                     /gene_synonym="wecD"
     CDS             complement(9830..10504)
                     /gene="rffC"
                     /locus_tag="ACHJ69_001918"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO5014910.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10482..11363)
                     /gene="rfbA"
                     /locus_tag="ACHJ69_001919"
     CDS             complement(10482..11363)
                     /gene="rfbA"
                     /locus_tag="ACHJ69_001919"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO5014911.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11382..12449)
                     /gene="rffG"
                     /locus_tag="ACHJ69_001920"
     CDS             complement(11382..12449)
                     /gene="rffG"
                     /locus_tag="ACHJ69_001920"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO5014912.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEYQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWTALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     EIWWKQVQDGSYQGERLGLKG"
     gene            complement(12449..13711)
                     /gene="wecC"
                     /locus_tag="ACHJ69_001921"
     CDS             complement(12449..13711)
                     /gene="wecC"
                     /locus_tag="ACHJ69_001921"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO5014913.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAAADKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHELPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13708..14838)
                     /gene="wecB"
                     /locus_tag="ACHJ69_001922"
     CDS             complement(13708..14838)
                     /gene="wecB"
                     /locus_tag="ACHJ69_001922"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO5014914.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLLVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14894..15940)
                     /gene="wzzE"
                     /locus_tag="ACHJ69_001923"
     CDS             complement(14894..15940)
                     /gene="wzzE"
                     /locus_tag="ACHJ69_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO5014915.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15952..17055)
                     /gene="wecA"
                     /locus_tag="ACHJ69_001924"
     CDS             complement(15952..17055)
                     /gene="wecA"
                     /locus_tag="ACHJ69_001924"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO5014916.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(17295..18554)
                     /gene="rho"
                     /locus_tag="ACHJ69_001925"
     CDS             complement(17295..18554)
                     /gene="rho"
                     /locus_tag="ACHJ69_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO5014917.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18639..18740)
                     /gene="rhoL"
                     /locus_tag="ACHJ69_001926"
     CDS             complement(18639..18740)
                     /gene="rhoL"
                     /locus_tag="ACHJ69_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO5014918.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18881..19210)
                     /gene="trxA"
                     /locus_tag="ACHJ69_001927"
     CDS             complement(18881..19210)
                     /gene="trxA"
                     /locus_tag="ACHJ69_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO5014919.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            19341..20606
                     /gene="rhlB"
                     /locus_tag="ACHJ69_001928"
     CDS             19341..20606
                     /gene="rhlB"
                     /locus_tag="ACHJ69_001928"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO5014920.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20742..22226
                     /gene="gppA"
                     /locus_tag="ACHJ69_001929"
     CDS             20742..22226
                     /gene="gppA"
                     /locus_tag="ACHJ69_001929"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO5014921.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFYDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(22273..24294)
                     /gene="rep"
                     /locus_tag="ACHJ69_001930"
     CDS             complement(22273..24294)
                     /gene="rep"
                     /locus_tag="ACHJ69_001930"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO5014922.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPTLKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24381..24662
                     /gene="ppiC"
                     /locus_tag="ACHJ69_001931"
     CDS             24381..24662
                     /gene="ppiC"
                     /locus_tag="ACHJ69_001931"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO5014923.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24708..26183)
                     /gene="ilvC"
                     /locus_tag="ACHJ69_001932"
     CDS             complement(24708..26183)
                     /gene="ilvC"
                     /locus_tag="ACHJ69_001932"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO5014924.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            26333..27223
                     /gene="ilvY"
                     /locus_tag="ACHJ69_001933"
     CDS             26333..27223
                     /gene="ilvY"
                     /locus_tag="ACHJ69_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO5014925.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEDDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSADKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCV
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(27220..28764)
                     /gene="ilvA"
                     /locus_tag="ACHJ69_001934"
     CDS             complement(27220..28764)
                     /gene="ilvA"
                     /locus_tag="ACHJ69_001934"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO5014926.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28767..30617)
                     /gene="ilvD"
                     /locus_tag="ACHJ69_001935"
     CDS             complement(28767..30617)
                     /gene="ilvD"
                     /locus_tag="ACHJ69_001935"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO5014927.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRSNGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGNKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(30682..31611)
                     /gene="ilvE"
                     /locus_tag="ACHJ69_001936"
     CDS             complement(30682..31611)
                     /gene="ilvE"
                     /locus_tag="ACHJ69_001936"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO5014928.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31631..31894)
                     /gene="ilvM"
                     /locus_tag="ACHJ69_001937"
     CDS             complement(31631..31894)
                     /gene="ilvM"
                     /locus_tag="ACHJ69_001937"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO5014929.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31891..33537)
                     /gene="ilvG"
                     /locus_tag="ACHJ69_001938"
     CDS             complement(31891..33537)
                     /gene="ilvG"
                     /locus_tag="ACHJ69_001938"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO5014930.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(33540..33590)
                     /gene="ilvX"
                     /locus_tag="ACHJ69_001939"
     CDS             complement(33540..33590)
                     /gene="ilvX"
                     /locus_tag="ACHJ69_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO5014931.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33677..33775)
                     /gene="ilvL"
                     /locus_tag="ACHJ69_001940"
     CDS             complement(33677..33775)
                     /gene="ilvL"
                     /locus_tag="ACHJ69_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO5014932.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            34128..35648
                     /gene="yifB"
                     /locus_tag="ACHJ69_001941"
     CDS             34128..35648
                     /gene="yifB"
                     /locus_tag="ACHJ69_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO5014933.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALEHPKPTNAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35673..36011)
                     /gene="maoP"
                     /locus_tag="ACHJ69_001942"
     CDS             complement(35673..36011)
                     /gene="maoP"
                     /locus_tag="ACHJ69_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO5014934.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            36130..36969
                     /gene="hdfR"
                     /locus_tag="ACHJ69_001943"
     CDS             36130..36969
                     /gene="hdfR"
                     /locus_tag="ACHJ69_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO5014935.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDVHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFCSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(37065..37140)
                     /locus_tag="ACHJ69_001944"
     tRNA            complement(37065..37140)
                     /locus_tag="ACHJ69_001944"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37105..37107),aa:Trp,seq:cca)
     gene            complement(37149..37225)
                     /locus_tag="ACHJ69_001945"
     tRNA            complement(37149..37225)
                     /locus_tag="ACHJ69_001945"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37189..37191),aa:Asp,seq:gtc)
BASE COUNT         9112 a        10205 c         9386 g         8562 t
ORIGIN      
        1 gactgcatgg tggagatgaa cgatatcttc gccaatctgt ataaagcact ggagaaaaac
       61 ggtcagcttg ataacacgct gattgtgttc acctctgata acggtccgga agccgaagtt
      121 ccgccgcacg gtcgcacgcc gttccgcggt gcgaaaggtt ctacatggga aggcggcgtt
      181 cgtgtgccaa ccttcgttta ctggaaaggc atgatccaac cgcgtaaatc tgacggcatt
      241 gtcgatctgg cggatctctt ccctacggca ctggatctgg cggggcatcc tggggcgaaa
      301 gtagcgaatt tagtaccgaa aaccaccttt atcgatggtg tggaccagac atctttcttc
      361 ctgggaacaa atggtcagtc taaccgtaag gccgagcact acttcctcaa cggtaaactt
      421 tctgctgtgc gtatggatga gttcaagtat cacgtcctga ttcagcaacc ttacgcttat
      481 acccagagcg gatatcaggg tggattcacc ggcacagtaa tgcaaacggc gggatcgtcg
      541 gtgtttaacc tgtacaccga tccgcaggaa agcgactcta tcggcgtgcg ccatatccca
      601 atgggtgtac cgctacagac cgaaatgcat gcgtatatgg agatcctgaa aaaatatcca
      661 ccacgcgcgc agattaaatc tgattaagcc ggcgctatcg ctgagatcta cctttgccgg
      721 atgcgatgct gacgcatccg acaatcaacc gcaggcggcc acaaaatggc tactttttta
      781 cttattcacc accagcaaat gcgcctgcat aatgtcgctg gccggacgac cgtgcgccag
      841 caaatcaacc attgctttaa gatatggcgg tagatggcgg aaataacgct gatacccggc
      901 acacaaataa ttcagcccgg gtttgccgct ggcatcgagc ataaagcggt gtttcgggca
      961 gcctccccag cacgctttta acacgttgca actgcgacac tgtgccggta actgcttgaa
     1021 tttatcttca ccaaatgcct gctgttgcgg ggaatcaatc atttctgcaa tcgtctgctg
     1081 aagcatattc cctagtcgat actgcggata aacatagtga tcgcaggcat aaacgtcacc
     1141 gttgtgctca acaatcaccg agcgcccaca ggttggctga tgatggcaaa ccgcgcccgg
     1201 tgcgccgaca aaattggcga acgcccattc gatattcatt acgaaaatct tgccgacgtc
     1261 gcgcttaatc cagtggtcga aaatcgccac cagaaactca ccgaactctt gcgggcacac
     1321 cgaccattcc gtcagttcgc cctgaatatc gccgggagca tgtagcttaa gtccagcatg
     1381 agcagccgtt tcatcagcca ggcgctcgac caccggaata aattggatga actcaacacc
     1441 tgcatcacac agaaaatcat agacttgcaa aggttgtaac gcactggtgc gattgacgca
     1501 gaccagcacg ttatagtcga catgatgttt ttgcaggagc gtcagagcac gcatcaccag
     1561 cttatgcgtt ggtctgccac ctttggtcac gcgatattga ttgtggatct cagccggacc
     1621 atccagcgat aaccctacaa gaaaatgatt ttctgccaga aatgcacacc attcgtcatc
     1681 gagcagcacg ccgttagtct ggaagctatt acttatcttc ctgccagcac catatttcgc
     1741 ctgtagcgcc acggcacggc ggtaaaaatc cagcccgagt aacgttggtt cgccgccctg
     1801 ccaggtaaaa gccacttcgt tttgggtttc gctggcagcg atatagtgac ggacatacgc
     1861 ttccagcgtg tcatcgtcca tatgcgtgac tggcttttcg tggtaaaggg attgtttttc
     1921 gagataaaaa cagtagtcac agttcagatt acaatcagaa ccgctcggtt tcgccatcac
     1981 atgaaaagca cgcgttggaa cctgttgctg catggtcgct ccttgcctga agtcatcctt
     2041 caataagtta agggcgaaat cataccgtgg cgcaatgcgt tattacgggc gtttattgcg
     2101 aagggaaagt gacgaaatgc acagaaaaca aaaaacccca ccgaagcggg gttcaaaatt
     2161 ggtcggcgag agaggattcg aacctccgac ccactggtcc caaaccagtt gcgctaccaa
     2221 gctgcgctac tcgccgaaat actgcttttt gaatttttag ttcaattctt taaagtcgtg
     2281 gtgcgagggg ggggacttga acccccacgt ccgtaaggac actaacacct gaagctagcg
     2341 cgtctaccaa ttccgccacc ttcgcattgt cacaacttct aataatgggg tggctaatgg
     2401 gattcgaacc cacgacaact ggaatcacaa tccagggctc taccaactga gctatagcca
     2461 ccactacaaa tcttgttacg cggtattact accaccgcag ctcaagcgcc gggactaaat
     2521 ggcgcgcccg acaggattcg aacctgagac ctctgcctcc ggagggcagc gctctatcca
     2581 gctgagctac gggcgcttag cgccgttgcg ggggtggata atacggactt cccacccctc
     2641 tgtctagtcc ctttttaaat aaattgcgcg tttggttacg gtttgtgcgt tttgctgctt
     2701 attcctccag tttatgcgct ttaccgtggc gattaagccc aaaaacttta taaatcgccg
     2761 tcaccaccag cataaagatg atgccgacaa acagcgacat acgcgtatct tcattaaagt
     2821 acatgccgat caaaacgcaa atcaggaatg ccattgttac gtagttggcc caagggaaca
     2881 ggattgagcg gaacggatgg ctggcaattg ctgctttatg cgcacgacga aaacgcagct
     2941 ggcttatcaa tatcacaaac catggcacca tccccggaag cacgctggca ctgtagacgt
     3001 aaacaaacac acgctgcgga ttgggaatga tgtagttcag gcatgagcca attagcagaa
     3061 tagcaataga tactgccaca cccgcaaccg gtacgccgtg acgggaaact ttcgccattg
     3121 ccgccggtaa ctgacggttt ttcgccagtg catagagcat acgtccgcaa ctgtacatgc
     3181 cgctgttaca gccagagagc gcagccgtca gcaccacaaa gttgataatg cccgccgctg
     3241 cggtaatgcc gattttggcg aaagtcagta cgaacgggct accgttgctg cctatttcat
     3301 tccacgggaa gatagtaacg ataacgaaaa tcgcgccaac gtagaaaatc aggattcgcc
     3361 acagcacctt gcctacggca ctgcgcagcg tcacctgcgg attcttcgct tcaccggcag
     3421 taatgccaat cagctccacg ccctggtagg acgccaccac aatacacaat gcggtcagga
     3481 accctttcca gccaccagca aagaaaccgc catgctctgt gagattgcta aaaccaatcg
     3541 actgcccgcc attgccaaag ccaaagaaaa tcacgcccag gccaatgaca atcatcacga
     3601 taatcgtggt gactttgatc atcgcgaacc agaactcgat ttcgccgtac aacctcaccg
     3661 cagccagatt cgccaacgcc accagcgcca ccgcgatcaa tgcgggtatc cactgcgcca
     3721 tctccgggaa ccagaactgg acataaacgc caatggcggt gatttctgag atccccaccg
     3781 ccatccacat aaaccagtaa gaccaggcgg tgagataacc aaagaatggg ctcatataac
     3841 gatgcgcgta aacggcgaac gaaccggtaa ccggttcgag gaacaacatt tcgcccattg
     3901 aacgcatgat gaaaaagacg aacagcccgg cgatgatata ggccaacaat acggatggcc
     3961 cggcccattt cagggtactg gcggccccca taaacaggcc gacgccaatg gtgcccccca
     4021 aggcgatgag ttcgatatgt cgagcttcca gcccacgctg tagctctggt ttgttatctg
     4081 ccataaatcc tcgtgttgtg tttgcatgct ttccggtgtt accggttatc gttatgggta
     4141 catcgagtgt tgcgaatgtt ttcgtaattc aggagaaatg gcaaataaag cattaaaaat
     4201 ttgaatgctt tgtgtaataa aaaagcagac aggcgacgaa gtgaccactt cgtcgcttta
     4261 caaagagagg aaaatcagag gttgccggta tagtgccagc gtaaataacg cagcaaacga
     4321 agctgacgct taatgcggct cggctgcgaa agcaggcggt agagccactc cagccccagc
     4381 gtttgccaga ttttcggtgc gcgttttacg tgaccggtga aaacatcgta ggtcccgcca
     4441 acgcccatat acagcgcatc tggatgcacc agacggcagt cgcgcatgaa gatctcctgc
     4501 tttggcgatc ccatcgcaac ggtgacgatt tgcgctccgc tggcatgaat gcgttcaaac
     4561 agcgcctgac gctgctcggg tttaaaataa ccatcctgac tgccaacgat attcacattc
     4621 cactggttgc gcagtttagc ttcagtttgc gccagcactt caggtttacc gcccacaaga
     4681 aatacctgtg tcccttcttt gcctgcgcgc gccatcagct cttcccagag atcggcccca
     4741 gcaacgcggg aaacctgcgc ctgcgggtac tttttacgta ctgaacgtac aacgcttatg
     4801 ccatccgcat atttaaattc ggcagcgtta attaactccc tgacctcagc gttatcttca
     4861 atggtcagca ttttttcggc attaatggca accagcgttc cctgcttaag ctgcccgtca
     4921 gcgaacagat aatcgagggc gtgctgcata tcacgccaac caatcaactg taagccacgc
     4981 agcgtatagg tcggtgccgt ggtgttgtta ttcattgtta tccttcaacc tgcgtccgga
     5041 gcgatgattt tgttcgttta tgaatgagtc cggcgctttc aaaaagccag tacaacagtt
     5101 ttgcgatcat cagacatgcg ccgaagacca cgataaaaaa gaccacgcgt gagacaaacg
     5161 aatccagccc ttcacgcgcc agcacgatca tattgaagat cgccccgaag cagaaactgt
     5221 gcaatatcgc agccttatag cgattagtct cgcggttgcc cagctcatac agccagtcga
     5281 accatttgat aatcagtcca accacaatcg ccccgagcgg gatgaacaac gcgccgccca
     5341 taacaaccag tgagcctata agcgtaggcg agatcgccag tccggagtgg ttattcagca
     5401 cttcccaggt aaagtagttg gcggagttca gcaccatact cgggcgaccc ggccacagcc
     5461 aggaagggat aaagacatag aaatcgcgga caattggagc caggccctgg aagtcgatgt
     5521 tgtcgtagtt ctgcaacagc aacgccagat tctcccacgg cgagaaagtg tcgcgagtga
     5581 gatagagaaa cgtatagaac gcttcatcgc cgctcacatt cattccatag cgttttagcg
     5641 ccagccagaa catgcccaca atccccagca cgcccgccgc cgccagcatc cacaacgaaa
     5701 tccagccgcg aataatgcca ataaacagga agatagcgaa tgcgatgatg atattggcgc
     5761 gagtgccgcc gacaatcatg taagtcaaca agccaaaggc aaccgtgctg acgaggaaaa
     5821 acagccacgc tttgctatcc tggcgcagaa aataaaccac cagcatcgcc gggatgaaaa
     5881 agtaaaagaa gcgttttaat gccacgccgg agacttcgct ggaaaagatc tgactgtaag
     5941 aattaagccg gaacagcaaa aagccgttgt gcatgaagaa gatgccgacg ctcaccagag
     6001 cgatgcccat caggatcacc cacgtgagat tggtctccac gcggttcatg gtaaacagcg
     6061 gacggcgcgg tgcatcagca acgcgtttgc gtagacgggt tttgtaggtg acatagtaaa
     6121 ccgcgtagaa gcagcctgca gaaagcaacg cctgcaacaa gatttctggc ggcgcgacac
     6181 caacatcaaa gcgaaatacc agcacgctgg tcagcggaaa gccgaagaaa aaggtgagca
     6241 aaaacagcaa tgaaaagaag acattgaagt taaagcgcac acggcgaaac tcaaaccagg
     6301 tcagcgtggc aataaacagc gtgcagagca gccagacaac aaacaggcca ctgaattgca
     6361 gcagactcat gcgacctccc cggcggcaat cgccaacgcc cgctgccagc cttgtagata
     6421 gttagggcta aagaaggcaa tggtgttttt atccaccgac gccaactggc gctgcgcttc
     6481 acgcacaata tcctcgttga gatcatcggt agtaaacagt actggcaaat gctgttccgt
     6541 catatcctgc cagaacggat tttcccggtt aagcacgcaa ggaatgcccg cctgaatcag
     6601 taagcaaagc gtaccaatgc cctgctggcg ggcaaaaata aagtaaccga gatcgcactg
     6661 gcgaagtagc gtcagatagg cgtcaaattc cagtttttcg ctcagaactt gtaggttttc
     6721 ttcgctgaat aactccagtc ccgcctgacg aacttcctcg atgtacgctt cgttattagg
     6781 cggatatccc atcggcacca ccacttttac cgtatcgcca aattgctgat gaacggcgcg
     6841 caaggcagca atatgctcat tgctgcggtc accggagttc cccaccagaa tggtcatttt
     6901 tccttcacgt tgccgatcgt tcgccatcgt attaagagaa gggtccatcc gcgtcgggaa
     6961 gtacagcagt tctccccgca cctttggatg cgttttggca aaaaagctca aatcaccgcg
     7021 ggtggcaaat acacagccga ctctcttttg cgccaggcga cgtagcgggt aaaaaagctt
     7081 atatctcaag ccactggaaa gctcatacag gtctgctccc cagatatgcc agaaaaactg
     7141 gctgggctta atgccaccac tcagcagagc cagccacagt ttgggattga actgaccgtg
     7201 gaaaaaaaat cgctgctgac ggttagcttt tgctttcgcg atgaccgctt ccgccagcga
     7261 ttttttccca gggaaaaatt gcacagaaag cgccggacag ctatcactta agccgtcgtc
     7321 cttgccagca accataaact cgcgcgcgtg cccgctcgtc gcggccagcg cgtcgttgaa
     7381 aaaccgcaga acggttcggt tatggtgagg gatatccgat cccagtacgt gaatcagtac
     7441 agtcatgccc gcctacgcca gagtaaaaac acgccacaac aaagagaaaa atagacgata
     7501 taggttgcca tataggcctg tgctgcgccc agtgcgccat gcgcagggat tagccagtgg
     7561 gcaaatacca tcaataaagt gaactggctg atttccgcca gaatataaaa ccgcagtgac
     7621 gctttggcga tcaccagata accaaagaca taagcgccca cttttaacac atcacccacc
     7681 aactgccagg caaagagatc gcgcatagcg gtaaatttat tcgacaacag cagccagata
     7741 gcaaaatcac gcagcaacca gacggtaaaa ctcgccgccg ccactgccgg taagacgaat
     7801 ttcagcgatt taaccacttc ccggatgata tcgcgctttt ccgttagccg cgacaacgtg
     7861 ggcagcaaat atacgctgaa cgatgccgta ataaattgca ggtaggcatc ggaaatactg
     7921 ctcacccctt gccagatccc cacttcatcc cagctatact gcgccgccag cagtttacgc
     7981 atcatgatgt aagcaacagg caaggtcacc gacgtaatca acgccatgag cgtaaatttg
     8041 ctcaactgcc ctgccagacc gttatcccag ctgggtttca gatagcttaa cgggatgaca
     8101 ccacgtttga tcaacatgat aacagcagga attaccacca gcgcgggaat cagcgccaga
     8161 cccagcaacg ccccttcata accgcccaaa cgataactga cgtagtacgc gagaacgcca
     8221 atcaagctac cgacaatcag agataacgca ttacctgcgg catcgcgaaa gcctttcatc
     8281 aacgccagta acaggttgcc ccaggcaatc cccatttgca ccagcgccac taaacgcacc
     8341 agcccctgat agtcggtatt accaaacaac ccctggctga ttggcgcagc tgccagcaca
     8401 aaaaccagcg ccattagcgt agagaagcca agtaccatcg ctgatgaagt gccgaccacg
     8461 cggcgcagct gttggggatt atcatgatac tgggcaacgt atttggttac gccgttaaag
     8521 atgcctgccc cggcaagcac gccgagcacg gtaatcaact ggcggaaatt tgccgccagc
     8581 ccaagccccg ccggaccaaa tgacactgcc agcaacttac cgaccagtaa cccggcacca
     8641 atcttgacca gtgtactggc cgccgtccac aaggacgctt ttgccaacga catatcagga
     8701 gaagtagttc aacaaggtcg caattaccgt acgctgattg acgggcgaca ggttgtagaa
     8761 caacggcagg cgcagcaagc gctcgctctc tttggtggtg tagcgatctt cgccgtggaa
     8821 ctcaccaaag cgttcccccg cagggcttgc gtgcagcgga atgtaatgga acaccgccat
     8881 gatttccgct tccttcagga agctaatcaa cgcgctccgg tcatcaatat cccgcagctt
     8941 gatgtagaac atatgcgcgt tctgcacgca gtcatccgga atcgacggca gctcgatacg
     9001 cccggcttcc gccagaggtg ctaacgcatc gtagtagttt tgccacagcg ccagacgttg
     9061 ctggttgatg cgttcggctg cttccagttg cgcccacagg tacgcagctt gcagatcgga
     9121 catcaaatag ctggagccaa tatcgcgcca ggtatattta tcgacctgac cacggaagaa
     9181 ctggctgcgg tttgtgcctt tttcacggat gatctcggct cgttcgatca acgctttatc
     9241 gttaatcagc gtcgcgccgc cttcaccacc cgccgtgtag tttttggttt catggaagct
     9301 aaagcagcca atatgaccaa tggttcccag cgcacgccct ttgtaagtgg acatcacgcc
     9361 ctgagcggca tcttccacca caaacagatt atgcttcttc gccaacgcca taatggtgtc
     9421 catttcgcag gccacacccg cgtaatggac cggcacgata acgcgcgttt tgtcggtgat
     9481 cgccgcttca atcagcgttt cgtcgatgtt catggtgtcc gggcgaacat ccacaaagac
     9541 gattttcgcg ccacgcagca caaaggcatt ggcggtggag acaaaggtgt agctcggcat
     9601 gatcacttca tcgccaggct ggatatcgag cagcagcgcc gccatctcca gcgaagcggt
     9661 gcaggatggc gtcagtaaca ctttggcgct gccaaaacgt tgctccagcc actgctggca
     9721 gcgacgggta aaaccaccat cgccgcacag tttgccgcta cccattgccg actgcatata
     9781 gtcgagttcg gttcccacca ccggcggtgc gttaaatgga atcatgtgat cacctgtata
     9841 accagtacgc ggtgctttct acattcgcac cactttgtat gtatcgttta agcgcggcgg
     9901 tgttgcccat ctgggtcgcc acccgcaaag ttgttttacc gcgagcatac gcccagttta
     9961 gcgccgtttg cattagctca gcacctgcac cgcgtccagc cagcaggcca attcgcgcat
    10021 ctgtcgcatt gagttcccgt aaagagacat aaccgcgaat atcgccggac gccgcacgta
    10081 aaatcagaca ttgatgatca aaggtgccgc gcacggcatt ttcaatccac tgtgcataaa
    10141 agcgaccgct ggcgtcaggc gcataccacg gcgcacgaaa acggctttgc gcaaatgcgg
    10201 cgctggctaa ctgacgtaat gcgggaatat cggtctcttg tgccactact gcaccactat
    10261 cactgacatt gttcacgggt agcgccaaat caacttcacc ttctaccagg gagaatccca
    10321 gctgttgcag ggcatccagt tcacccgtat ttgatgccgc aattttggcc tgcacccgtg
    10381 accacggcgc taacgcgtct ggcgtcagga gcggtgcttc agacgtaatg cgcacgatgg
    10441 cgctgttaac accaaagaag gcgttttccc aggttagtgg ctcaatactg gcgcggacgg
    10501 gcacgaagta actccagcag atattggccg tagccagttt tcgctaatga actggcagca
    10561 cgcttcacac cctcgtcatc gagccagccg ttacgccagg caatctcttc caggcaggca
    10621 atcttaaagc cctggcgttt ttctactgtc tgcacaaaag tgctggcctc aatcaggctg
    10681 tcgtgagtgc cagtatccag ccaggcaaat ccgcgcccga gcagttcaac ggtcaggttg
    10741 cccgcctcga ggtacatctg gttgatggag gtaatctcca gttcaccacg ctccgacggc
    10801 ttcacctgct ttgcgtactc cacgacttta ctgtcgtaga aataaagccc ggtcaccgcc
    10861 cagtttgatt tcggctgttt tggtttttct tccagcgaga tagcgcggaa attgtcgtca
    10921 aactccacca cgccaaagcg ttccgggtcc atcacctgat agccaaatac cgtcgcccct
    10981 tccgtgcgcg ccgcaacatg acgcagcttc ggactgaagc cctgtccgaa gaagatgtta
    11041 tcgcccagca ccagacaaga aggttcaccg ttgaggaagg tttcaccgat gataaaggcc
    11101 tgcgccagac cgtccggact gggctgttcg gcgtattcca gctggatacc gaactcacta
    11161 ccatcgccca gcaggcgctg gaaataacct ttatcttccg gcgtagtgat gatgagaatt
    11221 tcgcggatac cggccaacat cagcaccgac agcggatagt aaatcattgg cttatcgtaa
    11281 atcggcaaca gttgcttcga tacgccgcgc gtaatcggat gcaatcgggt gccggaaccg
    11341 cccgccagga taataccttt catgtttgcc tccgctgaaa attagccttt cagacctaaa
    11401 cgctcgccct gatagctgcc gtcctgcacc tgcttccacc agatttcatt agccagatac
    11461 cactgcaccg ttttacgcat tccactttca aaggtttcct gcggcagcca gccaagttca
    11521 cgggcaattt tcgaagcatc aatggcataa cgcagatcat gccccggacg gtcagcgaca
    11581 aaggtgatca ggtcacgata gtgcgccacg ccgtgcggct tattcggagc cagttcttcc
    11641 agcagctcgc aaatggtttc cacaacatcg agattcttac gctcgttgtg accaccaata
    11701 ttataggttt caccgacttt ccctgtggtc gccacgcaat acagcgcgcg ggcgtgatct
    11761 tcaacataca gccagtcacg gatttgctgc ccgttgccat ataccggcag cggtttaccc
    11821 gccagcgcgt tgaggatcat cagcgggatc agtttttccg gaaagtggta aggaccgtag
    11881 ttattcgagc agttggtgat aagcgtcggc aaaccatagg tccgcagcca ggcgcgcacc
    11941 agatggtcgc tgctggcttt tgacgcggaa taagggctgc tcggcgcata cggcgtggtt
    12001 tcggtgaaga aatcatccgt cgagtgcagg tcaccatata cttcgtcggt ggagatatga
    12061 tgaaaacgga acgctgattt tttatcttcc gtcagcgcag tccagtaagc ccgcgccgct
    12121 tcaagcagtg tataagtccc gacaatgttg gtttcaataa acgctgccgg gccgtcaata
    12181 gaacggtcaa catggctttc ggctgccaga tgcatgacac agtctggctg atactcagtg
    12241 aatacgcgcg ccagttctgc ccgatcgcag atatcaactt tctcaaaggc aaagcgctcg
    12301 ctttgcgcga ccggtgccag cgacatcagg tttccagcgt aggtcagctt atcgaccacc
    12361 accaccgcgt cgctcgtttc gttgatgata taacgcacca gcgccgagcc aataaacccg
    12421 gcaccacctg ttatcagaat ctttctcatc agcgccagac tcctttggca tcgacgacat
    12481 actgctgatg gacgttgtcg ccattgataa ctttgaactg actatgatcg accagcatca
    12541 ccagcacatc tgccgttgcc agcgcctcgt caagctgcgc cagagtacaa agcccggtca
    12601 gtttcttcgg caactcgtgg atgttaggct caacaaccag cgtttcgccg ctgtgccact
    12661 gggcgatcag ttcagcgatt tccatcgcag ggctttcacg caggtcatca atattcggtt
    12721 taaacgccag accaaagcag gcgattttca gttcactggc gcgtttatcg gcagcagcca
    12781 gacaatcagc caccgccgct ttcacctgat cgataaccca gaacggtttg tgatcgttca
    12841 cttcgcgcgc ggtacggata agccgcgcct gctgggggtt ctgtgccacg ataaaccacg
    12901 gatcaacagc gatgcagtga ccgcccacgc cagggcctgg ctgaagaata ttgacgcgag
    12961 ggtgacgatt cgccaggcga atcagttccc agacgttaat cccctgatcg gcacaaatca
    13021 gcgacaattc attagcaaaa gcgatattca catcgcggaa gctgttttcg gtgagcttac
    13081 acatttccgc cgtccgcgag ttagtgacga cacactcacc ttcgaggaaa attttgtaca
    13141 gttcgctggc gcgggccgaa caaaccggcg tcataccacc aatcacgcga tcgtttttaa
    13201 tcagctcgac cataacctgg cccggtaata cgcgttccgg gcagtaagca atgttgacgt
    13261 ccgccaactc gcccacctgc tgtgggaaag tgagatctgg acgcatctct gctaaccatt
    13321 ctgccatctt ctcggttgac cccaccggcg aggtggattc aaggatcacc aacgcgcctt
    13381 ttttcagcac tggcgcaatg gagcgagcag ccgattcaac gtaggtcata tctggctcat
    13441 gatcgccttt aaacggcgtg ggtacagcaa tcaaccaggc atccgcttca actggcgtcg
    13501 tgctcgctcg taaaaaaccg ccttctacgg cagtttttac tacgctcgcc aaatcaggct
    13561 cgacgatatg gatttcgcca cgattgatgg tatcaaccgc gtgttggttg atatcgacac
    13621 caatcacctg tttttgcctt gaggcaaacg cagctgctgt aggcagcccg atgtaaccca
    13681 gtccgataac agaaatggtc gcaaaactca tagtgatatc cgattatttt ttaacgcttc
    13741 cagaatgcga gagcatgcct gaccatcacc atacgggtta tgggcgcggc tcatagcttg
    13801 atattcgttt tcgtctttta aaagacgcgt cacttcctcg acaattcgct gcttatccgt
    13861 gccgaccaga cgcaccgtcc ccgctgtcac cgcttccgga cgctcagtgg tatcgcgcat
    13921 caccagcacc ggtttcccca gcgaaggcgc ttcttcctga atgccgcctg agtcggtcaa
    13981 aatcagccag gcgtggttca tcagccagac aaacggtaaa tactcctggg gatcgatcag
    14041 aatgacattt ttcacatgcc ccagaatgcg attgaccggc tctctgacgt tcgggttgag
    14101 atgcaccgga tagacaatct ggatgtcctg gtgcgtggtg gcgatgtctg ccagcgcatg
    14161 gcagatttct tcaaagccac gaccgaagct ctcacgcctg tgaccggtca ccagaatcat
    14221 ctttttatcg ggatcgataa acgggtaatt tgccgccagt tctgaacgca gcgtatcgct
    14281 gctcatcacc tggtcacgca ccaataacag tgcatcaata actgtattac cggtaatgaa
    14341 gattcggcta tccgcaacgt tttcacgcag caagttttgc cgggaagttt cggttggaga
    14401 aaaatgatac atcgccagat gcccggtcaa tgtacggtta gcctcttccg gccacggcga
    14461 atagagatcg cccgtgcgca gaccagcctc aacgtgacca acaggaatac gctgataaaa
    14521 cgccgccagg ctggttgcca gcgtcgtcgt agtatcgccg tgaaccagca cgacatctgg
    14581 tttgaactcg gcaagaatag gttttagccc ttccagaatc cgacaggtta tctctgtaag
    14641 gccctgtcct ggctgcatta tgttgagatc gtagtcaggt acaatggaaa agagtttcag
    14701 cacctgatcg agcatctccc gatgctgcgc agtgacgcaa actttagcct caaaaaaagg
    14761 atcttttgcc aacgcatgca ccaacggcgc catcttgatg gcttccgggc gcgtaccaaa
    14821 tacagtcagt actttcacat cgattctctt cgaataagcg gcgagcgcct ttgcgctcac
    14881 cgcagcagtg ttgctatttc gagcaacggc gggttaatgc gacaccagcc ccgatcagtc
    14941 ccccgacaat gccccacata atcatcagga aggcacgacg tgggctatcg cgttttaccg
    15001 gttcttccgg cgtacgcaaa tagcgatagg tctgaaaacg cggatccagg gttggaccaa
    15061 cattcagggt gtttaacatg gcccgattct gatcatagtc gagatcaaag gccggaccga
    15121 cggcctgtaa attttccagt cgagcctgga gcattggacg cccaagcagg aacatttctg
    15181 aatcaggtaa ttcctcggca ggcacatctg tcgcactgcg cgaaatatta tgctgctcag
    15241 caattttcag cgcctgttca atactgttca tccggcgatc gtagatggct ttcgccacct
    15301 cttcctgacg cttcacctga gctttcatct ggatggtgcg tgccgcccat gcgcctttca
    15361 gctcatcatt cagatggctg gctgcacgct ggctggcaaa agcaacatac tgacgtaaca
    15421 ggttattagc gtcaggcgca gtttcggcaa tcagcttcac gctgtcgttg accgcgcggg
    15481 tgaagtcacc gggaataaac acgatgttgt tgatcatttc atccagcaac gccgcatcgg
    15541 ctttgctatt gcccaccatc cgctgtttgt aatagtcggt ttgcagccag aactctctgc
    15601 gggtatccca cgaggccagt tgcataacaa actctttata ggcttcgtcc atgaccgatg
    15661 gttggtcggc agaagccatg tttgaacgga catccaggtt acgcaaaaat tgctgctgtg
    15721 agtaatatcc ccccagcata ttcaccgttg gacgatcggt aatcgctgtc gagctccact
    15781 cctggcgagc aaaaaaagta tacgccagcg cgattaacgc aaacgccagc cccatgccaa
    15841 taatccacag cttcccagcc cacaaggtac gaaacaaccc acgaatatcc agttcatttt
    15901 cagcgtcttc ggccggtttc ccaggcattg gttgtgtcat cacatcctca tttatttggt
    15961 taaattgggg ctgccaccac gatttctacg cagtctgcgt tttacgcgct taataaagcg
    16021 agcaactttc caggcacgct tgatgcaata tccatagagg aagaatgcta gcaaaaagag
    16081 caccagcatg acccactccg ggacaaaatg agaatattct gccagcacgc caatggaagc
    16141 gagcagtgct gcggcaaggg taatcagcac aaacgcctgg cgggaagtaa acccggcacg
    16201 catgatcaaa tggtgaatat gctgacggtc tggagagaat gggctcatgc ctttacgcag
    16261 gcgacggtac ataatcgcca ccatatccat tagcggaatg gcgattatcc acaaagcggt
    16321 aaccgggctg atgggatgag ttttgccctg ggtcgtttcg agcaggatcc agataacggt
    16381 aaaaccaatc agcgtactgc ccgcatcccc cataaagact ttgtagcggc gacccaggat
    16441 accaaggtta agcatgatgt atggcaggat ggcggcgatc atcgcaaagc accagattgc
    16501 gagactggtt tgcccgtcga accacaaaat cataccgatt gctgcaaacg agacgcagga
    16561 caacccgccc agcaagccat caatgccatc aaccatgttg aacgcattaa tggccgccca
    16621 gacggcaaat agcgtcagga agtaaccaaa cggtccgagc accatctccc aggagccaaa
    16681 gatataaccc aggctactga gataaagttt gccgaacacc atcataacaa tgccaacagc
    16741 ggcctgtatg gtggcacgga tttttacgct gatatcaaaa cggtcatcca gcgcgccaat
    16801 gaaaacaagc acaccagcac aagcgagata gagagatgca tgcggaatat agtaatcgac
    16861 aattccgaac gtgaagcaaa tccctgcgta aaccgaaatc cccccaacga gaggtatcaa
    16921 tccctggtga cgtttgcgga agtttggttt atccactaaa ccgacttttt ttgccacctt
    16981 acgggcaaaa aacagaaaca gtgtcgtgaa taaaaaaata ctgatgagat cagtactcac
    17041 tgtcagtaaa ttcacaatgc atgctctcag agaaaattat tagcagaagt ataaccacga
    17101 agacctttat tcagaaagga aagtccgttc cgaacctacc agcactatgc gaaaattaac
    17161 tacttgatat gcaaattgca taacacacca gcaaattcca ggaattttcc taacatcgcc
    17221 agcgcggcat taagattaca gatataaaag caaaacgcca cgtaaacacg tggcgttttt
    17281 ggcataagac aaatttatga gcgtttcatc atttcgaaga aatcgtcatt ggtcttggtc
    17341 attgccagtt tattaatgag gaattccatt gcatcgattt cgcccatcgg gtgaatgatt
    17401 ttgcgcagga tccacatttt ctgcagttct tcctgagtcg tgagcagctc ttctttacgg
    17461 gtaccggaac ggttgtagtc gatagccggg aagacgcgtt tttcagcgat cttacgagag
    17521 aggtgcagtt ccatgttgcc tgtaccttta aactcttcgt agataacttc gtccatttta
    17581 gaaccggtat cgataagcgc cgtcgcgata atggtcaggc tgccgccctc ttccacgtta
    17641 cgcgccgcac cgaagaagcg tttcggacga tgcagggcgt tggcgtccac accaccggtc
    17701 aacactttac ctgacgccgg aacaacggtg ttgtaagcgc gcgccagacg agtgatggag
    17761 tcgagcagaa tgataacgtc tttcttgtgc tcaaccaggc gtttcgcctt ctcgatcacc
    17821 atttccgcaa cctgaacgtg gcgagatgcg ggttcgtcaa aggtagaagc aacaacttca
    17881 ccttttacca gtcgctgcat ctcggttact tcttccggac gttcgtcgat cagcagaacc
    17941 atcagcacac aatccgggtg gttgtaagca atgctctgag caatgttctg cagcagcatg
    18001 gttttaccgg ctttcggcgg tgccacaatc agaccacgct gaccacgacc gataggtgat
    18061 gccagatcca gtacgcgagc ggttaaatct tccgtagaac cgttaccacg ttccatacgc
    18121 agacgagagt ttgcgtgcag cggggttaag ttctcaaaga ggattttgtt gcgggcgttt
    18181 tcaggtttgt cgaagttaac ttcgttaact ttcagcagcg caaaatagcg ttcaccttct
    18241 ttcggcgggc gaatcttacc agagatggta tcaccagtgc ggaggttgaa acggcggatt
    18301 tggctagggg aaacgtagat gtcatcagga ccggcgaggt aggagctgtc tgcggaacgg
    18361 aggaaaccaa atccatcctg caatatctcc agtacgccat caccaaagat atcttcgcca
    18421 ctctttgcgt gctgcttcag gatggcaaaa ataatgtcct gcttacgcat acgagccagg
    18481 ttttccagcc ccatattttc gccgagagtg atcagctcag aaaccggcgt attctttaat
    18541 tcggtaagat tcatagtggt gtgagttctt aaacttgggg taaatctcga acttaatgtt
    18601 gtgaatggta tggcaggatc atccatgcct gtttacggtc atcgactcat gtcttttcgc
    18661 tgcctggtca caggagagta cgcagaactg aaacgacaag acggattgag tgacgagcca
    18721 gaaatctgtt cacttcgcat ttaagataaa acgggaagcg ttggaaatta caagattcaa
    18781 acttaataag gtatgtttaa tacgaagtca acactaagtt agcatgactc acgccgggcg
    18841 tccagttttt agcgacgggg cacccgaaca tgaaattccc ttacgccagg ttagcgtcga
    18901 ggaactcttt caactgacct ttagacagtg cgcccacttt ggttgccgcc acttcaccgt
    18961 ttttgaacag cagcagagtc gggataccac ggatgccata tttcggcgca gtgccagggt
    19021 tttgatcgat gttcagtttt gcaacggtca gtttgccctg atattcgtca gcgatttcat
    19081 ccagaatcgg ggcgatcatt ttgcacggac cgcaccactc tgcccagaaa tcgacgagga
    19141 tcgccccgtc cgctttgagt acatccgtgt caaaactgtc gtcagtcagg tgaataattt
    19201 tatcgctcat atataactcc acaggaataa gcctggcgtg ttggtttcgt tgttggtgta
    19261 acattaacca actaaaggtt gactttattt caccggatac gctttcgtaa agcaatagta
    19321 agctgatatt ctaccacact atgagcaaaa cacatttaac agaacagaag ttttccgact
    19381 tcgccctgca tccgaaggtt gtagaagccc ttgaaaaaaa agggtttcat aactgcacgc
    19441 ccattcaggc actggccctt ccgctgacgc tggcgggtcg tgacgtagcc gggcaggcgc
    19501 aaaccggtac cgggaaaacg atggcgtttc ttacgtcaac gtttcattat cttctctctc
    19561 atcccgcgat tgccgatcgc aaggtgaatc agccgcgtgc cttaattatg gcaccgacgc
    19621 gtgaacttgc cgtgcagatc cacgccgacg cggaaccgct ggcggaagct accggcctga
    19681 agctgggtct ggcttacggt ggtgatggct acgacaagca gctgaaagtg ctggaaagcg
    19741 gcgttgacat tctgattggc accacgggtc gtttaattga ctacgccaag cagaaccaca
    19801 ttaacctcgg tgccattcag gtggtggtac tggacgaagc cgatcgcatg tacgatctgg
    19861 gctttattaa agatatccgc tggctgttcc gccgtatgcc gcctgcaaac cagcgcctca
    19921 acatgctgtt ctccgccacg ctttcgtacc gggtacgtga actggcgttc gagcagatga
    19981 acaatgccga atatattgaa gtggaaccgg aacagaaaac gggccaccgt attaaagaag
    20041 agcttttcta cccttctaac gaagaaaaaa tgcgtttgct gcaaacgctg atcgaagaag
    20101 agtggccaga cagagcgatt attttcgcca acaccaaaca ccgttgtgaa gagatctggg
    20161 gccacctggc ggcagatggt catcgtgtcg gtttattgac aggcgatgtc gcgcagaaaa
    20221 aacgtctgcg tattcttgat gaatttaccc gtggcgatct ggatattctg gttgccaccg
    20281 acgttgccgc gcgtggtttg catattcctg ctgtgacaca cgtctttaac tacgatttac
    20341 ccgatgactg tgaagattac gttcaccgta ttggtcgtac aggtcgcgca ggcgcaagcg
    20401 gtcactctat tagcctggcg tgtgaagagt atgcattgaa tttgcctgct attgagacct
    20461 atattggtca ctcaattccg gtaagtaaat acaatccgga cgcattgatg accgatctgc
    20521 caaaaccgct gcgcctcacg cgcccgcgca caggcaatgg tccgcgtcgt actggcgctc
    20581 cgcgtaatcg tcgtcgttca ggttaaagaa aaaatgtcat actccaaatc actcaagctg
    20641 tatcaagatg gcaagagaat gaatcccgat gcgcttactg tcgtaagtga ttcaggtgaa
    20701 ggaatgcagc caacgcagag acagattgaa ggatgaagag tatgggttcc acctcgtcgc
    20761 tgtatgcagc cattgatctc ggttcgaata gttttcatat gctggttgtg cgcgaggtgg
    20821 ctggaagcat ccagacgctg acgcgaataa aacgcaaagt gcgtctggct gccggcctga
    20881 atagcgaaaa tgccctgtct aatgaagcaa tggagcgcgg ttggcaatgt ctgcgcctgt
    20941 ttgctgaacg tctgcaagat atccctccct cgcaaattcg cgttgtcgct acggcgacat
    21001 tacgacttgc cgtcaatgcg ggtgatttta ttgccaaagc gcaggaaatc ctcggttgtc
    21061 cggtacaggt gatcagcggt gaagaggaag cgcgtctgat ttatcagggc gttgctcaca
    21121 ccactggcgg tgccgatcag cgcctggtgg tggatatcgg cggagccagt actgaactgg
    21181 taaccggcac gggtgcacaa accacctctt tgttcagcct gtcgatgggc tgcgttacct
    21241 ggctggaacg ctattttgcc gatcgtaatc tggggcagga aaattttgat gctgcagaaa
    21301 aagcggcacg cgaagtgtta cgtccggttg ccgatgaatt acggtatcac ggctggaaag
    21361 tgtgcgttgg cgcttccggc accgtgcagg cattacagga aatcatgatg gcacagggga
    21421 tggatgaacg cattaccctg gaaaagttgc agcaactgaa acaacgcgcc attcattgcg
    21481 gtcggctgga agagctagag attgacgggc tgacgctgga acgtgcgtta gtgttcccga
    21541 gtggtctggc gatcctgata gccattttta ccgaactgaa tattcagtgt atgaccctgg
    21601 cgggcggtgc gctgcgtgaa ggcctggtct acggtatgtt gcatcttacc gtcgagcagg
    21661 atattcgcag ccgtacgctg cgtaatattc agcgccgctt tatgatcgat attgatcagg
    21721 cacagcgcgt agccaaagtt gcggctaact tctacgatca ggtggaaaat gaatggcatc
    21781 ttgaagcaat aagccgcgat ttgctcatca gcgcctgtca gcttcatgaa ataggcctga
    21841 gcgttgactt caaacaagcg ccgcaacacg ctgcttatct ggtacgtaat ctggatcttc
    21901 ccggttttac ccccgcacag aaaaaactgc tggcgacgct actgctcaac cagactaatc
    21961 cggtcgatct ctcatcgctg catcagcaaa atgccgtacc accgcgcgtc gcagaacaac
    22021 tctgccgttt actccgcctg gctatcattt ttgccagccg tcgccgtgac gatctcgtgc
    22081 cagagatgac attacaggct aaccatgaac tgttgacctt gacgcttccg caaggttggc
    22141 taacccaaca tccgctgggt aaagagatta ttgatcagga aagccagtgg cagagctatg
    22201 tccactggcc gctggaagtg cattaatgag taagtgccgg atgcgatgct gacacatctt
    22261 ttccggcctt gattatttcc ctcgttttgc cgccatcatc gctttcagat tcgccagatg
    22321 gctttgccct ttctgcatcc gttcttcggc gctgaccact ttgcgctcct gttcccaaat
    22381 caaatcatcc tgcggcagct ccagcaggaa gcggctcggc tccgggcgca ccagttcgcc
    22441 gtactgacgg cgttctttac acagcgtaaa ggtcaattcc ttctgggcgc gggtaatgcc
    22501 gacataggcc agccgccgct cctcatcgat attatcttca tcgatactgc tctggtgggg
    22561 caaaaatcct tcttccatac caaccatgta gacataggga aactccagtc cttttgacgc
    22621 gtgcaaagtc atcagttgca cctgatccag ctcttcctcg ctctcgccgc gctccatcat
    22681 gtcgcgcaga gtaaagcgcg tcaccacctg ggtgagcgtc atcggctcat ccagttcgct
    22741 gccttccagc atctccgtca tccagctaaa cagttggttg acgttcttca tgcgcatttc
    22801 ggcagctttc gggctgggcg atgtttcgta cagccaggat tcataatcca tgccgtggat
    22861 cagatcgcgc accgcggcaa tcggctcccg ctccgccaga cgctggattt ctgccagcca
    22921 gtgagtgaag cgggtcaatg cttcataacc acgtccgcta agcgtctgac tcaggcccat
    22981 atcaaagctg gcggtaaaca tgcttttatt gcgcgtcatc gcccattcac ctagcttttt
    23041 cagcgtcgcc gggccaatct ctcgcttcgg cgtgttaacg atacgcagaa atgcgctgtc
    23101 atcgtccggg ttagtcagca cgcgcagata agccagcaag tccttgattt caggacgaga
    23161 gaaaaacgac gtaccaccag aaattttgta cgggatgcgg ttttgcatca ggaatttttc
    23221 aaatacccgc gactgatggt tgccgcgata aagaatggcg taatctttgt actgcgtttt
    23281 attgacgaag tgatgggcga tcagctcgcc ggtgacgcgc tcggcttcat gttcctcgtt
    23341 attcgcgctt aaaactttta gctctgtgcc gtaacccagt tcggagaaca gacgcttttc
    23401 aaagacgtgc gggttattgg cgatcaggat gttcgccgct ttcaaaatac gtccggaaga
    23461 gcggtagttc tgctcaagct taatcacctt cagcgtcgga aaatcctggc tcagcagcac
    23521 cagattctgc ggacgtgcgc cgcgccagga gtagatcgac tggtcatcgt cacccaccac
    23581 ggtaaagcgc gcgcggctgc ccaccagcag tttcaccagt tcatactggc tggtgttggt
    23641 gtcctgatac tcatccacca gcaggtagcg aattttgttc tgccagcgct cgcggacttc
    23701 ttcattgcgt tgcagcagca acgtcggcaa taaaatcaga tcatcgaagt cgagaacgtt
    23761 acaggctttc aggtgtgcat catacagccc ataacaatgg gcaaaaatac ggtcccgctc
    23821 gccctttgcc tctgccgccg cctgcgccgg tgttttgaga tcgttcttcc agttggagat
    23881 ggtcgaaatt agctgttgca ggagaacttt gtcatcttca atcagcccct cggtcaactc
    23941 tttaagcaaa gcaagctgat cggtatcgtc aaacagcgaa aagttagcct tcatccccag
    24001 cgccgcatac tcgcgtttga tgatatccag ccccagcgtg tggaaggtgg agatcatcag
    24061 cccacgcgcc tctttgcgcc ccagcgtctg cccgacacgc tctttcatct cgcgcgccgc
    24121 cttattggta aaggtcaccg ccgcgatatg tcgcgcctga taaccgcaac cgcggatcag
    24181 gtgggcgatt ttattggtga taacacgagt tttaccggaa cccgcgcccg ccagcaccag
    24241 gcaggggccg gtaacgaatt cgacagcttg ttgttggccg gggtttagac gcataggaaa
    24301 atcgctcaat gaaagtcggg aggggaaaat aacagcgtgg tagtatagcc agcctcatcc
    24361 ttaccactca aggcacgatc atggcaaaaa cagcagcagc actgcatatc cttgtaaaag
    24421 aagagaaact ggctctggat cttctcgagc agatcaagaa cggtgccgat ttcggcaagc
    24481 tggcgaagaa acactccatt tgcccgtcag gcaaacgcgg cggtgatttg ggtgaattcc
    24541 gccagggtca aatggttccg gcgttcgata aagtggtttt ctcttgtccg gtactggagc
    24601 caaccggccc gctgcatacc cagttcggtt accacatcat taaggtgctg taccgtaact
    24661 aacagcaagg ccttctccgg gagaaggcct tgaactctga atcgctatta acccgcaaca
    24721 gcaatacgtt tcatatccgt catatagccg cgcagtttct tacctacctg ctcaatcgca
    24781 tggctgcgaa tcgcttcatt tacatcacgc agctgcgcgt tatctaccgc accttccgga
    24841 atagctttac ccaggtcgcc cggttgcagc tctgccataa acggtttcag cagtggcaca
    24901 caagcgtaag agaacagata gttaccgtac tcggcagtat cggagataac cacgttcatt
    24961 tcgtacagac gcttacgggc gatggtgttg gcaatcagcg gcagttcgtg cagtgattca
    25021 tagtaagcag attcttcgat gatgccggaa tcaaccatgg tttcaaacgc caactcaacg
    25081 cctgctttta ccatcgcgat catcagtacg cctttatcga agtactcctg ttcaccgatt
    25141 ttgccttcat actgcggcgc ggtttcgaat gcggttttgc cagtctcttc acgccaggtc
    25201 agcagtttct tatcgtcgtt ggcccagtca gccatcatgc cggaggagaa ttcgccggag
    25261 atgatgtcgt ccatatgttt ctggaacagc ggcgccatga tctctttcag ttgctcagaa
    25321 agcgcgtaag cacgcagttt cgccgggtta gaaagacggt ccatcatcag ggtgatgccg
    25381 ccctgtttca gcgcttcggt gatggtttcc caaccgaact gaatcagttt ttctgcgtat
    25441 gccggatcgg tgccttcttc caccagcttg tcgaagcaca gcagagaacc agcctgcaac
    25501 ataccgcaca gaatggtttg ctcacccatc aggtcagatt tcacttccgc aacgaaggac
    25561 gattccagca cgcccgcacg gtgaccgccg gttgcagccg cccatgcttt agcgatcgcc
    25621 atgccttcgc ctttcggatc gttttccggg tgaacggcaa tcagcgtcgg tacgccgaat
    25681 ccacgtttgt actcttcacg tacttcggtg ccagggcatt tcggcgcaac cattacgacg
    25741 gtgatgtctt tacggatctg ctcgcccact tcgacgatgt tgaaaccgtg tgagtaaccc
    25801 agcgccgcgc cgtctttcat cagtggctgt acggtgcgca ctacatcaga gtgctgcttg
    25861 tccggcgtca ggttaaccac cagatccgcc tgcgggatca gttcttcgta agtacccact
    25921 ttaaaaccat tttcggttgc tttacgccag gatgcgcgct tctcagcaat cgcttcttta
    25981 cgcagagcgt aggagatatc gagaccagaa tcacgcatgt tcaggccctg gttcagaccc
    26041 tgtgcgccac agccgacgat gactactttt ttaccctgaa ggtagctcgc gccatcggcg
    26101 aattcatcgc gccccataaa gcgacatttg cccagctgtg ccaactgctg gcgcagattc
    26161 agtgtattga agtagttagc catggtgatt cctcgtgatg ttgtgcttct tattgttcgg
    26221 ttcgcgtttg cgagattgaa ttcactatat gacaggaaat ttattgcgga aattgatata
    26281 ttcacaacgt cacattgcaa tttctgcaac gtcaacatta agggctgcgc cattggattt
    26341 acgcgatctg aaaaccttcc tgcatctggc ggaaagccgc cattttggcc gcagcgcgcg
    26401 ggcaatgcac gttagcccgt ccacgctctc tcggcagatt cagcgcctgg aagacgatct
    26461 cggtcagccg ctgtttgtgc gcgataaccg cacggtaacg ctaaccgaag caggcgaaga
    26521 actgcgcgtt ttcgcccagc aaacgctgtt gcagtatcaa cagctgcgcc acaccatcga
    26581 tcagcaagga ccgtcgctct ctggcgaatt acatatcttc tgctcggtga ccgctgccta
    26641 cagccatctg ccgccgattc tcgatcgctt ccgcgcggaa cacccgtcgg tggagattaa
    26701 actcacgact ggtgatgcgg cagatgcgat ggaaaaggtc gtcactggcg aagcggatct
    26761 ggcgattgcc gggaaaccgg agactctgcc cggcgcagtg gcattttcga tgctggagaa
    26821 tctggcagta gtgctgattg cccctgcgct accctgcccg gtgcgtaacc aagtttcggc
    26881 agataagccg gactggtcta cagtgccgtt tatcatggcc gatcagggac cggtgcgccg
    26941 ccgcattgaa ctgtggtttc gacgcaacaa aatcagcaac ccgatgattt acgccacggt
    27001 cggcgggcat gaagcaatgg tctcgatggt ggcgctcggc tgcggcgtgg cattgttgcc
    27061 ggaagtggtg ctggaaaaca gcccggaacc ggtgcgtaac cgcgtgatga ttttagagcg
    27121 cagtgatgaa aaaacgccgt ttgagctggg cgtgtgcgta caaaaaaagc ggcttcatga
    27181 gccgctgatt gaggcgttct ggaagatttt gccgaaccac taacccgcca aaaagaacct
    27241 gaacgccggg ttattggttt cgtcgtggca atcgtagccc agttcattca gccgggtttc
    27301 aaaatccggt tcatgatcgc caagctcgaa cgccgccagt acgcgcccgt agtcggtgcc
    27361 atggctgcga tagtggaaca aagaaatgtt ccagtgcgta cccagcgtgt tgaggaagcg
    27421 caacagcgcg cccggtgatt ccgggaattc gaagctgtag aggcgttcct gcaacggatg
    27481 cgatggacgc ccgccaacca tatagcgcac atgtagcttc gccatttcgt cgtcggagag
    27541 atcaaccacg ctgtagccgc cgtcgttgag catctgcaaa atttctttgc gctcttcgag
    27601 accgcggctc aggcgcacgc cgacaaaaat gcaggcgttt ttggcatcag caaagcggta
    27661 gttgaactcg gtgaccgagc gcccaccaag taactggcag aatttgagga agctgccctt
    27721 ttcttccgga atggtcaccg ccagtaacgc ttcacgctgt tcgcccagtt cgcagcgttc
    27781 cgagacgtag cgcagaccgt gaaagttcac gttggcaccg gaaagaatat gcgccagccg
    27841 ttcaccgcga atgttgtgtt gggcgatgta ttttttcatc cccgccagcg ccagcgcgcc
    27901 ggagggttcc gccaccgcgc gcacatcttc gaacaggtct ttcatcgccg cacatatggc
    27961 atcgctatcg acggtgatga tgtcgtcgag atactcctgg cataaacgga aggtttcatc
    28021 gccgatgcgt tttaccgcga cgccttcggc gaacagcccc acacgcggca gatcaacagg
    28081 atgaccggca tccagcgccg ctttcagaca ggcggagtct tccgcttcca cggcgatcac
    28141 tttgatttgc ggcatcagtt gtttgatcaa caccgccacg cccgccgcca ggccgccgcc
    28201 gccgaccggc acaaatacgc ggtcgagatg agcatcctgc tggagtagtt ccagcgccag
    28261 cgtcccttgc ccggcgatca ccatcggatg gtcgaacggc ggcacccagg tgaagccctg
    28321 ctgctgtgac agttcgatcg ctttcgcttt tgcttcatca aagttcgcgc cgtgaagaag
    28381 cacttcgccg ccgaagccgc gcaccgcgtc gactttgata tcggcggtgg cggttggcat
    28441 gacgatcagt gccttcacgc ctaaccgtgc ggaagaaaac gcgacgccct gcgcgtggtt
    28501 acccgcagaa gcggtgatca cgccgtgcgc tttctgttct tccgtcaggc ccgccatcat
    28561 ggcgtatgcg ccgcgcagct taaagctatg aactggctga cggtcttcgc gtttcaccag
    28621 aatcacgtta tcgagacgcg acgagagttt ttccattttt tgtagcggcg tgacctgcgc
    28681 cgcttcgtag accggcgcgc gcagcaccgc tcttaaatat tcggcacctt ccggggcacc
    28741 ggacaggggt tgtgagtctg ccatcattaa ccccccagtt tcgatttatc acgcaccgcg
    28801 cctttgtcgg cgctggttgc caggctggcg taggcacgca gcgcaaagga aacctgacgt
    28861 tcacgatttt tcggtgtcca ggctttgttg ccacgagctt cctgcgcttc acgacgcgcc
    28921 gccagttcag catcgcttac ctgtaactga atgccacggt tcggaatgtc gatagcgatc
    28981 aggtcaccat cttcaatcag gccaatgctg ccgccacttg ccgcttccgg tgagacgtgg
    29041 ccgatggaaa gaccagaggt gccgccagag aaacgaccgt cggtgatcag cgcacaggct
    29101 ttaccgagac ccattgattt caggaaactg gttgggtaga gcatttcctg catccccgga
    29161 ccgcctttcg ggccttcata gcgaattact accacatctc cggcgacaac tttaccgccg
    29221 agaatcgctt ctaccgcgtc gtcctggctt tcgtagactt tcgccgggcc agtgaatttg
    29281 aggatgctgt catcgacgcc tgcggtttta acgatgcagc cgttttctgc gaaattaccg
    29341 tagagcaccg ccaggccgcc gtctttgctg taggcgtgtt ccagcgagcg gatacagcca
    29401 ttggagcgat cgtcgtccag cgtatcccag cggcaatcct gcgagaatgc ctgtgtagta
    29461 cgaatgcccg ccgggcctgc gcggaacata tttttaaccg cgtcatcctg ggtcagcata
    29521 acgtcgtatt gttccagcgt ttgcggcaac gtcaggccaa gcacgttttt cacatcacgg
    29581 ttcagtaacc ccgcgcgatc cagttcgcca agaataccga taacaccacc agcacgatga
    29641 acatcttcca tatggtattt ttgggtgctc ggcgcaactt tacacagctg cggaaccttg
    29701 cgggaaagct tatcgatatc actcatggtg aagtcgattt ccgcttcctg cgccgccgcc
    29761 agcaggtgaa gtacggtgtt ggtagagccg cccatcgcga tatcgagcgt catggcgttt
    29821 tcaaacgccg ccttactggc gatattacgc ggcagtgcac tttcgtcatt ttgctcgtag
    29881 taacgtttgg tcaattcaac aatgcgctta ccagcattga ggaacagctg cttacggtcc
    29941 gcgtgggttg ccagcagcga gccgttgccc ggctgcgaca ggcccagtgc ttcggtcagg
    30001 caattcatag agttagcggt aaacatcccg gagcaagatc cgcaggttgg acaggcggaa
    30061 cgttcaacct gatcgctctg ggagtcagaa actttcgggt ctgcgccctg gatcatcgca
    30121 tcaaccagat cgagcttaat gatctgatcg gacagtttgg ttttcccggc ttccatcggg
    30181 ccgccggaaa caaagatcac cggaatattc aggcgcaggg aagccatcag catccccggg
    30241 gtgattttgt cgcagttaga gatgcagacc atggcgtcgg cgcagtgagc gttgaccata
    30301 tactcaacgg aatcagcgat cagttcgcga gatggcagcg aataaagcat ccccccgtgg
    30361 cccatggcaa tcccatcatc caccgcaatg gtgttgaact ctttggcaac gccgccagcc
    30421 gcttcaattt gttcggcgac cagtttaccg agatcgcgca gatggacgtg acccggtaca
    30481 aattgggtga acgagttcac aaccgcgata atcggcttac cgaaatcggc gtcggtcatt
    30541 ccggtggcgc gccacagcgc acgagcaccc gccatattac gaccatgagt ggtggtggcg
    30601 gaacggtact taggcatact ttatttactc ccagtgtctg tctcgtaaat gggacggtgc
    30661 gtgccgtccc attttttgta tttattgatt aacttgatct aaccagcccc atttatcttc
    30721 ggtttcgcca gtgaagaggc cgaagaaggc ttgctgaatg cgtttggtaa ccgggccaca
    30781 acggccttcg ccaacctgaa taccgtctac gctgcgcact ggcgtgattt ctgccgccgt
    30841 accggacata aacacttcat ccgccaggta cagggattcg cgcgacagca cctgctcacg
    30901 cacttcaatt cccagctctt ttgccagttt gatgatggca tcacgggtaa tacccggcag
    30961 cgcggatgag gtgaacggtg gggtgaacag cacgccgtct ttcacttcaa acaggttttc
    31021 gcctgcgcct tcagagatgt aaccattcac atccaacgcg ataccttcct gataaccgtg
    31081 gcggcgcgct tcgctaccca ccagcaggga agagaggtag ttaccaccgg cttttgccgc
    31141 cgtcgggatg gtgtttggtg ctgcgcggtt ccaggaggaa accatcgcat cgatcccctg
    31201 ctccagcgct tctgcgccca gatacgctcc ccacgggaaa gcggcgataa tcacgtcggt
    31261 tgagtatccc gctggcgggt ttacgcccat gccaacatca ccaacgaaga tcagcggacg
    31321 gatataggcg ctggtgagat tgtttttgcg gatcacgtca cgacaagctt ccatcagctc
    31381 atcaatgctc tgcgaaaccg ggaagcgata gattttggcg gagtcatgca gacgctgcat
    31441 atgctcacga tggcggaata caaccggtcc tttgtgcgag tcgtagcaac ggatgccttc
    31501 aaaaaccgag gtgccatagt gcagcgcgtg cgacatcaca tgcaccttcg cgtcttccca
    31561 gcgaaccatc tccccattga accaaatgta atcagctttc ttcgtggtca tttttatatt
    31621 ccttttgcgc tcaggcgcgg atttgttgtg atgtggttgt gctctggcag atagcaacgt
    31681 gtgcgacgtc caccagttta tttaactgac taaacagtaa gtcgaccgac cgtgggctgg
    31741 caacggtcaa ttcgatattt atattttgtg catcgctggc ggcggccata ttcattgagc
    31801 agacgtggaa accacgatga cgcaccacgc gtaaaacacg ttctaaggtt tccggattga
    31861 agcgagccga tacattgacc tgatgttgca tcatgataat ttctccaaca tttctgaatt
    31921 actggcacct ggcggcacca gcggccagac gttctcaagt tcgtcgattg agacatgaag
    31981 caggtatggc ccatcactgt tcagcatggt gtcgagtgcc gcttcaacct ggtctttacg
    32041 ggtgatgtgt tggccaggga tgccgaaggc gctggctaac atgaggaaat cggggttatc
    32101 ggtaagggtt gtttcgctgt atcgttcctg gaaaaacagt tgctgccatt gtcgaaccat
    32161 ccctaaccgt tggttatcga gtaagacgat tttcaacggt aactgcttgc gttttacggt
    32221 gcccagctct tgcacattca tcatgaaaga gccgtcaccg gagatacaga cgacggtatc
    32281 gttcggtcgc gcgacttgtg cgccaaccgc cgccggtaaa ccaaaaccca tggtgcctaa
    32341 gccgctggag gtgatgaaat tttccgggcg agtgtgggcg atgtgctgcg cagcccacat
    32401 ctggtgctgc cccacatctg tggtcacgac gcaatccgca ggttttcgat ccgacagttg
    32461 ttttaacaac aacggcgcgt agatagcgtc accgggatgg tcgtaacgcc aggtatgttc
    32521 atcacgcagc tgcgcgcagt attgctgcca gtcattgata tttaacggct gctgtaatgc
    32581 tggtaacaga gcatttaaat caccctgtaa tgccacatgt gcctgacgca gcttgttcat
    32641 ttctgccggg tcgatatcca tatggataac gctggcgtgt ggtgcgaagg tgttcagttt
    32701 gccagtcacc cggtcatcaa aacgtgcacc cacggcgatc agtagatcgc actcctgcac
    32761 tgcgaagttc gccgctttgg tgccgtgcat ccccagcatg cccagatagt acggataatc
    32821 tgcttctact gcgcccagcc ctttcaacgt acaggtggca ggcatttttg tggcagcgag
    32881 aaattcacgc aaagccgaaa ctgcctgcgc catgcccacg ccaccgccaa cgtacagcat
    32941 cggtttttgc gcttttgcca gcatctgacg cgcttgctcg acttcggcat gtgggaaagt
    33001 cacttcgttt tcaacagtgg tgaaccacgg ttccagatcg ccgctggcta actggatatc
    33061 ttttggaata tcgaccaaaa ccggaccagg acgaccagag ctggcgacgt caaacgcttc
    33121 ggccatgata cgcggtaact cttccagtga ttgcaccagg aaactgtgct tggtgcaggc
    33181 taacgacaac cccagaatat ccacctcctg aaatgcgtca gtgccgataa acggtgcgga
    33241 tacctgaccg gtgatagcaa caacggggat ggaatctaac agcgcgtccg caagcccggt
    33301 tatcaggttg gttgcacctg ggccagacgt ggcgatgcaa acgccagttt tgccggtagc
    33361 acgggcataa ccgatagccg ccattgctgc accttgctcg tgtcggcaca gcaagtgttc
    33421 cacgccaccg tcatacaatg catcgtaaac cggcataatt gcgccacccg gataaccgaa
    33481 aacggtgttt acaccctgtg cccgcaacgc atgtaccacc cactgtgcgc cattcatagt
    33541 tagttccccg tcctgaatct tgagaaacag aattttgtgc tgttattcat tgtctgctcc
    33601 tcagttatgt ttttaaggtc aaaaaaaacc cccggacctt tcggtgcggg ggtcttagtt
    33661 cgttaaggct tgatctctaa gcctttcctc gtccaagtgc agccccgcac ggtgggataa
    33721 taatcaccac cacgctaatc acgaccaggc taatcactcg tagaagggct gtcattttgt
    33781 cttttcttgc atcttgttcg aaggaatgcc taaagagtta ccatagattt tgcgaatagc
    33841 acaagatatt ttttggccga tttttatgat gtctttaata atttcaagag aaattgttgt
    33901 tttttaaaaa gaaaatagtg agtgaaaaaa attcaatttc agtatgaatg ttgttccctt
    33961 cctcgtagtt catcctttgc acgctgcatt tttgcgagca tcattccacc gccagaacaa
    34021 ctcaacttca cttgcacaaa taaacaggaa tcagccacta aaattcacga attagcgggc
    34081 tacagcagcg atatcttatg cacataatgg tgactcaagg agggcttatg tcactgtcaa
    34141 ttgttcatac ccgcgcagcc ctgggagtaa atgcgccccc aatcactgtt gaggtacata
    34201 tcagtaaagg tctaccaggc ttaacgatgg tgggcttacc agaaacaacg gtaaaagaag
    34261 ctcgcgatcg cgtgcgcagc gccattatca atagcggata tgaatatccg gcgaaaaaaa
    34321 tcaccatcaa ccttgctcca gccgatctgc caaaagaagg gggacgatat gatttgccta
    34381 tcgctattgc gttactggcg gcctcagaac agcttacagc caataagtta gatgaatatg
    34441 aattagtcgg agaactggcg cttacaggcg ctctgcgtgg cgttcccggc gcaatctcca
    34501 gtgcaactga agctattaag tcaggcagaa aaattatcgt cgcgaaagat aacgaagatg
    34561 aagtggggct aattaacggt gaaggatgcc tgatagccga tcatctgcag gctgtctgcg
    34621 cgtttctgga aggtaagcac gctctcgaac acccgaaacc aactaatgca gtatcccggg
    34681 cgctacaaca tgatctcagt gatgttgtcg gtcaggagca aggaaagcga ggactggaaa
    34741 ttaccgccgc tggcgggcac aaccttttac tgattggacc gccgggaaca ggtaaaacaa
    34801 tgctcgccag ccgtattaat ggccttttgc cagatttaag caatgaagag gcactggaga
    34861 gcgctgcgat attaagtctg gtaaatgctg aatcagtaca aaaacaatgg cggcagcgcc
    34921 cgttccgctc acctcatcac agtgcgtcgt taactgcgat ggtgggcggt ggtgcaattc
    34981 cagggccagg tgaaatttcg ctggcgcata acggcgtgct ttttcttgat gagctacctg
    35041 aatttgaacg gcgtacactg gatgccttgc gagagccgat tgaatccggg cagatccatc
    35101 tttcacgcac acgagcaaaa ataacctatc cagcccgttt ccagcttgtc gcggcgatga
    35161 atcccagccc taccggacat tatcagggaa accataaccg ctgcacacca gaacagacat
    35221 tacgttatct caaccggctc tcggggccct ttctcgaccg cttcgatctc tcactggaga
    35281 tcccattacc gccccccggc attttgagta aaacggtagt gccgggagaa aacagcacca
    35341 ccgttaaaca acgtgtaatg gccgccagag agcgccaatt taagcggcag aataaactga
    35401 acgcctggct ggataatccg gaaatacgcc aattctgcaa gcttgaaagc gaagatgcgc
    35461 agtggctgga agaaacgctg atccatctgg ggttatcgat tcgtgcctgg cagcggttat
    35521 tgaaagttgc acgaaccatt gctgatattg atcagtctga cattatcaca cgtcagcatt
    35581 tgcaggaggc agttagctat cgtgcgattg accgtttgct catccatctg caaaaactac
    35641 tgacataaaa aaagggcatt tcgccctttt tattaatcgt cagaatcggt gtagtcttca
    35701 gcaccttcaa cctgcggttt accaccggaa agggtgtgaa aacgttttgg acgcttgata
    35761 cgcgtcatat acttggacca cacgcgttct gcttctgtca ctggttcacg ttcgccacgg
    35821 catactgcta cgaagagttt ctcttcctcg gtaaccggct cgcgtttgcc aagatccaac
    35881 tcattgaagg cataaccatg acgctcaagc agttgtgcct ctttgatggt gaagtcacca
    35941 tgacgagaga atccacgtgg ataatgttta ttgtcgaaat atcgattagt cgtcgtaaag
    36001 ctttccgcca tcctgcacgc tcctaattct ttgaccgagc tagttatggc gcggagtatt
    36061 agttacgctt gacagagtgt aaaacaaaac atttaaatca taacgacaaa taattttgcg
    36121 gagagcactg tggatacgga attgttaaaa actttcctgg aagttagccg aacgcgtcac
    36181 tttggtcgag cggctgaatc gctctatctg acccaatcag ccgtgagctt tcgaatcaga
    36241 caactggaaa atcaactggg tgtgaatctt ttcacccgcc acagaaacaa tatccgttta
    36301 accgctgccg gagaaaagct tctgccttat gcagaaacgc tcatgagcac ctggcaggcc
    36361 gcccgcaagg aggtggcgca tacctcacga cataatgagt tttctatcgg tgccagcgcc
    36421 tcgctgtggg agtgtatgct taatcagtgg ctgggacgct tgtatcaaaa tcaggatgtc
    36481 catacaggct tacagttcga agcgcgaatt gcccaacggc agtctctggt aaaacagttg
    36541 catgaacgcc agcttgatct tcttattaca actgaagccc ccaaaatgga cgaattttgt
    36601 agtcagttgc tggggtattt cactttagcg ctttatacca gtgccccttc taaactaaag
    36661 ggagatctta actatctgcg actagagtgg gggccagatt ttcaacagca tgaggcaggt
    36721 ttgatcggtg ctgacgaagt tcccattctg acaaccagtt ctgctgaact ggcacagcaa
    36781 cagattgcga tgcttaatgg ttgcacctgg ctacccgtca gttgggcacg taaaaaaggc
    36841 ggcctgcata ccgttgtcga tagcacaaca ctttcacggc cgctttatgc catatggcta
    36901 caaaatagcg ataaaaatgc gttgattcgc gatctattga aaattaacgt gctggatgaa
    36961 gtgtattaat ctgaatggct ggcaaggatg ccggtagaag gagttacttc ggagagggtt
    37021 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggcag gggcggagag
    37081 actcgaactc ccaacacccg gttttggaga ccggtgctct accaattgaa ctacgcccct
    37141 aattagggtg gcggaacgga cgggactcga acccgcgacc ccctgcgtga caggcaggta
    37201 ttctaaccga ctgaactacc gctccaccga attcttttac aaccaccggt ttataaccgg
    37261 cttac
//
LOCUS       DAZFFE010000034        36129 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFFE010000034 DAZFFE010000000
VERSION     DAZFFE010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.034"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(20..805)
                     /gene="ygcW"
                     /locus_tag="ACHJ69_001946"
     CDS             complement(20..805)
                     /gene="ygcW"
                     /locus_tag="ACHJ69_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5014936.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            1124..2401
                     /gene="yqcE"
                     /locus_tag="ACHJ69_001947"
     CDS             1124..2401
                     /gene="yqcE"
                     /locus_tag="ACHJ69_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO5014937.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIIFYAPSGVIADKFSHRKMITSAMIITGLLGLIMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVVQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGYWQDTLPAAEAYRNMWL
                     MGMAALGMVIIFTFLLFQKIRTADSAPAMASSK"
     gene            2428..3906
                     /gene="ygcE"
                     /locus_tag="ACHJ69_001948"
     CDS             2428..3906
                     /gene="ygcE"
                     /locus_tag="ACHJ69_001948"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO5014938.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFASHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSEN
                     AAVMEKFNIPRQMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCHEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYTTAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
     gene            complement(4378..5049)
                     /gene="queE"
                     /locus_tag="ACHJ69_001949"
     CDS             complement(4378..5049)
                     /gene="queE"
                     /locus_tag="ACHJ69_001949"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO5014939.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKEDATRLCIDTCIARNWRLSMQTHK
                     YLNIA"
     gene            5222..5824
                     /locus_tag="ACHJ69_001950"
     CDS             5222..5824
                     /locus_tag="ACHJ69_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFO5014940.1"
                     /translation="MFRIFIAFIFIFNLSGCGYNDIQTYDEQVNASWSEVLNQYQRRT
                     DLIPNLVASIKGYSSHEQEVLEAVTLARSQANRASSDLQKTPGDEQKLQAWQQAQAQV
                     TRTLGQLTIISERYPQLKAQELYQNLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKF
                     PAVLTAKVMDYTPKKNYLPDDVTAVSKAPTIDFSQNANAH"
     gene            5838..6509
                     /locus_tag="ACHJ69_001951"
                     /pseudo
     CDS             5838..6509
                     /locus_tag="ACHJ69_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            6746..7900
                     /locus_tag="ACHJ69_001952"
     CDS             6746..7900
                     /locus_tag="ACHJ69_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO5014941.1"
                     /translation="MRIFLLLLMLCGFQVFAERLPSVTDLTGTLTTEEQSALNQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQRYGVYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEQMLPPEQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQLSLPCLAWTVLMIVAIIVLWRFTSRPGPGIWMISMITPTVWFFCFQ
                     DDVIIRRVSVVCFSLFFAAICWQRLAVVFNMCRVFPMMLAPKNKNAKVKKQKKTPQFR
                     ESGRNSLFVVMLIIIVGWCLVFASNKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGEKSNRKTGNRNAHAHSRSSSRNSFRGGGGSSGGGGSSDRW"
     gene            7906..8778
                     /locus_tag="ACHJ69_001953"
     CDS             7906..8778
                     /locus_tag="ACHJ69_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO5014942.1"
                     /translation="MRLIFILFTLWCLPGLAQQIAVPELRQQVTDITGTLSTSEQQSL
                     TQQLQDITHKTRAQVAVLVVPSTGDDSIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNRLTSQHQY
                     SVNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSSVCISAIYLLDL
                     FLFSDKTFSIAVFFFFFTFTTIMVFTCLCVLQKKASGRSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(8838..10136)
                     /gene="eno"
                     /locus_tag="ACHJ69_001954"
     CDS             complement(8838..10136)
                     /gene="eno"
                     /locus_tag="ACHJ69_001954"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO5014943.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(10224..11861)
                     /gene="pyrG"
                     /locus_tag="ACHJ69_001955"
     CDS             complement(10224..11861)
                     /gene="pyrG"
                     /locus_tag="ACHJ69_001955"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO5014944.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(12089..12880)
                     /gene="mazG"
                     /locus_tag="ACHJ69_001956"
     CDS             complement(12089..12880)
                     /gene="mazG"
                     /locus_tag="ACHJ69_001956"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO5014945.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(12952..13287)
                     /gene="mazF"
                     /locus_tag="ACHJ69_001957"
     CDS             complement(12952..13287)
                     /gene="mazF"
                     /locus_tag="ACHJ69_001957"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO5014946.1"
                     /translation="MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(13287..13535)
                     /gene="mazE"
                     /locus_tag="ACHJ69_001958"
     CDS             complement(13287..13535)
                     /gene="mazE"
                     /locus_tag="ACHJ69_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO5014947.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(13613..15847)
                     /gene="relA"
                     /locus_tag="ACHJ69_001959"
     CDS             complement(13613..15847)
                     /gene="relA"
                     /locus_tag="ACHJ69_001959"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO5014948.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(15895..17196)
                     /gene="rlmD"
                     /locus_tag="ACHJ69_001960"
     CDS             complement(15895..17196)
                     /gene="rlmD"
                     /locus_tag="ACHJ69_001960"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO5014949.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LECFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVAPEDCVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            17253..20009
                     /gene="barA"
                     /locus_tag="ACHJ69_001961"
     CDS             17253..20009
                     /gene="barA"
                     /locus_tag="ACHJ69_001961"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO5014950.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNICQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(20249..21589)
                     /gene="gudD"
                     /locus_tag="ACHJ69_001962"
     CDS             complement(20249..21589)
                     /gene="gudD"
                     /locus_tag="ACHJ69_001962"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO5014951.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGTYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPLEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(21610..22914)
                     /gene="gudX"
                     /locus_tag="ACHJ69_001963"
     CDS             complement(21610..22914)
                     /gene="gudX"
                     /locus_tag="ACHJ69_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO5014952.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVKAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTDLPYLENTPGNHE
                     WYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDTVRALKKRFPDAR
                     ITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAEFRRATGLPVATN
                     MIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLTWGCHSNNHFDIS
                     LAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVPDAPGLGVELDWE
                     QVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(22916..24268)
                     /gene="gudP"
                     /locus_tag="ACHJ69_001964"
     CDS             complement(22916..24268)
                     /gene="gudP"
                     /locus_tag="ACHJ69_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO5014953.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(24702..25151)
                     /gene="yqcA"
                     /locus_tag="ACHJ69_001965"
     CDS             complement(24702..25151)
                     /gene="yqcA"
                     /locus_tag="ACHJ69_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO5014954.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTMLS"
     gene            complement(25169..25951)
                     /gene="truC"
                     /locus_tag="ACHJ69_001966"
     CDS             complement(25169..25951)
                     /gene="truC"
                     /locus_tag="ACHJ69_001966"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO5014955.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGELLA
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(25951..26280)
                     /gene="yqcC"
                     /locus_tag="ACHJ69_001967"
     CDS             complement(25951..26280)
                     /gene="yqcC"
                     /locus_tag="ACHJ69_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO5014956.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPLPHQFASTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(26902..27447)
                     /gene="syd"
                     /locus_tag="ACHJ69_001968"
     CDS             complement(26902..27447)
                     /gene="syd"
                     /locus_tag="ACHJ69_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO5014957.1"
                     /translation="MDDLTARALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQSTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     IWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            27515..28363
                     /gene="queF"
                     /locus_tag="ACHJ69_001969"
     CDS             27515..28363
                     /gene="queF"
                     /locus_tag="ACHJ69_001969"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO5014958.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAIGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNNDFVPST
                     TRLVRQ"
     gene            28475..29839
                     /gene="ppnN"
                     /locus_tag="ACHJ69_001970"
     CDS             28475..29839
                     /gene="ppnN"
                     /locus_tag="ACHJ69_001970"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO5014959.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            30396..31685
                     /gene="sdaC"
                     /locus_tag="ACHJ69_001971"
     CDS             30396..31685
                     /gene="sdaC"
                     /locus_tag="ACHJ69_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO5014960.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPIIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            31743..33110
                     /gene="sdaB"
                     /locus_tag="ACHJ69_001972"
     CDS             31743..33110
                     /gene="sdaB"
                     /locus_tag="ACHJ69_001972"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO5014961.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLFLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            33222..33977
                     /gene="xni"
                     /locus_tag="ACHJ69_001973"
     CDS             33222..33977
                     /gene="xni"
                     /locus_tag="ACHJ69_001973"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO5014962.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHNEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(34082..35230)
                     /gene="fucO"
                     /locus_tag="ACHJ69_001974"
     CDS             complement(34082..35230)
                     /gene="fucO"
                     /locus_tag="ACHJ69_001974"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO5014963.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVIDKMDAAGLAWAIYDGVVPNPTITVVKEGLDVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(35258..35905)
                     /gene="fucA"
                     /locus_tag="ACHJ69_001975"
     CDS             complement(35258..35905)
                     /gene="fucA"
                     /locus_tag="ACHJ69_001975"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO5014964.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EANLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         8975 a         9354 c         8625 g         9175 t
ORIGIN      
        1 cagaaaccac cgttatggat tagcgcacta aataaccgcc atcaaccact aataaatgtc
       61 cgttgacata attcgatgcc ggacttgcga ggaatacggc tgcgcccatt aaatcctgag
      121 tatcgcccca acggtttgcc ggaatatgat caagaacgcg ctggttagtt tctggattac
      181 tacgggtcgc cagagtgata tcggtagcgt aatatcccgg ggcaatacca ttcacctgaa
      241 tattatattg acctaattca tcgcaatagg ctttggtgaa tccggcaaga gcatgtttag
      301 tggcagaata agccggtgac cattgaccac ctaagtaaga gaacaatgaa cagatattaa
      361 tgattttacc gcttttctgc gggatcataa tttttgcagc ttcatagctt aactcgaatg
      421 cggcggtaag gttcacgtca atcatcggat cccagtcggc acgcccgaag tccagcacct
      481 tattcagctt acatataccg gcattgttaa ccagaatatc gacggtaccg aaacgctcac
      541 agcaggcagc gataatcttc tgcggtgcgc cttctgcggt gatatccacc tgcatgaagt
      601 ccacttcaac accctgtttt tcaatcattt cctttgtttc gccgttatct ttgacgaaac
      661 ttggaataaa gacatttgcg ccagccttgg ccagcgccat ggcaaatgcc tggcctaatc
      721 cgctattccc gccggtaaca attgctgttt tacctttcag ggagaaaaaa tccattgaga
      781 acgcattgag agattcgatt gacatagtta gctccaattt ccgtaaggca aaaaaaaaga
      841 aaggtaactt cccccgcgag agggaagtta cctttctcat tatctctggt aactggagat
      901 aatttttagc ataagggcat aactgctaat gtgatcgtaa tcacagagtg atgttcgtat
      961 tatcaaatag ttttaaaaag cagaagcgtg attactctca cacaacacct tttctttcca
     1021 tgttatttct tattgcaatt actagagacc tgagaaaagt aatccacctg aaagggcggc
     1081 ttacttttct cttttttttg tctattacaa atgggaatat gatatgcaac acaactcata
     1141 tcgccgttgg ataaccctcg cgataattag ttttagcggc ggcgttagtt tcgacctggc
     1201 ttatttacgt tatatttatc aaattcccat ggcaaaattt atgggattca gcaataccga
     1261 gataggttta ataatgagta cctttggtat tgcggccatt attttctatg cccctagcgg
     1321 cgttattgcc gataaatttt cacaccgcaa aatgattact tccgcgatga tcattaccgg
     1381 attactgggc ctgataatgg caacatatcc accactgtgg gtaatgctct gtattcaggt
     1441 cgcctttgcg ataacgacga ttttaatgct gtggtcggtg tcgattaaag ccgcatcgtt
     1501 gctgggcgat catagcgagc aagggaaaat tatgggctgg atggaagggc tgcgcggcgt
     1561 cggtgtaatg tcgctggcgg tgtttaccat gtgggtcttt tctcgctttg caccggatga
     1621 cagcgccagc ctgaaaacgg tcattattat ctatagtgtg gtttacattc tgctgggcat
     1681 tttgtgttgg ttttttgtta gcgataacaa caatctgcgc agtgccaata acgaagaaaa
     1741 acagtcattc cagcttagcg acatcctggc cgttttgcgc atcagcacca cctggtattg
     1801 cagcatggtg atttttggtg tcttcaccat ctacgccatt ctgagttact ccaccaacta
     1861 tctgaccgaa atgtacggca tgtcactggt ggcagcgagc tacatgggga ttgtgatcaa
     1921 caaaatcttc cgcgcgttat gcggcccact tggcggcatt atcaccacct acagcaaagt
     1981 gaaatcccct acccgcgtgg tccaaatcct ttccatcatc ggtttgctgg cattgacagc
     2041 cctgctcgtc acgaactcta acccgcaatc ggtcgcgatg gggattggct tgattttatt
     2101 actgggattc acctgttacg cctcacgcgg gctgtattgg gcctgccctg gcgaagcgag
     2161 aacaccgtct tacattatgg gcaccacggt aggtatttgt tcggtgattg gattcctgcc
     2221 ggatgtcttc gtttacccga ttatcggcta ctggcaagac accctgcccg ctgcagaagc
     2281 ctaccgcaac atgtggctaa tgggcatggc ggcgcttggc atggtgatca tctttacctt
     2341 tttgctgttc caaaaaattc gtactgctga tagcgccccc gcaatggcta gcagcaagta
     2401 agccaggaaa attacaggag aaaaatcatg tcgaaaaaat acatcatagg gattgatggc
     2461 ggaagtcaga gcacaaaagt ggtgatgtac gatctggaag gtaatgtggt ttgcgaaggc
     2521 aaaggcttat tgcagccgat gcacacgcca gatgccgata ccgcagaaca tcctgacgac
     2581 gatttatggg catcactatg ttttgccagt cacgatttga tgagccagtt tgccggaaat
     2641 aaagaagata ttgtcggcat tggcctgggc tcaatccgtt gctgccgtgc gttattgaaa
     2701 gccgatggta cgccagccgc gccgttgatt agctggcagg atgcacgcgt tacacgccct
     2761 tacgaacaca ctaatcctga cgtggcctat gtcacctctt tttcgggtta tctgacacat
     2821 cgcttaaccg gcgagtttaa agacaatatc gccaactatt ttggtcagtg gccggtggat
     2881 tataagagct gggcatggag cgaaaatgcc gcggtaatgg aaaagtttaa tatcccccgc
     2941 cagatgcttt ttgacgtgca aatgccgggc actgttcttg ggcatatcac accacaagct
     3001 gcgctggcga cacattttcc ggctgggctg ccggttgttt gtaccaccag tgataaaccg
     3061 gtagaagctc tgggagccgg gttactggat gatgaaacgg cggtaatttc tcttggcacc
     3121 tatatcgcgt tgatgatgaa cggcaaagca ctgccgaaag atccggtcgc gtactggccg
     3181 attatgtctt ctattccgca aacattgctg tatgaaggtt acggtattcg caaaggcatg
     3241 tggacggtga gctggctgcg cgacatgtta ggcgagtcgt taattcagga tgccaaagca
     3301 caggatcttt caccggaaga tttgctcaac aaaaaagcgt cttgtgtgcc gcctggctgt
     3361 aatggtctga tgacggtgct ggactggctg accaatccgt gggaaccgta caaacgcggg
     3421 attatgatcg gttttgattc cagcatggat tacgcatgga tatatcgttc gatactggaa
     3481 agcgtggcgc tgacgctgaa gaataattac gacaatatgt gtcatgaaat gaatcacttt
     3541 gcaaagcatg tgatcattac tggcggcggt tcgaacagcg atctgtttat gcagattttt
     3601 gccgacgtgt tcaaccttcc ggcacgccga aacgccatta acggttgtgc aagtctgggg
     3661 gctgcgatca atacggcggt aggtctgggg ctatacccgg attacacaac ggctgtcgat
     3721 aaaatggttc gcgtgaaaga tatcttcata ccgattgaga gcaatgccaa acgctacgac
     3781 gcgatgaata aaggcatttt caaagaccta accaaacata ctgatgtgat cctgaaaaaa
     3841 tcgtatgaag tgatgcatgg ggaattgggg aatgcggatt cgatccagag ctggtcgaat
     3901 gcgtaagtga ggaaggccgg gcgggaaact gtccggcctg aacatacctg aatggttatc
     3961 cccgctgacg cggggaactc atatccggtt tttgctggtg ggccgcgccg ttcggtttat
     4021 ccccgctggt gcggggaact ctaaacataa cctattatta attaattatt ttttaagaca
     4081 gtcacaatct accaacttta cagtatcaca caaacaacac atccattatg ttaaagagca
     4141 cttaatccat tgattaaaaa gataaatatt tacaatgact ctattcaaac taaaatctac
     4201 caaactttac cgcaataatt ttcactccag cgaaaaatta acgccacaga atttgtagag
     4261 aatgataatg attagtccag aatgggcaaa atggaggttt ttcagagacg acaaagaata
     4321 taaatgagta gaagtcttta cagattccac ccatttataa cgcttataaa tgtttaatca
     4381 ggcaatattc aaatatttat gtgtctgcat cgacaaacgc cagttgcgcg caatgcaggt
     4441 atcgatacat aaacgcgtag catcttcctt ctggctaatt ggctgaagtg caatgactcg
     4501 cggtttatca tcggtcagcg tcgccagcaa ttcatccagt gcttcaatat cacgtacgcg
     4561 tcctactggg tgtttgattt cgttggctcg ctccagggct tgtgacaaca cttcatagcc
     4621 accgcgcata tttagctttg gtgatacggt aacccaggta ttcggtgtgc agcgtacttc
     4681 atgagtacca ctagtttcga tctggcagct aaaaccgttc ttttcgagca ggtcagtcag
     4741 tggcagcaaa tcatgaatgc aaggctcacc acccgtaatc accacatgac gcgcggtgta
     4801 tccctgacgg ccaatgacag ccagcaaatc ttcactgctc gccgcccccc acttatcact
     4861 ctctttggtc ttcgccagaa tgctgaaaag ggagacttcc cgatcctcaa gcttttccca
     4921 ggtgtgtttg gtgtcgcacc aggcacagcc aaccggacat ccctgtaaac gaataaaaat
     4981 ggcggggacg ccggtaaagt aaccctcacc ttgcagggtc tggaacatct cgttaatcgg
     5041 gtactgcata gcattctctg tgaagtggat aattgttaat tattgcagat cctgccacaa
     5101 caatcatgtc ttattaacat tctgttacag gcaggtttga tgcaaaaatt ggctcagatc
     5161 attaagaata aaatgaagcg gcgtattatg ttttcaagga ttaaatgaaa gggatttaaa
     5221 catgtttaga atattcatcg cgtttatatt tattttcaat ttatcaggtt gcggttataa
     5281 cgatattcaa acctatgatg aacaggttaa tgcctcctgg tcagaagtat taaatcaata
     5341 tcaacgtcgc accgatctta ttcctaatct tgtcgctagt attaaaggtt actccagcca
     5401 tgaacaagag gtactagaag ccgtaacatt ggcacgtagt caggctaatc gcgccagtag
     5461 cgatcttcag aaaactccgg gagatgaaca aaaattacaa gcctggcaac aggcgcaggc
     5521 gcaggtgact cgcactctcg ggcagttaac gattattagt gagcgttacc ctcagttaaa
     5581 ggctcaagag ctctatcaaa acttaatggt acagctcgaa ggaagtgaaa atagaattgc
     5641 cgttgcgcga ggaagatata ttaaagccat tgaacaatat aatgtcacta tccgcaaatt
     5701 ccccgctgta ttaaccgcaa aggtcatgga ttacacacca aagaaaaatt atttgccgga
     5761 tgatgtcacc gcagtaagta aagccccaac aatagatttt agccagaatg ctaatgctca
     5821 ttaagcgggg acgttctatg cgaaatttta tcttccttat ggctttcttc tgttcatctg
     5881 tgtttgctac acaaattcca gtacctgaat cacccaggta tgtgaatgac ttaaccggca
     5941 cattaacaaa cagcgaagta aacaccctga ccaaccagat taaagccctg acgcaaaaaa
     6001 accacgcaca gttggtcgtg ttagtggttg atacaacagg cgatgaaacc atcgagcagt
     6061 acgcaacgcg ggtattcgat agctggaaac cgggtgataa agaccgtgat gatggcgttc
     6121 tactgttagt cgcctggcag gatcataccg tgcgtattga aatcggttac gggctggaag
     6181 gtattattac ggatgcacag tccgggaaaa ttattcgcaa cagtattatt ccggcattta
     6241 aaaaaggcga cctggctggt ggtttacaaa aggggatcaa cgatattgaa agtcgcctga
     6301 cggggaataa ttcagcaacg ataacaccga ccgatcaccc tttgcccttt agcggttggt
     6361 gggctttatt agtctgggcg ataatactga cgtttatttc tgccagagga tatatcaaaa
     6421 cgttgggagt aatttgcttc gcagccattg tactggcctt tgttttacct atagcaggtt
     6481 tcagcggaag ctggggagta ctcacaacat tactctgctt cgccacaccg tttctggcag
     6541 ttgccatcgc ttttaccccc ttcggcaaaa aggttcggga ttcgatgcgt gatgccaacc
     6601 agccatcacg ccatacaaga agcaattctt cctcccacta ttcatcatcg tcatcctctt
     6661 cattcgacaa tgacaatttt agcggtggtg gcggttcatc tggtggcggt ggcgcttccg
     6721 ggcgctggta acaaaaggaa ttgatatgcg tatttttctc ttacttctga tgctatgcgg
     6781 ttttcaggta ttcgctgaac gattgccttc agtgactgac ttaaccggga cattaacgac
     6841 agaagaacaa agtgctttaa atcaacaatt acagactctc gagcagcaaa accacttcca
     6901 ggtggccgtg ttggttacgc cgacaacagg gggcaaaaac attaaacttg cagcctcgca
     6961 gagatacggg gtatatcact ggaaacccgt tggagagaag cgatatggcg aaggtatctt
     7021 aattctcgtc gtttggccgg aagggctggc gtcaatgaag attggacatg gactggaaca
     7081 gatgttgccg ccggaacaag ctgcgcaaat tgttcgttac catatgcagc cagaattcga
     7141 aaaaaacaat ctctttgctg gattaacggg cggaattgaa agcatcgcac agtttaccca
     7201 tatcaaagcg aaccttagcc ctttggatgc tttagccaat catctgtttg ctaatccgca
     7261 actttcgctg ccatgccttg cctggacagt gctgatgatc gttgccatca ttgtcctctg
     7321 gcgatttacc agccgtccag gacctggcat atggatgata tcaatgatta cacctacggt
     7381 ctggtttttc tgctttcagg acgatgtcat cattcggcgg gtgtcggtcg tttgttttag
     7441 tttatttttc gcagcaatct gctggcagcg tctcgcagta gtttttaata tgtgccgcgt
     7501 atttcctatg atgctggccc caaaaaataa aaacgctaaa gtgaaaaaac aaaagaagac
     7561 tccgcaattt cgtgaatcag gacgaaattc attgtttgtc gtgatgctga taattattgt
     7621 gggatggtgc ctggtgttcg ccagtaataa agatattgca ttcatctcaa caattattgg
     7681 gttaattgat cattttatcg gcccaataac cattgctggc atcgttctgc tgatcattgt
     7741 ggccaaattt actggcaatc ttaagctggg gtcaggagaa aaaagcaaca ggaaaacggg
     7801 gaatcgtaat gctcatgctc attctcgctc ttcgtcgaga aattctttcc gcggtggtgg
     7861 cggttcttca ggaggcggcg gttcatccga ccgttggtga tcgtaatgcg cttaatcttt
     7921 attctattca ctttgtggtg ccttcctggg cttgcccagc aaattgctgt gccggaactg
     7981 cgccaacagg tgaccgatat tactggtacg ttaagcactt ctgaacaaca atcactgaca
     8041 cagcaactgc aggatattac acataaaaca cgggcgcagg tcgctgtatt agtagtgccg
     8101 tccaccggag acgattccat tgaacaatat gctacaaggg tttttgacaa ttggcgttta
     8161 ggagatgcca aacgtaatga tgggatactg atcattgttg cctggtcgga tcgcactgtc
     8221 cgcatccagg taggttatgg gctggaagaa aaggtaaccg acgctctggc tggagatatc
     8281 atccgtagca acatgatacc cgcatttaaa caacaaaaat tagctaaggg attagagtta
     8341 gctataatcg ctttgaataa ccgactcact tcacaacacc aatattcggt taatccttca
     8401 gaaagtgaat cagcatcttc cagcgatcat tactactttg ctattttttg ggtatttgca
     8461 gtgatgttct tccctttctg gttttttcat caaggcagta atttttgtcg cgcatgtaaa
     8521 agcagtgtct gtatttcagc aatctatctt ttagatttat tcctgttctc cgataaaact
     8581 ttttccattg ctgtattttt cttctttttt actttcacca ccattatggt ctttacctgt
     8641 ttatgtgtac ttcagaaaaa agcatctggt agaagttatc attcagacaa cagcggttcc
     8701 ggtggtggct caagtggcgg cggatttagc ggcgggggcg gttcttctgg cggtggcggc
     8761 gcgtccggtc gctggtaact caaccataaa aaatgccagc ccgaaggctg gcatttttaa
     8821 atcagataaa gtcagactta tgcctggcct ttgatctctt tacgaccgtt gtacggtgct
     8881 ttttcgccca gagcttcttc gatacgaatc agctggttgt atttagcaac acggtcagaa
     8941 cggctcatag aaccagtttt gatctggcct gcagcagtac caacagccag gtcagcgatg
     9001 gtagcgtctt cagtttcgcc agaacggtga gagataactg cagtgtagcc agcgtctttc
     9061 gccatcttga ttgcagccag agtttcagtc agagaaccga tctggttgaa tttgatcagg
     9121 atggagttag cgataccttt ttcgatacct tctttcagga tcttggtgtt ggttacgaac
     9181 aggtcgtcac caaccagctg gattttgtcg cccagaactt tggtctggta tgcgaaaccg
     9241 tcccagtcag attcgtccag accgtcttcg atggaaacga tcggatactg tttggtcaat
     9301 tcttccagga agtgagtgaa ttcttcagag gtgaacgctt tgttgccttc gccagccaga
     9361 acgtatttac cgtctttgta gaattcagaa gctgcgcagt ccatcgccag agtgatgtct
     9421 ttgcccagtt cataaccagc agctttaaca gcttcagcga taacagccag agcttcagcg
     9481 ttggaaccca ggttcggcgc atagccacct tcgtcaccaa cagcagtgtt catgcctttc
     9541 gctttcagaa cttttgccag gtgatggaaa acttcagaac ccatgcggat ggcttctttc
     9601 acagttttcg cgccaaccgg ctgaatcatg aattcctgga tatcaacgtt gttgtcagcg
     9661 tgctcaccac cgttgatgat gttcatcatc ggaaccggca tagagtattt gcccggagtg
     9721 ccgttcagtt cagcgatgtg ctcgtacagc ggcatacctt tagcagctgc agcagctttg
     9781 gcgttagcca gagatacagc caggattgcg ttcgcgccga atttggattt gttttcggtg
     9841 ccgtccaggt cgatcatgat cttgtcaatg ccagcctgat ctttagcatc tttgccaatc
     9901 agcgcctgag cgatcgggcc gtttaccgca gcaacagctt tggttacgcc tttacccagg
     9961 aaacgggatt tgtcgccatc gcgcagttcc agagcttcac gggaaccagt agaagcacct
    10021 gacggagcag ctgccatacc gacgaaacca ccctccagat gtacttcggc ttcaacagtc
    10081 gggttaccac gggagtcgat gatttcacga ccgatgattt ttacgatttt ggacattagg
    10141 ttttcctcaa gtcactagtt aaactgaaac tccagacaaa caacgcgtac ccagggtacg
    10201 cgttgccgtt ctaacttttt tacttacttc gcctgacgtt tctggaactc gctggcggct
    10261 ttcacaaagc ctgcaaacag cgggtgacca tcacgtggag tagaagtaaa ctccggatgg
    10321 aactggcaag ccacgaacca cggatgattc ggaacttcga tgatctcgac caactgatca
    10381 tccccggaac ggcccgcaac gcgcagacct gcatcttcaa tctgtttcaa cagcatgttg
    10441 ttgacttcgt aacggtgacg atgacgctca acaattgtcg gcgcattgta cagctggcga
    10501 accaggctat cgtcaaccaa ctggcactgc tgtgcgccga gacgcatggt accgccgaga
    10561 tcgctcttct cgctacgaac ttcaacgttg ccgttttcat cgcgccactc ggtaatcagc
    10621 gccacaaccg ggtacttaca gtctggcaca aattccgtag agttggcgtt ctccatgttg
    10681 gcaacatggc gagcgtaatc aattaacgcc acctgcatac ccaggcaaat gcccagataa
    10741 ggaatattgt tctcacgcgc aaaacgcgcg gtcgtaatca tgccttctac gccacgatag
    10801 ccgaaaccgc caggtacgag gattgcgtcc agacctttga ggatttcaac gccgcgcgtt
    10861 tcaacatctt gtgaatcgat cagtttgatg ttgacgctga cacgattctt cagcccaccg
    10921 tgtttcagtg cttcgatcac ggatttgtat gcatccggca gctcaatgta tttgccgacc
    10981 ataccaatgg tgacttcact taccggattc gcttcttcga agataacctg ttcccattcg
    11041 gacagattcg cttccgggca gtttaagctg aatcgtttac aaatataatc gtccagcccc
    11101 tgagatttca acaggcccgg aattttatag atggaatcga cgtctttcag agaaataacc
    11161 gctttttccg gaacattaca gaacaatgca atcttcgcac gttcgttcgc cggaacggcg
    11221 cgatctgaac gacaaatcag gatatcaggc tggataccga ttgaaagcag ctcttttaca
    11281 gagtgctgag tcggtttggt tttgacttca cctgacgctg ccatgtacgg caccagcgtc
    11341 aggtgcataa acagggtgtg ctcacggcca atttcaacag ccatctggcg aatcgcttcg
    11401 aggaacggca gggattcgat atcaccgaca gtaccgccga tttcgaccag tactacgtca
    11461 tgaccttcgc cgccttccag cacgcgctct ttgatagcgt tagtgatgtg cggaataacc
    11521 tgtacggttg cgccgagata gtcaccgcgg cgttctttac gcagaacgtc agagtagata
    11581 cgacccgtgg tgaagttgtt gcggcggctc attttggtac gaatgaaacg ctcgtagtgc
    11641 cccaggtcca ggtcggtttc agcgccgtct tcagtaacga acacttcccc gtgttggatt
    11701 gggctcatag tacctggatc gacgttgatg tacggatcca gtttcatgat ggtcacattg
    11761 aggccacggg cttcaagaat ggctgcgagg gaggctgcgg caatgccttt acccagagag
    11821 gatacgaccc cgccggtcac aaaaatatag ttcgttgtca tgctgaacct gagaagttag
    11881 gttgaaaaga cgatggaata accaggacgg gaaagcagta tacccgaaca tgacctgtgc
    11941 cacaaacttt cattatccct cctcttcgcc agcgcactat tgaaatcagg agtgagaaaa
    12001 tagccccttt ggggtaaatg tttttgacgc aaatcaagcg cttgtcattt aaaaaatgac
    12061 acaaatcgcg cttgaccgcg taattccctt agagatcaat ttcctgccgt tttacctgtt
    12121 gccagacttc ttccattgtt tcgaggtcaa cacctgtcat ttccagtcca cgcgcggcaa
    12181 caatacgctc cacttcgcga aaacgacgct cgaatttttc gttcgctttt tgcaatgcga
    12241 tttccgcttt tgtccctaaa tgacgagcca gattgaccgt ggcaaacagc aggtcaccca
    12301 tttcctcctc cagtttagcc tggtcgacaa cagcctgccg cgcctcgtac atcacctcgt
    12361 cgatctcttc gtagacttta tcgactaccg gaccaagcgt cgtccagtcg aaaccaacgt
    12421 tggcgcaacg tttctggatt ttttgcgcac gcattaaagc cggtaaacta cgaggaatat
    12481 cgtccagcgc cgaatgctgc gctttctgcg cgcgttcttc ggttttgatt tgctcccaac
    12541 gggcaagcac ttcactacta ttttcggcag aactgtcagc aaaaacatgc ggatggcgac
    12601 gctctaattt atcgctaata gcagcgcaaa tatcattaaa gtcaaagcgc ccttcttcct
    12661 gagccatttg tgcgtaaaac accacctgga acagcagatc gcccagttca ccgcgcagat
    12721 cgtcaaaatc ttcacgggcg atggcgtcca gcacctcgta ggtttcttca agggtgtaag
    12781 gcgcaatggt ggcaaatgtc tgctctttat cccacgggca gccgttttcc gggtcgcgca
    12841 ggcgctgcat gatggtgagc aaacggtcga tttgattcat tgaattatcc tgaaaattgc
    12901 gggtctgtca ggtggaaacc tgtgaccaga atagaagtga gttagtaaca cctacccaat
    12961 cagcacgtta attttggctt taatgagttg taattcctct ggggcaactg ttcctttctt
    13021 cgttgctcct cttgcccgcc aggcgatact ttttacctga tcagctaacg ctacgccatc
    13081 acgttcctga ccggatagaa caacttcgaa cggatatcct tttgattgcg ttgtacaagg
    13141 aacacacaga cacatacctg ttttgttgtt gtacatgaac ggactcagga caacagccgg
    13201 acgatgtccg gcttgctcgc taccttttgt cgggtcaaaa tcaacccaaa tcagatcgcc
    13261 cgtatcgggt acgtatcggc ttaccattac cagacttcct tatctttcgg ctccccccag
    13321 tcgatattct cgtggaggtt ttccggcgtg atgtcgttga ccagttcagc aagcgtaaat
    13381 acgggctctt tacgcactgg ctcaataatt aatttgccat ccaccaggtc aatcttcact
    13441 tcatcatcaa tattcagatt gagcgcctgc attaacgtag ccgggatccg caccgccggt
    13501 gaatttcccc aacgctttac gctactgtgg atcataaccc tttcctcaaa ccgcgatcat
    13561 atgtagatac agtatatatc aatctacatt gtagatacga gcaagtttcg gcctaactcc
    13621 cgtgcaaccg acgcgcgtcg ataacatccg gtacctggtt gagtttaccc agcacgcgac
    13681 ccagcacttg caggttgtaa atctcaatgg tcatgtcgat ggtcgccagt tgctgtttgg
    13741 tgtcgctacg gctggcaacg ccaagtacgt tcaccttctc gttggcgaga atggtcgtga
    13801 tatcacgtaa caacccacta cgatcattag ccaccacgcg gaccaccagc gaatatccgg
    13861 cggagtagct ctcgccccat accgcgtcaa caatgcgttc tggcgcatgg gagcgcagtt
    13921 ccgccaattg ttcgcaatcg gcgcggtgta ctgaaatacc gcgcccctgg gtaatgaagc
    13981 cgacaatctc atctccagga atcggctggc agcagcgcgc gatgtggtgc atcaggttac
    14041 cgaccccttc taccaccaca cgaccgttat ctttactgcg gttttgcggc gtgtagcttt
    14101 tttgctgaag ctgtttcagc gcggcggcgt cctgctcttc ggcactcggc ttattaaatt
    14161 gcgattgcag gaagttcacc atctgattga gacggatatc cccgccacca atcgccgcca
    14221 gcaactcgtc gacatcattg aagttgtaac gcggcagcag atgtttttct gcttctttca
    14281 ggctgatccc cagatgttcc agctcgtcgt caaggatttg ccgcccagcc agaatgtttt
    14341 tgtcacggtc ctgtttacgg aaccaggcgt gaattttcga acgcccacgg ctggttgtga
    14401 cgtaaccgag gtttgggttt aaccagtcac ggctggggtt cggctgtttc tgggtgataa
    14461 tttcaatctg gtcgcccatc tgcagctggt aggtgaacgg cacaatgcgc ccgccaattt
    14521 ttgccccgat gcagcggtgt ccgacatcac tgtggatgtg gtaagcgaag tccagcggcg
    14581 ttgatcccgc aggcaaatca acgacatcac ctttcggcgt aaagacgtac actcggtcgt
    14641 caaagacctg actacgtact tcgtcgagca tttcgccgga atcagccatc tcttcctgcc
    14701 acgcaatcag tttacgcagc caggcaatcc ggtcttcatg tcccgaacgt gcgccgccag
    14761 cagccgcgcc ctctttatat ttccagtgcg cagcaacacc caactctgca tcttcatgca
    14821 tctgtttggt gcggatttgg atctcaacgg ttttgccacc cggccccaga accacggtat
    14881 gaatggactg ataaccgttt ggtttcgggt tagcgacgta atcgtcaaac tcatccggca
    14941 ggtggcgata gtgagtgtgc actatcccca gtgcggcata acaatcctgt aaacgctcgg
    15001 cgacaatacg taccgcacgc acatcaaaca gctcatcgaa ggcgaggttc tttttctgca
    15061 ttttgcgcca gatactgtag atgtgtttcg gacggccata cacttcagct ttaacgcctt
    15121 cagctttcat ctcagcgcgc agatggccga caaactcttc gatatagtgt tcgcggtcga
    15181 gacggcgttc atgcagcagt tttgcgatgc gtttgtattc ggtcgggtgg agataacgga
    15241 agcagtaatc ttccagctcc catttcagtt gcccaatccc aagacggttc gccaacggcg
    15301 cgtagatatt ggtgcactct tttgccgcca gtacgcgttc atcttccggc gcatctttta
    15361 cttcgcgcag gtgagcaata cgctccgcca gtttgatgac cacgcagcga aaatcatcga
    15421 ccatcgccaa taacatccgg cgaacgttat cgacctgttc ggaggaaaca gaatcagtgt
    15481 gcgtcgcttt cagctggcgg atcgccgcca tatcacgcac gccgtgaata aggttaacga
    15541 ccgacttacc gacgctctca cgcagcacat cttcgctgac tacgttggca tcagccagag
    15601 ggaacagcag cgccgcccgc agcgtgtcaa tgtccatact taatgtcgag aggatctcca
    15661 ccatctcaac accacgccac aataacagac tggcatccgg atgcccctgc gtctgttgca
    15721 gacaatacgc ccaggtttcg gctaagcact cacacgactt ctggctggta atacccagac
    15781 ttgcgatcca tttttccgga tcaaattcac cagccttatt gatatgtgca cttcttaccg
    15841 caaccatcgt cctctccttt agggaccaga cctgccgaaa tcggcaaatc gtaactattt
    15901 aacgcgcgag aaaagtacca tcgattccag atgtcccgtg tgtgggaaca tatccagcat
    15961 cgccagtcgc gcaatggtat atcctgcttt taataacgct tcgctatccc gagccagcgt
    16021 tgcagggtta caggatacat aaactatacg aataggttcc agttttataa tttgctgcat
    16081 aacacctgcg gcacctgctc gcgccgggtc caacaacact ttatcgaagc cgtttttcgc
    16141 ccacggctgc tttgtgacat cttcttcaag attttcgtga taaaacgtca cattctgtaa
    16201 gccattaaga cgcgcattct gctggccttt ttccaccagc gccggaacac cttctacacc
    16261 caccacactg gcagcttgtg tcgccaatgg cagtgtaaag ttgcccatac cgcagaacag
    16321 atccagtacg caatcttcag gtgccacatc cagccattcc aacgcacgcg ctaccatttt
    16381 ctggttcaca cccgcattga cctgaataaa atcgcgcggg ctaaaagtta agcgcaaccc
    16441 atttgagtca taccagggca tctcaccaga gacggtttcg agtatctcac tatcgggggc
    16501 gagatacaga tccaggcctt cagaatgcga aaagcattcc agtttttcgc gatctgccga
    16561 acttagcggt gcggtatggc gcaaaatcat tagcgtgccg ctggttgcct gtaccagttc
    16621 aacatgacca agatggcgca tagcttgtaa gctgcccagg catgccctga ctttgggcag
    16681 caatgcttca agttggggca ctaaaatggg gcattgtttc acgtcgacaa tgtcactgga
    16741 gcccgctttg cgaaacccca tctgaagttg ctgtgttttc ggtaagtagt tcagacttaa
    16801 acgcgcgcga cggcgatagc cccagggaac atcggcgatg acttcagaga cttcgtgttt
    16861 cattaatcgg gcgagtgccg cacttttgct tcgctgctgt aaatccacac tggcgtgttg
    16921 ttgctgacag ccgccgcata cgccaaaatg aggacagcgt ggcgtttcgc gttccgggct
    16981 atcgcttaac cgacgtacga ctttagcgcg ggcatactgt tttttatctt cagtaacagt
    17041 aacttccgcg ttttcctgcg gcaataatcc ggggataaac aacgttttgc cgttatgtcg
    17101 cgccacgccc tgaccaaaag agtcgaggtc gttgactgaa acggttatga tctgacgcgt
    17161 cgtcgtgcgt cgttttgcag agtagaattg cgccattggc gagactttct caatttaaca
    17221 gtgtgacctt aattgtccca taacggaact ccatgaccaa ctacagcctg cgcgcacgca
    17281 tgatgattct gatcctggca ccgaccgtcc ttattggttt attgctgagt atctttttcg
    17341 tcgtgcatcg ctataacgac ttgcagcgtc aactggaaga tgccggtgcc agcattattg
    17401 agccgcttgc agtttctact gaatatggca tgagtctgca aaatcgcgaa tctatcggtc
    17461 agttaataag cgtactgcat cgtcgccatt ctgatattgt tcgcgcgatt tcggtttatg
    17521 atgaaaataa ccgactcttt gtcacctcca attttcatct tgatccctca tcaatgcagc
    17581 tcggcagcaa cgtgccgttt cctcgccagc tcactgtcac tcgtgacggc gatataatga
    17641 tcctccgcac gccgattatt tctgaaagtt actcccccga cgaatcgccc agtagcgatg
    17701 ccaaaaatag tcaaaatatg ctgggatata tcgcgctgga gctggatctt aaatcggttc
    17761 gcttgcagca atataaagag atctttattt ccagcgtgat gatgctgttt tgtatcggta
    17821 ttgcgcttat ttttggctgg cgcttaatgc gcgatgtaac cggtccgatt cgcaacatgg
    17881 tgaataccgt cgaccgcatc cgtcgcgggc aactcgacag ccgggtggaa ggatttatgc
    17941 tcggcgagct ggatatgctg aaaaacggta tcaactcgat ggcaatgtcg ctggcggctt
    18001 atcacgaaga gatgcaacac aatatcgacc aggcgacgtc cgatctgcgt gagacgctgg
    18061 agcaaatgga gattcagaac gttgagttgg atctggcgaa aaagcgcgcc caggaagcgg
    18121 cgcgtattaa atccgagttt ctggcaaata tgtcacacga gctgcgtaca ccactgaatg
    18181 gtgttattgg ctttacccgc ctgacgctga aaacagaatt aacaccaacg cagcgcgatc
    18241 acctgaatac gattgaacgt tcggcaaata atttgctggc aattattaat gatgttctcg
    18301 acttctcgaa actggaagcc ggtaagctga ttctggaaag tattccattc ccactacgca
    18361 gcacgctgga tgaagtcgtt accctgctgg cacattcttc tcacgataaa gggctggaac
    18421 tgacgctcaa tattaaaagc gacgtgcctg ataacgtgat cggcgaccca ctacgattac
    18481 agcaaatcat cactaacctg gtagggaatg caattaaatt caccgagaat ggcaacatcg
    18541 atattctggt agaaaaacgt gcgctgagta ataccaaagt gcagattgaa gtgcagattc
    18601 gggataccgg cattggtatt cctgaacgcg atcaatcgcg cttattccag gccttcagac
    18661 aggctgatgc cagtatttcc cgccgtcatg gtggcaccgg gctggggctg gtgattacac
    18721 aaaaactggt taatgaaatg ggcggcgata tttcgttcca tagccagccg aatcgcggtt
    18781 caactttctg gttccacatt aatctcgatc tgaacccgaa cattattatc gaagggccat
    18841 ccacccagtg cctcgctggt aagcgcctgg cctatgtcga accaaactcc gcagcagcac
    18901 aatgcacgct ggatatttta agtgaaacgc cgctggaagt ggtttatagc ccaacgttct
    18961 ccgcgctgcc tcccgcgcat tacgacatga tgttattagg catagcggtg accttccgcg
    19021 agccgctaac aatgcaacat gagcgattag cgaaagcagt atcgatgacc gatttcctga
    19081 tgctggcact tccttgccat gcacaagtca atgctgaaaa acttaagcaa gatggtatcg
    19141 gcgcgtgtct gctgaaacca ttaacaccta cgcgcctgtt gcctgccctg acggaatttt
    19201 gtcatcacaa acaaaacacg cttttgcctg taaccgatga aagtaagctg gcaatgacag
    19261 tcatggcggt tgatgacaac cccgctaacc tgaaacttat cggcgcattg ctggaagata
    19321 tggtgcaaca tgtggaactt tgcgatagcg ggcatcaggc ggttgaacgg gcgaaacaga
    19381 tgccgttcga tttaatctta atggatattc aaatgccgga catggacggc attcgggcat
    19441 gtgagctcat ccaccagctc ccgcatcagc agcaaacgcc ggttatcgcg gtaacggcgc
    19501 atgcgatggc cgggcaaaaa gagaaactgc ttggcgcggg gatgagtgat tatctggcca
    19561 aaccgatcga agaagagcga ttgcataatt tgctgttgcg ctacaagcct ggcagcggta
    19621 tttcctctcg cgtcgtgacg cccgaagtca acgaaattgt ggtcaacccg aatgcgaccc
    19681 tcgactggca actggcacta cgccaggcag caggaaaaac cgatttagcg cgcgatatgc
    19741 tgcaaatgtt actcgatttc ctgcctgaag ttcgcaacaa agttgaggaa cagctggttg
    19801 gagaaaaccc ggaaggcctg gttgatttga ttcataaact gcatggcagt tgcggctata
    19861 gcggtgtgcc gcgtatgaag aatatctgcc aactgatcga acaacagcta cgtagcggta
    19921 ctaaagaaga agatttggag ccggagctgc tggaactgtt agacgagatg gataatgtag
    19981 cgcgcgaagc cagcaaaatt ctcgggtaac ggacgtcttg tcgagtcaaa ataggttccg
    20041 tccaatgcca atgctgggag cagcacagac agtcccctcg cccctccggg gagagggtta
    20101 gggtgagggg aacaggccaa cgcaagtgcg aatgattacc ctcaccccgg tcctctcctt
    20161 gaaagggcga ggggaaaacc gtccccgtaa gccacttcgt cagtaatctc aaaccggctc
    20221 ctgaaagagc cggttttggt tttctgtctt aacgcaccat gcacggacgc ttgttatcga
    20281 acgtccagcc ggggatcagg tactgcattc ccatcgcatc gtcacgcgcg ccaagcccgt
    20341 gtttctgata cagctcgtgc gctttcatca cttgatccat atcgatttct acacccagcc
    20401 ccggtttttc tggcacctga accagaccgc ctttgatctc taacggttct ttggtcaggc
    20461 gctgattacc ttcctgccag atccagtgcg tatcaatcgc ggtgatttta cccggtgcag
    20521 cagcggctac atgggtaaac atcgccaggg aaatatcgaa gtggttgtta gagtgtgacc
    20581 cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct tgcatcgtcc
    20641 agaaatgcgg atccgccagc gggatatcaa cggattgcag ggagagcgta tggcccatct
    20701 gccgccagtc ggtagcgatc atattggttg cggtcggcag gccagtcgcg cgacggaact
    20761 ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct tctgcataag
    20821 ccagcgaacc tttcaggtat gtaccaattt taatcgcttc gttcagcgac caggcaccgt
    20881 taggatcgag ggtaatacgc gcctgtggga agcgttgcgc cagtgccaca atagattcgg
    20941 cttcttcttc cccggccagt acaccgcctt tcagtttgaa atcgttgaag ccatattttt
    21001 catacgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct tcatgacgca
    21061 ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagcggt gtggctttgc
    21121 gattaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt tgaccatcgc
    21181 ccagcagcga tgccacgttt acccccagat gctgccccaa cagatccagc attgccgctt
    21241 ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa ccgcgcccac
    21301 cggcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg tattcaccca
    21361 gcgttttacc caccaccagc ggaatcgcat cttccagcgt tttacggatt ttctcgccgc
    21421 cgggaatttc ccctacgcca gtgtgaccgg aattatcttt gataatcaca atattacgcg
    21481 tgaagaacgg tgcatgtgca ccactcagat tcatcagcat actgtcatga cccgccaccg
    21541 ggatgacctg catttcagta acaacaggcg tcgtaaattg agaactcata tttatgtcct
    21601 ttttcagaat caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc agccggggat
    21661 caggtactgc atcgggcctg cgtcgttacg cgcaccgcca ggcagacgtt tataggcctc
    21721 atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg catcaggaac
    21781 ggcaattttt ccgtttttaa tctccagcgg atttttggtc agacgacaat cgccctcctg
    21841 ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc ccacatgggt
    21901 aaacatcgcc agagagatat caaagtggtt attagaatgg cagccccagg tcagccccca
    21961 gtcgtcgcaa agttgcgcca cgcggactgc accggaaagc gtccagaagt gcggatcggc
    22021 aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc agttggtggc
    22081 gatcatgttg gtcgcgacgg gcaagccggt cgcccgtcga aactccgcca tcacttcacg
    22141 accggagaaa ccctgctcag cgccgcacgg gtcttccgca taggtaagga catcattcag
    22201 ccctttgcac agagaaatgg cttcatcaag cagccaggca ccattgggat caacggtaat
    22261 ccgcgcatca gggaagcgtt tcttcaatgc acgaacagta tcgatttctt gctcgccagg
    22321 taacacgcca cccttaagtt tgaaatcttt aaagccatag cgatcctgtg aggcttccgc
    22381 cagacgcaca acggcttcgc tgttcatcgc tttctgatgc cgcaactgat accactcatg
    22441 gttgcccggc gtattttcca gataaggaag atcggttttg gtccgatcac cgatataaaa
    22501 cagataaccg agaacggtaa tagcatcacg ttgcttgcct ggccctaaca gttcgcagac
    22561 cggaacattc agcgccgcca cggcattaac gcgcaattca aaagtccagg cacctttgcc
    22621 gaaggtatca aaatcggctg cctgattacc tttatgcacc tgctgaacca ctttattcag
    22681 gcgcgcaact tcctggccca gcaccatcgg aatagccttg accagtgttt gataaatcac
    22741 ctctccgccc ggcgcttcac caatgccggt atgcccggca ttatcggtga gtaccacaat
    22801 attgcgggtg aaatatgcgt tatgtgcacc accaatatta agcaacatgc tgtcatgtcc
    22861 ggcgaccgga atgactttca tatcagtaat aacaggactg gattgtgtcg tcatcttatt
    22921 gccccgcgac aggtttcagc tcaatacgct tgatatcgcc caccagcacc aggtagctga
    22981 gtaccgcgat taaggcatga acaccaacat aaatcagcgc cccattaaag gagccagtcg
    23041 tgccaacgat ataaccaatt gcgattggcg tgacgatgcc agaaatgtta ccaaacatgt
    23101 taaacaggcc accggaaaga ccgctgatct cttttggcgc agtatcagcc attacagccc
    23161 aacccagcgc accgatcccc ttaccgaaga aggccagcgc cataaagccg atgatcatcc
    23221 actcaacgtt gacgtagttg cagaacacca tcaccatcga caacaacatg cccatcacga
    23281 tcggtgtttt acgcgcaatg ttcagtgaac ccgtgcgacg catcagccag tcggaaataa
    23341 tcccacccag cacaccgccg ataaaaccgc aaaccgccgg aacggaagcc acaaagcccg
    23401 ctttcagaat cgacatcccg cgcgcctgca ccagataaac cgggaaccag gtaataaaaa
    23461 agtaagtcag ggcgttgata cagtactgac cgatataaac gccgatcatc atccgtgacc
    23521 cgagcagctg tttgatctgc ccccacttca cgctaaacgg cactttaact ttggtgtttt
    23581 gctgatccat attgatcagc gcgccacccg cggcgatgta ctccagctct ttctgattta
    23641 cgcccggatg ttgatttggc tcgtggatga ctttcaacca gataaagctg atgacaatgc
    23701 ccagaccgcc cataaagaaa aagacgtgtg accagcccac ttcatgcgtc agccagccca
    23761 taatcggcgc gaagataacc gttgcgaagt attgagcgga gttaaaaatc gacaccgccg
    23821 ttcccctttc ctgcgccgga aaccaggccg caacaatgcg actgttgccg gggaaagagg
    23881 gcgcttcagc aagcccgacc aggaagcgca gcgtaaacag ggcaacgata atgccgaatc
    23941 cactaaagat atcgacgaag ccttgcagca aggtaaacat cgaccagata aagatcgacc
    24001 agaagtagac gcgttttgaa ccaaaacgat ccagcaacca gccaccaggg atctgcccga
    24061 taacatacgc ccatgagaag gcagagaaca catagcccat tcccacggga tcaaggccga
    24121 tatctttggc catttccgaa ccggcaatag aaagcgtagc gcggtcgccg tagttgaagg
    24181 atgtgacgat aaacaacatc accactatcc agtaacgagc attcgtgcgt ttttccacac
    24241 tgctcgcagc ctgacttaaa gaactcattg ttgcactcct gaaaattcgc gttagccacg
    24301 ctcactctgg actgcgacat cgccaggaaa tcagaggtga cgtagggtgt ttttgccgtt
    24361 tttataggtc gttcgccgaa tacggcgcgt gtttatatct ggcaatagca gtataaaaag
    24421 cgcgccatag cggctcaccg tgcaacaaca caacattaat gcgttcaatg aggcccgatt
    24481 ttggcattag ccctggacgg tggaatccac ttcacggaaa tgaaaacaag aacaagaaag
    24541 gaagggttaa aacgaagaaa taaagaagag tatgaaatgg atcgcttgac tccaggcaaa
    24601 cgccagtaaa aatccgcgct atgaagcagt ttttactggc atttgcctga aaagattcga
    24661 ttcagcaccg ctaaaacggc atttaccgct cgctgaacat atcaggacaa catcgtgccc
    24721 caatgttcaa cccacggatt tgattccgtt tccggttccg ggttctcact ggcgtcaatc
    24781 aacagcattt cgccaacccg ctgagcgctc tgttcctgca acaaggcatc gaactgtttg
    24841 ccgccattgc agaaattcac ataactactg tcgccgagcg caatcacgcc ataacgcaga
    24901 ttcggctgga agcccagact atctttgatt ccctgaaaga gcggcacaat gctatcagga
    24961 aggtcgccct gcccggtcgt ggacgtaacc accagaacat acttatcctg ataaggcagc
    25021 cagtcgctta attcaggatc ttcaaatacc gttgctttgt ggccctgcgc ggtcagaatc
    25081 gcttccgctt cttcggccac taacagtgaa ttcccgtaca tggtgccgac aaaaatacca
    25141 atttccgcca tagcgttttc tccctggatt aggaacttat ctctccatcc tgacccgatg
    25201 gtgcactgaa ctcaaccctt tcattttcag gaagcagacc gcgccagcca aattgtgata
    25261 atgcctgcat ccaggtgtcg tccaggcccg cgtgaatagc cagtagctcg ccagtaaaag
    25321 gatgcgtcaa tgacaactga ctggcatgca gcattaaccg ctgaaggcca aaatgctcag
    25381 caccgctgcg attctggcgt aaatcaccat gtttgctgtc gccaataatc gggtgccgca
    25441 aatgggcaag atggcgacgg agctggtgct tgcgtccggt tttcggctcc agttccacca
    25501 ggccgtagcg cgtggtcggg taacgtccgg tcgctaccgg catttctacg gtcgccagac
    25561 cgcgataatg cgtcactgct ggctgcgggc ctttatcttc gcgggcaaat ttatcagcga
    25621 ttttgtccag ttcttccacc agtggataat ccagcaccgc ttcttccatc aaccagccac
    25681 gcacaatcgc atggtaacgt ttctggattt ggtgctgttc aaactgttgt gccagcagcc
    25741 gtccggcttc gctggataat cccatcaaca acacaccaga agttggtcgg tccagacgat
    25801 gagcagtaaa aacatgctgg cctatctggt cacgcacggt ttgcatgacc actactttct
    25861 cgtcgcgatc cagccagctg cggtgaacca gccagccgga gggtttattt accgcaacca
    25921 gccattcatc ctgatagagt atttccagca ttagctcgca tcatccgcaa aaagagcatc
    25981 cagtttctcc agctcagcca gaataagcgc gcgttgcgga tgatcagtcg ccagcgccat
    26041 ttcataatag ggcgcaacag caaaagcgcc cggtaacggc tggttgttat ctaacaaatc
    26101 gtgcattcgc gggatcagca cccactgcaa ccactccagt ggttccatgg tgtccataaa
    26161 gaacggctgg gtactcgcaa attgatgcgg caggggttca tcgtttcgcc agtgctgatg
    26221 ttcacgcagt aacgcttcaa gcgcctgcaa ctggagacga acgcggtcat gagtggtcat
    26281 aaagcaacct caataagaaa aactgccgcg aagaatagca ggaataaaaa aagggagcac
    26341 tgtattcaca gtgctcccgg ttcgtttcgc agcattccgg ctacttttat tgctccctgc
    26401 tcatccttga caacttttcc tctggccttg cggccaatcg ttcatcctga actattgctt
    26461 cctgctcaca ccaccccgat gtgatacttc atcctgaagt gttcctggcc ttcctgaccc
    26521 accgaatcat cctgaccggt tctcattctc catcctggag gtgtccttta acgcgtcctg
    26581 cgtcatcctc ttcgcttcat ccagaagcct ttccctgaaa caccatcctg gtgtgtcctt
    26641 cagaagtgtc atcatcctga tgttcacttc gttgtctgac tccctgtcga cgaagataga
    26701 atcgtctttt tctcgaagtc ttacaaggcg cttacagaca atgccttaag gaaaatttca
    26761 tatgaaagtt aagaattaac aagcaacatg ttgtttttaa atgagaataa cgattcttat
    26821 ctttttaagt attcgcttat ttcctggcga tctcctacag agtctgtaag agatctctca
    26881 caaccagatg gggtatttgg attacagaag aggcttaagt tgattaagga attccgcaag
    26941 atttgaggct aaatgcgtcc gtttacgtgt tcccagcgtc tctttgcaca cttcaccgga
    27001 gagattacac accgagatga cctctaactc ttcttccagc gtcgcgataa atagcgtggg
    27061 cggcagtttt agccttttct gagtcaccag atgaccaatc agattttcct gcacccgacg
    27121 gaagtcgtct tcactccaga tttgcagcaa tgtcagtttg atatcgccaa actgcgcgtg
    27181 catatccccg gcaaactgag tcgtataaaa ggtatgaatc gtggattgta tcacaatatc
    27241 aaaagcgcgt tcaaccgcgt ttacattttg ttccccggta aacggctggg gttgccagta
    27301 tacggcatct tcggtagttg aaataatgca cggtgaagga acgccgtaca gttcctcact
    27361 taacggccag cttttatgct cttcatgcca cgcatcgcag tagcgtgcag taaaatcttt
    27421 cagggctcgt gcggtcaaat cgtccactaa tttctctctt cacttaaacc agatacactt
    27481 gtcgtttagt ttatctggtt tatgacggtg aaacatgtct tcttatgcaa accatcaggc
    27541 acttgcgggc ctgacacttg gaaaatcaac cgattaccgg gatacctatg acgccagcct
    27601 actgcaaggc gttccacgca gcctgaatcg cgacccgctg ggtctgaaag cggataacct
    27661 gccttttcac ggtacggata tctggacgct gtatgaactt tcctggctga atgcgaaagg
    27721 tttgccgcag gtcgctatcg gtcatgttga acttgattac accagcgtaa atctgattga
    27781 gtcgaagagt tttaagctct atctcaacag ttttaaccag acgcgtttta ataactggga
    27841 tgaggtgcgc cagacgctgg agcgcgactt aagcacttgc gcccagggta aggttagcgt
    27901 ggcgttatat cgtcttgatg aactggaagg ccagccgata ggtcatttca atggcacctg
    27961 cattgatgac caggatatca ctatcgataa ctatgaattc actactgact atctggagaa
    28021 tgccaccagc ggtgaaaaag tagtggaaga gacgcttgtc agccacctgc tgaaatcaaa
    28081 ctgcctgatc acccatcaac cagattgggg ttcgatccaa attcagtatc gtggacgcca
    28141 aattgacaga gaaaaactgc tgcgttacct ggtctctttc cgtcatcaca acgagttcca
    28201 cgaacagtgc gtggaacgca tctttaatga cctgttacgc ttctgccagc cagaaaaatt
    28261 gagcgtttac gcacgttata cccgtcgtgg cggtctggac attaacccgt ggcgcagtaa
    28321 taacgatttt gtcccatcga ccacaagact ggttcggcaa taaatttttt ctcaattttg
    28381 cgtgctggat tcacgcagaa ggttgtgaaa ggtcagcagg cagggctatt gtaatcaaag
    28441 ggaatgacga tattcgtccc ataaggagtt tttcttgatt acacatatta gcccgcttgg
    28501 ctccatggat atgttgtcgc agctggaagt ggatatgctt aaacgcaccg ccagcagcga
    28561 cctctatcaa ctgtttcgca actgttcact tgccgtactg aactccggta gtttgaccga
    28621 taacagcaaa gaattgctgt ctcgttttga aaatttcgat attaacgtcc tgcgccgtga
    28681 acgcggcgta aagctggaac tgataaatcc cccggaagag gcttttgtcg atgggcgaat
    28741 tattcgcgct ttgcaggcca acttgttcgc ggttctgcgt gacattctct tcgtttatgg
    28801 gcaaattcat aacaccgttc gttttcccaa cctgaatctc gacaactccg tccacatcac
    28861 taacctggtc ttttccatct tgcgtaacgc tcgcgcgctg catgtcggtg aagcgccaaa
    28921 tatggtggtc tgctggggcg gtcactcaat taacgaaaac gagtatttgt atgcccgtcg
    28981 cgtcgggaac cagctgggcc tgcgtgagct gaatatctgc accggctgtg gtccgggagc
    29041 gatggaagcg ccgatgaaag gtgctgcggt cggacacgcg cagcagcgtt acaaagacag
    29101 tcgctttatt ggtatgacag agccgtcgat tatcgccgct gaaccgccta acccgctggt
    29161 caacgaattg atcatcatgc cagatatcga aaaacgtctg gaagcgtttg tccgtatcgc
    29221 tcacggtatc attatcttcc ctggcggtgt gggtacagca gaagagttgc tgtatttgct
    29281 gggaatttta atgaacccgg ccaacaaaga tcaggtttta ccattgatcc tcaccggccc
    29341 gaaagagagc gccgactact tccgcgtact ggacgagttt gtcgtgcata cgctgggtga
    29401 aaacgcgcgc cgccattacc gcatcatcat tgatgacgcc gctgaagtcg ctcgtcagat
    29461 gaaaaaatcg atgccgctgg tgaaagaaaa tcgccgcgac actggtgacg cttacagctt
    29521 taactggtca atgcgcattg cgccagattt gcaaatgccg tttgagccgt ctcacgagaa
    29581 tatggctaat ctgaagcttt acccggatca acccgttgaa gtgctggctg ccgacctgcg
    29641 ccgtgcgttc tccggtattg tggcgggtaa tgtaaaagaa gtcggtattc gcgccattga
    29701 agagtttggt ccttacaaaa tcaacggcga taaagagatt atgcgtcgta tggacgacct
    29761 gctacagggt tttgttgccc agcatcgtat gaagttgcca ggctcagcct acatcccttg
    29821 ctacgaaatc tgcacgtaat ctcccctccc ccggtgacgt ttgttgccgg gttttctcgt
    29881 ttttggtcgc ttactcatca actcatttca tttgttatat gaatgtttct taccaccctc
    29941 acgcgacaaa tatcaccaca gttaatatgt catacaattt atgttgaaac gcaaacgttt
    30001 ccctattttc ataaaccaca ttttatcgcc atgaaaaaga aattatcgcc atgattaact
    30061 aaaagtattg attttttcag ttcaacctac atatattgcg cgccccgtaa gaagtcagat
    30121 gtcgtttaat gggtaaatat tgcccttaaa ttctctttta cttttgattt acagagtaaa
    30181 gcgttgtgat tatctatctt ccaagtagat tattgtattt gggatcaaga tcactgatag
    30241 atacataact tgtgtgtatc tttccgccct caaattatta cggtggaaaa tgattaagcc
    30301 atcgccgata gacagatttc atttttacgg tcaggcacct tcccgggctg aactggctaa
    30361 aagctgaatt atttgcattc ctccaggaga aatagatgga aacgactcaa accagcacga
    30421 ttgcgtcgaa agactctcgt agtgcctggc gcaagacaga caccatgtgg atgctgggcc
    30481 tttacggcac ggcaatcggc gcgggcgtgc tgttcctgcc aatcaacgct ggtgttggcg
    30541 gtatgatccc gctgatcatc atggctatcc ttgcgttccc gatgacgttt tttgctcacc
    30601 gcggcctgac tcgcttcgta ctgtctggta aaaacccggg cgaagacatc accgaggttg
    30661 tagaagaaca ctttggtatt ggcgcaggta aactgattac tctgctctac ttcttcgcta
    30721 tctacccgat cctgctggtt tatagcgtgg caatcaccaa tacagttgaa agcttcatgt
    30781 ctcaccagct gggtatgacg ccaccgccgc gtgcgattct gtcgctgatc ctgatcgtgg
    30841 gtatgatgac catcgttcgc ttcggtgagc agatgatcgt taaagcgatg agtattctgg
    30901 tattcccgtt tgttggcgta ctgatgctgc tggctctgta cctgatcccg cagtggaacg
    30961 gcgcagcact ggaaacgctg tctctggaca ctgcatctgc aaccggaaac ggtctgtgga
    31021 tgacactgtg gctggcaatt ccggtaatgg tgttctcgtt caaccactct ccgatcatct
    31081 cttctttcgc cgttgcgaag cgtgaagagt acggcgatat ggcagaacag aaatgctcga
    31141 agatcctggc attcgcacac atcatgatgg tgctgaccgt aatgttcttc gtcttcagct
    31201 gcgtactgag cctgactccg gcagacctgg ctgcggctaa agagcagaac atctcgattc
    31261 tgtcttacct ggctaaccac tttaacgcgc cgatcatcgc gtggatggct ccgattatcg
    31321 cgattatcgc tatcaccaaa tccttcctcg gccactacct gggcgcacgt gaaggcttca
    31381 acggtatggt gattaaatct ctgcgtggta aaggtaagtc tatcgaaatc aacaagctga
    31441 accgtatcac tgcgctgttc atgctggtaa cgacctggat tgttgccacc ctgaacccga
    31501 gcatcctggg tatgattgaa accctgggcg gcccaatcat cgcgatgatc ctgttcctga
    31561 tgccgatgta cgcaattcag aaagtaccgg caatgcgtaa atacagcggt cacatcagca
    31621 acgtattcgt tgtcgtgatg ggtctgattg caatctccgc aatcttctac tctctgttca
    31681 gctaagtcct ttcgcgccgc tttcgggcgg cgcttcctcc gttttaacgc gatgtatttc
    31741 ctatgattag cgtattcgat attttcaaaa tcggcattgg cccttccagt tctcataccg
    31801 ttggaccaat gaaagcgggt aaacaattta ccgacgatct gattgcccgt aacctgctta
    31861 aagacgtgac ccgcgtggtg gttgacgtgt acggctcgct ctctctgacc ggtaaaggcc
    31921 accacacaga tatcgccata attatgggtc tggcgggtaa cctgccggat accgtggata
    31981 tcgattccat ccctggtttt attcaggatg tgaatactca tggtcgcctg atgctggcaa
    32041 acggtcagca tgaagtggag ttcccggttg atcagtgcat gaacttccat gccgacaacc
    32101 tttttctgca tgaaaacggt atgcgcatta ccgcgctggc gggcgataaa gtcgtttaca
    32161 gccagactta ttactctatc ggcggtggct ttattgttga tgaagaacat tttggtcagc
    32221 aggatagcgc accggttgaa gttccttatc cgtacagttc agcagccgat ctgcaaaaac
    32281 attgtcagga aaccgggctg tcactctctg gcctgatgat gaaaaacgag ctggcgctgc
    32341 acagcaaaga agagctggaa cagcacctgg cgaacgtctg ggaagtcatg cgtggcggta
    32401 ttgagcgcgg tatttctacc gaaggcgtgt tacctggcaa actgcgcgtt ccacgccgtg
    32461 ctgcggcact acgccggatg ctggtcagcc aggataaaac caccactgac ccgatggcgg
    32521 ttgttgactg gatcaacatg tttgcactgg cagtgaacga agagaatgct gctggcggtc
    32581 gcgtggtgac tgcgccgact aacggtgcgt gcgggattat tccggccgtg ctggcgtact
    32641 acgacaagtt tatccgcgaa gtgaacgcta actcactggc tcgttacctg ctggtagcca
    32701 gcgccattgg ttctctttat aagatgaacg cctcgatttc tggtgccgaa gtcggctgcc
    32761 agggtgaagt cggcgtggcg tgctcaatgg cggcagctgg tctggcagaa ctgttaggcg
    32821 caagcccggc gcaggtatgc atcgcggcgg aaatcgccat ggagcacaac ctcggtctga
    32881 cgtgtgaccc ggtcgccggg caggtacagg tgccgtgcat cgagcgtaac gccattgcgg
    32941 cagtgaaagc agtaaacgct gcacgtatgg cgctgcgccg taccagcgag ccacgcgtct
    33001 gcctcgacaa agttatcgaa accatgtacg aaacaggtaa agatatgaac gccaagtacc
    33061 gtgaaacgtc tcgcggcggc ctggcaatga aaatcgttgc ctgcgattaa tcgctctcca
    33121 gaggcctctt tttgcgaggc ctcttcccga tttctcatcc agccatagcc tgttctggca
    33181 tcgaatgtta cccttatcgc ctgatcttta agggagttat cgtggctgtt catttgctta
    33241 ttgtcgatgc actgaatctt attcgtcgca ttcatgccgt tcagggttcg ccctgtgtcg
    33301 aaacctgcca gcacgcgctc gatcagctca ttatgcacag ccagccaacc cacgcggtcg
    33361 ccgtttttga tgatgaaaac cgcagtagcg gctggcgtca tcagcgttta ccagattaca
    33421 aagcgggtcg accgccaatg ccggaagagt tgcacaacga gatgcctgca ttacgcgccg
    33481 cctttgagca acgcggcgtc ccgtgctggt ccgccagcgg caacgaagcc gatgacttag
    33541 ccgccacgct ggcggtcaaa gtgacacagg ccgggcatca ggcaacgatt gtttcgacag
    33601 ataaaggcta ctgtcagtta ctttcaccga cattacgtat tcgtgattac ttccagaaac
    33661 gttggctgga tgcgccattt atcgataaag aatttggcgt tcaaccgcag cagttgcccg
    33721 attactgggg gcttgcgggg atcagcagtt caaaggtacc gggtgttgca gggatcggac
    33781 caaaaagcgc cacacagcta ctggtcgagt ttcagagtct ggaagggata tatgagaatc
    33841 tggatgcggt tgccgaaaag tggcgcaaaa aattagaaac ccataaagag atggcgtttc
    33901 tgtgccgcga tattgcccgc ttacaaaccg atttgcatat cgacggcaat ttacagcaat
    33961 tgcggttggt aaggtaacgg catgccggat gcaaacgatt atcaacacat tgaatttcat
    34021 gcacttatat gccggataag acgcggctag cgtcgcatcc ggcattatca catcagcgta
    34081 tttaccaggc ggtatggtaa agctctacaa tatcctcaag cgttgcttca cgcgggttgc
    34141 caccggtaca aacatcatcc agtgccgcct gcgccagtgc cggaatgtct tccttgcgta
    34201 caccaacatc acgtaaatgt ggcggaatac cgacatcacg gttgagagca aacaccgctt
    34261 caacagcggc attacgcgcc tcttccaggc tcataccttc cactttcacg cccataacgc
    34321 gcgcgatatc gcggtacttc tcaccggtaa agtcagcgtt atagcgcatg acatgcggta
    34381 acaggatggc atttgcaaca ccgtgtggcg tgttgtaaaa cgcgcccagt ggatgcgcca
    34441 taccatgcac caaccctaac ccaacattcg agaatcccat acccgcaaca tactgcccga
    34501 gcgccatttc ttctccggca tccttatcac cagcaaccga tcctcgcagc gccccagcaa
    34561 tgatttcaat cgctttaatg tgcagcgcat cggttagcgc ccacgcgcca cgggtaatat
    34621 acccctcaat agcatgagtg agcgcatcga cacccgtcgc ggctttcagc gctggaggca
    34681 taccatccat catgtcagcg tcaataaacg ccacctgcgg gatatcatgc ggatcaacgc
    34741 aaacaaactt gcgccgtttc tcttcgtcag tgatcacgta gttaatggtc acttctgccg
    34801 cagtgcctgc tgtggtgggg attgccagaa tcggtacact gggtttattg gtcggggaaa
    34861 gcccttccag gctacgcaca tcggcaaact ccgggttgtt gctgataatg ccaatcgctt
    34921 tacacgtatc ctgtggagaa ccaccaccaa tagcgatcag gtaatccgcg ccgctattct
    34981 ggaatacatc gagcccttct ttgacgacag taattgttgg gttaggtact acgccgtcgt
    35041 aaatcgccca tgccagtcct gcggcatcca tcttatcgat cactttcgcc accacgccgc
    35101 attgcaccag cgttttatcg gtgacgatca gcgccttctg ataaccacgg cgtttcactt
    35161 catcggttaa agctccaaca gcacctcgac caaaccatgc cgtttcgttc agaatcattc
    35221 tgttagccat catccttctc cttgttgctt tacgaaatta ctcttcaatc cgtaacccgt
    35281 aggttttgaa tttctccagc actacggcaa tctcttcatc gctcagcact ggcaccgggt
    35341 ccgtaatcgc cagggtcgtc aggtaaagtt gtgccagcac ttcaacttca tgcgccagcc
    35401 ataacgcttt ctccagattc gcctcacaag cgataagccc atgatgttgt aacaaagttg
    35461 ccttacgatt tttgagagcc agcgcaacat gttcagaaag ttcgcgtgtt ccaaaggtcg
    35521 cataaggcgc gcaaggaata gaattaccgc cagccgccgc aatcatgtag tgaatagcgg
    35581 ggatcggtcg gttaagaatg gaaactgccg tacaatgaac ggcatgattg tgaacaaccg
    35641 cgttggcatc cggtctgctt tgataggccg ccatatggaa acgccactcg cttgagggga
    35701 gctttccttc ctcatgtttg ccgttgccat caataaagac aatatgcgac tccgtcagtt
    35761 tttcatatgg aatacctgtg ggcgtaatca gcatcccatc ctgataacgt acactaacgt
    35821 tacccgctgt cccctggttc agccccaggc gagtcatttc caggcaagtg tcaataatct
    35881 gacgagcaag tttatttcgt tccattagct acctctctct gattcaaaac agggcaataa
    35941 tgttgttcct ttcacactat tgaattagcc gtttaattac ccaccatctt cttcctgatt
    36001 aaccagaaag aaattcacaa gcttatattt tgtgacctgg ttcaactaat cacagtaaat
    36061 aactgcaagt tctcttttta taaccccatt aaaaatgacc gctcttaaaa atatttatca
    36121 aaacggtca
//
LOCUS       DAZFFE010000035        35420 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFFE010000035 DAZFFE010000000
VERSION     DAZFFE010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.035"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..443)
                     /locus_tag="ACHJ69_001976"
     CDS             complement(<1..443)
                     /locus_tag="ACHJ69_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO5014965.1"
                     /translation="MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVDIEYVFVAEKIVQYAEKNLEKNLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLDEIKALYKAEYAISRDALTIINEQFSVQLP"
     gene            complement(700..1953)
                     /gene="amiC"
                     /locus_tag="ACHJ69_001977"
     CDS             complement(700..1953)
                     /gene="amiC"
                     /locus_tag="ACHJ69_001977"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO5014966.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLTRRG"
     gene            2185..3516
                     /gene="argA"
                     /locus_tag="ACHJ69_001978"
     CDS             2185..3516
                     /gene="argA"
                     /locus_tag="ACHJ69_001978"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO5014967.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAQTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3578..5404)
                     /gene="recD"
                     /locus_tag="ACHJ69_001979"
     CDS             complement(3578..5404)
                     /gene="recD"
                     /locus_tag="ACHJ69_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO5014968.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     SHVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(5404..8946)
                     /gene="recB"
                     /locus_tag="ACHJ69_001980"
     CDS             complement(5404..8946)
                     /gene="recB"
                     /locus_tag="ACHJ69_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO5014969.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYARLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLTREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWQMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCNDDIAWQTAQIGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFEPQWEPVLTEWITTVLLAPLNETGISLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYATRPNAGLIDLMDEMFASMTLEEA"
     gene            complement(8939..11827)
                     /gene="ptrA"
                     /locus_tag="ACHJ69_001981"
     CDS             complement(8939..11827)
                     /gene="ptrA"
                     /locus_tag="ACHJ69_001981"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO5014970.1"
                     /translation="MPRSIWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQEG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQKKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARHVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAIFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRTMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(12003..15371)
                     /gene="recC"
                     /locus_tag="ACHJ69_001982"
     CDS             complement(12003..15371)
                     /gene="recC"
                     /locus_tag="ACHJ69_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO5014971.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTDELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLSS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCHHTRMPFDPQNYQPGN
                     LQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNVLVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15384..15707)
                     /locus_tag="ACHJ69_001983"
     CDS             complement(15384..15707)
                     /locus_tag="ACHJ69_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO5014972.1"
                     /translation="MSASLRNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15692..16099)
                     /locus_tag="ACHJ69_001984"
     CDS             complement(15692..16099)
                     /locus_tag="ACHJ69_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO5014973.1"
                     /translation="MNREKGVSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTQPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(16096..16659)
                     /locus_tag="ACHJ69_001985"
     CDS             complement(16096..16659)
                     /locus_tag="ACHJ69_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO5014974.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTNTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMHAASKADPQTVVDASYSVTGSNL"
     gene            complement(16650..17120)
                     /locus_tag="ACHJ69_001986"
     CDS             complement(16650..17120)
                     /locus_tag="ACHJ69_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO5014975.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISLIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17305..18099)
                     /gene="thyA"
                     /locus_tag="ACHJ69_001987"
     CDS             complement(17305..18099)
                     /gene="thyA"
                     /locus_tag="ACHJ69_001987"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO5014976.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNISSYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(18106..18981)
                     /gene="lgt"
                     /locus_tag="ACHJ69_001988"
     CDS             complement(18106..18981)
                     /gene="lgt"
                     /locus_tag="ACHJ69_001988"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO5014977.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19132..21378)
                     /gene="ptsP"
                     /locus_tag="ACHJ69_001989"
     CDS             complement(19132..21378)
                     /gene="ptsP"
                     /locus_tag="ACHJ69_001989"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO5014978.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21391..21921)
                     /gene="rppH"
                     /locus_tag="ACHJ69_001990"
     CDS             complement(21391..21921)
                     /gene="rppH"
                     /locus_tag="ACHJ69_001990"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO5014979.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(22274..22420)
                     /gene="ygdT"
                     /locus_tag="ACHJ69_001991"
     CDS             complement(22274..22420)
                     /gene="ygdT"
                     /locus_tag="ACHJ69_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO5014980.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            22606..23295
                     /gene="mutH"
                     /locus_tag="ACHJ69_001992"
     CDS             22606..23295
                     /gene="mutH"
                     /locus_tag="ACHJ69_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO5014981.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAKRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQIERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            23364..24077
                     /locus_tag="ACHJ69_001993"
     CDS             23364..24077
                     /locus_tag="ACHJ69_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO5014982.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            24215..24433
                     /gene="ygdR"
                     /locus_tag="ACHJ69_001994"
     CDS             24215..24433
                     /gene="ygdR"
                     /locus_tag="ACHJ69_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO5014983.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            24541..25581
                     /locus_tag="ACHJ69_001995"
     CDS             24541..25581
                     /locus_tag="ACHJ69_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO5014984.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25613..26806)
                     /gene="lplT"
                     /locus_tag="ACHJ69_001996"
     CDS             complement(25613..26806)
                     /gene="lplT"
                     /locus_tag="ACHJ69_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO5014985.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHILVALAVCALAYGGAVVANIYIPKLAAARPGQSWNLISMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26799..28958)
                     /gene="aas"
                     /locus_tag="ACHJ69_001997"
     CDS             complement(26799..28958)
                     /gene="aas"
                     /locus_tag="ACHJ69_001997"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO5014986.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTKALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            29544..30575
                     /gene="galR"
                     /locus_tag="ACHJ69_001998"
     CDS             29544..30575
                     /gene="galR"
                     /locus_tag="ACHJ69_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO5014987.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETIGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(30582..31844)
                     /gene="lysA"
                     /locus_tag="ACHJ69_001999"
     CDS             complement(30582..31844)
                     /gene="lysA"
                     /locus_tag="ACHJ69_001999"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO5014988.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDMVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     AGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAAEGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31966..32901
                     /gene="lysR"
                     /locus_tag="ACHJ69_002000"
     CDS             31966..32901
                     /gene="lysR"
                     /locus_tag="ACHJ69_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO5014989.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERIRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPRLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFHSENYISLSRTDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32888..33580)
                     /gene="ygeA"
                     /locus_tag="ACHJ69_002001"
     CDS             complement(32888..33580)
                     /gene="ygeA"
                     /locus_tag="ACHJ69_002001"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO5014990.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33709..35127)
                     /gene="araE"
                     /locus_tag="ACHJ69_002002"
     CDS             complement(33709..35127)
                     /gene="araE"
                     /locus_tag="ACHJ69_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO5014991.1"
                     /translation="MVTINTESALTPRPLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8776 a         9634 c         8682 g         8328 t
ORIGIN      
        1 tcaggaagtt gaacgctgaa ttgctcatta atgatagtta atgcatcgcg acttatcgca
       61 tactctgctt tgtacaacgc tttgatttca tcaaggaaaa cattattaat ttgtatacct
      121 gaaacgactc gggatattgc attcgaaatg tgatccgcaa gaatcaatag tagcgatgga
      181 ttgagatttt tttcgagatt tttctccgca tattgcacta ttttttcggc aacaaataca
      241 tactcaatat ctacatgttc aatcaattta taaagtttgt ttttttgttc atttctgaca
      301 taaaaaatcc ggttagccgg atgctcaggg accgacattc catatttttt gttgtaaccg
      361 acgccaggcc cagaaatgat aacctcctgt ccattcatcg atgcctgtac acaattattg
      421 ttcatgactt tttcgatgat cattccatgg ctccaaaaaa aaagcaaggc acgtcagaag
      481 taataactac ttcaaacatt gccttgcctg atttaacagt tacaagatga ttaactaatg
      541 cgtatacatt acacggaatt gtgcttaatt aaagagatgg tcggcttatt tttcaagccg
      601 acattcaaat atccgacatc agtcacaaaa ccagcgtcta atattcaggt aaatgttatc
      661 tattcaacga aatataaatt cattgaataa ataaaaacat tatccccttc tcgtcagcgt
      721 cgccccatcg gcaaaatacg ctttaattcc cgcaagaata gactccgcaa cttcctgctg
      781 gaaagtcgcc gttttcagtt tacgctcttc ctcaacgtta ctgataaacg ccgtttcgac
      841 cagaatggag ggaatatctg gtgcctttag tacggcaaac ccggcctgtt caacttgatt
      901 tttatgcagc ttgttgattt taccgagctt attcagcacc gctttaccaa acttaaggct
      961 gtcggcaatg gtcagcgact gcaccatatc gaacatggtg tggtcgacat agcggtcacc
     1021 gcttttgctt acgccaccaa tcaagtccga ggcgttctgg gtttgtgcca gatattttgc
     1081 cgcagtactg gttgcgcctt tggttgagag cgcaaacacc gaggaaccgc tcggctggcg
     1141 actggtaaag gcgtcggcat ggatagaaac aaacaagtca gcgcgctgtt tctgggcttt
     1201 tgctacgcgc acttgcaacg gaatgaagat gtcttcattg cgcgtcatgt acaccttcat
     1261 attgccctct ttctcgatca gagagcgcag acggcgagct atttgcaata ccacgtcttt
     1321 ttcgcgcgtt ttgtatttcc ccaccgcacc ggagtcttcg ccaccgtggc cagggtcaag
     1381 cataatgaca atcggacgat cccgcccagc tttacccggt tgtggaccac tttgtgctgg
     1441 cggcacctgc ttttcgaggt cgcctttgtt gtaatcctcc agcagcgcca gcagcgggtc
     1501 ctgcatatcc tgtgcattgg ccggatagag gtccatcacc agacgctctt taaacccggc
     1561 gaccggcgca agggcaaaca gctgcggttt tacgttttgc tttaattcaa aaaccatacg
     1621 tacggtttgc gggtcaaatt gcccgacgcg cgccgacttg atgaacgggt cgtcagcgcg
     1681 gatttgcgca gccatcccct tgagcaccga gttcaggttt acatcttcga tatccaccac
     1741 cacgcgttca ggattactca acgcgaactg cttatatttc agctgacgat ttgattctac
     1801 cgtcacgcgg gtgtagctgg acgcaggcca gacgcgcacc gccacgacct ggctgaccgc
     1861 agccaggctg acctgactta cgctcaatag ccacatggca cccgcgcctt gcagtaaacg
     1921 acgacggctg attgcagtgt tggatcctga catgcctctc ccgagcaaaa gaaatctaag
     1981 ctgtgtaaca agtaaacgac caatttgacc ggaaacttta gcgaaagacg cataatctgt
     2041 catctataaa acggtaaaca ttctttttat attcacggta ttactgataa aaaactcgct
     2101 cccgcataaa atttacactt gcaccccgcg aaaaaacaga ataaaaatac aataatttcg
     2161 aataatcatg caaagaggtg taccgtggta aaggaacgta aaaccgagct ggtcgaggga
     2221 ttccgccatt cggttcccta tatcaatacc caccggggaa aaacgtttgt catcatgctc
     2281 ggcggtgaag ccattgagca tgagaatttc tccagtatcg ttaatgatat cgggttgttg
     2341 cacagcctcg gcatccgtct ggtggtggtc tatggcgcac gtccgcagat cgacgcaaat
     2401 ctggctgcac atcaccacga accgctgtat cacaagaata tacgtgtgac cgacgcccaa
     2461 acgctggaac tggtgaagca ggctgcggga acattgcaac tggatattac tgctcgcctg
     2521 tcgatgagtc tcaataacac accgctgcag ggcgcgcata tcaacgtcgt cagtggtaat
     2581 tttattattg cccagccgct gggcgtcgat gacggcgtgg attactgtca tagcgggcgt
     2641 atccggcgga ttgatgaaga cgcgatccat cgtcaactgg acagcggtgc aatagtgcta
     2701 atggggccgg tcgctgtttc ggtcactggc gagagcttta atctgacctc ggaagagatt
     2761 gccactcaac tggccatcaa actgaaagct gaaaaaatga ttggtttttg ctcttcccag
     2821 ggcgtcacta atgacgacgg tgatattgtc tccgaacttt tccctaacga agcgcaagca
     2881 cgggtagaag cccaggaaga gaaaggcgat tacaactccg gtacggtgcg ctttttgcgt
     2941 ggcgcagtga aagcctgccg cagcggcgtg cgtcgctgtc atttaatcag ttatcaggaa
     3001 gatggcgcgc tgttgcaaga gttgttctca cgcgacggta tcggtacgca gattgtgatg
     3061 gaaagcgccg agcagattcg tcgcgcaaca atcaacgata ttggcggtat tctggagttg
     3121 attcgcccgc tggagcaaca aggtattctg gtacgccgtt ctcgcgagca gctggagatg
     3181 gaaatcgaca aattcaccat tattcagcgc gataacacga ctattgcctg cgccgcgctc
     3241 tatccgttcc cggaagagaa gattggggaa atggcctgtg tggcagttca cccggattac
     3301 cgcagttcat cacggggcga ggttctgctg gaacgcattg ccgctcaggc gaagcagagc
     3361 ggcttaagca aattgtttgt gctgaccacg cgcagtattc actggttcca ggaacgtgga
     3421 tttaccccag tggatattga tttactgccc gagagcaaaa agcagttgta caactaccag
     3481 cgtaaatcca aagttttgat ggcggattta gggtaagcga tgaaaatcgc cggatgcgac
     3541 atgcgtaaca ctcgtgcgtc gcatcaggca attacgttta tccccgtgaa ctaaacaacg
     3601 ccgccagacc actgcgccgc tcagtacgag tggcgattgc cgcacttaat atgcgctcat
     3661 cggcatacag cgacagacgg cgacgcgcgc gggtcaccgc ggtgtaaacc agctctcgcg
     3721 ttactaccgg cgtgcgttga ctcggtaaaa tcaacgccgc atggtcgaac tccgatccct
     3781 gcgatttatg taccgtcatc gcccacgtcg tttcgtgctc tggcaggcga ctcggttgca
     3841 cagacttaat attgccgtcc ggcatcgcaa accagacgcg cgtcccctgc ccgcgatcca
     3901 gcgcaatacc aatatcgcca ttaaacaacc caagcgcgct gtcattacgg gcaatcatca
     3961 ccggtcggcc ttcgtaccaa cgagagtgcg gatgacgatg aattttgcgc ttctgttgca
     4021 taaactgctc aattcgctca ttcagtccag ccacgccaaa cggcccttcc cgcagggcgc
     4081 acaaaagctg gtactcattg aacgcctgaa tgattaaatc cggctcggca cgcgcttgca
     4141 gcagatccag ataacgtccg taacccgcaa gagcttcctc aagcatcgca atataatctt
     4201 cgccgctctg taaaagtcgt ttttcgatat cagtaaaatc ctgctgaaaa acggttttca
     4261 ctgccgtttt atcaccacgg ttaatcgccg cagctaactg accaatgcca gaatcgctgc
     4321 cgaaacgata gcttttttgc agcaggcaga ggctgtcgcg caaagatgcc gcttctgtgc
     4381 cagttcctgc tggaacgtgg cttcccgtca ggcggctcaa ctgcccggca cgctcggcgg
     4441 taaagcccgc gttggcataa gcgcagatat cgcccagcac agccccagcc tcaaccgagg
     4501 ccagttgatc acgatcgccg agaaagatca ctcgcgcatg atcgggcaag gcgtcgatca
     4561 gtctcgacat cataggaaga tcgatcattg acgcttcatc taccaccagc acatcaagat
     4621 gcagcgggtt accggcatga tgacgtaaac gctggctacc cggctgcgca cccagcaatc
     4681 ggtgcaaagt gctggcatct tccggaatgc gtttcttttg ttcatcggtc agcggtaatt
     4741 gtcgcaaagc cttgccgaga gattcggtta agcgcgcggc agctttaccc gttggtgcag
     4801 ccagacggat acggcagcgt tcgccgtcgg ccatctgaat taaggctgcc agcaacttcg
     4861 ctacggtggt cgttttaccg gtgccaggac cgccggaaat caccgaaatc cgccgcgtca
     4921 gcgccactgc cgccgcaact ttttgccagt taatttcatc gcttactgga aaaagtttgt
     4981 ccagggtttg cgccagtaga gcttcatcaa cctcaatggc atgattcact tcgttgaaaa
     5041 agcgtgccac tgtgcgctcg ttacaccaca tgcgattcaa ataaagacga tcgccacaga
     5101 ggatcatcgg cgttggttca tctccccggc tgaccgcttg tgaagccagc aagcattctt
     5161 cccaattttg tagttcaccg atttcactga cacaggtcgc caacagcgga tgcgatgcct
     5221 cgttattttc cagtcgtgaa agcggcaaac aaacgtgtcc ctctccggca tcatgactta
     5281 atagtgccgc cgcgagggtg acggcaggat gttcatctcc cgccacggtc agggcaaact
     5341 gcacgtccag cgggcgtagc tgtttgtgct ccacagcttc cagtaattgc ttttgcaatt
     5401 tcattacgcc tcctccaggg tcatactggc aaacatctca tccatcaggt caatcaaccc
     5461 ggcgttgggt cgggtcgcgt agatcccttg ttgcggatgt tctttatcaa cgccacgcag
     5521 gaacagataa ataacgccgc caaagtggcg ctcatagtcg taatcagcaa tacgatggcg
     5581 cagataacgg tgcagcgcca gggtataaag ctgatattgc agatcatagc ggtgtgcctg
     5641 cattgccgct gccatagcct gttgggtgta agccgaactg tcttcaccca accagttgga
     5701 tttatagtcg agcaggtaat aacgcccttc gtggcggaac accagatcga taaagccttt
     5761 taacatgcca cgtacctgca tgaactccag cggcgggcag ccagcggata gcgggtcaaa
     5821 ctggcgaatt agtgcatcaa gctggctggc gatgagcggt tcgctaattg gcaggtaaaa
     5881 ctccatctcc acctgtttat cgcgatcgga aagctgactc aggctaatac ccgtttcatt
     5941 gagaggtgcc aggaggacag ttgtgatcca ctcagtcaac accggttccc actgcggttc
     6001 aaagccaccg agctccagtt tttcctgtac ccagttcggg tcaaccggct gggtaaaatc
     6061 gaggtcttca aacaaactgt gcaagaatgt ccccggtgac gcaccgcgcg ggaactgatg
     6121 cggtgttaac gtcggttctt caacgacgct gaccacgcct gcggcatcaa catccagccg
     6181 tggcatcaga tcctgagcga taccgtgacc acgctgctgc aaaccggagt agctggtgac
     6241 acgccagtta tcgccgggca atcgttgtag cgtcctcgca ttcagttctg cagttaatgc
     6301 atcattaacc tgccagggct ggttatcacc aatttgtgcc gtttgccagg caatatcatc
     6361 attgcacaac gcttcaatac aggtacgaag ccctgccgca tcttgcggtt ctcctttttg
     6421 cagcaaacgc ccgagcgcac tttggtggac gtcggtatca ccttttttat cgccacgacg
     6481 gcgcaccagc ggtgcaacgc cgagactgca atgccaaact gaacgcgtca gcgccacgta
     6541 aagtaaacgc agatcttccg ccagacgttc ggcctccgcg aggtcgacgc tttctggcgc
     6601 agcattaaga tccagaacgg cctcaaacga gtggcgatcg tgataaaacg cctgatcctg
     6661 gacgcggaaa ttggtaataa atggcaacca gaccagcgga tattccagcc cttttgattt
     6721 gtgtatcgtg acaatctgca ccagatgttt atcactttcg aggcgcattt gttggctgga
     6781 ggcattactg tctggctcga ggatatgttg cgataaccag cgtaccagcg catgttcact
     6841 ttccagctgc gttccggctt cttgtagcag ttcgctgata tgcaagatat cggtaagacg
     6901 ccgctcaccg cctgccgttg ccagcaagtt ttctgcaatg ttacgcgccg acatcagcgc
     6961 ccgcagcatc ggcataacgc cacgtttgcg ccagatttgc cgataaccat cgaactcttc
     7021 gacgaccgca tcccacgcat gttcgtcatt attcagcgtt tcaatatcca gcgcattcag
     7081 ccccatcatt gacgttgcca gcgcactgcg cagggtgttc tcacgttcgg gcgtcatcac
     7141 cgcctgcaac agccaaagca tctcctgtgc ttccagagtt tcaaaaacac tgtcgcggtt
     7201 cgaaaggtaa acggaaggga tttccagcag cgttaaggca tcgcgcacct gagcggcctc
     7261 ctggcggctg cgcaccagca cactgatgtc cgaagcacgc accggacgcg cgtcgtcgcc
     7321 gttcatcagc aacgcttcgc cgcgttgccc ggcttgtaac cagtcgcgga tttgcgcagc
     7381 acatacctgc gccatggtac tttgatagtc gccaacgccg cagctttcgc cttccatctg
     7441 ccacattttc atcgcaggct gcgtttcacc tttaaacaca aaacgtaacg cctgattttt
     7501 gccggcagat ttcacaggaa taaacggtat ttcacgaaac atgaacgcgt catcagtctg
     7561 gctgaaaagt ttgttaacgc tgttcaccat tcccggtgcg gaacgccagt tggtatctaa
     7621 ggtgtagtga gcatgaactt cgctacgcgc cttcatataa gtgaagatat ccgcaccccg
     7681 gaatgcatat atggcctgtt tcgggtcgcc aatcagcaac aatgcggttt ccggctgatg
     7741 gtgccagata cggcgaaaaa ttcgatactg ttgggggtcg gtatcctgaa attcatcgat
     7801 cattgccacc gggaatcgcg tacggatcgc cgctgccaat acctcgccgc tttcgctacg
     7861 cagcgcggaa tcgagccgac ttaacatgtc atcaaaaccc aattcgccac ggcggcgttt
     7921 ttcacgcgct actgtttcgc ggatctcagc caatgcgcgg gtgatcacca gatcgcggat
     7981 cgacaatggt tctgcaagca gttgatcgat cgcctcaaac agtggatgtc gcggggtttc
     8041 ccccccggct ttcgtgcgat cttctaagaa acgctgagag aatttttcca gcgactctgg
     8101 caactgataa ctgttggtct cttcttctgc ccaggcgctg atcttgtcga tccatttagc
     8161 ctgattgcta cggttaaact tgcgtcgatc aataccagaa gattcgatca gcgcatccag
     8221 ttcacccacc gcgtcgcgcc actgctgttt tacggcgtcg atacgcgcca caatttgcgc
     8281 gtggcgggaa gccagcgttt catcatcggg cggcggtgct ttgataaccg gtgcttcgcc
     8341 ttgcagataa cgattaatat cgcgcagcaa cgcctgcggc cctttccagg tttcaaagac
     8401 cacctgggca atttcacgcg tcagcgggta gcagtggcga cgccagaaat cggcgcaggc
     8461 ctggtagcgt agcagagact catcttcaat cagctgctgc tcaaacagca tgccggattc
     8521 aaaggcattc aggttgagca tgcgctggca aaaaccgtga atagtaaaga ctgccgcttc
     8581 atccatctgc cgttcggcta acaacaacca ctgcgcggct tgcgctttat cgtcgatctc
     8641 ttccagcaga cgtgcataca gcggattgtc agtggtttca cgcaggcagg cgatacgcaa
     8701 ctcgtgaata ttgctacgga tacgaccgcg caattctgcc gtggcagcct cggtaaaggt
     8761 gaccaccagc agttcttcaa cggtcagcgg gcggggaaag gcggcggaac cgcctagccc
     8821 aagtaacagg cgcaaataga gcgctgcaat cgtaaaggtt ttgcctgtgc cggcagaggc
     8881 ttcaatcagg cgctcaccct gtaagggcaa gcgcaaagga tctagtgtct cggcgacatc
     8941 actcattctt ttcactcatc aggggcattg tttgctgcaa cgcgctgacg ttctcccaca
     9001 ctttccagcc ttcaggatgc acatattcgg ctttcccgtt ctggctgccg gaaatctgcg
     9061 acagaatagc cataccttgc ggctcgacca ccgcctgatg gaagaaatca gcaagttttt
     9121 gcggcgtcag cagttttatc tgggccacga ttttatcacg cgaatcgaag cgcatattgc
     9181 cgcgatcgaa atctttactt aacttcgatg cttcttcgcc gagcgtttgc ggtgcctgca
     9241 gcatctgggt tattaccgcc tgctggattt gcgcaaactc ttctggcttc atcgtccgca
     9301 attttgcctc tgcggttggg aaaaacgcct tgtaacgctc ccacaagaat gaaggctgtt
     9361 tatcattgct ttgcaaaagg aagcccatgc cccactgacg ccccacgctc attggaaacg
     9421 caaacacggc atagcccaac tgttcttcgg tacgcaactg attgtagaac cacggctgta
     9481 cgatctgccc caacagagag ctataggctg agctggtgta ttcatcgtag ccagtcggta
     9541 caaatatcgc tgccagtgcg gagtcggtgc tgttaccagc tttttcaaag atgacggatt
     9601 gttttttatc gaccacgaca tctttgttac gacaccactc tgaaccatca gcgcccaact
     9661 gtttttgcac atggcgtgcc agcgttgttg cctgggcttc agtcatgttg ccgataacca
     9721 taaactctgg tctagcccct gattttaagg cgtcgcgata ggccagcacc tctttcaacg
     9781 taatggaggg caaaattttg cgccgttcat ctcgcgagaa gtacggcact tgcgagagca
     9841 tctgcgcggg cataatcgcc tgctcaaacg ctttgccctt ttctgcggaa tccatcatct
     9901 ggttatacca ggacttcgcc tgctcaagct gatcttccgt agcggtatag ctaaagtagc
     9961 cctccagcag agcctggaac agctgcggca ggcgctgagt gtaaccattg gcattaacca
    10021 taaggccgtt gttagcattg gtggaaaaac ttatgccacc aaccgacgcc tggttgctta
    10081 actgatcaag cgccagccct gcgagataat cattgagcgc aaacatcacc tgattgcggg
    10141 cgctgtccat ggctttcgga ttacgcaaaa tcaggctgac atcagctttg ggctcactgg
    10201 caaaataacg gcttggcgca tacaccacgc gcagattcga ctcatcaaca atcaactctg
    10261 gatggtcgta tttcttctct gacttaatca gcgagaaatc gtcaggaata taggggttaa
    10321 gctccggtaa ggagagcgca atattggcgg cttttttctg ccagtccgcg aaagtttgtt
    10381 cgctgatttt atcgacttga tacggcgcat cgacaaaata agccgtttta ttgtgcggct
    10441 ctttcgggct gatataccag atacgcgcat tctgcggcgt catcatcgcc agacgttctt
    10501 ttactgcttt agcatcgtac cgatcggcaa tattgactgc atccagtgta tgctcaacag
    10561 gaacgcgaat catggtatca gccagccatt cgacgtaatc catatcacgg gtgattgacg
    10621 gataacggaa gtcgatatcc agcacattcg ccagttcatc gaagtattgt ttatcgatcc
    10681 ctttttcacg taacagattg agatagctaa aaatggccgc cacaacctga tcgcgattcg
    10741 ccagaccttt atcggttaaa gacgcagaga tcgctaatac gccgctgttg ccgttgacga
    10801 taggatcgga attggcgcta ataccctcaa ctaatccctg cttttgcagc cagtcagaaa
    10861 gtgtaccagg gctgcgatta ccaatcagat aggtaatcaa ttcatccgtt ttactacgga
    10921 acttcgctga attgttatcg atgcgaaact caacgcgcag aactttacgc ggcaacgccg
    10981 ggacgtaatg aatgataatg ccctcttgcg cgtcggtgac taccggcacg gtgatttccg
    11041 gttttttgct ctctttgttc ggcacgcgac caaaggtgtc cgccgccatt ttcgccaact
    11101 ccggcaacgg tttattgctg taaataaccg ccttcatcag attggcggaa tagtacttct
    11161 cgtggaaatc tttcagcgcc tgctgtaccg gattacctgg tttgtcgctt aaagtttcga
    11221 ggttaccacc agaaaacttt gaaccggggt gtgccgggtt aatggtttct gcgctgacct
    11281 gtgccatgcg catcccgtca cgcgtacgcg ccatggttaa ttcggcattc acggcattac
    11341 gttcacgttc ggcgtatttc ttgtcgagca agggttctgc aatagcgtcc gccaggcggt
    11401 ctaccgcacc gggcaaggcg tcgttctcaa cttccagata gaaagccgtg cgatacggtg
    11461 ccgtgctggc attgtgactg ccgccgtgca ttttgagata ttcggccaga ctgtcagcct
    11521 gcggatactt tttcgacccc atcagactca tatgttcaag gtaatgtgcc agcccctggt
    11581 acgcctcagg atcttccagc gacccaacgg gcaccaccag cgccgagagc gatttaactg
    11641 cctgcggatc agaaaccagt aagaccacca taccgttatc cagacgtata gcctgatact
    11701 ggcggttatc tttatcactt ttacggatgg tttcctgaat cggctgccat cccgtttctg
    11761 cctgacttaa gggtgcccag agggcaacaa acaacaataa tgctttgaac cagatgctgc
    11821 ggggcattca cggacctcat aatcaacttt attttctggc cagattcaac acgttaacgc
    11881 aaatcatctg ccagaattta atcttgtgct gaacgagtca gcctatgtct atataaccat
    11941 cagtccgtga ctggtgcgca tcataaagta agcggataga ttgtgcaatt tttatacaat
    12001 actcatgact gattaaagcg aaacagcggt aacaggaaac gttgcgactg ttcaacgata
    12061 gcctccattg tctctggtgt taattgccgc cagagccgtt gataccagat atcatcacct
    12121 tcgccacgca ccatcatgtt gccttcgtaa gcctgaagga atttcgtacg ggctttttgc
    12181 aacgtggaat cgtcatccag catggcatcg ttttgcgcgt cataacaggt ttttagccac
    12241 gcgccgccac tttcaggtaa caccagcaat ggcgcggaca ttccttcacg atacccttca
    12301 atcagctgtg agaggtaatg taaagcctgt tcggctgcaa gcggcggaaa acgccactcg
    12361 ccgtctttgc gtagaaaaag gcgactttca ccattaccac cgctggcaca gtagacaagg
    12421 tgttccagcc aaagttgcat tccctgcgcc acacttaata aagagggacg ccagcgcaac
    12481 aggccatccg gctgcacctg cggcaaccag ccagttatct gcacaccgtt gcaggcgaga
    12541 tcgatttcca tgctctgccc tggctggcga caggcaatga ctctgtcggc aagttgctgc
    12601 atctcctgac actgcgtttc ccagaaaatt tcaccaaagg caccatacgg caaatcacct
    12661 gccgcccgga atcggcggaa caagcgttcg gcatcatcct gctcaaccag tacatttaat
    12721 aactgctggt tgatttgata acgactaagc ccttcaagaa taaaaggttc ggtgtcagga
    12781 atttcgctgt cttcggtacg gaagttcacc tgcaaacgca tctggaaaaa cgcccgcacc
    12841 ggatgtgccc agaatcgttg tagcgtctcc agcggcacag tttccggcaa ggtaaacggc
    12901 agcggctgaa caaattcaga atgtgcttta ccagcctggc tggccgcagg tagccactcg
    12961 cgcgcatagc tttgtaagtt accgggctgg tagttttgcg ggtcaaacgg catccgggta
    13021 tggtggcaag taagatgcgc ttttaccctt gcctcgcttt catcacagtt aagcgcttca
    13081 tcgcccggta gataatggct ttgcccgatg tagtcgatca gttcctgcac cagtaccgac
    13141 gggaaacgtt cactgttatc ctgaatggaa cgcccgatat agctgatata gagtttttgc
    13201 tgcgcggaaa ttaacgcttc caggaacaga tagcggtcgt catcgcgacg gctacggtcg
    13261 ccacgcttcg gtttctggct catcagatca aagcccaatg gcgcaagctg acgtggataa
    13321 acgccgtcgt tcattcccag caggcaaacc actttgaacg gaattgaacg cattggcatc
    13381 agagtacaaa tgttaaccgg tccggcgaga aaacgctggc tgatacgttc ttgatccagg
    13441 cgctgtgcca gttcatcacg caatagtgac agcggcaccg cgtcgccata ctgcgcacct
    13501 aaaccttcgg cgataatcgc ctgccattgt tgttcgatca gcgtcatcgc tgcttcggtt
    13561 tccgcatccg gcaggaagaa ggcgttgagc atatcgcgac aaaccggcaa ccactcttcc
    13621 agcggacgct cctgcgccag cccgcgacgc cagatgttta gctgcattag cagtgaagac
    13681 agatgcccca ccagttctgc aattaagccg ctcgattcat cataaggtag aaccgattgc
    13741 cactcgccct gcgcgctctc catcgcgtag cccagcaaca tgcgcgtcag gccaaaccgc
    13801 caggtgtgtt gaccggtagc gggaagttcc agctcgcgaa cgttgtcgtc atctatcccc
    13861 caacgaatgc cggattcgtt gacccactga cgtaaataac gcagcccttc ttcggtgatg
    13921 tcaaaccgcg ctgccagcac cggcacatcc agtaatgcca gcacgtcttc cgacacaaag
    13981 cggctgtctg gcagcgataa caggctgata aacgcttcaa gtacaggatg cgactgccgc
    14041 gcccgacggt cggaaatggc gtaaggcagg taacgatccg caggtgcact accaaacaca
    14101 gcctgaataa acggactgta gctgtcgata tcagccacca tcacgatgat gtcgcgcgga
    14161 gtaagtgtcg ggtcttcctc cagcatcgcc agcaggcgat cgtgtaaaac ttcaacttca
    14221 cgctgcgggc tatggcaaac gtggaaggtg atactgctat ccagtggatc aagcgggcgt
    14281 ttgttatcgc tacgggaaaa ctcttcgatg ttcacaccag caacggcgcg gttttccagt
    14341 tccagaatgt cagactgaat attatgcagc aggttatctg gcgtcacatc gacaaaagcg
    14401 tccagctcct ggctgctctc caggtcagaa aggagataaa tgtagtcgcg cccaagcttg
    14461 ccccatgaag ccagcagcgg gttgccgaca tcctgttcac catcgctgtt aaagagctgc
    14521 ccggcatttt cgctgtcgcg aaatagcggt aattcgcgat cttcaaaact gtgtcggcgc
    14581 tggcgagtca gtagtttcgc cagataagct ggatctttaa tgtcgcccca gtaataacgg
    14641 caggggttgg taaacaggag atggatttca atatgtttac ccagcgcctg tagcgcctgg
    14701 agataaacag gcggtaacgc ggaaataccg catataaaga cgcgcgaagg taaccccggc
    14761 gggcaggtcg tcgcggactc cagcgtttcg ataaagcgct gatagagatt ggcgcggtgc
    14821 cagcgcggtt gcccaagttc gtcggtatat tccaccaacg ccttccacaa cggcgcttgc
    14881 caggcctgtg cttctcccaa cccttctacc agatgtcctg tttcccactg tgccagccag
    14941 tccggacgat agaccagata ctggtcaaac aggtccgccg cttttgaaga aagctggaac
    15001 agttttcgct tgtcactatc gtcagtcaga taatgccgca acagggtaaa gtcttcgcgc
    15061 tccaacagtt gcggcagcag agtcatcagt ttccagctca tgctctgttt gttaaaggcg
    15121 ctctctttgg ggatctccgg taacacccgg acgaacatat cccagataaa gctcgctggc
    15181 agcggaaaat caatgtttgc cgcaatacca aacttttgcg acagggtcat ttgcagccac
    15241 tgtgccatac cggtactttg caccagaatc atctctggtt cgaaaggatc gtccagccgt
    15301 tcgcgttcga caataaactc catcaacgct tccagcacgt ccagacgatt ggaatggtag
    15361 acccttaaca tagcggctcc tgactactga cgattcgggc aatgcaggcg ggtcatctcg
    15421 ccttctctgc ccccgggtga aactagcgta acgctgatgc tgacacatcc cgcctgcgat
    15481 gtctgcattc ggttgacctg ccagttagca ggtggcgaaa tcgcgcgcag ttgcgtttgc
    15541 tgccagccat gccgccagag ctgttggtac tggtttctgc tggcaagact gttcattaat
    15601 gttcgctgga aacccgataa cgcagtgaca atcatcacca tcaacaccat cgccaacatt
    15661 acctccggca gactaaagcc ttgttgattc ctcagggaag ctgacataac gccctctctt
    15721 tcagcggaca aaaatcgctc cagccgcgtg gcgaaaaaac aatgtttcca tcgatgactt
    15781 cgcctgttcg ccacaacgaa acgccttcat aaccagcaat caataaggct tcattatctg
    15841 ccagtaaacg cagacaaacc cgggcaccgg tggcagcata cagtaagcac tgaactgcgg
    15901 gctgcgtctg ccaggagtgc atttttcccc actccagcgc cgactgaacg atggcctggc
    15961 ggcgcaatga ctgactttcc atgctcacgc gagaagcaaa actgcgatcc tgctgactca
    16021 ttccttgcaa tagcaagcta cccaaaatca gcagcatcag gaccagagcc agtgacgaaa
    16081 cacccttttc gcggttcaca ggttggatcc tgtcacgcta tagctggcat ccaccacggt
    16141 ttgcggatca gccttgctgg cagcgtgcat attaaccgtc aacaccggcg agaagccgct
    16201 gacatcctga cgtacgacct gaaaagtgtc gataatgatg gcatccggat tagtgacttt
    16261 atcccagccc ttaccttcac aggatgtcgc accgcgtagc gtttccagca catgctcctt
    16321 cagacgaaat ccaatctggt cggactcttt taccggttcg cgatcccaga taccattact
    16381 gttcgcatcc cactgcacaa tgacacagtc accctgtccg acaatttcca gcccttcgcc
    16441 cgtacagctg ccatggcaat aacccgccct ctggagatgc ttcgcgacgg taaatacccg
    16501 cagccagatt tcatcttcca gcgccagctt acgggtgttc gttaaacttt cacgctgtaa
    16561 cgcaggcaga aagcgtgcag cccccagcaa caatacgcta ctgatcgcca tagcaatcaa
    16621 cacttccagc agagaaaaac cttgctcttt tacaggcatc cttctgtttc tccttgctga
    16681 caaagccgga gtcttcccca cggcgaaacc accagccacc actcgcccgt tgagtttttg
    16741 aagcgaatat gcccggccca tgcggtattg cgcaggccaa agaaagcaag cgaaggtgtc
    16801 aggtcgctca tttcgacttc gggccagcgc ggcacaaaga ccaatggtga actgccatga
    16861 caggtattcg ccccagcaac ggaactcaca aggcaccata acgtcccctc cctgataaga
    16921 ctgatactgt ggtcgcggtt atgccagttg gcatcttcac gtaaatagag caaatagtcc
    16981 cgcgcctggc tggcggtttg ccaaagccgt tgcgactgct gccagtattg ccagccataa
    17041 agcccacttg cgcttagcat gaccagaatc agcatcgcga ccagcgtttc aatcagcgta
    17101 taaccacgtt gtgttttcat gccggcagta tggagcgagg agaaaaaaag acgagggcca
    17161 gtttctattt cttcggcgca tcttccggac tatttacgcc gttgcaggac gttgcaaaat
    17221 ttcgggaagg tgtctcgaag aatttaacgg agggcaaaaa aaaccgacgc acactggcgt
    17281 cggctctggc aggatgtttc gtaattagat agccaccggc gctttaatgc ccggatgtgg
    17341 atcgtatcct tcaatctcaa agtcttcgaa acggtagtcg aagatggatt cgggtttacg
    17401 tttgataatc aacttcggca gcggacgcgg ttcgcggctt aattgcagat gagtttgatc
    17461 catatggttg ctgtagagat gcgtgtcgcc accggtccag acaaaatcac ccacttccag
    17521 atcgcactgc tgcgccatca tatgcaccag taacgcgtag ctggaaatgt tgaacggcag
    17581 gccgaggaag acgtcacagg agcgctgata aagctggcaa gagagtttgc cgtctgccac
    17641 atagaactgg aagaatgcat ggcacggtgc cagcgccatt ttatccagtt cgcctacgtt
    17701 ccacgctgaa acaataatgc gacgggaatc cgggtcgttt ttcagctggt tcagtacagt
    17761 agtgatctgg tcaatatgac gaccatccgg cgttggccag gcgcgccact gtttaccata
    17821 cactggcccg aggtcgccgt tttcatcggc ccattcgtcc cagatggtga cattgttttc
    17881 gtgtagataa gcaatgttag tgtcgccctg caggaaccac agcagttcgt ggatgatgga
    17941 acgcaggtgg caacgtttag ttgtcaccag cgggaatcca tcctgcaggt taaaacgcat
    18001 ctgatgacca aaaatggaaa gcgttccggt tccggtacgg tcgtttttct gtgtgccttc
    18061 gtcgagcact ttttgcatca gttctaaata ctgtttcatg gttcctcagg aaacgtgttg
    18121 ctgtgggctg cgacgatatg cccagaccat catgatcaca cccgcgacaa tcatcgggat
    18181 ggaaagaatt tgccccatgc tgatgtactg cacccaggca ccagtaaact gcgcgtcggg
    18241 ctggcggaaa aactcaacaa tgatgcgaaa cgcgccgtaa ccaatcagga acaagcctga
    18301 gacagctccc attgggcgcg gtttacgaat atacaggttg aggataataa acagcaccac
    18361 accttccagc agcagctcgt aaagctgtga tgggtggcgc ggcagcacac cgtaagtgtc
    18421 gaaaatggat tgccactgcg ggttggtttg cagcagcaaa atatcttctg tacgggagcc
    18481 agggaacagc atggcaaacg ggaagttcgg gtcaacgcgg ccccacaatt caccgttaat
    18541 aaagttgccc aggcgcccag caccaagacc aaacggaatg agtggggcaa taaaatcaga
    18601 gacctggaag aaggaacgtt tagtacggcg ggcgaagata atcatcacca cgataacgcc
    18661 aatcaggcct ccgtggaagg acatgccgcc gtcccagaca cggaaaagat acagcggatc
    18721 ggccataaac tgcgggaaat tgtagaacag aacataacca atacgtccac cgaggaagac
    18781 gccgaggaag cccgcataga gtaagttttc aacttcattt ttggtccagc cactgcccgg
    18841 acgattcgcc cgtcgtgttg ccagccacat tgcaaaaatg aaacccacca gatacatcag
    18901 gccgtaccag tgaagcgcca cgggtcctat tgagaaaatg accggatcaa actccggaaa
    18961 atgcagatag ctactggtca tctgtcacca caagttcttg ttatttcgct gaaagagaac
    19021 agcgattgaa atgcgcgccg caggtttcag gcgctccaaa ggtgcgaata atagcacaag
    19081 gggacctggc tggttgccgg ataccgttaa aagatatgta tatgatccgc gctataaccc
    19141 cccgcgaatc agcccgccca tgccgcgacg ctccataaag gcagcaacct gatggcgaac
    19201 ttcggtcgcc agttgcgctt ccagactacg ctgcgcaaga ttttctgctt cagcaaaatc
    19261 aatgcgccgc agcaggtatt ttacgcgcgc tacagaacgt ccgttcatag acagatggcg
    19321 atacccaagc ccaatgagga ttgccacgca catgggatcg cccgccattt caccgcacaa
    19381 acggagatcg attccatgta tttccgcttc ccgggcgatc atcgccagag ctcgtaacat
    19441 tgcaggatga agactgtcat aaatgttcgc cacccgggta ttgttgcgat caacggccag
    19501 gatgtattga gtcagatcgt tggtgccaac agagatgaaa tcgacccgct ttgccagatg
    19561 cggcagcata aataccattg acggcacttc cagcatgatg ccgatacgtg gtttgggaat
    19621 ttcgtaaccg atcatctcct cgacttcacg tccggcacgt tcaatcaggc ggcgtgcttc
    19681 gtcaacttca tcgaggcttg tgaccatcgg caacagaata ttcaggttgc ccgtagcggc
    19741 attagcacgc agcatcgccc gcacctggat caagaagatc tccggctgat cgagcgtaat
    19801 gcgaatccca cgccaaccca ggcatggatt ctcttcgcta atcggcatgt aaggcagctg
    19861 cttatctgct ccgacatcca gcgtacgcaa ggtgacgggt ttatcattaa acatttgcag
    19921 catcccctga tactgcgcca cctgttcttc ttccgacgga aaaccacttt gcagcatgaa
    19981 tgggatttca gtgcgataaa gtcctatgcc atcaatacgg ctgcccagtt tttcttcatg
    20041 ttccgggctt aaaccggcat tgagcatgac tttaatgcgt tcgccgcttt ttaactgggc
    20101 gggtaaattg acgtcatctt ccgccagacg gctaagctcg atctcttcac taattagccg
    20161 ctgatattct tgcagcagta ccggttccgg atcgaccagc aattcaccgc gataaccatc
    20221 gacgatcagc gtccgacgat gcagtaccga aggctgaata tccgcgccca tcacggtagg
    20281 tatccccagc gcacgtacca tgatcgcagc atgggagttg gcagcgccat cgcgcacgac
    20341 aacaccgact aagcgatcct ggggcagctc agcaagcgtt gtcgctgaca gttcatctgc
    20401 caccagaatg aaacgttccg gccaggcgtt cggcccttga ttagcgtcat caagatgaaa
    20461 cagcaatcgc tgacccagcg cacgtaaatc gccagcccgc tctttgagat agttatcgct
    20521 tagcgcggca aactgttcgg caaatttttc aatgaccgtt tttaccgccc actctgccac
    20581 cgagccttta tcaacctcgg caaacaattc gcgacgcagc cgggtatccg aaagcaggtg
    20641 tgagtaaaga tcgaaaatag ccgccgtttc tttttgtgca ccggcagcaa agcgtttgct
    20701 atagcggcga aactcgttag ccgcctcttc caacgccccg gtcagtcgtt cgcgttccag
    20761 agccggatcc agcgttgatg cctgatacac ctgttccatt aaaggtaacg tggcatcctg
    20821 ccagccttcg gcaatcgcca caccaggtgc tgccggtaat gcacggattc gcgtctggcg
    20881 atattgccca aacaaggcag tcaactgcga ctgagaaaga atagctgcca tctgggtggc
    20941 aagcgtcacc aggaaggatt cttcactttc gtcatactgg cgcaactctc gttgctgtac
    21001 caccagtaca ccaagcaact ggcgacgttg aataattggt acgcctaaaa acgcgcggaa
    21061 acgttcttct tttacggagg ggatgtattt gaagctgggg tgcttttgcg catctgcaag
    21121 gtttatcggt tccgccagcc tgccaaccag gccgacgatc ccttcatcaa acgcgagcgt
    21181 tacagtgcga ccgcgtggtt ttttcagtcc ccgggtcgcc atcaggtagt aacaacgtcg
    21241 atcatgatcg gccaggtaga ccgaacagac ctcggtatcc atcgcaagac agatgtcggt
    21301 aaccagaata tttaacgcct cattcaggcg tggtgcgctg gctacctttt cgactatttc
    21361 gcgcaggcga gtgagcataa ttggcgtgac ttaacctctt ttacgtcgat aagcagatgc
    21421 gttttgtggt tttggcgtat tttcctgcag tgacatcacc acactcgcga actctttcat
    21481 taccctacgg tagacatcac gtttaaatga caccacctgt ctgactggat accagtaact
    21541 tacccatcgc cagccgtcaa actctggtgt actgctggtt tgcatattga tttctgcatc
    21601 gccgctcacc agctgcaaga gaaaccattt ttgtttttgg ccgatacaaa ccggcttcgt
    21661 gtcccaacgc accaaacgtt tcggtaattt gtagcgcaac cagttacgcg ttgaagcaag
    21721 gattcggacg tctttgcggc ttaatcctac ttcttcaaac aattcacggt acatcgcctg
    21781 ctctgcggat tctccggggt tgattccgcc ttgcggaaat tgccaggagt gctgaccaaa
    21841 tcgccgggcc cacattacct gcccctggcg attacaaatc acgataccta cgtttgggcg
    21901 gtagccatcg tcatcaatca ccggactacc tcaaaataaa gctttatata cgaatgattg
    21961 tttcatactc caggaagacg gtaaaccact ctctgcaggg cattacacac taataacaat
    22021 tgaataactc acagttatgt gcagagttat aaacagagga aggggtggat agccgttttt
    22081 attcaccttt tctgtggata gagttgtgaa gaactacgga attactacgg gaaaacccgg
    22141 agcattctga ataagacaga tatagagatt ttaaaatatt cattattagt caatgcatta
    22201 catgtgattt gagtaataaa aaagagtaat tcgtgaccca ggtcacacct ctcatttacg
    22261 tgttggcgaa agatcaacca atgccgtatt tatccacaga atgtgccact aagttaagca
    22321 ctgaaccact aaaaactgga gtttcgtcgc acgtcaaggc tgtaaatgga aacagtagtg
    22381 gaggtttttc acagttatcc cagctttctg tggataacat ggtgtaagat cctgtttatt
    22441 ttcagtgacc agatttggaa aacccgttgc agtgttgcgc aactcgttta ccggcaacct
    22501 aaaaagcaat ataaatcaga gaattgaaca acgcatgtgg aaaaagttac actgcgaata
    22561 ttcagcacat aattgctgtt tgttttttaa ccaaggtatc atgacatgtc ccaacctcgc
    22621 ccactgctct ctcctcccga aactgaagaa caattgttag cgcaagcaca acaactttct
    22681 ggttatacat tgggagaact ggcggcactt gccgggctgg ttacgccgga gaatttaaaa
    22741 cgcgataagg gctggattgg cgtgttactg gagatctggc taggtgccag cgcagggagt
    22801 aaacctgagc aagattttgc tgctctgggc gtggaactta aaactatccc tgtggatagt
    22861 cttggtcgtc cactggaaac aacattcgtt tgtgttgccc cgttaacggg caatagcggg
    22921 gtgacctggg aaaccagcca cgtgcgccac aagctcaaac gcgtgctgtg gataccggtt
    22981 gaaggcgagc gcagcatccc gctggcaaag cgtcgcgtag gatcaccgtt gctgtggagc
    23041 ccgaatgaag aggaagaccg gcagctacgc gaagactggg aagaattaat ggatatgatt
    23101 gttctcggtc agattgagcg gatcaccgct cgtcacgggg aatatttaca gatacgaccg
    23161 aaagcagcga atgcgaaagc gctgaccgaa gctattggtg cccggggcga acggattctg
    23221 acgctgccgc gcggatttta tttgaagaag aatttcacca gtgcgctact ggcccgtcat
    23281 tttctgatcc agtagccatc gctttgacct gctgctttcc gggcatataa ttaccgcttc
    23341 atttttttgg cagggctttt tagatgttat ttgcatggat aaccgatcct aacgcctggc
    23401 ttgcgctcgg tacactgacg ctgctggaga tcgttcttgg gatcgacaat attattttcc
    23461 tttctctggt ggtggcaaag cttcccacag cacaacgtgc tcatgcgcgc cgtctggggt
    23521 tggcgggagc catggttatg cgtctggcgc tgctggcatc catcgcctgg gttacgcgcc
    23581 tgacgaatcc gctttttaca atattcagtc aggaaatttc cgcccgtgat ttgatcctgc
    23641 ttctgggtgg cttgttcctt atctggaaag ccagcaagga aatccacgaa tccatcgaag
    23701 gtgaagaaga agggctgaaa acacgcgttt catcattcct cggcgcgatc gtgcagatta
    23761 tgctgctgga tattatcttc agcctcgact cggtgattac cgctgtgggt ctgtcagatc
    23821 acctgtttat tatgatggct gccgtggtga ttgccgtagg cgtgatgatg ttcgccgcgc
    23881 gctcgattgg tgattttgtc gagcgccatc cttcggtaaa aatgctggcg ctctctttcc
    23941 tgattctggt gggctttacc ctgattctgg aaagtttcga catccacgta ccgaaaggtt
    24001 acatctactt cgcgatgttc ttctctattg cggttgaaag cctcaacctt attcgcaaca
    24061 aaaagaatcc gctctgatac tccgtacgct ctcctgcggg agggcgtact cactcctgtt
    24121 accctcacag tttaagattt tcctgctttc aagatatata acgtcggttt ataaacagac
    24181 tattatcata ggtgggccaa atgaggataa ccggatgaaa aaatgggcag taataatttc
    24241 tgcagtcgga ctggcgtttg ctgtttccgg gtgttccagt gattacgtca tggcgaccaa
    24301 agatggccgt atgattttga ccgatggaaa acctgaaatt gatgatgata ccgggctggt
    24361 gagttatcac gatcagcaag gtaacgcgat gcaaattaac cgtgatgatg tttcgcaaat
    24421 tattgaacgt taacaaataa ggtcagcatc cggctggcct taagattttt ctctttccct
    24481 tttcccttcc ctctgccatt tttatattcc ttatgtcgtg attataaaaa ggaaacggct
    24541 atgcaatatc accgtatacc ccacagttcg ctggaagtca gcacgctggg gcttggcacg
    24601 atgacgtttg gtgaacagaa cagcgaagcc gacgcccacg cacaactcga ctatgccgtc
    24661 gctcagggca ttaaccttat cgacgttgcc gaaatgtacc cagtacctcc gcgccccgaa
    24721 actcaagggc taaccgaaac ctacgtcggc aactggctgg cgaaacatgg cagccgcgaa
    24781 aagttaatta tcgcctccaa agtgagcgga ccgtcgcgca ataatgacaa aggcatccgc
    24841 ccggatcagg cgctggatcg gaagaatatc cgcgaagcgc tgcatgacag cctcaagcgt
    24901 ctgcagactg attacctcga tctctatcag gtgcactggc cgcagcgccc aaccaactgc
    24961 ttcggcaaac tcggttatag ctggacagat tctgcgcctg cggtttcgct gctggatacg
    25021 ctggacgcac tggcagagta ccaacgcgcg ggaaaaattc gttatatcgg cgtgtcgaac
    25081 gaaactgcat ttggcgtaat gcgctacctg catctggcgg acaaacacga tctgccgcgt
    25141 attgtcacca ttcagaaccc ctacagcctg ttaaaccgca gttttgaagt aggtctggca
    25201 gaagtcagcc agtatgaagg ggtcgaactg ctggcctatt cgtgcctggg tttcggcaca
    25261 ctgaccggga aatatctcaa tggtgcaaaa cccgctggcg cacgtaatac gctctttagt
    25321 cggttcaccc gctatagcgg tgagcaaacg caaaaagccg tcgcggcgta tgttgatatc
    25381 gccagacgtc atggcctgga ccctgctcag atggcgctcg catttgtacg ccgtcaaccg
    25441 tttgttgcca gcactctgct gggcgcaacc acgatggagc agctgaaaac taacgtcgaa
    25501 agtttgcatc tggagttgag cgaagacgta ttagctgaaa ttgaagcggt gcatcaggtt
    25561 tatacgtatc ctgcaccata agattaacgc cggttaaaac cggcgttaaa tattaatgac
    25621 ggcgctgcca gatccacagc gccgttattg ccagcgcaaa cagcgcaccg aagccaatgc
    25681 caatgggcac gaccgggatg cctaccatta ccgccagcga gtaaatgccc agcatcaaca
    25741 acatggcgct gttttcgcca aggttttgta ctgcaatcgc attccccgcc ccgacgcttt
    25801 ttttaccccg ctcctgtagc aacgcattga gcggaacgac aaaaaagccc cccagcacgc
    25861 caatcagcat caacaaagca taggctggca gcagctcgtg ttgcagggag aaaatcagta
    25921 ccaccacgcc aatcaaaatc ccggctggca tacagcgtga cacggtttcc agcgtaacta
    25981 acttcgctgc cgcacctgca ccaaccacga taccaatcgc taccatcgcg ttgagatagg
    26041 tcggcgtagc gttatcggta atacccagcg ccaccggtac ccacagcacc aacaggaaac
    26101 gcagcgtgac acccgctccc cagaataaac tggtgcccac cagcgaaaaa cgcgtttcac
    26161 cattgcgcca tagcgaggtg caggcattca ggaaactgcg ggtcatgctg atgagattcc
    26221 aggactgccc cggacgcgcc gccgccagtt tgggaatgta gatattggca acgaccgcgc
    26281 caccgtaggc cagtgcgcat acggccaggg caaccagaat atgccagtca gccagaacac
    26341 caccagctac ggaaccgagc aaaatcgccg ctatggtaga cgcttccatt aaaccgttag
    26401 cttttactaa cttactaccc gtggttaatt cgccgagaat accgtatttc gccggtgaat
    26461 aggctgcagc accaacaccc accaacgtat agccgagaaa cggattgata ccaaagcaga
    26521 tactggctgc gcccagtagc ttcaggccgt tggcaaacat catcacccgg cctttggcga
    26581 agctatccgc cacctgcccg acaaacggcg caaaaagaat gtaagcacct acaaacacca
    26641 tttgcaggat gggctggctc cactccggat agaactgcgc tttcagtaac gccagagtgg
    26701 caaacagtag cgcattatcg ccaaacgcag agagaaactg cgccacgata accgctttca
    26761 tccccttcga ccacaacgaa gtgttagtgt gcactgactc actcatcgtg ttgttccgct
    26821 tcgtctaccc agcttttcaa agtgacaaag tcaggtttgc cgctgccaag taatggcatc
    26881 tgtttcagat agcgaatatc gcgcggtaca gcaagctccg gcacgccgtg ctcgcgggca
    26941 tactgttgca acttatcgcg cgtcagttcg ttatctgtgg tgaaaagcac cagtgcctcg
    27001 cctttgctgg catcgctctt aatcgcagtg gcatggactt tatctggcga aacaccaagt
    27061 gccagttgtt ccaccatttc cagcgacacc atttcgcctg caattttggc aaagcgtttt
    27121 gcgcggccct gaatctgcac aaagccctgc tcatcaaaac gcacgatatc gccagtgtca
    27181 taccagccgc gctccatttc gccgcgaaca ttctcggcgg tgggcacttc cagtacacca
    27241 ggcttctcca cccgcagata gccgttcatt atgttcggcc ctttcagttg caggcgtccg
    27301 ccctcttcga tacctggaac tgacaacagg cgcgcatcca ttccaggtag aatacgccct
    27361 accgtaccgg gtttcgccgc catcggtacg ttgatagaaa cgacaggcgc gcattcggtc
    27421 acaccgtagc cttcaaggat gcgcaggcca aatttatcct gccaaagctg tttagtactt
    27481 tcttgtagtt tttctgcgcc tgccaccaca tagcgtagac gatagaagtc atacgggttg
    27541 gcaaagcgcg cgtagtgacc gaggaaagtc gaggtaccga acaacacggt gcaactgcgg
    27601 tcatacacca actccggcac aatgcggtaa tgcagcgggc ttggataaag gaacacttct
    27661 gcacctgtga gcagtggcgt aaacaggcct accgtcagcc caaaggagtg aaacagcggt
    27721 aacgccgaca taaagcgatc gttggtggtg aagtcggcaa tcgttttaat ctgctcgaca
    27781 ttcgccagaa tgcttttatg gctatggacg acgcctttcg gatggccttc agaaccggag
    27841 gtaaaaagga tcagcgcctc ttcttccggc tgctgtttaa cctgtgccag acgcggcatc
    27901 agcaaatgag cgaagatcca tactttgtcg gcagtggtga catctgcttt taaatcttcc
    27961 agatagaccc agcgcacctg agtaagttgc tccggcagat gccagagttt gcctttatcg
    28021 agaaactgac gggaagtgaa aatggttttg atttcagccg ccgtaatagc actggtcaac
    28081 ccttttaccc cggcggtgta gttcatcatt gcggggatgc ggcgacgggc gatggcccca
    28141 aaaatcactg cagcactgat gcctgcattg ggcagcatta agccgatgcg ttcgccttca
    28201 acactgtatt tttcaaggat gcgtccaaca aacagcgttt tcgtaagcaa tttgcgatag
    28261 gagtccgggg taaagttaac gtcttcgaca catttcttcc cggctccgaa gcggtacatt
    28321 gcgctcagta aagattcgta cagcgtttca cgcgggcgca ccgccatacg cgcttccatc
    28381 attatttgat gcagcatttc gccagcgatt ttgcgacggt cacgggcacg cggcgcatcc
    28441 ggcatctcca cctgcgttgg tggcaaaata tgcagagtaa tttgcgggaa caagcgacgt
    28501 ttaaccagac ctttcaggcg gctgaagtgc gtaagttccg ccccttcaat acgcacagga
    28561 ataaccgttg caccagactt cgccgcgaca aatcccgcgc catcgtagat tttcatcagc
    28621 gagccagtgg tggtgatgcg tccttcaggg aaaatcacca ctggtcgtcc ctgttccacc
    28681 agacgtacca gatgtttaat cgccataggt tgcgtcgggt cgagaggaac aaagtcgata
    28741 aatgatttca gccaacgcat ataccactgt tggcttattg aggtgtaaac ggcaaatacc
    28801 ggacgtacgg gtaaaaacaa tgccagcagt atgccatcaa taaaagagac gtgattaggc
    28861 gtaattagaa cgcgctcgcc cttcagtgcc ttggtgtcac ccgtaacgcg aacgcgatac
    28921 aaaacacggc acaaatttcg aaaaaagcta aaaagcattc caactccctt tgctctgatt
    28981 cagtaaaagc gaatggaggg agattacacg agataaagaa cgcgagcgac agtaaattag
    29041 gtgcgaaaaa aaacctgcgc atccgcgcag gttggtgcaa gagactgggt acgaagagcg
    29101 taccgaataa tctcaccaat caatacctct gggatcttga ttgtggtctg cacaacgact
    29161 cttcgccagc gagaaaacgc aaaggaatga agggaaatgc aacgaggtgt gtaaattgtc
    29221 ggttactgtt acagattgat gaccggcaaa aaaaaacctg cgcatctgcg caggctggtg
    29281 taattcatgt gctcaacccg aagttgactt cacctatcaa tacctctggg atcaccactt
    29341 tagcaacctg aagccaaacg ccaccagcgg tcaatcgcaa cagcgtttcg caaagtgtaa
    29401 ccaaaggttt gcaatctctt tttttgtctt acttatttgc gtttggctca cattcccaca
    29461 atgaaaacac gccgcccctt gaaccaacgg gcgttttccg taacactgaa agaatgtaag
    29521 cgtttaccca ctaaggtatt ttcatggcga ccataaagga tgtagcccga ctggcaggcg
    29581 tttcagtcgc caccgtttcc cgcgtcatta ataattcacc caaagccagc gaagcttccc
    29641 ggcttgctgt gcatagtgca atggagtctc ttagctatca cccgaacgcc aacgcccgtg
    29701 cgctggcgca gcagaccact gaaacgatcg gtctggtcgt tggtgatgtt tccgatccgt
    29761 ttttcggcgc aatggtgaaa gcggtcgaac aggtggctta tcacactggt aattttttat
    29821 tgattggcaa cggttaccac aacgaacaaa aagagcgtca ggccattgag caactgatcc
    29881 gccatcgctg tgctgcgctg gttgtccatg ccaaaatgat cccggatgcc gatttagcct
    29941 cattaatgaa acaaatgccc ggtatggtgc tgatcaaccg tatcctgcct ggctttgaaa
    30001 accgttgtat tgctctggac gatcgttacg gtgcctggct ggcaacgcgt catttaattc
    30061 agcaaggtca tacccgcatt ggttatctgt gctctaacca ctctatttct gacgccgaag
    30121 atcgtctgca agggtattac gatgcccttg ctgaaagtgg tattccggcc aatgaccggc
    30181 tggtgacatt tggcgaacca gacgaaagcg gcggcgaaca ggcaatgacc gagcttttgg
    30241 gacgaggcag aaatttcact gcggtagcct gttataacga ttcaatggcg gcgggcgcga
    30301 tgggcgtgct caatgataat ggtattgatg taccgggtga gatttcgtta attggctttg
    30361 atgatgtgct ggtgtcacgc tatgtgcgtc cgcgcctgac caccgtgcgt tacccaatcg
    30421 tgacgatggc gacgcaggct gccgaactgg ctttggcgct ggcggataat cgccctctcc
    30481 cggaaatcac taatgtcttt agtccgacgc tggtacgtcg ccattcagtg tcaactccgt
    30541 cgctggaggc aagtcatcat gcaaccagcg actaaccgca gttaaagcaa ttccagcgcc
    30601 agtaattctt caatggtctg gcgacggcga atcaaccgcg cctgaccatt atcaaacaga
    30661 acttctggta acagcggacg gctgttgtag ttggatgaca ttgatgcgcc atatgcccct
    30721 gtatcatgca gtaccagata atcacccgcc ttcacttccg gcaaggcgcg ggtttcgaca
    30781 tttccccctt cctgctgggt aaagacatcg cccgattcac ataacggccc ggcgacgacg
    30841 gtttccaccg ttggcgcgtg ttccagagaa cgaccctcag ctgccagggc actgatatgg
    30901 tggtaactac cgtacattgc cgggcgcatc agatcgttga acccggcatc aaccagcaca
    30961 aagtggcggc tacccatttg tttgacgctc cgcacttgag taattaatac gccagcctgc
    31021 gctaccagaa agcgacccgg ttcaatttcc agtttcacag gatggcccaa atggcgggcg
    31081 atttgctcac gcgcggcatt ccacagacca taataatgtt cggtatcaac cgcctcttca
    31141 ccctgttgat aaggaataga aagcccaccg cccgcagaaa tagcctgtaa atcctgaccg
    31201 aactcgagga cctgacgcac catagcacca catacctgtt ccagatgggc ataatcgacg
    31261 ccagaaccaa tgtgcatgtg aatgccgacc agctgtagat gatgacgttg tatcacgtcc
    31321 agtgcggcgg gcagatcggt gtaccagata ccgtgcttgc tgttttcgcc accggtattg
    31381 gttttttggc tatgcccgtg accaaacccc ggattaacac gcagccatac ccgatgccct
    31441 ggagaaacct gacccagttg gtcgagcata tcaacagaac ccgcattcac cggaatttgc
    31501 aattcactga cgcgttcaag cgtcgcctga tcgataacat ctgccgtaaa aaccatatca
    31561 tcggggtgcg tttgcggatt gtaacccgcc gccagtgcac gctctatttc gcctaacgag
    31621 acggaatcca cttttacgcc ctgctcacgc attaagcgca aaatatgaat attggaacag
    31681 gctttctgtg caaagcgcac cacatcaaac tgtttcagcg ctgcaatctg ccgacgaata
    31741 atttgcgcat cgtagaccca caccgggcag ccaaattctg cgggcaaacg cagcagattt
    31801 tcggcggtga gatcggtatc agtgctgaac agtgaatgtg gcataacaaa ctccagataa
    31861 gtgctttttt atgattacgc cacatcataa aaagaataaa aaatatcgat ttatgtcgag
    31921 tctatgcaaa aatgatatgg attaccggat tgcgagagag cgctaatggc cgcagttaac
    31981 ttacgtcata ttgaaatttt tcatgcggta atgaccgccg gaagcctgac tgaggcggca
    32041 cacctgctac acacctcaca gccaaccgtc agccgcgaac tggcgcgctt tgagaaggtg
    32101 atcgggctga aattgtttga gcgcatacgt ggacgattac atcctaccgt gcaaggactg
    32161 cgtctgtttg aagaagtgca acgatcctgg tacggactgg atcgcattgt cagtgccgca
    32221 gaaagtctgc gcgagtttcg ccagggagaa ctgtctattg cctgcctgcc ggtcttttcg
    32281 caatcttttt taccgcggct cctgcaaccc tttctggcac gttatcccga tgtcagctta
    32341 aatatcgtgc cccaggaatc accgctactt gaagagtggc tctcggccca gcgtcatgat
    32401 ttaggactca ctgaaacgct ccatacgcct gcgggaacag aacgtaccga attactctct
    32461 ttagatgaag tgtgtgtgtt acctccgggc catccgctgg cggtaaaaaa ggtattaacg
    32521 ccggatgatt ttcacagtga gaactacatc agcctttccc gtactgacag ctatcgccag
    32581 ttgctggatc aattgtttac tgagaatcag gttaaacgac gcatgatcgt agaaacccac
    32641 agcgccgcgt cagtctgcgc aatggtacgg gcgggggtag gcgtttcggt ggttaacccg
    32701 ctcaccgcac tggattatgc ggcaagcggt ttagtggtgc ggcggttcag cattgcggtt
    32761 ccattcaccg tcagcctgat ccgccccctg caccgcccgt catcagcgct ggtgcaggcg
    32821 tttagtgagc atttacaagc gggattaccg aaactggtca cttctcttga cgctattttg
    32881 tcgtcagcta cgacagcata aaagcgacag catcctcggc atggatcgcc gcggtatcaa
    32941 acacaggcag aacactgcgc tcttctggca ccagtaaacc aatttctgtg cagccaaaaa
    33001 tgacgccctg tgcgccctgt tctgcaaggc gagcaatcac ttgcgcataa taagcgcgtg
    33061 acgcttcggt aaattgcccc agacacagtt cttcaaaaat aatctgatta attttcgccc
    33121 gttcatccgc ttcaggaata agacagttga tggaaaattg ttccgtcagc cgcccgcgat
    33181 aaaaatcctg ttccatggtg taacgtgtac ccagcagcgc cacacgagtc attcctgccc
    33241 cggtaattgc acgtccagtg gcatccgcta tgtgtaagaa aggcagagag caacgagact
    33301 caatggcatc cgccacttta tgcatcgtat tggtgcataa cacaatacct tctgcgcccg
    33361 cccgctgtaa gccaagcgcc gcctcagcca gaatatcccc ggttttgtcc cactcaccgc
    33421 gacgctggca ctcttctatt tcatgaaaat cgacgctatg tagcagcact tgcgcagagt
    33481 gaagcccacc aagccgctgt ttaatgcctt catttatcag acgatagtaa ggaatagtgg
    33541 attcccagct cattcctccc agcaaaccaa ttgttttcat tcctctctcc tgatgtgtgt
    33601 taccgcaaca aggctgaata aggaacgaga caaacgcctc aacggccagg tgcccaatct
    33661 ctattaacga aaaaagggcc ggatgtacag cacatccggc ccgtgaaatc agacgccgat
    33721 atttctcaac ttttcgcctg ccatcagttt gcgctcgata tgttccagcg tgacattttt
    33781 ggtttccgga atgagccaga aagtgatgcc cacaaacgca atgttcagcg cagtgtagag
    33841 ccagaacgta ccggcagcgc caatgctatc aagcagtgtc aggaaggtcg cgccgataat
    33901 catattcgac acccagtttg tcgtcgttga gcaggtgatg ccaaaatcgc ggcatttcag
    33961 cggctgaatt tcagagcaca agatccacac cactggcgcg gcgctcatcg cataaccggc
    34021 aatacacatc atcgtcatgc caacagagag ccaggacaag ccactggaag ccgtaccgtt
    34081 atcaaactgc atcaggcagt aacccagcac cagagtgcct aacgccatca cgctgaaacc
    34141 aattttcaga gccggcttac gccctgcttt atctaccgta aagaccgcaa tgaaggtagc
    34201 gaacataaag gtcagcccca cgaccagagt cgcaatcatc tgttgttctg tggtcgtaaa
    34261 gcccgccatt ttgaagatac gtggcgcgta gtacatgatg atgttcatac cggtaaactg
    34321 ctgcatcgcc tgcaacaaca taccgagaaa tacagcacga cggacgttac ggttgatctt
    34381 aaacaatgcc caaccgccct gttttaactt caggctttca cgaatttcgt tgagttcttc
    34441 tcgcgctttt tccgacgtat cgcgcagcat acgcaatact tcttccgcct caatatgacg
    34501 ccccttttcc gccagccagc gcgggctatt tggcaggaag accaccagaa taatcagcag
    34561 aactgctggt aaagcaagaa cccccaacat tgcgcgccag ttaccgctat aactgaacgc
    34621 tgtatcggat aaaaacgcca gcacgatgcc gagagtgacc atcaactggt acatgctgat
    34681 catcttaccg cgaacgtttt cacttgccat ttcagaaaga tatagaggag cggtgtaaga
    34741 cgcgatcccg acagcaatgc ccaacaccac acgagcggcg attaacatct ctacgctggt
    34801 cgcaaaagcg gaccctatcg aaccgagtac aaacaggatg gcccccgcca tcaggctgta
    34861 tttacgcccc aggcggaacg acagccaacc attaaacagc gcaccaattg ctgcgccgag
    34921 catcatgcta ctgaccaccc attcctgcaa acgactggtc agcacaaagt gatcggtaat
    34981 gaacggcaac gctccggcga ttacgccgat atcaagacca aataacaatc ctgcgaccgc
    35041 agcagctacc gaaacaaaca tattcatacg ccgcgtatcc cgcagaggac gtggcgttaa
    35101 agcagattcc gtattgatag taaccatttt ttcctgccag cagagagtaa gacatagtga
    35161 aaaaatacgt gaacaattca cacaggtgtc aggtcggaaa tagcataaat atggattaaa
    35221 ttgctacgac atgtcgttat gtgatggata ttccaatttt caaattaagt tgaattattg
    35281 agattattat taaccaccta attttaaagc agataaaatt cataaagttc attaattgat
    35341 aattgatatg gattatttca taaccatgat atggattatg atgatctaca gatataaaaa
    35401 accctgccat gcggcagggt
//
LOCUS       DAZFFE010000036        35360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFFE010000036 DAZFFE010000000
VERSION     DAZFFE010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.036"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            274..1659
                     /gene="dcuC"
                     /locus_tag="ACHJ69_002003"
     CDS             274..1659
                     /gene="dcuC"
                     /locus_tag="ACHJ69_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO5014992.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1700..2380)
                     /gene="dpiA"
                     /locus_tag="ACHJ69_002004"
                     /gene_synonym="citB"
     CDS             complement(1700..2380)
                     /gene="dpiA"
                     /locus_tag="ACHJ69_002004"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO5014993.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2349..4007)
                     /gene="dpiB"
                     /locus_tag="ACHJ69_002005"
     CDS             complement(2349..4007)
                     /gene="dpiB"
                     /locus_tag="ACHJ69_002005"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO5014994.1"
                     /translation="MLQLKENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            4387..5445
                     /gene="citC"
                     /locus_tag="ACHJ69_002006"
     CDS             4387..5445
                     /gene="citC"
                     /locus_tag="ACHJ69_002006"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO5014995.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            5460..5756
                     /gene="citD"
                     /locus_tag="ACHJ69_002007"
     CDS             5460..5756
                     /gene="citD"
                     /locus_tag="ACHJ69_002007"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO5014996.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILEVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5753..6661
                     /gene="citE"
                     /locus_tag="ACHJ69_002008"
     CDS             5753..6661
                     /gene="citE"
                     /locus_tag="ACHJ69_002008"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO5014997.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHTLQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            6672..8204
                     /gene="citF"
                     /locus_tag="ACHJ69_002009"
     CDS             6672..8204
                     /gene="citF"
                     /locus_tag="ACHJ69_002009"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO5014998.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYSGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGAAVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            8208..8759
                     /gene="citX"
                     /locus_tag="ACHJ69_002010"
     CDS             8208..8759
                     /gene="citX"
                     /locus_tag="ACHJ69_002010"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO5014999.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8734..9612
                     /gene="citG"
                     /locus_tag="ACHJ69_002011"
     CDS             8734..9612
                     /gene="citG"
                     /locus_tag="ACHJ69_002011"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO5015000.1"
                     /translation="MSMPATSTKNTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQSVTPITICATAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            9663..11126
                     /gene="citT"
                     /locus_tag="ACHJ69_002012"
     CDS             9663..11126
                     /gene="citT"
                     /locus_tag="ACHJ69_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO5015001.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            11240..12046
                     /gene="rna"
                     /locus_tag="ACHJ69_002013"
     CDS             11240..12046
                     /gene="rna"
                     /locus_tag="ACHJ69_002013"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO5015002.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGNTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            12276..12686
                     /gene="rnk"
                     /locus_tag="ACHJ69_002014"
     CDS             12276..12686
                     /gene="rnk"
                     /locus_tag="ACHJ69_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO5015003.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEIPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            12829..12918
                     /gene="yldA"
                     /locus_tag="ACHJ69_002015"
     CDS             12829..12918
                     /gene="yldA"
                     /locus_tag="ACHJ69_002015"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO5015004.1"
                     /translation="MSEALGITAFYIFIAAIIVAAVLYLEQRW"
     gene            13042..13470
                     /gene="uspG"
                     /locus_tag="ACHJ69_002016"
     CDS             13042..13470
                     /gene="uspG"
                     /locus_tag="ACHJ69_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO5015005.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(13592..15157)
                     /gene="ahpF"
                     /locus_tag="ACHJ69_002017"
     CDS             complement(13592..15157)
                     /gene="ahpF"
                     /locus_tag="ACHJ69_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO5015006.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(15328..15891)
                     /gene="ahpC"
                     /locus_tag="ACHJ69_002018"
     CDS             complement(15328..15891)
                     /gene="ahpC"
                     /locus_tag="ACHJ69_002018"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO5015007.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            16263..17009
                     /gene="dsbG"
                     /locus_tag="ACHJ69_002019"
     CDS             16263..17009
                     /gene="dsbG"
                     /locus_tag="ACHJ69_002019"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO5015008.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLSVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            17214..18116
                     /gene="ybdO"
                     /locus_tag="ACHJ69_002020"
     CDS             17214..18116
                     /gene="ybdO"
                     /locus_tag="ACHJ69_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5015009.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     YGPQLISCTNNNILIRCLRQDTSIDIECHDILLSAENAEELLVQRKTDLAITLQPVIS
                     RSVICMPLHTIRNSLICSNKHPRITDTSNYEQIIAEEFTLLISKSAGIDEIQMDIDER
                     FMNRKVSFRSSSLMTIINSISVTDLLGIVPIELYDLHRDFLKLKEIKLEQPLPAVKLY
                     ISYNKASLNNLVFSRFIDRLNDSF"
     gene            18263..19483
                     /gene="ybdN"
                     /locus_tag="ACHJ69_002021"
     CDS             18263..19483
                     /gene="ybdN"
                     /locus_tag="ACHJ69_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO5015010.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRICVSFSGGKDSGLML
                     HLTAEIARQMGKKICVLFIDWEAQFSCTINYVQSLREFYADVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQQLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            19456..20085
                     /gene="ybdM"
                     /locus_tag="ACHJ69_002022"
     CDS             19456..20085
                     /gene="ybdM"
                     /locus_tag="ACHJ69_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO5015011.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQFSRAWTVK"
     gene            complement(20086..21246)
                     /gene="ybdL"
                     /locus_tag="ACHJ69_002023"
     CDS             complement(20086..21246)
                     /gene="ybdL"
                     /locus_tag="ACHJ69_002023"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO5015012.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTRMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDVDSNITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQTDFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     IADMLHAEPEHYLALPEFYRQKRDILVNALSESRLEILACEGTYFLLVDYSAVSTLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            21355..22443
                     /gene="hcxA"
                     /locus_tag="ACHJ69_002024"
     CDS             21355..22443
                     /gene="hcxA"
                     /locus_tag="ACHJ69_002024"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO5015013.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGVKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFLVLVEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQVEIDKMIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(22453..22650)
                     /gene="ybdD"
                     /locus_tag="ACHJ69_002025"
     CDS             complement(22453..22650)
                     /gene="ybdD"
                     /locus_tag="ACHJ69_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO5015014.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(22763..24868)
                     /gene="cstA"
                     /locus_tag="ACHJ69_002026"
     CDS             complement(22763..24868)
                     /gene="cstA"
                     /locus_tag="ACHJ69_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO5015015.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWQLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(25049..25462)
                     /gene="entH"
                     /locus_tag="ACHJ69_002027"
     CDS             complement(25049..25462)
                     /gene="entH"
                     /locus_tag="ACHJ69_002027"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO5015016.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(25465..26211)
                     /gene="entA"
                     /locus_tag="ACHJ69_002028"
     CDS             complement(25465..26211)
                     /gene="entA"
                     /locus_tag="ACHJ69_002028"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO5015017.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRHQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(26211..27068)
                     /gene="entB"
                     /locus_tag="ACHJ69_002029"
     CDS             complement(26211..27068)
                     /gene="entB"
                     /locus_tag="ACHJ69_002029"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO5015018.1"
                     /translation="MAIPKLQAYALPEPHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAEL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(27082..28692)
                     /gene="entE"
                     /locus_tag="ACHJ69_002030"
     CDS             complement(27082..28692)
                     /gene="entE"
                     /locus_tag="ACHJ69_002030"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO5015019.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GEWQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRTSA"
     gene            complement(28702..29877)
                     /gene="entC"
                     /locus_tag="ACHJ69_002031"
     CDS             complement(28702..29877)
                     /gene="entC"
                     /locus_tag="ACHJ69_002031"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO5015020.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHLAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            30066..31022
                     /gene="fepB"
                     /locus_tag="ACHJ69_002032"
     CDS             30066..31022
                     /gene="fepB"
                     /locus_tag="ACHJ69_002032"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO5015021.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTALVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQIYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(31026..32276)
                     /gene="entS"
                     /locus_tag="ACHJ69_002033"
     CDS             complement(31026..32276)
                     /gene="entS"
                     /locus_tag="ACHJ69_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO5015022.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMIGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSVRPGLLMLLSTLGAFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDN"
     gene            32387..33391
                     /gene="fepD"
                     /locus_tag="ACHJ69_002034"
     CDS             32387..33391
                     /gene="fepD"
                     /locus_tag="ACHJ69_002034"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO5015023.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLMMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLTAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLSTPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            33388..34380
                     /gene="fepG"
                     /locus_tag="ACHJ69_002035"
     CDS             33388..34380
                     /gene="fepG"
                     /locus_tag="ACHJ69_002035"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO5015024.1"
                     /translation="MIYVSRRLIITCLLLLIACVMAGVWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVASHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            34377..35192
                     /gene="fepC"
                     /locus_tag="ACHJ69_002036"
     CDS             34377..35192
                     /gene="fepC"
                     /locus_tag="ACHJ69_002036"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO5015025.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEDAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTTPSTAKI"
     gene            complement(35189..>35360)
                     /locus_tag="ACHJ69_002037"
     CDS             complement(35189..>35360)
                     /locus_tag="ACHJ69_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415119.1"
                     /note="in Salmonella is responsible for long O-antigen
                     polymerization; may be part of the ferric enterobactin
                     transport system; necessary for enetrobactin uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HFO5015026.1"
                     /translation="KYQLSPSLPVKKDGPGKAIIVILSALIGGMVACGGVLLRHAMAS
                     RKQDAIMADHLV"
BASE COUNT         8495 a         9602 c         8826 g         8437 t
ORIGIN      
        1 agattatttt ttgagcacac aaccgcagct aacaccttac aattaacaaa tttgcatcaa
       61 tccaccatca atttacacac attattatgt gatatttgcc aaccgctaaa tatgcgtttt
      121 gttatctacg tataaaaaca gcaacttcaa tgtcttaatg gcagtttttc ttgattttaa
      181 tcagcattca ccgccaattt attaggcata ttttttcctt aacttttagg aattttttat
      241 tatttacttt ggggcctgga gacaggaaaa attatgctga cattcattga actccttatt
      301 ggggttgtgg ttattgtggg tgtagctcgc tacatcatta aagggtattc tgccactggc
      361 gtgttatttg tcggtggcct gttattgctg attatcagtg ccattatggg gcacaaagtg
      421 ttaccgtcca gccaggcttc aacaggctac agcgccacgg atatcgttga atacgttaaa
      481 atattgctaa tgagccgcgg cggcgacctc ggcatgatga ttatgatgct gtgtggcttt
      541 gccgcttaca tgacccatat cggcgcgaat gatatggtgg tcaagctggc gtcaaaacca
      601 ttgcagtata ttaactcccc ttaccttctg atgattgccg cctattttgt tgcctgtctg
      661 atgtcactgg ctgtctcttc cgcaaccggt ctgggtgttt tgctgatggc aaccctgttt
      721 ccggtgatgg taaacgttgg tatcagtcgt ggcgcagctg ctgccatttg tgcctccccg
      781 gcggcgatta ttctcgcacc gacttcaggg gatgtggtgc tggcggcgca ggcttccgaa
      841 atgtcgctga ttgacttcgc cttcaaaaca acgctgccta tctcaattgc tgcaattatc
      901 ggcatggcga tcgcccactt cttctggcaa cgttatctgg ataaaaaaga gcacatctct
      961 catgaaatgt tagatgtcag tgaaatcacc accactgccc ctgcgtttta tgccattttg
     1021 ccgttcacgc cgatcatcgg agtgctgatt tttgacggca aatggggtcc gcaattacac
     1081 atcatcacta ttctggtgat ttgtatgcta attgcctcca ttctggagtt catccgcagc
     1141 tttaataccc agaaagtttt ctctggtctg gaagtggctt atcgcggtat ggcagatgca
     1201 tttgctaacg tggtgatgct gctggttgcc gctggggtat tcgctcaggg gcttagcacc
     1261 atcggcttta ttcaaagtct gatttctatc gctacctcgt ttggttcggc gagtatcatc
     1321 ctgatgctgg tattggtgat cctgacaatg ctggcggcag tcacgaccgg ttcaggcaat
     1381 gcgccgtttt atgcgtttgt tgagatgatc ccgaaactgg cgcactcctc cggcattaac
     1441 ccggcgtatt tgactatccc gatgctgcag gcgtcaaacc tgggtcgtac cctatcaccc
     1501 gtttctggcg tagtcgttgc ggttgccggg atggcgaaga tctcgccgtt tgaagtcgta
     1561 aaacgcacct cggtgccggt gcttgttggt ttggtgattg ttatcgttgc tacagagctg
     1621 atggtgccag gaacggcagc agcggtcaca ggcaagtaaa tagtaatgcc ggaggcaagt
     1681 ttcctccggc tttatcatgt cacccactgt ggtatatgcg ttgcggtctg ccaactttgc
     1741 cgtgaacaat ttcagcaata atcagatggc ggctggcgca atattcaaga taacgcctgg
     1801 cagtggtgcg actgatggtc agtgcctgcg ccaccgtttc tgccgtatgt tgcacaccag
     1861 gctctttgaa cagttttcgc accgcgttta gcgtcaaggg gtcaatgccg gtcggcagtt
     1921 cgtctttggg ttcaccgcga gcataggcat taaacatctc atcaatttgc ttctggctgg
     1981 cgctatcaat actttccagc atatgtttac gctggcggaa acgggttagc gtttgcccca
     2041 accgttcata ggcaatgggc ttaatgagat aatcaaatac accacaacgt acggcttcag
     2101 acaccgtttc catatcgctg gcagcagtgg taaacaccac gtcgccggga taatgcgcct
     2161 gcaccagttc atgcagtaaa ttaatccctc taccgtcagg aagatagtta tcgagcaaga
     2221 ttagccccgg cttaaaacgc tcgatcatca ttcgggcctg cgccaggttt cccgccagta
     2281 atatctggct gaatccggga atgtgacgaa tatattccgc atgcatctct gccagcggcg
     2341 tttcgtcctc aacgatcaat agggttaatg gagctgtcat taggtttcac tttcggaata
     2401 tagattgaaa ataaggtacc gcagggatca ttatcttcga gagtgataac accaccgcag
     2461 cgcgttacgt agctggcaat caagtacaac ccaatgccat gttcaccggg ctcgtcagca
     2521 cgcgtactga caccctgctc aaatattttg tctcgtagag actctggaac gccgcagccc
     2581 tgatcggcga cttcaatcac cacatcatcg ccttcatcgc tgaggaataa ttcaacgatc
     2641 ttgtttcctt catcgctacg caggcttgct tcgaaggcgt tatcaagtaa attgccaaca
     2701 atggctgcaa actcggtgct gtccagtcct ggcggcagtt gcgaaagctg gctaccgggg
     2761 acgatgacca tttttagccc cagttcgcgg gcgcgctgca ctttaccaaa aagcagcccc
     2821 gccacctggc gatcggcaaa cgcctcacgc aggctgtcaa taagctgttg ctgggcctga
     2881 gactccccct gcaccatcgc cagcacgcga tcatactctt tcatctgcaa cagaccattg
     2941 agcgtcgaca tccaattgag atgctcgtgt cgcaatgtac gaaggctctc gacgtattgt
     3001 ttaatttgcg tcagttgcgc attgagggtg gaaatttcgt ctttactacg aaagctaata
     3061 atggccccca gtaaatcatc gccagaacga atagcttccc ggttggcaat aacgctcaga
     3121 ccgttaaagt tcgccaccac atcctgacgt ttttcatcaa tctgttcggt aaagaaatcg
     3181 gcgggcctga ccacttcagc aatgggttta cccaaccatt gccgtccggg ggaactcagc
     3241 cccagcatct ttcttgcgtt acgattgatg gcggtaatgt acccgtgcgg atccaccgca
     3301 atcagccctt cataaaccga actaaacagc gcctcttgct gacggactac gcgagcgatt
     3361 tgctttggct ccatgcccat catctgccga cggatatgcg cggccaggaa ccacgacagc
     3421 aacatcaaaa tccctaacag cacgacaaac acgcctgcca tcggtaataa aaactcagcc
     3481 cgccagctat cgattttact caccagatag ccaatcgaca ccacgccgat gacttttcca
     3541 tcgtcatcaa agattggcgt tttggcacgc atcgccatgc caattgaccc tttcccggtg
     3601 atgaagtagc tctccccttt ctccagcgcg cccggtttgg tgaactgcat aggataacca
     3661 attttctccg gattaggatg gtaaagacga atcgagtgcc gatcgccaat caccacataa
     3721 tcgaaatcag tatctctttg taatttgtca gcgatggtcg ccagcctctt gtagtcacgc
     3781 gttttcactg cactgatgat actgtcatta gaagcaataa tttttgcctg attcattgcc
     3841 atgtcgcgca catgaagcgt taaatagtcc tcaaaactgg ctgtaaaata ttgcgccagg
     3901 gctgcaatga caaatattga gaacaccaga atcagcaaaa agatacgcag cggaaatgcc
     3961 agtctttgga aaaatgcaaa ctgtttattc tctttaagct gcaacattgt tttccttgcg
     4021 ggttgtcccg agcctctttg acggatgtaa ttaaaatgtt gaatcaaaat acatagatat
     4081 taaatatgat cgtggcaaat tttattcatt aaatcaatta aatcacttaa acctgcgggt
     4141 tatttcgtaa ttacattagt taaaaaactt aaaaccatta aataaataaa aaaccactaa
     4201 ctctatgtga aataaatcaa aatttcacgc cgaaatactc cttaggatgt atagcgaaag
     4261 gagaaaaaga tatacctcga tcgcccccct ttctcccaag tgaaaataaa aggttatcaa
     4321 tttgcaacat tgaacaacat tcgttgcaaa tcgataacaa catgcacctt caggatacta
     4381 tttattatgt tcggcaatga tattttcacc cgcgtaaaac gttcagaaaa taaaaaaatg
     4441 gcggaaatcg cccaattcct gcatgaaaat gatttgagcg ttgacaccac agtcgaagta
     4501 tttattaccg taacccgcga tgaaaagctt atcgcgtgcg gtggaattgc cggaaatatt
     4561 attaaatgcg ttgctatcag tgaatccgtt cgcggtgaag gactggcgct gacattagcc
     4621 actgaactga taaacctcgc ctatgagcgg catagcacgc atctgtttat ttataccaaa
     4681 accgaatacg aggcgctgtt ccgccagtgc ggtttttcca cgctgaccag cgtacccggc
     4741 gtgatggtgc tgatggaaaa cagcgccacg cgactgaaac gctatgccga atcgctgaaa
     4801 aaatttcgtc atccagggaa caagattggc tgcattgtga tgaacgccaa tccctttacg
     4861 aatggtcacc gttatctgat tcaacaggct gcggcacagt gcgactggtt gcatctgttt
     4921 ttagtcaaag aagattcttc acgcttcccc tatgaagacc ggctggatct ggtgttaaaa
     4981 ggcaccgccg atattccacg cctgactgtg catcgcggct ccgaatacat catctcccgc
     5041 gctacgttcc cttgctactt cattaaagaa cagagcgtca ttaaccattg ttacaccgaa
     5101 attgatctga agattttccg tcagtacctc gctcccgcgc tgggtgtaac tcaccgcttt
     5161 gtcggtactg aacccttttg tcgcgttact gcccagtaca accaggatat gcgctactgg
     5221 ctggaaacac cgactatctc cgcaccgccc atcgaactgg ttgaaattga gcggctgcgt
     5281 taccaggaga tgccgatatc cgcttcccgg gtacgtcaac tgctggcaaa aaacgatctc
     5341 acggctatcg cgccgctggt ccctgcagtc acgctgcatt atttgcagaa cctgcttgag
     5401 cactcccgcc aggacgcggc agctcgtcaa aagacccccg catgagaaac aggtgaaaaa
     5461 tgaaaataaa ccagcccgcc gttgcaggca cccttgagtc tggggatgtg atgatacgca
     5521 tcgccccact cgatacgcag gatatcgacc tgcaaatcaa cagcagcgtt gagaaacagt
     5581 ttggcgatgc aattcgcacc accattctgg aagttctcgc ccgctacaac gtgcgcggcg
     5641 tacagctgaa tgtcgatgac aaaggcgcac tggactgcat tttacgtgca cgactggaag
     5701 ccctgctggc acgcgccagc ggtatcccgg ctctgccatg ggaggattgc caatgatttc
     5761 cgcttcgctg caacaacgta aaactcgcac ccgccgcagt atgttgtttg tgcctggtgc
     5821 taatgccgcg atggtcagca actccttcat ctacccggct gatgccctga tgtttgacct
     5881 cgaagactcc gttgcattgc gtgaaaaaga caccgcccgc cgcatggttt accacacgct
     5941 gcaacatccg ctttatcgcg atattgaaac cattgtgcgt gtaaacgcgc tggattccga
     6001 atggggtgtt aacgacctgg aagccgtcgt tcgcggtggt gcggacgttg tgcgtctgcc
     6061 gaaaaccgat accgctcagg atgttctgga tatcgaaaaa gagatcctgc gtatcgaaaa
     6121 agcctgtggt cgtgaacccg gcagtaccgg cctgctggcg gcgattgaat ctccgctggg
     6181 cattacccgc gcggtagaaa tcgctcacgc ttccgagcgt ttgatcggta tcgccctcgg
     6241 tgcagaagac tatgtgcgca acctgcgtac agaacgctcc ccggaaggaa ctgaactgct
     6301 gttcgctcgc tgctccattt tgcaggccgc gcgttctgcg ggtattcagg cgttcgatac
     6361 cgtctattcc gacgctaaca acgaagccgg attcctgcaa gaagccgccc acatcaaaca
     6421 gctgggcttt gacggcaaat cgctgatcaa cccgcgtcag attgatctgc tgcacaacct
     6481 ctacgcaccg acccagaaag aagtggatca cgcccgccgc gtcgtagaag ccgctgaagc
     6541 cgccgctcgc gaaggcctcg gcgtggtttc cctgaacggc aagatggtgg acggtccggt
     6601 tatcgatcgc gcccgtctgg tgctctcccg cgcagaactt tccggcatcc gcgaagaata
     6661 aggcaatcaa aatgacgcag aaaattgaac aatctcaacg acaagaacgg gtagcggcct
     6721 ggaatcgtcg cgctgaatgc gatcttgccg ctttccagaa ctcgccaaag caaacctacc
     6781 aggctgaaaa agcgcgcgat cgcaaactgt gcgccaacct ggaagaagcg attcgtcgct
     6841 ctggtttaca ggacggcatg acggtttcct tccatcacgc tttccgtggc ggtgacctga
     6901 ccgtcaatat ggtgatggac gtcatcgcga agatgggctt taaaaacctg actctggcct
     6961 ccagctccct gagtgattgc catgcgccac tggtagaaca cattcgccag ggcgtggtta
     7021 cccgcattta tacctctggc ctgcgcggtc cactggcgga agaaatctcc cgtggtctgc
     7081 tggcggaacc ggtgcaaatc cactctcacg gcggacgtgt gcatctggta cagagcggcg
     7141 aactgaatat cgatgtggct ttcctcggcg tcccgtcctg tgatgaattc ggtaatgcca
     7201 acggctactc cggtaaagcc tgctgcggct ccctcggcta tgcaatggtt gatgccgaca
     7261 acgcaaaaca ggtcgtgatg cttaccgaag aactgctgcc ttatccgcat aatccggcaa
     7321 gcattgagca agatcaggtt gatctgatcg tcaaagttga tcgcgttggc gatgctgcaa
     7381 aaatcggcgc tggtgctacc cgtatgacca ctaacccgcg cgaactgctt attgcccgta
     7441 gcgctgcgga tgtgattgtt aactctggct acttcaaaga aggtttctcc atgcagaccg
     7501 gcaccggcgg cgcttcgctg gcggtaaccc gtttcctgga agacaaaatg cgtagccgcg
     7561 atattcgcgc cgacttcgcc ctcggcggta ttaccgcaac gatggttgat ctgcacgaaa
     7621 aaggtttgat ccgcaaactg ctggatgtgc agagctttga cagccatgct gcgcaatcgc
     7681 tggcccgtaa ccccaatcac atcgaaatca gcgccaacca gtacgctaac tgggggtcga
     7741 aaggcgcagc ggttgatcgt ctcgacgtgg tggtactgag cgcgctggaa attgacaccc
     7801 agttcaacgt taacgtgctg accggctctg acggcgtact gcgtggtgct tccggtggtc
     7861 actgcgatac cgcgattgct tctgcgcttt ccatcatcgt cgcgccgctg gtacgcggtc
     7921 gtattccgac gctggtggat aacgtgctga cctgcatcac cccgggctcc agtgtcgata
     7981 ttctggtcac agaccacggc atcgctgtta acccggcacg tccggagctg gcagaacgtc
     8041 tgcaggaagc ggggatcaaa gtggtttcta ttgagtggct gcgtgaacgt gcgcgtctgc
     8101 tgaccggtga accacagccg attgaattca ccgaccgcgt cgttgccgtt gtgcgttacc
     8161 gcgatggctc ggtgatcgat gttgtgcatc aggtgaagga ataagccatg cacctgcttc
     8221 ctgaactcgc cagccaccat gcggtgtcaa ttcccgagct gctcgtcagc cgggatgaaa
     8281 ggcaagcacg gcaacacgcc tggctcaagc gccatcctgt tccactggtc tcctttaccg
     8341 tggttgcgcc tgggccgatt aaagacagcg aggtcacacg ccgaattttt aatcatggcg
     8401 tgacagcctt gcgtgcctta gccgcaaaac agggctggca aattcaggag caggctgcac
     8461 tggtttccgc cagcgggccg gagggcatgt tgagcattgc cgccccggct cgcgacctca
     8521 agctcgccac cattgagctt gaacatagtc atcctctcgg gcggttatgg gatatcgatg
     8581 tcctgacacc cgaaggcgaa attctctccc gccgcgacta ttcactgccg cctcgccgct
     8641 gcctgttgtg cgagcaaagc gcagctgttt gcgcgcgtgg aaaaacccat caactgaccg
     8701 atttactcaa ccgcatggag gcgctgctga acgatgtcga tgcctgcaac gtcaactaaa
     8761 aacacaaagc ttgcgacgtc attaatcgat gagtacgccc tgctgggctg gcgcgccatg
     8821 ctgactgaag tcaatctgtc accgaaacca ggcctcgtgg atcgcattaa ctgcggtgcg
     8881 cataaagata tggcgctgga agatttccac cgcagcgcgc tggcgattca gggctggtta
     8941 ccccgtttca ttgaatttgg tgcctgtagt gctgaaatgg caccagaagc ggtactccac
     9001 ggattacgcc caataggtat ggcttgcgaa ggtgatatgt tccgcgccac tgcgggcgta
     9061 aacacgcata aaggcagcat tttttcttta gggctactgt gtgcggcaat tggccgtttg
     9121 cttcaactca accagtcggt aacgccaata acaatttgtg cgacggcggc aagtttctgc
     9181 cgtggcctga ccgatcgcga actgcgtacc aataattcac aactgacggc aggtcaacgg
     9241 ttgtaccagc agcttggcct taccggcgca cgcggtgaag ccgaagcggg ttatccactg
     9301 gtgatcaacc acgccctgcc gcattacctc actctgctgg atcaggggtt agatcctgaa
     9361 ctggcattgc tcgatacctt gctcctactg atggctacca acggcgatac caacgttgca
     9421 tcgcgcggtg gcgagggggg cctgcgctgg ctacagcgcg aggcgcaaac attattgcaa
     9481 aaagggggca tccgaacccc cgccgatctc gattatctcc ggcagttcga cagggagtgt
     9541 atcgaacgaa atctcagtcc aggcggcagt gccgacctgc tgatccttac ctggttttta
     9601 gcacagattt aattatttaa gcacttgata aatttggaaa tattaatttt cggagaaccc
     9661 gtatgtcttt agcaaaagat aatatatgga aactattggc cccactggtg gtgatgggcg
     9721 tcatgtttct tatccctgtc cccgacggta tgccaccgca ggcatggcat tacttcgctg
     9781 tgtttgtggc aatgattgtc ggcatgatcc ttgagccaat tccggcaaca gcgatcagtt
     9841 ttattgcggt tactatttgc gttattggca gtaattacct tctctttgat gccaaagaat
     9901 tagctgaccc agcgtttaat gcgcaaaaac aggcgctgaa atggggtctg gctggttttt
     9961 ccagcaccac ggtatggctg gtatttggcg catttatttt tgcattaggg tatgaagttt
    10021 ccgggttagg tcgtcgtatt gcccttttcc tggtgaaatt catgggcaaa cgcacgctga
    10081 cgttaggtta tgcgattgtc attatcgaca ttctgctggc accgtttaca ccgtccaaca
    10141 ccgcgcgtac cgggggtacg gtttttccgg tcattaaaaa cctgccgccg ttgtttaaat
    10201 cattcccgaa cgatccgtcc gcgcgtcgta ttggcggcta tttgatgtgg atgatggtca
    10261 ttagtaccag tctgagttcg tccatgtttg tcaccggtgc ggcaccaaac gtgctgggtc
    10321 tggagttcgt cagcaaaatt gccggtatcc agattagctg gttgcagtgg ttcctctgct
    10381 tcctgccggt tggggttatc ttgcttatca ttgcgccgtg gctttcctac gtgctgtaca
    10441 aaccggaaat cacacacagt gaagaagtgg caacctgggc gggtgatgaa ctaaaaacca
    10501 tgggtgcgct gacacgcaga gagtggacgc tgattggcct tgtattgctc agcttaggtt
    10561 tgtgggtatt tggcagtgaa gtcattaatg ctactgcggt aggtctgctg gcagtttcgc
    10621 tgatgctggc gctgcacgtt gtgccgtgga aagacattac tcgctataac agcgcatgga
    10681 acacgctggt caacctggca acgctggttg ttatggcgaa tggcctgacc cgttctggct
    10741 ttatcgactg gttcgccaac acgatgagta cgcacctgga aggcttctca ccaaacgcaa
    10801 cggtgattgt actggttctg gtgttctact ttgcccacta cctgtttgcc agcctgtctg
    10861 cgcacaccgc aaccatgctg ccggttattc tggccgtcgg taaagggatt ccgggcgtac
    10921 caatggaaca actgtgtatc ctgctggtgc tgtctatcgg tatcatgggc tgtctgacgc
    10981 cgtatgcaac cggtcctggg gtgattattt acggctgtgg ctatgtgaaa tcaaaagatt
    11041 actggcgtct tggcgcaatc ttcggggtga tttacatctc tatgctgctg ttggttggct
    11101 ggccgattct cgccatgtgg aactaatacg ttcgccaaac gctgaataaa tcatcacgcc
    11161 cgccaggtaa gcccacctgg cgggcttttt tatgatttaa tagataatat tcagtatatg
    11221 tatgagttcc acacccatta tgaaagcatt ctggcgtaac gccgcgttgc tcgcggtttc
    11281 tctgcttccc ttctcttctg ccaacgccgt ggcgttgcag gcaaaacagt atggcgattt
    11341 tgatcgctat gtcctggccc tctcctggca aaccggattt tgccagagtc aacacgatcg
    11401 aaatcgtaac gaacgagatg aatgtcgcct gcaaaccgaa acgaccaaca aagctgattt
    11461 tctgaccgta catggtctgt ggccaggatt gcctaaatcg gttgctgccc gtggtgttga
    11521 tgaacgccgc tggatgcgct tcggttgcgc tactcgccca atcccgaatc taccagaagc
    11581 gcgcgccagc cgaatgtgtt catcgccgga aaccgggtta tcactggaaa cggccgctaa
    11641 actaagtgaa gtcatgccag gagctggcgg acgttcctgc ctggaacgct acgaatatgc
    11701 caaacacggt gcctgctttg gttttgatcc agacgcatac ttcggcacga tggtacgcct
    11761 gaatcaagaa attaaagaga gcgaagccgg aaaattcctt gcggataact acggtaacac
    11821 agtgagccgc cgtgactttg atgccgcctt tgccaaaagc tggggaaaag agaacgtgaa
    11881 agcagttaag ctaacgtgcc agggtaaccc tgcgtattta actgaaattc agatctcgat
    11941 caaagctgac gccatcaacg ctccgctttc tgccaactca ttcttgccac aaccacaccc
    12001 aggcaactgt ggcaaaacct ttgtgattga taaagcaggt tattaatcct ctgatttaca
    12061 aaacacgcta attcgtcttc cataaaccat gacgattaag atttatacat aatgcgctac
    12121 attgaggaat tccctgaatg tgacgcaaat cacttcaaag agtgagcaac agtcagtatc
    12181 atcatgtgag ttaaaccctc gccgcctgac ggtgagggtt ttcttttggg tatgtttatc
    12241 tgcgacactc gcagtaccga cgacatggag taaaaatgtc cagaccaact atcatcatta
    12301 acgacctgga tgccgaacgc atcgatattc tgctggagca acccgcctat gctggtttgc
    12361 caatcgccga cgcgttaaac gcagagttgg atcgcgccca aatgtgttcg ccagaagaga
    12421 taccacacga cgtggtgaca atgaacagcc gggttaaatt ccgcaatctt agcgatggcg
    12481 aagtgcgtgt gcgcacgctg gtgtatccgg caaaaatgac cgacagcaat actcagcttt
    12541 ccgttatggc tccggtaggt gccgcactgc tggggctgcg cgttggcgat tccattcact
    12601 gggaacttcc gggcggcgtt gcaacccacc ttgaagtgct ggaactcgaa taccagccag
    12661 aagctgctgg cgactacctg ctttaatccc atctgcacaa gcgataactc gctttgccca
    12721 gaggatgcac tcgcatcctc tttccgctca aatatcaacc agatttatca ttttgcgctt
    12781 aagcgcagca ttctaatctg aactggttat tcatctgcgg aacctcatat gagtgaagct
    12841 ctcggtatca ctgcatttta tatattcata gccgcaatta ttgttgctgc agtgctatac
    12901 cttgaacaac gctggtagtg cctggtttta tttattttcc agctcaatag caaattcaga
    12961 tcaaatcagc ctcgcaaatt atagattcat aaccaataat gtgctgttat gtatacatgc
    13021 aaaaacaagg agagaatgct tatgtacaaa acaatcatca tgccagtgga tgttttcgaa
    13081 atggaattga gcgacaaagc ggttcgccac gctgaattcc tcgcccagga tgacggagtt
    13141 attcatctac ttcacgtact accggggtca gccagcctga gcctgcaccg ttttgccgct
    13201 gatgtacgtc gttttgaaga gcatctgcaa catgaagcag aagaacgttt gcaaacgatg
    13261 gtcagccact tcaccatcga tccttcccgc attaaacaac atgtccgttt tggtagcgtg
    13321 cgggatgaag tcaatgagtt ggcagaagaa ctgggggctg atgttgtcgt tatcggttcg
    13381 cgcaacccat cgatttcgac ccatctgtta ggctctaacg cctcaagcgt tatccgccac
    13441 gccaatctgc cggtgctggt cgttcgctaa tagataaaag tatttatgtc gccctgcccg
    13501 gagccatccc tgcagggctt tttaatgcaa taaaaaagcc gccaggtttg accccggcgg
    13561 ctaagcaatt gcaggtgaat cttacttctt cttatgcagt tttggtgcga atcaggtagt
    13621 caaaagcact cagagaggct ttggcacctt cgccagtggc gatgatgatc tgcttgtacg
    13681 gaaccgtcgt acagtcaccc gctgcgaaca cgcctttgac gttggtttcg catttcgcat
    13741 caatgataat ctcgcccatg cgattacgtt cgactgcgcc ttccagccag ttggtgttcg
    13801 gcagcagacc aatctggacg aaaataccgg ccagttcgat gttgtgaata tcgccgctga
    13861 cacgatcgcg atattccaga cctacgactt tgctaccgtc gcctttcact tccgtggttt
    13921 gcgcattcag aataatgtcg acgtttttca ggctgcgtag tttgtcctgc agaacctggt
    13981 cggctttcat ttctggcgca aattccagca gcgttacgtg ctcaacgata cccgccaggt
    14041 caattgccgc ttccacgccg gagttaccgc cgccgataac cgctacgcgt ttgcctttaa
    14101 acagcgggcc gtcgcagtgc gggcagtagg tcacgccttt ggtgcgatac tgatcttcgc
    14161 caggaacgtt catgttgcgc cattttgcac cagtcgccac gataatgctg cgtgctttca
    14221 gtaccgcgcc agaagctgtt tcaatctgat gcaggccgcc ttcaaccgct gccgggatca
    14281 gtttgctcgc gctctggctg tcgatcacat caacgtcgta ttcatcaaca tgaactttca
    14341 gcgcacctgc cagtttctgg ccttcggtct tcggtacaga aatgtagttt tcgatatcaa
    14401 cggtatcgag gatctgacca ccaaaacgtt cgcccatcag accggtacgg atgcctttac
    14461 gtgcggagta aattgctgcc gctgcacccg ccgggccgga accaacgatt aatacgtcat
    14521 aagcatcacg cttgttcagc tcttctgccg cacgtttttc cgcaccagta tcaattttgg
    14581 caacgatttc agtcaacgtc atgcggccct gaccaaactc tttcccgttt acgaacactg
    14641 ccggaacgcc catcacgttg cgatcggtga tttcgttctg gaaggtgccg ccgtcaattg
    14701 cagtgtgctt gatgcgcggg ttcagtacgc tcatcaggtt cagcgcctgc accacgtccg
    14761 ggcagttgtg gcaagagagc gagtaatagg tttcgaattc aaaatcaccg tcaatatggc
    14821 gaatctgctc cagcagagac tgcgcttctt tcgacggatg accaccggtc cacagcaacg
    14881 ctagtaccag cgaggtgaac tcgtggccca gcggggagcc tgcaaaacgt ggcccctggt
    14941 tagaacctgg gttggtgatc aggaaagacg gcttacgcac cggcaagctg ttatcttctt
    15001 taaaggtgac tttgtctgac agttctgcga tttcagccaa cagttccttg atttctgccg
    15061 atttagcgct gtcatccagc gtggcaatta actcaacagg cttggtcaat ttctcaaggt
    15121 aagccttgag ttgagttttc atatttgtgt cgagcatgtt tatctcctgg gctttaaaca
    15181 tcatcatgca agcggcctct gcggctacct ggacgcagct tgcatcatgg tgcggggaag
    15241 aaaatcgggt gccgcagcac ccgaagaatt aggtgaattt ccgaagtatc tcgcgccgga
    15301 gttgatagcg cgaaagacga aggaaattta gattttacca accaggtcca gagacggagc
    15361 cagagttgct tcaccttctt tccatttagc cgggcaaact tcacctgggt gagaagctac
    15421 gtactgtgct gctttgattt tacgcagcag gtcagacgcg tcacggccaa tgccttcagc
    15481 ggtaacttcg attgcctgga tgataccctg cgggtcaaca acgaaggtcg cacggtcagc
    15541 cagaccttca tcttcacgca tgttgtcgaa gttacgggtc agggcgccag tcgggtcgcc
    15601 gatcatcgca tatttgattt tagcgatggt ttcagagctg ctgtgccatg ctttgtgggt
    15661 gaagtgagta tcggtagata ctgcgtatac gtctacgccc agtttctgca attcttcgta
    15721 atggtcagca acgtcaccca gttcggtcgg gcatacgaaa gtaaagtcag ccgggtagaa
    15781 gaagaagacg ctccagcggc cttcggtatc tttttcggtg atttcgatga attcgccgtt
    15841 tttgaatgcc tggtttttaa aaggtttaat tttggtgtta atcaaggaca tctatacttc
    15901 ctccgtgttt tcgatgagat gtaaggtaac caatttttgc cgattcggct aatgcgtttc
    15961 cattatcaaa tcgataagtt ttaccttaca accttcgtaa gacaactttg tgaactttgc
    16021 accgccagcg gcgataaaaa gtaaaatggc cccctttcca ggtgaccgtg taatctgaac
    16081 taccctttgg tatcttcagc gcctgcctgc aggctgaggt gttgccctta caaatgcaac
    16141 aacgacatgg attacaacac cctcataaac aaagggcaat cacctgatct aagctcttac
    16201 ctatgacagt gataggttat gccttttact cgacttttgc actgactgaa aaggacaaat
    16261 taatgttaaa aaagatactt ttactggctc tgcttcctgc aatcgccttc gcagaggaac
    16321 ttcctgcccc agtaaaagcg attgaaaaac agggtattac aatcatcaaa acgttcgatg
    16381 cccctggagg aatgaaaggt tatctcggta agtatcagga tatgggcgtc accatctacc
    16441 tgactccaga tggtaagcac gctatctctg gttacatgta caacgaaaaa ggtgaaaacc
    16501 tgagtaacac gcttatcgaa aaagaaattt acgcacccgc cggacgtgaa atgtggcaac
    16561 ggatggaaca atcccactgg ctcctcgacg gtaaaaaaga tgcgccggtc attgtctacg
    16621 tcttcgccga tccgttctgc ccatattgta aacaattctg gcagcaggct cgtccgtggg
    16681 tagattctgg caaagtgcaa ttgagaacat tgttggttgg ggttatcaag ccagaaagcc
    16741 cggcgacagc agcggcaatt cttgcctcca aagatcccgc aaaaacctgg caacaatatg
    16801 aagcctctgg tggcaagctt aagctaagcg tgcctgcaaa cgtaagcaca gagcaaatga
    16861 aagtgttaag cgacaacgag aaactcatgg acgatctggg ggcaaatgtc acaccagcta
    16921 tctattacat gagtaaggaa aataccctgc aacaagccgt cggattaccc gatcagaaaa
    16981 cacttaatat cattatgggg aataaataga atataattca tcgatggttg aaatctctct
    17041 ctgcttttca accatcctaa aaacgcatga attgctcgta acccacctga cagctatgct
    17101 attttagaaa atcttaatcc aaagttaagc agaaaaacat aatacatata aatacaacaa
    17161 cattattgat caagtgaatg atttatggaa gagacaatac aacggagtat gttatggcta
    17221 atctttatga tctaaaaaaa tttgacctca atttactggt catatttgaa tgtatttatc
    17281 aacatctcag cattagtaaa gcggcagaat cattatatat aacgccctct gctgttagtc
    17341 agtcgttaca acgtttacgt gcgcaattta atgacccgtt attcattcgc tcagggaaag
    17401 gtatcgcgcc gactacaaca gggttaaacc tgcatcacca tcttgagaaa aatctcagag
    17461 gccttgaaca aacgatcaat atcgttaata agtctgaact aaagaaaaat tttattatct
    17521 acggtcctca gcttatttct tgtactaata acaacatact aatccgctgt cttagacaag
    17581 atacttcaat cgatatagaa tgccatgaca tactgctttc agctgaaaat gccgaagaac
    17641 tgttggttca acgcaaaact gacctggcaa ttacgctgca accagtaatt agtcgctctg
    17701 tcatctgcat gccgttacac actattcgca attcccttat atgtagcaac aaacatcctc
    17761 gtattactga taccagtaac tatgaacaaa ttatcgccga agaattcacc ctattgatat
    17821 ctaaatcagc agggattgac gaaattcaaa tggacataga tgaacgtttt atgaaccgta
    17881 aagtttcatt tcgcagttct tctttgatga ctataattaa tagtatttct gttactgact
    17941 tattaggcat tgtgcccatc gaactgtacg atttacaccg tgattttttg aagttaaaag
    18001 aaataaaact ggaacaacct cttcctgcgg ttaaacttta tatttcctat aacaaggctt
    18061 cgttgaacaa ccttgtcttc tcccgattca tagatcgttt aaatgatagc ttctgattca
    18121 ttaccactga caaataatat attacgcatc aattaattca ttgtgtgaat ataaaataat
    18181 gtgctctatc ttgcttcgtt tatcctataa ttagcggatt catctcacga atcaccccgt
    18241 aacgttcaga gagcgttttt agatgtctat ttataaaatc ccgcttccac tgaatattct
    18301 ggaagctgca aaagaacgta ttacctggac acttaatacc ctaccccgta tctgtgtttc
    18361 tttttcgggt ggtaaagatt ccggattaat gctgcatctg acagctgaaa ttgcccgaca
    18421 aatgggcaaa aaaatctgcg ttttgtttat cgactgggaa gcgcagttct catgcactat
    18481 taactatgtt cagtcattac gtgagtttta cgccgatgtc atcgaagagt tttactgggt
    18541 tgcgctcccg cttacgacgc aaaattccct ttctcaatac caacccgaat ggcagtgctg
    18601 ggaacctgat gtcgaatggg tgcgtcagcc cccgcaagat gcgataaccg atcctgactt
    18661 tttctccttt taccagcctg gcatgacctt cgaacaattt gtgcgtgagt ttgctgaatg
    18721 gttttcacaa aaacgtccgg cggcgatgat gatcggcatc cgtgcagatg agtcctacaa
    18781 ccgttttgtc gccatcgcca gtttaaataa acaacgtttt gccgatgata agccctggac
    18841 caccgccgca ccaggcggtc atagctggta catttacccc atctacgact ggaaagtggc
    18901 tgatatctgg acctggtatg ctaaccatca acagctctgt aacccactgt ataatataat
    18961 gtatcaggca ggcgtccctc tgcgtcatat gcgaatttgc gaaccttttg gcccggagca
    19021 acggcaagga ttatggctct atcacgttat cgaaccggat cgctgggctg ctatgtgcgc
    19081 acgagtcagc ggggtaaaaa gtggcggcat ttacgccgga catgataatc atttttatgg
    19141 tcatcggaaa atcctcaagc cagaacattt agactggcaa gaatatgcat tattgctgct
    19201 caatagcatg ccggaaaaaa cggctgagca ttaccgcaat aaaatcgcta tttatttgca
    19261 ctggtatcag aaaaaaggca tcgaagttcc acaaacccag caaggggaca ttggcgcgaa
    19321 agatatcccc tcctggcggc gaatatgcaa agttttactc aataacgatt actggtgccg
    19381 ggcattatca tttagtccta cgaaagcgaa gaactatcag cgttataacg aacggataaa
    19441 aggaaaacgt caggaatggg ggatactatg caacaacgat taacgcagga tttaacccaa
    19501 tttctcgcca gcttgccgga agatgatcgc atcaaagcga ttaatgaaat ccgcatggcg
    19561 atccatcagg ttagcccctt tcgtgaagag ccagtggatt gcgttctgtg ggttaaaaac
    19621 agccaactta tgcctaatga ttacaaccca aacaacgtgg caccacccga aaaaaaactg
    19681 ctgaaaaaat ccattgaaat agatggtttt acccaaccca ttgtggtcac gcatacggat
    19741 aaaaatgccc tggaaatcgt ggatggtttt caccgccatg aaattggtaa aggctcatca
    19801 tcgttaaaat tacgcttaaa aggttatttg ccggtgacct gtctggaagg tacgcgtaat
    19861 caacgcatcg ccgcaactat tcgtcataat cgcgctcgtg gacgtcacca aatcactgcc
    19921 atgtcagaaa tcgtccgaga actcagccag ttgggatggg acgataataa aataggcaaa
    19981 gagctgggaa tggacagcga cgaagtgtta cgcctgaagc aaattaacgg cctgcaagag
    20041 ttgtttgccg accgtcaatt ttcccgcgcc tggacggtta aataactaca gctggcgcag
    20101 gcgttctgct gctgccagca acgtcgattc cttcttggca aaacagagac gaatcagttt
    20161 atgtgggaag ggatcggcgc aaaacaccga cagcggaatc gccgctacgc cgtgctcccg
    20221 cgtcagccac tggcaaaact caacatcatc cagcgtagat acggcgctgt aatccaccag
    20281 caaaaagtaa gtaccttcgc acgctaagat ctccagccga ctctcactta aggcattcac
    20341 cagaatatcg cgcttctggc gataaaattc cggtaacgca agatagtgct caggttctgc
    20401 atgtagcata tcggcaatcg ccagttgcgc cggggtattc accgaaaagg tcagatactg
    20461 atgcactttg cggatctcgg cgctgatggg cgcaggtgca acgcaatagc ccactttcca
    20521 gccggtcata tgataggtct tgccaaacga tgacaccgcc accgctcgct cacgcaattg
    20581 cggatgcgcc agcacgctgg catggccctg ttgtgaaaaa ttgatgtgct cgtagacttc
    20641 atcgctaatg acaaaaatct cgtgcccggc gatcgcctgc cacagagcgg cgaaatcagt
    20701 ctgctgccag acagttgcac tggggttatg cggggtgttg aggatcacca gtcgggtgcg
    20761 ctcgcttaac aatgcggcaa attcctgcca gtcaacgcga aaatgcggcg gttgcagtgc
    20821 aatacgtttc actattcccc ccgaaagtgc gatggcgggg gcgtaactgt catagctggg
    20881 atcaaaacaa atcacttcgt cgccattgcg caccaatgcg gttatcgccg cgtataacgc
    20941 ctccgtcgcc cctgccgtca cggtgatatt gctgtcgaca tccggttgat agccatacag
    21001 acgttccgtt ttctgagcaa tcgcctcgcg caaggcctgc acgccggtca tgggcgcgta
    21061 ttggtttgcc ccctgggcaa cgtggtacgc cagccgctcc tgtaaatagc gcggaccatc
    21121 aaaatcagga aagccttgcg aaaggttaat cgcctggtgt tgctgcgcca gcgcgctcat
    21181 ccgggtgaaa atagtggtgc caagttgtgg aagtttgctt tgtggaatca gagggttatt
    21241 tgtcattttt attgtacctg acgaagttgc gtgttgaagg cactataaca cgatgctagt
    21301 atttggcaat caagacgttt agatgtctaa ataaaacaat aaggacaaca caacatgcct
    21361 cacaatccta tccgcgtggt cgtcggcccg gctaactact tttcacatcc cggaagtttc
    21421 aatcacctgc acgatttttt cactgatgaa caactttctc gcgcggtgtg gatctacggc
    21481 gaacgcgcca ttgctgcggc acaaaccaaa ctgccgcctg cgtttgaact gccaggggta
    21541 aagcatattt tgtttcgtgg tcattgcagc gaaagcgatg tgcaacaact ggcggcggag
    21601 tccggtgacg atcgcagcgt ggtgattggc gtcggcggcg gcgcactact cgacaccgcg
    21661 aaagccctcg cccgccgtct cggtctgccg tttgttgccg ttccgacgat cgccgctacc
    21721 tgcgccgcct ggacaccgct ctccgtctgg tataacgatg ccggacaagc gctgcattat
    21781 gagattttcg acgacgccaa ttttctggtg ctggtggaac cggagattat cctcaatgcg
    21841 ccgcaagaat atctgctggc ggggatcggt gacacgctgg cgaaatggta tgaagcggtg
    21901 gtgctggctc cgcaaccaga aacgttgccg ctaaccgtgc ggctgggaat caataatgcg
    21961 caggccattc gcgacgtctt gttaaacagt agcgaacagg cgcttgccga tcagcaaaat
    22021 cagcaattaa cgcaatcatt ttgcgatgtg gtggatgcca ttattgctgg tggtgggatg
    22081 gtcggtggtc tgggcgatcg ttttacgcgt gtggcggcgg cccatgccgt gcataacggt
    22141 ctgaccgttt tgccacaaac cgagaagttt ctccacggca ccaaagtcgc ctacggaatt
    22201 ctggtgcaaa gcgccttgct gggtcaggat gatgtgctgg cgcaattaac tggggcgtat
    22261 cagcgttttc atctgccgac tacgctggcg gagctggaag tggatatcaa taatcaggtg
    22321 gagatcgaca aaatgattgc ccacacccta cgtccggtgg aatccattca ttacctgcca
    22381 gtcacgctga caccagatac cttgcgtgca gcgttcgaaa aagtggaatc gtttaaagcc
    22441 tgacgtacaa atttagcagc agcgcgcgcc gccttttcca ccgtagcgcg cgtcctgccg
    22501 ctcacggaaa aactcctcgt aggtcatcgg cgtttgatcg ggatggttaa cccgcatatg
    22561 ttcgacatag ttgtcgtaat caggcatacc aatcatcagt ttcgccgcct gacctaaata
    22621 ttttccggct tttgccagtg aatcaaacat ctaaacctcc ggtaatacaa atgccctcct
    22681 gaattaagag ggcttaattg ctatcaacac gctcgggctg agagtaaggg gagcgctcag
    22741 cacattcccc ctcactctga ctttagtgcg cgcctttcgc ctgcgccacg atctcctcga
    22801 cattttccgg catcggctca tatggcgttt ctttcgccgt tggcttcggc tctttcaatg
    22861 ccgccagtgc cgtcttaatc gagaacagcg ccagaaccac cacaaccacc ataaagaaga
    22921 tggttaatcc ggcatccaga cggttgttga acaccagttg cgccagttgt gactcagtat
    22981 actgcgacgg aatattgccg ctgtcgatca ttgcctggaa cttgttagca atcgccagga
    23041 atccgacttt cgcatccggg ctaaacgctt tctgccagcc tgcggtcagg gtacaaatca
    23101 gcagccaggc cgttggtacc agcgccaccc aggcgtaacg ttgacgtttc attttgaaca
    23161 gcaccacagc acagagcatc agcgccatcc ctgccagcat ctggttggca ataccaaaca
    23221 gctgccacaa ggtgttaata ccgcccagcg gatcgaccac gccctgatgc aggaagtagc
    23281 cccacgccag cacgcacagc gccgttgcca gcaggttagc aggcagtgaa tcggtccgtt
    23341 tcaggcccgg agacaccacg cccagcagat cctgcaacat aaagcgcgca gcacgcgtac
    23401 ccgcatccac cgccgtaaga ataaacagtg cttcaaacag aatggcgaag tgataccaga
    23461 acgccacatc catcatgccg cccagcgcac cgtgcagaat gtaggccatc cccaccgcca
    23521 gggtcggcgc accgcccgca cgggaaatga tcgactgttc acccacttcg ctggcaatct
    23581 ggtttagcgt atccggggta atcgcaaagc cccagctact caccacctgc gcggcagaag
    23641 caaccacatc cgccgtccct gccggagcca gcacagccat cgggctgttc atggcgaagt
    23701 acacacccgg atcgatgata caggcggaaa ccagcgccat aatcgccacg aaggattcca
    23761 ttaacatccc accgtaaccg ataaagcacg cctgcccttc gttcgccagc atcttcggtg
    23821 tagtgccgga agagatcagt gcatggaagc cagacaccgc gccacaggcg atggtgataa
    23881 acaggaacgg gaacaggtta ccggtccata ccgggccagt gccatcgaca aatttagtta
    23941 gcgcaggcat ggtcagcgtc gggcgcataa tcaaaatgcc caccgccaga ccgacgatag
    24001 tcccgatttt caggaaggtt gagaggtagt cacgcggagc cagcagcaac cacaccggca
    24061 gcaccgccgc cacaaaaccg taacccacca gcatccaggt cagctgcacg ccggtaaagt
    24121 caaagtacgg tgcccacgtc gggctttctg ccacccagcc gccagagata atggcgaaaa
    24181 taaggaacac cagaccaatc accgacactt cgccaatacg ccccggacgc aaatagcgca
    24241 ggtagatccc cataaacagc gccagcggaa tggtgaacgc aacggtatac gttccccacg
    24301 ggctatgagt cagggcttta accacgatca tcgccagcac ggcaaggata atgaccatga
    24361 tcataaagca ggccaccagc gctatcaccc cggcggttgg ccccatctct tctttgacca
    24421 gctcacccag cgagcgaccg tcacggcgcg tggaaacaaa cagcaccatg aaatcctgca
    24481 ccgcaccggc gagaaccacc cctgcgagca gccagatcat ccctggcagg taacccattt
    24541 gcgccgccag taccggccca accaacggac ctgctccggc aatggccgca aaatggtgac
    24601 cgaacagcac ttttttgtcc gtcggcacat agtccagccc gtcgttatgg cgcaccgctg
    24661 gcgtcatacg cgtcgggtca accgccagca catttttggc gatatacagc ccataaaaac
    24721 ggtaagcgat cagatagata cagaccgacg ccaccacaat ccacagcgcg ttgatctgtt
    24781 ccccacgatt taaagcaatg tatcccagag caaatgctcc cattacagag agcactgtcc
    24841 agacgaggta tttccctgat ttgttcatag ttgttatccg tgtgcgtgtc catagagatg
    24901 ttacattttg tttctataac atctttgtac aatttaacaa cacagcaacc atgcaaatcg
    24961 gagttgaaac caatatttaa cttaacaatg ttaactcgat cactccgtta accgaatttt
    25021 cctgcaatct cattttattc tgtcgggttc atcccaaaac cgctgtgccc agccgacagg
    25081 tgcagcaacg ccgcccctgt tcatcgaaaa cgacgatttc ccagctctga ttttgtcgtc
    25141 caagatgcag cggctggcag acaccgcgca cctttccctc agacaccggg cgatgatgtg
    25201 tggcgttaag ttccgtgccc accacacact gtccgtcgcg ggtcatcata aatccggcca
    25261 tcgatcccag cgtttccgcc agcgccgccg acgcgccgcc atgcagcagg ccaaacggct
    25321 gatgagtacg ggtatcaacc ggcatttcgg cttcgagtac atcatcgccc agacgggtat
    25381 acacaattcc cagatgcgcc accattgtgt tatcgctggt ggcgttcagc tcgtcgagcg
    25441 ttaaatggcg tttccagatc atgcttatgc ccccagcgtt gagccgccat cgaccacaat
    25501 atcctgtagg gtaatatggc tggcgaggtc agaggcgagg aacaaaatcg tgttggcgat
    25561 ctcctgcgga cgggcgattt tccctagcgg aatgccgagt ttaaactgct cgccaaagcc
    25621 gcgaatacgc tgctcttcgg cgtcatcgct cacccacagc gtgcgttgca tatcagtgtc
    25681 ggtggagccc ggcgaaacca cattacagcg cacgccgcta cccgccagtt ccagcccgac
    25741 gctcaacgcc aggcttttca gcgccgcttt cgatgcgcca taagcactca tgccaatgcg
    25801 cggcgtgtgt gcggcgtcgg acgcaacagt aacaatcgcc ccgccccgct gatggcgaaa
    25861 ctggttcatg gtctgctgaa acaggttaaa cgcaccgccg acgttgaccg caaaagtctg
    25921 ctgccagtcc tctttactga gctgatcggt cgcacccatg cgcaaaattc ccgccgcatt
    25981 gaccagcacg tccagtcgct ccgtttcagc taacagtcgc tgacatacct gcgccacctg
    26041 cgcagcatcg gcaacatcca ttacttcggt cgcaaaagga tattgctcct gagtaaacgc
    26101 ttgatcaaaa cctgtaactt tcgcccccgc ctcaacaaat gccagcgccg tggcgtagcc
    26161 tatacctttc cccgcgccag ttacccagac atttttaccg ctgaaatcca ttatttcacc
    26221 tcgcgggaga gtagcttcca ccaggcgtcg atggtcgggt ttttcgccag catgacaaag
    26281 tcgatatcgc catgcacttt gcgccagcgc gccgccagcg ccatcatgcg caccgaatcc
    26341 agaccgtagt cgatcaggtt gtcgtcatcg aagggttcat cggactcgtc cagcaacggc
    26401 aggatcacct cacgcagctc cgctttgctg gcggggatag gtgctggcag caattcttcg
    26461 gtcatcacca cccgaccaga acgtccggcc acatatttca gcgacatcaa atgctcgtca
    26521 cggctgaaat cggccagcgc atccgccacc ataaacggtt taatatcgcg cataaatgcg
    26581 tccgttgcgg tggtcataca gccaatgtgg gcatataccc cggtaataat cagctggtta
    26641 cgtccactct ctttgagcat ttgctccagc ggagaacgat gaaatgcgct gtagcgccat
    26701 ttcaccagca ccgtgtcgtc ggcatctggc gtcaggcgat ccaccacctt ttgctgttcc
    26761 ggcgaacggg tcaggccagg cccccacata tcattcaaca gcgcccgatc ttcatcgctc
    26821 tgctctttcg gctgggcggt gtaataaacc gggatattgt gctgtttgca gtagtcgcgt
    26881 agtgccgcaa tattcgcgat cacctgctcc atcatcgggc agttctcacc ccagaagctg
    26941 acaaaatagt cctgcatatc atggattaac aacgcggcac gttgcggttc aaaggcccag
    27001 tcgactttat tctgcggaat atcatgaggc tccggcagtg cgtaagcctg taattttgga
    27061 atagccatcg tgttctctcc ttcaggctga tgtgcgtgac gccagccact gacgtaattg
    27121 ttttttatcg actttcccga ccgccgtcag cggaagtgaa tctacacact ccacgcgatc
    27181 cggtaattta aattcggcaa taccctgttc acgcaggaaa cgacgcacct gcaccgcgcg
    27241 cagcggctct tttaccacca gataagcaca gcttttttcg cccatcagct catcttccat
    27301 gctcaccagt gcggcgtaga tcaccgccgg atggcgcagc agcaggtttt cgatctcttc
    27361 ggcagcgatc ttctcgccgc cacggttgat ctgatctttc tcgcgcccct gcacagtgat
    27421 gtaaccctct ggatcaatag agatcagatc gccggaacag taaaaaccgt tggcatcaaa
    27481 ggcgctggca ttgtgctgcg ggcttttgta atagccacgg aaggtgtacg gcccgcgcgt
    27541 catcaggcgt ccaacttccc cttgcggcag tggatttcct tcggcatcgg caacccatac
    27601 ttcatcgtcc gggcacattg ggtaaccctg ggtatggata attttctccg cgctatcatc
    27661 aagtcgagtg tagttcacca gcccttccgc catgccaaac acctgctgca actggcagcc
    27721 aatctcagcg ggaatacgcg ccgcaagggt ggcagaaaga cgtgcgccgc cgacctgcaa
    27781 cagtttcagc gaggcaagct gcgcccggct ttcaccttcg gtcagcgcct gcaaccacag
    27841 gctgactgcc ggtggcacca gcgcggtgac gttcacctga tgtttttcaa tcaatgggaa
    27901 gcaaagcgtg gcgctaggat cggcagccag aacgacagtg ccgccagcga gaaatacccc
    27961 caacgatccc ggtgaactca tggcgtagtt atgagccgcc gggatcgcgc acaggtagcg
    28021 cgtctgttgt gtgaactgac aaatctcgac gctacgacgc acgctgtagt agtagtcgtt
    28081 atgagtgcgc gggatcagtt tcggagtgcc ggtggtgccg ccggaaagct ggaaataggc
    28141 cacttcatca gcaggagatg gcgtggcagt aaaatcctca gccggatggt taatcgcatc
    28201 ctgcaagtta tgctcaccgc tgtcgttgag cagctgcacc acgcgaatgg aagaatgctc
    28261 tgcaacaaat gtgttgagga aatcatcccc gctaaatagc gcatgttggc gatcggcaat
    28321 cagtaacgcc ggttcaatct ggctggcata ggcgttcagt tcactacgct gatggctgaa
    28381 cagcgccagc accggcgcaa cgcccagttt cagcagcgcg aaaaaggtaa tgtaaagttc
    28441 agcgacgtta cccagttgta ccagcgcggt ttcaccaggt ttaatgccct gacggcgcaa
    28501 actacaggcg aggttatccg ccgcctgatt cagctcccgg taactcaact gccactcgcc
    28561 gtcgatcacc gcgatgctgt cactcgcagc gtggcgagtc agaatgtcgg tcagtggcaa
    28621 atcctgccag tagccttttt cccgatagcg acgggcaaac tcttccggcc atcgggtgaa
    28681 tggaatgctc atcctcgctc cttaatgcaa tccaaaaacg ttcaacatgg tagaaagttt
    28741 gacgcctgtt tcgcgccact cacccaacgg tgaggacgca ggcacaatcc ccgctccggc
    28801 aaacagacgc acctgatttt cccgcagctt cgcgcagcgg atggtcacca cccattcacc
    28861 gttaccttcg ctgtcacacc aacccacaat gccgccaaac agttcgcggt cgaacggctc
    28921 cagttcagca ataacctgag tcgcggccag atgcgggaaa ccgctcagcg caggagttgg
    28981 atgcagcaga caggccagag tcagtgcgtt ttcttgcgaa ttcgctttac cttcaaaggg
    29041 agttgcgaga tgccacagcg tcggcgtggt aatcaattgt ggagaggagg gaacgtgtaa
    29101 ctcactactg cgttcgcgca gtacctcttt catcgcctga gtcaccagtt catgttcatg
    29161 gcgatctttt tctgacgcca gcagacgatt acccgcttcg cgatcgagca cttcatccgg
    29221 ctgacgacgc gcggaaccgg ctaacggaat ggagctaaaa cgctcgccgt ctttacgtag
    29281 cagcagttcc gggctggccc ccagcagaac gccaccatca gccaacggaa catggaagtt
    29341 gtaactaacc gggttttgcg caatcaaccg ttccagcaat acgccactat caatggcggc
    29401 gtcagtggtg atatcaatca accgtgacaa caccactttg tcgacctgcg gcgtggcggt
    29461 aagtgcggca gcacgggcaa ccatctgttc aaacgtggtt tgttccggaa tggcctgacg
    29521 ttccaccacg ttcagcgact ggctgcgagt gaaacggcgt gcggaagctt gtttttcctg
    29581 acgggagaac gactgccagg attcgggtat atacagcgac gaaggctgac gtggatcgaa
    29641 gggaatcgcc ccgaccatca ccggattttt gatgccctgc gctttggcat cggcaaacag
    29701 cgcggcgagt ttttgctgga agggactgtc gggcgaatcc ccgttcacag ccggttcatc
    29761 gaagcgggcg aaacatcctg acgtcgtaaa actgcggtac ggcgacataa agaaaaagcg
    29821 attgggcgca agtgttgcca tggtctgctg tacttcctca gccagtgacg tatccatatc
    29881 atcctccaca aaatgataaa ggctttaata atgattatca tttatatttt cggtcgctaa
    29941 cctaaaagca aacgcgcact atgtcaactc ttgaggtaac gcaatctgca actcggggtt
    30001 gcatctggtt gtggttacaa tgaaaatgag aagcattaac ttcattaata acaggaagct
    30061 gacttgtgag actcgccccg ctctaccgca acgcccttct attaacagga cttttgcttt
    30121 caggaatagc cgcagttcag gccgccgact ggccgcgtca gattactgac agccgtggca
    30181 ctcataccct ggaaagccag ccgcagcgta ttgtttccac cagcgtcacc ctgaccggct
    30241 cactgctggc gattgatgct ccggtgatcg ccagcggcgc gaccacgcca aataaccgcg
    30301 tcgcggatga ccagggcttt ttacgccagt ggagcaaggt ggcgaaagaa cgcaaactgc
    30361 aacggctcta tatcggcgaa ccgagtgcag aagccgttgc cgcgcaaatg ccggatctga
    30421 ttttaattag cgcaaccggc ggcgattcgg cgctggcgct gtacgatcag ctttctacca
    30481 tcgccccaac attaatcatc aattacgacg acaaaagctg gcagtcgctg ttaacgcagc
    30541 ttggggaaat taccgggcat gagaaacaag cggcagagcg gattgcacag tttgataagc
    30601 aactggcagc ggcgaaagag caaatcaaat taccgccgca gccggtcact gcccttgtct
    30661 ataccgccgc tgcacacagt gccaatctct ggacgccgga atcagcacaa gggcagatgc
    30721 tggaacaact cggctttacg ctggcgaagt tgcccgcagg cttaaacgcc agccaaagcc
    30781 agggcaaacg ccatgacatc attcagcttg gtggagaaaa tctggctgca gggttaaatg
    30841 gcgagtcact attcctgttc gctggtgatc agaaagacgc cgatgctatt tatgctaatc
    30901 cgctgctcgc gcacctgcct gcagtacaaa acaagcagat ttatgcgctg ggaaccgaga
    30961 cattccgcct ggattactac agcgccatgc aagtgctgga acgacttaag gcgctgtttt
    31021 aagcattaat tgtcggacgc tgtcacctgc ggcggcgtct ggcgaaaacg ccgcaactcc
    31081 accagcacca gcaacaacaa cacgccgata atcaacaaac caaaaccgct cgcacttgcg
    31141 gaagcaaccg gtgtcatcat cgctcccaaa ccgcccagca gcgccgcgcc tatggcatcg
    31201 cccgtcacgt tttgcgccgt ccacaaaccg ttaatccgcc ctaacatcgc ttccggggtt
    31261 tgcgtttgca gcattgtata ttgcagcaac gaactaaccg cgctcagcca gccgaacagt
    31321 gccagacaaa ccacgcctaa aatccacatc ggcatcaggc caaacagacc aatggcgagg
    31381 aacgccccca atgtggagag caacatcaac agacctggcc gtacgctatg cgccagtttt
    31441 ccactggtta acgcgccaat agccgcaccg agcgggattg ccgcatagag aaagccaatt
    31501 tgcgccgccg acatctgcca gttgtcagcc agcgccggat acaggacccg caccgcgctc
    31561 gccatcgtca ataaaccacc cagcaacgca atccctccca ccagcgggct ggcgagtaga
    31621 aaacgaaatc ctgccagtaa tgatttcaac ggatgctcac gcggctgcgg cggtggtggc
    31681 aacgccggaa ggcttaacaa cggtagcaag gtaataaacg tgcccgccgc cgccagcccg
    31741 tagttccagg ctacgcctcc ggtcgccagc agtaaaccac caatcatggg cgaaatcacc
    31801 gaccccagac gcacagtcaa catggtgatc gccccagcct gcattaggtt ttcacgtcct
    31861 accagtgcag gtgtcgccgc cagtagcgcc gtaacgccaa gtgatgcgaa aaaaccatcc
    31921 cataaaccaa gtaaatagat tgccagcaaa gacggctccg gcagcagcgc attcaggcac
    31981 agtccaatga agccaatgcc acaggtgccg cgcgccagca aaatcacttt tttgcgctca
    32041 tagcgatccg ccagcacacc accgatcatc agaccgacaa acatagcacc gccggtcagc
    32101 gtcaccgaaa gccccacctg ccaggtagaa tgtgtcatca tctggatctg caccggcacc
    32161 gcgacgccga gcaaacccag agacacaatt gagataaaac gagcgaggaa tactgcgcga
    32221 aacgccgggt gcgtttttaa caggctgagg ttaagcagcc aggattgttt attcattaca
    32281 atgccttgcc atcaaatatt ttaaatacct attcttaggc tgcacatgct aacatatcca
    32341 aataagatcg ataacgataa ttaatttcat tatcatggaa gttcgtatgt ctggttctgt
    32401 tgccgtgaca cgcgctattg ccgtgcccgg attgctgtta ttactgatta tcgcgacggc
    32461 attaagcctg ctcattggag caaaatcact ccccgcttcc gtagtgctgg aagccctctc
    32521 cggcacctgc cagagcgccg actgcaccat cgtgctcgac gcacgactgc cgcgcaccct
    32581 tgccggttta ctggcaggcg gtgcgcttgg ccttgccggg gcgttaatgc aaaccctcac
    32641 ccgaaaccca cttgccgacc ccggcttact tggcgtgaat gccggagcca gctttgccat
    32701 tgtgctgggt gcggcgctgt ttggttactc ttccgcgcag gaacaactga tgatggcctt
    32761 cgccggcgcg ctggtggcgt cattgattgt tgcctttacc ggcagccagg gcggcgggca
    32821 gttaagtccg gtgcgtttaa ccctggcagg cgtggcgctg gcggcggtgc tggaagggct
    32881 gaccagcggc atcgccctgc ttaatcctga cgtctacgat caactgcgtt tctggcaagc
    32941 cggttcgctg gatattcgca atctacatac cttaaaagtg gtgctgatcc cggtgcttac
    33001 cgccggagca accgcgctat tactgagccg cgcgctgaac agtttaagcc ttggcagtga
    33061 caccgcgacg gcgctgggca gtcgcgtagc gcgcacacag ttgattggtc tgctggcaat
    33121 taccgtgctt tgtggtagtg cgacggcggt agttggcccg attgccttta ttggcctgat
    33181 gatgccgcac atggcgcgct ggctggtagg tgccgatcat cgctggtcgc tgcccgtcac
    33241 gctactctct acccctgccc tgctgctgtt tgccgatatc attgggcggg tgattgttcc
    33301 cggcgaactg cgcgtttctg tagtcagtgc ctttattggt gcaccagtgc tgatcttcct
    33361 cgtacgacgt aaaacgcgag gtggcgcatg atttacgtct ctcgccgatt aattatcacc
    33421 tgtctactgt tgctaatagc ttgtgtaatg gcaggcgtgt ggggattacg cagcggtgcc
    33481 gtcacgctgg aaacctcgca ggtattcgcc gcgctgatgg gcgatgcgcc gcgcagtatg
    33541 acgatggtgg tcaccgaatg gcgtttacca cgcgtgctga tggcgctgtt gattggcgcg
    33601 gcgctgggcg tcagcggcgc gatttttcag tcgctgatgc gtaacccgct cggcagccct
    33661 gacgtaatgg gctttaacac cggggcgtgg agcggcgtgc tggtggcgat ggtgctgttt
    33721 ggtcaggacc tgacggctat cgccctggca gcaatggtgg gcggcattgt cacttcgctg
    33781 ttggtctggc tgctcgcctg gcgtaacggt atcgacacct ttcggttgat tattatcggt
    33841 atcggcgttc gcgccatgct ggtggccttt aatacctggc tgttgctgaa agcgtcttta
    33901 gaaacggcgc taacagcggg tttgtggaat gccggatcgc tcaatggcct gacgtgggca
    33961 aaaacctcac cttccgcacc catcattata ttaatgttaa ttgccgctgc cttgctggtg
    34021 cggcgaatgc gcttgctgga aatgggcgat gataccgcgt gtgcgctggg cgtcagcgtc
    34081 gaacgttcgc ggctgttaat gatgctggtc gccgtagtgc ttaccgcagc tgccactgcc
    34141 ctggcggggc caatttcctt tattgcttta gtcgcgtcac acattgcccg ccgcatcagt
    34201 ggcaccgctc gctgggggct gacccaggcg gcgctgtgcg gggcactttt actgctggcg
    34261 gccgatctct gcgcccaaca actgtttatg ccgtatcaac ttccggttgg cgtcgttacc
    34321 gtcagcctcg gcggtattta ccttatcgtc ttgttaattc aggagtctcg caaaaaatga
    34381 ccgaatcagt agcccgtttg cgtggcgaac agttaaccct cggatatggc aaatataccg
    34441 ttgcggaaaa tctgactgta gaaatacctg atggtcactt cacggcaatt atcgggccaa
    34501 atggctgcgg taaatccacg ttactgcgta ccttaagccg cctgatgacg cctgctcacg
    34561 ggcatgtctg gctggatggc gagcacattc aacattacgc cagtaaagag gttgcacgcc
    34621 ggattggtct gttggcgcaa aatgctacca caccgggcga tatcaccgtg caggagctgg
    34681 tggcgcgtgg gcgttacccg catcaaccgc tgtttacccg ctggcgcaaa gaagatgaag
    34741 acgcggtaac aaaagcgatg caggccacgg gaataactca tctggcggat caaagcgtgg
    34801 acaccctttc tggcggacaa cgccagcgag cgtggatcgc gatggtgctg gcccaggaaa
    34861 cggcaattat gctgctcgac gaacccacga cctggctgga tatcagtcat cagattgatt
    34921 tgctggaact gttaagcgaa ctgaaccgcg agaaaggcta taccctggcg gcggtgctgc
    34981 acgatcttaa tcaggcctgt cgttacgcca gccatttgat tgcattgcgg gaaggaaaaa
    35041 ttgttgctca gggagcgccg aaggagattg tcactgctga actgatcgag cgcatttatg
    35101 gtctgcgctg catgattatt gacgatccgg ttgccggaac gccgctcgtg gtgccgctcg
    35161 gacgaacgac accttcaacc gcgaagattt aaactaagtg gtctgccatt atggcatcct
    35221 gttttctgga tgccatcgca tgacgcaaca gcacaccacc acaagccacc atcccgccga
    35281 tcaacgcgga aaggatcacg ataatagcct tacccggacc gtcttttttc actggcaatg
    35341 acgggcttaa ctgatattta
//
LOCUS       DAZFFE010000037        34662 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFFE010000037 DAZFFE010000000
VERSION     DAZFFE010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.037"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(90..410)
                     /gene="chbB"
                     /locus_tag="ACHJ69_002038"
     CDS             complement(90..410)
                     /gene="chbB"
                     /locus_tag="ACHJ69_002038"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO5015027.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(709..1047)
                     /gene="osmE"
                     /locus_tag="ACHJ69_002039"
     CDS             complement(709..1047)
                     /gene="osmE"
                     /locus_tag="ACHJ69_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO5015028.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQATK"
     gene            1249..2076
                     /gene="nadE"
                     /locus_tag="ACHJ69_002040"
     CDS             1249..2076
                     /gene="nadE"
                     /locus_tag="ACHJ69_002040"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO5015029.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2306..3193
                     /gene="cho"
                     /locus_tag="ACHJ69_002041"
     CDS             2306..3193
                     /gene="cho"
                     /locus_tag="ACHJ69_002041"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO5015030.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLSEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHDDHALRLRQSLERLRVVCW
                     PWKGAVALKEQHPEMTQYHIIQNWLWLGAVNSLKEATTLIRAPAGFDHDGYKILCKPL
                     LSGNYEITELDPVNDQQAS"
     gene            complement(3153..3728)
                     /gene="ves"
                     /locus_tag="ACHJ69_002042"
     CDS             complement(3153..3728)
                     /gene="ves"
                     /locus_tag="ACHJ69_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO5015031.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASITSIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFSFAADLVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3931..4416)
                     /gene="spy"
                     /locus_tag="ACHJ69_002043"
     CDS             complement(3931..4416)
                     /gene="spy"
                     /locus_tag="ACHJ69_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO5015032.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHEDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4746..5714)
                     /gene="astE"
                     /locus_tag="ACHJ69_002044"
     CDS             complement(4746..5714)
                     /gene="astE"
                     /locus_tag="ACHJ69_002044"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO5015033.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQDPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMANDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5707..7050)
                     /gene="astB"
                     /locus_tag="ACHJ69_002045"
     CDS             complement(5707..7050)
                     /gene="astB"
                     /locus_tag="ACHJ69_002045"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO5015034.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHCFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGIQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPVVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRSRVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWRY
                     LNELLAADNPISSLKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDTLTQLLDLGSVYPFQREGGGNG"
     gene            complement(7047..8525)
                     /gene="astD"
                     /locus_tag="ACHJ69_002046"
     CDS             complement(7047..8525)
                     /gene="astD"
                     /locus_tag="ACHJ69_002046"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO5015035.1"
                     /translation="MTLWINGDWVTGQGALRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFGDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGDAVMRLWQQAGLPPGVLNLVQGGRATGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLFLKSGTQGDAFLARLVAVSQRLTPGTWDDEPQPFIGGLISEQAAQQVVTAW
                     QELEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVTGLPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8522..9556)
                     /gene="astA"
                     /locus_tag="ACHJ69_002047"
     CDS             complement(8522..9556)
                     /gene="astA"
                     /locus_tag="ACHJ69_002047"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO5015036.1"
                     /translation="MMVIRPVERSDVSALMLLASKTGGGLTSLPANEATLSVRIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPEWRKEGNGYLLSKSRFMFMAAFRDKFNDKAV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYCNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9553..10773)
                     /gene="astC"
                     /locus_tag="ACHJ69_002048"
     CDS             complement(9553..10773)
                     /gene="astC"
                     /locus_tag="ACHJ69_002048"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO5015037.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSDIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASAQIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNSINHHYSLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAVKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FASACEHFVSRGSS"
     gene            11219..12025
                     /gene="xthA"
                     /locus_tag="ACHJ69_002049"
     CDS             11219..12025
                     /gene="xthA"
                     /locus_tag="ACHJ69_002049"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO5015038.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAEYCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12192..12902
                     /gene="ydjX"
                     /locus_tag="ACHJ69_002050"
     CDS             12192..12902
                     /gene="ydjX"
                     /locus_tag="ACHJ69_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO5015039.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLSHPKNEG"
     gene            12907..13584
                     /gene="ydjY"
                     /locus_tag="ACHJ69_002051"
     CDS             12907..13584
                     /gene="ydjY"
                     /locus_tag="ACHJ69_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO5015040.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKASSFDEVIVDSNGKKLNMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            13598..14305
                     /gene="ydjZ"
                     /locus_tag="ACHJ69_002052"
     CDS             13598..14305
                     /gene="ydjZ"
                     /locus_tag="ACHJ69_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO5015041.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAVLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            14305..14853
                     /gene="ynjA"
                     /locus_tag="ACHJ69_002053"
     CDS             14305..14853
                     /gene="ynjA"
                     /locus_tag="ACHJ69_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO5015042.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRARLATHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            14863..16029
                     /gene="ynjB"
                     /locus_tag="ACHJ69_002054"
     CDS             14863..16029
                     /gene="ynjB"
                     /locus_tag="ACHJ69_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5015043.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPTALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKANTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFITIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            16002..17537
                     /gene="ynjC"
                     /locus_tag="ACHJ69_002055"
     CDS             16002..17537
                     /gene="ynjC"
                     /locus_tag="ACHJ69_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO5015044.1"
                     /translation="MATPLRYALIFLLWAMMAVIYAPLIPAALTLISLALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQLIVLDSLGYSRWQCLNWLLLPSVASALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLILLLAAYVLLGYLLWRGWRRTIPRVDGVRKP
                     ATPLLPGNTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVAAFIALLLLL
                     LWQEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPALIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLSLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            17537..18190
                     /gene="ynjD"
                     /locus_tag="ACHJ69_002056"
     CDS             17537..18190
                     /gene="ynjD"
                     /locus_tag="ACHJ69_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO5015045.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFSVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRHWVFSEIRAMAIPVVQVTHDLQDVPPDSPILDMAQWSENYNKLR"
     gene            18257..19564
                     /gene="ynjE"
                     /locus_tag="ACHJ69_002057"
     CDS             18257..19564
                     /gene="ynjE"
                     /locus_tag="ACHJ69_002057"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO5015046.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNEWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKFYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPIATGERGPDSSK"
     gene            complement(19573..20193)
                     /gene="ynjF"
                     /locus_tag="ACHJ69_002058"
     CDS             complement(19573..20193)
                     /gene="ynjF"
                     /locus_tag="ACHJ69_002058"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO5015047.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVAILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALVGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            20280..20687
                     /gene="nudG"
                     /locus_tag="ACHJ69_002059"
     CDS             20280..20687
                     /gene="nudG"
                     /locus_tag="ACHJ69_002059"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO5015048.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAHSDQAGLWEFAGGKVEPDES
                     QRQALVRELNEELGIEATVGDYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALRYPLAPADIPLLEAFMASRAARPAD"
     gene            complement(20653..20925)
                     /gene="ynjH"
                     /locus_tag="ACHJ69_002060"
     CDS             complement(20653..20925)
                     /gene="ynjH"
                     /locus_tag="ACHJ69_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO5015049.1"
                     /translation="MSRALIAVVLAFPLITLANPHYHPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            21161..22504
                     /gene="gdhA"
                     /locus_tag="ACHJ69_002061"
     CDS             21161..22504
                     /gene="gdhA"
                     /locus_tag="ACHJ69_002061"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO5015050.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVTDYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            complement(22581..24536)
                     /gene="topB"
                     /locus_tag="ACHJ69_002062"
     CDS             complement(22581..24536)
                     /gene="topB"
                     /locus_tag="ACHJ69_002062"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO5015051.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTSVRQFRGIMAPGGRE
                     GKKKDSPRKRAPKKSPPSEEAGNGVIT"
     gene            complement(24541..25584)
                     /gene="selD"
                     /locus_tag="ACHJ69_002063"
     CDS             complement(24541..25584)
                     /gene="selD"
                     /locus_tag="ACHJ69_002063"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO5015052.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(25701..26252)
                     /gene="ydjA"
                     /locus_tag="ACHJ69_002064"
     CDS             complement(25701..26252)
                     /gene="ydjA"
                     /locus_tag="ACHJ69_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO5015053.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDNKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            26413..28269
                     /gene="sppA"
                     /locus_tag="ACHJ69_002065"
     CDS             26413..28269
                     /gene="sppA"
                     /locus_tag="ACHJ69_002065"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO5015054.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSSAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITKALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            28436..29452
                     /gene="ansA"
                     /locus_tag="ACHJ69_002066"
     CDS             28436..29452
                     /gene="ansA"
                     /locus_tag="ACHJ69_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO5015055.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            29463..30104
                     /gene="pncA"
                     /locus_tag="ACHJ69_002067"
     CDS             29463..30104
                     /gene="pncA"
                     /locus_tag="ACHJ69_002067"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO5015056.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLKQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRAHVINELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(30197..31555)
                     /gene="ydjE"
                     /locus_tag="ACHJ69_002068"
     CDS             complement(30197..31555)
                     /gene="ydjE"
                     /locus_tag="ACHJ69_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5015057.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGRIAGAESQLR
                     EVEQQIEREKSIRLPPLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(31673..32431)
                     /gene="ydjF"
                     /locus_tag="ACHJ69_002069"
     CDS             complement(31673..32431)
                     /gene="ydjF"
                     /locus_tag="ACHJ69_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5015058.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(32568..33548)
                     /gene="ydjG"
                     /locus_tag="ACHJ69_002070"
     CDS             complement(32568..33548)
                     /gene="ydjG"
                     /locus_tag="ACHJ69_002070"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFO5015059.1"
                     /translation="MKKIPLGTADITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPKSIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNALKAE
                     GKIRAIGAANVDADHIREYLQHGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            complement(33558..34505)
                     /gene="ydjH"
                     /locus_tag="ACHJ69_002071"
     CDS             complement(33558..34505)
                     /gene="ydjH"
                     /locus_tag="ACHJ69_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO5015060.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGRALTEIFTQA
                     KARQMIICADMIKPRLNETLNDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSIGATTGVKNRKLVEQLLEEYEG"
     gene            complement(34510..>34662)
                     /locus_tag="ACHJ69_002072"
     CDS             complement(34510..>34662)
                     /locus_tag="ACHJ69_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO5015061.1"
                     /translation="IHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALDKIKLFG
                     SDGKAE"
BASE COUNT         8408 a         9188 c         8589 g         8477 t
ORIGIN      
        1 ctcatagaaa aatacccttt aaaaccgcaa tttaaatatt gcggtattga tttatgaaat
       61 aactctttga cgggaaaatt taaaataaat taatttgctg cggctttttt aatcgctgca
      121 accgcagcct taagcacgcc taaaccatcg actttgccat aaagcagcga gtcaattact
      181 tcaaccggtt tgttgggtaa caaacgctgg atttcgggca acatataagc aatctgcggc
      241 cctaataaca cgacatcggc attctgacct ttttcaccag ccagtgtttc cggaaatgct
      301 tcaataatga ccggaacttc atatttttct gcctgtgcgc gcatttttga taccagtaaa
      361 gaggtagaca tgcccgcaga acaaaacaga taaatgtgtt tcttttccat aaaactgccc
      421 tcatcgacga ttatctgtca gccagacact ccgcaagcct taacctgctt ccatgctctg
      481 ggtaacttgc gaaaccaaca tgactttttt tgtggctgaa aggagtatac ggtataggcc
      541 tgagcaatag tatttctttg accttataaa ttgtctctcc ttgacctgtg gttatgaccc
      601 tcttcacatt tcgggcaaat aattcgcgaa gataattaag aacacaggcc aaaaaaaacc
      661 ggcgcaatgg ccggtttccg ttgttactca agctttcagg cgaagtgatt acttcgtagc
      721 ctgtggatca gtgtcgtatt cagcacaggt ctgataaccg gagttgatga catgtccggt
      781 atcatctaac gcgacaaagt aggtttctgc tttaccatca cgttgaccca ggatgtaggt
      841 ctggcaagta ccacgagcat ggatcatgct cacttcagac gaaggtttac ccgcaatttg
      901 tgcaacctgc gcccggctca tgcctttttt gacgtctttc accacaggct gtacaaactg
      961 gtctttggta cgatcataag ccgtacaacc cgccagcatg gttaataccg ccgctgcact
     1021 cagaattcct gccatattct tgttcatatt ccgtcctctt gtttatcagc gtgttagata
     1081 agcctggaat acattgggcg ctttttcaag cccgtgaacg aaacggctcc gctttcagag
     1141 gattcctgta tgacgtttta accaccattc agcccgctgt cgcttgtcgt ttcagtagca
     1201 acgggttagc tttaaggaag ttttgtcttt tctgtctgga ggggttcaat gacattgcaa
     1261 caacaaataa taaaggcgct gggcgcaaaa ccgcagatta atgctgaaga ggaaattcgt
     1321 cgtagtatcg attttctgaa aagctacctg caaacttatc cgttcattaa atcactggtg
     1381 ctcgggatca gcggcggtca ggattccacg cttgccggta agctgtgcca gatggcgatt
     1441 aatgagctgc gccaggaaac cggcaacgaa tcactgcaat ttattgccgt acgcctgccc
     1501 tatggtgttc aggccgacga acaagattgc caggatgcca tcgcctttat tcaaccggat
     1561 cgcgtattaa ccgttaatat caagggcgcg gtactcgcca gcgagcaggc gctgcgcgaa
     1621 gcgggcattg aactgagcga ttttgtccgt ggcaatgaaa aagcgcgtga gcggatgaaa
     1681 gcacaatata gcattgcggg tatgaccagc ggtgtcgtgg tgggcaccga tcatgcagca
     1741 gaagccatta ccggattctt cactaaatat ggtgacggcg gtacggacat taatccgctg
     1801 tatcgtctca acaaacgtca gggtaaacag ttactggcgg cattaggttg cccggaacac
     1861 ctttataaga aagcgccaac ggccgatctt gaagatgatc gcccttctct accagatgaa
     1921 gtggcactcg gcgtgaccta tgacaatatc gacgactatc tggaagggaa aaacgtacct
     1981 gaacaggtcg ccagaacaat agagaactgg tatctgaaaa ccgaacataa acgccgtccg
     2041 ccaattaccg ttttcgatga tttctggaaa aagtaataat ttgcacatat tggtttgtgc
     2101 gaaaaagagt actttgttca cgccggatgc ggcgtgaacg ccttacccga cctacaaaac
     2161 tatgcaaatt caatacattg caggagtcga ataggcctga caggcgtagc acgtcagacg
     2221 gtataacctt tgtcatcgcc ccgcctcttt tttaatcgct ccccgcctgt tacactggat
     2281 agataaccag cactcggagt caacagtggt acggcgttta acttctccgc ggctcgaatt
     2341 tgaagctgcg gcaatttatg aatatcccga acatttacgt tcattcctta atgacttacc
     2401 cacccgacca ggggtgtatc tgtttcatgg cgaaagtgac accatgccgc tctatatcgg
     2461 caaaagcatt aacatccgca gccgcgtcct ttctcattta cgtaccccgg atgaagccgc
     2521 catgctacgg caatcccgac gcatcagctg gatatgtacc gccggtgaaa tcggcgctct
     2581 gctccttgaa gcgcgattaa tcaaagaaca acagccactg tttaataaac gcttgcggcg
     2641 caatcgccaa ctctgtgccc tgcaattaag tgaaaagcgc gtcgatgtgg tgtatgccaa
     2701 agaggtggat ttttcacgag cccccaacct gtttggcctg tttgccaata ggcgcgcagc
     2761 tttgcaagca ttgcagacca tcgctgatga acaaaaactt tgttatggcc tgttgggact
     2821 ggagccgtta agtcgcggtc gtgcatgttt tcgttcagcg ctaaaacgtt gcgccggagc
     2881 atgctgcggt aaagagagcc atgacgatca tgcgctacgt ttgcgccagt ctctggaacg
     2941 tttacgagtc gtttgttggc cgtggaaagg tgcggtagcg ctgaaagaac agcacccgga
     3001 aatgactcaa tatcatatta ttcaaaactg gctgtggctg ggggcggtta attcgctgaa
     3061 agaagcgaca acgttaattc gggcacccgc cgggtttgat cacgacggtt ataaaattct
     3121 ttgtaagccg ctgctttccg gtaactatga aattactgaa cttgatccgg tgaatgacca
     3181 gcaagccagt tgatttcact gaacaacagt ttaccttgcg gttgcaaaag ccgcagggtg
     3241 tgcctgccat cgaaccagca cgcgccctgg tcggtagtga gcagtttgtc gcccaattgc
     3301 cacgccccgt ttattacaaa caccaccccg ccgcgtgaac caaaggtagt aaacgtacgt
     3361 tcggcaattc tgactttcgc cttacagaca tccaggcgtg tcatgatgtt gaaatccatc
     3421 gacatctgcc ctgccgtcag tttcgctttt accaccaggt ccgctgcaaa ggaaaaaggt
     3481 tgtaacggtt ttaaggtatg gttaaagcgg tctgcgcttt caaggaacat ctcgccgcct
     3541 tccagcaacg tcactatcct ttccataccg ggaaacaaag aaaactcccc attcgccgcg
     3601 atggatgtaa tgctggcacg ccagtaaaaa tcacgttttg ccggtgggaa agtgcaaatt
     3661 tcgcgcgttt caccggcagc gtttcgccac agattcaccg acattttacg catatcaaag
     3721 tattccatac ccgctcccgg cccgttcttt tcattgttat cgttcaggta accgtggatg
     3781 gttatcggca actcgggtga tttacgttag tggcgatcag gctttttttg catctgtttg
     3841 agaagagaaa agaaaaccgc cgatcctgtc caccgcatta ctgcaaggta gtggacaaga
     3901 ccggcggtct tacgtttttt ggctgaaaga ttattcagca gttgcaggca ttttaccttt
     3961 tgccgctgga cgttctgtca gacgcttctc aaaattagca ttaaattgct ttttctgttc
     4021 cggcgtcagg atgttgtaaa tcttgttctg ggtttccatg tgcgccagca tgttagcttt
     4081 gcgctgttct tccatttttg cgatctgcgc ttcagctttt gctttatcga aggtatcgct
     4141 ggcaatgatg tcatgcattg cgcggcgttc ttccagcggc ggacgtttca tctggtcacg
     4201 ctggcctttc atgatttcgc ggatctgctg tttctgcgcg tcggtcaggt tcaggtcttt
     4261 gaacatcatg tcctcatgcg gaccgaactt gcctttatgg tgcatcatcg gcttcgcgtc
     4321 agccggtgct gcggtagtgg tgtctgcggc atgggccagg ttagccgcgc caagagccag
     4381 ggtagaggca acaaacagtg cagttaattt acgcatattc tatatccttc ctttcagtta
     4441 tttattacgg ctttcttaag tagcgtgccg tgttgacgag attaacttta ctgactttag
     4501 cgtcaattaa tcagagcaac ggtaaaacaa tgaaagtgta aaaaacactt ttgcgccaat
     4561 tatggagaaa aaaagaaaat ttgatggaga gtgatgagag aatattacaa cacgatgatt
     4621 ttgcagagat tatgaagaac tataccggat gactggtgat aaataaagca aataaccagg
     4681 attaatctgt attaatttat aagaaagcaa cttaataccc gcagaatgat ttctgcgggt
     4741 aagtattagc ttattttttc gagcattaat cccgcacgta atcccaacgc taccaacgga
     4801 ttagggaata acacatactc tacatcatgg gttacggtaa aacgttcctc cccgtcctgc
     4861 gccagcaatg ttcctttctc aaacggcata aaattcagcg tgtcatttgc catatgcatt
     4921 tcgaaggacg gcgagtggcg agtaatttgc gaaaccaccc gataacggag cggcggtgtt
     4981 ctcacgatac cgacactctc accagatagc agcgcagcaa ttgcgctggc agttactgca
     5041 aactggcgaa gatcgttttg cccaaagggc aacgctttgc caagttccag cgtacaggcc
     5101 agcgcgccaa aatgtctggc gctgaaatgg gtaaacgtac caccagggtc ctgatggaac
     5161 accagcgcct ccagccccgc cgcacccagc cacgtcagaa atttctcgtc ccagggaatg
     5221 tcgcgttgcg gtaatacacc gaactgcgga tgcaaggagc cacgaattgc ggtatgtaga
     5281 tcaaggtgcc agcgcacaga ttctttgccc tggtcataaa aatcttccag gcactgctcc
     5341 agttcgcgcg cccgacaggt ttccccgctt tcagcaaaca gttgccaacg accgccaaac
     5401 attcgattca tatcgctatg gcaataacgt ttcccttgct tcagcgcagg aggattcccg
     5461 aggatcacca gtaaccgcca acgtaacggg atctcaccgt gagatatcgc gccaagcaac
     5521 gcgtccagca tttccacagg agccgtctca ttaccgtgga tccccgctga aatcaccagc
     5581 gcgccttgcg gtggcgttaa tggcgtcagt tccagcacac catcgcccag ccagcgccag
     5641 cgaacgccgt tgatttctcg ctcggtgata accggttttt tacccgttaa ggttagagca
     5701 agaaaattat ccattgcccc ctccctcgcg ctggaatgga taaaccgaac caagatccaa
     5761 taattgagtc agtgtatcca gcgcttccct tccttcgcgc agcaattgtg gatcggcaag
     5821 atccgccgca gtaagacgat cgcggtagta acgatccacc cagtcattga gcgcattaaa
     5881 cagcgtatcg ttcatcatca ccgccggatt caccgcccgg cgttcttctt ctgtcaacac
     5941 cacgcgcaac cgcaggcacg ccgggccacc gccattcgcc atgctttcac gtaaatcaaa
     6001 aacttttagt gagcttattg ggttatcggc agcaagcaat tcattgagat aacgccatac
     6061 tccagcatgt tcccgacatt cctgaggcag caccaacatc atggaaccat catcgcggct
     6121 cagtaactgg ctgttaaaca gatacgtaga caccgcatcc gacacggaaa cctgagtcgc
     6181 cggaacttct atcgccataa aaccattgac ccgcgaacgc aggtttgcca gtaactgtga
     6241 ctggcgagcg aacgcctgtt gatggcaaaa cagcacctgg cggttactca cggcaatcac
     6301 gtcattatga aaaacgccct ggtcgataac gaccgggttt tgctgggcga aaatcacctg
     6361 ttggggattc acctgattca accttgccac cgcctcgctg gcttcgcgag tctgccgcgc
     6421 cggataacgg gaaggccggg tatcattgcc ctcttctcgc ccgtagacaa aaagttgaat
     6481 acccggttca ccgtaatgac cgccgagacg attgtggttt gccgctccct catcaccgag
     6541 caaggctacc tgcggcaacg ccgaatggac actaaatttc tcttcgtcgt taaaaatcgc
     6601 ttttaacaac gattcggtga cgggggcttc cagcgaacgg tgaaatttat tgttcaggtt
     6661 ggcaacggtg agatgcactt tgccatccag cgtatcggca gatggcgcga tcgttgccgc
     6721 attggctacc cacattggcg aagcggagct gacgctggaa agccagtgcg gtgcctggcg
     6781 ggcaaccttt tccagcactt gttcatcgct accactgaat cccaactgac gtaaaaccgg
     6841 tataaacgga cgctcgtgcg gcgggatcac ggcctggggg aatcccgcat cggcaagggt
     6901 tttcattttc agtaagccct gtttcgccgc cagtcgcggg ttagagacct gaaaacaatg
     6961 acgggtagag gcttcattac caaacgacag gcccgcgtaa tgatgcgtca gccctaccag
     7021 cccgtcgaaa ttgacttccc aggcgttcat cgcaccacct catcggaaaa atccagccca
     7081 gggttaagcg tagcgggcaa tgttaacgag tccgactcca ggctcgccat cggccaggca
     7141 caataatctg cggcatacca ggcgctgggg cgatggttac cggaagcgcc aatgccgccg
     7201 aatggcgcgg tactggcagc gccggtaagc ggtttgttcc agttaacaat ccccgcccgc
     7261 gcctccagca acagttgatc gaatttttcc cgctcggggg aaaccagacc gcaagagagg
     7321 ccaaagcgag tgttattcgc cattcgaatc gcttcatcga aagtatcata acgccagacg
     7381 cgcaataacg gtccgaacac ttcttcatcc ggcaagccag taacgcctgt catttcaatg
     7441 atccccggcg tcagcaacga tgtccctgct tgtaataagc gcggcgcaag cagggtccgt
     7501 ccgcccatcg cctccagttc ctgccaggca gtaaccacct gctgtgcggc ctgctcagaa
     7561 atcagcccac caataaatgg ctgcggttcg tcatcccaag tgcccggcgt taaccgctgg
     7621 ctgacggcaa ccaggcgagc aagaaatgca tcgccctgcg ttccactttt cagaaataaa
     7681 cggcgggcgc aggtgcagcg ttgcccggca gtgacaaacg ccgactgaat ggtcagatgg
     7741 acagcggcat cgatatccgc cacctcatcg ataattagcg ggttattacc gcccatctca
     7801 agggcgagaa ttttctccgg ctgaccggaa agctggcgat gcagctggta gccggtattg
     7861 gcgctgccgg taaacagcaa gccgtcgaga tcctccagcg cactcagcgc ctgacccgtt
     7921 gcacgcccgc cctgcaccag gttcagcacg cccggcggca agccagcctg ctgccataaa
     7981 cgcattaccg cgtcgccact ccacggtgtc agttcgctgg gtttaaagat aatggtgtta
     8041 cctgccagca atgccggaac gatatgtccg ttcggcaaat gaccagggaa attatacggc
     8101 ccaaacaccg ccagcacgcc gtgcgggcga tgtcgcaggc tcgccgcgcc gtccggcatt
     8161 tcgctacgct gctcaccggt acgaacgtga tacgccttaa ttgatatcgc gattttattg
     8221 atcatcgccg tcacttcggt tgccgcttcc cagcgcggct tgccggtttc tctggcaata
     8281 atcgcggtta attcggcttt attgctttcc agcaatccgg caaagcgttc aactcttacc
     8341 tgacgatcgc caaatgagag ccgagcccag cgcggaaacg ccgcacgggc agcccgacaa
     8401 gcctgcccga cctgagcggc atcggcatca ttgccttgcc ataacacctc gcccgatacc
     8461 ggattacgct tcacacgcaa tgcgccctgg cccgttaccc agtcaccgtt gatccataaa
     8521 gtcatgctgt tttctcctct gcgcacaggc gcaccagacg aacgcgatcc cctgcgtggc
     8581 atttgagggc atccagttgt gcggcggtta aaatcaaacg ctcggtcgct ggatcggtac
     8641 gcaccagcac cacgcggaaa tggtgataat tttcattggc gaccaggcag gccggaaaat
     8701 cgccctgcgc aggctgccct tcggccactt ccaccagccg acttttacgg atggcgcgca
     8761 cgcggtcgat atcacattca agtgtcggcc cgccgtcaaa gatgtcgata tagttacagt
     8821 agcgaaaacc ttctttctcc agcaccgcac gggcaggcgc ggtttgcgga tgtacctgac
     8881 cgatgacgtc ctgggcttcc tgggataaaa agtgggtata gatcggatgt ttcggcatca
     8941 gttctgcaat aaatgccttt tgcccggtgc cgcagagaaa atcagcgcgg ctaaaatcca
     9001 tcgaaaagaa gcgtttaccg aggctttgcc agaacggtga atagccgtgt tcgtcaatca
     9061 ccccgcgcat ttcggcgacc gctttgtcat taaacttgtc gcgaaaagcc gccataaaca
     9121 taaagcgcga tttcgacagc aaatagccgt tgccctcttt gcgccactcc gggtcgagaa
     9181 acagcgtgca cagctcgctg ctgccggtgt gatcgttact gagaaacagc gtcggcaatg
     9241 cgttatagac attcagctct ttcgaagcgt gaaccagcgt gccgacacga tagttgtacc
     9301 agggatcgtt cagcccaacc gccacctcaa tggcgcaaat ccccgccaca gtgcctgtct
     9361 cactatcttc cagtacaaac acataaccct gctcactttt gggcagttcg ccttgccagg
     9421 ttttgattgc cctttcaata cgcaccgaaa gcgtggcttc attggcggga agcgacgtca
     9481 ggccgccgcc cgttttgctg gcaagcagca tcagcgccga gacatctgag cgctcaacgg
     9541 gacgaatgac catcatgatg aacctcggct aacaaagtgt tcgcaagcag atgcaaagcg
     9601 atccagtccg gtcgtcacct cttcttcgct gacattgagc gcaggcgcaa agcgcaccac
     9661 gttgccaccc gcaatcagca ccatcacgcc tgctttcacc gcttcctgag agatctgttt
     9721 cgcttgcccg gcgtaatcgg cattcagtac acagccaatc aaaagaccta agccgcgaac
     9781 ttcactgaac aaactgtagt ggtgattaat ggaatttaga cgctcaacaa accagtcgtg
     9841 acgctgttta accccgttaa gcatctctgg cgtgttgatg agatccagca ctttgcctgc
     9901 caccgccgag gccagcggat tgccgccata ggtggtgccg tgagtgccaa cggtcatcac
     9961 gctggcgcac tcttcggttg ccaacaacgc accaaccggg aaaccgccgc ccagcgcttt
    10021 ggcggtagtt aacagatcag gcgtcacgcc gtagtgcata taggcataca gttccccggt
    10081 gcgcccgaca ccggtttgta cttcatcaaa gatcagcaac gcattgtggc ggtcacacaa
    10141 ttcacgcaga ccttgtaaaa atgcgttgct ggctggcacc acaccgcctt ccccctggat
    10201 gggttcgaca atcaccgcac aggtggcgtc gtcaatctgc gcactggcag agttaatatc
    10261 gttatatgca gcatgacgaa tatccggcgg cagtggcgca aaatcctgtg aataggctgg
    10321 ctgcccaccc gcactgacag taaacagcgt gcgaccatga aacgcatttt tgaacgccac
    10381 gatgtcgctc ttatggctgc cgtagcggtc gtgagcgaat ttacgcgcca gttttagcgc
    10441 cgcttcgttg gcttccgcac cggagttaca aaagaagacg cgatcggcaa acgtggcgtc
    10501 gatcaatttt ttcgccagtc gcagtaccgg ctcgttggtg taaccgttgc cggtatgcca
    10561 gaacttactc gcctgttcgt tcagcgcctc acgcagttcc ggatgcgcat ggcccagcgc
    10621 gttcaccgca atgccacccg cgaagtcgat atactctttc ccctgctgat cccacaagcg
    10681 cgaaccttcg ccacgtaccg gtataaacgg tgccggagcg taaacaggta tcatccattc
    10741 atcaaagttt tcacgcgtaa ttggctgaga catagcgacc tctacagtaa ataattcgtt
    10801 attcatatgt taataataag taatgtttgc gctgtaaatg tagattgcag ggttcgtgcc
    10861 agccagtgat aaaagtgcat aaacggcgga ggctaacttg aaatcaagca gttataacca
    10921 catcatatgc atttaaagtg catataaagt gaatacgttt gcgatgtggg tgaataaaaa
    10981 gaataaaaaa cgcaatgtta tgcagaagta aaatataatt ctggaattgt gatcattgac
    11041 gaaatttact ggaaattact gcgccattct gacgcaacgc gcaccaaaag cgggcatttt
    11101 ttgcgccatc gttgacatca ttaacaacca tcgatcaaat cacttaacaa caggcggtaa
    11161 gcaaagcgaa attctgctac catccacgca ctctttatct gaataaatgg cagcgactat
    11221 gaaatttgtc tcttttaata tcaacggcct gcgcgccaga cctcaccagc ttgaagccat
    11281 cgtcgaaaag caccaaccgg atgtgattgg cctgcaggag acaaaagttc atgacgatat
    11341 gtttccgctc gaagaggtgg cgaagctcgg ctacaacgtg ttttatcacg ggcagaaagg
    11401 ccattatggc gtggcgctgc tgaccaaaga gacgccgatt gccgtgcgtc gcggctttcc
    11461 tggtgacgac gaagaggcgc aacggcggat tattatggcg gaaatcccct cgccgctggg
    11521 caatgtcacc gtgatcaacg gttacttccc gcagggtgaa agccgcgacc atccgataaa
    11581 attcccggca aaggcgcagt tttatcagaa tctgcaaaac tacctggaaa ccgaactcaa
    11641 gcgtgaaaat ccggtgctga ttatgggcga tatgaatatt agtcctggcg acctggatat
    11701 tggcattggc gaagagaacc gtaagcgctg gctgcgtacc ggaaaatgtt ctttccttcc
    11761 ggaggaacgc gaatggatgg agaggctgat gagctggggg ttggtcgata ccttccgcca
    11821 tgcgaatccg caaacagcag atcgtttctc atggtttgat taccgctcaa aaggttttga
    11881 cgataatcgc ggtctgcgca tcgacctgct gctcgccagc caaccgctgg cagaatattg
    11941 cgtagaaacc ggcatcgact atgaaattcg cagcatggaa aaaccgtccg atcacgcccc
    12001 cgtctgggcg accttccgcc gctaatttag ctgatctcct ggctcaaact gggtcaggag
    12061 aattaacctt gagaaaaatc aacaaactgt cagtaatgat ttgttgcctg ccgtcctttg
    12121 ttataccgtc actgcgtttt tagttgtctg accacttctc tattatcaag tttgatatag
    12181 gaaactccac gatgaacgct gagcgtaaat ttctttttgc ctgtcttatt tttgcgctgg
    12241 tcatttacgc tatccacgct ttcggtttat tcgatctgct caccgattta ccccacttac
    12301 agacactcat tcgccagagc ggattgttcg gctatagcct ctatattctg ttattcatca
    12361 ttgccaccct ctttctgtta ccaggaagca tactggtgat cgcgggagga atagtttttg
    12421 gcccgctctt agggacacta ctatcattaa ttgccgccac gctggcctcc tcgtgctcat
    12481 tcctgctggc gcgctggctg ggacgtgatt tactgctgaa atacgttggc catagccata
    12541 ccttccaggc cattgaaaaa ggcattgcgc gtaacggtat tgattttctt attctgaccc
    12601 gcttaatccc gttgtttcct tacaatattc aaaattacgc ttacggatta accacaatcg
    12661 ccttctggcc ttataccctt atttcggcac tcacgaccct gcccggtatt gttatttata
    12721 ccgtgatggc aagcgatctc gccaatgaag gcattacgct gcgctttatt ttacaactct
    12781 gtctggcggg cctggcgctg tttattctcg tccagctcgc aaaactctac gcccgacaca
    12841 aacatgtgga tctgtctgct tctcgccgca gcccactttc tcacccaaaa aatgaaggat
    12901 agaacgatgt tgcaacatta ttcagtgtca tggaaaaaag gactggctgc actctgctta
    12961 ctggctgttg ctgggcttag cggctgcgat caaaaagaga atgcggctgc aaaagtggaa
    13021 tacgacggac tttcgaacag ccaaccgctg cgtgtcgatg ccaataacca tacggtaacc
    13081 atgctggtgc aaattaatgg tcgtttcctc accgacgaca ctcgtcacgg tattgtgttt
    13141 aaagatggct ccaacggaca taaatcgctg tttatgggtt atgcgacccc gaaagcattt
    13201 tatgaagccc tgaaagaggc aggtggtacg ccgggcgaaa acatgacgat ggataataaa
    13261 gaaacgactc atgtcacagg cagcaaactg gatatttcgg tcaactggca aggggcggca
    13321 aaagcgtctt ccttcgatga agtgattgtt gacagtaatg gcaagaaact gaacatgcgc
    13381 tttggcggta atttaacggc agcagaagag aagaaaacgg gttgcctggt gtgtctggat
    13441 agctgcccgg tcggcatcgt cagcaatgca acatacactt atggtgcggt ggaaaaacgc
    13501 ggcgaagtga aattcaaagg caatgcctca gttctcccgg cggataacac gctggcaacg
    13561 gttaccttta aaatcaccga ataaagcagg ataacggatg atgaagatgc aatcgcgtaa
    13621 aatctggtac taccgtataa ccctcatcat cctgttgttc gctgtgctgc tggcatgggc
    13681 gctgcttccc ggcgtccatg agtttatcaa tcgcagcgtc gcggcgtttg ccgccgtgga
    13741 ccaacagggt atagaacgct ttattcagtc ttacggcgca ctagcggcag ttgtctcgtt
    13801 cttgttgatg attttacagg ccattgctgc accgctacct gcgtttttga tcacctttgc
    13861 caatgcgtcg ctgtttggtg cgttctgggg cggcttactg tcgtggacca gttcgatggc
    13921 cggcgcggca ctgtgctttt ttatcgccag agtgatgggc cgcgaagtgg tggaaaaatt
    13981 aaccggcaaa accgtacttg acagtatgga cggctttttc actcgttacg gcaaacacac
    14041 catcctggta tgtcggttat tgccttttgt ccctttcgat ccaatcagct atgctgccgg
    14101 tttgacttca atacgttttc gctcgttttt tatcgccacc gggcttggtc aattaccggc
    14161 gactattgtt tattcctggg cgggcagcat gttaacaggc ggtactttct ggtttgtcac
    14221 cggactgttt attctgtttg ccctgaccgt ggtgattttt atggcgaaga aaatatggct
    14281 tgagcgccag aagaggaatg cctgatgggt ttaccgccgc ttagcaaaat tcctttcatt
    14341 ttacgtccgc aggcgtggct gcatcgtcgc cattacggcg aggtgctaag ccccattcgc
    14401 tggtgggggc ggatcccgtt tatcttttat ctggtgtcga tgtttgtcgg ctggctggag
    14461 cgcaaacgct caccgctcga tccggtagta cgatcgcttg tcagcgcgcg cattgcgcaa
    14521 atgtgcctgt gtgagttttg cgtggatatc accagtatga aagtcgccga gcgtaccggc
    14581 agcaccgata aactgctggc agtggctgac tggcggcaaa gcccgctctt tagcgatgaa
    14641 gaacggctgg cgctggagta cgccgaagcc gcaagcgtaa cgccgccaac ggtcgatgat
    14701 gccctgcgcg cccgactggc tacgcatttt gacgctcagg cgctcaccga actgacggca
    14761 ttgataggcc tgcaaaatct gtcagcccgt tttaattctg ccatggacat tcccgctcag
    14821 gggctgtgcc gtattcctga aaaacgttct taaggagaga agatgcgcca ttgtgggtgg
    14881 ttgctgggat tgttatcgct gttttctttg gcaacacatg ccagtgactg gcaggaaata
    14941 aaaaatgagg ccaaaggaca aaccgtctgg tttaacgcct ggggcggcga taccgcaatt
    15001 aaccgctatc tcgactgggt tagcggcgag atgaaaaccc attacgctat aaacctgaag
    15061 attgttcgtc tcgcggatgc cgcagacgcg gtgaagcgca ttcagaccga agctgctgcc
    15121 ggacgcaaaa caggcggttc ggtggatctg ctctgggtga acggcgaaaa cttccgcacc
    15181 ttaaaagagg ccaatttatt acaaacgggc tgggcggaga ctctgcccaa ctggcgctat
    15241 gtcgacacac agcttccggt gcgggaagat ttttcagtgc ctacagaagg ggctgaatcg
    15301 ccctggggcg gcgcacaact gacgtttatc gcccgccgcg atgttacgcc acagccacca
    15361 caaacgccgc aagccttact ggagtttgct aaagccaatc ccggcacggt tacctatccg
    15421 cgcccaccgg actttaccgg tacggcgttt cttgaacagt tgctgattat gctaacgcca
    15481 gatcccacag cattaaaaga agcgccggac gatgcgactt tcgcccgcgt cactgcgccc
    15541 ttgtggcaat atcttgatgc actgcatccg tatttgtggc gcgaaggaaa ggatttcccg
    15601 ccttcgcccg cgcggatgga tgctctgctg aaagctaaca cattgcgcct gtcgctgacc
    15661 tttaaccctg cacatgcgca gcaaaaaatc gccagcggcg atttgcctgc aagcagttac
    15721 agttttggtt ttcgcgaggg gatgattggc aacgtccatt tcatcaccat tcctgccaac
    15781 gcgaatgcca gtgctgcggc gaaggtagtt gccaatttcc tgctctcacc cgatgcgcaa
    15841 ctgcgtaaag cagatcccgc tgtctggggc gatccttctg ttctcgatcc gcaaaaactg
    15901 cctgacgggc agcgcgaaac attgcaatcg agaatgccgc aggatctgcc gccggtactg
    15961 gctgaaccgc acgcaggttg ggtaaatgcg ctggaacaag aatggctaca ccgttacggt
    16021 acgcattaat ctttttgctg tgggcgatga tggcggtgat ttatgcaccg ctgatcccgg
    16081 cagctctcac gctgatatcg cttgctttgt cgttgacgca ctggcaggcg ttatttgccg
    16141 atccacagtt accgcaggca ttactggcga cgctggtgtc gacaaccatt gcggcggtcg
    16201 gggcgttgtt gattgccctg ctggtgattg tggcgctgtg gccagggccg aaatggcagc
    16261 gtatgtgcgc ccggctgccg tggctgctcg ccattcccca tgtggctttt gccaccagcg
    16321 cccttctgct ctttgctgac ggagggctgc tttatgacta tttcccgtat ttcactccgc
    16381 caatggacaa attgggtatt gggctcggcc tcaccctggc ggtgaaagaa agcgcatttc
    16441 tgctgtggat cttagcggca gtattgagcg aaaaacggct gttgcagcaa ctcattgtgc
    16501 tggattcgct gggctacagc cgctggcaat gcctgaactg gctgctgttg ccctccgtcg
    16561 cgtctgcgct ggcaatggcg atgctggcga ttgtcgcctg gtcgctgtcg gtcgtggatg
    16621 tggcgattat tcttgggcca ggtaatcccc cgacgctggc ggtaattagc tggcagtggt
    16681 taactcaggg cgacgccgat caacaaacga aaggcgcact cgccagcctg ctgctgatac
    16741 tgttactcgc cgcctacgtt ttgctgggct atctgctatg gcgtggctgg cggcgtacta
    16801 ttccccgcgt agatggcgtt cgtaagcctg ccacgccttt attgccaggc aatacgctgg
    16861 cgagtttttt acccttaacc ggtgtgctgt gtgtggttct gctggcgatc ctcgcggatc
    16921 agtcgacgat caatagtgaa gcgctcatca acagcctgac aatggggctg gtggcggcat
    16981 tcatcgcttt gctcctgtta ctgctgtggc aggaatgggg gccacagcgt cgccagttgt
    17041 ggctatggtt gcccatttta ttacccgctc tgcctctggt ggcgggccag tacacgctgg
    17101 cgctatggct gaacctggat ggaagctgga cggcggtggt ctgggggcat ctgctgtggg
    17161 taatgccgtg gatgctgttt atcctgcaac ccgcctggca gcgcattgat tcacggttaa
    17221 ttttgattgc gcaaacactg ggctggtcgc gggccaaaat cttcttttac gtaaaatgcc
    17281 cactcatgtt gcgccctgcg ctgattgcct tcgcggtggg attttcagtc agtattgcgc
    17341 agtatatgcc gacgctgtgg ctgggcgcgg ggcgttttcc gacgctcacc actgaagcgg
    17401 tggcattaag cagcggcggc agcaacggta ttctcgccgc ccaggcttta tggcaactgc
    17461 tgttatcgct tattattttt gccctgaccg cgttagtcgc aaaatgggta ggttatgtca
    17521 gacaaggact ccgctaatgc tctgcgtgaa aaatgtttcg ctacgtttac cagaaagccg
    17581 cttgctgaca aacgttaact tttcagtaga taaaggtgac attgtcacgt taatgggacc
    17641 atccggctgc gggaaatcca ctctgttttc gtggatgatt ggtgcgctgg ccggacagtt
    17701 ttcttgtaca ggggagctat ggcttaatga gcaacggatt gacatgctac ccaccgcaca
    17761 gcgtcagatt ggcattcttt tccaggatgc actgttattt gaccagttca gcgtcgggca
    17821 aaatttactg ctggcgctac cggcgacact taaggggaat gcccgacgta atgccgtgaa
    17881 tgatgcactt gagcgttcag gccttgacgg agcgttccat caggatcctg ccactttgtc
    17941 tggcggtcag cgagcgcgcg ttgctctgct gcgcgccctt ctcgctcagc caaaagcgtt
    18001 gctactggat gagcccttca gccgtcttga tgtggccctg cgcgataatt ttcgccattg
    18061 ggtgttcagc gaaattcgcg ccatggcgat ccctgttgtg caggtgacgc acgatcttca
    18121 ggatgttcct cctgatagcc ccattctgga tatggcgcag tggtcagaaa attacaacaa
    18181 actgcgataa cgcaaagttt ttcccgatgc gtcagttcag aatggcgctc tcaaaactac
    18241 aatgtcggga ttttcgatga aacgtgtttc tcaaatgacc gcgctggcaa tggctttagg
    18301 gctggcttgc gcttcttcgt gggccgctga actggcgaag cctcttacac ttgaccagct
    18361 tcaacaacaa aatggcaaag cgattgatac tcgccccagc gcgttttata acggctggcc
    18421 acaaacctta aatggccctt ccggtcatga acctgccgcc ttaaacctct ctgctagctg
    18481 gctcgacaaa atgagcaccg aacagctcaa cgagtggatc aagcaacata acctgaaagc
    18541 cgatgccccg gtggcgctgt acggtaatga caaagatgtc gacgccgtca aaacgcgact
    18601 gcaaaaagca ggttttacgc atatctccat ccttagtgac gcgctaagcg aaccttcccg
    18661 tctgcaaaaa ctgccgcact ttgaacagct ggtatatccg cagtggctgc atgacctgca
    18721 acaaggtaaa gaggttacgg cgaaacctgc cggtgactgg aaagtcattg aagcggcctg
    18781 gggcgctcca aaattttacc ttatcagcca tattcccggc gctgactaca tcgacaccaa
    18841 cgaagtggaa agcgaaccgc tgtggaacaa agtttctgat gaacaactga aagcgatgct
    18901 ggcaaaacac ggcattcgcc atgacaccac ggtcattctg tacgggcgtg acgtatacgc
    18961 tgcagcgcgt gtggcgcaaa ttatgcttta tgccggcgtg aaagatgtgc gcctgctgga
    19021 tggaggctgg caaacctggt ccgacgcggg tctgcccgtt gagcgcggaa cgccaccgaa
    19081 agtgaaagcg gaaccggatt tcggcgtgaa gatcccggca caaccgcaac tgatgcttga
    19141 tatggaacaa gcgcgtggac tgctgcatcg ccaggatgca tcgctggtga gcattcgttc
    19201 gtggccagaa tttatcggta cgaccagcgg ttacagctat attaaaccaa aaggtgaaat
    19261 agccggagca cgttggggac acgcaggtag cgactcgacg catatggaag atttccataa
    19321 cccggatggc accatgcgca gcgccgatga tataaccgct atgtggaaag catggaatat
    19381 caaaccagat cagcaagttt cattctactg cggcactggc tggcgcgcgt cagaaacctt
    19441 tatgtacgca cgcgcaatgg gctggaataa cgtctccgtt tacgacggtg gctggtacga
    19501 atggagcagc gatccaaaaa atccgatagc aaccggtgaa cgcggcccgg acagcagtaa
    19561 ataacattgc aattactgac gctggagcga cttcagcgtc agatacccac tccacacccg
    19621 cgtaaaggtc gtcatccagc acaacgcacc aaatacccac gcaaaccacg gaaaacaggc
    19681 cggaaagagg caactcagaa caaacagcaa aatcgtctcg ctgccttcgg ttaacccgcc
    19741 cagataataa aacgatttat gcgcataacc aggattatca atctgatgtt tcgccgccag
    19801 cgcagcaaaa gcgagaaaac tgctgcccgt gccgataaac gcaaacagca gccagccgcc
    19861 taccagggcg ttttgctccg gtgcggcgag gataaaccca aaaggcacca gcgcgtaaaa
    19921 aagaaaatcg agagaaatat cgagaaagcc gcccgcgtcg gtaagccctc tgcgccgcgc
    19981 cagcgcaccg tccagaccat caagcagcct gttcaacaaa atggcgacta gtgccgccag
    20041 ataccagccc agcgccagaa aaggcagtgc cagcacgccg atggcaaatc ccactaatgt
    20101 taaaccatct ggcgtaatgc ccggtttatc aagaacccgc acgcactgat gcaacaacgg
    20161 tttaatccgg ggatgaagat ggcggtctag cacaggcact ccttaaatat aaagcctttc
    20221 tgattgagca acagtgcgga tattatggca tttttcgctt atctgcccgt gtgtaattta
    20281 tgaaaatgat tgaagttgtt gccgccatca tcgaacgtga tggcaaaatt ttactcgcgc
    20341 aacgccctgc ccatagcgat caggcgggat tatgggagtt tgccggtggt aaagtcgagc
    20401 cggatgaaag ccagcggcag gcgctggtgc gtgagttaaa cgaagaactg ggcatcgaag
    20461 caactgttgg tgactatgtt gccagccatc agcgagaagt ttcggggcgg attatccatc
    20521 ttcatgcctg gcacgtaccc gacttccacg ggacgttaca ggcacatgaa catcaggcgc
    20581 tggtctggtg ctcacctgaa gaggcgctgc gatatccgct ggcccctgct gacattccat
    20641 tattagaggc gtttatggct tcacgcgccg ccagaccagc ggattagtgc ttagggtttt
    20701 gtcatcccgt tggcattgca gcagtattcc ttcggcttta attactgccc cttcagaata
    20761 attttgatcc tgataaacgc agcactgagt acagggctgc gctgactgcc cgcctgaact
    20821 aaacacttct ggcggtacgt ttacctccac gtccggatga taatgcgggt tagccagtgt
    20881 aattaatgga aatgctaata ctacggcgat caatgctcga ctcacaggga actccttaac
    20941 gttattgtct cttctacaga taacggtagc cgggcggcaa aactttagcg tctgagttat
    21001 cgcatttggt tatgagatta ctctcgttat taatttgctt tcctgggtca tttttttctt
    21061 gcttaccgtc acattcttga tggtatagtc gaaaactgca aaagcacatg acataaacaa
    21121 cataagcata atcgtattaa tatataaggg ttttatatct atggatcaga catattctct
    21181 ggagtcattc ctcaaccatg tccaaaagcg cgacccgaat caaaccgagt tcgcgcaagc
    21241 cgttcgtgaa gtaatgacca cactctggcc ttttcttgaa caaaatccca aatatcgtca
    21301 gatgtcatta ctggagcgtc tggttgaacc ggagcgcgtg atccagtttc gcgtggtatg
    21361 ggttgacgat cgcaaccagg tacaggtcaa ccgtgcatgg cgtgtgcagt tcagctctgc
    21421 catcggcccg tacaaaggcg gtatgcgctt ccacccttca gtaaaccttt ccattctcaa
    21481 attcctcggc ttcgagcaaa cctttaaaaa tgccctgaca accctgccga tgggcggtgg
    21541 taaaggcggc agcgactttg atccgaaagg gaaaagcgaa ggcgaagtta tgcgtttctg
    21601 ccaggcgctg atgactgaac tgtatcgcca cctgggcgcg gatactgacg ttccggcagg
    21661 tgatatcggc gttggtggtc gtgaagtcgg ctttatggcg gggatgatga aaaagctctc
    21721 caacaatacc gcctgcgtct tcaccggtaa gggcctttca tttggcggca gtcttattcg
    21781 cccggaagct accggctacg gtctggttta tttcacagaa gcaatgctaa aacgccacgg
    21841 tatgggtttt gaaggaatgc gcgtttccgt ttctggctcc ggcaacgtcg cccagtacgc
    21901 tatcgaaaaa gcgatggaat ttggtgctcg agtgatcact gcgtcagact ccagcggcac
    21961 tgtagttgat gaaagcggat tcacgaaaga aaaactggcg cgtcttatcg aaatcaaagc
    22021 cagccgcgat ggtcgagtga cagattacgc caaagaattt ggtctggtct atctcgaagg
    22081 ccaacagccg tggtctgtac cggttgatat cgccctgcct tgcgccaccc agaacgaact
    22141 ggatgttgac gccgcgcatc agcttatcgc caatggcgtt aaagccgtcg ccgaaggggc
    22201 aaatatgccg accaccatcg aagcgactga actgttccag caggcaggcg tactgtttgc
    22261 accgggtaaa gcggctaatg ctggcggtgt agcaacatcg ggcctggaaa tggcgcaaaa
    22321 cgctgcgcgc ctgggctgga aagccgagaa agttgacgca cgtttgcatc acatcatgct
    22381 ggatatccac catgcctgtg ttgagcatgg tggtgaaggt gagcaaacca actacgtgca
    22441 gggcgcgaac attgccggtt ttgtgaaggt tgcggatgcg atgttaagcc agggcgtgat
    22501 ttaagacaat tgtgccggat acagcgcaaa cgccttatcc ggccataaaa atcttcagta
    22561 tcagatggtt gaaagaacct ttatgttatc actccattgc ccgcctcttc tgatggcggg
    22621 ctttttttcg gcgctctctt gcgtggcgaa tcctttttct tcccttctct accgccggga
    22681 gccattattc cccgaaactg ccttaccgac gtgcgctttg cctgatcaat caactggtac
    22741 aatgtcccca ccaacggctg cataaagtcc tgatagcgac actgcttttc gctgatttgc
    22801 gtcagcaccg attcccagtg cgcggtcatg tccggtcgcg tcgccatttc tggcagcgaa
    22861 tggaatagcg cttttccggc gtcggtggag tggatatagc gccctttttt ggtcaggaaa
    22921 ccccgcttga acaacagttc aataatcccg gcacgcgttg cttctgtccc cagaccatcg
    22981 gtcgcacgaa ggatcttttt cagatctttg tcctgcacaa agcgtgcgat cccggtcatc
    23041 gccgaaagca gtgttgcatc ggtaaaatgg cgtggcggct gggtttgccg ctctaccact
    23101 tcaccttttt cgcacaacaa ttcatcgcct ttcgccacca caggcagcgg cgtgccgtcg
    23161 ttttcttcat cgcgctcttt gctgcctaac agcgtgcgcc agcctgcttc agcaagaaaa
    23221 cgggctttag cgacaaattt gcctttggca atgtccagtt cgataacaca cttgcggaac
    23281 accgcatccg ggcagaactg catcaaatac tgacgggcaa tcaggttata gaccttcgct
    23341 tcgttctccg tcaggttgat cgcagaactc cgtgcggtcg gaatgatggc gtggtgcgca
    23401 tcgacctttt tgtcatccca acagcggttg cgtatatctg gatctaccac tggctgcggc
    23461 aacagatccg gtgcatgaac gctgatagca ttcatcaccg cgtggcgtcc ggcaaaatgt
    23521 tcttctggca aatagcggca atcggaacgc ggataagtga ttagcttgtg cgtttcgtac
    23581 agcttctggc agatatcaag cacgttctgc gcgctcagac caaaacgttt agccgcttca
    23641 atctgcaacg ccgaaagcga aaaaggcagc ggcgcggatt ctgattcccg tttatcgtta
    23701 tagctggtga caatagctgg ttgaccacta atgcggttaa ccacatgctc cgccagtgga
    23761 cgatgtaaca agcgcccttc ttcatcctgg tacggttcac acgcttcgct cggttgccag
    23821 atagcggtaa accgctcatc tgctggtgtc acgatatgtg ctttgacttc aaagaagtct
    23881 ttcgccacga agttttcaat ctcttcgtcg cggcgcacca ccagtccgag tacgggcgtc
    23941 tgcacgcgtc ccacggaaag cacgccctga taaccggcat tgcggccgag aatggtatac
    24001 gcacgggtca tattaatgcc gtacagccag tcagcccgcg cccgcgccag tgcagaaacg
    24061 cacagcggta caaactcgct attggagcga agacgatcga tcgcccgctc aaccgcctgc
    24121 gggttcaggt cgtttatcaa acaacgctgt acctgctggc gcttttccgg tgccagttgc
    24181 aggtagtcca gcacttcatc caccagcagt tgcccttcgc gatccgggtc tccggcgtga
    24241 acgatttcgc tggcttcatg caggaagcgt ttgatgacat taagttgttt agtcacggag
    24301 ggtcggggct gtaattgcca cttttccggg acaattggca aatccgcaag attccagcgc
    24361 gcatagcggc tgtcgtaggc gtctggctgc gcctgctcaa gcaggtgacc gatacaccag
    24421 gtcaccacct gaccattacc gcactcgata aagccatcgc ctttccggtg cggtttaggc
    24481 aggacatcag caatggcgcg cgccagactc ggtttttcgg caataaacaa ccgcattgaa
    24541 ttaacggatc tcaaccatgg cacgaccgcc gcgcgctggc accagttcgc caatcgccgt
    24601 cagttcaatg ccaaactcgg cggctgtagc tttgacctca ttttctgctt ccggcatgac
    24661 cgccagcagc aaaccgccag aagtttgcgg atcgcacagc agatcgcgca cttcacgcgg
    24721 catttcaccc atcagatgac cgtagctggc aaagttacgt tcggtgccgc caggcactgc
    24781 gcccaactta atgtactctt caacccccgg caatttcggg atcgcgtcat agtcgacgcg
    24841 cgcctgcaca cctgcgccct gacacatttc gcttaaatgc cccagtaaac caaaacccgt
    24901 aacgtcggtc atcgctttta cgccttcgat gttggcaaag gacgcgcctg cgatgttcat
    24961 ccggcacatc acttccgtcg ccagtccctg atgttctggt ttcagcagtg attttttctc
    25021 agccgtggta agaacgccaa tccccagcgg tttcgtcagg aacagtttgc atccggcttg
    25081 tgcggtactg tttttcttca cccgctcggt cggtacgatc cccgttaccg ccagaccaaa
    25141 aatcggctcc ggcgcatcga tggagtgacc gccagccagc gcaatacccg cctgacggca
    25201 tgcatagcgt ccaccttcgg tcacttcgcg ggcaatttcc ggggaaagtt tgttaatcgg
    25261 ccagccgagg atcgcaatcg ccataatcgg tttgccgccc atagcaaaga tatcgctgat
    25321 ggcgttagtc gccgcaatgc ggccaaaatc gaaaggatta tcaacgatcg gcataaagaa
    25381 gtcggtggta ctgataacgc tggtgccatt gcccagatcg tataccgccg catcgtcgcg
    25441 ggtttcatta cccacaagca aattcggatc aacaaacttc gcctgctcac tgtgcaggat
    25501 ggtttccaac acttttgggg aaattttaca gccgcaacca gcgccgtggc tgtattgggt
    25561 caaacgaatc gagttctcgc tcatggacat ctcctgtcaa tgcaatccag gtatggtaac
    25621 cctcattcca tgaagtgata agtgagagtg tctgaattcc tgcgcctttg ctcacaatcc
    25681 agacagtttc gcgacaatta tcagaaataa gtcacaaacg gcgtcgggtc cgggacgttt
    25741 atcgacgtag atgctttcaa ctgcggcgta ccgaggtaga gaaaaccgac aattttatcc
    25801 tgctcacggc aaccgaatgc ttcacgcact accggacttt cagttaatgc accactgcgc
    25861 cagatgccgc caaacccctg ggcaactgct gccatttgca tcgccatgac cgcgcatccc
    25921 gcagacattt cctgttccca gcgcgggact ttatgatttt cttcgcattt cgccaccacc
    25981 gttatgatga gcggtgcgcg gaacggcgca ttacgcgctt tgtcgattgc tttgttatca
    26041 ctaccggcag caatcgcccc ctgctccagt acagcgctga aacgctcgcg cccttcccct
    26101 tcaatcacaa agaaatgcca cggttgcatg gacttatggt ccggcgcacg catacccgca
    26161 cgcaggatgt tttgcagttg ttcacccgtt ggcgcgggtt cagccaggcg ggaggcgcta
    26221 cggcgattga tcaatagttc gagtgcatcc atttgattaa ctcctgtcat gatatttatt
    26281 cacaaaatta acacgagagt ggattttgtt acagcccagt ccgcaattcc tgctgacaag
    26341 tgccggttgg gtcattacga taaccacatc tattgcgcct gaaacaggtg tgaccttaag
    26401 ttgggagaat acatgcgaac cctttggcga tttattgccg gattttttaa atggacgtgg
    26461 cgtctgctga atttcgtccg tgaaatggta cttaacctgt tctttatttt tctcgtgctg
    26521 gttggtgtgg ggatctggat gcaggtcagt ggtggtgatt cgaaagaaac ggccagtcgt
    26581 ggcgcactgc tgctggacat ttctggtgtg atcgtcgata aacccgacag ttctcagcgg
    26641 tttagtaaat taagccgcca gctgcttggt gccagttccg atcgtctgca ggaaaactca
    26701 ctgtttgata tcgtcaacac cattcgccag gcgaaggacg accgcaatat caccggtatt
    26761 gtgatggatc tgaaaaactt cgcaggcggc gaccagccgt ctatgcagta catcggcaaa
    26821 gctctgaaag agtttcgtga cagcgggaaa ccggtttatg ccgttggcga gaactacagc
    26881 caggggcaat attatctcgc cagtttcgcc aataaaattt ggctgtctcc gcaaggcgtg
    26941 gttgatctgc acggctttgc caccaacggt ctgtactaca aatcgttact ggataagctg
    27001 aaagtttcca cccatgtgtt ccgcgtgggt acgtataaat ctgccgttga accgtttatt
    27061 cgtgatgata tgtcgtcggc agcccgcgaa gctgacagcc gctggattgg tgagctgtgg
    27121 caaaactatc tgaatactgt tgctgctaac cgccagatcc ctgctcagca ggtattccct
    27181 ggcgcgcaag ggttgcttga gggtttaacc aaaaccggtg gcgataccgc gaaatatgcg
    27241 ctggaaaaca agctggtcga tgcactggca tcgagtgccg aaatcgaaaa aactctgacc
    27301 aaagagttcg gctggagtaa aactgataaa aattatcgcg ccatcagtta ttacgattac
    27361 gcattgaaaa cgccggcaga taccggtgac agtatcggtg tcgtcttcgc taatggcgca
    27421 attatggatg gcgaggaaac tcaggggaat gttggcggtg ataccactgc ggcacaaatc
    27481 cgcgacgctc gccttgaccc gaaagtgaaa gcgattgttc tgcgtgttaa tagtcccggc
    27541 ggcagcgtta ccgcgtctga agtgattcgc gctgaactgg cagcagcccg ggcagcgggt
    27601 aagcctgtgg ttgtatcgat gggcggcatg gcggcatctg gtggttactg gatttccaca
    27661 ccagctaatt acattgtggc taaccccagc accctgaccg gttctatcgg tatcttcggc
    27721 gtgatcacca ccgtagaaaa tagtctggat tcgattggtg ttcatactga tggtgtctca
    27781 acttcaccgc tggcagatgt ttctatcacc aaggcactgc cgccggaagc gcagcagatg
    27841 atgcagttaa gcattgagaa tggctataaa cgctttatca cgctggttgc tgatgcgcgt
    27901 cattcaacgc cggagcagat tgataaaatc gcccagggtc acgtctggac cggtcaggat
    27961 gcaaaagcta acgggctggt cgatagtctc ggggatttcg atgatgcggt cgccaaagca
    28021 gcagagctgg caaaagtgaa acagtggcat ctggaatact acgttgatga accgaccttc
    28081 ttcgacaaag tgatggacaa catgtctggt tctgtccggg caatgttgcc agatgcgttc
    28141 caggccatgt tacctgcacc gctggcctcg gtagcctcta ccgttaaaag tgaaagcgac
    28201 aagctggccg cgtttaacga cccacaaaac cgttatgcgt tttgcctgac ctgcgccaac
    28261 gtgcgttaag tcttgtacta agtggccgac agatcgtcgg ccacattact ttttacgtcg
    28321 acgaatcctc ttcccgctgt ctcgccacat atttccttat cctcatagta tcaggtgcgc
    28381 tccccctgcc tcacgtatat acttttgctc tttcgatatc attcatatca atatcatgca
    28441 aaagaaatca atttacgtcg cctacacggg cgggaccatc gggatgcagc gttccgagca
    28501 gggttatatt ccggtgtcag gtcatctaca acgccaactg gcgctgatgc cggaattcca
    28561 tcgcccggag atgccagatt tcaccattca tgaatatacg ccattgatgg attcttctga
    28621 tatgacgcca gaagactggc agcatattgc tgaagatatt aaagcgcact atgacgacta
    28681 tgatggtttt gtcattctgc acggcacaga cacgatggcg tataccgcct ctgcactgtc
    28741 gttcatgctc gagaatctcg gtaaaccggt cattgtgaca gggtcacaaa tcccgctggc
    28801 tgagttacgc tctgacggac aaattaatct actgaatgcg ttgtacgttg cggcgaatta
    28861 tcctatcaac gaagtaactc tcttttttaa taatcgactg tatcgtggca accgaaccac
    28921 caaagcccat gccgatggtt ttgatgcgtt tgcctctcca aaccttcctc cgttactgga
    28981 agcaggtatc catattcgtc gtttgaatac gccacccgcc ccgcacggtg aaggggaatt
    29041 gatcgttcat ccaatcacgc cgcaaccaat tggcgtagtg acgatttatc cggggatttc
    29101 tgctgacgtc gtgcgcaatt ttctgcgcca acctgtgaaa gcattgattc tgcgctcata
    29161 tggcgtgggt aatgcgccac aaaacaaagc cttcctgcag gaattacaag aagccagcga
    29221 tcgcggtatt gtggtggtca acctgacaca atgtatgtcc ggtaaagtga acatgggtgg
    29281 ttatgccact ggtaacgccc tcgcccatgc cggcgttatt ggcggtgcag atatgactgt
    29341 agaagccacg ctaaccaaac tgcattacct gcttagccag gaacttgata ctgaaaccat
    29401 tcgcaaggcc atgagccaaa acctgcgtgg cgaactgacg ccggatgatt aaggagactg
    29461 caatgccccc tcgcgccctg ttactggtcg atttacaaaa tgatttctgt gctggtggcg
    29521 cgctcgccgt gccggaaggt gacagtacgg tggatgtcgc taaccgcctg attgactggt
    29581 gccagtcgcg cggtgaagcg gttatcgcca gtcaggactg gcatccggcg aatcacggca
    29641 gttttgccag tcagcatggt gtagagcctt atacgccggg ccaactcgac ggtttgccac
    29701 aaaccttctg gcccgatcac tgtgtgcaga acagtgaagg cgcacaatta catccgttac
    29761 tgaagcaaaa agcgatcgca gcggtgttcc ataaaggcga aaatccttta gttgacagtt
    29821 acagtgcctt ttttgataac ggccgtcggc agaaaactgc gctcgatgac tggttgcgcg
    29881 ctcatgtaat caatgaattg atcgttatgg gtctggctac tgactattgc gttaagttta
    29941 ccgtgctgga cgcgttacag ttaggctata aggtgaacgt gattaccgat ggttgtcgtg
    30001 gcgtaaatat ccagccacag gacagtgcgc acgcgtttat ggaaatgtca gcagctgggg
    30061 caacgctata tacgctggca gactgggaag aaacgcagga gtaattttac gctggcctac
    30121 aattctgtac tggcattgta ggccagataa aacacatcag tgatacatct ggtaattgac
    30181 gccatcaacg aaagatttaa ttcacctcag aaatctcttc cagcgacacc ttccgcgttt
    30241 cgatgccaaa aatggagaga accagcgcac agagcactaa catgacacca agcaccataa
    30301 acaccgtgat cgacccataa tgtgttaata atgccgcaac gccatagggc gtgaagactg
    30361 cgacgatccg tcctacggca ttaacgaaac ccgaaccacg caggcgtaaa tgcgttggcc
    30421 aaagttccgg gatataaacc gccgacgcga agcaaacata catgtataaa aagaagatca
    30481 tcaccaggcc atagattaaa atcgcccact ctgttgtctg aattgaatag atatagccta
    30541 acacggcaat aataataagt aaggcggaac caaataatcg acgagggaaa tgatcaataa
    30601 ttaatgccgc aataaatatc cctaccggag cgccaatcat aataacagcg gtcattaata
    30661 ttgatttatc gacatcaatg ccggagttaa caaatatggt cggtatccat acggtgatgg
    30721 tataaagcga aatgttcatt gcaattaaaa cagtaatcgc gactaatgta cgtcgtaaca
    30781 tttccccttt aaacaggagc cagaaggtac ccttgattac cttaaccttg ctgttgctct
    30841 ggttcaaagt aagcgggggt aaacgaatac tcttctctct ttcaatttgc tgctctactt
    30901 cacgaagttg gctttctgca cctgcgattc gccctttccc tgccaaccat cgtggcgact
    30961 caataaagta tttacctgag agaaaccagg ctaacagtat gccaataccg cccagcagaa
    31021 acattatccg ccaactaaaa aaagcgataa ccaccacgcc tatcgccgca gacagcatgg
    31081 gcgaccagtt tccaacaaat gagagccgcg cggaccattt tccacgcacc gtcgcgggga
    31141 taaactccgt aaatgaggca tagccaacca taatcagcgc ccccattcct gttcccatca
    31201 ggaagcgaaa gaagatgagc cagtacatat cagggacaaa agcggcccct gttgccgcaa
    31261 taccgacgat gagaagattt atgcgaaacg ccctgcgccg cccaaagtag tcaccaataa
    31321 acccaccggt aagagagccg atgaaataac caaacattaa tgccgaggta aaggcggcat
    31381 tgaggaaatt atttgaccat ccattgctta ccagctttgc taagacgaca ttaccggagt
    31441 aactcaaaaa ccccgttaat aacagactaa agcttattat accaaatata cgataatgaa
    31501 accgggccaa aggcaagcgg tccagtcttg cgccaatttg atcatattgt tccattgggt
    31561 gcctcggatt tgctaaaaaa ggtagcaatt gcggcaaatt cacttgccgc atctttatca
    31621 tcattttatt tccaggcgca ggaacaaata atattgacaa tgtgtgctta aattaccaca
    31681 ccagctggat attattgtct ttgcaaaatg ctatccactc tgcacccggt gatttatcag
    31741 ttattatgta attaatatga ctaaagtcag ctaactggac aaaagctttg cgatcaaact
    31801 tagagtgatc aaccaataac gcaacttctg tcgcctgacg gatcattgtc tttttgattt
    31861 cagcttccgc ttcattagag tccagcgcgc cgctgttaat atcaagacct ttacaactca
    31921 ttaccatgat atcgacatgg tagcggctga tgatctcttt agtaattctt ccttgcagtg
    31981 aaagtgtatt tttgtttagt tccccacccg ttgaaacgac tttaatttct gactgagcca
    32041 gaacatgaat cgcttccgcc gagttggtta gcaacgtcag gtcactacgg tcctgtaaca
    32101 atttgagcaa ttccattacg gtactactgg aatcggctgc catggtggtt ttattgtcga
    32161 taaagggtag tgccttgcgt gcaataagct gcttctcttc ataaaacgat gaagcgcgct
    32221 tataaaaatg gatattctcc gtcaacatcg ctgtatttaa aacagcacca ccataggttc
    32281 tggtcaaaaa gccttcatct tccaacttct caagatcgcg gcgaatggtt tcttcggtta
    32341 cctgaaaaat cccactcaaa tttgagactg tcaccttttt atcgttggca accatttgct
    32401 taattgcctg aatcctgtct tttgccgcca cgattacacc cctgtatctt tttacatcac
    32461 attagcgcaa ttatcgcata accgatgttt actttcaaaa taacctgttt gaatcacaga
    32521 ttttcatcac agttttcaca gaaacagagg tgaatcgtgt tgagtattta acgctccagg
    32581 gcctctgcca tttccctcat caatgttgcg tctgcatccg ataagttgat attcagtgcc
    32641 gcgacatttt cacgtacctg ttccggcgca gtagccccac taagaatgga gattaaatca
    32701 ctctgtttta atatccacgc cagtgccaga gtgggaattg tgcactgata acgagcacag
    32761 agtggctgcc actgttcaag catatcaatc actttcagca tgttttcacg ctggaaccag
    32821 actttatttg cccgagcgcc gcccggaacg taatcacgcg tgatggtccc ggtcaacaat
    32881 ccctgctcta gcggagagta gacctgtacc acaatgccat tatcacgaca tagcggcagc
    32941 agttcgtttt ccattgcccg gtcgaggata ctgtatttcg cctgaataat atccagttca
    33001 ccatgttgca gatactcgcg gatatggtca gcatcgacgt tagcagcgcc tatagcgcga
    33061 attttccctt cggcctttaa cgcattaagc actgcgacag tttcagctat cggcgtaaaa
    33121 aatggcggca ccgactgcca gtgcgtcatg tagatatcga tgtaatcaat acccagacgt
    33181 tgcaagctgg cttctacctc ttcgcggata gatttcggag aaaggttttt atacaactgc
    33241 cgatcgccaa ctttgttgaa taaactccct tttcgttccc agacaatgcc gcatttggtt
    33301 tctactacaa cctgttcacg gggcagtttt tttaacgcct gaccaacgat aacttcacta
    33361 ttgccaaagt tatatcctgg cgcagtatca atcaggttaa tgccgcaacg atgggcttca
    33421 agaatcgtat caatgcatat ttgtcgatcg agatcgccat tccatgcagg accgccgcca
    33481 atagcccatg tccccaaccc cattcgcgaa agcgttatat ccgctgtgcc taaaggtatc
    33541 tttttcattt gccttcatta tccttcgtat tcttccagca attgttcgac cagctttctg
    33601 ttttttacac cggtggtggc gccaatgctt agaaccgaga tggccgccgt tgcattggca
    33661 aagcgtgcgc attctcgcag atttttgcct tctaacagtg ctgcaataaa acctgaagca
    33721 aagttatcgc ccgcaccaat ggtgtcgata gcggttattc ctgcgaccgc cggcaccttc
    33781 atcgtcatgt caccacgctt gataaagcag ccgtctttac ccgttttaat caccaccgtt
    33841 tttacgccgc acgcaagaaa gcagtcagca atttcatcca gtgtctcttt cccggtgagt
    33901 aattttgcct cggcaaaatt aggaaacaga taatcgacat aactcaatgc ttcgcaaata
    33961 tcattcagcg tttcattcaa ccgcggtttg atcatatcgg cacagataat catctgccgg
    34021 gctttagctt gtgtaaaaat ttctgttagc gctctaccat ccaatagagg actgttgaaa
    34081 atactggcca gtgataataa tttcgcctga gaaaaccgcg caaaatcaac atcgtcaata
    34141 ttcaatttcc acagactgcc gttacggttt gtgacaaagg tccgctcgcc atcctccgtc
    34201 accagtccaa cgttaataga ggtatctatg ctgacgtcct gcttcaggct ttgaatatca
    34261 atattctctt tgcggcaatg gtcgagaata aattgccctg cggcatcttt accaatacga
    34321 ctcattaatg ctgtgcgatg gcccagacga gaaataattg ttgcttcatt tattgcatca
    34381 cccccggtgg tcattgcgat tctttcaaga ggataagaat ccacatcaaa gatattttta
    34441 ctgaccggtt gcaatggaat atcaacaata gcggcaccta tacaaataac gtcgagatta
    34501 tccatatcat tattccgctt tgccatcaga accgaacagt ttaattttat ccaatgcccg
    34561 ttcttttaca gctttacgca cttcacgttc taaatgcagg aaaggctgat cctggttttc
    34621 tttcactgct accattgcgg cctgacagag ctccgtatga at
//
LOCUS       DAZFFE010000038        34527 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFFE010000038 DAZFFE010000000
VERSION     DAZFFE010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.038"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(18..743)
                     /gene="phoU"
                     /locus_tag="ACHJ69_002073"
     CDS             complement(18..743)
                     /gene="phoU"
                     /locus_tag="ACHJ69_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO5015062.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDRDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAEKDSDK"
     gene            complement(758..1531)
                     /gene="pstB"
                     /locus_tag="ACHJ69_002074"
     CDS             complement(758..1531)
                     /gene="pstB"
                     /locus_tag="ACHJ69_002074"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO5015063.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(1622..2512)
                     /gene="pstA"
                     /locus_tag="ACHJ69_002075"
     CDS             complement(1622..2512)
                     /gene="pstA"
                     /locus_tag="ACHJ69_002075"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO5015064.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMIAAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2512..3471)
                     /gene="pstC"
                     /locus_tag="ACHJ69_002076"
     CDS             complement(2512..3471)
                     /gene="pstC"
                     /locus_tag="ACHJ69_002076"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO5015065.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(3558..4598)
                     /gene="pstS"
                     /locus_tag="ACHJ69_002077"
     CDS             complement(3558..4598)
                     /gene="pstS"
                     /locus_tag="ACHJ69_002077"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO5015066.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(4912..6741)
                     /gene="glmS"
                     /locus_tag="ACHJ69_002078"
     CDS             complement(4912..6741)
                     /gene="glmS"
                     /locus_tag="ACHJ69_002078"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO5015067.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGYMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(6902..8272)
                     /gene="glmU"
                     /locus_tag="ACHJ69_002079"
     CDS             complement(6902..8272)
                     /gene="glmU"
                     /locus_tag="ACHJ69_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO5015068.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKQQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGNRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(8623..9042)
                     /gene="atpC"
                     /locus_tag="ACHJ69_002080"
     CDS             complement(8623..9042)
                     /gene="atpC"
                     /locus_tag="ACHJ69_002080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO5015069.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(9063..10445)
                     /gene="atpD"
                     /locus_tag="ACHJ69_002081"
     CDS             complement(9063..10445)
                     /gene="atpD"
                     /locus_tag="ACHJ69_002081"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO5015070.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(10472..11335)
                     /gene="atpG"
                     /locus_tag="ACHJ69_002082"
     CDS             complement(10472..11335)
                     /gene="atpG"
                     /locus_tag="ACHJ69_002082"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO5015071.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(11386..12927)
                     /gene="atpA"
                     /locus_tag="ACHJ69_002083"
     CDS             complement(11386..12927)
                     /gene="atpA"
                     /locus_tag="ACHJ69_002083"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO5015072.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(12940..13473)
                     /gene="atpH"
                     /locus_tag="ACHJ69_002084"
     CDS             complement(12940..13473)
                     /gene="atpH"
                     /locus_tag="ACHJ69_002084"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO5015073.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(13488..13958)
                     /gene="atpF"
                     /locus_tag="ACHJ69_002085"
     CDS             complement(13488..13958)
                     /gene="atpF"
                     /locus_tag="ACHJ69_002085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO5015074.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(14020..14259)
                     /gene="atpE"
                     /locus_tag="ACHJ69_002086"
     CDS             complement(14020..14259)
                     /gene="atpE"
                     /locus_tag="ACHJ69_002086"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO5015075.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(14306..15121)
                     /gene="atpB"
                     /locus_tag="ACHJ69_002087"
     CDS             complement(14306..15121)
                     /gene="atpB"
                     /locus_tag="ACHJ69_002087"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO5015076.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(15130..15510)
                     /gene="atpI"
                     /locus_tag="ACHJ69_002088"
     CDS             complement(15130..15510)
                     /gene="atpI"
                     /locus_tag="ACHJ69_002088"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO5015077.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(16127..16750)
                     /gene="rsmG"
                     /locus_tag="ACHJ69_002089"
     CDS             complement(16127..16750)
                     /gene="rsmG"
                     /locus_tag="ACHJ69_002089"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO5015078.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(16814..18703)
                     /gene="mnmG"
                     /locus_tag="ACHJ69_002090"
     CDS             complement(16814..18703)
                     /gene="mnmG"
                     /locus_tag="ACHJ69_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO5015079.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGYNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(19082..19525)
                     /gene="mioC"
                     /locus_tag="ACHJ69_002091"
     CDS             complement(19082..19525)
                     /gene="mioC"
                     /locus_tag="ACHJ69_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO5015080.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEETGLTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKIEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(19615..20073)
                     /gene="asnC"
                     /locus_tag="ACHJ69_002092"
     CDS             complement(19615..20073)
                     /gene="asnC"
                     /locus_tag="ACHJ69_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO5015081.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            20225..21217
                     /gene="asnA"
                     /locus_tag="ACHJ69_002093"
     CDS             20225..21217
                     /gene="asnA"
                     /locus_tag="ACHJ69_002093"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO5015082.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(21222..22673)
                     /gene="viaA"
                     /locus_tag="ACHJ69_002094"
     CDS             complement(21222..22673)
                     /gene="viaA"
                     /locus_tag="ACHJ69_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO5015083.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKMIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(22667..24163)
                     /gene="ravA"
                     /locus_tag="ACHJ69_002095"
     CDS             complement(22667..24163)
                     /gene="ravA"
                     /locus_tag="ACHJ69_002095"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO5015084.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGALVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQITDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPSEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            24386..26254
                     /gene="kup"
                     /locus_tag="ACHJ69_002096"
     CDS             24386..26254
                     /gene="kup"
                     /locus_tag="ACHJ69_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO5015085.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            26421..26841
                     /gene="rbsD"
                     /locus_tag="ACHJ69_002097"
                     /pseudo
     CDS             26421..26841
                     /gene="rbsD"
                     /locus_tag="ACHJ69_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
     gene            26849..28354
                     /gene="rbsA"
                     /locus_tag="ACHJ69_002098"
     CDS             26849..28354
                     /gene="rbsA"
                     /locus_tag="ACHJ69_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO5015086.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            28359..29324
                     /gene="rbsC"
                     /locus_tag="ACHJ69_002099"
     CDS             28359..29324
                     /gene="rbsC"
                     /locus_tag="ACHJ69_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO5015087.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            29349..30239
                     /gene="rbsB"
                     /locus_tag="ACHJ69_002100"
     CDS             29349..30239
                     /gene="rbsB"
                     /locus_tag="ACHJ69_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO5015088.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            30365..31294
                     /gene="rbsK"
                     /locus_tag="ACHJ69_002101"
     CDS             30365..31294
                     /gene="rbsK"
                     /locus_tag="ACHJ69_002101"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO5015089.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKTLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            31298..32290
                     /gene="rbsR"
                     /locus_tag="ACHJ69_002102"
     CDS             31298..32290
                     /gene="rbsR"
                     /locus_tag="ACHJ69_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO5015090.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(32256..33683)
                     /gene="mdtD"
                     /locus_tag="ACHJ69_002103"
     CDS             complement(32256..33683)
                     /gene="mdtD"
                     /locus_tag="ACHJ69_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO5015091.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDTTGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIWLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(33706..34398)
                     /gene="yieP"
                     /locus_tag="ACHJ69_002104"
     CDS             complement(33706..34398)
                     /gene="yieP"
                     /locus_tag="ACHJ69_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO5015092.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAIKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVIRICLEPQACLLAAKVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         8319 a         8908 c         8812 g         8488 t
ORIGIN      
        1 gggatttact tggtgaatta tttatcgcta tctttttctg ccagcagttt atcgagctcg
       61 tcaccaccga cgtgacggaa atcctgcccc ttcacgtagt agaagataaa ctcacaaata
      121 ttctggcagc ggtcgccaat acgttcgata gaacgcgcac agaataacgc ggtaagtacg
      181 ctcggaatgg tacgcgaatc ttccatcatg taggtcatca gttgacgaac aataccttcg
      241 tactcctgat cgactttttt atcttcacga taaatacgta ccgcttcgtc aatgtccatc
      301 cgcgcgaacg cgtccagcac gtcgtgcagc atctggatgg tatgacggcc cagcgactcc
      361 agacttacca gcaacggctg atgctgctgg gagaatttct ccagcgcagt acggcagatt
      421 ttgtccgcca cgtcgccaat acgctccagc tcggcaatgg ttttactgat caccatcacc
      481 agtcgcaggt cgctcgccgt cggctggcgt ttggcgataa tgcgcacgca ggcttcatcg
      541 atcgccactt ccatcatgtt gacgtttttg tcgccttcga tgacgcgctt cgccagatct
      601 ctgtcctggt tatgcatcgc ggtgattgca tcagaaagct gctgctccac catgccgccc
      661 atggtcatca cctgcgtgcg gatgctttcc agttcggcgt tgaactggcc ggaaatatgt
      721 ttattaagat tgagactgtc cataacgcac tcctgaatca accgtaacga ccggtgatgt
      781 agtcttctgt ttgtttcttc gctggcttgg tgaacagatc gtccgtgttg ctgaactcaa
      841 tcaattcgcc caggtacata aacgccgtgt ggtcggaaca acgcgcagcc tgctgcatgt
      901 tgtgggtgac gatcaccacg gtgtaatcct gcttcagttc ggtgatcagc tcttcaatac
      961 gcccggtaga gatggggtcg agtgccgaac acggttcgtc gagcagcagc acttccgggc
     1021 gaatggcgat accacgcgca atacacaaac gctgttgctg accaccagag agagagtaac
     1081 cgctctggtg caatttatct ttggtttcgt tccacaatgc ggctttggtc aatgcccact
     1141 gcacgcgctc gtccatgtca gcacgggaga gcttctcaaa cagacgcacg ccaaaagcga
     1201 tgttgtcgta gatggacatc gggaacggcg tcggtttctg gaacaccatg cccactttcg
     1261 cacgcagcag ggcgatatcc tgagagttgg tcaggatgtt gtcgccatcg agcagaattt
     1321 caccttccgc acgctgttcc gggtacagtt caaacatttt gttgaaggta cgcagcagcg
     1381 ttgatttacc gcagccggac ggcccgataa acgccgtaac ctggtttttg gcgatatcca
     1441 ggttgatgtt tttcagggca tggaatttgc cgtagtagaa gttcaaatta cgaacctgaa
     1501 ttttactcgg ggcagtttca accatactca ttgcaatctt ttcctcatcg tgcctgatgc
     1561 acttcgcgaa tcaggcttac cgtaggccgg ataaggcgtt tacgccgcat ccagcaaagt
     1621 gttaaccgtg tttattcttc gcaaaaacaa cgcgcgccag aatgttcagc agcagtacgc
     1681 acagggtaat gatcaacacc ccggcccagg ccagttgctg ccattccgca aacgggctca
     1741 tcgcaaactt aaagatcgtc accggcaggt tggcgatcgg ctgcatcatg tccgtgctcc
     1801 agaactggtt ggagagcgca gtaaacagca gcggtgcggt ttcaccagca atacgggcaa
     1861 tcgccagcag gataccggtc atgatcccgg acaccgacgc tttcagcgtg atcgcggcga
     1921 tcatcttcca cttcggcgta cccagcgcat aagccgcttc acgcaggctg tccggcacca
     1981 gtttcagcat gttctcggtg gtgcggataa caatcggcac ctgcaacagc gccagcgcaa
     2041 tcacgcccgc ccagccggag aagtgctcca tctgcgccac cacgatggtg taaacaaaaa
     2101 gaccaaccac aatcgacggt gcggagagca aaatgtcgtt aatgaagcga atcacttctg
     2161 ccagccagga tttacgacca tattcagcca gataaatccc cgccataatg cccagcggcg
     2221 taccgaatac cgtggcccac aaaattaaca ggccgctacc cgccagggcg ttcgccagac
     2281 caccgccttc cgtatttggc ggcggcgtca tttcggtgaa cagcgccagc gacataccat
     2341 cgataccgcg agtgatggtg gacattaaaa tccagatcag ccagaacaga ccgaaggcca
     2401 tcgtcgccat cgagagcgtc agcgcaatac ggttcttaag gcggcgacgc gcctgcattt
     2461 tacggcggga ttcagccagc gccgcagtgg tttgcatttc aaccatagcc attagcgtgc
     2521 cccctcattc ttagccaggc gcataatcat aaacttcgat gcggcgagga cgatgaaggt
     2581 aatcacaaac aggatcaggc ccagttccat cagtgcagca acgtgcagac ccgactctgc
     2641 ttccgcaaat tcgttggcca gcgcagaggt gatactgttg cccggcatgt atagcgaggc
     2701 gctgtcgagt tggtaggtgt taccgatgat aaaggtcacc gccatggttt caccgagcgc
     2761 gcgccccagc cccaacataa tgccgccgat aacaccattt ttggtgaacg gaagaacgat
     2821 acgccagata acttcccagg tggtgcagcc aataccgtag gccgactctt tcatcatcac
     2881 cggggtttgt tcgaatacat cgcgcattac cgccgcaatg tacggaataa tcatgatggc
     2941 gaggatcacg cctgccgcga ggataccgat accaaaggcc gggccggaga acagcgcgcc
     3001 gacaatcggg atattcgaca tgatattgcc gaccggctcc tggaagtaaa cggcgaacag
     3061 cggcgcaaag ataaacaggc cccacatgcc gtaaacgata cttggaatgg cagccagcag
     3121 ctcaatggcg atacccagcg ggcgtttcag ccagccaggc gcaagctcgg tcaggaacag
     3181 ggcgatacca aaactcaccg ggacggcgat cagcagcgcg ataaacgagg tcaccagcgt
     3241 accgtagatc ggcaccagcg ccccgtagat atcgttcggc gcatcccact ctttggtcca
     3301 caggaaagcc agaccaaatt tctgaatgct cggccaggat gagatgatca gagagacaat
     3361 aatgccaccc aacatcaata gcacaatcag cgccgccagt tttaccagca cgctgaaaat
     3421 tatgtcgccc tttttacccg gtgggttaaa agcaggcttg gttgcagcca taagttactc
     3481 ttcagttaaa cgcgtttaca aagtcatttt gtaatgccgg atgcggcgta aaacaccgta
     3541 cccggcctgg agttttatta gtacagcggc ttgccgctac tgtctttaat attggtcttc
     3601 cacgcagcgc gaacctgttc aactacgcta tccggcaggc tggcataatc caggtcgttc
     3661 gcctgtttag ccccggtttt gtatgcccag tcgaagaact tcagcacttc ggtgccttgt
     3721 tctggtttct tctgatcttt atggatcaga atgaacgtgg tagaggtaat cggccatgca
     3781 tcttcacctt tctggttggt cagatcctga gcgaatgttt tgctccagtc tgcgcctttt
     3841 gctgcattag cgaagttttc ttcggtcgga ctaaccggtt tgccatctgc ggagatcagt
     3901 ttggtgtacg ccaggttgtt ctgcttcgcg taagcatatt caacgtaacc aattgcaccc
     3961 ggcagacgct gaacgaacgc ggcgataccg tcgttacctt taccgcccag accgatcggc
     4021 cattttacgg tagagccagt accaacgtta tttttccact cttcgttcac tttcgccagg
     4081 tagctggtga agacgaagga agtcccggag ccatctgcgc ggcgtactac agcaatgttc
     4141 tgtgaaggca gtttcagacc cggattcagt ttggcgatgg cttcatcatc ccacttcttg
     4201 attttgccca ggtagatgtc gccgagggtt ttaccatcca gcaccagttc gccagacttc
     4261 agacctggaa tattaaccgc cagcaccacg ccgccaatca cggtcgggaa ctggaacagg
     4321 ccttcctgag ccagtttttc gtcagacagc ggcgcgtcag aagcaccaaa atcaacggta
     4381 ttggcgatga tctgttttac accaccagaa gaaccgatac cctggtagtt aactttatta
     4441 ccggtttctt tctggtaagt gtcagcccat ttggcataca ccggcgcagg gaaggttgca
     4501 cctgcacctg tcaggcttgc ttctgcaaac acagagaaag cgctcatcga taaggtcgcg
     4561 gcgacaacag ttgcgacggt ggtacgcata actttcataa tgtctcctgg gaggattcat
     4621 aaagcattgt ttgttggcta cgagaagcaa aataggacaa acaggtgaca gttatatgta
     4681 aggtttatga cagttttatg acagagagag aaagtcttta gtctgattta aataagcgtt
     4741 gatattcagt taattacaaa tattaataac gaagagatga caaaaaaatt ttcattctgt
     4801 gacagagaaa aagtagccga agatgacggt ttgtcacatg gagttggcag gatgtttgat
     4861 taaaaacata acaggaagaa aaatgccccg cttacgcagg gcatccattt attactcaac
     4921 cgttaccgat tttgccaggt tacgcggctg gtcaacgtcg gtgcctttga tcagcgcaac
     4981 gtggtaagcc agtagttgca gcggaacggt gtagaagatc ggtgcaatca cctcttccac
     5041 atgcggcatc tcaatgatgt gcatgttgtc actgctcaca aaacccgcat cctgatcggc
     5101 aaagacatac aactgaccgc cacgcgcgcg aacttcttca atgttggatt taagtttttc
     5161 cagcaattcg ttgttcggtg caacgacgat aaccggcata tcggcatcaa tcaacgccag
     5221 cggaccgtgt ttcagttcgc cagcagcgta ggcttcagcg tgaatgtaag agatctcttt
     5281 cagcttcaat gcaccttcca gcgcgattgg gtactgatcg ccacggccca ggaacagcgc
     5341 gtgatgtttg tcagagaaat cttctgccag cgcttcaatg cgtttgtcct gagacagcat
     5401 ctgctcaata cggctcggca gcgcctgcag accatgcacg atgtcatgtt caatggaggc
     5461 atccagacct ttcaggcgag acagtttcgc caccagcatc aacagcacag ttaactgggt
     5521 ggtgaatgct ttggtggagg ccacgccaat ttccgtaccg gcgttggtca tcagcgccag
     5581 atcggattcg cgcaccagag aagaacccgg aacgttacag attgccagcg aaccaaggta
     5641 acccagctct ttcgacagac gcaggcccgc cagggtatcc gcggtttcgc cagactgtga
     5701 caaggtgatc atcaggctgt tacgacgcac ggcagatttg cgatagcgga attcagaggc
     5761 gatttcgacg tcgcacggaa tacctgccag cgattcaaac cagtagcggg aaaccatacc
     5821 ggagttataa gaagtaccac aggcgaggat ctgaatatgc tcaaccttcg acagcagttc
     5881 atcggcgtta ggtcccagct cgcttaaatc aacctgaccg tggctgatgc gcccggtaag
     5941 ggtgtttttg atcgcgttcg gctgttcgta gatctctttc tgcatgtagt ggcggtaaat
     6001 acctttatcg cccgcgtcat attgcagatt ggattcgata tcctgacgtt ttacttccgc
     6061 gccagtttta tcgaagatgt ttaccgaacg gcgagtgatt tccgcaatat cgccctcttc
     6121 aaggaagata aaacgacgag tcactggcaa cagcgccagc tggtcagaag cgataaagtt
     6181 ttcgcccata cccaggccaa tcaccagcgg actaccagaa cgtgccgcca gcagggtatc
     6241 cggatgacgg gagtccatga tcactgtacc gtacgcacca cgcagttgcg ggatagcacg
     6301 cagaactgcc tcacgcagag taccgccttg tttcagctcc cagttcacca aatgtgcaat
     6361 cacttcggtg tcggtttcag aaacgaaggt atagccacgc gcttttaact cttcacgcag
     6421 cggttcatgg ttttcgatga tgccgttatg caccaccaca atgtgttcag aaacatgcgg
     6481 atgcgcgtta gcttctgaag gttcaccgtg ggtcgcccag cgagtatgag caataccggt
     6541 gccgccatgc agaggatgtt cttccgctgc ctgagccagc atctggactt taccgaggcg
     6601 acgcaggcgg gtcatataac cttctgcatc aacaacggcc agaccggcag agtcatatcc
     6661 gcggtattcc agacgacgta aaccttcaag aaggatttct gctacatcac gttgcgcgat
     6721 cgcgccaaca attccacaca tagtttttga ttccgattta tatcgttgtc ggtcaacctg
     6781 tatgcccgtt tttcgggcgc cccgagcctt gtagagagtg ggggtatttt tatagttact
     6841 gcttgtgggc gggagattat gttatctcct catcccatat gaccggatta cccggccaga
     6901 attacttttt ctttaccgga cgacgccagc cttctttttg agtctgcggc acacggctga
     6961 tagccagggc attttcgccg acattacgcg tcacagttgt accagcagca atggtcgcgc
     7021 ctttgcctac tgttaccggg gccaccagct gagtatcgga tccaacaaac acatcgtcgc
     7081 cgataatagt cttaaattta ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga
     7141 tgttaacgtt atcgccaatt tccgcatcgc ccaggtaagt cagatgacca gctttcgagc
     7201 ctttacccag acgcgctttt ttcatctcaa cgaagttgcc gacgtgtgcg ccttccagca
     7261 actcagcacc aggacgcaga cgggcaaacg ggccaatggt acaggccgct gccagattcg
     7321 catcttccac aacggtatac gggctaattt cgcaatcatc gccaatcacg ctgtttttaa
     7381 tcacacaacc ggtgccgatt ttcacacgat taccgagagt cacgttgccc tcgatgataa
     7441 cgttagtatc aatttcaaca tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg
     7501 ccggatcgcg cagcataacg cctgctaaca gtagtttttc agcctgttcg gactggtaaa
     7561 cgcgctccag tcgggagagt tgcaggcggt tattcacgcc ttctacttcg cttaaacgtt
     7621 gcggatgaac ggcgacgatt tcacgccctt cctgatacgc cagcgcaata atgtcggtga
     7681 tgtagtattc accctgagcg ttattgttgg tcagcttcgc cagccagcgt ttcatatctg
     7741 cgccgttggc aatcagaatg ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg
     7801 cgtctttgtg ctcaacaatg ccggtaactt tgccgttttc acgggtgata cgtccataac
     7861 cggtcggatc atccagtttt accgtcagca gaccaatgcc accctgctgt ttagcatcac
     7921 gcaggcgctg gagtgtttcg acagagatca gcggcacgtc gccgtagagc attaaaatgt
     7981 cttcatcatc ggcaaagaaa ggtgcggcct gctgcatcgc atgacctgta cccagctgtt
     8041 ctgcctgaag cacccagttc aggttgtcat ctttcagcgc ctgtttaagc agatcgccgc
     8101 cgtgaccgta aaccaggtga acgtgcgctg cgcctaattc attcgcagca tcaatgacat
     8161 gctgaaccat cgctttcccg gcaagggtat gcagcacttt cggaagatcg gaatacatgc
     8221 gcgtgccttt gcctgcggca aggatcacta cgctcatagc attattcaac atacgcgtcc
     8281 tgactgtaat ttgagaacga atttaaaccg cttcaccttg aaaaaactac atttttttca
     8341 tcgtgaaatg gacagaggat aaattgttca ataacggatt atccccgctg acgaaagcgc
     8401 cattttcgac catcgtcacg ccttttgtct gcagaaaatt aaggcagtac cttcatttaa
     8461 gcgtcaggtg gatgtttttg ctcttatttc gatcaatgaa taaacgggaa aacagggtgt
     8521 tttatatcag caggatctat gtgaacgctc ttcaggacgg gtcaacgcgt aaaaaaaagc
     8581 cagcctgtta ccagactggc ttttgtgctt ttcaagccgg tgttacatcg cttttttggt
     8641 caactcgata acgcgcagct gcgcgatcgc tttggccagt tccgcagacg cctgagcgta
     8701 atctacgtcg ccgtgagagc tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc
     8761 tcgcgcttcg tcgagatcct ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc
     8821 aggctgcact tcaagaatgc cgccagacag atagataaac tcttcgtgac cgtgctgttt
     8881 cacgatgcga atcataccag gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat
     8941 ccccagttca ccttcgctac ccgttacctg gattttctcg accagaccag agaacatttg
     9001 ttgctctgcg ctgacgacgt ccaggtggta agtcattgcc atatcaccct ccgattaagg
     9061 cgttaaagtt ttttggcttt ttccacagct tcttcgatgg aaccgaccat gtagaacgcc
     9121 tgctccggca ggtgatcgta ttcgccttcc atgatgcctt taaagccacg gatggtgtct
     9181 ttcagggaga cgtatttacc cggagaaccg gtgaatactt ctgccacgaa gaacggctgg
     9241 gacaggaagc gctggatctt acgagcacgc gctaccacca gtttgtcttc ttcagacagt
     9301 tcatccatac ccaggatggc gataatgtct ttcagttcct gataacgttg caggatggac
     9361 tgaacgccac gcgcggtgtc gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg
     9421 ctggtggagt ccagcgggtc aacggccggg tagataccca gagacgcgat ctgacggctc
     9481 agtaccacgg ttgcgtcaag gtgcgcaaag gtggttgccg gagacgggtc agtcaagtca
     9541 tccgcaggta cgtatactgc ctgtacggag gtgatagaac cggttttggt ggaggtgata
     9601 cgttcctgca gaacgcccat ctcttccgcc agggtcggct gataacctac cgctgaaggc
     9661 atacggccca gcagtgcgga tacttccgta ccggccaggg tgtaacgata gatgttgtca
     9721 acgaacagca gaacgtcacg accttcgtca cggaatttct cagccatggt cagaccggtc
     9781 agagcaacgc gcagacggtt tcccggcggc tcgttcatct ggccatacac cagggatact
     9841 ttgtcgataa cgttggagtc ggtcatttcg tggtagaagt cgttaccctc acgagtacgt
     9901 tcacctacgc ccgcaaacac agagtaaccg gagtgctcga tcgcgatgtt acgaataagc
     9961 tccatcatgt ttacggtttt acctacaccc gcaccaccga acagaccaac tttaccgccc
    10021 ttagcgaacg gacacatcag gtcgataact ttgataccgg tttccagcag ttcctgagag
    10081 tttgacagct cttcgtagga aggtgctgcg cggtgaatcg cccaacgctc ttcttcaccg
    10141 atctcgcctt tcatgtcgac cggttcaccc agtacgttca tgatacggcc cagagtcgct
    10201 ttacctaccg gaacttcgat cgggtgttcg aggtctttta catccagacc gcgacgcaga
    10261 ccgtcggagg aacccattgc gatggtacgc acgataccgc cgccgagctg ctgctgaact
    10321 tccagcacca gacgctcatt accattttgc acctcaagag catcgtacac gcgcggtacg
    10381 gcatcctgag ggaattcgac gtcaactacg gcgccgatta cctggacaat ctttccagta
    10441 gccatcttaa atcctctacg aaataacctg tttaaaccgc ggcggccccc gagacgatct
    10501 cggtgagttc ctgagtaatg ctggcctgac gagctttgtt gtataccaac tgcagctctt
    10561 taatcaggct gccgccattg tcggtcgcgg ctttcatcgc caccatacgg gcggcctgct
    10621 cgctggccag gttttcaacc acgccctgat aaacctgaga ttcgacataa cgacgcagca
    10681 gggtatccag caacgccttc ggatcgggtt cgtacaggta atcccaggat ttatgtttca
    10741 gatcatcatc atctgatgcc ggtaacggca gcagctggct gatggtcgga acctgagaca
    10801 tggtgttaat aaatttgttg ctgacaatgt aaagcttgtc cagacggcct tcgtcgtagg
    10861 cctgcaacat cacttttacc ggaccgatca gttcggacag ggaagggtta tcccccatgc
    10921 cggtgacctg ggcaacaaca ttgccgccca cggagttgaa gaacgacacg cctttcgagc
    10981 cgatcattgc gaggtcgcat tgaacgcctt tgtcggtcca ggtcttcatt tccgccagca
    11041 gttttttgaa caggttaatg ttcaaaccac cgcacaaacc acggtcggtc gacaccacca
    11101 ggtagcccac gcgtttaacg tcgcggtctt ccaggtaagg gtgcttatat tccagattac
    11161 cgtgtgcaag gtgaccaatc actttgcgca tggtttctgc ataaggacgg ctggccgcca
    11221 tgcgatcctg cgatttacgc attttggaag cggcgaccat ctccatcgct ttagtgatct
    11281 tttgcgtgtt ctggacgctt gcgatcttac tacgtatctc ttttgcgccg gccatgagct
    11341 tctcctcaat gccttgcggc ctgccctaag gcaagccgcc agacgttacc aggattgggt
    11401 tgctttgaag gaatcgagga tgcctttcag cttgccttcg atttcgtcgt tgtagccacc
    11461 ggtctggttg atctcttgca tcaacggagc gtgatcacgg tcgacgtaag ccagcagagc
    11521 ggcttcgaag ctgccgattt tcgacagttc aacatccgcc aggtaaccac gttctgctgc
    11581 gaacagaacc agagactgtt gcgctacaga catcggcgca tactgtttct gtttcagcag
    11641 ttcggtcact ttctgaccgt ggtcaagctg cttacgtgtt gcatcgtcaa ggtcggatgc
    11701 aaactgagag aacgctgcca gttcacgata ctgtgccaga gcggtacgga taccaccgga
    11761 cagttttttc atgatcttgg tctgtgctgc accaccaaca cgggatacgg aaatacccgg
    11821 gttaaccgca ggacgaatac cggcgttgaa caggttggtt tccaggaaga tctgaccatc
    11881 ggtaatggag attacgttgg tcggaacgaa cgcagaaacg tcacccgcct gagtttcgat
    11941 aatcggcagc gcagtcagag aaccggtttt ccctttcact tcacctttgg tgaaagcttc
    12001 aacgtattcg gcgttaacac gcgcagcacg ctccagcaga cgagagtgga ggtagaatac
    12061 gtcgcccggg aatgcttcac gtcctggcgg acgacggagc agcagggaga tctgacggta
    12121 agcaacagcc tgtttagaca ggtcatcgta aatgatcagc gcatcttcac cgcggtcacg
    12181 gaagtattca cccattgcgc aaccagcgta cggtgccagg tattgcagtg ctgcggattc
    12241 agacgcggtt gctaccacaa cgatggtgtt agccagtgcg ccgtgctctt ccagtttacg
    12301 taccacgtta gaaatggtgg acgctttctg gccgatagcg acatagatac atttgatacc
    12361 ggaatcgcgc tggttgatga tggcatcgat agccagtgcg gttttaccgg tctgacggtc
    12421 accgatgatc aattcacgct gaccacgacc gattgggatc atggagtcaa cggctttata
    12481 accggtctgt accggctgat ctacggactg acgttcgata acgcccggag cgattgcttc
    12541 tacagcagag aagccgtcgt gatccagcgg acctttaccg tcgattggtg cacccagagt
    12601 gttaaccaca cggcccagca ggccacggcc aaccggaact tccaggatac ggccagtaca
    12661 cttaactttc atgccttcgg caaggtcagc gtacggaccc ataacaaccg cacctacaga
    12721 gtcgcgctcg aggttcagtg cgatagcgta acggttaccc ggcagggaga tcatttcacc
    12781 ctgcatacaa tcggccaggc cgtgaatgcg gataacaccg tcacttacag aaacaatagt
    12841 accttcgttg tgagcttcac tcacaacatt gaactgagca atgcgctgct tgatcagttc
    12901 gctgatttcg gtggaattca gttgcatgct ccagtcccct taagactgca agacgtctgc
    12961 aaggcgctca agacgaccgc gtacgctgcc atcaatgacc atatcacccg ctcggatgat
    13021 aacgcctgcc attacagact tatcgatttt gcaattcagc ttaactttgc gtgacagacg
    13081 tttttccatc gcagcagaaa ttttcgcgag ctgttgttca ctcaacgcgg cagcggaaat
    13141 gacgtctacc tcagcggtag cctcactcac agcacgcagg tgaataaact gctccagaac
    13201 atccgggagc gcgttaagac gaccattttc ggccattacc cgaatcaggt tctgaccgtt
    13261 ttcgtccagt tgctcaccac aaactgcgat aaacgactcg gcgagcgttt ctggcgcaag
    13321 cgcgccagag agaagctctg ccatttgttc gtttttggtt acctcggcgg caaacgccag
    13381 catgtcctgc cagcgttcta cactttggtg ttcgacggca aagtcaaaag ctgctttggc
    13441 gtaggggcga gctaccgtaa taaattcaga catcagcccc tccctcctta cagttcagcg
    13501 acaagtttat ccacgatgtc gctgttagca gcttcatcca cggaacgttc gatgatcttc
    13561 tcggcgccag caacagccag gatagcaact tgcttacgca gctcttcacg ggcacgttta
    13621 cgctcggctt caatttccgc ctgcgcctgg gccacgattt tagtacgttc ctgttctgcc
    13681 tcagctttcg cttcgtccag aatctgcgag cggcgtttgt tagcctgctc gatgattacc
    13741 tgggcttccg ctttcgcttt tttcagctgg tcggtcgcgc tggcctttgc aaggtcaagg
    13801 tccttatgtg ctcgttctgc ggaagcaagg ccgtcagcaa tttctttttg acgtttttcg
    13861 atggctgcca ttaatggcgg ccatacgtac ttcatgcaga acagaacgaa caggacaaac
    13921 gcgatggcct ggccgaggat tgttgcgtta agattcacag cacaatgcct ctatttagtt
    13981 aacgttctga tattgctctt taaataaaag caacgcttac tacgcgacag cgaacatcac
    14041 gtacagaccc agacctacag cgatcatcgg gatagcatcc accagaccca taacgataaa
    14101 gaactgagta cgcagcagag gaatcagatc aggttgacgc gctgcgcctt ccaggaattt
    14161 acccccgagg atgccgatac cgatcgcagc accgattgcc gccagaccca tcatcacagc
    14221 ggcagccatg tacagcagat ccatattcag gttttccatg acagtctcca gtttgtttca
    14281 gttaaaacgt agtagtgttg gtaaattaat gttcttcaga cgccatcgac agatagacga
    14341 tcgtcagaac catgaagatg aaggcttgca gcgtaatgat caggatgtgg aaaatggccc
    14401 acggcacatt caggatccac tgtgaccacc acggcaacag accagcaatc agaatgaaaa
    14461 tcagctcacc ggcatacatg ttaccgaaca gtcgcaaacc gagtgaaact ggtttggaca
    14521 gcaggcttac cccttcaagg attaagttga caggaatgaa cgcccagtga ttgaacggct
    14581 gcagcgtcaa ctctttcgtg aagccgccga tgcctttcat tttgatgctg tagaacagaa
    14641 tcaggataaa tacgcccagt gccatagaca gcgttacgtt cacgtcagca gacggaacca
    14701 cacgcagtgc aggcagaccc agtacatgtt cagcaatgta cggcagcagg tcgataggca
    14761 gtaaatccat caggttcatc aggaataccc agacgaagat cgtcagggcc agcggagcaa
    14821 tcagcttgct tttgccatgg tacatgtctt tcacgctacc attaacaaag ccgatcacca
    14881 gctcaatcgc ggtctgaaac ttacctggca cgccgctggt tgcctttttg gctacactac
    14941 ggaataaaac caggaacaac agacccagca ccaccgagaa gaacatggag tcaatattga
    15001 ttgtccagaa ggtggctggg gggttatgtg gatccaccag cgagaatgta cgcaggtcca
    15061 gctgaaggtt attcaggtgg tgtcctatgt aatcctgcgg cgtcatattt tctgaagcca
    15121 tgatgccttt taccctttgt tgttaattac agccggtgcc agtatctgaa ccaccagcac
    15181 caaaacccac gtaacgatca gcggcaagaa taccgccttt aaaaccgcca acgccaccac
    15241 cagtaacacc aacatcgcca gaactttgaa agcttcgcca aatgcgaatg tccaggccac
    15301 ccggcctttc gctggtgtat gcgcctggtg acgccaggca aatatcataa acaaaacgtt
    15361 aggcagaaag actgccaggc ccccgcttat tgcagagacg ccccagaagg ggtctttgag
    15421 gctgaacagc aatccacttg ctatcaccac cagtaactga acgagcagaa gcttccgagc
    15481 aacgtttcga ctcacgagcg acacagacat cacgtttttc actcctgctc ccttcgaggt
    15541 atgccgcgtg tcgtataaaa ctttctttaa ggcttagagt caagcatcaa aaagcggtca
    15601 aattatacgg tgcgcccccg tgatttcaaa caataagtag ccaaaaggtg aataaatgtt
    15661 taaatatttt tccagtgcat acaattgcga cttttctgca aaccctgttc gatcatgaaa
    15721 aactgtaaat aacgcgtaaa cactggtgat aaagcgtgct tcagatcaca tattgcgcat
    15781 gttcgcgcac agcatattta tttacttggc aaatgatgcc tttgcaagtt tatgatattt
    15841 cagtctaaaa acagatactg ttttaataaa tgacatttac ataacaaaaa ccacccattg
    15901 acatttttaa taatgtttta acagtcaatg atgattctta gcgccgattt ttagcagact
    15961 gatattttca cttatgactt attttctgct taccaaaaaa agccacgtta tcttgttgat
    16021 acaaaagagt gaacgtggcg ttaaatgtaa ccagttatat cagtagaaaa cctggttgtt
    16081 gttaacagtc taaccggtca attttttatg atttttttga taaaaattaa attttatttg
    16141 ctttaatcac caccagatga cgttcgccat ccagggctgg aacctgaagt ttaaccactg
    16201 attcgacctg atattcttcg ggcaacaaag cgatttcatc ttccggcatt tgccctttca
    16261 gcgcgtagaa acggccttgc tcaccaggaa gatggtggca ccagctcacc atatcgttca
    16321 gagaggcaaa agcgcggcta attacgccat caaatggcgg ctctgacgga aactcttcta
    16381 ccctgctttg tactggctca atattctcca gtttaagctc atgttgcacc tgacgaagga
    16441 aacgcacgcg tttaccaagg ctatccaaca acgtgaaatg agcttcagga cgcacgatag
    16501 agagtggaat gccaggcagt cccggtccgg tgccgacatc gataaaccgt tcaccttgca
    16561 gatacggtgc caccacaatg ctatcgagaa tatggcgtac cagcatctca ttaggatcgc
    16621 ggaccgaagt caggttgtac gctttattcc atttatgcag catattcacg taggcaataa
    16681 gctggttttt ctggtgatcg gtaagcgaaa tacctgcgtc tttcagcagt aaggagagtt
    16741 tgttgagcac ggtgattacc tgttcttgat gcgttgcctg gtaagcgggt gcttaccagg
    16801 catttttaat gcgttatgcg ctacgacgca gcataccctg ttttttcagc cacaccagca
    16861 gaatggagat agccgcaggc gtgacgccag aaatacgcga agcctggcca atagaggccg
    16921 gtttgtgatc gttaagtttg gcgatcactt cgttagaaag accggatacc tggcggtaat
    16981 ccagcgtcgc tggtagcagg gtattctcgt tacgcagctg cttttcgatc tcatcttgct
    17041 ggcgcgcgat ataaccttcg tatttaacct gaatctcaac ctgttccgca gcctgttcat
    17101 ctgtcaacgc aggggcaaac ggtgtcagcg tggttaattt ttcataagtc atttcaggac
    17161 gacgtagcag atcttcacca ctggcttcac gggaaagcgg cgcagtcagg tgagcattca
    17221 cttcggctgc agcttccgcc gacggggtta cccaggtcga tttcagacgc tgacgctcac
    17281 gctcgatatt ctcaagtttc tcgttaaagc gcgcccaacg ttcgtcatcc accaggccca
    17341 gttcgcgacc gatttcagtc aaacgcagat cagcattatc ttcgcgcagc atcagacgat
    17401 attctgcgcg cgaggtaaac atacgatacg gttctttggt tcctaaagtg cacaggtcat
    17461 caaccagtac gccgagatat gcctgagaac gcgccggagc ccaaccttct ttgtcagcag
    17521 acagacgggc agcgttaaga ccagccagca aaccttgcgc agcggcttct tcgtaaccgg
    17581 tagtgccgtt aatctgacca gcaaagaaca gcccctggat aaacttgctc tccagcgtcg
    17641 gtttcaggtc gcgtggatcg aagaagtcat actcaatggc ataacccgga cgcacgatct
    17701 ttgcgttttc catcccctgc atggagcgga cgatttgcat ctgcacatcg aacggcaggc
    17761 tggtggagat accgttcgga taaatttcgt tagaggtcag cccttccggt tcaaggaaga
    17821 tctgatgctg atttctgtcg gcgaagcgca tgactttgtc ttcgatcgac gggcagtagc
    17881 gtgggccgac accttcgatc acccctgcgt acattgggct acgatcgagg ttactgcgga
    17941 tcacatcatg ggttttctcg ttggtatgag tgatataaca cggcacctgc tggggatgct
    18001 gggacgcatt gcccataaac gagaatactg gcattgggtt atagccatgc tgttgcgcca
    18061 gtacgctaaa gtcgatggtt cgagcatcaa tacgcggtgg tgtcccggtt ttcagacgac
    18121 caacgcgcag cggcagttca cgcaaacggc gagaaagcgg aatggacggc ggatcaccag
    18181 cacggccacc gctgtaatta tccagaccga tatgaatttt accgtcgagg aacgtcccaa
    18241 cggtgagcac gacagctttg gcacggaact tcagtcccat ttgggtaacg gcaccgacca
    18301 cgcgatcgtt ttcgacaata agatcttcaa ctgcctgctg gaagatcatc aggttcggtt
    18361 ggttctccag cgccgtacgt accgcctgac ggtagagcac acgatctgcc tgagctcggg
    18421 tagcgcgaac cgccggtccc ttgcttgcgt ttagtatcct aaactggata cccgcctgat
    18481 cgatcgcttt cgccatcaga ccgccgagtg catccacttc ttttaccaga tgtcccttcc
    18541 caataccgcc gatcgccggg ttgcagctca tctgccccag agtgtcgata ttgtgtgtca
    18601 aaagcagagt ctgttggccc atacgcgccg cggccatcgc ggcctcggtg cctgcatgac
    18661 ccccgccaat gatgatgacg tcaaaaggat ccggataaaa catggtgatt gcctcgcata
    18721 aagcggtatg aaaatggatt gaagcccggg ccgtggattc tactcaactt tgtcggcttg
    18781 agaaagacct gggatcctgg gtattaaaaa gaagatctat ttatttagag atctgttcta
    18841 ttgtgatctc ttattaggat cgcactgccc tgtggataac aagtatccgg cttttaagat
    18901 caacaacctg gaaaggatca ttaactgtga atgatcggtg atcctggacc gtataagctg
    18961 ggatcagaat gaggggttat acacaactca aaaactgaac aaccgttgtt ctttggataa
    19021 ctaccggttg atccaagctt cctgacagag ttatccacag tagatcgcac gatctgtata
    19081 cttatttgag taaattaatc cacgatccca gccattcttc tgccggatcc tccggaatgt
    19141 cgtgatcaag aatgttgatc ttcagtgttt cgcctgtctg ttttgctccg gaatttttga
    19201 gttctgcctc gattttatcg atagccccac aaaaggtgtc atattcacga ctaccaatac
    19261 cgattgcgcc aaagcggacc gcagaaagat cgggcttctg ttcctgcaat gcttcataga
    19321 aaggagaaag gttgtccgga atatctccgg caccgtgggt tgagctgata accagccaga
    19381 tccccgaggc agataaatct tccaacagcg gaccgtgcag cgtttcggtg gttaaacccg
    19441 tctcttccag cttttcagcc aggtgttctg ctacatattc ggcaccgccg agggtgctgc
    19501 cgctgataag agtgatatct gccataaacc gccaccttta ttaagagtgg cgtattgtac
    19561 gctgtgaacg cgttgggatc tacctgtgga aaagtatggg attaaaaaag ccgatcaggg
    19621 cttaatggta cgcatgatcg ggttctgcag gacgatcagt gtctcggtgg actgaatttc
    19681 atcaattgtt tggatcttgt tgataagtac atgctggaga gcatcgatcg aacggcacat
    19741 cacttttata aagatgctgt agtggccggt tgtgtaatag gcttcagtga cttcatcgag
    19801 gctttccagc tttgccagcg cggaaggata gtctttggcg ctctttaata taatgccgat
    19861 aaagcagcct acgtcataac caagctgctt cgggctgaca tcaatacgcg ccccggtaat
    19921 gatccccgcc tgcttcattt tctctactcg aacgtgaatc gtccccggac tgacgccaaa
    19981 ttgtttcgcc agttcggcgt aagcggtgcg cgcattgccc attaatgctt ccaggatgcc
    20041 acggtccaga ttgtcgatca gataattttc cataggattt tcttatgcgg attgatgatt
    20101 cattctattt tagacttctt ttttaatgaa tcaaaagtac gtgaggcttt ttattgaatg
    20161 attattgcat gtgtgttggt ttttgttgct taatcataag caacaggacg caggagtata
    20221 aaaaatgaaa accgcttaca ttgccaaaca acgtcaaatt agcttcgtga aatctcactt
    20281 ttctcgtcaa ctggaagaac gtctggggct gatcgaagtc caggcgccga ttcttagccg
    20341 tgtgggggat ggcacgcagg ataacttgtc gggctgtgaa aaagcggtgc aggtaaaagt
    20401 gaaagctctg cctgatgccc agttcgaagt ggttcattca ctggcgaagt ggaaacgtca
    20461 gaccttaggg caacacgact tcagcgcggg cgaagggctg tacacgcaca tgaaagccct
    20521 tcgccccgat gaagaccgtc tttccccgtt gcactcggtc tatgtagacc agtgggactg
    20581 ggagcgcgta atgggcgacg gcgagcgtca attctcgact ctgaaaagca cggtagaggc
    20641 gatctgggcg ggaattaaag caaccgaagc tgcggttagc gaagagtttg gcctggcacc
    20701 gttcctgccg gatcagatcc atttcgtaca cagccaggag ttactgtctc gttatccgga
    20761 tctcgatgcc aaagggcgtg agcgggcgat tgcgaaagat cttggcgcgg tattcctcgt
    20821 cgggattggc ggcaagttga gtgatggtca tcgccacgac gtgcgcgcac cggattatga
    20881 tgactggagc accccgtcag agctgggcca tgcgggtctg aacggcgata ttctggtgtg
    20941 gaacccggta ctggaagatg cgtttgagct ttcttccatg gggatccgcg tggatgccga
    21001 cacgctgaag catcagctgg cgctgaccgg tgacgaagat cgcctggagc tggagtggca
    21061 tcaggcgctg ctgcgcggtg aaatgccgca gaccatcggc ggcggtatcg ggcagtctcg
    21121 tttgaccatg ctgctgctgc aactgccaca tatcggccag gttcagtgtg gtgtatggcc
    21181 ggcagctgtt cgcgagagcg tcccttctct gctgtaataa tttatcgccg ccagcgtctg
    21241 agcaggcggc ttcgcatccc ggtatcaaag cgccagatat gatcgaaaat gcgcatgatg
    21301 ccgggtttgc cgtgtgccga catcgccacg gcatgaaagc gatgctgatg tacccgctgc
    21361 aactctttca ctttactcgt cacgtcgtca ggcaaccgct gggcgataaa atcagaaatc
    21421 accaccgcat cggcatcaaa ccattccctg ctttgcaagc gttccataat ggcgcgaaaa
    21481 caactggcaa gatcggtgcc gccacgaaac tgctgactta aaaagcggat cgcttgctca
    21541 ataccttgtg gccctgaaag ctcataacgg acgatctcgg tggaaaatag cataatatag
    21601 cagcgccggt tttcagcgag ggctatgcgc atcaaggcca ggcagaacgc tttcgcacac
    21661 tgttcattaa agccgcccat tgagccggaa gtatccacgc agacaataaa cggtccgcgc
    21721 ggctgttcgt cgtaatcttt atgcaccacc gggcgttcga tcattttttc acgccacgac
    21781 tcaccgtgca ggcgataggt aagcaactgt ttttccacca gccgacggta aaactcatac
    21841 tccagttccg ttatccctag tgtcgccagt tctggcggca agagacgtaa aatatcatcg
    21901 ctttgctgca gaccatctac ctgctcagga actgtcgccg gttcgcgcac catggtgcgg
    21961 aaggtttcca tctgcgcatc gttgcgcggt attgatttgg cttcccggga acgccccagc
    22021 tgttctgcca gacgtttcag ttccggctgt tcgttaagaa attcaccgta tttcacaatc
    22081 aactgatagt cgccacgttt aagctgaccg gcactcatat cccacagacg accagctgcg
    22141 gtattgttat ctgcgagaat cggttcaagt tgcccgctca gcgtcatgcg ttcctgaact
    22201 tcactcaaca gttgttcgcg ttcttcttct aatagctgct gatttaacgt cgttgcttgc
    22261 acgatcaggc ttaaacgcca gcgctggaga aaaagcgtgt gtaacgctga agtgatcgta
    22321 ctgttagcat caaccaactg tcgggcttgt tctgcccatg gggaattcag acgatgcagt
    22381 aagtccagga tttgtggtag ctgcacaata aactgcggcg tggagaggag ctggctttgc
    22441 tgatagcaca tcacctcttc ggtgagttcc ggcgggactc gggcatcttt cagccgactg
    22501 cgcagcgcct cacgccagcg gggaacatca tcagtgattg ccgccttcag tcgtgggaat
    22561 ttttcaaaga aaacagccag ctgcggtgag gccagcagcg cgatgatcat ctcttcgatc
    22621 aatccctctt cgctgacggc cagcatcaca ttaagcgtat ccagcgttag cattgttgtg
    22681 cctggcgaat ctgtgcgccg acatcctgta ggctggcttc aatgcgacct aaccagtcgc
    22741 cagggataaa cagacatttt tgctgttcgc tgaataaagc gtgttgcttg cgccagtcgc
    22801 tttcaagctc ttccagttgt tgttttattt cgctgggcag accttcagcc gatgaaccgg
    22861 gaagtgctag cgtactgcct tgtaaactca catcgcgtac aaccagatgt tgggcgctat
    22921 cgacttccag attcagtttc tgggcaaagc cgataccgtt cagtttgccg cgaatctcac
    22981 cacctttact cagccactgt tccagcgcgc tacgctcaaa ggagatatga accacttcca
    23041 tatcatgcag ttttaacggt ttttgcagca gcagagtcag agtggaagca gtaacgttaa
    23101 cagggagttg atactgctga cgacggctaa aaatgccgcc cagacgaatt accgttaagg
    23161 ctgttttatc gctttgttgc tgctgaagtt gcaggtgacg ttgcacaatc gcgcccaggc
    23221 gggtcaacat cccttgctgt tgccaggcgt gaccggtcat caatacatca atttgttgtt
    23281 gtatcaaatt caggctttgc gcgtcatacc acaggcaatc tttcagcaaa atgagatcaa
    23341 ccggggcgac agcactgcga ccgctaaaaa aggcgctggc ctgcaataag cggatcgctt
    23401 ttttccagcg acgatccgag acataaggcg catccggtaa tttatccagt tgctggcgca
    23461 gcataaaaat cagctcaaat acatgatcgg gcagcgtaat ttcaccaatc tctttctgcc
    23521 agcgttcata ttcttcatct gtgatctgca aggaggcagg aaccggattg tcattttcat
    23581 cctgttgact ggtcagcatg gagcggaaat tcgctttatc ctgcacttta tctaaccaca
    23641 gacgaatcag catgcggtca tataacgctt ccagactgct gtctgcttcc ggcagctcgt
    23701 tggaggccgc caccagcaga cgcatcggga ttttttctac gagtgcgccg ttgcggaact
    23761 ggcgctcgtt aatggcggtg agcagggtat taagaattgc cggacccgct ttccagatct
    23821 catccagaaa gacgatttca gcttccggca ggtaaccgct ggttaaacgt tcatagcgcc
    23881 cttcatcttt tagcgcctga atagaaaggg gaccaaaaac ttcttccggc gtggagaagc
    23941 gggtcatcag atattcaaac gcgcgggcat tctgaaaggc gaattttaag cgccgggcga
    24001 tcaaactttt ggcaatacct ggtgggccaa ggaggaacac actttcacca cttaatgccg
    24061 ctaataaaca caagcggatg gcgtggctac gttcataaag ccccttttcc aacgaactgc
    24121 tcaggcggga aattctttcc gctaataaat gagggtgagc cataatgaag tggcgtcctt
    24181 tcgtcaaaag ttctgcgtaa attgcgagta tagacgtttc ttgctggtgg ctaaaatagt
    24241 ctcaaaggag gggtattttt ctttgagcca ggttaatgtg gccgcattta ggagtacgat
    24301 tttgccgtta atcgtgcata ctgtgcgctt ttttgtgggc caagggacta agcacacatt
    24361 tcatatttca acgaaagact agtctatgag cactgataat aagcaatcat tgcccgcgat
    24421 taccctcgcg gcgattggag ttgtctacgg cgatattggt accagcccgt tatatacact
    24481 tcgtgaatgt ttgtccggcc agtttggttt tggcgttgaa cgcgatgccg tgtttggctt
    24541 tttatcgctg atcttctggc tgctaatctt tgtggtttcc attaaatatc tcaccttcgt
    24601 gatgcgggca gataacgccg gtgaaggggg gatcctgacg ttgatgtcgc ttgccgggcg
    24661 taatacgtcg gcgcgaacca catcaatgct ggtgattatg gggctaatcg gcggcagctt
    24721 tttctatggt gaagtcgtca ttactcccgc tatttcggtg atgtccgcca ttgaaggtct
    24781 ggaaatcgtc gccccgcagc tggatacctg gatagttccc ctctcaatta tcgttctcac
    24841 attattattc atgattcaaa aacatggcac ggctatggtc ggtaagctgt ttgcgccgat
    24901 catgctgacc tggtttttga ttctggcagg gctggggtta cgtagcatta ttgctaaccc
    24961 ggaagtgctg catgcactga atccgatgtg ggcgatgcat ttcttccttg aatacaaaac
    25021 ggtttctttt attgcattag gggcagtggt gctgtcgatt acgggggtcg aggcgctgta
    25081 tgctgatatg gggcactttg gtaagttccc tattcgtctg gcgtggttca ccgtcgtatt
    25141 gccttcctta acccttaatt acttcggcca gggagcgctg ttgttaaaga atccggaagc
    25201 gattaagaac ccgttcttcc tgttggcacc ggactgggcg ctgatcccgc tgctgatcat
    25261 cgccgcactg gcgacggtaa ttgcctcgca ggcggttatc tctggcgtct tctcattgac
    25321 gcgtcaggcg gtacgtctgg gatatttgtc gccgatgcgc attattcaca cttccgaaat
    25381 ggagtcaggg caaatctata ttccctttgt gaactggatg ctctatgtcg cggtcgtgat
    25441 tgtgattgtc agctttgagc actccagcaa cctggcggcg gcgtacggga ttgcggtgac
    25501 cggaaccatg gtgctgacgt ctattctctc gactaccgtg gcacgtcaga actggcactg
    25561 gaataagtat tttgttgcgc tgatcctgat tgctttcctt tgtgtcgata ttcccttgtt
    25621 caccgctaac ctcgataaac tgctctccgg cggctggttg ccattgagcc tcggtacggt
    25681 gatgtttatc gtgatgacca cctggaagag cgagcgtttc cgcttgctgc ggcggatgca
    25741 tgaacatggt aactctctgg aagcgatgat tgcttcgctg gagaaatcac cgcccgttcg
    25801 cgtgcctggg accgcggtgt atatgtcgcg tgcaatcaac gtcattccct ttgcgctgat
    25861 gcataacctg aaacataaca aggtattgca tgagcgggtg attctgttaa ctttgcgcac
    25921 cgaagacgcg ccatatgtcc ataacgtccg tcgggtacag attgaacaac tgtcgcccac
    25981 tttctggcgc gtggtggcaa gttatggttg gcgagaaacg ccaaacgtgg aagaagtttt
    26041 ccaccgctgc ggtctggaag gattaagttg ccggatgatg gaaacctcct tctttatgtc
    26101 gcatgagtcg ttgatcctcg gcaaacgacc gtggtatttg cgtctgcgcg gcaagctgta
    26161 cttgctgctg caacgtaatg cgctgcgggc accggatcaa tttgaaatcc cgccaaacag
    26221 agttatcgaa ctgggtactc aggtcgaaat ctaacgccag acgcctcctt tctccataag
    26281 ggggcgtttt tgttttcatg gttaatcacc atgtaaaacg tttcgaggtt gatcacattt
    26341 ccgtaacgtc acgatggttt tcccaactca gtcaggatta aactgcgggt cagcgaaacg
    26401 tttcgctgat ggagaaaaaa atgaaaaaag gcaccgttct taattctgat atttcatcgg
    26461 tgatctccct actgggacat accgatacgc tggtggtgtg tgatgctggt ttacccatcc
    26521 ccaaaagtac aacgcgtatc gatatggcat taacccaggg tgtaccttct tttatgcagg
    26581 tgctgggtgt agtcacaaat gaaatgcagg tcgaggctgt cattatcgcg gaagagatca
    26641 aacaacataa tccgcaactc cacgaaacgt tgctcactca ccttgagcag ctgcaaaaac
    26701 accagggaaa caccattgaa attcgttaca ccacgcatga acaattcaaa caacaaaccg
    26761 cagaaagtca ggcggtaatt cgcagcggag aatgttctcc gtatgcgaat aatcattctc
    26821 tgtgctggcg tgacgttctg aggccgtcat ggaagcatta cttcagctta aaggcatcga
    26881 taaagccttc ccgggcgtaa aagccctctc gggcgcagcg ttaaatgtct atccgggccg
    26941 cgtgatggcg ctggtgggcg aaaacggcgc gggtaaatcc accatgatga aagtgcttac
    27001 tggtatctat actcgcgatg ccggtacgct tttatggttg gggaaagaaa cgacatttac
    27061 cgggccgaaa tcttcccagg aagccgggat tgggattatc caccaggaac tgaacctgat
    27121 cccgcagtta accattgccg aaaacatttt cctcggtcgt gagtttgtta atcgctttgg
    27181 caaaattgac tggaaaacca tgtatgccga agcggataaa ttgctggcta aacttaacct
    27241 gcgctttaaa agcgacaagc tggtgggcga tctttccatc ggtgaccagc aaatggttga
    27301 aatcgccaaa gtgctgagtt ttgagtcgaa agtcatcatt atggatgaac cgaccgatgc
    27361 gctgaccgat accgaaaccg aatccttgtt ccgcgtcatc cgcgagctga aatcgcaagg
    27421 ccgtggtatt gtctatatct cccaccgcat gaaagaaatc ttcgagattt gcgatgacgt
    27481 taccgttttt cgtgacggac aatttattgc tgagcgcgaa gtggcatcac tgaccgaaga
    27541 ttctctgatt gagatgatgg tgggccgcaa gctggaagat caatatccgc acctggacaa
    27601 agcgccggga gatatccgcc tgaaagtcga taatctctgc ggacctggcg ttaacgatgt
    27661 ctcttttact ttacgcaaag gcgaaattct tggcgtctct ggtttgatgg gcgctgggcg
    27721 taccgaactg atgaaagtgc tctacggtgc actgccgcgc acaagcggtt acgtcaccct
    27781 ggatgggcat gaagtcgtta cccgttcgcc gcaggatggc ctggcaaacg gcattgttta
    27841 tatctccgaa gaccgtaaac gtgacggttt agtgctgggc atgtcagtaa aagagaacat
    27901 gtcgctgacc gcgctgcgct acttcagccg tgctggcggc agtttgaagc atgccgatga
    27961 acagcaggct gtgagtgatt tcattcgtct gtttaatgtg aaaacgccat cgatggaaca
    28021 ggcaattggt ctgctttccg gtggcaatca gcaaaaagta gcgattgccc gtggtctgat
    28081 gacacgccct aaagtgttga tcctcgatga gcctacccgt ggcgtagatg tcggcgcgaa
    28141 aaaagagatc tatcaactga ttaaccagtt caaagccgat ggcttgagca tcattctggt
    28201 gtcatcggag atgccagaag tattaggcat gagcgatcgc atcatcgtca tgcatgaagg
    28261 gcatctcagc ggggaattta ctcgtgagca ggccacccag gaagtgttaa tggctgccgc
    28321 tgtgggcaag cttaatcgcg tgaatcagga gtaaaaaaat gacaacccag actgtctctg
    28381 gtcgccgtta tttcacgaaa gcgtggctga tggagcagaa atcgcttatc gctctgctgg
    28441 tgctgatcgc gattgtctcg acgttaagcc cgaacttttt caccatcaat aacttattca
    28501 atattcttca gcaaacctcg gtgaacgcca ttatggcggt cgggatgacg ctggtgatcc
    28561 tgacgtcggg tatcgacttg tcggtaggtt ctctgttggc gctgaccggc gcagttgctg
    28621 catctatcgt cggcattgaa gtcaatgcgc tggtggctgt cgctgccgct ctcgcgttag
    28681 gtgccgcaat tggtgctgta actggggtga ttgtagcgaa aggtcgcgtc caggcgttta
    28741 tcgctacgct ggttatgatg cttttactgc gcggcgtgac catggtttat accaatggta
    28801 gcccagtgaa taccggcttt actgagaacg ccgatctgtt tggctggttt ggtattggtc
    28861 gtccgctggg cgtaccgacg ccagtctgga tcatggggat tgtcttcctc gcggcctggt
    28921 acatgctgca tcacacgcgt ctggggcgtt acatctacgc gctgggcggc aatgaagcgg
    28981 caacgcgtct ttctggtatc aacgtcaata aaatcaaaat catcgtctat tctctttgtg
    29041 gtctgctggc atcgctggcc gggatcattg aagtggcgcg tctctcctcc gcacaaccca
    29101 cggcggggac tggctatgag ctggatgcta ttgctgcggt ggttctgggc ggtacgagtc
    29161 tggcgggcgg aaaaggtcgc attgttggga cgttgatcgg cgcattaatt cttggcttcc
    29221 ttaataatgg attgaatttg ttaggtgttt cctcctatta ccagatgatc gtcaaagcgg
    29281 tggtgatttt gctggcggtg ctggtagaca acaaaaagca gtaataacga ctacaggaca
    29341 tcttgaatat gaacatgaaa aaactggcta ccctggtttc cgctgttgcg ctaagcgcca
    29401 ccgtcagtgc gaatgcgatg gcaaaagaca ccatcgcgct ggtggtctcc acgcttaaca
    29461 acccgttctt tgtatcgctg aaagatggcg cgcagaaaga ggcggataaa cttggctata
    29521 acctggtggt gctggactcc cagaacaacc cggcgaaaga gctggcgaac gtgcaggact
    29581 taaccgtccg cggcaccaaa attctgctga ttaacccgac cgattccgac gcagtaggta
    29641 atgctgtgaa gatggctaac caggcgaaca tcccggttat cactcttgac cgccaggcaa
    29701 cgaaaggtga agtggtgagc cacattgcat ctgataacgt actgggcggc aaaatcgccg
    29761 gtgattacat cgcgaagaaa gcgggtgaag gtgccaaagt tatcgagctg caaggcattg
    29821 ccggtacatc cgcagcccgt gaacgtggcg aaggtttcca gcaggccgtt gctgctcaca
    29881 agtttaatgt tcttgccagc cagcctgcag attttgatcg caccaaaggt ttgaacgtaa
    29941 tgcagaacct gttgaccgct catccggatg ttcaggctgt attcgcgcag aatgatgaaa
    30001 tggcgcttgg cgcgctgcgc gcactgcaaa ctgccggtaa atcggatgtg atggtcgtcg
    30061 gatttgacgg tacaccggat ggcgaaaaag cggtgaatga tggcaaacta gcagcgacta
    30121 tcgctcagct acccgatcag attggcgcga aaggcgtcga aaccgcagat aaagtgctga
    30181 aaggcgagaa agttcaggct aagtatccgg ttgatctgaa actggttgtt aagcagtagt
    30241 tttaatcagg ttgtatgacc tgatggtgac ataaatacgt catcgacaga tgaacgtgta
    30301 atataaagaa aagcagggca cgcgccaccc taacacggtg gcgcatttta tggacatccc
    30361 gaatatgcaa aacgcaggca gcctcgttgt tcttggcagc attaatgctg accacattct
    30421 taatcttcaa tcttttccta ctccaggcga aaccgtaacc ggtaaccact atcaggttgc
    30481 atttggcggc aaaggcgcga atcaggcggt ggctgctggg cgtagcggtg cgaatatcgc
    30541 gtttattgcc tgcactggcg atgacagcat tggtgagagc gttcgccagc agctcgccac
    30601 tgataacatc gatatttctc cggtcagcgt gatcaaaggc gaatcaacag gtgtggcgct
    30661 gatttttgtt aatggcgaag gtgagaatgt catcggtatt catgccggcg ctaatgctgc
    30721 cctttccccg gcgctggtgg aagcgcaacg tgagcgtatt gccaacgcgt cggcattatt
    30781 aatgcagctg gaatcaccac tcgaaagtgt gatggcagcg gcgaaaatcg cccatcaaaa
    30841 taagactatc gttgcgctta acccggctcc ggctcgcgaa cttcctgacg aacttctggc
    30901 gctggtggac attattacgc caaacgaaac ggaagcagaa aagctcaccg gtattcgtgt
    30961 tgaaaatgat gaagatgcag cgaaggcggc gcaggtactg catgaaaaag gtatccgtac
    31021 tgtactgatt actttaggaa gtcgtggtgt atgggctagc gtgaatggtg aaggtcagcg
    31081 cgttcctgga ttccgggtgc aggctgtcga taccattgct gccggagata cctttaacgg
    31141 cgcgttaatc acggcgttgc tggaagaaaa aacattgcca gaggcgattc gttttgccca
    31201 tgcagccgct gcgattgccg taacacgtaa aggcgcacaa ccttccgtac cgtggcgtga
    31261 agagatcgac gcatttttag acaggcagag gtgacgcttg gctacaatga aagatgttgc
    31321 ccgcctggcg ggcgtttcta cctcaacagt ttctcacgtt atcaataaag atcgcttcgt
    31381 cagtgaagcg attacagcca aagttgaagc ggcgattaaa gaactcaatt acgcgccatc
    31441 agctctggcg cgtagcctca aactcaatca aacacatacc attggcatgt tgatcactgc
    31501 cagtaccaat cctttctatt cagaactggt gcgtggcgtt gaacgcagct gcttcgaacg
    31561 cggttatagt cttgtccttt gcaataccga aggcgatgaa cagcggatga atcgtaatct
    31621 ggaaacgctg atgcaaaaac gcgttgatgg cttgctgtta ctgtgcaccg aaacgcatca
    31681 gccttcgcgt gaaatcatgc aacgttatcc gacagtgcct accgtgatga tggactgggc
    31741 tccgttcgat ggcgacagcg atcttattca ggataactcg ttgctgggcg gagacttagc
    31801 aacgcaatat ctgatcgata aaggtcatac ccgtattgcc tgtattaccg gtccgctgga
    31861 taaaactccg gcgcgcctgc ggttggaagg ttatcgtgct gcgatgaaac gtgcgggtct
    31921 caacattcct gatggctatg aagtcactgg tgattttgaa tttaacggcg ggtttgacgc
    31981 tatgcgccaa ctgttatcac atccgctgcg tcctcaggcc gtctttaccg gaaatgacgc
    32041 tatggctgtt ggcgtttacc aggcgttata tcaggcagag ttacaggttc cgcaggatat
    32101 cgcggtgatt ggatatgacg atatcgaact ggcaagcttt atgacgccac cattaacaac
    32161 tatacaccaa ccgaaagatg aactggggga gctggcgatt gatgtactca tccatcggat
    32221 aacccagccg accctacagc aacaacgatt acaacttact ccgattctga tggaacgcgg
    32281 ttcggcttag atttacgctg tcttttgatc aaattattac catcggttgt tttcagtagc
    32341 atgaacattg ctgctgaagc aacagtaata atgcccatcg tgataaacgt atagtggaat
    32401 tgttcgactg tcgttgtgcc ttccatccct tcgtaaacgc gaaggacggc cgcacttaca
    32461 gcaacgccta aactgataga cagttgctgc gtgaccgcca gaacactgtt accgctgctg
    32521 gcgttgtcat cggtcagatc ggcaagtgtg atggtattca tcgcggtaaa ttgcgtcgac
    32581 atagccatcc ctaatataaa caacggcaag atcagcatcc atatcgccat tgctggtgat
    32641 tgcaaagaga actgagcgat cattagccca ataatcaccg tgatccccac taacgtatgg
    32701 cgatagccca gacgacgtaa gacttgggta accatcgatt ttgcaataat ggaacctaac
    32761 gctgtcggtg ccatcataca gccagcaata aacgcctgat acccaaatcc tacctgtaac
    32821 atcaatggca taaggaacgg tacacagccg gtccccagac gggttgcaat attgcctacg
    32881 ataccgatcg agaaagtgcg ggttttaaat aaatctaatg aaattaatgg gtttggcgtg
    32941 cgtcgcgcat ggagaatata gagaagcagt aaccagatgc tggtgacaat taccgtcaag
    33001 gcaatccagc tggcgacaat cttttccccg aatagctcta ttccgcttga gaagagaaca
    33061 aggctgaggc caaacagcaa aaagccagtg gtatcgaatc tgcgtcgtgc ggtggtgaaa
    33121 ttgggcatat gtttgcgcgc gtaaagaagg cccgcaatac ctatggggat attgattaaa
    33181 aatatccagt gccaggttgc ccaggtgacc agcacgccgc caagaacggg gcctaaaatt
    33241 ggccccacca gacccggcat ggcgacaaaa ttcaatacag gaagaagttc attacgagga
    33301 taagcgcgca gtaaggccag ccgagcaaca ggcatcatca ttgcgccgcc tatcccctga
    33361 ataacccgga agacaaccag ctgtggtagc gagttggaaa gtgcgcaggc cagagaaccc
    33421 aacgtgaaca gactcacggc aagggtaaaa atgcgacgtg taccgaagcg atcggctagc
    33481 catccgctta ccggaataag catcgccacc gtcagcgtat aactgatgat ggctgattgc
    33541 atcgcgagag gagaacgatt aaggctatga gcgattgcgg gtaaggcggt attcagaata
    33601 gtggcatcaa gtgcctgcat gaagaaggcc atcgccgcga tccacggcaa acccgccata
    33661 ctgcgcttct ttttatcgct cattcaatgt cctgttatcg ggttatcact tatctggtga
    33721 gcgtagcagc gcctgacaag ctttaaatgc cgcgtcgcca tcgctttgga taatcgcatc
    33781 gacaatcgcc tggtgcagat ccagctttat cactgtgtcg ctggtaattg acgtgaagta
    33841 agtgtgataa accgaatgga atagcgaggc gaatgaggtc aaaaacggat tggcgctcat
    33901 ttcatagata tgctcatgcc aggccatatc gacttcgatc cagcgttcac ggcgaaagtt
    33961 ctcttttaat gccgccattt cggccattaa cgtattgaga tgcgccttct gttccgcggt
    34021 gccaaccttt gctgccagta ggcaagcttg cggctccaaa cagatacgta taaccagaaa
    34081 gtgatcgatg acctgatgaa agttctcttc tgtcatccac caggtaagca attcctgatc
    34141 aagaaaattc cagttcgatt gtggcatgac tcgggtacca attcgcggtc gcggtaaaac
    34201 catccctttt gccgttaacg ttttgatcgc ttcgcgtacc gctgtacgac tcactccaaa
    34261 ttgctcgccc agctcaatct caccaggcaa aatggtgccg ggttcatatt caccttttaa
    34321 gatccgctgc gccagcttct cagccagaac atacgaaagg tttttctgtg cagctaactg
    34381 ttgtgcgctt aaaggcattg ctaatcttcc tttttctttt tattccttct tagtatgcca
    34441 ccaggaagtg tgattacggt tgcaaaaacg gcaaattgat tgttttatgg cacattaacg
    34501 gggcttttgc tgaaaaaatg cgcggtc
//
LOCUS       DAZFFE010000039        34242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFFE010000039 DAZFFE010000000
VERSION     DAZFFE010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.039"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            41..2467
                     /gene="ynfE"
                     /locus_tag="ACHJ69_002105"
     CDS             41..2467
                     /gene="ynfE"
                     /locus_tag="ACHJ69_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFO5015093.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAEAAV
                     QQANEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVKQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPTYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTRELEKDEVISPLPVYASTFEGWDSPER
                     SAFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            2528..4951
                     /gene="ynfF"
                     /locus_tag="ACHJ69_002106"
     CDS             2528..4951
                     /gene="ynfF"
                     /locus_tag="ACHJ69_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO5015094.1"
                     /translation="MKIHNTEALMSAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAQAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVQACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAP
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FRVDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            4962..5579
                     /gene="ynfG"
                     /locus_tag="ACHJ69_002107"
     CDS             4962..5579
                     /gene="ynfG"
                     /locus_tag="ACHJ69_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO5015095.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            5581..6435
                     /gene="ynfH"
                     /locus_tag="ACHJ69_002108"
     CDS             5581..6435
                     /gene="ynfH"
                     /locus_tag="ACHJ69_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO5015096.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHII
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAATILRAARVTFNTTPFAIISILALIACAGLIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVMGLILILGGEMIGRVLFYCLHM
                     TVGMAIAG"
     gene            6478..7092
                     /gene="dmsD"
                     /locus_tag="ACHJ69_002109"
     CDS             6478..7092
                     /gene="dmsD"
                     /locus_tag="ACHJ69_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO5015097.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTRDGWET
                     QWPLPEASLAPLVTAFQTQSEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            7252..8544
                     /gene="clcB"
                     /locus_tag="ACHJ69_002110"
     CDS             7252..8544
                     /gene="clcB"
                     /locus_tag="ACHJ69_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO5015098.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATIVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLISNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLFAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(8497..9192)
                     /gene="bioD"
                     /locus_tag="ACHJ69_002111"
     CDS             complement(8497..9192)
                     /gene="bioD"
                     /locus_tag="ACHJ69_002111"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO5015099.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(9317..10537)
                     /gene="mlc"
                     /locus_tag="ACHJ69_002112"
                     /gene_synonym="dgsA"
     CDS             complement(9317..10537)
                     /gene="mlc"
                     /locus_tag="ACHJ69_002112"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO5015100.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(10672..11565)
                     /gene="ynfL"
                     /locus_tag="ACHJ69_002113"
     CDS             complement(10672..11565)
                     /gene="ynfL"
                     /locus_tag="ACHJ69_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5015101.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTEAGKQFLVDSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVTIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            11672..12925
                     /gene="ynfM"
                     /locus_tag="ACHJ69_002114"
     CDS             11672..12925
                     /gene="ynfM"
                     /locus_tag="ACHJ69_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5015102.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHDILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHHRLHA"
     gene            complement(12975..13094)
                     /locus_tag="ACHJ69_002115"
     CDS             complement(12975..13094)
                     /locus_tag="ACHJ69_002115"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO5015103.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            13350..13658
                     /gene="asr"
                     /locus_tag="ACHJ69_002116"
     CDS             13350..13658
                     /gene="asr"
                     /locus_tag="ACHJ69_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO5015104.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            13751..13834
                     /locus_tag="ACHJ69_002117"
     CDS             13751..13834
                     /locus_tag="ACHJ69_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015105.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            13934..14755
                     /gene="ydgD"
                     /locus_tag="ACHJ69_002118"
     CDS             13934..14755
                     /gene="ydgD"
                     /locus_tag="ACHJ69_002118"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO5015106.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(14794..15123)
                     /gene="mdtI"
                     /locus_tag="ACHJ69_002119"
     CDS             complement(14794..15123)
                     /gene="mdtI"
                     /locus_tag="ACHJ69_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO5015107.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(15110..15475)
                     /gene="mdtJ"
                     /locus_tag="ACHJ69_002120"
     CDS             complement(15110..15475)
                     /gene="mdtJ"
                     /locus_tag="ACHJ69_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO5015108.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            15887..16921
                     /gene="tqsA"
                     /locus_tag="ACHJ69_002121"
     CDS             15887..16921
                     /gene="tqsA"
                     /locus_tag="ACHJ69_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFO5015109.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGQVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTTGGQSI
                     AVLLSDLNKE"
     gene            complement(16946..18334)
                     /gene="pntB"
                     /locus_tag="ACHJ69_002122"
     CDS             complement(16946..18334)
                     /gene="pntB"
                     /locus_tag="ACHJ69_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO5015110.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALIVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(18345..19877)
                     /gene="pntA"
                     /locus_tag="ACHJ69_002123"
     CDS             complement(18345..19877)
                     /gene="pntA"
                     /locus_tag="ACHJ69_002123"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO5015111.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            20401..21345
                     /gene="ydgH"
                     /locus_tag="ACHJ69_002124"
     CDS             20401..21345
                     /gene="ydgH"
                     /locus_tag="ACHJ69_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO5015112.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            21531..22913
                     /gene="ydgI"
                     /locus_tag="ACHJ69_002125"
     CDS             21531..22913
                     /gene="ydgI"
                     /locus_tag="ACHJ69_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5015113.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAIMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVERPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            22950..23672
                     /gene="folM"
                     /locus_tag="ACHJ69_002126"
     CDS             22950..23672
                     /gene="folM"
                     /locus_tag="ACHJ69_002126"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO5015114.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(23669..24004)
                     /gene="ydgC"
                     /locus_tag="ACHJ69_002127"
     CDS             complement(23669..24004)
                     /gene="ydgC"
                     /locus_tag="ACHJ69_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO5015115.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            24133..24852
                     /gene="rstA"
                     /locus_tag="ACHJ69_002128"
     CDS             24133..24852
                     /gene="rstA"
                     /locus_tag="ACHJ69_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO5015116.1"
                     /translation="MNTIVFVEDDAEVGALIAAYLAKHDMQVTVEPRGDRAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            24856..26157
                     /gene="rstB"
                     /locus_tag="ACHJ69_002129"
     CDS             24856..26157
                     /gene="rstB"
                     /locus_tag="ACHJ69_002129"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO5015117.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            26233..27162
                     /gene="tus"
                     /locus_tag="ACHJ69_002130"
     CDS             26233..27162
                     /gene="tus"
                     /locus_tag="ACHJ69_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO5015118.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNFSQAALVSHIQHINKLKTTFEHIVTVESELPSAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(27159..28562)
                     /gene="fumC"
                     /locus_tag="ACHJ69_002131"
     CDS             complement(27159..28562)
                     /gene="fumC"
                     /locus_tag="ACHJ69_002131"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO5015119.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DRWVRPEQMVGSMKAGG"
     gene            complement(28705..30351)
                     /gene="fumB"
                     /locus_tag="ACHJ69_002132"
     CDS             complement(28705..30351)
                     /gene="fumB"
                     /locus_tag="ACHJ69_002132"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO5015120.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            30550..31725
                     /gene="manA"
                     /locus_tag="ACHJ69_002133"
     CDS             30550..31725
                     /gene="manA"
                     /locus_tag="ACHJ69_002133"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO5015121.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVHDAAGDIVSLRDVIESDKATLLGDAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVRGHGRLARVYNKL"
     gene            31826..33334
                     /gene="ydgA"
                     /locus_tag="ACHJ69_002134"
     CDS             31826..33334
                     /gene="ydgA"
                     /locus_tag="ACHJ69_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO5015122.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKVNPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTVNSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(33379..>34242)
                     /gene="uidC"
                     /locus_tag="ACHJ69_002135"
     CDS             complement(33379..>34242)
                     /gene="uidC"
                     /locus_tag="ACHJ69_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO5015123.1"
                     /translation="SLPYRIPLMAGSSQRTLPTVSEGALGYWALTPNIDLWGMWRSRV
                     FLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDTSRYSLGASVQKDVSNQIQ
                     SILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETALVSGQLTWIAPWGSVFGS
                     GGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVNYRLTPQFTLTFAPIVTRG
                     YESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKGREKRDGSALGDRLNYWDV
                     KMSIQYDFMLK"
BASE COUNT         8419 a         8819 c         8433 g         8571 t
ORIGIN      
        1 tataaacatt tatataacga taaagaacag ggagtgagtt atgtccaaaa atgaacaaat
       61 ggtgggcatc agccgcagaa cactcgttaa atctaccgcg ataggttctc tggcgctggc
      121 tgcaggcggt ttttctttgc cgtttaccct gcgcagtgca gaagcagcgg tacaacaggc
      181 caacgaaaaa gtggtctggg gtgcctgttc cgtcaactgt ggtagccgct gtgcacttcg
      241 tctgcatgtt aaagataatg aagtgacctg ggtggaaact gacaacaccg gcagcgacga
      301 gtatggcaac catcaggtac gcgcctgttt gcgcggtcgc tccatccgtc ggcgtattaa
      361 tcatcccgat cgcctgaatt acccaatgaa acgcgtgggc aaacgcggcg aaggcaaatt
      421 cgaacggatt agctgggatg aagccctgga tactatcgcc agtagcctga aaaaaaccgt
      481 caaacaatat ggcaatgagg ctgtatatat tcagtactct tcggggattg ttggcggcaa
      541 tatgacccgc tcttctccat cagcctcggc ggtcaaacgc ctgatgaact gctacggcgg
      601 ttcactaaat cagtatggct cctacagcac tgcgcaaatt tcctgtgcca tgccctacac
      661 ctacggcagt aatgatggca acagcaccac ggatattgaa aacagcaagc tggtcgtgat
      721 gtttggtaac aacccggctg aaacccgcat gagcggcggt ggcatcactt atcttcttga
      781 aaaagcacgc gagaaatcga acgcaaaaat gattgttatc gatccgcgat ataccgatac
      841 ggctgctggt cgtgaagatg aatggcttcc tattcgcccg ggcaccgatg ccgcgctggt
      901 tgcgggtatt gcctgggtat tgattaacga aaatctcgtt gatcaacctt ttctcgataa
      961 atactgcgtc ggctatgacg aaaaaacctt acccgcagat gcacccaaaa atggtcacta
     1021 taaagcctat attcttggtg aaggcgatga taaaacagcg aaaacgccgc agtgggcttc
     1081 gcaaattaca ggtatcccgg tagaccgtat catcaaactg gcgcgtgaaa ttggcacagc
     1141 aaaacccact tatatctgcc agggctgggg gccgcaacgc caggcaaacg gcgagctgac
     1201 ggcacgcgct attgctatgt tgcctatttt gacgggcaat gtcggcatca gcggcggaaa
     1261 tagtggtgcg cgtgaatcga cctacaccat taccatcgaa cgcttgccgg tgctggataa
     1321 tccggtcaaa acgtcaatct cctgcttcag ctggacagat gctatcgatc atggcccgca
     1381 aatgacggca atccgcgacg gcgtccgggg caaagataaa ctagatgtgc ccattaagtt
     1441 catctggaac tacgcgggaa ataccctggt taatcagcat tctgacatca acaaaacgca
     1501 tgaaattctg caggacgaat cgaaatgcga aatgattgtg gtcatcgaaa actttatgac
     1561 ctcatcggca aaatatgccg acattctgct gccagacctg atgaccgttg agcaggaaga
     1621 tattattcct aacgactacg ccggtaacat gggatatctc attttcctcc agcctgtcac
     1681 cagcgaaaaa ttcgaacgca aaccaattta ctggatcctg agtgaagtcg cgaaacgtct
     1741 tggacctgac gtctatcaaa agttcactga aggtcgcacg caggaacaat ggttacaaca
     1801 tctgtacgcc aaaatgcttg ccaaagatcc ggcgttaccg tcttacgacg aattaaaaaa
     1861 aatgggtatc tataagcgta aagatcccaa tggtcatttt gtcgcctaca aagcatttcg
     1921 tgacgacccc gaggcaaatc cacttaaaac gccttccggt aagattgaaa tttattccag
     1981 caagctggcg gaaattgccc gtacccggga actggaaaaa gatgaagtga taagcccact
     2041 gccggtttat gcctcaacct ttgaaggctg ggactcccct gaacgtagtg ccttcccact
     2101 gcaactgttt ggtttccatt acaaatcccg tacgcactcg acctacggca acattgatgt
     2161 cctgaaggca gcctgccgtc aggaagtgtg gatcaaccct atagatgcac agaaacgtgg
     2221 gattgccaac ggcgatatgg tgcgggtgtt taaccatcgt ggcgaagttc ggctaccagc
     2281 caaagtgaca ccacgcattc tccctggagt tagtgctatg ggccagggag cctggcacga
     2341 ggccaatatg tctggcgata aaatcgacca cggcggctgt gtgaatacgc taaccactct
     2401 gcgcccttca ccactggcga agggaaaccc gcagcacact aatctggtcg agatcgaaaa
     2461 aatataaccc actacgacca taaattttga catgacattt tgtttgaaaa gcaataagtg
     2521 agtaatgatg aaaatccata acacagaggc gctcatgtcg gctgaaatca gtcgtagaag
     2581 tctgatgaaa acctccgcac ttggcagtct cgcgctggca agcagtgctt tcactttgcc
     2641 gttttcccaa atggtccgag cggcacaggc accggtagaa gagaaagcgg tctggagttc
     2701 ctgcaccgtt aactgcggga gccgctgtct gttacgtttg catgtgaaag atgacaccgt
     2761 gtactgggtg gagtctgata cgacaggtga cgacgtttac ggtaatcatc aggttcaggc
     2821 atgtttacgc gggcgctcta tccgccgacg gatgaatcat cctgacaggt tgaaatatcc
     2881 catgaagcgc gtcggcaagc gcggtgaagg taaatttgaa cggataagtt gggacgaagc
     2941 cctggatacc atcagtgata atcttcggcg gatcctaaaa gattacggca atgaggctgt
     3001 acatgttctc tatggaacag gcgtagatgg cggaaacatc accaactcaa acgtcccgta
     3061 ccgtctgatg aactcttgcg gtggttttct cagtcgctat ggcagctaca gtaccgcaca
     3121 gatcagtgcc gcaatgagtt atatgttcgg tgccaatgat ggcaacagcc cggatgatat
     3181 cgccaatacg aaactggtcg ttatgttcgg aaataacccg gcagaaacac ggatgagcgg
     3241 cggtggtgtc acttactacg tcgagcaagc ccgcgaacgt tcaaacgcac gcatgatcgt
     3301 catcgatcca cgttataacg acacagctgc cgggcgtgaa gatgaatggc tgcccattcg
     3361 ccctggcacc gatggcgcac tggcctgtgc aattgcctgg gtactgatta ctgaaaacat
     3421 ggtcgatcag ccatttctcg acaaatattg tgttggttac gatgaaaaaa cgctgcccgc
     3481 caacgcacca cgtaacgcgc attataaagc ctatattctg ggcgaagggc ctgacggcat
     3541 agctaaaacg ccggaatggg cagcaaaaat caccagcatc ccggcagaaa aaattatcca
     3601 gttggcacga gagatcggtt cagcaaaacc ggcttatatt tgtcaggggt gggggccaca
     3661 acgacattcc aacggcgagc aaacgtcccg cgctattgcc atgctttccg ttctcaccgg
     3721 taacgtcggc ataaacggcg gcaactcagg tgtacgcgaa ggtagctggg atctgggggt
     3781 agaatggttc ccgatgctcg agaatcctgt taaaacgcag atttccgtct ttacatggac
     3841 agatgctatc gaccatggta cggaaatgac cgcgccccgt gatggtgttc gtggaaaaga
     3901 aaaactggat gtccccatca agtttttatg gtgctacgcc agtaacacat tgatcaatca
     3961 acatggcgac atcaatcaca cccatgaggt gcttcaggat gacagcaagt gcgagatgat
     4021 tgttggcatt gaccacttca tgactgcctc ggctaagtat tgcgatatcc tgttacccga
     4081 cctgatgcca acagagcaag aagacctcat ctctcatgag tctgccggga atatgggcta
     4141 tgtgatcctc gcccaacccg caacctcagc aaaatttgaa cgcaaaccca tctactggat
     4201 gctgagtgaa gtcgccaaac gcttaggacc agacgtttat caaaccttta ctgaaggtcg
     4261 cagtcagcat gaatggatca aatatctcca tgcgaaaacg aaggaacgta accctgagat
     4321 gcccgactac gaggagatga aaacgaccgg gatctttaag aaaaaatgcc cggaagaaca
     4381 ctacgtcgct ttccgcgcat tccgtgtaga tccacaggca aacccattga aaacaccttc
     4441 gggaaaaatc gaaatttatt ctgaacgact ggcgaagatt gcggatacct gggaattgaa
     4501 aaaagatgaa attattcatc ctcttccagc ttatacgcca ggttttgatg gctgggacga
     4561 tcccctgcgg aaaacttatc cactgcagtt aacgggcttc cactacaaag cgcgtaccca
     4621 ctccagttac ggcaatattg atgtattaca gcaggcctgc ccacaagagg tgtggatcaa
     4681 ccccattgat gctcaggcac gcggtatccg tcatggcgat accgtgcggg tatttaacaa
     4741 taatggagaa atgctgattg ccgcaaaagt gactccgcgt attctgcctg gcgttaccgc
     4801 catcgggcaa ggtgcttggc ttaaagcgga tatgtttggt gaccgggtcg atcacggcgg
     4861 cagtatcaat attctgacct ctcaccgccc ttcaccgctg gcaaagggaa acccgtcgca
     4921 cagcaatctt gtccagatcg aaaaggttta aggagtagcc catgaccaca caatatggat
     4981 tttttatcga ttccagccgc tgtactggct gtaaaacctg cgaactggcg tgcaaagatt
     5041 ttaaagatct tggcccggaa gtcagtttcc gccgtattta tgaatatgct ggcggcgact
     5101 ggcaggaaga taatggcgtc tggcaccaga acgtgttcgc ttattatctc tccatctcct
     5161 gtaaccattg cgatgacccc gcatgtacaa aagtctgccc cagcggtgca atgcataagc
     5221 gtgaagatgg tttcgtggtg gttgatgaag atgtttgtat cggctgccgc tactgccaca
     5281 tggcttgtcc gtacggcgcg ccacagtaca atgctgaaaa agggcacatg acgaagtgcg
     5341 atggttgtta ttcgcgcgtc gccgagggga aacaacccat atgtgtcgaa tcctgcccac
     5401 tgcgtgcact tgagttcggt ccaattgaag aattgcgcca gaagcacggc actctggctg
     5461 ccgtcgcgcc actgccgcgc gcgcatttca caaaacccaa tatcgttatc aaacctaacg
     5521 ccaacagccg cccgaccggc gataccactg gttatctggc taatccggag gaagtgtaaa
     5581 atgggaaatg gatggcatga atggccactg gttatcttta ccgtattggg tcaatgcgta
     5641 gtaggcgcgc tgattgttag cggaattggc tggttcgccg ccaaaaatga cgcagaccgt
     5701 cagcacatta ttcgtggcat gtttttcctc tggctgttga tgggcatcgg gtttattgcc
     5761 tctgtcatgc acctgggatc gccactgcgg gcgtttaact cgctgaatag aatcggagct
     5821 tctggtctga gtaacgagat tgctgccggt tccatatttt ttgccgttgg cggattatgg
     5881 tggctggtgg cggtcatcgg taaaatgccg caagcgttgg ggaaactctg gttacttgtc
     5941 agcatggcgc tcggcgtcat tttcgtctgg atgatgacct gcgtgtatca aatcgacact
     6001 gtgccaacct ggcataacgg ttacactacg ctggctttct tcctgacggt attgttgagc
     6061 ggtccaatac ttgctgccac aattctacgt gcagcccgcg ttacatttaa taccacacca
     6121 tttgccatca ttagcattct ggcgctgatc gcttgtgccg ggttgattgt gctgcaaggt
     6181 ttgtctctgg cgtctattca ctcatccgta caacaagcca gcgcactggt accagattac
     6241 gcctccttac aggtctggcg tgtggtattg ttatgcgcag gtcttggatg ctggctttgt
     6301 ccgctcattc gtcgccgtga acctcacgtt gccggacttg ttatggggtt aatcctcatt
     6361 cttggcggtg agatgatcgg gcgtgtgctc ttttattgcc tgcacatgac ggtaggaatg
     6421 gctattgcag gttaacaaat tggtgcgggg caacccgcac atttcaggat gttaggaatg
     6481 acccattttt cacagcaaga taatttttct gttgcggcgc gcgtgttggg cgcgctgttt
     6541 tattacgctc cagagagtgc agaagccgca cctctggttg cggtactcac ccgtgacggc
     6601 tgggaaactc agtggcctct accagaagcg tcattagcgc cgctggtgac ggcttttcag
     6661 acacagagtg aagagactca cgcccaggcc tggcagcgtt tgtttgtcgg cccgtgggca
     6721 ctgccgtctc cgccgtgggg atcggtctgg ctggatcgcg aatctgtgct gtttggcgat
     6781 tcaacattgg cacttcgtca gtggatgcgc gagaaaggca ttcagttcga aatgaagcaa
     6841 aacgaaccgg aagatcattt tggttcactg ctgctgatgg ctgcgtggtt ggcggagaat
     6901 ggtcgccaga cagaatgcga agaattgctg gcatggcacc tttttccgtg gtcaacacgt
     6961 tttcttgacg tttttatcga aaaagcagaa caccctttct accgtgcact gggtgaactc
     7021 gctcgcttaa cgctggcgca atggcaatca caactgttga ttcctgtcgc ggttaaaccg
     7081 ctgtttcgat aggatcaccg tattattgcc ggatggtgac gattacacgc aattcggcaa
     7141 ttaataggtt acagtgtcac gtttttttat ctcttaaagc acgcactgct tttgcggctg
     7201 gcctcttttg ccgcaaaata gtcgcccgtg tttcattgcc catttctgct catgcatcat
     7261 ctacacatct atccggatct gcgcactatg ttccgccgtc tgcttatcgc aacaatcgtc
     7321 ggtattcttg cggcctttgc cgttgccggg tttcgtcatg cgatgctgct actggagtgg
     7381 ctgttcctca ataatgactc cggcagtctg gtcaatgcag cgacaaatct ttccccgtgg
     7441 cgacggctgc taaccccggc gctcggcgga ctggcggcgg gtttgttgct gatgggctgg
     7501 cagaaattta cccagcaacg ccctcatgcg ccgaccgatt acatggaagc gttgcaaacc
     7561 gatggtcagt ttgattacgc agcaagcctg gttaaatcgc ttgcctctct actggtagta
     7621 accagcggca gtgcaattgg tcgcgaaggt gcgatgattc ttttagctgc ccttgcagcc
     7681 tcgtgttttg cccaacgttt tacgccacgc caggagtgga aattatggat cgcctgtgga
     7741 gctgcggcgg gaatggctgc ggcctatcgc gccccgcttg ctggcagttt atttatagcc
     7801 gaagtgctgt ttggcactat gatgttggcc tctctcggcc cggtgattat ttccgccgtc
     7861 gtggcgttgc tgattagcaa tcttattaat catagcgacg cattactcta cagcgtacaa
     7921 ctctcagtga cggttcaggc tcgtgactat gcgctgatta tcagtacagg tgtgctggca
     7981 ggtctgtgcg gaccactgtt gttaacgtta atgaacgcct gtcatcgtgg atttgtaagt
     8041 ctcaaacttg cgccgccctg gcaactggca ctgggcgggt tgatcgtggg tctgctttcc
     8101 ctgttcacac ctgcagtgtg gggcaacggc tatagcaccg tacaatcctt tttaaccgcc
     8161 ccgccactgt taatgatcat tgccgggatc ttcctctgta aactgtttgc cgtgctggcg
     8221 agtagtggtt ctggcgcgcc cggtggtgtc tttacaccga cgctatttat cggtcttgcc
     8281 attggcatgt tgtatggtcg tagcctggga ttatggttcc ctgatggcga agaaattacg
     8341 cttttactcg gattgaccgg gatggcgaca ctgttggcgg cgaccacgca cgcgccgatt
     8401 atgtcgacgt tgatgatatg tgaaatgacc ggggagtatc agctactccc cggtttattg
     8461 attgcctgcg taattgcgtc ggtaatttcg cggacgttac accgtgactc tatctaccgc
     8521 cagcacactg cgaagcatag ctaagcggat gtattgcccc agttcacgct gttcggcgcg
     8581 cggcagataa ggcagttcac caatgagcgg tgccggaagt tttttaccga gcacatcaat
     8641 gatttccgca taatgcgcca gtcctggatt gattcggtta gccacccagc caatgagcgg
     8701 tagcccgtcg ttggcgatcg cctgagctgt tagcagtgca tggttaatgc aaccttcctg
     8761 aataccgaca accatcaaca ccggcagttg ttcctgcacc acccattcag agagcggacg
     8821 caaatcattc atcagactgc gccagccgcc agtcccttct accaccacat gatcgacttt
     8881 atcggtcagg tttgccaggc cgtttgaaat gagggtgtaa ttgattgggc aactgtgcgc
     8941 cacgctactt tcttcttcgc ttaacgcgat aggattaact gcttcataag gcagttcgat
     9001 ggttgaaaca ctctgcaaca ccagggcatc tttattacgc agcccttcgg gtgtctcttt
     9061 gctccccttc gctacgggtt tatatcccgc aaccgttttt ccctgggagg ctaacgcttg
     9121 tagcaatgcg cgggaaacca ccgttttccc tacagaagtg tctgtaccgg taataaagaa
     9181 acgcttcagc atcactaact ccaccgttat gcttcacaaa tataaactag gaaaataatt
     9241 aaccttgaaa gtctaagtta tgctttcctg gcccaaattg agatagcgca aattttggta
     9301 gaacagttaa aaaatgttaa ccctgcaaca gacgaatcaa caaagaaccg ttatacatcg
     9361 cgtcttttac tagcgcagcc cctgccatcg taccctggtt agaaaattga gtactctcaa
     9421 cgctaatgtg ctgactatac gcaggaaggg cctgctgacg gatgctgtcc gagatgaccg
     9481 ggaagaggat atctgccgct ttacttaacg gtgagccaat cagtattttt tgtgggttaa
     9541 ataaattcac catgatggca agaatgcgcc cgacatgcgc gcccaccccg gtaatgatgt
     9601 cttttgccag tagatcgccg cgcaatgccg cctgacacaa tgagtccacg gttaacggct
     9661 gtccatgtaa catcgagctc atggattgat tgagacgcag ctgtgccagc tcaagaatac
     9721 tgtccacact ggcgatggtt tcgaggcagc cgtgattccc gcaataacag cgtttcccat
     9781 acgggtcgac ctgcgtgtga cctatttcca cgaggctact gctgccggcg tgtagcagat
     9841 gaccatcggt aatgacgccc gcccccacgt tgtgatcgat aaccacctga atcacatcgc
     9901 gcgccccgcg tgaggcacca aacaaggcct ctgccatcgt ccatgcgctg atatcatgct
     9961 ggatataaac cggtacgccg gtatgctgct ccagcgcctc gccgagcggc atctctttta
    10021 catcctcgta gaacggcatg cgatgtacaa tgccattttc cgtatcaata attcccggca
    10081 aggttatggc aatcgaagtc agacgctcaa gttttttctg gtggcggata aaaaactgat
    10141 cgatatggga aatgatacga tccagcaatg gcgagtcatc ttttaacgcc agttcctgcg
    10201 actcttccac caccagtttg ctgctcagat cgcgcagagc aaggaaaatc tccccgcgac
    10261 taatgcgcag agaaagatag tgccaggctt cagtttcaac caccagcccc accgccggac
    10321 ggccacggtt ccccgcttct ttgatttcca gctcttgcac caggtgtgct tcgagcatct
    10381 cacggacaat tttagtgata ctggcaggag ccagttgcgc cagacgggaa agatcgatac
    10441 gcgagactgg accaagctga tcaatcaggc gataaaccgc gcctgcgttg gtctgcttta
    10501 tttgatcaat gtgcccaggc tgattttcag caaccaccgc atactcccta tattttcgcg
    10561 ctccgaaata atctgtaggc tatggtgaag cacttcaata cgtgtcgtca aatttttact
    10621 taggcatgtg attaacagca catttttcgg gctttttcgc tgaaatttcc ctcacctgag
    10681 agcattcagc agatgaatac gaaagtttcg cgcagctgga ctttgttcat gatgtttcgg
    10741 ccagaccaac cacatttcag aaaccgcatc ctcttcagca atcgtcaccc agcgcatttc
    10801 gttgagctga acacgtttaa atgacgcagg caaaattgaa acacccagac cggcggaaac
    10861 cagaccgatg atggtcattg cctcacccac ctcctgagtg atgacgggcg tcaaattgta
    10921 acgtctcatc agcccgagaa tatcgtcata cagccctgtt ccgacgtgcg gatcaaaaaa
    10981 gacaaagggt tctttcgcca gttcagccag cgttacactc gggttatttg ccaggggatg
    11041 atcgtgcggg atcatcgcca taagcggttc atggacgatg actgcgtgtt caagcgtctc
    11101 cggtaacgct gtgttacgca gcaatcccat atccagcgtt ccttcaatga gcggagcgat
    11161 ttgctcgcga gtgttcattt cgcgggtttg taaatggaca tcgggataat cacggcgaaa
    11221 cagcgataac gtatcggaca ccgcccgaat aaaaggagcg gacgaagtaa aaccaatgcg
    11281 caactccccc gcttcgccct gatgcagcct ttcagcgcga gcggcagcat catccaccat
    11341 agacaggatt tgccgactat ctacaagaaa ctgttttcct gcttccgtca gcaatacact
    11401 gcgattggtc cgtgccagca gtcgggcacc aatttgttgc tccagcgcct gaatctgctg
    11461 acttagaggt ggttgcgaaa tattcaggcg ggcagcggca cgcccgaaat gcagctcttc
    11521 tgcaacagcg acaaagtaac gcagatgacg aagttcaata ttcatattta aagcatctta
    11581 tttgagatta ttaatatatt agacagaaca attcgatttt cctaatctat tcataagcct
    11641 gatccacagg catatttagc aaggatttca agtgagccgt actacaactg ttgatggcgc
    11701 tccggcaagc gacactgaca agcaaagcat ttctcagcca aatcaattta ttaaacgcgg
    11761 tactccgcaa tttatgcgcg tcaccctggc gcttttctct gccggactgg caaccttcgc
    11821 acttctctat tgtgtgcaac ctatccttcc ggtgctttcg caggagtttg gcttaacacc
    11881 cgcgaacagt agtatttcac tgtccatttc aacggcgatg ttggctatcg gtttgctgtt
    11941 tactggcccg ctatccgatg ccattggtcg caaaccagtg atggtcacgg cgctactgtt
    12001 ggcctccatt tgtacgttac tttcgacaat gatgaccagc tggcacgaca ttttgattat
    12061 gcgcgccttg attgggcttt cgttaagtgg ggtggcagcc gttggcatga cttatcttag
    12121 cgaggaaatc caccccagtt tcgtggcctt ttcaatgggg ttgtatatca gcggcaactc
    12181 aattggcggc atgagcggac gcttaattag cggtgtcttc acggactttt tcaactggcg
    12241 aattgctctg gcggcaatcg gttgtttcgc gctggcctcg gcgttgatgt tctggaaaat
    12301 cctccctgaa tcacgccatt ttcgcccgac ttctctgcgc cctaagacgt tatttatcaa
    12361 ctttcgtctg cactggcgtg accggggatt accgttattg ttcgcagaag gctttttgct
    12421 gatggggtcg ttcgtcacgc tgtttaatta catcggctat aggttgatgc tttccccctg
    12481 gcatgtcagt caggccgtgg ttggcttatt atcgctggct tatttgaccg gtacatggag
    12541 ctcacccaaa gccggaacca tgaccacccg ctatggacgc ggtccggtga tgttgttttc
    12601 gacgggggtt atgctgtttg gtttactgat gaccttattc agctcgctgt ggctgatctt
    12661 tgccggaatg ttactcttct cagccggatt cttcgcagcc cactccgttg ccagcagctg
    12721 gatcggcccc cgcgcaaaac gcgctaaagg ccaggcctcc tcgctgtatc tgttcagtta
    12781 ctatctgggg tcgagtattg ccgggacgct gggtggtgtt ttctggcata actatggctg
    12841 gaacggcgtc ggcgcattta ttgctctgat gctggtcatt gctctgctgg tcgggacgcg
    12901 tttgcatcat cgtctgcacg cctgaaaaaa taagtccgga ctgaggtaaa tccccgtccg
    12961 gacttattgc ctgctcaaac caacgttaat agccatccta aaatagacga agcgccagcc
    13021 aattcccgca gcgcatctag cgtcatcagg attataagta cccaaataaa cggattcatt
    13081 ttgctgtgtg tcatttatta ctgatgcgca gttattctac cgctttgtaa gtagtaaaat
    13141 agttaacacg atcaagacta ctattattgg tagctaaatt ttccttaagt cacaatacgt
    13201 tattatcaat gctgtaatct attcagcgtt tgtacatatc gttacacgct gaaaccaacc
    13261 actcacggaa gtctgccatt cccagggata tagttatttc aacggccccg cagtggggtt
    13321 aaatgaaaaa acaaattgag ggtatgacaa tgaaaaaagt attagctctg gttgttgccg
    13381 ctgctatggg tctgtcttct gctgcctttg ctgcagagac tgcgaccaca cctgctccga
    13441 ctgcgacgac caccaaagca gcgcctgcga aaaccacaca tcataaaaaa cagcataaag
    13501 cagcacctgc ccagaaagcg caggcggcta aaaagcatca taaaaatgcc aaagctgaac
    13561 agaaagcccc tgaacaaaaa gcgcaggcgg cgaagaaaca cgccaagaaa cacagccatc
    13621 agcaaccggc aaaacctgct gcacaacccg cagcgtaagt tttcaacagt aatgctggcg
    13681 cgccccctcg cgcctgaaaa ttacggtgct aagcgggtaa cgtttagcac cgcctttagc
    13741 ggagggtagt atggtgggcc gttatcgctt tgagttcatt cttatcatcc ttattttatg
    13801 cgcactgatt gccgcccgtt tttatctttc ctgatcgtag ttatctgatt ttactcccac
    13861 tttcatcccg tcccgtctat agtatttatg agggtttgct tttaataatc ataattaccc
    13921 accagagtgt gatatgcgta caaccattgc tgtagtgttg ggtgcaatta gtttgacgtc
    13981 tgcttttgtg tttgcagata aaccagacgt tgccaaatcg gcaaacgatg aggtcagcac
    14041 cctgtttttt ggtcatgatg atcgtgtgcc agtgaatgac acgacccaat caccgtggga
    14101 tgcggttggc caactggaaa cggccagcgg caatttatgt acggcgacgc tgattgcacc
    14161 caatctggca ttaacggcag gacactgttt attgacgccg ccaaagggta aagccgataa
    14221 agcggtagcg ctgcgtttcg tgtcaaataa aggtctttgg cgttatgaga tccacgacat
    14281 agaaggccgc gttgatccga cactgggaaa gcggttaaaa gcagatgggg atggctggat
    14341 tgtacctccc gcagctgcgc cctgggactt cggtttgatt gtgctacgta atcccccttc
    14401 tggcattaca ccgttgccgt tatttgaggg agataaagcc gcgcttaccg ccgcattaaa
    14461 agcggctggt cgtaaagtga ctcaggcggg ctaccctgaa gatcatctcg atacgttgta
    14521 cagtcatcaa aactgtgaag tgactggctg ggcgcaaacg tcggtgatgt cgcatcagtg
    14581 cgataccttg ccgggtgaca gcggttcgcc tctgatgttg cataccgatg acggctggca
    14641 attaattggg gtgcaaagct cggctcctgc cgcgaaagat cgctggcgcg ccgataaccg
    14701 ggccatttct gttaccggtt ttcgcgacaa gctggatcaa ctgtcgcaaa aataatgttc
    14761 aaacgctgcc cgacagcgcg ggcagcgtgt tcatcaggca agtttcacca tgatcattcc
    14821 agccaacagc agcaccaggc caatccagcc tttacgattt aaccgttgac caaacaagat
    14881 ccaacctgcg gctaacgtgg cggcaatacc aaacccgccc cacaatgcat aagcgacaga
    14941 caagtcgatc cctttaacag cctgagaaag cgcactaaag gcagccagca ccgccgccag
    15001 agagagcaag ccaaatattt tgcgacgaaa gccgtcagaa aatttcaaaa agacgttagc
    15061 aacgatttcc agcacgattg ccaatgccag ccaggcggcg tgaacccatt caaactgcgc
    15121 catggttcac ctccagttca ggtttacgcg ctttacgggt acctgacttg atcaacacaa
    15181 tcccggccac cagggtggtt aacccggcaa ttttcatcag cgataaactt tcgtcgaata
    15241 acaaaacgct aaacaaggta ataaataaaa taccaatacc ttcccacagc gcataagcca
    15301 cgcctaaggc gattttttta acggcgaaag agagaaatat atacgacaga gaaatcatca
    15361 ccagcattaa aataaagccg ccatttccct cactgacgct cgcccatttc atggacagcg
    15421 taccggtaat ttctgtagca atagccagac ctaataaaat ccaataaata tacattgtcc
    15481 ttctcctgca agagaattat tttaattttc gcttaattca gcgaaaccaa agtaaagtgg
    15541 tcaagccccg cgcttagcgc aagctttggc agaagaaagg actaaagcgc gttgcgccag
    15601 tgctgctcac ctacgagcaa gatagtggat gaggtacgaa gatgggtaaa tgtagaaaat
    15661 aacgtcctga acaaattgtc cataatatta caattatccg cagtgttgct tctcgtcctc
    15721 gcggatgata attgtcctcg gtagttgaac acgcctgatt tgtatcatag cttaagaatt
    15781 aactcaaaat attttcactt ctttacctga gcggtttgat tttcgttatg atgacggagc
    15841 gaaaaagaca ttattattag caaaggaaga aaaaacgggg acaagcatgg caaagccgat
    15901 catcacgctc aatggcctaa aaatcgtcat tatgttggga atgctggtca ttattctctg
    15961 cggtatccgt tttgccgccg agatcatcgt gccgtttatt ctcgcattat ttattgctgt
    16021 tattcttaac ccgctggtgc aacacatggt ccgctggcgt gtgccgcgtg tactggcagt
    16081 gtcaattttg atgaccatca tcgtgatggc gatggtgttg ctgttagctt atctgggttc
    16141 cgcgctcaat gagttgacgc ggacgttacc gcaatatcgc aactctatta tgacgccgct
    16201 gcaagcgatt gaaccgttgt tgcaacgcgt agggattgat gtctcagttg accagctggc
    16261 gcattacatt gatccgaacg cggcgatgac gttgctcacc aacttattga cgcagttatc
    16321 taatgccatg tcatcaatct ttttattgct gctgacggtg ctgtttatgt tgctcgaagt
    16381 gccacaattg cccggaaaat ttcagcaaat gatggcgcgt ccggttgaag ggatggcggc
    16441 gattcagcgt gcgattgaca gtgtgtctca ttatctggtg ctgaaaacag ccatcagcat
    16501 catcaccggc caggtcgcct gggcgatgct cgccgcactc gatgttcgct tcgcttttgt
    16561 ctggggattg ctggcctttg cgcttaatta catcccgaat attggttcag ttctcgcggc
    16621 aatcccccct atcgctcagg tactggtgtt taatggcttc tacgaagcgt tgctggtgct
    16681 ggcgggatat ctgctaatta atctggtctt cggcaatatt ctggagccgc gcatcatggg
    16741 acgtgggctg gggctttcca cattggtggt atttttgtcg ttgatttttt ggggatggtt
    16801 gttaggaccg gtgggtatgc tgctttccgt gccgttaaca attattgtca aaattgcgct
    16861 tgaacaaaca acgggaggtc aaagcatcgc cgttctgtta agcgatctca acaaagagtg
    16921 acggcctccg cagaggccgt cagggttaca gagctttcag gattgcatcc acgctggctt
    16981 tggcgtcacc aaacagcatg tgggtgtttt ccttgaagaa cagcgggttt tgcacaccag
    17041 catagccagt gttcatcgaa cgtttaaaga caatcacgtt ctgcgctttc cacacttcca
    17101 gcacaggcat accagcaatc ggactcttcg gatcatcctg cgccgccgga ttaaccgtgt
    17161 cgttagcacc aatcaccagt acggtatcgg tatcagcaaa gtcatcgttg atctcgtcca
    17221 tttccagcac gatgtcatac ggtacttttg cttcagccag caatacgttc atatgtccag
    17281 gcaaacgccc cgcaaccggg tggataccga aacgcacatt aataccacga gcgcgcaatt
    17341 tctcggtaat ttcagcgaca ggatattgcg cctgcgcaac tgccatgccg taccccggag
    17401 taatgatcac tgaatgggag tttttcagca gttccgctgt ctcttctgca gtgatttcgc
    17461 ggtgctcgcc cacttcctga tcatcaccag tagaagagcc gtcggtgccg aaaccacccg
    17521 caataacgct gataaaggaa cggttcatcg ccttacacat aatgtaagaa aggatagccc
    17581 ccgaagaacc gaccagcgca ccggtcacaa tcagcaggtc gttgctgagc ataaagcccg
    17641 cagccgcagc cgcccagccg gagtacgagt tcagcatcga caccaccact ggcatatctg
    17701 caccaccgat ggaggcgact aaatgccagc cgaataccag cgcaatggcg gtcattatca
    17761 gcaatgccag cacttgcagg ccgacgctat ccgtgcgaac aaatacaatc agcagcagga
    17821 aggaaacgat cagagccgcc aggttcattt tgtgacggtt tggcagcatc aacggtttag
    17881 acgaaatctt gccacacagt ttgccgaacg ccaccaccga acccgtgaac gttaccgccc
    17941 cgatgaagat accgaggaac acttccgtca ggtgaatatt gaccagaatc ggtgccattc
    18001 ccgcgtcatg atgcagatag ctgttaaagc caaccagcac tgccgccaga cccacgaagc
    18061 tatgcaggat cgccaccagt tctggcattt cggtcatttc aacttttttc gccagccgga
    18121 taccaattgc cccaccaatg accatagcca gcaagatcca gccaacatta cccgtatccg
    18181 gtccaaaaat ggttgcgatt aacgcaatcg ccatcccggc gataccgaag ttgttaccct
    18241 ggcgagacgt ttcatgtttc gaaagaccgg ccagactgaa gataaacagg atcgcggcaa
    18301 caatgtatgc agctgtaact aatcctccag acatatgtta ccccttaatt tttgcggaac
    18361 attttcagca tgcgctgagt cacggtgaag ccaccgaaaa tattaatgct ggctataagc
    18421 accgcgataa aactaaggaa gctaacccag ccgccctggc caatctgcaa cagtgctccg
    18481 acaacaataa tccctgaaat cgcgttggtg accgacatca acggtgtatg caacgcgtgc
    18541 gatacattcc acaccacgta ataaccgaca acgcaggcca gcgcgaacac agtgaagtgt
    18601 ccaaggaact cttttggcgc aacgcttgcc atccagccga aaaggatgat tgccaacgcc
    18661 atcaacgcgt atttacgcca cggtgagcag gcacattttt cctcagtttt cacttccggt
    18721 gccgcttttt gtgccgcctg cggctgagct gatacctgaa tcggcggtgc cggccaggta
    18781 atttcgcccg cacggatcac ggtcacgcca cgaatcacca catcatcaaa atcaacagtg
    18841 atgttgccgt ctttctcttt gcacaacagt ttcagcagat taacgaggtt agtgccataa
    18901 agctgtgagg attgcgtcgg cagacggccc ggaagatcgg tgtaaccaat tactttgaca
    18961 ccattttccg tagtgaagat ttcacccggc acggtgtatt cacagttgcc gccgttttgg
    19021 gctgccaggt cgacaatcac actgcccgcc ttcatggagt caaccatttc acgggtaatt
    19081 agcttcggcg ctggtttgcc tggaataagc gcggtggtga caatgatatc gacctctttt
    19141 gcctgggcgg caaagagttc catttccgct ttgatgaacg cgtccgacat cactttggca
    19201 tagccatcgc cgctgcccgc ttcctcttta aaatccagct cgaggaattc tgcgcccata
    19261 ctttgaactt gttctttcac ttccgggcgg gtgtcgaatg cacgcacaat cgcgccgaga
    19321 ctgtttgctg cgccaatggc ggccagacct gcgacaccgg caccaatcac catcactttt
    19381 gccggtggca ctttcccggc cgcagtgatt tgtccggtaa agaagcgccc aaattcatgt
    19441 gcagcttcaa caatggcgcg ataaccggcg atattcgcca tcgagcttag tgcgtccagc
    19501 gattgtgcgc gtgagatacg cggcacagag tccatcgcca tcacggtcac gttacgttcc
    19561 gcaagttttt gcattaattc cggattctgc gcaggccaga taaaactcac cagcgttgtc
    19621 ccaggattca gtaacgcaat ttcatcatct aacggcgcat tgaccttcag aatgatctct
    19681 gactgccaga cgctattccc ttctacaatt tcagcgcccg cttgcacaaa cgctttatcg
    19741 tcaaaacttg ccagttgacc cgcgccgctc tctaccgcga cggtaaaacc cagtttcagc
    19801 agctgttcca ctgtttttgg cgttgctgca acacgggttt cattggttaa ccgttctctt
    19861 ggtatgccaa ttcgcatgat attcccttcc atcggtttta ttgatgatgg tttgcttgtg
    19921 tcaggagcca cacaagccgc tcatgtacga gctaaatgtt actccgttaa aataaattag
    19981 taacaaacgc cctataacgt actgaaaatt atgcctgtga tctagcgcca aaaatcagta
    20041 tttcggcgtg aactcgcaaa atattaacga ttcagccgtg atagtgggat aaacacctta
    20101 gaacgccgga taaagactga taattgtctt cggcggccgg gtaaaacgag acatcgcccc
    20161 ggtacaaatc actacttaac attaaattaa cttatacaat tcagttgctt cagtagtatt
    20221 gatggtgata cggctgtttt ttaagcatag acggtcattt gagcagaatt aaaattggtt
    20281 taaggaatgt gatatgaaaa atgacgcaga cagttacacc gtttaaatgc aataatcagc
    20341 cacgtttctc gttaataaca ataccagtac ctggtttgcg caaggcgaag gattattttt
    20401 atgaagctta agaacaccct cctggcgtcg gcactgcttt ctgctacggc attctccgtt
    20461 aacgcagcaa cagaactgac accggagcaa gcggcagcgg ttaaaccttt tgaccgtgta
    20521 gtggttaccg gtcgttttaa tgctattggc gaagcggtga aagccgtttc tcgtcgcgca
    20581 gataaagaag gtgccgcctc tttttatgtt gtcgacactt ctgattttgg taacagcggt
    20641 aactggcgtg tggtcgctga cctctataaa gccgatgctg aaaaagcaga agaaacaagt
    20701 aatcgcgtaa ttaacggtgt tgtcgaactg ccgaaagatc aggctgttct gattgaaccg
    20761 tttgacacgg tcaccgtcca gggcttctat cgtagccagc cagaagtcaa tgatgccatc
    20821 accaaagcgg caaaagcgaa aggtgcctac tctttctaca ttgttcgtca aatcgatgcc
    20881 aaccagggcg gtaaccagcg tattactgca ttcatctata aaaaagatgc taagaaacgt
    20941 atcgtccaga gcccggatgt aatcccggca gattccgaag caggacgtgc agctctggct
    21001 gccggtggcg aagccgcgaa gaaagttgag atcccgggtg ttgcgactac cgcatcacca
    21061 agttctgaag tcggtcgctt ctttgaaacc cagtcatcaa aaggcgggcg ttacaccgtc
    21121 acgctcccgg acggcactaa agtcgaagaa ctgaacaaag cgacagcagc gatgatggtc
    21181 ccgttcgaca gcatcaaatt ctctggcaac tacggcaaca tgaccgaagt ctcctatcag
    21241 gttgcgaaac gtgccgcgaa gaaaggtgct aagtattacc acatcacccg ccagtggcag
    21301 gaacgtggta ataacctgac cgtcagcgca gatctgtata aataactatt cagttactcc
    21361 tataaggcgg cttgatgccg cctttcgcat ttttagtcat aaatttccct gcgaccattg
    21421 catgcagaca catctcttcg taaaatcccg cgcttcagtg gcaaactcca tttttatgcg
    21481 ttttgcccta atatttattc ttcttgtcac gttttaatca ctggatatcg atggaaaaga
    21541 aactgggact gagcgcactc accgcgctgg tattaagctc aatgctgggc gcgggtgttt
    21601 tcagtctgcc gcaaaatatg gcggcagttg ccagcccggc agcactgctc atcggctggg
    21661 gtattactgg cgctggcatt ttattgctgg cctttgccat gctgatcctc acgcgcattc
    21721 gccccgaact tgacggcggt atctttacct atgcccgcga agggtttggc gagctaatcg
    21781 gcttttgttc cgcatgggga tactggctgt gcgcggtcat cgccaacgtc tcctatctgg
    21841 tgatcgtttt ttccgcgtta agctttttta cggacacgcc ggaattgcgc ctgtttggcg
    21901 atggtaatac ctggcaatcg atcgtcggtg catcggcttt attgtggatt gttcactttt
    21961 tgatcctgcg cggggtgcaa accgctgcca gcattaacct tgtggcgaca ctggcaaaat
    22021 tgttgccgtt gggtctgttt gttgtgctgg cgataatgat gttcaaactg gataccttca
    22081 agctcgactt caccggactt gcacttggcg tacccgtgtg ggaacaggtg aaaaacacca
    22141 tgctgatcac cctgtgggtt ttcattggtg tggaaggtgc cgtggttgtt tctgcgcgtg
    22201 cgcgtaataa gcgtgatgtt ggcaaagcga cactgctggc ggttctctcc gctctgggcg
    22261 tttatttgct ggtaacgctg ctttcgctgg gcgtggtgga acgtcctgaa ctggctgaaa
    22321 ttcgtaaccc gtcaatggcc ggtctgatgg tggaaatgat gggaccgtgg ggcgaaatca
    22381 tcatcgctgc cggtttgatc gtttccgttt gcggcgcgta cttaagctgg accatcatgg
    22441 cagcagaagt tccgttcctt gcagccactc ataaagcatt cccgcgcatt tttgcgcgcc
    22501 agaacgcaca agctgcgcca tccgcttcac tgtggctgac caacatctgc gtgcaaattt
    22561 gtctggtgct catctggctg accggttccg attacaacac attgctgacc atcgcctcag
    22621 aaatgattct ggtgccctat ttcctggtcg gcgcgttctt gctgaaaatc gcaacccgtc
    22681 cactgcataa ggcggtaggt gtcggtgcct gcatttatgg cttatggtta ctgtacgcct
    22741 ctggtccaat gcacttgctg ttatccgttg ttctgtatgc gccggggctg ctggttttcc
    22801 tgtacgcgcg taaaacgcat acacatgata atgtactgaa ccgtcaggaa atggtgctga
    22861 ttggcatgct gttaattgcc tcagttccgg cgacctggat gctggtggga taacgttcat
    22921 cccatcgttg gatagcaaag gagatcacga tgggtaaaac ccagcccttg ccaatattaa
    22981 ttactggcgg aggtcgccgc atcggcctcg ccctcgcatg gcatttcatt aatcaaaagc
    23041 aaccggtgat tgtcagctat cggacgcact atcccgccat tgatggactg attaaagcag
    23101 gtgcgcagtg tattcaggct gatttttcga ccaacgacgg tgtgatggcg tttgccgatg
    23161 aagtactaaa aagcacccat ggtctgcgtg ctattttgca taacgccagc gcgtggatgg
    23221 cggaaaaacc gggtgcgcca ctgaccgacg tactggcttg catgatgcag atccacgtta
    23281 ataccccata cctgctcaac catgctctgg aaagattact gcgtgggcac ggacacgccg
    23341 ccagcgatat cattcacttt accgattatg tggtggagcg cggtagcgac aaacatattg
    23401 cctatgctgc aagcaaagcg gcactggata atatgacccg ctcgtttgcc cgcaagctgg
    23461 caccggaagt gaaagtgaat tctattgcgc catcgctgat cctgtttaat gaacatgatg
    23521 atgccgaata tcgacaacag gcgctgaata aatcactgat gaaaaccgcg cctggcgaga
    23581 aagaagtgat cgacctggtc gattacttac ttaccagctg ctttgtcacc ggacgcagtt
    23641 tccctcttga tggcggtcgt catctgcgtt aatgcagctt tatccagcat ataatcaaca
    23701 cccatgcgct tatcccccaa cacgcaacag aaccaacaaa agcggcgggc aagcgcatca
    23761 ttccggtgaa ataccacagc gacgccaggt aaacaaaata gggaataatc gaccacatac
    23821 taaaaatgat ggttgcgcgt aaggcttcaa tgccgcgttc gctggcaaca atataatgcg
    23881 cgataagcgc aaaggtcggg aaaagtggaa tcagcccggc gatataataa tttttcgttt
    23941 ttgctaaaac accaatcaac agcactacca gcgcaccgag cgcggctttg attaccagcc
    24001 ctatcttttt accttaacac ttccataaca agtcatcagt agaatacctg atgaaacctt
    24061 gtttagaaac gattgatagt aagtaaaaac agtgcggtgt attgtgacgt ttttatatct
    24121 accgtgaatg ttatgaacac tatcgtattt gtggaagatg atgcggaagt cggtgcactg
    24181 attgccgcat acctggcaaa acatgatatg caggttaccg tagagccgcg cggcgaccgg
    24241 gccgaagaaa ctattctgcg agaaaatccg gatttggtgt tactcgacat catgctacca
    24301 ggcaaggacg gcatgaccat ttgtcgtgat ttacgcgcaa agtggtccgg accgattgtt
    24361 cttctaacct ctctcgatag cgatatgaac cacatcctgg cactggaaat gggcgcctgc
    24421 gactatattc tcaaaacgac gccccctgct gttttgctag cgcgtttacg tttgcatttg
    24481 cgtcagaatg agcaagccac agtgaccaaa ggtattcagg aaacgtctct gactccctac
    24541 aaagccctgc atttcggcac gttgaccatc gatcccatca accgcgtagt caccctggct
    24601 aacactgaaa tctcgctctc gacagctgat ttcgaattat tgtgggaatt agctacccat
    24661 gccgggcaaa tcatggaccg cgatgcattg ctgaaaaatt tacgcggcgt cagttatgac
    24721 ggtctggatc gtagcgtgga cgtggctatt tcgcggttaa gaaaaaaact gctcgataac
    24781 gccgcagaac cttatcgtat taaaaccgtg cgtaacaaag gctatctttt tgcgcctcat
    24841 gcgtgggaat aagcgatgaa aaaactgttt atccagtttt acctgttatt gtttgtctgc
    24901 ttccttgtga tgtctctgct ggtcgggctg gtgtacaaat ttaccgccga acgcgcaggc
    24961 aaacagtcgc tggatgattt gatgaacagt tcgctgtatc tgatgcgcag cgaattgcgt
    25021 gagatccccc cacacgactg gggtaagacg ctgaaagaga tggatttaaa tctctctttc
    25081 gatctgcgtg tcgagccgct gagtaaatac catcttgatg atatttccat gcaccggctg
    25141 cgtggcggcg aaattgtcgc cctggacgat cagtacacgt ttttgcaacg tatcccgcgc
    25201 agccactacg tgctggcagt tggtcctgtt ccttatcttt attacctcca tcagatgcga
    25261 ttactggata tcgccctgat cgcttttatt gctatttccc tcgcctttcc ggtgtttatc
    25321 tggatgcgtc cgcactggca ggatatgtta aaactggaag cagcggcgca acgatttggc
    25381 gatgggcatc tcaatgaacg tatccacttt gatgagggtt cgagctttga acgacttggt
    25441 gttgcattta accagatggc ggacaatatc aacgccttga ttgccagcaa aaaacagctt
    25501 attgacggta tcgctcacga actgcgaaca ccgttagtgc gcctgcgtta tcgactggag
    25561 atgagcgata acctgagcgc cgccgaatcc caggcgttga accgcgatat cagtcaactt
    25621 gaagctttaa ttgaagagct tctgacttat gcccgactcg atcgcccaca aaacgagctt
    25681 catcttagcg aaccagacct gccgttgtgg ctgtcaacgc atctggcaga cattcaggca
    25741 gtaacgcccg ataaaacggt acggataaaa acgctcgcgc aaggccatta tgcggcgttg
    25801 gatatgcgct taatggagcg cgtgctggat aatttgctca ataacgccct gcgctactgt
    25861 cactcaacgg ttgaaaccag cctgctactg tcggggaata gagcgacatt aattgttgag
    25921 gatgatggcc cagggattgc cccagaaaac cgcgaacata tctttgaacc ttttgttcgc
    25981 ctcgatccca gccgggatcg ctcaaccggc ggctgcgggc tggggctggc cattgtccac
    26041 tctatagcac tggcaatggg cggtacggtt aattgtgaca ccagcgaact gggtggtgcc
    26101 cgcttctcgt tcagctggcc gttatggcat aacatcccgc aatttacctc tgcctgacac
    26161 tatgcgcacg gtggtcaagt catcaccatt gtgctataaa ataaggatgt tgtaactaaa
    26221 gtggttaata ttatggcgcg ttacgatctc gtagaccgac tcaacactac ttttcgccag
    26281 atggaacaag agctggctgc atttgccgct catcttgagc aacacaagct attggttgcc
    26341 cgcgtgttct ctttgccgga ggtaaaaaaa gaggatgagc ataatccgct taatcgtatt
    26401 gaggtaaaac aacatctcgg caacgacgcg caatcgctgg cgttgcgtca ttttcgccat
    26461 ttatttattc aacaacagtc cgaaaatcgc agcagcaagg ccgctgtccg tctgcctggc
    26521 gtgttgtgtt accaggtcga taacttttcg caagcagcgt tagtcagtca tattcagcac
    26581 atcaataaac tcaagaccac gttcgagcat atcgtcacgg ttgaatcaga actcccctcc
    26641 gcggcacgtt ttgaatgggt gcatcgtcat ttgccggggc tgattacact taatgcttac
    26701 cgcacgctca ccgttctgca cgaccccgcc actttacgtt ttggctgggc taataaacat
    26761 atcattaaaa atttgcatcg tgatgaagtc ctggcacagc tggaaaaaag cctgaaatca
    26821 ccacgcagtg tcgcaccgtg gacgcgcgag gagtggcaaa gaaaactgga gcgagaatat
    26881 caggatatcg ctgccctgcc acagaacgcg aagttaaaaa tcaaacgtcc ggtgaaggtg
    26941 cagccgattg cccgcgtctg gtacaaagga gatcaaaaac aagtacaaca cgcctgccct
    27001 acgccgctga ttgcactgat taatagggat aatggcgcgg gcgtgccgga cgttggtgag
    27061 ttgttaaatt acgatgccga caatgtgcag caccgttata aaccacaggc gcagccgctt
    27121 cgtttaatca ttccacggct gcacctgtat gttgcagatt aacccccggc tttcatactg
    27181 ccgaccatct gttctggccg tacccagcgg tcaaactcgg cttcgctaag ataccccagc
    27241 gcaagggccg ccgcttttaa ggtcagccct tctttatgcg cttttttggc gatctcggcg
    27301 gctttgtcat aaccaatgtg ggtgttaagc gcagtcacca gcatcagcga ttcattgagt
    27361 aattgattga ttcgctcacg attcggttca atgcccactg cgcagtgttt gttgaaactt
    27421 tccatgccat ctgccagcaa gcgcaccgat tgcaggaaat tatggatcac catcggacgg
    27481 aagacgttca gttcaaagtt accggaagcg ccacccatgt tgatcgccac atcgttcccc
    27541 atcacctgac agcagagcat ggttaatgct tcacactgcg tcgggttcac tttccccggc
    27601 atgattgagc tacccggctc attttccggg attgagattt caccaattcc gcagcgcggg
    27661 ccagaggcca gccagcggac atcattggca attttcatca gtgacgcagc caaccctttc
    27721 aatgcgccgt gcgcctgaac cagggcatca caggtcgcca gcgcttcaaa tttgttcggc
    27781 gcggtaacaa acggtgcaca ggtaatgact gccagttcat ctgctacgcg acgcgcatat
    27841 tccggatggg tatttagtcc agtacccacc gctgtaccgc ccagagccag ttccgctacg
    27901 tgaggcaggc tgtattcgat atgtttgaga ttatgctcca gcatcgctac ccagccggaa
    27961 atctcctgcc ccagcgttaa cggcgtcgcg tcctgcaagt gggtacgacc gattttgacg
    28021 atatcggcaa atgcgcggga tttttcactc agcgtctggg tcagggtttt aagttgtgga
    28081 atgagttgct tgcgcagcgc cagtagtgcc gcaacgtgca tcgccgtcgg aaagacatcg
    28141 ttggaacttt ggcttttgtt cacgtcgtcg ttagggtgaa ctttacgttc catcccgcgc
    28201 acgccgccga gtaattcact ggcccggtta gccaacactt cgttcatgtt catgttactt
    28261 tgcgtgccgg agccggtctg ccagatagcc agcgggaatt cgtcgtcatg ctgtcctgcc
    28321 agtacttcat ccgccgcctg ccgaatggcg ctcgctttct cttcagacaa caagcctaaa
    28381 tcttcattaa cttttgccgc tgcgcgcttg gttagcgcca gcgcatgaat cagtgaggtg
    28441 ggcattttct ccgtcgaaat gcggaaatgc tccagcgagc gctgagtttg tgcgccccac
    28501 agcttatctg ccgggacatc aatcgctccc atcgaatctt tttcgctgcg tactgtattc
    28561 atggcctgct cctcacctga ttaatttttt ctttctgttt tgctttcgtt aagcaacttt
    28621 tagctcactc attatttacc atttgataac aaacgtttgg tctttcgtgc catgtaaaaa
    28681 aaccgccccg aagggcggct ctgtttattt cacacagcgg gtgcattgtg tgagttgtat
    28741 ctgctggaag aagtcatttc ctttatcatc cacaaggata aacgccggga aatcttccac
    28801 ttcaattttc cagatggctt ccattcccag ttccggatat tcaacacatt ccaggctctt
    28861 aatgcttccc tgcgccaata cagctgccgg accaccgata ctgccgaggt agaagccgcc
    28921 gtgtttttta caggcatccg tcacctgctg gctgcggttg cctttcgcca gcatgatcat
    28981 acttccgccc tgcgcttgca gttgatcgac ataagaatcc atccgtccgg cggtcgttgg
    29041 gccaagagaa ccggaggcat aaccttccgg cgttttggcc ggacccgcgt agtaaatcgg
    29101 atgatcttta atgtactgcg gcagcccttc accgttatcc atccgctctt tcagtttggc
    29161 gtgagcaata tcacgaccga cgataatcgt gccgttaagc gataagcgtg tagaaacggg
    29221 atactgcgac aactgtgcga ggatctcttt catcggacgg ttaaggtcaa cgcgcacggc
    29281 ttcgccttct cctgctttgc gcagctcttc cgggatatat ttgcctggat tatgttccag
    29341 tttttcgatc caaatcccct gacggttgat cttcgctttg atattacggt cagcagaaca
    29401 ggagacgccc ataccgaccg ggcaggatgc gccgtgacgt ggcaggcgaa tcacgcggat
    29461 gtcatgagcg aagtatttac caccaaactg cgcacccaga ccaagatttt gcgcttcgat
    29521 cagcaattct ttttccagtt ccacatcgcg gaacgcctga ccgtgctcat tcccttccgt
    29581 tggcagttca tcatagtatt tagcggaagc gagtttcacc gttttaagat tcgtttctgc
    29641 agaagttcca ccaataacga acgcaatatg atatggagga caggccgccg tacccagcgt
    29701 gcgcatcttc tcaaccaggt aatttttcag ttttcccggc gtcagtaacg ctttggtttc
    29761 ctgatagaga tacgtcttgt ttgccgaacc gccacctttg gcgatacaga ggaatttgta
    29821 ctcgtcgcca tcaacggcat aaagatcgat ctgcgctggc aagttggtgc cggtattcac
    29881 ctctttatac atatccagcg gcgcgttttg cgagtagcgc agattatcct cgatataagt
    29941 gttataaaca ccgcgcgcca gcgctgcttc atcaccgcca ccggtccata cacgctgccc
    30001 ttttttacca acaataatcg cggtgccggt atcctgacag gttggcagaa cgccttttgc
    30061 cgcgatgtcg gagttacgca ggaattgcag cgccacatat ttatcatttt cgctggcctc
    30121 cgggtcacgc agaatgtcgg ccacctgttg ttggtgagcc ggacgcagca taaatgacgc
    30181 atcatgaaac gcctgacgcg ccaacagagt taacgcttcg ggtgcgactt tcaaaatctc
    30241 ctgcccttca aattcagata cgctaacgtg ttcgctggtt agcaggtaat actcagtatc
    30301 atcttttttg agtggaaaag gagcctgata atgaaagggt ttgtttgaca ttgttctctc
    30361 acttactgcc tggtttggtt atgctctggg cgggtgttcc gttgccctgt taaaagcgag
    30421 taacaatatc ctacacactt ttttaacaaa aactgagact agtacgactt tttgcggctc
    30481 caggttactt cccgtaggat tcttgcttta atagcgggat taatttccac attaaaacag
    30541 ggattgatca tgcaaaaact cattaactca gtgcaaaact atgcctgggg cagcaaaacg
    30601 gcgttgactg aactttatgg tatggaaaat ccgtccagcc agccgatggc cgagttgtgg
    30661 atgggcgcgc atccaaagag cagttcacgt gtgcacgatg ccgcaggaga tatcgtttca
    30721 ctgcgtgatg tgattgagag tgataaagcg actcttttgg gagatgccgt tgccaaacgc
    30781 tttggcgagt tgcctttcct gttcaaagta ttatgcgcag cccagccgct ctccattcag
    30841 gttcatccaa acaaacgcaa ttctgaaatc ggttttgcca aagaaaatgc tgcaggtatt
    30901 ccgatggatg ccgctgagcg taactataaa gatcctaatc acaagcctga gctggttttc
    30961 gccctgaccc ctttcctcgc gatgaacgcg tttcgtgagt tttccgagat tgtctccctg
    31021 ctccagccag tcgcaggtgc acatccggcg attgctcact ttttacaaca gcctgatgcc
    31081 gaacgtttaa gcgaactgtt cgccagcctg ttgaatatgc agggtgaaga aaaatcccgc
    31141 gcgctggcga ttttaaaatc ggccctcgac agccagcagg gtgaaccatg gcaaacgatt
    31201 cgtttaattt ctgaatttta cccggaagac agcggtctgt tctccccgct gttgctgaat
    31261 gtggtgaaat tgaatcctgg cgaagcgatg ttcctgttcg ctgaaacacc gcacgcttac
    31321 ctgcaaggcg tggcgctgga agtgatggcg aactccgata acgtgctgcg tgcaggtctg
    31381 acgcctaaat atattgatat tccggaactg gttgccaacg tgaagttcga agccaaaccg
    31441 gctaaccagt tgttgaccca gccggtgaaa caaggtgcag aactggactt cccgattcct
    31501 gtggatgatt ttgccttctc gttgcatgac cttagtgata aagaaaccac catcagccag
    31561 cagagtgccg ccattttgtt ctgcgtcgaa ggcgatgcaa cgctgtgtaa aggttctcag
    31621 cagttacagc ttaaaccggg tgaatcagcg tttattgccg ccaacgaatc accggtgact
    31681 gtcagaggcc acggccgttt agcgcgtgtt tacaacaagc tgtaagagct tactgaaaaa
    31741 attaacatct cttgctaagc ttattaaagg cttataacac cttcaggcgg ccagtccgcc
    31801 tgatttcatt ttatggataa tcattatgaa taaatcgctg gtagcggtag gcgtcattgt
    31861 tgcgctaggc gtagtctgga caggcggcgc atggtataca ggcaagaaga ttgaaaccca
    31921 tctcgaagac atggtcgcgc aggcgaacgc gcaactcaaa ctgacagctc ctgaatccaa
    31981 cctggaagtc agttatcaaa actatcatcg cggcgtattc agcagccagt tgcaactgtt
    32041 ggtgaaaccc attgccggaa aagtaaatcc gtggattaaa agcggtcaga gcgtcatctt
    32101 caacgaatcg gttgatcatg gtcccttccc gcttgcccag cttaaaaaac tgaacctgat
    32161 cccgtcgatg gcatcaattc aaaccacgct ggttaataac gaagtaagca aaccactgtt
    32221 tgatatggca aaaggtgaaa cgccttttga gattaactcc cgcattggtt acagcggaga
    32281 ttccagttcc gacatttcgc tcaagccact gaattacgag caaaaggatg aaaaagtcgc
    32341 atttagcggc ggcgagttcc agttaaatgc agacagagac ggcaaagcta tctccctttc
    32401 cggggaggcg caaagtggtc ggatagacgc ggttaacgaa tacaaccaga aagtgcagtt
    32461 aacctttaat aatctgaaaa ccgacggttc cagcacgctg gcaagttttg gtgagcgcgt
    32521 aggaaaccaa aaactgtcgc tggaaaaaat gaccatttca gtggagggca aagaactggc
    32581 actgctggaa ggcatggaga tcagcggtaa atcggatctg gtcaatgacg gtaaaacggt
    32641 caatagccaa ctggattact cgctaaacag cctgaaggta cagaatcagg atctgggcag
    32701 tggcaagctg actttaaaag tcggccaaat tgatggcgaa gcctggcatc agtttagcca
    32761 gcaatataac gcgcaaactc aggcgctgct ggcacagcca gaaattgcca acaatcccga
    32821 actttatcag gagaaagtga cggaagcctt ctttagcgct ctgccgctga tgctgaaagg
    32881 cgatccggtg attactatcg cgccgctaag ctggaaaaac agtcagggtg aaagtgcgct
    32941 gaatctgtcg ctgttcctga aagatccggc aacgactaaa gaagcgccgc aaacgctggc
    33001 gcaggaagtg gaccggtcgg ttaaatctct ggatgcgaaa ctgaccattc cggtggatat
    33061 ggcaactgag ttaatgactc aggtagcgaa gctggaaggt tatcaggaag atcaagcgaa
    33121 aaaactggcg aaacagcaag ttgaaggtgc atcagcaatg gggcagatgt tccgtctgac
    33181 caccttgcag gacaatacca tcaccaccag cctgcaatat gctaacggtc agataacgtt
    33241 aaacgggcag aaaatgccac tggaagattt cgtgggtatg tttgcgatgc cggcattaaa
    33301 cgttccggct gtacccgcta ttccgcagca gtaattcaca gccaaaacgc cggagatttt
    33361 tctctccggc gttttttctt acttcagcat aaagtcatac tgaatactca ttttcacatc
    33421 ccagtaattc aggcgatcgc ccagcgcact gccatcgcgc ttttcccgcc ctttatcggc
    33481 agcaagaaag aaattcatcc cttgtaacgg cccttcgctg acccgatagt tcataccacc
    33541 taagataccc gcgccttcaa tccgcacatc tcgtttactg gattcatagc cgcgagtcac
    33601 aatcggtgca aatgtcagcg taaattgcgg agttaaacga tagttaacgc ccaattgcca
    33661 ggactgcatt ccttcacggt tacgatcaag acttaatgaa aagggatagc caatgtcggt
    33721 gtccaccacg gcaccattca ttgcatggcg caaataacca ccactgccaa atacgcttcc
    33781 ccacggcgca atccaggtca attgtccact aaccaacgcc gtttcattgg gctggctggt
    33841 attacgactt aaaccttcga gttgcgcgta aaagccgagc aactcccctt tcaacgtata
    33901 attcgggtcg agcggtatgc ttttctcgag aatactttgt atctgattgg aaacatcttt
    33961 ctgtaccgat gcccccagag aatagcgact ggtatcatca tgccaactgg cggctaaaaa
    34021 agaacgtgcg cgatgtttat cgtattttcc cgtctggctg ttatacaccc cttcatcacg
    34081 aataccggtt gttgaatcgg tccataaaaa tactcgtgaa cgccacattc cccacaggtc
    34141 aatatttggc gttaaagccc aataacctaa tgctccttca gaaactgtcg gtaatgtacg
    34201 ttgcgaactg ccagccatta acggaatacg ataaggtaag ct
//
LOCUS       DAZFFE010000040        33662 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFFE010000040 DAZFFE010000000
VERSION     DAZFFE010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.040"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(229..699)
                     /gene="ylaC"
                     /locus_tag="ACHJ69_002136"
     CDS             complement(229..699)
                     /gene="ylaC"
                     /locus_tag="ACHJ69_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO5015124.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(815..1366)
                     /gene="maa"
                     /locus_tag="ACHJ69_002137"
     CDS             complement(815..1366)
                     /gene="maa"
                     /locus_tag="ACHJ69_002137"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO5015125.1"
                     /translation="MSTEKEKMIAGELYHSADEALSRDRLHARRLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFCCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(1540..1758)
                     /gene="hha"
                     /locus_tag="ACHJ69_002138"
     CDS             complement(1540..1758)
                     /gene="hha"
                     /locus_tag="ACHJ69_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO5015126.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(1784..2158)
                     /gene="tomB"
                     /locus_tag="ACHJ69_002139"
     CDS             complement(1784..2158)
                     /gene="tomB"
                     /locus_tag="ACHJ69_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO5015127.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(2704..5853)
                     /gene="acrB"
                     /locus_tag="ACHJ69_002140"
     CDS             complement(2704..5853)
                     /gene="acrB"
                     /locus_tag="ACHJ69_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO5015128.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTNYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(5876..7069)
                     /gene="acrA"
                     /locus_tag="ACHJ69_002141"
     CDS             complement(5876..7069)
                     /gene="acrA"
                     /locus_tag="ACHJ69_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO5015129.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            7211..7858
                     /gene="acrR"
                     /locus_tag="ACHJ69_002142"
     CDS             7211..7858
                     /gene="acrR"
                     /locus_tag="ACHJ69_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO5015130.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLRKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            7986..11348
                     /gene="mscK"
                     /locus_tag="ACHJ69_002143"
     CDS             7986..11348
                     /gene="mscK"
                     /locus_tag="ACHJ69_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO5015131.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQVQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VSKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQTLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNATEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            11409..11507
                     /locus_tag="ACHJ69_002144"
                     /pseudo
     CDS             11409..11507
                     /locus_tag="ACHJ69_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11560..11721)
                     /gene="rsmS"
                     /locus_tag="ACHJ69_002145"
     CDS             complement(11560..11721)
                     /gene="rsmS"
                     /locus_tag="ACHJ69_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO5015132.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(11735..12262)
                     /gene="priC"
                     /locus_tag="ACHJ69_002146"
     CDS             complement(11735..12262)
                     /gene="priC"
                     /locus_tag="ACHJ69_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO5015133.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLATQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARATDLVEQQTLHREVEAYEARLARCR
                     HALDKIENRLARLTR"
     gene            12332..12709
                     /gene="ybaN"
                     /locus_tag="ACHJ69_002147"
     CDS             12332..12709
                     /gene="ybaN"
                     /locus_tag="ACHJ69_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO5015134.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGAKPRAILLILITFTISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            12862..13413
                     /gene="apt"
                     /locus_tag="ACHJ69_002148"
     CDS             12862..13413
                     /gene="apt"
                     /locus_tag="ACHJ69_002148"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO5015135.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            13542..15473
                     /gene="dnaX"
                     /locus_tag="ACHJ69_002149"
     CDS             13542..15473
                     /gene="dnaX"
                     /locus_tag="ACHJ69_002149"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO5015136.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIKLRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAQTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLLRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    14806..14870
                     /gene="dnaX"
                     /locus_tag="ACHJ69_002149"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            15526..15855
                     /gene="ybaB"
                     /locus_tag="ACHJ69_002150"
     CDS             15526..15855
                     /gene="ybaB"
                     /locus_tag="ACHJ69_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO5015137.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            15855..16460
                     /gene="recR"
                     /locus_tag="ACHJ69_002151"
     CDS             15855..16460
                     /gene="recR"
                     /locus_tag="ACHJ69_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO5015138.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRKENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            16570..18444
                     /gene="htpG"
                     /locus_tag="ACHJ69_002152"
     CDS             16570..18444
                     /gene="htpG"
                     /locus_tag="ACHJ69_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO5015139.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTCDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTETRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            18625..19269
                     /gene="adk"
                     /locus_tag="ACHJ69_002153"
     CDS             18625..19269
                     /gene="adk"
                     /locus_tag="ACHJ69_002153"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO5015140.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRAALEKILG"
     gene            19401..20363
                     /gene="hemH"
                     /locus_tag="ACHJ69_002154"
     CDS             19401..20363
                     /gene="hemH"
                     /locus_tag="ACHJ69_002154"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO5015141.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQYSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALEMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(20338..21306)
                     /gene="aes"
                     /locus_tag="ACHJ69_002155"
     CDS             complement(20338..21306)
                     /gene="aes"
                     /locus_tag="ACHJ69_002155"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO5015142.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNVGAPEMATRAYRVPTKYGQVKTRIFYPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYSGMLHAFLHYSRMMKTADDALRDGARFFSDRLQQD"
     gene            21458..22762
                     /gene="gsk"
                     /locus_tag="ACHJ69_002156"
     CDS             21458..22762
                     /gene="gsk"
                     /locus_tag="ACHJ69_002156"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO5015143.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRIDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(22818..24494)
                     /gene="ybaL"
                     /locus_tag="ACHJ69_002157"
     CDS             complement(22818..24494)
                     /gene="ybaL"
                     /locus_tag="ACHJ69_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO5015144.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(24732..25952)
                     /gene="fsr"
                     /locus_tag="ACHJ69_002158"
     CDS             complement(24732..25952)
                     /gene="fsr"
                     /locus_tag="ACHJ69_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO5015145.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            26170..27521
                     /locus_tag="ACHJ69_002159"
                     /pseudo
     CDS             26170..27521
                     /locus_tag="ACHJ69_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
     gene            complement(27558..28037)
                     /gene="ybaK"
                     /locus_tag="ACHJ69_002160"
     CDS             complement(27558..28037)
                     /gene="ybaK"
                     /locus_tag="ACHJ69_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO5015146.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAASDLAKILDAKFADIARRD"
     gene            complement(28241..29035)
                     /gene="ybaP"
                     /locus_tag="ACHJ69_002161"
     CDS             complement(28241..29035)
                     /gene="ybaP"
                     /locus_tag="ACHJ69_002161"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO5015147.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACETLEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDNGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            29168..29469
                     /locus_tag="ACHJ69_002162"
                     /pseudo
     CDS             29168..29469
                     /locus_tag="ACHJ69_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057540.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            29505..29846
                     /gene="ybaQ"
                     /locus_tag="ACHJ69_002163"
     CDS             29505..29846
                     /gene="ybaQ"
                     /locus_tag="ACHJ69_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO5015148.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(29904..32408)
                     /gene="copA"
                     /locus_tag="ACHJ69_002164"
     CDS             complement(29904..32408)
                     /gene="copA"
                     /locus_tag="ACHJ69_002164"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO5015149.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALVVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADIDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVNTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            32670..33602
                     /gene="glsA"
                     /locus_tag="ACHJ69_002165"
     CDS             32670..33602
                     /gene="glsA"
                     /locus_tag="ACHJ69_002165"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO5015150.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKVGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENTEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTIELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGSSVRGQKMVASVAKQLGYNVFKG"
BASE COUNT         8430 a         8724 c         8685 g         7823 t
ORIGIN      
        1 acaggcagca atactgaaag aatcagtact cccgaaataa ggccgaccag atgtcgtgtt
       61 ctcaccagca tatccttatt aacacagtgt agggaaagta tatctgattg tggaaaaagg
      121 ctggcaggag ataaggaggt agagctggta tttcatgaag tgatgggtgg aaacgatcgt
      181 tgtcatttga ccaggcgtag agctttacgc ctggtatgta cccctaactt aagatgttga
      241 ttcggcgcga gcgagggtaa aaatatcgta aaaagatagt tcacctttac ggacgatcat
      301 tttttgcagt tgttctttat gaacatccgc gacacgcgga gagttcagga tggcttcaac
      361 tagcgcggca ggtacaaagc gcgtgttgta ccattcgccg ttatagcaaa cgcggaaatc
      421 cagcacgtcg atatcttcat agcggtagcg tgggtagaca tcgaaaaaga agaaaccatt
      481 aagcaggata aataatacaa ccagtagcca gacagagcct gaaagcgttt cggactgcaa
      541 catcaccgcc agggtggcaa aaaaagcaac gtacataccg ataaacagcc ccggatgttt
      601 acggataaaa ctgatactaa agcggggttt gttgtcgcgt ttttcgcggg tattcagaga
      661 ctcaatcgtt tcggtcagca ggcgttgtat ttcggtcatc gtttgccttc gtgatatctg
      721 caactttcca gggaacaccc tattttaacg tgggaatagt agaggggaag taacagattt
      781 gccacaattt tgcataacag ttgagaaaag ctggttacaa ttttttaatg attctggctg
      841 gattaccgcc cacgacaacg ttgtccggga catcttttgt gacaactgca cctgaggcta
      901 ccacgacgtt atcaccaatg gtcacaccag ggttaatgac tgcacgtccg ccaatccaga
      961 cgttattacc gatggtgacg ggtttcccca gttcagcacc gctattacgt gctacagggt
     1021 cgatgggatg tgttgccgtg tagatatgaa cgcctggtgc caacatacag ttatcaccaa
     1081 tgcgaatagg gcagacatca agcatcacgc aatcgaagtt ggcgaaaaaa ttattgccga
     1141 gaaaaatgtt atagccatag tcacagcaaa acgttggctc aatataagcc tctgtcacct
     1201 gaccgaataa atcagcaaga atttgctggc gtaatgtgtg ctcttccgcc agggaatgat
     1261 tgtatcggtg aataagccga cgagcgtgca ggcgatcgcg agataacgcc tcatctgccg
     1321 agtgatacaa ctcaccagca atcatctttt ctttttctgt gctcattact gacttccggt
     1381 tgctgaaaat gcggcaacac agtaagacga aaagtcagtg aagagaacgt tcccattaca
     1441 tctgtgtgat gaagatcaca tagggtatgt cttcatggcg aaaaaaagta taaaattctt
     1501 aataaacagc cggttatagc tccgaaagcg agttatttat tagcgaataa atttccatac
     1561 tgaggaaggg atcttgtcgt acagtttatt catggtcaat tcggcgaggc ggtgatctgc
     1621 agctgagtaa aataccgcca gttcattatc tgataattcg tatttatttt tctcgataac
     1681 acgctccagc gtgtcaattg tctgacaacg acgtaaacgc attaaataat cggttttcgt
     1741 taaaggtttt tcggacatac ttctacccat ggttgtaata attctaacaa gataaactcg
     1801 caggattctc tttcgtcgca ttgacaaaac aacggaatag tcgattacct gacttccgcc
     1861 atttctgaag atcctgcata ttaataccat aactactgaa caacataaag gtgtcatcca
     1921 gatattcgtc gatctgctca atgagcttat tgtcttcatt atacttaatt ttgtaattaa
     1981 gtgcgaaggt cgcaatatgc tcaatcagtt cattcaactg gaggttgatc gccgaggttg
     2041 ggtcgttaac ccagccatga ttgctttctt caaggtttgc aaggcagtca tgatacaggg
     2101 tttcacagag aaacttaagc tgtgcgatat catgtctttt gggtgagtat tcatccataa
     2161 cgcgtcccct tcttagcggt tgaactaacg gacacctttc gggatggaaa aaacttactg
     2221 acctggactt gccctctcgt ttgttagctt aactataagc cactctttgt aggttttcat
     2281 cgcattatta cgaaaaatta caattaggaa ggaagcggga caggactcac tttttcatac
     2341 tagcaccaac ccgcagcagg taaaagcagt ttgctcgaac tgttcataac atttcagaat
     2401 tatcgccaga agaaattggt gacactaata ccaggattgc tctgaatatg acgtaataac
     2461 cgaggaatga ataaagaatt accgcaaata attaagaata gcctctaaat ggttatggat
     2521 tattaaatct attagcacta cttaatataa attaactaac aatcagtatt ttttatgaat
     2581 tttatgcggg gttaatactg gttttcgtat gagatcctga gttggtggtt caatacatcc
     2641 ttaatgttcg taggttatgc ataaaaaagg ccgcttgcgc ggccttagtg attacacgtt
     2701 gtatcaatga tgatcgacag tatggctgtg ctcgatatct tcattcttgc ggctaaagcg
     2761 gcggcgaacc accacaaaga ataccggaac gaagaagatt gccagtaccg ttgcggtcac
     2821 catcccgccc attacaccgg tacctactgc gttctgcgcg ccggaaccag caccagtact
     2881 gataaccagc ggcataacgc cgaggataaa cgccaacgag gtcatcagga ttgggcgtaa
     2941 acgcatccgc accgcatcaa gcgtcgcttc aatcagacct ttaccttctt tatccatcaa
     3001 gtctttggcg aattcgacga taagtatcgc gttcttcgcc gacaaaccaa tggttgtgag
     3061 caggcctacc tggaagtaaa cgtcattggt caggccacgg aaggtggcag ccagcaacgc
     3121 accgataacc cccagcggaa cgactagcat aacggagaac ggaatcgacc agctctcgta
     3181 cagcgcagcc agacacagga acacgacaat caacgaaatc gcgtacagtg aaggtgcctg
     3241 gttgccggag agacgttcct gataggacat tcccgtccag tcatagccaa caccggtagg
     3301 cagtttgctc gccagttgtt ccatcagctc cattgcttca ccggtacttt tacccggtgc
     3361 cgcctggcct aagatttcca tagatggcag gccgttgtaa cgttccagac gcggcgaacc
     3421 gtactcccaa cgagaagagg agaacgccga gaacggcacc atctgaccat cagcagcacg
     3481 aacataccag tcgccgatat catccggcag catacggtat ttcgcttctg acatgacgta
     3541 aactttcttc acacgaccgc ggtcgataaa gtcgttcaca tagctaccgc cccatgcagc
     3601 gcccagagtg gtgttaatgt cgttgataga aacacccagc gcctgcgctt tttcctggtc
     3661 gatatcaatc ttaaactgcg gggtatcttc cagaccgttt ggacgtacgc tggtcaacat
     3721 atcagggtgc ttcgctgctt ctgcaagcaa ctggttacgc gcctgagtca gtttttcgtg
     3781 accaaggcca gcctggtcaa tcagctcaaa gtcaaagccg gttgcggtac ccagttccac
     3841 gattgcgggc aggttaaagg cgaaaaccat cgcatctttg atttgcgaga aagcgcgtgt
     3901 tgcacgcatg gtaatcgctt caactttgtt ttcttcgccc ggacgatcgg cccaatcctt
     3961 caaggaaacg aacgcaatac cggtattctg accacgtccc gcaaagccga agccgttaac
     4021 ggcgaacacc gactcaacgt tgttcttttc tttggtcaga tagtaattcg ttacctcatt
     4081 gagcactttc tgcgtacgtt cctgcgttgc acctgctggc agctgaacca tggtcataaa
     4141 tacgccctgg tcctcatctg gcaagaagga gcttggcaga cgcacgaaca gataggccat
     4201 accgaccacg atgatcagat acagcaccag gtaacgcccc gtactgcgca gaataccgcc
     4261 tacgctgtcg gtgtagtggt gcgtgctctt ctcgaacatg cggttaaacc agccgaagaa
     4321 gcctttttta ccttccccgt gatcgccttt ggcaatcggt ttcagcatgg tggcacaaag
     4381 agccggagtc aggatcaacg ccaccagtac cgacagcgcc attgctgaaa caatggtaat
     4441 agagaactga cgatagatag caccagtaga accgccaaag aaggccatcg gtacgaatac
     4501 cgccgacagt accatcgcga taccgaccag agcgccctga atctgcccca tcgacttacg
     4561 ggtggcttct tttggcggca aaccttcttc cgccatcaca cgctcaacgt tttctaccac
     4621 aacgatagcg tcatccacca acaggccgat ggcgagcacc atcccgaaca ttgttagcgt
     4681 gtttatcgag aagccaaagg cggcaaggac ggcaaaggtc ccgagcaata ccaccggtac
     4741 ggcaatggtc ggaatcaacg tcgcgcggaa gttctgcaag aacagataca ttaccaggaa
     4801 cacgaggatg atcgcttcga ccagcgtttt aaccacttcg tgaatagaga ttttcacgaa
     4861 cggtgtggtg tcatacgggt aaacaatttt cagacccgac gggaagaacg gttccatctt
     4921 cgccagttca gcacggattg ccgcagcggt atccagcgcg ttcgcaccgg tcgccagctt
     4981 gatccccaga ccggaagccg gttggccgtt aaactctgcg atgatgtcgt agttctcacc
     5041 acccagctca attttcgcca catcacgcag cagcacgcgg gaaccatcct gattcacttt
     5101 cagcaggatt ttgccgaact cttcagtaga ggtcagacgc gtctgagcaa taatagaggc
     5161 gttaagctgt tggcctttca ccggcggcgt accaccgagc tgaccagctg caacctgggc
     5221 gttctgtgct ttgatggcgg taatgacatc aaccggcgtt agctggaatt tgttcagttc
     5281 attcgggttc atccagatac gcatcgcgta ctgtgaaccg aacaactgaa cgtcacccac
     5341 gcctgacgta cggctgatgg catctttcat attcgccgcc acgtagtcgg agatatcctc
     5401 ctgcgtcatg gtgccatcgg tgttgataac gccgacaacc atcaggaagc tgctggatga
     5461 tttctcaacg ctcacccctt gctgctgtac ttcttgtggc agcaacggca tcgccagctg
     5521 cagtttgttc tgcacctgaa cctgcgcgat atccgcatca gtaccagact caaaggtcag
     5581 ggtgatctgc acggtacccg tggagtcact gttagaggac atgtacatca ggttatcgat
     5641 accgttcata ttctgttcga taacctgtgt caccgtgtcc tgcactgttt tcgcatcagc
     5701 gccagggtag gaggcggaga tcgttactgc cggcggtgca atcgtaggat attgcgccac
     5761 cggcagtttg aggatcgcca gcccccctgc caacatgatg ataatggcga tcacccacgc
     5821 aaaaatcggg cgatcgataa agaaattagg catgtcttaa cggctcctgt ttaagttaag
     5881 acttggactg ttcaggctga gcaccgcttg cggcttgctg gttattatca gcggtaactt
     5941 cttgtgcttt tacctggaca ccaggacgca ctttctgcag cccacttatt actacgcgat
     6001 cgcctgcttt cagaccttct gtcaccagcc acttatcgcc gatagcctgg cttgcaacga
     6061 tcggacgggt ttccactttg tcatccgcgc caaccaccag tacggtggca tcgccacgcg
     6121 gcgtacgggt tacgccctgt tgcgggacta aaatagcgtt tggattaagc ccttcttcca
     6181 gacgtgcacg cacgaacata cccggcagca gagtgtgatc cgggttcggg aagatagcgc
     6241 gtagggtgat agacccagtg gtctgatcaa cggtaacgtc agagaattcc agcgtaccgt
     6301 cctgcgggaa cttaatgccg tcactggtga tcagcgacac tttggctttg ccgttctctt
     6361 gtttcagcgt gccattcgcc agttcctgtt tcaggcgcag gaagtcgttg ctggactggg
     6421 tcacatcaac gtagatcgga tcaagttgct gcacggttgc cagcgcagtc gcctgaccgt
     6481 tctgtaccaa tgcgccttcc gtcacgtttg acttaccaat gcgaccacta atcggagagg
     6541 taactttggt gtaagccaga ttgattcgcg cagtttcaac ggcagctttc gccgcagtta
     6601 ccgcagcatt cgcctgttgc gcatcagcca gagcctgatc gtactcttgc ttactgatgt
     6661 actgagtacc gagcaatttc tgataacgat tcaccgtcaa ttgcgcgata ttggctgcag
     6721 cctgggcttt cgccagatca cctttcgcac tgtcgtatgc cgcctgatag gtcgcaggat
     6781 caatctgata gagagagaca cctgcttcga tgtcgctacc ttctttgaaa ttacgcttca
     6841 ggataatccc gctaacttga ggacgaactt ctgcgatccg gtaggcactg gtgcgacccg
     6901 gaagctcggt tgtgatctgc agaggttcag ttttgactgt tactacgcca acggcgggca
     6961 tctgctggcc accttgttgg gcctgtttgt cgtcacatcc tgttagggct aagctgcctg
     7021 agagcatcag aacgaccgcc agaggcgtaa accctctgtt tttgttcata tgtaaacctc
     7081 gagtgtccga tttcaaattg gtcaatggtc aaaagttaat aaacccattg ctgcgtttat
     7141 attatcgtcg tgctatggta catacattca caaatgtatg taaatctaac gcctgtaaat
     7201 tcacgaacat atggcacgaa aaaccaaaca agaagcgcaa gaaacgcgcc aacacatcct
     7261 cgatgtggct ctacgtcttt tctcacagca gggggtatca tccacctcgc tgggtgagat
     7321 tgcaaaagca gctggcgtta cgcgcggtgc aatctactgg cattttaaag acaagtcgga
     7381 tttgttcagt gagatctggg aactgtcaga atccaatatt ggtgaactcg agcttgagta
     7441 tcaggcaaaa ttccctggcg atccactctc agtattaaga gagatattaa ttcatgttct
     7501 tgagtccacg gtgacagaag aacggcgtcg attattgatg gagattatat tccacaaatg
     7561 cgaatttgtc ggagaaatgg ctgttgtgca acaggcacaa cgtaatctct gtctggaaag
     7621 ttatgaccgt atagaacaaa cgttaaaaca ttgtattgaa gcgaaaatgt tgcctgcgga
     7681 tttaatgacg cgtcgcgcag caattattat gcgcggctat atttccggcc tgatggaaaa
     7741 ctggctcttt gccccgcaat cttttgatct tagaaaagaa gcccgcgatt acgtcgccat
     7801 cttactggag atgtatctcc tgtgccccac gcttcgtaac cctgccacta acgaataacc
     7861 ctgaatctga ctccaggaat tttcctggac attttcgtcg ttgctattct ggttcactgc
     7921 gtcgtgatat tcttgcggtt tgactttttc aggtcgttct tcaggttcag aaaccttcat
     7981 tcatcatgac tatgttccag tattacaaac gatcacggca ttttgttttt tcagcattta
     8041 ttgcttttgt ttttgtcttg ttatgccaga acacggcgtt tgcacgggcg tcatcgaatg
     8101 gtgatttacc gacaaaagcg gacctgcagg cacaacttga ctcactgaat aaacaaaaag
     8161 atctttctgc tcaggacaaa ctggtgcagc aggatctgac agatacatta gccaccctcg
     8221 ataaaatcga tcgcgtaaaa gaagagacag ttcagctacg gcaaaaagtc gctgaagcgc
     8281 cggaaaaaat gcgccaggcg acagcggcgt taacagcact tagtgatgtc gataacgacg
     8341 aagaaacgcg caaaattctg agcacgcttt cgttgcgcca gctggaaact cgcgttgctc
     8401 aggcgctgga cgatttgcaa aacgcacaaa atgatctggc gtcttataac agccaactgg
     8461 tttcgctaca gacgcagccc gaacgcgtac aaaatgcgat gtataacgct tcgcagcagc
     8521 tgcaacaaat tcgcagtcgt ctggatggga ctgatgtcgg cgagactgcc ttacgtccca
     8581 gccagaaagt gttaatgcag gtccagcagg ctttgctgaa tgccgagatt gaccagcagc
     8641 gtaaaagcct ggaagggaac actgtcttgc aggatacctt gcaaaagcag cgtgattacg
     8701 tgacggcgaa cagcgcccgt ctggagcacc agttacaact gttgcaagaa gcggtaaaca
     8761 gcaagcgcct gactttaacc gaaaaaacgg cgcaggaagc tgtctccccg gatgaagccg
     8821 cgcgtattca ggctaatccg ctggtgaagc aggaactgga aattaaccag cagttaagtc
     8881 agcgtctgat taccgcgact gaaaacggta atcagttgat gcaacaaaac attaaagtca
     8941 aaaactggct ggagcgtgcg ctgcaatcgg aacgtaatat taaggagcag attgccgttc
     9001 tgaagggcag tctgctgttg tcacgtatcc tgtaccagca acaacaaaca ctcccctcgg
     9061 cagatgaact ggaaaacatg accaaccgca tcgcggattt gcgtctcgaa cagtttgaag
     9121 tcaaccagca gcgtgacgcg ctgttccaga gcgatgcgtt cgtcagcaaa ctggaagaag
     9181 gtcacaccaa tgaagttaac agcgaagttc acgatgcgtt attgcaagta gttgatatgc
     9241 gccgtgaatt gctggatcag cttaacaaac agttgggtaa tcagctgatg atggccatta
     9301 acctgcaaat taaccagcag cagttaatga gcgtatcgaa aaacctgaaa tccatcctga
     9361 cgcagcaaat tttttgggtg aacagtaacc gtccgatgga ctgggactgg attaaagcgt
     9421 tcccgcaaac gctgaaagat gaatttaagt cgatgaaaat cacggtgaac tgggaaaaag
     9481 cctggcccgc cgtattcatc gccttcctcg ctggtttgcc gctgctgttg attgctgggc
     9541 tgattcactg gcgtttaggc tggctgaaag catatcaaca aaaactggcc tcggcggtag
     9601 gttccctgcg taacgatagc cagctcaaca caccaaaagc gatccttatc gacctgatcc
     9661 gtgcgctgcc ggtgtgcctg attattctcg cggttggcct gattctgttg accatgcagc
     9721 tcaacatcag cgaactgcta tggtcgttca gcaaaaaact ggcgattttc tggctggtgt
     9781 ttggcctgtg ctggaaggta ctggagaaaa acggtgttgc cgtacgtcac tttggtatgc
     9841 cggaacagca gaccagccac tggcgtcggc aaattgtccg catcagcctc gcgttgctgc
     9901 cgatccattt ctggtctgtg gtggcagaac tctccccgct gcatctgatg gatgatgtgc
     9961 tggggcaggc gatgattttc ttcaacctgc tgctgattgc cttcctggtg tggccgatgt
    10021 gccgcgaaag ctggcgtgat aaagagtcgc acaccatgcg actggtcacc attaccgtgc
    10081 tgtcgattat cccgattgcg ttgatggtgc tgaccgccac gggttacttc tacaccacgc
    10141 tgcgcctctc cgggcgttgg attgaaaccg tttatttggt gatcatctgg aacctgctgt
    10201 accagacggt attgcgcggc ttaagcgtag cggcgcggcg tatcgcctgg cgtcgtgcac
    10261 tggcgcgtcg gcagaatctg gtgaaagagg gcgcagaagg tgccgaaccg ccggaagaac
    10321 ccaccattgc gctggagcag gttaaccagc agacgctgcg tattaccatg ttgctgatgt
    10381 ttgcgctgtt cggtgtcatg ttctgggcaa tttggtctga tttgatcacc gtgttcagct
    10441 atctcgacag cattacgctc tggcattaca acgctactga agctggtgct gcggtggtga
    10501 aaaacgtcac catgggcagt ctgttgtttg cgattatcgc ctcaatggtg gcctgggcgt
    10561 tgattcgcaa cctgcctggt ttgctggaag tgctggtgct ctcgcgactg aatatgcgcc
    10621 agggcgcgtc gtatgccatt actaccatcc ttaactacat cattattgcc gttggtgcga
    10681 tgacggtgtt cggatcgctg ggcgtctcgt gggataaact ccagtggctg gcggcagcat
    10741 tatccgtagg tcttggtttt ggtttacaag agatcttcgg taacttcgtt tccggtttga
    10801 tcattctgtt cgaacgtccg gtgcgcatag gcgacacggt aaccattggt agcttctcgg
    10861 gtacggtcag caagatccgt attcgtgcga caacgattac cgatttcgat cgcaaagaag
    10921 tgattatccc gaacaaagcg tttgttaccg agcgtctgat caactggtcg ttgactgaca
    10981 cgactacgcg tctggtgatc cgtctcggcg tagcctatgg ctccgatctg gaaaaagtgc
    11041 gtaaagtgtt gctgaaggcg gcgactgaac acccaagggt gatgcacgaa ccaatgccgg
    11101 aagtcttctt tacggcattt ggtgccagca cgttggatca tgagctgcgt ctgtatgtac
    11161 gtgaactgcg tgaccgtagc cgtactgtcg atgagctgaa ccgtactatc gatcagttgt
    11221 gccgtgaaaa cgacatcaac attgccttta accagcttga agtgcatctg cacaacgaga
    11281 agggcgatga ggtgacggaa gtcaaacgcg actacaaagg cgacgatccg acaccagcgg
    11341 ttgggtaata accaaagggg gcgacattta gttgccccga gattactaac aaagtgcgcg
    11401 ttgttcatgc cggatgcggc gtgaccgcct tatccggcct acgaaaccgc aagaattcaa
    11461 tatattgcag gagcggtgta ggcctgataa gcgtagcgca tcaggcagtt ttgcgtttgc
    11521 ccacaatctt aggggacatt tagcggcccc atttatttct cacttttccg cctcatcatc
    11581 gcgcgttaat ttcttttcat aatcgcgttt tacaatatcc agcgcgcgca gcacggtgcg
    11641 ggctgggatc tgattttctt ccagtagcac aatcaaatcg acggccagtt tgacatcgtc
    11701 aggggcattt tccagtgaca tactctctcc attgttagcg ggttaaacgc gctaacctgt
    11761 tttcgatttt atccagcgca tggcggcagc gtgccaggcg cgcttcatag gcttccactt
    11821 cacgatgcag cgtctgctgt tccacgagat cggtcgctcg cgccagacgg gctcgacgtt
    11881 cggcaaccat ctcacgtagc cgccgctcaa aatcctgatg ctgaatacgt ttacgctgcc
    11941 agcgggcaat tttcggtggc gcactgtccc actcgcgcaa tgaccaggcg gaggcttcac
    12001 gcgcgatggc ttccagttgc gtcgccagat gttccgccag ccaggccact tgcggcagtt
    12061 gctgttgctc aactgcatga cgaagcgcag ccagattatc gcccgcctca tcgagacaag
    12121 cctgcagtgt tgtcgcacgg gtctgaaaaa ggtgcctgtc aaaacgggca cttagcgtgg
    12181 caaattgcgc taccggggca caacgctgac gcagcgtggc gagctgtcct tccagttttt
    12241 ccagcagcag ggcggttttc acgataagac ctcaatgaaa atgataattg ttatgctaaa
    12301 gtagccactc tgtaagctga caacactcaa tatgcaacga atcattttaa tcatcattgg
    12361 ctggctggcg gtagtgctgg gtacgctggg cgtggtatta ccggtattac cgacgacgcc
    12421 gtttatcctg ctggcggcct ggtgctttgc ccgttcttcc ccgcgctttc acgcctggtt
    12481 gctgtaccgc tcatggtttg gcagctatct acgtttctgg cagaaacatc atgcgatgcc
    12541 gcgcggcgca aaaccacggg cgattttgct tattttgatt acgtttacga tttcgctgtg
    12601 gttcgtccag atgccgtggg tgcgcatcat gttgctggta attctcgcct gtttgctttt
    12661 ttatatgtgg cgaattccgg tgattgatga aaagcaagaa aagcactgaa gcacaacaat
    12721 cgcagttgca attattgcgt acagccagta cattctggcg ttttcgagca caggcgcagg
    12781 cggtcaaagg ttaaacaact gttacttttg atacgttcaa aacgcgccgt gagtaccacc
    12841 gtaacaagca ggcatacact tatgaccgcg actgcacagc agcttgagta tctcaaaaat
    12901 agcatcaaaa gcattcagga ctacccaaaa cccggcattc ttttccgcga tgtcaccagc
    12961 ttactggaag acccgaaagc ttacgctctc agcatcgact tgctggttga gcgttacaaa
    13021 aatgcgggca ttaccaaagt ggtcggcacc gaagcgcgtg gcttcttgtt tggcgctccg
    13081 gtagctctgg gtctgggcgt tggctttgta ccggtccgta aaccgggcaa actgccgcgt
    13141 gaaaccatca gtgaaactta cgacctggaa tacggcaccg atcagctgga aatccacgtt
    13201 gatgccatca aaccgggcga taaagttctg gtggtggacg atctgctggc aaccggcggc
    13261 actatcgaag cgaccgttaa actgatccgt cgtctgggtg gtgaagtagc tgacgctgcg
    13321 ttcattatca acctgttcga tctcggcggc gaacagcgtc tcgaaaaaca gggcattacc
    13381 agctacagcc ttgtcccgtt cccgggccat taattatcgt cagtcttgcg ctgctcacgc
    13441 tacgggcagc acaagatgtg cattcagcct cgccgttctg acggggctgt gttagcatta
    13501 ccccttcgtg aatccacctt ccagcgtttc agagcctgcc aatgagttat caggtcttag
    13561 cccgaaaatg gcgcccacaa acctttgctg acgtcgtcgg ccaggaacat gtgctgaccg
    13621 cactggcgaa cggcttgtcg ttagggcgta ttcatcatgc ttatcttttt tccggcaccc
    13681 gtggcgtcgg aaaaacctct atcgcccgac tgctggcgaa ggggctaaac tgcgaaactg
    13741 gcattaccgc gacgccgtgc ggcgtgtgcg ataactgtcg tgaaatcgag caggggcgct
    13801 ttgtcgatct gattgaaatc gacgccgcct cgcgcaccaa agttgaagac actcgtgacc
    13861 tgctggataa cgtccagtac gcgccagcgc gtggtcgttt taaagtttat ctgatcgacg
    13921 aagtgcatat gctgtcgcgc cacagcttta acgcactgtt aaaaaccctt gaagaaccgc
    13981 cggagcacgt caagtttctg ctggcgacga ccgatccgca gaaattgccg gtgacgattt
    14041 tgtcacgctg tctgcaattt catctcaaag cgctggatgt cgagcaaatt cgccatcagc
    14101 ttgagcacat cctcaacgaa gaacatatcg ctcacgagcc acgggcgctg caattgctgg
    14161 cacgcgccgc tgaaggcagc ctgcgagatg ccttaagtct gaccgaccag gcgattgcca
    14221 gcggtgacgg ccaggtttca acccaggccg tcagtgcgat gctgggtacg cttgacgacg
    14281 atcaggcgct gtcgctggtt gaagcgatgg tcgaggccaa cggcgagcgc gtaatggcgc
    14341 tgattaatga agccgctgcc cgtggtatcg agtgggaagc gttgctggtg gaaatgctcg
    14401 gtttgttgca tcgtattgcg atggtacaac tttcgcctgc tgcacttggc aacgacatgg
    14461 ccgccatcaa gctgcggatg cgtgaactgg cgcgcaccat accgccgacg gatattcagc
    14521 tttactatca gacgctgttg attggtcgca aagaattacc gtatgcgcca gaccgccgta
    14581 tgggcgttga gatgacgctg ctgcgcgcgc tggcgttcca tccgcgtatg cctctgcctg
    14641 agccagaagt gccacgccag tcctttgcgc ccgttgcgca aacggcagta atgacgccaa
    14701 cacaggtgcc gccgcaaccg caatcagcgc cgcagcaggc accgactgta ccgctcccgg
    14761 aaaccaccag ccaggtgctg gcggcgcgcc agcagttgct gcgcgtgcag ggagcaacca
    14821 aagcaaaaaa gagtgaaccg gcagccgcta cccgcgcgcg gccggtgaat aacgctgcgc
    14881 tggaaagact ggcttcggtc accgatcgcg ttcaggcgcg cccggtgcca tcggcgctgg
    14941 aaaaagcgcc agccaaaaaa gaagcgtatc gctggaaggc gaccactcca gtgatgcagc
    15001 aaaaagaagt ggtcgccacg ccgaaggcgc tgaaaaaagc gctggaacat gaaaaaacgc
    15061 cggaactggc ggcgaagctg gcggcagaag ccattgagcg cgacccgtgg gcggctcagg
    15121 tgagtcaact ttcgctacca aaactggtcg aacaggtggc gttaaatgcc tggaaagagg
    15181 agagcgacaa cgcagtatgt ctgcatttgc gctcctctca gcggcatttg aacaaccgcg
    15241 gcgcacagca aaaactggct gaagcgttga gcacgttaaa aggttcaacg gttgaactga
    15301 ctatcgttga agatgataat cccgcggtgc gtacgccgct ggaatggcgt caggcgatat
    15361 acgaagaaaa acttgcgcag gcgcgcgagt ccattattgc ggataataat attcagaccc
    15421 tgcgtcgatt cttcgatgcg gagctggatg aagaaagtat ccgccccatt tgatcgtaag
    15481 cacagcttac gttcgtcatc cttaacgtga ttgagagaga aacctatgtt tggtaaaggc
    15541 ggtctgggta acctgatgaa gcaagcccag cagatgcaag aaaaaatgca gaaaatgcag
    15601 gaagagatcg cgcagctgga agtcaccggc gaatctggcg caggtctggt aaaagtgacc
    15661 atcaacggtg cacacaactg ccgtcgcgta gagatcgacc cgagcctgct ggaagacgac
    15721 aaagagatgc tggaagacct ggtggctgca gcattcaacg acgcagcacg tcgtattgaa
    15781 gaaacgcaga aagaaaaaat ggcctctgta tcctccggaa tgcagctgcc gcctggcttt
    15841 aagatgccgt tctgatgcaa accagcccgc tgttaacaca gcttatggaa gcactgcgct
    15901 gcctgccggg cgttggcccg aagtcggcgc agcgtatggc gttcacgctg cttcagcgcg
    15961 atcgtagcgg cgggatgcgt ctggcgcagg cgctcacccg ggcgatgtcg gaaatcggcc
    16021 actgcgccga ttgccgcacc ttcaccgaac aggaagtctg taacatctgt tcgaatccgc
    16081 gtcgtaagga aaacggtcaa atctgcgtgg tggagagtcc ggcggacatc tacgccattg
    16141 agcagacggg gcagttttca ggccgttatt ttgtgttgat ggggcacctg tcaccgctgg
    16201 acggcatcgg tccggatgat atcgggcttg atcgtctgga acagcgtctg gcagaggaaa
    16261 aaatcactga agtgatcctc gccaccaacc ccacggttga aggtgaagct accgctaact
    16321 acattgccga gctttgtgcg caatatgacg tggaagccag ccgaatcgct catggcgtac
    16381 cggttggcgg cgaactggaa atggtcgatg gcaccacgct gtcacactcc cttgccgggc
    16441 gtcataagat tcgtttttaa gcaaacgaga gcaggatcac ctgctctcgc ttgaaattac
    16501 tctcccttgt ccccatctct ccctcatcct gtttttaacc ttaaaatggc attattgagg
    16561 tagacctaca tgaaaggaca agaaactcgt ggttttcagt cagaagtgaa acagcttctg
    16621 cacctgatga tccattctct ctattccaat aaagaaatct tcctgcgtga gcttatctct
    16681 aacgcctccg atgcggcgga caagctgcgt ttccgcgcgc tttctaaccc ggacctgtac
    16741 gaaggtgatg gtgaactacg cgttcgtgtc tctttcgata aagacaagcg cacgctgacc
    16801 atctccgata acggcgtggg gatgacctgc gacgaagtga ttgaccatct ggggactatc
    16861 gctaaatccg gcaccaaatc attcctcgaa tccctgggtt ctgaccaggc gaaagacagc
    16921 cagctgatcg gtcagtttgg tgttggtttc tactctgcgt ttatcgtggc tgacaaagtg
    16981 accgtgcgta ctcgcgcggc aggcgaaaaa ccagaaaacg gcgtcttctg ggaatcggct
    17041 ggcgaaggtg aatacaccgt tgccgacatc actaaagaag atcgtggtac tgaaatcacc
    17101 ctgcatctgc gtgaaggcga agacgagttc ctcgatgact ggcgtgtgcg ttccatcatc
    17161 agcaaatatt ccgaccatat cgcgctgccg gtagagatcg aaaaacgcga agagaaagac
    17221 ggcgaaaccg ttatctcctg ggagaaaatc aacaaagcgc aggcgctgtg gactcgtaac
    17281 aagtcggaaa tcaccgatga agagtacaaa gagttctaca aacacatcgc ccacgacttt
    17341 aacgatccgc tgacctggag ccataaccgc gttgaaggta agcaggagta caccagcctg
    17401 ctgtacattc cgtctcaggc accgtgggat atgtggaacc gcgatcataa acacggtctg
    17461 aaactgtatg ttcagcgtgt gttcatcatg gacgacgcag aacagttcat gccgaactat
    17521 ctgcgcttcg tgcgtggtct gattgactcc agcgatctgc cgctgaacgt ttcccgtgaa
    17581 atcctccagg acagcacgga aacgcgtaac ctgcgcaatg cgctgaccaa gcgtgtgctg
    17641 caaatgctgg aaaaactggc gaaagacgac gcggaaaaat accagacctt ctggcaacag
    17701 tttggcctgg tactgaaaga aggtccggcg gaagatttcg ctaaccagga agcgatcgcc
    17761 aaactgctgc gttttgcttc tacccatacc gattcttctg cgcagaccgt gtctctggaa
    17821 gactacgttt cccgcatgaa agaagggcag gagaaaatct actatatcac cgcagacagt
    17881 tacgcggcag cgaagagcag cccgcacctg gaactgctgc gtaagaaagg catcgaagtt
    17941 ctgctgcttt ccgaccgcat cgatgagtgg atgatgaact atctgactga gttcgacggt
    18001 aaaccgttcc agtctgtgtc taaagttgac gagtcgctgg aaaaactggc tgacgaagtt
    18061 gatgagagcg cgaaagaagc ggagaaagca ctgactccgt tcatcgaccg tgtgaaagcc
    18121 ctgctcggcg agcgcgtgaa agatgtccgt ctgactcacc gtctgaccga tacgccagcg
    18181 atcgtttcga ccgacgcgga cgaaatgagc actcagatgg cgaaactgtt cgctgcggcg
    18241 ggccagaaag tgccagaagt gaaatacatc ttcgaactga acccggatca cgtactggtg
    18301 aaacgtgcgg cggatactga agatgaagcg aagttcagcg agtgggtaga actgctgctg
    18361 gatcaggcgc tgctggcaga acgcggtacg ctggaagatc cgaacctgtt tattcgtcgt
    18421 atgaaccagc tgctggtttc ctgatgtcat gccggatgac cttcgtgtca tccggcattt
    18481 ttcttttctt catctgcact ttccgcaaat tatcttgcca ttaaccgttt cagcctcagg
    18541 tgcctttctt gaggcaatcg cctgttggtg gtatcgttta tcgctttttc aaaaaattcg
    18601 acacatttta aggggatttt cgcaatgcgt atcattctgc ttggcgctcc gggcgcgggg
    18661 aaagggactc aggctcagtt catcatggag aaatatggta ttccgcaaat ctccactggc
    18721 gatatgctgc gtgctgcggt caaatctggc tccgagctgg gtaaacaagc aaaagacatt
    18781 atggatgctg gcaaactggt caccgacgaa ctggtgatcg cgctggttaa agagcgcatt
    18841 gctcaggaag actgccgcaa cggtttcctg ttggacggct tcccgcgtac cattccgcag
    18901 gcagacgcga tgaaagaagc gggcatcaat gttgattacg ttctggaatt cgacgtaccg
    18961 gacgaactga ttgttgaccg tatcgtaggc cgccgcgttc acgcgccgtc tggtcgtgtt
    19021 tatcacgtta aattcaatcc gccgaaagta gaaggcaaag acgacgttac cggtgaagaa
    19081 ctgactaccc gtaaagacga tcaggaagag accgtacgta aacgtctggt tgaataccat
    19141 cagatgactg caccgctgat cggctactac tccaaagaag cggaagcggg taacaccaaa
    19201 tacgcgaaag ttgacggcac caagccggtg gctgaagttc gcgcagctct ggaaaaaatt
    19261 ctcggctaat caagccgcag cagatctgct ttcgggcagg tctgctcatc ctcctcaggc
    19321 aatacctctt acagcaatta gttcttcttc ttcacttttc cgctacaatt atcaacaagt
    19381 tgaatcgata agaggcggta atgcgtcaaa ctaaaaccgg tatcctgctg gcaaacctgg
    19441 gtacgcctga tgcccccaca cctgaagcgg taaaacgcta tctgaaacaa tttttaagcg
    19501 acagacgcgt ggttgatacc tcacggttgt tatggtggcc gttgctgcgc ggcgtgattt
    19561 tgccgctgcg ttcgccgcgt gtggcgaagc tgtatgcctc tgtctggatg gaaggtggct
    19621 cgccgctgat ggtttacagc cgccagcaac agcaggcgct ggcgcaacgt ttaccggaga
    19681 cgcccgtggc gctgggaatg agctacggct cgccatcact ggaaagcgcc gtagatgaac
    19741 ttctggcaga gcatgtagat catattgtgg tgctgccgct ttatccgcaa tactcctgtt
    19801 caacggtcgg tgcggtatgg gatgaactgg cgcgaattct ggcgcgcaaa cgtagcattc
    19861 cggggatatc gtttattcgt gattacgccg ataaccacga ttatattaat gcactggcga
    19921 acagcgtacg tgcttctttt gccaaacatg gcgaaccgga tctgctgttg ctctcttatc
    19981 atggcattcc ccagcgttat gcagatgaag gcgatgatta cccgcaacgt tgccgcacaa
    20041 caactcgcga actcgcttcc gcactggaga tggcaccgga aaaagtgatg atgacctttc
    20101 agtcgcgctt tggccgggaa ccctggctga tgccttatac cgacgaaacg ctgaaaatgc
    20161 tcggagaaaa aggcgtgggt catattcagg tgatgtgccc gggctttgct gcggattgtc
    20221 tggagacgct ggaagagatt gccgagcaaa accgtgaagt cttcctcggc gctggcggga
    20281 aaaaatacga atatattccg gcgcttaatg ctacaccgga gcatatcgaa atgatggcta
    20341 atcttgttgc agcctatcgc taaagaatcg agcaccgtcg cgtagagcat cgtcggcggt
    20401 tttcatcatc cgtgaataat gcaaaaaggc gtgcagcata ccggagtaga gtttgaactc
    20461 acaaggttgc tggtgtgccg ctaacgtctg gtaaagcaga cggctgtcat ccagcagtgg
    20521 atcgaactcc gccccggcaa taaaacaggg cggaacttcg cgagtgagat cattattaaa
    20581 caggcagtaa tatggcgact cgcggtccgc gtcgttgctt aaatacgcct cttcatacat
    20641 ctgcaaatcc tgttgcgtta agccatccca gacaccaccc aacagacgac gagtcacgga
    20701 atcccgtaat ccgtaaagcc cataccacag caaaacgccc gcaactttac cgcaatcgat
    20761 ctgtttatcg cgcaaccaca acgcactggc gagcgccagc atggcacctg cggaatcacc
    20821 agcaaaacca atgcgggaca tattgatttg ataatcctcc gcctgctggt ggaaataaca
    20881 acaggcagcc acaatttcct ctatcgcttg cggaaaacgc gcttcaggtg aaagggtgta
    20941 atcaatgcca atcaccgtac attggctgta gcttgccagc aggcgcatga tgcgatcgtg
    21001 ggtatcgaga ttgccgagaa taaaaccgcc tccatgcaaa taaaatagcg tcgccgggct
    21061 atctggctgc ggataaaaga tacgtgtttt cacctgccca tattttgttg gaaccctgta
    21121 agctctggtt gccatttctg gagcgcccac attccagaat cggcgctcaa gcgtgtaata
    21181 ctgtcgttgc tcagcaatcg ttcccgttgc aggccagggc ggtaaatccg gctgaagagt
    21241 attcacaacg gtcttcattt cagcagagat aaggtccaga acgggtaatt tgttttccgg
    21301 cttcatgcaa aaactccttt caaattacgt cattgtaagg aaccactgcc atgaaaatgc
    21361 gatcccgcct gctgatattg aaactggctg cgtctcgcgc gctcccgtca gattgtgtta
    21421 acattcgccg ctcagttaac cacccgtaaa aacaaccatg aaatttcccg gtaaacgtaa
    21481 atccaaacat tacttccccg taaacgcacg cgatccgctg cttcagcaat tccagccaga
    21541 aaacgaaacc agcgctgcct gggtagtggg tatcgatcaa acgctggtcg atattgaagc
    21601 gaaagtggat gatgaattca ttgagcgtta tggattaagc gccgggcatt cactggtgat
    21661 tgaggatgac gttgccgaag cgctttatca ggaactaaaa cagaaaaacc tgattaccca
    21721 tcagtttgcg ggtggcacca ttggtaacac catgcacaac tactcggtgc tcgcggacga
    21781 ccgttcggtg ctgctgggcg tcatgtgcag caatattgaa attggcagct atgcctatcg
    21841 ttacctgtgt aacacctcca gccgtatcga tcttaactat ctacaaggcg tggatggccc
    21901 gattggtcgt tgctttacgc tgattggcga gtccggggaa cgtacctttg ctatcagccc
    21961 gggccacatg aaccagctgc gggctgaaag tattccggaa gatgtgattg ccggagcctc
    22021 ggcactggtt ctcacctctt atctggtgcg ttgcaagccg ggtgaaccca tgccggaagc
    22081 aaccatgaaa gccattgagt acgcgaagaa atataacgta ccggtggtgc tgacgctggg
    22141 caccaagttt gtcattgccg agaatccgca gtggtggcag caattcctca aagaccacgt
    22201 ctctatcctt gcgatgaacg aagatgaagc cgaagcgttg accggagaaa gcgatccgtt
    22261 gttggcatct gacaaggcgc tggactgggt agatctggtg ctgtgcaccg ccgggccaat
    22321 cggcttgtat atggcaggct ttaccgaaga cgaagcgaaa cgtaaaaccc agcatccgct
    22381 gctgccgggt gctatagcgg aatttaacca gtatgagttt agccgcgcca tgcgccacaa
    22441 ggattgccag aatccgctgc gtgtctattc gcacattgcg ccgtacatgg gcgggccgga
    22501 aaaaatcatg aacactaatg gagcagggga tggcgcactg gcagcgttgc tgcatgacat
    22561 taccgccaac agctaccacc gtagcaacgt accaaactcc agcaaacata aattcacctg
    22621 gttaacttat tcatcgttag cgcaggtgtg taaatatgct aaccgtgtga gctatcaggt
    22681 actgaaccag cattcacctc gtttaacgcg cggcttgccg gagcgtgaag acagcctgga
    22741 agagtcttac tgggatcgtt aagttatcgt cggttcgtag gccagataag aagcgcctat
    22801 tatccggcaa ccaccgttta ccccgtcacc acctcacccg ccggtggcgt ttccagcagt
    22861 tccagcatag tacgggcaat ttcacgctca cccatcacta cctgattcgc accacgttcg
    22921 gtgatatacg ccacttcatc gtcataatgg gcgcgggcaa taatctcaat atccggattt
    22981 ttcgcgcggg cagatgccac aatctcgccc gcttcatagc cgttgggaat cgtcaggatc
    23041 agccattttg cacattccag atgcgccagt tgcataattt cttcgttcgc cgcattgccc
    23101 aatactgcgc ggaccccgcg ctcgcgcagc tcatcaacac gggttcgtga cgtctcaatc
    23161 accaccagcg gaatatcaga ggcgagcaat ttctccccca gcaggctgcc tacacgaccg
    23221 taacccacca gtagcgcatg gttgcaaata tccactggga tctgcttctc ttcttcgatt
    23281 gcctcttcca gcgtctgctc ttccagcgtt tcggtcttcg ccagatattt ttccagtagt
    23341 gcgaacagta ccgggttgag cataatcgac aggatcgccc ctgccagtac caggttttgt
    23401 ccggcctgcg gcagtaaatt taatgccatt cctagtcccg ccaggataaa cgcgaactca
    23461 ccaatctgcg ccaggctggc ggcgatggtt aacgccgtgc gctgggagtg accaaacaaa
    23521 cgcacaagga acagggccgc gaccgattta ccaaacagaa taattgccag cgtcgccagt
    23581 actgccagcg gttgctgaat cagaattaac ggatcaaaca acatcccgac ggagacaaaa
    23641 aacagcaccg caaacgcgtc gcgcaatggc agtgtatcgt gggcggcacg gtgactcagt
    23701 tcagactcgt tcagtaccat cccggcaaag aacgcaccga gtgcaaagga gacatcaaac
    23761 agctctaccg caccaaaggc aatccctaac gccagcgcca gcaccgacag ggtaaacagc
    23821 tcgcgggaac cggttgctgc gctgcgtgcc ataatccacg gcaccagacg gcgacctacc
    23881 agcatcataa tggcgataaa tgcgatcacc ttgccgatgg tgatccccat atcgactgca
    23941 agagtggcaa agcccacatc gccctgttcc atcattcctg ctaccgcggg tagcaatacc
    24001 agtgtcagga ccatcaccag gtcttccaca atcaaccaac cgatggcgat ttgcccacgc
    24061 tgactgtcaa ttaattgccg ttcttcaagt gcgcgcagta acaccacggt actggcggtg
    24121 gaaagacata agccgaacac gataccggtc attaacgacc agcccagcac ggcggagagc
    24181 gccataccca gcagcgtcgc cacggctatc tgggcgatcg cgccgggaat ggcgatggac
    24241 tttaccgcca tcaaatcctt gagcgaaaaa tgcaaaccga cgccgaacat cagcaaaata
    24301 acgcccagtt cagccagttc cggcgcaagc ttggtatcgg caacaaagcc cggagtgaat
    24361 ggtcctgcca gcacacccgc taacagatat cccaccagag gagaaatacg tagtttattg
    24421 gccagcatgc cgaggataaa ggcgagcaca aggccgccaa caatggtggt gataagcggg
    24481 gtggcgtgat gcattccgtc tccttttcct ggtggttatt gtccattttt ggccgggaaa
    24541 accaaaatta caggtaatag tttatgacaa tttcattgat gatgtttatg aataattgtt
    24601 gaattttgca gaaaaatgga attagctgca aaaaaagcac ggatcggaaa acagaaggcg
    24661 gtttaacaga ggaaaggctt atggcgactg taaaacgatg cagccaaagt ttgcctttgg
    24721 ctgcaatgaa atcagtcttt atgccggtta tcaggcagga atatggtcaa catccccaat
    24781 agtggcagga aagcacagat tttatagact aactctatgc tggtgtggtc ggcgataagc
    24841 cccagaactg ccgcccccag acctcccatg ccgaaagcaa aaccgaaaaa cagtccagaa
    24901 accataccga tacgtcccgg aagcagctcc tgggcgtaga ccagaatggc agagaatgct
    24961 gaagcgagga taaatccaat aatcaccgtt aaaacccccg tccagtgcag gctggcgtag
    25021 ggtaaaatca gcgtaaacgg cgcaacgccg aggatagagc cccaaatcac atatttccgt
    25081 ccaattttat cccctacagg cccgccaatc accgtacctg ccgcaacggc aaacaggaag
    25141 gcaaacaaat gaagctgagc attctggata gataatccga atttttgcat cagataaaag
    25201 gtgtaatagc tgctgatgct cgccatatag aaatatttcg agaaaatgag gattaacaga
    25261 atgctgaccg ccagaacaac tttattgcgc ggcagtggat tgataatcgt cgctttgggt
    25321 tttcctttat tcattcggtg ctgtgccgag taccaacggc tgatttgcgc caacaccacg
    25381 atcgccagca gtgccgcaag cacaaaccag gcaacgttgc ctttgccata aggcgcgata
    25441 atcaccgccg ccagcaaggg gcccagggaa ctgccaaagt tgccgccgac ctgaaagata
    25501 gattgcgcca ggccatgacg cccgccggaa gccatacggg ccacgcgaga agattccgga
    25561 tgaaagaccg atgaaccggt accgaccagc gccgctgcca gcagaactgc gccaaaactg
    25621 cccgccagcg ccagcagcac cagaccactt aaggtaaaac acatgccaat cggcaacgac
    25681 cacggcatcg gatatttatc ggtccagtag ccgaccaccg gttgcagtag cgaagaggcg
    25741 agctggaagg tgagggttat catgccaatc tgcataaatg tcagagaaaa ttctgactga
    25801 agcagtggat aaatcgccag aatcagcgat tggatcatgt cgttcagcag atgtgagagg
    25861 ctgatagcac ctaaaatacc aaacgatgtt cgggccttag tcgttgacgc agccgcgccc
    25921 gccacaggct gggtttgttc actcattgcc ataggaaagt cactttttca gggttgcgat
    25981 gtaaagaatg atcttatttg tgattattac ccgactaaca tacctgtatg cgccgcctga
    26041 aggaagtctc aacgcggaat acagaatttc taatctggat gcagatttat cttcaccgga
    26101 cgcagacttg tttatgatgt cgcgtcatac tatttttcaa cacgttgaaa tcaggtcagg
    26161 gagagaagta tgaaattatt gcagcggggc gtggcgttag cactgttaac cacatttaca
    26221 ctggcgagtg aaactgctct ggcgtacgag caggataaaa cctacaaaat tacagttctg
    26281 cacaccaatg atcatcatgg gcatttttgg cgcaatgaat atggtgaata tggtctggcg
    26341 gcgcaaaaaa cgctggtgga tggtatccgc aaagaggttg cggctgaagg cggtagcgtg
    26401 ctgctacttt ccggtggcga cattaacact ggcgtgcccg agtccgactt acaggatgcc
    26461 gaacctgatt ttcgcggtat gaatctggtg ggctatgacg cgatggcgat cggtaatcat
    26521 gaatttgata acccgctcac cgtattacgc cagcaggaaa agtgggctaa gttcccgttg
    26581 ctttccgcca atatctacca gaaaagtacc ggcgagcgcc tgtttaaacc atgggcgctg
    26641 tttaagcgtc aggatctgaa aattgccgtt attggcctga cgacggatga cacagccaaa
    26701 attggtaaaa cggcatctgg attgtgcagg cgcatgagtg gggcaaatac gtgggacggg
    26761 ctgattttga gtttcgtaat ggcgaaatga aaatggttaa ctaccagctg attccggtga
    26821 acctgaagaa gaaagtgacc tgggaagacg ggaaaagcga gcgcgtgctg tacacccctg
    26881 aaatcgctga aaaccagcaa atgatctcgc tgttatcacc gttccagaac aaaggcaaag
    26941 cgcagctgga agtgaaaata ggcgaaacca atggtcgtct ggaaggcgat cgtgacaaag
    27001 tgcgttttgt acagaccaat atggggcggt tgattctggc agcacaaatg gatcgcactg
    27061 gtgccgactt tgcggtgatg agcggaggcg gaattcgtga ttctatcgaa gcaggtgata
    27121 tcagctataa aaacgtactg aaagtgcagc cattcggcaa tgtggtggtg tatgccgaca
    27181 tgaccggtaa agaggtgatt gattacttga ccgccgtcgc gcagatgaag ccagattcag
    27241 gtgcctaccc gcaatttgcc aacgttagct ttgtggcgaa agacggtaaa ctgaacgacc
    27301 tgaaaatcaa aggcgaaccg gtcgatccgg cgaaaactta ccgcatggcg acattaaact
    27361 tcaatgccac cggcggggat ggctatccgc gccttgataa caaaccgggc tatgtgaata
    27421 ccggctttat tgatgccgaa gtgctgaaag cgtatatcca gaaaagctcg ccgctggatg
    27481 tgagtgttta tgaaccgaaa ggtgaggtga gctggcagta atccgaaagt gccggatgtt
    27541 cgcatccggc acaatgctta atcgcgacgg gcgatatcag caaatttggc atcgagtatc
    27601 tttgccaggt cgcttgccgc cagttcgata tccagaccgc gcttgccgcc ggaaacataa
    27661 atagtggcaa attcttgtgc gggggcgtcg ataatcgttg gcagacgttt tttctgcccc
    27721 agtgggctaa tccctccaac cagatatccc gtcgaacgct gcgcgaccat cggatcggcc
    27781 atctcaactt tcttggcacc cagcgctttt gctacttttt taagatccag ttgacctgcg
    27841 accggcgtaa cggccacggc aaggtgtttc atatcaccgt tcactgcgac cagcagcgtt
    27901 ttgtagacct gatccggatt caaacctaat tttttgacga cttcatcgcc aaaattggtt
    27961 tcagccggat cgtgctcgta ggtatggatt tgaaacgaaa tcttgttttt ttcgagtaat
    28021 ttaactgcgg gtgtcatggt aattttcctg ccttaaacaa aaaaacgaag caagcatacg
    28081 cctgatggcg gtccaaaaaa aagagtcatc ttgcctaaga gtattggcag gatggtgaga
    28141 ttgagcgaca atcgagttac accgtcgctt aaagtgacgg cataataata aaaaaatgaa
    28201 attcctcttt gacgggccaa tagcgatatt ggccattttt ttagcgcaac atctgcggca
    28261 aattcccttc tccatacagg tgtagtgcac cgactgcgac cacatagcgc cccggcggca
    28321 tggcgcgtaa tttatcccgc caggcgagat ttcgctgatg catcagcaca tcgtacagcg
    28381 actgactgaa cgtattgggc agcgttattt cattattttg cggcggcgca ttcaaccacc
    28441 agctcatcat ttgttgcagc aaccgtgcat tggtgtgcca gtgggtcagc gtgtcgtcca
    28501 gcagcgccag tccgttgtca gggagctgga gcaacatggc aatctggttt tcagcccctt
    28561 ccagttcaat cacgggttta tgttgttgct tcgccgcctg caatagctgg taatcgatac
    28621 cgtattctgc ccgcagcccc agtttttgtg cctgcgtcgc ctgaagaacc atcgcgattt
    28681 gccacagcgg ttgagtagaa aagagcgacg gagaaatgcc catctcctgg ctaatatgct
    28741 gcagattttg gagttgctcc tcgctaatgc gctcttccag cgtctcgcag gcaggcaaat
    28801 tagcaaaagg cgtatcgctg gtggaaacat ccgcctcgac gatcagcgca tcggcgtttt
    28861 tgagcttttt gagcaaacgg gtgggcaggg gagccatatc gtggctaccc atatgaatac
    28921 tgccgatcag atgaaaatgg cgattgccgg gaagagagat atcgatagca ggccaggtgt
    28981 aatgattacc gcgcagcgcg gcccaaagcg tttttacccg gtacaacaga tccatacgac
    29041 ctccctttgt gaaatatcat gctagcgcgc ggggagggat ggcgcaaatg gagttgattg
    29101 ttgcacttgt acacagtgct tgtgtgcatt acaatgatta aataatggac aaggagatga
    29161 gagaggaatg gctcaaaaga aaaatataag gagttttcgt gatgcctggt tggctgattt
    29221 tttgtccatt caacgccaca tcgaaagatc cctgctgaaa tccataccac gttatcacga
    29281 aagttggaca tcattaatgc tgccacttct catcgggatt tacgctcccc tccggggaac
    29341 cgatacgaag agttatccgg gaaattacag gaatactcct ccattagagt gaataaacag
    29401 taccgactga tttttaaatg ggttaatggc aaggccgaag aattgttttt agacccacac
    29461 aactactaaa acatagctgt cgtattgacg agaaaggtaa aactatgaaa caggcaacaa
    29521 gaaaaccgac gacacccgga gatattcttc tctatgaata tctggaaccg ctcgatttga
    29581 aaatcaatga gttagcagag ttgctgcatg ttcatcgtaa tagcgtcagt gcactgatca
    29641 ataacaatcg taaactcacc actgagatgg cattccgtct ggcgaaagtt tttgatacca
    29701 cagtcgattt ttggctaaac ctccaggcgg cagttgatct ttgggaagtt gaaaacaaca
    29761 tgcgtaccca ggaagaattg ggacggattg aaacagtggc tgaatatttg gcacgccgtg
    29821 aagagcgtgc aaaaaaggtc gcgtagaata atttccctat aacggatgcg ccaccctaaa
    29881 gcagcgcatc cgcaatgatg tacttattcc ttcggtttaa accgcagcaa tcggttggcg
    29941 ttactcacta cggtgatcga cgagagcgcc attgccgctc cggcaaccac cgggttaagc
    30001 agtgttccag tgaacggcca caaaataccg gcggcgaccg gaataccaat gctgttgtag
    30061 ataaacgcac cgagcaggtt ctgcttcatg ttgcgcagcg ttgcgcggga aattgcgagc
    30121 gcatccgcaa cacccatcag gctatggcgc atcagggtaa tcgccgcggt ttcaatggca
    30181 acatcactgc cgccacccat cgcaataccg acatccgcct gagccagcgc tggcgcatcg
    30241 ttaatgccgt ctcccaccat tgccacctga cgtccttcac tttgcagacg tttgatcgct
    30301 tcggctttac cgtctggcag cacgccggct atcacctcat caatccctgc ttctttagcg
    30361 atcgcattgg cggtggtcgg gttatccccg gttagcatca ccaggcgata tcccgcttta
    30421 tgcaggcgtt gcagcgccgc cacgctatca ctacgcaacg gatcgcgtac agccagcagg
    30481 gctactgctt tcccgtcaac cgccagcagc acaggcgttg ccccttgcga tgcctgagct
    30541 gaaatatctg cttcaattgc tttggtattg acctgttggt catttaacaa cgcctgattg
    30601 cccagcaata acgcatgacc ttcagcttca ccgctcacgc ccagcccgcg caatgtgcgg
    30661 aaaccgttga cctgcggtag ctgcatatca cctgctttat cgaggatcgc ccgtgccaga
    30721 gggtggctgg aaccttgctc cagcgccgcc gccagacgca atgcttgcgc ttcatcaata
    30781 tcagcaaatg tttttactgc gacaacctgc ggcttccctt cggtcagcgt cccggtttta
    30841 tcgaacacta cggtgtcgag cgtactggcg cgttgcagcg cgtcggcgtc ccgtaccagc
    30901 acgccaaact cagccgcccg cccgacgccg gaaataatcg acatcggcgt cgccagtccc
    30961 agcgcacacg gacaggcaat aatcagcacc gtggtggcaa tcaccagggt atagacaatc
    31021 tgcggtgccg gaccaaagaa ataccagatt gccgcactga tcagcgcaat caccaccact
    31081 accggcacaa atacggctga gattttatcc gccagctgac cgatttctgg cttactgctc
    31141 tgggcctggc gcaccatacg aataatccgc gacagcgtag tatggctgcc aaccgcactg
    31201 gcgcgaaaca gcacgctacc gtcctgcacc actgtcccgg catggacgct atcgccttcg
    31261 cctttttgct gcgggatagg ttcgcccgtc agcatcgctt catccagcca tgcttcgccc
    31321 tgggtaatct cgccatctac tggcacgcga tcgccggtcg tcaggcgcag caacatacct
    31381 ggttgcactt ccgccagcgg cacgcttttt tcaccttcgt cagtaaccag gcgcgccgtc
    31441 ggcggagtta aatcgagtaa tttctccagc gcctttgaag aacgctgacg tgcgcgcgct
    31501 tccagcatat gaccgagatt aatcagaccg ataatcatcg cactggcttc atagtaaaga
    31561 tgccgtgctt ccatcgggaa ccactgcggc cacaggttga cgctcatcga atagagccac
    31621 gcaacacccg ttcccagcgc caccagcgta tccatcgtcg ccgcaccgtt cagcaggctt
    31681 ttccatgcgc tgcggtaaaa atgcccgccg gcgaaaacca ttaccgccag ggttatcagg
    31741 ccgataacta accacaggct gcggttgtca gcggtgacca tcatgttatc gccgatcatc
    31801 ccccagacca tcaccgggat acccaccacc agcgcgacaa ttgcctgcca gcggaagcgc
    31861 ttcatcgtag cgacggcggt ttcttgctgg cgctcgcggc gtttagcgtc atcttcaatc
    31921 gcttccgcgc cgtagcccgc tttttccacc gcctgcacta aatcttgtgg ggaggcactg
    31981 cccatcacca gcgcagtacg ctccgccagg tttacccgtg cctgagtgac gcccggtacg
    32041 ctttgcaacg cattttgtac gcgggtgaca cagctggcgc agctcatgcc gctcagcagc
    32101 aactgctggc tgtcatcgtc atcggcggtc gctgccggaa gcgcctcaga aaccgctgtc
    32161 agtgcttccg acgggattga tgactccgcc agcggtttag cctttgggtg gcttacagat
    32221 gcgtcataac ccgcttgttt gatggtttcg atcagctgtt ctgcactggc agtcccggta
    32281 acgtgcgctt cagtgataga cacatccgcc tgttcaacat ccgggcgctg ttcgagactt
    32341 tctttcacgc gtttaacgca gtgaccgcag gacaggccat ccagggtcag gtcgatagtt
    32401 tgtgacataa aacactcctt taagacagtt ttgactggct gtgataaagg ttaaaccttc
    32461 cagcaagggg aaggtcaaga aattaataaa ccaggcgggt aaaagtccgt aaagattaaa
    32521 aaatcggctc gatttgcatc aggattagac atttatctct ttgttttcct gtagttaagt
    32581 tgcgggtgct aagttaaatc aggatgcatg aaaatcggca ccggggtgag gaattacctc
    32641 ccgcatctat aaaaaggagt taacaaaaga tgttagatgc aaacaaatta cagcaggcag
    32701 tggatcaggc ttacacccaa tttcactcac ttaacggcgg acaaaatgcc gattacattc
    32761 cctttctggc gaatgtccct ggtcaactgg cggcagtggc tatcgtgacc agcgatggca
    32821 acgtctatag tgcgggtgac agtgattacc gctttgcgct ggaatccatc tcgaaagtct
    32881 gtacgttagc ccttgcgtta gaagatgtcg gcccgcaggc ggtacaggac aaagttggcg
    32941 ctgacccgac cggattgccc tttaactcag ttatcgcctt agagttgcat ggcggcaaac
    33001 cgctttcgcc actggtaaat gctggcgcta ttgccaccac cagcctgatt aacgctgaaa
    33061 atactgaaca acgctggcag cgaattttac atatccaaca acaactggct ggtgagcagg
    33121 tagcgctctc tgatgaagtc aaccagtcgg aacaaacaac caacttccat aaccgggcca
    33181 ttgcctggct gctgtactcc gccggatatc tctattgtga tgcaatggaa gcctgtgacg
    33241 tgtacacccg tcagtgctcc acgctcatca atactattga actggcaacg cttggcgcga
    33301 cgctggcggc gggtggtgtg aatccgctga cgcataaacg cgttcttcag gccgacaatg
    33361 tgccgtacat tctggccgaa atgatgatgg aagggctgta tggtcgctcc ggtgactggg
    33421 cgtatcgcgt tggtttaccg ggcaaaagcg gtgtaggtgg cggtattctg gcggtcgttc
    33481 ctggcgtgat gggaattgcc gcgttttcac caccgctgga cgaagaaggc agcagtgttc
    33541 gcggtcaaaa aatggtggca tcggtcgcta agcaactcgg ctataacgtg tttaagggct
    33601 gatcattatg aacacggaag gtaataacgg taacaaaccc ctcggtctat ggaacgtcgt
    33661 tt
//
LOCUS       DAZFFE010000041        33552 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFFE010000041 DAZFFE010000000
VERSION     DAZFFE010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.041"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            101..679
                     /locus_tag="ACHJ69_002166"
     CDS             101..679
                     /locus_tag="ACHJ69_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO5015151.1"
                     /translation="MKDYSHVKKTLLSMFATALLSGVAFNALADDANQGSGKITFKGE
                     VIDALCSIAPGDEDQTINLGEVADTVLKSGQKSLPVDVTIHLQDCILSDGTNTVDKVK
                     ITFSSASVDATDSNLLKNTLEGNIGGATDVGVRLVKSDNTNVTLGTPITINFPTTNSY
                     QELNFKARMESLGRTATPGNVQAQANYVLDYK"
     gene            766..3288
                     /locus_tag="ACHJ69_002167"
     CDS             766..3288
                     /locus_tag="ACHJ69_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5015152.1"
                     /translation="MNNKNTFSRDKLSHAIKNALSGVVCSLLFVLPVHAVEFNVDMID
                     AEDRENIDISRFEKKGYIPPGRYLVRVQINKNMLPQTLILEWVKADNESGSLLCLTKE
                     NLTNFGLNTEFIESLQNIAGSECLDLSQRQELTTRLDKATMILSLSVPQAWLKYQATN
                     WTPPEFWDTGIAGFILDYNVYASQYAPHHGDSTQNVSSYGTLGFNLGAWRLRSDYQYN
                     QNFADGRSVNRDSEFARTYLFRPIPSWSSKFTMGQYDLSSNLYDTFHFTGASLESDES
                     MLPPDLQGYAPQITGIAQTNAKVTVAQNGRVLYQTTVAPGPFTISDLGQSFQGQLDVT
                     VEEEDGRTSTFQVGSASIPYLTRKGQVRYKTSLGKPTSVGHNDINNPFFWTAEASWGW
                     LNNVSLYGGGMFTADDYQAITTGIGFNLNQFGSLSFDVTGADASLQQQNSGNLRGYSY
                     RFNYAKHFESTGSQITFAGYRFSDKDYVSMSEYLSSRNGDESIDNEKESYVISLNQYF
                     ETLELNSYLNVTRNTYWDSASNTNYSVSVSKNFDIGDFKGISASLAVSRIRWDDDEEN
                     QYYFSFSLPLQQNRNISYSMQRTGSSNTSQMISWYDSSDRNNIWNISASATDDNIRDG
                     EPTLRGSYQHYSPWGRLNINGSVQPNQYNSVTAGWYGSLTATRHGVALHDYSYGDNAR
                     MMVDTDGISGIEINSNRTVTNGLGIAVIPSLSNYTTSMLRVNNNDLPEGVDVENSVIR
                     TTLTQGAIGYAKLNATTGYQIVGVIRQENGRFPPLGVNVTDKATGKDVGLVAEDGFVY
                     LSGIQENSTLHLTWGDNTCEVTPPNQSNISESAIILPCKTVK"
     gene            3304..4044
                     /locus_tag="ACHJ69_002168"
     CDS             3304..4044
                     /locus_tag="ACHJ69_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5015153.1"
                     /translation="MKSPLITTGMICLLGICNFAQATVSPDRTRIIFNASNKSATVRL
                     TNQSKIDPYLAQSWIEDASGKKTRDYISTLPPMERIEPDEQIQIRLMALASLNDLPQD
                     RETLFYYNVREIPPRAKEQNVMQIAMQSRLKLFWRPKAIELKEGEMIPLQKVTITRTA
                     AGLTLNNPTPYHITVGYIGTNGKTLMPGADSIMVAPFTSATQHLSSLPSTFQLGFVAD
                     YGGLEMFKVECNSIQSLCQSSPAKKGKI"
     gene            4041..5090
                     /locus_tag="ACHJ69_002169"
     CDS             4041..5090
                     /locus_tag="ACHJ69_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFO5015154.1"
                     /translation="MITLFRLLAILCLFFNVSAFAVDCYQDGYRGTTLINGDLPAFKI
                     PENAQPGQKIWESGDINITVYCDNAPGWTTDNPSENIYAWIKLPQINSADMLNNPYLT
                     FGVTYNGVDYEGINERIDTHACLDKYEQYYNGYYYDPVCNGSTLQKNVTFNAHFRVYV
                     KFKSRPAGDQTVNFGIVNVLQFDGEGGANMSSNAKNLRYVITGLDNISFLDCSVDVRI
                     FPESQIVNFGQIAANSIATYRPKAAFSVSTIKDVAADCTEQFDVATSFYTTDTLHDDT
                     HLEMGNGLLMRITDQKTQEDIKFNQYKRFTTYIPGQTGAMVTRDYQAELSQKPGETLV
                     YGPFKKDLIVKINYN"
     gene            complement(5144..5797)
                     /gene="ribB"
                     /locus_tag="ACHJ69_002170"
     CDS             complement(5144..5797)
                     /gene="ribB"
                     /locus_tag="ACHJ69_002170"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO5015155.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITDDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(5901..6049)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6171..6470
                     /gene="ubiK"
                     /locus_tag="ACHJ69_002171"
     CDS             6171..6470
                     /gene="ubiK"
                     /locus_tag="ACHJ69_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO5015156.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNPVAELQSPPAIPPVDKAE"
     gene            complement(6505..6705)
                     /gene="glgS"
                     /locus_tag="ACHJ69_002172"
     CDS             complement(6505..6705)
                     /gene="glgS"
                     /locus_tag="ACHJ69_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO5015157.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            6975..7604
                     /gene="yqiJ"
                     /locus_tag="ACHJ69_002173"
     CDS             6975..7604
                     /gene="yqiJ"
                     /locus_tag="ACHJ69_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO5015158.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACVTLWQSPLPNLF
                     VVPVSLLFTIIAVHYTGKVVAPWIPRDHSSAITEEEYVGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKTFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            7631..9292
                     /gene="yqiK"
                     /locus_tag="ACHJ69_002174"
     CDS             7631..9292
                     /gene="yqiK"
                     /locus_tag="ACHJ69_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO5015159.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLSGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGATGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSSLSSTTPVEEKAE"
     gene            9532..9591
                     /locus_tag="ACHJ69_002175"
     CDS             9532..9591
                     /locus_tag="ACHJ69_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5015160.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(9711..11144)
                     /gene="hldE"
                     /locus_tag="ACHJ69_002176"
     CDS             complement(9711..11144)
                     /gene="hldE"
                     /locus_tag="ACHJ69_002176"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO5015161.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(11192..14032)
                     /gene="glnE"
                     /locus_tag="ACHJ69_002177"
     CDS             complement(11192..14032)
                     /gene="glnE"
                     /locus_tag="ACHJ69_002177"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO5015162.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFLQD
                     SIIAHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSDGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPFDELNRARLTWAMDFADWPQLTGVLTAHMANVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLMLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSENCFTAERELVRASWQKWLVEE"
     gene            complement(14055..15356)
                     /gene="ygiF"
                     /locus_tag="ACHJ69_002178"
     CDS             complement(14055..15356)
                     /gene="ygiF"
                     /locus_tag="ACHJ69_002178"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO5015163.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPTDLASRVQPLFSTDFYREKWLVEVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAAFELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDLILLLAGYYDPVVAQ
                     DWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            15598..16218
                     /gene="ygiM"
                     /locus_tag="ACHJ69_002179"
     CDS             15598..16218
                     /gene="ygiM"
                     /locus_tag="ACHJ69_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO5015164.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            16282..17520
                     /gene="cca"
                     /locus_tag="ACHJ69_002180"
     CDS             16282..17520
                     /gene="cca"
                     /locus_tag="ACHJ69_002180"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO5015165.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLCFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVANWKEQRCPKSE"
     gene            complement(17702..18523)
                     /gene="bacA"
                     /locus_tag="ACHJ69_002181"
     CDS             complement(17702..18523)
                     /gene="bacA"
                     /locus_tag="ACHJ69_002181"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO5015166.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(18614..18982)
                     /gene="folB"
                     /locus_tag="ACHJ69_002182"
     CDS             complement(18614..18982)
                     /gene="folB"
                     /locus_tag="ACHJ69_002182"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO5015167.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            19087..19704
                     /gene="plsY"
                     /locus_tag="ACHJ69_002183"
     CDS             19087..19704
                     /gene="plsY"
                     /locus_tag="ACHJ69_002183"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO5015168.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(19717..20649)
                     /gene="ttdR"
                     /locus_tag="ACHJ69_002184"
                     /gene_synonym="dan"
     CDS             complement(19717..20649)
                     /gene="ttdR"
                     /locus_tag="ACHJ69_002184"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO5015169.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDAIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQIM"
     gene            20856..21767
                     /gene="ttdA"
                     /locus_tag="ACHJ69_002185"
     CDS             20856..21767
                     /gene="ttdA"
                     /locus_tag="ACHJ69_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO5015170.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLRVHADLTFENLSHTRSAL"
     gene            21764..22369
                     /gene="ttdB"
                     /locus_tag="ACHJ69_002186"
     CDS             21764..22369
                     /gene="ttdB"
                     /locus_tag="ACHJ69_002186"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO5015171.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLIGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            22417..23880
                     /gene="ttdT"
                     /locus_tag="ACHJ69_002187"
     CDS             22417..23880
                     /gene="ttdT"
                     /locus_tag="ACHJ69_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO5015172.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPLPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASNATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPILKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIICWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTIVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(23923..24936)
                     /gene="tsaD"
                     /locus_tag="ACHJ69_002188"
     CDS             complement(23923..24936)
                     /gene="tsaD"
                     /locus_tag="ACHJ69_002188"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO5015173.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            25174..25389
                     /gene="rpsU"
                     /locus_tag="ACHJ69_002189"
     CDS             25174..25389
                     /gene="rpsU"
                     /locus_tag="ACHJ69_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO5015174.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            25500..27245
                     /gene="dnaG"
                     /locus_tag="ACHJ69_002190"
     CDS             25500..27245
                     /gene="dnaG"
                     /locus_tag="ACHJ69_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO5015175.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADRNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            27440..29281
                     /gene="rpoD"
                     /locus_tag="ACHJ69_002191"
     CDS             27440..29281
                     /gene="rpoD"
                     /locus_tag="ACHJ69_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO5015176.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDTADDDNSIDPELAR
                     EKFAELRAQYIVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(29359..29865)
                     /gene="mug"
                     /locus_tag="ACHJ69_002192"
     CDS             complement(29359..29865)
                     /gene="mug"
                     /locus_tag="ACHJ69_002192"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO5015177.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QSLVVRGR"
     gene            29990..30065
                     /locus_tag="ACHJ69_002193"
     tRNA            29990..30065
                     /locus_tag="ACHJ69_002193"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30023..30025,aa:Ile,seq:cat)
     gene            complement(30119..30883)
                     /gene="nfeF"
                     /locus_tag="ACHJ69_002194"
                     /gene_synonym="yqjH"
     CDS             complement(30119..30883)
                     /gene="nfeF"
                     /locus_tag="ACHJ69_002194"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO5015178.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAKPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            31171..31794
                     /gene="nfeR"
                     /locus_tag="ACHJ69_002195"
                     /gene_synonym="yqjI"
     CDS             31171..31794
                     /gene="nfeR"
                     /locus_tag="ACHJ69_002195"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO5015179.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(31901..33421)
                     /gene="aer"
                     /locus_tag="ACHJ69_002196"
     CDS             complement(31901..33421)
                     /gene="aer"
                     /locus_tag="ACHJ69_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO5015180.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAVMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRVVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
BASE COUNT         8390 a         8940 c         8273 g         7949 t
ORIGIN      
        1 cagcatttaa tataaataac gagtcaaatt atctctggtt atggtaatgt ccatagctga
       61 attaatatta aattcaaacc tgtattttcc tcatccattt ataaaggatt atagtcatgt
      121 taaaaaaaca ttgttatcta tgttcgcaac cgcattgtta tcaggcgttg cttttaacgc
      181 tcttgctgac gatgctaatc agggttcagg taaaattact tttaaaggtg aagttatcga
      241 tgcactttgt tctattgctc ctggtgatga agatcagaca ataaacctcg gtgaagttgc
      301 tgataccgta ttaaaaagcg gtcagaaatc actgcctgta gatgtcacca ttcatttgca
      361 ggattgtatt ttatctgacg gcactaacac tgttgataaa gtcaaaatca cctttagttc
      421 tgccagtgtt gacgctaccg actccaacct gcttaaaaac actctggaag gtaacatcgg
      481 cggcgcaact gatgtaggcg tacgtctggt gaaatcagac aacaccaacg tgactcttgg
      541 cactccaatc actatcaact tcccgacgac taactcttac caggagttga actttaaagc
      601 ccgtatggag tctctgggac gcaccgcgac cccgggtaac gtgcaggcac aggctaatta
      661 cgtactcgac tacaagtaat cggtagtcaa tacctacaca acgccaggcg gtaaagccga
      721 tgaaatttca tcggcaactt tgggcctttt tagaaatgga tttttatgaa caataaaaac
      781 acgttttccc gggataagtt atcccatgca attaaaaatg ccctgtctgg cgttgtgtgt
      841 tccctactct tcgttttgcc agtccacgcc gtagaattca acgtcgatat gattgacgca
      901 gaagaccgtg agaatatcga catctctcgt tttgagaaaa aaggctatat cccccctggt
      961 agatacctcg ttcgtgtgca aataaataaa aatatgttgc cacaaacgtt aatactggaa
     1021 tgggtaaaag ccgataatga aagtggttcg ttactctgct taaccaaaga aaatttgact
     1081 aatttcggtc ttaatacgga atttattgaa tcattgcaaa acatagctgg cagcgaatgt
     1141 ctcgatttaa gccaacgtca ggagttaacg acacgacttg ataaagctac gatgatatta
     1201 tcgctaagtg ttccccaggc atggttaaaa taccaggcaa caaactggac gccaccagag
     1261 ttttgggata ccggtatcgc cgggtttatc cttgattaca acgtgtacgc cagccagtat
     1321 gccccacatc acggagacag cacccaaaac gtcagctcct atggtacgtt aggctttaac
     1381 ctcggcgcat ggcgcttacg tagcgattac caatataatc agaattttgc tgatggacgc
     1441 tcggtaaacc gcgacagcga atttgcgcga acttatctgt ttcgccctat cccctcctgg
     1501 tcgtcaaaat tcactatggg ccagtacgac ctgagctcca atctttacga taccttccac
     1561 tttactggcg catcgctgga aagtgatgaa agcatgctgc cgccagattt acagggttat
     1621 gcgccacaaa ttaccggcat cgcgcagacc aacgcgaaag taactgtggc acaaaatggt
     1681 cgtgtacttt atcaaaccac tgtcgcgcca ggccctttta ctatttctga tttggggcaa
     1741 tcgtttcagg ggcagctgga tgtcacagtg gaagaagaag atggccgcac cagcaccttc
     1801 caggttggct ccgcatccat tccctattta acccgtaaag ggcaagtgcg ctataaaacg
     1861 tcactgggaa aaccgacatc cgtcgggcat aacgatatca ataatccctt tttctggacg
     1921 gcggaagcct cctggggctg gctgaacaat gtgtcgttgt atggtggtgg catgttcacc
     1981 gctgatgatt atcaggctat cactaccggt attggcttta accttaacca attcggttcg
     2041 ctttcttttg atgtcaccgg agcagacgcg tctttacagc aacaaaatag cggcaatctg
     2101 cgtggttaca gctatcgctt caactatgca aagcatttcg aatcgacagg cagtcagatt
     2161 accttcgcgg gttatcgctt ctcagataaa gattacgtgt cgatgagtga gtacctcagc
     2221 tcgcgtaatg gcgatgagtc aatcgataat gaaaaagaga gttatgtcat ttccttgaac
     2281 cagtactttg aaacgctgga attaaactct tatctcaacg ttacacgcaa tacttattgg
     2341 gacagcgcca gcaataccaa ctactccgta tctgtaagca aaaactttga tattggcgat
     2401 ttcaaaggta tatctgcatc gctggcagta agtcgaatcc gctgggatga cgacgaagag
     2461 aatcaatatt acttctcttt ctctctacct ttacaacaaa accgcaacat ctcctacagt
     2521 atgcagcgaa cgggaagcag taatacttcg cagatgattt cctggtacga ttcatcagat
     2581 cgcaacaata tctggaatat ttcagcgtcg gcaacggacg acaatatacg tgatggcgaa
     2641 ccaacactgc gcggcagcta ccagcactat tcgccgtggg gacgtctgaa cattaatggc
     2701 agtgtacagc cgaatcagta caattctgtt accgcaggct ggtacggttc acttaccgct
     2761 acacgtcatg gtgtcgccct tcacgattat agctatggcg ataacgcccg catgatggtc
     2821 gataccgatg gcatctccgg cattgaaatc aactctaacc gtaccgttac caacgggctg
     2881 ggcatcgccg tgataccttc gttatcgaac tacaccacct ccatgttgcg ggtgaacaat
     2941 aacgatctgc cagaaggtgt cgatgtcgaa aactcggtta ttcgcaccac gctcacccag
     3001 ggtgccatcg gctacgcaaa actgaatgcc accaccggtt accaaatcgt cggcgttatt
     3061 cgtcaggaaa atgggcgctt ccctccacta ggtgtgaatg tcacggataa agcgacaggt
     3121 aaagatgtgg gcctggtcgc ggaagacggc ttcgtttatc tcagcggtat tcaggaaaat
     3181 agtactctgc atttaacctg gggtgataat acctgtgaag tcacgccgcc aaaccaaagt
     3241 aacattagcg aaagcgcaat aattttacct tgtaaaacag tcaaataatt caggatgaat
     3301 ataatgaaat caccattaat cacaacagga atgatttgtt tactggggat ctgcaacttt
     3361 gcccaggcga ctgtctcccc cgacagaaca cgcatcattt ttaatgcttc gaataaaagc
     3421 gcaacggttc gcctgaccaa ccaaagtaaa atcgatccct atctggctca gtcgtggatt
     3481 gaagatgcca gcggtaaaaa aacgcgtgat tacatctcga cattgccgcc gatggaacgt
     3541 attgagcctg atgaacagat acaaattcgc ctgatggcac tggcctcgtt aaacgactta
     3601 ccacaggacc gagaaacgtt gttctactat aacgtgcgag aaattccgcc gcgcgccaaa
     3661 gaacagaatg taatgcaaat cgccatgcaa agccgcctga agctattctg gcggccaaaa
     3721 gcgattgagt taaaagaggg ggagatgatt cctctgcaaa aagtaacgat tacccgcacc
     3781 gccgccggtc tgaccctgaa taacccgacg ccttatcata ttaccgtcgg ttatatcggc
     3841 actaatggca agacactgat gcccggtgcg gacagtatta tggtggcacc atttacttct
     3901 gcaactcaac acctaagcag cctgccctct accttccagt taggctttgt ggcggactac
     3961 ggcgggctgg agatgtttaa agtcgaatgt aattccatcc aatccctctg ccaaagctct
     4021 cctgctaaga aggggaaaat atgattacgc tttttcgttt actggcgatt ctttgcctgt
     4081 tttttaatgt ttcagctttt gctgttgatt gctatcagga tgggtacaga ggaacaaccc
     4141 tcataaatgg agatttacca gcgttcaaaa ttccagagaa cgcgcaacct gggcaaaaaa
     4201 tttgggagag tggagatatt aatatcacgg tttattgtga caatgcacca ggatggacaa
     4261 cagacaatcc atcagaaaat atctatgcct ggatcaaatt accccaaata aatagtgccg
     4321 acatgttgaa taatccatat ttaacctttg gcgtgactta taatggtgta gattatgaag
     4381 ggataaatga aagaattgat actcatgcat gcctggataa atatgagcaa tactataatg
     4441 ggtattatta tgaccctgta tgcaatggca gtacccttca aaaaaatgta acatttaatg
     4501 cccattttcg cgtctatgta aaattcaaaa gccgcccagc aggagatcag acggtgaact
     4561 ttggcatagt caacgtgtta caattcgatg gcgaaggagg ggcaaacatg tcctctaacg
     4621 cgaagaattt acgctatgtg attacgggat tagataatat ttcattcctt gactgtagtg
     4681 tcgacgtccg gatttttcca gaaagccaga tagttaattt cgggcaaatt gctgcaaact
     4741 ctattgctac ttatcgacca aaagctgcat ttagcgtatc tactataaaa gatgttgctg
     4801 ccgattgtac cgagcaattt gatgttgcca ccagctttta tacgacagat acgttacatg
     4861 atgatacgca tctggaaatg ggtaatggtt tgttgatgcg tattactgat caaaaaacac
     4921 aagaagacat taagtttaat cagtacaaac gcttcaccac atatattcca ggtcagactg
     4981 gagcgatggt tacccgtgat taccaggctg aactttcaca aaaaccgggg gagactctcg
     5041 tttatgggcc tttcaaaaaa gatctcatcg taaaaataaa ctataactga acagcaaaat
     5101 gccgggaaca ccccggcatt ttcatattta gtatttcacg tcattagctg gctttacgct
     5161 catgtgcctg acggtatgcc accaggtctt caatagtcac gagcgccata ttgtgtttat
     5221 tggcaaactc aatgcactct ggtgcacgcg ccatcgtgcc atcgtcatta gtcagctcac
     5281 acagcacacc agctggttta aagcctgcca gcgtcatcag atcgatagtc gcttcagtat
     5341 gaccgccacg cgtcagtaca ccacctgcct gcgcgcgaag tgggaaaacg tggcccggac
     5401 gattcagatc tgaaggtttt gcgccatcgg caatcgctgc gcgaacggtc gtaatacggt
     5461 cagcggcaga aacaccggta gtcacacctt cagctgcttc aatggtcacg gtaaaaccgg
     5521 tgccataggc gctggtgtta ttttctacca tcattggcag atcgagttgt ttacggcgat
     5581 cgtcagtaat gcacaggcaa acaataccac tcccgtggcg aatggtcagc gccatctgct
     5641 caacggtcat ggtttctgcc gggaagatca tatcgccttc gttttcacgg tcttcatcat
     5701 caagcaccat cacaccgcgt ccttcacgca gcgcagccag tgcattttca acacgttcga
     5761 aaggcgtacc aaaagaggaa agtagcgtct gattcatggt aaaaaaacct cactaaaatt
     5821 atggttacca gaatcagggc agtcttagga gtggcggcat atagccaaaa taacgtgagc
     5881 gggtccatgc ccgacagaat cgttactctc tcccatccgg actctaaccg tcggccccgg
     5941 aattacaccg gatctgctgt cctttgagtt cgcacccaaa gcgctcgcgg gctttcaact
     6001 gagttgattt accgccggtg gggaatttcg ccccgccctg agaataagcg gattcactat
     6061 aacgctaatg attagcgcca gcaacgcata gcttcacata attctggttt atgacttacc
     6121 cttatcgcac tacaatggca ctcaacacct atcagtacag ggaaactaca atgattgacc
     6181 cgaaaaaaat tgagcaaatc gctcgccagg ttcacgaatc aatgcctaaa ggaatcaggg
     6241 agttcgggga agatgtggag aaaaaaatcc gccaaaccct acaagcgcag ctgacgcgtc
     6301 tcgatctggt aagccgcgaa gaattcgacg ttcagacgca agtcctgtta cggactcgtg
     6361 aaaaactggc gttactggag cagcgcatca gcgaactgga agcgcgcaat aatccggtag
     6421 cagaacttca atctcctccg gctatcccgc cagttgataa agcagaataa agcaccacaa
     6481 aaacgggcca gacggcccgt aatatcagtg ttctaattca agttctctgg tttgcatcat
     6541 ctgttggcaa taccaggcat aacgtgcgca aaaccaggtt ctatgttctt catccatgtt
     6601 accagtaacg gccagaatat cgaccgggcg accttctgcg tgcattctgg cgaaactacg
     6661 cgcgagaaaa tcgaaattat ttaaagaatt aagactatga tccataagca catcctccgg
     6721 ttattgcata ccttttaagg ccatatgctt tttctgattt cagtattccc caatcagtgt
     6781 agtgccttta aacataacgt gcgtaaatat attgtccccg atcacacttt ttagtgaaaa
     6841 ataaaaaaac catattaaaa gactttctct tccagaacct ctctaacatg tgttattatt
     6901 aacaaagata aatgtaagca aggaaactat tccaaagtaa atattgcgtc actaaatgga
     6961 cattggagtg acatatgatt ttattcgccg actataacac cccttatctt ttcgctattt
     7021 cctttgtact cttgatcggc cttttagaaa tttttgcact tatctgcggt cacatgctct
     7081 ccggtgctct tgatgcacat cttgaccatt atgattccat aaccacaggt catattagcc
     7141 aggcacttca ttatctcaat attggaagat taccagctct cgtcgtcctc tgcttactcg
     7201 ctggtttttt tggtcttatt ggcatcctgt tgcaacatgc ctgcgttacg ctctggcaat
     7261 caccactccc caacctgttc gtggttcctg tcagtttact gtttacaatt attgcagtgc
     7321 attataccgg taaagtcgtt gccccctgga ttccacgcga ccatagttca gctattacag
     7381 aagaagaata tgttggtagc atggcattaa ttaccggcca tcaagccact tcaggtaacc
     7441 cttgcgaagg aaaactcacc gatcaatttg gccaaataca ttatttgcta ctggagcctg
     7501 aagaaggaaa aactttcact aaaggagaca aagtgttaat tatttgccga ctttctgcaa
     7561 cgcgatattt ggcggaaaat aatccctggc cccagatatt gtgatttatt aatcaaaagg
     7621 aaatttaata atggatgata tcgttaattc tgtgccctcc tggatgttta ccgcgattat
     7681 cgccgtatgc attctgttta ttattggaat tattttcgcc aggctctatc gtcgcgcttc
     7741 ggcagagcaa gcatttgttc gtactggttt aagtgggcaa aaagtggtga tgagcggtgg
     7801 cgcaatcgtg atgccgatct ttcatgaaat aattcccatc aatatgaata ccctgaagct
     7861 ggaagtcagc cgctcaacca ttgatagcct gattacgaaa gatcgtatgc gcgtcgatgt
     7921 cgtggttgct ttctttgtgc gggtaaaacc ttcagtagaa ggcatcgcca ctgcggccca
     7981 gacgctgggg caacgcactc tatcaccaga agatttacgt atgctggtcg aggataaatt
     8041 cgtcgatgcc ctacgcgcca ccgctgcgca aatgaccatg catgagttac aggatacccg
     8101 cgagaacttc gtgcagggag tacaaaatac cgtagctgaa gatctgtcga aaaatggcct
     8161 ggaactggaa agcgtttcac ttaccaactt taaccagacg tcgaaagaac atttcaatcc
     8221 taacaacgcc tttgacgccg aaggtttaac caagctgacg caggagacgg agcgccgtcg
     8281 tcgcgaacgt aacgaagttg aacaggatgt agaagttgcg gtgcgtgaga aaaaccgtga
     8341 tgcgctttcg cggaagctgg agattgaaca gcaagaagcg tttatgacgc ttgagcagga
     8401 gcagcaggtt aaaacccgta ccgctgagca gaatgcgaaa attgcggctt ttgaagctga
     8461 acgtcgtcgt gaagcagagc agacgcgaat tctggctgaa cgacagattc aggaaacgga
     8521 aatcgaccgc gaacaggccg tccgctcaag aaaggttgag gccgaacgtg aagttcgcat
     8581 taaagagatc gaacagcagc aggtcaccga aatcgctaac cagacgaaat cgatcgctat
     8641 tgccgccaaa tcggaacaac agtcccaggc agaagcgcgt gctaaccttg cactcgcaga
     8701 agcagtaagc gcccaacaaa acgtagaaac cactcgccag actgccgaag ccgatcgtgc
     8761 taaacaagtg gcgttgatcg ccgcagcgca ggatgcagaa accaaagcgg ttgaactgac
     8821 cgtgcgggcg aaagcagaaa aagaagccgc agaaatgcag gcggcggcta tcgttgagtt
     8881 agccgaagct acacgtaaaa agggtctggc ggaagcagaa gcacaacgtg cgctgaacga
     8941 tgctatcaac gtactttctg atgaacaaac cagccttaaa ttcaaactgg cattattgca
     9001 ggcgctgcct gcggtaattg agaaatccgt ggaaccaatg aagtctatcg acggcatcaa
     9061 aattattcag gtcgatgggc tgaatcgtgg tggcgctacg ggtgatgcaa acacgggtaa
     9121 tgtggggggc ggaaacctgg cagaacaagc gttatcagcc gcactctctt accgtacaca
     9181 ggcaccgctg attgactcct tgctcaatga aattggcgtt tcaggcggct cactggcggc
     9241 attgacttca tccttaagct caacaactcc cgtcgaagaa aaagcagagt aaaccctctt
     9301 ctatgaataa tccctggccc aaaaagccgg ggatttttta tatctgcgtt ccgctaaaag
     9361 gtgcaaatga tcaagctgtt gcagcatttt gcgtgactgc tcggggaagg caaaactgcc
     9421 tctgggaaag cgttgcgcgg ggtccggcgc tcatcaacaa tcggggagtc agcaaggggc
     9481 tgaaacggga aagcccctcc cgaggaaggg gccttgtata aggaaagggt tatgatgaag
     9541 ctcgtcatca tactgattgt gttgttactc gtaagtttcg cagcttatta acagccaatc
     9601 agagggggga gaaatcctcc ctcacccttg ttcctttact ttaggttgaa aaaaaatcag
     9661 cgtcaatagg cccgcctgct acaaagcgag atctgtgaac cgctttccgg ttagcctttt
     9721 ttatcctgtt ggatcttctt gatgatgttg gtcgtcgagc aaccgtcttc aaagttgagc
     9781 accagcactt cgccaccgtt ggcccagact tctttactcc cggcaatctc ttctggttta
     9841 tagtcgccgc ctttcaccag tagatctggc aagatcccgg cgatcaagcg ctgcggcgtg
     9901 tcctcttcaa acgacaccac ccagtcgacc gcttccagtg cgcccagcac aatcatacgc
     9961 tgttcgagcg ggttaacggg gcgggaatcc cctttcagcc gtttggtgga ggcatcgctg
    10021 ttcacagcga caatcaaacg gtcacccagt ttacgggcat ttgccagata agagacgtgc
    10081 ccagcgtgca ggatgtcaaa gacaccgttg gtcatcacca ctttttcacc gcgtttacgc
    10141 gctgccgcta cagcaagctt cagttcctct tcggtcatca caccaaagcc ggtatctgca
    10201 cgtccacgta cagcgttttc cagctcgatc ggcgaaaccg tggatgtccc cagtttgccg
    10261 accaccacac cagccgccgc attggcaaag aagcaggctt cttccagtga attacccgct
    10321 gccagcgttg ccgccaggac gccaatcacc gtgtcgcccg caccggtaac gtcatacact
    10381 tcctgcgcct gggttggcat atgcagtggc gctttacccg gttgcagcag cgacataccc
    10441 tgttcggaac gggtcactaa cagagccgag agttcgtaat cggcaatcag tttcatgccg
    10501 cgctcaacaa tctcttcttc ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga
    10561 ttcggcgtta acagcgtagc gccgcggtag cgctcaaaat cggtaccttt tggatcaatc
    10621 agcaccggaa cacccgcttt acgcgccagt tggatcatct gctgtacgct tgccagcgca
    10681 cctttggcgt agtcagaaag caccagcgcg ccaatcgaac tcagtgcctg attaatccgc
    10741 tcgtgcagcg gctgcggatc aaccccttcg aaaccttctt cgaaatccag acggatcagt
    10801 tgttggttgc gggaaagtac ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaaca
    10861 aagtcgcatt tgacgttgac gtcggccaga gatttactga gcgcgcgcgc cgcatcgtca
    10921 atgcccgtca acccgaccag gcgtgcatta gcaccgagag aagcgatatt catcgccacg
    10981 ttagccgcgc cgcccggacg ttcttcgatg gtattcactt taaccacggg caccggcgct
    11041 tccggcgaga tacggctggt gggaccgtac cagtaacgat ccagcatcac atcaccaacc
    11101 accatcactc ctgcacgttc aaactctggc agcgttactt tcattcctgt ctcctgagag
    11161 attcaaaatt tgcgcgcgat aataccatac ttcattcttc caccagccac ttctgccagc
    11221 ttgcccgcac cagttcacgc tctgcggtga agcaattctc cgacacatga cccggcaatt
    11281 cctgtaatgc cagatgatga agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca
    11341 tcgcttcctg ctcttccata atgtcgtttt gcgccagtag ttccagaatg cgcacgttgt
    11401 ctgaccagcg cgttaacttc ggtttttcgt gagcgtagcg caacaccaga tattgagtaa
    11461 taaattcgat atcggtaatt ccgccttcat cggctttgat atcaaagcga tcgcgatgtt
    11521 tattgccgag atgggcacgc attttctcgc gcatttcccg cacttctgtt tgcagggttt
    11581 tgccttcgcg cggcagcgtc ataatctccc ggcgtactgc gtcaaagtgc gcggtgagct
    11641 gcggatcgcc gtacactaca cgcgcacgca ccagcgcctg atgctcccac gtccaggcct
    11701 cgtttttctg ataatcagca aatgcttctg cggatgtcac cagcattccc gccgccccgg
    11761 acggacgcag tcgtgcatcc acttcataca aaatgccgga agaggttcgc gtactgaaca
    11821 gatgcataat gcgttgcgcc agacgcagat aaaactgtcg cccgtcgatt tcccgctcgc
    11881 cgtcagtcat cgcatccatt gggcaatcat ggaggaagat caggtcaaga tcggaactgt
    11941 agcctaactc ccagccaccc agcttgccgt agccgaccac cgcaaaacca cgcccttcgc
    12001 gttcgttcag gtgatttggc ttaccgtagc gggcaaccat ttgaacccac gcctgctgaa
    12061 cgacggcatc aatcatggct tctgccagcc aggttaagtg atcgctcact ttcattaccg
    12121 gtagcgtacc ggcgatatcc gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac
    12181 gcagcgcctc aagctgttgt tcttcgtcat cttccggcac gcgcagcaaa tactggcgca
    12241 gttcatcacg gtaggcatcc gtcgccgttg gttggtagag ggtatttgga tcgagcaatt
    12301 catccagcaa taatggataa cgcgctagct ggctggcaat catcggcgat gcagcacaca
    12361 gagaaatcaa atgtttaagc gccgcaggga attcactaag cagctcaaga taagtggtgc
    12421 gagtaacaat ccccaccagc aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac
    12481 gcgcacagac atcacttagc agatgtggca tcagatggtc gagcacctga cgtcctcgcg
    12541 gcccgatggt gcgcttatcc agctctttgc ggaaatcagc aatcagcatt agcacctgtt
    12601 tgcgatcatc ctcgctaaga tgcgccagca ctggcgtggt gtcatcttcc tgcaacgcat
    12661 cctgccacag ctcacgccac tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt
    12721 cgccaatcaa ttcattaaac acccggcgca cattggccat atgtgcggtc agcaccccgg
    12781 tcaattgcgg ccagtcagca aagtccatcg cccacgtcag ccgcgcacgg ttaagctcat
    12841 caaaaggaag cgtctgggtt tgttcgtcgt taatgctttg cagcaggttt tccagacgcc
    12901 gcaggaacag atacgccact cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg
    12961 cggcaatggc gctgagcgtt ggcagtaaag agcgcgattg cagcgacggt tcgcgcccgc
    13021 cgcgaatgag ctggaacacc tggacgataa attcaatttc gcgaatgccg cctgcgccaa
    13081 gtttgatgtt gtcggtcaaa ccgcgtcgac gcacttcacg ggcaatcatt cctttcatgt
    13141 tgcgcagcga ctgaatcacg ctgaaatcga tgtaacgacg gaaaacaaac ggacgcagca
    13201 tcgcacgcaa ctcgttagca tagacgccgt cgctatcccc cataatccgc gccttgacca
    13261 tcgcgtaacg ctcccagtcg cgcccctgct cctggtaata atcttccagc gccgcaaaac
    13321 tcagcaccag cgggccactt tcaccaaacg gacgcagccg catatccacg cgatagacga
    13381 aaccatcctg cgttggttga tccagcactt taatcagccg ctgccccatg cgggtaaaaa
    13441 actgcgcgtt atccagttcc cggcgtccgc cctgggtaca accatgttcc ggccaggcaa
    13501 aaatcagatc gatatctgag gagaaattca gttccccacc gcccagctta cccatgccta
    13561 aaatcagcag cggttgcgct tcgccctgcg cattgcacgg cgttccccat tcgcggcagc
    13621 aggcgtcata cagccagtca cgcgccgcaa caatcagcgt ctccgccaga tggctgagct
    13681 gctgcaatat gctctcttca gtaaccagtg ccagcgtttg cgcccaggcg atgcgcacca
    13741 taatgcgccg ccggaatagc cgcagctcgc gcattaaccc ggcttcgtca ctcacattac
    13801 tgagcgcctc ctgcaaccat gctgcgtaat gctgccattc gtcggcctgc ggcggttggc
    13861 tttccagttc cgtcagccac tctggatgcg caattatgct gtcctgcaga aaatcactga
    13921 aagtaagtac tgacttcgcc tgtgcgctaa gtgattcctc ggctaaaggc tctggcagcc
    13981 gctcaacaac ggtctgccag tactgctgta acggtgaaga gagcggcttc ataagcgatt
    14041 ttatccttgc ctgattaacg ttttccgctg tgcaaccaga acggttcctg attgtttgcc
    14101 tcattacgga aatgttcaat ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc
    14161 tgccagttct ccagccagtc ttgcgcgacg acaggatcgt agtaacccgc cagcaacagt
    14221 attaagtcaa tatcacgcgt caggcgtggc aattggtcac ggtagcgatc gcctaacggc
    14281 tggcagaaaa cgcttttcag ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt
    14341 ttgaaggaat cgctgatttt gctctgcgct ttggcatcta aaaacggctg ccaggctttg
    14401 cttaccagcc attcggtcaa cgccagcttc gccattgccg tttcggtaga gtaaaccgcc
    14461 gttacggcag aaaccgcaga agcaatggtc gcctcgcatt gagtcagcag atcacgtaag
    14521 tgagtgctcg ctttacgcgg cacgataccg ccgaacagca tcagtgtatg acggaccagg
    14581 ccaatggctg ccagcacctg ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca
    14641 tgatactgcc attgcgctaa cgccagctcg aacgccgctt ccagcccctg ttccacatca
    14701 gcttttgccg caacatgcaa aatggtggtc ggtttgattt cacgcgccgg attgccctgc
    14761 gccagatgat agccacgcgc cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat
    14821 accagttggt tcgccagttt cagcaccgcg cgcgtgtcgc cgctaagcag ttccagttcc
    14881 agctcgcaga taggttcagc aaattcaccc gctttcactt ccccctggtc gagggcgatt
    14941 tcaatttggc taccatcaac ctctaccagc cacttttcgc gataaaaatc ggtgctaaac
    15001 agcggctgca cgcgggaagc gagatcggta ggcaattcgc cgttcggcca gacttccgtc
    15061 ggtaactgcg ccaggtcgag cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg
    15121 tgtaagccgc ctgtcactct tcctgcaact ttcatggtca tctcatagcg accgttttcg
    15181 ccacgaatac gcaagcccat gtcgtgccca cgcagccagt tatccggcgt ttcgtagtaa
    15241 atattcagca actgcacggg atcatggtgc tcgccgccca gcgtattgag atggtcacgc
    15301 aacgcctcaa cggcactgtg attaacaata aactttaatt cgatttcctg agccatagcc
    15361 tttacttatc ggttatgtca cataagtgac gatgaacggc gaacttaatg cgatcttttt
    15421 tgtcagtaga tagtattttg cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa
    15481 agtttgaagc agtggcaaga cctgacacag aggattccga ttgatgtcga atgctttgcg
    15541 tagcgacact gatgccacta ctatcgttcc actttccatg acaataacga cagcctgatg
    15601 ccaaaattac gcctgatcgg attaacttta ctcgcactta gcgcgactgc cgtctcacac
    15661 gctgaagaaa cgcgctatgt ttctgacgaa ctgaatacct gggtccgtag cggtccggga
    15721 gatcattatc gcctcgtggg cacggttaac gccggcgagg aagtgacctt attacaaact
    15781 gacgccaaca ccaattatgc ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg
    15841 ttgaaacaac ttagcacgga gccaagcctg cgttcccgtg tgccagatct ggaaaatcag
    15901 gtcaaaacgc tgaccgataa actcaccaat atcgataaca cctggaatca gcgcaccgca
    15961 gaaatgcagc aaaaagtggc gcagagtgac agcgtgatca acgggttaaa agaagaaaat
    16021 cagaaactga aaaacgagct gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag
    16081 ctggatgaca aacagcgcac catcatcatg cagtggttta tgtatggcgg tggcgtgctg
    16141 gggcttggct tgctgctcgg tctggtactg ccgcacctga tcccaagccg caaacgcaaa
    16201 gatcgctgga tgaactaaat cgccttcttc gttgcactgt cttattatta ggaattgtgt
    16261 atttgacgaa gggatgatgg cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca
    16321 ttgttagggc taccggtcaa agacagagat tgggtggtgg ttggcagtac gccacaggag
    16381 atgctcgacg cgggctacca gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa
    16441 acgcatgaag agtatgcact ggcacgtacc gaacggaaat ccggttccgg ttacaccggt
    16501 tttacctgct atgccgcacc ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg
    16561 accattaatg cgctggccca ggacgataac ggcgagatta tcgacccgta caacggtctg
    16621 ggcgatctgc aaaatcgtct gttgcgccat gtttcccccg cttttggcga agatccgtta
    16681 cgcgtgttgc gcgtagcgcg ttttgctgcg cgttatgccc acctctgttt ccgtattgcc
    16741 gatgaaactc tggcgctgat gcgcgagatg acccatgcgg gtgaactgga acacctgacg
    16801 cctgaacggg tatggaaaga gacggaaagc gccctcacca cccgcaatcc ccaggtgttc
    16861 ttccaggtac tgcgagattg cggcgcactg cgcgttttat tcccggaaat tgacgccctg
    16921 tttggcgttc cggcccctgc caggtggcac ccggaaatcg acacgggtat tcatacctta
    16981 atgacgctct caatggcggc gatgctgagt ccgcaggtcg atgtccgttt cgcaacgtta
    17041 tgccacgatc tcggtaaagg actgacgccg ccagaacttt ggccacgtca tcatggtcat
    17101 ggcccggcgg gtgtgaagtt agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa
    17161 attcgcgatt tagccagact ggtagctgag tttcacgatc tcatccacac ctttccaatg
    17221 ctgaacccga aaaccattgt taaattgttt gattccattg acgcctggcg taaaccgcag
    17281 cgtgtcgagc aactggcgct gaccagcgag gctgacgtgc gcggcagaac cggttttgaa
    17341 tcagcggact acccgcaagg ccgctggttg cgcgaagcct gggaagtggc gcagtcagtg
    17401 ccgacaaaag ccgtcgttga agcaggattt aaaggtgtgg agattcgtga ggagctgacc
    17461 cgacggcgga ttgcggcggt agccaactgg aaggaacaac gctgcccaaa gtctgagtaa
    17521 ttgttggata ttttgccgga tgcggcgtaa acgccttatc cggctacata tcaacgacgg
    17581 ttgtaggcct gataagacgc gcaagcgtcg catcaggcgt tgattgtcgg atgcggcgtg
    17641 aacgccttat ccggcctacg caatcgagga caattccagg catccgacac tattcttcac
    17701 atcaaaagaa cacgacgtac accgcagccg ccacaataaa gcgataaatg gcgaatggga
    17761 taaacgaaat gcgcttaatc aattgcagga aggttttaat cgctatcagc gccaccacaa
    17821 aagcggtgat aaacccaacg gcaaacatcg ggatatcgcc ggtagtcagg aagccccagc
    17881 ttttgtagag atcgagcgcc gttgcgccca tcatcatcgg caccgccagc aggaacgaga
    17941 actcggaagc agcgtaacgg ctcaccccca tcagcatccc acctgaaatg gttgccccgg
    18001 aacgggagaa acccggccac agcgccagac actggaaaca gccaatcata aacgcctgac
    18061 gataggtcat atcatcaaga cccggcgcac gcggctcttt cggcttcagg cattcggcgg
    18121 caatcagcaa cagaccgcca acgaccagtg catacatgac atttatcggg ttaaacaatg
    18181 atttaatcgt gtcgtggaac aacagcccca gtaccaccgc cggaatcatc cccagcaaga
    18241 tgtggatcag cgttaaacga cctttgcttt ctccttcgtg ctgcaacggg cgaccaaagt
    18301 ggatgccaat caggccaaac agacgccgcc agaacatcac tactaccgcc agaattgatc
    18361 ctaactggat cacgacttca aaggttttcg ccgtgtcgcc ctcaaacccc aacaagtgac
    18421 cgacaataat catatggccc gtgctggata ccggcagaaa ttctgtcaat ccttcgacca
    18481 cacccaatat tgccgctatc agcagcgagt gcatatcgct catcaataaa cccctaaatt
    18541 ataaaaatgt aacgcttgtc cgaactactg cagtatgacc aggttataat cgtttggttt
    18601 aacagctgta aaattaatta ttttctttca gattattgcc acgctcaatg attacgccaa
    18661 cattcgccgc ccgcgccact gcgcctggct tgctgagttt gatacgcacc cacggcgagt
    18721 tgaagcgtgc taacagcagc tccgccacct cttcagccac gcgttccacc agcgcaaaac
    18781 gcgccccctc gacgtggctg accaccgttt ctgcaatgtc agcgtaactg aggcaatccg
    18841 ccacatcatc acttttcgcc gctttacggt tatcccacgc catttcgata tcgaacacta
    18901 acttctgttc gatggtctgt tcccagtcgt aaacaccaat agtggtgatt accgaaagtt
    18961 gctctataaa tacaatatcc atcacgtcct gcctgctttt tggctaaccg gataccactt
    19021 ccggcgaaat gtgcgtatta tccacagatt catcgttgaa cacgaatttt caaaacggaa
    19081 cagcttatga gtgcaatcgc gcctggaatg atcctcatcg cgtacctctg cggctccatt
    19141 tccagtgcca ttctggtttg ccgcttgtgt gggttgcccg atccgcgaac cagcggctcc
    19201 ggcaatccag gcgcaaccaa tgtgttacgt atcggtggca agggagcagc cgtagcagta
    19261 ctgattttcg acgttctgaa aggaatgttg cccgtctggg gcgcgtatga attaggtgtc
    19321 agccccttct ggctaggctt aattgccatc gccgcctgtc ttggacacat ctggcccgtt
    19381 ttcttcggtt ttaaaggagg aaaaggcgtt gctaccgctt ttggtgccat cgcaccaatt
    19441 ggctgggatc tcactggagt aatggcggga acctggttac tgaccgtgct attgagtggt
    19501 tactcgtcgc tgggagcgat tgtcagtgca ctgattgctc cgttttatgt ctggtggttt
    19561 aagccacaat tcaccttccc ggtttcgatg ctctcttgcc tgatcctgct gcgtcatcat
    19621 gacaacatcc agcgtctgtg gcgtcgtcag gagacaaaaa tctggacgaa attcaaaaga
    19681 aagcgcgaaa aggatcccga gtgatttctg gtggatctac atgatctgat agccttcgtc
    19741 gggcttgccc agccgttgct ggcaccatgc cgccagaaat tccacacaga cacgtaattt
    19801 catgctgcga tagagcggct cccggtaaac agcccagata ttggcgcttt gtgcatactc
    19861 cggcaatact cgcaccagtt tgccactctc cagaaacggc aacacatccc actcggaacg
    19921 cagcataatc cctttgccct ccagcgccca ttgcagcaca atctcgccgc tattggagga
    19981 aagatgtccg cttaccttca ccgatttttt ctcctgaccg ttccccaact cccatattcc
    20041 gtgggtcata tcgcgctctt tggtcaccag gcagtcatga cgacttaatt cctgtaagga
    20101 ttgtggctgc ggatattttt gcagatactc aggtgctgcg cataatattc ttttattttt
    20161 tgtcaaaaga tgcgcaatat aataatcagg aattgcgtca ttaatacgaa tatccagatc
    20221 aatattatcc tgcactaaat caatttgtcg atcgaacaat tcaaaatgca cctgtaactc
    20281 aggataattg cgcatcagtt cggtaatagc tggcgcaata tggctgcgcc caaaaccaaa
    20341 actgcaacca atgcggatca tcccctccgg gcgcgttttg atttgcgtga cgtcatcgac
    20401 cagtcgctga tattgggtga ggatttccag cgcatgttcg taacaacgct ggccactttc
    20461 agtcagcgcc acgccgcgag cggagcggtt cagcagagtt gttgccagcg tattttcaag
    20521 gatttggatc cgcttggtga caaatgcagg cgtttgccca agcgtcgccg ctgcagcact
    20581 aaaactcccg ctatggacta tttccaccag tacctgcaag tctttggcta aaggccagct
    20641 atttagcatt gttaaatatc cgcagttttg ataaacgcag tgtatttcgc aaaacattac
    20701 cattccctgc tctggcgcaa tattcgttct tattttcgcg ttgattcacg aactgttaat
    20761 tacatattca cggtggcaaa aaatataaaa ccacattttt agtggtagtt tgtggcggtg
    20821 aatttttcca gacaaataca taaactggag ttgccatgat gagcgaaagt aataagcaac
    20881 aggcagtgaa taagttgacg gagattgtcg ctaactttac cgccatgatt tctacccgaa
    20941 tgcctgatga cgtggtggat aaactaaaac agctaaagga tgccgaaaca tcgtcgatgg
    21001 gaaaaatcat ctaccacacg atgttcgata acatgcaaaa agcgatcgac ctgaatcgtc
    21061 ctgcctgtca ggacaccggc gaaatcatgt tttttgttaa ggtcggttcc cgtttcccac
    21121 tgcttggcga gctgcaaagc atactcaaac aagccgtgga agaggcaacc gtcaaagcgc
    21181 cactgcgtca caatgcggta gaaatttttg acgaagtaaa caccggcaaa aataccggca
    21241 gcggcgtacc gtgggtcacc tgggatatcg tccccgacgg tgacgatgcg gaaatcgaag
    21301 tttacatggc aggcggcggc tgcacgctac ctggccgatc gaaagtgtta atgccgtcag
    21361 aaggctacga aggcgtagtg aaattcgtct tcgaaaatat ctccaccctc gccgtaaacg
    21421 cctgtccgcc ggtacttgtg ggcgttggca tcgctacctc ggtggaaacc gccgcagtgc
    21481 tctcgcgaaa agccattttg cgcccgattg gcagccgcca ccccaatccg aaagcggcag
    21541 agctggagct acgcctggaa gaaggactca accgtttggg gattggtcca caagggctga
    21601 ctggcaacag ttcagtgatg ggcgtgcata tcgaatctgc cgctcgccat ccgtcaacca
    21661 tcggtgttgc tgtttctacc ggctgctggg cgcatcgtcg cggcacactg cgagttcatg
    21721 ccgatctcac ctttgaaaat ctgtctcata cccggagcgc gttatgaaaa agatcctgac
    21781 aaccccgatc aaagctgaag atctgcaaga tattcgcgtc ggcgatgtga tctacctgat
    21841 cggtacgctg gtgacctgcc gtgacgtttg tcaccgccga ttgatcgaac tgaaacgtcc
    21901 gatcccttac gatctcaacg gcaaagcgat tttccacgct ggccccatcg tgcgcaaaaa
    21961 cggcgacaaa tgggagatgg tgtccgtcgg cccgacaacc agtatgcgta tggaaagttt
    22021 tgaacgtgaa tttatcgagc agaccggcgt gaaactggtg gtcggcaaag gcggaatggg
    22081 gccgctgacc gaagaaggct gccagaaatt caaggcgctg catgtgattt tcccggctgg
    22141 ctgtgcggta ctggcggcaa ctcaagtgga agagattgaa gaggtacact ggacagaact
    22201 cgggatgcca gagtcgctgt gggtctgccg ggtcaaagag tttggcccgc tgattgtctc
    22261 tatagatacc cacggcaaca acctgatagc cgaaaacaaa aagctgttcg ccgaacgccg
    22321 cgatcccatc gtggaagaga tttgcgagca cgtccattac atcaaataac cctcacggag
    22381 aggctcgccc ctctcctttt tctcaggcat aacacgatga aaccttccac tgaatggtgg
    22441 cgataccttg cgccgctggc ggtcatcgcc attattgctc tacttccgct tcccgcaggg
    22501 ctggagagtc atacctggct ctactttgcc gtttttactg gcgtgatcgt tggactgatc
    22561 ctcgaacccg tgccgggtgc cgtggtggcg atggtaggta tatccatcat cgccattctt
    22621 tcgccgtggc tgctgtttag cccggagcag ctcgcccagc caggttttaa gttcaccgcc
    22681 aaatccctct cctgggcggt ttctggtttt tctaattcag ttatctggct tattttcgcc
    22741 gcctttatgt ttggtacggg ctatgaaaag accgggcttg gacgtcgcat cgcgctgatt
    22801 ctggtgaaaa aaatggggca tcgcacgctg tttctcggct atgcagtgat gttctccgaa
    22861 ctgatactcg cgcctgtaac accgtccaac tcggcgcgtg gtgcggggat tatctatccc
    22921 atcatccgta acctgccacc gctctatcaa tcacaaccaa acgacagcag ttcgcgcagc
    22981 attggctcgt acatcatgtg gatggggatt gttgccgatt gcgtgaccag cgccattttc
    23041 ctgacggcga tggctccaaa tttgctgtta attggactga tgaaaagcgc atctaacgcc
    23101 acgctgagct ggggcgactg gttcctcggg atgttgccgc tcagtatttt actggttctg
    23161 ctggttccct ggctggctta cgtgctgtac ccgccgatac tgaagtctgg cgatcaggtg
    23221 ccgcgctggg cagagacgga actgcaggca atgggcccgc tctgttcgcg tgaaaaacgg
    23281 atgctggggc tgatggtagg cgcactggtg ctgtggattt ttggcggtga ttatatcgat
    23341 gccgcgatgg tcggttacag cgtggtggcg ctgatgctgc ttctgcgcat tatctgctgg
    23401 gacgacattg tcagtaataa agcggcgtgg aacgttttct tctggctggc ctcgcttatc
    23461 accctcgcta ccggactcaa caacaccggt tttattagct ggtttggcaa actgttagca
    23521 ggcagcttaa gcggttattc gccaacgata gtgatggtag cgttgattgt ggtcttttat
    23581 ctgctgcgct acttttttgc cagcgccaca gcgtatacct ccgctctcgc accgatgatg
    23641 attgccgccg cgctggcgat gccggaaatc ccgctgccgg tgttctgcct gatggttggt
    23701 gcggcaattg gtctggggag cattcttaca ccatacgcca ccggacccag tccgatttac
    23761 tacggcagtg gttatctgcc aacggcggat tactggcgac tgggggcgat ttttgggctg
    23821 atattcctcg tattgctggt gattaccggc ttactgtgga tgcccgtggt gttgctttaa
    23881 gtgaggacgg tgccggaaat tgctctccgg cacccaatag ttttacgcag ccggtaactc
    23941 cgccagcggc cagcgcggac gcacactcac gccgagatcc gccgtcgcgc ctgctttaaa
    24001 ccgcaccatt ccggcatagg cgatcatcgc accgttatca gtgcaaaatt ccggacgcgc
    24061 gtagaacact tcgccacgac gctttttcat catttcagcc agcttcgccc gcagtgtgcg
    24121 gttagcactc acgccgcctg ccatgaccag tcgcttaaag cccgtctgat ccagtgctcg
    24181 cttacactta atcatcaacg tatcgaccac tgcatcttca aaggcgcggg cgatatcggc
    24241 acgcgtctga tcgtcagtgc cgttatcacg aatggtattt gccgcgaagg ttttcagacc
    24301 agaaaagctg aaatccagcc ccggacggtc ggtcatcggg cgcgggaaga caaagcgccc
    24361 ggcagtaccc tgagccgcca ttttcgacag taatggcccg ccaggataat ccagccccag
    24421 cagcttcgcg gttttatcaa acgcttcacc agccgcatca tcgatagact cgccgagcag
    24481 ctcgtactga ccaatgccag tcacgctgat taactgcgta tgaccgccgg aaaccagcag
    24541 cgcgacaaac ggaaattccg gtgggttatc ttccagcatc ggcgctaaca gatgcccttc
    24601 catatggtgt accgggatcg ccggaacgtc ccaggcaaac gccagagaac gccccacggt
    24661 cgcgccaacc agcagcgcgc cgactaatcc agggcctgcg gtataggcca cagcatcaat
    24721 gtcttttgcc gttaaacctg actcctttag cgccgcctgg atcaacggta cggttttacg
    24781 cacgtgatcg cgggaggcca gttcaggcac gacgccgccg tagtcagcgt gcaatttcac
    24841 ctgactatac aattggttgg ctaacaaacc tttttcatcg tcgtaaatgg cgatgccggt
    24901 ttcatcgcag gaagtttcaa tacccagtac acgcatgact tgttttacct cgctttatta
    24961 ccgcgcagtg taggaccaat gcgggttgat gtaaaacttt gttcgcccct agagaaagcc
    25021 tcgtgtatac tcctcaccct tataaaagtc cctttcaaaa aaggccgcgg tgctttacaa
    25081 agcagcagca attgcagtaa aattccgcac cattttgaaa taagctggcg ttgatgccag
    25141 cggcaaaccg aattaatcaa aggtgagagg cacatgccgg taattaaagt acgtgaaaac
    25201 gagccgttcg acgtagctct gcgtcgcttc aagcgttcct gcgaaaaagc aggtgttctg
    25261 gcggaagttc gtcgtcgtga gttctatgaa aaaccgacta ccgaacgtaa gcgcgctaaa
    25321 gcttctgcag tgaaacgtca cgcgaagaaa ctggctcgcg aaaacgcacg ccgcactcgt
    25381 ctgtactaat tccccgagag cgttgctctc cgatcagacc gagttgtagt tgtaaggccg
    25441 tgcttccgaa aggaatgcgc ggcttatttt cgtttatgaa ttgctaaaaa tcggggccta
    25501 tggctggacg aatcccacgc gtattcatta atgatctgct ggcacgcact gacatcgtcg
    25561 atctgatcga tgcccgtgtg aagctgaaaa agcagggcaa gaatttccac gcgtgttgtc
    25621 cattccacaa cgagaaaacc ccatcattca ccgttaacgg tgagaaacag ttttaccact
    25681 gctttggatg tggcgcgcac ggcaacgcga tcgacttctt gatgaactac gacaagctcg
    25741 agttcgtcga aacggtcgaa gagctggcag caatgcacaa tcttgaagtg ccatttgaag
    25801 caggtagcgg ccccagccag atcgagcgcc atcaacggca aacgctttat cagttgatgg
    25861 acggtctgaa tacgttttac caacaatctt tacagcaacc tgttgccacg tctgcgcgcc
    25921 agtatctgga aaaacgcgga ttaagccacg aggttatcgc ccgctttgcg attggttttg
    25981 cgccccccgg ctgggacaac gtcctgaagc ggtttggcgg caatccagaa aatcgccagt
    26041 cattgattga tgcgggcatg ttggtcacta acgatcaggg gcgcagttac gaccgtttcc
    26101 gcgagcgggt gatgttcccc attcgcgata aacgcggtcg ggtgattggt tttggcgggc
    26161 gtgtgctggg caacgataca cccaaatacc tgaactcgcc ggaaacggac attttccata
    26221 aaggccgcca gctttacggt ctttatgaag cgcagcagga taacgctgaa cccaatcgtc
    26281 tgcttgtggt cgaaggctat atggacgtgg tggcgctggc gcaatacggc attaattacg
    26341 ccgttgcgtc gttaggtacg tcaaccaccg ccgatcacat acaactgttg ttccgcgcga
    26401 ccaacaatgt catttgctgt tatgacggcg accgtgcagg ccgcgatgcc gcctggcgag
    26461 cgctggaaac ggcgctgcct tacatgacag acggtcgtca gctacgcttt atgtttttgc
    26521 ctgatggcga agaccctgac acgctggtac gaaaagaagg taaagaagcg tttgaagcgc
    26581 ggatggagca ggcgatgcca ctctccgcat ttctgtttaa cagtctgatg ccgcaagttg
    26641 atctgagtac ccctgacggg cgcgcacgtt tgagtacgct ggcactgcca ctgatatcgc
    26701 aagtgccggg cgaaacgctg cgaatatatc ttcgtcagga attaggcaac aaattaggca
    26761 tacttgatga cagccagctt gaacgattaa tgccaaaagc ggcagagagc ggcgtttctc
    26821 gccctgttcc gcagctaaaa cgcacgacca tgcgtatact tatagggttg ctggtgcaaa
    26881 atccagaatt agcgacgttg gtcccgccgc ttgagaatct ggatgaaaat aagctccctg
    26941 ggcttggctt attcagagaa ctggtcaaca cttgtctctc ccagccaggt ctgaccaccg
    27001 ggcaactttt agagcactat cgtggtacaa ataatgctgc cacccttgaa aaactgtcga
    27061 tgtgggacga tatagcagat aggaatattg ctgagcaaac cttcaccgac tcactcaacc
    27121 atatgtttga ttcgctgctt gaactgcgcc aggaagagtt aatcgctcgt gagcgcacgc
    27181 atggtttaag caacgaagaa cgcctggagc tctggacatt aaaccaggag ctggcgaaaa
    27241 agtgatttaa cggcttaagt gccgaagagc gatcgggaag cccccgacag ccgcactgag
    27301 aggcagcggc aaatatataa gtacgccctc gtaattatcg ttggcggtaa acaaccgttg
    27361 gatttcagcg ttaacagctg aaggacatcg ggtcaatcgc ccaacaccaa cctcatgaaa
    27421 taagtgtgga taccgtctta tggagcaaaa cccgcagtca cagctgaaac ttcttgtcac
    27481 ccgtggtaag gagcaaggct atctgaccta tgccgaggtc aatgaccatc tgccggaaga
    27541 tatcgtcgat tcagatcaga tcgaagacat catccaaatg atcaacgaca tgggcattca
    27601 ggtgatggaa gaagcaccgg atgccgatga tctgatgctg gctgaaaaca ccgcggacga
    27661 agatgctgcc gaagccgccg cgcaggtgct ttccagcgtg gaatctgaaa tcgggcgcac
    27721 gactgacccg gtacgcatgt acatgcgtga aatgggcacc gttgaactgt tgacccgcga
    27781 aggcgaaatt gacatcgcta agcgtattga agacgggatc aaccaggttc aatgctccgt
    27841 tgctgaatat ccggaagcga tcacctatct gctggaacag tacgatcgtg ttgaagcaga
    27901 agaagcgcgt ctgtccgatc tgatcaccgg ctttgttgac ccgaacgcag aagaagatct
    27961 ggcacctacc gccactcacg tcggttctga gctttcccag gaagatctgg acgatgacga
    28021 agatgaagac gaagaagatg gtgacgacga taccgccgat gatgacaaca gcatcgaccc
    28081 ggaactggct cgcgaaaaat ttgcggaact gcgcgctcag tacattgtaa cgcgtgacac
    28141 catcaaagcg aaaggccgca gtcacgctgc cgctcaggaa gagatcctga aactgtctga
    28201 agtattcaaa cagttccgcc tggtgccgaa gcagtttgac tacctggtca acagcatgcg
    28261 cgtcatgatg gaccgcgttc gtacgcaaga acgtctgatc atgaagctct gcgttgagca
    28321 gtgcaaaatg ccgaagaaaa acttcattac cctgtttacc ggcaacgaaa ccagcgatac
    28381 ctggttcaac gcggcaattg cgatgaacaa gccgtggtcg gaaaaactgc acgatgtctc
    28441 tgaagaagtg catcgcgccc tgcagaaact gcagcagatt gaagaagaaa ccggcctgac
    28501 catcgagcag gttaaagata tcaaccgtcg tatgtccatc ggtgaagcga aagcccgccg
    28561 tgcgaagaaa gagatggttg aagcgaactt gcgtctggtt atttctatcg ctaagaaata
    28621 caccaaccgt ggcttgcagt tccttgacct gattcaggaa ggtaacatcg gtctgatgaa
    28681 agcggttgat aaattcgaat accgccgtgg ttacaagttc tccacctacg caacctggtg
    28741 gatccgtcag gcgatcaccc gctctatcgc ggatcaggcg cgcaccatcc gtattccggt
    28801 gcatatgatt gagactatca acaagctcaa ccgtatttct cgccagatgc tgcaagagat
    28861 gggccgtgaa ccaacgccgg aagaactggc tgaacgtatg ctgatgccgg aagacaagat
    28921 ccgtaaagtg ctgaagatcg ccaaagagcc aatctccatg gaaacgccga tcggtgatga
    28981 tgaagattcg catctggggg atttcatcga ggataccacc ctcgagctgc cgctggattc
    29041 tgcgaccacc gaaagcctgc gtgcggcaac gcacgacgtg ctggctggcc tgaccgcgcg
    29101 tgaagcgaaa gttctgcgta tgcgtttcgg tatcgatatg aacaccgacc acacgctgga
    29161 agaagtgggt aaacagttcg acgttacccg cgaacgtatc cgtcagatcg aagcgaaggc
    29221 gctgcgcaaa ctgcgtcacc cgagccgttc tgaagtgctg cgtagcttcc tggacgatta
    29281 atcggtaggc cggatcaggt gttacgccgc acccggcact agtccctccg cacaaacgcc
    29341 accttttcgg tggcgttttt atcgcccacg caccaccagc gactggtcca gctcgcgata
    29401 cgcttcaacc agtttctcca gtgaaacgcg acttaaaccg ctgggatttg gcagcaccca
    29461 aatctgcgtc gaaccaatgg tgagagtttg tttcccccac tgtgcaccgc gctggctgaa
    29521 tccctgttca tatgcttgtt tgcccagaat cgccaacgcc tgcggctgat aatcttctat
    29581 tttttcaatt agcttacgcc cgcctgcgtg cagttcctgc tttgacactt cattggcttg
    29641 caccgttgga cggtctacca gtttggtgac gccacaacga taatccagca gatgctgtgc
    29701 ctcctgcggc ttcaactgac ggtcggtaaa cccggcctga tatatcacct tccagaagcg
    29761 gtttgccgga tgagcaaagg gaaaacccgt tccggcggat gaaagaccag ggttgatacc
    29821 gcaaaacacg acccgtaacc ctggagccaa aatatcctca accatattta ctcctttcta
    29881 acatcaccag gaaaagtata acggattgaa aatacattgt ttataaaaac agcaggcgcg
    29941 cggtaatggc tggattgcga cacggagtta ctttataatc cgctaccatg gccccttagc
    30001 tcagtggtta gagcaggcga ctcataatcg cttggtcgct ggttcaagtc cagcaggggc
    30061 caccagacat agcaaaggct gacgagaaat cgtcagcctt tttcttttta tatatcagtt
    30121 actttgcgtg ccagtaagcc gccgcgcgta cccgctgtgg gtcatactgt tccgcttcaa
    30181 agcgacggct taaattctta acgactttgc cttcgccggt tatccagatg aagtaatcat
    30241 cggcagggat ttgcatctgc gccagacgcg catctaccgc ctgctcatca tgtgccagcc
    30301 attcgatatt aaaaccatca aggtgcgcga gataatcctg acaggcgtta tcccgcacgc
    30361 taaccagcgc actaacttgc ggtttaacgg caagtttgct caacgtttcc aggcggcggc
    30421 gcagtgcggg cattccggat tcatcgcaga catacagctg atacgcgtaa tcctctggca
    30481 ccaccagcga accgcgcgga cctgccaccg taagtttatc gcccggtttc gcctgcatcg
    30541 cccagccgct ggcgacccca ccgtcgtgaa taaagaaatc gatcgccagt tcatggcgta
    30601 gttcgtcata cagcggcgta tagtcacgcg acggtgggcg tggtccttcc ggccagacga
    30661 tgccctcttc cgttaccgtt ggcggcacaa agtgagcgtc aggttgagga aagaagagtt
    30721 tgctgtgatc gtcaaagccg cgcgacgtaa aaccgtccag cgcctcgccg ccaaggacaa
    30781 tgcgctgaaa accggcgctg atgcgctcaa cgcgtagcac agtcagttca cggaagcgca
    30841 gatcattgcg aacgcgctgt gggtagcggg gggtgttatt cattgttatc gccttcgtga
    30901 tggtaatcag atatatctaa ataaattcca caaatgataa tgattgttaa tcatgataaa
    30961 tgcaagtgat ttgtagcact gatatatctg tagtctgata agactcgcaa cctcttatca
    31021 agcatcattg ctcaatgctg gatgcggcgt aaacggctta tccggcctac atgttgagta
    31081 aacctgcgtt taaaaatcat ttttgtactt gcagtaatgc ttatgtcaga tataaattag
    31141 atatatctaa ttaagcaaag ggaggctgat atgagccatc atcacgaagg gtgctgtaaa
    31201 catgaaggcc agccacgcca tgagggctgc tgcaaaggtg agaagtcaga acacgagcac
    31261 tgcggacacg gtcaccagca tgaacacggt caatgctgcg gtggtcgcca cggtcgcggc
    31321 ggcggtcgtc gacaacgttt ctttggtcac ggtgaattgc gtctggtgat tctggatatt
    31381 ctctcgcgcg atgacagcca cggttacgag ttgattaaag cgattgaaaa tctgacccag
    31441 gggaattaca ccccaagccc gggcgtcatc tacccgacgc tggattttct gcaggagcag
    31501 tcgctgatta ccattcgcga agaggaaggt ggtaagaagc agattgcgct gaccgaacaa
    31561 ggcgcgcagt ggctggaaga aaaccgcgaa caggtagaga tgattgaaga acgcatcaaa
    31621 gcgcgttgcg ttggcgctgc gctgcgccag aacccgcaaa tgaagcgggc gctggataat
    31681 tttaaagcgg tgctggattt acgcgtcaac cagagcgata tcactgatgc acaaataaaa
    31741 aagatcattg cggtgatcga ccgcgccgct tttgatatta cccaactgga ttaatcaccg
    31801 attgccggat gcagcgtacc ctacacaccc cgtaggcctg ataagatgcg ccagcatcgc
    31861 atcaggcatt gtgctccaac cgccggatcc ggcataccga ttaatgcagc accgtcaccg
    31921 cgtcttccag tcggctggcg cggtgtttca ccatcgccga cacctgcgca ctctcttcca
    31981 ctaactcggc gtttttctgg gtgatgagat taagctcatc cactacacgg gtcaggctgg
    32041 aaagcccatc ggcctgttcc agcgttgaat ggctaatctg agctatcaac tgggtgacgt
    32101 tttttacctg cgccacaata tcttccatcg tccgtccggc ggcgtgtacc tgctgcgaac
    32161 cagattgcac cttatcagca ctggcatcaa tcagcttgcg aatatcgttg gcagcattgg
    32221 cgctgcggct ggctaaatga cgcacttccc ccgccaccac tgcaaaacct ttgccctgtt
    32281 cgccggcacg cgccgcttcc accgccgcat tcagggccag aatattggtc tgaaacgcaa
    32341 tatcgttaat cagcgaagta atggtgccaa tgcgctgggt actgtcggca atatcgtcca
    32401 ttgtcttgat caccgtagtc atcgcctccc cgccctgcac tgccgcatta ctggcggtga
    32461 tagaaagttt atcggcagcc gacgccgtgg cagagttctg tttcaccgac gccgccattt
    32521 ggttcatggt cgccaccgtt tgctgaacgt tatcaactgt ctgctgggta tgttcgttca
    32581 gttcatcggt gcctttcgcc agcgtctcac tgccgtttct gacactggac acctggcttg
    32641 agacatcatt aattagccaa cggcacatca ggccaagctg ccctaccgcg cgtaatgtca
    32701 gccccagctc atcgctgcga ttcagatgct caacactatt acgctctccg gtcgccacct
    32761 tcagtgcctg acgggcgaca ttttctatcg ggcggacaat ctgccattca aaacaggcgc
    32821 ttgccaacaa taccactaac gcacagagga tatacgtcac caccggggca gcaacaaacc
    32881 aaagcatgac cgccagcaag ataaacatca gggtcatcac gccacgcgcc cgccagcgaa
    32941 gcggtaatga aggcagttta cccagccagc ctttacgcac caccaggcct ttatgaatac
    33001 gcttactggt acgtccggcg ttcaacgctt tgtacagcgg ctccacagcc gcgatctctt
    33061 catccgtcgc ccgggtacga atcgacatat agccactgat ttttccctcg cgcaccatcg
    33121 gtaccgcatt ggcccgcacc caataatggt cgccattttt gcggcgattt ttcacgatgc
    33181 cgctccaggg ctcccctttt ttcagggtga accacatatc cgcaaacgcc gcttttggca
    33241 tatccgggtg acgcaccatg ttgtgcggct gcccctgtaa ctcttgcaag gtatagccgc
    33301 tcacctgcac aaaagtgtca ttagcatgag tgatatagct ttgcagatcg gtagtggaca
    33361 tcagagtggt atcgtccgcc agcggggtat tttgctgggt gacatacgga tgagaagaca
    33421 tgttcgcgtc ctgtgcaggt tatatggttg ttaacttctt gtcagagttt atgtcggccc
    33481 cgctgcagtt atctttaact gattaatttg atttagatcg caatttgcga ttaaagcata
    33541 aatctaatcc ct
//
LOCUS       DAZFFE010000042        33285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFFE010000042 DAZFFE010000000
VERSION     DAZFFE010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.042"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..242)
                     /gene="pspE"
                     /locus_tag="ACHJ69_002197"
     CDS             complement(<1..242)
                     /gene="pspE"
                     /locus_tag="ACHJ69_002197"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO5015181.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEIL"
     gene            complement(317..538)
                     /gene="pspD"
                     /locus_tag="ACHJ69_002198"
     CDS             complement(317..538)
                     /gene="pspD"
                     /locus_tag="ACHJ69_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO5015182.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(547..906)
                     /gene="pspC"
                     /locus_tag="ACHJ69_002199"
     CDS             complement(547..906)
                     /gene="pspC"
                     /locus_tag="ACHJ69_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO5015183.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(906..1130)
                     /gene="pspB"
                     /locus_tag="ACHJ69_002200"
     CDS             complement(906..1130)
                     /gene="pspB"
                     /locus_tag="ACHJ69_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO5015184.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(1184..1852)
                     /gene="pspA"
                     /locus_tag="ACHJ69_002201"
     CDS             complement(1184..1852)
                     /gene="pspA"
                     /locus_tag="ACHJ69_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO5015185.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKDREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            2019..2996
                     /gene="pspF"
                     /locus_tag="ACHJ69_002202"
     CDS             2019..2996
                     /gene="pspF"
                     /locus_tag="ACHJ69_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO5015186.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPMLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTEHARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPSEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQVSLEQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            3152..3289
                     /locus_tag="ACHJ69_002203"
                     /pseudo
     CDS             3152..3289
                     /locus_tag="ACHJ69_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Putrescine importer PuuP"
     gene            3423..3668
                     /gene="ymjA"
                     /locus_tag="ACHJ69_002204"
     CDS             3423..3668
                     /gene="ymjA"
                     /locus_tag="ACHJ69_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HFO5015187.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            3981..5624
                     /gene="sapA"
                     /locus_tag="ACHJ69_002205"
     CDS             3981..5624
                     /gene="sapA"
                     /locus_tag="ACHJ69_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO5015188.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYAGKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            5621..6586
                     /gene="sapB"
                     /locus_tag="ACHJ69_002206"
     CDS             5621..6586
                     /gene="sapB"
                     /locus_tag="ACHJ69_002206"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO5015189.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEIVMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            6573..7463
                     /gene="sapC"
                     /locus_tag="ACHJ69_002207"
     CDS             6573..7463
                     /gene="sapC"
                     /locus_tag="ACHJ69_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO5015190.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHSLDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            7463..8455
                     /gene="sapD"
                     /locus_tag="ACHJ69_002208"
     CDS             7463..8455
                     /gene="sapD"
                     /locus_tag="ACHJ69_002208"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO5015191.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            8457..9263
                     /gene="sapF"
                     /locus_tag="ACHJ69_002209"
     CDS             8457..9263
                     /gene="sapF"
                     /locus_tag="ACHJ69_002209"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO5015192.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(9311..10474)
                     /locus_tag="ACHJ69_002210"
     CDS             complement(9311..10474)
                     /locus_tag="ACHJ69_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFO5015193.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWVQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(10483..11856)
                     /locus_tag="ACHJ69_002211"
     CDS             complement(10483..11856)
                     /locus_tag="ACHJ69_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFO5015194.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMASAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFAPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(11860..14967)
                     /locus_tag="ACHJ69_002212"
     CDS             complement(11860..14967)
                     /locus_tag="ACHJ69_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFO5015195.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPDTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRTARTMGLYVVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     PRRQE"
     gene            complement(14967..16088)
                     /locus_tag="ACHJ69_002213"
     CDS             complement(14967..16088)
                     /locus_tag="ACHJ69_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO5015196.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYTRLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(16237..16803)
                     /locus_tag="ACHJ69_002214"
     CDS             complement(16237..16803)
                     /locus_tag="ACHJ69_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO5015197.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            17281..18069
                     /gene="fabI"
                     /locus_tag="ACHJ69_002215"
     CDS             17281..18069
                     /gene="fabI"
                     /locus_tag="ACHJ69_002215"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO5015198.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            18214..19341
                     /gene="yciW"
                     /locus_tag="ACHJ69_002216"
     CDS             18214..19341
                     /gene="yciW"
                     /locus_tag="ACHJ69_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO5015199.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVCDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFATCMDSSNEYKKISALLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            19409..21343
                     /gene="rnb"
                     /locus_tag="ACHJ69_002217"
     CDS             19409..21343
                     /gene="rnb"
                     /locus_tag="ACHJ69_002217"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO5015200.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DGKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSTDGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(21420..21524)
                     /locus_tag="ACHJ69_002218"
     CDS             complement(21420..21524)
                     /locus_tag="ACHJ69_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015201.1"
                     /translation="MTYMKLTAAKHIVFFIYLFVIQFWKNASFQTKVS"
     gene            21578..23563
                     /gene="pdeR"
                     /locus_tag="ACHJ69_002219"
     CDS             21578..23563
                     /gene="pdeR"
                     /locus_tag="ACHJ69_002219"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO5015202.1"
                     /translation="MKTVRESATLYNFLGSHNPYWRLTESSDVLRFSTTEITEPDRIL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWGGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEALA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSRLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(23545..23673)
                     /gene="ymiD"
                     /locus_tag="ACHJ69_002220"
     CDS             complement(23545..23673)
                     /gene="ymiD"
                     /locus_tag="ACHJ69_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO5015203.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYARFR"
     gene            23711..23884
                     /gene="yciZ"
                     /locus_tag="ACHJ69_002221"
     CDS             23711..23884
                     /gene="yciZ"
                     /locus_tag="ACHJ69_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO5015204.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            23974..24723
                     /gene="yciT"
                     /locus_tag="ACHJ69_002222"
     CDS             23974..24723
                     /gene="yciT"
                     /locus_tag="ACHJ69_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO5015205.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEQSKLTVHVVDI"
     gene            24992..25210
                     /gene="osmB"
                     /locus_tag="ACHJ69_002223"
     CDS             24992..25210
                     /gene="osmB"
                     /locus_tag="ACHJ69_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO5015206.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(25336..25662)
                     /gene="yciH"
                     /locus_tag="ACHJ69_002224"
     CDS             complement(25336..25662)
                     /gene="yciH"
                     /locus_tag="ACHJ69_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO5015207.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVALDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(25662..26399)
                     /gene="pyrF"
                     /locus_tag="ACHJ69_002225"
     CDS             complement(25662..26399)
                     /gene="pyrF"
                     /locus_tag="ACHJ69_002225"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO5015208.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAAMAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(26591..27760)
                     /gene="lapB"
                     /locus_tag="ACHJ69_002226"
     CDS             complement(26591..27760)
                     /gene="lapB"
                     /locus_tag="ACHJ69_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO5015209.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(27767..28075)
                     /gene="lapA"
                     /locus_tag="ACHJ69_002227"
     CDS             complement(27767..28075)
                     /gene="lapA"
                     /locus_tag="ACHJ69_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO5015210.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSASKE
                     "
     gene            complement(28224..28988)
                     /gene="pgpB"
                     /locus_tag="ACHJ69_002228"
     CDS             complement(28224..28988)
                     /gene="pgpB"
                     /locus_tag="ACHJ69_002228"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO5015211.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWLSGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAIMLFAILAAAILMGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            29158..29748
                     /gene="ribA"
                     /locus_tag="ACHJ69_002229"
     CDS             29158..29748
                     /gene="ribA"
                     /locus_tag="ACHJ69_002229"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO5015212.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIIERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(29812..32487)
                     /gene="acnA"
                     /locus_tag="ACHJ69_002230"
     CDS             complement(29812..32487)
                     /gene="acnA"
                     /locus_tag="ACHJ69_002230"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO5015213.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGKSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQIELVEKYAKAQGMWRNPGDEPIFTSVLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASSELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVDQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLELTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTTTELTYYQNDGILHYVIRNMLK"
     gene            complement(32637..32732)
                     /gene="ymiC"
                     /locus_tag="ACHJ69_002231"
     CDS             complement(32637..32732)
                     /gene="ymiC"
                     /locus_tag="ACHJ69_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO5015214.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFCAELLWIITH"
     gene            complement(32846..33013)
                     /gene="yciX"
                     /locus_tag="ACHJ69_002232"
     CDS             complement(32846..33013)
                     /gene="yciX"
                     /locus_tag="ACHJ69_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO5015215.1"
                     /translation="MVGQEQLESSPLCQHSDNEPEAKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(33016..33156)
                     /gene="ymiA"
                     /locus_tag="ACHJ69_002233"
     CDS             complement(33016..33156)
                     /gene="ymiA"
                     /locus_tag="ACHJ69_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO5015216.1"
                     /translation="MRLAMPSGNQEPRRNPELKRKAWLAVFLVSALFWVVVALFIWKL
                     WG"
BASE COUNT         8206 a         8686 c         8072 g         8321 t
ORIGIN      
        1 ctaaggatct cttttgcctg ccctgactgg cgtccggcat tgcaatacac tttcacggtg
       61 tcgtttttat ccggaacggc ggtggcaatg cgctctttca cttccttcaa gggaatattg
      121 atggccccct gaacgtgttc ttgctgatac tgctctggaa cacgaacatc gatccagtgt
      181 tcagcggcga aaacgggcag tgaaaacacc agtgtcagag caagtaagcc ttttttaaac
      241 atgtaagcgt ccttctatgg atgaaaattg ctatccatta tacgcctgag atttaaccat
      301 ttttgcgaaa ctcccctcac cttttataac gctgcgccag tttattggca gcccgactta
      361 acagcggttc cagcgccaca gccagcaaca ttttcagcgg tcggcgagca actgatttta
      421 tcgcccagcc cgccacaccc gccgggccat agcgcagtgc ggtaagaagt accagcttgc
      481 ctgctaattt gaacccaggc ttcacctttt gcccggcctg ttgccagcga gtattcataa
      541 ctttcctcac agttgacgga aacggctacg taacgtgaaa gtatcggaag tgacataacg
      601 ttccatctcg cgtaaacgcg tttcacttgc cgccagttca cggtcgactt catccagcaa
      661 ttcgctgctg gaaggtagct gctcaccaaa ggccatgttg tccggcattg gatcaagcgc
      721 aaatgacaaa atgatgtaag caaccagggt aaacagcgcc agaccgaaga aaatcgacag
      781 caccaccagg atacgcacca gttttactgg tacatcaaaa tagttggcaa tcccggcgca
      841 gacgccacgg accatgccct gctgcggaat acgccataat tttttattga gattaatgcc
      901 cgccattagc gatccctcca gttcggatgt tctgcatcaa gaatagattc cagcgcctga
      961 atacgttcgc gcatccgttt tgcttcatca gccagttgcg ctaatcgctg ctgctcactt
     1021 tgcgacaatt cactgcgacc agaacgattg ctgtaatgca gccataacca gatcggtaaa
     1081 acaaacagca caaaaatggt taacggaata gccagaaata gcgcgctcat aaggactcct
     1141 tagacgatga gcggcggcgc gttcggacgc cgcctggatg ttattattga ttgtcttgct
     1201 tcattttggc ttttaattgt gccagttgct cgctgattgc atcatcggct ttcagttcgg
     1261 caaactgatc gtccagcgat ttttgtttac cgaagctgtg gctttctgct tccgcttcca
     1321 tctggtcaat tcgacgttcg aaagattcga aacgagccat tgcttcatcc agtttgccac
     1381 tgtccagctg acgacgcaca tcgcgcgacg agtttgccgc ctgatggcgt aacatcaatg
     1441 cctgctggcg agcgcgtgtt tcactcaatt tgttttccag ctcgccgatc tctttcttca
     1501 tgcgtgccag cgtatcgtcc accagcgtca cttcatgttc caggctctta atcagatcgg
     1561 tcagtttctg tttttcaatt aacgccgcac gcgccaggtc ttctctatct ttcagcagtg
     1621 ccagttcggc tttttcctgc cattcaactt cacgcgccga cgcttgttca atacggcgag
     1681 tcagctgttt cttttctgcc agcgcacgcg ccgaagtaga acgtacttca accagtgtat
     1741 cttccatctc ctggatcatc agacgcacca gtttctgtgg atcttccgct ttctctaaca
     1801 gagcgttgat gttggcattc acgatgtcgg caaagcgaga aaaaataccc ataatgttgt
     1861 cctcttgatt tctgcgttca ggattgtcct gctgaactgt taatacaatt tgcgtgccaa
     1921 ttttttatct ttttgattta taaagatctg attgaagaat caacagcaac atgccaggat
     1981 gaattagcga attacactaa caagtggcga atttcatcat ggcagaatac aaagataatt
     2041 tacttggtga ggcgaatagc tttctcgaag tgctggaaca ggtttcgcat ctcgcaccac
     2101 tggataaacc gatgctcatc atcggcgaac gcggcaccgg taaagagttg attgccagcc
     2161 gcctgcatta tctctcctcc cgttggcaag ggccgtttat ttcccttaac tgcgcggcgt
     2221 taaatgaaaa tctgctggat tccgaactgt ttggtcacga agcgggggcg tttaccggtg
     2281 cgcaaaaacg tcatcctggg agatttgaac gtgccgatgg cggtacgcta tttcttgatg
     2341 aactcgctac ggcaccgatg atggtgcagg aaaaattatt gcgtgtgatt gagtacggtg
     2401 aactggagcg cgttggcggt agccagccat tgcaggtgaa tgtgcggttg gtatgcgcga
     2461 cgaatgccga tctccctgcg atggtcaatg aaggtacttt tcgcgctgac ctgctcgacc
     2521 gactagcgtt cgatgttgtg caactgccgc cactacgcga acgtgaaagc gacattatgt
     2581 taatggcaga acactttgcc atccagatgt gtcgggaaat caagctgcct ctgttcccgg
     2641 gttttaccga gcacgccaga gaaacattac tgaattatcg ctggccggga aatatacgtg
     2701 aattgaaaaa cgtggtggaa cgttcagtgt atcgccacgg caccagcgat tatccgcttg
     2761 atgacatcat tattgatccc tttaaacgcc gtccatctga agaagccatc gccgtttcag
     2821 aaaacacttc gcttccgacg ctgccactgg atttacgcga gtttcagatg cagcaggaaa
     2881 aagaactgct tcaggtcagc ttggaacaag ggaagtataa ccagaagcgc gccgctgaat
     2941 tgctggggtt aacctatcat cagttccgcg cgttgttgaa aaagcaccag atttagcgaa
     3001 gttgggcaat acaacttgcc ggatgcagcg tgaacgcttt atccggcata cataatcaat
     3061 acgtcgtttg caggcctgat aagcgctgcg catcaggcat tattgcgtta accctgcttc
     3121 tgcgtattaa tctctcagcg catcctgcaa aatttgcatt gccgcatcga attgcgcaac
     3181 cgggatggta tgggcttcgt taggcggcgc atatttgtgg tatttgatcc gccgctatcg
     3241 caaagtgccg ttgtatgatg gcgacagaac gccggtgagc gaaacgtaat agtcgaatgc
     3301 gcggcgcatt atcctgcctg agggtaatgc accgcgccat taactataaa aatatgtatg
     3361 acccggatca cgagtgctat catccgcctt tatgcttagc gcattaaccc caggggcaaa
     3421 taatgaacca cgatatcccg ctaaaatatt ttgatattgc cgatgagtac gcgaccgaat
     3481 gcgcagagcc tgtcgcagac gctgaacgca caccactggc ccactacttc cagttactgc
     3541 tcacccgttt gatgaacaat gaagagatca gtgaagaagc ccaacatgaa atggctgctg
     3601 aagcggggat taatcccgta cgcattgatg agatcgcaga gtttttgaat caatggggta
     3661 atgagtaatt atttaattgc agacgatcgg tcagtatcgt tgcccggata aagcgttcgc
     3721 gccgcatccg gctaaaaatg acacgcacag cagcgtgctt taatgggttt ctcacgatcg
     3781 taccgctctg ctcctagcta agttgttctg tgagttatag ttataaagaa atcacgggta
     3841 cgaatgttga actgatggcg acgccacctg ttcaatgacc aaccacacgg ccctcattac
     3901 gacctctgaa cacaaacggt aatggcaaat gtcattaaat attagtaaga tagcctcttt
     3961 acataaaatc ccttaatatt atgcgccagg tattatcgtc tcttttggtg attgctggac
     4021 ttgtgagtgg tcaggcaatc gccgcgcctg aatccccccc gcatgctgat atccgcgaca
     4081 gcggttttgt ctattgcgtc agcgggcaag tcaacacctt taacccatcc aaagcgagca
     4141 gtgggttaat tgtcgatacc cttgccgccc agttttatga tcgactgctg gatgtcgatc
     4201 cctataccta tcgcctgatg ccggaacttg ccgaaagctg ggaagtactc gacaacggcg
     4261 cgacctatcg cttccacctg cgtcgcgatg ttccgtttca aaaaaccgac tggtttactc
     4321 ccactcgtaa aatgaatgcc gatgatgtgg tgtttacctt ccaacggatt tttgaccgca
     4381 acaacccgtg gcataacgtc aacggcagta acttccccta tttcgacagt ctgcaatttg
     4441 ccgataacgt gaaaagcgtc cgcaagctgg ataatcatac cgttgagttc cgtctcgccc
     4501 agccggatgc ttcttttttg tggcacctgg cgacccatta tgcttcggtc atgtcggcag
     4561 aatatgcagg gaagttagag aaagaagatc gccaggagca actcgaccgt caaccggtcg
     4621 gcaccggtcc gtatcagttg tcggaatacc gcgccgggca atatattcgc ttacaacgtc
     4681 atgatgattt ctggcgtggt aagccgttga tgccacaagt ggtggtagat ttaggctctg
     4741 gcggtacagg acgattgtcg aaactcctga ccggggagtg cgacgttctg gcctggcctg
     4801 ctgccagtca gctatccatt ttgcgtgacg acccacgctt gcgtttaacg ctgcgtcctg
     4861 ggatgaatgt cgcctatctg gcattcaaca ccgccaaacc gccgctaaat aatccggcgg
     4921 tccgccatgc gctggcactg gcgattaata accagcgcct gatgcaatcc atctattatg
     4981 gtacggctga aacggctgcc tctattttac cgcgagcctc gtgggcctat gacaacgagg
     5041 ctaaaattac tgaatacaat ccggcaaaat cgcgcgaaca gttgaaggcg ttggggctgg
     5101 aaaacttaac gctgaaactg tgggtgccca cacgttcgca ggcgtggaac cccagtccac
     5161 tgaaaaccgc cgaactgatt caggcggata tggcacaggt tggcgtaaaa gtggtgattg
     5221 tgccggtaga aggtcgtttt caggaggcgc ggttgatgga tatgagccat gatctgacgt
     5281 tatccggttg ggcgacggac agtaacgacc cggacagttt cttccgtccg ttactgagct
     5341 gcgcagcaat tcattcgcag accaacctcg cccactggtg cgatccgaaa ttcgacagcg
     5401 tgttgcgtaa ggcgctctct tcgcagcagc tggcggcgcg tattgaagcc tatgacgaag
     5461 cgcaaagtat tctggcgcag gaattaccta ttctgccgct ggcgtcgtca ttgcgtttgc
     5521 aggcctatcg gtacgatatc aaaggtctgg tacttagccc gtttggtaac gcctcctttg
     5581 ctggggtgta tcgcgagaaa caggatgagg tgaaaaagcc atgattatct tcaccttacg
     5641 ccgtattttg ttattgattg tgaccttgtt cctgctgacc ttcgttggct tcagcttgag
     5701 ctatttcacc ccacatgcgc cgttacaagg cgcgtcactg tggaatgcct gggtgttctg
     5761 gtttaacggc ctgatccact gggattttgg tgtttccagc attaacggtc aaccgattgc
     5821 cgaacagtta aaagaggtct tcccggcgac gatggaactg tgcatcctcg ccttcggttt
     5881 tgcgctgatt gttggcatcc ccgtaggaat gattgccggg attacgcgcc ataagtggca
     5941 ggataacttg atcaacgcca tcgccttact gggcttttca atcccggtgt tctggctggc
     6001 gcttctgctg acgctgtttt gttcactcac gctaggttgg ttgcccgttt ctgggcgttt
     6061 cgatttgctc tacgaagtga aaccgattac cggttttgcg ttgattgatg cctggctttc
     6121 ggactcaccg tggcgggatg aaatagtcat gagcgcaatc cgccatatga tattgcccgt
     6181 gatcaccctg tcggttgcgc caacaactga agtgatccgg ctaatgcgta tcagcactat
     6241 cgaagtgtat gaccagaatt atgtaaaagc agcggcgacc cgcggtttgt cgcgctttac
     6301 tattttgcgt cgccacgttc tgcataacgc gttacctccg gttattcctc gcctgggctt
     6361 acagttttct accatgctga ctctggcgat gatcaccgaa atggttttta gctggccggg
     6421 tttagggcgc tggttaatta acgccatccg ccagcaggat tacgcagcca tttccgccgg
     6481 agtgatggtg tgtggctcac tggtcattat tgttaacgtg atttctgata ttttgggtgc
     6541 catggctaac cctctgaaac ataaggaatg gtatgcctta cgatagcgta tacagcgaaa
     6601 agcgcccgcc gggcacgctg cgtaccgcct ggcgcaaatt ttatagtgac gcctctgcga
     6661 tggtcggcct gtacggctgc gcggggctgg ctgtactgtg tatttttggc ggctggtttg
     6721 cgccttacgg catcgaccag caatttctcg gttatcaatt attgccgccg tcatggtcgc
     6781 gctatggcga agtttctttc tttctgggga cggacgacct cggacgcgat gttttaagcc
     6841 gtttactgag cggagctgcg ccaaccgtag gtggcgcatt tgtggtgacg cttgccgcga
     6901 cgatctgtgg cctggtactg ggcaccttcg ccggggcgac ccacggccta cgctcagcgg
     6961 tgcttaacca tagtctggat accttactgg cgatcccttc gctgctgctg gcaattatcg
     7021 ttgtagcgtt tgccggaccg agtttgtctc acgccatgtt tgctgtctgg ctggcattgc
     7081 tgccgcgtat ggtacgttcg atttacagca tggtgcatga cgaactggaa aaagagtacg
     7141 ttatcgccgc ccgtctggat ggcgcatcaa cgctgaatat tctctggttt gctgtgatgc
     7201 ccaacatcac tgccggactg gtcaccgaga tcactcgcgc attgtcgatg gcaattctcg
     7261 atatcgccgc gctgggcttt cttgacctcg gcgcacaact gccctcgcct gaatggggcg
     7321 cgatgctcgg tgatgcgctg gaactgattt atgtcgcccc atggactgtc atgctgccag
     7381 gtgcggcaat tatgattagc gtgctgttgg ttaacctcct tggcgacggt gtccgtcgtg
     7441 caattattgc gggggtggaa taatgccatt actcgatatt cgtaacctga ccattgaatt
     7501 taaaaccggt gatgagtggg ttaaagccgt cgaccgcgta agtatgacct taaccgaagg
     7561 tgaaatccgc ggtcttgttg gcgaatcagg ttccggcaaa agtttgattg cgaaagcgat
     7621 ttgtggggtg aataaagata actggcgtgt tactgctgac cgtatgcgtt ttgatgatat
     7681 cgatttgctg cgtctctccg cacgcgaacg gcgcaagctg gttggtcaca acgtgtcgat
     7741 gattttccag gaaccgcagt cgtgtcttga cccttcagaa cgtgtgggtc gccagttgat
     7801 gcaaaacatc cctgcctgga cctataaagg ccgttggtgg cagcgttttg gctggcgcaa
     7861 acgccgtgcc attgaactgc tgcaccgcgt ggggattaaa gatcacaaag atgcgatgcg
     7921 cagttttccc tatgagttga ccgaaggtga atgtcagaaa gtgatgatag ccattgcact
     7981 ggcgaatcaa ccgcgtctgc taattgctga cgaaccgacc aactcaatgg agccaacaac
     8041 ccaggcgcaa atctttcgcc tgctgacgcg tctcaaccaa aacagcaata ccactatttt
     8101 gcttatcagc catgacttac aaatgcttag ccaatgggcg gataaaatta acgtgcttta
     8161 ctgcggtcaa acggtggaaa ccgcgccgag taaggagttg gtgacgatgc cacaccatcc
     8221 ttatacccag gcgctgatcc gcgcgatacc agacttcggc agcgcgatgc cgcataaaag
     8281 tcgcctcaat acgctgcccg gcgctatccc gctgctggaa cagttaccga ttggttgtcg
     8341 tctggggcca cgttgcccgt atgcacaacg agaatgcatt gtgacgccac gtttgacggg
     8401 ggcgaaaaat catctctatg cctgtcattt cccgctgaac atggagaaag agtgagatga
     8461 tcgaaacgct acttgaagtg cgcaatttaa gtaaaacgtt ccgctaccgg accggctggt
     8521 ttcgtcgtca gaccgtagaa gcggtaaaac ccttgagctt tacgctacgt gaaggccaga
     8581 cactggcgat tatcggcgag aatggttcgg gtaaatccac gctggcaaaa atgctggcgg
     8641 gaatgataga accgaccagc ggcgagttat tgattgacga tcatccgctg cattttggcg
     8701 attattcctt ccgcagtcag cgtattcgca tgatttttca ggatccttcg acctcgttga
     8761 atccccgtca acgtatctcg caaattctgg attttccact gcgcctgaac accgacctgg
     8821 aaccggaaca gcgtcgcaaa cagattattg aaacgatgcg tatggttggg ctgctaccgg
     8881 atcacgtcag ttactatccg catatgttag cacccgggca aaaacagcgt ctgggtctgg
     8941 cgcgcgcgct gatattgcgc ccaaaagtca ttattgccga tgaagccctc gcctcactgg
     9001 atatgtcgat gcgttcgcag ttgattaatc tgatgctgga gttacaggaa aaacagggca
     9061 tttcgtatat ttatgttacc cagcatatcg gaatgatgaa gcacattagc gaccaggtgc
     9121 tggtgatgca tcagggcgag gttgtcgagc gaggcagcac cgcagatgtg ctggcatcgc
     9181 cgctgcatga actcaccaaa cggctgatcg ccggtcattt tggcgaagca ttgacggcgg
     9241 atgcgtggcg taaggaccgt tagtgttttg cacctcatcc tgacaggccc ccctcaggat
     9301 gaggtgttca ttacttttga ttgtcgattc tggtgagagc aaccaacaat gccagcgtat
     9361 aacacaccgt aattgccaga ctcattttta acagtgtttc tccgccaatt cccgacaatg
     9421 gcgcggctat accgccgaag acaaacatgc tcatccccat caacgcagac gccgttccag
     9481 attcctgcgt ccctactgca ctcatagcct ctgccccgcc aacagtgccg atgccgctac
     9541 aaaatgcgat ggtgaaaaat aatgccacca gtgccggaat gggcaattgt acccatgcgc
     9601 ataagaccgt gagcaatgca cacaaaattg ccgctatcag gccacctcgc aacaatgtca
     9661 tcgcgttaat ccgtcgcgcc aggcgcgaga agatccatga agcaatgata agtccgatgc
     9721 cgttaaggcc aaacaccagg ctaaattgca ttggactaaa gccaaattcc ttctgcaaca
     9781 cgaacgacga agagccgata tatgcaaaca aaccagccag cataaaactt tgaatcaggc
     9841 aaaagcgcat aaaacggcgg ttctgcacca cgcttcgtcc ggttaatagc aatgatgagc
     9901 cccttttatt ttctggcaag gtctcattaa taaataacag gcagcccagc aacagtacgg
     9961 tgctaatttc agccattacc cagaataaag tacgccagtc aaaagtgcta acaatgtacc
    10021 cgcccagcac tggcgacaac accggtgcca ggccattcac cgtcattaac agcgcaaaaa
    10081 actgggtcaa cgttactccc tgatatttgt cacgagcaat agaacgagag agcaccgagc
    10141 cacccgcccc cgcgatccct tgaataaaac gccagaccac cagccagtaa atattgttcg
    10201 ttgtcgcgca taaaatggaa gaaacaataa acaatagcaa cgacaagatt aacggacgtt
    10261 tacgccctat tttgtcgctc agagggccaa ataacagttg tccgacgcca agaccaatca
    10321 gtgatgcggt cagggttaat tgcgttatgg tcgtggttgc cgccagttgc tcagacatct
    10381 ccggcaaagc cggcaaataa agatcggtac acatcgggcc gataccggct aacagaccaa
    10441 gaatcaatgc ccatgaaaga gagactctag ccatattatt ccttacttga gtgatgacat
    10501 gccgccgccc agcgactgcc agagcgtgat tcggtttgta aaatcagtca gttgcagtgc
    10561 cagcagctcc tgctgtgcgc tccacaagct gcgctgcgcc gtcaacacgg tcaggtaatc
    10621 accaacaccc gcctgatagc gacgcaagcc aacatcaacc gtttgttgct cagctttaac
    10681 atactggcgc tgtgcatcca gttgctcttc aagcgtggtc cgccgcgcca gtgcgttagc
    10741 gacatcttta aatgcgctct gaacggtttt ttcataggtg gcaatgagcc cacgcttttg
    10801 tgcttctgcg taacgaagct gcgccagatt acttccgcca gtgaacagcg gtaatgtgac
    10861 cgatggcgca aatgaccaga tctgcattcc gtggctgaac aacgacgaca atgcgtcact
    10921 gccaacaccg gcactggcag tcagcgaaat cgtcgggaaa aagttagccc gcgccgcgcc
    10981 aatatctgca ttggcgctct tcaaattatg ttcagcttcc tggatatccg ggcgacgcag
    11041 cagaacatca gatgatactc ccgcaggaac cagacttatc atttgttccg gcaggctttc
    11101 cagtgttccc ggtagcagat tttccgcaag cgttgtacct gccagtaagt tcagcgcatt
    11161 tttatcctgc atcacttgtg tctgatagct ggcaacgctg gcgcgcgctt gctgataaac
    11221 actcatcgcc tcactgacat ctgtcgctgc cgccgtgcca acctgttgct ggcgctggat
    11281 aattttcaat gagttttctg cactggccat cgtttctttt gccagggcca ggttgctgtt
    11341 atcggccgcc agtgtcagcc aggcggtgct gatttcagca atcagcgtta aacgagtgtt
    11401 ctgggctgta aattcactgg caagccaggt ttcccgcgcc gcccgagata aactctgatt
    11461 acgaccaaac agatctagcg tataactgga caccgtgccg tcagcctcgg cagttgttcc
    11521 cgtaccattc gctagcgaac gactgcgagt cgaacttaag gccgcattca ccgtcggaaa
    11581 taacgacgca ttagtttgcc catagagcgc ccgtgctgag tcaatatcag caatcgcttt
    11641 ttgcacatcg cggttactgt tcagtgcaat ggttactacc tgctgcaatc ggggatcgtg
    11701 gatcacctgc tgccagtcat ggctgattgc cttcccctgg ccctgtgctc ccggcaaggt
    11761 cgccgggatt ggggattcag gcgtactgta gtgaggatcg agcgagacac atcccgcact
    11821 caacaacacc agaaaaataa gagaacgacg caacataact tactcctgac ggcgcggttt
    11881 accggcaaat aaacgtttca ccagtacaaa aaacagagga acgaagaaaa tggccagtaa
    11941 tgtagcggtc agcgtgccgc caataatgcc cgtaccaatg gcgatgcggc tgttcgcccc
    12001 tgcgccggtt gctatcgcca gcggcattac ccccgcaata aacgccagcg aggtcatgat
    12061 gattgggcgt aaacgagtct gcgccgcgcg taatgccgca cgactcaagg aatagccctc
    12121 tgcaaccgcc gcttcagcaa attccacaat cagaatggcg tttttcgacg acaagccgat
    12181 agtagttaat agcgctacct ggaagtaaac gtcgttgttt aaatcacgca tccaggccgc
    12241 cagcgccgcg ccaagaagcc ccagagggat caccagaatg accgagaacg ggactgacca
    12301 gctttcgtac agtgctgcaa ggcagaggaa aaccaccaga atagaaactg catacagact
    12361 taatgcctga ccgctggcca gtttctcctg caatgacaaa ccactccagg cccaggttgt
    12421 tcccgcagga agactgtttg ccagttcctc catttttgtc attgcatcgc cggaactaaa
    12481 gccagtcgcg ttttcgccct gaatttcata ggctgccgag ccgttgtagc gcaccagtct
    12541 ttccggtcca tacagccagc gggtggtcgc aaaagcagag aatggtgtca tggcgttatc
    12601 gctaccgcgc acaaaccatt tacctaagtc tgacggcgcg gaacgaaatt cgctgtcgcc
    12661 ctgaatgtag acttttttca cacgaccacg gtcaataaag tcattcacat aagtaccgcc
    12721 ccacgcactg gacagggtgt cggtgacatc attcaaactc aaccctaatg acaccgcttt
    12781 attgctatca atatctacct gcaattgcgg catttgtggc aaatcattgg cgcgtacaga
    12841 atgtaactcc ggactttgat tcgctttttc tatcaactga ttacgcattt gcagcagtgt
    12901 ttcacgatcg gttccaccgt tagctaacaa ttcaaacgta aaaccattgc tttgccccag
    12961 cccatcaacg gctggcggcg tcatcgcaaa taccgtggca tcacgaattg tgcccagctc
    13021 tttggttgcc cgtagggcga tagcctgggc ggtgttttct gcccctttac gttgagacca
    13081 gtttttcaac gaaacaaacg ccatcccggt gttctgtccg ctgccgctaa aactgaaacc
    13141 atcaacggta aagatgacat cggtatttgc tttctcgtta atcaggaacc agtcaacaat
    13201 ctggcgattc acttctgctg tacgggcagc ggttgcaccg gccggcagcg tgtactgcac
    13261 catgatttcg ccctggtctt cagtgggtaa gaaactgccc ggcagtttcc acatcatcag
    13321 ggccattcca ccacccagca cgacataaag ccccatcgtt cgggctgtac ggcgcaggac
    13381 ataaattacg cctcgctgat atttatcttc agtacggcgg taaaagcggt taaatgcgcc
    13441 gaaaaagcct tttttatgtg gtggaacatg ctggaggacg gaaccgcaca gggcgggagt
    13501 caaggtcaat gccaccacca cggaaagcag cattgcggag atgatggtga tggagaactg
    13561 acgataaatt acccccgtag atcccccaaa gaaggccatc ggcaggaata cggctgacaa
    13621 caccagcgca atggcaacca gtgcgccaga aatctcgccc attgattttt ccgtggcttc
    13681 acgcgcgggc aacccttcgt cacgcataat gcgttcgacg ttctctacca ccacaatggc
    13741 atcgtccacc agcaagccga tcgccagcac cattgcaaac aacgtcaggg tattaatcga
    13801 atagccaaac aacgcaagaa cgccgaatgt gcccagcaaa acgacgggga cagctaacgc
    13861 cggaatcagt gtggcacgca ggttttgcag gaataaatac atcacgcaaa ccaccaatac
    13921 gatggcttca aacagcgtct gaattacgtc ctctacggag attttaataa attcagtgct
    13981 gtctttcggg tatgcgatgt cgtatccctg cggcatgttc cgctggaatt cggcaatctt
    14041 atccttgacc agcgtcgccg tattcagcgc attcgcaccg ggcgaaagca ttacagcaac
    14101 cccggcagac gggtgaccgt taagtttgcc gatagcggta taatcttcac tgcccatctc
    14161 aacgcgagcc acatctttta tacgaacaac tgcgccgtct gactggcttt tcacgataat
    14221 atttttgaac tgatccaccg tctgcaaacg agactgcgca cgtaccgttg cggtcagttg
    14281 ctgagtatcc ggtgaaggca atgcccctat tttcccggca gtaacctgca cgttttgcgc
    14341 ttcaatagca ctttgcacgt ctgaaggcat cagcgagtaa gacgccagtt tggccgggtc
    14401 gagccagatg cgcatagcgt attccgcgcc aaagacttgc agactcccca caccttcaac
    14461 acgcgccagg gggtcctgaa cgttactgac cagccagtcg gcgatatccg aactggaggc
    14521 tttgtcagtg gtgtcataca cggcagcaat cagcaaaaag ttgctttgtg atttctccac
    14581 cgtaacaccc gtttgctgca cttcgctggg taggcgcgac tccgcctgct gaattttatt
    14641 ctgcacctga acctgtgcag tatctggatc ggtaccttgt tcaaaggtca cattaatact
    14701 gaccgaacca tcagagctac tggttgagct gaaatagagt aaattatcaa gcccagtgag
    14761 ttgttgctcg ataacctgag tcacgctgtt ctccagcgtt tcggcagaag caccagtata
    14821 agtggctgaa attttaatgg tcggcggtgc aacgtcagga tattgcgcga cgggcaatgt
    14881 gcgaatagcc agaatccccg ccaacataat caaaatggcg ataacccagg caaagaccgg
    14941 gcggcgaaca aaaaagcgcg aaaacatcag gcgttgcctc cattagcctg aacttcaaca
    15001 gccttgacgg tctgccctga agtgactttg gcagaacctt caacaatcag tcggtcgccg
    15061 ttgtgcaggc cgttcagcac cagccattta tcaccatacg tttctcccgt ttcgagcgtt
    15121 cgctgctcta ctttattgtc tttattcacc accagcgcag ttgcattgcc tttagcatcg
    15181 cgcgtaacgc cctgttgagg cgcgagaata gcgtcttcca tcacgccttc atcgacacga
    15241 gcgcgaacaa acattcccgg taataactgc tgttgtggat tggggaaaat tgcccgtaat
    15301 gtcaccgaac cggtagactc atccaccgcg acttcggtga gttccagacg ccctttttcg
    15361 ctgtaggttg tgccatcttc cagaataagt gagacgctca tggtgtcact gttggtcgcc
    15421 agtgactgtt tacgcagacg taataaatcg acactggagc gagtgaggtc gacatacatt
    15481 gtatccagac cacgaatagt cgttaacgct gtatcctgcg acgcggtcac cagtgcgcca
    15541 ggggttaccg acgaaatgcc aatacgcccc gaaatcggtg cggttaccgt ggtccagtca
    15601 agattaatgc gcgcagtttc cagtgcggct tttttcgcct ctacactggc tttatcttgt
    15661 gcacaggtag actgagcatc atcagcatcc tgttgtgaaa caccgttctc tttcaccagt
    15721 cgggtataac gctgcgcttt ctggcaatcg gcttttacca gtgcctgtgc ttgttgtaat
    15781 gctgcccggg cttcattcca tgcagcctga taactggccg catcaatctg gtagagaggc
    15841 tgtccagcct tgaccagatc accttcctta aataagcgtt tctggataat tcccccaacc
    15901 tgcggacgta cttcagcact gagcgcagca ctggtacgtc cggttaattc actgactacc
    15961 gaaaccggtt gagatttgag cgtaacaacg ccgacttctg tttctgacgg ggatgaattg
    16021 ttactttgcg tattatcaca acccgataag agcagcattg ccactacaga tgttgctata
    16081 tacttcatta ttttctccag ggtgagaaat accctcctca ttgaaattaa tgaggatatg
    16141 tcagggatat tcagttataa cgaacccgca tcgcgaaatg aatttcgcaa tgaaataagg
    16201 gggattaata tcccccgcta ttggttaatt ctgttcttat ttatttatca acatactaac
    16261 aatatcctga tatatttcct gcagatgttg tggatcgctt ttttgcggtg ttaaacgacg
    16321 ataaaccgta ccttccatca tggtagcaaa cacttcaacg cagcaacgaa tgtgctcgtc
    16381 gctaagatgg ggatgcattc ttttcatatg tgcacaaccg ttagcgaaca ttctggcgtc
    16441 agcttcttca agcattgcca taacccgcgg gttgcgtgtg gcttctgcag ccacttccag
    16501 catcagcgcg tcgtcgtcct catttaagga tttacgcaag gcaaggactt ccgggaagtg
    16561 atcggtacgc gcgtcaatgt ccatttgggc aatacgaaag tcgatgatac gacgaaccat
    16621 ctcttcaata atggcgtctt tgttggcgaa gtaacgatat atctgaccca cgctaagctg
    16681 agcttcgctg gcaatttgcg acatactggc ggcatgaaat ccgcagcgac gaaaacaccg
    16741 acgggcggcg ttaatgattt catcctgacg ctgtttgtgg cgtatctcca gcttgctggt
    16801 catttctgtc tcctggccta tcacttgatg agaatgatcg ttctcatttt tgctaattat
    16861 acgtttctcc aaagtaatgg aaagtatttt tatcttattc atgatgcata ttctgataat
    16921 gaagagattg tggagattct gtgaagatag ttgacaatat taagttgggg tcaattcatc
    16981 agaaagacag cgacttgttg tcgccagttc tgttttcatc atcaaattta tcggagctaa
    17041 tacgaatagt ttcatagtca gattctataa gatatttccc caacctcccc cttgcaggcg
    17101 tcaggagtag ataacgcaaa atgctctgta ccgcagcttc tctccgctac agaaagcgca
    17161 actatagcca cccacagcca ggttgattat aataaccgtt tatctgttcg tactgtttac
    17221 taaaacgacg aatcgcctga ttttcaggca caacaagcat caacaataag gattaaagct
    17281 atgggttttc tttccggtaa gcgcattctg gtaaccggtg ttgccagcaa actgtccatc
    17341 gcctacggta tcgctcaggc gatgcaccgc gaaggagctg aactggcatt cacctaccag
    17401 aacgacaaac tgaaaggccg cgtagaagaa tttgccgctc aattgggttc tgacatcgtt
    17461 ctgcagtgcg atgttgccga agataccagc atcgacacca tgttcgctga actggggaaa
    17521 gtttggccga aatttgacgg tttcgtacac tctattggtt ttgcacctgg cgatcagctg
    17581 gatggtgact atgttaacgc cgttactcgt gaaggcttca aaattgccca cgacatcagc
    17641 tcctacagct tcgttgcaat ggcaaaagct tgccgctcca tgctgaatcc gggttctgcc
    17701 ctactgaccc tttcctacct tggcgctgag cgcgctatcc cgaattacaa cgttatgggt
    17761 ctggcaaaag cgtctctgga agcgaacgtg cgctatatgg cgaacgcgat gggtccggaa
    17821 ggtgtgcgtg ttaacgccat ctctgctggt ccgatccgta ctctggcggc ttccggtatc
    17881 aaagacttcc gcaaaatgct ggctcattgc gaagccgtta ccccgattcg ccgtaccgtt
    17941 actattgaag atgtgggtaa ctccgcggca ttcctgtgct ccgatctctc tgccggtatc
    18001 tccggtgaag tggtccacgt tgacggcggt ttcagcatcg ctgcaatgaa cgaactcgaa
    18061 ctgaaataat cgttctgttg gtaaatattg ggcggcgttc tgccgcccgt tatctctgtt
    18121 atacctttct gatatttgtt atcgccgatc cgtctttctc cccttcccgc cttgcgtcag
    18181 gataacgatt tcctttacga ccaaggagcg cccatggaac aacgccacat caccggcaaa
    18241 agccactggt atcatgaaac gcaatccagt actgcggagt atgacgttct gcctctggtc
    18301 ccggaagccg caaaggtcag cgatcccttt ctgctcgacg tgatccttga tgaagaaacg
    18361 ctggcaccct tcctttcatg gctggtccct gcgcgcgttc ttgcagtaga gttgttccct
    18421 gaccagctta ccgtgacccg ttcacagacc ttcaccgctt atgaacgctt gtcgacggcc
    18481 ctgacggttg ctcaggtttg cggcgtccaa cggttatgta actactattc ggcgcgactt
    18541 acgccgctcc ccgggcctga ttcctccagg gaaagtaatc atcggttggc acaaatcacg
    18601 caatatgccc gccaactggc tagctcgcct tctattatcg acaaccgatc gcgccagcat
    18661 ctgaatgacg ttggtcttac tgtctgtgac tgtgtaatca ttaaccaaat cattggtttt
    18721 attggctttc aggcgcggac aattgcgaca tttcaggctt atctcgggca tccagtacgc
    18781 tggttacccg gtctggagat acaaaactat gccgacgcgt cactgtttgc tgatgaatca
    18841 atacgctggc gaagcagcta tgaggtggaa aaactccctg aagagcacac aaaaagttca
    18901 actgcagaac tttgccaact ggccgaaaca ctctctctcc accctatttc actttccctt
    18961 ctcgaaaagt tgctaaacag cacacgggtc aatacacagc cggataatca gcttgcggcg
    19021 ttgttatgcg cgcggataaa tggcagtcct gcttgttttg ccacctgtat ggattcatca
    19081 aatgaatata aaaaaatcag cgcccttctg cgcaaaggcg aaaatgaaat taaccaatgg
    19141 gctgaccgtc attctgttga gcgcgctacc gttcaagcga tacaatggct gacccgagca
    19201 cccgatcgct ttagcgccgc ccagttcagc ccattactcg aacacgaaaa atcatcaacg
    19261 cagattatta atctgctggt atggagcggg ctgtgtggct ggataaatcg cttaaaaatc
    19321 gcgttgggtg agacatatta accttgccgc gtcagacaga ttcgcgtaaa actgtcagcc
    19381 gctctaatgg ccaccaaaat agacaattat gtttcaggac aacccgctgc tagcgcagct
    19441 taaacagcaa ctgcattccc agacgccacg cgctgaaggg gtggtaaaag ccacagaaaa
    19501 aggctttggc ttcctggaag tcgacgcgca aaaaagttat ttcattccgc cgccgcagat
    19561 gaaaaaagtc atgcatggcg accgaattat cgcggtgatc cacagtgaaa aagaacgtga
    19621 atccgcagag ccagaagaac tggttgaacc gttcctgact cgtttcgtgg gtaaagttca
    19681 gggcaaaaat gaccgtctgg ccatcgttcc tgatcatcca ctcttaaaag acgccattcc
    19741 ttgccgcgca gcccgtggcc tgaaccacga gtttaaagaa ggcgactggg cggttgccga
    19801 aatgcgccgt catccgctga aaggcgatcg ttctttctat gctgaactga cacaatacat
    19861 cacttttggt gacgatcact ttgtaccgtg gtgggttacc cttgcgcgtc ataacctgga
    19921 aaaagaagca ccagacggcg tcgctaccga aatgctcgat gaaggtctgg ttcgtgaaga
    19981 tctgactgcg ctggattttg tcaccatcga cagtgccagc acagaagata tggatgacgc
    20041 ccttttcgcc aaggcgttac cggatggcaa acttcagctg attgtggcga ttgccgatcc
    20101 aaccgcgtgg attgctgaag gcagcaagct ggacaaagcc gcgaaaattc gcgcattcac
    20161 caactatctg cctggcttca acatccctat gctgcctcgc gagctttctg acgatctctg
    20221 ctcactgcgc gccaatgaag tccgcccggt actggcatgc cgcatgacgc tctccactga
    20281 tggcaccatt gaagataata tcgaattctt tgccgccacc atcgaatcca aagcgaagct
    20341 ggtgtatgac caggtttctg actggctgga aaataccggt gactggcagc ctgaaagcga
    20401 agcaattgcc gaacaagtcc gtttgctagc gcaaatatgc caacgccgcg gcgagtggcg
    20461 tcataaccac gcactggtgt ttaaagatcg cccggattac cgctttattc tcggtgaaaa
    20521 aggtgaagtg ctggatatcg tcgccgagcc tcgtcgcatt gccaaccgta tcgtcgaaga
    20581 agcgatgatt gccgctaaca tttgtgcggc ccgcgtactg cgcgataagc tcggttttgg
    20641 catctataac gtgcatatgg gctttgatcc ggcgaatgcc gacgcgttgg cagcgttgct
    20701 gaaaacgcac ggtctgcatg tcgatgctga agaagtgctc acgctggacg gtttctgcaa
    20761 actgcgtcgt gaactggatg cgcaaccaac tggtttcctc gacagccgca ttcgtcgctt
    20821 ccagtcattt gctgaaatta gcactgaacc cggtcctcac tttggcctcg gtctggaagc
    20881 atacgccacc tggacttcgc cgatccgtaa atatggcgac atgatcaacc accgtctgct
    20941 gaaagcggtt atcaaaggcg aaactgcgac gcgtccacag gatgaaatca ctgtccaaat
    21001 ggccgagcgt cgccgtctca accggatggc agaacgtgat gttggcgact ggttatacgc
    21061 acgcttcctg aaagacaaag ccgggaccga cacccgtttc gcggcggaaa ttgtcgatat
    21121 cagccgtggc ggcatgcgtg ttcgtttggt tgataacggc gctatcgcct ttattccagc
    21181 acctttctta cacgctgtgc gcgatgaact ggtttgcagc caggaaaacg gcactgtaca
    21241 aattaaaggt gaaacggctt acaaagtgac tgacgttatt gacgtcacca ttgccgaagt
    21301 ccgcatggaa acccgcagca ttattgcgcg cccagtcgcg taatctcctt tcacggccca
    21361 ttcctcatgg atgggccgtt tatttccccg ctcaaccttc ctcatatccc ggcagatttt
    21421 taactgactt tcgtttgaaa actggcgttt ttccaaaact ggattacaaa taaatatata
    21481 aaaaaaacaa tgtgtttagc cgccgttaat ttcatgtaag tcatgttgtt ccttttattt
    21541 aactgcggac tccgctgtta accggaggat atgcatcatg aaaaccgtta gggagtccgc
    21601 aacgttgtac aactttctcg gatcgcacaa tccatactgg cggttgacgg aaagcagcga
    21661 tgttttgcgc ttttctacca ccgaaatcac agaacccgat cgtattttgc aactatctgc
    21721 cgaacaggct gctcgcatca gggaaatgac ggtcatcacc tccagcctga tgatgagtct
    21781 gaccgtcgat gaaagcgatc tttctgtgca tctggtagga cgaaaaatca ataaacgaga
    21841 atggggaggc aacgcgtctg catggcatga cacaccggcg gttgctcgtg atttatcaca
    21901 tgggctttcc tttgctgagc aggtagtttc tgaagcacat tccgcaatag tgattctcga
    21961 cagccggggg aatatccagc gcttcaatcg gttatgtgaa gattacacag ggttgaaaga
    22021 acacgacgtc attgggcaaa gcgtgtttaa actgtttatg agtcgtcgtg aagctgcggc
    22081 atccaggcgc aataaccgtg tattttttcg aagcggcaat gcatatgaag tcgaactatg
    22141 gataccaaca cgtaaaggcc agcggctgtt tctgtttcgc aataaatttg tccacagcgg
    22201 cagtggcaaa aacgagattt ttttaatctg ttccggcacc gacattaccg aagagcgccg
    22261 cgctcaggag cgactgcgta ttctggcaaa taccgacagt atcaccggac tgccgaatcg
    22321 taacgcgatg caggagttaa tcgatcacgc tattaatcag gcagataaca ataaagttgg
    22381 ggttgtgtat cttgatttgg ataattttaa aaaggtcaac gacgcctatg ggcatttgtt
    22441 tggtgaccag ttattacgcg acgtgtcatt ggctatttta agctgtctcg aacatgacca
    22501 ggtattggcg cgtccaggtg gcgatgagtt tctggtactg gcatccaata cctcacaaag
    22561 cgcgctggaa gcattggcat cacgaatttt gacccgctta cgactcccct ttcgcattgg
    22621 tttaattgaa gtttatacca gctgttcagt aggtatttca ctctctcccg aacatggttc
    22681 ggacagcgcg gctattattc gtcacgccga cacagcaatg tacacagcga aggaaggcgg
    22741 acgaggacaa ttttgcgttt ttaccccaga aatgaatcag cgggtatttg aatatctctg
    22801 gctggatacc aacttgcgta aagcactgga aaacgatcag ttggttattc actatcaacc
    22861 gaaaatcacc tggcgtggcg aagtgcgcag tctggaagca ctagtacgtt ggcagtcacc
    22921 tgaacgtggg ttgattccac cgttggactt catttcctac gccgaagaat cagggctaat
    22981 tgtgccttta ggccgttggg tgattctcga tgtcgtacgc caggtagcga agtggcggga
    23041 taaaggcatt aacctgcgag tggcggtaaa tatttctgca cgtcagctcg ccgatcaaac
    23101 cattttcacc gccctgaaac aggttctcca ggaactcaat tttgaatact gccctataga
    23161 tgttgaactg acggagagct gcctgattga gaatgatgaa ctggcactgt ctgttattca
    23221 acaatttagc cgattaggtg cgcaagttca tctggacgat tttggtaccg gctactcttc
    23281 actttcgcaa ctggcgcgct ttccgatcga tgccatcaaa cttgaccagg tttttgttcg
    23341 ggatattcac aaacagcctg tctcgcaatc actggtccgg gcgatcgtcg ctgtggccca
    23401 ggcattgaat cttcaggtga tcgccgaagg tgtagaaagt gcaaaggaag atgctttttt
    23461 aaccaagaac gggatcaatg agcgacaagg atttttgttt gccaaaccga tgcccgccgt
    23521 tgccttcgaa cgctggtata aacgctatct gaagcgcgca taagtagatg aatatatata
    23581 cttagcacta tattccgtca ttatatatcg tattcccgga tgataattag ccgttgctgt
    23641 taaacacttc tcttttagcg gcaatttttg cattattcat tttccccctt ttgctataag
    23701 gatgtccttg atgtctgaat ttgatgccca gcgcgttgcc gaacgtattg atattgtgtt
    23761 agatatcctt gtggcaggcg attaccactc tgcgatccat aatctcgaga tacttaaagc
    23821 agaactcttg cgccaggttg ctgaatctac gccagacatc ccgaaggcac catgggagat
    23881 ctaacaatcg tgccttcagg ttttcctatt tgaggatgcc cggcgcgtca gtattttttt
    23941 atttagtatt ataacgttat aagaattaca gcgatgaatt cccgacaaca aactattcta
    24001 cagatggtca ttgaccaggg tcaggttagc gtaaccgatc tggcaaaagc cactggagtt
    24061 tctgaagtta ccattcgcca ggatctcaac accctcgaaa aactgagtta cctccgacgt
    24121 gcacatggtt ttgcagtttc gcttgatagt gatgacgtcg aaacccgtat gatgagcaac
    24181 tatacgctga agcgtgaact cgccgagttt gccgcgtcac tggttcaacc gggcgaaacc
    24241 atctttatcg aaaatggtag cagcaatgcc ctgctcgcca ggactctggg cgagcagaag
    24301 aaaaatgtca ctatcatcac ggtcagcagc tacatcgcgc atttgctgaa agacgcgcct
    24361 tgtgaagtca ttttgctcgg tggcgtgtac cagaaaaaaa gcgaaagtat ggttggccct
    24421 ttgacacgcc agtgcatcca acaagtgcat ttcagcaaag catttattgg tattgatggc
    24481 tggcaacctg aaactgggtt taccggtcgc gatatgatgc gtaccgatgt ggtcaatgcc
    24541 gtgctggaaa aagagtgcga ggcgatagtc ctgactgaca gctcgaaatt tggtgctgta
    24601 cattcatact ccatcggccc cgttgagcga ttcaatcgcg tgattaccga ttcgaaaata
    24661 cgcgccagcg atctgatgca tcttgaacaa agtaaactca ccgttcacgt cgttgacatt
    24721 taaccatttc ccttctctac ggatgatttg cagtttggca aatcatccgc tctaagatga
    24781 ttcctggttg ataattaaga ctatttacct gttattaaca ctctcaagat ataaaattat
    24841 tatcagcgat ataacaggaa gtcattatca cctgcgtgat ataaccctgc gcgcgagcgg
    24901 atttcacgga ataatttcac caggcttatt cttagctatt atagttatag tgagcttact
    24961 tccgtgaatc ataaattcag gagagagtat tatgtttgta acgagcaaaa aaatgaccgc
    25021 ggctgttctg gcaattaccc tggcaatgtc tctgagtgcc tgttctaact ggtctaaacg
    25081 agaccgtaac accgcaattg gcgcgggtgc aggtgcatta ggcggtgcag tactgaccga
    25141 tggcagtacg ctgggtacat taggtggtgc tgccgtcggt ggtgttattg gtcaccaggt
    25201 cggtaaataa tcgtaaataa atgtcgatta tcgactcgca attataatac gcttgattct
    25261 gttcagtacg aaataactga ataatacaca cagtaaaata ttcgaaagcc acggatatat
    25321 ccgtggcttt ttatgttaac cgcctgcgag ttttaccttc atccctttcg cttcaagcag
    25381 tgattttaat aaatcacgct tatcgccctg gatttcaata attccatctt taactgctcc
    25441 gccgcagccg cattttttct tcagttccgc tgccagtttc gtcagttcgg catcatcgag
    25501 agcgacaccg gtaatcaggc aaacgccctt acctttacgt ccactggtct gacgctgaat
    25561 acgcaccaca ccgtcgcctt tagggcgtac aggggccgct tttggctcat caatacgtcc
    25621 ggtttccgtt gagtagacca gacggctgtt ggaatcactc atcatgcact ccgctgtaaa
    25681 gaggcgttga tcgctttcag cgtctgcgct ggatctaccg attgcgttac cgggcgacca
    25741 atcaccatat aatcaacacc agccgccaac gcctgttctg gcgtcataat gcggcgctgg
    25801 tcaccagcat cactcccctg cggacgaatg cccggcgtaa ccagtttgaa ctcctgaccg
    25861 aatacctgtt taaagcgcac agcttcctga gcagaacaca ccacaccatc aaggccacat
    25921 ttttgcgtca gtgccgccag acgttctgca taatctgcag gtgacagtgt cacgccaaga
    25981 tcggccaggt cgctggcttc catgctggtc aacactgtca cagcaatcaa aagcggtgca
    26041 tctttgccaa acggaaccag tgcctcacgc gctgcggtca tcatacgcgc cccaccggag
    26101 gcatgaacat tcaccatcca cacgcctaag tcagccgcag cagcgacagc gtgcgctgca
    26161 gtgttgggaa tatcgtggaa tttcaggtca agaaagatat caaaaccacg ctgttgaagt
    26221 tcgcgcacaa actgtggccc aaacaatgta aacatctctt tgccgacctt cagacgacaa
    26281 tcgcgtgggt cgatcttgtc gacaaaggcc atcgcggcat cgcgattatg ataatcaagg
    26341 gcaacaacta caggagaatt cgtaacagcg cgggaagaag atgaagcagt taacgtcatg
    26401 accagacctt cttgatgatg ggcaccggaa gtggcgcgaa acaggtaaac ggcgagcatt
    26461 ctacccgtgg aaataaaaaa ttaacagttg cgatttctcc tctggcaggc ttttccgcgc
    26521 cgtcggtgcc ggagcacaat agaacatcaa taattagtat gttgtaacta aagtcggatt
    26581 tttttaaaaa ttacaggcca tcaagaccgc gaattggttt aatggttgac caggcccgac
    26641 aagacggaca atgccagtag agggtgtatg cggtaaaacc acatttctgg cagcgataac
    26701 ggggcttact acgtaccttc tcgccaacca tgtcacgcag caccatcaga ctctctttgg
    26761 cacgcccttc ttctgcttca tttaagtggt aatccattaa cttatggaac acacgcatgg
    26821 tcggatgacg ctgaagctgg cgagtaatat agacctgtgc ggcctcacta ccgtcgcgcg
    26881 cttcgatgat atccgcaagc atcaattcag catcggcacc ggtgttctct tccaccgcgc
    26941 gctgcaggaa ttctgcccat tcggcagttt tacccaactg ctgatagcat gtttgcagca
    27001 tttccagcgt ttcgctgacc agttctctgt cctgggatat gacacgttgc aaactttcga
    27061 cggctttggc gtattctcct ttcgccataa acacgcgtcc catcatgatg gatacgcggg
    27121 cgctgttttt atctgccgcc gcaccttttt ttagcaaagt catggcacga tcgagatcgt
    27181 cgctggccat atgctgcaga gctaactcac agtagaaatg ggcaatttcg acgcgctgtt
    27241 tatctttacc cagcttcacc aggcgttcgg caacatcaat tgctttctgc cactcgctgg
    27301 tagcctggta aatttgtagc agttgttgca gcgcgccaat gcggaagtca gtttcatctg
    27361 tcagctgatt gaacatgtct tccgcgcggt cgtataaccc ggcagccatg taatcacgcc
    27421 ccaattgttg aatcgccaaa agccgctgtt cataggtcag cgaggcgctt tccattaggg
    27481 tctgatggat gcgaatagcg cgatcaactt cgccacgcga acggaacagg tttccgagcg
    27541 taaggtgggc ttcaacggta cctgtatcct ctttaagcat atcgagaaac aggtctaccg
    27601 ctttatcctg ttgattacta agcaggaagt taacccccgc tacgtaatca cgcgacaagc
    27661 ggttggcttc atcttgcttg ttttgttgcg cacttctgcg gcccatatac cagccatagg
    27721 cagcggctac aggcaaaagc agaaacaaca actccagcat agaaagttat tccttcgacg
    27781 cagacgagtg cggcgctaca gccacgtcag tcgcgggtga aagctggttt tccagtcgct
    27841 ttattttacg ttcagcgcgc gccagggaaa cacgaactcg cagccagaac aggccacaaa
    27901 tcaaccaacc gatagcaaac cccgcagcaa acaataccgc cagcaatgtg gaaatacggt
    27961 actccccttg cgctaacaga taattaaacg tcacctgttg atcgttctgc gcacccaacg
    28021 tgaccgaaat cacgaagatc gctaacacca gtaagaaaat gagtaaatat ttcacattac
    28081 ttcccgttat gtggttagag cgaataaatt atggtcaact taccgcaata gccctttaaa
    28141 gtaccatttt cgcgccgtct gcgaaatggc atccacgcgc attgttgatg atttaagggc
    28201 taaattcgga aaatcaacca acgttaactt tcttgttctc gttgtgcaat ttcgcgattt
    28261 tcttccgcag gtggtgttaa tggcccacaa attcgttgcg caagccacgt tgccaccgcc
    28321 accagcgccc acgaaatcaa cgtagctaca accagatcgc gtggccaatg catcccgagc
    28381 agcaggcggc ttcccatgac tcccgttgcc cagaccaaca agatagcaat ggttaacgtt
    28441 cgccgacgcg gccacagcaa accaacggcc agcagtgccc aactggcagc aaacatcgtg
    28501 tgaccggaag gaaaggcaaa ccccgtctct ttctgccagt gtgaacgcaa atattgtggg
    28561 atatttttct cttcagccaa ctgttctttc accagatttc cgcgttctgc tcgctttaaa
    28621 gtgtagaact catcaaccgg aatatgatgt gttttttcca gccagataac aaaaggtcgt
    28681 ggttcctgga ctttgtcttt gatccaggat ttaacgcctt gtcccataag gattgccgcc
    28741 gccagaatgg caaataacat aatggcagcc ttaatgcgaa aacgcagaca ccagagaaac
    28801 cagccgaata aaatcaaatg tgtaatgacg ccccagggct gggtgacagt ttcagtaacc
    28861 caaaaagccg cttttagtaa ccaactttgt tctccaggtt gccaacgcca gccagaaagc
    28921 catacggcta ctggcatgac aagcaatagt gcagctccca ctgcggtacg tctggcaatc
    28981 gaacgcatgg cctctccttt ttgataagtc ccacaatcat aactgaaaat gccagttcca
    29041 ggaaaaattg acagatttgt gccattccgt gaacgatcga cgcgtcgtga ttaggtgaac
    29101 cccttctcgt tatggcaaaa taagccaata cagaaccagc attatctgga gaatttcatg
    29161 cagcttaaac gtgtggcaga agccaaactg ccaaccccat ggggcgattt cctgatggtg
    29221 ggatttgaag aactggcaac cggacacgat catgtcgcgc tagtctatgg cgatatttcc
    29281 gggcataccc cggtacttgc gcgcgtccat tccgaatgtc tgaccggtga cgccctgttc
    29341 agcttgcgct gcgattgtgg cttccagctc gaagcggcat tgacgcaaat tgccgaggaa
    29401 ggccgtggta ttttgctgta tcaccgtcag gaaggtcgta acattggtct gctgaataaa
    29461 atccgcgctt acgcactgca ggatcaaggt tacgatactg tagaggctaa ccaccagtta
    29521 ggcttcgccg ctgatgagcg cgacttcact ctttgcgctg atatgttcaa actccttggc
    29581 gtcaatgaag tccgcttgtt aaccaataac ccgaaaaaag tcgaaattct gaccgaagca
    29641 gggattaata ttattgaacg cgtaccgttg attgtaggtc gtaaccccaa taacgaacat
    29701 tatctcgata ccaaagccga gaaaatgggt catttgctga acaaataacc ctcttgcatt
    29761 gtgtaattga tttgcttgcc ggaagcaaaa taaccggcaa gcaaatactc gttacttcaa
    29821 catattacga atgacataat gcaaaatgcc gtcgttctgg tagtaggtca actccgtcgt
    29881 ggtgtcgata cgacaacggc agggtacgac ttcctggcta ccatccgcgc gcgtaagcgt
    29941 caccggaacc gtcgcgccgg gttgtagatt ttgcagatca ccaatatcaa tcttctcttc
    30001 cccggtaagc tctaacgttt tacgcgtcac accttgtgga aattccagcg gcaggatgcc
    30061 catgccaatt aaattcgaac ggtgaattcg ttcaaacgat tcggcaatca caacacgaat
    30121 accaagcaga cgcggacctt ttgccgccca gtcacggctg gagcctgatc catactcttt
    30181 cccggcaatc accgccagcg gcgtttgctc ctgcttatag cgcattgcgg catcataaat
    30241 agagacgaca tcgctgtcag gtaaatggcg cgtcatcccc ccttcaacgc caggcaccat
    30301 ttcattacgg atgcgaatat tggcgaaggt gccgcgcatc atcacttcat ggttaccacg
    30361 ccgcgaacca taggagttaa agtctttccg ctcaacaccc cgaccttgta gatatcgacc
    30421 cgccgggctg tcgggcttaa tactgcccgc cggagagata tggtcagtgg tgactgaatc
    30481 ccccagcatt gcgaggatcc gcgcaccgtg aatatcttcc acaggtgctg gtgttgcctg
    30541 catttcatca aagaaaggcg ataagcgaat ataggttgaa tcctcctgcc aaccgtaggt
    30601 atcggatcgt gtgacgttaa tttccttcca ctcggctgtg ccttcaaaaa cttctgcgta
    30661 ctctttgcgg aacatttctg tggagacttg gtctaccgca cgggcaattt cttgtgccga
    30721 tggccagata tctttcagat ataccggctc gcctttgcga tcatggccga taggctcaga
    30781 agccaggttg atattcatat ttcccgccag cgcataggca accaccagcg gcggcgaggc
    30841 cagccagttg gttttaacca gcggatggat acggccttca aagttacggt tgccggacag
    30901 cactgcaccg acggttaaat cgcctttttt gattgccgtt tcgataggat cgggcaacgg
    30961 tccagagtta ccaatacagg tggtacaacc gtatcccaca aggttaaatc ccagttcatc
    31021 gagatacggt gtcagtttcg cttttgccag ataatcagaa acgactttcg aacccggtgc
    31081 cagcgacgct ttgacccacg gttgccgctt gaggcccaga gttacggctt ttttcgccag
    31141 caagcctgcg gccatcagca cacttgggtt agaggtgttg gtgcacgacg ttatcgcagc
    31201 aatgaccaca gcgccatcag gtaactgata ctgatgtccg ttcataacat aatcgaccgg
    31261 caggcgatct ttatgcgtgg cattcacttc cagttcgcta ctggcagcaa atgcttttgg
    31321 tacatcgggc agtgcaacgc gatcctgcgg gcgttttggc cctgcaaggc tcgcttcaac
    31381 gtcattcata tccagttcta acacactggt aaaaattggt tcatcgcccg gattacgcca
    31441 catgccctgc gctttggcat atttttcgac caactcgatc tgatcttcac tgcgcccgct
    31501 taaacgcatg taatcgaggg tgacagcatc gattgggaag aagccacagg tggcaccata
    31561 ttctggcgac atattggcaa tggtggcgcg atccgccagc ggtagtgaat ccagcccatc
    31621 accataaaat tcgacgaatt tccccaccac gccatgtttg cgcaacattt gggtaacagt
    31681 gagaaccagg tctgtggcgg taataccttc acgtaatttt ccggtaagtt taaagcccac
    31741 tacgtccggg ataagcatgg aaaccggctg gcctaacatt gcggcttctg cttcgatgcc
    31801 accaacgccc caccccagca cgccaaggcc gttgatcatg gtggtgtgcg agtcagtacc
    31861 aacgagtgta tccggataag caatccattc accgtcctgc aattcgctcc acactgcttt
    31921 gccgagatat tcgaggttaa cctgatggca aatgcctgtg cctggcggca cgacgctaaa
    31981 ccgactgaac gcctgctttc cccatttcag gaacacataa cgttcgtggt tgcgctccat
    32041 ttccaggcgt acgttttctt caaatgcctc atcatcacca aaacgatcga cggtcaccga
    32101 gtggtcaatg accaggtcga ccggtgagag tgggttaacc tttgcagtat cgccgccgag
    32161 gcgtttaacc gcttcgcgca ttgccgccag atcaacaacg gcaggtacgc cggtaaagtc
    32221 ctgcatcagc actcttgccg ggcggtaggc aatttcacgg tcagcatggg catttttcag
    32281 ccatcccgcc agcgcgtgga tatcctcttc ggtaaccgat ttaccatcct gccaacgcag
    32341 caggttttcg agcaaaactt tgagtgactt gggtagacgg gtgatatcgc ccagtgattt
    32401 ggcagcaagc ggcaagctgt agtagtggta agttttatct ttggcctgca acgtgtcctt
    32461 actggcttct cgtagggttg acgacatagc tcctccttaa tgacagggtt gcgaagccct
    32521 gattctcttc agggtgaata ttaaagataa cacaaacatc gcgtaacgat ttgataacaa
    32581 cccaaattga taaaagagga gaaaccttga ggcaaagcaa acagcggggt cagggatcag
    32641 tgagtaatga tccagaggag ttcggcacag aagagcatcg acagagaaaa tgcgcccatc
    32701 caggaccaat atttcatatt tgtgttgatc ataggctaca cccttaacgg ttattacttt
    32761 gtgttgagat aactcaacct gttattgctg cggtgtatta aaagccctgt aattcattaa
    32821 aaatgggtaa taagaatgat gataattatt gtttcggctg atcatcttca gcaaacagtt
    32881 ctataaaggc ttgttgctgc gatgttagtt gccagtcgtc tggaataacg acggaacatt
    32941 cccgttttgc ttctggttca ttgtcactat gctgacataa tggtgacgac tccagttgct
    33001 cctgaccgac catagttatc cccacaattt ccaaataaac agtgcgacaa ccacccagaa
    33061 aagtgcagaa acaagaaaaa ccgccagcca ggctttacgt tttaattcag gattgcggcg
    33121 gggttcctga tttccagaag gcattgctaa cctcatataa tcgccatcac ttatcagcaa
    33181 gacgttgaac gattggtgta attaatcata cgatgagaca aatcctaaag aaactttagt
    33241 caaaaatcca gcgaatttac gaatcgtctc cagagaataa ttcaa
//
LOCUS       DAZFFE010000043        32968 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZFFE010000043 DAZFFE010000000
VERSION     DAZFFE010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.043"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(81..1355)
                     /gene="fadK"
                     /locus_tag="ACHJ69_002234"
                     /pseudo
     CDS             complement(81..1355)
                     /gene="fadK"
                     /locus_tag="ACHJ69_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(1412..1705)
                     /gene="ydiT"
                     /locus_tag="ACHJ69_002235"
     CDS             complement(1412..1705)
                     /gene="ydiT"
                     /locus_tag="ACHJ69_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO5015217.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIEFRYG"
     gene            complement(1702..2991)
                     /gene="ydiS"
                     /locus_tag="ACHJ69_002236"
     CDS             complement(1702..2991)
                     /gene="ydiS"
                     /locus_tag="ACHJ69_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5015218.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPIERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGTQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVSASDPHHYAVGVKEVIGLTPEQINDRFNVTGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLINGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVHGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNDMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(3046..3984)
                     /gene="ydiR"
                     /locus_tag="ACHJ69_002237"
     CDS             complement(3046..3984)
                     /gene="ydiR"
                     /locus_tag="ACHJ69_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO5015219.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTEQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDISH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLEISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(4004..4768)
                     /gene="ydiQ"
                     /locus_tag="ACHJ69_002238"
     CDS             complement(4004..4768)
                     /gene="ydiQ"
                     /locus_tag="ACHJ69_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO5015220.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLNTAKALA
                     TAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            5084..5995
                     /gene="ydiP"
                     /locus_tag="ACHJ69_002239"
     CDS             5084..5995
                     /gene="ydiP"
                     /locus_tag="ACHJ69_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5015221.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(6038..7189)
                     /gene="ydiO"
                     /locus_tag="ACHJ69_002240"
     CDS             complement(6038..7189)
                     /gene="ydiO"
                     /locus_tag="ACHJ69_002240"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO5015222.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(7203..8798)
                     /gene="ydiF"
                     /locus_tag="ACHJ69_002241"
     CDS             complement(7203..8798)
                     /gene="ydiF"
                     /locus_tag="ACHJ69_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO5015223.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPCIISDIGIGTFVDPRQQGGKLNEVTKE
                     ELIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(8944..9702)
                     /gene="aroD"
                     /locus_tag="ACHJ69_002242"
     CDS             complement(8944..9702)
                     /gene="aroD"
                     /locus_tag="ACHJ69_002242"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO5015224.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVIAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(9733..10599)
                     /gene="ydiB"
                     /locus_tag="ACHJ69_002243"
     CDS             complement(9733..10599)
                     /gene="ydiB"
                     /locus_tag="ACHJ69_002243"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO5015225.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTDKDFP
                     LEYVKQVMGFGA"
     gene            complement(10611..11876)
                     /gene="ydiN"
                     /locus_tag="ACHJ69_002244"
     CDS             complement(10611..11876)
                     /gene="ydiN"
                     /locus_tag="ACHJ69_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5015226.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGEHKFSTRLNTIKHRG"
     gene            complement(12103..13317)
                     /gene="ydiM"
                     /locus_tag="ACHJ69_002245"
     CDS             complement(12103..13317)
                     /gene="ydiM"
                     /locus_tag="ACHJ69_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5015227.1"
                     /translation="MKNPYYPTALGLYFNYLVHGIGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRHLPVIKKTTSSTEHRCSIIDLASYSLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALLTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFILALFIGLRSRKETR
                     HHTLKENIAPGG"
     gene            complement(13417..13773)
                     /gene="ydiL"
                     /locus_tag="ACHJ69_002246"
     CDS             complement(13417..13773)
                     /gene="ydiL"
                     /locus_tag="ACHJ69_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO5015228.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGNSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(13909..14016)
                     /gene="rprA"
                     /locus_tag="ACHJ69_002247"
     ncRNA           complement(13909..14016)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHJ69_002247"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(14202..15314)
                     /gene="ydiK"
                     /locus_tag="ACHJ69_002248"
     CDS             complement(14202..15314)
                     /gene="ydiK"
                     /locus_tag="ACHJ69_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO5015229.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKSNK"
     gene            15528..18584
                     /gene="ydiJ"
                     /locus_tag="ACHJ69_002249"
     CDS             15528..18584
                     /gene="ydiJ"
                     /locus_tag="ACHJ69_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO5015230.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDETLIDERVNALCARLDELIASQQAGVIGWQVCSEL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            18581..18991
                     /gene="menI"
                     /locus_tag="ACHJ69_002250"
     CDS             18581..18991
                     /gene="menI"
                     /locus_tag="ACHJ69_002250"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO5015231.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVHSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            19091..19279
                     /gene="ydiH"
                     /locus_tag="ACHJ69_002251"
     CDS             19091..19279
                     /gene="ydiH"
                     /locus_tag="ACHJ69_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO5015232.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            19828..20196
                     /gene="sufA"
                     /locus_tag="ACHJ69_002252"
     CDS             19828..20196
                     /gene="sufA"
                     /locus_tag="ACHJ69_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO5015233.1"
                     /translation="MDMPSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMIGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            20205..21692
                     /gene="sufB"
                     /locus_tag="ACHJ69_002253"
     CDS             20205..21692
                     /gene="sufB"
                     /locus_tag="ACHJ69_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO5015234.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYHAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDN
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYRKKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            21702..22448
                     /gene="sufC"
                     /locus_tag="ACHJ69_002254"
     CDS             21702..22448
                     /gene="sufC"
                     /locus_tag="ACHJ69_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO5015235.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTSGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            22423..23694
                     /gene="sufD"
                     /locus_tag="ACHJ69_002255"
     CDS             22423..23694
                     /gene="sufD"
                     /locus_tag="ACHJ69_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO5015236.1"
                     /translation="MAGLPNSSNALQQWNHLFEAEGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLDGLTNSQFVSIAGEIAPQQRDALSLTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTVRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALCDEGLKQQVLARIGQRLPGGAR"
     gene            23691..24911
                     /gene="sufS"
                     /locus_tag="ACHJ69_002256"
     CDS             23691..24911
                     /gene="sufS"
                     /locus_tag="ACHJ69_002256"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO5015237.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            24924..25340
                     /gene="sufE"
                     /locus_tag="ACHJ69_002257"
     CDS             24924..25340
                     /gene="sufE"
                     /locus_tag="ACHJ69_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO5015238.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDKSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            25489..26493
                     /gene="ldtE"
                     /locus_tag="ACHJ69_002258"
     CDS             25489..26493
                     /gene="ldtE"
                     /locus_tag="ACHJ69_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO5015239.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYNYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(26556..26792)
                     /gene="lpp"
                     /locus_tag="ACHJ69_002259"
     CDS             complement(26556..26792)
                     /gene="lpp"
                     /locus_tag="ACHJ69_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO5015240.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(27103..28515)
                     /gene="pykF"
                     /locus_tag="ACHJ69_002260"
     CDS             complement(27103..28515)
                     /gene="pykF"
                     /locus_tag="ACHJ69_002260"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO5015241.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQSFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(28523..28717)
                     /locus_tag="ACHJ69_002261"
     CDS             complement(28523..28717)
                     /locus_tag="ACHJ69_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015242.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHNHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            29073..29282
                     /gene="fumD"
                     /locus_tag="ACHJ69_002262"
     CDS             29073..29282
                     /gene="fumD"
                     /locus_tag="ACHJ69_002262"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO5015243.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            29738..30364
                     /gene="ydhY"
                     /locus_tag="ACHJ69_002263"
     CDS             29738..30364
                     /gene="ydhY"
                     /locus_tag="ACHJ69_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO5015244.1"
                     /translation="MNPVDRPLLNIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            30385..32487
                     /gene="ydhV"
                     /locus_tag="ACHJ69_002264"
     CDS             30385..32487
                     /gene="ydhV"
                     /locus_tag="ACHJ69_002264"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO5015245.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEDMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            32500..32922
                     /locus_tag="ACHJ69_002265"
                     /pseudo
     CDS             32500..32922
                     /locus_tag="ACHJ69_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
BASE COUNT         8535 a         7911 c         8306 g         8216 t
ORIGIN      
        1 gacagctgat agaaacagaa gccactggag cacctcaaaa acaccatcat acactaaatc
       61 agtaagttgg cagcatcacc gccagcctca tccatgcggc agagatcgcc gctgtaatac
      121 cagccttctt catccagcgc acgggcggtt aattcaggtt catcaaaata ccccataaac
      181 acattggggc cacgcgaggc ttcttcacct tcgcaacctg gcggtaaagt cttgcgtgcg
      241 tcatcgacca ctttaatctc tacacctgcg gcagcgtaac cgtcggtgtg cataaagcgc
      301 ggcaaaggat cgtcgagatt caccaccgca tgcggcgaac tttctgtgga accataaaca
      361 cttaataatt taatgccgcg ctgctggcat tctcgcgcca cttttttggg gattgtggta
      421 cctccgcaaa gaaagaagcg cagcgctgaa agatctgcgg gttgtttctc tagtaaattc
      481 aaaagatcat agacaaacgg cgttgcgccg agcatacagg tgcaacgctg ctgctcaagc
      541 agcgcgagac acgcatcagg agtgaaaata tctaacaaca cgctgcgagc accaatcaaa
      601 aaaggtgctg ttacgccatg cagaaagccc gttgcgtgac caagtggcgc aggcatcata
      661 aagacatcct gccaggtcag atttagccgc gcgcaataag cccgctcact ggcgagaata
      721 ttgttatgcg ttagcatcac accctttggc agaccctcgg ttccggaggt aaatagcaca
      781 gcggctaatt catcggcgtg ggtcgttatc gccgtggtca gaggagtatt gtcggcgaga
      841 atctgactta atgagaggga agaggtggcg ggagtcagtt tgtccacgcc gacaatttgt
      901 tgtagttgtg gaagctgatt ttgcagcggc aggattaaat ctaccggacg cgtttgttta
      961 aacaacgtcg gtgcaaagaa catttttgcc tgacacttat tgagcaccca caccagttct
     1021 gcttcccgcc aggaaggtaa cagcggcacg gaaaccgcac cggttttcag gcaggcaaga
     1081 tagataacgg taaattcaca ccagcctggc agttgaaatg cgatgcgatc acctgattca
     1141 atacccttcg ctaacatcca gtttgccaga cagctcgcgg cgtgatcgag cgcgctatag
     1201 gtgtacgttg caccatgatt gtcgaccacg gcaattttgt ctggcatcgc acgagcggtc
     1261 tgctgccagt aatctgccag tgaagcatcg ccccataacc cttgctgacg atacgccgca
     1321 cgacgttgtt cgttgaacgt taatgtcact ttcattttgg actctcgaaa cagaacatca
     1381 ggcccgagat gcgggcctgt gggatgcatg attagccgta gcgaaattca ataccgaagg
     1441 tgcctgcggg atattgccac tgttcgagaa tagtgttgcc gcacagcacc cgacaggtgc
     1501 cgcactccag acaaccggcg gaatcgaaat gaatgtttcc tgcgtcatcc tgcttataaa
     1561 gtccggcagg gcaagctttc attaatttac ggaattcatt gatatcggga tttgccgcca
     1621 aaatgatatg cgggtggccc tcatcaacat ggaatttatt gacgcctaat ttgatgtcga
     1681 cgttaaccgt agcgttctgg ctcatagcgc ggttgctccc ttaatgccat ctttcagcaa
     1741 gttgatcagc ccaattttct tcgcgtgtcc catgatcatt ttgcgtaccg gctggtttgg
     1801 tttgccgtca atggtgaaca tatcgttcat gatgtcggcg accattcgtg ggtattggct
     1861 aaacaggcgc gggttttcca tcagcgccgg gatcttgcgg aaatgctgca tatcgcgcat
     1921 aacacaactt tgttccagct cacgtttgta ttgcgccaga ctgctggcgg agaaatccgc
     1981 gcgttctttg gcggcgatca ccgttgtggc ggcagcctga gctgatgcaa tggctaaatc
     2041 catgccgtga actgtaaaac ccaaattcag gcagaagcct gcggcgtcac caacgatcat
     2101 cacgccatcg ttaaccagct gcggcaccat tgccagaccg ccctccggca ccatatgcgc
     2161 ggaatattca agcagtttgc cgccgttaat cagcgggcga atggcggggt gttgtttaaa
     2221 atcttccagc atttgcggca cgcttttttg cgcatgggcg atatcaccca atccacaaac
     2281 cagccccaac gatacggaat ctttgttggt atagaggaat ccgccgccca tcaggccgtc
     2341 agaaggggaa ccggcaaaca gccaggcggc accttcctcg cccgtaacat taaagcgatc
     2401 gttgatctgt tctggtgtca ggcctataac ctctttaaca ccaacagcgt aatgatgcgg
     2461 atcggaagcg gaaaccattc ccagcgagcg gccaagcatc gagttaacgc catcagccag
     2521 aatcaccaca ttcgcttcga gaatatcatc cccggcctgg acgccagtga ccttgtttcc
     2581 ttcacgaacc agcgcatcga cgcgtacgcc cgggataaac tgcgtgcccg cctgctcggc
     2641 ttgttccatc aaccacgggt ccagacgatt acgcaatacg gtataagacg cgtgttgcgg
     2701 aacatctggt tgctcgcggt gaaaatcgag ggtaacggcg ctttcttcgg ttaagaagga
     2761 gattttctcg cgtgtgacct tgcgttctat cggcgctgat gctgcaaagc ctggaataat
     2821 tgcttcgagt gtgtgggcat aaagacgccc gccggtcatg tttttacatc cggcactgtc
     2881 gccgcgttct atcaccagga catccagccc ggctcgcgcc atgaccagtg cggcaacgct
     2941 accggcaacg cccgcaccga ccacaatggc atcaaatttg tcatccgaca tgcgttatgc
     3001 tcccttcccg ctgcgtttac agcgggagat gtggttgaaa gggaattagc gactcaactg
     3061 gctaatcagg gcagggacga ctttgtagat atcgcccacc agaccgtagt cggcatagtt
     3121 gaagattggc gcatttttat ctttattaat ggcgacaatc acttttgcgc cgttgccgcc
     3181 aaccatatgc tggatctgcc cggagatctc cagcgtcagg tagagatcgg atttcagcaa
     3241 cacgccggag acaccgatat aacgttcacg ctccatccag ttctcgcctt cggcaattgg
     3301 acgtgaacag ccgacttcag cattcagcac cgccgccagt tcgtggacca ttttcaggtc
     3361 atcctgcgcc gccagaccac gaccgacgcc aaccacacgt tttgctttgc tcaggtccac
     3421 gctgcttgcc gctttcgcac ggcgttcgcg acagagaatt tcatgacgcg gggcaacata
     3481 aggtaccgtt tctgtcgggc actgatgaga gatatcactg gtgcacggct cctgaacacc
     3541 gggtgcaagg gtaatgatcg ccagcgggct gttgatcttt tcctgagcga acgctaaccc
     3601 gccatacatc cggtgttcgg cgcaaatgtg accatcgaca atatccacag ccgtggcatc
     3661 gttcaccagc gccgcattca gttgcacact taaccgtgct gccagagctt taccacgttt
     3721 cgtcgcggcc aacagcaaca tagcggggtg tttatctttc agcagggcag caatgctttc
     3781 ggcgtaattt tcagtacgtt gcagcgcgtc gttttgctca agaacataaa tgcattttgg
     3841 cccataaggc ataactgcct gcgcctgctc ggtattttgt acaatggcat acacctgttg
     3901 gccccattgc tgagcgccgc caaacagttc agcataacgt tcaggattat cgctaaagac
     3961 ccagacgctg tttaattgac tcatcgtttc ttctccgtag gcttcagttc agggctttct
     4021 tcagatgttc cgccagctcg gcaatggcct ccggcgaatc gttatcgaga atgatgtgct
     4081 tacgttctgt ttgcggcggt acgcgaatgc cggtcagttc agcaagtggt gcgctctggc
     4141 tccagccaat atcacttgcc tgccactgat ttaccggttt tttacccgcg ccgagaatgg
     4201 ctttcatcga aggaatacgt ggcacgttaa tatcggaggt gacgcagagc acggctggaa
     4261 cagagagttc aataacttca acatcatctt caagcgtgcg ttcaatcacc agtgtattgc
     4321 cctgacgctg aatggcactc actgcattaa tcaccggaag ttgcagaatt tctccgacca
     4381 gcaagccaac ctgctgggca taaaggtcgc cggaaccttc accaaagatc agtaaatcga
     4441 agccgatctt ttcaactgct gtcgccagcg cctttgcggt attgagaggc agtgcatgct
     4501 caagttgtgc atcctgcacc atatacaggc tgtgcggccc gcgggatagc acgtctttgc
     4561 gcactttcga gttctgcaac aaagagccgc cgacggtcag cgcggctatc tcatcgtcat
     4621 ctgttgcaag ctggcttgca gcttcaatgg cattgagatc gaactggctg attttggcgt
     4681 cggcattgtc gaaattcagg gtgtattctg gagtgacaac aatgtcctgt tcttcaggca
     4741 ccagcttaaa gcaggttatt attttcatga tttctcctgt gagtcgcata cggaaccatc
     4801 tccgatggaa ggtaaaaatg gcctgaaaat agtgtgcgat agcggtgcgg ggttttcgac
     4861 ggagcaagct gttttccttg ccaacaatct tgcaaaaaaa atgcgatcgc taaaacaaaa
     4921 gtgcaatttg cgggaatata gttattccat gaccgtgcaa tcttgcattc agtgtaaata
     4981 atcttgttta gctctcgcta aacgcggttg tataaccgca atacacgccg tattttatcc
     5041 ggcatatatt gtttttgttt gctcatggaa tagggaggtt gtcatgtatc aacgctgttt
     5101 tgataatgcc agtgaaacgc tgtttgtcgc tggtaaaacg ccacggcttt cacgtttcgc
     5161 atttagcgat gatccaaaat gggagtcagg acatcacgtt catgacaatg aaaccgagct
     5221 gatttacgtc aagaaagggg tagcaaggtt taccatcgat tcttcgttat atgtcgcgca
     5281 tgccgatgac attgtggtga tagaacgtgg ccggctgcat gcggtggcct ctgacgttaa
     5341 cgatccggca acgacgtgta cctgtgcgct gtacggcttt cagtttcagg gggctgagga
     5401 aaatcagcta ctgcaaccgc attcttgccc ggtaattgcc gcagggcagg gaaaagaagt
     5461 cattaaaact ttatttaatg agctaagtgt gattttgccg caaagtaaaa atagccaaac
     5521 atcttcgtta tgggacgcat ttgcctatac gttagcaatt ctttactacg aaaattttaa
     5581 aaatgcttat cgttcggagc aggggtatat taaaaaagat gttctgataa aagatattct
     5641 tttctatctg aataataact atcgcgaaaa aatcacttta gagcagttat cgaaaaaatt
     5701 tcgtgccagc gtcagttata tctgccatga atttaccaaa gagtatcgta tttcccctat
     5761 taactatgtt attcaacggc gtatgacgga agcgaaatgg tcactcacta atactgaatt
     5821 atctcaggca gagatctcct ggcgtgtggg ttatgaaaat gtcgatcact ttgccaaact
     5881 gtttttgcgc catgtcggct gttcgcccag cgattaccgc aggcaattta aaaactgttt
     5941 tgcggaacaa gaaatcctat ctgaatttcc tcaaccggta agtcttgccg gataaatctg
     6001 gcggcgcagt ttgttaagag gctgcgccgc ctgcagatta tttgttctga taatctttca
     6061 ggatctgccg acctgctacg taaatcataa tttcgtctgt accgccgccg atacgttcac
     6121 aacggacatc acgccagaag cgggagacgc gcgcctcatc ggtatagccc agaccgccca
     6181 tgatttgaat cgcatcatca atgacttcca ttgcggtacg tgcgcagtac agttttgcca
     6241 gcgccgcgct ggtgcgcagt gactgatgct gatcggcttg ccatgccact ttcaacacca
     6301 tgttgcgcat gttgtcgatc ttaatcgcca tcagcgccag tttttcctgg atcatctggt
     6361 tatgaccaat gggcttacca aaagcgatac gctggttggc atagcgggcg gcatcttcga
     6421 atgcgcattc ggcaaagccg gtgctgcgtg cggcgttgat caggcgctcc atctcaaagt
     6481 tgtacatcac attgaggaaa cccattcctt cttcgcccac catgtcgctc tcttcaactt
     6541 ccacgttgtc gagatagact tcgcaggtgc tgagcatatg ccagccgatt ttatgcagtg
     6601 gattgatttt aatgccgggt ttactggagt cgacccacca cagagtaaag gcctttttcg
     6661 gatctttcgg ttgcggatcg cgcgccaaca ccagcatata tgggtactct ttcgcaccgg
     6721 taataaaggt tttctgtccg ttgatgtaaa ccttgccgtt tttacgcgtg taagtggtag
     6781 tggcactgtt gttgtctgag cctgcgcctg gctccgtcaa tgccagggca taggcgggat
     6841 cacccgtttc cagggtgctc tctgccgttt tacgtagctg ctctgcagaa ccgaaacggc
     6901 gcatactgtg aatacattga ccgttggtaa tcaaaaatgc cggagcaccg cattttgaca
     6961 cttccatcag cgccagcatt tgggtgacgt aatccgcagg gataccacca aattcttccg
     7021 gcacgccaag catggaaata ccgttatccg ccagtgcccg cataaactca cgcgggtatg
     7081 tcccgttttg atcgcaggtg cggaaatact cttccggaaa gttagtcgta atcagttcgc
     7141 gaatactggc cagcagcagt tcttgttctt cagttaaaga aaaatccatc attatactcc
     7201 tgttaatgag ccgcttcagg caggacaaaa cccatcgccg catcgataaa taatctttcg
     7261 tccatcagtt tgagttctgg cgaaatcact ggggtgaaat ccattttgtc gagaatatct
     7321 ttttgtaaat cgacgccagg agcgatttcg attaaatgca ggccgtcttc ttttaacgtg
     7381 aatacggcgc gctcagtgat ataacgaaca tccagccctc gctcgagagc gatttttccg
     7441 ctgaaagtta tttccggtag ttcccgaata aatttcttca cccgtccttc ctggacgata
     7501 tttaatttgc cgtcggtaat ttctgttttt aaactgcccg cagttaatgt gccgcagaac
     7561 acgattttct tggaagtggc actgatatca ataaatccac cagtgcccat gattttacca
     7621 ttgaatttat gcacgccgac gttaccgtgc tggtcgactt cagcaaaact caaataacaa
     7681 acatccagac caccaccgtg ataaaaatca aactgggacg tcatatccag aatggcgcgg
     7741 gtattcacgt tcgcgccaaa ggcgatcccc tgtgaagtaa taccgccaat cggacctgtt
     7801 tctaccgtca gaataaagtc atcagcacaa ccttcttccc gggcgaccag gccaatgccg
     7861 tcagcaatac cgacgccgac attccccact gcgcctttgc gcatttcgaa taatgcacgc
     7921 cgtgcaacta atttacgttg gtttaggggc aggctaagtt tggtgctgtc atcaagagtg
     7981 aagtcaccag aaataaagcg gttaaccggc gcaccgccat acagttgggt ttgatccgga
     8041 tcgaccacca caatatccac cagataaccc ggaatacgga cagatttagg atgcagcgtg
     8101 gctttcttaa ccattttctg cacctgcatc atcacaatac cgccgttatt gtgcaccgcc
     8161 tgagcaataa ccaatgcgtc gagatacatc acctcatctt caaaagtggc gtagccttcg
     8221 ctgtcgcagg tggtagcgcg aatgaaggcg atatctggcg ctatcgcttt gtaatagaga
     8281 tattctttgt tatcaaactc gaccagttta atcagttctt ctttagtgac ttcattcagt
     8341 ttgccgcctt gctggcgtgg atcgacaaat gtcccgatgc caatatcact aataatacaa
     8401 ggctggtggg ctgcggcggc gcgtaaggtt tgtgtaagta caccttgtgg atagttataa
     8461 gcaataattt tattttgttc tgcgagatca gaaatacgcg gcgattgtcc ccagtgaccg
     8521 cataatgccc atttcaccag accttcttgc gccagcggac taataccacg gtcggcgcga
     8581 tcgccaagcc ctgttggact aataatcgat aaattacgtg gtgtttgagt ctgtttatat
     8641 ttatcagcaa gagcagtaat taacgtggtg gcttccagaa taccgccgcc agcgcctaac
     8701 acacaaagtg ttgcttcgtc gggaatataa ttcaccgctt cctgtgccga caggaccggc
     8761 actcgtccat taatacgagg tggttttaca ggtttcattg ttaattcctg attcaggtgt
     8821 gattttccct acgccagatg gagaattagc gcacagagac tcacgaaata tttttattaa
     8881 atacggttaa aaatactctt tttatttcgg cgggagaggg tattcccgcc gaaatattat
     8941 tgcttatgcc tgatgtaaaa tagttaatac cgtacgcaaa tcatttaccg agatttgccc
     9001 tggcgcagag gcttttttta ccgcaccaaa agttgccgcc gacccaaata cttcaccagc
     9061 cagacgagaa attacgccag tttttgccat cgacatcgtg atgattggac gatcggcata
     9121 ctgttcctgc atctccaggg tcgcggcaag caacgtcagc acatcgctgg tactttgcgg
     9181 catcagcgca atcttaggaa tatcggcgtc gaaggactgc attttgcgca gacgggcaat
     9241 gatttcttcg gcttccggcg ttttatggaa gtcatggttg gacatgacaa ctttcacatc
     9301 atgcgcgtgg gcgtaggcga cggtttcttt aacctgatca tcgccggtaa ataactccag
     9361 atcgatcata tcaaccaggc cactgtcgat ggctgcacga ttgagtgcaa tataagcctc
     9421 ggtggaaatc gcctgctcgc cgccttcttt ggcactgcgg aaggtaaaca gcagcggttt
     9481 ttctggcatg gtttcacgga gaatttttgc tgccgctatg acagactcca cattggagag
     9541 atcggcatag tggtccacac gccattccag aatatcaaag tccgcttcac gataggcgag
     9601 agcttcggat ttcacgctgg cgatatcttt cgccatcagc gagacgatga ttttaggtgc
     9661 gcccgtacca atgacgagat cttttacagt tacggttttc attttttacc ctttctgcac
     9721 gcggtcagcc tgtcaggcac cgaaccccat gacctgttta acatattcca gagggaaatc
     9781 tttgtcagtc cataaagtga actgttcagc cccttgccac aacaacatgc cgtatccatc
     9841 aatcgttttg caacccgctt gttgcgcctg ctgcaataac ttcgtcatat gcgggttata
     9901 cacgcattca gtgaccagaa gtcccggatg taacagactg atatcattaa ccaatgattc
     9961 attctcaagg ggtttcatac ccactttggt gccattggtt aaaatgtcgg cggaagccag
    10021 ggcttcagca aaggcttgct gatcggcgag atcggtgact gtcacgacgc aatcggtgtt
    10081 ttcgttaacc cgctgcgcga aggcgagggc tttatcgaag aactcatccc gacggttaaa
    10141 gagtttaatt tcttttaaac cttcaattgc cccctgcgcg ccaattgccg ttgaggcacc
    10201 tccggcccct aacagcacca tcgttttgcc tttgatatca aaaccgctct ctttaatggc
    10261 gcgaatatgg cccgtgccgt cggtgttata gccacgcaga tagccatcat catttacgat
    10321 ggtgttgatg gcacccacca gtttggctgc tggcgttaat tcatcaacat attcacacgc
    10381 cagttgtttg ttcggcattg atacaccagt tccgcgcatt ttgagggctt ttaatccttc
    10441 aattgctccg ggaaaactat cgttatccac ttcaaaggcc atataggtaa atggcaatcc
    10501 cgctttttct aaggctttat tctgcatttc gggcgataaa ctgtggcgga tgggataggc
    10561 catcaaccca atcaattcgt attttgcggt aacatccata attaactcct ttaacctcta
    10621 tgcttaatcg tgtttaaccg ggtgctaaat ttatgctcgc caaaccgcac atcattttca
    10681 ggaataatga aaacgcggta atagcggata aaaacaataa ttgcggtaat cagggccagc
    10741 agcgcgaaag taaaatcgag aacaatgata tattgcaggc cgatgttcga cagataaccg
    10801 gtaatcagcg gaataacaaa gttagccagt ccacccatca tcatatatat actggtgact
    10861 ttggctttgc ttttggggaa aaactctgac atgaccgaaa cgccgagctg taaaatgccg
    10921 ccagctgctg agaaaccgat aacaaaggct ccggcattac acaccaatgg agaagggtac
    10981 aggtagataa tggcagctgt tattgttgcc agtgcagagt taaatacatt agcccagatg
    11041 ggacggacca tttttttcag tagtgcggca aaaataaaga cacagaccag cgaacccata
    11101 ctgtaataag agatggtttt taatgcctca gcttctgaca taccagcaaa agccatcgca
    11161 tatttgggca tccacaccac aatcacataa aaggtcgaga atgcggctac accgaacagt
    11221 actgatgaaa caccttccag ccagactaac ggtttgctgt tcatttgcgg taattcatta
    11281 gctacgctgg cgtccaccaa ctggctgggg aatttgcttt tcaacagcat cagcgtgatc
    11341 agtacaaaca gaatacccgg aataatcagc ccatagccgt accagatgtt attgagcaac
    11401 atatagctca ccagcattgg gtagaacatt tgcccgaatg acaccatcgc tttaaccagt
    11461 atgaccgccg aaccagaggc tttcggaaag cattccatga gcgcggggta gccacccgta
    11521 tccagcgctg agttagcgat acctacgcac actgccagac agtaggcgag agttaaattc
    11581 gggcaagcag gaataccaaa gaagaatagc agatacatta ttactgccat taatatcacc
    11641 gcccgacgac caaacttatc ggagatcact ccgaagaata aaatactgat caatcgcccc
    11701 aatccgatac cggaaattaa ataggcaatg cccgcgttgt cagtggaaaa cttttctgcc
    11761 aaagatgtca tgttttgggc aagcgtaata acactaatgc cgtgcaggaa atagctgaag
    11821 taaatacaaa gaacagccag gataaatggc gtgctgaaag ccttattttg agacattttc
    11881 acagctcctt gctgaaagat aatttcgtta ccgtacgtat tgttctccga cggtttacat
    11941 aattactata ctgaagatga ttttccctgg gtacatggca taaagtttat gtttccagga
    12001 atattgcatg tcatagtttg tttttgatcg cgattttcct tagttagcgc agaaagagtt
    12061 atttatccgt aagatcttgc ataacctgaa aagaatattg cattacccac ccggagcgat
    12121 attttccttt agcgtgtgat gccgcgtttc tttgcgactg cgtaagccaa taaacagtgc
    12181 cagtataaag ccgatagcag cgatggcggt atcgaaccac ataatatcgg caatacttct
    12241 ttgcgaaaga tgagccgtaa tcaacggaat ggtaaatgtt gcaatgctgc cagcactgta
    12301 atagatcccc gttgcttttc ctttagcgta gggaaaacgt tccgccatta acgtcaggcc
    12361 aatttgtaca acaccgcctg cggaggtaaa accgatgacg aaagcaaata ttatcaccac
    12421 ataaaatgtg ggatgcaggc agacggtgag cagggcgata aatgagataa aggtgtacag
    12481 catcagtaat gttgttgggc gaacggtatt acgaataagt ggagcagtaa taaatacaca
    12541 cagcagcgaa cccacggtgt agatactgag taatttgatc gacatggtgt atgacatgcc
    12601 agcgacaaat tgtccgtatt gtgccagcca ctggctaacc agataaaacg ttgccattga
    12661 gatatagcca tatagggaat aactggctaa atcgataatt gaacagcgat gttccgtaga
    12721 gctggtggtt ttctttatga caggtaagtg acgacccgga tgaggtggga acgtacaacg
    12781 gtataaaaac agagcgttaa taaacataat gcctgcggca atcataaagg accaaccgaa
    12841 ccacagttca gcccacacta acaggctgat aattagcggt aataaaaact gtccgctgga
    12901 aacaaatgct ttaattaaaa tattggctgt acccggtgag cgtggaaaag cttccatcaa
    12961 actgggatag gtgcctgcat cgagaaaact gtttgccatt cccgccagaa agccaaaaac
    13021 ataagcgata atgatgttat tggtgtgcag gatgccaaaa aagaaggcca tatagcagca
    13081 catcccgagc atgataaaag ggcggcgacc aaagcgatcg gataataatc ctgcaaaaag
    13141 caagacactt aatcgaccaa tgcccagtga tgagataact atcgagacgc ccgcggcatt
    13201 agtttgccaa agcgtctcca gcgaggccat attcaggctc atcaaaatga cgcctatacc
    13261 atgcaccagg taattaaaat acaacccaag tgcggtaggg taatagggat ttttcatagg
    13321 ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat ccgttcatta
    13381 ttgttgtaac aacgacaatt cctttctgtg gagaaattaa caaagccgct ccagatcgtg
    13441 tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat tgccatcagg
    13501 gtagacttgt tgaaacgcgg ttaagtcggg gaaaaaacgc atggtgttgt tgccgatgcg
    13561 gttatttatt ttctctatga tggcatgaag atgttttttt cgttgctgac gcatagccaa
    13621 cagttgctcg actacacaat caggaatggc gcatttgcca ttttcccact gacgccagct
    13681 ttcactgtta ccggtctggg caatccaggt cgcgcattca tcaatagtca tggcaaaaat
    13741 atggcgaagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg aaataaacaa
    13801 taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca gtatcttgtg
    13861 caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt aaaaaaaagc
    13921 ccatcgtggg agatgggcaa agactacaca cagcaattcg ttgtttcact caggggattt
    13981 ccatgcttat aaatcaatat gttgatttat aaccgtgagc taatagtagg catacggact
    14041 ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt gcgacaggta
    14101 ctaccagctg aatagcggta agcaggaaaa tatggggaag agcaagcaaa tatcaattag
    14161 caggggttaa caatcaggcg gcagagccgc ctgatgagaa attacttatt cgatttctcg
    14221 atttcgccaa gttcttcgag aatttcttcc ggttggtccg tcggcggtgg gacttcttct
    14281 acccacgcgg caaaaagacg ccaggaaacg gctaacagaa ccggaccaat aaacagaccg
    14341 atcatcccga aagcaatcaa accaccaata acgccagaga ggatcaggat cagcggtaaa
    14401 tcggcaccca tgcgaattaa cattgggcgg atgacgttat cgagcgtgcc aaccacaccg
    14461 ctccaaacta acaataccgt tccccaggtg gtatcgccag tccagtagag ccagataatc
    14521 gccggaatca gtaccggcaa cggtccaagc tggacaaggc aggagaggat cattaacacc
    14581 gttagcaaag ttgcataagg tacgccggat acggcgaggc cgataccgcc aagcactgcc
    14641 tgtactaacg ccgtcaccac cacacccagc gccaccgcgc ggatagcctg tgccgccagc
    14701 agcacggcgg catctccgcg aacgcctgcc agacgggttg caaaatggcg aatgccttgt
    14761 gccacctgtt caccgcgcca gtacagcagg gcgctgaaga gaagcatcag cgcacaatgc
    14821 accataaagc gcccgatatg cgccgcctgc ccaacgaacc aggtggtggt ggtgccaata
    14881 taaggacgga ctttcgccat gatcgccgtc ccccccatat ccagcaaatt gtgccagcct
    14941 gcatatagct tcgcgccaat caccggaatg gtattaagcc acgccagatc gggtaacgtc
    15001 atgtcaccac tggaaatggc tttaattagc gggccgctgc cgtcgacgat actgttaacc
    15061 agcaaagcaa tagggatgat aaacaccatc actaataaca gcgtcattac cagaacggcg
    15121 agagagcggc gaccaaacat gatcttttgc aaacgtaaca ataccggcca ggtggcgata
    15181 accactgtac cggcccatgc aaagccgaga ataaagggtt gtacaatcca cagacatgcc
    15241 acaatcatga tggctaaaaa cagaaccgaa agcagaattt gtgcgacatc cctgggctga
    15301 cgaacattta ccataactac ttttcacctt ttttgtgcgt caaatcgtcg acgcgacgtg
    15361 aacacgcgaa actcacctgc ataatgatga gcaatttcag cggtttttaa caggtcgatt
    15421 ctgcccttaa ttctgatggg cgtgttagaa aaaaatgtgg tacaacaact caggcaacac
    15481 gcaaacgatt aatctctcat cacagaccgc aggaaagggt caatataatg attccacaga
    15541 tttcccaggc acccggcgtc gttcaactgg tgcttaattt tttgcaagag ctggagcaac
    15601 agggttttac cggcgatacg gcgacaagtt atgccgatcg tctgacaatg tcgactgaca
    15661 acagtattta ccaacttctc cccgatgcgg tggtatttcc gcgttcaacc gcagatgtag
    15721 cgctgatcgc ccgtcttgcc gcgcaggaac gctattcatc gttgatcttt accccccgcg
    15781 gcggcggcac cggcactaac ggtcaggcgc tcaaccaggg gattattgtt gatatgtccc
    15841 gccatatgaa ccgcatcatc gaaattaacc ctgaagaggg ctgggtgcgc gttgaggccg
    15901 gggtgattaa agaccaactc aatcagtacc tgaaaccctt tggctacttt tttgcgccag
    15961 aactttcgac cagtaaccgg gcaacgctcg gcgggatgat caataccgat gcatccggtc
    16021 agggatcgct ggtctatggc aaaacgtcag atcacgtact tggcgtacgc gcggtgttgt
    16081 tggggggcga tattctcgat acgcaacctt tacccgtcga actggcggaa acgctgggta
    16141 aatccaatac cacaatcggg cgaatttata acacggttta tcaacgttgc cgtcagcaac
    16201 gccagttaat catcgacaac ttccccaagc ttaaccgctt tcttaccggt tacgatctgc
    16261 gtcatgtctt taacgatgag atgaccgagt tcgacctgac gcgcattctg acgggttcag
    16321 aagggacgct ggcctttatt accgaagcgc gactggatat tacgcgcttg cctaaagtac
    16381 gccgtctggt gaacgtcaaa tatgactctt ttgactccgc gctgcgtaac gcgccgttta
    16441 tggttgaggc gcgggcgctt tcggtagaga cggtggactc aaaagtgctg aatctggcgc
    16501 gggaagatat tgtctggcat tccgtcagcg agttgattac cgatgtgcct gacaaagaaa
    16561 tgctcgggct gaacattgtg gaatttgctg gtgatgatga gacgctgatt gatgagcgag
    16621 taaatgcact ctgtgcgcgg ctggatgaac ttatcgccag tcagcaagca ggtgtgatcg
    16681 gctggcaggt atgcagtgag ctggcaggtg ttgagcgtat ttatgcgatg cgcaaaaaag
    16741 ctgtcggcct gctcggtaat gccaaaggag cagccaagcc aattccgttt gctgaggata
    16801 cctgcgtacc gccggaacat ctggccgatt atattgctga atttcgcgcg ctgctcgaca
    16861 gccacggctt aagctacggt atgttcggtc acgtcgacgc aggtgtcttg cacgtccgtc
    16921 ctgcgctgga tatgtgcgat ccgcaacaag agattttgat gaagcaaatc tctgatgacg
    16981 tggtggcgct gactgcgaaa tacggtggtt tgttgtgggg cgagcacggc aaaggttttc
    17041 gcgctgaata cagcccggcg tttttcggtg aggaactttt tgcagaactg cgcaaagtga
    17101 aagcggcatt tgacccgcat aaccgactca acccagggaa gatttgcccg ccagaaggtc
    17161 tcgatgcgcc gatgatgaaa gtggacgcgg tgaagcgcgg tacattcgat cggcagatcc
    17221 ccattgcggt acgccagcag tggcgcggtg caatggagtg taacggcaac ggtttatgct
    17281 tcaactttga tgctcgtagt ccgatgtgtc cgtcgatgaa gatcacccag aaccggattc
    17341 attcaccgaa agggcgcgca acgctggtgc gtgaatggct gcgtttgttg gcggatcgcg
    17401 gcgttgaccc actcaaactg gaacaagaac tgcctgaatc cggcgtcagt ttgcggacgt
    17461 taattgcccg cacgcgcaat agctggcatg cgaataaagg cgaatatgac ttctcacacg
    17521 aagtcaaaga ggcgatgtcg ggctgtctgg cctgtaaagc ttgttcgacc cagtgcccca
    17581 tcaaaattga tgtgccggag tttcgctctc gttttctgca gctctatcac acccgttatt
    17641 tacgccctct gcgcgaccac cttgtcgcta cggtcgagag ctacgcaccg ctgatggcac
    17701 gcgcgccgaa aacctttaac ttcttcatta accagccgct ggtgcgcaaa ctctcggaaa
    17761 aacatattgg catggttgat ttgccgctgc tgtcggtccc ctcgctgcaa caacaaatgg
    17821 tggggcatcg ctcggcgaat atgacgctgg aacagcttga agctctcaat gcagagcaga
    17881 aagcgcgcac ggtgttggtg gtgcaggacc cctttaccag ctattacgat gcgcaagtgg
    17941 tggcggattt tgtccgtctg gtcgaaaaat taggtttcca gcctgtgtta ctgccatttt
    18001 cgccaaatgg caaagcccag catatcaaag gctttcttaa tcgttttgcg aagacggcga
    18061 aaaagacggc ggatttcctc aaccgtatgg cgaagctggg gatgccaatg gtgggcgtcg
    18121 atccggcgct ggtactttgt tatcgcgatg aatataaact ggcactgggc gaagaacgtg
    18181 gcgagtttaa cgtcttactg gcgaatgaat ggctggcaag cgcacttgag tcacagccag
    18241 tggctacagt cagcggtgaa tcatggtatt tctttggtca ctgtaccgaa gttaccgcct
    18301 tgccgggcgc gccagcacaa tgggccgcga tatttgcccg ttttggcgcg aaactggaaa
    18361 atgtcagcgt gggttgctgc ggcatggcag ggacttacgg acatgaagcg aaaaaccata
    18421 aaaattcgct cgggatctat gagttatcct ggcatcaggc gatgcagcga ctaccgcgta
    18481 accgctgtct ggcgaccgga tattcctgcc gtagtcaggt aaaacgggtt gaaggcacag
    18541 gggtacgcca tcctgtgcag gctttactgg agattattaa atgatatgga aacgaaaaat
    18601 caccctggaa gcactgaatg ctatggggga aggaaacatg gtgggattgc tggatattcg
    18661 ctttgaacat attggtgatg acacccttga agcgacaatg ccagtagact cgcggacaaa
    18721 gcagcctttc gggttgctgc atggaggggc atccgtggta ctggccgaaa gtatcggttc
    18781 cgttgccggt tatttatgta ccgaaggtga gcaaaaggtg gttggtctgg aaatcaatgc
    18841 taaccacgtc cactcggcac gagaagggcg ggtgcgcggc gtatgcaaac cgttgcatct
    18901 cggttcgcgt caccaggtct ggcagattga aatcttcgat gagaaagggc gtttgtgctg
    18961 ttcgtcacga ttgacgaccg ctattttgtg acgttgtcac cagaaaagtg tgacgcatat
    19021 tgcttttggt tattatctgc acagtaaacg gtggtatact gtgcaggtct tgtctaaaag
    19081 cgaggatgct atgtctactc aacttgatcc cacccaactg gccattgaat ttttacgccg
    19141 tgaccaaagc aatctttcac ctgcccaata tctcaagcgt ctgaaacaat tagaacttga
    19201 atttgctgac ctgcttactc tctcttctgc tgaattaaaa gaagaaatct acttcgcctg
    19261 gcgtctgggc gtgcattgat cctgatttaa tgtcgcgagt tcgccaaatc ggcctggatt
    19321 aatcttcgtc caggttcgag ggccttttta atacagccta tctacccgcc tgataacgtt
    19381 caaaaataat acaaatcgca gtttgtgtta aaacagtggg ttagctttat gagattatcc
    19441 ttatcgcccc ttcaagagct aagccactga gagtgccgga gataagcgcc ggatggggta
    19501 gaaaccctta agcctgtgtc gcacagactt aagggttttc ttatttctga acgctgcact
    19561 tttttaccta tctcttaaat agtcattttc aatacaactt tttctttttc atcacagcct
    19621 taatgctcct tagataataa tcattatcta acaatgagat acctaattct tagcgtgcct
    19681 gttaacccac acatcagggt ctatgcttat taaaagcagg taaaaagcat gttttacgct
    19741 aaagcccctg cgtttgctgg gttgaactga taatcattat cactaacatg ctgttatacg
    19801 ctgaaagcga tgaagtgagg taaatcgatg gacatgcctt caggaacctt taatccacag
    19861 gatttcgcct ggcaaggctt aacgctgaca cccgcagcgg cgatacacat ccgcgaactg
    19921 gtggcaaagc agcctggtat gatcggcgtg cgcttaggcg ttaagcaaac gggctgcgcg
    19981 ggctttggct atgtgctcga cagtgttagc gagccggaca aagacgatct gctgtttgaa
    20041 cacgacggcg cgaagctgtt tgtcccgctg caagcgatgc cttttattga tggcacggaa
    20101 gtcgatttcg ttcgtgaagg acttaatcag atattcaaat ttcacaaccc taaagcccag
    20161 aatgaatgtg gctgtggcga aagctttggg gtataggcgg tactatgtct cgtaatactg
    20221 aagcaactga cgatgtcaaa acctggaccg gcggcccgct gaattataaa gaaggattct
    20281 tcacccagtt agccaccgat gagctggcaa aggggataaa cgaagaggtg gtgcgcgcaa
    20341 tttcggcgaa gcgtaatgag ccggagtgga tgctggagtt tcgtctcaac gcctatcacg
    20401 catggctgga gatggaagaa ccgcactggt tgaaagcgca ctacgacaag ctgaattatc
    20461 aggattacag ctactactcg gcaccatcgt gcggtaattg tgacgacaac tgcgcgtctg
    20521 aacccggcgc ggtgcagcaa actggcgcga acgccttttt aagtaaagag gtggaggcgg
    20581 cgtttgagca gttgggcgtt cccgtgcggg aaggcaaaga ggtggcagtg gatgccattt
    20641 tcgactctgt ttcggttgcc actacttatc gcaaaaaact ggcagagcag ggaattattt
    20701 tttgttcctt tggcgaggcg atccacgatc acccggaact ggtgcgtaaa tatctcggca
    20761 ccgtggtgcc ggggaatgac aacttctttg ccgcacttaa tgcggcggta gcctctgacg
    20821 gtacgtttat ttatgtgcct aaaggcgtgc gttgcccgat ggaactttcc acctattttc
    20881 gcattaacgc ggaaaaaacc gggcagtttg agcgcaccat tctggtggcc gacgaagaca
    20941 gctacgttag ctacattgaa ggctgttccg ctccggtgcg tgacagctat cagttacacg
    21001 cggcggtggt ggaagtcatc atccataaaa acgccgaggt gaaatattcc acggtacaaa
    21061 actggttccc tggcgataac aacaccggcg gtattctcaa cttcgtcacc aagcgtgctt
    21121 tgtgcgaagg cgaaaacagc aaaatgtcat ggacgcaatc agaaaccggc tcagcgatta
    21181 cgtggaaata tcccagctgc attttgcgcg gcgataactc cattggtgag ttttactcag
    21241 tggcgctgac cagcggtcat cagcaagcgg ataccggcac caagatgatc cacatcggta
    21301 aaaacaccaa atcgaccatt atctcgaaag ggatctctgc cggacatagt cagaacagtt
    21361 atcgcggctt agtgaaaatc atgccgacgg caaccaatgc gcgcaatttc actcagtgcg
    21421 actcaatgct gattggcgct aattgtgggg cgcatacctt cccgtatgtc gaatgtcgca
    21481 ataacagcgc tcagctggag cacgaggcaa cgacatcacg tattggtgaa gatcaactgt
    21541 tttactgcct gcaacgcggg atcagcgaag aagatgccat ctcgatgatt gttaacggtt
    21601 tttgcaaaga cgtgttctcg gaactgccgc tggaatttgc cgttgaagca caaaaactcc
    21661 tcgccatcag tcttgaacac agcgtcggat aaggaataaa catgttaagt attaaagatt
    21721 tacacgtcag cgtggaagat aaagctatcc tgcgtggatt aagcctcgac gttcgtcccg
    21781 gcgaagttca cgccattatg gggcccaatg gttcgggcaa aagtacctta tcggcaacgc
    21841 ttgccgggcg agaagattat gaagtgacga gcggcacggt tgagtttaaa ggcaaagatt
    21901 tgcttgcgct gtcgccggaa gatcgtgcag gtgaaggcat ctttatggcc ttccagtatc
    21961 cggtggaaat tcccggcgtc agtaaccagt ttttcctgca aacggcactt aatgcggtgc
    22021 gcagctatcg cggccaggaa acgctcgacc gctttgattt tcaggatttg atggaagaga
    22081 aaatcgctct cctgaagatg ccggaagatt tattaacccg ttcggtaaac gttggttttt
    22141 ccggcggcga gaaaaagcgc aacgatattt tgcaaatggc ggtgctggaa ccggagttat
    22201 gcattcttga tgagtcagac tccgggctgg atattgacgc attaaaagtg gttgccgatg
    22261 gcgtgaactc gctgcgtgac ggcaagcgtt cattcatcat tgttacgcac taccaacgca
    22321 ttctcgacta catcaagcct gattacgtcc atgtgctgta tcagggacga attgtgaaat
    22381 ccggcgattt cacgttggtc aaacaactgg aggagcaggg ttatggctgg cttaccgaac
    22441 agcagtaacg cactgcaaca gtggaatcat ttgtttgaag ctgaaggggc gaaacgctcc
    22501 ccgcaagcac agcagcattt acaacaattg ctgcgtaccg gactgccgac acgtaaacat
    22561 gaaaactgga aatatacgcc gctggacggg ctgaccaata gccagtttgt cagcattgcg
    22621 ggggagatag ccccacagca gcgtgatgcc ttatcgttaa cgttagacgc cgtgcggctg
    22681 gtgtttgtcg atggacgtta cgtgcccgca ctgagcgatg caactgaagg cagcggatat
    22741 gaagtgagca ttaacgacga ccgtcagggg ttacccgacg ctattcaggc ggaagtgttt
    22801 ctgcatttga cggaaagcct ggcacaaagc gtgacgcata tcgccgtgaa gcgcggtcaa
    22861 cggccggcaa agccattgct gttaatgcat atcacccagg gcgtggcagg tgaagaggtg
    22921 aatactgccc attaccggca tcatctggat ctggcggaag gtgcagaagc aacggtgatc
    22981 gaacattttg tcagccttaa tgatgctcgt cacttcaccg gcgcacggtt cactatcaac
    23041 gtcgcagcga acgcccattt gcagcatatc aagctggcgt ttgaaaaccc gctcagtcac
    23101 cactttgctc ataacgattt gttgctggct gatgatgcca ccgcatttag ccacagtttc
    23161 ctgctgggtg gcgcagtgtt acgacacaac accagtacgc aactcaatgg cgaaaacagc
    23221 acggtgcgga tcaatagcct ggcgatgccg gtgaaaaacg aggtgtgtga tactcgtacc
    23281 tggctggaac acaataaagg tttttgtaac agccgacagt tgcacaaaac tatcgtcagc
    23341 gacaaaggcc gcgcggtatt taacggtttg atcaacgtcg cgcagcacgc catcaaaacg
    23401 gatggtcaga tgaccaacaa taatctgctg atgggcaaac tggcggaagt ggatactaaa
    23461 ccgcagctgg aaatctatgc agatgatgtg aaatgcagcc acggcgcgac ggtggggcgt
    23521 attgatgacg aacagatgtt ttatctgcgc tcgcgcggga tcaatcagca agatgcccag
    23581 cagatgatca tctacgcctt tgctgccgaa ctgacggaag cactgtgtga tgaggggctg
    23641 aaacagcagg tgctggcccg aatcggtcaa cgactgccag gaggtgcaag atgacttttt
    23701 ccgtcgacaa agtgcgggcc gactttccgg tgctttctcg tgaggtaaac ggtttgccgc
    23761 tggcttatct cgacagcgcc gccagtgcgc agaaaccgag ccaggtgatt gatgccgagg
    23821 ctgagtttta tcgtcatggc tacgcggcgg tgcatcgcgg tattcatacc ttaagcgccc
    23881 aggcgaccga gaaaatggag aacgtgcgca agcgtgcttc gctgtttatt aatgcccgtt
    23941 cggcggaaga gctggtgttc gtccgcggca cgacggaagg gatcaatctg gtcgccaata
    24001 gctggggcaa cagcaatgtg cgagcgggcg ataacatcat catcagtcag atggagcacc
    24061 acgctaacat tgtcccctgg cagatgcttt gtgcacgcgt tggcgcagag ctgcgtgtga
    24121 tcccgctcaa ccccgacggt actctgcaac tggagacgct gcctacgctg tttgatgaga
    24181 aaactcgtct gctggcaatt actcatgtct ccaacgtgct tgggacagaa aatccgctgg
    24241 cggagatgat cacacttgcg catcaacatg gcgcaaaagt gctggtggat ggcgctcagg
    24301 cggtgatgca tcatccggtg gatgttcagg cgctggattg cgatttttac gtgttctctg
    24361 ggcataaact gtatggccct accggaattg gcattcttta tgtcaaagaa gccttgttgc
    24421 aggagatgcc gccgtgggaa gggggcggtt ctatgatcgc caccgtcagc ctgagtgaag
    24481 gcactacctg gaccaaagca ccatggcggt ttgaagccgg tacacccaat accggaggca
    24541 tcattggtct tggcgcggcg ctggagtatg tttcggcgct ggggcttaat aacatagccg
    24601 agtatgaaca gaatctgatg cattacgcgt tatcacagtt ggaatctgta ccggatctca
    24661 ctctctatgg cccacaaaac aggcttggcg ttattgcttt taatctcggt aaacaccacg
    24721 cctatgatgt tggcagtttt ctcgataatt acggcattgc tgtgcgtacc ggacatcact
    24781 gcgcaatgcc attaatggcc tattacaacg tccctgcgat gtgtcgggca tcgctggcca
    24841 tgtataacac ccatgaagaa gtggatcgtc tggtgaccgg cctgcaacgt attcaccgtc
    24901 tgctgggata acagggaggc actatggctt tattgccgga taaagaaaaa ttgctgcgta
    24961 attttttacg ctgcgccaac tgggaagaga aatatctcta cattattgag ttgggtcagc
    25021 gtctgccaga attacgtgac gaagacaaaa gcccgcaaaa tagcatccag ggctgccaga
    25081 gtcaggtgtg gattgtgatg cgtcaaaatg cgcagggaat tattgaatta cagggcgaca
    25141 gcgatgcggc cattgtaaaa gggcttattg cggtcgtctt tattctttac gatcaaatga
    25201 cgccgcagga tattgtcaat ttcgatgtgc gcccgtggtt tgaaaaaatg gcgctcaccc
    25261 aacatctcac cccatcacgc tcacaaggtc tggaagcgat gattcgcgca attcgcgcca
    25321 aagccgctgc acttagctaa actaaaggga cagctttcat ccggttggct cacattgccg
    25381 ggtggtcaat gtttacactg gcctgcacag gagtgttttg gtttcaggtg aacataagcg
    25441 ccatccgacg tttcagcgtg agtctccggc aaatacagga ggtttacaat gaaacgcgcg
    25501 tctttgctta cactcacgct tatcggcgct tttagcgcca tccaggctgc ctgggcggtt
    25561 gattatccgc taccaccaac cggcagtcga ctggttgggc aaaatcaaac gtatacggtg
    25621 caagaagggg ataaaaacct tcaggccatc gcccgacgtt ttgatactgc ggcaatgttg
    25681 atccttgaag ccaataacac tatcgccccg gtgccaaaac ccggtacgac gataactatt
    25741 ccttcacaac tattattacc tgatgcgccg cgtcagggga ttattgtaaa ccttgcagag
    25801 ctgcgccttt ataattatcc gccgggagaa aatatcgtgc aggtttatcc gataggtatt
    25861 ggtttgcagg ggctggaaac gccggtgatg gaaacgcgtg tggggcagaa aatccctaac
    25921 ccaacctgga cgcctacggc gggcattcgt caacgttcgc tggaacgtgg cattaaatta
    25981 ccaccagtcg ttcctgccgg gcccaataac ccactaggac gttacgcact gcgcctcgcg
    26041 catggtaatg gcgaatacct cattcatggc accagtgcgc cggacagcgt cggtttgcgc
    26101 gtcagttcgg gctgtattcg catgaatgca ccggatatta aagctttatt ctccagcgtg
    26161 cggacgggaa cgccggtgaa agtgatcaac gagccggtga aatattccgt ggaacctaac
    26221 gggatgcgtt atgttgaagt acatcgacca ctatcggcag aagaacagca gaacgttcag
    26281 acaatgccgt acacgctgcc agcaggcttt acgcaattta aagacaataa ggctgtagat
    26341 cagaagttag tcgataaagc gttgtatcgt cgggcagggt atccggttgc ggtgagcagt
    26401 ggagcaactc ccgcagccag caatgcgcct tcagtagagt cagcgcagaa tggggaacca
    26461 gagcaaggga atatgttacg cgcgacgcag tagcggtaaa tagcaggcaa aaaaatggcg
    26521 cacaatgtgc gccatttttc acttcacagg tactattact tgcggtattt agtagccatg
    26581 ttgtccagac gctggttagc acgagctgcg tcatctttag cagcctgaac gtcggaacgc
    26641 attgcgttca cgtcgttgct cagctggtca actttagcgt tcagagtctg aacgtcagaa
    26701 gacagctgat cgattttagc gttgctggag caacctgcca gcagagtaga acccaggatt
    26761 accgcgccca gtaccagttt agtagctttc attattaata ccctctagat tgagttaatc
    26821 tccatgtagc gttacaagta ttacacaaag ttttttatgt tgagaatatt tttttgatgg
    26881 gaaggcactt atttttgatc gttcgctcaa agaagcatcg aacgctgggt tttagataaa
    26941 aaaattgaga gaaaacagga tgcttccatc ggattcatct tagataaagt gagattatat
    27001 agtgaaatcc gatttgattt tttattgctt ctggttatcg attaaataaa aaaagcgccc
    27061 atcagggcgc ttcgatatac aaattaattc acaaaagcaa tattacagga cgtgaacaga
    27121 tgcagtgtta gtagtgccgc tcggtaccag tgcaccagaa accataacta cgacatcacc
    27181 tttgtgtgcc agaccgctct gcagagccag ttctttaccc agacggtaga agtcatcagt
    27241 agaagtgatc tctttaacca gctgcggcac aacgcctttg ctcagtacta actggtgagc
    27301 tgttttttcg ttggtggtca gtgccaggat ggtggcatcc gggaagtatt tacgtactgc
    27361 gcgagcagat ttaccgccct gagtagcaac cacgatcagt ggagcatcca gtttttcagc
    27421 agtttcaacg gcaccacggc ataccgcttc ggtaatacgc agtttacggt tgtcattgtt
    27481 gaactcgaga cggctgttca tcacgcggtc ggtacgttcg cagatggtcg ccatgataga
    27541 aaccgcttcc agcgggtatt taccttttgc ggattcacca gacagcatca ctgcgtcagt
    27601 accgtcgagg atggcgtttg caacgtcacc ggcttctgcg cgagtcgggc gtggattttt
    27661 gatcatggaa tccagcatct gggtcgcagt gataacaact ttacgtgcac ggatacattt
    27721 ttcgatcatc atcttctggg cgaagataac ttcttctacc gggatttcta cacccaggtc
    27781 gccacgcgca accatgatgc cgtcagaggc ttcgaggatt tcgtcgaagt tgttgaggcc
    27841 ttcctggttt tcgattttgg agatgatgtg gatgttttcg ccgccgtgcg ctttcaggtg
    27901 ctcacggatt tcgataacgt cagaacgctt acgaataaag gaagcagcaa caaagtctac
    27961 gccttgttcg caaccaaaga tcaggtcctg tttgtctttt tcagccagtg ctggcagggc
    28021 aatggaaacg ccaggcaggt tcacaccttt gttttcgccc aggtcgccgt tgttcagcac
    28081 tttacagata actttgttac cttcaatggc ggtaacttcc ataccgatca gaccgtcgtc
    28141 aaccagtacg gtgttgccaa cagacaggtc agtagtgaaa ccttcatacg ttaccgcaac
    28201 catttcgctg ttgccgataa cagatttatc agtagtgaaa gtaaaggact gacccgcttt
    28261 cagagaaacg tcgttaccgc cttccagttt catggtgcgg atttctggac ctttggtatc
    28321 aagcaggatg gcggcggttt taccagtttt gctcatcacg ttgcgcagat tctgaatgcg
    28381 ctgaccgtgt tctgcatagt caccatgaga gaagttcaga cgcataacgt tcatgccagc
    28441 gtccagcatt ttagctaaca tctcttcaga ttcggttttc ggtccgatgg tgcaaacaat
    28501 tttggtcttt ttcatgacag tcttagtctt taagttgaga aggatgggag aaacttgctt
    28561 tctgggcgca ctgccggaaa gcgaatccgg tattacgaaa gtggttgtgc ctcgctctaa
    28621 ggataggtga catcgaaaaa gcgtgcagag gaatgtgtgc ttgtgtctca gcccaacgtg
    28681 agtgcctgtt tttgagtcgt tgagttctac agtccaaagt gctgaaacca ttcaagagtc
    28741 aattggcgcg cattatacgc tgaaattaat ccaaaaggaa aacaaaaaca gcgtttcaaa
    28801 aaatatacaa cttgtaagac aataaattgt tatcaaagat tcagcgttcc agggaaaagg
    28861 ttacgcaaaa acttcgttgt ttgcgcaacg tctggtgccg gggttgtcag tgtgaggata
    28921 agtgaaaagt tcatccggtg tataaataca tcatcgctca attgctacag ttaaaataat
    28981 ttatcacgtt atttgtctga ttttgtttga tttttgttta attggttaga ttgatatatg
    29041 ctgatagttg gcatgataaa taggtataaa gaatgggaaa ccggacaaaa gaggatgaac
    29101 tgtaccgaga aatgtgcaga gttgtcggta aagttgtgct ggaaatgcgc gatctggggc
    29161 aggaaccaaa gcatattgtt atcgcaggcg tattacgtac agcattagcc aataaacgca
    29221 ttcagcgcag tgagctggaa aagcaggcga tggaaacagt cattaacgca ctggtaaaat
    29281 gaccattcag gatgaggcgg taagcttcat cctgatattg ttagttcagt tgcggtccag
    29341 ttaatcattc taaataacaa ctgctgaaat tatatgaatt tcggtgtgta ttttaattta
    29401 tagcgataaa gttattttta ttttctgcga tgaacttcac aaataattgt cggttaagtt
    29461 aaatttattt caaatgataa taaatatcag ttcacttata attccttgct ttgtgatttt
    29521 aattgataac gatcaatgtt aagagcccag aataatcaca ttattttttc tcctaaccat
    29581 aaaggattag tttgtcagcg ttaaatcttt tctcatacac tatcgggttt gggaaaatat
    29641 ctggtgttga ggacagagtc agcagcgtat tagttgagtg cctaatatca ggctgctgaa
    29701 ttgtcagcac tgtgtttgcg aagaggatgc gaaataaatg aacccggttg atcgtccact
    29761 attaaatatt ggcttaacgc gattagaatt cttgcgtata tcaggaaaag gccttgctgg
    29821 tttaacaatt gcccctgctt tactgtcact tttgggttgc aaacaggaag atattgatag
    29881 cggcacagta gggttgataa atacacccaa aggggtgctg gtgacccaac gtgcgcgatg
    29941 cacgggttgc caccgctgtg aaatctcttg taccaacttc aacgatggct cagtagggac
    30001 attcttctcc cgtatcaaaa tccatcgcaa ttatttcttt ggcgacaacg gggttggctc
    30061 tggcggtggc ctgtatggcg atctcaacta taccgcggat acctgccgtc aatgcaaaga
    30121 gccgcaatgc atgaacgtct gcccgattgg tgcgattacc tggcagcaga aagaaggctg
    30181 tattaccgtc gatcataaac gttgtattgg ctgtagcgcc tgtacgacgg catgcccgtg
    30241 gatgatggcc accgtaaata ccgaaagtaa aaaatcctcg aaatgtgtgt tatgcggtga
    30301 atgcgcaaac gcctgcccaa caggggcgtt aaaaattatc gagtggaaag atattactgt
    30361 gtgaatcttg caaaggaaaa aatcatggct aacggttgga caggtaatat attaagagtc
    30421 aatctcacga caggaaatat taccctcgaa gattccagta agtttaaaag ttttgtcggt
    30481 ggcatgggct tcggctacaa aattatgtat gacgaagtac cgccaggcac gaaacctttc
    30541 gatgaagcga ataaattagt ttttgctacc ggcccattaa ctggatctgg tgccccctgt
    30601 agttctcgcg taaatatcac ttcactttct acttttacca aaggaaattt agtcgtcgat
    30661 gcccatatgg ggggcttttt tgcagcgcaa atgaaattcg ctggatacga cgtcattatt
    30721 atcgaaggga aagcgaaatc accggtatgg ctgaagatta aagatgacaa agttagcctt
    30781 gaaaaagccg atttcttatg gggaaaaggg acgcgcgcaa cgacggaaga aatttgtcgc
    30841 ttgaccagtc cggaaacctg tgtggcggct attggtcagg ctggggaaaa ccttgttcct
    30901 ctctctggca tgttgaatag ccgtaaccac agcggcggtg cgggaactgg cgcaataatg
    30961 ggttcgaaaa acctgaaagc gattgcggtt gaagggacga aaggggtcaa cattgccgat
    31021 cgtcaggaga tgaagcgtct caacgattac atgatgactg aacttatcgg tgcgaataac
    31081 aaccatgtcg tgccaagtac gccgcaatcg tgggcagagt attcagatcc caagtcacgt
    31141 tggacagcac gtaaagggct gttttggggc gcggctgaag gtggtccgat tgaaacgggg
    31201 gaaattcctc caggcaatca gaacacggtc ggctttcgta cctataaatc cgtttttgac
    31261 ttaggtccgg cggcagagaa atacacagta aaaatgagtg gctgccactc ttgcccgatc
    31321 cgttgtatga cccaaatgaa tattcctcgg gtgaaagagt ttggcgtgcc cagcacaggt
    31381 ggtaacactt gtgtagcaaa ctttgtccat accaccatct tcccgaacgg gccgaaagat
    31441 tttgaagata aagatgatgg tcgtgtgatt ggtaacctgg ttggtctgaa tctgttcgat
    31501 gactacggcc tatggtgtaa ctatggtcag cttcatcgcg actttaccta ttgttacagc
    31561 aaaggggtgt tcaagcgtgt tctgcctgct gaagagtatg cggaaattcg ctgggatcaa
    31621 ctggaagcgg gtgacgttaa cttcattaaa gatttttact accgtctggc gcatcgcgtg
    31681 ggtgagctga gtcacctggc tgatggttca tatgccatcg cagaacgctg gaatttgggt
    31741 gaagagtact ggggctacgc gaaaaataaa ctctggtcgc cgtttggcta tccagttcac
    31801 cacgccaatg aagcgtcagc gcaggtcggc tccattgtta actgtatgtt caaccgtgac
    31861 tgcatgacgc atacccatat caattttatt ggttccggct tgccattgaa attgcagcgt
    31921 gaagtggcga aagagctttt tggttctgaa gatgcttacg atgaaaccaa aaactacacg
    31981 ccaatcaatg acgcaaaaat taaatatgct aagtggtcgc tgttgcgggt ctgtttgcat
    32041 aacgccgtca ccctgtgcaa ctgggtctgg ccaatgaccg tttcgccgct gaaaagccgt
    32101 aattatcggg gcgatctggc gcttgaagcc aaattcttca aagcgatcac tggcgaagat
    32161 atgactcagg aaaaattaga tttagctgca gagcgtatct ttacgttgca tcgcgcctac
    32221 acggtaaaac tgatgcaaac caaagatatg cgtaatgaac acgatcttat ctgttcctgg
    32281 gtattcgaca aggatccgca gattccggtc tttactgaag gtacagacaa aatggatcgt
    32341 gacgatatgc atgcttcgct cacgatgttc tacaaagaaa tgggctggga cccacagctt
    32401 ggttgtccga cccgcgaaac attgcagcgt ctggggctgg aagatattgc cgccgatctg
    32461 gcagcacaca atctactgcc tgcgtaagga atgggagaga tgaaccatca ggatgaattg
    32521 ccgttagcgg aagtcagcga aattgatgag gcgaagcggc aatggttgca ggggatgcgt
    32581 caccccgttg atacggtaac cgaaccagaa ccggctgaaa tcctggcaga gttcattcgc
    32641 cagcattctg cggctgggca gctggtggcg cgcgccgtgt ttctctcgcc accctattcg
    32701 gttgcagaag aggaactgtc agttttgctg gaaaacatta agcagaacgg agactacgcg
    32761 gatatcgctt gtatgacggg aagccaggat gattactatt actcaacgca ggccatgagt
    32821 gaaaactacg cagcgatgtc tttgcaagtt gtggagcagg acatttgtcg cgccattgca
    32881 cacgccgttc gttttgagtg ccagacgtat cctcgtcctt acatgtcaac gacggatgaa
    32941 aagtgatcca cttatatctc caccaacg
//
LOCUS       DAZFFE010000044        32896 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFFE010000044 DAZFFE010000000
VERSION     DAZFFE010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.044"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..76
                     /locus_tag="ACHJ69_002266"
     CDS             <1..76
                     /locus_tag="ACHJ69_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="rhodanese; catalyzes the formation of thiocyanate
                     from thiosulfate and hydrogen cyanide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO5015246.1"
                     /translation="SEMGYTHVENAGGLKDIAMPKVKG"
     gene            286..1966
                     /gene="ycjM"
                     /locus_tag="ACHJ69_002267"
                     /pseudo
     CDS             286..1966
                     /gene="ycjM"
                     /locus_tag="ACHJ69_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            1980..3272
                     /gene="ycjN"
                     /locus_tag="ACHJ69_002268"
     CDS             1980..3272
                     /gene="ycjN"
                     /locus_tag="ACHJ69_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5015247.1"
                     /translation="MIKSKIGLLSALVSCTLISGCKEENKNNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRQAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRNLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLISELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQTSQKKLDELVEQR"
     gene            3293..4174
                     /gene="ycjO"
                     /locus_tag="ACHJ69_002269"
     CDS             3293..4174
                     /gene="ycjO"
                     /locus_tag="ACHJ69_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO5015248.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            4161..5003
                     /gene="ycjP"
                     /locus_tag="ACHJ69_002270"
     CDS             4161..5003
                     /gene="ycjP"
                     /locus_tag="ACHJ69_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO5015249.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSEHFIKSGLTAG
                     GVKG"
     gene            5034..6086
                     /gene="ycjQ"
                     /locus_tag="ACHJ69_002271"
     CDS             5034..6086
                     /gene="ycjQ"
                     /locus_tag="ACHJ69_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO5015250.1"
                     /translation="MKKLVATAPRVAALVEYEDRAISANEVKIRVRFGAPKHGTEVVD
                     FRAASPFINDDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVNDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPITRRCDIARRHGADFCLNPIGADVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            6105..6893
                     /gene="ycjR"
                     /locus_tag="ACHJ69_002272"
     CDS             6105..6893
                     /gene="ycjR"
                     /locus_tag="ACHJ69_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO5015251.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQAAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHAHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAENPAQAYRDSLAWLRTC"
     gene            6903..7958
                     /gene="ycjS"
                     /locus_tag="ACHJ69_002273"
     CDS             6903..7958
                     /gene="ycjS"
                     /locus_tag="ACHJ69_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO5015252.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELEAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVTAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            7955..10179
                     /gene="ycjT"
                     /locus_tag="ACHJ69_002274"
                     /pseudo
     CDS             7955..10179
                     /gene="ycjT"
                     /locus_tag="ACHJ69_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
     gene            10176..10835
                     /gene="pgmB"
                     /locus_tag="ACHJ69_002275"
     CDS             10176..10835
                     /gene="pgmB"
                     /locus_tag="ACHJ69_002275"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO5015253.1"
                     /translation="MKLQGVIFDLDGVITDSAHLHFQAWQQIAAEIGISIDAQFNEFL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIHNLL
                     VDLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTESLTWPRLSAFWQN
                     V"
     gene            10849..11931
                     /locus_tag="ACHJ69_002276"
     CDS             10849..11931
                     /locus_tag="ACHJ69_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5015254.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIC"
     gene            11976..12881
                     /gene="ompG"
                     /locus_tag="ACHJ69_002277"
     CDS             11976..12881
                     /gene="ompG"
                     /locus_tag="ACHJ69_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO5015255.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETLALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(12932..13930)
                     /gene="ycjW"
                     /locus_tag="ACHJ69_002278"
     CDS             complement(12932..13930)
                     /gene="ycjW"
                     /locus_tag="ACHJ69_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5015256.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYEIPVVVIGKVEGQYSHV
                     YSVDTDNYGDSIALTNALIESGHKNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQRSCELLFQLIAGKPSPQNITVATHMSLK"
     gene            14086..15483
                     /gene="ycjX"
                     /locus_tag="ACHJ69_002279"
     CDS             14086..15483
                     /gene="ycjX"
                     /locus_tag="ACHJ69_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO5015257.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     SIHAGARLPLLSAVREERLLGVKRIPQRDFGITRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYFSWSRQMTDLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEEFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            15480..16541
                     /gene="ycjF"
                     /locus_tag="ACHJ69_002280"
     CDS             15480..16541
                     /gene="ycjF"
                     /locus_tag="ACHJ69_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO5015258.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            16689..18230
                     /gene="tyrR"
                     /locus_tag="ACHJ69_002281"
     CDS             16689..18230
                     /gene="tyrR"
                     /locus_tag="ACHJ69_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO5015259.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSTLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(18274..18780)
                     /gene="tpx"
                     /locus_tag="ACHJ69_002282"
     CDS             complement(18274..18780)
                     /gene="tpx"
                     /locus_tag="ACHJ69_002282"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO5015260.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            18899..19864
                     /gene="ycjG"
                     /locus_tag="ACHJ69_002283"
     CDS             18899..19864
                     /gene="ycjG"
                     /locus_tag="ACHJ69_002283"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO5015261.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVTTAQTIVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(19839..20567)
                     /gene="mpaA"
                     /locus_tag="ACHJ69_002284"
     CDS             complement(19839..20567)
                     /gene="mpaA"
                     /locus_tag="ACHJ69_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO5015262.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASHESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(20858..21496)
                     /locus_tag="ACHJ69_002285"
     CDS             complement(20858..21496)
                     /locus_tag="ACHJ69_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5015263.1"
                     /translation="MKNILILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(21567..22487)
                     /gene="ycjY"
                     /locus_tag="ACHJ69_002286"
     CDS             complement(21567..22487)
                     /gene="ycjY"
                     /locus_tag="ACHJ69_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5015264.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSDARTSDISSGEYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSQAGSKWMSDDLYDRASSKDKRYHIV
                     EGANHMDLYDGKVYVAEAISVLAPFFEETL"
     gene            22625..23524
                     /gene="pgrR"
                     /locus_tag="ACHJ69_002287"
     CDS             22625..23524
                     /gene="pgrR"
                     /locus_tag="ACHJ69_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO5015265.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            23861..25474
                     /gene="mppA"
                     /locus_tag="ACHJ69_002288"
     CDS             23861..25474
                     /gene="mppA"
                     /locus_tag="ACHJ69_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO5015266.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(25525..26556)
                     /gene="ynaI"
                     /locus_tag="ACHJ69_002289"
     CDS             complement(25525..26556)
                     /gene="ynaI"
                     /locus_tag="ACHJ69_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO5015267.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVTEIGWRITKIKTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPDQGR"
     gene            26800..27057
                     /gene="ynaJ"
                     /locus_tag="ACHJ69_002290"
     CDS             26800..27057
                     /gene="ynaJ"
                     /locus_tag="ACHJ69_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO5015268.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(27107..28057)
                     /gene="uspE"
                     /locus_tag="ACHJ69_002291"
     CDS             complement(27107..28057)
                     /gene="uspE"
                     /locus_tag="ACHJ69_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO5015269.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(28209..28961)
                     /gene="fnr"
                     /locus_tag="ACHJ69_002292"
     CDS             complement(28209..28961)
                     /gene="fnr"
                     /locus_tag="ACHJ69_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO5015270.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(29156..29671)
                     /gene="ogt"
                     /locus_tag="ACHJ69_002293"
     CDS             complement(29156..29671)
                     /gene="ogt"
                     /locus_tag="ACHJ69_002293"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO5015271.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKDGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGVTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(29682..29834)
                     /locus_tag="ACHJ69_002294"
                     /pseudo
     CDS             complement(29682..29834)
                     /locus_tag="ACHJ69_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(30184..30999)
                     /locus_tag="ACHJ69_002295"
     CDS             complement(30184..30999)
                     /locus_tag="ACHJ69_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5015272.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRIWAGESVWLVHASSALWLPPQMPHKFVATGNILLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            31096..32124
                     /locus_tag="ACHJ69_002296"
     CDS             31096..32124
                     /locus_tag="ACHJ69_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO5015273.1"
                     /translation="MIISKKQLIGVVAIGILLAGVVFFIWWVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLES
                     IQATIAMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIVFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            32121..32896
                     /locus_tag="ACHJ69_002297"
                     /pseudo
     CDS             32121..32896
                     /locus_tag="ACHJ69_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
BASE COUNT         8238 a         7722 c         8534 g         8402 t
ORIGIN      
        1 tagcgagatg ggatataccc acgttgagaa cgccggtggc ctgaaagaca tcgcaatgcc
       61 gaaggtcaaa ggttaaaaga attcaccatg agcggcgctt atgcgccgtt ttttctgtca
      121 caccctattt acatccccat agattatttg cgttagctca caaatacgct ttttcccagg
      181 taaaaaaaga tttcctgcgt gactaaaccc cttgagctca attgacagtt tattttatgc
      241 ggagtagtct ctcgtttcat gggaccgcta ccacggaaag gcaacatgaa acagaaaatt
      301 acggattacc tggacgaaat ctacggtgga acatttaccg cgactcattt acagaaactt
      361 gtaacgcgtc ttgagagtgc gaaacgatta attacacagc gacgtaaaaa acactgggat
      421 gaaagtgatg tcgtgttaat tacctatgcc gatcaatttc acagtaatga tttaaagcca
      481 ctacccacat ttaatcagtt ttaccctcaa tggctgcaaa gaattttttc acatgttcat
      541 ttattacctt tttatccatg gtcatctgat gatggatttt cggtaattga ttatcatcag
      601 gtcgccagtg aagcggggga gtggcaggat attcagcaac tcggtgaatg cagccattta
      661 atgtttgatt ttgtctgcaa ccatatgtcg gcaaaaagtg aatggtttaa aaactattta
      721 caacagcagc caggttttga agattttttt attgccgttg atccgaaaac cgatctcagc
      781 gccgtcactc gcccgcgtgc gttaccgtta ttaacgccat tccagatgcg cgatcattca
      841 atgcgccatt tatggaccac ctttagcgac gatcaaattg acctcaatta ccgtagccct
      901 gaagtgttgc tggcgatggt ggatgtattg ctgtgctacc tggaaaaggg cgcagagtat
      961 gtccgtctgg atgccgttgg ctttatgtgg aaagagccgg gaacaaactg catccatctg
     1021 gaaaaaacac atctgattat caaactgtta cggtcgatta ttgataacgt cgcgccaggc
     1081 acagtgatca ttaccgagac caatgttccg cacaaagaca acattgctta ctttggcaac
     1141 ggtgatgacg aaggcacata tggtgtacca gttctcgctg ccgccactgg tgctgcatgc
     1201 ggtgcaaaaa cagaacgttg aggcactttg tgcgtgggcg caaaacctga cactaccttc
     1261 cagcaacacc acctggttta acttccttgc ctctcacgat ggcatcgggc ttaacccact
     1321 gcgggggttg ttgcccgaaa acgaaatatt agcgctggtc gaggtgttac agcaggaagg
     1381 agcattagta aactggaaaa ataatcccga cggtacgcgt agcccctatg aaatgaatgt
     1441 cacctatatg gatgcgttga gccgccgtga gtgtagcgac gaagaacggt gtgccaggtt
     1501 tattctcgcc catgcgattt tgttaagttt ccccggtgtg ccagcgatat atattcaaag
     1561 cattctgggg tcgcgtaatg attacgcagg tgtcgaaaaa cttggatata atcgtgcgat
     1621 taaccgtaaa aaatactcta gtaaagagat aacgacagaa ctgaacaata aagcgacatt
     1681 aagttattcg gtatattata agttgtcgca tttaattaca cttcgtcgca gtcataaaga
     1741 gtttcatccg gataataatt tcaccattga tgcgattaat tcatttgtaa tgcgaattca
     1801 aagaagcaat gcggatggcg attgcctgac gggattgttt aatgtcagtg aaaatattca
     1861 gcatataaat attactgacc tgcacggtcg ggatctgatt agtgaagttg atatattggg
     1921 taatgaaata acactgcatc cctggcaggt tatgtggatt aaataaaaag gaacatttca
     1981 tgattaaatc aaaaatcggg ctgttatcag cactggtttc ctgcaccctg atttcaggct
     2041 gtaaagaaga aaataaaaat aatgtatcca tcgaatttat gcattcttcg gtggagcagg
     2101 agcgccaggc cgtcatcagc aaattgattg cccgttttga aaaagaaaac ccaggtatca
     2161 ccgtaaaaca agtgcccgtg gaagaagacg cctataacac caaagtcatt accctttcac
     2221 gtagcggttc gctgccggaa gtgatcgaaa ccagccatga ctacgccaaa gtgatggaca
     2281 aagagcagct tatcgatcgc caggcggttg ccacggtcat cagcaacgtc ggtgaaggcg
     2341 cgttctacga tggcgtactg cggattgtgc gcaccgaaga tggcagcgcc tggaccggtg
     2401 ttcctgtcag cgcatggatt ggcgggatct ggtatcgcaa agatgtgctg gcaaaagctg
     2461 ggctggagga gccaaaaaac tggcagcaat tgctggacgt tgcacagaaa ctgaatgacc
     2521 cggcgaataa aaaatacggt attgcgctgc ctacagcaga aagcgtgttg acggaacagt
     2581 ctttctccca gtttgcgtta tccaatcagg ctaacgtctt taacgccgaa ggcaaaatca
     2641 cccttgatac accagagatg atgcaggcgc tgacctatta ccgcaacctt gctgccaaca
     2701 ccatgccagg ttctaacgac atcatggaag tgaaagacgc ttttatgaac ggcaccgcgc
     2761 cgatggcgat ttactccacc tatatccttc cggctgtgat taaagaaggc gatccgaaaa
     2821 acgtcggttt cgtggtgcca acggagaaaa actctgcggt ctacggcatg ttgacctcgc
     2881 tgaccattac cgccgggcaa aaggccgaag agacggaagc ggctgaaaaa tttgtcacct
     2941 ttatggagca ggcagacaac attgccgact gggtgatgat gtcgccaggc gcggcgctgc
     3001 cggtgaacaa agcggttgtc actaccgcca cctggaaaga caacgacgtt attaaggcgc
     3061 tgggtgagct gccaaatcag ttaatcagcg aactgccaaa tattcaggtc tttggcgcag
     3121 taggggataa aaattttacc cgcatgggcg atgtgacggg ttctggcgtg gtgagctcca
     3181 tggtgcataa cgtcaccgtg ggtaaagccg acctccctgg cacgctgcaa acgagccaga
     3241 aaaagctgga tgagttggtt gaacagcgct aaacccagga caggaatccg caatgaacag
     3301 gcttttttca ggtcgttccg atatgccctt tgcgctgttg cttctcgcgc ccagcttatt
     3361 actgctgggc ggtctggtgg cgtggccgat ggtgtcgaat atcgaaatca gttttttacg
     3421 tctgccgctt aatcccaaca tcgagtcaac gtttgttggg gtgagcaact acgtgcgtat
     3481 tctctccgat cccggcttct ggcactcgct gtggatgacg gtctggtata ccgcgcttgt
     3541 ggtggcgggc agcaccgtgc tcgggctggc cgtggcgatg tttttcaacc gcgagttccg
     3601 cctgcgcaaa accgcgcgtt cgctggtgat cctctcctac gtaacgccgt ctatttcgct
     3661 ggtgttcgcc tggaaataca tgttcaacaa cggttacggc attgtgaact acctcggcgt
     3721 cgatcttctg catctctatg agcaggcacc gctgtggttc gataatccgg gcagtagctt
     3781 tgtgctggtg gtgctgttcg ccatctggcg ctacttcccg tatgccttta tttcgtttct
     3841 ggcgattttg cagaccatcg acaaatcgct gtacgaagcg gcagagatgg atggcgctaa
     3901 tgcctggcag cggtttcgta tcgtcacgct gcccgcgatt atgccggtcc tggcgacggt
     3961 ggtgaccctg cgcaccatct ggatgttcta catgttcgcg gatgtttatc tgctgaccac
     4021 caaggtcgat attctcggtg tctatctcta caaaaccgcc tttgccttta atgacttagg
     4081 gaaagcggcg gcgatctcgg tggtgctctt catcattatt ttcgctgtca ttctgctgac
     4141 cagaaaacgg gtgaacctca atggcaacaa ataaacgcac actcagtcgc atcggttttt
     4201 actgcgggct ggcgctgttt ctcatcatca cgctgtttcc attttttgtg atgctgatga
     4261 cctcgttcaa gagcgcaaaa gaggcgatct cgctgcatcc tacgctgctg ccacagcagt
     4321 ggacgctgga gcattacgtc gacattttta atccggtgat tttcccgttt gtcgactact
     4381 tccgtaacag catggtggtg tcggtggttt catccgtggt ggcggtattt ctcggcattc
     4441 tcggggctta cgcgctttcc cgcctgcgct ttaaagggcg gatgaccatc aacgccagct
     4501 tttacacggt gtacatgttc tccggcattt tgctggtggt gccgctgttc aaaatcatca
     4561 ccgcacttgg catttacgac accgagatgg cgctgattat caccatggtg acgcaaaccc
     4621 tgcccaccgc tgtgtttatg ctgaaaagct acttcgacac cattcctgat gagatcgaag
     4681 aagcggcgat gatggatggc ctcaatcgtc tgcaaattat cttccgtatc accgtgcccc
     4741 tggcgatgtc cgggctgata tcggtgtttg tctactgctt tatggtggcg tggaacgact
     4801 atctgtttgc gtcgattttc ctctccagcg ccagcaattt caccttacca gtgggcctga
     4861 acgcgctgtt cagtacgcct gattacatct ggggacggat gatggcggcc tcgctggtga
     4921 ccgcattgcc ggtggtgatt atgtatgcgc tttcggaaca ttttattaag agtggtttga
     4981 ccgccggtgg cgtgaagggc taaagcggcc agtttcttga caaggagttt tacatgaaaa
     5041 agttagtagc cacagcaccg cgcgttgctg cgctggttga gtatgaagat cgggcgattt
     5101 cagccaatga agtgaagatc cgcgtgcgat tcggcgcgcc gaaacacggg acggaagtgg
     5161 ttgatttccg cgccgccagc ccgtttatta atgacgactt taatggcgaa tggcagatgt
     5221 tcaccccgcg tccggcagat gcgccgcgcg gcattgagtt tggcaagttc cagcttggca
     5281 acatggtggt tggcgacatt atcgagtgcg gcagcgacgt taacgactac gcggtgggcg
     5341 acagcgtatg cggctacggc ccgctctccg agacggtcat cattaacgca gtgaataact
     5401 acaagctgcg caaaatgccg caaggcagct cctggaaaaa cgccgtctgc tacgacccgg
     5461 cgcagtttgc catgagcggc gtgcgcgatg ccaacgtacg cgtgggcgat tttgtggtgg
     5521 tggtcgggct tggcgcgatt ggtcaaattg ccattcaact ggcaaaacgc gctggcgcgt
     5581 cggtggtgat tggcgtcgat cctattaccc gtcgttgtga tattgcccgt cgccacggtg
     5641 cggatttctg tcttaacccc attggcgctg acgtaggtaa agagatcaaa acgctgaccg
     5701 gcaagcaggg tgccgatgtg attatcgaaa ccagcggtta cgccgacgcg ctgcaatcgg
     5761 cgctccgcgg tctggcctac ggcggcacca tctcctatgt cgcgtttgcc aaaccatttg
     5821 ctgaaggttt taacctcgga cgcgaagcgc atttcaataa cgccaaaatt gttttctccc
     5881 gcgcgtgcag cgaaccgaac ccggattatc cgcgctggag ccgtaagcgt attgaagaaa
     5941 cctgctggga actgctgatg aacggttatc tcaattgcga agatttaatc gatccggtgg
     6001 tgacctttgc caacagcccg gaaagctaca tgcagtatgt cgaccagcat ccggaacaga
     6061 gcatcaaaat gggcgtcacg ttttaattca aaggaacgaa aagaatgaaa atcggcacac
     6121 agaatcaggc gttctttccg gaaaacattc tggagaaatt tcgttatatc aaagagatgg
     6181 gtttcgatgg ttttgagatt gacggcaaac tgctggtcaa caatctcgaa gaagtcaaag
     6241 cagcaatcaa agaaaccggt ttaccggtga ccaccgcctg cggtggctat gacggttgga
     6301 ttggcgactt tatcgaagag cgtcgactta atggcttaaa gcagatcgaa cgcattcttg
     6361 aagcgctggc agaagtgggc ggtaaaggta tcgtcgttcc ggctgcatgg ggcatgttta
     6421 ccttccgctt accgccgatg acctcgccgc gtagcctgga cggcgaccgc aaaatggtga
     6481 gtgattccct gcgcgtactg gaacaggcgg ccgcacgtac cggaaccgtg gtgtacctcg
     6541 aaccgttaaa ccgctatcag gatcacatga tcaacaccct cgccgatgct cgtcgttaca
     6601 tcgtcgaaaa cgatcttaaa catgtacaga ttatcggcga tttctatcac atgaatatcg
     6661 aagaagataa cctggcgcag gcgttgcacg acaaccgcga cttgctcggt catgcgcata
     6721 ttgccgataa ccatcgctac caaccgggca gcggcaccct cgatttccac gcgctgtttg
     6781 aacagctgcg cgccgataac tatcagggct atgtggtgta tgaagggcgt atccgggcag
     6841 aaaatcctgc ccaggcgtac cgtgattcgt tggcctggtt gcgcacctgc taagaggtct
     6901 ttgtgaaaag tgcaatgaca agctctccgc tgcgagtcgc gatcataggc gcgggccagg
     6961 tggcggataa agttcatgct tcgtactact gcacccgcaa cgatctggaa ctggaggctg
     7021 tctgtgacag ccgcctttcc caggcgcagg cgctggcaga aaaatacggg aatgcatccg
     7081 tgtgggacga tccgcaggcc atgctgctgg cggtgaaacc tgatgtggtt agcgtctgct
     7141 cacctaaccg ttttcattac gaacataccc tgatggcgct ggaagcgggc tgccatgtga
     7201 tgtgcgaaaa accgcccgcc atgacgccag aacaggcgcg ggaaatgtgc gataccgcgc
     7261 gcaaacaggg caaggtgctg gcctacgact ttcaccatcg ttttgcactc gatacgcaac
     7321 agctgcgtga acaggtgacc aacggcgttt tgggagagat ttacgttacc accgcccgcg
     7381 ccctgcgtcg ctgcggtgtt cccggctggg gtgtctttac taataaagaa ttgcagggcg
     7441 gcggcccgct gatcgacatc ggcattcata tgctggatgc ggcgatgtat gtgctgggtt
     7501 ttccagcggt gaaaagcgtg actgcgcata gctttcaaaa gatcggcacg caaaagagct
     7561 gcggtcaatt tggcgagtgg gacccggcaa cctacagcgt cgaagattcg ctgtttggca
     7621 ccattgaatt tcataacggc ggcattctgt ggcttgagac ctcatttgca ctcaacatcc
     7681 gcgaacagtc gattatgaac gtcagctttt gtggtgataa agccggggcg acgctgtttc
     7741 cagcacatat ctacaccgat aacaacggtg agttaatgac gctgatgcaa cgggaaatgg
     7801 cagacgacaa ccgtcatctg cgcagtatgg aagcctttat caaccacgta caaggtaagc
     7861 ccgtgatgat agccgacgcc gagcaggggt acatcatcca gcaactggtg gcggcgttgt
     7921 atcaatccgc agaaacaggg acgcgtgtgg aattatgacc aggccagtaa cgttaacgga
     7981 accccatttc agccagcata ccctgaacaa gtatgcttcg ctgatggcgc aggggaacgg
     8041 ctatcttggg cttcgtgcca gccatgaaga agattacacg cgccagaccc gcggcatgta
     8101 tctggcaggg ctttatcatc gagctgggaa aggtgaaatc aacgaactgg tgaacctgcc
     8161 tgatgtcgtg gggatggaga ttaccttgaa tggtgaactt tttgcgctat cccgcgaaac
     8221 gtggcagcgc gagcttgact tcgccagtgg agaattacgt cgcaatgtcg tctggtcttc
     8281 agccagcggc gcacgttatg ccattgccag ccgtcgcttt gtttcggcag aacaactgcc
     8341 gctgatggcg ctggaaatca gcattacgcc gctggacgct gacgcgtcag tgctgatttc
     8401 aacaggcatt gacgccacgc aaaccaacca cggacgacaa catctcgacg aaacccaggt
     8461 gcgggtgttt ggtcagcatt tgatgcaggg gatctatacc actcaggatg gacgcagtga
     8521 tgtggccatc agctgttgct gccaggtgag cggtgacgtg cagcaatgct ataccgccaa
     8581 agagcgccgc ttgctgcaac ataccagtgc acaactacct gcgggcaaaa cgctgacgct
     8641 gcaaaaacgg gtgtggatcg actggcggga cgacagacaa gccgctttag acgaatgggg
     8701 cagtgcgtcg cttcgtcagc ttgaaatgtg cgcgcagcag agttacgacc aacttcttgc
     8761 agcatcaaca gaaaactggc gtcaatggtg gcagaaacgt cgtatcacgg taaacggcgg
     8821 cgatgcgcac gatcagcaag cgttagatta tgcgctttat cacctgcgca tcatgacgcc
     8881 tgcccacgat gagcgcagca gcattgcggc gaaaggttta accggcgaag gctacaaagg
     8941 ccacgttttc tgggatacag aagtattttt gctaccgttc catctgttta gcgatccgac
     9001 ggttgcccga agtttactgc gttatcgctg gcacaacttg cctggcgcgc aggagaaagc
     9061 gcgcgtagcg gcgaagaaga gacgccggaa tttgctgcca ttaacattcg taccgggttg
     9121 cggcaaaaag tggcctctgc gcaggcggaa catcatctgg tggccgatat cgcctgggcg
     9181 gttattcaat actggcggac cacgggggat gaaagtttca ttgctcatga aggcatggcg
     9241 ctacttctgg agacggcaaa gttctggatt agccgcgcgg tgagggttaa cgaccgtctg
     9301 gaaattcatg atgttattgg gccagatgaa tataccgaac atgtcaataa taacgccttc
     9361 accagctata tggcgtatta caacgtccag caggcgctga atatcgctcg tcagtttggc
     9421 tgtagcgacg acgcgtttat tcatcgtgca gaaatgtacc tcaaagaact gctgctgcca
     9481 gaaattcagc ccgacggcgt tttgccgcag gatgattcgt ttatggctaa gccggtgatt
     9541 aatctggcga aatacaaagc ggcggcgggg aagcaaacca ttctgctgga ttattcacgc
     9601 gcagaagtga acgagatgca gatcctcaaa caagctgatg tggtgatgct caattacatg
     9661 ctgccggagc agttctcagc ggcatcgtgt cttgccaatc tgcaatttta tgaaccgcgc
     9721 actattcacg actcgtcatt aagtaaagca atccacggca ttgttgccgc acgctgtggc
     9781 ctgctgaccc aaagttatca gttctggcgc gaggggactg aaatcgatct tggtgctgat
     9841 ccacatagct gtgatgacgg tatccacgct gccgcaactg gcgctatctg gctgggggcg
     9901 attcagggtt ttgccggggt gagcgtgcgt gacggtgaat tacatctcaa tccggcgtta
     9961 cctgagcagt ggcaacagtt gtctttccct ctgttctggc agggctgcga attacaggtc
    10021 actctcgacg cgcagcgtat tgcgattcga acttctgcgc ccgtttcact gcgcttgaac
    10081 ggtcagctta tttacgtcgc tgaagaatct gttttctgtt tgggggattt tattttgccc
    10141 ttcaatggga tcgctaccac gcatcaggag ggtgaatgaa actgcaaggg gtaattttcg
    10201 atctggatgg agtaatcaca gatagcgcac atctgcattt ccaggcgtgg cagcagattg
    10261 ccgctgaaat tggcatcagc attgatgcgc agtttaacga attcctaaaa gggatcagcc
    10321 gcgatgagtc cctgcggcgg attctgcagc acgggggtaa agagggcgat tttaacccac
    10381 aggaacgggc gcaactggcg tatcgcaaaa atctgcttta cgtccactca ctgcgcgaac
    10441 tgacggttaa tgctgtgctg cctggcattc acaatttgct ggtagatctc cgagcacagc
    10501 agattccggt tgggctggct tctgtctccc tgaatgcgcc gacgatttta gcagcgctgg
    10561 agctgcgtga gtttttcacc ttctgcgcgg acgcttccca acttaaaaac tcgaaaccgg
    10621 acccggaaat ctttctcgcc gcctgtgccg gactgggcgt gccgccgcag gcatgtatcg
    10681 gcattgaaga tgcgcaggcg ggcattgacg ccattaacgc cagcggtatg cgctcggtgg
    10741 ggatcggcgc aggcttaacc ggggcgcaat tattgttgcc ctcaacggaa tcactcacct
    10801 ggccgcggtt atcggccttc tggcaaaacg tatagcaaag gaatcaacat ggctcagctt
    10861 tcgttacaac atattcaaaa gatctacgat aaccaggtgc atgtggtgaa ggacttcaac
    10921 ctggagattg ccgataaaga gttcatcgtg tttgtcggtc cgtcgggctg cggtaaatcg
    10981 accaccctgc gtatgattgc cgggcttgag gagatcagcg gtggtgatct gttgatcgac
    11041 ggcaaacgaa tgaatgacgt tccagccaaa gcacgcaata tcgcgatggt gttccagaac
    11101 tacgcgctgt atccgcatat gacggtctac gacaacatgg cgtttggcct gaagatgcaa
    11161 aaaatcgcca aagaggtgat tgatgagcgg gtgaactggg cggcgcaaat tctcggcctg
    11221 cgtgagtacc tgaaacgcaa gccgggcgcg ctttccggcg ggcaacgtca gcgagtggcg
    11281 cttgggcggg cgattgtgcg cgaagcgggc gtgtttttaa tggatgagcc gctctctaac
    11341 ctagatgcca agctgcgcgt gcaaatgcgc gcagagatca gcaagctgca tcagaaactg
    11401 aacaccacca tgatctacgt gacccacgat cagaccgaag cgatgaccat ggcgacgcgg
    11461 attgtcatta tgaaggatgg gattgttcag caggtcggtg cgccgaaaac ggtttataac
    11521 caacctgcga atatgtttgt tgccggattt attggatcac cagcgatgaa ttttattcgc
    11581 ggcacgatcg atggcgataa attcgttacg gaaacgctta aattaaccat acccgaagag
    11641 aaattagcgg ttctgaaaac acaggaaagt ttgcataagc ccatcgtaat gggaatacgc
    11701 ccggaagata ttcatccgga cgcgcaagag gaaaataaca tttccgccaa aattagcgtg
    11761 gcggaattaa ccggtgcgga atttatgctc tacaccacgg ttggggggca cgagttagtg
    11821 gtccgtgctg gtgcgttaaa tgattatcat gcaggagaaa atatcactat tcattttgat
    11881 atgactaaat gtcatttctt tgatgcagaa acggaaatag caatttgcta aatacagggg
    11941 gaaggcattc tcccaggata atacaaggaa caataatgaa aaagttatta ccctgtactg
    12001 cactggtgat gtgtgcggga atggcctgcg cacaggccga ggaaaagaac gactggcact
    12061 ttaatatcgg cgcgatgtac gaaatagaaa acgtcgaggg ttatggcgaa gatatggatg
    12121 ggctggcgga gccttctgtc tattttaatg ccgccaacgg gccgtggaga atttctctgg
    12181 cctattatca ggaagggccg gtagattata gcgcgggtaa acgtggaacg tggtttgatc
    12241 gcccggagct ggaggtacat tatcagttcc tcgaaagcga tgatttcagt ttcggtctga
    12301 ctggtggttt ccgtaattat ggttatcact acgttgatga accgggtaaa gacacggcga
    12361 atatgcagcg ctggaaaatt gcgccagact gggatgtgaa actgactgac gatttacgtt
    12421 tcaacggttg gctgtcgatg tataaatttg ccaacgatct gaatacgacc ggttatgctg
    12481 atacccgtgt cgaaacggaa acaggtctgc aatatacctt caacgaaacg cttgccttgc
    12541 gagtgaacta ttatctcgag cgtggtttca atatggatga tagccgcaat aacggtgagt
    12601 tttccacgca agaaattcgc gcctatttgc cgctgacgct cggcaaccac tcggtgactc
    12661 cgtatacgcg cattgggctg gatcgctgga gtaactggga ctggcaggat gatattgaac
    12721 gtgaaggcca tgattttaac cgtgtgggtt tattttacgg ttatgatttc cagaatggtc
    12781 tttccgtttc gctggaatac gcgtttgagt ggcaggatca cgacgaaggc gacagcgata
    12841 aattccatta tgcgggtgtt ggggtgaatt actcgttctg ataatgtgct aaattgccgg
    12901 atgcggggcg agcgccaaat ccggcataca tttatttcaa cgacatatgc gtagcaacgg
    12961 taatattttg tggtgacggt tttccggcaa ttaactggaa taataactcg cagcttcgtt
    13021 gacctaactc ctgcgtcgga acatcgatgc cgcctggtgc aggcgttaat ataaacgaca
    13081 gcgtttcatt actataaccc accaccgcta actgctgcgg aatagcaatg tttttctctg
    13141 ccgccgcacg ataaatgctc attaatttca ggctgtcagt ggcaaatacg gcttctggca
    13201 acggcgactg gcttaataat tgccgtgcgg cttgcaaggc tgtttcatgg gtataaccgc
    13261 cgtcaacaat ccattcatca cgcactgcaa tattatgcgc agccaggctt tgcttataac
    13321 catttacccg atccactgaa acatgtacat caagcggcgc atgcaggcag gctatattct
    13381 tatgcccgct ttcaattaac gcattggtca gcgcaatact gtcgccataa ttatcggtat
    13441 cgacggaata aacatggctg tattgacctt caactttacc gatcaccacc accgggatct
    13501 cgtatttgtc gagttgtgca aagaaggatt catcggcagg cgaacttagc ataataatgc
    13561 ctttaatcat tttctgctta attttgcttt cgcatttttg cagatcttct gccggattgt
    13621 gcgacgtttg taatatcacg tcgaaacctt cttcttctgc tttggcggtg atggcatgca
    13681 aaacttccga gaaaaatgga ttacccgccg tagttttggt cgaacgggta gaaatcacca
    13741 taatggcatc aaaacccgaa gaggtcagcg cgcgggccag cttgtttggt tgatactgta
    13801 attcttcaat ggcccgtaac actttttcgc gcgcttccgg ggagatattg gtttgcttat
    13861 tcagcacgcg tgatacggtg gattttgata cgcctgcaac cctggcaata tcataaatag
    13921 taggggacat aggtcaggaa ctccgtccga acgtgaatgc tgcacatctt atggagttcg
    13981 tccgtcgatg acaacacggg tagttgataa tcaatggcct ggcccccaca ttcatatcct
    14041 tacgaacgat tttttttctg gtcttcagag cagcacagga cagcaatgaa gcgacttaaa
    14101 aatgaactca atgcgctggt gaatcggggt gtcgacagac atctgcgcct cgctgtaacc
    14161 ggacttagcc gcagcggcaa aacagcgttt atcaccgcga tggttaatca gttgctcagt
    14221 attcatgccg gagcacgttt gccgctatta agtgcggtgc gtgaagagcg cctgctgggc
    14281 gtaaaacgca ttccccagcg tgactttggc attacgcgct tcacctatga tgaagggttg
    14341 gcgcagttat atggcgatcc tcccgcctgg ccgacgccta cgcgcggcgt cagtgaaatt
    14401 cgcctggcgt tacgttttaa atcgaacgat tcgctgctgc gccactttaa ggacacctcc
    14461 acgctgtatc tggagattgt ggattatccc ggcgaatggc tgctcgacct gccgatgctg
    14521 gcacaggact attttagctg gtcgcgccag atgacggact tactcaatgg tcagcgcggc
    14581 gaatggtcgg cgaaatggcg aatgatgtgt gaaggtctgg acccgttagc gcctgccgac
    14641 gaaaatcgcc tggcggatat tgccgctgca tggaccgatt atctccacca ctgcaaacag
    14701 caggggctgc actttattca gccaggacgc tttgttttgc ctggcgatat ggcaggagcg
    14761 cccgcgctgc aattcttccc atggccagat gtcgatgcct ggggcgagtc caaactggcg
    14821 caggccgata aacacaccaa cgccggaatg ttgcgcgagc gttttaatta ttactgcgag
    14881 aaagtggtga aggggttcta taagaatcac ttcctgcgtt ttgaccgcca gattgtgctg
    14941 gtggactgcc tgcaaccgct caacagtgga ccacaggcat ttaatgatat gcgtctggcg
    15001 ctgacgcagc tgatgcaaag tttccactac gggcagcgta ccctgttccg gcgtttgttt
    15061 tcgccggtta tcgataagct attgtttgct gccactaaag cggaccatgt gaccatcgat
    15121 cagcacgcta atatggtttc attgctgcaa caactgattc aggatgcctg gcaaaatgcg
    15181 gcgttcgaag ggatcagtat ggactgcctg gggctggcgt ctgttcaggc gaccaccagc
    15241 ggaattattg atgttaacgg tgagaaaatc ccggcgctgc gcggtaatcg actcagcgat
    15301 ggcgcaccgc tcactgttta tcctggcgaa gttcccgcac gtttgccagg tcaggcgttc
    15361 tgggataagc aaggcttcca gtttgaggag tttcgtccgc aggtgatgga tgtcgacaaa
    15421 ccgctaccgc atattcgtct tgatgctgcg ctggaatttt taataggaga taaattgcga
    15481 tgaccgaacc gttaaaacca cgtattgatt tcgacggtcc actggaggtc gatcagaatc
    15541 caaaattcag ggcgcagcag acctttgacg aaaatcaggc gcaaaatttt gccccagcca
    15601 cgctcgatga agcgcaggaa gaagaggggc aagttgaagc ggtgatggac gcagcgttgc
    15661 gtccgaaacg cagcctgtgg cgcaaaatgg tgatgggcgg gctggctctg tttggcgcaa
    15721 gcgttgtcgg gcagggcgtg cagtggacaa tgaacgcctg gcaaacccag gactgggtgg
    15781 cgctgggtgg atgtgccgct ggggcattga ttatcggcgc tggcgtaggt tctgtggtaa
    15841 cagagtggcg gcgcttatgg cgcttgcgac agcgcgccca tgaacgcgac gaagcgcgtg
    15901 atttattgca cagccatggc accggcaaag gccgcgcatt ttgcgaaaaa ctggcgcagc
    15961 aggcgggcat cgatcagtca catccggcgc tgcaacgctg gtatgcctca atccatgaaa
    16021 cgcaaaacga ccgtgaagtg gtcagtttgt atgcgcattt ggtccagcca gttttagatg
    16081 cccaggcgcg gcgcgaaatc agccgttcgg cagcggaatc aacgttaatg attgccgtta
    16141 gcccactggc gctggtggat atggcgttta tcgcctggcg caatctgcgt ttgattaatc
    16201 gcatcgccac gctgtatggc attgaactgg gatattacag ccgtttgcgc ctgtttaagc
    16261 tggtattgct gaatattgct tttgccggag ccagcgaact ggtgcgcgaa gtggggatgg
    16321 actggatgtc gcaagatctc gctgcgcgtt tgtctacccg ggcagctcag gggattggtg
    16381 ctggacttct gacggcacga ctggggatta aagctatgga gctttgccgc ccgctgccgt
    16441 ggattgacga tgacaaacct cgcctcgggg atttccgtcg tcagcttatc ggtcaggtga
    16501 aagagacgct gcaaaaaggc aaaacgccca gcgaaaaata atgcaatatc gggtgctgac
    16561 cggatatctt tacgccgaag tgcccgtttt tccgtctttg tgtcaatgat tgttgacaga
    16621 aaccttcctg ctatccaaat agtgtcatat catcatatta attgttcttt tttcaggtga
    16681 aggttcccat gcgtctggaa gtcttttgtg aagaccgact cggtctgacc cgcgaattac
    16741 tcgatctact cgtgctaaga ggcattgatt tacgcggtat tgagattgac cccattgggc
    16801 gaatctacct caattttgct gaactggagt ttgagagttt cagcactctg atggccgaaa
    16861 tacgccgtat tgcaggtgtt accgatgtgc gtactgtccc gtggatgcct tccgaacgtg
    16921 agcatctggc gttgagcgcg ttactggagg cgttgcctga acctgtgctc tctgtcgata
    16981 tgaaaagcaa agtggatatg gcgaacccgg cgagctgtca gctttttggg caaaaattgg
    17041 atcgcctgcg caaccatacc gccgcgcaat tgattaacgg ctttaatttt ttacgctggc
    17101 tggaaagcga accgcaagat tcgcataatg agcatgtcgt tattaatggg cagaatttcc
    17161 tgatggagat tacgcctgtt tatcttcagg atgaaaatga tcaacacgtc ctgaccggtg
    17221 cggtggtgat gctgcgatca acgattcgta tgggccgtca gttgcaaaat gtcgccgccc
    17281 aggatgtcag cgccttcagt caaattgtcg ctgtcagccc gaaaatgaag catgttgtcg
    17341 aacaggcgca gaaactggcg atgctaagcg cgccgctgct gattacgggt gataccggca
    17401 ccggaaaaga tctctttgcg tatgcctgtc atcaggcaag ccccagagcg agcaaacctt
    17461 acctggcgct gaactgtgcg tctataccgg aagatgcggt cgagagtgaa ctgtttggtc
    17521 atgctccgga agggaagaaa gggttctttg agcaggcgaa cggtggttcg gtgctgttgg
    17581 atgaaatagg tgagatgtca ccacggatgc aggcgaaatt actgcgtttc cttaatgatg
    17641 gcaccttccg tcgggttggc gaagaccatg aggtgcatgt cgatgtgcgg gtgatttgtg
    17701 ctacgcagaa gaatctggtc gaactggtgc aaaaaggcgt gttccgtgaa gatctctatt
    17761 atcgtctgaa cgtgttgacg ctcaatctgc cgccgctacg tgactgtccg caggacatca
    17821 tgccgttaac cgagctgttc gtcgcccgct ttgccgacga gcagggcgtg ccgcgtccga
    17881 aactggccgc tgatctgaat actgtactta cgcgttatgc gtggccggga aatgtgcggc
    17941 agttaaagaa cgctatctat cgcgcactga cacaactgga cggttatgag ctgcgtccac
    18001 aggatatttt gttgccggat tatgacgccg caacggtagc cgtgggcgaa gatgcgatgg
    18061 aaggttcgct ggacgaaatc accagccgtt ttgaacgctc ggtattaacc cagctttatc
    18121 gcaattatcc cagcacgcgc aaactggcaa aacgtctcgg cgtttcacat accgcgattg
    18181 ccaataagtt gcgggaatat ggtctgagtc agaagaagaa cgaagagtaa gcgtgaatat
    18241 gcctgatggt gcaacaccat caggcatatt aacttatgct ttcagtacag ccagagctgc
    18301 ttcgtaatcc ggctcggtgg tgatttcatc caccagctgg ctgaaaatca cattgtcatt
    18361 ttcatcaata accacaacgg cacgcgctgc cagacctttc agtgggccat cagcaattgc
    18421 cacaccgtag gcttgcagga attcagcgtt acggaaagtg gagagggtga taacgttgtt
    18481 cagaccttct gcgccgcaga aacgagactg ggcgaacggc agatcggcag agatacacag
    18541 cacaacggtg ttgtcgatct cggttgccag ttggttaaac ttacgtactg atgcggcgca
    18601 aacaccggta tcaatactcg ggaaaatgtt cagcactttg cgtttacccg caaactgacc
    18661 gagggtgacg tcagacagat cttttgccac gagagtaaaa gtctgcgctt tgctacccgc
    18721 ctgcgggatg gaattggcga ctgtaaccgg gttgccctgg aaatgaacgg tttgtgacat
    18781 gattatcttt cctgtttaca tatagttaac gtcacaccta gtttatgcta actgtcaata
    18841 acacagcaaa cgctatttgc gcttaatccg cagaccaccg cgacaacaag gagtaaagat
    18901 gagaaccgtt aaggtatttg aggaggcctg gcccttacat accccgtttg tgattgctcg
    18961 gggaagtcgc agtgaagcgc gcgtggtggt ggttgaactg gaagaagagg gtattaaagg
    19021 caccggcgaa tgcacgccgt atccgcgtta tggggaaagt gatgcctcgg taatggcgca
    19081 aattatgagc gtcgtgccgc aattagagaa agggctgaca cgggaggagt tgcaaaaaat
    19141 cctccctgcc ggcgcagcac gtaatgcgct ggattgtgca ttgtgggatc tggcggcgcg
    19201 aaaacagcag caatcgctgg ctgatttgat cggcataacg cttcccgaga cagtcaccac
    19261 ggcacagacg atagtcatcg gtacgcctga tcagatggcc aatagtgcat caacactctg
    19321 gcaggcaggc gcgaaattac tgaaagtgaa gctggataac catcttatca gtgagcggat
    19381 ggtggcaatt cgcacagctg tgcccgatgc gacgctgatc gttgatgcaa atgaatcctg
    19441 gcgtgcagaa gggttggcgg cgcgttgcca gctattggca gatttaggcg ttgcgatgct
    19501 tgaacaaccg cttcctgcgc aggacgatgc ggcgctggag aattttattc atccgttacc
    19561 gatttgtgct gatgaaagtt gtcatactcg tagcaatctg aaggcgctga aagggcgcta
    19621 tgagatggtt aacattaagc ttgataaaac cggtggtctg acggaagcgc tggcgctggc
    19681 gactgaagcg cgtgcacaag gttttcgtct gatgctgggc tgcatgttgt gtacctctcg
    19741 tgccattagc gccgctttac cgctggtgcc gcaggtcagt ttcgccgatc ttgacggacc
    19801 gacctggctg gcggtagatg tggaaccggc gcttcagttc acgacgggcg aattgcatct
    19861 ttaggatgcc agcgcagcaa gtttgccatc gcaaagagat atttttcgct ggcttcgtcg
    19921 gaggagatgg gtggaaactc tgcggtgatg caatgcaagt tgagatctgc acaccaactg
    19981 ccgaaagagc caggcgtttc ataaccgacg ctggttacca gaggcaattc aaacgcctgg
    20041 gccagccatt cacctaattc gctgtgtctg ggatcttcaa tacaggccag cggatcgtgg
    20101 aatgagacca cccaggcagg ttgaatgcgg tgtataagct ggcacagcgc ctgggtttca
    20161 ggttcggagc caggtttgtc acctgtcagt aaaacgacat cacgttcttc agcggcgctg
    20221 ttccagcgat aaactgtttc accttccttc cagttcgctg ccggaaagtt tcggtttaaa
    20281 tccacaccat tagcattggc ccgtaaccct aactggcagc cgtcaggatt cacacacagc
    20341 accacatgat ggcggcgcaa agaaggtgtc aaagtccgca gagcacagga gagcgtgacg
    20401 atcgaagagt tttcatcacc gtgagtgcca gccagaatca aaccactttc gtgactggct
    20461 gcaggggccg gaaaccagat taacggcgcg cccaataatg aacgtccgta atgttctgtt
    20521 ccgggcggaa atgcgccgcg ttcggcgcgt gggcgagtta cggtcatgat cgtctctggt
    20581 tcaggaagta ttacaggcag tgttgtgcaa attttgcgga ttatcaaatc cttttcttca
    20641 acgctatgtc atcgatcttg tatcactttt ttgccggaag ttgtttgtat tttcattgat
    20701 gtgcagatat tagaattgtc gtaatgttat atacatggcg tattttattc ttactggttc
    20761 tacacatgtt aatcgcatct gattgatcag ttcaccttaa taagtttaat aaatcgtact
    20821 gcacatagta cagtacgatg gtagctatat aattgattta catgaagaag ggcttatcac
    20881 catcagtccc gggttgattg atgcctatat tctcaccaat gtgtttctca ggtttatcga
    20941 tgatatctgt gattaatgct gcgacactct tacgtgatac gatcgtacct ttgaatggtt
    21001 cattgcgtga ggtaagctca taatcaataa tatcttcatc agtcagccag gcagggcgaa
    21061 gaattgtata ttccagtcca gaagcctcaa ttgcatccgc agccctgcgg aatggtttta
    21121 ggggttcacc aataactgca ttattccatt cgccaaattt tcctggaact tcatcataaa
    21181 taccgaggga aagaacaaag atcagacgtt taacatcaca ggctttcatc gcagcgatca
    21241 ctgaatttgc ctgaatatct aaatcttccc ccgtaaggtt ggcatacaca acatcctgtc
    21301 cctgcatggc ctgcttcagt gctgcatgat tcagtacatc ccccataata atcttgctat
    21361 tagtgggata gggtttgtgg atttttgctg gctgacgggc aaataaagtt tgtttaatgg
    21421 tttgtttatc tgccagttga ttaatcacat ggcgggctat ctgaccgcca gcaccaagaa
    21481 tcagtatatt tttcatgaaa cttttcccag attattggca gcaatatgcc aggccatatc
    21541 actattacgg atttgaatat gtttcattac agagtttcct caaagaacgg agctaatact
    21601 gaaattgctt cagccacata gactttgccg tcataaagat ccatatggtt tgcgccttca
    21661 acaatgtgat agcgtttatc cttgcttgat gctcgatcgt acaggtcgtc actcatccat
    21721 ttgctcccag cctggctgcc cgccacaatc tgtatcggct gagtcaggta cacttccgcc
    21781 atatggtaag catcataggt aataatctgg ttaaggctgc gcaaagtagc gtaacccggt
    21841 gctgttggat actgcgcgcg aggggtgtgg taatactccc aggcctgacg cagttcttcg
    21901 ttcggcgcat cggactcctt cattggtgcc agtggcatag tggcgtattc tccgctgcta
    21961 atatcgctgg ttcgggcgtc tgaacccgcg tcaacgtatg gtagggcatc aatagatttc
    22021 acattgtttt cccaaccatt acggaacatc gaaccaatat tgaccgcact aacagtaccg
    22081 atggccttga tgcggcgatc ctgaattgca gcattggctg tatatcctgc tccggcacaa
    22141 attcccatcg caccaattcg ggtattgtcg acataagaaa gcgttgtcag gtaatcaatc
    22201 acggcactga cgtcttcagt acgaatgtat gggttttcta actgacgcgg ctcgccgccg
    22261 ctttcacctt gataagatgc gtcataagca atagtgacaa atcccttttc cgccagtttt
    22321 tcggcatagg ttccggccgt ttgctcttta acgcccccac ctgggtgaga taccacaatt
    22381 gccggatact gacgggtttc atcaaatttt ggagggaaat agatcactgc agacaaagag
    22441 atggttggat tattgctgtt agtgaagctg actttattat tcatcattcc gttcctcatg
    22501 gagttgtcgg ttcgttttaa cggttggtga tatcactata gatattgatc attaagttga
    22561 ttagacccaa aatcatgatt agacctatca tttaaatgat taatagattg tatggagata
    22621 ctgaatgaag cgtgaagaaa tcgctgatct gatggcgttt gtcgttgttg cagaggagcg
    22681 tagcttcact cgcgcagcag cccgcctgag catggcgcag tcagctttaa gccagatagt
    22741 gcgccgtata gaagaacgat tgggattgcg gcttctgacg cgaaccacgc gcagcgttgt
    22801 acccactgaa gcgggcgagc atcttttgtc tgttcttggc cctatgttgc atgacataga
    22861 ttctgccctg acatccctga gcgatctgca gaaccgccca tccgggacaa tacgtattac
    22921 tactgtagaa catgcagcaa aaacgatatt gttaccagct atgcgcacat tcctgaaatc
    22981 gcatcctgaa attgatattc agcttaccat tgattatggt ttgaccgatg tcgtttctga
    23041 acgttttgat gcaggcgtcc gtctgggtgg ggagatggat aaagatatga tcgccattcg
    23101 aatcgggcca gacataccaa tggctattgt tggctcaccg gattattttt ctcgtcgaag
    23161 tgctccaacg tcagtgtcac aattaataga tcatcaggca attaatttgt atcttcccac
    23221 atcgggtaca gcaaatcgct ggagattaat acgcggtgga cgtgaagttc gtgttcgcat
    23281 ggaaggtcag cttttactga atacgataga cctgatcatt gatgctgcaa ttgatgggca
    23341 tggattggcg tatctacctt atgatcaggt tgagcgggct attaaagaaa aaaaactgat
    23401 acgtgttttg gataaattca caccagattt acccggttat cacctgtact atccacaccg
    23461 tcgacatgct ggctcggcat tctcattatt tatagatagg ctgaagtata aaggtgctgt
    23521 ttagcactac ttgctgatac attaatttaa ttcttctctt aacgtattct cagttccttt
    23581 caacgttttg gttatttttt attcttcgca caatggcgac agatgctgat tatgataccg
    23641 aaaacgggtt tgaacgtgcg aagcccgaac gtagtgttcg gagttctatg tgctttaccg
    23701 cattttggag actattattt acactaaatc tgatttgata tattgatact taaaacattt
    23761 gatgcttcct ttgtcacttt tttgatggaa gttgtttgca tttctttaag gcgaaacaaa
    23821 taattacgca tcaattttaa tgtcggttag agggaaactt atgaagcact ctgtttcagt
    23881 cacgtgttgt gcgctgttgg tcagcagcat ttctctttcg tatgctgctg aagttccgag
    23941 cggcacagta ctggcagaga agcaggagct ggtgcgccac attaaagatg agccagcgtc
    24001 gctggatccc gctaaagccg tgggcctgcc ggagattcag gtcattcgcg atctgtttga
    24061 aggactggtg aatcagaacg agaaagggga gattgtcccc ggcgttgcga ctcagtggaa
    24121 aagtaatgac aaccgtatct ggacttttac cctgcgcgat aacgcaaaat gggcggatgg
    24181 cacaccggta acggcgcaag attttgtcta cagctggcaa cgtctggtgg acccgaaaac
    24241 gctttcgcca tttgcatggt ttgccgcgct ggcgggaatc aacaacgcac aggcgattat
    24301 tgatggtaaa gccacgcctg accagcttgg cgtcaccgca gttgatgccc atactttgaa
    24361 aatccagctt gataaaccat tgccgtggtt tgtgaattta accgctaact ttgccttctt
    24421 cccggtgcaa aaagccaacg tagaaagcag taaagagtgg acgaaacccg gaaatctgat
    24481 cggcaatggc gcttatgttc ttaaagagcg cgtagtcaat gaaaaactgg tcgtagtgcc
    24541 gaatacccat tattgggata acgccaaaac ggtactgcaa aaagtgacct tcctgccaat
    24601 taatcaggaa tccgcagcca ctaagcgtta tctcgcgggg gatattgata tcaccgaatc
    24661 cttcccgaaa aatatgtatc agaagctgtt gaaggatatt ccggggcagg tttatacgcc
    24721 gccgcagctc gggacctatt attatgcgtt taacacgcaa aaagggccga cggcagatca
    24781 gcgcgttcgc ctggcattaa gtatgacgat agatcgccgc ctgatgaccg aaaaagtgtt
    24841 ggggacgggc gaaaagccag cgtggcattt tacaccagat gttaccgcgg gatttacgcc
    24901 ggaaccttcg ccgtttgaac aaatgagtca ggaagaactg aatgcgcagg caaaaacttt
    24961 gttgagcgct gctggttatg gtccgcaaaa accgttgaag ctgacgcttt tgtataacac
    25021 ttcagaaaac catcaaaaaa ttgcgattgc tgtagcatcg atgtggaaaa agaacctggg
    25081 cgtagatgtt aaattgcaaa accaggaatg gaaaacctat atcgatagcc gtaataccgg
    25141 caattttgat gttatccgcg cctcgtgggt aggggattat aatgaaccct ccactttcct
    25201 gacattatta acgtcaacgc attccgggaa tatttcacgc tttaacaatc cggcatatga
    25261 caaagttctg gcccaggcat cgacggaaaa taccgttaaa gcgcgtaatg ccgattacaa
    25321 cgcggcagaa aaaatcctca tggagcaagc accgattgca ccaatttatc aatataccaa
    25381 tggacgatta atcaagccgt ggctgaaagg ttatcccatt aataaccctg aagatgtggc
    25441 gtacagtcgg actatgtata ttgtgaagca ttaatgtgat gggaactggc gttacccttg
    25501 tggataacgc cagtgatgtc tgatttagcg accctggtcc ggcggtgtaa tgttatccat
    25561 atacagcgtc tggctgggga aggcaaagtc cgcgccgtgt gactgtacaa tatcgataat
    25621 cttcaaataa acgtcttgct gtgcagcaag ccactcagcc cataccgtgg ttttggtaaa
    25681 gcaataaacc ataatattta atgacgaatc agcaaactgg ttgaaataaa ccagtaaggt
    25741 ttgtcgctgg tcgatggccg ggtgattttt cagcatctca cgtacggctt cgacaataac
    25801 gcccactttt gccgcatcct cataacgtaa accaatggtc gtggtaatgc gccggttggt
    25861 cattcgtcca ggattttcta cgctgatcga cgaaaacagc gagttcggta cgtacaatgg
    25921 acgattatca aaggtcttaa ttttggtaat tcgccagcca atttccgtta ctgtaccttc
    25981 gatatttctg tccggtgaac ggatccagtc gccaatactg aaaggacggt cgaaatagag
    26041 cataatcccg gaaaagaagt tactcagaat atctttaccg gccataccga cagccagacc
    26101 accaatacca ccaaaggtca acaagccaga aaggctcatg ccgaaatgtt cgccataaag
    26161 cagaacaagc accacaataa tggtgatttt gataatgcgc gacatgatcc gcgcactggt
    26221 gatatcgcga ccttttttaa tctgctgttt ttcaaactga ttaatcagca gaaatagctt
    26281 aatcgtcaga ataaccgcaa tcagggacgt acagataaaa tcgataacgc ctggggtgat
    26341 aaatttgagt ttatagtttt ctataacata gttaatgatg ctaccaacag cactaataat
    26401 tatggtgtag attaaaaatt gcaccgcatg gaataaaaat ccttttcttt tacgatttcc
    26461 acggcgaaac caaaagctca tcagaatcaa tgctgcgcag ctaccgaaaa taatgaccag
    26521 attaagcgca ttatttgtaa acagttcagc gatcattgtt ttatcaggct cctccagata
    26581 attgtcgtca tgccggaaac ccctggcggg actattttac cgcgacaatt cattcagatc
    26641 atcaatagtc agggaaggaa gtagcaacat tagctaacta atcaatgcgt tgattgtaaa
    26701 tccagctaag aggtgagatt ttcagagcag acaacggtga aatgtcatgg tattgttacg
    26761 tttaggtaac aagaaatttg tctgcacaag gattacatca tgattatggc gaaactgaag
    26821 tcagcgaaag ggaagaaatt tctctttggt ttgttggcgg ttttcattat tgcggcgtcg
    26881 gttgtgactc gcgcgaccat cggcggcgtt atagaacagt acaatattcc gctgtctgag
    26941 tggacgacat caatgtatgt gattcagtca tcgatgattt ttgtttatag cctggtcttt
    27001 acagtgttgc tggcaatccc gttgggaatt tatttccttg gcggcgaaga gcagtaagta
    27061 aaaaataggc ccgataactc gggccttgtc agttattgaa gagtcgttaa tcgtcttctt
    27121 cgtcatccag ttcaacgggt gtctgatact ggtcaggttt aatgaccagc aggtcgcagc
    27181 gaagatgatc aatcacctgt tccgccgtgt tgccaaggaa tgctgctgaa ataccggtgc
    27241 gtccgaccgt gcccagaacc acaatccccg cctgtaagtg ctccgccaaa tcaggaatca
    27301 cctcttcggg cagacctttt tctacgtgcg tcatgttttc attaatgccg aatttctgcc
    27361 gcagagcttt cattgccagc aaatgttggc cacgaatggc atcgttataa acgctcgggt
    27421 caaattccgg cagttcaatc gcgatattaa ttggcgttac cggataagcg ccaaccagat
    27481 gaacttcggt atggttgact tgttctgcca gttcgatcgt ctctttgacc agtttttcat
    27541 tgagcgcatt atggtacggc tcttcactgg cgagattcac cgccaccagc gccttacccc
    27601 cttccggcca cggctggtct ttcaccatcc acacggggct tgggcatttg cgtaacagat
    27661 gccagtccgt tggcgtaaaa atcaccgctt ccagacggtc atgttggtgc gccattttta
    27721 gcaccaaatc gtgcccgccg ctgatcactt cctgaatgat ggcttcgaaa ggacggttat
    27781 gccagaccac tttaatttca atgggaacgc cagcattgag ataatatttt gcctgctcgt
    27841 ggatccaggc tgtacgctgg ctgatgacgc cctgacgcat agcggtacgt tcgtccggag
    27901 agagcagggt ggtcatttcg tatgagaagt catagatcgg caaaaaggct ttaattttgc
    27961 caccaatccg ttgatgtaaa taaacagctc gccgcaatgc tggttggtcg tcctggttag
    28021 gatcgataac aacgagcatg ttctgataca tagccataca gggtctcctt acaacaactg
    28081 tcaacgcagt ttgtaattaa aagattaacc catatctggt gaatgaaaca gtgatgaacc
    28141 ttctgtcaga tcaataaatc agaaaaattt aatgatatga cagaaggata gtgagttatg
    28201 cggaagaatc aggcaacgtt acgcgtatga ccagcaagct gggccagcgc atcgttattt
    28261 tcgatagtga tgtatttacc tttgactgcc agcataccgc ttttctggaa gcgacccagc
    28321 agacggctga tggtttcaac cgtcaggccc agatagttac cgatatcacc acgagtcatc
    28381 gtcaggcgga attcacgagg ggagaagccg cgttgggcaa aacgacggga caggttgtag
    28441 atgaatgcag ccagacgttc ctcggcattt ttcttcgaca acagcaggat catgtcctga
    28501 tcgcctttga tttcaccgct catcagacgc atcatctgct gacgcagatt cggcatttta
    28561 ccagacaaat cgtccagcgt ttcgaacggg atttcacata ccatcgaggt ttccagcgcc
    28621 tgcgcgaaac tcgggtgatg accgctgccg atggcatcaa atcccaccag atcgcctgct
    28681 aaatggaaac cagtgatttg ctcgtcgcct tgctcagtga tggtataact tttaatcgta
    28741 ccggagcgga tggcataaag cgatttaagt tcatctccag ccttaaacag cgtctggcct
    28801 ttctgaatag gcttcttccg ctcaatgata ttatcaagct gatcaagctc atgttcgttg
    28861 agtgtgaacg ggatgcaaag ctggctgatg ctgcaatcct ggcaatggat agcacaaccg
    28921 ccagactgaa tgcgccgtat aattcgcttt tccgggatca taggtctgct caagccgtaa
    28981 ttgatatttg tcaattttaa catcttttta gggagcaagt aagtctaagc aaaccttaac
    29041 agcagagaat tccgatatta gatgtaaata tatgtctatc tatttgaaaa cccttaagtt
    29101 gttaagggtg actttacata aaagtgtgaa caagctggca caaattgttt aatgtttaca
    29161 gcaaaagata accttcgtgg cgcaataacc actcttttcg ctgaactccg cctgcatatc
    29221 cggtcatggt gccgtttcgg ccaataaccc gatggcaagg tacgacgatg ctgatgggat
    29281 tcgatccgtt tgccgcacca acggcacgcg ccgcgccagg gcggcctaat tgttcagcca
    29341 gttggccata atgcattacc tgcccgcagg ggatagtgcg cagtgttttc cagacttcgc
    29401 gctgaaatgg cgtcaccccc gtggcagtgg gaagcgtatc aataatgcta agattaccgg
    29461 cgaaatattc acgaagcttg tcgcttaaac cacctgggtt ggtggcagaa atgcgctcat
    29521 agccatcttt gcgataatgg atgtccagca gctgcaccat gcgttcgctg tactcttccc
    29581 attcaaccgc ccgcaggcga aattgctcat cgcaaatcac ccacagtgga cccagtggcg
    29641 tggcaatttt ttcttcaagt aatctcagca tccgttctct cttaagacaa acgtgggtaa
    29701 atacccggac ctatcggcaa gccaacaaga taccacgtca gcaacatcag cagccatacc
    29761 accgaaaaga ttaatactaa caaaaagtaa gtaccaggtt tagcgtctgg tttgtcgaag
    29821 tatttaagca taaagcgatt ttttgatttt tctgcctgaa gaaatgaggg caagccatca
    29881 aattccagtc agcgaaattt taccgtaggc tatccgttat ttgattctgt ggcgtgatag
    29941 ccgccagaca ccactgatct cgtcactcat tccccaatcc cggcgagaat gtggttattt
    30001 acagagctaa aaaatgaata aaaaaataga gcctgtcgac atccgccaga ctctacagta
    30061 cacacagcag tgcatcctcg tcttaaatcc ggcctggggt cctggtgacc ggtgacagtg
    30121 ttataacggt tttacccatc cgttgccaat tttcttcagt gacagatttc acgaaaatat
    30181 aagttaaatc atttgtcttg tgattgcata atcatgcatc gttaatcgac gagtaaattt
    30241 accgggtgag tagcccgttc gacgcttaaa catattgccg aacgattcag ctgaggcata
    30301 gcccagacga tgggcaattt tggtcaacga atatccatca tcaagaagcg tcactgcaga
    30361 aataacgtgc atttgctgaa cccagtggcg aaatgttaat cccgtctctt taacaaataa
    30421 gcgcatgagt gtacggctac tgacgttaat tttatccgct agattatcca gtgttggcag
    30481 atacccttgg ttgtttagca aattctcaca aagctgctgt agccgctcat catttggcca
    30541 agggagttca agtgacattt tcacccccat ttttatttcc tgaagtatca gtgtctcgag
    30601 ggctgaaaaa cgtaactgtt gtttatctgt ggtactttgc gacggtggaa gttggttaat
    30661 tgcaattagt aattccctta acagggggga aatacccgtc atgaagcaaa cttttcccag
    30721 cgtttcactt tccgcttctg aaaccagtac ggtttttaaa agtatgttgc ctgtcgcaac
    30781 aaacttatgc ggcatttgtg ggggcagcca aagcgcgctg ctggcatgca ctagccagac
    30841 gctttctcca gcccatatac gaacgactcc ttcagccggg taaagcaact gggcatagag
    30901 atgtttatgc cagggaactg tgcctccatt cagatgttcc gaatccagtc cgataatgtg
    30961 atgccctggt gatgctttgg gttgcagtgt gattgtcatt tacagtaggt attttgtcag
    31021 aaatcatcaa ataagccatg ttaaatttta aatatgacgt atgcaagttt ccggatatat
    31081 acggtgtaaa gagtgatgat aatttctaaa aaacagctta ttggggtggt agcgatcggt
    31141 attcttttgg ctggcgttgt cttttttatc tggtgggtaa gcaaaggaag atttatacag
    31201 acgacggatg atgcttacat tggtggtaat atcaccacgg tggcaagtaa agtttctggt
    31261 tatatttctg ctatagaggt gcgcgataat cagagtgtaa aaaaaggcga tattatcctg
    31321 cgtctcgatg atcgggatta tcgtgcaaat gtcgccaggc ttgaggcaaa aataaaaagt
    31381 agcaaagcca atctcgaaag cattcaagcc actattgcga tgcagcagtc gataattcaa
    31441 agtgccagtg aaacatggca ggcagtaaaa catgaggagc aaaagcgcct gcgagatact
    31501 gaacgttatg aaaaactcgc acaatcagcg gcaatatcgc aacaaattat tgataacgcc
    31561 aggtttgact accagcaggt cgcagcaaag gagcgcaaag ccgcgaacga tttcctggtg
    31621 gagaagcaac ggctggcagt tttatctgct caggaagaaa acgtacgtgc aagtatcgaa
    31681 gaagttcagg ccgctttgac tcaggcacta ctggatctgg aatataccct tgttcgtgcg
    31741 ccgattgatg gcattgtcgc aaaccgcagc gcccatacag gcagttgggt ggaaggcggc
    31801 acatcgttag tttcactagt accagtcagt gagttgtggg ttgatgcaaa ttataaagaa
    31861 aaccaaatag cgggaatgaa gccaggaatg aaggctgaga tccgtgctga tattctcaaa
    31921 ggggaagtgt ttcacggtca cattgaaagc ctttccccag cgacaggagc cagtttcagc
    31981 ttgattccga tagaaaatgc cactggcaat tttaccaaaa ttgttcagcg tgtcccggta
    32041 cggattgtat ttgacgacgc aaaggaatta aagcaacttc ttcgcccggg actgtccgtc
    32101 accgtcagtg ttgatgagag atgatgtcta tgcgtaaaca tattgctttc gccagtatgt
    32161 gtattggatt atttattgct cagcttgata ttcaaattgt atcgtcatcc ctaaatgaaa
    32221 taggtggtgg tctgtccgca ggtaaagacg aaatggccta gcttcagacc agctatttaa
    32281 ttgccgagat tattgttatt ccgctctctg ggtggctgtc acgggtattt tccactcgtt
    32341 ggctttttac tctatccgcg ggaatattta ctttaatgag tatcgcttgt ggacttgcat
    32401 ggaatattca gatcatgatt ttttttcgtg ctttacaggg agttgctggc gcatccatga
    32461 tccctttagt tttcacgacg gcgtttattt attaccaggg taaagagttg gggttggctg
    32521 ctgcggtagt cagtgcgctg gcctcactgt caccgacgct agggccaact ctggggggct
    32581 ggatcactga taacctcgac tggcgatggc tattttatat caatatatta ccgggcatat
    32641 atttagtttt gagtattcct tttctggtta attttgataa acctgatctc tctttactga
    32701 aagttgctga ttatccgagt attatcctgt tggccatgac actgggatgt ctggaatata
    32761 cccttgaaga aggagcgcgc tggggatggc tggatgataa tactattttg ctgacatcag
    32821 ttctggcatt ggtaagtttt attttatttg ctgcgcgtac acttacaata tccaatccga
    32881 taatggatct tcatgc
//
LOCUS       DAZFFE010000045        32564 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFFE010000045 DAZFFE010000000
VERSION     DAZFFE010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.045"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(143..421)
                     /locus_tag="ACHJ69_002298"
     CDS             complement(143..421)
                     /locus_tag="ACHJ69_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015274.1"
                     /translation="MSTFNTRKDRDRNGKAKPHKHSGIPALTGKWRHPSMDCWVNYST
                     PGDHEWKRLYTTLRRRADDRRQCHRVMQGDDPEGIVWFPDCYPQIYYY"
     gene            complement(780..1070)
                     /gene="nadS"
                     /locus_tag="ACHJ69_002299"
     CDS             complement(780..1070)
                     /gene="nadS"
                     /locus_tag="ACHJ69_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO5015275.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(1071..1382)
                     /locus_tag="ACHJ69_002300"
     CDS             complement(1071..1382)
                     /locus_tag="ACHJ69_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5015276.1"
                     /translation="MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            1611..2519
                     /locus_tag="ACHJ69_002301"
     CDS             1611..2519
                     /locus_tag="ACHJ69_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5015277.1"
                     /translation="MALEKGIAELVEEFIAAGRPSSRQQSITQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRIIAVQYRLAPEHTFPAAHDDAQRGAELVRQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPIWRNDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(2583..3524)
                     /gene="fabY"
                     /locus_tag="ACHJ69_002302"
                     /gene_synonym="yiiD"
     CDS             complement(2583..3524)
                     /gene="fabY"
                     /locus_tag="ACHJ69_002302"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO5015278.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3569..4006)
                     /gene="dtd"
                     /locus_tag="ACHJ69_002303"
     CDS             complement(3569..4006)
                     /gene="dtd"
                     /locus_tag="ACHJ69_002303"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO5015279.1"
                     /translation="MIALIQRVTRASVTVEGEVTGKIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(4003..4875)
                     /gene="yihY"
                     /locus_tag="ACHJ69_002304"
     CDS             complement(4003..4875)
                     /gene="yihY"
                     /locus_tag="ACHJ69_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO5015280.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4869..5468)
                     /gene="yihX"
                     /locus_tag="ACHJ69_002305"
     CDS             complement(4869..5468)
                     /gene="yihX"
                     /locus_tag="ACHJ69_002305"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO5015281.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPDD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5567..6352)
                     /gene="csqR"
                     /locus_tag="ACHJ69_002306"
     CDS             complement(5567..6352)
                     /gene="csqR"
                     /locus_tag="ACHJ69_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5015282.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(6386..7282)
                     /gene="yihV"
                     /locus_tag="ACHJ69_002307"
     CDS             complement(6386..7282)
                     /gene="yihV"
                     /locus_tag="ACHJ69_002307"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFO5015283.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVAKNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVAFSDHAAFSEPGLARLTGVKEMASALKQAQMLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATGGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            7450..8346
                     /gene="yihU"
                     /locus_tag="ACHJ69_002308"
     CDS             7450..8346
                     /gene="yihU"
                     /locus_tag="ACHJ69_002308"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO5015284.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLCVFDVNTEAVQHLVDKG
                     ATPAANPAQAAKGAEFIITMLPNGDLVRHVLFGENGVCESLSSDALVIDMSTIHPLQT
                     DKLIADMQARGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            8370..9248
                     /gene="yihT"
                     /locus_tag="ACHJ69_002309"
     CDS             8370..9248
                     /gene="yihT"
                     /locus_tag="ACHJ69_002309"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFO5015285.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKTLSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNEMCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            9264..10505
                     /gene="yihS"
                     /locus_tag="ACHJ69_002310"
     CDS             9264..10505
                     /gene="yihS"
                     /locus_tag="ACHJ69_002310"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFO5015286.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKKSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            10572..11474
                     /gene="yihR"
                     /locus_tag="ACHJ69_002311"
     CDS             10572..11474
                     /gene="yihR"
                     /locus_tag="ACHJ69_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFO5015287.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAIHGLLAWRGWQI
                     SELSATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAIDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKTHRLFFNIHGQHN"
     gene            11535..12410
                     /locus_tag="ACHJ69_002312"
     CDS             11535..12410
                     /locus_tag="ACHJ69_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO5015288.1"
                     /translation="MDFFTLFSPDGVMLETNVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFILRLPIYDRSWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLA
                     FGKKVAAIRRAEESK"
     gene            12516..14552
                     /gene="yihQ"
                     /locus_tag="ACHJ69_002313"
     CDS             12516..14552
                     /gene="yihQ"
                     /locus_tag="ACHJ69_002313"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFO5015289.1"
                     /translation="MDTPRPQLIDFQFHQNNDSFTLRFQDRLILIHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDHGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAKRGYLAKDAAGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            14598..15983
                     /gene="yihP"
                     /locus_tag="ACHJ69_002314"
     CDS             14598..15983
                     /gene="yihP"
                     /locus_tag="ACHJ69_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5015290.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            16026..17429
                     /gene="yihO"
                     /locus_tag="ACHJ69_002315"
     CDS             16026..17429
                     /gene="yihO"
                     /locus_tag="ACHJ69_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5015291.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAIPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTTP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYCWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYIPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEIETEEKTASVTL"
     gene            17497..18189
                     /gene="ompL"
                     /locus_tag="ACHJ69_002316"
     CDS             17497..18189
                     /gene="ompL"
                     /locus_tag="ACHJ69_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFO5015292.1"
                     /translation="MKNINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRMG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRYNHNNYSSTDLNGELDNNDTYEVGTYWNFKITEKF
                     SYTFEPHYFMRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(18291..20114)
                     /gene="typA"
                     /locus_tag="ACHJ69_002317"
     CDS             complement(18291..20114)
                     /gene="typA"
                     /locus_tag="ACHJ69_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO5015293.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            20487..21896
                     /gene="glnA"
                     /locus_tag="ACHJ69_002318"
     CDS             20487..21896
                     /gene="glnA"
                     /locus_tag="ACHJ69_002318"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO5015294.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            22070..23119
                     /gene="glnL"
                     /locus_tag="ACHJ69_002319"
     CDS             22070..23119
                     /gene="glnL"
                     /locus_tag="ACHJ69_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO5015295.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRITESIHKVAERVVTLVSMELPNNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            23131..24540
                     /gene="glnG"
                     /locus_tag="ACHJ69_002320"
     CDS             23131..24540
                     /gene="glnG"
                     /locus_tag="ACHJ69_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO5015296.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESNVPE
                     STSHMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLATALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            24652..24762
                     /gene="yshB"
                     /locus_tag="ACHJ69_002321"
     CDS             24652..24762
                     /gene="yshB"
                     /locus_tag="ACHJ69_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO5015297.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(24995..26368)
                     /gene="hemN"
                     /locus_tag="ACHJ69_002322"
     CDS             complement(24995..26368)
                     /gene="hemN"
                     /locus_tag="ACHJ69_002322"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO5015298.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLLFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(26557..27066)
                     /gene="yihI"
                     /locus_tag="ACHJ69_002323"
     CDS             complement(26557..27066)
                     /gene="yihI"
                     /locus_tag="ACHJ69_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO5015299.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(27185..27351)
                     /locus_tag="ACHJ69_002324"
                     /pseudo
     CDS             complement(27185..27351)
                     /locus_tag="ACHJ69_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27648..28280
                     /gene="yihA"
                     /locus_tag="ACHJ69_002325"
     CDS             27648..28280
                     /gene="yihA"
                     /locus_tag="ACHJ69_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO5015300.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(28662..31448)
                     /gene="polA"
                     /locus_tag="ACHJ69_002326"
     CDS             complement(28662..31448)
                     /gene="polA"
                     /locus_tag="ACHJ69_002326"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO5015301.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQPPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAARPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKEWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            31812..>32564
                     /locus_tag="ACHJ69_002327"
     CDS             31812..>32564
                     /locus_tag="ACHJ69_002327"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO5015302.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLS"
BASE COUNT         7771 a         8383 c         8135 g         8275 t
ORIGIN      
        1 agaatatttt ttgattgggt cttcatttgc ttcatcagga tggtgtaccc gaaaaagtca
       61 gcagctttac cgcaccaggg tttaaggtga aatctaccat tcataacgac cggtcagtgc
      121 cgcaccaacc ggtctttaat tgttagtagt agtaaatttg tggataacag tccgggaacc
      181 agacgattcc ttccggatca tccccctgca ttacccgatg acattggcgt cgatcatctg
      241 cgcgacggcg aagcgtagta tagagccgct tccattcatg atcgcccggt gtactgtagt
      301 ttacccaaca atccattgaa gggtgacgcc atttacctgt aagtgctggg atccctgaat
      361 gcttatgcgg cttcgcttta ccattacgat ccctatcttt acgcgtatta aacgtggaca
      421 tttttagtct cctgttaaga gttctaaaaa tgtgcttcct taaaatagtt catcattttc
      481 ccttaattcg catagcgtgc ttttgcacga atttccgata cgcggggacc attatgatag
      541 cgtcgatgcc cacacatcca acaagaacaa atacagggag tacgaaagca aatacctgat
      601 gcttttttgc ttccatcgcc tgcagtgcga taacgacagc ggattttttt taatcgcacc
      661 atatgatggc gacgttcagc acgattacgg gtcatagcaa aactcctgag caggcggcac
      721 tatgccgctt tcataggagt tctgaatgct ggtgctttct gtatggcata accatgcctc
      781 taacggttaa gtgcttgcag cacatgttgc ggatcattgg caatcgctcg cagcaaagcc
      841 tttgcaggcc cggtcggtga acgtcgcccc tgctcccagt tacgcaacgt gtctacgctt
      901 acggaaataa gatcggcaaa tttagattgt gacaaccctg aagcgcggcg tatttctttg
      961 attttcatag catcaacttc aaaagtccgt gaaggctgtc gcgttccttc aataatttca
     1021 ttcatctggg ccatgctgtg agtcaaccgc tcaaaaagtg ccttatccat ttaccacctc
     1081 gtatttaaca accggagcat tgctttttcc tggggagaca gatcatcttt aataccctta
     1141 cgataaataa gcagtaatct gatctcataa tgtttaacct gatgaaagta aataaccctg
     1201 acaccagcac gtttcccctt tcctcctgag acccatcgaa cctttcttaa cccaccagtt
     1261 tcaggtatta cctcaccata gtcaggattc aaggccagga aaaactgaaa acgactgaat
     1321 tcatcgtcgt tcagcagttt ttgaacatct tcagtaaaaa tttccgtttc gatgaagagc
     1381 ataattatac gccattggct tagagtaaca atcctttttg tacgtcattg acgtaccatt
     1441 gaaaccacta gcttactgat agtgctccag caaaaccacg tctctgccgg gtttgtcttt
     1501 acatttgcga ggcgctttcc agatttttca cgcccgtgaa tttccctgta tccatccagc
     1561 aaaaacgggt gtataaaact gtacattcat acagcaaaat gagttctact atggcactgg
     1621 aaaaaggaat tgcagagtta gtcgaagagt ttatcgctgc gggtcgcccc tcatcgcgcc
     1681 aacaaagtat tacgcagcgc agagagggct atatcgccag cgctgtcctt gcgggtgaga
     1741 cagaaacgcg ggtagatatt cagactatcg aacttgaagg gatgacctta cgaatagtgt
     1801 caccgctgaa tgccccgaca ttactcccga cgataattta ttactacggt ggctgttttg
     1861 ttagcggcgg gttcgcaact cacgacaacc agctgcgcca gttagcctat tacgggcaat
     1921 gtcggattat tgccgtccag tatcggctgg caccggaaca tactttcccc gccgcccatg
     1981 atgatgcaca acgaggtgca gaattagtcc ggcaacacgc cgaacgactc ggtgttgata
     2041 aacaacgaat aactcttgcc ggtgacagtg cgggagggca tctggcgctg gtaactgctt
     2101 tgcgattgaa gcgtgctggc gagtggcaac cagcacaact catcttaatt tatcctatgc
     2161 ttgatgcgac tgcgcatttc gaaagctaca tacgcaatgg tcacgattac atcatcacac
     2221 gcgacacttt actgagcggc tttgagatgt acctgccagg cattgaacgc aggcatcctg
     2281 aagccagccc tatttggcgc aatgatttca atggcttgcc acctgtacat atcattaccg
     2341 ctgaatacga cccactttgc gatgaaggtg aagcgttgta tcaccgcatg acagggcaag
     2401 gagtgcaatg cacctgccaa cgctaccttg gcgttatcca cggatttttt cagttaggtg
     2461 gtatcagtga ggctgcgcga tcggcgctac gggatgttgc ctggagagcg ggaaggtaac
     2521 tccggaccag ggcccagaaa aggttttcag attgaaaacc ttttcagtca agcaaaacgc
     2581 ccctactctt cttcgttccc gccctcttca tacgggccaa atggcttcgc gggcagaacg
     2641 atatacgtgc cttcaaacac tgcacccggc gtctcgtcgc caaagatttc tacttgcatc
     2701 tgcacccgtg cttttcgtcc gcgcgccaga cggtcgagat cgccacttaa ggcaccaagg
     2761 tcggctaccg catggggttt tccgctaatc ggcttgctgt agcggatatg cgcatccgcc
     2821 agaataatcg ttccgccgag gtggcgttcg cgcagcatca gccagataag cccccaaccg
     2881 gtgagcgttg ccagcgagaa taaactcccg gcaaacagcg tgtgatgcgg attctgattg
     2941 cctgtttccg gcatggtggt gataaatttt tgcccggtat attgctgaat gcgcacaccc
     3001 attttttcac tcagcgggat gtgttcatac cacgcttgct ggagctgggc gcaccagtcg
     3061 ccgcgatgaa gaatatcatc cagagtggca acaggtttta tcatcagaaa atggcgaatc
     3121 ggcgtggtgg ttggcgtggt gatttctccc tgattaacaa accccagctt ggcgaaaaac
     3181 tccaccgcgt cttcacgggc gctacaggtc acgcgcttaa cgccttcctg acgtgccacc
     3241 gactccaggg tcatcgccat cagcgtgcct aaccctttgt cctgtacgtc gggatgaaca
     3301 gccataaagc gaatggacgc ttcattgtcg gcattaatat acagtcggcc taccgctacc
     3361 agattaccct gctcgtcgac gaccatctgg tgatgcgcca tcgcatccca cgcgtcgcgt
     3421 tccgaacctt ttggttgatg caggggctta cgcaacattt cccagcgaaa ctgatagtaa
     3481 cgctctaatt cttcttctgt ttgtggaacc cgaaggtgat acatagcgat actctctctt
     3541 gtttcccgtg acaaccctgg aagctggctc atacctgcaa ccagaatgtc acggggccat
     3601 cattgaccag cgatacctgc atatccgcag cgaagcgtcc tgtttgcgtg ttcatctctt
     3661 gctgacggca gcgttcgacg aaatagtcat ataacgcctc tgcgcgatcc ggtgatgcgc
     3721 ctttggagaa acttgggcgc atcccccgtt cggtatctgc ggcaagggta aactgggaaa
     3781 ccaccagtac actgccgccc gcctgttgca cgttgagatt catcttgcct tcggcatcgc
     3841 taaagatgcg gtagccgagc acacgctcgc acagacggtt tgctttctgt tcgtcgtcat
     3901 ccttttcgac acccaataac accaaaagtc ccgcgccaat tttacccgtc acttctccct
     3961 ccacggtgac gctggcacgg gttacgcgtt gaattaatgc aatcatggtt cgtcgtcttc
     4021 ttcttgttca gcagcttgtt ttaatttgcg gtattccccg agagtgacag taatttccgc
     4081 gccaagcaag acgatacacc atgtccagta gacccaaaca aagagaatgg ggatcaccgc
     4141 cagcacaccg taaatgagct gatatgatgg gaacatggtg atataaagcg cgaaaccttt
     4201 ctttcctgct tcgaacagga gcgcggcgac aaacgcgccg acaatcgcgt cgcggttagg
     4261 tacacggatg gtaggaacga tgctgtacag cagccagaaa gagatccacg acaacagcag
     4321 cggaaaaata cgcaacacat tatcgatgac agtattgaga tcgctcgccc agcgcagaga
     4381 gagcagatag gagctgatcg ccagactggc ccctgccaac agcggcccca gcgttaaaat
     4441 catccagtac acggcaaacg agtaaatttt gggtcgcgct cgtttactgc gccagatggt
     4501 attcaacgcg ctatcgatgg agtacatcaa caataacgcc gtgacgatca gcccgcacgc
     4561 cccgacggcg gtcattttgt tggaattggc gacaaactgt tcgatatatc gctggataac
     4621 atcgccagta gcaggcagaa agttggcaaa aataaagtga cgcaactgaa tgctgacgtc
     4681 ggaaaacatg ggaaaagcgg cgaaaagcgc aaaaacaacg gcaaccagcg gcactaatga
     4741 gagcaacgac acataggcaa ggttacctgc caaggttgtc atgttgtcct catcaatgcg
     4801 ttgccagagt agttttagcc aggcccatag tggacgggta cgatgcctgg ctttgtcctg
     4861 aatggttttt agcataacac cttcgcgaaa tagtccggga tggtggtttt atctttcacc
     4921 agaatactag taatgcctag ctgattagct ccttctatat tatcggcgtt atcgtcgaaa
     4981 aagaccgtat cgtctggtga aaaaccttct gcctgcaaaa catgctgata aattcgtgct
     5041 tcaggtttgc gcatacccaa gtcttgcgac agatagatat ggtcagcagc atcacggatt
     5101 tccgggtatt cctccggcca gaaggtagta tgcaggcggt tagtattgga aagcaccacc
     5161 acgcgatgcc cctgctcacg cagtttatgc atgatggcga tcacttccgg gcgcagcgcg
     5221 acaaacaccg cctgccagcc gtgagagaac tgctcgtagc ttagcggtag agccatctca
     5281 tgacacagcg cctctgcgaa cgcttcgtcg ctaatttccc cacgctcatg ctgatgaaac
     5341 gcctcgccca tgtggaaact cttcttaagc gtagccagtg gaacacgcgt taaatcgctc
     5401 caggctccca gcacacggtt aaagtcgata tcgacaatca cattacctaa atcaaagata
     5461 tagagcattt ttgcctcctt tcgcgccaca agaaattaac tgtagcggga aaggatgggt
     5521 ttggctatgt agtcaggtat gaagccaggt caacggtcac aaaattctac gcgtcttcct
     5581 ggggaaggat gatttcaatt tggctgtctt gtaagcgtga ttttagcgca gcgggcggca
     5641 gctcgtcggt aaagagcgca gtgacctgtg ccacattacc aatttcaacc gccgccgaag
     5701 catgatactt agtgtgatcg gcgaccagca gaatatttct cgcgtgcgcc atcatcgttt
     5761 tcaccacgtt agcttcgtta acatcaaact ccatcaacgc gccatcgctc tcaattgcgc
     5821 ctacacttgt taccagataa tcggcgcgaa aatcggccac aaaggacgcc gctgaagggc
     5881 caatgatccc actgttatgg gaacgcagcg ttccgccagg caccatcact tcaaagcgcg
     5941 ggttgtggta aagaatatgc gccacacgca ggctgttggt gattatccgc aaatggttat
     6001 ggttaagtaa cgcccgggca acatgctcaa cggtcgtacc aatggtgata aatattgttg
     6061 aaccatcagg aatatagtct gccaccgctt cggcaatcgc ttttttttct tcggtttgcg
     6121 aaacctcacg ctgctcgaac gccgtattaa cgacgctgga agcccgaccc gcgccaccgt
     6181 gatggcgcgt aatcagtcct tgctcgctta atttacgaat atcccggcgg accgtctgcg
     6241 tggagacatc cagcagattt gccagctcat caatattcat atacccacgc tcggcgatca
     6301 gcatgaggag ttggtcgtgc cgcgggttac cggttagttc ggtaaggctc atgaaaattc
     6361 ctcgaaaaac catcaccctg gcattttata caaaaagtga caaaaaagat cgggtttgat
     6421 cacagtcagg gatcccggct cgtccaccgg gacgcgtgca tttcaacgcc gctactccgc
     6481 tggcgaagcg gactgattcc gctaaatccc cacctgttgc cagcgccacc gccaatgcgc
     6541 cgtgaaaaac atcacccgca cctgtggtat ctaccacatc aactttgaag gccggttgat
     6601 gctgtcgccc gccattctcc agccagtcac agcctgcact gccctgggtc acatagacat
     6661 gaccatttgt gagcatttgt gcctgtttta gggcactcgc catctctttc acgcccgtta
     6721 agcgcgccag acccggttct gaaaaagctg catgatcgct gaacgccacc agttcgctga
     6781 tatcctgcgg cgtaatatcg ccgtccagca cggtcatcac gcccgcctgc cgcgccagag
     6841 tgaaggcttt tttcgcgccg tcgtgccagc gtacatctgc cagcaccacg tcccactgag
     6901 agaaatcaat ttcctccaac cactctgcat caggcagcag gtctgggctg ggatagttaa
     6961 tgatgatgcg ctcacctttg gcatccacca tgatggcgga ttgcgaagat ttcgcctggt
     7021 tataccgttt ggtgtaacgg gtgttaacac cccaggattc cagctctgcc agcaggctgt
     7081 tgcccgtgtc gtcgtcaccg acgcgaccaa taaaatcgac ctgcgcacct agtctggccg
     7141 ccgcaactgc tgctgtagcc gctggcccgc cgccaacttc cgtataattt ttcgccacgt
     7201 atttaccgct ctccgtcggc aatccttcca cgtaatagat gcgatccatc acggtaattc
     7261 ctacacaagc aacacgaatc atggtcattc cttaagcatt ttgggatgcg ttcattttaa
     7321 tttcatcaat tgtttaaaaa gtgacggctg tcaatttttt gacgataaat tacaaatacg
     7381 atcaaaaaca gacaagcaag gcatgtcata aatgacaaaa agtgacatca ctgtattcag
     7441 gagagagtta tggcagcaat cgcgtttatc ggtttagggc aaatgggttc gccaatggcg
     7501 agcaatttat tgcagcaagg gcatcaactt tgcgtcttcg atgtgaatac cgaagccgta
     7561 cagcatctgg tcgacaaagg ggcgactccc gctgctaacc cagcgcaggc cgctaaaggt
     7621 gccgaattta tcattaccat gttgccgaat ggcgatctgg tgcgtcacgt gttgttcggt
     7681 gaaaacggtg tctgtgagag cttatcttcc gatgcgctgg tcattgatat gtcgaccatc
     7741 cacccgctgc aaaccgataa attgattgct gatatgcagg ccagaggctt cagcatgatg
     7801 gatgttccgg taggccgtac ttccgcaaat gccattaccg gtactctgct actgctggct
     7861 ggcggcactg ctgaacaagt tgaacgcgcc acgccgatcc tgatggcgat gggcagcgag
     7921 ttgatcaacg cgggcggtcc gggcatgggg atccgcgtta agcttatcaa caactacatg
     7981 agcatcgcgc tcaatgcgtt gtcggcagaa gccgccgttt tgtgcgaagc cctcaatctt
     8041 cccttcgatg ttgccgtcaa ggtaatgagc ggtactgccg ccggtaaagg ccacttcacc
     8101 acttcctggc cgaacaaagt cctcagcgga gatctttctc ccgccttcat gatcgatctt
     8161 gcccataagg atcttggcat cgcccttgat gtcgccaacc agttgcatgt gccgatgcca
     8221 ctgggggccg cctcacggga ggtttatagc caggcgcgcg cagcgggtcg cggtcgccag
     8281 gactggtccg ccattctgga gcaggtccgt gtcagtgccg ggatgactgc caaagtaaaa
     8341 atgtaacgac tggataaagg aataaatgaa tgaataagta caccatcaac gacattacgc
     8401 gtgcatcggg cggttttgcc atgctggcgg tcgatcagcg cgaagccatg cgcatgatgt
     8461 ttgccgcggc tggcgcaccc gctccagtag ccgatagcgt tttaactgat ttcaaagtta
     8521 acgccgcgaa gactctttca ccttatgcat cggcgattct ggtagatcaa caattctgct
     8581 accgccaggt ggttgagcaa aacgcgattg ccaaaagttg cgccatgatt gtcgccgccg
     8641 atgagttcat tcctggcaac ggtattccgg tagatagcgt ggtcattgac cgcaaaatca
     8701 atccgctaca aatcaaacaa gacggtggca aagccttaaa actgctggtg ctgtggcgta
     8761 gtgatgaaga tgcgcagcaa cgcctggata tggtgaaaga gtttaacgaa atgtgccact
     8821 cacacggtct ggtaagcatc attgagccag tcgtccgccc accgcgtcgt ggcgataaat
     8881 tcgatcgcga acaagcaatc atcgatgcag ccaaagagct gggcgacagt ggcgctgatc
     8941 tctacaaagt tgaaatgccg ctttacggta aaggtccgca acaagagctt ctcagtgctt
     9001 cacaacgtct gaatgaccat atcaatatgc cgtgggtgat cctctcttcc ggtgtcgacg
     9061 aaaaactgtt cccgcgtgcc gtacgcgtgg caatgacagc gggtgcatcg ggcttcctgg
     9121 ctggtcgtgc cgtctgggca tcggtcgtcg gtttaccaga caacgagctg atgctgcgtg
     9181 acgtttgcgc accgaaatta caacaacttg gcgatatcgt cgacgaaatg atggctaaac
     9241 gccgttaaga tggggataca aaaatgaaat ggtttaacac tttaagtcac aaccgttggc
     9301 tggaacagga aaccgatcgc atctttgatt ttggtaaaaa atccgtagta ccgaccggat
     9361 ttggctggtt aggcaataaa gggcaaatca aagaagagat gggcacccat ctgtggatca
     9421 cagctcgtat gttgcacgtt tattccgttg ctgcggcgat gggccgacct ggcgcttact
     9481 cgttggttga tcacggtatc aaagccatga atggcgcact gcgcgataaa aaatatggcg
     9541 gttggtatgc ctgcgtgaat gacgagggcg tggtggatgc ctccaaacag ggctatcaac
     9601 atttctttgc tctgctgggt gctgccagcg ccgtcactac cggtcatccg gaagcgcgca
     9661 agctgctcga ttacaccatt gaaattatcg agaaatactt ctggagcgaa gaagagcaga
     9721 tgtgcctgga gtcctgggat gaagccttca gcaaaaccga agagtatcgc ggcggcaatg
     9781 ccaatatgca cgcggtggaa gccttcctga ttgtctatga cgtcacccat gacaaaaaat
     9841 ggctggatcg cgcgattcgc gtggcttccg tgattatcca cgacgtcgcc agaaataatc
     9901 attatcgcgt taacgaacac ttcgataccc agtggaatcc gctgccagat tacaacaaag
     9961 ataacccggc gcaccgcttc cgcgcgttcg gaggtactcc gggccactgg atcgaatggg
    10021 gccgtttaat gctgcacatt cacgcggccc tggaagcccg ttgcgaacaa ccgccagcat
    10081 ggctgctaga agatgccaaa ggtctgttta acgccaccgt gcgcgatgcc tgggcacccg
    10141 atggcgcgga cgggattgtt tataccgttg actgggaagg aaaaccggtg gtccgcgaac
    10201 gtgtgcgttg gcctatcgtc gaagcgatgg gtacggccta cgcgctctac accgtcactg
    10261 gcgatcgcca gtacgaaacc tggtatcaga catggtggga ctactgcatc aaatacctga
    10321 tggactacga aaatggttca tggtggcagg agctggatgc ggacaataag gtcaccacca
    10381 aagtctggga cggcaaacag gatatttatc atctgctgca ttgtctggtg atcccacgta
    10441 tcccgttagc gccaggcctg gctccggcgg ttgcggcggg tctgctggat attaacgcga
    10501 aataactgac taaaaaaacg acatatcagg ctgcacgctg ggtatgtcgt tattcaataa
    10561 aggtacctcg tatgcaaatc accaacatgc attgcagtgg acagaccgtt tcacttgccg
    10621 cgggggatta tcacgcgacc attgtgaccg tcggtgcagg tctggctgaa ttaacctttc
    10681 agggctgtca tttagttatt ccgcataaac ccgaagagat gccgctggca catctgggca
    10741 aagtccttat tccgtggcca aaccggatcg ccaacggctg ttatcgctat caggggcagg
    10801 aatatcagtt acccattaat gaacatggtt caaaagccgc tattcatggg ctgctcgcct
    10861 ggcgtggctg gcaaatcagc gaattaagcg ccaccagcgt cacattaacc gcctttctgc
    10921 cacccagtta cggctacccg tttatgctgg cttcgcaggt ggtttattcg ctcaacgccc
    10981 gcacgggtct ttccgttgag atcgcaagcc agaatatcgg cactgtcgca gcgccttacg
    11041 gtgtgggtat tcacccttat ttaacctgca acttaacatc tgttgatgaa tatctgttcc
    11101 agcttccggc aaaccaagtt tatgccatcg atgagcacgc taatccgaca acgcttcacc
    11161 acgttgatga gctggattta aatttcacgc aggcgaaaaa aatagccgcg acaaaaatcg
    11221 accacacctt taaaaccgct aatgatttgt gggaaatgac tattacccat ccacagcagg
    11281 cgctttcggt gagcctctgt tccgatcaac cctgggtgca ggtttatagc ggtgaaaaat
    11341 tacaacgtca gggactggca gttgaaccaa tgagttgccc gcctaacgcc tttaattcag
    11401 gtatcgactt attattactg gaaccaggga aaacgcaccg cctgtttttt aatattcacg
    11461 ggcagcacaa ttaattttat taagaaaaga acgagatcat tgtttagagt tgtatttaca
    11521 caacaggaaa tattatggac ttttttacgt tattttcacc ggatggggtc atgctggaaa
    11581 caaacgtggt atgtatcgtt atcgcgttaa tgtttcttta tacctttgtg gggatatgcg
    11641 cgggatttgg cggtggttta accaccatgc cgctcgtcac gttaatgctc cccgtaaaaa
    11701 tggcaactcc gctctcggtg attgtcggca ccgcgaccgc cttgtacgcc acctggctgt
    11761 cacgtaaaga aacggattgg cgatcggcaa tggtactgat tattttctcg tttctgggaa
    11821 tacccgtcgg tttatatgcg ctgtcgtatt tacccgacca ttttatgaaa gtgggcttag
    11881 gcggattcct tatcctgtac tccttttaca gtatgtttat tctacgcctg ccgatttatg
    11941 accgtagctg gatcgccgca cctatcggcg ccgtggcagg agcgttaggg gcagcatttt
    12001 caaccaacgg accgccgatg gttatctacg gtatgctgcg taacttaggc ccggcggcat
    12061 ttcgcggcac tctgaacgct ttcttcaccg caaacaacat cgcggtgatc ggcgggctgg
    12121 cgaccagcgg aattttgact gtctctatct ttaaactggt gctgttttgt attcccacca
    12181 tgatcctcgg ttcactggtt ggacaatatg tgcataaacg catttcggtc aaagtgttcc
    12241 gtgtgatggt ctttttattg ctgattgcct ccggttctat gttgataaaa ggcgcgctgg
    12301 gaattcccgc cgctgccgca cttcttcctc cgtttacctt gttagttata ctgcaactgg
    12361 catttggtaa aaaagtggcg gcaatccgtc gggctgaaga aagtaaataa tcattatgcg
    12421 ggttttaatt tatacccgca cacattcttt aattaatgaa acctgtaata acaggatggc
    12481 atgaaatatt agttttatat ctatggagat tatttatgga tacgccacgc ccacagctaa
    12541 tagattttca atttcatcag aataacgaca gttttactct acgttttcaa gaccgcctta
    12601 ttttaatcca tagcaaagat aatccttgtt tatggattgg ttcaggtata gcggatatcg
    12661 atatgttccg cggtaatttc agcattaaag ataaattgca ggagaaaatt gcgcttaccg
    12721 acgccatcgt cagccagtca ccggatggct ggttaatcca tttcagccgt ggttctgaca
    12781 ttagcgccac gctgaatatc tctgccgacg atcatggccg tttattgctg gaactacaaa
    12841 acgacaacct taaccacaac cgcatctggc tgcgccttgc cgcacagccg gaagatcata
    12901 tctacggctg cggcgaacaa ttctcctact ttgatctgcg cggcaaaccg ttcccgctgt
    12961 ggaccagtga acaaggcgtt ggtcgcaaca aacaaaccta tgtcacctgg caggccgact
    13021 gcaaagagaa tgcgggaggg gattactact ggaccttctt tccacaaccc acgtttgtta
    13081 gcacgcagaa gtattactgc catgttgata acagttgcta tatgaacttc gactttagcg
    13141 cgccggaata ccatgaactg gcgctgtggg aagacaaagc aacactgcgt tttgaatgtg
    13201 ctgatacata catctccctg ctggaaaaat taaccgcgct gctgggacgc cagccagaac
    13261 tgcccgactg gatttatgac ggagtaacgc tcggcattca gggcggcact gaggtgtgcc
    13321 agaagaaact ggacaccatg cgtaacgcgg gcgtgaaggt caacggcatc tgggcgcagg
    13381 attggtccgg tattcgtatg acctcttttg gcaaacgcgt aatgtggaac tggaagtgga
    13441 acagcgaaaa ctacccgcaa ctggattcac gtatcaagca gtggaacaaa gagggcgtac
    13501 agttccttgc ctatatcaac ccgtatgttg ccagcgataa agacctctgc gaagaagcgg
    13561 caaagcgcgg ctatctggca aaagatgccg ccggcggcga ttatctggtg gagtttggcg
    13621 agttctacgg cggcgttgtc gatctcacta atccggaagc ctacgcctgg ttcaaggaag
    13681 tgatcaaaaa gaacatgatt gaactcggct gcggcggctg gatggctgac ttcggcgagt
    13741 atctgcccac cgacacttac ctgcataacg gcgtcagcgc cgaaattatg cataacgcct
    13801 ggcctgcgct gtgggcgaaa tgtaactacg aagcccttga agaaacgggc aagctcggcg
    13861 agatcctctt ctttatgcgc gccggttcca ccggtagcca aaaatattcc accatgatgt
    13921 gggcgggcga ccagaacgtt gactggagtc tggacgatgg actggcgtct gtcgtcccgg
    13981 cggcgctgtc gctggcaatg accggacatg gcctgcatca cagtgatatt ggcggttaca
    14041 ccaccctgtt tgagatgaag cgcagcaaag agctgctgct gcgctggtgc gatttcagcg
    14101 ccttcacgcc gatgatgcgc acccatgaag gtaaccgtcc tggcgacaac tggcagtttg
    14161 acggcgacgc ggaaactatc gcccattttg cccgtatgac caccgtcttc actaccctga
    14221 aaccttacct gaaagaggcc gtcgcgctga atgcgaagtc cggcctgccg gttatgcgcc
    14281 cgctgttcct gcattacgaa gacgatgcac agacctacac cctgaaatat cagtacctgt
    14341 taggccgcga cattctggtc gccccggtac acgaagaagg tcgcagcgac tggacgctct
    14401 atctgccgga ggataactgg gtccatgcct ggacgggtga agcgttccgg ggcggggaag
    14461 tgaccgttaa tgcgcccatc ggtaaaccgc cggtctttta tcgcgccgat agcgaatggg
    14521 cggcactgtt cgcgtcgtta aaaagcatct aatcacatcc gcccgtgggg aaactcacgg
    14581 gcaaagggag aacaacgatg agtcacatca caacggaaga tccagcaact ctacgcctgc
    14641 cctttaaaga gaaactctct tacggtatcg gcgatctggc ctctaacatc ctactggata
    14701 tcggcacgct ttatcttttg aagttttata ccgatgttct ggggctgcct ggcacctatg
    14761 gcggcattat ctttttgatt tcaaaattct ttaccgcctt taccgatatg ggtaccggca
    14821 ttatgttgga ttcccgacgc aagatcggtc caaaaggtaa gttccgtcct tttattctgt
    14881 atgcgtcatt tccggtcacc ctgctggcga tcgccaactt tgtcggcaca ccgtttgatg
    14941 tcaccggtaa aacggtgatg gccaccatcc tgtttatgct ttacggactg tttttcagca
    15001 tgatgaactg ctcgtatggc gcgatggtcc ccgctattac taaaaacccc aacgaacgtg
    15061 catcgctggc agcatggcgt cagggtggcg ctacattggg cctgctgctg tgtacggtgg
    15121 gattcgtgcc ggttatgaat cttatcgagg gtaatcagca acttggctat atcttcgccg
    15181 ccacgctgtt ttcactgttc ggcctgctgt ttatgtggat ctgctattcg ggcgtgaaag
    15241 agcgttatgt cgaaacccaa cctaccaatc cggcgcaaaa gcctggcttg ttgcaatctt
    15301 tccgcgcaat tgcggggaac cgcccactgt tcattttgtg cattgccaac ctctgtacct
    15361 taggggcgtt taacgtcaag ctcgccattc aggtctatta cacccagtac gtgcttaacg
    15421 atcccatcct gttgtcatat atgggatttt tcagcatggg ctgtattttc atcggcgtgt
    15481 tcctgatgcc tggtgcagtc agacgttttg gtaagaagaa ggtctatatc ggcggtctgc
    15541 tgatctgggt gctgggcgat ctgctcaact atttcttcgg cggcggctcg gtcagctttg
    15601 tggctttctc ctgcctggcg ttctttggtt cggcgtttgt taacagcctg aactgggcgc
    15661 tggtttctga caccgtcgag tatggcgagt ggcgcaccgg tgtgcgttcg gaaggtacgg
    15721 tctacaccgg tttcaccttc tttcgcaaag tatctcaggc gctggcaggt ttcttcccag
    15781 gctggatgct gacgcaaatt ggctatgtgc caaacgtcgc ccaggctgac cacactatcg
    15841 aagggttgcg ccaactgatc ttcatctacc caagcgcact ggcagtagtc accattgtgg
    15901 caatgggttg cttctacagt ctgaacgaga agatgtacgt ccgcattgtt gaagagatag
    15961 aagcccgtaa acgcacggcg taattataat aaacaacgcc ctgcggggcg ttataaggag
    16021 tgattatgtc tgaccataat ccactgacat taaaactgaa tctgcgggaa aaaatcgcct
    16081 atggtatggg cgacgtcggt tcgaatttaa tgcttagcat cggcactctg tatctcctca
    16141 aattttatac cgatgaatta gggatgcctg cttactatgg cgggattatc tttctggtgg
    16201 cgaagttttt taccgcattt accgatatgc tcaccggatt tttactcgac tcgcggaaaa
    16261 atattggacc taaaggaaaa ttcaggccgt ttattttata cgcagcgatt ccggcggcgt
    16321 taattgcaac gctccagttt atcgccacca cctttagttt gccggttaaa acgacgattg
    16381 ccaccgcact gtttatgatg tttggacttt catacagtct gatgaactgc tcgtatggcg
    16441 cgatgatccc ggccattacc aaaaacccga atgagcgcgc gcaactcgct gcttaccgcc
    16501 agggtggtgc aaccataggg ctattgatct gtaccgtggc gtttattcct ttgcagtcac
    16561 tattttctga ctccaccgtc ggctacgcct gcgcggcact gatgttctcc attggcggct
    16621 ttatttttat gatgctgtgc tacagaggtg tcaaagagca ttatgtggac acgacgccaa
    16681 ccggacataa ggccagtatt ctcaaatcat tttgcgcgat atttcgtaat ccacctttgc
    16741 tggtattgtg cattgctaac ctgtgtaccc tggcggcatt taatatcaaa ctggcgattc
    16801 aggtctatta cacccagtat gtgctgaatg atattaactt attgtcgtgg atgggattct
    16861 ttagtatggg atgcattctc gtcggcgtat tactggtgcc actaactgta aaatgttttg
    16921 gtaaaaaaca ggtttatctg gctggcatgg tgctgtgggc ggtgggggat atcctgaatt
    16981 attgctgggg cagtaactca ttcactttcg tcatgttctc ttgtgtcgcc ttttttggca
    17041 cggcctttgt aaacagcctg aactgggcac tggtaccaga taccgtagat tatggtgaat
    17101 ggaaaacagg tatacgtgcc gaaggttctg tttataccgg ttataccttc tttcgtaaaa
    17161 tttcagccgc acttgctggc ttcttgcccg gcattatgct gacgcaaatc ggttatattc
    17221 ccaacatcgc gcaaagcgat gcaacattac agggtttgcg tcagctcatt tttatctggc
    17281 cttgtgcgct ggcaattatt gctgcattga cgatggggtt cttttacacg ctcaatgaga
    17341 aacggtttgc actgattatt gaggaaatta accaacgcaa aaataaagaa attgagacag
    17401 aagaaaaaac ggcttccgta acgttataaa tgtcaatagc cgctatttcc atcctgatgg
    17461 gtggcggcct ccctacgttt aaaaaatgga cttattatga aaaatattaa tgcaataatt
    17521 ttattatctt ctctgacctc tgcctcggta ttcgctggcg catacgtcga aaacagggaa
    17581 gcatataatc tcgcctccga tcagggtgag gttatgctac gtatgggtta taacttcgat
    17641 atgggcgcgg gtattatgct aaccaatacc tacacctttc agcgagaaga tgaactcaaa
    17701 catggctata atgaaattga aggttggtat cctttattta aacctaccga taaattaacc
    17761 atccagcccg gtggcttaat taatgacaag agtatcggtt cgggtggtgc tgtttatctg
    17821 gatgttaact acaaatttac tccgtggttt aacctgacgg tacgtaatcg ctataaccat
    17881 aataattaca gctctacaga tttgaacggc gaattggata ataacgacac ctatgaagtt
    17941 ggcacctact ggaactttaa aataacagaa aaattttcct atacatttga accacattac
    18001 ttcatgcgag tgaatgactt taatagtagc aacgggaaag atcatcattg ggaaatcact
    18061 aacaccttcc gttaccgtat taatgaacac tggcttcctt atttcgaatt gcgctggtta
    18121 gaccgcaacg tcgagccgta ccaccgcgag caaaaccaga ttcgtatcgg aacgaagtat
    18181 ttcttctgat gtaaaaattc ctgatgcgat gctggcgcat cttatcaggt ctacaaaagc
    18241 caaaaaaaac ctgccatcgc tggcaggttt tttatgactg aaaaacgaaa ttaatcgtct
    18301 ttcggtgcgc ggttggcgcg gcgacgatca ttttccgtca ggtgacgttt acgaatacga
    18361 atagaggtcg gagtcacttc taccagttcg tcatcatcga tgaactccag agcttgttcc
    18421 agagtcatgc ggataggcgg aaccagaaca acggcttcgt cagtaccgga agcacgcatg
    18481 ttggtcagtt tcttaccggt caggcagttt acagtcaggt cgttagagcg gctgtgaata
    18541 ccgataatct gaccttcgta aacttctgca ccgtgaccga ggaacagctt accgcgatcc
    18601 tgcagaccga acagcgcgaa cgctaccgct ttaccctgac cgttagagat cagtacgccg
    18661 ttctgacgct ggcccacttc acccggacgt acgtcgtcgt agtggctgaa ggtggagtac
    18721 agcagaccag taccggaagt catggtcatg aactcagaac ggaagccaat cagaccacgg
    18781 cttgggatca cgtagtcgag acgtacgcgg cctttaccgt ctggattcat gtttttcagg
    18841 tcgcctttac gttcgcccag cgcctgcatt acagaaccct gatgctgttc ttcaacgtcc
    18901 agcgtcacgt tttcatacgg ctcttgttta cgaccgtcga tttcacggaa gataaccttc
    18961 ggacgggata ccgccagttc gaaaccttca cgacgcatgt tttcgatcag aacagacagg
    19021 tgcagttcgc cacgaccaga aacgcggaac gcatcggcgt cttcggtttc ttctacgcgc
    19081 agcgcaacgt tgtgtaccag ttctttgttc agacgatcca ggatctgacg agacgttacg
    19141 aacttacctt ctttaccgca gaacggcgag gtgttaacgc agaagaacat agaaacggtc
    19201 ggctcatcaa cggagagtgc cggcagcgct tcaacgtttt gcgtgtcgca aacggtgtca
    19261 gaaatgttca gttcgcccag gcccgtgatc gccacgatat cgccagcttc cgccagatcg
    19321 gtttcgatac gttccagacc gaggtggccc agcactttac cgactttcgc gttgcgggtt
    19381 ttgccttcgc tatcgatgat agtaacctgc tggttcggct tcactttacc gcgcttgatg
    19441 cggccaatgc cgataacgcc aacatagctg ttgtaatcga gctgagaaat ctgcatctgg
    19501 aacggaccgt caaggtcaac gtccggcgca ggaacgtggt caacaatcgc ctggtacagc
    19561 ggggtcatgt cttccgccat atcttcgtgg tccagacccg cgataccgtt cagcgcagaa
    19621 gcgtaaacga tcgggaagtc cagctgctcg tcggtcgcgt cgaggttaac gaacagatcg
    19681 aatacctgat ccacaaccca atcaggacgc gcgccagggc ggtcaacttt gttgataaca
    19741 acaatcggct tcaggccgta agcaaacgct tttttggtta cgaagcgcgt ttgcggcatc
    19801 gggccgtcaa atgcgtcaac caccagcagc actgagtcta ccatggacat tacacgttca
    19861 acttcaccac cgaagtcggc gtgccccggg gtatcaacga tgttgatacg gtaatcattc
    19921 catttgatag cggtgttttt cgcgaggatg gtaatcccac gctctttctc caaatcgttg
    19981 gagtccatca cgcgctcttg ggtttcggca cgagagtcga acgtaccgga ttgttggagc
    20041 agcttgtcta ccagggtggt tttaccatgg tctacgtgcg cgatgatggc gatattacgc
    20101 aatttttcga tcacaacttt gcctcaggca ttagaaatag cgcgttattg tacacggatt
    20161 aatcgcacta caaaacagga tcacaaacat cctccgcaaa caagtattgc agagtccctt
    20221 tgtgatcgct ttcacggagc ataaaaaggg ttatccaaag gtcattgcac caatatggtg
    20281 cttaatgttc ccattgaagc actatattgg tgcaacattc acatcatggt gcagcccttt
    20341 tgcacgatgt taagcatgat aacgcctttt aggggcaatt taaaagttgg cacagatttc
    20401 gctttatctt ttttacggcg acacggccca tttaattgca gatttcgtta ccacgacgac
    20461 aatgaccaat ccaggagagt taaagtatgt ccgctgaaca cgtactgacg atgctgaacg
    20521 agcacgaagt gaagtttgtt gatttgcgct tcaccgatac caaaggtaaa gaacagcacg
    20581 tcactatccc tgctcatcag gtgaatgccg aattcttcga agaaggcaaa atgtttgacg
    20641 gctcctcgat tggcggctgg aaaggcatta acgaatccga tatggtgctg atgccagacg
    20701 catccaccgc agtgattgat ccgttcttcg ccgattctac cctgattatc cgttgcgaca
    20761 tccttgaacc tggcaccctg caaggctatg accgtgaccc gcgctctatt gcgaagcgcg
    20821 ctgaagatta cctgcgttct actggcattg ccgacaccgt actgttcggg ccagaacctg
    20881 aattcttcct gttcgatgac atccgtttcg gatcgtctat ctccggttcc cacgttgcta
    20941 tcgacgatat cgaaggtgca tggaactcct ccacccaata tgaaggtggt aacaaaggtc
    21001 accgtccggc agtgaaaggc ggttacttcc cggttccgcc ggtagactca gctcaggata
    21061 ttcgttctga aatgtgtctg gtgatggaac agatgggtct ggtagttgaa gctcatcacc
    21121 acgaagtcgc taccgcaggt cagaacgaag tggctacccg cttcaatacc atgaccaaaa
    21181 aagctgacga aattcagatc tacaaatatg tcgtgcacaa cgtggcgcac cgtttcggta
    21241 agaccgcgac ctttatgcca aaaccgatgt tcggtgataa cggttccggt atgcactgcc
    21301 acatgtcgct gtctaaaaac ggcgtcaacc tgtttgctgg cgacaaatac gcaggtctgt
    21361 ctgaacaggc actgtactac attggcggcg taatcaaaca cgctaaagcg attaacgccc
    21421 tggcaaaccc gaccaccaac tcttacaagc gtctggtccc gggctacgaa gcaccggtaa
    21481 tgctggctta ctctgcgcgt aaccgttctg cgtctatccg tattccggtg gtttcttctc
    21541 cgaaagcacg tcgtatcgaa gtacgtttcc cggacccggc ggctaacccg tacctgtgct
    21601 ttgctgccct gctgatggcc ggtcttgatg gcatcaagaa caagatccat ccgggcgaag
    21661 ccatggacaa aaacctgtat gacctgccgc cagaagaagc gaaagagatc ccacaggttg
    21721 caggctctct ggaagaagca ctgaacgaac tggatctgga tcgcgagttc ctgaaagccg
    21781 gtggcgtgtt cacggatgaa gcgatcgatg cgtacatcgc tctgcgtcgc gaagaagatg
    21841 accgcgtgcg tatgactccg catccggtag agtttgagct gtactacagc gtctaatagt
    21901 tgaagttgta ctacccggcg caacaacgcc gggatttagt tgccgtggaa actttcagcc
    21961 catctctgca tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta
    22021 aaaagctata atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg
    22081 cacgcagccc gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaatcga
    22141 tgacaacctg gcgatccatt acgccaaccc cgccgcgcaa caactgctcg cccaaagctc
    22201 ccgcaaattg tttggtacac cgttaccgga actgttgagc tacttctcat taaatatcga
    22261 gctgatgcaa gaaagtctgg aggcggggca aggttttacc gataacgaag tgacgctggt
    22321 catcgacggg cgctcgcata tcctttctgt gacggctcag cgtatgccgg acggcatgat
    22381 cctgctggag atggcaccga tggataacca gcgccgctta agtcaggaac agctacagca
    22441 cgcccagcag gttgctgccc gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc
    22501 gcttggcggt ttacgtggcg cggcgcagct actcagcaaa gcattacctg acccgtcact
    22561 actcgaatat accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatc tggtcgaccg
    22621 tctgttgggg ccgcagctac ccggtacgcg cattaccgaa agtattcaca aagtggctga
    22681 acgcgtggtg acgctggtgt cgatggaact gccgaacaac gtgcggttga ttcgggatta
    22741 cgaccccagc ctgccggaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa
    22801 tattgtgcgc aatgcgctac aggcgctggg gccggagggt ggtgaaatca ttctgcgtac
    22861 ccgcacagcg tttcaactga ccttacacgg cgagcgctat cggctggcgg cgcggattga
    22921 tgtggaagat aacgggccgg gcattccgcc tcatttgcag gatacgctgt tttacccgat
    22981 ggtcagcggc cgcgaaggtg gcaccgggct tggcttatcc atcgcccgta atttgattga
    23041 tcagcattca ggcaaaattg aatttaccag ttggccaggt cataccgagt tctcggttta
    23101 cctgcctatc aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat
    23161 gacgatagtt ccatccgttg ggtgcttgaa cgtgcgctcg ctggagctgg tttaacctgt
    23221 acgacatttg agaacggcgc ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg
    23281 ctgctttcag atatccgtat gccgggaatg gacgggctgg cgctgctcaa gcagattaaa
    23341 cagcgccatc cgatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc
    23401 gtcagcgcct atcaacaagg ggcatttgat tatctgccca aaccgtttga tatcgacgaa
    23461 gccgtggcgc tggttgagcg cgccatcagt cattaccagg aacagcagca gccgcgtaat
    23521 gttcagctta acggcccaac gaccgatatc atcggtgaag cgccagccat gcaggacgtg
    23581 ttccggatta tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgaatcc
    23641 ggcaccggta aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg agccaaagcg
    23701 ccgtttatcg cgctcaatat ggcggctatc ccgaaggatt tgatcgaatc agaactgttt
    23761 ggtcacgaga aaggcgcatt taccggcgcg aataccattc gtcaggggcg ttttgaacag
    23821 gctgatggcg gtacattatt tctcgatgaa attggcgata tgccgctgga tgtgcagacg
    23881 cgtttgctgc gcgtgctggc agacggtcag ttttatcgcg ttggcggcta tgcgccggtg
    23941 aaagtagatg tgcggattat cgctgccact caccagaatc ttgaacagcg ggtgcaggaa
    24001 ggcaagttcc gtgaggatct gttccaccgc ctgaacgtca tccgcgttca tctgccgcca
    24061 ctgcgcgaac gtcgggaaga tattccccgt ctggcacgcc attttttaca ggttgccgcg
    24121 cgagagctgg gcgtagaagc gaagttgctg catccggaaa ccgaagccgc gctgacgcgt
    24181 ctggcgtggc caggcaacgt gcgccagctg gaaaacacct gtcgctggct aacggtgatg
    24241 gccgccgggc aggaagtgtt gattcaggat ttgcctggcg aactgtttga atcaaacgta
    24301 ccggaaagca cttcacacat gcagccggac agttgggcaa cgctgttagc acagtgggca
    24361 gacagagcgc tgcgttccgg tcatcaaaac ctgctttccg aagcacagcc agagctggaa
    24421 cggacgttac tggccaccgc gctgcgacat acgcaggggc ataaacagga agcggcgcgg
    24481 ctacttggct ggggccgcaa caccctgacg cgtaagttaa aagagctggg gatggagtga
    24541 ttcacggctt gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttgcgcttta
    24601 ctgttccgat aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa
    24661 tcaataatta atctggtatc gagtggcgca gttgacagcc acacaccgca aactgctgtt
    24721 gctgccgtgc tgtgtgccgc gatgataggg ctgtttagct gaagctcgtt gtttacgccg
    24781 gatgcggaga aagtcgtagg cctgataaga cgcaaccagc gtcgcatcag gcgttgattg
    24841 ccggatgcgg cgtaaacgcc ttatccggcc tacaaaagca ataatttcaa cagattttgc
    24901 aggcctgata agcgtagcgc atcaggcaat cttgttctca gcacattgat ggcgctttcg
    24961 ttttctactt tgtaaacgaa gcgccattca caatttaaat cacccgagag aactgctgca
    25021 tccgcgcttt ctggcgcaga taggtatcaa agcacatgca aatgttgcgg atcagcaagc
    25081 gacctttcgc cgtcacctgt attcccttct catccacatc caccagccca tcttttgcta
    25141 acggggcgag cagcttgaga tcttccgcaa agtaatcagc gaagagcaaa tcccactgtt
    25201 gctcaatagg ggcgtaatcc agacggaagt tgcagatgag cgacttaatc acatcgcggc
    25261 gaatacagtc atcacgcgtt agcgcaatac cacgccacag cgcattgcct tgttcatcca
    25321 cttgctgata gtactgcttc aactctttct ggttctgcgc gtagcagtcg ccaatcatgc
    25381 tgatggcgga aacgcccatc ccaagcagat cggtatcgcc ctgggtggtg tagccctgga
    25441 agttacgatg cagcacgcct tcacgctggg ccaccgccag ctcgtcatcc gggcgagcaa
    25501 agtgatccat accgataaac tgatagcccg attgtgtcag gaaggcgatg gtttcctgca
    25561 gaatatcgag tttttgttgc ggactcggca agtcagcatc tttgattttg cgctgggcgg
    25621 caaaaatagt tggcaaatgc gcgtagttaa agacgctcag acgatcgggg ttcagctccg
    25681 ccacgcgttt cagggtaaag gcgaaactct ccggcgtctg tttcggcagg ccgtaaatca
    25741 ggtcgatgtt ggtggaggta aagccaatct cacgcgcatg gttaagcagt gcaaagatga
    25801 actcttcatc ctgctcgcgg ttaaccagac gttgcacttc tttgttgaag tcctgcacgc
    25861 ccatgctcag ccgattaaag ccttcggcgc gtaaatgatc gagtacatcc agttcgattt
    25921 cccgcggatc gacttcgatc gagatctccg catcggcatt gaactggaag ttttcgcgca
    25981 gcagcttcat cagacggctg atttgcgctt tattcagata cgtcggcgtt ccgccgcccc
    26041 agtgcaattg gctgacgtga cgcccggcga acagcggtgc acgatggacg atttcttgct
    26101 ccagcgcgtc cagatactga tcggccttgt gctgctggcg agtaacaatc ttattgcaac
    26161 cgcagaagta acaaagctta tggcagaacg gaatatgtac gtagagagat aatggacgct
    26221 caggatagcg cgccacggct tgtaaaaacg cctgttcgcc gaagtcttct gaaaactcca
    26281 gcgcggtcgg gtacgaggtg tatcgtggcc cggaatagtt atatttctgg atcagggcca
    26341 gatcccagtc gatttgctgt acagacatgc tcactccttc cgatggcgtc tctggcggct
    26401 acggcgtacc ggcggatgcc cgttgcgagt catcagccgg ttgcgcagcc acttttgtcg
    26461 ccgcgacaac cgctgtagtt taacgaataa ccacaccaga taacatataa ccggaagggt
    26521 tatcagcaga acgggaaggc ccacggcggc aatccgttag ttgccccgca gcagacgcat
    26581 catgtcttct tgcttctcgt cttcttcctc ttcttcgtca tcgtcataag agaggccgag
    26641 tttctgcatc aactcatcaa tacgatccag tttggcatcc acccaggatt gctcttcggc
    26701 gctcagggtt tcgcctgctt ccagacgttc cagcagcgca tccagacgct catccgtttc
    26761 cagtaactcc aactccgcct gcggtgaaag cataggtttc tcactcttcg gtttgtgctg
    26821 tttggtgact ttttcagtca cgcccaatgg aataggggtt ttactgccaa tacgtggatc
    26881 ttttggtgcg ttctgacctt tgctgcctga cgtggtgtta ccgcccgctg cgcggctgcc
    26941 cggcgcatga ccacgacgtt ttttctgacg cttacggtca cgagcttcct gatccagctc
    27001 ctcgcgtgtt tttcgacgcg cttttgcatg acctttgctg cgtgagtttg aagatgatgg
    27061 tttcataatg gttcgtcttt tagccgtttt attcagtata gatttgcggc ggaatctaac
    27121 agaaagcaag caaagaaaaa aggcgacaga ttactctgtc gccttttttc ctgactcata
    27181 acccttaacg ggtcttacaa tccttgcagg ccgccaacac accctgtttt tccattagcc
    27241 ggaacaatcc gtgttgattc catttccatt taattacgtc tccggacgtt tatcttcctg
    27301 acaatcctct gtcttcgcct cctggcgctc ctgaccctca tcctgagtca ttgttcctgt
    27361 tagcgtcctc gctctatggg tgctacttta cgcctttgct ttaaataaac aatcaaccgc
    27421 catcagtatt tgcacatttt cagagtttta tataaaagca attcactgat ttatatagct
    27481 tatttgacat acgtcgcaga attttctctg ctatttcgca taaagactat cggcaatgtc
    27541 tcacaaagcg tatggctttt acttacaacg ctcacgccga aaaatccgcc ttttattatg
    27601 gttcaggtat aatcgccgca aagctttgac tgatggagac gaccgctttg actaatttga
    27661 attatcaaca gacgcatttt gtgatgagtg cgcctgatat tcgccaccta ccttccgata
    27721 ccggaattga agtggctttt gcaggccgtt ccaacgcagg taaatccagc gcgctgaaca
    27781 cgctgactaa ccagaaaagc ctggctcgta cctcaaaaac cccggggcgc acccagctta
    27841 tcaacctgtt tgaagtggct gacggcaagc gtctggttga cttgcctggg tacggttatg
    27901 cggaagtccc ggaagagatg aagcgcaaat ggcagcgcgc gctcggcgag tacctcgaaa
    27961 aacgtcagag cctgcaaggt ctggtggtgc taatggatat tcgccatccg ctgaaagatt
    28021 tggatcagca gatgattgag tgggcggtag acagcaatat cgccgtgctg gtactgctga
    28081 ccaaagcgga caaactggca agcggcgcac gtaaagcgca attgaatatg gtgcgtgaag
    28141 cggtgctggc gtttaacggt gatgtgcagg ttgaaacgtt ttcttcgctg aagaaacaag
    28201 gcgtggacaa gctgcggcag aaactggata cctggtttag cgagatgcag cctgtagaag
    28261 aaacgcagga cggcgaataa ttttcttgcc ttaatgcttg tgccggatgt ggcgtatccg
    28321 gcccgtaaat tcaatagatt acaattctac cgttggcctg ataaacatgg cgtatcaggc
    28381 attacggatc ttttctttcg cccaataaaa aacgccccag tcattactga ctggggcggc
    28441 taaaatattc agccaaatcc gattacgtga agtaaaaggt ctgaaagata gaacatctta
    28501 cctctgtacc ctacgcgact aactttactc ttttttgttc agttcagaaa gcattttttg
    28561 taattttttt tcagttgttg cataggaatt tctaatagcc atcacaaaac gtgacagctt
    28621 atgttgctta cttacgaaaa aagggcatgt tcaggcgaat cttagtgcgc ctgatcccag
    28681 ttttcgccac tccccacttc caccagtaac ggcacatcca gacgggtaca gttttccatc
    28741 agttgatgaa tctgcttcgc gacggcatcg acatcatctt tatggacttc aaataccagt
    28801 tcatcgtgta cctgcatgat catacgcacg cgcggttgct cagcctgtag ccacgcatca
    28861 acggcaatca tcgcccgttt gataatgtcg gcggcggttc cctgcattgg cgcgttaatg
    28921 gctgcacgtt cagccgctgc acgacgagca ccattgctgg atttgatatc cggcagatac
    28981 agacggcgtc cgtccagcgt ttcaacgtag ccctgctctt tcgcctgagc acgggtgcgt
    29041 tccatatact ccagcacgcc agggtagcgt tcgaagtaaa ggtccatgta cttctgcgct
    29101 tctttacgcg gaatgttcaa ttgccgcgcc agaccgaaag cactcatgcc ataaatcaga
    29161 ccaaagttga tcgcttttgc actacgacgt tgctcgctgg tgacggtttc cagtggcaaa
    29221 ccaaacactt ctgccgccgt tgcacggtgg atatcttttc cttccgcgaa tgcggtcagc
    29281 aagcctttgt cacgcgagag atgcgccata atgcgcagtt caatctgcga gtagtccgct
    29341 gagacaatca cataatcctc tggcgcaata aacgcctggc ggatacggcg accttcttcg
    29401 ttacgcaccg gaatgttttg caggttagga tcggttgacg ataaacgtcc cgttgcggtt
    29461 actgcctggt gataagaggt atgtacacgc ccggttttcg ggttgatcat caacggcagc
    29521 ttgtcggtgt aggtcgattt cagcttcgcc agaccacgat actccagaat cacttttggc
    29581 aacggatagt ccagcgccag ttcttccagt acctcttccg acgttgatgg cgcgccaccc
    29641 ggcgttttct tcagtggttt aatgccctgt ttttcaaaca gaatggtttg taactgcttg
    29701 gtggaagaaa ggttaaattc ttcacctgca atttcatgag ctttcttttc cagctcagcc
    29761 agacgcaagg tgagctcttc agagtgattg tgcagcactt tcggatcgat cttcacaccg
    29821 ttacgttcaa tgcgtgaaag caccggcacc agcggcattt cgatattctc gaagacgttc
    29881 aacggccctt tgtgtttttg cagatccggc cacattttca gatgcaactg caaggtgaca
    29941 tctgcatctt cggcggcgta acgtccggct tcttcgaggg caatctggtt aaaggtcagt
    30001 tgatttttgc ctttacccgc aatctcttca aaagtgatgg ttttgtgctt caaccaacgt
    30061 tccgcgaggc tgtccatatc gtgacgtccg gcaacgctat tgagaatgta ggactccagc
    30121 atggtatcaa acgcaatccc acgtagttca atgccgtagt tcgccagaat accgcgatcg
    30181 tatttcaggt tttgcccgac cttcagcgcc ttttcatctt ccagcagcgg ttttagcaac
    30241 tctagtgcac gctcgcgaga gatttgatcg ggcgcatcaa gataatcatg agcaaccgga
    30301 atatatgccg ctacgcctgg ctcgatagca aaagaaagcc cgaccaggtt agcagagatg
    30361 ttatcaaggc tgtcggtttc ggtatcaaat gcaaataccg gcgctttttc cagcttcgca
    30421 atccactctt tcagtgtttc ttcatcaagg atggtgacgt agttgtcata agaaatcacc
    30481 gttgccgtca cttctggcgc ttcgtctgca acactggttt cctgcggcct ggctgcgggt
    30541 ttcgcccctt tagcctgtag ccattttccc gcttcgacat cagcagtcca gcgtttgaac
    30601 tcatactttt tgaacagacc taacaactct tccgccgccg gtggctgcac ttccagttgt
    30661 tcacaggtca gctccagttc aacgtcggtt ttaatcgtcg ccagctggta tgagagataa
    30721 gcaacttctt tgttttgctc gagcttcgct gccattgttt tcgcgccacg gaagctcaac
    30781 ccagcaattt tttctggctc ggcatacagc gtatccagcc cgccaagacc ttgcagcaat
    30841 gcctgcgcgg ttttttcacc gacgcccggt acgccaggaa tgttatcaga ggagtcaccc
    30901 atcagcgcca ggaaatcgat gatcagttct ggcggcacgc cgtacttatt caccacctct
    30961 tccggtccga ggatggtatt cgtcatggtg ttgataagcg tgatatttgg tgtcaccagc
    31021 tgcgccatat ctttatcgcc agtgctgatc agcaccggac gcccggcttt ttcggcttcg
    31081 cgcgccagag taccaataac gtcgtccgct tctacgccag aaaccgccag cagcggcagt
    31141 cccatcgctt taaccatcgc gtgcagaggt tcgatttgtg ctcgcagatc gtccggcatt
    31201 ggcgggcgat gtgatttgta atgttcaaac agttcatcac gaaaggtttt tcccttggcg
    31261 tcaaagacca ccgctgcatg cgtcggttta tattgcatga tcagactgcg cagcatgttg
    31321 aggacaccat acatcgcacc ggtcggctcg cctgcgctgt tagtcagcgg gggaaacgcg
    31381 tgatatgcgc gataaaggta agatgaacca tctacaagga taagtggatt ttgggggatc
    31441 tgaaccataa tgtccgtgcc tgtttatcag attatgggta aaggatgcca cagacaggat
    31501 gaaaacatga gtttttcacc gcttttcgtg aaaatctttt gtagatcttc tggatcgctc
    31561 gcaaatcgtc atgtggataa ctttgtgcat aaattattta catcgctttt aatttattat
    31621 catgcttttt acagcatgat gtttgtcttt gtttttcatg cagttaaaaa taaagccatc
    31681 cctaataatt gagataatgg gattttttct aatgtggata gatatgaatt atttttctcc
    31741 ttaaggatca tccgttattt gggtcgtttt ctgctaaaat ctgcgccttg cgcttcttta
    31801 gcgcactgtc tatggctaac cttttgaata aattcattat gacgagaata ctcgctgcga
    31861 taaccctttt gctgagtatc gtattgacca ttttggtcac tattttttgt tccgtcccga
    31921 tcatcattgc cgggattgta aaactattgc tacctgtgcc agtcatttgg cgaaaggttt
    31981 cgcgtttttg tgattttatg atgtattgct ggtgcgaagg tctggcggta ttactgcatc
    32041 tgaacccaca cctgcaatgg gaagttcacg ggctggaagg gttaagtaag aagaactggt
    32101 atctgcttat ttgtaaccac cgtagttggg cagatattgt cgtactgtgc gtgctgtttc
    32161 gtaagcacat tccgatgaat aaatatttcc tcaagcagca gctggcctgg gtgccttttc
    32221 ttggcctggc gtgctgggcg ctggatatgc cgtttatgaa gcgttattcc cgcgcttatt
    32281 tattacgtca tccggagcga cgtggtaaag atgttgaaac cactcggcgt tcttgtgaaa
    32341 agtttcgcct gcatcccacc actattgtca atttcgttga aggctcccgt ttcacgcaag
    32401 aaaaacatca gcaaacccac tccacttttc aaaacttgtt gccaccaaag gcggcaggca
    32461 ttgcaatggc gctaaatgta ctgggtaaac aattcgataa actgcttaat gttacgctgt
    32521 gttatccgga caataaccgt cagccgttct tcgatatgtt aagc
//
LOCUS       DAZFFE010000046        30705 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFFE010000046 DAZFFE010000000
VERSION     DAZFFE010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.046"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..706
                     /gene="lhgO"
                     /locus_tag="ACHJ69_002328"
     CDS             <1..706
                     /gene="lhgO"
                     /locus_tag="ACHJ69_002328"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO5015303.1"
                     /translation="GEYEASTLISCSGLMADRLVKMLGLEPGFIICPFRGEYFRLAPE
                     HNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSMTVGPNAVLAFKREGYRKRDFSFSDTL
                     EILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLSLSDLQPWPAGVRAQ
                     AVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSKVQTLLASQSNPGRT
                     LRAARSVDALHAAFNQ"
     gene            729..2177
                     /gene="gabD"
                     /locus_tag="ACHJ69_002329"
     CDS             729..2177
                     /gene="gabD"
                     /locus_tag="ACHJ69_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO5015304.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNFMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQNGVYDRFAEKLQQAVSKLHIGNGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIIGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            2191..3471
                     /gene="gabT"
                     /locus_tag="ACHJ69_002330"
     CDS             2191..3471
                     /gene="gabT"
                     /locus_tag="ACHJ69_002330"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO5015305.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMLRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANVLGQKLKDG
                     LLAIAKKHPEIGDVRGLGAMIAIELFEDSDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            3708..5108
                     /gene="gabP"
                     /locus_tag="ACHJ69_002331"
     CDS             3708..5108
                     /gene="gabP"
                     /locus_tag="ACHJ69_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO5015306.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVLSTGLLAIGIICTVPIMAR
                     WKKLIMWQKTPIHNTR"
     gene            5129..5791
                     /gene="csiR"
                     /locus_tag="ACHJ69_002332"
     CDS             5129..5791
                     /gene="csiR"
                     /locus_tag="ACHJ69_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO5015307.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGSDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVTGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHNQHQILTEAVLARDAARASELMRQHLLTPIPIIQQAMA
                     CN"
     gene            complement(5792..6241)
                     /gene="kbp"
                     /locus_tag="ACHJ69_002333"
     CDS             complement(5792..6241)
                     /gene="kbp"
                     /locus_tag="ACHJ69_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO5015308.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTAIPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(6325..6483)
                     /locus_tag="ACHJ69_002334"
     CDS             complement(6325..6483)
                     /locus_tag="ACHJ69_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO5015309.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            6666..6965
                     /locus_tag="ACHJ69_002335"
     CDS             6666..6965
                     /locus_tag="ACHJ69_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO5015310.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            6975..7499
                     /gene="ygaP"
                     /locus_tag="ACHJ69_002336"
     CDS             6975..7499
                     /gene="ygaP"
                     /locus_tag="ACHJ69_002336"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO5015311.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSVFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(7546..7950)
                     /gene="stpA"
                     /locus_tag="ACHJ69_002337"
     CDS             complement(7546..7950)
                     /gene="stpA"
                     /locus_tag="ACHJ69_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO5015312.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFIDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            8617..9066
                     /gene="alaE"
                     /locus_tag="ACHJ69_002338"
     CDS             8617..9066
                     /gene="alaE"
                     /locus_tag="ACHJ69_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO5015313.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILVYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(9103..9447)
                     /locus_tag="ACHJ69_002339"
     CDS             complement(9103..9447)
                     /locus_tag="ACHJ69_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO5015314.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            9599..9928
                     /locus_tag="ACHJ69_002340"
     CDS             9599..9928
                     /locus_tag="ACHJ69_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO5015315.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(9985..10728)
                     /locus_tag="ACHJ69_002341"
                     /pseudo
     CDS             complement(9985..10728)
                     /locus_tag="ACHJ69_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            10818..11249
                     /locus_tag="ACHJ69_002342"
     CDS             10818..11249
                     /locus_tag="ACHJ69_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO5015316.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELVYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTEIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            11458..11703
                     /gene="nrdH"
                     /locus_tag="ACHJ69_002343"
     CDS             11458..11703
                     /gene="nrdH"
                     /locus_tag="ACHJ69_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO5015317.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            11700..12110
                     /gene="nrdI"
                     /locus_tag="ACHJ69_002344"
     CDS             11700..12110
                     /gene="nrdI"
                     /locus_tag="ACHJ69_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO5015318.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            12083..14227
                     /gene="nrdE"
                     /locus_tag="ACHJ69_002345"
     CDS             12083..14227
                     /gene="nrdE"
                     /locus_tag="ACHJ69_002345"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO5015319.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     VQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            14237..15196
                     /gene="nrdF"
                     /locus_tag="ACHJ69_002346"
     CDS             14237..15196
                     /gene="nrdF"
                     /locus_tag="ACHJ69_002346"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO5015320.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFASRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNQEKISQAQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWSDDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            15552..16754
                     /gene="proV"
                     /locus_tag="ACHJ69_002347"
     CDS             15552..16754
                     /gene="proV"
                     /locus_tag="ACHJ69_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO5015321.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKAALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            16747..17811
                     /gene="proW"
                     /locus_tag="ACHJ69_002348"
     CDS             16747..17811
                     /gene="proW"
                     /locus_tag="ACHJ69_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO5015322.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            17868..18860
                     /gene="proX"
                     /locus_tag="ACHJ69_002349"
     CDS             17868..18860
                     /gene="proX"
                     /locus_tag="ACHJ69_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO5015323.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            19052..20239
                     /locus_tag="ACHJ69_002350"
     CDS             19052..20239
                     /locus_tag="ACHJ69_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5015324.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASMLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDA
                     RNRLTAGYMTSYFIGGAAGSLISASAWQHGSWAGVCLAGATIALVNLLVWWRGFHRQE
                     AAN"
     gene            20363..21100
                     /gene="ygaZ"
                     /locus_tag="ACHJ69_002351"
     CDS             20363..21100
                     /gene="ygaZ"
                     /locus_tag="ACHJ69_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO5015325.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            21090..21425
                     /gene="ygaH"
                     /locus_tag="ACHJ69_002352"
     CDS             21090..21425
                     /gene="ygaH"
                     /locus_tag="ACHJ69_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO5015326.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            21516..22046
                     /gene="emrR"
                     /locus_tag="ACHJ69_002353"
     CDS             21516..22046
                     /gene="emrR"
                     /locus_tag="ACHJ69_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO5015327.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            22173..23345
                     /gene="emrA"
                     /locus_tag="ACHJ69_002354"
     CDS             22173..23345
                     /gene="emrA"
                     /locus_tag="ACHJ69_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO5015328.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHTRDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            23362..24900
                     /gene="emrB"
                     /locus_tag="ACHJ69_002355"
     CDS             23362..24900
                     /gene="emrB"
                     /locus_tag="ACHJ69_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO5015329.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESLHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            25158..25865
                     /locus_tag="ACHJ69_002356"
     CDS             25158..25865
                     /locus_tag="ACHJ69_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HFO5015330.1"
                     /translation="MNTQRKYGRTWHYPFSPGTTSDDRINTDYWQDLKAITQLVHTEK
                     LDGENNCLNRYGVFARSHATPTQSAWTYKIRQRWQLLKNDLGDLELFGENLYAVHSIE
                     YRALEQDFYLFAVRCQDMWLSWEEVQFYAALFDFPCVPEISGPQPGNDEKSWQRDFLA
                     LTNARGTFDPWDTQTCQPCTLEGIVSRNHDAFSVADFSHNVFKYVRKNHVKTTVHWKR
                     HWQRARMAHEFVYGEQS"
     gene            25862..26974
                     /locus_tag="ACHJ69_002357"
     CDS             25862..26974
                     /locus_tag="ACHJ69_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5015331.1"
                     /translation="MIWQLTDDKRWSALRQRFSWVEEMHHTPQDPEHHGEGDVGVHTE
                     MVLNALITLPEFQQLPAQQQEVLWAAALLHDVEKRSTTVQENGRIQSPGHARRGELTA
                     RQILWRDIPTPFVLREQIVALVRLHRLPLWLLERPEPERLLLAAAMRIDTRLLALLAR
                     ADLLGRQSPDQQSMLERIDLFELFCHEQQCWGKMRPFVSDSARWHYLTHEQSSPDFVP
                     WEAEPFEVILLCGLPGMGKDRYINEQCQGMDVISLDDMRRRINASPDDKTATGRIVQQ
                     AKEEARVFLRQKKPFIWNATNITRQLRSQLISLFTAYGARVKIVYLEVPWAQWKQQNA
                     RREYAVPEAVVMRMASRLEVPQPDEAHSVEYRMTDR"
     gene            complement(27025..27540)
                     /gene="luxS"
                     /locus_tag="ACHJ69_002358"
     CDS             complement(27025..27540)
                     /gene="luxS"
                     /locus_tag="ACHJ69_002358"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO5015332.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARNILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(27690..29246)
                     /gene="gshA"
                     /locus_tag="ACHJ69_002359"
     CDS             complement(27690..29246)
                     /gene="gshA"
                     /locus_tag="ACHJ69_002359"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO5015333.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(29319..29747)
                     /locus_tag="ACHJ69_002360"
     CDS             complement(29319..29747)
                     /locus_tag="ACHJ69_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO5015334.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGVSHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(29744..30310)
                     /gene="yqaB"
                     /locus_tag="ACHJ69_002361"
     CDS             complement(29744..30310)
                     /gene="yqaB"
                     /locus_tag="ACHJ69_002361"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO5015335.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGYYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(30591..30667)
                     /locus_tag="ACHJ69_002362"
     tRNA            complement(30591..30667)
                     /locus_tag="ACHJ69_002362"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30631..30633),aa:Arg,seq:acg)
BASE COUNT         7301 a         7937 c         7889 g         7578 t
ORIGIN      
        1 cggtgaatat gaagcatcga cgctgattag ctgttccggg ctgatggctg accggctggt
       61 gaaaatgctc ggcctcgaac cgggctttat tatctgcccg ttccgtggcg agtatttccg
      121 ccttgcgccg gagcataacc agattgttaa ccacctgatt taccccattc ccgaccccgc
      181 gatgccattt ttgggcgtgc atctcacccg catgatcgat ggcagcatga ccgtcgggcc
      241 aaacgcggtg ctggctttta aacgcgaagg ctatcgcaag cgcgatttct cgtttagtga
      301 cacgctggag attttaggct cgtcggggat tcgccgggtg ctgcaaaacc atctacgctc
      361 aggattgggc gagatgaaaa actcgctgtg caaaagcggc tatctgcggc tggtgcaaaa
      421 gtattgtccc cggctttcgt taagcgatct ccagccctgg cccgcaggtg tgcgggcgca
      481 ggcggtatcg ccggacggca agctgattga cgattttctg tttgtcacca ccccgcgcac
      541 gatccacacc tgcaatgcgc cctccccggc agcgacatca gcaattccta ttggtgcgca
      601 tattgtcagc aaggtacaaa cgctgttggc aagccagagt aaccccggac gcacgctgcg
      661 agcggcacgt agtgtggatg ccttacacgc cgcatttaat caataacctt tgaaaacagg
      721 acgtaacaat gaaacttaac gacagtaact tattccgcca gcaggcgttg attaacgggg
      781 aatggctgga cgccaacaat ggcgaggtca tcgacgtcac caatccggcg aacggcgaca
      841 agctgggtag cgtacccaaa atgggcgctg atgaaacccg cgccgctatc gacgccgcca
      901 accgcgctct gcccgcctgg cgtgcgctca ccgccaaaga acgcgccaac attctgcgca
      961 actggttcaa ttttatgatg gagcatcagg acgatttagc gcgcctgatg accctcgaac
     1021 agggcaaacc actggctgaa gcgaaaggcg aaatcagcta cgccgcctca tttattgagt
     1081 ggtttgcaga agaaggcaaa cgtatttatg gcgacaccat tcccggtcat caggccgata
     1141 aacgcctgat tgttatcaag cagccgattg gcgtcaccgc ggctatcacg ccgtggaact
     1201 tcccggcggc gatgattacc cgtaaagccg gtccggcgct ggcggcaggc tgcacgatgg
     1261 tgctgaagcc cgccagtcag acgccgttct ctgcgctggc gctggcagaa ctggcgattc
     1321 gcgcgggcgt tccggctggc gtgtttaacg tagtcaccgg ttcggcgggc gcggtcggta
     1381 acgaactgac cagcaacccg ctggtgcgca agctgtcgtt taccggctcg accgaaattg
     1441 gccgtcagtt aatggaacag tgcgcgaaag acatcaaaaa agtgtcgctg gagctgggcg
     1501 gcaacgcacc gtttattgtc tttgacgatg ccgatctcga taaagccgtg gaaggcgcgc
     1561 tggcctcgaa attccgtaac gccgggcaaa cctgcgtctg cgccaaccgc ctgtatgtgc
     1621 agaacggcgt gtatgaccgc tttgccgaaa aattgcagca ggcggtgagc aaactgcaca
     1681 tcggcaacgg gctggagaaa ggcgtcacca tcgggccgct gatcgatgaa aaagcggtag
     1741 caaaagtgga agagcatatt gccgatgcgc tggagaaagg cgcgcgcgtg gtttgcggcg
     1801 gtaaagcaca tgaacgcggc ggtaacttct tccagccgac cattctggtg gacgttccgg
     1861 ctaacgccaa agtatcgaaa gaagagacgt ttggccccct cgccccgctg ttccgtttta
     1921 aagatgaagc cgatgtgatt gcgcaagcca atgacaccga atttggcctt gcagcctatt
     1981 tctacgcccg tgatttaagc cgcgtcttcc gcgtgggcga agcactggag tacggcatca
     2041 tcggcatcaa caccggcatt atttccaatg aagtggcccc gtttggcggc attaaagcct
     2101 cgggtctggg tcgtgaaggt tcgaagtatg gcatcgaaga ttacttagaa atcaaatata
     2161 tgtgcatcgg tctttaactg gagaatgcga atgagcagca ataaagagtt aatgcagcgc
     2221 cgcagtcagg cgattccccg tggcgttggg caaattcacc cgattttcgc tgaccgcgcg
     2281 gaaaactgcc gggtgtggga cgttgaaggc cgtgagtatc ttgatttcgc gggcgggatt
     2341 gcggtgctca ataccgggca cctgcatccg aaagtggtgg cggcagtgga agcgcagttg
     2401 aaaaaactgt cgcacacctg cttccaggtg ctggcttacg agccgtatct ggagctgtgc
     2461 gagattatga atcagaaggt gccgggcgat tttgccaaga aaacgctgct ggtcaccacg
     2521 ggttctgaag cggtggaaaa cgcagtgaaa atcgcccgcg ccgccaccaa acgcagcggg
     2581 accatcgcct ttagcggcgc gtatcacggg cgcacgcact atacactagc gctgactggc
     2641 aaggtgaatc cgtactctgc ggggatgggg ctgatgccgg ggcatgttta tcgcgcgctt
     2701 tatccttgcc cgctgcatgg catcagtgaa gatgacgcta tcgccagtat ccaccgaatc
     2761 ttcaaaaatg acgcagcgcc ggaagatatc gccgccatcg tgattgagcc ggttcagggc
     2821 gaaggtggtt tctacgccgc aacaccagcc tttatgctgc gtttacgcgc gctgtgtgac
     2881 gaacacggga tcatgctgat tgccgatgaa gtgcagagcg gcgcggggcg taccggcacg
     2941 ctgtttgcga tggagcagat gggcgtggcg ccggatctca ccacctttgc gaaatcgatc
     3001 gcaggcggct tcccgctggc gggcgtcacc gggcgcgcgg aagtgatgga cgccgtagca
     3061 ccgggcggac tgggtggcac ctatgccggt aacccgattg cctgcgtagc ggcgctggaa
     3121 gtgttgaagg tgttcgagca ggaaaatctg ctgcaaaaag ccaacgttct ggggcagaag
     3181 ctgaaagacg gattgctggc gatcgccaaa aaacacccgg agatcggcga cgtacgcggg
     3241 ctgggggcga tgatcgccat cgagctgttt gaagatagcg atcacagcaa accggacgca
     3301 aaactcaccg ccgaaatcgt ggcacgcgcc cgcgataagg gtctgattct tctctcctgc
     3361 ggcccgtatt acaacgtgct gcgcatcctt gtaccgctca ccattgaaga cgctcagatc
     3421 cgtcagggtc tggagatcat cagccagtgt tttgctgagg cgaagcagta gcaccgcacc
     3481 atgccggagg cgacgctgcg cgtcttgtcc ggcctacgag aatctaggtc ggataaggcg
     3541 tttacgccgc atccgacaat ctgtacgtga acaggaataa atctatgtcg gccggataag
     3601 gcggagccgc tctccggcaa aaagaaccaa taacaattaa acgcgtgacc cgacgcggga
     3661 aatgtcgggg cgctctccca agtgacacac tttcgagagg attcaggatg gggcaatcat
     3721 cgcaaccaca tgagttaggc ggcgggctga agtcacgcca cgtcaccatg ttgtctattg
     3781 ccggtgttat cggcgcaagt ctgtttgtcg gttccagcgt cgccatcgcc gaagcgggcc
     3841 cggcggtatt actggcctat ctgttcgccg gattactggt ggttatgatt atgcggatgt
     3901 tggcggaaat ggcggttgcc acgcccgata ccggttcgtt ttccacctat gccgataaag
     3961 ccattggccg ctgggcgggc tataccatcg gctggttgta ctggtggttt tgggtactgg
     4021 ttatcccact tgaagccaac atcgccgcca tgatcctgca ctcgtgggtt ccaggcattc
     4081 ccatctggtt attttccctc gtcattaccc tcgccttaac cggcagtaat ttattaagcg
     4141 ttaaaaacta cggcgaattt gagttctggc tggcgctgtg taaagtcatc gctatcctgg
     4201 cctttatttt ccttggtgca gtcgcaatta gcggttttta cccgtatgcc gacgtgagcg
     4261 gtatttcaag attgtgggat agcggcggct ttatgcccaa cggcttcggt gcggtattaa
     4321 gcgcgatgtt gatcaccatg ttctcgttta tgggcgcaga aattgtcacc attgccgccg
     4381 cggaatccga cacgccggaa aaacatattg tccgcgccac caactcggtt atctggcgta
     4441 tctctatttt ctatttgtgt tctatttttg tcgttgtggc attaatcccg tggaatatgc
     4501 cagggctgaa agccgttggt tcttatcgct cggttctgga attgctcaat attcctcatg
     4561 cgaaattaat catggactgc gtgatattac tttccgtaac cagctgcctg aactcggcgc
     4621 tgtataccgc ttccaggatg ctctactcct taagccgtcg cggtgatgct cccgcggtaa
     4681 tgggtaaaat caaccgcagt aaaaccccgt acgtggcggt gttactctcc accggcgcgg
     4741 cattcttaac ggtggtggtg aactattacg cgcctgcgaa ggtgtttaaa tttctgatcg
     4801 acagctccgg cgctatcgcc ctgctggttt atttagtcat cgccgtttca caattgcgga
     4861 tgcgcaaaat tctgcgagca gaaggaagcg aaattcgcct gcggatgtgg ctttatccgt
     4921 ggctcacctg gctggtaatc ggttttatta cctttgtgtt ggtagtgatg ctattccgcc
     4981 cggcgcaaca gttagaagtg ctctccaccg gcttattagc gattgggatt atctgtaccg
     5041 tgccaattat ggctcgctgg aaaaagctga taatgtggca aaaaacaccc attcataata
     5101 cgcgctgata atgatcagga gtcacaccat gaccattacc tctctggatg gctatcgctg
     5161 gctgaagaac gatattattc gcggcaattt tcagccggat gaaaaattac ggatgagttt
     5221 gctgacatcg cgttatgcac ttggcgttgg gccgttgcgg gaagcgcttt cgcaactggt
     5281 ggcagaacga ctggtcacgg tggtgaatca aaaagggtat cgggtggcgt ctatgtcaga
     5341 gcaggagctg ctcgatattt tcgatgcccg tgccaatatg gaagcgatgc tggtaagcct
     5401 ggcgattgcc cgcggtagtg atgagtggga ggcagacgtt cttgcgaagg cgcatctgct
     5461 cagtaagctt gaggcctgtg acgccagtga gaaaatgctt gatgagtggg atctgcgtca
     5521 tcaggcgttt catactgcaa ttgtgacagg ctgtggttct cactatttgc tgcaaatgcg
     5581 tgaacggttg tttgatctgg cggcgcgcta tcgcttcatc tggctgcggc gaacggtact
     5641 ttccgtggaa atgctggaag ataaacacaa ccaacatcaa attttaaccg aagctgtgct
     5701 ggcgcgggat gccgcgcgcg ccagtgagtt aatgcgccag catttgctga cgccgattcc
     5761 cattatccag caggcgatgg cttgcaatta actactcttc aggaatacgc aacacttgcc
     5821 ccgggtaaat tttatccggg ctttttagca tcggtttatt cgcttcgaag attttattgt
     5881 acagattagc gttaccgtag acctgtttgg aaatggcact cagagtgtcg ccagacttaa
     5941 cggtataaaa ctggctggca gtggctggta tcgccgtttt cacctgatca tcgacactgg
     6001 caataccgga aatattcccc accgcaacaa ggattttctc cttcgcctcc tgacttaagc
     6061 cgtcaccagt gaccgtcgct ttgccgtcgg caatttgaat gttcacttta tcggcatcag
     6121 gtataccggt tttgttcaga tgctcctgca ccttcttcgc ctgatcgtct ttatcgtgct
     6181 gacctgtaac cgcgtcccag agtttttctc cggcatcttt cacaaaattg aacagaccca
     6241 tagctacctc attcgttaac ggaaacactc agaaagtgta gcagaggaac gcggcgcgtt
     6301 aacgccgcgg gggtgagcac aggtttaatc gcgggtttgc acccagaacg cgtgaatcaa
     6361 accggggata tagccaagca gtgtcagcag aatattaata atgaacgccc aaccgaaccc
     6421 tttaccgagc agcacgccga gcggcggcag aataatggtg atgacgattc tccagaaacc
     6481 catatgtact ccctataaga aaattactca ttgtttaaaa agagatttta tctcttaagc
     6541 gtagtaattt tagcggagcc tgccagtttc agcttacgca tttactctca tttacgcggc
     6601 tgaggctggt atatgttgca tttttagttt agagtttact taatttagaa aatacttaaa
     6661 tatctatgac tgaactcgca caattacagg ccagtgccga acaggcagcg gccttattga
     6721 aagcaatgag ccaccctaaa cggttgctga ttctgtgcat gcttagcggt tcccccggca
     6781 ccagtgcggg agagctgacg cgcattaccg gactaagtgc ctctgcaaca tcacagcatc
     6841 tcgctcgtat gcgggacgag gggcttatcg acagccaacg ggatgcccaa cgcattctat
     6901 attccattaa aaatgaggcg gtaaatgcca ttatcgccac cctgaaaaat gtctattgtc
     6961 cgtaaggagt cactatggct ttgacaacca tttcgccgca tgatgcacaa gaattaatcg
     7021 cacgcggcgc aaagttaatc gatattcgtg atgctgatga atatcttcgt gaacatattc
     7081 ctgaagcaga tctagctcca ttatccgtac tggaacagtc aggtcttccg cctaaattac
     7141 gtcgcgagca aattatattc cactgccagg caggtaaacg caccagtaat aacgccgata
     7201 aattagcagc gattgccgcc cccgcagaaa tctttttact cgaagatggg attgatggct
     7261 ggaaaaaagc aggattgcca gtagcggtaa ataaatccca acccttgccg ttaatgcgcc
     7321 aggtgcagat cgccgcgggt ggtttaatat taatcggcgt tgtactgggt tataccgtaa
     7381 atagcgtttt cttcttatta agcggctttg ttggtgctgg gttactgttt gcaggaatta
     7441 gtggtttttg tggaatggca aggttgttag ataagatgcc gtggaaccaa cgagcttgag
     7501 aagcgacgcc ggacgcgcca tagcagcgac atccggcctc agtaattaga tcaggaaatc
     7561 gtcgagagat ttaccttctg ccagcgcctg agcgattggc ttcggtgtac ggccctgacc
     7621 ggtccaggtt ttagtttcac cgttaacatc gatgaattta tatttcgccg gacgcggctg
     7681 gcgtttttta ccagcgcgtg gtgccgcagc agagctatta cccaataact cttccgggtt
     7741 aattccgtca gctttcatca gctccagcca ggtgctaatt ttttcctggc gctctgccag
     7801 ttcacgctgc tgttgttctt cttcttcacg tctttcttta gtgacaaccc tgaatttttc
     7861 gagcatttct tcaagaacgt caatggagaa ttcgcgagcc atcgcacgga gggtgcgaat
     7921 gttatttaaa ctttgtaaca ttacggacat aataaagaaa accttttaac ccaaaacaaa
     7981 aaagtatttc ttcgcgtagt gtaagcgatc acttattata tttccacaaa atttaatcta
     8041 tttacagtcc tgcacgagat tatttcagct gttattttct gaaactctca gaataatttc
     8101 aagattatat attagccatt ttgatgagta atttcggtta ttggtgaaca gagcggctct
     8161 ctctgctcga taattccgca agcctgacaa aaccaaagtg aataaaaatg caatttaacc
     8221 gaataaaaga aaacatctat cactggtgct ttctgctgtc aatgtacatt atctggcata
     8281 aaaaatgctg aaaatgaccc ggtcactaca tacaccgaat tctctacgct tatagcgtta
     8341 cctcacaccc aaaccttcat gagtgaaaac aaaggcaaac acatatccaa ttgatttaat
     8401 taaaaataaa tcataccggc atgtaaaaat ccacaatgta caaaaaacgc gccgttacgg
     8461 aatattttat ctgcaaaaac agattaaata aaaatttttc actaattgat tagtcatagc
     8521 cagcgatata cgctatgcga aaatgcagat ggcaatgaga tccgctgctt tcatctccat
     8581 taacatccca ttacgctttt attaaggagc attagcatgt tctcaccgca gtcacgcttg
     8641 cgtcatgcag ttgcagatac gttcgcgatg gttgtttact gttctgtcgt gaacatgtgt
     8701 attgaagttt tcctctccgg aatgagcttc gaacagtctt tttattccag attggtagcg
     8761 attccggtga acatcttaat tgcatggcca tacggtatgt accgcgatct gtttatgcgc
     8821 gcggcacgca aagttagccc gtcgggctgg ataaaaaatc tggcggatat cctggtttat
     8881 gtgacgttcc agtcaccggt gtatgtggcg atcttgttag tggtgggcgc agactggcat
     8941 cagattatgg cggcagtcag ttcaaacatc gttgtttcga tgttgatggg ggcggtttat
     9001 ggctacttcc tcgattattg ccgccgactg tttaaagtca gccgttacca gcaggtaaaa
     9061 gcctgaccct tcttttcgcg actggcatcg ccagtcgcgt ctttaactgg cttcgccaaa
     9121 taaaccattc aaataacgtt caagcgcaac acgcgaacta aagccatgcg ggataccgta
     9181 atgctcatga cgttcacccg ctaaatagag cggaaattgc tggtaatgat cgcaagtttt
     9241 aatatcggaa gcgggttcag ccagtgttga actacgttct tttaatgttg gatgaatcac
     9301 cagggcggta cgtcccattc gcgcttcgcg attaacatag acataatttt ccccacggcg
     9361 gtaaccatac gctttctggg ttaccacatc gacagtaaaa ccgacttttt caagtacgcg
     9421 cgccacctcg tctggtcgta aatacatatt tgatcctcgt tattatcaac cgcgggccta
     9481 ccttacctga ttgcgcattc acaacgcttt cagaaaagtc cataaagccg cgatgaacct
     9541 cagttaagaa aattatggtc tacactgaaa attacatcga attctaatgg gggatcatat
     9601 gtttaaccga ccgaaccgca acgacgttga tgatggcgtg caggatatcc agaatgatgt
     9661 caatcaatta gctgacagtc tcgaatctgt attgaagtcc tggggcagcg acgccaaagg
     9721 ggaagccgaa gccgctcgta gtaaagcgca ggctctactg aaagaaacca gggcgcgaat
     9781 gcatggtcgt actcgcgtcc agcaagccgc gcgcgatgcc gttggctgcg cggattcttt
     9841 tgttcgtgaa agaccctggt gtagtgtggg tacagcggca gcggtgggta tttttatcgg
     9901 cgcactgtta agcatgcgca aatcgtagtg caaaaatgat aataaatacg cgtctttgac
     9961 cccgaagcct ggcttcgggg ttttttagct taaggcttta cgtaattgtt gtgctacctg
    10021 tcgcgccata ccttcgttaa cgatattagt aaacgaaagc agtaatccac cttccccact
    10081 tgaacggata cgccagtcgc taagtgcctg caccgcaagg cctgcagcat tggctttacg
    10141 cgcatgggca atatcatcgc ctatcattct gatcaccatc tggataccac ctttctgcgg
    10201 cacaacctga aatccttgct gcgtcaacgc ttgctcaatc cattgccgac gttgagcata
    10261 atgctgacgc attttcttca gatgccgcca gaaatgcccc tcgcgaagaa agtctgccag
    10321 tgtgttctgc catagaacag gtaccgcaca tggagccagt gacgcctggt ggcggaattg
    10381 tgcaatttgc ttcaccggca ccaccagcca cgcacagcgc aatgcaggaa atagcgcttt
    10441 gctgaatgtt ccggcataaa ttacccgctg cggtgcgtcg agacttttta acgccggtaa
    10501 cggcttaccg tgatagcgaa actcactgtc ataatcatct tcaataatcc atgcctgact
    10561 acgatctgcc cattccagta tctgatgcct gcgcgctaaa gagagcgcca cacccagcgg
    10621 actttggtgt gctggtgtta tcagggcaaa acgcgcatca ggataatttt gtattccgct
    10681 tgtgatatcc agtccattgt catcaaccgg cacaggcaaa atttccacat ctggtctgct
    10741 tttttaggta atttatggct ctctttaaca gtccattata gtgctacatt gcgggcgtta
    10801 ataagaggag aaactgtatg actacattac gtcagcctta ctacgaactt agcccggcag
    10861 tgtataacgc actagtgcag gccaaaacgg cactggaaaa tagcacgctg gataccacgt
    10921 tgatggagtt agtttatttg cgcgtctcgc aaatcaacgg ctgcgcattt tgtctggaga
    10981 tgcacagcaa agcattgcgc aaatccggcg tgccacagca caaactggac gccctggcag
    11041 gttggcgcgt aagccatcat tttgatgaac gcgagcgggc ggcgctggca tgggcagaat
    11101 cggtaaccga aattgccaga acccatgcgg aagacgaggt ttatcagcct ttgcttgagc
    11161 atttcagcgc agcagagatc agcgacttaa cgtttgccat cgggctgatg aattgtttta
    11221 accgtctggc cgtttccatg cggatgtaac ttcttgatgc cgggtattcg cccggcaaaa
    11281 aaacacaaaa tcccctaccc cgttgagcca ttcatcggtg gcgtaatcaa tctaaaattc
    11341 ccatatcttg tgtggttgaa tcttttttca actacatcta gtatctctgt atcaacagag
    11401 agacaacccg acgcgtatca tcgcgccgta tcttcatttt aaacggaaat acgaatcatg
    11461 cgcattacta tttacactcg taacgattgc gttcagtgcc acgccaccaa acgggcgatg
    11521 gaaaaccggg gctttgattt tgaaatgatt aatgtcgatc gcgttcctga agcggcagaa
    11581 gcgctgcgtg ctcagggctt tcgtcagttg ccggttgtga ttgcaggcga tcttagctgg
    11641 tctggtttcc gcccggacat gatcaatcgt ctgcatccag cgccacacgc ggccagtgca
    11701 tgagccagct cgtctacttt tccagcagct ccgaaaatac gcagcgtttt atcgaacgtt
    11761 tagggctgcc cgcggtgcgc atcccgctca atgagcggga acggattcag gtagacgagc
    11821 cttacatcct gatcgtgccc tcttacggcg gcggcggtac ggctggtgcg gtgccacgac
    11881 aggtcattcg ctttttaaac gacgagcata accgggcgtt gctgcgcggc gttattgctt
    11941 cgggtaatcg caactttggt gaggcatatg gccgcgccgg agatgtgatt gcccggaaat
    12001 gcggcgtgcc gtggctgtac cgctttgaac tcatgggtac gcaaagcgat atcgaaaacg
    12061 ttcgtaaagg agtaaccgaa ttttggcaac gacaaccgca gaacgcctga cgcaggaaac
    12121 gatggattat cacgctctga atgcgatgct taacctttac gatagcgcag gtcgcattca
    12181 gttcgataaa gatcgccagg cagtagacgc ctttatgacg acgcatgtgc gtccgaacag
    12241 tgtggccttc agtagccagc agcagcgcct gaactggctg gttaacgaag gttactatga
    12301 tgaaagcgtc cttaatcgct actctcgcga ttttgtcatt acgctgtttg cccacgcgca
    12361 cgccagcggt tttcgtttcc agacattcct cggagcatgg aagttttaca ccagctatac
    12421 gttgaagaca ttcgacggta agcgttatct ggaagatttt gccgatcgcg tcacgatggt
    12481 ggcgctgacg ctggcacaag gcgatgaaac actggcgacg cagctcactg atgagatgct
    12541 gtcaggacgc tttcagccag ccacgccaac attcctcaac tgcggtaagc agcagcgcgg
    12601 cgaactggtt tcctgttttc tgctgcgtat tgaagacaat atggagtcaa ttggccgggc
    12661 agtgaattcc gcactacaac tgtcgaaacg cggcggcggc gtagcatttt tgctgtcgaa
    12721 tctgcgggaa gcgggcgcgc caattaaacg tattgaaaat caatcttctg gcgtgattcc
    12781 ggtgatgaaa atgctggaag acgcattttc ctatgccaac caactcggcg cgcgtcaggg
    12841 ggctggggca gtctatttac atgctcatca tcccgatatt ctgcgttttc tcgatacaaa
    12901 acgggaaaat gccgacgaaa aaatccgcat taaaacactg tcgctgggcg tggtgatccc
    12961 ggatatcact ttccatctgg caaaagagaa tgcgcagatg gcgctgtttt cgccctatga
    13021 cgtagagcga gtttatggca agccgtttgc cgatgtcgcc atcagtgaac actatgacga
    13081 actggttgcc gatgaacgca tccgcaaaaa atacctcaac gcccgtgatt tcttccagcg
    13141 actggcagaa atccagtttg agtctggcta tccgtacatc atgtatgaag acacggttaa
    13201 ccgtgctaac cctatcgccg ggcgcataaa tatgagcaat ctctgctcag aaattttgca
    13261 ggttaacagc gcctcagaat atgacgagaa tctcgactat gctcgtacag gacatgatat
    13321 ttcctgcaat ctagggtcgt tgaatattgc ccacaccatg gattcccccg actttgcccg
    13381 cacggtagag actgccgtac gcggtttaac ggcggtgtca gatatgagtc atatccgcag
    13441 cgtgccgtcc atcgaagccg gaaatgccgc ctcacacgcc atcggtctgg ggcagatgaa
    13501 tttacacggt tatctggcac gagaaggcat cgcctatggg tcgccggaag cactggattt
    13561 caccaatctc tatttctata ccatcacctg gcacgcactg cgtacctcga tgttgctggc
    13621 gcgcgaacgc ggtgaaacct tcgccgggtt caaacagtca cgctatgcca gtggtgaata
    13681 tttcagccaa tatctgcaag gtaactggca gccgaaaacg gcgaaagttg gcgaactgtt
    13741 tgcccgtagc ggtattacgt tacctacccg tgagatgtgg gtgcagctgc gcgacgacgt
    13801 gatgcgctat ggcatataca accagaatct ccaggcggtg ccgccaaccg gttctatctc
    13861 ttatatcaac catgccacgt cgagtattca tccgattgtg gcgaaagtag agatacgcaa
    13921 agagggcaaa acaggacgcg tttactaccc tgccccgttt atgactaacg aaaatctggc
    13981 gctgtatcag gacgcttacg aaatcggcgc agaaaagatc atcgacacct acgcggaagc
    14041 gactcgccat gtcgatcagg ggctgtcgct gacgctgttt ttccccgata ccgccaccac
    14101 tcgcgatatc aacaaagcgc agatttacgc ctggcgcaag ggtatcaaaa cgctctatta
    14161 cattcgcctg cgtcagatgg cgctggaagg cactgaaatt gaaggctgcg tctcctgtgc
    14221 actttaagga atatctatga aactctcacg tatcagcgcc atcaactgga acaagatatc
    14281 tgacgataaa gatctggagg tgtggaatcg cctgaccagc aatttctggc taccagaaaa
    14341 ggtgccactg tcgaacgata ttcctgcatg gcagacatta agtgtcgtag aacaacaact
    14401 aacaatgcgc gtttttactg gcctgacgct gctcgacacg ctgcaaaatg ttatcggcgc
    14461 gccctcgctg atgcccgatg cactcacccc ccatgaagag gcggtattat caaatatcag
    14521 ctttatggaa gcggttcatg cccgctctta cagttcaatt ttctcgacgc tgtgccagac
    14581 caaagatgtc gatgccgcct acgcctggag cgaagaaaac gcgccgttgc agcgaaaagc
    14641 acagattatt cagcaacatt atcgcggtga tgatccgctg aaaaagaaaa tcgccagtgt
    14701 gtttcttgaa tcttttttgt tctattccgg tttctggctg ccgatgtatt ttgccagccg
    14761 cggaaagcta accaataccg cagacctgat ccgtctgatt atccgggatg aagcagtcca
    14821 cggttactac atcggctata aatatcagaa aaaccaggaa aagatctccc aggcacagcg
    14881 tgaagagttg aagagtttcg ccttcgattt gttgctggaa ctctatgaca acgagttgca
    14941 atataccgat gagctgtacg ccgaaacccc gtggtctgac gatgtaaaag cgtttctctg
    15001 ttacaacgcc aataaggcgt tgatgaatct gggctacgaa ccgctatttc ccgcagaaat
    15061 ggcggaagtg aatccggcaa tcctcgccgc gctttcgccg aatgccgatg aaaatcacga
    15121 tttcttttcc ggttcaggct cttcatatgt gatgggaaaa gcggtcgaaa cagaagatga
    15181 agactggaat ttctgagggg gttattttca aaaatatcac tacccgctgc agggaaataa
    15241 ttcccgccaa atagctttat atcacgtaaa taatttgtgg tgatctacac tgatactctg
    15301 ttgcattatt cgcctgaaac cacaatattc aggcgttttt ttgctatctt tgacaaaaaa
    15361 tatcaacttt tctcgatttg ctctcagccc ttatatcatg ggaaattccg gcgatttgct
    15421 cacatcaata ttcatgccac atttgccctc aggggttgcc tcagattctc agtatgttag
    15481 ggtagaaaaa agtgactatt tccattgggt aatgtatcga cataaacaaa taataggaat
    15541 ctttctattg catggcaatt aaattagaaa ttaaaaatct ttataaaata tttggtgagc
    15601 atccacagcg agctttcaaa tatatcgaac aaggactttc aaaagaacaa attctggaaa
    15661 aaactgggct atcgcttggc gtaaaagacg ccagtctggc cattgaagaa ggcgagatat
    15721 ttgtcatcat gggattatcc ggttcgggta aatccacaat ggtacgcctt ctcaatcgcc
    15781 tgattgaacc cacccgcggg caagtgctga ttgatggtgt ggatattgcc aaaatatccg
    15841 acgccgaact ccgtgaggtg cgcagaaaaa agattgcgat ggtcttccag tcctttgcct
    15901 taatgccgca tatgaccgtg ctggacaata ctgcgttcgg tatggaactg gccggaatta
    15961 atgccgaaga acgccgggaa aaggcccttg atgcactgcg tcaggtcggg ctggaaaatt
    16021 atgcccacag ctacccggat gaactctctg gcgggatgcg tcaacgagtg ggattagccc
    16081 gcgcgttagc gattaatccg gatatattat taatggacga agccttctcg gcgctcgatc
    16141 cattaattcg caccgagatg caggatgagc tggtaaaatt acaggcgaaa catcagcgca
    16201 ccattgtctt tatttcccac gatctcgatg aagctatgcg tattggtgac cgaattgcca
    16261 ttatgcaaaa tggtgaagtg gtgcaggttg gtacaccgga tgaaattctt aataatccgg
    16321 cgaatgatta tgtccgaacc ttcttccgtg gcgtcgatat tagccaggtg ttcagtgcga
    16381 aagatattgc ccgtcgtaca ccgaacggct taattcgtaa aacccccggc ttcggcccgc
    16441 gttcggcact gaaattattg caggatgaag atcgcgaata tggctacgtc atcgaacgcg
    16501 gtaataagtt tgtcggcgct gtgtctatcg attcgcttaa agccgcgtta acgcagcagc
    16561 aaggtcttga tgcggcgctg attgatgcgc cgttagcggt cgatgcacaa acgcctctta
    16621 gcgaattgct ctctcatgtc ggacaggcac cctgtgcggt gcccgtggtc gacgaggacc
    16681 aacagtatgt cggcatcatt tcgaaaggaa tgctgctgcg cgctttagat cgtgaggggg
    16741 taaataatgg ctgatcaaaa taatccgtgg gataccacgc cagcggcgga cagtgctgca
    16801 caatccgcag acgcctgggg tacaccggcg actgcaccga ctgacggcgg tggcgctgac
    16861 tggctgacca gtacgcctgc gccaaacgtc gagcatttta atattctcga tccgttccat
    16921 aaaacgctga tcccgctcga cagttgggtc actgaaggga tcgactgggt cgttacccat
    16981 tttcgtcccg tctttcaggg cgtgcgcgtt ccggttgatt acatcctcaa cggtttccag
    17041 caattgctgc tgggtatgcc cgcgccggtg gcgattatcg ttttcgctct catcgcctgg
    17101 cagatttccg gggtcggaat gggcgtggcg acgctggttt cgctgattgc catcggcgca
    17161 atcggtgcct ggtcacaggc aatggttacc ctggcgctgg tgttaacagc cctgctgttc
    17221 tgtatcgtca taggtttgcc gttggggatc tggctggcga gaagtccgcg agcggcgaaa
    17281 attattcgtc cactgcttga tgccatgcag accacgcccg cgtttgttta tctggtgcca
    17341 atcgtcatgc tgtttggtat cggcaacgtg ccaggcgtgg tggtgacgat catctttgcg
    17401 ctgccgccga ttatccgtct gacgattctg ggaattaacc aggttccggc ggatctgatt
    17461 gaagcctcgc gctcattcgg tgccagcccg cgccagatgc tgttcaaagt tcagttaccg
    17521 ctggcgatgc caaccattat ggcgggcgtt aaccagacgc tgatgctggc cctttctatg
    17581 gtggtcatcg cctcgatgat tgccgtcggc gggctgggtc agatggtact tcgcggtatc
    17641 ggtcgtctgg atatggggct tgccaccgtt ggcggcgtcg ggattgtgat cctcgccatt
    17701 atcctcgacc gcctgacgca ggccgttggg cgcgactcac gcagtcgcgg caatcgtcgc
    17761 tggtacacca ctggccctgt cggtctgctg acccgcccat tcattaagta actctgcact
    17821 tgcccggtga cgccgggcat atcaccctgc caaaaaaagg aataacaatg cgacatagcg
    17881 tactttttgc gacagcgttt gccacgctta tctctacaca aacttttgct gccgacctgc
    17941 cgggcaaagg cattactgtt aatccagttc aaagcaccat caccgaagaa accttccaga
    18001 cactgctggt aagtcgtgcg ctggagaaat taggttatac cgtcaataaa cccagcgaag
    18061 tggattacaa cgttggctac acctcgcttg cttccggcga tgcaaccttc accgcagtga
    18121 actggacgcc actgcatgac aacatgtacg aagctgccgg tggcgataag aaattttatc
    18181 gtgaaggagt atttgttaac ggcgcggcac agggttacct gatcgataag aaaaccgccg
    18241 accagtataa aattaccaac atcgcgcaac ttaaagatcc gaagatcgcc aaactgttcg
    18301 ataccaacgg cgacggtaaa gcggatttaa ccggctgtaa cccaggctgg ggctgcgaag
    18361 gtgcgatcaa ccaccagctt gccgcgtatg gactgaccaa taccgtgacg cataatcagg
    18421 ggaactacgc agcgatgatg gctgacacca tcagtcgtta caaagagggt aagccggtgt
    18481 tttactacac ctggacgccg tactgggtga gtaacgaact gaaaccgggg aaagatgtgg
    18541 tctggttgca ggtgccgttc tccgcactgc cgggcgataa aaatgccgat accaaactgc
    18601 cgaatggcgc gaattacggc ttcccggtca gtaccatgca tatcgttgcc aacaaagcct
    18661 gggccgagaa aaacccggca gcagcgaaac tgtttgccat tatgcagtta ccagtggcag
    18721 atattaacgc ccagaacgcc attatgcatg acggcaaagc ctcagaaggc gatattcagg
    18781 gccacgttga tggttggatc aaagcccacc agcagcagtt cgatggctgg gtgaatgaag
    18841 cgctggcagc gcagaagtaa tttttattcg ggcggataag gcgtttttgc ctcatccgcc
    18901 gctctgtaca caatgcctga tgcgacgctg gcgcgtctta tcatacctac aaaccacacc
    18961 gcacatttca acaatctatc tttcatccca tattcatcaa catccgctat tattgatttc
    19021 cagcttaatc atcacctgat gaacaaaaat aatgactaaa cctaatcatg agcttagccc
    19081 ggcgctgatc gtgctgatgt ctatcgccac cggtctggcg gtagccagta actattacgc
    19141 ccagccattg ctcgacacca tcgcgcgtaa cttttccctt tccgccagtt cggcaggctt
    19201 tattgttacc gccgcgcagt tgggctatgc cgcaggtctg ctgtttcttg ttcccctcgg
    19261 tgatatgttt gaacgccgcc gcctgattgt ctcgatgacc ttactggcag caggcggcat
    19321 gttaatcact gccagcagtc agtcgctggc gatgatgatc ctcggtacag cattaaccgg
    19381 tttattttca gtcgtggcac aaattctggt tccactggca gcgacgctgg cttcaccgga
    19441 caaacgcggc aaagtggttg gcaccattat gagcggtctg ctattgggga tcttactcgc
    19501 acggaccgtt gccggattgc tggcgaatct cggcggctgg cgcaccgtct tttgggttgc
    19561 ttcgatgtta atggcactga tggcgctggc attatggcgt ggtctgccac aaatgaaatc
    19621 agaaacccac ctcaactacc cacagttgtt gggttccgtt ttcagcatgt ttatcagcaa
    19681 taagatcctg cgcacccgcg cgttgctggg ctgcctgacc tttgccaact tcagcattct
    19741 ctggacctca atggcctttt tgcttgccgc tccacctttt aactacagcg atggtgtaat
    19801 tggtctgttt gggcttgcgg gagctgccgg agcgttgggc gctcgtccgg cgggcggttt
    19861 tgccgataag ggcaaatcgc accacaccac aactttcggt ctgctgctgc tgctgttact
    19921 ttcatggctg gcaatctggt ttggtcacac ttccgtactg gcgctgatta tcggcatcct
    19981 ggtgctggac ctcaccgtgc agggcgtgca tatcactaac cagacggtaa tttatcgaat
    20041 acaccctgat gcgcgtaatc gcctgaccgc aggttacatg accagctact ttattggcgg
    20101 tgccgctggt tcgctaattt cagcctcagc ctggcaacat ggcagttggg ctggcgtgtg
    20161 tctggctggc gcgacgattg ccctggttaa cttactggtc tggtggcgag gttttcatcg
    20221 tcaggaagcc gcaaattaag caatcagcaa ccgttttcgt ggctttacac ttataagggt
    20281 gttaagaagc ccatcagtct gataaggtta agatattcat tcagtctatt tataatatta
    20341 acaatcgtta agcgtacact ctatggaaag ccctactcca cagcctgctc ctggttcggc
    20401 gaccttcatg gaaggatgca aagacagttt accgattgtt attagttata ttccggtggc
    20461 ctttgcgttc ggtctgaatg cgacccgtct gggattctct cctctcgaaa gcgttttttt
    20521 ctcctgcatc atttatgcag gcgcgagcca gttcgtcatt accgcgatgc tggcagccgg
    20581 gagtagtttg tgggttgctg cactgaccgt catggcaatg gatgttcgcc atgttttgta
    20641 tggcccgtca ctgcgtagcc gtattattca gcgtctgcaa aaatcgaaaa ccgccctgtg
    20701 ggcgtttggc ctgacggatg aggtttttgc cgccgcaacc gcaaaactgg tacgcaataa
    20761 tcgccgctgg agcgaaaact ggatgatcgg cattgccttc agttcatggt catcgtgggt
    20821 atttggtacg gtaatagggg cattctccgg cagcggcttg ctgcaaggtt atcccgccgt
    20881 tgaagcagca ttaggtttta tgcttccggc actctttatg agtttcctgc tcgcctcttt
    20941 ccagcgcaaa caatctcttt gcgttaccgc agcgttagtt ggtgcccttg caggcgtaac
    21001 gctattttct attcccgtcg ccattctggc aggcattgtc tgtggctgcc tcactgcgtt
    21061 aatccaggca ttctggcaag gagcgcccga tgagctatga ggttctgctg cttgggttac
    21121 tggttggcgc ggcgaattat tgcttccgct atctgccgct gcgcctgcgt gtgggtaatg
    21181 cccgcccaac caaacgtggc gcggtaggta ttttgctcga caccattggc atcgcctcga
    21241 tatgcgctct gctggttgtc tctaccgcac cagaagtgat gcacgataca cgccgtttcg
    21301 tgcccacact ggtcggcttc gcggtactgg gtgccagttt ctataaaaca cgcagcatta
    21361 tcatcccaac actgcttagt gcgctggcct atgggctagc ctggaaagtg atggcgatta
    21421 tataacccac aagaatcatt tttctaaaac aatacattta ctttatttgt cactgtcgtt
    21481 actatatcgg ctgaaattaa tgaggtcata cccaaatgga tagttcgttt acgcccattg
    21541 aacaaatgct aaaatttcgc gccagccgcc acgaagattt tccttatcag gagatccttc
    21601 tgacccgtct ttgcatgcat atgcaaagca agctgctgga aaaccgcaat aaaatgctga
    21661 aggctcaggg gattaacgag acgttgttta tggcgttgat tacgctggag tctcaggaaa
    21721 accacagtat tcagccttct gaattgagtt gtgctcttgg atcatcccgt accaacgcga
    21781 cgcgtattgc cgatgaactg gaaaaacgcg gttggatcga acgtcgtgaa agcgataacg
    21841 atcgccgctg cctgcatctg caattaacgg aaaaaggtca cgagtttttg cgcgaggttc
    21901 taccgccgca gcataactgc ctgcatcaac tctggtccgc gctcagcaca acagaaaaag
    21961 accagctcga gcaaatcacc cgcaaattgc tctcccgtct cgaccagatg gaacaggacg
    22021 gtgtggttct cgaagcgatg agctaacgcg tcatctcgct caaaaatcca gatttataaa
    22081 agaaaaaata accggccagc atcgtaacat gctggccttt ttggcaagca ggtcggctca
    22141 gccgatgagt taagaagatc gtggagaaca atatgagcgc aaatgcggag actcaaaccc
    22201 cgcagcaacc ggtaaagaag agcggcaaac gtaagcgtct gctcctcctt ctcaccttgc
    22261 tctttataat tattgccgta gcgataggga tttattggtt tttggtactg cgtcatttcg
    22321 aagaaaccga tgacgcatac gtggcaggga atcagattca aattatgtct caggtgtctg
    22381 gcagcgtgac gaaagtctgg gccgataaca ccgattttgt aaaagaaggc gacgtgctgg
    22441 tcactctcga cccgacagat gctcgccagg catttgaaaa agccaaaact gcactggctt
    22501 ccagcgttcg ccaaacccac cagctgatga ttaacagcaa acagttgcag gcgaatattg
    22561 aggtgcaaaa aatcgccctc gcgaaagcac aaagcgacta caaccgccgt gtgccgctgg
    22621 gcaatgccaa cctgattggt cgcgaagagc tgcaacacac ccgcgacgcc gtcaccagtg
    22681 cccaggcgca actggacgtc gctattcaac aatacaatgc caatcaggcg atgattctgg
    22741 ggacgaaact ggaagatcag cctgccgtac aacaggctgc caccgaagta cgtaacgcct
    22801 ggctggcgct ggagcgtacc cgtattgtca gtccgatgac cggttatgtc tcccgccgcg
    22861 cggtacagcc tggggcgcaa attagcccaa cgacgccgct gatggcggtc gttccagcca
    22921 ccaatatgtg ggtggatgcc aactttaaag agacgcagat tgccaatatg cgtatcggtc
    22981 agccggtcac catcaccacc gatatttacg gcgatgatgt gaaatacacc ggtaaagtgg
    23041 ttggtctgga tatgggcaca ggtagcgcgt tctcactgct tccggcgcaa aacgccaccg
    23101 gtaactggat caaagtcgtt cagcgtctgc ctgtgcgtat tgaactggac cagaaacagc
    23161 tggagcaata cccgctgcgt atcggtttat ccacgctggt gagtgtcaat accactaacc
    23221 gtgacggtca ggtactggca aataaagtac gttccacacc ggtcgcggta agcaccgcgc
    23281 gtgaaatcag cctggcacct gtcaataaac tgatcgacga tatcgtaaaa gctaacgctg
    23341 gctaatccag aggtgcgtgt gatgcaacag caaaaaccgc tggaaggcgc acaactggtc
    23401 attatgacga ttgcgctgtc actggcgaca ttcatgcagg tgctggactc caccattgct
    23461 aacgtggcga tccccactat cgccgggaat ctgggctcat cactcagtca gggaacgtgg
    23521 gtgatcactt ctttcggggt ggcgaatgcc atctcgatcc cgcttaccgg ctggctggca
    23581 aagcgcgtcg gggaagtgaa attgttcctc tggtcaacca tcgcctttgc tattgcgtcg
    23641 tgggcgtgtg gtgtctccag cagcctgaat atgctgatct tcttccgcgt gattcagggg
    23701 attgtcgccg ggccgctgat cccgctttca caaagtctat tgctgaataa ctacccgcca
    23761 gccaaacgct cgatcgcgct ggcgttgtgg tcgatgacgg tgattgttgc gccaatttgc
    23821 ggcccgatcc tcggcggtta tatcagtgat aattaccact ggggctggat attcttcatc
    23881 aacgtgccga ttggcgtggc ggtggtgttg atgacactgc aaactctgcg cggacgtgaa
    23941 acccgcaccg aacggcggcg gattgatgcc gtggggctgg cactgctggt tattggtatc
    24001 ggcagcctgc agattatgct cgaccgcggt aaagagctgg actggttctc atcacaggaa
    24061 attatcatcc tcaccgtggt ggcggtggtg gctatctgct tcctgattgt ctgggagctg
    24121 accgacgata acccgatagt cgatctgtcg ttgtttaagt cgcgcaactt caccatcggc
    24181 tgcttgtgta tcagcctcgc gtatatgctc tacttcggcg cgattgttct gctgccgcag
    24241 ttgttgcagg aggtctacgg ttacacggcg acgtgggcag ggctggcctc tgcgccggta
    24301 gggattattc cggtgatcct gtcgccgatt atcggccgct ttgcgcataa actggatatg
    24361 cgccgactgg taaccttcag ctttattatg tatgccgtct gcttctactg gcgtgcctat
    24421 acctttgaac cgggtatgga ttttggcgcg tcggcctggc cacagtttat ccagggtttt
    24481 gcggtggcct gcttctttat gccgctgacc accattacgc tgtccggttt gccaccggaa
    24541 cgactggcgg cggcatcgag cctctccaac tttacgcgaa cgctggcggg gtctatcggc
    24601 acgtcgataa ccacgaccat gtggaccaac cgcgagtcac tgcaccatgc acagttgact
    24661 gagtcggtaa acccgttcaa cccgaatgct caggcgatgt acagtcaact ggaagggctt
    24721 ggtatgacgc aacagcaggc atcaggctgg attgcccagc agataaccaa tcaggggctg
    24781 attatttccg ccaatgagat cttctggatg tcagccggga tattcctcgt cctgctgggg
    24841 ctggtgtggt ttgctaaacc gccatttggc gcaggtggcg gcggaggtgg tgcgcactaa
    24901 ggttgccaaa gggagctgcg gctccctttt tatttgctca ttctgcaaac ggttgtttta
    24961 tacactcatt cggtgtatct tttcgccgcc ccaaactctt ccattcacca ttaaccggtt
    25021 ctgagcattc gctcaggttg ttaattctct ttttccttcg cccttgcttt cgcgggtgat
    25081 actcacattt ttattttaac cccataactc gcaccctcgc gggcgtacat ttattacaca
    25141 caaaataatg gattattatg aatacccaac gaaaatacgg gagaacctgg cattacccct
    25201 tctctccagg caccaccagc gatgaccgca ttaacaccga ttactggcag gatttaaagg
    25261 ccatcacgca attggtgcac acagaaaaac tcgatggcga aaataactgc cttaaccgct
    25321 acggcgtgtt tgcccgctcc catgccacgc ctacgcagtc ggcatggacc tataaaattc
    25381 gccaacgctg gcaactgctg aaaaacgatc tcggcgacct ggagttgttt ggtgaaaatc
    25441 tctacgccgt tcattccatt gaataccgcg cgctggaaca ggacttttac ctgtttgccg
    25501 ttcgctgcca ggatatgtgg ctgagctggg aagaggtgca gttttacgcc gctctgtttg
    25561 atttcccctg cgttcccgaa atctccggcc cgcagccggg gaatgatgaa aaaagctggc
    25621 aacgtgattt tctggcgctg accaatgcgc gcggcacatt cgatccctgg gatacgcaaa
    25681 cctgtcaacc ctgtacgctg gaaggcattg tcagccgaaa tcatgatgcg ttttctgtcg
    25741 ctgacttttc gcataacgtc ttcaagtatg tgcgcaaaaa tcatgtcaaa accactgtgc
    25801 actggaaacg ccactggcaa cgggcgcgga tggcccatga gttcgtttat ggagaacagt
    25861 catgatttgg caacttaccg acgataaacg ctggtcggcg ctacgtcagc gttttagctg
    25921 ggttgaggag atgcatcaca cgccacaaga ccctgagcat catggtgaag gcgacgtcgg
    25981 cgtacatacc gaaatggtgc ttaacgccct cattactctg ccagaattcc agcaactccc
    26041 cgctcaacag caggaagttt tatgggcggc agcgttattg catgatgttg aaaagcgcag
    26101 cactaccgtg caggaaaacg gacgtattca gtctcctggt catgctcgtc ggggcgaact
    26161 gacggcccga caaattttat ggcgcgatat cccaacgccg ttcgtgctgc gcgagcaaat
    26221 tgttgcactg gtgcgtctgc ataggttacc gctatggtta ctggagcgcc cggaaccgga
    26281 gcgtttgttg ctcgcggctg caatgcgcat cgacacgcgt ctgctcgctt tgcttgctcg
    26341 tgccgatctg cttggtcgcc aaagcccgga tcagcaatca atgctggaac gcatcgatct
    26401 gttcgagctg ttttgccatg agcaacaatg ctggggaaaa atgcgcccgt tcgtttctga
    26461 ttccgcacgc tggcattacc tgacccatga gcaaagttct cctgattttg ttccctggga
    26521 ggctgaacct tttgaggtta ttttgctgtg tggcttaccg ggtatgggca aagatcgcta
    26581 tatcaatgaa cagtgccagg gaatggacgt tatcagcctt gatgatatgc ggcggcgaat
    26641 caatgccagt ccggacgaca aaaccgccac cgggcgtatt gtgcaacagg caaaagaaga
    26701 ggcacgcgtt tttttacgcc agaaaaagcc gttcatctgg aatgcaacga acattacccg
    26761 ccagctgcgt agccagctca tcagcctgtt tacggcttac ggcgcacgag taaaaatcgt
    26821 ttatcttgaa gtgccatggg cgcaatggaa gcagcaaaat gccagacgcg agtatgcagt
    26881 tcctgaagcg gtcgtgatgc ggatggcctc aaggctggaa gttcctcaac ctgatgaagc
    26941 acatagcgtg gaatatcgga tgactgacag gtaacaaaag ccgggcggtg ttttcaccag
    27001 cccggcctct tctgcttaaa ctgactaaat gtgcagttcc tgcaacttct ctttcggcag
    27061 cgccagttct tcgttgctgt tgatgcgcac gtcacgttcc agaatgttac gcgcaatatc
    27121 ctgcgcttcc tgcaacgagt gcatctggta agtgccacac tggtagacgt tcagttccgg
    27181 gatctgattc tgatcctgca ctttcagcac gtcttccatt gccgctttcc aggcatcagc
    27241 gacacgctgc tcatctggcg taccaatcag actcatataa aaaccggtgc ggcagcccat
    27301 tggcgagata tcgataatct ctacgccatt accgttaaga tggttacgca taaaaccagc
    27361 aaacaggtgc tccagggtat ggatccctct ttctggcatc acttctttgt tcggcacgca
    27421 gaagcgcaga tcgaacacgg tgattgcgtc gccatgcggg gtgttcattg tttttgccac
    27481 ccgaactgca ggcgcttcca tccgagtatg atcgactgtg aagctatcta acaacggcat
    27541 ttagccacct ccggtaattt ttttaaaaat tttctgaact ctttgttccc aggcgagtct
    27601 gagtatatga aagacgcgca tttgttatca tcatccctga attcagagat gaaattttgg
    27661 ccactcacga gtggcctttt tcttttctgt caggcgtgct tttccagcca caccgcaaac
    27721 ggttcggtat cagcggcttc catttcctgc tgacggcgtt cagacgcctc gcgctcggct
    27781 acaaaatcct cttcgcgcag aatttccagc ggctcttcac gcagcagatt acggtacgct
    27841 tcagcaaacg ctttgcctgt tccgccaata ccagtatcaa tcatagacct taagatacgg
    27901 gcagagaaag tcagatcagg attatcgaag caggcgacca gttcatcaca cactttctga
    27961 tacgcttcgc cgccgttaat gctatccagc gtttgcgcga cgcgtttcag atcgcggaac
    28021 agatctttac ccacctgcgg taacgggaac tgcgcagttt cgcagccgat acccagcgtc
    28081 aggcccggtt tgcgaccttc gaggatcacc cggttccagt taacgcgtgt acaggcaagt
    28141 tcgctactgc tcatttccgg tgcgtcagcc agcgcacacc agaccataaa caggtcgagg
    28201 aatcgcacct gttgttcatc tacaccaatc ggcgagaacg ggttgatgtc cagcgagcgg
    28261 acttcaatat attcaatgcc gccacgtaac agcgcatcag aaggcgactc gccgctgcgg
    28321 gtaacgcgtt ttggacgaat cggcgcgtac agttcgtttt caatctgcag cacgttgctg
    28381 ttgatttgca gtcgcttacc gtctttctca ataccaatct tcgcgtactc ttccgatggc
    28441 gttttgattg cctgtttaag gcccgccacg tactcgtaga gatcattgaa ggtgatacca
    28501 agattgcttt gcgatttatt ggtatagccg agatcgctca aacgaagaga ggtcgcatac
    28561 ggcaggtaat acataccgca ctcggttttc tcaaacggca gcgacgttgg ttttccttgc
    28621 aagaaagaag aacaaatcgc tggagacgca ccaaacagat aaggaatgac ccaaccgaag
    28681 cgatagtagt tacggataac acggaaataa cccgcagata ttttctcttt ggcatcagcg
    28741 cccgagatat caccgcactt cgcttgccag aatgccattg gcaaagagaa attatagtgc
    28801 acgccggaaa tggtttgcat cagcgcgccg tagcgatttt tcagcccttc acggtacagc
    28861 gttttaaagc gaccggtgtt agaagtgccg tactgtgcca gttcgatgtc ctgaccttct
    28921 gcgatatagc atggcatact taacggccac atccgctcat cgcccatatt gcgcgccgta
    28981 tagcgatgca gatcgcgcat aaaggtcagc atatgttcaa tatcaccatc cactggtgta
    29041 ataaattcca gcaatgcttc cgcaaaatcg gtagtaatcc atttatgggt cagtgcagaa
    29101 cctaatgctt caggatgacc tgttgttgcc agtgtgccat cagcattaac acgcaaagtt
    29161 tcgcgctcca gcccacgctg tatccccttt aacgcctgag gatgtttttc cagccaggcc
    29221 agcgcctgtg atacgtccgg gatcaaattg acctcccgcc tgtcaaaatc gttttaatta
    29281 gcataactgt aatggtgacc atatgtgcag gcctacaatt agtgccacca catcatgccc
    29341 tgaacggtag ctgctgcaac tgcaacatag cgtaacgctt taccaaggca caaaaaaaag
    29401 attaccggtc cccacgagat gcgcatccat cccgctaaca gacacagtaa atcgccaacc
    29461 acgggcatcc agcttaataa tagcgtgact gcaccatagc gtttcagcca gccggtagct
    29521 ttctcttgcc agcgcgatgt tttacgcaat ggaaagaaac gcccaaggat aacgttagtt
    29581 aaccctccaa ggctattacc cattgttgct gttaaaacta aaacccaggg atgactcact
    29641 ccggaaagca acattgccac cagcacgact tcggagttgc cgggtaatag tgtagcgctg
    29701 agaaaactac tggcaaacaa cgagaaaagc gataacgctt cactcacagc aagcgaacat
    29761 ccacggcgtc catgcctgct gcacgggccg cctgaatacc gaaatcggca tcttcaaaga
    29821 ccacacactg cgtcggttgc acgcccatac gctgcgcgca caacaaaaat gtgtctggcg
    29881 cgggtttatg gtgtttgacg tgatcggcag cgacgacggc gtcaaaataa cggcgtaatc
    29941 ccaggtgtgc cagcaatgcc tcagcgatgg cgctttcact ccccgttcct acagccattg
    30001 ggcgacgacc atgccagctt ttcaccactt caacaagagg aagcggctcg acactatcca
    30061 gcagcatact tcttactgct tctgtttttt cacgcgctaa cgcatgcggg tcgagatcgg
    30121 cctgattcag ctcaataatc gcctgagcaa tacgccaggt gggcgagcca ttaagcgcaa
    30181 tcatcgcctg aacatcgtac tgaagaccgt agtaccctaa tacttcgcgc cacgctttac
    30241 ggtgcgtagg ctcggtatcc aggatggtgc catccatatc aaaaattaaa cctgcataac
    30301 gctcgtacat ggtcttctcg caagtagaaa aatatgacgc tactttagcg taattgcttg
    30361 attttgtcgc tgatggagaa ggagaagaaa attcaggaaa tggataaagt agttatttag
    30421 aaatgagata tttttgaagg aaattttttg gagaatatgg tgcatccggg aggatgactc
    30481 actggcgttc gcccttcggg ccgctgctgg cgcaacgtta tcctcccagg tgcctgcggt
    30541 taataccgct acctcttcga taccttcatt gctgaaggtt acggagaata tggtgcatcc
    30601 gggaggattc gaacctccga ccgctcggtt cgtagccgag tactctatcc agctgagcta
    30661 cggatgcatc ggaaaaattt attttactgc tgatattgat atcgc
//
LOCUS       DAZFFE010000047        30174 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFFE010000047 DAZFFE010000000
VERSION     DAZFFE010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.047"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            302..1609
                     /gene="csy1"
                     /locus_tag="ACHJ69_002363"
     CDS             302..1609
                     /gene="csy1"
                     /locus_tag="ACHJ69_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy1"
                     /protein_id="HFO5015336.1"
                     /translation="MEKTALIQFITDYIASRRQPKIDAFEKEAAKRLEQGEDASVIAL
                     ERQELEARYLPRNWLTDAAKRAGQIKLVTHGAKFSHGDSKASSFYLETSATESYLNTA
                     SLANVATDAIGNAAALDVAKLLQTDVNGDSLLASLKRGDYQALSTFAEDKVQLELWVA
                     GFSQAWTTGRPSSHKLAKQIYFPVADGYHLLCPLFSSSLAQAMYEKLTAGRFSEESKA
                     IRDARKAGKWHSQPDIRFPNLAEMHFGGTKPQNISLLNSVRGGRVWLLPSMPPAWGPL
                     DRAPQKMHSIFALRGDFNRAASGIIARMTYLLKVDTNNVHIRTVRAKYIDELIDLLFM
                     QASAFQQEKWQGWSAQSPDLPRHQQLWLDPWRSLSDETFKQEREKNDWQVTVADDFAR
                     WLNYRLKKSSFDVGAVEQKEWRSQSLFAQRMREMEAVLQEALK"
     gene            1606..2529
                     /gene="csy2"
                     /locus_tag="ACHJ69_002364"
     CDS             1606..2529
                     /gene="csy2"
                     /locus_tag="ACHJ69_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy2"
                     /protein_id="HFO5015337.1"
                     /translation="MSYLLLLPHIRIENANAVSGLTWGFPSMTHFLGYVHALSRKVVD
                     EFGVSFDGCAVVSHEQHIQAYSSGRDFQFALTRNPLTREGKTASFNEEGRMHLTVSLL
                     IECNGEITNGEYGRKVLCDYLKMLCQSHKLAGGSIVSMRDPQLFHAPEDEKQLRKIVW
                     RLMPGYALYDRSEWLAEHHQQHPDISLLDAWLDFAAIKYQAESPAEDNSAKWVYQPKP
                     MPGFLVPLMCGYQRISPVYAPGEVENARDTVTPFAFAEAVYGIGEWRGLHRTTDLQAL
                     MWRYRTTDTGYYCSATPVVDDFTFNEYDDLE"
     gene            2540..3541
                     /gene="csy3"
                     /locus_tag="ACHJ69_002365"
     CDS             2540..3541
                     /gene="csy3"
                     /locus_tag="ACHJ69_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy3"
                     /protein_id="HFO5015338.1"
                     /translation="MAKAPVAIKTASVLAFERKLATSDAVMYAGNWQGNEWQPIEIQE
                     KAVRGTISNRLKNAIVSDPTKLDAEIQKANLQRVDVASLPVDTDTLKVVFTLRVLGNL
                     STPSVCNDMAYQEALSGVIEGYISEHGFKELTLRYALNLANGRFLWRNRIGAEQIQVK
                     VTANETSWTFNSHDFSLRQFDQGQAAVTQLAAIIEQGLSAKEWVMLTVEAQVRLGAGQ
                     EVFPSQELVLDSNSSKSRVLYQVAGIAGIHSQKIGNALRTIDTWHPKVDELGAIAVEP
                     YGSVTSRGVACRQPKEKLDFYTLLDNWVTKGMKPDVKQQHYVMAVLIRGGVFGEKSE"
     gene            3552..4106
                     /gene="cas6f"
                     /locus_tag="ACHJ69_002366"
     CDS             3552..4106
                     /gene="cas6f"
                     /locus_tag="ACHJ69_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated endoribonuclease
                     Cas6/Csy4"
                     /protein_id="HFO5015339.1"
                     /translation="MDHYLEIRVLPDPEFSSEMLMAALFAKLHRVLGARGQGDIGVSF
                     PDVNVMPGTHLRLHGSAQALQELEASTWRKGLTDYCQCSPVTPVPEIKGWRVVSRVQV
                     KSNPQRLLRRSVKKGWLTEEQAIERLATQAEQRTDLPFLNMKSLSSQQQFKLFIRHGD
                     LLKEPVKGEFSSYGLSATATIPWF"
     gene            complement(4218..4305)
                     /locus_tag="ACHJ69_002367"
     tRNA            complement(4218..4305)
                     /locus_tag="ACHJ69_002367"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4269..4271),aa:Ser,seq:gga)
     repeat_region   4649..4859
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=4649..4679
                     /rpt_unit_seq="gttcactgccgtacaggcagcttagaaattt"
     gene            complement(4969..5187)
                     /gene="infA"
                     /locus_tag="ACHJ69_002368"
     CDS             complement(4969..5187)
                     /gene="infA"
                     /locus_tag="ACHJ69_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO5015340.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(5472..6176)
                     /gene="aat"
                     /locus_tag="ACHJ69_002369"
     CDS             complement(5472..6176)
                     /gene="aat"
                     /locus_tag="ACHJ69_002369"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO5015341.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCDEFIRHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(6218..7939)
                     /gene="cydC"
                     /locus_tag="ACHJ69_002370"
     CDS             complement(6218..7939)
                     /gene="cydC"
                     /locus_tag="ACHJ69_002370"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO5015342.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DLVSLTLRDVQFTYPEQSQQALKGIYLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMCEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(7940..9706)
                     /gene="cydD"
                     /locus_tag="ACHJ69_002371"
     CDS             complement(7940..9706)
                     /gene="cydD"
                     /locus_tag="ACHJ69_002371"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO5015343.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLFLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQTALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(9829..10794)
                     /gene="trxB"
                     /locus_tag="ACHJ69_002372"
     CDS             complement(9829..10794)
                     /gene="trxB"
                     /locus_tag="ACHJ69_002372"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO5015344.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            11338..11832
                     /gene="lrp"
                     /locus_tag="ACHJ69_002373"
     CDS             11338..11832
                     /gene="lrp"
                     /locus_tag="ACHJ69_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO5015345.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            11967..15956
                     /gene="ftsK"
                     /locus_tag="ACHJ69_002374"
     CDS             11967..15956
                     /gene="ftsK"
                     /locus_tag="ACHJ69_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO5015346.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPTQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPTRPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREVQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNTEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQYQQPQQPVAQQPQQPV
                     AQQPQYQQPQQPVAPQPHDTLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPV
                     DTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLA
                     RSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDI
                     AGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELS
                     VYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRM
                     MRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKAR
                     AAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDM
                     LYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGGVGGFDGA
                     EELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNR
                     EVLAPPPFD"
     gene            16115..16726
                     /gene="lolA"
                     /locus_tag="ACHJ69_002375"
     CDS             16115..16726
                     /gene="lolA"
                     /locus_tag="ACHJ69_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO5015347.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            16737..18080
                     /gene="rarA"
                     /locus_tag="ACHJ69_002376"
                     /gene_synonym="mgsA"
     CDS             16737..18080
                     /gene="rarA"
                     /locus_tag="ACHJ69_002376"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO5015348.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            18171..19463
                     /gene="serS"
                     /locus_tag="ACHJ69_002377"
     CDS             18171..19463
                     /gene="serS"
                     /locus_tag="ACHJ69_002377"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO5015349.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            19702..22146
                     /gene="dmsA"
                     /locus_tag="ACHJ69_002378"
     CDS             19702..22146
                     /gene="dmsA"
                     /locus_tag="ACHJ69_002378"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO5015350.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATKMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQEFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGISNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            22157..22774
                     /gene="dmsB"
                     /locus_tag="ACHJ69_002379"
     CDS             22157..22774
                     /gene="dmsB"
                     /locus_tag="ACHJ69_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO5015351.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            22776..23639
                     /gene="dmsC"
                     /locus_tag="ACHJ69_002380"
     CDS             22776..23639
                     /gene="dmsC"
                     /locus_tag="ACHJ69_002380"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO5015352.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMLGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELANIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPEVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(23675..24301)
                     /gene="ycaC"
                     /locus_tag="ACHJ69_002381"
     CDS             complement(23675..24301)
                     /gene="ycaC"
                     /locus_tag="ACHJ69_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO5015353.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNENFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            24615..25763
                     /gene="ycaD"
                     /locus_tag="ACHJ69_002382"
     CDS             24615..25763
                     /gene="ycaD"
                     /locus_tag="ACHJ69_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5015354.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEQMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTALTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            complement(25860..26600)
                     /gene="pflA"
                     /locus_tag="ACHJ69_002383"
     CDS             complement(25860..26600)
                     /gene="pflA"
                     /locus_tag="ACHJ69_002383"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO5015355.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMSEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(26792..29074)
                     /gene="pflB"
                     /locus_tag="ACHJ69_002384"
     CDS             complement(26792..29074)
                     /gene="pflB"
                     /locus_tag="ACHJ69_002384"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO5015356.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(29129..29986)
                     /gene="focA"
                     /locus_tag="ACHJ69_002385"
     CDS             complement(29129..29986)
                     /gene="focA"
                     /locus_tag="ACHJ69_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO5015357.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         7535 a         7389 c         7918 g         7332 t
ORIGIN      
        1 tacaagtccg tatccggaga tatctctatt tctaagctgc tcaaggcagt ttgggtatgt
       61 tagcgcttca actattgcgt ggcaaatatt aagttgcttt aatatcaccg atggtgatta
      121 catgagattt aataatatct tttagaagat atctctagga gagtttgttt gtgtgatcac
      181 cactggtgtt gtcccttttg ctgtgtatgt cagttgtact gaagccatgt tgggctacgg
      241 agatttttca gtgaaatcag ggagcaactt cgacttgatg gattaacgaa aaggagccga
      301 tatggaaaaa acggcgctaa tacaatttat tactgactac atcgcttcgc gaaggcaacc
      361 caaaatagat gcctttgaaa aagaagcagc aaagcggttg gaacagggcg aggacgccag
      421 cgttattgct ctggagcggc aggaacttga agcacgctac ctgccacgta actggttaac
      481 ggatgcggcg aaacgtgccg ggcaaatcaa attagtcacc catggcgcaa aattcagtca
      541 tggtgattct aaggccagca gcttttatct ggaaacctcc gctaccgaaa gctatttaaa
      601 cacagcatcg ttggcgaatg tcgctactga cgcgattggc aacgccgccg cactggacgt
      661 cgcaaaactg ctacaaaccg atgtgaacgg agattcactg cttgccagcc tgaagcgtgg
      721 tgattatcag gcattaagta cgtttgcaga ggataaagtg caactggagc tgtgggtagc
      781 tgggtttagc caggcatgga caactggacg gccttccagc cataaactgg cgaaacaaat
      841 ctatttccct gttgcggatg gttatcacct gttatgccca ctgttttcca gttcactggc
      901 ccaggcgatg tatgaaaagc taaccgccgg gcgctttagt gaggaatcga aagccattcg
      961 cgatgcccgc aaagcaggaa aatggcactc ccagccagat atccggtttc ctaatcttgc
     1021 cgaaatgcat ttcggcggca caaaaccgca gaatatttct ttgctaaaca gtgtgcgtgg
     1081 tgggcgcgtc tggctgttgc cgtcaatgcc gcctgcctgg gggccgctgg atcgcgcacc
     1141 acaaaaaatg cacagtattt ttgccttacg cggcgatttc aatagggcgg cttcaggcat
     1201 catagcccgg atgacttacc tgctgaaggt tgacacaaat aatgttcata tccgaactgt
     1261 gcgggcgaaa tatattgatg agttgatcga tttgctgttc atgcaggcat ccgcttttca
     1321 gcaagaaaaa tggcagggat ggtccgcaca atcaccagac ttacctcgcc accaacaact
     1381 ttggctggac ccgtggcgaa gcctctcaga tgagactttc aaacaggagc gtgagaaaaa
     1441 cgactggcaa gtaactgtcg ccgatgattt cgcacgctgg ctgaactatc gcctgaaaaa
     1501 atccagtttt gacgtaggcg ctgtcgaaca aaaagagtgg cgcagccagt ctctgttcgc
     1561 tcaacggatg cgtgaaatgg aagctgtttt gcaggaggcg ctgaaatgag ttatctgctc
     1621 ttgttacccc atatacgcat cgaaaacgct aacgctgtct ccgggttgac ctggggattc
     1681 ccgtcgatga cccattttct cggttatgtc catgcgcttt ctcgcaaagt cgtggatgaa
     1741 tttggcgtaa gttttgatgg ttgtgcggta gtaagccatg aacagcatat tcaggcgtac
     1801 agctctgggc gcgattttca gttcgctctc acccgtaacc cattaaccag agaaggtaaa
     1861 accgcctcgt tcaatgaaga gggccgcatg cacttgacgg tttctctact gattgaatgt
     1921 aatggcgaaa tcaccaatgg tgaatatggc agaaaagttc tttgtgacta tctgaaaatg
     1981 ctttgccaga gccacaaact ggcgggcggc agcattgtca gtatgcgcga tccgcagctt
     2041 tttcatgcgc ctgaagatga aaagcaactg cgtaaaatcg tctggcggtt aatgccgggt
     2101 tatgcgcttt acgatcgcag tgaatggctg gctgaacatc accaacaaca tcccgacatc
     2161 tcgttgctgg atgcctggct tgatttcgcc gcgattaaat atcaggccga atctccggct
     2221 gaagacaaca gcgcaaaatg ggtataccag ccgaagccaa tgcctggttt cctggtaccg
     2281 ttaatgtgtg gctaccaacg catttctcct gtttacgcac ccggtgaagt tgaaaatgcg
     2341 cgcgacaccg tgacgccatt tgcctttgcc gaagcggtat atggcattgg cgaatggcgc
     2401 ggattacatc gcactacaga cttgcaagcg ctgatgtggc gttaccgcac aacggacact
     2461 ggctactatt gttcggcaac gccagttgtt gatgatttta cctttaatga atatgacgat
     2521 ttagaataag gacataatta tggcaaaggc tcctgtagca ataaaaacgg cgtcggtact
     2581 ggcatttgag cgcaaactgg caacctctga cgcggtaatg tacgcaggta actggcaagg
     2641 aaatgaatgg caacccattg agattcagga gaaagcggtc cgcggaacca tctctaatcg
     2701 cctgaaaaat gccattgtca gcgatcccac aaaactggat gctgaaatcc aaaaagccaa
     2761 cctgcaacgg gtggatgtgg cttcgcttcc ggtagatacg gatacgttaa aggtggtgtt
     2821 tacgctgcgt gtgctgggta atttatccac accgtcagta tgtaatgata tggcttacca
     2881 ggaggcgctt tccggggtca ttgaaggtta catcagcgaa catggcttta aagagctgac
     2941 gcttcgctat gcgctgaatc tggcaaatgg tcgttttctg tggcgcaacc gcattggtgc
     3001 cgaacagatc caggtgaaag tgactgctaa cgagacttca tggactttta acagccatga
     3061 cttctcttta cgtcaatttg accagggaca ggcagcggtc acgcagctgg cggccattat
     3121 agagcaagga ttaagcgcaa aagagtgggt gatgcttacc gttgaagcgc aggttcgtct
     3181 tggtgccggg caagaagttt ttccgtcgca ggagctggta ctcgacagca acagtagtaa
     3241 aagtcgcgtg ttataccagg tggcaggcat tgccggtatt cactctcaga agattggcaa
     3301 tgctttacgt accattgata cctggcatcc aaaggttgat gaattggggg ctatagccgt
     3361 ggaaccttac ggttctgtaa ccagtcgcgg tgtggcctgc cgtcaaccga aagaaaaact
     3421 cgacttttat accttactgg acaattgggt gactaaaggg atgaaaccag acgttaagca
     3481 gcagcactac gtgatggctg tgctgatccg tggcggtgtc tttggtgaga aatcggaata
     3541 aggagctgcc aatggatcac taccttgaga tccgcgttct gccagatcct gaattttcga
     3601 gcgaaatgtt gatggcggcg ttatttgcaa aactgcatcg ggtattaggt gcaagagggc
     3661 aaggcgatat cggcgtgagt ttcccggacg taaatgttat gcctggaacg catttacgct
     3721 tgcatggtag cgcccaggca ttgcaagaac ttgaagcctc gacgtggcgt aaagggctga
     3781 cagattactg ccaatgttcg cctgtcacgc cagttcctga aattaaaggc tggcgagtcg
     3841 taagtcgtgt acaggtaaaa agtaaccctc agcgtctgtt aaggcgttca gtgaaaaaag
     3901 gctggctaac tgaagaacag gcgattgaac gactggcgac gcaggctgaa cagcggaccg
     3961 atttaccctt tcttaatatg aagagtttgt cgagtcagca acagttcaag ttgtttatcc
     4021 gtcatggtga tttgctcaaa gaaccggtta aaggggaatt tagtagttat ggattaagcg
     4081 caaccgccac tattccctgg ttttgatgct ttgcaatttt taacaggcct ggccatgtgg
     4141 ttgggccttt ttatgcgcgc catgcccacc gcatacaaaa aaaagcccgt actttcgtac
     4201 gagctcttct ttaaagatgg cggtgaggcg gggattcgaa ccccggatac gttgccgtat
     4261 acacactttc caggcgtgct ccttcagcca ctcggacacc tcaccaaatt gttttgctgc
     4321 cagacctcat aggtggcaac ggggcgctac tatagggagt tggagtaaaa cggtcaagaa
     4381 gaatttatat agattgattc gtttggttac gcaatgaaca cactgttggt gggacggaga
     4441 ttatgactgt atgtgttctg gtcaattgtt tatcaaaagc tatgcagaaa atatgagatt
     4501 gaagaaatac caaatcgtcc ctttttttag ggcgaaatgt aactcattga ttttgtttac
     4561 tgctattttg aagtctggaa aaagggggtg aatctgcgat tttgtaagtt ttaacagtaa
     4621 atcaatcgga tagtctgcta ttattccagt tcactgccgt acaggcagct tagaaatttt
     4681 aatttttcca gataattttg gcgagtctgt tcactgccgt acaggcagct tagaaatttc
     4741 ccggcaggca tggcaagtta cgtctcccgt tcactgccgt acaggcagct tagaaatttc
     4801 tgctgttatc gccgggttgc acgagtgcgt tcactgccgt acaggcagtc ttcaaactca
     4861 acactccatc ctctaatatt tattccccat aactcataga cgcaaaaaag gccggttaaa
     4921 ccgacctttt actcattctt tctcttcgcc catcaggcgg taaaacaatc agcgactacg
     4981 gaagacaatg cggcctttgc tcaggtcgta cggggtcagt tcaacagtca ctttgtcgcc
     5041 cgtcaggatg cggatgtagt ttttgcgcat tttaccggag atgtgtgcag taaccacgtg
     5101 accgttttct aactctacgc ggaacatggt attaggcaac gtttcaagaa cggtaccttg
     5161 catttcaata ttgtcttctt tggccatcta atcctctggg gtatcactac cgtaatttga
     5221 accggcaaga taatgccgaa gttctgtaaa taagtaaaga tttgcgcgct aaatcgcaac
     5281 aaacaggttc ggcacattac tccgaaaaca catggctaag ccgcaccaaa agcgcaacgt
     5341 ataagggagc ggtgagataa acgatgggcg ttacctgacg cgaaaaattc cttatcggca
     5401 gcggggtaat gagcgtaacc aactctgcga ccgcaattat aacactctgg ggagaaatgt
     5461 gccgaaaaca ttcattcttg tggtgaaaac aagcatcgag gtacccagaa attattcggc
     5521 aatcgtccga ggcgcatttg attgagataa ttaaggtaat cccggcgggg aatttcgcag
     5581 gcaccaagcg atgctgtgtg atcgttaagg acctggcagt cgataagttt accgccatga
     5641 cggataaatt catcacagaa gaccagaagc gccgttttgg acgcattttc catccggctg
     5701 aacatggact cgccacaaaa tagtgttccc tgggccacgc cgtacatacc gccgacaagc
     5761 tcatcttcac gccagacttc aatggagtgg gcatgaccga gttcgtgaag gcgatggtag
     5821 gcttcgacca cgccacgcgt gatccaggtt ccttcttcgc gatcgctggc acagccttcg
     5881 atgacctgac caaaagcgta attcatcgtg acacgatagg gtgagcgttt atgaaatcgc
     5941 ttcatactac ggctgatatg cagtgattct ggccataaca ccgcgcgggg atcgggcgac
     6001 caccagagga tggggtcgcc aggagaaaac cacgggaaaa taccacgctg ataagccatt
     6061 aataggcgcg cagggctaag atcgcccccc agtgccagca ggccgttagg ctcacgtaat
     6121 gcgccttccg gggaagggaa ggctattgaa tggcgagaaa gctgaaccag gcgcatgacc
     6181 gcaaaactcc acgcaagtcg gatcgttcaa taatagctta caaaccctgc ttaaactggt
     6241 aataacgccc ctgtctggca agcagttctg cgtgagtacc ttgctcaata atttgcccgt
     6301 tgtccatcac tattatttgt tggaaacgag agagtccgcg aagtcgatgg gtgaccatta
     6361 acaccgtttt ctcgcacatc atttctgcaa gcaattcaag gatctgactt tcggttgtgg
     6421 catctaagcc ttctgtaggt tcatccagca ataccagcgg cgcatcatgt aacagcgcac
     6481 gggcgatagc cagacggcgc agttcaccac cggagagctg gcgtccgcct tcacctaacc
     6541 aactgttgag acctgcatcc tcgagcagct tctccagacc aacgcgacgc aagatctccg
     6601 ccagagcctc atcactacta ccaggcgagg cgagtaacag attatcacgc agcgtggcgc
     6661 taaacagatg cactcgctgg ggaacaacgc tgatggtctg tcgtagagcc gcttcattca
     6721 ggctggctat gggactatcg ttaagcaaaa tctcgccctg ttgcgggtcc catgcgcggg
     6781 tcagcagttg taacagtgtt gatttgccgc atccggttcg cccgagaatc gcaatatgtt
     6841 ccccggcgtt tacctgaaga taaatccctt taagtgcctg ttgggattgc tccggataag
     6901 tgaactgaac atcccgtaat gtcagcgaaa cgagatcggc aacacgagtt tgggtatcag
     6961 ggaaggtgac ttccggtttt tgatccgtta aatcagtgat ccgtacggca gaggcaatga
     7021 cttgccccag atgctgaaat gcacccgtta ctggtgccag cgcttcaaac gcggctaacg
     7081 cgcagaagac aaacagggca attaacgcgc cgggttgagc attgccgcca acgccaccgg
     7141 acgccatcca cagcatcagg atcaccgcta acgcgccaat gagcagcatt atcgcttgcg
     7201 acaatgcggt cagttcagat tgacggcgtt gcgcttccag ccactgaatt tctgtattct
     7261 ctaattgcgt gcgataacga tcgctggcac caaaaatggt cagctcagct tgcccttgca
     7321 gccaggccgt cagttgttgg cgatactgcc cgcgaagatg agtcagattt tgcccggtgc
     7381 tttttcccgc acgataaaac agtggtggca tcaggaaaag cgtcagtaac ataatgccgc
     7441 ccagcgtaaa ggcgagggtg aaatcaagga aacttaaccc gattgtcacc accataatca
     7501 ccacaaaagc acctaccagc ggcgagataa cgcgcaggta aagatgatcg agcgtatcaa
     7561 catccgccac cacgcggttg agtaattcac cctgacgata gcgcgccagt ccggcagggg
     7621 agaggggcag caatttgctg aaggtgtata tgcgcagatg ctgcaacacg cggaaagtcg
     7681 cgtcgtgact taccagacgt tcaaaataac gcccggcagt acgggtgatt gctgcgccac
     7741 gcacgcccgc agcgggtagc atatagttga agctgtacag tccggcaacc cccgctaccg
     7801 ctgaggcgga gaggaaccag ccggaaaggg tcaacagacc gatactggcg agcagcgtga
     7861 caattgccag cacgatacca agacttaaca tccatttatg acgtttatac agtgccagat
     7921 agggtagcaa agcgcgcatt taaatctcct cctgacgatg ggccagtaat gtggcgaatg
     7981 ggccgccagc cacacttaat tccgcgtaac gtccttgctc aataatctga ccatcctgca
     8041 ttacccaaat gacatcccag tcagcaagat cttctaactg gtgggtgacc attaacgttg
     8101 tctggcgcag agaggcggca ttcagcgcct ccattacgcg ctgttcactg tgagcatcaa
     8161 ggctggcagc gggttcatcc aacagtaata gcgaacaggg atttagtaac gcacgggcca
     8221 ccgccacgcg ctgcgcctgc cccacggaaa ggcgggcagc ctggtcgcca acaggcgtat
     8281 caacgccttg tggcaggagc ggtagaaact cactgaccca ggcgttatcc agcgctgttt
     8341 gtaactcttg ttcgctggca tcaggtcgcg ccagtagtac gttatcccgt aatgttgctg
     8401 ccggtaattg tgggttttgc ccaacccagg agagatgttt acgccatgat tctggtgata
     8461 aatcgcgtaa ttctatcccg ttgattcgta gcgatccctg atatgagaga aaaccagaaa
     8521 gcgcgttcag cagtgaactt ttacctgaac cgctgcgacc aaccaacact gctcgttggc
     8581 ctgctggcaa agtaaaattc agcggtccgg ccagcgtttt accttccggc gacgtgataa
     8641 acagatcctc ggcttcaatg gtcaccggat cggtcgatgc taattccgcc tcaccgcgct
     8701 gcggatgggc gagcggggtt tccataaacg ttttcaaact gtcagctgca ccaacagcct
     8761 gggctttagc atgataaaac gtaccgagat cgcgtaatgg ctggaaaaac tctggcgcaa
     8821 ggatcagggc cagaaaaccc gcagccagcg tcacaccagt atcgtagtga ccaaaatcca
     8881 gctcgccgag gtaggaaaaa ccaaagtaga ccgccaccag agcaatcgac agcgaggtaa
     8941 aaaattcgag aatgccggag gataaaaacg ccagccgtag cacttccatt gtccgttggc
     9001 ggaaatcttc cgaagcagaa cgaatacttt caatttcagc ttcaccacga ccaaaaatac
     9061 gcaatgtttc catgccgcgc aggcgatcga ggaaatgccc acttaagcga gcaagagcga
     9121 gaaagttacg tcggttagca tcggcagccc ccattccaac cagcgccata aataacggaa
     9181 ttagcggtgc agtgcccagc agaatgagcg ccgcagccca gttagagggg aagatagcca
     9241 ccacaatcag cagcggcacc gacactgcca gcgccatttg cggcaggtag cgtgcatagt
     9301 aatcatgcat atcgtcaatt tgctcgagca ccagcgtcgc ccagctcccc gcaggtttac
     9361 cctgaatcca cgctggccct gcttgttgca gacggtcaag aacctgacgg cggatggcaa
     9421 agcggatatg ctgcccggcg tgataaccca cccgttcgcg taaccagacc acccatgcgc
     9481 gcagtacaaa ggtcagaaac agtaacgtaa agggaagcag cagggcttca cggggaatat
     9541 tctccataat catatgttgc agaattcgcg ccatgaacca ggcctgggca atgatcaata
     9601 tgccgctcac aaagcccagc agacgagaaa tattcagcca acgttgggag atgacgcttt
     9661 gctgttttaa ccagcgggtt aattcttttt gacgggattt attcattgca cgcttagcag
     9721 gtgagttatc agaattattt gcagagcaat gttacaacgg ggaaaaaata aaggcgaccc
     9781 atagtcgcat ggtgtcgcct tctttacttt tgttactgat ttgtaaaatt attttgcgtc
     9841 agctaaaccg tcgaggtagc gttccgcatc aagagctgcc atgcagcctg taccagcaga
     9901 tgtaatggcc tggcgataaa tgtgatccat cacgtcgcct gcggcaaaga cgccagggat
     9961 gctggtctgg gtggcattac catgaatacc cgactgtact ttgatgtagc cgttttccag
    10021 ttccagctgc ccttcgaaaa tggcagtatt cgggctgtga ccgatagcaa caaacagacc
    10081 ggcaacgtcg agtgactcga tgttatcgct gttttgcgta tcgcgcagac gaacgccagt
    10141 gacacccatc tgatcgccgg tcacttcttc cagcgtacgg ttggtgtgca gaataatgtt
    10201 gccgttctcc actttatcca tcagacgctt aatgaggatt ttttccgcac ggaaaccatc
    10261 acggcggtga atcagatgaa cttccgaagc gatgttagac agatacagcg cctcttcaac
    10321 cgcggtattg ccgccgccga tgaccgcaac tttctggttg cgatagaaga aaccgtcgca
    10381 ggttgcacaa gcagaaactc cacggccttt aaaggcttct tcagagggca ggccgagata
    10441 gcgtgcagaa gctccggtgg caataatcag cgcgtcgcaa gtgtattcgc cgctatcgcc
    10501 agtcagacgg aacggacgat tctgcaaatc aactttgttg atgtgatcaa agatgatttc
    10561 agtttcaaat ttggtggcat gttcgtgcat gcgcgccatt aataacggac cggtcagatc
    10621 gtttggatcg ccaggccagt tttccacttc cgtggtggtg gtcagttggc cgcctttttc
    10681 catgccggta atcagcacag gttgcaggtt ggcgcgcgcc gcgtagacag cagcggtgta
    10741 tcccgccggg cctgaaccca ggataagcag tttactgtgt ttggtcgtgc ccatgagatc
    10801 cccatagttg ttggcagaca atggcaggat tgtagggaat ttacagacgt aaaaaaagag
    10861 tatgacgatt ttgttaacaa tttgtgcaat cggcagcatc gataagcagg tcaaattctc
    10921 ccgtcattat cacctctgct acttaaattt cccgctttat aagccgatta aatgatgaat
    10981 aaacgcccct gttaatgaat atctggcatg ttgtactaaa aatcgatgtt ttgctttgac
    11041 aatcccctgg tgttttgcga aaacattcga ggaagaaaaa aaacagtatt cttatatgcg
    11101 cataaccatg catgtaaata ccatgtttac cgtgctagtg aaatctacgt atggcgtgga
    11161 cagacgccat tcgtgatgtc gatagctgcc acaaggcaac ggtcttctca ccgtagaccc
    11221 aggcattgcg cgccgtgaat cttcatgatt tcggtctatc gtgacgggta gcgactctga
    11281 acagtgatgt ttcagggtca gacaggagta gggaaggaat acagagagac aataataatg
    11341 gtagatagca agaagcgccc tggcaaagat ctcgaccgta tcgatcgtaa cattcttaat
    11401 gagttgcaaa aggatgggcg tatttctaac gtcgagcttt ctaaacgtgt gggactttcc
    11461 ccaacgccgt gccttgagcg tgtgcgtcgg ctggaaagac aagggtttat tcagggctat
    11521 acggcgctgc tgaaccccca ttatctggat gcatcacttc tggtattcgt tgagattact
    11581 ctgaatcgtg gcgcaccgga tgtgtttgaa caattcaata ccgctgtaca aaaacttgaa
    11641 gaaattcagg agtgtcattt agtatccggt gatttcgact acctgttgaa aacacgcgtg
    11701 ccggatatgt ctgcttaccg taagttactg ggggaaaccc tgctgcgtct gcctggcgtc
    11761 aatgacacac ggacatacgt tgttatggaa gaagtcaagc agagtaatcg tctggttatt
    11821 aagacgcgct aacacggaac aggtgcaaaa tcggcgtatt ttgattacac tcctgttaat
    11881 ccatacagca acagtactgg ggtaacctgg tactgttgtc cgttttagca tcgggcagga
    11941 aaagcctgta acctggagag cctttcttga gccaggaata cactgaagac aaagaagtca
    12001 cattgacaaa gttaagtagc ggtcgccgtc ttctggaggc gttgctgatc cttattgtcc
    12061 tgtttgccgt ctggttgatg gctgccttac taagttttaa cccttcggac cccagctggt
    12121 cgcaaacggc ctggcatgaa cctatccata atttaggtgg gatgcccggt gcgtggttgg
    12181 cagatactct gttctttatt tttggcgtga tggcttacac cattcccgtc attattgtcg
    12241 gcggttgttg gtttgcctgg cgtcatcagt ccagcgacga atacattgat tattttgccg
    12301 tttcgctacg catcattggc gttttggcgc tcatccttac ctcctgtggt ctggcggcaa
    12361 tcaacgctga cgatatctgg tattttgcct ccggtggcgt cattggcagc ttactaagca
    12421 ctacgctaca accactgcta cacagtagcg ggggaactat tgcgctgctc tgcgtttggg
    12481 cagcgggcct gacgctgttc accggttggt catgggtgac cattgctgaa aaactcggcg
    12541 gctggatttt aaatattctt accttcgcca gtaatcgtac ccgtcgcgat gatacctggg
    12601 tcgatgaaga tgagtatgaa gacgacgaag agtatgaaga tgaaaatcac ggcaaacagc
    12661 atgaatcacg ccgtgcccgt attcttcgcg gcgcgctagc gcgtcgtaaa cggttggcgg
    12721 aaaaattcat taatccgatg gggcggcaaa cagatgctgc gctgttctcc ggtaagcgga
    12781 tggatgatga agaagagatt acctacactg cacgcggtgt ggctgccgac cctgacgacg
    12841 tcctgttttc gggcaatcgt gcaacgcagc cagaatatga cgaatacgat ccattattaa
    12901 acggtgcgcc aattaccgaa cctgttgctg tagcagctgc tgctaccacg gcgacacaaa
    12961 gctgggctgc accggttgaa cctgtgactc agacgccgcc agttgcctct gttgatgttc
    13021 cacctacgca acctacagta gcctggcagc ctgtaccggg gccgcaaacg ggagaaccgg
    13081 ttattgcacc tgcaccggaa ggttacccac agcagtcaca atatgcgcag cctgcagtgc
    13141 aatataatga gccgctgcaa caacctgtac agccacagca gccgtattat gcgcctgcgg
    13201 ctgaacaacc tgtgcaacaa ccttactacg ctcctgcggc ggaacaacct gttcagcaac
    13261 cgtattacgc ccctgcgcca gaacaaccgg tggcaggtaa cgcctggcaa gccgaagagc
    13321 agcaatccac ttttgctcca cagtctacat accagacgga gcaaacttat cagcagccag
    13381 ctgctcagga gccgttgtac caacagccgc aacccgttga acagcagcct gttgtggagc
    13441 ctgaacccgt tgtagaagag acaaaaccca cgcgtccgcc gctttactat tttgaagaag
    13501 tggaagagaa gcgagcccgt gaacgtgaac aacttgcggc ctggtatcaa ccgattccag
    13561 aaccggttaa agaaccggaa ccgatcaaat cttcgctgaa agcaccttct gttgcagcag
    13621 tacctccagt agaagccgct gccgctgttt ctccgctggc atctggtgtg aaaaaagcga
    13681 cactggcgac gggggctgcc gcaaccgttg ccgcgccagt cttcagtctg gcaaatagcg
    13741 gtggaccgcg tccgcaggtc aaagagggga ttggcccgca gttgccacga ccgaaacgca
    13801 tccgcgtgcc aactcgtcgt gaactggcgt cttacggtat taagctgccc tcacagcgtg
    13861 cggcagaaga aaaagcccgt gaagtccagc gtaatcagta cgattctggc gatcagtaca
    13921 acgatgatga aatcgatgcg atgcagcagg atgaactggc acgtcagttc gcccagacgc
    13981 agcagcaacg ctatggcgaa cagtatcaac atgatgtgcc cgtgaacaca gaagatgcag
    14041 atgctgcggc agaggcagaa ctggctcgtc agtttgctca aactcaacaa caacgttatt
    14101 ccggcgaaca accggctggg gcgaatccgt tctcgctgga tgattttgaa ttttcgccaa
    14161 tgaaagcgtt gctggatgat ggtccacacg aaccgctgtt tacgccaatt gttgaacctg
    14221 tacagcagcc gcaacaaccg gtagcaccgc agcagcaata tcagcagccg caacaaccgg
    14281 ttgcaccgca gccgcagtat cagcagccgc aatatcagca gccacaacag ccggttgcac
    14341 agcagccgca acagccggtt gcacagcagc cgcaatatca gcagccgcaa cagccagttg
    14401 cgccgcagcc gcatgatacc ctgcttcatc cgctgttgat gcgcaatggt gacagccgtc
    14461 cgttgcataa accgacgacg ccgctgcctt ctctggattt gctgacacct ccgccgagcg
    14521 aagtggagcc ggtagatacc tttgcgcttg aacaaatggc gcgcctggta gaagcgcgtc
    14581 tggctgattt ccgtattaaa gccgatgttg tcaattactc tccggggcca gttatcactc
    14641 gctttgaatt gaacctggca ccgggcgtaa aagcagcgcg catttctaac ttgtcacgtg
    14701 accttgcccg ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac
    14761 cctatgtagg tctggagtta ccgaataaaa aacgacaaac cgtttatctg cgcgaagttt
    14821 tggataacgc caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata
    14881 tcgccggtga gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga
    14941 ctaccggttc cggtaagtct gtcggggtga acgcgatgat cctgagcatg ctttataaag
    15001 cacagccaga agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt
    15061 atgaaggcat tccgcatctg ttaacggaag tcgttaccga tatgaaagat gccgccaacg
    15121 cgctgcgctg gtgtgttaac gagatggagc gtcgttataa actgatgtct gcgctgggtg
    15181 tgcgtaatct ggcgggttat aacgaaaaaa ttgctgaagc cgataggatg atgcgtccga
    15241 ttccagaccc gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa
    15301 aagaaccgta cattgtggtg ttggtggacg aatttgccga cctgatgatg acggtaggta
    15361 aaaaagtgga agagctgata gcacgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc
    15421 tcgtactggc aacgcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata
    15481 ttccgacccg tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc
    15541 aggctggtgc ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca
    15601 cgttgccggt acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc
    15661 aggactggaa agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagtgaaa
    15721 gcgaaggtgg cgtgggcggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg
    15781 cggtgcagtt tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc
    15841 gcattggtta taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca
    15901 gcgaacaggg gcacaacggt aatcgtgaag tgctggcccc accgccattt gactaattaa
    15961 tgcaacgtag accggataag acgcgatagc gtcgcatccg gtattctatc aactgaaaat
    16021 tcagtatttt cttctttcct caagctgatt attagcctgg aatagagagt agagggaact
    16081 cccgattggg agtgacgtaa tttgaggaat aatgatgaaa aaaattgcca tcacctgtgc
    16141 attactctca agcttagtag caagcagcgt ttgggctgat gccgcaagcg atcttaaaag
    16201 ccgcctggat aaagtcagca gcttccacgc cagcttcaca cagaaagtga ctgacggtag
    16261 cggcgcggcg gtgcaggaag gtcagggcga tctgtgggtg aaacgtccaa acttattcaa
    16321 ctggcatatg actcaacctg atgaaagcat tctggtttct gacggtaaaa cactgtggtt
    16381 ctataacccg ttcgttgagc aagctacggc aacctggctg aaagatgcca ccggtaatac
    16441 gccgtttatg ctgattgccc gcaaccagtc cagcgactgg cagcagtaca atatcaaaca
    16501 gaatggcgat gactttgttc tgacgccgaa agccagcaat ggcaatctga agcagttcac
    16561 cattaacgtg ggacgtgatg gcacaatcca tcagtttagc gcggtggagc aggacgatca
    16621 gcgcagcagt tatcagctga aatcccagca aaatggggct gtggatgcag cgaaatttac
    16681 cttcaccccg ccgcaaggcg tcacggtaga tgatcaacgt aagtagaggc acctgagtga
    16741 gcaatctgtc gctcgatttt tcggataata cttttcaacc tctggccgcg cgtatgcggc
    16801 cagaaaattt agcacagtat atcggccagc aacatttgct ggctgcgggg aagccgttgc
    16861 cgcgcgctat cgaagccggg catctgcatt ctatgatcct ctgggggccg ccgggtaccg
    16921 gcaaaacaac cctcgctgaa gtgattgccc gctatgcgaa cgctgatgtg gaacgtattt
    16981 ctgccgtcac ctctggtgtg aaagagattc gcgaggcgat cgagcgcgct cggcaaaacc
    17041 gcaatgcagg tcgccgcact attctttttg ttgacgaagt tcaccgtttc aacaaaagcc
    17101 agcaggatgc atttctgcca catattgaag acggcaccat cacttttatt ggcgcaacca
    17161 ctgaaaaccc gtcgtttgag cttaattcgg cactgctttc ccgtgcccgt gtctatctgt
    17221 tgaaatccct gagtacagag gatattgagc aagtactaac tcaggcgatg gaagacaaaa
    17281 cccgtggcta tggtggtcag gatattgttc tgccagatga aacacgacgc gccattgctg
    17341 aactggtgaa tggcgacgcg cgccgggcgt taaatacgct ggaaatgatg gcggatatgg
    17401 ccgaagtcga tgatagcggt aagcgggtcc tgaagcctga attactgacc gaaatcgccg
    17461 gtgaacgtag cgcccgcttt gataacaaag gcgatcgctt ttacgatctg atttccgcac
    17521 tgcataagtc ggtacgtggt agcgcacccg atgcggcgct ctactggtat gcgcgaatta
    17581 ttaccgccgg tggcgatccg ttatatgtcg cgcgtcgttg cctggcaatt gcttccgaag
    17641 atgtcggcaa tgccgaccca cgggcgatgc aggtggcaat tgcggcctgg gattgcttta
    17701 ctcgcgttgg cccggcggaa ggtgaacgcg ccattgctca ggcgattgtt tacctggcct
    17761 gcgcgccaaa aagcaacgct gtctacactg cgtttaaagc cgcgctggcc gatgctcgcg
    17821 aacgtccgga ttatgacgtg ccggttcatt tgcgtaatgc gccgacgaaa ttaatgaagg
    17881 aaatgggcta cgggcaggaa tatcgttacg ctcatgatga agcaaacgct tatgctgccg
    17941 gtgaggttta cttcccgccg gaaatagcac aaacacgcta ttatttcccg acaaacaggg
    18001 gccttgaagg caagattggc gaaaagctcg cctggctggc tgaacaggat caaaatagcc
    18061 ccataaaacg ctaccgttaa tgttatcgtt gcggtaatgt tgttaatgta tccctgtggt
    18121 cgcaggctgt ggccacatct cccatttaat tcgataagca caggataagc atgctcgatc
    18181 ccaatctgct gcgtaatgag ccagacgcag tcgctgaaaa actggcacgc cggggcttta
    18241 agctggatgt agataagctg ggcgctcttg aggagcgtcg taaagtattg caggtcaaaa
    18301 cggaaaacct gcaagcggag cgtaactccc gatcgaaatc cattggccag gcgaaagcgc
    18361 gcggggaaga tatcgagcct ttacgtctgg aagtgaacaa actgggcgaa gagctggatg
    18421 cagcaaaagc cgagctggat gctttacagg ctgaaattcg cgatattgcg ctaaccatcc
    18481 ctaacctgcc tgcagatgaa gtgccggtag gtaaagacga aaatgacaac gttgaagtca
    18541 gtcgctgggg caccccgcgt gagtttgact ttgaagttcg tgaccatgtg acgctgggtg
    18601 aaatgcactc tggcctcgac tttgcagccg cagttaagtt gactggttcc cgctttgtgg
    18661 taatgaaagg gcagattgct cgcatgcacc gcgcactgtc gcagtttatg ctggatctgc
    18721 ataccgaaca gcatggctac agtgagaact atgttccgta cctggttaac caggacacgc
    18781 tgtacggtac gggtcagctg ccgaaatttg ctggcgatct gttccatact cgtccgctgg
    18841 aagaagaagc agacaccagt aactatgcgc tgatcccaac ggcagaagtt ccgctgacca
    18901 acctggtacg cggtgaaatc atcgatgaag atgatctgcc aattaagatg accgcccaca
    18961 ccccatgttt ccgttctgaa gctggttcat atggtcgtga cactcgtggt ctgatccgta
    19021 tgcaccagtt cgacaaagtt gaaatggtgc agatcgtgcg cccggaagac tcaatggcgg
    19081 cgctggaaga gatgactggt catgcggaaa aagtcctgca gctgctgggc ctgccgtacc
    19141 gtaaaatcat cctttgtacc ggcgacatgg gctttggcgc ttgcaaaact tacgacctgg
    19201 aagtatggat cccggcacag aacacctacc gcgagatctc ttcctgctcc aacgtttggg
    19261 atttccaggc acgtcgtatg caggcacgtt gccgtagcaa gtcggacaag aaaacccgtc
    19321 tggttcatac cctgaacggt tctggtcttg ctgttggtcg tacgctggtt gcagtaatgg
    19381 aaaactatca gcaggctgat ggtcgtattg aagtaccaga agttctgcgt ccgtatatga
    19441 acggactgga atatattggc taatacccaa tttttctgaa tctaaaaagc gcctgcgggc
    19501 gctttttttg tctccctttg ataccgaaca ataattactc ctcacttaca agtaatacta
    19561 ctttcgagtg aaaatctacc tatctctttg attttcaaat tattcgatgt atacaagcct
    19621 atatagcgat ctgctataga aataattaca caatacggtt tgttactgga atcaatcgtg
    19681 agcaagcttg agtgagccat tatgaaaacg aaaatccctg atgcggtatt ggctgctgag
    19741 gtgagtcgcc gtggtttggt aaaaacgaca gcgatcggcg gcctggcaat ggccagcagc
    19801 gcattaacat taccttttag tcggattgcg cacgctgtcg atagcgctat tccaacaaaa
    19861 tcagacgaaa aggttatctg gagcgcctgt acagttaact gtggtagtcg ctgcccgcta
    19921 cgtatgcacg tagtggacgg tgaaatcaaa tatgtcgaaa cggacaatac cggcgatgac
    19981 aattacgacg gcctgcacca ggttcgcgcc tgtctgcgtg ggcgttccat gcgtcgccgt
    20041 gtctacaatc cggaccgcct gaaatatccg atgaaacgag tcggggcgcg cggtgaaggc
    20101 aaattcgagc gcattagctg ggaagaagcc tacgacatca tcgcgaccaa aatgcagcgc
    20161 ctgatcaaag agtacggcaa cgagtctatc tatctgaact atggcaccgg tacgctgggc
    20221 ggcaccatga cccgctcgtg gccgccggga aataccctgg tcgcgcggct aatgaactgc
    20281 tgcggcggtt atctgaacca ttatggcgac tactcctccg cgcaaattgc ggaaggtctg
    20341 aactatacct acggcggctg ggcagatggt aacagcccgt cggatatcga aaacagtaaa
    20401 ctggtagtgc tgtttggtaa taaccctggc gaaacgcgaa tgagtggcgg tggggtgact
    20461 tactatcttg aacaggcgcg ccagaaatct aatgctcgca tgatcatcat cgatccgcgc
    20521 tataccgaca ccggtgccgg gcgtgaagat gagtggatcc ctattcgtcc gggaacagat
    20581 gccgcactgg ttaacggtct ggcgtatgtc atgatcaccg aaaacctggt agatcaggaa
    20641 ttcctcgata aatattgcgt tggctacgac gagaaaaccc tgccagccag tgcgccgaaa
    20701 aatggtcact ataaagctta tattctgggt gaaggacccg atggcgtggc taaaacaccg
    20761 gaatgggcct cgcaaatcac cggtgttccg gcagacaaaa tcatcaaact ggctcgtgaa
    20821 atcggcagta ccaaaccggc gtttatcagc cagggatggg gcccgcagcg tcatgctaac
    20881 ggtgaaatcg caacccgtgc tatctcgatg ctggcgattc tgaccggtaa cgttggtatt
    20941 aacgggggga acagcggcgc gcgcgaaggt tcatacagct taccgtttgt ccgtatgccg
    21001 actctcgaaa acccgatcca gaccagcatt tctatgttta tgtggaccga tgccattgaa
    21061 cgtggcccgg aaatgacggc gctgcgtgat ggtgtgcgcg ggaaagataa gctggatgtg
    21121 ccgatcaaaa tgatctggaa ctatgccggt aactgcctga ttaatcagca ttctgaaatc
    21181 aaccgtaccc atgaaatcct tcaggatgat aagaagtgcg agctgattgt ggttatcgac
    21241 tgccacatga cctcatcggc gaaatatgct gacatcctgc tgcctgactg caccgcttcc
    21301 gaacagatgg actttgcact ggatgcatcc tgcgggaata tgtcttacgt tattttcaat
    21361 gatcaggtca ttaaaccgcg ctttgaatgt aagactatct atgagatgac cagcgaactg
    21421 gcaaaacgtc ttggcgtcga acaacagttt actgaaggtc gtactcagga agagtggatg
    21481 cggcatctgt atgcccagtc gcgggaagcg attcctgaac tgccaacgtt tgaagagttc
    21541 cgtaagcagg ggatctttaa aaagcgcgat ccgcaagggc atcacgttgc ttacaaagcc
    21601 ttccgtgaag atccgcaggc taatccattg accacgccat cgggcaaaat tgagatctat
    21661 tcacaggcgc tggctgacat tgccgctacc tgggaattgc cagaaggtga tgtgatcgat
    21721 ccactgccga tctatacgcc gggctttgaa agttatcagg atccgctgaa caaacagtat
    21781 ccgctgcagc ttacgggttt ccactataaa tctcgcgttc actcaactta cggcaacgtt
    21841 gatgtgctga aagcagcttg ccgtcaggaa atgtggatca acccgcttga tgcgcaaaaa
    21901 cgcggtatca gcaacggcga taaagtcagg atctttaatg atcgtggtga ggttcatatt
    21961 gaggcgaaag tgacgccacg aatgatgccg ggtgtggtcg cactgggtga aggtgcctgg
    22021 tatgacccgg atacaaaacg tgtcgataag ggcggttgta ttaacgtact gaccactcaa
    22081 cgtccgtctc ctcttgccaa ggggaatcca tcacatacga accttgttca ggttgaaaag
    22141 gtgtaaggag taaccgatga caacccagta tggatttttt attgattcca gccgttgcac
    22201 cggttgcaaa acctgcgagc tggcttgtaa agactacaaa gatttgacgc cagaagtcag
    22261 cttccgccgc atttatgaat atgctggtgg cgactggcag gaagataacg gtgtctggca
    22321 ccagaacgtg tttgcctact atctgtcgat ttcatgtaac cactgcgaag atccggcttg
    22381 tactaaagtc tgcccgagcg gtgcgatgca taaacgtgaa gatggttttg ttgtggtcga
    22441 tgaagatgta tgcattggct gccgttactg tcatatggct tgcccgtatg gcgcaccgca
    22501 atataacgaa acgaaaggcc atatgaccaa atgcgatggt tgttatgacc gtgttgcgga
    22561 gggaaaaaaa ccgatctgcg tagagtcctg ccccctgcgg gcgctggatt tcggccctat
    22621 cgacgagctg cgtaaaaaac atggcgatct ggcggccgtt gcgccgttgc cgcgagctca
    22681 ctttaccaaa ccgaatattg tgatcaaacc caatgccaac agccgcccaa ccggggatac
    22741 cactggctat ctggcaaacc cgaaggaggt gtgagatggg aagtggatgg catgaatggc
    22801 cgctgatgat cttcacggtc ttcgggcaat gtgtagcggg tggttttatc gttctggctt
    22861 tggcgctgct caaaggcgac ctgcgagcag aagcccagca gcgtgttatc gcctgcatgt
    22921 tgggtttatg ggtgctgatg ggcattggct ttatcgcctc tatgcttcat cttggttcac
    22981 cgatgcgcgc ttttaactcg ctcaatcggg taggggcttc agcactcagt aacgaaattg
    23041 ccagcggttc gatctttttt gctgtaggcg gcatcggctg gttgctggca atgctgaaaa
    23101 agctttcacc ggcattgcgt acgctttggc tggtggtgac gatggttctt ggcgtagtct
    23161 ttgtctggat gatggtgcgt gtgtataaca gcatcgatac agtacccacc tggtacagca
    23221 tctggacacc gatgggcttc ttcctgacga tgtttatggg cggcccgtta ctgggttacc
    23281 tgttgctgag tctggctggt gtcgatggct gggcgatgcg tctgctgcca gccatttctg
    23341 ttctggcact ggtagtgagt ggcgtgatgt cagtgatgca gggcgcagag ctggcgaata
    23401 ttcatagctc tgtgcagcag gccgcagcgc tggtgccgga ctatggtgcg ctgatgtcct
    23461 ggcggatcgt gcttttggcc gttgccctgt gcttgtggat tgcaccacag ctaaaaggtt
    23521 atcagcctga ggttccgcta ctttctgtat cattcattct gcttctggca ggggaattaa
    23581 tcggtcgtgg cgtattctat ggtttgcata tgaccgtggg gatggctgtc gcaagttaat
    23641 cagtaacaac cggggtttcg gccccggttc gcttttattt ctgcttcgtt aacgtgtcat
    23701 aactggtcat caagttacga taatcaggaa tgtggttaga gaacaacgtc gccagtcctt
    23761 caatatcatt acgccagtcg cgatgcagct cgcacgccac gccgaaccat gtcatcaatt
    23821 gcgcaccagc ttgcgacatg cgatcccatg ccgaatgccg ggtaatttca ttaaaggtcc
    23881 ctgacgcgtc agtcacgaca aagacgtcaa acccttcttc aatggctgaa agcgccggga
    23941 atgccacgca aacttcggtt accacaccgg caataattaa ctgtttttta cctgtcgctt
    24001 tgacagcttt tacaaaattt tcgttatccc aggcgttaat atttcccggg cgagcaatgt
    24061 aaggtgcatc ggggaattgt gctttcagtt ctggaactaa tgggccgtta ggaccggttt
    24121 caaaactggt ggtaagaata gttggtaagt tgaaatactt agccaggtca cccagcgcca
    24181 gcacgttatt tttaaactta tcgggttcga tatcccgtac aagggaaagt aaaccagcct
    24241 ggtgatcaac aagcaaaacg gcagcatcat ttttatcaag acgaacatac ggtttggtca
    24301 tcctcttctc ctttcgagag tcagtggtac gtctaaatcg gtcagacgcg atgcattgct
    24361 ctgaaagcat agacgggaaa tatgagtttg ctgtgaccat gaaatttttc gactgaacga
    24421 agcgtatgaa atttgtgtta gatcaataaa aacaatcaga tgagattaat cgtctcttca
    24481 gcattagcgt tatgaataag taatataaat cgataatacc gccgctgcga tatgcgcgta
    24541 ttttcaccca ttgacaatgt ttttggcggt ggcatgatgc gcatgaaatt tgaacttcct
    24601 cacggtttta attcatgtcc acgtataccc ggcctgtcat gcttttgctg tctggcctgc
    24661 ttttgttgac tctggcgatt gcggtgttaa atacactcgt gccgctttgg ctcgcccagg
    24721 aacagatgtc cacatggcag gtaggcgttg tcagctcatc ctattttacc ggcaaccttg
    24781 tcggtacatt gctgacaggg tatgtcatta aacgcattgg ctttaaccgt agctattatc
    24841 tggcctcctt catttttgcc gctggctgtg ccggccttgg cctgatgatt ggtttctgga
    24901 gttggttggc gtggcgtttt gtcgcgggcg tcggctgtgc catgatttgg gtagtcgttg
    24961 agagcgcgct gatgtgcagt gggacgtcac gtaaccgtgg gcgtttgctt gctgcataca
    25021 tgatggttta ttacgtggga acgtttttag gccagttact ggtcagcaaa gtctcaaccg
    25081 agttgatgag tgtattgccg tgggttacag ctttgacgtt ggcaggaatc ttaccgctgt
    25141 tgtttacgcg tgtcctgaat cagcaggctg aaaaccatga ttcgacgtca attacgtcaa
    25201 tgctaaaact ccgtcaggcg cggcttggcg tgaatggctg cattatctca ggaatcgtgc
    25261 tgggatctct atatggcctg atgccgctgt acctcaatca caaaggggtg agcaatgcca
    25321 gcattggttt ctggatggcg gtactggtca gtgcgggtat cctcgggcaa tggccgattg
    25381 gacgtctggc ggataagttt ggtcgactgc tggtgttgcg tgttcaggtc tttgtcgtca
    25441 ttctcggcag tattgcgatg cttagccagg cggcgatggc cccagcgtta ttcatcctcg
    25501 gtgccgctgg ctttacgcta tatccggtgg cgatggcctg ggcttgtgag aaagttgaac
    25561 atcatcaact ggtggcgatg aaccaggcct tactgttgag ctataccgtg ggaagcctgc
    25621 ttggcccgtc atttaccgct atgctaatgc agaatttctc cgataattta ttatttatca
    25681 tgatcgccag cgtatcgttt atctatttgc tgatgctgct tcgtaacgcc ggtcatacgc
    25741 cgaaacccgt ggctcacgtg taaatgaatt caagcagagt gtgaacttac tgtttcacac
    25801 tctgcttttc tgtgcacaaa aaagcggcaa cttaataaag ttgccgcttt acggggaaat
    25861 tagaacatga ccttatgacc gtactgctca agaatgcctt tcacgcgttc catggtctct
    25921 ttcttcggtg gtttaacacc atcgagtttg tattcttcac tcattgccac ccatttgtgt
    25981 ttgcccagtt cgtggtaggg gaggagctcg attttctcaa cgttgcccat atcacgggta
    26041 aactcaccaa ggcgatgcgc tgaatcgtca tcgtcagacc agcctgggac aacaacatag
    26101 cggatccaca ccttcacatt tttgttcgcc agatatttag cgaactccag cgtgcggtgg
    26161 ttggaaactc caaccagatt ttggtggatc tcgtcgttca tctgtttgag atcgagcatt
    26221 accaggtcgg ttacttccag cagttcatca atcaccggat cgtaacgacg aacaaaaccg
    26281 ttggtgtcca gacaggtatg aatgccttct tttttgcagg cgcggaacca gtcacgaaca
    26341 aactcagctt gtaggattgc ctcaccgccg gatgcggtaa cgccgccgcc ggaagcgttc
    26401 ataaagtggc gataggtcac cacttccttc atcaaatctt caacggtaac ttctttaccg
    26461 ccatgcgtat cccaggtgtc gcggttatga caatacaggc agcgcatcag gcagccctgg
    26521 aaaaaggtaa taaagcgaat acccgggccg tctacggttc cacaggattc aaaggagtga
    26581 atgcgaccaa taactgacat tgcggtgttt ctccagatgt ggcccatctg aggccgtgtt
    26641 ggtgcgcagc tcgaaggcta cgtcgagtct gttttggcag tcaccttaaa gtatagatag
    26701 ctgacaaaaa aggctctcgt gctaaaaaag gccccacttt cgtggagcct ttattgtacg
    26761 ctttttactg tacgatttca gtcaaatcta attacataga ttgagtgaag gtacgagtaa
    26821 taacgtcctg ctgctgttct ttagtcagcg agttgaaacg tactgcgtag ccagatacac
    26881 ggatggtcag ctgcggatat ttttccgggt tttccatcgc gtcgagcagc atttcacggt
    26941 tcatcacgtt aacgttcagg tgctgaccac cttcgatgga tgcttcgtgg tggaagtaac
    27001 catccatcag accagccagg ttggtcttac gaacttcgtc gtctttaccc agtgcgttcg
    27061 gaacgataga gaaggtgtag gagataccat ctttagcgta agcgaacggc agtttagcaa
    27121 cggaagtcag agacgctaca gcacctttct ggtcacgacc gtgcatcggg ttagctcccg
    27181 gtccgaacgg cgcgccagca cgacgaccgt ctggggtgtt accagttttc ttaccataca
    27241 caacgttaga agtgatggtc agaacagact gagtcgggat agcgtcacgg taggtgtgca
    27301 gtttctgaat tttcttcatg aaacgttcta ccaggtcaac agccaggtca tctacacgcg
    27361 gatcgttgtt accaaactgc gggtattcgc cttcgatttc gaagtcgata gccagaccgt
    27421 cttcgtcacg aatcggttta actttcgcat atttgattgc agacagggag tcagcagcaa
    27481 cggacagacc agcgatacca cacgccatgg tgcggataac gtcacggtcg tgcagcgcca
    27541 tcagagaggc ttcgtagctg tacttgtcgt gcatgtagtg gatgatgttc agtgcagtga
    27601 tgtactgttt agccagccag tccatgaagt gatccatgcg ctccatcact tcatcatagt
    27661 tcaggacatc gcctttgatc ggttcagact tcggaccaac ctgcattttc agtttttcgt
    27721 caacgccgcc gttgattgcg tacagcatgg ttttcgccag gtttgcacgc gcaccgaaga
    27781 actgcatttg tttaccaacg atcatcgggc ttacgcagca agcaatagcg tagtcatcgt
    27841 tgttgaagtc cggacgcatc aggtcatcgt tctcatactg cagagaagag gtgtcgatgg
    27901 acactttagc ggcgaatttc ttgaagttca gcggcagttt ttcagaccac agaatggtca
    27961 tgttcggttc cggagacgga cccatggtgt acagggtgtt caggaaacgg aagctgtttt
    28021 tggtaaccag ggtacgaccg tcgaggccca taccaccgat agattcggtt gcccagatcg
    28081 ggtcgccaga gaacagttca tcgtattccg gagtacgcag gaagcgaacc atacgcagtt
    28141 tcatgaccag gtggtcaacc atttcctgcg cttcttgttc ggtgatcttg ccagctttca
    28201 ggtcacgttc gatgtacaca tccaggaagg tggaggtacg accgaaggac attgcagcac
    28261 cgttctgaga cttaacagca gccaggtagc cgaagtaagt ccactggata gcttcctgag
    28321 cgttggtagc cggaccagag atgtcgtagc cgtatttcgc agccatttct ttcatctgac
    28381 ccagagcgcg gtgctgttca gcgatttctt cgcgcagacg gatagtctgt tccaggttta
    28441 cgccgttttc cagatcagcc tgcagagagg tgaactgtgc aagtttgtct ttcatcaggt
    28501 agtcgatacc gtacagcgca acgcgacggt agtcaccgat gatacgacca cggccataag
    28561 catctggcag accggtcaga acaccggatt tacggcaacg caggatgtcc ggagtgtaaa
    28621 cgtcgaacac gccctggttg tgagttttac ggtattcagt gaagattttt ttgatcatcg
    28681 ggtccagttc gcggttgtac gctttgcagg aaccttcgat cattttgata ccaccgaacg
    28741 ggataagagc acgtttcagc ggagcttcag tctgcagacc aacgattttc tcaagctgct
    28801 tgttgatgta accagcgtcg tgagaggtga tggtggaagc aacagcggtg tcaaagtcaa
    28861 ctggcgcgtg agtgcggttt tccagtttaa cgccttccat tactttgtcc cacagggtgg
    28921 tggtcgcttc agtagcgcca gccaggaagg actcgtcacc ctcgtacgga gtgtagtttt
    28981 tctgaatgaa gtcacggacg tttacttcat tctgccagtc acctttggta aaaccttccc
    29041 aggctgtggc taacttttca ttaagctcgg acatgtaaca cctaccttct taagtggatt
    29101 ttttatttac tgcgtacttc gacaaccatt aatggtgatc gttttcacgc aggtaaatga
    29161 cccagtatgt caacccaacc aacaaaccac cgccgataat attaccgatc gtaaccggaa
    29221 tcaggttatc agtgatgaaa ttcatcacgg tcaggtgaga aaaattttcc ggtgcagaac
    29281 cgacagcggt ccagaattcc ggagatgcga agtcgcggat tacaataccc atcgggatca
    29341 taaacatgtt tgcgatactg tgctcaaaac cgctggcaac aaacatcgcg accggcagca
    29401 ccataatgaa cgctttgtcc atcaggctgc ggccagaata actcatccat actgccagac
    29461 ataccatcag gttcgccagg ataccaagac agacggcctc aataaaagta tggtgcactt
    29521 tgtggtcggc ggtttgtagg acgtttagtc cccattgacc atttgcggtc atatactcgc
    29581 cggaaagcca cattaaaagt acaaacagca gtgcgccgac caggttgcca aaataaacat
    29641 ttagccagtt tttcgccaac tgaccccagg tgatgcgccc actcgccttg gcaacaacaa
    29701 tcaacactgt ggaagtaaag agatcggctc cgcagacaac acaaagaatc aaccccagag
    29761 agaagcaaat gccgccaacc agttttgcca tgccgaaggg cattgtacct gtgccagtgg
    29821 ttgctgtgat atagaagacg aatgcgattg agatgaaaac accggcggta atcgccagat
    29881 agaaagtctt aagcggatgt ttcgttgctt tatagacacc tgcctcttcg gccactttgg
    29941 ccattgcagc aggaagtaaa agatcaaaag ggttgtcagc tttcacacta actctctctt
    30001 tattaagtcg gcgacgagat actaacaaag cattatagat gagaaattga tatagatcat
    30061 atctcgcctg gcttataggc ccgtaactcg catggttttt atgcaaatac ggagtaaata
    30121 tttgattatc caaataaaaa taaattttaa aaattaacaa atgagttgaa tttt
//
LOCUS       DAZFFE010000048        29974 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFFE010000048 DAZFFE010000000
VERSION     DAZFFE010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.048"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            161..1729
                     /gene="cydA"
                     /locus_tag="ACHJ69_002386"
     CDS             161..1729
                     /gene="cydA"
                     /locus_tag="ACHJ69_002386"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO5015358.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1745..2884
                     /gene="cydB"
                     /locus_tag="ACHJ69_002387"
     CDS             1745..2884
                     /gene="cydB"
                     /locus_tag="ACHJ69_002387"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO5015359.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            2899..3012
                     /gene="cydX"
                     /locus_tag="ACHJ69_002388"
     CDS             2899..3012
                     /gene="cydX"
                     /locus_tag="ACHJ69_002388"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO5015360.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3012..3305
                     /gene="ybgE"
                     /locus_tag="ACHJ69_002389"
     CDS             3012..3305
                     /gene="ybgE"
                     /locus_tag="ACHJ69_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO5015361.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3455..3859
                     /gene="ybgC"
                     /locus_tag="ACHJ69_002390"
     CDS             3455..3859
                     /gene="ybgC"
                     /locus_tag="ACHJ69_002390"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO5015362.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            3856..4548
                     /gene="tolQ"
                     /locus_tag="ACHJ69_002391"
     CDS             3856..4548
                     /gene="tolQ"
                     /locus_tag="ACHJ69_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO5015363.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLSGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4552..4980
                     /gene="tolR"
                     /locus_tag="ACHJ69_002392"
     CDS             4552..4980
                     /gene="tolR"
                     /locus_tag="ACHJ69_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO5015364.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5045..6310
                     /gene="tolA"
                     /locus_tag="ACHJ69_002393"
     CDS             5045..6310
                     /gene="tolA"
                     /locus_tag="ACHJ69_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO5015365.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKNQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAVEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            6443..7735
                     /gene="tolB"
                     /locus_tag="ACHJ69_002394"
     CDS             6443..7735
                     /gene="tolB"
                     /locus_tag="ACHJ69_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO5015366.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7770..8291
                     /gene="pal"
                     /locus_tag="ACHJ69_002395"
     CDS             7770..8291
                     /gene="pal"
                     /locus_tag="ACHJ69_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO5015367.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYAKNRRAVLVY"
     gene            8301..9092
                     /gene="cpoB"
                     /locus_tag="ACHJ69_002396"
     CDS             8301..9092
                     /gene="cpoB"
                     /locus_tag="ACHJ69_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO5015368.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGVAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9257..9332
                     /locus_tag="ACHJ69_002397"
     tRNA            9257..9332
                     /locus_tag="ACHJ69_002397"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9290..9292,aa:Lys,seq:ttt)
     gene            9368..9443
                     /locus_tag="ACHJ69_002398"
     tRNA            9368..9443
                     /locus_tag="ACHJ69_002398"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9401..9403,aa:Val,seq:tac)
     gene            9446..9521
                     /locus_tag="ACHJ69_002399"
     tRNA            9446..9521
                     /locus_tag="ACHJ69_002399"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9479..9481,aa:Lys,seq:ttt)
     gene            9573..9648
                     /locus_tag="ACHJ69_002400"
     tRNA            9573..9648
                     /locus_tag="ACHJ69_002400"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9606..9608,aa:Val,seq:tac)
     gene            9652..9727
                     /locus_tag="ACHJ69_002401"
     tRNA            9652..9727
                     /locus_tag="ACHJ69_002401"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9685..9687,aa:Lys,seq:ttt)
     gene            9776..9851
                     /locus_tag="ACHJ69_002402"
     tRNA            9776..9851
                     /locus_tag="ACHJ69_002402"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9809..9811,aa:Lys,seq:ttt)
     gene            9885..9960
                     /locus_tag="ACHJ69_002403"
     tRNA            9885..9960
                     /locus_tag="ACHJ69_002403"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9918..9920,aa:Lys,seq:ttt)
     gene            10238..11281
                     /gene="nadA"
                     /locus_tag="ACHJ69_002404"
     CDS             10238..11281
                     /gene="nadA"
                     /locus_tag="ACHJ69_002404"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO5015369.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGCYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVEMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALELEGSNHEVYVDERLLERALVP
                     LNRMLDFAATLRG"
     gene            11319..12038
                     /gene="pnuC"
                     /locus_tag="ACHJ69_002405"
     CDS             11319..12038
                     /gene="pnuC"
                     /locus_tag="ACHJ69_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO5015370.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(12035..12970)
                     /gene="zitB"
                     /locus_tag="ACHJ69_002406"
     CDS             complement(12035..12970)
                     /gene="zitB"
                     /locus_tag="ACHJ69_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO5015371.1"
                     /translation="MAHSHASSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGSLA
                     LLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVITIL
                     IVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHVLG
                     DLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPVSL
                     DIAELKRRLCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYLMD
                     HYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13084..13464)
                     /gene="ybgS"
                     /locus_tag="ACHJ69_002407"
     CDS             complement(13084..13464)
                     /gene="ybgS"
                     /locus_tag="ACHJ69_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO5015372.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQSNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSDGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            13780..14832
                     /gene="aroG"
                     /locus_tag="ACHJ69_002408"
     CDS             13780..14832
                     /gene="aroG"
                     /locus_tag="ACHJ69_002408"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO5015373.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLANAVNARRG"
     gene            complement(14999..15751)
                     /gene="gpmA"
                     /locus_tag="ACHJ69_002409"
     CDS             complement(14999..15751)
                     /gene="gpmA"
                     /locus_tag="ACHJ69_002409"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO5015374.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(15954..16994)
                     /gene="galM"
                     /locus_tag="ACHJ69_002410"
     CDS             complement(15954..16994)
                     /gene="galM"
                     /locus_tag="ACHJ69_002410"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO5015375.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILAEEYLPVDEGGIPHDGLKSVAGT
                     SFDFRNAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            complement(16988..18136)
                     /gene="galK"
                     /locus_tag="ACHJ69_002411"
     CDS             complement(16988..18136)
                     /gene="galK"
                     /locus_tag="ACHJ69_002411"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO5015376.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALFPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18140..19186)
                     /gene="galT"
                     /locus_tag="ACHJ69_002412"
     CDS             complement(18140..19186)
                     /gene="galT"
                     /locus_tag="ACHJ69_002412"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO5015377.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGETYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAAQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19196..20212)
                     /gene="galE"
                     /locus_tag="ACHJ69_002413"
     CDS             complement(19196..20212)
                     /gene="galE"
                     /locus_tag="ACHJ69_002413"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO5015378.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKANRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(20475..21947)
                     /gene="modF"
                     /locus_tag="ACHJ69_002414"
     CDS             complement(20475..21947)
                     /gene="modF"
                     /locus_tag="ACHJ69_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO5015379.1"
                     /translation="MSSLQILQGTFRLGDTKTLQLPRLTLNAGDSWAFVGSNGSGKSA
                     LARALSGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLSPGEDDTGRTT
                     AEIIQDEVKDTPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSELLVGVQMPEPDEPSVRHALPAHEPRIVLNNGVVSYNERPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GKTQLLFVSHHAEDAPACITHRLEFVPDGDFYRYALTKIN"
     gene            complement(22015..22803)
                     /gene="modE"
                     /locus_tag="ACHJ69_002415"
     CDS             complement(22015..22803)
                     /gene="modE"
                     /locus_tag="ACHJ69_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO5015380.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AVTAQSGARLGLDEGKEVLILLKAPWVGITQDDAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTTYFNADSVIIATLC"
     gene            22932..23081
                     /gene="acrZ"
                     /locus_tag="ACHJ69_002416"
     CDS             22932..23081
                     /gene="acrZ"
                     /locus_tag="ACHJ69_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO5015381.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23248..24021
                     /gene="modA"
                     /locus_tag="ACHJ69_002417"
     CDS             23248..24021
                     /gene="modA"
                     /locus_tag="ACHJ69_002417"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5015382.1"
                     /translation="MARKWLNLFTGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            24021..24710
                     /gene="modB"
                     /locus_tag="ACHJ69_002418"
     CDS             24021..24710
                     /gene="modB"
                     /locus_tag="ACHJ69_002418"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO5015383.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGLSFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDIKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            24713..25771
                     /gene="modC"
                     /locus_tag="ACHJ69_002419"
     CDS             24713..25771
                     /gene="modC"
                     /locus_tag="ACHJ69_002419"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO5015384.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLSPEKRRVGYVFQDARLFPHYKVRGNLRYGVA
                     KSMVNQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(25772..26590)
                     /gene="ybhA"
                     /locus_tag="ACHJ69_002420"
     CDS             complement(25772..26590)
                     /gene="ybhA"
                     /locus_tag="ACHJ69_002420"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO5015385.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYRLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQNFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            26745..27740
                     /gene="pgl"
                     /locus_tag="ACHJ69_002421"
     CDS             26745..27740
                     /gene="pgl"
                     /locus_tag="ACHJ69_002421"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO5015386.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(27781..28734)
                     /gene="ybhD"
                     /locus_tag="ACHJ69_002422"
     CDS             complement(27781..28734)
                     /gene="ybhD"
                     /locus_tag="ACHJ69_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5015387.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVQRQRHALQEIG"
     gene            28918..29970
                     /gene="ybhH"
                     /locus_tag="ACHJ69_002423"
     CDS             28918..29970
                     /gene="ybhH"
                     /locus_tag="ACHJ69_002423"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO5015388.1"
                     /translation="MEKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
BASE COUNT         7176 a         7329 c         7940 g         7529 t
ORIGIN      
        1 attcattaac acatttgtaa cttttttatc attgtctttt cctcttttga tgaaatggca
       61 acgatagagg cgagcaaata attttgtatt ggggcatgcg tgtgaccctt tctaacgggg
      121 ttcactctcg gagtcttcat gcgatgagca aggagtcatg atgttagata tagtcgaact
      181 gtcgcgctta cagtttgcct tgaccgcgat gtaccacttc ctttttgtgc cactgacgct
      241 cggtatggcg ttcctgctgg ccattatgga aacggtctac gtcctttccg gcaaacagat
      301 ttataaagat atgaccaagt tctggggcaa gttgtttggt atcaactttg ctctgggtgt
      361 ggctaccggt ctgaccatgg agttccagtt cgggactaac tggtcttact attcccacta
      421 tgtaggggat atcttcggtg cgccgctggc aatcgaaggt ctgatggcct tcttcctcga
      481 atccaccttt gtaggtctgt tcttcttcgg ttgggatcgt ctgggtaaag ttcagcatat
      541 gtgtgtcacc tggctggtgg cgctcggttc aaacctgtcc gcactgtgga ttctggttgc
      601 aaacggctgg atgcaaaacc caatcgcgtc cgatttcaac tttgaaacta tgcgtatgga
      661 gatggtgagc ttctccgagc tggtgcttaa cccggttgct caggtgaaat tcgttcacac
      721 cgtagcttct ggttatgtga ctggcgcaat gttcatcctc ggtatcagcg catggtatat
      781 gctgaaaggt cgtgacttcg ccttcgctaa acgctccttt gctatcgctg ccagcttcgg
      841 tatggctgcc gttctgtctg ttattgttct gggtgatgaa tccggctacg aaatgggcga
      901 cgtgcagaaa accaaactgg ctgctatcga agccgagtgg gaaacgcaac ctgcacctgc
      961 tgcctttact ctgttcggca ttcctgatca ggaagaggag acgaacaaat ttgcgatcca
     1021 gatcccttac gcgctgggca tcattgcaac gcgttccgtg gatactccag ttatcggcct
     1081 gaaagaactg atggtgcagc atgaagaacg cattcgtaac gggatgaagg cgtactctct
     1141 gctcgaacaa ctgcgttctg gttctaccga ccaggccgtt cgtgaccagt tcaatagcat
     1201 gaagaaagat ctcggttacg gtctgctgct gaaacgctat acgccaaacg tggctgatgc
     1261 gactgaagct cagattcaac aggcaaccaa agactccatt ccgcgtgtag cgccgctgta
     1321 cttcgcgttc cgtatcatgg tggcgtgtgg cttcctgctg ctggcaatca ttgcgctctc
     1381 tttctggagt gtcatccgca accgcattgg cgagaaaaaa tggctgctgc gcgccgcgct
     1441 gtacggtatt ccgctgccgt ggattgctgt agaagcgggc tggttcgtgg ctgaatatgg
     1501 ccgccaaccg tgggctatcg gtgaagtgct gccgacagct gtggcgaact cgtcactgac
     1561 cgcaggcgat ctcatcttct cgatggtgct gatttgcggc ctgtataccc tgttcctggt
     1621 ggcagaattg ttcttaatgt tcaagtttgc acgcctcggc ccaagcagcc tgaaaaccgg
     1681 tcgctatcac tttgagcagt cttccacgac tactcagccg gcacgctaag acaggagtcg
     1741 tcaaatgatc gattatgaag tattgcgttt tatctggtgg ctgctggttg gcgttctgct
     1801 gattggtttt gcagtcactg acggtttcga catgggggtg ggcatgctca cccgtttcct
     1861 cggtcgtaac gacaccgagc gtcgaattat gattaactcg atcgcaccac actgggacgg
     1921 taaccaggtt tggctgatca ccgcaggcgg cgcactgttt gctgcctggc cgatggtcta
     1981 tgccgctgcg ttctccggct tctatgtggc gatgatcctc gtgctggcgt ctttgttctt
     2041 ccgtccggtc ggttttgact accgctccaa gattgaagaa acccgctggc gtaacatgtg
     2101 ggactggggc atcttcattg gtagcttcgt tccgccgctg gtgattggtg tagcgttcgg
     2161 taacctgttg cagggcgtac cattcaacgt tgatgaatat ctgcgtctgt actacaccgg
     2221 taacttcttc cagctgctta acccgttcgg cctgctggca ggtgtggtga gcgtagggat
     2281 gatcattact cagggcgcaa cctatctgca aatgcgtacc gtgggcgaac tgcacctgcg
     2341 tacccgtgca acggctcagg tggcggcact ggtgacgctg gtctgcttcg cactggctgg
     2401 cgtatgggtg atgtacggta tcgatggtta tgtcgtgaaa tcgacaatgg accattacgc
     2461 agcctctaac ccgctgaata aagaagtggt tcgtgaagct ggcgcatggc tggttaactt
     2521 caacaacacg ccaattctgt gggctattcc ggcactgggt gtggttctgc cgctgctgac
     2581 catcctgact gcacgtatgg ataaagccgc gtgggcgttt gtgttctcct ccctgacgct
     2641 ggcctgcatc atcctgactg ccggtatcgc aatgttcccg tttgtgatgc cgtccagcac
     2701 catgatgaac gcaagtctga caatgtggga tgcaacttcc agccagctga cgcttaacgt
     2761 catgacctgg gttgcggtgg ttctggtacc gatcattctg ctctacaccg cctggtgtta
     2821 ctggaaaatg ttcggtcgta tcaccaaaga agatattgaa cgtaacaccc actctctgta
     2881 ctaagtaagg agctaaaaat gtggtatttc gcatggattc tgggaacgct tcttgcctgt
     2941 tcgtttgggg taatcaccgc gctggcgctt gagcacgtcg aatcaggcaa agccggtcaa
     3001 gaagacatct gatgagtaag attatcgcaa ccttgtatgc ggtaatggac aagcgcccct
     3061 tgcgggcgct ttccttcgtg atggcacttc tgttagcagg atgtatgttt tgggacccat
     3121 cacgtttcgc cgcgaagacc agtgaactgg aaatctggca tggtttactg ctgatgtggg
     3181 ccgtctgtgc tggtgtgatt cacggcgtgg gctttcgtcc gcagaaggtt ctctggcaag
     3241 ggattttttg cccattgctt gccgatattg ttctcattgt cgggctgatt ttcttcttct
     3301 tttaaatcag aattctcttt aaaaatttat gggccgagtc aggcccataa atttttacgc
     3361 tcccttaact tgccctcatt cccaaacctc aatcgcgcgc gtatagtagc agcgtttaaa
     3421 agctctaact tttgttgcat taccgggatg taaagtgaat acaacgctgt ttcgatggcc
     3481 ggttcgcgtc tactatgaag ataccgatgc cggtggtgtg gtgtaccacg ccagttacgt
     3541 cgctttttat gaaagagcac gcacagagat gctgcgtcat catcatttca gtcaacaggc
     3601 gctgatggct gaacgcgttg cctttgtggt acgtaaaatg acggtggaat attacgcacc
     3661 tgcgcggctc gacgatatgc tcgaaataca gactgaaata acatcaatgc gtggcacctc
     3721 tttggttttc acgcaacgta ttgtcaacgc cgagaatact ttgttgaatg aagcagaggt
     3781 tctggttgtt tgcgttgacc cactcaaaat gaagcctcgt gcgcttccca agtctattgt
     3841 cgcggagttt aagcagtgac tgacatgaat atccttgatt tgttcctgaa ggctagcctt
     3901 ctggttaaac ttatcatgtt gattttgatt ggtttttcaa tcgcatcctg ggccattatt
     3961 atccagcgga cccgtattct taacgcagcg gcgcgcgaag ccgaagcgtt tgaagataaa
     4021 ttctggtctg gaatcgaact ctctcgcctc tatcaagaga gccaggggaa acgggataat
     4081 ctgagcggtt cggaacaaat cttttacagc ggcttcaaag agtttgtgcg cctgcatcgt
     4141 gccaatagcc atgcgccgga agccgtagtg gaaggggcgt cgcgtgcgat gcgtatttcc
     4201 atgaatcgtg aacttgaaaa tctggaaacg cacattccgt tcctcggtac ggttggctcc
     4261 atcagcccgt atattggtct gtttggtacg gtctggggga tcatgcacgc ctttatcgcc
     4321 ctcggggcgg taaaacaagc aacactgcaa atggttgcgc ccggtatcgc agaagcgttg
     4381 attgcgacgg caattggttt gttcgccgca attccggcgg taatggctta taaccgccta
     4441 aaccagcgcg taaacaaact ggaactgaat tacgacaact ttatggaaga gtttaccgcg
     4501 attctgcacc gccaggcgtt taccgttagc gagagcaaca aggggtaagc catggccaga
     4561 gcgcgtggac gaggtcgtcg cgatctcaag tccgaaatca acattgtacc gttgctggac
     4621 gtactgctgg tgctgttgct gatctttatg gcgacagcgc ccatcatcac ccagagcgtg
     4681 gaggtcgatc tgccagacgc tactgaatca caggcggtga gcagtaacga taatccgcca
     4741 gtgattgttg aagtgtctgg tattggtcag tacaccgtgg tggttgagaa agatcgtctg
     4801 gagcgtttac caccagagca ggtggtggcg gaagtgtcca gccgtttcaa ggccaacccg
     4861 aaaacggtct ttctgatcgg tggcgcaaaa gatgtgcctt acgatgaaat aattaaagca
     4921 ctgaacttgt tacatagtgc gggtgtgaaa tcggttggtt taatgacgca gcctatctaa
     4981 acatctgcgt ttcccttgct tgaaagagag cgggtaacag gcgaacagtt tttggaaacc
     5041 gagagtgtca aaggcaaccg aacaaaacga caagctcaag cgggcgataa ttatttcagc
     5101 agtgctgcat gtcatcttat ttgcggcgct gatctggagt tcgttcgatg agaatataga
     5161 agcttcagcc ggaggcggcg gtggttcgtc catcgacgct gtcatggttg attcaggtgc
     5221 ggtagttgag cagtacaaac gtatgcaaag ccaggaatca agcgcgaagc gttctgatga
     5281 gcagcgcaag atgaaggagc agcaggctgc tgaagaactg cgtgagaaac aagcggctga
     5341 acaggaacgc ctgaagcaac ttgagaaaga gcggttagcg gctcaggagc agaaaaatca
     5401 ggctgaagaa gccgcaaaac aggccgagtt aaagcagaag caagcggaag aggcggcagc
     5461 gaaagcggcg gcagatgcta aagcgaaggc cgaagcggat gctaaagctg cggaagaagc
     5521 agcgaagaaa gcggcggcag acgcgaagaa aaaagcagaa gcagaagccg ccaaagccgc
     5581 agtcgaagcg cagaaaaaag ccgaggctgc tgctgcggcg ctgaagaaga aagctgaagc
     5641 ggcagaagca gctgcagctg aagcaagaaa gaaagcggca accgaagctg ctgaaaaagc
     5701 caaggcagaa gctgagaaga aagcggctgc tgaaaaggct gcagctgata agaaagcggc
     5761 agcagagaaa gcggcagccg acaaaaaagc agcagaaaaa gcggctgctg aaaaggcagc
     5821 agctgataag aaagcagcgg cagaaaaagc cgccgcagac aaaaaagcgg cagcggcaaa
     5881 agcagcagct gaaaaagccg ctgcagcaaa agctgccgca gaggcagatg atattttcgg
     5941 tgagctaagc tctggtaaga atgcaccgaa aacgggggga ggggcgaaag ggaacaatgc
     6001 ttcgcctgcc gggagtggta atactaaaaa caatggcgca tcaggggccg atatcaataa
     6061 ctatgccggg cagattaaat ctgctatcga aagtaagttc tatgacgcat cgtcctatgc
     6121 aggcaaaacc tgtacgctgc gcataaaact ggcacccgat ggtatgttac tggatatcaa
     6181 acctgaaggt ggcgatcccg cactttgtca agctgcgttg gcagcagcta aacttgcgaa
     6241 gatcccgaaa ccaccaagcc aggcagtata tgaagtgttc aaaaacgcgc cattggactt
     6301 caaaccgtaa tcgcgatgtt gactgttccg acggtcaaca tcaggcaccg gttgccacgg
     6361 ggttctggta gttttgtgta ttttagtttg ttaacattct gctaaattat cgtgggccat
     6421 cggtccagat aagggagata tgatgaagca ggcattacga gtagcatttg gttttctcat
     6481 actgtgggca tcagttctgc atgctgaagt ccgcattgtg atcgacagcg gtgtagattc
     6541 cggtcgtcct attggtgttg ttcctttcca gtgggcgggg cctggtgcgg cacctgaaga
     6601 tattggcggc atcgttgctg ctgacttgcg taacagcggt aaatttaatc cgttagatcg
     6661 tgctcgtctg ccacagcagc cgggtagtgc gcaggaagta caaccagctg catggtccgc
     6721 gctgggcatt gacgctgtgg ttgtcggtca ggtcactccg aatccggatg gttcttacaa
     6781 tgtcgcttat caacttgttg acactggcgg cgcaccgggt actgtacttg ctcagaactc
     6841 gtacaaagtg aacaagcagt ggctgcgtta tgctggtcat accgccagtg acgaagtatt
     6901 tgaaaaactg accggtatta aaggtgcgtt ccgtacccgt attgcctacg ttgttcagac
     6961 taacggcggt cagttcccgt atgaactgcg cgtatctgac tatgacggtt acaaccagtt
     7021 tgtcgttcac cgttcaccgc aaccgctgat gtctccggcg tggtcaccag acggttctaa
     7081 actggcttat gtgaccttcg aaagcggtcg ttccgcgctg gttattcaga cgctggcaaa
     7141 tggcgctgta cgtcaggtgg cttcattccc gcgtcacaac ggtgcacctg cattctcgcc
     7201 agacggcagc aaactggcat tcgccttgtc gaaaaccggt agcctgaacc tgtacgtaat
     7261 ggatttggct tctggtcaga tccgccaggt aactgatggt cgcagtaaca ataccgaacc
     7321 gacctggttc ccggacagcc agaacctggc atttacttct gaccaggccg gtcgtccgca
     7381 ggtttataaa gtgaatatca acggcggtgc gccacaacgt attacctggg aaggttcgca
     7441 gaaccaggat gcggatgtca gcagcgacgg taaatttatg gtaatggtca gctccaacgg
     7501 tgggcagcag cacattgcca aacaagatct ggcaacggga ggcgtacaag ttctgtcgtc
     7561 cacgttcctg gatgaaacgc caagtctggc acctaacggc actatggtaa tctacagctc
     7621 ttctcagggg atgggatccg tgctgaattt ggtttctaca gatgggcgtt tcaaagcgcg
     7681 tcttccggca actgatggac aggtcaaatt ccctgcctgg tcgccgtatc tgtgataata
     7741 attaattgaa tagtaaagga atcattgaaa tgcaactgaa caaagtgctg aaagggctga
     7801 tgattgctct gcctgttatg gcaattgcgg catgttcttc caacaagaac gccagcaatg
     7861 acggcagcga aggcatgctg ggtgccggca ctggtatgga tgcgaacggc ggcaacggca
     7921 acatgtcttc cgaagagcag gctcgtctgc aaatgcaaca gctgcagcag aacaacatcg
     7981 tttacttcga tctggacaag tacgatatcc gttctgactt cgctcaaatg ctggatgcac
     8041 acgcaaactt cctgcgtagc aacccgtctt acaaagtcac cgtagaaggt cacgcggacg
     8101 aacgtggtac tccggaatac aacatctccc tgggtgaacg tcgtgcgaac gctgttaaga
     8161 tgtatctgca gggtaaaggc gtttctgctg accagatctc catcgtttct tacggtaaag
     8221 aaaaacctgc agtactgggt catgacgaag cggcgtatgc caaaaaccgt cgtgcggtac
     8281 tggtttacta agagaattgc atgagcagta acttcagaca tcaactattg agtctgtcgt
     8341 tactggttgg tatagcggcc ccttgggccg cttttgctca ggcaccaatc agtagtgtcg
     8401 gctcaggctc ggtcgaagac cgcgtcactc aacttgagcg tatttctaat gctcacagcc
     8461 agcttttaac ccaactccag caacaactct ctgataatca atccgatatt gattccctgc
     8521 gtggtcagat tcaggaaaat cagtatcaac tgaatcaggt cgtggagcgg caaaagcaga
     8581 tcctgttgca gatcgacagc ctcagcagcg gtggtgcagc ggcgcaatca accagcggcg
     8641 atcaaagcgg tgtggcggca tcaacgacgc cgacagctga tgctggtact gcgaatgctg
     8701 gcgcgccggt gaaaagcggt gatgcaaaca cggattacaa tgcagctatt gcgctggtgc
     8761 aggataaatc ccgccaggat gacgcaatgg tggcatttca gaatttcatc aaaaattacc
     8821 ctgattcaac ttacctgcca aacgccaatt attggctggg tcagttaaac tacaacaagg
     8881 gtaaaaaaga tgatgcggcg tattattttg cttcggtagt gaaaaactat ccgaagtcac
     8941 cgaaggctgc agatgcgatg tttaaagtcg gcgtcatcat gcaggacaaa ggtgacaccg
     9001 caaaagcgaa agccgtatac cagcaggtta tcagtaaata ccctggtact gatggcgcta
     9061 aacaggcgca aaaacgtctg aacgcgatgt aatgcataac acacgaccag aagtcgcatt
     9121 atttctggtc gtgtcgtgcg aatcataagc agttgagtga tctacatcga aatttttgtt
     9181 gcgctcaagt ctgaaatcag taatatatgc cgccgttgcc acgggatatc aaacaaaccg
     9241 aaagcaacga aaaagtgggt cgttagctca gttggtagag cagttgactt ttaatcaatt
     9301 ggtcgcaggt tcgaatcctg cacgacccac cactaacata gttagttgta gtatccagcg
     9361 tagtatcggg tgattagctc agctgggaga gcacctccct tacaaggagg gggtcggcgg
     9421 ttcgatcccg tcatcaccca ccaccgggtc gttagctcag ttggtagagc agttgacttt
     9481 taatcaattg gtcgcaggtt cgaatcctgc acgacccacc actgattacg gtgggttcgg
     9541 tggaagtagt ttgtagtatc cagcgcagta tcgggtgatt agctcagctg ggagagcacc
     9601 tcccttacaa ggagggggtc ggcggttcga tcccgtcatc acccaccact cgggtcgtta
     9661 gctcagttgg tagagcagtt gacttttaat caattggtcg caggttcgaa tcctgcacga
     9721 cccaccaatt ttaaacattg gttccgagta gaaatctttc aggtaacacc cgtatgggtc
     9781 gttagctcag ttggtagagc agttgacttt taatcaattg gtcgcaggtt cgaatcctgc
     9841 acgacccacc atcctgaatg attaaagcag cataatcccg caaggggtcg ttagctcagt
     9901 tggtagagca gttgactttt aatcaattgg tcgcaggttc gaatcctgca cgacccacca
     9961 atgcaaaaaa gcgccctaaa ggcgcttttt tgctattcag gcatctctca atttcaattc
    10021 ttgaccctga tgacatactc cgcgcttgct gcgtaaacaa ctctacgtcg gaggaataag
    10081 acgcaaagtg tggcattcgg catctcatac gtaattcgag tgacttttcc ccaccattcg
    10141 actatcttgt ttagcatata aaacaaatta cgccgataat agcgaatatc acgcttaagt
    10201 cggttttaac gttaagcctg taaaacgaga tggtaagatg agcgtgatgt ttgatccaga
    10261 cacggcgatt tatcctttcc ccccgaagcc gacgccgtta agcattgatg aaaaagcgta
    10321 ttaccgcgag aagataaaac gtctactaaa agaacgtaat gcggtgatgg ttgcccacta
    10381 ctataccgat cccgaaattc aacaactggc agaagaaacc ggtggctgta tttctgattc
    10441 tctggaaatg gcgcgcttcg gtgcaaagca tcccgcttct acgttgttag ttgctggggt
    10501 gagatttatg ggagaaaccg ccaaaattct cagtccggaa aaaacaattc tgatgccgac
    10561 acttcaggct gaatgttcac tggatctcgg ctgccctgtt gaagaattta acgcattttg
    10621 cgatgcccat cccgatcgta ctgtcgtcgt ctacgccaac acttctgctg cggtaaaagc
    10681 gcgcgcagat tgggtggtaa cttcaagcat tgccgtcgaa cttattgatc atctggatag
    10741 tttgggtgaa aaaatcatct gggcacctga caaacatctg gggtgttacg tgcaaaaaca
    10801 gacgggcgca gacattttat gctggcaggg tgcctgcatt gtgcatgatg agtttaagac
    10861 tcaggcgtta acccgcttgc aagaagaata cccggatgct gccatactgg tgcatccaga
    10921 atcaccgcaa gctattgtag agatggcgga tgcggtcggt tccaccagtc aactgatcgc
    10981 tgctgcgaaa acattgccac atcagcggct tatcgtggca accgatcggg gtattttcta
    11041 caaaatgcag caggcggtgc cagataaaga gttactggaa gcaccaaccg cgggtgaggg
    11101 cgcaacctgt cgcagctgcg cgcattgccc gtggatggcc atgaatggcc ttcaggccat
    11161 cgcagaggca ttagaactgg aaggaagcaa tcacgaggtt tatgttgatg aaaggctgct
    11221 agagagggcg ctggtgccgc tcaatcgtat gctggatttt gcggctacac tacgtggata
    11281 attgataata aggcgtcaca ttacgctttg ggggaacgat ggattttttt agtgtgcaga
    11341 atatcttggt acatatacca ataggggcag gcggttatga tctctcatgg atcgaagcgg
    11401 taggcacgat cgccgggttg ctgtgtattg gccttgccag tctggagaag atcagtaact
    11461 acttctttgg cctgatcaac gtcaccttgt ttggcattat tttctttcag attcagctgt
    11521 atgccagcct gctattacag gtgtttttct ttgccgcgaa tatttacggc tggtatgcgt
    11581 ggtcgcgaca aaccagtcag aacgaggcgg agttgaaaat tcgctggttg ccattgccga
    11641 aggcgctaag ctggttggcg gtttgcgttg tttcgattgg tctgatgacg gtatttatca
    11701 atccggtgtt tgcatttttg acccgcgtgg cagtcatgat catgcaagca ttaggattac
    11761 aggttgcgat gcctgaacta cagccggacg ctttcccgtt ctgggattca tgcatgatgg
    11821 tgttatctat cgtggcaatg attctgatga cgcgtaagta tgtggaaaac tggctgttgt
    11881 gggtgattat taacgtgatt agcgtcgtta tttttgcact tcagggcgtt tacgccatgt
    11941 ctctggagta catcatcctg acctttattg ccctcaacgg cagccggatg tggatcaaca
    12001 gcgcacgcga aagaggttca cgcgcgctgt cccattaatg gtgatgatgt gaatggccag
    12061 acaccccttc attcaggtgg cagtctggcc catgacaagg ttgatattcc atctgaatag
    12121 tggcgtgctc aatctgatag tgatccatca ggtagtgttg gatctgatct aacaaggcat
    12181 cgtgatcgtg tggcgggatc acctgcacat gcagcgtcat caccggcttc tcgcctacca
    12241 tccatacatg cacatggtgc acattgcgga cttccgggat ttcccggcac aagcgacgct
    12301 tcagttcagc gatatccagc gataccggtg caccttcaag taattcattc acactatctt
    12361 tcaacagtcg ccacgcgcta cgcagaacca gaagcgacac cagtattgag agaatggggt
    12421 cagcaggtgt ccagccggtc cagataataa tcaacgcggc aataatagcc ccaaccgaac
    12481 ccagcaggtc cccgagcaca tgcagtgccg ctgctcgcac gttgaggttt ttctcttcgc
    12541 taccgtgatg aagtaaccag aaagaaagta tatttgccaa cagcccagcc acggcaattg
    12601 ccatcatcat gccgccctcg accggacgcg gtgtgcggaa gcgttctatc gcctcccaga
    12661 caatcaaaat ggtaatcacc accagggcga tagcattcac aaatgctgcg agcgtggtca
    12721 atcttagcca accgaaagtg tggcgaatgg tgggaggtcg acgtgaaaat tgcacggcga
    12781 gtagggcgaa aagcagggcg gcagtatcgg tcaacatatg acccgcatcg gccagcaatg
    12841 ccagagaacc ggaaagaaaa ccgccaatga cttctacgag cataaacccg gcagtcacgc
    12901 cgaaagcata caacaagcga cgagcattat tatcttctgg caggtgtgag gaagcgtgtg
    12961 agtgcgccat gacgagtcat cccttaatta ttctcactta actttatgac atcatactgc
    13021 ttgtagaagg gaaaaattaa aagggagaga ctccgctctc ccattattgg ctattttgca
    13081 gggttactgc gtggtaccgt cggttttggt atcgacatca ttattgatgc catcaccagt
    13141 ttgtaccttt ttattgatat ccgggcagcg accatcttta cacatggtgt tcttgtgctc
    13201 ttcatctttg gtcatgccgt cattgttcat tgaagaacca tccggatgca gcattgtgcc
    13261 gtcggaaccg gtatttaccc cgttattgtc gacgttattt ggcgcgacat tttcacgggc
    13321 gtcaggggct acctggcccg catcagctgc ggcgtttgcc tggccgttat tgctttgcgc
    13381 tccgctatcg gcagccagtg cggcaccgct ggcaaggctt agagtggcag tcaaaaatag
    13441 tgtggccagt tttgtcattt tcataggatg ctcctgttat ggtcgttatg tcggataacc
    13501 tcttccaaca gtgcatttgc aggtgaatat caggcattgg tttaagattt cagccagctt
    13561 atgaaacgca gcagagaatc ttgaaataat taacaaacaa aggagttaca gttagaaatt
    13621 gtaggagaga tctcgttttt cgcgacaatc tggcgttttt cttgctaatt ccaggattaa
    13681 tccgttcata gtgtaaaacc ccgtttacac attctgacgg aagatataga ttggaagtat
    13741 tgcattcact aagataagta tggcaacact ggaacagaca tgaattatca gaacgacgat
    13801 ttacgcatca aagaaatcaa agagttactt cctcctgtcg cattgctgga aaaattcccc
    13861 gctactgaaa atgccgcgaa tacggtcgcc catgcccgaa aagcgatcca taagatcctg
    13921 aaaggtaatg atgatcgcct gttggtggtg attggcccat gctcaattca tgatcctgtc
    13981 gcggctaaag agtatgccac tcgcttgctg gcgctgcgtg aagagctgaa agatgagctg
    14041 gaaatcgtaa tgcgcgtcta ttttgaaaag ccgcgtacca cggtgggctg gaaagggctg
    14101 attaacgatc cgcatatgga taacagcttc cagatcaacg acggtctgcg tattgcccgt
    14161 aaattgctgc ttgatattaa cgacagcggt ctgccagcgg cgggtgagtt tctcgatatg
    14221 atcaccccac aatatctcgc tgacctgatg agctggggcg caattggtgc acgtaccacc
    14281 gaatcgcagg tgcaccgcga actggcgtct ggtctttctt gtccggtagg tttcaaaaat
    14341 ggcactgatg gtacgattaa agtggctatc gatgccatta atgccgccgg tgcgccgcac
    14401 tgcttccttt cagtgaccaa atggggtcat tcggcgattg tgaataccag cggtaacggc
    14461 gattgccata tcattctgcg cggtggtaaa gagcctaact acagcgcgaa gcacgttgct
    14521 gaagtgaaag aagggctgaa caaagcaggc ctgccagcac aggtgatgat cgatttcagc
    14581 catgctaact cgtcgaaaca attcaaaaag cagatggatg tttgtgctga cgtttgccag
    14641 cagattgccg gtggcgaaaa ggccattatt ggcgtgatgg tggaaagcca tctggtggaa
    14701 ggcaatcaga gcctcgagag cggggaaccg ctggcctacg gtaagagcat caccgatgcc
    14761 tgcattggct gggatgatac cgatgctctg ttacgtcaac tggcgaatgc agtaaacgcg
    14821 cgtcgcgggt aaagtttaat tgtcggatgc gccgtcagag tggcgtatcc gattaatcac
    14881 cggaggcctg ataaaacgcg cagcgtcgca tcaggcaatg tgctccattg gtagcaacga
    14941 aaaataaaaa agccgactca ttttgcagtc ggctttctca tttttaagcg aatgacgttt
    15001 acttcgcttt accctggtta gcaaccgccg ctgctttcgc cgcgatctcg tcagcattac
    15061 ccagataata gcgtttcagc ggtttgaagt tctcgtcaaa ctcataaacc agcggcacgc
    15121 cggtcgggat attcagctca agaatctctt cttcgctcat gttatcgaga tatttcacca
    15181 gcgcacgtaa cgagttaccg tgtgcggcga tgatgacgcg ctcaccgctc ttcatacgcg
    15241 gcagaatggt ttcattccag taagggatca cgcggtcaat ggtcagcgcc aggctttccg
    15301 tcagcggcag ttctttctcg ctcagtttcg cgtaacgcgg atcgtgaccc ggataacgct
    15361 catcatcttt agtcagttcc ggcggagtca ccgcaaaacc acgacgccac tgtttcacct
    15421 gctcgtcgcc atacttttca gcggtttccg ctttgttcag accctgcaac gcaccgtagt
    15481 gacgttcgtt cagtttccag gatttctcaa cgggcagcca tgcctgatcc agttcgtcca
    15541 gcacattcca cagggtatga atagcgcgtt tcagcacaga ggtgtaagca aagtcaaagc
    15601 tgtaaccttc ctctttcagc agcttacctg ctgcttttgc ttcgcttacg cctttctcag
    15661 acagatccac gtcgtaccaa ccggtgaaac ggttttcttt gttccactga ctttcgccat
    15721 gacgaaccag aaccagctta gttacagcca tatacttact cctcaaatca tcttttaatg
    15781 ataataattc tcattatatt gccgtgacga agcaacagca atgcttacgc ataaccatag
    15841 cgaaaatagt gtcgcagtgt aaggttgttg tgaatattga gttgcaaata tgtcggtgtt
    15901 tgctggtgat ttgaacaata tgaagataaa gccctcatga cgagggctta acattactga
    15961 gcaataaact gatattccgt caggctggaa tactcttcgc caggacgcag gaagcagtcc
    16021 ggttgcggcc attcagggtg gttcgggctg tccggcagaa attcgctttc cagagccagc
    16081 ccttgccagt cggcgtaagg ttcggttccc cgcgacggtg tgccgccgag gaagttgccg
    16141 gagtagaatt gcagagccgg agcggtggtg tagaccttca gctgcaattt ttcatctgct
    16201 gaccagacat gcgccgccac tttcttgcca tcgcctttgg cctgtaacaa gaacgcgtga
    16261 tcgtaacctt tcactttgcg ctgatcgtcg tcggcaagaa actcactggc gatgattttg
    16321 gcgttgcgga aatcaaaaga ggttccggcg acagatttca ggccgtcgtg cggaatgccg
    16381 ccttcatcaa ccggcagata ttcttccgcc agaatctgca acttgtgatt gcgcacgtca
    16441 gattgctcgc cgtcaagatt gaaatagacg tgattagtca tattcaccgg gcaaggttta
    16501 tcaactgtgg cgcgataagt aatggagata cggttatcgt cggtcagacg atattgcacc
    16561 gtcgcgccga gattacccgg gaaaccctga tcgccatcat ctgaactcag ggcaaacagc
    16621 acctgacgat cgttctggtt cacaatctgc cagcgacgtt tgtcgaaccc ttccgggccg
    16681 ccatgcaatt ggttgacgcc ctgacttggt gaaagcgtca cggtttcacc gtcaaaggta
    16741 taacggctat tggcgatgcg gttggcataa cgaccaatag aggcccccag aaatgcggcc
    16801 tgatcctgat agcattccgg gctggcgcag ccaagcagcg cctcgcggac gctgccatcg
    16861 gaaagcggaa tacgggcgga aagtaaagtc gcaccccagt ccatcagcgt gactaccatc
    16921 cctgcgttgt tacgcaaagt taacagtcgg tacggctgac catcgggtgc cagtgcggga
    16981 gtttcgttca gcactgtcct gctccttgtg atggtttaca aacgtaaaaa gtctctttaa
    17041 tacctgtttt tgcttcatat tgttcagcga cagcttgctg tacggcaggc accagctctt
    17101 ccgggaacaa cgcgacgata cagccaccaa atccgccgcc ggtcatgcgt acgccacctt
    17161 tgtcgccaat cacagctttg acgatttcta ccagagtgtc aatttgcggt acggtgattt
    17221 cgaaatcatc gcgcatagag gcatgagact ccgccatcaa ctcgcccata cgtttcaggt
    17281 cgccttgctc cagtgcgctg gcagcttcaa cggtgcgggc gttttcagtc aggatatgac
    17341 gcacgcgttt cgccacgatt gggtccagtt catgcgcaac agcgttgaac tcttcaatgg
    17401 tgacatcgcg cagggctggc tgctggaaga aacgcgcacc ggtttcgcac tgttcacgac
    17461 gggtgttgta ttcgctgcca accagggtac gtttgaagtt actgttgatg atgacgacag
    17521 ccacaccttt gggcatggaa actgctttgg tccccagtga gcggcagtca atcagcaagg
    17581 catgatcttt cttgccgagt gcggaaatca gttgatccat gattccacag ttacagccta
    17641 caaactggtt ttctgcttcc tgaccattaa gcgcgatttg tgcgccgtcc agcggcagat
    17701 gataaagctg ctgcaatacg gttccgaccg cgacttccag tgaagcggaa gaacttaacc
    17761 ctgcaccctg cggcacattg ccgctgatca ccatgtccac accgccgaag ctgttgttac
    17821 gcagttgcag atgtttcacc acgccacgaa cgtagttagc ccattgatag ttttcatgcg
    17881 cgacaatggg cgcatcgagg gaaaactcgt cgagctgatt ttcataatcg gctgccatta
    17941 cgcgaacttt acggtcatcg cgtggcgcac agctgatcac ggtttgataa tcaatcgcgc
    18001 agggcagaac gaaaccgtcg ttgtagtcgg tgtgttcacc aatcaaattc acgcggccag
    18061 gcgcctgaat ggtgtgagtg gcagggtagc caaatgcgtt ggcaaacaga gattgtgttt
    18121 tttctttcag actcatttct tacactccgg attcgcgaaa atggatatcg ctgactgcgc
    18181 gcaaacgctc tgctgcctgt tctgcggtca ggtctcgctg ggtttctgcc agcatttcat
    18241 aaccaaccat aaatttacgt acggtggcgg agcgcaacag aggcggataa aagtgcgcgt
    18301 gcagctgcca gtgttgattc tcttcgccat taaatggcgc gccgtgccag cccatagagt
    18361 aggggaagga gcactggaag aggttgtcat aacgactggt tagctttttc aacgccagtg
    18421 ccaaatcgct gcgctgggcg tcggtcaaat cggtgatccg caaaacgtgg gctttgggca
    18481 gcagtagcgt ttcgaacggc caggcagccc agtaaggtac aacggctaac cagtgttcgg
    18541 tttcgacaac ggtacggcta ccgtctgcca gctcgcgctg aacatagtcc accagcattg
    18601 gcgatttctg tgcggcgaaa tattcttttt gcagacggtc ttcgcgctca gcttcgttag
    18661 gcaggaagct atttgcccaa atctgtccgt gcggatgcgg gttagagcag cccatcgccg
    18721 cgcctttgtt ttcaaagacc tgcacccatg ggtacgtttc ccccagctct gcggtttgct
    18781 cctgccaggt tttgacgatt tccgtcaatg ccgcaacgct cagttctggc aacgttttac
    18841 tgtgatccgg tgaaaagcag atcacccggc tggtaccgcg cgcgctctgg caacgcatta
    18901 gcggatcgtt gctttctggc gcatctggcg tgtcagacat caaggccgca aagtcattag
    18961 tgaaaacgta agtcccggtg taatcggggt ttttatcgcc tgtcacccgc acattacctg
    19021 cgcagaggaa gcaatctgga tcgtgcgcag gtaacacctg tttggctggc gtttcctgcg
    19081 ccccctgcca ggggcgctta gcgcggtgcg gtgaaaccag aatccattgc ccggtgagcg
    19141 ggttgtagcg gcgatgtgga tgatcaacgg gattaaattg cgtcatggtc gttccttaat
    19201 cgggatatcc ctgtggatgg cgtgactgcc agtgccaggt gtcctgcgcc atttcatcga
    19261 gtgtgcgcgt tacgcgccag ttcagttcac ggttggcttt gctggcgtcc gcccagtagg
    19321 ccggaaggtc gccctcgcga cgcggtgcaa aatgataatt aaccggtttg ccgcaggctt
    19381 tgctgaaggc attaaccacg tccagcacgc tgctgcccac gccagcgccg aggttgtaga
    19441 tgtgtacgcc tggcttgttc gccagttttt ccatcgccac gacgtgaccg tccgccagat
    19501 ccattacgtg gatgtaatcg cgcacgccag taccgtcttc ggtcggataa tcgttaccaa
    19561 aaatcgccag cgagtcgcga cggcctacag caacttgggc gatgtatggc atcaggttat
    19621 tcggaatgcc ttgcggatct tcgcccatat cgcccgacgg atgcgcgcca actgggttga
    19681 agtagcgcag cagggcaatg ctccagtccg gctgggcttt ttgcagatcg gtgaggatct
    19741 gttccaccat cagcttgctt ttgccgtaag ggctttgcgg tgtgccggtc gggaagcttt
    19801 caacatatgg aattttgggc tgatcgccat aaacggtggc ggaggagcta aagataaagt
    19861 ttttgacgtt agcggcgcgc atggcgctaa tcaggcgcag agtaccgttg acattgttgt
    19921 cgtaatattc aagcggtttt tgtaccgatt cgccaacggc tttcagcccg gcgaagtgga
    19981 tcacggtgtc gatagcgtga tcgtgcagga tctcggtcat caacgcttcg ttacggatat
    20041 cgccttcaac aaacgttgga tgtttgccgc ctaaacgctc gataacaggc agtacgctgc
    20101 gcttactgtt acagaggtta tcaagaatga tgacatcatg accgttttgc agtaattgca
    20161 cacaggtatg acttccaatg taaccgctac caccggtaac cagaactctc ataattcgct
    20221 ccattaggct tatggtatga aataaccata gcataacaaa gatgcgaaaa gtgtgacatg
    20281 gaataaatta gtggaatcgt ttacacagga atttagccgt tttttatgcg cgattaagtg
    20341 attataaaac agcggcttta tgaatgattg cgctttttat ctgaaaaaaa agacgcggtt
    20401 tcatgcctgg atgcgtcaaa ccgttggccg gataacacgt ttacgccgcc tccggcacgg
    20461 tcagcactac cgactcagtt tatttttgtc agcgcatagc gataaaagtc accgtccggc
    20521 acgaactcaa gacgatgggt aatacaggca ggcgcatctt cagcgtggtg cgaaacaaac
    20581 aacaattgcg ttttaccttc gctaatcagc acatcaacaa aacggcggat aagctggcga
    20641 ttgagcggat caagcccctg tagtggttca tcgagaataa gcaacgtcgg atgtttcact
    20701 agcgcacgga cgatcagcgc cagacgctgc tgtccccagg aaagactatg gaaaggagcg
    20761 tcagccgtgc gtttatcaat gccgagaata tccagccact gctgcaccag tttttgctgg
    20821 cgatccgaaa cggcctgata aatgccaatc gaatcaaaat agccagaaag aatcacatta
    20881 cgcacagtag tgctgacccg gtaatccaga tgcaaactac tgctgacgta accgatatgc
    20941 tttttgatat cccagatggt ttcgccgctg ccgcgacgtc gtccgaaaag cgtcaaatcg
    21001 ttgctgtaac cttgcggatg atcgccagta atcaggctta ataacgtcga ttttcccgcg
    21061 ccatttggcc cgacaatttg ccagtgttcg cctggattca cctgccagct aaggttatta
    21121 aggatggggc gttcgttata agaaaccacg ccattgttca gcacaatgcg cggttcgtgg
    21181 gcgggtaagg cgtgacgtac tgaaggttca tccggctccg gcatttgcac acctacaagc
    21241 agttcgctat gcgccagttg cgcaacgagt gcttgttgga gcagttcttc tttagcgcca
    21301 gtttccgcta acgtgcaatc cgccagcacg ccagcaaact ggacaaattc cgggatctca
    21361 tcgaagcgat tgagcaccag taccagagta ataccggact gatgtaacga ggcgagtagc
    21421 tcagccagct gctgacgtga ggcaacatcc aggccatcaa acggttcatc aaggatcaac
    21481 aagtcaggct ccgacatcag cgcctgacac agcagggttt ttcgcgtctc gccagtggaa
    21541 aggtatttaa agcgtcggtc gaggagggcg gtaataccga actgctgcgc cagttgcatg
    21601 caacgcggag tatcctttac ttcatcctga atgatctcag ccgtggtacg cccggtgtca
    21661 tcttcgccag gactaagcat atcggtgtta ttccgctgcc attcgtcgct gacgagcttt
    21721 tgcaattgct cgaaggagag acgagtgatg tgggaaaact ggctttgccg ttcacctttc
    21781 aaaagcggaa gttcccccga cagcgcgcgg gccagcgccg atttcccgct tccattcgaa
    21841 ccgacaaacg cccaactatc acccgcgttt aacgttagcc gaggcaattg cagcgttttt
    21901 gtgtcgccaa gacgaaacgt gccttgcaaa atttgcaacg atgacatttt atatcccatt
    21961 ttgtgcagcg attactgaca gggatacgtg tttcataaca aattgtcaac acgcttagca
    22021 cagcgtggcg ataatcacgc tgtcggcatt aaagtaggtc gtgacattct gcccttgctg
    22081 aagagaagtc gcttcattta ccggcactgt ggcgcacagt gtttgcccgt cgggtagcgc
    22141 cattaatact tcgcactgct ctgcgccgcg ctcaatatga ctaataatac ctggtaattg
    22201 gttgtcagcg ttttgcgcga ccgcatcgtc ctgagtaata cctacccacg gcgcttttag
    22261 caatatcaac acttctttgc cttcatccag ccccagacgc gcgccgcttt gtgcggtaac
    22321 tgcgactttc aggcgtgttt ttccatcagc cagtaagaca tcaacatgct gttgaacgtc
    22381 atcatgatcg cgggcggtga tggtgccaaa ccactggtta cgggcgctgg tttgcagtga
    22441 aaaacgtgag atcgcggcca gcaggctgtt cagcggcagg gcgtcatcgt cacttaacac
    22501 atcaaaggct ttttgctgga tttgcgccag taagtcatag agctgaatca gtcgctgacc
    22561 atagcgggtc agtactgcgc cgcctccacc tttaccgcct gttgcgcgct caaccagaat
    22621 atgctcactt aactgattca tctcgttaat ggcatcccag gcgcttttat agctgatacc
    22681 ggcatctttc gctccctggc taatggaacc ggaaagcgca atgtgtttta gtagcgaaat
    22741 gcggcgcggg tcggcgaata atttttgttg gagcttaagg gtgagaagga tttcggcctg
    22801 cataacaatg tcctggcaaa agtcttattg tgacggaaaa cgaacgccac gcaaagctga
    22861 ccgcacaaaa ggggagtgct tttctgtgct tagcggttag aatagtctca tgactatatc
    22921 tggagttgac catgttagag ttattaaaaa gtctggtatt cgccgtaatc atggtacctg
    22981 tcgtgatggc catcatcctg ggtctgattt acggtcttgg tgaagttttc aacatctttt
    23041 ctggtgttgg taaaaaagac cagcccggac aaaatcattg attccctgaa tgcccgctta
    23101 gtcgggcatt ttctttttct caacttcctg ctttttctgc cgatattttt tctcatctac
    23161 ctcacaaagg ttagcaataa ctgctgggaa aattccgaac ttgccgttat attgacgcct
    23221 acataacgtt acattaaggg gttaccaatg gctcgtaaat ggttgaactt gtttaccggg
    23281 gcggcactct ctttcgctgt tgctggcaat gcactggcag atgaagggaa aatcacggtg
    23341 ttcgccgccg catcactgac taacgcaatg caggacattg ctacgcagta taaaaaagag
    23401 aaaggcgtgg atgtggtttc ttctttcgct tcgtcatcta ctttggcccg tcagattgaa
    23461 gcgggtgcgc ctgcggatct gtttatttct gccgatcaga aatggatgga ttatgcggtt
    23521 gataaaaaag cgatcgatac ggcttcgcgc cagacactgc tcggcaatag cctggtcgtt
    23581 gtagcaccga aagccagcga gcagaaagat ttcaccatcg acagcaaaac caactggact
    23641 tcgctgctga atggcggtcg cctcgcggtt ggtgatccgg aacatgtccc tgctggcatt
    23701 tatgcgaaag aagcactgca aaaactgggc gcatgggata cgctctcccc gaaactggct
    23761 ccggcggaag atgttcgtgg cgcgctggcg ttggtcgaac gcaatgaagc tccgctgggt
    23821 attgtttacg gttctgacgc agttgccagc aaaggggtaa aagtggttgc caccttcccg
    23881 gaagattcac ataaaaaagt ggaatatccg gttgctgttg tggaagggca taacaatgcg
    23941 acagtgaaag cattttatga ttatctgaag gggccgcagg ctgcggaaat ctttaaacgt
    24001 tacggattta caaccaagta atgatactga ccgatccaga atggcaggca gttttattaa
    24061 gcctgaaagt ttcttccctg gctgtgctgt ttagcctgcc gtttgggatc ttttttgcct
    24121 ggttactggt acgttgcacg tttccgggca aagctctgct cgacagcgta ctgcatctac
    24181 cgctggtgtt accgcccgtg gtcgtcggtt acctattatt agtttcgatg ggacggcgcg
    24241 gatttatcgg tgaacgtctg tatgactggt ttggtctttc tttcgcgttt agctggcgtg
    24301 gtgcggttct cgctgccgct gtcatgtctt ttccgctgat ggtgcgggcg attcgtctgg
    24361 cgctggaagg tgttgatatc aaactggagc aggccgcaag aacactgggg gccgggcgct
    24421 ggcgcgtttt ctttactatc acgttaccgc tgaccttacc gggaattatt gttggtacgg
    24481 tgctggcatt cgctcgttcg ctcggtgaat ttggcgcaac catcaccttt gtttctaaca
    24541 ttcctggtga aacgcgaacc attccttctg ccatgtatac cctgatccag acccctggcg
    24601 gggaaagtgg cgcggcgaga ctgtgtatta tttctattgc gctggcgatg atctccctgt
    24661 tgatttcaga atggctggcc agaatcagcc gtgaacgggc ggggcgctaa tcatgctgga
    24721 actgaatttt tcccagacgt tgggcaacca ttgcctgacg attaatgaaa cgctgcccgc
    24781 caatggcatc accgctattt ttggtgtctc tggtgccgga aaaacctcgc tgattaacgc
    24841 catcagtggg ctgacacgtc cgcaaaaagg gcggattgtc ctcaatggtc gggtactaaa
    24901 tgatgccgaa aaaggtatct gcctgtcgcc ggaaaagcgt cgcgttggct atgtttttca
    24961 ggatgcacgg ctgttcccgc attacaaagt gcgtggcaat ctgcgctacg gcgtggcgaa
    25021 aagcatggtc aatcagttcg ataagctggt ggcgctttta ggcattgaac cgttgcttga
    25081 tcgtttacca ggcagcctgt ccggaggcga aaaacagcgc gtggcgattg gtcgggcttt
    25141 gctgacagca ccggaattgc tgttgctgga tgaaccgctg gcgtcactgg atattccgcg
    25201 taaacgcgaa ctgttgcctt atctgcaacg tctgacacgg gaaatcaaca ttccgatgtt
    25261 gtatgtcagc cattcgctgg atgagatcct ccatctggca gacagagtga tggtactgga
    25321 aaacggtcag gtgaaagcct ttggcgcgct ggaggaagtg tggggcagta gcgtgatgaa
    25381 tccgtggctg ccgaaagagc aacaaagtag cattctgaaa gtgacggtgc ttgagcatca
    25441 tccgcattac gcgatgaccg cgctggcgct gggcgatcag catttgtggg ttaataagct
    25501 ggacgaaccg ctgcaagctg cgctacgtat ccgcattcag gcttccgatg tttctctggt
    25561 tttacaaccg ccacagcaaa ccagcattcg taacgtattg cgggcaaaag ttgttaatag
    25621 ttatgacgac aacggccagg tggaagtgga gctggaagtc ggcggtaaaa cgctgtgggc
    25681 gcgtatcagc ccgtgggcca gggatgaact ggcgatcaaa cctggcctgt ggctgtatgc
    25741 gcaaattaaa agtgtgtcga taaccgcctg attaaatcag gtggctataa atgaactggg
    25801 caatgctgtc ggtggtgtta tcaccaatca caatgttggc gcgcgctttt actgcgtcat
    25861 cggcgttgcc catcgccacg cctgtaccag cggcttccag catactaata tcattgaagt
    25921 tatcgccgaa ggccacgacg ttttccatcg accagccttg cgcctcaacc catttcgtca
    25981 aacgtttacc tttgctgttg ccgccgcgtg caatatcaac ctgatcgtgc caggaccatt
    26041 cacactccag tcccagttca tgttcgacat gcttaccaaa attctgcaat tgcggcaggt
    26101 catcgtgcgt cagggcgaac ttccatacgg cgttaacttg ttgcgccgtt tcagccagag
    26161 aagcgacttg tgtgaaagtc ggacgctgtt ccggcggcag ggtttgcgcc cagttagacg
    26221 tgcgaatgac atgtccggtc gggtgctcat aaaccatcgc atcatccaca tacatcagac
    26281 cgtgaatgtg gtgttcattc agcatctcaa tgagttgcag ggctttgtta acaggcattg
    26341 ggtccgcttc tagtacggtt tttgcatgat aatcatacaa ataggtgccg ttacagcaaa
    26401 tagcaggtgt atccagcgcc agcgcctgat aaaaaggatg aatagcgacg tgatggcgac
    26461 ctgtgacgat gattaatcgg tagcctgctt cgcgagcacg ggccagggct tctatcgatg
    26521 aaggaagcag ggtctttttc ggggtcaata aggtgccgtc taagtcgaga gcaatcacgc
    26581 gtgtggtcat ggcgtattcc agattaaggt taagaatttt cgtctgcgcg aatggtacac
    26641 cgataccact atcggacaaa attctgcgtt ttaattcagc attcaccgcc aaaagcgact
    26701 aattttagct gttacagtca gttgctaaat gcaaaggagc attcatgaag caaacagttt
    26761 atatcgccag ccctgagagc cagcaaattc acgtctggaa cctgaatcat gaaggcgcac
    26821 tgacgctgac acaggtagtc gatgtgccgg ggcaggtgca gccgatggtg gtcagcccgg
    26881 acaaacgcta tctctatgtt ggtgttcgcc ctgagtttcg cgtcctggcg tatcgtatcg
    26941 ccccggacga tggcgcgctg acctttgctg cagagtctgc gctgccgggt agcccgacgc
    27001 atatttccac cgatcaccag gggcagtttg tctttgtcgg ttcttacaat gcgggtaacg
    27061 tgagcgtaac gcgtctggaa gatggcctgc cagtgggcgt cgtcgatgtg gtcgaggggc
    27121 tggacggttg ccattccgcc aatatctcac cggacaaccg tacgttgtgg gttccggcat
    27181 taaagcagga tcgcatttgc ctgtttacgg tcagcgatga tggtcatctg gtagcgcagg
    27241 accctgcgga agtgactacc gttgaagggg ccggcccgcg tcatatggta ttccatccaa
    27301 acgaacaata tgcgtattgc gtcaatgagt taaacagctc agtggatgtc tgggaactga
    27361 aagatccgca cggcaatatc gaatgtgtcc agacgctgga tatgatgccg gaaaacttct
    27421 ccgacacccg ttgggcggct gatattcata tcaccccgga tggtcgtcat ttatacgcct
    27481 gcgaccgtac cgccagtctg attaccgttt tcagcgtttc ggaagatggc agcgtgttga
    27541 gtaaagaagg cttccagcca acggaaaccc agccgcgcgg cttcaatgtt gatcacagcg
    27601 gcaagtatct gattgccgcc gggcaaaaat ctcaccacat ctcggtatac gaaattgttg
    27661 gcgagcaggg gctactgcat gaaaaaggcc gctatgcggt tgggcagggg ccaatgtggg
    27721 tagtggtcaa cgcgcactaa ccgctgattt aaccggcgca gtctctcctg cgccggtgta
    27781 ttaacctatc tcctgtaacg catgtctctg gcgttggacg atattggtcc acaaattgtc
    27841 tttatcgtca gtccacaaat taatcagcaa ggcaaaaaag cgttcagctg ccggagaaag
    27901 cacggcatct ttgcggcgaa taatccccaa tgtccgacgt ataacgggtt ccaccagtgg
    27961 gataccaata atcgaagaat agggcgcatg gggcattgcc agaccaggaa gcgccgaaat
    28021 ccccagtccc gcctccacca gccctaatga cgtcgaaaga tgacgcactt cgtaaaacca
    28081 gtccagcttc cagggcttgt cggctagctg ttgttctatc aatagtcggt tgccgctgga
    28141 ggagcgtacg ccaatcattt tgtaacccac taactcctgc cattctacaa gctgcttttt
    28201 ggccaatgga tggtcacgcc gacaggccag cacgaacggc tcgttgacca gtggggtaaa
    28261 atcaatggat gaatttgtga cgttgttcat gtttatgcca aagtctgatt cgttacacag
    28321 caccgattcc atgcaattat tcgtgccttg ttccagaatc cgcactttaa tattgggata
    28381 tagctcatta aatttaccga ttgccaatgg taaaaaataa aatactgcgg tcggaataca
    28441 tgctaacgtc accataccac gatgataagc attcatatca cgaatattaa aaagtgtttc
    28501 atcgaatttt tttattaatt cccgcgcctc cgggagcaac ctttttcctg cttttgttaa
    28561 cgtaaccttg cgagttgtac gctcaaatag ttgaatgtgt aaatcttcct ccatcttttt
    28621 tattcttcgc gttaatgcag gttgcgtaat attgagtaat ttagcagcat tgttaaatga
    28681 actggactct gccagtatga caaatgcctt catacttgat aattcgtgct tcattattac
    28741 tccggaaaat ggaagcgacg attttgggtg actggcagtt aaaaatttta actgcattta
    28801 gccaacttaa attaatgaaa aaatgttatt aatcgttgag ctaaagtcat tagagatgct
    28861 ttgcccttaa tgtaaccata tcgcaataag ttatgttttt aaattgaggg cattattatg
    28921 gaaaaaatac cctgcgtgat gatgcgaggt ggaacctcga ggggcgcgtt cctgttagcg
    28981 gaacatctac ccgaagatca aacgcagcgc gataaaatat tgatggcaat tatgggttcc
    29041 ggtaacgatc tggaaattga cggtattggc ggcggtaatc cgctgaccag taaagtcgcc
    29101 attattagcc gctccagcga tccgcgtgca gatgtcgatt atctgtttgc tcaagttatc
    29161 gtccatgagc aacgtgtcga taccacgcct aactgcggca atatgctgtc tggcgttggg
    29221 gcattcgcca ttgaaaatgg tttgattgca gcgacttcgc cagttacccg cgtacgtatc
    29281 cgcaacgtca atacgggtac gtttatcgaa gctgatgtgc aaacgccaaa tggtgttgtc
    29341 gagtacgagg gtagcgccag aattgacggc gtaccgggta ctgccgcacc ggttgcgctc
    29401 actttcctga atgccgccgg aacgaaaacc ggaaaagtct tcccgacgga taatcagatt
    29461 gattattttg acgatgtccc ggtgacctgt atcgatatgg cgatgccagt tgtcattatt
    29521 cctgctgaat atctgggaaa aacaggctat gaattaccgg cagagctgga tgccgacaaa
    29581 gcattattag cccgcattga atctatccgt ctacaagcgg gtaaagcaat gggcttaggc
    29641 gatgtcagta atatggttat ccctaaacct gtacttattt ctccagcgca gaaaggcggg
    29701 gcaattaatg tgcgttattt tatgccgcat tcttgccatc gcgctttggc gataaccggt
    29761 gctattgcta tttccagtag ttgtgcattg gaaggcaccg tcacccgaca aatcgtccct
    29821 tctgtaggat acggcaatat caatattgaa caccccagtg gtgcgctcga cgttcattta
    29881 agtaatgaag gtcaggatgc cacgacgtta cgcgcatctg ttattcggac gacaagaaaa
    29941 atattttccg gtgaagttta tcttccctga aaaa
//
LOCUS       DAZFFE010000049        29876 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFFE010000049 DAZFFE010000000
VERSION     DAZFFE010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.049"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..144
                     /gene="tuf"
                     /locus_tag="ACHJ69_002424"
     CDS             <1..144
                     /gene="tuf"
                     /locus_tag="ACHJ69_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO5015389.1"
                     /translation="VEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAK
                     VLS"
     gene            374..757
                     /gene="secE"
                     /locus_tag="ACHJ69_002425"
     CDS             374..757
                     /gene="secE"
                     /locus_tag="ACHJ69_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO5015390.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            759..1304
                     /gene="nusG"
                     /locus_tag="ACHJ69_002426"
     CDS             759..1304
                     /gene="nusG"
                     /locus_tag="ACHJ69_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO5015391.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEELFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1463..1891
                     /gene="rplK"
                     /locus_tag="ACHJ69_002427"
     CDS             1463..1891
                     /gene="rplK"
                     /locus_tag="ACHJ69_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO5015392.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1895..2599
                     /gene="rplA"
                     /locus_tag="ACHJ69_002428"
     CDS             1895..2599
                     /gene="rplA"
                     /locus_tag="ACHJ69_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO5015393.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2891..3388
                     /gene="rplJ"
                     /locus_tag="ACHJ69_002429"
     CDS             2891..3388
                     /gene="rplJ"
                     /locus_tag="ACHJ69_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO5015394.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3455..3820
                     /gene="rplL"
                     /locus_tag="ACHJ69_002430"
     CDS             3455..3820
                     /gene="rplL"
                     /locus_tag="ACHJ69_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO5015395.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4140..8168
                     /gene="rpoB"
                     /locus_tag="ACHJ69_002431"
     CDS             4140..8168
                     /gene="rpoB"
                     /locus_tag="ACHJ69_002431"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO5015396.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8245..12468
                     /gene="rpoC"
                     /locus_tag="ACHJ69_002432"
     CDS             8245..12468
                     /gene="rpoC"
                     /locus_tag="ACHJ69_002432"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO5015397.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYTIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12710..13843)
                     /gene="thiH"
                     /locus_tag="ACHJ69_002433"
     CDS             complement(12710..13843)
                     /gene="thiH"
                     /locus_tag="ACHJ69_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO5015398.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAEAEYAELKQLGLDGVMVYQETYHEATYAHHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVVEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQAICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRPSQRP"
     gene            complement(13840..14610)
                     /gene="thiG"
                     /locus_tag="ACHJ69_002434"
     CDS             complement(13840..14610)
                     /gene="thiG"
                     /locus_tag="ACHJ69_002434"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO5015399.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMMEAIRACGSQLVTLAMKR
                     VNLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEKLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASE"
     gene            complement(14612..14812)
                     /gene="thiS"
                     /locus_tag="ACHJ69_002435"
     CDS             complement(14612..14812)
                     /gene="thiS"
                     /locus_tag="ACHJ69_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO5015400.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQQQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(14796..15551)
                     /gene="thiF"
                     /locus_tag="ACHJ69_002436"
     CDS             complement(14796..15551)
                     /gene="thiF"
                     /locus_tag="ACHJ69_002436"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO5015401.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLMA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINATCVALNTPLITASAVRFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15544..16179)
                     /gene="thiE"
                     /locus_tag="ACHJ69_002437"
     CDS             complement(15544..16179)
                     /gene="thiE"
                     /locus_tag="ACHJ69_002437"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO5015402.1"
                     /translation="MYQPEFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDQR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLSAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16179..18074)
                     /gene="thiC"
                     /locus_tag="ACHJ69_002438"
     CDS             complement(16179..18074)
                     /gene="thiC"
                     /locus_tag="ACHJ69_002438"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO5015403.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGSAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARKGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSVQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGIITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18155..18255)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18307..18783)
                     /gene="rsd"
                     /locus_tag="ACHJ69_002439"
     CDS             complement(18307..18783)
                     /gene="rsd"
                     /locus_tag="ACHJ69_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO5015404.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGVKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDDYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKYPA"
     gene            18878..19651
                     /gene="nudC"
                     /locus_tag="ACHJ69_002440"
     CDS             18878..19651
                     /gene="nudC"
                     /locus_tag="ACHJ69_002440"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO5015405.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQLQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGEIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19691..20755
                     /gene="hemE"
                     /locus_tag="ACHJ69_002441"
     CDS             19691..20755
                     /gene="hemE"
                     /locus_tag="ACHJ69_002441"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO5015406.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVASILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20765..21436
                     /gene="nfi"
                     /locus_tag="ACHJ69_002442"
     CDS             20765..21436
                     /gene="nfi"
                     /locus_tag="ACHJ69_002442"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO5015407.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21479..22069
                     /gene="yjaG"
                     /locus_tag="ACHJ69_002443"
     CDS             21479..22069
                     /gene="yjaG"
                     /locus_tag="ACHJ69_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO5015408.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22256..22528
                     /gene="hupA"
                     /locus_tag="ACHJ69_002444"
     CDS             22256..22528
                     /gene="hupA"
                     /locus_tag="ACHJ69_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO5015409.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22541..23236
                     /gene="yjaH"
                     /locus_tag="ACHJ69_002445"
     CDS             22541..23236
                     /gene="yjaH"
                     /locus_tag="ACHJ69_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO5015410.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23238..23657)
                     /gene="zraP"
                     /locus_tag="ACHJ69_002446"
     CDS             complement(23238..23657)
                     /gene="zraP"
                     /locus_tag="ACHJ69_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO5015411.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGYGDCGGGGHMGMGHW"
     gene            23895..25271
                     /gene="zraS"
                     /locus_tag="ACHJ69_002447"
     CDS             23895..25271
                     /gene="zraS"
                     /locus_tag="ACHJ69_002447"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO5015412.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGNEEAWRRIDSADGEPVLEIYRQFQPMFATGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERASAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVELNTLISHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVN
                     ITRKDPQG"
     gene            25268..26593
                     /gene="zraR"
                     /locus_tag="ACHJ69_002448"
     CDS             25268..26593
                     /gene="zraR"
                     /locus_tag="ACHJ69_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO5015413.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKTLNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSVDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEIPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26590..27879)
                     /gene="purD"
                     /locus_tag="ACHJ69_002449"
     CDS             complement(26590..27879)
                     /gene="purD"
                     /locus_tag="ACHJ69_002449"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO5015414.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVRDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(27891..29480)
                     /gene="purH"
                     /locus_tag="ACHJ69_002450"
     CDS             complement(27891..29480)
                     /gene="purH"
                     /locus_tag="ACHJ69_002450"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO5015415.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRDQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEI
                     DANEGSLTLETRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGENKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERIPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         6989 a         7751 c         8234 g         6902 t
ORIGIN      
        1 gtagagatgg taatgccggg cgacaacatc aaaatggttg ttaccctgat ccacccgatc
       61 gcgatggacg acggtctgcg tttcgcaatc cgtgaaggcg gccgtaccgt tggcgcgggc
      121 gttgtagcaa aagttctgag ctaattgccg ataacatttg acgcaatgcg cactaaaagg
      181 gcatcatttg atgccctttt tgcacgcttt cgtaccagaa cctggctcat cagtgatttt
      241 ctttgtcata atcattgctg agacaggctc tgttgagggc gtataatccg aaaagctaat
      301 acgcgtttcg atttggtttg cctcgcgatc gcggggtgaa aatgtttgta gaatacttct
      361 gacaggttgg tttatgagtg cgaataccga agctcaagga agcgggcgcg gcctggaagc
      421 gatgaagtgg gtcgttgtgg tggcattgct cctggtagcg attgtcggca actaccttta
      481 tcgcgacatt atgctgccgc tgcgtgcgct ggccgtagta attctgattg ctgcagcggg
      541 tggtgtcgcg ctgttaacga caaaaggtaa agcaaccgtt gcttttgccc gtgaagcgcg
      601 taccgaagtc cgtaaggtca tttggccgac tcgccaggaa acattgcaca ccacgctgat
      661 tgtggctgcg gttaccgcag taatgtcact gatcctgtgg ggactggatg gtattctggt
      721 tcgcctggta tcctttatca ctggcctgag gttctgagat gtctgaagct cctaaaaagc
      781 gctggtacgt cgttcaggcg ttttccggtt ttgaaggccg cgtagcaacg tcgctgcgtg
      841 agcatatcaa attacacaac atggaagagt tgtttggtga agtcatggta ccaaccgaag
      901 aagtggttga aatccgtggc ggtcagcgtc gcaaaagcga acgtaaattc ttccctggct
      961 acgtcctcgt tcagatggtg atgaacgacg cgagctggca cctggtgcgc agcgtaccgc
     1021 gtgtgatggg cttcatcggc ggtacttccg atcgtcctgc gccaatcagc gataaagaag
     1081 tcgatgcgat tatgaaccgc ctgcagcagg ttggtgataa gccgcgtccg aaaacgttgt
     1141 ttgaaccggg tgaaatggtc cgtgttaatg atggtccgtt cgctgacttc aacggcgttg
     1201 ttgaagaagt tgattacgag aaatctcgtc tgaaagtgtc tgtttctatc ttcggtcgtg
     1261 cgacccctgt agagctggac ttcagccagg ttgaaaaagc ttaacccagc gatcaaaaaa
     1321 gcggcgattt aatcgttgca caaggcgcga gattggaata caatttcgcg ccttttgttt
     1381 ttatgggcct tgcccgtaaa acgatttttt atatcacggg gagcctctca gaggcgttat
     1441 tacccaactt gaggaattta taatggctaa gaaagtacaa gcctatgtca agctgcaggt
     1501 tgcagctggt atggctaacc cgagtccgcc agtaggtccg gctctgggtc agcagggcgt
     1561 aaacatcatg gaattctgca aagcgttcaa cgcaaaaact gattccatcg aaaaaggtct
     1621 gccgattccg gtagtaatca ctgtttacgc tgaccgttct ttcactttcg ttaccaagac
     1681 tccgccggca gcagttctgc tgaaaaaagc ggctggtatc aagtctggtt ccggtaagcc
     1741 gaacaaagac aaagtgggta aaatttcccg cgctcagctg caggaaatcg cgcagaccaa
     1801 agctgccgac atgactggtg ccgacattga agcgatgact cgctccatcg aaggtactgc
     1861 acgttccatg ggcctggtag tggaggacta agaaatggct aaactgacca agcgcatgcg
     1921 tgttatccgc gagaaagttg atgcaaccaa acagtacgac atcaacgaag ctatcgctct
     1981 gctgaaagaa ctggcgactg ctaaattcgt agaaagcgtg gacgtagctg ttaacctcgg
     2041 catcgacgct cgtaaatctg accagaacgt acgtggtgca actgtactgc cgcacggtac
     2101 tggccgttcc gttcgcgtag ccgtatttac ccagggtgca aacgctgaag ctgctaaagc
     2161 tgcaggtgct gaactggtag gtatggaaga tctggctgac cagatcaaga aaggcgaaat
     2221 gaactttgac gttgttattg cttctccgga tgcaatgcgc gttgttggcc agctgggcca
     2281 ggttctgggt ccgcgcggcc tgatgccaaa cccgaaagtg ggtactgtaa cgccgaacgt
     2341 tgctgaagcg gttaaaaacg ctaaagctgg ccaggttcgt taccgtaacg acaaaaacgg
     2401 catcatccac accaccatcg gtaaagtgga ctttgacgct gacaaactga aagaaaacct
     2461 ggaagctctg ctggttgcgc tgaaaaaagc aaaaccgact caggcgaaag gcgtgtacat
     2521 caagaaagtt agcatctcta ccaccatggg tgcaggtgtt gcagttgacc aggctggcct
     2581 gagcgcttct gtaaactaat gcctttacgt gggcggtgat tttgtctaca atcttacccc
     2641 cacgtatgct aacgaaagtt agcagctaaa aagatttgtt cgttggagcc tggcctatcc
     2701 aggcctccgt cgaagaccgc aggagtttcg caagaaactt aatcccctgc gtagacggtg
     2761 acagaacgct aagattattc ttttatattc tggcttgttt ctgctcaccg taattaagac
     2821 gctctctccg tttggaggag tgaagtgagt tccagagatt ttctctggca aacatccagg
     2881 agcaaagcta atggctttaa atcttcaaga caaacaagcg attgttgctg aagtcagcga
     2941 agtagccaaa ggcgcgctgt ctgcagtagt tgcggattcc cgtggcgtaa ctgtagataa
     3001 aatgactgaa ctgcgtaaag caggtcgcga agctggcgta tacatgcgtg ttgttcgtaa
     3061 caccctgctg cgccgtgctg ttgaaggtac tccgttcgag tgcctgaaag acgcgtttgt
     3121 tggtccgacc ctgattgcat actctatgga acacccgggc gctgctgctc gtctgttcaa
     3181 agagttcgcg aaagcgaatg caaaatttga ggtcaaagcc gctgcctttg aaggtgagct
     3241 gatcccggcg tctcagatcg accgcctggc aactctgccg acctacgaag aagcaattgc
     3301 acgcctgatg gcaaccatga aagaagcttc ggctggcaaa ctggttcgta ctctggctgc
     3361 tgtacgcgat gcgaaagaag ctgcttaatc gcagttatct ttttaacgca ttcgcttacg
     3421 tataaactta ttctgatatt caggaacaat ttaaatgtct atcactaaag atcaaatcat
     3481 tgaagcagtt gcagctatgt ctgtaatgga cgttgtagaa ctgatctctg caatggaaga
     3541 aaaattcggt gtttccgctg ctgctgctgt agctgtagct gctggcccgg ttgaagctgc
     3601 tgaagaaaaa actgaattcg acgtaattct gaaagctgct ggcgctaaca aagttgctgt
     3661 tatcaaagca gtacgtggcg caactggcct gggtctgaaa gaagctaaag acctggttga
     3721 atctgcaccg gctgctctga aagaaggcgt gagcaaagac gacgcagaag cactgaaaaa
     3781 agctctggaa gaagctggcg ctgaagttga agttaaataa gccaaccctt ccggttgcag
     3841 cctgagaaat caggctgatg gctggtgact ttttagtcac cagccttttt gcgctgtaag
     3901 gcgccagtag cgtttcacac tgtttgacta ctgctgtgcc tttcaatgct tgtttctatc
     3961 gacgacttaa tatactgcga caggacgccc gttctgtgta aatcgcaatg aaatggttta
     4021 agcgtgatag caacaggcat tgcggaaagt gttccatttt ccggtcaaca aaatagtgtt
     4081 gcacaaactg tccgctcaat ggacagatgg gtcgacttgt cagcgagctg aggaacccta
     4141 tggtttactc ctataccgag aaaaaacgta ttcgtaagga ttttggtaaa cgtccacaag
     4201 ttctggatgt accttatctc ctttctatcc agcttgactc gtttcagaaa tttatcgagc
     4261 aagatcctga agggcagtat ggtctggaag ctgctttccg ttccgtattc ccgattcaga
     4321 gctacagcgg taattccgag ctgcaatacg tcagctaccg ccttggcgaa ccggtgtttg
     4381 acgtccagga atgtcaaatc cgtggcgtga cctattccgc accgctgcgc gttaaactgc
     4441 gtctggtgat ctatgagcgc gaagcgccgg aaggcaccgt aaaagacatt aaagaacaag
     4501 aagtctacat gggcgaaatt ccgctcatga cggacaacgg tacctttgtt atcaacggta
     4561 ctgagcgtgt tatcgtttcc cagctgcacc gtagtccggg ggtctttttt gactccgaca
     4621 aaggtaaaac ccactcttcg ggtaaggtgc tgtataacgc gcgcatcatc ccttaccgtg
     4681 gttcctggct ggacttcgaa ttcgatccga aggacaacct gttcgtacgt atcgaccgtc
     4741 gccgtaaact acctgcgaca atcattctgc gtgccctgaa ctacaccaca gagcagatcc
     4801 tcgacctgtt ctttgaaaaa gttatctttg aaatccgtga taacaagttg cagatggaac
     4861 tggtgccgga acgcctgcgt ggtgaaaccg catcttttga catcgaagct aacggtaaag
     4921 tgtacgtaga aaaaggccgc cgcattactg cgcgccatat tcgccagctg gaaaaagacg
     4981 acgtcaagct gatcgaagtt ccggttgagt acatcgcagg taaagtggtt gctaaagact
     5041 acattgatga gtctaccggc gagctgatct gcgcagcgaa catggagctg agcctggatc
     5101 tgctggctaa gctgagccag tctggtcata agcgtatcga aacgctgttc accaacgatc
     5161 tggatcacgg cccatatatc tctgaaacct tacgtgtcga cccaactaac gaccgtctga
     5221 gcgcactggt agaaatctac cgtatgatgc gccctggcga gccgccgact cgtgaagctg
     5281 ctgaaagcct gttcgagaac ctgttcttct ccgaagaccg ctatgacttg tctgcggttg
     5341 gtcgtatgaa gttcaaccgt tctctgctgc gcgaagaaat cgaaggttcc ggtatcctga
     5401 gcaaagacga catcattgat gttatgaaaa agctcatcga tatccgtaac ggtaaaggcg
     5461 aagtcgatga tatcgaccac ctcggcaacc gtcgtatccg ttctgttggc gaaatggcgg
     5521 aaaaccagtt ccgcgttggc ctggtacgtg tagagcgtgc ggtgaaagag cgtctgtctc
     5581 tgggcgatct ggataccctg atgcctcagg atatgatcaa cgccaagccg atttccgcag
     5641 cagtgaaaga gttcttcggt tctagccagc tgtctcagtt tatggaccag aacaacccgc
     5701 tgtctgagat tacgcacaaa cgtcgtatct cggcactcgg cccaggcggt ctgacccgtg
     5761 aacgtgcagg cttcgaagtt cgagacgtac acccgactca ctacggtcgc gtatgtccaa
     5821 tcgaaacccc tgaaggtccg aacatcggtc tgatcaactc cctgtccgtg tacgcacaga
     5881 ctaacgaata cggcttcctt gagactccgt atcgtaaagt gaccgacggt gttgtaactg
     5941 acgaaattca ctatctgtct gctatcgaag aaggcaacta cgttatcgcc caggcgaact
     6001 ccaacctgga tgaagaaggc cacttcgtag aagacctggt aacttgccgt agcaaaggcg
     6061 aatccagctt gttcagccgc gaccaggttg actacatgga cgtatccacc cagcaggtgg
     6121 tatccgtcgg tgcgtccctg atcccgttcc tggaacacga tgacgccaac cgtgcattga
     6181 tgggtgcgaa catgcaacgt caggccgttc cgactctgcg tgctgataag ccgctggttg
     6241 gtacgggtat ggaacgtgct gttgccgttg actccggtgt aactgcggtt gctaaacgtg
     6301 gtggtgtcgt tcagtacgtg gatgcttccc gtatcgttat caaagttaac gaagacgaga
     6361 tgtatccggg tgaagcaggt atcgacatct acaacctgac caagtacacc cgttctaacc
     6421 agaacacctg tatcaaccag atgccgtgtg tgtctctggg tgaaccggta gaacgcggcg
     6481 acgtgctggc agacggtccg tccaccgatc tcggtgaact ggcgcttggt cagaacatgc
     6541 gcgtagcgtt catgccgtgg aatggttaca acttcgaaga ctccatcctc gtatccgagc
     6601 gtgttgttca ggaagaccgt ttcaccacca tccatattca ggaactggcg tgtgtgtccc
     6661 gtgataccaa gctggggccg gaagagatca ccgctgacat cccgaacgtg ggtgaagctg
     6721 ctctctccaa actggatgaa tccggtatcg tttacattgg tgcggaagtg accggtggcg
     6781 acattctggt tggtaaggta acgccgaaag gtgaaactca gctgacccca gaagaaaaac
     6841 tgctgcgtgc gatcttcggt gagaaagcgt ctgacgttaa agactcttct ctgcgcgtac
     6901 caaacggtgt atccggtacg gttatcgatg ttcaggtctt tactcgcgat ggcgtagaaa
     6961 aagacaaacg tgcgctggaa atcgaagaaa tgcagctcaa acaggcgaag aaagacctgt
     7021 ctgaagaact gcagatcctc gaagcaggtc tgttcagccg tatccgtgct gtgctggtag
     7081 ccggtggcgt tgaagctgag aaactcgaca aactgccgcg cgatcgctgg ctggagctgg
     7141 gcctgaccga cgaagagaaa caaaatcagc tggaacagct ggctgagcag tatgacgaac
     7201 tgaaacacga gttcgagaag aaactcgaag cgaaacgccg caaaatcacc cagggcgacg
     7261 atctggcacc gggcgtgctg aagattgtta aggtatatct ggcggttaaa cgccgtatcc
     7321 agcctggtga caagatggca ggccgtcatg gtaacaaggg tgtaatttct aagatcaacc
     7381 cgatcgaaga tatgccttac gatgaaaacg gtactccggt agacatcgta ctgaacccgc
     7441 tgggcgtacc gtctcgtatg aacattggtc agatcctcga aacccaccta ggtatggctg
     7501 cgaaaggtat cggcgacaag atcaacgcca tgctgaaaca gcagcaagaa gtcgcgaaac
     7561 tgcgcgaatt catccagcgt gcgtacgatc tgggcgctga tgttcgtcag aaagttgacc
     7621 tgagtacctt cagcgatgaa gaagttatgc gtctggctga aaacctgcgc aaaggtatgc
     7681 caatcgcaac gccggtcttc gacggtgcga aagaagcaga aattaaagag ctgctgaaac
     7741 ttggcgacct gccgacttct ggtcagatcc gcctgtacga cggtcgcact ggtgaacagt
     7801 tcgagcgtcc ggtaaccgtt ggttacatgt acatgctgaa actgaaccac ctggtcgacg
     7861 acaagatgca cgcgcgttcc accggttctt acagcctggt tactcagcag ccgctgggtg
     7921 gtaaggcaca gttcggtggt cagcgtttcg gggagatgga agtgtgggcg ctggaagcat
     7981 atggcgcagc atacaccctg caggaaatgc tcaccgttaa gtctgatgac gtgaacggtc
     8041 gtactaagat gtataaaaac atcgtggacg gcaaccatca gatggagccg ggcatgccag
     8101 aatccttcaa cgtattgttg aaagagattc gttcgctggg tatcaacatc gaactggaag
     8161 acgagtaatt ctcgctcaaa caggtcactg ctgtcggggt aaaacccggc agcggattgt
     8221 gctaactccg acgggagcaa atccgtgaaa gatttattaa agtttctgaa agcgcagact
     8281 aaaaccgaag agtttgatgc gatcaaaatt gctctggctt cgccagacat gatccgttca
     8341 tggtctttcg gtgaagttaa aaagccggaa accatcaact accgtacgtt caaaccagaa
     8401 cgtgacggcc ttttctgcgc ccgtatcttt gggccggtaa aagattacga gtgcctgtgc
     8461 ggtaagtaca agcgcctgaa acaccgtggc gtcatctgtg agaagtgcgg cgttgaagtg
     8521 acccagacta aagtacgccg tgagcgtatg ggccacatcg aactggcttc cccgactgcg
     8581 cacatctggt tcctgaaatc gctgccgtcc cgtatcggcc tgctgctcga tatgccgctg
     8641 cgcgatatcg aacgcgtact gtacttcgaa tcctatgtgg ttatcgaagg cggtatgacc
     8701 aacctggaac gtcagcagat cctgactgaa gagcagtatc tggacgcgct ggaagagttc
     8761 ggtgacgaat tcgacgcgaa gatgggggcg gaagcaatcc aggccctgct gaagagcatg
     8821 gatctggagc aagagtgcga acagctgcgt gaagagctga acgaaaccaa ctccgaaacc
     8881 aagcgtaaaa agctgaccaa gcgtatcaaa ctgctggaag cgttcgttca gtctggtaac
     8941 aaaccagagt ggatgatcct gaccgttctg ccggtactgc cgccagatct gcgtccgctg
     9001 gttccgctgg atggtggtcg tttcgcgact tctgacctga acgatctgta tcgtcgcgtc
     9061 attaaccgta acaaccgtct gaaacgacta ctggacctgg ctgcgccgga tatcatcgta
     9121 cgtaacgaaa aacgtatgct gcaggaagcg gtagacgccc tgctggataa cggtcgtcgc
     9181 ggtcgtgcaa tcaccggttc taacaagcgt cctctgaaat ctttggccga catgatcaaa
     9241 ggtaaacagg gtcgtttccg tcagaacctg ctcggtaagc gcgttgacta ctccggtcgt
     9301 tctgtaatca ccgtaggtcc atacctgcgt ctgcatcagt gcggtctgcc gaagaaaatg
     9361 gcgctggagc tgttcaaacc gttcatctac ggcaagctgg aactgcgtgg tcttgctacc
     9421 accatcaaag ctgcgaagaa aatggttgag cgcgaagaag ctgtcgtttg ggatatcctg
     9481 gacgaagtga tccgcgaaca cccggtactg ctgaaccgtg caccgactct gcaccgtctg
     9541 ggtatccagg catttgaacc ggtactgatc gaaggtaaag ctatccagct gcacccgctg
     9601 gtttgtgcgg catataacgc cgacttcgat ggtgaccaga tggctgttca cgtaccgctg
     9661 acgctggaag cccagctcga agcgcgtgcg ctgatgatgt ctaccaacaa catcctgtcc
     9721 ccggcgaacg gcgaaccaat catcgttccg tctcaggacg ttgtactggg tctgtactac
     9781 atgacccgcg actgtgttaa cgccaaaggc gaaggcatgg tgctgactgg cccgaaagaa
     9841 gcagaacgtc tgtatcgctc tggtctggct tctctgcatg cgcgcgttaa agtgcgtatc
     9901 accgagtatg aaaaagatgc taacggtgaa ttagtggcga aaaccagcct gaaagacacg
     9961 actgttggcc gtgccattct gtggatgatt gtaccgaaag gtctgcctta caccatcgtc
    10021 aaccaggcgc tgggtaaaaa agcaatctcc aaaatgctga acacctgcta ccgcattctc
    10081 ggtctgaaac cgactgttat ttttgcggac cagatcatgt ataccggctt tgcatatgca
    10141 gcgcgttctg gtgcatctgt tggtatcgat gacatggtca tcccggagaa gaaacacgaa
    10201 atcatctccg aggcagaagc agaagttgct gaaattcagg agcagttcca gtctggtctg
    10261 gtaactgcgg gcgaacgcta caacaaagtt atcgatatct gggctgcggc gaacgatcgt
    10321 gtatccaaag cgatgatgga taacctgcaa actgaaaccg ttattaaccg tgacggtcag
    10381 gaagagaagc aggtttcctt caacagcatc tacatgatgg ccgactccgg tgcgcgtggt
    10441 tctgcggcac agattcgtca gcttgctggt atgcgtggtc tgatggcgaa gccggatggc
    10501 tccatcatcg aaacgccaat caccgcgaac ttccgtgaag gtctgaacgt actccagtac
    10561 ttcatctcca cccacggtgc tcgtaaaggt ctggcggata ccgcactgaa aactgcgaac
    10621 tccggttacc tgactcgtcg tctggttgac gtggcgcagg acctggtggt taccgaagac
    10681 gattgtggta ctcatgaagg tatcatgatg actccggtta tcgaaggtgg tgacgttaaa
    10741 gagccgctgc gcgatcgcgt attgggtcgt gtaactgctg aagacgttct gaagccgggt
    10801 actgctgata tcctcgttcc gcgcaacacg ctgctgcacg aacagtggtg tgacctgctg
    10861 gaagagaact ctgtcgacgc ggttaaagta cgttctgttg tatcttgtga caccgacttt
    10921 ggtgtatgtg cgcactgcta cggtcgtgac ctggcgcgtg gccacatcat taacaaaggt
    10981 gaagcaatcg gtgttatcgc ggcacagtcc atcggtgaac cgggtacaca gctgaccatg
    11041 cgtacgttcc acatcggtgg tgcggcatct cgtgcggctg ctgaatccag catccaggtg
    11101 aaaaacaaag gtagcatcaa gctcagcaac gtgaagtcgg ttgtgaactc cagcggtaaa
    11161 ctggttatca cttcccgtaa caccgaactg aaactgatcg acgaattcgg tcgtaccaaa
    11221 gaaagctaca aagtacctta cggtgcggta ctggcgaaag gcgatggcga acaggttgct
    11281 ggcggcgaaa ccgttgcaaa ctgggacccg cacaccatgc cggttatcac cgaagtaagc
    11341 ggttttgtac gctttactga catgatcgac ggccagacca ttactcgtca gaccgacgaa
    11401 ttgacaggtc tgtcttcgct ggtggttctg gattccgcag aacgtaccgc aggtggtaaa
    11461 gatctgcgtc cggcactgaa aatcgttgat gctcagggta acgacgttct gatcccgggt
    11521 accgatatgc ctgcgcagta cttcctgccg ggtaaagcga ttgttcagct ggaagatggc
    11581 gtacagatca gctctggtga caccctggcg cgtattccgc aggaatccgg cggtaccaag
    11641 gacatcaccg gtggtctgcc gcgcgttgcg gacctgttcg aagcacgtcg tccgaaagag
    11701 ccggcaatcc tggctgaaat cagcggtatc gtttccttcg gtaaagaaac caaaggtaaa
    11761 cgtcgtctgg ttatcacccc ggtagacggt agcgatccgt acgaagagat gattccgaaa
    11821 tggcgtcagc tcaacgtgtt cgaaggtgaa cgtgtagaac gtggtgacgt aatttccgac
    11881 ggtccggaag cgccgcacga cattctgcgt ctgcgtggtg ttcatgctgt gactcgttac
    11941 atcgttaacg aagtacagga cgtataccgt ctgcagggcg ttaagattaa cgataaacac
    12001 atcgaagtta tcgttcgtca gatgctgcgt aaagctacca tcgttaacgc gggcagctcc
    12061 gacttcctgg aaggtgaaca ggttgaatac tctcgcgtca agatcgcaaa ccgcgaactg
    12121 gaagcgaacg gcaaagtggg tgcaacttac tcccgcgatc tgctgggtat caccaaagcg
    12181 tctctggcaa ccgagtcctt catctccgcg gcatcgttcc aggagaccac tcgcgtgctg
    12241 accgaagcag ccgttgcggg caaacgcgac gaactgcgcg gcctgaaaga gaacgttatc
    12301 gtgggtcgtc tgatcccggc aggtaccggt tacgcgtacc accaggatcg tatgcgtcgc
    12361 cgtgctgcgg gtgaagctcc ggctgcaccg caggtgactg cagaagacgc atctgccagc
    12421 ctggcagaac tgctgaacgc aggtctgggt ggttctgata acgagtaatc gttaatccgc
    12481 aaataacgta aaaacccgct tcggcgggtt tttttatggg tgttattcag agggcaaagt
    12541 cttcacggaa aatattaaga aaggcagggc gaaacgagga agaagctttt cgccctggac
    12601 aacattcctg ctgacggcac taccataaaa aaacacaacg ctttcaacaa ccttgcgttt
    12661 ttcatcttcc gtagcggctc ggaataaatt tacgttctta catactgtct catggccttt
    12721 gcgatgggcg tcccagatag ctgtcccagt ctttccatac cggctgcaaa ccctgagcgg
    12781 ttaacgcggc agcaaccgct tccggtctgc gatcgtcgtg cggtgagaac tgttccagct
    12841 cggggtggtt gtcggcatag ccacctggct gcgttttcga aaaagcgctg acgttattaa
    12901 ttgccagcgg aatcacgcga tcgcgaaacc acggtgattc ccgcgtggag agtgacagtt
    12961 caatctccgg tgcaagcagc cggaaagcgc agatggcttg cactaactgg cgttcatcca
    13021 taatcgacgc aggctcaatg ccgccagtac atggacgcag gcgtgggaag gagacagagt
    13081 agcggctttg ccagtaatgc tgttgcagcc atagcaaatg ttcgacaacc atatagcagt
    13141 caactcgcca gctgtcggaa aggccgatta gcgcgccgag gcctatctta tcaatccccg
    13201 cacgccctag ccgatccggc gtttccagcc gccagaagaa gtcctgtttt ttacctttca
    13261 gatgatgatg ggcataagtc gcctcatgat atgtctcctg ataaaccatt actccatcga
    13321 ggccaagctg ttttaactcg gcatattccg cctccgccag cggttgcact tccatctgta
    13381 gtgaagagaa ctgttcacgc agggcaggga gatgacgacg aaagtaatcc atccccactt
    13441 tcgcctgatg ttcaccagtg actaatagca gatgttcaaa gcccatctcc cgtatagcgg
    13501 cgctttctct ggcaatatcc gcttcatcca gcgttttgcg cttgatgcgg ttgctcatag
    13561 aaaatccgca ataagtacag tcgttagcgc aaagattgga aagataaagc gggacgtaga
    13621 agctgacgac gttgccaaat cgctgacgag tcagacgttg cgctcgctgg gccagtggtt
    13681 ccagatagcc actggcggcg ggtgatagca atgccatcat gtcgtcgcgg gtgagttgcg
    13741 aggcatttag cgcccgctct acgtcagcag ccgttttgcc gttgatacgc aggcggatgt
    13801 catcccagtc cagttgtcgc cagcgatcgc tgaaggtttt cattccgatg cctccagaaa
    13861 tccagtcagc gggctggtgg catgagcaaa atgactgcgg ctgcccggtc cggactgacg
    13921 tgccagcagg cctgcttcta ccgccagacg aaatgccttc gccatgttga cgggatcgtc
    13981 cgcgacggca atcgccgtat tcactaacac cgcgtcggcc cccatttcca gcgcctgcgc
    14041 ggcatggctg ggaacgccga tgccagcatc gacaaccacc ggaacggtgg cctgctggat
    14101 aataatctcc agcatggcgc gggtttccag tccctgattc gagccaatcg gcgcgccgag
    14161 cggcatcacc gctgcacagc cgacttcttc cagacgttta cacaataccg gatcggcccc
    14221 gcagtaaggc agcacgacaa atccctgttg taccagcttt tcggcggctt tcagggtttc
    14281 gatgggatcg ggcaacagcc agcgggcgtc agggtgaatc tctaatttta accagtttgt
    14341 gcctaacgct tcacgagcca gatgggcggc gaaaatggct tcttccgctg ttttcgcccc
    14401 ggatgtattt ggcagcaggg tcacacccgc cgcgataagc ggttcgagga tagcgtcgtt
    14461 gtgctggcgc aagttgacgc gtttcatcgc cagtgtcacc agttggctgc cgcaagcgcg
    14521 gatcgcctcc atcatcagtt gtgaagaggc gaatttccct gtgccggtaa acagatgtga
    14581 atcaaacgtt ttgtccgcaa tacgtaacat ctcaaccccc tgcaataacc tgaaaaagca
    14641 ggatctggtc gccatcctgc acgatatgtt gcgcccactg ctcacgcggg acgatttgct
    14701 gattaatcgc cagtgccgcg cccgcttgtt gttggtcgag ttgctccagt agttcgtgaa
    14761 ccgtttgccc ggcggcgcac tgcatcggtt gatcgttaaa caggatctgc attgcttcct
    14821 ccgcataccg ggcaaccact ggcgcggcgc aaagccaggc tgcgccactg gctcgactta
    14881 ccgtcgaaca gtcggagttc tcccgcaggc gtctctatac cacttaataa cttaatcgct
    14941 tccagtgcct gtaatgtgcc cataaccccg accaccgggc caaccacgcc cgccgtgcgg
    15001 cagttgcgtt ctggctcctg gttatctggc cacaggcagc ggtaacaccc ctgctcccag
    15061 ggcggcgtca gtaccatcaa ctgaccgcca aatctgaccg cgctggcggt gataagcggc
    15121 gtgttgagtg ccacgcaggt ggcattaatc tcctggcgag tcgccatatt gtcggtacag
    15181 tcgagcacca catcggcccg tgctaccgca tcttttaacg cctcacccgt caaccgttgt
    15241 tgtaatgcca tcagttgaat gtcgggattc aactgtgtca gtcgctgttg gctgacctgt
    15301 gatttcgggc gatcgatatc ttcagtggta aagaggattt gtcgttgcag attgcttaaa
    15361 tgcacatcgt cgtcatctgc cagtaccagc gtcccgacgc cagcccccgc caggtatagc
    15421 gcagcaggtg tacccagccc gccaagacca ataatcagca cctggctgtc gagcagtttt
    15481 tgctgcccgt ccagagcgat atcgtcgagc aggatttggc ggctataacg cataaagtca
    15541 cggtcattca tcgccaactc ctgcaatttc cagcagctgt gccgttgcca aacgccagtc
    15601 tgcggcttga gtaatggcgc tgacgacggc gatactgccg acacccgttg ctatcaccgc
    15661 aggcgcgcgc gccagactga taccgccaat cgccacggtg ggataatccg ctaggcgctc
    15721 aacatgccgt gccagctgtt ccagtccctg cggtgcagaa ggcatctgtt tggtttgtgt
    15781 cggaaacaca tgtcccagcg cgatataaga aggacgtgct gccagcgcga cgtcgatctc
    15841 catatcgtca tgggtcgaaa cgcccagccg caggcctgcc gcgcggatgg cgctgagatc
    15901 ggtggcttgc aaatcttcct gccccagatg gacgccatac gcctgatgct taatcgccag
    15961 ccgccagtaa tcgttgataa acaatcgcgc gttatagcgg cggcccagcg caattgccgc
    16021 caccacatcg gcttctacct cttcatcgcg ctgatctttg atgcgtagct ggagagtacg
    16081 tacgcctgca tccaacagac gttcgatcca ctgtacgctg tcaaccaccg ggtacagtcc
    16141 taaacgaaag ggtacaggag gaaactcagg ctgatacatc acgcttcctc cttacgcagg
    16201 tagatttctc cgcctctggc gcggaagttc tccgacatat ccgccattcc cacttcaatg
    16261 gtttgcgcgg cggcgtaatc acgcacttcc tggctgattt tcatcgagca gaatttcggt
    16321 ccacacatgg agcaaaaatg ggcgacttta ccggactctt gcggcagggt ttcatcgtga
    16381 taagcgcggg cagtaaacgg gtcgagggcc agattaaact ggtcttccca gcgaaattcg
    16441 aagcgggctt tcgacatggc gttatcgcga atttgcgcgc ccggatgccc tttcgccagg
    16501 tcggcggcgt gagcggcaat cttataggtg ataatccctt gtttaacatc ttctttattt
    16561 ggcaggccca gatgctcttt cggcgttacg tagcagagca tcgcgcagcc aaaccagcca
    16621 atcatcgccg cgccaatccc tgacgtgaag tggtcatagc ccggcgcgat atcggtagtt
    16681 agcggcccca gagtgtaaaa cggcgcttcg tggcagtgct ctaactcctc ggtcatattg
    16741 cggcgaatca tctgcatcgg cacgtggcct gggccttcaa tcatcacctg cacgtcatat
    16801 tcccaggcga ttttagtcag ttcacccagc gtatgcagct cggcaaactg tgcttcatcg
    16861 ttggcgtcct gaacagaacc ggggcgcagg ccgtcgccca gcgatagaga aacgtcataa
    16921 gcagcacaga tttcacaaat ttcgcggaag tgttgataga ggaaattttc ctgatgatgg
    16981 gagaggcacc atttcgccat aatcgaaccg ccgcgagaga cgataccggt caggcgtttc
    17041 gcggtcatcg gcacgtagcg cagcagcacc cccgcatgga tagtgaagta atccacacct
    17101 tgctcggcct gttccagcag ggtgtcgcgg aacgcttccc aggtaagatc ttcggcgatc
    17161 ccgttcacct tctccagcgc ctggtagatc ggcactgtac cgatcggcac cgggctgtta
    17221 cgcaaaatcc actcgcgggt ttcgtgaata tagcgaccag tggaaagatc catcacggta
    17281 tccgctcccc agcgcgtgga ccataccagt ttttccactt cttcttcgat ggaagaagtg
    17341 accgccgagt tgccgatatt ggcgttaact tttaccagga agttacggcc aataatcatc
    17401 ggctccgatt ccggatgatt aatgttggcc gggataatcg cacgtccggc agcaacttca
    17461 tcacggacaa attccgcagt gatattttcc ggcagacgtg cgccaaagct cattcccgga
    17521 tgctggtggc gtaaaacttc gctacgtatg cgctcgcgac ccatattctc gcgaatggcg
    17581 atgaattcca tttctggcgt gatgataccc ttgcgggcgt agtgcagttg ggtgacgcgg
    17641 cgtcctgctt tggcgcgttt tggcgttaat acgccgctaa aacgcagctc gtcgagacca
    17701 tcatctgcca gccgcgcttt tgtgtaatcg gaactgcgca cggtaagttc ttcggtatcg
    17761 ccgcgcgcat cgatccacgg ctggcgtagt tttgccagcc cttgctgcac gttaatggcg
    17821 atctgcggat cgccatacgg accggaggtg tcgtagaccg gaatcgcttc gttttcttcg
    17881 tactgcggct gttctttgct gccgccgatt agcgtcgggc taagctggat ctcacgcatt
    17941 ggcacgcgca cgccgggttg tgtgccagtg atgtaaatgc gttttgagtt gggaaaggcg
    18001 gagccttcca gggtgtcgat aaaatgttgg gcctgggcgc gttgttcgcg gcgggtcagt
    18061 tttgttgcag acatagctca ttccaaaaag ttaaggacgt ggcttgtcag acgacggatg
    18121 aagcaagaga cgatcgccgc aaaggcgatg cgatagcaga ttaactcttg ttcccttcgc
    18181 aggtattagc ctgatcaggt tccgcggatc ccgaataaac ggtctcagcc agttaaggca
    18241 ctccgacaag aaattagccc cacaaacggg gcgttgaatg taaattacgc gttaacagcg
    18301 cagaactcaa gctggatatt tgacgcgggc ggcgtcaagc accagtagaa tcagcttatc
    18361 ttccagcaca aagcgcgctt ccagtgcctc gccgatgtca gataaaacct gttgaaactc
    18421 aaggtaatta tcatgatcga tggcggtttc cagactggaa tcgtagtcat ccataatctg
    18481 ttgagtattg gcttcgagtt gtggccaaat cttcgcggcg cgtgcgagtt gaccattgcc
    18541 ttccagctta tgaagaatgc gctcataaat actgaaatgt ccggcagaca agtaatcgac
    18601 caggctctga caaaaatcat caagggcttt ttcgtttagc ctcatgtacg attctttgcc
    18661 aggcttaacg ccaaccagat tgtagtaagc cacgagcaga tgcttacgta catgtagcca
    18721 gcgatcaacc agtttgttac ttcctctgac gcgctccgtc aggttatcga gctggttaag
    18781 catgattgac tccgcaagtt tgtattcaaa aactgctctg tgagaaatgt aaaaaccatg
    18841 ttaaacatgc cagtgatgca aaggtagtgc aagagctatg gatcgtataa ttgaaaaatt
    18901 agatcacggc tggtgggtcg tcagccatga acaaaaatta tggttgccga agggagaatt
    18961 gccatatggc gaagcggcaa atttcgatct tgtgggtcag cgcgcactac aaatcggcga
    19021 atggcagggg gaacctgttt ggttagtaca actgcagcgg cgtcacgata tggggtcggt
    19081 acgtcaggtc attgatctcg atgttgggct gtttcaactg gccggacgtg gcgtgcaact
    19141 ggcggagttt taccgatcgc ataaatactg tggttactgc gggcatgaaa tgtatccaag
    19201 caaaaccgaa tgggcgatgc tgtgcagcca ttgccgtgag cgttactacc cgcaaatcgc
    19261 cccctgcatt attgttgcca tccgtcgcga tgattcgatc ctcctcgccc agcatacccg
    19321 ccatcgtaac ggtgtccata cagtacttgc cggattcgtc gaagtgggtg aaaccctcga
    19381 gcaggcagtc gcgcgggaag tgatggaaga gagcggaatt aaagttaaaa acttgcgtta
    19441 cgtgacctcc caaccgtggc cgtttcctca gtcgttaatg accgcgttta tggcggaata
    19501 tgacagcggc gagatcgtga tcgacccgaa agaattgctt gaggcgaact ggtatcgcta
    19561 tgacgatttg ccgttactcc cgccgcccgg caccgtagcg cgccgtctga tagaagatac
    19621 agtggcgatg tgtcgggcag agtatgagtg atgatacact gaccgcctga cgcactaagg
    19681 aacagccaaa atgaccgaac ttaaaaacga tcgttatctg cgggcgctgc tgcgccagcc
    19741 cgttgatgtc actccagtat ggatgatgcg ccaggcgggt cgctatctac cggaatataa
    19801 agccacgcgc gcccaggcgg gcgattttat gtcgctgtgc aaaaatgccg agctggcgtg
    19861 cgaagtgact ttgcaaccgc tgcgtcgcta tccgctggat gcggcgatcc tcttttccga
    19921 tatcctcacc gtgccggacg cgatggggtt agggctctat tttgaagccg gagaaggtcc
    19981 gcgttttacc tcgccagtca cctgcaaagc cgacgtcgat aaactgccaa ttccggaccc
    20041 ggaagatgag ctgggttacg tgatgaacgc ggtgcgtacc attcgtcgcg aactgaaagg
    20101 cgaagtgccg ctgattggtt tttccggcag cccgtggacg ctggcgactt acatggttga
    20161 aggcggtagc agcaaagcct tcaccgtgat taaaaagatg atgtatgccg atccgcaggc
    20221 gctgcacgcg ctgctcgata aactggcgaa aagcgtcacg ttgtatctga atgcgcagat
    20281 taaagccggt gctcaggcag tcatgatttt cgacacctgg ggcggcgtgc ttaccgggcg
    20341 cgattatcaa cagttctcgc tttattacat gcataaaatt gttgatggtt tactgcgtga
    20401 aaacgacggt cgccgcgtgc cagtcacgct gtttaccaaa ggtggcggac agtggctgga
    20461 agcgatggca gaaaccggtt gcgatgcgct gggcctcgac tggacaacgg acatcgccga
    20521 tgcgcgccgt cgcgtgggca ataaagtcgc gctgcagggt aatatggacc cgtcgatgct
    20581 gtacgcgccg cctgcccgca ttgaagaaga agtggcgtct atacttgccg gtttcggtca
    20641 cggcgaaggc catgtcttta accttggtca cggcattcat caggatgtgc cgccagaaca
    20701 tgcaggcgtg tttgtggagg cagtgcatcg cttgtctgaa cagtatcacc gctaaggagc
    20761 gattatggat ctcgcgtcat tacgcgctca acaaattgaa ctggcttctt ctgtgatccg
    20821 cgaggatcga ctcgataaag atccgccgga tctgatcgcc ggagccgatg tcggatttga
    20881 gcagggcgga gaagtgacgc gagcggcgat ggtgctgctg aaatacccct cgcttgagct
    20941 ggtggagtat aaagtggccc gcatcgccac caccatgcct tacattccag gttttctctc
    21001 gttccgcgaa tatcctgcgc tgctggcagc gtgggagatg ctgtcgcaaa agccggattt
    21061 agtgtttgtc gatggtcatg ggatctcgca tcctcgccgt cttggcgtag ccagccattt
    21121 tggcttattg gtggatgtgc cgaccattgg cgtagcgaaa aaacggctct gcggtaaatt
    21181 cgaaccgctc tccagcgaac cgggcgcgct ggccccgctg atggataaag gcgagcagct
    21241 ggcctgggtc tggcgcagca aagcgcgctg taacccgttg tttatcgcta ccggccatcg
    21301 ggtcagcgtg gacagcgcgc tggcgtgggt acaacgctgc atgaaaggct atcgtctgcc
    21361 ggagccaacg cgctgggcgg acgcggtggc ctcggaacgt ccggcgttcg tgcgctatac
    21421 agcaaatcag ccctaattca ggtaaactgc ggataatttc cgtatttgag aactcaacat
    21481 gttacaaaac ccaattcatc tgcgtctgga gcgcctggaa agctggcagc atgtcacttt
    21541 tatggcctgc ttatgcgaac gcatgtaccc caattacgcc gtattctgtc agcaaaccgg
    21601 cttcggcgat gggcagattt accgccgcat tctcgatctc atttgggaaa cgctgaccgt
    21661 caaagacgca aaagtaaatt tcgacagcca actggagaaa tttgaagaag cgattccttc
    21721 agccgacgat ttcgatctgt acggcgttta tccggcaatt gatgcttgcg tggcgttaag
    21781 tgaactggtc cattcgcgtt taagtggcga aacgctcgaa cacgcggtgg aagtgagtaa
    21841 gacctccatc acgaccgtgg cgatgctgga aatgactcag gctggtcgcg aaatgagcga
    21901 tgaagagctc aaagaaaacc cagctgtaga gcaagaatgg gacattcagt gggaaatatt
    21961 ccgactttta gctgagtgcg aagaacgcga tatcgagctg ataaaaggcc ttagggcaga
    22021 cctgcgtgag gcgggtgaga gcaatattgg tataattttt cagcaataag accagaaaac
    22081 gtgatttaac gcctgatttg tcgtacctgg agtcttccct ttcgcccccc gtctggtcta
    22141 catttggggg gcgaaaaaaa gtggctatcg gtgcgtgtat gcaggagagt gctattctgg
    22201 catttccgtc gcactcgatg cttagcaagc gataaacaca ttgtaaggat aacttatgaa
    22261 caagactcaa ctgattgatg taattgcaga gaaagcagaa ctgtccaaaa cccaggctaa
    22321 agctgctctg gagtccactc tggctgcaat tactgagtct ctgaaagaag gcgatgctgt
    22381 acaactggtt ggtttcggta ccttcaaagt gaaccaccgc gctgagcgta ctggccgcaa
    22441 cccgcagacc ggtaaagaaa tcaaaattgc cgcagctaac gtaccggcat ttgtttctgg
    22501 caaggcactg aaagacgcag ttaagtaaga ttgcgtggca gtgaacagtt ttaacgaagg
    22561 ggtggtttca ccccttttgt ctttctggcg tcgatcattg atgctggcag gcgctctgtt
    22621 tctcactgcc tgtagtcaca actcttcact tcctcccttt accgccagtg gatttgctga
    22681 agaccagggc gcggtacgca tctggcgaaa agacagcggc gataatgtgc atctgcttgc
    22741 cgtgtttagc ccgtggcgca gtggcgatac cacgacgcga gagtatcgct ggcagggcga
    22801 taaccttacg ctcatcaata tcaatgttta cagcaaaccg ccggtgaaca ttcgcgcgcg
    22861 ttttgacgat cgcggcgatc tgagctttat gcaacgtgaa tccgatgggg aaaagcagca
    22921 gctttctaac gaccaaatcg atttataccg ttatcgtgct gatcagatcc gccagattag
    22981 cgatgcctta cgtcagggga gagtcgtgct gcgccagggg cgctggcatg cgatggaaca
    23041 gaccgtgacc acctgcgaag ggcaaaccat taaacctgat ttagattcgc aggcgatagc
    23101 gcatatcgag cgccgccaga gccgctcttc tgttgatgtc agcgtggcat ggctggaagc
    23161 gcccgaaggt tcgcaattac tgttggtggc aaactctgat ttctgtcgct ggcaacccaa
    23221 cgagaaaacg ttctgattta ccagtggccc atacccatat gaccgccacc accgcagtcg
    23281 ccgtaaccca ttccggtacc gcgcggaata cccgcttcag ccatcgcaat atctcgtttc
    23341 acccgtaact catctaacga ctgacgcaaa ttctccatct ctttggcgac cgcgttaatt
    23401 ttgctgctat ccggtgggtt cgcggctaac agggcattgt attcataacg ctttgtcacc
    23461 agttgctgtt gcagtgcgct gctttgagcg taaaagtcat tatggatttt ctgccacgct
    23521 gtctgttgtt cgctggtcaa aggcgcggca ttttgctgcc acataccatg tccgccgtga
    23581 gcaaatgcag atgtcgatcc catcgccatt gctgaaagcg ccatcattac cagggcaatt
    23641 ttcgtgttcc gtttcatggt taatcctcca gtggttgtct tacttcgggt attgcatctt
    23701 ccgtgccaac ggcgaaacgc tgatatgacg ggtaatctgg catgataaac gagtaaaaat
    23761 gactcgcctg ctgcgggtag cgagtcattt ttactcattg aaacttaagc ctttgtgtta
    23821 cagctcaggg taagcgctga taaaagatgg catgatttct gctgtcagaa agggatggac
    23881 aggcaaagaa gaagatgcgt tttatgcaac gttctaaaga ctccttagct aaatggttaa
    23941 gcgcgatcct ccccgtggtc attgttgggc tggtggggct gtttgcggtg acagtgattc
    24001 gcgattatgg gcgcgagact gccgccgcca gacaaacgct gctggaaaaa ggcagtgtac
    24061 ttattcgtgc tcttgaatcc ggctcgcgcg tcggcatggg gatgcgcatg catcatgcgc
    24121 agcagcaggc cttacttgaa gaaatggccg ggcagcctgg agtacgttgg tttgcggtca
    24181 cggatgaaca aggaacaatc gtgatgcata gcaactccgg catggtggga aaacagcttt
    24241 attccccgca ggaaatgcag cagttacatc cgggaaatga agaagcgtgg cggcggatcg
    24301 atagcgcaga cggcgagcct gttctggaaa tttatcgcca gtttcaaccg atgtttgcta
    24361 ctggaatgca ccggatgcgc catatgcagc agtatgccgc gacaccacaa gcaattttta
    24421 tcgctttcga cgccagtaac attgtgagtg ccgaagatcg tgagcagaga aacaccctga
    24481 ttatcctctt cgccctggcg acggtcttgc tggcaagcgt gttgtcattc ttctggtatc
    24541 gccgatatct gcgctcgcgg cagctattgc aggatgaaat gaagcgcaaa gagaagctgg
    24601 tggcactggg gcatctggcg gcaggcgttg cccacgaaat ccgtaatcca ctttcctcga
    24661 ttaaagggct ggcgaaatac tttgccgaac gcgcgtcagc agggggagaa gcgcatcaat
    24721 tggcgcaggt gatggcgaaa gaagccgacc gtttaaaccg cgtggtaagc gagctgctgg
    24781 aactggttaa gccaacgcat ctggctttgc aggcggtgga gctcaacacg ctgattagcc
    24841 actcattaca gctggtaagc caggatgcaa acagccggga gatccagtta cgctttaccg
    24901 ccaacgacac attaccggaa attcaggccg atccggacag gctgactcag gtcctgttga
    24961 atctctatct caacgctatt caggcgattg gtcagcatgg cgtgattagc gtgacggcca
    25021 gcgaaagcgg cgcgggcgtg aaaatcagcg ttaccgacag cggtaaggga attgcggcgg
    25081 atcagcttga agccatcttc actccgtact tcaccaccaa agccgaaggc accggactgg
    25141 ggctggcggt tgtgcataat attgttgaac aacacggtgg tacaattcag gtcgcaagcc
    25201 aggagggaaa aggcgcaacg ttcacccttt ggcttccggt caatattacg cgtaaggacc
    25261 cacaaggatg acgcacgata atatcgatat tctggtggtg gatgatgaca ttagccactg
    25321 cactattttg caggctttgc tgcgcggctg gggctataac gtcgcgctgg cgaacagcgg
    25381 gcgacaggcg ctggagcagg tgcgggaaca ggtttttgat cttgtgcttt gcgatgtgcg
    25441 aatggcagag atggatggta tcgccacgct aaaagagatc aaaacattaa accccgcgat
    25501 tccggtgctg attatgaccg cgtactccag cgtcgagacg gcggtagagg cgttgaaaac
    25561 cggggcgctg gattatctca tcaagccgct ggatttcgat aacctacagg cgacgctgga
    25621 aaaggcgctc gcgcatacac acagtgttga cgctgaaacg cctgcggtgt ctgccagcca
    25681 gttcggtatg gtcggtaaaa gcccggcgat gcaacacctg ctcagtgaaa tcgccctcgt
    25741 cgcgccatcg gaagccacgg tgctgatcca cggcgattcc ggcaccggta aagagctggt
    25801 cgccagggcg attcacgcca gtagcgcacg tagcgaaaaa ccgctggtaa cgctcaactg
    25861 cgcggcgctc aacgaatcct tgctggagtc tgaattgttc ggtcacgaaa aaggggcgtt
    25921 taccggggcc gacaaacgcc gggaagggcg ctttgttgag gcggacggcg gcacgttgtt
    25981 tctcgatgaa attggcgata tctcgccgat gatgcaggtg cgtctgctac gtgcgattca
    26041 ggagcgcgaa gttcagcgtg tcggtagcaa ccagactatt tcggttgatg tccggctgat
    26101 tgcggcgacc catcgcgatc ttgccgcaga ggtgaatgcc gggcgttttc gccaggatct
    26161 ctactatcgg ctgaacgtgg tggcgattga aataccatcg ctgcggcaac ggcgggaaga
    26221 tattcctctg ctggctggcc attttcttca gcgctttgcc gaacgtaatc gcaaggcggt
    26281 aaaaggcttt acgccccagg cgatggatct gctgattcat tacgactggc cgggaaatat
    26341 tcgtgaactg gaaaacgcgg tggaacgggc ggtggtgctg ctgaccgggg aatatatttc
    26401 cgaacgcgag ctgccgctgg cgattgccag tacgccgatc ccgctggtac aaagtcagga
    26461 tattcagccg ctggtggaag tcgaaaaaga agtgattctg gcagcactgg agaaaacggg
    26521 cggcaacaaa accgaagccg cccgtcagtt agggatcacg cgtaaaacgc tattggcaaa
    26581 actgtcgcgt tagttctgct cgcgttcgat agcgcgccag ccgatatctt tccggcagaa
    26641 gcagtcgtcc caatggatat cggtcattaa ggcataagcg cgtttctgtg cttctgccac
    26701 ggtatgaccc agcgcggtaa cgcacagtac gcgcccgccg ctggtcacta cctgctcgtc
    26761 atccgccagt ttcgtgcccg cgtggaacac tttgccgtct gccacttctt ccagcggcag
    26821 gccgtggatc acatcgccgg tgcggtaatc gcccggatat ccacccgcag ccatcaccac
    26881 gccgagagaa gcgcgttcat cccactcgga cgttttctcg tccagcttgc cttcacaggc
    26941 cgccaggcag agctcaacca gatcggactt catgcgcagc ataatcggct gggtttccgg
    27001 atcgccaaag cggcagttaa attcaataac cttcggattg ccctgtttgt cgatcatcag
    27061 gcccgcgtag agaaaaccag tgtaggtgtt gccttccgcc gccatgcctt tcacggttgg
    27121 ccagatgata cgttccatcg tgcgctgatg aacctcatcg gtcactactg gcgcagggga
    27181 gtaagcgccc atcccgccgg tgttcggccc ggtatcttta tcgcctacgc gtttgtgatc
    27241 ctggctggtg gccatcggca gcacatgctc gccgtccacc atcacgataa agctcgcttc
    27301 ttcgccgtcg aggaactctt cgataacaat gcgatgaccc gcgtcgccaa acgcgttgcc
    27361 cgccagcatg tcgcgcacgg cggcttcagc ttcttccagc gtcatcgcca cgataacgcc
    27421 tttcccggcg gccagaccgt ccgctttaat gacgattggc gcgcctttct cacgcagata
    27481 cgccagtgca ggttctacct cggtgaagtt ctggtattcc gctgtgggaa tgttatggcg
    27541 ggccagaaag tctttggtga atgctttaga accttccagt tgtgcggcac ctgcggttgg
    27601 gccgaagatt ttcagcccgg cggcgcggaa ggtatcgact acgcctttca ccagcggcgc
    27661 ttccgggccg acgatggtta gatctacctt ttcgttttgt gcgaaatcca gcagcgccgg
    27721 gatatcggtt acgccgatag cgacgttctg tagcgcgggt tccagcgcag tgcctgcatt
    27781 acccggagca acaaaaacag tttcaaccag cggcgactgg gccgctttcc aggccagcgc
    27841 gtgctcgcgc ccgccgttac cgatcactaa tactttcatc tgtcgctcca ttaatggcgg
    27901 aagtggcgca tgtcggtgaa gagcatcgca ataccgtgct cgtcggcggc ggcaattact
    27961 tcgtcatcac ggatagaacc gccaggctgg attacgcagg tcacgcccgc agcggctgcg
    28021 gcatcaatac cgtcgcggaa cgggaagaac gcgtcagaag ccatcgagga acctttcact
    28081 tccagacctt catcggcagc tttgataccg gcgattttcg cggagtacac gcggctcatc
    28141 tggcctgcgc caataccgat ggtcatattg tttttggcat agacgatagc gttggatttc
    28201 acgaacttcg ccactttcca gcagaacagc gcatcacgaa gttcctgttc agtcggctga
    28261 cgtttggtga cgacgcgcag ttcttccgca ccgaccatcc ccagatcgcg atcctgaacc
    28321 agcagaccgc cgttcacgcg tttgaaatca agacccggaa tacgttcgcc ccactgaccg
    28381 caggtcagaa cgcgtacgtt ctgcttggcg gcggtgattt tcagggcttc ttcgctggcg
    28441 gacggcgcaa taatcacttc gacgaactga cgagaaatga tggcctgcgc ggtttcagca
    28501 tccagttcgc ggttaaaggc aatgatgccg ccgaatgcgg aggttgggtc ggttttgtac
    28561 gcgcgatcgt aagcatcaag aatggaattg ccgatagcca cgccgcaagg gttggcgtgt
    28621 ttcacaatca cacatgccgg ctcggcgaac tctttcacgc actccagcgc cgcatcggta
    28681 tcggcgatgt tgttataaga gagggcttta ccctgaacct gggttgcggt ggcaacggag
    28741 gcttctttca cattctcttc tatatagaag gcagcctgct ggtggctgtt ttcgccgtaa
    28801 cgcatatcct gcttcttaat gaagttcagg ttcagcgtgc gtgggaagcg accggcggct
    28861 tctttgtttt caccgtggta agccggaacc atgctgccga agtagttggc aatcatgctg
    28921 tcgtaggcgg cggtgtgttc gaaggctttg atggcaaggt caaagcgagt ttccagagtc
    28981 agggagcctt cattggcatc tatctcttta ataatggcgt cgtagtcgct gctcttcacc
    29041 acgatggcga catctttatg gttcttggcg gcagagcgca ccatcgttgg gccgccgata
    29101 tcgatgttct caaccgcatc ttccagcgag caaccttcac gggcaacggt ctgggcgaac
    29161 ggatacagat taacaaccac catatcgata ggctggatct gatgttcttc cataatggca
    29221 tcgtcctggt cgcgacggcc cagaatgcca ccatgtactt tcggatgcag ggtcttcacg
    29281 cgtccatcca tcatctccgg gaaaccggtg taatcggaaa cttcggttac cggcagacct
    29341 ttttctgcta acagacgggc tgtgccccct gtagacagca gctccacacc gcgtgcggaa
    29401 agtgcctggg cgaattcgac gataccggct ttgtcagaaa cactgagcag agcgcggcgg
    29461 actggacgac gttgttgcat ggtaaatccc ctggatttga ctattacaga gagcgttagc
    29521 tgaatttttc gcgaaaaact cagctaacgc ccctaacggg gcatccttat ttttcgcccg
    29581 cattgtaacg aaaacgtttg cgcaacgctc gcgaattttt ctctttcaat ggtgatcaca
    29641 attttgacag tggctaccgt gggaaaaata cagaaattac attgaagatt gtggataact
    29701 ctgtgcgtaa aaaggtataa agcggggttt tgctggggaa tgcagcagtc agtcattttt
    29761 atgcaatttt tctattgcgg cctgcggaga actccctata atgcgcctcc atcgacacgg
    29821 cggatgtgaa tcacttcaca caaacagccg gttcggttga agagaaaaat tctgaa
//
LOCUS       DAZFFE010000050        29547 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFFE010000050 DAZFFE010000000
VERSION     DAZFFE010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.050"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(26..872)
                     /locus_tag="ACHJ69_002451"
                     /pseudo
     CDS             complement(26..872)
                     /locus_tag="ACHJ69_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000044502.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1000..1175)
                     /locus_tag="ACHJ69_002452"
                     /pseudo
     CDS             complement(1000..1175)
                     /locus_tag="ACHJ69_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1387..2232)
                     /gene="araC"
                     /locus_tag="ACHJ69_002453"
     CDS             complement(1387..2232)
                     /gene="araC"
                     /locus_tag="ACHJ69_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO5015416.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAHEWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCE"
     gene            2560..4260
                     /gene="araB"
                     /locus_tag="ACHJ69_002454"
     CDS             2560..4260
                     /gene="araB"
                     /locus_tag="ACHJ69_002454"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO5015417.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQNGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCYTPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDNAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRILSWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            4271..5773
                     /gene="araA"
                     /locus_tag="ACHJ69_002455"
     CDS             4271..5773
                     /gene="araA"
                     /locus_tag="ACHJ69_002455"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO5015418.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            6058..6753
                     /gene="araD"
                     /locus_tag="ACHJ69_002456"
     CDS             6058..6753
                     /gene="araD"
                     /locus_tag="ACHJ69_002456"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO5015419.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRGRGVLVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGINA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            6828..9179
                     /gene="polB"
                     /locus_tag="ACHJ69_002457"
     CDS             6828..9179
                     /gene="polB"
                     /locus_tag="ACHJ69_002457"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO5015420.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPLLLEKLNAWFATHDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNDVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKTLIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RVLVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFCNEPYHEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENHKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            9344..12250
                     /gene="rapA"
                     /locus_tag="ACHJ69_002458"
     CDS             9344..12250
                     /gene="rapA"
                     /locus_tag="ACHJ69_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO5015421.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQQGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            12262..12921
                     /gene="rluA"
                     /locus_tag="ACHJ69_002459"
     CDS             12262..12921
                     /gene="rluA"
                     /locus_tag="ACHJ69_002459"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO5015422.1"
                     /translation="MGMENYNPPQEPWLVVLYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(13039..13854)
                     /gene="djlA"
                     /locus_tag="ACHJ69_002460"
     CDS             complement(13039..13854)
                     /gene="djlA"
                     /locus_tag="ACHJ69_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO5015423.1"
                     /translation="MQYWGKIIGVTVALIMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            14109..16463
                     /gene="lptD"
                     /locus_tag="ACHJ69_002461"
     CDS             14109..16463
                     /gene="lptD"
                     /locus_tag="ACHJ69_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO5015424.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYKDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTRLAESANRVMPQFKVDGRMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSKIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISDRWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            16516..17802
                     /gene="surA"
                     /locus_tag="ACHJ69_002462"
     CDS             16516..17802
                     /gene="surA"
                     /locus_tag="ACHJ69_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO5015425.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            17802..18791
                     /gene="pdxA"
                     /locus_tag="ACHJ69_002463"
     CDS             17802..18791
                     /gene="pdxA"
                     /locus_tag="ACHJ69_002463"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO5015426.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLIVQLAQREWPVEQVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTVGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELREQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            18788..19609
                     /gene="rsmA"
                     /locus_tag="ACHJ69_002464"
     CDS             18788..19609
                     /gene="rsmA"
                     /locus_tag="ACHJ69_002464"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO5015427.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAA
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            19612..19989
                     /gene="apaG"
                     /locus_tag="ACHJ69_002465"
     CDS             19612..19989
                     /gene="apaG"
                     /locus_tag="ACHJ69_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO5015428.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            19996..20844
                     /gene="apaH"
                     /locus_tag="ACHJ69_002466"
     CDS             19996..20844
                     /gene="apaH"
                     /locus_tag="ACHJ69_002466"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO5015429.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYNIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDMGE
                     GEAVAS"
     gene            complement(20841..21155)
                     /locus_tag="ACHJ69_002467"
     CDS             complement(20841..21155)
                     /locus_tag="ACHJ69_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HFO5015430.1"
                     /translation="MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(21158..21391)
                     /locus_tag="ACHJ69_002468"
     CDS             complement(21158..21391)
                     /locus_tag="ACHJ69_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO5015431.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(21477..21956)
                     /gene="folA"
                     /locus_tag="ACHJ69_002469"
     CDS             complement(21477..21956)
                     /gene="folA"
                     /locus_tag="ACHJ69_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO5015432.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(22148..24010)
                     /gene="kefC"
                     /locus_tag="ACHJ69_002470"
     CDS             complement(22148..24010)
                     /gene="kefC"
                     /locus_tag="ACHJ69_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO5015433.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFVLSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLEKPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHGPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(24003..24533)
                     /gene="kefF"
                     /locus_tag="ACHJ69_002471"
     CDS             complement(24003..24533)
                     /gene="kefF"
                     /locus_tag="ACHJ69_002471"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO5015434.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(24641..25972)
                     /gene="yaaU"
                     /locus_tag="ACHJ69_002472"
     CDS             complement(24641..25972)
                     /gene="yaaU"
                     /locus_tag="ACHJ69_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5015435.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFEEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(26031..26318)
                     /gene="fixX"
                     /locus_tag="ACHJ69_002473"
     CDS             complement(26031..26318)
                     /gene="fixX"
                     /locus_tag="ACHJ69_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO5015436.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(26315..27601)
                     /gene="fixC"
                     /locus_tag="ACHJ69_002474"
     CDS             complement(26315..27601)
                     /gene="fixC"
                     /locus_tag="ACHJ69_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5015437.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(27652..28593)
                     /gene="fixB"
                     /locus_tag="ACHJ69_002475"
     CDS             complement(27652..28593)
                     /gene="fixB"
                     /locus_tag="ACHJ69_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO5015438.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLSGKPDERMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LNAAVSNDASAVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDVAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(28608..29378)
                     /gene="fixA"
                     /locus_tag="ACHJ69_002476"
     CDS             complement(28608..29378)
                     /gene="fixA"
                     /locus_tag="ACHJ69_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO5015439.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     VLAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         7206 a         8079 c         7568 g         6694 t
ORIGIN      
        1 tataaatata ttttgacatc tctactcacg acctgtgcgg ataatgaggg tttaagaaaa
       61 gaaaaagcgg tctttcaaag ggagattctg aaaactttat agctcccgaa cttgataatg
      121 aactatacaa ctcatgattt attctgtttt tatctttata ataattagta acaggtctat
      181 tattatattg attatataac aatgttgaaa aagactcatc taatggcttt atataattta
      241 accattctgg aagcttcttt tttattagtt gattttttta aaaggaggat gggttgtaaa
      301 taagaatggc gtaaactcag tatttttaaa attcccttgc gctccatatg tatcccatat
      361 ttgtaaggct tttgtgtaag ataaaggttt tgccaataaa agtggatcgg ggcctgttcc
      421 aaatacacga taaaaaccaa tcaagtctga gactaaatct tctgcactaa atccgctatc
      481 agtataccag ttaaaaataa atgagttttg ccatgcgtca aacctcttcg cagttccata
      541 cattaacgtt aaggcaactc ttgctttaag agtgggtgaa agacatgaac gaaccataac
      601 cttcgtataa acaccggtat gaaatgtaag atctactcct aatatttttt ccgaattccc
      661 atttcctgat gataatcaac aggaaaatac caatcatgaa attcatcgta atatgttgaa
      721 aaatgttctt gctccaattt tctttttaat attctggcat cttgtccctg agcatgcccc
      781 aaatcgatcc aacctaactc ttcggtataa actaatccat actttttcgt tgttgcaaat
      841 attccatcag taatatcgtt acgtttgctc atcacaatct cccctcaaaa aaaacgtata
      901 gcaaataata tcaataagga aaaatattat ttttcaatat ttttttgtat acaattcatt
      961 ttatgaatga atttacaaag atagctgcgc ctgatgccgc tacacttttg ccagaccatg
     1021 ttctcctctg agggagtaac tggcgttttt catagaatgc cgtacttttt tcaacaactg
     1081 ccacatcgaa cggcatttat ggtggcgtga agtgcctgcc ataaccgctc aatgtggtgt
     1141 tcctacgatg aacaaaccgc ttgatatatt ctcctgatgc aaccggacaa tatgttcatg
     1201 atgggtatcc cgatacataa gccttcgttt tatttttaca aaaagaaatc gttgtcgata
     1261 ttttatcaag aaaaaaaata attcaggtaa aacgattcaa ctaaaaataa ctaaaattaa
     1321 tcccccgtga aatatataaa taacgtcaga tgaagaaagc aagggggttc ccccccttct
     1381 ttcttattat tcacaacctg ccctaaactc gctcggactc gccccggtgc attttttaaa
     1441 tactcgcgag aaatagagtt gatcgtcaaa accgacattg cgaccgacgg tggcgatagg
     1501 catccgggtg gtgctcaaaa gcagcttcgc ctgactgatg cgctggtcct cgcgccagct
     1561 taatacgcta atccctaact gctggcggaa caaatgcgac agacgcgacg gcgacaggca
     1621 gacatgctgt gcgacgctgg cgatatcaaa attactgtct gccaggtgat cgctgatgta
     1681 ctgacaagcc tcgcgtaccc gattatccat cggcggatga agcgactcgt taatcgcttc
     1741 catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcaattccg aatagcgccc
     1801 ttccccttgt ccggcattaa tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc
     1861 ttcatccggg cgaaagaaac cggtattggc aaatatcgac ggccagttaa gccattcatg
     1921 ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgtgag cctccggatg
     1981 acgaccgtag tgatgaatct ctccaggcgg gaacagcaaa atatcacccg gtcggcagac
     2041 aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat taagaatata
     2101 acccttcatt cccagcggtc gatcgataaa aaaatcgaga taaccgttgg cctcaatcgg
     2161 cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg gatcattttg
     2221 cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt gtccatattg
     2281 catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc aaccggtaac
     2341 cccgcttatt aaaagcattc tgtaacaaag ccatgacaaa aacgcgtaac aaaagtgtct
     2401 ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca
     2461 tagcattttt atccataaga ttagcggatc cagcctgacg ctttttttcg caactctcta
     2521 ctgtttctcc atacccgttt ttttggatgg agtgaaacga tggcgattgc aattggcctc
     2581 gattttggca gtgattctgt gcgagctttg gcggtggact gcgccaccgg tgaagagatc
     2641 gccaccagcg tagagtggta tccccgttgg cagaacgggc aattttgtga tgccccgaat
     2701 aaccagttcc gtcatcatcc gcgtgactac attgagtcaa tggaagcggc gctgaaaact
     2761 gtgcttgcag agcttagcgt cgaacagcgc gcagctgtgg tcgggattgg cgttgacaca
     2821 accggctcga cgcccgcacc gattgacgcc gacggtaacg tgctggcgct gcgaccggag
     2881 tttgccgaga acccgaacgc gatgttcgta ttgtggaaag accacaccgc ggttgaagaa
     2941 gcggaagaga ttacccgctt atgttacacg ccgggcaacg ttgactactc ccgctatatt
     3001 ggcggtattt attccagcga atggttctgg gcaaaaatcc tgcatgtaac tcgccaggac
     3061 aacgccgtgg cacaatctgc cgcatcatgg attgagctgt gcgactgggt gccagctctg
     3121 ctttccggta ccacccgccc gcaggatatt cgtcgcggac gttgcagcgc cgggcataaa
     3181 tctctgtggc acgaaagctg gggtggcctg ccgccagcca gtttctttga tgagctggac
     3241 ccaatcctca atcgccattt gccttccccg ctgttcactg atacctggac tgccgatatt
     3301 ccggtgggca ccttatgccc ggaatgggcg cagcgtctcg gcctgcctga aagcgtggtg
     3361 atttccggcg gcgcgtttga ctgccatatg ggcgcagttg gcgcaggcgc acagcctaac
     3421 gcactggtaa aagttatcgg tacttccacc tgcgacattc tgattgccga caaacagagc
     3481 gttggcgagc gggcagtgaa aggtatttgc ggtcaggttg atggcagcgt ggtgcctgga
     3541 tttatcggtc tggaagcagg ccaatcggca tttggggata tctacgcctg gtttggacgc
     3601 atactcagct ggccgctgga acagcttgcc gcccagcatc cggaactgaa agagcaaatc
     3661 aacgccagcc agaaacaact gcttccggcg ctgaccgaag catgggccaa aaatccgtct
     3721 ctggatcacc tgccggtggt gctcgactgg tttaacggtc gccgcacgcc aaacgctaac
     3781 caacgcctga aaggagtgat taccgatctg aacctcgcca ccgacgctcc gctgctgttc
     3841 ggcggtttaa ttgccgccac cgcctttggc gcacgcgcaa ttatggagtg cttcaccgat
     3901 caggggatcg ccgtcaataa cgtgatggcg ctgggcggca tcgcgcggaa aaaccaggtc
     3961 attatgcagg cctgctgcga cgtgctgaat cgcccgctgc aaattgttgc ctctgaccag
     4021 tgctgtgcgc tcggtgcggc gatttttgct gctgtcgccg cgaaagtgca cgcagacatc
     4081 ccatcagccc agcaaaaaat ggccagtgcg gtagagaaaa ccctgcaacc gcgcagcgaa
     4141 caggcacaac gctttgaaca gctttatcgc cgctatcagc aatgggcgat gagcgccgaa
     4201 caacactatc ttccaacttc cgccccggca caggctgccc aggccgttcc gactctataa
     4261 ggacacgaca atgacgattt ttgataatta tgaagtgtgg tttgtaattg gcagccagca
     4321 tctttacggc ccggagactc tgcgccaggt gacgcaacat gcggaacacg ttgttaatgc
     4381 actgaataca gaagcgaagt tgccctgcaa actggtgctg aaaccgctgg gcaccacgcc
     4441 ggatgaaatc accgctattt gccgcgacgc gaattacgac gatcgttgcg ccgggctggt
     4501 agtgtggctg cacactttct ccccggccaa aatgtggatc aacggcctga ccatgctcaa
     4561 caaaccgttg ctgcaattcc acacccagtt caacgcggcg ctgccgtggg acagcatcga
     4621 catggacttt atgaacctga accagaccgc gcatggcggt cgcgagttcg gcttcatcgg
     4681 cgcgcgtatg cgtcaacaac atgccgtcgt taccggtcac tggcaggata aacaagcaca
     4741 tgagcgtatc ggctcctgga tgcgtcaggc ggtctctaaa caggataccc gtcatctgaa
     4801 agtctgccgt tttggcgata acatgcgtga agtagcggtc accgatggcg ataaagttgc
     4861 cgcacagatc aagttcggtt tctccgtcaa tacctgggcg gttggcgatc tggtgcaggt
     4921 ggtgaactcc atcagcgacg gcgatgttaa cgcgctagtc gatgagtacg aaagctgcta
     4981 caccatgact cctgccacac aaatccacgg cgaaaaacga cagaacgtgc tggaagcggc
     5041 gcgtattgag ctggggatga agcgtttcct ggaacaaggt ggcttccacg ctttcactac
     5101 tacctttgaa gatttgcacg gcctgaaaca gcttccgggt ctggccgtac agcgtctgat
     5161 gcagcagggc tacggctttg cgggcgaagg cgactggaaa actgccgccc tgcttcgcat
     5221 catgaaggtg atgtcaaccg gtctgcaggg cggcacctcc tttatggagg actacaccta
     5281 tcacttcgag aaaggtaatg acctggtact cggctcccat atgctggaag tctgtccgtc
     5341 gatcgctgtg gaagagaaac cgatcctcga cgttcagcat ctcggtattg gcggtaaaga
     5401 cgatcctgcc cgcctgatct tcaacactca aaccggtcca gccattgtcg ccagcctgat
     5461 tgatctcggc gatcgttacc gtctgctggt taactgcatc gacacggtga aaacaccgca
     5521 ctccctgccg aaactgccgg tggcgaatgc gctgtggaaa gcgcaaccgg atctgccgac
     5581 cgcttccgaa gcgtggatcc ttgccggtgg cgcgcaccat accgtcttca gccatgcgct
     5641 gaacctcaac gatatgcgcc agttcgccga gatgcacgac attgaaatca cagtgattga
     5701 taacgatacc cgccttccag cgtttaaaga cgcgctgcgc tggaacgaag tgtattacgg
     5761 atttcgtcgc taagtagccg catcaggtat gtaacgcctg atgcgacgct gacgcgtctt
     5821 atcaggccta cgcgttccgg atttgtaggc cggataagta aagcgcatcc ggcattcacc
     5881 gcctgatgcg gcgctggcgc gtcttatcag gcctacgcgt tccggatttg taggccggat
     5941 aagcaaagcg catccggcat tcaccgcctg atgcgacgcc gacgcgtctt atcaggccta
     6001 cacgctgcga ttttgtaggc cggataagca aagcgcatcc ggcacgaagg agtcaacatg
     6061 ttagaagatc tcaaacgcca ggtattagaa gccaacctgg cgctgccaaa acacaacctg
     6121 gtcacgctca cctggggcaa tgtcagcgcc gttgatcgcg ggcgcggcgt cctggtgatc
     6181 aaaccttccg gcgtcgacta cagcatcatg accgctgacg atatggtcgt ggtcagcatc
     6241 gaaaccggtg aagtggttga aggtacgaaa aagccctcct ccgacacgcc aactcaccgg
     6301 ctgctctatc aggcattccc gtctattggc ggcattgtgc acacacactc gcgccacgcc
     6361 accatctggg cgcaggcggg tcagtcgatt ccagcaaccg gtaccaccca cgccgactat
     6421 ttctacggca ccattccctg cacccgcaaa atgaccgacg cagaaatcaa cggtgaatat
     6481 gagtgggaaa ccggtaacgt catcgtagaa accttcgaaa aacagggtat caatgcagcg
     6541 caaatgcccg gcgtgctggt ccattctcac ggcccgtttg catggggcaa aaatgccgaa
     6601 gatgcggtgc acaacgccat cgtgctggaa gaagtcgctt atatggggat tttctgccgc
     6661 cagcttgctc cgcagttacc ggatatgcag caaacgttgc tggataagca ctatctgcgt
     6721 aagcatggcg caaaagccta ttacggccag taatgactgt ataaaaccac agccagtcaa
     6781 ccaaaaccag gctatactca agcctggttt tttattggat tttcagcgtg gcgcaggcag
     6841 gttttatctt aacccgacac tggcgggaca ccccgcaagg aaccgaagtt tccttttggt
     6901 tagcgacgga caacgggccg ttgcaggtta cgcttgcgcc gcaagagtcc gtggcgttta
     6961 ttcccgccga tcaggttccc cgcgcccagc atattttgca gggtgagcaa ggctttcgcc
     7021 tgacgccgct ggcgttaaag gattttcatc gccagccggt gtatggcctt tactgtcgcg
     7081 cccatcgcca gttgatgaat tacgaaaagc gcctgcgtga aggtggcgtt accgtctacg
     7141 aggccgatgt gcgcccgcca gaacgctatc tgatggagcg gtttatcacc tcgccagtgt
     7201 gggtcgaggg tgatatgcgc aacggcgcta tcgttaatgc ccgtctgaaa ccgcatcccg
     7261 actatcgtcc gccgctcaag tgggtttcta ttgatattga aaccacccgc cacggtgagc
     7321 tgtactgcat cggcctggaa ggttgcgggc agcgtatcgt ttatatgctg gggccagaga
     7381 atggcgacgc ctccgcgctc gattttgaac tggaatacgt cgccagccgc ccgctgctac
     7441 tggaaaaact taacgcctgg tttgcgactc atgatcctga tgtgatcatc ggttggaacg
     7501 tggtgcagtt cgatctgcga atgctgcaaa aacatgccga gcgttaccgt attccgctgc
     7561 gtctggggcg tgataacagc gagctggagt ggcgcgagca cggctttaaa aacgacgtct
     7621 tttttgctca ggccaaaggg cgactgatta tcgacggtat cgaggcgctg aaatccgcgt
     7681 tctggaattt ctcttcattc tcgctggaaa ccgtcgctca ggagttatta ggcgaaggaa
     7741 aatctatcga taacccgtgg gatcgaatgg acgaaattga ccgccgtttc gccgaagata
     7801 aacctgcgct ggcaacttat aacctgaaag attgcgagct ggtgacgcag atcttccaca
     7861 aaactgaaat catgccgttt ttgctcgaac gggcgacggt gaacggcctg ccggtagatc
     7921 gacacggcgg ttcggtggca gcgtttggtc atctctattt tccccgtatg catcgcgctg
     7981 gttatgtcgc gcctaatctc ggcgaagtgc cgccgcacgc cagccctggc ggctacgtga
     8041 tggattcgcg acctgggctt tatgattcgg tgctggtgct ggactacaaa agcctgtacc
     8101 cgtcgatcat ccgcaccttt ctgattgatc ccgtcgggct ggtggaaggc atggcgcagc
     8161 ctgatccaga gcacagtacc gaaggttttc tcgatgcctg gttctcacgg gagaaacatt
     8221 gcctgccgga gattgtgact aacatctggc acgggcgcga tgaagccaaa cgccagggta
     8281 ataagccgct ttcgcaggcg ctgaagatca ttatgaatgc cttttatggt gtgctcggca
     8341 ccaccgcctg ccgtttcttc gatccacgcc tggcatcgtc gatcaccatg cgtggtcatc
     8401 agatcatgcg gcaaaccaaa acgttgattg aagcgcaggg ctatgatgtt atctacggcg
     8461 ataccgactc aacgtttgtc tggctgaaag gtgcacattc ggaagaagaa gcagcgaaaa
     8521 tcggtcgtgt actggtgcag cacgttaacg cctggtgggc ggaaacgctg caaaaacaac
     8581 ggctgaccag cgcactggaa ctggagtatg aaacccattt ctgccgtttt ctgatgccaa
     8641 ccattcgcgg ggccgatacc ggcagcaaaa agcgctacgc cggactgatt caggagggcg
     8701 acaaacagcg gatggtgttt aaagggctgg aaaccgtgcg tacggactgg acgccgctgg
     8761 cccagcagtt tcagcaggag ctttacctgc gcatcttctg caacgagcca tatcatgaat
     8821 acgtgcgcga aaccatcgac aagctgatgg cgggcgaact ggatgcgcgg ctggtttacc
     8881 gtaaacgcct tcgccgtccg ctgagcgaat atcagcgtaa tgttccgcct catgtacgcg
     8941 ccgctcgcct tgccgatgaa gaaaaccata agcgcggtcg ccccttgcaa tatcagaacc
     9001 gcggcaccat taagtacgta tggaccacca acggcccgga gccgctggac taccaacgtt
     9061 caccactgga ttacgaacac tatctgaccc gccagctaca acccgtggcg gagggaatac
     9121 tcccttttat tgaggataat tttgctacac ttatgaccgg gcaacttggg ctattttgag
     9181 caaaaaaaag agttcgccag ataccatttt gatgcgtgac gaatgctttg ccatccagta
     9241 ccatagcgcc ctttccattc ctggacctga ataacaccac tacctcataa gcacggtagc
     9301 gggtggttat tgcctgcaat taaagatata gagccgaaca catatgcctt ttacacttgg
     9361 tcaacgctgg atcagcgata cagaaagcga attgggactt ggaaccgttg tcgcggtgga
     9421 tgcgcgaact gtcactttac ttttcccatc tactggtgaa aaccgtctgt acgcacgcag
     9481 tgattccccc gtgacccgcg tgatgttcaa ccctggtgat accattacca gccatgacgg
     9541 ctggcagatg caagtcgaag aagtaaaaga agaaaatggc ttgctgacct atatcggtac
     9601 tcgccttgat actgaagagt ccggcgtagc cctgcgtgaa gttttccttg atagcaaact
     9661 ggtgttcagc aaaccgcagg accgtctgtt tgccgggcag attgaccgta tggaccgctt
     9721 tgcgctgcgt tatcgcgcgc gtaagtattc cagcgaacag ttccgtatgc cgtacagcgg
     9781 cctgcgcggt cagcgtacca gcctgatccc gcaccagctc aacatcgctc atgatgttgg
     9841 tcgccgccac gcgccgcgcg tcctgctggc tgacgaagtg ggtttaggga aaaccattga
     9901 agccgggatg atcctgcatc agcaactgct ctctggcgct gctgaacgtg tgctgattat
     9961 cgtcccggaa accttacagc atcagtggct ggtagaaatg ctgcgccgtt tcaacctgcg
    10021 ctttgcgcta tttgatgatg agcgttatgc cgaagctcag cacgatgctt acaacccgtt
    10081 cgacaccgaa cagctggtga tttgctcgct ggattttgcc cgtcgtagca aacagcgtct
    10141 ggaacatctc tgtgaagccg agtgggacct gctggtggtc gatgaagcgc atcacctggt
    10201 gtggagcgaa gatgcgccga gccgtgaata tcaggccatt gaacaactgg cagagcacgt
    10261 gcccggcgtt ctgctgctga ccgcaacccc ggaacaactg gggatggaaa gccacttcgc
    10321 ccgtctgcgt ctgctggacc cgaaccgttt ccacgatttt gcgcaattcg ttgaagagca
    10381 gaaaaattat cgtccggttg cggacgccgt tgccatgctg ctggctggta acaaactcag
    10441 caatgacgaa ctgaacatgc tcggtgagat gatcggcgag caggatatcg agccgctgtt
    10501 gcaagcagca aacagcgaca gcgaagatgc ccagagcgcc cgtcaggagc tggtttcgat
    10561 gctgatggat cgccacggca ccagccgcgt gctgttccgt aacacccgta acggtgtgaa
    10621 aggcttcccg aaacgcgagc tgcacaccat taagctgccg ctgccgacgc agtatcagac
    10681 ggctattaaa gtctccggca ttatgggcgc acgcaaaagt gcggaagatc gcgctcgcga
    10741 tatgctctac ccggagcgta tttatcagga atttgaaggt gataacgcca cctggtggaa
    10801 cttcgatcca cgcgttgagt ggctgatggg ctacctgacc agccatcgct ctcagaaagt
    10861 gctggtgatc tgcgccaaag ctgccactgc gctgcaactg gagcaggtac tgcgagaacg
    10921 tgaaggtatt cgcgccgcgg tattccacga aggtatgtcg attatcgaac gtgaccgcgc
    10981 tgccgcctgg tttgccgaag aagacaccgg cgcgcaggta ctgctgtgct cggaaatcgg
    11041 ttctgaagga cgtaacttcc agttcgccag ccacatggta atgtttgacc tgccattcaa
    11101 cccggatctg ctggagcagc gtattgggcg tctggatcgt atcggtcagg cgcacgatat
    11161 tcagatccat gtgccttatc tggaaaaaac tgctcagtcg gtgctggtgc gctggtatca
    11221 cgaaggtctg gatgcattcg agcacacctg cccgaccgga cgcactattt acgatagcgt
    11281 atacaacgat ctgattaact atctggcttc accggatcaa accgaaggct ttgacgatct
    11341 gatcaaaaac tgccgcgagc aacatgaagc gctgaaagca cagctggaac aaggtcgtga
    11401 ccgcctgctg gaaatccatt ccaacggtgg cgaaaaagcc caggcactgg cagaaagcat
    11461 tgaagagcag gatgacgata ccaacctgat cgccttcgcc atgaacctgt tcgatattat
    11521 cggtatcaat caggacgatc gcggcgacaa catgatcgtg ctgacgccgt ccgatcatat
    11581 gctggtgccg gacttccctg gcctgtcgga agatggcatc accatcacct tcgaccgtga
    11641 agtggcgctg gcgcgtgaag atgcacagtt tattacctgg gagcatccgc tgatccgcaa
    11701 cggtctggat ctgatccttt ctggcgatac cggtagcagc acgatttcac tgttgaaaaa
    11761 caaagcgttg ccggtaggta cgctgttggt ggaactgatt tatgtggttg aagcccaggc
    11821 tccgaagcag ttgcagctca accgcttcct gccaccgacg ccggtacgta tgctgctgga
    11881 taaaaacggc aacaacctgg cggcgcaggt agagtttgaa acctttaacc gccagcttaa
    11941 cgcggttaac cgtcacaccg gcagcaagct ggttaacgcg gtgcagcagg atgttcacgc
    12001 tatccttcag cagggggaag cgcagatcga gaaatctgcc cgtgcattga ttgatgcagc
    12061 gcgtaacgaa gccgacgaaa aactgtctgc cgagctgtct cgtctggaag cgctgcgtgc
    12121 agttaacccg aacattcgtg acgacgaact gaccgccatt gagagcaacc gtcagcaggt
    12181 aatggaaagc ctggatcagg caggctggcg tctggatgcc ctgcgtttga tcgttgtgac
    12241 gcatcagtaa cggagccgaa gatggggatg gaaaactaca atccgccgca ggaaccctgg
    12301 ctggttgtcc tgtatcagga tgaccatatt atggtggtca acaagccgag cggtttgttg
    12361 tctgtgccgg gtcgtctgga agagcacaaa gacagcgtga tgacgcgcat tcagcgtgat
    12421 tatccgcagg cagaatcggt gcatcgtctg gatatggcta ccagcggcgt gattgtagtg
    12481 gcgctgacca aagccgcgga gcgggagtta aaacgccagt tccgcgagcg cgagccgaaa
    12541 aaacagtatg tggcccgcgt ctgggggcat ccatcccctg cggaaggtct ggtggatctg
    12601 ccgctgattt gcgactggcc aaaccgcccg aaacagaaag tctgttacga aacaggtaaa
    12661 cctgcgcaga cggagtatga agtggtggaa tatgcggcgg ataacacggc aagagtggtg
    12721 ttaaaaccga ttaccgggcg ttcgcatcag ctgcgtgtgc atatgctggc gctgggtcat
    12781 ccgattctcg gcgatcgttt ttatgcatca ccagaagcga gagcaatggc accgcgtttg
    12841 ttgctacatg cagagatgct gacgattacc catccggcgt atggcaatag tatgacattt
    12901 aaagcaccag cggattttta atagtcacgc tggcgaagtt cgataggccg gagaaggcgt
    12961 tcacgccgca tccggcaatt ttgcgccgat tgcctgatgc gacgcgggcc gcgtcttatc
    13021 aggcctacac gttgaaactt atttaaaccc tttctgctgc tttatcagct cataggcctg
    13081 ctgaatttcc tgcgctttct gcttcgccat ctccatcatc tctggcggca aacctttcgc
    13141 caccaactta tcgggatggt gttcactcat cagcttacgg taggcacgtt tgatggtggt
    13201 cgcatcatcc gtcggcttca cgcccagcac attacaggca tcttccagcg tcgggccacg
    13261 ctgcgcttgc tgccagttac caccgccaga ttgctgctga taaccgccgc caaactgtgc
    13321 accgccctgc atcatgcgca aaaactggtc aaactgagcg cgggagatcc ctaattcttc
    13381 agcaatgaca tacagcaccg cccgttcatt cgggtgcagt gaaccatcag caaacgccgc
    13441 ctgaatctgg atctccagaa acatacgaat taagtcaaaa cgaccaaagc agacactgcg
    13501 aaactggcgc atcttttcgc gcagcgggta attgtctgat tttcccaccc ggaacgcatt
    13561 ttgcgccgca gtacgggaag cgccatgaag gttcattcgg tccatcaact ggctggcgat
    13621 atgaatatca gcttccgtga cgcgaccttt ggatttggtt aaatgcccca tcacttcaaa
    13681 agtggtggca aaaaacagcg cctgacgctc acgctggttg gcgaaccacg ccattttacg
    13741 gctacgggct ttatcaaaca tatggccaat taacaggccc aatactacgc cccaaaagcc
    13801 gccgcccatt attaaggcca cggtcacgcc aatgattttt ccccaatact gcatatattc
    13861 cccaaatcga cacacggata tcagggctat ctcccacaat ataaaggcgc ttttacccat
    13921 ttctggctgc ggtctattgt gagacatcgc ctataatttg cattatcata cctgtcattc
    13981 aatgccgtgc ctaacaccac agacgctatt cggacaggat taacactagc gtagagatga
    14041 cgagtacgtt agtctctgac cgtttgtcac gcgcaacgtt accgatgatg gaacaataaa
    14101 atcaacgtat gaaaaaacgt atccccactc tcctggccac catgattgcc accgcccttt
    14161 atagtcaaca gggactggca gccgacctcg cctcacagtg catgttgggc gtgccaagct
    14221 atgaccgtcc tctggtacag ggcgatacca atgacttacc cgtgactatc aatgctgacc
    14281 acgcgaaagg ggactacccg gatgacgccg tgtttactgg cagcgtggat atcatgcagg
    14341 gtaacagccg tctgcaggcc gacgaagtgc agctccatca aaaagaggca ccaggacaac
    14401 cggagccggt gcgtaccgtt gatgcgctcg gtaatgtcca ttacgacgat aaccaggtga
    14461 tcctcaaagg gccgaaaggc tgggcgaatc tgaacaccaa agataccaac gtctgggaag
    14521 gtgattacca gatggtaggt cgccagggtc gcggtaaagc ggacctgatg aaacaacgtg
    14581 gcgaaaaccg ctataccatt ctggataacg gtagctttac ctcctgtctg ccgggttctg
    14641 acacctggag cgtggtaggt agcgaaatta ttcatgaccg cgaagaacaa gttgcggaga
    14701 tctggaacgc ccgctttaag gtgggtccgg taccgatctt ttatagcccg tatttgcagt
    14761 tgccggtggg tgacaaacgt cgctctggtt tcttgatccc gaacgccaag tacaccacca
    14821 ccaactactt tgagttctac ctgccatatt actggaacat cgcgccaaat atggatgcca
    14881 ccatcacgcc gcattatatg catcgtcgtg gcaacatcat gtgggagaac gaattccgct
    14941 atctctccca ggcgggcgct ggcttgatgg aactggacta tctgccttca gataaagtct
    15001 ataaagatga acacccgaac gatgacagtt cacgtcgttg gttgttctac tggaaccact
    15061 ccggggtcat ggatcaggtg tggcgtttca acgtcgacta caccaaggtc agcgatccta
    15121 gctacttcaa tgacttcgat aacaaatacg gttccagtac cgacggctac gcaacgcaaa
    15181 aattcagcgt tggctatgcg gtgcaaaact tcaatgccac cgtttcaacc aagcagttcc
    15241 aggtcttcag cgagcagaac accagtagct actcggcaga gccgcagtta gacgttaact
    15301 actaccagaa tgatgttggt ccgtttgata cgcgtattta cggccaggca gtgcactttg
    15361 ttaacaccag agatgacatg ccagaggcga ctcgtgttca cctggaaccg accatcaatc
    15421 tgccgctctc taataactgg ggcagcatca atactgaagc gaagttgctg gcaacccatt
    15481 atcagcaaac caatcttgac tggtataact ccagaaacac gaccaggctg gcagagtccg
    15541 ctaaccgtgt aatgccgcag ttcaaggtag acggtagaat ggtcttcgag cgtgatatgg
    15601 aaatgctggc gccgggctat acccagacac tggaaccacg cgcgcagtat ctgtatgtgc
    15661 catatcgcga tcagagcaaa atctataact acgactcgtc gctgctgcaa tctgactact
    15721 ccggcctgtt ccgggaccgg acttacggcg gtcttgaccg tattgcctcc gctaaccagg
    15781 tgacgaccgg tgtcacatct cgcatatatg atgatgctgc cgttgaacgt tttaatattt
    15841 ccgttggtca aatctactat ttcacggagt ctcgcactgg cgatgacaac ataacatggg
    15901 agaatgacga caaaacgggc tcactggtat gggcaggcga tacttactgg cgcatctctg
    15961 atcgttgggg attgcgtggc gggattcagt acgatacacg tctggataac gtagcgacca
    16021 gtaactccag cattgaatac cgtcgggatg aagaccgtct ggtacagctg aattaccgtt
    16081 acgccagccc ggaatatatt caggcaacgc tgcctaagta ctattccact gctgagcaat
    16141 ataagaatgg tatttcgcag gtaggtgccg tcgccagctg gccaattgcc gatcgttggt
    16201 ccattgttgg ggcctactac tacgacacca atgcgaacaa gcaagccgac tctatgttag
    16261 gtgtgcaata cagctcctgc tgctatgcga ttcgcgtcgg ttacgagcgg aagctgaacg
    16321 gttgggataa cgataaacaa catgcggtat atgacaacgc aatcggcttt aacatcgaac
    16381 ttcgcggcct gagctccaac tacggtctgg gtacgcaaga gatgctgcgt tcgaacattc
    16441 tgccgtatca aaacactttg tgatttgttg atttaccacg taatccgcag tgcggttaat
    16501 tgaaatggaa aaagtatgaa gaactggaaa acgctgcttc tcggtatcgc catgatcgcg
    16561 aataccagtt tcgctgcccc ccaggtagtc gataaagtcg cagccgtcgt caataacggc
    16621 gtcgtgctgg aaagcgacgt tgatggatta atgcagtcgg taaaactgaa cgctgctcag
    16681 gcaaggcaac aacttcctga tgacgcgacg ctgcgccacc aaatcatgga gcgtttgatc
    16741 atggatcaaa tcatcctgca gatggggcag aaaatgggag tgaaaatctc cgatgagcag
    16801 ctggatcagg cgattgctaa catcgcgaaa cagaacaaca tgacgctgga tcagatgcgc
    16861 agccgtctgg cttacgatgg tctgaactac aacacctatc gtaaccagat ccgcaaagag
    16921 atgattatct ctgaagtgcg taacaacgag gtgcgtcgtc gcatcaccat cctgccgcag
    16981 gaagtcgaat ccctggcgca gcaagtgggt aaccaaaacg acgccagcac tgagctgaac
    17041 ctgagccaca tcctgatccc actgccggaa aacccgacct ctgatcaggt gaacgaagcg
    17101 gaaagccagg cgcgcgcgat tgtcgatcag gcgcgtaacg gcgctgattt cggtaagctg
    17161 gcgattgctc attctgccga ccagcaggcg ctgaacggcg gacagatggg ctggggccgt
    17221 attcaggagt tgccggggat cttcgcccag gcattaagca ccgcgaagaa aggcgacatt
    17281 gttggcccga ttcgttccgg cgttggcttc catattctga aagttaacga cctgcgcggc
    17341 gaaagcaaaa atatctcggt gaccgaagtt catgctcgcc atattctgct gaaaccgtcg
    17401 ccgatcatga ctgacgaaca ggcccgcgtg aaactggaac agattgctgc cgatatcaag
    17461 agtggtaaaa cgacttttgc tgccgcagcg aaagagttct ctcaggatcc aggctctgct
    17521 aaccagggcg gtgatctcgg ctgggctaca ccagatattt tcgatccggc cttccgtgac
    17581 gccctgaccc gcctgaacaa aggtcaaatg agtgcaccgg ttcactcttc attcggctgg
    17641 catttaatcg aactgctgga tacccgtaat gtcgataaaa ccgacgcggc acagaaagat
    17701 cgtgcatacc gcatgctgat gaaccgtaag ttctcggaag aagcagcaag ctggatgcag
    17761 gaacaacgtg ccagcgccta cgttaaaatc ctgagcaact aatggttaaa acccaacgtg
    17821 ttgtgatcac tcccggcgag cccgccggga ttggcccgga cttaattgtc cagcttgcac
    17881 agcgtgagtg gccggtcgaa caggttgttt gtgccgatgc cactctcctt accgaccggg
    17941 cagcgatgct cggtttgccg ctcaccctcc gcccttattc ccccaactcc cctgcacaac
    18001 cgcaaactgc gggcacatta acgctacttc ctgtcgcgct acgtgaatct gtcactgtgg
    18061 ggcagttagc agttgaaaat gggcactatg tggtggaaac gctggcgcga gcgtgtgatg
    18121 gctgtctgaa cggtgaattt gctgcgctga tcacaggccc cgtgcataaa ggcgtcatta
    18181 acgacgcagg cattccgttt accggtcata ccgagttttt cgaagagcgt tcgcaggcga
    18241 aaaaagtggt gatgatgctg gcgaccgaag aacttcgcgt ggcgctggca acgacgcatt
    18301 taccgctgcg cgatatcgca gatgctatca cccctgccct tttgcacgaa gtgattgcta
    18361 ttttgcatca cgatttgcgc accaaatttg gtattgccga accgcgcatt ctggtctgcg
    18421 ggctgaatcc gcacgcgggc gaaggcggtc atatgggtac ggaagagata gacaccatta
    18481 ttccggtact caacgagctg cgggaacagg ggatgaaact caacgggccg ctgcctgccg
    18541 ataccctgtt tcagccgaaa tatctcgata acgccgacgc cgtgctggcg atgtaccacg
    18601 atcagggtct tcccgtgcta aaataccagg gcttcgggcg cggtgtgaac attacgctgg
    18661 gcctgccctt tattcgcaca tcagtggacc acggcaccgc gcttgaactg gcgggacgtg
    18721 gtaaagccga tgtcggcagt tttattacgg cgcttaatct cgccatcaaa atgattgtta
    18781 acacccaatg aataatcgag tccaccaggg ccacttagcc cgtaaacgct tcgggcaaaa
    18841 ctttctcaac gatcagttcg tgatcgacag cattgtttct gccattaacc cgcagaaggg
    18901 ccaggcgatg gtcgaaatcg gccccggtct ggcggcattg accgaaccgg tcggcgaacg
    18961 tctggaccag ctgacggtca tcgaacttga ccgcgatctg gcggcacgtc tgcaaacgca
    19021 tccattctta ggcccgaaac tgacgattta tcagcaggat gcgatgacct ttaactttgg
    19081 cgaactggcc gcgaaaatgg gccagccgct gcgtgttttc ggcaacctgc cttataacat
    19141 ctccacgccg ttgatgttcc atctgtttag ctatactgat gccattgccg acatgcactt
    19201 tatgttgcaa aaagaagtgg tgaatcgtct ggttgcagga ccgaacagca aggcgtatgg
    19261 tcgattaagc gtcatggcgc aatactattg caacgtgatc ccggtgctgg aagtaccgcc
    19321 gtcagccttt acaccaccac ccaaagtgga ttccgccgtc gtgcgcctgg ttcctcatgc
    19381 aacgatgcct cacccggtta aagatgttcg cgtgttgagc cgcatcacca ctgaagcctt
    19441 taaccagcgt cgtaaaacca ttcgtaacag cctcggcaac ctgtttagcg tcgaggtgtt
    19501 aacgggaatg gggatcgacc cggcgatgcg agcggaaaat atctctgtcg cgcaatattg
    19561 ccagatggcg aactatctgg cggagaacgc gcctttgcag gagagttaac gatgatcaat
    19621 tcgccccgag tgtgtattca ggttcaaagc gtctacattg aggcccaatc ttcacctgat
    19681 aatgaacgtt acgtttttgc ttataccgta accatacgca atctggggcg agcgccagtg
    19741 cagttgttgg ggcgttactg gctgatcacc aatggcaatg gccgtgaaac cgaagtccag
    19801 ggcgaaggag tggttggcgt ccagccactt atcgcgcctg gcgaagagta ccagtacacc
    19861 agcggtgcaa tcattgaaac cccgctgggc accatgcagg gtcactacga aatgatcgat
    19921 gaaaatggcg tccctttcag catcgacatt cccgtattcc gactcgccgt tcccacactc
    19981 attcattaaa agaatatggc gacatacctt attggcgacg ttcatggttg ttacgatgaa
    20041 ctgatcgcat tgctgcataa agtagaattt acccctggga aagataccct ctggctgacg
    20101 ggcgatctgg tcgcgcgcgg cccgggttcg ctggatgttc tgcgctatgt gaaatcctta
    20161 ggcgacagcg tacgtctggt gctgggtaat cacgatctgc atctgctggc ggtatttgcc
    20221 gggatcagcc gcaataaacc gaaagatcgc ctgacaccgc tgctggaagc gccggatgcc
    20281 gacgagctgc ttaactggct gcgtcgccag cctctgctgc aaatcgacga agagaaaaag
    20341 ctggtgatgg cccacgccgg gatcacgcca cagtgggatc tgcagaccgc caaagagtgc
    20401 gcgcgcgatg tagaagcggt gctgtcgagt gactcctatc ccttctttct tgatgccatg
    20461 tacggcgata tgccaaataa ctggtcaccg gaattgcggg ggctgggaag actgcggttt
    20521 atcaccaacg cctttacccg tatgcgcttt tgctttccaa acggtcaact ggatatgtac
    20581 agcaaagaat cgccggaaga agcccctgcc ccactgaaac cgtggtttgc gattcctggc
    20641 cccgtcgctg aagagtacaa catcgccttt ggtcactggg catcgctgga aggcaaaggt
    20701 acgccggaag gtatttacgc gctggatacc ggctgctgct ggggtggtac attaacctgc
    20761 ctgcgctggg aagataaaca gtattttgtc cagccgtcga accggcataa agatatgggt
    20821 gagggagagg cggtagcgtc ttaaatcccg tcgagcataa aatctaatgc tcctttaatc
    20881 aacgttcggt gcgcgctggc atcgcaaaat ttcgttccca gcgcgttaac tggaacagtt
    20941 gcagtctcat gcgtcatgag cacatattct ttgccgtcta ccagtaaaag gattgggtta
    21001 agccgttctg gtgggcgaat acggctaaat cgttcaatag gtgttaatgg aataacgata
    21061 cggcgattaa tctcaccaat aatgtcgctg gtaacatcaa taacaaaggg aaacgcggcg
    21121 ttcctgccgc gactgcggta taccgtaaat tgcatcgcta gaagctccgg tactcatcag
    21181 agaaacatcc ggtttcatca gccaattgat ttaacgcagc gatagcttct gcgttctctt
    21241 cacgccaacg tgttgctgca tgttttttaa gttcagcatt gagcgcaacc gtaagggtgg
    21301 cgctcatatt taagcctgca tcacgagcgc gattgactaa ctcacggtcg acggtgacgg
    21361 tcacactttg tgtggtacgt tttgcagtca tgatctcgtg ctcttgtaca atttatacgt
    21421 ctagtataca cggatatctg cacgtatcag tgatgccgga attctaatat acaaaattac
    21481 cgccgctcca gaatctcaaa gcaatagcta tgagagttct gcgcatcggc gtcgtggaat
    21541 tcactgaata ccgattccca gtcatccggc tcgtaatccg ggaaatgggt gtcgccttcc
    21601 acttccgcgt cgatatgcgt cagatacagt ttttgcgctt tcggcaggaa ctgctcataa
    21661 acgcgaccgc cgccaatcac catgatttct ggtacgtcac cacacgccgc aatggcttca
    21721 tccaccgact tcacccacgt tacgcgatcg tccgtacccg gttgactgct gaggataata
    21781 tttttgcgtc ctggcaacgg acgaccgatg gattcccagg tatggcggcc cataatcacg
    21841 ggtttattta aagtgttgcg tttaaaccag gcgagatcgg caggcaggtt ccacggcatg
    21901 gcgttttcca tgccgataac gcgatctacc gctaacgccg caatcagact gatcattgag
    21961 atttcccgat aaaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    22021 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctctccctgg
    22081 actcgccgca ttacaatgaa acaaaaacaa actgttagct gtaaagcgtg atttacgtca
    22141 ctctttatta ggatgagggc ttcgtttccg gttcatccgc catattgcca gtatgtttcc
    22201 cttcttcggt tccctgccag ccatgtcgtt gaattaatga cagatgttca cggtcttcgg
    22261 taatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcacgg gctttggtgt
    22321 cgttctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    22381 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagtgccaga cgcccggttt
    22441 tcagcgcacc ttcgaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    22501 tgtagtggtc gacatcgcgg gcgcgggcaa taatctgcaa atgtgggaaa tgttctttca
    22561 ccatctctgt cagttgcagg ttggtttgcg gatcgtcgat ggcgttaatc agcacttccg
    22621 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcacca taaaacactt
    22681 tcataccgaa tttacgcagc gtttcgatat ggtccgggcc gtgatcgagc accaccattt
    22741 tcaccccgct ggagagcagt aaacgtccgg ttatctgccc aaaacgcccg aatccggcaa
    22801 tgattacccg cggctgttct tcgtcgatct catcggcttc acgcgcctct tcagtagaag
    22861 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgccgccatc gacagtgcca
    22921 ccgccagagt cagcgatttc gcccactctg gctccagcac attcgccatc tgcgccgcac
    22981 caaatactac aaaggcaaac tcactgccct gtcctaacag caccgcaaac cagcgacgct
    23041 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    23101 ggaagccgag cagcaaaatg acaatgcgca atggtttttc aagcagcgtg ccaaagtcta
    23161 tcgacatgcc aacgccgatg aaaaacagcc ccagcagcaa acctttaaat ggttcgatat
    23221 cgctttccag cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    23281 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acgaggaata
    23341 acgccacggc actaaacact tcccgcaagc cagagcgggc aacaaaacgc agtgccggac
    23401 gcgtgacata gcgccccagc aataccacca gcaccagcgc gcccgccact tttaacgccg
    23461 agagaacaaa tgcgcccatc gtcgtcgagg cactgctcgc tgccagtagc gggatcatcg
    23521 ccaccagtgg gatcgccgcg atatcctgga acagcagcac cgcaaaggca ctgcggccca
    23581 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gtagaggaga
    23641 gcgccagcgt catgccgatc aattccgcga cctgccagcg cagcccaagt aacatgcaga
    23701 acagccccag caggccgccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    23761 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    23821 ccacgccaat ctctgcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    23881 acgggccaat aatgcagccg gcgatcaggt agccaagaac cgatcccaga ccgagacgta
    23941 ccgcaatggg aacaatcagc gccgccgaac cgagataaat cagcgcctga atcagcgtat
    24001 ggctatccat gatgtgcctc ctgccattcc agcagacgtt gcttatagtg ccgcgcctgc
    24061 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    24121 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    24181 tgcgcaccaa tctcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    24241 ttgccgtgca gcgccgtgcc gccgtgaccg taagcccatc cgtgcgagaa aactttatcg
    24301 atccaaagtt tgaggagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    24361 agatcggcac gagacagcgc ctcctgttcg gcggcaatgt cgatattgaa gtcaggatag
    24421 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    24481 ttattcgcat gggaatgatg cggatatgga tgcgcataaa ttataagaat cataggtagc
    24541 ccgcgtgatt acgcctttgt tttcccacaa agtgtagtca tatattcacg cagcttatag
    24601 tcaataatat tgattcagaa aagagagaaa tctgaacaaa ttaccccatt ctctgcccgc
    24661 ggatcgtcat attgctggtc tgcgccagtg acatccctcg agtctccggg gcaaacgcta
    24721 cggaaatcaa caagccaaac agcgagatac ccgcccccat tagcatcgtg ttactgatac
    24781 cgtaattatt gataaagatc ggcagtgccc aggtcgatac aatagtgcca atacggctta
    24841 aggacataat cacgcccacg gcagaggcgc ggatatccgt cgggaagagt tcattaggat
    24901 agagccactg caaattaccc ggcccaccag agaaaaaggc atacaccgca aaggccatca
    24961 ctaccagcca gatccccata tccgggatca gccccaaaac cgccagcgcc agcgtcatca
    25021 tggcaaagct gccaatcaac aatggacgcc gtccggcagt gtttagccac agcatcggcg
    25081 gaatacagcc gagcataaag aacagactaa tcaccacgtt ccccagcgcc gcgtttttgc
    25141 caacccccaa tcctaacaaa ccaacgattt gcgggccaaa ggtgtaaatg gcgaacattg
    25201 ggatcacctg gcaggtccag atggcggcaa caaacagaac aaaaggaaaa tggcggcgat
    25261 taaacagatc gcgaaaacgg gtttgctgcg gctgctcttc ctcgaaagcc accggttcgc
    25321 caaacagttt tatcatcatc tcttcgcact cttttactcg ccctttgcgt aacaaccagc
    25381 ggggagattc aggcagttcg aatcgaccaa tcaaaatcaa taaacaggga atcgctgcgc
    25441 tacccagcat ccagcgccag ccgccttcca catcataaag ccaatagccg accaaatcgg
    25501 cgcaggtcgc gccgacatac cacatcgcgg cgataaagct gatggaaaac gcccgctgac
    25561 gggtactgga gaactcggtg atcattgagg tggcgatggg ataatccgca ccgataacaa
    25621 tgccgataag tacccgcatc accaacagtt cgacggggga tgagacaaac atcgtcgcca
    25681 ccgatatcac gccgatggcg atgatatcaa tgaggaacat tttgcgccgt ccgactttat
    25741 cggagatata gccaaacagc gatgtgccaa cgaacagccc ggcgagcgtt cccgcgccca
    25801 gcaagccaat ccagtcagcg tccagtttca gcgccggagt cagttgctcc agcgccacgc
    25861 caatcattac cagtacataa ccatccagaa acggaccacc gcttccccac agcaaaatgc
    25921 ggcggtgaat agaggagaat ttaagatcat caaagtttct ggacggttgc atgggttatt
    25981 cctgtttttt tgcaatgaga gtcagtacgg cattacgtat caaaccaaca tcagccgtaa
    26041 cggaactcca caccaaaggt gccgcgcggg tattcccact gttccagcgc cgaccccagc
    26101 cccagaatgc gacaggtgcc gcactccaga catccggcgt aatcgaagcg cacactgccg
    26161 tcatcctgct tcttgtacag acctgcgggg cacgctttca ccagcagctc cagggcctgt
    26221 ttatcagcat cggccttcac aacgatgtgc ggatgctctt catcgacatt gaatttattg
    26281 acgcccagtt tgacgtccac attgacggga gaagtcataa aacggtcact cctttcatgc
    26341 catccttgat cagattgatg aagcccactt tcttgccgtg gcggaggatt ttcttgcgca
    26401 tcagttccgg cgcactgccg tcaatggtga acaggtcacg cgccacgccc accgccagtt
    26461 ccgggtagcc gctaaacatg cgtgggttat caaggaacgc cggtagtttc tggtacatgc
    26521 gcatatcgcg cagcgggcca ctctcaagat gctgacgata ttccgcgagt ttttgcttac
    26581 tgaaatcgtc gcttttcatc gctgaaagca cagtttttgc tgctgcttcc ccggcggcaa
    26641 tcgccagatc cataccgcga atggtaaaac cgaggttcat acacattccg gcagcatcac
    26701 cggcaatcaa tacgccgtca ccaaccaact ccggcagcat gttgatacct gcttccggca
    26761 ctacgtgagc ggaatattcc accagcttgc cgcccgcgat cagcggtgca acggccggat
    26821 gttgtttgaa atcttccagc atttgcggca ccgatttttt tgcgtcatgc agatgatgca
    26881 aaccacaaac cagccccagc gacagggtat tttcattggt ataaaggaag ccaccgccca
    26941 tcaggccatc ggtgggtgat cccgcaaaca ggcaagccgc gccctgatta ccctgcaact
    27001 gaaaacggtc ttcgataacc gacttcggta actcgatcag ttcctttacg ccaaccgcca
    27061 catccgtcgg tttaacgcgt tttgccatcc ccagcttttc ggcaaggatg gaattcaccc
    27121 catcagcaag gatcaccgtt ttcgcttcaa tcacatcgcc atcggcttct acaccgacga
    27181 ctttgccatc gcgctgtacg aggttatcga cgcggatccc ggtaattaac tgcgcgcccg
    27241 cttcttcagc ctgctccatc agccaggcat caaatttact gcgcaaaacg gagtaagaac
    27301 gctgggatgg cgaggtttcg tcgccattgc agtagtccat agtcatcgct gacttttccg
    27361 tcataaacgc gagtttttca tgggtgatca ggcgttctac gggggcggag tcagcgaaac
    27421 caggaataat gtgttccaga ctgtgggcat agagacgccc gccggtgacg ttcttggcac
    27481 ctgcggaatt gcctcgctcg ataactaaca cttgcgcacc ttcgcgggcg agcactagtg
    27541 cggcaaccga accggcaagc cctgcaccga cgatgatggc gtcaaagata tcttcggaca
    27601 taagagctcc ctgtcagcgg cagggccaaa atgcagccct gccagagtgg atcaacgcgc
    27661 taaagctgcg gtcagtgccg gaaggatctt cacggcgtcg ccaacaatgc cgtaatccgc
    27721 gtactggaag atcggcgcat ttttatcttt gttgatggcg aaaatggttt gcgacgcatt
    27781 agcgccaacc atgtgctgga tctgcccgga gatccccacc gccaggtaca gttcaggttt
    27841 cagcatcagg ttggagatac cgacatagcg ttcgtgctcc atccattttt cgttttccgc
    27901 caccggacga gaacaggcca actccgcacc tatcgccttg caaagctgtt ctgccagcgc
    27961 aatgttctct ttgctgccaa taccgcgacc aacgctgacc accaggcggg ctttgtcgag
    28021 atcgacgctg ttgctctgac gcgcctgggt tgccgtgcgg gtaatcgcca cagccggagc
    28081 ctgccactcc acggtgtgcg tttcgccagt gcgtgacgcg tctggctgag ccacatcgaa
    28141 cgtgccgctg ctgatggtca gaaccgcata cggcgtggca atgcgttctt cgccaatcgc
    28201 cagaccgccg taaaccatgt gtttcactgt cgctttaccg tcctgcacgc taacagcgct
    28261 ggcatcgtta gacaccgccg cgttaaggcg gtaacccagt ttcgccgcca gtaatttgcc
    28321 gcgacgggtg tttggcaaca gcaccagacc gtctgcgccg tgctggcgaa tagtgtcagc
    28381 catgacaccg gcgtaatctt cgatcatccg ctcgtccggt ttgccgctta atttccagac
    28441 atgattagcg ccgagctgga ttgcctgtgc gccgtcggca tcattgagga cgaacgtgtt
    28501 gatttgatta gctaaagcct gcgcaccgtt catcagttcc ggcagacgag aaggggtatc
    28561 gctgaatacc cagacttgag aaaacgtgtt catagcatcc cctgtaatta aatgactttg
    28621 cgaagatttt cagcaaatgc ggcgatctgt tcttcgccgt cgccttcaat cacgatgcgc
    28681 tgacgctcgc gctgtttcgg cgcggcaacc tgttgttctg accaggctgc ctctgcgtta
    28741 aaaccgatat ccgccgccga ccatacctgg actggctttt tcgccgcgcc gagaatggct
    28801 ttcatcgaag gaatttgtgg ggagttgata tcagtggaaa cagcaacaac cgcaggcagc
    28861 ggaatgctta aggtttctgt ttcatcttcc agttcgcgct caacggtgag ggtatctgca
    28921 gtcagggaga taattttgct gacgccgtta actgccggaa tattaaggat ttcgcctacc
    28981 agcaaaccaa cctgctgggc ataaaggtcg gaagaaccat cgccacagag gatcaaatca
    29041 aagcctgctt tctgagcggc tgcagccagt acgctcgccg tttgttgcgg cagtgcctgc
    29101 tcgaactggt catcaatcac cacaatcagt tcatccggac cgcgcgatag cacatcttta
    29161 cgccctttgg cgttggtcag ggctttaccg cccacactta aggccgtcac ctgcgcctct
    29221 gctgcctgtt gctttagctg gcaagccgct tcaatagcgt tgagatcgta ttggcttatt
    29281 ttggcatcgg ctttgctgaa gtctaacgaa ccatcagcat tattgaccgc aatatcctgt
    29341 tcatcaggca cgcacttata gcaagtaata atcttcattg catctccaga aatcatgaag
    29401 gaaacgtcgt tcatatcggt ggtgttaatt acagatatca cgccctttaa tttggaaata
    29461 aaactttaat caccaatatt gaaaatgtca cacgcagata aatccaactt tcaatattgt
    29521 taagctcctc accaatattg aaaacat
//
LOCUS       DAZFFE010000051        29495 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFFE010000051 DAZFFE010000000
VERSION     DAZFFE010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.051"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..515)
                     /gene="rfbC"
                     /locus_tag="ACHJ69_002477"
     CDS             complement(<1..515)
                     /gene="rfbC"
                     /locus_tag="ACHJ69_002477"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFO5015440.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQRQFEEIVGRKIS
                     FVQDNHSKSKKGVLRGLHYQLPPFAQGKLVRCINGEVYDVAVDLRPESINYGKWVGII
                     LSAENKKQLWIPEGFAHGFLVISEFAEFAYKTTNYYSKESERGIRWNDPTINIQWPKM
                     GQYLLSPKDALA"
     gene            complement(520..1398)
                     /gene="rfbA"
                     /locus_tag="ACHJ69_002478"
     CDS             complement(520..1398)
                     /gene="rfbA"
                     /locus_tag="ACHJ69_002478"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO5015441.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGAHQSLIEASNFIATIEERQGLKVSCPEEIAFRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGD"
     gene            complement(1456..2355)
                     /gene="rfbD"
                     /locus_tag="ACHJ69_002479"
     CDS             complement(1456..2355)
                     /gene="rfbD"
                     /locus_tag="ACHJ69_002479"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFO5015442.1"
                     /translation="MNILLFGKTGQVGWELQRSLAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHF
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRMALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLKAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(2355..3440)
                     /gene="rfbB"
                     /locus_tag="ACHJ69_002480"
     CDS             complement(2355..3440)
                     /gene="rfbB"
                     /locus_tag="ACHJ69_002480"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO5015443.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNKEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNENKNIDVVLTICDL
                     LDEIVPKEQSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRK"
     gene            complement(3812..4705)
                     /gene="galF"
                     /locus_tag="ACHJ69_002481"
     CDS             complement(3812..4705)
                     /gene="galF"
                     /locus_tag="ACHJ69_002481"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO5015444.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKISRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(4947..5942)
                     /locus_tag="ACHJ69_002482"
     CDS             complement(4947..5942)
                     /locus_tag="ACHJ69_002482"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFO5015445.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            complement(6100..7494)
                     /gene="wcaM"
                     /locus_tag="ACHJ69_002483"
     CDS             complement(6100..7494)
                     /gene="wcaM"
                     /locus_tag="ACHJ69_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO5015446.1"
                     /translation="MPFKKLSRRTFLAASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPAGKTLHILGSLRGNGRGRFILQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     NHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVISNIK
                     ARNITPDFSKKAGIDNAAVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(7505..8725)
                     /gene="wcaL"
                     /locus_tag="ACHJ69_002484"
     CDS             complement(7505..8725)
                     /gene="wcaL"
                     /locus_tag="ACHJ69_002484"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO5015447.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLHHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(8722..10002)
                     /gene="wcaK"
                     /locus_tag="ACHJ69_002485"
     CDS             complement(8722..10002)
                     /gene="wcaK"
                     /locus_tag="ACHJ69_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO5015448.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACKLTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(10278..11756)
                     /gene="wzxC"
                     /locus_tag="ACHJ69_002486"
     CDS             complement(10278..11756)
                     /gene="wzxC"
                     /locus_tag="ACHJ69_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO5015449.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVVVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSSALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRVG"
     gene            complement(11758..13152)
                     /gene="wcaJ"
                     /locus_tag="ACHJ69_002487"
     CDS             complement(11758..13152)
                     /gene="wcaJ"
                     /locus_tag="ACHJ69_002487"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO5015450.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(13207..14577)
                     /gene="cpsG"
                     /locus_tag="ACHJ69_002488"
                     /gene_synonym="manB"
     CDS             complement(13207..14577)
                     /gene="cpsG"
                     /locus_tag="ACHJ69_002488"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO5015451.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEGNDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMSAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(14770..16206)
                     /gene="cpsB"
                     /locus_tag="ACHJ69_002489"
     CDS             complement(14770..16206)
                     /gene="cpsB"
                     /locus_tag="ACHJ69_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO5015452.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(16209..17432)
                     /gene="wcaI"
                     /locus_tag="ACHJ69_002490"
     CDS             complement(16209..17432)
                     /gene="wcaI"
                     /locus_tag="ACHJ69_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO5015453.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQAFLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(17429..17908)
                     /gene="gmm"
                     /locus_tag="ACHJ69_002491"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(17429..17908)
                     /gene="gmm"
                     /locus_tag="ACHJ69_002491"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO5015454.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTSDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(17911..18876)
                     /gene="fcl"
                     /locus_tag="ACHJ69_002492"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(17911..18876)
                     /gene="fcl"
                     /locus_tag="ACHJ69_002492"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO5015455.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIQAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMVESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWQENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(18879..20000)
                     /gene="gmd"
                     /locus_tag="ACHJ69_002493"
     CDS             complement(18879..20000)
                     /gene="gmd"
                     /locus_tag="ACHJ69_002493"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO5015456.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(20027..20575)
                     /gene="wcaF"
                     /locus_tag="ACHJ69_002494"
     CDS             complement(20027..20575)
                     /gene="wcaF"
                     /locus_tag="ACHJ69_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO5015457.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGANSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVMIGNGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(20591..21337)
                     /gene="wcaE"
                     /locus_tag="ACHJ69_002495"
     CDS             complement(20591..21337)
                     /gene="wcaE"
                     /locus_tag="ACHJ69_002495"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO5015458.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRKLKVQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYRAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            complement(21348..22565)
                     /gene="wcaD"
                     /locus_tag="ACHJ69_002496"
     CDS             complement(21348..22565)
                     /gene="wcaD"
                     /locus_tag="ACHJ69_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO5015459.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVVLAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNEEAIKTKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(22540..23757)
                     /gene="wcaC"
                     /locus_tag="ACHJ69_002497"
     CDS             complement(22540..23757)
                     /gene="wcaC"
                     /locus_tag="ACHJ69_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO5015460.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSDQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVCETQGKSKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(23754..24226)
                     /gene="wcaB"
                     /locus_tag="ACHJ69_002498"
                     /pseudo
     CDS             complement(23754..24226)
                     /gene="wcaB"
                     /locus_tag="ACHJ69_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
     gene            complement(24229..25068)
                     /gene="wcaA"
                     /locus_tag="ACHJ69_002499"
     CDS             complement(24229..25068)
                     /gene="wcaA"
                     /locus_tag="ACHJ69_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO5015461.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYNNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWIPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(25161..27323)
                     /gene="wzc"
                     /locus_tag="ACHJ69_002500"
     CDS             complement(25161..27323)
                     /gene="wzc"
                     /locus_tag="ACHJ69_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO5015462.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTIIEARWWVIGITAVFALC
                     SVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADRVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSVAFLAQQL
                     PEVRNRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVKWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(27326..27769)
                     /gene="wzb"
                     /locus_tag="ACHJ69_002501"
     CDS             complement(27326..27769)
                     /gene="wzb"
                     /locus_tag="ACHJ69_002501"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO5015463.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFTAVYTLLERSARQWAQALNAEQV"
     gene            complement(27775..28914)
                     /gene="wza"
                     /locus_tag="ACHJ69_002502"
     CDS             complement(27775..28914)
                     /gene="wza"
                     /locus_tag="ACHJ69_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO5015464.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGESGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            29223..29372
                     /locus_tag="ACHJ69_002503"
     CDS             29223..29372
                     /locus_tag="ACHJ69_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015465.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
BASE COUNT         7122 a         7853 c         7043 g         7477 t
ORIGIN      
        1 gctaacgcat ctttcggaga aagtaaatat tgtcccattt ttggccattg gatatttatc
       61 gttggatcat tccaacgtat gcctctttca ctttcttttg aataataatt tgtggttttg
      121 tacgcaaatt cagcaaattc gcttataact aaaaaaccat gtgcaaaacc ttctggtatc
      181 catagctgct tcttattttc agcagaaaga atgatgccta cccatttacc ataattaatt
      241 gattcaggtc gtagatcaac tgcaacatca tatacttcac cattgataca tctaactaat
      301 ttaccttgtg caaatggagg caactgataa tgaaggccac gtaaaacacc tttttttgac
      361 tttgaatgat tatcctgaac aaacgaaatt ttgcgtccaa caatttcttc aaattgtcgt
      421 tgattaaagc tttccataaa aaaaccacgt tcatccccaa acacttttgg ttcaaaaatc
      481 agtacatctg gaatttctgt tttaattacg ttcattttat taatcacctt taatcatttt
      541 gagaagatac tgaccataag catttttctt cagcggttcg gctaacactt tcacctgctc
      601 agcatcaata aaccctttcc ggaaagcaat ttcttccggg caggaaactt tcaagccttg
      661 acgctcttca attgttgcaa tgaagttgct tgcctcaatc aggctttgat gtgcccccgt
      721 gtccagccat gcataaccac ggcccatcat ggcaacagat aaacgcccct gttccatata
      781 gatacggtta atatcggtaa tttccagttc accacgagca gaaggcttaa gatttttcgc
      841 catttcgaca acgtcgttat catagaaata aagcccggtt accgcataat tactttttgg
      901 ttgtaacggt ttttcttcca ggcttattgc cgtaccgttt ttatcaaact caacgacacc
      961 gtagcgttca ggatcattaa cgtgataggc aaatactgtt gcaccacttt ctttgttaac
     1021 agcgacatcc attaacttcg gcagatcatg accgtaaaag atattatcac cgagaaccag
     1081 agcacaatca tcaccaccga taaactcttc accgataata aacgcctgcg caagcccatc
     1141 tggagtcggt tgcactttgt actgaagatt tagcccccac tggctaccgt cacccagcag
     1201 ttgttgaaaa cgaggagtat cctgtggcgt actaataatc agaatatcgc gaatacccgc
     1261 caacatcagt gtagagagcg ggtaatagat catcggctta tcataaatag gtaatagctg
     1321 tttactgaca gccatagtca caggataaag acgagtacca gaaccacccg ctaaaataat
     1381 acctttacgc gttttcattt catcattcct tttaattcat cttgctccac catcacgaac
     1441 aagatgcaaa aactattaaa ttgctgtagt cgtaaataat tcgttgagca ttcgtttcac
     1501 gccaacctgc cagtcaggca agacaagcgc aaaattctgc tgaaattttt ctgtattaag
     1561 gcgagagtta tgtggacgac gagctggtgt aggataggct gttgttggta ctgccttgag
     1621 cttgttgagt gcaaggggaa tacctgcttt tcgtgcctct tcaaaaacca gcgcagcata
     1681 atcgtgccag gttgtggtac cactggctac cagatggtac aagcctgcga cttctggttt
     1741 attcagtgcc atacgaattg catgtgccgt acaatcagcc agcagttcag cacctgttgg
     1801 tgcaccaaac tgatcgttaa tgacagctaa ttcttcgcgc tcttttgcca gacgtaacat
     1861 tgtcttggca aaattgtttc ctttacctgc atatacccag ctggtacgaa aaataaggtg
     1921 cttcgcacaa tgctcttgta atgctttttc tccagctaac ttggtttcac cgtaaacatt
     1981 tagtggtgcg gttgcatcag tctccagcca tggagtgtcg ccatttccag ggaatacgta
     2041 gtcagttgag aaatgaatta cccaggcccc aacttcatta gccgcttttg caattgattc
     2101 aacgctggtc gcattgagta attgtgcaaa ttcgggttct gactcagctt tatctactgc
     2161 ggtatgagcc gcagcattaa caataacatc tgggcgaatt tttttgacgg tttcagccac
     2221 accttctggg ttgctgaaat cgccacaata atcagtggag tgaacatcaa gagcaatcaa
     2281 gttacccaga ggagcaagag aacgctgtag ttcccaacct acctgccctg ttttgccgaa
     2341 aaggaggata ttcattactt gcggccctca tagttctgtt caatccacga ttgataggca
     2401 ccacttttca cattatcaac ccattttgta ttggacaggt accattccac tgtcttccga
     2461 atcccgctct caaacgtttc ctgcggtttc cagcccaatt cgcggctaat cttctcagca
     2521 tcaatcgcat aacggcgatc gtgtcccgga cgatcggcaa cataagtaat ttgctcacga
     2581 taagattgct ctttcggtac aatctcatcc aacaaatcac aaatagtcag cacaacatcg
     2641 atgtttttat tttcgttgtg tccgccaatg ttataagttt cacccgcttt accttcggtt
     2701 acgacggtat ataacgcacg cgcatgatct tcaacataca gccaatcgcg aatttgatcc
     2761 cctttgccat aaataggtaa tgccttacct tccagagcat taagaattac tagtggaatc
     2821 aatttttccg gaaagtgata aggaccatag ttattcgaac agttagtcac aatggtcggt
     2881 aaaccatagg tacgtttcca cgcgcgaact aaatgatcgc tggatgcttt tgatgcggaa
     2941 taaggactgc ttggcgcgta agctgtcgtc tcagtaaata agggtaattc ttctttatta
     3001 tttacttcgt caggatgagg cagatcgcca tagacttcgt cagtagaaat atgatgaaaa
     3061 cggaagctat ttttcttgtc gctatcaaga gcagaccagt aattgcgagc ggcttccaaa
     3121 aggacataag taccaacaat attggtttca ataaatgccg caggacctgt aattgaacgg
     3181 tcaacatggc tttcagcagc caggtgcatc actgcatccg gctgatgctg agcaaaaatc
     3241 cgcgccattg cagcagcatc gcaaatatcc gcatgttcaa aaacatagcg ttcagaatca
     3301 gaaacatcag caagtgattc caggtttccg gcgtacgtta atttatcgac attaacaaca
     3361 ctatcctgcg tattatttat aatgtgacga actacagcag aaccaataaa tcctgcgcca
     3421 ccagtaacaa gtatcttcac ttttttattc catattactt cagagcatgc tgtgaaatta
     3481 gcggctctta gtttgattaa tagaggtatt aatgcacgct accgcccctg gctttacagc
     3541 taccagagca ctgcatgcat gcctacgatg tgacgagcgt tacccactcg cgcttaactc
     3601 gaaaaattca aacgctaatt gtcttaccaa tccgctctgg gaacaaggaa aatcctggaa
     3661 aactttgact aaaatcctat tgctaactcg ttgttattct gattgtttat ataaaacaac
     3721 ggcaggaata ttcgcaacaa attactttcg ccacgaatct tcaccgccgt tataattttc
     3781 ttatcaaccg ttacatccgg tcagattttc attattcgct taacagcttc tcaatccctt
     3841 tacggaactt cgccccttct ttcaggttgc gtagtccata cttcacaaac gcctgcatat
     3901 aacccatttt tttaccgcag tcgtagctgt cacctgtcat cagcattgca tcaacagatt
     3961 gttttttcgc cagttcggca atagcatcag tcagctgaat acgtccccat gcaccaggct
     4021 gagtacgttc aagttccggc caaatatcgg cagaaagcac atagcgacca acggccatga
     4081 tgtctgagtc cagcgtctgc ggctgatctg gcttttcgat gaattcaaca atgcggctga
     4141 ttttaccttc acgatccagc ggctctttgg tctgaataac ggagtattca gagaggtcac
     4201 ctggcatacg ttttgccagc acctggctac ggcccgtttc attgaagcgc gcaatcatgg
     4261 cagcaaggtt gtagcgcagc gggtcggcgc tggcgtcgtc gatcactacg tctggcagta
     4321 ccacgacaaa tgggttgtca ccaatggcag gtcgcgcaca caaaatggag tggcctaaac
     4381 ctaaaggttc gccctgacgc acgttcataa tagtcacgcc cggcggacag atagattgta
     4441 cttccgccaa cagttgacgc ttcacacgtt gttcaagaag agattctaat tcataagagg
     4501 tgtcgaagtg gttttcgacc gcgttcttgg acgcatgagt taccaggagg atttctttga
     4561 tccctgcagc cacaatctcg tcaacaatgt actgaatcat tggcttgtcg acgatcggta
     4621 gcatctcttt gggaatcgcc ttagtggcag gcaacatatg catcccaaga cctgctaccg
     4681 gtataactgc ttttaaattc gtcattcttt cctacctcta agtgactgat agtgcgtaaa
     4741 ttattgccat aggttagcca aacgttatgg ctatatacca aagcttagtt ccgcataact
     4801 ttgcttaaac catagggtaa cactacacac catcagcatc tgggttactc ggattactcg
     4861 gaattccgca tactgataat ttaatcagta cctctttccg aataatcgta gtccaacctg
     4921 gtcctttttt ctctgactcg tctgcattac tcagaaacaa acgttatgtc gtcttttttg
     4981 gcatgaacga attcatactg cagagttcga tccagacctt gcgacagcgt atacggtgcc
     5041 acaaaacctg aagaatgcac tttcgttgcg tcaaactgtg ttgttgcgca gaattttttc
     5101 acgcgcactg agctgacagc gtattttttg cccgtaattt tgctcaggat atcaaaacaa
     5161 tatccaccca gcattcctag tgggtaaggc aagtgcatag aagggatctt tttgttcagg
     5221 ctttgttcaa cttcagcaac caactggttc atgttcaggt ctggcttatc aacgtagtta
     5281 taaacctcat aacctgcggc aacattcttc agtttgtact tgataaactc aacaatgttt
     5341 ccaacataag ccatggactt atagttagtt cctgcaccca ccatcataaa cttgccgcca
     5401 gcgatctgtt tcagcaagtt atagacgtta ccgcggttgc gttcaccgaa gataacggta
     5461 ggacggatga tagttaatga acgttctgtt ggtgctttgt tataccattc acgcagcact
     5521 tcctctgcct gccacttact tttgccgtag tggttgaaag gatcgtgtgg atggttttcg
     5581 tcagggttgt gtttgttcaa accataaaca gcaacggaac tggtaaagat gatattttta
     5641 acgccatttt tttccatggc cgccagcaca ttgcgggtac cctgaacgtt gacatcataa
     5701 tagagagaag tagggctgac gtcatcgcgg tgttccgctg ccagtagcac aacagtgtca
     5761 aaaccggcta acgcctggtc gagtgcctgt tgatcacgaa catcgccaat ctgtgtgatt
     5821 tctggataaa agtggctctg ctgtttgtcc aggttcttga tattaaagtc agcaattgcc
     5881 gtttcaagta gtcgggttcc tacgaatccg gaagctccta tgagcaaaac gttatcgttc
     5941 ataaatcact ttagtctggt tgttacgtaa gaaacacaag ataaagatga gtaccttccc
     6001 tgagtagtca atgctgccca gccccagctt taacagttag tgtgaggatt ataatctttt
     6061 agaacattat atccagtaag tttatgaatg gtcgcaaatc tactccctcc gttccggcaa
     6121 tctaaagtta atcttttcca cattaacaat atggtgattt atcctgtcga tatcgacgga
     6181 gctttgccct ctttcattca ccgcatgaac atttgcaaga gacaggagtg tttctttttt
     6241 cgccataaag acgccgcgaa cgtctttgcg catgtcgaag ttcatgctca atgctggtcc
     6301 aactgaggat tcctgcatca ccttgatatt acgcataaaa agatgttgcg gtttgttgtg
     6361 taactccagc gacgcacgct tcatctcaat gttagtcagc gccacaaagg agacagcatt
     6421 cccggcggag atttggatgc cgcgcaattt ataagcaaga tgggtgttat ccagttgaat
     6481 attattcact cggaaatttt gcggtatcga gagatatttg cctttaatta ccccatagcc
     6541 gattaacatc ccggcgctat taatcatttc aatattatca atcacgaaat tgtcacaacc
     6601 gtaaatagcg accgcggcgt tatcaatgcc cgctttctta ctgaaatccg gcgtgatatt
     6661 gcgggctttg atattactaa taacaaaatg tttgccattt tcaacatgga tcaactgccg
     6721 acaatccgat cccgtgatat tcgccacgac aaagtttttc actgcctggt cttccgggta
     6781 gttgttatcg taggtacttc ccgcaagacc tatgccaatg ccccagttga ttttgccgtt
     6841 agtacagttg atgcgctcga tgacatggtc agatatcaaa atatcactgt cgttaattgc
     6901 cacgttccat tcaatggcgt cgccttgtaa gtcactgaac ttacaattgg tgatgttggc
     6961 accgattatc tggttatgaa atccctggcg caaaatagcg taattagcgt ggttaacggt
     7021 gagattatcg atggtcaggt tgcgcatgac ccgtttgttt ttgccgccga tataaatctg
     7081 cgttaccggg ccaaagccgc tcatcgccag ccctttgatg gtgcagtcag agccacgcac
     7141 atccagggtg atgttatgca tactgccgcc ctcctcccct gtcacctggc tgccgtcctg
     7201 taagataaat cgccctctgc cgttgccgcg caggcttccc aagatgtgta acgttttacc
     7261 tgcaggaatg aagatgccgg tgttgatatt gtcacaaacc aatccggcag gcacgacgac
     7321 cgtttgccct tcgctgaagg cttgtttaaa tgaggcgatc cagtcgtgtg ggttgtagtc
     7381 gttaatgtta acgctttgtc gggcgggaag tgcgcgagcg aaaggggtat ggaggaaggc
     7441 aagcgccgag cttgccgcca ggaaggtgcg tcgggagagt tttttaaatg gcatgcgttc
     7501 tcctctataa agcctgcagc aagctggcga gttctcgatt aatcacctgc tggttaaaat
     7561 cgtgttcgac tttttcgcgc gcacgtttga caaccggagc cagttcgtcg gtgtccagtt
     7621 ggctaaacgc agccagtcgt tgcgccagtg cgcgagcatc gttctcaggc accagccagc
     7681 cggatttatc ggcctccacc aattccggta ttccgctatg cagagtagaa accaccggaa
     7741 tgccgaccgc catcgcttcc atcagcgcta ccggaatgcc ttccatatcg ccatccgcac
     7801 ccgttaccga tggcaacagg aagacatccg cgtcgtcgag catcgctttc acttcatggc
     7861 tcggtttaaa gcccggcatc tccaccacat cttccagttg atattgttcg atgagggtgc
     7921 gcaggcgtcg ttcccacggg ccaatgccga ggatgcgata gcgaaatgcc acgccctgct
     7981 ctttcaactg ccgacaggct tcaatcgcca catgcaggcc ttttttctcg gttaagcgtg
     8041 cgacggaaat gatttccagc ggcgttgcag gcgctttcac cggacgcggg ctaaagcgcg
     8101 tcatgtccac gcccatgcgc gatacggcga ttttttccct cgggcagccc attttttgca
     8161 gccttccggc ccacagatcg cttatcggta gcatcaggtc gccacggcgg aacagttgct
     8221 gatattcggg agtgtagtgg ttgagcactt cccgacttga gatatcaatg ccgtggaaga
     8281 tagtagcaat tttgccgcga atgacaccca gttcgcggag ttttgctgct gttacccccg
     8341 ctggaccaaa atgagcgata aagacatcgg catgaaatgg tgttgcgacc tgaccgcaaa
     8401 tggcagacaa aatcaggttc cgcgactctg cgccatagcg tttgaggttg agcgcctgcc
     8461 aggtattttt acgatgaatg ccgcgtaagg tctggctggc gcggtggtgc agtttcgcca
     8521 ctttgcccgt aggttcgtcc tgtaaccagc gggttctggc ggcaaggttg tatttcgtcc
     8581 atgccgcgtg ggtgttttgt gtgtcgcctt tttgcagcgc gacaatctcc acctcaaatc
     8641 ccatatcaat aaacgcggta atttgattga ggacgaaggt ttctgacgac aacggaaatt
     8701 tcagtaaaaa gaagccgacc ttcatttcac ctccccgatg cgctcaagca cagactgcac
     8761 catctgcatt cccgtctgac gctcacgact gacagcttcg ttaagtcgtg cgttcagcgc
     8821 cggaagctgg cctaaggtat ccgcaaccat cgcttgcagg ctgccgtcca gtaaatgacg
     8881 gatatcaatc gccatctctg gcagtcccag ctgctgcata atcccggcgg atttatgttc
     8941 atagttgatg gcaatcgccg gagtggcaaa gttcattgag ataatggcgg agtgcaggcg
     9001 cgtaccgacg gtgagtttac aggctccgag aattttgccc atttccagat cgttaagttc
     9061 atccatcact acgtggtaac gggcaggatc gctgatgtgc tggcgtagat tgagcgccac
     9121 catgcgatcg tctttgttat agctgtcgat gcccgtacag gtggaaagcg caatcacctg
     9181 atacccttcg tcgagaatgc gattgaccac cccggcaaag gctttttcat acgcttgttg
     9241 agtggtgccg agacgtttgt cgaacggtgc cagttcgcgc agggtaattg ctaccgtttt
     9301 ctgttgtgcg gcaacgtcca gccagtgttg gacggcatag ctggcggtga agtcttctgt
     9361 gtggtgatcg accagccacg cggtatcgac gccatgttcc acttttgcgg tagtgatatt
     9421 gctgcgtttc atcaaatcaa ggctgaccga ttcgcgcagg atcagcgcgt cgcagtgacc
     9481 aaagacatag ttcgctagtt ggttaaattg ctcatcctgg aacgggccaa cactgtgacc
     9541 aatcataaat agcggctttt tcgccataaa cgtgcaaagc gcatgttcaa actgcggcac
     9601 gccgtagaga tcgacaaaaa acgatccgcc gacctgaata atggcgtcgt aacctgacag
     9661 caggcgcacg aagtcggtga atccctgggc gatggcgata ttacgcagct tgccagtgtc
     9721 ggtgacgcgt gagagcaata cctgatgctg gtagcggcga cggaggactt ttttaacgcg
     9781 cccaacaacg cccgccgcgc tgttgtgttg tttcatttgc aggaacagcg gatcgcccat
     9841 taccgggcgg ttgagcagcc aggaagaact gaccggatag cggctcatca cgtccacttc
     9901 ggcatgtgga ttgaggatgt tgatggcatc aagtaagccg cgcaagatgg cgctgtcgcc
     9961 acgattgccg caagtgtggt tgcccagaat aagtaatttc attttttcct cataaatttg
    10021 atgccaggtg aggctgtgtt tgttttgttc gttgccgctt tttgcctgat gcgacgctga
    10081 cgcgtcttat caggcctaca agacccgagc acagaaccgt aggccggata aggcgttcac
    10141 gccgcatccg gcattcagtg cctgatgcga cgctgacgcg tcttatcagg cctacaggtc
    10201 ccgagcacag aaccgtagga tggataaggc gttttacgct gcacccggca accgttgtcg
    10261 gaaccgaaaa caacaactca ccccacccgt aaaagcattt tcattttttc gctgcgacaa
    10321 aactgacgct tcacttccac caccagcgga tggcgcgaca gcacaatcat caccacaaac
    10381 gccagcacac ccgcggctat ttgcaccgcc agcagcatcc ccagcgccag ttgccctttc
    10441 agcacaatgc ccagcgcaga actgaccacc agcgtcggca gcgagagata aaacggcagc
    10501 cacaaactca ggatgtactg gcgataactg gaaccgagca ccggtttaat catcacgaaa
    10561 tagctcagaa tggtgttgat aatctgcacc agcaggaagc caagcgtgac gccgattgcg
    10621 cccgccatct gcccaccaat aacaatcgcc ggaataaaca gaaatgtttt gaatacgttg
    10681 aatttaaagc tgatgtccac ccgtgctttc gccatcagca gcgaaccaat cgggttaccc
    10741 acggagcgca gtagccccac cacacacagc aattgcagca ccggaataat gctgttccac
    10801 ttctcaccaa agaccagcgg tacaaagtta ttcgacacca ccatcagccc cagtagcgcc
    10861 ggaaagttga taatccccac taccgacaac agcttgtaga agttaacgcg cagcttttcg
    10921 gtatcgtcct gaattttggc gaatgccgga aacaacacgc gggtgatgat cgggttcagc
    10981 ttcatcggcg gcacaacggc cacgttgtac gccaggttgt atccccctgc cacgcccgcg
    11041 ccgagaatac gcgccagcac cagcgttgaa aggttggtgt tgagatagtt gatgatgctg
    11101 tccgccgtca gccaggcacc aaagcgtaag ttcggcgcaa cagacgccag cgagaaatgc
    11161 agaccggggc gataaatttt ccggccaaag tagccaaaca gcagcgttct caccgcacta
    11221 ttgaccagat aaccgaggat cgcggtcatc gccagcggcc agaaatgggc gctaaccact
    11281 gtaaaagtga agcccgccag caccgcgctg gtttcgatca tgccgatttt gttgaactcc
    11341 agctcttttt gcatcaacgc gcggaactgt tgcccgtggg ggatcaccac aaacgccagc
    11401 gacaatgttt taatcaacgg tgccaggtcc gggttattca gcacgtcgcc gatgagatca
    11461 ctcaacaaaa acaccaccac acacaccaca agccccagcc caacgttcag ccagtacagc
    11521 gtggtgagtt caaggtggct gatttctttt cgctgaataa tcgagttagc aataccgaag
    11581 tcagaaagcg tatctgccag cgcgatgatc accagcgaca cggtaagcaa accgaactgg
    11641 tggttgtcga taatccgcgc cagcacggtc atctgcacca gcccgaggcc gatgatgatc
    11701 accgtggcaa tcgccgacca cttcgcgccg ctgatggttt tttcacgtaa gctcatatca
    11761 atatgccgct ttgttaacga agcctttgaa taccgtcagg aaaacgattt tgatatcgaa
    11821 ccagacgctc cattcgcgga tgtactcaag gtcgaactcg acgcgttttt ccattttctc
    11881 cagcgtgtcg gtttcgccgc gccagccatt aatctgcgcc cagccggtaa tgcccggttt
    11941 caccttatgg cgcagcatgt agccttcaat gagctggcga tactgttcgt tatgcgctac
    12001 tgcgtgcgga cgtggaccga caatcgacat ccccccggtc agcacattga taaactgcgg
    12061 cagttcatcc agcgaggtgc ggcgcagaaa gttccccact ttggtgacgc gcggatcgtt
    12121 ctgcgtcgcc tgggtcacca ctttgtcgtt ctccatcact ttcatcgaac ggaacttcca
    12181 cactttgatc ggtttgccat ccatgccata gcgagtctgg cggaaaataa ccggccctgg
    12241 tgaactgagt ttcaccgcca gcgcaataca gcacagcacc ggggagatca gcaacagaat
    12301 aagcgtcgcc agcacaatgt cttccgcacg tttgagcagg cggttaaccc cggaaagcgg
    12361 cgtgtcgtaa agcggcacca ccggaacgcc gttcatctct tcgagacgtg aatggagaat
    12421 gttgaaggta aagacatcgg ggatcagcag caccgaacag gtggtgtccg ccaattgatg
    12481 gaccagtttt ttcactcgcg cgccgtcgca catttgcatc gcgatataga cgttatgaat
    12541 cttgcccgct ttagcatcct cgaccagctg ttgcaggtta cccgcccagt cgttagaaac
    12601 gccgcccggt ttcgggtcgt ggtaaacgcc caccacttca aaccctaacc acggctgatt
    12661 acggaagctc tccatcagca tttgtccggc ggctaaatcc cccgccaccg cgaccatgcg
    12721 cttgttatag ccatgattac gcagccagcc cgccccaata cgaatacatg aacggcaaac
    12781 caccagtccg atgctggtca gcccatacca cgccagccag attttcaatt gcgtgtcgaa
    12841 atcattgttg aacgccacca gtccggcgct gaaaatcacg cttaacgtcc agttttgtag
    12901 caacagggca aattctgtcg ctgcccgaac accgcgccat gagcgataaa aatcggtgat
    12961 gccgcccagc atctggaaca ccaccagcgt aatcagcgcc accaacaggt gcatgtagag
    13021 gaatgacagt ccgctcactt cgcaaaccag ccatagcccg gcaaacatga tggtgatatc
    13081 tgaaaagcgt tgcaccatag agattaacga tgcattggtt ttcgctcgct cgcgcttttt
    13141 tagatttgtc atcgttgttc ctgtttttag ctccttaccc gcagtggggt aagggaagat
    13201 ccgacattac tcgttcagca acgtcagcag agttcgcgtt cgcgcttcca tcagcggcac
    13261 atcaccgcgt gattccacat tcaaccgcac caccggttcg gtgttggagg tgcgcagatt
    13321 aaagcgccag tcggcaaagg tcatgctgat gccatcggta cgatccaccg ccagcgcctc
    13381 gcggctaaag tgctgttcca cgcggttaat cgcctcaaca ggttgtgcca gtttgctgtt
    13441 gatctcaccg cttgccggaa acgctgacat ccggtcgcgc accagttcgc ccagcgtttt
    13501 tcctttcagg cacaccagtt cggcgaccag cagccacggg atcatgccgc tgtcgcagta
    13561 agcgaaatca cggaagtaat ggtgggcgct catttcgcca ccgtagatag cgtcttcctt
    13621 gcgcatacgt tctttaataa aggcgtgtcc ggttttcgac attaccgggg tgccgcctgc
    13681 ggccgtcacc acatcaatgg tgttccagga gagacgtgga tcgtggatga tcttcgcgcc
    13741 gggatttttt tcgaggaacg cttctgccag caggccgaca atgtagtagc cctcaataaa
    13801 ctgccctttt tcgtcaaaca ggaaacagcg gtcaaaatcg ccatcaaagg caatgcccat
    13861 atccgcgccg tgtttaatga ccgcattgcg ggtatcgtcg cgacattccg gcagcaacgg
    13921 gttaggaata ccgttgggga aattgccgtc cggcgtgtta tgcactttga tcaattccac
    13981 cggtgcgccg agggctttaa agcgggcttc aatggcgtcc accaccggac ccgccgcgcc
    14041 gttccctgag ttaatcacca gcttgagcgg cgtaaggttt ttgacgttga tataaccgaa
    14101 caggtgatca acgtaagcgt cacgcagatt gatttgctga tagcgaccgc gtttggtttc
    14161 atcgacggga ggaaagtcgt tgccttctgc cagacgctgg acgtcgcgca gtccggtatc
    14221 gccgctgatc gggcgagccc cttcgcgcac cagtttcatg ccgttgtaat ccatcgggtt
    14281 atggctggcg gtaacttcaa tgccgccatc cacgccgaga tggaacgtgg cgaaatagat
    14341 ttcttcggtg ccggacatac caatatctag cacgtcgacg cccgcatcct gtaaaccttt
    14401 cgccagcgcc agttttaagg tttcgctggt gaggcggaca tcaccgccta acacaatggt
    14461 tttcggtttg agaaattcgc cataggcgcg accaatgcgc caggcgatat cttcattcag
    14521 ttcttcgcct aattttccgc gaatatcata ggctttaaag caggttaatt ttttcatatc
    14581 gttacccttt ttcaggcaat agttggccct gaccgaaacg gccaattttg tttttgtcgt
    14641 tatttacatc gcggtttcgc attcattgcc tgatgcgacg ctgccgcgtc ttatcaggcc
    14701 tacaaacccg aaccgtaggt cggataaggc gttcacgccg catccgacaa acagcgcctg
    14761 atgcgacgtt tacacccgtc cgtagcgatc cgcgaagcgc accacatcat cctcttcgag
    14821 ataagagccg gagcgcactt caattaaatc gagcggaatt ttccccgggt tttccaggca
    14881 atgcgtcgcc cccagcggaa tataaatgga ctcgttttca ccaagcagtt tgatatcacc
    14941 gtcgatagtg acttttgccg ttcccgcgac aaccacccag tgttccgcgc ggtgatgatg
    15001 catctgtacc gacaaccctt cgcccggctt cacggtgatg cgtttcacct ggtagcggtc
    15061 gcccgcgtcg atagagtcat atttgcccca cggacgatac acttcgcgat gcacccgatg
    15121 ctcatggcga ccatcagctt tgatctgctc gaccactttt ttcacatcct gcaccgcgtt
    15181 acggtcggca atcagcactg catctttggt ctgcactacc accaaatctt tcaccccgac
    15241 ggtggtgacc aggccagatt cggcatacac atagctgttt tcagttttgt gattaatcac
    15301 atcgccatgg caaacgttgc cctcggcggt gtgggcactg atctcccata aagaggacca
    15361 ggaaccgaca tcgctccagc ccgcatccat cggcaccaca acggcatctg ccgtgcgttc
    15421 catgaccgcg taatctaccg actcttccgg acaggcgaga aacgcctctt catccacacg
    15481 aataaaatcg agatccggat cgacggcgct catcgctttt tcacaggcat cgagaatatc
    15541 cggacgatac tttttcagtt cttcgagata acgtccggca cggaacagga acataccgct
    15601 gttccagtaa tattcgccac ttgccacata ggcctgcgcg gtttccagat tcggtttttc
    15661 gacaaactgc gccacttcaa aggccaccgt atcctgctca cccgccgaca cttcaccgcg
    15721 acgaatatag ccatatccgg tttcaggtag atccggcaca atgccgaagg tcaccagctt
    15781 gcccgcttcg gcatacggca tggcattacg cacggcggca cggaacgcgt cttcatcggc
    15841 aatcacatga tccgccgcca gcaccagcat taatgggtcg ttctccgggc tatgacgttt
    15901 tgccgccagc gccgccagcg caatggccgg tgcagtgtta cgccctgccg gttcgagaat
    15961 aatgttctcg gtgagtttgt tcagttgacg cagctgttcc gcgacaataa agcggtgctg
    16021 ctcattgcaa atcaccaccg ggctttcgca ctccacgccg ttcaggcggc agatggtggt
    16081 ttgcagcatg gtgagatcgc ctttcaggca taaaaactgc ttggggtaaa gtacgcggga
    16141 aagcggccat aagcggctac cggagccacc tgccatcaca actggataga gtttcgactg
    16201 cgccataatt atccccgaat atcatttata aattgacgta acacgttctc tttatcgagc
    16261 gtgcgttcgg catattcacg tgccaccgtg ttgtgtttgg gcagcaggaa cgcctgacga
    16321 atccccgcca ccagcgcctc gaccgattcc ggttccacgc aaaccgcaat gcccggaaag
    16381 gtttcgcaaa gctgccccag ttctgtgtgg gcttctgcgg taatcaccgc gttaccgcct
    16441 accgccagaa tattggtcag tttcgacggc aatacggcat ctgccgcgcc gcgtttttgc
    16501 accaccagat ggcaatcccc catcttcagc agtgcgggta aagcgtcata cgattgcagc
    16561 ggaaaaaatt gcatgttgcg cagtccacgc tgctgcgcca ttttttccag ccgcgctttg
    16621 ccgccgccct gcccgacaat ggcaaaaata agcggttcat cgcgcaggcg atcggcagct
    16681 tcaataacgt tttccagccc ctgcttttca ccaatattgc cggagtaaag aatgattttt
    16741 ttgttatccg gcaggccaag ctggttacga agggcatcaa catcggcgtc tgcgacatgc
    16801 tgaaaacggg caatttctga ccagttgggg aagaagatga cgttttccgc cgccacgcct
    16861 ttctcgatag ctttattcat catcgaacgc gaaatcgtgg agacgttatc gacgttatgc
    16921 agtccgctac gttcgaacgc cgttgccagc tgtgccactt tgccgccttt accttttccg
    16981 gcaaggccca gccccagcat ggcgtccact tcgtaatcct gaatatgcag cacggtacgc
    17041 gcaccagaga gtttcgccag caggcgcatt cccggcgtgc aaaacagcgt tggcaccacg
    17101 ccaataatgc gatccggctt ccagcgacgt tgcgccatca acgggaaaaa actgctgacg
    17161 gcaaaactgc cgagatgcaa caggcgtttc agggtgctcg gctgttttgg cacgtacagc
    17221 gggcagcgcc acaccgtggc ggccccctct tcccgtttgt accgccaggc ggaatagttc
    17281 tcgcccacct gccactgcgg gtagtaaggc ggcgcggtaa taacccgcac ctcatgacct
    17341 tgtgctgcca gccattccac catctcgccg gtgtacttgc cgatgccggt taactccggc
    17401 gagtagttaa tgccgtagac cagtattttc ataatccggg tactccggca cgcttctcag
    17461 cgagaaaata ggcgcggctg ttagcgtgaa cattgtcact ggcgagcagt gcgtccgacg
    17521 tcagccagcg gtaatcgtca tgctgctcat ccggcagtaa cagttcttct tccgctactc
    17581 tgaagcgaaa accgagcacc acatagtgag tggtgaaatc cgtgccagag aagttatcgt
    17641 cataaaagtg ctgccagaca ccgtaaaact ggcctgctgt tatcggcaga cgcagcccca
    17701 gttccgccat cgtcagccgc tcaaatgcgg cttccagcgt ttcgtctttc tgcacgcgcc
    17761 ctcccggcac aaaccagtaa ccctgcgccg ggcggttggt tcttttgcca agcagaaact
    17821 cgccgcgact gttctcgaca ataaagtcga gagagacaag cggagtggag cgcactaccg
    17881 tggcaaagtc ttcctgacgt aaaaacatca ttacccccga aagcggtctt gattctcaag
    17941 gaaccactgg taagtgctgg caagccccgc ttccagtgag atttcgtgat accagccaag
    18001 ctgatgcagc cgcgtcacat ccagcagttt gcgcggcgtg ccatccggtt tgctggcatc
    18061 aaaaaccacc cgacctttgt aacccaccac tttggcgatg gtttgcgcca gctcgcggat
    18121 agtgcagtca acgcccgtgc cgacgttaat gtgcgacagc atcggctggg tgttctcctg
    18181 ccagacttca tgcgccagct ccatcacatg aatgcttgcc gccgccatat catcgacgtg
    18241 cagaaattcg cgcatcggcg taccgctgcc ccacaccacc acgtccggcg cattctgtgc
    18301 cgtcgcctcg tggaagcgac gcagcaatgc agggatcaca tgcgaattac tcggatggaa
    18361 gttgtcgtgc ggcccgtaca ggttggtcgg catgactgag cggtaatccc gtccgtactg
    18421 gcggttgtaa gattcacaca gtttgatccc ggcgattttg gcaatggcat aaggctcgtt
    18481 ggtcggctcc agcgtgccct gcaacaactc gctttctacc atcggctgtt ttgccagttt
    18541 cgggtagatg caggacgatc cgagaaacag cagtttgttc acgtcgttct gatgcgcggc
    18601 ttgaatgata ttgctctcaa tcatcatgtt ctgatagatg aaatccgccg gatacgtgtt
    18661 gttggcaaca atgccgccca ctttcgccgc cgccagatag acctggtcaa tacgttcgct
    18721 ggcaaagaaa tcatgcacgg cgcggctgtc cagcaggttc agttcatcgc gggtgcgtaa
    18781 taccagctct acatcaccgc gctgttcgag ctgccgcctg atggcggaac cgaccatccc
    18841 gcgatggcca gcaataaaaa tgcgttgttt actcatgctt atgactccag cgcgatcgcc
    18901 acgtcgtagc cgtgagattt cagcagagag tgttttttcg ccgcttcaag gtcattagcc
    18961 accatttcag acaccatctc tctgagggtg atttccggtt tccagcccag tttttcgtgc
    19021 gctttggtcg ggtcgccgag cagcgtttca acttcagccg gacggaagta gcgcgggtca
    19081 acggcgataa tcacatcacc cggtttaacg cccggcgcgt catgcccggt gacggaaacc
    19141 acaatgccct tctcttcaac gcccgtgcct tcaaagcgca gtttgatgcc cagctgtgcc
    19201 gccgccattt ccacgaactg acgcacggag tactgaacgc cggtggcaat aacgaaatct
    19261 tccggttgtt cctgttgcag catcatccac tgcattttta cgtagtcttt ggcgtggccc
    19321 cagtcacgca gggaatccat attgccgaga tacaggcacg actccagccc ctgggcgata
    19381 ttggcgattg cgcgggtgat tttgcgagtg acgaaggttt cgccacggcg aggggattca
    19441 tggttaaaga gaataccgtt gcaggcgtac atgccgtagg attcgcggta gttaacggtg
    19501 atccagtagg cgtacagttt ggcgaccgca tacggagagc gtggatagaa cggcgtggtt
    19561 tctttctgcg gaatttcctg caccagacca tacagttcag aggtggaagc ctgatagaaa
    19621 cgggttttct tttccagacc gaggaagcgg atcgcctcca gcagacgcag cgtacccatc
    19681 gcatcaacgt ctgcggtata ttccggtgac tcaaaagaaa ccgcaacgtg gctcattgcg
    19741 cccaggttgt acacttcatc cggctgcact tcacgcaaaa tgcgcgtcag gttggaggta
    19801 tcactcaggt cgccataatg cagatggaat ttcgggttgc aggtgtgcgg atcctgataa
    19861 atgtgatcca cgcgctcggt gttgaatgac gatgcacgac gcttaatacc atgcacctcg
    19921 taaccttttt ccagcagaaa ctctgccagg taagaaccgt cttgtccggt tacaccggtg
    19981 atgagagcga cttttgacat gtcttattcc tctgtatttt ttgaatttat tcagtttcaa
    20041 cgcgttcgcg tatcaccact gcgggattcc cccggcaaac cacatttgcc ggaagcgatt
    20101 taaaaacact gcttcgcgca cccacgacgg tgccgttgcc gatcatgacg cctggggcaa
    20161 caaagacatc ggttgccagc cagcatttct cgccaatcac aataggcgtg gcgttaatgg
    20221 tgaaatgttg acttgcatgg tcgtggctac cggtgcataa ataacttttt tgcgatatca
    20281 ccgaatttgc gccaatggtt atttcaccga gggtatataa attgacgtca tcgccgaccc
    20341 acgcgtaatc acctaaggtt aatttccacg gataggtaat ttttactgac ggacgaatca
    20401 ctacgttttt tcctattttt gcgccgaata aacgtaataa aaatgcccgc cagcgataca
    20461 atacttgtgg cgaccaggca aatattgttg cctgtactgc ccaccataat tgcactttaa
    20521 tagcgttgcc accccggaac cctttcggca ccgagaatcc acttaaatcc tgcatcgttt
    20581 tcccttatat tcagcttttg ttatataagg ctttcgtctt tgaggtcgta cgttggcgta
    20641 aatgccagga taattcagcc caaaagccag gcacgtgcaa tatctgccgt tggacttttt
    20701 ttgcgtcagc acacaattcc atattattgg tggttgatac cccacccata gaaaattcag
    20761 acaccaggcc attgagtttt ttaaatgcat aaccggctct atacattttg gccgccagcg
    20821 cgtagtcgga agaaacttta tattccagat cataacgcca tttcttcaag ccggacaccg
    20881 ggaaaaatat cgcctgatga ctggcgggca gactgtggta aatataccaa cccggtttgg
    20941 cgctacgttt aattttatgc ccgtcgccaa aatccagcag cgcatcgccg gtgatcatca
    21001 tgttatcttt ttgcactttt aacttacgga caaaatttgc ggcatcctga tgaaaaatat
    21061 cgcctgagtt gagaaacaac gcgaacttgc cttgcgccat cgcaatgcct ttgttcatag
    21121 cgtcgtagat accgttatct ggctcgctga caaagcgtag gttatagata ccattgagat
    21181 tttccagata ctcacgggtg ccgtcattgg aaccgccatc gacaacaatc cattcgaagc
    21241 tgatatcgtc cgcctgcgcc agatgcgcca gcgaggcatg tgttttgact atcccttcga
    21301 ggttacgaaa cgcgacagtg attatgctaa gcaacatgtt cttattctta cctcgtaata
    21361 tttaacgctt ttcgcaaaat aaacggacag acgattaaaa atgcatattc cgggctaaat
    21421 atcgaaccgg taaaaaacag cgataccgga gtaaaaagat ataattgcac gcgaaaattg
    21481 cggttatcac caaaagcgtt gatcatcatt tttatcactt tccccatgta ccacagtgat
    21541 aaaaacaccg cgaaccagga aaaataaata atcagcagat acaaaccatt gtctatggtt
    21601 tttccgacat ccgcaccgtt aaatattccg aatgatgcga catattcata aagtgagcca
    21661 aatctgacta caccgtcaat atgggttaaa gaataaccga ccatcaccag cggaccgaca
    21721 atacgataat acgatgacga tccttctgta cctaaatcgc ccagacgggt ggaaatataa
    21781 gggaacgcga ttaccacacc aaccaggaat acagccagag aaatcaatgc taacggtaac
    21841 tttgtcttaa tcgcctcttc attcagatat tgaaatgccc actccagcaa ataaaaaagg
    21901 ataaaggtca taactcctga aaacgatccg gataatatta tccctgcaag aatcatagca
    21961 tcggttttag gcgttttgat accaaactgt ttgatgctga gccaaattga gattaatgcc
    22021 agagcgaaaa atgccggttc gaaataaagt gctgtcgtgc gcttgccgcc gaatttaatg
    22081 aaattcagca catagctgtt gctgtaaatc agatatttcg aaatcgactc cataatactg
    22141 ctaccgccgg tgaggataat ttgcgccatc tccaccgccg ccagcaccac caccagcccg
    22201 accaccagat aaaagaaacg taatatcttg cgatggttgt gtggcgaaat cgttttaaag
    22261 cgaatactcc acaccatgcc aataatgatc acaatataga caaacagcat ggttgaagtg
    22321 acatatttac tggcatccaa cgactgacca aacaggtagt taaacgccgt gagccccgcg
    22381 ccaatcccta aggcaatcat caatttttta acgctgatgc gctctaaaaa cagcagtaac
    22441 aagacaggta aaaaagtgac gatggtgatg gggaaacttt cgccaagctg ggcgattttg
    22501 acgttaacca gcaggtagat cagcggcagc agcaggtagc tacagattct gatagaagtt
    22561 gacatactcc tccagcatct gttgtccact gtaggcggcg cggctgcgct ggctgaactc
    22621 agccagcgtg gtaccaaata tcgcctgtgc gatttccggt ttgcttaact gcaccagttg
    22681 cagcacatct tcttcgctga cggttttacc accggatttt tgcagcactt cccgcgccgc
    22741 atcgctatgg gtggcgatca ccggcacgcc aatcgataac gcctcacaca aaatcagcgg
    22801 gtagttatcg acgcgagaac tgaataccag cgcatccatc tggttgagcg cgctcatcaa
    22861 cttgcgcttg tcagtttcaa agccgtgatt aacaacgtta ccagcggtga acggcgagaa
    22921 cttaccgaaa gtatgcagtt cgattttgtc acccagcgcc atcatctcgc gcaccagttg
    22981 ctggttagtt ttgccgtcgt aacgcaggtc atgcgccacc accgcgattt tcgacttgcc
    23041 ctgggtttcg cacaccggag gcaagtccgc cagaatcgct tcggttgcca tatcaatacc
    23101 gttattgata atccggcaac gccctggacc gtacagacta ttgaaagcat cagccacatg
    23161 ctggctggga gaaataaact gacagcccag cgccagcatc tcgcgaaata actgccgttt
    23221 gcccgccacc agttggtgtg cgcgatcaat cttcaccggc ggataattat ttaaggtcgg
    23281 gcatttctgg cagcccgttt tccagccttc gcaaccgtcg gtaaaggcgc agcgtccggt
    23341 aacgctccag tggtcgtgca gtgtccagac cagagtgaca tccggtttat ggtttttcac
    23401 tttttcacaa aagcgcacca cgctcttaag attcaaccag tagctgtgca gcacatgaaa
    23461 atgcaggacc accggacccg gtgtgcgagt aatggtgcga tataactcat tgaaattgcc
    23521 gaacagatcg cgattaaaca gacgaaacaa cgcaatgttc gccattgcgg tcatccgtgg
    23581 cgtatgtttg atgacctgcg gatagttctg atcgctgacg ctctctttgc cgcctttgcc
    23641 gtagccgtag acaaaatgtg acgccagccc ctgttgcagc gcgcgctggt ggagatctaa
    23701 cgccacacct gctgccccgc cttccgccag tcgcacatta aattgcagaa tattcattta
    23761 attaccttca ctcgcgcttt ttctcccacc accagcgcgt tgtccgggac agagtcgagc
    23821 accacgctgc ccgcgcccac ggtgacgttg ttaccaagcg tgatatcacc aagaataatg
    23881 acgttggcac cgagttcgac gccgttgcca atgtgtggac atgccatgtt atcggcacca
    23941 cgattgccga tagtgacgcc gtggcgaatg gtgaaatcat cccccgctac cacgttttta
    24001 ttgatcacta cggcgtaacc gtgatggata gtaaagcggc ggcctggatt tcataaccga
    24061 aaaagcattc ggtgataatg cgatacagca ccagcagcgg ggccgcccac agattgttga
    24121 ggacgttctt tttgcgccac accgaacaaa aatgagcgac gcgataggcg agaaccatgc
    24181 agcacgggcg taaactccag ctgttggcgc gcagatcttc cagcatcttt agcgcccccg
    24241 aataccatca gccagacgtt tgccgttacg caccgacagc agcgtcagca aagtgcgcca
    24301 ggtcatgcgt ttattgcgga tctggtagag ggtaaacagc tgatattttt tgctggcgcg
    24361 gtcgaatttg tctttgtgct tgcggtaaaa gtggaagtat ccggagaatt ttttcggtga
    24421 cgaggtgatc tgcatttcgc cgtgattgat atgcaggatc tgcgtcgcct cttccacttt
    24481 ccacggttcg ccgtactcca ccaccatccg caggaagatg tcgtaatcct gtgccgcttt
    24541 cagttcggta tcgaacaagc actctttgaa acgccacgcc caggtaaaga cctgattgcc
    24601 aatgatattg cgtttgtaga acaggcggcg tgaatacggc gatttgggat acagcggcaa
    24661 gcttgccggt tgggaataaa cttcgccctg gcaaacgtag tcgttagcgt acaaaaaggc
    24721 gtgtgtgacc agttgctgtt tatgggcgag gaagacgctc aggcggttgg gtatccattc
    24781 atcgtcgtca tcgatcccgg tgatgtattc cccttgcgcc agcataatcg cctggttacg
    24841 taccgcgcac gccccgctgt taatgtcgtt gtgaatgtaa gtgatacgcg gatcgttgag
    24901 ggcggtgacg tactgttgga gttgttccca ggaggtggag caatcatcca cgatgatcat
    24961 ctcccagttg ttatagtcct ggcgcaggac cgattttatc gcccgaatcg ccagttgttg
    25021 gcgattccag gtcggcatat agattgagat cagcggattg tctttcatag gttgttctcc
    25081 cggcttatga gctggtttgc ttgccggatg cggcgtgaac gccttatccg gcctacgggg
    25141 cggtgcgaat gcaggcgtcg ttatttcgca tccgacttat attcgtattc gtaatagcca
    25201 taatcctgat atgcgctggc gcggcggaag atggagttca gaatcacccc tttcaccgga
    25261 ataccgtttt gctcaaagcg gctcagactg gtttccactt ctttcaatgt gttgactgca
    25321 taacgcgcca ccatcaacgt ggttccgaca tgacgaccaa caattgccgc atcggtcact
    25381 gccagaatcg gcggcgtatc aatcaacacc aggtcgtagt ttttactcgc ccacttcacc
    25441 agttcggcaa agcgttcgct cattaacagt tcagaaggat ttggcggtac ctgaccgcgc
    25501 gggatcaggt caaatttggc aatagaggtc ggtttagcag cggtagtaat atcgccctgt
    25561 ccaatcagaa tttccgacag gccattaacg ttattagtgc ccaacagctc gtgggtgtag
    25621 cctttgcgca tatcgcagtc gatcaacaac acccgtttat tggtctggct aatcaccgcc
    25681 gccaggttgg cgcagacaaa ggttttaccg attgacgggc taaccccggt catcatcaac
    25741 acattgttct gtgcctgcat catcgcgaag tgcagactgg tacgcagact acggatggct
    25801 tcaatcgcca gatcggttgg attccccacc gccagtagct ggctctgttt atagcgttta
    25861 atccctttga tggttttgac gctatcgcgc gctttctgcc actccgacag cgggatgctg
    25921 gcataaacgc tgataccgtg ttcttccagc acctgcgggc tttcgatacc gcgattaaac
    25981 aacgagcgca gcagcacacc cacgatagag agcatcaggc caaggataat cgccccaagg
    26041 ataatcagcc ctttcttcgg tttcagtaca cctggctggg tgattgccgg gtcaacaatg
    26101 cgcacatcgc cgacggtgct ggcttcggtg attttcagct cctgctcttt attcagcagt
    26161 tgcatataga cctgctgacc agactcgaca tcgcgggtca gacgcacaat ttcctgctgg
    26221 gttttcggca tcgccgttac gcgaccatta agtttggctt tttcgtcttc cagcgcctgc
    26281 cgtttctcca gcagcgtgcg gtacgccggg tgaactttgg tgtacagctt ggagatttcc
    26341 gcctctttaa aggtcagttc gttcaactgc gcgtcgatgt tcaccatcga atcgagcacc
    26401 gctttcgctt ccagcggcag atcaactgaa tctttatcct gacggaaggc attcagtttg
    26461 ttttcggcaa catcaaggcg gttacgtact tccggtaact gttgcgcgag gaaagcgacg
    26521 cttttcgacg cttccgccga tttgcgctca atattttgtt cctgatagtt acgggcgatg
    26581 ctgttaagaa tgtcgcggat ctgttcgcga tcttcaccgg tataagtcag gctcaaaacg
    26641 cctgcgtctt ttccgttctc cgttaccgtc aggctgtttt gcagttggtt gatcatcccc
    26701 agcgtggagt atttggtgac ggtaaactca ctccccgggc tggcgtgaat agcttcaacc
    26761 atcagcgtga cgccttcttt tttcagcatc tggcccgctt gcccacgggc gctaaagccg
    26821 ccatcactgc tcagggtgta gtttttgtca tcaagcacat taagcgtaaa cacccgatcc
    26881 gccatctctt tcgggcggtt aaaggtggtc actttcaccg tctcgttctg acgtcccatc
    26941 aggcgatccc agcccgcacc gaaaatcggg aacgtgtttt tgctcaccgc aatatcgagg
    27001 tcgagatcgt ccaccgtttt accaagcacc agacgcgaac gaatcaactg gatctcggcg
    27061 tccgatgctg gcggtttgtt ggctaatgcc gaaccaatgt cctgcactaa cgaattgcca
    27121 ctgttttgct cgatttgtac cagtgcgtcg gcactataaa tcggcgtggc gaagaaagtg
    27181 taaaccacgg aacagagggc aaatacagcg gtgatgccaa tcacccacca gcgtgcttca
    27241 atgatggtgc cgaccaggcg accaatatcg atttcatcac tgcccgttac cggagcggca
    27301 tgttgtttta ctttttctgt cattcttata cctgctctgc gttcaatgcc tgcgcccact
    27361 ggcgggcaga ccgttcaagt aatgtgtaca ccgctgtaaa cgtttcccgg cttttgcgat
    27421 acggatcggg gatttcacat tcgttatccc agtgaccaaa cagcatcact ttgccgcgca
    27481 tctctggtgc catctcgcat aagcgttcga tatggcgctt ttccatggtc agaatcaggt
    27541 cgtagttgcg acacagacgg cggctgattt gccgtgcgca atgaccttcc agggacagtt
    27601 gatgttctgc ggcgacgctg atagcggtag gatcagcgcc cttaccgacc agcgcgccga
    27661 gtccagcgga ctccactttt agctccgggt gataacgttg cagtaagcgt tccgccgtcg
    27721 gggaacggca aatattgccg acacagacaa ctaagatgtt gttaaacatg acgattacca
    27781 gttatgaatg tcgctggctg tatccgtcat gtaacggaca ccgctaatag ttggcagcaa
    27841 ctgattgatc agacggttcc accgggatac cggggcggtg gtgacataca ccacgtcata
    27901 aggttgcagg cggaattctg tcgccattac cagcgacgtc gcatcggaca tatccagctg
    27961 gtagatattg gcaatcttgc cgttacgccc gctctcgcct ttcagcggac gaatgacaaa
    28021 gatgccgctg gcgttggagg tggtcatgtc gatgccttca gcattgccca gggcttcagt
    28081 cagggtcatg ccgctaaagt ccattttcag ggtgctctgt ttcttcactt cacccatcac
    28141 aaacactttc agatcatcat tacgtggcac gtaaagaata tcgccggggt aaagcagtcg
    28201 gttctgattg aggtcgccgt tttgcatcag cgcctgcaaa gaaatgcgct cttcacgacc
    28261 attgtgtgtt agcaccacgt tgcgccagtc agcggtgtcg gtcaggccac ctgcggcgtt
    28321 gatggcgtcg agaatggtca gtggcacgtt ggtaatcgcc tgttgaccgg atttattcac
    28381 ctgaccggag atataggctt tttgcgagcg gaaggcggcg atattaacgt ccacctgcgg
    28441 gtcagcgata tacgtcgcta agcgcccggt aatatcactg cggatttcag cgagcgtttt
    28501 ccccacgacg tgaaccttgc cgatatacgg gtagaacatg gtgccgtcag gctgtaccca
    28561 gttgccggtg tcgctggagc tgcggtactg accggctggt gtagtgagtt ccgggtgatc
    28621 ccagacggtg acattaagaa cgtcccccgg cccgacgcga tactggtaat tcgcgatctc
    28681 actttccagc gtcatattgg ggcgcgctac gttgggtcgt gggcgtaatt gatcaatcag
    28741 gcgcggggtt aacggataaa cattcaccat tttgtcgagg tcgaaatcag cgtcctgctg
    28801 tttgatgacg tctttcccca tcgtcgacat attgctgccc ggaagtactg tgcaaccgct
    28861 tatcaaggtt actgatacca ataatggcat caatttcatt ttggatttca tcattgttta
    28921 tttatcactt tggcagagta attatcctgt gcactattaa tagcaatgtc gccatgcaca
    28981 tttaccttgc agttaattga ataaaaattt aactggcatc agtcctaaaa aaattgattt
    29041 catccgcagg ctattgacag aataattcag actggtcttt caggcatcca gacacgctac
    29101 cgcccctggc tttttagcta ccaatacact gatttagttt aatttttcaa accctctctg
    29161 catacagtcg ttgatgagaa agggttattg cggaaattaa tttccgaata taaggtgaca
    29221 ttatggtaat tgaatattgg ctctccaata atgcaggggg aagtgttaca gctaacggaa
    29281 tagcaggcaa ggtaacaatt cggcaattgg ctatttttaa gaattatatt aagtgtcatt
    29341 caatatggtt tttaggagtt tctttaggtt gacaatattt aatatggcgt ctccacatgc
    29401 gatatttctt aaataacgct ttattattat cacttataat tcggggataa tgtgaggtta
    29461 taaccctcaa ataatatgag ataaatagtg ctgca
//
LOCUS       DAZFFE010000052        29356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFFE010000052 DAZFFE010000000
VERSION     DAZFFE010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.052"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            139..729
                     /locus_tag="ACHJ69_002504"
     CDS             139..729
                     /locus_tag="ACHJ69_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5015466.1"
                     /translation="MSKKLGFALSGIMLAMAAGTAFAANDMDGGQLNISGLVVDNTCE
                     ARVDGGNKDGLILLQTATVAEITDGVLDTTVGAKAKPFSITIDCSKANPAPGTTAKMT
                     FGSVFFGNSKGTLNNDMSINTPSDGVNTALHNIEGSTIKQVQVNNPGDVYSKTLDSTS
                     KSATYDFKASYVRADASKAATAGYVKTNSAYTITYQ"
     gene            838..1578
                     /locus_tag="ACHJ69_002505"
     CDS             838..1578
                     /locus_tag="ACHJ69_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO5015467.1"
                     /translation="MFFNTKHTAALCLASCMAFSSSAIADIVISGTRVVYKSEQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASS
                     ALPKDRESVFWFNVLEVPPKPDAAKAANQSLLQLAFRTRIKLFYRPEGLSGISSDAPL
                     ALKWSWATSEGKAALRVDNPTPYYVSFSSGDLEANGKRYPLDMTMIAPFSNDVIKVNG
                     MSGRTSSAKVHFFAINDFGGSIEGNANL"
     gene            1613..4201
                     /gene="htrE"
                     /locus_tag="ACHJ69_002506"
     CDS             1613..4201
                     /gene="htrE"
                     /locus_tag="ACHJ69_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFO5015468.1"
                     /translation="MTIKSTNHLTHIATFCALLYSNSALCAELVEYDHTFLMGKDASN
                     IDLSRYTEGNPTLPSIYDVSVYVNDQPIMSQSIAFAVIEGKKNAQACITQKNLLQFHI
                     SSPDKNSEKAILLKRDDDLGDCLNLAEMIPQSSIRYDVNDQRLDIDVPQAWIMKNYQN
                     YVDPSLWENGINAAMLSYNLNGYHSESPVRTNDSIYAAFNGGINLGAWRLRASGNYNW
                     MTNVHSDYDFQNRYLQRDLASLRSQLVIGESYTTGETFDSVSIRGIRLYSDSRMLPPV
                     LASFAPIIHGVANTNAKVTVMQNGYKIYETTVPPGAFAIDDLSPSGYGSDLIVTIEEA
                     DGTKRTFSQPFSSVVQMLRPGVGRWDISAGQVLKDSIQDEPNLFQASYYYGLNNYLTG
                     YTGIQLTDNNYTAGLLGLGMNTPVGAFSVDVTHSNVSIPDDKTYQGQSYRISWNKLFE
                     NTSTSLNIAAYRYSTQHYLGLNDALTLIDEVEHPEQDLEPKSMRNYSRMKNQVTVSIN
                     QPLKFEKKDYGSFYLSGSWSDYWASGQNSTNYSIGYSNSASWGSYSISAQRSLNEDGQ
                     TDDSIYLSFTIPIENLLGTEHRSSGFQSIDTQLNSDFKGNNQLNISSSGYSDTNRISY
                     SVNTGYMMNKSSDDLSYIGGYASYESPWGTLSGSASASSDNSRQFSLNTDGGFVLHSG
                     GLTFSNDSFSDSDTLAVIQAPGAKGARINYGNSTVDRWGYGVTSALSPYHENRIALDI
                     NDLENDVELKSTSTVAVPRQGAVVFADFETVQGQSAIMNIVRSDGKNIPFAADIYDEQ
                     NNIIGNVGQGGQAFVRGIGQEGNIRITWIEEGKPVSCFAHYQQNTTSEKIAQSIILNG
                     LRCQIQ"
     gene            4218..4784
                     /locus_tag="ACHJ69_002507"
     CDS             4218..4784
                     /locus_tag="ACHJ69_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5015469.1"
                     /translation="MIKITPHKITILMGLLLSPSVFATDVNVDFTATVKATTCNITLT
                     GTNVTDNGKDKYTLVIPSMGMDKIANKTAQSEANFKLVANGCSSGISWIDTTLTGNQS
                     GSSPALIIPLASDTTSTTSYIGMGFKRKATSGDTFLKPNSAEYIRWSASEISTDGLEM
                     TVALRETSVGKGVPGKFRALATFNFSYQ"
     gene            4796..5404
                     /gene="yadL"
                     /locus_tag="ACHJ69_002508"
     CDS             4796..5404
                     /gene="yadL"
                     /locus_tag="ACHJ69_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFO5015470.1"
                     /translation="MMILKKLCYGLSGSLFLASSLLTISSHAATDSIGLTVTTDVEMG
                     TCTSTLTDDGTTKISTINFGDVYISEINAETKIKTFKLQFKDCAGIPGKKAKIKLTTR
                     ALCEGNSNNGPGFANASTATAKAAAVAVEVWSTSTPGKNGAKQFSCVTPATEEVSIAN
                     ATGSNVVDYPMSAVLVVAKDKTVTNVTAGDFTAPATFTVTYN"
     gene            5431..6027
                     /locus_tag="ACHJ69_002509"
     CDS             5431..6027
                     /locus_tag="ACHJ69_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO5015471.1"
                     /translation="MRPTQRNLMKKSILILSLLFSATSPVVAGQDVDIVANIKNNTCQ
                     SGISNNGNIDLGVVGVGDFTGNISAENYHPGGKEFTITVQDCTLQGTGNVLNQLHINF
                     RALSGVMATGSSQIFANEDNSGAKNVGVVIFSIQDPANTFNVLSATGSSRSVYPVMSS
                     ALNNSSWKFSTRMQKIDPALNVTSGPLISHVLVDIYYE"
     gene            6053..7336
                     /locus_tag="ACHJ69_002510"
     CDS             6053..7336
                     /locus_tag="ACHJ69_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFO5015472.1"
                     /translation="MQHDNGVKMKTFFRYFLFLALCSCCYTASAGTDDDVGYIVGNNY
                     GVGPGDQKWRETGPNGDVTVKFRYGSGTNNLVFYKPTQLGPTGVSLKWAQLDSASGGG
                     FLYCNRSDNSSGAPMSIEHKMVDSGKSYGGHKLFKTSVPGLYYTLAISNIWSTYTYTD
                     INPSGMYIGDSTSQSFNWRGESEQTLYWSCNNANSSKKYWAVGGVMQTLTIEFYTDTD
                     FNPTTNQRVTLSRTDSYLYSFKAYNAGISIKSYFLKIDFDLTDIVLTNPTCFTAALSG
                     PSVNGSTVKMGDYSPAQIKNSATAVPFDITLQNCIRVRNIETKLKSNKVGSVSKELLA
                     NTLTGNDAAKGVGVLIEGLKNTKSAQMVLKPNNATSIYKDYETEDDTTGGIYPDKGNG
                     TSQPLHFQATLKQDGNIAIEPGDFKATSTFQVTYP"
     gene            7448..8242
                     /gene="panB"
                     /locus_tag="ACHJ69_002511"
     CDS             7448..8242
                     /gene="panB"
                     /locus_tag="ACHJ69_002511"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO5015473.1"
                     /translation="MKPTTIASLQKCKQDKKHFATITAYDYSFAKLFADEGINVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8254..9105
                     /gene="panC"
                     /locus_tag="ACHJ69_002512"
     CDS             8254..9105
                     /gene="panC"
                     /locus_tag="ACHJ69_002512"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO5015474.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDIVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRSDDIQIRDADTLLEISENSKRAVILVAAWLGDARLID
                     NKLVELA"
     gene            complement(9187..10071)
                     /gene="rpnC"
                     /locus_tag="ACHJ69_002513"
     CDS             complement(9187..10071)
                     /gene="rpnC"
                     /locus_tag="ACHJ69_002513"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO5015475.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVVRDRETGGESMMTLAQWFEEKGIQQGRQEVSQEFALRLLSKGMSRE
                     DVAEMVNLPLAEIDKMIN"
     gene            10345..10725
                     /gene="panD"
                     /locus_tag="ACHJ69_002514"
     CDS             10345..10725
                     /gene="panD"
                     /locus_tag="ACHJ69_002514"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO5015476.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10729..11958)
                     /gene="yadE"
                     /locus_tag="ACHJ69_002515"
     CDS             complement(10729..11958)
                     /gene="yadE"
                     /locus_tag="ACHJ69_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO5015477.1"
                     /translation="MYKQAVILLLMLFTASVSATLPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYVFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRIDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVLYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12022..12462)
                     /gene="yadI"
                     /locus_tag="ACHJ69_002516"
     CDS             complement(12022..12462)
                     /gene="yadI"
                     /locus_tag="ACHJ69_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO5015478.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHETDSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSAFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12567..13337)
                     /gene="yadH"
                     /locus_tag="ACHJ69_002517"
     CDS             complement(12567..13337)
                     /gene="yadH"
                     /locus_tag="ACHJ69_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5015479.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13334..14260)
                     /gene="yadG"
                     /locus_tag="ACHJ69_002518"
     CDS             complement(13334..14260)
                     /gene="yadG"
                     /locus_tag="ACHJ69_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5015480.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFFLDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRT"
     gene            14369..15031
                     /gene="can"
                     /locus_tag="ACHJ69_002519"
     CDS             14369..15031
                     /gene="can"
                     /locus_tag="ACHJ69_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO5015481.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTGTNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15072..15608)
                     /gene="hpt"
                     /locus_tag="ACHJ69_002520"
     CDS             complement(15072..15608)
                     /gene="hpt"
                     /locus_tag="ACHJ69_002520"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO5015482.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15814..18204
                     /gene="gcd"
                     /locus_tag="ACHJ69_002521"
     CDS             15814..18204
                     /gene="gcd"
                     /locus_tag="ACHJ69_002521"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO5015483.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWTGFNDPQEISGTLSADTTPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18400..19950)
                     /gene="cueO"
                     /locus_tag="ACHJ69_002522"
     CDS             complement(18400..19950)
                     /gene="cueO"
                     /locus_tag="ACHJ69_002522"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO5015484.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHSGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20116..20463
                     /gene="yacC"
                     /locus_tag="ACHJ69_002523"
     CDS             20116..20463
                     /gene="yacC"
                     /locus_tag="ACHJ69_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO5015485.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20569..21435
                     /gene="speE"
                     /locus_tag="ACHJ69_002524"
     CDS             20569..21435
                     /gene="speE"
                     /locus_tag="ACHJ69_002524"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO5015486.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTNQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            21451..22245
                     /gene="speD"
                     /locus_tag="ACHJ69_002525"
     CDS             21451..22245
                     /gene="speD"
                     /locus_tag="ACHJ69_002525"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO5015487.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTTEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSEDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITTALWKEMREIYYGRNMPAV"
     gene            complement(22283..22645)
                     /gene="yacL"
                     /locus_tag="ACHJ69_002526"
     CDS             complement(22283..22645)
                     /gene="yacL"
                     /locus_tag="ACHJ69_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO5015488.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22820..25417)
                     /gene="acnB"
                     /locus_tag="ACHJ69_002527"
     CDS             complement(22820..25417)
                     /gene="acnB"
                     /locus_tag="ACHJ69_002527"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO5015489.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFDQLSQYTEKA
                     DGVIFQTAV"
     gene            25772..27529
                     /gene="yacH"
                     /locus_tag="ACHJ69_002528"
     CDS             25772..27529
                     /gene="yacH"
                     /locus_tag="ACHJ69_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO5015490.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEAQSTQ
                     QTAPDISAVTLPATVSVPPVTPAVVKSTFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSGQSWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKIITTTKKVVPVNQPANAPATQSN
                     TVSTSSPVVAEPAPTVIAIEPANPDVVYIPNYNPNVVYGSWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNDWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRDGMPYHDQNMAKRFHQTDVKGGMSA
                     TPLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHNGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAAREKMQTNPQNQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQQRDAARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTARLNNE
                     QRSAVRERLSERGARRLER"
     gene            complement(27771..29195)
                     /gene="lpdA"
                     /locus_tag="ACHJ69_002529"
     CDS             complement(27771..29195)
                     /gene="lpdA"
                     /locus_tag="ACHJ69_002529"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO5015491.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
BASE COUNT         7658 a         7541 c         6954 g         7203 t
ORIGIN      
        1 gatgaatgaa tgtaatactt agtaaaaaac aaacaaacat cattaacaaa gtaacgttca
       61 gttacatggc attgcatcaa aatctcatca tcatgcaatg ctattatctg ctttatggaa
      121 tattactcaa ggatttttat gtctaaaaaa ttaggttttg cgctgtccgg tattatgttg
      181 gcaatggctg ctggtactgc atttgctgct aacgatatgg acggcggtca attaaatatt
      241 agcggtctgg tcgttgataa cacttgtgaa gctcgtgttg atggcggtaa caaagacggt
      301 ctgatcctgc tgcaaaccgc tactgttgct gaaattactg atggtgtact ggataccact
      361 gtaggtgcta aagctaaacc attcagcatt actattgact gcagcaaagc taacccggct
      421 ccgggtacta ctgcaaaaat gaccttcggt tctgttttct ttggtaacag caaaggaaca
      481 ctgaacaacg acatgtctat taacaccccg tctgacggcg taaataccgc tctgcacaat
      541 attgaaggtt ctactattaa acaggttcag gtcaataatc cgggtgatgt ttacagcaaa
      601 actctggatt caacgtctaa atctgcaacc tacgatttta aagcatccta tgttcgtgcg
      661 gatgcaagca aagcagctac tgccggttat gtaaaaacca actcagcata taccattact
      721 taccagtaaa tatcaaccgc ttaaattgat cgggtgaata tatacaggga ataacatcaa
      781 atatttttac ttttgataat tattccctgt tttaattaat tctgtaagga atggtttatg
      841 ttttttaata ctaaacatac cgcagcattg tgcttagcga gctgtatggc ttttagctca
      901 tcagctattg cagacatcgt aatttcaggt acacgcgtag tttataaaag cgaacaaaaa
      961 agcgtcaatg tacgtctgga aaataaaggc aataacccat tgctggttca gagttggtta
     1021 gatactggag atgataacgc tgaaccaggc agtattactg tcccctttac cgctacgccg
     1081 ccagtatcac gtattgatgc taaacgtggc caaacaatca aactgatgta caccgccagc
     1141 agtgctctgc caaaagacag agaaagtgtg ttctggttta acgtactgga agttccacca
     1201 aaacccgatg cagcaaaagc agcaaaccaa agcctgctac aactggcatt tcgcacacgt
     1261 ataaaactgt tctatcgccc ggaaggattg agtggaattt catctgatgc accattggct
     1321 ctgaagtggt cctgggcaac atctgaaggc aaagcagcat tacgcgttga taaccctaca
     1381 ccctactatg tctcttttag cagtggtgat ttagaagcca acggcaaacg ctaccccctg
     1441 gatatgacaa tgattgcgcc gttcagtaac gacgtcatca aggtaaatgg gatgagtggc
     1501 agaacaagct ctgcaaaagt gcattttttc gccattaatg attttggtgg atctattgaa
     1561 ggcaacgcca acctttaatc tggcaaggat ataacacagc aggaagcaga acgtgactat
     1621 taaatctact aatcacctta cccacatcgc cacgttttgc gctctgctgt atagcaatag
     1681 tgctttatgc gctgaactcg tcgaatatga ccatacattt ctgatgggaa aggatgcttc
     1741 aaatatcgac cttagtcggt atacagaagg caacccgaca ctacctagca tttacgatgt
     1801 cagtgtgtat gttaacgatc aacccatcat gagccaaagc attgcttttg ctgttattga
     1861 aggtaaaaag aacgctcagg cctgcattac gcagaaaaat ttattgcaat ttcatatcag
     1921 ttcaccagac aagaatagcg aaaaagccat tttgcttaag cgagacgacg atcttggtga
     1981 ttgcctcaat ctggctgaaa tgatccctca atcatccatt cgttacgatg ttaacgatca
     2041 acgcctggat atcgatgtcc cccaggcatg gattatgaag aattatcaaa actatgttga
     2101 tccctctttg tgggaaaatg gcattaatgc agccatgctt tcctacaacc tcaatggata
     2161 tcacagtgaa agtccagtca gaacaaatga cagtatctat gcggcattca acggtgggat
     2221 aaacttaggc gcgtggcggt tacgtgcctc aggtaactac aactggatga ccaatgtcca
     2281 tagcgattac gattttcaga atcgctattt acaacgcgat cttgcgtcac tacgttcaca
     2341 actggttatt ggtgaatctt acacgaccgg cgagaccttc gattcagtca gcattcgcgg
     2401 tattcgctta tacagtgaca gtcgaatgct gcctccggta ttagccagtt ttgcgccaat
     2461 cattcatggc gtagcgaata ccaatgccaa agtaaccgtt atgcaaaatg gttataagat
     2521 ctatgaaacg acggtgccgc caggggcttt tgccattgac gatcttagcc cgtcaggtta
     2581 tggcagtgac ctcattgtga ctatcgaaga agctgatggc acaaaacgga cgttctccca
     2641 gccattttca tctgttgtgc aaatgctgcg ccctggcgtt ggtcgctggg atattagcgc
     2701 cggtcaggtt ttaaaagaca gtatccagga tgagcctaat ttatttcaag ccagctacta
     2761 ctatggtttg aataactacc tgacaggata taccggtatt cagctaactg ataataacta
     2821 taccgccgga ttgctggggc ttggtatgaa tacccccgtg ggggcttttt ctgtcgatgt
     2881 tactcactca aatgtcagca ttccggacga taaaacctat caaggacaaa gctaccgtat
     2941 ttcctggaac aagttatttg aaaataccag tacatcatta aatatcgcgg cctatcgcta
     3001 ctccacccag cactatctgg gcctcaatga tgcgctgacg ctgattgatg aagtagaaca
     3061 tcctgagcaa gatcttgagc ctaaatccat gcgcaattac tcgcgcatga aaaatcaggt
     3121 cacggtcagt atcaaccaac cgctgaaatt tgagaaaaag gattacggtt cattttatct
     3181 ctcgggaagc tggtcagatt actgggcctc cgggcaaaac agtactaatt actctattgg
     3241 ttatagcaat agtgcgtcct ggggcagtta cagtatcagt gctcaacgtt cgttgaatga
     3301 agatggtcaa accgacgata gcatttatct cagcttcaca attccaatag aaaatttact
     3361 gggtacggaa caccgcagct ccggcttcca gagtattgat acacaattaa acagcgactt
     3421 taagggtaat aaccaactca atatcagcag tagtggttac agcgatacca atcgcatcag
     3481 ctacagcgta aacacgggtt atatgatgaa taaatccagt gatgatttga gttatatcgg
     3541 cggttatgcc agttatgaat cgccgtgggg aacgctgtca ggttcagcct cagcaagcag
     3601 tgataatagt cgccaattct cactcaatac tgatggtgga tttgtgttac atagcggcgg
     3661 gctgacgttc agtaatgaca gttttagtga ttccgacact ctggctgtca ttcaggcgcc
     3721 aggagcaaaa ggcgctcgta ttaactacgg aaacagcacc gtagaccgct ggggttatgg
     3781 tgtgaccagc gccctttcgc cataccatga aaatcgtatc gcactggaca ttaacgatct
     3841 ggaaaacgat gttgaattga aaagcaccag tactgtcgcc gtcccgcgcc agggagccgt
     3901 cgtctttgct gattttgaaa cagttcaggg acaatcggca attatgaaca tcgtgcgaag
     3961 cgatggtaaa aatattccat ttgcagcgga tatttatgat gaacaaaaca atatcatcgg
     4021 caacgtaggt cagggtggtc aggcctttgt tcgcggtatc gggcaggaag gaaatatacg
     4081 aattacctgg attgaagagg gcaaaccggt cagttgtttt gctcattatc aacaaaacac
     4141 gacatcagaa aaaatagcac aatctattat tctgaacgga cttcgttgtc agatacagta
     4201 accacaaggg acttccaatg ataaaaatca caccacataa aataacaata ctcatgggac
     4261 tattgttatc gccttcggta tttgcaacgg atgttaatgt cgattttacc gctaccgtga
     4321 aagcaacaac ctgtaatatt acacttaccg gaactaatgt cactgataat ggaaaagata
     4381 agtacacctt agttattccc tcgatgggaa tggataaaat agccaataaa acagcacaat
     4441 ctgaagccaa ctttaaattg gtagccaatg gatgcagcag tggaattagc tggattgata
     4501 caaccctgac aggaaatcaa tcaggaagtt cacccgcgct aattatcccc ctggccagtg
     4561 atacaacctc cactacaagc tatatcggaa tgggatttaa aaggaaggcg acttcaggcg
     4621 atacatttct gaaacctaat agcgcagaat atatacgttg gagtgcatca gaaattagca
     4681 ccgatggact tgagatgact gtcgcactgc gtgaaacaag tgtagggaaa ggtgttccag
     4741 gcaagtttcg cgcactggca acttttaact tcagctatca ataacaagga tgacaatgat
     4801 gatattaaaa aaattatgtt atggattatc gggtagtcta tttctggctt catcactgtt
     4861 aaccatttcc tcacatgcgg ccactgacag tattggcctg actgttacta ctgacgtaga
     4921 aatgggtacc tgtacatcaa cgttgactga tgacggtaca actaaaattt ccaccatcaa
     4981 tttcggtgat gtatatattt ctgaaattaa tgccgaaact aaaattaaaa ccttcaaatt
     5041 acagttcaaa gactgtgcgg ggattcccgg gaaaaaggcc aaaataaaat tgactacacg
     5101 agcattatgt gaaggtaatt ctaataatgg tcctggattt gctaacgcat caacggcaac
     5161 agcaaaagca gcggcggtcg ccgttgaggt ctggagcaca tctacgccag gtaagaacgg
     5221 cgctaaacaa ttcagttgtg tcacccccgc aacagaagaa gtcagtattg ctaatgcaac
     5281 gggcagcaat gttgtcgatt acccaatgag tgcggtgttg gtggtcgcaa aagataaaac
     5341 tgtcacaaat gtcactgcgg gtgatttcac tgccccggca acattcaccg tgacttataa
     5401 ctaacaggca gactgattca ggaactaaat atgcgtccca ctcaacgtaa tctgatgaaa
     5461 aaatcaattc tgattctgtc gttgctattc agcgccactt cccctgtggt tgcaggacag
     5521 gatgttgaca ttgttgcaaa tataaaaaat aacacctgcc aaagtggaat cagcaacaac
     5581 ggaaatattg atttaggtgt cgttggcgtg ggcgatttta ccggaaacat ttcagccgaa
     5641 aattatcacc ccggtggaaa agagttcact attacagtac aggactgcac acttcagggt
     5701 acagggaatg tgttaaatca gttacatatt aattttcgcg cacttagtgg cgtaatggcg
     5761 acaggttcca gtcaaatatt cgctaatgaa gacaactccg gggctaaaaa tgttggggta
     5821 gttatatttt ctattcagga cccggcaaat accttcaacg ttcttagtgc cactggtagc
     5881 tcacgttccg tttatccggt catgtcatca gcactaaata attcatcctg gaaatttagt
     5941 acccgaatgc aaaaaatcga tccagcactg aatgtcacct ctggcccact gataagccat
     6001 gtgttagtag atatctacta cgaataacag acaaagtgct acaagtatta tcttgcagca
     6061 tgacaatgga gtaaaaatga agacattctt cagatacttt ctttttttag cactatgctc
     6121 ttgttgttat acagcaagcg ccggcaccga tgatgacgtc ggctatattg ttgggaataa
     6181 ttacggtgtt gggcctggag accaaaaatg gcgcgaaaca ggccccaatg gagatgttac
     6241 cgtcaaattc cggtatggtt caggcaccaa taatttagtt ttttataaac caacccaact
     6301 tggcccaacc ggggtgtcat taaaatgggc gcagttagat tccgcttcgg gtggaggttt
     6361 cctttactgt aacaggagtg ataactcaag cggcgcacca atgtctattg aacataaaat
     6421 ggttgattcc ggaaaaagtt atggcggaca taaactcttt aagacatctg ttccaggcct
     6481 gtattacacg ttagcaatat caaacatttg gtcaacatat acatatactg atatcaaccc
     6541 atcaggcatg tatattggcg attctacgag ccaatctttt aactggcgtg gcgaaagcga
     6601 gcaaacacta tattggtctt gcaacaacgc gaatagcagc aaaaaatact gggccgttgg
     6661 tggcgttatg caaacgctca cgattgaatt ttataccgat accgacttta acccgacaac
     6721 gaatcaacgg gtaacattat ccagaacaga tagttatctc tactcattca aagcatataa
     6781 tgctggcatt agtatcaaga gttattttct taaaatcgac tttgacctga cagatatcgt
     6841 attaacaaac cctacctgtt ttactgctgc gcttagtggc ccatcggtaa atggttcaac
     6901 ggttaaaatg ggtgattata gccctgcaca aataaaaaat agcgcaacag cggttccttt
     6961 tgatatcacg ctacagaact gtattcgtgt tcgcaatata gaaacaaaac tcaagtcaaa
     7021 caaggtgggt agtgtaagca aagaacttct ggctaacaca ctcacaggaa atgacgcagc
     7081 aaaaggcgtt ggtgtactga ttgaaggttt aaaaaacact aaaagtgcac aaatggtact
     7141 gaaaccaaat aatgcaacgt ctatttataa agattacgaa acagaagatg acactaccgg
     7201 aggaatttac ccggataaag gaaacggtac ctcacaacct cttcatttcc aggctacatt
     7261 aaaacaagac ggtaatattg ctatagaacc gggggatttt aaagccacca gcacttttca
     7321 ggttacctac ccctgataac actatcgcac cgccagacaa cggcggtgcg atacggataa
     7381 atcattgccc ccaccaatgt gactgataaa atgcgagaca gcacaacatc atttatcagg
     7441 atacgctatg aaaccaacca cgattgcttc attgcagaaa tgcaagcagg acaaaaaaca
     7501 tttcgcaacc atcaccgctt acgactatag cttcgcaaaa ctgtttgccg atgaagggat
     7561 taacgtcatg ctggtaggcg attcactggg catgacggtc caggggcatg actccacgct
     7621 accggttacc gttgccgata tcgcctatca caccgccgct gtgcgtcgcg gcgcgccaaa
     7681 ctgccttctg ttagcagacc tgccatttat ggcgtatgcc acgccggaac aagcctttga
     7741 aaacgccgca acggtaatgc gtgccggagc caacatggtc aaaattgaag gcggtgagtg
     7801 gctggtggaa accgtacaaa tgctgaccga acgtgccgtt ccagtttgtg gtcatttagg
     7861 cctaacccca cagtcagtga atattttcgg tggctacaaa gtccaggggc gcggcgatga
     7921 agcgggcgat cgactgctca gcgatgcatt agccttagaa gctgctgggg cacaactgct
     7981 ggtactggag tgcgtgccgg ttgagctggc aaaacgcatt accgaagcac tggcaatccc
     8041 ggttatcggt attggcgcag gcaacgtcac tgacgggcaa attctcgtga tgcacgacgc
     8101 gttcggcatt accggcggtc atattcctaa attcgccaaa aatttcctcg ccgaaacggg
     8161 cgacatccgc gcggctgtgc ggcagtatat ggctgaagtg gagtccggcg tttacccggg
     8221 cgaagaacac agtttccatt aaggagtgac gttgtgttaa ttatcgaaac cctgccgctg
     8281 ctgcgtcagc aaattcgccg cctgcgtatg gaaggtaagc gcgtggcgct ggtgcccacc
     8341 atgggtaacc tgcacgatgg ccatatgaag ctggtcgacg aagccaaagc ccgcgccgat
     8401 gtggtcgtcg tcagtatttt cgttaaccca atgcagtttg accgcccgga agatctggca
     8461 cgttatccac ggaccttgca agaggactgc gaaaagctga acaaacgtaa agtggatata
     8521 gttttcgcgc cttcggtaaa agagatctac ccgaacggta ctgaaaccca cacctatgtt
     8581 gatgttcccg gtctttccac catgctggaa ggtgccagcc gtccgggaca ttttcgcggc
     8641 gtttcgacta ttgtcagcaa gctgttcaac ctggtccagc cagatatcgc ctgcttcggt
     8701 gaaaaggatt ttcagcaact ggcactgatc cgcaaaatgg ttgccgatat gggcttcgat
     8761 attgagattg tcggagtgcc gattatgcgc gcgaaagacg gtctggcgct cagttcccgt
     8821 aacggctatc tgacggcgga acaacgtaaa attgcgccgg gtctctacaa agtcttaagt
     8881 tcaattgccg acaaattgca ggctggcgaa cgggatctcg atgaaattat tgctattgcc
     8941 gggcaagaac tgaatgaaaa aggtttccgt tccgatgata ttcagattcg cgatgccgac
     9001 accttgctgg aaatctctga aaacagcaaa cgagcggtga ttctggtagc cgcctggctt
     9061 ggcgatgctc gcctgatcga caacaaactg gtcgaactgg cgtaatgctt aactggcgct
     9121 acggctgatg gcgccagtta ttcattgcca cgttatctgt aggcaattat tcataatgca
     9181 gacaacttag ttaatcattt tatcaatctc agcaagaggt aaatttacca tctctgcaac
     9241 gtcttcccga gacattcctt tactcagaag acgcagggcg aattcctgac ttacttcctg
     9301 tcttccctgc tgaatcccct tctcttcaaa ccactgcgcc agcgtcatca tagactcccc
     9361 tcccgtttcc ctgtctctca ccacaccata aaacaaatcc gcttgttcag tatgaccgcg
     9421 ttgcagcata tagttttgca tggcaactaa ctgacttccg ctagtgtacc cttcgtcaat
     9481 cagcgtgacc agttgctcca gcaataacat taagtcgcgc tggcgaatat gtttttgcag
     9541 cagttcgaga atcgcaatcc gccgatgttg catgatttcg tcatccggtg tgatagtgat
     9601 atccaccagc gggaaaggac tgttatagac gcgtcgcgcc agttccggtg agtaaaacat
     9661 atcaaaccag cacattgata gcggataagg tgtggcctcg ccctgataaa acagaattgg
     9721 caccaccagc ggcagcttat cgtggtcagc ctccagatgc cggtgcatgg cagcaataga
     9781 ataacgcatc atgcgaaagg ccattttctt atccggcttg ctttgatgtt caatcacaac
     9841 atgcaaataa ccggggttgc cctgcatttg cacggaataa agcacgtcag tgctgtgccc
     9901 tttcaggctt tcttcaataa aactccccga ctcaagatgc agcgtgtcga ggtcacacag
     9961 ttcgcgtagc tccgctggca aatggatatc cagaaagtcg cgagccgttt ccgcatgcat
    10021 taaaaattgt ttaaacaccg catcatgcgg tgtggtactc ggtgcatcca tcttccctgc
    10081 cttccgtgtg gtcttgcgtg aaggcagtat ccagtcaagg tgtgccgtcg tcagccgcct
    10141 ctttagtgtt taccgagcag tgttcagata aatttcaatt ggttacaggc gttacatggc
    10201 acgcttcggc acggtacgca aactgctgct gcaaatgccg tcagatgagt agacactaaa
    10261 caaaaatcgg gcaatactgc gtgagaattt cctcaaagca ggcatcgcct gcttcgttaa
    10321 cgacagggta gaaaggtaga agttatgatt cgcacgatgc tgcagggcaa actccaccgc
    10381 gtgaaagtga ctcatgcgga cctgcactat gaaggttctt gcgccattga tcaggatttt
    10441 cttgacgcag ccggtattct cgaaaacgaa gccattgata tctggaatgt caccaacggc
    10501 aagcgtttct ccacctatgc catcgcggca gaacgcggtt cgagaattat ttctgttaac
    10561 ggtgcggcgg cccactgcgc cagcgtcggc gatattgtca tcatcgccag cttcgttacc
    10621 atgccagatg aagaagctcg cacctggcga cccaacgtcg cctattttga aggcgacaac
    10681 gaaatgaaac gtaccgcgaa agcgattccg gttcaggttg cctgattgtt atccctgcgg
    10741 ctggttactc accagccgcg acatcgtctc cagcgaatcc gttcttaaga tataaagtcg
    10801 ttttagtaac aacggattat cccccggttt tactttgcct ttcatggtcg tcaccgcgag
    10861 gtgaaatccg gcttcttttg ctgccttcac ggctttgtca ttaaatccgc caaatggata
    10921 agaaagatac aaaacatgtg gattaaattg cgccaaagcg cggcgtgaac gtgcaaaatc
    10981 aaacagaata ttgtgctcac tacggctcag caatataggc cgccgataac catctatccg
    11041 atgcaaaaaa tgagtatgtg actggaaatc aaatacatcg cgaatttcgt taagttcaga
    11101 aacgctcata aattgcagcg attttgggtt ccatttctgc gggtgacgtt tgatgcgcga
    11161 ggtaacaata aacgccgtcg ccttcatacc atattgtttc aacacaggat acgcatagcg
    11221 gctcaccgac ttgaggccat catcaaaggt aattaccacc gctcgcgcag ggagattgat
    11281 cttattcttc acgtaacctt ccaactgcgc catgctcagt gtcgcgtatc ccctgtcgcg
    11341 cagccaggcc atctggttat tgaaagcgcg taccgaggtg gtcgtcgaag tatggcgaaa
    11401 acgggtgttt tcttcgtcgc gcaaaatatg gtgataggtt agcaccggca ggccattatc
    11461 gggttgggca tccagcgcgc tgatataggc caatcgatcg ccaatacgga tctgatacca
    11521 ggtttgattt aacctgtctt tcagtttatg caaaatcggg tagcgcaaat tgtccgccag
    11581 tactccaaat ggcgcactgc ccacactcgg cgcgttataa accggcgtat ctttccaggt
    11641 tattaagttc tgattactca gcggcttgtt gaggtcaccc aaaccatctt caactttttg
    11701 tcgcccctga accggctcga gatggccttt atcgataaac cctttaccaa agccaaaatt
    11761 aaatacgtaa taacttgcgg cagtgggctc cacagcaatg atttgcccgg cccgaatatt
    11821 ccctaccgtc accattttgt cgccaatttg cgcccagacc gcggcatttt cgatggtttg
    11881 catataacgg gcaggtaatg tggcactgac gcttgcggta aacagcatca gcaggagaat
    11941 aacagcttgt ttgtacatga aaaaataacc gagggtgaaa caacgctttt attgtagcaa
    12001 aagcgtcatc agaatcgcgg gttaatactc atacaaatta tgtttgagga cgaaaggcgg
    12061 atttttttgt tgttggtgcc aaagactact cacctccggc gcacccagtt cgacaatacg
    12121 ctcgcgaaac gctgaactgg tcatggtttc acggcaagcc atcatctgtt caattaacgg
    12181 taacgtgaca ccagaaatca cttcgcaacg agagtgtttg tggcttaata atgaagccac
    12241 gcggtacggt ggcgctccgg ctatatcggt taaaaagata acgccctcac cagagtccgt
    12301 ttcatgcaag gcatcgcaca tcatgcggct gagcatatta gagcttaatc cgcgccagaa
    12361 attcacggcc cggcactgaa gaagtgcccc atgttttttc tccagcgcat ccagtatctc
    12421 ttgcgcccgg tcatcgtgac aggtaattac ccaacctaac atcgccttcc tccttagccg
    12481 aaaatcagtc taccctgacc attgttgata aagggtgata gccgtcaaaa ctcctctccc
    12541 ccaaatccag aggagaggaa atagccttag ctacgcaaac cacgtccacg ttggatcagc
    12601 gaccaacaga tcaaataaaa cgccacaata aagaccacca gtacgccaaa ggtagtgacc
    12661 agcggaacat cattgatacc gaggaagccg tagcggaaac cactgatcat ataaacgatt
    12721 gggttcaggt gcgacagccc ttgccagaac ggcggcaaca aagtcaggga gtaaaacacc
    12781 ccgcccaaat acgtgagtgg cgttaacaca aaggttggca ccaggctgat gtcatcgaac
    12841 gttttagcaa acacgccgtt cagcaaaccc gcaagggaga atagcaccgc agtgagcacc
    12901 agggttaagg caacgaatac ccacgaatgc acctgaaacg gcacaaaaaa cagtgaaatt
    12961 gccgtcacca gaataccaac aaacagacca cgcgccacgc caccaccgac atatccggca
    13021 ataatgacat gagtcggaac cggcgctacc agcagctctt caatattacg ctggaacttg
    13081 gcaccaaaaa atgatgacgc gacgttagcg taggcgttgg tgatcaccga catcatgatc
    13141 agccccggta cgatgaactg catgtagctg aagccatgca tatcgccaat acgcgaacca
    13201 atcaggttac cgaagataat aaagtaaagg gtcatggtga tgactggcgg caccagcgtc
    13261 tgcacccaga tacgcataaa gcgatggatc tctttcgccc agatgctttt tagcgccacc
    13321 cagtaaagat gcatcatgtg cgatctcctt gtttttcatt aaccagtgaa acaaacagct
    13381 cttccagacg gttagctttg ttacgcatac ttaatacctg aatgccctgc tcacttaact
    13441 gcgtaaatac gctgttgatg ccctgctcac gcagcacttc aacttccagc gtggcggtat
    13501 ctaccagtcg atactgatag ccatcgagct tcggtaacgg acttttcggt gcgagatcga
    13561 gaaaaaaggt ttctgatttc agcttcgcca gcagcgcctt catcgaagta ttttccacca
    13621 gctcaccgtg ttgaataatg ccgatattgc ggcacagcat ttctgcttct tccaggtaat
    13681 gtgtggtgag aatgatggtg gtgcctttgt cgtttaaatc cttcaaaaag ccccacattg
    13741 agcggcgaag ttcaatatcc acgcctgcgg tcggttcgtc gagaatcagt agtttaggtt
    13801 catgcattaa cgcacgggca atcattaaac ggcgcttcat cccgccagat aacatacgcg
    13861 cacgttcgtt gcgttttccc catagatcga gttgtttaag atacttttcg ctgcgaatgt
    13921 acgcttcttt gcgctccaca ccgtagtacc ctgcctgatt caccacaatt tgctgcacgg
    13981 tttcaaacgg gttgaagtta aattcctgtg gcaccagacc caactgacgt ttagcgttta
    14041 cgacatcttt ctcgagatcg taaccaaata cgctgacccg cccggaggtt ttatttacca
    14101 gagagctgat aataccgata gtggtcgatt ttccggcccc gttcggcccg agaagcgcat
    14161 aaaaatcacc cgcttcgacc tgcaaatcga ttccacgaag cgcctgaacg ccgcctggat
    14221 aggttttttt aagctgttga agttccagtg caatggtcat aaatttttac ttaccttacg
    14281 ttcttacact ttatctgtgg tttaaatcgt cccggagttg ccctatatta gccaaacgta
    14341 attatttggt tacaggtcgt taacctccat gaaagacata gatacactca tcagcaacaa
    14401 tgcactatgg tcaaaaatgc tggtggaaga ggatcccggg ttttttgaga aactggcaca
    14461 agcgcaaaaa ccgcgctttc tatggattgg atgttccgac agtcgcgttc ctgcagaacg
    14521 tttaaccggt cttgagccgg gcgaactctt tgttcaccgt aatgttgcta acctggtcat
    14581 tcacaccgac ctgaactgcc tttccgtggt tcagtatgca gtggatgtac tcgaagttga
    14641 acacattatt atctgtggcc actacggttg cggcggcgta caagccgcag ttgaaaaccc
    14701 agaactgggg cttatcaaca actggctgct gcatatccgc gatatctggt tcaaacatag
    14761 ctcattgctc ggcgaaatgc cgcaagagcg ccgtctggat accttgtgtg aactgaacgt
    14821 catggaacag gtgtataacc tgggccactc cactattatg caatcagcgt ggaaacgcgg
    14881 gcagaaagtc accatacacg gctgggccta cggcattcac gacggcttgc tgcgtgatct
    14941 ggatgtcaca gggactaacc gcgaaaccct tgagcaacgt taccgtcacg ggatctccaa
    15001 cctcaagctg aaacacgcca accacaaata aaaatgccat gccggatgca acacatccgg
    15061 caacttcaca cttactcgtc cagcagaatc actttgccga tatacggcag atgacggtaa
    15121 cgctgtgcgt aatcaatgcc gtaacccacc acaaactcat ccgggatcga gaaaccgata
    15181 aattctaccg ggacgttcac ttcacgacgg gacggtttat ccagcagcgt acaaatcgcc
    15241 aacgacttcg gttcgcgcag gcttaagatc tcacgcactt tcgacagtgt attccccgag
    15301 tcgatgatat cttcaacaat cagcacgtcc ttgccacgga tatcttcatc caggtctttg
    15361 aggattttca catcacgggt ggtggacatg ccgctaccgt agctggaggc ggtcataaag
    15421 tcgacttcat gagatacctg aacttcacgg cacaggtccg ccataaacat aaatgagcct
    15481 cgcagcagac ccaccagcac catatcgctg ccgctgtctt tgtaacgctc agtaatctga
    15541 cgacccagtt cggcgatacg cgctttaatc tccgcttcgg ggatcattac ttctacagta
    15601 tgtttcatat ctataaccat atgatttaaa aacaaatcac ttcgcacggg caggttttgg
    15661 caccgcaaca aagcgcaaga cacgcagtat accagtaaaa ctattcattc gcggaatctg
    15721 ttaatacagc tcgaattgtg atgacgatca cacatgttaa aacctataat taattgctac
    15781 taaaatatta atgaattgaa atggtgtctc tttatggcaa ttaacaatac cggctcgcgg
    15841 cgattactcg tcacgctaac agcccttttt gcagcgcttt gcgggctgta tctactcatt
    15901 ggcggaggct ggctggtcgc gattggcggc tcctggtact accctatcgc tggccttgtg
    15961 atgctcggcg tcgcctggat gctgtggcgc agtaaacgcg ccgcgctttg gctgtacgca
    16021 gccctgctgc tcggcactat gatttggggc gtctgggaag ttggtttcga cttctgggcg
    16081 ctgactccgc gcagcgacat tctggtcttc ttcggcatct ggctgatcct gccgtttgtc
    16141 tggcgtcgcc tggtcattcc tgccagcggc gcagttgccg cactggtggt cgcactgctg
    16201 attagcggtg gtatcctgac ctggaccgga tttaacgatc cgcaggaaat cagcggcacc
    16261 ttaagcgccg ataccacgcc agcagaagcc atctcccccg ttgccgatca ggactggcct
    16321 gcctatggtc gcaatcagga aggtcaacgc ttttcaccac tgaagcaaat taacgctgat
    16381 aacgtccaca agctgaaaga agcctgggtg ttccgtactg gcgatgtgaa gcagccgaac
    16441 gatccgggcg aaatcaccaa tgaagtgacg ccaattaaag tgggcgacac cctttacctg
    16501 tgtaccgcgc atcaacgtct gtttgcgctg gatgccgcca gcggtaaaga gaaatggcat
    16561 tacgatcctg agctgaaaac caacgagtcc ttccagcatg taacctgccg tggcgtctct
    16621 tatcatgaag ccaaagcaga aactgcttcg ccggaagtga tggcggattg cccgcgtcgt
    16681 atcattcttc cggtcaatga tggtcgtctg attgcaatta acgctgaaaa cggcaagctg
    16741 tgcgaaacct tcgccaataa aggcgtgctc aatctgcaaa gcaatatgcc ggacaccaaa
    16801 ccgggtctgt atgagccgac ttcgccgccg attatcaccg ataaaaccat cgtgatggcc
    16861 ggttcagtca ccgacaactt ctcaacccgc gaaacgtctg gcgtgatccg tggttttgat
    16921 gtcaacaccg gggagctgct gtgggctttt gatccgggcg cgaaagatcc gaatgcgatc
    16981 ccgtctgacg aacacacctt tacctttaac tcaccaaact cgtgggcacc agcggcctat
    17041 gacgcgaagc tggatctggt ctatctgccg atgggcgtga ccacgccaga tatctggggc
    17101 ggtaatcgca caccggaaca ggaacgttat gccagctcga ttctggcgct gaatgccact
    17161 accgggaaac tggcgtggag ctaccagacc gttcaccacg acctgtggga tatggatctt
    17221 ccggcacagc cgacgctggc ggacatcacc gttaatggtc agaaagtgcc ggtcatttat
    17281 gctccggcga aaaccggcaa catttttgtg ctcgatcgtc gcaatggtga actggtggtt
    17341 cctgccccgg aaaaaccggt tccccaaggt gctgctaaag gcgattacgt gacccctact
    17401 caacccttct cggaactgag cttccgtccg acgaaagatt tgagcggtgc ggatatgtgg
    17461 ggagccacca tgtttgacca gttggtgtgc cgcgtgatgt tccaccagat gcgctatgaa
    17521 ggcattttca cgccgccatc tgaacagggt acgctggtct tcccgggtaa cctggggatg
    17581 ttcgaatggg gcgggatttc cgttgatccg aatcgtgaag tggcgattgc caacccaatg
    17641 gcactgccgt ttgtttcgaa actgatccca cgcggtcctg gtaacccgat ggaacagccg
    17701 aaagatgcca aaggcacggg tacggaatcc ggcatccagc cgcagtacgg tgtaccgtat
    17761 ggcgtcacgc tcaacccgtt cctctcaccg tttggtctgc catgtaaaca gccagcatgg
    17821 ggctatatct cagcattgga tctgaaaacc aatgaagtgg tgtggaagaa acgtattggt
    17881 acgccgcagg acagtatgcc attcccaatg cctgtaccgg tgccgttcaa tatgggtatg
    17941 ccgatgcttg gcgggccaat ctccacggcg ggtaacgtgc tgtttatcgc cgctacggca
    18001 gataactacc tgcgcgctta caacatgagc aacggtgaaa aactgtggca gggtcgttta
    18061 ccagcgggtg gtcaggctac gccaatgacc tatgaagtga atggcaagca gtatgtggtg
    18121 atctccgcag gcggtcacgg ttcatttggt acgaagatgg gcgactatat tgtggcttat
    18181 gcgctgccgg atgatgtgaa gtaaggcttg ctcagattgc tgacaacgtg cgatttgttc
    18241 atgccggatg cggcgtgaac gccttatccg gcctacaaaa catttgcaaa ttcaatacat
    18301 tgcgcgtagg cctgataagc gtagcgcatc aggcagtttt gcgtttgtca tcagtttcat
    18361 gcccggagag atccgggcat atttccgaat acggtctttt tataccgtaa accctaacat
    18421 catccccgta tcttcatgct ccagcagatg gcagtgcgcc atataagcat gttctttcgg
    18481 tgcatcgtga ttaaacttca ccagcacttc gctgacatta ccttctacct taacggtatc
    18541 tttccagcct gcgcgatgcg tcgctggcgg tttgccattt tctgacaaga tacggaactg
    18601 cgtaccgtgg atatggaacg gatgcagcat catgtcgccc acgccagaga taacccaacg
    18661 ttcgtactgc cctttcgccg ccgcaaacat cggcttattc atatcaaacg cctgaccgtt
    18721 gattttgttg gcatggtgga aatcgaactt cccgctgtgg ttcatatgat tcatattgcc
    18781 gtgccccata tggcccatca tctggctgtg atccatcccg gccatcgcct gatcgccata
    18841 tttctccatc agcatctgca tccccatcat atcgagcatt gggtccatag aaagttgcag
    18901 cttgcgtacc gtcagccctt ccagcgaagg taacgcaggc aggctactta atgtgtctgg
    18961 caaagcacca gaggcactaa tggcaatcgg ctgaatccgc attaccggat gcggcttgtc
    19021 aaacggcgca atcgccatcc ccatctggct gaccggcagc gtcaccaggt caaagggttt
    19081 gttgtcgtta acctccacca gcacttcaaa acgctcgccc atcagcactg gcagctcgtt
    19141 caccttcacc ggttcaggta gcagaccgcc gtcgctggca atcacataaa gcgggcgatt
    19201 gtcgctggtg gcgaaattga gcgagcgggc attacagcca ttgagcaaac gcaggcgcag
    19261 ccagccacgc ggggcagcgt gttgcgggta gattgcaccg ttggtcagca acgtatcgcc
    19321 aaaccaaccc acagcggcgg tcatcacatc cagttgataa tcaatctgcc cgtcagcgtt
    19381 aaatttttta tcctgaacaa tcaccgggac atcatcaata ccccactgtt ttggcagcat
    19441 taatttcagg atctcgtcat cttcaatcac caccaaacca gccagcccca tcgccacctg
    19501 acgtccggtc ttgccatgtt gatgtggatg gaaccagcag gtagcggcag gttgatcaac
    19561 gttcaacgtc accgagcgct tgccacctgg cggaataatt ccctgcgggc cgccgtccac
    19621 ttcacccggt acttccagcc cgtgccagtg caacgtcgtc tcttccgtca gttggttgta
    19681 gatatcaacc gttaccgctt tgccacgctg taatttcacc gccggcccca gcagattgcc
    19741 gttatagccc caggtagttg cggttttccc gccaaaggtg gactgacctg cgccaatagt
    19801 taactgaatg cgattacggg catcggtcgt gagcaaatca gggattggta acgttgggcg
    19861 ttccgccgca aatactgcgc ggctccacag cggcaaggct gaagccacac ccagcgcgac
    19921 ggaatatttc aagaaatcac gacgttgcat agttatttcc ttattcttaa gcaggcgaaa
    19981 caaaatcaaa cgttgagcat agtccttccc cttacgggaa ggtcaagccg cagacacaat
    20041 ttaatcgaca ataatggtcg tcgcctcgct cgcagtgtgc taacgtttat cttctttaag
    20101 cccctggtag aagcaatgaa gacgtttttc agaacagtgt tattcggcag cctgatggcc
    20161 gtttgcgcaa acagttacgc gctcagcgag tctgaagccg aagatatggc cgatttaacg
    20221 gcagtttttg tttttctgaa gaacgattgt ggttaccaga acttacctaa cgggcaaatt
    20281 cgtcgcgcgc tggtcttttt cgcccagcaa aaccagtggg atctcagtaa ttacgacacc
    20341 ttcgacatga aagccctcgg tgaagacagc taccgcgatc tcagtggcat tggcattccc
    20401 gtcgctaaaa agtgcaaagc cctggcccgc gattccttaa gcctgcttgc ctacgtcaaa
    20461 taatccctga tacctttttg tagaaatatt gaccgtgcat ccgcggtcaa tgttagctat
    20521 tatgttgcgc ccttttttta cgggtgttaa caaaggaggt atcaacccat ggccgaaaaa
    20581 aaacagtggc atgaaacgct acacgaccag tttgggcagt actttgcggt agataacgtt
    20641 ctgtatcatg aaaagaccga tcaccaggat ctgatcattt ttgagaacgc cgcatttggt
    20701 cgcgtaatgg cgctggatgg cgtagtacaa actaccgagc gcgacgagtt tatctatcat
    20761 gagatgatga cccatgttcc gctactggcc cacggtcacg cgaaacatgt gctgattatc
    20821 ggcggcggcg acggtgccat gctgcgtgaa gtaacccgac ataaaaacgt tgagtcaatc
    20881 acgatggtgg aaatcgatgc gggtgtcgtg tcgttctgcc gtcagtatct tcccaaccat
    20941 aacgccggta gttacgacga tccgcgcttt aaactggtga tcgacgatgg tgtcaatttc
    21001 gttaatcaaa ccaaccagac ctttgatgtc attatctccg actgcaccga tcctatcggt
    21061 cccggcgaaa gtctgtttac ttctgcattt tatgaaggct gcaaacgttg cctgaatcct
    21121 ggcggtatct tcgtcgcaca aaacggcgtc tgctttttac agcaggaaga ggccatcgac
    21181 agtcatcgca aactcagcca ttacttcagc gatgttggct tttatcaggc ggcgatcccg
    21241 acctattacg gcggcatcat gacctttgca tgggcgacag ataacgacgc cttacgccat
    21301 ctctcaaccg aaattattca ggcgcgtttt ctcgcttctg gcctgaaatg ccgttattac
    21361 aatccggcag tccatacggc agcttttgcc ttaccccagt atctgcaaga tgcactggct
    21421 tcacagccgt cctaaggaga agataagaaa ttgaaaaaac tgaaactgca tggctttaat
    21481 aatctgacca aaagtctgag tttttgtatt tacgatatct gctacgccaa aactaccgaa
    21541 gagcgcgacg gttatattgc ttatatcgat gaactctata atgccaaccg tctgaccgaa
    21601 atcctgtcag aaacctgttc cattatcggg gccaatattc ttaacatcgc ccgccaggat
    21661 tacgagccac aaggtgccag cgtcactatt ctggtgagtg aagaaccggt tgacccgaaa
    21721 ctcatcgaca aaacagaaca ccccggccca ctgccagaaa cggtcgttgc ccatctcgat
    21781 aaaagtcata tttgcgtaca tacctacccg gaaagtcatc ctgaaggcgg tttatgtacc
    21841 ttccgcgccg atattgaagt ctctacctgc ggcgtgattt ctccgctgaa ggcgctgaat
    21901 tacctgatcc accagcttga gtccgatatt gtgactattg attatcgcgt gcgcggtttt
    21961 acccgcgaca ttaacggtat gaagcacttt atcgaccatg agattaattc gattcagaac
    22021 tttatgtccg aagatatgaa ggcgctgtat gacatggtgg atgtaaacgt ctatcaggag
    22081 aatatcttcc ataccaagat gttgcttaaa gagttcgacc ttaagcacta catgttccac
    22141 accaaaccgg aagatttaac cgacagcgag cgccaggaaa ttaccactgc gctgtggaaa
    22201 gaaatgcgcg agatttatta cgggcgcaat atgcccgctg tttaacgtct caggcggagc
    22261 acggaggctc cgcctgattt acttacttct gctgcacgaa attacggtaa gccgccacca
    22321 cctgaagaaa atcctcaacg ccgcacagcg acaagctctc ttcatcgtag tagttcatcc
    22381 cctcttccat ttcatcgcca gcgaattcca gttgattggc gcgaaccatc acctcttcac
    22441 catccatcca tagcgtgtat tcatgccctg cccgttgcca ggaacgttcg ctacctttca
    22501 atgcgtgcgc cgcttgttct acttcatcaa gcaaggccag gttttctttc acctcttcat
    22561 taaaccagtg cccgaccact tcatgcccca tggacatgcg cacctttacc actccggtaa
    22621 tatcgcgcag aaattcgtaa tccatagtgt gttcctctgc tcctggcaat gcgccgaaat
    22681 cgcattgcgc cattgatgta attatcgcag ccttaccgga gaaaaacagc ggaacggcga
    22741 tggtttagaa ataaaaaatg cccgggaatc tcattcaccg ggcattgtgt cgtttatgcg
    22801 cagcgcgtgc gcttacttat taaaccgcag tctggaaaat caccccgtcc gctttttcgg
    22861 tgtactgaga aagctggtcg aagttcagat aacggtaagt atcaacggct gttttatcta
    22921 cctgcgccac gtaggtctgg tactcttccg gcgtcggcag tttgccaatc agcgccgcaa
    22981 cagcagccag ttccgcagaa gccaggaaga cattcgcgcc agtacccaaa cggttcggga
    23041 agttacgggt agaggtggaa accaccgtcg caccgtctgc tacacgcgcc tggttaccca
    23101 tacacaggga acagccaggg atctcgatac gagcaccact cttaccgaag acgctgtagt
    23161 agccttcttc ggtcagctgc gcagcgtcca tacgcgttgg cggtgccacc cacaggcggg
    23221 tcggcaactg acctttgtgc gcatccagca gtttaccagc agcacggaag tgaccgatgt
    23281 tagtcatgca agaaccgata aatacttcgt cgatcttctc gccctgtacc gcagacagcg
    23341 gacgtgcgtc gtccgggtca ttcggtgcac agaggattgg ctctttgata tccgccagat
    23401 cgatgtcgat cactgccgcg tattccgcat ctgcatcggc ttccagcagc tcaggattcg
    23461 ccagccattt ttccatgccc tgaatacgac gctccagggt acgacgatcg ccgtaacctt
    23521 ccgcgatcat ccacttcagc aggacgatgt tagagttcag gtattcaatg atcggttctt
    23581 tgttcagctt gatggtacaa ccggcagcag aacgctcggc agacgcatcg gtcagctcaa
    23641 atgcctgctc aactttcaga tccggcagac cttcaatttc cagaatgcgg ccagagaaga
    23701 tgtttttctt acctttcttc tcaacggtca gcagaccttg tttgattgca tacagcggaa
    23761 tggcatgtac cagatcgcgc agggtgatac ccggctgcat tttgcctttg aagcgtacca
    23821 gaacggattc tggcatatcc agcggcatta cgcctgttgc ggctgcaaat gccaccagac
    23881 cagaacccgc cgggaaagag ataccgatcg ggaaacgggt atgggagtca ccaccggtac
    23941 cgacggtatc cggcagcagc atacggttca gccaggagtg gattacgccg tcacccggac
    24001 gcagcgacac accgccacgg ttcataatga agtccggcag cgtgtggtgc gtgttcacat
    24061 caactggctt cggatacgcc gcggtgtggc agaaagactg catcaccagg tcagccgaga
    24121 agcccaggca cgccaggtct ttcagttcat cacgggtcat cgggccggtg gtgtcctggg
    24181 aacctacaga agtcattttc ggttcacagt acgcgcccgg acgaatgcct ttcacgccac
    24241 aggctcggcc caccattttc tgcgccagcg agaagccacg gtcgctctca gcgacatctt
    24301 tcgcctgacg gaacacatcg ctgtgcggca ggccaagtgc ttcacgcgct ttggtggtca
    24361 ggccacgccc gataatcagc ggaatacggc cgccagcacg cacttcatca atcagcacgt
    24421 cggttttcag ttcgaaggtt gccagcagtt cgccggtttc gtggttacgc acttcacctt
    24481 tgtacgggta aacgtcaatt acgtcgccca tgttcaggtt agagacgtcg acttcgattg
    24541 gcagtgcacc cgcatcttcc atcgtgttaa aaaagattgg tgcaatttta ccgccgaggc
    24601 acaaaccacc gccgcgtttg ttcggcacat gtggaatatc atcgcccata aaccacagaa
    24661 cggagttcgt ggcggattta cgcgatgaac ccgtacccac aacgtcaccg acgtacgcca
    24721 gcgggaaacc tttctgttgc agagcttcaa tctgtttgat cgggccaaca acaccaggct
    24781 gatctggctc aataccttca cgggcgtttt tcagcatcgc cagcgcgtgc agtgggatat
    24841 ccgggcgtga ccacgcatct ggtgccggag agaggtcatc ggtgttggtt tcgccagtga
    24901 ctttgaagac ggtaacggtc agtttttcag ccagcgccgg gcgattcagg aaccattcgg
    24961 catccgccca ggactgcatt acctgcttcg catattcgtt gcctgctttg gctttctctt
    25021 ctacgtcata gaagttatcg aacatcagca gcgtgtgaga aagtgctttg gcagcgatcg
    25081 gtgccagttt ggcatcatcc agcgcgtcga tcagcggatg aatgttgtaa ccaccctgca
    25141 tagtgcccag cagttcaatg gctttttccg gagtcagcag cggggattta gcttcgcctt
    25201 tggcgacagc agccaggaag cctgctttga cataggcggc ttcatcgacg ccagggggaa
    25261 cacggttggt taacagatct aacaggaatt cttcttcgcc cgcgggcggg tttttcagca
    25321 gctctacaag tgcggccatt tggtttgcat ccaggggttt gggcgcaatc ccctcagcgg
    25381 cacgctcagc tacgtgctta cggtattctt ctagcacgac ggttctcctc gctctcattg
    25441 tcatagtgcg gcaggcgatt ctcttcacgc tcctgtgaga cagcagtttg tagggtaaat
    25501 gcccagtacc gcaacgggca gaatagcagg attttgacga tgtgttaatc tgtttacaaa
    25561 aaagcaacat aaaaaagtgg ctgaatcgtt aaggatggtc tagagattgt ttccctccgt
    25621 aaaaacttcg ttaattcgca tggtgataat cacctttctc aacgaatccc aagattagtc
    25681 aaaatttaaa ctacggcctc tttatactcg gattcacagc acctgcgggt ggccgttcgc
    25741 cgaccattgc gatttccttg agatccgaat tatgaaaatg actttgccgt ttaaacccca
    25801 tgtgctggca ctaatttgca gtgccgggct ttgtgccgcc tctgccgggc tatatataaa
    25861 aagccgcaca gtggaagcgc ctgtggaagc gcaatcgaca caacagactg cgcctgacat
    25921 ctccgcagtt acgcttcctg caacggtttc cgtgcctcct gtaacacctg ccgtcgtcaa
    25981 atctacattc agcactgcac aaatcgatca atgggtcgcg cccgtcgcgc tgtaccccga
    26041 ctctctgctt tcacaagtgt taatggcatc aacctatccg gcaaacgttg ctcaagcagt
    26101 gcaatggtca cacgataatc cacttaaaca aggcgatgct gctattcagg cggtatctgg
    26161 ccagtcctgg gacgccagcg tcaaatcact ggtggccttt ccacagttga tggcgttgat
    26221 gggcgaaaac ccgcaatggg tgcaaaacct gggcgatgct tttctggcgc agccgcagga
    26281 cgtgatggac tcggtacagc gattgcggca actggcgcaa caaaccggct cgctgaagtc
    26341 atcaactgaa caaaagatca tcacgacaac gaaaaaagtc gtaccggtaa atcagcccgc
    26401 caacgcccct gccacacaat caaataccgt ttctacgtcc agccccgtcg tcgcagaacc
    26461 tgcaccgacc gtaatagcca ttgagccagc caatccggat gtggtttata ttccgaacta
    26521 caacccaaac gtggtttacg gcagttgggc caataccgct tatccgccag tttatctgcc
    26581 gccgccagcc ggagaaccgt ttgttgacag ctttgtgcgc ggattcggct atagcatggg
    26641 cgttgctacc acatacgcac tattcagcag catcgactgg gacgacgacg atcatgacca
    26701 tcatcatcat gacgatgatg attatcatca ccacgatggc ggtcatcgtg acggtaatga
    26761 ctggcaacac aacggcgaca acatcaatat cgacgtcaac aatttcaacc gtatcaccgg
    26821 tgagcatctt actgataaga atatggcatg gcggcacaat ccgaattacc gcgatggtat
    26881 gccttatcat gatcagaata tggcaaagcg gtttcaccaa accgatgtta aaggcggaat
    26941 gagcgccacg ccgctacctg ctccaacacg cgacagccag cgtcaggcgg cagcaagcca
    27001 gtttcagcaa cgaacacacg ccgcccccgt cattacgcga gatacccaac gtcaggcagc
    27061 ggcacagcgg tttaatgaag ctgaacacaa tgggagctat gacgacttcc gcgactttag
    27121 ccgtcgccag ccactgaccc agcaacaaaa ggacgccgct cgtcagcgtt atcagtcggc
    27181 ttctcctgag cagcgccagg cggctcgcga gaaaatgcag actaacccac agaaccagca
    27241 gcgaagagag gcggcacgtc agcgtattca gtccgcgtcg cctgagcagc gccaggcggt
    27301 tcgcgagaaa atgcagacta acccgcagaa tcaacagcaa agagatgcag cacgtcagcg
    27361 tatccagtct gcctcgcctg agcagcgtca ggtttttcga gagaaagttc aggagagccg
    27421 cccgcagcgt ctaaacgaca gtaaccatac tgcccgattg aataatgagc aacggtcagc
    27481 ggtacgcgaa cggttgtctg agcgtggagc aaggagactg gaaaggtaaa ttgcaggcgt
    27541 aaaaaagcgg cgttgttagc cgcttaagac tgatgacaaa catcgaatcg cccgatgcgc
    27601 aagcttatcg ggcctacata agccatgcaa tatattgaat ttgcatgatt ttgtaggtcg
    27661 gataaggcgt ttacgccgca tccggcatca accacgagca ctttatcaac aatctgaagc
    27721 ggccagaata gccgcttctt aatttgcagg atgttccggc aaacgaaaaa ttacttcttt
    27781 ttcgctttcg ggttcggcag gtcggtaatg ctaccttcga acacttctgc cgccaggccc
    27841 acagactcgt gcagagtcgg gtgcgcatgg atggtcagtg cgatgtcttc agcatcacaa
    27901 cccatttcga ttgccaggcc gatttcaccc agcagctcgc cgccgttagt accgacaatc
    27961 gcaccaccga tcacacggtg agattctttg tcgaaaatca gcttggtcat accgtctgcg
    28021 cagtcagaag cgatagcacg accagaagca gcccacggga aggtggcggt ttcgtagcta
    28081 atgcctttct ctttcgcttc tttctcagtc agacctaccc atgcaacttc tggttcggta
    28141 taggcgatgg acgggataac tttcggatcg aagtaatgtt ttttaccggc gataacttca
    28201 gcggcaacgt gaccttcgtg aacacctttg tgtgccagca ttggctgacc gacgatatcg
    28261 ccgatagcaa agatgtgcgg tacgttggta cgcagctgtt tgtcaacgcg gatgaaacct
    28321 cggtcgtcaa cttccacgcc agctttgcct gcgtcgaggt ttttaccgtt cggcacacga
    28381 ccaatcgcta ccagcacggc gtcgtaacgc tgcggttcag cgggtgcttt tttgccttcc
    28441 atcgtcacat aaatgccgtc ttctttcgct tcaacggcgg taactttggt ttccagcatc
    28501 aggttgaatt tcttgctgat acgcttggta aagactttaa cgatgtcttt atcagctgcg
    28561 gggatgacct ggtcgaacat ttcaaccacg tcaatctgtg aacccagcgc gtggtatacg
    28621 gtgcccattt ccagaccgat gataccgcca cccattacca gcaggcgttc tggtacttct
    28681 ttcagttcca gcgcgtcagt ggagtcccag atacgcggat cttcatgcgg aataaacggc
    28741 agttgaatcg ggcgagaacc cgctgcaatg atcgcgttgt cgaagttgat cacggtttta
    28801 ccgttctcac cttcaacttc cagggtgtta gccccggtaa atttacccag accgttgacc
    28861 actttgactt tgcggccttt cgccatacca gccagaccac cggtcagctg attgatcact
    28921 ttctctttcc aggtacgaat cttgtcgata tcggttttcg gttcgccaaa gacgataccg
    28981 tgttcagcca gcgctttggc ttcttcgata acttttgcta cgtgcagcag tgctttagaa
    29041 gggatacagc cgacgttcag gcaaacaccg ccgagggtgt tgtagcgttc tacgattacg
    29101 gtttccagac ctaaatcagc gcaacggaag gcagcggagt aacctgcggg gcctgcccca
    29161 agtaccacga cctgagtttt gatttcagta ctcatcatga cctctatata tttatctccg
    29221 gcgggtctga cgtttttttt atgacgccca ttatccaccg ggtcgttcta tccgtcggta
    29281 ttttacaaaa ttgttaacaa tttttaaaca acaaacggca accgatttgt ctattcgcta
    29341 ataatctcaa tacatt
//
LOCUS       DAZFFE010000053        29007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFFE010000053 DAZFFE010000000
VERSION     DAZFFE010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.053"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1834)
                     /gene="selB"
                     /locus_tag="ACHJ69_002530"
     CDS             complement(<1..1834)
                     /gene="selB"
                     /locus_tag="ACHJ69_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO5015492.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEGKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHTLLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADKDRLVLRDISARNTLAG
                     ARVLMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDIHSHHGWLHLPDHKAGFSAEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLF"
     gene            complement(1831..3222)
                     /gene="selA"
                     /locus_tag="ACHJ69_002531"
     CDS             complement(1831..3222)
                     /gene="selA"
                     /locus_tag="ACHJ69_002531"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO5015493.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSTFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVTKAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3320..3928)
                     /gene="yibF"
                     /locus_tag="ACHJ69_002532"
     CDS             complement(3320..3928)
                     /gene="yibF"
                     /locus_tag="ACHJ69_002532"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO5015494.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(3999..5135)
                     /gene="yibH"
                     /locus_tag="ACHJ69_002533"
     CDS             complement(3999..5135)
                     /gene="yibH"
                     /locus_tag="ACHJ69_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO5015495.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPDA
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5138..5500)
                     /gene="yibI"
                     /locus_tag="ACHJ69_002534"
     CDS             complement(5138..5500)
                     /gene="yibI"
                     /locus_tag="ACHJ69_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO5015496.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVAPQGDVPDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6037..7950
                     /gene="mtlA"
                     /locus_tag="ACHJ69_002535"
     CDS             6037..7950
                     /gene="mtlA"
                     /locus_tag="ACHJ69_002535"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO5015497.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPVVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAAARRVQEMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            8370..9518
                     /gene="mtlD"
                     /locus_tag="ACHJ69_002536"
     CDS             8370..9518
                     /gene="mtlD"
                     /locus_tag="ACHJ69_002536"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO5015498.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGTLPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9518..10105
                     /gene="mtlR"
                     /locus_tag="ACHJ69_002537"
     CDS             9518..10105
                     /gene="mtlR"
                     /locus_tag="ACHJ69_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO5015499.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(10116..10325)
                     /gene="yibT"
                     /locus_tag="ACHJ69_002538"
     CDS             complement(10116..10325)
                     /gene="yibT"
                     /locus_tag="ACHJ69_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO5015500.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRQLYRPKQVEEK"
     gene            10609..10971
                     /gene="yibL"
                     /locus_tag="ACHJ69_002539"
     CDS             10609..10971
                     /gene="yibL"
                     /locus_tag="ACHJ69_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO5015501.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            11530..12213
                     /locus_tag="ACHJ69_002540"
                     /pseudo
     CDS             11530..12213
                     /locus_tag="ACHJ69_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12257..17026
                     /locus_tag="ACHJ69_002541"
     CDS             12257..17026
                     /locus_tag="ACHJ69_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO5015502.1"
                     /translation="MNKIFKVIWNPATGSYTVASETAKSRGKKSGRSKLLISALVVGG
                     LLSSGVQASTALDGGGASESTPLTDTWIAIGQGAKASSVDTSPSGGPGTSSIAIGQEA
                     QSGIGSTSLGFKSQATGERSVALGQTADATGNRAIAIGSGAKSTADYSLTLGNGSKAF
                     AENATAIGKDARAEGKGSQAFGKVALATGENAIALGSNSNASGDNAVALGSTSQAYGD
                     SSLAIGELSETNAENAIALGKLSNASKINSIALGSNSTASGVGSVALGENSFAGGINS
                     LALGSQSNANGDNAVALGVGSVAEQDNTVSVGNSTTQRKITNMAAGLILPGSTEAING
                     SQLHALSASVAARLGAGAGVNGDGSINAPSYKLKSGTYNNVGEALLGIDNDTLHWDKT
                     EGAFSASYIVKNADGSPKSSIAQNKIINVAKGTISATSTDVVNGSQLYDLQQDALLWN
                     GTAFSAAHGSDTTSKITNVAKGTVSATSTDVVNGSQLYDLQQDALLWNGTAFSAAHGT
                     EATSKITNVEDGTISDTSKDAVNGSQLKETKDDVATNTANIATNTTDIATNTANIADN
                     TADIATNTTDIATNTANIATNTTDIATNTADIATNASSITTLNADVDTLEKDALLWNG
                     TAFSAKHGTEATSKITNVTAGDLTAGSTDAVNGSQLKTTNDNVSTNTTNITNLTDSVG
                     DLKDDSLLWNKTAGAFSAAHGTEATSKITNLLAGKVSSDSTDAINGSQLYGVADSFTS
                     YLGGGADISDTGVLTGPTYTIGGTDYTNVGDALAAINTSFSSSLGDALLWDATAGKFS
                     AKHGINNDPSVITDVANGAVSSTSSDAINGSQLYDVSDYIADALGGNAVVNADGSITT
                     PTYAIAGGSYDNVGDALEAIDTTLDDALLWDETANGGNGAFSAAHGKDKTASVITNVA
                     NGAVSATSSDAINGSQLYSTNKYIADALGGDAEVNADGTITAPTYTIANTDYNNVGEA
                     LDALDNNALLWDEDAGAYNASHDGNASKITNVAAGDLSTTSTDAVNGSQLNATNILVT
                     QNSQMINQLAGNTSETYIEENGAGINYVRTNDSGLAFNDASAAGIGSTAVGYNSVADG
                     DNSVAIGHGSNSIVETSIALGSESVSSRVIVKGSRNTSVSEEGVVIGYDTTDGELLGA
                     LSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYYHANSTAEDSLAVG
                     EDSLAMGAKTIVNGNAGIGIGLNTLVLADAINGIAIGSNARANHADSIAMGNGSQTTR
                     GAQTNYTAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDAQVS
                     QNTQSITNLNTQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGADANAQGKDSVAI
                     GSGSIAAADNSVALGTGSVADEENTISVGSSTNQRRITNVAAGVNATDAVNVSQLKSS
                     EAGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDAVNYAQLKQSVQETK
                     QYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAV
                     ALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            17438..19093
                     /gene="lldP"
                     /locus_tag="ACHJ69_002542"
     CDS             17438..19093
                     /gene="lldP"
                     /locus_tag="ACHJ69_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO5015503.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGSSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            19093..19869
                     /gene="lldR"
                     /locus_tag="ACHJ69_002543"
     CDS             19093..19869
                     /gene="lldR"
                     /locus_tag="ACHJ69_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO5015504.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            19866..21056
                     /gene="lldD"
                     /locus_tag="ACHJ69_002544"
     CDS             19866..21056
                     /gene="lldD"
                     /locus_tag="ACHJ69_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO5015505.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQVLGKELPAALAPMAK
                     GNAA"
     gene            21104..21577
                     /gene="trmL"
                     /locus_tag="ACHJ69_002545"
     CDS             21104..21577
                     /gene="trmL"
                     /locus_tag="ACHJ69_002545"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO5015506.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(21630..22451)
                     /gene="cysE"
                     /locus_tag="ACHJ69_002546"
     CDS             complement(21630..22451)
                     /gene="cysE"
                     /locus_tag="ACHJ69_002546"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO5015507.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(22531..23550)
                     /gene="gpsA"
                     /locus_tag="ACHJ69_002547"
     CDS             complement(22531..23550)
                     /gene="gpsA"
                     /locus_tag="ACHJ69_002547"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO5015508.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERERCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(23550..24017)
                     /gene="secB"
                     /locus_tag="ACHJ69_002548"
     CDS             complement(23550..24017)
                     /gene="secB"
                     /locus_tag="ACHJ69_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO5015509.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(24080..24331)
                     /gene="grxC"
                     /locus_tag="ACHJ69_002549"
     CDS             complement(24080..24331)
                     /gene="grxC"
                     /locus_tag="ACHJ69_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO5015510.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(24472..24903)
                     /gene="yibN"
                     /locus_tag="ACHJ69_002550"
     CDS             complement(24472..24903)
                     /gene="yibN"
                     /locus_tag="ACHJ69_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO5015511.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            25148..26692
                     /gene="gpmM"
                     /locus_tag="ACHJ69_002551"
                     /gene_synonym="pgmI"
     CDS             25148..26692
                     /gene="gpmM"
                     /locus_tag="ACHJ69_002551"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO5015512.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFTALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            26726..27985
                     /gene="envC"
                     /locus_tag="ACHJ69_002552"
     CDS             26726..27985
                     /gene="envC"
                     /locus_tag="ACHJ69_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO5015513.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALSERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGSPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            27989..28948
                     /gene="yibQ"
                     /locus_tag="ACHJ69_002553"
     CDS             27989..28948
                     /gene="yibQ"
                     /locus_tag="ACHJ69_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO5015514.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPLEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(28935..>29007)
                     /locus_tag="ACHJ69_002554"
     CDS             complement(28935..>29007)
                     /locus_tag="ACHJ69_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015515.1"
                     /translation="RLWQWRDKTRSHHRITRSAFNLR"
BASE COUNT         7178 a         7652 c         7511 g         6666 t
ORIGIN      
        1 gaaataataa tgcgtcgcgc aataaatgat catttccacg acgacgcgta aagccaatac
       61 ggtcaaaata ttccagaatc tgaattgcca gcttgcggcc tacgcctaag cgatcgcgga
      121 aatccgccgc gcaggttgaa ccgcattcct gatcgagatc gcggatcata ttggcaaatt
      181 cgacaatccg atcgttacgg taataacgat ctttgacgat cgcagtaatt atcccctgtt
      241 gcgccgcctg gcgtagcgtc aggcgcattg cctgctcgtc ggttcccgtc tcttttgcca
      301 ggtcacgcac ccaccacggc tcgtcaccaa acagcggctc tgctttttgc cagatggcct
      361 gctgctctgc actgaagccc gctttatgat ccggcagatg cagccagccg tgatgactgt
      421 ggatgtcgcc gctttcgcgc atcttttcaa tcagcaacag taccagcgct tcatcttcca
      481 ttggcaacgc catacgtcgc agacgttcgc gcccaggacc aggttcatcg cgatgttgct
      541 catgataagt cgctaatgtg tcgagaattt tccgctgcca gcgggcggca actggcgcat
      601 tcaacaagct gtaaccagcc tgaatataac caggctgttg cagcagttcg cgcatccctt
      661 cgccgttgag ctgccgtgcc caggcgaaat ccgcaaggtt aaccgcgccg cgttccagat
      721 gaacagataa cgcatcggca tcgctctgcg cccgtgcaag agacgccagc cattgcagat
      781 attccggctt acgcttaccg cggcgcggcg ggttaagcat caacacacgc gcccccgcca
      841 gcgtgttgcg ggccgagata tcgcgcagca ctaagcgatc tttatccgcc agccacagcg
      901 gcgtgtcgaa caccagctcg gcaaggttat cttccagcag cgaaacgcgt cccgtaacgt
      961 ggctggcggc gtggtgaata tgcagcggct gccactgggt cagcggtgta tgggtttgaa
     1021 gctcgacaat cacccgtgtg aacggctccg gcggcacatc ggcaagcagc cagtcgccac
     1081 ggttaatctg ctctttttcc gcatcacccg cgatgttaag cgcgatacgc tgcccggcat
     1141 gggcagtttc tgttggctga ttttgcgcat gcagcgcacg cacgcgcatc ggtttattta
     1201 caccagtcag ccagagtgaa tcgcctactt tcacttcccc gcttaacgcc gtaccggtga
     1261 cgaccagccc ggcacctttt acggtaaatg cgcggtcgat cgcgaggcgg aaactatgtt
     1321 ggctggcgtg ttcacgttct ggtaactgaa gcagatgctc gcgcagggca tcaattcccc
     1381 gaccttcggt tgctgcggtg ataaacagtt ttgcctcagc aaaaccgtat tcccgcagca
     1441 cctcctttac ctggcgttca acctcatcaa cacgcgcttc gtccacgcga tcggctttgg
     1501 tcagcgccac tgtcagcatc gggttaccgg tcagctgcaa aatcgccaga tgctcacggg
     1561 tctgtgccat cacaccgtca tcgcacgcca ccaccaacag cgtgtgatcg ataccaccaa
     1621 cgcccgccag catgttggaa agaaactttt catgaccggg cacgtcgata aaaccaggca
     1681 cgcgaccatc cggctgcggc cagtaggcat agccgagatc gatggtcatg ccgcgctttt
     1741 ttccttccgg cagacggtcg gcatttacgc ccgtaatcgc ctgcaacaag gttgttttgc
     1801 cgtggtcaac gtgtccggca gtcgcaataa tcatttcaac aacatctcca aaaaccgttg
     1861 ctcatcttca aggcagcgta aatccagcca caatcgtccg tcataaatac gaccaatcac
     1921 cggcactggc aattcacgcc agcgggcggc taatgactca aggtggctac cgcgtccatc
     1981 atggggggta aacgttaatg ccgcgctcgg caggcgatca accggcagcg aaccactgcc
     2041 gatctgcgaa agacatggca taacctgtac cgcaaactcc gcgccgtaat gtgcggcaag
     2101 gggagcctgt aaacgttgtg cctggatttg aatgacctct gcgctgcggg taagcaggcg
     2161 cagggtcggt aatttttcac tcagagcttc agggtgtaaa taaagacgca acgtggcttc
     2221 cagcgccgcg agggtcattt tatccgcgcg taatgcacgc ttcagcgggt ggctttgcag
     2281 gcgggcgatc atttcttttt taccaacaat aattcctgcc tgcggcccgc ctaacagctt
     2341 gtcgccggag aagctcacca gactgacgcc cgccgcaatc aactcctgcg gcattggctc
     2401 tttcggcaaa ccgtactggc taagatcgac cagcgagcca ctgcctaaat cagtcaccac
     2461 tggaacatcc agttctttgc cgagcgccac cagttccgct tcatctatcg ctttggtgaa
     2521 cccctgaatg ctgtagttac tggtatgtac tttcatcaac agtgcggtat tttcattcac
     2581 cgcctgacga taatcattcg cgtgcgtgcg gttggtggtc cctacttcgt gtagggtgca
     2641 tcctgcctga cgcataacat cgggaatacg aaacgcgccg ccaatctcca ccagttcgcc
     2701 gcgagaaacc accacctctt tcccgctggc agtggccgcc aacatcaata acaccgccgc
     2761 cgcattgtta ttaacgatac aggcatcttc cgcccccgta atacggcaca gcagctgcgc
     2821 cagcgcccga tcgcgatgtc cgcgtccggc gtcgtccaga tcgtactcga gggtcactgg
     2881 cgaacgcata gccttcgtaa cggcttccac cgcggcttcc gcctgtaaag ctcgcccaag
     2941 gttggtatgc agcacggttc ctgtcaggtt gattaccgga cgcagcgcgc tttgcgcctc
     3001 tttcgtcaac cgggcatcga cttcttgcgc ccagttttca caccacgcgg gcagcgtctg
     3061 gctgtcacga atcacttctc gcgcttcgtc aagcatctga cgcagcaatt ccaccacgcg
     3121 ggtgtgacca taagtatcac gcaaagaaag gaaggtgcta tcgcgcaata agcgatcaat
     3181 agccggaagt tgactataga gggaacgcgt ttcggttgtc ataggaaacc tggctgatca
     3241 aggccctctc acacggagaa gggcgtttaa cataaccacg gattgtaacg tgagatgggt
     3301 caggaggaca tatcgcgcat caagcctttg gcggttcggt gcgagcaaaa ctttcgcggc
     3361 taaacaggtt ttcgaccagt ttgactaagt gtgggcggtc gacacaccag cctggcgcaa
     3421 cgcggcggaa attgagatat ccgacggcac aggcaatggc gatagtcgcc agattgaccg
     3481 tatcggtttt gagcgtgcca tcgaccagat atccttccag cacatccaga ctgcggttga
     3541 ttttctcacg ctgacgtaac aattcatctt cagactgctg cgccgccgga cgcgcctgtt
     3601 cacgcaccga taccagcccg gcatccataa tgccatccgc cagtgcctca attttgcgca
     3661 cccgcaacga ctccagcgga tcgcgcggca acatcgccgg agcaacattc attaattcaa
     3721 tatattcagc gatgatcggt gaatcaaacc agcattcgcc ctcttcggtc accagcaccg
     3781 gcacttttcc taacgggtta aattgcgcca cgccgttatc cgcgttatag ggcagttcat
     3841 taataaattc gaacgttatg cccttttcta acaacagaat agaaagtttg cgtacaaacg
     3901 ggctggtgta gctaccgacg agtttcatgc cgagtccttt gtgcgaggaa aaatatcagt
     3961 atggctcagg ctcgcgcaaa acacaggtga tacaagtctc aatgatccaa ataaagataa
     4021 tgcatccaac tggtcattct tagcagcact ttccgcatca ccgaaacatg gctgaaatgg
     4081 tcggagtaaa ctgccacctg ggcgtagatg ccgtcgggta aggcatcgat atcagcgtca
     4141 gggtccagtt caatggttcc cagcacaccg tccgtgccgg gcacgaccgt taatgattgc
     4201 aataccccct gcgcctgata agaaccgcct ggcacgacag gtaaaatact ggtcagtttg
     4261 ccgtgaaaca cctgcccagg tagcgcgtta aacaccactt ccgcatcatc gccgggtttc
     4321 agacgtaaca gcgagttctg ccgaaattgg gcgacaattt gccgtttttg ctcggggatg
     4381 aaaaccatca ccggacgtag cggcaaggca gctgcgtatg tacctgggcg gatcagtacc
     4441 tgagtgacgt agccattgct cggtgcgcga atgacggtct gctcaaggtt atattttgct
     4501 tcagtaagtt gcgctcttaa gctcacaatc tgcgattgct cgccgttaac catactgtcg
     4561 agctggctct ggatctgcgc ctgctccgcc accgagcctt tcaccagcgc atcctgcgcg
     4621 aggaaatttt gccgcgcatc gtcgatgtca cgttccgaga acggattcac cgccgcctgg
     4681 ctgcctttca ggtaacgttg ataattttta aacagacggt cgcgctccgc tgaaacctgg
     4741 gtggtgttgg cctgcgcttc agtgagctgc gcgcgcagcg tctttatatt atgcgtcgcc
     4801 gtcatcaggt cagcctgaag cctgtcaact cgcgcctggt aacgaaccgg gtcgagctta
     4861 aaaagcacct cgcccttctg aataagctga ttatttttgt cggtgacttc agtaacaatt
     4921 cccgtcacct gtggcgtgat ggggatcgct atcactgcct tttgcgcggt aaaagtgtaa
     4981 gggtggttgt agttcatcaa caaaatcaaa ccactcacca gaaacacgcc tcccagcgcc
     5041 gccgtcgcca gcgtccactg attcaccgga atgcggaaga ttttaaagac cgcccacgcc
     5101 agcgccacgt aagttaaaac aatcaataga tccataatta gatctccgga aacgtggaat
     5161 agtcagtctt tttctcggcg gcgagttggt gctccagccg ggaaatccgg tcagaaagtg
     5221 cggcgatttc cgggtctggc acgtcgcctt gcggcgcaac atgtgactgc atgccccagc
     5281 cgcgctccgg ttgatagagc gtcgcccaga tccacaggaa cggccagata acatgcagag
     5341 taaacaggct cacccagccc gccgtatgaa tagcgtcggc atggggatgg ttgcgctttt
     5401 tggcaatcag ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga
     5461 cgaagatcag cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt
     5521 tatcagtgtt gttttttggc tggcagccag aagggagtca ggctgatatt ttgacaataa
     5581 tccgggttcg cgattctcgc cataacacca aagaataatt ttaagaggtg atgagttgct
     5641 tagttacata acgatttcat gacgaaggca taacatgctg tagatcacat caggtgaacg
     5701 ccgtaagaaa atatcttgtg attcagatca caaagattca acaaatcatc aaaataaaaa
     5761 tgtgacatca ctcacattta aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt
     5821 tacacaagca ttttgtgatg agcgtcacgt caattacctc tctaccccct acatttatgt
     5881 gactgatatc acacaaaagg ccaccgactg gacagttaac cgattcagtg ccagatttcg
     5941 cagtatctac aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca
     6001 gcctgcgcga cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa
     6061 gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc
     6121 gcgtggggta tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgaaacgctg
     6181 gcgaagctgg tcgggccgat gatcacttat ctcctgccgc tgctgatcgg ttataccggt
     6241 ggtaagctgg taggcggcga acgtggcggc gtagtcggtg ccatcaccac catgggcgtt
     6301 atcgtcggcg cagatatgcc gatgttcctc ggctctatga ttgcaggtcc gctgggcggc
     6361 tggtgcatta agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgaaatg
     6421 ctggtgaata acttctccgc aggcatcatc gggatgatcc tcgctattct ggcatttctc
     6481 ggcattggcc cggttgttga agccctgtct aaaatgctgg cggcgggcgt taacttcatg
     6541 gttgtccatg acatgctgcc gttggcgtct atctttgttg aaccggcgaa aatcctgttc
     6601 ctcaacaacg ctattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa
     6661 ctgggtaaat ccatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg
     6721 ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc
     6781 atccacttcc tggggggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt
     6841 ctgattctgg ccgttatcct cggcggtatg actggcgtgt tcacgctaac tatcctgggc
     6901 ggtggtctgg tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca
     6961 aaaggtgcat acttcgctaa catcgcgggt gtgtgtgcgg cgatggcagt ctccttcgtt
     7021 gtctctgcca ttctgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagctgct
     7081 gcacgtcgcg ttcaggaaat gaaagctgag tctaaaggcg catctccgct gtctgcgggc
     7141 gatgtgacta acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga tgctggtatg
     7201 ggttccagtg cgatgggtgc aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtcg
     7261 cagatttcgg tcactaacag cgcgatcaac aacctgccgc cagatgtgga cctggtcatc
     7321 actcaccgtg acctgaccga acgcgctatg cgccaggttc cgcaggcaca gcatatttcg
     7381 ctgaccaact tcctcgacag cggcctgtac accagcctga ccgaacgtct ggttgctgcc
     7441 cagcgccata ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag ctttgacgat
     7501 tccagtgcta atctgttcaa actgggcgcg gagaacatct tcctcggtcg caaagcggcc
     7561 accaaagaag aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag
     7621 ccggaatacg ttcaggcgat gttggatcgt gaaaaactga ccccgactta tctgggtgag
     7681 tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtc
     7741 gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt
     7801 ctggtgattg gtattgctgc ccgtaacaac gagcacattc aggttatcac cagcctgacc
     7861 aatgcactgg atgatgagtc cgtcatcgag cgtctggcac acaccaccag cgtggatgaa
     7921 gtgctggaat tgctggcagg tcgtaagtaa tccattccca ccctctccgc atgaagaagg
     7981 tggggctaat tgcctgatgc gcttcgctta tcaggcctac aacataaagc acaatttgtt
     8041 ttgtcggtcg gataaggtgt ttacgccgca tccggcagtc ggtgcacaat gcctgatgcg
     8101 acgctgacgc gtcttatcag gcctacaggt cagtgccaaa cacgtaggcc ggataaggcg
     8161 tttacgccgc atccggcagt cggtgcgcaa tacctgatgc gacgctgacg cgtcttatca
     8221 ggcctacagg tcagtgccaa acacgtaggc cggataaggc gtttacgccg catccggcaa
     8281 aaagcccctc tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc
     8341 gggtaaaaac attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata
     8401 tcggtcgtgg ctttatcggt aaactgctgg cggacgcggg tatccaactg acgtttgccg
     8461 atgtaaatca ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg
     8521 ttggtgaaac cgagcaggta gataccgttt ccggcgtcaa tgccgtcagc agcattggcg
     8581 atgatgtcgt tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggccctgtcg
     8641 tgctggaacg tattgctccg gccatcgcca aagggctggt gaaacgtaaa gaacaaggta
     8701 atgaatctcc gctgaacatc atcgcctgtg aaaacatggt ccgcggcacc acgcagctga
     8761 aaggccatgt gatgaacgcc ctgccggaag atgccaaagc gtgggtagaa gaacacgttg
     8821 gctttgttga ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc
     8881 cgctggaagt gacggtagaa accttcagcg aatggattgt cgataaaacg cagttcaaag
     8941 gcacgctgcc aaacatccct ggcatggaat taaccgacaa tctgatggca tttgtcgaac
     9001 gtaaactctt caccctgaat acaggtcatg ctataaccgc gtacctcgga aaactggccg
     9061 gtcatcagac cattcgtgac gcaattctcg acgagaaaat ccgcgcggtg gtaaaagggg
     9121 caatggaaga aagcggcgcg gtactgatca agcgctacgg ctttgatgct gacaaacatg
     9181 cggcatacat ccagaaaatc ctcggtcgtt tcgagaaccc gtacctgaaa gatgatgtag
     9241 aacgcgtagg ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccac
     9301 tgctcggaac cctggaatat ggtctgccac ataaaaacct gattgaaggt attgccgctg
     9361 caatgcactt ccgcagtgaa gatgacccgc aggctcagga actggcagcg ctgattgccg
     9421 acaaaggccc acaagccgcg ctggcacaga tttccggtct tgatgccaac agcgaggttg
     9481 tatccgaggc ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggatac
     9541 cctgcgcccg aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt
     9601 tgaaaaccgt gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac
     9661 cgccgtcgag cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga
     9721 cgattacgcg gtgaagtatg ctgtagaacc gttactcgac ggcgatggtc cgctgggcga
     9781 tctttctgtg cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aagaatacga
     9841 agatgcggaa ctgctgatgg cattgcgtga agagctaaat cacgacggca acgagtacgc
     9901 ttttaccgac gacgaaatac ttggaccctt tggtgaactg cattgcgtgg cggcgttacc
     9961 accgccgcca cagtttgaac ctgcagactc cagtttgtat gcaatgcaaa ttcagcgtta
    10021 ccaacaggct gtgcgatcaa caatggtcct ttcactgaca gagctgattt ccaaaatcag
    10081 cttaaaaaaa gcctttcaaa agtaagtact ttcgcttact tctcctctac ctgtttcgga
    10141 cgataaagct gacgataata ctccagacgt tgtagatata acggcacgct ttcatcaggt
    10201 attccggacg gaatcgcgtt acggggaggt agttttttca gatactcccg gaacgcctgg
    10261 ctggatgcca tgaaatctac ggctttatcg ataagaagcg gaacgttttc acctaatttg
    10321 cccatgatga tatctccgta ttaccccgcg ccgggaatgc gcggccgcca attttagttt
    10381 agatcccggt ataaatgaca ttaaggaaaa cgtgctcaat cctcaataat cacctgcttc
    10441 tgttgactat cttttttctt taaatttctt aactaatcaa tgtattgggt gattttttaa
    10501 cgcattaatg gttacaatct ggaactatta caatgccatc agttgtgaac aaagcacctg
    10561 gtcgcgcata ctagaggcta taaatttatc tctatcagaa gtcatcacat gaaagaagtc
    10621 gaaaaaaacg aaatcaaacg tctcagcgat cgtctggacg ccatccgtca ccagcaggcc
    10681 gatctgtcgc tggttgaagc cgcagacaaa tatgccgaac tggaaaaaga gaaagccacg
    10741 ctggaagcag aaattgctcg cctgcgcgaa gttcacagcc agaaactcag caaagaagcg
    10801 cagaaactga tgaagatgcc gttccagcgt gcaatcacca aaaaagagca agctgatatg
    10861 ggcaaactga aaaaaagtgt tcgcggactg gtggttgtgc acccaatgac cgcgctgggc
    10921 cgtgaaatgg gcctgcaaga gatgacaggg ttctcaaaga ctgcgtttta agttcctttc
    10981 tcgtcgctgt aatcacagcc tctcaccatt tactcgctcc tgctggcaca tgcaggagcc
    11041 tgtttcagag atcgaatctt tataacgttc ttaacattat atattcaact ccacctacac
    11101 ttctgagcga tcgtagcagg ctaaattctg aaaatcaggt acacattaaa gaatgatgta
    11161 tcaatatcaa caatgaattt ccgcatatca atgctcgaaa cataattaat tatttaaatt
    11221 tttcaatcag cttcgcaaat tccacaataa aacatcactc aactgaatat ttacctttcc
    11281 ctgccctgtg cctttatccg gtgaaacatt tgacatcgat tgacaccgat cattaaaatc
    11341 cgcgcgatta atattattgg ctacaaactg aaatcgtttg acatttaata acatattcgc
    11401 acaaccaatt aatacaaaaa ctctctaaca aatttatttt gcggatatgc ctcattccaa
    11461 tcattcataa aaaaaatgat tgtgccgtaa ttatcaactg tatcgggatt atgaaaagga
    11521 attcatccta tgaacctaaa gaaaacactg ttaagcgtgt taatgatatt gcaactttgc
    11581 ttattggtag ggtgtgacta tattgaaaaa gcgagtaagg tcgacgatct cgttacacag
    11641 caagagttac aaaaaagcaa aattgaggcg cttgaaaaac aacaagaact cgacaagcgc
    11701 aagatagaac actttgaaaa acaataaact accatcataa acagtaccaa aacgctcgct
    11761 ggcgtgatga aggcagttaa agacaaacag gacgaatttg tctttacaga atttaacccg
    11821 gcacaaaccc aatacttcat tttaaataac ggttctgttg gtttggcagg gaaaatactg
    11881 tctattgacg cagtagaaaa cggcagcgtt attcgtattt cactggttaa cttattaagt
    11941 gttcctgtat caaatatggg tttttacgcg acatgggggg gagaaaaacc caccgacatc
    12001 aacgcattag caaaatggca gcaattgcta tttagtacct caatgaactc ctccctgaaa
    12061 ttattaccag gtcaatggca agacattaat ttgacgctaa aaggtgtctc gcccaacaac
    12121 ctcaaatatc tgaaattagc catcaacatg gcaaatattc agttcgaccg tcttcaacct
    12181 gctgaatctc cacagcggaa aaacaaaaaa taaatgcagc ttctgcgctt atatcaagga
    12241 atagagagca tcaataatga acaaaatatt taaagttatc tggaatccgg caacaggtag
    12301 ttacaccgtt gccagtgaaa cagcaaaaag tcgtggtaaa aaaagcgggc gcagtaagct
    12361 gttaatttct gcgttggttg tgggtgggtt gttgtcgtct ggagtacaag catcgaccgc
    12421 gcttgatggt ggtggggcta gcgaaagcac tccattgacc gatacctgga ttgccattgg
    12481 tcagggtgct aaagcatcat cagtagacac ttcaccatca ggaggaccag gaacatcaag
    12541 tattgcaata gggcaagaag cccaatctgg gataggtagt acctcattag gtttcaagag
    12601 ccaggctaca ggagaaagat ctgttgcttt aggacaaaca gctgacgcaa ccggtaatag
    12661 ggccatagca atcggtagtg gagctaaatc gactgccgat tattccctta cgctggggaa
    12721 tggatctaag gctttcgctg aaaacgccac ggcgataggc aaggacgcaa gagcggaggg
    12781 caaaggtagt caggccttcg gaaaagtggc cttggccact ggggagaatg ccattgcctt
    12841 gggtagtaac agcaatgcat ctggtgataa cgccgttgcg ctgggtagta caagtcaggc
    12901 atatggcgac tccagccttg caataggtga gttaagtgag accaatgctg aaaatgctat
    12961 tgcactggga aaactcagta acgcaagtaa aatcaattca atcgccctgg gtagtaacag
    13021 cactgcatct ggtgtgggct ccgttgcgct gggagagaat agttttgctg gaggcataaa
    13081 cagtcttgct ttgggtagtc aatccaatgc aaacggtgat aacgcagttg ccctcggtgt
    13141 aggatctgtc gcagagcaag acaataccgt ttcggtaggg aacagcacaa cacaacgcaa
    13201 aattaccaat atggctgccg gtcttatact accaggcagt actgaggcca ttaacggttc
    13261 acaacttcat gcattaagtg cctcagtcgc agcccgactc ggggctggtg ctggcgtaaa
    13321 tggagatgga agtataaatg cgccaagcta caagctgaaa agcggcacct acaataatgt
    13381 gggagaggcg cttttgggaa tcgacaatga cactctgcac tgggacaaaa ccgagggagc
    13441 atttagtgcc agctatatcg tcaaaaacgc tgatggcagc ccaaagagca gcatagccca
    13501 aaacaaaatc attaacgttg caaagggtac tatttccgcc accagcaccg atgtagtaaa
    13561 cggctctcaa ttgtacgacc tgcagcagga tgctctgttg tggaacggca ctgcattcag
    13621 cgccgcacac ggtagtgata ccaccagcaa aatcactaat gttgctaaag gtacggtttc
    13681 tgcaaccagc accgatgtag taaacggctc tcaattgtac gacctgcagc aggatgcgct
    13741 gttgtggaac ggcacagcat tcagtgccgc acacggcacc gaagccacca gcaaaatcac
    13801 taatgttgaa gatggtacta tttcagacac cagtaaagat gcggttaacg gttcccagtt
    13861 gaaagaaacc aaagacgatg tggccaccaa cacagccaat atcgccacta acaccacgga
    13921 tattgcaacc aacactgcca atatcgccga taacactgct gatatcgcca ctaacaccac
    13981 ggatattgca accaacactg ccaatatcgc cactaacact acggatattg cgactaatac
    14041 cgccgatata gctacaaacg ctagcagtat cactactctg aacgcggacg ttgataccct
    14101 ggaaaaagat gccctgctct ggaatggcac agcattcagc gccaaacacg gcaccgaagc
    14161 taccagcaag atcaccaatg ttaccgctgg cgacctgact gctggcagca ctgacgccgt
    14221 taatggctcc cagctcaaaa ccaccaacga taacgtgtcg accaacacca ccaacatcac
    14281 caacctgacg gattccgttg gcgaccttaa ggacgattcc ctgctgtgga acaaaacggc
    14341 tggcgcattc agcgccgcgc acggcaccga agccaccagc aagatcacca acttactggc
    14401 tggcaaggta tcttctgaca gcaccgatgc cattaacggc tcacaacttt atggcgtagc
    14461 ggattcattt acgtcatatc ttggtggtgg tgctgatatc agcgatacgg gtgtattaac
    14521 cgggccaacc tacactattg gtggtactga ctacactaac gtcggtgatg ctctggcagc
    14581 cattaacaca tcatttagct catcactcgg cgacgcccta ctctgggatg caaccgcagg
    14641 caaattcagc gccaaacacg gcattaataa tgatcccagt gtaatcactg atgttgcaaa
    14701 cggtgcagtc tcgtccacca gcagcgacgc cattaacggt tcgcaacttt atgacgttag
    14761 tgactacatt gccgatgctt tgggcgggaa cgctgtggtg aatgcagacg gcagtatcac
    14821 tacaccgact tatgccattg ctggcggcag ttacgacaat gtcggtgacg cgctggaagc
    14881 gatcgatacc acgctggatg atgctctgct gtgggatgag acagccaatg gcggtaatgg
    14941 tgcatttagc gccgctcacg ggaaagataa aactgccagt gtaatcacta acgtcgctaa
    15001 cggtgcagtc tctgccacca gcagcgatgc cattaatggc tcacagctct atagcaccaa
    15061 taagtacatc gctgatgcgc tgggtggtga tgcagaagtc aacgctgacg gtactatcac
    15121 tgcgccgact tacaccattg caaataccga ttacaacaac gtcggtgaag ccctggatgc
    15181 gctcgataat aacgctctgc tgtgggatga agacgcaggt gcctacaacg ccagccatga
    15241 tggcaatgcc agcaaaatta ccaacgttgc ggctggtgat ctctccacaa ccagtaccga
    15301 tgctgttaac ggttcccagt taaacgcaac caatatcttg gttacgcaaa atagccaaat
    15361 gattaaccag cttgctggta acaccagtga aacctacatc gaagaaaatg gtgcgggtat
    15421 taactatgta cgtaccaacg acagcggctt agctttcaac gatgccagcg cagcaggtat
    15481 tggctctacc gctgtgggtt ataactctgt tgccgatggc gataacagcg tggcaatcgg
    15541 tcatggcagc aacagcatcg ttgagacgag tattgcgctg ggtagcgaat cagtctccag
    15601 ccgtgtgatt gttaaaggtt ctcgtaacac cagcgtatcg gaagaaggtg ttgtgattgg
    15661 ttatgacacc acggatggcg aactgcttgg cgcgttgtcg atcggtgatg atggtaaata
    15721 tcgtcaaatc atcaacgtcg cggatggttc tgaagcccat gacgctgtta ctgttcgcca
    15781 gttgcaaaac gccattggtg cagtagcaac cacaccaacc aaatactatc acgccaactc
    15841 aacggctgaa gactcactgg cagtcggtga agactcgctg gcaatgggcg cgaaaaccat
    15901 cgttaatggt aatgcgggta ttggtatcgg cctgaacact ttagttctgg ctgatgcgat
    15961 caacggtatt gctatcggtt ctaacgcacg cgcaaatcat gccgacagca ttgcaatggg
    16021 taatggttct cagactaccc gtggtgcgca gaccaactac actgcctaca acatggatgc
    16081 accgcagaac tctgtgggtg aattctccgt aggtagcgaa gacggtcaac gtcagatcac
    16141 caacgtcgca gcaggttcgg cggataccga tgcggttaac gtgggtcagt tgaaagtaac
    16201 ggacgcgcag gtttcccaga atacccagag cattactaac ctgaacactc aggtcactaa
    16261 tctggatact cgcgtcacca atatcgaaaa cggcattggc gatatcgtca ccaccggtag
    16321 cactaagtac ttcaagacca acaccgatgg cgcagatgcc aacgcgcagg gtaaagacag
    16381 tgttgcgatt ggttctggtt ccattgctgc cgctgacaac agcgtcgcac tgggcacggg
    16441 ttccgtagca gacgaagaaa atacgatctc tgtgggttct tctaccaacc agcgccgtat
    16501 caccaacgtt gctgccggtg ttaatgccac cgatgcggtt aacgtttcgc aactgaagtc
    16561 ttctgaagca ggcggcgttc gctacgacac caaagctgat ggctctatcg actacagcaa
    16621 catcactctc ggtggtggta acggcggtac gactcgcatc agcaacgttt ctgctggcgt
    16681 gaacaacaac gacgcagtga actatgcgca gttgaagcaa agtgtgcagg aaacgaagca
    16741 atacaccgat cagcgcatgg ttgagatgga taacaaactg tccaaaactg aaagcaagct
    16801 gagtggtggt atcgcttctg caatggcaat gaccggtctg ccgcaggctt acacgccggg
    16861 agccagcatg gcctctattg gtggcggtac ttacaacggt gaatcggctg ttgctttagg
    16921 tgtgtcgatg gtgagcgcca atggtcgttg ggtctacaaa ttacaaggta gtaccaatag
    16981 ccagggtgaa tactccgccg cgctcggtgc cggtattcag tggtaatcat ccattaacga
    17041 atgggtaaac gccacattgc ctgatgcgct acgcttatca ggcctacggg gtgattcagc
    17101 actaccctgt aggccggata aggcgttcac gccgcatccg gcaagataag gcgcaccaga
    17161 tcaacaatct gagggcaatt ctctgatggg aattgccctt ttcttcatta cctctctccc
    17221 aggattggcc ctaccaattc ttcattgatc tgacctctgg ttcacaattc cccaattaaa
    17281 actcacatca ccgttgccaa tacataacat ttggttaact attcattgtc attatcccta
    17341 cacaacacaa ttggcagtgc cacttttaca caacgtgtga caaggagatg agcaacagac
    17401 tcattacacg gcgtgtgtgg actgcaggag acctgcaatg aatctctggc aacaaaacta
    17461 cgatcccgcc gggaatatct ggctttccag cctgatagca tcgcttccca tcctgttttt
    17521 cttctttgcg ctgattaagc tcaaactgaa aggatacgtc gccgcctcat ggacggtggc
    17581 aatcgccctt gccgtggctt tgctgttcta taaaatgccg gtcgctaacg cgctggcctc
    17641 ggtggtttat ggcttcttct acgggttgtg gcccatcgcg tggatcatta ttgcagcggt
    17701 gttcgtctat aagatctcgg tgaaaaccgg gcagtttgac atcatccgct cgtctattct
    17761 ttcgataacc cctgaccagc gcctgcaaat gctgatcgtc ggtttctgtt tcggcgcttt
    17821 ccttgaagga gccgcaggct ttggcgcacc ggtagcaatt accgccgcat tgctggtcgg
    17881 cctgggcttt aaaccgctgt acgccgccgg gctgtgcctg attgttaaca ccgcgccagt
    17941 ggcatttggt gcgatgggta ttccgattct ggtcgccggg caggtaacag gcatcgacag
    18001 ctttgagatt ggtcagatgg tggggcgtca gctgccgttt atgaccatta tcgtgctgtt
    18061 ctggatcatg gcgattatgg acggctggcg cggtatcaaa gaaacctggc ctgcggtcgt
    18121 ggtggcgggt ggttcgtttg ctatcgcaca gtacctcagt tccaacttca ttgggccgga
    18181 actgccagac attatctctt cgctggtatc gctgctctgt ctgacactgt tcctcaaacg
    18241 ctggcaacca gttcgcgtat tccgcttcgg tgacttaggg gcgtcacagg ttgatatgac
    18301 gctggctcac acaggttaca cggcaggtca ggtgctgcgc gcctggacac cgttcctgtt
    18361 cctgaccgcc accgtaacgc tgtggagtat cccgccgttt aaagccctgt tcgcttcagg
    18421 cggtgcgctg tatgagtggg tgatcaacat tccggtgccg tacctcgata aactggttgc
    18481 ccgtatgccg ccagtggtca gcgaagcgac agcgtatgcc gccgtgttta agttcgactg
    18541 gttctctgcc accggtaccg ccattctgtt tgctgccctg ctctcgattg tctggctgaa
    18601 gatgaaacca tctgacgcta tcagcacctt cggcagcacg ctgaaagaat tggctctgcc
    18661 tatctactcc atcggtatgg tgctggcatt cgcctttatc tcgaactatt ccggttcgtc
    18721 atcaacactg gcgctggcac tggcgcacac cggtcatgca ttcaccttct tctcgccgtt
    18781 cctcggctgg cttggggtat tcctgaccgg gtcggatacg tcatctaatg ctttgtttgc
    18841 cgctttgcag gctaccgccg cacaacaaat tggcgtttct gacctgttgc tggttgcagc
    18901 caacaccacc ggtggtgtca ccggtaagat gatctccccg caatctatcg ctatcgcctg
    18961 tgcggcggta ggcctggtgg gcaaagagtc tgatttgttc cgctttactg tcaaacacag
    19021 cctgatcttc acctgtatgg tgggcgtgat caccacgctt caggcctatg tcttaacgtg
    19081 gatgattcct taatgattgt tttacccaga cgcctgtcag acgaggttgc cgatcgtgtg
    19141 cgggcgctga ttgatgaaaa aaacctggaa gcgggcatga agttgcccgc tgaacgtcaa
    19201 ctggcgatgc aactcggcgt gtcgagaaat tcactgcgcg aggcgctggc gaaactggta
    19261 agcgaaggtg tgctgctcag tcgacgtggc ggcgggacgt ttattcgctg gcgtcatgac
    19321 acatggtcgg agcaaaacat cgtccagccg ctgaaaacgc tgatggccga tgatccggat
    19381 tacagtttcg atattctgga agcccgctac gccattgaag ccagcaccgc atggcatgca
    19441 gcaatgcgtg ccacgcctgg cgaaaaagaa aagattcagc tttgttttga agcaacgctc
    19501 agcgaagacc cggatctcgc ctcacaagcg gacgttcgtt ttcatctggc gattgccgaa
    19561 gcctcacata acatcgtgct gctgcaaacc atgcgcggtt tcttcgatgt cctgcaatcc
    19621 tctgtgaagc atagccgcca gcggatgtac ctggtacccc cagttttttc acagctgacc
    19681 gaacaacatc aggcggtcat tgacgccatt tttgccggtg atgctgacgg ggcgcgtaaa
    19741 gcaatgatgg cgcaccttag ctttgttcac accaccatga aacgattcga tgaagatcag
    19801 gctcgccatg cacgcattac ccgcctgcct ggtgaccata atgagcattc gagggagaaa
    19861 aacgcatgat tatttccgca gccagcgatt atcgcgccgc agcgcaacgc attctgccgc
    19921 cgttcctgtt ccactatatg gatggggggg catattctga atacacgctg cgccgcaacg
    19981 tggaagattt gtcagaagtg gcgctgcgcc agcgtattct gaaaaacatg tctgacttaa
    20041 gcctggaaac gacgctgttt aatgagaaac tgtcgatgcc ggtggcgcta ggtccggtag
    20101 gtttgtgtgg catgtatgcg cgacgcggcg aagttcaggc tgccaaagcc gcggatgcgc
    20161 atggcattcc gtttactctc tcgacggttt ccgtttgccc gattgaagaa gtcgctccag
    20221 ctatcaaacg tccgatgtgg ttccagcttt atgtgctgcg cgatcgcggc tttatgcgta
    20281 acgcactgga gcgagcaaaa gcagcgggtt gttcgacact ggttttcacc gtggatatgc
    20341 caacgccggg agcgcgttat cgtgatgcgc attctgggat gagcggcccg aacgcggcaa
    20401 tgcgccgcta tttgcaggcg gtgacgcatc cgcaatgggc gtgggatgtg ggcctgaacg
    20461 gtcgtccgca tgatttaggt aatatctcgg cttacctcgg caaaccgacc ggactggaag
    20521 attacatcgg ctggttgggg aataacttcg atccgtccat ctcatggaaa gaccttgagt
    20581 ggatccgcga tttctgggat ggcccgatgg tgatcaaagg gatcctcgat ccggaagatg
    20641 cgcgcgatgc agtacgtttt ggtgctgatg gaattgtggt ttctaaccac ggtggccgcc
    20701 agctggacgg tgtgctgtct tctgcccgtg cgctgcctgc tattgcagat gccgtgaaag
    20761 gtgatatcgc cattctggca gatagcggga ttcgtaacgg gctggacgtt gtgcgtatga
    20821 tcgcgctcgg tgctgatacc gtactgctgg gccgcgcttt cctgtatgcg ctggcaacag
    20881 cgggccaggc gggtgtagct aatctgctaa atctgatcga aaaagagatg aaagtggcga
    20941 tgacgctgac tggcgcgaaa tcgattagcg aaattacgca agattcgctg gtgcaggtgc
    21001 tgggtaaaga gttgccggcg gcattagctc cgatggcgaa agggaatgca gcttaagggt
    21061 tagacgaata tctgctatcc tgcccccgca ctaagggggc agtatgctaa acatcgtact
    21121 ttacgaacca gaaattccgc caaatactgg caacatcatc cgtctttgcg ctaataccgg
    21181 ctttcgtctg catatcatcg aaccgatggg atttgcctgg gacgataagc gcctgcgccg
    21241 cgcggggctg gactatcacg agtttaccgc cgttacgcgt catcatgact atcgcgcgtt
    21301 tctcgaagca gaaaatcccc agcgcctgtt tgccctcacc acgaaaggta cgcctgctca
    21361 cagcgccgta agctatcagg atggcgatta tctgatgttc ggcccggaaa cacgcggcct
    21421 gccagcgagc attcttgatg ccctgcccgc tgaacaaaaa attcgtattc caatggtgcc
    21481 ggacagtcgc agcatgaacc tgtccaatgc ggtgtcggtc gtggtgtatg aagcctggcg
    21541 gcagttgggg tatcctggag cggtgttgag agattagtta ctgtaggccg gatagatgat
    21601 tacatcgcat ccggcacgag cacaggacat cagatcccat caccatactc aaacgtatgg
    21661 ttaatgccgt tgaaatgctg atccatatcc attgacggtt tatcgctatc cggcttgcca
    21721 acaatgcgtg ccggaacgcc agcggcagta gtgtgcggcg gcaccggttg cagcaccacg
    21781 gaacctgcgc caatcttcgc gccgcgccca acttcaatat tgccgagaat tttcgcgccc
    21841 gcgccaatca tcacaccttc acgaattttc gggtgacggt cgccaccaga tttacccgta
    21901 ccgccaagcg tcacagattg cagaatcgat acgtcgtttt caatcaccgc cgtttcaccc
    21961 acaacgatgc ccgtcgcgtg gtcgagcatg ataccgcgac caatttttgc cgccgggtga
    22021 atatcaacct ggaacgttac agagacctgg ttttgtagaa aaatagccag cgcgcgacgg
    22081 ccctgattcc acaaccagtg accgatgcga tacgcctgca aagcatgaaa acccttcaga
    22141 tatagcaacg gggttgagta tttatccacc gccgggtcac gggtacgcac cgcctgaata
    22201 tcacaggccg cagaggcgat catttccggg tcggcggcgt aggcttcttc caccacttca
    22261 cggatagcaa tagccggcat aatgggcgat gacagcttgt tcgccagcat gtagctcagt
    22321 gcactgccaa ggttttcgtg cttgagtagc gtcgcgtggt aaaaactggc cagcattggc
    22381 tcacagtccg ccagcgttct ggcttcagct ttaatattgt tccagacaat ttccagttct
    22441 tcacacgaca ttgcttactc cacacgataa gataatgacc gacccgttct gcgcgggccg
    22501 ggtcatagtg gtaacaaagg ttccctgggt ttagtggctg ctgcgctcgt ccttgcgtgc
    22561 acgacctagt aaagtcaatg ctgcctcgcg cgcgtttttt ccgcaatata atacttgata
    22621 aatttcctcg gttattggca tttcaacgcc gaagcgatgc gccagttcgc ggacttcttt
    22681 cgtattgcgg tagccttcaa ccacctgacc aatcttctcc tgcgcgcttt gtacatccat
    22741 gccctgaccg agcatcatgc caaaacggcg gttacgcgac tggttgtcgg tacaggtaag
    22801 caccagatcg cccagccccg ccatgcccat aaaggtggca ggatcggcac ccagcgccgc
    22861 accaagacgc gacatttcag ccagcccacg ggtgatcagt gccgtacgcg cattcgcacc
    22921 aaaaccgata ccgtcggaca tccccgcgcc aatggcaatg acgtttttca ccgcgccgcc
    22981 aagctgcaca ccgatgaaat ccgggttgct gtaaacgcgg aaacttttgc cacagtgcaa
    23041 caattgctgg agatcatcgg caaaggtctg gtcggtagag gccagcgaaa tggccgttgg
    23101 tagacctgcc gccagttctt tcgcaaacgt tgggccagag ataaccgcca acggaatttg
    23161 atcgcctaag gcctcacgcg ccacgtcctg taacagacgt ccggtttctg cttccagccc
    23221 tttggtcgcc cacaccagac gcgcatcagg acgcatcagc ggtttaatct gacgcagcac
    23281 ttcaccaaag acatggctgg gtacgacgac gagaatatta cggctggctg ccagcgcagt
    23341 ggcgagatcg ctttcaagat ggagcgtatc gggaaaaggc acatcgggga gaaacgcggc
    23401 gttacagcgc tcgcgttcaa gcgttgcgat atgttcaggg tcatggcccc agaggacaac
    23461 ctcgtggcca tttcttgcca gggtaatggc aagagcggtg ccgtacgagc cggcaccgat
    23521 cacagtcatt gaagcattac gttggttcat caggcatcct gatgttcttc agtaccttcg
    23581 ccagcctgct gctgcaaata gttcatgaac agcgcatcga agttaaccgg cgcaaggttc
    23641 agttgcggga atgtaccgcg ggataccatg ctggtgatgc actcacgagc atacgggaac
    23701 agaatgttcg ggcagtatgc gcccaggcaa tgtgccatct gggtgccttc gatacccgcg
    23761 atggagaaaa taccgccctg ctgaacttca cacaggaacg cggtttcttc gcccagggag
    23821 gccgttacgg taacacgcag taccacttcg taaacgtcat ctgccagttg agtagatgcc
    23881 gtatccagat caagtttaac ttctggttgc caatctttct ggaaaacgtg cggcgcgttc
    23941 ggcgcttcga aagaaatatc cttggtataa atacgttgga tctggaaagt catttcggtg
    24001 ttgttttgtt ctgacatgtg tagaaaaccc ttaagtgttg tccttaaata ctgcgcaatg
    24061 taatgccaac gttcacacgt tatttcagca ggggatccag tccaccacgt gcatccaatg
    24121 catacaagtc atcacaaccg ccaatgtgct gtgcgtcaat aaaaatctgg ggaaccgtgg
    24181 tgcgaccgct gcgtttgatc atctcttcac gcttagcggc gttgccatcg atcggcaact
    24241 cctgaaaact cacgcccttg ctgctcagca gtgcttttgc acgatggcaa tacgggcagg
    24301 tttctttggt atagatttca acattggcca taaattagct cctgtttttt accctgaaat
    24361 ttcatgttgc agggcggcag caaaatggag tgtccccaga agcttatatc aataagtcgc
    24421 tggaggccta attgcagcta acggcctgca tcatgaaaga cgacagataa attatttgcc
    24481 gcgcaccaaa ggcaagtttt cgccagccca gccagcgacg ccttctttca gtacgaatac
    24541 ttgcgcaaaa ccagctttcg tcagcgcgtt tgcaggctcc tggcactgca tgccagaacc
    24601 atctaccacg ataaccggtt tgtctttgtg cttctcaagc tcaccaacat tgttggcttt
    24661 gatttcgctc ggcaacaggt tgatagaacc tgcgatatgg cctttacgga agtcatcacg
    24721 ctgacgtaaa tccacaacca cagcgtcttc tttgttgatc agacgcgtag cttcaccacg
    24781 agtaatcacc ttcactttcg aggtcaggct cttaaacgta gtcacaagaa ccgccaccag
    24841 taacgcgatc caggcgatac tcagtatggg atgacggcca acaaattgca taatttcttg
    24901 catggggggt aacaactccc gacgtagtga ttaaaaaaac caggaaagga gtatacctgc
    24961 gcgttgcggc aaatacagcc agcgcgttaa ctggaatgca attttgcggg gcgctacgaa
    25021 aaaaaagcgt aaatctggca tctgcttgcc gcgagcggtc gtatattttg atcttcagag
    25081 gctattttat cgattcagct gtagtaaaat tacgcaaatt ttgactcttg agtatgaggt
    25141 tgtcgcaatg tcggtttcta aaaaacctat ggtactggtg attctggatg gctatggcta
    25201 tcgcgaagaa cagcaggata acgccatttt tagtgctaaa accccggtaa tggatgcact
    25261 gtgggccaat cgtccgcata ccctgatcga cgcttccggt ttggaagtcg gtctgcctga
    25321 ccgtcagatg ggcaactccg aagtaggcca cgttaacctg ggtgccggcc gcatcgtgta
    25381 tcaggacctg actcgtctgg acgttgaaat caaagatcga gctttctttg ctaatccggt
    25441 gttgactggt gcagtagata aagcgaaaaa cgcaggcaaa gcagtacaca ttatgggtct
    25501 gctctccgca ggtggtgtac acagccacga agatcacatc atggcgatgg tagaactggc
    25561 agctgaacgc ggcgcagaaa aaatctacct acacgcattc cttgacggtc gcgacactcc
    25621 gccgcgcagt gctgaatcct cgctgaaaaa attcgaagag aaatttaccg cgctgggtaa
    25681 aggccgcgta gcgtccatca ttggtcgtta ctacgcgatg gaccgcgata accgttggga
    25741 tcgcgttgaa aaagcttatg acctgctgac tctggcgcaa ggtgagttcc aggccgatac
    25801 cgccgttgcc ggtttgcagg ctgcttatgc tcgcgacgaa aacgacgagt tcgtgaaagc
    25861 gaccgttatt cgtgcagaag gtcagccaga tgcggcaatg gaagacggcg atgcactgat
    25921 tttcatgaac ttccgtgctg accgcgcgcg tgaaatcact cgtgcattcg taaatgccga
    25981 ttttgacggc ttcgcgcgta agaaagtggt taacgtcgat ttcgtgatgc tgaccgaata
    26041 cgctgccgac atcaaaaccg cagtggctta cccacccgct tccctggtta acaccttcgg
    26101 cgaatggatg gcgaaaaacg acaaaactca gctgcgtatt tccgaaaccg aaaaatatgc
    26161 ccacgttact ttcttcttca acggtggcgt agaagagtcg ttcaaaggcg aagatcgcat
    26221 tctgatcaac tcaccgaaag tggctaccta cgatctgcaa ccggaaatga gctccgcaga
    26281 gctgaccgaa aaactggttg cggcaatcaa gagcggcaaa tacgacacca ttatttgtaa
    26341 ctatccgaac ggcgacatgg taggtcacac cggagtgatg gaagcggcgg ttaaagcggt
    26401 tgaagcgctg gatcactgcg tggaagaagt cgcgaaagcg gttgagtccg tgggtggaca
    26461 actgctgatc accgctgacc acggtaacgc tgaacagatg cgcgatccgg caacgggtca
    26521 ggcacacaca gcacacacca acctgccagt tccgctgatt tacgttggtg ataagaacgt
    26581 gaaagcggtt gaaggcggca aactttctga catcgcgccg accatgttgt cgctgatggg
    26641 tatggaaatc ccgcaagaga tgactggtaa gccgctgttc atcgtggaat aatccctccc
    26701 catgagggga aaggcgatta ataccatgac acgggccgtg aaaccgcgca ggtttgcaat
    26761 caggcccatc atctacgcca gcgttctgag cgctggcgta ttgttgtgcg ccttttccgc
    26821 ccacgcggat gagcgtgacc aactcaaatc cattcaggcc gatatcgccg cgaaagagcg
    26881 cgcggtacgc caaaagcaac aacaacgcgc aagcctgctc gcacaattga aaaagcagga
    26941 agaagcgatc tctgaagcca ctcgcaaact gcgcgaaacg caaaatacgc tcaatcaact
    27001 gaataaacag attgatgaga tgaacgcgtc gattgccaaa ctggagcagc aaaaagccgc
    27061 ccaggagcgc agcctcgccg cgcaactgga tgccgcgttt cgtcagggtg aacataccgg
    27121 tattcagctg attctcagcg gtgaagaaag ccagcgtggg cagcggttac aggcttattt
    27181 cggctatctc aaccaggcgc gacaagaaac cattgctcag ttgaaacaaa cgcgtgaaga
    27241 agtcgctatg cagcgtgccg aactggaaga gaaacaaagc gagcaacaaa cgcttttata
    27301 tgagcagcgc gcccaacagg cgaagctgac ccaggcgttg agcgagcgta aaaagacgct
    27361 ggcagggctg gagtcttcca tccagcaagg tcagcaacag ttgagcgagc tgcgcgccaa
    27421 cgaatcccgc ctgcgtaaca gcattgcccg tgcggaagct gcggcgaaag cgcgtgctga
    27481 acgtgaagcg cgcgaagccc aggcggttcg cgaccgccag aaagaagcga cgcgcaaagg
    27541 caccacctac aagccgaccg aaagcgaaaa atcgctgatg tcccgtaccg gtggtctggg
    27601 ctcaccgcgc ggtcaggcat tctggccagt tcgcgggccg acgctgcatc gctatggcga
    27661 acagctacag ggtgaattac gctggaaagg gatggtgatt ggtgcctctg aaggtactga
    27721 agttaaagcg attgccgacg gtcgggtgat tctggctgac tggctgcaag gctacggtct
    27781 ggtggtggtg gttgagcatg gtaaaggcga catgagtctt tacggctata atcagagcgc
    27841 actggtgagc gttggttcgc aggttcgcgc gggccagcca attgcactgg tgggcagcag
    27901 tggcggtcag ggtcggcctt cactctattt cgaaattcgc cgccagggtc aggcggtcaa
    27961 tccacagccg tggttgggaa gataagtttt gtttccattt cgtcgtaacg ttcttgcatt
    28021 tgccgctctg ttggcgctct cctcccccgt aattgctggc aaacttgcca tcgtcattga
    28081 tgattttggg tatcgcccgc acaacgaaaa ccaggtgctg gcgatgcctt ccgcgatctc
    28141 cgtcgctgta ttacccgatt caccgcacgc cagagagatg gcgaccaaag cgcataacag
    28201 cggtcacgaa gtgttgattc atctaccgat ggcgccactg agtaaacagc cgctggagaa
    28261 aaatacgcta cgcccggaga tgagcagcga cgaaattgag cgcattattc gtagtgcggt
    28321 caataacgtg ccctatgccg tggggatcaa caaccacatg ggcagcaaga tgacctctaa
    28381 cctgtttggt atgcagaaag tgatgcaggc gctggagcgt tacaatcttt acttcctcga
    28441 cagcgtaacc atcggtaata cccaggcgat gcgcgccgcg caaggcactg gcgtgaaggt
    28501 gatcaaacgg aaggtgttcc tcgacgattc gcagaacgaa gcggacatcc gtgtgcaatt
    28561 taatcgcgca attgatctgg cgcgtcgcaa cggttcaacc attgccattg ggcatcctca
    28621 cccttcaacg gtacgcgtgc tacaacagat ggtttataac ctgccgccag acattacgct
    28681 ggtgaaagcc agcagcttgc tgaatgaacc gcaggttgat acgtctacac cgccgaaaaa
    28741 cactgtgccc gacacaccgc gtaatccatt ccgtggcgtg aagctgtgca aaccgaagaa
    28801 gccgctggaa cctgtttatg ctaatcgctt ctttgaagta ttaagcgaaa gcatcagcca
    28861 gagcacgctg atcgtttact tccagcatca gtggcaaggc tggggcaaac agcccgaagc
    28921 ggcaaaactt aacgctagcg caaattaaag gcgctacgcg taatgcgatg gtgcgaccgc
    28981 gttttgtcgc gccattgcca taagcgg
//
LOCUS       DAZFFE010000054        28789 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFFE010000054 DAZFFE010000000
VERSION     DAZFFE010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.054"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..698
                     /gene="bcsQ"
                     /locus_tag="ACHJ69_002555"
                     /pseudo
     CDS             1..698
                     /gene="bcsQ"
                     /locus_tag="ACHJ69_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
     gene            695..3313
                     /gene="bcsA"
                     /locus_tag="ACHJ69_002556"
     CDS             695..3313
                     /gene="bcsA"
                     /locus_tag="ACHJ69_002556"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO5015516.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLFHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIIL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPAQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            3324..5660
                     /gene="bcsB"
                     /locus_tag="ACHJ69_002557"
     CDS             3324..5660
                     /gene="bcsB"
                     /locus_tag="ACHJ69_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO5015517.1"
                     /translation="MKRKIFWICAVALGMSAFPSFMTQATPATQPLINAEPAVAAQAE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVELQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIVGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRLNPDNE"
     gene            5667..6773
                     /gene="bcsZ"
                     /locus_tag="ACHJ69_002558"
                     /pseudo
     CDS             5667..6773
                     /gene="bcsZ"
                     /locus_tag="ACHJ69_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
     gene            6755..10228
                     /gene="bcsC"
                     /locus_tag="ACHJ69_002559"
     CDS             6755..10228
                     /gene="bcsC"
                     /locus_tag="ACHJ69_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO5015518.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVIAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVVNLEKALALDP
                     HNSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAYIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRGQWNSNIQE
                     LVNRLQNDQVLETANRLRENGKEAEAEAMLRQQPPSSRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPAIDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEALAGDPTQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDTADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            10310..12298
                     /gene="hmsP"
                     /locus_tag="ACHJ69_002560"
     CDS             10310..12298
                     /gene="hmsP"
                     /locus_tag="ACHJ69_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO5015519.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPPQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12481..13767
                     /gene="dctA"
                     /locus_tag="ACHJ69_002561"
     CDS             12481..13767
                     /gene="dctA"
                     /locus_tag="ACHJ69_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO5015520.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            13987..15483
                     /locus_tag="ACHJ69_002562"
     CDS             13987..15483
                     /locus_tag="ACHJ69_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO5015521.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPVQ"
     gene            complement(15579..16508)
                     /gene="kdgK"
                     /locus_tag="ACHJ69_002563"
     CDS             complement(15579..16508)
                     /gene="kdgK"
                     /locus_tag="ACHJ69_002563"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO5015522.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWRGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16739..17506
                     /gene="pdeH"
                     /locus_tag="ACHJ69_002564"
     CDS             16739..17506
                     /gene="pdeH"
                     /locus_tag="ACHJ69_002564"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO5015523.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVMHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIEDLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            17576..19636
                     /locus_tag="ACHJ69_002565"
     CDS             17576..19636
                     /locus_tag="ACHJ69_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO5015524.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FQLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLIYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGIAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINITGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMRK"
     gene            complement(19677..20999)
                     /locus_tag="ACHJ69_002566"
     CDS             complement(19677..20999)
                     /locus_tag="ACHJ69_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5015525.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFAVMVPLAGLLADAFGRRKSMVVITTLIILFALF
                     AFNPLLGSGNPALVFVFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21392..22405)
                     /gene="yhjD"
                     /locus_tag="ACHJ69_002567"
     CDS             complement(21392..22405)
                     /gene="yhjD"
                     /locus_tag="ACHJ69_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO5015526.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22454..23353)
                     /locus_tag="ACHJ69_002568"
     CDS             complement(22454..23353)
                     /locus_tag="ACHJ69_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5015527.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARSLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            23873..24475
                     /locus_tag="ACHJ69_002569"
     CDS             23873..24475
                     /locus_tag="ACHJ69_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO5015528.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQHRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTAVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(24526..26175)
                     /locus_tag="ACHJ69_002570"
     CDS             complement(24526..26175)
                     /locus_tag="ACHJ69_002570"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO5015529.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARNWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            26580..27977
                     /gene="ccp"
                     /locus_tag="ACHJ69_002571"
     CDS             26580..27977
                     /gene="ccp"
                     /locus_tag="ACHJ69_002571"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO5015530.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LANKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDRWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            28188..>28789
                     /locus_tag="ACHJ69_002572"
     CDS             28188..>28789
                     /locus_tag="ACHJ69_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO5015531.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDE"
BASE COUNT         6838 a         7246 c         7921 g         6784 t
ORIGIN      
        1 ccgccgcatt agcctggtca ttacaaatgt tgggagaaaa tgtcctggtg gtcgatgcct
       61 gcccggacaa cttgttgcgc ctgtcattta acgttgattt tacccaccgt cagggttggg
      121 ccagagctat gctggatggc caggactggc gtgacgccgg gttgcgctat acctcgtagc
      181 tcgatttgct gccttttggt cagttatcca ttgaagaaca agaaaatcca cagcactggc
      241 aaacccggct gagcgatatt tgcaccggct tacagcaact aaaagccagc gggcgttacc
      301 agtggatttt aattgactta ccgcgtgatg cctcgcagat aacccaccag ctgctgagtt
      361 tgtgcgatca ctcgctggca atcgtcaatg tggatgccaa ctgccatatc cgactgcatc
      421 agcaagcgct gccggatggt gcacatattt tgattaatga cttccgtatt ggcagccagg
      481 ttcaggacga tatttaccag ctttggttgc aaagccagcg ccgattgctg ccgatgctca
      541 ttcatcgtga tgaagcgatg gctgaatgtc tggcagctaa gcaaccagtc ggtgaatatc
      601 gcagtgatgc gctggcggct gaagagatat tgacgctggc gaactggtgc ctgttgaatt
      661 attccgggct gaaaacgcca gtcgggagtg catcatgagt atcctgaccc ggtggttgct
      721 tatcccgccg gtcaacgcac ggcttattgg gcgttatcgc gattatcgtc gtcacggtgc
      781 gtcggctttc agcgcgacgc tcggctgttt ctggatgatc ctggcctgga tttttattcc
      841 gctggagcac ccgcgctggc agcgtattcg cgcagaacat aaaaacctgt atccgcatat
      901 caacgcctcg cgtccgcgtc cgctggaccc ggtccgttat cttattcaaa catgctggtt
      961 actgatcggt gcatcgcgca aagaaacgcc gaaaccgcgc aggcgggcat tttcaggtct
     1021 acaaaatatt cgtggacgtt accatcaatg gatgaacgag ctgcctgagc gcgttagcca
     1081 taaaacacag catcttgatg agaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg
     1141 gttgatcctt ggtatcatcg tcaccttctc gctgattctg gcgttaatct gcgttactca
     1201 gccgtttaac ccgctagcgc agtttatctt cctgatgctg ctgtgggggg tagcgctgat
     1261 cgtacggcgg atgccggggc gcttctcggc gctgatgctg attgtgctgt cgctgaccgt
     1321 ttcttgccgt tatatctggt ggcgttacac ctcgacgtta aactgggacg atccggtcag
     1381 cctggtgtgc gggcttattc tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct
     1441 cggctatttc caggtcgtat ggcctctgaa tcgtcagccg gtgccattgc cgaaagatat
     1501 gtcgctgtgg ccgtcggtgg atatctttgt cccgacttac aacgaagatc tcaacgtggt
     1561 gaaaaatacc atttacgctt cgctgggtat cgactggccg aaagacaagc tgaacatctg
     1621 gatcctagat gacggcggca gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa
     1681 atatatcgcc cgtaccactc atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa
     1741 atatgccaaa ggcgagttcg tgtcgatatt cgactgcgac cacgtaccaa cgcgatcgtt
     1801 cctgcaaatg accatgggct ggttcctgaa agagaaacag ctggcgatga tgcagacgcc
     1861 gcaccacttc ttctcgccgg acccgtttga acgcaacctg gggcgttttc gtaaaacgcc
     1921 gaacgaaggc acgctgttct atggtctggt gcaggacggc aatgatatgt gggatgccac
     1981 tttcttctgc ggctcatgtg cggtgattcg ccgtaagccg ctggatgaga ttggcggtat
     2041 tgctgtcgaa acggtaaccg aagatgcgca cacttctctg cgtctgcacc gtcgtggcta
     2101 cacctccgcg tatatgcgta ttccgcaggc agcggggctg gcgaccgaaa gtctctcggc
     2161 gcatatcggt cagcgtattc gctgggcgcg cgggatggtg caaatcttcc gtctcgataa
     2221 cccgctcacc ggtaaagggc tgaagtttgc tcagcggctg tgctatgtca acgccatgtt
     2281 ccacttcttg tcgggcattc cacggctgat cttcctgact gcgccgctgg cgttcctgct
     2341 gtttcatgcc tacatcatct acgcgccagc gttgatgatc gccctgtttg tgctaccgca
     2401 tatgatccat gccagcctga ctaactcgaa gatccagggc aaatatcgcc actctttctg
     2461 gagtgaaatc tacgaaacgg tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct
     2521 gattaacccg cacaaaggca aatttaacgt caccgccaaa ggtggactgg tggaagaaga
     2581 gtacgtcgac tgggtgatct cgcggcccta catcttcctt gttctgctca acctggtggg
     2641 cgtagcggtt gggatctggc gctacttcta tggtccgcca actgagatgc tcaccgtggt
     2701 cgtcagtatg gtgtgggtat tctacaacct gattattctc ggcggcgcag tcgcagtatc
     2761 ggtagaaagc aaacaggtac gccgatcgca ccgcgtggag atgacgatgc ccgcagcaat
     2821 tgcccgcgaa gatggtcacc tcttctcgtg taccgttcag gatttctccg acggtggttt
     2881 ggggatcaag atcaacggtc aggcgcagat tctggaaggg cagaaagtga atctgttgct
     2941 taaacgcggt cagcaggaat acgtcttccc ggctcaggtg gcgcgcgtga tgggtaatga
     3001 agttgggctg aaattaatgc cgctcactac ccagcaacat atcgattttg tgcagtgtac
     3061 gtttgcccgt gcggatacat gggcgctctg gcaggacagc tacccggaag ataagccgct
     3121 ggaaagtctg ctggatattc tgaagctcgg cttccgtggc taccgccatc tggcggaatt
     3181 tgcgccttct tcggtgaagg gcatattccg tgtgctgact tctctggttt cctgggttgt
     3241 atcgtttatt ccgcgccgcc cggagcggag cgaaacggca caaccatcgg atcaggcttt
     3301 ggctcaacaa tgatgataac gcgatgaaaa gaaaaatatt ctggatttgt gcagtggctc
     3361 tggggatgag tgcgttcccc tctttcatga cgcaggcgac gccagcaacg caaccactga
     3421 tcaatgctga gccagctgta gccgcccagg cggagcaaaa tccgcaggtg gggcaagtga
     3481 tgccgggcgt gcagggcgca gatgcgccag tcgtggcgca gaacggtcct tcccgtgatg
     3541 tgaagctgac ctttgcgcaa attgcgccgc cgccgggcag catggtgcta cgtggcatta
     3601 acccaaacgg cagcattgag tttggtatgc gcagcgatga agtggtgacg aaggcgatgc
     3661 tcaacctcga atacacgcca tcgccatcat tactgcctgt ccagtcgcag ttaaaggttt
     3721 atctcaatga tgaactgatg ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa
     3781 cgctggcaca aatgcccatt aacccgctgt ttattactga tttcaaccgt gtgcggctgg
     3841 agtttgtcgg ccattatcag gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg
     3901 atgttgggcg gagcagtgga ctggatctga cctatcagac cctgaatgtg aagaatgacc
     3961 tgtcacactt cccggtgcca ttctttgacc cgcgtgataa ccgcaccaac accttgccga
     4021 tggtctttgc gggtgcgccg gatgttgagc tgcaacaagc atctgccatt gtcgcctcgt
     4081 ggtttggttc gcgttctggc tggcgtgggc agaacttccc ggtgctctat aaccaactgc
     4141 cggatcgcaa tgccattgtc tttgccacca atgacaaacg cccggacttc ctgcgcgatc
     4201 atccggcggt aaaagccccg gtgattgaga tgattaacca tccgcagaat ccttacgtca
     4261 aactgctggt ggtgtttggt cgtgacgaca aagacttgtt gcaggcagcg aaaggtatcg
     4321 ctcagggtaa cattctgttc cgtggtgaaa gtgtggtagt gaatgaagtg aaaccgctgc
     4381 taccacgtaa gccgtacgat gcgccgaact gggtgcgtac cgatcgtccg gtcacctttg
     4441 gtgaactgaa aacctatgaa gaacaattac aatccagcgg tcttgagcca gcagcgatta
     4501 acgtttcgct aaacctgccg ccggatctct acctgatgcg cagtaccggc attgatatgg
     4561 atattaatta ccgctacacc atgccgccgg tgaaagacag ttcgcggatg gatatcagcc
     4621 tgaataacca gttcctgcaa tccttcaacc tgagcagcaa acaggaggcg aaccgcctgc
     4681 tgctgcggat tccggtatta caaggtttgc tggatggcaa aacggatgtc tctattccgg
     4741 cgctgaaact gggcgcgacc aaccagctgc ggttcgactt tgagtatatg aacccgatgc
     4801 cgggcggttc ggtggataac tgcattacct tccagccggt gcagaatcat gtggtgattg
     4861 gtgacgactc caccatcgac ttctcgaagt attaccactt catcccgatg ccggatctac
     4921 gcgcctttgc taacgcgggc ttcccgttca gccggatggc ggatttgtcg caaaccatca
     4981 ccgtgatgcc gaaaacgcca aacgaagcac agatggaaac cttgctgaat accgttggct
     5041 ttatcggtgc gcagacgggc ttcccggcga ttaacctgac ggtgaccgat gatggcagca
     5101 ccattcaggg caaagatgcc gacatcatga tcgtcggtgg tatcccggac aaactgaaag
     5161 acgataagca gatcgacctg ttggtgcagg ctaccgaaag ctgggtgaaa acgccgatgc
     5221 gccagacccc gttccccggt attgtgccgg acgagagcga tcgcgcggca gaaacccagt
     5281 caacgctgac ctcttccggt gcgatggcgg cggtgattgg tttccagtcg ccgtataacg
     5341 accagcgcag cgtgattgcg ctgctggcag atagcccacg cggttatgaa atgcttaacg
     5401 atgcggtgaa cgatagcggc aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg
     5461 agtccggtat caacagccta cgtgttggcg atgtttatta cgtaggtcat ctgccgtggt
     5521 tcgagcgctt gtggtatgcg ctggcaaacc atccgattct gctggcggtg ctggcggcta
     5581 tcagtgtgat attgctggcg tgggtactgt ggcgtctgct gcgtattatt agtcgtcgtc
     5641 ttaacccgga taacgagtaa ttgaagatga atgtgttgcg tagtggactc gtgacgatgc
     5701 tgctgctggc tgcctttagt gttcaggcag cctgtaccta gcctgcctgg gagcagttta
     5761 aaaaggatta catcagtcag gaagggcgcg tcatcgaccc cagcgacgcg cgcaaaatca
     5821 ccacctccga agggcaaagt tacggcatgt tctttgccct ggcggctaac gaccgtgcag
     5881 ctttcgataa tcttctcgac tggacgcaga acaatctcgc tcagggttct ttaaaagaac
     5941 atttgcccgc ctggctgtgg ggcaagaaag agaacagtaa gtgggaagtg ctggacagca
     6001 attcggcctc cgatggtgat gtctggatgg cctggtcgtt gctggaggcg gggcgtttgt
     6061 ggaaagagca gcgttatacc gacatcggca gcgcattgct aaaacgtatc gcgcgggagg
     6121 aagtggtgac ggtgcctggg ctgggctcca tgttgttacc gggcaaagtg ggttttgctg
     6181 aggataacag ctggcgtttt aaccccagct acctgccgcc gacgctggcg cagtatttca
     6241 cccgctttgg cgcgccgtgg accacgctgc gcgaaaccaa tcaacgttta ttgctggaaa
     6301 ccgccccgaa aggcttttcg ccagactggg tgcgctatga gaaagacaaa ggctggcagc
     6361 taaaagccga aaaaacattg atcagcagct acgacgctat ccgcgtttac atgtgggtag
     6421 gcatgatgcc tgacagcgat ccgcaaaaag cgcggatgct caaccggttt aaaccgatgg
     6481 cgacattcac tgagaaaaac ggttatccgc cggaaaaagt ggatgtggct acggggaaag
     6541 cgcagggtaa aggaccggtc ggtttttctg ccgccatgct gcccttttta caaaaccgcg
     6601 atgcgcaggc cgttcagcgc cagcgcgtgg ccgataactt tcccggcagc gatgcctatt
     6661 acaactatgt gctgaccctg tttggacaag gctgggatca acaccgtttc cgcttctcga
     6721 caaaaggtga gttattacct gactggggcc aggaatgcgc aaattcacac taaacatatt
     6781 cacgctttcc ctcggtctgg ccgtcatgcc gatggtcgag gcagcaccaa ccgctcagca
     6841 acagttgctg gagcaagttc ggttaggcga agcgacccat cgtgaagacc tggtgcaaca
     6901 gtcgttgtat cggctggaac ttattgatcc gaataacccg gatgtgattg ctgcccgttt
     6961 tcgttctctg ttacgtcagg gcgatattga tggcgcacaa aaacagctcg atcggctgtc
     7021 gcagttagcg ccgagttcaa atgcgtataa atcgtcgcgg accacaatgt tactgtctac
     7081 gccggatggt cgtcaggcac tgcaacaggc tcgattgcag gcgactaccg gtcatgcgga
     7141 agaggccgtg gcgagttaca acaagctgtt caacggtgcg ccgccggaag gtgacattgc
     7201 tgtcgagtac tggagtacgg tggcgaaaat tccggctcgc cgtggtgaag cgattaatca
     7261 gctaaaacgc attaatgcgg atacgccggg caatacgggc ctgcaaaaca atctggcgct
     7321 attactgttt agtagcgatc gccgtgacga aggttttgcc gtcctggaac agatggcgaa
     7381 atcgaacgcc gggcgcgaag gggcctctaa aatctggtac gggcagatta aagacatgcc
     7441 cgtcagcgat gccagcgttc aggcactgaa aaaatatctc tcgatcttta gcgatggcga
     7501 tagcgtggct gctgcacaat cgcaactggc agaacagcaa aaacagctgg ccgaccctgc
     7561 tttccgcgct cgtgcgcaag gtttagcggc ggtggactct ggcatggcgg gtaaagccat
     7621 tcccgaacta caacaggcgg tgcgggcgaa cccgaaagac agtgaggctc tgggggcgct
     7681 gggccaggcg tattcccaga aaggcgatcg cgccaatgca gtggtgaatc tggaaaaagc
     7741 cctcgcgctg gatccgcaca acagcaataa cgacaaatgg aacagcctgc tgaaagtaaa
     7801 ccgctactgg ctggcgatcc agcagggcga tgccgcgctg aaagccaata atcctgaccg
     7861 ggcagaacgg ctgttccagc aggcacgtaa tgtcgataac accgacagct atgcagtgct
     7921 tgggctgggc gatgtggcga tggcgcgcaa agattatccc gccgccgaac gctattacca
     7981 gcagaccttg cgtatggaca gcggcaacac taacgccgtg cgcgggctgg cgaatattta
     8041 ccgccagcag tcgccagaaa aagccgaagc gtatatcgcc tcgctctctg ccagccagcg
     8101 gcgcagcatt gatgatatcg aacgcagcct gcaaaacgac cgtctggcac agcaggcaga
     8161 ggcactggaa aaccagggga aatgggcgca ggcggcagca cttcagcggc aacgactggc
     8221 gctggacccc ggcagtgtat ggattactta ccgactttcg caggatctct ggcaagccgg
     8281 acaacgcagc caggccgata cgttaatgcg caatctggcg cagcagaagc cgaacgaccc
     8341 ggagcaggtt tacgcttacg ggctgtacct ctccggtcac gaccaggaca gagcggcgct
     8401 ggcgcatatc aacagcctgc cgcgcgggca gtggaacagc aatattcagg agctggttaa
     8461 tcgactgcaa aacgatcagg tgctggaaac cgctaatcgc ctgcgagaaa acggtaaaga
     8521 ggcagaagcg gaagcgatgc tgcgccagca accgccttcc tcgcgtattg acctcacgct
     8581 ggctgactgg gcgcagcagc gacgtgatta caccgccgcc cgcgctgcat atcagaatgt
     8641 cctgacgcgg gagccaacta acgccgatgc cattcttggt ctgacggaag tggatattgc
     8701 tgccggtgac aaagcggcgg cacgtagcca gctggcgaaa ctgcctgcca ttgataacgc
     8761 ctcgctgaac acacagcggc gcgtggcgct ggcacaggcg cagcttggcg ataccgcagc
     8821 ggcgcagcag acatttaata agttgatccc gcaggcaaaa tctcagccac cgtcgatgga
     8881 aagcgcgatg gtactgcgtg atggtgcgaa gtttgaagcg ctggcgggcg atccaacgca
     8941 ggcgctggaa acctacaaag acgctatggt tgcatccggt gtgaccacga cgcgtccgca
     9001 ggataacgac acctttaccc ggctgacccg taacgacgag aaagatgact ggctgaaacg
     9061 cggcgtgcgc agcgatacgg cggacctcta tcgccagcag gatcttaacg tcactctcga
     9121 gcacgattac tggggttcga gcggtaccgg tggttactcc gatctgaaag cgcacaccac
     9181 tatgttgcag gtggacgcgc cctattctga cgggcggatg ttctttcgca gtgatttcgt
     9241 caatatgaac gtcggcagtt tctccactaa tgccgatggc aaatgggatg acaactgggg
     9301 cacctgtaca ttacaggact gtagcggcaa ccgcagccag tcggattccg gtgccagcgt
     9361 ggcggtcggc tggcgaaatg acgtctggag ctgggatatc ggtaccacgc cgatgggctt
     9421 caacgtggtg gatgtggtcg gcggcatcag ttacagcgat gatatcgggc cgctgggtta
     9481 caccgttaac gcccaccgtc ggcctatctc cagttctttg ctggcctttg gtgggcaaaa
     9541 agactctccg agcaataccg ggaaaaaatg gggcggcgtg cgtgccgacg gcgtggggct
     9601 aagtctgagc tacgataaag gtgaagcaaa cggcgtctgg gcatcgctta gcggtgatca
     9661 gttaaccggt aaaaatgtcg aagataactg gcgcgtgcgc tggatgacgg gctattacta
     9721 taaggtcatc aaccagaaca atcgccgcgt cacaatcggc ctgaacaaca tgatctggca
     9781 ttatgacaaa gacctgagtg gctactcact cggtcagggc ggttactaca gtccgcagga
     9841 atacctgtcg tttgccatac cggtgatgtg gcgggagcgc acggaaaact ggtcgtggga
     9901 gctgggggcg tctggctcgt ggtcgcattc acgcaccaaa accatgccgc gttatccgct
     9961 gatgaatctg atcccgaccg actggcagga agaagctgcg cggcaatcca acgatggcgg
    10021 cagcagtcag ggctttggct acacggcgcg ggcattactt gaacgacgtg ttacttccaa
    10081 ctggtttgtc ggcacggcaa ttgatatcca gcaggcaaaa gattacgccc ccagccattt
    10141 cctgctctac gtacgttatt ccgccgccgg atggcagggt gacatggatt taccgccgca
    10201 gccgctgata ccttacgccg actggtaagt tttcagatag cgcctctctt aatgccgctg
    10261 cgatcgggta tactcgggcg gcaatctggg atttccgggg ggagacaatt tgcgcgtaag
    10321 tcgctcgtta acaatcaagc agatggcaat ggtggcagcc gttgtcctgg tgttcgtttt
    10381 tattttttgc accgttttgc tgttccatct ggtccagcag aatcgctata acacggctac
    10441 gcaactggaa agcattgctc gctctgtccg cgaaccctta tcttctgcca ttttgaaagg
    10501 cgatattccc gaagcggaag ctattcttgc cagcattaaa ccggccggcg tggtcagccg
    10561 tgccgatgta gtgctgccca accagttcca ggcgctgcgt aaaagtttta ttccagagcg
    10621 tccggtgccg gtaatggtta ctcgcctgtt tgagctaccg gtgcaaatct cgctgggcgt
    10681 ttactcgctg gaacgtccgg caaacccgca gccaattgcc tatctggtgt tacaggcgga
    10741 ttccttccgc atgtataagt tcgtgatgag caccctctca acgttagtga ccatttactt
    10801 acttttgtcg ttaatattga cggtggcgat tagctggtgc attaaccgcc tgattttgca
    10861 tccgttacgc aatattgctc gcgaacttaa cgccatccca ccccaggagc ttgttggtca
    10921 ccaactggca ttaccgcgtc tgcatcagga cgatgaaatc ggtatgttgg tgcgcagtta
    10981 caacctcaac cagcaattgc tgcagcgcca ttatgaagaa cagaacgaaa atgcgatgcg
    11041 cttcccggtg tcggatttgc cgaacaaagc cttgctgatg gagatgctgg agcaggtggt
    11101 ggcgcgtaaa caaactaccg cgctgatgat catcacctgt gaaaccctgc gtgatactgc
    11161 gggtgtgctg aaagaggcgc aacgagaaat tctgctgctg acgctggtgg aaaaactcaa
    11221 atcggtactg tcgccacgca tgatcctcgc gcagattagc ggttatgact ttgctgtcat
    11281 tgccaacggt gtacaggaac cgtggcacgc tatcacatta ggtcagcaag tgctcactat
    11341 catgagcgag cgcctgccga ttgaacgtat tcaactccgt ccgcactgta gcattggcgt
    11401 ggcgatgttc tacggcgatc tcaccgccga acagctttac agtcgcgcta tttctgcggc
    11461 atttaccgct cgccataaag gcaaaaatca gattcagttc tttgatccgc agcagatgga
    11521 agccgcccag aagcggttga cggaagagag cgatatcctt aatgcactgg aaaatcatca
    11581 gtttgcgatt tggttacagc cacaggtcga gatgaccagc ggtaaactgg tcagtgcgga
    11641 agtgttactg cgtatccagc aaccggatgg cagttgggac ctgcccgatg gcttaatcga
    11701 tcgcattgag tgctgtgggc tgatggttac cgtcggtcac tgggtgctgg aagagtcctg
    11761 tcgattgctt gctgcctggc aagagcgcgg cattatgctg cccttgtcgg taaacctctc
    11821 agcgctgcaa ctgatgcatc caaatatggt ggcggatatg ctggaactgt taacccgcta
    11881 tcgcattcag ccgggaacac tgattctgga agtgacagaa agccgacgta ttgacgatcc
    11941 tcatgctgcg gtggcaatcc tccgtccgct gcgcaatgcc ggagttcggg tggcgctgga
    12001 tgatttcggc atgggctacg cagggctgcg tcagctgcag catatgaaat cgttgccaat
    12061 cgacgtactg aaaatcgaca aaatgtttgt tgaaggtttg ccggaagata gcagcatgat
    12121 tgctgcaatt atcatgctgg cgcagagcct gaacctacaa atgattgccg aaggcgtgga
    12181 gactgaagca caacgcgact ggctggcaaa agcgggcgtt ggtattgccc agggcttcct
    12241 ttttgctcgc ccactcccta ttgaaatctt cgaagagagt tacctggaag aaaagtagct
    12301 accccaaact gattacaaaa ctttaaaaag tgctggtttg tgcgagccag ctcaaacttt
    12361 ttaacctttt tgtttcaatt atgatccagg tacatttctg tgatgttgtc tgggtgttat
    12421 tttaaggccg caggtacccc ataaccttac aagacctgtg gttttactaa aggacaccct
    12481 atgaaaacct ctctgtttaa aagcctttac tttcaggtcc tgacagcgat agccattggt
    12541 attctccttg gccatttcta ccctgaaata ggcgagcaaa tgaaaccgct tggcgacggc
    12601 ttcgttaagc tcattaagat gatcatcgct cctgtcatct tttgtaccgt cgtaacgggc
    12661 attgcgggca tggaaagcat gaaggcggtc ggtcgtaccg gcgcagtcgc actgctttac
    12721 tttgaaattg tcagcaccat cgcactgatt attggtctta tcatcgttaa cgttgtgcag
    12781 cctggtgccg gaatgaacgt cgatccggca acgcttgatg cgaaagcggt agcggtttac
    12841 gccgatcagg cgaaagacca gggcattgtc gccttcatta tggatgtcat cccggcgagc
    12901 gtcattggcg catttgccag cggtaacatt ctgcaggtgc tgctgtttgc cgtactgttt
    12961 ggttttgcgc tccaccgtct gggcagcaaa ggccaactga tttttaacgt gatcgaaagt
    13021 ttctcgcagg tcattttcgg catcatcaat atgatcatgc gtctggcacc tattggtgca
    13081 ttcggggcaa tggcgtttac catcggtaaa tacggcgtcg gcacactggt gcaactgggg
    13141 cagctgatta tctgtttcta catcacctgt atcctgtttg tggtgctggt attgggttca
    13201 atcgctaaag cgacgggttt cagtatcttc aaatttatcc gttacatccg tgaagaactg
    13261 ctgattgtac tggggacttc atcttccgag tcggcgctgc cgcgtatgct cgacaagatg
    13321 gagaaactcg gctgccgcaa atcggtggtg gggctggtca tcccgacagg ctactcgttt
    13381 aaccttgatg gcacatcgat atacctgaca atggcggcgg tgtttatcgc ccaggccact
    13441 aacagtcaga tggatatcgt ccaccaaatt acgctattaa ttgtgttgtt gctctcttcc
    13501 aaaggggctg ccggggtaac gggcagtggc tttatcgtgc tggcggcgac gctctctgcg
    13561 gtgggccatt tgccggtagc gggtctggcg ctgatcctcg gtatcgaccg ctttatgtca
    13621 gaagctcgtg cgctgactaa cctggtcggt aacggcgtag cgaccattgt cgttgctaag
    13681 tgggtgaaag aactggacca caaaaaactg gacgatgtgc tgaataatcg tgcgccggat
    13741 ggcaaaacgc acgaattatc ctcttaatct caccacttat gcccgcactc ccttccccga
    13801 gtgcgggtgt ttgcgcataa ttgacccctg tgcctgaact taccacctgg gtgagttcag
    13861 gtttatttca cttcttccgt gacattttca tgttcttgcg gtctaacacg aagtgttttt
    13921 aacgtcattt agactgaccg acaacgggca gttttttatt accaggatag ttgatcaggg
    13981 gttcacatgc agggcacaaa aattcgactt ttagcgggcg gtttgctgat gatggccact
    14041 gctggctatg tgcaggcaga tgcgctccag cctgatccag catggcaaca ggggacgctt
    14101 tccaacggtt tacagtggca agtgctgact acgccccaac gtcccagcga tcgtgttgaa
    14161 attcgcctgc tggttaatac cggttcgctc gccgaaagta cacaacagag cggttacagt
    14221 cacgccatcc ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc gcaggcgcgt
    14281 tcattgtggc agcaggggat cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat
    14341 gacaccacgc tgtttaatct gagtttgccc aataaccgta acgacttgct gaaagaggcg
    14401 ctctcttatc tggcaaatgc cactggcaaa ctgactatca cgccagaaac catcaaccat
    14461 gcgttgcaaa gtcaggacat ggtggcaacc tggcctgccg atactaaaga gggctggtgg
    14521 cgttatcgtc tgaaaggatc aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa
    14581 cccgttgaag cggaaaagat taaagatttc tatcagaaat ggtacacccc ggatgcaatg
    14641 acgctgctgg tggtgggaaa cgtggatgcg cgctcggtcg tcgaccaaat caataaaacg
    14701 tttggcgaac tgaaaggcaa acgtgaaaca ccggctccgg tgccgacgct ttctccgctg
    14761 cgtgcggaag cggtgagtat tatgaccgac gcggtgcgtc aggaccggct atctatcatg
    14821 tgggatacgc cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg
    14881 gacctggccc gtgaggcgct gttctggcat gttcagcaag cgttaagcgc cagtaacagt
    14941 aaagacatcg gtcttggatt tgactgtcgt gtgctgtatc tgcgtgcgca gtgtgccatc
    15001 aacatcgaat caccaaacga caagctgaac agcaacctta atctggtggc gcgtgaactg
    15061 gcgaaggttc gcgataaagg tctgccggaa gaagagttca atgcgttagt ggcgcaaaag
    15121 aaactggagc tgcagaaact gtttgccgcc tatgcgcgag ctgataccga tattctgatg
    15181 gggcagcgga tgcgttcgtt gcaaaatcag gtcgtcgata tcgcgccgga gcaatatcag
    15241 aaactgcgtc aggatttcct taacagcctg acggtggaga tgttaaatca ggatctgcgt
    15301 cagcagttgt cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga gccggaattt
    15361 aacatgaaag cgttgcaggc ggcctgggat caaatcatgg ccccatcgac tgcggctgcc
    15421 gccacatctg tcgccacgga tgacgtacat cctgaagtga cggatattcc acctgtacag
    15481 taatgcctga tggtgatgag ttagcacaac catcaggtat ttatggatga gctggataga
    15541 ggggtaacac tctatccagc cttttgcata tgctgctttt acgctggcat cgcttcacgc
    15601 gggataatcg cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgct
    15661 gcgtcttccg cactgccgcc cgtcaaacgt accgccagat aaccggcact gaaagagtcg
    15721 ccagctgcgg tggtatcgat cactttttct ttcggcagtt ttaccgccgg aacatccact
    15781 aacccttcgc cagcaatgga caccaggcaa gaatccgccc cgcgtttcac caccacttct
    15841 ttcacgcccg cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg accccacagc
    15901 gcgtcttcat cgtccagcgt caggaaggcg atatccgtgc attccagcat ttgttggtac
    15961 acctgctgtg tctcttcttt gctggcccac aggcgcggac gatagttatt gtcgaaaatc
    16021 acttttccgc cgttggcgcg gcattcgcgc agcagggaaa gcagcttttc gcggctggtc
    16081 gggcttaaga tcgccaggct aatcccgctc aggtagagat aatcgaaatt cgccagctct
    16141 tcgcaaatcg ccgcggattg ctcactctcc agccagaatt tggcggcggc ttcgttacgc
    16201 cagtagtaga acgtacgttc gccggtgctg tcggtttcaa tgtagtaaag gcccggcaga
    16261 cggttttcca tccgttgggt cagggaagta tcaacgttct cgccgcgcca ggcgtccagc
    16321 atctgctggc taaaactgtc cgttcccagc gccgttacgt aatgaacggt taatgccgca
    16381 ggatcgacct gacgggcgat atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc
    16441 ttaacgtccg cgcctttctc ggaaagctca atcatgcatt cgccaatcac ggcaatcttt
    16501 ttggacatag tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt
    16561 tgctggtcaa ccatattaaa acagtgttcc attatttttt tgagccagaa cgtattcctg
    16621 aaagattccg ttgtggagtg gggaaattta ccccgccagg cggtaaagtt ctgcccttgc
    16681 gcgccgataa tctttgtcga gtccgggcag catcactttt aaacacagga catctttgat
    16741 gataaggcag gttatccagc gaataagcaa ccctgaagca agcatcgaga gcttgcagga
    16801 acggcgtttt tggttgcagt gtgagcgtgc ttacacctgg cagccaatct atcaaacatg
    16861 cgggcggtta atggccgtgg agctattaac ggtggtcatg catcccttga acccttcgca
    16921 acgcctgccg ccggatcgct attttactga aatcaccgtt agccatcgga tggaggttgt
    16981 gaaagagcag attgatttgc tggcgcaaaa agccgacttc tttgtagagc acggcctgct
    17041 ggcatcggtc aatattgatg gccctacgct catcgccctg cgtcagcaac caaaaatcct
    17101 gcgccagatt gaggatcttc cctggctgcg tttcgaactg gtggaacaca tccgcctgcc
    17161 gaaagattca acctttgcct cgatgtgtga atttggcccg ctgtggctgg atgattttgg
    17221 taccgggatg gcaaatttct ctgcactaag tgaagtgcgt tatgactaca tcaaaatcgc
    17281 gcgagaactg tttgtgatgc tgcgtcagtc accggaagga cgcacactct tttctcagct
    17341 tttacatcta atgaatcgct attgtcgcgg ggtgattgtc gagggcgtag aaacaccgga
    17401 agagtggcgt gatgttcaga actcgcccgc attcgccgca caaggctggt ttctttcacg
    17461 cccggcaccg atagaaacgc tgaatacggc ggttctggcg ctataagctg cctcattttc
    17521 cgcctggctg gactatcttt aggactggca caggaagaat atgaggaagc gaacgatgag
    17581 caaggcaggc aaaataaccg ctgcgatttc aggggctttc ttgttgttga ttgtcgtggc
    17641 gatcattttg attgcaactt ttgactggaa tcgactcaaa ccgaccatca accagaaagt
    17701 ctctgcggag ttgaatcgtc cgttcgctat ccgtggcgat ctgggcgtgg tgtgggagcg
    17761 gcaaaaacaa gaaactggct ggcgcagctg ggtgccgtgg ccccatgtac acgcggaaga
    17821 catcattctt ggcaatccac cggatattcc cgaagtcaca atggtgcatt tgccgcgagt
    17881 agaagcgacg ctggccccgc tggcgctgct gaccaaaacg gtctggttgc cgtggatcaa
    17941 gctcgaaaag cccgacgcgc gcctgattcg cctctccgaa aagaacaata actggacgtt
    18001 taatctcgcc aacgatgaca acaaagacgc gaatgcaaag ccgtcggcgt ggtcgtttca
    18061 gctggataat attcttttcg atcaagggcg gatcgccatt gatgacaaag taagcaaagc
    18121 ggatctggaa atttttgtcg atccgttagg caagccgctg ccgttcagcg aagttaccgg
    18181 atcgaaaggt aaagcggata aagaaaaggt gggcgattac gtttttggcc tgaaggcgca
    18241 gggacgttat aacggtgaac cgctcactgg tacgggaaaa ataggcggta tgctggcgct
    18301 gcgtggcgaa ggaacgccgt ttccggtaca ggctgatttc cgctctggta acacccgtgt
    18361 tgcttttgat ggcgttgtga atgacccaat gaagatgggg ggtgtcgatt tacggcttaa
    18421 attttctggc gattcgctgg gtgatctcta tgaactgacg ggcgttctgc tgcccgatac
    18481 accgccgttt gaaacggatg gtcggctggt agcgaaaatc gacactgaaa aatcgtcggt
    18541 ctttgattat cgcggtttta atgggcgcat tggcgatagc gatatccacg gttctctgat
    18601 ctacaccacc ggcaagccac gaccaaaact ggaaggtgat gtcgagtcgc ggcaattgcg
    18661 gctggcggac ctgggaccgt tgattggcgt tgattccggg aaaggggcag aaaagtcgaa
    18721 acggtctgaa cagaagaagg gcgaaaaaag cgttcagcct gcgggcaaag tgctgcctta
    18781 tgaccgcttc gaaaccgata aatgggacgt tatggatgcc gatgttcgct tcaaagggcg
    18841 gcgcattgag catggtagca gcctgccgat tagcgatctt tctactcata tcatcctcaa
    18901 aaatgctgac ctgcgtctgc aaccgctgaa atttggcata gcgggcggca gcattgcggc
    18961 gaatattcat ctggaaggcg ataaaaagcc gatgcagggg cgggcagata ttcaggctcg
    19021 tcgactgaaa ctgaaagaac tgatgcccga tgtagaactg atgcagaaga cgctggggga
    19081 aatgaacggt gacgcggaac tgcgcggtag cggtaactcg gtggcggcgc ttttaggcaa
    19141 cagtaacggc aacctgaaac tgttgatgaa tgacgggctg gtgagccgca acctgatgga
    19201 gattgttggg ctgaatgtcg gcaactacat tgtcggtgcg atatttggtg atgatgaggt
    19261 gcgggtgaac tgcgcggcgg cgaatctgga tattgccaac ggcgtggcgc gcccgcagat
    19321 ttttgctttc gatactgaga acgcgttgat taacattacc ggcacggcaa gttttgcttc
    19381 ggaacagctg gatttgacta ttgatccgga gagtaaaggg attcggatta tcacactgcg
    19441 ttcgccgctg tatgtgcgtg ggacgtttaa aaatccgcag gctggggtga aagccgggcc
    19501 gttgattgct cgcggagccg ttgcggcggc actggcaacg ctggtaacac cggcggcggc
    19561 gttactggcg ctgatctcac cttccgaagg ggaggctaat cagtgcagga caattttgtc
    19621 gcagatgaga aagtgatgat aattgccgga tgtctgacat ccggcagtta aaacgactac
    19681 aacgactggt gccgcgtctc atgcgttaac agcagggcaa ttaacgtcag tccggccatc
    19741 gccgccagat ataaccccac cgcgcctaac ccatagttag tctgcaacca ggctgcgata
    19801 tacggcgcaa cagacgcccc gagaatcgac gatacgttat aggagaacga tgctccggta
    19861 taacggactt ccgtcgggaa cagctccggc agcagcgccc ccatcggacc aaaggtcagg
    19921 cccatcaaac tcaagcccag cagcaggaaa acaaacacca atgctggatt accagaaccc
    19981 aacagtgggt taaaggcaaa cagggcaaac agaatgataa gcgtagtgat caccaccata
    20041 cttttacggc gaccaaaagc atctgccagc agacccgcca acggaaccat cactgcaaaa
    20101 ccgataaccg ccatcatcaa catccacagt acttcgttac gcggtaaacc aagcccaact
    20161 ggcgcggcgg cggtactaaa ggtcatagag tagaccgtca tgatgtaaaa cagcgtatag
    20221 gttgccagca taatgaacgt acccagtacg gttacgcgaa catgtttggt cagcagcgta
    20281 cccagcggga tcttcacctg ttttttcgct ttagcgactt tctcaaacac cggcgactca
    20341 tgcagcgaca cgcgaacata caggccgata atgaccagca ccgccgagaa gataaatggc
    20401 acacgccagc cccagctcat aaactgctcg tcggtcagta gccaggaaag cagcaagaaa
    20461 gtgccgttgg caaagaagaa gccaatcggt gcgcccagct gcgggaagga gccgtacaat
    20521 gcacgtttgc gcggtggggc gttttcagtc gccagcagcg ccgcgccgcc ccattcacca
    20581 cctaagccca gaccctgacc aaatcgagcc agcgccagca gcagcggggc gaaaatacca
    20641 atcgtggcat agcctggcag cagaccaatc accacggtcg aaatccccat cgttagcaac
    20701 gaggcgacca gcgtcgcttt acgcccaacg cgatcgccaa aatgaccaaa aacggcagag
    20761 ccaatggggc gcgcgacgaa ggcgatggca aaggtggcga gcgactgtag cgttgctgcc
    20821 gttggatcgc cctgcgggaa gaagatatgc ggaaacacaa taacggccgc agtggcgtaa
    20881 atgtaaaagt cgaagaactc aatggccgtg ccaatgagag aagcgacaag gactttatta
    20941 cgcgagttga tcggcgtgta ttcttgctcg tggtcgagtg ttgtggctgt tgcttgcata
    21001 atcttttctt attttttggc taacgaatag ccatattacg cacagcaaaa gtgatatttc
    21061 aatgtgtaac aaatcgcgga aatggtcaaa aaacaggcaa aaaggggata atgtttaggc
    21121 cagtgatttt tcttctatga aatgcttcac ataattttat aaatggtgga ttaaactggt
    21181 tttaggttaa ataaaagtta ccaactggat ttatatgctg gggttatgag ttggaataaa
    21241 attgtttgct cctgaataga tggctaaaga ggctctggga aagagtttca agaggttatg
    21301 tgcgcattat agccggatgc gatgctggcg catcttatcc ggcctacggg gagccaggcc
    21361 gaataaattc gtacgccgca tccggcctat tttaaggctg cgttttcccc ggcattcgcg
    21421 ggtcgtcttt atattcggcg gtggcaatcc acgccgcgca aaacagcgtc aaacgggcga
    21481 agaagtagaa aaatgccatc agccccagca cggaaccaaa tgctgcgcca gagggtgatt
    21541 tcatcaacga tggcagggtg taggtcatca cgattttaat cacttcaaaa ccaatagcag
    21601 ccagaaatgt tccgcggatc agggcttttt tgcgcggacg gtggcgcggc aaccgccaga
    21661 agatccagaa gaaaagcaga tagttggcga agatggaaat cgccaggcca atcaaccgcc
    21721 atgtcggttt cagccactca atgctgttca ggtgcagggc gctaataatc atttgctgcg
    21781 cagaaccggc aaccgaggtg atagaaagcg tcacaatcag cgcaatcaac aaaccaatca
    21841 gcgaaataaa atcacgcaga tattttaccc agaacttctc ctgatcttgc ggcgagcgtt
    21901 cccagacatc gcgtgactgg gcgcgaatcg cttcacgcag gttacccatc cagttgatgc
    21961 cggaataaag cgccactgcc aggccgacaa gccctacagt cgtacgctgc tgaacggcgg
    22021 tgttgatggt gtttttcaac gtggcggcta gcgtcggatc gctaatgttt tgcagaattt
    22081 tgtcgaagat atcctgtagc aacatcggat gggaggccag cacaaagccc cctgcggcaa
    22141 acgacaccat caaaatgggg atcatcgaca aaaatgagaa ataggtgata gccgccccaa
    22201 actggttacc cagccgatcg ttaaagcgtt ctgtcgcgcg aatcaggtgc gcaataaccg
    22261 gttggcgttg gactttctcg gcagtggtgg tgacggtttc cagcgcctgg ctaactttgc
    22321 cgcctttctc gctattatcc aactgcttaa tcggctcgtg ctccagatcc tgggtgggac
    22381 gtttgatctc gttttcctgc gtcatccgct cttttgtcct tctgtcgtgg tcattcttaa
    22441 aaagtatagt cggtcagtcc aggtgctctt tcattacgcc agccagccac tgcataaaca
    22501 ggtttacacg ccgggaaagc tcccgacgct gcggataaac cagtgaaagg gacagcggct
    22561 cggcacggta gccaggcaac acttcaataa gccgcccggc acgcagggct tcgcgcacgg
    22621 caatgcgcgg gatctgaata atccccagac cggcaagacc ggcggcgaga tagttttcgc
    22681 tactgtttac cgtcaacatg ccgccagact taaaccactg gacgccatta acgctggcaa
    22741 cctcaaaacc taacggatgt acacccaggt gcggtgtata acgcactatt gcgtgtgaag
    22801 tcagatcatc ggggctttgc ggataaccaa agcgcgtcag atagtgtgga ctggcacagt
    22861 tgaccatggt cagtttgccg agagaacggg cgataacgcc atcttccggt aatgcgccag
    22921 tgcgtatcac gcaatcaaaa ccatcatgaa gaatatctac cggacggtca tggctactca
    22981 gttccagctc aattcccgga tgcagataga gaaattccga caagcgcggc agtaacaggc
    23041 ttttcgcgat tcctgacggg atgtcgatgc gtaatttacc gctgatactg gtggcatcct
    23101 gttgaaacag accgtccagt tcgctgagat tactcaacac atcttttgct cgttgataat
    23161 aggtcatgcc ttctggcgtg agtttgaccc gtcgcgtggt gcgctggaga agctgggtgc
    23221 caagttgatg ttccagtgcc tgtatctggc gcgaaacact tccctttggc aaagcaaaga
    23281 aatccgccgc gcgggaaaaa ctttccagct ccgcgacttt gatgaacaac tgcattgcgt
    23341 gaattttatc catttctttg accgattgtt gttcacaacg aaacagtagc gcgcatatcg
    23401 cattgtttat tgttttttct aacatctaat gccgggtttc acaatgttgt catgatgaat
    23461 gtggcgattt ctcagcagga aaagggagga atagctttat gagaaaggtg aattccctta
    23521 aacagagcgt atttttcagc gattcccttt aaagtgaata gaagatgtat tggctatttg
    23581 tgcttatttt tatctcattg ccgtttgtta tttttgttta tggtcgcgga atttcatttt
    23641 taattgcatg cgtactaact accgatataa ggaaatcctc aaaaatgttt taaatagtgc
    23701 cctcgacatg taatttcagg cgtttagctg taactattta tatcggtaac tattttctac
    23761 gcgttatctc tgtagaatgg ccaccgccaa atggcgaatt cttatttttt ctcgcgggta
    23821 atacccttcc actacttaag tctttttaac gtattcaggt ggagattcag ttatgcaaat
    23881 agtcatgttt gacaggcagt caatatttat tcatggaatg aaaatcagtt tacagcatcg
    23941 tattccagga gtgagtattc agggggccag tcaggcagac gagttatggc aaaagctgga
    24001 aagttaccct gaagccttag tgatgctcga tggcgatcag gatggtgagt tttgctactg
    24061 gttgctgcaa aaaaccgcgg tgcaatttcc tgaggttaag gtgttaatta cggcgacgga
    24121 ttgcaacaaa cggtggttac aggaagttat tcattttaat gtgctggcca ttgtgcctcg
    24181 tgattcaacc gtcgagacat ttgcgttggc ggtcaatagt gcggcgatgg ggatgatgtt
    24241 tctgcctgga gactggcgaa cgacgccgga aaaggacatt aaagatctca aatcgttaag
    24301 cgcccgccag cgggagattt taaccatgtt agcggcgggc gaatcaaata aagagatcgg
    24361 cagggcgctg aatatcagta ccggaacggt aaaagcgcat ctggaatcac tgtatcgccg
    24421 tctggaagtc aaaaatcgca cccaggcggc aatgatgtta aatatctcct cctgaagagg
    24481 ataattttac agccagcagg aagttagcgg ctggctgtaa aaatattatg gttcgccgta
    24541 caaaccaatt aaacggcgta ccacaccgtt agtccagcca aacccatcct gcaacggata
    24601 ctcgccaccg ccgccttcgc gaggaacacc atcggcaata tggtatttct cgatcagttt
    24661 gtgctgttcc agatagaact gattcaccgt cttcagccag ttgcgcgcga tttcatcacc
    24721 cagaaggtca tcgccgtaca tcttaaatcc ctgaattgcc atccattgta acggtgccca
    24781 gccgttgggt ttatcccact gttcaccggt ttcgtactcg cttgccagaa tcccgccagg
    24841 tgtcagtaac cggctgcgca cggcgtttgc cagacgatcg gcctgttcat ggttcgccat
    24901 accgacatag agcggcacaa tggcggcagc ggaaaacagc gccagttgct cgcgtcgcca
    24961 gtcgtaatcg cggtagatgc cgttttcatc atcccagagg taacggttta ccgcatcgcg
    25021 acgggcactg gccttctggc ggaacagtgc ttctgtctct ttctcgcctt tcagcgccga
    25081 gatgttggcg atggcgctct ccagtttaaa caggaaggca ttcagatcga tggggatgaa
    25141 ctgggtggta cgaatgctcg ccagacgacc agtatcacgc agccaacggg aagagtaatc
    25201 ccaaccggag gccgcccccg cacgtaaatc gcggtacacc tcgttaggcg ggcgaccaga
    25261 atgtttcgcg gtttcaacgt cctcaagcca ggattcgtca cgcggcgtgt cgcgatcgtc
    25321 ccagtaacgg ttgagcagcg agccgtccgg catccgcaca acatggcgat aggcctgatt
    25381 agggatcaac gattctgcac cgtccatcca gaaggcatat tccattttca ggtggtcgag
    25441 atagcggcgc gcgccgcgta caccatcttc ttcaaacaac tccaccatca gcgcaaaaac
    25501 cggtggttgc gagcggctca aataataggt gcggttgccg ttggggatgt ggccgtagtt
    25561 ttcgatcatc caggcgaagt tatcggccat gcatttcagc aaatcttccc gaccactttc
    25621 cgccagcccc agcatggtga aataggaatc ccaatagtag gtttcgctaa aacggccgcc
    25681 cgggacaata tatgactgcg gcagcgccag cagagaagac cacggaatgt gatcctgtgg
    25741 ttcacgggtt agcaccggcc acagctggtc gatatgctct ttcagggaat tttgcgggtc
    25801 cgatacatac tcgctggagt agacctccgg cagccagaag tggttttcaa caaacttgcg
    25861 caagtcaaaa tcacgatgac ggcgcacttt acggtagcgg attaaaatat ccagcgggtc
    25921 cattttgggt gcgcagtcgg gaaaggtttt actgtcgggg aaaattttcg ccgactgaac
    25981 atgctcgaac agttcgagat agcgatcggc aggcgtcagc gcatcagagg cgggcagccc
    26041 ttcaatcatc tcgggttccg gttctgcctc gatcatctca tccagtttta attcatacgg
    26101 gtccagctca tagcagagat cgacctcgat catcagttcg tctggattag ggttttgaat
    26161 tttctgattg agcataagga tccgacctcc gaaattcgtc gctaattatc gggcttttct
    26221 ttaagggtag tcattcgctc cgcctggtgg gcgacaaagc gtgcggtgga tcacgtttat
    26281 taacctgcca aaaaatataa cgcactaata tcaatggaat ttataaaaat acggatttcg
    26341 tgttgttttt cattttcaga acattcaatc ctgctctcgc cgcaatcatg atatgccatt
    26401 cctgatctgg gtaataggca ctggctatct tattgatagt ttatattcat gtaattgatt
    26461 gattcctatc aaaaagcata tctccctgtt ttttatgatg gctttgtctc gcagagacgt
    26521 ttgcagcatt ctggaaaatt ttctgggtca caataactca ccaccgtgag ggaagaataa
    26581 tgaaaatggt ctcacgtatt accgcgatcg gcctggctgg cgtcgcgatt tgctatttag
    26641 ggttatctgg ttatgtgtgg taccacgata ataaacgcag taaacaggcc gatgttcagg
    26701 catctgctgt cagtgaaaat aataaggttt taggctttct ccgcgaaaaa ggatgcgatt
    26761 attgccatac gccttcggca gaattacctg cctattatta tattcccggc gcgaaacagt
    26821 tgatggatta cgacataaag cttggatata aatcttttaa cctcgaggcc gtgcgtgcag
    26881 ctctgctggc taataaaccc gtttcgcaaa gcgatctgaa taagattgaa tgggtgatgc
    26941 agtatgaaac tatgccgcca acgcgttata ccgcgctaca ctgggcgggt aaggtgagtg
    27001 atgaagagcg ggcggaaata ctggcctgga ttgcaaaaca gcgcgcggaa tattacgcca
    27061 gcaatgatac tgctccggag catcgcaatg aaccggtgca gcccatcccg caaaaactgc
    27121 ctaccgatgc gcaaaaagtg gcgttgggtt ttgcgctgta tcacgatccc cgtttatcgg
    27181 ctgatagcac catttcatgc gctcattgcc atgcgttgaa tgcggggggc gtcgatggca
    27241 gaaaaacatc gattggtgtt ggtggcgcag ttgggccgat taacgcgccg acggtattta
    27301 actcagtatt taacgttgag cagttctggg atggtcgtgc ggcaacattg caggatcagg
    27361 ctggtggacc gccgttgaac ccgattgaaa tggcgtcgaa atcctgggac gaaattattg
    27421 ctaagctgga aaaagatccg cagcttaaag cgcagttcct cgaagtctat ccgcaaggtt
    27481 tcagtggcga aaatattact gatgccattg ctgaatttga gaaaacatta attacgccgg
    27541 attccccatt tgatagatgg ttgcgtggag atgagaatgc tctgacggcg caacagaaaa
    27601 aaggctatca attatttaaa gataataaat gtgcaacttg tcatggtggt attattctcg
    27661 gcggacgttc ctttgaaccg ttggggctga aaaaagactt taactttggt gaaattacgg
    27721 cggcggatat tggtcgtatg aatgttacta aagaagagcg tgataaattg cgtcagaaag
    27781 tacccggttt acgtaacgtt gctttaacgg caccgtactt ccatcgcggt gacgtgccga
    27841 cgctggacgg ggcggtgaaa ctgatgctgc gctatcaggt aggcaaagag ctgccgcagg
    27901 aggatgtgga tgatatcgtc gctttcctgc acagtctgaa cggggtttac acgccgtata
    27961 tgcaggataa acaataatta atttgatcgc ccgaacagga gtgtttgggc gatttttatt
    28021 acgataataa agtctgtttt taatattatc atattaaatg tttatattat aaaaagtcgt
    28081 ttttctgctt aggattttgt tatttaaatt aagcctgtaa taccttgctt ccattgcgga
    28141 taaatcctac ttttttattg ccttcaaata aatttaagga gttcgaaatg gaccagaagc
    28201 tgttaacgga tttccgctca gaactactcg attcacgttt tggcgcaaag gccatttcta
    28261 ctatcgcgga gtcaaaacga tttccgctgc acgaaatgcg cgatgatgtt gcatttcaga
    28321 ttatcaatga tgaattatat cttgatggca acgctcgtca gaacctggcc actttctgcc
    28381 agacctggga cgacgaaaac gtccataaat tgatggattt gtcgatcaat aaaaactgga
    28441 tcgacaaaga agaatatccg caatccgctg caatcgacct gcgttgcgta aacatggttg
    28501 ccgatctgtg gcatgcgcct gcaccgaaaa atggtcaggc tgttggcacc aacaccattg
    28561 gttcttccga ggcctgtatg ctcggcggga tggcgatgaa atggcgttgg cgcaagcgta
    28621 tggaagccgc aggcaaacca acggataaac caaacctggt gtgcggtccg gtacaaatct
    28681 gctggcataa attcgcccgc tactgggatg tggagttgcg tgagatccct atgcgccccg
    28741 gtcagttgtt tatggacccg aaacgcatga ttgaagcctg cgacgaaaa
//
LOCUS       DAZFFE010000055        28425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFFE010000055 DAZFFE010000000
VERSION     DAZFFE010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.055"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..368)
                     /locus_tag="ACHJ69_002573"
     CDS             complement(<1..368)
                     /locus_tag="ACHJ69_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO5015532.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLG"
     gene            complement(439..2553)
                     /gene="fusA"
                     /locus_tag="ACHJ69_002574"
     CDS             complement(439..2553)
                     /gene="fusA"
                     /locus_tag="ACHJ69_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO5015533.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2650..3120)
                     /gene="rpsG"
                     /locus_tag="ACHJ69_002575"
     CDS             complement(2650..3120)
                     /gene="rpsG"
                     /locus_tag="ACHJ69_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO5015534.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3217..3591)
                     /gene="rpsL"
                     /locus_tag="ACHJ69_002576"
     CDS             complement(3217..3591)
                     /gene="rpsL"
                     /locus_tag="ACHJ69_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO5015535.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3717..4004)
                     /gene="tusB"
                     /locus_tag="ACHJ69_002577"
     CDS             complement(3717..4004)
                     /gene="tusB"
                     /locus_tag="ACHJ69_002577"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO5015536.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGRISNDIISIDYTDFVRLTVKHSSQMAW"
     gene            complement(4011..4370)
                     /gene="tusC"
                     /locus_tag="ACHJ69_002578"
     CDS             complement(4011..4370)
                     /gene="tusC"
                     /locus_tag="ACHJ69_002578"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO5015537.1"
                     /translation="MKRIAFVFSTAPHGTTAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWICAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4370..4756)
                     /gene="tusD"
                     /locus_tag="ACHJ69_002579"
     CDS             complement(4370..4756)
                     /gene="tusD"
                     /locus_tag="ACHJ69_002579"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO5015538.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4756..5478)
                     /gene="yheO"
                     /locus_tag="ACHJ69_002580"
     CDS             complement(4756..5478)
                     /gene="yheO"
                     /locus_tag="ACHJ69_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5015539.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5645..6457)
                     /gene="fkpA"
                     /locus_tag="ACHJ69_002581"
     CDS             complement(5645..6457)
                     /gene="fkpA"
                     /locus_tag="ACHJ69_002581"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO5015540.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKQEADAKAADSAKK"
     gene            6678..6896
                     /gene="slyX"
                     /locus_tag="ACHJ69_002582"
     CDS             6678..6896
                     /gene="slyX"
                     /locus_tag="ACHJ69_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO5015541.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6945..7535)
                     /gene="slyD"
                     /locus_tag="ACHJ69_002583"
     CDS             complement(6945..7535)
                     /gene="slyD"
                     /locus_tag="ACHJ69_002583"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO5015542.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7630..7830)
                     /gene="yheV"
                     /locus_tag="ACHJ69_002584"
     CDS             complement(7630..7830)
                     /gene="yheV"
                     /locus_tag="ACHJ69_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO5015543.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7840..9645)
                     /gene="kefB"
                     /locus_tag="ACHJ69_002585"
     CDS             complement(7840..9645)
                     /gene="kefB"
                     /locus_tag="ACHJ69_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO5015544.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTHFSWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9645..10196)
                     /gene="kefG"
                     /locus_tag="ACHJ69_002586"
     CDS             complement(9645..10196)
                     /gene="kefG"
                     /locus_tag="ACHJ69_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO5015545.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFINIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGC"
     gene            10327..12240
                     /gene="yheS"
                     /locus_tag="ACHJ69_002587"
     CDS             10327..12240
                     /gene="yheS"
                     /locus_tag="ACHJ69_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5015546.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIY
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12240..13262
                     /gene="yheT"
                     /locus_tag="ACHJ69_002588"
     CDS             12240..13262
                     /gene="yheT"
                     /locus_tag="ACHJ69_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO5015547.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13256..13474
                     /gene="yheU"
                     /locus_tag="ACHJ69_002589"
     CDS             13256..13474
                     /gene="yheU"
                     /locus_tag="ACHJ69_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO5015548.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13528..14397
                     /gene="prkB"
                     /locus_tag="ACHJ69_002590"
     CDS             13528..14397
                     /gene="prkB"
                     /locus_tag="ACHJ69_002590"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO5015549.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQSLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFS
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14452..14856)
                     /gene="yhfA"
                     /locus_tag="ACHJ69_002591"
     CDS             complement(14452..14856)
                     /gene="yhfA"
                     /locus_tag="ACHJ69_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO5015550.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15158..15790
                     /gene="crp"
                     /locus_tag="ACHJ69_002592"
     CDS             15158..15790
                     /gene="crp"
                     /locus_tag="ACHJ69_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO5015551.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15841..17931
                     /gene="yhfK"
                     /locus_tag="ACHJ69_002593"
     CDS             15841..17931
                     /gene="yhfK"
                     /locus_tag="ACHJ69_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO5015552.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQVLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVTLHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEYPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17998..19218)
                     /gene="argD"
                     /locus_tag="ACHJ69_002594"
                     /gene_synonym="dapC"
     CDS             complement(17998..19218)
                     /gene="argD"
                     /locus_tag="ACHJ69_002594"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO5015553.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPVSAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEEGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAVAKVIGA"
     gene            complement(19304..19867)
                     /gene="pabA"
                     /locus_tag="ACHJ69_002595"
     CDS             complement(19304..19867)
                     /gene="pabA"
                     /locus_tag="ACHJ69_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO5015554.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPECFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19899..20501)
                     /gene="fic"
                     /locus_tag="ACHJ69_002596"
     CDS             complement(19899..20501)
                     /gene="fic"
                     /locus_tag="ACHJ69_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO5015555.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTLHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQNGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20491..20658)
                     /gene="yhfG"
                     /locus_tag="ACHJ69_002597"
     CDS             complement(20491..20658)
                     /gene="yhfG"
                     /locus_tag="ACHJ69_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO5015556.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEALRRHYER"
     gene            complement(20763..21335)
                     /gene="ppiA"
                     /locus_tag="ACHJ69_002598"
                     /gene_synonym="rotA"
     CDS             complement(20763..21335)
                     /gene="ppiA"
                     /locus_tag="ACHJ69_002598"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO5015557.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21606..22787
                     /gene="tsgA"
                     /locus_tag="ACHJ69_002599"
     CDS             21606..22787
                     /gene="tsgA"
                     /locus_tag="ACHJ69_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO5015558.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAYMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23049..25592
                     /gene="nirB"
                     /locus_tag="ACHJ69_002600"
     CDS             23049..25592
                     /gene="nirB"
                     /locus_tag="ACHJ69_002600"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO5015559.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25589..25915
                     /gene="nirD"
                     /locus_tag="ACHJ69_002601"
     CDS             25589..25915
                     /gene="nirD"
                     /locus_tag="ACHJ69_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO5015560.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYDARVKDG
                     VVQLRG"
     gene            26041..26847
                     /gene="nirC"
                     /locus_tag="ACHJ69_002602"
     CDS             26041..26847
                     /gene="nirC"
                     /locus_tag="ACHJ69_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO5015561.1"
                     /translation="MFTDTITKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26866..28239
                     /gene="cysG"
                     /locus_tag="ACHJ69_002603"
     CDS             26866..28239
                     /gene="cysG"
                     /locus_tag="ACHJ69_002603"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO5015562.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEYGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6895 a         7616 c         7169 g         6745 t
ORIGIN      
        1 cccagcagga tgtgctcacg agtctgcggc atcgggccgt cagtcgcagc aactaccagg
       61 atcgcgccgt ccatctgagc agcaccggtg atcatgtttt taacatagtc ggcgtgcccc
      121 gggcagtcta cgtgcgcgta gtgacgggtc ggggtgtcgt attcaacgtg agaagtgttg
      181 atggtgatac cacgagcttt ttcttccggc gcgttatcga tctggtcgaa tgcacgagca
      241 gcaccgccgt aggttttagc cagtacggtg gtgattgcag cggtcagagt agttttaccg
      301 tggtcaacgt ggccgatagt accaacgtta acgtgcggtt ttgtacgttc aaatttttct
      361 ttagacacgg ctatattcct tactatagtg ctctcccctt caggagagag cacgggactt
      421 tggtattaac ccttaggctt atttaccacg ggcttcaatt acggcctgag caacgttact
      481 cggcgcttca tcatacttca ggaattccat agtgtatgat gcgcgacctt tggtcagaga
      541 acgcagctga gttgcatatc cgaacatttc agacagcggt acttcagcgt ggatcttaac
      601 gccagtaact tcagattcct gacctttgag cataccacga cgacggctca agtcaccgat
      661 aacgtcaccg gtgttctctt caggagtttc tacttcaacc ttcatgatcg gctcaagcag
      721 aactggtttc gctttcttaa agccttcttt aaaggcgata gaagcagcca gtttaaacgc
      781 cagttcagag gagtcaacgt catggtaaga accgaagtgc agacgaatac ccatgtctac
      841 taccgggtag cctgccagcg gacctgcttt cagctgttcc tggatacctt tatcaacggc
      901 cgggatgtat tcgccaggga ttacaccacc tttaatgtcg ttgatgaact cgtagccttt
      961 cgggtttgaa cccggctcca gcgggtacat gtcgataaca acatgaccat actgaccacg
     1021 accaccagac tgtttcgcgt gtttaccttc aacatcggta actttctggc ggatagtttc
     1081 acggtaagca acctgcggtt tacctacgtt cgcttcaacg ttgaattcac gcttcatacg
     1141 gtcaacgatg atgtcgaggt gcagttcgcc catacccgcg atgatggtct ggttagattc
     1201 ttcgtcagtc catacacgga aagacgggtc ttctttagcc agacggccca gagccagacc
     1261 cattttttcc tggtcagctt tggttttcgg ttcaactgcg atggagatta ccggctcagg
     1321 gaattccata cgttccagaa tgatcggcgc atccgggtca cacagggtgt caccagtggt
     1381 tacgtctttc agaccgattg cagctgcgat gtcgcccgcg cgaacttctt tgatctcttc
     1441 acgtttgtta gcgtgcatct gaacgatacg accgaaacgc tcacgtgcag ctttcacgga
     1501 gttcagtacg gtatcaccag agttaaccac accggagtac acacggaaga aggtcaggtt
     1561 accaacaaac gggtcggtag cgattttgaa cgccagtgca gagaacggct cgtcatcact
     1621 tgcgtgacgc tcagccggag tgtctttacc gtcgtccagg ataccgttga tcgcaggtac
     1681 gtcaaccggg gatggcaggt aatcaattac cgcatccagc atcgcctgaa cacctttgtt
     1741 cttgaacgca gaaccacagg ttaccaggat gatttcgttg ttcagaacgc gctgacgcag
     1801 agcacctttg atttctgctt cagtcagttc ttcaccaccc aggtattttt ccatcagctc
     1861 ttcagaagct tcagctgcgg attcgatcag gttctggtgc cattcgttag ccagttcaac
     1921 catgtctgcc gggatatctt cgtattcgaa ggttacgccc tggtcagcgt cgttccagtt
     1981 gatagctttc attttcacca ggtcaacaac accggtgaaa tgttcttcag caccaatcgc
     2041 cagctgcagc ggaaccgggt tcgcgcccag acgggttttg atctggttaa caactttcag
     2101 gaagttcgca cccatgcggt ccattttgtt aacgaacgca atacgcggaa ctttatattt
     2161 gtttgcctga cgccatacgg tttcagactg cggctgaaca ccaccaactg cgcagtaaac
     2221 cattaccgca ccatcgagaa cacgcatgga acgttctact tcgattgtga agtcaacgtg
     2281 ccccggggtg tcgatgatgt tgatgcgatg cggctcatac tgcttagcca taccagacca
     2341 gaatgcagta gtcgcagcgg aagtgatggt aataccacgt tcctgctcct gctccatcca
     2401 gtccatggtt gcagcgccgt catgaacttc accgatttta tggtttacac cggtgtagaa
     2461 cagaatacgt tcggtagtag tggttttacc ggcgtcgatg tgcgcactga taccgatgtt
     2521 acggtagcgt gcgatgggtg ttgtacgagc catttgtttc ctcgtttatc ttttaggcgt
     2581 tcaatttaag tagcccaaag cgggctgctt actggaagcg cccgcctggt gactaaaact
     2641 ccgaagggat taccaacggt agtgtgcgaa cgccttgttg gcttcggcca tacggtgaac
     2701 gtcttcacgt ttcttaactg cagtaccttt gttttctgca gcatcagaaa gttcgttcgc
     2761 caggcgcaga gccatggatt tatcaccgcg tttacgagca gcttcaacga tccaacgcat
     2821 tgccagagca ttacgacgaa ccggacggac ttcaactggt acctgataag tagaaccacc
     2881 aacgcggcga gacttaactt ctacagtcgg gcgcacgttt tcgagagcta cttcgaatgc
     2941 ttccagttca gatttaccag agcgctgagc cagggtctcc agcgcgctgt atacgataga
     3001 ttcagcagta gattttttac catctaccat caggatattt acaaatttag ccagcagttc
     3061 tgatccgaac ttcggatccg gcagaatttt acgctgacca atgacgcgac gacgtggcat
     3121 ggaaatactc cgttgttaat tcaggattgt ccaaaactct acgagtttag tttgacattt
     3181 aagttaaaac gtttggcctt acttaacgga gaaccattaa gccttaggac gcttcacgcc
     3241 atacttggaa cgagcctgct tacggtcttt aacgccggag cagtcaagcg caccacgtac
     3301 ggtgtggtaa cgaacacccg ggaggtcttt aacacgacca ccacggatca ggatcacgga
     3361 gtgctcctgc aggttgtgac cttcaccacc gatgtaggaa gtcacttcga aaccgttagt
     3421 cagacgaaca cggcatactt tacgcagcgc ggagttcggt tttttaggag tggtagtata
     3481 tacacgagta catacgccac gtttttgcgg gcatgcttcc agcgcaggca cgttgctttt
     3541 cgcaactttg cgagcacgtg gtttgcgtac cagctggtta actgttgcca ttaaatagct
     3601 cctggtttta gcttttgctt cgtaaacacg taataaaacg tcctcacaca atatgaggac
     3661 gccgaatttt agggcgatgc cgaaaaggtg tcaagaaata tacaacgatc ccgccatcac
     3721 caggccatct ggctggagtg cttaaccgta agtctgacga aatcagtata gtcaatggaa
     3781 atgatgtcgt tcgaaattcg accagtcaaa ccgcgggcaa taaggtcttc gttcagagca
     3841 tagaccttaa tgggggcatt acgcagactt tcaaggtagc ggttgccgtc aactgcggca
     3901 gttacgccat cttgcaataa tagcagttcg tcaccttcac tgagcagacg cagcagcgca
     3961 gcaaagtccg tcagccaggg tgaacgatgt aatgtgtgca gcataacgcc tcaaaacctc
     4021 aaaataacat cgtagttggc gagttcgcgg cgtaaggcat ctgcttcgag cggcgtggct
     4081 tcgaccacaa agggtgtctg cggatctaac ccacgttcgc gcagtgaagc cgcgcaaatc
     4141 cagcactgtt caatatcgta cagacccaac aatttaaaag tggcaatgta atcacgcgcc
     4201 agcactgcat cgggcttttg tcctggcagc agctgaaaaa cgccatcagc aataaagaag
     4261 acggccagat cgtcagttaa tgcggaagtt gccagtaaag catctaaacc ttcccggcct
     4321 gcagttgtac catgaggcgc agtagaaaaa acaaacgcaa ttcgtttcat cagaactgta
     4381 ccaccctgtc acaggtcagc gaggcttccg ccagcgcgcc aagtccgctt aaggtaaatc
     4441 ccggctgaag gtttgacgaa gcgagcccca gtcttccggc ctccgtttca tcaacaacgc
     4501 cacggcgtaa tgctgccgct acgcagatat tcagcgccac accatgttgc gcattcaact
     4561 gttgccagcc gcgcacaagg tcaaattcgt cgcttgccgg tgaggtcaat tggttagcgt
     4621 tatagacccc ttcccgatag aaaaaaacgc tgcttaactc atggccttct gctatcagcg
     4681 cctgcgcaaa ctgaaaagca ctactcgcct gttgcgtacc gtatgctggc ccggtcacca
     4741 cgatggcaaa acgcattact tatcttgccc ctggaaatca ccgctcttga actggcggat
     4801 gtagagatag acagtgtgtt tggagatgtt caggcggtca gcaacctggt tgatcgcgtc
     4861 tttaatatcg aagatccctt tctcgtagag atttagcacg atctgacgat ttttggcgtt
     4921 attagaaaca ttgcgatcgg cattcacttc ttcgatggtg aactccagcg tttgggtaac
     4981 cagatcttca acagaagagg caaagttgac gcttgaaccg acatccggag tttccggcgg
     5041 cacaaaagtg ctcataatct gcgagaaggg aacatcaaga ttcatattga tgcacagcag
     5101 accaattaca cgctgttcgc ggttacgaat cgcgatagtc agggacttca ttaatacgcc
     5161 gcttttggcg cgagtaaagt agcatttaga aacgctgcta tccgctcccg tcatatcgtg
     5221 cagcatacgt agcgccaggt cagtaattgg cgaaccaatc ttccggcctg tatgttcacc
     5281 gttagcaata cgaatggctg aacattttag atcctgcaaa gagtgcaaaa cgatttcaca
     5341 gtgggagcca ataagcatcg ctaacccgtc caccaccgct tcgtaggatt tcagaatatc
     5401 aaaatcggtc tggtcaaaag gacgttgatc cagtaaatcc aactcactgg tttcgttggt
     5461 taaaagcgac ctggacatga aaaaaaacac tccttttcag gagcctgtcg ttaacttttc
     5521 agggcaggct cattaatgat gcgggtaact aaattaatac agcggaggtt ccgctttcca
     5581 gcactaatta tatccggcct gtaataaaaa aaccgccgcc tggtcaggcg gcggttctta
     5641 atgcttattt tttagcagaa tctgcggctt tcgcatcagc ttcctgcttt gcatcagcct
     5701 tcggcgctgg tttcacatcc agcagctcta cgtcaaacac cagggtagaa ttcggtggga
     5761 tccccggaac acccgctttg ccgtaagcca gttctggtgg aataaccagt ttgatcttac
     5821 cgcctttctt gatgttcttc agaccttctg tccaacccgg gataacaccg tccagacgga
     5881 aagagagcgg ttcaccacgg gtgtaagagt tgtcgaactc tttaccgtcg atcagcgtac
     5941 ctttgtagtt cactacaaca gtatcgctgt ctttcggtgc ttcgccttta ccggcttcta
     6001 ctacctgata aaccagacca gtggaagagg ttttcacacc tttctctttg gcaaatttct
     6061 cgcggtactc tttacctttt gcttcgttat cagccgcgtc tttttccatc ttcgcctgag
     6121 cagaagactt cacacgagct tcgaatgctt gcagagtctg ttcgatctct tggtcggaaa
     6181 gtttgctctt atcagcaaat gcatcctgaa caccagcgat cagctgatct ttatccagtt
     6241 tgatgcccag tttttcttgt tctttaagag agttttccat gtaacgaccc agcgaagcac
     6301 ccagtgcata agctgatttc tgatcgtcat ttttgaacgc tgctttgctg tcagcagttg
     6361 tagcaggttt tgcagcttca gcagcaaaag tgattggtgc atgcagggca acggccattg
     6421 tggtcgccag cagcgttact ttaaacagtg atttcatcca tatctccagg atcggggcat
     6481 ctcaccccag ggttaactat tatcagaagg gtactataaa gcgttgtcga acaaatctac
     6541 atacagacac gccctattat catctatttt cagactcttt ttgtttaaat tagtttcgat
     6601 gaccgcgaaa tgagtgctgt ctcgggcagc aaagttaagt agaatccgcg gcgaccattc
     6661 gacaaaagag gtgaatcatg caggatttat cattggaagc acgcctggca gagctggaga
     6721 gccgattggc ttttcaggaa atcaccattg aagaactgaa cgtcacggtg accgctcatg
     6781 aaatggagat ggcgaaactg cgcgatcatc tgcgtctgct gaccgagaag ctaaaagcca
     6841 gccagccgtc gaacatcgcg tcgcaggctg aagaaacgcc accgccacat tattgaggcg
     6901 taaaaaaagc ggggattccc cgcttttttg tcaccttttc ggtattagtg gcaaccgcaa
     6961 ccgccgttgc ctttaccgcc acagcagcct tcgccaccgt gttcatgacc gtgatcgtgg
     7021 ccatgaccac cgcagcaacc gtcgtggtcg tgatcgtggt ggtgatcgtg cgcgccgtga
     7081 acgtgaccat gagccagttc ttcttcggtc gcttcgcgaa tcgccacaac ttcaacgttg
     7141 aatttcaggt tctgaccagc cagcatgtgg ttaccatcaa ccactacgtg atcgtcttca
     7201 accgcagtga tttcaaccgg taccggaccc tggtcggttt cagccaggaa acgcatacct
     7261 acctgcagtt catcaacgcc cataaatacg tctttaggaa cgcgctgcac caggttttcg
     7321 tcgtactgac cgtaagcgtc gttcgcgcca acagcgacat caaatttgtc gccaacttca
     7381 tgaccttcca gcgctgtttc caggccagag atcagggaac cgtgaccatg caggtagtcc
     7441 agcggcgcac tcaccggaga ctcatcaacc aacacaccgt cttctgtacg tacctgatag
     7501 gccaggctga ccaccaggtc ttttgctact ttcatgatat ctcctgagca tgggaagaat
     7561 agtggcgcag attgtagcgg aattctgcag ccgtgtactc actagcttaa aaaaactcgg
     7621 cgcatatcgc tagtccggat gaaaaatccc gatcacttgc tcatctttgc ggacgtgatc
     7681 gcgggcttct ttgtctgctt ctcgcatctg atgtccacac ttaacacatt ctacgatatc
     7741 aatattattt tcgcgccaca tcgccattga atcctgtgcc tgacaggccg ggcattttgc
     7801 gcccgcaata aaacgttttc ggattgccat ctttaccctc tactcaaatt catcccagcc
     7861 gtccagctgg cgtcgttctt gttgcatttc acgctggaaa atctcttcca gttcacgtcg
     7921 ggcttccctg gcgcgagaaa tttgtacggt atcagcatgc atcgggatta actcccgcag
     7981 cattcgcata tccaggcggc gaaaatgcag ttgcgcgcgc tgcgcctgat gcggatgcat
     8041 gccaagcgtg accagcgtct tgcgccccag ctctaacgca ctggagaatg tttcacggga
     8101 aaactgcgtc acccctgcct gtaataactc atgcgcttcc acacgtccgc gcgctcgcgc
     8161 aagaatatgc aaatgcggaa agtgctgctg gcatatttcc accagcttca tagtgtcttc
     8221 cggctcgtta caggtaatga cgatagactc agcggcctct gcacccgcag aacgtaaaag
     8281 atctacctgc gtggcgtcgc cgtaataaac tttgtagcca tatttgcgca tcaggttaac
     8341 ggcactgata tcccgctcca gtacagtaat gcgcatttta tttgccatca gcaaacgacc
     8401 aatcacctga ccaaaacgcc caaagcccac gacaatgacc tgcggtttat catcgttgac
     8461 ccatggtttt tcgtcttctt cttccggtcc gttaaactgg cgggatagcc atttatcgac
     8521 cagcttcatc agcagcggcg tggtcatcat ggaaagcgtc accgtcacca gcaacagcgc
     8581 catctggtcg ccctggaata agcgttgtga agaagcggta gaaaagagga caaaggcaaa
     8641 ctcaccgccc tgactcaaca cgccagcaaa ctgcatccgc tcagaactgc gcacgccata
     8701 caatcgcgcc agcagataca gcacgagaat tttcaccgcc accagcacaa ccacacttat
     8761 cactacccac aacagatggg tataaagcac cccgaggttg agcgacatgc cgacagagat
     8821 aaagaacaaa ccaagcagca agcctttgaa gggatcgata gccgtttcca gttcatggcg
     8881 atattcactt tccgccagta gcacacccgc aataaacgta ccgagcgcca tcgacaaccc
     8941 cagcgcatcc ataaacaatg cggaacccaa caccagcagc agcgtcgcgg cggtgaacac
     9001 ttcccgcaca ccagaagctg caataaaacg gaataccgga cgcagtaaat agcgcccgcc
     9061 aatcagcatg ccgacaaacg ccagcacctt catgccgatc ttcatccagt cgaaatgttc
     9121 gtctgccgaa cccgccaaca acggcactaa cgccagtgcc gggatcaccg ccagatcctg
     9181 aaacagcagt accgagaaac ctaactggcc ggattcgctg cgattcatcc ctttctcacg
     9241 catcaattgc aacgccattg cagttgaaga catcgcaagg ccaatgccac cgaccaccgc
     9301 cgcctgccat gagaaatggg tcagcatcaa taatcctgcc agcaacgccg cgcttaacag
     9361 cacctgtgcc gcgcctacgc caaaaatcga acgccgcagt tgccaaagct tggagggatt
     9421 caactcaagg ccgatgataa acatcaggaa taccacgccg agttccgaaa agtggaggat
     9481 ctcatcgacg tcgctaataa accccagccc ccacgggcca atcgcaatcc ccgccagcaa
     9541 atatcccaac actgcaccaa tacccagccg cgatgccagc ggcaccgcag ccaccgccgc
     9601 gaagagaaac agcactcctg cgagtaaaaa atcagaacct tccatcagca gcctcctgga
     9661 gacagcggat ttgccagcca gtcaccgtag gctctggcgt ggctcgccag ctcctgtgcg
     9721 ctttgccgtc tcgcccagta aataatgatg gggcttaacc aatgcatccg gcacatgccc
     9781 gccgccagtt caaaggggcg cagaacatcg ctcatcgggt agcgattcaa cgcgtcataa
     9841 cggtaagcac tttccggctc cccggtggta atcacgctac gccagtactt tcccgccagt
     9901 tggtttcccc ccggtccgct ggcaaaacca cggcttaata cccggtccag ccactctttc
     9961 agtagcgccg ggcagctata ggtataaaga ggatgctgaa agacaatcac ctcgtgctcg
    10021 cgcagtaatg cctgctcacg agggatatta ataaaaaagt cgggatagtg cgcgtaaagg
    10081 tcgtgcacgg taacattgct gagctgcgtg gccggtttaa gcagaacccg gtttgccacc
    10141 gagtcctgag attccggatg ggcatacagc agcaaaactt tcgctggctg agacatcatc
    10201 cccctcccgg tatggttttt gtgtatagtc gctgttttgg gctaccattg cgcccggtgc
    10261 ggcagctcgc ccatacatta cattatcata atgataagtt aacatagtct gaacatacgg
    10321 cgccttatga ttgttttttc ctcgttacaa attcgtcgcg gcgtgcgcgt cctgctggat
    10381 aatgccaccg ccaccatcaa ccctgggcag aaagtcggcc tggtgggtaa aaacggctgt
    10441 ggtaaatcta ccctgctggc attgctgaaa aatgaaatca gcgccgacgg cggcagctac
    10501 acctttcctg gaagctggca actggcgtgg gtgaatcagg aaacaccggc gttaccgcaa
    10561 gcggcgctgg aatatgtcat tgacggcgac cgtgaatatc gtcaactgga agcgcagcta
    10621 cacgacgcca acgaacgtaa cgacgggcac gccattgcga ccattcatgg caagctggat
    10681 gctattgacg catggagtat tcgctcccgt gccgccagcc tgctgcacgg cctcggtttc
    10741 agcaatgaac aactggagcg cccggtaagt gatttctccg gtggctggcg tatgcgtctt
    10801 aaccttgccc aggcgctgat ttgccgttca gacttgctgc tgctcgacga accgactaac
    10861 cacctcgatc tcgatgccgt tatctggctg gaaaaatggc tgaagagcta tcagggcacg
    10921 ctgatcctga tctctcacga tcgcgatttc ctcgatccga tcgttgataa aattatttat
    10981 atcgaacaac aaagcatgtt cgagtacacc ggcaactaca gttcgtttga agtacagcgc
    11041 gccacccgtc tggcgcagca acaagcgatg tatgaaagcc agcaggaacg cgtagcgcat
    11101 ctgcaaagtt atatcgaccg tttccgtgcc aaagccacca aagcgaagca ggcccagagc
    11161 cgcattaaga tgcttgagcg tatggagctg attgcccccg cgcacgtcga caacccgttc
    11221 cgctttagct tccgcgcgcc ggaaagcctg ccaaatccgt tactgaagat ggaaaaagtc
    11281 agcgcaggct atggcgatcg cattattctc gactcgatta aactgaatct ggtccccggc
    11341 tcgcgcattg gtctgctagg ccgcaacggc gcgggtaaat cgacattaat caaactgtta
    11401 gccggggaac ttgcgccagt cagcggtgaa attggtctgg cgaaagggat caagctcggc
    11461 tacttcgccc agcatcaact ggaatacctg cgcgccgacg aatcgcctat tcaacatctg
    11521 gcacgtttag cgccgcagga gctggaacaa aaactgcgtg actacctcgg cggctttggt
    11581 ttccagggcg ataaagtaac cgaagaaacg cgccgcttct ccggtgggga aaaagcccgc
    11641 ctggtgctgg cattaattgt ctggcagcgt ccgaatctgc tgctgctcga cgaaccgacc
    11701 aaccaccttg acctcgacat gcgtcaggca ctcaccgaag cattaatcga gttcgaaggc
    11761 gcgctggttg tcgtctcgca cgaccgtcat ttgctgcgtt ccaccactga cgatctctac
    11821 ctggttcacg atcgtaaagt cgaaccgttc gacggcgatc tggaagatta tcaacagtgg
    11881 ttgagcgacg tacaaaagca ggaaaaccag accgacgaag cgccaaaaga gaacgcgaac
    11941 agcgcccaag cacgtaaaga tcagaagcgt cgggaagcgg agctgcgtgc gcaaacccag
    12001 ccactgcgta aagagattgc ccgtctggaa aaagagatgg agaagctgaa cgcgcaactg
    12061 gcgcaggcgg aagagaaact cggcgacagc gaactgtacg atcaaagccg taaagcggag
    12121 ttgaccgcct gcctgcaaca gcaagccagc gccaaatccg gcctggaaga gtgcgaaatg
    12181 gcatggctgg aagcccagga gcagcttgag cagatgctgc tggaaggcca aagcaactga
    12241 tggcgcagat aacgacgacc gatgccaatg aattcagcag cagtgctgaa ttcaccccca
    12301 tgcgcggctt tagcaattgt catctacaaa ccatgctgcc gcgtctgttc cgtcgcaagg
    12361 tgaaattcac cccgtactgg cagcggctgg agttgcccga cggcgatttt gtcgatctcg
    12421 cgtggagtga agatcctgca caggcgcggc ataaaccgcg tttagtggta tttcacgggc
    12481 tggaaggcag tctcaatagc ccttacgctc acggtctggt tgaggcggcg caaaagcgcg
    12541 gctggctggg cgtggtgatg cattttcgcg gatgcagcgg tgaaccaaac cgtatgcacc
    12601 gcatttacca ttcgggcgaa accgaagacg ccagttggtt tttacgctgg ctgcaacgcg
    12661 agtttggtca tgcgccaacg gctgccgtcg gctattcgct cggcggtaat atgctggcct
    12721 gtttgctggc aaaagaaggc aatgatctcc cgattgatgc ggcagtgatt gtctctgcgc
    12781 cgtttatgct ggaggcctgt agttaccata tggaaaaggg cttttcccgc gtttatcagc
    12841 gttacttgct gaacctgtta aaagccaatg ccgcgcgcaa gctggcagcc taccccggca
    12901 cactgccgat taatctcgcg cagttaaaat cggtacgtcg catccgtgaa tttgacgatc
    12961 tcatcaccgc cagaattcac ggctacgctg acgctatcga ctattatcgt cagtgtagcg
    13021 ccatgccgat gctcaatcgg atagccaaac cgacactgat tattcacgcc aaagacgatc
    13081 cgtttatgga tcatcaggtg atcccgaaac cggaaagtct ccccccgcag gtggagtatc
    13141 agctgactga acatggcggt catgttggct ttattggcgg tacattactt catccgcaaa
    13201 tgtggctgga gtcacgcatt cctgactggt taacaacgta tctggaggcg aaatcatgtt
    13261 gattccgtgg caagacctct cccccgaaac gctggaaaat ttaattgaaa gctttgtgtt
    13321 acgtgaaggc accgattatg gtgaacatga gcgtacactt gaacagaaag tcgccgacgt
    13381 caaacgccag ctacagtgcg gagaagcggt gctggtatgg tcggagctgc acgaaacggt
    13441 caatatcatg ccgcgcagtc agtttcgcga ataacctgca accgtcggcg gacgagataa
    13501 taatgatagc taagtcatgg agttactatg tctgccaaac atccggtcat tgcggtaaca
    13561 ggatccagcg gcgcggggac caccaccacc agcctcgcgt ttcgtaaaat attcgcgcag
    13621 ttaaatctgc atgcagccga ggtggaaggc gacagttttc accgttatac ccgcccggaa
    13681 atggacatgg cgatccgcaa agcgcgcgac gccgggcggc atatcagcta cttcggcccc
    13741 gaagcgaacg acttcggcct tctggaacaa accttcattg aatacggtca gagcggcaaa
    13801 gggaaatctc gcaaatatct gcatacctac gatgaagccg taccgtggaa tcaggtaccg
    13861 gggacattca ccccctggca atctttaccg gaacccactg atgtactgtt ttatgaaggt
    13921 ttacacggcg gcgtagtcac gccacagcat aacgttgcgc agcatgtgga cttactggtc
    13981 ggcgtggtgc ctatcgttaa ccttgagtgg attcaaaaac tgatccgtga caccagcgag
    14041 cgcgggcact cacgagaagc agtgatggac tcggtagtgc gttcaatgga agactatatc
    14101 aactacatca caccgcagtt ttcccgcacc catcttaact tccagcgcgt tcccaccgtc
    14161 gacacctcaa acccgttctc ggcaaaaggt atcccgtcac tggatgaaag ctttgtggtg
    14221 atccattttc gtaatctgga agggatcgat ttcccctggc tgctggcgat gttgcaaggc
    14281 tcattcattt cccacatcaa tacgttagtg gtgcctggcg gcaaaatggg tctggcaatg
    14341 gaattaatta tgctgccgct ggtgcaacga ttgatggaag gaaagaaaat cgagtaacgc
    14401 tgctattacg ccggataaaa tactatccgg cttcacaacg ggatagttaa atcacgcggc
    14461 aaccacttca tacgagtgag taatattcac cgctttttcc agcatcagcg ccactgagca
    14521 atatttctcg gcagaaagat caaccgcacg cgcaaccgct gcgtctttca gatcgcgacc
    14581 ggtgacgata aaatgtagat taatgtgcgt aaacaggcgt ggtgcctctt cgcggcgctc
    14641 agaggtcaat tttacttcac aatcgaccac atcctgacgc cctttttgca ggatagaaac
    14701 cacatcgatg gcactgcaac cacccgccgc catcaacacc atttccatcg ggcttggtgc
    14761 tttatcgcct gagttgccgt ccattaaaat ctggtgacca gaggcggatt cgcccagaaa
    14821 agttaaccct tcgacccact tcactcgcgc ttgcattttt gctactccac tgcgtcaatt
    14881 ttcctgacag agtacgcgta ctaaccaaat cgcgcaacgg aaggcgacct gggtcatgct
    14941 gaagcgagac accaggagac acaaagcgaa agctatgcta aaacagtcag gatgctacag
    15001 taatatattg atgtactgca tgtatgcaaa ggacgccaca ttaccgtgca gtacagttga
    15061 tagccccttc ccaggtagcg ggaagcatat ttcggcaatc cagagacagc ggcgttatct
    15121 ggctctggag aaagcttata acagaggata accgcgcatg gtgcttggca aaccgcaaac
    15181 agacccgact ctcgaatggt tcttgtctca ttgccacatt cataagtacc catccaagag
    15241 cacgcttatt caccagggtg aaaaagcgga aacgctgtac tacatcgtta aaggctctgt
    15301 ggcagtgctg atcaaagacg aagagggtaa agaaatgatc ctctcctatc tgaatcaggg
    15361 tgattttatt ggcgaactgg gcctgtttga agagggccag gaacgtagcg catgggtacg
    15421 tgcgaaaacc gcctgtgaag tggctgaaat ttcgtacaaa aaatttcgcc aattgattca
    15481 ggtaaacccg gacattctga tgcgtctgtc tgcacagatg gcgcgtcgtc tgcaagtcac
    15541 ttcagagaaa gtaggcaacc tggcgttcct cgacgtgacg ggccgcattg cacagactct
    15601 gctgaatctg gcaaaacaac cggatgctat gactcacccg gacggtatgc aaatcaaaat
    15661 tacccgtcag gaaatcggtc agattgtcgg ctgttctcgt gaaaccgtgg gacgcattct
    15721 gaagatgctg gaagatcaga acctgatctc cgcacacggt aaaaccatcg tcgtttacgg
    15781 cactcgttaa tcccgtcaga gtggcgcgtt acctggtagc gcgccatttt gtttcccccg
    15841 atgtggcgca gactgattta tcaccccgat atcaactatg cacttcgaca aacgctggtg
    15901 ctatgtttgc ccgtggccgt tgggttaatg cttggcgaat tacgattcgg cctgctcttc
    15961 tccctcgttc ctgcctgttg caatattgcg ggccttgata cgcctcataa acgttttttc
    16021 aaacgcttaa tcattggtgc gtcgctgttt gccacctgta gcttgctgac acaggtgcta
    16081 ctggcaaaag atgttcccct gccctttttg ctgaccggat taacgctggt acttggcgtc
    16141 actgctgagc tggggccatt gcacgcaaaa ttgcttcccg catcgctgct cgccgccatt
    16201 tttaccctca gtctggcggg atacatgccg gtctgggaac cgttgctcat ctatgcgctg
    16261 ggcactttgt ggtacggatt gtttaactgg ttttggttct ggatctggcg cgaacaaccg
    16321 ctacgcgagt cactaagtct gctgtaccgt gaactggcag attactgtga agccaaatac
    16381 agcctgctta cccagcacac cgaccctgaa aaagcgctgc cgccgctgct ggtgcgccag
    16441 caaaaagcgg tcgatttaat tacccagtgc tatcagcaaa tgcatatgct ttccgcgcaa
    16501 aataataccg attacaagcg gatgctgcgt attttccagg aggcgctgga tttgcaggaa
    16561 catatttcgg tcagtttgca tcagccggaa gaggtgcaaa agctggtcga gcgtagccat
    16621 gcggaagaag ttatccgctg gaatgcgcaa accgtcgccg ctcgcctgcg cgtgctggct
    16681 gatgacattc tttaccatcg cctgccaacg cgttttacga tggaaaagca aattggcgca
    16741 ctggaaaaaa tcgcccgcca acacccggat aatccggttg ggcaattctg ctactggcat
    16801 ttcagccgca ttgctcgtgt gctgcgcacc caaaaaccgc tctatgcccg tgacttactg
    16861 gccgataaac agcggcgaat gccattactt ccggcgctga aaagttatct gtcactaaag
    16921 tctccagcgc tacgcaatgc cggacgactc agtgtgatgt taagcgttgc cagcctgatg
    16981 ggcaccgcgc ttcatctgcc gaagtcgtac tggatcctga tgacagtatt gctggtgacg
    17041 caaaatggct atggcgcaac ccgtctgagg attgtaaatc gctccgtggg aaccgtggtc
    17101 gggttaatca ttgcaggcgt gacgctacac tttaaaattc ccgaaggtta caccctgacc
    17161 ttgatgctga ttaccaccct cgccagctac ctgatattgc gcaaaaacta cggctgggcg
    17221 acggtcggtt ttactattac cgcagtgtat accctgcaac tgttgtggct gaacggtgag
    17281 cagtacatcc ttccgcgtct tatcgatacc attattggtt gtttaattgc tttcggcggt
    17341 actgtctggc tgtggccgca gtggcagagc gggttattgc gtaagaacgc ccatgacgct
    17401 ttagaagcct atcaggacgc gattcgcttg attctgagcg agtacccgca acctacgcca
    17461 ctggcctggc agcgaatgcg ggtgaatcag gcgcataaca ctctgtataa ctcattgaat
    17521 caggcgatgc aggaaccggc gtttaacagc cattatctgg cagatatgaa actgtgggta
    17581 acgcacagcc agtttattgt tgagcatatt aatgccatga ccacgctggc gcgggaacac
    17641 cgggcattgc caccggaact ggcacaagag tatttacagt cttgtgaaat cgccattcag
    17701 cgttgtcagc agcgactgga gtatgacgaa ccgggtagtt ctggcgatgc caatatcatg
    17761 gatgcgccgg agatgcagcc gcacgaaggc gcggcaggta cgctggagca gcatttacaa
    17821 cgggttattg gtcatctgaa caccatgcac accatttcgt cgatggcatg gcggcagcga
    17881 ccgcatcacg ggatttggct gagtcgcaag ttgcgggatt cgaaggcgta atacaggcta
    17941 aaccatcgga tgaggtgttc acgccgcatc cgacatcttt tgcctgatgc gtcttattta
    18001 cgccccaatc accttcgcca ccgcgtgggc gaaacgatgc atcccttcat cgatatccgc
    18061 atcttccacc accagcgacg gcgcaaaacg catcacatca ggcccggcat tcagcaccat
    18121 tacgccttcc tctgcaccgg catacaggaa atcacgcgcc cgacctttgt actgtggttt
    18181 cagctctgcg ccaatcaaca gccccatacc gcgaatatcg ctaaacacat cgtactgctg
    18241 atcgatcttc tgcaaatggt caacaaaacg ctggcgtttc gcctgaatac cttccagcac
    18301 ttcaggggta ttgatgatat caaacgccgc gcccgctact gcacaggcca gaggattacc
    18361 gccgtaggtg gaaccgtgag aaccaggatg aaacgcggaa gcaatctccg ccgtggtcag
    18421 catggcgcta accgggaagc cgccacctag cgctttcgca gaggtcagaa tatccggcgt
    18481 cacgccgtag tgcatataag caaacagatc gccggtccgc cccatcccgc actgcacttc
    18541 atcaaacacc aataatgcct gatgctgatc acacagctcg cgcaagccct gcaaaaactc
    18601 tggcgtcgct gccgtcacgc cgccctcgcc ctgaatcggc tcaaccacca ccgcacaggt
    18661 atggtcatcc atcacggctt ttactgcgtg aagatcgtta aaaggcacgt ggatgatgtc
    18721 tgccggtttt ggcccaaagc cgtcggaata ttttggctgc ccacccaccg aaacggtaaa
    18781 cagcgagcga ccatgaaaag cgttatggaa ggcaataatt ttggttttga acgggctatg
    18841 acgcacacag gcgtaatggc gtgccagttt aaaggcggtt tcgttagctt ccgtgccgga
    18901 gttcataaac accacgcgtt cggcaaacgt tgcctcaatc agtttacggc caagacgcag
    18961 cgccggctca ttggtgaaaa cgttactaat atgccacaga gtttcgccct gggtttttaa
    19021 cgcgttcacc agcgcaggat ggcaatggcc caacgccgta actgcaatgc cacccgcgaa
    19081 atcgacatac tccttgccct gttgatccca gattcggctg ccctgacctt ttaccggaat
    19141 aaactctgcc ggagcataaa tcggcagaat cacttcatcg aaagtcgcgc gtgtaattgc
    19201 tgtttgttca attgccatct catgatcacc ctgttacgca taaataaata tgaaattata
    19261 atcacaaaat atgcataaaa aatcactgaa tggcaatcag aaatcagcga tgcagaaaat
    19321 tagccagcag ttgatgtcct tgttcgctaa gaatactttc tggatggaat tgcacacctt
    19381 ccagatccca ctggcgatgg cgaatcccca taatctcgcg ggtttcgctc caggccgtca
    19441 cttcaaagca ctccggtaat gagtcaggtt ccaccaccag cgaatggtag cgtgtcacgg
    19501 tgagtggatt tgccagcccc ttaaatacgc ccacaccatt atgtgtaatc ggcgaggttt
    19561 tgccgtgcat gacctttgcg gcgcgcacaa ctttgccgcc aaatgcctgc gccattgcct
    19621 gatgaccgag gcagacgcca agaatcggca agcgtccggc atagtgacga ataacgtcaa
    19681 gggagatccc ggcttcatct ggcgtacagg ggccgggtga gatgacaatt ttttgtggtt
    19741 taagggcgtc gatatccgcc aacgtcaacg catcgttgcg cttaaccaac acatccgccc
    19801 ccagttcaca aaagtactgg tagaggttcc aggtaaaaga atcgtagtta tcaataagca
    19861 ggatcatggc ggctccggta cgaaagaacc gcgctatttt actcagattc cccggcttcg
    19921 cttaccactt tgctaaatat catctgcagc gcggtgagat cccccattgc cccattctga
    19981 tttgcctgat tccaggcctc tttttcaata ccctgccagc tcagttgata tccggcatga
    20041 atcgccagtt gctcgaagaa gatccgctgt gccaaaccac ttcccacccg gaagggatgc
    20101 agcacgttga tttcacaata gtaatgcgcc agccgctcga cgaacttcgc tttctccagg
    20161 ccaaccagat aaccttcttc ctccagatcc tgcatcaggg cattgccctc tttttcgata
    20221 taagcaaagt ggcagaacgg agtatcgccc tgataaatat cgacttcgcg cagttgcccc
    20281 gcccagtcga aaatatcctg atacaactgg cgatgaagag tacgcaaatg cggtaaaccc
    20341 cgaaccaacg ggccaagttc aatggtcgca gcacgcagcg ccgtcatttc gtaagcggcc
    20401 tgttccagcc gctgctgctg gcggatgttc agccggttac gcatgatatc aaggcctgga
    20461 taaagatacg gatcgcgccc ttcgccgaat ttatcgctca tagtgcctcc tcagcgcttc
    20521 aagacgcgct aaagcctctg cggcagtaag agtgactaaa ggcatctcga cgccttcaag
    20581 acggcgactg gcctggaaat tacgattacg ctgaagctcc cagagacggg acttttgctt
    20641 atcggtgagt ttcttcactt gatgcctccc tgaacgtgtc catttgccac aagtataagc
    20701 ggcaaatccg ggttacgccg ggagagcaga agcgcgggca agattgcccg cgcgagaaat
    20761 cattacggca ggactttagc ggaaaggata actaccggtt ttgacggcac attctggtac
    20821 ggaccaacgt catgagtcgg cacctgggaa atcttatcgg caacgtccat gcctttcacc
    20881 actttaccaa ataccgcgta accgaaatca cgctgaccgt ggtcaaggaa ggcgttatcg
    20941 gcaacgttga taaagaactg gctggtggcg ctgtctttgt cagcggtacg cgccatcgcg
    21001 atggtgccac gcgtgttgcg caggccgtta tcagcttcat ttttgattgg cgggtttggt
    21061 tttttctgct gcatctgctc ggtgaaaccg ccgccctgaa tcataaagcc aggaatgacg
    21121 cggtgaaagg tcgtgttgtt ataaaaacca ctgttcacat aatcgacaaa gttttgcaca
    21181 gacactggtg ctttttgttt atccagctcc agttcgatgt taccagctga ggttgtcaac
    21241 aatacgtgcg ggtccccttt cgctgccatt gctgcgggag aaagagcaga aagagcgaaa
    21301 acagcagcca tcgccgccag ggtcgatttg aacatgagat ttccttaaca aacagagcag
    21361 aaaaaaagca agtgaagaga ttctaaatag ccttcaagaa caaggccatc cctttaccta
    21421 attttacgta tctgaaatat atgtaacctg aggataactt tttattccgt gatcaaaatc
    21481 acctcttaaa atgcaattta gcaaccgatt gcaataaaac atttaatcag atcacaaaat
    21541 cgcctaaaat cgcccgctcg cggcatagcc cgctatgcca ttttattttt gaacgcaggc
    21601 caatcatgac taacagcaat cgcatcaagc tcacatggat tagctttctt tcctacgcac
    21661 tgaccggtgc gttggttatt gtcaccggga tggtgatggg aaatatcgcc gattatttca
    21721 atctgcctgt ttccagcatg agtaacacct tcaccttcct caatgccggc attttaatct
    21781 ctatcttcct caacgcctgg ctgatggaaa tcgtcccgtt gaaaacgcag ttacgttttg
    21841 gctttctcct gatggtgctg gcggttgccg gtttgatgtt cagccacagc ctggcgctgt
    21901 tctcggcggc gatgttcatt ctcggggtgg tcagcggcat caccatgtcg attggtacat
    21961 tcctggtaac acaaatgtat gaaggacgtc agcgcggttc acgcctgtta tttaccgact
    22021 ccttcttcag tatggccggg atgattttcc caatgatcgc cgcgtttctg ctggcacgta
    22081 gcattgagtg gtactgggtt tatgcctgca tcgggctggt gtatgttgct atttttattc
    22141 tgaccttcgg ctgtgaattc ccggcactgg gcaaacatgc gccaaaaaca gatgctccgg
    22201 tagagaaaga aaagtggggg atcggcgtac tgtttctctc tgttgcggcg ctgtgctaca
    22261 tcctcggtca gttaggtttt atctcctggg tgccagaata tgccaaaggt ctgggtatga
    22321 gcctgaatga cgctggcacg ctggtaagta acttctggat gtcgtacatg gtcggcatgt
    22381 gggcgttcag ctttattctt cgcttctttg atttgcaacg tatcctgacc gtactggctg
    22441 gtctggctgc gattctgatg tacgtcttta acaccggaac accggcatat atggcgtggt
    22501 caattctcgc cctgggcttc ttctccagcg cgatctatac caccatcatc accctgggtt
    22561 cacagcagac caaagtaccg tcgccaaaac tggttaactt tgtcctgacc tgcgggacta
    22621 tcggtactat gttgaccttt gtggttaccg gcccgattgt tgaacatagt ggtccgcagg
    22681 cggcgctgct gacggcaaac ggtctgtacg ctgtcgtctt tgtgatgtgc ttcctgttag
    22741 gtttcgtcag ccgtcaccgt cagcataaca ccctgacctc tcattaattg ttcataccgg
    22801 gcggcactat cgtcgtccgg ccttttcctc tcttactctg ctacgtacat ctatttctat
    22861 aaatccgttc aatttgtctg ttttttgcac aaacatgaaa tgtcagacaa ttccgtgact
    22921 taagaaaatt tatacaaatc agcgatatac ctcttaagga gtatataaag gtgaatttga
    22981 tttacatcaa taagcggggt tgctgaatcg ttaaggtagg cggtaataga aaagaaatcg
    23041 aggcaaaaat gagcaaagtc agactcgcaa ttatcggtaa cggtatggtc ggccatcgct
    23101 ttatcgaaga tcttcttgat aaatctgatg cggccaactt tgatattacc gttttctgtg
    23161 aagaaccgcg catcgcttat gaccgcgtac acctctcgtc ttacttctct caccacaccg
    23221 ccgaagagct gtcgctggtg cgcgaaggct tctacgagaa acacggcatc aaagttctgg
    23281 tcggcgaacg cgctatcacc atcaaccgtc aggagaaggt gattcactcc agcgccggac
    23341 gtaccgtttt ttatgacaag ctgatcatgg cgaccggttc ctacccgtgg atcccgccaa
    23401 ttaaaggttc tgatactcag gactgctttg tctatcgcac cattgaagac ctcaacgcca
    23461 ttgaatcctg cgctcgtcgc agcaagcgcg gtgccgttgt tggtggcggc ctgttaggtc
    23521 tggaagctgc tggcgcgctg aaaaacttag gcattgaaac ccacgttatc gaatttgccc
    23581 ctatgctgat ggcggaacag cttgatcaga tgggcggcga gcagctgcgt cgcaaaatcg
    23641 aaagtatggg cgtgcgcgtt cacaccagca aaaacactct tgagattgtg caggaaggtg
    23701 ttgaagcgcg taaaaccatg cgttttgccg acggcagcga actggaagtc gactttatcg
    23761 tcttctctac cggtatccgt ccgcgcgata agctggcaac ccagtgtggt ctggacgttg
    23821 ctccgcgtgg gggtatcgtc ataaatgatt cctgccagac ttccgatccg gatatctacg
    23881 ccatcggtga atgcgcaagc tggaacaacc gtgtatttgg tctggtagca ccaggctaca
    23941 aaatggcgca ggtcgccgtt gaccatattc ttggtagcga aaacgccttt gaaggtgcag
    24001 accttagcgc caagctgaaa ctgctgggcg tagacgtagg cggtattggt gatgcgcacg
    24061 gtcgcacgcc tggcgcacgt agctacgttt acctcgacga aagcaaagag atctacaaac
    24121 gcctgattgt cagcgaagac aacaaaaccc tgctcggtgc ggtactggtg ggcgatacca
    24181 gcgactacgg caacctgctg caactggtgc tgaacgctat cgagctgccg gaaaacccgg
    24241 attccctgat cctgccagcg cactcgggta gcggtaagcc gtctatcggt gttgataaac
    24301 tgccggacag cgcgcaaatc tgctcctgct tcgacgtcac caaaggcgac ctgattgctg
    24361 ctatcaacaa aggctgccac accgttgcgg cgctgaaagc cgaaaccaaa gccggtacgg
    24421 gctgcggtgg ctgtatcccg ctagtcactc aggtactgaa cgcggaactg gcgaaacagg
    24481 gcatcgaagt taacaacaac ctgtgcgaac actttgctta ttcgcgtcag gaactgttcc
    24541 atttgatccg cgttgaaggc attaaaacct tcgaagaact gctggcgaaa cacggcaaag
    24601 gttacggttg tgaagtttgt aaaccgaccg tcggttcgct gctggcctcc tgctggaacg
    24661 aatacattct gaagccggaa catactccgc tgcaggattc taacgacaac ttcctcgcga
    24721 acatccagaa agacggcacc tactcggtga tcccgcgttc tccaggcggt gaaatcaccc
    24781 cggaagggct gatggcagtg ggtcgtatcg cgcgtgaatt taatctctac accaaaatca
    24841 ccggctccca gcgcctagcg atgtttggcg cacagaaaga cgatctgcca gagatctggc
    24901 gtcaactgat tgaagccggc ttcgaaaccg gtcatgccta tgcgaaagca ctgcgtatgg
    24961 cgaaaacctg cgtgggaagc acctggtgcc gctacggtgt tggcgacagc gtcggcctcg
    25021 gcgtggaact ggaaaaccgt tacaaaggca tccgtacgcc gcacaaaatg aagttcggtg
    25081 tctccggctg tacccgtgaa tgttcagaag ctcagggtaa agacgtgggt attatcgcca
    25141 ccgaaaaagg ctggaacctg tacgtttgcg gtaacggcgg catgaaaccg cgtcatgcgg
    25201 atctgctggc agcggatatc gatcgcgaaa cgctgatcaa atatctcgac cgcttcatga
    25261 tgttctacat ccgtactgcc gacaaactga cgcgtaccgc gccgtggtta gaaaacctcg
    25321 aaggtggcat cgattacctg aaagcggtga tcattgacga caaactgggg ctgaacgctc
    25381 atctggaaga agagatggcg cgcctgcggg aagcggtagt gtgcgaatgg actgaaacgg
    25441 tcaatacgcc gtctgcgcag actcgcttca aacacttcat caacagcgac aagcgtgacc
    25501 cgaacgtgca gatggtgcca gagcgcgaac agcaccgtcc ggcaacgccg tatgaacgta
    25561 tcccggtaac tctggtggag gacaacgcat gagccagtgg aaagacatct gcaaaatcga
    25621 tgacatcctg cctgaaaccg gcgtctgcgc gctgttaggt gacgaacaag tagcaatttt
    25681 ccgcccgtat cacagcgatc aggtgtttgc gatcagcaac atcgacccgt tcttcgagtc
    25741 cagcgtgctg tcacgcgggt tgattgcgga acaccagggc gagctgtggg tcgccagtcc
    25801 gctgaaaaag cagcgttttc gcttaagcga cggcttgtgc atggaagacg aacagttttc
    25861 cgtcaagcat tacgacgcgc gagtgaaaga cggcgtggta cagctgcgcg gttaatattt
    25921 taacgggagg cgcaatgcct cccctttttg catggtcctg taataacctt cggtatattg
    25981 caggacattt tttaaacttt ttgttttatt ttttgttttt attttttaaa ggataatcaa
    26041 atgtttacag acactattac taagtgtgcg gctaacgctg cgcgcattgc acgcctgtcg
    26101 gcaaataacc cgctcggctt ttgggtcagc tccgccatgg cgggcgcgta tgtgggtctt
    26161 gggatcatcc tgattttcac gctcggtaat ttgctcgacc cgtccgtacg tcctctggtg
    26221 atgggcgcga cctttggtat cgccttaacg ctggtgatta tcgccggttc tgaattgttc
    26281 accggacaca ccatgttcct cacctttggg gtgaaagcgg gcaccatcag ccacgggcaa
    26341 atgtgggcaa tcctgccgca aacctggctg ggtaacctgg tcggttccgt cttcgttgcc
    26401 atgctttata gctggggcgg cggtagcctg ctgccggtag ataccagcat cgttcactcc
    26461 gtcgcgctgg ctaaaaccac cgcaccggca atggtattat tcttcaaagg cgcattgtgt
    26521 aactggctgg tttgtctggc tatctggatg gcattgcgta cagaaggcgc ggcgaaattt
    26581 atcgcaatct ggtggtgtct gctggcattt atcgcgtccg gctacgagca ctccatcgct
    26641 aacatgacgc tgttcgcact ctcctggttc ggcaaccaca gcgaagccta cacgctggcg
    26701 ggcattggtc ataacctgct gtgggtgacg ctgggtaata ctttatcggg tgccgtattc
    26761 atgggattgg gttattggta tgctacgccg aaagcgaatc gtccggttgc ggacaaattt
    26821 aatcaaactg aaacggctgc cggttaatta ttaaggggtt tttacgtgga tcatttgcct
    26881 atattttgcc aattacgcga tcgcgactgt ctgattgtcg gcggtggtga tgtcgcggaa
    26941 cgcaaagcaa ggttgctgtt agacgcaggc gctcgcttaa cggtgaatgc attagcgttt
    27001 attccacagt tcaccgcatg ggcagatgca ggcatgttaa ccctcgtcga agggccattt
    27061 gatgaaagcc ttctcgacac ctgctggctg gcgattgcag cgacggatga tgacgcgctt
    27121 aaccagcgcg tcagcgaagc cgctgaagct cgtcgcatct tctgtaacgt ggtcgatgcg
    27181 ccgaaagccg ccagctttat tatgccgtcg attattgacc gctcaccgct catggtagcg
    27241 gtatcctctg gcggcacctc tccggttctg gcgcgcctgt tgcgcgaaaa actcgaatca
    27301 ctgctgccgt tacatctggg ccaggtagcg aaatacgccg ggcaattacg cgggcgagta
    27361 aaacagcagt tcgccacgat gggtgagcgt cgccgtttct gggagaaatt gttcgttaac
    27421 gaccgcctgg cgcagtcgct ggccaacaac gatcagaaag ccattactga aaccaccgaa
    27481 cagttaatca atgaaccgct cgaccatcgc ggtgaagtgg tgctggttgg tgcaggtccg
    27541 ggcgatgccg ggctgctgac actgaaaggt ctgcaacaaa ttcagcaggc agatgtggtg
    27601 gtctacgacc gtctggtttc tgacgatatt atgaatctgg tacgccgcga tgctgatcgc
    27661 gttttcgtcg gcaaacgcgc gggataccac tgcgtaccgc aggaagagat taaccagatc
    27721 ttgctgcggg aagcgcaaaa aggtaaacgc gtggtgcgtc tgaaaggcgg cgatccgttt
    27781 attttcgggc gtggcggaga agagctggaa acactgtgca acgcgggtat tccgttctcg
    27841 gtggttccgg gtattaccgc agcttctggt tgctctgcct attcgggtat tccgctcacg
    27901 catcgcgatt atgcccagag cgtacgctta attaccggac acttaaaaac cggtggcgag
    27961 ctggactggg aaaacctggc ggcagaaaaa cagacgctgg tgttctatat ggggctgaat
    28021 caggctgcaa ctattcagca aaagctgatt gaatacggta tgcctggtga aatgccggtg
    28081 gcaattgtcg aaaacggaac ggcagtcacg cagcgcgtga ttgacggtac gctcacgcag
    28141 ctgggcgaac tggcgcagca aatgaacagt ccatcgctaa ttattattgg tcgggttgtt
    28201 ggcctgcgcg ataaattgaa ctggttctct aaccattaat ttaaccaggg ccagagaagt
    28261 ctggcctttt tcgtttacaa ctctctttat atattcagca gataaaaaca taacccctta
    28321 ataaatattg tctgctaatg ggttatattc accgttacag cgacttacaa aattaagtca
    28381 atcgcttact gacgctaatt gaatattcct gtatcgttct taatg
//
LOCUS       DAZFFE010000056        28415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFFE010000056 DAZFFE010000000
VERSION     DAZFFE010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.056"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..469
                     /locus_tag="ACHJ69_002604"
     CDS             <1..469
                     /locus_tag="ACHJ69_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015563.1"
                     /translation="MLFLAVLFTGFNFNAEAATVKQALSCNPEAWAEQPGACPSTYEL
                     YEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNINGTVWFQGDGCKANTC
                     GWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVKNYVAAVN"
     gene            485..895
                     /locus_tag="ACHJ69_002605"
     CDS             485..895
                     /locus_tag="ACHJ69_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015564.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVAQDKLKI"
     gene            1116..1937
                     /locus_tag="ACHJ69_002606"
     CDS             1116..1937
                     /locus_tag="ACHJ69_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5015565.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     QLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            2019..2498
                     /locus_tag="ACHJ69_002607"
     CDS             2019..2498
                     /locus_tag="ACHJ69_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5015566.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVALEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            2514..2990
                     /gene="yeeS"
                     /locus_tag="ACHJ69_002608"
     CDS             2514..2990
                     /gene="yeeS"
                     /locus_tag="ACHJ69_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO5015567.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     KWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            3053..3274
                     /gene="yeeT"
                     /locus_tag="ACHJ69_002609"
     CDS             3053..3274
                     /gene="yeeT"
                     /locus_tag="ACHJ69_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO5015568.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            3348..3716
                     /gene="cbeA"
                     /locus_tag="ACHJ69_002610"
     CDS             3348..3716
                     /gene="cbeA"
                     /locus_tag="ACHJ69_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFO5015569.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            3805..4178
                     /gene="cbtA"
                     /locus_tag="ACHJ69_002611"
                     /pseudo
     CDS             3805..4178
                     /gene="cbtA"
                     /locus_tag="ACHJ69_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            4175..4369
                     /gene="yeeW"
                     /locus_tag="ACHJ69_002612"
     CDS             4175..4369
                     /gene="yeeW"
                     /locus_tag="ACHJ69_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO5015570.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAEKGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            4415..4495
                     /locus_tag="ACHJ69_002613"
                     /pseudo
     CDS             4415..4495
                     /locus_tag="ACHJ69_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4784..4930)
                     /locus_tag="ACHJ69_002614"
                     /pseudo
     CDS             complement(4784..4930)
                     /locus_tag="ACHJ69_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            4984..5166
                     /locus_tag="ACHJ69_002615"
     CDS             4984..5166
                     /locus_tag="ACHJ69_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO5015571.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(5267..5596)
                     /gene="tmaR"
                     /locus_tag="ACHJ69_002616"
     CDS             complement(5267..5596)
                     /gene="tmaR"
                     /locus_tag="ACHJ69_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO5015572.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(5768..6826)
                     /gene="yeeA"
                     /locus_tag="ACHJ69_002617"
     CDS             complement(5768..6826)
                     /gene="yeeA"
                     /locus_tag="ACHJ69_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO5015573.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(7025..7498)
                     /gene="sbmC"
                     /locus_tag="ACHJ69_002618"
     CDS             complement(7025..7498)
                     /gene="sbmC"
                     /locus_tag="ACHJ69_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO5015574.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(7617..8783)
                     /gene="dacD"
                     /locus_tag="ACHJ69_002619"
     CDS             complement(7617..8783)
                     /gene="dacD"
                     /locus_tag="ACHJ69_002619"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO5015575.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            8972..10399
                     /gene="sbcB"
                     /locus_tag="ACHJ69_002620"
     CDS             8972..10399
                     /gene="sbcB"
                     /locus_tag="ACHJ69_002620"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO5015576.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     RILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(10442..10669)
                     /gene="tsuB"
                     /locus_tag="ACHJ69_002621"
     CDS             complement(10442..10669)
                     /gene="tsuB"
                     /locus_tag="ACHJ69_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO5015577.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(10683..11741)
                     /gene="tsuA"
                     /locus_tag="ACHJ69_002622"
     CDS             complement(10683..11741)
                     /gene="tsuA"
                     /locus_tag="ACHJ69_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO5015578.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVTVL
                     LLITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYARPQRKARLATAAAN"
     gene            complement(11920..13278)
                     /gene="plaP"
                     /locus_tag="ACHJ69_002623"
     CDS             complement(11920..13278)
                     /gene="plaP"
                     /locus_tag="ACHJ69_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO5015579.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(13268..13330)
                     /gene="yoeI"
                     /locus_tag="ACHJ69_002624"
     CDS             complement(13268..13330)
                     /gene="yoeI"
                     /locus_tag="ACHJ69_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO5015580.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(13545..14473)
                     /gene="yeeY"
                     /locus_tag="ACHJ69_002625"
                     /pseudo
     CDS             complement(13545..14473)
                     /gene="yeeY"
                     /locus_tag="ACHJ69_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(14519..15343)
                     /gene="yeeZ"
                     /locus_tag="ACHJ69_002626"
     CDS             complement(14519..15343)
                     /gene="yeeZ"
                     /locus_tag="ACHJ69_002626"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5015581.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(15426..15680)
                     /gene="yoeB"
                     /locus_tag="ACHJ69_002627"
     CDS             complement(15426..15680)
                     /gene="yoeB"
                     /locus_tag="ACHJ69_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFO5015582.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(15677..15928)
                     /gene="yefM"
                     /locus_tag="ACHJ69_002628"
     CDS             complement(15677..15928)
                     /gene="yefM"
                     /locus_tag="ACHJ69_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFO5015583.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            16211..16261
                     /gene="hisL"
                     /locus_tag="ACHJ69_002629"
     CDS             16211..16261
                     /gene="hisL"
                     /locus_tag="ACHJ69_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO5015584.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    16237..16360
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            16407..17306
                     /gene="hisG"
                     /locus_tag="ACHJ69_002630"
     CDS             16407..17306
                     /gene="hisG"
                     /locus_tag="ACHJ69_002630"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO5015585.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLISMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            17312..18616
                     /gene="hisD"
                     /locus_tag="ACHJ69_002631"
     CDS             17312..18616
                     /gene="hisD"
                     /locus_tag="ACHJ69_002631"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO5015586.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASGRLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCNKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDTD
                     MAHQVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            18613..19683
                     /gene="hisC"
                     /locus_tag="ACHJ69_002632"
     CDS             18613..19683
                     /gene="hisC"
                     /locus_tag="ACHJ69_002632"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO5015587.1"
                     /translation="MNTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPIVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            19683..20750
                     /gene="hisB"
                     /locus_tag="ACHJ69_002633"
     CDS             19683..20750
                     /gene="hisB"
                     /locus_tag="ACHJ69_002633"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO5015588.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQ
                     KAGYKLVMITNQDGLGTQSFPQVDFDGPHNLMMQIFTSQGVQFDEVLICPHLPTDECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            20750..21340
                     /gene="hisH"
                     /locus_tag="ACHJ69_002634"
     CDS             20750..21340
                     /gene="hisH"
                     /locus_tag="ACHJ69_002634"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO5015589.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESTGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            21340..22077
                     /gene="hisA"
                     /locus_tag="ACHJ69_002635"
     CDS             21340..22077
                     /gene="hisA"
                     /locus_tag="ACHJ69_002635"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO5015590.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            22059..22835
                     /gene="hisF"
                     /locus_tag="ACHJ69_002636"
     CDS             22059..22835
                     /gene="hisF"
                     /locus_tag="ACHJ69_002636"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO5015591.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            22829..23440
                     /gene="hisIE"
                     /locus_tag="ACHJ69_002637"
     CDS             22829..23440
                     /gene="hisIE"
                     /locus_tag="ACHJ69_002637"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO5015592.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(23537..24514)
                     /gene="wzzB"
                     /locus_tag="ACHJ69_002638"
     CDS             complement(23537..24514)
                     /gene="wzzB"
                     /locus_tag="ACHJ69_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO5015593.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDEKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(24660..25826)
                     /gene="ugd"
                     /locus_tag="ACHJ69_002639"
     CDS             complement(24660..25826)
                     /gene="ugd"
                     /locus_tag="ACHJ69_002639"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO5015594.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVLK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELRDVADKVYTRDLFGSD"
     gene            complement(26071..27477)
                     /gene="gndA"
                     /locus_tag="ACHJ69_002640"
     CDS             complement(26071..27477)
                     /gene="gndA"
                     /locus_tag="ACHJ69_002640"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO5015595.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSVFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKGEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(27596..>28415)
                     /gene="galE"
                     /locus_tag="ACHJ69_002641"
     CDS             complement(27596..>28415)
                     /gene="galE"
                     /locus_tag="ACHJ69_002641"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO5015596.1"
                     /translation="IFSNHDIHTVIHFAGSKSVGESISKPIHYYNNNVVASLVLINEM
                     MKRGIYNLIFSSSATVYGNSNTMPVTENAPIGMTTNPYGTSKLMIEKILDDVTRANSS
                     FRVTVLRYFNPVGAHFSGEIGEDPNGIPNNLMPYVCQVAIGKYKQVSVYGRDYPTKDG
                     TGVRDYIHVMDLAEGHVAALEHRNEGPNHKVYNLGTGKGYSVLDLLTTFERVTFRSVP
                     YVLSERRPGDIAECWSDPSKAYRELGWKAKRGLEEMVRDAWNWQQKNPNGYIKA"
BASE COUNT         7378 a         7285 c         6912 g         6840 t
ORIGIN      
        1 tatgttattt ctggcggttc tgttcacggg ctttaacttt aacgcagaag cggctaccgt
       61 aaaacaagct cttagttgta acccggaagc ctgggctgaa caacctggag cgtgtccatc
      121 aacgtacgag ttgtacgaag gtgacgctac ctacaaagcc gctatcgaca aagcattaaa
      181 accggtcgga ctgagcggca tgttcggtaa aggcggctat atggatggcc ctggtggagg
      241 tatcacgcca gtaaacatta acggtacagt ctggttccag ggcgacggtt gcaaagccaa
      301 tacctgcggc tgggacttta tcgtaacact ctataaccca aaaacccatg aagttgttgg
      361 ctaccgttac tttggtttag atgacccggc ctacctggtt tggttcggtg aaattggcgt
      421 gcatgaattc gcgtatctgg tgaaaaacta cgtagctgcg gttaactaat ttaaggagtc
      481 tatcatgaaa acttttatca aaactttact cgttgctgta actattctgt tctctgtctt
      541 cgctactgcg aaacaagtaa aactgccaaa taacatcaaa tacgttaata ctacagaagc
      601 gttttcctgt accgaaattg acggtatgaa ttgccagaca aagaatcagt tcaactataa
      661 agataacagc tatgttttcg tgcttgaacg tggtggtgcc tggtgctacg actacactgt
      721 ctcggtagtt aacctgaaaa ccgggaaagc acaaatgatt gaatacgggg acaatcaact
      781 gtgctcaggt agcaacaaac cgttcttcga aatcaaaaat ggcgtaccga cggtaggagt
      841 catcgacaca tccggaaaac ctgtcgttgt agcccaggac aaacttaaaa tctaaggagt
      901 aaaccatgcg tttctctgca cattattaat atcaaaggta aatacagccc cagccattgt
      961 taccggaaag ccgcttaatg cgtggctcgc cagtgaaagg cctctcagac aataaacctt
     1021 attcatttca cccgtcaggc cgcgtcttct ccgggagacg cggctttttt catttattgc
     1081 accagtaaat cttaaccacc gataaggagc aaagtatgcg attagcaagt cgttttggtt
     1141 atgctgcaaa ccagatacgc cgtgaccgtc cgctgacaca tgaagaactg atacgccatg
     1201 tacccagtat ttttggggaa gaccggcaca cctcccgcag tgaacggtat gcgtacattc
     1261 ccaccattac cgtcctggaa aatctgcagc gggaaggctt tcagccgttc ttcgcctgcc
     1321 agacccgtgt gcgcgacccg ggccgccggg gatacacaaa acacatgctg cgtctgcggc
     1381 gggccggaga gataaacgga gaacatgtcc ctgaaattat tcagctcaac tctcatgacg
     1441 gtacctccag ctaccagatg ctgccgggtt acttcaggtt cgtctgccag aacgggtgcg
     1501 tctgtggcca gtctctgggg gaagtgcgtg ttccacaccg gggaaatgta gtggagaaag
     1561 ttatcgaagg ggcttacgag gtggtgggcg tgtttgaccg gatagaggag aagcgtgatg
     1621 ccatgcagtc gctggtcctg ccgccaccgg cacgccaggc gctggcacag gcggcactga
     1681 cttaccgtta tggtgacgaa catcagcccg tcaccaccgc cgacattctg acgccgcgac
     1741 gccgggagga ttacggtaag gacctgtgga gcgcatatca gaccatccag gagaatatgc
     1801 tgaaaggcgg gatttccggt cgcagtgcaa aaggaaaacg tatccatacc cgggccattc
     1861 acagcattga taccgacatt aagctcaatc gcgcattgtg ggtgatggca gaaacgatgc
     1921 tggagagcct gcgctgatgc cgtttccctg agtgagggaa tcattcagct ttcgttcctg
     1981 aaaaacacac atttatttgt tatttaagga gttatctcat gaaaaccgtt tctcagaata
     2041 cccccacaat ttattcagct acaacaccag agaataatcc gcctcagttg gttgccagcc
     2101 tcgtccctga tgaacagcgc atcagcttct ggccgcagca ttttggcctc attccacagt
     2161 gggtggccct ggagccccgt gtcttcggct ggatggaccg tctgtgcgaa gactactgcg
     2221 gtggtatctg gaatctgtac accctgaaca acggcggggc atttatggca cccgaaccgg
     2281 atgacgatga tgacgaaaca tgggtactgt tcaatgccat gaacggtaac cgcgcagaaa
     2341 tgagcccgga agccgccggt attgccgcct gtctgatgac gtacagtcat catgcctgtc
     2401 gtacggagaa ttatgccatg acggtccatt attaccggtt gcgggattac gccctgcagc
     2461 atccggaatg cagcgccatt atgcgcatca tcgactgaat ggagagaagc acaatgcaac
     2521 aactttcctt tctgcccgga gagatgacgc ccggcgagcg cagcctcatt caacgggccc
     2581 tgaaaaccct ggaccgccat cttcatgaac ccggcgtggc cttcacctcc acccgtgcag
     2641 cacggaaatg gctgattctg aacatggcgg gactggagcg tgaagaattc cgggtgctgt
     2701 atctgaataa ccagaatcaa ctgattgccg gtgaaaccct ctttaccggc accatcaacc
     2761 gcacggaagt ccatccccgg gaagtgatta aacgcgccct gtaccacaat gccgctgccg
     2821 tggtgctggc acacaatcac ccctccggtg aagtcacacc cagtaaggca gaccgcctta
     2881 tcacggaacg tctggtacag gcactgggcc tggtggatat ccgggtgccg gaccatctga
     2941 tagtcggtgg caaccaggtt ttctcctttg ccgaacatgg tctgctttaa cccgtcacaa
     3001 ccacatcaca cctgttttca cttttatctt ctgtcttcag aggtatccca ttatgaaaat
     3061 tatcacccgt ggtgaagcca tgcgtattca ccaacaacat ccgacatccc gtctttttcc
     3121 gttctgtacc ggtaaatacc gctggcacgg cagtgctgaa gcgtataccg gtcgtgaagt
     3181 gcaggatatt cccggtgtgc tggccgtgtt tgctgaacgc cgtaaggaca gttttggtcc
     3241 gtatgtccgg ctgatgagcg tcaccctgaa ctgagtggga attctgatga gcagaattat
     3301 cgccactacc ggaccattct tagccgattt tctgtaagga ttttatcgtg tcagacacac
     3361 tccccgggac aacacttccc gacgacaatc acgaccgccc ctggtggggg ctgccctgca
     3421 ccgtgacgcc ctgtttcggg gcacgtctgg tgcaggaggg taaccggttg cattaccttg
     3481 ccgaccgcgc cggtatcaga ggcctgttca gcgatgcaga tgcgtaccac ctggaccagg
     3541 cctttccgct gctgatgaaa caactggaac tcatgctcac cagcggtgaa ctgaatcccc
     3601 gccatcagca taccgtcacg ctgtatgcaa aagggctgac ctgcaaagcc gataccctca
     3661 gcagttgtgg ttacgtttat ctggctgttt atccgacgcc cgaaatgaaa aattaactct
     3721 ccagaatagc cttctgctac ggcctggtgt tttcaccacg ccacttttcc atttttatat
     3781 ctgcatatca ggaaaatctt cagtatgaaa acattacctg tattacccgg gcaggcgacc
     3841 agttctcgcc cgtctcctgt tgaaatctgg cagatactgc tgtcccgact gctggaccag
     3901 cactatggcc tcacactgaa tgacacacct tttgccgatg aacgtgtgat tgggcagcat
     3961 attgaggcag gcatttcact gtgtgatgcg gtgaactttc tcgtggaaaa atacgcgctg
     4021 gtgctaccga ccagccggga ttcagcgact gtacccgctc tcagttaata aacagcatcg
     4081 atatcctccg ggctcgcagg gcgaccggcc tgatgacccg cgacaattac agaacggtaa
     4141 ataacattac cctgggtaag tatccggagg cgaaatgatg acactggaag ccgacagcgt
     4201 taacgtacag gcgctggata tggggcacat tgtcgttgac attgatggtg ttaatatcac
     4261 tgaactgatt aataaggccg ctgaaaaagg ttattcactc cgcgtggtgg atgaccgtga
     4321 ctctaccgaa acaccggcaa cttatgccag ccctcaccag ttgctgtaag acaatgcagt
     4381 gatgcagggt atagtgccat taaccacaga aacagcactg ttgtcctgta ttaagcaggc
     4441 aagagaagat gtccttaccc tgcgccatct gcagcttctg caccagaacc ggtgaatctc
     4501 actcaacagg cttcatttcc cctgacgcca gcctgaatac agctggcgtt ttcatttata
     4561 aacagaaagg aaaaccgata ataatggaat ggacgccccg accatgaagc aacggtagga
     4621 tattactgat aaaccttcca gtggaggtgc gttatggccg ttttaacgat tagcattgac
     4681 ctgacaaaaa atgaattcca aatccacggt ctgggtagga acagaaaaat ctaagctcag
     4741 aaaacggatt aaagtaagcg tcaacggagc accgtattga cgcttattta ttggtgagaa
     4801 ctacgttcca tggcaggagt tcgtcaacac ggttggaggg ccattccggc agtatgctca
     4861 gaatatggcg cagatacgct tccggatcga taccgttcag acggcaggtg cctgcttttc
     4921 cggcaacaag caggaatctc agcgctgaag caggaattag aggtaaaaac gccctaccgg
     4981 gccatgaatc acccggtcat tggagtagta accaaagcag atttagccag catggaacaa
     5041 atatctttgg tgaaaagttg gttacgggaa gctggagcgc acaacgtgtt agtaaccagt
     5101 gcagttaata acaatggagt tacagaactt tttgccttgc tgcatacaga agaaggctgt
     5161 tgttaatttc gattggttct cagatgagaa tgatgacggt gatacacaga taagaatcaa
     5221 tgagcaatga gggttgccgg gcaaccctca ttgaataaaa cgggaattac ttcgcttcgc
     5281 cgtttttcat ttcgcccata gctttcagct ttttggagat atcgcggcgt tctttggaga
     5341 gctcggcatt tttgatgatg taatcgtcaa cgcgatcttc atagtcacct ttcatgctgg
     5401 cgatgatgcc ctggattgct tcaacgctca tccctggctt gatgtaatcg ctcaggttgt
     5461 ccagcaacag gacgcgcttc tggttgtcac ggatcttttt ctcaacgtcc tgaatttcac
     5521 gttgcagttt gttcttacga cggaacagac gaacaaattc cagtacgtcc tggaatgaag
     5581 gcttggtagt ttccattttt atacccctga taatgtgaga gtcggattcg tttaagcaac
     5641 cgctattcgt tagggccaac attactcata gctgccgcag atgacaatgc ttttatccct
     5701 tttcactatc atacccttta tccttgctga atcgaagcag cagcaagatg attctgaagt
     5761 tcaggaatta tttgcgcaag gcccggcaaa tcagattcga tagcaactca agctgatgtg
     5821 ccgtctccat gttcagccac acataaccat agattggtgt ttccacaacc ttcaggtcat
     5881 ggtggttatt gagcaactga cgcagctctt ccacagcatc gttcaatttt tccgtattgg
     5941 caaaaaccgg ctgcggatta ccttcgtaca gcgcatgaac aaggctcagc agtatttgct
     6001 gcatcatatg ctgggtgtca cgaagttttt gcgcgttcaa taacacgaaa tggctggggc
     6061 gcgtggccca gtacgcattg atttgcaact ccagcataca aaccagattg cgattaatgg
     6121 tctggatacc ttcatatatc gattttggaa tacgggtttc tttgctggcg ggcgcaatca
     6181 gcccacgcat tttcacggca tcggtcagga gtttttgtag atggctttcc agacgtgggc
     6241 gttcgagtaa gttcggtgag aatgcagatt gataaacccg attatactcg gtcagacttt
     6301 tcgccagttg aatgcgccag tggatgaacg cccgttgtgg ccagataccg gtaaacaaca
     6361 ttgcgagtaa agagccgagg atcacatcgc cgcttcgcca taacgccgta tcaatttcac
     6421 ctgtcgggga accaacaaca attgccagcg tcaccccaat caataatcct tgatacggtt
     6481 tcttgcccag tgccagccaa ccgcaaagaa acatagccgc cgcgcaccag actaacatca
     6541 gcggtaacga aattaactcc agttgcagag cgataagacc taatatcgaa cccaacaccg
     6601 taccgccaat acgttcgaag gctcgaggga caacgttacc ccagaacgag atgggcccca
     6661 taatcaccac catggtgacc agcggccagg tactttccgg gatagtaaac aggcggataa
     6721 tgagaaaagt gagcaggaat gccagcgcga cccgagtacc atgcacaatg cggtaatggc
     6781 ggtatacccg gatttcaaac gggcttaatg acttatcggc acgcacgcag aaactccgta
     6841 atgaagatta aaagcgctca taactcatct gtcggccaga aggcgcgcct ctgaattatc
     6901 cgacggacaa accgggaaca gtgtaatttc ccggcaggag cgaaaaaatt gaaaggcgca
     6961 tcttactctt ttcgctttca aaaaaaagag tggtcatcgc gttaacacac cgccctgaga
     7021 tgaattagtg atgttttggc tgcaccgcaa catacatttc gatatcccag tacccatctt
     7081 ccgcgccatt gttcaaataa acctcgaagc agggctttgg taacatttca taagcactgt
     7141 cctgcaagag gctattaaag aactgatacc agggtttagc aaaatcatca ccgactacac
     7201 gagctaccgc caccgcatac tgaccacctg aaatttctgt cagaatgacg ccctcactgt
     7261 tttcggggag cgtaaagtaa cccggcaccg tcacgacggt gtcgcagcgt aatttttcgg
     7321 cgggtgtttc atctggattg tcgtaataga cagcaaccca ttccttcggc acaatatttt
     7381 tgctatctac ccacatcatc aactgctcaa aacctttctt taccgtctgt tcccacgggc
     7441 caacgagatg gaaacctgca acggtacgtt tatcttcctg cttaatctcg tagttcatga
     7501 cgcctccatt aatactgttt ttatatacag tatagttgca atattaaaac cacaaggaat
     7561 gagtgttgat tatgcgagca gactcgcact cctgccagtc tgctgcaaaa gaaaggtcag
     7621 gccttatggt ggaaataatc actaaggcga gaaaacatgc tgccttcccc gacagattcc
     7681 agggtaacca gcggccagtg cgccacctgt ttatcacggt cgtaaagttc aatttcccct
     7741 acccgctgat gggcgctaat tggcgcggtg agttctttac catcaagggt atatttggct
     7801 ttgatatgtg gaatttcggc tttcggtagt accatccaga actcttgttc tgttcccagg
     7861 gcgatatttt ctttatcgcc ataccatatg cgttccgtac cgaccttttt cccacggtgc
     7921 aaaatttgca ccgtagtaaa gttttgttgc ccccaacgca gtaattttct tgcctcttcc
     7981 tcacgacctt ttgcgctgtc agcacccatt accactgcaa tgagacgacg ctgcccatct
     8041 acagccgaag caatgagatt aaacccggca ccagaagtat gacccgtttt caggccgtca
     8101 acattcatgg ttttatccca caataacccg ttacggtttt gctgggtgat accgttccag
     8161 gtgagacttt tctcactgta catatgataa aactcgggct cgccgtggat gatagcacga
     8221 gaaagcacag ctaaatcata agccgagcta tgctggccag gtgcatccag accatgcact
     8281 gtttcaaaat gcgtatcctt gagatgcagc ttctcggcat agttgttcat catttcaaca
     8341 aactgccgtt gcccaccggc aatatagtca gccagagcaa cacaagcgtc atttccggaa
     8401 tccacaatta aaccacggct taaatcacgt accgatacgc gatcgccctc tttcaaaaac
     8461 atcagtgaag aaccgacaaa caccggatta tctttcgccc acgcatcgcg ccccacggtg
     8521 acaatatcgt ctggcgtaat gcgatgacta tcgatagcgc gatccacgac ataacccgtc
     8581 atcagctttg tcaggctggc gggattgcgc tgttgatgct cattacccgc ggtgaggatc
     8641 tgaccggtgg tgtaatccat caatacccag gacccggcat gaatctctgg aggctgaggt
     8701 gaaaaaggaa tgttttccgc cgcaaaacca gacgataagt taaaaacgaa caaagaagca
     8761 gcaataataa gacggcgttt caacagcaaa ccctcaggag tttcaaatag ctgttctttt
     8821 tacggaaata cttatgaact ggctggaata aagtgcaaga aaatgtgact accctctcat
     8881 ttttatctga catgatcttg tggcgctaaa gctgattagt acggtgatat ttgatactct
     8941 ggcagacagc agaaataacg gatttaacct aatgatgaat gacggtaagc aacaatctac
     9001 ctttttgttt cacgattacg aaacctttgg cacgcacccc gcgttagatc gccctgcaca
     9061 gttcgcagcc attcgcaccg atagcgaatt caatgtcatc ggcgaacccg aagtctttta
     9121 ctgcaagccc gcggatgact atttgcccca gcctggagca gtattaatta ccggtattac
     9181 cccgcaggaa gcacgggcga aaggagaaaa cgaagccgcg tttgccgccc gtattcactc
     9241 gctttttacc gtaccgaaga cctgtattct gggctacaac aatgtgcgtt tcgacgacga
     9301 agtcacacgc aacgtttttt atcgtaattt ctacgatcct tacgcctgga gctggcagca
     9361 tgataactcg cgctgggatt tgctggatgt tatgcgtgcc tgttatgccc tgcgcccgga
     9421 aggaataaac tggcctgaaa atgatgacgg tctaccgagc tttcgccttg agcatttaac
     9481 caaagcgaat ggtattgaac atagcaacgc ccacgatgcg atggctgatg tgtacgccac
     9541 tattgcgatg gcaaagctgg taaaaacgcg tcagccacgc ctgtttgatt atctctttac
     9601 ccatcgcaat aaacacaaac tgatggcgtt gattgatgtt ccgcagatga aacccctggt
     9661 tcacgtttcc ggaatgtttg gggcatggcg cggcaatacc agctgggtgg caccgctggc
     9721 gtggcatcct gaaaatcgca atgccgtaat tatggtggat ttggcaggag acatttcgcc
     9781 attactggaa ctggatagcg acacattgcg cgagcgttta tataccgcaa aaaccgatct
     9841 tggcgataac gccgccgttc cggttaagct ggtgcatatc aataaatgtc cggtgctggc
     9901 ccaggcgaat acgctacgcc cggaagatgc cgaccgactg ggaattaatc gtcagcattg
     9961 cctcgataac ctgagaattc tgcgtgaaaa tccgcaagtg cgcgaaaaag tggtggcgat
    10021 attcgcggaa gccgaaccgt ttacgccttc agataacgtg gatgcacagc tttataacgg
    10081 ctttttcagt gacgccgatc gtgcagcaat gaaaattgtg ctggaaaccg agccacgtaa
    10141 tttaccggca ctggatatca cttttgttga taaacgaatt gaaaaactgt tgttcaatta
    10201 tcgggcgcgc aacttcccgg ggacgctgga ttatgccgag cagcaacgct ggctggagca
    10261 ccgccgccag gtcttcacgc cagagttttt gcagggctat gctgatgaat tgcagatgct
    10321 ggcacaacaa tatgccgatg acaaagagaa agtggcgctg ttaaaagcgc tttggcagta
    10381 cgcggaagag attgtctaat ttgaagcctc cgccgctggt acggcggagg tcaatgttga
    10441 gtcaggcttt ttgaacggtg atgctccacg ctgcatcgcc aatctgctga taatcggtga
    10501 tggcatgccc ttcttctgcc gcccactgtg ggattgcttc agttgcctgg gtgcaatcaa
    10561 actcaattac caactcgtcg ccgctggcca tttctgccag tgctgctttt gcttctatga
    10621 gtggaaacgg acagacctgc gtcaccacat ccagcttttt aatcgccatc tttacacctt
    10681 acttaatttg ccgcagcagt tgccagtcgc gccttacgct gcggtcgagc atacacaagc
    10741 caggacgcag tccagactcc gagaatcata aatacaaggc caatccagcc ctgccaggtc
    10801 atcattgcag tcataactag tccattaccg atagagcaac cacctgcaat actggcaccg
    10861 aagcccatca gtacaccacc taacccgcta cgtaatgttg tttgtgcatc agctgcgcga
    10921 acgcggaact cacggctcgc tttggctgca ataaatgacc ccacgaagat ccctaacacc
    10981 agaaaaacgc cccagttaat gtatttcacg tcacccgcga ccagaaattg cagaatattg
    11041 gccgttgggg aagtgattcc taacccaaac atgcgtccgg ttgcttcact cagtggccag
    11101 gctaaaagcg caatcaaacc gatgagtaca gcagtgacaa agggatgcca gcgtttttca
    11161 aacagaatat gagcgatccc ggttcggcgc ggcggtaagg tcgcgacttt gagttttggc
    11221 ttcttcaact ctttcatcac cacccagagc gttatcaaca gcaaaacggt aaccaacggc
    11281 cacacagaca aattaaaagt ctcagcaata gagttatgtt cagtactgta gtgctgcaag
    11341 gtttgattta aaccactggc atgtggagaa cgcatcactg cactcatcac catataagtg
    11401 aaaagcgcga tccaactgcc gatcaatccc tcacccgcgc gataccaggt cccggtggca
    11461 catccacccg ccagaacaat tcccagcccg aagatatacc cacctataac agtaccaagc
    11521 cacgggaacg ccccggcttc gtaagtcagt aggcccgcct gaattaacgc aaaaaccccc
    11581 acgctttgta ccgaaattgc aatcagcaag gcgtaaaaca tgcgattatt tttcgcgata
    11641 tacatatcgc gaaaaccacc tgtcaggcaa aaacgcccac gctgcatgac aaatcccagc
    11701 agagcaccac aaattagccc gcttaatatc attgaaaaca taaattaatt aaccagatga
    11761 atgttaatga ggaaattatt catgactggt ggaatgcaga ccaataacca aattctctaa
    11821 attagaacaa atggttatta atgaggattt cgataaaaaa gaataaaaaa accggagcca
    11881 aatattcatt cgactccggt tgattattag aaaagatgat tatgctacgt cttcgtactg
    11941 cggaaccggg ttacggaagc tcttggtgac gcaagccagg taaatcagac cgatagcagc
    12001 ccagatcagc cccagaacca ttgagctttc ttccaggtta acccacagcg caccaacggt
    12061 cagcgcacca cacatcggca ggaacagata ctggaagtga tctttcagcg tcttattacg
    12121 cttctcacgg atccagaact gcgagatgac cgacaggtta acgaaggtga acgccaccaa
    12181 cgcaccaaag ttgatcagcg ccgtagccat caccaggtcg aagttgattg ccagcagggc
    12241 aatcgcgcca accaggatga tgttcatcgc cggagtacgc catttcgggt gtacataacc
    12301 gaagaagctt ttcgggaata cgccgtcacg acccattacg tacatcagac gcgctacgcc
    12361 tgcgtgcgcc gccataccgg acgccagtac ggtaatggtg gagaagatca gcgcgccaac
    12421 ctggaacgct ttacctgcaa cgtacagcat gatttcaggc tgtgacgcat ccggatcttt
    12481 aaagcgagag atatccggga agtacagctg caggaagtaa gttgcaaaga tgaagatcat
    12541 gccgccgatc agcgcggtca ggaaaatcgc acgcgggatc acgcgctctg catctttggt
    12601 ttcttccgac aggttgctga tgccgtcaaa gccggtaaag gagaagcaca ggattgtcgc
    12661 cccggtaatc atcgggataa catgcgcatc gccagaccag aatggacgag tgctcgccag
    12721 cgtaccagcg ccttcacctt caaatacgcc ataaacaacc atgcccagaa tcaccgcgat
    12781 cagcactacc tgcaacacga cgattacagt gttgaagttc gctacggatt tcagactacg
    12841 caggttaaag gcggtcataa aggccaccag cgccaccaca aacatccacg atgggatgga
    12901 aggcaccaga gcttcaaaat agattttcgc cagcagaatg ttgatcatcg gcgcgaacag
    12961 atagtcgagc agagaagacc aacccaccat aaagccgaca gtcgggctaa tggatttctg
    13021 ggcgtaagta tatgcagagc cagcagaagg atagcggcga accagcttcc cgtagctcag
    13081 agccgtaaac aggatcgcaa tcaatgcgaa cgcataggct gtcggcacat gaccatccgt
    13141 gaggcctgaa acgataccaa aagtatcaaa cagcgtcatc ggctgcatat aggcaagacc
    13201 catcattaca accggaacta acgtaagcgt tttacgcaat tccacgcgag aggtgtttgg
    13261 agtaacgtta tgcgacatgg tcattctcct ttacggtgat aaccgtcgcg taagcaaaaa
    13321 attgccccat ttttttggat tcctcagcga caacaactgt cgatttttag taaatatcta
    13381 tccggtacga agcccggcct cttggtatga atgattggtt tgaagcaaaa aataaccgac
    13441 gctgatgaaa cgtcggtttt tagtcatttt ttgacagcgg cgcattgtgc ctaaatgggg
    13501 gggaaatgac aagagaatga gaggcttgtc ataataattt ttctttaaat ggctgatttt
    13561 ccgtcatcag attttgcata aacaccggcg atcgcactat ttgctaaaat ttcatcccgc
    13621 caccagccgc tggcaagccc tgcggtctgt tcgttccagc caatccagac gggttcaaag
    13681 agagtttggg cgaccacttt cttctcgatt aacgcgccac tatcgagaaa acgttgtgcc
    13741 agataacggg gtaaatagcc gcagccgagg ccgctaattt gcagctccag cttggtttta
    13801 aaatcgaaaa cggtaatggc ttcttgttcg tcgagaagtt gtgaggccgt agaagcgcct
    13861 gcctgcgcgg tatcccccac cacaatcgcc cgatatcgct taataatacg acgatttaac
    13921 ggctcttctt ccatggctaa cggatgatga ggcgcgaccg caaagacttg ttccagatcg
    13981 cccaaccgcg agaaaccaaa ctcactggaa gatggtggct catgcatcgc gccaacaata
    14041 atatccgctc gcccctgagt cagggcatcc caggaaccac cgagtacgcc attgataaac
    14101 ttcaggcgcg tcacgctatg atgttgataa aaagcttcaa tcagtggcgc aagcagagaa
    14161 aaaggaaacg tgtcgtccac gccaatcacc agctcgtttt cccagccttc atgcagtttg
    14221 atcgcctgct tttcagttcc cgcacggtat gcagaacttc ccgccctttc tctaataaca
    14281 tttttccggt gcgggtgaat ttagcgcggt gaccgctacg atcaagcaat tgaatattaa
    14341 ggtcgctttc cagcttgtga acggtgtaac tcagagcgga cggcgtcttg tagagtttgg
    14401 ccgacgccgc cgcaaaactg ccttcttttt ctaaggcatc gaggatcatc aacacatcca
    14461 gcagtggttt catacacgcc cccttgcagt gcatgcggcg tcctgctggc gtgatcgttt
    14521 actccagcgg cattaccagc ggatcgggat actggtattc aaatcccagt tcattacaaa
    14581 tccgactgcc atcaataatc ttacctttgc cgctgtccag actatttctg aactgcggag
    14641 gttccagccc cagtaaacgg gccatctgcg gatagaaaac attacgcgca gggtgagctg
    14701 gcgcacatat attatagatg tgtccgcctt taggtgcctg taacaacaga gtgatagcgc
    14761 caataacatc ttctaaatgg actaaattaa caccatgttc accatcaggc gcggtttttc
    14821 cggcaaagaa ccgtccggga tgacgtcccg gtccgaccag gcccgcaaga cgcagaatat
    14881 cgaccgaagt accgggtaaa ttgtgcagcc agtcttcgag ttcttctaat actcgtccac
    14941 tgttggtcac cggattacgc ggggtggttt ctttcaccgt gccttgcgcg tcgccataga
    15001 cagacgttga gctggtaaaa ataatgcggg gaatacgatg ggccagcgcg ctatccacta
    15061 actcttgtac cgcttgtaaa tagaactcat cgccggggcc gctacgacgt gccggaagcg
    15121 taatgaccag cgcatcggca tccatcaggg catccagatc gtcagaatcg caaactaact
    15181 caggctccat gcgaagcaga tagctatcaa tgccactcat tcgggccgct tcgacaccat
    15241 cttgtgtggt tttactcccg gtgacttgcc agcctcgcgc tgaaagtgac atcgccagcg
    15301 gcatgcctaa ccaccctaac ccgacaattg cgaccttttt catccgttat ctcctgatac
    15361 accttagatc tataaggcta cgctagcgta tcaaaactga caattcattc tatgaatgaa
    15421 tctgttcaat aatgataacg acatgctgca atgagcagtg aatcgtcggt aaccgcgtat
    15481 accagacggt gctcctctgt aatgcgtcgg gaccagaaac ctgacaaatt atgtttcagg
    15541 ggttctggct tccccttacc ttcaaatggc gttctgcggg tatctttgat aagttcattg
    15601 atctttttaa caattcgctt atctgtttcc tgccagtaca gataatcatc ccatgattcc
    15661 tcagaccaga ttagtttcac tcaataatgt ccttttctgt tcctttgcct gatttcaggc
    15721 tatcgattga gtccatcaat ctccgggcgt tagcggggga acgcagtaga taagccgtct
    15781 cttccagcga gttgtattct tcgagtgaca tcagaacaca agcctctcca ttctgacgag
    15841 taataaggat cggggcatga tcttcaacgg ctttcatcat tgttgccgac aaattctgac
    15901 gcgcttcgct gtagctaatt gtacgcatgt caatctcctc ttttgtacag ttcattgtac
    15961 aatgatgggc gttaattaac tatttattaa ttagtttgta gatcaaggta ttgtcagtga
    16021 gacgaaaatc caggcttcgc tatttttggt gccgtcagct tagaggacag tcctcttagc
    16081 cccctccttt ccccgctcat tcattaaaca aatccatcgc cataaaatat ataaaaaagc
    16141 ccttgctttc taacgtgaaa gtggtttagg ttaaaagaca tcagttgaat aaacattcac
    16201 agagactttt atgacacgcg ttcaatttaa acaccaccat catcaccatc atcctgacta
    16261 gtctttcagg cgatgtgtgc tggaagacat tcagatcttc cagtggtgca tgaacgcatg
    16321 agaaagcccc cggaagatca ccttccgggg gcttttttat tgcgcggttg ataacggttc
    16381 agacaggttt aaagaggaat aagaaaatga cagacaacac tcgtttacgc atagctatgc
    16441 agaaatccgg ccgtttaagt gatgactcac gcgaattgct ggcgcgctgt ggcattaaaa
    16501 tcaatcttca cacccagcgc ctgatctcga tggcagaaaa catgccgatt gatattctgc
    16561 gcgtgcgtga cgacgacatt cccggtctgg taatggacgg cgtggttgac ctcgggatta
    16621 tcggcgaaaa cgtgctggaa gaagagctgc ttaaccgccg cgcccagggt gaagatccac
    16681 gctactttac cctgcgtcgt ctggatttcg gcggctgccg actttcactg gcaacgccgg
    16741 ttgatgaagc ctgggatggc ccgctctcct taaacggtaa acgtatcgcc acctcttatc
    16801 ctcacctgct caagcgttat ctcgaccaga aaggtatctc ttttaaatcc tgcttactga
    16861 acggttctgt tgaagtcgcc ccgcgtgccg gactggcgga tgcgatttgc gatctggttt
    16921 ccaccggtgc cacgctggaa gctaacggcc tgcgcgaagt cgaagttatc tatcgctcga
    16981 aagcctgcct gatacaacgc gatggcgaaa tggaagaatc caaacagcaa ctgatcgaca
    17041 aactgctgac ccgtattcag ggtgtgatcc aggcgcgcga atcaaaatac atcatgatgc
    17101 acgcaccgac cgaacgtctg gatgaagtca tcgccctgct gccaggtgcc gaacgcccaa
    17161 ctattctgcc gctggcgggt gaccaacaac gcgtagcgat gcacatggtc agcagcgaaa
    17221 ccctgttctg ggaaaccatg gaaaaactga aagcgctggg tgccagttca atcctggtcc
    17281 tgccgattga gaagatgatg gagtgatcgc catgagcttt aacacaatca ttgactggaa
    17341 tagctgtact gcggagcaac aacgccagct gttaatgcgc ccggcgattt ccgcctctga
    17401 aagcattacc cgcactgtta acgatattct cgataacgtg aaagcacgcg gcgatgaggc
    17461 cctgcgggaa tacagcgcga agtttgataa aaccacggtt accgcactga aggtgtctgc
    17521 tgaggaaatt gccgccgcca gcggacgcct gagcgacgag ctaaaacagg cgatggcggt
    17581 ggcagtaaag aatattgaaa ccttccacac tgcgcaaaaa ctgccgccgg tagatgttga
    17641 aactcagcca ggcgtacgtt gccagcaggt cacgcgccca gtcgcttcag ttgggttgta
    17701 tattcctggc ggctccgccc cgctcttctc aacggtatta atgctggcaa ctccggcgcg
    17761 tattgcaggt tgcaataaag tggtgctgtg ctcaccgccg ccgattgccg atgagatcct
    17821 ttatgcggcg cagctgtgcg gtgtacagga cgtgtttaac gtcggcggcg cacaggccat
    17881 tgccgcactg gcgtttggta ctgaatctgt gccgaaagtg gacaaaatct tcgggccggg
    17941 taacgccttt gtcaccgaag cgaaacgtca ggtgagccag cgtctggacg gtgcggcgat
    18001 cgatatgccc gcaggcccgt cggaagtgct ggtgattgct gacagcggcg ctacgccaga
    18061 tttcgtggct tctgatttgc tttctcaggc tgaacacggc ccggactcac aggtgatttt
    18121 actgacgccc gatactgata tggcgcatca agttgccgag gccgtcgaac gccaactggc
    18181 agaactgccg cgtgccgaaa ccgcccgcca ggcactgagc gccagccgcc tgatcgtgac
    18241 taaagattta gcgcagtgcg tagagatttc taatcagtac ggcccggagc acctgatcat
    18301 tcagacccgc aacgcccgcg agctggtcga tggcatcacc agcgccggtt cggtatttct
    18361 tggtgactgg tcaccggaat cggcaggtga ttacgcctcc ggcaccaacc acgttctacc
    18421 gacttacggc tacaccgcca cctgttccag cctcggcctg gctgatttcc agaagcgtat
    18481 gaccgtgcag gagctgtcga aagaaggttt ctccgcgctg gcttcgacca ttgaaacact
    18541 ggccgccgcc gagcgcctga ctgcccacaa aaatgccgtt actttgcgtg ttaacgccct
    18601 taaggagcaa gcatgaacac cgtgactatt accgatttag cgcgtgaaaa cgtccgcaac
    18661 ctgacgccgt atcagtcggc gcgtcgtctg ggcggtaacg gcgacgtctg gctgaacgcc
    18721 aacgaatacc ccacagccgt ggagtttcag ctcactcagc aaacgctcaa ccgctacccg
    18781 gaatgtcagc cgaaagcggt gattgaaaat tacgcgcaat atgcaggcgt aaaaccggaa
    18841 caggtgctgg tcagccgtgg cgcggacgaa ggtattgaac tactgattcg cgctttttgc
    18901 gaaccgggta aagacgccat cctctactgc ccgccaacgt acggcatgta cagcgtcagc
    18961 gctgaaacca ttggcgtcga gtgccgcact gtgccgacgc tggaaaactg gcaactggac
    19021 ttacagggca tttccgacaa gctggacggc gtaaaagtgg tctatgtttg cagccccaac
    19081 aacccgaccg ggcaactgat caacccgcag gattttcgca ctctgctgga gttaacgcgc
    19141 ggtaaggcga ttgtggttgc cgatgaagcc tatatcgagt tttgcccaca ggcatcgctg
    19201 gctggctggc tggcggaata tccgcacctg gctattttgc gcacactgtc gaaagctttt
    19261 gctctggcgg ggcttcgttg cggatttacg ctggcaaacg aagaagtcat caacctgctg
    19321 atgaaagtga tcgcccccta cccgctctcg acgccggttg ccgacattgc ggcccaggcg
    19381 ttaagcccgc aggggatcgt cgccatgcgc gaacgggtag cgcaaattat tgcagaacgc
    19441 gaatacctga ttgccgcact gaaagagatc cccatcgtgg agcaggtttt cgactccgaa
    19501 accaactaca ttctggcacg ctttaaagcc tccagcgcag tgtttaaatc tttgtgggat
    19561 cagggcatta tcttacgtga tcagaataaa caaccctctt taagcggctg cctgcgaatt
    19621 accgtcggaa cccgtgaaga aagccagcgc gtcattgacg ccttacgtgc ggagcaagtt
    19681 tgatgagtca gaagtatctt tttatcgatc gcgatggaac cctgattagc gaaccgccga
    19741 gtgattttca ggtggaccgt tttgacaaac tcgcctttga accgggcgtg atcccgcagc
    19801 tgctgaagct gcaaaaagcg ggctacaagc tggtgatgat caccaatcag gatggtctgg
    19861 gaacacaaag tttcccgcag gtggatttcg atggcccgca caacctgatg atgcagatct
    19921 tcacctcgca aggcgtgcag tttgatgaag tgctgatttg tccgcacctg cccaccgatg
    19981 agtgcgactg ccgtaagccg aaagtaaaac tggtggagcg ttatctggct gagcaagcga
    20041 tggatcgtgc caacagttat gtgattggcg atcgcgcgac cgatattcaa ctggcggaaa
    20101 acatgggtat taatggttta cgctacgacc gcgaaaccct gaactggccg atgattggcg
    20161 agcaactcac taaacgagac cgttacgccc atgtagtgcg caacaccaaa gagacgcaaa
    20221 ttgacgtcca ggtgtggctg gatcgcgaag gtggcagcaa gattaatacc ggcgttggct
    20281 tctttgatca catgctggat cagatcgcca cccacggcgg tttccgtatg gaaatcaacg
    20341 tcaaaggcga cctctatatc gacgatcacc acaccgtcga agataccggc ctggcgctgg
    20401 gtgaagcgtt aaaaattgcc cttggcgaca aacgcggtat ttgccgcttt ggttttgtac
    20461 tgccgatgga cgaatgcctt gcccgctgcg cgctggatat ctctggtcgc ccgcacctgg
    20521 aatataaagc tgaatttacc taccagcgtg tgggcgatct cagcaccgag atgatcgagc
    20581 acttcttccg ttcactttca tacaccatgg gcgtgacgct acacctgaaa accaaaggta
    20641 aaaacgatca ccaccgtgta gagagcctgt tcaaagcctt tggtcgcacc ctgcgccagg
    20701 ccatccgcgt ggaaggcgac accctgccct cgtcgaaagg agtgctgtaa tgaacgtggt
    20761 gatccttgat accggctgcg ccaatttgaa ctcggtgaag tctgccattg cgcgtcacgg
    20821 ttatgaaccc aaagtcagcc gtgacccgga cgtagttttg ctggccgata aactattttt
    20881 acccggcgta ggcaccgcac aagcagcgat ggatcaggtg cgtgagcgag aactgtttga
    20941 tctcatcaaa gcctgtaccc aaccggtgct gggcatctgc ttagggatgc aactgctggg
    21001 gcggcgcagc gaagagagca ccggcgtcga cttgctgggc atcatcgacg aagacgtgcc
    21061 gaaaatgacc gactttggtc tgccactgcc acatatgggc tggaaccgcg tttacccgca
    21121 ggcaggcaac cgcctgtttc aggggattga agacggcgcg tacttttact ttgttcacag
    21181 ttacgcaatg ccggtcaatc cgtggaccat cgcccagtgt aattacggcg aaccgttcac
    21241 cgcggcggta caaaaagata acttctacgg cgtgcagttt cacccggagc gttctggtgc
    21301 cgctggcgct aagttgctga aaaacttcct ggagatgtaa tgattattcc ggcattagat
    21361 ttaatcgacg gcactgtggt gcgtctccat cagggcgatt acggcaaaca gcgcgattac
    21421 ggtaacgacc cgctgccgcg cttacaggat tacgccgcac agggtgccga agtgttgcac
    21481 ctggtggatc tgaccggggc aaaagatccg gcaaaacgtc aaatcccgct gattaaaacc
    21541 ctggtggcgg gcgttaacgt ccctgtacag gttggcggcg gcgtgcgtag cgaagaagac
    21601 gtggcggcgt tactggaagc gggcgttgcg cgcgtggtgg tcggctccac cgcggtgaaa
    21661 tcaccagaaa tggtgaaagg ctggtttgaa cgtttcggtg ccgatgcctt agtgctggcg
    21721 ctggatgtcc gtattgacga gcaaggcaac aagcaggtgg cagtcagcgg ctggcaagag
    21781 aactcgggcg tttcactgga acaactggtg gaaacctatt tgcccgtcgg cctgaaacat
    21841 gtgctgtgta ccgatatctc gcgcgacggc acgctggcag gctctaacgt ctctttatat
    21901 gaagaagtgt gcgccagata cccgcaggtg gcatttcagt catccggcgg cattggcgac
    21961 attaacgatg ttgctgccct gcgtggtact ggcgttcgcg gcgtaatagt tggtcgtgca
    22021 ttactggaag gtaaattcac cgtgaaggag gccatcgcat gctggcaaaa cgcataatcc
    22081 catgtctcga cgttcgtgat ggtcaggtgg tgaaaggcgt acagtttcgc aaccatgaaa
    22141 tcattggcga tattgtaccg ctggcaaaac gctacgctga agaaggtgca gacgaactgg
    22201 tgttctacga tatcacagct tccagcgatg gccgtgtggt agataaaagc tgggtatctc
    22261 gcgtggcgga agtgatcgac attccgttct gcgtggcggg tgggattaag tctctggaag
    22321 atgccgcgaa aattctttcc tttggcgcgg ataaaatttc cataaactcg cctgcgctgg
    22381 cggatccgac gttaattact cgcctggcgg atcgttttgg cgtgcagtgt attgtggtgg
    22441 gtattgatac ctggtacgac gctgaaaccg gtaaatatca tgtgaatcaa tataccggcg
    22501 atgaaagccg tacccgcgtc actcagtggg aaacgctcga ctgggtacag gaagtgcaaa
    22561 aacgcggtgc cggagaaatc gtcctcaaca tgatgaatca ggacggcgtg cgtaacggtt
    22621 acgacctcga acagctgaaa aaagtgcgtg aagtttgcca cgtcccgctg attgcctccg
    22681 gtggcgcggg caccatggaa cacttcctcg aagccttccg cgatgccgac gttgacggcg
    22741 cgctggcagc ttccgtattc cacaaacaaa taatcaatat tggtgaatta aaagcgtacc
    22801 tggcaacaca gggcgtggag atcaggatat gttaacagaa caacaacgcc gcgaactgga
    22861 ctgggaaaaa accgacggac tgatgccggt gattgtgcaa catgcggtat ctggcgaagt
    22921 gctaatgctg ggctatatga acccggaagc cttagacaaa accatcgaaa gcggcaaagt
    22981 caccttcttc tcgcgcacta aacagcgact gtggaccaaa ggcgaaacat cgggcaattt
    23041 tctcaatgtg gtgagtattg ccccggactg cgacaacgac acgttactgg tgctggcgaa
    23101 tcccatcggg ccaacctgcc acaaaggcac cagcagctgc ttcggcgaca ccgctcacca
    23161 gtggctgttc ctgtatcaac tggaacaact gctcgccgaa cgcaaatctg ccgacccgga
    23221 aacctcctac accgccaaac tgtatgccag cggcaccaaa cgcattgcgc agaaagtggg
    23281 cgaagaaggc gtggaaaccg cactggccgc gactgtacat gaccgctttg agctgaccaa
    23341 cgaggcgtct gatttgatgt atcacctgct ggtgctgttg caagatcagg atctggattt
    23401 aactacggta attgagaacc tgcgtaaacg gcatcagtga gttgcggggt aagcggatgc
    23461 gatattgttg tcgcatccgg caaaaaaaac gggcaaggtg tcaccaccct gccctttttc
    23521 tttaaaaccg aaaagattac ttcgcgttgt aattacgcag agcgttacgc cccagcacaa
    23581 tccccgcgcc aaccatgccg cccagcagca ccgccagaat caaggtaatt gcctttttcg
    23641 ggctatcgcg acgaataggt aacgtcggtt tcattacata gcggtaagca tgaatatcaa
    23701 gtttatcaac gtcaagattg tcgatatcca gcaggttttg acgagtctga tagtagtttg
    23761 atgagaacac caacggacga gtcgcctcgt gcttaatcat cgactccagc gcttcgctcc
    23821 ccaaaaggaa catagtatct tgggttacgt cctgtgtctg ctgaatctgc ggctttgtca
    23881 cctgcgcctg attcgcatac tgcaacgctt cctgaatctg acggatacgc agatctttct
    23941 gctcctgcgc gactacttcc tgagttctta aagagtcctg caagtttttc cgtcccagag
    24001 caatgttgtc tttgagatcc ttttctagct cttgattcac tttttcatca acttgctgaa
    24061 tgtattgggc caacttcatt tgtgcgccct ctgctgtttg cccaacataa gaaacagtca
    24121 atggtaattg ctggttctta acagaaggtt cgatggtaag tttctctggc tcttcctgat
    24181 tatccagcgt ttctgctaat gcagagaagg cagaactgaa gcgaccaatt aacgtctcct
    24241 gcaaatccga tactttcggt gcagcctgac catatataac attcatggca ttgttatagc
    24301 cagcaatttg ccccacgtcg ggctgagtga taattgctgt tgacgtccat ttctccttcg
    24361 ctactgccaa ataaccaata gccagggcaa tagctacaat gacggaaatg atgattgtca
    24421 tcttgccacg ccacaactgc actagtaaat caatcaaatc aatctgttcc gggtcatggt
    24481 tttgcccaga aacattatta ttttctactc tcatcattac cctaactgac ggaaatttcc
    24541 aagtccgcat agtttagcga taatccccgt gaatgctata ggataagtga taaaaataat
    24601 gaattgttgc gtaagtaaca aaagacaatc agggcgtaaa tagccctgat aacaagatgt
    24661 tagtcgctgc caaagagatc gcgggtgtag accttatctg ccacatccct aagctcttct
    24721 gccatacggt tggaaataat gacgtcggct tgttgtttga aggtggcgag atcacgttcc
    24781 aagcgagagt tgaagaatga gtcttctttc atcactggct cgtagatgat cacttcaacg
    24841 cctttcgcct tgatacgctt cataatcccc tgaatagaag acgcacggaa gttatctgaa
    24901 ccgctcttca taatcagacg ataaataccc accacttgcg gtttgcgtga caaaatggca
    24961 tcggcaataa aatccttacg cgtgcggttt gcatcgacaa ttgccgagat caggttattc
    25021 ggcacagact gatagttcgc cagtaactgc ttggtatctt tcggcaagca ataaccacca
    25081 taaccaaacg acgggttgtt gtaatggttg ccaatacgcg gatcgagaca aacgccttcg
    25141 ataatctggc gagtattcag acctaaactt tctgcgtagc tatccagttc gttaaagtac
    25201 gccacgcgca tcgccaggta ggtgtttgca aaaagtttaa tcgcttctgc ttcagtggag
    25261 tcggtaaaca gggtcgggat attttgctta atcgcgcctt cctgtaacaa cgcagcaaaa
    25321 cgttctgcgc gttctgaacg ctcacctatg acaatacgtg aaggatggag attatcgtaa
    25381 agggctttac cttcacggag aaattccgga gagaaaataa tattttcagt acgatatttc
    25441 ttatgcatcg cttcggtaaa accaacggga accgttgatt tgatgaccat aaccgcataa
    25501 ggatttatct caactacgtc tttaagtact gattctacac tggatgtatt gaaataatta
    25561 gttttaggat catagtcggt tggagtggcg atgatgacat aatcagcatc ccggtaggct
    25621 tcatttttat ctaatgtggc attaaagtgt attttatctg attgcaaaaa ctgctgaatt
    25681 tccttatcaa caataggaga tatccgatca ttcagcatag caacgcgtga cggtaaaata
    25741 tctaatgcca caacctcatg attttgtgcg attagaagcc cgtttgacaa gcctacatag
    25801 ccagtaccgg aaatggtgat tttcatttca tgctctcaga attaacttaa ctgtgaatca
    25861 tgatgttttt agcatcctga taagagctaa aagttttaac gctacgttgt cagatggtca
    25921 acgcaacgaa aaacaattca gattttcttt ataagaatag ctgatattta ttaagttaat
    25981 attaagcagt gaaatttagt ctataagatt attggcaaaa aaagcccagc aatatatgcc
    26041 gggcctcaat ttattgttgt ttaagtcaga ttaatccagc cattcagtat ggaacacacc
    26101 ttctttatca atgcgcttat aagtatgtgc accgaaatag tcacgctgtg cctggatcag
    26161 gttcgcaggc agaacagcgg cacggtagct gtcataatag gcaaccgcag cggcgaaggt
    26221 gggaaccggg ataccgttct gtactgcata agcaacaaca tcacgcagcg cctgctgata
    26281 gtcatcggca atttgtttaa agtacggagc cagcagcagg ttagcgatct gcggattttc
    26341 ggcatatgca tcagtgattt tctgcaggaa ctgcgcacgg atgatgcagc cagcacggaa
    26401 aattttcgcg atttcgccgt agttcagatc ccagttgtac tcttcagacg ccgcacgcag
    26461 ctgagagaag ccctgagcgt aagaaacgat tttgcccaga tacagagcac gacgaacttt
    26521 ttcgatgaac tcacctttgt cgccagctgg ctgcgcttgc gggccagaga gaactttaga
    26581 tgcggcaaca cgctgctctt tcagagaaga gatataacgt gcaaacacag actcggtaat
    26641 cagcgacagc ggttcgccga gatccagcgc gctctggctg gtccatttac cggtaccttt
    26701 gttagccgct tcatccagga tcacatcaac caggtagtta ccgtcttcat cttttttagt
    26761 gaagatatct ttggtgatgt cgatcaggta gctgctcagt tcaccgttat tccactcggt
    26821 aaaggtctgc gccagttctt cgttagagag attcaggccg cctttcagga gagaataggc
    26881 ttcagcaatc agctgcatat caccgtattc aataccgttg tgaaccattt ttacatagtg
    26941 acccgcgcca tcggcaccaa tataggtaac gcacggttcg ccatcttcag caacggcggc
    27001 gattttggtc aggatcggag caaccagttc ataggcttct ttctggccac caggcataat
    27061 ggaaggacct ttcagcgcgc cctcttcacc gccggaaaca ccggtaccga tgaagttaaa
    27121 gccttcggca gaaagctcac ggttacggcg aatggtgtcc tggaagaagg tattaccacc
    27181 atcaatgatg atgtcacctt tatcgaggta tggcttgagg gaatcaatag cagcatccgt
    27241 gccagcacct gctttcacca ttaacaggat gcgacgaggc gtttccagag attcaacaaa
    27301 ctctttcacc gtatagtaag gaaccagttt cttgcctgga ttttcggcaa tcacttcttc
    27361 cgtcttctca cgggagcggt tgaaaacaga gacggtataa ccacggcttt cgatgttgag
    27421 cgctaggttg cgccccatca ctgccatacc gacgacgccg atctgttgct ttgacattgt
    27481 ttactcctgt cagggtgata ctgcgaaaca taactcaggt caatgtagat gctatattaa
    27541 ctcaggatac ataaaatcga taatttttac cgttgacaaa aataccatag agcattcatg
    27601 cttttatata accgttcgga ttcttttgtt gccaattcca ggcatctcga accatttctt
    27661 ccaacccacg cttcgctttc catccaagtt ccctataagc cttagaagga tcagaccaac
    27721 attcggcgat atctccaggg cgtctttcac tcaaaacgta aggtacggat cgaaaggtta
    27781 ctcgttcaaa agtggtcaaa agatccaaaa cagaataccc ctttcctgtt ccgaggttgt
    27841 aaactttatg attgggacct tcgtttctat gttccaacgc agcaacatga ccttcagcga
    27901 gatccattac atggatataa tcacggacac cagtgccatc ctttgttggg taatctcttc
    27961 catatactga aacttgttta tatttaccaa ttgccacctg acaaacatat ggcataagat
    28021 tatttggaat accatttgga tcctcaccaa tctctccaga gaaatgagca cccacagggt
    28081 taaaatacct caaaaccgtc actctaaagc tcgaatttgc ccttgtaaca tcatctaata
    28141 ttttttcaat cataagttta gaagtgccat aaggatttgt agtcatccca ataggtgcat
    28201 tttcagtaac tggcatggta ttcgagttac cgtatactgt tgcagatgag ctaaaaatta
    28261 aattataaat acctcttttc atcatttcat taataagaac aaggctagct acaacattgt
    28321 tattatagta atgaattggt tttgatatag actccccaac agatttagaa ccagcaaaat
    28381 gaataaccgt atgaatatca tgattagaaa aaata
//
LOCUS       DAZFFE010000057        28362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFFE010000057 DAZFFE010000000
VERSION     DAZFFE010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.057"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..204
                     /locus_tag="ACHJ69_002642"
     CDS             <1..204
                     /locus_tag="ACHJ69_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015597.1"
                     /translation="ADESMMQMKDVIWPAAEKEAYDKMKGMNATVVDVDKSAFKERVK
                     PLYDEFKAKDAQSAKNLELVESM"
     gene            246..728
                     /locus_tag="ACHJ69_002643"
     CDS             246..728
                     /locus_tag="ACHJ69_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO5015598.1"
                     /translation="MRTLTHILNKILAGCCCIILAIMVFCVTWQVIARFIFNAPSTVL
                     DEFTQILFMWMILLGGVYTAGLKKHLAIDLLAQKLPAASVLTLDSFIQIIITVFAVIF
                     MIYGGNIVVEKAQHVGQISPVLKWPMDKVYWVMPVSGLILVWYSVMNIIDNYRKRNSH
                     "
     gene            739..2043
                     /locus_tag="ACHJ69_002644"
     CDS             739..2043
                     /locus_tag="ACHJ69_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO5015599.1"
                     /translation="MEFEYIYPVLILFGSFAVMLAIGAPITFAIGLSSLLSIITALPP
                     DAAISVISQKMTVGLDGFTLLAIPFFVLAGNIMNTGGIARRLVNLAQALVGRLPGSLA
                     HCNILANTLFGAISGSAVASAAAVGGIMSPLQEKEGYDPAFSAAVNIASAPIGLMIPP
                     SNVLIVYSLASGGTSVAALFLAGYLPGILTAVALMFVAALYARRHHYPVAERINYRQF
                     LQVFRESIPSLMLIFIIIGGIIAGVFTPTEASAIAVIYSLALAMIYREITLKKLNDIL
                     LDSVVTSSIVLLLVGCSMGMSWAMTNADVPELINELITRVSDNKWVILFIINIILLIV
                     GTFMDITPAILIFTPIFLPIAQHLGIDPIHFGIIMVFNLTIGLCTPPVGTILFVGCSI
                     GKVSIDRAIKPLLPMFLALFVVMAIICYFPQLSLMLPGLFST"
     gene            complement(2114..3526)
                     /gene="ftsP"
                     /locus_tag="ACHJ69_002645"
     CDS             complement(2114..3526)
                     /gene="ftsP"
                     /locus_tag="ACHJ69_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO5015600.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(3601..4338)
                     /gene="plsC"
                     /locus_tag="ACHJ69_002646"
                     /gene_synonym="parF"
     CDS             complement(3601..4338)
                     /gene="plsC"
                     /locus_tag="ACHJ69_002646"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO5015601.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(4573..6831)
                     /gene="parC"
                     /locus_tag="ACHJ69_002647"
     CDS             complement(4573..6831)
                     /gene="parC"
                     /locus_tag="ACHJ69_002647"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO5015602.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDERRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLNIHVGKRKIKLRPEELQKVAGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(6969..8576)
                     /gene="ygiS"
                     /locus_tag="ACHJ69_002648"
     CDS             complement(6969..8576)
                     /gene="ygiS"
                     /locus_tag="ACHJ69_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5015603.1"
                     /translation="MYTQNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDKWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSITGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKVLLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(8685..9167)
                     /gene="ygiV"
                     /locus_tag="ACHJ69_002649"
     CDS             complement(8685..9167)
                     /gene="ygiV"
                     /locus_tag="ACHJ69_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO5015604.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTERRLETDIYVPLA
                     "
     gene            complement(9220..9612)
                     /gene="ygiW"
                     /locus_tag="ACHJ69_002650"
     CDS             complement(9220..9612)
                     /gene="ygiW"
                     /locus_tag="ACHJ69_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO5015605.1"
                     /translation="MKKFAAVIAVMALCSTPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            9764..10423
                     /gene="qseB"
                     /locus_tag="ACHJ69_002651"
     CDS             9764..10423
                     /gene="qseB"
                     /locus_tag="ACHJ69_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO5015606.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            10420..11769
                     /gene="qseC"
                     /locus_tag="ACHJ69_002652"
     CDS             10420..11769
                     /gene="qseC"
                     /locus_tag="ACHJ69_002652"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO5015607.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLKANGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            11879..12460
                     /gene="mdaB"
                     /locus_tag="ACHJ69_002653"
     CDS             11879..12460
                     /gene="mdaB"
                     /locus_tag="ACHJ69_002653"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO5015608.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIIRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            12491..12805
                     /gene="ygiN"
                     /locus_tag="ACHJ69_002654"
     CDS             12491..12805
                     /gene="ygiN"
                     /locus_tag="ACHJ69_002654"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO5015609.1"
                     /translation="MITVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GL"
     gene            complement(12850..13737)
                     /locus_tag="ACHJ69_002655"
     CDS             complement(12850..13737)
                     /locus_tag="ACHJ69_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO5015610.1"
                     /translation="MIQKDKVRVDIFGERFRTRASQLTPGLRVVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(13734..14681)
                     /locus_tag="ACHJ69_002656"
     CDS             complement(13734..14681)
                     /locus_tag="ACHJ69_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO5015611.1"
                     /translation="MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLAACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(14692..15729)
                     /locus_tag="ACHJ69_002657"
     CDS             complement(14692..15729)
                     /locus_tag="ACHJ69_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO5015612.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLLLLGGLFLLAVGILIFGLMQGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(15738..16721)
                     /locus_tag="ACHJ69_002658"
     CDS             complement(15738..16721)
                     /locus_tag="ACHJ69_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO5015613.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGVTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGFVLALYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(16718..17527)
                     /locus_tag="ACHJ69_002659"
     CDS             complement(16718..17527)
                     /locus_tag="ACHJ69_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5015614.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
     gene            17901..20042
                     /locus_tag="ACHJ69_002660"
     CDS             17901..20042
                     /locus_tag="ACHJ69_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFO5015615.1"
                     /translation="MAMFTPSFSGLKGRALFSLLFAAPMIHATDSVTTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHATRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDVLYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(20106..21998)
                     /gene="parE"
                     /locus_tag="ACHJ69_002661"
     CDS             complement(20106..21998)
                     /gene="parE"
                     /locus_tag="ACHJ69_002661"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO5015616.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(22027..22608)
                     /gene="yqiA"
                     /locus_tag="ACHJ69_002662"
     CDS             complement(22027..22608)
                     /gene="yqiA"
                     /locus_tag="ACHJ69_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO5015617.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(22608..23435)
                     /gene="cpdA"
                     /locus_tag="ACHJ69_002663"
     CDS             complement(22608..23435)
                     /gene="cpdA"
                     /locus_tag="ACHJ69_002663"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO5015618.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADARFQPDTASEGY"
     gene            complement(23460..23882)
                     /gene="yqiB"
                     /locus_tag="ACHJ69_002664"
     CDS             complement(23460..23882)
                     /gene="yqiB"
                     /locus_tag="ACHJ69_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO5015619.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(23883..24512)
                     /gene="nudF"
                     /locus_tag="ACHJ69_002665"
     CDS             complement(23883..24512)
                     /gene="nudF"
                     /locus_tag="ACHJ69_002665"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO5015620.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIIVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            24717..26198
                     /gene="tolC"
                     /locus_tag="ACHJ69_002666"
     CDS             24717..26198
                     /gene="tolC"
                     /locus_tag="ACHJ69_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO5015621.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            26346..27017
                     /gene="ygiB"
                     /locus_tag="ACHJ69_002667"
     CDS             26346..27017
                     /gene="ygiB"
                     /locus_tag="ACHJ69_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO5015622.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            27023..28183
                     /gene="ygiC"
                     /locus_tag="ACHJ69_002668"
     CDS             27023..28183
                     /gene="ygiC"
                     /locus_tag="ACHJ69_002668"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO5015623.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIAAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
BASE COUNT         7203 a         7486 c         6924 g         6749 t
ORIGIN      
        1 gccgatgagt caatgatgca gatgaaggac gtcatttggc ctgcggctga aaaagaagcc
       61 tacgacaaaa tgaaagggat gaacgcaaca gttgttgatg ttgataaatc cgctttcaaa
      121 gaacgcgtca aaccgttata cgatgaattc aaggcgaaag atgcacaatc agccaaaaac
      181 cttgagctag tagaaagtat gtaaccctaa tgggcaggta attcctgcct tttcatggaa
      241 gacttatgcg aacactcact catattctta ataagatcct tgccggatgt tgctgcatta
      301 ttctcgccat tatggtgttc tgcgtaacct ggcaagttat tgcacgtttt atttttaacg
      361 ctcccagcac tgtgcttgat gagttcaccc agattttatt tatgtggatg attttgcttg
      421 gcggtgtata tactgcgggt ttaaaaaaac atctggccat tgatttgttg gctcaaaaac
      481 ttccggctgc atctgttctg acgcttgaca gttttatcca aattattatt acggtctttg
      541 cggttatttt catgatctat ggcgggaata ttgttgttga aaaagcacaa catgttggac
      601 aaatctctcc agtattaaaa tggccaatgg ataaagtata ttgggttatg cctgtcagtg
      661 ggcttattct ggtctggtat tcagtaatga atattattga caactatcgt aaacggaatt
      721 ctcattaacg agggaaatat ggaatttgaa tatatctacc ccgtcttaat tttatttggc
      781 agttttgccg tcatgctggc aataggtgcg ccaattactt ttgcgattgg tctttcttcg
      841 ctgttatcta ttattactgc cttgccaccc gatgccgcca tttctgttat ttcacaaaaa
      901 atgactgtag ggctggatgg ctttacgcta ttagccattc ccttcttcgt gttagccgga
      961 aacattatga ataccggtgg tatagccaga cgactggtta acctggcgca agcattagtt
     1021 gggcgtcttc ctggctcact ggctcattgt aatatcctcg cgaatacact gtttggtgcg
     1081 atttcaggtt cagccgttgc gtcagccgca gcggtaggtg ggattatgtc accactgcaa
     1141 gaaaaagagg gatatgatcc ggcgttttca gcagcggtta atattgcctc tgcccccatt
     1201 ggcctgatga tcccaccgag caatgtgttg attgtttatt ccctcgccag tggcgggact
     1261 tctgtcgctg cgttgttctt agctggatat ctgccgggca tcctcaccgc tgttgcctta
     1321 atgtttgtag ctgcacttta tgcacgacgt caccattacc cagtggccga acgcatcaat
     1381 taccgtcagt ttttacaggt attccgcgaa tcaattccta gcctgatgct tatttttatc
     1441 attattggcg gtattatcgc aggggtattt acgcccacag aagcatcagc aatagcggta
     1501 atttatagtt tagccctggc gatgatttac cgggaaatca ctcttaagaa gctcaatgat
     1561 attctgttag attcggtagt aaccagttca attgttctgt tactggtagg ctgttcaatg
     1621 ggaatgtcat gggccatgac gaatgctgat gttcctgagt tgatcaacga actgattacc
     1681 cgcgtttcgg ataacaaatg ggtcattctg tttattatca atatcattct gttgatcgtc
     1741 ggtaccttta tggatatcac gccggcgatc ctgatattta cacctatttt cctgccgatc
     1801 gcccagcatc tggggattga tccgattcac ttcggcataa ttatggtttt caacttaacc
     1861 attggccttt gcacaccacc tgtcggcacc attctgtttg tggggtgcag cattggtaag
     1921 gtcagtatcg acagggcaat aaaaccatta ctgccgatgt ttctggcatt gtttgtagta
     1981 atggcaatta tttgttattt cccgcagctt agtctgatgc tgccaggatt attttcgacc
     2041 tgatacacaa tgaagactgc atactgagta attttcaatg ccccaccact ctgtggggca
     2101 ttgaattggt ccattacggt accggattga ccaacagttg cccaatcgag ccacggtccg
     2161 ccatttccag cgtctgactg ttgaagtaga acgggaagtg cgcccaggaa ggctgaccga
     2221 aataaacaag cagctccact tgtccgtcaa cccaaacggt atctttccag cctctgtctt
     2281 ccgggaacgg catcgcgcca ttcacgttac ggatctggaa catcacgcct tcaatatgga
     2341 acgcttgcgg ctcgtccgcg cgcaccgtcc agcgttccca cgttccttgc tgcgcagtaa
     2401 catcaatacg gttgacgtcc cacaactgcc cattaatccc cgggtcatca cccagactga
     2461 tatcacgact tcgaatcggc gaaccggcca tgatttcagt tggcagcaag cgcatcggaa
     2521 gactgtctgt gaccagcggc agaaggccgg ttgggcgcag cgttagcacc agcgtagaaa
     2581 ccagaatgct ggatggctca aagaagccac gaatacgatc aacaatgctc gccgcttcgc
     2641 cacaggtaat cgacacttca tcaccgttgc tcatatccac cagaatctca cgacgctcgc
     2701 ccggtgccag cgaaagttgc ttcaccgata caggagcggg caggaatccc tgatcgccag
     2761 aaatcacatg taacgggcga ccatcgctca tctgtagttg atagcgacga gagttcgacg
     2821 cgttcaacag acgcaagcgc acccagccac gcgagacttc aacgtacggg ctttgtacac
     2881 cgttaaccag cagcgtatca ccaacaaagc cgccgcttcc cggttcgtta tattctggcg
     2941 taccaaagtt atccagccgt ttatcctgaa taatgaccgg aaaatcatcc acaccataat
     3001 ggttggggat aggcagcgat ttgctgactt catcttccac cagccacatt ccggcaaggc
     3061 cgttatagac ctgctgagcc gtgcggttgg gagtattggc gtgataccac agagtagctg
     3121 cgttctggcg aatgggcagt acgggtgccc agtcagcgtt tggcgacatc atccgtgccg
     3181 gaccgcccat cagcgggcca ggtacctgta gcccggccac cgtcattgag acattttctg
     3241 tcaggcggtt gctgtaaata agcttaacat cgtcgccctt ccagacgcgg atagtcggcc
     3301 ccaggtaacg accattgatt ccccaaaccg acgcgcgtgt ccctggcgta aatgaccagt
     3361 gcgcacgttg tacagtcata aacagcggtt gcccacggcg agattcaagc agcggcggaa
     3421 cgggtagcgg ttgctgttgc ccggctgcgc tggccttcag gggaacagcg cctgcacaaa
     3481 gtgcaatccc cgatgcctga atgaactgac gccgactgag tgacatattt gctccatgta
     3541 aaactggcta aagtaatacc gtgcaggaaa gtgttccccg caaaaataca gattgccgac
     3601 ttaaactttt ccggcggctt cgcgttctgc gacttcttta tcgagctcgg cgattttttg
     3661 ttccattatc gaacgacaat gggcagccaa ctcgcgaacc tgatctttgc catactgact
     3721 gacgtcaatt ggcggcagca tttcgacaat caccagaccg ttgtgtagcc gattaagatt
     3781 aatcttattc gaagttgtag agacgcacac gggtataatc gggacgcccg ccgcaattgc
     3841 cgcgtgaaat gccccggtct tgaacggcag caggccgcga ccacggctgc gggttccttc
     3901 cgggaacatc cagatggaaa tacggcgttt tttgaagtga ttcactactt ccgcaatagt
     3961 gccgtgagct ttagtgcgat tgtttctgtc gatcaataag ttgccggtta accagtacaa
     4021 ctgcccgaag aaggggatcc acagcaagct ttttttacct accgtcactg tcggcggctg
     4081 cacaatgttc gatgccgtca ccatgtcata attgttctgg tggttagcaa tatagatagc
     4141 attgccgtag ctttcagcgt ctgcaggttt gcggcactcc actttcaggc caaaaagcgg
     4201 cgcaagacgg ccaaacatat gcccaaaggt ggccacatgt ttcgggttac gcgggctgaa
     4261 aaggcagtaa atggagccga atacacagac taagatgctg taaatcacgg taataataag
     4321 acgaaagata tatagcataa caccctctga aggtctgaga gactggcatt gtacgtcacc
     4381 tgaaaatcag gttagggcat tgttaaaagc cgctctctgg aaaagcgtat tgttaatcgc
     4441 aacgcatgaa taaacaacgg tttcaaggga atttttatcg tttccggggg ttgaatacgc
     4501 ggccggatga cgacttaacg tttcatccgg cgttccttgc aagcgggagg aaacagcgcc
     4561 ctccccggca tattactctt cgctatcacc gctgctggca cggcgaggag agtcgatctc
     4621 aacacgatcg atacgctgca aaccgcgcat caacgtaccg cggcgtccac gttcgccagc
     4681 gactttctgt agctcttccg gacgcagttt aattttgcgt ttcccaacat gaatgttcag
     4741 cgtgctttgc ggcggcagaa cgtacagttg cgcaagacca tcctcgccac gcgcggcttc
     4801 tgccgatgga atgttgataa tcttgttgcc tttgcccttc gacagctgcg gcagatcgct
     4861 taccgggaac atcaacatac ggcctgcctg agtgattgcc agcagcatat cggaagcatc
     4921 ttcaatcacc accggcggca taacatgggc attttccggt aaggtgatca aagctttacc
     4981 tgcacggtta cgcgccacca gatcgttaaa ggtgcagacg aaaccgtaac ccgcatcgga
     5041 agccatcagc agtttctgat cgtcgctttc catcagcata tggtcaacgg tcgctccagg
     5101 cggcaacgtt aatttgccgg tgagcggctc gccctgacca cgcgccgacg gcagcgtaat
     5161 cgggtcgatg gcatagctac gaccggtgga atcaacaaac actaccggtt ggttgctctt
     5221 acctttcacc gccgctttga agctatcgcc cgctttataa ttcaggcccg gcgcgtcgat
     5281 atcatggcct ttagcgctgc gtacccagcc catctgcgac agcacaatgg tgacaggttc
     5341 agacggcagc atgtcgtgct cgctcatcgc tttcgcttct tcgcgttcct gcaacggcga
     5401 acgacgttcg tcaccgtagg cttgcgcgtc tgcctgcagt tctttcttca gcaggttatt
     5461 cattttacgc tcggaagcca aaatgccctg caactggtcg cgctcttttt ccagctcact
     5521 ctgctcaccg cgaatcttca tctcttccag tttggcaaga tgacgcagtt tcagttcgag
     5581 gatcgcttcc gcctgggttt ccgtaaggcc aaaccgcgac atcagcgccg gtttcggttc
     5641 atcttcatta cgaatgatct caatcacttc gtcgatattg agaaacgcca ccagcaaacc
     5701 ttcgaggata tgcaggcgct tgaggacttt ctccagacga tagttcagtc ggcggcgcac
     5761 ggtatcgcgg cggaacacca gccattcgga gaggatttcc agcaggtttt tcaccgccgg
     5821 acgaccatcc agaccgatca tattgaggtt aatgcgatag ctcttttcca gatcggtggt
     5881 agcaaagagg tggttcatca cctgatccat atccacgcgg ttggaacgcg gcacaatcac
     5941 cagacgggtc gggttctcgt ggtcagattc atcgcgcaga tcgtcaacca tcggcagctt
     6001 tttgttgcgc atttgcgcag caatttgctc cagtacgcgc gcacctgaaa cctgatgcgg
     6061 caatgcgctg ataaccaccg cgccatcttc tttcttccac accgcgcgca tacgcactga
     6121 accacgtccg ttctcgtaga ttttacgaat ctcggcgcgc gaagtgataa tttccgcttc
     6181 agtcggataa tccggcccct gcacgatatc cagcagctga tcgagcgtgg ttttcggctg
     6241 gtcgattaat gcgattgccg cctgagccac ttcacgcagg ttatgcggtg gaatatcggt
     6301 cgccatgccg acggcaatac cggtggtgcc gttaagcaaa atgtttggca gacgggcagg
     6361 cagcattttc ggctcctgca aagtgccgtc gaagtttggc acccagtcag ccgtcccctg
     6421 ccccagctcg ctcaatagca gctcggaata tttcgacaac cgggattcgg tgtaacgcat
     6481 tgccgcgaac gatttcggat cgtccggcgc gccccagttc ccctgaccat caaccagcgg
     6541 ataacggtaa gagaacggct gcgccatcag gaccatcgct tcataacagg cactatcgcc
     6601 gtgcggatgg tatttaccca gtacgtcacc gacggtacgg gccgattttt taaatttggc
     6661 gctggcattc aggcccagct cagacatcgc atacacaatg cggcgctgaa caggtttcag
     6721 accatcacca ataaacggca acgcacggtc catgatcacg tacatggagt agtttaagta
     6781 ggcgttttcc gtaaattcat gtagcgcaag gcgctctgcc atatcgctca ttaattctga
     6841 ttcctcaact tattcgccag ccttcaaagg ggcaatattg ccggagatac tacattatct
     6901 gccgcgtcga gtcacaggag atcagcgtga atagcgccgc cggacgtgct ccgacggcga
     6961 taaaggcatc aatgtgcctt gatatacaac tctttgctgt agacataatc ctggggatta
     7021 tgcagaggaa aaccgccaac gtagggttta agcagtttga ttaacggctg atagtagatg
     7081 ggaatcagcg gtgcctgctg gttgatgatc acttctgcct gctgatacaa cgcattacgc
     7141 tttgtcgcat cagtgatctg cgtggcctgg tttagtaagg cgtcatactg cgcatttttc
     7201 cagtgaccga cgttttcttc actatcgctt ttgagcgtgt tcaggaagct ggaggcatca
     7261 ttgtacgtcg catcccacga ctgccgagac agcataaaat caccggctcg tcgggcatca
     7321 agataggttt tccactccat tgtgcgcagc gtcacctgtg cacctagcca ttttttccat
     7381 tcggaagaca acgctatcgc ggtcttttca tgcagatcgt acttgttgta gaacagctca
     7441 aagcgtagcg gatgagaggc gtcgtatccc gcctgtttca gcaagacttt tgccatcgcg
     7501 acacgctcac tcattggctt ttgcagttca tcgaacgtcg tcgcgctaaa gccttttacc
     7561 tctggcggcg tcagcgtggt tgcgggcgtt ctcaacccca gtaccttttg cgcaataagc
     7621 tgtcgatcaa ccgtaagata tagcgcccga cgcacccgca catcgttaaa tggcggtttc
     7681 tcaaggttaa agttgtaata ttcgctgttc agacgcggga taattcgtag ctcgccaggc
     7741 agtgattttt caatggcggg aatttgctgc gccggaaccc aggtgagatc gacctctccc
     7801 gcgcgatagc ggttatagcc ggtgatcgaa ttatccagcg ccagatactc aacctgttgc
     7861 aatactgtat gttgcgcatc gcggtacttt ggatttttgc gtgcagtaat cttttcgtta
     7921 actacccatt tatcaagcac aaaggcaccg ttgtaaacca tgttctctgg cttactccag
     7981 ctatcgccat gtttagcgat gacatgatga ggaaccggga acagcgtcgg ccaggcgagc
     8041 atcgtcgtga accaaggaac tggctgctca agcgtaactt caagagtacg atcatccgtc
     8101 gctttgacac ccagcgatgt aacatctgct ttacccgcaa caatagccgc ggcattgtta
     8161 atgtgcgcct gtgccagata gccagcaaaa gggcttgccg ttttcgggtc aaccgcacgt
     8221 tgccagccga ggacaaaatc ctctgccgtc agaggctgac cgtctgacca ctgcaaaccg
     8281 ctacgtagat ggaaaatata gcgcttgccg ccgtcaagta tctcccagcg ttcagcctga
     8341 gcgggctgca cctggccttc accgtccatc cataccagac cttcaaacag atccagcaca
     8401 atctgcgcgg cagtattctc ctctaccttt tgcgggtcga gcgtgcctgg gtcgctatga
     8461 ttgttgtaac gaaagacttg ttgcggggcg agcggtgtgt tggcgggaac gtcagcagcg
     8521 tagagaggag ccgcacttac caggctgacc agccataata aattttgcgt atacatggta
     8581 ttccttaccg gagaaattat aagcccgaca acaatacgcc tgtggcattg tttgctcaaa
     8641 cttatcgcga gtgatttggc tcacactccg gtaaagattc cccatcacgc taacggtaca
     8701 taaatatccg tttccagtcg ccgctctgtc atcccttcgg caagattggt gtagtgaaac
     8761 aggatcgggg ctttgcgcat tttttcgccg cttgcaggta gccagtggcg aatgatgccc
     8821 catatcgtgt gtgaaatatc gtccagctcg ccaacatggc gggcaacagc ataacgccca
     8881 ccggtaagtt cgccattact cacgccataa cgattatcgg ggatcggttc actaacgctg
     8941 ccgcagatat caaagcgaaa cgcttccggt gcggtggtgg ctgggtcgtc ccaggcgacg
     9001 ccaaaagtct ggctttggtt aacaggagaa agccccgtct ctttacgcca catgataaat
     9061 ttcgccacgc tgtagttgag caactcaggg ctacagcggt gcggcaacat cgccacaggg
     9121 attgatggga aatcgataat atttacatcc agtgtcaggt ttgtcatttt gcctccctgc
     9181 gaaattattc ccgggaacgc gtcccgggag cggtaacaat tacggattta ctttgcggat
     9241 ctgtttgacg tcaatttcaa cagagttcca gtctttatcg acttccccct gaatctcaac
     9301 cgtatctttc ggcgtcaccg tcacgccgtt ccagcgtttg tgatcgatat ccacattgat
     9361 agtaccgctg gcatctttga acacgtagag atcgtcagag atgcgttcaa cgatattgcc
     9421 gcgcagggtt acccaggtgt cgtcacgcag ggattttgcg ctttctacgg tcgttacgct
     9481 gccgttcggc ccctggaatc ctccggcctg actttgcgtt gccgatgggc cagaaaaacc
     9541 tccctgctct gctgccataa ccggtgtact gcacagggcc attactgcga ttactgctgc
     9601 gaattttttc atgtttatta ctccctttaa tgtctgtttc cgagcattta acaagatagt
     9661 ccttaacaac ttcttaaggg aaaaaaataa aatttaatgc tgtacagagc gcgttacaac
     9721 acggtttact ggcagcaaat acggttatcg cagggatgaa aaaatgcgaa ttttactgat
     9781 agaagatgac atgctgattg gcgacggcat caaaacgggc ctcagtaaaa tgggttttag
     9841 cgtcgactgg tttacacaag gtcgtcaggg aaaagaggcg ctatatagcg caccttatga
     9901 tgcggtgatc ctggatttaa ccttaccggg catggatggt cgcgatattt tgcgcgaatg
     9961 gcgagaaaaa ggtcagcgtg agccggtatt gatcctgact gcgcgcgatg cgctggcgga
    10021 acgtgtagaa ggactgcgtc tgggagctga cgattatctg tgtaaacctt ttgcgttgat
    10081 agaagtcgcc gccaggctgg aagctctgat gcgccgaacc aacggccagg ccagcaacga
    10141 gctgcgccac ggcaacgtca tgctcgaccc cggcaaacgt atcgccacgc tggctggcga
    10201 acccttaacg ctgaaaccaa aagaatttgc cctgctggaa ttactgatgc gtaacgctgg
    10261 tcgggtactg ccgcgcaaac tgattgaaga gaaactgtat acctgggacg aagaggtcac
    10321 cagtaatgcc gttgaagtgc atgtgcatca tctgcgacgc aaactcggca gtgattttat
    10381 tcgtaccgtt catggtattg gctacacatt aggtgagaaa tgaaatttac ccaacgtctt
    10441 agtctgcgag tcaggctgac gctaatcttt ttaattctgg cctcggtgac ctggctgctt
    10501 tccagctttg tcgcctggaa acaaacaacg gataacgtcg atgagttgtt cgacacccaa
    10561 ctgatgctgt ttgccaaacg gttaagtacg ctcgatctca acgaaatcaa cgcggcggat
    10621 cgcatggcac agacgccaaa taaattaaaa cacggtcatg ttgatgacga tgctctgacc
    10681 tttgccatct ttacccacga cggcagaatg gtccttaacg atggcgataa cggagaagat
    10741 attccttata gctatcagcg ggaaggtttt gctgacgggc aactggtcgg tgaagacgat
    10801 ccctggcgtt ttgtctggat gacctcacct gatggcaaat atcgcatcgt tgtgggtcag
    10861 gaatgggaat accgtgaaga catggcgctg gcgattgttg ccggacaatt gatcccgtgg
    10921 ctggtcgcac tgccgattat gttaatcatc atgatggtac tactgggtcg tgaactcgcg
    10981 ccgctgaaca aactggcgct ggcactacgt atgcgtgacc cagactcgga aaaaccacta
    11041 aacgcgacgg gcgtacccag cgaagtgcgc ccactggttg agtcgctaaa tcaactgttc
    11101 gcccgcacac atgcgatgat ggttcgtgaa cgacgcttta cctccgacgc agctcacgaa
    11161 cttcgtagcc cgttaacggc gctgaaagtg caaaccgaag ttgctcagct ctctgacgat
    11221 gatccgcagg cgcggaaaaa agcgctgctc caattacatt ccgggatcga tcgcgctact
    11281 cgtctggttg atcaactgct cacgctatcg cggctggact cactggataa ccttcaggac
    11341 gtcgcggaga tcccgcttga agatctcctg caatcgtcgg tgatggatat ttaccacacg
    11401 gcgcagcagg cgaaaattga cgtgcgactg acactcaaag ccaacggcat caaacgcacc
    11461 ggacaaccgc tattgctaag tttgttggtg cgaaatttgc tggataacgc cgtgcgttac
    11521 agtccacaag gtagcgtggt agacgtcacg ctgaatgccg ataatttcat cgtgagggat
    11581 aacggccctg gtgtgacacc agaggcactg gcgcgaattg gcgaacgctt ctatcgccca
    11641 cccggacaaa ccgctaccgg cagtggacta gggctatcga ttgtccagcg aatcgccaaa
    11701 ttgcatgaca tgaatgttga atttgggaat gcggaacaag gtggatttga ggcgaaggta
    11761 agctggtaag ggtaagactt ttgctaaatt cgcaaaagac tttgcacatt ttgctaattt
    11821 caccgtaccg ctctgtgacg tactatagtc ggcaaacgtc tcaccttgag gtaaaaaaat
    11881 gagcaacatc ctgattatca acggcgcgaa aaaattcgcc cactccaatg gtcaactgaa
    11941 cgacaccctg accgaagtcg cggatggcac actgcgcgac ctcgggcatg atgtccgcat
    12001 tattcgcgcc gacagcgact acgatgtcaa agcggaagta caaaactttc tctgggctga
    12061 tgtggtgatc tggcagatgc ctggctggtg gatgggcgcg ccgtggacag tgaaaaaata
    12121 tattgatgat gtattcaccg aaggtcacgg gactctgtat gccagcgatg gtcgtacccg
    12181 caaagatcca tcaaaaaaat acggttccgg cggcctggta cagggcaaaa aatatatgct
    12241 ttctctgacc tggaacgcac caatggaagc cttcaccgaa aaagatcagt tcttccacgg
    12301 cgttggtgtt gacggtgtgt acctgccgtt ccataaagca aaccaattcc ttggcatgga
    12361 accgctgccg acatttatcg ctaatgacgt gataaaaatg cctgatgttc cccgctatac
    12421 tgaagaatat cgcaagcatc ttgtggaaat ttttggttaa ctagagctct ggctttagaa
    12481 ggagttaacc atgattacag taatcgcaga aatccgtact cgtcctggtc aacatcaccg
    12541 tcaggcagtg ttggatcagt ttgctaaaat cgttccaacc gtactgaaag aagaaggttg
    12601 ccacggctat gcgccaatgg tggattgcgc tgctggcgtg agtttccagt ctatggcacc
    12661 ggattctatc gtgatgattg agcagtggga aagcatcgcg catcttgaag cgcatctgca
    12721 aaccccgcac atgaaagcat atagcgaagc cgtaaaaggt gacgtgctgg agatgaatat
    12781 tcgtatcctg cagccggggc tttgacattt ctaaaaagag gccggtgctg ctaccggtct
    12841 cagcccagat tagccattct ttcttcccgt tttcagtcct cgatggaaat cgttaattcg
    12901 cttaatggtt ttaaccgcac gctgcggcac tgcggaagct accgaccaga caatcatttc
    12961 cagacacaac aataccgtac catgtagcgg cgttttccct ttttcatccc cgcgcggaac
    13021 atgaatcacg atactggcgt ctttgctaaa acgtgaatcc agcgcgtttg tcagcaaaat
    13081 aacagggatc cccagccgcc tcgcctcacg aagcgttgtc agcccttccc ggtgcgctga
    13141 tttttgcgcc atcatgatca gaacatcgcc acgttgaagc gcgatcagtt gctcggcaag
    13201 gccaataccc gtacggttta gtgccgtggc aggcaaacct atgcggttga atagtcgtgc
    13261 ggtatagtcc gccagaatac ccgaagcgcc aatgccaaaa attgcgactt gccgtgcctg
    13321 gaccaacaac gctacagctt gcgcaagcgc gtggcggttt tcaggctctg acaggacatt
    13381 acaggtatac aaatgccctt caagaacaaa gtcgattgcg gtgttaacat cgctcgtcaa
    13441 gttactcacc gtcgtagaca tcttttcgct ggaactaagg gctggaccaa gccactgttc
    13501 cagagtgcgt ttcagatcac gcagcccggc aaaccccagg gcctgtatag ccctgataac
    13561 tgtggcatcg gaagtgttca acgtggccgc aatttccata gccgtttgct cgagtaccac
    13621 ttcacgatgc tcattaatgt agctggcgac aactcttaac ccaggcgtta actgactcgc
    13681 gcgagtgcga aaacgctcac caaaaatatc aacccgcact ttatctttct ggatcattta
    13741 gcctccggta gaggtcgccc tgcaatttgt gattgtatct gcgcagccat cagccccaga
    13801 gatgcaaagg agttagaaat tgcctcttca cgagaaatca gcacataacg cccggaacga
    13861 caggcagcca gaaactgaca ccatcccggc atgacttttt ccattgctgc cagctcatcc
    13921 tgaggtttac caccggtgtc accgcgccag gttgcaaaaa caaaatcggc atccagttca
    13981 ggtaaacgct ccgcgctcac atccattcgc ccaccttcag gaatgctctc aatcagcggc
    14041 ggaaaacgaa aacccgcatc gcgtaatacc cgccccagcg aatgatagct atgcatcaca
    14101 ttaatttttc cctggtttgc ctgaatcacc gataccgtta ttttttggct gtccagcgtc
    14161 gccttcagcg cattgatttg tgcctgatag cggcgttcaa gtatcgccag ctgcgactgc
    14221 gttcctgtaa gttctgccag cttgcgatag atttctggcg cacccccttt gagatgatca
    14281 atgctcaccg tgggcgcaat tttttcaagt tgttcaatgg gcgtattacg ggtcggttct
    14341 gtgatgatca ggtcaggctt tgccgccaca atcgcttcaa tatcaatatc agcggtacca
    14401 ataaaggcga tcgatgaatt atcaaagtcg acacctgtca ataacgcgcc agatcgaatg
    14461 aaatggctac cgtcaggccg agttcgcccg tggctggcga caggaggtac accgagctca
    14521 atcagaggaa tagtgatatc aagatcgtgt aaagagacaa ttctctttgg atgagcagga
    14581 acagtgactt tgcgccctaa atcatcagta aatacctgaa caggttctgt tgcacgggaa
    14641 aaaaatgaca acagtatcag cagagaaaaa aataaacgca tataactccc gttaaaaact
    14701 gttcctgcgc tgccagagca gtagcaaaaa aaatggtcca ccaatcagtg agataatgat
    14761 tccggcaggc agttgcaaag gcaaaaaagc caaacgacca atgctatccg ccagaataac
    14821 taacaacgcc cccgataccg cgctccccag cagtaatgtc gtctgcccgc cacgtagaat
    14881 aaaacgtgat atatggggtg ctatcaatcc gacaaaaccg atgttaccaa cacacgaaac
    14941 gcagacggct gtcaggatta tgggtgcggc gacacgtaac aacgccagac gagaactatt
    15001 cacccctaac ccggttgcaa cctgatgacc aagcagagcg atatccgcag tcctggcggt
    15061 gaacagcaat aaaagagcgg ctggcaacat ccaacaggct gaaataccga ctaacatcca
    15121 gttagctgca tgcaggcttc ccgataacca catcaatgca gtttgtacat ctcgaacatc
    15181 tgcagtggtc ataaatacac cgataccggc ggcgaaaaac caggaaacac caataccaat
    15241 cagaacaaat cgcgggcggg aaatatcgcg cgcacaaaaa atcacgatta aagccaccaa
    15301 cagaccacca gcaagaccag ccactggacg ccagaacatt cccagcattg gaaactggaa
    15361 aatcagccac agcaccgcca cgctacatcc ttctttgacg ccgattaaac caggatcggc
    15421 aaggccgtta cgcgcaatgg attgcatcgc ggcccccgcc atacctaaca tggccccgca
    15481 tagcaatgcc atgatgactc tcggcaagcg aatatcctgc acaatataac gtgcgtctgt
    15541 gcttacgttt tctggagcaa acaacgctcg tcctacttcc gatgccggaa cagagaaaga
    15601 accctgcatc agaccgaata tcagtattcc aaccgcaagc aggaacagcc caccaagcaa
    15661 cagcagattt ttagggcgca ccagtgtcga aaacttacca acacgtagtg ggcgtagccc
    15721 gacgttcatt atccgactca tttgaacatc ctcgtcgcca taatgacaaa aacaggcgca
    15781 ccaactaacg ccgtcatgac accggtcgct aactcatggg gcgtgaaaag cgtccgggca
    15841 ataatatctg ccagtaataa caaaagcgcg ccgacacaag ctgaaagagg gagcagaacg
    15901 cgcagatccg ctgataccag acgccggaca atctgcggta ccaacaggcc gacaaaacca
    15961 atggggccag caatcgatac ggctgcgcca cagagcaacg cgatcgcaag cagggtaaat
    16021 gttcgcgtgc ggagtacaga gacgcccagc ccctgcgcca tgcgatcgcc cagtgccaac
    16081 atgttaaggg agggtgcaag gtaaagggct aacacaaatc cccccagaga aaaccaggcg
    16141 gacgtaccca gcgtcgccca atcctgcccc gctaaatcac ctgctagcca tgtccgcatc
    16201 gccagcagtg tttgttcgtc aagaatcagg attgcagccg ttattgagga cacgaacgcc
    16261 gatagcgcca cgccgcataa agtaactttc attggcgtta cccctgaacg ccctgcggag
    16321 gaaagcagca atatcaacag gaacaataac gcgccccccg tagatgccag cagtgggcgt
    16381 ccaaccggaa aggccagtcc aagcgcactg gcggcaacga cagcaagagc ggctccggca
    16441 tttaagccta aaatatgtgg ctcccccaat gggttacgaa tgacagcctg caacaaagca
    16501 ccagcaacac caagagaagc accggtaagc aatgctgccg ctaaacgcgg caaacggagt
    16561 cttacaatta cgttgtgatc gaagttttgc ggatcaaaat gaagaaatgc ctgcaacacc
    16621 gtttgtggtg caataaatcg ggcaccaatc cctggatata ccacgcatcc caccagcagt
    16681 aaggcgagga aaattacagt aaccagccct gtgcgcatca tactttttct acaccacgaa
    16741 acggcatgaa gaacggcttt cccgtcagag gatttattga tgcgtgaaca tcaacgtcaa
    16801 agacagcttt taccagttca ggcgtgcaat gctccccctc attcaacaca cgcaccactt
    16861 tcccctggcg taaaaatatc aacgtatcgc cataattgac ggcaaaattc agatcatgca
    16921 acaccacgac gactgtacgc ccgtgatgac gcgttaaatc atgtaataac tcaagaattt
    16981 caacctgata acgtaaatca agccaggttg tgggttcatc cagcaggata taaggcgtct
    17041 tttgcgccaa caccatcgca atccagcaac gctggcgctg cccaccagag agtttttcaa
    17101 caggtaaatg tgcaaactcc tgcgtaccgg ttaatttcag ggcctcttcc accgcctgct
    17161 catcggcgtc gctccattga cgtatgaaat tttgccaggg aaaacgccca cgcgacacca
    17221 gttcataaac ggttaagccc tccggcaata atggcgactg cggcaagatc ccaagtctac
    17281 gggataacgc tttcgttggt tgctcgtgaa tcgctttgcc atcaagcaat atcgaaccgc
    17341 ccatgggttg caagatacgt gcaatggtgc tgagcaacgt cgactttcca cagccgtttg
    17401 ccccaaccag aacggtcatt ttttctgtgg gaacggagaa agaaacacca tcaacaatga
    17461 tcttcttgtg atacccggca gaaacattgt cgaggatcag cccctgccct tttgcgttat
    17521 gtgccacgtg tacgccaact taatcaaaga caaacaaaaa taaaactcat tctcctttga
    17581 gagtttgacg ccacaaaatg tagtagtcaa gcagttaaaa gccacacaaa aacaaggtaa
    17641 ataacaggta aactattttt atacataaaa ataaaaacat taaatatcaa taaattaaaa
    17701 gcaatcaagt tgtataaact tttcataaaa acacatgtag caattttttg ttatttctca
    17761 tttactacta cattactgcg tgattgaatg attctcaatt acatatccga tggcgaaata
    17821 acaaacggat agtgcttagt gagcaataag aaacaatttt cactcgcgac aatccgcgag
    17881 tttttgggat aacagactaa atggctatgt tcacaccttc attctcagga ctcaaaggtc
    17941 gggcgctctt ttcactgctt tttgcggcac cgatgattca tgcaacagac tctgtaacga
    18001 ccaaagatgg cgaaacaatc actgttacag cagatgcaaa taccgcaact gaggcaaccg
    18061 atggttatca acctctgagc acctccacgg cgacattaac cgatatgccg atgctggata
    18121 tcccgcaggt ggtcaatacg gttagcgatc aggttctgga aaaccagaat gcgacaacgc
    18181 tggatgaggc gctttataac gtcagtaacg tggtacagac caatacatta ggcgggactc
    18241 aggatgcttt tgtacgccgt gggtttggcg caaaccggga tggctccatc atgaccaacg
    18301 gtctgcgaac cgtacttcct cgtagtttca acgccgcaac agagcgtgtg gaagtgctaa
    18361 aaggcccggc ctccacgctg tatggcattc tcgatcctgg cggactgatt aacgtcgtga
    18421 ccaagcgccc ggaaaaaaca ttccatggtt cggtttcagc cacctcctcc agttttggtg
    18481 gcggcactgg gcaacttgat atcacaggtc ccattgaagg cactcagctg gcgtatcgcc
    18541 ttaccgggga agtgcaggat gaagattact ggcgaaactt cggtaaagag cgcagtacat
    18601 ttattgcccc gtcactcacc tggtttggtg ataatgcaac agtaaccatg ctctattccc
    18661 atcgggacta taaaactccg ttcgatcgtg gaacgatttt cgaccttacg acgaaacagc
    18721 ccgtaaacgt tgatcgaaaa atacgttttg acgaaccgtt taatattaca gatggtcagt
    18781 ccgatctggc gcaactcaac gcagaatatc atctcaatag ccagtggaca gcgcgctttg
    18841 attacagcta cagccaggat aaatacagcg ataatcaggc gcgtgttacc gcgtatgatg
    18901 caacgacagg aacactgaca cggcgtgttg atgcaactca gggatctacc cagcgtatgc
    18961 atgctactcg tgcggatctg caagggaatg ttgatattgc cggattctat aatgagattc
    19021 tgggtggggt gtcatatgaa tattatgatc ttctgcgtac agatatgatt cgctgtaaaa
    19081 aagctaaaga tttcaatata tacaaccctg tttatggtaa taccagcaaa tgtacaacgg
    19141 tttcggcgtc ggacagtgat cagacgatca aacaggagag ctactcagcg tatgcacagg
    19201 acgtactcta tctgaccgat aactggattg ccgtcgccgg gatccgatat cagtattaca
    19261 cgcagtatgc gggtaaaggc cgtcctttta atgtcaatac tgacagccgc gatgaacaat
    19321 ggactcccaa actggggttg gtctacaaac tgacgccatc ggtatcctta tttgccaatt
    19381 attcgcaaac atttatgccg cagtcgtcaa ttgccagcta cattggcgat cttccaccag
    19441 aatcatctaa tgcttacgaa gtcggggcaa aattcgagct attcgatggt atcaccgcag
    19501 atattgcgct gtttgatatc cataaacgta atgtgttgta taccgaaagt attggtgatg
    19561 aaaccatcgc caaaacggca ggccgtgttc gttcaagagg ggtagaagtc gaccttgcgg
    19621 gagcattaac tgaaaacatt aatatcattg ccagctacgg ctataccgat gctaaggttc
    19681 tggaggatcc tgattatgca gggaaaccat tgccgaatgt tcctcgtcat accggttcgc
    19741 tattcctgac ctatgatatt cataacatgc caggcaataa tacgctgaca tttggcggtg
    19801 gtggacatgg tgtaagccgt cgttcggcaa ccaatggggc tgactattat ctgccaggct
    19861 attttgttgc cgatgccttc gccgcataca aaatgaaatt gcagtatccg gtcacactgc
    19921 aattaaacgt caaaaacctg tttgataaaa cgtattacac ctcttccatc gccacaaata
    19981 atctgggcaa ccagattggc gatccgcgtg aagtgcaatt cacggtgaaa atggaatttt
    20041 gatcaaaaaa gcccggtata cacttgtata ccgggctatg ttttattctt ctggcgggag
    20101 gattatcaga cctcaatctc tgccatgtcg cctttctctt gcaaccagtt gcggcgatct
    20161 tccgagcgtt tcttcgccag cagcatatcc atcatcgcgt cagtacgctg atcgtcttca
    20221 tcatcgatag tcaactgcac cagacggcga gtgttcggat caagcgtggt ttcgcgcaat
    20281 tgcatcgggt tcatttcccc gagaccttta aaacgctgga cgtttggctt gcctttcttg
    20341 cgttttaatt gctcaagtac gccctctttc tcttcttccg tcagcgcgta ataaacctct
    20401 ttcccgaggt caatacggta gagcggtggc agtgcaacgt aaacgtgacc gtgtttcacc
    20461 aatgcgcgga agtgttttac aaacaaagcg cagagcagcg tggcaatgtg cagaccatcg
    20521 gagtcagcat ccgccaggat acagatcttg ccgtaacgaa gctggctcaa atcgtcgctg
    20581 tcaggatcga taccgatagc taccgaaata tcgtgcactt cctgcgaagc cagcacttcg
    20641 tcggaagaga cttcccaggt gttaaggatc ttacctttca gtggcatgat cgcctgatat
    20701 tcgcgatcgc gcgcctgctt ggcagatccg cctgcggagt caccttccac aaggaatagt
    20761 tcggtacggt taagatcctg cgcggtacag tcagccaatt taccaggcag cgccgggccg
    20821 ctggttagct ttttacgcac cactttttta gccgcacgca tacgacgctg ggcgctggaa
    20881 atcgccatct ctgccaacag ctccgccgcc tgaacgttct ggttcagcca caggatgaag
    20941 gcatctttca ccacgcccga aacgaatgcc gcacactgac gcgaagagag acgttctttg
    21001 gtctgcccgg caaactgcgg atcctgcatt tttactgaca gcacataggc gcagcgatcc
    21061 cagatatctt ccgccgacag ctttacacca cgcggcagaa tgttgcggta ttcacagaac
    21121 tcacgcatcg cgtccagcag gccctgacgc aggccattaa catgggtacc gccctgcatc
    21181 gttgggatca ggttgacgta gctttcggtc agcagttcac cgccttccgg cagccacagt
    21241 agcgcccagt ccaccgcttc agtatcgcca gcgaaattac cgataaacgg tttttctggc
    21301 agcgtcggta aaccgtttac cgcttccgcc aggtaatcat tcagaccgtc ctgatagcac
    21361 cagcgctgtt cggtgttgtt gatctcatct ttaaaagtga tctcaacgcc cgggcacagt
    21421 accgctttgg ctttcagcac atgcgtcagg cgtgaaacag aaaaacgcgg gctgtcaaag
    21481 aaggtttcat ccggccagaa gtgcacgctg gtaccggtat tgcgtttacc gcaagtgccg
    21541 acaacctgta aatcttgcac cttttcgcca ttttcaaagg cgatgttata gatctgacca
    21601 tcgcggcgta cgttaacttc tacgcgcttc gacagggcgt taactaccga aatccccacg
    21661 ccatgcaggc cgccagagaa ctggtaattt ttgttagaga atttaccgcc cgcgtgcaga
    21721 cggcaaagaa tcagttcaac cgccggtacc ccctcttccg ggtgaatatc taccggcatc
    21781 ccgcgcccat cgtcaatcac ttctaacgac tggtcggcat gtaagattac atccacgcgt
    21841 tttgcgtggc ccgccagtgc ttcatcgaca ctgttatcaa taacttcttg ccccaaatgg
    21901 ttagggcgag tggtatcggt atacatcccc ggacggcggc gaaccggctc aagcccggtg
    21961 agtacctcaa tggcatcagc gttataagtt tgcgtcatgg tttaagttag caattcgagt
    22021 tgatcgtcag agatggtgca gaccaagaaa atcgacgatc gggttgaaat aatcttcgaa
    22081 gcccgtgaat gcgtggttgc cgccttctat gacagtctgg cggcaggaag cgtagtacgc
    22141 caccgcctgg cggtaatcca gcacttcatc tcccgtctgt tgcagcagcc agatcaaatc
    22201 cggcgcttcc agcgggtcaa tctgcatgac tttgagatcg taaatatggc gtgactctag
    22261 cacatattgc tgcccggtgt aggggttctc gttctgaccg agatagtccg tcagcagttc
    22321 aaacggacgc accgccgggt tgaccaccac tgcgggcagc ataaaacatt gcgacaacca
    22381 ggtggcgtaa tatcccccca gtgacgaacc gacaataccc agcgaatcgc cgccatgttc
    22441 caggacaata gattccagca gttctgccgc gtcagaagga tacgggggca actgcggaat
    22501 gatcatctca acgtcagggt gatgttccgc cagccagttt tttaacaagc tcgcttttgc
    22561 agagcgcggc gagctgttga aaccgtgtaa ataaagaagc gtagacatca gtagccttct
    22621 gaagcggtat caggttggaa gcgtgcgtcc gccaggcgat gcacctcggt ggtcagcgtg
    22681 ccatcagcat gtaactcgag agtacgccag ccgggcgcga tggtgtccag cgtaaagtta
    22741 gaacagtgcg gcttaaactg cacacaggtc gacggcgttg ccagcaagcg gcgaccgttc
    22801 cagtcgagat ccagctcctg atgaatatga ccgcacagca agtatttgac gtgcggaaac
    22861 ttcgccagca cggtatccag ttcgcccgcg ttacgcaggc tgtgttgatc aagccaacta
    22921 caacccgcag gtagcggatg atgatgcagc agcagcaacg tatggcgttc tggcgcatcg
    22981 gccagtttac gttccagcca ctcaagctga aactcgctca gctcaccgtg cggcacgcca
    23041 aacacctggc tatccagcaa caggatttgc cattgctcac caataaacac gcgcttcgcc
    23101 ggggagatac ccgcatcctg taacgcgctg tacatcgcgg gctggaaatc gtggttgccc
    23161 ggcagccaga cgcagggcgc acgaaaactt gcgatgcctt cagcgaaatg ctgataggcc
    23221 gcagaggatt gatcctgcgc taaatcacct gtcgcgacaa tcaggtcgaa ttcgtgctgg
    23281 tgtggccgaa tcgcctccag caccgcctgg tagctttccc aggtgtttac ccctaacagg
    23341 gcttcgtgct tttgtgcaaa caggtgagtg tcggtaattt gtaaaatcct gactctggcc
    23401 tcaccagcca gaggaagggt taacaggctt tccaaatggt gtccttaggt ttcacgacgc
    23461 taataaaccg gaatcgccat cgctccatgt gctaaacagt atcgcaacca gtccgctaaa
    23521 aactgattaa tttgatgctt ttcgtcgcgt tgatgcaact ttttattagg ataatcatac
    23581 cgcgctttga agcgaaaaat ctgctggctt gaacacactt cagccaccat cgcgtcatga
    23641 tacagacgca ccgtcattga cggaaggctc cagtaactga tcgcgggcgc agtctgttct
    23701 attgtcacca gggtagtgta tcgggtcgat tccacaatcg tcagccgata ttgtgcgttt
    23761 gccacctgat agcttacagt ttcgccgggt gcgtcattgc gcggtaacaa acggcgcaat
    23821 tgtgaaaagt tcatctcgca caggcgcatc atttcaggaa agtcaggtgt gtaacgcttc
    23881 atttatgccc actcattttt taacgcttga tgatgcagct gcagccattg caaagcgatg
    23941 accgacgctg cgttgtcgat tttcccctct tctacccact ggtatgcctg ttcccggctt
    24001 accacatgaa cgcgaatatc ttcgttttca tcagccagac cgtgaatacc gcttgcggtc
    24061 gtggcgtcca cttcgcccac aataattgac gaacgctcac tggtgccccc cgggcttgcc
    24121 aggaaactta acaccggttt ggtccgtttg actatcagtc ccgcctcttc aatcgcttcg
    24181 cgacgggcaa catcttccac actttcaccc tcttcaatca tcccggcaac catctccagt
    24241 agccaggggg tttcgctggt gtcgtacgcg gcaatccgaa tctgctcaat cagcacaact
    24301 tcatcacgca ctgggtcaaa gggtagcaag actgcggcgt gaccgcgctc aaaaatttcc
    24361 cgccgtacct catgactcat ttgcccgttg aatagacgat gacgaaatct ataaagatct
    24421 aatgaaaaaa agccgcgata aagtgtttct cgtgcaataa tttctacatc gtttttgcca
    24481 aatgtaacgg gcaggttgtc tggcttaagc attgttaatg tcctggcact aatagtgaat
    24541 taaatgtgaa tttcagcgac gtttgactgc cgtttgagca gtcatgtgtt aaattgaggc
    24601 acattaacgc cctatggcac gtaacgccaa ccttttgcgg tagcggcttc tgctagaatc
    24661 cgcaataatt ttacagtttg atcgcgctaa atactgcttc accacaagga atgcaaatga
    24721 agaaattgct ccccattctt atcggcctga gcctctctgg gttcagttcg ttgagccagg
    24781 ccgagaacct gatgcaagtt tatcagcaag cacgccttag taacccggaa ttgcgtaagt
    24841 ctgccgccga tcgtgatgct gcctttgaaa aaattaatga agcgcgcagt ccattactgc
    24901 cacagctagg tttaggtgca gattacacct atagcaacgg ctaccgcgac gcgaacggca
    24961 tcaactctaa cgcgaccagt gcgtccctgc agttaactca atccattttt gatatgtcga
    25021 aatggcgtgc gttaacgctg caggaaaaag cagctgggat tcaggacgtc acgtatcaga
    25081 ccgatcaaca aaccttgatc ctcaacaccg cgaccgctta tttcaacgtg ttgaatgcta
    25141 ttgacgttct ttcctataca caggcgcaaa aagaagcgat ctaccgtcaa ttagatcaaa
    25201 ccacccaacg ttttaacgtg ggcctggtag cgatcaccga cgtgcagaac gcccgcgcac
    25261 agtacgatac cgtgctggcg aacgaagtga ccgcacgtaa taaccttgat aacgcggtag
    25321 agcagctgcg ccagatcacc ggtaactact atccggaact ggcggcgctg aatgtcgaaa
    25381 actttaaaac cgacaaacca cagccggtta acacgctgct gaaagaagcc gaaaaacgta
    25441 acctgtcgtt gttacaggcg cgcttgagcc aggacctggc gcgcgagcaa attcgccagg
    25501 cgcaggacgg tcacttaccg actctggatt tgacggcctc taccggaatt tctgacacct
    25561 cttacagcgg ttcaaaaacc cgtggtgccg ctggtaccca gtatgacgat agcaatatgg
    25621 gccagaacaa agttggcctg agcttctcgc tgccgattta tcagggcgga atggttaact
    25681 cgcaggtgaa acaggcccag tacaactttg ttggtgccag cgagcaactg gaaagcgcgc
    25741 atcgtagcgt cgtgcaaacc gtacgttcct ccttcaacaa cattaatgct tctatcagta
    25801 gcattaacgc ctacaaacaa gccgtagttt ccgctcaaag ctcattagac gcgatggaag
    25861 cgggttactc ggttggtacg cgtaccattg ttgatgtgtt ggatgcgacc accacgctgt
    25921 acaacgctaa gcaagagctg gcaaatgcgc gttataacta cctgattaac cagttgaata
    25981 ttaagtcagc tctgggtacg ttgaacgagc aggatctgct ggcactgaac aatgcgctga
    26041 gcaaaccggt ttccactaat ccggaaaacg ttgccccgca aacgccggaa cagaatgcta
    26101 ttgctgatgg ttatgcgcct gatagcccgg caccagtcgt tcagcaaaca tccgcacgca
    26161 ctaccaccag taacggtcat aaccctttcc gtaactgata acgacaacgg ggcttcggcc
    26221 ccgtctgaac gtaaggcaac gtaaagatac gggttatctg ccgcattctt cccccttctc
    26281 gcttcaattt cgaccagcca tcctctattc tgatgggtat ttaccactgg tcccggaaga
    26341 caaaaatgaa acggacaaaa tccatacgcc acgcatcgtt ccgcaaaaac tggagcgcac
    26401 gccatctgac accagtcgct ctcgcggttg ccactgtttt tatgctggct ggctgtgaaa
    26461 agagtgatga aacagtgtct ctctatcaaa atgctgacga ctgttcagct gcaaacccag
    26521 gcaaaagcgc cgaatgtacc accgcgtaca acaatgcgct gaaagaagcc gaacgtactg
    26581 cgccgaaata cgccacccgt gaagactgtg ttgctgaatt tggtgaaggt cagtgccagc
    26641 aggcaccagc ccaggctggc atggcaccag aaaaccaggc gcaggcccag caatccagcg
    26701 ggagtttctg gatgccgctg atggccggtt acatgatggg gcgtctgatg ggcggcggcg
    26761 cgggatttgc acagcagccg ctgttctcct cgaaaaaccc ggccagcccg gcttacggta
    26821 aatataccga cgcgaccggt aaaaactatg gcgcagccca gccaggccgc accatgaccg
    26881 taccgaagac ggcaatggca ccaaaaccgg cgaccactac tactgttacc cgtggcggtt
    26941 ttggtgaatc tgttgccaaa caaagcacta tgcagcgtag cgcaaccggc acctcttctc
    27001 gttcaatggg tggctgatac cgatggaaag agtcagtatt accgagcgcc cggactggcg
    27061 cgaaaaagcc cacgaatacg gtttcaattt tcacaccatg tacggcgagc cgtactggtg
    27121 tgaagatgct tactacaagt tgaccctcgc tcaggttgaa aagctggaag aagtcaccgc
    27181 cgaactgcac cagatgtgcc tgaaagtggt ggaaaaagtg atcgccagcg atgagctgat
    27241 gaccaaattc cgcataccaa aacacacctg gagttttgtg cgccagtcat ggctgacgca
    27301 ccagccatcg ctttattcgc gtcttgatct ggcatgggat ggtactggcg aacctaaact
    27361 tctggaaaat aacgccgata cgccaacgtc actatacgag gcggcgttct ttcagtggat
    27421 ctggctggaa gatcagctta acgccggtaa cttgccggag ggcagcgacc agtttaacag
    27481 tttgcaagaa aagctgatcg atcgcttcgt tgagctgcgt gaacagtatg gcttccagtt
    27541 gctgcatctc acctgctgtc gcgacacggt ggaagatcgc ggaaccattc agtatttgca
    27601 ggactgcgcg acggaagctg aaatcgctac cgagttcctc tacatcgacg atatcgggtt
    27661 aggtgaaaaa ggtcagttca cggatttaca ggatcaggtg atttccaacc tgttcaagct
    27721 gtatccgtgg gaatttatgt tgcgtgagat gttctccacc aagctggagg atgcaggcgt
    27781 acgctggctg gaaccggcgt ggaagagcat tatctccaac aaagcgcttc taccgctact
    27841 gtgggagatg ttcccgaatc acccgaacct gctgcccgct tattttgcgg aagatgatca
    27901 tccgcaaatg gaaaaatatg tggttaaacc gatcttctcc cgtgaaggcg caaacgtgtc
    27961 gatcattgag aacggcaaaa ccattgcagc agcggaaggt ccgtatggcg aagaagggat
    28021 gattgttcag caattccacc cgttaccgaa attcggcgac agctatatgc tgattggtag
    28081 ctggctggtg aacgatcaac ctgccggaat tggcattcgt gaagaccgtg cattgatcac
    28141 ccaggatatg tctcggtttt atccgcatat ttttgttgag taatacagct taatattgcc
    28201 ccgataagcg gggcaatctc ttataatcca ccattcataa ctcccatcaa tatataaaca
    28261 catacatatc caacctttat ttaacattaa tactcaattt aatgttaaat cgtcatatgc
    28321 aagcgatatt gtaattaaag taagtgaata catcacaaca ta
//
LOCUS       DAZFFE010000058        28287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFFE010000058 DAZFFE010000000
VERSION     DAZFFE010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.058"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(133..1038)
                     /locus_tag="ACHJ69_002669"
     CDS             complement(133..1038)
                     /locus_tag="ACHJ69_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5015624.1"
                     /translation="MFISDETLRVIHLVARHQSITTAAEQLNKVPSAISYTIKKAEES
                     LGVELFIRKGRYIELSPAGSYFIEHSKTILGDLEALKRNTVLIHDGIERELTIAVNNI
                     IPGDLLVAFIRDFERQFTSTTLTVDLEVYNGCWDALYSKRADLVYGAPHAVPSSEGII
                     SEPVGQMEWDFVVSPLHPLAAKRHPLENSELRHYPAVCIRDTSVNFPPMQAWLLEGQK
                     PIFVPDFATAIALIEQNVGIGYIPHHLALPLLNSGKLLKKPMREHKHATKLFLAARSD
                     GMGKACQWCIEYLRNPQLMTRFVFN"
     gene            complement(1116..2339)
                     /locus_tag="ACHJ69_002670"
     CDS             complement(1116..2339)
                     /locus_tag="ACHJ69_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO5015625.1"
                     /translation="MRKKEENLNTASGLRIAMILLGIAVTPVLLSSSSLGNQLSSSSL
                     ISVVLLGGVILTLLSAITISVGEKARLPTYGIVKYSFGEKGAIAINILMAISLFGWIA
                     VTANMFGHSVHDLLAQHGLEVPLALLVAAGCVIFVASTAFGFAVLGKIAQVAVPVIAL
                     VLCYILYVATHTEVAVPAAIVEMNTGVAVSTVVGTIIVLVATLPDFGSFVHNRKHALI
                     AAGVTFLVAYPLLYWAGATPSAISGQGSLLGAMAVFGAVLPAALLLIFACVTGNAGNM
                     FQGTLVVSTLLTRFPKWQITVALGILSAIVGSMDIMAWFIPFLLFLGIATPPVAGIYI
                     ADFFLYRRNGYQESVLAQESQIKVLTFAAWIIGAAVGFMTVKGLFTLTTIPSVDSILV
                     ACIAYAILSRASQHR"
     gene            complement(2365..2754)
                     /locus_tag="ACHJ69_002671"
     CDS             complement(2365..2754)
                     /locus_tag="ACHJ69_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HFO5015626.1"
                     /translation="MTTTLLAVNGTLMRGLELNPNMQKAGGIFVREDRTDAHYRLWSI
                     NDRHPGMIRVNEGGTHVDVEIWQLPLASFAALLMSEPAGLAIGKIKLADGSEVLGVLA
                     ENWLTEGQREITELGSWRKYTGHFHTV"
     gene            complement(2771..3727)
                     /gene="arcC"
                     /locus_tag="ACHJ69_002672"
                     /pseudo
     CDS             complement(2771..3727)
                     /gene="arcC"
                     /locus_tag="ACHJ69_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606533.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
     gene            complement(3720..5144)
                     /locus_tag="ACHJ69_002673"
     CDS             complement(3720..5144)
                     /locus_tag="ACHJ69_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO5015627.1"
                     /translation="MNTLFNQPLKVVNAGLHSFADNIQHAGGSAIALNWQPPAQGDID
                     AGLDLASLLRHPLVENANQIAMTRYLEAQPVLVDVMLAKEAIPAMAEQKRILHSGPPI
                     AWEEMCGPVKGAIIGAMLYEGWATSQQDAENQINAGEIDLAPCHHYHAVGPMAGIISP
                     SMPLWVVENKTNGHRTFSNFNEGLGKVLRFGANNDEVLNRLAWMRDELAPAMKAAIAQ
                     HGELELKPLMAQALHMGDEVHNRNAAATGLLIKRLLPALLACSLPQEHIQRVVAFITG
                     NDHFFLNLSMAACKAMMDAAANVPFSSMVTVMARNGVNFGIRLSSTGDRWFQAPANAV
                     EGLFFPGFGVDDAAADLGDSAITETAGVGGFAMASSPAIVKFVGGTPADATNNSRRMQ
                     AITLGGNPAFTLPALNFAPTAAGIDARKVTDRGILPVINTGIAHKQAGVGQIGAGITT
                     APMACFVAAVRALAEIVVKENHHG"
     gene            complement(5141..6700)
                     /gene="fdrA"
                     /locus_tag="ACHJ69_002674"
     CDS             complement(5141..6700)
                     /gene="fdrA"
                     /locus_tag="ACHJ69_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO5015628.1"
                     /translation="MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNL
                     EQLRDAGLGNEINASPNDLVIAVMGEEDICNEALALAQQRLTSKPDDETDSGIKTPEK
                     VSLEMALEAEPEANLALISVPGDYAAAEAIKALNLGMNVMMFSDNVSIGQEKSIKTLA
                     RERQRIVMGPDCGTAIVNGIPLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGAGIS
                     QALGTGGHDLSEEIGGISMLFALDALAQDDETRVIVLISKPPSPIVAQTILERAEACG
                     KPVVVNFLGANPHDLARPNITAATTLASAADIAVALLNVQPLPAVETDVPCDDLTMLQ
                     NACQRLPAHRQAIRGVFAGGTFCYEAQLICQQKGFSAASNTPVAGNRALANIWQSEDH
                     TLIDMGDDDFTRGKPHPMIDPTLRNQRLLNELNDSHTAVVLFDLVLGYGASTTPASEL
                     LDQLSHIDMNNVPLLIAHVCGTEADPQIRSQQISALQNAGVIIASSNAQAALWASTVA
                     QTQLQKKGLNA"
     gene            complement(6795..7655)
                     /locus_tag="ACHJ69_002675"
     CDS             complement(6795..7655)
                     /locus_tag="ACHJ69_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO5015629.1"
                     /translation="MVQVTLCASSLGYLFPQNETQDLRLHSAFKHAVSLYSDAGTFIT
                     LLCAQTYLNLPDAARVWLPECWDWRREIAHSDPIQLTPGLLRTPRFCVALDNATLWQS
                     PFVGRMLTFEAFPLVFQHYPTMASQRLLFCLEHNVQSTLHLPDSLTHQGLAIMEHPDA
                     LERQVPQLIGFGKGLTPDGDDYLLGYLAALWLWQLPAPLADHQYRLQQAIDQHAHNTT
                     DISRHYLERALQGYFSEPICQLLAQLVGSASAMTIASCAEQVMQFGATSGVDCLAGML
                     HGFRTLNTMN"
     gene            complement(7661..8329)
                     /locus_tag="ACHJ69_002676"
     CDS             complement(7661..8329)
                     /locus_tag="ACHJ69_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFO5015630.1"
                     /translation="MTQSIFQAQPFELPFDPRTTALVMIDMQRDFVEAGGFGEALGND
                     VSLVRTAIAPCTEVLAAARQKGIMVIHTREGHRADLSDCPPAKLTRGGKTFIGEPGPM
                     GRILVRGEAGHDIIPELYPVAGEPVIDKPGKGAFYQTDLHLILQNHGIKTLIVCGVTT
                     EVCVTTTVREANDRGYECIIPEDCVGSYFPEFQKYALEMIKAQGAIFGWVTDSKAIIA
                     GLEG"
     gene            complement(8586..9395)
                     /locus_tag="ACHJ69_002677"
     CDS             complement(8586..9395)
                     /locus_tag="ACHJ69_002677"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO5015631.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGLLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRRA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFTQYGVKK"
     gene            9657..10418
                     /gene="udp"
                     /locus_tag="ACHJ69_002678"
     CDS             9657..10418
                     /gene="udp"
                     /locus_tag="ACHJ69_002678"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO5015632.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            10559..11986
                     /gene="rmuC"
                     /locus_tag="ACHJ69_002679"
     CDS             10559..11986
                     /gene="rmuC"
                     /locus_tag="ACHJ69_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO5015633.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFVDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            12081..12836
                     /gene="ubiE"
                     /locus_tag="ACHJ69_002680"
     CDS             12081..12836
                     /gene="ubiE"
                     /locus_tag="ACHJ69_002680"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO5015634.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKTMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            12850..13455
                     /gene="ubiJ"
                     /locus_tag="ACHJ69_002681"
     CDS             12850..13455
                     /gene="ubiJ"
                     /locus_tag="ACHJ69_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO5015635.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            13452..15092
                     /gene="ubiB"
                     /locus_tag="ACHJ69_002682"
     CDS             13452..15092
                     /gene="ubiB"
                     /locus_tag="ACHJ69_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO5015636.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            15171..15440
                     /gene="tatA"
                     /locus_tag="ACHJ69_002683"
     CDS             15171..15440
                     /gene="tatA"
                     /locus_tag="ACHJ69_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO5015637.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            15444..15959
                     /gene="tatB"
                     /locus_tag="ACHJ69_002684"
     CDS             15444..15959
                     /gene="tatB"
                     /locus_tag="ACHJ69_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO5015638.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNETAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            15962..16738
                     /gene="tatC"
                     /locus_tag="ACHJ69_002685"
     CDS             15962..16738
                     /gene="tatC"
                     /locus_tag="ACHJ69_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO5015639.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIISVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            16780..17562
                     /gene="tatD"
                     /locus_tag="ACHJ69_002686"
     CDS             16780..17562
                     /gene="tatD"
                     /locus_tag="ACHJ69_002686"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO5015640.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAVTEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQELAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(17559..18047)
                     /gene="rfaH"
                     /locus_tag="ACHJ69_002687"
     CDS             complement(17559..18047)
                     /gene="rfaH"
                     /locus_tag="ACHJ69_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO5015641.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPSTPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            18214..19707
                     /gene="ubiD"
                     /locus_tag="ACHJ69_002688"
     CDS             18214..19707
                     /gene="ubiD"
                     /locus_tag="ACHJ69_002688"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO5015642.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            19753..20454
                     /gene="fre"
                     /locus_tag="ACHJ69_002689"
     CDS             19753..20454
                     /gene="fre"
                     /locus_tag="ACHJ69_002689"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO5015643.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(20727..21890)
                     /gene="fadA"
                     /locus_tag="ACHJ69_002690"
     CDS             complement(20727..21890)
                     /gene="fadA"
                     /locus_tag="ACHJ69_002690"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO5015644.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRHVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(21900..24089)
                     /gene="fadB"
                     /locus_tag="ACHJ69_002691"
     CDS             complement(21900..24089)
                     /gene="fadB"
                     /locus_tag="ACHJ69_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO5015645.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDTPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVIMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAVVDDLLAEVSHAKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            24279..25610
                     /gene="pepQ"
                     /locus_tag="ACHJ69_002692"
     CDS             24279..25610
                     /gene="pepQ"
                     /locus_tag="ACHJ69_002692"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO5015646.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPNSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            25610..26224
                     /locus_tag="ACHJ69_002693"
     CDS             25610..26224
                     /locus_tag="ACHJ69_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO5015647.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            26263..27714
                     /gene="trkH"
                     /locus_tag="ACHJ69_002694"
     CDS             26263..27714
                     /gene="trkH"
                     /locus_tag="ACHJ69_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO5015648.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFSLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            27726..28271
                     /gene="hemG"
                     /locus_tag="ACHJ69_002695"
     CDS             27726..28271
                     /gene="hemG"
                     /locus_tag="ACHJ69_002695"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO5015649.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         6639 a         7201 c         7582 g         6865 t
ORIGIN      
        1 aaaaaatgaa ggtgtgcagt gaacgtaatg attaacagac cggttaattt tcatgaatta
       61 cgtcaactgc acaaaaataa tttaaacggg tgctctaatt aaagcaagac ttatattttt
      121 atcattgtat tgtcaattaa atacaaatcg tgtcattaat tgtgggtttc ttaaatattc
      181 tatgcaccac tgacaggctt tgcccatacc atcggaacga gcggcaagaa ataacttggt
      241 ggcgtgttta tgttcgcgca ttggcttttt aagcaattta ccgctgttca ataacggcaa
      301 tgccagatga tgtggaatat aaccaatacc gacgttttgt tctattaagg cgattgccgt
      361 agcgaagtca ggaacaaaaa tgggtttttg tccttccaac aaccaggctt gcatcggggg
      421 gaagttaacg gaggtatcgc ggatacaaac cgctggataa tgacgcaatt cactgttttc
      481 taatggatgc cgcttcgctg ccagtgggtg gagtgggctg acgacaaaat cccattccat
      541 ttgtccgacc ggttcgctaa tgatgccttc actgcttggt actgcatgag gagcaccgta
      601 gaccagatca gcacgcttac tgtacagcgc atcccagcag ccgttgtata cctcaagatc
      661 taccgtcagt gttgttgatg tgaattgccg ttcaaaatct cgaataaatg ccaccagcaa
      721 atcaccagga ataatattgt ttaccgcaat tgtcagttca cgctcaatac catcgtgaat
      781 cagcactgtg ttgcgcttta atgcttccag gtcaccaaga atagttttgc tatgttcgat
      841 gaagtaactt cccgctggcg ataattcaat atagcgtccc ttacgaatga acagttcaac
      901 gcccaggctc tcttccgctt tcttgatagt gtaactgatg gcggaaggga ctttatttaa
      961 ctgctcggct gcggtggtaa tgctctggtg tctggcgacc agatgaataa cacgaagcgt
     1021 ttcgtctgag atgaacataa ggctccctgg tcagtggtaa agtggctgaa tctctccaga
     1081 ttcagccgta atcattgcct gagtataccc aggaattagc ggtgttgact tgcccgactg
     1141 agaatcgcat acgcgataca tgccaccaga atcgagtcta ccgaagggat cgtcgtcagg
     1201 gtgaataagc cttttacggt cataaagcca accgctgcgc ctatgatcca tgctgcgaat
     1261 gtcagcactt taatctgtga ctcctgggct aacactgact cttgatagcc attacggcga
     1321 taaaggaaaa agtcagcgat ataaattccg gcaacgggtg gcgtggcgat acccaggaac
     1381 agcagaaacg gaataaacca cgccataata tccatactgc ctacgatggc ggaaaggata
     1441 cccagcgcca cggtaatctg ccatttggga aagcgggtaa gcagtgtgga aaccaccagc
     1501 gtgccctgga acatattgcc cgcgttaccg gtgacgcagg cgaaaatcaa caacagcgcc
     1561 gcaggcagaa ccgcaccgaa taccgccatc gcacccagta aagatccctg accactaatg
     1621 gcgctcggcg ttgcacccgc ccagtagagc agagggtagg caaccagaaa cgtcacgcct
     1681 gcggcaatca gcgcatgttt gcggttatgc acaaaactac cgaaatcagg cagtgtggca
     1741 accagcacaa taatggtgcc aacaacggtg gaaacggcga cacctgtatt catctccaca
     1801 atcgccgctg gtactgccac ttcggtatgg gttgccacat agaggatgta acacagcacc
     1861 agcgcgataa ccggcacggc aacctgggca atttttccca gaacggcaaa gccaaatgcc
     1921 gtagaggcga caaaaatgac acagccagcc gctactaaca gtgccagtgg aacttccagt
     1981 ccatgttgag ccagtaagtc atgtaccgaa tgaccaaaca tattggcggt aacggcaatc
     2041 cagccgaaca gacttatcgc catcaaaatg ttaatggcga tggccccttt ttcgccaaac
     2101 gaatatttca caatgccata cgttggcagg cgggcttttt ctcccacgct aatggtgatg
     2161 gctgaaagta aggtcagaat gacgccgcct aacaatacga cgctaattaa actgctgctg
     2221 gaaagttgat tgccgaggct tgaggatgac aacagtacag gtgtgacggc aatacccagc
     2281 aaaatcatgg caatacgcaa tcctgatgcc gtattcagat tctcttcttt tttacgcata
     2341 gtatgttccc tttataaatg cgcgttacac agtatgaaaa tgcccggtgt atttgcgcca
     2401 gctacccagt tctgtaattt cccgttggcc ttcggtcagc cagttttctg ccagtacgcc
     2461 cagcacttcg ctaccatcgg caagtttgat tttgccaatt gccagcccag cgggttcgct
     2521 cattaacaga gcggcaaaac tggccagcgg taattgccaa atttctacgt cgacgtgtgt
     2581 tcctccttca ttcacgcgaa tcattcccgg atggcgatca ttaatactcc acaaccggta
     2641 atgggcgtca gttcggtctt cacggacaaa aataccgccc gctttttgca tatttgggtt
     2701 gagttccagg ccgcgcatca gcgttccgtt aacggctaaa agcgttgtag tcattgtgtt
     2761 ctccagagaa ttaaagggtt atccatgtac cgctctgatg cgccagggct gcttttatgg
     2821 tttgcggtga agtaatcagg ccgctggcct gtttgccttg ttgctggctg gcattgatga
     2881 aatcaacaat ggcttccact ttgggttgca tactgccgac accaaactga ccttcccgca
     2941 acagcgtttg cgcttcggca acgctgatag cgtcgagcca ctgttgttgt ggtgtaccaa
     3001 agttaatcgc tactttttct acaccggtgg ggatcaccag cagatctgcg cctaactgac
     3061 tggcgagcag ggcggaggcc agatctttat cgatcacggc ttccacacct ttcagttgtt
     3121 gcccgtctcg caccaccgga ataccgccgc cgccgcaggc aataaccaga tatccctggc
     3181 gcaccaggtg agcgatggtg tcgtgctcaa taatttccag tggtgcagga gagggaactg
     3241 tacgccgcca accacgcccg gcgtcctcca tcagcgtcca gccgagttgt tgttggcgtt
     3301 gttgggccgt tgcttcatcg agaaacgcgc caattggttt gctggggctg gcgaaagcat
     3361 cgtcatgtgg gctgactcgc gtttatgtca ccagggcaat taccggtttg tttatgccac
     3421 ggcgagccaa ttcgttatgc agcgctttct ggaacatgta gccaattgca ccttgtgtat
     3481 cacccacggc gtaatcaagt ggaaccggag aaacttcgtt actggcgagt tcagagcggc
     3541 gcagaataaa gcccacctgc gggccattac cgtgggttag caccaggtcc catccggctt
     3601 caaccatatc aacgatatgt tgcacgcttt ccatcactgc aacatattga tcgggaatac
     3661 tattgcgttg ctcgtcctga atgagcgcat tgccgccaac cgcgacgaca gccagtggct
     3721 taaccatgat ggttctcctt tacgacgatc tccgccagtg cacgtacagc ggcgacaaaa
     3781 caggccatcg gcgcagtggt gatcccggca ccaatttgcc cgacccccgc ttgtttgtgg
     3841 gcgatacccg tattgatgac cggtaaaatg ccgcgatcgg tcactttacg cgcatcaatc
     3901 cctgctgcgg tgggggcaaa attgagggcc ggaagtgtaa atgcggggtt accgccgagg
     3961 gtgatggctt gcatccgacg gctgttattg gtggcatcgg caggggtgcc tcccacaaat
     4021 ttgacgatcg ccggagacga cgccatcgcg aagccaccca cacctgcggt ttcggtaatt
     4081 gcgctatcac ccaggtcagc agcagcgtcg tcaacgccaa atccggggaa gaacagacct
     4141 tcaacggcat tggcaggagc ctggaaccag cgatcgccag tactggaaag ccgaatacca
     4201 aaattcaccc cgttacgcgc catcaccgta accattgaac tgaacggtac attggcggcg
     4261 gcatccatca ttgctttaca ggcagccatc gacagattga ggaagaagtg atcgttaccg
     4321 gtgataaatg ccacgacgcg ctggatatgt tcctgtggaa gagagcaggc cagcaatgcc
     4381 gggagcaggc gcttaatcag caaaccggta gcggcagcat tgcggttatg aacttcatcc
     4441 cccatatgca gcgcctgcgc cattaatggc ttgagttcca gctcgccgtg ttgtgcgatt
     4501 gctgctttca ttgccggcgc cagttcatcg cgcatccagg ccagacgatt gagaacttca
     4561 tcgttattgg cgccaaaacg gagaacctta cccagacctt cattgaaatt gctaaaagta
     4621 cggtggccgt tggttttgtt ttctaccacc cataacggca tcgacgggct gatgatgcct
     4681 gccattgggc caaccgcgtg ataatgatga caaggggcaa ggtcgatttc gcctgcgttt
     4741 atttggtttt cagcgtcttg ctggctggtg gcccaacctt cgtacagcat ggcaccaata
     4801 atcgcccctt ttaccggacc acacatctct tcccaggcaa tcggcgggcc agagtgcaaa
     4861 atacgttttt gttcagccat tgctggaatg gcctctttag cgagcatgac atccaccagc
     4921 acgggctgtg cttcgagata acgtgtcatg gcgatttggt tggcattttc taccagggga
     4981 tgacgtagca acgatgccag atccaatcca gcgtcaatat cgccttgtgc tggtggctgc
     5041 cagttgagtg caattgcgct tcccccggca tgttgaatat tgtcagcaaa actgtgtagt
     5101 cccgcgttca caaccttcaa aggctgatta aataaggtat tcatgcgttt agcccctttt
     5161 tctgcaactg agtctgtgca acggtgctgg cccatagcgc cgcctgtgca ttgctgctgg
     5221 cgatgatgac ccctgcattt tgtagtgcgc ttatctgctg actgcgtatt tgcggatcag
     5281 cttccgttcc gcaaacgtgc gcaatcagca acggtacgtt attcatgtcg atgtgtgaaa
     5341 gttggtccag taattcgctg gcaggcgtcg tggaagcgcc ataaccaagt accaggtcga
     5401 ataacacgac ggcagtgtgt gagtcgttga gttcgttgag cagacgttga ttgcgcagag
     5461 tcgggtcgat cattggatgc ggtttgccac gggtgaaatc atcgtcgccc atatcgatca
     5521 acgtgtgatc ttcgctttgc cagatatttg ccagagcacg attacccgca actggcgtat
     5581 tggaagctgc gctgaatccc ttttgttggc agataagttg cgcttcgtag cagaaggttc
     5641 ctccggcgaa aacaccgcgt atcgcctggc gatgggctgg caggcgctgg caggcgtttt
     5701 gtaacattgt gagatcgtcg cagggtacat cggtttcgac agccggaagt ggctgaacat
     5761 tcagtaacgc cacagcgatg tcggcggcgc tggcaagtgt ggtggccgcg gtgatattgg
     5821 gacgtgccag atcatgcgga ttcgcaccga ggaaattcac caccacgggt tttccacaag
     5881 cctcggcgcg ctccagaata gtctgggcaa cgatcggtga aggcggtttt gaaatcagca
     5941 caatcacgcg agtttcatcg tcttgcgcca gcgcatctaa agcgaagagc atcgatattc
     6001 caccaatctc ttcactgaga tcatgtccac cggttcccaa cgcctgagaa atacccgctc
     6061 cgagctggtc gattcggcaa gtcacttcct gtagcccggt gcctgacgcg ccaataacac
     6121 caattgctcc gcgtttcacc acattagcga agcctaacgg aatgccgtta acaatggcgg
     6181 ttccacagtc aggtcccatt acgatgcgct gacgttctcg ggcaagcgtt ttgatggatt
     6241 tttcctgccc gatactgacg ttatcgctga acatcatcac gttcattccg aggtttaacg
     6301 ctttaattgc ttcggcggcg gcgtagtctc ccggtacaga aatgagtgcc aggttggctt
     6361 cgggttcggc ttccagtgcc atttccaggc tgaccttttc cggtgtttta atgccactat
     6421 ccgtttcatc atcgggtttg ctggtcagac gttgctgggc cagtgccagt gcttcattgc
     6481 aaatatcttc ttcacccatg actgcaatca ctaaatcatt gggactggcg ttgatttcgt
     6541 tacccagacc cgcatcgcgc aattgttcga gattattggg cgttcccatg acgaccgagg
     6601 cctgttgaat accgggcaac ttgctgattt gcgcggaaat ctgcatcaga gaaactgagt
     6661 cctgataaag attactgaaa actttatatt taactttcat gctgttacgc ctgtttcatt
     6721 ccgcccgata gcaaaattcg ggcaaagcat ccccgtgtag ccccataaac gggctacggg
     6781 agaaaaggtt gaaatcaatt catggtgttc agggttcgaa agccatgcag cattcctgca
     6841 aggcaatcta ctcctgacgt tgcgccaaat tgcatgacct gttctgcaca ggatgcgatt
     6901 gtcattgccg atgcgctccc aaccagttgt gcgagtaact ggcaaatcgg ttctgaaaaa
     6961 tatccctgaa gcgcacgctc cagataatga cgactgatat ccgtggtgtt gtgcgcatgt
     7021 tgatcaattg cctgctgcaa ccggtattga tgatcggcaa gtggtgcagg gagttgccat
     7081 aaccagagcg ctgccagata gcccaacaaa tagtcatctc catcgggcgt taagccttta
     7141 ccaaagccga tcagttgtgg cacctggcgt tccagcgcat ctggatgttc cataattgct
     7201 aatccctggt gcgtgaggct atccggcagg tgcaatgtgc tttgaacgtt atgttccaga
     7261 caaaataaaa gccgttgcga tgccatcgtt gggtagtgct ggaaaaccaa agggaatgcc
     7321 tcaaatgtca gcattctccc cacaaatggc gactgccaga gcgttgcgtt atcgagcgcc
     7381 acacaaaatc gaggtgtccg cagcaagcct ggggttaact gaatgggatc tgaatgggca
     7441 atctctcttc gccagtccca acattcaggt agccagactc tggcagcatc agggagattc
     7501 agatacgttt gcgcacacaa cagagtgatg aatgttcccg cgtctgaata cagacttacc
     7561 gcatgtttaa aagcgctatg cagacgtaaa tcttgcgttt cattctgcgg gaaaagatat
     7621 cccaacgacg atgcgcaaag cgtaacctgt accatcggcg ttagccttcc agacctgcaa
     7681 taatggcttt ggaatcagta acccaaccga aaatggcacc ttgtgctttg atcatctcga
     7741 gggcgtattt ctggaattcc gggaaatagg agccgacaca atcttccggg ataatgcatt
     7801 catagccacg atcatttgct tcgcgcacgg tggtggttac gcaaacttcg gttgttacac
     7861 cacaaactat cagcgtcttg ataccgtggt tttgcaaaat cagatgtaag tcggtttgat
     7921 aaaacgcgcc tttgccaggc ttatcaatca ccggttcgcc tgccaccggg tacagttcag
     7981 gaatgatgtc atgacctgct tcacctctga ccagaatgcg tcccatcggt cctggttcac
     8041 caataaaggt ttttccgccg cgcgtcagtt tggctggcgg gcagtcgctt aagtctgcac
     8101 gatggccttc acgagtatga ataaccataa ttcctttttg acgtgcggct gccagcacct
     8161 ctgtacatgg tgcgatagcc gtgcgtacca gtgaaacatc attgcccagt gcctcaccga
     8221 agccccctgc ttcgacaaag tcacgctgca tgtcgatcat gaccagtgca gttgtgcgcg
     8281 gatcaaaagg taattcaaag ggttgagcct gaaaaatgga ttgcgtcatg gtaaatctcc
     8341 tgtctgatgt ttaaacgatc cagttgttct gagaacgagt attcaggaga aaagcggcac
     8401 aaaacaggaa aaaagtttgc cgcacagcaa cgaaattttt gcattcagca ggtggtgtga
     8461 tgacgaatgt gaagggagtc aaagagtggc atacagcaac aaattttttg atgctgtatg
     8521 gttttgagat taaaaaaatc gttgaaattg tgtgagttta atcatcagat ttagcgacta
     8581 tggcgttact ttttaacacc atactgtgta aaccattcca acattcgctg ccagccgtct
     8641 ttcgcagatt cggcatgata gctcgggcga taatcggcgt tgaacgcatg ccctgcatcc
     8701 gggtacacga taatctctgc tttcgcgttt gctgcccgca gcgcccggcg catcgtttcc
     8761 acgctctctt gcggaatgct gttatcctgg ccgccatata agccgagaac cggcgcgtta
     8821 agatcggttg cgatatcaac aggttgtttc ggtgaattca gcgacttatc gcccgtcagt
     8881 ttgccgtacc acgccactgc ggcttttagc tgtggattat gcgcggcata cagccaggtg
     8941 atacgtccac cccagcagaa tccggtgatc attaaacgat gaacatcgcc gccgttgcgg
     9001 gaagcccaac tggcgacatg atcgagatcg gccagcacct gcgagtcagg cacttttgct
     9061 accagaccgc taagcaacgt ggggatatcg gcaaaatcat tcggatcgcc ttcgcggaag
     9121 taaagttcag gtgcgatagc cagatacccc tccagcgcca gacggcgaca aatgtcgcgg
     9181 atatgttcat gcacgccaaa aatttcctgc actacaatga ccactggcag taggccatcg
     9241 ctttgctttg gtctggcatg gtaagcaggc atgttatccc cttgtgaagg gatagaggtg
     9301 aagcccgcca caatcgcgtc gtccggagtc tgaacgatgg tcgaagcgag aggcgatgca
     9361 gcaggtgcaa atccagattg ttgtgttgtt gccatggtat tctccgtacc cttataaaaa
     9421 tgttgcgcaa tgttaactat agtcagcatg caacaaatca cattgcctga atcggctcat
     9481 cttttatgca gtcctgcaga atgaagagtg atttatgtga tttacatcac ttttggtggg
     9541 taaatttatg caacgcattt tcgtcatggt gatgagtatc acgaaaaaat gttaaaccct
     9601 tcggtaaagt gtctttttgc ttcttctgac taaaccgatt cacagaggag ttgtatatgt
     9661 ccaagtctga tgtttttcat ctcggcctca ctaaaaacga tttacaaggg gctacgcttg
     9721 ccatcgtccc tggcgacccg gatcgtgtgg aaaagatcgc cgcgctgatg gataagccgg
     9781 ttaagctggc atctcaccgc gaattcacca cctggcgtgc agagctggat ggtaaacctg
     9841 ttatcgtctg ctctaccggt atcggcggcc cgtctacctc tattgctgtt gaagagctgg
     9901 cacagctggg cattcgcacc ttcctgcgta tcggtacaac gggcgctatt cagccgcata
     9961 ttaatgtggg tgatgtcctg gttaccacgg cgtctgttcg tctggatggc gcgagcctgc
    10021 acttcgcacc gctggaattc ccggctgtcg ctgatttcga atgtacgact gcgctggttg
    10081 aagctgcgaa atccattggc gcgacgactc acgttggcgt gacagcttct tctgatacct
    10141 tctacccagg tcaggaacgt tacgatactt actctggtcg cgtagttcgt cactttaaag
    10201 gttctatgga agagtggcag gcgatgggcg taatgaacta tgaaatggaa tctgcaaccc
    10261 tgctgaccat gtgtgcaagt cagggcctgc gtgccggtat ggtagcgggg gttatcgtta
    10321 accgcaccca gcaagagatc ccgaatgctg agacgatgaa acaaaccgaa agccatgcgg
    10381 tgaaaatcgt ggtagaagcg gcgcgtcgtc tgctgtaatt atcttctctt gtctgaaggc
    10441 cgacgcgttc ggccttttgt atttttgcgt agcgcctcgc aggaaatgcc tttccaactg
    10501 gacgtttgta cagcacaatt ctattttgtg cgggtaagtt gttgcgtcag gaggcgttgt
    10561 ggatttctca atcatggttt acgcagttat tgcattggtg ggtgtggcaa ttggctggct
    10621 gtttgccagt tatcaacatg cgcagcaaaa agccgagcaa ttagctgaac gtgaagagat
    10681 ggtcgcggag ttaagcgcgg caaaacaaca aattactcaa agcgagcact ggcgcgctga
    10741 gtgcgagtta ctcaataacg aagtgcgcag cctgcaaagt attaacacct ctctggaggc
    10801 cgatctgcgt gaagtgacca cgcggatgga agctgcacag caacatgctg acgataaaat
    10861 tcgccagatg attaacagcg agcagcgcct cagtgagcag tttgaaaacc tcgccaaccg
    10921 tatttttgag cacagcaatc gccgggttga tgagcaaaac cgtcagagtc tgaacagcct
    10981 gttgtcgccg ctacgtgaac aactggacgg tttccgccgt caggttcagg acagcttcgg
    11041 taaagaagca caggaacgtc ataccctgac ccacgaaatt cgcaatctcc agcagctcaa
    11101 cgcgcaaatg gctcaggagg cgatcaacct gacgcgcgcg ctgaaaggcg acaataaaac
    11161 ccagggcaac tggggcgagg tggtattgac gcgggtgctg gaggcttccg gtctgcgtga
    11221 agggtatgaa tatgaaactc aggtcagcat cgaaaatgac gcccgctcgc ggatgcagcc
    11281 ggatgtcatc gtgcggttgc cgcagggaaa agatgtggtg atcgacgcca aaatgacgct
    11341 ggtcgcctat gaacgctatt tcaacgccga agatgactac acccgcgaaa gcgcgctaca
    11401 ggaacatatc gcgtcggtgc gtaaccatat ccgtttgctg ggacgcaaag attatcaaca
    11461 gctgccgggg ctaagaaccc tggattacgt gctgatgttt attcccgttg aacccgcttt
    11521 tttactggcg cttgaccgcc agccggagct gatcaccgaa gcgttgaaaa acaatatcat
    11581 gctggttagc ccgactacgc tgctggtggc gctgcgcact atcgccaacc tgtggcgtta
    11641 tgagcatcaa agccgcaacg cccagcaaat cgccgatcgt gccagcaagc tgtacgacaa
    11701 gatgcgtttg ttcgtcgatg acatgtcagc gattggtcaa agtctcgaca aagcgcagga
    11761 taactaccgc caggcaatga aaaaactctc ttcaggtcgc ggaaatgtgc tggcgcaggc
    11821 agaagcgttt cgcggtttag gggtagaaat taaacgcgag attaatcctg atttggctga
    11881 acaggcggtg agccaggatg aagagtatcg actgcggtcg gttccggagc agccgaatga
    11941 tgaagcttat caacgcgatg atgaatataa tcagcagtcg cgctagccca tttggggtag
    12001 ttaagccggg tagaaatcta gggcatcgac gcccaatctg ttacacttct ggaacaattt
    12061 tttgatgagc aggcattgag atggtggata agtcacaaga aacgacgcac tttggttttc
    12121 agaccgtcgc gaaggaacaa aaagcggata tggtcgccca cgttttccat tccgtggcat
    12181 caaaatacga tgtcatgaat gatttgatgt cattcggtat tcatcgtttg tggaagcgat
    12241 tcacgattga ttgcagcggc gtacgccgtg ggcagaccgt gctggatctg gctggtggca
    12301 ccggcgacct gacagcgaaa ttctctcgcc tggtcggaga aactggcaaa gtggtccttg
    12361 ctgatatcaa tgaatccatg ctcaaaatgg gccgcgagaa gctgcgtaat atcggtgtga
    12421 ttggcaacgt tgagtatgtt caggcgaacg ctgaggcgct gccgttcccg gataacacct
    12481 ttgattgcat caccatttcg tttggtctgc gtaacgtcac cgacaaagat aaagcactgc
    12541 gttcaatgta tcgcgtgctg aaacccggcg gccgcctgct ggtgcttgag ttctcgaagc
    12601 caattatcga gccgctgagc aaagcctatg atgcatactc cttccatgtg ctgccgcgta
    12661 ttggctcact ggtcgcgaac gacgccgaca gctaccgtta tctggcagaa tccatccgta
    12721 tgcatcccga tcaggatacc ctgaaaacca tgatgcagga tgccggattc gaaagtgtcg
    12781 actactacaa tctgacggca ggggttgtgg cgctgcatcg tggttataag ttctgacagg
    12841 agaccggaaa tgccttttaa acctttagtg acggcaggaa ttgaaagtct gctcaacacc
    12901 ttcctgtatc gctcacccgc gctgaaaacg gcccgctcgc gtctgttggg caaagtgctg
    12961 cgcgtggagg taaaaggctt ttcgacgtca ttgattctgg tgttcagcga acgccaggtc
    13021 gatgtactgg gcgaatgggc aggcgatgct gactgcaccg ttatcgctta cgccagtgtg
    13081 ttgccgaaac ttcgcgatcg ccagcagctt gccgcactga ttcgcagtgg tgagctggaa
    13141 gtgcagggtg atattcaggt ggtgcaaaac ttcgttgcgc tggcagatct ggcagagttc
    13201 gaccctgcgg aactgctggc cccttatacc ggtgatatcg ccgctgaagg tatcagcaaa
    13261 gccctgcgcg gaggcgcaaa gttcctgcat cacggcatta agcgccagca acgttatgtg
    13321 gcggaagcca ttactgaaga gtggcgtatg gcacccggtc cgcttgaagt ggcctggttt
    13381 gcggaagaga cggctgccgt cgagcgtgct gttgatgccc tgaccaaacg gctggaaaaa
    13441 ctggaggcta aatgacgcca ggtgaagtac ggcgcctata tttcatcatt cgcacttttt
    13501 taagctacgg acttgatgaa ctgatcccca aaatgcgtat caccctgccg ctacggctat
    13561 ggcgatactc attattctgg atgccaaatc ggcataaaga caaaccttta ggtgagcgac
    13621 tacgactggc cctgcaagaa ctgggaccgg tatggatcaa gttcgggcag atgttatcaa
    13681 cccgccgcga tctttttccg ccgcatattg ccgatcagct ggcgttattg caggacaaag
    13741 tcgctccgtt tgatggcaag ctggcgaagc agcagattga agctgcaatg ggcggcttgc
    13801 cggtagaagc gtggtttgac gattttgaaa tcaagccgct ggcttctgct tctatcgccc
    13861 aggttcatac cgcgcgattg aaatcgaatg gtaaagaggt ggtgattaaa gttatccgcc
    13921 cggatatttt gccggtcatt aaagcggatc tgaaacttat ttaccgtctg gctcgctggg
    13981 tgccgcgttt gctgccggat ggtcgccgtc tgcgcccaac cgaagtggtg cgcgagtacg
    14041 aaaagacctt gattgatgaa ctgaatttgc tgcgggaatc tgccaacgcc attcagcttc
    14101 gacgcaattt tgaagacagc ccgatgctct acatcccgga agtttaccct gactattgta
    14161 gtgaagggat gatggtgatg gagcgtattt acggcattcc ggtgtctgat gttgcgacgc
    14221 tggagaaaaa cggcaccaac atgaaattgc tggcggaacg cggcgtgcag gtgttcttca
    14281 ctcaggtctt tcgcgacagc tttttccatg ctgatatgca ccctggcaac atcttcgtaa
    14341 gctatgaaca cccggaaaac ccgaaatata tcggcattga ttgcgggatt gttggctcgc
    14401 taaacaaaga agataaacgc tatctggcgg aaaactttat cgccttcttt aatcgcgact
    14461 atcgcaaagt ggcagagcta cacgtcgatt ctggttgggt gccaccagat accaacgttg
    14521 aagagttcga atttgccatt cgtacggtct gtgaacctat ctttgagaaa ccgctggccg
    14581 aaatttcgtt tggacatgta ctgttaaatc tgtttaatac ggcgcgtcgc ttcaatatgg
    14641 aagtgcagcc gcaactggtg ttactccaga aaaccctgct ctacgtcgaa ggggtaggac
    14701 gccagcttta tccgcaactc gatttatgga aaacggcgaa gcctttcctg gagtcgtgga
    14761 ttaaagatca ggtcggtatt cctgcgctgg tgagagcatt taaagaaaaa gcgccgttct
    14821 gggtcgaaaa aatgccagaa ctgcccgaac tggtttacga cagtttgcgc cagggcaagt
    14881 atttacagca tagtgttgat aagattgccc gcgagcttca gtcaaatcat gtacgtcagg
    14941 gacaatcgcg ttattttctc ggaattggcg ctacgttagt attaagtggc acattcttgt
    15001 tggtcagccg acctgaatgg gggttgatgc ccggctggtt aatggcaggt ggtctgatcg
    15061 cctggtttgt cggttggcgc aaaacacgct gattttttca tcgctcaagg cgggccgtgt
    15121 aacgtataat gcggctttgt ttaatcatca tctaccacag aggaacatgt atgggtggta
    15181 tcagtatttg gcagttattg attattgccg tcatcgttgt actgcttttt ggcaccaaaa
    15241 agctcggctc catcggttcc gatcttggtg cgtcgatcaa aggctttaaa aaagcaatga
    15301 gcgatgatga accaaagcag gataaaacca gccaggatgc tgattttact gcgaaaacta
    15361 tcgccgataa gcaggcggat acgaatcagg aacaggctaa aacagaagac gcgaagcgcc
    15421 acgataaaga gcaggtgtaa tctgtgtttg atatcggttt tagcgaactg ctattggtgt
    15481 tcatcatcgg cctcgtcgtt ctggggccgc aacgactgcc tgtggcggta aaaacggtag
    15541 cgggctggat tcgcgcgttg cgttcactgg cgacaacggt gcagaacgaa ctgacccagg
    15601 agttaaaact ccaggagttt caggacagtc tgaaaaaggt tgaaaaggcg agcctcacta
    15661 acctgacgcc cgaactgaaa gcgtcgatgg atgaattacg ccaggctgcg gagtcgatga
    15721 agcgttccta cgttgcaaac gatcctgaaa aggcgagcga tgaagcgcac accatccata
    15781 acccggtggt gaaagacaat gaaactgcgc atgaaggcgt aacgcctgct gctgcacaaa
    15841 cgcaggccag ttcgccggaa cagaagccag aaactacgcc agagccggtg gtaaaacctg
    15901 ctgcggacgc tgaaccgaaa accgctgcac cttccccttc gtcgagtgat aaaccgtaaa
    15961 catgtctgta gaagatactc aaccgcttat cacgcatctg attgagctgc gtaagcgtct
    16021 gctgaactgc attatctcgg tgatcgtgat attcctgtgt ctggtctatt tcgccaatga
    16081 catctatcac ctggtatccg cgccattgat caagcagttg ccgcaaggtt caacgatgat
    16141 cgccaccgac gtggcctcgc cgttctttac gccgatcaag ctgaccttta tggtgtcgct
    16201 gattctgtca gcgccggtga ttctctatca ggtgtgggcg tttatcgccc cagcgctgta
    16261 taagcatgaa cgtcgcctgg tggtgccgct gctggtttcc agctctctgc tgttttatat
    16321 cggcatggcg ttcgcctact ttgtggtctt tccgctggca tttggcttcc ttgccaatac
    16381 cgcgccggaa ggggtacagg tatccaccga catcgcgagc tatttaagct tcgttatggc
    16441 gctgtttatg gcgtttggtg tctcctttga agtgccggtg gcaattgtgc tgctgtgctg
    16501 gatggggatt acctcgccag aagacttacg caaaaaacgc ccgtatgtgc tggttggtgc
    16561 attcgttgtc gggatgttgc tgacgccgcc ggatgtcttc tcgcaaacgc tgttggcgat
    16621 ccctatgtac tgcctgtttg aaatcggtgt cttcttctca cgcttttacg ttggtaaagg
    16681 gcgaaaccgg gaagaggaaa acgacgctga agcagaaagc gaaaaaactg aagaataaat
    16741 tcaaccgccc gtcagggcgg ttgtcatatg gagtacagga tgtttgatat cggcgttaat
    16801 ttgaccagct cgcaatttgc gaaagaccgt gatgatgttg tagcgcgcgc ttttgacgcg
    16861 ggagttaatg ggctactcat caccggtacc aatctgcgtg aaagccagca ggcgcaaaag
    16921 ctggcgcgtc agtattcgtc ctgttggtca acggcgggcg tacatcctca cgacagcagc
    16981 cagtggcaag ctgtgactga agaagcgatt attgagctgg ccgcgcagcc agaagtggtg
    17041 gcgattggtg aatgtggtct cgactttaac cgcaactttt cgacgccgga agagcaggaa
    17101 ctcgcttttg ttgcccagct acgcattgcc gcagaattaa acatgccggt atttatgcac
    17161 tgtcgcgatg cccacgagcg gtttatgaca ttgctggagc cgtggctgga taaactgcct
    17221 ggtgcggttc ttcattgctt taccggcaca cgcgaagaga tgcaggcgtg cgtggcgcgt
    17281 ggaatttata tcggcattac cggttgggtt tgcgatgaac gacgcgggct ggagctgcgg
    17341 gaattgttgc cgttgattcc ggcggagaaa ttgctgatcg aaactgatgc gccgtatctg
    17401 ctccctcgcg atctcacgcc aaagccatca tcccggcgca acgagccagc ccatctgccc
    17461 catattttgc aacgtattgc gcactggcgt ggagaagatg ccgcatggct ggctgccacc
    17521 acggatgcta atgtcaaaac actgtttggg attgcgtttt agagtttgcg gaactcggtg
    17581 ttcttcacac tgtgcttaat ctctttatta ataagattaa gcaatagcat ggagcgagcc
    17641 tcgccgtctg gctcggtgaa aatggcctga aaaccttcga atgcgccttc agtaataatc
    17701 actttatcac caggatacgg cgtcgatgga tcgacaatat ctttcggttt atacactgat
    17761 aactgatgaa tcaccgccga tgggactatc gctggtgacg cgccaaagcg cacgaagtgg
    17821 ctgacaccgc gggtcgcgtt gatagtcgtg gtatgaatca cttccggatc gaactccaca
    17881 aacaagtagt tggggaacaa tggctcactg actgcagtac gtttgccacg cacgattttt
    17941 tccagggtga tcatcggtgc caggcaattc acagcctgtc tttcgaggtg ttcctgggca
    18001 cgttgaagtt gcccgcgctt gcagtacagt aaataccagg attgcataat gactcttatc
    18061 cgtttaatcg gggcgcaagg atagcaaaag ctttacgcta agttaattat attccccggt
    18121 ttgcgttata ccgtcagagt tcacgctaat ttaacaaatt tacagcatcg caaagatgaa
    18181 cgccgtataa tgggcgcaga ttaagaggct acaatggacg ccatgaaata taacgattta
    18241 cgcgacttct tgacgctgct tgaacagcag ggtgagctaa aacgtatcac gctcccggtg
    18301 gatccgcacc tggaaatcac tgaaattgct gaccgcactt tgcgtgccgg tgggcctgcg
    18361 ctgttgttcg aaaaccctaa aggctactca atgccggtgc tgtgcaacct gttcggtacg
    18421 ccaaagcgcg tggcgatggg catggggcag gaagatgttt cggcgctgcg tgaagttggt
    18481 aaattattgg cgtttctgaa agagccggag ccgccaaaag gtttccgtga cctgtttgat
    18541 aaactgccgc agtttaagca agtattgaac atgccgacaa agcgactgcg tggtgcaccc
    18601 tgccaacaaa aaatcgtctc tggcgatgac gtcgatctca atcgcattcc cattatgacc
    18661 tgctggccgg aagatgccgc gccgctgatt acctgggggc tcaccgtaac gcgcggcccg
    18721 cataaagagc ggcagaatct gggcatttat cgccagcagc taattggtaa aaacaaactg
    18781 attatgcgct ggctgtcgca tcgcggcggc gcgttggatt atcaggagtg gtgtgcggcg
    18841 catccgggcg aacgtttccc ggtttctgtg gcgctgggtg ccgatcctgc cacgattctc
    18901 ggtgcagtca cccccgttcc ggatacgctt tcagagtatg cgtttgccgg attgctgcgc
    18961 ggcaccaaga ccgaagtggt gaagtgtatc tccaatgacc ttgaagtgcc cgccagtgcg
    19021 gagattgtgc tggaagggta tatcgaacaa ggcgaaactg cgccggaagg gccgtatggc
    19081 gaccacaccg gttactataa cgaagtcgat agtttcccgg tatttaccgt gacgcatatt
    19141 acccagcgtg aagatgcgat ttatcattcc acctataccg ggcgtccgcc agatgaacct
    19201 gcggtactgg gtgtagcact gaacgaagtg ttcgtgccga ttctgcaaaa acagttcccg
    19261 gaaattgtcg atttttacct gccgccggaa ggctgttctt atcgtctggc ggtagtgacg
    19321 atcaaaaaac agtacgccgg acacgcgaag cgcgtcatga tgggcgtctg gtcgttctta
    19381 cgccagttta tgtacactaa atttgtgatc gtttgcgatg atgacgtcaa cgcacgcgac
    19441 tggaacgatg tgatttgggc gattaccacc cgtatggacc cggcgcggga tactgttctg
    19501 gtagaaaata cgcctattga ttatctggat tttgcctcgc ctgtctccgg gctgggttca
    19561 aaaatggggc tggatgccac gaataaatgg ccgggggaaa cccagcgtga atggggacgt
    19621 cccatcaaaa aagatccaga tgttgtcgcg catattgacg ccatttggga tgaactggct
    19681 atttttaaca acggtaaaag cgcctgatgc gcgtttgttt tgccctattt atcgatccga
    19741 cagagaaagc gcatgacaac cttaagctgt aaagtgacct cggtagaagc tatcacggat
    19801 accgtatatc gtgtccgcat cgtgccagac gcggcctttt cttttcgtgc tggtcagtat
    19861 ttgatggtag tgatggatga gcgcgacaaa cgtccgttct caatggcttc gacgccagat
    19921 gaaaaagggt ttatcgagct gcatattggc gcttctgaaa tcaaccttta cgcgaaagca
    19981 gtcatggacc gcatcctcaa agatcatcaa atcgtggtcg atattcccca cggagaagcg
    20041 tggctgcgcg atgatgaaga gcgtccgatg attttgattg cgggcggcac cgggttctct
    20101 tatgcccgct cgattttgct gacagcgttg gcgcgtaacc caaaccgtga tatcaccatt
    20161 tactggggcg ggcgtgaaga gcagcatctg tatgatctct gcgagcttga ggcgctttcg
    20221 ttgaagcatc ctggtctaca agtggtgccg gtggttgaac aaccggaagc gggctggcgt
    20281 gggcgtactg gcaccgtgtt aacggcggta ttgcaggatc atggtacgct ggcagagcat
    20341 gatatctata ttgccggacg ttttgagatg gcgaaaattg cccgtgacct gttttgcagc
    20401 gagcgtaatg cgcgggaaga tcgcctgttt ggcgatgcgt ttgcatttat ctgagatata
    20461 aaaaaacccg cccctgacag gcgggaagaa cggcaactaa aactttattc agtggcatta
    20521 ctctacgttg catccggcaa acagtgcccg atgcaacgct gccgcgtctt atcgggccta
    20581 caaaagttct gaaccgtggg ccggataagg cgttcacgcc gtatccggca tacagtgccc
    20641 gatgcgacgc tgccgcgtct tatcgggcct acaaaagttc tgaaccgtag accggataag
    20701 gcgttcacgc cgcatccggc aagtggttaa acccgctcaa acaccgttgc aatgccctga
    20761 cccagaccga tacacatcgt cgccagacca aactggacgt ctttgtgttc catcagattc
    20821 agcagcgtgg tgctgatacg cgcaccggaa caacccagcg gatgacccag cgcgatcgcg
    20881 ccaccgttga ggttgatctt ctcgtcaatc tgctccatta gcccgagatc tttaatacac
    20941 ggcaggatct gcgcggcaaa ggcttcgttc atctcgaata cgccgatatc gctggcagaa
    21001 agccccgctt ttttcagcgc cagcttcgag gccggaaccg gaccataacc cataatagat
    21061 gggtcacaac ccacaaccgc catcgaacgc acgcgggcgc gcggcttaag acctaattca
    21121 cgggcgcggc tttcactcat caccagcatg gcagctgcgc catcggaaag cgcggaagat
    21181 gtacccgccg ttaccgtacc gctgacggga tcaaacgccg gacgcagtgt ggcgagggct
    21241 tccacggtgg tttccgggcg aatcacttcg tcgtaattaa actgcttcaa tacgccgtcg
    21301 gcatcgtgac caccggtcgg gatgatttca tttttaaatg cgcctgactg cgtggcggcc
    21361 cacgcgcggg cgtgtgaccg cgcggcaaag gcatcctgca tttcacggct gataccatgc
    21421 atacgcgcca gcatttctgc cgttaagccc atcatgcccg ccgctttggc gacattacgg
    21481 ctaaggccgg ggtgaaaatc gacgccgtga ctcattggca catggcccat atgctccacg
    21541 ccgccaacca gacatgcctg cgcatcgccg gtcatgatca ttcgtgctgc gtcatgcagt
    21601 gcctgcatgg atgaaccaca caagcgatta acggtaaccg ccgggacaga gtgcggcact
    21661 tcagccaaca gtgccgcgtt acgggcgatg ttaaaaccct gctccagtgt ttgctgcaca
    21721 caaccccagt aaatatcgtc gagggccgct gcttccagcg ccgggttacg cgccagcagg
    21781 ctacgcatta aatgagcgga gagatcttcc gcacgcacgt gacgaaaagc accgcccttc
    21841 gaacggccca tcggggtgcg aattgcatcg acaatgacaa cctgttccat tgtgactcct
    21901 taagccgttt tcaggtcgcc aaccggacgg gctggctcaa ccgggggata gtacggttcg
    21961 ttatgacgtg ctttattacg cagaccttcc ggtacttcat acagcgggcc gaggtgctga
    22021 tattgctgtg ccatatcgag gtattttgcg ctgccgaggg tgtccagcca gcggaacgcg
    22081 ccgccgtgga acggagggaa gcccaggcca tagaccagcg ccatatccgc ttctgccgga
    22141 gtggcgataa tgccctcttc cagacagcgg accacttcgt tgaccatcgg gatcatcatg
    22201 cgggcgataa tctcttcttc gctgaaatcg cgcttcgcgt ggctgacctc tgccagcagg
    22261 tcgtcaacga cggcgtcttc ttctttcttc ggcttgcctt tactgtcttc tttataacgc
    22321 cagaaaccga ggccgttctt ctgaccaaaa cggttggcat caaacagcgc atcgatggca
    22381 tcacggtaat ctttctgcat ccgctgcggg aagcctgctg ccatcacggc ctgggcgtgg
    22441 tgcgcggtat caatccccac gacatccagc agatatgccg gacccatcgg ccagccaaac
    22501 tgtttttcca tcactttgtc gatcttgcgg aaatccgcgc cgtcgcgcag caactggctg
    22561 aatccggcga agtaggggaa cagtacacgg ttaacgaaga agccggggca gtcgttgacc
    22621 acaatcggcg tcttgcccat cttgctcgcc catgcgacga ctttcgcgat ggtttcgtcg
    22681 gagcttttct cgccgcgaat aatttccacc aacggcatcc ggtgcaccgg gttaaagaag
    22741 tgcatcccgc agaagttttc cggacgttcc agcgcgttgg ccagttcgct gataggaatg
    22801 gttgaagtgt tagacgccag cacggtatcc gggcgtactt tctgctcggt ttctgccagc
    22861 acggcttttt tcactttcgg gttttcaaca accgcttcta ccacaacatc cacgcggtca
    22921 aatccggcgt agtcgagcgt tgggtggatt gtggagatca cgccagccag tttcagacca
    22981 tcgatcttgc cgcgttcaag ctgcttgttc agcagtttcg cggcttcggt catgccgagg
    23041 gttaacgact tgtcgttgat atctttcatg ataaccggca cacctttcca cgcagactgg
    23101 taagcgatgc cgccgcccat aatgcctgcg cccagcacag cggcctgttt cggcgtttca
    23161 acgtctttgg tgagtttctt cgctttgcct tttacatatt gatcgttaag gaatatgccg
    23221 accagtgcgc gagcttcgtt ggtatgcgcc agcgggacaa aacttttgtt ttccaggttt
    23281 agggcttctt cacgaccaaa acgggccgca gcttcaatgg tttttactgc agtgatgggg
    23341 gccggataat gtttccccgc cgtttgcgcg accatccctt tagcgatggt gaagctcatg
    23401 gtggcttcaa tcttgctcag ttttagcggt tccagcttcg gctgacgttt tgctttccag
    23461 tcgaggtcgc cgttaatggc ctgacgtaaa atcgccttcg cgccttcaac cagtttttct
    23521 gctttgacta cgccatccac cagaccgatt ttcagcgcct gatccgcgcc gacatcttta
    23581 ccggcggcaa tgatttccag agcactgtca gcgcccagca tacgtggcat acgtacagaa
    23641 ccgccaaagc ccggcatgat gcccagtttg gtttccggca gaccgatgcg cagatccggc
    23701 gtcgccagac gataatcggt cgccagcacg cattcgcagc caccgcccag cgcatagccg
    23761 ttgacggcag caatggtcgg caccggcaga tcttccaggc gattaaacac gctattggca
    23821 aagtgcagcc actgacttaa ctgttcttca ggaacgagga acagggacaa aaattcggtg
    23881 atatcagcac cgacgataaa ggctgctttg ttcgaacgta gcagcagtcc ttttagatct
    23941 gattgctgtt ccagcacgcc gatggcctcg ccgaggctgg cgacggtcgc agtgtcgagt
    24001 ttattaactg aacctggggt atcaaatacc agttcggcaa tgccatcttc cagccagtca
    24061 aggtaaaggg tgtcgccttt gtaaagcatg tcagtctcct gaatccgcaa ggtgatctgg
    24121 tcgtaccaga tgcatcgaag tgtgtatttt gtgttaaaaa tatgcaaata aaatattaag
    24181 gaaatgccga tctgatcacg ctcggcagaa atcacgctct ggatgaacga tgtgctaaga
    24241 tgcggagact taaggtcaaa aaaacagaag ggtaaaaaat ggaatcactg gcctcgctct
    24301 ataaaaatca tatagctacc ttacaggaac ggactcgcga tgcgctggcg cgcttcaagc
    24361 tggatgcgtt acttattcac tccggcgaac tgttcaacgt ttttctcgac gatcatccct
    24421 atccgtttaa agtgaacccg caattcaaag cgtgggtgcc ggtaactcag gtgccaaact
    24481 gctggctgct ggtggatggc gtgaataagc cgaaactgtg gttttatctg ccggttgatt
    24541 actggcacaa cgtcgaaccg ctgccgaact ccttctggac tgaagatgta gaagtgatcg
    24601 cgctgccaaa agccgatggc attggtagtc tgttgcctgc tgcgcgcggc aatatcggtt
    24661 atatcggtcc ggtgccagaa cgtgcgctgc aactgggtat tgaggccagc aatatcaacc
    24721 cgaaaggggt gatcgactac ctgcattact atcgctcctt caaaaccgag tacgaactgg
    24781 cctgtatgcg tgaagcgcag aaaatggcgg tcaacggtca tcgcgcggca gaagaagcgt
    24841 tccgttctgg catgagcgag ttcgatatca acattgccta tctgaccgcg accggtcatc
    24901 gtgataccga cgtaccttat agcaacattg tggcgcttaa cgaacacgct gcggtgttgc
    24961 attacaccaa actggaccac caggcaccgg aagagatgcg cagctttctg ctggatgccg
    25021 gggccgaata taacggctat gcggctgatc taacccgtac ctggtcggca aaaagcgaca
    25081 acgactacgc acagctggtg aaagacgtaa atgatgagca actggcgctg atcgccacca
    25141 tgaaagcagg cgtcagctat gtggattacc atatccagtt ccatcagcgc atcgccaaac
    25201 tgctgcgtaa acatcaaatc atcaccgata tgagtgaaga agcgatggtc gaaaacgatc
    25261 tcaccggacc gtttatgccg cacggtatcg gccatccgct gggcctgcag gtgcatgacg
    25321 tagccggttt tatgcaggat gatagcggta cgcacctcgc ggcaccggca aaatatccgt
    25381 acctgcgctg cacccgtatt ctccagccgg gcatggtgtt aaccatcgaa ccgggtatct
    25441 acttcatcga atcgcttctg gcaccgtggc gtgaagggca gttcagcaaa cacttcaact
    25501 ggcagaaaat tgaagcactg aaaccgttcg gcggcattcg tatcgaagac aacgtggtga
    25561 ttcacgaaaa caacgtggaa aacatgaccc gggatctgaa actggcgtga tggaaagctg
    25621 gttaattcct gcggcaccgg tcacggtcgt tgaagagatc aaaaagagcc gcttcattac
    25681 gctgttggcg cataccgatg gcgttgaggc ggcaaaagcg tttgttgaat cggtgcgggc
    25741 agaacacccc gatgcccggc accattgcgt ggcgtgggtg gcgggtgcgc cggatgattc
    25801 tcaacagctg ggtttctctg acgacgggga gccggcggga acggcaggta aaccgatgct
    25861 cgcccaacta atgggcagcg gcgtcgggga aattaccgct gtggtggtgc gctactacgg
    25921 cggcatattg cttggcaccg gtgggttagt gaaagcgtat ggcggcggcg tgaatcaggc
    25981 gctgcgccag ctaacgaccc aacgcaagac accattaacc gaatatactt tgcaatgtga
    26041 atatagtcag ttaaccggca ttgaagcgtt gctggggcag tgtgacggca aaattattaa
    26101 cagtgattat caggcattcg ttctgctgcg ggtggcgctt ccggcggcga aagtggctga
    26161 attttccgca aagctggcgg attttagccg tggttcattg caattgttag cgattgaaga
    26221 ataatcccca cttcgttttg cagaactaag gaagcggcag agatgcattt tcgcgccatt
    26281 acccgaatcg ttggactact ggtcatctta ttttcaggga ccatgattat ccctgggctg
    26341 gtagcactca tctaccggga tggagcgggc cgcgctttta cccagacctt ttttgtcgcc
    26401 ctcgccattg gctctatgct ttggtggccg aaccgcaaag agaagggcga actgaaatcc
    26461 cgtgaggggt ttctgatagt ggtgctgttc tggaccgtgc tgggtagcgt cggtgcgctc
    26521 ccttttatct tctcggaaag cccgaacctc acgattaccg atgcgttttt tgaatctttc
    26581 tctggcctga ccaccacggg ggccactacg ctggtggggc tggattcgct ccctcatgcc
    26641 atcctctttt atcgccagat gctgcaatgg tttggcggta tggggatcat cgtgctggcg
    26701 gttgcgatac tgcctatcct cggcgtgggt gggatgcagc tctatcgcgc agaaatgccc
    26761 ggcccgctga aagataacaa aatgcgcccg cgaattgcgg aaacagcgaa aaccctgtgg
    26821 ttgatttatg tcttgctgac cgtcgcctgt gcgctggcgt tgtggtttgc tggaatggat
    26881 gcctttgatg ccatcggcca tagctttgcg accatcgcta ttggcggctt ctcgacacat
    26941 gatgccagta tcggttattt cgacagcccg actattaaca ctatcattgc tatcttcctg
    27001 ctgatctctg gctgtaacta cggtctgcac ttttcactgt taagtgggcg tagtctgaag
    27061 gtttattggc gcgatccgga atttcgcatg tttatcggcg tacagttttc actggtggtt
    27121 atttgtacac tcgtactgtg gtttcataat gtctacagtt cggcgctgat gacaattaac
    27181 caggcgtttt tccaggtggt gtcgatggcg acaaccgccg ggtttacaac tgacagcatt
    27241 gcccgctggc cgctcttttt gccggtactg cttttatgtt cagcttttat cggcggttgc
    27301 gccgggtcaa cgggcggtgg cctgaaagtg atccgcatcc tgctgctgtt taagcagggg
    27361 aaccgtgagc tgaaacgact ggtgcatccg aacgccgtct atagcattaa gctggggaat
    27421 cgcgcactgc cggaacgtat cctcgaagcc gtttggggat ttttctccgc ctatgcattg
    27481 gtgtttattg tcagtatgct ggcgattatc gccacgggcg tggatgactt ttctgccttt
    27541 gcctcggttg ttgcaacatt gaataacctg gggccagggc ttggcgtggt tgctgataac
    27601 tttaccagta tgaacccggt ggctaaatgg atcctgattg ccaacatgct gtttggtcgt
    27661 ctcgaggtct ttacattgct ggtgctcttt accccgactt tctggcgtga atgatggagt
    27721 aatacgtgaa aacattaatt cttttctcaa caagggacgg acaaacgcgc gagattgcct
    27781 cctacctggc ttcggaactg aaagaactgg ggatccaggc ggatgtcgcc aatgtgcacc
    27841 gcattgaaga accacaatgg gaaaactatg accgtgtggt tattggcgct tctattcgct
    27901 atggtcacta ccattctgcg ttccaggaat ttgtcaaaaa gcatgcgacg cggttgaatt
    27961 cgatgccgag tgccttttac tccgtaaatc tggtggcgcg taaaccggag aagcgtactc
    28021 cacagaccaa cagctacgcg cggaagtttc tgatgaactc gcaatggcgt cccgatcgct
    28081 gtgccgtcat tgccggggcg ctgcgttacc cacgttatcg ctggtacgac cgttttatga
    28141 tcaagctgat tatgaagatg tcaggcggtg aaacggatac gcgcaaagaa gttgtctata
    28201 ccgattggga gcaggtggcg aatttcgccc gagaaatcgc ccatttaacc gacaaaccga
    28261 cgctgaaata agcataaaga ataaaaa
//
LOCUS       DAZFFE010000059        28096 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFFE010000059 DAZFFE010000000
VERSION     DAZFFE010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.059"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            228..1729
                     /gene="caiT"
                     /locus_tag="ACHJ69_002696"
                     /pseudo
     CDS             228..1729
                     /gene="caiT"
                     /locus_tag="ACHJ69_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
     gene            1760..2902
                     /gene="caiA"
                     /locus_tag="ACHJ69_002697"
     CDS             1760..2902
                     /gene="caiA"
                     /locus_tag="ACHJ69_002697"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO5015650.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            3031..4248
                     /gene="caiB"
                     /locus_tag="ACHJ69_002698"
     CDS             3031..4248
                     /gene="caiB"
                     /locus_tag="ACHJ69_002698"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO5015651.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIAECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4321..5874
                     /gene="caiC"
                     /locus_tag="ACHJ69_002699"
     CDS             4321..5874
                     /gene="caiC"
                     /locus_tag="ACHJ69_002699"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO5015652.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYVLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREEST
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLTDMVLPADDGVSSFTQRKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHCLREVMFYLNLSEQEKDAFCERFSVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGELGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFCFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5983..6768
                     /gene="caiD"
                     /locus_tag="ACHJ69_002700"
     CDS             5983..6768
                     /gene="caiD"
                     /locus_tag="ACHJ69_002700"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO5015653.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6774..7364
                     /gene="caiE"
                     /locus_tag="ACHJ69_002701"
     CDS             6774..7364
                     /gene="caiE"
                     /locus_tag="ACHJ69_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO5015654.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7483..7878)
                     /gene="caiF"
                     /locus_tag="ACHJ69_002702"
     CDS             complement(7483..7878)
                     /gene="caiF"
                     /locus_tag="ACHJ69_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO5015655.1"
                     /translation="MCEGCVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8139..11360)
                     /gene="carB"
                     /locus_tag="ACHJ69_002703"
     CDS             complement(8139..11360)
                     /gene="carB"
                     /locus_tag="ACHJ69_002703"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO5015656.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHSMEEALAVAA
                     EVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLHAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11378..12526)
                     /gene="carA"
                     /locus_tag="ACHJ69_002704"
     CDS             complement(11378..12526)
                     /gene="carA"
                     /locus_tag="ACHJ69_002704"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO5015657.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEPYSWTQGSWTLTGGLPEAKKEDELPYHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIALIEQYRKTAK"
     gene            complement(12982..13803)
                     /gene="dapB"
                     /locus_tag="ACHJ69_002705"
     CDS             complement(12982..13803)
                     /gene="dapB"
                     /locus_tag="ACHJ69_002705"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO5015658.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(13964..14329)
                     /locus_tag="ACHJ69_002706"
     CDS             complement(13964..14329)
                     /locus_tag="ACHJ69_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO5015659.1"
                     /translation="MTYGEAYLEGWKNIFNYEGVSNRFEFWSFMIGSGVICLLPLLCW
                     WLAVTINNDYGVFIFFALPASFILTLIFAVPAIALAVRRMHDIGYSGWWVTIVVLIPV
                     TGVILLILCCLPSKSQDQA"
     gene            complement(14352..15266)
                     /gene="rihC"
                     /locus_tag="ACHJ69_002707"
     CDS             complement(14352..15266)
                     /gene="rihC"
                     /locus_tag="ACHJ69_002707"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO5015660.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNVDIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRQPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYICRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLSELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAL"
     gene            complement(15332..16282)
                     /gene="ispH"
                     /locus_tag="ACHJ69_002708"
                     /gene_synonym="lytB"
     CDS             complement(15332..16282)
                     /gene="ispH"
                     /locus_tag="ACHJ69_002708"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO5015661.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDATDIQEEWVKEAKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(16284..16733)
                     /gene="fkpB"
                     /locus_tag="ACHJ69_002709"
     CDS             complement(16284..16733)
                     /gene="fkpB"
                     /locus_tag="ACHJ69_002709"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO5015662.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(16841..17335)
                     /gene="lspA"
                     /locus_tag="ACHJ69_002710"
     CDS             complement(16841..17335)
                     /gene="lspA"
                     /locus_tag="ACHJ69_002710"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO5015663.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(17335..20151)
                     /gene="ileS"
                     /locus_tag="ACHJ69_002711"
     CDS             complement(17335..20151)
                     /gene="ileS"
                     /locus_tag="ACHJ69_002711"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO5015664.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIIKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKTKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVSDYTILGTVKGAELELLRFTHPFMDFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVIALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(20194..21135)
                     /gene="ribF"
                     /locus_tag="ACHJ69_002712"
     CDS             complement(20194..21135)
                     /gene="ribF"
                     /locus_tag="ACHJ69_002712"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO5015665.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(21143..21361)
                     /locus_tag="ACHJ69_002713"
     CDS             complement(21143..21361)
                     /locus_tag="ACHJ69_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO5015666.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSARKIAAGGQFLPMICAIAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            21464..21727
                     /gene="rpsT"
                     /locus_tag="ACHJ69_002714"
     CDS             21464..21727
                     /gene="rpsT"
                     /locus_tag="ACHJ69_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO5015667.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            complement(21823..22728)
                     /gene="nhaR"
                     /locus_tag="ACHJ69_002715"
     CDS             complement(21823..22728)
                     /gene="nhaR"
                     /locus_tag="ACHJ69_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO5015668.1"
                     /translation="MSMSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRAL
                     EERLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVA
                     DALSKRLVSSVLDAAVVEDEQIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQE
                     GLFSMKIGECGVSFWCTNPLPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNV
                     EILGEFDDAALMKAFGATHNAIFVAPSLYANDFYNDDSVVEIGRVENVMEEYHAIFAE
                     RMIQHPAVQRICNTDYSALFTPASK"
     gene            complement(22788..23954)
                     /gene="nhaA"
                     /locus_tag="ACHJ69_002716"
     CDS             complement(22788..23954)
                     /gene="nhaA"
                     /locus_tag="ACHJ69_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO5015669.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAVLAMIMANSGATSGWYHDFLET
                     PVQLRVGTLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLEGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            complement(24189..25448)
                     /locus_tag="ACHJ69_002717"
     CDS             complement(24189..25448)
                     /locus_tag="ACHJ69_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HFO5015670.1"
                     /translation="MFTNVNVDCCKTPGCKNLGLLNSQDYVAQGKNILCRECGYLFPV
                     ISEQSLNIYRNIVNHSWRGLICQCSTCGGTSLKKYGYSAQGQRRMYCHHCEKTFITLE
                     HVITTPRGAQLALMIEQGEALADIRKSLLLNSTGLSRELLKLAREANYKESRQCFPAS
                     DITLSTRDFRVKYNGSNNSLYALVTAEEQSGRVVAISTNYSPSAVEQHYQYTSNYEER
                     MSPGTLAHHVQRKELLTMRRDTLFDIDYGPAVLHQNDPGMLVKPVLPAYRHFELVRIL
                     TDEHSNNVQHYLDHECFILGGCLMANLQHIHQGRCHISFVKERGVAPATIDFPPRLFL
                     SGGVRNNVWRAFSNRNYSMAVCNLTGSKKVREMRHATLNSATRFIHFVENHPFLISLN
                     RMSPANVVSTLDILKHLWNKKLEHGTI"
     gene            complement(25577..27070)
                     /locus_tag="ACHJ69_002718"
     CDS             complement(25577..27070)
                     /locus_tag="ACHJ69_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5015671.1"
                     /translation="MQKTLMASLIGLAVCTGNAFSPALAAEAKQPNLVIIMADDLGYG
                     DLATYGHQIVKTPNIDRLAQEGVKFTDYYAPAPLSSPSRAGLLTGRMPFRTGIRSWIP
                     SGKDVALGRNELTIANLLKAQGYDTAMMGKLHLNAGGDRTDQPQAQDMGFDYSLVNTA
                     GFVTDATLDNAKERPRYGMVYPTGWLRNGQPTPRADKMSGEYVSSEVVNWLDNKKDSK
                     PFFLYVAFTEVHSPLASPKKYLDMYSQYMSAYQKQHPDLFYGDWADKPWRGVGEYYAN
                     ISYLDAQVGKVLDKIKAMGEEDNTIVIFTSDNGPVTREARKVYELNLAGETDGLRGRK
                     DNLWEGGIRVPAIIKYGKHLPQGMVSDTPVYGLDWMPTLAKMMNFKLPTDRTFDGESL
                     VPVLEQKALKREKPLIFGIDMPFQDDPTDEWAIRDGDWKMIIDRNNKPKYLYNLKSDR
                     YETLNLIGKEPDIEKQMYGKFLKYKTDIDNDSLMKARGDKPEAVTWG"
     gene            complement(27090..27851)
                     /locus_tag="ACHJ69_002719"
     CDS             complement(27090..27851)
                     /locus_tag="ACHJ69_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO5015672.1"
                     /translation="MSAKRRLLIACTLITAIYHFPAYSSLEYKGTFGSINAGYADWNS
                     GFVNTHRGEVWKVTADFGVNFKEAEFYSFYESNVLNHAVAGRNHTVSAMTHVRLFDSD
                     MTFFGKIYGQWDNSWGDDLDMFYGFGYLGWNGEWGFFKPYIGLHNQSGDYVSAKYGQT
                     NGWNGYVVGWTAVLPFTLFDEKFVLSNWNEIELDRNDAYTEQQFGRNGLNGGLTIAWK
                     FYPRWKASVTWRYFDNKLGYDGFGDQMIYMLGYDF"
BASE COUNT         7063 a         7346 c         6858 g         6829 t
ORIGIN      
        1 caacagaaca taacaaactg attaataatc aaaattaacg aaaaaatgcc ctctattcac
       61 ccgaaacaaa aatgtgataa caatcacaga atgcagctta ttgaataccc attatgagtt
      121 agccattaac gcgtccacga ggttaataat aattatatta aatgttaaca aaaataaaac
      181 aaacgggaac gcaaaataaa tgttcgtttt cacagtggaa ttaactcatg aagaatgaaa
      241 agagaaaaac gggaatagaa ccgaaggttt tctttccgcc gttaataatc gtcggcatac
      301 tttgttggct tacagtcaga gatctggatg cggcgaatgt cgttattaat gctgtattca
      361 gttacgtcac caatgtatgg ggatgggcat ttgaatggta tatggtggtg atgcttttcg
      421 gctggttctg gctggtgttt ggcccgtatg ccaaaaagcg tttaggtaac gaaccaccag
      481 aatttagtac cgccagttgg atctttatga tgttcgcctc ctgtacgtct gctgccgtac
      541 tgttctgggg atcgattgag atctactact acatctccac cccgccgttt ggcttagaac
      601 cgaactcaac aggcgcgaaa gagttggggc tggcttacag cttgttccac tggggacctc
      661 tgccgtgggc cacttacagc ttcctttcag tcgccttcgc ttacttcttc tttgtccgca
      721 aaatggaagt gattcgcccc agctccaccc tggtgccgct ggtaggtgaa aaacacgcca
      781 aagggttgtt cggcactatc gtcgacaact tctatctcgt cgccttgatc ttcgcgatgg
      841 gtaccagtct gggccttgcc acgccgctgg tgaccgagtg tatgcaatgg ttgtttggca
      901 ttccgcatac cctgcaactg gacgctatca tcattacctg ctggattatc ctcaacgcca
      961 tttgcgtcgc ttgcggtctg caaaaagggg tacgtatcgc cagtgacgtg cgtagttacc
     1021 tgagcttcct gatgctgggt tgggtgttca tcgtcagcgg tgccagcttc atcatgaact
     1081 acttcaccga ttcggtgggg atgttgctga tgtatctgcc gcgcatgttg ttctataccg
     1141 atcccacttc ccgcagggct ggaccgtgtt ctactgggca tggtgggtga tttacgctat
     1201 ccagatgagc atcttcctcg cccgcatctc ccgtggtcgt accgtgcgtg aactgtgctt
     1261 cggcatggtg ctggggctga cagcgtcaac ctggatcctg tggactgtac tcggtagtaa
     1321 cactctgctg ttgatagata aaaacatcat caacattcca aatctgatcg aacagtacgg
     1381 tgtggcgcgc gccatcatcg aaacctgggc cgctctgcca ctcagcaccg ccaccatgtg
     1441 gggcttcttc atcctctgct ttattgccac cgttacgctg gttaacgcct gctcttatac
     1501 cctggcgatg tccacttgcc gcgaagtacg cgatggtgaa gaacccccgc tgctggtgcg
     1561 tatcggctgg tcaattctgg ttggcattat cggtattgtt ctgctggcgc tcggcggcct
     1621 gaaaccgatt caaaccgcca ttatcgccgg aggatgcccg ctgttcttcg tcaacatcat
     1681 ggtgacgctc tcctttatta aagacgcgaa acagaactgg aaagattaat taacccccaa
     1741 aatatcaaga ggttgaaaga tggattttaa tttaaatgat gagcaggaac tgtttgtcgc
     1801 cggtatccgc gaactgatgg ccagcgaaaa ctgggaggcc tattttgccg agtgcgaccg
     1861 tgacagcgtc tacccggaac gttttgtcaa agccctggcg gatatgggta tcgacagtct
     1921 gctgatccct gaagagcacg gtggtctgga cgcggggttt gttactctcg ccgccgtgtg
     1981 gatggaactg ggtcgtctgg gagcgccaac ttacgtgctg tatcagttac cgggcgggtt
     2041 taacaccttc ctgcgtgaag gtacgcaaga gcagatcgac aagattatgg ctttccgcgg
     2101 caccggtaag cagatgtgga actcagcaat taccgaaccg ggcgcaggct ccgacgtggg
     2161 tagcctgaaa acgacttata cccgtagaaa tggtaagatt tatcttaatg gtagtaagtg
     2221 ttttattacc agcagcgcct acaccccgta catcgtggtg atggcgcgcg acggggcttc
     2281 tccggacaaa cctgtctaca ccgaatggtt tgttgatatg agcaaaccgg gcatcaaagt
     2341 gaccaaactt gaaaagctcg gtctgcgtat ggatagctgc tgtgaaatca cctttgacga
     2401 cgtggaactg gacgagaaag acatgttcgg tcgggaaggt aacggcttta accgcgtcaa
     2461 agaagagttc gaccatgaac gtttcctggt agccctgacc aactacggta cggcgatgtg
     2521 cgcctttgaa gatgcggcgc gctacgccaa ccagcgtgtg cagtttggcg aggctattgg
     2581 tcgtttccag ttgattcagg aaaaattcgc ccacatggcg atcaaattaa actccatgaa
     2641 aaacatgctg tatgaagcag cgtggaaagc agacaacggc accatcacct ctggcgatgc
     2701 agcgatgtgc aaatacttct gcgccaatgc ggcatttgaa gtggtggata gcgcaatgca
     2761 ggtgctgggc ggtgtcggga ttgcgggcaa ccaccgcatc agccgcttct ggcgtgacct
     2821 gcgtgtagac cgcgtttccg gaggatctga cgaaatgcag atcctgacgc tgggtcgcgc
     2881 ggtgctgaag caataccgct aagtccgtgc gctgcctgat gcgacgctga cgcgtcttat
     2941 caggcctacg ggagcacgaa tgtaggccgg ataaagcgtt tacgccgcat ccggcagtca
     3001 tgcgctacat aaatttttca ggagctaata atggatcatc tacccatgcc gaaattcggg
     3061 ccgttggccg gattgcgcgt tgtcttctcc ggtatcgaaa tcgccggacc gtttgccggg
     3121 caaatgttcg cagaatgggg tgcggaagtt atctggatcg agaacgtcgc ctgggccgac
     3181 accattcgcg ttcaaccgaa ctacccgcaa ctctcccgcc gcaatttgca cgcgctgtcg
     3241 ttaaatattt tcaaagatga aggccgcgaa gcgtttctga aattaatgga aaccaccgat
     3301 atcttcatcg aagccagtaa aggtccggcc tttgcccgtc gtggcattac cgatgaagta
     3361 ctgtggcagc acaacccgaa actggttatc gctcacctgt ccggttttgg tcagtacggc
     3421 accgaggagt acaccaatct tccggcctat aacactatcg cccaggcctt tagtggttac
     3481 ctgattcaga acggtgatgt tgaccagcca atgcctgcct tcccgtatac cgccgattac
     3541 ttttctggcc tgactgccac cacggcggcg ctggcagcac tgcataaagt gcgtgaaacc
     3601 ggcaaaggcg aaagcatcga catcgccatg tatgaagtga tgctgcgtat gggccagtac
     3661 ttcatgatgg attacttcaa cggcggcgaa atgtgcccgc gcatgaccaa aggtaaagat
     3721 ccctactacg ccggttgcgg tctgtataaa tgcgccgacg gctacatcgt gatggaactg
     3781 gtgggcatta ctcaaattgc agagtgcttt aaagatattg gcctcgcaca tctgctcggt
     3841 acgccggaaa tcccggaagg cactcagctt atccaccgta tcgaatgccc ttacggccca
     3901 ctggtagaag agaaactcga tgcctggctg gcggcacata ccatcgcaga agttaaagaa
     3961 cgctttgccg aactgaatat cgcctgtgcc aaagtactga ccgtaccgga actggaaagc
     4021 aatccacagt atgtggctcg cgaatcaatc actcagtggc aaacgatgga tggtcgcacc
     4081 tgcaaagggc cgaatatcat gccgaaattc aaaaataacc ccggacaaat ctggcgcgga
     4141 atgccctcac atggcatgga cacggctgct attttgaaaa atatcggcta cagcgaaaac
     4201 gacattcagg agttggtcag caaaggtctg gccaaagttg aggactaaac gcgtggcgtc
     4261 agtcagcttg cctgactagc gcccgctcaa taacaaacga acaaaatgga tagaggtgca
     4321 atggatatca ttggcggaca acatctacgt caaatgtggg acgatctggc ggacgtttac
     4381 ggtcataaaa cggcgctgat ttgtgaatcc agcagcggag tcgttaaccg gtatagttat
     4441 cttgagttaa atcaggagat taaccgcacg gcaaacctgt tttatgtgct ggggattcgc
     4501 aaaggcgaca aggttgctct acatctcgac aactgcccgg aatttatctt ttgctggttc
     4561 gggttggcaa aaattggcgc gattatggtg ccgattaacg cccgcctgtt gcgcgaagaa
     4621 agcacgtgga tcctgcaaaa tagtcaggcg tgcctgctgg tgaccagtgc gcaattttat
     4681 cctatgtatc aacagattca gcaggaagat gcctctcagt tgcggcacat ttgcctgaca
     4741 gatatggtac ttcccgctga tgatggcgtg agttcgttta ctcaacggaa aaatcaacaa
     4801 cctgccacct tgtgctatgc accgccgcta tcgactgacg atacggcgga aattctcttc
     4861 acctccggca ccacctcccg accgaaaggg gtggtgataa ctcattacaa cctgcgcttc
     4921 gctggatatt actccgcctg gcagtgtgca ctgcgtgacg atgacgtcta cctgacggta
     4981 atgcctgcgt ttcatatcga ttgccagtgt actgcggcga tggcggcgtt ttctgccggg
     5041 gccacctttg tgctggtcga gaaatacagc gcccgcgcct tctggggaca ggtgcagaag
     5101 taccgcgcca ccattaccga atgtattccg atgatgattc gtacgttgat ggtgcagccg
     5161 ccttcagcca acgatcggca acactgcctg cgggaagtga tgttttatct caacctgtcg
     5221 gagcaggaaa aagacgcgtt ttgtgaacgc ttcagcgttc gcttgctgac gtcttatggg
     5281 atgacggaaa ccattgtggg cattatcggc gatcgccctg gcgataaacg gcgctggccg
     5341 tcgattggtc gggcgggctt ttgctacgaa gcggagatcc gcgacgatca caatcgtccc
     5401 ctcccggctg gtgagctcgg tgaaatctgt attaaaggcg taccagggaa aaccatcttc
     5461 aaagagtatt ttctcaaccc gaaagccact gcgaaagtgc tggaagccga tggctggcta
     5521 cataccggcg ataccggata ccgcgacgaa gagggctttt tttatttcgt cgatcgccgc
     5581 tgcaatatga tcaaacgcgg tggcgagaat gtctcctgcg tggagctcga aaatatcatc
     5641 gccacccatc caaaaattca ggatatcgtg gttgtgggta ttaaagattc gattcgcgat
     5701 gaagccatca aagcatttgt ggtgctgaat gaaggtgaaa cattgagcga agaggaattc
     5761 ttctgcttct gtgaacagaa tatggcgaaa tttaaagtgc cctcttatct ggagatcaga
     5821 aaagatctgc cacgtaattg ctcggggaaa ataattagaa agaatctgaa ataaatgaaa
     5881 cggcagggaa ctaccctgtc agctaataat cacgctctaa aacaatatgc attttttgca
     5941 gggatgttgt cgtccctgaa aaagcaaaaa tggagaaaag gaatgagtga atcattacat
     6001 ctgacccgca atggatcaat tctggaaatt acccttgatc gtccaaaagc gaatgctatt
     6061 gatgcaaaaa ccagctttga aatgggcgaa gtatttctaa atttccgtga cgatccgcaa
     6121 ttacgtgtcg ccattattac cggtgccgga gagaaattct tttccgcggg ctgggattta
     6181 aaagcggcag cagaaggcga agcaccggat gctgactttg gcccgggagg ttttgcggga
     6241 ttaaccgaaa ttttcaatct cgacaaaccg gtcatcgccg ctgtgaacgg ctatgccttt
     6301 ggcggcggct ttgaactggc gctggcggca gattttattg tttgtgccga taacgccagc
     6361 ttcgccctac cggaagccaa actgggcatc gttcctgaca gcggcggcgt gctgcgtctg
     6421 ccgaagatcc tgccgcctgc catcgtcaat gaaatggtga tgaccggcag acgaatgggc
     6481 gcagaagagg cgctgcgttg gggggtagtc aaccgcgtgg ttagccaggc ggaactgatg
     6541 gataacgccc gcgaactggc gcagcagctg gttaacagcg ctccgctggc gattgcggcg
     6601 ctgaaagaga tctaccgcac caccagtgaa atgccggtag aagaagcgta tcgctatatt
     6661 cgcagcggcg tgttgaaaca ctatccatcg gttctgcatt cggaggatgc cattgaaggg
     6721 ccgctggcgt ttgccgagaa gcgcgatcca gtgtggaaag gacgttaaca accgtgagct
     6781 attacgcctt tgaggggtta attccggttg ttcacccgac ggcgtttgtc catcccagtg
     6841 cagtattgat tggcgatgtg attgtgggag ccggtgtcta catcggccca ctcgcctcac
     6901 tgcgtggtga ctacgggcgg ttgatcgtgc aagcgggagc caatattcag gatggctgca
     6961 ttatgcatgg ctactgcgac actgacacca tcgttgggga aaacggtcat atcgggcacg
     7021 gagcgatcct gcatggttgt gtgattggtc gcgatgcact ggtcggtatg aacagcgtga
     7081 ttatggatgg cgcggtcatt ggcgaagaga gcattgttgc cgctatgagc tttgtcaaag
     7141 cgggctttcg cggcgagaaa cgccagttgt tgatgggtac gccagcccgc gctgtacgca
     7201 gtgtgagtga cgacgagtta cactggaaac ggttgaatac caaagagtat caggatcttg
     7261 tcgggcgctg tcatgcatcg ttacatgaaa cgcagccact gaggcaaatg gaggaaaatc
     7321 gcccccgttt gcaggggacg acggatgtga cgccgaaacg gtgatctttc cggatgcggc
     7381 gtaaacgcct tatccgacct ggctggaaag caaccgtagg ccggataaga tgcgccagca
     7441 tcgcatccag cgaccaggtc gcacggtatc aacgaaatga cattaacgac gcatactctt
     7501 tgacaacatc atctgccact tctgctcgcg gttaagttcc gtgagcggaa cggcaggaat
     7561 acgcggcgtc tttcttacgc cgactaattt atcccggctg ttattgcgca ggcgcgcgta
     7621 aatttgctca tcgatatcaa ccacttttac cagtcgctgg cactggcatc cccgcccttc
     7681 cagcttatta gggatcatct taacttcgca gcttatttct gtgacttccg acagaatata
     7741 agtcagggta ttaaccgcct tgctgtgttc aatatcgaaa tgaatagaga tctctcttgc
     7801 tatcacccac cggttttcag ccatcatcca ttcggcgatt aacaaataga gtggtttttc
     7861 aacacatcct tcacacatac gaactccagt ttcacaaatt aaaagactgg ctatgactcg
     7921 ttttgcagca attaaacaag gccatattaa aattgtggat atatcgatag tatgcaactt
     7981 caccgtcgat aagtgtgagc taaatcctgc ttatgtcatc aaaaatatcg attccccaaa
     8041 agatttactt aatgggcaaa aaaaacggtc ctcatcagag aaccgtatta gttattcgat
     8101 caaattagcg gatgaaaaat atacgccatg acacgctatt atttgatctg cgcgtgcatt
     8161 tcctgcaccg aaattacttt ttcggtcgca tcggcattca gcgccatcgc ggtggcaaag
     8221 ccgccgttca gggtggtgtc gtaatgcact ttatattgca gcgcactgcg acggatcacg
     8281 cgggagtctt caatcgcacg acgacctgag gtggtgttga tgatgtaggt atattcgcca
     8341 ttcttaatac ggtcctgaat gtgcggacgg ccttcatgca ccttgttcac cagacgcgga
     8401 ttgatacccg cttcgcccag cacaatcgcc gtgccgtgag tcgcatccag ctcgaagccc
     8461 tgtttcagca gttttgccgc caggtccacc acgcgttctt tatcgccttc gcgcacggaa
     8521 agcagcgcac gaccgtgttt cttcatggtg gagttgctgc ccagctgcgc tttggcaaac
     8581 gcttcagcga aggtgcggcc cacgcccatt acttccccgg tagagcgcat ttctggccct
     8641 aacagcgggt caacgcccgg gaatttattg aacggcagca ccacttcttt caccgagtag
     8701 tacggcggga taacttcttt ggttacgccc tgctcagcca gcgatttgcc agccatcaca
     8761 cgcgccgcca cttttgccag cggtacgccg gtggctttgg agacgaacgg aacggtacgc
     8821 gccgcacgcg ggttaacttc aatcaggtag acttcgttgt ttttcaccgc aaactgcacg
     8881 ttcatcaggc cgcgcacctg cagttcgaag gccagtttct gcacctgctg gcgcatcaca
     8941 tcctgaattt cctgacttaa ggtgtaagct ggcagtgaac atgcggagtc accggagtgc
     9001 acacccgcct gctcaatatg ctccatgatg ccgccaatca gcaccatttc gccgtcgcag
     9061 atggcatcca cgtcaacttc tactgcgtca tcaaggaagt ggtccagcaa cactggcgca
     9121 tcgttagata cgctgaccgc tgtctggaag tagcgacgca ggtcagcttc gtcatagacg
     9181 atttccatcg cccgaccgcc gagaacgtaa gacggacgca ccaccagcgg gtagccaatc
     9241 tctttcgctt tctcaaccgc catttcgatg gtggtaacgg tggcgttcgc cggttgtttc
     9301 agtttcagac gctcaaccgc atgctggaag cgttcacggt cttccgcacg gtcgatggca
     9361 tccgggctgg taccgataac cggtacgcca gcagcttcca gcgcgcgcgc cagtttcagc
     9421 ggggtctgac cgccgtactg gacgataacg cctttcggct tctcaatacg cacaatttcc
     9481 agcacgtctt ccagagttac cggctcgaag tagaggcggt cggaggtgtc gtagtcggtg
     9541 gagacggttt ccgggttaca gttaaccata atggtttcat aaccatcttc gcgcagcgcc
     9601 agcgaggcgt gtacacagca gtagtcgaat tcgataccct gaccgatacg gttcgggccg
     9661 ccgccgagca ccatgatttt ttcacggtcg gtagacggat tcgcttcgca ctcttcttca
     9721 taagtggagt acatgtaagc ggtgtcggtg gcgaactctg ccgcacaggt atccacgcgc
     9781 ttataaaccg ggtgcaggtc atactggtcg cgcagcttgc ggatttctgc ttcacgcacg
     9841 cccgcgagtt ttgccagacg cgcatcggca aagcctttgc gtttcagctg gcgcaggaat
     9901 tcagcgtgca ggccagtgat gcccacttct gcgactttct cttccagacg caccagctct
     9961 tcaatctgta ccaggaacca gcggtcaatg ttggtcaaat taaatacacc gtctacagac
    10021 aggcccgcgc ggaaggcatc agcgatatac cagatacgct ctgcgccagc atctttcagt
    10081 tcgcggcgga ttttagtcag cgcttccggg tcgtcgagac tcactttcgg atcgaagcca
    10141 gttgcgccca cttccaggcc gcgcagcgct ttttgtaggg attcctgctg cgtgcgacca
    10201 atcgccatca cttcgccaac cgatttcatc tgagtggtca gacggtcgtt agcaccggcg
    10261 aatttctcga agttgaagcg aggaatttta gtaaccacat agtcgatgga cggctcgaag
    10321 gaggccggag tacgcccacc ggtgatgtcg ttcatcagct cgtcgagggt gtaacccacc
    10381 gccagtttcg ccgccacttt ggcaatcggg aaaccagtgg ctttcgacgc cagcgcagag
    10441 gagcgagaca cgcgtgggtt catttcgata acaatcaggc gaccgttttt cgggttcacc
    10501 gcaaactgga cgttggaacc accggtttca acgccgattt cacgcagtac cgccatcgag
    10561 gcgttacgca tgatttgata ttctttgtcg gtcagcgttt gggctggcgc gacagtgatg
    10621 gagtcaccgg tgtggatgcc catcgcatca aagttttcga tagagcagac gatgatgcag
    10681 ttgtcgtttt tatcacgcac cacttccatc tcgtactctt tccagccgat cagcgactca
    10741 tcaatcagca gctctttagt cggagaaaga tccagaccgc gggcgcaaat ttcttcaaat
    10801 tcttcacggt tataagcgat accgccaccg ctaccgccca tggtaaagga tgggcgaata
    10861 atgcacggga agccaacttc agcggcaacc gccagcgctt cttccattga atgagcgata
    10921 ccggaacgcg cagtttccag accgattttc ttcatcgcta cgtcgaaacg gcggcggtct
    10981 tcggctttat caatcgcatc ggcagtggca ccaatcatgg tgacgccgaa ctcttccagc
    11041 acgccctgac gctcaagctc cagcgcacag ttcagcgccg tctgcccccc catggttggc
    11101 agcaccgcgt ccgggcgctc tttttcaata atcttgcgta ccacttccca gtgaatcggc
    11161 tcgatgtagg ttgcatcagc catttccggg tcggtcatga tagtcgccgg gttggagttc
    11221 acgagaatga cgcggtaacc ctcttcacgc agggctttac acgcttgcgc gccagagtag
    11281 tcaaactcac acgcctgacc gataacaatc gggcccgcac ccagaatcag gatacttttt
    11341 atatctgtac gttttggcat ggctctttta ctcctgatta cttagcggtt ttacggtact
    11401 gctcaattaa cgcgataaag tggtcaaaca acggcgcggc gtcgtgtgga ccagggctgg
    11461 cttccgggtg tccctggaag ctgaacgccg gtttatcggt acgatgaatg ccctgtaacg
    11521 taccgtcgaa caaggattta tgtgtgacac gcaggtttgc aggtaatgtt gcttcatcca
    11581 ccgcaaaacc gtggttctgg gcggtgatca tcaccacgtt tttctcaaca tctttaaccg
    11641 gatggttgcc gccgtggtga ccaaatttca ttttgacagt cttcgcaccg ctcgccagcg
    11701 ccagcagctg atgaccgagg cagatgccga ataccggaat atcagtttcg aggaatttct
    11761 ggatagcggt aatggcgtaa tcgcacgggg ccgggtcgcc aggaccgttg gagaggaaga
    11821 tgccgtccgg attcattttc agcacatctt ccgcagaagt ttgcgccgga acgatggtca
    11881 ggcgacagcc tctatccacc agcatacgca ggatgttgcg cttggcaccg aaatcgtagg
    11941 ccacgacgtg gtacggcagc tcgtcttctt ttttcgcttc tggcaggccg ccggtcagtg
    12001 tccagctccc ttgtgtccag ctatagggtt ctgcggtggt tacttctttt gccagatcca
    12061 tgccgttcag acctgggaac gcgcgggctt tttctaatgc cagcgccgca tccgggttat
    12121 cgcctgcgat aatgcagcca ttctgagcac ctttctcacg cagtaaacgc gtcagcttac
    12181 gggtatcgat atcggcaatc gccacgatgt tatggcgctt caggtaagaa gagaggtctt
    12241 cggtattacg gaagttgctg gcaatcagcg gcaggtcgcg aatcaccaga ccttgggcat
    12301 gaacctgaga agattcttca tcggcgtcat tagtgccgac attgccaata tggggataag
    12361 taagagtaac gatttgacga gaataggaag gatcagtgag gatttcttga taaccggtca
    12421 ttgaagtatt gaaaacgact tccccaaccg ccgaacctgt tgcccctatg gcccgaccgt
    12481 gaaactgggt tccgtcttcc agaaccaata gcgctgactt aatcaaaaca ccctccagag
    12541 aatattcact caccttattt gcatattaat tcacaataat gacgtaaatc aatgcaaatc
    12601 tgcttaccga cgaatttctg gcaaacggcg gcattctgga gatatgggag ctaaaagtca
    12661 acttaatggc gggatttttt atattatttt gtggctcatg catgttttac aggcattaag
    12721 cgacaaaaag atcatataac atgttctgga aaacgcttgc gcagcaagaa gattgcatga
    12781 aatcaaaatg agcagaaaaa agtggaccaa atggtcaaaa tttacattaa aacaacaaaa
    12841 acagaataca aaacatgtaa ttttcagcat ttgcattaga tttaaaagca taacactaaa
    12901 aataaaaggg ccaaaaatta aagcccttta tattcaacga attaaatgtg ttgtttttgc
    12961 atcaccataa atatcttgtg gttacagatt attgagatca agcacatctc gcatatcaaa
    13021 aagaccactt tccttaccac tcaaccacaa agccgatctt accgcaccgt tagcaaatgt
    13081 catgcggctg gatgccttat gggtgatctc cagacgctcg ccaatatcgg caaacatcgc
    13141 ggtatgttcg ccaacgatgt cacctgcacg cacggtggca aaaccaatgg tgccaggcac
    13201 acgttcaccg gtatggcctt cacgactgta gaccgcgcaa tctttcagat ctttatcaag
    13261 agcgtgggcg atcgcctctc ccattgccag tgcggtgcct gacggcgcat caactttatg
    13321 tctatgatgt gcttcaataa tttcgatatc ggtgtagtcc cccatcactt tggctgcttt
    13381 ctccagcagt ttaagcatga cgttaacacc aacgctaaaa ttggcggcaa agacaatcgc
    13441 aatatcggcg gcggcgtcac gaatcgcctg tttaccggct tcgtcaaacc ccgtagtgcc
    13501 gatcaccatc cctttgccat gctggcgaca aaaagcgaga tggttcagcg taccttccgg
    13561 acgggtaaaa tcgataaaca catcaaaatc atcttttatc gcatcaagac tactttgcac
    13621 agtaacgcct gttttcccgg ctccggccag ttcgcccgca tcgctgccca gtaaagaaga
    13681 tccttcgcgc tccagcgccg cgcccagctg cacgccttct aatgccagcg ccgcctgaat
    13741 caactggcgg cccatacggc ccccggctcc cgcgatggca acgcggatgt ttgcatcatg
    13801 catagctatt ctcttttgtt aatttgcata gaccgttttc agagtaacca gcgcatcgct
    13861 acgccgccag ccaaaaacgc tgataattag aaattaatga taaacaggga gggatatcag
    13921 tgaaaggcat gacgcagaaa gtgatgcgtc ataccctctg tcattatgcc tggtcttgtg
    13981 actttgacgg caggcagcac agtataagca gaattacgcc cgtaacaggg atcaacacga
    14041 caatggtaac ccaccaccct gaatatccga tatcgtgcat ccttctgacg gctaacgcta
    14101 tcgccggtac ggcaaaaatc aacgttaaaa taaatgacgc gggcagcgca aaaaaaatga
    14161 aaacaccata gtcattgtta attgttaccg ctaaccacca acataaaagc ggaagcagac
    14221 agataacgcc actaccaatc ataaacgacc agaactcgaa gcgattggaa acaccttcat
    14281 aattaaagat gtttttccac ccttccagat acgcttctcc atacgtcata gaacacttcc
    14341 ttgttttatt attagagcgc cagggccagc acctcagcca cccattgctg gaagcctttc
    14401 acatccagat ccagcgccac ctgtacattg gctggtttgc ccaagcaacc gtcgatatca
    14461 acaaccgtcg tgcctgaggt aaattcaccc tgagtttcta ccgcgacaaa acacggtttg
    14521 agagtgaaca attccgggcg caccagccag gcgatagcgc aaagatcgtg catacgtagt
    14581 ccactttgca tactgccgct acggtagtgg ctaaacaggg cgtgtagcat ttttccggtg
    14641 cgattcaatt ccgacaaagt agcgagatag tcaggggtta atattgcctg attggtgaca
    14701 tccagaccgc acatgacgat ttcgatgccg ctacggaaga cacaggctgc cgcttctgga
    14761 tcggcagcga tattaaactc ggcgtttggt gtgcagttgc cgcgaccagc agaaccgccc
    14821 ataatcacca gacggcaaat atacggctta cattccgggc attgtgaaag taacagcgca
    14881 atattggtta acgggccgat ggctaccagg gtaacgggct ctggcgcacg catcagggca
    14941 tcacgaattg ccagaaacgc cggtataccc agtggctgac ggttgtgttc aacaaagtca
    15001 tagccagcca ttcccgattc accgtgtaca gatgccgcat cgcgcggcgc acgaaccagt
    15061 ggtacggcag cgccctgggc aagcggaata tccacattcc agaaatgcag cagttgcagg
    15121 gcgttgcggg tcgttttctc taccgagaca ttacccgcga cagtggtcat cagttgcaga
    15181 tcgagttcgg gggcaaaaat cgcggcggca atggcaacgg catcgtcaat gccggggtct
    15241 gtatcgagga agataggtaa acgcatgttt tctccataaa aaatgccggt aacaagaccg
    15301 gcattttcgc ataacttagg ctgctaatga cttaatcaac ttcacgaata tcgacacgca
    15361 gctctttcgg cacttcgaaa acaatgtttt cttcacgacc ttccagcgga atggcttcgc
    15421 caccgccaag ctgctgtaaa cgtgcgacta cgttctgtac cagaatatcc ggagctgatg
    15481 cgccagcagt cacgccgacg catttcgcct ctttcaccca ctcttcctgg atatctgtcg
    15541 catcgtcaat caaaaacgcg cgtttgccca tacgctgggc cagttccgcc agacggttgg
    15601 agttggaaga gtttttcgaa ccaaccacca gtactacttc cgcctgctct gccagggcgc
    15661 gtactgcttc ctgacggtta gtcgtggcgt agcagatgtc atctttgcgc ggaccgacaa
    15721 ttttcgggaa gcgtttacgc agcgcgtcga tcacatcaga cgtgtcatcc accgacagcg
    15781 tggtctgggt cataaaggag agcttctctt cgtttttgac cgtcagtttc cacacatcgt
    15841 ctggcgattc gaccagatac attccccctt ccgggttgct gtactggccc atcgtccctt
    15901 ccacttccgg gtgcccggcg tgaccgatga gaatagattc ttcaccacga cggctggcgc
    15961 gggcgacttc catatgcact ttggtcacca gcggacaggt ggcatcaaac accgtcaaat
    16021 cgcggctttt cgcttcgtta cgtactgcct gagaaacacc gtgtgcggag aaaatcagga
    16081 tcgcgccgtc cggtacttcg ctaatctgct caataaagat agccccacgc tcgcgcaagc
    16141 tatcgaccac gtagcggtta tgcaccactt cgtgacggac atatatcggt gcgccgtaaa
    16201 tggccagcgc gttttcaaca atgctgatag cgcggtctac cccggcacaa aaaccacgcg
    16261 ggttggccaa caggatctgc atgttacgcc tccagtgccg gatcgatttc cagcacttca
    16321 atatcaaaat gaacggtctg cccggccagc ggatggttga aatcaacggt aatggagtcg
    16381 ccgttaattt cgcggatcac gccaggcatc tcactgccat ccattgcggt aaaaagcatg
    16441 attgcgccaa tttctggctc gcctgcatcc ataaattcac ggcgggagaa gtactgaatc
    16501 aggtccggtg acggcacgcc aaacgccgca tctggctcca gcgagaaggt ggttttatcg
    16561 cccactttca gccccagcag gtgttgctcc agcccttcag aaagagaagc atcacccagg
    16621 cggaacagcg ccggtttacc gttgttgcgg gtagactcag cggtggtgcc atcgtcgagt
    16681 tttagcgtga agtgcaccag gacggcgcta ttgctctgta cagattcaga catgcaggtt
    16741 tgctcttata ttttgaggat ttttgccgga tgcgacctac gatttcgcag caatctgtag
    16801 gccggataag atgcgtcagc atcgcatccg gcagggttta ttattgtttt ttcgctttag
    16861 aaggtaaaaa accttccagc acaatcagtg ccgcaccgac acaaatggca gtatcggcaa
    16921 ggttgaaggt ggcgaagtgc cagtcgccga catagaagtc gatcatatcg acaacgaagc
    16981 cgtgccacag gcggtcgaac aggttgccca gtgcgccgcc aataatcagc gcgtaagcga
    17041 tattgtttag cttctgcgtg gccttcgagc gatacatcat cactgccagg atcacgctaa
    17101 taccaatcgc aataccggca aagaaccaac gctgccagcc gccgctatcg gcaaggaaac
    17161 taaacgctgc gccatagtta cgcgcataat gcagattaag cgacgggaac agcgggaccg
    17221 tatcccccag agcaaagttc tggaggatca ggtatttgct gcccagatcg ataatcagca
    17281 cgactaccac cagccacagc cagcgtagcc ctgttgaaca gatcgattga ctcatcaggc
    17341 aaacttacgt ttttcaccgt caccggcgac gttgctgaca cagcggccgc agatttctgc
    17401 gtgttccgcc accttgccga catcctgggt gtagtgccag cagcgagggc acttctcacc
    17461 ttcggcttta ctcaacgcga ctttcagccc tttgaggact tcgctctgct gggcgtcagc
    17521 aggtgcgtcg ttatagtctg caacggtagc gccggaggtc aacaggacaa atcgtaattc
    17581 atcgcccagc gcggtcagtt tcgccgccag ttccggttct gcatacaagg ttactgccgc
    17641 ttccagcgag ccgcccactt tcttatcggc acgcgcttgc tcaatgactt tgttcacttc
    17701 gccacgcact ttcaacagct cgtcccagaa cgcatcgttc attgcttcac tgtctgccag
    17761 accaaacagg ccttcgtacc actcaccggt gaagacgtat ttttcacgtt cgcccggcag
    17821 gtagccccac acttcatcag cggtgaagga gaggattggt gccatccagc gaaccagcgc
    17881 ttccgcgatg tgatacagcg cagtctggca gctacgacgc gccacactgt ccgctttggc
    17941 ggtgtactga cggtctttga tgatgtcgag gtagaaggaa cccatctcaa cggagcagaa
    18001 gcgcatcaga cgctgtacca cttcgtggaa atcgtatgct tcgtacgcct tgaggatgtc
    18061 ttcctgtgcc gctttcgcac aacctacggc ccagcgatcc agtaccacca tctcttccgg
    18121 tttcaccata tcttttgccg gatcaaaacc gttcaggttt gccagcagga agcgcgcggt
    18181 gttacggata cgacgatagc tgtcggcagc acgtttcagg atctcgtcag aaacggccat
    18241 ttcaccggtg tagtcagttg atgccaccca cagacgcaga atatccgcgc ccagtttgtt
    18301 catcacatcc tgcggcgaaa cggtgttgcc gatggattta gacatcttgc ggccctgacc
    18361 atccacggta aagccgtgag tcagtacctg acgatatggc gctttgccct tcatcgcggt
    18421 agagatcatc agggaagaca tgaaccagcc acggtgttgg tcagaacctt ccagatacat
    18481 gtccgctgcg tgaccggcaa attccggacg cacgtcaaca acggaagagt gggtagatcc
    18541 ggagtcaaac catacatcca gcgtatccgg tactttcaca tactggtcag cttcgtcgcc
    18601 gaggatctct ttcgcatcga gatcccacca cgcctgaatg ccgtcaactt caacgcgttt
    18661 tgccacttct tccatcagtt cgagagtacg cggatgcagt tcttctgtgt ctttgtgcac
    18721 gaacagtgac atcggcacgc cccaggtacg ctgacgagag atacaccagt caggacggtt
    18781 agcaaccatc gactcgatac gcgcctggcc ccagtcaggg atccactgca cgcctttgat
    18841 ctctttcagt gactgcgcac gcagaccttt ctgatccatg ctgacgaacc actgcggcgt
    18901 cgcgcggaag atgatcggcg ttttatgacg ccagcagcac ggatagctgt gttgcatttt
    18961 ctcaacgtgc aacagtgcgc ctttttcctg caacaacgca atgacaatat cgttcgcttt
    19021 gaagacgtta acgccatcca gagtcgggta agtacccggc agataagtgc cgtccgggcc
    19081 aaccgggtta gcggtttcca gaccatattt ttgaccgatc acatagtcgt ccggaccgtg
    19141 gcctggcgcg gtatgaacgg caccggtacc ggcatccagc gtaacgtggt cgccgagaat
    19201 tgccggaaca tcgaagtcca taaacggatg ggtaaagcgc aacagttcca gctcggcacc
    19261 tttcaccgtg ccaagaatgg tgtaatcgct aacgccgata cgctgcatta cgctttcaac
    19321 cagatctttc gcgaggatca cggcctgacc gtcgatttgc accagcgcat aatcaaaatc
    19381 aggtgcaatg gagattgcgc ggttagcagg cagcgtccac ggcgtggtgg tccagataac
    19441 cagcgaaatt gggccgttaa cattgcttac gccaaatttc gttttcagcg catcctgatc
    19501 gaccgcctgg aaagcgacgt cgatggacgg agaagttttg tcgtaatact caacttccgc
    19561 ttctgccagt gcagaacggc agtcaacgca ccagtgcacc ggcttcgcgc ctttgtgcag
    19621 gtgaccgttg ccgatgattt tgcccagcgc gcggatgatg ttggcttcag ttttgaagtc
    19681 catggtcagg tacgggtgcg accagtcgcc cagcacgccc agacggataa agtctttgcg
    19741 ctgaccgtca acctgggtcg cagcgtattc gcggcacttg gcgcggaact cagcggcggt
    19801 gaatttctcc cccggcttac cgtattcttg ctctactttc agttcgattg gcagaccatg
    19861 acagtcccag ccaggcacat acggcgagtc atatccagaa agccctttgg acttaatgat
    19921 aatgtctttc agaatcttgt taaccgagtg accaatatga atgctgccat tcgcataagg
    19981 agggccatca tgcagaatga aggttttttt gcctttttta gccgcacgga tgatgccgta
    20041 cagatcatca tcagtccaac gcgccagcat tcccggttcg cgcttggcga gatcgccacg
    20101 catcgggaac cctgtttccg gcaaattcag ggttgattta tagtcactca tcagattctc
    20161 ggttccgtat ttcggtttga ttacataaca ggcttaagcc ggttttgtta gcccaaaaaa
    20221 ttcgcgggcg gttaattcat cacgcgcaat ctgcgctttc agttcgtcca gcgatgcaaa
    20281 tcgctgctca ttgcgtattt ttttacgcag cactacttgt atatggcgac cataaaggtc
    20341 cattgcaaca tctaacaaat gcacttccag ttgctggcga ataccggcaa ccgttgggcg
    20401 tgttccgatg tttgcaacgc cgggtaacgg cttttcgcca aggcccaaca cttctaccgc
    20461 ataaacccct ttcaccgggg aaacctgacg gcgtagcggt acattcgccg tcgggaaacc
    20521 tatagtgcgc cctaattcat caccgtggac tacacgcccg gagatagcaa acgggtgccc
    20581 cagtaaactt tctgccagag ccagattgtc atccgcaagc gcctgacgca cggcggtgct
    20641 gctgatacgc acaccacctt cgcaaaaagt ttgcgtgctg gtgatatcga agccgtattc
    20701 catgcccgct ttctgtaata acaagaaatc gccttcacga ccagcgccaa agcggaaatc
    20761 gtcacctacg gcaagaaatt ttacccgcaa gtgcttcacc agaagatcac tgatgaagtt
    20821 ttgcgcggtt aacgccgcaa aacgcctgtc aaaacgcacg cacagcacgt aatcaacgcc
    20881 acactctgca agataacgca gtttttcccg cagccgggtg agccgtgccg gggctttatc
    20941 agtagcaaac agttccagtg gttgaggttc aaaaagcatc accatcaccg gtaagttgcg
    21001 cttgcgccct tcttcctgca agccctgtaa cagcgcgcga tgaccgcgat gcacgccgtc
    21061 gaaattacca atagtcagca cacacccttc ttgcggggcc tggctgagat tatgtatgcc
    21121 gcgtatcagc ttcatgtctg gctcaaaaca gtgaaaatcg tccgagtata ccttgtacag
    21181 cggtcaaggt taaccggcga ttgagtaccg aaatcagaaa gaagtggctt ttcattgcta
    21241 tggcgcaaat catgggaaga aactgaccgc ctgctgcaat ttttctcgcg gaaaagctgt
    21301 attcacaccc cgcaagctgg tagaatcctg cgccatcact acgtaacgag tgccggcaca
    21361 ttaacggcgc ttatttgcac aaatccattg acaaaagaag gctaaaaggg catattcctc
    21421 ggcctttgaa ttgtccatat agaacacatt tgggagttgg accttggcta atatcaaatc
    21481 agctaagaag cgcgccattc agtctgaaaa ggctcgtaag cacaacgcaa gccgtcgctc
    21541 tatgatgcgt actttcatca agaaagtata cgcagctatc gaagctggcg acaaagctgc
    21601 tgcacagaaa gcatttaacg aaatgcaacc aatcgtggac cgtcaggctg ctaaaggtct
    21661 gatccacaaa aacaaagctg cacgtcataa ggctaacctg actgcacaga tcaacaaact
    21721 ggcttaattg cctgttgttg tcagctttgt aaaaaaaccc gctctggcgg gtttttttat
    21781 atccatctgc aggccaaaat tcggcctgca aagtacaaat gtttattttg aagctggagt
    21841 aaacagcgca gaatagtctg tattgcagat acgctgtact gcagggtgct gaatcatcct
    21901 ttcggcaaaa atcgcgtggt actcttccat cacgttctca acacggccta tctccacaac
    21961 cgagtcatcg ttatagaaat cattagcgta aagcgaaggt gcaacgaaaa tagcgttatg
    22021 cgtcgcccca aaggctttca tcaacgcagc atcatcaaac tcacccaaaa tttcgacgtt
    22081 caagccctgg gagttaaacc agtttaatag tttacgcccc aacattgagc gacgccccgg
    22141 aataagtaaa cgacgctctt caagacaggc aggaaacggc ttttctggta gtgggttagt
    22201 gcaccagaaa ctgacaccac attcgccaat tttcatggaa aacagccctt cctgctgagt
    22261 ggaatcgatc ggacagtcag agatgatcat atccagttta tgctgactca actgctctaa
    22321 aagcatctcg tgcgtcgatt cgaaacagcg tagatggatc tgctcgtctt ccacaactgc
    22381 ggcatccaga acactgctga ccagacgttt ggaaagtgca tctgccacac caacatcaaa
    22441 caataagttg gactctttgc gatagttgac gatatccagc atttcctggc ttaaggtgaa
    22501 cattttatcg gcatagcgat agaccagttc ccccagttcg ctgggttcca gaccacgtcc
    22561 tttacgctta aatagtttcc cttgcaggcg ctcttccagc gcccggatct gcccggtaat
    22621 ggtttgtggt gttaaataaa gcgcctccgc tgcgccaacc acagaacctt ctttgtagac
    22681 atgccagaag taatacaagt ggttgtaatt gatatgagac atgctcattt ctctccctga
    22741 taacaatgaa aagggagccg tttatggctc cccagtacat cgtcctgtca aactgatgga
    22801 cgtaaacgaa cgcgtaacca gctatatcca attaccgccg aagaaattga accgacaagg
    22861 atacctaatt ttgcccagtt aatcagttct ggatctacgc taccaaatgc caggctggca
    22921 ataaagatag acatagtaaa accgataccg cacaggatac caaccgccat aatttgctgg
    22981 taagtcgttc cctctggcag atgtgccaat ttcaaacgca gcgccaacca gcagaacaga
    23041 ctaataccca gtggcttgcc aatcagcaaa ccagcgatga tccctaatgg cagaatggag
    23101 gtcaaacctt ccagcgtgac accttgcagt gaaacgccag cattagcaaa tgcaaacagc
    23161 ggcaaaatca gatacgccac ccatggatgc aaaacatgct ccagacgttt agccggagag
    23221 cgcccatgct tctctttcaa aggaataaag aagccgacaa tgacgccagc cagggttgcg
    23281 tgaacccccg atttcaacac cgctgtccac agcaccacgc caaccagaat ataaacgccc
    23341 gtgcggcgta caccacacag attcaatacc gcgagtaccg caattgctac agccgcgacg
    23401 ccaagagagg ccatcgataa gtcattagtg tagaacaatg cgatgataat gatggcccca
    23461 agatcgtcga taatagccag agccatcaaa aagatcttca gcgctaacgg aacacgactt
    23521 cccaacagcg ccaacacacc aagtgcaaag gcaatgtcag tcgccgccgg gattgcccag
    23581 ccttcgcggg taatcggatc ggcatagtta aaagccagat agagcaatgc cgggacaatc
    23641 atcccgccga ttgcggcaat aacaggaaat gccgcctggc gcagactggc cagcgaacct
    23701 tgcatcagct cgcgtttaac ttccagacca accaacagga aaaataccgc catcagagcg
    23761 tcattgatcc atagcagcat gttcttgttg atctcaagtg tcccaacccg gagctgaacc
    23821 ggcgtctcaa gaaagtcgtg ataccatcca ctggttgcac cgctgttggc cataatcatc
    23881 gctaatacag cggcaataat gagaataatg cctcccgagg catcactgct aaagaatcga
    23941 tgcagatgtt tcacttttta tttctcttca ggtgaataga tcgttttcac gattttaccc
    24001 cgcacgaatc atcgttaaaa tcagattata gtgtaggaat agctcgcttt ttacgatcta
    24061 ttaaaagaat tgcgccatca gcgagagatg agtttttgat gcccgaaagc ggcggctgat
    24121 gaccttaaac cccattaatc atagtctaat gtcaagattt ctgaagaaat tattcggaaa
    24181 agtaataatt aaattgttcc atgctctagt tttttattcc acagatgttt gaggatatct
    24241 aatgtagaaa cgacattcgc aggagacatt cggttcaatg atataaggaa aggatggttc
    24301 tccacaaagt ggataaaacg cgtcgcactg ttcaatgttg catgccgcat ctcgcggact
    24361 ttcttactgc cagtgagatt gcatacagcc attgaataat tgcggttaga aaatgcacgc
    24421 cagacattat ttcgtacccc accactaagg aataatcgcg gtggaaaatc aatggtggcg
    24481 ggtgccacac cgcgctcttt gacaaaggaa atatggcagc ggccttgatg aatatgctgc
    24541 aaattagcca tcaggcagcc gcccaatata aagcattcgt gatcaaggta atgctgaacg
    24601 ttgttggaat gctcatcggt cagtattctg accagttcaa aatgacgata tgccggaaga
    24661 accggtttta ccaacattcc cggatcgttt tgatgtaaaa ctgccgggcc gtaatcaata
    24721 tcaaacaagg tatcccgccg catagtaagt aactctttgc gctggacatg atgtgccagc
    24781 gtccctggag acatacgctc ttcatagttc gatgtgtatt gataatgttg ctctacggca
    24841 gatggggagt aattggttga gatagcaacc accctgccgc tttgttcttc tgcggtaaca
    24901 agagcataaa gagagttatt gctaccatta tacttgacgc gaaaatcgcg ggtactcagg
    24961 gtaatatcag aagctgggaa acactgtcga ctttctttat agtttgcttc acgcgctaat
    25021 tttaacagtt cacggctaag tccagtgctg ttaagaagta atgactttct gatatccgca
    25081 agtgcctccc cttgctcaat catcaatgct aactgggctc ctcgtggtgt ggtaattaca
    25141 tgttccagag tgataaatgt tttctcacaa tgatggcaat acattcttct ctggccttgt
    25201 gcagaatatc catatttttt gagggacgtg cctccgcaag ttgaacattg gcaaatcaaa
    25261 cctctccagg agtgattcac aatattacga taaatattaa gcgactgttc agatatcact
    25321 ggaaacaagt aaccacattc acggcataaa atatttttac cctgtgcgac ataatcctgg
    25381 ctattcagca accccaggtt tttacatcct ggtgttttgc aacaatcaac gtttacgttg
    25441 gtaaacatat ccactccctg cgaatagccg tatcttgata ttgttcaacg cgaaaagaac
    25501 ctgattcaat gcgtagcact gaatcaggcg ggcatagccc taaatggcat acattacatt
    25561 tattatgatt atcaggttag ccccaggtca ccgcttctgg tttatcacct ctggctttca
    25621 ttagagaatc attatcaata tcagttttat attttaaaaa cttaccatac atctgttttt
    25681 caatatctgg ttctttaccg atcagattaa gtgtttcata acgatcagat ttcagattgt
    25741 agagatattt cggtttatta ttgcgatcga taatcatctt ccagtcacca tcacggatcg
    25801 cccattcatc ggttggatca tcctggaatg gcatatcaat cccgaaaatt aatggctttt
    25861 cgcgtttcaa tgctttttgc tcaagaacag gaaccagcga ttcaccatcg aaagtacggt
    25921 ctgtaggtaa tttgaagttc atcattttcg ctaaagtagg catccagtcc agaccataaa
    25981 cgggtgtatc tgaaaccatt ccctgtggta gatgtttacc atatttaata atggctggaa
    26041 cacgaattcc gccttcccaa aggttatcct tgcgaccgcg taatccatcc gtttcccctg
    26101 ccaaattcag ctcatacact ttgcgcgctt cacgcgttac cggaccgtta tcactggtaa
    26161 aaataacgat tgtgttatct tcttcaccca tcgctttgat tttatccagc acttttccaa
    26221 cctgtgcatc cagatagctg atattggcat aatattcccc cacaccacgc cagggtttgt
    26281 ctgcccagtc gccataaaat aaatcaggat gctgcttctg atacgcgctc atatattgtg
    26341 agtacatgtc gaggtatttt ttgggcgaag ccagggggct atgcacttcg gtaaaagcaa
    26401 catagaggaa gaaaggcttg ctgtcctttt tgttatccag ccagttgacg acttccgaac
    26461 tgacatactc accgctcatt ttatcggctc gtggagtggg ttgcccgtta cgtagccagc
    26521 ctgtcgggta aaccatgcca taacgcgggc gttctttagc gttatccagc gtggcgtcgg
    26581 taacaaagcc cgccgtatta accagtgagt aatcaaagcc catatcttgt gcttgtggct
    26641 gatcggtgcg atcgccgcct gcattcagat gcagcttacc catcattgcc gtgtcgtacc
    26701 cttgcgcttt gagtagatta gcaatcgtga gttcgttacg ccctaaggca acatctttgc
    26761 ctgaaggaat ccatgagcga attccagtac gaaatggcat ccggccggtt aatagccctg
    26821 cgcgtgaagg tgaacttaaa ggagcggggg catagtagtc agtaaatttg accccttcct
    26881 gggcaagcct gtcgatatta ggtgttttaa cgatctgatg accatatgtt gctaaatcgc
    26941 cataacctaa atcatccgcc ataataatga ctaaattagg ttgtttagcc tctgcggcta
    27001 aggcaggact aaaagcattc cctgtgcaaa ctgcaaggcc gatcaaactg gccattaacg
    27061 ttttctgcat aattattccc tgataaaaat tagaaatcat aaccaagcat ataaatcatt
    27121 tgatcgccaa agccatcgta gcccagctta ttatcgaaat aacgccacgt cacactggct
    27181 ttccagcgag gatagaactt ccaggcaata gttaaaccgc catttaaccc gttccggcca
    27241 aattgctgct ccgtgtaagc atcgttcctg tccagttcta tttcattcca gttagataaa
    27301 acaaattttt cgtcaaataa cgtaaatggt aatactgctg tccagccaac aacataacca
    27361 ttccaaccat tcgtttgacc atatttagct gatacgtagt caccagattg attatgcaat
    27421 ccaatatacg gtttaaaaaa gccccactcg ccgttccagc cgaggtaacc gaatccataa
    27481 aacatgtcca gatcgtcacc ccatgagtta tcccattggc cataaatttt gccaaagaat
    27541 gtcatatcag agtcaaagag tctgacatgc gtcattgctg aaaccgtatg atttctccct
    27601 gctacagcat gattgagtac attactttca taaaacgagt aaaattctgc ttctttaaaa
    27661 tttaccccaa aatccgcagt cactttccat acttcaccac ggtgagtgtt tacaaatcca
    27721 ctattccagt ctgcataacc cgcatttatt gaaccaaagg ttcctttata ttctaatgaa
    27781 gaatatgcag gaaaatgata gatagctgtt atcagggtac acgcaataag aagtcgtctt
    27841 ttggcagaca tataatatgt tctccattca ttgtttttat gattccatag gaattaatac
    27901 accagacaat tcatttttat taagaatgac caggccagta tatttaaaga tgactatcga
    27961 agcgtgatga agatcgcaga ttacttttgc gaacgggcgg agggatatcg tgtttcagaa
    28021 aatgtgtctg ttttaaacat caccctttga tttataggca tttttcatga tgaacttagc
    28081 attggctgct gaacgt
//
LOCUS       DAZFFE010000060        27203 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFFE010000060 DAZFFE010000000
VERSION     DAZFFE010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.060"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..536)
                     /locus_tag="ACHJ69_002720"
     CDS             complement(<1..536)
                     /locus_tag="ACHJ69_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO5015673.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDAL
                     LIELPGEEYTVAAVSCIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFSMGYQRQVTG"
     gene            complement(586..1284)
                     /gene="yjfJ"
                     /locus_tag="ACHJ69_002721"
     CDS             complement(586..1284)
                     /gene="yjfJ"
                     /locus_tag="ACHJ69_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO5015674.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAATQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(1303..1704)
                     /gene="yjfI"
                     /locus_tag="ACHJ69_002722"
     CDS             complement(1303..1704)
                     /gene="yjfI"
                     /locus_tag="ACHJ69_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO5015675.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKLMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(1831..2562)
                     /gene="rlmB"
                     /locus_tag="ACHJ69_002723"
     CDS             complement(1831..2562)
                     /gene="rlmB"
                     /locus_tag="ACHJ69_002723"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO5015676.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(2653..5094)
                     /gene="rnr"
                     /locus_tag="ACHJ69_002724"
     CDS             complement(2653..5094)
                     /gene="rnr"
                     /locus_tag="ACHJ69_002724"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO5015677.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAAEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGIVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKVGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(5133..5558)
                     /gene="nsrR"
                     /locus_tag="ACHJ69_002725"
     CDS             complement(5133..5558)
                     /gene="nsrR"
                     /locus_tag="ACHJ69_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO5015678.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(5763..7061)
                     /gene="purA"
                     /locus_tag="ACHJ69_002726"
     CDS             complement(5763..7061)
                     /gene="purA"
                     /locus_tag="ACHJ69_002726"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO5015679.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(7165..7362)
                     /gene="yjeT"
                     /locus_tag="ACHJ69_002727"
     CDS             complement(7165..7362)
                     /gene="yjeT"
                     /locus_tag="ACHJ69_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO5015680.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(7444..8448)
                     /gene="hflC"
                     /locus_tag="ACHJ69_002728"
     CDS             complement(7444..8448)
                     /gene="hflC"
                     /locus_tag="ACHJ69_002728"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO5015681.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTSAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(8451..9710)
                     /gene="hflK"
                     /locus_tag="ACHJ69_002729"
     CDS             complement(8451..9710)
                     /gene="hflK"
                     /locus_tag="ACHJ69_002729"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO5015682.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPSGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(9796..11076)
                     /gene="hflX"
                     /locus_tag="ACHJ69_002730"
     CDS             complement(9796..11076)
                     /gene="hflX"
                     /locus_tag="ACHJ69_002730"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO5015683.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLDDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(11152..11460)
                     /gene="hfq"
                     /locus_tag="ACHJ69_002731"
     CDS             complement(11152..11460)
                     /gene="hfq"
                     /locus_tag="ACHJ69_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO5015684.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(11546..12496)
                     /gene="miaA"
                     /locus_tag="ACHJ69_002732"
     CDS             complement(11546..12496)
                     /gene="miaA"
                     /locus_tag="ACHJ69_002732"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO5015685.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHLLLNIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(12489..14336)
                     /gene="mutL"
                     /locus_tag="ACHJ69_002733"
     CDS             complement(12489..14336)
                     /gene="mutL"
                     /locus_tag="ACHJ69_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO5015686.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGANQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPRYSPAPASGRRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEVPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(14346..15674)
                     /gene="amiB"
                     /locus_tag="ACHJ69_002734"
     CDS             complement(14346..15674)
                     /gene="amiB"
                     /locus_tag="ACHJ69_002734"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO5015687.1"
                     /translation="MMYRIINWLVATLLLLCAPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPVVAKRVETPAVVAPRVSEPARNPFKT
                     ESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTIA
                     IARKLRTLLNNDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSAT
                     GASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQFGHS
                     QRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERLL
                     ASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(15693..16154)
                     /gene="tsaE"
                     /locus_tag="ACHJ69_002735"
     CDS             complement(15693..16154)
                     /gene="tsaE"
                     /locus_tag="ACHJ69_002735"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO5015688.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(16126..17673)
                     /gene="nnr"
                     /locus_tag="ACHJ69_002736"
     CDS             complement(16126..17673)
                     /gene="nnr"
                     /locus_tag="ACHJ69_002736"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO5015689.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLVKAIGIDVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANTH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSQWLIPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCTQRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKMSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            17672..18811
                     /gene="queG"
                     /locus_tag="ACHJ69_002737"
     CDS             17672..18811
                     /gene="queG"
                     /locus_tag="ACHJ69_002737"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO5015690.1"
                     /translation="MSEPLDLNQLAQNIKQWGLELGFQQVGITDTDLSASEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPCKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHITWAIAQQIERRNACVVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(18794..18847)
                     /locus_tag="ACHJ69_002738"
     CDS             complement(18794..18847)
                     /locus_tag="ACHJ69_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015691.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(19081..19156)
                     /locus_tag="ACHJ69_002739"
     tRNA            complement(19081..19156)
                     /locus_tag="ACHJ69_002739"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19121..19123),aa:Gly,seq:gcc)
     gene            complement(19193..19268)
                     /locus_tag="ACHJ69_002740"
     tRNA            complement(19193..19268)
                     /locus_tag="ACHJ69_002740"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19233..19235),aa:Gly,seq:gcc)
     gene            complement(19479..20024)
                     /gene="orn"
                     /locus_tag="ACHJ69_002741"
     CDS             complement(19479..20024)
                     /gene="orn"
                     /locus_tag="ACHJ69_002741"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO5015692.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSNEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            20119..21171
                     /gene="rsgA"
                     /locus_tag="ACHJ69_002742"
     CDS             20119..21171
                     /gene="rsgA"
                     /locus_tag="ACHJ69_002742"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO5015693.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            21268..22236
                     /gene="asd"
                     /locus_tag="ACHJ69_002743"
     CDS             21268..22236
                     /gene="asd"
                     /locus_tag="ACHJ69_002743"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO5015694.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWPTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDDSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            22258..25581
                     /gene="mscM"
                     /locus_tag="ACHJ69_002744"
     CDS             22258..25581
                     /gene="mscM"
                     /locus_tag="ACHJ69_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO5015695.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     NDALNQEILQISSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTEQLAESSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGSDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(25612..25926)
                     /gene="yjeO"
                     /locus_tag="ACHJ69_002745"
     CDS             complement(25612..25926)
                     /gene="yjeO"
                     /locus_tag="ACHJ69_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFO5015696.1"
                     /translation="MSARMFILCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(25923..26237)
                     /gene="yjeN"
                     /locus_tag="ACHJ69_002746"
     CDS             complement(25923..26237)
                     /gene="yjeN"
                     /locus_tag="ACHJ69_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFO5015697.1"
                     /translation="MDDASRDPVITEDEIRELQFSAGDVAEIEQTVLSFVDTRHTRKV
                     AMVVGNTINTLKERDGPRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPI
                     IS"
     gene            complement(26300..>27203)
                     /gene="yjeM"
                     /locus_tag="ACHJ69_002747"
     CDS             complement(26300..>27203)
                     /gene="yjeM"
                     /locus_tag="ACHJ69_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO5015698.1"
                     /translation="PGYQSGLAMLSFVVFAIFAYGGIEAVGGLVDKTENPEKNFAKGI
                     VFAAIVISIGYSLAIFLWGVSTNWQQVLSNGSVNLGNITYVLMKSLGVTLGNALHLSP
                     EASLSLGVWFARITGLSMFLAYTGAFFTLCYSPLKAIIQGTPKALWPEPMTRLNAMGM
                     PSIAMWMQCGLVTVFILLVSFGGGTASAFFNKLTLMANVSMTLPYLFLALAFPFFKAR
                     QDLDRPFVIFKTHMSAMIATVVVVLVVTFANVFTIIQPVVEAGDWDSTLWMIGGPVFF
                     SLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         6365 a         7566 c         6810 g         6462 t
ORIGIN      
        1 tctccggtca cctgccgttg gtagcccatg ctaaaattat gcacttccca tttggcatgg
       61 ttttgatttt ctactttttc cagcatgtag acgggttcga tatttcccgg ttcttcgctg
      121 ttaaaaaatc gctgccagcg tttttcctgc cagtttaagg tcattgcccc catcgctttg
      181 gcgttgatcg cctcgcgcca gtgactctct ttactgatac cgtagctctc ttcatagaca
      241 aagagcttga tatcatcaat gtcatcaata tcttcgccgc cagtggtatt gatttgcagg
      301 aactcatcgc ccgaagtgta gtaacggaaa atctggctac ccccgcccag atcgatgcaa
      361 ctgacggcgg ccacggtata ttcttcaccc ggtagctcga tcagcaatgc atcttccagc
      421 aaacgaaacg ctaacgtatc gagcgtaaaa ccactattga gatgcagacc caacggaccg
      481 cgagcgatag caggcttatt atccttgcca aacagacgct ggaaaaaacc agacatattt
      541 tatccttaaa atagttgcca gccttgcagt gaggctggca aaaaattact cgccctgttg
      601 gcgttgcagt cttgctaata catcctgggc gctactttta ttgctaccgc caattccggc
      661 ttccgccagc ttttcatcga gatcgcgacc gtctgcgact ttttccaact gtgtggcagc
      721 atccaggcga gcctgacgtt cggcctgacg cgtttgcagg cgttttaagg attctgccgc
      781 tgtcgaaacg ctggaagatg cgccaacagt agaggtcgtt acagcctgtt gtgcacgctg
      841 catggcttca gtggctttaa cgacttccat ttgctgctca aactgagcga tacgctgcgc
      901 tgtcgctgta actgcttttt ccacgccatc acgagagact tccagattcg acaacacttg
      961 ctcttcagcg gtaatgagat tctcaaggcg cgcgatttct tcagcaactt cgttaatcaa
     1021 cgacggattc acgttcttgc ttaacgcttc cagcgcacga gcttccagac tggctttgcg
     1081 ctcacgtaaa tctttcagct tatcgtgact taatttcact cgcgccagca gatcaacgcg
     1141 agattttccg gctttatcga gttcagcttt agcgtcacga atatgctgtt ccagcatgcg
     1201 cacgccctgg gtttcttcaa tggactcttc cgcctgggag ataaacgatt tccccagcgt
     1261 aaataaactt tttaaaattc ccataaatac ccgtccttgt aattagtgtg aatattcttc
     1321 tgtaatttca gccagatcca atgcgttatc taccagcgag gttatctcaa gcaggatatc
     1381 ttcaagagaa gatttaagcg ataacgcacc gaaaacaatg taatactctt cctgttgcac
     1441 gctggagatc cctaccgatg acaatggcat cagcttctga tttcttaata agaaggtatt
     1501 aaattcatct ggattgctga tactacttac cggacaaata aaggtttcga tgatcatttg
     1561 gcgggaagta aaaagaatat tgatttgcaa atcgccataa tcattcattt taataattaa
     1621 tgcgctttcg ctatttgtta catcaatatt ttgttcaggt acagtttgca gcgccgtcgc
     1681 cagcgccaac ggattccatg tcatatatta ttccatatag attaagttta aatattaata
     1741 aaattaatat ttgcagtacg taattatctt accagctata gacaaaaaaa aaccatccaa
     1801 atctggatgg cttttcataa ttcagaggaa ctagctgcgc tgacgaaccg cttcaaataa
     1861 gcaaattccg gttgcaaccg aaacgttcag ggaagaaaca cttcctgcca ttgggatgct
     1921 gatcaactca tcgcaatgtt cacgggtcag gcgacgcata ccttcacctt ccgcgcccat
     1981 caccagcgcc aggcgtccgg tcattttgct ctgatagagt gtatgatccg cctcgcctgc
     2041 cgtaccgacg atccagatat tctcttcctg caacatacgc atggtgcgcg ccagattagt
     2101 cacccgaatc agcggaacgc tttctgccgc accacaggcc acttttttcg ccgtggcgtt
     2161 gagctgtgcg gagcgatctt tcggcacaat caccgcatga acaccagcgg cgtccgcgct
     2221 acgcagacac gcgccgaggt tgtgcggatc ggttacgcca tcgaggatca gcaggaacgg
     2281 ttgatcgagc gaagcgatca gatccggcag atcgttttcc tgatactgcc gtcctggctt
     2341 cacacgagcg ataatgccct gatgcacggc accgtcgctt ttctcatcga gatattggcg
     2401 gtttgccaac tggataacca cgccctgaga ctcaagggcg tgaatcagcg gtaacagacg
     2461 tttatcttca cggcctttta aaataaagac ttcctgaaaa cgttcagggg cgcgctccag
     2521 cagggcctgc actgcatgga tgccgtaaat catttcgctc attaatgtac tcgttgttta
     2581 cgttttgccg ggagcgccac gcttgcccgg cctacggata attcaccctc ttctgtttga
     2641 agagggtaat gatcactctg ccactttttt cttcgccgca cgcttcgctt tggtcgctgc
     2701 ggctattttc tgcgttttcg ccgatggctt tttcgctttt ctcgcgtctt tcttcgctgc
     2761 tttcggcttc ggttttttct cgccgcgaaa ggcgctgtct ggctcaaagt tgaccttttt
     2821 gccaacctga cgccgcttgc cgcctttttt gcctgcatcg ccttttttcg ctttctcgcg
     2881 cgccgtttta ccgacattgc gcggtgcgcg ttcgctggag atcaggctaa agtcgatttt
     2941 gcgctcgtcc atattaaccg cttcaacgcg aacttccacg cgatcgccca ggcgataagt
     3001 ctggccgctg gattcaccca tcaggcgttg ccctacctga tcaaagcgat agtagtcatt
     3061 gtccagcgaa gagacatgga ccagaccatc aatgaacaag tcgtccagac ggacgaagaa
     3121 gccaaagcca gtgacgctgg aaattacgcc tttaaagacg ttacctacct ggtcgagcat
     3181 gaagtcacac ttcagccagt cagacacatc acgcgttgct tcatcggcac gacgttccgc
     3241 catcgaacag tgctgaccca gttgcagcat ctcttccatc gaataatggt agccgccggt
     3301 ttcagtggtg ttgccctgat gcccctgctc tttcgccagc agatatttaa tggcgcggtg
     3361 caacgtcagg tcaggataac gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc
     3421 cagaccgaag tgaccacggt tttccggatc gtaaatcgcc tgtttcatcg agcgcaaaag
     3481 catggtttgc agcatttctg catcaggacg atcggcaacc gattccagta actctgcata
     3541 gtcgcgcggt tccggcttgt taccgcccgg cagttccagc cccagctccg ccagcactga
     3601 acggaaagag gtaatcgctt cggtgctcgg cttgtcgtga atacggaaca gtgccggttc
     3661 tttggctttc tcaacgaaac gcgccgccga gatattcgcc agaatcatgc actcttcaat
     3721 taacttgtgg gcgtcgttac gctgagtctg ttcgatacgt tcaatacggc gttcagcgtt
     3781 gaaaatgaac ttcgcttctt cgctctcaaa tgagatccca ccgcgttctt cacgggcttt
     3841 atccagcact ttatagaggt tatgtaactc ttcgagatgc ttaaccagcg gcgcatactg
     3901 ctcgcgcaga tcctgatcgc cttgcagtat atgccagact ttggtgtagg tcagacgcgc
     3961 gtgagaactc atcaccgctt cgtagaattt gtagccagtc aggcggcctt tcgacgaaac
     4021 cgtcatctcg cacaccatac acaggcggtc tacctgcggg ttgagcgagc acaggccgtt
     4081 agagagcact tccggcagca tcgggataac ctgcgaaggg aagtacaccg acgtaccacg
     4141 attacgcgct tctctgtcca gcggcgttgg cggacgcaca tagtagctga cgtcggcaat
     4201 cgcgacccat aaacgccagc cgccgccgcg ttttttctca cagtaaactg catcgtcaaa
     4261 gtcacgggcg tcttcgccat caatggtgac cagcggtaaa tcgcgcagat cgacacggcc
     4321 cacttttgct tcttccggca cttcttcttt cagcccggca acctgttgct caacagcctg
     4381 cggccagatg tacggaattt catgggtacg cagagcaata tcaaccgcca tgccggtgcc
     4441 catattatcg cccagcactt cgacgatttt acccaccgct ttggtacggc gagtcgggcg
     4501 ttgagtcagt tcaaccacca ccacaaagcc catccgcgcg cccatgatct gatcgggcgg
     4561 gattaagata tcgaagctca gacggctatc gtcaggaacc acaaagccga cgcccgcttc
     4621 ggtgaagtag cgaccaacaa tctggctggt ttttggcacc agtacgcgga caatacgcgc
     4681 ttcacgacga cctttacggt cagcgcccag cggctgagcc agcacctgat cgccatgaat
     4741 gcaggttttc atctgctcgc tggagagata caaatcatct ttacgccctt caactcgcag
     4801 aaagccgtag ccatcacggt ggccaataac gatacctttc accaggtcga ggcgttccgg
     4861 cagcgcatag cactgacggc gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag
     4921 gcggcgacgc aggccttcaa gctgctcttc gccttcaatg tgcagttctg ccgccagctc
     4981 atcacggctg gccggttttt cacgtttggt taaatgttcg aggataaatt cccgactagg
     5041 gatgggattc gcgtattttt cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt
     5101 tcctccgttg tcatctctga tgaagatttt cgtcactcca ccagcaataa tttataaagc
     5161 ggttgattct cttcaaccaa atcggcaagc gtgtagttat ccagttccgt aagaaaactt
     5221 tgcacggcct tagaaagcgc ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca
     5281 ctgctgcaat tcaccagcga taagggctcc agctcgcgca ccacatcacc aatacgtatt
     5341 gcactcgccg gtttacccaa gcgaatgccg ccattttttc cacgaacagc agtcacgtag
     5401 ccggcacgac taagttgatt gattattttg accatatgat tacgggagac gccgtagacg
     5461 tcagtcactt cagaaatact ggtcatccgc ccttctggca atgacgccat gtagatcagc
     5521 gcacgtaatc cgtaatcagt gaaactcgtt aactgcacat caacctcaaa agggaaatcg
     5581 gggaaaaata catttatatt gatgataaac cagccacaag ctgtgtcgct aatttatttc
     5641 agttcgggga aggaaaaaag cgaggattta acactgtgtc ggatagcgta atggcttaac
     5701 cgacacagca atgtatcaca ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag
     5761 aattacgcgt cgaacgggtc gcgcagaatc atggtttcag tacgatccgg accggtagag
     5821 atgatatcga tcggcacacc ggtcagctct tcaatgcgct tgatgtagtt cagtgccgcc
     5881 tgcggcaggc cactacgatc tttcacgccg aaggtggatt cagaccagcc cggcatggtt
     5941 tcgtaaatcg gctctacacc tttccagtcg tcagctgcca gcggagtggt agtcacttca
     6001 cgaccatccg gcatacggta agccacgcag agtttaacct ctttcaggcc atccagaacg
     6061 tccagtttag tcaggcagaa gccagacagg gagttcagct gtaccgcacg acgaacggca
     6121 acggtgtcca gccagccggt acgacgacga cgacccgtag ttgcgccgaa ttcgttaccc
     6181 tgcttgcaga ggaactcgcc cgtttcatca aacagttcgg tcgggaacgg acctgcaccc
     6241 acacgagtgg agtaagcttt gaggataccc aaaacgtaat caacataacg cgggcccagg
     6301 ccggaaccgg tcgccacgcc accagcagta gtgttggaag aagttacgta cggataagtg
     6361 ccgtggtcga tatccagcag cgtaccctgc gcaccttcaa acatgacgaa atcgccacgc
     6421 tgacgcgcct ggtcgagcag atcagaaacg tcaacaacca tagaagtcag gatgtcggca
     6481 acagccatcg tatcatccag aactttctgg taatcaaccg cttcagcttt gtagtagtta
     6541 accaactgga agttgtgata ttccatcact tctttcagtt tttcagcgaa ggtttctttg
     6601 tcgaaaaggt cgccaacacg cagaccgcga cgtgccactt tatcttcata agcaggcccg
     6661 ataccacgac cggtggtgcc gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc
     6721 agcgcaacgt gataatcaag gatcagcgga catgcttcgg acagcagcag acgctcacga
     6781 acggggatgc cacggtcttc cagttctttc atctctttca tcagcgcagc cggagacagc
     6841 acaacaccgt taccgatgat gctggttaca ttctcgcgga gaatacctga tggaataaga
     6901 tggagaacgg ttttttcacc gttgattacg agagtatggc ctgcgttgtg accgccctgg
     6961 tagcgtacaa catatttagc ccgttcagtc agaagatcga cgatcttacc tttaccttcg
     7021 tcaccccatt gggtgcccag tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg
     7081 cttaaaaatg gattctacca tcgctttttc agagttacag cactttttgc actcaaaaat
     7141 gacctcgatc agtctgtttt ttgttcagcc aatcgttttc ctcaacatgt agtagaccac
     7201 aacgcccgca accacaagtc caccgccaaa acgacgtaaa atattatcgg gcaaattggt
     7261 catcgcagag atcatcttct tccatgcctt cgggtaaagc atcggcccta aaccttccag
     7321 taccaaaacc agggcaagcg ccagccagat tgtcgaattc attcattatc cttatagaaa
     7381 aagaaaacca ccgacatccc tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt
     7441 atattaacgc gttgcggaag tcggcgtctt catgtagcgg aagaaatcgc tgtccgggct
     7501 catgaccatc acgtcctgat tgccagagaa gctgttctca taagcacgca ggctacggat
     7561 gaatgcgtag aagtccggat ctttactgaa tgcatcagca aacagtttgg ctgcttctgc
     7621 atcgccttca ccacgcatga tgcggccctg acgctcagct tctgccagcg ttctggtcac
     7681 ttcatagtcg gcagtcgcgc gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg
     7741 acgcgctacc gcttcacgct cggcgcgcat acggttgtag atcgcttcag acacttcggt
     7801 cggcaggttg atctgcttaa ttcgcacatc gacaacttca atacccagcg ccgccatact
     7861 gttcggattg atgaccggaa ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc
     7921 ggcaatggcg ttatctgctg cagaggtagt aacttcatct tctgtacccg cagaaccgga
     7981 gttcagcgcg tcacgtactt cgagggtcag acgaccacga gaatcggtga cgatgtcttt
     8041 cacgtccagg cgaccaattt cagaacgcag acggtcagag aacttacgtt tcaacagcac
     8101 ttccgcttgc gaaatgtcgc caccacccgt tgccagatag taacggctga aatcgctgat
     8161 gcgccatttg atgtaagagt cgacgatcag gtctttcttc tctttggtca caaagcggtc
     8221 ggcctggttg tccatggtct gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg
     8281 tatcttgaaa tgcagacccg gctcataaac cagaggtttg ttgtcatcgt cacgcagtac
     8341 cttaccaaaa cgcagcgtaa taccgcgctc accttctttg acgacaaaga cagacatgta
     8401 aagcactacc agcacgatga tgataatcgc gataactgac ttacgcatcg ttattccccc
     8461 tgacgctggt agtcgttacg ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga
     8521 ctggtggacg acgtgttgct tgctccactg gttgtggagg aagaggctgg cggcagacgc
     8581 agcagattgc tggcaccgtt atcgctcttc gccgcagggg cgttaccacc tttcagcatc
     8641 tggtctaacg gcagaaccat caggttgcca cctttgtcgt taaccagcac tttgcgggtg
     8701 ttacccaaca ctttttccat cgtctcgata tacagacgct cgcgagtaat ttccggcgca
     8761 gctttatatt ccggcagaag tttagcaaag cgcgccactt caccctgagc ttccaagatg
     8821 gtctgggcct tgtacgcacg tgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc
     8881 ggctgaactt cgttggtata cgcttctgct tcacgaatgt attgctgttc gttttcacgc
     8941 gcggcaatcg catcgtcaaa cgccgctttt acttcctccg gcggacgagc agcctggaag
     9001 ttgacgtcca gcagagtgat gcccatgtca tacggacgaa tcgtctcttc cagttcgcgc
     9061 tgggtatcgc tacgaatcac ggtacgacct tccgtcagaa tgcggtccat ggtgtattta
     9121 ccgataaccc cgcgcagggc gctgtcggta gcctgacgca gactgtcatc cgggctggtc
     9181 acgctataca gatatttttc cggatcggtg acgcggtact gcacgttcat ctcaacgcgc
     9241 actacgttct cgtccgacgt cagcatcaca ccagaagctg ccagttcacg cacggcttcc
     9301 acgttcaccg gtttgacttc gtcgataaac gtcggtttcc agttcagacc cggctcaacc
     9361 agatggctga atttaccaaa gcgtgttacc acgccacgtt cggcttcttt aatggtatag
     9421 aaaccactgg ccgcccagat aatgacaatc gctgccgctg cgatggtaac gacacgaccg
     9481 ccaagctgcg ggcgtgggcc ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg
     9541 ccgcccagac caccgagctt tttgctcagc ttgcggaaga tatcatctaa atcaggtggc
     9601 ccttgatcgc gaccgccttt gtttccattt ccctcagagt tgccgctagg tttgctgctt
     9661 ccccacgggt cgcggtcttg tccgttatta ccgggctgat tccacgccat gtttgtgctc
     9721 catatttgtt atgcggtgat ccctgttgtc tttcgacgcc agaggatatg actccacgct
     9781 tcagacgcta cgccgttaga tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg
     9841 acgccagtca acgatcggca tacgaacttg cagacttacg ctgccgtcct cctccatcca
     9901 ctctttttct attgcctgaa gctgataaaa acgacttctc aggcgccctt cctgcggcgg
     9961 cagacgcaat gtatgctgcg ccacctcgcc ggaaagacgc tccgtcaaag cctgaaaaag
    10021 ctgtggtatc cccgctccgg tctgcgcaga aagccagaca cggatcggtt tgttctcttc
    10081 atctctgtca atacgcggtt cgaaatcgtc cagcatatcg atcttgttca tcaccagcag
    10141 ggttgggatc tcgtgagcat caatctcttc aagaaccgta ttcaccgctt cgatgttttc
    10201 ttgtacacgc acatccgccg cgtcaatgac gtgcagcagc aatgtggctt gccgcgtctc
    10261 ttgtaacgtg gctttaaatg ccgccaccag atcgtgcggc aggtggcgaa taaaccctac
    10321 ggtatctgcc aacacggttt caccgacatc cgcaacgtca atacgccgca acgtcgggtc
    10381 gagggtggca aacaactggt ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag
    10441 agtagattta ccggcgttgg tatatcccac cagcgaaaca gtaggaacgt cggctttgat
    10501 acgcgattgc cgcccctgct cacgctgctt ttcaactctt tccaggcgcg actgtatctg
    10561 cacgatgcga ttacgcaaca aacgacggtc ggtttcgagc tgggtttcac ccggaccacg
    10621 caaaccaatc ccgcctttct gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc
    10681 cagatggcgc aactgcgcca gctcaacctg caacttaccc tcatgggtac gcgcacgttg
    10741 ggcgaaaata tctaaaataa ggccggtgcg gtcgatgaca cgacactcgc acaaacgttc
    10801 caggttacgc tcttgcgccg ggctcagggc atggtcaaaa agaacgaccg aagcacccgt
    10861 cgctttgaca gcttccgcaa tttcaactgc tttaccttca cctacaaaat actttgggtg
    10921 cggcgcttta cggctaccgg taatcacctg caatgcttcg acaccggcgg aagagaccag
    10981 agattcaaac tcctggaggt cttccatatc tttgtcttgc gtaaaataga tgtgtaccag
    11041 taccgcctgc tcaccagcat cataacggtc aaacaagcgt ataaccctct aaatagatca
    11101 gcggggaacg caggatcgct ggctccccgt gtaaaaaaac agcccgaaac cttattcggt
    11161 ttcttcgctg tcctgttgcg cggaagtatt ctgcgcgctg ctaccatgat gatagttact
    11221 gctggtaccg ccaccggcgt tgttactgtg atgagaaacc gggcgagacg ggacaacagt
    11281 agaaatcgcg tgcttgtaaa ccatctggct gaccgtgttt ttcaacagga tcacgaactg
    11341 atcaaaagac tcgatttgtc cttgcagctt aataccattc accaaataaa tagaaactgg
    11401 aacacgttcc cgacgcagtg cgttcaggaa cggatcttgt aaagattgcc ccttagccat
    11461 tctctctttt ccttatatgc ttatttgtac tttgaacctt tcgattctga aaaaattgcg
    11521 cacgatacgt ctcaattgta cacattcagc ctgcgatagc accaacaacc tgtaatactt
    11581 cgtcacgcgc ctgttctggt ttttcactgt caagccagtg aaccccttcc caaccacgca
    11641 gccaggttat ctgccgcttc gccaactgtc tcgtggcgca aacacctcga taaaccattt
    11701 catcgtatga gatttcgcct tcaaggtaag accacatctg gcgataaccc acgcaacgaa
    11761 tggaaggcaa atccgtatgc aaatctcctc gggcaaaaag cgcccggact tctgcttcaa
    11821 aacctgaagc caacatctga tgaaaacgct gctcaattcg ttgatggagc agttcacggc
    11881 tcgccggggc gatggcgaac tgatgcacct gatacggtag agcgtctcct gacgtttgcg
    11941 tcagttccgt taaagtttta cccgaaatga aaaaaacttc cagtgcccgg gaaagccttt
    12001 gtggatcatt tggatgaatc cttgctgccg caaccggatc gatctcctga agttgacgat
    12061 gcaatgactc ccaaccttgc tctgccgcct gttgctcaat tctggcccgt acttccgggt
    12121 ctgccgacgg tagcggcgac aacccttcca gcaatgcctt gaaatacaac atcgtaccgc
    12181 ccactaacag tgggatccgc cccgccgcgg tgatatcggc catttccgcc agcgcatcgc
    12241 ggcgaaaatc agcagccgag taagcctgcg acggatcgcg aatattcagc aataggtgcg
    12301 gcgcggcgag taactcttca gcgttcggct tcgccgtccc gatatccatc cctttgtaaa
    12361 taagggcaga atcaacgctt atcaactcta ctggtaaaat tttacgcagc tcaatggcta
    12421 acgccgtttt accggaggcc gtcggcccca tcaaaaaaat cgccttaggc aggctcgcct
    12481 tactgatatc actcatcttt cagggctttt atcgccggat gtaaatcaac agattgtaac
    12541 agaccacccg gcggcgtttt cacaagttgc ggacataacc gttccacgtc cgccagcagg
    12601 gttatggcct gtgccattga ccactgcgca tgttcgctca tcagatttcg tgcaatccac
    12661 tgcgcaatat tgccaggttc gaatacggac tgcttcgcca ggtagcctat cagttcagga
    12721 atcaagattt gtaaattttg ttggcgtaag ggtaaaggca cggccctgat ggtcacatgc
    12781 tgcgcatcgg actggaaatc aatacccagt tccgccaggg cagactgcgc tttttctaat
    12841 gccgattttt cttcgccaga aacttttagc cgcaacggaa taagcagcgg ttgggcgcaa
    12901 actggcactt cacccggcgt caattgtgcc tgacgcagcc aacgttctgc cactggcaag
    12961 gataaaagtg aaatgttgcc atcgcgctca agcaacgcac agtcggaatg gacgatagtc
    13021 agtacccgac caaaactctg actgttcgcc gcaagtgcag gttcctgcgg ttctggcgct
    13081 tttggttttt gcatcggcgc gggcgtttgc aaaagctggc gatacacttc accttgctgt
    13141 ttctggtaac ctggctgcgc attcggccag ggggcagccg gacgacggcc tgaggcgggc
    13201 gcaggagagt agcgcggagc taccggctca cgaactgccg gttcagcaaa gtgattgcgc
    13261 cccgccgcca cgcgattttc cggaatggca cgcggtgcag gttgtggttc atcgtccagc
    13321 ggtagcggtg tttccagttg ctgttgtagc acgctcagca cgccctgata gataaagtca
    13381 tgcaccagac gcgactgatg gaaacgcact tcgtgtttgg cagggtgcac gttgacgtcc
    13441 acctgatgcg ggtcgatctc cagatacaac acaaatgccg gttgctgatt ggcccccagt
    13501 ttgtcttcgc aggcctggcg gatcgcgtga ttgatcaggc gatcgcgcat catgcgaccg
    13561 ttcacgtagc aatactgaat ttcggccagt gcgggcgtag tgtgatttgg atcggccacc
    13621 cagccgcgca gcgtgagatc gccgtgttgc cattcaatcg ccagcgcttg ttcgagaaaa
    13681 gcggtgccgc aaatcgcgcc taagcgccgt tctttttgcc cgccttccgg cactgcgcgg
    13741 tactggcgta caattttgcc gttatgcgac aggttgatag tgacgtcgaa acgcgccagc
    13801 gcaatgcggc ggatgatttc gtcgatatgg ttaaattcgg ttttctcggt gcgcaggaat
    13861 ttgcgccgcg ccggggtgtt gtagaacagg tccagcacct ccagcgtcgt tcccaccgga
    13921 tgcgccgccg gtttaaccgt cacgtccata tcgcgccctt cggcataggc ctgccaggct
    13981 tcctgctgtt ctgcggtgcg tgaagtgagc gtcaggcggg aaaccgaact gatactcgcc
    14041 agcgcctcac cgcgaaagcc cagactgata atggcttcga gatcgtccag agaggcgatt
    14101 ttactggtgg catgacgcgc cagcgccagc gccagctcgt cttttttgat accgcagccg
    14161 ttatcacgaa tgcgaataag tttcgcccca ccgcgttcga tatcaatatc gatacgcgtc
    14221 gcacctgcat cgaggctgtt ttccaccagt tctttgacta ccgacgcagg tcgctcgacc
    14281 acctcacctg cggcaatctg gttcgccagt tgtggcggta agacctgaat tggcatcaat
    14341 cgtccttagt ttggcagcgt gcgatctggc gtcgtcaccg tactggccgt ttgtgccgtt
    14401 gcaccctgcg gcgcagattg catcggatgc gcaaggaagt aattgcgcag gcctttgtaa
    14461 atggcttctg ccagctgttg ttggtaatcg tcgctcgcca gcaaacgttc ttcgctgttg
    14521 ttactgataa aaccggtttc gaccagtact gacggaatat ccggcgaacg cagaacgccg
    14581 agactcgcgt gttctggtcg gcgtttatgt atttcgccaa tgcgttgcaa ctgactgatc
    14641 atactggtcg ctacatcata ccctacccgc tgggaatgac cgaactgtaa atccagcacc
    14701 gcctggctta aataggggtc agactgactg ttcgccagca catcgcccgc tccgcccagc
    14761 agctccgact gtttctcgtg ctgctccagc cagctggcca tttcgctgtt ggcgcgacgg
    14821 ttagagagca cccatacgga agcgccagtc gcactgcggt tcggtgctgc atcggcatga
    14881 atcgacacaa ggaaattggc gttttgctta cgtgccacat cactgcgccc catcaccgag
    14941 ataaagtaat ccccgtcacg ggttaaaacg cccttaaaca tcggatcgtt attgagcaaa
    15001 gtacgcaatt tacgcgcgat ggcgatggtg acatttttct cccgcgtacc accggggccg
    15061 atagcgccag ggtcctgacc gccgtgtccg gcatcaatag cgatgataat tttatcgcca
    15121 gtgttagccg tcgcgcgggc tgccggacgc gttaccgtat tactgctgat aacacccgta
    15181 gtgcggttac tttccgtttt aaacggattg cgcgccggtt cgctgacgcg cggtgcgaca
    15241 accgcaggcg tttcaacgcg tttcgcaacc acgggcggcg gcggtggcac atctgcgtta
    15301 atcgtaaaga cgacagtgta attgctgcca ttctgccgct tcaccgcttc ggttttacca
    15361 ttttcggtaa gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt tccagagcga
    15421 atcgccttca ccagattatt gccgctaaac aacaacggca gcccctgaat tacccccgtt
    15481 tgtttgatat cgagcgccac ggtgcgtttg ctttgatggc taaacgcata atcaggatcg
    15541 ccaataaaac tcaacgttat ccgtgcctgt tgattaccgt tagaaacctg aatatcagag
    15601 agcgtcgcgg cccccaccgg cgcgcacagc agcagcaacg tcgctaccaa ccaatttatg
    15661 atgcgataca tcatcccgcc acctttcaaa ggttaaccgg ctaaacgcgc cagcaacaat
    15721 tcacccgcag aggaaaccgc actcacgcgc gcctcacggc cttgtgcctg ataatcaatg
    15781 tgtatttcga catccgggtc aggaagaaca cctgtacctt gttgtggcca ctccaccagg
    15841 cagatggcat cgttggcaaa ataatcgcgg atccccataa actccagctc ctcgggatcg
    15901 gcaaggcggt acaaatcaaa gtgataaacc attaaattgt cgagcgtata gggttcgacc
    15961 agcgtataag tggggctttt gacattaccc tgatgaccca gagcctgtaa aaagccccgg
    16021 ctaaaggtgg ttttacctgc gcctaaatcg ccatacagat agattacggt tgcgccatcg
    16081 caggctttcg ctacccgctc gcccaggtct aatgttgcct gctcatcagg gagcggaatt
    16141 actcgattca tcatggtttt tatcagtcac ttccgggtta acaatacgct gtagcgtgga
    16201 aaagagatcg gttgccagca tcccgcgcgt tccaaaacgc gccgccagta cgtcagctgc
    16261 cgcaccgtgc gcgacacagc ccgcacaggc tgcatcataa gggctcattt tttgcccaag
    16321 caatgcgcca ataataccag agagcacatc gcccatgccg ccgctcgcca tgcctgcatt
    16381 tccggcatca ataatgccta aagcgtcagg atgggcggcg accacggttc cggcaccttt
    16441 cagcaccgct acgccgccat aacgttgtac cagacgttgg gtgcaatgta agcggtcact
    16501 ttcaatttca gcgacggaac agcctaacaa ccgtgcggcc tcgccaggat gcggcgtgat
    16561 cacgcgattg tgacgcttat cgggattgat tgccagcagg ttcaatgcat cggcatccca
    16621 caacatcggt ttgcgaaaat tctcaacttt ttgcagggct tttttccccc actcttgctg
    16681 gcccagaccg ggaccaatca ccaccacatc ggcccattcc aggctttcgg taagagagtc
    16741 catagtcagt tcatgcacca tcaattccgg tcgtgcagtc agcagcggcg caatgttctc
    16801 actgcgggtc agtactcgga ctaaaccagc accagcacgc agcgccgctt cccccgtcat
    16861 acgaatagcc cccgccgtgc cgtgatcgcc accaataatc accagccgcc cgtgatcgcc
    16921 tttatgcgaa gtcgggcgac gcggtatcag ccactgagaa agttgttctg ccgaaaaccg
    16981 ctgaattttc gtctcctgac ctgccagcca gctatccagc cccagtgagt caaaatgcag
    17041 ttgtccggta acatcccgcg cttttccagt gagcaagcct ggtttcagcg caataaaagt
    17101 gatggtgtga tcggcgttga ttaccgcgcc tggcgtagcg ccggtttcag ccagaaggcc
    17161 ggaagggata tcaaccgcca caatcggcgc agggtgagta ttagcgtggt cgattaactg
    17221 gctaatggat tcgcggggcg cttgctgcaa gccggtgccg agcagcgcat caacaatcag
    17281 atctaccgat tcgggccaga caatattcga agcatggatc tcgcctcccg cgtttaacca
    17341 tgcttcgcgt gccagcgcgg cctcttccgg caacggtttg tcactttcct gggccagcag
    17401 cgtgacatct ataccgatcg ctttgaccag ccgcgcgacc acgtagccat cgccgccgtt
    17461 attaccatga ccgcacagca ccagccagtg gcgggcgtca ggataagccg aacgacacac
    17521 ctggaatgcg gcttcgccag cgcgaagcat cagctcatag agtgtgagcc ccagcgcatc
    17581 tgccgcctcg cgttctccgc ggcggatatc gtcggcgtac cagacggtgt gtggtatact
    17641 tacggggttt ttcttcattg tatggtccgt catgtcagag cccctcgatc tcaatcaatt
    17701 agcgcaaaat attaaacagt gggggctgga actgggcttt cagcaggtag gtattaccga
    17761 taccgatctc agcgcgtccg agcccaaact gcaagcatgg ctggacaaac aataccacgg
    17821 cgaaatggac tggatggcgc gtcacggtat gctgcgtgct cgtcctcacg aattattgcc
    17881 cggtacgctg cgcgtgatca gcgtgcggat gaattacctt cctgctaacg ccgcatttgc
    17941 cagcacgctg aaaaatccca aactcggcta tgttagccgt tatgcgctgg gccgtgacta
    18001 tcacaagctt ctgcgcaacc gactcaaaaa gctgggcgag atgattcagc aacattgtgt
    18061 ttcgctgaat tttagaccgt ttgtcgattc tgcgcctatt ctcgagcgcc cgttagctga
    18121 aaaagcaggt cttggctgga caggtaagca ctcacttatc ctcaatcgcg aggccggttc
    18181 gttcttcttt ttaggcgaat tgctggtcga tattccgctg cccgtggatc aaccagtcga
    18241 ggaaggatgc ggtaaatgcg tggcctgtat gacgatttgc ccgaccggtg ccatcgtcga
    18301 gccatatacc gtcgatgctc gccgctgtat ctcttatctc accatcgaac tggaaggggc
    18361 gatcccggaa gagttgcgac cgttaatggg aaaccgtatt tacggttgcg atgactgcca
    18421 gcttatctgc ccgtggaatc gctattcgca actcactaca gaagacgatt tcagcccgtg
    18481 taagccgcta cacgcaccgg aactcattga gttattcgcc tggagtgaag agaagttttt
    18541 aaaagtcacg gaaggttcgg cgattcgccg tataggtcac ctgcgttggc tgcgtaatat
    18601 cgccgtagca ttaggcaatg ccccctggga tgaaacgatt ttgacagcgc tggaaagtcg
    18661 taaaggtgag cacccacttc ttgatgagca cataacgtgg gcgattgcgc agcaaatcga
    18721 gagacgaaat gcgtgcgtgg tcgaagtgca attaccgaaa aaacagcgtc tggttcgggt
    18781 gattgaaaaa gggttaccgc gtgacgcctg attcatccac agcttgtgaa taaaaataaa
    18841 aacgcattgc aattcaagcc ggaaaaaatc gtcaagtgat cgatacgaca aaacgaaatc
    18901 ttaattttta acttatctat caataggtta aaaaaatact attcaccgtg cgaagtttct
    18961 ttgaaattaa gattacggcc tgaggctgtg gataactctg tttacaagag tttgtcagag
    19021 acaacaaaaa ccagcaataa cgcatcgcgt cgctgtggat attttattga gagaagaatt
    19081 tggagcggga aacgagactc gaactcgcga ccccgacctt ggcaaggtcg tgctctacca
    19141 actgagctat tcccgcttgg gtggtctgtg ctttacagca cttttcaaat tttggagcgg
    19201 gaaacgagac tcgaactcgc gaccccgacc ttggcaaggt cgtgctctac caactgagct
    19261 attcccgcgt actacttaat tttgctttcg taattttcgc atttctgcgt cgttacggga
    19321 ggcgcattat acgagaaatc tttccctctg caacccccct aaaaaatttt ttttgcgttt
    19381 ttatctcaag tgctgaaatt atcagcaaga cgttcaattt agcgtcaaaa tcacgccgcc
    19441 gcaagtggat tccggcgctt acgtgaccag gaaaaatttt acagcttgat aaaatgctcg
    19501 cggtagtaag ccaactccgc caccgattca cggatatcat ccatcgcctg atgcgtcccc
    19561 tgcttggtaa agccgtcaag aatttccggc ttccagcggc gcgccagctc tttcagggtg
    19621 ctaacatcga gatagcggta gtggaagtag gcttccagct ccggcatgta tttaaacagg
    19681 aagcgacggt cctgaccgat gctgttacca caaatcggcg attttcccgc aggcacccac
    19741 tgttttaaga attcgagcgt tgccagttca gcttcacgat cgcccatcgt gctcgctttc
    19801 acacgctcta ccaacccgct ggcggtatgg gtgcgcacgt tccagtcatc catcagcgcc
    19861 agctgttcat tggactgatg tactgcaatg gtcggccctt ctgccagaat attcaggttg
    19921 gcatcggtca ccagcgtggc aatctcaata atgcgatcgc gctcgggatc cagaccggtc
    19981 atctcaagat cgatccaaat caggttgttt tcattggcac tcatgctatt ttccccccat
    20041 tcacagtgac tatattgatc cacaattgcg tgtatcatag atgttttgcc catcaggggc
    20101 gaccaggagt ctgtacgatt gagtaaaaat aaactctcca aaggccagca gcgccgcgtg
    20161 aacgccaatc accagcgtcg tcttaaaacg tctaaggaga agcccgacta cgacgacaat
    20221 ctgtttggcg agcctgatga aggtatcgtc atcagccgct ttggtatgca cgctgatgtg
    20281 gaatccgccg atggcgacgt tcaccgctgc aatattcgcc gtaccatccg ttcgttggtg
    20341 accggcgacc gcgtagtctg gcgtccgggt aaaccagcgg cggaaggcgt aaatgtcaaa
    20401 gggatcgtgg aagcggtgca tgagcgcacc tcagtgttga cgcgcccgga tttttacgac
    20461 ggtgtgaaac ctattgctgc caacatcgac cagattgtca ttgtctccgc cattttgccg
    20521 gagctgtcgc tcaatattat cgaccgttac ctggtggcct gcgaaacctt gcagattgag
    20581 ccgattattg tgctcaacaa gatagacctg ctggacgacg aaggcatgga gttcgtcaac
    20641 gagcagatgg atatctaccg caatatcggt tatcgcgtat tgatggtttc cagtcatact
    20701 caggatgggc tgaaaccgct ggaagaggcg ttgaccgggc gcatcagcat ttttgccggg
    20761 cagtctggcg ttggcaaatc cagcctgctg aacgcgttgc tggggctgca aaaagagatc
    20821 ctgaccaacg atgtctctga caactcgggg cttggtcagc acaccacgac cgccgcacga
    20881 ctgtatcact tcccgcacgg cggtgatgtg attgattccc caggtgtgcg tgagtttggt
    20941 ctctggcacc tggagccgga acaaatcact cagggctttg tcgaattcca tgactattta
    21001 ggtctgtgta aatatcgcga ttgcaaacac gataccgatc cgggctgcgc tatccgggaa
    21061 gcggttgagg aagggaaaat cgcggaaacc cgtttcgaaa actatcaccg tattctggaa
    21121 agcatggcgc aggtaaaaac gcgtaaaaac ttttctgata cggatgactg acaattaagc
    21181 taaccgtcgt tagaatcgtc cccttttttt caggatccgg catgtaggcc ggatcaggaa
    21241 cgacaaaaca atggcctgga ggctaccttg ttaaattcat ttaaactttc gctacagtac
    21301 attctgccga aactatggct tactcgcctg gcgggttggg gcgcaagcaa gcgggcagga
    21361 tggccgacaa aactggttat cgatctgttc gttaaatact acaaggtcga catgaaagag
    21421 gcgcaaaagc cggacaccgc cagctaccgc acctttaacg aattctttgt ccgtccgctg
    21481 cgtgacgaag tacgcccaat cgataccgat ccgaatgtac tggttatgcc tgccgatggc
    21541 gttatcagcc agttgggtaa aatcgaagaa gataaaatcc tgcaagccaa aggccacaac
    21601 tacagcctcg aagccctgct ggcaggcaac tatctgatgg cggatctctt ccgtaacggt
    21661 acgtttgtga ccacttacct ctccccacgt gactaccacc gcgtacacat gccgtgcaac
    21721 ggtattctgc gtgaaatgat ctacgtgccg ggcgatctct tctccgttaa ccatctcact
    21781 gctcagaacg tgccgaatct gtttgcccgt aacgaacgcg tgatctgcct gttcgatacc
    21841 gaatttggcc cgatggcgca gattctggtc ggtgcgacga ttgtaggcag cattgagacg
    21901 gtctgggcgg gcaccattac gccgccgcgc gaaggcatca tcaagcgctg gacctggcct
    21961 gccggggaaa acgacgattc tgtggcattg ctgaaaggcc aggaaatggg tcgctttaaa
    22021 cttggctcta ccgttatcaa cctgtttgca ccaggtaaag tgaacctggt tgagcaactg
    22081 gaaagcctgt ctgtcacgaa aattggccag ccgctggcag tatctaccga aacctttgtt
    22141 acgccagacg ctgaaccagc cccgcttcct gctgaagaaa tcgaggcaga acacgacgcc
    22201 agcccattgg ttgacgacaa aaaagaccag gtctaatcca tcaaaggaaa cgctgacgtg
    22261 cgcctgatta tcacttttct gatggcctgg tgcctcagtt ggggggcgta cgccgcgacg
    22321 gcccccgata gcaaacaaat cacccaggaa ctggagcagg caaaagcggc gaaacctgcg
    22381 cagccggaag tcgtagaggc gctccagtct gccttaaatg cgcttgagga acgaaaaggt
    22441 tcccttgagc gcatcaagca atatcaagaa gtcattgata attatccgaa actttccgcc
    22501 actctgcgcg cgcagttaaa caacatgcgt gacgagccac gcagcgtgtc gccgggaatg
    22561 tctaacgatg cgctgaatca ggaaattctc cagatcagca gtcagttgct ggataaaagc
    22621 cgtcaggccc agcaagagca ggagcgcgcc cgcgagattg ccgattcgct gaatcaactg
    22681 ccgcaacagc aaaccgatgc ccgccgtcag ttgaatgaaa tcgagcgccg cctggggaca
    22741 cttaccggca ataccccgct caatcaggca caaaatttcg cgttgcagtc tgactccgca
    22801 cgtctgaaag cgctcgttga tgaactggag ctggcgcagc tatctgccaa taaccgccag
    22861 gaattagcgc gcttgcgctc tgagctggcg gaaaaagaga gccagcagct ggatgcatat
    22921 ttgcaagcac tacgcaacca gttgaatagt caacgccaac tggaagcgga acgggcgctg
    22981 gaaagtaccg agcaactggc ggaaagtagc gccgatttgc caaaagatat cgtcgcccag
    23041 tttaaaatta accgcgaact atcggcggcg ctgaatcaac aggcgcagcg gatggatctc
    23101 gttgcctcgc aacagcgtca ggctgccagc cagacgttac aggtccggca ggcgttgaat
    23161 acgctgcgtg aacagtcgca atggctggga tcatctaacc tgctcggcga agcgttgcgg
    23221 gcgcaggtgg cacggctacc ggaaatgcca aaaccacaac agcttgatac cgaaatggcg
    23281 cagttacgtg tgcaacggtt acgttatgag gatctgctta ataaacagcc gctgctacgg
    23341 caaatccatc aggccgacgg tcagccgctg accgctgagc aaaaccgtat tctggaagca
    23401 cagctacgca ctcagcgtga gttgctgaac tcattgttgc agggtagcga cacgctactg
    23461 ctggagctga ccaagctgaa agtctccaac gggcaactgg aggatgcact gaaagaggtg
    23521 aacgaagcga cgcaccgcta tctgttctgg acctctgacg tgcgcccgat gaccatcgcc
    23581 tggccgctgg aaatcgccca ggatctgcgt cgtctcattt cgctggacac cttcagtcag
    23641 ttgggcaaag ccagtgtgat gatgctgacc agtaaagaga cgattttgcc gctgtttggc
    23701 gcgttgattc tggtcggttg cagtatttac tcgcgccgct atttcacccg ttttcttgaa
    23761 cgttcggcgg cgaaagtcgg caaagtgact caggatcact tctggctgac gttacgcact
    23821 cttttctggt cgattctcgt cgcgtcaccg ctgcctgtgc tgtggatgac gctgggttac
    23881 ggcttgcgcg aggcgtggcc ttatccgctg gcggtcgcga ttggtgatgg cgtaacagcc
    23941 accgtgccgc tgctgtgggt agtgatgatt tgcgccacct ttgctcgccc gaacggcttg
    24001 tttatcgctc attttggctg gccgcgcgaa cgtgtttccc gtgggatgcg ttactacctg
    24061 atgagcatcg ggcttattgt gccgctgatt atggcgctga tgatgttcga taacctcgac
    24121 gaccgcgaat tttccggttc gctgggacgg ctttgcttta tcctcatttg cggtgcgctg
    24181 gcggtggtca ccctcagcct gaaaaaggcc gggatcccgc tgtatctcaa caaagaaggc
    24241 agcggcgaca acattaccaa ccatatgctg tggaacatga tgattggcgc accgctggtt
    24301 gccattctgg cgtcggcggt gggttatctg gcaacggcgc aggcattgtt agcgaggctt
    24361 gaaacctcgg ttgccatctg gttcctgcta ctggtggttt atcacgttat ccgccgctgg
    24421 atgctgatcc agcgccgcag gctggcgttt gaccgggcga agcatcgccg ggcagagatg
    24481 ttagcgcagc gtgcgcgtgg cgaagaggaa gcgcatcatc acagtagccc ggaaggagca
    24541 attgaagtcg atgaaagcga agtcgatctc gatgccatca gtgcgcaatc cttgcggctg
    24601 gtgcgctcaa ttttgatgtt gatcgccctg ctttcggtca ttgtgctgtg gtcagaaatc
    24661 cattccgctt tcggcttcct cgaaaatatt tcgctgtggg atgtcacttc cacggtacag
    24721 ggcgtagaaa gcctggagcc gattaccctc ggtgcggtac tgattgccat tctggtgttt
    24781 atcatcacca cgcagctggt gcgcaaccta cccgcgctgc tggaactggc gattctgcag
    24841 cacctggatt taacgccggg tacgggctac gccatcacca ccatcaccaa atatctgctg
    24901 atgctgattg gcgggctggt cggcttctcg atgattggta ttgagtggtc gaaattgcag
    24961 tggctggtcg ccgcgctcgg tgttggtctg ggctttggtt tacaggagat tttcgccaac
    25021 tttatctctg gcctgattat cctgttcgaa aaaccgattc gtattggcga tacggtaacg
    25081 atccgcgatc tcaccggtag cgtgacaaaa attaacaccc gcgctaccac catcagcgac
    25141 tgggaccgta aagagataat cgtgccgaac aaggcgttta ttaccgagca gtttatcaac
    25201 tggtcgctct ctgactcggt cacgcgcgtg gtattgacga ttccggcccc tgccgatgct
    25261 aacagtgaag aagtgacgga aatcctgctc accgcagcgc gtcgctgctc gctggtgatc
    25321 gacaacccgg cgccggaagt cttcctggtg gatctgcaac aggggattca aattttcgag
    25381 ctgcgtattt acgccgctga gatgggtcac cgtatgccgc tacgccatga gatccaccaa
    25441 ctaattctgg cgggcttcca cgcccacggt atcgatatgc cattcccgcc cttccagatg
    25501 cgtctggaaa gcctgaatgg taaacaaacg gggagaacat taacttctgc gggcaaaggt
    25561 cgtcaggcgg gaagtttgta aggtctttgt ggccggatga acccatccgg cttataagca
    25621 aaactgatag cgcagaaaaa actgccacag ccaatagccg aaaatagcca gcgcggtggc
    25681 gtacataaac cagccgcgtt tttttcgcca caaagcgaac cagagaaaag ggaaaaagag
    25741 cggtaacgtc attatcacac cgacatcacg ggtatcgtca tcttcgagat acatcagcac
    25801 atcgcagacg ttattaatcc cgcgtccatc aatcatccac tctttgtcca gcgcggaagt
    25861 gatggttatc cagaggaacg ccacaataaa ccagatgcag cacaagataa acatgcgcgc
    25921 actcatgaga taatcggtcg cttaatttcg ctatagcgca tacgaaacaa atcgccgtat
    25981 cccactaatt ctccgcgaaa aaccagacat ctaataagat aagcgcaata gatatccggc
    26041 aggtttcccc accgcggacc atcgcgctct ttgagggtat taatagtgtt acccaccacc
    26101 atcgctactt tgcgggtgtg gcgggtatcg acaaaagaga ggaccgtttg ctctatttcc
    26161 gccacatccc cggcagaaaa ttgcagctcc ctgatttcat cttcggttat cactggatcc
    26221 cgactagcgt cgtccataac gtcttccttt ccagacaact catcgccgga gagacgatga
    26281 gttgcaggat ttcatatgat cagtcgagag ctaactcagg tttgttcgcc atgcgactgc
    26341 aatagttctg gtaaatcgcc atcgctaaca gcgagaagaa gacagggccg ccaatcatcc
    26401 acaatgtgct gtcccagtct ccagcttcaa ccacaggttg aatgatggtg aagacgttcg
    26461 caaatgtcac caccagtacg acaaccactg ttgcaatcat tgccgacata tgcgttttga
    26521 aaatcacaaa cggtctgtcg agatcctgac gtgctttaaa aaatgggaaa gccagcgcga
    26581 ggaacaggta aggaagcgtc atagacacgt tcgccatcag cgtcagcttg ttaaagaacg
    26641 ccgatgcggt accgccacca aacgaaacca gcaggatgaa gacagtaacc aacccacact
    26701 gcatccacat ggcgatagac ggcatcccca tcgcattcag gcgcgtcatc ggttccggcc
    26761 acaatgcttt cggcgtcccc tggatgatgg ctttcagcgg tgaatagcaa agtgtaaaga
    26821 acgcaccggt ataagcgagg aacatcgaca gtccggtaat acgcgcaaac catacgccca
    26881 gcgacaatga cgcttcaggt gacaaatgca gtgcgttacc cagcgtcacc ccgaggctct
    26941 tcatcagcac ataggtaata ttgccgaggt taacggaacc attacttaat acctgctgcc
    27001 agtttgtgct gacgccccat aaaaatattg ccagcgaata accgattgaa ataacaatag
    27061 cggcaaaaac aatacctttg gcaaagttct tttctggatt ttccgtttta tcaaccagac
    27121 caccaaccgc ttcaattccg ccataggcaa aaatagcaaa taccacaaac gataacattg
    27181 ccagaccgga ctgataacct ggg
//
LOCUS       DAZFFE010000061        26688 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFFE010000061 DAZFFE010000000
VERSION     DAZFFE010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.061"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            62..793
                     /gene="artJ"
                     /locus_tag="ACHJ69_002748"
     CDS             62..793
                     /gene="artJ"
                     /locus_tag="ACHJ69_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO5015699.1"
                     /translation="MKKLVLAALLASFTFGASAAGKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(822..1949)
                     /gene="rlmC"
                     /locus_tag="ACHJ69_002749"
     CDS             complement(822..1949)
                     /gene="rlmC"
                     /locus_tag="ACHJ69_002749"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO5015700.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKAADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWIRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(1990..2478)
                     /locus_tag="ACHJ69_002750"
     CDS             complement(1990..2478)
                     /locus_tag="ACHJ69_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO5015701.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(2538..3383)
                     /gene="potI"
                     /locus_tag="ACHJ69_002751"
     CDS             complement(2538..3383)
                     /gene="potI"
                     /locus_tag="ACHJ69_002751"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO5015702.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDSSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(3380..4333)
                     /gene="potH"
                     /locus_tag="ACHJ69_002752"
     CDS             complement(3380..4333)
                     /gene="potH"
                     /locus_tag="ACHJ69_002752"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO5015703.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGIIDQPLTILHTNFAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIMPIMWFHKHQQKSVGEHG"
     gene            complement(4343..5476)
                     /gene="potG"
                     /locus_tag="ACHJ69_002753"
     CDS             complement(4343..5476)
                     /gene="potG"
                     /locus_tag="ACHJ69_002753"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO5015704.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDVDASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(5571..6683)
                     /gene="potF"
                     /locus_tag="ACHJ69_002754"
     CDS             complement(5571..6683)
                     /gene="potF"
                     /locus_tag="ACHJ69_002754"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO5015705.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVSTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLSLKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKEATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(7033..7509)
                     /locus_tag="ACHJ69_002755"
     CDS             complement(7033..7509)
                     /locus_tag="ACHJ69_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO5015706.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(7597..8499)
                     /gene="rimK"
                     /locus_tag="ACHJ69_002756"
     CDS             complement(7597..8499)
                     /gene="rimK"
                     /locus_tag="ACHJ69_002756"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO5015707.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(8560..9282)
                     /gene="nfsA"
                     /locus_tag="ACHJ69_002757"
     CDS             complement(8560..9282)
                     /gene="nfsA"
                     /locus_tag="ACHJ69_002757"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO5015708.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(9266..9553)
                     /locus_tag="ACHJ69_002758"
     CDS             complement(9266..9553)
                     /locus_tag="ACHJ69_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO5015709.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMIPAAVVVILQVAKRLAPQLMNHPPQYSRSEREKDNDANH"
     gene            9713..9970
                     /gene="grxA"
                     /locus_tag="ACHJ69_002759"
     CDS             9713..9970
                     /gene="grxA"
                     /locus_tag="ACHJ69_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO5015710.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(10000..10377)
                     /locus_tag="ACHJ69_002760"
     CDS             complement(10000..10377)
                     /locus_tag="ACHJ69_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO5015711.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSHRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            10647..12332
                     /locus_tag="ACHJ69_002761"
     CDS             10647..12332
                     /locus_tag="ACHJ69_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO5015712.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(12508..13044)
                     /gene="rcdA"
                     /locus_tag="ACHJ69_002762"
     CDS             complement(12508..13044)
                     /gene="rcdA"
                     /locus_tag="ACHJ69_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO5015713.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSRFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERIAG"
     gene            13128..14336
                     /locus_tag="ACHJ69_002763"
     CDS             13128..14336
                     /locus_tag="ACHJ69_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5015714.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            14336..15151
                     /gene="ybjI"
                     /locus_tag="ACHJ69_002764"
     CDS             14336..15151
                     /gene="ybjI"
                     /locus_tag="ACHJ69_002764"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO5015715.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFNQ"
     gene            15236..15520
                     /gene="ybjH"
                     /locus_tag="ACHJ69_002765"
     CDS             15236..15520
                     /gene="ybjH"
                     /locus_tag="ACHJ69_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO5015716.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(15561..16793)
                     /gene="mdfA"
                     /locus_tag="ACHJ69_002766"
     CDS             complement(15561..16793)
                     /gene="mdfA"
                     /locus_tag="ACHJ69_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO5015717.1"
                     /translation="MQNKLASGVRLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            17078..17674
                     /gene="ybjG"
                     /locus_tag="ACHJ69_002767"
     CDS             17078..17674
                     /gene="ybjG"
                     /locus_tag="ACHJ69_002767"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO5015718.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            17732..18490
                     /gene="deoR"
                     /locus_tag="ACHJ69_002768"
     CDS             17732..18490
                     /gene="deoR"
                     /locus_tag="ACHJ69_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO5015719.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(18537..19739)
                     /gene="dacC"
                     /locus_tag="ACHJ69_002769"
     CDS             complement(18537..19739)
                     /gene="dacC"
                     /locus_tag="ACHJ69_002769"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO5015720.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            19986..20612
                     /gene="gstB"
                     /locus_tag="ACHJ69_002770"
     CDS             19986..20612
                     /gene="gstB"
                     /locus_tag="ACHJ69_002770"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO5015721.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(20609..21724)
                     /gene="yliI"
                     /locus_tag="ACHJ69_002771"
     CDS             complement(20609..21724)
                     /gene="yliI"
                     /locus_tag="ACHJ69_002771"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO5015722.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRMVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(21835..22218)
                     /gene="bssR"
                     /locus_tag="ACHJ69_002772"
     CDS             complement(21835..22218)
                     /gene="bssR"
                     /locus_tag="ACHJ69_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO5015723.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            22431..23756
                     /gene="rimO"
                     /locus_tag="ACHJ69_002773"
     CDS             22431..23756
                     /gene="rimO"
                     /locus_tag="ACHJ69_002773"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO5015724.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWRKICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(23803..25131)
                     /locus_tag="ACHJ69_002774"
     CDS             complement(23803..25131)
                     /locus_tag="ACHJ69_002774"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO5015725.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHIKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKSVTGDEYNAKNAIFTVSEAMEHFSYLPTGLYVF
                     AYKKDVYFQVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(25139..>26688)
                     /locus_tag="ACHJ69_002775"
     CDS             complement(25139..>26688)
                     /locus_tag="ACHJ69_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFO5015726.1"
                     /translation="FEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLVVYLMTTRVFRR
                     QFSEMTDLVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIERANKLLS
                     LISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRI
                     AEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIATVNDFDSSFGYRNLRIHKSAICEP
                     LQGENAWSYAEKLKLAISSIRNHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGEL
                     FLVYQPIVDINTRTILGAEALCRWVSAERGIISPLKFITIAEDIGFINELGYQIIKTA
                     MGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEITET
                     VIERINEHFYLNIEQLRKQGVRVSIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFG
                     TAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDK
                     SGKLVKE"
BASE COUNT         6827 a         6925 c         6369 g         6567 t
ORIGIN      
        1 ttgttatccc atgccgcaga cacggccaaa aatcataaga ttgacagacg ggagttccat
       61 catgaaaaag ttagttcttg ccgctttact tgcttctttt actttcggtg cttctgccgc
      121 agggaaaatc aattttggcg tttcagccac ctatccaccc tttgaatcta taggtgctaa
      181 taatgagatt gtcggctttg atatcgacct ggcaaaagcc ttgtgcaaac aaatgcaggc
      241 agaatgtact tttactaatc acgcgttcga cagcctgatc ccgtcactga aattcagaaa
      301 atatgacgcc gtaatctccg gaatggatat cacgccggag cgtagcaaac aggtatcgtt
      361 taccacgccc tactacgaaa actcagccgt cgtgattgcc aaaaaagata cctacaaaac
      421 gtttgccgat ctgaaaggca aacgtattgg gatggaaaac ggcaccacgc accagaaata
      481 tattcaggat cagcacccgg aagtgaaaac tgtctcctat gacagttatc agaatgcctt
      541 tatcgatctg aaaaatggcc gtattgatgg cgtatttggt gacaccgcag tggtaaacga
      601 atggctgaaa accaatccgc aactgggcgt tgctactgaa aaagtgaccg atccgcaata
      661 cttcggcacc ggcctgggta tcgctgtacg tccggataac aaagctctgc tggaaaaact
      721 gaataacgcg ctggcagcaa ttaaagctga cggtacatat cagaaaatca atgaccagtg
      781 gttcccacag taagattcag aaagccgcac ctgcggcttt tttattgctt caccagtagc
      841 gtcagcactt catagtgcgc ggtgtgcggg aacatatcaa aaagctgtac ccgttcgatg
      901 cgataaccag gcagttcgcg gatatctttc gccatagttt gggcgttaca gctggagtag
      961 ataataaaac gcggtgccat cgttgagaga taatcacaca gcggtttacc gatgccgcgg
     1021 cgcggcgggt taaccagcac cagctccggc acttccccct gagcggtggc aaactgcgta
     1081 gagtccagcg cctgaaattg caaacgcgtt aagcccagtt cagcggctga ctgcttcgca
     1141 caggcaatgg cctctggtgc aatttcgatc ccggttaact gcatgtcagg cgtcgcgcag
     1201 tgtaaaccaa agccacccac accacagaac agatcccaca tatgtttaac cggcagctgt
     1261 cgtatccagt cgcgcgcggt ggcgtacagc tggctggcga ccgccggatt agtctggaag
     1321 aaactttgcg gacggatcca cagcggtacg tcattaaaac gctctgccag tgcctgttgt
     1381 tcggtcaggt agatctccgt ctccccttcc ataatcgcca tatgtaccgg ctgaatattg
     1441 acggtaataa ctttcagctg cggtaattgt tcctgtaacc acggcagcgc cttacgcagt
     1501 tgcgccagtt tggtatcaga acgcagtaca aaacgcagca tcatgccgcc atcgctctgg
     1561 ctttcagtca gcagaatgta tttcagttca ccacgtttac gcgccacgtt gtagggcgtt
     1621 aaccccgcac gggcgataaa cggttttagc gccgcaaaaa cgggcgcaaa tgaggctgga
     1681 taaagcgggc agtcacaaag gtcttccggt gtaccatccc gatgcagcat accgagcagt
     1741 ggtttttcaa cgctaccact caccaccatt ttggctttat tacgaaaccc ttgttccggg
     1801 cctgacaccg gcgcacacca ttcctcaacc ggaaaatcgg cgagcagatt tttaagatcg
     1861 gcggctttag cggagagttg ctctggaatc ggctgcgtta tccactgaca ggaacgacag
     1921 cgacccgcgt cgtaaagtgc gcactgcata cattgacctt cacatcatca ggggcgacga
     1981 ttatacacat tattgcaact ggaagaaccg ccgactggtc gagggaacga acagcagcat
     2041 caggatgagc atatccggca gcttctgcag catcaggcta tggaagattt cacgttttga
     2101 ttcaccggga atgctaaaca gctccggata accatacccc agcgaggccg cccacaagta
     2161 acttgcggct gtgatttgtg tcaacagata cagccagcgc gcccagcgac gccctttcac
     2221 cagtgaaaac gcacaccaga tctcaatgaa aaccagcacc agactgctca aaaagaccag
     2281 cgttaaactc caggtttgta cgctgcgatg aatgaactcg ccaataccgc gcacgcccag
     2341 cgtattgaaa atcatcagca cgtcgaggcc acggatcata ataatggcga gcgccgccac
     2401 ctgcaccagc gcaggcacat tcaggcgagc atgagatgaa gatgttttct taaaaaatcc
     2461 caacgtttcg tcttccatga aaacaatgcc gcgacatgcg cggcattatg tagccaggtt
     2521 ggcaaatttt agtgtcttca gccacgtctt gcacgctgga tgtcgcgtat ccgctgtttt
     2581 tctgcgcgcg ccatcagata ccaggcgata aatccgacaa ttccgaccgc gccgaggatc
     2641 aatgttgcca gggcgttgat ttccggattc acccccatcc gcacgctgga aaataccagc
     2701 atcggtaacg tggtggctcc cggcccggaa acaaagctgg cgataaccag atcatcaagc
     2761 gacaaagtaa aagccagtaa ccagccagaa atgatcgcgg gcatgatcat cggtagcgta
     2821 atgacgaaaa acactttcag cggcgtcgca ccaagatcca tcgctgcttc ttctatcgag
     2881 ctatccagtt cccgcagacg cgacgaaata acgactgcca cataagccgt acagaaagtg
     2941 acatgcgcca gccagatggt gagcataccg cggtccgcag gccagccaat agcatgagca
     3001 agcgcgacga ataacaacaa cagcgacaaa cccgtgatga catctggcat taccagcggt
     3061 gcggtgatca tgaaggcaaa accatttgat ccgcgaaacc tgccaaagcg caccagcacg
     3121 accgccgcaa tagtcccgag gatcgccgcc gccgtcgccg cacaggccgc aattgtcaga
     3181 cttaaaccaa ccgcactcat cattgcgtca tcgcgcaata attcgccata ccagcgcgtt
     3241 gaccagccgg cccacaccgt cactaacttc gagctgttaa acgaatagat aacaagcatc
     3301 agcattggcg cgtagagaaa ggtgaagccc aacagcaaaa tcacaatccg ccagggcgaa
     3361 cgaaccaccg gtaaattatt catccgtgtt ctcccacgct tttttgctga tgtttgtgga
     3421 accacattat cggcataatt agcagcagca acatgatgat cgctaccgct gaggccaccg
     3481 gccagtcgcg gttgttaaag aactcctgcc acagcacgcg cccgatcatg atgctgtccg
     3541 ggccaccaag cagttccggg atcacaaact cgcccacagc cgggataaac accagcatcg
     3601 atccggcaat aatcccacct ttagtcagcg gcacgatcac agtaaagaac gttttcagcg
     3661 gtcgtgcacc gagatccagt gccgcttcta ccagcgaata atcaatacga atcaacgcgg
     3721 tgtaaatcgg cagcaccata aacggcacgt aggcgtaaac aatgccaata taaaccgcaa
     3781 agttggtatg cagaatggtc agcggttgat cgataatccc cagccacagc agaaaattat
     3841 tcagcacacc gttgtttttt aatattccca tccaggcgta gacacgaatc agaaacgagg
     3901 tccacgacgg caggatcacc agtagtaata agatattacg ggtcgaaggc ttactgtgcg
     3961 ccaccgccca cgccagcgga tagccgatca gtaaacagca aatagtcgaa atcgccgcca
     4021 cctgtaacga ctggagataa gcatcaaaat agagcggatc gtcggtcagt tgcagaaaat
     4081 taccgagatt aagagtgata gaaagttgcc cgtcagccca ctccatcaat tcggtataag
     4141 gtggaatagc gcgcgccatc tccgccaggc ttattttaaa gacgatcaga aatggcagca
     4201 gaaacagcaa gatcaaccag atatatggca acgcaatgac cagtttgcgc ccatgcttca
     4261 tttgcagctg cgacaaccac agcttaaatc cgcccggcgg tttcgactgg gcagcaggtt
     4321 caagtgtact catctttgct ccttaaaccg tcagcaccac acagctgtcc acttcccagc
     4381 acaaacgtac ttcgtcgccc caggtcggta accctttacg atggcgatgg gcgttttgta
     4441 gctgggcgct aatcatctgc ccacttttca ggcgaacgtg atacaccgaa agatcgccga
     4501 gataggcaat gtgtatcacc tcccccaccg cgaagttaca accattggcg ggcggttcct
     4561 cgcaaagcat gattttttcc gggcgaagcg ccacatgcac cggcacgtta tcgaccactg
     4621 aagcatccac atcgactttc agtggatgca ccagccccgg cgaataaagc accagaccat
     4681 cttcctgacg ctctttgagt acaccttcaa agacattaac cgaaccgata aattcagcgc
     4741 tgtagcgagt ggtcggatgc tcgtagatct cttccggctc gccaatctgg acaaatttcc
     4801 cacgattcat aatggcgatg cgccccgcca tagtcatcgc ctcttcctga tcgtgagtaa
     4861 ccattacgca ggtcacaccg acgcgctcca gaatatccac cacttcaagc tgcatccggt
     4921 cacgcagctt tttatccagc gcgcccatcg gctcatcgag cagtaatagt ttcgggcgct
     4981 tggcaaggct tctggccagg gccacacgct gacgctgacc accggaaagc tgatgcggtt
     5041 tgcgtttggc gaactcctgc atatgcacca gcccaagcat ctcattgacc cggctggcaa
     5101 tttccgcttt cggtagtttg tcctgcttca ggccaaaagc gatgttctgt tccacggtca
     5161 tatgcggaaa cagcgcgtaa gactgaaaca tcatattgat ggggcgcagg taaggcggca
     5221 cctgtgacaa atcgacgcca tcgagcataa tctgcccggc agaaggttgt tcaaaacctg
     5281 ccagcatacg cagcagcgtg gacttgccac agccggatgc gcccagcagc gcgaagattt
     5341 cacctttgta gatggtcagg ctgacatcat ccaccgcatg ttgaccatcg taggatttgg
     5401 tcaggttacg gatttctaat agcggcgtca gcgccttacg ggttttcgcc tgcgggcgag
     5461 ggatagcgtc attcacggct gctctccggc attgaatgtg tgcaagcgca ccatgatggt
     5521 gcgaattgcc ggcgggtgtg gtgcgcccgt ccggcatcta cggctgcgga ttattttccg
     5581 ctcttcactt tggtccacgc gcgggtgcgc acacggtcga ttttcgggtc ctgcactttc
     5641 agagtgaaca gcttcgcacg aacatccgca ggcggataaa tacccgggtt atcacggact
     5701 tccgcactca ccagcggcgt ggcttcttta ttagcattag catagaacac atggtcagag
     5761 atatgtgcta ccacatcagg acgcagcagg taattcagga actgataggc ttcgtctttg
     5821 tttttggcat ccgcaggcat ggcgaataca tcaaagaacg ccatcgcccc ttcttttgga
     5881 atcgagaacg agacattcac gccattcttc gcttccttcg cgcggtttga cgcctgccag
     5941 acatcgcccg cccagccaat agcgacacag atgtcaccat tcgccaggtc attgatgtat
     6001 tgggatgaat ggaaataacg aatgttcggg cgcagcttta acagcagatc ggtagccggt
     6061 ccggtgtaat catccgcttt agtgctgtta ggatctttgc cgagataatt cagcacggta
     6121 gcaaacactt cttctggcgc atccaggaaa gagacaccgc agcttttcag tttttccaga
     6181 ttttcaggtt tgaggctcaa gtcccagcta tcgacgggcg cgttttcgcc cagcaccgct
     6241 ttaactttat cgacgttata gccaatcccg gtcgtcgccc acatataagg catagcaaat
     6301 ttattgtcgg gatcgtgttt ggcgaccaac ttcagcagct ccggatcgag attcttccac
     6361 tccggcaatt tgcttttatc cagcggctgg aaaactcccg ctgtcagctg gcgctcaaga
     6421 aagcttgctg acggcaccac cagatcaaag ccggtgctcc ccgccattaa tttgccttcc
     6481 agtacttcgt tagaatcgaa aacatcgtag acgactttaa taccggtttc tttttcaaag
     6541 ttggccaccg tgtccggggc gatataatca gaccagttat aaatgtggag tgttttttgt
     6601 tcagccgcga gcgtgctgac agagacggcc atcagagccc ccgcaaccag acccgatagc
     6661 cattttttat ttaaggcggt catatctctt tccttctgaa aattcgttaa caaacgaaca
     6721 aaagttgtca cactgaaaga tatgcaataa acgtgcatat ttttatcggc gcggtgtaag
     6781 aaaagagaac tctctcccgg cctgcactgc tcactatata aaagcatcgc aagaataact
     6841 ttagccagag tttgagacta tagctcggat taaaaaacgg tatttaacaa atttttatgc
     6901 gtatttgctc tatgtgataa agccaaatcg gcacaagaga agtgaattaa tctttaataa
     6961 aaggttaaaa acagcggata attttgcggc acgcagtcac tgcggcagcg actgcgtgct
     7021 cgaaaggagt tatcagtgga ggaaatagtt ttcggtaacg ttattttgcc cttcatcgtc
     7081 agtcggtaac agcagttgat gggcattagc ttcaagaatg accatcgaaa tctgctcttc
     7141 gctctgacgc acgaaccatg caaactgctc ataggttacg ccctgcataa cggataaaga
     7201 ctggcaaacc accagctttg gcagattatc atcctgcata tcaagaaatg ctttcacggt
     7261 cagcgaactg gcattgatgg ctgataaatc cgccgccagc ggcaatacgg ctgaaggtcg
     7321 gacttccgcc atggcagaaa acaggatcgt gttatcgatc agatcgattt tagcatcaaa
     7381 gacaccgtcg aaattctgca tatggggcag atgcagagcc tgacagttat cacattcaaa
     7441 aaaactcatc cccaggtcat cgagccattg acgcaacgta tccagaccag gaacgaccag
     7501 cgatgtcata attttgtaca acctcttccg ataaaaagac cggcacagct tacgcaaaaa
     7561 gcgcaggcaa aaccatgatc agtaatgtga ttgcgactaa ccacccgttt tcaggcaata
     7621 ttctgtcgta gcgtggcgtt cgatccagcg gatcatttta cccgcgatgt cgataccggt
     7681 ggttttttct attccttcca gccctggcga cgcattcact tccatcacca atggcccgcg
     7741 attagcacgc agaatatcca caccagcaac gtccagcgcc atcgttcgcg cggcttttat
     7801 cgcgatttca cgctcctgtg gtgtgatact ggcgacgctt gccgcgccgc cgcgatgcaa
     7861 attggaacga aaatcgccct ctttcgcccg ccgttcaatc gcagcgacca cttcatcgcc
     7921 aacaaccaga cagcggatat cgcacccttg cgcctctttg atatattcct gcaccagaat
     7981 atgcgcgttc agaccgcgga aagcgtcaat cacgctttcc gccgcctgac gcgtctccgc
     8041 cagcacgacg ccaattccct gcgtgccttc aaccagcttg accaccagcg gcgcaccacc
     8101 gaccatgtcg attaaatcgc tggtatcatc cggcgaatgc gcaatgcccg tgacaggcag
     8161 gtcgatgccc tgacgcgcca gcagttgcat ggaacgcaat ttgtcacgcg cccgggcaat
     8221 ggcgaccgac tcattgagcg gatagctccc cagcatctcg aactggcgca gtgccgccgt
     8281 cccataaaag gtaatggcgg tgccaatacg cgggatcact gcgtcaaaat ggggtaactt
     8341 gcggcctttg tagtgaatag aagacgccgc aggatttatg ttcatgtagc aagaaagcgg
     8401 atcaagaatt tcaaccaggt gaccgcgctg tatagcggct tcacgcagcc gcttacacga
     8461 atagagcgtt ccatcccggg acaatatggc aattttcacc ctgcacctct ctgtcagacc
     8521 tggtcaaaag cccgcgtatc atacatcgac gtggcagttt tagcgcgtcg cccaaccctg
     8581 tttgtgcaaa taatccagaa taaatgggcg gctttcttta atgattgttc ggcggatatg
     8641 atcgctccag gtatcccggc gattattgct gccacgggtg aggtaatatt ccgccagttg
     8701 ctcgtcatac tgcgccagtg cgtctttatc cagcggttga tagctgtttt catgcaccaa
     8761 aatggaggcc ggtaaacgcg gcttaagatc cggattatcc gcaggccagc caaggcacag
     8821 cccaaacagc ggcagaacat gctgcggtaa tttcagcagt tccgtcaccg cttcaatatt
     8881 attgcgcagg ccgccgatat ataccccacc caatcccagc gattccgctg cggttaatgc
     8941 attctgcgcc atcattgccg tatcaacgac accgagcaac agttgttccg ccaggccgag
     9001 ctgagcatcc ggacagatct gtaaatggcg gttaaagtcg gcacagaaca cccaaaactc
     9061 cgccgcttgt gctacgtgtt tttgcccgcc ggtcagcgtc accagttctt cacgtaacgc
     9121 tttgtcggta atgcgaataa tgctactgca ctgcaaaaaa ctggaactgg acgtcgcacg
     9181 ggcgctgtta ataatcgcct cacgctgcgc ttcggaaatg ggttcatcag tgaaatggcg
     9241 aatggagcga tggccacaaa taagttcaat ggttggcgtc attatctttt tctctttctg
     9301 aacgtgaata ttgcggtgga tggttcatca gctgtggggc aagacgtttt gccacctgaa
     9361 gaataaccac caccgcagcg ggaatcatga gcaaaacacc gagaaaaatc atcagaatct
     9421 gcacttctgg ccgagaaaat ggctcaggta gcgacaggga gtcgcttacc gacagcagcg
     9481 ccaccgccag caacatcatt ccgataaatt ccagtatcaa cacgctttta ggcaatttac
     9541 cgatcgcgcg catacgcttc cctctgcaaa gtgagccttc agtctaaaac ttttcactgc
     9601 attgtgttta acagttatag cttttagcaa ttaatgcaac aggttaaacc tactttcagc
     9661 gaatacattt tagcgtgatc attacaggca taaatctatg aggagagaaa taatgcaaac
     9721 cgttattttt ggtcgtccgg gttgccctta ctgtgtgcgt gcaaaagatc tggccgagaa
     9781 attgagcaat gagcgcgatg atttccagta tcagtatgta gatattcgtg cggaagggat
     9841 cactaaagaa gatctacaac aaaaggcagg taaacccgta gaaaccgtgc cgcagatttt
     9901 tgtcgatcag caacatatcg gcggctatac cgattttgct gcatgggtga aagagaatct
     9961 ggacgcctga tcgtctgaca agccctcgcg ttgagggctt tattgatttt ttctgtgctg
    10021 tggtttaaac aaactactga taaataagaa acacaatgcc cccagcgcac accagaacac
    10081 cgcacttaat aaccatgcca gctcttgcca gaatgagcgc gtcggtgaaa aaaacagccg
    10141 cataatgagc atcgaacagg gtgccgccag cattgcgcca aacagaggtt tcaggacttc
    10201 tctacgatgt gaaaagaagc tggcaactgc accaggaaga atgaaaaata gcaaaccgat
    10261 ttcaggatgc ccggcagccc gaaaagcgcc tttcatgtgc gtcgccagaa aaagacacac
    10321 cacaatgaag aggacaaaac agcagattgc ccccgcccaa cgttgtttat gtttcactcg
    10381 ttcctcctga cactgcgtct atcgaacaca tttttcgcca gtgtggcgtt cagtaagata
    10441 aagccgcttc gcattccatg ctaatatagg ccaacgcaat tcatatagcc gttgatacct
    10501 aatgtgatta cactagtaaa atatattgtt actttactat cgtttaggtg cgctgaatga
    10561 atctgcgccc tgaattctgg taaaaaacat tatcgtaaat taccatttct ttcaacagct
    10621 tactagtaaa caagaagtta gcctccgtga atataaacgt cgccgaattg ttaaatggga
    10681 attacattct gttattattt gtggtcctcg cgcttgggct atgtctcggg aaattacgac
    10741 ttggttcgat ccaactgggt aattccattg gcgttttagt cgtatcgctg ttattaggcc
    10801 aacaacattt cagcattaac accgacgcgc ttaatcttgg ctttatgctg tttattttct
    10861 gcgtcggggt cgaagccgga ccgaactttt tttctatttt ttttcgcgat gggaaaaatt
    10921 acctaatgtt agcactggtg atggttggca gtgcgctggt gatcgcctta gggttaggta
    10981 agctgtttgg ctgggatatt ggcctgacgg ccggtatgtt agctggctct atgacgtcga
    11041 caccggttct ggtcggtgct ggcgatacac tgcgtcattc cggcatggaa agcaggcaac
    11101 tctcactggc actggataat ctgagcctcg ggtatgcctt aacctattta atcggtctgg
    11161 tgagtttgat tgttggtgcg cgttacttgc cgaaattgca gcatcaggac ttacagacca
    11221 gcgcccagca aatcgcccgc gaacgtggcc tggacactga tgccaaccgt aaggtttatt
    11281 taccggtgat ccgcgcctac cgcgtcggcc cggagctggt ggcctggacc gacggcaaaa
    11341 atctgcgtga actgggtatt tatcgacaaa ctggctgcta cattgaacgt attcgacgta
    11401 acgggattct ggcgaatcca gacggtgatg ctgtgctaca aatgggcgat gaaatagcgt
    11461 tggtaggcta tcccgacgcc cacgcacgac tcgatcccag cttccgtaac ggcaaagaag
    11521 ttttcgatcg tgaccttctc gacatgcgta tcgtcactga agaagtggtc gttaaaaacc
    11581 ataacgccgt gggcaaacgt ctcgcacaac tgaagttgac cgatcacggt tgcttcctta
    11641 accgcgtcat tcgtagccag attgagatgc cgatagatga caacgtcgtg cttaacaaag
    11701 gtgacgtttt acaagtcagc ggcgatgccc gtcgcgtaaa aaccatcgcc gatcgcatcg
    11761 gctttatctc gattcacagc caggtcactg acttgctggc attctgcgcc ttctttgtta
    11821 ttgggctgat gatcgggatg atcaccttcc agttcagcac attcagtttc ggcatgggga
    11881 acgctgccgg gttgttattc gccggaatta tgctgggctt tatgcgcgct aaccacccga
    11941 ccttcggtta cattccgcag ggtgcattaa gcatggtgaa agagttcggc ttaatggtgt
    12001 ttatggcagg cgttggtctg agcgccggta gcggtattaa taacggcctg ggcgcgattg
    12061 gcggtcagat gttgattgcc ggattgattg tcagtcttgt gccagtggtt atctgtttct
    12121 tgttcggtgc ttatgtattg cgaatgaacc gcgcactgtt gttcggcgca atgatgggcg
    12181 cacgcacctg cgcgccggca atggagatca tcagtgatac agctcgcagt aacatcccgg
    12241 cgctgggcta tgcgggcacc tatgcaatcg ccaacgtcct gctgacgctg gcagggacaa
    12301 tcatcgtcat ggtatggcca ggattaggat aaaactgaag ttgccctgaa aatgaaattt
    12361 tttttgcaca accgcagaac ttttccgcag ggcatcagtc ttaattagtg ccactgcttt
    12421 tctttgatgt ccccattttg tggagcccat caaccccgcc atttcggttc aaggttgatg
    12481 ggttttttgt tgcctgaaat ttatctacta cccagcaatc ctctcaacca tcctcaaaat
    12541 ctcctcgcgt gatagcggct tacggtcggt gacaaaatgc agcgtcatcc cctcaataaa
    12601 cgcatcaagc gcgcgggcgg ttccgggttc aaaccattgt tcgagcgttt gttgactgcg
    12661 ctgcatccag ttttgcatta ccgtttttaa tagcggtttt cggctagcca gcgcgtagag
    12721 ctggtacatc agctccatat tatccggcgt tgcgacctgt gagctgtaga tcatatcggt
    12781 gatagcctgg catgcgcctg gagcatcact aacatcgcta aaaaatgcct gatattgccg
    12841 ggacatgatc tcagtaaaac ggctgaacgc ctccagcaac aactcatcaa ttcctgaaaa
    12901 atagtaggtc atcgatccca acggcacccc ggcaagggta gcaattttgc ggtgtgtaac
    12961 agcatgtatt ccgtaaagtt tcaccgcctc cagcgtggcc tggataattt tttctcgccg
    13021 ttgcggatcg ttagcgcgac gcatgtcatt tcctctctct gcaattgtgt acaaatgtac
    13081 acagtcttgc taatgttgtc ttccctcttc ttattttgac gtggtctatg accgtaaatt
    13141 cttcacgtaa tgcattgaaa cgccgaacct gggcgctgtt tatgttcttc tttttgccag
    13201 gcctgttaat ggcgtcctgg gcaacccgta cgcctgctat ccgcgatatt ctctctgtct
    13261 cgatcgctga aatgggcggt gttctctttg gtctgtcgat cggttcgatg agcggtattc
    13321 tctgctcggc gtggttagtg aaacgctttg gcacgcgtaa tgtcatcctg gtcacgatgt
    13381 cctgcgcatt gatcgggatg atgatattaa gtctggcact ctggctaaca tcgcccctgc
    13441 tctttgccgt tggtctcggc gtctttgggg caagttttgg ttctgcggaa gtggcgataa
    13501 acgttgaagg tgctgccgtt gagcgagaaa tgaataagac ggttttgccg atgatgcacg
    13561 gtttttatag cctgggcacg ctggcaggcg ctggtgtcgg gatggcactg acggcctttg
    13621 gcgttccggc aacggtgcac attttattgg cggcgctggt aggcatcgcg cctatttata
    13681 tcgccattca ggcaatccct gacggtacgg gcaaaaatgc tgccgatggc acccagcatg
    13741 gcgaaaaagg cgttcctttt tatcgcgata tccagttgct gttgattggt gttgtagtgc
    13801 tggcgatggc ctttgccgaa ggttctgcca acgactggtt acccttatta atggttgatg
    13861 gtcacggttt tagccctact tccggctcgc tgatttatgc cggttttacc ctggggatga
    13921 ccgttggacg ctttaccggc ggttggttca ttgaccgtta cagtcgcgtt gccgtggttc
    13981 gggccagtgc actaatgggg gcgttgggta ttgggctgat tatttttgtc gatagcgcct
    14041 gggttgctgg ggtgtctgtt gtactttggg gactgggtgc ctcgctgggc ttcccgctga
    14101 ccatttctgc cgccagcgat accggccccg atgcaccaac ccgcgtcagt gtggtagcaa
    14161 cgaccggtta tctggctttc ctcgtcgggc cgccgctgct gggctatctc ggcgaacatt
    14221 atggattacg tagtgcaatg ctggttgtac tggcgctggt tattctcgcg gctattgtcg
    14281 cgaaagccgt cgccaaaccc gataccaaaa cgcagacggc gatggagaat agttgatgag
    14341 cattaaatta attgcggtag acatggatgg tactttctta agcgatcaaa aaacctataa
    14401 ccgtgagcgg tttatggctc agtatcagca aatgaaagca caaggcattc gctttgtggt
    14461 cgccagcggg aatcaatatt atcagttgat ctctttcttc cctgaaattg ctaatgaaat
    14521 agcctttgtg gctgaaaacg gcggctgggt agtgagcgaa ggcaaagatg tttttaatgg
    14581 cgagctgtcg aaggatgcgt ttgctactgt cgtggaacat ttgctgacgc gcccggaagt
    14641 ggaaattatt gcctgcggaa aaaatagcgc ctataccctc aaaaaatatg acgatgccat
    14701 gaaaacggtg gcggaaatgt attatcaccg tctggaatac gtcgataact ttgacaactt
    14761 agaagatatc ttctttaagt ttggtctgaa tctttccgat gaactgattc cacaagtaca
    14821 aaaagcatta catgaggcca tcggcgatat tatggtgccg gtccacaccg gcaacggcag
    14881 catcgatctg attatccccg gcgtacataa agccaatggc cttcgccaac tgcagaaatt
    14941 atggggaata gacgacagcg aagtggtggt ctttggcgat ggcggtaacg atattgagat
    15001 gctgcgtcag gcaggcttta gttttgcaat ggaaaatgcc ggcagcgcgg tcgtcgcagc
    15061 ggcaaaatac cgggcaggct ccaataaccg tgaaggcgta ctggatgtga tcgataaagt
    15121 gcttaaacac gaagcgccat ttaaccaata aaacggcagc acgcccggca acacaatgcc
    15181 tgaatgaaga ttgcataatt cgcggaaatt aacccgctgc attatttcag gcgaaatgat
    15241 tatgaaaaat tgtctactgt tgggcgcgct tttaatgggc tttactggtg tggcgatggc
    15301 gcaaagtgtc accgtcgatg tgccgagtgg atacaaagtg gttgtggttc ctgattcagt
    15361 cagcgttcca caagcggtca gcgtcgcgac tgtgccgcaa accgtctatg tggctcctgc
    15421 gccagctccg gcttaccgcc cgcatcctta tgttcgccat cttgccagcg taggtgaagg
    15481 gatggttatc gaacaccaga ttgacgacca tcaccattaa ttcgagaatg cctgatcgca
    15541 caaagcaatc aggcattttt ttacccttcg tgagaatttc ccatctgttt atcttttaaa
    15601 aagataacca tcagcgacag ccacaaaatg ccgttgacaa ggttgaagag attaaacagt
    15661 ccgttgcccc cgttcaacca ggcatgtttg ctgatttcaa taccgacggt aaagattagc
    15721 atttgcagca tccccatcgc cgcagaaacc gtacctttac tcatatcgct ggcaaacagg
    15781 gttaatcgca ccagtcccgc attcgccaga ccaataccga aagcataaat acttaacccg
    15841 gcggtcatcc ataaatacgc gtgcgatgag ataaccgttg ccgcagcagc gaccaacaga
    15901 ccaatcataa tcggccagcc acccataata atcagcgaac gtacggtgcg gcgcgaggtc
    15961 agacgcgcta acagcaagtt acccgcaatt aacgccccga aaataggcac ttgcagcaag
    16021 ccatattcat agctgctcaa ctgctcaccg gtaataatga taatcggcga ctgggcgatc
    16081 cacgccaaca acggcaggct aacgaatccc agcgccagcg cccccgccac aaagcggccg
    16141 ttcttcagca ccagcttata gtcacgaccg agttctttca gcgacagttt ctcgcctata
    16201 cgcgtggcgg tttcaggcat agctcgttgc agaccgaaaa aggagatcgc tgccaatgcg
    16261 gcaaacaaga caaacatccc ttcccagggc agcacatgga tccacgccgc gcccaccagc
    16321 ggaccaagta gtggagcaat cagcgccacg ttcgccatta gcgcggtgat cttgatacaa
    16381 accgcctctt cgaaggattc ctgaattgcg gcgtatccca cagcgccaat gaaacagagg
    16441 cttataccct gcaagaagcg caacagggtg aattgttcaa tgttttgtgc cagtaatatt
    16501 gccagacagg tgacgataaa ccacaccact cccgccagca tcaccggacg gcggccaata
    16561 cgatccgaca acggccccag gagccattgt aaaaacatcc cgcccgccag atacgcagtc
    16621 atcgaagtag gaacccaatc aatgcccgcc tgatattgtt ccaccacggc caacataccg
    16681 ggttgaatca tatcgttgcc gatataggtt gaaaattcgt aaagcaccag acagagaggg
    16741 aaaagtaacg cctgacgtcc aagcctgaca ccggaagcta atttattttg catgcaattt
    16801 cttcgccaat aataatcgcg caaagtttaa taaaagcgca gctaacgaga aagcgaattt
    16861 tgtagctgaa accacggtta agcacattct tacattattg cgagtatagc tacgctttct
    16921 ttaagtttta tttaaccttt gcccgttaca atcacctacc gtaaacaggc cgcttgaggg
    16981 aaataagacg atgccgcttt acccagttta acctgcactt tattctcaac gacttgcctg
    17041 tattggctcc cttttaatca ctttgcgtcg ggaagttatg ctggaaaatt tgaatctctc
    17101 tctattctct cttattaacg caacgccaga ctctgcgccg tggatgatct cgttggcgat
    17161 ttttattgct aaagatttga ttaccgtggt gccgttgctg gccgcagtac tttggttgtg
    17221 ggggcttaca gcacaacggc aactggtgat aaaaatcgct atcgcgctgg cggtcagcct
    17281 gtttgtgtcc tggacgatgg gacatctttt tccgcacgac cgaccctttg tcgaaaatat
    17341 cggctataac ttcctgcatc atgcggcgga tgactcattc ccgagcgatc acggtacggt
    17401 gattttcacc tttgcactgg catttttatg ctggcatcgc ctgtggtccg gctcactttt
    17461 aatggtgctg gccgtcgtca ttgcctggtc gcgcgtttat cttggcgtcc actggccgct
    17521 ggatatgctc ggtggattgc tggcaggtat gattggctgc cttagcgccc agattatctg
    17581 gcaagcgatg gggcataaac tctatcaacg tctgcaatcg tggtatcgct tctgttttgc
    17641 attaccgatc cgcaaaggct gggtgcgtga ctgatctctg ctaaaaagtg tagtattgag
    17701 cggctcgctt caataactat tcagagggat tatggaaaca cgtcgcgaag agcgtatcgg
    17761 gcagctgctg caagaattaa aacgcagtga taagttacat cttaaagacg ccgccgccct
    17821 gcttggggtt tcggagatga cgattcgtcg cgatctgaac aaccacagtg cgcccgtcgt
    17881 tttgctcggc ggctatattg ttctggaacc acgcagcgcc agccattacc tgttaagcga
    17941 tcaaaaatcc cgcctggtgg aagaaaaacg ccgggcggca aaactggctg cgacgctggt
    18001 agaacccgat cagaccctct tttttgactg tggcaccacc acgccgtgga ttattgaagc
    18061 aattgataat gaaatccctt ttaccgccgt ttgttattca ctaaatacct ttctggcgct
    18121 gaaagagaaa ccccattgcc gcgcgtttct ttgcggtggt gaatttcacg ccagcaacgc
    18181 cattttcaaa cccatcgatt ttcagcaaac gctgaataat ttttgcccgg atatcgcttt
    18241 ttattccgct gcgggcgtgc atgtcagtaa aggcgctacc tgctttaatc ttgaagagtt
    18301 gccggtaaaa cactgggcca tgtcgatggc gcaaaagcat gtgctggttg tcgaccacag
    18361 taaatttggc aaggtgcgtc cggcgcgcat gggtgacctg aaacgctttg atattgttgt
    18421 gagcgattgt tgcccggaag atgagtatgt gaagtacgcg cagacgcagc gtattaagtt
    18481 gatgtattaa tgacgtataa ccggatgacg tttcgcgcca tccggttatc agaagattaa
    18541 gagaaccagc tgccgaacca ctgatggaat ttcatcatca cgaaatccca catccgacca
    18601 aagaatccgc cctcttccac attttccatc acgatcagcg gacgctgctc aatggattta
    18661 ccgttaagct ggaaatcaat ggtgccgaca acctgacctt ttttcagcgg tgcggtaagc
    18721 tgcggttccg ttaacgtata actcgccttc aggtttttca gctgcccacg cggtatggtc
    18781 actgaacccg cttcgcctgc cccgagattc acttcgctct tatcaccaaa ccagacgcgc
    18841 tgagtcacaa atgtggcatc aggtttaatt ggcgtcacgg tttcaaagaa acggaagccc
    18901 caggtcaata atttctcaga ctcattaaaa cggatacggt cagttttcgc ccccagcact
    18961 acggagatta aacgcatatc gccctgggta gccgaagcaa ccagattata tcccgcgcct
    19021 gccgtggttc ctgtcttcat gccatcaaca ttcagattgc tgctccacag cagacgatta
    19081 cggttaggct gacgaatttt gttgaaggtg aactctttct ctttatgaat ggcgtactct
    19141 tccggcacat cgtggatcaa tgctttaccc agcaatgcca tatcgcgcgc ggtactgaac
    19201 tgccccggcg catccaggcc gtgcaccgtc tggaaggtag tgttggtcag acccagtttt
    19261 ttggcataac cattcatcaa accaataaat gactcctggc tcccggcaac gtaatcagcc
    19321 agcgcaatac aggcgtcatt accggactgg ataatcacac ctttgttcaa gtctgccacc
    19381 gaaacctgat cgcccggttt gaggaacatt accgatgaac cacgcagtgc cggatttccc
    19441 gtcgcccagg catctttacc gaccgtcacc atatcggtga gtttaatctt atcggcctta
    19501 agcgcctgcc caaccacata gctggtcata attttagtca ggctcgcggg atccagtttc
    19561 tcatccgcat tgccttctgc cagcacttta ccgctggcgt aatccattaa aatccatgca
    19621 cgcgcatcca cgctcggcgc ttcaacggtt tgttccgccg cgaatgccgt tggggcaaaa
    19681 aggaataaaa atgcagaacc cgctgcaaga ccacgaagga gagaggagta ttgcgtcata
    19741 aatgccaccc gagtatccat tcaaaaaaaa tacgttatat cgccgtatga taagttcggt
    19801 gattaataac gcacacacaa acttaaagaa accgtaagtt ggtaaagttt ttaaagttta
    19861 tgcaatatcc tgccgcttcg ctgcttctgg tgtcattttt ttgccagctg ttgcttaaaa
    19921 gttaacttta cttcaccatc ggagtcctgt tcagcctgtg gctgcctgtc ggaaaagggg
    19981 tgagtatgat tacgctgtgg ggtcggaata attcaaccaa cgtaaaaaaa gtattgctga
    20041 cgctcgaaga actggaacta ccttatgagc aaattctcgc gggccgtgag tttgggataa
    20101 atcacgatgc tgattttctg gcgatgaacc ctaacgggct ggtgccgttg ttgcgtgacg
    20161 acgaaagtga tctcattctt tgggaatcaa acgccattgt ccgctatctg gcggcacagt
    20221 acgggcaaaa acgcctgtgg atcgactcac cggcacgtcg tgcggaagcc gaaaaatgga
    20281 tggactgggc aaaccagacg ctcagtaatg ctcatcgcgg gatcctgatg ggattagtca
    20341 gaacaccacc ggaagagcgc gatcaggccg ccattgatgc cagttgcaaa gagtgcgacg
    20401 ccctgtttgc cctgctcgat gcggaactgg caaaagtaaa atggttctca ggcgacgagt
    20461 ttggtgtggg cgatatcgct atcgcaccgt tcatctacaa tttgtttaac gttggcctga
    20521 cctggacacc gcgtccaaat ctgcaacgct ggtatcagca actcactgaa cgccccgcag
    20581 tgcgcaaagt ggtgatgatt cccgttagct aattgcgtgg gctaacttta agtaattccc
    20641 cactggactc gtcggtgaga acgtataaat aaccgtcggg tccagtgcga acatcacgaa
    20701 ttcgctgccc tctgtccgtt aaaatacggc catcttctgt cactttgtcg ccgttgacgc
    20761 tcatcacaat gacatcttta tctttcagcg cgccaataaa taatttttgc tgccactggg
    20821 ggaatttgtc gctgttatag aaggccatgc cgctcacggc gggagaatct ttccagtaaa
    20881 aaacaggttg ctcggtcccg gcgacgatct cccctttagc ttccggtatc ttaaagcccg
    20941 aatagttgat tccccaggtt gccagcggcc agccgtagtt tttgcctttt tgcggaatat
    21001 taatttcatc accaccgcgc gggccatgtt cattcagcca tagtgcatta ctccacggat
    21061 tcatcgccat tccttgcgga ttacgaatgc cataagacca gatctcggcg cgcacaccgg
    21121 attcctttat aaaaggatta tcatccggga tttctcccag gtcggtcaga cgcaccagtt
    21181 tgccctgtaa tttatccaga tcctgcgccg tcgggcgctg attgttttcg cccagagcaa
    21241 taaaaagata acctttaccg tcgaatacca tccgcccgcc aaaatggtta ccggtagaca
    21301 gttttggcat ctgacgaaag acggtgcgga agtcggtcac ttttgagaga tcatcactta
    21361 agcggccata acccacagca gttccggctt tgccatcatc gccaacttcg gaataactta
    21421 accagatgcg gcgcgactga gcaaaatcgg gcgctaaaac cacgtccagc aggccgccct
    21481 gcccgtgcgc ccaaacgtcc ggcactccgg aaagcggcgc agataatcct tttcctgctt
    21541 gccagtgacg caactcgccg ccgcgcagag tgattaacat accgtgatta tcgggtaaaa
    21601 aggccagcgc ccagggatgg tcgagtttgt cttgcagtac ttcgacattt accgttgcag
    21661 gagctgccca gagagcggaa gaaagacaaa taaggggcac aaggaaaaag gattgtcgat
    21721 gcataacgcc tccggattga ctctggctta aagcgtagtc agtggaggag ataacaaatt
    21781 catttttaca aaaacttaaa catgaagggg gagacgcttt ctccccctta gttttcaggc
    21841 cttctcaagc atggcgtgct tctgcaggct ctggatactc agcgttaagc tcatcagaca
    21901 attttcaagc ttatcggcgt tgacggtaat aacagtcggg caatcatggt gcccactcat
    21961 caaacatact gcggctgtcg ctaatgcttc ttcagcatga tgaagagcac tccactcttc
    22021 ctgatccaga tgaagattca accgcagcga tttgtcgtgc agttcgcgat tcagtttaaa
    22081 aaagttatct cgtagatgat tgctttcgct gacggacatg tatccttttg ccttcctcag
    22141 ttgcacgtat gcggcgaggt caacgcgtgc gtcgatcaac ctgttcagca gatcgattcg
    22201 ctgtctgtca acgaacatac ttcgatcctc ctctttccag ccttgttgca actaaatagt
    22261 atggctgttt tttgagcaaa atgctgtcca ggatcaattc ttcacgccga attaacaaaa
    22321 tttacatttc cttcaactcc gccagcgaat gagattgcga tacaaatttt atccagaact
    22381 ctgtagaatc cctgccctga cctttcctgt aactgaatat tttttaagct atgagcaaag
    22441 taactcccca gccgaaaatc ggctttgttt cccttggctg tccgaaaaac cttgtcgatt
    22501 cagagcgtat tctcaccgaa ctacgcactg aaggttatga cgtggtaccg agctatgatg
    22561 atgcggacat ggtgatcgtc aacacctgcg gctttattga cagcgcggta caagaatcac
    22621 tggaagccat tggtgaagcg ttgaatgaaa acggcaaggt aattgtgacc ggttgtctgg
    22681 gggcaaaaga agatcagatc cgcgaagtcc acccgaaagt gctggaaatc accgggcctc
    22741 atagctatga gcaggttctg gagcacgttc atcactacgt gccaaaaccg aaacacaacc
    22801 cattcctgag cctggtgcca gaacaaggtg tgaaactgac gccgcgtcat tatgcctatc
    22861 tgaaaatttc tgaaggctgt aatcaccgct gcaccttctg cattattccg tctatgcgcg
    22921 gcgatctggt gagccgtccg attggcgaag tgttaagtga agcgaaacgt ctggttgatg
    22981 cgggcgttaa agaaattctg gtgatctcgc aggatacttc cgcgtatggc gtcgatgtta
    23041 aacatcgtac tggcttccac aacggcgagc cggtaaaaac cagcatggtc agcctgtgcg
    23101 aacagttatc gaaactgggg atctggacac gtctgcacta cgtttaccct tatccgcatg
    23161 tggacgacgt catcccactg atggcagagg gcaaaatcct gccgtatctg gacattccgt
    23221 tgcagcacgc cagcccgcgc attctcaaac tgatgaaacg tccgggttct gtagatcgcc
    23281 aactggcgcg catcaaacag tggcgcaaaa tctgcccgga actgacccta cgctcaacct
    23341 ttattgttgg cttccctggc gagacggaag aagatttcca gatgctactc gacttcctga
    23401 aagaagcacg tctggatcgc gttggctgct ttaaatacag cccggttgaa ggtgcagacg
    23461 ccaatgccct gcctgaccag gttccggaag aagtgaaaga agaacgctgg aaccgtttca
    23521 tgcagttgca gcagcaaatt tccgccgagc gcctgcaaga gaaagtgggc cgtgaaattc
    23581 tggtgattat cgacgaagtg gacgaagaag gcgcgattgg tcgcagcatg gcagatgcac
    23641 cggaaatcga cggcgcggtt tatctcaatg gtgaaaccaa cgttaagccg ggtgatatcc
    23701 tgcgagtgaa agtcgagcac gccgatgagt acgatttgtg gggtagccgg gtttaattaa
    23761 ttcaggccgg gcaaaatcat ttgtccggcc tgcgaataat gattactgcc agctaatctg
    23821 cgccctgccg tttttcttac tcttatacag ccgctcatcc gccagcattt tcaagcgttc
    23881 gacggtagta cattcatttg aatgcgctac accgatactg gtgcttaaat taatgcttcc
    23941 cacctggaaa tgaacgattt cttttagaat cgatagcaac tcttccagcg tgccgccagt
    24001 ataaatcacc gcaaattcgt cacctccgaa gcggtagact ttacctttat ctaccgggat
    24061 ctgtgattta aggattttca ccactttcat caacacttca tcgcccagca aatgaccgaa
    24121 ggtgtcgtta atgtttttga atttatcgac gtcaaacagg cacaaataac cgcctttttc
    24181 cggcagcgct tttaaatctt cgtcaaaaga ttttctgccc atcgctttgg tcagatcgtc
    24241 caggaatgcc gagttataca tactgtagag ttttttgaca tcggcagccg aaaagaaaag
    24301 cccgccatcc agtacgcgtt caaaatggtt attaataatg gcttctttct ccacaatacc
    24361 acgattaatc actctgcgta ccagatagag gcaactggca cccgatatca ctgccaccaa
    24421 tgcggcaaag aaaataatca atgtacatac ctgaaaataa acgtcttttt tataagcaaa
    24481 tacgtacaac ccggtcggca aataggagaa gtgctccatt gcttccgaaa cggtgaagat
    24541 ggcgttttta gcgttatatt catcaccggt caccgattta ccgttcttga acagggagaa
    24601 catcaggttc tcgctatcca tcgccagcga ttttgacagt aaagaaaaac gttccagacc
    24661 aagcaaaaat atcagactac gattgtgagt taccggatag aggtaataga cgaaggtttt
    24721 accatcaaaa gagataaacc ctgtattttt gatatgtcga tacttaacaa cctgcataat
    24781 atctgcacaa taatttttct cgaactcctt tacctgaata cattcatcac tggaaatcag
    24841 ataaggaccg acatcgtaca aataagaggg tgaaaccgag tcggcgtaag ctgtgcgtaa
    24901 gccaaccaac agctgttcct ccgtaggatt tagcgtgttg atgagatcaa ttgccacact
    24961 acgccgatat tcagcaatat ttgccaccgc aaaagcagta tcaataacgg actgtttcag
    25021 gccatacacc cgttccttta ccatttgagt gtagatcccg caggcaactg ctgaaatcat
    25081 gataaaaatc agcagactga ctgtaagtac gaatttattg attctggaca tacgtaaact
    25141 actcttttac taattttcca cttttatccc aggcggagaa tggcatgggt ttctggtaca
    25201 gatacccctg agcctgaaca catcctaatt tgattaattc acgcgcaacg tcttcgctct
    25261 ctacgccttc cgcaatcacc gggatccggt tatagcgtgc cagatcgaaa ataatgcgca
    25321 caatttttcc tgcagtaccg aaaatatcgc cggtaaattg cgaatccacc tttatactgt
    25381 ctggattaat ttcataaaaa cgtttcaggt ttgacaaacc ggtgccaaag tcatcaatcg
    25441 atacccgtac cccttgttta cgcagttgtt caatattgag atagaaatgt tcattaattc
    25501 gctcgatcac ggtttcggtg atctcaacac aaaggctgtt cgccttcagg ccattttctt
    25561 tcatgatggt agtaaaacgc tcatgaaagt gtggttcgtt taactgccag ggcgaaacat
    25621 taatatgcag taagaaatca tccttcagcg acgcacgctg actaaaatgt ctgaattcac
    25681 ccatcgcggt tttaataatt tgatagccca gctcattgat aaacccgata tcttcagcaa
    25741 tggtaatgaa cttcagcggt gaaataatcc cccgctccgc agacacccaa cggcacaacg
    25801 cctccgcgcc caaaatggtg cgggtgttaa tatcaacaat cggttgatag accaggaata
    25861 gttcgccaat ttccattgca tggcgaatat tacgcgcaat ccagatattc tcttctattt
    25921 cgttgagttt cgcatcatca cagaaaataa actctgagaa catatggttg cggatactgg
    25981 aaatcgccag tttcagcttt tctgcgtaac tccaggcgtt ttccccctgc aaaggttcac
    26041 aaatagcaga cttatggatg cgcagattgc ggtagccaaa actgctgtca aaatcgttta
    26101 ccgtcgctat cttacgggta aacgtctgta cattttcccg gcacaagagt aaatagagat
    26161 cggcattaaa tgtcacgata tcgcagcatt gcgcatattt ctcggcaatt cgctgacaca
    26221 atttcctgac gattttattg cgttcatcaa caccaaacac cgcttccaca gcttccagac
    26281 cacacagttt gatgatcccc acagccagat actgattatt gttggactca ataatcgcca
    26341 tatttttaat aaaaccgctt tcctgatcgt aagagatcag cgagagcaat ttatttgccc
    26401 gttcaatttc agcatctttc atttccgcga tcgaattttt gatgctgata atctctttcg
    26461 catcgccttc acgaatcttc agggcctcga tttgatccgt agagtcaggc aaaaacgcca
    26521 gcgtattgac caaatctgtc atttcagaaa attgccgacg gaacactcga gtggtcatta
    26581 aataaaccac cagcaatacg ataataatga aagcaaacgg cagataaaaa agtttatctg
    26641 atgcgttcaa taaattacca ccaggataat aaaaatagat ttcgaata
//
LOCUS       DAZFFE010000062        26447 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFFE010000062 DAZFFE010000000
VERSION     DAZFFE010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.062"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..195)
                     /locus_tag="ACHJ69_002776"
     CDS             complement(<1..195)
                     /locus_tag="ACHJ69_002776"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO5015727.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEI"
     gene            complement(227..496)
                     /locus_tag="ACHJ69_002777"
     CDS             complement(227..496)
                     /locus_tag="ACHJ69_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO5015728.1"
                     /translation="MLTKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTALFRDRFGEAE"
     gene            complement(486..1994)
                     /locus_tag="ACHJ69_002778"
     CDS             complement(486..1994)
                     /locus_tag="ACHJ69_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO5015729.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQRTDITLASRTLCLDVRKGEWSAKAAALLHVPCSAFAPLVQPGEHAGWV
                     SESLCKTLGFSQSVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAVSLNRGYADYLA
                     GNWSLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLNIDMLISSVSLGLTMEFANC
                     FACFNVPGTSVLKVIGPATHNPLWLQLKADILQRPVEAIAFSEAVSVGALLTAAPDIP
                     PPQVTIAQRLLPNRARYHQLQRYQHKWESWYQLKLQQEGVMPLHHREEHYVD"
     gene            complement(1987..3345)
                     /locus_tag="ACHJ69_002779"
     CDS             complement(1987..3345)
                     /locus_tag="ACHJ69_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO5015730.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNIIAIGLVPVIALVLYGIFRMTRSTV
                     YA"
     gene            complement(3419..3700)
                     /locus_tag="ACHJ69_002780"
     CDS             complement(3419..3700)
                     /locus_tag="ACHJ69_002780"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO5015731.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKK"
     gene            complement(3697..4170)
                     /locus_tag="ACHJ69_002781"
     CDS             complement(3697..4170)
                     /locus_tag="ACHJ69_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO5015732.1"
                     /translation="MQGIQFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMGGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMQALLTMETTEIHTVILNKMNERGEI"
     gene            complement(4195..4941)
                     /locus_tag="ACHJ69_002782"
     CDS             complement(4195..4941)
                     /locus_tag="ACHJ69_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5015733.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LEVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIEPANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIV
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRELPVEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(5140..5541)
                     /gene="nikR"
                     /locus_tag="ACHJ69_002783"
     CDS             complement(5140..5541)
                     /gene="nikR"
                     /locus_tag="ACHJ69_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO5015734.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(5547..6353)
                     /gene="nikE"
                     /locus_tag="ACHJ69_002784"
     CDS             complement(5547..6353)
                     /gene="nikE"
                     /locus_tag="ACHJ69_002784"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO5015735.1"
                     /translation="MTLLNVSDLSHHYAHGGFSSKHQHQAVLNNVSLALKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLMLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(6350..7114)
                     /gene="nikD"
                     /locus_tag="ACHJ69_002785"
     CDS             complement(6350..7114)
                     /gene="nikD"
                     /locus_tag="ACHJ69_002785"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO5015736.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(7114..7947)
                     /gene="nikC"
                     /locus_tag="ACHJ69_002786"
     CDS             complement(7114..7947)
                     /gene="nikC"
                     /locus_tag="ACHJ69_002786"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO5015737.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRMSLGSVMACLLLVLTLGLIIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(7944..8888)
                     /gene="nikB"
                     /locus_tag="ACHJ69_002787"
     CDS             complement(7944..8888)
                     /gene="nikB"
                     /locus_tag="ACHJ69_002787"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO5015738.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLSD
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(8888..10462)
                     /gene="nikA"
                     /locus_tag="ACHJ69_002788"
     CDS             complement(8888..10462)
                     /gene="nikA"
                     /locus_tag="ACHJ69_002788"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5015739.1"
                     /translation="MFSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALGKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(10573..11160)
                     /gene="acpT"
                     /locus_tag="ACHJ69_002789"
     CDS             complement(10573..11160)
                     /gene="acpT"
                     /locus_tag="ACHJ69_002789"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO5015740.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYNSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(11215..12264)
                     /locus_tag="ACHJ69_002790"
     CDS             complement(11215..12264)
                     /locus_tag="ACHJ69_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO5015741.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPRFNKELT
                     RKLFKLQEMLPFLNLHMSPERILQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGIIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            12363..13613
                     /locus_tag="ACHJ69_002791"
     CDS             12363..13613
                     /locus_tag="ACHJ69_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5015742.1"
                     /translation="MKHCCKNVVILMPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGVVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFLSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFV
                     GTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFP
                     ALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVA
                     IALLLTWRLKKRPPVEIPEAASSS"
     gene            complement(13617..14174)
                     /gene="dcrB"
                     /locus_tag="ACHJ69_002792"
     CDS             complement(13617..14174)
                     /gene="dcrB"
                     /locus_tag="ACHJ69_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO5015743.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADNQQKAQTTAENIINTLVIQ"
     gene            complement(14247..14912)
                     /locus_tag="ACHJ69_002793"
     CDS             complement(14247..14912)
                     /locus_tag="ACHJ69_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO5015744.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRHWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            15133..15378
                     /gene="tusA"
                     /locus_tag="ACHJ69_002794"
     CDS             15133..15378
                     /gene="tusA"
                     /locus_tag="ACHJ69_002794"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO5015745.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(15647..17845)
                     /gene="zntA"
                     /locus_tag="ACHJ69_002795"
     CDS             complement(15647..17845)
                     /gene="zntA"
                     /locus_tag="ACHJ69_002795"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO5015746.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNTNDCCCGGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQASDEPQESRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGTTSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVCNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRK"
     gene            complement(17919..18545)
                     /locus_tag="ACHJ69_002796"
     CDS             complement(17919..18545)
                     /locus_tag="ACHJ69_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO5015747.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            18686..19045
                     /locus_tag="ACHJ69_002797"
     CDS             18686..19045
                     /locus_tag="ACHJ69_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO5015748.1"
                     /translation="MSKPPLFFIVIIGLIIVSASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(19048..19317)
                     /locus_tag="ACHJ69_002798"
     CDS             complement(19048..19317)
                     /locus_tag="ACHJ69_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO5015749.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(19307..19903)
                     /gene="rsmD"
                     /locus_tag="ACHJ69_002799"
     CDS             complement(19307..19903)
                     /gene="rsmD"
                     /locus_tag="ACHJ69_002799"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO5015750.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            20053..21549
                     /gene="ftsY"
                     /locus_tag="ACHJ69_002800"
     CDS             20053..21549
                     /gene="ftsY"
                     /locus_tag="ACHJ69_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO5015751.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQIPEKETEVQNEQPVVEEIVQAQEP
                     VKASEHAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
                     AAEAAEEAVMVVSPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            21552..22220
                     /gene="ftsE"
                     /locus_tag="ACHJ69_002801"
     CDS             21552..22220
                     /gene="ftsE"
                     /locus_tag="ACHJ69_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO5015752.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            22213..23271
                     /gene="ftsX"
                     /locus_tag="ACHJ69_002802"
     CDS             22213..23271
                     /gene="ftsX"
                     /locus_tag="ACHJ69_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO5015753.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEYRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            23516..24370
                     /gene="rpoH"
                     /locus_tag="ACHJ69_002803"
     CDS             23516..24370
                     /gene="rpoH"
                     /locus_tag="ACHJ69_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO5015754.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            24641..25744
                     /gene="livJ"
                     /locus_tag="ACHJ69_002804"
     CDS             24641..25744
                     /gene="livJ"
                     /locus_tag="ACHJ69_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO5015755.1"
                     /translation="MNMKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(25932..26315)
                     /gene="panM"
                     /locus_tag="ACHJ69_002805"
     CDS             complement(25932..26315)
                     /gene="panM"
                     /locus_tag="ACHJ69_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO5015756.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNYRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPDVSCWWMADAGV
                     EDRGVMTAFMRALGFTAQQGGWEKH"
BASE COUNT         6416 a         6905 c         7114 g         6012 t
ORIGIN      
        1 gatttcatat atgtaatcgt atcccgacag ttgtgcattg gtgggcgcaa tctgaatgaa
       61 gttgggtaat cccgaacgct caatagcgtt taaaattgaa attgtcgtct ccagattggt
      121 ggtattaaac gcgcccgcta atacccgttt ttcctgaatg cggtcgagca gtatccgtcc
      181 attcactaat ggcatattga aaacctcatc ttttaatatc agttaattat tcagcttcac
      241 cgaagcggtc gcgaaataag gcggtgagag tctgtatggc ctcgttttca tcatcaccgt
      301 cgcagcgtat ttccaccgtt tttccgcctt ttattccggc gcgcataata ttcatcatac
      361 tggtggcatt aatatcatta ccctcataat gcagcgtcac tttacagcta tactttttgg
      421 cggctttcgc cagacaggcc gccggacgtg catgcaggcc agtactgttt ctgacctcca
      481 cggttttagt caacatagtg ctcctcccga tgatgtaatg gcatcacgcc ttcttgctgt
      541 aatttcaact gataccagct ttcccatttg tgctgataac gctgtaattg atgatatctt
      601 gcccgattcg gtaacaaacg ttgggctatg gtaacttgcg gcggcggaat atccggtgcc
      661 gcggttaata aggctccgac agaaaccgcc tcgctaaatg caattgcttc aaccggacgc
      721 tgtaaaatat ctgcctttaa ttgcagccaa agggggttat gagtcgccgg accgatcact
      781 tttaacacgc tggtgccagg cacattaaaa caggcaaagc agttggcaaa ttccatggtt
      841 aatcccagtg agacgctgga aattaacatg tcgatattta acgtatcgcc aagcccataa
      901 aataatccgc gggtgtggcg atttttatag ggtgaacccg aaccgcgaag atgcggaata
      961 aagacaggaa tgtcatcaag cgaccagttc cccgccaggt aatccgcata cccacgattg
     1021 agcgacacgg cgatctcctc atcggttagc cggaacgtgt tacgcagcca ctccagcgcg
     1081 aaaccgccca cgggcagaga agcaaacagg gtgaactggt tggcaagcga gtaacagccg
     1141 tttgccagct tgtcacgttt ggcctgttca tccagcgtcg gttgtgtatc cagttgcaga
     1201 atgccttccg tggtgcccgt cgagttaagg atatcgcccg gcatcatctg caacgcccgc
     1261 gcacccacca tatggtcatg tccggccagc gtcacgctga ccgattgcga gaacccaagc
     1321 gttttacaga gcgactcact gacccatccg gcgtgttcgc ctggctgcac caatggcgca
     1381 aatgctgaac agggaacgtg taacaacgcc gccgctttcg ctgaccattc gcctttgcgc
     1441 acgtccaggc acagtgtacg gctggctaag gtgatatcag ttcgctgttc gccagtcagc
     1501 cgccagagca gcacttccgg tgcatgtagc cagcgcaggc cgcgacgatt gcgcagcgga
     1561 taatgctcca gcaaccaacg cattttgaag gcagagtaat tgctgtgcag tggtaacccg
     1621 caaatgtcat aaagtgccgc actgtctgcc tcgctaagcg ttgccagata ctcttcaccg
     1681 cgacggtcat accatgccag cattggcgtc aggatctcgc catgttcgtc aagaaacacc
     1741 cctgactcgc caaaactggc aatgcttatc cgcctgaccg gcagcggcga agcggcgttc
     1801 agttgcgcta tcgcctggcg gacctcctgc cagagggcgt cgatatcgaa atcgacatcg
     1861 ccctgtgggg agatctgctt tgccgtcacg aatttatgcg cgcccaacgt cgttgcgtcc
     1921 aggcaggaaa agcaggtgac tttgcaattg gtggtgccaa tatcgataac gatagctgcg
     1981 ctgttatcag gcataaaccg tgctccgcgt cattcggaaa atgccataca gtaccagggc
     2041 gataaccggc accagaccaa tggcgatgat attgccgttg aagatttcca gcagccagaa
     2101 gcggaacggg ttaccgccat caaggaaact ggaaacctgg ccgctggagc cttcggcaaa
     2161 tttgaagtta acgtctttcg ccatgccggt aataaacggt gcgattttag tcgcaatcca
     2221 caggtcggca ataatcaccg ggatcgccgc cagaacggtg cggaaaatat tgccacggct
     2281 ggctaaagca atcatcgacg ccattaccgc caggttggca aggtcaccaa gcggcaggac
     2341 ttcattacct gggaggacaa acgcgataaa gaccgagatt ggcgtcagca gcagggcggt
     2401 ggagataatc gccgggttac ctacggcaac ggcgatatcc aggccgatat agagatcgtc
     2461 acggtcaggg tatttggcat tgagatactt tttaatggct tcagacagcg gtaataaacc
     2521 ttccatcagg atgcgtacca tgcgtggcag gatgaacatc acaccgccaa tgctgatgcc
     2581 aagcagcaag acttttttga aatcgtatcc ggcaattacg ccgagcagaa tgcccagaat
     2641 agtgccaacc atcatcggtt cgccgaagat gccaaacttt ttctgtacgg tttccgggtc
     2701 gatatgaata cgattgaggc cagggatatg gtcgatgatt ttgtcgacca acagaccgac
     2761 cggaaagaac accaccgaag agagcgtcgg caaagagatc ccttccaggc caaagtattt
     2821 ttgtaccagt ggcgcagacc agtcggcgag tttaagtact accaccgcag caatcgccgc
     2881 tgccagcaaa ccaaagaaca ggctgccggt gctgtaataa accagcgtac cggcaagggc
     2941 aaaatgccag tagttccaca tatctacgtc aacggtgcgg gttttattta acgccagcat
     3001 cgccacgtta atcagaatag tcaggggaat aacaaacggg gcaattggcg aaccccatgt
     3061 aatagcggaa agcggcggcc agcctaaatc caccaccggt aaattaattc cggtgcgttc
     3121 aaccatcgcc tgggccgctg gcccgacatt gctggtaaga atatcgaaaa tggcataaat
     3181 accgacaaaa ccaatgccga ccgttaatga cgagcgtaag gctttagcgg gttttacttt
     3241 aaaacagaga gcaagaataa ataacaccaa cggcaacatt accgtggggc caaatcccaa
     3301 tatatattgc acaatattat agagggtatg cgcgatatcg ttcataataa acctcttttt
     3361 caggcgagag cgcacctcgc gttagcgcct gatggagtgg gttggaatta attattaatt
     3421 actttttaag aatcgaaaca atttgcgcca gtactttctc ttcgccaata ccggtaataa
     3481 tcggcaagcc gttaactacc gggacgtcga gttcataagg cactttagtc gttgagacta
     3541 ccagtgctac gccatcagta tttccaggta aagaggcaac gttggtttgg gaataattga
     3601 cgttaagacc gtgctctttg caatactcca tgactttttc tgttaccgcc gtggatgtgg
     3661 caatacctgt ggcgcagaca aataaaatgg tttgactcat atttcacctc gttcattcat
     3721 tttgtttaag attactgtgt gtatttctgt tgtttccatc gtaagcaagg cttgcataaa
     3781 gggcgtatcc tgaatcatct ccataatcca gcccagggca tttaattgtt tattactttc
     3841 acttaaggcg agcaaaaaaa tcaccctgac aggtacgggg tcaggatcgc cgcccatatc
     3901 ggcaaactct accggttccg gcaggatgcc gacggcaatt gcattgtgcc ggacatgttt
     3961 atggtccgtg tgcggaatgg cgacgcacgg tggttcggta ggcagccccg ttgggtacgt
     4021 tgcctcacgt tttagcacgt gatcggcaaa atcagggacc accagctcag cagcaaccaa
     4081 cggttgcgct aactgacgga tgatctgttc aacgcttaac cctgccggaa gtcgttgaat
     4141 ataattttcc tgaaactgta tgccttgcat tttattcctc ataaaaaagt gtatttaact
     4201 cacttcccgc cgttgcagca cggtgcccag ataatattta tttcctttta accagacgtt
     4261 gccaaactca acgggtaact ctcgggagaa aaagactaac tgctccagat gcaaaacggg
     4321 tgcatcttca ctgatatcta aaaaatgacc gcgttcatta ccaattaatc gcgcggcata
     4381 gcgactttcc gagtaacgaa tttttctttg cgacaaactt tctattgttg gaaataaatt
     4441 gtgttgatta aaatcgattt ccacgatgcc ggggcatagc tcaatattga tacggttctc
     4501 tatcagcatc gctttttcat caccaattga acgtaaacgc tcaaggtaaa gaatatcctg
     4561 accgggcgtt attcttaatt tctctgccac ataacgatta gccggttcaa tccgtgacgt
     4621 aataacttca gtggtaaaat gtattttctg gctttccagc gattccgcga atgacaataa
     4681 tccttcacct aacggatatg ccacgttctc ttttttgaca aaagttccct tcccctggac
     4741 ctgaatcaac acgccttctt ttaccagttg ggcgaccgct tttttaacgg ttccccggct
     4801 cacttccagc atatccataa tctggtattc cgaaggaatg cgatcgtctt ccaccagatc
     4861 cccggtataa atactttctc ttatccactc caccacttgt tgatagagcg gtatttgtga
     4921 cgacttactc aatgatttca tggcgcgcac ctgaacattt acagttgtct atggacaatc
     4981 atggacaact tcaagcgtat agtaggtgaa caggcgaaaa agaggatcga agcgtctcac
     5041 attttagggg accaaatcgt gatcaaaaaa atgtgacgtg agtcgatatt tggcagaagt
     5101 ctactgcgcc gtcaggaaat aacggcgcgt tgggaggtgt taatcttcct tcggcaggca
     5161 ctgcaaatgc ccgtgccgca cgccgcgctg ggcgatgacg tcatcggcaa aatgctgcac
     5221 gtcgcccatg tcacctttca acacggcgat ttccagacag tcgtcgtgat taatgtgcac
     5281 atgcagagta gcaaccgaga gatcgtggtg atgatgctgg gtggagacaa tacggctggc
     5341 taagtcgcgt ttttcgtgtt catacacata cgacagcacc gcgaaacctt gtgtgccgtg
     5401 ttgttgagtg gcttcctgcg ccagggcgct acgcagaata tcgcggatcg cctcggaacg
     5461 gttgttataa ccacgacgct ggctcaggct gtccagcgtc tccagtaaat cgtcatcaag
     5521 cgtgatggtg actcgttgca tttgcgttaa accttttctg tggtgcgacg gcgcacgggg
     5581 aatgcgggta ataccgcgtt ttgtagcaca cgtccggcgt cagaggaaaa ggttaattta
     5641 tctcccacca cctgggtttc gacgatttgt ccgttgtcca tcaccatcac ccgctgacag
     5701 aagcgttcca ccaggcgtaa gtcgtgggtg atgaacaggc aggcggtgcc aaactgttgt
     5761 tgcagctttt tcagcaggcg aatgacgccc gcttgtaaca tgagatcaag gttcgaaacc
     5821 gcttcatcca gaatcagcag tttcggttcg accgccagcg cgcgtgccag gcagacacgc
     5881 tggagctggc cgccgcttaa ctgcggtggg cgtttgtcga gcacgctgtc gtcgagatcg
     5941 accgccttca gcatttcact ggcgcgcgcc agttgttcgg cttttttcag cgatagcagg
     6001 tggcgcatcg gttcacgcag gatctcgcgc acggttttgc gcggattcac ggcgctgatg
     6061 gagtcctgaa acaccatttg aatatcacgg cggaacgctt tacgttgcgc gcgattgagc
     6121 ttcgccaacg gttcgccgcg ccagctaata tttccctgtg agggcgactc taaccctacc
     6181 agcagccgcg cgagggtact ttttccgcag ccgctgcgcc ccagcagggc gacggtttca
     6241 ccgcttttca gggccaggga aacgttattc agcaccgcct ggtgttgatg tttgctgcta
     6301 aatccaccgt gcgcgtagtg atgggaaagg tcggagacgt taagtaaagt catgatgcca
     6361 gctccatacc gtagagggcg agatgagcgg aaaccaggct gcgcgttacc gcatgtttgg
     6421 gggcgttaaa cagcgtttct acatcgccct gttcaacaat cttaccgtga gacattaccg
     6481 ccacatcgtc tgccagacgc gctaccacgc ccatatcatg ggtaaccagc agcattcccg
     6541 gtgcttgttt ttgcataatg ctttccagca gatcgaggat gcgcgcctgt gctaccacgt
     6601 cgagatcggt ggtcggttca tcggcgatga taaatggtga ttcgcacagc accgccatcg
     6661 caatcatcat gcgctgcaac atgccgccgc tcatctcgaa cgggtagagc ttcagcacgc
     6721 gcgcggcgtt ttccagcccc accgcttcta tggcagcggt aagcgtagcg tcatcggcgg
     6781 gtttccccag cgccaggcag gtttcacgcg cgtgggtgtg catggtgtgc agcggattaa
     6841 aggcactgcg cgggttctgc atgatggtgg caattttgat tccgcgcagg gcgcagggag
     6901 aaaccggttt tccatcggcc aaaatttccc ccgccgtctg gcgaacgcca gcaggcagaa
     6961 tgcccagcgt cgcggcgcag gtcagcgact tcccgctgcc actaccgccg actaacgcca
     7021 gcacacgccc acgttgcagg gttaacgata cgccgtgcac cagcggctgc gcggcctgta
     7081 gcgcgatatt acggagttca atctgttgtg gcattagtgt gcgtgctccg tcaccagatg
     7141 agggtccaga tgatcgcgca gtgcgtcacc caccaggtta aaggccatca cgctgataaa
     7201 cagcgccagc cccggccaga acatttgcag cggctgggtc cagatatact gacgtgcatc
     7261 gttaatcatc acgccccatt cggcggtcgg cgcggtcacg ccgaggccga ggaaggacat
     7321 ccccgcgacg tgtagcatca tatgaccgat atccagcgtc gccagcacca gcagcgaggg
     7381 gatcaccgca cctgccagat gatcaacaaa cacccgcaca tggcccgcgc cagaaagccg
     7441 tgatgccagc acaaactcgc gctggcgcag tgaaatcacc agactgcgca ccatgcgcgc
     7501 gtaccatgcc cagtgcgaaa gggcgatagc gataattacg ttggtcaacc cggtgccaag
     7561 cacgccgacc ataaagaacg acagaatcga ggttgggaag gtcataaaca tatccgcgac
     7621 gcgcatggtg gcctgatcaa cgcgcccgcc aatcagcccg gcgctgccgc caataatcag
     7681 ccctaatgtc agcaccaaca gcaggcaggc cattaccgag ccgagcgaca tgcgggtcgc
     7741 tgccatcagc cgcgagaaaa tatcgcgccc taaatgatcg gtgcccagcc agtgctgcgc
     7801 atccggcgag agcaggcgcg acggcaaatc aatagcctga gggtcatacg gcagccacca
     7861 ctggctggtg agcgcaatca gcgccagcag ggcgataatg atcagcgcca ggcgaaccga
     7921 ccagcgggag gagaggaaaa agttcacgcg tgcgctcctt catgacgacg aatacgcggg
     7981 tccagcgcgg cgttgagcaa atcgacaatc aaattacaga ccacaaaaac caccaccatc
     8041 atcagcgtaa agcactggat caccggatag tcacggttaa aaatcgccga caccgcatag
     8101 cgcccgacgc ccggccaggc aaagatgttt tcgataatca tcgtcccgcc aatcagttcg
     8161 ccaatgtgca tccccacggc agtgatcatc ggcagcgagg cattgcgcag aatatggcgg
     8221 cgttcggtct gtttgtcgct cagaccacgc agtcgtgccc aggtgacgtg acgctgaccg
     8281 gcgacgtcca gcatactggc gcgcagcaaa cgcgcgttaa tcgccagcga cataaaggcg
     8341 atggataccg caggcaaaat gatgtgctgc cagccgccgt aacccatcgc gggtagccat
     8401 tgcagataca ccgaaaacgc catcaccagt aaaaacgcca gccagaagtt aggcatcgac
     8461 acgccgagaa acgcgatgaa acggacggcg aaatccggca gacgatcgcg atggcgcgct
     8521 gcccagatac cgagcggtac ggaggtgagc agaattaata ccagcgccgc acctgcaagt
     8581 tccagcgtgg cgggcaggaa attcagcata tcgtccagca ccgggcgttg gctggcgaat
     8641 gagataccaa agtcaagatg cagcgccttc cacaaccagg tgccgtactg gacatacagc
     8701 ggctgatcca gccccagcat ggtgcgggta gaggccagca tctccggcgt cggcggcagg
     8761 ttagataaac gcaaataatc aagcgccggg tcgccggtgc cgaggcgcag catcaaaaaa
     8821 atgatcaccg aggcggcgag caccatcggg atcagcagca gaaagcggcg taatacataa
     8881 cgcaacatta aggtttcacc ggtttaatct gttcgaacgg gatttcggtg gcgatcggcg
     8941 cgtaggggat gttacccagt tccggttttg aaaccaccat cattgagatg taactgatag
     9001 gcagataaac cgcctcgtca tgcagacggg tcagaatgtc gcgatacagc gcctgacgtt
     9061 gcgtttcgtc atgggtcgcc agcacttcgc cgatctcttt atcaatcagc ggcttgtcgg
     9121 ctaaaccttg ctgtgcctgg aagtcagcgt gcgacggtac gcgcattgaa ctcaggaagg
     9181 cgtgtggatc gtatggcgcg ccccaggtgc ggtggaaaat catgccaaaa cgaccgtcgc
     9241 gctgacgagc atagatactg ctctcttctt cgccaatcag cgagacatct gcgccaatct
     9301 ggcgcatatc agcctgaatg atttccgcca tcgatttgct taacgcatcg gtgccgatga
     9361 acgacagttc aatgcgcagc ggctgaccat ttttctcgcg gatgtctttg cctgcaggca
     9421 gcgtccaacc ggctttttcc agcaacgctt tcgctttctg cggatcgtac tggcgcggtt
     9481 tcaggccgag gttggcgtag ggcacagaag gggcaaacag ggtgtcggcg acctgctggg
     9541 taccatacaa cgcgttatca atcagcgatt ttttgttcac cgcgtaatta agagcttcac
     9601 gtactgccag ctcgttggtg ggggctttgg cggtattgag cgccagcatc acggtttcga
     9661 tcggctgtga cagttgagtg tggtaagccg ggttctggct aaagcgggcg aaggtatcga
     9721 gcggtaataa cccttcgttt ccgtacaaca ggtcgatatc gccagtttca aacgccaccg
     9781 cgcgggtagt cgggtccggg atcacgttaa aggtgatctt tttaatcgcc ggcttttcgc
     9841 cccagtagtt ttcgttacgg acgaagacat cgtactgatt cagtttcgat tcctgtaaaa
     9901 cccacggccc ggtgccaatc ggcgctttaa ttccgttcat ggtttcgtgg tttttaaact
     9961 gcgagggtgc gataaaacgg aaaggacggg gcagggccag ttcttgcagg aaaggatagt
    10021 aggcgctttt cagggtgatt tgcagctcgt ttttgccgag tgctttgaca tcaacaatct
    10081 ggtttgccag ctccagccag gcgtgacgtt gacggttatc gagcactgca cggaagtttt
    10141 ctgccgccgc ctctgcatca aacggttcgc cgttggagaa cttcacgtca tcacgcaggg
    10201 tgaaggtcca ggttttacca tcttctgaat gagtccagct ttttgccagc cacgggatca
    10261 ccgaaccgtc tgcctgatat ttcaccagcg gttcataaac catgctctgg gcgaacattt
    10321 ggttaggcgt gtaaaggtgc gggtttagtg gcccgacatt caccggccag gcggtggtga
    10381 tttcatctgg tgcagcggca tggacgataa aagacgcaca agccagcagc gcaaatagag
    10441 tgcggcggag tgtagagaac atggtaaccc caatggatta aaatagatgg cggaaataag
    10501 tatgacgatt ttaagtattc gtcatactga ttacctgttc tggatcaata gatgggcagt
    10561 cgggtgggcg gatcagttaa ctgaatcgat ccattgcaca ctgtcggcgg tgagcgtaaa
    10621 gggggtaggg gtgcagatcg ccaggcttaa attttctagc tgacaatggc tgactgacag
    10681 cgaggagtta taggtgctgt ctacgctgac gatttgccag gcgctgccgc cacgctgttt
    10741 aacgatggct tctttgcgcg tccagatgcg ccagaacatt tccagttgct gatcaggatg
    10801 cacggcgtcc atctcggcat gttccccaag gctgaatacg gcgttcgcca gccagcgcca
    10861 gttggcgcgc gggcgaatca cttcgatatc gcagccgact tcgccttcat cgctcaacag
    10921 cagggcgata tcgtcaccac tatggcttaa gttgaaccat agcggcgttt ccggcgcgaa
    10981 tgcaggtttg ccttgttcgc catagatgat ctccggtagc ggggaaagcg tgtgcgaaag
    11041 caatgcacgc cccgccagcc agcgttcacg tcgtggacct tgcggtgctt gctcgcgtaa
    11101 acccggtggc agaggagctg cgcttaaggt cgaaactttc cccagaacta tccgatacat
    11161 atcagggcca acgtttaatg gaaaatgaaa gtgcgtatcg taacacttgt cgcctcatcc
    11221 cggtaaccga cttttcggtc tgcctggccc cagtaaaatc gccagtttgc tgccgccttt
    11281 ggtggtttcc atccagattt tacacacgct ggttaatggc accgaaagta gcatccctac
    11341 cgggccgagc agccatcccc aaattaacaa tgaaagaaat accaccatgg tggacatccc
    11401 caggcgatgg cccatcatcc gtggttctaa aatattgccg atgaccatat ggaccactaa
    11461 aaacaatgcg ccgaccagaa tacattcgta aataccatta aacagcagca cctgaatcat
    11521 tggcggtacg gcggaaatta ccgcaccgat attgggcacg tagttgagca aaaacgccag
    11581 tactgcccac atcagcgcaa actgcacccc catcagctcc agccccagcc agacgattat
    11641 gcctgtccat aaactgagta gcgtctttaa cgccagataa tgcgaaacgc ctttaagtgc
    11701 gcggtgtagt ccggcgatgt gaatctgtgg attattcaac gcgaaacgca ttttgtaagg
    11761 gacgtggcgc acttcaaaca gcataaaaac tacggtcatc accagcaaaa gcacgctcgc
    11821 cattgccccg gaaagcccgg tcattagcgc cgtggtgaag gtaaccactt tttccgagtc
    11881 catccgctgt agtattcgct ccggcgacat atgcaaatta agaaaaggca acatctcctg
    11941 caatttaaaa agtttgcgcg tcagctcctt attaaacctc ggcagcatag agataaattc
    12001 gttaaacgat gccgccagta cgccgaccag cgcggtgagt gcgatcagca tcaccacaac
    12061 cacaatcgta atggcaacgg ggcgttgtac cccccgacga ataaaccagg tgacgagcgg
    12121 gttgagaacg atggcaaaaa acagcgccag taatagctgc acaatgatat ctgccgctgc
    12181 gtgaatgccc gcgaggatca ctaccagcga ggccaacttg agcagaatgt gcatgcccgt
    12241 tttatcgggt tgaggggttt ccattggggc ttccttgtga ctttttgtat taagtgtagc
    12301 gggagtcacg ccagcaatat tctgattctt actgctgatt ttcacgtcag cacatggtaa
    12361 aaatgaaaca ctgttgtaaa aatgtggtga tcctcatgcc cgaacccgta gccgaacccg
    12421 cgctaaacgg attgcgcctg aatttgcgca ttgtctccat tgtcatgttt aacttcgcca
    12481 gctaccttac catcgggttg ccgctcgctg tattaccggg ctatgtccat gatgtaatgg
    12541 gatttagtgc attctgggca ggggtggtta tcagcctgca atattttgcc accttgctga
    12601 gccgtcctca tgccggacgt tacgccgatt tactgggacc caaaaagatt gtcgtcttcg
    12661 gtttatgcgg ctgctttttg agcggtttgg gatatctgac ggcaggatta accgccagtc
    12721 tgcccgtcat cagcctgtta ttactgtgcc tggggcgcgt aatccttggg attggacaaa
    12781 gttttgccgg aacgggatcg accctgtggg gtgttggcgt ggttggttcg ctacatatcg
    12841 ggcgggtgat ttcgtggaac ggtattgtca cttacggggc gatggcgatg ggtgcgccgt
    12901 taggtgtcgt gttttatcac tggggcggat tgcaggcgtt agcgttaatc attatgggcg
    12961 tggcgctggt ggccattttg ttggcgatcc cgcgtccgac ggtaaaagcc agtaaaggca
    13021 aaccgctgcc gtttcgtgcg gtgcttgggc gcgtctggct gtacggtatg gcgctggcac
    13081 tggcttccgc cggatttggc gttatcgcca cctttatcac gctgttttat gacgctaaag
    13141 gttgggacgg tgcggctttc gcgctgacgc tgtttagctg tgcgtttgtc ggtacgcgtt
    13201 tgttattccc taacggcatt aaccgtatcg gcggcttaaa cgtggcgatg atttgcttta
    13261 gcgttgagat aatcggcctg ctactggttg gcgtggcgac tatgccgtgg atggcgaaaa
    13321 tcggcgtctt actggcgggg gcggggtttt cgctggtgtt ccctgcattg ggcgtagtgg
    13381 cggtaaaagc ggttccgcag caaaatcagg gggcggcgct ggcaacttac accgtattta
    13441 tggatttatc gcttggcgtg accggaccac tggctgggct ggtgatgagt tgggcgggcg
    13501 ttccggtgat ttatctggcg gcggcgggac tggttgcaat cgcgttatta ctgacgtggc
    13561 gattaaaaaa acggcccccg gtggaaatac ctgaggccgc ctcatcatct taaaacttac
    13621 tgaataacca gcgtattaat gatgttttct gcggtggtct gtgctttttg ctgattgtca
    13681 gcgggcagcg taatttgcat ggttagcagt tgattaccca cgttacccag aataacggaa
    13741 gagtacgccg tctggccttt cgcggagata atgctgtcta actgctgcat tttgtgacct
    13801 ttcagctcaa tggctttatt ggttaccact tgcagctgcg gatcgcggct acgttgctga
    13861 tcttccaggc gcttcgccag caccgccaga tcttctttcg gatcatcgcc catgatgacg
    13921 atgactgctt tctgcccggt ggcgtcggac cagacatgca tgttattggc ctgcgttccc
    13981 agcttaccgc tctggtcggt catatccgct ggcagcgaga aacttaactt gccatcaagc
    14041 aggttgacgg gattcccggt cgcgttactt tctgcgaccg aaccctgcgc cgtagcgtta
    14101 gtgtctttat catcacaggc cgcaagcccc ataaccagca ggccaattcc gacatattta
    14161 accagattgc gcattgactt cttcctttcg ataaacggcc ataacggctc attcatccat
    14221 cttatcacaa ctctgataac gaacctttaa ctcgcctgca aagcgttgat ttcggattta
    14281 tctgccagtc ttttcaacag catattgagt aatacgccat acattggcag gaagaaaacg
    14341 atactgatta acactttgaa acagtaatcg actagcgcga tttccatcca gtgttcagcc
    14401 ataaaggcat ccgggctacg ccagaaggca atgaagaaga aggccagcgt gtcgctgacg
    14461 ttaccgaaca gtgtggacgc tgtcggtgcc agccaccagt gacgactctg acgcaggcgg
    14521 ttaaaaacgt gcacgtcgag gatctgcccc agcgcgtagg ccatgaaact ggcggtggcg
    14581 atacgggcga caaacaggtt gaagtgggcg agcgcgccga atccttgcca tgaccccata
    14641 taaaacagcg atgagatgac gtaggagatc agcaatgcgg ggatcattac cgcaaagatg
    14701 atgcgacgag ccagcggcgc gccaaaaata cgcacggtca ggtcagtagc gagaaagata
    14761 aacggaaagc taaacgcgcc ccaggtggta tgaaaaccaa aaatggagac cggaagctga
    14821 accagatagt tactggaggt gatcaccagc agatgaaata acgataacca gaacaacgcc
    14881 ttatagcgtt gcgtttgcga gaaaacgttc atattgtacc tttttgatta accattgggg
    14941 tgagggaacc caatacgtac gacacgtctg ttaccttaac ataataacgt gctgaccctt
    15001 ttttcgagcc gccgcatgat actgctttac gttgacaatg caatggttaa ttttcacgca
    15061 atcgttaacc tggtttgctt acggactcac agggcgtaaa ctagcgccgt ttttttatgt
    15121 gatgagaaga aaatgaccga tctcttttcc agccctgacc acacactcga cgcacttggc
    15181 ctgcgctgcc cagaaccagt aatgatggtg cgcaaaaccg tgcgcaatat gcagcctggc
    15241 gaaacgttgt tgattattgc cgacgatccg gcgacgaccc gcgatattcc tgggttttgt
    15301 acctttatgg aacacgaact ggttgcgaaa gagacggatg gactgcctta tcgttatttg
    15361 attcgtaaag gcggttgatg ggggaagatg ggcttcgata ctgccttttg ccctcaccct
    15421 aaccctctcc ccagagaggc gaggggaccg atcgcactca atgttgctgc ttgaaaccgg
    15481 tttgtaggcc ggataaggcg ttcaagctgc atctggcgat acggtgcaca atgcctgatg
    15541 cgacgctgtc gcgtcttatc atgcctacga gttcgatgct ctgtgtaggc cggataaggt
    15601 gttcacgccg catccggcat tgttggaccg tagactgata gcctctttat ttcctgcgca
    15661 ataaccgtaa cgcattcgcc gtcaccagca ccgtcgcacc cgtatctgcc agcactgcca
    15721 gccatagtcc ggtcatccct aacagcgtgg tgacgaggaa gatccctttc agccccagcg
    15781 caatggtgat gttctggcgg atattggcgt gagtggcgcg tgccagttca atcatttgca
    15841 ccaggccgcg caggtggtta tgggttaatg ctgcgtcggc ggtttccagg gccacgtctg
    15901 tgccactgcc cattgcaatc ccaatagcgg cggctttcat cgctggcgcg tcgttaatac
    15961 cgtcgccgac catcgccagc ggcgcatgtt gattcagctc ggtcaccgct ttaactttat
    16021 cttccggcaa gaggcccgct ttaaattcca gccccagttc cccggcgatt gccgctgctg
    16081 cgcgtggatt atcgcctgta aggatcaccc ctttgacgcc cagcgcgttc agttcactga
    16141 tagcagttgc agcatcggcg cgcagggtat cctgcaacgc aatgacaccc agcacgtcat
    16201 cattacatac taccagcact accgtttgcc cggcgctttc cagttcatta atcaaaccag
    16261 caaatgcatc agcaggatgt tttcctgcag cgcatatcaa cacgcgctca ccgttaacct
    16321 gcgcttcaat gccagacccg accagcgccc gctgtgattg ggcggtggga atggcgagtt
    16381 cagcaacctg tgcttcgcgc acaatggcct gtgccagtgg atgcgtcgcg ccttgctcga
    16441 ccgccgccgc cagtgtcagc agttcagatt cactaatacc cgttgccgga tgaatcgcgg
    16501 taacgcgcgg tttaccgacg gtcagcgtac cggttttatc aaacgccacc tgggtaacac
    16561 gacccagctg ctccagcgcc gcgccgcctt taatcaacgc cccacgacgc gctgctgccg
    16621 ccaacccgga ggtaatcgcc gcgggcgtgg agataactaa agcacacggg caaccaatca
    16681 gcagcagcgt cagcccttta taaatccact cctgccagct ggcggcaaac agcagcggcg
    16741 gcaccagcgt taccagcagg gcgacggcca taatcgctgg cgtgtaaata cggctgaaac
    16801 ggtcgataaa ccgctcaata ggtgcgcggc gctcttcggc ttcttcaatc agtttcagaa
    16861 tccggtcaat ggcgctggca cccggttctg acagcacttc cagcgttacc agacggtcta
    16921 cgctggtggt gcctgcagga actttatcgc ccgtcgcgcg ctccacagga atggattctc
    16981 cggtcagggc actttcatca aaactggcaa acggtgagag cagtttaccg tcggcaggca
    17041 aacgcccacc tgccgcaact tcaatcacat cgccaggccg caggctgttt attgccacct
    17101 cttcacgctc accgttacgc agacgcgtgg cggtttctgg cttcagcgcc attaacgcgc
    17161 tgaccccttg acgcgcgcga ctggcggccc agccttccag tcgttcacca atcaaaaaca
    17221 gcagcaacac catcgcagct tcagccgttg cgccaataaa cagcgcacca atagcggcta
    17281 cgctcattaa ggtttcaatg gcgaagtagc tgccagattt gatcagccgt aatgcctgac
    17341 gagcaattgg gtacagccca accagcgtgg tcgcgataaa cgccagttga ccaaacggat
    17401 gattgaactg ctccagcccc caactgattg ccatcatcac gataagcgta atcagcggca
    17461 gattctcttt caggcgtgat tcttgtggtt cgtcggaggc ctgttcatcg cgcagggaat
    17521 agcccgcttt ttgcaccgca gattcaactt gtgcccggat gtcattgtcg gcatccacca
    17581 ccagtttttc ggtggcgaac aacacctgta cctgattcac gcctgcaagc tggcgcacgg
    17641 cattttccac tttgcgcgca caggcggcgc agtccatacc gctgactttc cagctatagc
    17701 gggtgccgga gacgttttca gagagagttg gcgtgctgga acatgcgccg ccgcagcaac
    17761 agtcgttggt gttctgtacc gtagttagcg gtttgaacgc agcaaattga ggggctttct
    17821 tgccgtgatt gtcaggagtc gacatggcat cctccggtta agttttttct cattaaccga
    17881 aggatacact ctggagtcga ctccagagtc aagttttatc agagatacag cgagcggacg
    17941 atgaggaagt gaccagcaaa gtagcaggcc gcggcaatgg cgttatccgc acggaagcgt
    18001 cggcgatagt gactacccag ccagacaaag ttactgataa acagcaacga agcgccgaca
    18061 aacgcagaga gcgccggagc ggtcggacgg aagaaccaca gttcacctgc cagccacacc
    18121 atcaccagcg tcatgccgat aaacgtgcag ataggccaac gatactcttc cagacgcgtc
    18181 cagataatcg ccagtaacag cgcgcccaga accagcagca ccaacggcag aggccagaag
    18241 aaagagagcg tcatctgact ggcgaaatag atggtgtaca gcaggtgcga gaggaaaaac
    18301 gcgccgatgg cgtacatcag acgttgacgt ggcaacaggg ttagcgcatc gcccagcagt
    18361 gaggcacaca gccccgccag caccagatag ctaatggcgt cgaacatcgg cgcttgccag
    18421 gccagcaaca gcaggagaag aagggttaac ggtttaaaca cccagcgttg ccaggttggc
    18481 ccacgatacg atgcatccac agatagccat gcggaaagac agacagcgat aaacgaccaa
    18541 agcatcttag ttccttgaat tcgttatttt ttcgtaagcg gaatgcctac ggtcttgctt
    18601 ccagtttagt ggcgagggtg gacggatgac aacaccataa ctggcagggt atgcttattt
    18661 ccgcattttc cgatgaggga taaagatgag taaaccacct cttttcttta ttgttatcat
    18721 tggcttaatc atcgtctccg catcgtttcg ttttatgcaa caacgacggg aaaaagctga
    18781 taatgatatg gcaccgctcc agcaaaagct ggtggtggtg agcaacaagc gggaaaaacc
    18841 gattaacgat cgccgttcgc gccagcagga agtgaccccg gcaggcacca gtatgcgcta
    18901 tgaggcaagc ttcaaaccgc aaagcggagg aatggagcag acgtttcgcc tcgacgccca
    18961 gcagtaccac gccctgacag tgggtgataa aggtacgctg agctataaag gaacgcgctt
    19021 tgtcagcttt gtaggcgaac aataaggtta ttttttaact ttaaatttct tctgccagac
    19081 cagcagttca aacacgccga aaaggaaaat ccgcaccttt tcggcggtgg tcatctgcgg
    19141 gccatctttc ggtaaagtgg atttcaatag cgccagctgc ataccatgca tcagcacggt
    19201 aaaaatcagc gccacgttaa cgaaaatatt cagcgggcgt gggaagggat gcaccaggtt
    19261 gaggattaaa aatccccaaa cacagagcat taacaaacga ccaatattaa tcagcatcac
    19321 tttctccttg tgcttcgcgt tgatacagcc gataagccac ctgacccgcc actttttccc
    19381 gatgtaatga ccagtttgcc ggaacagtgg gcagaccgtt ttcgacttcg ctttcgacat
    19441 aaatcagggc ttcgtcagcc agccagccgt tatcttccag taaatttatc gtctcttcta
    19501 acaagccacg gcggaacggt ggatcgacaa acacgatatt atgcggtgtg cctttttgcg
    19561 ccaggaatga catcgcgttg ctgttcacca cgcgtgcatt gcctgctttt agtgtcgcca
    19621 gattcttaat taactgctga gaaaccgcac gatccatctc aatcaacgtg gcccccgcag
    19681 cgtagcgcga taacgcttcc agccccagcg cgccgctccc ggcgaagcaa tccagacatt
    19741 gggcgtcaac aatgaccgga gccagccagt taaacaacgt ttcgcgtacg cggtcggtgg
    19801 tggggcgcag acctggacta tcaggaaccg ggagtttacg gcctcgccac tgcccgccaa
    19861 taatgcggat ttggccgctg ccggaatgat tcggtttttt catgataaat tgctcaatcc
    19921 gccagataac acatacttgc aggcggtgat gtgaattaag ttaagtgata gactatttca
    19981 tcattttttt agctgctatg tacatagcgt taacgctgtg ccatgaagca acagcgagga
    20041 gtgtagtcgc aaatggcgaa agaaaaaaaa cgtggctttt tttcctggct gggctttggt
    20101 caaaaagagc agatcccgga aaaagagaca gaagttcaga atgaacaacc agttgtagaa
    20161 gaaatcgttc aggcgcaaga gcctgtgaag gcttctgaac acgccgttga agagcagccg
    20221 caggcgcata ctgaagccga ggcggaaact tttgctgccg acgttgtgga agttactgaa
    20281 caggttgctg aaagtgaaaa agcgcagcct gaagcggaag tcgttgcaca gccggaaccg
    20341 gtcgtagaag aaacgccgga gccagtggct atcgaacgtg aagaactgcc gttgccggaa
    20401 gacgtcaacg ccgaagcggt ttcgccagaa gagtggcagg ccgaagcgga aaccgtagag
    20461 attgtcgaag cggcggaaga agaagcggct aaagaagaaa tcaccgacga agagccggag
    20521 gcacaggcgc tggctgccga agcggcagaa gaggcggtaa tggtggtttc tccggcagaa
    20581 gaagagcagc cggtggaaga aatcgctcag gagcaggaaa aaccgaccaa agaaggtttc
    20641 ttcgcgcgcc tgaaacgcag cctgttaaaa accaaagaaa atctcggttc cggatttatc
    20701 agtctgttcc gcggtaaaaa aatcgacgat gatctgtttg aagagctgga agaacagctg
    20761 ttgatcgccg atgtgggcgt ggaaaccaca cgtaaaatta tcaccaatct gacggaaggc
    20821 gcatcccgca agcagcttcg tgatgccgag gcgctctatg gcctgctgaa agaagagatg
    20881 ggcgagattc tggcgaaagt cgatgagccg ctgaatgttg aaggtaaaac gccgttcgtg
    20941 atcctgatgg tgggcgtcaa cggtgtgggt aaaaccacga cgattggtaa gctggcgcgt
    21001 cagtttgagc agcagggtaa atcggtgatg ctggcggcag gcgatacttt ccgtgcggca
    21061 gcggttgaac agcttcaggt ctggggtcag cgcaacaata ttccggtgat tgcccagcat
    21121 accggtgctg attccgcttc tgttatcttc gacgccattc aggccgctaa agcgcgtaac
    21181 atcgacgtcc tgattgccga taccgccgga cgcctgcaaa acaaatcgca cctgatggaa
    21241 gagttgaaga aaatcgtccg cgtgatgaag aaactcgacg ttgaagctcc gcatgaagtt
    21301 atgctgacta ttgatgccag caccgggcag aatgcggtaa gccaggccaa actgttccac
    21361 gaagccgttg gcttaaccgg catcacgcta acgaaactgg acggcacggc gaaaggcggg
    21421 gtaattttct cggtggctga ccagtttggt atccctatcc gctacattgg tgtcggcgaa
    21481 cgtattgagg atttgcgtcc gtttaaggcg gacgacttta tagaggcact ttttgcccga
    21541 gaggattaac aatgattcgc tttgaacatg tcagcaaggc ttatctcggt gggagacagg
    21601 cgctgcaggg cgttacgttc catatgcagc cgggtgagat ggcgtttctg accggtcatt
    21661 ccggcgcagg gaaaagtacc ctcctgaagc tgatctgtgg gattgagcgg cccagcgccg
    21721 ggaaaatctg gtttagcggc catgacatca cgcgtctgaa aaaccgtgaa gttccgtttc
    21781 tgcgccgcca gattggcatg attttccagg atcaccatct actgatggac cgtactgtct
    21841 atgataacgt ggcgatcccg ctgattatcg ccggtgccag cggtgatgac attcgtcgcc
    21901 gggtgtcggc ggcgctggat aaagtcgggc tactggacaa agcgaagaac ttccctattc
    21961 agctttccgg cggtgaacaa cagcgtgttg gcattgcccg cgcggtggtg aacaagcccg
    22021 cggtactgct ggcggacgaa ccgactggta acctggacga cgcgctgtcg gaaggcattt
    22081 tacgtctgtt tgaagagttt aaccgcgttg gagtaaccgt attgatggca acgcacgaca
    22141 tcaacctgat ttcgcgacgt tcctatcgca tgctcaccct gagcgatggt cacttgcatg
    22201 gaggcgtggg ccatgaataa gcgcgatgca atcaatcaca ttcggcagtt tggcgggcgt
    22261 ctggatcgct tccgtaaatc ggtcggcggc tctggcgacg gcggtcgtaa cgcgccgaaa
    22321 cgcgcgaaat cctcgccaaa accggtaaat cgcaaaacca acgttttcaa cgaacaggta
    22381 cgctatgcct tccacggcgc attgcaggat ctgaaaagca aaccgttcgc cacgttttta
    22441 acggtgatgg ttatcgccat ctccctgacg ctgcctagcg tctgttatat ggtgtacaaa
    22501 aacgttaacc aggcggcgac gcaatattat ccgtcaccgc aaatcactgt ttatctgcaa
    22561 aaaacgctgg atgatgacgc cgctgcgggc gtggtggcac agttgcaggc cgagcaaggc
    22621 gtggagaaag tgaattatct ttctcgtgaa gacgcactgg gcgagtaccg taactggtct
    22681 ggttttggtg gtgcgctgga tatgctggaa gaaaacccgc ttccggcagt agcggtggtg
    22741 atcccgaaac tcgattttca ggggacggag tcactgaata cgctgcgtga tcgtatcacg
    22801 cagattaacg gcattgacga agtgcggatg gatgacagct ggtttgcccg cctggcggcg
    22861 ttaaccgggc tggtcgggcg cgtttccgcg atgatcggcg tgttgatggt ggcagcagtg
    22921 tttctcgtca tcggcaacag tgtgcgtctg agcatctttg ctcgccgtga ctctattaac
    22981 gtgcagaaac tgattggtgc gacggatggt ttcatcctgc gcccgttcct gtacggtggc
    23041 gcactgctgg gattttctgg cgcattgttg tcattaattt tgtcagaaat tctggtgctg
    23101 cgattgtcat cggcggttgc ggaagtggca caggttttcg gaacgaagtt tgatatcaat
    23161 ggtttatcgt tcgatgaatg cctgctattg ctgctggtat gctcgatgat tggctgggtg
    23221 gcagcgtggc ttgccacggt acaacattta cgccacttta cgcctgaata ataaaagcgt
    23281 gttatactct ttccctgcaa tgggttccgt agcagggaaa gagaccccgt tgtctcttcc
    23341 cggtatttca tctctatgtc acattttgtg cgtaatttat tcacagcgtt gcattgaact
    23401 tgtggataaa atcacggtct gataaaacag tgaatgataa cctcgttgct cttaagctct
    23461 ggcacagttg ttgctaccac tgaagcgcca gaagatatcg attgagagga tttgaatgac
    23521 tgacaaaatg caaagtttag ctttagcccc agttggcaac ctggattcct acatccgggc
    23581 agctaacgcg tggccgatgt tgtcggctga cgaggagcgg gcgctggctg aaaagctgca
    23641 ttaccatggc gatctggaag cagctaaaac gctgatcctg tctcacctgc ggtttgttgt
    23701 tcatattgct cgtaattatg cgggctatgg cctgccacag gcggatttga ttcaggaagg
    23761 taacatcggc ctgatgaaag cagtgcgccg ttttaacccg gaagtgggtg tgcgcctggt
    23821 ctccttcgcc gttcactgga tcaaagcaga gatccacgaa tatgtcctgc gtaactggcg
    23881 tatcgtcaaa gttgcgacca ccaaagcgca acgcaaactg ttcttcaacc tgcgtaaaac
    23941 taagcagcgt ctgggctggt ttaaccagga tgaagtcgaa atggtggccc gtgaactggg
    24001 cgtaaccagc aaagacgtac gtgagatgga atcgcgtatg gcggcacagg acatgacctt
    24061 tgacctgtct tccgacgacg attccgacag ccagccgatg gctccggtgc tctatctgca
    24121 ggataaatca tctaactttg ccgacggcat cgaagatgat aactgggaag agcaggcggc
    24181 aaaccgtctg accgacgcga tgcaaggtct ggacgagcgt agccaggata tcatccgcgc
    24241 gcgctggctg gacgaagaca acaagtccac gttgcaggag ctggctgacc gttacggcgt
    24301 ttccgctgag cgtgtgcgtc agctggaaaa gaacgcgatg aaaaaattgc gtgctgccat
    24361 tgaagcgtaa tttccgctat taagcagaga accttggatg agagtccggg gtttttgttt
    24421 tttgggcctc tgtaataatc aatttcccct ccggcaaaac gccaatcccc actcagattg
    24481 ttaataaact gtcaaaatag ctattccaat atcataaaaa tcgggtatgt tttagcagag
    24541 tatgctgcta aagcacgggt agtcatgcat aaaacgaaat aaagtgctga acaacaacat
    24601 cacaacacac gtaataacca gaagaatggg gattctcagg atgaacatga agggtaaagc
    24661 gttactggca ggatgtatcg cgctggcatt cagcaatatg gctctggcag aagatattaa
    24721 agtcgcggtc gtgggcgcaa tgtccggtcc ggtggcccag tacggtgacc aggagttcac
    24781 tggcgcagag caggcagtag cggatatcaa tgctaaaggt ggtatcaaag gcaacaaact
    24841 gcaaatcgta aaatatgacg atgcctgtga cccgaaacag gcggttgcgg tggcgaacaa
    24901 agtcgttaac gacggcatta aatacgtgat tggtcacctc tgctcttcat caacgcagcc
    24961 agcgtctgac atctacgaag acgaaggcat tttaatgatc accccagcgg caaccgcacc
    25021 agagctgacc gcccgtggct atcagctgat cctgcgcacc accggcctgg actccgacca
    25081 ggggccgacg gcggcgaaat atatacttga gaaagtgaaa ccgcaacgca ttgctatcgt
    25141 tcacgacaaa cagcagtacg gcgaaggtct ggcgcgtgca gtgcaggacg gcctgaagaa
    25201 aggcaatgct aacgtggtgt tctttgatgg catcaccgcc ggggaaaaag atttctctac
    25261 cctggtggcg cgtttgaaga aagagaatat cgatttcgtt tactacggcg gttatcaccc
    25321 ggaaatgggg caaatcctgc gtcaggcacg cgcggcaggg ctgaaaactc agtttatggg
    25381 gccagaaggc gtggctaacg tctcgctgtc taacattgcg ggcgaatcag cggaagggct
    25441 gctggtgacc aagccgaaga actacgatca ggttccggcg aacaaaccca ttgttgacgc
    25501 gatcaaagcg aaaaaacagg acccaagcgg cgcgttcgtc tggaccacct acgccgcgct
    25561 gcaatctttg caagcggggc tgaatcagtc tgacgatccg gcggagatcg ctaaatacct
    25621 gaaagcgaac tcggttgata cggtgatggg gccgctgacc tgggatgaga aaggcgatct
    25681 gaaaggcttt gagttcggtg tgtttgactg gcacgccaac ggcacggcga ctgatgcgaa
    25741 gtaatcatta atcggcaact ttgggttgcc gccaaattgc taatatcgag tacgttgctt
    25801 catgccggat gcggcgtaaa cgccttatcc ggcctacaag attcaaagaa atcagtaaat
    25861 tgcagcacac attgtaggcc tgataagcgt agcgcatcag gcaatatcac ttttgaaatc
    25921 aaacttgacg attaatgctt ctcccagcca ccctgttgtg ccgtaaatcc cagtgctcgc
    25981 ataaacgccg tcatcacgcc gcgatcttcc acgccagcat ccgccatcca ccaacatgaa
    26041 acgtcaggat tgttacgcaa aacctcttcc agcagatatt gccccacacc gcgacgtcgg
    26101 gtgacttccc gcacgcgtag agaatccagt gccccctcgg tgccgcttaa ggttacccgc
    26161 acggcagcga gcaggcgctc gttaaaacgc gcggcgtaga tacggtagtt atcgtcaacc
    26221 tgtaaagagg aaggggaata ttccggccag atcttttgca ggtcaatccg gtcctggtcg
    26281 ctaaagtttt ctaatcgaat gatggtcagc ttcatgggga acccgtgtaa atcacaaaag
    26341 tggagccagt gtagcgaaat aatttaatcg gaggctttct cttttttatt tcttttggca
    26401 ggtgattaat tttttttcag caataattac aaagttaaaa cattaga
//
LOCUS       DAZFFE010000063        26430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFFE010000063 DAZFFE010000000
VERSION     DAZFFE010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.063"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            10..1476
                     /gene="gspA"
                     /locus_tag="ACHJ69_002806"
     CDS             10..1476
                     /gene="gspA"
                     /locus_tag="ACHJ69_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFO5015757.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDRAWTRDEMAGQLLALHRTDSRCPLII
                     IENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEHE
                     LKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFTA
                     KQWRMIYRVLGDKRPRKMQLAVVMSGTILALTCGWLLLSSFTAPLPVPAWLIPVTPVV
                     KQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLVCKSGNASLQTLV
                     DQNLPWIASLKVGDKKLPVVVVRVGDATVDVLVGQQTWTLTHKWFELVWTGDYLLLWK
                     MSPEGESTITRDSSEEEILWLETMLNRALHISTESSAEWRPLLVEKIKQFQKSHHLKT
                     DGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1478..1897
                     /gene="gspB"
                     /locus_tag="ACHJ69_002807"
     CDS             1478..1897
                     /gene="gspB"
                     /locus_tag="ACHJ69_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HFO5015758.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVDAETPAFKQSTQHYFFKKQPLPVVESVEEEDEPGVA
                     VENAPSSSEDEENTVEESDEKAGLRERVKNALNELER"
     gene            2135..2446
                     /gene="rpsJ"
                     /locus_tag="ACHJ69_002808"
     CDS             2135..2446
                     /gene="rpsJ"
                     /locus_tag="ACHJ69_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO5015759.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2479..3108
                     /gene="rplC"
                     /locus_tag="ACHJ69_002809"
     CDS             2479..3108
                     /gene="rplC"
                     /locus_tag="ACHJ69_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO5015760.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3119..3724
                     /gene="rplD"
                     /locus_tag="ACHJ69_002810"
     CDS             3119..3724
                     /gene="rplD"
                     /locus_tag="ACHJ69_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO5015761.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3721..4023
                     /gene="rplW"
                     /locus_tag="ACHJ69_002811"
     CDS             3721..4023
                     /gene="rplW"
                     /locus_tag="ACHJ69_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO5015762.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4041..4862
                     /gene="rplB"
                     /locus_tag="ACHJ69_002812"
     CDS             4041..4862
                     /gene="rplB"
                     /locus_tag="ACHJ69_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO5015763.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4879..5157
                     /gene="rpsS"
                     /locus_tag="ACHJ69_002813"
     CDS             4879..5157
                     /gene="rpsS"
                     /locus_tag="ACHJ69_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO5015764.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5172..5504
                     /gene="rplV"
                     /locus_tag="ACHJ69_002814"
     CDS             5172..5504
                     /gene="rplV"
                     /locus_tag="ACHJ69_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO5015765.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5522..6223
                     /gene="rpsC"
                     /locus_tag="ACHJ69_002815"
     CDS             5522..6223
                     /gene="rpsC"
                     /locus_tag="ACHJ69_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO5015766.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6236..6646
                     /gene="rplP"
                     /locus_tag="ACHJ69_002816"
     CDS             6236..6646
                     /gene="rplP"
                     /locus_tag="ACHJ69_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO5015767.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6646..6837
                     /gene="rpmC"
                     /locus_tag="ACHJ69_002817"
     CDS             6646..6837
                     /gene="rpmC"
                     /locus_tag="ACHJ69_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO5015768.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6837..7091
                     /gene="rpsQ"
                     /locus_tag="ACHJ69_002818"
     CDS             6837..7091
                     /gene="rpsQ"
                     /locus_tag="ACHJ69_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO5015769.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7256..7627
                     /gene="rplN"
                     /locus_tag="ACHJ69_002819"
     CDS             7256..7627
                     /gene="rplN"
                     /locus_tag="ACHJ69_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO5015770.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7638..7952
                     /gene="rplX"
                     /locus_tag="ACHJ69_002820"
     CDS             7638..7952
                     /gene="rplX"
                     /locus_tag="ACHJ69_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO5015771.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSFGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNATTGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7967..8506
                     /gene="rplE"
                     /locus_tag="ACHJ69_002821"
     CDS             7967..8506
                     /gene="rplE"
                     /locus_tag="ACHJ69_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO5015772.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8521..8826
                     /gene="rpsN"
                     /locus_tag="ACHJ69_002822"
     CDS             8521..8826
                     /gene="rpsN"
                     /locus_tag="ACHJ69_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO5015773.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8860..9252
                     /gene="rpsH"
                     /locus_tag="ACHJ69_002823"
     CDS             8860..9252
                     /gene="rpsH"
                     /locus_tag="ACHJ69_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO5015774.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9265..9798
                     /gene="rplF"
                     /locus_tag="ACHJ69_002824"
     CDS             9265..9798
                     /gene="rplF"
                     /locus_tag="ACHJ69_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO5015775.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9808..10161
                     /gene="rplR"
                     /locus_tag="ACHJ69_002825"
     CDS             9808..10161
                     /gene="rplR"
                     /locus_tag="ACHJ69_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO5015776.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10176..10679
                     /gene="rpsE"
                     /locus_tag="ACHJ69_002826"
     CDS             10176..10679
                     /gene="rpsE"
                     /locus_tag="ACHJ69_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO5015777.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10683..10862
                     /gene="rpmD"
                     /locus_tag="ACHJ69_002827"
     CDS             10683..10862
                     /gene="rpmD"
                     /locus_tag="ACHJ69_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO5015778.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10866..11300
                     /gene="rplO"
                     /locus_tag="ACHJ69_002828"
     CDS             10866..11300
                     /gene="rplO"
                     /locus_tag="ACHJ69_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO5015779.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11308..12639
                     /gene="secY"
                     /locus_tag="ACHJ69_002829"
     CDS             11308..12639
                     /gene="secY"
                     /locus_tag="ACHJ69_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO5015780.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12671..12787
                     /gene="rpmJ"
                     /locus_tag="ACHJ69_002830"
     CDS             12671..12787
                     /gene="rpmJ"
                     /locus_tag="ACHJ69_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO5015781.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12934..13290
                     /gene="rpsM"
                     /locus_tag="ACHJ69_002831"
     CDS             12934..13290
                     /gene="rpsM"
                     /locus_tag="ACHJ69_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO5015782.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13307..13696
                     /gene="rpsK"
                     /locus_tag="ACHJ69_002832"
     CDS             13307..13696
                     /gene="rpsK"
                     /locus_tag="ACHJ69_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO5015783.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13730..14350
                     /gene="rpsD"
                     /locus_tag="ACHJ69_002833"
     CDS             13730..14350
                     /gene="rpsD"
                     /locus_tag="ACHJ69_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO5015784.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14376..15365
                     /gene="rpoA"
                     /locus_tag="ACHJ69_002834"
     CDS             14376..15365
                     /gene="rpoA"
                     /locus_tag="ACHJ69_002834"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO5015785.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15406..15789
                     /gene="rplQ"
                     /locus_tag="ACHJ69_002835"
     CDS             15406..15789
                     /gene="rplQ"
                     /locus_tag="ACHJ69_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO5015786.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15896..16264
                     /gene="yhdN"
                     /locus_tag="ACHJ69_002836"
     CDS             15896..16264
                     /gene="yhdN"
                     /locus_tag="ACHJ69_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO5015787.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLVGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELENRREAIQLLDILKGIRHDDPQYQEVSRRLSLLQLKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16275..16700
                     /gene="zntR"
                     /locus_tag="ACHJ69_002837"
     CDS             16275..16700
                     /gene="zntR"
                     /locus_tag="ACHJ69_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO5015788.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTKSD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDTCCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16756..16974
                     /gene="arfA"
                     /locus_tag="ACHJ69_002838"
     CDS             16756..16974
                     /gene="arfA"
                     /locus_tag="ACHJ69_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO5015789.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSDAC"
     gene            complement(16971..17384)
                     /gene="mscL"
                     /locus_tag="ACHJ69_002839"
     CDS             complement(16971..17384)
                     /gene="mscL"
                     /locus_tag="ACHJ69_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO5015790.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLREAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAATPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17514..18890)
                     /gene="trkA"
                     /locus_tag="ACHJ69_002840"
     CDS             complement(17514..18890)
                     /gene="trkA"
                     /locus_tag="ACHJ69_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO5015791.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQIAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18912..20201)
                     /gene="rsmB"
                     /locus_tag="ACHJ69_002841"
     CDS             complement(18912..20201)
                     /gene="rsmB"
                     /locus_tag="ACHJ69_002841"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO5015792.1"
                     /translation="MKKQRNLRSMAAQAIEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQDELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVQLETPAPVH
                     ALPGFEEGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVLAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWSHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20253..21200)
                     /gene="fmt"
                     /locus_tag="ACHJ69_002842"
     CDS             complement(20253..21200)
                     /gene="fmt"
                     /locus_tag="ACHJ69_002842"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO5015793.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNIVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEDKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTTTKAAPGTILEANKQGIQVA
                     TGDGILNLLSMQPAGKKAMSVQDLLNSRREWFVPGNRLA"
     gene            complement(21215..21724)
                     /gene="def"
                     /locus_tag="ACHJ69_002843"
     CDS             complement(21215..21724)
                     /gene="def"
                     /locus_tag="ACHJ69_002843"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO5015794.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21854..22978
                     /gene="dprA"
                     /locus_tag="ACHJ69_002844"
     CDS             21854..22978
                     /gene="dprA"
                     /locus_tag="ACHJ69_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO5015795.1"
                     /translation="MVDTEIWLRLISISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22950..23423
                     /gene="smg"
                     /locus_tag="ACHJ69_002845"
     CDS             22950..23423
                     /gene="smg"
                     /locus_tag="ACHJ69_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO5015796.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23452..23994
                     /gene="yrdD"
                     /locus_tag="ACHJ69_002846"
     CDS             23452..23994
                     /gene="yrdD"
                     /locus_tag="ACHJ69_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO5015797.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPISAE"
     gene            23999..24571
                     /gene="tsaC"
                     /locus_tag="ACHJ69_002847"
     CDS             23999..24571
                     /gene="tsaC"
                     /locus_tag="ACHJ69_002847"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO5015798.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24576..25394
                     /gene="aroE"
                     /locus_tag="ACHJ69_002848"
     CDS             24576..25394
                     /gene="aroE"
                     /locus_tag="ACHJ69_002848"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO5015799.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVAITNRTVSRAEELTK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            25391..25648
                     /gene="yrdB"
                     /locus_tag="ACHJ69_002849"
     CDS             25391..25648
                     /gene="yrdB"
                     /locus_tag="ACHJ69_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO5015800.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25624..26178)
                     /gene="yrdA"
                     /locus_tag="ACHJ69_002850"
     CDS             complement(25624..26178)
                     /gene="yrdA"
                     /locus_tag="ACHJ69_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO5015801.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            complement(26222..>26430)
                     /locus_tag="ACHJ69_002851"
     CDS             complement(26222..>26430)
                     /locus_tag="ACHJ69_002851"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015802.1"
                     /translation="QDFFSLLLRPSCEVFHVVVSTEAHYRDPNFLHKYFFDLFFCLLL
                     FHPFCCIRTQNGAFLHAKRLAQGQ"
BASE COUNT         6750 a         6225 c         6897 g         6558 t
ORIGIN      
        1 ggaagttcta tgtctacgag aagagaagtc attctttcct ggttgcgtga gaaacgtcag
       61 acctggcgtc tgtgctattt gttgggtgag gctggaagtg gaaaaacctg gctggcgcag
      121 caactacaaa aagataaaca tcgccgtgtg attactttaa gcctcgttgt ttcctggcaa
      181 ggtaaggccg catggatcgt taccgacgat aacgcggctg aacagggctg ccgtgacaga
      241 gcctggacgc gagatgagat ggcgggtcaa ttactggcac ttcatcgaac tgatagtcgc
      301 tgccctctta ttattattga aaatgctcac ctgaatcacc gccgcatact tgatgatctc
      361 caacgcgcga tatctcttat tcctgacggt caatttttgc tgatcggcag acccgatcga
      421 aaagttgaac gtgattttaa aaaacagggc attgaacttg tctcgacagg tcgtctgacg
      481 gagcacgagc ttaaggcaag catccttgaa ggtcagaata ttgaccagcc cgatctcctt
      541 ttaaccgcca gagttctgaa acggatagct ttattatgtc ggggagatcg cagaaagctg
      601 gcgcttgccg gcgaaacaat tcgcttatta cagcaagccg agcagaccag cgttttcact
      661 gcaaaacaat ggcgaatgat ttaccgcgta ttaggtgata agcgcccccg gaaaatgcag
      721 cttgccgtgg tgatgagtgg aaccattctc gcacttacct gtggttggct actgctgtcc
      781 tctttcacag ctccgttacc cgttcctgcg tggctgatac ccgtaacgcc agtagttaaa
      841 caagatatga ctaaggatat tgctcatgtg gtgatgcgcg atagtgaggc gctaagcgtg
      901 ttatacggcg tatgggggta cgaggttcca gccgattcgg cctggtgcga tcaggcagta
      961 agagcggggc tggtatgtaa aagtgggaac gcttctttac aaacattggt agatcagaat
     1021 ttgccctgga tcgcatcgct taaagtcggg gacaaaaaat tgcctgttgt ggtcgttcgc
     1081 gtcggtgatg ctaccgttga tgtactcgta gggcagcaaa cctggacgct gacacataaa
     1141 tggtttgagt tggtatggac gggtgattat cttctgttgt ggaaaatgtc gccggaaggg
     1201 gagagtacca ttacgcgtga tagcagcgag gaagagattc tctggctgga aacgatgtta
     1261 aaccgcgcat tgcatatttc gacagaatct tcggcagagt ggcgtccctt gttggtagaa
     1321 aaaataaagc aatttcaaaa aagccatcac ttgaaaactg acggtgttgt tggcttttca
     1381 acgttagtac atctctggca ggtggcgggg gagagtgcct acttatatcg ggatgaagca
     1441 aatatttccc cagaaacaac agtgaagggg aaatgacatg tttgaattct atatcgccgc
     1501 gcgtgagcag aaagaaacgg gtcaccctgg tatcttctcc cgacaaaaac acagcaccat
     1561 aatatatgtc atttgtttat tgctgatttg cctgtggttt gcaggaatgg tgttggttgg
     1621 tgggtatgcc cggcagctat gggcactttg gatagtaaaa gcagaagtca ctgtagatgc
     1681 tgaaacgccc gcttttaaac aatcaacaca acactatttt ttcaaaaaac agccactccc
     1741 cgtcgtcgaa tccgtcgagg aggaagatga accaggcgtc gcggttgaga atgccccctc
     1801 ttcttctgaa gacgaagaaa atactgtaga agaaagcgac gagaaagccg gtttaagaga
     1861 gcgtgtcaaa aatgcgctga acgaacttga gagataaccc aaaggctgtt tactttacta
     1921 gcaatacgct tgcgttcggt ggttaagtat gtataatgcg cgggcttgtc gtagttgaca
     1981 gcaggttcaa tctgaacctc agttagccga atttggctac ctaacaatgc tcccaatcgg
     2041 ggagctacgt aagaacggtt acactctccc atcaatcgta atgggtctga ggagtaatca
     2101 ttttcgttta taaaataatt ggagctctgg tctcatgcag aaccaaagaa tccgtatccg
     2161 cctgaaagcg tttgatcatc gtctgatcga tcaagcaacc gcggaaatcg tcgagactgc
     2221 taagcgcact ggtgcgcagg tccgtggtcc gatcccgctg ccgacacgca aagagcgctt
     2281 cactgttctg atctccccgc acgtcaacaa agacgcgcgc gatcagtacg aaatccgtac
     2341 tcacttgcgt ctggttgaca tcgttgagcc aaccgagaaa accgttgatg ctctgatgcg
     2401 tctggatctg gctgccggtg tagacgtgca gatcagcctg ggttaatcag gtcattgagc
     2461 gattgagagg ttgaaacaat gattggttta gtcggtaaaa aagtgggtat gacccgtatc
     2521 ttcacagaag acggcgtttc tatcccagta accgtaatcg aagttgaagc aaaccgcgtt
     2581 actcaggtta aagacctggc taacgatggc taccgtgcta ttcaggtgac caccggtgct
     2641 aaaaaagcta accgtgtgac caagcctgaa gctggacact tcgctaaagc tggcgtagaa
     2701 gctggccgtg gtctgtggga attccgcctg gctgaaggcg aagagttcac tgtaggtcag
     2761 agcattagcg ttgaactgtt tgctgacgtt aaaaaagttg acgtaactgg cacctctaaa
     2821 ggtaaaggtt tcgcaggtac cgttaagcgc tggaacttcc gtacccagga cgctactcac
     2881 ggtaactcct tgtctcaccg cgttccgggt tctatcggtc agaaccagac tccgggcaaa
     2941 gtgttcaaag gcaagaaaat ggcaggtcag atgggtaacg aacgtgtaac cgttcagagc
     3001 cttgacgtag tacgcgttga cgctgagcgc aacctgctgc tggttaaagg cgctgtcccg
     3061 ggtgcaaccg gtagcgacct gatcgttaaa ccagctgtga aggcgtaagg agatagcaat
     3121 ggaattggta ttgaaagacg cgcagagcgc gctgactgtt tccgaaacta ccttcggtcg
     3181 tgatttcaac gaagcgctgg ttcaccaggt tgttgttgct tatgcagctg gtgctcgtca
     3241 gggtactcgt gctcagaaga ctcgtgctga agtaactggt tccggtaaaa aaccgtggcg
     3301 ccagaaaggc accggccgtg cgcgttctgg ttctatcaag agcccgatct ggcgttctgg
     3361 tggcgtgacc tttgctgctc gtccgcagga ccacagtcaa aaagttaaca agaagatgta
     3421 ccgcggcgcg ctgaaaagca tcctgtccga actggtacgt caggatcgtc tgatcgttgt
     3481 cgagaagttc tctgtagaag cgccgaaaac taagctgctg gcacagaaac tgaaagacat
     3541 ggctctggaa gatgtgctga tcatcaccgg tgagctggac gagaacctgt tcctggctgc
     3601 gcgcaacctg cacaaggttg acgtacgcga tgcaactggt atcgacccgg ttagcctgat
     3661 cgccttcgac aaagtcgtaa tgactgctga tgctgttaag caagttgagg agatgctggc
     3721 atgattcgtg aagaacgtct gctgaaggtg ctgcgtgcac cgcacgtttc tgaaaaagcg
     3781 tctactgcga tggaaaaatc caacaccatc gtactcaaag ttgctaaaga cgcgaccaaa
     3841 gcagaaatca aagctgctgt gcagaaactg tttgaagtcg aagtcgaagt cgttaacacc
     3901 ctggtagtta aagggaaagt taaacgtcac ggacagcgta tcggtcgtcg tagcgactgg
     3961 aaaaaagctt acgtcaccct gaaagaaggc cagaatctgg acttcgttgg cggcgctgag
     4021 taagtcggag gagtaataca atggcagttg ttaaatgtaa accgacatct ccgggtcgtc
     4081 gccacgtagt taaagtggtt aaccctgagc tgcacaaggg caaacctttt gctccgttgc
     4141 tggaaaaaaa cagcaaatcc ggtggtcgta acaacaatgg ccgtatcacc actcgtcata
     4201 tcggtggtgg ccacaagcag gcttaccgta ttgttgactt caaacgcaac aaagacggta
     4261 tcccggcagt tgttgaacgt cttgagtacg atccgaaccg ttccgcgaac atcgcgctgg
     4321 ttctgtacaa agacggtgaa cgccgttaca tcctggcccc taaaggcctg aaagctggcg
     4381 accagattca gtctggcgtt gatgctgcaa tcaaaccagg taacaccctg ccgatgcgca
     4441 acatcccggt tggttctact gttcataacg tagaaatgaa accaggtaaa ggcggtcagc
     4501 tggcacgttc cgctggtact tacgttcaga tcgttgctcg tgatggtgct tatgtcaccc
     4561 tgcgtctgcg ttctggtgaa atgcgtaaag tagaagcaga ctgccgtgca actctgggcg
     4621 aagttggcaa tgctgagcat atgctgcgcg ttctgggtaa agcaggtgct gcacgctggc
     4681 gtggtgttcg tccgaccgtt cgcggtaccg cgatgaaccc ggtagaccac ccacatggtg
     4741 gtggtgaagg tcgtaacttt ggtaagcacc cggtaactcc gtggggcgtt cagaccaaag
     4801 gtaagaagac ccgcagcaac aagcgtactg ataaattcat cgtacgtcgc cgtagcaaat
     4861 aattttagag gataagccat gccacgttct ctcaagaaag gtccttttat tgacctgcac
     4921 ttgctgaaga aggtagagaa agcggtggaa agcggagaca agaagcccct gcgcacttgg
     4981 tcccgtcgtt caacgatctt tcctaacatg atcggtttga ccatcgctgt ccataatggt
     5041 cgtcagcacg ttccggtatt tgtaaccgac gaaatggtcg gtcacaaact gggtgaattc
     5101 gcaccgactc gtacttatcg cggccacgct gctgataaaa aagcgaagaa gaaataaggt
     5161 aggaggaaga gatggaaact atcgctaaac atcgccatgc tcgttcttct gctcagaagg
     5221 ttcgccttgt tgctgacctg attcgcggta agaaagtgtc gcaggctctg gatattttga
     5281 cctacaccaa caagaaagcg gctgtactgg tcaagaaagt tctggaatct gccattgcta
     5341 acgctgaaca caacgatggc gctgacattg acgatctgaa agttacgaaa attttcgtag
     5401 acgaaggccc gagcatgaag cgcattatgc cgcgtgcaaa aggtcgtgca gatcgcatcc
     5461 tgaagcgcac cagccacatc actgtggttg tgtccgatcg ctgagactct ggagactagc
     5521 aatgggtcag aaagtacatc ctaatggtat tcgcctgggt attgtaaaac catggaactc
     5581 tacctggttt gcgaacacca aagaattcgc tgacaacctg gacagcgatt ttaaagtacg
     5641 tcagtacctg actaaggaac tggctaaagc gtccgtatct cgtatcgtta tcgagcgtcc
     5701 ggctaagagc atccgtgtaa ccattcacac tgctcgcccg ggtatcgtta tcggtaaaaa
     5761 aggtgaagac gtagaaaaac tgcgtaaggt cgtagcggac atcgctggcg ttcctgcaca
     5821 gatcaacatc gccgaagttc gtaagcctga actggacgca aaactggttg ctgacagcat
     5881 cacttctcag ctggaacgtc gcgttatgtt ccgtcgtgct atgaagcgtg ctgtacagaa
     5941 cgcaatgcgt ctgggcgcta aaggtattaa agttgaagtt agcggccgtc tgggcggcgc
     6001 ggaaatcgca cgtaccgaat ggtaccgcga aggtcgcgta ccgctgcaca ctctgcgtgc
     6061 tgacatcgac tacaacacct ctgaagcgca caccacttac ggtgtaatcg gcgttaaagt
     6121 gtggatcttc aaaggcgaga tcctgggtgg tatggctgct gttgaacaac cggaaaaacc
     6181 ggctgctcag cctaaaaagc agcagcgtaa aggccgtaaa taaggagcgt cgctgatgtt
     6241 acaaccaaag cgtacaaaat tccgtaaaat gcacaaaggc cgtaaccgcg gtctggcgca
     6301 gggtacggat gttagcttcg gcagcttcgg tctgaaagct gttggccgtg gtcgtctgac
     6361 tgcccgtcag atcgaagcag cacgtcgtgc tatgacccgt gcagttaagc gtcaaggtaa
     6421 gatctggatc cgtgtgttcc cggacaaacc gatcactgaa aagccgctgg cagtgcgtat
     6481 gggtaaaggt aaaggtaacg tggagtattg ggttgccttg attcagccgg gtaaagtcct
     6541 gtatgaaatg gacggtgttc cggaagagct ggcccgtgaa gcattcaagc tggcagcagc
     6601 gaaactgccg attaaaacca cctttgtaac taagacggtg atgtaatgaa agcaaaagag
     6661 ctgcgtgaga agagcgttga agagctgaac accgagctgc tgaacctgct gcgtgagcag
     6721 ttcaacctgc gtatgcaggc tgcaagtggc cagctgcaac agtctcacct gttgaagcaa
     6781 gtgcgtcgcg atgtcgcacg cgttaagact ttactgaacg agaaggcggg tgcgtaatga
     6841 ccgataaaat ccgtactctg caaggtcgcg ttgttagcga caaaatggag aaatccattg
     6901 ttgttgctat cgaacgtttt gtgaaacacc cgatctacgg taaattcatc aagcgtacga
     6961 ccaaactgca cgtacatgac gagaacaacg aatgcggtat cggtgacgtg gttgaaatcc
     7021 gcgaatgccg tccgctgtcc aagactaaat cctggacgct ggttcgcgtt gtagagaaag
     7081 cggttctgta atacagtaca ctctctcgat acgaataaac ggctcagaaa tgagccgttt
     7141 attttttcta cccatatcct tgaagcggtg ttataatgcc gcgccctcga tatggggatt
     7201 tttaacgacc tgattttcgg gtctcagtag tagttgacat tagcggagca ctaaaatgat
     7261 ccaagaacag actatgctga acgtcgccga caactccggt gcacgtcgcg taatgtgtat
     7321 caaggttctg ggtggctcgc accgtcgcta cgcaggcgta ggcgacatca tcaagatcac
     7381 catcaaagaa gcaattccgc gtggtaaggt caaaaaaggt gatgtgctga aggcggtagt
     7441 ggtgcgcacc aagaagggtg ttcgtcgccc ggacggttct gtcattcgct tcgatggtaa
     7501 tgcttgtgtt cttctgaaca acaacagcga gcagcctatc ggtacgcgta tttttgggcc
     7561 ggtaactcgt gagcttcgta gtgagaagtt catgaaaatt atctctctgg caccagaagt
     7621 actctaagga gcgaatcatg gcagcgaaaa tccgtcgtga tgacgaagtt atcgtgttaa
     7681 ccggtaaaga taaaggtaaa cgcggtaaag ttaagaatgt cctgtctttc ggcaaggtca
     7741 ttgttgaagg tatcaacctg gttaagaaac atcagaagcc ggttccggcc ctgaaccaac
     7801 cgggtggcat cgttgaaaaa gaagccgcta ttcaggtttc caacgtagca atcttcaatg
     7861 cgacaaccgg caaggctgac cgtgtaggct ttagattcga agacggtaaa aaagtccgtt
     7921 tcttcaagtc taacagcgaa actatcaagt aatttggagt agtacgatgg cgaaactgca
     7981 tgattactac aaagacgaag tagttaaaaa actcatgact gagtttaact acaattctgt
     8041 catgcaagtc cctcgggtcg agaagatcac cctgaacatg ggtgttggtg aagcgatcgc
     8101 tgacaaaaaa ctgctggata acgcagcagc agacctggca gcaatctccg gtcaaaaacc
     8161 gctgatcacc aaagcacgca aatctgttgc aggcttcaaa atccgtcagg gctatccgat
     8221 cggctgtaaa gtaactctgc gtggcgaacg catgtgggag ttctttgagc gcctgatcac
     8281 tattgctgta cctcgtatcc gtgacttccg tggcctgtcc gctaagtctt tcgacggtcg
     8341 tggtaactac agcatgggtg tccgtgagca gatcatcttc ccagaaatcg actacgataa
     8401 agtcgaccgc gttcgtggtt tggatattac cattaccact actgcgaaat ctgacgaaga
     8461 aggccgcgct ctgctggctg cctttgactt cccgttccgc aagtaaggta gggttactaa
     8521 atggctaagc aatcaatgaa agcacgcgaa gtaaaacgcg tagctttagc tgataaatac
     8581 ttcgcgaaac gcgctgaact gaaagcgatc atctctgatg tgaacgcttc cgacgaagat
     8641 cgttggaacg ctgttctcaa gctgcagact ctgccgcgtg attccagccc gtctcgtcag
     8701 cgtaaccgct gccgtcaaac aggtcgtccg catggtttcc tgcggaagtt cgggttgagc
     8761 cgtattaagg tccgtgaagc cgctatgcgc ggtgaaatcc cgggtctgaa aaaggctagc
     8821 tggtaattgt caccaattga atcacgggag taaagacaga tgagcatgca agatccgatc
     8881 gcggatatgc tgacccgtat ccgtaacggt caggccgcga acaaagctgc ggtcaccatg
     8941 ccttcctcca agctgaaagt ggcaatcgcc aacgtgctga aggaagaagg ttttattgaa
     9001 gattttaaag ttgaaggcga caccaagcct gaactggaac ttactctgaa gtatttccag
     9061 ggcaaagctg ttgtagaaag cattcagcgt gtcagccgcc caggtctgcg catctataaa
     9121 cgtaaagatg agctgccgaa agttatggcg ggtctgggta tcgcagttgt ttctacctct
     9181 aaaggtgtta tgactgatcg tgcagcgcgc caggctggtc ttggtggcga aattatctgc
     9241 tacgtagcct aatcggagga aaaaatgtct cgtgttgcta aagcaccggt cgttgttcct
     9301 gccggcgttg acgtaaaaat caacggtcag gttattacga tcaaaggtaa aaacggcgag
     9361 ctgactcgta ctctcaacga tgctgttgaa gttaaacatg cagataatac cctgaccttc
     9421 ggtccgcgtg atggttacgc agacggttgg gcacaggctg gtaccgcgcg tgccctgctg
     9481 aactcaatgg ttatcggtgt taccgaaggc ttcactaaga agctgcagct ggttggtgta
     9541 ggttaccgtg cagcggttaa aggcaatgtg attaacctgt ctctgggttt ctctcatcct
     9601 gttgaccatc agctgcctgc gggtatcact gctgaatgtc cgactcagac tgaaatcgtg
     9661 ctgaaaggcg ctgataagca ggtgatcggc caggttgcag cggatctgcg cgcctaccgt
     9721 cgtcctgagc cttacaaagg caagggtgtt cgttacgccg acgaagtcgt gcgtaccaaa
     9781 gaggctaaga agaagtaagg taacactatg gataagaaat ctgctcgtat ccgtcgtgcg
     9841 acccgcgcac gccgcaagct ccaggagctg ggcgcaactc gcctggtggt acatcgtacc
     9901 ccgcgtcaca tttacgcaca ggtaattgca ccgaacggtt ctgaagttct ggtagctgct
     9961 tctactgtag aaaaagctat cgctgaacaa ctgaagtaca ccggtaacaa agacgcggct
    10021 gcagctgtgg gtaaagctgt cgctgaacgc gctctggaaa aaggcatcaa agatgtatcc
    10081 tttgaccgtt ccgggttcca atatcatggt cgtgtccagg cactggcaga tgctgcccgt
    10141 gaagctggcc ttcagttcta aggtagaggt gtaagatggc tcacatcgaa aaacaagctg
    10201 gcgaactgca ggaaaagctg atcgcggtaa accgcgtatc taaaaccgtt aaaggtggtc
    10261 gtattttctc cttcacagct ctgactgtag ttggcgatgg taacggtcgc gttggttttg
    10321 gttacggtaa agcgcgtgaa gttccagcag cgatccagaa agcgatggaa aaagcccgtc
    10381 gcaatatgat taacgtcgcg ctgaataacg gcactctgca acaccctgtt aaaggtgttc
    10441 acacgggttc tcgcgtattc atgcagccgg cttccgaagg taccggtatc atcgccggtg
    10501 gtgcaatgcg tgccgttctg gaagtcgctg gggttcataa cgttctggct aaagcgtatg
    10561 gttccaccaa cccgattaac gtggttcgtg caactatcga tggcctggaa aatatgaatt
    10621 ctccagaaat ggtcgctgcc aagcgtggta aatccgttga agaaattctg gggaaataaa
    10681 ccatggcaaa gactattaaa attactcaaa cccgcagtgc aatcggtcgt ctgccgaaac
    10741 acaaggcaac gctgcttggc ctgggtctgc gtcgtattgg tcacaccgta gagcgcgagg
    10801 atactcctgc tattcgcggt atgatcaacg cggtttcctt catggttaaa gttgaggagt
    10861 aagagatgcg tttaaatact ctgtctccgg ccgaaggctc caaaaaggcg ggtaaacgcc
    10921 tgggtcgtgg tatcggttct ggcctcggta aaaccggtgg tcgtggtcac aaaggtcaga
    10981 aatctcgttc tggcggtggc gtacgtcgcg gtttcgaggg tggtcagatg cctctgtacc
    11041 gtcgtctgcc gaaattcggc ttcacttctc gtaaagcagc gattacagcc gaagttcgtc
    11101 tgtctgacct ggctaaagta gaaggcggtg ttgtagacct gaacacgctg aaagcggcta
    11161 acattatcgg tatccagatc gagttcgcga aagtgatcct ggctggcgaa gtaacgactc
    11221 cggtaactgt tcgtggcctg cgtgttacta aaggcgctcg tgctgctatc gaagctgctg
    11281 gcggtaaaat cgaggaataa gtagcagatg gctaaacaac cgggattaga ttttcaaagt
    11341 gccaaaggtg gcttaggcga gctgaaacgc agactgctgt ttgttatcgg tgcgctgatt
    11401 gtgttccgta ttggctcttt tattccgatc cctggtattg atgccgctgt acttgccaaa
    11461 ctgcttgagc aacagcgagg caccatcatt gagatgttta acatgttctc tggtggtgct
    11521 ctcagccgtg cttctatctt tgctctgggg atcatgccgt atatttcggc gtcgatcatt
    11581 atccagctac tgacggtggt tcatccaacg ttggcagaaa ttaagaaaga aggggagtct
    11641 ggtcgtcgta agatcagcca gtacacccgc tacggtactc tggtgctggc aatattccag
    11701 tcgatcggta ttgctactgg tctgccgaat atgcctggta tgcaaggcct ggtgattaac
    11761 ccgggctttg cattctactt caccgctgtt gtaagtctgg tcacaggaac catgttcctg
    11821 atgtggttgg gcgaacagat cactgaacga ggtatcggca acggtatttc aatcattatc
    11881 ttcgccggta ttgtcgcggg actcccgcca gccattgccc atactatcga gcaagcgcgt
    11941 caaggcgacc tgcacttcct cgtgttgctg ttggttgcag tattagtatt tgcagtgacg
    12001 ttctttgttg tatttgttga gcgtggtcaa cgccgcattg tggtaaacta cgcgaaacgt
    12061 cagcaaggtc gtcgtgtcta tgctgcacag agcacacatt taccgctgaa agtgaatatg
    12121 gcgggggtaa tcccggcaat cttcgcttcc agtattattc tgttcccggc gaccatcgcg
    12181 tcatggttcg ggggcggtac tggttggaac tggctgacaa caatttcgct gtatttgcag
    12241 cctgggcaac cgctttatgt gttactctat gcgtctgcaa tcatcttctt ctgtttcttc
    12301 tacacggcgt tggttttcaa cccgcgtgaa acagcagata acctgaagaa gtccggtgca
    12361 tttgtaccag gaattcgtcc gggagagcaa acggcgaagt atatcgataa agtaatgacc
    12421 cgcctgacct tggttggtgc gctgtacatt acctttatct gcctgatccc ggagttcatg
    12481 cgtgatgcaa tgaaagtacc gttctacttc ggtgggacct cactgcttat cgttgttgtc
    12541 gtgattatgg actttatggc tcaagtgcaa actctgatga tgtccagtca gtatgagtct
    12601 gcattgaaga aggcgaacct gaaaggctac ggccgataat tggtcgcccg agaagttacg
    12661 gagagtaaaa atgaaagttc gtgcttccgt caagaaatta tgccgtaact gcaaaatcgt
    12721 taagcgtgat ggtgtcatcc gtgtgatttg cagtgccgag ccgaagcata aacagcgcca
    12781 aggctgattt tttcgcatat ttttcttgca aagttgggtt gagctggcta gattagccag
    12841 ccaatctttt gtatgtctgt gcgtttccat ttgagtatcc tgaaaacggg cttttcagca
    12901 tggaacgtac atattaaata gtaggagtgc atagtggccc gtatagcagg cattaacatt
    12961 cctgatcata agcatgccgt aatcgcatta acttcgattt atggcgtcgg caagacccgt
    13021 tctaaagcca tcctggctgc agcgggtatc gctgaagatg ttaagatcag tgagctgtct
    13081 gaaggacaaa tcgacacgct gcgtgacgaa gttgccaaat ttgtcgttga aggtgatctg
    13141 cgccgtgaaa tcagcatgag catcaagcgc ctgatggatc ttggttgcta tcgcggtttg
    13201 cgtcatcgtc gtggtctccc ggttcgcggt cagcgtacca agaccaacgc acgtacccgt
    13261 aagggtccgc gcaaaccgat caagaaataa tcggggtgat tgaataatgg caaaggcacc
    13321 aattcgtgca cgtaaacgtg taagaaaaca agtctctgac ggcgtggctc atatccatgc
    13381 ttctttcaac aacaccatcg tgactatcac tgatcgtcag ggtaacgcgt tgggttgggc
    13441 aacagccggt ggttccggtt tccgtggttc tcgcaaatcc actccgtttg cagcacaggt
    13501 tgcagcagag cgttgcgctg acgccgtgaa agaatacggc atcaagaatc tggaagttat
    13561 ggttaaaggt ccgggtccag gccgcgaatc tactattcgt gctctgaacg ccgcaggttt
    13621 ccgcatcact aacattactg atgtgactcc gatccctcat aacggttgtc gtccgccgaa
    13681 aaaacgtcgc gtataacgcc tcgttttcca ggtttgttgg agaaagaaaa tggcaagata
    13741 tttgggtcct aagctcaagc tgagccgtcg tgagggcacc gacttattcc ttaagtctgg
    13801 cgttcgcgcg atcgatacca agtgtaaaat tgaacaagct cctggccagc acggtgcgcg
    13861 taaaccgcgt ctgtctgact atggtgtgca gttgcgtgaa aagcaaaaag ttcgccgtat
    13921 ctatggtgtg ctggagcgtc agttccgtaa ctactacaaa gaagcagcac gtctgaaagg
    13981 caacaccggt gaaaacctgt tggctctgct ggaaggtcgt ctggacaacg ttgtataccg
    14041 tatgggcttc ggtgccactc gtgcagaagc acgtcagctg gttagccata aagcaattat
    14101 ggtaaacggt cgtgttgtta acatcgcttc ttatcaggtt agtccgaatg acgttgtaag
    14161 cattcgtgag aaagcgaaga agcagtctcg cgtgaaagcc gctctggagc tggctgagca
    14221 gcgtgaaaag ccaacctggc tggaagttga tgctggcaag atggaaggta cgtttaagcg
    14281 taagccggag cgttctgatc tgtctgcgga cattaacgaa cacctgatcg tcgagcttta
    14341 ctccaagtaa agcttagtac caaagagagg acacaatgca gggttctgtg acagagtttc
    14401 taaaaccgcg cctggttgat atcgagcaag tgagttcgac gcacgccaag gtgacccttg
    14461 agcctttaga gcgtggcttt ggccatactc tgggtaacgc actgcgccgt attctgctct
    14521 catcgatgcc gggttgcgcg gtgaccgagg ttgagattga tggtgtacta catgagtaca
    14581 gcaccaaaga aggcgttcag gaagatatcc tggaaatcct gctcaacctg aaagggctgg
    14641 cggtgagagt tcagggcaaa gatgaagtta ttcttacctt gaataaatct ggcattggcc
    14701 ctgtgactgc agccgatatc acccacgacg gtgatgtcga aatcgtcaag ccgcagcacg
    14761 tgatctgcca cctgaccgat gagaacgcgt ctattagcat gcgtatcaaa gttcagcgcg
    14821 gtcgtggtta tgtgccggct tctacccgaa ttcattcgga agaagatgag cgcccaatcg
    14881 gccgtctgct ggtcgacgca tgctacagcc ctgtggagcg tattgcctac aatgttgaag
    14941 cagcgcgtgt agaacagcgt accgacctgg acaagctggt catcgaaatg gaaaccaacg
    15001 gcacaatcga tcctgaagag gcgattcgtc gtgcggcaac cattctggct gaacaactgg
    15061 aagctttcgt tgacttacgt gatgtacgtc agcctgaagt gaaagaagag aaaccagagt
    15121 tcgatccgat cctgctgcgc cctgttgacg atctggaatt gactgtccgc tctgctaact
    15181 gccttaaagc agaagctatc cactatatcg gtgatctggt acagcgtacc gaggttgagc
    15241 tccttaaaac gcctaacctt ggtaaaaaat ctcttactga gattaaagac gtgctggctt
    15301 cccgtggact gtctctgggc atgcgcctgg aaaactggcc accggcaagc atcgctgacg
    15361 agtaaccgga tcacaggtta aggttttact gagaaggata aggtcatgcg ccatcgtaag
    15421 agtggtcgtc aactgaaccg caacagcagc catcgccagg ctatgttccg caatatggca
    15481 ggttcactgg ttcgtcatga aatcatcaag acgactctgc ctaaagcgaa agagctgcgc
    15541 cgcgtagttg agccgctgat tactcttgcc aagactgata gcgttgctaa tcgtcgtctg
    15601 gcattcgccc gtactcgtga taacgagatc gtggcaaaac tgtttaacga actgggcccg
    15661 cgtttcgcga gccgtgccgg tggttacact cgtattctga agtgtggctt ccgtgcaggc
    15721 gacaatgcgc cgatggctta catcgagctg gttgatcgtt cagagaaagc agaagctgct
    15781 gcagagtaat ctgaagcaac gtaaaaaaac ccgccccggc gggttttttt atacccgtag
    15841 catccccact tatctacaat agctgtactc tttttgttca tcccctggag tatttatgtg
    15901 gttacttgac cagtgggcag agcgtcatat agcagaagcg caagcgaaag gtgagtttga
    15961 taacctggta ggtagcggcg aaccattgat actggatgat gattctcatg tgccaccgga
    16021 attacgtgcg gggtatcgct tgctgaagaa tgctggttgc ttaccgccag aacttgagaa
    16081 taggagggaa gcaatacagc ttctggatat tctcaaaggt atccgtcatg atgatccgca
    16141 atatcaagag gttagtcgtc gattgtcatt actgcaattg aagctgcgac aagctggatt
    16201 gagtaccgat tttttacgcg gcgattatgc tgacaagttg ttgaacaaaa tcaacgataa
    16261 ctagtggagt atgtatgtat cgcattggtg agctggcaaa aatggcggaa gtaacacccg
    16321 acacgattcg ttattacgaa aagcagcaga tgatggagca tgaagtacgc accgaaggtg
    16381 ggtttcgcct gtataccaaa agcgatctcc agcgattgaa atttatccgc catgccagac
    16441 aactaggttt cagtctggag tcgatccgcg agttgctgtc gatccgtatc gatccggaac
    16501 accatacctg tcaggagtca aaaggcattg tgcaggaacg attgcaggaa gtcgaagctc
    16561 gtatagccga gttgcagagt atgcagcgtt ccttgcaacg ccttaatgat acctgttgtg
    16621 ggaccgccca tagcagtgtt tattgctcga ttcttgaagc tcttgaacaa ggggcgagtg
    16681 gcgttaagag tggctgttga ttttttgcac tggcaggatt acactcgcgc cgttaaataa
    16741 ccaactggag tttttatgag ccgatatcag catactaaag ggcagataaa ggataatgcg
    16801 atagaagcat tactacatga tcccttattc cgacagcgag tagagaaaaa taagaaaggg
    16861 aaaggcagtt acatgcgaaa aggaaaacat ggcaatcggg ggaactggga ggccagtggc
    16921 aagaaagtaa atcacttttt taccactggc cttctgcttt cagacgcttg ttaagaacgg
    16981 ttattctgct ctttcagcaa atcacgaatt tctgtcagta atacttcttc tttagttggt
    17041 gcaggtgttg ctgctgctgg ttcttctttt ttccgattca gtttgttgat tagcttaatc
    17101 gccataaaga tggcaaaggc cactatcaga aaatcaaaga cgttttgaat gaagacaccg
    17161 taatgcatca caacagcagg gatatcccct tgggcctcgc gtagcgtgac agcaaactgt
    17221 ttaaagtcga tcccgccaat taataagccc agaggtggca tgatgatatc ggcaaccagt
    17281 gaagatacaa tcttcccgaa tgccgctccg ataatgacac ccactgccaa atccaccacg
    17341 ttcccgcgca tcgcaaattc gcgaaattct ttaataatgc tcatgttatt ctccctatga
    17401 agccgacaac cataagtcta acaaatgtta agccattttc ctgccaggaa tggatttaat
    17461 taatccttaa taatcaaagg gaaataataa ggcgtcatta gacgccttat taattacaag
    17521 aagaaaggac ttggctggaa gagtctttcg acgtcggtaa taaacttttt atctgtgagg
    17581 aacataatta cgtgatcgcc ttgctcaatg cgcagattgt cattggcaat catcacatcg
    17641 tttccacgta ccaccgctcc aataatcgtt cctggcggta gcttgatttc gtcaatgact
    17701 ctgccgacaa cgcgtgaggt gctttcatca ccgtgagcaa cggcttcaat agcttctgct
    17761 acgccgcggc gtaatgagga aacaccaaca atatctgctt ttcgcacatg gctaagcaac
    17821 gcagaaatag ttgcttgttg tggtgaaatc gcaatatcga tcacgctccc ctgaaccaga
    17881 tccacataag cgcgacgttg gatcaatacc atcacctttt tcgcacccat acgtttggca
    17941 agcatggcgg acatgatatt ggcctcgtca tcgttggtga cggcaataaa cagatcaact
    18001 tgatcgatat gttcttcggc cagcagttct tgatccgacg catcaccaaa aaagacgatc
    18061 gtattctgca acttttccgc cagttcggca gcgcgctgct gattacgttc gatgagttta
    18121 acgctgtaat ctttttccag acgacgcgcc agccctgcac cgatattacc gccaccaacc
    18181 agcatgatcc gcttatacgg tttttccagt cgctgtaatt cactcatcac cgcgcggata
    18241 tgctgtgaag cggcaataaa gaacacttca tcaccagctt caacaatggt cgaaccttgc
    18301 ggacgaatgg ggcgatcgtg gcggaaaatt gctgccacac gagtatcgat atgtggcata
    18361 tgttcgcgca tggtcgaaag tgcattacca atcagcgggc cgccataata ggctttaacc
    18421 acagccaggc tgactttacc ctcagcgaag ttcaccacct gcaatgcgcc gggatactca
    18481 atcagtcggt aaatattatc gataaccaac tgctctggtg cgatcagatg atcaatcgga
    18541 acagcatctg aatgaaatag cttatcggca tcgcgcacgt agtctggtga gcggatacga
    18601 gcgatgcgat taggggtgtt gaaaagtgag taggctatct ggcaggcaac catattggtt
    18661 tcatctgaac tggttacagc aaccagcata tcggcgtcgt cggcacctgc ctcccgcaat
    18721 acgcgtggat gagagccatg cccctgcacg actcgcaggt caaatttatc ctgtaaggtc
    18781 cgcagacgct caccgttggt atcgacaaca gtaatatcgt tgttctcgcc aaccaggttt
    18841 tccgccagtg tgccgccaac ctggccggca cccagaatga taattttcat cagtcgcgac
    18901 ccgttatctc atcacttttt gattagctta gcgtaaaaga agccgtcgcc ctcttcggca
    18961 ccgggcaaat tttgtttacc cggttgctct ggtgttcctg tttcgcaaag ttcggcatca
    19021 gcggtacgtt gcaaaaaggc tttaatctgc aggctattct cttccggtaa caccgaacag
    19081 gtggcataga ccagggttcc tccggatttt aaatgcgacc agatggcgtc gagaatttca
    19141 gactgcaact gcgcgagttc tgggatatcg cgatcgcggc gtaaccattt aatatccgga
    19201 tggcgacgaa ttacaccagt tgccgaacaa ggtgcatcta ataaaatgcg atcgaactgt
    19261 tgctcaccac accattggga agggtaacgg ccatcacctt gttttacggt tgccttcata
    19321 ccaaggcgtt ttaaattgtc gtaaacgcga gagaggcgct gctcatcaat atcgaccgcc
    19381 agaacttgcg cctctggagc aacctcaaga atatgcgttg ttttaccgcc tggggcggcg
    19441 caaagatcca gaatgtgttc accattttgt ggagcaagcc aggtcatgca accttgtgct
    19501 gaagcatcct gaacggtaac ccatccttct tcaaaaccgg gtagcgcatg aacaggtgca
    19561 ggtgtttcca gctgtacagc atcagggtaa tccgcatgcg ggaaaccttt cattcctgct
    19621 tcatccagca atgcaagcca gctgtcgcgg gaatgatgcg tacgattaac gcgcagccac
    19681 attggcggac gctggttatt ggcttcgacg atggattgcc actgctcggg atacgccttt
    19741 tgcagacgct tcagcaacca ggaaggatgc agataacgtg catcactggc attaaactcg
    19801 gctaataact catcttgctg acgctggaac tgacgtaata caccgttaat caatccctta
    19861 agttgcgggc gcttaattgc aactgcgcct tcaaccgttt cagccagcgc ggcatgaggt
    19921 ggaatgcggg tataaagcag ttgatacaaa ccaaccataa tcaggtaatg aacagtacgc
    19981 tgtttgcctg tcatcggacg ggccattagc ttattaatca gccagtctaa ctgtgagagc
    20041 gtacgcagta cgccaaagca taactcctgg agaagagctt tatctttatc ggaaactttt
    20101 tgctggagcg gtggcagaat gttgctcagt gattgccctt gctcaacgac ttgttcaatg
    20161 gcctgggccg ccatgctacg taaattacgt tgttttttca taagtataaa tataaagatg
    20221 cccggtaaga ccgggcttag aagagtggac tatcaggcca gacggttgcc cggtacaaac
    20281 cattcccgac gagaattcag gaggtcttgc acgctcatcg ctttcttacc tgcaggttgc
    20341 attgagagca gattcaggat gccatcacct gtcgctacct gaatgccttg tttattggct
    20401 tcaaggattg ttcctggcgc agcctttgtt gtcgtatcga tgacagatgc tttccagact
    20461 ttaaccggct gcccttcaat ttccagccag ctcattggcc atggattgaa agcgcgaatg
    20521 caacgttcaa gctgtgctgc cgaaagtgac cagtcaatac gcgcttcttc tttactcaac
    20581 ttttcggcgt aagtgacaag agtttcgtcc tgaacttctg gtttcgccgt gccgtctgcc
    20641 agttgtttca acgtagtaat aagcccttgt gggccaagct ctgccagttt gtcgtacagc
    20701 gtaccactgg tatcttctgc agtaatcggg caggagagct tataaagcat gtcaccggtg
    20761 tctaaaccaa catccatttg cataatggtc acaccagttt ctgcatcacc cgcccatagt
    20821 gagcgttgga ttggtgcagc accgcgccag cgtggcagca gtgaaccatg aacgttgata
    20881 cagccaagac gcggcatctc cagcacagct ttaggcagaa ttaaaccata ggcgacgacg
    20941 accataacat cagcctgcag atcggcgacc agttgctggt tttcttgtgg acgcagggaa
    21001 acaggttgaa aaacgggcag acctttgtcc tcagccagaa ctttaaccgg gctgggcatc
    21061 agttttttac cgcgtcctgc cggtcggtct ggctgggtaa acacgccaac gatgttatga
    21121 ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt caggtgtacc cgcaaaaata
    21181 atacgtagtg attctgacac gttaattctt atccttaagc ccgggctttc agacgatcca
    21241 gtttttcaac tttctgacga atacgttgtt gtttcagcgg tgacagataa tccataaaca
    21301 gtttgccgac caggtgatcc atctcatgct gaatacagat ggctaacaga ccgtctgctt
    21361 ccagttcaaa tggtttaccg tcgcggtcca gggcgcgaat tttaactttc tctgcgcgtg
    21421 gcactaaagc acgttgttca gggatcgaca ggcaaccttc ttcaatgcct gtttcgccgc
    21481 ttttttctaa aagctccgga ttgattaaca ccagccgttc gtcacggttt tccgaaacat
    21541 caattacaat gatacgttga tggatatcaa cctgggttgc cgccaggcca ataccttctt
    21601 ctgcgtacat cgtctcgaac atatcatcga cgatacgctg aatttctgca ttcacttctt
    21661 ctaccggttt agcaactttg cgaagccgct cgtccggaat atgtaacact tgcaaaactg
    21721 acataaatct ccagagatgt gttcaggagt tagaaagatt atttcttcta ttctagacaa
    21781 atccccctct gattgacagc atcactgacc aatcgcaaag attgctaagg ctgcttatgg
    21841 cagggagata aggatggtcg atacagaaat ttggctgcgt ttaattagca tcagcagctt
    21901 gtatggcgat gatatggtcc gtatagctca ctggctggca aaacagtcgc atattgatgc
    21961 ggttgtattg cagcaaacag ggcttacatt gcggcaggca caacgctttc tttcatttcc
    22021 gcgaaagagt atcgaaagct cactttgttg gttggagcaa cccaaccatc atttaatccc
    22081 tgcggacagc gaattttatc ctcctcaact tctggcgacg acagattacc ccggcgcact
    22141 gtttgttgaa ggagaactgc acgcgttgca ttcatttcag cttgccgtag tggggagtcg
    22201 ggcgcattca tggtatggcg agcgatgggg acgattattt tgcgaaactc tggcgacgcg
    22261 tggagtgaca attacgagtg gactggcgcg tggaatcgat ggtgtggcgc ataaagcggc
    22321 cttacaggta aatggcgtta gcattgctgt attggggaat ggacttaata ccattcatcc
    22381 ccgccgccat gctccactgg ctgccagtct acttgagcaa ggtggtgctc tcgtctcgga
    22441 atttcccctc gatgttccac cccttgctta caatttccca cgaagaaatc gcattatcag
    22501 tggtctaagt aaaggtgtac tggtggtgga agcggctttg cgcagtggtt cgctggtgac
    22561 agcacgttgt gcgcttgagc aggggcgtga agtttttgct ttgccaggtc caatagggaa
    22621 tccgggaagc gaagggcctc actggttaat aaaacaaggt gcgattcttg tgacggaacc
    22681 ggaagaaatt ctggaaaact tgcaatttgg attgcactgg ttgccagacg cccctgaaaa
    22741 ttcattttat tcaccagatc aggaagacgt ggcattgcca tttcctgagc tcctggctaa
    22801 cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa cgtgccggcc aacctgtgcc
    22861 agaggtagtt actcaactac tcgaactgga gttagcagga tggatcgcag ctgtacccgg
    22921 cggctatgtc cgattgagga gggcatgcca tgttcgacgt actaatgtat ttgtttgaaa
    22981 cctatattca cacagaagct gagttgcgtg tggatcaaga caaacttgaa caggatctta
    23041 ccgacgcagg atttgatcga gaagatatct acaatgccct gctttggctg gaaaagcttg
    23101 cggattatca ggaaggactg gcagaaccga tgcaactggc ctctgatcca ctctccatgc
    23161 gtatttatac ccctgaagag tgtgaaagac tggatgccag ctgtcgtggt tttctgcttt
    23221 tccttgagca aattcaggtg cttaaccttg aaacccgtga aatggtgata gagcgagtgc
    23281 tcgcgctgga taccgctgag ttcgacctgg aagacctgaa atgggtaatc ctgatggtgt
    23341 tgttcaatat tccgggctgc gaaaatgcgt accagcaaat ggaagaatta ctctttgaag
    23401 tgaatgaagg tatgctgcat taatcatttc tttaattcag cataagtagt tatggcgaaa
    23461 tcagcactgt tcacggtgcg taataatgag tcctgcccaa agtgcggggc tgaactagtt
    23521 attcgatccg ggaaacacgg tccgtttctt ggatgctcac agtatccggc gtgtgactac
    23581 gtccgtcctc tgaaatcttc agcggatgga catattgtca aagttctgga ggggcaggtt
    23641 tgccctgcat gtggcgcaaa tctggtatta cgccagggac gctttggtat gtttattggt
    23701 tgcagtaact accctgaatg cgaacatacc gaacttatcg ataaaccgga cgaaacagca
    23761 attacatgcc cccaatgccg gacgggccat ctggttcagc gccgctcccg ttatggcaaa
    23821 acatttcact cctgtgatcg ctacccggag tgtcaatttg ccattaactt caaacccata
    23881 gctggagaat gccctgagtg ccattatccg ctactcatcg aaaagaaaac cgcgcagggt
    23941 gtaaaacact tttgtgccag taaacaatgt ggaaagccga tttcggcgga ataataacgt
    24001 gaataataac ctgcaaggag acgctatcgc agctgcgata gatgtcctca atgaagaacg
    24061 tgtcatcgcc tatccaacgg aagccgtttt cggtgtcggg tgcgatcctg atagcgaaac
    24121 agcagtgatg cgactgttgg agttaaaaca gcgtccggtt gataaggggc tgattctaat
    24181 cgccgcaaat tacgagcagc ttaaacccta tattgatgac accatgctga ctgacgcgca
    24241 gcgtgaaact atattttctc gctggccagg tcctgtcacc tttgtttttc ccgcgcctgc
    24301 gacaacaccg cgctggttga cgggccgctt tgattcgctt gctgtacgag tcaccgacca
    24361 tccgttggtg gttgctttgt gccaagctta tggtaaaccg ctggtttcta ccagtgccaa
    24421 cttgagtgga ttgccacctt gtcgaacagt agacgaagtt cgcgcacaat ttggcgcggc
    24481 gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat ccttcagaaa tccgcgatgc
    24541 cctgacgggt gaactgtttc gacaggggta acataatgga aacctatgct gtttttggta
    24601 atccgatagc ccacagcaaa tcgccattca ttcatcagca atttgctcag caactgaata
    24661 ttgaacatcc ctatggacgc gtgttggcac ccatcaatga tttcatcaac acactgaacg
    24721 ctttctttag tgctggtggt aaaggtgcga atgtgacggt accttttaaa gaagaggctt
    24781 ttgccagagc ggatgagctt actgaacggg cagcgttggc tggtgctgtt aataccctca
    24841 agcggttaga agatggacgc ctgctgggtg acaataccga tggtataggt ttgttaagcg
    24901 atctggaacg tctgtctttt atccgtcctg gtttacgtat tctgctcatt ggcgctggtg
    24961 gagcatctcg cggcgtacta ctgccactcc tttccctgga ctgtgcggtg gcaatcacta
    25021 atcggacggt atctcgcgca gaagagttga ctaaattgtt tgcgcacact ggcagtattc
    25081 aggcgttggg tatggacgaa ctggaaggtc atgagtttga tctcattatt aatgcaacat
    25141 ccagtggcat cagtggtgat attccggcga tcccgtcatc gctcattcat ccaggcattt
    25201 attgctatga catgttctat cagaaaggaa aaactccttt tctggcatgg tgtgagcagc
    25261 gaggctcaaa gcgtaatgct gatggtttag gaatgctggt ggcacaggcg gctcatgcct
    25321 tccttctttg gcatggcgtt ctgcctgatg tagaaccggt catcaaacta ttgcaacagg
    25381 aattgtcagc gtgaatcagg ccatccagtt tccggatagg gaagagtggg acgagaataa
    25441 aagatgtgta tgttttcccg ctctcgtgaa tggtatgcaa ctgacatgtg cgatctctgg
    25501 cgagagtctg gcgtatcgct ttactggaga tacgccagaa cagtggttag cgagttttcg
    25561 tcagcatcgc tgggacctgg aagaagaagc ggaaaactta attcaggaac aaagtgaaga
    25621 tgatcaaggt tgggtctggt taccttgatc cagatattcg tccttccatt tcacgtagtt
    25681 atttgcggaa tagcgtaacc cagccttctc ttcatcactt aacgggcgga tctgtttgac
    25741 ggggctaccg agatacagat atccgctctc cagccgttta ttttgtggga ccaggctacc
    25801 cgcaccaatc atcacatcat cttctactat tgcgccatca agtaaaattg agcccatccc
    25861 aaccaaaact cgattgccaa tggtgcagcc gtggagcatc accttgtgac caacagtgac
    25921 atcttcgcca atggttaatg ggttgccatc tgggttgtac gaggatttat gagtgacatg
    25981 caacatactg ccatcctgga tattggtgcg tgctccgatc tgtacataat gtacatctcc
    26041 acgaatcaca acgagcggcc agatccccac atcatcagcc aaacgaacgt caccaatcac
    26101 gacgctgcta tcgtcgatca ttacgcgctg accgatttgt ggaaaaagat cgcggtatgg
    26161 gcgtaaaaca tcagacatac tcacctcaac aataaatgat ttactaatga ctttgggtgc
    26221 attattggcc ttgtgcaagt cttttagcat gcaaaaaagc accgttttgt gtgcgaatgc
    26281 agcaaaaagg gtgaaaaagc aacaaacaga aaaaaagatc aaaaaagtac ttgtgcaaaa
    26341 aattgggatc cctataatgc gcctccgttg agacgacaac gtgaaacact tcacaagatg
    26401 gtcgtaacaa caaagagaaa aaatcctgaa
//
LOCUS       DAZFFE010000064        26365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFFE010000064 DAZFFE010000000
VERSION     DAZFFE010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.064"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            133..396
                     /gene="yhcN"
                     /locus_tag="ACHJ69_002852"
     CDS             133..396
                     /gene="yhcN"
                     /locus_tag="ACHJ69_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO5015803.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(451..723)
                     /gene="yhcO"
                     /locus_tag="ACHJ69_002853"
     CDS             complement(451..723)
                     /gene="yhcO"
                     /locus_tag="ACHJ69_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO5015804.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(815..2782)
                     /gene="aaeB"
                     /locus_tag="ACHJ69_002854"
     CDS             complement(815..2782)
                     /gene="aaeB"
                     /locus_tag="ACHJ69_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO5015805.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATCYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFLGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLTGMLHKYQHALTDS"
     gene            complement(2788..3720)
                     /gene="aaeA"
                     /locus_tag="ACHJ69_002855"
     CDS             complement(2788..3720)
                     /gene="aaeA"
                     /locus_tag="ACHJ69_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO5015806.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(3728..3931)
                     /gene="aaeX"
                     /locus_tag="ACHJ69_002856"
     CDS             complement(3728..3931)
                     /gene="aaeX"
                     /locus_tag="ACHJ69_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO5015807.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            4114..5043
                     /gene="aaeR"
                     /locus_tag="ACHJ69_002857"
     CDS             4114..5043
                     /gene="aaeR"
                     /locus_tag="ACHJ69_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO5015808.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(5171..6616)
                     /gene="tldD"
                     /locus_tag="ACHJ69_002858"
     CDS             complement(5171..6616)
                     /gene="tldD"
                     /locus_tag="ACHJ69_002858"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO5015809.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(6772..10572)
                     /gene="yhdP"
                     /locus_tag="ACHJ69_002859"
     CDS             complement(6772..10572)
                     /gene="yhdP"
                     /locus_tag="ACHJ69_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO5015810.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGVRPLVAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPVAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWPALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTQSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGHIDSDITIFGDTLTLTNGLI
                     DTGFARLTAEGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(10640..12109)
                     /gene="rng"
                     /locus_tag="ACHJ69_002860"
     CDS             complement(10640..12109)
                     /gene="rng"
                     /locus_tag="ACHJ69_002860"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO5015811.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(12099..12692)
                     /gene="yhdE"
                     /locus_tag="ACHJ69_002861"
     CDS             complement(12099..12692)
                     /gene="yhdE"
                     /locus_tag="ACHJ69_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO5015812.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADRQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSFHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(12701..13189)
                     /gene="mreD"
                     /locus_tag="ACHJ69_002862"
     CDS             complement(12701..13189)
                     /gene="mreD"
                     /locus_tag="ACHJ69_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO5015813.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(13189..14292)
                     /gene="mreC"
                     /locus_tag="ACHJ69_002863"
     CDS             complement(13189..14292)
                     /gene="mreC"
                     /locus_tag="ACHJ69_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO5015814.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGITQPTPQQPATGNAVTA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(14358..15401)
                     /gene="mreB"
                     /locus_tag="ACHJ69_002864"
     CDS             complement(14358..15401)
                     /gene="mreB"
                     /locus_tag="ACHJ69_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO5015815.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(15706..17646)
                     /gene="csrD"
                     /locus_tag="ACHJ69_002865"
     CDS             complement(15706..17646)
                     /gene="csrD"
                     /locus_tag="ACHJ69_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO5015816.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTND
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLCEIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMLHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGRVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLLISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRRRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            17798..18772
                     /gene="yhdH"
                     /locus_tag="ACHJ69_002866"
     CDS             17798..18772
                     /gene="yhdH"
                     /locus_tag="ACHJ69_002866"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO5015817.1"
                     /translation="MQALLLEQQDDKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            18960..19347
                     /locus_tag="ACHJ69_002867"
                     /pseudo
     CDS             18960..19347
                     /locus_tag="ACHJ69_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240072.1"
                     /note="in Escherichia coli this inner membrane protein was
                     found to anchor the periplasmic catalytic oxidoreductase
                     YedY; sulfite oxidase activity not demonstrated; contains
                     heme; frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
     gene            19752..20222
                     /gene="accB"
                     /locus_tag="ACHJ69_002868"
     CDS             19752..20222
                     /gene="accB"
                     /locus_tag="ACHJ69_002868"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO5015818.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            20233..21582
                     /gene="accC"
                     /locus_tag="ACHJ69_002869"
     CDS             20233..21582
                     /gene="accC"
                     /locus_tag="ACHJ69_002869"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO5015819.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRSDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(21674..22720)
                     /locus_tag="ACHJ69_002870"
     CDS             complement(21674..22720)
                     /locus_tag="ACHJ69_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFO5015820.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGVVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDEGETLGLHGRLAATPAEEVGYRTERTDDSIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVMRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSVTPRTL
                     HAAEGLASHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEISALKAEIAGYR"
     gene            complement(22717..23679)
                     /locus_tag="ACHJ69_002871"
     CDS             complement(22717..23679)
                     /locus_tag="ACHJ69_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO5015821.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKENGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIETFIQRHDMSIREAAQ"
     gene            complement(23701..24690)
                     /locus_tag="ACHJ69_002872"
     CDS             complement(23701..24690)
                     /locus_tag="ACHJ69_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5015822.1"
                     /translation="MTEKTLASPKPSEIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFVLLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIPIPIWILIILLLINHFILTKTTFGRKIYITGGNKEAAIYSGI
                     NVTRLKIKVFMITAVLAGINGMILASRLYSGQPNSALSYELDAIAAAVLGGTSLNGGY
                     GTVVGTVIGALTIGVINNGMNLMNVPYFYQMVVKGLVILVAVYFDVRNKRKRS"
     gene            complement(24691..26190)
                     /locus_tag="ACHJ69_002873"
     CDS             complement(24691..26190)
                     /locus_tag="ACHJ69_002873"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO5015823.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEKPDENVPAKLEVKNLSHPSLFKEV
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYHGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
BASE COUNT         6597 a         7100 c         6310 g         6358 t
ORIGIN      
        1 atttttaaca cttagtatca actgaaacag ttagcgtggt attaattagc tcaataatta
       61 gtgtatactt gattttgtga tatgggtcac gaaacaaagg cccagctaaa agattatgtc
      121 gaggtaaaaa tcatgaaaat caaaaccact gttgctgcat taagcgtact ttctgttctc
      181 tctttcggtg cattcgctgc cgactccatt gatgctgcac aagcacaaaa tcgtgaagca
      241 atcgggaccg tatccgtaag tggtgtggcg tcttcgccaa tggatatgcg tgaaatgctg
      301 aacaaaaaag cggaagagaa aggcgcaacg gcctaccaga ttactgaagc tcgtagtggt
      361 gacacctggc acgctacggc tgaactgtac aaataaaccc tcatcgtctt gtccgacgat
      421 attgcccccg gttcgggggc tttttttgcg ctaatgacga acattaaaac gcaaatgccc
      481 ttccagctct tcctctgcct catcaaacag caatattaac gcgccaaaac ggcgacgcgt
      541 tttctctccc agatgaacaa attcaatctc aagtggtagc ggcaggacat cgttcattaa
      601 cacatcccat agtgagtcga gatcgcgtac cttatctttc gccagaccaa aggtttggct
      661 aaagtcacga taaaaatcct cctgactctc aatctcatca aaatcaaagg tataaatatt
      721 catctgttgc caccgtcacg tttcgcggcg agttctgccg ttcatcgcta agtatagcca
      781 tgaaaaaacc gacgcttcgg gcgtcggttt tgacttaact atcggtcaac gcatgttgat
      841 acttatggag catacccgta agccgatgta ctggttccgt cacctgcggt ggcgcttgcc
      901 agatgcgtag cttttcctgg tagatttcca gttcttccag caactggcca aagtaccgac
      961 gacgtttatc atcgctacgg gcagatatca catggtctgc tgtgcggcgc atttgccggt
     1021 gaaacgccga taaatcctcg ttaaccggaa tcggcgcatc acgcaggcgc tggtgcgcga
     1081 taatcatcgt cagcgccaga cgaaatttcg gcaaatcccc taggaactta ttcatcagca
     1141 aaaacagctg ctgataaagt gccggaaggt gattctcttt acgacgtgcc acattggtag
     1201 tcatcgcgga aacagcggca gaaacaaact gattaagcag tacacgtcca gtcctgtcgc
     1261 gcgatttatc ccgcaccagc aaaataacgg tgaacgcgag cacacagccg acgatttgcc
     1321 ctaatgcgct gtcgagaaat tgactgaaat ggaaagtcat cgggttatcc agcacgataa
     1381 tgtttatggt gctggccaac gcccccatcg agcccaatct ccgtttctgt acttctatac
     1441 cgaggaaaaa tcccagcact gccaggctaa tacacagcag caacatgctc tgttgggtat
     1501 tagggataat caccaaaaag tagagcagcc ctaacggtag cgctgccagc gtcccgtaga
     1561 taaagtcgat cgccaccatg cgtggattcg gcaaacgcat tgccagtgac gtcactaccg
     1621 caatcatcac cattgcacca ctgccggaag tccagcccgt ccacagccag aaaagcgtgc
     1681 ccagaatgca agaaagtgtg gttcgccaga agttaaccat tgcatgatga cgttcggctg
     1741 actctacttt tacttccggt tcgccttgca ggatctcttc ttcggtggcg ttgatttttg
     1801 tgttactgat aacgccgcgc ttgagaagct gataacacgt tgccgccgcg acccagctat
     1861 aaatggtgac aggcgtttcc cgttccccgg tccaggcgat cactctccgc aggcgtttga
     1921 gctgcttgtg gacgtcctgc gcagtttcta ccggtgtgtc aaaaaattcg cggaaagtat
     1981 cagtgatcaa ttccgggcgc gtattctgaa taagataagt ttcgcaggat tgggtaatca
     2041 gcgtcagcga tagcgtattg atcgctttta aacgtcgatt ggcccgcgcc cagcgggaag
     2101 attccatatt caggttgctg cgcatacctt gtaacgccgt agtacgtcgc accagatcgc
     2161 cccaggcttt atcgacaact tcaccatcgc catgcttgat acagagttgc attaattgat
     2221 attgcgcaac cagcaaactt tccagctctc gatccacttc ttgtttgatc gatcgcggag
     2281 aaaagagcaa atccgccata attgcacaca caataccgat aacgatctcg ctacaacgtt
     2341 cgacggcaaa ctgcggcgta agcaatggtt ccggctgaat ggtgatcaca atgatcagcg
     2401 cggtataacc ggccagcccc cacgcatacg agttttctat tcgtaccagc gaggatatcc
     2461 aggtacaaaa accggcccag atacagcaca ccagaatcat caataatggt gctcggatca
     2521 tcgcaatgat gattaccagt ccggcaatac agccaataaa tgtgccgatg atgcgcaaaa
     2581 agccacgata gcgaatagcg ccagaatacg gttcacctcc cgcagcaaag gccggaccgg
     2641 cggcaacaat cgccgctgtc agtaccgccc agcgtggcgt ttccagctgg aagtgaaagc
     2701 caacaaacag cgccagtaca atggcggtcg ccagttttac cgcaaagcga atatgttgat
     2761 tagcaatgga gaaaataccc atcgtgatta accaaactca cgcaggcgat gagccatttt
     2821 acggaagaac gaatcctgac tttcgtcgcg atcttgtttg ccagtgacta ccaccgtagc
     2881 agtggtgccc gcaggccaga tgttctcttg ctggttgtcg agacgaatac gtaccggaac
     2941 acgttgcgcc aaacgtaccc attcaaggtt agagtcgatg gtcgccatcc ctttgtcgtc
     3001 acgcgtgctg ctggcattgg tgacccctgc ggcaacacta tcaacagtcc ctttcagcac
     3061 tttgttactg ccaagcggcg tgatctccgc acgataccca gggcgcaccc cttccagctt
     3121 agtttcttcc atataggcca gtacatagaa ggagttctgt ttcaccagcg caaccgccgt
     3181 tgatcctcga gtaataaact caccggtata gacgttgagg ttggtcaccc agccatctgc
     3241 tggcgcgcgg atgactgtac gttcaagatc cagttttgcc agatcgcggg tcgcctgcgc
     3301 tttcgctaac tgatgcagaa ctgtttgtag tacgttgttc gcctggtcga tctcttcgcg
     3361 agacatcgcc tgcacaccga gacggttacg acgtccggcc tcctggcgtt tctcctgtgc
     3421 cagtacctga taataagcaa catcggcttg cgcttcctca agcgcctttt gatagcgcgg
     3481 ctggtcgatg gtgaacagta cctgtccttt tttcaccagc tggttatcat gaacattcac
     3541 ctgggtaatg agtccagaaa cgtccggcgc gatcgcaacg acgtcagcgc taaagcgcgc
     3601 gtcacgcgtc cacggggatt cggtgtaata gacccaggca ttaaaaattg cgatgaaggc
     3661 cagaatgact aatacgaccg tgatggccgt acgggagaat tttcttatta gtgttttcac
     3721 ttcaacctca aacgaacagt cgcgatatca aataaaacaa gcagcaatag agcgcggtgt
     3781 tgaacaacgc cggatgccag acaaagtcgt agatacctgt tggcacaagt acccggcgca
     3841 ccagccagaa aatcgccagt gataaaagca attcgaaaaa tatcggtggg aaggacagcc
     3901 caaacaccac gataacggga aacagactca tgttgacctt ggttgtatag agagagcagg
     3961 cgttattatt ttcagcatct gtcgccgcag agaagggcat ggaaagccgg gcgagagcaa
     4021 cattgctgta gattgatatt taatatatta acgtaactgt tatgctgtta tctatattat
     4081 gtgatctaaa tcacttttaa gtcagagtga ataatggaac gactaaaacg catgtcggtg
     4141 tttgccaaag tagttgaatt tggctctttt accgccgccg ccagacagct acagatgagc
     4201 gtttcgtcca tcagtcagac ggtgtcaaaa ctggaagatg agttgcaggt aaagctgtta
     4261 aaccgtagca cacgcagcat tggcctgacc gaagccggta gaatttacta ccagggctgc
     4321 cgtcggatgc tccatgaagt gcaggatgtt catgagcaac tgtatgcctt caataatacc
     4381 cctatcggga cgctacgcat tggctgttct tcaactatgg cacaaaatgt tctcgccggg
     4441 ctgacagcaa aaatgctgaa agaataccca ggtttgagcg tcaatctggt taccggaatt
     4501 cctgcccccg acctgattgc cgacggtctg gatgtggtga tccgcgtcgg cgcgttgcaa
     4561 gattccagcc tgttttcccg ccgtctgggc gcgatgccaa tggtggtgtg cgccgcaaaa
     4621 agctatctca cacaatacgg cataccagaa aaacccgccg atttaagtag tcactcatgg
     4681 cttgaataca gcgtgcgacc cgacaatgaa tttgaactga tcgcaccgga agggatctcg
     4741 actcgcctga tcccgcaagg gagatttgtg actaatgatc cgatgacgtt ggtgcgctgg
     4801 ctgactgcag gcgctgggat cgcctacgtg ccgttgatgt gggtgatcaa cgagatcaat
     4861 cgcggggaac tggagatcct gctaccacgt taccagtcag atccacgccc ggtttatgcg
     4921 ttatataccg aaaaagataa gctgccgctg aaggtacaag tcgtgatcaa ctcgctaacg
     4981 gattattttg ttgaggtcgg taaattgttt caggagatgc acgggcgcgg gaaagagaag
     5041 taattaatat tgtactgtta cgttgtacaa acctgtgcca acggggtccc ctcaccctaa
     5101 ccccagaggg gcgaggggac cgttcgtaca cggagaagga ttaattatcc ttctgaaaat
     5161 actgaaatta ttacgcggta ccgccaactg tcaggttatc aactttcagc gttggctggc
     5221 ccacgccaac cggcaaactt tgcccttctt taccgcagac acccacgccg ttatccagtt
     5281 tcaggtcgtt gccaaccatc gaaatctgct gcatggtttc gataccggaa ccaatcaacg
     5341 ttgcgccttt caccggctta gttactttac cgttttcaat aagatacgct tctgaggtgg
     5401 agaaaacgaa tttgccggag gtgatatcca cctggccgcc accaaagttc ggtgcataga
     5461 taccgtactc aacggattca ataatttcct gcggggtcga tttacccggc agcatatagg
     5521 tgttggtcat acgcggcatg ggcagatgag cgtaggattc acggcgaccg ttgccagtcg
     5581 gcgtcatccc catcaaacgc gcgttgagtt tatcctgcat gtagcctttc agaatgccgt
     5641 tctcaatcag cacgttgtac tggcctggcg taccttcgtc atcaatcgcc accgaaccac
     5701 ggcgatcgac catcgtgcca tcatcaacca cggtacacag ttctgaagcc accaactccc
     5761 cgacctgtcc actaaatact gaagtgccac ggcggttgaa gtcgccttcc agaccgtgac
     5821 ccaccgcttc atgcaacagc acgcccggcc aacctgcgcc aagtactacc ggcatggtgc
     5881 ccgctggtgc agcaacggca gaaagattga ccagcgccat acgcactgct tcttttgccc
     5941 acgcatccgc acggacttcg ccgtcgagat cagcaaggaa gaactcataa ccaaaacgac
     6001 cgccgccacc actggcaccg cgttcgcgtt tgccatcttc ttcgaccaaa acgctcacgg
     6061 aaagacgcac cagcggacga acatccgccg ctagtgtgcc gtcggtggcc gcaaccagaa
     6121 ttaattcata gacaccactg aggctggcag tcacttcctg tacgcgcttg tccgcttcgc
     6181 gggcaacctt atcgacgcga cgcagaatat ccaacttctc ttcacggctc atgctttgca
     6241 gcggatctac cgaggtatat aacgggctat gctctaccgc gcccagcgtc tgtactttgc
     6301 catcaccact atcacggacg atggtgcgcg ccgcttgtgc actctgttcc agcgccagca
     6361 ggctgatttg gtcagcataa gcaaatccgg ttttttcacc actgattgca cgcacgccaa
     6421 cgccctgatc gatgttgtaa gaaccatctt taataatacg gtcttctaaa acccaggatt
     6481 cgtgatagct cgactgaaaa tagagatcgc cataatcaag gcgacgttcg gccagttgac
     6541 cgaggatcgc gaacaagtcc tgatgtttca ggccgttcgc cgctagcaat tgttcactta
     6601 ccaggttcag actcatcgtt tttgctactc gttagttact gcagtagagg attttttacg
     6661 gctgccggat gcggcgtgaa cgccctgtcc ggcctacggt tatgttctgg ctcgttggcc
     6721 tttggcaacg attatcatat gagattgggg caattacgcg ccctcgtcaa atcattgcgc
     6781 tttttctttg cgcggttgtc gcaacacttc gttgatttgc ggatcgtcca gcggacccga
     6841 aatgtgatag cgcaaaatgg agactttgct ccacagcggt cccagcactt tactggcggc
     6901 aaacactgcc gcgccaacaa tggggttaac cgcaaaggcc gcagccacgc ccaccgtcgc
     6961 agaaatctct ggtgcgacaa ccgcttccat attcaggtcg cgacgtacca gatttaccga
     7021 ccctttcata gcgatgtccg cttccaggcc atccaccagc gtgtcgtcgg tgtgcataac
     7081 gccgtcttta atccacgcgg tgctgcgaat ggagtcaaaa tagaaccctt cgccaaaagt
     7141 gtctctgaaa tcaaaacgca gcttacgcat cagggcatct acgctcaata agcgcagcaa
     7201 ttgcccggca tgtccggtat tgatttcggt aatttcgcct ttacccagtt gagtatgaat
     7261 gatgccattc aacgtcgcct catctggctg ccagggtgct ttgcgccagt gtaaatcgta
     7321 atccacatta aatgacgact ggcgtatggg cgtcgtgaca ccaaaaaatt ctgcggcggc
     7381 atcaattttc tgcccgcgca gttttccttt cagcgaggtt cgttcattcc ccggattgtt
     7441 aacccattca ccctcggcag tgagccgcgc aaaaccagta tcaatcagtc cattagtcag
     7501 cgttaacgta tcgccaaaaa tggtgatatc actgtcaatg tgaccgaatt tttgccccca
     7561 gaaccagcac tctgcgcatc gtatttgtgc gtctggccag ccacggaagt taatgcgttc
     7621 cgttgtcggg aaaggtgatg actgtgttga atcaccacga gttttcgcca cgcttgggtt
     7681 gtaataaaga tatttgatat tcgccagcca cggtgcgtta ttacgcatcg ccagcgtggc
     7741 gttgatttca cgcccttgcg cctcaaccag ggtgccattt gccgtcggtt gcgaaacaat
     7801 actcaggtta ttccattgct gatttcccag cgacaacata ggcgtacgta acgttatgtg
     7861 ttgtgggaaa ctcgctgcac caccgacact ctccgccgcg cctttctgga acagggccgg
     7921 ccactcggca ccattcatcg gcggcatatt gagttcaacg ccactttgtt ccggcaacgg
     7981 cgggagcgtt ttactgtctg ccgcccaaat agcacgatcg agcgtcagct tttgaccgag
     8041 caaccagcgg ctattgaaat ggttatccgc accagcctgt ccggttaatt caaagctgtt
     8101 gagattgcca tcaaccttaa cgtttaccgc tagtggttca cccgcaggtt tggctaacgg
     8161 tgaaggtaag tgactgctca cattcttcaa atcgccgttc agctctacgt tataggtcgc
     8221 gccagcatga taaggcagat caatgcccac tttaccatcc cacgccacgc tgccactcaa
     8281 tgcttcgttc accgctacag gcagaacgcc ggttttcgcc ggttgccagt taccgttgag
     8341 gtttaccgct acctggtagg cttttgcccc ttccttggtg gaaaaatcca cgttcaacgg
     8401 ctgattaaac cagcttgctg tcagtggttc actttgcaga tcgccattga taaagctgaa
     8461 tttaccgctc aaatttttca gggtgctgtc gagtggtttg ataaacagac tgttattacg
     8521 cagcgtcact tcacctttcg cggttaccaa ctcgccgttc agcgggatat caagatgtaa
     8581 gcgagcattc acatcgccgt cgagctggag ttcttgcagg gtcgcaccca gggaatcttt
     8641 cagcggtgtc tcatcaaagt aagggccaac ggctttaccc ggacctttaa tgtcagcgtc
     8701 aatcagcagt ttttcttttg agtagtcagg gatcactgcg gtgagattac tcgcgcgcac
     8761 gccgcccaga ttaacgccat cggttttcat ccataaaccg tcgttaataa agtccagttc
     8821 aatatcaagg ttagttaatg caggccagtc cggctggaag gcaaacttcg cgttgcgcag
     8881 cggcaccagc acttcaaact gaccttcgtt gtgtttatag gggaagagtt gcggattgcc
     8941 accataaacc agcgtcgcgt tatccgcttc accgccctga atcgcgccac ttaagtaatc
     9001 aaccaggtct ttacccatca agttttccgg gaaatagcgc caggcttgtg aaccatcatc
     9061 ggtactgatg ccagccagaa tacccagcca gggttcatcg ttagcaggtt gcaggtaacg
     9121 aaaaccgccg cgcgcatgga cggctttggc tttaacgtca atattacgcc catccagctg
     9181 gaaacctttg tcattgttca gccagcttat cgttgcttgg ccgtcggcga tttccagtgg
     9241 cgcacggaat accgtttcgt aaggcatctt tgcctgcttc atcgacgcag taagcaaacc
     9301 attttctacg ctgccggaaa gcgtcccgga gaagtgttcc gcacccggta ataatttcca
     9361 ttgcttccag gccagatcgc tccacgatgc ctgaaaacgg gtcttgtctg ccgcctgaag
     9421 cgggatatcc agcgccagag tgttaatctt gccgctcggt tgtgtggagc gccaaacatc
     9481 acccagtgca ggtgaaagtt tcgcgaccag cgggcgtacg ccctccaggc ctgccagctc
     9541 cagattactg gcgcgaatcc ggagttcgtc actgcgttta ttgtctttgc cgccaacgtc
     9601 ctgttccggt atccaggcca gcgtcaatgc tccgctcggc cagggtttgc cgtccatcgt
     9661 gatccgtgta tcgggaatag agaactgcca tcccggattt tcacgcgtaa tatgcgcagt
     9721 cagattatcc accgacagcg tatgtgtttc cttctcgccc aaccagctgg caccgccctg
     9781 tttcagccag acgtcaccgc cggttacatc gcctttgtcg atcgtcatcc agccttcaag
     9841 ggagaactgt gccgtttcca gcgcaatatt gtcctgcatc catttaccga gccacggctt
     9901 caggtcgatg tcatccgcct ggagccagac gcgaccattg cttaacaacc cctcatcatc
     9961 gcgcaaatcc atacgcacct gcatcacgcc gtgctgtccg gtaaggctgg agaggcttac
    10021 caggccttcc gcacggtgtc gacgtggatc gttcagccag gtgagctgtg ggatcgccag
    10081 ctcggcgcgc tgaccggatg gcgtcaggaa actgacttca ctgtcgcgga gatcgaaatg
    10141 gtcaaattga cgaagaaaca gatcgctgat gtgactggct tccaggctat cattaccacc
    10201 gccgctggtg ataggagtgt tggtgcgaaa gcgcagctgc cagaaagtga ggtcgcgaaa
    10261 ctgccagcgc atatgtaaca ggctctgcca gacatccagc gcaagagtaa cgcgtttaac
    10321 cgaaaattcg ccgccatctt ttagttctgc acggatgtcg tgcgcttcaa gcgtcggacc
    10381 aaaattctgc cagctggctg agagctgact ggcttctacc ggcatgccag tcgcggattc
    10441 tattttgttg aggatttccg gacgccaggc gtcaagatgc ggtaaagcaa tacgcaggcc
    10501 gctaaccagc agggcagcga tcacaacgag cgcggctcca gtaagcagta aaatccccgg
    10561 caatcgcctc acccgtcact ccttgtctgc taaaaatgtg actcaaaaac cctttgccgg
    10621 atggcggccc agcatctgtt tacatcatta cgacgtcaaa ctgctcctgg ttatagagcg
    10681 gttcaatttg tactttaacc tgtttgccaa cgaaaatttc cacttccgcc agcgagtgtg
    10741 actcttcgcc tttcaaggct tcggctactg ccggagaagc atagaccagg aaacggtcgg
    10801 agtcgtaagc atggtggaca cgaacaatct cgcgcatgat ttcatagcat accgtttcca
    10861 cggttttcac cgttccgcga ccgtggcagg ttgggcattc gttacacagt acgtgctcaa
    10921 tgctttcgcg ggtgcgttta cgcgtcatct ccaccagccc cagcgccgaa aaaccattaa
    10981 cgctggtttt cacccgatct ttgctcagcg cctgctccag cgagtgcagc acgcggcgac
    11041 ggtgatcttc attattcata tcgatgaaat caataatgat aatcccgccc agattgcgca
    11101 accgtaactg gcgagcgata gcctgcgtcg cttcaatatt ggtattgaaa atggtgtcgt
    11161 ccagattgcg atgaccgaca aacgctccgg tattgatgtc cacggtggtc atcgcttcgg
    11221 tctggtcgat aatcagataa ccaccggatt tcagttctac tttgcgttcc agcgctcgct
    11281 gaatttcgtt ttcgacatca aagagatcga aaatcggctg gcgtccggtg taatgctcca
    11341 gcttgctggt catctcggga atgtactccg aggtaaactc cagcaatgct tcgtaagtca
    11401 ggcgtgagtc aacgcgaatg cggtcgagtt cggcgtcggc gaaatcacgc agaacacgct
    11461 gcgccagcgc cagttctccg tacagctgat aacgggtctg cgggcgtttt ttacgctcca
    11521 taactttggt ccagacgcgt ttcagataag cggcatcgga ggccagttca gcctcgccaa
    11581 ccccttccgc tgcagtacgg atgataaacc cgccctgctc gtcgcaatac tctgcgacca
    11641 cttttttcag gcgttcacgt tctgattcgc tttcaattcg ttgggatacc ccaacgtgag
    11701 aagcccctgg cataaacacc agatagcgag aaggcagcgt gatatcggtg gtcaggcgcg
    11761 cacctttagt gccaagcgga tctttcacca cctgcaccat cagatcttgc ccctgacgga
    11821 ccagttccga gatgtcgcgc accgtgaatt gcttttgttc ttcacccgcc acacattcag
    11881 tgtgcggcat gatgtcagat gcatgaagaa acgcggcttt atccagccca atatctacaa
    11941 aagccgcctg catacccgga agtacacgac ttacacgacc cttgtagata ttgcctacta
    12001 ttccgcgtcg cgcctcacgt tcaatatgaa tttcctgcag aataccgcca tcaatatacg
    12061 ccactcgagt ttccgaaggc gttacgttta ccaacaattc agccgtcatg tttatccctt
    12121 ttctcacgca gtgcgttaaa attacttaat aattcatacg tttcaaccag cggtaagccg
    12181 actacggcgt gaaagctgcc atttatcttc ctgacaaaac agccacccag cccctgaata
    12241 ccgtatgcac ctgctttatc taacggttcg ccgctggcga catagcccgc gatgtcttcg
    12301 tctgttaacg ttctgaaagt cacatcggtg accaccaggc aatcgagaat gtgctggcgg
    12361 tcagccaacg ccactgccgt catcacctga tgggtctgac ccgataattt gcgcaacatc
    12421 tgcgccgcat gctctgcgtc gcgcggtttc tcgagcactt ctccgttcag gataacgata
    12481 gtatccgcac ccagcaccgg gagatcctgc gccgtttgcg cgacacccgc ctgtgctttc
    12541 tcgcgcgcca gacgcacaac atactgctgc gcgctctcct gcggctgacg ctgctcctca
    12601 atgcccgtaa caatacgttc aaaggtcacg ccaagttgcg caagtaactc ctgacgacgc
    12661 ggagaaccgg aagctaaata cagagaagtc atagaaacct ttattgcact gcaaactgct
    12721 gacggacttt acgcatcagc aagaaaatcc acggccagag caccccattg actacactac
    12781 tccagaacac ttccggtctg aaagagacgt taatcactaa aaactctgcc cagaaaacaa
    12841 taatatccac caccagcgaa agcaacatga cgaccagcgc ctgctgccat aatgcgaggt
    12901 tgcggaaaag ctggtatttc agcgccacca ggtaagcaat gatgctcatc gccaatacgc
    12961 gtacgccaag cgtcgagccg ctgatcagat ccagtatggc acccatcaca aaacctgtgc
    13021 ccacatttac gcgatggggc aaggccagga tccaatacaa caagatgagt aacacccagt
    13081 ttggccggaa aacaatcagg ttatccggcc agggcatgat ttgcagcaac agcgcaatga
    13141 ggaaagagag ccagattacc cagcgtccct ggctacgata gctcgccact attgtcctcc
    13201 cggcgcacgc gcaggcggtt gagcaccact ttgcggcggc gtagcccttt gtggagagcg
    13261 attagcagcg ggctgtgtgg gcgcagcagg cgcagtaact gcatttcctg tcgccggttg
    13321 ctgcggagtc ggctgagtga tccccgttgc cggttcaggt aactttggcc ccatcgcgtc
    13381 tggcgaaggc aatacctgcg gcatcatctg catcaaacgt tcattagcaa cacgatgcac
    13441 ctcttccggc gtcatcgggt tagcgccgtt acgatccgcg ccccacagca gcagcagata
    13501 acgcaaacgt tgcagtcctg cagtcggacg cgcctgaatc acagtataag cgcgctgggt
    13561 atcgagtttt acggaagaga caaccgcgac cggatagcct tccgggaaac gaccgcccag
    13621 accggaagtc accagcacat caccaacacg aatatccgta ttcgctggca gatgctcaag
    13681 ctgcaaatca tccgtacaac cgttaccggc tgcaattacg cggatatcgt tgcgcagcac
    13741 ctggattggc agcgcgtggg tcgcatcaca aatcagcagc acgcggctgg tcagtttagc
    13801 gacggccacc acctgaccaa caacaccttt gtcgctgata accggctggc cttcataaac
    13861 gccattaacg ctacctttat cgataacaac ttgatcgcta taaggatcgt taaccgtgga
    13921 gataacctga gtcaccattt tctgctcatc ctgacgcagc ggggaaccca gtagctcgcg
    13981 cagacgcgcg ttctcctgtt tgtattgtcc aagcatcagc agttcactgt ttttcagcaa
    14041 cagttcctga cgtaacgccc ggttttcaag ttctagttgg tcacgcgagg ccagcgtctg
    14101 cgatacgcca tccagcaatt cacgaggagc attggaaaca aagtagaaag gactgacggc
    14161 ggtatccata taagtacgga tttgactgaa cgtccccagg cggctgtcgg caataataat
    14221 gccgagcgcc accagcaccg ccagaataag gcgaatctgt agcgacgggc cacggctaaa
    14281 aattggcttc ataagttatg cgtattctcg tatcagacca ggcagggtaa acagacactt
    14341 cccctgcctg catccgatta ctcttcgctg aacaggtcgc cgccgtgcat gtcgatcatt
    14401 tccagcgctt tgccgccacc gcgcgccaca caggtcagcg ggtcttcagc aacaacgact
    14461 ggaatgccgg tttcttccat taacaaacgg tcaaggttac gcagcagtgc gccaccaccg
    14521 gtgagcacca tgccgcgctc ggagatatcg gaagccagtt ccggcgggca ctgttccagt
    14581 gcaaccatta ccgcgctcac aataccggtc agcggttcct gcagtgcttc gaggatttcg
    14641 ttggagttca gggtaaaacc acgtggaaca ccttctgcaa ggttacggcc acgaacttcg
    14701 atttcacgga cttcatcgcc cgggtaagcc gaaccgattt cgtgcttgat acgttctgcg
    14761 gtggcttcac cgatcagaga accgtaatta cgacgcacat agttgatgat agcttcgtca
    14821 aaacggtcac caccaatgcg cacggaagag gagtaaacca cgccgttcaa ggagataaca
    14881 gcaacttcag tggtaccacc accgatatca accaccatag aaccggtcgc ttcagaaacc
    14941 gggaggccag caccaattgc ggcagccatc ggttcttcaa tcaggaagac ttcacgggca
    15001 ccagcgccct gcgcggattc acgaattgcg cggcgttcaa cctgggtcgc gccaaccggc
    15061 acacaaacca gaacgcgcgg gcttggacgc atgaagctgt tgctgtgcac ttgtttgatg
    15121 aagtgctgga gcattttttc agtcacgaag aagtcggcga taacgccgtc tttcattggg
    15181 cgaatggcag caatattgcc cggcgtacgg cccagcatct gcttcgcgtc atgacctact
    15241 gcagctacgc ttttcggtga accggcacga tcctgacgaa tggccaccac ggaaggctca
    15301 ttcaatacga tgccttgtcc ttttacataa atgagggtat tcgcagtacc caggtcaatg
    15361 gacaagtcat tggaaaacat gccacgaaat tttttcaaca tactaaggga taatcctgaa
    15421 agctggggcg gaaaagaaaa tccgcttact ttaccaacca cacgcagcag cgacaaggcg
    15481 caaaaatcat ctgctacggt gaaaattagt gcagttcgtt tcctttgtta caaatctctg
    15541 cctgagtcca gaaaggctta atgcatcagc agcattcctc gcctgtttgc aaccgcgtaa
    15601 ggtcattcat ctgcatatgt gctgcaacaa tctggcgagc agacaagcac actcccatga
    15661 gacgcagcgc gcgttattct acgtgaaaac ggattaaacg gcaggttaaa ccgagtatct
    15721 ttgtgaatat tttttcacgt tagtatcaag tggctgtgag gacgcgaaaa aatccccttg
    15781 cccacctgta acaccgcgct gaatcagggt ctgccactcg cttcgcgaac gtacgccggt
    15841 ggcgtaaacc tgggtgctgg tcccggagca ggcttccacc aggctttgaa ccagcagctg
    15901 gttctccgtt cgcttctcaa tgtttctgac cagccccgga tggagcttga gtaactcaac
    15961 attaagttct ttgatccaac tggtgcttac cagcgtcaaa ccagcctggt tgacggctac
    16021 ccgtacccct aaagcattca ctaaacgaat aacaggttgt aagcgactga tatgttgacc
    16081 aacatcggcc tctgcaagtt caataattat gcgtcttcgt tgcgattttt cacattgcat
    16141 taacgtatcg cgcagccaac gctgaaaacg cgggcgaatc agcgactcaa cggtaacctg
    16201 aatcgccaga ttttcctctg gccagtaacg caatagtgga ataagacggc tgattagcag
    16261 gcggtcatac tcttccgata aaccaaactg caagaccatc ggcatatact ctgccgagct
    16321 aacctcttca ttaccatcga agatgcggca catgagttca cgatgatgaa cccggccttc
    16381 gcgagtaacc gccggttttt gataaaggcg cgggccgccg cgactgagca tttgctcgat
    16441 aagcgtacgc cagcgaacat taccgcgtcc tttttcaggc aacgagtcat cgtaaatagc
    16501 ccagctattg ccaccctgca atcccgcatt acgcgtggca gactctgcat gttccattac
    16561 ctgctcggta tcctgaccac tacgccaggc acagatacca atgtggagca tatcgtcgcg
    16621 atcgagcatt ttattgttcg gcaaggtatc gacggctttg attaactgac tggcgatgct
    16681 ctctgcctct tttaacgtcc ggtgcggtaa cagcgcagca aaatcactgc ggtggtaacg
    16741 cgccagcagt gcgccagggt agcgcatcat aaatgtcgat agcagattcg tcagagagaa
    16801 gaactgttct tcaacctggc tgtgccccca ggtatcactc aacatattga aatccggcag
    16861 acgaatcatc atcacgatcc cgtgggtacc tactttctct tgatcttcca gtaacgttgc
    16921 taactgatta tcgaaaaaga gtcggttatt gaggccggtt ttcatgtcct gggcggcata
    16981 agagcggatc agcgtatcaa gacggctgtg ttgttcgcgt gcgttctgaa tttcacaaag
    17041 cagcgtatcc agcgcactgc tggttctggg cggccattca tagatggttc ccaacacatt
    17101 agagccacgc tcaccgttta agatacgagt agcccgggtt tccagcaatt cttgcccggc
    17161 aagttgccgt tgtaaccagc gtaccgccag gaagagcata agaatgataa agccaatcgc
    17221 ccccgtgagc ggcgcggtag tcattaaaga atggaaatag ttgcccatcg gatcctgata
    17281 aaccagacgc agcgacatcc ccggatgctt tatcaacgga acgctcagtt cacgaaacag
    17341 atcgttggtg ccaaccggac gataactacc atttctggcc agggtataaa cctgcttatc
    17401 accatggagc aggtctacac gaacgatatc tgccgacatc attaattcgg taatttgtgg
    17461 ccttaatgtg ctgaagtcat tcgacactag gtgggtatca atcgccgtcg ccaccgcctg
    17521 aacacgatga ctaaacttat actgaatggc gttgtagaaa cttagcgaac agcccagcaa
    17581 agtcacaaaa attgttaacc cggtgagcag cgtgacaaag gccgaaaatt tcgtcgttaa
    17641 tctcatcctt gtgttaactc cgatagtgag gaagcgggca tactagcaaa tcagatttat
    17701 ctcgcaattt attgcgcttc atcggctttg cttttccatt agcgagtata gtcttcagaa
    17761 attattttcc aatccatcat gcacatgagg accacttatg caggcgttac ttttagaaca
    17821 gcaggacgac aaaactctcg catcagtaca gactctggac gaaagtcgcc tgccggaagg
    17881 cgatgtcacg gttgatgttc actggtcgag cctgaactac aaagatgcgc tggcgattac
    17941 cggtaaggga aaaatcatcc gtaattttcc gatgattcct gggatcgatt ttgccggaac
    18001 tgtacgcacc agcgaagatc cgcgttttca tgccggtcag gaggtgttac tcactggctg
    18061 gggcgttggt gaaaaccact ggggtgggct ggcggaacag gcgcgagtga aaggtgactg
    18121 gctggttgcc atgccgcaag ggctggacgc gcgtaaagca atgattatcg gtactgccgg
    18181 ttttaccgcc atgctgtgtg tgatggcgct ggaagatgcc ggtgttcgcc cgcaggatgg
    18241 ggagattgtc gtgacgggtg ccagtggtgg cgtcggcagt accgccgtgg cgctgctgca
    18301 taagttgggt tatcaggtcg ttgctgtttc cggtcgcgaa agtacccatg aatatctgaa
    18361 aagtttaggt gccagccgta ttcttcctcg cgatgagttt gctgaatccc gtcctctgga
    18421 aaaacaagtc tgggctgggg caattgacac cgttggcgac aaagtgctgg caaaagtgct
    18481 ggcgcaaatg aattacggcg gctgcgtggc ggcctgtggt ctggcgggtg gttttactct
    18541 gccaaccacg gtcatgccat ttattctgcg taatgtccgt ttgcaagggg tggattcagt
    18601 aatgacgcca ccagcacgcc gcgcacaagc ctggcagcga ctggtcgccg atttaccgga
    18661 atcattctat acccaggcgg caaaagagat atctctggca gaggcaccga agtttgccga
    18721 ggccatcatt aataaccaga tccagggtcg cacgctggtg aaggttaact aaccatttag
    18781 caaggattaa taagagaggg aactcatttt gaaatcatta ttcaatcggt taacggaaaa
    18841 agcggttagt cggacagctt tcgtcgaaca ccttggtcag gaagttgtac aacatcatcc
    18901 aaactggaaa gtcatgattt cgactgacca taaattgatg cgcattgata cggggcacca
    18961 aacagccatt attgatacgc ctccgtcgcc tgttaggttt atggtgcttt gccttggcga
    19021 cgctacgctt aatctgttac tgatttctgg taccatgggg ggaataatct gattttattt
    19081 gattacaaat taaccactta aacctattga atgcggaaca ttgtgagtcg gttaattttg
    19141 cgtgctttag cattcacatc tttccggacg atgcagtgaa aattgggtaa tccccagcaa
    19201 ccgctgcgta atgtcgtcta tcttgtcgcg atcctggcat ccctacatta tttgtggtct
    19261 gtgaagatta tctcattgca gccccccatc ttcgcagggc tggctttaca gcttttcacc
    19321 ttacgttata agaagttccg tcgatgatgg cgctaatttc gtgaattgtg cggcttgttg
    19381 caaattacac ggtgttgaag gttatttaca tgttagctgt tgattatctt ccctgataag
    19441 accagtattt agctgccaat tgctacgaaa tcgttataat gtgcgacctc gtcctccctg
    19501 acgcagtttt tgcgctgcgg aaaaggtgac attggcgcaa cgaaggtata ttttgttttt
    19561 tgccggagga tagcagcaga tcgctgcaca atgtccgtca agtctaacat tgacactctg
    19621 gggcaaaata gaccggcgtc ccggcctgct ggaatttatc gctatgcata cagctgtcgg
    19681 ggcatacgct ttacagacgg cggtgaaacg cctgtcacaa tcacactaaa caaagagtac
    19741 ggaacccact catggatatt cgtaagatta aaaaactgat cgagctggtt gaagaatcag
    19801 gcatctccga actggaaatt tctgaaggcg aagagtcagt acgcattagc cgtgcagctc
    19861 ctgccgcaag tttccctgtg atgcaacaag cttacgctgc acctatgatg cagcaaccag
    19921 ctcaatctaa cgcagccgct ccggcgaccg ttccttccat ggaagcgcca gcagcagcgg
    19981 aaatcagtgg tcacatcgta cgttccccga tggttggtac tttctaccgc accccaagcc
    20041 cggacgcaaa agcgttcatc gaagtgggtc agaaagtcaa cgtgggcgat accctgtgca
    20101 tcgttgaagc catgaaaatg atgaaccaga tcgaagcgga caaatccggt accgtgaaag
    20161 caattctggt cgaaagtgga caaccggtag aatttgacga gccgctggtc gtcatcgagt
    20221 aacgaggcga acatgctgga taaaattgtt attgccaacc gcggcgagat tgcattgcgt
    20281 attcttcgtg cctgtaaaga actgggcatc aagactgtcg ctgtgcactc cagtgcggat
    20341 cgcgatctaa aacacgtatt actggcagat gaaacggtct gtatcggccc tgctccgtca
    20401 gtaaaaagtt atctgaacat cccggcaatc atcagcgccg ctgaaatcac cggcgcagta
    20461 gcaatccatc cgggttacgg cttcctctcc gagaacgcca attttgccga gcaggttgaa
    20521 cgctccggct ttatcttcat cggcccgaaa gcagaaacca ttcgcctgat gggcgacaaa
    20581 gtatccgcaa tcgcggcgat gaaaaaagca ggcgttcctt gcgtaccggg ttctgacggc
    20641 ccgctgggcg acgatatgga taaaaaccgc gccattgcta aacgcattgg ttatccggtg
    20701 attatcaaag cctccggcgg cggcggtggt cgcggtatgc gcgtagtgcg cagcgacgct
    20761 gaactggcac aatccatctc catgacccgt gcggaagcga aagcagcttt cagcaacgat
    20821 atggtttaca tggagaaata cctggaaaat cctcgccacg tcgagattca ggtactggct
    20881 gacggtcagg gcaacgctat ctatctggcg gaacgtgact gctccatgca gcgccgccac
    20941 cagaaagtgg tcgaagaagc accggctccg ggcattaccc cggaactgcg tcgctacatc
    21001 ggcgaacgtt gcgctaaagc gtgtgttgat atcggctatc gcggtgcagg tactttcgag
    21061 ttcctgttcg aaaacggcga gttctatttc atcgaaatga acacccgtat tcaggtagaa
    21121 cacccggtta ctgaaatgat caccggcgtt gacctgatca aagaacaact gcgtatcgct
    21181 gccggtcaac cgctgtcgat caagcaagaa gaagttcacg ttcgcggcca tgcggtggaa
    21241 tgccgtatca acgccgaaga tccgaacacc ttcctgccaa gtccgggcaa aatcacccgt
    21301 ttccacgcac ctggtggttt tggcgtacgt tgggagtctc atatctacgc gggctacacc
    21361 gtaccgccgt actatgactc aatgatcggt aagctgattt gctacggtga aaaccgtgac
    21421 gtggcgattg ctcgcatgaa gaatgcgctg caggagctga tcatcgacgg tatcaaaacc
    21481 aacgttgatc tgcagatccg catcatgaat gacgaaaact tccagcatgg tggcactaat
    21541 atccactatc tggagaaaaa actcggtctt caggaaaaat aatttgtgac gcgcggtgtt
    21601 tccatcgcgc ttagcaaaga acgtaatcaa cgccgggcct gttaatgcta cccggcgttt
    21661 ttattgccgt taactaacgg tagcctgcaa tttccgcttt caacgcgcta atttcagcat
    21721 cgccatcaac aattcccagc tcaaagcagt atttctttgt ttcccctggc tcaataaagg
    21781 gcagacgccc ctgcgctcgt tctttatcac gaccgtcagg aaaactatta ctgacctcca
    21841 gccccataac atattcaccc gcgcctaaat ttttccactg gataaagttg ggtagctgca
    21901 tggtatcgaa tttctcgtaa attccccacc ccagttcggc gttcaccagc gcaactttac
    21961 tcatcccctg actgtcgcta ttcaacgtga gatagtaaac ctgctcgtca aaaccgggct
    22021 gtggggtaca aatctccaga tgactggcta acccttccgc cgcatgcaga gttcgtggcg
    22081 ttacgctttt agccgacagc aaaatttctg cctgtggcga taataaaggc cagccgtaat
    22141 taaagtgata aagaatttgc agcggctggc gggtgaaacc atgattagtc accttgtctt
    22201 caatgcgcat tacattttcg ccataacggc agcgaatggt cctttccagc gtcagatttt
    22261 caccgaacaa tcgtgtttcc cgtactttgc cattgatgac aaattcaatg ctgtcgtcgg
    22321 tccgttctgt gcgatacccc acctcttctg ctggagtcgc agccaaacgc ccgtgtaacc
    22381 ccagcgtttc tccttcatcc tcacagggcg tgccaatgta gctaagtccg caggtagtca
    22441 gtaaccccgc gtaaaagcta cgtaaaaacc cgttgccctg agactcaaaa aaagcggggg
    22501 cgacaacacc gttgggggtg ataaaagaga gcgcagtgtc cttgtagctg gcccatgcaa
    22561 tatccagcgc gcgatctttt accaccgtaa atgccagtcc cgcaccagta cggacatcaa
    22621 acgcttctgt accctttgct ctgccactac gtaactcata gtgtttaatt ccggcaacct
    22681 gcgagagatc gccaatccac gaccaaattt cgtttttcat tgtgctgctt cccgaatgga
    22741 catgtcatgg cgctggatga acgtttcgat ttccgcaagg cgagacgttc cctccattgg
    22801 gccacgtcgg gttaccgcca gtgcaccgca ggcgttggca tattgtaatg cccgatgggc
    22861 gtcaaaccct agctggcggc aggcaatcca tgcgccacca aaacagtctc ccgcaccggt
    22921 cgggtcaact tcttccaccg gataactctc aacatgaaac tgctcattcg cagagtagta
    22981 actggctcct tgattgccgc gcttcacaat cacctcttta acaccttctt caagaaaacc
    23041 agcgattgca cgctccggcg tcgagtgtgg cgacaagagc aacacttctc cctcactggg
    23101 catatagata tccgttaact caagaacaaa gtggagggcg tcacgcattt ctggaatatc
    23161 gagcatctct ttccgaatat tagggtcaaa agaaataacg ccaccattct cttttacaat
    23221 ggttaccgct ttctttactg cgtctaccat atggaatgaa aacagggatg aacccataat
    23281 atgaaaatgg gtacagtcct tcagaatatt ttcatcaaca tgctgcgccg ataatttacc
    23341 gcaagcggca ttcttgatat taaaaataaa atcacgatcg ccagagttat gataagtaac
    23401 aaaggcactc ccggtagctt ccagaggtaa gactgaaatc cccctgatat ccacaccgtc
    23461 agcagccaga cgatgtatat taatatcacc aaagccatca ttccccacac aactaatgat
    23521 gccgcacgga acgccaagac gtgtcacctg gtcgataaaa atagcgggcg caccactggg
    23581 ataagggccg ttccagatac ctggttgatc aaatggctgg ccgattttac tcgccataat
    23641 ttcaaccaga atttctccaa ttgtgaagac cttactcata tgattaattc cttgaaatga
    23701 ttagctgcgt tttcttttat ttcttacgtc aaaataaacg gctacaagaa tgactaatcc
    23761 tttaaccacc atctggtaaa aataaggaac attcattaag ttcatgccat tattaataac
    23821 gccgatggtt aatgcgccga tcaccgtccc caccacggtt ccatagccgc cgttcaggct
    23881 ggtgcctccc agtacagctg cggcaatagc atccagttcg tagctcaacg cggaattcgg
    23941 ttgcccggaa tagaggcgtg aggcaaggat catcccgttg atacctgcca gcacggctgt
    24001 aatcataaag actttaattt tcaagcgggt gacatttatc cctgaataaa ttgctgcttc
    24061 tttattacca ccagtgatat aaatttttcg tccaaaggtt gttttagtaa gaatgaagtg
    24121 gtttatcagc aataagatga ttaatatcca tataggaata ggtatataga gaaattctcc
    24181 attccccagc gccagaaaag catcatcttt aatcatgatg ggcataccat cggtcactat
    24241 ataagcgata ccacggaaga tccccatagt agcaacggtg acaataaacg aagggatccc
    24301 cgctatagcc gtcaagccac catttatcat ccccagagcc agtgctgcaa taatggataa
    24361 tgggaccaat aaaacaaatg gaattcccca tgaagcccca agcgcagcaa atgtgccaat
    24421 caatgcaatg gttgaaccga ccgataaatc aatatcgcca agcaaaagca cataagtcat
    24481 tccataggcg gtaattgcaa ttatcgaaac ctgaagaata atattagtaa tgttattggc
    24541 ggtaaaaaaa ctgtcattaa taaaactgaa cagaatgatt aacaccacca ttccactgag
    24601 gataccacca tattgcctga agactttttt aaaattattt gcaatgggaa tttccgaagg
    24661 tttcggactg gcaagagttt tctctgtcat ttaacgtact cctgttgcag cggtcattac
    24721 ctgtgtttgc gttaaatttt cacatgaata aatatcggca atcgttttat tacgcattac
    24781 caccagacga tcgctaagtg ccatgacttc tggcagttca gaggacacca gaataattgc
    24841 tgtccctgcc gcagccagtt gacgtatcac gttatagatt tcaaattttg cgccgacatc
    24901 cactccgcgc gttggctcat cgagcaataa aatacgcgga gttttctcca gccacttcga
    24961 taacacgatt ttctgttgat taccgccgct cagtgctccc actgcctgat gcacagaact
    25021 cactttagtg ttcattctgg caattgattg cagcgtcttc gcctcttctt gtcggtgatt
    25081 gatgagtccc atccgcgaag cgttatattg aaacgccaca tggtgcgtgt tcatattgac
    25141 gtcgtgcatc agcactaacc cttcttcctt gcggttttcg gtcacaaaag caatgccatg
    25201 atcaatagcc tgtttgggat tagcgatcct gacttcctta ccatcaatca acacagtccc
    25261 gtgatacgaa acgaggccaa atagcgcctt catcacatcg gaacgcccgg cccccaccag
    25321 gccaaaaaat ccgaggactt cccccggacg cacagcgaaa ctgacctctt taaaaagcga
    25381 aggatgggaa agatttttta cttccagttt tgcaggaaca ttttcatccg gtttttcccc
    25441 aagacgtgct ggccaaatat ttttcatttc ccgaccaacc atttgggcaa tcaagtgctg
    25501 gatatcactg tttttctttt catgcgtagc gatatactca ccgtcgcgga tcaccgaaat
    25561 atcatctgaa atccgcataa tttcatccat gcgatgagag atataaataa ccgcacagcc
    25621 tcgctgcttt aatttttcaa taatgttaaa cagaatctcc gcttcattat cacttagcga
    25681 ggatgtcggt tcatccataa tcacgacatc cgctgcatag cttaaggctt tggcaatttc
    25741 taccagttgc ctatgtgcca tcgacatatt gccgaccatt tccagagggt ctagtgggat
    25801 gcccagttcc gcgaggaggt tctggcaatc tgcgagcatt tttttatcgt taatgatgcc
    25861 aaaacgtcgc ggttcattat tggcataaat attttccgcc acggtcatat tattactcag
    25921 gctcagttcc tgaaaaataa tcgcaatccc taactggcga gaatgctgcg gattattaat
    25981 ggtgatggtt tcaccacgta gtgttatcgt tccctgggtt ggcggataaa caccggccag
    26041 tattttcatt aatgtcgatt taccggcacc attctcgcct aataaagtat gcacccgccc
    26101 tttacgtaaa gagagtgtaa tatcttttaa tgcctgaaca tgaccaaatg tcttcgcaat
    26161 atgcgaaaca tcaagaataa catccgtcat aatgacctcg gtgtcacaac aaaacgccaa
    26221 taacgttttg ttgtgaggtg aagatattta ctatttacct gtataaacgc cgggttgtac
    26281 cgggatcatt ttttctaccg gtttaccatt gaggtgatca acagcggcat caatggcttt
    26341 agcccccatt tgatcgggat attgc
//
LOCUS       DAZFFE010000065        26335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFFE010000065 DAZFFE010000000
VERSION     DAZFFE010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.065"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..179
                     /locus_tag="ACHJ69_002874"
                     /pseudo
     CDS             1..179
                     /locus_tag="ACHJ69_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            513..1097
                     /locus_tag="ACHJ69_002875"
     CDS             513..1097
                     /locus_tag="ACHJ69_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO5015824.1"
                     /translation="MITITKGRLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AKPIGAFHIAEQQVDGTSDYLKDGEWPIDNGIIEVYAAPPIPVVPEEKPMPNPLKMYA
                     VDAVAAIAEVRGWNACRAAMLQSQGNCIKDGWISCSEQMPDKLIPVMVMYEDGEMWSA
                     MWNGNRWDDGTEYPDPHSVTHWREMPAAPKQEVK"
     gene            1097..1219
                     /locus_tag="ACHJ69_002876"
     CDS             1097..1219
                     /locus_tag="ACHJ69_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015825.1"
                     /translation="MNKCNALLYAMVIGFGLAAGIRVYIAWESLINLAWSAIRG"
     gene            1212..1550
                     /locus_tag="ACHJ69_002877"
     CDS             1212..1550
                     /locus_tag="ACHJ69_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015826.1"
                     /translation="MAKSPAERKAAQRARQSAAGERKIELVLDKQEQEMLARNCAARR
                     PGRDPYEMAEYIALLIRQDDARVRGRIKSISRKRCGKCGERVPVNSCPCNGDSQCWVT
                     KGWHETKLIV"
     gene            1591..2265
                     /locus_tag="ACHJ69_002878"
     CDS             1591..2265
                     /locus_tag="ACHJ69_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5015827.1"
                     /translation="MNTAANYGGFILHGTITTTVTITTVVMMPAEHKTYKRKSTQFKP
                     LTAMQEAYCQSYIKTPENQTQAAINAGFSPNTAAVKASVMMRDERIQKRIAELMEERN
                     KRMRVSADYVLMRLVEIDQMDVIDILNDDGSLKPIREWPKIWRTTLSGFDLSSTIMNM
                     NEDSIETILKKIKWPDKVKNLEMIGKHVDVNAFKERLDVNVNVTIADRIAAARKRLKE
                     RQDGNQ"
     gene            2262..3737
                     /locus_tag="ACHJ69_002879"
     CDS             2262..3737
                     /locus_tag="ACHJ69_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO5015828.1"
                     /translation="MTDTALSPEEQLIEDIAGFTHDPLGYALYAFPWGEEGTELAHAT
                     GPRKWQADAFREIRDHLQNPETRYQPLMLARASGHGIGKSAFISMLINWGMSTCEDCK
                     VVVTANTDNQLRTKTWPEIIKWSNLAITKYWFTCTATAMYSNDPGHDKRWRADAIPWS
                     EHNTEAFAGLHNERKRIIVVFDEASNIADLVWEVAEGALTDEDTEIIWVAFGNPTRNT
                     GRFRECFRKYKHRWKTAQIDSRTVEGTNKQQLQKWVDDYGEDSDFVKIRVRGIFPDAS
                     ELQFIPTGLTDEAMKRVVTAAQVAHAPVIIGVDPAYSGVDDAVIYLRQGLHSKVLWTG
                     NKTTDDLIMAKRIADFEDQYQADAVFIDFGYGTGLKSIGDGWGRTWQLVPFGGASTDP
                     QMLNKRGEMFNACKTWLRLGGMLDDQETADDLSAAEYKVRVDGKIVIEPKEDIKERLG
                     RSPGKGDALLLTFAFPVSKRLRIPGQQNQQGKAITDYDPYA"
     gene            complement(3828..4199)
                     /locus_tag="ACHJ69_002880"
     CDS             complement(3828..4199)
                     /locus_tag="ACHJ69_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp49 family protein"
                     /protein_id="HFO5015829.1"
                     /translation="MNDQQIEKEIVEKGKTAPRVTPDHIGSIIAQEAYFTAEDGAFGK
                     AIKAKHTGGEVNYQPHESLSLLTFCVLVLRNGFTVTGESSCASPENFDAEIGRKIARQ
                     NAVNKIWMLEGYLLKQKLSEQ"
     gene            complement(4398..4474)
                     /locus_tag="ACHJ69_002881"
     tRNA            complement(4398..4474)
                     /locus_tag="ACHJ69_002881"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4438..4440),aa:Arg,seq:tct)
     gene            4902..5108
                     /locus_tag="ACHJ69_002882"
     CDS             4902..5108
                     /locus_tag="ACHJ69_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015830.1"
                     /translation="MCMGSSPSVPATPEVQAAPQEQDAAVVDARDEETRRRRAAAGRS
                     STLLTGSQGDTSTANTSGKTLLGQ"
     gene            5123..6801
                     /locus_tag="ACHJ69_002883"
                     /pseudo
     CDS             5123..6801
                     /locus_tag="ACHJ69_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
     gene            6798..7094
                     /locus_tag="ACHJ69_002884"
     CDS             6798..7094
                     /locus_tag="ACHJ69_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015831.1"
                     /translation="MTDYEDDQLKEENARKQRDMAQREIDDIRFVMSSEQGRRVVWSV
                     LEKGRVFSAISPMDAMAMAFNEGQRNLALELFQRVMAHCPEQYLKMAKEASEQE"
     gene            7097..7792
                     /locus_tag="ACHJ69_002885"
     CDS             7097..7792
                     /locus_tag="ACHJ69_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFO5015832.1"
                     /translation="MNLFERLLYRRLCNEQPVDGGAAPAASEPSAPAGDNPAPVGDPS
                     QQEGDKPQPVADGDKPADDKKPENDKQDEKKDGNKPEGAPEKYEFQAAEGVELDTEAL
                     KEFEPVARELNLTNEQAQKLVDAYPKILAGVQQRQAEAWQKTTEQWAADVKADKEIGG
                     DKLISNLSAAQRALDQFGTPELKEYLNTTGLGNHPDLVKTFVKIGKAMSEDGMVTGGN
                     EGQRSAAEVLYGK"
     gene            7807..8793
                     /locus_tag="ACHJ69_002886"
     CDS             7807..8793
                     /locus_tag="ACHJ69_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO5015833.1"
                     /translation="MAVKGLTALTLADWGKRVDPNGKVDKIIELLGQTNPILQDMPFV
                     EGNLPTGHRTTIRSGLPSATWRLLNYGVQPSKSTTVQVTDSVGMLETYAEVDKSLADL
                     NGNTAEFRLSEDRAFIEAMNQQMAQTLFYGDSSVNPQQFMGLSSRYSSLSAGNAQNII
                     DAGGTGTDNTSIWLVVWGENTVHGIFPKGQKAGIQMEDKGQVTLEDVNGGKYEGYRTH
                     YKWDNGLALRDWRYVVRIANIDVSNLSEPSSAANIAKLMVKALHRIPNRGMGRPVFYM
                     NRTVGQALDLQSLEKTSLAISVKETEGEWWTSFRGVPIRETDALLETEARVV"
     gene            8845..9282
                     /locus_tag="ACHJ69_002887"
     CDS             8845..9282
                     /locus_tag="ACHJ69_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bbp16 family capsid cement protein"
                     /protein_id="HFO5015834.1"
                     /translation="MITDKLLMFSEAQAVTNTAASTDVIDLGPIDGKRRDIGVGYPLE
                     FWALVNTAAAASGDATVNIQLQTSENNSSWTTIYDSGALAKTALTAGKRVVSAKVPAG
                     VQRYLRVNYSVATGPLTAGEFTAGISLDVDANTPYPIRSKVTG"
     gene            9293..9628
                     /locus_tag="ACHJ69_002888"
     CDS             9293..9628
                     /locus_tag="ACHJ69_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015835.1"
                     /translation="MSGEKPRYRVLRLSHIHNTLWPEGAEIEYEGEPGSALEPVNDAA
                     RQAKAKVAGKVSMAATSTKIINDVSDDGELDKLREEYELLFNEKPHHNAKAETLREKI
                     ADKRKELGV"
     gene            9679..10002
                     /gene="gp10"
                     /locus_tag="ACHJ69_002889"
     CDS             9679..10002
                     /gene="gp10"
                     /locus_tag="ACHJ69_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid staple protein"
                     /protein_id="HFO5015836.1"
                     /translation="MELVNLKTGTDSYQDESGETRTRDEYPWGLCITLNNDTLNKLKA
                     QPQGVGTEVMITAKAVIRGLSARETDDGVNRSADLQITDMAIAPVSGDVEKSAAETLY
                     GNGGE"
     gene            10002..10607
                     /locus_tag="ACHJ69_002890"
     CDS             10002..10607
                     /locus_tag="ACHJ69_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015837.1"
                     /translation="MASVVEICNRALSNIGNSRSINSLTEASKEAGECSLHFEACRDA
                     VLSDFDWNFATKRVALADTSNPPPDWEYAYQYPSDCLRITEIMLPGVRNPTAAMRVQY
                     EVGADTNGTGKLIYTDQPQAWLKYVSRVSDVNMFDAIFMEALAWRLAAAINMALTGNA
                     DLGTFALNMYNREILSAGSHSQNESQEPQPPVDEFTIARLS"
     gene            10607..13078
                     /locus_tag="ACHJ69_002891"
     CDS             10607..13078
                     /locus_tag="ACHJ69_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015838.1"
                     /translation="MAISWIQPSFAGGEIGPSLYGRIDMAKYQVALRKCDNFIVRQYG
                     GVENRPGTRFVGAAKYPNRKCRLIPFQFSTVQTYALEFGHQYMRVIKDGALVLNSSNV
                     IYEIATPYTEADLFRIKFTQSADVLTLVHPAYPPKELRRYAHDNWQLVDVVTKNGPFE
                     DINIDESVTVYASASTGTITLTASASIFGVEQVGKLFYLEQPAVDSVPVWETSKSTSI
                     GDIRRADSNYYRAVTAGKTCTLRPSHTEGTSWDGWGGSGDDDTGIEWEYLHSGFGIAR
                     ITAANGTTATAEVISYIPSQVVGANNASYKWAKYAWNSVNGYPGTVVYYQQRLYFAAS
                     TAFPQTIWASRTGDYKDFGKSNPTQDDDRIIYTYAGRQVNEIRHLIDVGSLVALTSGG
                     EYVITGDQNKVLTPSSFAFSSQGSNGSSNVPPIAVANIALFVQEKGSVVRDLAYSFDV
                     DGYQGNDLTILANHLFQKHSIVDWCFSIVPYSSAFCIRDDGKLLVMTYLRDQQVFAWA
                     PQSSTGKYESTCSISEGNEDAVYFVVNRTVNGQTVRYIERLSSRLFTSDEDAFFVDSG
                     LSYDGRNTSDRTMTITGGSGEWDYRTEYTISVSGGAYFTSSDVDAQLQFPYTGTDPDT
                     GDEVSKELRCDIISVTSNTAVVVRANRNVPPSLRNAATTNWQMARRTFGGLSHLEGQT
                     VNILSDANVEPQKVVSGGAVTLESPGAVVHIGLPITSEFETLDININGQETLLDKKQV
                     IPSVTLVVNASRGIWATTPGGKWYEYPQREFEFYDDPVDDATGKVDVKLDSNWGKNGR
                     VRIRQLDPLPLSVLAVIPRLTVGGF"
     gene            13078..13542
                     /locus_tag="ACHJ69_002892"
     CDS             13078..13542
                     /locus_tag="ACHJ69_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015839.1"
                     /translation="MIDVRIIPATEEHLQMILPDVRQADIDELYAVSLMTTEDALRVG
                     LRTATMAWSGFANRELVTMFGVSPASMIGGNGTPWLVGTSRIEKYQKTFLRHCRPVLQ
                     QMLAVYPRLENYVDERNHVAKAWLHWLGFRLEEAAPYGALGLNFHRFHMERK"
     gene            13542..14087
                     /locus_tag="ACHJ69_002893"
     CDS             13542..14087
                     /locus_tag="ACHJ69_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015840.1"
                     /translation="MCDPVIAGGAMLAMSGIQAYTQYQQGKYASKVAEANADIATAQA
                     NDAINRGNAEAEQRRRETRQRLGTQAATMGATGADLSTGNALDIFGDTAQFGALDALT
                     TVNNAQREAYGYQVQAANYKAEASSARKQGNVGAATTLLTAPLKAYGAYQMFGGTWSP
                     FTQSTHAPIGAAAGTRLPGGL"
     gene            14087..16600
                     /locus_tag="ACHJ69_002894"
     CDS             14087..16600
                     /locus_tag="ACHJ69_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015841.1"
                     /translation="MPVVPTVSGRQVESRGVQSAGLQTFSQPGIGDAFVRAGTEAIDV
                     LGQAKQRANIALAQEASLNLSQISSDLLNNPETGLLNLKGKNATGKGQEYTQQFDAQV
                     EQLAMSLPDEQARNAFMQQAQQQRIQFTTQAGRHEIGQINAYEEGQFQATLLNNGKNA
                     AALYGDNAAYVLANKQTFQQIEDYGIAHGWSDEQIQAKKIEFKEKVADAALSQWSASN
                     ATAFIQSNGELSDTAAGARRAVADNDSSERARGIRNNNPGNLEYSKTNPWVGQTGDDG
                     RFAKFETPEHGIRALGRNLMSYQRQGIDTVSEIINRWAPPTDKNDTMSYIKAVCEQLG
                     VSADEPLDASNPDTLKALCAAIIHHENGSQPYSDQQLTAGVSAALGLSTIPTNTKRYT
                     GNAAFDAASPEAQASFMRQADQLRRQQQAEYKTMIDSQVRDATAAYMRGVEFPNPPGE
                     DDFIAAYGVREGNLRYTEFKNTQIAGQYIGSFRNMPTSSITAYVEQLRPDTGETGEGY
                     AARAALYDNVVSAANQVIKQRQSDPVQFSLAAGQAKPIDMSNKDNFGQSVALRAAQVS
                     DLAKSYGTPLTFFSKDEANQIGVFFRDAPVSQQAAYLDTIRQSTGGGQVYMSALQQIS
                     ANAPSAAVAGILMDKPGGILAEKNWFNPDVSVSPETAAQTILAGAAARKGTDDAKGIP
                     MPKDADLRLEFSDMVKDAFAGDAQGASMAYEIAKDYYAGVMAKKGVVSGEIDNDIWKQ
                     AVNVATGGVHDYNGMGNVLLPWGMSAEQFDKQVNQAWNEQVVGTGIKTPPGQYGLQSY
                     GDSQYLVKLGTGYLLKDDGSPVVLDLTQKRQRFSGDIPQ"
     gene            16597..18399
                     /locus_tag="ACHJ69_002895"
     CDS             16597..18399
                     /locus_tag="ACHJ69_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015842.1"
                     /translation="MSYFGLNPVNQNQQLDEAASNPAGFNSDVGFFDNAVGAALSGLY
                     SGLVAKPDQLLWAGMDKIVSPIAQFVNENTSLNDTSVSYIAEQRKLAEQQVKRLTPDA
                     ATTGTAGQVLYGLFDMGGQAVVGTTLGGPVGGAAAVTSLQGFSEFERLTAQGVDFRTA
                     QEAGLVQGITAGAGTLIPMSLGLRAGGALAEGVAAQLARTGESSVRRAAATAVRATPD
                     IAYAAGTNIAFGMAQRGLTAKTLRDGGYSEMANQYDVLDRQAIAIDAVLGVAFGGVGR
                     FINSRGESTNAPNFSPVDIDAALAANAAHHAEIDIAPGVPINVLSRNSHIQALRKAMS
                     AVSQGRPVDVASIVESASFSEIPGRKNLLSQAVNEALSSVDDGVTARAIENRLLEEQA
                     AQLLPRGDRQVYQSEIANSQRILDNLTEQRAQILAEEPTGSGKALSRARSDKQARIRD
                     IDQRIRQAQERLEFSRNALAPHEHGGQFFEARAELARRLQVESELNAQAVSFYKTAEV
                     RTPDEVAPFEPDKILQQAEQKMMADQAGDIDLRIAEDSLLESPDMIITVLDDDGNPQS
                     RSAREVLDEANRESEQAIQDSSLFDVAVACFLRG"
     gene            18405..20879
                     /locus_tag="ACHJ69_002896"
     CDS             18405..20879
                     /locus_tag="ACHJ69_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015843.1"
                     /translation="MRQECIQAVQQAAQRTLTAREIQNIEDRIYRNMRSIARDDPMSW
                     RQLSESERLYRAAQLASEELQREAALKKRRVALTIAARQRLDKFINSYQGADGKLGAL
                     NRTIAFNADGKSNFLSVESRTKATRDYALSQLQEAFEAVDPRFFGLFEDEAGVRDLVY
                     EMRGQNTGNAKARKGAKAWREVTDLLRRRFNDAGGDIGYLENWGIPQHHSMEKVGAVS
                     KDKWVSDVIGKLDRKYYIRADGQLMNDAELSAFLGEAYNTIATGGLNKLTDTGMRISG
                     ARANRGNASRQIHFKDADSYLQYQQLYGDRSLWEIMVGHLEGISKDIALVETYGPNPD
                     HVFRSLLDQVKAETATANPSKTGSVERLANKTENLYNFISGKTQPVANPHIARWSDNI
                     RNWLVASRLGSALLSSFSDLGTIYLSAKVTNLPMNQLFRNQLEAMDPTNRTELARARR
                     AGLAMESLLGSVNRWAMDNMGPSVSRWAATAVMRASGLTAWSDAHKRAYGVTMMGSLG
                     EVVSRTPDLRSLDDSDFRILKSKGITDTDWSVWKLAQQEDWGNGNNTMLTPESIMRIP
                     DSAVKHLGEPERVKFEAMRKLLGAVTEEVDMAVITPGAREQMFVGSGLQRGTWKGELT
                     RSVFLFKSFPISVVMRHWHRAMGMPSAGGRAAYIATFLASTTMLGALSMQITDLINGR
                     NPKEMTGDNMVKFWINAFLKGGGAGLYGDFLFSDHTRYGSGALASMLGPVAGLVDDVV
                     KIAQGIPLNAVEGKNEQTGGDLVKLGKGLMPGANLWYLKAALDHMIFNQMQEYFSPGY
                     LRKMEQRSKKEFNQTYWWRPQDVTPQ"
     gene            20889..21302
                     /locus_tag="ACHJ69_002897"
     CDS             20889..21302
                     /locus_tag="ACHJ69_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015844.1"
                     /translation="MKELLLLSVFLISACSTSYDVYDGVDKAYCDKVKMDFSLAKTAK
                     DSCIDHYVKTYTKPASSASDIAEGAVFECNKVISIAASSSYDAAVCAMAERNGMSVQK
                     INSMISSNDEAKIRTDISSVKKDAMNRVVKYQSSL"
     gene            complement(21341..21502)
                     /locus_tag="ACHJ69_002898"
     CDS             complement(21341..21502)
                     /locus_tag="ACHJ69_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane anchored protein"
                     /protein_id="HFO5015845.1"
                     /translation="MKKAFAALFVLLSLVASTQAFAGRCQHDSDTAADGSRCGGRSAD
                     SRPGGGGIR"
     gene            complement(21595..22140)
                     /locus_tag="ACHJ69_002899"
     CDS             complement(21595..22140)
                     /locus_tag="ACHJ69_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafO family
                     toxin"
                     /protein_id="HFO5015846.1"
                     /translation="MSSSASQNDKNQIVRYKGRVLHTQNFSALCASDLELKKVSDAFT
                     QYWKTGYHPSLGKDAAFARPTEMLKLNVRHTHVDNQDYIPEDSDKKHTGKKSSWDAWK
                     NIASVQVKCIPTSDCFLVYSVNHNRDALVMFFVDADAHNITEQEEFKEAAITISYQFF
                     EKTKTEPMPLEEDLFSDKWKE"
     gene            complement(22442..23152)
                     /locus_tag="ACHJ69_002900"
     CDS             complement(22442..23152)
                     /locus_tag="ACHJ69_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFO5015847.1"
                     /translation="MANAQTAIFKFESVNPIRSIIIDGQPWFVAQDVCSALRIQNVTQ
                     SLEKLDDDERSMFNIGHEHRAIFDSRVKEINIISESGLYTLILRCRDAVTPGTIPYRF
                     RKWVTGEVLPQIRRTGSYIKNSLPQEERIKMVADQVANATASAVIQAMKIENKTYSAP
                     LKPGYRSLIHSPSGVLGLTENSLLMNLLNQLQEDGHDVSGAAAELTTMFCYIVGVSKC
                     LRDIQTHAEYINDKAGFF"
     gene            complement(23468..23725)
                     /locus_tag="ACHJ69_002901"
     CDS             complement(23468..23725)
                     /locus_tag="ACHJ69_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015848.1"
                     /translation="MQQYHYPLEDGFTERIHTPGGVRLLVEGSLLMKLLRDLDKDGFN
                     VDGPLAELTALINYVTSSQMSMQDLQTHLDYCAEQLRKQTR"
     gene            23921..26023
                     /locus_tag="ACHJ69_002902"
     CDS             23921..26023
                     /locus_tag="ACHJ69_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015849.1"
                     /translation="MTVSTEVDHNEYTGNGVTTSFPYTFRIFQKSDLVVQVVDLDENI
                     TVLALDTDYTVTGAGGYNGGNVILSKVLANGYLISIYRELPVTQETDLRNQGKFFAEV
                     HENAFDKLTMLIQQVRNWFSLALRKPSFVANYYDAMNNYIRNLRDPSRPQDAATKNYV
                     DGLAETNLSRTLRTPEPISTLPGVEQRKNKIVAMDDSGDPIMVLPESGSATDVMLQLA
                     ATDGFKNIGSCPDVATLRNTEPSSVNQQIIVRGYYSDTPGLGGGVFMSFDSSEADDGV
                     NIFVTPGGKRWKRAGSHIDIPVENGGMMTSCTAEQNSEAFERLTACLPYEGGTLRLNG
                     FYDIKYGAIVPPRVTLEGCGMDSCGLIKTGNDIKTVPDRMWQGVPHSFSKDFIVAVDM
                     DSDTSGDLSGKQTRSTRIIGLSILNTAPNPCDYGIYSAISYNVRLQDLYIHRVKTGYR
                     TSDSWLQTWENITIQDVVTGVKIEAGGTTYNLNNIYVKTCSGVAYDFNNITYSSLTCL
                     AADFVNGTGYKFKDCTGVNLSGCGSEEGTGKIFDINGSRINVTAFRGVNHHDGGVEAV
                     SLESCFISLVSSHFSGYSGVSSSKYIYLNDATANFNGAVIPDNTTDSIRWGSGQSWIN
                     KVDSGGTYSITGNSSWTALCRLESGYITVLSVSPPTGDTTNFPFGTKWELLGPVSTGP
                     WKWTYVSTGEWKVSATTT"
BASE COUNT         6903 a         5970 c         6976 g         6486 t
ORIGIN      
        1 atggcgttaa cacaccacga actctgtcag attgcgtaca agttccttaa gcgcaacggg
       61 ttcaaggttt gctttcatga ccgctttgtt gctgtaacca gtaccggaga acagccagat
      121 gctatgggat tcagaaattc agcatcatgc ctgatagagg cgaagtgttc tcgtgctgac
      181 ttgttggcag atagaaaaaa gcgtttccgt aaaaatccct cacttggcat gggcgactgg
      241 cgattcttta ttagtgagcc ggaaattatt tcagttgagg atttacctcc cggctgggga
      301 ttacttcacg ttgttaacgg aagagtacgg aaagtacatg gatggcccag gggtaattgc
      361 tgttggggta atcctgacga taagccattt accgggaata agcaggttga atgcgattac
      421 atgttatctg cattaaggcg catggagttg agagggcacc ttaatgaaat atatgacggt
      481 gtgattgtta ataagaaaga aggaaacgcg gcatgatcac tattaccaaa gggcgactgc
      541 tgacaatcaa gcagtggcgc gaaacatacg gaccgggtag caacgttgta ctgccagcag
      601 aagaagcgga agaactggca cggattgcac tggcatcatt ggaagcaaaa ccaataggtg
      661 cattccacat tgcagaacag caagttgacg gcacaagtga ctacctcaag gatggagaat
      721 ggcctattga taatggaatt attgaggtct acgccgcccc acccattcca gtagtacctg
      781 aagaaaaacc aatgcctaac cctcttaaaa tgtacgcggt cgatgctgtt gccgctattg
      841 cagaggtgag aggctggaac gcctgtcgtg ccgctatgct tcagagtcaa ggtaattgca
      901 ttaaggatgg ttggataagc tgtagtgagc agatgccaga taagttaatt ccggtaatgg
      961 tcatgtatga agacggtgag atgtggtctg caatgtggaa tggcaatcgc tgggatgatg
     1021 gcaccgaata tccggatccg cactcagtta cgcactggcg tgaaatgcca gcagcgccga
     1081 aacaggaggt gaagtaatga acaagtgcaa cgctctgctt tatgccatgg tgattggttt
     1141 cggcctggct gctggtatcc gggtttatat tgcctgggag tcattaatca atctggcgtg
     1201 gagtgcgatt cgtggctaaa tcccccgcag aacgcaaagc cgcgcagcgc gctcggcagt
     1261 ccgccgccgg tgagcgcaaa attgaactgg tgctggataa gcaggagcag gaaatgctgg
     1321 cgcggaactg cgccgcccgg cgccctggtc gcgatcccta tgaaatggcc gagtacatcg
     1381 cgctgctgat ccgccaggat gatgcacgcg tgcgcgggcg tataaaatcg atcagcagaa
     1441 aacgttgcgg taagtgcggc gagagagttc ctgtgaattc atgcccgtgt aatggtgact
     1501 cgcaatgctg ggtgaccaaa ggctggcatg aaacgaaatt aatagtgtga catgtcacga
     1561 gtagattatg catgatgaat ttgatgggtt ttgaatactg ccgccaacta tggcggcttt
     1621 attttgcatg gtactattac cacaacggta actattacca cggtggttat gatgcctgct
     1681 gaacataaaa cctataaacg caaatcaacg caatttaagc cactaacagc aatgcaggag
     1741 gcttattgcc agtcatacat caaaacgcct gaaaaccaga ctcaggcagc gattaacgca
     1801 ggattctccc caaatacagc ggcagttaaa gccagtgtca tgatgcgcga tgaacgcatt
     1861 caaaaacgga ttgccgagtt gatggaggag cgcaacaaac gaatgcgcgt cagtgctgat
     1921 tacgttctca tgcgcctggt ggagatcgac cagatggacg tgatcgacat cctcaacgac
     1981 gatgggagcc ttaaaccaat ccgtgagtgg ccgaaaatct ggcgcactac gcttagtggc
     2041 tttgatctgt catcgaccat catgaacatg aacgaggatt cgatagagac aatcctcaaa
     2101 aaaattaaat ggcctgacaa ggtgaagaac cttgagatga ttggtaagca tgttgatgtc
     2161 aacgcgttca aagaacgtct ggatgttaat gtgaatgtga caattgctga tcgcatagca
     2221 gcagccagga agcgactcaa agaacgtcag gatggtaatc agtgacagat acagcgttat
     2281 ctcctgaaga gcagttgatc gaggatattg cagggttcac tcacgatccg cttggctatg
     2341 ccctctatgc gttcccgtgg ggggaagagg ggactgaatt agcacatgcc accggtccac
     2401 gtaagtggca ggctgatgcg ttccgagaga tacgtgatca cctgcagaat ccagagacgc
     2461 gctatcagcc gcttatgctg gcacgtgctt cgggtcacgg tattggtaaa tccgcattca
     2521 tctcaatgct gatcaactgg ggcatgtcca cttgcgagga ttgtaaggtc gtggtgaccg
     2581 ccaacaccga caaccagcta cgaacgaaga cctggccgga aattatcaag tggtcgaacc
     2641 ttgctatcac gaaatactgg tttacctgta ctgctaccgc gatgtacagc aatgaccctg
     2701 ggcatgacaa gcggtggcga gctgacgcaa tcccctggtc tgagcacaac actgaggcat
     2761 tcgccggact acacaacgag cgcaaacgca tcatcgtggt attcgatgaa gcgtcgaaca
     2821 ttgccgatct ggtgtgggag gtagcagagg gtgcgctgac ggacgaagac accgaaatca
     2881 tctgggtggc gttcggaaac cctacacgta acaccgggcg ttttcgcgaa tgtttccgca
     2941 aatataaaca ccgctggaaa actgcgcaga ttgacagccg gacggtggaa ggcactaaca
     3001 aacagcagtt gcagaaatgg gttgatgact acggggaaga cagcgacttc gttaaaatcc
     3061 gtgtgcgcgg catattcccg gatgcatctg aattgcagtt tatccctacc ggccttactg
     3121 acgaggcaat gaaacgggtg gtaaccgctg cgcaggttgc acatgctccg gtgataatcg
     3181 gcgttgaccc ggcatactcc ggcgttgatg acgctgtgat atacctgcgg caggggctgc
     3241 acagtaaggt gctatggact ggtaacaaga ccaccgacga tctgattatg gcgaagcgta
     3301 tcgctgactt tgaagaccag tatcaggctg acgcagtgtt catcgacttt ggttacggta
     3361 ccggtctgaa gtcaatcggt gacggctggg gtcgtacatg gcaacttgtt ccgttcggcg
     3421 gtgcgtccac tgacccgcag atgcttaaca agcgtgggga gatgttcaat gcatgcaaga
     3481 catggctgag gctcggcggc atgctggatg accaggaaac agcggacgac ctgtcggcgg
     3541 cagagtacaa agttcgagtg gacggtaaaa tcgttatcga accgaaggaa gatatcaaag
     3601 agcggcttgg gcgttctcct ggtaaaggcg atgcgctact gctgacgttt gcgttccctg
     3661 tgtcgaagcg tctgcgaatt cccgggcagc agaaccagca aggcaaggcc atcacagatt
     3721 acgatcccta tgcttaatcc tctggtgggg ataatgtcgt tgatatcctc tggtgaggat
     3781 aaaacaaagc cagctcatag gctggctgtt tgtgacatgt cacggtgtta ttgctcgctt
     3841 aacttctgct tcagcaagta accttcaagc atccagattt tgtttacagc attctgtcgg
     3901 gcaatcttcc gaccaatttc tgcatcaaag ttttccgggc ttgcacagga actctctccg
     3961 gtgacggtga agccgttgcg cagcaccagg acgcagaacg tcagcagaga aagtgattcg
     4021 tgcggctggt agtttacctc tccgccagta tgtttcgctt ttatggcttt gccaaaggca
     4081 ccatcctctg ctgtgaaata tgcctcctga gcaataatgc ttccgatatg gtctggcgta
     4141 acgcgcggag cagttttgcc tttctcaacg atttcttttt cgatttgctg gtcgttcata
     4201 atctcacctt aaaaaaatgc ccggcgaacc gggcgaactg gaagcaatga gttatgcctt
     4261 ccgtggctgt acgggtttac agcatgaagt catcgcaatg gcgtcctgct gtaaaaaggg
     4321 cggtgatagc ccttcaaggg aaaccatcac cgccaagccc ctggaacttc tggcatcacg
     4381 gtccttaggc gtgattctgg cgtggcatgc aggattcgaa cctgcgacca accgcttaga
     4441 aggcggttgc tctgtccaac tgagctaatg ccacaacgct gagagcactt agcctgttaa
     4501 ggcgccacac tttgtcgcgg ctccataaat gctctcatcg ttgtaccctc gtctcttccg
     4561 aggcgtcaca ccgaatcgcc gggatggtga atccccgtgc gcggaataaa accgctcgac
     4621 ttgcacattc cggctacctg gttcgtttgc tcgagcaagg gagggtgccc cttaaacgta
     4681 tccagaccgc tatcggcgca tgtgccatac gccgtactgc tcaaaataaa agctcactcc
     4741 acctgttcaa tttaacgaca agccagtcag gttaataacc ggaatgaact ctttgcttac
     4801 ctgaaaggta ataattcgtg cgttaaatgt caactatcta cgataaataa atcatatgtg
     4861 gttaaattgg taataattta attgcgtacg gagtcattga tatgtgcatg ggtagctcac
     4921 catcagtgcc tgcaacacca gaagttcagg cagcaccaca ggagcaggat gccgccgttg
     4981 ttgatgcccg cgacgaagaa acacgtcgcc gtcgcgctgc tgctggtcgt agttctacgc
     5041 tgcttaccgg ttctcagggc gacacatcaa ccgctaatac cagcggtaaa acgctgcttg
     5101 gtcagtaacc ggagtcattg aaatggcgga aacaactaaa gagcgattga acaaacagtt
     5161 cgcacaactt gaaagcgagc gtcagtcgtt cgagccgcac tggcgcgagt tgagtgatta
     5221 catcaacccg cgtggttccc gctttctgac ttctgaggtc aaccgtaacg atcgacgcaa
     5281 tacacgcatt attgattcga ccgggactat ggcggcgcgc actctcgcca gcggcatgat
     5341 gtcaggcatc acaagccccg cgcgtccgtg gtttcgcctg gctacgccag atcctgaaat
     5401 gatggattat ggccctgtta agttgtggct tgaggggtgc agaaccgcat gaacgatatg
     5461 ttcaataagt cgaatctcta tcagtcgctg ccgcagttat acggaagcct cggcacatac
     5521 agcaccggtg caatggcggt actggaggat gacgaggaca tcattcgcac aatgccattc
     5581 ccgataggca gttactacct ggctaactca cctcgtggca gtgtggacac ctgttttcgc
     5641 aagttctcta tgactgttcg tcagcttgtt caggagttcg ggctaaataa cgtcagcgaa
     5701 tccgtaaaaa gcatgtggga aagcggtacc tacgagaagt ggattgaagt gatgcattcg
     5761 gtttacccga acattgaccg cgatacatcg aagctggata gcaagaacaa gccattcaaa
     5821 tcggtttatt acgaggttgg tggcgataac gacaagttgt tgcgtgagtc aggattcgat
     5881 gagtttccaa ttatggctcc gcgctgggaa gttaacggcg aagatgttta tggatcatca
     5941 tgcccgggta tgctggcgct tggacctgtt aaggcattgc agcttctcca gaagcgcaag
     6001 tcgcagttga ttgataaagc caccaatccg ccgatggttg ctccgacttc cctcaagaat
     6061 cagcgcgcct cccttcttcc tggcgacatc acgtatatcg atcagattac tggtcaggat
     6121 ggcttcaggc ctgcttatct ggttaacccc agtacagcag atctggtagc agacattcag
     6181 gacactcggc aaatcattaa cagcgcctac tttgtcgatc tgttcatgat gttgcagaac
     6241 atcaataccc gctcgatgcc tgttgaagcg gtgatcgaaa tgaaagaaga aaaacttctg
     6301 atgttggggc cggttctgga gcgtctgaac gacgaatgtc ttaatcctct cattgaccgc
     6361 gctttctcga tgatggtgcg taaaaacatg ttgccgccac cgcctgacgc gatggaaggc
     6421 atgcccctga aggtcgaata catttccgtc atggctcagg ctcagaagtc tatcggcctg
     6481 tccagtctgg cgtccacggt taacttcatt ggtcaacttg cgcaagcgaa accagaagct
     6541 ctcgacaaac tcaacgttga tcaggcgatc gatgcattcg ctgatatgtc cggagtgtct
     6601 ccaaccgtca ttgttccgca ggaacaggtt gagcaggctc gccagcaacg ggcacagcag
     6661 caacagcagc aacaaatgat ggcgatgggg atggcggcgg cacagggtgc caagacgcta
     6721 agcgaagcta aaacttcgga tccgagtgtt ttgtcagcta tggcgaatgc agttagtggt
     6781 cagggtgggc aatcacaatg acagattacg aagacgatca actgaaagaa gaaaacgccc
     6841 gtaagcaacg tgacatggcg cagcgtgaaa ttgatgacat tcgctttgtc atgagcagtg
     6901 aacaggggcg tcgcgttgtc tggtcggtgc tggagaaagg ccgtgtgttt tccgctatct
     6961 caccgatgga cgctatggca atggcattta atgaggggca acgcaatctg gcgctggaac
     7021 tgtttcagcg cgttatggcg cattgccctg aacagtattt gaagatggcc aaagaggcca
     7081 gtgaacagga gtgatcatga atttatttga gcgtttgctg tatcgccgtc tttgcaatga
     7141 gcaaccagtc gatggtggag cagctccggc tgcgtcagaa ccgtcagcgc ctgcaggtga
     7201 taaccctgct ccagttggtg atccatcaca acaggaaggt gataagccac aacctgttgc
     7261 tgatggcgat aaacctgctg atgacaaaaa gcctgaaaac gataagcagg atgaaaaaaa
     7321 ggacggcaat aaaccagagg gtgcgcctga gaagtacgag tttcaggctg ccgaaggcgt
     7381 agagctggat acagaagcgt tgaaggaatt cgagccggtg gcgcgagaac ttaacctgac
     7441 caacgagcaa gcgcaaaagc tggttgatgc ttatccgaag attctggcag gtgttcagca
     7501 gcgccaggca gaagcctggc agaaaacaac cgagcagtgg gctgcggatg taaaagctga
     7561 caaagaaatc ggtggcgaca agttgatttc taaccttagc gccgcacagc gtgcgcttga
     7621 ccagttcggg acacctgagc tcaaagaata tctgaacacc accgggctgg gtaatcaccc
     7681 tgatctggtc aaaacgttcg tgaaaatcgg aaaggcgatg tctgaagatg gcatggtcac
     7741 cggtggtaat gaaggccagc gtagtgcggc cgaagtgctc tatggcaaat aagagaggaa
     7801 atgacaatgg ctgttaaagg cttaactgcg ctaacgctgg ctgactgggg taagcgcgtc
     7861 gatccaaacg ggaaagtcga taagattatc gagcttctcg gtcaaactaa cccgatcctt
     7921 caggatatgc cttttgtcga agggaacctt cctaccggac accgaaccac cattcgttct
     7981 ggtttacctt cagccacctg gcgtttgctg aactatggcg tacagccaag caaatcaacc
     8041 acagtgcagg tcaccgattc cgttggaatg ctggaaacct atgctgaagt cgataagtca
     8101 ctggctgatc tgaacggtaa taccgccgaa ttccgcctgt ctgaagaccg cgcatttatt
     8161 gaagcgatga atcagcagat ggcgcagacg ttgttttatg gtgattccag cgttaaccct
     8221 cagcagttta tgggactgtc ctcccgctat tccagcctgt ctgcgggtaa tgctcagaac
     8281 atcattgatg ctggtggcac gggtacagat aacacctcaa tctggttagt ggtgtggggc
     8341 gaaaacaccg tgcatggcat cttcccgaaa gggcagaagg ctggcatcca gatggaagat
     8401 aaaggccagg tgacactgga agatgttaat ggcggcaagt acgaaggcta ccgtacccat
     8461 tacaaatggg acaacggact tgctctgcgt gactggcgtt atgttgttcg cattgcaaac
     8521 atcgatgtca gcaatctttc agaaccttcc tctgccgcaa atattgcgaa gttgatggtt
     8581 aaagcactgc atcgcattcc aaaccgtggc atgggtcgcc cggtgttcta catgaaccgc
     8641 actgtaggcc aggctcttga tctgcaatct ctggagaaaa catctctggc tatcagcgta
     8701 aaagagactg aaggcgagtg gtggacttca ttccgtggtg taccaatccg tgaaactgat
     8761 gcgcttctgg aaacagaagc ccgcgtggtg taacgcctgt tattaacctg tgggtcgtaa
     8821 cagacccact aatggagaaa gaagatgatc accgacaaac tgttgatgtt ctccgaagct
     8881 caggcggtta cgaataccgc ggcttctact gacgtaatcg atctcggtcc aattgacgga
     8941 aaacgtcgtg atatcggcgt aggttacccg cttgagtttt gggcgctggt taacacagcc
     9001 gccgcggcaa gcggtgatgc aactgtaaac atccagttgc agacgagtga gaataacagc
     9061 tcatggacca ctatttatga tagtggcgca ctggcaaaga ccgccctgac agcaggtaaa
     9121 cgagttgttt ctgcaaaggt gcctgccggt gttcagcgat atctgcgtgt taactactcc
     9181 gtcgcaactg gcccactaac ggctggcgaa ttcactgcgg gtatcagtct tgatgttgat
     9241 gccaatacgc cgtatccgat ccgctcaaaa gtaactggtt aaggtgatat tgatgtcagg
     9301 tgagaaacca agataccgcg ttctgcgcct ctctcatatc cataacactc tgtggccgga
     9361 gggggcagaa atcgaatacg aaggtgagcc tggtagcgca ctggaacctg ttaacgatgc
     9421 agccagacag gcaaaagcaa aagttgcagg aaaggtgtca atggcagcaa ccagcaccaa
     9481 aatcatcaac gatgtgtcag atgatggtga actggataag ctccgtgaag agtacgaatt
     9541 gctctttaac gagaagccac accataacgc caaagccgaa acgctccgcg agaagatcgc
     9601 agataagcgt aaagaactgg gcgtgtaagc ctcgcgaatc agacaagggg cttcggcccc
     9661 tttattgcag gagtgtatat ggaactcgta aacctcaaaa ccggcactga cagctaccag
     9721 gatgagagcg gagaaaccag aactcgcgat gaatacccgt gggggctgtg catcacgctg
     9781 aataacgaca cattgaataa gctgaaggcg caacctcagg gcgtcggaac agaagtgatg
     9841 ataactgcaa aggctgttat tcgaggcctg tctgccagag aaactgacga tggtgttaat
     9901 cgcagcgccg atctgcagat cactgatatg gcaatcgctc ctgtttccgg tgatgtagaa
     9961 aaatcagcgg ctgaaactct gtacggcaat gggggtgagt aatggcctct gtagtagaga
    10021 tctgcaatcg tgcgctgtcc aatattggca acagccgcag cattaacagc ctgacggaag
    10081 ccagcaagga agcgggggaa tgttcgctgc actttgaggc ctgccgtgat gctgtgcttt
    10141 ctgattttga ctggaacttt gctaccaaac gcgtggcgct tgcagatacg agcaatccac
    10201 cgcctgactg ggaatatgcg taccagtacc cgtccgattg tctgcgcatt actgaaatta
    10261 tgcttcctgg tgtacgcaat ccaacagcag caatgcgcgt tcagtacgaa gttggtgcag
    10321 acaccaacgg aacaggaaaa ttgatctaca cagaccagcc gcaggcatgg ctcaagtatg
    10381 tctctcgcgt ttcagatgtg aacatgtttg atgccatttt tatggaggcg ttggcctggc
    10441 gtcttgcggc agctattaac atggcgctga ctgggaatgc agacctcggt acgtttgccc
    10501 tcaatatgta caatcgcgag attcttagtg ctggctcgca tagccagaat gaatcacagg
    10561 aacctcagcc accggttgat gagtttacca ttgcgaggtt gtcctgatgg ctatcagttg
    10621 gatccagccc agctttgctg gtggtgagat tggaccgtcg ttgtacggac gtatcgacat
    10681 ggcgaagtac caggtggcat tgcgcaagtg cgataacttt atcgtgcggc agtatggcgg
    10741 cgttgagaat cgacctggta cgcgttttgt cggtgccgcc aaatacccaa atcggaaatg
    10801 ccgcctgatc ccgttccagt tctcgacggt tcagacttat gctctggagt tcggacacca
    10861 gtacatgcgc gttatcaaag atggtgcgtt ggtgctgaac agcagcaatg ttatttatga
    10921 aattgccacg ccatatactg aagccgatct gttcagaatt aaattcacgc aaagcgcaga
    10981 cgtgcttacg ctggttcatc cggcataccc gccgaaagag ttgcgccgct atgcgcatga
    11041 caactggcaa ctggttgatg tggtaacgaa gaacgggcca tttgaagata tcaatattga
    11101 cgagtcagtg acggtttatg ccagcgccag caccgggaca attacgttaa cggcaagcgc
    11161 ctctattttt ggcgtggagc aggtaggcaa attgttctat ctggaacagc cagcagtgga
    11221 ttcagtaccg gtatgggaaa ccagtaagag tacgtcgatt ggcgatattc gccgtgcaga
    11281 cagtaactac tatcgcgccg ttacagcagg caaaacatgc actttgcgcc cttcgcatac
    11341 agaaggcaca tcatgggatg gctggggcgg atccggtgat gatgataccg gcattgagtg
    11401 ggaatatctg cacagtggtt ttggcattgc ccgtatcact gctgcaaatg gaactactgc
    11461 aactgccgag gtgatttcct atatcccttc gcaggtagtt ggtgcgaata atgcaagcta
    11521 taaatgggct aaatatgcct ggaacagtgt taacggttat cctggcactg ttgtttatta
    11581 tcaacaacgt ctttactttg ccgcatcgac tgcgttccct cagactatct gggccagccg
    11641 taccggtgat tataaggatt ttggtaaaag caatcctacg caggatgacg acagaattat
    11701 ctacacctat gccgggcgtc aggttaatga gatccgtcac ctgattgatg tcggttcgct
    11761 ggtggcactg acttccggag gtgagtacgt catcaccggc gaccagaaca aagtgttaac
    11821 cccatcatca tttgcattca gctctcaggg atcaaatggc tcgagcaatg tcccaccaat
    11881 tgccgtggcg aatattgctc tgttcgtcca ggagaaaggc agtgttgtcc gtgatctggc
    11941 ctactcattc gatgttgacg gctatcaggg gaacgacctt actatccttg ccaatcatct
    12001 ttttcagaag cacagcattg ttgactggtg cttctcgatt gtcccttact ccagtgcctt
    12061 ctgcattcgt gatgacggta aattactggt gatgacctat ttgcgtgatc agcaggtttt
    12121 tgcatgggca ccacaatcca gtaccggaaa atatgaaagc acatgcagta tcagcgaagg
    12181 caatgaagat gcggtgtatt tcgtcgttaa ccgaaccgtt aacgggcaaa cagtgagata
    12241 catcgagcga ctgtccagcc gtttatttac cagcgatgaa gatgctttct ttgttgattc
    12301 tggccttagc tatgatggaa gaaatacgtc tgacagaaca atgaccatca ctggtggttc
    12361 tggtgaatgg gattaccgta cggaatatac aatcagtgtt tctggtggtg cgtacttcac
    12421 cagtagtgat gtcgacgcgc aactacagtt cccttatacc ggaactgatc ctgatactgg
    12481 cgatgaagtg tcaaaagaat tacgttgcga cattatttct gtaaccagca ataccgctgt
    12541 agtggttcgt gccaacagga acgtcccgcc atccctcagg aatgcggcca ccacgaactg
    12601 gcagatggcg cgccggacat ttggaggcct gtctcatctt gaaggccaga ccgtaaacat
    12661 tctctctgat gcgaacgtgg aaccacagaa agtggtttcc ggaggtgccg tcacgctgga
    12721 atcaccgggg gctgtagtgc acatcggcct gccaataact tctgaattcg aaacactgga
    12781 tatcaacatt aacggacagg aaacactgct ggacaaaaaa caggtgatcc cgtccgttac
    12841 cctggtggtg aatgccagtc gcggcatctg ggcgactacg cccggcggta aatggtacga
    12901 atatccacag cgtgaattcg agttctacga tgatcctgtt gatgacgcta ccggaaaagt
    12961 agatgtgaaa ctggacagta actggggaaa aaacggacgt gtaagaatcc gtcagcttga
    13021 cccgttgccg ctgtctgttc ttgccgttat tcctcgcctt actgttgggg ggttctgatg
    13081 atcgatgttc gaattattcc cgctaccgaa gagcatcttc agatgatttt gccggatgtt
    13141 cgtcaggctg atattgacga actgtatgcg gtatcgctga tgactaccga agatgcgcta
    13201 cgtgttggtc tgcgcactgc gactatggcc tggtcagggt ttgcgaacag agaactggta
    13261 accatgtttg gtgtatcacc ggcgtcaatg attggtggca atggtacgcc ctggctggtc
    13321 ggaaccagcc gtattgaaaa atatcagaag acatttctgc gccactgccg ccctgtattg
    13381 cagcagatgc tggcagttta tccgcgcctg gaaaactacg tcgacgagcg aaaccatgtt
    13441 gccaaagcat ggctgcactg gcttggattc aggcttgaag aagccgcgcc ttatggtgct
    13501 cttggtctta atttccacag atttcacatg gagagaaaat aatgtgtgat ccggttattg
    13561 ctggtggcgc aatgcttgcc atgagtggca ttcaggcata cacccagtac caacagggaa
    13621 agtatgcctc gaaggttgca gaagcgaacg cagatatagc cactgctcag gcaaatgatg
    13681 caataaacag aggtaacgct gaagctgagc aacggcgcag agagacccgg cagcggcttg
    13741 gcacacaagc ggcgacaatg ggggctaccg gcgctgattt atctaccggt aacgcgctgg
    13801 atatatttgg tgacactgct cagtttggcg ctcttgatgc gctgacgacg gtgaataacg
    13861 cgcaacgtga ggcttacggt tatcaggttc aggctgccaa ctataaagca gaagccagtt
    13921 cagcccgtaa acaggggaat gtgggagcag caacaacatt gctcactgcg cctctgaagg
    13981 catacggtgc gtaccagatg tttggtggga cgtggagtcc gtttactcaa agcactcatg
    14041 cgccaatcgg ggcagcagca ggaaccagat tacccggagg attataatgc cagtcgtacc
    14101 aacagtatcc ggacgccagg ttgagagccg tggagttcag tcagcaggct tgcagacgtt
    14161 ttctcagcca ggtattggtg atgcttttgt tcgggcaggg acagaggcaa ttgatgtttt
    14221 ggggcaggca aaacagcgtg ccaatatcgc tctggctcag gaggcatctc ttaacctcag
    14281 tcagataagc agtgatctgc tgaataaccc tgaaacaggt ttgcttaacc tgaaagggaa
    14341 aaatgctact ggaaaaggtc aggagtatac gcagcagttt gatgcccagg tcgagcaact
    14401 ggctatgtcg ctgccggatg aacaagctcg taatgctttc atgcagcagg cgcagcagca
    14461 gcgcattcag ttcactacgc aggccgggcg gcacgagata gggcaaataa atgcctacga
    14521 agaaggccag tttcaggcga cgctgctgaa caatggtaaa aatgccgcag cattgtatgg
    14581 cgacaacgcc gcatacgtat tggctaataa gcaaactttc cagcaaattg aggattacgg
    14641 cattgcgcat ggctggagcg acgagcaaat ccaggccaag aaaatcgagt ttaaagagaa
    14701 ggttgctgat gccgcattgt cccagtggtc ggcaagcaat gcgaccgcat tcatccaaag
    14761 taatggcgag ttaagtgata ctgctgctgg agctcgccgt gctgtagcag ataatgactc
    14821 ttccgagcgt gcccgtggca tacgcaacaa taacccagga aatctcgaat acagcaaaac
    14881 taatccgtgg gtaggccaga ccggtgatga tggtcgattt gctaaattcg aaacacctga
    14941 gcacgggatt cgtgcattag ggcgtaacct gatgtcgtat cagcggcagg gtatcgatac
    15001 cgtcagcgag ataattaatc gctgggcacc gcctactgat aaaaatgaca ctatgtcgta
    15061 tatcaaagca gtgtgcgaac aacttggcgt ttctgctgat gagcctctcg atgcatcaaa
    15121 tcctgatacc ctgaaggcgc tttgtgcagc cattatccat catgagaacg gtagtcagcc
    15181 atacagtgat cagcagttaa ctgctggtgt cagtgcagca cttggtttat caacaattcc
    15241 aaccaacacc aaacgctata ccggtaatgc agcattcgat gcggcatctc ctgaggctca
    15301 ggcaagtttt atgcggcagg cggatcaact gcgtcggcag cagcaggctg aatataaaac
    15361 gatgattgac agccaggttc gtgatgcgac agctgcgtat atgcgtggcg ttgaatttcc
    15421 taacccacct ggtgaggatg attttattgc agcttatggt gtcagagaag gaaacctgcg
    15481 atataccgag tttaagaata cgcagatcgc cggacagtat ataggttctt tccgcaacat
    15541 gccgacaagc agcattacag catatgttga gcaattacgc ccggatactg gtgagacagg
    15601 ggaggggtat gcggcacgcg cagctcttta tgacaacgtt gtttcggctg caaatcaggt
    15661 gataaagcag cggcaatcgg atcctgtgca gttctctctt gccgccggac aggcaaagcc
    15721 tatcgacatg agcaataagg ataactttgg acagagcgtt gccttgcgtg ccgctcaggt
    15781 cagtgacctt gccaagtcat atggcactcc actgacgttc ttttccaaag acgaggccaa
    15841 tcagatcggt gttttctttc gtgatgcgcc cgtttcccaa caggcagcat atctcgatac
    15901 catcaggcaa agcactggcg gtgggcaggt gtatatgtca gcactacagc agatcagtgc
    15961 caacgctcca tctgctgccg ttgccgggat actgatggat aagcctggtg gtattttggc
    16021 agaaaaaaac tggtttaatc cggatgtttc cgtgtctcct gaaaccgctg cgcagacaat
    16081 tcttgctggc gcggcggctc gtaaaggtac tgatgatgcg aaaggtattc cgatgcctaa
    16141 agatgctgat cttcgccttg agttttctga catggtgaag gatgcatttg ctggtgatgc
    16201 tcagggggca tcaatggcat acgagatcgc aaaggactat tacgctggtg tgatggcgaa
    16261 aaaaggcgtg gtatcaggcg aaattgacaa tgatatctgg aaacaggctg ttaacgtagc
    16321 tacaggtggc gtgcatgact ataacggaat ggggaatgtt cttttgccgt ggggaatgtc
    16381 tgcagagcaa ttcgataagc aggttaatca ggcttggaat gaacaagttg tcggcacagg
    16441 gataaaaaca ccgcctggtc agtatggttt gcaaagttac ggcgatagtc agtacctggt
    16501 aaaacttggg actggatatc tgctgaagga tgatggttct cctgttgttc ttgatctgac
    16561 acagaagcgt cagagattct ccggagatat tccgcaatga gttactttgg ccttaatcca
    16621 gtaaaccaga atcagcagct tgacgaagca gcatcaaatc cagctggctt taacagcgat
    16681 gttggttttt tcgacaatgc tgtaggagcg gcattgtctg gtttgtactc cgggctggtg
    16741 gcaaagccag atcagttgct atgggcaggg atggataaaa tcgtatcccc gattgctcag
    16801 tttgttaacg aaaacacctc gctcaatgac acttcagttt catacattgc cgagcagaga
    16861 aaactagcag agcagcaggt taagcggctg acgcctgatg ccgcgacaac cggaaccgca
    16921 gggcaggttc tttatgggtt gttcgatatg ggcgggcagg ctgttgtcgg tacaacgctc
    16981 ggtggtccgg tcggaggtgc tgcggcggta acttcgctac agggtttttc tgagtttgaa
    17041 cgactgacag ctcagggggt tgatttcagg acggcgcagg aagcgggatt agtgcagggt
    17101 attactgctg gtgccggaac actgatccct atgagtctcg ggttacgtgc tggtggtgcg
    17161 ctagcggaag gtgtggcggc tcagcttgcg cggacgggtg aaagttcagt gcgacgcgcc
    17221 gcggcaacag cagtacgtgc aacgccagat attgcctatg ccgcaggtac aaatattgcg
    17281 ttcggtatgg cacagcgcgg gcttactgca aaaacgcttc gtgatggtgg ctatagcgaa
    17341 atggctaacc agtatgatgt gttggatcga caggcaattg ctattgatgc tgttcttggg
    17401 gtggcttttg gtggagtcgg cagatttatt aactctcgcg gcgagtctac aaacgcacca
    17461 aacttttcac cagttgatat cgatgctgca ctggcggcga atgccgctca tcatgctgaa
    17521 attgatattg ctcccggcgt gccgatcaac gtgctttcgc gcaattctca cattcaggct
    17581 ctgcgaaaag ctatgtctgc tgttagccag gggagacctg tagacgttgc cagcattgtt
    17641 gagtctgcat ctttcagtga aattcctggg cgcaagaatc tgctgtctca ggcagttaat
    17701 gaggctctgt catctgtaga tgatggagta acggcgcgcg ctatcgaaaa tcggttgctt
    17761 gaagaacagg ctgcgcagct tttgccgcgt ggcgatagac aggtttacca gtctgaaatc
    17821 gctaatagcc aacgaattct cgacaatctc actgaacagc gcgcacaaat tcttgcagaa
    17881 gagccaactg gtagcggtaa agctttgtct cgtgctcgat cagataaaca ggccagaatt
    17941 cgcgatattg accaacgaat ccggcaggca caagaacgcc tggaattttc ccgtaacgcg
    18001 ttggcgccgc acgagcatgg aggtcagttt tttgaagctc gagcagaact ggcacggcga
    18061 ctgcaggtag aaagtgaact taatgctcag gctgtttcat tctataaaac agcagaggtc
    18121 aggacgccag acgaagtagc tccttttgag cctgataaaa tattgcaaca ggcagaacaa
    18181 aaaatgatgg cggatcaggc aggagatatt gatctgcgca tagctgaaga ctcgttgctt
    18241 gaatcacctg acatgataat caccgtgctg gatgatgatg gtaatccaca atcgcgcagc
    18301 gcgcgtgaag tactggatga agcgaacagg gaaagtgagc aggcaataca ggattccagc
    18361 ctgtttgatg tcgctgtggc gtgtttcttg agaggttaaa ttaaatgaga caggaatgta
    18421 tacaagcggt ccagcaggcg gcgcagcgca cgttaacggc gcgagaaata cagaacattg
    18481 aagaccgcat ttatcgaaat atgcgctcca ttgctcgtga tgacccgatg tcgtggagac
    18541 aactttccga atcagagcgg ctatatcgtg cagcacaatt ggcatctgaa gaattacagc
    18601 gagaagcggc attaaagaaa cgtcgtgtgg ccctcactat agccgcacgt cagagattgg
    18661 ataaatttat caatagctat caaggggctg atgggaaact cggcgctctt aaccgtacta
    18721 ttgcttttaa tgcagacggt aaatcgaatt ttctctctgt tgagtccaga acaaaagcca
    18781 ctcgtgatta tgcattgagt caattgcaag aggcatttga agcagttgat cctcgctttt
    18841 ttggcctgtt tgaagatgaa gctggcgtgc gtgacctggt atatgaaatg cgagggcaaa
    18901 atactggcaa tgctaaagca agaaaaggtg ctaaggcgtg gagagaagtg acagatctac
    18961 tgcgccgccg gtttaatgat gctggtgggg acattggcta tctcgaaaac tggggaatcc
    19021 ctcaacatca ttctatggaa aaggttgggg cggtatcaaa ggataagtgg gttagcgatg
    19081 ttataggtaa gctggatcgc aaatattata tccgagccga tggacaactg atgaacgatg
    19141 ccgagttgtc tgcatttctt ggagaggctt ataacacgat cgctactggt gggctgaata
    19201 agcttactga taccggaatg cgaatttccg gcgcacgtgc taaccgtggt aatgcatcac
    19261 gacagataca tttcaaagat gcagattcct atctgcaata tcagcaactt tatggcgatc
    19321 gctctctatg ggaaatcatg gtcggtcacc tggaaggtat cagtaaagat attgcactgg
    19381 tggaaacata tggcccaaac cctgatcatg ttttccgctc tcttcttgat caggtgaagg
    19441 cagaaacggc aacagctaac ccgagtaaaa ccggtagcgt cgagcggctg gcgaacaaaa
    19501 cagagaacct gtacaacttt atttccggaa agacacagcc tgttgcgaat cctcacatcg
    19561 cgcgctggtc tgacaatatc agaaactggc tggttgccag caggctcgga tccgcgttgc
    19621 tgtcatcgtt ctctgatctt ggaaccatat atctgtctgc gaaggttacc aaccttccaa
    19681 tgaaccagtt attccgcaac cagcttgaag ctatggaccc aacgaaccgt actgagcttg
    19741 cgcgggcgcg ccgcgctggt ctggcgatgg aatctctact tggcagcgtt aaccgctggg
    19801 cgatggataa tatggggccg tctgtgtctc gttgggcggc aacggcggta atgcgtgcca
    19861 gtggacttac agcatggtca gatgcgcaca agcgcgctta tggcgtaacc atgatgggaa
    19921 gcctgggaga agtagtgtca cggacaccag accttcgtag cctcgatgac tctgattttc
    19981 gtatcctgaa aagcaaaggg attactgaca cagactggag cgtatggaag ctggcgcaac
    20041 aggaggactg ggggaacggt aataatacga tgctgacacc ggaaagcatt atgcgtatcc
    20101 ctgattcagc agttaaacat cttggtgagc ctgaacgtgt gaaatttgag gcaatgcgta
    20161 aactgctcgg tgccgtaact gaagaagttg atatggctgt tattacaccg ggcgcacgtg
    20221 aacagatgtt cgtagggtct ggtcttcagc gtggaacatg gaaaggtgaa ttaacgagaa
    20281 gtgttttcct gtttaaatcg ttccctatct cggtagttat gcgtcactgg catagagcta
    20341 tggggatgcc gtctgctggt gggcgtgcgg catatatagc aacgttttta gcaagcacaa
    20401 ccatgcttgg tgctctttcc atgcagatta ctgatcttat taatgggaga aatccaaaag
    20461 aaatgaccgg tgacaacatg gttaaattct ggataaatgc atttttaaaa ggtggtgggg
    20521 cagggttgta tggtgatttt cttttctctg accacaccag gtacgggagc ggcgcactag
    20581 cgtcgatgct tggcccggta gctggtctgg ttgatgacgt agtgaagatt gctcagggta
    20641 taccgttaaa tgctgtggaa gggaagaatg agcagactgg tggtgatttg gttaagcttg
    20701 ggaaaggttt gatgcctggt gcgaatctct ggtatttgaa ggcggctctc gatcacatga
    20761 tctttaacca gatgcaggag tatttttcac caggctattt gcgtaaaatg gagcaacgtt
    20821 caaagaaaga gtttaaccag acatactggt ggcgacctca ggatgtcact ccgcaataag
    20881 gaacaacaat gaaggaatta ttgctgttat cggtattttt gatatccgct tgctcgacat
    20941 cctatgatgt ttatgacggt gttgataagg catattgcga caaagttaaa atggattttt
    21001 ctcttgccaa gacggcgaag gatagttgta ttgatcacta cgtcaagact tataccaagc
    21061 cagcctcatc ggcatctgat attgctgaag gtgctgtgtt tgagtgtaac aaggtgatat
    21121 ccatcgcagc gagttcttca tacgatgctg ctgtatgtgc aatggctgaa agaaacggca
    21181 tgtcagtgca aaaaattaat agcatgataa gcagtaatga cgaagccaaa ataagaactg
    21241 atatcagctc ggtgaaaaaa gatgccatga acagagttgt aaaatatcag tcatctttat
    21301 aagtcgtgac atgtcacagg ccgctttcgc ggccttgttt ttaacgaatg ccaccgccgc
    21361 ccgggcggga atccgcagaa cgcccaccgc agcgggagcc gtcagcggca gtatcgctgt
    21421 cgtgctgaca acgaccggca aaggcctgag ttgaagctac cagagacaac aaaacgaaca
    21481 gtgcagcaaa tgcttttttc attgtgaaat ttccatctat aagccacctc gatgtggcgt
    21541 caatgagtgt agcactgact tttgtttcgt ccacaaaaaa gcccgcggcg cgggctattc
    21601 cttccattta tcagaaaaaa gatcttcttc taaaggcatt ggttctgttt ttgttttctc
    21661 aaagaattga taactgatag tgattgctgc ctctttaaac tcttcttgct cagttatgtt
    21721 gtgagcatct gcgtcaacaa aaaacataac cagcgcatca cgattgtgat ttaccgaata
    21781 aactaaaaag caatcacttg ttggtatgca ttttacttgt acagacgcta tatttttcca
    21841 tgcatcccaa gatgattttt taccagtgtg ttttttatca ctatcttctg gaatatagtc
    21901 ttggttatcg acatgagtat gcctgacatt tagtttaagc atttctgtcg gacgagcaaa
    21961 tgcagcatct ttaccaagag atggatggta tcccgttttc caatactggg taaaggcatc
    22021 agatactttc ttcagctcaa gatcagatgc acaaagggcc gagaaatttt gcgtatgcaa
    22081 tactcgaccc ttatatctga caatttgatt tttatcattc tgcgacgcag acgaactcat
    22141 aattttcctt attgttacga gaatcaaaga aagcgcggga tacgcgactt gcgtgatctt
    22201 ttgtcatagt aacttttacg tactctacgc ttccattgaa agaacgtctt gcggcagctt
    22261 gcgctctacg catctgcaat ttttcgtttc gcatgacatt acctcatatc tcataagttc
    22321 attacacgga ttaataaaaa tgaaaccaat ccgtttaccc ttgaggtaat agtacgctat
    22381 tcacccacag tctgcaatct gtacagaatt atttaaaagc acatccatgt gccaccgccc
    22441 gtcagaagaa tcctgccttg tcgttgatgt attccgcgtg ggtctggata tcacgcaggc
    22501 atttactcac accgacgatg tagcagaaca tggtggtcag ttccgccgcc gcgcccgata
    22561 cgtcatgccc gtcttcctgt aactggttca gcagattcat cagcagtgag ttctccgtca
    22621 ggccgagaac accagacggc gagtgaatca ggctgcggta gccgggcttc agtggggcac
    22681 tgtaggtttt gttctctatc ttcattgcct gtattactgc tgacgccgtg gcgttggcta
    22741 cctggtcggc aaccatcttt atgcgttctt cctgcgggag cgagttttta atgtaacttc
    22801 cggtgcggcg gatctgagga agaacctcac ctgtaaccca tttacgaaag cggtagggga
    22861 tagtgcctgg tgtcaccgca tcgcggcagc ggaggatcag tgtgtagagg cctgactcgg
    22921 agatgatgtt gatctctttt actcggctgt caaaaattgc acgatgttca tgccctatgt
    22981 tgaacataga cctttcatca tcatccagtt tttcaagtga ttgggtgacg ttttggatac
    23041 gcagcgcact acaaacgtct tgggctacaa accatggttg gccatcgata atgatggaac
    23101 ggatagggtt aacagattca aatttgaaga tggcagtttg agcattagcc atggtggtta
    23161 tctccactta gtgattttaa tcaccaccgc aacgccaatt actggtggtg aactggacaa
    23221 ggttggcgta ccggcctaag tggtaccggc gtcctttcgg acccctgccc agcccaccat
    23281 aattcggata tggctgtgct taacgcataa aaaaaccacg tctggcgtgg tatgcgccac
    23341 ttagtaactc gggacgccaa tcccggcact ggattttgcc agtacccgat tactatggca
    23401 caagaggagt gcgatgtaaa tttacctcaa aggtaaatat aagcactcca cttggtaatt
    23461 gcaaacctta tctggtttgt tttcgtaatt gttcggcaca atagtcgaga tgtgtttgca
    23521 gatcctgcat agacatctgt gagctggtga cgtagttaat cagtgcagtc agttcggcaa
    23581 gtgggccatc gacattaaat ccatccttat cgagatcccg gagtaatttc atcaagagcg
    23641 atccctccac cagtaatctg acgcctcccg gcgtgtgaat cctttcggta aatccgtctt
    23701 ccagtggata gtgatactgc tgcatcttat cttctccatg caataactgt acaaatatac
    23761 agtatcaaat aatttgtttg ctatccagca cgttttgcaa atcacctgaa aggtaatatc
    23821 tgtgtgtatt gatgatgttt ctatccatat atggttcgtt gggtaataaa atagccagat
    23881 atgcggcgca acgggtgctg cgactatctg gagatttagc atgacggtct caaccgaagt
    23941 tgaccacaac gaatacacag gtaacggtgt tacgacatca tttccgtata ccttcaggat
    24001 tttccagaaa tctgatctgg tagtacaagt ggttgacctt gatgaaaata tcactgttct
    24061 tgctcttgat actgattaca cagttaccgg ggcgggagga tataacggtg gtaatgtaat
    24121 tctgtcgaag gtgttggcta acggttatct gatttctata tatcgagagc taccggttac
    24181 gcaggaaact gatttgcgta atcagggtaa gttctttgca gaagtgcatg agaacgcttt
    24241 tgataagcta acgatgctga tccagcaggt tcgaaattgg ttcagtctgg cgctacgaaa
    24301 gccgtcgttc gtggctaatt attacgacgc gatgaataac tatattcgta atcttcgtga
    24361 cccatctagg ccgcaagatg cagcaacgaa aaattatgtt gacgggcttg cagaaacaaa
    24421 cctgagcaga acgctacgaa ctcccgaacc gatatcaact ttgcccggcg ttgagcaacg
    24481 taagaataag attgttgcaa tggatgattc aggagaccca atcatggttc tcccggaatc
    24541 tgggagcgct actgatgtca tgttgcagct tgctgccacc gatgggttta aaaatatcgg
    24601 ttcctgccct gatgtggcca cattaagaaa tactgaacct tcgtcagtaa accagcaaat
    24661 tattgtcaga ggttattata gcgatacccc cggtcttgga ggtggggtgt tcatgtcgtt
    24721 tgacagttct gaagctgacg atggtgtgaa tatttttgtt acccccggcg gtaagcgatg
    24781 gaagagagct ggaagccaca tcgatattcc ggtagagaac ggtggcatga tgacatcatg
    24841 tacggctgag caaaacagtg aagcctttga acgcctgact gcatgccttc catatgaagg
    24901 aggtacgctc agactcaatg gattttacga tattaagtat ggcgctatcg tgcctccacg
    24961 tgtcacgctg gaggggtgcg gtatggattc ctgtggatta attaaaacag gtaacgatat
    25021 taaaaccgtt ccggatcgca tgtggcaggg tgtaccacac agcttctcca aagactttat
    25081 tgttgcggta gatatggata gtgacacatc aggtgattta agcgggaaac aaacaagaag
    25141 caccagaata attggattga gtattctaaa tactgcgccc aatccttgtg attacgggat
    25201 atatagcgct atcagttaca atgtgcgcct tcaggacctg tatattcata gggtgaaaac
    25261 cggttatcgc actagtgata gctggttgca gacatgggaa aacattacta ttcaggatgt
    25321 cgttactgga gttaaaatcg aagcaggtgg aaccacatat aatttaaaca atatttatgt
    25381 gaaaacatgt tcaggtgttg catatgattt taataacatt acatatagca gcctgacttg
    25441 tttggctgct gacttcgtta atggtactgg ctataaattt aaagattgta ctggagtaaa
    25501 tctatctgga tgcggaagcg aagaaggcac tggtaaaata tttgatataa atggtagtcg
    25561 aattaacgta actgcattta gaggggttaa tcatcatgat ggaggagttg aagctgtatc
    25621 tctggaatcg tgtttcatct cattggtttc atctcatttt tctggttact ctggagtctc
    25681 atctagcaaa tacatatatc ttaatgatgc aactgcaaat tttaatggtg cggtaattcc
    25741 cgacaacacc actgattcta ttagatgggg gagtgggcag agctggatca acaaggttga
    25801 ttcaggcggc acctatagta taaccggaaa ctcatcatgg actgcattat gcagacttga
    25861 gtcaggatat ataacggttc tatcagtatc accacccacg ggagatacaa ctaatttccc
    25921 gtttggcacc aagtgggaat tgcttggtcc ggtttctacc ggaccgtgga agtggacata
    25981 cgtatcaact ggtgaatgga aggtatccgc aactaccacg taaaaaaagg ggctgaaagc
    26041 ccctttttat ctaatatctg aatttgtttg atatcttttt cccaatatcc attgctggtt
    26101 tttctatcgc aataaatgat atcagtccag caataagtga tagtgctatg cataaaactt
    26161 gatttaagta cagatttata tcaggtaata acctgaatgt tatctgctgt attaaaaagc
    26221 cccacaagta aacgccatat gatacatcat gttttattct tattgatttt attatttttg
    26281 ttgttgataa atatacgaaa aatgtacatg cgcaaaaata aaaaagcata tgctt
//
LOCUS       DAZFFE010000066        25950 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFFE010000066 DAZFFE010000000
VERSION     DAZFFE010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.066"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..589
                     /locus_tag="ACHJ69_002903"
     CDS             <1..589
                     /locus_tag="ACHJ69_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HFO5015850.1"
                     /translation="PETRYISTIGLAQIAANQFLHVYRPRHWINACQAGPLGWTMPAA
                     LGAVKADPSVPVVAISGDYDFQFLIEELAVGAQFNLPYIHILLNNAYLGLIRQSQRAF
                     DIDYCVQLSFENINAPEINGYGVDHKAVVEGLGCKAIRVFASQDIAPALQEAQRLRDE
                     FHVPVVVEIITERVTNIAMGPDINKVTEFEEILDL"
     gene            602..1384
                     /gene="hyi"
                     /locus_tag="ACHJ69_002904"
     CDS             602..1384
                     /gene="hyi"
                     /locus_tag="ACHJ69_002904"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO5015851.1"
                     /translation="MAKFAANLSMLFTEYPFIERFAQASHAGFHGVEYLFPYDFSADE
                     LVSQLHQHNLTQVLFNLPAGNWQEGERGIACHPGRAKEFQEGVSRAIEYALALNCSQV
                     NCLAGKHPGGYSHEQCHETLVENLRYAVDKLASHGIKLVLEAINTKDIPGFFVNNTRQ
                     ALNIIHDVNHPDFRYQYDIYHMQIMEGNIATTIKNNLNNIGHIQLADNPGRHEPGTGE
                     INYPWLLNYIDQIGYQGWIGCEYIPSTTTTESLRWLKNETQF"
     gene            1402..2289
                     /locus_tag="ACHJ69_002905"
     CDS             1402..2289
                     /locus_tag="ACHJ69_002905"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO5015852.1"
                     /translation="MNIGFIGTGIMGKPMAYNLQQAGHTLYFSAHFEPAPQELIGERG
                     IVCSTPREVAQECEVIITMLPDTPHVEDVLFHPNYGVIHGLSHGKIVIDMSSISPVAT
                     KAFAQRIIAGGAEYIDAPVSGGEVGAKAGTLSIMVGGCEEVYLQIKPILELMGKNITL
                     VGNVGDGQTCKVANQIIVALNIEAVAEALLFASKSGADPARVREALMGGFASSRVLEV
                     HGERMIKGTFEPGFRISLHQKDLNLALENARLLNTPLPNTATTQQLFSACAALGGKEW
                     DHSALIRALETQANFTIRK"
     gene            2364..3506
                     /locus_tag="ACHJ69_002906"
     CDS             2364..3506
                     /locus_tag="ACHJ69_002906"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFO5015853.1"
                     /translation="MKIVISPDSFKECLPAWKVAEALATGWRKVLPGSQLVCLPVADG
                     GEGTLETLIHATDGTFYTKKVTGPLGESIHAQYGILGNQTTAVIELAQASGLELVSPV
                     QRSPLYTTSFGTGELILAALEHNIDTVILCLGGSATNDGGIGLMSALGASFTDAEGLS
                     VSVNGMGLAAIHHIDLQHLDPRLKNVKFIAACDVTNPLTGDNGATRVFGQQKGASADD
                     LEQLEQGMKNYARCIYRCCGKEVDTIPGSGAAGGVGAALMAFLDARLQPGISLVLEAI
                     QYTQHLKYAALAIVGEGKLDSQSLNGKAPVGAAKVAQMMGVPVIAIAGYIDDQLDLNE
                     LRQCGIEACFSVVNGPCDLPTALSQGESNLIHLGENLAGYFRAILS"
     gene            complement(3562..5103)
                     /locus_tag="ACHJ69_002907"
     CDS             complement(3562..5103)
                     /locus_tag="ACHJ69_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFO5015854.1"
                     /translation="MQYLKKRFSLIELNVFIPAILFIAVIILCLTIYPQDTSRYINKI
                     HHFLTWEMGGIFLVMTFLVVLCCLWLAFSRYGDILLGQSGEKPDFSLLTWLGLIFTSG
                     TGGSLLYLASVEWIWIIQQPPFGATAGSAQAARWASAYGMFHWGPSAWAWYLICAVPI
                     GWFMHVKKTNSLKVSDLCRGCLGARADGFCGHCVNFFYMFGLLGGAVTSLALGTPMIS
                     AVFCHVFHLDPAGQFINVMVIFIWTLVPLFILFFGLKKGVAWTSNWNIRADILMLLAI
                     LICGPTAFILNQSIDGLGLMLQNFVAMSLSTDAIGRSGFPQMWTVFYFSWWVVYAIPF
                     GLFIARISKGRTIRQLIVCGTLAGSLGCMVFYMVLANFGLSLQTTHVIDFVPILNEQG
                     RGVVVSRLLEQLPASQVFLVAFGAIALISYITGHCTVGYALGFATQKRADSESEPAFW
                     NVAFWLIMTGIVAITLYLLDAQSLQPLQTVSILAGLPLRGVVFILLKSFLTQLAAEEK
                     NARDE"
     gene            complement(5127..5351)
                     /locus_tag="ACHJ69_002908"
     CDS             complement(5127..5351)
                     /locus_tag="ACHJ69_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015855.1"
                     /translation="MRDDDLEHLSANYWITTVLCGLFLGIFLFMLQQHQRGLALYCIA
                     ALSLICAFFSLRLSRQLQHMKKERRNIRPH"
     gene            complement(5404..6768)
                     /locus_tag="ACHJ69_002909"
                     /pseudo
     CDS             complement(5404..6768)
                     /locus_tag="ACHJ69_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228116.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(6786..8054)
                     /locus_tag="ACHJ69_002910"
     CDS             complement(6786..8054)
                     /locus_tag="ACHJ69_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5015856.1"
                     /translation="MTSNWKRWITLALCGIAGSAIYKLPYLRETYYDAMQQATGATNA
                     ELGFLMTAYGLVNFLLYLPGGWAADRFSARKLMTFSLISTGISGFYYATFPSYTMICL
                     LHALWAVTTVFTFWAVCVRIIRTLGTSEEQGRLYGYWFLGKGLTSIVLGFLSVPVFAK
                     FGEGVDGLRATIIFYSVVTILAGVLAWFVCQDETHSEDKANFRLADMAFVLKMPTVWL
                     AGVVTFCMWSIYIGFGMVTPYLTQILHMGESEVAVASILRAYVLFAMGGLIGGQLADR
                     CASRTRFMIYAFIGMIVFTTVYFFLPGESRYVTIALANMVALGVFIYSANAVFFSIID
                     EVRIPAKVTGTAAGLISLLTYFPEIYCYTMVGNMVDRKPGIAGYQDVFLFMLVCAFIG
                     LIAALVLQRVNRKSKNQVNEITENNADCAS"
     gene            complement(8121..9203)
                     /locus_tag="ACHJ69_002911"
     CDS             complement(8121..9203)
                     /locus_tag="ACHJ69_002911"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HFO5015857.1"
                     /translation="MDKIIISPAKYIQGNGSLDNIATYAASLGTEPLIIADEFVTGLV
                     GDRVSQSFARENIIADFDVFCGECSQNEISRIRKKFNQRKYNVVIGIGGGKTLDTAKA
                     VAYYQKIPVVVVPTIASTDAPTSSLAVIYTPEGQFSEYLFFPKNPDMVIMDTGVISAA
                     PVRLLVAGMGDALSTWFEARANQASGKATMAGGASTLAALAIARLCYTTLLEDGYKAK
                     VAVEQGVSTKAVENIIEANTYLSGIGFESSGLAAAHAIHNGMTQLEECHHCYHGEKVA
                     FGVLVQLVLENAPQEEIETVLNFCHSVGLPTNLHMLGVKEINKDKLRAVAEAATAEGE
                     TIHNMPFVVTAQDVLCAILTAHHLGL"
     gene            9528..10592
                     /gene="dhaK"
                     /locus_tag="ACHJ69_002912"
     CDS             9528..10592
                     /gene="dhaK"
                     /locus_tag="ACHJ69_002912"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO5015858.1"
                     /translation="MKKLINQIDSVVTEQMEGLIATWPHLQANYAPRYVWCKQTDNAV
                     ALISGGGSGHEPLHAGFVGMGMLTGACPGEIFTSPTPDQMIECAKAVDNGSGVLFFIK
                     NYTGDILNFETAVEMLHEEGIAVGTVIIDDDVAVKDSLYTAGRRGVAGTVFVEKIVGA
                     AALQGYNLRQCEQLGKDVNNATRSFDIALSACTVPAAGKPSFELADNEIEFGVGIHGE
                     PGIERRTLQDLNTLIDSVIAQLLDNTPWRRTLRHWDRHAGGWIDASSMNESFDQNAEY
                     IVLINGLGSTPESELYGVARVFMCAAQRQGIKISRQLVGNYCTSLDMAGFSISLLKCT
                     PEFLQLWDAPVNTPALRWGC"
     gene            10605..11237
                     /gene="dhaL"
                     /locus_tag="ACHJ69_002913"
     CDS             10605..11237
                     /gene="dhaL"
                     /locus_tag="ACHJ69_002913"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO5015859.1"
                     /translation="MKIQNKHVIAWLESCAAHLTEQQDFLTALDRDIGDADHGLNMNR
                     GFSAVKATLPDIERQHIGNILKNTGMKLLSSVGGASGPLYGTLFIRASAAVGARTELS
                     LEEWLACLEEGIAGVIARGKAEQGDKTLCDVWVPVLHEAKKNLQAGMSPSLLLNTMVQ
                     DAATAVDNTINMQAKKGRASYLGARSVGHQDPGATSSWLMIKAMQEGFAG"
     gene            11241..13621
                     /gene="ptsP"
                     /locus_tag="ACHJ69_002914"
                     /pseudo
     CDS             11241..13621
                     /gene="ptsP"
                     /locus_tag="ACHJ69_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(13669..14052)
                     /gene="iraD"
                     /locus_tag="ACHJ69_002915"
     CDS             complement(13669..14052)
                     /gene="iraD"
                     /locus_tag="ACHJ69_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO5015860.1"
                     /translation="MMRQSVQTVLPESTGNNTLSLRDSVCRDLFQLFSSPHSPLPILL
                     VSGMPEWQGHNQSDKLLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHELAISLEMM
                     LYHDDEPLTFDLVWQKGSWHRTMPQ"
     gene            complement(14466..15239)
                     /gene="uxuR"
                     /locus_tag="ACHJ69_002916"
     CDS             complement(14466..15239)
                     /gene="uxuR"
                     /locus_tag="ACHJ69_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO5015861.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNADSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(15454..16914)
                     /gene="uxuB"
                     /locus_tag="ACHJ69_002917"
     CDS             complement(15454..16914)
                     /gene="uxuB"
                     /locus_tag="ACHJ69_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO5015862.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGNETIADTMTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(16995..18179)
                     /gene="uxuA"
                     /locus_tag="ACHJ69_002918"
     CDS             complement(16995..18179)
                     /gene="uxuA"
                     /locus_tag="ACHJ69_002918"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO5015863.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            18628..18735
                     /locus_tag="ACHJ69_002919"
                     /pseudo
     CDS             18628..18735
                     /locus_tag="ACHJ69_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18978..19880)
                     /gene="fimH"
                     /locus_tag="ACHJ69_002920"
     CDS             complement(18978..19880)
                     /gene="fimH"
                     /locus_tag="ACHJ69_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO5015864.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYNGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGVVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(19900..20367)
                     /gene="fimG"
                     /locus_tag="ACHJ69_002921"
     CDS             complement(19900..20367)
                     /gene="fimG"
                     /locus_tag="ACHJ69_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO5015865.1"
                     /translation="MLALASATIQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSF
                     SLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAVDSTGYYKNQGTAQNIQLELQDD
                     SGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(20416..20946)
                     /gene="fimF"
                     /locus_tag="ACHJ69_002922"
     CDS             complement(20416..20946)
                     /gene="fimF"
                     /locus_tag="ACHJ69_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO5015866.1"
                     /translation="MRNKPFYLLCAFLWLAVSRALAADSTITIRGYVRDSGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(20956..23592)
                     /gene="fimD"
                     /locus_tag="ACHJ69_002923"
     CDS             complement(20956..23592)
                     /gene="fimD"
                     /locus_tag="ACHJ69_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO5015867.1"
                     /translation="MSYLNLRLYQRNTQCLHIRRHRLAGFFVRLFVACAFTAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAQLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(23658..24383)
                     /gene="fimC"
                     /locus_tag="ACHJ69_002924"
     CDS             complement(23658..24383)
                     /gene="fimC"
                     /locus_tag="ACHJ69_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO5015868.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMVVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(24420..24959)
                     /gene="fimI"
                     /locus_tag="ACHJ69_002925"
     CDS             complement(24420..24959)
                     /gene="fimI"
                     /locus_tag="ACHJ69_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5015869.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPVNWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(25023..25571)
                     /gene="fimA"
                     /locus_tag="ACHJ69_002926"
     CDS             complement(25023..25571)
                     /gene="fimA"
                     /locus_tag="ACHJ69_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO5015870.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTTVADKAAIAFLGTAID
                     GTHPNVLALQSSAAGSATKVGVQILDRTGAALALDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         6761 a         6566 c         6049 g         6574 t
ORIGIN      
        1 accggagaca cgctatattt cgacaattgg tctggcgcaa atcgccgcaa accaattttt
       61 acatgtttac cgcccacgcc actggattaa tgcctgtcag gctgggccgc taggctggac
      121 aatgcccgca gcactgggcg cggtaaaagc tgatccatct gttcccgttg tggcaatatc
      181 cggtgattac gattttcaat tcttgattga agaattagcc gtcggcgcac aatttaatct
      241 tccgtatatc catattttac ttaacaacgc ttatcttggt ttgattcgtc aatcacaacg
      301 tgcatttgat attgattatt gcgttcaact gtcatttgaa aatattaatg ctccagaaat
      361 taatggttat ggcgttgatc ataaagctgt ggttgaagga ttaggatgta aagcgattcg
      421 cgtattcgca tcacaggata ttgcgcctgc actgcaagaa gcccagcgtt tgcgtgacga
      481 atttcacgta cctgttgttg tagaaattat tactgaacgc gtgactaata tcgctatggg
      541 acctgacatt aataaagtca cagaatttga agaaattctc gatttataag gaaacaggat
      601 tatggcaaag tttgcagcaa atttatctat gctctttacc gagtatccat ttattgaacg
      661 ttttgcacag gcaagtcacg caggattcca cggcgttgag tatcttttcc cctatgactt
      721 ttccgcagat gagttggttt cgcaactgca tcaacataat ctgacgcagg ttctgtttaa
      781 tcttcctgcg ggtaactggc aagagggtga acggggtatt gcatgtcatc ctggtcgggc
      841 aaaagaattt caggagggcg tttccagggc aatagagtat gcactggccc ttaattgttc
      901 acaagttaat tgccttgcag gaaaacatcc aggtggttat tcgcatgaac aatgtcatga
      961 aacactggtc gaaaatttgc gctatgcagt agataaattg gcttctcatg gcataaagtt
     1021 agttctggaa gccattaata caaaagatat tcccggtttt tttgttaaca acaccagaca
     1081 agctctcaat attattcatg atgtaaatca tcctgatttt cgctatcaat acgatattta
     1141 tcatatgcaa atcatggaag ggaatattgc tacaacaatc aaaaacaacc tgaataacat
     1201 tggacatatt caattagccg ataaccctgg gcgtcatgaa ccagggacgg gagaaattaa
     1261 ttatccgtgg ttgttaaatt atatagacca gattggttat cagggatgga ttggttgcga
     1321 atatattcca tctacgacaa cgactgaaag tctacgctgg ctaaaaaacg agactcaatt
     1381 ttaatccctg aggtatttat catgaatatt ggatttatcg gcaccggcat aatgggtaag
     1441 ccgatggctt ataatttaca acaagccgga catactcttt atttttcagc gcattttgaa
     1501 cctgcaccgc aagagcttat tggtgaacgt ggcattgttt gctctacacc gcgtgaagtc
     1561 gcgcaggagt gtgaggtcat catcaccatg ctccctgata ccccacatgt tgaggatgtt
     1621 ctttttcatc ccaactatgg cgtaatacac ggtttaagcc acgggaaaat tgtgattgat
     1681 atgagttcaa tttccccggt tgccaccaaa gcatttgcac aacgtattat cgcgggaggc
     1741 gcggaatata tcgatgcgcc cgtctctggc ggtgaagttg gcgcgaaagc cgggacgctt
     1801 tccattatgg ttggcggatg tgaagaggtt tatttgcaga tcaaaccgat tctggaattg
     1861 atgggtaaaa atatcactct ggtcggtaac gtcggtgatg gtcaaacgtg caaagtcgct
     1921 aaccaaatta ttgttgcact gaatattgaa gcggtggctg aagccctgct ctttgccagt
     1981 aaatcaggtg ccgatcccgc ccgcgtacgt gaagcgctga tgggagggtt tgcctcctct
     2041 cgcgtgctgg aagttcatgg cgaacggatg attaaaggaa cattcgaacc cggttttcgt
     2101 attagtctgc atcaaaaaga tctgaatctg gcgctggaga atgcccgcct gttgaatacc
     2161 ccgcttccta acacggcgac gacacagcag ttatttagcg cctgcgccgc gctcggtggt
     2221 aaagagtggg atcattctgc gttaattcgg gcgcttgaaa cacaagccaa tttcactatc
     2281 cgtaaataat gaattaaggg aaccaccggt tcccttcgtt cactcattgc cctgccccat
     2341 tcccctattt tgagagacct gctatgaaga tagttatctc ccccgactcc tttaaagaat
     2401 gccttcctgc atggaaagtc gccgaagcgc tggcaaccgg ctggcgcaag gtcctgcctg
     2461 gcagccaact ggtgtgtttg cccgtggctg acggcggcga aggcacgctc gaaacactca
     2521 ttcatgcgac tgacggtacg ttttacacta aaaaagtcac cggaccgctt ggcgaatcaa
     2581 tacatgcgca atacggtatt ttaggcaacc aaaccaccgc agtgatagaa ctggcacaag
     2641 cttcagggct ggaactggtt tctcctgtcc agcgttctcc cctttatacg acgtcgtttg
     2701 gcactggcga acttattctc gccgccctgg aacacaatat tgataccgtt attctatgcc
     2761 tgggtggcag tgctacaaat gatggcggta ttgggttgat gtcggcactt ggcgcatcgt
     2821 ttacagacgc cgaagggcta tcagtctctg ttaatgggat ggggctggcg gcaattcacc
     2881 acattgactt acagcacctc gatccacgat tgaaaaatgt gaaatttatt gcagcctgcg
     2941 atgtcaccaa cccactaacc ggcgataacg gcgcgacccg ggttttcggt caacaaaaag
     3001 gggccagtgc tgacgacctt gagcaactgg aacagggaat gaaaaactat gcccgttgca
     3061 tctaccgttg ttgtggtaaa gaagtcgata cgatacccgg ttctggggcg gctggcggcg
     3121 ttggcgcggc cttgatggct tttctcgatg ctcgcttaca accgggtatt tcgctcgtgc
     3181 tggaagcgat tcaatatacc caacatttaa aatacgcagc attggccatt gtcggtgaag
     3241 ggaaattaga tagtcagagc ctgaatggca aagcgcccgt aggcgcagcc aaagttgccc
     3301 agatgatggg cgttccggtt atcgcaattg ccgggtatat cgatgatcaa cttgatctga
     3361 atgagttacg ccagtgtgga atagaagcct gtttttccgt cgtcaatggt ccttgtgatt
     3421 tacccaccgc gctgagtcag ggagaaagta atttaattca tcttggggaa aatctggcgg
     3481 gatattttcg tgcgatcctg agttaattcc cgttcggtaa aatgcccgat aatgcaaatt
     3541 accgggcatt cctgaacatc attattcatc tctcgcgttt ttctcttcag ccgcaagctg
     3601 cgtcaaaaaa ctcttcaata aaataaacac cacgccgcga agcggtagtc cagccagaat
     3661 agacaccgtt tgtagcggtt gcagactttg cgcatcaaga agatagagtg tgatggcgac
     3721 gattccagtc ataatcaacc aaaatgccac gttccagaat gccggttcac tctcactatc
     3781 tgcccgtttt tgcgtcgcaa aaccgagggc ataacccaca gtacagtgtc cggtaatata
     3841 tgaaattaat gctatagccc caaaagcaac caaaaacacc tgacttgcgg gtagctgctc
     3901 cagtaaacga gaaacgacaa cgcctcgccc ttgttcgtta agtatgggaa caaaatcgat
     3961 gacatgagtt gtctgcaacg ataagccgaa attagccagt accatgtaaa aaaccatgca
     4021 tcccaatgaa cctgccagag ttccacatac aatcaattgc cggatcgtcc ttccttttga
     4081 aatacgggcg ataaataaac cgaatgggat ggcatacacg acccaccatg aaaaataaaa
     4141 tacggtccac atctgtggaa atccgctacg accaatagca tcggtactta aactcatcgc
     4201 tacaaaattt tgcagcatca ggccgagacc atcaattgat tgattaagta taaatgccgt
     4261 tggtccacaa atcagtattg ccagtagcat caaaatgtca gcacgaatat tccagttact
     4321 ggtccatgcc acaccttttt taagtccaaa aaagagaatg aataatggta ctagcgtcca
     4381 gataaaaata accatgacat tgataaactg cccggcagga tccagatgga acacatggca
     4441 aaatacggca gaaatcatcg gcgttcccag cgccagagac gttaccgcgc cgccgagcaa
     4501 accaaacatg tagaaaaaat tcacacaatg cccgcaaaaa ccatccgcac gcgctcccaa
     4561 acatccacga cataaatcac tgacctttaa tgagtttgtt ttcttaacat gcataaacca
     4621 accaatagga acggcgcaaa tcaaatacca tgcccatgcg gacggccccc aatggaacat
     4681 gccataagca gaggcccaac gagcagcctg agcgcttcct gctgttgcgc caaaaggcgg
     4741 ttgctgaatg atccaaatcc actctacaga ggccagatat aacaagctgc cccccgttcc
     4801 agaagtaaaa ataagtccaa gccaggttaa caagctgaag tcaggttttt ctcccgactg
     4861 acctaacaag atatcgccat aacgtgagaa ggccagccac agacaacaaa gtacaaccag
     4921 aaaagtcatg accagaaaaa tcccgcccat ttcccaggtt aaaaaatgat gtattttatt
     4981 tatataccgg ctggtatctt gtggatatat cgtcaaacac agaatgatta ccgcaataaa
     5041 taatattgct gggataaaca cattcagttc aatcaaagaa aagcgttttt ttaaatattg
     5101 catttcagtc tcctgaatgt tttcattcaa tgaggacgaa tatttcgccg ttcctttttc
     5161 atgtgttgca attgtcggga aaggcgtaaa gaaaaaaagg cgcaaataag tgaaagagcc
     5221 gcaatacaat acagagctaa accacgctga tgctgttgca gcatgaacag gaatatcccc
     5281 aaaaagaggc cgcagagaac tgtggttatc cagtaattgg cacttaagtg ctccaggtca
     5341 tcatccctca tggtctttct cctgcaaggc gcagacaggc tttcagctct ctgcgccttg
     5401 ttgttatgcc agacgtacgc cctgctgttg cagcgtgttt tgcaaatcag ggatagcaac
     5461 ctgacgcacg gtgcaatttt gctgtagcga aaccacggcg gcagtacctg cggcctcacc
     5521 ggtaacggca caacacatca tgttacgcat agaagtatgt gcaactacgc ctgcggaaat
     5581 acagcgaccg gcgaccaaaa ggttctccac tttttgcggc accagacaac cataagggat
     5641 ctgaaagaaa cgcccggtcg ttggcaaaat gagataaccg gaaccatcaa taaattccgg
     5701 gaaaatccct acactgtccg aacaacgccc ctgattaagg acgtaatcat cagcaatacg
     5761 tatttccccc tcaataagcc gtgattcacg cgtccccagc gtggcaccaa aattgcgtaa
     5821 ccgtacattt tcaaagcccg gaacatagtg gcgtagtgcg tcaattgccc ataatgcttg
     5881 ctgccttccg gcaatctcag ctttggttaa atcgaagaca tcggtacagt caaaaccaaa
     5941 ggcgtacacc atattcatct agaaagcctc accgctttca gaaaaggtcg accaagttcc
     6001 ggcaatcgcc tgggcgtcac ctggaatcac accatcctgt tgcgcgcggg taaaaatatc
     6061 ctccatatac gggctaaaca tcgggtcttc tttacccgtg gtttgaatcg tccagttttt
     6121 gccccaatcc gcgtaggtcg gcttaagttc ttccgcaaca aaacggttaa agcgtgccac
     6181 atcaacacct gcgcaactga acatcacggt gacgcccatc agttggtcct tgctccgttt
     6241 cgtccagggc gcgcctgcac gagcggcaat atcagcatcc cccgtgcaat cgacgattct
     6301 tttcgccaga attgcccctc gacctgattt attctcgacg ataacaccgc ataacgtatt
     6361 cccgtccttg atcacgtcca caacccagga gtgatataac ggctcaacgc cagattccgt
     6421 aatcatctga tcggcgacaa ctttaaatcc ttcagtatct ataacttcgc taattgactg
     6481 aggttctggt cgtgtgaaac ccagcgctcg tgcgcggcct tcatactcac gacagatccc
     6541 ttcacaatct tccgtccccg aatgacgata ccaagcaaaa ctttctaccc cgacctgcgt
     6601 caatacgccg cctagcgacc cgtagcgctc aacaatcatg gttctggcac ctaaccgccc
     6661 tgccgcaatg gcagcggata atcctgccgg accgcccccg ataaccagaa cgtccgtttg
     6721 tgcaatgact ggcgtttgcc gtgcactttc attaatcatg tccaccattg gttcccccgt
     6781 cagattcagg atgcgcaatc cgcattgttt tcagtgattt cattgacttg atttttactt
     6841 ttacgattca cacgctggag gaccagtgcg gcgataaggc cgatgaatgc gcaaaccaac
     6901 atgaacagga aaacatcctg ataaccggca atacccggtt tgcggtcgac catattgccc
     6961 accatcgtgt agcagtagat ttccgggaaa taggtcagta aagagatcag acccgcagca
     7021 gtccctgtga cttttgccgg gatccgaacc tcatcaataa ttgagaagaa cacggcattc
     7081 gcagagtaaa taaacacgcc caacgcaacc atattcgcca gcgcaatagt gacgtagcgc
     7141 gactcaccgg gcaggaagaa ataaacggta gtaaagacaa tcatgccgat aaaggcgtaa
     7201 atcataaaac gcgtgcgtga cgcacagcgg tcggccagtt gtccgccaat taatcccccc
     7261 atcgcaaata acacataggc gcgaagaata ctggcaactg ccacttccga ttcgcccata
     7321 tgcaggatct gcgtgagata cggcgtgacc atgccaaagc cgatataaat tgaccacata
     7381 caaaaggtca caacacctgc cagccaaacc gtcggcattt tcagcacgaa tgccatatct
     7441 gccagacgga aattcgcttt atcttcgctg tgtgtttcat cctgacaaac aaaccaggct
     7501 aaaacaccgg ccagaatagt cactacggaa taaaaaataa ttgtcgcccg taaaccatca
     7561 acaccttcgc caaatttggc aaataccggc acggaaagaa atcccagtac aatggaggtt
     7621 aatcccttac ccagaaacca gtaaccgtat aagcgtcctt gttcttcgct ggttcccagg
     7681 gtacgaataa ttcgcacaca cacggcccaa aacgtaaata cggttgtaac agcccaaagt
     7741 gcatgtaaca aacagatcat ggtatacgag gggaaggtcg cataataaaa accgcttatc
     7801 ccggtggaaa ttaatgaaaa ggtcattaat ttacgtgcag aaaatctatc ggcagcccag
     7861 ccgccaggta gatataataa aaagttcacc agaccatagg ctgtcattag aaaaccaagt
     7921 tcagcattag tcgctcctgt agcctgctgc atcgcgtcgt aataggtttc ccgtaaataa
     7981 ggcaatttat atattgcact tccggcaata ccacataacg ccagcgtgat ccaacgtttc
     8041 cagttactgg tcatatttat tcccgaatga agtatttaaa atgacaccaa cggcctgctt
     8101 tgcacaggcc gtattatttt ttaaagtccc aaatgatgtg cagttaaaat tgcgcaaaga
     8161 acgtcctgcg cagtgaccac gaatggcata ttatggatag tttccccttc agcagtcgcg
     8221 gcttcagcca ctgcccgtaa tttatcttta ttaatttcct tcacgcctaa catatgaaga
     8281 tttgtcggca gaccaacgct atggcagaaa ttgagtacgg tttcaatttc ctcctgtggt
     8341 gcgttttcca gtacgagctg taccaggacg ccaaatgcca ctttttcacc gtggtagcaa
     8401 tgatggcatt cctccagttg cgtcatgccg ttatgaatgg catgagctgc cgccaggccg
     8461 ctactttcaa atccaatgcc gctaagatag gtattagctt cgataatatt ctctaccgct
     8521 ttggtgctaa caccttgttc aaccgcgact tttgccttat agccatcttc cagtagcgtg
     8581 gtataacaga gtcgggcaat agccagtgcc gccagtgtcg acgccccacc tgccattgtg
     8641 gcttttcctg acgcctgatt ggctctcgcc tcaaaccagg ttgacaatgc atctcccata
     8701 ccggccacca gcagacgcac tggcgcggct gaaatgacgc cagtatccat aatcaccata
     8761 tccgggtttt tcgggaaaaa cagatattcg ctaaactggc cttccggggt gtaaattact
     8821 gccagagaac tggttggcgc atctgtggaa gcaattgtcg gcaccaccac taccggaatt
     8881 ttttggtaat acgctacggc ttttgccgta tccaacgttt taccgccgcc aataccaata
     8941 acgacattat atttacgctg attaaatttc ttacgaattc gagaaatttc attttgcgag
     9001 cattcaccgc aaaaaacatc aaaatccgca ataatgtttt ctcttgcaaa gctctggctt
     9061 actctgtcac ccaccagacc ggtcacaaat tcatctgcaa taattaatgg ttctgttccc
     9121 aaactcgccg catatgtagc aatattatcc agagaaccat ttccctggat atatttagcc
     9181 ggacttataa taatcttatc catatcattc ctcgcgtaac ttgttttgct tcactgttaa
     9241 cctactcgtt gcatccagtg gcggcaaaca tttcattcgc gaaaaattat gttttatgat
     9301 cgcgctgtaa aaaacatcat cccaaagcaa taataaaaat attttttatg ctttaatcac
     9361 aaacccctaa tcttgtcacc gatcccattt cattttggaa caacacaatg aattaactgt
     9421 ttcattatga atcttattat cgtgcgattt gttttattgc ggctggagaa ttaatctcgc
     9481 aagcatattt agcgcgattt acgtaattta tcagaacagg aatgactatg aagaaattaa
     9541 ttaaccagat tgattctgtt gtcacagaac aaatggaagg actcatcgcc acctggccac
     9601 acctgcaggc aaattacgca ccccgatatg tgtggtgcaa acaaaccgat aatgcagtgg
     9661 cgctgatttc aggcggcggc tcaggccatg aaccactgca tgcaggtttt gtcggtatgg
     9721 gaatgttaac cggtgcatgt cctggtgaaa tcttcacatc gcccacacca gaccaaatga
     9781 tcgaatgtgc caaagcggtt gataatggtt ctggcgttct gtttttcatc aaaaattaca
     9841 ctggcgatat tctaaatttc gaaacggcag tcgagatgtt acacgaagaa ggcatcgccg
     9901 ttgggaccgt cattattgat gatgatgtag cggtaaaaga tagcctgtat acagccggac
     9961 gtcgcggtgt tgcgggtaca gtttttgttg aaaagattgt cggtgcggct gcacttcagg
    10021 gatataacct gaggcagtgc gagcaacttg gcaaagatgt taacaatgcc acccgttctt
    10081 ttgacatcgc actgtcagcc tgtacggttc ccgccgcggg aaaaccctca tttgaactgg
    10141 ctgataatga aatcgaattt ggtgtcggga tccacggtga acccggcatt gaacgccgca
    10201 ctttgcaaga tctgaacact ttaatcgaca gcgtgatagc gcaattactg gataataccc
    10261 cctggcgacg cacattacgc cattgggatc gccatgcggg gggatggatt gacgcatcat
    10321 caatgaatga gtcattcgat caaaatgccg aatatattgt gttaattaac ggacttggca
    10381 gcacgcctga atcagagtta tatggtgtag ccagggtatt tatgtgcgcc gcacagcgcc
    10441 agggaattaa aattagccgc cagttagtcg gtaattattg tacatcatta gatatggccg
    10501 gattttccat tagcctgctg aaatgtaccc cagagttttt gcaactttgg gatgccccgg
    10561 tcaatacgcc agcgcttcgt tggggatgtt aataaggaca ttatatgaaa attcaaaaca
    10621 aacatgttat tgcctggctg gagagttgtg ctgcacatct tactgaacaa caagattttc
    10681 taaccgcact tgacagagat attggtgatg ccgaccacgg cctcaatatg aatcgtggtt
    10741 tcagtgccgt gaaagcgacg ttgcccgaca ttgaacgtca gcatattggc aatatcctta
    10801 aaaatactgg catgaaacta ctttccagcg ttggcggtgc cagtggtcct ctttacggca
    10861 cattatttat tcgcgcttct gcggcagtgg gagccagaac agaattaagt ctggaagagt
    10921 ggctggcgtg tctggaagaa gggattgcag gcgttatcgc acgaggaaaa gcagaacaag
    10981 gggataaaac actgtgtgat gtctgggttc cagtcctcca cgaagccaaa aagaacctgc
    11041 aagccggaat gtcgccttcc cttctgctga atacgatggt gcaagatgcg gcaacagccg
    11101 tcgataacac catcaatatg caggcgaaaa aaggacgtgc cagttatctc ggcgctcgca
    11161 gtgtgggcca tcaggatcca ggcgcgacct cctcctggct gatgattaag gctatgcagg
    11221 aaggattcgc gggataaatt atggttgcca ttgttattgt ttctcatagt ctacgtctcg
    11281 cccaggggat tgaggaatta gcactacaga tgtctggagg ggacgttcct ctggccattg
    11341 ctgcaggtat tgatgatccg caaaatccga ttggcactga tgctattgcc attatgtctg
    11401 cgattgaatc ggtctggtca ccggatggcg ttctggtact catggatatg ggcagtgcgc
    11461 tgcttagtac cgagatggcg ctggagctgc tttctgaaga gcaaagaagt gcaatttatc
    11521 tgctcgccgc ccctgtagtg gaaggtgcca tgtcagcggt aacgtctgcc gcagcggggc
    11581 tttcggtgac ggagattatc gctgaagtgg atcttgcgct gtgcgctaaa cagcagcagc
    11641 tcatgccatc cccgcctgca ggcgaggtta ttccagcaat atcaccagtc aatcattccg
    11701 gtgaatggca aactttttgc tggacgatat gtaatcccca cggcattcat gctcgtccgg
    11761 cagccagcat cctgaaagta tcagcacaat attcggcaaa cattatcgtc atcaaaggcg
    11821 ataaacgcgc cagcacccgc agtctcaacg aactggcaat gctgggcgtc cgctgtggcg
    11881 atgaaatcat cttccaggcc gaaggcaccg atgccagtga cgctctcaag gcaattgaaa
    11941 cgttggcgaa aaatcatttt ggtgaagctc atctgctcgc ttcttcagag attccgctaa
    12001 cggaaacgcc ggtggtcagc gcccctgtcg atggcgcaat atcaggcttg tcagtacaaa
    12061 atggtatcgc tattggtcct gttaaatggt ttacctgcga acgaccagaa attacgcagc
    12121 gcaccgtcga ttctccgcaa gaagagctgt cgcgaatcga gtctgctatc gacattgttg
    12181 tttgtgaact tgccgataaa gcggcaggcc ctgaaggcga tattttgccg cccataaaat
    12241 gatgcttgaa gatccagaga ttaaccggca attacagcaa cgtctggcaa agggcaaaca
    12301 ggcggaattc gcatggttag aagtcatgca ggcattagca gagcaatatt gtcaggcgga
    12361 aacactgtat ttacgtgaac gcgaggcaga tatccgtgac cttacacgtc aggtactgaa
    12421 ccagctttgc ggggtaagtg aacaacactt tatcactacc gcgccctgca ttttgttagc
    12481 caacgatctg ttgccatcac agataaccag cctgaataaa gcacacattc ttggcatttg
    12541 tcttcataat ggcggaacaa cttcacatac agcgatcctt gccagagcaa tgggtattcc
    12601 tgccattgtg aaggcagcta ttaccccgca aaacgtccgc gataacgaca cggtcattct
    12661 tgatggtgaa acaggccgtt tgtggctaca gccggatgag gtcacccgcc tggatttact
    12721 gcaacgcgcc gaagcatggc ggcaacaaag ggatcgtcag ttagctgatg ccatgctgcc
    12781 cgctgtcacg caaggtgggc gtaaaatcag cgtgctggcg aatattggtg atctgcaaga
    12841 cattgatgcc gcactgtctc acggcgctga gggcgttggc ttgctgcgta ccgagttttt
    12901 attccacgat tctgcgacat tacctgatga ggaagagcag ttccgtgtgt attgttctgt
    12961 cgcccgagtt tttggcgaca aaccagtcac gatccggact ctggacattg gtggtgataa
    13021 accgctacct tcataccctt tacccacaga ggacaacccc ttcctgggac ttcgaggtat
    13081 ccggttgtgt ctggcgcatc cacaaatatt tattcctcaa ttgcgcgcac ttatcagggc
    13141 cgggaaagaa tacccaacgt tgcaaattat gctgccgatg gtttcaacgc tcgaggaggt
    13201 cagggccgtc aaaacattga tacatgctca ggcaaaactg ctgggactta ctgcagaaaa
    13261 tcttccggcg ctgggaatta tgattgaggt tcctgctgca gtcatgattg cagaaaaatt
    13321 agccagtgaa gtcgatttct tttccatcgg caccaacgat ctgacgcaat atattatggc
    13381 ggcagacaga ggaaatagca ccgtcgcgga actggtcgat tatcgtaatg acgcggtgat
    13441 taacgctatt gctatggtgt gtcaggccgg acgtaataat gaaattccgg tcagtatgtg
    13501 cggcgaaatg gctggagata tccagcaaac agcacgactg ctcaccatgg ggattgataa
    13561 gctcagtgcc agtccatccc ggcttccggc cttgaaagca gcaatcagaa cctcccacta
    13621 attgtgacct aacgcgatac gtcttgcgcg tatcgcgttt cccgtcattt actgcggcat
    13681 cgtgcgatgc cagctcccct tctgccagac caggtcaaac gttagcggct catcatcgtg
    13741 gtacagcatc atctccagac taatggccaa ctcgtggcaa taggcttctt tcagattgac
    13801 ctgcaatgcc gcaatacgtg gctcatgaaa taaaagcgcg gaacgtaact ggcggcaata
    13861 ccaactctgg agaagcttgt cgctctgatt atgcccttgc cactcaggca tgcctgaaac
    13921 aagtaatatt ggtagcggtg aatggggaga actaaaaagt tgaaagagat cccggcatac
    13981 ggaatcacgc agcgataacg tattgtttcc tgtactttca ggtaataccg tctgaactga
    14041 ttggcgcatc attttgcgca ctcctgacgt ttagtggcat ctgtatcctg ccctggctgc
    14101 cggacaccac tgggttgatg ctcatattcc ataatcattc ctttatgctc attgcgtccc
    14161 tggcactttc ataagggatt tataagatcc gccaaagata aaacactgta cagaaaagaa
    14221 tatccactga cttttaattt aaaaaggtat catttgacaa aagtcattaa acataacgga
    14281 aatacgttaa catattctga ttattccgaa taatttctca gcgttaaact gatatataat
    14341 tattccaaaa caatatatcg tacgtaatga ttaatttacg cataatatat tctgcagaac
    14401 ataattaact attcagggaa catacatagt gcaaagcggt taaactttgc accagaaatg
    14461 agggatcagg cgtcgacttt atccagcggc catgaatcaa acagatagcc atcgaaatag
    14521 atgtcatcaa cattagagaa ctccagcaag cgctgcttca cattctccag atgctgccac
    14581 attgccagct tcgccgctcg ggcatctttt ttgattaacg cggcgaggat ctgtttgtga
    14641 tcgcccaacc actctttgcg atacaggctg tcatccagat ggctgtgcaa ctggatccac
    14701 attggattgt tttcccgcca ctgccaggac tgacggaata actccaccag catgctgtta
    14761 tgcgttgctt cggcaatagc gagatggaac tgcatgtcac cgctttcgct gctgcccggc
    14821 gcgctggaag ccagttcacg ctcttccagt tgcaatgcct ggcgcatttt gacgatatct
    14881 tcgcgggtag cctgcagggc ggcaaactcg gcgatgttac tttccagtaa ctgccgcgcc
    14941 tgtaacagct caaaaggacc ggcatcgttg cagacgttgg catccggact gtctgcgttg
    15001 tggctgcctg agctatcaag aacatagata cctgcacccc gacgcacttc caccagccct
    15061 ttgatctcca gcatgatcag cgcttcacgt accaccgtcc gcgtgacatc aagcatttct
    15121 gcaatttcac gctccggtgg cagccgttcg ccaggattgt acggcgtctt tatgatcaga
    15181 tcgcggatca tcgccccgac ttcctggtaa ggtctttgcg cagaggtggc agatttcatg
    15241 gtgttgtgtc cgttaggtgc ggttaatcaa attttttccg gatgcggcgt gaacgcctta
    15301 tccgacctac aacgggcaat tttacggcac ccgttaagct caaactatac cattagcact
    15361 ggatgttggc acgtacaggt gggcaaaaaa acgaaaactg gtcaaccaaa tattagtttg
    15421 attgaccagt aagtctgtag ggtaatcagt tagttacagc gcagccacac actcgcgcgc
    15481 accgcgctca cacagctgct gatatgccgc cgtcaccgcg ccgacaaagt cggcgttctg
    15541 cggcagatca tcggcaaaga taccgctcag gcccagcagt gctttcacgc ggtctgcacc
    15601 ctgatactgc gcgttgatct tctggaactc cgccagcatc gggtcaacca cgtcaatggc
    15661 attaccctgc tcatccacgc cctgggtgta gcgcatccag cccgccacgc ccagcgccag
    15721 gtggcgccag ctaccgccgt tttgcaggtg cagacgcacc gggtccagca gacgctgcgg
    15781 taacttctgg ctgccgtcca ttgcaatctg ccaggtacgg tgacgcagag acgggttgct
    15841 gaaacgctcg atcagcagcg tcgcataggc gttcaggtcc gtaccttccg gcatcgacag
    15901 cgttggcgct tgttcctgca tcatcagggc aaaggccgct ttgcgataag ccgggttagt
    15961 catggtgtcg gcaatagttt cattgccgcc gaggtaaccg aggtacgcca ggaaagagtg
    16021 gctaccgttc agcatacgca gcttcatcat ttcgaacggt acaacatccg caacaaactg
    16081 cgcgccgact ttatcccagt ccggacgacc attaacgaag ttatcttcaa ttacccactg
    16141 acggaacggt tcgcaggcaa tggcgcacgg gtcgtaaaca cccagctggt cagcaatttc
    16201 ctgtaaggtt tctggcgtcg ccgccggaac gatgcggtca accatggtgc acgggaaggt
    16261 gacgttttct tcaatccatg ccgccagttg cgggtcacgc gcctgagcca gcccaagaac
    16321 ggcaaccttc gccacatgac cgttttcacg cacgttgtcg caggacatca ccgtaaacgc
    16381 tttcagccct ttttcacgac gcagacgcag ggcttctacg atgtaaccaa tcgcagactt
    16441 cggcgcagtc gggttttcga gatcgtgctt gatcagcggg ttattgagat ccagctgacc
    16501 gcttgccgca tcagcgcagt agcctttttc cgtgaccgtc agagagacaa tcgccgtctg
    16561 cggacgcgcc atcgcgttga gaataccttc gcaaccgtcg atttccggat gcagcgcttc
    16621 tttcatcgaa ccgataattt tcagctcggt gctttccgcg cctttttccg ccacggtgta
    16681 cagcagttgc tgttttttca ggttttcgat cagcacgcgg tcgttgcctg gcatcaggtt
    16741 aacttcgcag ataccccagt cgctgtcggt actttccagc agatgatggg tatacagcgc
    16801 ctggtgcgcg cggtgaaacg ccccgcaacc gagatgcaca atgcgtgatt ccagacgaga
    16861 atgatcccat gacgggcggg caaccggcag attgctgtca acaatagtag tcatcgtaaa
    16921 ctccagaatc cgtcttcaga gagtgacggc aaggaagaag gaaccggcac ggcgcgcagc
    16981 catgccggtg ggtattaacg gctaaagaaa gcgcgctgga tcgccagttc gacaccgcga
    17041 acttcggcca ggcctttcag acgaccaatt gcggagtaac caggattggt tttcttcttc
    17101 aggtcgtcca gcatctgatg accgtggtcc ggacgcatcg ggatcaggtc ttctttgcct
    17161 tccgctttac gacggtgttc ttcttcaaca atcgctttca ccacttcgta catatcaacg
    17221 tcaccgttca ggtgcgccgc ttcgtggaag gttttcgggt tatcttcacg catggtggag
    17281 cgcagatggg tgaagtaaat acgcggaccg aactgcttga tcatatcaac cagatcgttg
    17341 tcagcacgca cgccgtagga accggtgcac atggtgaaac cgttcgccat gctgtttacg
    17401 gtatcaacca tccactgcat atcttcaatg gtggaaacaa tgcgcggcag gccgaggatc
    17461 gggcgcggcg gatcgtccgg gtgaaccgcc atacgcacgc caacttcttc agcaactgga
    17521 ataatcgctt tcaggaagac agcaaagttt tcgcgcagtt tggctttgtc gatatctttg
    17581 tacagctcca gatgcttacg gaactggtcg agggtatacc cttcttccgc acctggcaga
    17641 ccggcaatga tgttacgggt cagacgcgct ttgtcttcgt cgctcatagt ggcgaagcgt
    17701 tcagcggcct gagcaatttc ttcttcggtg taatccgctt ccgcgcctgg acgcttcagg
    17761 atatgcattt cgaatgcagc gaattcgatc tggtcgaagc gcagagcttt ggagccgtct
    17821 ggcagcacgt attcgaggtc agtacgggtc cagtcgagca ccggcatgaa gttgtagcac
    17881 acggtgcgaa tgccgcactg cgccaggttg cgcagggtct gctgatagtt agcaatccac
    17941 tgctcatagt tgccagtgtg ggttttgata tcttcgtgaa ttggcacgct ttctacgaca
    18001 gaccatacca ggcctgcgtc ttcaacgatc gccttgcgtt tgaggatctc ttcaacggac
    18061 catacttcgc cattcgggat atggtgcagc gcggtaacca cgccagttgc gcccgcctga
    18121 cggacatcag ctaaagaaac cggatcgttt gggccgtacc agcgccaggt ctgttccatg
    18181 atttttcctc tttcacatag tgggttcaac agcgaagaga cgcggaaccc cggagttgtt
    18241 taggtatccg gatgaacaac ccggccacag ccgtagcgtc gcatcggatg agatggcgta
    18301 taagttctac cttttggtaa gacatgtcaa cccgaattct aaaattggtt aaccacatca
    18361 caagaattta actctcctgg ttgttgctgc aaattgcagc aaagggtaat cgatggttat
    18421 tgatcagagg atgtgacatt caccgcaaca atggttgacc aatttacata acatgtcgcc
    18481 caaaataaca acggttcaac caggtaaggt aaaacgttat gcatgtgctt aacattctct
    18541 gggtggtatt cggcattggc ctgatgctgg tactgacaat gaccgcaggt tcagacacat
    18601 tccctacatt agcaatgcct cttagattac tttgacctgt cagtaaaaga cacgttgaaa
    18661 acctgaggac tgctggagct ggtcaactcc gtggttgggc tgattattgt gttgattatt
    18721 agcatggtag cgtaaaagag caccagagcc tgccattggc aggctctgac tgatgacaaa
    18781 gtctaaaaat gtgcccggcc agtcccctcg cccctccggg gagagggtta gggtgagggg
    18841 aacagaccag caaagtgcgc tttgtttatg ccagatgcga cgctgacgcg tcttatctgg
    18901 cctacaaagg gctaacgtgc aggtttttag cttcaggcaa tattgcgtac ctgcattagc
    18961 aatgccctgt gatttcttta ttgataaaca aaagttacgc caataatcga ttgcacattc
    19021 cctgcagtca cctgccctcc ggtacgtgcg taatttgccg ttaatcccag acttaccgcc
    19081 gaagtcccta ctactcctaa cgataccgtg ttattcgctg gaataatcgt accgttgcgc
    19141 gtcaactgta cgccgacgcc ctgcgctggt gaaaacgacg cggtattggt gaaaattgag
    19201 ttgcccgcat ctgcggttgt gccggagagg taatacccca ggttttggct tttcgcacaa
    19261 taaacggtaa gaggaatcgg cactgaacca gggtagtccg gcagagtaac ggtgacatca
    19321 cgagcagaaa catcgcagcc gccagtgggc accaccacat cattattggc gtaaatattc
    19381 cacacaaact ggaaatcatc gctgttatag ttgttggtct gtcgcaaaat aagcacggca
    19441 attaatgagc cagctttaat cgccactccc cccgcactgc tcacaggcgt caaataaagc
    19501 gccaccggcc acggcttatc cgttctcgaa ttataaacaa cccgcggcgt ttcgctggta
    19561 gtagggaaag gatagctact gccattatat tttacggtcc cggaaaaatt agataacacg
    19621 ccgccataag ccgagcctcg ttgcagtgtg acataatctg taatggtttc cgggtaatcg
    19681 ttatggcaaa agatttgcgt cgaaagatcc acgaccaggt tttgccccac attcacggca
    19741 ggcgcaaggt ttacataaac attggcgctg ccaccgccaa tagggattgc ggtaccattg
    19801 gcggttttac aggcgaatga ccaggcattt accgaccagc ccatcagcag tacagcaaac
    19861 agggtaataa ctcgtttcat tacaatcatc tctttgggtt cagctgtagg tataggtgat
    19921 gctaatcact gcctgaatgg ttccctgagt ggctccgcca tttaccgtca atgctctgac
    19981 ctgtaacggg aagtgcgctg attgtgagga atcatccacc tgaactgttt tggttgcgcc
    20041 agtattcaat gtgttgccac tgtcatcctg tagctctaac tggatgtttt gcgcggtccc
    20101 ctggttttta taatatccgg tactgtcgac tgccccgctg aagctggcag tgaccctcga
    20161 cgttcccacc ggacaattag tcaactcaag cgcaacatca tgccaggccg atgccgcccc
    20221 ggcagacata agactgaaag aataaagatc gccgagatct accgtggcat tggtggtgga
    20281 aaccgtgcac ggtttggcga cgaccttacc gttcaccgtg atggtgacat cggctgcctg
    20341 tatcgtcgca cttgcgagcg ccaacattgc cgccaataaa tacccacgtt agcaccattt
    20401 catgagcatc tccagttact gatattcaag agtgaaggta gccgtggcat tgatatgccc
    20461 cgcagtgaca ggcacctgtg tcgccattag ccgggcgtaa aaattcagcg tatttggttt
    20521 acccggcgtc agggtcgtcc acgaaatcgc ggacgatgga gcattaaggg gtatttgatt
    20581 ttgctgctca ttcagaagct gtattcccag tcccgcagcc gctgacaccg tattttcaag
    20641 tgcaagcagg ttggcattgt ggctatctgc aacgccagta aacccaacct ttacggcaga
    20701 aacggcgtta ccacagggtg acagcaaaat acgaaatgga acgacaggag tcgtcgcgcc
    20761 aatgttgtta aattgcttcg ccgcgttttc catcagatca acagtaaaat tggttgattc
    20821 agcggccaca ctacagccgc tatctctgac atagccgcgg atagtaatcg tgctatccgc
    20881 agccaaagcg cgactcaccg ccagccacaa aaaagcgcac agaagataaa aaggtttgtt
    20941 tctcatcacg cccccttaac gacattcagc tgatagctgg gttaataact gctgctgact
    21001 ctctggtggc agttgataat tggcgacaca gtgagcattt tcctcttctc cccatttcac
    21061 ctgaactttt cccgctaaag gcattccgct gaggtaaacc tgaccattat ccgcaacaat
    21121 gccactactc tggctactct ctgatgtcac catcgcccca aacggcagcg gcttattatt
    21181 gtgggttagc gtcatgagca gttttatccc aacgcgcgct ttaaactctg ctcgcacgat
    21241 cgccccacgg gtgggaacaa cgttagcgac cgcattatct aaatcgacgt tatcagccag
    21301 ggtattggta tccagcgcca ctctgttttc ccgatattca gtggcatatg gcagcacggc
    21361 ataaccccgc cagtcggtac gcactcctgt ctggttttcg acttttgcat cttttgcgcc
    21421 aggcgcttta acaagcacca ccgtatcgtt taacggctgc cccagcgtta cgccattggc
    21481 atgagccagt accccaccgc tgactccgta atagagctgc ttaatatcat cgctatggct
    21541 gtaaccgata ttggcattgc cgtaaccacc gcgataattc agcgtggcgt agcctgtact
    21601 tccgctattt ccatcgcctc ccccggcata accggtttgc acgctatagc tgaggttgtt
    21661 gtcttccagc aacgtaccgt atacaccggc cagattggtc atccgaccgt tgagatcgtg
    21721 tgacatgctg tagctggcac tggcatgtcg ccactgagat ttactgtcag aacgcagcca
    21781 gtggctgaaa ggaatattga cgttaagcgc taacatctga tcccgtcctt tttgccaggc
    21841 gtttttcgtc aggctatagc tgagcgtcca gttaatatct tcgaacgcag tatttaatcc
    21901 agcctggaat tgctcatcga cattactcgt tccccaataa gtttgatggc taccactcaa
    21961 atacagtgtt gatgtgcgcc cgagttgctg agtaacggtg agttgtaatt tcccgcgttt
    22021 gttataagcg agattgtaat agtcggtgaa tttcggctta acctgaataa ctccatcctg
    22081 tgtttcgatg ttgtagccat tcattcgact gtatgttgta tcagcgaaat taaaatatcc
    22141 actggtcgaa taacggtaac ccactaactg aatattcgtg cctgactcat tgagcgattt
    22201 gttataaaga aaacgcaccg attgtccgtc atgctgactg tcatcgggaa gtgtggaatt
    22261 agcctgcgtc atatccacag acagagcgcc cagtgccccc atgtttttcc cgataccgaa
    22321 attaaacgca cgataacgat ccgcaagttg cgttccacca tatattgtcc agccagccgg
    22381 aaggccgtgg agtaatgtac tctggaaaaa gcggggtttt tcctgttgcg catttccact
    22441 acggtattct cctgccgtaa tggaataacg tgtatgccct tcacgttgca agagcgggac
    22501 tgacgaatag ggtacggtaa aaatctgcgt actgccgtca gcctctttaa tcgttacctg
    22561 caagtcacca ctattacctg cggcatagat atcgttgatg gtaaaaggcc ccggtggcac
    22621 cgtactatta taaatgtcat acccattttg tttaatagtg acctgtgcag taccacgagc
    22681 aataccgtgg atcaccgggg caaatcctct ttggctatcg ggtaacatat tgtcatctga
    22741 ggccaattgt gcgccgcgaa agttaatacc atcgaaaata tcgccctgag tataaccatc
    22801 acccagcgtc agccgggaac gtaacggtat tatgtctcgc tcaagccagg tattgatatg
    22861 ctgccattta tttttgctac ctgatgaact gtcgctactg ttataactcc aggtggtatt
    22921 gtcgcgtaaa cgccacgcac caatatttaa cccactctgt aggtttaaat atgcataatg
    22981 gctgttaccc ccaattcgat tctgtacact atttccgctg aaattataat tgagcaatcc
    23041 ggcattaata ccgggatccc ataactcagg aggaatataa ccacgcgcgc gattactcat
    23101 aaatgcctga gggatcgtca ggttgagtcg ctgctgaccc acatccagtt gcgcagtagc
    23161 gtcatggatc attgaggtta atggcacgca ggcatcatcc gccagcagat tcataccgga
    23221 gacagaagcc gtattcagcc ccatactggc aagttgcgcg cgtgtcaggc agggaacaat
    23281 cccttgttcg ctgtcgcccg tattaaatgt gacatcacgc gttgccatat aaccattatt
    23341 caaatagata tcaacacgat acgtccctgg cggtaattct tgcccatttt caaaacgcga
    23401 taaatcagcc acagcctggg gatcatccgc taaaaaacgc gggttaaaat agagttcggc
    23461 agatgacaaa ggtgcctgtg cggtaaaagc acaggcaaca aagagtcgga caaaaaaacc
    23521 agccaaacga tgcctacgaa tatgcaagca ttgtgtgttt cgctggtaaa gtcttaaatt
    23581 cagatatgac attattcctc cggtttaaat cacccggcag tcaattcttt ttattcaggc
    23641 gaaaaaatcc ccctgcgtta ttccattacg cccgtcattt tgggggtaag tgcgccataa
    23701 tcatttattg ttcggtaagt aatattgctt cctgcatcag aaggcaattt aaccgtgctt
    23761 tcgcccattg gaggcaccaa tgcattttca agaacccgag ttccggcatt caactctgtt
    23821 accgtcaggt aatagggtgt cgggttaatc agcgtcagag aattcgcgct acgacgaaat
    23881 cttaattttt ctgcggcctg atcgggtggc aacgctaatt tagccggacg atagtacagt
    23941 ttaatgcggc tgataattgc gagctgtagc gtattctcag tcaattttga tttatccatt
    24001 gacggaatcg ctttaacatt catccagaat aaactttccc gatcctgtgg taattggtta
    24061 tttgttgcat caagaatacg caaggtattc tctttttttc ctttcatcgc aaacagaggt
    24121 ggcgtcacga taaaacggcc atcctttaca ccatcggcat tttctaccca tgattgaatt
    24181 aaataggtgc tattttcatc attatttgtc acggcaagtt gcacttgttt ttgccctgcc
    24241 ggataaatta cgcgagtcgc acctaaggcc actcccgctt cagcgcgtcc ggcaaccacc
    24301 attgccatga acatcaggat acctgccagc aagcagaatg ttatttcctg cgatttcctt
    24361 acattgacgt ttttattact cactgtcctg ttcctgttat cacattatct gctgaataat
    24421 tactgatagg ttaaagagaa ccaggcctgg gcattggcga tgccgccagt aacccgacgc
    24481 ccggtagcac gatatttggc gatgaaatgt agcgacgtag agcctgagta aagccgtttc
    24541 cagtttactg gaggacgatt aatcggtacg aggtttcctt catcatcaaa caacgctacg
    24601 ccaatattgg tggctatccc tggcccctct cccacggaaa gcacatccgg atttttacca
    24661 tccgcgacac cgtgaaacgc cacccccaca cgttcactca ccaccgtgct acattcccgt
    24721 aaatgaataa caaaaggcac tggtgcgcta tcttccccta ccgcatgaaa ccggttactg
    24781 ctgatttgcc ccatattgac cgtcatttgt ttatcgccgg cttcaatccg gcaagtttcc
    24841 gcaataatga cgccctgaaa ttgcatattt ccgccaggca gcgtggtatt ccatttattt
    24901 ccggccagag caaacattgg cagcaacgac gctaacagaa ataatctctt ccttttcatc
    24961 gttattttta tcgcacaagg gtgggcatcc ctgcccgtaa tgacgtccct gaacctggta
    25021 ggttattgat actgaacctt gaaggtcgca tccgcattag cagcacccgg ggttgcctcg
    25081 ccgattgcat aataacgcgc ctggaacgga atggtgttgg taccgttatt cagggttgtt
    25141 tgtgcactaa atgtcgcacc gtccagcgcc agcgcagcac ccgttctgtc cagaatctgc
    25201 acaccaactt ttgttgcact acccgcagct gaactctgca aagctaatac gttaggatga
    25261 gttccatcaa ttgcggtacc aaggaaggca atagctgctt tgtctgcaac agtagtgtca
    25321 caatcgttca gttgaatgtt aaaaccgaca gcagaactgg ttgctccttc ctgcgccagc
    25381 gatgcggtac gaacctgacc taactgaacg gtttgatcaa cagagcctgc atcaactgcg
    25441 caagcggcgt taacaacttc ccctttaaag tgaacggtcc caccattaac cgtcgtggca
    25501 gcggccagag ccgctgtgga actgagggac agagccgaca gaacaacaat tgccagagtt
    25561 ttaattttca tactgctttc ctttaaaaaa actatttcta aatcgacatg ggcagtcgtt
    25621 ctgtacactt tgttttgtca acgagtttga ctgccaacac tgcacagttt tcccccaaaa
    25681 gatgaaacat ttggggccat tttgactcat agaggaaagc atcgcgcaca aactttttca
    25741 gtttatttgt tggcttaatg ttctatagtt atctttattt gcagtttttt atattgcatg
    25801 aggtggtttt tggagagaag aatgaggaag ttgcgtcgag ctacagaaac gttagcttta
    25861 catatagcgg aggtgatgtg aaattaattt acaagagaaa taatttacat atcaaacagt
    25921 tagatgattt ttgtcgtttt ttaatatttt
//
LOCUS       DAZFFE010000067        25828 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFFE010000067 DAZFFE010000000
VERSION     DAZFFE010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.067"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(281..2854)
                     /gene="clpB"
                     /locus_tag="ACHJ69_002927"
     CDS             complement(281..2854)
                     /gene="clpB"
                     /locus_tag="ACHJ69_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO5015871.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEDEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HYRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2984..3715)
                     /gene="yfiH"
                     /locus_tag="ACHJ69_002928"
     CDS             complement(2984..3715)
                     /gene="yfiH"
                     /locus_tag="ACHJ69_002928"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO5015872.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRTGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASTAFIQHGDKYLADIYQLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3712..4692)
                     /gene="rluD"
                     /locus_tag="ACHJ69_002929"
     CDS             complement(3712..4692)
                     /gene="rluD"
                     /locus_tag="ACHJ69_002929"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO5015873.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEEMRADFEEHKDEVDWL"
     gene            4827..5564
                     /gene="bamD"
                     /locus_tag="ACHJ69_002930"
     CDS             4827..5564
                     /gene="bamD"
                     /locus_tag="ACHJ69_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO5015874.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5834..6175
                     /gene="raiA"
                     /locus_tag="ACHJ69_002931"
     CDS             5834..6175
                     /gene="raiA"
                     /locus_tag="ACHJ69_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO5015875.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6278..6403
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6279..6326
                     /gene="pheL"
                     /locus_tag="ACHJ69_002932"
     CDS             6279..6326
                     /gene="pheL"
                     /locus_tag="ACHJ69_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO5015876.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6425..7585
                     /gene="pheA"
                     /locus_tag="ACHJ69_002933"
     CDS             6425..7585
                     /gene="pheA"
                     /locus_tag="ACHJ69_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO5015877.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSAINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHSLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7628..8749)
                     /gene="tyrA"
                     /locus_tag="ACHJ69_002934"
     CDS             complement(7628..8749)
                     /gene="tyrA"
                     /locus_tag="ACHJ69_002934"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO5015878.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVSDAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYSQRFQSESRVLLRQANDNRQ"
     gene            complement(8760..9830)
                     /gene="aroF"
                     /locus_tag="ACHJ69_002935"
     CDS             complement(8760..9830)
                     /gene="aroF"
                     /locus_tag="ACHJ69_002935"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO5015879.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            10040..10405
                     /gene="yfiL"
                     /locus_tag="ACHJ69_002936"
     CDS             10040..10405
                     /gene="yfiL"
                     /locus_tag="ACHJ69_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO5015880.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDTFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWRKGADENASTIRLN"
     gene            10553..11071
                     /gene="yfiR"
                     /locus_tag="ACHJ69_002937"
     CDS             10553..11071
                     /gene="yfiR"
                     /locus_tag="ACHJ69_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO5015881.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPGLSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAIISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            11061..12287
                     /gene="dgcN"
                     /locus_tag="ACHJ69_002938"
                     /pseudo
     CDS             11061..12287
                     /gene="dgcN"
                     /locus_tag="ACHJ69_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            12303..12785
                     /gene="yfiB"
                     /locus_tag="ACHJ69_002939"
     CDS             12303..12785
                     /gene="yfiB"
                     /locus_tag="ACHJ69_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO5015882.1"
                     /translation="MIKHLLAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     VLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12862..13209)
                     /gene="rplS"
                     /locus_tag="ACHJ69_002940"
     CDS             complement(12862..13209)
                     /gene="rplS"
                     /locus_tag="ACHJ69_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO5015883.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13251..14018)
                     /gene="trmD"
                     /locus_tag="ACHJ69_002941"
     CDS             complement(13251..14018)
                     /gene="trmD"
                     /locus_tag="ACHJ69_002941"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO5015884.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(14049..14597)
                     /gene="rimM"
                     /locus_tag="ACHJ69_002942"
     CDS             complement(14049..14597)
                     /gene="rimM"
                     /locus_tag="ACHJ69_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO5015885.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14616..14864)
                     /gene="rpsP"
                     /locus_tag="ACHJ69_002943"
     CDS             complement(14616..14864)
                     /gene="rpsP"
                     /locus_tag="ACHJ69_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO5015886.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(15001..16362)
                     /gene="ffh"
                     /locus_tag="ACHJ69_002944"
     CDS             complement(15001..16362)
                     /gene="ffh"
                     /locus_tag="ACHJ69_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO5015887.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16529..17320
                     /gene="ypjD"
                     /locus_tag="ACHJ69_002945"
     CDS             16529..17320
                     /gene="ypjD"
                     /locus_tag="ACHJ69_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO5015888.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIFQQLIS"
     gene            17341..18627
                     /gene="yfjD"
                     /locus_tag="ACHJ69_002946"
     CDS             17341..18627
                     /gene="yfjD"
                     /locus_tag="ACHJ69_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO5015889.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18682..19275)
                     /gene="grpE"
                     /locus_tag="ACHJ69_002947"
     CDS             complement(18682..19275)
                     /gene="grpE"
                     /locus_tag="ACHJ69_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO5015890.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19398..20276
                     /gene="nadK"
                     /locus_tag="ACHJ69_002948"
     CDS             19398..20276
                     /gene="nadK"
                     /locus_tag="ACHJ69_002948"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO5015891.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQQADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRFDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20362..22023
                     /gene="recN"
                     /locus_tag="ACHJ69_002949"
     CDS             20362..22023
                     /gene="recN"
                     /locus_tag="ACHJ69_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO5015892.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSQLQEMT
                     ARYHLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYANELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22172..22513
                     /gene="bamE"
                     /locus_tag="ACHJ69_002950"
     CDS             22172..22513
                     /gene="bamE"
                     /locus_tag="ACHJ69_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO5015893.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22575..22865)
                     /gene="yfjF"
                     /locus_tag="ACHJ69_002951"
     CDS             complement(22575..22865)
                     /gene="yfjF"
                     /locus_tag="ACHJ69_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO5015894.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDIVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22855..23292)
                     /gene="ratA"
                     /locus_tag="ACHJ69_002952"
     CDS             complement(22855..23292)
                     /gene="ratA"
                     /locus_tag="ACHJ69_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO5015895.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMSLVDGPFKKLIGGWKFTPLSQDACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            23463..23945
                     /gene="smpB"
                     /locus_tag="ACHJ69_002953"
     CDS             23463..23945
                     /gene="smpB"
                     /locus_tag="ACHJ69_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO5015896.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24159..24521
                     /gene="ssrA"
                     /locus_tag="ACHJ69_002954"
     tmRNA           24159..24521
                     /gene="ssrA"
                     /locus_tag="ACHJ69_002954"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            24687..>25828
                     /locus_tag="ACHJ69_002955"
     CDS             24687..>25828
                     /locus_tag="ACHJ69_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HFO5015897.1"
                     /translation="MAKIAKKLTDTEIKNTKPAEKEINLFDGDGLMLRIAPLSVGGKK
                     KWYFRYAVPVTKKRTKMSLGTYPHLTLAKARALRDEYLSLLANGIDPQIHNNDKANAL
                     KDATEHTLQAVAKKWLDEKVKTSGISQDHAEDIWRSLERNIFPGLGNLPIKEIRPKLL
                     KQHLDPIEQRGVLETLRRIISRLNEIFRWAATEELIEFNPADNLGQRFSKPKKQNMPA
                     LPPDDLPRFLIALSSASIRLETRLLIEWQLLTWVRPGEAVRARWSDIDQDHRFWNIPG
                     EFMKMKRPHKIPLSKEAMRILELIRPISGHREWVFPSIKAPLNHMHEQTANAAIIRMG
                     FGGELVAHGMRSIARTAAEESGKFRTEVLESALAHTKNNEIIAAYNR"
BASE COUNT         6619 a         6769 c         6044 g         6396 t
ORIGIN      
        1 ctcagcggcg cgggctgtgc ataatacgct ttcccgctac agagtcaagc atttattttt
       61 gctttctctg ccgaagttct caggagaacc ccgctgaccc agcggcgtgt ttgccgttgt
      121 tccgtgtcag tggtggcgca ttatagggag ttattccggc ctgacaagca aaaaatataa
      181 aaactttatt gttcgctcat ttttcaggca aaacatcttt aatatagtct tttccgtcta
      241 acttatagac aaaaacgagc cccgaaggct cgttttatca ttactggacg gcgacgatcc
      301 ggtcttcatt aacttccagg cgaataactt tacccggaac caattcacca gacagtattt
      361 gctgtgccag cgggttttcg atctgctgct ggatagcacg cttcagagga cgtgcaccat
      421 agaccggatc gtaaccgttc tcgctcagca gtttcagcgc ctcgtcagaa atgtggattt
      481 cataaccacg ttcttccaga cgtttgtaca gacgtttcaa ctgaatctgc gcaatcgacg
      541 caatgtgttg ttcacccagc ggatggaaga cgacgacttc atcgatacgg ttaatgaatt
      601 ccggacggaa gttatggctt accacaccga gcaccagctc tttcatgtgc gcataatcca
      661 gttcaccaaa acgttcctga atcagatcag aaccgaggtt agaggtcata atgacgaccg
      721 tattacggaa gtcgaccgtt ctcccctgcc cgtcagtcag acgcccatca tccagtacct
      781 gcaacagaat gttgaagaca tccggatgcg ctttttccac ttcatccagc aggatgacgg
      841 aatacggacg acgacgcacc gcttcggtca gatagccacc ttcttcataa ccaacatatc
      901 ccggaggcgc accaaccaga cgagacaccg agtgtttctc cataaactcg gacatatcga
      961 tacggaccat cgcctcgtcg ctatcaaaca taaagttcgc cagcgcctta caaagctctg
     1021 ttttccccac acccgttggg ccgaggaaca ggaatgaacc aatcgggcga tttggatccg
     1081 ccagccccgc acggctacga cgaatagcgt tagataccgc atcaaccgct tcgttctgac
     1141 caataacgcg atagtgcagt tcttgctcca tacgcagcag tttttcgcgc tcgctttcca
     1201 tcatgcgaga aaccggaatt cccgtccaac gcgccagcac ttcagcaatt tcggcatcgg
     1261 tcactttatt acgcaacaga cgcatagttt tgccttcgag ctgcgttgcg gcttccagtt
     1321 gcttttccag ttccgggatt ttgccgtatt gcagttcaga catccgcgcc aggtcaccca
     1381 cacggcgagc ctgttcaata gcgattttcg cctgttccag ttccgcttta atggtctgcg
     1441 taccagaaag cgaggctttc tctgctttcc actcatcttc taactcggag tactgacgtt
     1501 ctttgtcgct cagttcttcg ttgagcatat ccaggcgttt tttactggct tcatcagact
     1561 ctttcattaa cgcctgttgt tccagtttga gctggatgat acgacgatcg agtcggtcga
     1621 gttcttctgg ttttgagtca atctgcatac gaatgctgga tgctgcttca tcgatcaggt
     1681 cgatggcttt atccggcagc tgacggtcag caatgtagcg atgagacaac gtcgctgctg
     1741 caacaattgc cgggtcagta atttgcacat ggtggtgcag ctcgtaacgt tctttcaggc
     1801 cacgcagaat agcaatggta tcttcaacag aaggctcggc aacaaacact ttctggaaac
     1861 gacgttccag cgcagcatct ttttcgatgt actggcgata ttcgtcaagc gtcgtggcac
     1921 ctacgcagtg caattcacca cgcgccagtg caggtttcag catgtttccg gcgtccattg
     1981 cgccatcggc tttacccgcg ccgaccatgg tatgtaattc gtcgataaac aggatgacgt
     2041 tgccttcctg tttagcaaga tcgttaagta cgccttttaa acgttcttca aactcaccgc
     2101 gatatttcgc cccagccacc agcgcgccca tatccagcgc cagtacgcgg cggcctttca
     2161 acccttcggg cacttcgccg ttgataatac gctgcgccag accttcaacg atggcagttt
     2221 taccgacgcc gggttcacca atcaataccg ggttattttt agtacgacgt tgcagcacct
     2281 gaatggtacg gcgaatttct tcatcacgac caatcaccgg atcgagtttg ccttgttcgg
     2341 ctcgttcggt aaggtcgatg gtatattttt tcaaagcctg acgttggtct tcagcacctt
     2401 gatcgttcac gctttcacct ccacgcattt gttcaatcgc ttgagtaatg ttggcggtgg
     2461 tcgcccctgc tgctttcagg atgtcagcca gcgtgccgcg agactcaagt gccgccagaa
     2521 cgaacagttc tgacgagata aagttatcac cacgtttttg cgccagcttg tcgcaaagat
     2581 taagaacgcg caccagatcc tgtgatggct ggacatcacc accagtacct tcaacctgcg
     2641 gtaaacgatt taatgcctga ttgatatctg tgcgcaactg gccagcattt atgccagcgg
     2701 atgttaataa aggactaacc gaaccccctt cctgattcag cagggcgctc attaaatgaa
     2761 gtggttcgat gaattggttg tcgtgcccga gtgcaagtga ttgggcatcg gcaagagcaa
     2821 gctggaattt attagtaaga cgatccagac gcataactcc tcccataacg gatcaaagtt
     2881 gctactggag attaaatgag gtcatccctc aattattcaa ggttattgac cagattaatg
     2941 tgaaaagaaa atcacgcgta ccggatcgtc ttgattcttt aggttatatc agccaaatga
     3001 aacttgccat acgaccggtg gtcttgtcgc gacgataaga gaagaaagtc tcattttccg
     3061 tatatgtaca acggtcgccg ccgaaaattt gttcaacacc aacgctcgcc agacgctgcc
     3121 gggcaagctg ataaatatcc gccagatact tatcaccatg ctgaatgaaa gctgtactcg
     3181 ctttggcgtc tactgccata aacgcctcgc gaacctccgc ccccacttca aacgcgcgtg
     3241 gaccaattgc cggccctaac caggcgagaa tattttccgg attatcagca aaacaggaaa
     3301 ccgtctcttc cagcacgcct gcgcacagtc cacgccagcc agcatgagcg gcggcgactt
     3361 ccgttcccgt tcgattgcaa aacagcacag ggaggcaatc ggcagtcatc actgcgcaaa
     3421 ccgtgccggg cgtattgcta taagaggcat ctgctcgttt tgaggcataa ggttcaccag
     3481 taagcttaag cacatctttg ccgtgtacct gctcaagcca gaccggttta gaaggcaaat
     3541 tgcccgcagc aaaaagtcgc ttgcgattct cctcaacgtg atccgggtta tcaccacaat
     3601 gagcgccaag gttgagagag tcgtaaggcg gcaagctcac gccgccgata cgggtagagc
     3661 tacaggccgc aacacctttt ggcagcggcc actgcgggac aatcagctta ctcataacca
     3721 gtccacttca tccttatgct cttcgaaatc ggcgcgcatc tcctcaatca gctccaccat
     3781 atcttgtgga ataggcgcat gccattccat ttcgataccg gagatcgggt gataaagacg
     3841 cagcatggtt gcatgaagcg cctggcggtc aaacttacgc agcgtggaga taaacgcttc
     3901 cgaagcacct tttggcggac gcggacggcc accataaacc ggatcgccca ccagcggatg
     3961 agtgatatgg gccatatgca cgcggatctg gtgcgtacgt ccagtttcca gacgcaaccg
     4021 cagacgcgtg tgcacacgga agtgttccat gatgcgatag tgagtgaccg ccggtttacc
     4081 catcggatgc accgccatgt gagtacgttt ggtcgggtgg cggctgattg gttcatctac
     4141 tgtaccgcca gcggtcatat gaccaattgc caccgcttca tactcacgag taatttcacg
     4201 ccgttgcaaa gattcgacta aacgcgtctg agccggaacg gtttttgcca caaccatcag
     4261 gccggtggtg tctttatcca gacgatggac gatgcccgca cgcggtacat cggcaatggg
     4321 cggatagtaa tgaagcaacg cattcagtac cgtaccatcc gggttacccg cgccaggatg
     4381 taccaccagg tcgcgtggtt tattaatgac gatgatatct tcatcttcat agacgatatc
     4441 cagcgggata tcctgcggtt caaaacgcgc ttcttcttca atctcagcgt tgatggccac
     4501 ctgctcgcca cccaatactt tttctttcgg cttatcacaa actttgccgt taaccagcac
     4561 gcgctggtcg aggatccatt cttttattcg cgaacgtgaa taatccggga acatttcggc
     4621 caaagcctga tctaagcgtt gaccgagttg gttttcggac accgttgcag tgagctgtac
     4681 tcgttgtgcc atatatactg cttcttcgtt tatcgttggg gttttacggc tttgccgttt
     4741 aatatagtgt gctattgtag ctggtcttaa ccgggagcag gaacagagaa tctcccgtat
     4801 tacattttga ggaaagtcaa aacgtcatga cgcgcatgaa atatctggtg gcagccgcca
     4861 cactaagcct gtttttggcg ggttgctcgg ggtcaaagga agaagtacct gataatccgc
     4921 caaatgaaat ttacgcgact gcacaacaaa agctgcagga cggtaactgg agacaggcaa
     4981 taacgcaact ggaagcgtta gataatcgct atccgtttgg tccgtattcg cagcaggtgc
     5041 agctggatct catctacgcc tactataaaa acgccgattt gccgttagca caggctgcca
     5101 tcgatcgttt tattcgcctt aacccgaccc atccgaatat cgattatgtc atgtacatgc
     5161 gtggcctgac caatatggcg ctggatgaca gtgcgctgca agggttcttt ggcgttgacc
     5221 gtagcgatcg cgatcctcaa catgcacgag ctgcgtttag tgacttttcc aaactggtgc
     5281 gcggctatcc gaacagtcag tacaccaccg atgccaccaa acgtctggta ttcctgaaag
     5341 atcgtctggc gaaatatgaa tactccgtgg ccgagtacta tacagaacgt ggcgcatggg
     5401 ttgccgtcgt taaccgcgta gaaggcatgt tgcgcgacta cccggatacc caagctacgc
     5461 gtgatgcgct gccgctgatg gaaaatgcat accgtcagat gcagatgaat gcgcaagctg
     5521 aaaaagtagc gaaaatcatc gccgcaaaca gcagcaatac ataacagaaa cctgaaacac
     5581 aaaacggcag ccttgagctg ccgttttttt attctgttag ttgtgaaact gaagcgattt
     5641 agtcactatc gatctcatca aatatggctc gctttgagat attcctcaag taaaaaaaca
     5701 cctcttcctg cgatttctca caaaaaagat tcgttgacaa aaagtgacaa aattatgaga
     5761 tttccatcac acattttaac atcaggaacg ttatgctgaa ttcaccaaga cgggaagaca
     5821 agaggtaaaa tttatgacaa tgaacattac cagcaaacaa atggaaatta ctccggccat
     5881 ccgccaacat gtcgcagacc gtctcgccaa actggaaaaa tggcaaacac atctgattaa
     5941 tccacatatc attctgtcca aagagccaca agggtttgtt gctgacgcca caatcaatac
     6001 acctaacggc gttctggttg ccagtggtaa acatgaagat atgtacaccg caattaacga
     6061 attgatcaac aagctggaac ggcagctcaa taaactgcag cacaaaggcg aagcacgtcg
     6121 tgccgcaaca tcggtgaaag acgccaactt cgtcgaagaa gttgaagaag agtagtcctt
     6181 tatattgagt gtatcgccaa cgcgccttcg ggcgcgtttt ttattgacag cgtgaaaaca
     6241 gtacgggtac tgtactaaag tcacttaagg aaacaaacat gaaacacaca ccgtttttct
     6301 tcgcattctt ttttaccttc ccctgaatgg gaggcgtttc gtcgtgtgaa acagaatgcg
     6361 aagacgaaca ataaggcctc ccaaatcggg gggccttttt tattgataac aaaaaggcaa
     6421 cactatgaca tcggaaaacc cgttactggc gctgcgagag aaaatcagcg cgctggatga
     6481 aaaattatta gcattactgg cagagcgacg cgaactggcc gtcgaggtgg gaaaagccaa
     6541 actgctctcg catcgcccgg tacgtgatat tgatcgtgaa cgcgatttgc tggaaagatt
     6601 aattacgctc ggtaaagcgc accatctgga cgcccattac attactcgcc tgttccagct
     6661 catcattgaa gattccgtat taactcagca ggctttgctc cagcaacatc tcaataaaat
     6721 taatccgcac tcagcacgca tcgcttttct cggccccaaa ggttcttatt cccatcttgc
     6781 ggcgcgccag tatgctgccc gtcactttga gcaattcatt gaaagtggct gcgccaaatt
     6841 tgccgatatt tttaatcagg tggaaaccgg ccaggccgac tatgccgtcg taccgattga
     6901 aaataccagc tccggtgcca taaacgacgt ttacgatctg ctgcaacata ccagtttgtc
     6961 gattgttggc gagatgacgt taactatcga ccattgtttg ttggtctccg gcactactga
     7021 tttatccgcc atcaatacgg tctacagcca tccgcagcca ttccagcaat gcagcaaatt
     7081 ccttaatcgt tatccgcact ggaagattga atataccgaa agtacgtctg cggcaatgga
     7141 aaaggttgca caggcaaaat caccgcatgt tgctgcgttg ggaagcgaag ctggcggcac
     7201 tttgtacggt ttgcaggtac tggagcgtat tgaagcgaat cagcgacaaa acttcacccg
     7261 atttgtggtg ttggcacgta aagccattaa cgtttctgac caggttccgg cgaaaacgac
     7321 gttgttaatg gcgaccggac aacaagctgg tgcactggtt gaagcgttgc tggtactgcg
     7381 caaccacagt ctgattatga cccgtctgga atcacgtccg attcacggta atccgtggga
     7441 agagatgttt tatctggata ttcaggccaa tcttgaatca gcggaaatgc aaaaagcatt
     7501 gaaagagtta ggggaaatca cccgttcgat gaaggtattg ggctgttacc caagtgagaa
     7561 cgtagtgcct gttgatccaa cctgatgaaa aagtgccgga tgatgtgaat catccggcac
     7621 tggattatta ctggcgattg tcattcgcct gacgcaataa cacgcggctt tcactctgaa
     7681 aacgctgtga gtaatcgccg aaccagtgct ccaccttgcg gaaactgtca ataaacgcct
     7741 gcttatcgcc ctgctccagc aactcaatcg cctcgccgaa acgcttatag taacgtttga
     7801 ttaacgccag attacgctct gacgacataa taatgtcggc ataaagctgc ggatcctgag
     7861 caaacagtcg cccgaccatc gccagctcaa ggcggtaaat cggcgaagag agcgccagta
     7921 gttgctcaag ctgaacattt tcttctgcca ggtgcagccc ataagcaaaa gtagcaaagt
     7981 ggcgcagagc ctgaataaac gccatattct gatcgtgctc gacagcgcta atacgatgca
     8041 accgagcgcc ccagacctga atttgctcca gaaaccattg gtatgcttcc ggcttacgtc
     8101 catcacacca gaccacaact tgctttgcca ggctaccgct gtccgggccg aacatcgggt
     8161 gtaaccccag caccgggcca tcgtgcgccg ccagcatggc ctgtaatggt ccatttttca
     8221 ccgatgccag atcaaccaga atacaatctt tcggtaaagg cggtaatttg ccgataactt
     8281 gctcagtaac gtggatgggc acactaacaa tcaccattcc ggcatcggaa acaatatcag
     8341 ccgctcgatc ccagtcatgt tgctccagaa tccgcacctg ataacccgag agtgtcagca
     8401 tcttctcgaa cagacgtccc atctgaccgc caccaccgac gataaccacc gggcgcagtg
     8461 acggacaaag cgttttaaat cctttgtcgt tttcactgga gtaagattca cgcatcaccc
     8521 gacgcaaaac atcctcaatc agatctggcg gtacacccag agcttccgcc tctgcacgac
     8581 gcgaggccaa catagatgcc tcgcgctccg gaacataaat aggcagtcca aagcggcttt
     8641 tcacctcgcc cacttcagca accagttcca gacgcttcgc taataaattc agcagcgctt
     8701 tatcgacttc atcaatttga tcgcgtaatg cggtcaattc agcaaccata ataaacctct
     8761 taagccacgc gagccgtcag ctgcccgttc agatcctgat gaatttcacg cagcaaggca
     8821 tcggtcattt cccagctaat gcaggcatcg gttacggata caccgtattt catttcactg
     8881 cgcggttgct cggaagactg attgccctcg tggatattac tttcgatcat cagaccaata
     8941 attgagcgat tgccatcttt gatttgagca accacggatt ctgccaccgc aggctgacgg
     9001 cgataatctt tattggaatt accgtggctg caatctacca tcagagacgg gcgcagtccc
     9061 gcctgttcca tctctttttc acattgcgca acatccgcag ggctatagtt tggcgcttta
     9121 ccaccgcgca ggatcacatg cccgtccgga ttcccctgag tttgtagcaa cgcaacctgc
     9181 cctgcctggt taatgccaac aaaacggtgc ggctgggcgg cggcacgcat agcgttaatt
     9241 gccgttgcca gactgccatc ggtgccgttt ttaaaaccaa ccggcatgga aagcccggag
     9301 gccatttcac ggtgagtttg cgattccgtt gtacgagcac caattgctga ccagctaaac
     9361 agatcgccca agtattgcgg gctattcgga tctaacgctt ccgtcgccag tggcagtccc
     9421 atattcacca gctcaagcag caatttacgc gcgatctgca gcccggcttc tacatcaaaa
     9481 gagccatcca tatggggatc gttaattaac cctttccagc cgacagtggt acggggtttt
     9541 tcaaaataga cgcgcattac cagatagagg ctatcgctga cctctgcggc aagggcttta
     9601 aatcgacgag catattccag agcagtttcc ggatcatgaa tggaacaagg accacatact
     9661 accagcagac gaggatcgcg cccggcgata atatctgaaa tggttttacg cgagtcagca
     9721 atctgggctt cttgttgcag gcttaatgga aaagcggcct tcagttgttc cggagtcatt
     9781 aaaacctgtt cgtcggtaat atgtacgtta ttcagcgcgt ctttctgcat gatggcgatc
     9841 ctgtttatgc tcgtttgcga tagttgatcc tcagcgagga tgacgtaacg ataacacata
     9901 aagtaaagtt ttcaatccat atttcgtaca tatttcttta cataagcaat ttattcgcgc
     9961 tttttaccct tattcatgta tatataaatt tacaccatct ataaaaacaa tccgctatgc
    10021 tttgaaaaag gagaaagaaa tgatgaaaaa atttatcgcc cccttgttgg ctttactggt
    10081 tagcggatgt cagattgatc cttatactca cgcgccaacc ttaaccagca ccgactggta
    10141 tgatgttggt atggaagatg cgatatcggg cagcgccatt aaagatgacg atacatttag
    10201 cgattcacag gcggatcgcg gtctatacct taaaggatat gccgaaggac aaaagaaaac
    10261 ttgccagacc gattttactt atgcccgggg actttctggt aaaagctttc ctgccagctg
    10321 caataatgtt gaaaatgcca gccaactgca tgaagtatgg cgaaaagggg ctgatgaaaa
    10381 cgccagcaca atacgattaa attaattacc cctcaaaatc aattaagcat gagattttac
    10441 tcattatgtt ttatttctca cattgatgac gaccgcatga aaggatgata ttttaatgag
    10501 cattctgaaa aaatatcgat gcatttcgag cgaagatggt gaggatccct gaatgcgttt
    10561 ttctcaccga ctgttcttac tgctgattct cctgctgacg ggagcaccaa tactcgcaca
    10621 ggaaccttct gacgttgcga aaaacgtgcg tatgatggtt tccggtattg tcagttacac
    10681 ccgctggcct gggctatcag ggccaccaaa gttatgtatc ttttcatctt cacgttttag
    10741 taccgcctta caagaaaatg ctgctacatc tttaccttat ctgcctgtca tcattcatac
    10801 tcaacaagag gcgataattt caggctgcaa tggtttttat tttggcaacg agtcaccgac
    10861 attccagatg gaattaaccg aacaatatcc gtcaaaggcg ttattattaa ttgccgaaca
    10921 aaataccgag tgcattattg gtagcgcctt ttgtctgatc atccacaata atgacgtcag
    10981 atttgccgta aacctggatg ccttatcgcg tagcggtgta aaggtcaacc cggatgtctt
    11041 aatgctcgca cggaagaaaa atgatggata acgataattc tcttcataag cgccccacgt
    11101 ttaaaagagc attacgcaac atcagcatga ccagcatatt tatcactatg acgctgatct
    11161 ggctgctgct ttccgtgacg tcagtgctaa ccctgaaaca atacgcgcaa aaaaacctgg
    11221 cactgacagc agcaacaatg acttacagtc tggaagcagc tgtcgttttt gccgatggcc
    11281 ctgcagcaac tgaaacactg gcagcgctgg gccagcaagg ccaattttca actgcagaag
    11341 tgcgtgataa gcagcaaaat attctggcat cctggcatta cacccataag gaaccaggcg
    11401 atactttcag caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc
    11461 gccacaatgg tgaaaccatt ggcgaagtac gcttaactgc tcgcgacagt tcaatcagcc
    11521 attttatctg gttttcgctc gctgtcctga ccggttgtat tctgctggca tcaggcatcg
    11581 caattaccct cacccgccat ttgcacaatg gcctggtgga agcgctgaaa aatatcaccg
    11641 atgtcgtaca tgatgtgcgt tctaaccgca atttttcccg acgagtttcg gaagaacgta
    11701 tcgctgagtt tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt
    11761 ggcagcttcg tttacaggcc aaaaatgcgc agcttctacg taccgcgcta catgacccat
    11821 taaccgggct ggctaaccgc gcagcgtttc gtagcggcat caacacgttg atgaacaatt
    11881 ccgatgcccg aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca
    11941 atgataccta gggtcatgcg acgggcgata gagtcttgat tgaaatagca aaacggttag
    12001 ctgaatttgg cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg
    12061 tgctctatga tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa
    12121 tttttaatct cccgtttgat cttcataatg gccatcagac taccatgaca ttaagcattg
    12181 gttacgcgat gaccattgag cacgcctctg cggaaaattt acaagagctt gccgatcaca
    12241 atatgtatca ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg
    12301 atatgataaa gcacctgcta gcacccctga ttttcacctc gctaatactt actggctgcc
    12361 agtcccctca gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta
    12421 ctgaatccgc cggcgactgg tcgctgggct tatcagatgc cattctgttt gcaaaaaatg
    12481 actacaaatt gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct
    12541 cgacagggct aacacatgcc cgtatggatg gacacaccga caactatggt gaagacagtt
    12601 acaacgaagt cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctatgggtg
    12661 gacaaattcc gcgcagcaat ctcaccactc agggtttagg aaaaaaatat cccatagcca
    12721 gtaacaagac cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc
    12781 cttaaaacac gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca
    12841 ggatgtcgct taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt
    12901 acgctcacgc aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat
    12961 gctgtcaact accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat
    13021 tttacgaaca gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc
    13081 gaatgcctgc agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc
    13141 acccggacgg aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat
    13201 gttgctcata atttaatctc ttatcctggg taaactgata tctcgggggc ttacgccatc
    13261 ccatcatgtt tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct
    13321 tgctcttcag tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc
    13381 agcgactgtt tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc
    13441 ggaacttcca tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct
    13501 tcagcaaagg aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg
    13561 gaaacggagt caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg
    13621 attgaccatt cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga
    13681 ccacacacca gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc
    13741 ttgcgtccct gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct
    13801 gcatgaatgg cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa
    13861 ggacgatcgt ccacggtacg gtgccggtca tgcgtgaagt cacgaggact ccagctctgg
    13921 atgctcagca ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg
    13981 aacatttcag gaaacaggct aattatgcca atccacatag cgccgtcttt taccgtttat
    14041 ccggtggttt aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact
    14101 ttcttgataa cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct
    14161 ttcaggtttg ccttaatgac gagaacgtca ttagatccgg tttccatcat atctacgact
    14221 ttaccgagat cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag
    14281 tagtccccct cttcaagctg aggcaactgc gatgaatcca cgacaatttc acaattcgtc
    14341 agcaggttcg ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg
    14401 tggtgcttcc agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag
    14461 ggctgatagt caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca
    14521 cgaataccgt aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg
    14581 gtgagttgtt tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg
    14641 atcagcgcag caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga
    14701 tccagatcca ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg
    14761 cgctcaatga agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac
    14821 ggacgctttt tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg
    14881 aataaaacaa ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa
    14941 ttttactgat ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat
    15001 ttagcgacca ggaaatcccg gcggcatcat ccctttcatg cttctcatca tcttcgccat
    15061 cccgcccttc ttcattttct tcatcatgcg ctgcatgtcg tcgaactgtt tcagaagacg
    15121 gttaacgtcc tgcacctgca taccgcaacc ggcagcgata cgacgcttac gcgaaccttt
    15181 gatgatttct ggcttagcgc gctctttcat cgtcatcgag ttgatgatgg cttccatacg
    15241 caccagcact ttatcgtcca tctgtgactt gacgttatcc gggatctgcc ccatgcccgg
    15301 cagcttgccc atcaggctag ccatgccgcc catatttttc atctggcgca gctgctcaag
    15361 aaagtcgttg agatcgaagc cgtcgccttt tttcagcttg ctggctaatt tctctgcctg
    15421 cgcgcggtca accttgcttt cgatatcttc gatcagtgac agtacgtcgc ccatgccgag
    15481 aatacgggag gcaatacggt ccggatggaa tggctccagc gcctcggttt tctcgccaac
    15541 accgaggaac ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccacg
    15601 agcatcgccg tcgactttag tcagtaccac accggtcagc ggtaacgctt cattgaatgc
    15661 ttttgccgta ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac
    15721 cggattaatc gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag
    15781 acgaccggcg gtatccacca gcagtacgtc gtagaatttc agtttggctt ctttcagcgc
    15841 cgcgttaacg atatctaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac
    15901 ctgctctgcc agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cggcagaaac
    15961 caccagcact ttcttcttgt gcttctcgcg caggaactta ccgagtttac caacgctggt
    16021 tgttttaccg gcaccttgca ggcccgccat cagcacgacc gcaggcggtt gcgcagccag
    16081 gttcagggtc tggttctctt cgcccatcgc cgcaaccagt tcgttacgga caattttgac
    16141 gaactcctgc cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac
    16201 gcgattgata aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg
    16261 cacttcgcgc agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt
    16321 gcgcagcgtg cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg
    16381 gaaacggttg gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg
    16441 cgtctttgtt gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt
    16501 tcttattgct caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc
    16561 ctactccgtc agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg
    16621 gcgcatggct attatttctg cggtcatcgc gctggtctgc cacgcaatcg ctctggaagc
    16681 ccgcatcctg cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt
    16741 ggtcagtttg atgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt
    16801 gttgctaccc attgtctatg cctttgcgct tatcaacctg gcgctggcga ctttcatgcc
    16861 caatgaatac atcacccatc tggaagcaac gccggggatg ctggtgcaca ttggcttatc
    16921 gcttttttcc tatgccacgc taattatcgc tgccctgtac gcgctgcaac tggcgtggat
    16981 tgattaccaa ctgaagaaca agaagctggc gtttaaccag gaaatgcctc cattgatgag
    17041 tatcgagcgt aaaatgttcc atatcacgca gattggcgtg gtgctgctaa cgctcactct
    17101 ttgcactggc ctgttctaca tgcacaactt atttagcatg gaaaatatcg acaaggccgt
    17161 gctctctatc gtggcgtggt ttgtctatat tgtgctgctg tggggacatt atcatgaagg
    17221 atggcgtgga cgccgcgtcg tctggtttaa cgttgcgggt gcagtcattc tgacactggc
    17281 ctacttcggc agccgaattt ttcagcagtt aatcagctaa acccagaaaa ggagtttccc
    17341 ctggaacaca tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca
    17401 gcctattttt ccgggtccga aaccggaatg atgactctca accgctatcg tcttcgacat
    17461 atggcgaaac agggtaatcg ctcggccaaa cgcgtcgaaa aattactgcg caagccagac
    17521 cgcctgataa gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc
    17581 ggcactattg ttgggatgcg tttgtacggc gatgcgggcg tggcaattgc aactggtgtg
    17641 ctgacttttg tcgtgctggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac
    17701 ccggaaaaag tcgcttatcc gagtagtttt ctactggctc cgctgcaaat tttgatgatg
    17761 ccgctggtct ggttgctgaa tgctattacc cgcatgttaa tgcgcatgat gggcatcaaa
    17821 accgatatcg tggttagcgg ctctttaagc aaagaagagt tgcgcaccat cgtgcacgaa
    17881 tcacgctcac aaatttcccg tcgcaatcag gatatgctgc tgtcggtact cgatctggaa
    17941 aagatgaccg tcgatgacat catggtgccg cgcagtgaaa ttatcggtat tgatatcaac
    18001 gatgactgga aatcgattct gcgccaactc tcccactcac ctcacgggcg catcgtgctc
    18061 taccgtgatt cgctggacga cgccatcagt atgctgcgtg tgcgtgaagc ctggcgactc
    18121 atgtcggaga aaaaagagtt caccaaagaa accatgctac gcgccgcgga cgagatctat
    18181 tttgtcccgg agggtacgcc gctcagcaca cagttggtga agtttcagcg caacaaaaag
    18241 aaagtcggcc tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggtcgaagat
    18301 attctcgaag agattgtcgg cgatttcacc acgtcgatgt cgccaacact tgccgaagag
    18361 gtcacgccgc aaaacgacgg gtcggtgatt atcgatggca ccgccaacgt gcgagaaatt
    18421 aacaaagcct ttaactggca tctaccggaa gatgatgctc gtactgttaa cggcgtcatt
    18481 ctggaagcgc tggaggagat cccggtagca ggcacccgcg tgcgtatagg cgagtacgat
    18541 atcgatattc tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtaaaa
    18601 ccgctgcgcg agagtgtggc ggagtaacga aaacggcccg gcattcgcat gctgggccgt
    18661 gatttattac gaaagcagaa attaagcttt tgctttcgct acagtcacca tcgccgcacg
    18721 aatcgtacga ccgttcagcg tataaccttt ctgcataatg cccagtacgt tacctggcgc
    18781 aacgtcatca gattccacca ttgcgatggc ctgatgcaca tttgggtcca gtgggacgtt
    18841 ggtttcggcg atcacttcaa cgccaaactt acgcacaaca tccagcatcg acttcagcgt
    18901 cagctcaata ccttcaacca tcgcggacat atccgggtta gctttatcag ccacttccag
    18961 cgcacgatcc aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa
    19021 tttgtgagct ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt
    19081 tacgcgcaaa atgccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt
    19141 cgcaattttt tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc
    19201 aatctcttcg tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg
    19261 ttctttacta ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt
    19321 attatgggga tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca
    19381 acaaggacct cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac
    19441 ggcaccccac tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt
    19501 acgaggtcat cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg
    19561 gcacgctcgc ggagattggg caacaggctg atctcgcggt tgtcgttggc ggcgacggta
    19621 atatgctggg cgcggcgcgc acgctcgccc gctacgatat taaagttatt ggaatcaacc
    19681 gtggcaacct gggtttcctg actgaccttg atcccgataa cgcccagcaa caattagccg
    19741 atgtgctgga aggccactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc
    19801 agcaagattg ccagaaacgc atcagcacgg cgattaacga agtggtactt catcctggca
    19861 aagtagcgca tatgattgag ttcgaagtgt atatcgacga gatctttgcg ttttcgcagc
    19921 gttccgatgg actgattatt tcgacgccaa caggctccac cgcatattcc ctctctgcgg
    19981 gcggtcctat tctgacaccc tctctggatg cgattaccct ggtgcccatg ttcccgcata
    20041 cgttgtcagc acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc
    20101 atcgccgtaa cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag
    20161 gtgaagatgt cctgattcgt cgctttgatt accatctgaa tctgattcat ccgaaagatt
    20221 acagttattt caatacatta agcaccaagc tcggctggtc aaaaaagtta ttctaatttt
    20281 acgccagcct ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt
    20341 ttttcataca ggaaaacaac tatgttggca caactgacca tcagcaactt tgctatcgtt
    20401 cgtgagcttg agattgattt tcatagcggc atgaccgtaa taactggcga gaccggcgcg
    20461 ggtaaatcta ttgcaataga tgccctcggc ctttgtctcg gtggtcgcgc tgaagccgac
    20521 atggtgcgta ccggcgctgc tcgtgccgac ctgtgcgccc gtttttctct gaaagatacg
    20581 ccagcggccc tgcgttggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt
    20641 cgtcgcgtaa tcagcagcga tggtcgctcc cgtggtttca tcaacggtac agctgttcct
    20701 ctgtcacaac tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat
    20761 cagttactca ccaaacccga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc
    20821 tctcagctcc aggaaatgac cgcacgttat catttgtggc atcaaagctg ccgtgacctc
    20881 gcgcatcatc aacagttaag tcaggaacgc gccgctcgtg cggagctgct gcaataccaa
    20941 ttaaaagaac ttaacgaatt taatccgcag cctggagagt ttgagcaaat cgacgaagag
    21001 tacaaacgtc tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta
    21061 atggctgacg gtgaagacgc aaacctgcaa agtcaacttt acacggctaa acaactggtg
    21121 agcgaattga ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaggct
    21181 accatccaga ttgctgaagc cagtgatgaa ctgcgccact actgcgatcg tctggatctc
    21241 gatcccaacc gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt
    21301 aaacatcacg tcagccctga ggcattgcca cagtattacc agtcgctact ggaagaacag
    21361 cagcaactgg acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat
    21421 catcagcagg cactggaaac ggcgcgcgcg ttacaccagc aacgccagca ttatgcaaat
    21481 gagttggcac agctgatcac cgatagtatg catgcactct ctatgccaca tgggcagttt
    21541 acgatcgatg ttaaatttga cgagcatcac ctgggcgctg acggcgctga tcgtattgag
    21601 tttcgggtaa ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt
    21661 ggtgagttgt cccgcatcgc attggcaatc caggtcatta ccgcgcgtaa aatggaaacc
    21721 ccggcactga tttttgatga agtggatgta gggattagcg gcccaacagc ggcagttgtc
    21781 ggcaaactgc tacgtcaact tggcgaatca actcaggtga tgtgtgttac ccacctgcca
    21841 caagtcgcgg gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg
    21901 acagaaacgc atatgcaatc cctggataaa aaagcgcggt tacaagagtt ggcgcgcctg
    21961 cttggtggca gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcggcg
    22021 taaacttttt tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc
    22081 aaaaggtttt aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta
    22141 ctgctcgggc ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc
    22201 agcagtacta ttgatgttga ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc
    22261 tgacatcaac caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat
    22321 gacgcaacaa caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac
    22381 aaatacctgg ttctatgtct tccgccagca acctggtcat gaaggtgtaa cgcagcaaac
    22441 gctgacgctg acctttaaca gtagcggtgt gttgaccaac attgataaca aacctgcgct
    22501 gagtggtaac taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac
    22561 cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc
    22621 ggatcggcaa tgagaggacg ataaatctcc acccgatcgc catcatgcac aatatcgctg
    22681 agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc
    22741 aattccagca aaccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcaacgtc
    22801 acgcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc aattttacct
    22861 ggcactgtag acctcttttg cacgaacagt aaaagcctgc accatattag ctgccagctc
    22921 tttaaacaca cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg
    22981 aaactcaata cggcacgcgt cctggctcaa cggcgtaaac ttccaaccgc cgatcaattt
    23041 cttaaacggt ccatcaacca gactcatgag aatactttga ttactggtca actggttgcg
    23101 ggttgtaaac gttttgctga tcccagcctt agacacatct accgcagcag tcatctgccc
    23161 aggagtggac tccagaatcc gacttccggt acaacccggc aaaaactgag gataagactg
    23221 aacgtcattc actaactgat acatttgctc cgcgctgtaa ggcaccagtg cggtccggct
    23281 aatctgaggc ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc
    23341 tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt
    23401 gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgatgc
    23461 ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg
    23521 cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg
    23581 aggttaaatc cctgcgcgca ggaaaagcca atatcagcga cagctatgtc cttctgcgcg
    23641 acggagaggc atttctgttt ggcgctaaca tcacgccaat ggctgtggcc tccacgcatg
    23701 tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat
    23761 tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa
    23821 atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa gaaacagcac gataaacgtt
    23881 cagatatcaa agaacgcgaa tggcaggtgg ataaagcgcg tatcatgaaa aacgcccacc
    23941 gttaaaccta tataagcgat actaatcaaa tcctcaccgc gcctccctat ccggtggcgc
    24001 gaatgaacat cttatcgact gtcaaatctg acacagatgt agccatccca ttgcttaatc
    24061 gaaaaaaatc atactatgca ggcgctaatt ccaggatgat aacgcctttt gggctagtca
    24121 tgctgctcat aaatctggta tacttaccgt tacacattgg ggctgattct ggattcgacg
    24181 ggatttgcga aacccaaggt gcatgccgag gggcggttgg cctcgtaaaa agccgcaaaa
    24241 aatagtcgca aacgacgaaa actacgcttt agcagcttaa taacctgctt agagccctct
    24301 ctccctagcc tccgctctta ggacggggat caagagaggt caaacccaaa agagatcgcg
    24361 tggaagccct gcctggggtt gaagcgttaa aacttaatca ggttagtttg ttagtggcgt
    24421 gtccgtccgc agctggcaag cgaatgtaaa ggctgactaa gcatgtagta ccgaggatgt
    24481 aggaatttcg gacgcgggtt caactcccgc cagctccacc actttttagt tgtttgaagt
    24541 atacttaagt ctactaagcc cgcatggaac cagccttgcg ggctttttta cgtctacaga
    24601 agtctactga gatttgctag aagctactcg ttatggcacc ctttttgaga cccaatgtaa
    24661 agggtcccaa aaccgagggt cctaaaatgg caaagatcgc taagaaactt actgacactg
    24721 aaatcaaaaa taccaagcca gcagaaaagg agatcaacct ttttgatggt gacggcctga
    24781 tgctacgaat cgcccctctc tctgtaggcg gcaagaaaaa atggtatttc aggtatgcag
    24841 tgccagtaac caaaaagcgg accaagatga gcttaggaac ctatcctcat ctcacacttg
    24901 ccaaggctcg ggctttacgt gatgaatact tgtcgttgct tgcaaatggt atcgatccgc
    24961 agattcacaa caacgataaa gccaacgccc ttaaggatgc cactgagcac acgctacagg
    25021 cagttgctaa gaaatggtta gatgagaagg ttaagacctc tggtatctct caagaccacg
    25081 cagaagacat ctggcggagc cttgaacgaa atatcttccc cggtttaggc aaccttccta
    25141 tcaaagagat ccgtcccaag ctcttaaaac aacatcttga ccccatagaa caacgaggcg
    25201 tattggaaac cctgcgccgc atcatttcac gtctaaatga aattttccgt tgggctgcga
    25261 cagaagaact tattgagttt aatcctgctg acaatctcgg ccagagattc agcaaaccca
    25321 agaagcaaaa tatgccagct ttaccaccag atgatttacc tcggttcttg attgctctat
    25381 ctagcgcttc tatacggctc gaaacacgtc ttctaattga gtggcaactg cttacgtggg
    25441 ttcgtccggg agaagctgtc cgcgctcgtt ggagtgatat agaccaagat catcgattct
    25501 ggaacatccc cggtgagttt atgaagatga agcgtcctca caagattccg ctgagtaaag
    25561 aggctatgcg tatacttgag ttaatcagac caatcagtgg tcatagagag tgggttttcc
    25621 caagcattaa ggccccgctt aaccacatgc atgagcaaac agctaatgct gcaatcatcc
    25681 gtatgggatt cggtggtgaa ctggtagccc acggaatgcg ttctatagca agaacggctg
    25741 ctgaagaaag tggtaaattt agaacagagg tgcttgaatc agccttagct catactaaaa
    25801 acaatgaaat catagccgct tacaatcg
//
LOCUS       DAZFFE010000068        25819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFFE010000068 DAZFFE010000000
VERSION     DAZFFE010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.068"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..356
                     /locus_tag="ACHJ69_002956"
     CDS             <1..356
                     /locus_tag="ACHJ69_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015898.1"
                     /translation="QTSVSDFIYYLMEKHNKYPVYVLHIPSNNTIEIGYNIMNENLVI
                     GKGIHFRTLSNRSLYFTLTQPPLIATIKDDVFCTLHDYYNHNNETKSTIDNYISKIQD
                     NHNTPWILNNESVQK"
     gene            complement(1242..1388)
                     /locus_tag="ACHJ69_002957"
     CDS             complement(1242..1388)
                     /locus_tag="ACHJ69_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5015899.1"
                     /translation="MPQKTIIVSILCLTMLLTVWMLHASPCEFRLPCEFRLNIMWSEF
                     AAFL"
     gene            1458..1757
                     /locus_tag="ACHJ69_002958"
     CDS             1458..1757
                     /locus_tag="ACHJ69_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015900.1"
                     /translation="MKNEAEKAMWSCKTSDTPWTFSRLLKKAICAVLPDSGPHTVHRI
                     NKKENNYLTSVLSDICDSNEKDSNEAMFNPFHPLYRDHHPIDSREWSGSPYKTPE"
     gene            complement(1804..3306)
                     /locus_tag="ACHJ69_002959"
     CDS             complement(1804..3306)
                     /locus_tag="ACHJ69_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFO5015901.1"
                     /translation="MTTNNYLEYFLTLLGWLVNNGLWDLLIGTGLFALPLAFKVIGIW
                     LKVREEGADEGNKGMLSLPRIENALYGAFFVMVASCVPLVQVTLDTLKFDRSRAQTCG
                     VWTPKAPGDTGYQGIISSLGDKTAAAPIWWVVVHKLSKGVTQAAVASIPCRPDLRQLR
                     FEVQRTFIANRALADELQDFTNDCYSLAMYQWKQRDQGMTKDRKVLSDISWIGSSTFM
                     QGDYGTLQSRMPRAAFPWNDARDSGRPDTGRGGYPTCREWWNDSSVGLKKRVTEEVDE
                     GLWLRLSAAMKLLGNSQATYQEEIIKRLVSPANLTISQGGEVYRGYGGNADMTTMNAI
                     SRIGATAGSGVASLGAFPAFDAMRQALPMVQSILLMAVYIMIPLVLAFAAYEFSTLIT
                     LTFVVFALNFLTFWWELARWLDSWMITALYGSDTHSSLNMMGLQNTSDDIIMNLVMGT
                     MFLVLPAVWLGALSWAGVNIGQAISEGFNRAGHVTQQAGQHAGSFVKRIK"
     gene            complement(3310..3675)
                     /locus_tag="ACHJ69_002960"
     CDS             complement(3310..3675)
                     /locus_tag="ACHJ69_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015902.1"
                     /translation="MNKTVLMVPGSRAGLLVKGMKLLGKWGLTVIVIMASALLIADTG
                     MRHAQEVDALRLWFHETRFGWLGWRFCIYCGLVWGCRKIWRAPGFRAEYRLRFARICM
                     VSALFFLLCEYAIFSAAGD"
     gene            complement(3668..5098)
                     /locus_tag="ACHJ69_002961"
     CDS             complement(3668..5098)
                     /locus_tag="ACHJ69_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO5015903.1"
                     /translation="MTMKVKTLVMMVMMVMLSCSTPVMADSILSLLKLNDSTFGYGKS
                     VDGAVSDKLFYTLGGGSVISQPATRSNMQKLGLDLGWSSDLMCGNFDLKTTVGNQLNG
                     ITDGFKNLMGDVIQGATGAVSSLPAMVIQRANPGLYDMITNGVLQANVAFDKAQLNCQ
                     NMAKRMMDFSDRSKWTQAAMMEEYKKVVNTSDGDAVKGDDAGRQATGKEGQKWIGGQQ
                     RGGAGQPAIRPVHDMTAAGFNMMNSLPVTSTSGVGAGSCNGSACEKFRNAEEAASAVV
                     KVLGDRSMRTCTDAKECTSGDSDQQPGTAVAGTGFAPMLEEATRINTEQLVRLVNGQD
                     KPTAENLAKLKTGSLAVSAGVIHALRRDPDNMSLTSRLAGELAMADTVETALVMRRML
                     LTGMSEPYAAAQPAALEEGDRRIASLDREIIALKSEMELKRDLARNSVLTIIERDNER
                     VSNNPMIQQTDNADSRVRSLEVPENE"
     gene            complement(5110..6084)
                     /locus_tag="ACHJ69_002962"
     CDS             complement(5110..6084)
                     /locus_tag="ACHJ69_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFO5015904.1"
                     /translation="MMSRSFRLRAGALLLSVAALPLASASLNTASIVASAAAPDCISW
                     RVSGICYWLYCSGFSCKVRTSVKVTHYIPEVVVSTYTAPGGNPWKEMALVSQTAGGVE
                     NSITKGLAGLSAGGGNPADMKAQGERRSAIRFKYADALGHPATCLIGGMVPGFSCDSP
                     VTPAMPYFLSTLDSLVWRTGMPESLYPEALIPGKREIGSQAEENLWGSVYPRSGFVMQ
                     TDDDKAAAVVAQRVADIISRTGEPHVYKSIQTRKKDGFWPPDPVEENTGTRNHKWQRL
                     TPSVSRSCAVFPDGDHQTAEDGNAAFALWQPYSCCKKRGQRFLGSTNF"
     gene            complement(6081..6479)
                     /locus_tag="ACHJ69_002963"
     CDS             complement(6081..6479)
                     /locus_tag="ACHJ69_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HFO5015905.1"
                     /translation="MHRIIFSSLFFLPTAVSADITLYTDSQHPPVNPPSDVRVILLDG
                     PEQLQARFFGALPADAGEAEERVRARMQSPEWGAMQAELAERYREVAHAWSLGVKKYP
                     AVVFDDRDVVYGTTDVAEAAQLRAQEGVQP"
     gene            complement(6706..8796)
                     /gene="iha"
                     /locus_tag="ACHJ69_002964"
     CDS             complement(6706..8796)
                     /gene="iha"
                     /locus_tag="ACHJ69_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO5015906.1"
                     /translation="MRITTLASVIIPCLGFSASSMAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSRQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWTSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNIGARLDWKATEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHSFTFGTWKSYLNWNETENKGRELVQSVLKRDKWELAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGASTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(9187..9573)
                     /locus_tag="ACHJ69_002965"
     CDS             complement(9187..9573)
                     /locus_tag="ACHJ69_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO5015907.1"
                     /translation="MMYLKYPEPELQTAVHPGGVVFFLPPQGKPGVTEITQPHLLRLR
                     SHLNRRLGRLRRVVCQPQRVGMNSSVAVTLEGDTGKVYLLVSVGGHECWPSEEEYTHL
                     RWYIQVVDAADLFYLLLWLNGMEAEK"
     gene            complement(9570..12386)
                     /locus_tag="ACHJ69_002966"
     CDS             complement(9570..12386)
                     /locus_tag="ACHJ69_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFO5015908.1"
                     /translation="MAFTLFKCRNVTTPSETETAGQGFVPPVPPGAQPLKRPGQMTQE
                     EEVRVYHAGPSVIDFLPWVEYLDEAQCLLLDDGMSVGAVYDVTPAATEGRTEERLEQI
                     RDMVEDALQDSFDEYDTHPWVVQFFCQDENDVDAYLDYLKGYVKPHAQGTAFTEAWLG
                     EMECHLRGIARPEGFFTDTLVTGQPWRGQQRRTRMVIYRRIAKNNSDPMPPVAMLNQV
                     CDRVVSALGSAGVRCHRMNGLQVHSWLLRLFNPAPSWISREDLYRMASRADPRENDAD
                     VMPVMTDFAESLWFNPPHSDPENGVWWIDDLPHCAVVVEKLRTPPEPGAITGEMSRGE
                     KTVNALMDTFPEGTVLCMTIVVQPQDTLEERFTRLSKNAVGENTESIRARQDVKVVKE
                     LLGNRHKLYRAGITFLLRAGDMDSLKRKRVELTTVLLGAGLQPVRPEFDVGPLNSWLR
                     ALPMCFDPDMDRKQWHTRLMWVQHLAGLLPVTGRETGTGHPGFSFFNRGGDVLTFDPL
                     NKLDRTQNAHLLLFGPTGAGKSATLCASLSQVMAVHRPRLFIAEAGNSFGLLADYFES
                     LGLSVNKISVKPGSGVTLPPFADAHKLVEEGQTLQSVDEHSLPDLDGDEEEEKRDILG
                     EMEISARMMITGGDPKEEASLKRADRAMIREALLMATHATYREGRQMLPEDLQVALRE
                     ISCDAGRNEVRRAKAAEMAESLGMFTQKGSFEAELFNREGELWPEADVTLIDLGHLAR
                     EGYEAQMALTMVSMTNMINNIAERDQYLGRDIVFAVDEAHIVTVNPLLSPYMTKVVKM
                     WRKLGAWLWLATQNLKDYPDISEKMLNMAEWWVCLTMPPEEVNNIARFKSLTEEQKAV
                     LMSASKLSGCYTEGVVLAKKIEALFRAVPPSLYLALGMTEKDEKAERRMLMKEHNCSE
                     LEAAFHVARKLDRLRGLSAVESAA"
     gene            complement(12386..12799)
                     /locus_tag="ACHJ69_002967"
     CDS             complement(12386..12799)
                     /locus_tag="ACHJ69_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFO5015909.1"
                     /translation="MKRLRLVMCIPLAALLLSGCSTSKEELLPAGDQTMLELWQGKTT
                     GGSQRHALAARETLWRPLTSDEKQQVHREGQSYSRTQESEISQQFPRLPNPDMVMYVF
                     PHLAGGTSPVPGYSTVFPFYSRTQYALPGERTEAM"
     gene            complement(12817..14295)
                     /locus_tag="ACHJ69_002968"
     CDS             complement(12817..14295)
                     /locus_tag="ACHJ69_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFO5015910.1"
                     /translation="MQIKSNALVKFVVPAVVLVGLAVTMKSCKTQENVADRGEQARAL
                     SGLSSEELRALGVEGDTPEDTLRTLVARMNQVRDNQRTLEKQNSQLLKDNEQLRRRST
                     DVSGQVNDAVAGGQKTYEQKQRELQEQQHSLMSQIDAMKDQLSRAETLSRRQESDIPP
                     GLGLDAGGGSGVVISQGADGLMWVSPADQSQDDPLSSSGSKGGPRFPTSFLEDNALSR
                     QKAEYEQQVKGYSNEQQRATTQAVYTLPENSTLTGSRAMTALLGRVPINGTVTDPYPF
                     KILVGRDNLTANGIELPDVEGAIVSGTASGDWTLSCVRGQVNSITFVFADGTVRTLPS
                     PDVNGTTNSNQQAKGNGTGGIGWISDEDGIPCISGTRKSNASSYLPTIFALSAAGAAG
                     DALSEGQYTSQNNVNGITSTLTGSAGQAVLGKALSGGMNETADWVRQRYGQTFDAIYV
                     PPGKALAVHITRQLAIDYEDQGRRVRYDFALPGEANDHGGLD"
     gene            complement(14285..15193)
                     /locus_tag="ACHJ69_002969"
     CDS             complement(14285..15193)
                     /locus_tag="ACHJ69_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFO5015911.1"
                     /translation="MKADIVCSLLRPAVMMMSLSVLSLTAQADELMKWERIPLNIPLK
                     VGQERVIFADKNVRVGFPPALNGKLRVQSTGGAVYLKADSAFPQTRVQLQDVESGEMI
                     LLDVTAGEKGTTEPVRIVYDGDVSTVSRAAGSDVQTVKSRSSNGKPGTDNSTQARRTE
                     PRYAEPLPVLLTRYAAQSLYAPLRTLEPVAGIRPVVPHLPRQITTVYPSEPLTVSPVA
                     AWAAGGRTVVALKLTSTVSRRIVLDPRALQGRFVAATFQHRWLGAAGTPEDTTMLYVV
                     TAGSPEKAFVEEPVTSRRGGEVTHAD"
     gene            complement(15190..15837)
                     /locus_tag="ACHJ69_002970"
     CDS             complement(15190..15837)
                     /locus_tag="ACHJ69_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFO5015912.1"
                     /translation="MSRFRHALKDRDQHILTLRVFSGVLCGLLLMVTIGWMTAPDRLT
                     IHNPPDLRSGSTRPWWEVPPSTVYSFAFYIFQQLNAWPKNGEVDYPAKIHALSAYLTP
                     SCREILETDARRRKDAGELLDRVRVVYEIPEHGYGGRYNRDPVTVEGRDNWLVRLDLV
                     ADEYFHAEPVKRALIRYPMKVMRMEGDPERNPFGLVLDCYSSTPQRLEAVKGGGE"
     gene            complement(15834..16199)
                     /locus_tag="ACHJ69_002971"
     CDS             complement(15834..16199)
                     /locus_tag="ACHJ69_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFO5015913.1"
                     /translation="METIRFLPDRLNAEPTVFRGFTTPELGLAALTGFFTGLLWSLPF
                     VPVFGWVIIPTGALLTPLVLVWTGGVWISRLKRGKPDNWLWQRLEEKKAALGVGRSGL
                     IREARGWSLRRTRVRGGKS"
     gene            complement(16219..16557)
                     /locus_tag="ACHJ69_002972"
     CDS             complement(16219..16557)
                     /locus_tag="ACHJ69_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO5015914.1"
                     /translation="MARLAGMAMVTFLLGSLTPALADLPQIEPPSSGGGGGLFGQIKG
                     YFQDGIVLIGLVIAAAAFVKVAEAAVTTFSEVRDGKTGWGQFGAIIVVGVVLLVAVIW
                     LLGKSASIIL"
     gene            complement(16627..16869)
                     /locus_tag="ACHJ69_002973"
     CDS             complement(16627..16869)
                     /locus_tag="ACHJ69_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFO5015915.1"
                     /translation="MALTSAQAAAFKVASGDVDLNVLHLSSLGLLIAVLFLWAAWGLS
                     DVWSGWRNGDVRESAFIWFTARTVLLLVASIWMFAG"
     gene            complement(16869..17153)
                     /locus_tag="ACHJ69_002974"
     CDS             complement(16869..17153)
                     /locus_tag="ACHJ69_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016190711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFO5015916.1"
                     /translation="MMLLVTLVLPVQAAEKDELATVMRQLDQVRAALDRARTASLQAT
                     PHDGRYFFDYPRAQADIQTMQEGIDRYLTPSRAQPRNGSAVTGEYRRERP"
     gene            complement(18160..18921)
                     /locus_tag="ACHJ69_002975"
     CDS             complement(18160..18921)
                     /locus_tag="ACHJ69_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO5015917.1"
                     /translation="MAEARRDAERSPQSRPPRQHGLLYNLVWGWPWALVGLILSSLLL
                     SLLIEYAGMTFFWPDEGAAHSRAVMNTELGWLSTEFTRSLLLSTPSVTVPGWISVAYQ
                     WLFVESGMQSWLQPQQAVTAVGRSVSQFSGELSQWLAYHLHDYLLATVYVTVVTMVRV
                     IILVLSVPLFVMVILVAVVEGLGRRDLRRYGAAYESSFVYHHAKKSIKPAIYIPCILY
                     LSWPTAVYPNLLIWPAAMLLGMAVTVTMASFKKYL"
     gene            complement(18902..21019)
                     /gene="traD"
                     /locus_tag="ACHJ69_002976"
     CDS             complement(18902..21019)
                     /gene="traD"
                     /locus_tag="ACHJ69_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO5015918.1"
                     /translation="MSNRYVIEALLRPAVEFNTAVVAATAAGVCVTAPWAVALAPSVS
                     YVTAAGFGVLAAVRFRQGMKIIRYRRNLRRLPRYVMSTRQIPVSRQRLFLGRGFRWTQ
                     KHTQRLQDTLRPEVAHYLLPGGFYRAARWLEMKTEHRLPGLGQWLRRDSPLNPVRPLP
                     PVGGNPALHGIEPDEQDVTLALGERVGHTIVYGTTRVGKTRLAELLVTQDIRRGEVTI
                     VFDPKGDADLLLRVWAEAHRAGRGDELYIFHLGWPEISARYNAVGRFGRVSEVASRVA
                     GQLSGEGNSAAFREFAWRFVNIIARALVALGERPDYMLIMRYVNNIADLYIRYAGKVI
                     RERLPGLEQIIANNQSVLSEEDVPRTMQNQPDAVRIWSIEMALSSEEGKKIYDPILDG
                     LRSAVRYDRTYFDKIVASLLPLLEKLTTGRIAELLAPDYLDMSDARPIFDWEQVIRKK
                     AVVYIGLDALSDSEVASAVGNSMFADLVSVAGHIYKHGMHAGLPAGGAGKNVVNLHCD
                     EFNELMGDEFIPLINKGGGAGMQVTAYTQTSSDIEARIGNAAKTAQVQGNFNNMIMLR
                     VRENRTAELLTTQLPQVEIYTKTLVSGHQDTADVSAGQDFTSSTQDRVGTVKVPLLEP
                     ADIVTLPKGQAFALLEGGQLWKIRMPLPAGDTDDVLMPESISRVAEAMRQNYHSGEGW
                     WNAPPSVQAIATEEGGDHGGSTS"
     gene            complement(21012..21800)
                     /locus_tag="ACHJ69_002977"
     CDS             complement(21012..21800)
                     /locus_tag="ACHJ69_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015919.1"
                     /translation="MNVSFTEQVQRNMPQWANEPLRAWDAAEFAAMSDFITEHYWTGQ
                     GSINVFRIVGTDHPQYAGMSWLELLERGKRMDINIPLVEKNPGYYTQAEQLHAGMSFV
                     STDGIHWYVSADGNHRSCLARFLFHLQGEERTQLHNVAQSIYHADRAFWSACREIHNL
                     ADVLSRHGVYLRLQTRRQSVSREDLACWKVDRFRTEAQLTVDDGRAGGHASAEYKALL
                     LNAADAWREVRRLQNRAEALSASPETDIPRSWWLRLLQRGRGHE"
     gene            complement(21814..22412)
                     /locus_tag="ACHJ69_002978"
                     /pseudo
     CDS             complement(21814..22412)
                     /locus_tag="ACHJ69_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946004.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(22412..22918)
                     /locus_tag="ACHJ69_002979"
     CDS             complement(22412..22918)
                     /locus_tag="ACHJ69_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO5015920.1"
                     /translation="MTAVWPLLLCAAQAGAELRVIADLGGSDASVFYEAINRQEGSST
                     PPPVTAPLPAQGETLLLPVTTQEMTPGEVTPRPLQLPGIGALFLVGDDALSRQWLQAN
                     AGALRERQAVGMVVSVADENSLRTLRELAPGVMMVPSSGSALARRLQLNHWPVLITDS
                     GISGVVTP"
     gene            complement(22939..23601)
                     /locus_tag="ACHJ69_002980"
     CDS             complement(22939..23601)
                     /locus_tag="ACHJ69_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase"
                     /protein_id="HFO5015921.1"
                     /translation="MARGSLLTMALLSMAVSSLCRAGAHDIPEGYVRVAAVHGVPAEV
                     LYAVSLTETVMAPSAIAGVMKKRDADFRLPSVARPWPWTINVAGKGYRYASRLEAWQA
                     LQVFLTRYPARRIDVGIAQVNLGWNGHRFSSTWAAFDPYISLNTAATILRECRRRHPV
                     SWLQAAGCYHYPAGGAPAVRYTAVVRQHLERLHRDAVKTAEQHDGLTAMHPRYIWTEP
                     RE"
     gene            complement(23580..24302)
                     /locus_tag="ACHJ69_002981"
     CDS             complement(23580..24302)
                     /locus_tag="ACHJ69_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFO5015922.1"
                     /translation="MKMKNLSPVLMLLISLQTVAAVRESAPEGTRTQASSQVSTTTQA
                     LQQQAAQWGVTEEDYRRYRALMEGPRGMQSPGLDPLTVLGIEARTDAERRQLAEKWVK
                     AEYERTEKELAFQREVNAAWKRLYPGVMPVSLGKGGGLLNDNGGRLALFVKAKDCGTC
                     DARLASVLSSGRQVDIYLVDSQGSDNLLRQWALEHDIPVDRVRSRHITLNHDAGRWLR
                     FGEGRMPVVLQQGADGWRVAAF"
     gene            complement(24307..25074)
                     /locus_tag="ACHJ69_002982"
     CDS             complement(24307..25074)
                     /locus_tag="ACHJ69_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015923.1"
                     /translation="MSEKKQKTLPSVHSSPLDGWTCVSPMLSVGFVLTVLVATILAVC
                     GLYRYSGEQDERIAALEGQMKTYSDALALSGSELEPLRSRLAETKKQLKSVTERLTLL
                     EQRPVLTEEEHARTGQMAASLASLQATLAQQGETLRAYDLRLEKLEKAPFTPVSPSSA
                     KGTGTVKSTPAVTPRQKKGSSGISVISRSAPFVLTGTEQRGGRTYAAVAPRGFTRLSD
                     VTLLGEGESTAGWMLERAGRGEASFRVNGRQVRVMVE"
     gene            complement(25071..25649)
                     /locus_tag="ACHJ69_002983"
     CDS             complement(25071..25649)
                     /locus_tag="ACHJ69_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HFO5015924.1"
                     /translation="MKTSLLLSVLSACVLLTGCQGQQKLLQRDAAVPVPSVTVSNNVR
                     PASPDMYAGTTVPEVVRYDRYLLVNTHPDAVQRDPLSQVIDIRIPASLKPTVADAMRY
                     ALKQSGYTLCATGPANGVLYRQALPAVQYQTGPLRLRTALQMMAGPAWQLEVDDVQRV
                     VCHSLREGYQLPPGQLAPVPAGVRGAGAELKK"
BASE COUNT         6033 a         8114 c         6090 g         5582 t
ORIGIN      
        1 aacagacgtc tgtgtctgat tttatttatt atttaatgga aaaacacaac aaatatcccg
       61 tgtatgtatt acatatacca tcaaacaata caatagaaat aggctataat ataatgaatg
      121 aaaatcttgt cattggaaaa ggaatacatt tcaggacact aagcaatcgt tctttatatt
      181 tcaccctgac acaaccccca ttaatcgcca caataaaaga tgatgtattt tgcactcttc
      241 atgattacta taaccacaat aatgaaacca aatccaccat tgacaattac atatcaaaaa
      301 tacaggataa tcacaatacg ccatggatat taaataacga aagcgttcag aaataaaaaa
      361 cacagagaca acgatcagga taacacaaaa cagcataatc acatgaaccg gaaattctat
      421 ttcaaaaata tcctgtgggg aatgtgcccc attataacat ccatattaca tcttatatat
      481 aacacaccaa cagaaacgtc gataaaagtt gacttatttc tgatactaac tggcctggcc
      541 cttccttgtt caatatacct gacaaaaaca atcacactaa aatacacatc catatcattc
      601 tggaaaaaac cttttttctg gtcttatggt atatatcatt ttcatcttgt ttatataatg
      661 atatgtatgg ttttagcaat accttctgca attattgttt tactctttca cttttatcaa
      721 aacatcacca gaaacaggaa atgacagtcg caataatgaa cataaaaaga caaaccagga
      781 tggcagtaaa tcaataaagt accatttcat atgaatcatc agaaaaacgg tggacgcgat
      841 atccggcacg gggcaaaaac acagcaacaa aaaataaacc ggatatcgcg cccggtactt
      901 ttctaactca acttcaggca ttgtgcaagt tttgatcgtc ggtggattca gagccatgcc
      961 ccattgattc aggctgaaga ctcacatatc attgaagaga ctagaatctc attgctgtga
     1021 aattccttac agaatcttca gccccatttt atggggcttt ttttggctaa acccacgctg
     1081 tcaccaggcc ttcagtatgc gaataaaaaa gcccgcagtt tttacactgc aggcttcagg
     1141 ttttctttgt gatttgcatg caagggtgcg actttcgacc gaaccttctg caaccggata
     1201 gcgggggctt tcacccccgc ttcggtttct tacggtttac actacaagaa cgccgcaaat
     1261 tctgaccaca tgatgttaag tctgaattca cacggaagtc tgaattcaca cggtgacgcg
     1321 tgtaacatcc agacggtcaa caacatggtt aagcataaaa tgctcacgat gatcgttttt
     1381 tgcggcatat attgaacatt tatcatacag cacgtatgat atcactgtga taactacctg
     1441 ctctggggag ctgcgctatg aaaaacgaag cagaaaaagc tatgtggagt tgtaaaacct
     1501 ctgacacacc atggactttc agccgcctgc tgaaaaaggc tatctgtgcg gttttaccgg
     1561 attctggtcc acacacggtt catcgtatca ataagaaaga aaacaattat ttaacatctg
     1621 ttttatctga tatatgcgat tcaaatgaaa aagattcgaa tgaagcgatg ttcaatccct
     1681 ttcatcctct ttatcgagat catcatccta ttgatagtag agaatggagt ggtagccctt
     1741 acaaaacgcc agaataaaaa tatggcgata ttcatgacaa atatcgccat aaaaaacagg
     1801 tagttatttt attcttttaa caaaacttcc agcatgctgg cctgcttgtt gtgtaacatg
     1861 ccctgctcga ttgaatccct cagaaatagc ctgtccgata ttaactccag cccaggataa
     1921 tgcccccagc cacaccgccg gtaatacgag aaacatcgtg cccatcacca gattcatgat
     1981 gatatcgtcc gacgtgttct gcagccccat catattcaga ctcgaatgtg tatctgaacc
     2041 atacaacgcc gttatcatcc agctgtccag ccaccgcgcc agttcccacc agaacgtcag
     2101 aaagttcagc gcaaacacga caaatgtcag cgtgatcagc gtactgaact catacgccgc
     2161 aaatgccagt accaacggga tcatgatata caccgccatc aacagaatac tctgcaccat
     2221 cggcagtgcc tgccgcatgg catcaaacgc cggaaacgcc cccagactgg cgacaccgga
     2281 acctgccgtt gcgccaatgc gtgaaatggc attcatcgtg gtcatgtctg cattaccacc
     2341 atatccccta tacacttctc ccccctgaga aatcgtcagg ttagccggac tgaccagccg
     2401 tttaataatc tcctcctgat aagtcgcctg gctgttcccc agaagcttca tcgctgccga
     2461 aagacgcagc cacagccctt catcaacttc ctccgtgacc cgctttttca gccccacact
     2521 gctgtcgttc caccactccc ggcacgttgg gtagcctccg cgccccgtat ccggacgacc
     2581 gctgtcacgg gcgtcattcc acggaaacgc cgcccttggc attctggact gcagcgtgcc
     2641 gtaatccccc tgcatgaacg tgctgctccc aatccagctg atatccgaca gcacctttct
     2701 gtccttcgtc ataccctggt cacgctgttt ccactggtac atcgccagcg agtagcagtc
     2761 attggtgaaa tcctgcaact catcggccag cgccctgttc gcaatgaacg tcctctgcac
     2821 ttcaaaacgc agttgccgca gatcaggcct gcagggaatg gacgccacgg ccgcctgtgt
     2881 cacccctttg gaaagcttgt gcacaacaac ccaccagatc ggggccgccg ccgtcttatc
     2941 tcccaggctg ctgataatcc cctgataacc ggtgtccccg ggggctttcg gcgtccacac
     3001 accacaggtc tgggcccggc tgcgatcaaa cttcagggta tccagcgtca cctgaaccag
     3061 cggtacacag gaagccacca tcacaaaaaa cgcaccatac aatgcattct cgatgcgggg
     3121 taacgagagc attcccttgt tgccttcgtc cgccccctct tccctcacct tcagccagat
     3181 accgatcact ttgaaagcca gaggaagcgc aaacagtccc gtgccaatca gcaaatccca
     3241 cagaccgtta ttgaccagcc agcccagcag ggtcagaaaa tattccagat agttattggt
     3301 cgtcactgct cagtcccccg cggcggaaaa aatcgcgtat tcgcacagca ggaaaaacag
     3361 cgcactgacc atacaaatcc gagcaaaacg cagacggtac tctgcccgga atcccggtgc
     3421 acgccagatt ttccggcatc cccatacaag cccgcagtaa atacaaaacc gccagcccag
     3481 ccatccgaaa cgggtttcgt ggaaccagag cctcagcgca tccacctcct gcgcatgacg
     3541 catacctgta tccgcaatca ggagcgctga cgccatgatg acaatcactg tcagccccca
     3601 tttccccagc agtttcatcc ctttcacaag cagcccggcc cggctcccgg gcaccatcag
     3661 cacggtttta ttcattttca gggacctcca gtgaacggac acggctgtca gcgttgtcgg
     3721 tctgctgaat catcgggtta ttactcacgc gttcattgtc acgctcaatg atggtcagca
     3781 cactgttacg cgccagatcc cgtttcagct ccatctcact tttcagggcg atgatctccc
     3841 tgtccagaga ggcaatacga cgatcgcctt cttccagcgc agcaggctgt gcagccgcat
     3901 acggttcaga cattcctgtg agcagcattc tgcgcatcac cagtgccgtt tccaccgtat
     3961 cagccatcgc cagttcaccg gccagacggg aggtcaggga catattgtcc ggatcgcgac
     4021 gcagcgcatg tatcaccccg gcagataccg ccagactgcc ggtcttcagt tttgccagat
     4081 tctctgccgt gggtttatcc tgcccgttca ccaggcgaac cagctgctcc gtatttatgc
     4141 gtgtcgcctc ctcaagcatc ggcgcaaacc ctgttccggc caccgccgtt cccggctgct
     4201 ggtcggagtc accactggta cattccttcg catccgtaca ggtgcgcatt gaacgatcac
     4261 ccagtacctt caccacagcg ctggccgcct cctctgcatt cctgaatttc tcacaggcac
     4321 tgccattaca gcttcccgca ccaacaccgg atgtactggt caccggcaga ctgttcatca
     4381 tgttaaaccc cgcggccgtc atgtcatgaa ccggacggat ggcgggctga ccggcaccac
     4441 cacgttgctg accaccaatc catttctgcc cctctttccc cgttgcctgg cgtccggcgt
     4501 catcaccttt aacggcatca ccgtcactgg tattaacaac ctttttgtac tcctccatca
     4561 tcgcggcctg cgtccacttg ctgcgatcgg aaaaatccat catccgtttc gccatgttct
     4621 ggcagttcag ttgtgccttg tcgaacgcca cgtttgcctg taacacgcca ttcgtgatca
     4681 tgtcgtagag gccgggattc gcacgctgta tcaccattgc aggaaggctg gagaccgcac
     4741 cggtcgcccc ctgaatcaca tcccccatca ggtttttaaa gccgtcagtg atcccgttga
     4801 gctggtttcc tactgtggtt ttcagatcga agttcccgca catcagatcg ctgctccagc
     4861 ccaggtccag cccgagcttc tgcatgttac tgcgcgtcgc cggctgggaa ataacggagc
     4921 caccgccaag ggtgtaaaac agcttgtccg acaccgcacc atccaccgat tttccgtaac
     4981 caaacgtgct gtcattcagt tttagcagcg agagaatgct gtctgccatg accggcgtac
     5041 tgcaggacag catcaccatc atcaccatca tcaccagagt ctttaccttc atcgtcatca
     5101 tccgtctcct taaaaattcg tactgccaag gaaccgctgc ccccgcttct tgcagcaact
     5161 gtagggctgc cagagcgcaa aagcggcatt accatcctcc gcggtctgat ggtctccgtc
     5221 aggaaacacg gcacaggagc gggagacgga gggcgtcagt ctctgccact tgtggttacg
     5281 ggtgcccgta ttttcctcaa ccggatccgg cggccagaaa ccatcctttt tccgggtctg
     5341 tattgattta taaacatgcg gctcaccggt ccggctgatg atgtcagcca cccgctgggc
     5401 aaccacggcc gctgccttgt catcatcggt ctgcatcaca aaacctgaac ggggataaac
     5461 actgccccac aggttttctt cagcctgact gccgatttca cgtttaccgg gaatgagggc
     5521 ttccggatac agggattcag gcattccggt ccgccagacg agactgtcca gggtactcag
     5581 gaaataaggc attgccggtg tcaccggcga atcgcagctg aatcccggca ccattccgcc
     5641 aataagacac gttgccggat gccccagcgc gtcggcatat ttaaaacgaa tggccgaacg
     5701 acgctctccc tgagctttca tatccgcagg atttccccca ccggcactca accccgccag
     5761 gcctttcgta atagaattct ccacccctcc ggcagtctga ctgaccagcg ccatctcttt
     5821 ccagggatta ccacccggtg cggtgtaggt ggataccacc acttccggaa tataatgtgt
     5881 cactttcacc gatgttctga ccttacagct gaacccggag cagtacagcc agtagcaaat
     5941 tccgctgacc cgccagctga tacaatccgg tgctgccgca ctggccacaa tactggctgt
     6001 attcagtgag gctgacgcca gaggcagagc tgcaacactc agcagcaggg ccccggcacg
     6061 cagacgaaat gaccggctca tcatggctga actccttcct gtgctctcag ttgtgcggcc
     6121 tctgccacat ccgtggtacc gtaaaccacg tcacgatcat caaagaccac ggccggatat
     6181 ttcttcacgc ccagtgacca tgcatgcgcc acctcccggt aacgctcagc cagctccgcc
     6241 tgcatcgccc cccactccgg ggactgcata cgcgctctga ctctttcttc ggcctcaccg
     6301 gcatcggcgg gcaatgcacc aaaaaaacga gcctgcagtt gttcaggacc atccagaagg
     6361 atcacccgca cgtctgaagg cgggtttaca ggaggatgtt gagaatcggt ataaagggtt
     6421 atgtcggctg acaccgccgt cggcaggaaa aacagggaac tgaatataat ccggtgcatg
     6481 gtacgcctcc gtcagacaat atctgaatga cagcgtgcgc agataccaga ctaatagcag
     6541 caataaatta aaaacgcgag gataaaaatt aatatgaaga tgcatggtgc acaaaacggt
     6601 atctgaacag aaatcagaca ccgggaatca tataacgtag ccactgaaca tccaggagct
     6661 acagtggcaa cgtatgctac cgtcagtgat acggttttgt gattatcaga actgatagtt
     6721 cagtgacatc cagtaatttc gctcaggtat cacatagccc gttgtcgatg ctcccgtctg
     6781 gaagtaatca ccggcataaa gcgtactctt accagcactg tacaggctca cgtcactgta
     6841 atccttgttg agcaggttat tcaccgcagc attcagcgtc agggcatccg tcatcttcca
     6901 cgacagacct gcatccacca ccgtccaggc tttcaggtat tctcctttct catcatacac
     6961 tttcttctgt acagcactca gcgacgaata attctgggtg aaacgtggtg ttttcccgcg
     7021 ataacgggca cccagccatg atgccacctc ttcggtgatc tgccagttca gtttcgcatt
     7081 caccatgtgt tcaggggtat aactcagcgg cgcacctttg ttatcaccat cacgttgttc
     7141 acttcgggtc caggtgtaat tcagtgacag cgtgacatcc tctgaccaca gcggcaatgt
     7201 gccggcaaat tccacaccgt gcaaccgggc ctttccgctg tttacatagc tattggtgtt
     7261 atcatttatg gaataagaga caatcttgtt ggagaagtca gtcataaaac ctgtgacatt
     7321 ggcattcaga ccggcggggt tatcgtaata caccccagcc tcataactga cgctctcttc
     7381 cggcttcagg tcggggttac caagtagatt tgtttttccc tgcccggaca caccactaat
     7441 ccctttatgt agctgcccca ttctgggtgc cttatatccc gtggtcacac cgcctttcag
     7501 cgtccaggca tctgccacat cccagaccag atatgcacgc ggactgaagt gtcccccgaa
     7561 ttgctcatga tgttcatagc ggctgcccgc agtcagcgca agtgcatccg tgagatgcca
     7621 ttcatcctca gcaaataccg accagctttt ctgccggaaa gtttcacctg tgctggcaag
     7681 gacaactccg tctttcatgg acgagctctg aaactcgccc cccaccgtaa ccagatgaga
     7741 ttctcccaga ggggtaagca gtaatgaatt caggataagg ttagattcct taagctcccg
     7801 cggctgaccg gcaagctccc atttgtcgcg cttcagtaca ctttgtacaa gttcacgacc
     7861 tttattttcc gtctcgttcc agttcagata cgatttccag gtaccgaagg tgaaagaatg
     7921 atcatagcca gctgaaattt tgtttcgctc atagcgcaag gtacggtcat atcccccggt
     7981 cagactcccc agttgcccgt cccggttatc ataacgctgc cgggtggtat ccatatcaaa
     8041 ccagagtaca tcctgctccg tagctttcca gtcaagacgc gcaccaatat tataattctg
     8101 tgactccgtg ggataaggaa tacgcgtggc tgctgtgtcg ctcagtgatg tgaccgatga
     8161 tccctgacgc tgctgtgtgc tgccccggac ctgcagactg acagaatcat ccacaagggg
     8221 accactggtc cagaaattaa actggctgct gttaccccat ttgttgcttt cctgcagatt
     8281 cagccccgca ttgacggaag agagccattt gtctgcattc tttctggtaa tgatattcac
     8341 cacaccgccc atcgcatcag agccatacag tgtggacatc ggccccctga taacctcaat
     8401 acgctcaatg gcggccagag gaggcatgaa cccggtattc atggcagaaa aaccgttggg
     8461 agtcacgtca ctgctcccgc cctgacgaac accatcaatc agtatcagcg tgtaactggc
     8521 aggcattcca cggatgctga tttccagccc accggtttta cccgtaccac tttcaacatc
     8581 cacaccctct acagatctca gagcctccgc cagatcgtgg tactgcctgg actgcaattc
     8641 ctcctggctg atcacagaga cactggcggc tgcgttagtc agttttttct catagccgga
     8701 tgccgagaca atcatcacat cctctgcagc agccatgctg ctggctgaga atccgaggca
     8761 gggaatgatg acagaagcca gagtggttat tcgcatatta ctaatctccg atcatattaa
     8821 ccggcaagac aatacacaaa tacaaacaat aatgagaata atttatattt actttttaca
     8881 aaaactgaag attcataata aaaaaaaaga tatacaacga ctgcagcaac acgcctgtta
     8941 acggctatct gtctgacaga ttcgattctg aatgtaaaac cggttactgc agaaacgtat
     9001 gctcacagcc agtcggccat aaatgctctc tggaggggca cgagtgaaat gattcagtca
     9061 atgagttctt ccggtgaata cggataaaaa tattgccgta atgtaataaa cgtgttaatt
     9121 aaaaaacgcg tgtaccggca ggtatgaatt gatcccgtaa cccatctgcc ggttacacgc
     9181 tggcacttat ttttctgcct ccataccatt cagccacaac agtagataga acaaatcggc
     9241 cgcatccaca acctggatat accatctcag atgcgtatac tcctcctctg acggccagca
     9301 ttcatgacca cccacggaaa caagcagata gacctttccg gtatcacctt ccagcgtcac
     9361 cgccacgctg ctgttcatgc caacccgctg gggctggcat acaacacgac gcagcctgcc
     9421 cagacgacga ttaaggtgtg agcgcagccg cagaagatgc ggctgtgtta tctcggtcac
     9481 cccgggctta ccctgtggcg gcaaaaaaaa cacaacgcca cccggatgta ccgctgtctg
     9541 cagctccggc tccggatatt tcagatacat catgccgcac tctccacggc ggaaagtccc
     9601 cgcagtctgt ccagtttacg ggccacatgg aacgcagcct ccagttcgct gcagttatgt
     9661 tccttcatca gcatgcgccg ctcagccttc tcgtccttct ccgtcatccc cagcgccaga
     9721 taaagactcg gtggcaccgc acggaacagc gcctcaatct tcttcgccag gacaaccccc
     9781 tccgtataac agccggacag tttgctcgcc gacatcagca ccgccttctg ctcctccgtc
     9841 agggatttaa aacgggcaat gttgttcacc tcttccggcg gcatcgtcag acacacccac
     9901 cattcagcca tgttcagcat tttctctgat atatccggat agtccttcag gttctgcgtc
     9961 gcaagccaca gccaggctcc cagcttacgc cacattttca ccaccttcgt catatagggc
    10021 gagagcagcg gattcaccgt cacgatatgc gcctcatcca cggcaaacac aatatcacgc
    10081 cccagatact ggtcacgttc agcgatgttg ttaatcatat tggtcattga caccatcgtc
    10141 agcgccatct gcgcctcata cccctcacgg gccaggtgcc ccaggtctat cagcgtcaca
    10201 tccgcttccg gccacagttc gccttcacgg ttgaacagct ccgcttcaaa actccctttc
    10261 tgggtgaaca ttcccagtga ttccgccatc tctgccgcct ttgcccggcg tacctcgtta
    10321 cgtcccgcat cacaggagat ttcccggagt gccacctgca aatcttccgg cagcatctgc
    10381 cgcccttccc ggtacgtcgc gtgtgtggcc atcagcagcg cttcacggat catcgcccgg
    10441 tccgcacgtt tgagtgaggc ctcttccttc ggatcaccgc cggtgatcat catcctggca
    10501 gagatttcca tttctcccag aatgtcgcgt ttctcctcct cttcatcgcc atccagatcg
    10561 ggcagcgagt gctcatccac actctgcagg gtctgccctt cttcaaccag cttatgcgca
    10621 tccgcaaagg gcggcagtgt cacaccgctc cccggtttga cactgatttt gttcactgaa
    10681 agcccgagac tttcaaagta atcggccagc agaccaaacg agttacccgc ctcggcaata
    10741 aacaaccggg gacgatgcac ggccatcacc tgtgacagag aggcacacag cgtggcagac
    10801 tttcctgccc ccgtgggccc gaacagcaac agatgggcat tctgggtacg atccagtttg
    10861 ttcaggggat caaacgtcag gacatctccc ccgcggttaa aaaaactgaa gccgggatgc
    10921 cctgtacctg tttcccggcc ggtcaccggc agaagccctg cgagatgctg tacccacatc
    10981 agacgtgtgt gccactgctt cctgtccata tccggatcaa agcacattgg cagcgcacgc
    11041 agccagctgt tcagcggccc cacatcaaac tccggtcgca ccggctgcag gcctgcaccc
    11101 agcagaaccg tggtcagctc cacccgttta cgtttcaggc tgtccatatc accggcccgc
    11161 agaaggaagg tgatccccgc acgatagagt ttatgccggt tacccagcaa ctccttcacc
    11221 accttcacat cctgacgtgc ccggatggac tccgtattct cacccacggc atttttcgac
    11281 agccgggtaa accgctcctc cagggtgtcc tgtggctgta ccacaatggt catgcacagc
    11341 accgttcctt ccggaaacgt atccatcagc gcattcaccg ttttttctcc ccgggacatc
    11401 tcaccggtaa tggctccggg ctccggtggc gtacggagct tctccaccac cacggcacaa
    11461 tgtggcagat catcaatcca ccacaccccg ttctccggat cggaatgggg cggattaaac
    11521 cacaggcttt ctgcaaagtc cgtcatcacc ggcataacat ccgcatcgtt ctcacgggga
    11581 tcagctcttg aggccatccg gtacaaatcc tcccggctaa tccaggacgg tgccggatta
    11641 aataaccgga gtaaccagct gtgaacctgc agaccgttca tccggtgaca gcgcaccccg
    11701 gcgctcccca gtgccgacac aacgcgatcg caaacctgat tcagcatggc caccggcggc
    11761 atcggatcac tgttgttctt cgcgatacga cgataaatca ccatacgggt tcgccgctgc
    11821 tgtccccgcc acggctgacc ggtaaccagc gtgtctgtaa aaaagccttc cgggcgggca
    11881 attccccgca aatggcattc catttcgccc agccaggctt ccgtaaacgc cgttccctgc
    11941 gcgtgaggtt tcacataccc tttcagataa tcaagatacg catccacgtc attttcatcc
    12001 tggcagaaga actgcaccac ccagggatgc gtgtcatact catcaaagct gtcctgcagt
    12061 gcgtcctcca ccatgtccct gatctgttca agacgttcct ccgtacgccc ttcggtcgcc
    12121 gccggcgtca cgtcataaac agcccctacc gacattccgt catccagcag cagacactgt
    12181 gcctcatcca ggtactccac ccagggaaga aaatcgataa ctgacggccc ggcatgataa
    12241 acccggacct cctcttcctg cgtcatctgc ccgggacgtt tcaggggctg tgccccgggc
    12301 gggacaggcg gtacaaaacc ctgtcctgcc gtctccgttt ctgatggcgt ggtgacattg
    12361 cgacatttaa acagtgtgaa tgccattaca tcgcctccgt acgctcaccg ggtaacgcat
    12421 actgcgttct gctgtaaaaa ggaaataccg tgctgtatcc cggcaccggt gacgtcccgc
    12481 cggcaagatg cgggaatacg tacatcacca tgtcagggtt tggcagacgg ggaaactgct
    12541 ggctgatttc actttcctgc gtcctgctgt agctctgccc ctccctgtgt acctgctgtt
    12601 tctcatctga cgtcaggggc cgccacagtg tctccctggc ggccagcgca tgtcgctggc
    12661 tcccccccgt tgttttcccc tgccagagtt caagcatggt ctgatcgcct gccggcagaa
    12721 gctcctcttt cgaggtggag cacccggata acagcagggc agccaggggg atacacatca
    12781 ctaaccgtaa tcgtttcaca aacgtattct ccctgttcag tcaagccccc cgtggtcgtt
    12841 tgcttcaccc ggcagggcga agtcataacg cacacggcgg ccttgatctt cataatcaat
    12901 agccagttgt cgggtaatat gtaccgccag cgctttcccc gggggaacat aaatggcatc
    12961 aaacgtctgc ccgtaacgct gcctcaccca gtctgccgtc tcattcattc caccggacag
    13021 cgccttacca agtacagcct gccccgcgct gccggtcagc gtggaggtga taccgttgac
    13081 attgttctgc gaggtatact gcccctcact cagggcatcc ccggctgcac cggcggctga
    13141 cagtgcaaat atcgttggca gataactcga cgcattggat ttacgggtac cgctgatgca
    13201 gggaataccg tcttcatcgg agatccagcc aatcccgcca gtaccattcc ccttcgcctg
    13261 ctgattgctg ttggtggtgc cgttgacatc cggtgacggc agcgtgcgca ccgtgccatc
    13321 agcaaaaaca aaggtgatac tgttcacctg cccgcgcaca caggaaagcg tccagtcacc
    13381 actggccgtg ccggagacaa tcgccccttc cacatccggc agctcaatgc cgtttgccgt
    13441 gaggttgtca cgcccgacca ggatcttgaa cgggtaagga tccgtcacag tgccgttgat
    13501 cggcacccgc cccagcaggg ccgtcatcgc acgactgcct gtcagcgttg aattttccgg
    13561 cagcgtgtaa accgcctgtg tggtggctcg ctgctgttca ttgctgtatc ccttcacctg
    13621 ctgctcatac tcagccttct gacgactcag tgcgttgtcc tcaaggaatg aggtcggaaa
    13681 acgagggcct ccctttgaac cactgctgct caggggatcg tcctgagact gatcggccgg
    13741 acttacccac atcagcccgt cagccccctg cgaaatcacc acgccactgc cgccgcccgc
    13801 atcaagcccg agccccggcg ggatatcgct ttcctggcgg cgactgagcg tctctgcccg
    13861 gctcagttga tctttcatgg cgtcaatctg cgacatcagg gaatgctgct gctcctgcag
    13921 ttcccgctgc ttctgctcgt aggtcttctg cccccctgcc acagcgtcat tcacctgacc
    13981 ggacacatca gtgctccgcc tgcgcagttg ctcgttatcc ttaagaagtt gcgagttctg
    14041 tttctccagc gttctctgat tgtcccgcac ctggttcata cgggcgacca gcgtccgtaa
    14101 tgtgtcttcc ggcgtgtcac cttccacccc aagagcccgt agctcttctg acgataatcc
    14161 ggataaggcc cgggcctgct ccccgcggtc agcaacattt tcctgcgttt tgcagctttt
    14221 catcgtgact gcaagcccca ccagaacaac agcgggcacc acaaatttca ccagcgcgtt
    14281 ggacttaatc tgcatgcgtc acctccccgc ccctgcggga cgtcacaggc tcttccacaa
    14341 acgccttctc cggactgccc gccgtcacca catacagcat cgtggtatct tccggcgtac
    14401 cggcagcacc cagccagcga tgctggaaag tcgccgccac aaaacgcccc tgaagggccc
    14461 ggggatcaag cacaatacgc ctgcttaccg tactggtgag tttcagggcc accaccgtcc
    14521 ggccaccggc tgcccatgcc gccaccggtg aaacagtcag cggctctgac ggataaaccg
    14581 tggtgatctg ccggggaaga tgtggcacca ccggtctgat ccccgccacc ggttcaaggg
    14641 tgcgcagtgg tgcatacagg gactgtgccg cataccgggt caacagcacc ggcaggggct
    14701 cagcataacg gggctccgtg cgacgggcct gagtactatt atccgtcccc ggcttcccgt
    14761 tgctgctgcg acttttcaca gtctgtacat cactcccggc agcacggctg accgtactca
    14821 cgtctccgtc atacacgata cgcacgggct ccgtggttcc cttttccccc gcagtgacat
    14881 ccagcaggat catttccccg ctttccacat cctgcagctg aacccgggtc tgcggaaatg
    14941 cactgtctgc cttcaggtag accgcaccac cggtactctg gacccgcagt tttccattca
    15001 gcgccggagg gaagcccacc cgcacattct tatccgcaaa aatcacccgc tcctgcccca
    15061 ccttcagcgg gatattcagc ggtatacgct cccacttcat cagttcatcc gcctgtgccg
    15121 tcagactcag cactgacaac gacatcatca tgacggcagg acgcagcaga ctgcacacaa
    15181 tgtcggcttt cattcaccac ctcccttaac ggcttccaga cgctgtggcg tggagctgta
    15241 gcagtccagc acaagaccaa acggattacg ctccggatcg ccctccatcc gcatcacttt
    15301 catcggatag cggatcagtg cccgtttgac tggctctgca tgaaaatact catccgccac
    15361 caggtcgaga cggacaagcc agttgtcccg cccctccacc gtgacagggt cacggttgta
    15421 acgcccgccg taaccgtgct cagggatctc atacaccaca cgaacacgat ccagcagttc
    15481 tcctgcatcc ttacgacgac gggcatccgt ctccaggatc tcccggcatg acggtgtcag
    15541 ataggccgac agtgcatgta ttttcgccgg ataatccacc tcgccatttt tcggccaggc
    15601 attcagttgc tgaaaaatgt agaacgcaaa ggaatagacc gttgagggag gaacttccca
    15661 ccacggacgc gtactgccgg aacgcagatc cggcggatta tgaatggtca gccggtcagg
    15721 ggccgtcatc cacccgatag tgaccataag aagaagtccg cacagcacac cgctgaacac
    15781 cctcagcgtc agaatatgct gatcacgatc cttcagggca tgacgaaaac ggctcatgat
    15841 ttaccccctc tgacacgggt acggcgcagg gaccagcccc gggcctccct gataagcccg
    15901 gagcgtccca cgcccagcgc cgcttttttc tcctcaagac gctgccatag ccagttatcc
    15961 ggtttccccc gcttcagacg gctgatccat acccccccgg tccagacaag aaccagcggg
    16021 gtcagcaggg cgccggtggg tataatcacc cacccgaaca ccggcacaaa aggaagtgac
    16081 cacagcagac cggtgaagaa cccggtcagt gcagccagcc ccagttccgg ggtggtgaac
    16141 cccctgaaca ccgtcggttc ggcgttcaga cggtcgggca gaaaacgaat ggtctccata
    16201 accagctccc actgttgctt acaggatgat ggacgcggat ttaccaagca gccagatgac
    16261 cgcgaccagc agtaccaccc ccactacaat aatggccccg aactgccccc agcccgtctt
    16321 gccgtcccgc acctctgaaa acgtggtaac ggccgcctcc gccactttca caaaggcagc
    16381 cgcggcgatc accagaccaa tcagcacaat gccatcctgg aaataacctt tgatttgccc
    16441 gaaaagtccg ccaccaccac cggacgacgg cggttcaatc tgcggcagat cggccagtgc
    16501 cggtgtcaga ctgcccagca gaaacgtcac catggccatt cccgccagac gcgccatcag
    16561 gcgcagacgg ctgacagtaa gccctttttt acgcatagat ttctccttaa aaagtcacac
    16621 catgaatcaa cccgcaaaca tccagatgct ggcaaccagc agcagaacag tgcgggcagt
    16681 aaaccagata aacgcagact ccctgacgtc accgtttcgc cagccagacc agacatccga
    16741 cagtccccag gcagcccaga gaaagagaac ggcaatcagt aatcccagcg aggacagatg
    16801 cagcacgttc agatccacat ctccggatgc caccttaaag gccgcagcct gtgccgacgt
    16861 cagtgccatc agggacgctc ccgccggtat tcaccggtca cggcactgcc gttacgtggt
    16921 tgcgcccggg agggcgtgag ataacggtcg atcccctcct gcatggtctg aatgtcagcc
    16981 tgagcacgcg gatagtcgaa aaaataacgc ccgtcgtggg gcgtggcctg aagggacgca
    17041 gttctggccc gatcaagcgc cgcacggacc tgatcaagct ggcgcatgac cgtcgccagc
    17101 tcatcctttt cagcggcctg aacgggcaga accagggtca cgagaagcat catcccggcg
    17161 gtccccgtca gaccggccgt tttccgggat aatgcaggaa gagaaaacat acgcacctcc
    17221 gtcagaatca atgggtgcgt acagcatgcg taaggcaggg ggagggatca gcgataaact
    17281 ctatttgcaa aactgaaaat ttttacggga gaatacacgg gtgtggataa aggcaaacaa
    17341 cccggagctc ctggcatgaa cgatatcatc agtataatta ttcttctggc agtctatctc
    17401 atcggcctga tattcattgt cctgattttc aatgacatag acataacatc gaaaaagaga
    17461 aagtacgaaa aaaactgaaa gaagcgataa taaatggcag tttacaaaat aatgatatct
    17521 atttactggc cgaacgctgg tctgtcagtc atgaacatat caaaagcgca ctgctgtcag
    17581 ttttcaatga ttttctgact gataacaact tttcacatca cgtcccccgg gtcagagaac
    17641 tgattacatg gtacgagaaa tacgccccat tcacagatct ccctgaggat ataaaacaac
    17701 agttacaaaa tataatcaat ttacccgatg ccagtgagtc agaagtgcag ttattagcct
    17761 cctcacttca tgacatttat aattctcgtc agaaaaaaga gaagaaggcc agaaaaataa
    17821 actatgttct gggggcggtc agcattgcca gtttctgcat cacgctgttc caggcctgtt
    17881 caaataaatt cattccatcc tgaatgattt attcctgttt attaaaacac ggcatcacta
    17941 cacttactgt cttatatcat tggcgctgac aacgttgccc cccatggatc tatgacgtcg
    18001 tcaaggccat cacgatgttc attgttttcg tcaacattgt tgttattccg gcgctggcat
    18061 tacccagaca ctgacgcgca ctgaccgtaa gggcgtggct tgtctgccga caccctgcca
    18121 caaaacaagt tttgcatcag ggtgtccggc ctctgtggac tataaatatt tcttgaagga
    18181 tgccattgtc acggtgacag ccatacccag cagcatggcc gccggccata tcagcagatt
    18241 cgggtacacg gccgtcggcc aggaaagata gagaatacag ggaatatata tcgccggttt
    18301 aatggacttt ttcgcatggt gataaacaaa gctggattca tacgcggcac cataccggcg
    18361 cagatcacgt ctgcccagcc cctcaaccac ggccactagg atcaccatca cgaacagtgg
    18421 cacggacagc acgagaataa tcaccctgac catcgtcacc accgtcacat aaaccgttgc
    18481 cagcaggtaa tcatgaagat gatatgccag ccactggctc agctctccgc tgaactgcga
    18541 cacactacgc cccaccgccg ttaccgcctg ctgcggctgc agccatgact gcataccact
    18601 ttccacaaac agccactgat acgccacgga aatccagccc ggtaccgtca ctgacggcgt
    18661 ggacagcagc aggctgcgcg tgaactccgt cgacagccag cccagctctg tattcatgac
    18721 ggcacgactg tgtgccgccc cctcatcagg ccagaaaaac gtcatacctg catactcaat
    18781 cagcagactc agtaacagtg aggagaggat cagccccacc agagcccatg gccagcccca
    18841 caccagattg tacagcaacc catgctgacg gggagggcgg gactgcgggc tgcgttctgc
    18901 atcacgacgt gcttccgcca tggtcacctc cctcctccgt cgctatcgcc tgtactgaag
    18961 gtggagcatt ccaccatcct tcaccggagt gataattctg tcgcatcgcc tccgccaccc
    19021 gggaaatact ctccggcatc agcacatcat ccgtatcacc tgccggcagc ggcatccgga
    19081 tcttccataa ctgtccacct tccagcaggg caaatgcctg ccccttgggc agcgtgacaa
    19141 tatctgcggg ctccagcagg gggactttca ccgtcccgac gcgatcctgg gtactggagg
    19201 tgaaatcctg accggctgac acatctgccg tatcctgatg accggagacc agcgtcttcg
    19261 tgtaaatctc cacctggggc agttgtgtgg tcagcaattc cgccgtccgg ttctccctga
    19321 cgcgcagcat gatcatgttg ttaaagttcc cctgaacctg cgccgttttg gccgcattac
    19381 caatacgcgc ttcaatatcc gatgacgtct gggtgtacgc ggtcacctgc attccggcgc
    19441 cgcccccctt gttgatgagc ggaatgaatt catcgcccat cagttcgtta aactcatcac
    19501 agtgcaggtt caccacattt ttacccgccc cgccagccgg cagaccggca tgcataccgt
    19561 gtttgtatat atgccctgcc acactcacca ggtccgcaaa catcgaattt cccacggcac
    19621 tggccacctc actgtcactg agcgcatcga ggccgatata aaccaccgcc tttttgcgga
    19681 tcacctgttc ccagtcaaaa atggggcgcg catcgctcat atccagatag tccggcgcca
    19741 gcagctctgc aatcctgccg gtggtgagtt tttccagtaa cggcagcagg gaagccacga
    19801 ttttgtcaaa atacgtacgg tcatagcgga cagcactgcg cagtccatcc agaatcggat
    19861 cgtatatttt tttaccttcc tcactgctca gtgccatttc aatcgaccag atccgcaccg
    19921 catccggctg gttctgcatg gtcctgggca catcttcctc gctgagaaca ctctggttat
    19981 tcgcgataat ctgctccagt ccgggcagac gctcacggat caccttcccc gcatagcgaa
    20041 tgtagaggtc cgcgatgttg ttcacatacc gcataatcag catatagtcc ggacgctcac
    20101 cgagggccac cagggcgcgg gcgatgatat tgacaaagcg ccaggcaaac tcccggaaag
    20161 ccgccgaatt accttcaccg gacaactgac cggcaacacg ggaagccact tccgatacac
    20221 gaccaaagcg tcccaccgca ttgtaacggg ctgaaatttc aggccagcca agatgaaaga
    20281 tatacaactc atccccccgc ccggcccggt gcgcttcagc ccagacccgc agcagcagat
    20341 ctgcatcccc cttgggatca aaaacgatgg tcacttctcc ccgccggata tcctgggtga
    20401 ccagcagctc tgccagacgg gttttaccca cacgcgtggt gccgtagaca atggtgtgcc
    20461 ccacacgttc acccagtgcc agtgtcacat cctgctcatc cggttcaata ccgtgcaggg
    20521 ccggattccc gcccaccggt ggcaggggac gaaccgggtt cagcggagaa tcacgacgaa
    20581 gccactgccc cagcccgggc agccggtgtt ccgttttcat ttcaagccag cgcgcagccc
    20641 ggtaaaatcc tcccggcagc agataatgcg ccacctccgg ccgcagcgta tcctgcagac
    20701 gctgggtatg cttctgtgtc cagcgaaatc cccgccccag aaacagtcgc tgacggctga
    20761 caggaatttg ccgcgtgctc atcacatagc ggggtaaccg acgcagattg cgtcggtagc
    20821 ggatgatttt catgccctgg cggaaacgga cagccgccag aacgccaaat ccggcagctg
    20881 tgacataact gaccgaaggc gcaagggcca ccgcccaggg ggctgtcaca cagacaccgg
    20941 ccgccgttgc cgcaaccacg gccgtattga actccacagc aggcctcagt aacgcctcaa
    21001 taacataacg gttactcatg tccgcgtcct cgctgtaaaa gacgcagcca ccatgagcgg
    21061 gggatatctg tctccggtga cgctgacaac gcctcagctc tgttctgcaa tctcctcacc
    21121 tcccgccatg cgtctgctgc attcagtaac aatgccttat actccgcaga cgcatgtccg
    21181 ccagcccgtc cgtcatccac tgtcagctga gcctctgttc tgaaacggtc cactttccag
    21241 caggccaggt cttcgcggga aacactctgc cgccgggtct gcagacgaag atacacacca
    21301 tgccgggaga gaacatcagc cagattgtga atttcccgac aggcagacca gaacgcccgg
    21361 tctgcgtgat aaatgctctg ggccacgttg tgaagctgtg tgcgctcctc tccctgcagg
    21421 tgaaacagaa agcgggccag acaactgcgg tgattaccat cagcactgac ataccagtga
    21481 atgccatccg tgctgacaaa actcatcccg gcatgcagtt gttctgcctg cgtgtaatac
    21541 cccgggttct tctccaccaa cgggatattg atatccatac gcttaccgcg ctccagcaac
    21601 tccagccagc tcattccggc atactgtgga tggtctgtcc ccacaatgcg gaacacattg
    21661 atgctgccct gccccgtcca gtagtgttcc gtaataaaat ccgacattgc cgcaaactct
    21721 gccgcatccc atgcccgtaa aggttcattc gcccattgcg gcatattcct ctgcacctgt
    21781 tcagtaaatg aaacgttcat ccccgctctc ctgtcatctt cgcttttctc cggtgagttc
    21841 accggtcagt aatcgcccgt tcagcagttt cagcagacgc tgtccgctaa ccagttcaat
    21901 atccggccac agcacaagcc gtgcccggct ggccggtccc gtacgcccgg tgtgaacaaa
    21961 aaatccacga caaccttcac gacgcagtaa ttcaccaaaa tcatcgatat gtgacggtcg
    22021 gatggtgcgg ccatagcgtt tggcctgaat cagccaccgc tcgccgtcaa tcagcacctg
    22081 tccgtccagc cctccgtcgc cgctgtagcg ggaattgcgg ataacccgca ttcccctgcg
    22141 ttcaaacgcc agaagcagca gttcttcaaa cacatacggg ttaatttttc gcaggtattt
    22201 cacgcctgca ccgccctccg gcaacgacga cagttgcgcc agcacacgct cagccttgct
    22261 ccggtaatgg cggtgacgcc actcactggc gcgttcacga cgccggcggc gaccacacag
    22321 gacggaaaaa acaggaacaa cacagccagg acacacagca gaacgccgta cggtatctta
    22381 tccggcacaa tatccgtcat ttgccgcagc atcacggtgt caccaccccg gatattccgc
    22441 tgtccgttat cagtaccggc cagtgattca gctgcagacg gcgtgccagc gctgaccccg
    22501 atgatggcac catcatcacg ccgggcgcca gctcacgcag cgttctcagg ctgttttcat
    22561 ccgccacgct gacaaccata ccgacagcct gccgctcacg gagagcgccc gcattcgcct
    22621 gcagccactg acgggaaagc gcatcatctc ccaccagaaa cagcgccccg atccccggca
    22681 actgcagtgg tcgcggcgtc acctcacccg gcgtcatttc ctgcgtggtc acgggcagca
    22741 acagcgtctc tccctgcgcc ggcagaggcg ccgtaaccgg gggaggcgta ctgctcccct
    22801 cctggcggtt gattgcctca taaaacacgg aggcatcact gcctccaagg tccgcaataa
    22861 ccctgagttc cgcgccagcc tgagccgcgc acagcagcag gggccacact gccgtcatta
    22921 cgtatcgttt cacaggtatc actcccgggg ttctgtccag atataacggg gatgcatggc
    22981 ggtcaggccg tcatgctgct ctgcggtctt caccgcatca cgatgaagac gctcaagatg
    23041 ttgtctgacc actgcggtgt agcgtactgc cggagcacca cctgccggat aatgataaca
    23101 accggcagcc tgcagccagc tgacaggatg acgacgacgg cattctctca ggatggtcgc
    23161 tgcggtgttc agactgatgt atggatcaaa tgccgcccat gtggaggaga aacggtgccc
    23221 gttccagccc agattgacct gagcaattcc cacgtcaatc cgccgggccg gataccgtgt
    23281 cagaaaaacc tgaagtgcct gccaggcttc caggcgactg gcatagcggt agcccttacc
    23341 cgccacattg atggtccacg gccagggacg tgcaacggac ggaaggcgga aatccgcatc
    23401 ccgttttttc atcacaccgg caatcgcaga gggcgccatc accgtctctg tcagagagac
    23461 ggcatagagc acttctgcag gcacaccatg cacggcagcc acgcgcacat agccctcagg
    23521 gatatcatgc gcccccgccc ggcagagact gctgacagcc atgctcaaca gcgccattgt
    23581 cagaaggctg ccacgcgcca tccgtccgct ccctgctgca gtacaaccgg cattctgcct
    23641 tcaccaaaac gcagccagcg accggcatca tgattcaggg taatatggcg gcttcgcacc
    23701 cggtccacag ggatatcgtg ctccagtgcc cactgccgca gcagattgtc actgccctga
    23761 ctgtcaacca gataaatatc cacctgccgc cccgaagaca gcactgatgc cagtcgtgca
    23821 tcacaggtgc cacagtcttt cgctttcaca aacaacgcca gccgtccgcc gttgtcgttc
    23881 agcagcccgc cccctttccc caggctgacc ggcataacgc ccggataaag gcgcttccag
    23941 gccgcattga cttcccgctg aaatgccagt tccttctcag tccgctcata ttcggccttc
    24001 acccatttct cagccagctg ccggcgttcc gcatccgtcc gtgcttcaat ccccagcacc
    24061 gtcagcggat ccagaccagg ggactgcatc ccccgcggtc cctccatcag agcccggtat
    24121 cgacggtaat cctcctccgt gacaccccac tgagcggcct gttgctgcag cgcctgtgtg
    24181 gtggtactga cttgcgaaga ggcctgcgtg cgtgtgccct cgggcgcact ctcccgtaca
    24241 gcagccaccg tctgcaggga aatgagcagc atgagcaccg gtgacagatt cttcattttc
    24301 atttcatcac tccaccatga cccggacctg acgtccgttc acccggaacg aggcttctcc
    24361 ccgtccggca cgctcaagca tccagccggc cgtgctctcc ccttcaccca gcagcgtcac
    24421 atctgaaaga cgggtaaatc cacggggagc caccgcagca tatgtgcgtc cgccacgctg
    24481 ctccgtgcct gtcagcacaa acggtgcgct gcggctgata actgaaatgc cggaagagcc
    24541 ctttttctgg cgtggcgtga ccgccggtgt gctcttcacc gtgcccgttc ctttcgcaga
    24601 tgacggtgat accggggtga atggcgcctt ctccagcttc tccagtcgca ggtcatacgc
    24661 acgaagcgtt tctccctgct gcgccagcgt ggcctgcagt gatgccaggc tggcggccat
    24721 ctgaccagtg cgtgcatgct cttcctccgt cagtacaggt cgttgctcca gcaatgtcag
    24781 tcgctccgtc acgcttttca gctgtttttt ggtttcagcc agccgggacc tgagcggttc
    24841 aagctcactt cccgacaacg cgagagcatc tgaatacgtt ttcatttgcc cctccagtgc
    24901 ggcaatgcgt tcatcctgct ccccgctgta acgatagagg ccgcaaaccg ccagaatggt
    24961 cgcaaccaga acggtcagta caaatcccac ggaaagcatg ggtgacacac aggtccaccc
    25021 atcaagagga gatgaatgca ctgacggtaa tgtcttctgt tttttttcac tcatttttta
    25081 agctccgctc ctgcgcccct gacaccggcc ggtaccggcg ccagctggcc cggcggcaac
    25141 tgataacctt cccgcaggct gtgacagaca acccgctgca catcatcaac ttcaagttgc
    25201 cacgccggac cggccatcat ctgcagggcg gtacgcaacc tgagcggccc cgtctgatac
    25261 tgcactgccg gcaacgcctg acgataaagc accccgttgg ccggtccggt ggcacagagc
    25321 gtgtagcccg actgtttcag ggcataacgc atcgcatctg ccaccgtcgg cttcagggat
    25381 gccggaatac ggatgtcaat cacctgggaa aggggatccc gctgtaccgc atccggatga
    25441 gtgttaacca gcagatagcg gtcataacgc accacctcgg gcacagtcgt ccccgcgtac
    25501 atatcagggc tggccggacg gacattattg ctgaccgtca ccgacggtac cggaacagcc
    25561 gcatcccgct gcagcagttt ttgctgcccc tgacagccag tcagaagtac gcaggcggac
    25621 agcacggaaa gaagaaggga tgttttcatc aggaagttac ctctgcggtg aagtgataaa
    25681 gtcagaggtc aatttgcaga agaagtccag accggctcaa tgaacaactg atttccggtt
    25741 gacaaaaaaa acgcctgcac aaggcaggcg tggtgtaaaa gggaacagag tcttccggtt
    25801 attcagtcct gatttagcg
//
LOCUS       DAZFFE010000069        24824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFFE010000069 DAZFFE010000000
VERSION     DAZFFE010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.069"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..963)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHJ69_002984"
                     /pseudo
     CDS             complement(1..963)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHJ69_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
     gene            complement(1179..5060)
                     /gene="entF"
                     /locus_tag="ACHJ69_002985"
     CDS             complement(1179..5060)
                     /gene="entF"
                     /locus_tag="ACHJ69_002985"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO5015925.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDAQTFELPEIIDLRTNIDPHGTARALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPQLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQAQTHPLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQI
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTRLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCSTLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAEVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRVP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSTQRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DLQWSIIGIQSPRPHGPMQTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVAFDGKATLFVAERTLQEGMSPEQAWAPWIAEL
                     DIYRQDCAHVDIISPVAFEKIGPIIRATLNK"
     gene            complement(5057..5275)
                     /gene="ybdZ"
                     /locus_tag="ACHJ69_002986"
     CDS             complement(5057..5275)
                     /gene="ybdZ"
                     /locus_tag="ACHJ69_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO5015926.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQASCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(5278..6480)
                     /gene="fes"
                     /locus_tag="ACHJ69_002987"
     CDS             complement(5278..6480)
                     /gene="fes"
                     /locus_tag="ACHJ69_002987"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO5015927.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEGHS
                     TIKRVWVYITGMTDHHQNSQPRSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPALTSL
                     THRRQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPQVKTIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGHQEGVLIEQLKTGEVSAEG
                     LRIVLEAGVREPMIMQANQALCAQLHPLKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            6723..8963
                     /gene="fepA"
                     /locus_tag="ACHJ69_002988"
     CDS             6723..8963
                     /gene="fepA"
                     /locus_tag="ACHJ69_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO5015928.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKAAQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     KNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            9138..9758
                     /gene="entD"
                     /locus_tag="ACHJ69_002989"
     CDS             9138..9758
                     /gene="entD"
                     /locus_tag="ACHJ69_002989"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO5015929.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPASFREQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAGVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLAECGLTFSLALTLAFSAKESAFKACER
                     QTDADFLDYQIISRNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(9880..10033)
                     /gene="hokE"
                     /locus_tag="ACHJ69_002990"
                     /pseudo
     CDS             complement(9880..10033)
                     /gene="hokE"
                     /locus_tag="ACHJ69_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
     gene            10478..11596
                     /locus_tag="ACHJ69_002991"
     CDS             10478..11596
                     /locus_tag="ACHJ69_002991"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO5015930.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSTLIDVVKNQ
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRSLTEATLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGE"
     gene            11662..11910
                     /locus_tag="ACHJ69_002992"
     CDS             11662..11910
                     /locus_tag="ACHJ69_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO5015931.1"
                     /translation="MKHSLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFFLRFIWRRWFSLAD"
     gene            11975..12358
                     /locus_tag="ACHJ69_002993"
     CDS             11975..12358
                     /locus_tag="ACHJ69_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO5015932.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRLRPDSTVNQ"
     gene            12437..13090
                     /gene="nfsB"
                     /locus_tag="ACHJ69_002994"
     CDS             12437..13090
                     /gene="nfsB"
                     /locus_tag="ACHJ69_002994"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO5015933.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAADNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDEEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            13198..14445
                     /gene="ybdG"
                     /locus_tag="ACHJ69_002995"
     CDS             13198..14445
                     /gene="ybdG"
                     /locus_tag="ACHJ69_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO5015934.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(14602..15984)
                     /gene="pheP"
                     /locus_tag="ACHJ69_002996"
     CDS             complement(14602..15984)
                     /gene="pheP"
                     /locus_tag="ACHJ69_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO5015935.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRKVN"
     gene            complement(16086..19229)
                     /gene="cusA"
                     /locus_tag="ACHJ69_002997"
     CDS             complement(16086..19229)
                     /gene="cusA"
                     /locus_tag="ACHJ69_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO5015936.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDTMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVSEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(19241..20464)
                     /gene="cusB"
                     /locus_tag="ACHJ69_002998"
     CDS             complement(19241..20464)
                     /gene="cusB"
                     /locus_tag="ACHJ69_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO5015937.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKEEPPAEKTSTAERKVLF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFSQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(20480..20812)
                     /gene="cusF"
                     /locus_tag="ACHJ69_002999"
     CDS             complement(20480..20812)
                     /gene="cusF"
                     /locus_tag="ACHJ69_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO5015938.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KDVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(20836..22218)
                     /gene="cusC"
                     /locus_tag="ACHJ69_003000"
     CDS             complement(20836..22218)
                     /gene="cusC"
                     /locus_tag="ACHJ69_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO5015939.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMAALKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTRELSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLPSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            22375..23058
                     /gene="cusR"
                     /locus_tag="ACHJ69_003001"
     CDS             22375..23058
                     /gene="cusR"
                     /locus_tag="ACHJ69_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO5015940.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMSILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            23048..24490
                     /gene="cusS"
                     /locus_tag="ACHJ69_003002"
     CDS             23048..24490
                     /gene="cusS"
                     /locus_tag="ACHJ69_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO5015941.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLANSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQAGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLGDEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     TGETIVVRCQTVDHLVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIILPA"
     gene            24640..>24824
                     /locus_tag="ACHJ69_003003"
     CDS             24640..>24824
                     /locus_tag="ACHJ69_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015942.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYR"
BASE COUNT         6031 a         6708 c         6223 g         5862 t
ORIGIN      
        1 taaacggcgt aaaattcaca tcgttgatat ttgcttttgt taactgctcg acaagatact
       61 ggcgattacg taattcaccg ttcaattccg caacgtcagt aaccgctttt tcaatttcca
      121 gtttgcgttc aataccgtct gcaccgagag aaatagagaa atcggggtca tctttaacgg
      181 cctgaccatt actgtatacg ggctttttaa ttcctgccgc gttggcaatg tcgagtgaat
      241 aattgaggcg ctgaatgttt gcatcaagtt gatttttcgt tttaatgcga tcctgagcca
      301 gtttttcttt ttcaaactgg gttttgatct ccagtttatt acggacgttt tctatcgact
      361 ctttcaccac caacgcagag atataatcga tatacccgct caaaacggtc tgcgcctctt
      421 cactggttgg cgcggtaaaa cttagcgtcc aggaggtata cagtgacggt tcatcttttt
      481 tcttactggc attgtcatca accgctttca ttttttcgct caatgcgacg atagcgcgat
      541 gcaaatccag ttcgtcgatt ttcgcttctt ttaattgatc catcacataa ggtgatgagc
      601 gcaggtactc ttctagtaag ctaacagact gaaatttctt gataaacagg ttaaatactt
      661 ctgtacgatc aattttgata tccagatcca gcacacgaag cttagtgaat gttttctcca
      721 gctcttgcca ctgaacaggt tctggaggcg tgacaaccgc cgcgctggtc catttttgcg
      781 gcaggatgaa agagatcagc aatcctgcgc aggcaaacgc aaaaatgacc gccatgaccg
      841 tttttttgcc cgccataaaa cttcgattag attaagtaaa tcaatttcat tattactggg
      901 cgacgccaga ggatactcgg gaaaatgagt gtcacttccc tgtttaatat tcagtgatga
      961 catggggtat aacctgaaaa tgagtccaat aagagtaaat ctccacggcc cccactctac
     1021 cagttggtga gatttatccg aagctgaagt ttgtaaatta ctcccactga atgcaacatt
     1081 cactctattt cagagaaata cccaccaaat ttataaatta aacaaatgat aaacccataa
     1141 ttattttctg taattatggg tttatagata atataaattt acttatttag cgtcgcacga
     1201 ataatcggcc caattttttc aaacgccacc ggagagataa tatccacatg cgcacaatcc
     1261 tgacgataga tatccagctc agcaatccac ggtgcccagg cctgttcagg actcatacct
     1321 tcctgaagcg tgcgttcggc aacaaatagc gtcgctttac catcaaacgc tacgctatga
     1381 gctgtcgtca acaggcgcac agcatcagcg tagttgcctt caatggtggt aaacaactcc
     1441 gttgaagtac ttccctgctg tgccgccagg aaggcttcgc gctcgcggtt aatctccgcc
     1501 agcacttccg gatccagacc attggcttct ttttcctgcc agttttgcgt ttctggcggc
     1561 caagtatcca gcaagccaag aaatgccacc tgttcgccac gggcacgcag tcgcgccgca
     1621 atgccctgcg ccagcgtacc gccaagtgaa taacccagca ggtaatatgg gccgtgcggt
     1681 tgttgttcaa gtaaggttgc cagatgcgct tcgcagactt catccaggtt tgccgccgtc
     1741 tgcatggggc catgagggcg cggcgactga atgccgataa tcgaccattg tagatcgaga
     1801 taacgcgaga gcacgctgaa ctgccaggca aaaccggatg ccggatggaa acaaaacagc
     1861 gtcgggccat taccttcacg caacggcaga atggtttcga atcccatgcg ctgggtgctg
     1921 tcttcttcac catcaataat cgttgccagt ttggcgacag ttgacgcgac catcacctgc
     1981 cccggcgtca cctgacgagc aaactgtcga cttaactgcg ctgccagttt cattgccagt
     2041 agcgaatgac cgccaagcgc aaagaaatca gcatcggcat cctgcacgtc acaacccagc
     2101 aacgacgaga atgccgcggc gataatcgtt tcactgcccg ctttcggcac acgccccggc
     2161 gtttgtgcct tcagttcagg caacggtaag gctttgcgat ccagcttgcc gttggcgcta
     2221 agtggtaact gtggaagttg cagcagaacc accggtacca tatgtggtgg caatgtttct
     2281 cgaagctgcg cctgtaatgc gctggtatcc aacggcagac ctgattgcga caccagatag
     2341 cccaccaact gacgcgcatc accaccagtg gctgccgcct ggttaatcac acaggcgtgg
     2401 gtaacggctt gttcgacatc cggcagcgcc tgcatcacgc gatcaatttc gcccagttcg
     2461 atacgctgcc cgcgaatttt tagctgatcg tcactgcgcc cgaggtactc cactgcgccg
     2521 ttatccagcc agcgggcaac gtctccggta cggtacatcc gttcacctgg ggcaaaagga
     2581 tcggcaataa agcggctggc ggtcagatcg ggtcgtccaa gatacccctg cgccagttga
     2641 ataccggtga gatagagatc gcccgccaca cccggcggca ccggatgcat catcgcatcg
     2701 agaatgcgca ggcccgtatt ccacaccgga taaccaatcg gcacactgct gccgcgcacc
     2761 tctgccagtt cctcgccaaa agccggatac caactgacat ctaccgccgc ttccgtcggg
     2821 ccatatagat tatgcaacgg cgcgccggtt aactgttgcc attcgcggca taaatcagcc
     2881 ggtagggctt caccactaca gaaaacctgt ttcaacgtcg agcaattctg acgagcggtt
     2941 tgcggcgtca gcgatgcaac aaatgctgcc agcatcgacg gcacaaagtg cgtggtcgtt
     3001 acaccatatt cggcaaagaa ttgctgcata gcgagcgggt cgcggtgcgc ttccggttca
     3061 gccatcacca gtttagcccc ggcaataaac ggccagaaaa actcccacac cgagacatca
     3121 aaactgcacg gcgttttttg ggcaacgaca tcttcgcctg taagcgggta gtggttttgc
     3181 atccaaagca ggcggttaac gatagccgtc tgcccgacca ttaccccttt cggcctgccg
     3241 gtggagccag aggtaaagat gatataagcc gtgtggtgcg gttgtgaaag ctgcagcggc
     3301 gcactgccct gcggtgtaag cggggcgtta tagcaaaggc gggttaaatc tggaacatcg
     3361 gcaaagcgcg gcagttgatc gtcagtggtg attaacagcg acggacgcgc atcttccagc
     3421 atcattttca ggcgatcgtc cggatagccg gtatccagcg gtaaccaggc cgcaccagcc
     3481 tcaacaattg catgtagcgc gagagtcaaa aagaccgagc gcggtagagc caccgccacg
     3541 ctgtcgcccg gtttaacgcc gcgctcacgc agcagattcg ccagcgccac cacctgctcg
     3601 cgcatttccc gatagctgaa ctggtaacgc gcatctgcca gcgccggagc atccggtgtt
     3661 tttgccgctt gttctgccac cagcgcgctc agcgtggttt ctggaatctc aatctgagtg
     3721 gcgttgatct gcgccagctg cgcatactca cctggcagca taatatcgac atcgccgcac
     3781 aacagcgccg gatccgcggc gaactgggca atcagcattt tcaggcgttc agcgtgttga
     3841 attaacgttg gctcatcgta acgctgttta ttggcgagga tttcaatact caaatctccg
     3901 tgttcatccg ggaacagggc cagttcaagg tcattaaccg gaccggttgc caggggatgg
     3961 gtttgcgcct gaacaccagg aatatccagt tggtaatcaa ataccttgat attgagtacc
     4021 ggaccgaaca gcggttcctc tcctgccgct cgcccactgt cacggacaat ttgttcggcg
     4081 tcgtagcgtt gatggcggcg catttttttc agttgtgctg ccagtcgggt tgccagttgc
     4141 ggcagcgttt cttgcgccgc aatgtgaata cccaacggta aaacgttgag cacgggtccg
     4201 gtagccgtca gcgccgccga gcccagtcga cgcataaaga taaatccggc agcgtagtcc
     4261 atgcggttgc acaatcgccc cagccacaag gctgccaacg caagtgctaa atcggtacgc
     4321 tgcacacctg aaagttgcgt agccagctgg cggaattccc cgtcggtaaa ttccagtttc
     4381 aggcgcaaaa tatctgccga agcgctgcgc cccggtaaag gtgccggaga aagtgacgcg
     4441 ggcggtggca gttgacgacg ctgttccgcc cagaatgccg catcacgctg ccaggcttca
     4501 ctttcgcggt actgctgata ctcttccact acatcagcga aaggcgtaaa cggcgaagca
     4561 ggcgttggtt cgccacgcag caatgcgcag taaatattgg cgatctggcg tgtaatggcc
     4621 gggaaactga agccatcgac cagcaaatgg tgataacgct gataccagta ccagcggtta
     4681 tccgccacct gtatcagctg atgaaagacc agtggtttac cactatcgac gcgcagatct
     4741 tgttgcaaat ccgcctgcat taatgcccgc gcagtaccgt gcggatcaat gttggttcgc
     4801 aggtcgataa tttctggcag ttcaaacgtc tgcgcatcat cgacccactg ccagacttcg
     4861 ccgttatctt ccgtaaaacg catccgcaac gtatctgctt gcgctagtcc ggcaaccacc
     4921 gcgcgggcca gtagtggcgc atcaacctct ccggttaact caacgtaatg cgccacgctc
     4981 caggcggagg gtaaatccga cagtttttct gccatccaga tgccgggctg tgcggcgacc
     5041 aaaggtaaat gctggctcat tgtgcctcct gcaactgggt gaaattcgcc ggtgtcagag
     5101 tacgccagtg ggcttccagc cactgctggc aggacgcctg tgactgcggc tgacacacaa
     5161 cgtcccagcc tgccggtaag gcgcattgtt gcggccacag actgaattgc ccctgcgcat
     5221 tgcgcaaaat gtaaaacgct ccctgtggat catcgaaggg attactgaat gccatattca
     5281 actcctgtca tggaaaagtg gttgccagag gtcgattagc ccctgcatca agccaccgcg
     5341 ccaacaaagc gcatcatgtc cgccgtcaac ctgacgccag aaaatggatt cttttagggg
     5401 gtgtaattgc gcacacagcg cctgattggc ctgcataatc atcggctcgc gaacacccgc
     5461 ttccagcaca atgcgcagac cttcggcgct aacttcacca gtttttagct gttcaattaa
     5521 cacgccctct tggtgcccgc ccctatgcgg ccaccagtac gagcctgact ggcttaatac
     5581 acagccaaag cgttcaggcc agtgcagtcc ggcatacagc gcggaaagcc caccaaaact
     5641 ctgtccggcg accacggtgc gatcggcgcg atcgctaaaa ggggcaatag ttttcacctg
     5701 gggtaataac tcttgctgta ctgccagcca gaaatccgca ttacacggca gttcgtgggc
     5761 gcggtgcgtg gtgtcgatag cgtcgatcaa cacatacacg gcgggaggaa gttgccgacg
     5821 atgggtcagc gaagtcagcg cgggccagac gggcatactc tgcgcccaaa attcgccatc
     5881 aagcaaaact gccagcgggc gttcttctgc tgttgcatcg ccggtggtaa aaatccatac
     5941 acggcgtgaa tttttcaacc gttcactttt ccagataatt tctttggcag gcgtttctgg
     6001 cgcttgcgga caatcccatc ccggttgcag aggcgcttgc ggcatttcga gtgcagaaac
     6061 agcgtgcccg cgcccgcctt tccagctttg tggattcagc ggatcggcta tcgcctgggg
     6121 taatagtttt cgccagcctt cgcgtaattc gaggcgatcg ggggatggtg cagaaaaaat
     6181 gtcatcgcgt tcggtaggaa taaagcagta gctgccgcgc cagttggcat tgagttgtgt
     6241 cgtccactgc caaacatcgg ttcctgcaat tcgctgcatc gaccggggct ggctgttctg
     6301 atggtgatcg gtcatgccag tgatatagac ccatacgcgc tttatcgtcg agtggccttc
     6361 agaaccttgt ggatcacgcc accaaaaagt gacttcaaac atttcgtcat tcagacgttg
     6421 ccattccggg ccatgtttcg actgccacca gctctcactt cctactttta acgccgtcac
     6481 accataaccc catgtttact gtgcaatttt ttattgattg cagaaatata ttgataatat
     6541 tattgataac tatttgcatt tgcaatagcg taatggcgcg ccgtgggaag cgcggacatt
     6601 aattaatcag ctgcactgcg tgtctttcag gatcaaaggt tttcgcggta gcgggatgcg
     6661 tcgtgttgat gacgaccatg cccgacagtt gcaattcgtg gcaaaaatgc aggaataaaa
     6721 caatgaacaa gaagattcat tccctggcct tgttggtcaa tctggggatt tatggggtag
     6781 cacaggcaca agagccgacc gatactcctg tttcacatga cgatactatt gttgttaccg
     6841 ccgccgagca gaacttgcag gcgcccggcg tttcgaccat taccgcagat gaaatccgca
     6901 aaaacccggt tgcccgcgat gtatcggaga tcattcgtac catgcctggc gttaacctga
     6961 ccggtaattc caccagtggt cagcgtggta ataaccgcca gattgatatt cgcggcatgg
     7021 gtccggaaaa cacgctgatt ttgattgacg gcaagccggt aagcagccgt aactcagtgc
     7081 gtcagggctg gcgtggcgag cgcgataccc gtggtgatac ctcctgggtg ccgcctgaaa
     7141 tgattgaacg tattgaagtt ctacgtggtc cggcagctgc gcgttatggc aacggcgcgg
     7201 cgggcggcgt ggttaacatc attaccaaaa aaggcagcgg tgagtggcac ggctcctggg
     7261 atgcttattt caatgcgcca gaacataaag aggaaggtgc caccaaacgc accaacttca
     7321 gcctgaccgg tccgctgggc gacgaattca gcttccgctt gtatggcaac ctcgacaaaa
     7381 cccaggctga cgcgtgggat atcaaccagg gccatcagtc cgcgcgtgcc ggaacgtatg
     7441 ccacgacgtt accagccggg cgcgaagggg tgatcaataa agatattaac ggcgtggtgc
     7501 gctgggactt cgcgcctctg caatcactcg aactggaagc gggctacagc cgccagggta
     7561 acctgtatgc gggtgatacg cagaacacca actctgacgc ttacactcgc tcgaaatatg
     7621 gcgatgaaac caaccgcctg tatcgccaga actactcgct gacctggaac ggcggctggg
     7681 ataacggcgt gaccaccagc aactgggtgc agtacgaaca cacccgtaac tcgcgtattc
     7741 cggaaggtct ggcgggcggt accgaaggga aatttaacga aaaagcggca caggattttg
     7801 tcgatatcga tcttgatgac gtgatgctgc acagcgaagt taacctgccg attgatttcc
     7861 tcgtaaacca aacgctgacg ctgggtacag agtggaatca gcaacggatg aaggacttga
     7921 gttccaacac ccaggcacta accggaacga ataccggcgg tgctattgat ggcgtgagtg
     7981 ccaccgaccg tagcccgtat tcaaaagcag aaattttctc gctgtttgcc gaaaacaata
     8041 tggagctgac tgacagcacc atcgtaacgc cggggctgcg tttcgatcat cacagtattg
     8101 tcggcaataa ctggagcccg gcgctgaaca tttcgcaagg tttaggcgat gacttcacgc
     8161 tgaaaatggg catcgcccgc gcctataaag cgccgagcct gtaccagact aacccaaact
     8221 acattctcta cagtaaaggt cagggctgct atgccagcgc gggcggctgc tatctgcaag
     8281 gtaacgatga cctgaaagca gaaaccagca tcaacaagga gattggtctg gagttcaaac
     8341 gcgacggttg gctggcgggc gtgacctggt tccgtaacga ttatcgcaat aagattgaag
     8401 cgggctatgt agctgtaggg aaaaacgcag tcggcaccga tctctatcag tgggataacg
     8461 tgccgaaagc ggtggttgaa ggtctggaag gatcgttaaa cgtaccggtt agcgaaacgg
     8521 tgatgtggac caataacatc acttatatgc tgaagagtga aaacaaaacc acgggcgacc
     8581 gtttgtcgat catcccagag tatacgttga actcaacgct gagctggcag gcacgggaag
     8641 atttgtcgat gcaaacgacc ttcacctggt acggcaaaca gcagccgaag aagtacaact
     8701 ataaaggtca gccagcggtt ggaccggaaa ccaaagaaat cagtccgtac agcattgttg
     8761 gcctgagcgc gacctgggat gtgaccaaga atgtcagtct gaccggcggc gtggacaacc
     8821 tgttcgacaa acgtttgtgg cgtgcgggta atgcccagac cacgggcgat ctggcaggag
     8881 ccaactatat cgcaggtgcc ggggcgtata cctataacga gccgggacgt acgtggtata
     8941 tgagcattaa tactcacttc tgaagctaac gtcagattgt tgacaaagtg cgcgtagttc
     9001 atgccggatg cggcgtgaac gccttatccg gcctacaaaa atcttgccaa ttcaatatat
     9061 tgcaggacca cgtaggcctg ataagcgtag cgcatcaggc agttttgcgt ttgtcatcat
     9121 tctctaatat ggttgacatg aaaactacgc atacctccct cccctttgcc ggacatacgc
     9181 tgcattttgt tgagttcgat ccggcgagtt ttcgcgagca ggatttactc tggctgccgc
     9241 actacgcaca acttcaacac gctggacgta aacgtaaaac agagcattta gccgggcgga
     9301 tcgctgcaat ttatgcgctg cgggaatatg gctataaatg tgtgcctgca atcggcgagc
     9361 tacgccaacc tgtgtggcct gcgggggtat acggcagcat tagtcactgt gggactacgg
     9421 cattagccgt ggtatctcgt caaccgattg gcattgatat cgaagagatt ttctctgtac
     9481 aaaccgcaag agaattgaca gacaacatta ttacaccagc agaacacgag cgactcgcag
     9541 aatgcggttt aaccttttct ctggcgctga cactggcatt ttccgccaaa gagagcgcat
     9601 ttaaggcatg tgaaagacaa actgatgcag attttctgga ctatcagata attagccgga
     9661 ataaacaaca ggtcataatt catcgtgaga atgagatgtt tgctgtgcac tggcagataa
     9721 aagaaaaaat agtcataacg ctgtgccaac acgattaatt gacaacatct ggtacgattc
     9781 gcccgcagcc atctctgact gcgggcgaaa gtgtaaagca ggtgccttac catcctgacc
     9841 tgacaaccgg atatgcgggg attactcccc gcacaacggc tacttcttcg gttcgtaagc
     9901 gagaacagcc ttaaactcaa tattacgttc ctttacggta aactcacaca gcgagtctcc
     9961 gacctagaag cgtaaatccc agcaccgtta aacacagcac tatgactgcc acaagggcat
    10021 atttcgtcag catgtttata ttgcctccat gcgaagaagg gactaccatc caaattgttc
    10081 aggtttgggt aggtagcccc agggttgata gaaatatcgt cctggggctt catctttcca
    10141 gacctcagag taaacctgaa accggaaagc ttcaggcacc cgcagttatc ttacccttcc
    10201 ttagcaaaat agctgtttat ttatacagtg agtcctgttt cggacttctc atttaatcaa
    10261 ctgaagctga acccgttttt tctgcatgcc tcaatcgtca aaattcttcg ccgccagcca
    10321 gctctgccat aacttgtcac attgcaaaag tagccaaatt gcttcagtca caatcagttc
    10381 tccatcacca aaaaaagaaa cattccagaa cttccgcatc tcacccagcc gctcccactt
    10441 ctatactgaa tagaaaacgc caacataaga gaaacctatg ccattacccg attttcatgt
    10501 ttctgaacct tttaccctcg gtattgaact ggaaatgcag gtggttaatc cgccgggcta
    10561 tgacttaagc caggactctt caacgctgat tgacgtggtt aaaaatcaga tcacggccgg
    10621 agaggtaaag cacgatatca ccgaaagtat gctggagctg gcgacggatg tttgccgtga
    10681 tatcaaccag gctgccgggc aattttcagc gatgcagaaa gtcgtattgc aggcagcggc
    10741 agaccatcat ctggaaattt gcggcggtgg cacgcatccg tttcagaaat ggcagcgtca
    10801 ggaggtatgc gataacgaac gctatcaacg aacgctggaa aactttggct atctcatcca
    10861 gcaggcgacc gtttttggtc agcatgtcca tgttggctgc gccagtggcg atgacgccat
    10921 ttatttgctg cacggcttgt cacggtttgt gccgcacttt atcgcccttt ccgccgcgtc
    10981 gccatatatg cagggaacgg atacgcgttt tgcctcctca cgaccgaata ttttttccgc
    11041 ctttcccgat aatggcccaa tgccgtgggt cagtaactgg caacaatttg aagccctgtt
    11101 tcgctgcctg agttacacca cgatgatcga cagcattaaa gatctgcact gggatattcg
    11161 ccccagcccc cattttggta cggtggaagt tcgggtgatg gataccccgt taacccttag
    11221 ccacgcggta aatatggcgg gattaattca ggctaccgcc cactggttac tgacggaacg
    11281 cccgtttaaa catcaggaga aagattacct gctgtataaa ttcaaccgtt tccaggcctg
    11341 tcgctatggg ctggaaggcg tcattaccga tccgcatact ggcgatcgtc ggtcactaac
    11401 ggaagccacc ttgcgtttac tggaaaaaat cgccccttct gcacataaaa ttggcgcatc
    11461 gagcgcaatt gaagcactgc accgtcaggt cgtcagtggt ctgaatgaag cgcagctaat
    11521 gcgtgatttc gtcgccgatg gtggctcgct aattgggctg gtgaaaaagc attgtgagat
    11581 ctgggctgga gaataagtcg ggaattgcct tacagaagaa tatccctgac aatggtgttt
    11641 taacttacat ttaacaatgt tatgaaacac tctttagaaa ccttgaccac cgcagcaggc
    11701 attttgctga tggctttcct ctcttgcctg ctgctgcccg cccccgcact ggggctaacg
    11761 ctggcacaaa aactggtgac cacgttccat ctgatggatc ttagccagct ttacacctta
    11821 ttgttttgtc tgtggttttt agtgctgggc gctattgagt atttttttct acgctttatc
    11881 tggcgacgct ggttctcgct ggcggattaa acgtcgatta tcgccgaact tcgcggcata
    11941 ctttgcttat tctccttcgc ctaaaggaac gtttatggat aagcaatcac tgcacgaaac
    12001 ggcgaaacgc ctggctcttg agttaccctt tgtcgagctt tgctggcctt ttggcccgga
    12061 gttcgatgtt tttaaaattg gcggcaagat ttttatgctg tcgtcggagc tgcgcggcgt
    12121 cccctttatc aatctgaagt ccgatccaca aaaatccctg ctaaatcagc aaatataccc
    12181 aagcatcaag ccagggtatc acatgaataa aaagcactgg atttcggtgt atcccggcga
    12241 ggaaatctcc gaagcgttac ttcgcgatct gatcaacgat tcgtggaatc tggtggttga
    12301 tggtctggct aaacgcgatc aaaaacgact gcgtcctgac tcaaccgtaa atcaatagcg
    12361 catttttctc gcttaccatt tcatctcgat ccttgtaatc tgctggcacg caaaattact
    12421 ttcacatgga gtctttatgg atatcatttc tgtcgcctta aagcgtcatt ccactaaggc
    12481 atttgatgcc agcaaaaaac ttaccccgga acaggccgag cagatcaaaa ctctactgca
    12541 atacagccca tccagcacca actcccagcc gtggcatttt attgttgcca gcacggaaga
    12601 aggtaaagcg cgtgttgcca aatccgctgc cgataattac gtgttcaacg agcgtaaaat
    12661 gcttgatgcc tcgcacgtcg tggtgttctg tgcaaaaacc gcgatggacg atgcctggct
    12721 gaagctggtt gttgaccagg aagatgccga tggccgcttt gccacgccgg aagcgaaagc
    12781 cgcgaacgat aaaggtcgca agttcttcgc cgatatgcac cgtaaagatc tgcatgatga
    12841 tgcagagtgg atggcaaaac aggtttatct caacgtcggt aacttcctgc tgggcgtggc
    12901 ggcgctgggt ctggacgcgg tgcccatcga aggttttgac gccgccatcc tcgatgaaga
    12961 atttggtctg aaagagaaag gctacaccag tctggtggtt gttccggtgg gtcatcacag
    13021 cgttgaagat tttaacgcta cgctgccgaa atctcgtctg ccgcaaaaca ttaccttaac
    13081 cgaagtgtaa ttctcccttg ccgggcatct gcccggctat ttcccctcag attctcctga
    13141 tttgcataac cctgtttcag ccgtcatcat aggctgctgt tgtataaagg agacgttatg
    13201 caggatttaa tatcccaggt tgaagattta gcgggtattg agattgatca caccacctcg
    13261 atggtgatga ttttcggtat tatttttctg accgccgtcg tggtgcatat tattttgcat
    13321 tgggtggtac tgcggacctt cgaaaaacgc gccatcgcca gttcacggct atggttgcaa
    13381 atcattaccc agaataaact cttccatcgt ttagctttta ccctgcaggg gattatcgtc
    13441 aatatccagg cggtattctg gctacaaaaa ggcaccgaag cggcagatat tctgactacc
    13501 tgcgcgcagt tgtggatcat gatgtatgcg ctgctttcag tcttctcgtt gctggatgtt
    13561 attttgaatc tggcgcagaa attcccggcg gcatctcagt taccgctgaa agggatattt
    13621 caggggatta aactgatcgg cgcgattctg gtcggcattt tgatgatctc gctgctgatt
    13681 ggtcagtcgc cagcgattct gatcagcggt cttggtgcaa tggctgccgt gctaatgttg
    13741 gtatttaaag atccgattct tggtctggtg gcaggtattc agctttccgc gaacgatatg
    13801 ctgaaactgg gcgactggct ggagatgccg aaatacggcg cggatggcgc ggtgatcgat
    13861 attgggttaa ccaccgtcaa agtgcgtaac tgggacaata ccattaccac tattcccacc
    13921 tggtctctgg tttctgactc ctttaaaaac tggagcggga tgtcagcatc tggcgggcga
    13981 cgtattaagc gcagtatcag tattgatgtc accagtattc gttttcttga tgaagacgaa
    14041 atgcaacgtc tgaataaagc gcatttgtta aagccttatt taaccagccg ccatcaggaa
    14101 attaatgagt ggaatcgcca gcaaggttct acggagtcgg tattaaacct gcgccgaatg
    14161 accaatattg gaacctttcg tgcctatctg aacgaatatc tgcggaatca tccgcggatt
    14221 cgcaaagata tgaccttaat ggtacgccaa ctggctcctg gtgataacgg tttaccgctt
    14281 gagatctatg cgtttaccaa caccgtggtg tggctggaat atgaaagcat tcaggctgat
    14341 atattcgatc acatatttgc gattgtcgaa gagtttggtc tgcgacttca tcagtcgcca
    14401 accggcaatg atattcgctc tctggcgggt gcatttaagc agtaattaaa aaaaccgctc
    14461 tcatcgaatg gatgagagcg gtttcggatg gttgacatcg ttttgtcgga tgcggcgtga
    14521 acgccttatc cgacctacag aatcgttaca aattgtaggc ccgataagct tgcgcatcgg
    14581 gcaaatcagc ctcacatcag cttagttcac tttcctacgc aatgttttaa acgccacaaa
    14641 caggaataca atccacaccg gcagcagaat cgctgacaag cgcatatcat ccatcgtgca
    14701 catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga acggatagag
    14761 cagcgcctta aattgtgttt cacgcccctg acgtcgcatc gccgcgcgaa aacgcagatg
    14821 cgccaggcag atcataatcc agttcaacag cagcgttgct accaccagcg ccatcagcag
    14881 accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag tgatcgctcc
    14941 ggaaagcatc agcgagttaa tcggcacgcc gcgacggctg acgcgagtca aaaactttgg
    15001 cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa ccccgctgtt
    15061 atacaccgac agcgatgcca ccagaatgac gaagttcagc gcagaagcca ccacattgct
    15121 gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt ccacccacgg
    15181 gtagagcgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc gatacaccac
    15241 ctgattcact gctttaggga tgcttttttc cggatcgcgc gcttcagccg cagtaatccc
    15301 aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa tcagtccatt
    15361 ccagccggtg gcaaagaaac cgccgtagcg ccagaggtta tcgatactgg ctttctcacc
    15421 gccgtgccca gaaaacagca gccacaggcc aaagccgatc ataccgatga tcgccagcac
    15481 tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca ccaggttaac
    15541 ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat ccgggaacca
    15601 gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca cgaacattac
    15661 ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat aggcaaagtg
    15721 ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccgagctggc gcataatcag
    15781 gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg ccatctgaat
    15841 cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg caatcagttg
    15901 aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg cagtatcttc
    15961 cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt tgaggggcac
    16021 cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa cggggtatcc
    16081 tgcttttatt tccgaacccg atgtcggtgc agccacatca gcttatacgc cgccgggata
    16141 ataaacagcg atagcaaagg cgcggtgatc atgccgccaa tcatcggcgc ggcaatccgg
    16201 ctcatcacct ctgaacctgc tcccgttccc cacagaatcg gcagcagacc agcgataatc
    16261 accgccaccg tcatcgcttt cgggcgcacg cgcaggaccg cgccgtgata caacgcctca
    16321 tccagcttct gctcgctgaa tgtttgcgga ttattcaacg acggctcggc ctctatggcg
    16381 tgacgtaaat acatcagcat caccacgcca aattcggcgg cgaccccggc aagggcaata
    16441 aaaccggtgc ccgtcgccac ggaaagatga aagcccatcc accacagcaa ccagatgccg
    16501 cccaccagcg caaacggtac gctgctgata atcagcaacg cttcgctcac ccgacggaac
    16561 gccagataca acagcacgaa gatgatcatc agcgtcatcg gcaccatcag tttcagctta
    16621 tggttggcgc gctccagtag ctcgaactgc ccggagaatg ccacgctggt gcccggtttt
    16681 aactgcactt tttcagctat cgctttttgc agatcgtgaa ccaccgacac catgtcacga
    16741 tcgcgggcat cgatataaat ccagctcgtc gggcgcgcat tctcggtttt cagcatcgac
    16801 ggtccggtag agactttaac gtcggccacg tccgccagag tgatttgctg cttcatcggc
    16861 gtgaggatcg gcaactggcg cagtgcctgc gggctatcgc gccagctttg cggataacgc
    16921 agattaattg gataacgagc aatcccttcc accgtttcgc caaccatcgc cccgcccacc
    16981 gcagaagtca caaacaactg cacatccgcc accgtcatac cgtaacgcgc ggctttttca
    17041 cggttaatct caacgttgat atagcgccca ccttccagac gctcagcaag cgcagaagcc
    17101 acgcctggca ccgttcgcgc cacttcttca atttgctccg ccatcgtgtc gatatccgcc
    17161 agcacagtgc cggaaacttt aatgccgata gggcttttaa tgccagtcga gagcatatcg
    17221 atacggttac gaattggcgg cacccacaga ttcgccagcc ccggcagacg cacggtgtta
    17281 tccagttcct caatgatttt gtccatcgtc atgccaggcc gccactgatc ctgcggctta
    17341 agctggatag tcgtttctac catttccagc ggagcagaat cggtggcggt ttccgctttc
    17401 ccggttttgc caaatacccg cgccacttca ggtacgctca taatcagctt gtcggttttt
    17461 tgcagcatac tcgccgcctc cgctgcggaa atccccggca gcgtcgatgg catatacaac
    17521 aagtcgcctt cattgatctg cggtaaaaat tccccgccaa ctttattgag cggccagaga
    17581 accgtcagca ccgaaagcgc tgccaccaac agcgtggttt tcggccagtg cagtactttc
    17641 agcaacagcg gatgataaac acgaatcaaa aagcgattga gcgggttact gctttctggc
    17701 ggaattttgc cacggatcca gtagcccatc aggatcggga tcactacaat cgccagtagc
    17761 gccgcgcccg ccatcgcata ggttttggtg aacgccagcg ggccaaacag acgaccttcc
    17821 tgcccttcca gggtgaagat cgggataaac gacagcgtga taatcagcag actgataaac
    17881 agcgccggcc ccacttcaac agatgcatcg gtgatcacct gccagcgcgt tttattatcc
    17941 agcgtggcgt caggatgctg gtgctgccac tcttccagcc gtttatgcgc attctcgatc
    18001 atgacgatag cggcatcgac catcgccccg acggcaatcg caatgccacc caacgacata
    18061 atattggcgt tcagtccctg aaagtgcatg acaataaaag caatacacaa ccccagcggc
    18121 aacgaaataa tcgccaccag cgccgagcgc acatgccaga gaaacagcgc acagactacc
    18181 gccaccacaa taaactcttc cagcaacttg ccgctgaggt tgtcaatggc gcggtcaatg
    18241 agctggctgc gatcgtatgt tgtaactatc tccacgcctt ccggcagact acttttcagc
    18301 gtttccagtt tgtccttcac ggcggcgatc acttctcggg cgtttttgcc ggaacgcagg
    18361 atcaccaccc cgcccgccac ttcgccttcg ccgttcagtt cggcaatgcc ccggcgcatc
    18421 tccgggccaa cctggacctt cgcaacatca cgcagataaa cgggcacgcc attttcactg
    18481 gcttttaaaa cgatgtgatt aaagtcgtcg agcgtttgca gatagccgct ggcgcgcacc
    18541 atatattccg cttccgccag ttcgatcgac gaaccgcccg cttcctggtt tgaagcatcc
    18601 agcgcgcttt ttacttcggc gagactgatg ccatactgcg ccaggcgctg aggatcgata
    18661 accacctgat actctttcac cacaccgccc accgacgcca cttccgcaac gtcagggatg
    18721 gtttttagct catatttgag aaaccagtcc tgcaatgagc gtaaatcggc cagatcgtgc
    18781 ttaccgctgc gatccaccag tgcatattca tagatccagc caacacccgt ggcatctggc
    18841 cccagctcgg cgctgactcc cgcaggcagc ttaccctgta cctggttgag gtactccagc
    18901 acccgcgagc gcgcccagta cggatcggtg ccatcttcga aaatgacata cacataagag
    18961 tcgccaaact gcgagaaacc gcgcacagtc ttcgcgccag gcaccgacaa catggtggtg
    19021 gttagcggat aagtcacctg attttcaacg atttgcggtg cctgaccggg atagctggtt
    19081 ttaataatca cctgcacatc ggaaagatcc ggcagtgcat ccactggcgt gttaatgata
    19141 gtccaggtgc cccagatact cagaaacaac gcgcccatca gcaccagaaa acggttcgcc
    19201 accgagcgac gaataatcca ttcaatcatt ggctattccc tcaatgcgca tgggtagcac
    19261 tttcagagcg catccgctcc agtgcgccag aaatattggc ttcagaatca atcaggaaca
    19321 ggccgctgga aaccaccttt tcaccttccg ccagaccaga gcgtaacgcg gtgacgcctt
    19381 gcgatgcctg gaaaacggca acgcgtttcg gtacaaagcg cccgtcagca tcaacggtaa
    19441 tcacccgctg ttcgttaccg gtatcaatca gcgcctgtga cggaatgagc agcatcggtt
    19501 cgctggcggt attgagttgc aaccaggcgt tcatgcccgg ttttagcgcc tcgtcggcgt
    19561 tgtcgacttc cagacgcagt tgcagcgtgc gggtcgcagc atccacgcca ggtagcagcg
    19621 tccatttgcg gatggtgagt gttttatcag gtcgcgccgg aacggtgagc gtaaactgcg
    19681 aggcatcttt caccagccag gcgatggact ccgggatcgc agcagtgacc cacaccgggt
    19741 ccataccctg aattttcgcg accacgttat ctttggcgat attcattccc gcgcgcagat
    19801 caaacgcggt gatcacgcca tcaatgggcg ctttgagcgt aaagcgagtc tggatttttt
    19861 gcgtggcgat caggcggcga atatcagcct ccggcattcc cgccagtcgc agtcgctcaa
    19921 gaatgccttc agtctgggtc gccgtaccgc cggtttcgcg cagcagtaaa tactcactct
    19981 gcgcttccac ccagtcggga atggtcaggt cgagaagcgg cgcgcccttt tgcactttat
    20041 cgcccacggt aagcggatac accttgtcga taaacccggc ggcgcgggcc tgcacaatgg
    20101 catactgata ctcgttgtaa ctgacattcg ccgggaaact ctgggaaaaa gtcagcggcc
    20161 cgcgcgtgac ggtcgccgtt ttcaccccca gattctgcgt ctgagtcggg tcaatgcgca
    20221 caccagacgc agaactctct tcatcggcat atttcggcac cagatccata tccataaacg
    20281 gcgatttccc tggtttatcg aaccgcgtat tgggatacat cgggtcgtac cagaataaga
    20341 ctttacgttc tgcggtcgac gttttttctg cgggcggttc ctcctttgca aaccaggtaa
    20401 aacctgccgc agaaataata ccgcccgcga tcatgctgcc gataataagc gcgatttttt
    20461 tcatctcatt aaacccggat tactggctga ctttaatatc ctgtaataaa gaaaggttgc
    20521 cctgttggac aaaattaaac gctactttat cgccggtttt aatttcgctc attttcgtct
    20581 gcggggtgat ggtaaagcgc atagtcatct ccggccagtt cacggcagca atcggatcgt
    20641 gatggatggt gatttttttg ctttccagat caacatcttt taccacgcca gtggcgctaa
    20701 taacctgtgg ttgtgcttcg ctcatggttt catgatgatg ttcgttagcc tgggcattaa
    20761 agccaataac ggtaaacaga ctaaacattg cgacttgcag tgcttttttc atttattctc
    20821 tcctggagtt aaaaattatt gctgccaacc gccccctaag gcggtatata aagaaatttc
    20881 gttaacctga cgggcataat tcaggtcaag taaagtttgt cgggttgcaa ataaagaacg
    20941 ctcggcatcc agcacttcca gataacttac cgcgccgtgc tgatataatg cccgcgcccg
    21001 ttgcaaagta atttgcagcg acgcaagata acgctgctgg gcgctgattt gatcgttcag
    21061 gctttgacgt aatgccagcg catctgccac ttctttaaag gcgttctgaa ttttctgttc
    21121 ataattcacc accgactgct gctggcgaat ttcggcgata tccagattgg cctggttgcg
    21181 tccagcatta aaaatgggga tctcaatttt aggaataaaa ttccacatcc cgctgctggc
    21241 attaaataat gacgacagat cgctgctggc ggttgatatt ccgctggtca ggctgataga
    21301 cgggaaaaat gccgcacgcg cagcaccaat attggcatta gccgccatta acgcgtgttc
    21361 agcttccata atatcagggc gctgcaataa gatttgcgac ggcaagcccg ccggtaattt
    21421 aacgctttgc aggctgtcgc tgtttactgt ctgcgcttgc ggcagcttgc cgtagcttcc
    21481 caataacagt tgcaatgcat tatttgcctg cgccagttcc ccctgacgtt tagcgatgtc
    21541 gctgcgggta ctttctatca ccccgcgagc ctgttccagc gccagaacat tgctgctacc
    21601 ggtcaacagt tgtttttcga caaacgcata tgactgctga taattacgca gcgtttcttc
    21661 ggctatttgc aattgcgcat acgccagttg ctgattgaaa tagctttgcg cgacattaga
    21721 aaccaacaga atatgcaccg cgcgctgagc ttcctcagtg gctaaataat tttggcgctc
    21781 ggcttcgctc atgttcttta agcgaccgaa aaaatcgaga tcaaagctgg cgttaaggcc
    21841 agtcgataac tcccgcgtcg tggctgaatc gcctttaaga ttgccgctcc agctgccgct
    21901 gccctcgcca ttgagctgtg ggtagcggtc ggcatcggtc agacgatatt gcgcccgcgc
    21961 ttcctgcact ttcagcgccg ccatgcgcaa atcccggtta ttcaccaacg cctcgctaat
    22021 cagcgtcttc acctgattat caacaaaaaa ggtgcgccag cccgcgttct ggtagttatc
    22081 tgctgcgtta accaggccgt tctggctgag tgagaactgc tgcggcacgg gcattgccgg
    22141 acgctgataa tccggtgcca gtgaacaacc ggttagcgca agggccacac aaaatggcag
    22201 aagtttacaa ggagacatag gctcataatt tctggtgatt ttatgctgcc aactttactc
    22261 gccaggctct gattttccgg tgacaggaaa atgacaaaat tgtcattttg ccaataagcg
    22321 attgccatct gatcccgcta ctctagaatt gcccgggcaa catgcggagg aaatatgaaa
    22381 ctgttgattg tcgaagatga aaagaaaacc ggagaatact tgaccaaagg gttaaccgaa
    22441 gccggttttg tggtcgattt ggccgacaac gggctgaatg gctaccatct ggcgatgacc
    22501 ggtgattatg atctgataat cctcgatatt atgctgccgg acgtgaacgg ctgggatatc
    22561 gtgcgcatgt tgcgttccgc caataagggg atgtcgattc tgttgcttac cgcgcttggc
    22621 accattgaac atcgcgtcaa ggggctggag ttgggggcgg atgactatct ggtgaagccg
    22681 ttcgcttttg ctgaactgct ggcgcgggtg cgcactctac tgcggcgcgg agcggcggta
    22741 attatcgaaa gtcagtttca ggttgccgat ctgatggtcg atctcgtcag ccgcaaagtc
    22801 acccgcagcg gcacgcgcat cactctgacc agtaaagaat ttactctgct ggagtttttc
    22861 ctccgccatc agggcgaagt gctgccccgc tcgcttatcg cctcgcaggt atgggacatg
    22921 aattttgaca gcgataccaa cgccattgat gtggcggtga agcggctgcg cggcaaaatc
    22981 gacaacgact ttgagccaaa gttgattcag accgtgcgcg gcgtgggtta catgctagag
    23041 gtgccggatg gtcagtaagc catttcagcg cccgttttcg ctggcaaccc gcctgacctt
    23101 ttttatcagc ctcgccacca tcgcggcgtt tttcgccttt gcatggatca tgatccactc
    23161 ggtaaaagtg cattttgccg agcaggatat taatgattta aaggagatta gcgccaccct
    23221 tgaacgggta cttaatcacc ctgacgaaac gcaagcccga cgcttaatga cgctggaaga
    23281 tatcgtcagt ggttattcca atgtgttgat ctccctggca aatagccacg gtaaaacggt
    23341 gtatcactcc cccggtgcgc cggatatccg cgagtttacg cgtgacgcca tacccgataa
    23401 agacgcgcag gctggcgagg tgtatctcct ttccggcccg acgatgatga tgccagggca
    23461 cggtcacggg catatggaac acagcaactg gcggatgatt aacttgccgg ttggcccgct
    23521 ggttgacggc aaaccgattt atacgctcta catcgcgctt tcgatcgatt ttcatcttca
    23581 ttacatcaat gatttgatga ataaacttat tatgaccgca tcggtaatca gcatcctgat
    23641 cgtctttatc gtgctgttgg cggtacataa aggtcacgcg ccgatccgca gcgtcagccg
    23701 tcaaatccag aatatcacct cgaaagatct cgacgttcgc cttgacccgc agaccgtgcc
    23761 cattgagctg gaacagctgg tactgtcgtt caaccatatg atcgagcgta ttgaggatgt
    23821 ctttacccgc cagtccaatt tctcagcgga tatcgcccac gaaattcgca cacctattac
    23881 taatctcatt acgcaaacgg aaatcgccct cagccagtcg cgcagccaga aggagctgga
    23941 agatgtgctc tactctaatc tcgaagagct gacgcgaatg gcgaaaatgg tcagcgatat
    24001 gctgtttctc gctcaggccg ataacaacca gctaatcccc gaaaagaaaa tgctcaacct
    24061 gggggatgaa gtcggcaaag tgttcgattt tttcgaggcg ttagcagaag atcgcggcgt
    24121 ggagctgcgg tttgttggcg acgagtgtca ggttgcgggc gatccgctga tgctgcgtcg
    24181 ggcgttaagt aacctgcttt ctaatgccct gcgttatacg ccaaccggag agacaattgt
    24241 tgtgcgctgc cagacggtcg atcacctggt gcaagttacc gtcgaaaacc ccggtacgcc
    24301 cattgcgccc gaacacttac cgcgattgtt tgaccgtttc taccgcgttg atccttcccg
    24361 ccagcgaaaa ggcgaaggta gcggcattgg tctggcgata gtgaaatcga ttgttgtcgc
    24421 gcataaaggc actgttgcag taacgtcaga tgcacggggg acaaggtttg ttatcatatt
    24481 acccgcttaa tccttcagca aacggcaact tttataacca gtgtaaaaat aacatgccgc
    24541 aatagtaata cctattaatg ataaaaagct gtcacaattc ataaaaaacc ttaatatacg
    24601 ccaccctaaa cataaccagc gttaatgtaa ggtttttgtg tggactggct tcttgatgtt
    24661 tttgctacct ggctctacgg cttaaaagta atcgcgataa cgttagcggt cattatgttc
    24721 atcagcgggc tggacgattt ttttattgat gtcgtctact gggtacgccg cattaaacgc
    24781 aagttgagtg tttatcgccg ctacccgcga atgagttacc gcga
//
LOCUS       DAZFFE010000070        24519 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFFE010000070 DAZFFE010000000
VERSION     DAZFFE010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.070"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(134..1336)
                     /gene="ubiI"
                     /locus_tag="ACHJ69_003004"
                     /gene_synonym="visC"
     CDS             complement(134..1336)
                     /gene="ubiI"
                     /locus_tag="ACHJ69_003004"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO5015943.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSVINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYN
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEALRMQQASEDEFNRALNIAFDNRLGLCKVESERQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(1359..2537)
                     /gene="ubiH"
                     /locus_tag="ACHJ69_003005"
                     /gene_synonym="visB"
     CDS             complement(1359..2537)
                     /gene="ubiH"
                     /locus_tag="ACHJ69_003005"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO5015944.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALSVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATVCGVDWQQDPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAAKP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(2534..3859)
                     /gene="pepP"
                     /locus_tag="ACHJ69_003006"
     CDS             complement(2534..3859)
                     /gene="pepP"
                     /locus_tag="ACHJ69_003006"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO5015945.1"
                     /translation="MSDISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEHAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLENSLRLYRPGTSIQEVTGEVVRIMVSGLVKLGILKG
                     EVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(3885..4463)
                     /gene="ygfB"
                     /locus_tag="ACHJ69_003007"
     CDS             complement(3885..4463)
                     /gene="ygfB"
                     /locus_tag="ACHJ69_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO5015946.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLIHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            4631..4960
                     /gene="zapA"
                     /locus_tag="ACHJ69_003008"
     CDS             4631..4960
                     /gene="zapA"
                     /locus_tag="ACHJ69_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO5015947.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            5002..5185
                     /gene="ssrS"
                     /locus_tag="ACHJ69_003009"
     ncRNA           5002..5185
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHJ69_003009"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5206..5808
                     /gene="fau"
                     /locus_tag="ACHJ69_003010"
     CDS             5206..5808
                     /gene="fau"
                     /locus_tag="ACHJ69_003010"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO5015948.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(5910..5969)
                     /gene="ibsC"
                     /locus_tag="ACHJ69_003011"
     CDS             complement(5910..5969)
                     /gene="ibsC"
                     /locus_tag="ACHJ69_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO5015949.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            complement(6198..7430)
                     /gene="serA"
                     /locus_tag="ACHJ69_003012"
     CDS             complement(6198..7430)
                     /gene="serA"
                     /locus_tag="ACHJ69_003012"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO5015950.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(7686..8345)
                     /gene="rpiA"
                     /locus_tag="ACHJ69_003013"
     CDS             complement(7686..8345)
                     /gene="rpiA"
                     /locus_tag="ACHJ69_003013"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO5015951.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            complement(8401..8631)
                     /locus_tag="ACHJ69_003014"
     CDS             complement(8401..8631)
                     /locus_tag="ACHJ69_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5015952.1"
                     /translation="MHFAQQVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RPLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            8772..9665
                     /gene="argP"
                     /locus_tag="ACHJ69_003015"
     CDS             8772..9665
                     /gene="argP"
                     /locus_tag="ACHJ69_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO5015953.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            9868..10962
                     /locus_tag="ACHJ69_003016"
     CDS             9868..10962
                     /locus_tag="ACHJ69_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HFO5015954.1"
                     /translation="MSNVLVWQQLANNELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDFDNPRVAGDVGKAGVAIDTVEDMKELFDQIPLDKMSVSMTMNDAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKQSMRIIADIIAWCS
                     GNMPRFNNISISGYHMGEAGANCVQQVAFTLADRIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLGAARYLWSEAVSGFGAQDPYNNVIRTTIDHCAHPMYRDYLHRYL
                     ENASGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(10959..11855)
                     /gene="ygfI"
                     /locus_tag="ACHJ69_003017"
                     /pseudo
     CDS             complement(10959..11855)
                     /gene="ygfI"
                     /locus_tag="ACHJ69_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(12013..12753)
                     /gene="yggE"
                     /locus_tag="ACHJ69_003018"
     CDS             complement(12013..12753)
                     /gene="yggE"
                     /locus_tag="ACHJ69_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO5015955.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFNRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQPPAKTPAAH"
     gene            complement(12846..13481)
                     /gene="argO"
                     /locus_tag="ACHJ69_003019"
     CDS             complement(12846..13481)
                     /gene="argO"
                     /locus_tag="ACHJ69_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO5015956.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(13620..14480)
                     /gene="mscS"
                     /locus_tag="ACHJ69_003020"
     CDS             complement(13620..14480)
                     /gene="mscS"
                     /locus_tag="ACHJ69_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO5015957.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPARRNEFIIGVAYDSDIDQVKQILTDIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(14671..15750)
                     /gene="fbaA"
                     /locus_tag="ACHJ69_003021"
     CDS             complement(14671..15750)
                     /gene="fbaA"
                     /locus_tag="ACHJ69_003021"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO5015958.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKENEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(15965..17128)
                     /gene="pgk"
                     /locus_tag="ACHJ69_003022"
     CDS             complement(15965..17128)
                     /gene="pgk"
                     /locus_tag="ACHJ69_003022"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO5015959.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(17178..18197)
                     /gene="epd"
                     /locus_tag="ACHJ69_003023"
     CDS             complement(17178..18197)
                     /gene="epd"
                     /locus_tag="ACHJ69_003023"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO5015960.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            18569..19000
                     /locus_tag="ACHJ69_003024"
     CDS             18569..19000
                     /locus_tag="ACHJ69_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO5015961.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVLSQLRAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQENTALLSGKFEVIALNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGCLEELAFSRQLCALSGYDELHISPVK"
     gene            19023..19601
                     /locus_tag="ACHJ69_003025"
     CDS             19023..19601
                     /locus_tag="ACHJ69_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO5015962.1"
                     /translation="MARRHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            19602..20207
                     /locus_tag="ACHJ69_003026"
     CDS             19602..20207
                     /locus_tag="ACHJ69_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFO5015963.1"
                     /translation="MHPFTSLTLWALAACTTLILPTQTILPVYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWAHAITLWLRILAIVSTSQL
                     WMQYVPVQRFIRALFASRLPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRA
                     FRINNRRTTLWAPADSTLQRVARYTMILLMLAEFGAWIWLR"
     gene            20195..20872
                     /locus_tag="ACHJ69_003027"
     CDS             20195..20872
                     /locus_tag="ACHJ69_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5015964.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGRLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEKEIMARIDAALALTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPKASEMLLQRLQHWALERGSLIILFERHHTPFLNHCQQAWQLQN
                     GALQPLC"
     gene            20866..21543
                     /locus_tag="ACHJ69_003028"
     CDS             20866..21543
                     /locus_tag="ACHJ69_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5015965.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTADTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LILQKCPADEITQRTNAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PSDDIHP"
     gene            complement(21515..22228)
                     /locus_tag="ACHJ69_003029"
     CDS             complement(21515..22228)
                     /locus_tag="ACHJ69_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO5015966.1"
                     /translation="MKIELTVNGLKVQAQYSDDEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDVHQLRPFKGA
                     PETFNVAKLAENLCRVVEGDCTWPQYDRQKHDPVEDVLHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFIKAPASALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLMD"
     gene            complement(22225..22734)
                     /gene="fumE"
                     /locus_tag="ACHJ69_003030"
     CDS             complement(22225..22734)
                     /gene="fumE"
                     /locus_tag="ACHJ69_003030"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO5015967.1"
                     /translation="MATLTEDDVLEQLNAQDNLFSFMKIAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPRFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(22756..23071)
                     /locus_tag="ACHJ69_003031"
                     /pseudo
     CDS             complement(22756..23071)
                     /locus_tag="ACHJ69_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
     gene            complement(23068..24345)
                     /gene="yggP"
                     /locus_tag="ACHJ69_003032"
     CDS             complement(23068..24345)
                     /gene="yggP"
                     /locus_tag="ACHJ69_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO5015968.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVNVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPGG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QMLLPVELAASKGIELVYMNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LAWLVEKTHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(24360..>24519)
                     /locus_tag="ACHJ69_003033"
     CDS             complement(24360..>24519)
                     /locus_tag="ACHJ69_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5015969.1"
                     /translation="NVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLDTLFN
                     QLTAEHKH"
BASE COUNT         5965 a         6588 c         6020 g         5946 t
ORIGIN      
        1 atccataaca ttagaaaata taatgcgaaa acggaggtta aattagttta tttcaaatga
       61 ggaaaatctc ccggcgaaaa aaccgggaga tgaaaatgtg atgggtatca aataaacagc
      121 agaagagaaa tatttaacgc agccattcag gcaaatcgtt taatcccatt gcctgacgaa
      181 taagttgtgg cttaacgcca ggaagcgtat cagccagttt cagaccaata tcacgcagca
      241 attttttcgc cggattagcc ccggaaaaca gatcgcggaa tccctgcata ccagccagca
      301 tcagcgccgc gctgtgcttg cggctacgct catagcgacg caggtaaatg tactgcccga
      361 tgtctttacc ctgacgatgc aaccgtttca gttcagcaat cagctctgca gcatccataa
      421 agccgagatt taccccctgc cccgccagcg ggtgaatggt atgcgcggcg tcgcccacca
      481 acaccagacg gtgcgcggca aactggcgcg cataacgccc cgtcagcggg aacacctgac
      541 gctcgctctc aaccttgcat aagcccaggc gattatcaaa agcgatattt aacgcgcgat
      601 taaattcgtc ttcactagcc tgctgcatcc gcagcgcttc ctctggcgac agtgaccaga
      661 caatcgagca aagatgcgga tcgctaagcg gtaaaaaggc cagaatgcct tcgccatgga
      721 aaacctgccg cgccaccgca tcatgcggtt cttccgtgcg aatggtcgcc accagcgcgt
      781 gatgctgata atcccagaaa gtcagcggaa tatcggcttt gttgcgcaac caggaattag
      841 cgccgtccgc gccaatcacc agacgcgccg ttaacatgct gccatctttc aacgtcagga
      901 aggtttcatt ttctccccag gcgacctgct gtaattctgc gggggccaac agagtgatat
      961 ctgacgactg ctgcgctttg ttccacagcg cgtagtgaat cactgagttt tcaacgatat
     1021 gcccaagatg gttatagccc atgctttgat cgtcaaacga gatgtgacca aaactgtctt
     1081 tgtcccacac ttccataccg tgatagcaac tggccctacg agagagaatg tcctgccaga
     1141 cgccaagacg agtcagtaat ttttcgctgg cggcattgat aaccgaaacg cgcagttgtg
     1201 gtggtgcatc cgccgccaga ggttccggta cgcgctgctc cagtacggca acgcgtaagc
     1261 cgctaccctg taagccgcag gcaaccgcca gcccgaccat gccgccgcca acaatggcta
     1321 catcaacact ttgcattgtt tattccttaa aaccgccttc aacgcgccac ccaaccaaga
     1381 gtgcgctgcg ccagcacatc gcgtgccggg gtaaataatt ccatcgtcat cagcccgatg
     1441 ttgcgcccga caaccagcgg cgtccaacgg ttggcaaaaa gatgtacaag gctgtccgtg
     1501 acgccgatgg ttgcttcgcg atcgctctgt cgacgctgct gataacggca caatacgccg
     1561 taatcaccca tgtcttctcc gcgttcctgc gcctgagtca gggtttccgc aaggctcatg
     1621 acatcacgca taccgaggtt aaatccttgc ccggcgatgg ggtgcagagt ttgcgccgca
     1681 ttgcccacca gcacggtacg atgggtaata ggtttggcgg cgcgggttag cgctagcgga
     1741 taagcactgc gtttaccggc atgagtaatt ttcccaagtc gccagccaaa ggccgactgg
     1801 agttcacggc aaaacttctc gtcgctccac gacagcacct cttcgcgccg ttccagtgga
     1861 tgacaccaga ccagcgaaca gcgtccgtca gacatcggca acatcgccag cgggccatgt
     1921 tgcgtaaagc gttcaaaagc gcgcccttca tgcgcaacgg aagtcgcaac gttcgcaatc
     1981 acagccagtt gttcgtaagg atcctgctgc cagtcaacgc cgcacacggt ggctaacgct
     2041 gaatgggtgc catcagcggc taccagcacg cggcccgtca gcgtctcacc actctccagc
     2101 gtcacttcaa cgtggctctg agtacgggca acgttagcca cgcgatcagg gcaatgcagc
     2161 gttacgccag gtgctttgcg cagcaatgca aacagccgtt gcccgacatt gtgcaattcg
     2221 acaacctgcc ccagcgccgc cagttggtaa tcttctgcgg caagggtgac aaatccagcg
     2281 tgaccacgat cgctgacatg cactgtggtg atggcagttg cgcaatcagc cagagattgc
     2341 cagacgccga tgcgcgccag ttgctgacag gtacccgccg ccagcgcaat agctcgtcca
     2401 tcaaagcccg gatgcgcatg tgactctggc gcggtcgctt caatcaaatg tactgacagc
     2461 gccccgtgac ttaaccgtga aatagccagc gccagcgtcg cgcccgccat gccgccaccg
     2521 acgatgatta cactcattgc tttctcgcag ctgccatcaa cgcttcgatt tcttccggct
     2581 ttttcaccac gctggcggtg aggttttcat taccggtttc ggtaatcaca atatcgtctt
     2641 caatacgaat gccgataccg cgatactgtt ctggcacttc tgcatccggc gcaatataca
     2701 gccctggttc tacggtcagt accatacccg gttccagaat acgcgagcga tcctgaccat
     2761 aaacaccaac gtcatgaaca tccagcccta accagtggct aaggccatgc ataaagaaag
     2821 gacgatgggc gttctgagcg atcagttcat caacttcacc tttcaggatg ccgagtttta
     2881 ccagaccgct aaccatgatg cgcaccactt caccagtgac ttcctgaatg gaagttcccg
     2941 gacgatacag gcgcaggctg ttttcaagag actccagcac aatgtcatag atttcacgct
     3001 gagcctgagt gaatttgccg ttgaccggga aggtgcgggt aatgtcgcca gcgtaacctt
     3061 tgtattcaca acccgcgtca atcaacacca ggtcaccgtc acgcagttca cactcgtttt
     3121 cggtgtagtg caaaatgcag ccgttttcac cgctgccaac aatggtgtta taggaagggt
     3181 aacgcgcacc gtggcggtta aattcgtggt gaatttcgcc ttccagatgg tactcgaaca
     3241 tccccggacg gcatttttcc atcgcccggg tatgcgccag tgcggtgatt tcacccgcgc
     3301 ggcgaagtac ggcaatctct tctggtgatt tgaacaggcg catttcatga acaacaggac
     3361 gccagtcgat catcgttgcc ggtgcggtga gattttgccg cgaaccttta cgcagtttct
     3421 caagcgcgct gttcacgatt tcatcagcat atgcatgttc gccctgagcg tggtaaacca
     3481 catccagacc attaagtagt tgataaagtt gctgattgat ttcgctgaat gccagtgcgc
     3541 ggtcaacgcc cagtttttct ggcgcggcat cctggcctaa gcgacgacca aaccagatct
     3601 ctgccgtcag gtctcgaacg cggttaaaca aaacgctgtg gttatgagtg tcatcgcttt
     3661 taatcagcac cagcaccgct tccggttcgt taaagccggt gaagtaccag aagtcactgt
     3721 tctgacgata ggggtattcg ctgtcggcgc tacgtgttac ttctggtgca gcaaaaatca
     3781 gcgcggcgct gccgggttgc atttgctcta ccagggcctg acggcgacgc tgaaattctt
     3841 gccgggatat atcactcata acactctcct tacgtttttt gtttttagtg tagagtcggt
     3901 ttttgtactt ctggcgcggt cggttgcgga tgagtaaagg tgtcgtggca taacagcgcg
     3961 gcaacacgga cgtactcgat gatctcttca agcgacattt caagctcttc ctgatcttcg
     4021 tcttcgtcgt aacccaactg cgcgatgtta cgcagatcgt cgatggcttc accggtttcg
     4081 ccggtcactt tgtccagctt cggttgcgta acgccaagac caagcaggaa gtgattgacc
     4141 caaccagcca gcgcatcagc ccgatcgaaa acgctgacat catcgccatc aggcagataa
     4201 agctgaaaaa ggaagccgtc atcctgcagg gcatcgctgg tggcagagtg cattttgcgc
     4261 agtgcctgtg ccagctcatg accgaaagcc atgccttcgt tcgtcaggtc gtgaattagc
     4321 ggcagccatg agctgtcatc gttaccgcca catatcatcc cactgattaa accatgcatc
     4381 tcagcagggg tcagacccgt cccttgttgg ttcagatact ggttcatttc gttgtaacca
     4441 ggcatttcgt tctgtataga cataagcatt cgtcgtcaaa gggaggaata ttcatgatat
     4501 gctaccactt tgggccctgg tggaccagaa aagggcttgt ctcttctcat cagggtagct
     4561 atagtgtcgc cccttcgcag accatgggtc taaagacgaa ggcagcgcag tcaatcagca
     4621 ggaaggtggc atgtctgcac aacccgtcga tatccaaatt tttggccgtt cactgcgtgt
     4681 gaactgcccg cctgaccaaa gggatgcgtt gaatcaggca gcggacgatc tgaaccaacg
     4741 gttgcaagat ctgaaagaac gcactagagt cacaaatact gaacagttgg tcttcattgc
     4801 cgcattgaat atcagctatg agttagcgca agaaaaagcg aagactcgtg actacgcggc
     4861 aagtatggaa cagcgtattc ggatgctgca gcagaccata gaacaagcgt tacttgaaca
     4921 aggtcgcatc accgaaaaaa ctaaccaaaa ctttgaatga cacttttcgg tttactgtgg
     4981 tagagtaacc gtgaagacaa aatttctctg agatgttcgc aagcgggcca gtcccctgag
     5041 ccgatatttc ataccacaag aatgtggcgc tccgcggttg gtgagcatgc tcggtccgtc
     5101 cgagaagcct taaaactgcg acgacacatt caccttgaac caagggttca agggttacag
     5161 cctgcggcgg catctcggag attcccttct tatctggcac cagccatgac gcaactacca
     5221 gaacttccac tgacattatc ccgacaagaa atccgcaaaa tgattcggca acgtcgtcgt
     5281 gcgttaacgc cggaacaaca gcaggaaatg ggtcaacaag ccgctacccg gatgatgact
     5341 tatcccccgg tggtgatggc acatacggtc gctgtattcc tctcttttga tggcgaactc
     5401 gacactcagc cactcataga acaactctgg cgcgccggta agcgcgtata tcttccagtt
     5461 ttgcatccct ttagtgccgg taatttgctg ttcctgaatt accatccgca aagcgaactg
     5521 gtgatgaaca ggttgaagat ccatgagcca aaactggatg tgcgtgacgt gctacccctt
     5581 tcccgattag acgtgttgat cacaccgctg gtcgcctttg atgagtacgg tcagcgcctg
     5641 ggaatgggcg gtggttttta tgatcggacc ttacaaaact ggcagcacta taaaatgcaa
     5701 ccggtgggtt atgcgcatga ttgtcagttg gtggaaaaac tccccgttga agagtgggat
     5761 atccctcttc ctgcggtggt tacaccgtcg aaagtctggg agtggtgagg gcgacacatc
     5821 ccgcatcgcc ctgattgaca tcgttgattc tttaacctaa tttagtgagt aagggtaagg
     5881 gaggatgact cctcccctga gactgactgt taataagctg ggaaacttat gagtaacagt
     5941 acaatcagta tgatgacaag tcgcatcata acccttctcc ttcaagccct cgcttcggtg
     6001 agggctttac cgttacagcc ccatgctgcc ctgccatcgt aaatccccat taaataaaca
     6061 caacgcattg atctgacttt gatttatttt ctggagcaga ctcgcaaagt agaatgcgca
     6121 atgcggcaac ggtgtggaga agggataaaa aaacgggcaa gtcagtgacc tgcccgttga
     6181 ttttcagaga aggggattta gtacagcaga cgggcgcgaa tggtacccgg aatagctttc
     6241 attgcctgca gcgctttttc ggcaacgtct tcgtcggctt caatatcaat aactacataa
     6301 cccatctggg cggaagtttg tagatattgc gcggcgatgt tgacgccctg ctcggcaaaa
     6361 attttgttga gcgcagttag cacgcccgga cggttttcgt ggatgtgcat cagacgacgc
     6421 ccaccgtgca gtggcagcga gacttccggg aagttcaccg cagagagcgt tgagccattg
     6481 tcagaatact tgatcaattt acccgcaact tccaggccga tattctcctg cgcttcctga
     6541 gtcgaaccgc caatgtgtgg cgtcagaagg acgttgtcaa attcacacag cggagaggta
     6601 aatggatcgc tattggtcgc cggttcagtc gggaatacgt cgattgccgc ccccgccaga
     6661 tgtttgctcg ccagcgcgtc acacagcgct ggaatatcca ccacagtacc gcgcgaagca
     6721 ttaatcagca gcgagccggg tttcattagc gaaatctctt tcgcgcccat catatttttg
     6781 gtggacggat tctctggtac atgcagactc accacatcgc tcatattcag caggtcagaa
     6841 agatgctgta cctgagtggc gttgcccagc ggcagtttgt tttcaatatc ataaaagtaa
     6901 acatacattc ccagcgattc agccagaatg cccaattgcg taccaatatg accgtagccg
     6961 atgataccca gctttttgcc gcgcgcttca aaagaacccg ccgccagttt gttccacacg
     7021 ccacgatgcg ctttagcatt ggcttccggc acgccgcgca atagcagcag cagttcgcca
     7081 atcaccagct ccgcaacaga gcgcgtattt gagaacggtg cgttaaatac cgggatcccg
     7141 cgctttgccg ccgcatccag atcaacctgg tttgttccga tacagaaaca gccaatagcg
     7201 accagttttt ctgcggcgtt gatcacgtct tcagtcagat gggtacggga tcgcaggccg
     7261 atgaagtggg catcgcggat ggattctttt aattgttcat catccagcgc gcctttgtga
     7321 aattcgatgt tggtgtaacc agctgcacga aggctttcca gcgccttttg gtgcacgcct
     7381 tctaccagca gaaacttaat cttgtctttc tccagcgata cctttgccat ttacccaatc
     7441 ctgtcttttg aaatgttgtg tgcggatttg catccgcctt tcaacatatc aaaaaataat
     7501 attgcggcaa tatgaacgtt tgcgtcgcga tgttgaggaa atatcacgca aaagtatttc
     7561 ttggatgaat atgctggttt agaagattta tttagagcaa tcgacaattg ccaggtaaaa
     7621 acgtgacaca tgtcaccaaa tttaatgaag agaatttttt taacggggga ggttcccccg
     7681 tcagatcatt tcacaatagt tttgacaccg tcaggtgtgc caatcagcgc aacgtccgcg
     7741 ccacggttag caaacaaacc aacagtcacc acaccaggaa tcgcatttat ggcgttttcc
     7801 atcgctatcg ggtcaaggat ttccatgccg tggacgtcga ggatcacgtt gccgttatcg
     7861 gtcactacgc cctgacggta ttccggacga ccgcccagtt tcaccagctg gcgcgccact
     7921 gcactacgtg ccatcgggat aacttctact ggcagcggga atttgcccag aatatcaacc
     7981 tgcttggaag cgtctgcaat acagataaat ttttccgcaa ccgaagcaat gattttttca
     8041 cgggtcagcg ccgcgccgcc gcctttgatc atttgcatgt ggccgttgat ttcatctgcg
     8101 ccatcaacgt agataccaag gctgtcgact tcgttgagat caaaaacgtg aatgccgagg
     8161 cttttcagtt tttcagtgga agcatctgaa ctggaaacgg ccccttcaat ctggcctttc
     8221 attgtaccga gtgcgtcaat aaagtgtgcg gcggtggaac ctgtacctac accaacaatg
     8281 gtgccgggct gaacatactg aagtgccgcc catcctactg cttttttcaa ttcatcctgc
     8341 gtcatgatcg tttcgcctgt ggtatgaaat ttcacacgca ttatatacaa aaaaagcgat
     8401 tcagaccccg ttggcaagcc gcgtggttaa ctcatccata aaatatcgcg caatggcggg
     8461 catccccttt cgccccgcaa ataaagcata caacggtctg ggtatgccgc tccacggtgc
     8521 aaacaggcgc accagttccc cgttcgcaag cccctgttta caggcaaact gaggcaataa
     8581 cgccacgcca ttcaacacaa ccagggcgcg aacttgctga gcgaaatgca ttagcgcaaa
     8641 tccctgatct tttaagctgc gcgttaacca ctgatacaaa tgttacccgc caacggcttc
     8701 ggtatatgca acccgacaca aaattgtgtc atagtgcagg aaaaagcatt taccaggagc
     8761 agacaacagc aatgaaacgc ccggactaca gaacattaca ggcactggat gcggtgatac
     8821 gtgaacgagg atttgagcgc gcggcacaaa agctgtgcat tacacaatca gccgtctcac
     8881 agcgcattaa gcaactggaa aatatgttcg ggcagccgct gttagtgcgt accgtaccgc
     8941 cgcgcccgac ggaacaaggg caaaaactgt tggcgctact acgccaggtg gagctgctgg
     9001 aagaagagtg gctgggcgat gaacaaaccg gttcgactcc gctgctgctt tcactggcgg
     9061 tcaacgccga cagtctggcg acgtggttgc ttccggcact ggctcctgtg ttggctgatt
     9121 cgcccatccg cctcaacttg caggtagaag atgaaacccg cactcaggaa cgtctgcgtc
     9181 gcggcgaagt ggtcggcgca gtgagtattc aacatcaggc gctgccgagt tgtcttgtcg
     9241 ataaactcgg tgcgctcgac tatctgttcg tcagctcaaa accgtttgcc gaaaaatatt
     9301 tccctaacgg cgtaacgcgt tcggcattac tgaaagcgcc agtggtcgcg tttgaccatc
     9361 ttgacgatat gcaccaggcc tttttgcagc aaaatttcga tctgcctcct ggcagcgtac
     9421 cctgccatat cgttaattct tcggaagcgt tcgtgcaact tgctcgtcag ggcaccacct
     9481 gctgtatgat cccgcacctg caaatcgaga aagagctggc cagcggcgaa ctgattgact
     9541 taacgccagg gctattccaa cgccggatgc tctactggca ccgctttgct cctgaaagcc
     9601 gcatgatgcg taaagtcact gatgcgttgc tcgattatgg tcacaaagtt cttcgtcagg
     9661 attaatccat caaataatgc ctgatagcac atatcaggcg ttatcctcac ttctttttgt
     9721 attccttgaa tcacattaca aaatagacaa atctcaggcg gcaaaaaacg acgtctgaat
     9781 gcattttttt gctggcgaca aacccacgta aaaagctcac cgtaagcgca aataccctca
     9841 ctttgattgc gttttacgga gcaaataatg tctaacgtgc tggtgtggca acagcttgcc
     9901 aacaatgaat tgagccgtcg ggagaaaact gtcgactcgc tggttcagca aaccgcggaa
     9961 gggatcgcca tcaagccgct gtataccgaa gccgatctcg ataatctgga ggtgacgggt
    10021 acccttcctg gtttgccacc ctacgttcgt ggcccgcgtg ccactatgta taccgcccaa
    10081 ccgtggacca tccgtcagta tgctggtttc tcgacagcaa aagagtccaa cgctttttat
    10141 cgccgtaacc tggccgccgg gcaaaaaggt ctttccgttg cgtttgacct tgccacccac
    10201 cgaggctacg acttcgataa cccacgcgtg gcgggcgacg tcggcaaagc gggcgtcgct
    10261 atcgacaccg tggaagatat gaaagagctg ttcgaccaga tcccgctgga taaaatgtcg
    10321 gtttcgatga ccatgaatga cgcagtgcta ccagtactgg cgttttatat cgtcgccgca
    10381 gaagagcaag gtgttacacc cgataaactg accggcacca ttcagaacga tatcctcaaa
    10441 gagtatcttt gccgcaacac ctatatttac ccaccaaaac agtcaatgcg tattatcgca
    10501 gacatcatcg cctggtgttc cggcaacatg ccgcgattta ataacatcag tatcagcggt
    10561 taccacatgg gtgaagcggg tgccaactgc gtgcagcagg tagcatttac gctcgccgat
    10621 aggattgagt acatcaaagc agcaatctcc gccgggctga aaattgatga cttcgcccct
    10681 cgcctgtcgt ttttcttcgg cattggcatg gatctgttta tgaacgtcgc catgttgggt
    10741 gcggcacgtt atttatggag cgaagcggtc agtggatttg gcgcacagga tccgtataac
    10801 aacgttatcc gcaccaccat tgatcattgt gcacatccca tgtatcggga ttatctgcat
    10861 cgatatctgg aaaatgcgtc cggcggacat attcaccacg atcttagcca cgtcttcgac
    10921 ttacaccgta atttaattgc aaccggatcg atgctgggtt aatggtttgt cccgtcacga
    10981 tcttcagcaa tatgctgaag catcgtggcg gtataacgat gatttctgat tgaataaaga
    11041 tattcctgca tatacatcgg cctgtgcggc gcatcaaaat atttcagcgt tgccggattc
    11101 accagtcgcc aggcaaaatg cgggatcacc gtcagaaaat gtccccgctc cacggcgctg
    11161 attttagcca taaaactgta gggacgataa ataatggtgg gattgatccc acaaggacgc
    11221 atattcgcat caatcattgc ttcgaaattg gcccgattct gaaagcgcat ttgtagccag
    11281 ggcagctcct gaagcagttc ttgctcttgt cgctcttcat aacggcgaga aacgagaaag
    11341 cccaatcgca gcggcggtag ttcactgata gtcaggtttt ccagttcctg aactcgtgca
    11401 gatacatgct gtggggagat aataaaatct aactgacggt caaaaagatt atcgataacg
    11461 ccattttcgc tgaactcaac tggctgggcg gtcacgcctt cgtacttatc tccgagacta
    11521 atcaactgat caaaaataat tgtcggataa gtattatcta tgcctatcac aatttctcgt
    11581 taacgacgtc ctgagttatg gatttcatta tcaatcgcag aaagtcgttg ataaaccggg
    11641 aacagttttt gatacagttc ctgtccggct ttattgaggc tgatattatt atctttgcgg
    11701 gtaaatagcg tatagccaat ttgttcttcc agcgcggcaa tgcttttgcc aaatcgcgaa
    11761 gccgtcatat ggattttttc tgccgcccgg gcaatattat tggtttgcgc taataaaatg
    11821 aaattgcgca tctttttcga aataaaaatg tccatctccc ctctcccgca tccttgtccg
    11881 cttaaccgtt tcagtcagcc tatccttgat gaaaccgcga gcaaagatag gtgattacgt
    11941 catggtttta cagaaaaagg aggcaatatc gggtaaaggc attagcccga cgaatacgtc
    12001 gggctacaag tattaatgtg cagcaggtgt tttagcgggt ggttgatcca caggttctaa
    12061 ctggaagact acatcgacct gatcatcaaa ctgaatagcg gcctgctcgt aagtttcctg
    12121 tgcggacacc ggtgcagcat cagctttcat catccgcacc attgggctgg gctgatagtt
    12181 ggaaacatga taacgcacgc tatataccgg cccgagtttg cgattaaagc cattcgccag
    12241 ttcctgcgcc tgatgaatcg cgttatcaat cgctgcctta cgcgctttgt ctttataggc
    12301 atccggctgc gccacgccca gcgacacaga acgaatttcg ttaagacccg cctttagcgc
    12361 gccgtccagc aaggaattca gtttgtctaa ctgacggagc gtgacttcca ccgttctcac
    12421 tgcgcggtag cctttcagga tacttttacc atcctgataa tcataatctg gctgagtgcg
    12481 taagttcgct gagctgatat cttttttcgc gatctgattg agctcaagga aggaaatgta
    12541 ttgtgcgacg cgctcatctg cctgtttctt ggcagtagcg gcatccttcg cggccacgtt
    12601 aacttcaatc gcaagagtgg caatgtctgg caccgcatcc acgcttgccg taccggaggt
    12661 gacaatatgc ggtccatccg gcaattcgtt agcctgcgct gccatcccgc taatacccat
    12721 taatgccgcc agggcgataa ctttgaactt cacgttagtc tctccgtgtt aattgacttg
    12781 cccaaaattc aggacctctg gaggcaagct tagcgcctct gttttatttt tccatcagat
    12841 agcgcctaac tgaacaaggc ttgtgcatga gcaataccgt ctctcgccag ctgcaaggca
    12901 ataaaccaca taacacatcc cactaccaga ttgataatgc gctgtgattt tgccgtgcgc
    12961 aggcgcggtg ccagccaggc tgcgagaata gccagaccaa agaaccacag gaaagaggcg
    13021 ctaattgttc cgagcgcaaa ccaacgtttt ggttccacat caagttgccc gccaaggctg
    13081 cccagcacaa caaaagtatc caggtaaaca tgcggattca gccaggtcac tgccaacatg
    13141 gtggcgataa ttttccatct gccttgtttc agaacttcgg cactggctaa ctcaatgtta
    13201 ctgctcattg ctgttttaaa agcgccaaaa ccataccaca gcaagaaggc tacgccgccc
    13261 caggtgacca gcgccagcaa ccacggcgac tgcatcaata acgcgctgcc accaaaaatc
    13321 cccgcacaaa tcaggaccaa atcgctgata gcacaaagta aggcaatcat aatgtggtac
    13381 tgacgacgaa tgccctgatt catcacaaaa gcattttgtg gaccgagcgg taggatcata
    13441 gccgccccaa gtgcaagacc ttgaaagtaa taagaaaaca cgttggatat tccgaattaa
    13501 gtatctgatg cagagactat atcgcgggat tgttattagc ggaaattgat aatattaatt
    13561 ggcaatcaga aaaactaata agagagaggc cagagagtat tatctggcct cagcgttgat
    13621 tacgcagctt tgtcttcttt cacccgctta aagttcacat ccatttgcgg gtacgggaag
    13681 ctgataccgg cggcatcaaa ttcacgttta atacgctcca gcacatccca gtacacgttt
    13741 tgcagatcgc cgctgttgct ccagacgcgg accacgaaat taatcgacga tgcgccaagt
    13801 tcgttcagac gcacagtcat ttcgcgatct ttcaaaatgc gatcttcaga ctggataata
    13861 tcggtcagga tctgcttaac ctgatcgata tcggaatcat acgccacgcc aataataaat
    13921 tcgttacggc gagctggctc gcgggagaag ttaataatat tcccggcaat aattttaccg
    13981 ttcggaataa cgataatttt accgtctgca gtacgcatgg tggtggagaa aatctgcaca
    14041 ctcagcacag taccggctac gccgccgagg tcaacatatt ctccggcacg gaacgggcgg
    14101 aacatgacaa gtaacacgcc agcggccagg ttagacagtg acccctgcaa agccagacca
    14161 acagctaagc ctgcggcacc gagtacagca atgactgacg ctgtttgtac gcccacgcgc
    14221 cccagtgcag cgattagcgt aaaggcgata attccgtaac ggactaatgc agaaagaaaa
    14281 tcagcaacag tggcatcgat tttacgggag atcatcaggc gattcaccgc gttggaaatc
    14341 atccgcgcga taatcaaacc aacgatgatg atcgcgagtg ccgccacgat gtttactgca
    14401 taacttagca gcagcgcctg gttagccacc agccagcttc ccgcgccgtt tatgctatcg
    14461 acaacattca aatcttccat tcaatattcc ttttcaactg actcaaaatg gagaaataca
    14521 ctccgccgcc ttatgacgga cagtctgaca agggtaaaca ataaagcata attttgccaa
    14581 atagatcaca gatttctcat ctgtatcacc ttgcttttgg cgaaaaaaag acccgcagag
    14641 cgggccttga gataagcaga aaggaatatc ttacagaacg tcgatcgcgt tcagttcctg
    14701 gaatgctttt tccagacgag cgatcatcga agtctgaccg gcacgcagcc atacgcgcgg
    14761 atcgtagtat ttcttgttcg gctgatcttc gcctttcggg ttgcccagct ggccttgcag
    14821 gtaagcttcg ttttctttgt agtagttcag aacaccttcc caggttgccc attgggtatc
    14881 ggtatcgatg ttcattttca ctacgccgta gcttacggag tctttgattt cctgagcagt
    14941 agaaccggaa ccaccgtgga atacgaagtt caggctgttg tgcggcaggt tgtgtttctt
    15001 agaaacatat tcctgagagt cgcgcaggat ggtcggagtc agaacaacgt tacccggctt
    15061 gtaaacaccg tgtacgttac cgaaggacgc agcgatggtg aaacgcgggc tgattttgct
    15121 cagctcggtg tatgcgtaat caacgtcttc cggctgggtg tacagtgcag aagcgtccat
    15181 gtggctgttg tccacgccgt cttcttcacc accagtgcaa cccagttcga tttccagagt
    15241 catgccgatt ttggacatgc gctccaggta tttagagcag atttcgatgt tctcttgcag
    15301 agattcttca gacaggtcga tcatgtgaga agagaacagc ggcttaccgg tagcagcgaa
    15361 gtgtttttca cccgcgtcca gcagaccgtc gatccacggc agcagtttct tcgcgcagtg
    15421 gtcagtgtgc aggataaccg gaacaccata atgttcagcc atctgatgaa cgtgatgcgc
    15481 accagagatc gcacccagga tagcagcgcc ctgcggaacg tcagatttca cgcctttacc
    15541 agcgataaag gaagcaccac cgttggagaa ctgaacgata accggcgctt taactttagc
    15601 agcggtttcc agtacggcgt tgatggagtc agtaccgacg cagtttactg ctggcagtgc
    15661 gaagttgttt tcttttgcta cctggaaaac tttctgtacg tcatcaccag tgattacgcc
    15721 aggttttacg aaatcaaaaa tcttagacat gtctcttgtc ctgtatcgtc gggccttata
    15781 ctcgtcatac ttcaagttgc atgtgctgcg tctgcgttcg ctcaccccaa tcacttactt
    15841 atgtaagctc ctggggattc actctcttgt cgccttcctg caactcgaat tatttagagt
    15901 atgaaaaagt gcgtgctcta aaagcgcgct gaaacaaggg caggtttccc tgccctgtga
    15961 ttttttactt cttagcgcgc tcttcgagca tcgctactgc aggcagtact ttaccttcta
    16021 cgaattcgag gaatgcgccg ccgccagtgg agatgtagga gattttgtca gcaatgccga
    16081 acaggtcgat tgctgccaga gtgtcgccgc cgccagcgat ggagaacgct tcgctgtctg
    16141 cgatagcgtt agccacgatt tcagtacctt tgcggaagtt cgggaattcg aacacgccaa
    16201 ccggaccgtt ccacagaatg gttttcgcat tcttcaggat ttcagccagt tcctgagcgg
    16261 aagcatcacc gatatccagg atctgctcgt cagctttcac atcgttaaca gatttcaggg
    16321 tagccggtgc agtttcagag aactcggttg ctacgcgaac atcagacgga accgggatgt
    16381 tgcaggtggt cagcagacgt ttagcttcgt caaccaggtc agcttcgtac agggatttac
    16441 ccacatcgtg gccttgtgcc gcgataaagg tgttagcgat accaccgcca acgatcagct
    16501 ggtcagcgat tttagacagg gagtccagaa cggtcagttt ggtagatact ttagaaccac
    16561 caacgatagc caccatcggg cgagcaggtt ctttcagtgc tttacccagc gcgtccagtt
    16621 cagctgccag cagcgggcct gcgcacgcaa catcagcgaa tttaccgata ccgtgagtag
    16681 aagcctgcgc gcggtgagca gtaccgaatg cgtccattac gaacacgtca cacagtgcag
    16741 cgtatttttt ggacagggtt tcgtcgtctt tcttctcgcc tttgttgaag cgaacgtttt
    16801 ccagaacaac cagttcacct tcagcaacgt caacgccgtc gaggtaatct ttaaccagac
    16861 gaaccgggtt agacagtttg tctttcaggt agttaacaac cggcagcaga gagaattctt
    16921 cgttgtactc gccttcggta ggacgaccca ggtgggaagt taccatcact tttgcgcctt
    16981 gtttcagggc cagttcgatg gtcggcagag aagcacggat acgcgcgtcg ctggttactt
    17041 tcccatcttt tactggtacg ttcagatccg cacggataaa tacacgtttc ccagcaagat
    17101 ccagatcggt catcttaatt acagacatgg tgaatcctct cgttgattct aaaagttttg
    17161 cagacgctgc ttgcgtctta cctgaaagca acagtagcca tagctaacgt cgtgtcgagc
    17221 attcggttag caaagcccca ttcgttatcg caccagacca acgttttgat caggtgtgcg
    17281 ccactgaccc gggtttgagt gccatcgaca atggcactgt gcggatcgtg gttaaaatct
    17341 acagagacca acggcaattc cgtatagtca actataccat gaaatgcacc ttgtgctgct
    17401 ttttgcagca acaggttgac ttcattggct tttacaggtt tcttcaccgt cacgcttaaa
    17461 tcgattgccg tcacatttat ggttggcaca cgtaccgcaa tcgcctcaaa gcgatcgtta
    17521 aattgcggaa aaaatcgtgt gataccggcg gccagtttag tatcgaccgg aatgatcgac
    17581 tgactggctg cccgagtgcg acgcaggtca ggatggtatg catcaataac ctgttgatcg
    17641 tgcatggcgg agtgaattgt ggtcacagtg ccggactcaa taccgtacgc atcatctaac
    17701 aatttgatga cgggaattat gcaattcgtg gtacaggaag cgttagaaac gatgcggtgt
    17761 tccgcacgaa gttgatcctg attgacgcca taaacaacgg tcgcgtcgag atcgttactg
    17821 ccaggatgtg aaaagagcac ttttttagcc ccggcggcaa tgtgtgcttc accatgctcg
    17881 cgggagccat atacgccggt gcagtcgagg actacatcaa cgccaagttc acgccagggg
    17941 agcgattgca gtgaacgttc atgcaataca cggatggcgt catcaccaac aaaaagttga
    18001 tcgcgttcct gtcgtacttc ccatgcaaaa cggccatggc tggtgtcata tttcaacaaa
    18061 tgcgccatac ccgcagcatc cgccagttcg ttgattgcca ccacggtaat ttccgcacgg
    18121 cgtccggatt catacaaagc acgaaccaca ttacgcccga tgcgaccgaa gccatttatc
    18181 gctacgcgta cggtcataga tctcctgcaa ggttttccct gagcaaattt gccagacaga
    18241 gtaatccagc aaatcgttcg gggaaacctt acctgtcgca aactgcgact gattggttaa
    18301 ttgtcgaaca tttaatcgac tgaaacgctt cagctaggat aagcgaaacg tggaataaaa
    18361 ggaatgattg tccagtcaaa gaagacaatt atctgactta cgtcacgttt tcagccccgc
    18421 ccctgtggaa tttattccac tttgcagaat cacgaataag cgtagcaaaa tcttttcgtt
    18481 gtgccgtcac aaaccctgac ttatatcaga atatgactga tgcagcgtcg atattatccg
    18541 caatacgtcc gagtttcgga gtactccaat gatgacattg ccctacccat attcttcctc
    18601 cgcacgtttt tacgcgctgc gcctgctccc cgggcaggaa gttctctccc aattacgcgc
    18661 cttcgcgcag caacaacaac tccacgccgc gtggattgca ggttgtaccg gcagtctgac
    18721 tgacgtcgcc ttacgctatg ccgggcaaga aaacactgcc ctgctaagcg gtaagttcga
    18781 agtcatcgca ttgaacggca cgctggaaca gagtggtgaa catcttcatc tctgcgtttc
    18841 cgatccacac ggcacgatgc ttggcggtca tatgatgccc ggctgtaccg tgcggaccac
    18901 gcttgaactg gtgattggct gcctggagga actggcgttt agccgccagc tttgcgcgct
    18961 ttctggttac gacgagttgc atatttcccc cgttaaataa ctattttcag agaggttttc
    19021 ttatggcgcg tcgccacttt tccagtcagg cactggtgtt gatcgttatc tctatcgcca
    19081 tcaatatgat cggcggacaa ctggccagca tggttaaatt acctattttt ctcgactcca
    19141 tcggcacact tattagcgcg gtactgcttg gtccggtgat cgggatgctg accggtttac
    19201 tgaccaattt actctgggga ttgctgaccg acccgatcgc cgccgcattt gcgcccgtcg
    19261 cgatggtcat cggcctggtc gccggttggc tggcgcgagc gggttggttt cgcaccctgc
    19321 caaaagtcgt tgttagcggc gtgattatta cgcttgcggt aacggtcgtc gctgtaccgc
    19381 tgcgtactgc gctgttcggc ggcgtgacag gaagcggagc cgatttgttc gttgcctgga
    19441 tgcactcgat ggggcaaaac ctggtggagt cggtggcgat tacggtgata ggcgcgaacc
    19501 tggtcgataa aattctcaca gcggtgattg tctggctttt actgcgtcag ctgccgattc
    19561 gcaccacgcg ccattttccg gcaatggctg ccgtgcgcta aatgcatccg tttacatcct
    19621 taacgctgtg ggcgctggcg gcctgcacca cgcttatcct gcccacacag acaatattgc
    19681 cagtctatag cgcggcgacc tttttctgcc ttattgcgct aaaggccacg cggcggcggg
    19741 caaaatatgt cgtctggctg atgttctcgc tgggggctgg attgtggctg gtgcacggtg
    19801 gctggctgac agaatggcta agcggcacgc cacgctctcc agaacggtgg gcgcatgcta
    19861 ttacgctctg gttgcggatt ctggcgattg tttcaacctc acagttgtgg atgcagtacg
    19921 tccccgtgca acgttttatc cgcgctttgt ttgcttctcg cctgccgctg gatgagggct
    19981 ggtatcagcg tttacgtgcg atgcccgcgt taattattcc gctaacacac aatgcgctaa
    20041 acgatcttgc tgtccgtggt gcggcgctgg atatgcgggc atttcgaatc aacaataggc
    20101 gcactacgtt atgggcacca gccgatagca ccctacaacg cgtagcacgt tacaccatga
    20161 tattgctaat gctggctgaa ttcggagcgt ggatatggtt acgttagaac agtttcgcta
    20221 ttgcccgaca cactcaacgc atcctccgtt ctgttatgac tttcactatg tcaaaccggg
    20281 gatggtcgcc atctttggtg acaacggcag tggcaaaagt acgctggcgc agctgatggc
    20341 gggctggtat ccggactttc taccaggaga gataaccggc acggggacgc ttctcggtac
    20401 gcccattggg cgtctcccgc ttaatgaaca atccgccacc atccagctcg tccagcaatc
    20461 gccctatttg cagctctccg gctgtacctt tagcgtggaa gaagaagttg cctttgggcc
    20521 agaaaatctg tgtcttgctg aaaaagagat tatggcgcgg attgacgccg ccctggccct
    20581 gaccgagtgc cagccgttgc gccatcgtca cccggcaaca ctctccggtg gagagacaca
    20641 gcgtgtggtc atcgcctgcg ccatcgcgat gcagccaaag ctcttaattc ttgatgaagc
    20701 ctttagccgc ctgacgccga aggccagcga aatgctgttg cagcgcttac aacactgggc
    20761 gcttgaacgt ggttcgctga ttattctttt tgaacgtcat cacacacctt tcctgaacca
    20821 ctgccagcaa gcgtggcaat tgcaaaacgg agcgctccag ccactatgtt aacgttaaat
    20881 cagatatctt atcgctggcc tggcgcggca acggattgtc tttgcgacat ttctctgcaa
    20941 ctcaaacaag gtgaatggct ggcgctgacc ggcgacaatg gcgcgggaaa atcgacgctg
    21001 ttacgagtaa tggcaggtct tcttacgccc acagccgata cggttatgct gcaacaacaa
    21061 gcgatgaaaa acctgaaaaa ccgccagcgc gcggcaaaga ttggcgtact gtttcaggaa
    21121 gcggaaaatc agctttttca cagcaccgtt gctgacgaaa tcgcatttgg tttgatactg
    21181 caaaaatgcc cggcggatga aatcactcaa cgtaccaatg ctgccctgca atgctgtcag
    21241 ttagcagaca ctgccagcgc gcatccgtta gatctgcata gtgcgcagcg acggatggtc
    21301 gccgtcgcct gcctggaagc actctctccg ccactattgc tactggacga gccgagtcgg
    21361 gattttgacg aaaattggtt gagcgtgttt gaaagctggc tggaaaagtg ccgccagcga
    21421 ggcacgagcg tcgtagcaat cagtcatgac gccgcattta cccggcgtca tttctctcgt
    21481 gtggtgcgac tggaagatgg tgtaatcagg aacgttaatc catcagacga tattcacccg
    21541 tagcggtcat cattaaggtc aaatttgcag gcagactctc ttccagcacc cggcgaacat
    21601 tcggcccgtc ggtacggtca taaaaggctt ctgcgtctgc cagcgataac cctcccgcca
    21661 gcttgcgccc caccagacgc tcccgcaacg cagatgcggg agctttgata aagatcgaga
    21721 aatcacaaaa ttgcgcaagc tggcaccact tctcgtcgtc caacaacaac cagtttcctt
    21781 cgacgatgac gagtggtgcg gtaacgtgca acacatcttc aacaggatca tgcttttgtc
    21841 gatcgtactg cggccacgta caatcccctt ccacaacccg gcacagattt tccgccagtt
    21901 tcgcaacgtt gaatgtctct ggtgcgcctt tgaagggtcg caattgatgc acatccagcc
    21961 agctattgta atggtgaaaa ccatccatcg gcagcgtctg aatagcgggc agttccgggt
    22021 cttgttgcgc gagatattcc cagaaggtgg ttagggtaga tttacccgtc cccggcggag
    22081 cgcaaagaaa aaccaccgta cgccgctgcg gatttactgt ctgtaaggcc gccagcatac
    22141 gcagcaatgg tttatgtaca ttttcaattt catcatcaga gtactgcgcc tgtaccttta
    22201 gtccattcac cgtcagttcg attttcacgg cgttaattcc tttaaggtgg tcgttactgc
    22261 cagcgtcagc gccgttttga ccatcccgct aaaacgcgcc tctgaccaga cggcgaaatc
    22321 ggcgaacttc atcgctttca acgcgtcata gagcgtcgca ttgcgggtta tgctattgac
    22381 gttgctgata aaatcccagg ttatgtcatc agcaaatctg ggcgtttcat cctgttcgtt
    22441 cagataatgc caaaactggg aaaaatgcgt gatatcggcg tacagccatt tatccatttt
    22501 ccccagcgca taaatcaaac gcagcgcaac atcaatatcg tcaagcgggc cgctttgggc
    22561 gagtaacggc ttcactgcat attcgacgat ctcttcatcg ttatcgacaa acagcgacgg
    22621 cagaaactgg cgtatcccct ggagcaaaat agaatgcgca attttcataa atgaaaataa
    22681 attgtcctgt gcattcagtt gctcaagcac atcatcttct gtcagcgtcg ccattggtct
    22741 tccgatagat caccatcaat gcagggagtc tattatatta cacgtttggt ccgcgccgcc
    22801 gatcagtaat gtctgcgtcc gcaccccatt cgccgtctgt tggataccgt tcaccaactc
    22861 gccgcccgtc acgcccgtag cgctaaagag gatatcgttc cccctcgccg ataaccactc
    22921 gcccacgaaa agcaacgtcg tttaatgcct ggcgcatcgc ggtgaccgca aggccatcaa
    22981 ttttattttt gtcgccacag ccggtttgcg gccaggcagc caacgctgcc tgttccgtaa
    23041 cgcgaaataa tggccacgcc agggacatca ttgcgcggcc tccccaatat caacgccaaa
    23101 ttgtgccagc agatatttct cggcctgttc attccagata ccgtgcgttt tctcaaccag
    23161 ccacgccagc tctttaaaca gcggatcggt tttgcctttt tcggcaaaat cggcaatggc
    23221 ggtcagcggc atggtcacgc cgttataaat gagtttttta ccgccaggga tatccggcag
    23281 attgagcacg gtttctggca ccgcatccag gccaccgata tgggtcacca taaacgacgg
    23341 ctgtaactgc cccgtggcgc taagggcaat cgcctctttc atgtcgtccg tggaaccgcc
    23401 ggacgtgccg acaacgtgcg tactgttgta atggacgttg tagaaattaa acggcacttt
    23461 gaagttttta tccgtcggac cggcaaagaa gttcaggcag ccatcttccg ccagtaattc
    23521 atccgccatc tcgacgactg aaggcaccgc tgcatagaca aatacgtcat caaatccggc
    23581 atcatccgtc aacgcccgca gcgtctggac cggatcgctc atccctttag tattcatata
    23641 caccagctca atgcctttac tggccgccag ttccaccggc agcagcatct gcacctgcgc
    23701 cagccgtttg tcgtcgatat cgactaccac cacccgcgac ggttgtatgc cgccgttaat
    23761 ggcgtaatca atagcgccaa ttcccatcgg gcctgcacac gccagtagcg caatattgcc
    23821 gccaggtttg acgcccatac gatgctcata aacatattgc gtggtgtgat aattggcatg
    23881 ataagcgcca ataatgcagc acataggctc tgccagcgac gccgcagcaa aataagagcc
    23941 gtgatagggt aatacgcagc ccaaattaat ggcgatttcg ggaataatca tataggtggc
    24001 gttaccgcca aaatattcgt agctgtagcc cgctgaatac ccgcctggta accccatcgc
    24061 cggttgcaat acaaaacgct ggcctttttt atatttgcca gtgagatttt tacccacttc
    24121 gacaataacc ccggcacatt catgcccggt aatgaccgga tgattttcta aatcgtcggg
    24181 gacgcgttta tgttcactac cgagtaacgc cgctttccag gtggataaac aaacgctgtc
    24241 agaaattaca ttcactaaca gttcattatc ggtaatttct ggcagctcaa attcgcgcag
    24301 acggacatcc cgcttgccat aaatagcagc aactttggtt ttcattttta cctctgtaat
    24361 cagtgtttat gttcagcggt taattgatta aataaagtgt cgagttttgg atcgccaata
    24421 tagttattaa tcaatatcag tggtttatcc gtcacacgtt tcacgcgccc ttccagactg
    24481 gcgtgagtaa cgacgatatc cgcatccgca ggcacgttt
//
LOCUS       DAZFFE010000071        23954 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFFE010000071 DAZFFE010000000
VERSION     DAZFFE010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.071"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            180..818
                     /gene="leuE"
                     /locus_tag="ACHJ69_003034"
     CDS             180..818
                     /gene="leuE"
                     /locus_tag="ACHJ69_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO5015970.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSETKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            991..1350
                     /gene="yeaR"
                     /locus_tag="ACHJ69_003035"
     CDS             991..1350
                     /gene="yeaR"
                     /locus_tag="ACHJ69_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO5015971.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            1354..1536
                     /gene="yoaG"
                     /locus_tag="ACHJ69_003036"
     CDS             1354..1536
                     /gene="yoaG"
                     /locus_tag="ACHJ69_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO5015972.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            1684..1932
                     /gene="yeaQ"
                     /locus_tag="ACHJ69_003037"
     CDS             1684..1932
                     /gene="yeaQ"
                     /locus_tag="ACHJ69_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO5015973.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(1992..2066)
                     /gene="yoaJ"
                     /locus_tag="ACHJ69_003038"
     CDS             complement(1992..2066)
                     /gene="yoaJ"
                     /locus_tag="ACHJ69_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO5015974.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(2069..2167)
                     /gene="yoaK"
                     /locus_tag="ACHJ69_003039"
     CDS             complement(2069..2167)
                     /gene="yoaK"
                     /locus_tag="ACHJ69_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO5015975.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            complement(2200..3225)
                     /gene="dgcP"
                     /locus_tag="ACHJ69_003040"
     CDS             complement(2200..3225)
                     /gene="dgcP"
                     /locus_tag="ACHJ69_003040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO5015976.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCVAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     THSALHS"
     gene            3408..3662
                     /gene="yoaF"
                     /locus_tag="ACHJ69_003041"
     CDS             3408..3662
                     /gene="yoaF"
                     /locus_tag="ACHJ69_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO5015977.1"
                     /translation="MKIISFVLPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            complement(3684..4031)
                     /gene="yeaO"
                     /locus_tag="ACHJ69_003042"
     CDS             complement(3684..4031)
                     /gene="yeaO"
                     /locus_tag="ACHJ69_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO5015978.1"
                     /translation="MNIQCKRVYDPAEQSDGYRILVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4086..5267)
                     /gene="nimT"
                     /locus_tag="ACHJ69_003043"
     CDS             complement(4086..5267)
                     /gene="nimT"
                     /locus_tag="ACHJ69_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO5015979.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRYAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISQGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            5364..6170
                     /gene="nimR"
                     /locus_tag="ACHJ69_003044"
     CDS             5364..6170
                     /gene="nimR"
                     /locus_tag="ACHJ69_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO5015980.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKEPVEWGSLRQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALGD
                     TVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            complement(6127..6573)
                     /gene="yeaL"
                     /locus_tag="ACHJ69_003045"
     CDS             complement(6127..6573)
                     /gene="yeaL"
                     /locus_tag="ACHJ69_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO5015981.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            6743..6847
                     /gene="yoaI"
                     /locus_tag="ACHJ69_003046"
     CDS             6743..6847
                     /gene="yoaI"
                     /locus_tag="ACHJ69_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO5015982.1"
                     /translation="MNDQMFVETLIITSSFFAIAAVLVLSVLLIERTG"
     gene            complement(6848..7351)
                     /gene="yeaK"
                     /locus_tag="ACHJ69_003047"
     CDS             complement(6848..7351)
                     /gene="yeaK"
                     /locus_tag="ACHJ69_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO5015983.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(7394..8884)
                     /gene="dgcJ"
                     /locus_tag="ACHJ69_003048"
     CDS             complement(7394..8884)
                     /gene="dgcJ"
                     /locus_tag="ACHJ69_003048"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO5015984.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVPPEQRRAICEAFESANNTHGLNLTAHK
                     YQGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            complement(9065..10540)
                     /gene="cdgI"
                     /locus_tag="ACHJ69_003049"
     CDS             complement(9065..10540)
                     /gene="cdgI"
                     /locus_tag="ACHJ69_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO5015985.1"
                     /translation="MIRSTRISLGLFFNYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQGFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTHNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            complement(10687..11970)
                     /gene="yeaH"
                     /locus_tag="ACHJ69_003050"
     CDS             complement(10687..11970)
                     /gene="yeaH"
                     /locus_tag="ACHJ69_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO5015986.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESISIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKILPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(12083..14017)
                     /gene="yeaG"
                     /locus_tag="ACHJ69_003051"
     CDS             complement(12083..14017)
                     /gene="yeaG"
                     /locus_tag="ACHJ69_003051"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO5015987.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAEHYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            14453..15199
                     /gene="mipA"
                     /locus_tag="ACHJ69_003052"
     CDS             14453..15199
                     /gene="mipA"
                     /locus_tag="ACHJ69_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO5015988.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            15289..16143
                     /gene="yeaE"
                     /locus_tag="ACHJ69_003053"
     CDS             15289..16143
                     /gene="yeaE"
                     /locus_tag="ACHJ69_003053"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO5015989.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALIGLRENVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFALEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQKQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     HNISAAQVLLAWVISHQGVMAIPKAATVAHVQQNAAVLEVELSSAELVMLDKAYPAPK
                     GKTALDMV"
     gene            complement(16194..17078)
                     /gene="yeaD"
                     /locus_tag="ACHJ69_003054"
     CDS             complement(16194..17078)
                     /gene="yeaD"
                     /locus_tag="ACHJ69_003054"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO5015990.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGIPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFHVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(17162..18157)
                     /gene="gapA"
                     /locus_tag="ACHJ69_003055"
     CDS             complement(17162..18157)
                     /gene="gapA"
                     /locus_tag="ACHJ69_003055"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO5015991.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            18499..18912
                     /gene="msrB"
                     /locus_tag="ACHJ69_003056"
     CDS             18499..18912
                     /gene="msrB"
                     /locus_tag="ACHJ69_003056"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO5015992.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            18954..19226
                     /gene="yeaC"
                     /locus_tag="ACHJ69_003057"
     CDS             18954..19226
                     /gene="yeaC"
                     /locus_tag="ACHJ69_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO5015993.1"
                     /translation="MNFDDIINSMTPEVYQRLSTAVELGKWPDGVTLTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            19596..20672
                     /gene="ydjL"
                     /locus_tag="ACHJ69_003058"
     CDS             19596..20672
                     /gene="ydjL"
                     /locus_tag="ACHJ69_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO5015994.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCFVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFYVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            20699..22078
                     /gene="ydjK"
                     /locus_tag="ACHJ69_003059"
     CDS             20699..22078
                     /gene="ydjK"
                     /locus_tag="ACHJ69_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5015995.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            22095..23138
                     /gene="ydjJ"
                     /locus_tag="ACHJ69_003060"
     CDS             22095..23138
                     /gene="ydjJ"
                     /locus_tag="ACHJ69_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFO5015996.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLTMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            23159..23847
                     /locus_tag="ACHJ69_003061"
                     /pseudo
     CDS             23159..23847
                     /locus_tag="ACHJ69_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
     gene            complement(23865..>23954)
                     /locus_tag="ACHJ69_003062"
     CDS             complement(23865..>23954)
                     /locus_tag="ACHJ69_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO5015997.1"
                     /translation="LGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         6302 a         5513 c         5864 g         6275 t
ORIGIN      
        1 tacgccagta ttttgccgag cactacccga atttttcact ggagcatgcc tgattaatga
       61 ttcaattatc gggttgatat cgagttaaaa cctgattttc tcctttctaa gccgctacag
      121 attggttagc atattcacct ttaatcgcgc atgatcgaaa gataattaaa gaggttaatg
      181 tgttcgctga atacggggtt ctgaattact ggacctatct ggttggggcc atttttattg
      241 tgttggtgcc agggccaaat accctgtttg tactcaaaaa tagcgtcagt agcggtatga
      301 aaggcggtta tcttgcggcc tgtggtgtat ttattggcga tgcggtattg atgtttctgg
      361 catgggctgg agtggcgaca ttaattaaga ccaccccgat attattcaac attgtacgtt
      421 atcttggtgc gttttatttg ctctatctgg ggagtaaaat tctctacgcg accctgaaag
      481 gtaaaaatag cgagaccaaa tccgatgagc cccaatacgg tgccattttt aaacgcgcgt
      541 taattttgag cctgactaat ccgaaagcca ttttgttcta tgtgtcgttt ttcgtacagt
      601 ttatcgatgt taatgcccca catacgggaa tttcattctt tattctggcg acgacgctgg
      661 aactggtgag tttctgctat ttgagctttc tgattatttc tggggctttt gtcacgcagt
      721 acatacgtac caaaaagaaa ctggcgaaag tgggcaactc actgattggt ttgatgttcg
      781 tgggtttcgc cgcccgactg gcgacgctgc aatcctgatg ctttcagccc gcgttgccgc
      841 gggcttccca cctgtaattc tccctgattc ttctctgata cggtgctaaa aagtaaccaa
      901 taaatggtat ttaaaatgca aattatcagg cgtaccctga aacggctgga ataaaccgtt
      961 ttcagcgcat tcaccgaagg agggaaaagg atgctgcaaa tcccacagaa ttatattcat
     1021 acgcgctcaa cgcctttctg gaataaacaa actgcgcctg ccggaatatt cgaacgtcat
     1081 cttgataaag gaacgcgccc aggggtttac ccacgccttt ccgttatgca tggggcggtc
     1141 aaatatctcg gctacgctga tgaacacagt gcagagcctg atcaggtgat ccttatcgaa
     1201 gcggggcagt ttgcggtgtt ccctccagaa aagtggcaca acattgaagc catgactgac
     1261 gatacttatt tcaacattga cttcttcgtt gctcctgaag tcctgatgga aggtgcgcaa
     1321 caacggaaag tcattcataa cgggaaatga atcatgggca aagcaacgta taccgtgacc
     1381 gtcaccaata acagcaatgg cgtttctgtc gattatgaaa cagagacgcc gatgactttg
     1441 ctggtgccag aagtggcggc tgaagtgata aaagatctgg tgaataccgt gcgttcttat
     1501 gacacggaaa acgaacatga tgtttgtggt tggtaattat cactgatcgt tgatggcgca
     1561 atttctgcgc catcgcaaaa aattcctccg tgatgctcgt cacaaatctt accctcccga
     1621 tgttgtgcta tgctttttat cagcgactaa cccgctaatg caaaacagat aagtgaggaa
     1681 acaatgggca ttctgtcatg gattattttt gggcttattg ccggtattct ggcgaagtgg
     1741 atcatgccag gtaaagatgg tggtggattc tttatgacta tcctgctggg gatagtcggt
     1801 gccgtagtcg gcggatggat cagcacgctg tttggctttg gtaaagtcga tggcttcaat
     1861 tttggcagct tcgtggttgc cgttattggt gcgattgttg tgctatttat ctacaggaag
     1921 attaaaagtt aacgcgtaaa ttgcacaaag gctgcacaca ggcagccttt gctgtttttt
     1981 tagatgtgac gttaccacca tcccagctca gtgccgagta cgacaataat cgccacaccc
     2041 atcataataa tcgttgtttt tttcatcttt atgctcccgg ggcagcatag ccgccaataa
     2101 aaaaccatgc taaaaatacg accgctgtaa taaagattac aaccggaaaa ataataccga
     2161 ttcgcatgtt atcaccaatc aaaaggatgt gaatacgcct caggaatgta gcgctgaatg
     2221 cgtgacaaaa ggtgttttct gtttatgttt tttctcctga tacatcgcga tatcggcagc
     2281 atgcagggca ctgtctgcat cggtcgtttc aggatcgact tctactatgc caagactggc
     2341 accaggataa tacaaatcaa catcacctaa gtgatattct ccgcgtattt gttgttgaat
     2401 acgttctcgc agcgacgaaa tatccgcatt ctcgttgttc agtgaaacaa ccaaaaactc
     2461 atcaccgccg agacgaccaa taacttcgcc attttgctgg agcgtattaa ggcgctcgcc
     2521 aacctgaatg agaaacagat cgccactatt atgaccaaaa cgatcattga ttaatttgaa
     2581 gttatccaga tcgataaacg cgatcattat cttatggtta agatgccggg cgagagaaaa
     2641 cagcgtcgtc agattttcaa aaatcgcccg ccgattcggt agcccggtta acgagtcggt
     2701 atacgattgc gcaatcagcg cagcattagc ttcgcgcagc tgttcaacca gtgcctcttt
     2761 ttgaatatat tgtgcaatca gtccggcgaa caactgtaag acctgttccg cgcgttcact
     2821 ccactggcgc ttctcactgc tggcggcgca aagcgtgcca tagaatgatc catccggtaa
     2881 gtgaattggc gtgctcagaa atgtggtgat gccaagattg cgtgccgcaa cacagtcgcc
     2941 ccaacggtcg gggacttcat cgctgaaaaa acagttttca tcaatggcgc gtttgcataa
     3001 cgagtaatcc cacgagacgg taaaattctc cgggatgtgc attttctggc tattacgggc
     3061 aaacattata tgctgcaggc gcgcttcgac atccactttg gtcaggtagg ttgactccat
     3121 atcagtgacc atttccagca tctccagaag ctgtcggacc aggctttcca gtgactgttc
     3181 tttggcaagg gattgcgaga cgcgggcgat aatctgatct gacacgaaac aactccagga
     3241 tacggcgggt aaatgctgat tatttaatct ttgcaggcat gataaaacat catcaacaaa
     3301 gatgaaacaa ggcgtaatat gatttgagtg aatgtaataa aaatagtgct gcaaaacaaa
     3361 acgataccgc gatactctaa gcctggataa atatgtgaga tcaataaatg aaaattatca
     3421 gttttgttct gccttgcttg ctggttctgg ctggttgttc aacctcttct cagccagaag
     3481 cacctaaacc gccgcagatt ggtatggcaa atccggcgtc ggtctattgc cagcagaagg
     3541 gcgggacgct cattcctgtg cagacagcgc aaggggtcag caacaattgc aaattaccgg
     3601 gcggtgaaac gattgatgaa tgggcattgt ggcgacggga ccatccggct ggtgaaaaat
     3661 aaccgccgga tgctgtacta aaatcacaag ctacgtagcc agtctgccag caccagcgca
     3721 tggttctgcg tggtgttttt tgctgagtag agcagggtca gcggctgttt tttggcgatg
     3781 tctgccagcc gctttccttc ttgctcgtgt tgcgccagtt ctgcaagata ttgctcgcga
     3841 aaggttgcaa aatcgacgac ttcgccgtga aaggctttgc gcagctccgt tgacggcgtg
     3901 atttctttat cccactcatc aagggctaaa tcggtttttt tgataccgcg cggccagagg
     3961 cggtcgacca gtatgcgata accatcgctc tgttcagccg gatcataaac gcgtttgcac
     4021 tgaatattca tcgtgtttgt tctccctttc acgcatgatt tgtcacgtca ctatagcacc
     4081 ggatattagc gaatttcttt gtctctcccg gcgcaaagtc cgaaaatcgc catcagtagt
     4141 gaaagtatgg caacacccat cagtggtaca gaccagttac cgttagcatc gtgtatttta
     4201 cccatcagcg gcggcccaca ggctgccagc aaatacccga cggattgtgc catccccgag
     4261 agtgccgccg cctgatgcgc agaacttgcc cgcagaccaa tgaacgtcaa ccccagtatc
     4321 attgttgcgc cggaaccaaa accgaaaagc agagtccagg tgatcgcgtg tgctggcata
     4381 aagcagagcc caaccgcgcc aactgcgcac atcaaggcaa cgaacgctgc aataccacgc
     4441 tgatctttca catgatgtaa gaaaagtggg atcagcaaac cgggagctgc tgtggctagt
     4501 tgcagcaaac catgcagtga acccgcctgt gcttcgctat agccttgact gatgaggatc
     4561 gccggaagcc agccaataat cacgtaatag acgagtgagt tgatcccaag aaacaatgtt
     4621 acctgccagg caagcggcga acgccagata ccccgagtat gtaaggcgcg tgaggtactc
     4681 aaatttgcat gttgttgact tcgccactgt ggcagccata aaaaaagagc cagcagagga
     4741 aaacacatca gcatgagcaa cgcgccttgc cagccaaaac cgttcaaagc cagcggcact
     4801 accatagccg atcccagtgc cgccgcagct cccattgtca gggaatatgc gccggtaagt
     4861 ctggcgacgg aatgagggaa atcgcgttta attaatcctg gcagtaagac attgcctaat
     4921 gcaatcccac cgccaatgac cgctgtaccg ccaaataata agtaaggcga agggagagag
     4981 cgaattgcga tacctgcaca gatcaaaagt aacgcggcaa acaggctacg ttccatacca
     5041 aaacgtcgcg ccaccggggc ggccagcggt gagattagcg caaaggctaa taatggcagg
     5101 gtggtcaata agccggtttg cgccgtcgtt agcgagtaag cgtaacgaat cgtatccagt
     5161 aacggtgctg cgccggtaaa ggtgacgcgt aatgttgtgg caatcatcag aatgccagcg
     5221 ataaggacaa tcctgttttt gccgcttaat gaagttgaac aggtcatatg cttctcattg
     5281 ttacaaacga ggctgtacct taccgttttt attgttatgt tgaatcaagc taaaatgaca
     5341 aaatatcgct aaattcggac aaaatgatgc acaggttgaa tctcaatggc tatgaacccg
     5401 accgacacca tgaagccgcg gtcgcatttt gtattcacgc cggaacggat gagttaacca
     5461 gtcctgtaca tcagcaccgc aaagggcagt tgatactggc actacatggt gcaattactt
     5521 gtacggtgga aaatgctttg tggatggtgc cgccacaata tgcggtttgg ataccgggcg
     5581 gggtgaaaca tagtaatcaa gtgacagcta acgctgaact ctgttttctg tttatcgagc
     5641 ccagcgcagt gacaatgccg acaacatgtt gcacattgaa aatatcgccc ttatgccggg
     5701 aattaatttt aaccttggca aacaggacga cgactcagcg tgctgagccg atgacccgca
     5761 gattaattca ggtcctgttt gatgaactcc ctcagcaacc acaacaacag ctgcacttgc
     5821 ccgtttcttc tcatcctaaa atccgcacga tggtggagat gatggcgaaa gagcccgtcg
     5881 agtggggctc attgaggcaa tgggctggct tttttgcgat gagtgaacgc aatcttgccc
     5941 ggctgatagt gaaagagacg gggttgagtt ttcgccagtg gcgtcagcag ttacagctga
     6001 ttatggcgct tggcgataca gtacagaaag ttgctcatac actggggtat gattcaacaa
     6061 cggcctttat tactatgttt aagaaaggcc tggggcaaac gccaggccga tatattgcgg
     6121 gattaactac tgtttcccca caatcagcga aaccagaccc gcggcaataa gcgggccaac
     6181 cggtacgccg cgaaacagcg ccacgcctaa aacagtgccc accagcagcc cggcgaccag
     6241 ttgcggctgg ctgcccatta acgtcacgcc gcgccccccc agccaggaga caataacgcc
     6301 gactgcaatt gccaccagcg atttccagtt cagaaacgag tgaatcaggg ttgagggcgg
     6361 tagcgtcccg ctggcgatgg gggccataac gccaatcgtc agaatgatta tcccgatact
     6421 tagcccctgt ttttcaatcc acgggaaaaa ggtgcttaac ggtgtgacgc ggacaataat
     6481 caacaccaga attgaaacgg cgacagtggt gttatgactg ataaagccca gcgcagctaa
     6541 cccgagcagg atcagcaaag tgacatcaaa catcgtggtt tccttgccaa aaaataataa
     6601 gcctgcttac tttacgcttg ctgcaggcag gaagtagaaa atctttacgt tttttgcata
     6661 aaaaatcaga cgaactgtca tcaaactgcc atttgtgtac gttaacaata acgtgaaaac
     6721 agctatctcc cgggggcata ctatgaacga tcaaatgttc gtcgagacac tgattatcac
     6781 gtcatcgttt tttgccattg ctgctgtgct ggttttgtcc gttctgctta ttgaacggac
     6841 aggctagtta cgcagtgcgg cggaaattga ccagttctgg ttgcgcaata cgcaaataat
     6901 ccgcggtttt caaaataacg gatttatcca gcatgcctgc attgaaggcg atttcatcaa
     6961 atcgttcgaa gagtaggggg tcggcaacca gtttgagttt tggatggaag ctgaaagggg
     7021 ggatcgcgcc gaagacacag cctgtgagtt catcgacttc cgccgggctg gccaacgaag
     7081 cgcgtaatcc gcctatatgg ctggcgagtt ggctcagatc ggcctgttga tcggcagcga
     7141 ggatcgccag aacatgttga tttacgccat tgcctttgac tttgcatacc agtgccttag
     7201 ccccctggcc taaagcggtg ccacgaattt ctgacaccgc ttcacattta cctaccgctt
     7261 catgggtaac gacgcggaag ttagcacctt cttgtgataa taaagcgatt aaccgctgat
     7321 gcgtcacgct tcctttagcc atttcagtca tttcatgctc ctgagattac aagcaaacaa
     7381 ccacagaaga ttatcatgaa cggctgtttt tgttctgctt attgacatat aaacgctcat
     7441 cggaagcttt atacgcatca tgtaacgtat cgttttcttt catcgcgtaa ataccggaag
     7501 agaagccgat ctctttctgc ggcgcgatat gttgcagacg tttttcgata cgttcaggca
     7561 gttgtgctgc aatttgcggc gtcgaatcga caagaatgat gcagaattca tcgccaccga
     7621 gtcggatggc ataatcactt ttacgaatcg actgtttaat cgcctgagct aataacgtaa
     7681 tcgctaaatc cccctcctga tgaccgaggg tgtcatttat ttgctttaac ttgtccatgt
     7741 caatagcaat aaacatcacc gaagaaccgg attgcaccag tttctgcaac cgctgctcca
     7801 gttcaggggt taaaattttg cgattataga gtccagtcat cgcatcacta atattttctc
     7861 gcgacacatt ttggtataag cggaagtgca tccgcaccat attcagcaaa attaacgccg
     7921 tcaaaatcca gaacagaacg cttttccacg aagacgtgat aaagtaaaga atatcaatgg
     7981 ataacgagac acgaatgccg cccggtaagt catggacgta actgacatac tggaacagat
     8041 tatcttcgct ctggttgatg ataatgtcgc gccccgaatc tgtatcggtt agcgtgacat
     8101 tgagaaaacg ccagaggaga gggcggtcat gagtgtaaaa gatattccgc agattgtttt
     8161 tgttaatatc cagcagcaca atccctttta gctgccctgc gacatatacc ggcgtcagca
     8221 aactcatcac gttctgttta gtacgctgat cctggtaaat gctcgataat acggtacgcc
     8281 cggtaaatac tttttcaata tctttgcgat cgataccaat acttcctgac tgcaggaagg
     8341 accagttatt catcgcaaag tattcaacat taaccggctc ataaaaatag acatagcggt
     8401 cattgagatc gagataatag tgaaatttct cttcggccat cccaagacct gaaccgtaat
     8461 catcctggtg gcggttgcct tccactgcct gatcaaaagc gggtaatagt gctgcagctt
     8521 ccacaattgt gtcgcagtca gtggatgcgg tttgtagtgt gccgcgtaag ccctggtatt
     8581 tatgagcagt caggtttaag ccatgggtat tattggcaga ctcaaaagcc tcgcagatag
     8641 cgcgccgttg ttctggcggt acttccgact gctcagcagc gagagcgcgc atcacatgcg
     8701 cggcaatact ctgattctga tacttatcat aaagaaagga agagtctgct ctctgcacga
     8761 tatagcgcag ataggcactc attgctctgt cgctggcgac aagttcaaaa ataaggaagg
     8821 aagatgtcag cacaatgaca cttgcggcga taaaatgccg gagcattcta tggtgcaatt
     8881 tcatgataaa tggcactcct tcggttagtg aaacgagaaa accagataaa gttcgattgc
     8941 ggcctggcac aggggcgcta attatactca tctctgcgcc gctggggagg ggaaatttag
     9001 gggtaatagt aataaattgt atgcaaaagc cacgcaaaaa gcatggctgt gaatattttt
     9061 gcacttaaat atgatgaata atcactttgt tgcccccctc gcttttcgct tcgcgtaagg
     9121 ctttatctgc ttccgtcatg acaagcgaga tattatgcgt gtcacccgtt gaaaaaaccg
     9181 tgccaatact gatggtcatt tgttcaggaa catcatatct gttactaaag ccggtcagga
     9241 gttcgacatt tttacgcata cgttccgcaa tgatttttgc ctgggcacta ttgagttcgg
     9301 taaatagcaa gccaaacacc tcaccctcca gtcgcgctaa aatatcatcg gggcggatgc
     9361 tttgctggat aatatcgaca attgatacca gcactttatc acccacacga tgtccccatt
     9421 gggcattgat gcgtttgaac tgattaatat caaccagcat gacacaaaag tccttaacaa
     9481 caggtcgcga caataatgac tctacgctgt tgaaaaagta gcgccgatta taaatattag
     9541 tcagcacatc atgaactgcc agtttgctgg agagttgcag ttcctgaaaa atgttataaa
     9601 tcagaaaaga cacaacaaac agtttactgg aaacttcaat cccgcgactg atataccaga
     9661 tggtgtaatt atactcatcc agagtcagta gaagtaagtt gcagcatagc gatgccagac
     9721 ataataatgc tattaacggc catagttcgc tggctaatcg tgtacgcatg ataataaaga
     9781 acagtaaaaa tgcccacaaa caaaccaata tttttgtata gttgattccc caggtcgcag
     9841 tatggttgtc cggacagtaa tcgacgacat acaaagaata gtcagcgtta taactactca
     9901 gattgtgtgc cagaagggga aaaactaaaa aaggaatcag cgccagcaac agaatgccag
     9961 tttttttctt attattgtca aggatgttgt ctttcccata acaaaagagc gccagcgagg
    10021 ttaaacaaat gaaactgagt tggcggaaaa tatagaaaat agaaacatca ttcgactttg
    10081 tctggatcag tgtactgccg ttgattggtt tatggataat tacaatggtt tcaactaaat
    10141 agataaggcc acttaaaaat gcaaggctta aaatgagtgt tggtaaaatt tgtttgttag
    10201 tgaaatactt cattaacatg aaaagaaaaa tgacggcgtt aaggaaaaac aaagccacaa
    10261 caaccatcag gtaattcatc tgtgggataa gttttacatt tgtgttttcg ttatataagg
    10321 tagtaatgca aaataaaatt aacgtcagca ataagctgaa aataaaaaag ccttgagctt
    10381 ttaatttttg tgtacccata gaaaccataa aaaggagagc aatgtgagtg ctggatttta
    10441 ttgatggcag agatatatag ttttgaccag gatcaatttt cgttaacgat aaaaaataat
    10501 taaagaataa ccccagggaa atacgtgttg accgaatcat ttttccgaat gattgttttc
    10561 tggaactaaa ttgtgtaaag gttatgctgt catatttaaa gcgattgtaa gctaatgtat
    10621 gtaataaatg agataattta cagtgagcta aaaatcagcc aggcgataat gacccggctg
    10681 acagttttag tctttagctg ttgcattttg tttatgaaac agttcacgga acaccggata
    10741 aatatcatcc tggtcgcgga tatgctgcat cgcaaagttg tcgaaagtag attgcagatg
    10801 ctcatattct cgccacagtg tctgatgcgc gcgacgtgta atttcgatat agctgtaata
    10861 acgaacaacg ggtaatattt ttttcgccag aatttcatgg caaagcggag agtcatcggc
    10921 ccagttatcg ccgtctgatg cttgggctgc gtaaatattc cactgtgccg ggttataacg
    10981 ctctttcact acctcatcca tcagtttcag ggcgctggaa acaatggtgc caccagtttc
    11041 ctgcgagtag aaaaactcat gttcatcgac ttctttcgcc tgggtatgat ggcggatgta
    11101 tacgacttcc acgttcttat acgttctgct gaggaacaga tacagcagaa tataaaaacg
    11161 tttagccata tctttagtgg actgatccat tgaaccggaa acgtccatca ggcaaaacat
    11221 cactgcctgg ctggagggat cgggccgttt ttcgtagttc ttgtaacgta aatcgaaggt
    11281 gtcaatgaaa gggacgcgtt caattttggc acgtaattct gcaatttctt tgcgcagacg
    11341 ttcctcttcc agcagttgcg caggttcact gttgctgatg atggccaaat tctcttccag
    11401 cgcatgaagt tcccgccgct tgcccgccgt catagctgtg cgccgcgcca gtgagttctg
    11461 caatgaacgt acaacgctga tattggccgg aacgccgtta gcggtataac ccgcccgatg
    11521 cgttttatat tcggtcagct ggcgttgttg gttttgtttc agattcggta aggccaaatc
    11581 ttcaaagagc agatcaagat actcatcttt cgaaatctga aagacaaatt catcctgacc
    11641 ttcaccatcc tggctggcct ggccctgacc actgccggaa cctccgccac caccctgggg
    11701 acgttcaatt cggtcgttct ggacgaaatg gtcattgccc ggatgcacac ggtggcgcag
    11761 gccaccacgc ccctgatgaa acatcggttc gctaatatct tccgtgggaa tggatatgga
    11821 ctcgccgctg tcgacgtcag tcaccgaacg cttattaatg gcctcggaga tcgactgttt
    11881 aatttgcgct ttataacggc gtaagaaacg ctggcgattc accatgcttt tatttttgcc
    11941 gttcagacgc cggtcaataa accaggtcat atgccccccg tactacattt gccaacggta
    12001 tgctacgctt gtgttgccgc ttctctggcc cacggttcgc tgtcgttgta ggcccggtag
    12061 catgtgtacc gggccagggt tgttaagacg atttacgtac gcgcaaatac cattcgcaca
    12121 gtaaacgcac ctgtttacgg gtgtagcctt tttccatcat acggtcgaca aagtcgtcgt
    12181 gtttcttctg ctcgtcggtt gacgttttgg cgttaaacga aataaccggc aacagctctt
    12241 cggtattgga gaacattttc ttctcgataa ccgtgcgcag tttttcatag ctggtccagt
    12301 tcggattgcg tccgctgtta ttcgctctgg ctcgcagtac gaagttaaca atctcgttgc
    12361 ggaaatcttt tggattactg atccccgccg gtttctcgat tttctccagc tcggcgttaa
    12421 gagactcgcg gtcaaacaac tgcccggtat ccggatcgcg atactcctga tcctgaatcc
    12481 agaaatccgc gtaggtaaca taacggtcga aaatgttttg cccatattct gaataggatt
    12541 caaggtaggc cgtctggatc tctttgccga taaactcggc atatttcggg atcagataac
    12601 ctttcaggaa ctccagatag tgttcagcct gctcttgcgg gaactgctcg cgctcgatct
    12661 gctgttccag gacgtagaac agatggaccg ggtttgccgc cacttctaca tgatcgaagt
    12721 tgaacacgcg ggagaggatc ttaaacgcaa aacgcgtcga cagaccgttc atcccttcat
    12781 cgacaccggc gtagtcacga tattcctgat acgacttggc tttgggatca gtgtctttca
    12841 gactttcacc atcataaacg cgcatctttg aataaatgct ggagttttct ggctctttca
    12901 gacgcgaaag aatagaaaaa cgtgacagcg tttccagcgt gccaggggcg cagggggcgt
    12961 gagtcaattc actgtgatta agcaatttct cgtagatttt gatctcttcg gaaatgcgca
    13021 agcaatacgg caccttcacg atgtaaacac ggtcgaggaa ggcttcgttg tttttgttat
    13081 tacggaaagt gacccattcg gattcgttcg agtgggcgag aataatcccg ttgaacggca
    13141 gggcggagat accttccgtc ccgttgtagt taccttcctg ggtggcggtt aacaagggat
    13201 gcagcacttt tatcggtgct ttaaacatct cgacaaattc catgatcccc tgattggcgc
    13261 ggcacagcgc accggaatag ccgtaggcgt ccgggtcatt ctgcgcgtag tgttcgagtt
    13321 tacgaatatc gactttccct accagggcgg agatgtcctg gttgttctca tctccgggtt
    13381 ccgttttggc gatggcaatt tgttgcagaa ttgacggcca gaccttcact acccggaact
    13441 tagtgatatc gccaccaaat tcatgcaggc gctttgccgc ccacggcgac atgatggtgc
    13501 cgagataacg gcgaggaatg ccgtactctt tttccagaat ctgcgcatct tcctgcggat
    13561 tgaaaagaca gaacggatga tcgttgaccg ggctacgctc accgttggcg ctcaatacat
    13621 aaatcggtac gagctgcatt aatgatttca gtcgctcagc aagcgatgat ttacccccac
    13681 ccacaggccc cagcagatac aggatttgtt tcttctcttc cagcccctga gccgcgtgtt
    13741 tcagataaga aacaatctgt tcaatcgcgt cttccatgcc gtaaaactct tcaaacgccg
    13801 gataacgtgc aatgacccgg ttagaaaaga gtcgagaaag tctgggttcc tgggctgtat
    13861 cgaccatgac aggctcacca atagccatca atagccgctc agccgcgttg gcataagcac
    13921 tgcgatcttg ccgacaagtg gtaagaaact cctgcagtgt gaactcttcg tccttggcag
    13981 cttcatatcg ctggcgatag tgatcgaata tattcatggt atgccgtcct ttcgtttttt
    14041 agcacaggtt aagagccgtt catatgaata gtagaggctc ccggaagagg taaactgaac
    14101 gacgtctctt gctaaaacac agcaactctc atgccatttt attgacgatg agaacaacgt
    14161 gcccggtgat acaccttcaa aattaagcgt agatggcaaa tggaaaactt gcctgcacgg
    14221 ctttcttaat ttcaatggca tatcaacaac tcatccataa attttgcata attaatgtaa
    14281 ataatagggt cgtcggtaac gctaaattca tttggctgta agcgtggtgt catctgcgtc
    14341 aggaaaatta aacagttact ataaaaaatg aaaacgtaaa aaggttgggt ttcgatgtat
    14401 tgacgggtaa actttgtcgc ccgctaaaca tttgtttaag gaatgattaa ttgtgaccaa
    14461 actcaaactt ctggcacttg gagtgcttat cgcaacgtct gcaggcgtag cgcacgctga
    14521 aggtaaattt tccctgggcg caggcgtagg tgtcgttgaa cacccatata aagattacga
    14581 taccgatgtt tacccagtac cggtaatcaa ctatgaaggc gataacttct ggttccgtgg
    14641 cttaggtggt ggttactacc tgtggaatga cgcgacggac aaactttcaa ttaccgctta
    14701 ctggtcgccg ctttacttca aagctaaaga cagtggcgat caccaaatgc gtcacctcga
    14761 tgaccgtaag agcaccatga tggctggtct gtcttatgct cactttaccc agtacggtta
    14821 cctgcgtacc accctggctg gcgataccct ggataacagc aacggcatcg tctgggatat
    14881 ggcctggttg tatcgttaca ccaacggtgg cctgaccgtg actccgggta ttggtgtgca
    14941 gtggaacagc gaaaaccaga acgaatacta ttatggcgta tcgcgcaaag agtccgctcg
    15001 cagcggtctg cgtggctata acccgaacga cagctggagc ccttacctgg agctgagcgc
    15061 cagctacaac ttcctcggtg actggagtgt ttacggtacc gcgcgctaca cccgtctgtc
    15121 tgatgaagtt actgacagcc caatggtgga taaatcctgg actggcctga tttctaccgg
    15181 gatcacctac aaattctgat aatgcatttt tccagggcat tttgtgcata aaaacagggc
    15241 gtaatgccct gttttgcatt acggtggtgc aatacctggg aggaaacaat gcaacaaaaa
    15301 atgattcaat ttagtggcga tgtctcactg ccagccatag ggcagggaac atggtatatg
    15361 ggcgaagatg ccagtcagcg taaaacagaa gttgctgcac tacgcgcggg cattgaactc
    15421 ggtttaaccc tcattgatac cgccgaaatg tatgccgatg gcggtgccga aaaagtggtt
    15481 ggggaagcat taatcggtct gcgtgagaac gtctttctcg tctctaaagt ctatccgtgg
    15541 aatgctggcg ggcaaaaagc gataaatgca tgcgaagcca gtttacgccg tctcaatact
    15601 gattatctcg atctctactt attacactgg tctggcagtt tcgctctcga agagactgtc
    15661 gcagcgatgg aaaaattgat cgcccaggga aaaatccgcc gctggggcgt ttctaacctt
    15721 gattatgccg atatgcagga actctggcag ctgccggggg gaaatcagtg tgccactaat
    15781 caggtgcttt accatctcgg ttcacgaggg attgagtacg atctacttcc ctggtgccag
    15841 aaacagcaga tgccggtgat ggcttacagt ccattagccc aggccgggcg gttgcgcaat
    15901 ggactgttaa aaaacgcggt agtcaacgaa attgcacatg ttcacaatat cagtgcggca
    15961 caagtattgt tggcgtgggt aatcagccat cagggtgtga tggcgattcc aaaagcggca
    16021 actgtcgcac atgtccaaca aaatgcggct gtgcttgagg ttgaactttc gtcagcggaa
    16081 ttagttatgc tggataaagc gtatccggca ccaaaaggaa aaactgcgct ggatatggtg
    16141 tgatgttgat tcgtgccgga tgcatcgcac ccggcacaca atattaacgc aaattaacgt
    16201 ttagcaacgc gaatggattg cgccagatgt gcaggtttct ctttggtgac tttttgcgtt
    16261 tctgaagcgt aagccgtttc tacacaaaca aaggtcttgt agccatcatc cggcatatcg
    16321 cccatgctaa ctgaaagcgc cggtcctggg ttccagccta caacgttcag atgatgctgg
    16381 tggcctacgg cgataatacg attcagcgct tcgtcattaa ttacgctgca atcttgcgga
    16441 ttcagataca cgcgatcggt acggtcaggg aaggtctgaa taccgtcggt cagtacatct
    16501 tctttcgcgt catttacttt atcaatgaag cgatcgccta gtccactgac gcttacctta
    16561 gcgatatcac ccacgttaaa gtaggtatgc agagcagagg tagtttcaaa ttcgccgtgt
    16621 gcttcaagat cgatttcaca ggttttaccc acgtggaaat gcgctaacag cgtaaagtcg
    16681 tgcggccaga attttttcgt ctcttcgctt tgtgtcaatt caaaagtcag cgctacacca
    16741 tcagcatctt cacgatgtga tttcagcgtc cacggcaggt tgcgggcaaa accgtgcgca
    16801 ggcagacctt gttgtgcagc cggaccaaac cacggccagc aaaccggtat gccaccgcga
    16861 atggcgacgc catttttgaa cggtgtgttg ttactcaacc acagaacttc ttcttcaccc
    16921 gcaggtttcc acgagagaag gtgtgcgccc tgtaatgcaa aagaggcttt tacctgggga
    16981 tgatcgacca caatgaggtc cagttcatcc agtttacgac gggagaggac aggggagatt
    17041 tgttcgatga ccggaagggc aaaaattttc ttaatcatga cgcagtcctt taacttcatt
    17101 ttatcaggta aaaaaaagag cgaccgaagt cgctcttttt agatcacagt gtcatctcaa
    17161 cttatttgga gatgtgagcg atcaggtcca gaactttgtt ggagtaaccg gtttcgttgt
    17221 cgtaccagga taccagtttc acgaagttgt cgttcagagc gatacccgct ttagcatcga
    17281 acacggaagt gcaaacttcg ccgttgaaat cggtagatac tacgtcatct tcggtgtagc
    17341 ccagaacgcc tttcatttcg ccttcagcag cagctttaac ggcagctttg atctgctcgt
    17401 aagttgcagc tttttccaga cgaacggtca ggtcaactac ggatacgttc ggggtcggaa
    17461 cgcggaacgc cataccagtc agtttgccat tcagttctgg cagtacttta cctacagctt
    17521 tagcagcacc ggtagaagac gggatgatgt tctgggaagc gccgcggccg ccgcgccagt
    17581 ctttgtgaga cgggccatca acggttttct gagtagcggt agtagcgtga acggtggtca
    17641 tcagaccttc gatgatgccg aagttatcgt tgataacttt agccagcgga gccaggcagt
    17701 tggtggtgca ggatgcgttg gaaacgatgt cctggccagc atatttgtcg aagttagcgc
    17761 ctttaacgaa catcggagtg ttgtctttag acggaccagt cataaccact ttcttcgcac
    17821 cagcggtgat gtgtttacga gcagtttcgt cagtcaggaa cagaccagtt gcttcagcga
    17881 caacgtcaac accaacttcg tcccatttca ggttagccgg atcacgttca gcggtaacac
    17941 ggattttttt accgttaacg atcagatgac cgtctttcac ttcaacggta ccgtcgaaac
    18001 ggccgtgagt ggagtcatat ttcagcatgt atgccatgta atcagcgtct aacaggtcgt
    18061 tgattgcaac gatctcgatg tcagaacgtt tctgagcagc acggaaaaca atgcgaccga
    18121 tacggccaaa accgttgata cctactttga tagtcatata ttccaccagc tatttgttag
    18181 tgaataaaag gttgcctgta aaattacaaa aacctcacgc agcgtcaagc ggaatcgtgt
    18241 caatcattgc gacaaatcaa tcctgtgcct aagcattacg cgactgactc gcctcactct
    18301 tcctttgggc ttgagaccac atggggtcgg cgccccgaat tttaaagggc aattacgata
    18361 aaaatgtgat tttcatcacg atttaggtgc agccatttcg tcgagatcaa gtttaggaca
    18421 aaattttgcc agttggtgtt aatgttttgt tagaatcagt cacgtaatgt gagcacgatt
    18481 aaagtgagat gtgagcaaat ggctaataaa ccttcggcag aagaactgaa aaaaaatttg
    18541 tccgagatgc agttctacgt gacgcagaat catgggacag aaccgccatt tacggggcgt
    18601 ttactgcata acaagcgtga cggcgtatat cactgtttga tctgcgatgc cccgctgttt
    18661 cattcccaaa ccaagtatga ttccggctgt ggctggccca gtttctacga accggtgagt
    18721 gaagaatcta ttcgttatat caaagacttg tcacatggaa tgcagcgcat agaaattcgt
    18781 tgcggtaact gcgatgccca tctgggccat gtctttcctg acgggccgca gccaacgggc
    18841 gaacgttatt gtgttaactc tgcttcttta cgcttcactg atggcgaaaa cggcgaagaa
    18901 atcaacggtt gaaaaaacga ttcagcaact tattccacag gagcggaaaa attatgaatt
    18961 ttgatgacat tatcaacagc atgacgcctg aggtatacca gcgtttgtcg accgccgttg
    19021 aactggggaa atggcctgat ggcgttacgt tgaccgagga acaaaaagag aactgcctgc
    19081 aactggtgat gctgtggcaa gcccgccata ataccgaagc acagcatatg acaattgaca
    19141 caaatggtca gatggtcatg aagagcaaac agcagttaaa agaagatttt ggtatctcag
    19201 caaagcctat tgccatgttt aagtaagtgg tttatcccct ttttgccgcg gttataaagg
    19261 ggatagccaa caatgacccc acgactcatc tcaattatct cgccccgacc tggctaagtc
    19321 ccagcccgat cccttgcagt cgcgttcaca cctgaatgac ttctcctgaa cttaagtctc
    19381 aggcctattt ggccgggaat ccctctcgaa tttgctcttt ttgcgtcccg ccttccatca
    19441 acctgtaaca accccagcca ttttctgcgt aattgtgatc caagttgtaa aacgcagatc
    19501 attatctgtg ttttacagat ttaatgttga cctcaaaaat catgtgggta agtatgatga
    19561 ccatctaagc aaaacaaaac acagaggatg acgcaatgaa agcactggct cggtttggca
    19621 aggcctttgg tggctacaag atgattgatg tcccacaacc catttgtggc ccggaagatg
    19681 tcgtgattga aattaaagcc gcggcaatct gcggcgcaga catgaagcac tacaatgtcg
    19741 atagcggttc ggatgagttt aactctatcc gcggccatga gttcgcaggt tgtattgcgc
    19801 aggttggtga aaaagtcaaa gactggaaag tggggcaacg cgtcgtgtcg gataacagcg
    19861 gtcacgtttg cggcgtttgc ccggcctgtg aacaaggtga ttttctgtgt tgtacagaaa
    19921 aagtaaacct tggtctggat aacaatacct ggggcggtgg tttttccaaa tattgttttg
    19981 ttcctggtga aattctcaaa attcatcgtc atgcgttgtg ggaaatccct gatggtgttg
    20041 attatgagga cgcagccgta cttgacccta tctgtaatgc ctataaatcc atcgcgcagc
    20101 aatcgaaatt ccttccaggt caggatgtgg tcgtcatcgg cactggccca ctcgggctgt
    20161 tctacgtaca aatggcgcgg attatggggg cggtaaatat cgtcgtcgtt ggtctgcaag
    20221 aagatgtggc ggtccgcttc ccggttgcaa aagaactggg tgcgacggca gtagtaaatg
    20281 gttctaccga agatgtggtg gcgcgctgcc agcaaatttg tggcaaagac aatctggggc
    20341 tggtgattga atgctccggt gccaatatcg cattgaaaca agccatcgaa atgctccgcc
    20401 cgaatgggga agtagtacgc gttggaatgg gcttcaaacc tcttgatttc tcgattaatg
    20461 acattaccgc ctggaacaaa agcatcattg ggcatatggc ctatgactcc acttcatggc
    20521 gtaacgctat caggctatta gccagcggcg ctatcaaagt caaaccgatg atcacgcatc
    20581 gtatcggcct gtcgcaatgg cgcgaagggt ttgatgcgat ggtcgataaa actgcaatca
    20641 aagtgatcat gacttacgac tttgatgaat aactactctc ctgaaggaac gttatcgcat
    20701 ggaacagata acaaaaccgc attgtggtgc acggctggat cgcttaccgg attgccgctg
    20761 gcattcgtcg atgtttgcca tcgtcgcgtt tggcttgctt gtctgctgga gtaatgccgt
    20821 tggtggcttg atcctcgcgc agctgaaagc gttgggctgg acagataatt ccaccactgc
    20881 cacattctca gcaatcacga ccgccggaat gtttctcggt gcgctggtgg gcggcatcat
    20941 cggtgacaaa accggtcgca gaaatgcgtt tatcctctat gaggccattc atattgcctc
    21001 gatggttgtc ggtgctttct caccgaatat ggatttcctt attgcctgcc gttttgtgat
    21061 gggcgttggg ctgggagctt tactggttac actgtttgct ggtttcaccg aatatatgcc
    21121 cggaagaaat cgcggaacgt ggtccagtcg ggtttccttt attggtaact ggtcatatcc
    21181 gctctgttca ttgattgcga tgggactcac gccgctgatt agtgcagagt ggaactggcg
    21241 ggtacaactg cttatccctg caatattgtc acttatcgct acggcgctgg cctggcgcta
    21301 ctttcctgaa tccccgcgct ggctggaatc gcgcggacgg tatcaggaag ccgagaaagt
    21361 catgcggagt atagaagaag gcgtcatacg ccagacgggt aaacctttgc cgcccgtggt
    21421 tattgctgat gacggtaaag cgccacaagc ggtgccgtat tcagccttac tgacaggagt
    21481 attactgaaa cgcgtgatat taggctcttg tgtgctgatt gccatgaacg ttgtgcagta
    21541 cacactaatt aactggttgc caacgatatt catgacccag ggaattaatt taaaagactc
    21601 gattgtttta aataccatga gtatgtttgg cgcgccattt ggtattttta ttgccatgct
    21661 ggtgatggat aaaattccgc gtaaaacaat gggtgtgggg ctattaatcc tgattgcggt
    21721 gctcggatat atctattcac tgcaaaccag tatgttgctc ataacgctga ttggtttctt
    21781 cctgattact ttcgtctata tgtacgtttg ctatgcctcg gcagtgtatg tccctgagat
    21841 ctggccgaca gaggccaaac tccgtggctc cggtctggcg aatgcggtag ggcgaatcag
    21901 tggtattgcc gcaccttatg ccgttgcagt gctgctcagt agttatggcg taacgggagt
    21961 ctttattctt ctgggggctg tttcaattat tgtcgcaatt gctatcgcca ccattggaat
    22021 tgaaaccaaa ggtgtctccg ttgaaagttt aagtattgat gcagtagcca ataaataata
    22081 ctggtggatt caaaatgaaa aattcaaaag caatattgca ggtgccgggc acaatgaaaa
    22141 ttatttcagc agaaatacct gtgcctaaag aagatgaagt tttgattaaa gtggaatatg
    22201 tcggtatttg tggttcagat gtacatggtt ttgaatcagg cccgtttatt ccgcctaaag
    22261 acccaaatca agaaattggc ctgggtcatg aatgcgccgg gacggttgtg gctgtgggaa
    22321 gccgcgtgcg caaatttaaa ccgggggatc gggtaaatat cgaacctggc gttccttgcg
    22381 gtcactgtcg ttactgtctg gaaggcaaat ataacatctg cccggacgtt gattttatgg
    22441 cgacacaacc caactaccgc ggcgcattaa cgcactatct gtgtcatccg gagagcttta
    22501 cttacaaact gccagacaat atggacacga tggaaggggc gctggtggag cctgccgcag
    22561 tcgggatgca tgccgcgatg ctggcagatg ttaaaccggg taagaagata attattctgg
    22621 gagcgggttg tattggtttg atgacgttgc aagcgtgcaa atgcctggga gcaacggaaa
    22681 ttgccgtcgt tgatgtgctg gaaaaacgtc tgacaatggc ggaacagctc ggcgcgacag
    22741 tggttattaa cggcgcaaaa gaagacacta ttgcacgctg tcagcaattt accgaagaca
    22801 tgggcgcaga tattgttttt gaaacagcgg gttctgcggt caccgttaaa caggcacctt
    22861 atctggtcat gcgcggcggt aaaattatga ttgttggtac tgtacccggc gattcggcaa
    22921 tcaatttcct caaaatcaat cgcgaagtca ctatccagac ggtattccgc tatgccaatc
    22981 gttatccggt cacgattgaa gctatttctt cagggcgatt cgatgtgaaa tcgatggtga
    23041 cgcatattta cgattatcgg gatgtacaac aggcatttga agagtcagtt aacaacaaac
    23101 gcgacattat taaaggcgtt attaaaatta gcgattaatt aatacgggag aacagaacat
    23161 gctcgcagat atcagatatt gggaaaacga tgccacaaat aaacattatg tgcaattgcc
    23221 cattttaacg tatggaatgc agaaatgttg atgggcgtta tcgacgcagc cgaagaagcg
    23281 aaatcaccgg ttattatttc ttttggctca ggttttgtcg gcaacacctc atttgaagat
    23341 ttctctcaca tgatggtatc aatggcacaa aaagcaacgg tgccggtaat aactcattgg
    23401 gatcatggtc ggagtatgga gattattcat aacgcctgga ctcatggtat gaattcatta
    23461 atgcgtgatg cttctgcatt tgatttcgaa gaaaatattc gtttaaccaa agaggctgtt
    23521 gatttcttcc atccgctggg tattccggta gaggcggaat tagggcatgt cggtaatgaa
    23581 accgtttatg aggaagcgct ggcgggctat cactataccg atcctgacca ggcggctgaa
    23641 tttgttgaac gtacgggctg tgactcattg gctgtcgcca tcggcaacca gcatggggtt
    23701 tatacgtcag agccacaatt gaactttgag gtcgtcaaac gcgtacgcga tgctgtttct
    23761 gttccgctgg ttttgcatgg cgcatcgggg atcagtgatg ccgacattaa aactgcaatt
    23821 tcgttgggta ttgcgaaaat caacattcag gtaatgactc caacttattg atagtgtttt
    23881 atgttcagat aatgcccgat gactttgtca tgcagctcca ccgattttga gaacgacagc
    23941 gacttccgtc ccag
//
LOCUS       DAZFFE010000072        23673 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFFE010000072 DAZFFE010000000
VERSION     DAZFFE010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.072"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(343..1140)
                     /gene="ybcM"
                     /locus_tag="ACHJ69_003063"
     CDS             complement(343..1140)
                     /gene="ybcM"
                     /locus_tag="ACHJ69_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5015998.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSIFRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGISFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1150..1701)
                     /gene="ybcL"
                     /locus_tag="ACHJ69_003064"
     CDS             complement(1150..1701)
                     /gene="ybcL"
                     /locus_tag="ACHJ69_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFO5015999.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKRDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(1870..2202)
                     /gene="emrE"
                     /locus_tag="ACHJ69_003065"
     CDS             complement(1870..2202)
                     /gene="emrE"
                     /locus_tag="ACHJ69_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFO5016000.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGVFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2547..2861)
                     /gene="fliE"
                     /locus_tag="ACHJ69_003066"
     CDS             complement(2547..2861)
                     /gene="fliE"
                     /locus_tag="ACHJ69_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO5016001.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            3076..4734
                     /gene="fliF"
                     /locus_tag="ACHJ69_003067"
     CDS             3076..4734
                     /gene="fliF"
                     /locus_tag="ACHJ69_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO5016002.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPTNQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEALTREAMGFSEKRGDSLNVVNSPFNSSDESGGALPFWQQQVFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAVKTVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            4727..5722
                     /gene="fliG"
                     /locus_tag="ACHJ69_003068"
     CDS             4727..5722
                     /gene="fliG"
                     /locus_tag="ACHJ69_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO5016003.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5715..6401
                     /gene="fliH"
                     /locus_tag="ACHJ69_003069"
     CDS             5715..6401
                     /gene="fliH"
                     /locus_tag="ACHJ69_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO5016004.1"
                     /translation="MSDNLPWKTWMPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            6401..7774
                     /gene="fliI"
                     /locus_tag="ACHJ69_003070"
     CDS             6401..7774
                     /gene="fliI"
                     /locus_tag="ACHJ69_003070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO5016005.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7793..8236
                     /gene="fliJ"
                     /locus_tag="ACHJ69_003071"
     CDS             7793..8236
                     /gene="fliJ"
                     /locus_tag="ACHJ69_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO5016006.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            8233..9360
                     /gene="fliK"
                     /locus_tag="ACHJ69_003072"
     CDS             8233..9360
                     /gene="fliK"
                     /locus_tag="ACHJ69_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO5016007.1"
                     /translation="MIRIAPLITADVDNATLPGGKASDAAQDFLALLSEALTGEATTD
                     KAAPQLLVATDKPTAKGEPLVSDIVSDAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TITLAAAADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TVFTKLTSAQLTTAQPDDAPGTPAKPLTPLVAEAQSKAEVISTPSSVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            9465..9929
                     /gene="fliL"
                     /locus_tag="ACHJ69_003073"
     CDS             9465..9929
                     /gene="fliL"
                     /locus_tag="ACHJ69_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO5016008.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            9934..10938
                     /gene="fliM"
                     /locus_tag="ACHJ69_003074"
     CDS             9934..10938
                     /gene="fliM"
                     /locus_tag="ACHJ69_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO5016009.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10935..11348
                     /gene="fliN"
                     /locus_tag="ACHJ69_003075"
     CDS             10935..11348
                     /gene="fliN"
                     /locus_tag="ACHJ69_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO5016010.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            11351..11716
                     /gene="fliO"
                     /locus_tag="ACHJ69_003076"
     CDS             11351..11716
                     /gene="fliO"
                     /locus_tag="ACHJ69_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO5016011.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            11716..12453
                     /gene="fliP"
                     /locus_tag="ACHJ69_003077"
     CDS             11716..12453
                     /gene="fliP"
                     /locus_tag="ACHJ69_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO5016012.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGINSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            12463..12732
                     /gene="fliQ"
                     /locus_tag="ACHJ69_003078"
     CDS             12463..12732
                     /gene="fliQ"
                     /locus_tag="ACHJ69_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO5016013.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12741..13526
                     /gene="fliR"
                     /locus_tag="ACHJ69_003079"
     CDS             12741..13526
                     /gene="fliR"
                     /locus_tag="ACHJ69_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO5016014.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            13816..14439
                     /gene="rcsA"
                     /locus_tag="ACHJ69_003080"
     CDS             13816..14439
                     /gene="rcsA"
                     /locus_tag="ACHJ69_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO5016015.1"
                     /translation="MSTIIMNLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(14483..14671)
                     /gene="dsrB"
                     /locus_tag="ACHJ69_003081"
     CDS             complement(14483..14671)
                     /gene="dsrB"
                     /locus_tag="ACHJ69_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO5016016.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            14834..15061
                     /gene="yodD"
                     /locus_tag="ACHJ69_003082"
     CDS             14834..15061
                     /gene="yodD"
                     /locus_tag="ACHJ69_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO5016017.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            15359..16174
                     /gene="yedP"
                     /locus_tag="ACHJ69_003083"
     CDS             15359..16174
                     /gene="yedP"
                     /locus_tag="ACHJ69_003083"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO5016018.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVAVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAN"
     gene            complement(16171..17865)
                     /gene="dgcQ"
                     /locus_tag="ACHJ69_003084"
     CDS             complement(16171..17865)
                     /gene="dgcQ"
                     /locus_tag="ACHJ69_003084"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO5016019.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSGALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(18036..18218)
                     /gene="yodC"
                     /locus_tag="ACHJ69_003085"
     CDS             complement(18036..18218)
                     /gene="yodC"
                     /locus_tag="ACHJ69_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO5016020.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(18297..19214)
                     /gene="yedI"
                     /locus_tag="ACHJ69_003086"
     CDS             complement(18297..19214)
                     /gene="yedI"
                     /locus_tag="ACHJ69_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO5016021.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            19387..20307
                     /gene="yedA"
                     /locus_tag="ACHJ69_003087"
     CDS             19387..20307
                     /gene="yedA"
                     /locus_tag="ACHJ69_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO5016022.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFFAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(20296..20766)
                     /gene="vsr"
                     /locus_tag="ACHJ69_003088"
     CDS             complement(20296..20766)
                     /gene="vsr"
                     /locus_tag="ACHJ69_003088"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO5016023.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     DLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(20747..22165)
                     /gene="dcm"
                     /locus_tag="ACHJ69_003089"
     CDS             complement(20747..22165)
                     /gene="dcm"
                     /locus_tag="ACHJ69_003089"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO5016024.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSVWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISKCFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(22232..22927)
                     /gene="yedJ"
                     /locus_tag="ACHJ69_003090"
     CDS             complement(22232..22927)
                     /gene="yedJ"
                     /locus_tag="ACHJ69_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO5016025.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKDHPQRQRSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(22967..23248)
                     /gene="drpB"
                     /locus_tag="ACHJ69_003091"
     CDS             complement(22967..23248)
                     /gene="drpB"
                     /locus_tag="ACHJ69_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFO5016026.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         5859 a         6147 c         5916 g         5751 t
ORIGIN      
        1 ttaataatac ccagtgaaca attaacgtat caactagatt taataacaac gatatccgta
       61 cctaacctga tatctaatgt cataagtatc cctttaatta tcccctcaac ctcttgtaac
      121 tttcccactc ttgagctcca catagtccaa acgccggatg caaaaaaatc acacgtgcat
      181 ttctgaaatt gtgatggagg aagatagtat caattatatg tgcttataaa cgtccccgat
      241 tagcgcagaa gtcaccggat tgttcaggct ccaataggtt gagtatgtcg tatttatttt
      301 ccaaaataaa aatcgatgct gttgctcctt cataaaatac ttttactctc tggacgcttt
      361 tcttagctct cttggtgtta tcttaaaacg attatggaac ctttcagtaa aacgagaagg
      421 acacttgtaa ccattttccc tggcaatctc gctgatgggt tttactgtcg tttgtatagc
      481 agacaacgca ttatttaacc tcacgtcgtc caatatactt tgaaaactta ttccctcgct
      541 tgccagacgg cgatgcaatg tggaaacaga aatgtagaga tatctcgcta ccttgtttgc
      601 tgtccatttt gttccaggtt cggatagcat taggttataa caacgactta ttaatgattg
      661 tttactatct gataaaagta aacaattaac atgatgtgct cctaatgaaa gtagaacgcc
      721 cattgctaag tgttcctgaa tttgcgttga gaagccgcgg aaaatagatg tttttagttg
      781 ctcccaacaa tatattaact cggggttctg aggtaagcag aaacctgtcg tgttacgaat
      841 ttgatcagtt atcgcataaa atttttgaaa cttttcaatt aaatcaatgg gataataaag
      901 catttctgca agataaagac ctgcttctgg ataattcgca atataaaatt catatccaca
      961 gggaaataat attatctgat tgttatcaac agttagacta tgcgtctccc aattgataac
     1021 tttttttccc tgacggatac gacataaagc tggcataaga ggcttaaccc tgtgaatctc
     1081 atgatgttta tgcatccgta tttcttcaat ctttaggtta gacttatctt ttgccagcat
     1141 actcttaccc tactttatct cataaactgg tgttatctca gcggttgcaa ttttattggc
     1201 attaagcata taaccaacta acgctccgct ggaattagaa tccacaggaa tcttatcagt
     1261 ttttagagcc catactttaa actggtaatg atggggttta tctcttttgg gaggacatgc
     1321 tccaccaaat cctgcataac caaaatcatt tcggccttga acagcaccag tcggaagttt
     1381 tgtaccatca cgtcttcctg catcagtggg caaatatgtt acagttgctg gaatattagc
     1441 aacagtccag tgccaccatc cactgcctgt aggagcatct ggatcataca cagttacggc
     1501 aaagcttttg gtgccttcag gagcaccaga ccaggttaat gagggcgatg tattaccccc
     1561 ttcacaccca aatccagaaa atacatgaga cgttgtaagt tgctctcctg tttttatttc
     1621 attactagtg acctgaaatg ctgcggcctg cgcagaaaat gttatgaatg ccaatacact
     1681 tgaaacgata agttttttca taaaaacctc attgttgtga cctatcgtta ttttatttga
     1741 tgtgccttga tctcattatg cataaaggcg caatgatcgc gcaaatgcaa tcataattat
     1801 aaaggtgccc cgaacattag ccataatatg cacaaatcag attcctgtta tctcaaggat
     1861 aattattttt taatgcggtg cacttcgtga caacaagtta atgactaaca caccggcaca
     1921 aatcaacatc atgcctatga tagctggcag gtccagtcgt tgaccaaaaa ccccccagga
     1981 aagcaaacta atcagaacga taccgactcc tgaccagata gcataagcaa tccctgtagg
     2041 tatataagcc agcgtctgag ctaataacca gaatgatgca caatagcaaa taattgtgcc
     2101 aacagagggc cataaccgtg taaacccctc tgaaaacttc attaaggttg taccaatgac
     2161 ctctgcaagt attgcgccac caagataaat ataagggttc atagcatatt ctttcctgtt
     2221 catactggag agaattgtac tacagtttga acacaactca ccgttttcat cctggtgtat
     2281 gtgtatccag ggggctctgg cgtggcaaga gataaaggca ggaacaataa tgatcagaag
     2341 gaaccccagg ctaacgaatg agttcactct tcctgggagc aaagcgaaga caaaacatgt
     2401 tatcgcgttt acggatcgtg gatggcagaa aacaaccagg atcaggtatc cattttaaac
     2461 cagaaattga gtgagtttgc cccatccatg ccccacgctg tgggatcgga tataattaaa
     2521 caagcatagt caacaggtta cacaacctac acctgcatgg acatcacttc ctgatacgcc
     2581 gccaccagct tattacgcac ctgaatcccc atttgcatag aaactgaggc tttttgcata
     2641 tcggtcatca catcgtttag cgccacgccg ggttcaccga gggtgaattt ttctgcctgc
     2701 gtgcgggcga ctgtttgtgt atcgcttatg cgatcgagcg cggcgtgcag ctgcccggca
     2761 aaactaatgg tcggttgcgg cagtgattcc tgcgcacgcg cactcatcgc cgtagcctgt
     2821 aactggctga taaccccttc aatcccctgt atcgctgaca ttttcgtctc ccggataatt
     2881 tctggtagca aagcctacca gtaagtcaat aagacaaagg cgctaaatag caacaaaaaa
     2941 acgggtttat tggcggatag aaaaaaacga aagcacaaat aatgggagcg tcaatttttc
     3001 gagtttgctg acccgggagt gagtcttgtt ccactttgcc aataacgccg tccataatca
     3061 gccacgaggt gcgcgatgaa tgcgactgca gcccagacaa aatctcttga gtggcttaat
     3121 cgcctgcgtg cgaatccgaa aattccattg attgttgccg gttccgcagc agtggcggtc
     3181 atggtcgcac tgatcctgtg ggcgaaagcc cccgactacc gcacattatt cagcaatctt
     3241 tccgatcagg atggtggcgc aattgtcagc caactgacgc aaatgaatat tccttaccgc
     3301 ttcagcgaag ccagcggcgc tattgaggtt ccggcagata aagttcacga actgcgcctg
     3361 cgcctggcgc aacaaggttt gcccaaaggc ggcgcggtcg gtttcgaact gctcgatcag
     3421 gaaaagtttg gtatcagcca gttcagcgaa caggtgaatt atcagcgggc gctggaaggc
     3481 gagctttctc gtaccatcga aactatcggc ccggtaaaag gggcgcgcgt acatctggca
     3541 atgccgaaac cgtctttatt cgtccgtgaa caaaaatccc cttctgcatc ggtgacggta
     3601 aatctgttac ccggccgcgc actcgatgaa gggcaaatta gcgctattgt acatctggtt
     3661 tccagcgcgg ttgctggtct gccgccagga aacgtcacgc tggtggatca gggcgggcat
     3721 ctgttaaccc agtccaatac cagcgggcgc gatcttaatg acgctcagtt gaaatatgcc
     3781 agcgatgtcg aaggccgtat tcagcggcgt attgaagcga tcctgtcgcc tattgttggt
     3841 aacggtaata ttcacgccca ggtcacggcg caactggact tcgccagtaa agaacaaacg
     3901 gaagaacagt atcgccctaa cggtgatgaa tctcatgcgg cgcttcgttc acgccagctt
     3961 aatgagagcg agcaaagcgg ttccggttat ccgggcggcg taccgggggc gttgtcgaat
     4021 caaccggcac ctgcgaataa cgcgccaatc agcacgcctc cgacaaatca aaataaccgc
     4081 cagcagcagg cgagcaccac cagcaatagt gggccgcgta gcacacagcg gaatgaaacc
     4141 agtaactacg aagtcgatcg caccattcgt cataccaaaa tgaacgtggg cgatgtgcaa
     4201 cgtctgtcag tcgcggtagt ggtgaattac aaaaccttgc cagacggtaa accattgcct
     4261 ctcagcaacg aacagatgaa gcaaattgaa gctctgaccc gcgaggcgat gggcttttct
     4321 gaaaaacgcg gtgattcgct caatgttgtt aactcgccgt tcaatagcag tgacgaaagc
     4381 ggcggagcac tgccgttctg gcaacagcaa gtgtttatcg atcagttgct ggctgccggt
     4441 cgctggttgc tggtactgct ggttgcgtgg ttgctgtggc ggaaagcggt acgcccgcag
     4501 ctaacacgtc gcgccgaggc ggtgaagact gtgcagcaac aggcgcaggc ccgcgaggaa
     4561 gtggaagatg cggtggaagt ccgtctgagc aaagacgaac aactccaaca acggcgcgct
     4621 aaccaacgac tgggggcaga agtcatgagc cagcgtatcc gtgaaatgtc tgataacgat
     4681 ccgcgcgtgg tggcgctggt cattcgccag tggataaata acgatcatga gtaacctgac
     4741 aggcaccgat aaaagcgtca tcctgctgat gaccattggc gaagaccggg cggcagaggt
     4801 gttcaagcac ctctcccagc gcgaagtgca aaccctgagc gctgcaatgg cgaacgtcac
     4861 gcagatctcc aacaagcagc taaccgatgt gctggcagag tttgagcaag aagctgaaca
     4921 gtttgccgca ctgaatatca acgccaacga ttatctgcgt tcggtattgg tcaaagctct
     4981 gggtgaagaa cgtgccgcca gcctgctgga agatattctc gaaacccgcg ataccgccag
     5041 cggtattgaa acgctcaact ttatggagcc gcagagcgcc gccgatctga ttcgcgatga
     5101 gcatccgcaa attatcgcca ccattctggt gcatctgaag cgcgcccagg ccgccgatat
     5161 tctggcgttg ttcgatgaac gtctgcgcca cgacgtgatg ttgcgtatcg ccacctttgg
     5221 cggcgtgcag ccagccgcgc tggccgaact gaccgaagta ctgaatggct tgctcgacgg
     5281 tcagaatctc aagcgcagca aaatgggcgg cgtgagaacg gcagccgaaa ttatcaacct
     5341 gatgaaaacg cagcaggaag aagccgttat taccgccgtg cgtgaattcg acggcgagct
     5401 ggcgcagaaa atcatcgacg agatgttcct gttcgagaat ctggtggatg tcgacgatcg
     5461 cagcattcag cgactgttgc aggaagtgga ttccgaatcg ctgttgatcg cgctgaaagg
     5521 ggccgagcag ccactgcgcg agaaattcct gcgcaatatg tcgcagcgtg ccgccgatat
     5581 tctgcgcgac gatctcgcca accgtggtcc ggtgcgtctg tcgcaggtgg aaaacgaaca
     5641 gaaagcgatt ctgctgattg tgcgccgcct tgccgaaact ggcgagatgg tgattggcag
     5701 cggcgaggat acctatgtct gataatctgc cgtggaaaac ctggatgccg gacgatctcg
     5761 cgccaccaca ggcggagttt gtgccaatgg tcgagccgga agaaaccatc attgaagagg
     5821 ccgaacccag ccttgagcag caactggcgc aactgcaaat gcaggcccac gagcaaggtt
     5881 atcaggcggg gattgccgaa ggtcgccagc aaggtcatga gcagggctat caggaaggcc
     5941 tggcccaggg gctggaacaa ggtctggcag aggcgaaggc tcaacaagcg ccaattcatg
     6001 cccggatgca gcaactggtc agcgaatttc aaactaccct tgatgcgctt gatagtgtga
     6061 tagcttcgcg cctgatgcag atggcgctgg aggcagcacg tcaggtgatc ggtcagacac
     6121 caacggtgga taactcggca ctgatcaaac agatccaaca gttgttgcag caagaaccgt
     6181 tattcagcgg gaaaccacag ctgcgcgtac acccggatga tctgcaacgt gtggatgata
     6241 tgctcggcgc aactttaagt ttgcatggct ggcgcttgcg gggcgatccc accctccatc
     6301 ctggcggctg taaagtctcc gccgatgaag gcgatctcga cgccagtgtc gccactcgct
     6361 ggcaagaact ctgccgtctg gcagcaccag gagtggtgta atgaccacgc gcctgactcg
     6421 ctggctaacc acgctggata actttgaagc caaaatggcg cagttgcctg cggtacgtcg
     6481 ctacgggcga ttaacccgcg ctaccgggct ggtgctggaa gccaccggat tacaattgcc
     6541 gctcggcgca acctgtgtca ttgagcgcca gaacggcagc gaaacgcacg aagtagaaag
     6601 cgaagtcgtt ggctttaacg gtcaacggct gtttttaatg ccgctggagg aagtcgaagg
     6661 tgtcctgccc ggcgcgcgtg tttatgccaa aaacatttcg gcagaagggc tgcaaagcgg
     6721 caagcagttg ccgctcggtc cggcgttatt aggtcgcgtt ctggacggca gcggtaaacc
     6781 gcttgatggc ctcccctccc ccgatacgac ggaaaccggt gcgctgatta ccccgccatt
     6841 taacccgttg caacgtacac cgattgaaca tgtgctggac accggcgtgc gcccaatcaa
     6901 tgccctgctt accgtcgggc gcgggcagcg tatggggctg tttgccgggt ccggcgttgg
     6961 taaaagtgtg ctgctgggga tgatggcacg ttacacccgc gccgatgtca ttgtcgtggg
     7021 tttgattggt gaacgagggc gcgaagtaaa agattttatt gagaatatcc tcggtgccga
     7081 aggacgtgca cgctccgtgg tgattgccgc tccggcggat gtttctccgc tcctgcgaat
     7141 gcagggtgcc gcctatgcca cgcgcattgc cgaagatttt cgcgatcgtg gtcagcatgt
     7201 gttgctgatt atggactccc tcacccgcta cgcgatggcc cagcgtgaaa ttgcgctggc
     7261 gattggcgaa ccacctgcca ctaaaggtta tccaccgtcg gtgtttgcca aattaccggc
     7321 actggtcgag cgtgccggaa atggcattag cggcggcggc tcgattaccg cgttttatac
     7381 cgtgctcacc gaaggcgatg accagcagga tcccattgct gactccgcgc gggcaatcct
     7441 cgacggtcac attgtgctgt ctcgccgact ggcggaagcc gggcactatc cggctatcga
     7501 tattgaagcg tcgatcagtc gcgcaatgac ggcgttgatc agtgagcaac attacgcgcg
     7561 agtgcgcacc ttcaaacagc tgttgtcgag ttttcagcgt aaccgcgatc tggttagcgt
     7621 cggcgcgtat gccaaaggca gcgatccgat gctcgataaa gccatcgccc tgtggccgca
     7681 gctggagggc tatttgcaac aaggcatttt tgaacgcgcg gactgggaag cgtctctcca
     7741 ggggctggag cgtattttcc cgacagtgtc ataacccagg agataacggc agatggcgga
     7801 acatggtgcg ctggcgaccc tgaaagatct ggcagaaaaa gaggtagagg atgccgcgcg
     7861 cctgctgggt gaaatgcgtc ggggatgtca gcaggcggaa gaacagctca aaatgctgat
     7921 cgattatcag aatgaatatc gcaataacct caatagcgat atgagtgccg ggatgaccag
     7981 caaccgctgg atcaactatc agcagtttat ccagacgctg gaaaaagcca ttactcagca
     8041 tcgccagcaa cttaatcagt ggacgcagaa agttgacatt gccctgaaca gctggcgaga
     8101 aaagaaacaa cgtttgcagg cctggcagac actgcaggaa cggcaatcca cggcggcact
     8161 gcttgcagaa aaccgcctcg atcagaaaaa gatggatgag ttcgcccagc gcgccgccat
     8221 gaggaaacct gaatgattcg catagcgccc ttgattaccg ctgacgttga taacgccaca
     8281 ttgcctggcg gcaaagccag cgatgctgca caagattttc tcgcgttgtt gagcgaagca
     8341 ttaacaggcg aggcaacaac cgacaaagcg gcccctcagt tgctggtggc aacagataag
     8401 cccacggcaa aaggcgagcc gctggtcagc gatattgttt ccgacgcgca acaagcagat
     8461 ttactgatcc ctgtggatga aacaccgcct gtcatcaacg acgaacaatc cacatcaaca
     8521 ccgttaacca ccgctcagac gataacgttg gctgcggcgg ctgacaacaa tacggcaaaa
     8581 gacgaaaaag cggatgatct gaatgaagac gtcaccgcaa gcctgagcgc cctttttgcg
     8641 atgttgccgg gttttgacaa tacgcccaaa gtgactgatg cgccgtcaac cgtgttaccg
     8701 gcagagaaac caacggtctt cacaaaactg acttctgcgc aactcactac agcacagcct
     8761 gatgacgccc ccggcacacc agctaagcca ttaacaccgc tggtagcaga agcccagagt
     8821 aaagcggaag tcatcagcac gccttcgtcg gtgaccgctg ccgccagccc gctaatcact
     8881 ccacaccaga cacagccact gcccaccgtc gccgcgcctg ttttgagtgc accgctgggt
     8941 tctcacgaat ggcaacaatc attaagccag catatttcgc tgttcacccg ccaggggcaa
     9001 caaagtgcag agttgcgtct gcacccacaa gatttaggtg aagtgcaaat ttccctcaaa
     9061 gtggatgata accaggcgca aatccagatg gtttcaccgc atcagcacgt acgcgccgcc
     9121 ctggaagcag cgctgccggt actgcgtacg cagctggccg aaagtggcat tcagttaggg
     9181 caaagcaaca tcagtggcga aagctttagt ggtcagcagc aggccgcttc ccaacaacag
     9241 caaagccaac gcacagcaaa ccatgaacct ctggcggggg aagacgacga tacgcttccg
     9301 gttcccgtct ctttacaagg gcgtgtaaca ggcaacagcg gcgttgatat tttcgcctaa
     9361 cgtcagaggt agcaccgtaa tccgcgtctt ttccccgctt tgttgcgctc aagacgcagg
     9421 ataattagcc gataagcagt agcgacacag gaagaccgca acacatgact gattacgcga
     9481 taagcaagaa aagcaagcga tcgctttgga tcccgattct ggtattcatt accctcgcgg
     9541 cctgtgccag cgcaggttac agctactggc attcgcatca ggttgccgct gacgacaaag
     9601 cgcagcaacg cgtcgtgccc tccccggtct tctatgcgct ggataccttt acggtcaatc
     9661 tgggcgatgc ggatcgcgtg ctttatatcg gcataaccct gcgactgaaa gatgaagcaa
     9721 cccgctcgcg gctgagtgag tatttgccgg aagtccgtag tcgcttgctg ttactgtttt
     9781 cgcgtcagga tgctgccgta ctggcgacag aagaaggcaa gaaaaacctg attgccgaga
     9841 ttaaaaccac actttccacc ccgcttgttg ccgggcaacc gaaacaggat gtcaccgacg
     9901 tgctgtatac cgcttttatt ctgcgataac gacatgggcg atagtattct ttctcaagct
     9961 gaaattgatg cgctgttgaa tggtgacagc gaagtcaaag acgaaccgac agccagtgtt
    10021 agcggcgaaa gtgacattcg tccgtacgat ccgaataccc aacgacgggt tgtgcgcgaa
    10081 cgtttgcagg cgctggaaat cattaatgag cgctttgccc gccattttcg tatggggctg
    10141 ttcaacctgc tgcgccgtag cccggatatt accgtcgggg ccatccgcat tcagccgtac
    10201 catgaatttg cccgcaacct gccggtgccg accaacctga accttatcca tctgaaaccg
    10261 ctgcgcggca cagggctggt ggtgttctca ccgagtctgg tgtttatcgc tgtggataac
    10321 ctgtttggcg gcgatggacg cttcccgacc aaagtggaag gccgcgagtt tacccataca
    10381 gaacagcgcg tcatcaatcg catgttaaag ctggcgcttg aaggctatag cgacgcctgg
    10441 aaggcgatta atccgctgga agttgagtac gtgcgttcgg aaatgcaggt gaaatttacc
    10501 aatatcacca cctcgccgaa cgacattgtg gttaacacgc cgttccatgt ggagattggc
    10561 aacctgaccg gcgaatttaa tatctgcctg ccattcagca tgatcgagcc gctacgggaa
    10621 ttgttggtta acccgccgct ggaaaactcg cgtaatgaag atcagaactg gcgcgataac
    10681 ctggtgcgcc aggtgcagca ttcacagctg gaactggtgg ccaactttgc cgatatctcg
    10741 ctacgcctgt cgcagatttt aaaactgaaa cccggcgacg tcctgccgat agaaaaaccc
    10801 gatcgcatca tcgcccatgt tgacggcgtc ccggtgctga ccagtcagta cggcaccctc
    10861 aacggtcagt atgcgttacg gatagaacat ttgattaacc cgattttaaa ttctctgaac
    10921 gaggaacagc ccaaatgagt gacatgaata atccggccga tgacaacaac ggcgcaatgg
    10981 acgatctgtg ggctgaagcg ttgagcgaac aaaaatcaac cagtagcaaa agcgctgccg
    11041 acgcggtgtt ccagcaattt ggcggtggtg atgtcagcgg aacgttgcag gatatcgacc
    11101 tgattatgga tattccggtc aagctgaccg tcgagctggg ccgcacacga atgaccatca
    11161 aagagttatt gcgtctgacg caaggatcag tcgtggcgct ggacggtctg gcgggcgaac
    11221 cgctggatat tctgatcaac ggttatttaa ttgctcaggg cgaagttgtc gtagtcgctg
    11281 ataaatatgg cgtgcggatc accgatatca ttactccgtc tgagcggatg cgccgcctga
    11341 gccgttagtg atgaataacc acgctactgt gcaatcttcc gcgccggttt ctgctgcgcc
    11401 actgctgcag gtgagcggcg cactcatcgc cattattgcc ctgatcctcg ctgctgcctg
    11461 gctggtaaaa cggctgggat ttgcccctaa acgcactggc gttaacggtc tgaaaattag
    11521 cgccagtgct tcactgggcg cgcgtgaacg ggttgtggtg gtcgatgtgg aagatgcacg
    11581 gctggtgctc ggcgttaccg cagggcaaat caatctgctg cataaacttc ccccttcagc
    11641 accaacggaa gagataccgc agaccgattt tcagtcggtc atgaaaaact tgcttaagcg
    11701 tagcgggaga tcctgatgcg tcgtttgttg tctgtcgcac ctgtccttct ctggctggtt
    11761 acgcccctcg ccttcgcgca actgccgggt atcaacagcc agccgctgcc tggcggtgga
    11821 caaagctggt cgctcccggt gcaaacgctg gtgttcatca cctcgttgac gtttattccg
    11881 gcaattttac tgatgatgac cagtttcacc cgcatcatca ttgtttttgg tttattgcgt
    11941 aacgcgttgg gaacaccctc cgcgccacct aaccaggtat tgctgggact ggcgctgttt
    12001 ttgacctttt ttattatgtc gccggtgatc gataaaattt atgtcgatgc gtaccagcca
    12061 ttcagcgaag agaaaatatc aatgcaggag gcgctggaaa aaggggcgca gccgctgcgt
    12121 gagtttatgc tgcgtcagac ccgtgaggcg gatttagggt tgtttgccag actggcgaat
    12181 actggcccgt tgcagggacc tgaagctgtg ccgatgcgca ttttgctccc ggcctacgtg
    12241 accagcgagt tgaaaaccgc atttcagata ggcttcacga ttttcattcc ttttttgatt
    12301 atcgacctgg tgattgccag cgtgttgatg gcattgggga tgatgatggt tcccccagcc
    12361 acaattgctc tgccctttaa actgatgctg tttgtactgg tggatggctg gcaattgctg
    12421 gtcggttcgc tggcgcagag cttttacagc tagtgaggca aaatgacacc tgaatcggtc
    12481 atgatgatgg ggactgaagc gatgaaagtc gcgctggcac tggctgcccc actgttgctg
    12541 gtggcgctgg tcacgggtct tatcatcagt attttgcagg ccgccaccca gattaacgaa
    12601 atgacgctgt cgtttattcc gaaaatcatc gccgtattta tcgccattat tattgccgga
    12661 ccgtggatgc tcaatctgtt gctggattac gtccgcacct tgttcactaa cctgccgtat
    12721 atcatcgggt aggccgtaca atgatgcagg taacaagcga tcaatggctt tcctggttaa
    12781 gcctgtactt ctggccgtta ctgcgcgtgc tggcgctcat ttctaccgca ccaattctga
    12841 gcgaacgcag cgtgccgaaa cgggtaaaac taggtctggc aatgatgatc actttcgcta
    12901 ttgccccatc attacctgcc aacgatgttc ctgttttttc gttctttgct ctgtggctgg
    12961 ccgtgcagca gatcctgatc ggcattgcgc ttggttttac catgcaattt gcctttgccg
    13021 ctgtgcgaac cgctggtgaa attatcggtc tgcaaatggg gctgtcattt gcgacgtttg
    13081 tcgatccggc cagccatctc aatatgcccg ttttagcgcg tatcatggat atgctggcgt
    13141 tactgctgtt cctgacattt aacggtcatt tatggttgat ttcactgctg gtcgatacct
    13201 ttcacaccct gccaattggt ggcgaaccgt tgaacagcaa tgcgtttctg gcactcacca
    13261 aagcagggag tttgattttc cttaacgggc tgatgctggc attaccgctc attactctgc
    13321 tgctgaccct gaatctggca ttaggcttac ttaatcgtat ggccccgcaa ttatccattt
    13381 ttgttattgg atttccatta actctgactg tcggtatctc tttaatggcg gcattaatgc
    13441 cgttaattgc gcctttttgc gaacatttat tcagtgagat gtttaatttg ctggctgata
    13501 ttattagtga attgccatta atataattcc gtaacgttta tcatgttatc ctaaggatta
    13561 tccgaaaaat aatacctacg aacatcttcc aggatactcc tgcggcgaaa tatttgtttt
    13621 aagctcactc acatatcgca acatttactt tactttaaga caattccagg caaattatac
    13681 aacactttac gggatagtaa gtccgcctga aaaatcgcga gagtggcgca ttaggtgacc
    13741 catgttgttc cgtttagtca tgatgaaata ttcaggtaag gggaattatc gttacgcatt
    13801 gagtgagggt atgccatgtc aacgattatt atgaatttat gtagttacac ccgactaggt
    13861 ttaaccgggt atctgttgag tagaggggtt aaaaaaagag aaatcaacga cattgaaacc
    13921 gttgatgacc ttgccatagc ttgtgattca cagcgccctt cagtggtgtt tattaatgag
    13981 gactgtttca tccacgatgc ttctaacagt cagcgtatca agctcatcat taatcaacat
    14041 cccaatacgt tatttatcgt ttttatggca attgccaatg ttcattttga tgaatatcta
    14101 ttggtcagaa aaaatttatt gatcagttct aaatcgatta aaccggaatc tctcgacgat
    14161 atccttggcg atattctgaa aaaagagaca acgataacct cgtttttaaa tatgccgacg
    14221 ttatcattga gccgaaccga atcgagtatg ttgcgaatgt ggatggcagg tcagggaacc
    14281 attcaaatct ctgaccaaat gaatatcaaa gccaagaccg tttcatcgca taaaggtaat
    14341 attaaacgta agatcaaaac gcataataaa caggttatct accatgtcgt ccgactgacg
    14401 gataatgtga ctaatggtat ttttgtcaac atgcgctaac acattctgac tggtggtttc
    14461 ccaccagtca ggctgaatta gattactctg ctttctccac aaagataccg tcctgatgcc
    14521 ctgcttcatt aaagaaccag atgccgagcg ggtagtcttc cagcgaaacc aggtacattg
    14581 tgccttcact aaactcctca actgccagta ccacgccagg acgacgcgga ccgccatccg
    14641 ttttgactgt tacccgatca ttcaccttca tcgttttcct cctgtggctt tgtgccagtg
    14701 tagaacaatt tcgtttttct ggcagcgcca ggcgcgcgtg agtgctgatt ttctcgacgg
    14761 tctatactta agagatgcca ggcggactta acgactggcg gcaacaacag agtaacggtt
    14821 gcgaggaaag atgatgaaaa ccgcaaaaga gtacagcgat accgcaaaac gtgaggtcag
    14881 cgtcgatgtc gatgccctgc tggcggcgat caatgaaatt agcgaaagcg aagttcatcg
    14941 cagccagaac gattctgaac acgttagcgt cgatggacgt gaatatcata catggcgtga
    15001 attggcggat gccttcgaac tggatattca tgacttcagc gtctctgaag tgaatcgttg
    15061 aatgcacaat aaaaaaatcc cgaccctgag ggggtcggga tgaaacttgc ttaagcaaga
    15121 agcacttaaa aaattcgtta caccaggaaa tctgatgtgt tcatcacctt atccgcaatt
    15181 tttttcgctg acaagaaaat attcgtgata tgaatgatta acgctactta tgaatagaaa
    15241 tgtgacgcgc gtcacccctt ggtggctctg gcgtcaacgg gatcgctccc gaaaaaaaca
    15301 tctttgataa tttgtgctgt tttgagaatc atcctggtaa ggggagtact gcgcaaccat
    15361 gctttcaatt caacaaccac tactggtttt tagcgatctt gatggcaccc tgctggacag
    15421 tcatagttat gactggcaac cagctgcccc ctggctcagc cgtttacgcg aagcgaatgt
    15481 tgccgtcatt ctctgtagca gtaagacatc agcggaaatg ctgtacttgc aaaaaacgtt
    15541 ggggctacaa ggtttaccgc tgattgccga aaatggcgca gtgatccagc ttgctgagca
    15601 atggcaggat atagacggtt ttccacgcat catctcaggt attagccatg gcgaaatcag
    15661 ccaggtttta aatacgctac gcgagaaaga acattttaaa ttcacaactt ttgatgatgt
    15721 cgacgatgca accatcgccg aatggacggg attaagccgt agccaggcgg cgctgacgca
    15781 gctgcatgag gcgtcggtaa cgctaatctg gcgcgacagt gacgagcgta tggcacaatt
    15841 taccgcgcgt ctgaacgaac tgggcttgca gtttatgcag ggtgcgcgct tctggcacgt
    15901 cctggatgcc tctgccggaa aagatcaggc tgccaactgg attatcgcga cctatcaaca
    15961 attgtcaggc aaacgcccaa ccacacttgg cctgggcgat gggccaaacg atgcgccctt
    16021 actggaggta atggattacg cggtgattgt gaaagggtta aaccgtgaag gggtgcatct
    16081 gcatgatgag gatccggccc gcgtctggcg aacgcagcgt gaaggaccgg aaggctggcg
    16141 tgaagggctg gaccattttt tctccgccaa ttaagcgtta tcgctcgcga atacccgatt
    16201 gcgcccggcc tgtttagcga gataaagtcg acggtcggcc agtgactgga gttgttcaaa
    16261 atcataatca ccggtttcct cgctgctgct tacccccagc gaggcactga tgcgtatcgt
    16321 cgtactcttg gcgatcaaca tctctttttc atttagcttc aggcgaatac gttctgcaac
    16381 ctccgcagcc tgcgtcaggc tcgcgccagg cagaatcaca caaaactcct caccaccgac
    16441 ccgcccggca acgtcctgcg cacgcaagga actgctaatt aatccggcag catgagaaag
    16501 aacacggtcg cccgcctgat gaccaaagcg gtcattaatc gctttaaaat ggtcaaggtc
    16561 gacctgaatg acagaaaaag gatgttggtg tgtctgacac aatttggcga gcggacgggc
    16621 tttttcaaac agtgcgccac gattatataa gcgggttaag gtgtcgtgcc acgcctgcca
    16681 ctgcaacgaa ctttgcagaa catacatgtt gctgaccatc cggcgaatca cataccagga
    16741 gatgagtaac atggtggtaa agagcgccca cagcagggtt aatgcaatgc tgatactgcc
    16801 gaaatcgccc cgcacgcctt cgcttagcgt atggacgcgc accagcacac cgtcgaaatg
    16861 gtccagacgt tcccagctaa catagcgact gttcatacga atgccgcccc gcgtgtcatg
    16921 ttccattgcc tgcgccagca aggccagttc acgaggatca aaaatattcc ctgttggatg
    16981 gtcaggattg gaagaggtca aaaatctcag cttactgtca tagagctgat actcaccatc
    17041 gaggttttta tcgatggcgt ttcttaaaaa ttgctgcatg gtacgcacgg gaatactcat
    17101 ccccagcacg ccataccagt agttattact gtctaccgga acactgacgg taacctgcgg
    17161 ttcagtattg ctggcgtgtt ccggttgcga agtgaaccag cgtaccgcgc ggtgacgatt
    17221 ttctcgttgt gaatggccga taaaccaggg ttgggtgaca tagccgtaat aacgcgttgg
    17281 tacattgcgc gtaaataagg tcggctgcgt cgaaacgtaa aatccggcac gcgagacata
    17341 catcgcctgt tcaaccatcg acgaggagtt gtgcgccagt cgcagcaagt aaccaacttc
    17401 cagtgcagcg gtaatttcat tgtcgaggct ttcattttcg cgagacagga gattcccctc
    17461 gctgactaat gcacccgaca cgccattgac tggcagggtg cgtcgtcggt tgagttcgat
    17521 ttgccaggcg tgctcgtcgc gatgctgttc gaactcggtt acggcattac gcagagaggt
    17581 gaaatccagt ggcgctacga gagcttcgcg catgccatta cgcaaaaaga tcagtttgtc
    17641 gacgttatac tgcaaatgct tatcgagcgc gttggctacg ttttccagat gattacgctg
    17701 gctggagata taggcatctt ccagcactac cacttcgcgc caggtcagca aggtggaaaa
    17761 aagcaatacc acgataaagc agagattaac cacatgacca ggccccaggc ggcgtgcgag
    17821 ttttttcaac cagctctggt tttccatttt tgtctcgtgc tgcaccctga cgactcccaa
    17881 cctgcttttt tatgattctg gcataacccg gcgataacgt accgcaaaac agcgataatt
    17941 ttataatggt ggcatgataa gacgcgacta tcattcattg caggcattgg cacaccaggc
    18001 cggatgtgat ccggcctggt gttaatgata ccctttcaaa cttcttccgc agaacgactc
    18061 ccctcccccg gaacaagctc ggtttcatga aaagcttccc gcttgacccc gtaaccgtca
    18121 taccaacgac actcaaccat accgctggag tatccagtga caatcatccg cgggccgccc
    18181 tctttaaccg taacttcctc actaaccata aagctcatac tcgcctcctt ttttcgagtg
    18241 aaacttgttc accttagttg aagatggcga attttgcata atagcctgtg cgagtattaa
    18301 tgtgcctgac cgcgcatttt cgctacggct ttcactccca gcaccacgat gccgccgatg
    18361 ataaatccaa gaatcagatt taaaacagtc ggtaatatca tcgccaccac tgcactttgc
    18421 tgcccggcga aatgttcaat ggcgtgatgc aacggcgcaa taccatgtac cacaatcccg
    18481 ccgccgacga ggaacatcgc cagcgtgccg acaatcgata atgctttcat cagccagggc
    18541 gcgataatca ataacccttt acctaatgcc tgcatcagcg cgctggattt ttccgccagc
    18601 caatacccca ggtcatcaat cttaacgata acccctacca gaccgtagac gcccacggtc
    18661 actaccagcg cgatgcctga aagcaccagc acctgattaa gcaacggtgc ctctgccaca
    18721 atccccagcg tgatggcgac gatttccgca gacaatataa aatcggtacg aatcgcccct
    18781 tttattttgt ctttttcaaa cttcagcgga tcctgcgccg ccagcttctc cagacgctgc
    18841 tgactctgcg ccggatcttc tttatgttta cgcgcctcca gcatatgcag cactttctct
    18901 accccttcaa agcagagaaa cgcgccacca atcatcaaca gtggcgtaat cgcccacggg
    18961 ataaacgcac tgatgatcag cgccagcggc accagaatca ctttattgat cagcgatcct
    19021 ttcgccacgc cccagaccac gggaagttcc cggttggccc gcacgcctga aacttgttgc
    19081 gcattgagcg ataagtcatc ccctaataca ccggcggttt tcttcgccgc cagtttgccc
    19141 atcacggaga tatcgtccag cagtgtggcg atatcatcga gcaacgtcag taaactactc
    19201 ccggccaaaa gcattccctc tcatttttat tgttgaaggg tgtaagtatg aagcaaaaac
    19261 ggcagctctg atacttgctt cacaggtcaa catttattta acaatcaagt gcaattatat
    19321 ctctcgccag cagaatgatt ttcacgttta ctatgaacca cctttttatt tccaccgtga
    19381 gggattatgc gtttccgcca gttgttaccg ctttttggcg cgctgtttgc gttgtatatc
    19441 atttggggct caacctattt tgtcattcgg attggcgtgg aaagctggcc tccattaatg
    19501 atggcgggcg ttcgattcct ggcagccggt attttattgc tggcattttt gctactgcgc
    19561 ggacacaagc tccccccgct acgtccgctg ctcaatgccg cgctgattgg cctgttattg
    19621 ctggctgtcg gtaatggcat ggtgacggtt gccgaacatc aaaatgttcc ttccggcatc
    19681 gccgccgttg tggttgcaac cgtgcccctc tttaccctgt gcttcagccg cctgtttggc
    19741 attaaaacgc gcaaactgga atgggtgggt atcgccattg ggcttgccgg aatcatcatg
    19801 ctcaatagcg gtggaaattt aagcggcaat ccgtggggcg cgattctgat tttaatcggt
    19861 tcgattagct gggcgtttgg ctcggtttat ggctcgcgca ttaccttacc tgtagggatg
    19921 atggcgggtg cgattgagat gctggcagca ggcgtggtgt taatgattgc gtcgatgatt
    19981 gcgggtgaaa aactgacggc gctcccttcc ctttcaggct tctttgcggt cggctatctg
    20041 gcgctgtttg gttcgattat cgctattaac gcttatatgt atttaatccg caacgtcagt
    20101 ccggctctcg ccaccagcta cgcctacgtt aacccagtgg tcgcggtctt gctgggtacg
    20161 ggactgggcg gcgaaacgct gtcgaagatt gaatggctgg cgctcggcgt aattgttttc
    20221 gcggtggtac tggtcacgtt gggaaaatat ctcttcccgg ccaaacccgt tgtcgcgcca
    20281 gttattcagg atgcatcaag cgagtaaatg aatcccctgc gtgtcgatct gcgcgctggc
    20341 accttcgccg cagatccact cttccagacg ctcggtaagc gccgcatccg tcagcttctc
    20401 gcgcccacgt aacgcacact cccagacaat cagtacgcgc cagccgagtt cctgcaagcg
    20461 actgatatcg cggcgatcgc gttcaacatt tttacctatc ttctccagcc agaactcggt
    20521 tcgagtcgca ggcactttaa acagatagca gtgatgatga tgccagaagc agccgtgggt
    20581 aaatatcacg cagcgatatt catcaacgac aaaatccggg cgtccgggga gatccgcgtc
    20641 ctgaacgcga aacgccaggc cctgcccggt taacaaactg gcgaggcgct tctcaatcgc
    20701 cgtatcacgc gtagcaatcg cgcgcatatt tttgctgcga gtggccttat cgtgaacgtc
    20761 gaccatgttg tgcctcttgc tgacgcaacg ccaccgcctg tttgattttt ggctcaagca
    20821 gttttgccac cgcggcaaag accggcacga ccaccgagtt accaaactgg cgataagcct
    20881 gagtgtccga aaccggaatg cggaatttcg cttctcccgg cgcttcaaaa cccattaagc
    20941 gcgcgcattc acgaggcgtt aaccgacgtg gacgatgttg ctgattcagc ggatcgtcaa
    21001 agtctttttc acccgtggcc atatcccagc cgcgatcgat taaaatttcc gcgccatctt
    21061 tgtaataacg cgcagacaac gtacgcgtga cgctttgcgg attgttcgga taaaccattc
    21121 cataaccgaa gccattacca cgcgcctgat gttttttcgc ataacgatag aggtacttcc
    21181 acagcaccgg cgtcaggata tatttcgcct cgaccatcgg gtccagcagc tgggccagcg
    21241 tcactcgctg cgcagggaaa catttgctga tatcacgcag ggtaaaatcg gctttcagat
    21301 tcagatcgcg acgaaaaccc accagcacga tgcgttcacg gtgctgcggc aggaaatgtt
    21361 tgccatcgat gattttcgga tcgtccggcc cgttatcttc tgcatcagcc acgtcatagc
    21421 ccagttcgtc cagcgtctgc atgatgatgc ggaacgtttt accctggtcg tgacttttca
    21481 ggtttttgac gttttcgagc acaaacatcg ccggacgacg cgcgtcgata atgcgcacca
    21541 catcaaagaa cagcgtgccc tgggtatcgc aggcaaaacc gtgcgcccgc ccgagcgagt
    21601 tctttttcga tacgccagcc agcgaaaatg gctgacaagg aaaacccgcc agtaatacat
    21661 cgtgttcagg aatgtgttga cgaatatgtt ccgccgccgc ctcatcactc acgccttctt
    21721 tatggctgag ggtgatgtcg cggatatctt cattaaaatg atgcgtcgcc gggtcgcaat
    21781 aatgattggc tttataagtg cgtaccgcat gtttgttcca ttcgctggta aacacgcact
    21841 gtccgccaat cgattcaaaa ccgcgacgga tgccgccaat tccggcaaat agatcgataa
    21901 agcgaaacgc ataatgcgga tgatgtgccg gtggtttggg taacaacgtt tgcagatggg
    21961 cgaactcttt ctcacttaaa cggtgccata ccgagtcatt cgccagcgca cgttttaaaa
    22021 ttgccgcgct ccagtgattc tcacccacac cattaagctg cgccaccagc gttttaacat
    22081 cataaatttg cagcagtttc tccagcattg cctgtgcggc attcccggtg ctgtatgaat
    22141 cggttactga tatattttcc tgcatagatt taaccggcca tctgagatgg ccgacaggtt
    22201 aacataatta cagccattta gggccacgcg ctcagcccgc gggtgaaaac gcatctatca
    22261 ccttgtgatc gacaccttca ttctcccccg ccagttcggc actgagcttc gccataaact
    22321 cgactaaaaa ttgcgcgttg tgctgcgcca gctgcttgcc tcttgcggtt tgcatggttt
    22381 gcggtagctt cagcaactta gtctggaaat gatccagcgc gtagcgttta tcatcaagcg
    22441 gacgatgctg tgcgaacgga tcttcaccat caaacagcgc cacgcccaat gcccctgaaa
    22501 cggcaaacac gcgcgccagg ccaatcgccc ccagggcttc cagccgatcc gcatcctgga
    22561 cgattttagc ttccgttgtt aagggggcga tttgcgcgct gaaactgtga gcggcaatgg
    22621 catgacaaac ggcctcgatt ttctccgccg gaaattgcac gaactcttca cggagcaggc
    22681 gacgcgtctc ttctgccgcc aggattgaag aacgctgccg ttgtggatga tctttcgcca
    22741 ggctcacaat atcgtgaaaa taacaggcgg ttaaaatcac cagcatatcg acatcgtcat
    22801 ctgccgccag cttctgtgcc gtagcccaga cgcggcgaaa atgacatacg tcatgggccg
    22861 cgtcctggtg ctgatgatgg tttttcaacc agttttcgaa ctgtgcctgc cagtgttgta
    22921 agtccatacg cgctccgttg ccaaagagtc gcaaccttag cagtttttat tcatagcgtc
    22981 tgctacgtgc gggacgaggt cgggtgtacc aggcaatgct gcctaacaat gccccaacca
    23041 gagccataaa taaaaagccg accaatgctc ctaaccactt ccccgcagta gatcctaaat
    23101 cactttcaaa tcccgacacg ggggcatcgg ggatccggct catcacccag atgtaacgcg
    23161 ccattgccca gacgaaatag ccacaccatg tatagaaagc ccacaaagcc agtttgccac
    23221 caggactgcg agagagtctc tcttccatct ttgtcgaacc gtattccatt ttgagataac
    23281 ccggaaaatt cagacttagt actattatgt gataaaagtc acatttttcc acattggatg
    23341 agccagtcaa gactcaatca ttttttgaac aaatagataa tttttgcgca acggcagtga
    23401 atacaggaaa aatcgttttg agcgatttta tttatctttg atatcggctt tattaattca
    23461 tttaatcaat atattagcac tgattacaat tatacctttt tattattctg tgtcatgatg
    23521 cttccgttat tagcctttta tcgtcttgtt tatatttttt ggggcggcat gatgccggct
    23581 ttttttatgc cttcattaat gtgcgcgaga tcacgccagc cgtttagcgc aacaatcatt
    23641 gacactccct atcttttcaa tcaactcata aca
//
LOCUS       DAZFFE010000073        23647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFFE010000073 DAZFFE010000000
VERSION     DAZFFE010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.073"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1454)
                     /gene="cadC"
                     /locus_tag="ACHJ69_003092"
     CDS             complement(<1..1454)
                     /gene="cadC"
                     /locus_tag="ACHJ69_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO5016027.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTTTPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSNLETRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLQSLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLDRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANT"
     gene            complement(1714..1825)
                     /locus_tag="ACHJ69_003093"
                     /pseudo
     CDS             complement(1714..1825)
                     /locus_tag="ACHJ69_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2071..2146)
                     /locus_tag="ACHJ69_003094"
     tRNA            complement(2071..2146)
                     /locus_tag="ACHJ69_003094"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2111..2113),aa:Phe,seq:gaa)
     gene            complement(2253..2828)
                     /gene="yjdC"
                     /locus_tag="ACHJ69_003095"
     CDS             complement(2253..2828)
                     /gene="yjdC"
                     /locus_tag="ACHJ69_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5016028.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(2865..4562)
                     /gene="dsbD"
                     /locus_tag="ACHJ69_003096"
     CDS             complement(2865..4562)
                     /gene="dsbD"
                     /locus_tag="ACHJ69_003096"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO5016029.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNEASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGETHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTVNDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(4538..4876)
                     /gene="cutA"
                     /locus_tag="ACHJ69_003097"
     CDS             complement(4538..4876)
                     /gene="cutA"
                     /locus_tag="ACHJ69_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO5016030.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(4992..6293)
                     /gene="dcuA"
                     /locus_tag="ACHJ69_003098"
     CDS             complement(4992..6293)
                     /gene="dcuA"
                     /locus_tag="ACHJ69_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO5016031.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(6411..7847)
                     /gene="aspA"
                     /locus_tag="ACHJ69_003099"
     CDS             complement(6411..7847)
                     /gene="aspA"
                     /locus_tag="ACHJ69_003099"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO5016032.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            8184..8660
                     /gene="fxsA"
                     /locus_tag="ACHJ69_003100"
     CDS             8184..8660
                     /gene="fxsA"
                     /locus_tag="ACHJ69_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO5016033.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            complement(8676..9932)
                     /gene="yjeH"
                     /locus_tag="ACHJ69_003101"
     CDS             complement(8676..9932)
                     /gene="yjeH"
                     /locus_tag="ACHJ69_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO5016034.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSGQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPGNGITT"
     gene            10208..10501
                     /gene="groS"
                     /locus_tag="ACHJ69_003102"
     CDS             10208..10501
                     /gene="groS"
                     /locus_tag="ACHJ69_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO5016035.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            10545..12191
                     /gene="groL"
                     /locus_tag="ACHJ69_003103"
     CDS             10545..12191
                     /gene="groL"
                     /locus_tag="ACHJ69_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO5016036.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            12329..12682
                     /gene="yjeI"
                     /locus_tag="ACHJ69_003104"
     CDS             12329..12682
                     /gene="yjeI"
                     /locus_tag="ACHJ69_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO5016037.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(12732..13601)
                     /gene="yjeJ"
                     /locus_tag="ACHJ69_003105"
     CDS             complement(12732..13601)
                     /gene="yjeJ"
                     /locus_tag="ACHJ69_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO5016038.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSAMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDDQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGNEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(13836..14864)
                     /gene="epmB"
                     /locus_tag="ACHJ69_003106"
     CDS             complement(13836..14864)
                     /gene="epmB"
                     /locus_tag="ACHJ69_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO5016039.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            14906..15472
                     /gene="efp"
                     /locus_tag="ACHJ69_003107"
     CDS             14906..15472
                     /gene="efp"
                     /locus_tag="ACHJ69_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO5016040.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            15524..15649
                     /gene="ecnA"
                     /locus_tag="ACHJ69_003108"
     CDS             15524..15649
                     /gene="ecnA"
                     /locus_tag="ACHJ69_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO5016041.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            15760..15906
                     /gene="ecnB"
                     /locus_tag="ACHJ69_003109"
     CDS             15760..15906
                     /gene="ecnB"
                     /locus_tag="ACHJ69_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO5016042.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            16082..16399
                     /gene="sugE"
                     /locus_tag="ACHJ69_003110"
     CDS             16082..16399
                     /gene="sugE"
                     /locus_tag="ACHJ69_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO5016043.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(16396..16929)
                     /gene="blc"
                     /locus_tag="ACHJ69_003111"
     CDS             complement(16396..16929)
                     /gene="blc"
                     /locus_tag="ACHJ69_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO5016044.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(17018..18151)
                     /gene="ampC"
                     /locus_tag="ACHJ69_003112"
     CDS             complement(17018..18151)
                     /gene="ampC"
                     /locus_tag="ACHJ69_003112"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO5016045.1"
                     /translation="MLKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(18214..18573)
                     /gene="frdD"
                     /locus_tag="ACHJ69_003113"
     CDS             complement(18214..18573)
                     /gene="frdD"
                     /locus_tag="ACHJ69_003113"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO5016046.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(18584..18979)
                     /gene="frdC"
                     /locus_tag="ACHJ69_003114"
     CDS             complement(18584..18979)
                     /gene="frdC"
                     /locus_tag="ACHJ69_003114"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO5016047.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIMIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(18990..19724)
                     /gene="frdB"
                     /locus_tag="ACHJ69_003115"
     CDS             complement(18990..19724)
                     /gene="frdB"
                     /locus_tag="ACHJ69_003115"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO5016048.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(19717..21525)
                     /gene="frdA"
                     /locus_tag="ACHJ69_003116"
     CDS             complement(19717..21525)
                     /gene="frdA"
                     /locus_tag="ACHJ69_003116"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO5016049.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            21850..22827
                     /gene="epmA"
                     /locus_tag="ACHJ69_003117"
     CDS             21850..22827
                     /gene="epmA"
                     /locus_tag="ACHJ69_003117"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO5016050.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            23046..>23647
                     /locus_tag="ACHJ69_003118"
     CDS             23046..>23647
                     /locus_tag="ACHJ69_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5016051.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNP"
BASE COUNT         5863 a         5804 c         6020 g         5960 t
ORIGIN      
        1 gtgtttgccc ctgggcgtaa attaaaggcg gtgagatatg catcagctgc ttcccggttc
       61 atccccttca tttcataaac cttgccaagc aacacataat ttagccagga catttcaaga
      121 tcaatgccgg tatttatcgc ctgataagac tcatctgttt taccttttac cagggcactg
      181 accgctttta tttgatatat aatggacagg ttgttcaatt ccggcagtgt aacaatgtta
      241 tctatttctg tgttcagtgc tgctaattgt ttttcgtcta aaggatgttg agaatggcgc
      301 acgatatcaa ctaatgcttt ttctgctctc gcgtaggtaa attctgggga tgattgaaca
      361 atctcaccta ataattcact ggcacgatcc aatgatttat catcaccatg cagtaaataa
      421 tcatgtgcct gataaaaatt agttaataac gcaccacgat gcggcaaaat ttgctggagc
      481 atctcctgca ttcgttgtgg ccacggttgg tttaacgctt ttgataaact ctggagcaaa
      541 tcattttgaa tcgccagctg attaccgtta gtgatgacat aacgtttatc cagcatggtt
      601 gaaccatctg cattgtctac caattttacc gacataaagc attgttgagc acggtattgg
      661 cgctgattaa caaacgcaat agataatgtt ttaccggaac tgctcggttc atcaatgttg
      721 tagttgattt tgtcatgcac cataaaggtg gagaaggtgt taagtgatgt cgccaccaaa
      781 tcacccacgc ctatcgcgta agagagctga tacggagaac tccagctgtt acaactctta
      841 ttaaccatat taatgtcaat atcgcgtgga ttgagcaaaa tgcgcgattt actcatagga
      901 agacgtgttt caagatttga aaacgctacc agtgcgacac agatacctaa cgacaacagg
      961 aaaaaaaacc atacccaaaa ggtagtgaat cgtttgcttt taactgggga ttgttcaggt
     1021 ggcgttgtgg tgttttgaat gttaagactg tgggagggag aatctgtggc aggaaccgcc
     1081 tctggtatag ggggaggcga agatagcatt atttcctctc cctcttcttc gctgtaccag
     1141 ataaccggca ccattaattt atagccgcgc tttggtacag tagcgatata gacaggacta
     1201 tcttcatcat tatcttttaa tgacttacgt agttctgaga tactctgcgt cacaacgtga
     1261 ttggtgacaa tacttctctt ccagacatta tcgataagtt catccctgct aagtacttcg
     1321 ccactgtgtt gagcaaagaa aaccagaaga tcgattaatc tcggctcaag ggtaagttga
     1381 cgcccattgc ggctaatttg gtttatggac ggagtaacaa gccattcgcc aacgcgaact
     1441 acaggttgtt gcataataga aactcattcg aaaagggaat gatgcaatga taattgccat
     1501 aacctatttt ttccatctat agatgggttt atttacatat tattggtgaa tgtaggacgt
     1561 tatttttacc cgccataaac ttcttgatta catagtatta cgaaaggatt ttactgagaa
     1621 ccagaagtaa ttttccttac catcaaaatt catcatcttt gccaaagaaa aatgttcaga
     1681 aaataatcca tgaaaaattg tccggagcgc ttactatttt aatggattgt tagtctttgc
     1741 atgagcaaac ggactgatac atttctcttt gttctcattc agaaaatctc atcagttggc
     1801 gttctgcccg atgttgtgct ttatgggtgc gttacgctcc tgatgacgtc atttgacgtt
     1861 caacagcatc acggggccgc acgacatttc acgtcagtca gcgctatagc tcagaaacaa
     1921 attttcccga attgggatat gcccgcaaat tgctggtgat gtggggagaa tctggttgag
     1981 ttcggtagaa ttgatttggc ggcaggacgc ttaaatcgtg gcgtcctgaa acgaaaaacg
     2041 gacctccgtg gaggtccgtt tatatgaatt tggtgcccgg actcggaatc gaaccaagga
     2101 cacggggatt ttcaatcccc tgctctaccg actgagctat ccgggcaacg gggcgcatta
     2161 aaccgtaatc tgcacatctc gtcaacctaa tttcagtaaa agcgattcaa ctgcttaaga
     2221 ttgcggcaaa tcgcttcttt tctgtgtttc tgtcaggtca gtgcaccacc ctgacggcag
     2281 cgggcgaagc gcaggatatc ttccgccagc cgatgtgcgg tgtcgacatc cgcctggcta
     2341 cgattcacca gcattcggct taaacagcct tccagcacca gttccatctg ctttgctacc
     2401 atcgccggat cgtcaacttc cagcgtggtt aacagttcgt gggtgaaatc gtaggccgcg
     2461 cttttttgct gatcggccag ttgatgaata gggtggccag gatcggggta aaacgtacag
     2521 gcagcgataa acagacagcc cggatagcgg ttgtttttaa cgcactccga taacgcctga
     2581 taacgtgcca gcaacttttg ttcggcggtt tgcgtttcgt ccagcatcag ctgacgacgc
     2641 cagacatcta tctgttggct aagataacgc agcgcatcgt agaggattgc ctctttgtct
     2701 ggccagaagc ggcgtagctc gtccagtgga taatccacac gttcagcaac catctccagc
     2761 gtggtgttgg caatcccttg taattctaat aatttcaggg cttctcccag tacatcttca
     2821 cgttgcacgc tattttcctc cgtctttccc actgcaagtg tcgttcacgg ttggcgatcg
     2881 cgcaaatgtg cgctgaaggt ttcagcatcc ataaagcccg tgacgcgtgc ttgtggatgc
     2941 tcctggcctt gtccgtcaaa aaagagaatt gtcggtaggc caaggacatt aagatgcttt
     3001 aacagcgcca catcttgtgc gtcgttgaca gtgacgttgg cctgaagtaa gaccgtatct
     3061 gctaacgctt tttgcacctg cgggtcgctg aaggtgtatt tctcaaactc tttacaggcg
     3121 acgcaccagt cggcatagag atctaacatc actggtttgc ctttggcttc aacgagcgcc
     3181 tgatttaact catctactgt tttgatttgt gtaaagttga gatgcgtctg agtttgcgcg
     3241 gtatgtgttt caccaaatgc ccaatcctga agtgggcgca cgctaaccaa tgccgctgcc
     3301 agcaggatta tttgcaccac gcgcatccag ccgcgtttgg cctgtaggct ggtgataaag
     3361 gcccagccaa agaatgcgac accaagcgcc gaccacaagc gtaatcccca tatatcacca
     3421 atcactcgct ccagcaggaa gaccggcagt gcgaggatca caaaaccaaa cgcggttttg
     3481 acttgttcca tccacgggcc gcttttcggc agcaagcggt taccaaagac ggtaattagc
     3541 atcagcggca ggcccatgcc cagcgcataa agataaagcg tgccgccgcc cagccacatg
     3601 ttcccgcttt gggcgatata cagcagaatc gcgctaagcg gtgcagtggt gcatggtgaa
     3661 cagatcagtc cggcaatcgc ccccataata aacacaccgc caggtgagcc gccctgttgg
     3721 cgattgctca tcagcgtgag acgtgtttgc agcgaagagg ggagttgcag agtaaacaag
     3781 ccaaacattg acatcgccag caaggtaaag acgatggcga ggccaatgag cacgtatggg
     3841 tgctgtagcg ccgcctggaa ctgtaaccct gcggcggcaa ccaccagacc cagcgccgtg
     3901 taagtcagcg ccatcccctg cacataaata aaggtcagca acaatgctct ggcagtggaa
     3961 agccgctgtt taccgcccag cacgatgcca gaaatcagtg ggtacattgg cagcacgcat
     4021 ggcgtaaagg cgataccaat accgatcaac aacgcccaga gcgcggaaaa gggcaattgc
     4081 gcggtgggct gctcttgctg cggaacagac acaggctgtg acgcttcgtt gttagcgacc
     4141 acttcgctta acggaacggt tttggtttct ggcggataac agaaaccggc atcagcacag
     4201 ccctggtagg tgacagttaa cgttgctccc gcactcgcct ggttgatggt tacgggaagc
     4261 gtcagccgat cgcggtaaat ctcgcttttg ccgtaaaact catcttcatg ccagacgcct
     4321 tgcggcagct gcacgtcagc aattttcgcg tgttccggcg taatgcggat ctgtttacgg
     4381 tagaggtagt aaccgtcttt gatctgccag gtcagattca gatcatgttg attttgctga
     4441 aaatcaaaag caaaggcttg atccgcgggg acaaattgtg aacgtcccgg cgcgtcgaat
     4501 aatccggcaa aaacggaagt gctgcaaagt agcaggatca gcgtaaagat gcgttgagcc
     4561 atgagaggta atctgtgtct ccgtgtgtaa caggtaaaac cagaagttcc ggggtttgat
     4621 atggatgatg agacttcagg cattccagca gtgcctgctg gtgagatacg gtagttttta
     4681 aaatcatctg cacttcgtat tcttgctcca gcttaccttc ccagtaatag agagaggtag
     4741 cgccggggat caaggtcgcg caggccgcca gtttttccgc cagcactttg gcggctaaat
     4801 cctgggctgt cgcttcatcc ggtgccgtac atagcaccac gacagacgtg gtattcgaac
     4861 ttttttcatc aagcataaac acctcgcaag aacagatgga accgcaagag agaggtcact
     4921 atacaacgga cggggaaagg atgttagtcg gcgggaaaga aagcggggcg tgaacgcccc
     4981 gcgatgggtc attacagcat gaagctaccc agcacgaagc cgaagcaaac ggccagggca
     5041 acacccagag tacccgggat gaagaacgga tggttgaaga cgaatttacc gatacgggta
     5101 gtacccgtat catccatctg taccgcagca accagcgtcg ggtaggtcgg cagaatgaac
     5161 agaccagaca ccgcagcaaa agaagcaaca gcggtcagcg gagaaacgtt cagtgccaga
     5221 gccatcggca tcagtgcttt tgcggttgca gcctgagagt acagcagagc agaagcaaag
     5281 aagaagatga cggccagcag ccacggatga ccctgaatca cttcaccagc ggtatctttg
     5341 atccagtcga tgttgttgga aacgaaagta tcgcccagcc acgcaacacc caggatacaa
     5401 atacaggcgc tcatacctgc tttgaaggtg ctggagttga gaatgttgtc ggtatcgact
     5461 ttacagataa cggtggtcag agttgcaacg ctgagcatga tgatcaggat tgcgttggtg
     5521 gtgttcatca gtggtttttc aaccagaccc atgcttgggc tgttgatgat tgcatagata
     5581 accacgccaa ctacgcccag caggaacagc cagacggacg tttttgcacc ggatttgatt
     5641 tcaatctgct tttcaccgcg cagttcaacc aggccctctt ccagacgctt gcgataaatc
     5701 ggatcgtcag agagtttgga gttgaacagc atagtgacca ggaaggacat caccagaacc
     5761 gccagcaggg tggacgggat gaccacggag agcagatgga ggtagctgat gccatgacct
     5821 tccatcacgg aagacatgta aaccactgcc gctgagattg gcgatgcggt gatcgcaatc
     5881 tgcgcggata ccactgcagt agacagcgga cggcaaggtt taacgccttg ttccttcgca
     5941 acttcagcga taactggcag tgtcgccaga gagatgttgc cagtaccagc aaagatagtc
     6001 aggaaatagg tcacgatcgg tgcgaggatc gtgatgtatt tcgggttacg gcgcagcagc
     6061 ttttctgtct gatgaaccag atagtccaga ccgccagcaa cctgcatggc agaaatagcg
     6121 gcgataaccg ccatgataat ggaaatgaca tcgaatggga tgttaccggg tttaacgcca
     6181 atagcggcaa gaaccagcac ccccaatccg cctgcaaaac caatacctat tccccccaat
     6241 ctggcgccca agaagatcgc cagcaaaact atgatgagtt ctacaactag catattagcc
     6301 ttccttgttt tttaacaagt tgatattaaa ttgttatttt taagttactg ctcacaagaa
     6361 aaaaggcacg ccatctgacg tgccttcttt atttgtacta ccctgtacga ttactgttcg
     6421 ctttcatcag tatagcgttt tgctttgtaa gccgggtgca tcagattctg tacggagaaa
     6481 atatcgtcaa gttccgcttc agtcaacaga ccgcgttcca gaacgacttc acgtacactc
     6541 ttaccggttt cggcacagat tttacccacg atgtcaccgt tgtggtgacc gatgaacggg
     6601 ttcaggtaag taacgatacc gatagagttg taaacgtaac cttcgcacac ttctttgtta
     6661 gcagtgatgc cgttaatgca tttttccagc aggttgtagc aagcgttggt cagaatgtga
     6721 acggattcga acatggcctg gccaatgact ggctccataa cgttcaactg cagctgacct
     6781 gcttctgctg ccatggtaac agtggtgtcg ttaccgatga ctttgaagca tacctggtta
     6841 accacttccg gaacaaccgg gtttacttta gctggcatga tggaagagcc tgcctgcagt
     6901 tccggcaggt tgatctcgtt caggccggca cgtggaccag aagagagcaa gcgcaggtcg
     6961 ttacagattt tggacatctt cacagccagg cgtttcagcg cgccgtgaac cataacataa
     7021 gcgccgcagt cggaggtcgc ttcgatcagg tcttcagccg gtacgcatgg gaagccagtg
     7081 acttcagcca gttttttcac tgccagcgga gagtactctt tcggcgtgtt cagaccagta
     7141 ccgattgctg ttgcgccaag gttaacttcc agcagcagtt cagcggtacg ttggatattt
     7201 ttcacttctt ctttcagcaa gatgctgaag gcgcggaatt cctgaccgag ggtcatcggt
     7261 actgcgtcct gcagctgggt acgacccatt ttcaggatgt cctggaattc gacagcttta
     7321 cgttcaaagc cttcacgcag ttggttaatc gcatctacca gtttaatcag ggaagagtaa
     7381 actgcgatac ggaaaccggt cgggtaggcg tcgttagtgg actgacattt gttaacatgg
     7441 tcgttcgggt tcaggtactg atattcacct ttctggtgac ccatcagttc cagaccgata
     7501 ttggccagca cttcgttggt gttcatgttt acggaagtac ctgcgccgcc ctggtagacg
     7561 tctaccggga actgatccat gcattttccg ttgttcagga cttcatcaca tgcggcaatg
     7621 atggcattcg ctacactttt aggaatggtt tgcagctctt tgtttgccat agctgcggct
     7681 tttttaacca ttaccatacc gcgaacaaat tcaggaatat cactgatttt gttgttgctg
     7741 atatagaagt tttcaatcgc tctcagagtg tgaacaccat agtaggcatc agctggaact
     7801 tccctggtac ccaacagatc ttcttcgata cgaatgttgt ttgacatgtg aaccttcttt
     7861 ttcaagctgc caatgatttg ctttaaacac acagaatata tgtggtttcg aatgtttttc
     7921 gaccgacgat tatcccctgc atcgaccgaa tacccgagat catatgctgc ttgaggattt
     7981 ctaccgtaac ctggatcact ttaagtgtcg gtttttaccc cttaattatt aatttgtgaa
     8041 atggatcacc gctttgggat tacgaccaaa aatagttgcg caaacatctt gaaattttgc
     8101 taatgaccac aatataagct aaacgcgatt cgcaacccat tcaggtagcc gggtttaacc
     8161 ggctgctatt acaggagaaa cctttgcgct ggttaccttt tattgccatt ttcctttatg
     8221 tctatattga gatttcaatc tttattcagg ttgctcatgt attgggggta ttgctgaccc
     8281 tcgtgctggt tatattcacg tcagttatcg gtatgtcact ggtacgtaac cagggtttta
     8341 agaatttcgt gctgatgcag caaaaaatgg cggcgggtga aaacccagcg gcggagatga
     8401 ttaaaagtgt ttcgctgatc attgccggtt tgctgctttt attaccgggc tttttcaccg
     8461 acttcctcgg tcttctgctt ttattgccgc cggtgcaaaa gcatctgaca gtgaagttga
     8521 tgcctcatct gcgtttttct cgcatgccag gcggcggttt tagcgccggg accggtggcg
     8581 gtaatacttt tgatggtgag taccagcgaa aggatgatga ccgcgaccgc cttgatcata
     8641 aagacgatcg ccaggattaa tgtcgaaacg ccggattatg tggttatgcc atttcccggc
     8701 gtttttcgtt ttggcagaaa cagccataac cccgccagca tgatcagcgc gtagagactt
     8761 ttccagccga ccattgccag taacagaacg cataatagcc cgccaaccac tgccagtagt
     8821 cgataacgtc cttgcaataa tttacagcct gccagcatgc ataacagata aatcataata
     8881 aagatgccat tggcataaat aataagagcg tccagattga tctctaaagc atgaatcacc
     8941 aacgtgctca ccacgcagca gccgagcacc gcattgaggg cattattcgg aatatggcga
     9001 gaagagaggc gtgccaggta gtggtcagga ttatgttgcg cctgcgacca gaccaggcgg
     9061 gcgaagctct gtatataaat gttgagactg gcaaagcagg ccagatagcc aattacgcag
     9121 gcaatccata acgctcctac accgaacaac tgcactacaa ttttgggaag cgatgctgcc
     9181 gccgccattt gttcaccata ggcgtcgaag tgtaagacga ctaccgtaca gccccaatag
     9241 actaatcctg ccagcagcag gccaatcatc aaagcacgag gaaaatcacg ctctggattt
     9301 ttaaattccg aggcaagatg ggcaaatgct tccagaccga caaaacacca gaacatcact
     9361 gataacgcag cgaataaccc ggtaagttcg atatttcctg gcgcagggaa ggggatattc
     9421 gcaggtttga tatcgcccgc ccaccagata gcgacaatca gtgcgacgat aagcccggca
     9481 ataactgttt gtagattagc actggaactg gcacctcgag taccgatata ccacaccagc
     9541 gccagcgtgc cgagttctgc caacaacagt tgcccgctat gccagccaaa cattgcctgg
     9601 ccgaatccgg cagcaatttg tagcgcggca ggcaaaccca cgggaatgac cgataaaaac
     9661 aaccagccgg tgactcgctc aagccgcgaa ccaaacgcca taccgacgaa gtgcgcgacg
     9721 ccgcctgcgc tgggatagtg gcgacccaga atcgcaaaca caatcgcaat cgggaacact
     9781 aagataatca aaacgggcca cgcccacagg ctgttattgc ctgctactag cgcagctaac
     9841 gcaggaacgg caaacacgcc agtgcctaat aatgacgtcg atagcaggcc gatgccctgg
     9901 gccagcccca gttcttgttt gagtccactc atgggttgat gtccgattgc gcccaaattt
     9961 tgggcaactg cgtagatttt cgatggtagc acaatcagat tcgcttatga cggtgatgaa
    10021 gaaatagcga tgaaatgtga ggtgaatcag ggttttcacc cgattttgtg ctgatcagaa
    10081 ttttttttct ttttccccct tgaaggggcg aagcctcatc cccatttctc tggtcaccag
    10141 ccgggaaacc acgtaagctc cggcgtcacc cataacagat acggactttc tcaaaggaga
    10201 gttatcaatg aatattcgtc cattgcatga tcgcgtgatc gtcaagcgta aagaagttga
    10261 aactaaatct gctggcggca tcgttctgac cggctctgca gcggctaaat ccacccgcgg
    10321 cgaagtgctg gctgtcggca atggccgtat ccttgaaaat ggcgaagtga agccgctgga
    10381 tgtgaaagtt ggcgacatcg ttattttcaa cgatggctac ggtgtgaaat ctgagaagat
    10441 cgacaatgaa gaagtgttga tcatgtccga aagcgacatt ctggcaattg ttgaagcgta
    10501 atccgcgcac gacactgaac atacgaattt aaggaataaa gataatggca gctaaagacg
    10561 taaaattcgg taacgacgct cgtgtgaaaa tgctgcgcgg cgtaaacgta ctggcagatg
    10621 cagtgaaagt taccctcggt ccgaaaggcc gtaacgtagt tctggataaa tctttcggtg
    10681 caccgaccat caccaaagat ggtgtttccg ttgctcgtga aatcgaactg gaagacaagt
    10741 tcgaaaacat gggtgcgcag atggtgaaag aagttgcctc taaagcgaac gacgctgcag
    10801 gcgacggtac caccactgca accgtactgg ctcaggctat catcactgag ggtctgaaag
    10861 ctgttgctgc gggcatgaac ccgatggacc tgaaacgtgg tatcgacaaa gcagttaccg
    10921 ctgcagttga agaactgaaa gcgctgtccg taccgtgctc tgactctaaa gcgattgctc
    10981 aggttggtac catctctgct aactccgacg aaaccgtagg taaactgatc gctgaagcga
    11041 tggacaaagt cggtaaagaa ggcgttatca ccgttgaaga cggtaccggt ctgcaggacg
    11101 aactggacgt ggttgaaggt atgcagttcg accgtggcta cctgtctcct tacttcatca
    11161 acaagccgga aactggcgca gtagaactgg aaagcccgtt catcctgttg gctgacaaga
    11221 aaatctccaa catccgcgaa atgctgccgg ttctggaagc tgttgccaaa gcaggtaaac
    11281 cgctgctgat catcgctgaa gatgttgaag gcgaagcact ggcaactctg gttgttaaca
    11341 ccatgcgtgg catcgtgaaa gttgctgcgg ttaaagctcc gggcttcggc gatcgtcgta
    11401 aagctatgct gcaggatatc gcaaccctga ctggcggtac cgtaatctct gaagagatcg
    11461 gtatggagct ggaaaaagca accctggaag acctgggtca ggctaaacgt gttgtgatca
    11521 acaaagacac caccaccatc atcgatggcg tgggtgaaga agctgcaatc cagggccgtg
    11581 ttgctcagat ccgtcagcag attgaagaag caacttctga ctacgaccgt gaaaaactgc
    11641 aggagcgcgt agcgaaactg gcaggcggcg ttgcagttat caaagttggt gctgctaccg
    11701 aagttgaaat gaaagagaaa aaagcacgcg ttgaagacgc cctgcacgcg acccgtgctg
    11761 cggtagaaga aggcgtggtt gctggtggtg gtgttgcgct gatccgcgta gcgtctaaac
    11821 tggctgacct gcgtggtcag aacgaagacc agaacgtggg tatcaaagtt gcactgcgtg
    11881 caatggaagc tccgctgcgt cagatcgtcc tgaactgcgg cgaagaaccg tctgttgttg
    11941 ctaacaccgt taaaggcggc gacggcaact acggttacaa cgcagcaact gaagaatacg
    12001 gcaacatgat cgatatgggt atcctggatc caaccaaagt aacccgttct gctctgcagt
    12061 acgcggcttc tgtggctggc ctgatgatca ccaccgagtg catggttacc gacctgccga
    12121 aaaacgatgc agctgactta ggcgctgctg gcggtatggg cggcatgggt ggcatgggcg
    12181 gcatgatgta attgccctgc acctcgcaga aataaacaaa cccccgggca gaaatgtctg
    12241 ggggtttttc ttttggtcat ctttctagta taagattcag acacggacga cgcgagtggc
    12301 gtccagctca ttgattatgg ggaataacat gcacgtaaaa tacttagcag ggattgtcgg
    12361 tgccgcgcta ctgatggcgg gttgtagctc cagcaacgaa ttgagtgctg ccggtcagag
    12421 tgtacgcatt gtggacgagc aaccaggtgc agagtgccag ctgattggta ctgcgacagg
    12481 taagcaaagc aactggcttt ccgggcaaca cggagaagaa ggcggttcta tgcgcggcgc
    12541 agcaaacgat ctgcgcaacc aggcggctgc aatgggcggt aacgtgattt atggcatcag
    12601 cagcccgtcg cagggaatgt tgtccagttt tgtcccgacg gatagccaga ttatcggtca
    12661 ggtttataag tgcccgaact gatgtgatcc agaggctgga tacttccagc ctcgtagtaa
    12721 aacagatgag attatttcgc ctgtggtgca gttttgctgg tggattgaac acgtaagtga
    12781 tgcagcgcgc gtaagcattg atcctgggtt agcggttggg cgttatttgc cgctacggtg
    12841 cgaacataga cctgacatgg cacacccgcg agttttttca gtcgggggct gaaatcaagc
    12901 attcggcgag tgatcgcctc cacttcttta cccggcgttg cttcacgcgt tttcaccact
    12961 gcgccgctaa attgttcatt gccactttca tctttaaagc ggtaaagcac cgccagataa
    13021 tggttgaaca agttatgttt ccattccggt tgggaatagg tgtcatattc cagattgcca
    13081 ttttcctggc agtcaacgtc gtataacggc aggaaatcta acagtgaggt aatacgtaat
    13141 gccagttcac gcatttccgc attattaatt gcatgaatga ttgctcgcgc cagcatttca
    13201 atctgcaact cattgacgac taactcacat ttgctaccgt catgtagcgt taatacgaag
    13261 gtaagatttt cgccctgatc atcggtaagt tccagcgtgt taacccgacg attaatatcg
    13321 gcatttttaa gctcatcaac cagaatttct gggatatttt ctgccatttt cttaataact
    13381 ttatcgcgag cttgttcata ctgaaggcgt gcagcagcat ccagtttatg cttttgatgc
    13441 agacgacttt ccagcgcaat cagaaggtcg cgcaattcca tggcagacat aaagaacaac
    13501 gattctttat tgcgaggttc tttaattttc agcgccagtg caatgaaatt attactcttg
    13561 cgaataacac cggtattaac acccttgatg cttatggcca tgtaggttct ccctaaccat
    13621 ttctcaatta aataattaat tttagtttat aagccagata aatgggcttg gtagtaatag
    13681 ttgttaaaat aacataaata gccgtgctca ctctatataa acagaaaaga aaggttaatt
    13741 attggtgttg gttatataaa gagtaacaac agcaatgtat atgaataatt aaaggttatg
    13801 caacgggcaa agattaaact tccctgttta atcagttact gctggcgtag ctggagatcc
    13861 agcggcgttt tgctgggttc gccgccaatt tctcgcgcca gtttaggcac cagatatccc
    13921 gacaccaatg tcagcaactc acgcataatc tgccgtgctt cgtcatcact caccataaaa
    13981 tgcgccgcgc cctgtacttt atcgagcaca tgcaggtaat acggcatcac gcccgcatcg
    14041 aacaacgcat tactcaggtt tgccagcgtt tgcgcgttat cgttcacacc acgtaacaga
    14101 acgctctggt tcagcaaagt aacgcccacc cggcgcaact tagccatcgc ctgacggaat
    14161 gtttcatcta cctcattggc atggttgatg tgattcacca gcaagatttg cagcgtagaa
    14221 tgagaaaagc gttcaaccag cgcatctgtg atgcgtgccg ggatcacaat cggcagacgg
    14281 ctgtgaatcc gcagacgttt gatatgcggg atggcttcca gttgtgtgag caaccagtcc
    14341 agctcgtgat ctttcgccat cagcggatcg ccgccggaga aaatcatctc gtccagttcc
    14401 ggatgcgcag caacatactc aagtgcagtc tgccagttac gcttgttgcc ctgattttcg
    14461 gcatagggga agtggcgacg gaagcaatag cggcaattta ccgcgcagcc gcctttgacc
    14521 agcaaaagcg cccggttgtg gtatttatgc aacaaaccag gcactacgct atgctgttct
    14581 tccagcgggt cggtggagaa tccgggcgcg acgacaaact catcttgcga ggtaagtacc
    14641 tgacgcaaaa gaggatcgtc cggattgcct ttctccatgc gatcgataaa tgagcggggc
    14701 acacgcaggg caaacagctt tttggcgctg cgtccggcca acagtttttc gtccgcgtct
    14761 atattcaaaa gacgcagaag ttcatcagga tcggtcacaa catcggcaag ttgcgttaac
    14821 caatcttctc tggatggggt atttagggtt acaatatgcg ccattttgtg gcttagctac
    14881 caattaacaa atttcagagg gccttatggc aacgtactat agcaacgatt ttcgtgctgg
    14941 tcttaaaatc atgttagacg gcgaacctta cgcggttgaa gcgagtgaat tcgtaaaacc
    15001 gggtaaaggc caggcatttg ctcgcgttaa actgcgtcgt ctgctgaccg gtactcgcgt
    15061 agaaaaaacc ttcaaatcta ctgattctgc tgaaggcgct gatgttgtcg atatgaacct
    15121 gacttacctg tacaacgacg gtgagttctg gcacttcatg aacaacgaaa ctttcgagca
    15181 gctgtctgct gacgcaaaag caattggcga caacgctaaa tggttgctgg atcaggcaga
    15241 gtgtatcgta actctgtgga atggtcagcc gatctccgtt actccgccga acttcgttga
    15301 actggaaatc gttgataccg atccgggcct gaaaggtgac accgcaggta ctggcggcaa
    15361 accggctacc ctgtctactg gcgctgtggt taaagttccg ctgttcgtgc agatcggcga
    15421 agtcatcaaa gtggataccc gctctggtga atacgtctct cgcgtgaagt aatgcggttg
    15481 tggtgcggcc tgcaggctgc accatcactt tttcaggtca gagatgatga aacgccttat
    15541 cgttcttgtt ttgcttgcca gcgcgctgct cacgggctgt aacaccgctc gcggtttcgg
    15601 cgaagatatc aaacatctcg gcaactccat ctcccgcgct gccagctaat ttttcttctc
    15661 ctccgaaaaa tcatcagatt tccatcattt ttggcgatgt tgtctattat taatttgcta
    15721 taggcaaaca taaataacat tacctaaaag gaagacgtta tggtgaagaa gacaattgca
    15781 gcgatctttt ctgttctggt gctttcaaca gtattaactg cctgcaacac cacgcgtggc
    15841 gttggtgaag acatttctga tggcggtaac gcgatttctg gcgcagcaac gaaagcgcag
    15901 caataagcaa taacggtacg acagctgtgt cgtgccgttt gttttttctg cgatagttac
    15961 ataggtaata gttgaaattc ccctgccacc tggcaaaata tccgttcaac catcagcttt
    16021 gcaggacgac ctgcaaacgc gtcttttcac cggggacggc cccaattttc tggagcctga
    16081 tatgtcctgg attatcttag ttattgctgg tctgctggaa gtggtatggg ccgttggcct
    16141 gaaatatact cacggcttta gtcgtttgac gccaagtgtt attaccgtga cggcgatgat
    16201 tgtcagtatg gcgctacttg cctgggcgat gaaatcgtta ccagtaggga cggcttatgc
    16261 cgtgtggacg ggtatcggcg cagtcggcgc ggccatcacc ggcattgtgc tgctcggtga
    16321 gtccgctaac ccgatgcgcc tggcgagtct ggcgttaatc gtattgggga ttattggtct
    16381 gaaactcagc actcattaac taccgggctg ctgtacccaa ataaacttac tgacatcaaa
    16441 cccttcccgg gtcgcgactg ccagcatctc ctgtttcact tcgtcagaaa tggttggcgt
    16501 gcgggagagt atccacaggt agtcgcggtc cgggccgcaa accagcgcat ggcggtattc
    16561 ccgatcgagt gcaataacgt tataaccgcc atagaaagga ccaaagaatg acactttcag
    16621 cgcagcgcga gttggtgcgc cggtaaagta cgctttccct tcactctgct gccacatttc
    16681 tctgtcaggg ttatagcctt tattaatgac attcaggccg ccgtcatcac gcaggctgta
    16741 tgttgcggtg actttttcca gtccacgttc aaagcggtga tcaaaacggg caatctcata
    16801 ccaggtaccg agataacgtt tagcgtcgaa attattcact acggtcacgc cacgcggcgg
    16861 cgtaggggaa ctgcaggcaa cgaccagaaa tgcagctgtc gctgcggcaa cgagagggag
    16921 caggcgcata aatgtttcct tactggtttt tttctaagtg tagatgacag caaggaaaag
    16981 cggagaaaag gtccgaaaat tcggacccga tgaaatttta ctgtagagcg ttgagaatct
    17041 gccaggcggc ggcgactctc gctggattgg ggtagttttt gttagccagc atcacgatac
    17101 ccagctcttt ttctggaata aacgcgacat agctaccaaa tccgccggtc gcccccgttt
    17161 tatgtaccca tgatgcgcgt actgcaggag ttgggggcgt aatcggtttt acggggcgtg
    17221 ctgccagtgc aattttattg tcactgccgt taatgatgat gtcaggattt accggccagt
    17281 ccagcatttc ccagcccaga ccctgataca tatcgccggt ttgccagtag cgagattgtg
    17341 ccagttgtat cccttgttga agtgttttct cattgatatc aaggggtttt aaattgcttt
    17401 gcacccagcg ggccatatct tcaatggtcg acttcacacc ataagcttca gcatctaacg
    17461 cccctggcga aacatgtact gccttacctt cgcgatatcc ccaggcgtag ttcttttctt
    17521 ctgcggacgg tacattaatc cacgtatgat tgagtttgag tggctggaag acacgagttt
    17581 tcatcgcctg ctcaaaactc aaaccagacg gcttcacagc cagtgcgccg aacaaaccga
    17641 tactggagtt ggcatacaga cgttgtgttc ctggagccca tgcaggctgc cagttttgat
    17701 agaagcgcag caagtcgctt gaggatttca cctcatccgg cacctgcaat ggcaggccgc
    17761 cagcggtgta ggttgcgaga tgtaatagtg tgatcccatt ccactgttta gcggtaagtt
    17821 caggccagta ttttgttgtg ggatcgctta acttgatttc ccctcgagca atagcgtcgc
    17881 caccaagcac gcccgtaaat gttttgctga ccgaacctaa ctcaaacaac gtttgctgtg
    17941 tgacgggctg ctttttggcg atgtccgcat agccccaggt aaagtaataa ggtttaccct
    18001 gataaattac cgccaccgcc ataccgggga tcttttgttg ctctataagc ggggtaattg
    18061 tgcgatgcac aatatcgttg atttgttgag gggcggcaaa tgtggagcaa gaggcggtaa
    18121 ttaataaggt gcagagtgtc gttttgagca taaggtctgg tttccataca aaacggcccg
    18181 ccataggcgg gccggattta cattggcgat gcgttagatt gtaacgacac caatcagcgt
    18241 gacaactgtc aggatagcag ccagaccgta gaaaacccat ttacccgcag gtacgtggat
    18301 tttcagatcg tgcatcgcgt ggtgcatacg gtgtaaacca caccacagcg gcagaacgat
    18361 catcaggaac aggaatacgc gaccaatgaa gctctgcgcg aacgccagaa cgcgctcgta
    18421 gctcagtgca tcacccggaa acagccctag cggcagcaga ataccaacca gcaggatcat
    18481 caccggcgca atgatggcgc tccacatacc accggccccg aagaggcccc agaataccgg
    18541 ttcgtcagaa cgctttggat ttggattaat catctcaggc tccttaccag tacagggcaa
    18601 caaacaggat tacgatggtg gcaaccacag ttaccgccca gagacttttg ataattggct
    18661 ctggtcccat tttttcgtct tttacaatga tattggccgc ttttggtgcc agctcaaacc
    18721 aggttttggt gtgcagcagg gctgccgcca gagtgatcag gttaatgatc atgataaccg
    18781 ggttttgtaa aaagtcgacg aatcccgccc aggcttccgg gccatttttc agggcaaaca
    18841 gcccgaaaat cagttcaatg ctgaaccaca cagccggaac cgccgtgcct tcgcgcagca
    18901 tgtaaaagcg ataaaacggc aattttttcc accaggtgga cgtcattggc cgtacatacg
    18961 gtttacgttt agtcgtcatg ttgcactcct tagcgtggtt tcagggtcgc gataagaaag
    19021 tctttcgaac tttctacttt gccctgctga atggccgcag ccggatcgac gtgtttcggg
    19081 cagacttcgg agcagtagcc cacgaaagta cagctccata cgccgttctg gctgttcaac
    19141 tgcgccatac gctccttctt accgtggtcg cggctatctt cgttataacg atgcgccagc
    19201 gtaatggcag ccggaccgat gaactctggg ttcaggccaa actgcgggca cgcggcgtag
    19261 cacagaccac agttgatgca accggagaac tggtgatact tcgccatctg cgccggggtc
    19321 tggatgttag taccctgatc cgcggtgcgg gagttgccga tgatgtacgg tttgatcgct
    19381 tccagacttt cgatgaagtg agtcatatcg accaccagat cgcgttcaat cgggaagtta
    19441 gctaacgctt caaccttcat accgtcggtg taatcacgca ggaaggtttt acatgccagt
    19501 tttggcacgt tgttaaccat catgccgcag gagccacaaa tcgccatacg gcaggaccag
    19561 cggtagctca ggtccggtgc caggttgtct ttgatgtagc ccagcgcatc cagtaatgag
    19621 gtagttgcgt cataaggcac ttcatagaat gcgctatgcg gtgcggtatc gacttccggg
    19681 ttatagcgca ccacctcaat tttcaggttt ttcatctcag ccattcgcct tctccttctt
    19741 attggctgct tccgccttat cggctgcatc cgcttcgcca ccgtaaacac gtttagctgg
    19801 cggcagcgta gtaatcttca cgtcgctgta ctccaggcga gtcgtgccat cagcatcgcg
    19861 gaaggcgagg gtgtgtttga ggaagttgac gtcgtcacgc tcggtgcaac cttcgtccag
    19921 acgctggtgc gcgccgcggg actctttacg tgccattgcg gagtgcgcca tacattcagc
    19981 aacgttcaga ccgtggccca gctcaatggt gtagagcagg tcggtgttga acacgctgga
    20041 agtgtcggtg atgcgcacgc gcttgaagcg ttcctgcagc tctgccagct tgtcgatggt
    20101 tttctgcatc agttccggcg tacggtagat accgcagcct tcttccatgg ccatgcccat
    20161 ttcgtcgcgg atcttagccc agttttcgcc gccatcctgg ttaaccagat ctttcagacg
    20221 ttgttcaacg ccagctgcct gcgcttcaat tgccgcttcg ttgccattac cggcagttgc
    20281 tgcacgctct gtcgcttgtt caccggccag acgaccgaag accaccagtt ccgccagcga
    20341 gttggagccc agacggttcg caccgtgcag accaacagag gaacattcac ccacggcgaa
    20401 cagaccttta atgcgggttt cacagttctg atcggtttcg ataccgccca tggtgtagtg
    20461 tgcggtcgga cgtaccggaa tcggttcttt aaccggatcg acgccaacgt acgctttcgc
    20521 cagttcgcag atgaacggca gacgttcatg cagttttttc tcgccgaggt gacgcaggtc
    20581 gaggtaaacc acatcgccac gtggcgtgga gatggtgttg cctttacgcc attcgtgcca
    20641 gaaggcctga gaaactttgt cgcgtggacc cagttccata tatttgtttt tcggctcgcc
    20701 cagcggagtt tccgggccca tgccgtaatc ttgcagataa cggtagccat ttttgttgac
    20761 cagaatacca ccttcaccgc ggcagccttc ggtcatcagg ataccggaac ctggtaggcc
    20821 ggttgggtga tactgaacga attccatgtc acgcagcgga acgccgtggc ttagcgccat
    20881 acccataccg tcaccggtaa cgatgccgcc gttggtgttg taacgataaa cgcgacccgc
    20941 accgccggta gccataacga ccgcgttagc acggatctgc accagcgtgc cttccatcat
    21001 gttcattgct accaggccgc gaacatgacc atcatcaacc agaatatcca gcacgaaatg
    21061 ttcgtcaaaa cgctggatct gcgggaattg cagagaggtc tggaacagcg tgtgcagcat
    21121 atggaagccg gtcttatcgg cggcgaacca ggtgcgctcg attttcatgc cgccgaagcg
    21181 acgtacgttg acgctaccat ccgggcgacg gctccatggg cacccccaca gttccagttg
    21241 ggtcatttcg gttgggcagt ggtggacgaa ataatccacg acatcctgct cacacaacca
    21301 gtcgccaccc gctactgtat cgtgaaagtg atattcgaag ctgtcatgat cctgcgcgac
    21361 agcggcggag cccccttctg cagcaacggt atggctacgc atcgggtata cttttgagat
    21421 tagtgcgatt tttgcatttg gatttgcctg cgcggcagca attgcagcac gtaatcccgc
    21481 gccaccggcg cctacaatgg caagatcggc ttgaaaggtt tgcacgacat tcctccagat
    21541 tgtttttatc ccacagcccc gtacttcagg gtaagtgcct gaaagttacg gtctgcgaac
    21601 gctattccac tgctccttta taggtacaac agtatagtct gatggataag tctgaaattt
    21661 gacgagatcg atttttttag tgcgcaagag ggctaaatta tcactgaaga tgattaattt
    21721 aattactaaa ccatcagatc gtgctttttt tagtcactgc ccaccgttgt ttgattcctg
    21781 cgtaaaattt gtctcgccgt cgcgttgcga gtagacttcg tgcccttgtc aaaaactgga
    21841 gatttaacta tgagcgaaac ggcatcctgg cagccgagcg catccattcc taacttatta
    21901 aaacgcgcgg cgattatggc ggagatccgt cgtttctttg ccgatcgtgg agtgctggag
    21961 gtggagacgc cttgtatgag ccaggcgacg gtaaccgata ttcatttggt cccgtttgag
    22021 acacgtttcg ttggccccgg gcattcgcag gggatgaatc tctggttaat gaccagcccg
    22081 gaataccata tgaagcgcct gctggttgcc ggttgtgggc cggtattcca gctgtgccgc
    22141 agcttccgta atgaagagat ggggcgttat cacaaccctg agttcactat gcttgagtgg
    22201 tatcgaccgc actatgatat gtaccggttg atgaacgagg tggacgatct cttgcaacag
    22261 gtgctggact gtccggcagc agaaagcctt tcttatcaac aagctttctt acgttatctg
    22321 gaaattgacc cactctctgc cgacaaaacg caactgcggg aagtggcagc gaaactggat
    22381 ttgagcaacg ttgccgatac tgaagaagac cgcgacacgc ttctacaatt gctgtttacc
    22441 tttggcgtag agccaaatat tggcaaagaa aaaccgacct ttgtgtacca ctttccagcc
    22501 agccaggcat cactggcgca aatcagtacc gaagatcatc gggtcgctga acgctttgag
    22561 gtttattata aaggtattga gctggcgaat ggtttccatg aattgacgga tgcccgtgag
    22621 cagcaacaac gctttgaaca agataaccgt aagcgcgcgg cgcgcggttt gccgcagcac
    22681 cccattgacc agaatctgat tgaagccttg aaagtcggta tgcctgactg ttccggcgtg
    22741 gcattaggcg ttgatcgtct ggtgatgttg gcgctgggcg cggagacact ggctgaagtc
    22801 atcgccttta gcgttgaccg ggcataattc tgaaaaatta ctgaattaac cgcgaagaat
    22861 ggcgtgataa gcgccattct gtagcatatg ttttcatttt tccgctaaag gccacaattt
    22921 aagactggat atttgatatc atccaggtat caattctgta ttggttttta cgctgcccgc
    22981 tcaatgttgg gcagtgcagt tcagtgggcg cgttttatac acgcgctgaa atgaaggatg
    23041 gtttcatgcc tcacacgata aaaaagatga gtctgatagg actcatattg atgatcttta
    23101 cttctgtatt tggatttgcc aatagcccat cggcttatta cttaatgggt tatagtgcga
    23161 ttccctttta tatattttct gcattgttat tctttattcc attcgcctta atgatggctg
    23221 aaatgggagc tgcttatcgc aaagaagaag gcggtatcta ttcctggatg aataatagtg
    23281 tcggaccacg ttttgccttc attggtacgt ttatgtggtt ttcctcttat atcatctgga
    23341 tggtgagtac ctccgcgaaa gtttgggtac cgttctcaac attcctctat ggtagcgaca
    23401 tgacccagca ctggcgtatt gccggactgg agcctacgca ggtggttggt ctgctggcag
    23461 tggcatggat gattctggtc accgtcgttg cttctaaggg gattaataaa attgcccgca
    23521 ttactgcggt gggcggtatt gcagtaatgt gtctgaattt agtattgctg ttagtaagca
    23581 ttactatttt gttattaaat ggtgggcatt tcgcgcagga tattaatttc cttgcatcac
    23641 cgaaccc
//
LOCUS       DAZFFE010000074        23547 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFFE010000074 DAZFFE010000000
VERSION     DAZFFE010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.074"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(5..421)
                     /gene="higA"
                     /locus_tag="ACHJ69_003119"
     CDS             complement(5..421)
                     /gene="higA"
                     /locus_tag="ACHJ69_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO5016052.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(418..732)
                     /gene="higB"
                     /locus_tag="ACHJ69_003120"
     CDS             complement(418..732)
                     /gene="higB"
                     /locus_tag="ACHJ69_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFO5016053.1"
                     /translation="MHLITQKALKDAAEKYPQHKAELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(1017..2153)
                     /gene="rlmG"
                     /locus_tag="ACHJ69_003121"
     CDS             complement(1017..2153)
                     /gene="rlmG"
                     /locus_tag="ACHJ69_003121"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO5016054.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            2238..2741
                     /gene="ygjP"
                     /locus_tag="ACHJ69_003122"
     CDS             2238..2741
                     /gene="ygjP"
                     /locus_tag="ACHJ69_003122"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO5016055.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            2818..3510
                     /gene="ygjQ"
                     /locus_tag="ACHJ69_003123"
     CDS             2818..3510
                     /gene="ygjQ"
                     /locus_tag="ACHJ69_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO5016056.1"
                     /translation="MLRVFAHLLRRVCFSRRTLKIGCLLLLIAGATLFIADRVMVNAS
                     QQLTWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRANKVFSENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGLYTQLREKLARVSAVIDAKILHRQPKYLGPLVMI
                     GPFSEHGCPAKE"
     gene            3589..4575
                     /gene="ygjR"
                     /locus_tag="ACHJ69_003124"
     CDS             3589..4575
                     /gene="ygjR"
                     /locus_tag="ACHJ69_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO5016057.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTAYLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPTFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVNHPGLAVSRITAKLLTEIRRQTGVIFPADNVKL"
     gene            4858..5823
                     /gene="alx"
                     /locus_tag="ACHJ69_003125"
     CDS             4858..5823
                     /gene="alx"
                     /locus_tag="ACHJ69_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO5016058.1"
                     /translation="MNTVGTPLLWGGFAVVVTIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVG"
     gene            6147..7391
                     /gene="sstT"
                     /locus_tag="ACHJ69_003126"
     CDS             6147..7391
                     /gene="sstT"
                     /locus_tag="ACHJ69_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO5016059.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLILGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIHFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAATCQAEDDRLANSALRN"
     gene            complement(7395..7946)
                     /gene="ygjV"
                     /locus_tag="ACHJ69_003127"
     CDS             complement(7395..7946)
                     /gene="ygjV"
                     /locus_tag="ACHJ69_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO5016060.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQRRGIDPFKVEKTPSAVDERG"
     gene            complement(8029..9516)
                     /gene="uxaA"
                     /locus_tag="ACHJ69_003128"
     CDS             complement(8029..9516)
                     /gene="uxaA"
                     /locus_tag="ACHJ69_003128"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO5016061.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIAHLHQLYSVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            complement(9531..10943)
                     /gene="uxaC"
                     /locus_tag="ACHJ69_003129"
     CDS             complement(9531..10943)
                     /gene="uxaC"
                     /locus_tag="ACHJ69_003129"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO5016062.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            11426..12724
                     /gene="exuT"
                     /locus_tag="ACHJ69_003130"
     CDS             11426..12724
                     /gene="exuT"
                     /locus_tag="ACHJ69_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO5016063.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            12854..13630
                     /gene="exuR"
                     /locus_tag="ACHJ69_003131"
     CDS             12854..13630
                     /gene="exuR"
                     /locus_tag="ACHJ69_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO5016064.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            13870..13953
                     /locus_tag="ACHJ69_003132"
     CDS             13870..13953
                     /locus_tag="ACHJ69_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016065.1"
                     /translation="MLMYQTRRTYQDSNNIAVVHHLKPAWR"
     gene            13975..14637
                     /gene="yqjA"
                     /locus_tag="ACHJ69_003133"
     CDS             13975..14637
                     /gene="yqjA"
                     /locus_tag="ACHJ69_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO5016066.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            14641..15024
                     /gene="mzrA"
                     /locus_tag="ACHJ69_003134"
     CDS             14641..15024
                     /gene="mzrA"
                     /locus_tag="ACHJ69_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO5016067.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVSTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            15171..15539
                     /gene="yqjC"
                     /locus_tag="ACHJ69_003135"
     CDS             15171..15539
                     /gene="yqjC"
                     /locus_tag="ACHJ69_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO5016068.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLKARDY"
     gene            15577..15882
                     /gene="yqjD"
                     /locus_tag="ACHJ69_003136"
     CDS             15577..15882
                     /gene="yqjD"
                     /locus_tag="ACHJ69_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO5016069.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGISAAIGVVLGVLLSRR"
     gene            15885..16289
                     /gene="yqjE"
                     /locus_tag="ACHJ69_003137"
     CDS             15885..16289
                     /gene="yqjE"
                     /locus_tag="ACHJ69_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO5016070.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLVIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            16279..16578
                     /gene="yqjK"
                     /locus_tag="ACHJ69_003138"
     CDS             16279..16578
                     /gene="yqjK"
                     /locus_tag="ACHJ69_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO5016071.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            16765..17157
                     /gene="yqjF"
                     /locus_tag="ACHJ69_003139"
     CDS             16765..17157
                     /gene="yqjF"
                     /locus_tag="ACHJ69_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO5016072.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGIQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGMNSLMFMKN
                     LTISGGFLLLAITGPGAFSIDRLLNKKW"
     gene            17227..18213
                     /gene="yqjG"
                     /locus_tag="ACHJ69_003140"
     CDS             17227..18213
                     /gene="yqjG"
                     /locus_tag="ACHJ69_003140"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO5016073.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     KGGFAAEKERYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQPKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            18496..18864
                     /locus_tag="ACHJ69_003141"
     CDS             18496..18864
                     /locus_tag="ACHJ69_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO5016074.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(18898..19794)
                     /gene="yhaJ"
                     /locus_tag="ACHJ69_003142"
     CDS             complement(18898..19794)
                     /gene="yhaJ"
                     /locus_tag="ACHJ69_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO5016075.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            19899..20600
                     /gene="yhaK"
                     /locus_tag="ACHJ69_003143"
     CDS             19899..20600
                     /gene="yhaK"
                     /locus_tag="ACHJ69_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO5016076.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKSLTRMQLWLDACPQRENPLIQKLALNMDKQQLIASPEGGMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGQFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            20623..20787
                     /gene="yhaL"
                     /locus_tag="ACHJ69_003144"
     CDS             20623..20787
                     /gene="yhaL"
                     /locus_tag="ACHJ69_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO5016077.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(20847..22157)
                     /gene="cyuA"
                     /locus_tag="ACHJ69_003145"
     CDS             complement(20847..22157)
                     /gene="cyuA"
                     /locus_tag="ACHJ69_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO5016078.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AISDAKALLAAGKVSVKIQEPCDEILFSRAKVWNGVKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVTEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGNWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGANDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(22185..23516)
                     /gene="cyuP"
                     /locus_tag="ACHJ69_003146"
     CDS             complement(22185..23516)
                     /gene="cyuP"
                     /locus_tag="ACHJ69_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5016079.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKIPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
BASE COUNT         5578 a         6123 c         6008 g         5838 t
ORIGIN      
        1 cgcgttaatc aataaacaag gcgggagaaa tgccgaatcg cgttgccagt tttttagcgt
       61 gttccagcgt taatttcctc ttcccgctca aaacgcgtga caccatcgat ttactgccaa
      121 tttccggaag atcggaaagg gttaaaccat attgatccat aagggtacga atcacagcta
      181 taccgccagg catggcttgc gccatggcat taaattcagc aaattcgggc gctgattctt
      241 cccacgcggt tattttggca cacaccagat ccagcaaggg gttttcagga tcgttgagca
      301 gcagatgatc taccagttcc agcgcctggg tgtattgttc ctcgttctga atacccgcca
      361 gaaaaggtgc cacagcagtt aacttttctc ctgcttgcaa gatgtcggca atcgcaatca
      421 ttttttcccc ttagtacgat gaacggcggt aaagaaatcg tattctttat gcgtcataac
      481 ttcacgtatg tagcactttt gcgattcaaa aaagaccatt gctacaacac gtaattcatt
      541 gcctccgaca ttgaaaacat aatgcttatc cagatatttg aagttatcca gagaagggaa
      601 tactgctttt aatgactcag gttttttgaa atatccctta gcaatcgtgt tccccagagc
      661 caccaactcc gctttatgtt gcgggtattt ttccgcagca tctttcaatg ctttttgagt
      721 tatcaggtgc attcttcatc acgtccgttg acaaattggc aatatgataa catccgttga
      781 cagattggca acgaatgaat ttatctgtgt gacggaagaa taaacgtaga aaaacgctaa
      841 acaagagaat gtagaggatg gtgacaggga ttttttgttt tatggaagcg cgattccaga
      901 actgggcaga cattgccgga tgcgacgctt gccgcgtctt atccgacctg ggggtaatgt
      961 gctgaacgta tgccggataa ggcgtttacg ccgcatccgg caggtaaccc ccaatcttac
     1021 cgacgacgcc ccagcttcac tgctttcagc accacaaatt tattattcgt ggcgatggtg
     1081 gtgcagttgc cgaaaatttt cttcagttta tggaagtaat ccaggtgacg gttggcaacg
     1141 atatacagct cgccgttgat tttcaggcag cggcgggcat ggtggaacat ctcccaggcg
     1201 acgttatcgg tcagcgcatg ttgctgatga aacggcgggt tgcagagcac agcattaaaa
     1261 cggaaaggct ccacgccgga gagcgcgttg ttgatcataa actcgcagcg atccaacgcc
     1321 tcgggcatgt tggtttcaac gttcaaacgg ctggaagcaa ccgccatcgg tgattcatcg
     1381 acaaacacca ctttcgcctg cgggttttta tcgagcaatg tcaggccaat aacgccatta
     1441 ccgcaaccga gatcgacaat ctcaccttcg agattctctg gcagatgttg cataaagaag
     1501 cgcgcgccaa tatccagccc agtgcgggag aaaacattcg catggttgtg gatagtccag
     1561 tcagtgcctt ccagcttcca gctaacggtc tgcggcgcat cggccaacgg cggttcgttg
     1621 aaggtgcagt taatcaggcg cgctttcttc catgccagcg tggtggtggt cggaccgagc
     1681 actttttcga acaattccag cgtggaagtg tgaatgtcac gggctttggc accggcaata
     1741 atgcgcgtat ctggcgtgac cactttgcgc agcgcgcgca gttgttgttc cagcaatgcc
     1801 agtgttttcg gcactttgat cagcaccaca cccggctgtt gcgggtagtc ggcggtgctg
     1861 tcgaggaact tcacgctcga ctcatcaatg ccgttgagac gtaaattctc acgcgtcgcc
     1921 aactcgctga tgtatgagtc gccaatgctg tatggcttat gttccgccag cgcacaactt
     1981 aacgcaccaa aggcatcatt caggatcaac accgggccgc ggatttctgt gtcgtccaac
     2041 tgttgcagca aatattcatc cgccgcttcc cacgcctgta gtgggtttac gtcatccgtc
     2101 gccggaaaac gttgtagtgt cagtgaacgg aaaccgttgt ctaagtggct catcggccct
     2161 cctgaatgat aaaatttcga cgttgcccct gaaaagggtg cgtgagtata ccctttttct
     2221 ctttttcgtg ggcggttatg agcaatctta cttatcttca gggttatccc gagcagctac
     2281 tttcccaggt gcgaacgctg ataaacgaac agcgtctggg cgatgtgctg gcaaaacgct
     2341 atccgggaac acacgactac gccaccgata aagccctctg gcaatatact caggatctta
     2401 aaaatcagtt tctgcgcaat gccccgccga tcactaaagt gatgtatgac aacaagatcc
     2461 atgtgctgaa aaatgcgctc gggttacata ccgctgtttc gcgtgtgcag ggcggcaagc
     2521 tgaaagcaaa ggcggagatc cgcgtcgcta ccgtgtttcg caacgcgccg gaaccgtttt
     2581 tgcgcatgat cgtggtgcac gagttggcgc acctgaagga gaaagagcat aacaaagcgt
     2641 tctatcaatt gtgctgccat atggagccgc agtaccacca gcttgagttc gacacccggc
     2701 tgtggctaac gcagttgtcg cttgggcagg acaaaatctg aaaatgatgt caggcgttat
     2761 tttttcctcc tttagcgcct aaaatcgacc tccccccttt cgttaaggat aacgaccatg
     2821 ctgcgcgtat ttgcccacct tcttcgccgc gtttgtttct ctcgccggac tcttaagatt
     2881 ggctgtttat tgttgcttat agcaggagca acccttttta ttgccgatcg cgttatggtc
     2941 aacgccagcc aacaactgac ctggagcgat gtcaacgccg ttccggcgcg gaacgtgggt
     3001 ttattgctgg gggcgaggcc gggtaatcgc tactttaccc gacgtattga taccgcagca
     3061 gcgttgtacc acgccggaaa agtgaaatgg ctattggtga gcggcgataa tggacgtaaa
     3121 aattacgatg aagcgtcagg aatgcagcaa gccttgatcg ctaaaggcgt accggcaaaa
     3181 gtgatcttct gcgactatgc cggattctca acgcttgatt cggtggtgcg tgccaacaag
     3241 gtgtttagtg aaaatcatat caccattatc tcgcaagaat ttcataacca acgcgccatc
     3301 tggctggcaa aacagtatgg tatcgatgct atcggtttta acgcgccaga cctgaatatg
     3361 aagcacggtt tatatactca actgcgggaa aaacttgcca gagtcagcgc ggtgattgat
     3421 gcgaaaatcc tccatcgcca gccgaaatat ctcgggccgt tggttatgat tgggccgttt
     3481 agtgagcatg gctgtccggc aaaagaataa tgcgtatctg cgcacgtcga agataaaaaa
     3541 ggcgtgctac agtgacgaga gaatcccttt atggagtatc cacgcgttat gatacgtttc
     3601 gctgtgattg gtacgaactg gatcactcgc cagttcgtcg aggccgccca tgagagcggt
     3661 aaatacaagt taaccgccgt ttattcccgc agtcttgaac aggcccagca cttcgccaat
     3721 gatttttctg tcgagcatct gtttacgtcg ctggaagcga tggcagaaag cgatgccatt
     3781 gacgcggtgt atattgccag cccgaattcc ctgcactttt cccagacaca acttttcctt
     3841 agtcacaaaa ttcatgtgat ttgcgagaaa ccgctggcgt cgaatctggc ggaagtggat
     3901 gccgccattg cctgtgcgcg ggaaaatcag gtggtgctgt ttgaggcatt taaaacggcc
     3961 tacctgccga actttcatct gttacgccag gcgctgccta aagtcggcaa actgcgtaaa
     4021 gtctttttca actattgcca gtattcctcg cggtatcagc gttacctgga cggagagaat
     4081 cccaacacct ttaatccgac attctctaac ggttcaatta tggatatcgg cttttactgc
     4141 ctggcgtcgg cggtggcatt attcggtgaa ccgaaaagcg tgcaggcaac cgccagtttg
     4201 ctggcaagcg gtgtggatgc ccacggcgtg gtggtgatgg attacggtga tttcagcgtt
     4261 acgttgcagc actcaaaagt gagcgattct gtcctggcaa gcgagattca gggcgaagcg
     4321 ggatcgctgg tgattgaaaa actgtctgaa tgccagaaag tctgctttgt accgcgtggc
     4381 agccagatgc aggatctcac ccagccacag catattaata ccatgctcta cgaagcagag
     4441 ctgttcgcta ccctggtgga tgagcatctg gtgaatcatc cagggctggc ggtcagtcgt
     4501 atcaccgcca aactgctgac tgagatccgc cgccagactg gggtgatttt tccggcagat
     4561 aacgtaaaac tataattgcc aaagtaacac agtgtaaaag gtatgtaaca gaccattgac
     4621 tgactgaatg gtctgtcata ctttgttacc tgcaaagggg agtaacttca ttgccggtcg
     4681 atcgtcatta cgatgtgtga aaaaccacat ccggtcaccg ggcaacccga aaggaatacg
     4741 cagacgtatt ccttttttgt tgtaagtgag accttgccgg aaggcgaggt ctatgcataa
     4801 aaagcagcgg ctgacgtctt ccgacgttgg ccgttttttt atgtgtaagg aacttctatg
     4861 aatactgtcg gcacgccgtt gctatggggc ggattcgctg ttgttgtcac cattatgctg
     4921 gctatcgacc tgttgttgca ggggcgtcgt ggggcacatg ccatgaccat gaaacaggct
     4981 gcggcctggt cgctggtctg ggtgacgctg tcgttactgt ttaacgccgc tttctggtgg
     5041 tatctggtgc aaaccgaagg tcgcgccgtt gccgatccac aggcactggc ctttctcacg
     5101 ggttatctga ttgagaaatc gctggcggtc gataacgtct ttgtctggct gatgttgttc
     5161 agttatttct ctgttccggc ggcattacaa cgccgcgtgc tggtgtatgg tgtactcgga
     5221 gcgattgttc tgcgtaccat catgatcttc actggtagct ggctgatttc acagttcgac
     5281 tggatcttgt atatcttcgg tgccttcctg ctgtttaccg gcgtgaagat ggcgctggct
     5341 catgaagatg aatcaggcat tggcgacaag ccgctggtgc gctggctacg cggtcatttg
     5401 cgcatgaccg acaccatcga caacgagcat ttctttgtgc gtaagaatgg tttgttgtat
     5461 gccacgccgc tgatgctggt gctgattctg gtggagttga gcgacgtgat tttcgccgtg
     5521 gatagtattc cggctatctt cgccgtgacc accgatccgt ttattgtgct gacctcaaac
     5581 ctgtttgcga tcctcggcct gcgtgcgatg tatttcctgc tggcaggtgt ggcagagcgt
     5641 ttctcgatgc tcaaatatgg tctggcagtg attctggtgt ttatcggtat caagatgctg
     5701 attgtcgact tctaccatat tccgatcgcc gtctcgctgg gcgtggtgtt tggcattctg
     5761 gtgatgacgt ttattatcaa cgcctgggtg aattatcggc atgataagca gcgggttgga
     5821 tgatttttaa tctgcctaag ccgtgtaccc tgtcatcaac atgagcaccg ttttctccct
     5881 ctccccaaaa ggggcgaggg gactgaccga gtactttttt gacgttgtca tcagtctgga
     5941 agccgcacgt tgggctttat ttttatgtca aataaatgta accattaagt tgcaaaatat
     6001 gacctctctt taaaatccag catttttcgc ttcccgaagc tgtaactttc cttatactcg
     6061 accttgcaaa cactttgtta catcctgaaa gatgcgtcga cagaacgcac cagggatgtg
     6121 cgacaacaca atgaaaggat cgaaaaatga ctacgcaacg ttcaccgggg ctattccggc
     6181 gtctggctca tggcagcctg gtaaaacaaa tcctggtcgg ccttattctg gggattcttc
     6241 tggcatggat ctcaaaaccc gcggcggaag ctgttggtct gttaggtact ttgttcgtcg
     6301 gcgcactgaa agccgttgcc cccatcctgg tgttgatgct ggtaatggca tctattgcta
     6361 accaccagca cgggcagaaa accaatatcc gccctatttt gttcctctat ctgctgggca
     6421 ccttctctgc tgctctggcc gcagtaatct tcagttttgc cttcccttct accctgcact
     6481 tgtccagtag cgcgggtgat atttcgccgc cgtcaggcat tgtagaagtg atgcgcgggc
     6541 tggtaatgag catggtttcc aaccccattg acgcgctgct gaaaggtaac tatatcggga
     6601 tcctggtgtg ggcaattggc ctcggtttcg cactgcgtca cggtaacgaa accaccaaaa
     6661 acctggtcaa cgatatgtcg aatgccgtta cctttatggt gaaactggtg attcacttcg
     6721 caccgatcgg tatttttggt ctggtttctt ctaccctggc aaccaccggt ttctccacgc
     6781 tgtggggcta cgcgcaactg ctggtggtac tggttggctg tatgctgctg gtggcgctgg
     6841 tggttaaccc actgctggta tggtggaaaa ttcgtcgtaa cccgttcccg ctggtgctgc
     6901 tgtgcctgcg cgaaagcggc gtgtatgcct tcttcacccg cagctctgcg gcgaacattc
     6961 cggtgaatat ggcgctgtgt gaaaagctga atctggatcg cgatacctat tccgtttcta
     7021 ttccgctggg agccaccatc aatatggcag gcgcagcaat caccattacc gtgttgacgc
     7081 tagctgcggt taatacgctg ggtattccgg ttgatctgcc cacagcgctg ctgttgagcg
     7141 tagtggcttc tctgtgtgcc tgtggcgcat ccggcgtggc gggggggtct ctgttgctga
     7201 tcccactggc ctgtaatatg ttcggtattt cgaacgatat cgccatgcag gtggttgctg
     7261 tcggctttat tatcggcgta ttgcaggact cctgtgaaac cgcgctgaac tcgtcaactg
     7321 acgtgctgtt cactgcggca acttgccagg cggaagacga tcgtctggca aatagcgccc
     7381 tgcgtaacta atacttagcc cctttcgtct acggcggaag gggttttctc cactttaaac
     7441 ggatcaattc cccttctctg catacgccag aaacgaatga tattcaggcc attcatcagc
     7501 agaaaactgc cttcaatcat cgtgccgcct atcgaccccg cccagaagtt gtgaatcacc
     7561 cagcaacacg ttgaaaacca cattacgcag cgcatggtca gccctttaca gcggaacagc
     7621 gcccaggtac tgacaatcgt gccgattacc ggcaatagtt cgacaggatg atggaacttc
     7681 gcgaggccaa ttccgccagt cagcacaata aaaatcgcca ttacccataa gctgcgcgtg
     7741 cgtaaggtaa tcaatgtacg aatggcatta aggatggcac tggcaccggc aggataggtg
     7801 cccagaagaa aaaaatgtac gccaataacg gcgctataga ccgaaagttg ctttttgaag
     7861 cgacgttcgt cacgattgaa aaatgttgtg ataccaatca gaaaggcgat gacacccacg
     7921 ccctgggcca gccaatacgc ggtcatgata aatccttagc aggtatggaa aagcaaacgg
     7981 cgcttcacat tatgaaacgc cgttttttat taacaactca tttcgacttt atagcgttac
     8041 gccgcttttg aagatcgcca gttcgcggaa gtcgttacgc tcgttacagg tttgcttacc
     8101 gttggcaaac tcaacgatag tgtcgataaa ttcttccagc aactgcggca tcgctttacc
     8161 gtggatcagc tgacccgcgt caaagtcgat ccagtgtttt ttcttcgccg ccagttcgct
     8221 gttggtggcg attttcaccg tcggtacaaa gccgccgtac ggcgtgccgc gaccagtact
     8281 gaacagcacc atatggcagc ccgcacccgc cagggcgctg gtcgctacgg catcgttacc
     8341 cggcgcactt aacaagttca gccccggcgt tttcagacgc tcgccgtaac gcagcacgtc
     8401 aaccacgaca ctggaacccg ctttctgggt acagccaagc gatttatctt ccagcgtggt
     8461 gataccgcct gctttgttcc ccggtgatgg gttttcgtag atcggctggt catgggcaat
     8521 aaagtactgc ttgaagtcat tgaccatggt gaccagtttt tcaaacgttg cttcgtcgcg
     8581 acaatggtcc atcagcaact gctctgcgcc aaacatctcc ggcacttcgg tcagtacggt
     8641 agtaccgccg ttagcaatca cgtagtcaga gaaacgcccc agcatcgggt tagcagtaat
     8701 accagaaaga ccgtcagaac caccgcactc cagaccaaac ttcagttcgc tgagtttgcc
     8761 cggctcgcgt ttatcgttgc gcatcacgct atacagctga tgcaaatgcg cgatcccggc
     8821 ctcgatttca tcatcctgct gttggcagat catgaaatga acgcgttcag gatcgatatc
     8881 gcccaacgtt tcacggaatg cggcaacctg gttattttcg caacccagac caatcaccag
     8941 cactgcgccc gcgttcgggt ggcgcaccat gttttgcagc atggtgcgag tattgatgtg
     9001 atcgtcgccc agttgtgagc agccgtaggt gtggctgaag aggaacacgc cgtcggtacc
     9061 ttcagcgttg ttggtctctt tcaggaaacg attttggatc tgccgcgcga tgccgttgac
     9121 acagcccacg gttggcagga tccacagctc attacgcacc ccgacatcgc cgttagcgcg
     9181 acgatagatc tgcacttcac gatctgccgc ttgcgcaggc agatcctgaa aatcaggttg
     9241 atagcgatac tgatccagat cgctcagatt cgtgcgcgta ttgtgggcgt gaacgtgttc
     9301 ccccgccgca atatccgcca atgcataacc aatcggcagg ccatatttaa tgacgttggc
     9361 cccttttgcg atatccgtta acgcaaattt atgtccacga gcaacatcct ggcgcagcgt
     9421 aacagtctgg ttatcgacac tgacttctgt gccttcagcc aaatccgcta aagcaaccgc
     9481 gacgttatcc agcgcatgga tcttgatgta ttgcatatca accccagacc ttagttcagt
     9541 tcaatggcga agtagtcacg cgcattgtta aagcaaatat ttttcaccat ctcgcccagc
     9601 aggttgatgt ccgccggtgc ttcgcccgct tccacccagc gaccgatcat ctggcacaga
     9661 atgcggcgga agtattcgtg acgggtgtat gacaggaagc tacggctgtc agtcagcatg
     9721 ccgacaaagc ggctcagcag accgagctgc gccagttggg tcatctgacg ttccataccg
     9781 tctttctggt cgttaaacca ccagccggaa ccgaactgca ttttgcccgg cataccttcg
     9841 ccctggaagt taccgatcat ggtgcccagc acttcgttat cgcgcgggtt caggcagtag
     9901 agaatggttt tcggcagcag gttttcttca ttctgcttgc tcagcagctt agacagctct
     9961 tccgccatcg gacggtcgtt gatggagtca aagccgacat ccggccccag cagtttaaac
    10021 tgacgcaggt tgttattacg cagtgcgcca atgtggtact gctgtaccca gccgcgacgt
    10081 gcatattcgg caccaaggaa caccagcacc gcagttttga actgtgccac ttcgtgctcg
    10141 ctcagggttt cgcctgccag acggcgcgcg aggatactgt ccagttccgc ttcgttcgct
    10201 tcagcaaaca tcaccacatc cagcgcgtgg tcagacactt tacagccgtg agcggcgaag
    10261 tgatccaaac gtttagtcag ggcagtttgc aggtcagcaa agcggcgaat gtcggtatcg
    10321 gaaacttcgc ccagcttcgc catgtagtcg ttaaaggtcg cctgttcaat gttaaaggct
    10381 ttgtccggac gccagctcgg cagcacttta atggtgaaag agccgtcttt ggcgatctct
    10441 gcgtgatgct ccagagaatc gatcgggtca tcggtggtgc cgaccatttt cacgttcatc
    10501 tgctgcatga tgccgcgcgc ggagaagtta tcctgcgcca gcagttcgtt acattcgttc
    10561 cagatttcat cggcagttga cggagaaagc aatttgccag tgataccaaa cggacgacgc
    10621 agttcgaggt gcgtccagtg gtataacggg ttgccaatag tgtgcggaac agtcgccgcc
    10681 caggcgtcaa atttttcacg gtcagacgca tcaccggtgc acagacgctc ggccacaccg
    10741 ttggtacgca tagcgcgcca tttgtagtga tcgcccttca accagatgtc atacaggttt
    10801 ttaaaacgat agtcttccgc aatctgctgc ggcggcaaat ggcaatggta atcgaaaatc
    10861 ggctggtctt ttgcgtagtc gtgatacaga cggcgggcaa attcagtatc taacaggaaa
    10921 tcttcagtca taaacggagt cattttcgtc ttcctctcaa cgagtgagct agcttgctta
    10981 tggtgcgatg ctgacaaagt tatcacacca atttccagag tccgaagata ttttcgtgag
    11041 ttagatcaat aaacgtagtt aaaaaaatta ctctcaaagt ggtaaatctc gctgcaggcc
    11101 gcgccagcac tggcctcgcc gtcgtcaggt aatgtcccta caaatattcg cacatttgtg
    11161 atggctctca ccttttaaag ttgtatgaca agttatcttt ctgccgtcgc aaatcataag
    11221 tcgacggaat gcaaattgcc gattcattca tttgttagat gaatcgggtt aaccggtacg
    11281 gaagccgaat tagcacgaaa ctttcatggc aacgttcggg gcgtgccggt tttttttcgg
    11341 ttacccggtc gtaactaaca tcttcagcct ctggcgggat gatggccgcg cttcctgcgg
    11401 atataacaaa acgatgaggt tttacatgcg taaaattaaa gggttacgtt ggtatatgat
    11461 cgcactggtg acgctcggca ccgtgcttgg ttacctgacg cgtaacactg tggcggcagc
    11521 tgcgccaact ctgatggaag agttaaacat ctccacccaa cagtattcct atatcatcgc
    11581 agcgtattct gctgcttata cggtcatgca accggtagcg ggttatgtac tggatgtgct
    11641 gggtacgaaa atcggttatg caatgtttgc tgtactgtgg gccgtgttct gtggtgcaac
    11701 tgcgctggca ggtagttggg gtggcctggc ggttgctcgt ggtgcggtcg gtgccgcgga
    11761 agccgcgatg attccggcgg gtctgaaagc cagctccgaa tggttcccgg cgaaagagcg
    11821 ttccatcgcg gtaggttact ttaacgtagg ttcttcgatt ggtgcgatga ttgcgccgcc
    11881 gctggtggta tgggcaatcg tgatgcacag ctggcagatg gcatttatca tctccggtgc
    11941 gttgagcttt atctgggcga tggcatggct gattttctac aagcatccgc gcgaccagaa
    12001 acatctgacc gatgaagaac gcgactacat tattaatggt caggaagccc agcaccaggt
    12061 tgacacagcg aagaaaatgt ccgttggtca gatcctgcgt aaccgtcagt tctggggtat
    12121 cgcgctgccg cgattcctgg cagaaccggc atggggtact tttaacgcgt ggatcccgct
    12181 gttcatgttt aaagtttacg gctttaacct gaaagagatc gcgatgtttg cctggatgcc
    12241 gatgctgttt gctgacctcg gttgtatcct cggtggttac ctgccgccgc tgttccagcg
    12301 ttggtttggt gtgaacctga tcgtttcccg taagatggtc gtaacgctgg gcgctgtgct
    12361 gatgattggc ccgggtatga tcggtctgtt caccaacccg tatgtcgcga ttatgctgct
    12421 gtgtatcggt ggttttgctc accaggcact gtctggcgca ctgattacgc tctcttctga
    12481 cgtgttcggt cgtaacgaag tggcgacggc gaacggttta accgggatgt ccgcatggct
    12541 ggcaagtacg ctgtttgcac tggtagtcgg tgcactggct gacaccatcg gcttcagccc
    12601 gctgttcgca gttctggcag tgttcgacct gttaggtgcg ctggttatct ggaccgtgtt
    12661 gcagaacaaa ccggcgattg aggtggcgca ggaatcacat aacgatcccg caccgcaaca
    12721 ttaaggcttc gtgtaagttt tagtttccct tcaaagccgc cttctcaggc ggctttttca
    12781 tcactgcgag tagagctaaa ctcgcaaaag tggtataaca aatatagtct gccgtatcat
    12841 gcctggagcg catatggaaa tcactgaacc acgccgtttg tatcaacaac ttgccgctga
    12901 cctgaaagag cgcatcgaac agggcgtcta tctggtgggt gataaactgc ctgccgaacg
    12961 ctttattgcc gatgaaaaaa acgtcagccg caccgtggtt cgtgaagcca tcatcatgct
    13021 ggaagttgaa ggctatgtcg aagtgcgtaa aggttcaggt attcatgtgg tttccaacca
    13081 gccgcgccat cagcaggcgg ctgacaataa tatggaattc gccaattacg gtccgtttga
    13141 gttgctccag gctcgccagc tcatcgaaag taatattgcc gagttcgcgg caactcaggt
    13201 cacaaaacag gacatcatga aattgatggc catccaggag caggcacgcg gcgaacaatg
    13261 cttccgtgat tccgagtggg atttgcagtt ccatattcag gtagccctgg cgacgcagaa
    13321 ctccgccctg gcggctatcg ttgaaaaaat gtggacccag cgtagtcata acccgtactg
    13381 gaaaaaactg cacgaacaca ttgatgcccg taccgtcgat aactggtgtg atgaccacga
    13441 tcaaatcctc aaggcgctga ttcgcaaaga tcctcatgct gctaagctgg caatgtggca
    13501 gcacctggaa aacaccaaga tcatgttatt taacgaaacc agcgacgatt tcgagttcaa
    13561 tgccgaccgc tatctgttcg ccgaaaaccc ggtggtgcat ctcgatacag ccaccagcgg
    13621 cagtaaatga aattcctgtc cgacaggcgc tacagtgcgc ctgtttgtct cgtcggtaag
    13681 cgaaaagtat aaagtgtcag cctgtgtaaa tcctctcgcc tccctcccct gcattcagca
    13741 aaatcagact ccacggacat gtaattttga taacgaacaa cgttgacctt tgttacaatt
    13801 agattcaatt tgaatttatg cttttgaatg ctttcttatc tcacgattta acagggaata
    13861 gttcaggctg tgttgatgta tcaaacccgc agaacatacc aagacagcaa taacattgcg
    13921 gtagtgcatc atttaaaacc agcgtggcgt taaccgattc accaggaata atgaatggaa
    13981 cttttgaccc aattgctgca agccctgtgg gcgcaggatt ttgaaaccct ggccaatccg
    14041 tcgatgattg gcatgttgta ttttgtcttg tttgtcattt tgttccttga aaacggcttg
    14101 cttccggcgg cctttttacc gggcgacagt ttactggtac tggtcggcgt attgattgcg
    14161 aaaggcgcga tgggctatcc gcaaacgatt ctgctgctga ccgttgccgc cagcctcggt
    14221 tgctgggtca gctatattca ggggcgatgg ctgggcaata cccgcaccgt acaaaactgg
    14281 ctatctcatt tacccgcgca ttatcatcaa cgcgcacacc atctttttca taaacacggt
    14341 ttatcggcgc tgttaattgg tcgctttatt gcgtttgtca gaacactgct gccgacgatt
    14401 gccgggttat cagggctgaa taacgcgcgc tttcagttct tcaactggat gagtggtctg
    14461 ctgtgggtgt tgatcctgac aactctgggt tacatgctcg gcaaaacgcc ggtattttta
    14521 aagtacgagg accagctgat gtcatgcctg atgctgctcc cggtggtgct gctggtgttt
    14581 ggcctggcag gttctctggt cgtgttatgg aaaaagaaat atggaaatcg ggggtaaggg
    14641 atgcaaatac ctcgcatgtc acttcgccag ctagcctggt ccggcgctgt tttacttctg
    14701 gtcagcacac tattgctggc ctggtccgcg gttcgccagc aagagtctac gctggcgatt
    14761 cgtgccgttc atcaagggac aacgatgcca gacggttttt caatctggca tcaccttgac
    14821 gctcacggca ttcctttcaa aagtatcacc cccaaaaacg acactctgtt aattaccttt
    14881 gactccagcg accagagcgc cgccgcaaaa gcggtcctcg acagaacatt gccccatggc
    14941 tacatcattg cgcagcagga caataacagt caggctatgc agtggctgac ccggttgcgg
    15001 gataactctc atcgcttcgg ataacttcct gttatccgaa acagatcact cactttggtg
    15061 atttcaccgt aactgtctat gattaatgag gcggtggtta acactaccgc ttcgttgtct
    15121 ggacaatcgt tcctttgtaa taggtccgaa tcacaatgga aggttcaaga atgaaatacc
    15181 gcatcgcttt agctgtttct ctctttgctc ttagtgccgg tagttatgcc actaccctgt
    15241 gtcaggaaaa ggagcaaaat atccttaagg agatcagcta tgccgaaaaa caccaaaacc
    15301 agaatcgtat tgacggtctg aataaagccc tgagtgaagt ccgggccaac tgttcagata
    15361 gccagctgcg tgccgatcat cagaagaaaa tcgcaaagca gaaagatgag gtggcggaac
    15421 gccagcaaga tttagccgag gcgaagcaaa aaggcgatgc agataagatt gccaaacgcg
    15481 aacggaaact ggcagaagcg caggaagagc tgaaaaagct gaaagcgcgc gactactaac
    15541 tcacaatagt cactacttac tcacctggag aaaactatgt cgaaagaaca cactacggaa
    15601 catctgcgtg ctgagttgaa atccctttcc gatacgctgg aagaggtgct tagctcatct
    15661 ggcgagaagt caaaagaaga gttgagtaag attcgtagca aagcggagca ggcactgaaa
    15721 cagagccgtt atcgcctggg tgaaaccggt gatgccattg ccaaacaaac ccgtgtcgcg
    15781 gcggcgcgtg ccgatgagta tgtgcgtgaa aatccgtgga cgggcgtggg cattagcgca
    15841 gcaatcggtg tagtgctcgg cgttctgctg tcgcgtcgtt aattatggcg gacactcatc
    15901 acgcgcaagg gcccggtaaa agcgttctgg gcatcgggca gcgaattgtt tccatcatgg
    15961 ttgaaatggt ggagacacgt ctgcggctgg cggtggtgga gctggaagag gaaaaagcga
    16021 atctctttca acttttactg atgctgggcc tgacgatgct tttcgctgca tttggtctta
    16081 tgagcctgat ggtgctagtt atctgggcgg ttgatccgca atatcgcctg aatgcgatga
    16141 ttgccaccac cgtggtgttg ctgctactgg cactgattgg cggcatctgg acgttacgta
    16201 aatcgcgtaa gtctacgttg ctgcgccaca cgcgccatga gctggcaaac gatcggcagc
    16261 tgctcgagga ggagtcccgt gagcagtaaa gtcgaacgtg aacgacgtaa ggcgcaactg
    16321 cttagccaga tccagcaaca acggctggat ctttccgcca gtcgtcgtga atggctggag
    16381 gcaacaggcg cttacgatcg tcgctggaat atgctgttga gtctgcgctc ctgggcgctg
    16441 gttggcagta gcgtgatggc tatctggacg attcgccatc ctaatatgct ggtccgctgg
    16501 gccagacgcg gttttggtgt atggagcgcc tggcgtctgg tgaaaacaac cctcaagcaa
    16561 caacagcttc gcggttaact tcctctctgg ccggagaaat tccggcctta tccctcaaat
    16621 tttttgaaaa tatttgacag ttttccttgc taacaatcac gcttcgtcac gtttatgatt
    16681 ctctccatcg acagcaacga cgctaatagc gcgcccttgc acaaaaaaac aatcagcagc
    16741 ctgagcggcg cagtggagag tatgatgaaa aaattagaag atgttggtgt actggtagcg
    16801 cgtattttaa tgccgattct gtttattacg gcaggctggg gaaaaattac tggctacgcg
    16861 ggtatccaac aatatatgga agcaatgggc gtccccggct ttatgctgcc gctggtgatt
    16921 ctgcttgagt ttggtggtgg tctggcaatc ctgttcggtt tcctgacacg taccacagcc
    16981 ctgtttactg cgggctttac gctgctgacg gcatttttat ttcacagcaa ctttgcagaa
    17041 ggcatgaact cgctgatgtt tatgaaaaac ctgactattt ctggcggatt cctgctgctg
    17101 gcaattaccg gtccaggcgc attcagcatc gaccgcctgc tgaataaaaa gtggtaagca
    17161 cgcgctatac ttaacgataa aaaacgaggg ggaagctcct cgtttttgct attggaggat
    17221 agaaaaatgg gtcaactgat tgacggcgtc tggcatgaca cctggtacga taccaaatcc
    17281 accggcggta aatttcaacg ttcagcttcc gcatttcgta actggctcac tgccgatggc
    17341 gcgccaggcc ccactggcaa aggcggtttt gccgcagaga aagagcgtta tcatctctat
    17401 gtttcactcg cctgcccgtg ggcacaccgc acgctgatca tgcgtaagct caaaggactg
    17461 gaaccgttta tttccgtttc cgtagtgaac ccgctgatgc tggaaaacgg ctggaccttt
    17521 gatgacagtt ttccgggagc aaccggcgac acactctatc aacatgaatt tctgtatcag
    17581 ctttatctcc acgccgatcc acactacagc ggacgagtga ctgttcccgt gctgtgggac
    17641 aaaaaaaacc acaccatcgt tagcaacgaa tcagcagaaa tcatacgcat gttcaatacc
    17701 gcgtttgatg cgctgggtgc gaaagctgga gattactacc caccagccct gcaaccgaaa
    17761 attgacgaac ttaacggctg gatttacgac accgttaaca atggcgtgta taaagccggt
    17821 tttgccacca gccagcaagc ctacgacgag gcggtggcga aagtgtttga atcgctggcg
    17881 cgactggaac agattttagg tcagcaccgt tacctgaccg gcaaccagct aaccgaagcc
    17941 gatattcgcc tgtggaccac gctggtgcgt tttgatccag tgtatgtgac ccacttcaag
    18001 tgtgataagc accgcatcag cgattacctg aatctgtatg gcttcctgcg cgatatctac
    18061 cagatgccgg gaattgccga aacagtcaat ttcgatcaca tccgtaatca ttacttccgc
    18121 agccataaga ccatcaaccc tacggggatt atttcaattg gtccgtggca ggatctcgat
    18181 gaaccgcatg gacgagatgt tcgcttcggt taaaaaaagg caccttacga ggtgcctctg
    18241 ataattagct aagtacgtgt tgttcatgtc ggatgcggcg taaacgcctt atcaaaccta
    18301 caaaacattt atattcaata tgttgcaatg accgcgcagg ccagataagc gtagcgcatc
    18361 tggcaatttt gcgcttgccc ttatccttca cagcgcctta ttaattcatc gcgataatat
    18421 ttactgccat ttcattcacg tctattctta atttgctgct ttaaaaacaa gtgattgagc
    18481 aaaattgagg caaaaatgga ctggtatctg aaagtactaa aaaattatgt cggtttccgg
    18541 ggtcgtgcgc ggcgcaaaga gtactggatg tttattctgg tcaacatcat ctttacgttc
    18601 gtgctggggc tactggataa aatgttaggc tggcaacgtg ccggtggcga aggcatcctg
    18661 acgacaatct acggtattct ggtgttttta ccatggtggg cagttcagtt ccgccgcctg
    18721 cacgacaccg accgctcggc gtggtgggca ctgctattct taatcccgtt catcggctgg
    18781 cttattatca tcgtctttaa ctgccaggcg ggtacgccag gcgaaaaccg ctttggacca
    18841 gatccgaaac tggaacaaga ataataatta agcaccttgt aaaaggtgct taatcgatta
    18901 ttttccgcta aaaagtttgg gaatttcccg cagacaccag gatttcgcct cccccatact
    18961 gtcacgacgc caggccataa taatatcgat ctcgctggtc gattccgggc tgacgacacg
    19021 caaccgccct tccgcaatat ctttttcgac catcggatac ggcatcgtcg ccacgccaag
    19081 ccccgccagt aatgcctgac gtttatcttc aatcgtgctc accgttaagc gcggctgttt
    19141 gtccagcaac tgtacggtca acaccgggcg ctcacgagcg gtatccgcca ccgcaattcc
    19201 acgatatttc acgcgcgtca cttcagataa cggttccggc tcctgatgaa tcgggtgatc
    19261 cggcgcggca acatagacgt tcattaaggt atagagtttg cgcgagttga tctccgacga
    19321 ggaacgaaaa tgcatatccg gcgcgataac gatatccgcc cgcccctgct ccagccgttc
    19381 ccacgccccc gccagcactt cggtgatgat tgccagttgg gtattggctt ttgccgccag
    19441 tttgtcgatt aacgggaaaa aggcaggtgt cggtaccagc gcctcggtca caatggtgag
    19501 atgcgtttcc caaccacgcg cgagggcttc cgcatcggta gtcagtttat ctgcggcttc
    19561 cagcaaaacg cgccctcgtt ccagcaacat cctcccgaca ttggtgaatt tggtgcgatg
    19621 gcccgagcgg tcaaacagca ccacatccag ctcttcttcc agtttttgca tggtgtagct
    19681 aagtgcggaa ggcacgcgtc ccagctcatc cgccgccgcc gcaaaactgc cccggcgatc
    19741 gatcgcatcc ataacccgta gtgcttccag cgttaatgcc ctttctttgg ccatttcgtt
    19801 ctcattcagg aaatttgaac ataccgggca gaatatctgg ctaacaatgt agcgtccagc
    19861 cccttaccat aaaaggaagt aaagagaggt caagaattat gattactacc cgaactgcca
    19921 ggcagtgtgg acaagcagac tacggatggt tgcaggcccg gtatactttt tcctttggac
    19981 actacttcga cccgaaattg ttaggctatg cctccctgcg cgtgcttaac caggaagtgc
    20041 tggccccagg tgccgccttt cagccgcgaa cctatcccaa agtcgatatt ttaaatgtga
    20101 ttctggatgg agaagctgag tatcgcgaca gcgaaggcaa tcatgttcag gccagcgctg
    20161 gtgaggcgtt gctgctctct acccagccgg gtgtcagcta tagcgaacac aatctcagca
    20221 aagacaaatc gttaacgcga atgcaacttt ggctggatgc ctgcccacaa cgagagaatc
    20281 cgctgattca aaaactagcg cttaatatgg acaagcagca attaatcgcc tcgccagaag
    20341 gtgggatggg aagcctgcaa ttgcgccagc aagtatggct gcatcatatt gtgctcgaca
    20401 aaggcgaaag tgcgaatttc cagttgcatg ggccacgcgc gtatttgcaa tcgattcacg
    20461 ggcaatttca tgcacttacg catcatgaag agaaagcggc gctgacctgc ggtgatgggg
    20521 cgtttattcg tgacgaggct aacattacgc tggttgccga ttccccactg cgcgctttgc
    20581 tgatagattt gcctgtctag ttgtttttac agggagatga tgatgagtaa aaaatcggcc
    20641 aaaaagcgtc agccagtgaa gcccgtggtg gcgaaagaac ctgctcgcac cgccaaaaat
    20701 tttggctatg aagagatgtt gagcgagctg gaagccatcg tcgcggatgc tgaaacgcgt
    20761 ttagccgagg atgaagctac cgcgtaaaga caggtgccgg atgcggcgta aacgccttat
    20821 ccggcacaat acccaaaacc gggcgtttat ctggccttgc tcgccataat ctcgataatc
    20881 tgccgatccg tttgctgcat cgaatggctt gctaacgcac acaggttggc aatcgactgc
    20941 tcaacatcat gcgccacaat cccttcattg ccggtcacgg cggtatcatc cagcgccatt
    21001 aacaccgctt tccacgcagc cgaagcactg gtcgaaacct tcatcgcgca gctgttcgac
    21061 gcaccatcgc aaatcatgcc gctgacatcg ccgatcatac tgctgatcgc catcgagatg
    21121 gtttcataac gcccatccac cagccatgcc atcccggcgg cggcccccat tgctgcggtc
    21181 gttgcggcac acagcgcaga caaacgcggt aactggttat ggatgtaaat cgcgctcaaa
    21241 tgcgaaagca tcagcgcacg cgccagccgt tcatcattcg ctccgaagtg ttctgctacc
    21301 accaccacgg gcattgttgc ggtgatcccc tggttacccg agccggagtt actcattgcc
    21361 ggaagcgtag cgccgcccat acgcgcatcg gatgccgcgc tggtacgaat cacaatggat
    21421 gaagagagat ctttcgccag caaaccgcgc gcgcactgtt tttccagcgt cgcaccaata
    21481 tgcagccccc agttaccgct caaaccttcc tgcgataacg cacaatttaa cttcgcagaa
    21541 tcgagaataa agcggatcgc cgcaaatggg acttcattga cgaacttcag gatctcagcc
    21601 agcgtcgtcc tggaaagcac cgtcagcggc gactcttgct cgccctctgt cacacacgcc
    21661 tgctgggtaa acaccacacc attgtgcgtc tcaatatgca caatgttggt atgcccgccg
    21721 acgatggtga cacacgccca cttcacaccg ttccagactt tggcgcgtga gaagaggatt
    21781 tcatcgcaag gttcctggat cttaactgag actttccccg ccgccagcag tgctttggca
    21841 tcggaaattg cctgcgcagt tgcgtctttc agcacttcca gcccggcgtt agcatttcca
    21901 cctaacgctc ccagcgccgc cgcaattggc agccccacca ttcctgtacc gggaacggtg
    21961 acacccagac cattcttcat cagatttggc gaaacccagg cttctacacg ttcaaccgga
    22021 ccttccagtt cagccgcagc aaccgccgcc gccagcgcca gtgaaatcgg ttcagtacac
    22081 cccagcgccg gttttacttc ctcctgaacg gcgaggatgt aacgctgcca taacggattt
    22141 aaagtcgaat caaacatatt aaaaacctta aaatttcagg taaatcaaga aaatgccagg
    22201 aacggagaaa cacaaagcaa caaaccagtg acgataatca ggtacagaga cattcctttg
    22261 tatttgtgca atgccggtac tttgtaaacc agccacgccg ggatcaggca ccctaccatg
    22321 ccgaaaatcg ggctacagat agaggtgaag ctcaacaccg gggcgttcag tacgatggcg
    22381 ctccaggcca gcaaaatggc gaaaatcatg atgccgcgct gaacgagatt ttcgttaatc
    22441 ttctcggcag gtatcttgcg acgcaggatg ttcattacga tcccttgcgt tgcttcgcga
    22501 aagcctaaat agacgccaaa gaatgcagtc attacagcaa agatattgag aataacgctg
    22561 accactttca cccacgctgc gccgtcaccg ctaataaact gtgcggcaat cgccagcgcg
    22621 gaaatattct gctcgtaggc ttttaccgct tcgtcatgtc ccatcgccag cgtgaatgac
    22681 acggcgtaga aaaagacggt gacaaacaaa atgccaaacg cgatattcat tgcccgcaat
    22741 gctttatgcc gcgccacttc aattgatttt tcccgcgaac gataagagat caccatcggg
    22801 cttaacgtct ggataaacag aatcgacgtc agggtaaacg gcagcgtaat aatggcgttt
    22861 ttcaccagca gccccagcgg cggtagcgaa ccgacgttat acagatgcca cattcccacc
    22921 atcgacacgc ccagcgccgc gaccaccagc agcttggtca gcaccatgcc ggtcgaaatt
    22981 ttgaataaca atttctcgcc gcgtgaggag atcgccacca gaatgcaaat cagtaccaga
    23041 ccataaaagg gactgtctga cagcaaccct tccgtcacgc cgaaggtatg caggtaggaa
    23101 gcactatcgt tggtgatggc ggtggaatag acgaacatcc aaatcaccag cattacgaaa
    23161 tagagtgcac ctaacaggat gccccagttt ttacctaaat aaccgctgat gacgctcggg
    23221 taatctttac actccggtga ttctgccagc gtattaataa acaaccgctg aaacagatac
    23281 attgccgggt aaccaatcac cgatgagagc aaaaataccc acaatcccat caaaccgacc
    23341 tgcaccggga gaaaaacaat ccccgcgcca atcgccatcc cgatactcat aatcacccag
    23401 ccggtgtcag tactgtcgaa tttgatcgct tctcgccact cgctctcact cattccagca
    23461 cgacccgccg gggtcgaagc gtctgcaatg acgcctttat tcgatgcaat ttccataatt
    23521 tctcgctcaa tattttgtag ggcttat
//
LOCUS       DAZFFE010000075        23439 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFFE010000075 DAZFFE010000000
VERSION     DAZFFE010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.075"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..648)
                     /locus_tag="ACHJ69_003147"
     CDS             complement(<1..648)
                     /locus_tag="ACHJ69_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HFO5016080.1"
                     /translation="MSQLDSNAIKEIVKLTTTAFSGENLPLTECPVALLPDNVNIESL
                     ERFMTERFRFRGVMTTTSIDDFVEYSKGYADECSRCFINAETMKAVTVFNIGTLEQPG
                     HADNKALLELKATSPYRALRDVDGKKQLQKSLAEWLEDWADFLTAYDSDGNVLDIKQA
                     ISAVRRLTIDAKRSAEYEEQNFSGSRSVMESVEAKSKEIMPATFRFECIPYEGLGN"
     gene            complement(712..1059)
                     /locus_tag="ACHJ69_003148"
     CDS             complement(712..1059)
                     /locus_tag="ACHJ69_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016081.1"
                     /translation="MNQHQTDVNVFINDLDGGVFVNKLGAVLSEVAFGVNSTNKKGKV
                     CVEFELSSLDENRVSVSHKLKFTRPTMRGSKSEEDTTNTPMFVNKGGELTLFQKDQGQ
                     LFDKQGQHDAVLR"
     gene            complement(1134..1721)
                     /locus_tag="ACHJ69_003149"
     CDS             complement(1134..1721)
                     /locus_tag="ACHJ69_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFO5016082.1"
                     /translation="MDDVIFTYNEESALTAGQGGFITETGAHIINITEAELKQSEKGA
                     RFIEFSGESDDGRKIQYLSVCVQKNDGTENKFGASIIHAMMGCTGIGQLTQHMVSVSK
                     YVAPEFHGKKIGLVLQKVLTTNRKTGADSYQMEIRIPFIAETGQTLKEKAEGKKPETV
                     ANMVSTLKDKDNRSKNANPNHTDDPGYWQYGGDNF"
     gene            complement(1721..2413)
                     /locus_tag="ACHJ69_003150"
     CDS             complement(1721..2413)
                     /locus_tag="ACHJ69_003150"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO5016083.1"
                     /translation="MTPEIILQRTGVDITSLDQGDDGWHKLRLGVITASEVHNVIAKP
                     RSGSKWPDTKMSYFHTLLAEVCTGVAPEVNAKSLAWGKQYEDDARALFEFIADVTVAE
                     TPIIFRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFNAIKPAYMAQVQF
                     SMWVTDKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIDKMDEALAEIG
                     FVFGEQWGGVNN"
     gene            complement(2410..3360)
                     /locus_tag="ACHJ69_003151"
     CDS             complement(2410..3360)
                     /locus_tag="ACHJ69_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFO5016084.1"
                     /translation="MFDIVEFVKQQERFFCEALTEPTLTWAKESQFAIQQFQKNAFLA
                     DTARANLPSAQNAIINVAAIGITLNPASKLAYLVPRKKAVCLDISYMGLLHLAQVTGA
                     IQWGQCKLVYEKDIYESNGIDCAPTHKYNPFVDRGARIGGYCVVKTSEGDYLTEEMSN
                     REIEVIRACSKAGNNGGSSPWDSFPDEMARKAIVKRASKYWPRRDRLDTAIDYLNTQA
                     GEGIILNADHIPERDVTPASDEIINEITQAITEINKKWDDLLPVCSKTFRRTIASHED
                     LTQEEAVKTLDFVKKKAARNKATAEAKVHATTENNSEAVS"
     gene            complement(3377..3658)
                     /gene="gamL"
                     /locus_tag="ACHJ69_003152"
     CDS             complement(3377..3658)
                     /gene="gamL"
                     /locus_tag="ACHJ69_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor GamL"
                     /protein_id="HFO5016085.1"
                     /translation="MNAYLTCDRIEERRWINQQIQDEKDKWIDDRSQELISMFPDKPL
                     LMSSLFLPKEAQLALTGEKAEEAYNDYISAIAYARAEEEWERKFSPCPF"
     gene            complement(3679..3960)
                     /locus_tag="ACHJ69_003153"
     CDS             complement(3679..3960)
                     /locus_tag="ACHJ69_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO5016086.1"
                     /translation="MLNQKININVKSVVTPAGTIMGEVFMDDKIIAYFVVLSDEAISV
                     IDTEGNVMFIAEHPEDIALQAAAYFFAKEQEEECNCPVCQLSRQINLMH"
     gene            complement(3972..4184)
                     /locus_tag="ACHJ69_003154"
     CDS             complement(3972..4184)
                     /locus_tag="ACHJ69_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO5016087.1"
                     /translation="MLKPHYGTALVSREDVKPGTAILYNGRYYMASANVNNALYAHSL
                     IEKIRIISDAIEVYLNNKGQPLISPA"
     gene            complement(4255..4929)
                     /locus_tag="ACHJ69_003155"
     CDS             complement(4255..4929)
                     /locus_tag="ACHJ69_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO5016088.1"
                     /translation="MKTISVESLSIISFSNIPVVTTELLASLYGTEPDYIRKNFNRNS
                     GRFVIGKHYFLLENEELREFKHSMSLRPSVKIARNVRSLILWTERGAARHAKMLETDR
                     AWEVFEKLEDCYFSQKIPEQLQLPESTLSINYPLSWFSDNHPYATMNYVDRKVISLDA
                     SVLFDMPSPTMRILNELHSKGYNVDAAVAEFNAFKHLTEEMRRKLLDISRTSEKSSRF
                     GFNVNL"
     gene            complement(5185..5958)
                     /locus_tag="ACHJ69_003156"
     CDS             complement(5185..5958)
                     /locus_tag="ACHJ69_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO5016089.1"
                     /translation="MGPTSVGLVPLIASSPNPVQSTASELRTSCGGSQSPIKEAAIMA
                     TIPALSQPEITITNGRVVTTSIAIAEFFDKRHDDVLKKIRLMDCSTEFNAHNFADVTY
                     TDAKGEQRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEKQLWEQKQLALSS
                     PAMNINFPLSWFSEHHPYATMNYVDRKVISLDASVLFDMPSPTMRILNELHSKGYNVD
                     AAVAEFNAFKHLTEEMRRTLQDISRLSDRNSRKGFSLSL"
     gene            complement(6587..7540)
                     /locus_tag="ACHJ69_003157"
     CDS             complement(6587..7540)
                     /locus_tag="ACHJ69_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HFO5016090.1"
                     /translation="MNNQNDYIEAAQQIIASLGLPRAQQNERSALCLLALLNLTPGKA
                     WADAENPLVGITPIMNWVREHYGKVYAPNTRETFRRQSMHQFCAAGVALYNPDKPDRP
                     VNSPKAVYQIEPAALSMLRTFGSPAWHDSLATYLAERETLVTRYAKEREQNRIPVEIA
                     AGQQITLSPGEHSELIRAIIEDFAPRFAPGSVLVYAGDTGEKWGYFDAPLLAGLGVDV
                     DSHGKMPDVVLHFTAKNWLLLVESVTSHGPVDGKRHAELARLFAGSTAGLVYVTAFPN
                     RSIMGRYLGEIAWETEVWVADAPSHLIHFNGVRFLGPYSTE"
     gene            complement(7537..9006)
                     /locus_tag="ACHJ69_003158"
     CDS             complement(7537..9006)
                     /locus_tag="ACHJ69_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HFO5016091.1"
                     /translation="MLQKLDVADSVRRELSPRTARKHKAEFGQFMTPSGVARFMASLF
                     PPSTMKTCRLLDAGAGVGALSCAFLDRWLVGEFGFESVEATAYEIDKKLCGHLAKHLT
                     GYRRVTPRIIEGDYIELATAEGLQDRGYTHAILNPPYKKINSQSAHRQALRTVGIETV
                     NLYSAFVALAVGEVAPGGQIVAIIPRSFCNGPYYRPFRNFILERAAIRHIHLFESRSK
                     AFRDDDVLQENIIIRLERGGRQEAVTVTTSTDDSFFDLVTYEHPFDQIVYPDDSERFI
                     HVPTTLEKSTIELSSAVQCSLADIGVKVSTGPIVDFRLKAHLRSMPEEGTVPLIYPSH
                     LSMSSTVWPVEGLKKPNAIMRNDETEKWLYPNGFYCVVRRFSSKEEKRRVVASVVDPA
                     TFSEYSVLGFENHMNVFHENKHGLPEALARGLAMFLNTTAVDKYFRRFNGHTQVNATD
                     LKMIKYPSRDTLIELGKWAMQQETLTQEHIDAKLGALTA"
     gene            complement(9101..9814)
                     /locus_tag="ACHJ69_003159"
     CDS             complement(9101..9814)
                     /locus_tag="ACHJ69_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO5016092.1"
                     /translation="MNMKKKPLTPEQLEDAKRLKSIFNAKKKELGLSQESLAYELGVT
                     QSAVNQLMAGINAINASHAAQLAKILNVKVGDFSPSLAKSIAEMALAIEEPLTRVPAY
                     EYPLLSCVQAGAFSMDDISYTAKDAIKWISTTTKASDRSFWLEVKGHSMTAPQGGKPS
                     FPEGMLILVDPEREIEDGDFCVARMNGDEFTFKRFIRESGKAYLEPLNPRFDMIECNE
                     NCQFVGKVIKSQWNDETFD"
     gene            9910..10113
                     /locus_tag="ACHJ69_003160"
     CDS             9910..10113
                     /locus_tag="ACHJ69_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO5016093.1"
                     /translation="MAMKKETLADYVYRFGQKKAAKDFGVAQSAISKALLVGREIYVK
                     TFDDGTVEAEEVRPFPAFVRGDD"
     gene            10168..10437
                     /locus_tag="ACHJ69_003161"
     CDS             10168..10437
                     /locus_tag="ACHJ69_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016094.1"
                     /translation="MPEQYCDADRDWIQEQLLQLDPTTRVKIAAEYARVYQEEWDKEP
                     VSFRKGNRARRSANTRLRVYVQKYARASRGWMLSPVAVRKFPPTP"
     gene            10645..11022
                     /locus_tag="ACHJ69_003162"
     CDS             10645..11022
                     /locus_tag="ACHJ69_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016095.1"
                     /translation="MSAATKSALIRTLSTVPLRTEERYSFLADVVTILESQGMHVASN
                     VTVRIDGRNFRVDILATAKTGGSVAIEIDRSSPRPRSVMKLRELARRGTEGFVLLRMP
                     KKLTSYSDAGIDIIPANGKGASC"
     gene            11016..12536
                     /locus_tag="ACHJ69_003163"
     CDS             11016..12536
                     /locus_tag="ACHJ69_003163"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO5016096.1"
                     /translation="MLKITPNFAQERGLNQLRHQWKQHRTYLMYAPTGSGKTGLAAFV
                     TAGMVERGMRVMFVCPYTILLNQTAERFTEYGLPWEEISFVWRDHPNYDPSRLIQIAS
                     ADTLIRREFPDNIDLLIIDEAHMKRRALLEVIRDKDIRVLGLSGTPFAAWMGKYYECL
                     IKPTTIRELIQRGDLSDYEFFAPSMPDLAGVKTSNTVFGRDYNEEQLASIMGSSDLVG
                     DIVSNWLENGEDLPTICFCVNVAHANFVTREFLQSGIGAEVMTADTPHDERQDIIRRF
                     EEGATKIIVNVGVLVAGFDSDVRCLIYARPTKSEIRWLQCIGRALRTASGKKRALIFD
                     HSGMVHRLGFPEDIEYDELPGKNDGMKASAGGSEVKAEKLPRECPKCHFMKPAGVHMC
                     PKCGFRPLGGDDVATDRDRKLSRVNKGKREYTREEKQRWWSEIKGYQNYRNATGKPLS
                     DGWCAHTYKEKFGVWPKGFSNAPLQTSVEVYNFIKSKTIAYAKGRKKAMTGGQHAN"
     gene            12526..13497
                     /locus_tag="ACHJ69_003164"
     CDS             12526..13497
                     /locus_tag="ACHJ69_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO5016097.1"
                     /translation="MRTRDAAVGHWSRIFEYYGMPPVTGLKHYSGPCPICGARGKFRC
                     DDKDGSGSWICVCGHGDGMNLLQLATGKPWVTLCDEIDRLIGNTWKREKVSQPVTEIS
                     RKRKLVMDKFAGLPCLRGTTGEAYLQGRGILQLPIESVRFCDRQIASGREYQAIYAIA
                     TDDKGSLCYLHRTLLDGDRKANVEAAKKMTALQELPGLQHAKSVAIRLYPVSSTLGIA
                     EGIETALSCRQIFRCNVWSTMNSGFMEKFIAPPGVNHLIIFADNDAHGAGLAAAFKCG
                     HKNLMSCNDVEKVSIRWPDLPDFNDMLIQGCEAREHVLTRKFKAEAA"
     gene            13497..13946
                     /locus_tag="ACHJ69_003165"
     CDS             13497..13946
                     /locus_tag="ACHJ69_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFO5016098.1"
                     /translation="MEIEMIKAANGVFVPAYERDLPRLAKFKNGELYTLEAKLTRNPS
                     FHRKMFAFLNFCFQYWCAEHAGYEFSDEATQFDEFRKNLTILAGFYDVVTTIRGEVRY
                     RAKSLSYANMDQDEFERCYNAMINAALKHVFGRSNNPELNNRLLSFF"
     gene            13954..14517
                     /locus_tag="ACHJ69_003166"
     CDS             13954..14517
                     /locus_tag="ACHJ69_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO5016099.1"
                     /translation="MKQRKPKKCKVCGSSFVPFRSYQKVCCGQCALELVRKEKAIASA
                     KEQADKLKARRRDLQPRSYWIKQAQQAVNAYIRERERHLPCVSCGTFDSAQWDAGHYR
                     TTAAAPQLRFDERNIHKQCVVCNQYKSGNLVPYRVELINRIGQEAVDEIESNHSRHRW
                     TVEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            14514..14708
                     /locus_tag="ACHJ69_003167"
     CDS             14514..14708
                     /locus_tag="ACHJ69_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO5016100.1"
                     /translation="MTFTVKTIPDMLVEAYGNQTEVARILNCNRATVRKYIGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            14701..15135
                     /locus_tag="ACHJ69_003168"
     CDS             14701..15135
                     /locus_tag="ACHJ69_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO5016101.1"
                     /translation="MRDIRQVLERWGAWAANNHEDVTWSPIAAGFKRLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            15642..16590
                     /gene="stxA1a"
                     /locus_tag="ACHJ69_003169"
                     /pseudo
     CDS             15642..16590
                     /gene="stxA1a"
                     /locus_tag="ACHJ69_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
     gene            16600..16869
                     /gene="stxB1a"
                     /locus_tag="ACHJ69_003170"
     CDS             16600..16869
                     /gene="stxB1a"
                     /locus_tag="ACHJ69_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO5016102.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            17374..19314
                     /locus_tag="ACHJ69_003171"
     CDS             17374..19314
                     /locus_tag="ACHJ69_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5016103.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGELPSLAESGNHPHVSAKPEAAPEYYYVIALAGQSNSMSYGEGLPLPET
                     YDRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKAELSKGQYG
                     TVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDAGGASENSTRWGVD
                     KPLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPVNHAAQFGALVDKFRADLA
                     DMAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDEN
                     GVNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIISDRLATAI
                     LRHAGRVALNVGAPSTVSEVRPSSPSGAEVTGVTTLLSYLASESEGSLKVQGWSASGG
                     RAEVVSDAEGTGGKAVKVTKEAGKSSWVLEYAAGNGAALLQKGGQIRCRFKASGALAA
                     NQYVMAFYWPVSSLPQGVVLTGDGGNNLLAAFYIQTDAKDLNVMYHNAKVATNNLKLG
                     SFGAFDNEWHTLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFSADKLHVTDITK
                     SATYPVLIDSIAVEVNNADAAA"
     gene            19450..19629
                     /locus_tag="ACHJ69_003172"
     CDS             19450..19629
                     /locus_tag="ACHJ69_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO5016104.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMHDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLV"
     gene            19670..19915
                     /locus_tag="ACHJ69_003173"
     CDS             19670..19915
                     /locus_tag="ACHJ69_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO5016105.1"
                     /translation="MSEITSLVTAEAVKDVLRSEEVLSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
     gene            19993..20208
                     /locus_tag="ACHJ69_003174"
     CDS             19993..20208
                     /locus_tag="ACHJ69_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5016106.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            20213..20746
                     /locus_tag="ACHJ69_003175"
     CDS             20213..20746
                     /locus_tag="ACHJ69_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5016107.1"
                     /translation="MSRKFRYGLSAVVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKGKCDQVNAIERDKALAWVAKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRNRSNNCYGQ
                     VSRRDQESALACWSIDR"
     gene            20967..21080
                     /locus_tag="ACHJ69_003176"
     CDS             20967..21080
                     /locus_tag="ACHJ69_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016108.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            21082..21540
                     /locus_tag="ACHJ69_003177"
     CDS             21082..21540
                     /locus_tag="ACHJ69_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO5016109.1"
                     /translation="MNRVLYGVLIVLLLGCGSLWLATDHYRDNAITYKEQRDDAKTKL
                     SQANATIADIQQRQRDVAALDARYTRELADANATIESLRADVSAGRKRLQIAATCAKS
                     TTGASGMGDGESSGLTADAELNYYRLRSGIDKITAQVNYLQEYIRMQCLD"
     gene            complement(21621..22202)
                     /locus_tag="ACHJ69_003178"
     CDS             complement(21621..22202)
                     /locus_tag="ACHJ69_003178"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase"
                     /protein_id="HFO5016110.1"
                     /translation="MLNQTLTKSLLIFLLVIIVSVSIIYIARTPVRLDVNDVTEINQK
                     HPIIFLIRHGERCDRSQRICLSAHEGITVNGANKAQQYGDKFRRMFPYYSLYSTDTLR
                     TMQTATFFSGGKTATIPDISTCDDNAVNNILKISKSDHVTVIFTHNHCLSRIAKKMNG
                     WRFKPDYMGTLVLHRENHNLILDGHLKPNELTQ"
     gene            22805..>23439
                     /locus_tag="ACHJ69_003179"
     CDS             22805..>23439
                     /locus_tag="ACHJ69_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO5016111.1"
                     /translation="MAKLDWKKLEQAFRREHVKTGIKLQDWCRQNNISYDTARRYIKL
                     RKHSPRNTEKSAQKNAQIDDQKSAQKSDGNSSHDEPSGDDGCDEKCANLAETKRTRGS
                     RLLPPSNAFSQRNTHAVRHRGYAKYLEADNLIDDAVGMELADELVFTRARALSVTGTL
                     KKMFADLKEAADVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLA"
BASE COUNT         5918 a         5238 c         6161 g         6122 t
ORIGIN      
        1 gttgcccagg ccctcgtatg ggatgcattc aaagcggaaa gtggcaggca tgatttcttt
       61 gcttttagcc tcaacggact ccatgactga gcggctaccg ctgaagtttt gctcttcata
      121 ttcagcactg cgttttgcgt cgatggtaag acgacggaca gcagagatcg cctgtttgat
      181 atccagcaca ttaccgtcgc tgtcataggc tgtgaggaag tcggcccagt cttccagcca
      241 ttcagcgaga gatttctgga gttgtttttt gccgtccacg tcacgtaatg cgcgatatgg
      301 tgatgtggct ttcagttcca gcagtgcctt gttgtcagcg tgtccaggtt gctccagagt
      361 gccgatattg aacacagtga ctgctttcat cgtttccgcg ttaatgaagc agcgggagca
      421 ctcatcggca taccctttgc tgtattcaac aaagtcatca atactggttg tggtcatgac
      481 tccacggaaa cgaaaacgtt ctgtcatgaa tcgttccaga ctttcgatat ttacattgtc
      541 cggcaataac gcaacagggc attccgtaag cggcaaattt tcaccagaaa aagcagtagt
      601 ggtgagtttt acaatttctt taatggcatt gctatctaac tgagacatgt atatttacct
      661 ttaatttatg tatttaggga taatgcgcga gcgttttaag gcggggatta ttcagcgtaa
      721 aacagcgtca tgttggccct gtttatcaaa aagctgtccc tggtctttct ggaacaaagt
      781 aagctcacca cctttattta caaacattgg tgtgttagtc gtatcttctt ctgatttact
      841 accacgcatt gtcgggcgtg tgaattttag tttatgggaa actgatacgc gattttcatc
      901 aagtgaagat aattcgaatt caacacatac ctttcctttt ttgtttgtgc tgtttacgcc
      961 aaaggcaact tcacttaata ccgcaccaag tttgttaaca aataccccgc cgtcgaggtc
     1021 gttaatgaaa acattaacat cagtttgatg ctggttcata ttaatatctc cgtgtagagg
     1081 tgtgttaagg cttcagttgt tcactgcatt taaagcctgg ttttgtttgt gaattaaaaa
     1141 ttatcgccac cgtactgcca gtaacccggg tcgtccgtgt gattcgggtt tgcgtttttg
     1201 ctgcgattgt ctttgtcttt gagagtggaa accatatttg ctaccgtttc tggtttcttt
     1261 ccttcagctt tttctttcag tgtctgccct gtttctgcaa tgaatggaat gcggatttcc
     1321 atctggtaac tgtctgcgcc agtctttctg tttgtggtta atactttctg gagcactaac
     1381 ccgattttct ttccgtgaaa ctcaggtgca acatatttac tgacggaaac catatgttgc
     1441 gttaactgcc caattcctgt gcatcccatc atggcgtgaa taatgctcgc cccaaattta
     1501 ttttcagtgc cgtcattctt ctgaacacaa acactgagat attggatttt ccgtccgtcg
     1561 tcggattctc cagaaaactc aatgaatcgg gcaccttttt ctgattgctt gagttctgct
     1621 tcggtgatgt taatgatatg cgcacccgtt tcggtaataa aaccaccttg tccggcggtt
     1681 agtgctgatt cttcattgta agtaaaaatt acatcatcca ttagttatta accccccccc
     1741 attgttctcc aaatacaaaa ccaatttccg ccagtgcttc gtccattttg tcgatgaact
     1801 ccggcaccat ctcatcaaaa ctcgccatgt acttttcatc ccgctcgacc acgacataat
     1861 gcaggccttc acgcttcata cgtgggtcgt agttggcgaa gtaccaggcg tccttgtctg
     1921 taacccacat gctgaattgc acctgggcca tgtaagcagg cttgatagcg ttaaagccgc
     1981 caagccggaa cttcatgaag tcgcgggaag tgaaggggca tttaagctca agaccgttac
     2041 cgtcgctgca taaaccgtcg ggagagcagg cggtgcgcat gctttcgtca cgaaaaatta
     2101 ttggcgtttc cgcgacggta acatccgcga taaattcgaa gagggcacgg gcatcatctt
     2161 cgtattgctt tccccatgcg agcgacttag cgttaacttc cggtgccacg ccagtacaaa
     2221 cttcagccag cagggtgtgg aagtatgaca tttttgtatc aggccatttg ctgcctgaac
     2281 gtggtttggc tatgacgttg tgaacttctg aagcagtgat aacaccgagt ctcagcttgt
     2341 gccatccatc atcgccctgg tcgaggctgg taatatccac gccagttcgc tggagaataa
     2401 tttctggtgt catgacacgg cctcgctgtt attttccgtg gtggcgtgaa ctttcgcttc
     2461 cgccgtggcc ttgtttctgg cagctttctt tttgacaaaa tccagcgttt tgacggcttc
     2521 ttcctgcgtg agatcttcat gtgatgcaat cgtgcgacgg aatgttttgg aacatacggg
     2581 gagaaggtca tcccatttct tgttgatttc ggtgattgcc tgagtgatct cattgataat
     2641 ctcatctgat gcgggagtga cgtcgcgctc agggatgtga tcagcattca ggataatacc
     2701 ttcaccggcc tgagtgttca ggtagtcgat agctgtatcc aggcgatcgc gacggggcca
     2761 gtatttgctg gcgcgtttaa caatggcttt gcgggccatt tcatccggga agctatccca
     2821 cgggctactt cctccgttat ttccggcttt gctgcacgcc ctgatgacct cgatttccct
     2881 gttgctcatc tcttcggtca gatagtcgcc ttcggatgtt tttacgacac aataaccgcc
     2941 aatgcgtgcg cccctgtcta cgaatgggtt gtatttgtgc gtgggggcgc agtcaatacc
     3001 gttggactcg taaatgtcct tctcgtaaac aagtttgcat tgcccccact gaatggctcc
     3061 tgtgacctgt gccagatgca gaagccccat ataactgata tccaggcata cagccttttt
     3121 tcgtgggacc agatacgcca gcttgctggc cgggttcagg gttatgccga tggcggcaac
     3181 attgatgata gcgttctgtg cgctgggcag atttgcccgt gctgtgtcag ccagaaaggc
     3241 atttttctgg aattgctgaa ttgcaaactg actttccttc gcccatgtca gcgtcggttc
     3301 agttaatgcc tcgcagaaaa agcgctcctg ctgcttaaca aattcaacga tatcgaacat
     3361 tttttggtcc tgaaaatcag aaaggacagg gggagaattt tctctcccat tcttcttccg
     3421 cccgagcata ggcgatcgct gagatataat cgttgtacgc ctcttcagct ttttcgccag
     3481 tgagtgccag ttgggcttct ttgggtaaaa aaaggctgct cataagcaat ggtttatcgg
     3541 ggaacatgct gataagctcc tgcgaccgat catcaatcca tttatccttt tcatcctgaa
     3601 tttgctgatt aatccagcga cgctcctcta tgcggtcgca ggtgaggtat gcgttcatgg
     3661 cggaactcct gattccggtt aatgcattaa attaatttgt cgggaaagct gacatacagg
     3721 gcagttacat tcttcctcct gctccttagc gaagaaatat gcagcggcct gtaatgcgat
     3781 gtcttctgga tgttctgcga taaacataac attgccttcc gtatcaataa cagaaatagc
     3841 ctcatcagac aggacgacaa aataggcgat gattttatca tccataaaaa cttctcccat
     3901 tatcgttcct gctggagtta cgacgctttt tacattgata tttatttttt gattgagcat
     3961 gatatttcct ttcaggctgg tgagattaac ggttggcctt tattgttcag gtaaacttct
     4021 attgcatctg agataatgcg aattttttca atcagtgaat gagcgtaaag tgcattatta
     4081 acgttcgctg acgccatgta ataacgcccg ttgtaaagaa ttgctgtgcc gggtttaacg
     4141 tcctcgcgag aaactaatgc ggttccgtag tgaggtttga gcatgacaaa tcctccggtt
     4201 aattaatcca gatatttaat ttaatccccg atatgtggtc ggggatgggg ttaattaaag
     4261 attaacgttg aaaccaaagc gggaagactt ttccgatgtg cgggaaatat ccagcaattt
     4321 acggcgcatt tcttccgtca gatgtttgaa ggcgttaaat tcagcgacag ctgcgtcgac
     4381 gttatagccc ttgctgtgta gctcgttgag gatgcgcata gttgggcttg gcatatcgaa
     4441 gagcacggaa gcatcaaggc tgataacctt gcgatcaaca tagttcatcg tggcgtaggg
     4501 atggttatct gagaaccacg agagagggta gttgatagat agcgttgatt ctggaagctg
     4561 aagttgttct gggatttttt gactgaaata gcagtcttcc agtttttcga acacttccca
     4621 cgcccgatcg gtttcgagca tttttgcgtg acgggctgcg ccgcgttctg tccagaggat
     4681 gagggagcga acgttacggg cgattttcac agaaggtctt aaagacatgc tgtgcttaaa
     4741 ttcccgcaac tcctcattct caagtaaaaa gtagtgctta ccgataacaa atcgtccaga
     4801 attccgattg aaattttttc ggatgtaatc tggttctgtg ccatataagc tggcaagaag
     4861 ctctgtagtc accacaggta tattactaaa tgaaattatg gagagggact ccacggagat
     4921 ggttttcatg ctttgcgtcc tacttgatat tttgatttac cccttttgag agggtgaccg
     4981 ggcgctcaaa accgcaagta gtcggcgggc atattcccat tgctggtatt gtattagccg
     5041 cacgcccggt cataaaccaa gaagactgga cacaaaaaaa ccgcatgtct gtcgggcgcg
     5101 gtaaccgcta cttgctaagg tgttttgagc accatgaggc agaatatatc cccgttaatg
     5161 cagcattgtc aaacgatctg ttctttaaag acttaacgag aagccttttc gggaatttcg
     5221 atctgacagt cttgaaatat cctgtagcgt gcggcgcatt tcttccgtca gatgcttgaa
     5281 ggcgttaaat tcggcgacag ctgcatcgac gttatagccc ttgctgtgta gctcgttgag
     5341 gatgcgcata gttgggcttg gcatatcgaa gagcacggaa gcatcaaggc tgataacctt
     5401 gcgatcaaca tagttcatcg tggcgtaggg gtgatgctct gaaaaccacg acaaagggaa
     5461 attgatattc atcgcaggag aagacagggc cagttgtttc tgttcccata gttgtttttc
     5521 catgcgatcg aattcagcaa tgtaggcttc cttgaatgca gctgcttttt tgccggtgaa
     5581 gcccatcacc agaaaaacga agccgttttt ggtgatttgg tacattgggc gttgttcgcc
     5641 tttggcgtcg gtgtaggtaa cgtccgcaaa attgtgggcg ttaaattccg tagaacaatc
     5701 catcaatcgg atttttttta aaacgtcgtc gtgccgcttg tcaaaaaatt cagcaatagc
     5761 aatagacgtt gtgacaacgc gaccattggt aatggtgatt tcaggttgag aaagggcagg
     5821 gatagtagcc ataatggcag cctccttgat tggtgattga gaaccaccgc aggaggtcct
     5881 aagctcgctg gcggtggact gtacagggtt aggactactg gcaatcaagg gaaccagccc
     5941 gaccgaagtc ggccccatac agcccaccat tgataagatg tgcgtgtatg tcgatacaaa
     6001 aaaagacgct ggcgcgtctg tatcgcctcg attgtcagcg gggtcctaat cccgacaccc
     6061 gttttataag gtgttcgcca aatatagccc cgacatcaca cgcagtcaat accgtccttt
     6121 ctcggaaatg ctttggcggt gccgcctgca ccccaaaaca ttccctgtat tggtcagcgc
     6181 caacttcctg ccagtgttgc ccgttctcac gccgttctcg ctctcgcgcg gggatactct
     6241 ctcatcgacc ggatcgcacc cgatgataca gcacgtttac gtgtaggggt ctaaacaggt
     6301 cattgacgct gtaaagctcc agattgttaa agagcatttt gcggcgggtt aagtcgcgcc
     6361 gtacgactga tttatgtagc cctgtgtaag ggcgcgatgt ttctggcttg aaataaatat
     6421 aacttgcggt gattttcatg taaataccgt tggtgctttt taagggcgag aaattattac
     6481 ttaactgatt tttaaagtga tttatttttt aagggggcag ataacaggag gggatatgca
     6541 aagaaaaacc cggcgcatgg gccgggctat ttatctggtc ctgttctcat tcagtagagt
     6601 aggggccaag aaatcgcact ccgttgaagt gaataagatg agaaggtgca tcagccaccc
     6661 atacctctgt ttcccatgcg atttcaccaa gatatcgccc catgatggag cgatttggaa
     6721 aagcggtcac atagacaagt ccggctgttg atccggcaaa cagcctggca agctcagcat
     6781 gccgcttccc atcaaccggt ccatgactgg tgacagactc aaccagtagc agccagtttt
     6841 tcgcagtaaa atgtagcacc acatctggca ttttaccgtg tgaatccaca tcaacaccta
     6901 gcccggccag cagtggagcg tcgaagtagc cccacttttc gccagtgtcg ccagcataga
     6961 ccagcacgct acccggagca aagcgtgggg cgaagtcctc tattatggca cggataagtt
     7021 cgctgtgctc gccgggactg agggttattt gctggcccgc agcaatttca acagggatac
     7081 gattctgttc gcgctcctta gcatagcggg taaccagcgt ttcacgctcg gctaaatagg
     7141 ttgcaaggct atcgtgccat gccgggctgc cgaaagtgcg tagcatggac agagcagcag
     7201 gttcgatctg ataaacagcc ttcgggctgt tcactgggcg atcgggcttg tccggattgt
     7261 agagggctac tccagcagcg cagaactgat gcatagactg gcggcggaat gtctcacgag
     7321 tattgggtgc gtagaccttg ccgtagtgct cccgaaccca attcatgatt ggtgtaatgc
     7381 ccacaagcgg attttccgcg tcggcccatg cttttcccgg tgtgaggttc aggagtgcca
     7441 gcaaacacaa agcagaacgc tcattctgct gcgctctggg caggcctaag gatgctataa
     7501 tttgctgagc ggcctcaata taatcatttt ggttgttcat gcagtcagag ctcctagctt
     7561 agcgtcaata tgttcttgcg tgagagtttc ttgttgcata gcccacttgc caagctctat
     7621 cagagtgtca cggcttgggt acttaatcat cttgaggtcg gttgcattga cctgtgtatg
     7681 cccgttgaag cgacggaaat acttatccac tgcggtagtg ttcaggaaca tggccaagcc
     7741 acgggccagc gcctcgggta acccatgttt gttctcatga aacacgttca tgtgattttc
     7801 aaatcccaac actgaatact cactgaaggt agcaggatca acgacgcttg ctaccactcg
     7861 gcgcttttcc tccttcgacg agaaacgacg caccacacaa taaaaaccgt tcgggtaaag
     7921 ccacttttct gtctcgtcgt tgcgcatgat tgcgttgggt ttcttcaaac cttccacagg
     7981 ccagacagtg ctactcatac tcaaatggct cgggtagatc aggggaacag ttccttcttc
     8041 tggcatgctg cgcaaatgag ctttcagtcg gaaatcaact atcggtccgg tcgatacctt
     8101 cacgccaata tcagccagcg aacattgtac ggcagatgat aactcaatgg tgcttttctc
     8161 gagcgtggtc ggcacatgaa taaaccgctc cgaatcgtcc gggtacacaa tctggtcaaa
     8221 tgggtgttcg taggtgacta ggtcaaaaaa gctatcatcg gtcgaagttg tgactgtaac
     8281 agcctcctga cggcctccac gctccaggcg gatgataata ttttcctgca acacatcgtc
     8341 atccctgaaa gctttgctac gtgattcgaa caaatgaata tggcggattg ctgcccgctc
     8401 aagtatgaaa ttacggaacg gacggtaata cggtccgttg cagaaactac gtgggattat
     8461 ggccactatc tgcccgccag gtgcaacctc acctacagcc agtgcaacaa aggcggaata
     8521 cagatttacc gtctcgatac cgacagtgcg aagagcctgc cggtgggctg actggctatt
     8581 gatcttcttg taaggcggat taagtattgc atgggtataa cctcgatcct gtagaccttc
     8641 ggcggttgcc agctcaatat aatccccttc gatgatgcgg ggcgtcactc tcctgtaccc
     8701 ggttaagtgc ttcgcaaggt gaccacacag ctttttgtcg atttcgtagg ctgttgcctc
     8761 gacagactca aagccaaact cgccaaccag ccaacggtcg aggaaggcgc aagacagtgc
     8821 gcctacacca gcgcctgcgt ccagtagacg gcaagttttc attgtgctcg gtggaaacaa
     8881 ggaggccata aaacgggcca cgccagaagg cgtcatgaac tggccgaact ctgctttgtg
     8941 tttacgcgcg gtacggggag acagctcgcg acgcacgctg tctgcgacgt ctagcttttg
     9001 aagcataact aatcctcttt acgtggcatg atgatgccag cgtgcttttg acatcctcca
     9061 cattcttcag gcgtgaagga tgtcaaatca ctctgtgagc tcaatcaaaa gtctcatcat
     9121 tccactgcga tttgatgacc tttccgacaa actggcagtt ttcgttacac tcaatcatgt
     9181 cgaatcgtgg gttgagcggc tccaggtacg ctttaccgct ctcacgaatg aatcgtttga
     9241 aggtaaattc atcgccgttc attcgggcca cgcaaaaatc gccatcctcg atttcccgtt
     9301 cagggtcaac gagtatcagc ataccttcag ggaagctggg tttaccaccc tgcggtgcgg
     9361 tcattgaatg ccccttgacc tccagccaga acgacctgtc actggctttt gtggtggtgg
     9421 agatccattt aatcgcatct tttgcggtgt acgaaatatc atccattgag aatgcgccag
     9481 cttgtacgca ggagagtaac ggatattcgt aagcggggac acgagttaat ggctcttcaa
     9541 ttgcgagcgc catttctgca atagattttg ccaggctggg actgaaatct ccgaccttta
     9601 cattcagtat cttagcaagc tgtgctgcgt ggctggcatt tatggcgttg atgccagcca
     9661 ttagctgatt aacagcgctt tgggtaacac ctaattcata ggctaatgat tcctgcgaca
     9721 ggcctaactc tttcttttta gcgttaaaaa tacttttcag acgcttggcg tcttccagtt
     9781 gctcaggggt taacggtttc tttttcatgt tcataccata tcacctttgg ttatatgtaa
     9841 tgaaatatct gcggtgttga cattttaata actgtgcgta atgatatggg cattttgaag
     9901 gagtcagtta tggcgatgaa aaaagagacc ctggctgatt acgtatacag gttcggccaa
     9961 aagaaagccg ctaaggactt tggcgtggcg cagagtgcaa tcagtaaagc gcttctcgtc
    10021 ggacgagaaa tatacgtcaa aacctttgat gacggcacag ttgaggcgga agaggttcgt
    10081 ccgttccctg cgttcgtaag aggcgatgat taatcctgga cacacagccc atcggaggtg
    10141 tgagtgaaca ggcaaatcaa aacggttatg cccgagcagt actgtgatgc tgatcgggac
    10201 tggatacagg agcaattgtt acagctagac ccgaccaccc gggtaaaaat tgcagcagag
    10261 tacgcgagag tgtaccagga agagtgggac aaggagcctg tatcgttcag gaagggcaac
    10321 agggcaagac ggagcgccaa cacccggtta cgggtgtatg tccagaagta cgcgagagcc
    10381 agccgtggct ggatgctttc gccagtagcg gtacggaagt ttcccccgac accatgatgg
    10441 cggttagttg cgctagagat aatttcaagt gcaactaatt tcctgaaagg aagcgaggag
    10501 ggggtaaggg ggtggagtcg ttggtaaggc ctgtatccct tggcctaggc cagaaggcag
    10561 gtacataggt taggtaggtc actgtataag cgcgcgatag tttctggtga aatgattatc
    10621 gagctaacta ttgagtgata aactatgtcg gcagcaacca aatcggcact gatccgtaca
    10681 ctgagcactg taccactgag aacagaggag cgttacagct ttctggctga tgttgtaacg
    10741 atccttgagt cacaggggat gcatgtggca agcaatgtca cagtaagaat cgatggcaga
    10801 aatttccgtg ttgatattct cgcaacagca aaaactggtg ggagtgtggc tatcgagatc
    10861 gatcgtagct ctccgcgacc ccgctcagtc atgaaattgc gtgaattggc acgtcgtggc
    10921 actgaaggct ttgtgttgct tcgaatgcca aagaaactga ccagttatag cgatgctggg
    10981 atcgatatca tcccggcgaa tggcaagggg gcgtcatgct gaaaatcacc ccaaactttg
    11041 ctcaggagcg cggtttaaac cagctgcggc accagtggaa acagcatcgc acgtacctga
    11101 tgtatgcacc cactggctct ggcaaaactg gtcttgcagc attcgtcact gccggaatgg
    11161 ttgaacgtgg tatgcgggtg atgtttgtct gtccgtacac catcctgctg aaccagacag
    11221 cggaacgttt caccgaatac ggtttgccgt gggaagaaat cagttttgta tggcgtgatc
    11281 accctaacta tgacccgtca cgactgattc agattgccag tgccgacacg ctgatccgtc
    11341 gtgagtttcc tgacaacatc gatctgctga tcatcgatga agcacacatg aagcgtcgtg
    11401 cgttacttga ggttatccgg gacaaagata tcagggttct tgggctttcc ggcaccccgt
    11461 tcgcagcctg gatggggaag tactacgaat gcctgatcaa gcctacaacg attcgtgaac
    11521 ttattcagcg tggtgatctg agcgattatg agttttttgc cccctcaatg cctgatctgg
    11581 ctggcgtaaa aaccagtaac accgtatttg gacgggatta caacgaggaa cagctcgcat
    11641 ccatcatggg gagttcggat ctggttggcg atatcgtcag caactggctt gagaatggcg
    11701 aggatttacc gacgatctgc ttctgcgtga atgtggctca tgcgaacttt gttacccgtg
    11761 agtttttaca gtctggcatt ggcgcagagg tgatgacggc agataccccc catgatgaac
    11821 gacaggacat cattcgccgt tttgaagagg gtgcgaccaa aataattgtc aacgttggcg
    11881 tactggttgc gggatttgac agcgatgttc gctgcctgat ctacgcccgt ccgaccaaat
    11941 cagaaatccg ctggttgcag tgcatcggga gagcgttacg tactgcgtct ggtaaaaaac
    12001 gcgcattgat tttcgaccat tccggcatgg ttcatcggct gggcttcccc gaggatatcg
    12061 agtatgacga acttccggga aaaaatgacg gaatgaaagc gtctgctggc gggagtgagg
    12121 ttaaggctga gaaacttccc agggaatgcc ccaaatgcca cttcatgaag cctgctggcg
    12181 tgcatatgtg tcccaaatgt ggatttcgtc ctcttggtgg tgatgacgtg gcgacagatc
    12241 gcgatcgtaa gctttcacgc gtcaacaaag ggaaacgcga atacacccgt gaggaaaaac
    12301 agcgttggtg gagcgagatc aaggggtatc agaattaccg caacgcgaca ggtaagccac
    12361 tgagtgacgg atggtgtgct catacctaca aggagaagtt cggggtttgg cctaaaggct
    12421 tcagtaatgc gccgctgcaa acctcagttg aagtgtacaa cttcatcaag tcaaagacca
    12481 ttgcctatgc caaagggcgc aagaaagcca tgacaggagg ccagcatgcg aactagagat
    12541 gcagccgtgg ggcactggag ccggattttt gaatattacg gcatgcctcc tgttactggc
    12601 ttgaaacatt atagcggccc ctgcccgata tgtggtgcca gaggtaaatt tcgctgtgat
    12661 gataaggatg gttccggttc atggatttgt gtctgtggtc acggggacgg gatgaatctg
    12721 ttgcaacttg ccacgggtaa gccctgggtg acgttgtgtg atgaaatcga tcggctgatc
    12781 ggaaatacct ggaagaggga gaaagtcagc cagcctgtaa cagagataag cagaaagcgg
    12841 aagctggtca tggataagtt tgccggactc ccgtgtctgc ggggcacaac aggtgaggcg
    12901 tacctgcagg ggaggggaat actccagtta ccgatcgaat ccgtgcgttt ctgtgaccgt
    12961 cagatcgcca gcgggcgcga atatcaggca atttacgcca ttgcaacaga tgacaaaggt
    13021 tctctttgct atctgcatcg tacgttgctg gatggtgatc gcaaggcgaa tgtagaggcg
    13081 gctaaaaaaa tgactgcgct acaggagttg cctggtttgc agcatgctaa atcggtggca
    13141 atacgcctgt atccggtgtc gtccactctg gggatagccg agggtatcga aacggcgctt
    13201 tcatgccgtc aaatcttccg ctgcaatgtg tggtcaacaa tgaactccgg ttttatggag
    13261 aagttcattg cgccacctgg cgttaatcac ctgattatct ttgctgacaa tgatgcgcac
    13321 ggcgcaggtc tggcggctgc ctttaaatgt gggcataaga atctcatgag ttgcaatgac
    13381 gttgagaaag tcagcattcg ctggcctgac ttgccggatt ttaacgacat gctcattcag
    13441 gggtgtgaag cccgtgaaca tgtgttgacg cgcaaattca aagcggaggc tgcctgatgg
    13501 aaatagagat gatcaaggcg gctaatggcg tatttgtacc ggcgtatgag cgcgatttac
    13561 cccgactggc aaaatttaaa aacggtgagc tgtatacact ggaagcaaaa cttacccgta
    13621 acccatcttt tcaccggaag atgttcgctt ttcttaattt ctgctttcag tactggtgcg
    13681 ctgaacatgc tggatacgaa ttctctgatg aagcgacgca gtttgatgag tttcgtaaaa
    13741 atctgacaat tcttgccggg ttctatgatg tggtcacaac cataagaggc gaggtgagat
    13801 atcgggcaaa aagcctgagt tacgcgaata tggatcagga tgagtttgaa cgttgctaca
    13861 acgcaatgat taatgccgcg ttaaaacatg tgttcgggcg ctcaaacaat cctgaactga
    13921 ataaccgcct gctgtcgttt ttctgaggtg atgatgaagc aacgtaaacc aaaaaaatgc
    13981 aaagtgtgcg gctcctcgtt tgtgccgttc cgctcatatc agaaagtttg ctgtggtcag
    14041 tgcgcactgg aactggtcag aaaagaaaag gcgatagctt cagcaaaaga gcaggcagac
    14101 aagctgaaag cgcgcaggag ggacttacag ccccgcagtt actggattaa gcaggcacaa
    14161 caggctgtga atgcttatat cagggagcgg gagcgtcatt tgccgtgtgt ttcatgcggg
    14221 acgttcgatt cagcccagtg ggatgcaggc cattaccgta caacagctgc ggcacctcag
    14281 ctcagatttg atgaacgcaa tatccataag caatgcgtgg tgtgtaacca gtacaaaagc
    14341 ggaaatctcg ttccgtatcg tgtcgaactg attaaccgca tcgggcagga agcagtagac
    14401 gaaatcgaat caaaccatag tcgccaccgc tggactgtcg aagagtgcaa ggcgatcaag
    14461 gcagagtatc aacagaaact taaagacctg cgaaacagca gaagtgaggc cgcatgacgt
    14521 tcaccgtaaa aaccattcct gacatgcttg ttgaggcata tggaaatcag accgaagtgg
    14581 cccgaatact gaactgcaat cgtgccacag tcagaaaata cattggcgat aaagaaggga
    14641 aaagacacgc tatcgtcaac ggtgttctta tggttcaccg cggatggggt aaagatactg
    14701 atgcgtgata tccggcaggt tcttgagcgc tggggggcat gggcggcaaa taaccatgag
    14761 gatgttacat ggtcgcccat tgctgccgga tttaagaggc tgatccccga aaaagtaaaa
    14821 tcacgtccac agtgttgtga cgatgacgcg atgattatat gcgggtgtat ggctcgcctt
    14881 aacagaaaca acagcgatct gcatgacttg ctggttgatt attacgtgtt gggggagacg
    14941 ttcatggcgc tggcacggaa acatgggtgc tctgacacct gtataggtaa acgccttcac
    15001 aaagcggagg ggattgttga aggcatgctg atgatgctgg gagtgaggct tgagatggat
    15061 cggtatgttg agcgtgaatt gccgggaggg agaacctctg tattttatca gcgaaaaaat
    15121 agtttacgat cgtaaaaatc tgcatatcat gataagagtg gttacattgc cacgctgctt
    15181 aacccgccga tgcgcgggtt tttttgtacc cagaatcctg tgagctatac ggaaagtaca
    15241 cagaaaggaa ggtgcgacca caattaataa caaaatctta aaaatcgcac atggcactat
    15301 tagttttcta aatattgtgt attttttgta ttgcaggatg accctgtaac gaagtttgcg
    15361 taacagcatt ttgctctacg agtttgccag cctcccccag tggctggctt ttttatgtcc
    15421 gtaacatcct gtgtatcaat aaatgttgtt gtctacgtac gtcaagtagc cgcatgagat
    15481 ctgaccagat atgttaaggt tgcagctctc tttgaatata attatcattt tcattacgtt
    15541 attgttacgt ttatccggtg cgccgtaaaa cgccgtcctt cagggggtgg aggatgtcaa
    15601 gaatatagtt atcgtatggt gctcaaggag tattgtgtaa tatgaaaata attattttta
    15661 gagtgctaac ttttttcttt gttatctttt cagttaatgt ggttgcgaag gaatttacct
    15721 tagacttctc gactgcaaag acgtatgtag attcgctgaa tgtcattcgc tctgcaatag
    15781 gtactccatt acagactatt tcatcaggag gtacgtcttt actgatgatt gatagtggca
    15841 caggggataa tttgtttgca gttgatgtca gagggataga tccagaggaa gggcggttta
    15901 ataatctacg gcttattgtt gaacgaaata atttatatgt gacaggattt gttaacagga
    15961 caaataatgt ttttttatcg ctttgctgat ttttcacatg ttacctttcc aggtacaaca
    16021 gcggttacat tgtctggtga cagtagctat accacgttac agcgtgttgc agggatcagt
    16081 cgtacgggga tgcagataaa tcgccattcg ttgactactt cttatctgga tttaatgtcg
    16141 catagtggaa cctcactgac gcagtctgtg gcaagagcga tgttacggtt tgttactgtg
    16201 acagctgaag ctttacgttt tcggcaaata cagaggggat ttcgtacaac actggatgat
    16261 ctcagtgggc gttcttatgt aatgactgct gaagatgttg atcttacatt gaactgggga
    16321 aggttgagta gtgtcctgcc tgactatcat ggacaagact ctgttcgtgt aggaagaatt
    16381 tcttttggaa gcattaatgc aattctggga agcgtggcat taatactgaa ttgtcatcat
    16441 catgcatcgc gagttgccag aatggcatct gatgagtttc cttctatgtg tccggcagat
    16501 ggaagagtcc gtgggattac gcacaataaa atattgtggg attcatccac tctgggggca
    16561 attctgatgc gcagaactat tagcagttga gggggtaaaa tgaaaaaaac attattaata
    16621 gctgcatcgc tttcattttt ttcagcaagt gcgctggcga cgcctgattg tgtaactgga
    16681 aaggtggagt atacaaaata taatgatgac gataccttta cagttaaagt gggtgataaa
    16741 gaattattta ccaacagatg gaatcttcag tctcttcttc tcagtgcgca aattacgggg
    16801 atgactgtaa ccattaaaac taatgcctgt cataatggag ggggattcag cgaagttatt
    16861 tttcgttgac tcagaatagc tcagtgaaaa tagcaggcgg agattcataa atgttaaata
    16921 catctcaatt cagtcagttg ttgccggtct gataatagat gtgttagaaa atttctgcat
    16981 ggtgaatccc cctgtgcgga ggggcgactg gtgaacggta tgatctcttt gatgatcgta
    17041 agcgagaata cgcgggtttg gtggcaccag accgaactca ccgggaggca cccggcacca
    17101 tgcagtattc agagattagg catatatcca ggctcctcat cgcaggagcc tttttacatg
    17161 caaaaaaaaa ccgctcctgg gaagagcggc tggcaagaag aaacaatatg aacaattaat
    17221 taacgatggg aataatacct tagagtaatc accttgcgca actgtaaggg catatttcat
    17281 ctttgcgggc tgtttttctg tgtggcttct gtgtttccgg agggcagcct gtaccttttc
    17341 tgactcagaa cattatcccg gccgggagga ttcatggcat ttaaacacta cgatgtggtc
    17401 agggcggcat cgccgtcaga ccttgctgaa cgaataactc aaaaactgaa ggaagggtgg
    17461 cagccttatg gtagtgcgct gatttcgaca gctggttatg gtgcagagtt tatccagcca
    17521 gttgtgagtg agggggagct tccctcactt gcagagtcag gcaatcaccc gcatgtttca
    17581 gcgaagcctg aagcagcgcc ggaatattac tatgtgattg cgcttgccgg tcagtccaat
    17641 agcatgtcat acggtgaagg tcttccgttg ccggagacat atgaccgccc ggacccgcgt
    17701 attaagcagc tggcgcgccg cagtacggtg acaccgggcg gtgcagcatg caaatataac
    17761 gacatcattc cggcggacca ttgtctgcat gatgtgcagg acatgagccg tcttaaccat
    17821 ccgaaagcgg aactgtcaaa ggggcagtac ggaaccgtgg ggcaggggct gcatattgcc
    17881 aaaaaattgc tgccgtttat accggcgaat gcgggcattc tgctggttcc gtgctgtcgt
    17941 ggtggttccg cgttcaccac cggagccgat ggcacataca gtgacgctgg cggtgcctcg
    18001 gagaattcaa cccgctgggg tgtggacaag ccgctgtata aggaccttat cggtcgaaca
    18061 aaagcagcac tgaagaagaa cccgaaaaat gtgctgtttg ccgtggtgtg gatgcagggg
    18121 gaatttgatt ttggcggtac gccggtaaat cacgcagcac agtttggtgc gctggttgat
    18181 aaattccgtg cagacctggc agatatggca ggtcagtgcg ttggtggctc tgctggcggt
    18241 gttccctgga tatgcgggga cacgacgtat ttctggaagc agaagaacga atccacgtac
    18301 cagacggtgt acggcagcta taaaaacaaa acggaaaaga atatccattt cgtaccgttc
    18361 atgacggatg agaacggggt gaatgtgccg acgaacaaac cggaagaaga cccggacatt
    18421 ccgggtatcg gatattacgg ttcgaaatgg cgtgacagct ccgccacctg gacgtcacag
    18481 gacagggcga gccatttcag ttcatgggca cgccgcggga ttatttccga ccgtctggca
    18541 acggcgattc tgcgccatgc gggaagggtg gcgctaaacg tgggggcacc atcgacagta
    18601 tcagaggtgc gtccgtcatc gccttccggt gcagaagtca caggcgtcac aacactgctc
    18661 tcttaccttg ccagcgagtc agagggaagc ctgaaagtac agggatggtc agccagtggc
    18721 ggcagggcag aagtggtcag cgatgcggag ggaaccggag gtaaggctgt gaaggtgacc
    18781 aaggaagccg gtaaaagcag ctgggtactg gagtacgctg cgggcaacgg cgcggctctg
    18841 ttacagaaag ggggacagat tcgctgccgc tttaaggctt cgggagcgct ggcggcgaac
    18901 cagtatgtta tggcgtttta ctggccggta tcgtcactgc cacagggcgt tgtcctgacc
    18961 ggcgatgggg ggaataacct gctggcagcg ttctacatcc agacggatgc aaaagacctg
    19021 aatgtgatgt accacaatgc gaaagtggcg acaaacaacc tgaaactggg aagctttggc
    19081 gcatttgata acgaatggca tacgctggct ttccgctttg ccgggaataa cagccttcag
    19141 gtgacgccgg ttattgatgg tcaggatggc acaccgttca cgctgacgca gtcaccggtc
    19201 agtgcatttt cggcggataa gttgcatgtg acagacatta ccaaaagtgc gacttacccg
    19261 gtactgatag acagcattgc ggtggaagtg aataacgcgg acgccgctgc atgataaaaa
    19321 aagccgccag ttaccggaat ggactggcgg tggtaatacc tatggagaaa aaataaagga
    19381 acgatacttt cgtactctgt ttttttaaat gaaaacagtt cttattgtca acaataacgg
    19441 aaagaaatta tgacatttct gaaccagtta atgctgtact tctgtacggt ggtctgtgtg
    19501 ctgtatctcc tttcgggtgg ataccgggcc atgcatgact tctggcgcag acagattgac
    19561 aaaagggccg ctgagaaaat cagcgccagt cagtcagccg gaagcaaacc cgaagagccg
    19621 ctcgtttagc ggcaactttc ttaatcacac ctttcgacga gaaaatccca tgtcagaaat
    19681 tacatccctg gtcactgctg aagcagtgaa ggacgtcctg cgctctgaag aagtcctgag
    19741 cgcactgaaa cagaaacttc gccataacct ggaagcgcgt cttgatgcag aagtggatgc
    19801 cattctggat gaactgctgg gcgcaccggc agctccggag ccggaaggca tcgcgggaga
    19861 ggggagtgct tcagatagcg gtgaccccac acctgacagc gacatgatga tgtaagcatg
    19921 cgtcagggac catcggtgtg tgccggtggt cttttttatt gttgtgagct tccggattgc
    19981 gggaggcggg gtatgtacca gatggaaaaa atcacaacag gtgtgtcata caccacgtca
    20041 gcggtgggaa cgggctactg gttcctgcag ttgctggaca gggtttcccc gtctcagtgg
    20101 gcggcaatag gcgtgctggg gagtctgctg tttggtctgc tgacgtacct gacgaacctg
    20161 tatttcaaaa tcagagagga ccggcgtaag gcggcgcggg gagagtaagc tgatgagccg
    20221 gaaattccgc tatggtttat cagctgtcgt tctggcgctg attgccgcag gggcttctgc
    20281 gcctgaaata ctggatcagt ttctggatga aaaagaaggc aaccacacta cggcataccg
    20341 tgatggtgcg ggcatctgga ccatctgccg tggagccacc cgggtggatg gcaagcctgt
    20401 tattcctggc atgaagctgt cgaaggggaa atgcgaccag gttaacgcaa ttgagcgtga
    20461 taaggcgctg gcatgggtgg cgaaaaacat caaagtgcca ctgactgaac cccagaaagc
    20521 gggtattgcg tcattctgtc cttacaacat tggcccgggt aagtgtttcc cgtcgacgtt
    20581 ttataaacga attaatgcag gagatcgaaa aggtgcctgc gaagcgattc gctggtggat
    20641 taaggacggc ggcagggact gtcggaatcg ctcaaataac tgttacgggc aggtatcgcg
    20701 gcgtgaccag gagagcgcgc tggcgtgctg gagtatcgac agataagcag aatgttctgc
    20761 tgaaaaatgc cgttgaccaa cgcggacgga taacacgaaa tcctgcgaac tggcaaaatt
    20821 taagtgagta aaagtaaaaa ccccgtttgt tggcagcaag cggggttttg tgtttctgac
    20881 cttgaataag gcaagggaga aattatgggt agggaggttc tttccctgtg aggaagtata
    20941 aaagattctt tctgaggttg tccattatga aaggcattga ggtggagacg ccagcaagtc
    21001 tggatttaac aagagcggca gcttttgcca ttcgtattgt ggcaattgct gttctggtct
    21061 gggcaatccg ttggtggtga tatgaatcgt gtgctgtatg gcgtgcttat tgtcctgctg
    21121 cttggttgtg gatcgctgtg gctggcaaca gaccattacc gtgataacgc catcacctac
    21181 aaagagcagc gagatgatgc gaagacaaaa ctcagccagg cgaacgccac cattgctgac
    21241 atacagcagc gccagcgtga tgttgctgca ctcgatgcca gatacaccag ggagcttgct
    21301 gatgctaacg cgactattga aagtctccgt gctgatgttt ctgctgggcg taagcgcctg
    21361 caaatcgccg ccacctgtgc aaagtcaacg accggagcca gcggcatggg cgatggagaa
    21421 agctcaggac ttacagcaga tgctgaactc aattattacc gtctccgaag tggaatcgac
    21481 aagataaccg cgcaggttaa ctacctgcag gagtacatca ggatgcaatg cctcgattaa
    21541 cagagccagc tcaatcgctg gcctttttta tgaggagaga tataacctga atcaatgcca
    21601 gggcattatc aacaaatgga tcactgggta agctcgtttg gttttaaatg tccgtcgaga
    21661 atcagattgt gattttcccg atgcagaacc agtgtgccca tataatcagg tttaaatctc
    21721 catccattca ttttttttgc aattctggac agacagtgat tatgtgtgaa aatcacagta
    21781 acgtgatctg attttgatat tttaaggata ttatttactg cgttatcatc acatgtcgat
    21841 atgtccggaa tagttgctgt cttacctccc gaaaaaaaag tggctgtctg cattgttcgg
    21901 agtgtgtctg tagaatagag actgtagtat ggaaacattc ttctgaattt atctccatat
    21961 tgttgagctt tgttagctcc gtttactgtg attccctcat gtgctgaaag acatattctt
    22021 tgggagcgat cgcatctttc cccatgccgg atgagaaaaa taatgggatg tttctggtta
    22081 atctcagtga catcattcac atcaagtctg actggagttc ttgcaatgta tatgatggat
    22141 acagaaacga ttatgacaag taaaaatata agaagagatt ttgttagcgt ttgatttaac
    22201 atataaattc ccttgtctgt gttgaaaatg ggcattatta tgcgtaaatc ttatgtaatc
    22261 cttaaggatt gtctgacgtg gtgatatgac gcgaaataca gtgggtacac agaatgctgt
    22321 gaacaacgac gaaatgggca ctggtagcgg tactggtgct ttttttattg gttggttgct
    22381 caagtcttga taaagcgcgt tagctttttg gtattgcctc ttaggtctgt aaaattgccg
    22441 acggtgtccg gcagtgcatg cagggctgat cgtctatgag aacagaatat attgctaaaa
    22501 atgaagtgta cgtcaatgct tataaagcat gcaattctgt atttctaact attcaataaa
    22561 ataaattctt tctgtcgcag tgaatataca aatgttgatc agcgctcgat gtggcgacgg
    22621 gtttctatat caggagactt tagcatttat ccgcgaggca gcctatctgg tgctgtagtg
    22681 gaatgaagcg gatataacct aaataaggtt aaacattaat cagacatggc tctgctgtgt
    22741 gaaatctgaa aatttacagc agtcattgtg catcagtttt taacagagaa cgtcagaagg
    22801 tgacatggca aagctggact ggaaaaagct ggagcaggca tttcgacgcg aacatgtcaa
    22861 aaccggcata aagcttcagg actggtgccg tcagaacaat atcagttatg acacggctcg
    22921 gcggtacatt aaactgcgca aacactcccc cagaaataca gagaaaagtg cgcagaaaaa
    22981 tgcgcaaatt gatgatcaaa aaagtgcgca gaaatctgac ggcaactcca gtcatgacga
    23041 gccttcaggc gatgatggtt gtgatgaaaa atgcgcaaat ttggcagaaa cgaaacggac
    23101 tcgtggttcc agacttttgc caccttcaaa tgctttttct cagcgtaaca cccacgccgt
    23161 cagacaccgt ggatacgcga agtatctcga ggcagataac ctcatagatg atgcagtcgg
    23221 aatggaactt gccgatgaac tggtgtttac ccgtgcccgc gcactttcag taacaggaac
    23281 actgaaaaag atgttcgccg acctgaaaga ggcggctgac gtggaaacac gggtagctct
    23341 gtacgacaaa atacttaaag ccgaacaggc tcttgaccgg aatattgccc gtatcgagtc
    23401 aattgaacgc tcattgctga cgctggacgt cctggctga
//
LOCUS       DAZFFE010000076        23396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFFE010000076 DAZFFE010000000
VERSION     DAZFFE010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.076"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(17..136)
                     /locus_tag="ACHJ69_003180"
                     /pseudo
     CDS             complement(17..136)
                     /locus_tag="ACHJ69_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            214..1308
                     /gene="ddlA"
                     /locus_tag="ACHJ69_003181"
     CDS             214..1308
                     /gene="ddlA"
                     /locus_tag="ACHJ69_003181"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO5016112.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYTIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1332..1544)
                     /gene="yaiZ"
                     /locus_tag="ACHJ69_003182"
     CDS             complement(1332..1544)
                     /gene="yaiZ"
                     /locus_tag="ACHJ69_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO5016113.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1804..2112
                     /gene="yaiY"
                     /locus_tag="ACHJ69_003183"
     CDS             1804..2112
                     /gene="yaiY"
                     /locus_tag="ACHJ69_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO5016114.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYEHLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2171..3265)
                     /gene="yaiW"
                     /locus_tag="ACHJ69_003184"
     CDS             complement(2171..3265)
                     /gene="yaiW"
                     /locus_tag="ACHJ69_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO5016115.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3278..4498)
                     /gene="sbmA"
                     /locus_tag="ACHJ69_003185"
     CDS             complement(3278..4498)
                     /gene="sbmA"
                     /locus_tag="ACHJ69_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO5016116.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4850..6007
                     /gene="ampH"
                     /locus_tag="ACHJ69_003186"
     CDS             4850..6007
                     /gene="ampH"
                     /locus_tag="ACHJ69_003186"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO5016117.1"
                     /translation="MKRSLLFSAVLYAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVIRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYIAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6008..6631)
                     /gene="iprA"
                     /locus_tag="ACHJ69_003187"
     CDS             complement(6008..6631)
                     /gene="iprA"
                     /locus_tag="ACHJ69_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO5016118.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6717..9664)
                     /gene="ehaB"
                     /locus_tag="ACHJ69_003188"
                     /pseudo
     CDS             complement(6717..9664)
                     /gene="ehaB"
                     /locus_tag="ACHJ69_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
     gene            10190..11164
                     /gene="hemB"
                     /locus_tag="ACHJ69_003189"
     CDS             10190..11164
                     /gene="hemB"
                     /locus_tag="ACHJ69_003189"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO5016119.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(11204..12055)
                     /gene="tauD"
                     /locus_tag="ACHJ69_003190"
     CDS             complement(11204..12055)
                     /gene="tauD"
                     /locus_tag="ACHJ69_003190"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO5016120.1"
                     /translation="MSERLSITPLGPYIGAQITGADLTRPLSDNQFEQLYHAVLRHHV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIQTPPAGAILAAKELPSTGGDTLWASGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEAEHQRWREAVAKNPPLLHPVVRTHPVTGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     IPFYRAG"
     gene            complement(12052..12879)
                     /gene="tauC"
                     /locus_tag="ACHJ69_003191"
     CDS             complement(12052..12879)
                     /gene="tauC"
                     /locus_tag="ACHJ69_003191"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO5016121.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTLSIGTLAILFTVWWVVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(12876..13643)
                     /gene="tauB"
                     /locus_tag="ACHJ69_003192"
     CDS             complement(12876..13643)
                     /gene="tauB"
                     /locus_tag="ACHJ69_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO5016122.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKGIQGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGVEK
                     MQRLEIAHQMVKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQIQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(13656..14618)
                     /gene="tauA"
                     /locus_tag="ACHJ69_003193"
     CDS             complement(13656..14618)
                     /gene="tauA"
                     /locus_tag="ACHJ69_003193"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5016123.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVVYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVATSQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            15226..15897
                     /locus_tag="ACHJ69_003194"
     CDS             15226..15897
                     /locus_tag="ACHJ69_003194"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO5016124.1"
                     /translation="MDKVLDSAILSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIATVVMTTGNSGVDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     ENIIKNQIPSDVEIIRVYTMHDADRHQDHLAVYQASMVACRAIPQILGYETPSTWLSF
                     MPQVFESVKEEYFSLKLTALKKHKSQSQRDYMRPERLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            15907..17103
                     /gene="yaiP"
                     /locus_tag="ACHJ69_003195"
     CDS             15907..17103
                     /gene="yaiP"
                     /locus_tag="ACHJ69_003195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO5016125.1"
                     /translation="MKTWIFICMAVAILLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSHPSVTGT
                     EKLSEA"
     gene            17138..17662
                     /locus_tag="ACHJ69_003196"
     CDS             17138..17662
                     /locus_tag="ACHJ69_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO5016126.1"
                     /translation="MIIDESAGEVVIGANTRICHGAVIQGPVVIGTNCLIGNYAFIRP
                     GTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVANQAYLGAQVRTSNHRLDEQ
                     PVSVRTPDGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQLGPRVIVERNLPT
                     GTYSLRQELIRTGD"
     gene            17664..18437
                     /gene="yaiO"
                     /locus_tag="ACHJ69_003197"
     CDS             17664..18437
                     /gene="yaiO"
                     /locus_tag="ACHJ69_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO5016127.1"
                     /translation="MIKRTLLAAAIFSALPVYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGISLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSISLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            18626..18901
                     /gene="frmR"
                     /locus_tag="ACHJ69_003198"
     CDS             18626..18901
                     /gene="frmR"
                     /locus_tag="ACHJ69_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO5016128.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            18936..20045
                     /gene="frmA"
                     /locus_tag="ACHJ69_003199"
     CDS             18936..20045
                     /gene="frmA"
                     /locus_tag="ACHJ69_003199"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO5016129.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTPRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFELARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            20138..20971
                     /gene="fghA"
                     /locus_tag="ACHJ69_003200"
     CDS             20138..20971
                     /gene="fghA"
                     /locus_tag="ACHJ69_003200"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO5016130.1"
                     /translation="MELIEKHASFGGWQNVYRHYSPSLKCEMNVGVYLPPKAENEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPNLVMHHFPATARKSISGHSMGGLGALVLALRNPD
                     EYASVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(21198..21737)
                     /gene="yaiL"
                     /locus_tag="ACHJ69_003201"
     CDS             complement(21198..21737)
                     /gene="yaiL"
                     /locus_tag="ACHJ69_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO5016131.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKITQTQLINGRLAIARLLVDNNNEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(21839..23050)
                     /gene="mhpT"
                     /locus_tag="ACHJ69_003202"
     CDS             complement(21839..23050)
                     /gene="mhpT"
                     /locus_tag="ACHJ69_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO5016132.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILISSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(23226..>23396)
                     /locus_tag="ACHJ69_003203"
     CDS             complement(23226..>23396)
                     /locus_tag="ACHJ69_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="catalyzes the formation of pyruvate and
                     acetaldehyde from 4-hydroxy-2-ketovaleric acid; involved
                     in the degradation of phenylpropionate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO5016133.1"
                     /translation="LGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDM
                     IVDVALDLRNNK"
BASE COUNT         5905 a         6042 c         5661 g         5788 t
ORIGIN      
        1 actcaccaca tggtcattta agccaggaat aaattagctt cccgttcatc atttttgtga
       61 tcttgcacac atctttgaga taacgcactt tcttcaccag tacgttgctc gctttgtcag
      121 cagaagattg caacattctg tcagaaggcc gcgaatcccc cgttttgtcg tggtaatgta
      181 tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt aggaatcgtt
      241 tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa cattgtcgat
      301 gccattgata aaagtcgctt cgatgttgtg ctgctgggca ttgataaaca agggcaatgg
      361 cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca tattgcgttg
      421 cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca gcttatcgac
      481 gcacaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt ccacggtacg
      541 ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc gtttgtaggt
      601 tctgatgttc tggcttcagc ggcctgtatg gataaagatg tcaccaaacg tctgctgcgc
      661 gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg tcacaacatc
      721 agttttgccg aagtggagtc taaactgggg ttaccgctgt ttgtaaaacc ggctaatcag
      781 ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacacaat tgccgtcgat
      841 ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg tcgtgaaatc
      901 gaatgcgcag ttcttggcaa cgataacccg caagcaagca cctgtggcga gatcgtactc
      961 accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc gaaagtggta
     1021 gttccggcag ccattgcgcc agaaatcaac gataagatcc gggcgattgc cgttcaggct
     1081 tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac cccagagaac
     1141 gaagtggtga tcaacgagat caacaccctg cctggcttca ccaacatcag tatgtatccg
     1201 aagctgtggc aagccagcgg tctgggttac accgacctga tcacgcgttt gattgagctg
     1261 gcgctggagc gtcacgctgc ggataacgca ctgaaaacca caatgtaata ttcaaacgcc
     1321 cggtaatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat aatcaatccc
     1381 gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg ccaacctttt
     1441 gcttcaaatc ccagtaaccc caccacgcca ttaagaatga atataaggcc aatggcgaac
     1501 gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc tccgttaacg
     1561 caaaacaaca tccttgcata ttctgtaaca actgtctgtg gtgtagacaa cactttcgca
     1621 attgttaata aatttaactt aaggttgcta ttttgtgact cacaacgaag attcacgctt
     1681 cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta aatgacctgt
     1741 cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact tccggaggtt
     1801 tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg caatgcctct
     1861 tcaacgcctg gcaatattgc ctatgcgttg tttgtgctgt tttgcttttg ggctggggcg
     1921 caattgctga acctgctagt gcatgcgccc ggcgtctatg agcatttaat gcaggtccag
     1981 gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt tgggctgatc
     2041 ccgtttttag taggctgcct catttttgca gtagtggcgc tatggctgca ctggcgacat
     2101 cgccgccagt aagatttacg ttgccagatg cggcctaaaa cgccttatct ggcgtgacat
     2161 tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc ttcgcaaacc
     2221 aggccgtagt cagattgcgg gtgattttcg ggctttccag ttgaatgcca ggcaacattt
     2281 cgcggggtag tgatttaccg gttttcgctt cggcaagttg ataaactttc ttgtacagcg
     2341 ccgtctcctc aaaagagaag ctgtcccctt tctctaactg acggcgaatt tcgctgtcgc
     2401 tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg ggctctttac
     2461 tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc ttactgaccg
     2521 cgttctgaaa cgcggcatta cggctggcgt accagccagc gttaaaatca gcaaaacggt
     2581 atatcggcgc gctatagctg gcgggataat tcagtaaatg gtaagtacca aaccacaacc
     2641 cgccgcgacg actgaagact tcctgacgga ctgtaccgtc cattttccac ggatacccct
     2701 tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccacctgtg cgcaccggat
     2761 tgagcgaacc aaacagcgtc tgccccattg gcaccatgct gatcagatcg tcaaaaatcg
     2821 cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctatagctt ttgccgtttg
     2881 gcgatttgat ttttagcgcg gtatgcacca gaaaagcagg aatgtgcatc cgttcagcac
     2941 gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga tctgcctgat
     3001 aatttgactc ctgctgcgcc accgccagca ccgagcagac gttttccagc gtcggtgcca
     3061 gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg tctttcacgc
     3121 tcgcaggcat cttctgccgc acgacgctcg ccacatctat cgctttttcg ccctttttca
     3181 acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac aatgacagcg
     3241 aagatagagg attgacgcgc gacattactt ctccttttta gctcaaggta tgggttactt
     3301 cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg tagatagaca
     3361 tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg cgaacctgac
     3421 caaaaacgtt agtaatctgc gtcatcaggc cgagtgtaat cgtaccggca acaattgacg
     3481 gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg cgggcgatat
     3541 tgaaatacat atagtgaaaa tagaggcgga aatagttttt ccgtacggcg ctaaaaagct
     3601 cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc agctctttac
     3661 ggtaggcagc ctctacacgc tggtttttaa actccagccc cggcagtttg atccctacca
     3721 ctgccagtaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc agaccatacg
     3781 gaatgtgccc gacaatcggc agctccggca catgtgcgga gagcgttacc agcaccggca
     3841 ggaaggcgat caacgtcatg atagcgttga taaagctgac gcccatattc tccagcgttg
     3901 aagcaaaacg catggtgtct tcctgcacac gctgcgcggc cccttcgata tgacgcagtt
     3961 gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac acgtagtgac
     4021 tgacaaagaa gttgttcagc acactgatca ccaccgcgat cagcgcaatc cccagaaaga
     4081 cgcccacttc gcggtaaaat tgttcgatgg tgactttatg cggcgaactt agcgccgttt
     4141 gaatcagatc atagaacggc gcataccagg cgttgacggc aaccccgact tccaccaaaa
     4201 accaggtgac gaagataatc agcgcagtac cgagtattga ccagtattgc caacggtgcg
     4261 ggctatagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag taagcgtaaa
     4321 aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc ccggaagcgc
     4381 cggtgatacg cgccacccaa tcacccccac cggcttgcca gaagataacg gcaatcaatg
     4441 cccaaacaaa ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac ttaaacatat
     4501 gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc atgaccgcta
     4561 caaagcgtca gccaaatgaa actgcccatt gtgacccata gcaaggcgct tagttcgcca
     4621 gcctgaccat aagaattgtt tcgacgggtt tcacaaacca aagattgttt acataagcgg
     4681 attaacacgc gcaggatcac aaatgcaggc attggtgagc aggaaaaaga tcgtgtttac
     4741 gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat ccatcaatga
     4801 gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt tgaaacgtag
     4861 tctgcttttt tctgccgtgc tgtatgcggc gtcattgacc tccgtccatg cggctcagcc
     4921 gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc atatttttta
     4981 cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc gcgtctttcg
     5041 cagttatggc gaaacgcgac ctggtaataa cgttcgcccg cagctggatt ccgtcatacg
     5101 tattgcttcc ctcaccaagt taatgaccag tgagatgctg gtgaaattgc tcgaccaggg
     5161 gaccgtgaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac gcgtaccgac
     5221 ctacaatggg acgcccatta cgctggtgaa tctggcaacc cataccagcg ccctgccccg
     5281 tgaacagccc ggtggcgcgg cacatcgtcc ggtctttgtc tggccaacgc gcgagcaacg
     5341 ctggaaatac ctttccactg ccaaactaaa agccgcgccg ggcagccagg ccgcgtactc
     5401 caacctcgca ttcgatctgc tggcagatgc actggcgaat gcgtccggca agccttatac
     5461 ccagttgttt gaagagcaga ttacccgtcc gttggggatg aaagacacca cctacacccc
     5521 ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc cgtgcaataa
     5581 cacgctggcg gcaattggca gcggcggcgt ctattccacg ccaggcgata tgatgcgctg
     5641 gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggctg atcgcatgca
     5701 gacgttgatt tatcagcgcg cgcagtttac aaaagtgatt ggcatggatg tccccggcaa
     5761 agcggatgcg cttggtttag gctgggtata tatggccccg aaagagggtc gtccggggat
     5821 tattcagaag acaggtggtg gtggcggttt cattacctat atagcgatga tcccgcagaa
     5881 gaatattggt gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta aaaatatgag
     5941 cgatggcatc aatgacctgg tgaccgagct aagcgggaat aaaccgttgg ttatccccgc
     6001 atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct ttattcattt
     6061 cgatatagcc gcctttacgc aaagcagcga gaacttccgc aacgacagaa cgcgatatac
     6121 gtgtacgttg atggatataa ttcatcacgc caatacgtga tcgcaattct tcattccagt
     6181 caatcattga taataacgtc gcgcggattt gttcgtagga attatgccca atgagctgaa
     6241 cgtcgcgtaa ttccagaatt ctattttgcc aggctaacca gtaaaaagcg tctcgccaga
     6301 gttgattttg ttcaataaga gtaatggttt gtttggctgg caaatgatat cccgtacagt
     6361 ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca gccagcccca
     6421 taatataagg agcctgtgta ataccgataa gtacgttttc ttctctacgc aaagagataa
     6481 cgccctctaa aataacgaaa gtatcttcgc tattgacatc actggaaaat ataacttgct
     6541 tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg agcttaccaa
     6601 attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta atgcccgtca
     6661 aatccttgac gggcattatt tagattaaat tacctatttc ttcggagtga agaatattac
     6721 caggtatatt taacacccac attcgcggac cagtcttgat ctacgtcacc accaccgagg
     6781 tagttagcat cggtataggc gctgaagttc ttggtgaagc tgaactgagt acccagacca
     6841 acacgtaccg cagacccttc agtaccgtta tcgatggaat caccgttcac atcgttatcg
     6901 ttgttagagt cgtcgtagac gtaagccagt ttgaagtacg gagtcagagc ctggtcttcg
     6961 ctgtaggtga aggtataacc tgcatctaca cccagttcat aacgcatgct gtcgtaagac
     7021 tgaccatcaa ctttcatgtc gttgctcagc tggtaatcat caccagactg gaacagacca
     7081 gaaacgctgc cgtaaggagt cacgtaacct gcatcaccca gtttgaagtc gtaaccggct
     7141 ttcaaaccaa agccccaagc gtcggagttg gtgctgccgt caacgtaagt accgttgctc
     7201 atggttgcag acaggtcgtt gttgaagtga gagtagctca agctaccgtc aacaaagacg
     7261 ttgttcgcga agtgagcaga agagtagatg taggcagtct ggctgtcttg atccacctga
     7321 ccagaacggt cattcatatc acctttagcg aagcctgcag ccgcaccgac gatccactta
     7381 gcgttgttac cgtcaatttt ggtatcaaca ccgaccatga tgccgttaac atcctgatca
     7441 tagttgatgg tgccgttgtc gccgttgaag ttaccaccga agtagcttac ccatgcgccg
     7501 ccgttatcag ccagaccatg acgagagttg gtcagacgag tacaacggtg tcttgttcca
     7561 ggttccagat attggtgttc gcagacggga tgctcagcgc catgttagcg tagtcggtca
     7621 gctccatctg ttgcagaaca acggtgttac cacgctgctc agcctgatag gtgtatgcac
     7681 ccaggtcagc tttgttagca gcagagaagg tcgcgttgct gtttttgtcg ttgacgtaga
     7741 tcagctcgtt gccgttgtag tcagcgatag aaccagcacc agtagcgttg tcgatacgaa
     7801 ctttgtagtt accggttgca gctgcaacat ggtcaccgtt gttatcaact tcgttctgac
     7861 cagcttcgat agaagcagtg ttgtcgttat cgccgttacc gttaatggtc aggtgaccat
     7921 cagagttcat tgcgattaag ccataaccgt agtttgactt ggtagtatcg ttggtacggt
     7981 cgttggtcag gtctgcatca agtacgaagc ggttgctttc gatgttgaac acacctgcgt
     8041 gaatgttacc actggtatca gtagtgctgg tcaggttcag agtatcaacc tgcagagcat
     8101 tcgccgcagt gttacctaca tccaggatac cgtcgttagt cactttgatg ttgttagcat
     8161 acagagcagc atcacctaca gcccagccag cagaaacttc atcagaaatg gttacggtac
     8221 tgttgtcgac agtcagtgaa tcagttgcaa catgaccatc ttcgttgatg ttcagcgcag
     8281 aaagaccagt cagagagata gagtcagaaa tcagcgttga atcagcaaca ttaacagttg
     8341 aaccactgtt gatgctcaga gtatcaatca gagaagtttt ggtggtatcc cactgtgaac
     8401 cggtgttcag ggtcacattg aacagaccgc tctggtaaac ttcgttaccg atgatgtgat
     8461 caaattcgct gtattcagag tcgctgtttt ctacaccaac agttgatgaa ggccacaggc
     8521 tgttagctgc gatgtcgatc agagttgcag tggcttcagt attgattcca tatgtatagg
     8581 agtcatttac accatcgtca tcagtatcaa caccaatctg atgcgcagac attgccgcac
     8641 cgacccactt gctgccattg ttcagggtca gatccaggcg atcagtacca tcccaaccgt
     8701 tagtatctac atcaccgtca gcatcacgat agctatcacg accttgtggg aagaagttct
     8761 catcaaaatt acttgagaag aatacatcgc ccatcagtgt ggaattgtcg aatgttgcag
     8821 tggtttgcat ggcgttatca gcatatggat gtgcaataac agctaatgca acatcatcag
     8881 cattccaccc atcaccattg taatcactag cattaccagt attaccaaac caaccagtag
     8941 taccttcatc agtccatgaa ccagaggtca cggtagaatc tttaacagtg atggtgttat
     9001 taaatacttc gctgctgttt acaccagtgc tgacagtgtt gtcgtcgata tcttcccact
     9061 cataaccctg agtcagcgta atacctgcta catgagagtt attagagatg gacagatcaa
     9121 cttcctgatc cagagtgatg gcagtaccaa tgttaaatac atttacaaca tgagtgtcag
     9181 cggcattatt gtaggtgccg ttatatgtgt agtgttcgta gttgtcatcg atcgttgaat
     9241 tatcaacggt cagcgccaga cgatcatggt agtagtcatc cgcacgatca gcacaatcag
     9301 ttgtcatgca ctcggaataa atcatgccgt gaatagtgct gtttttgatt gtcaggctat
     9361 tggcattggt attagtagaa ataccatcgt ccagatagta ggtagaaatc acgccgttaa
     9421 cggtagcgtt attaatgacc ggatagatat cgccgttgta ataactatca gcggcatagt
     9481 tattccagcc tacataacca tcatagtaaa cgtcatcagc ataggtggcg tcgttgtagt
     9541 gatggaaagt attgtaagtt tgaccagaaa tatcgttggt cgctgcatta gcctgagagg
     9601 tgatagccag agtgcaagcc aatgctaatt gtgatactac aagtttcttt ttccaggagt
     9661 gcatttgtca tccctcctca gggacgtaat aagttgatcc atcaattatc aatgcataga
     9721 aaacatatta tgtttcgcgt ttacgattat gcggcgttat agaggaatgg ttcaattatt
     9781 ctttccccta agtccgattg cggacatata taaagcacgt gaacacacac aaagaaagaa
     9841 ataacaaaaa cacaatattt caatacgtta atgaaagcgt taattttaaa acatagacca
     9901 tgaaaatata aaacaacaaa aacagacaaa taatgtatca ataagcaatg aactttaagc
     9961 tattcacttt ttggaaacgt caccaaatcc ccaaaaacaa taatctatgg ctataaataa
    10021 aaattaaaaa tattttcagt gaatatgaaa ttattgctat ttctattaca aagatgtcct
    10081 gacctctttt tcattctctt tttgtcaaca tcgcgacaac tttcgtaaaa cacacccagc
    10141 ctgcttcagg tatactatgc tcctcgattc cacaaacatc aggcagacca tgacagattt
    10201 aatccaacgc cctcgtcgcc tgcgcaaatc tcctgcgctg cgcgctatgt ttgaagagac
    10261 aacacttagc cttaacgacc tggtgttgcc gatctttgtt gaagaagaaa ttgacgacta
    10321 caaagccgtt gaagccatgc caggcgtgat gcgcattcca gagaaacatc tggcacgcga
    10381 aattgaacgc atcgccaacg ccggtattcg ttccgtgatg actttcggca tctctcacca
    10441 taccgatgaa accggcagcg atgcctggcg ggaagatgga ctggtggcgc gtatgtcgcg
    10501 catctgcaag cagaccgtgc cagaaatgat cgttatgtca gacacctgct tctgtgaata
    10561 cacatctcac ggtcactgcg gtgttctgtg cgagcatggc gtcgacaacg acgcgactct
    10621 ggaaaattta ggcaagcaag ctgtggttgc agctgctgca ggcgcagact tcatcgcccc
    10681 ttctgccgcg atggatggtc aggtacaggc gattcgccag gcgctggacg ctgcgggctt
    10741 taaagatacg gcgattatgt cgtattcgac caagttcgcc tcctcctttt atggcccgtt
    10801 ccgtgaagct gccggaagcg cattaaaagg cgaccgcaaa agctatcaga tgaacccaat
    10861 gaaccgtcgt gaggcgattc gtgagtcgct gctggatgaa gcccagggcg cagactgcct
    10921 gatggttaaa cctgccggag catacctcga catcgtgcgt gagctgcgtg aacgtactga
    10981 attgccgatt ggcgcgtatc aggtgagcgg tgagtacgcg atgattaagt tcgccgcgct
    11041 ggcgggtgct atagatgaag agaaagtcgt gctcgaaagc ttaggttcga ttaagcgtgc
    11101 gggtgcggat ctgattttca gctactttgc gctggatttg gctgagaaga agattctgcg
    11161 ttaatgatca ttatgccggg ttatccaacc cggcatttcc cccttacccc gcccgataaa
    11221 acggaatatc cccaagaatc gtcgcccgat gcataatccg ccgctgtggc aggtaatccg
    11281 cattggcata gtgctgagtc acgcggttat cccaaatcgc aatatcattc ggttgccagc
    11341 gccagcgcac ctgaaactcc ggtttagtga tatgggcaaa caaaaaacct aacaaggctt
    11401 cgctctcttt ctcgctgaca tcaacaattc gtgtggtaaa accttcgttc acaaacagcg
    11461 cctgtttacc ggtcaccgga tgcgttcgca ccaccggatg tagcaacggt ggatttttcg
    11521 cgaccgcctc acgccagcgt tgatgctcag cctcggtttt gcggtacttg tattccggga
    11581 acgatttacg gaaatcatgc tccgcacgca acccgctcaa cagctggcgg aagggaacag
    11641 agagcgcctc atatgcggca ataccactgg cccagagcgt atcgccgccg gtcgagggta
    11701 actctttcgc cgccagaatc gcccctgcgg gtggcgtttg aataaatgtc acatcggtgt
    11761 gccagttgtc gttatccggc ggattatcgt tatgggtatc caggacgatg atctcgtcaa
    11821 ccccttcagc atgagggtaa acagggtgaa tatgcagctc gccaaaacgc tgggccagcg
    11881 cgcgttgctg ctgcggcgta atagcttgat cgcgcagaaa caccacatga tggcgcagca
    11941 ccgcatggta aagctgttca aactgattat cgcttaacgg gcgcgttaag tcggcccccg
    12001 taatttgtgc gccaatatac ggccccagcg gggtaatgct cagacgttca ctcattgtac
    12061 ttctccatgc cagggcgtca ggcggcgctg taacgcgcgc agacccagtt ctaaaagaaa
    12121 ggcgataatc gcaatcaccg cgatccccgc cagcaccacg tcagtcgcga gaaattcacc
    12181 cgctgactga accataaatc ctaaaccgcg cgttgcggca atcagctccg ccgccaccag
    12241 cgtagaccag cccacaccca gaccaatgcg taatccggtg aggatttccg gcagcgcgcc
    12301 gggcaaaatg acaaaccaca gcacctgcgc acggctggca cccagcgact gggcggcacg
    12361 aatgcgaacc tgctgcgcgc ttttcacccc cgccagcgcc gacatcgcca ccggcgcaaa
    12421 aatcgctaga tagatcagta agatcttcga ggtttcgcca ataccaaacc agatcaccat
    12481 cagcggcaaa taagccagcg gcggcaccgg acgataaagc tcgattatcg gatccagaat
    12541 gccgcgcacc gtcgggctta gccccatggc gatcccgacc ggaataccga tcaacaccgc
    12601 cgcgagtagc gccagcacaa tgcgcgtcag gctggctgcc agatgctgcc acagcgtggc
    12661 gtccataaat ccctgcggcc cggcaatggt gagcagtttt gccagtacct gttgcggtgg
    12721 cggcaaaaac agcgggctaa tcagttgcag cgccgcgact acccaccata cagtgaataa
    12781 aatcgctaac gtgccaatgc ttaaggtcac ctgacgcgag agcggccagc gccatttaag
    12841 ccgatgcgaa tgcagttttt cattaatgag cacactcatg agaacgcctc ccgttgctca
    12901 aatacgcggc ttaaaacata ttcgcgcatg gcgatgaatt gtggatcgga cttgatgctg
    12961 cggctcgact ctcccgcaac aaagcggcgg gcgaagttga gcggcagccg ctccagcacg
    13021 cggcctgggc cgggtgaaag cagaaccagt tcagtcgcca taaacaccgc ttcttctata
    13081 tcgtgggtaa tcaacagcac ctgctttccc gtctcctgcc agagtttcag cagcagggtt
    13141 tgtatctggt cgcgggtgaa ggcgtccagc gcaccaaacg gttcgtcgag taataacagc
    13201 tggggatttg ccgccaacgc gcgggcaatc cccacccgct gacgttgacc accggaaagc
    13261 tgccagatgt agcgtttttc tgcgccttcc agcccgactt ttttcaccat ctggtgtgcg
    13321 atttccagtc gctgcatttt ctctacacct gccagttgca ggccgaacgc cacgttgtcc
    13381 tgtacattgc gccacggtaa caacccttca ttctgaaaaa ctacgccacg ctctgctccc
    13441 ggtccctgaa tacccttacc cgccagttga atgctgccat gctgataagg cacaaaacca
    13501 gcaatcaaat tcagcaacgt agttttgccg cagccagacg gccccagaac taccagaagc
    13561 tcgccgcttt ccagcgtcag gttgatatct tccagtgccg gtttgccgcc ataatcggcg
    13621 taaagatgag aaatttgcag catctgcgcc tccttttatt gcacgaagcg cgaggtaacg
    13681 tactggctgt aatcattcgc tacagccggg actttgccct gctctttcaa aaactgcgcg
    13741 gtgtcgatga tcgctttgtt caccggtccg gtcagttctg ccgtttgttg ctgcggcgtc
    13801 agataggtat ttcccttcac cagccccgga acgtcacctt ccggcacgcc gcttaaccgc
    13861 gccagtttgc tgatgttttc cggctgtttc agccacgcgt ctgggttagc aatgtacggt
    13921 tgctgagcat cgatggcgct tttagcgaac gctttcacga cctcaggatg tttctcggca
    13981 aaatctttcc gcacgaccca gacgtccagt gtcggcgcgc cccactgccc gacctgttca
    14041 gaatcggtca gcaccttgcc atctttttcc agggcgttaa ccgccggtgc ccagacataa
    14101 gcaccatcaa tatctccccg ctgccatgcg gcgataattg cgggcggctg caggttcaca
    14161 atctcaactt gcccaggttt aataccccag tgtttcagcg ccgccagcag gctgtagtgg
    14221 gtggtggaga taaacggtac ggcgatgcgt ttaccaatca agtcttccgg tttactgata
    14281 gttttcttta ccaccagcgc ttcggagtta cccagttttg acgccagcaa gaagacttca
    14341 atcggcacct gttggctggt tgcaaccgct aacgggctgg aaccgaggtt gccgatttgc
    14401 acatcgcccg aagccagcgc ccgcacgatg ctggctccac tgtcaaactt acgccagtcc
    14461 acggttgctc cgctttcttt agcaaaggtg ttgtcggcct gagccacttt cgccggttcg
    14521 gcggaggttt gatacaccac ggtgacgttc accgcctgcg cctgaaaagc gatgaatgcc
    14581 agtgcggcaa gaagtgtgtt acgcgatgaa attgccatga ttgtctgctc ccccgtcttg
    14641 ttatgggagc agtattcagg aataaaaaca ttcattaaaa gaattagtcg ttatcgtaca
    14701 gatgatttta ttcttagcaa aaaagcggca aaggaaagat taaatatgca attacgccca
    14761 ctctgataac tcagtaaatt actgcacttc attttattaa tgcataaaca agcaggttct
    14821 aatgtctgtt attttattaa cagcaacaca tataatgaaa ccattatatt cacattgcat
    14881 atacaaatgt atcattctca atcaactaca taatgtattc acataatgca tataatcaat
    14941 atttgtgcat agaacatatt tttaatatgc aattaattga caaactaaat cacatttaaa
    15001 tacaatatat aaaacaaaat aaccatacaa accaaacaca caaacactta acatatgaac
    15061 tacataaaag attacacact cttatccccg tacctgacag ccccttgttt ttcagctatc
    15121 agctattttt aacacttaaa aattagcatc tgtctgcgtc agaggttacg tctctggggg
    15181 cgtcgttgtg cttgtagtat aataaataaa atggagtgtc ataaagtgga taaggtttta
    15241 gattcagcga tcctttcttc ggcaaataaa agaaagggta tccttgccat tggcgcgcat
    15301 cctgacgata tagaattagg ctgtggcgca tcgctagctc gccttgcgca aaaaggaatt
    15361 tatattgcta ccgtggtgat gactaccggt aattctggcg tagatggaat aatcgatcgc
    15421 cacgaagaat cacgcaacgc tctaaagata ttagggtgcc accaaactat tcatcttaat
    15481 tttgctgaca cccgcgctca tttacagctc aatgatatga tttctgccct ggaaaacatc
    15541 ataaaaaatc aaattccctc tgatgttgaa attatacgtg tatataccat gcatgatgct
    15601 gaccgccatc aggatcatct tgcagtctat caagcctcaa tggttgcctg tcgcgctatt
    15661 ccgcaaattc tcggctatga aacaccaagt acctggcttt catttatgcc tcaggttttt
    15721 gaatccgtga aagaagaata tttttctctt aaactgactg cgttaaaaaa acataaaagt
    15781 cagagccagc gagattatat gcgcccggag cgtctgcgtg cagttgctca atttcgcggg
    15841 caacaggtca atagcgatct gggtgaaggt tttgttattc ataaaatgat tctttgagca
    15901 aattgcatga aaacctggat ctttatatgt atggccgtag caatattgct atggtttctg
    15961 agtacgttaa ggcgtaagcc cagccaaaag aaaggctgta ttgacgccat tatacctgct
    16021 tataacgaag gcccgtgtct ggcgcagtca ctggataatc tgctgcgtaa cccttatttt
    16081 tgccgggtaa tttgcgttaa cgacggctcc acggacaata ccgaagcggt catggcggaa
    16141 gtcaaacgca aatggggcga ccgctttgtt gccgtcacgc aaaaaaatac cggtaaaggt
    16201 ggtgcgctga tgaatggcct caattacgcc acctgcgatc aggttttttt aagtgatgcc
    16261 gacacctatg ttccgcccga tcaagacgga atgggctata tgctggcgga aattgagcgt
    16321 ggcgctgatg ccgtaggcgg cattccctct actgcgttga aaggcgcggg tctgttacca
    16381 cacatccgcg cgaccgtaaa gttgccgatg attgttatga agcgcacgct acagcagctt
    16441 ctgggcggcg caccgtttat catcagcggt gcctgcggga tgttccgtac tgatgtattg
    16501 cgtaagttcg gtttctcgga tcgtactaaa gtcgaagacc ttgatctcac ctggacattg
    16561 gtggcaaacg gctaccgtat tcggcaggcg aatcgctgca tcgtataccc acaggaatgt
    16621 aacagtccgc gtgaggagtg gcgccgctgg cggcgttgga ttgtgggcta cgcggtctgt
    16681 atgcgcctgc ataaaagact tttatttagc cgcttcggta tcttcagtat atttcctatg
    16741 ctgttggttg tgctttatgg cgttggtatt tatctcacta cctggtttaa tgaattcatc
    16801 accaccgggc cacatggtgt ggtgttggca atgttcccgc ttatctgggt cggcgtagtt
    16861 tgtgttattg gtgcatttag cgcctggttt catcgttgct ggttgttggt gcctttagca
    16921 ccgctttccg ttgtgtatgt attattagct tatgccatct ggattattta tggacttatt
    16981 gcctttttta ctggacgcga acctcagcgc gacaaaccca cccgctattc cgcactggtg
    17041 gaagcgtcaa ccgcttattc ccacccttct gtcacaggaa ctgaaaaact atctgaagct
    17101 taatgttaaa gagaaaaata ttcagattgc agaccaggtg attattgatg aaagtgccgg
    17161 tgaggttgtt atcggcgcga atacccgtat ttgccacggt gccgttattc agggtccggt
    17221 agtgattggc acaaactgcc tgataggtaa ttatgcgttt attcgtcctg gcacaataat
    17281 cagcaatggc gtaaaaattg gttttgccac cgaaattaaa aatgcggtta ttgaagcgga
    17341 agcaacgatt ggtccgcaat gctttattgc cgactcggta gttgcaaacc aggcatattt
    17401 gggcgcacaa gtacgaacca gtaatcatcg tctggatgaa cagcccgtgt ctgttcgaac
    17461 tccagatgga attatcgcta ccggatgcga taaattaggt tgttatatcg ggcagcgttc
    17521 acgccttggc gtacaagtta ttattttgcc tgggcgaatt atttccccga acacgcagct
    17581 tggcccgcgc gtgattgtag aacgtaattt acctaccgga acttattcac tccgacaaga
    17641 acttatccgt acaggagatt aaaatgataa aacggacgtt attagcggcg gcaattttta
    17701 gcgcattacc cgtttatgcc gggttaactt ccattaccgc gggctacgat tttaccgatt
    17761 attctggcga tcatggcaac cgtaatttag cgtatgctga actggtggcg aaagttgaaa
    17821 acgcaacgct gctttttaat ctttcccagg ggcgtcgtga ttatgaaact gaacatttca
    17881 atgccactcg cggccagggt gcagtctggt acaaatggaa taactggctg acaacccgaa
    17941 cgggtattgc ctttgcggat aatacgccgg tctttgcccg ccaggatttt cgtcaggata
    18001 ttaacctggc cctgttgcca aaaacgcttt tcacgaccgg ttatcgctac actaaatatt
    18061 acgatgatgt cgaagtcgat gcctggcaag gtggcatatc actctatact ggcccggtga
    18121 tcaccagcta tcgctatacc cattatgact ccagcgatgc aggtggtagt tacagcaata
    18181 tgatttccgt gcgtctgaat gacccgcgcg gcactggtta cacgcaacta tggctaagcc
    18241 gcggaacagg tgcttacacc tacgactgga cgccagaaac acgctacggc agcatgaaga
    18301 gcatcagttt gcaacgtatt caaccgctga ctgagcaact taatctcggg ttgacggcag
    18361 gtaaagtgtg gtacgacacc ccaaccgatg attataacgg tctgcaactt gcagcccatc
    18421 tgacctggaa attctgattc cttctgccgc ccgctatccg gggcggcctt ccctgccgat
    18481 tagcccctat cccctttcct ctttgttttc cgactacatt caccggataa attttattct
    18541 ccagtgttat atactatagg ggggtatgca ttgacatata gcataccccc ctatagtata
    18601 ttgcgtgcag atgatgaggt gcgaaatgcc cagtactccg gaagagaaga aaaaggtcct
    18661 tactcgagtt cgtcgtattc gggggcagat tgatgctctg gaacggtcgc tggagggtga
    18721 tgccgaatgc cgtgccatac tccaacagat cgctgccgtt cggggcgcgg ctaacggact
    18781 gatggcagaa gtgcttgaaa gccatatccg ggaaacgttt gaccgaaatg actgctacag
    18841 ccgcgaagtc agccaatccg ttgacgacac tattgaactg gttcgtgcct atcttaaata
    18901 gctgaatcta ttaccatatt gaggaagagc gagagatgaa atcacgtgct gccgttgcat
    18961 ttgctcccgg taaaccgctg gaaatcgttg aaattgacgt tgcaccaccg aaaaaaggtg
    19021 aagtgctgat taaagtcacc cataccggtg tttgccatac cgacgcattt accctctccg
    19081 gcgatgaccc ggaaggtgtc ttcccggtgg ttctcggtca cgaaggggcc ggggttgttg
    19141 ttgaagtcgg tgaaggcgta accagcgtca aacctggcga ccatgtgatc ccgctttaca
    19201 ccgcagagtg cggcgagtgt gagttctgtc gttctggcaa aaccaacctg tgtgtcgcgg
    19261 ttcgcgaaac gcagggcaaa ggcttgatgc cagacggcac cccccgtttt tcttacaacg
    19321 ggcagccgct ttatcactac atgggatgct caacattcag tgaatacacc gtggtagcgg
    19381 aagtgtctct ggccaaaatt aatccagaag caaaccatga acacgtctgc ctgctgggct
    19441 gtggcgtgac caccggtatt ggcgcggtac acaacacagc taaagtccag ccaggtgatt
    19501 ctgttgccgt gtttggtctt ggcgcgattg gtctggcagt ggttcagggc gcgcgtcagg
    19561 cgaaagcggg acggattatc gctatcgata ccaacccgaa gaaattcgaa ctggcgcgtc
    19621 gcttcggtgc caccgactgc attaacccga atgactacga caaaccaatt aaagatgttc
    19681 tgctggatat caacaaatgg ggtatcgacc atacctttga atgcatcggt aatgtcaacg
    19741 tgatgcgtgc agcgctggaa agcgcgcacc gcggctgggg tcagtcggtg atcatcgggg
    19801 tagcaggctc cggtcaggaa atctccaccc gtccattcca gttggtcacc ggtcgcgtat
    19861 ggaaaggttc cgcgtttggc ggcgtgaaag gtcgttccca gttacctggt atggttgaag
    19921 atgcgatgaa aggtgatatc gatctggaac cgtttgtcac gcataccatg agcctggatg
    19981 aaattaatga cgccttcgac ctgatgcatg aaggtaaatc cattcgaacc gtaattcgtt
    20041 actgatttcc cgctggttta ccccgtccag ttcagacggg gttcttaata ctctccccgg
    20101 gcagccgtcc gggggattaa ccgtgagata atgactgatg gaactcattg aaaaacatgc
    20161 cagctttggc ggctggcaaa atgtgtatcg gcattattcc ccatcactga aatgtgaaat
    20221 gaatgtcggc gtctatctcc caccaaaagc cgagaatgaa aaattgccgg tgttgtactg
    20281 gctttcaggc ctgacctgca acgagcagaa tttcattact aaatcgggga tgcagcgtta
    20341 cgcggctgag cacaacatta ttgttgttgc gccggacacc agtccgcgag gcagtcatgt
    20401 cgcagatgct gaccgttacg atctcgggca aggtgccggg ttttacctga acgcgacgca
    20461 agcgccgtgg aatgaacatt acaaaatgta tgactatatc cgcaacgagc tgccgaattt
    20521 agtgatgcat cattttccag caacggccag aaagtctatc tcaggtcatt ctatgggcgg
    20581 gctgggcgcg ctggtgctgg cgttacgtaa cccagatgaa tatgccagcg tctcggcgtt
    20641 ttcgcccatt gtctccccat cgcaagtacc gtggggacag caagcctttg ccgcatatct
    20701 tggtgaaaat aaagatgcct ggttggatta cgacccggtg agtcttattt cacaaggtca
    20761 acgcgttgcg gaaatcatgg ttgatcaggg attgagtgat gatttttacg cagaacagct
    20821 gcggactcca aatcttgaaa agatctgcca ggagatgaat atcaagacgt taatccgtta
    20881 tcacgagggt tatgatcaca gctattattt tgtctccagt tttattggcg agcatattgc
    20941 gtaccacgcc aataaactga atatgcgttg ataatagtgc acgactgccg gatgcggcgt
    21001 aaacgcctta tccggcccac atttcgctcg taaaccgtag gcctgataag acgcgaaaag
    21061 cgtcgcatca ggcatctgcg cacggctgcc ggatgcggcg tgaacgcctt atccggccta
    21121 cacttcgccc ataaaccgta ggcctgataa gactcgcaaa gcgtcgcatc aggcatctgc
    21181 acacgattta tctatgttta ccacatcaaa tcatcaggca ctttgaagtc ggcatacgga
    21241 tcgtcttcat cctgctcttc ggcgctgagc gcgctgtgca acacaatact gctggcatca
    21301 cgctgagcaa ttttatcggc aacgctggcg ggaataatgg cgtattcacc ttcgttattg
    21361 ttatcgacca acaagcgggc aatcgccaga cgaccattaa tcaactgcgt ttgcgtgatt
    21421 ttatcgacaa agattttctt aatcagattg ccgtcagtga agttaaaacc aatatcgcca
    21481 ttggcaatgg taattcggtt catttcaatg agctgcttca cttgggcttt atattctttc
    21541 gccaacgccg cttgtttttg ttgctcgcta agctgtttat cacgctcaag ctgtgccttt
    21601 ttattttctt ctaccgccgc acgagcttca cgcgcctgaa cacgcgattt tttcgccgtt
    21661 ctctccacct tcgccgcttt tttgctggtg actagtcccg ctttgagcaa ctgctcttgt
    21721 aaggtaagtt ttgccatctt tgtttccaaa cccgctgaat aatttttagc gagtatacct
    21781 gtaaacgccg taggtgtatt gggggcaagt ccctttcgcc cctacttaat aaggcgcgtc
    21841 aggcatcggc gcacggctgc attcgtgatc tccggctcat caaaataaac actgccaggc
    21901 cggcaacaag aatacccggc gcagaagcgg ccattacgcc aaccgtgcca gtgcctaatg
    21961 ccagcatttt cccggccagt aacggaccgc tcatagcccc cagacgccct accgccacgg
    22021 ctgttcccac acctgttgcg cggatctgcg aactgtaaaa caacggtgcc agggcataca
    22081 aaacgctttg cccacctgtc gcaaacaacc ccgcgacgaa tcccgccagc aacataccgt
    22141 taaatgacga caccgttccc agcgccagca gcgaagctaa catgccgcta taaatcagta
    22201 gcgacatggt tactggacgc agcttatcca tcaatgcgcc caacattaac gtcccgcttg
    22261 ccgcacccat ttgtagggca aacatcaccc ctgccgcctg cgatggctgg aatccttgct
    22321 ccaccaaaag cagcggtagc cagttgatca acatgtagac caccagcaga gtgaagaaat
    22381 aacacaacca cagcagcagc gtcgcggtag ccgtttctgg cgcaaataac gcacgcagtg
    22441 gtggcgcagc ctgtttttcg ccagcaaaaa ccgccgactc cggcagccag cgcattaata
    22501 gcggcaccag aatcaacggc accacaccac ctacccaaaa caccgtttgc catgctaagt
    22561 ttgcccccgc gaaacccagc gtcgccgcca gcgccgcgcc aatgggaaca ccgcaataca
    22621 tcaggctcac tgccgtccca cgaaaacgtg gccccgcggc ttcagacgtc agggcgataa
    22681 gattcggcaa cgccgccccc agcccgacac cggtcatcag tcgcgcaaag accagtgagg
    22741 ggaaatccca ggcaatcgcc gttgccagtg agaacaaacc aaacagcgca actgagctaa
    22801 ttaaaatacg cttgcgacca taacggtccg ccagcattcc gccaaccaac gcgccgggta
    22861 gcaaaccgag tattccggcg ctgaatatcc agcccatttg cattttatcg agtgcgaaag
    22921 cctgggcgat gccacccgcc gcaatgccag ccgcctgaag atccagtcct tccatcagag
    22981 cgaccaaaaa acaaagcccg atggtcagca tcaggcggga tgaagatgat gaaggggtac
    23041 gagtcgacat aagatgaacc tcattgcgag tgcaggtgaa tcgcaactga tacattgcca
    23101 gccgcgtaat cagaactatt taatgactgc ctacgggcga aatgtaggcc ggataaggcg
    23161 ttcacgccgc atccggcagt cgtgctggga tgggcataca aaatgcacgt tctcggcagt
    23221 caatattatt tgttgttgcg cagatccagc gccacgtcga cgatcatatc ctcctggccg
    23281 ccaaccatcc ggcgtttgcc cagctcaacg agaatatcca ccgcgcttaa gccataacgc
    23341 gccgccgccg tttcacagtg acgcaggaag ctggagtaaa caccagcgta tcccag
//
LOCUS       DAZFFE010000077        23136 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFFE010000077 DAZFFE010000000
VERSION     DAZFFE010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.077"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(116..832)
                     /gene="sdhB"
                     /locus_tag="ACHJ69_003204"
     CDS             complement(116..832)
                     /gene="sdhB"
                     /locus_tag="ACHJ69_003204"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO5016134.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(848..2614)
                     /gene="sdhA"
                     /locus_tag="ACHJ69_003205"
     CDS             complement(848..2614)
                     /gene="sdhA"
                     /locus_tag="ACHJ69_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO5016135.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVIESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(2614..2961)
                     /gene="sdhD"
                     /locus_tag="ACHJ69_003206"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(2614..2961)
                     /gene="sdhD"
                     /locus_tag="ACHJ69_003206"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO5016136.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(2955..3359)
                     /gene="sdhC"
                     /locus_tag="ACHJ69_003207"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(2955..3359)
                     /gene="sdhC"
                     /locus_tag="ACHJ69_003207"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO5016137.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPVTAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQAFAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            3740..3847
                     /locus_tag="ACHJ69_003208"
     CDS             3740..3847
                     /locus_tag="ACHJ69_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016138.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            4053..5336
                     /gene="gltA"
                     /locus_tag="ACHJ69_003209"
     CDS             4053..5336
                     /gene="gltA"
                     /locus_tag="ACHJ69_003209"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO5016139.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            5464..5856
                     /locus_tag="ACHJ69_003210"
                     /pseudo
     CDS             5464..5856
                     /locus_tag="ACHJ69_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            6008..7054
                     /gene="abrB"
                     /locus_tag="ACHJ69_003211"
     CDS             6008..7054
                     /gene="abrB"
                     /locus_tag="ACHJ69_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO5016140.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLALHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(7051..7842)
                     /gene="nei"
                     /locus_tag="ACHJ69_003212"
     CDS             complement(7051..7842)
                     /gene="nei"
                     /locus_tag="ACHJ69_003212"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO5016141.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKSYQSRLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRLSYATRGQVDE
                     NKYHGALFRFKVFHRDGEPCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7878..8612)
                     /gene="pxpA"
                     /locus_tag="ACHJ69_003213"
     CDS             complement(7878..8612)
                     /gene="pxpA"
                     /locus_tag="ACHJ69_003213"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO5016142.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVFAQTLYQIGALAAITRAQGGVMCH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGERYGLVTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWAMVTAQTVCLH
                     GDGEHALAFARRLRATFAEKGIVVAA"
     gene            complement(8602..9534)
                     /gene="pxpC"
                     /locus_tag="ACHJ69_003214"
     CDS             complement(8602..9534)
                     /gene="pxpC"
                     /locus_tag="ACHJ69_003214"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO5016143.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMRAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLRDGDRLPIGKAKRDFM
                     EAQGVKQRLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSNGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(9528..10184)
                     /gene="pxpB"
                     /locus_tag="ACHJ69_003215"
     CDS             complement(9528..10184)
                     /gene="pxpB"
                     /locus_tag="ACHJ69_003215"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO5016144.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVNMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAAGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLHVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPERDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(10207..10950)
                     /gene="ybgI"
                     /locus_tag="ACHJ69_003216"
     CDS             complement(10207..10950)
                     /gene="ybgI"
                     /locus_tag="ACHJ69_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO5016145.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQDLLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            11221..12702
                     /gene="dtpD"
                     /locus_tag="ACHJ69_003217"
     CDS             11221..12702
                     /gene="dtpD"
                     /locus_tag="ACHJ69_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO5016146.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYSD
                     NHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGF
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(12744..14162)
                     /gene="phrB"
                     /locus_tag="ACHJ69_003218"
     CDS             complement(12744..14162)
                     /gene="phrB"
                     /locus_tag="ACHJ69_003218"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO5016147.1"
                     /translation="MTTHLVWFRQDLRQHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNVSPRQAELINTQLNALQNALAEKGIPLLFREVDDFVASVEIVKQVCAENRVTHLFY
                     NYQYEVNERARDVQAERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQVLEGGPGSVWLNEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKAVHEPWKWAEKTGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(14159..14668)
                     /gene="ybgA"
                     /locus_tag="ACHJ69_003219"
     CDS             complement(14159..14668)
                     /gene="ybgA"
                     /locus_tag="ACHJ69_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO5016148.1"
                     /translation="MNQQLFDDSTLIRIFALHELHNLKDRGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHRWQSLDDYYNQYRQRVIVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQSLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHAGVL"
     gene            complement(14769..14975)
                     /gene="ybfA"
                     /locus_tag="ACHJ69_003220"
     CDS             complement(14769..14975)
                     /gene="ybfA"
                     /locus_tag="ACHJ69_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO5016149.1"
                     /translation="MELYKEYPAWLIFLRRTCAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATSDFY"
     gene            15288..15377
                     /gene="kdpF"
                     /locus_tag="ACHJ69_003221"
     CDS             15288..15377
                     /gene="kdpF"
                     /locus_tag="ACHJ69_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO5016150.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            15377..17050
                     /gene="kdpA"
                     /locus_tag="ACHJ69_003222"
     CDS             15377..17050
                     /gene="kdpA"
                     /locus_tag="ACHJ69_003222"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO5016151.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFSALGVSDREMNWKQYLSAILGLNILGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     NTLGNAWVDLLRITLWVLTPVALLIALFFIQQGVLQNFLPYQAVTTIEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLALCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            17073..19121
                     /gene="kdpB"
                     /locus_tag="ACHJ69_003223"
     CDS             17073..19121
                     /gene="kdpB"
                     /locus_tag="ACHJ69_003223"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO5016152.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGVMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVEEKALA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGIDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            19130..19702
                     /gene="kdpC"
                     /locus_tag="ACHJ69_003224"
     CDS             19130..19702
                     /gene="kdpC"
                     /locus_tag="ACHJ69_003224"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO5016153.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPNASTNVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            19695..22379
                     /gene="kdpD"
                     /locus_tag="ACHJ69_003225"
     CDS             19695..22379
                     /gene="kdpD"
                     /locus_tag="ACHJ69_003225"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO5016154.1"
                     /translation="MNNEPLRPDPDRLLEQAAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLKGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLITL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDAPPARTLNNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSSQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGINAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            22376..23053
                     /gene="kdpE"
                     /locus_tag="ACHJ69_003226"
     CDS             22376..23053
                     /gene="kdpE"
                     /locus_tag="ACHJ69_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO5016155.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATTTPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
BASE COUNT         5242 a         6229 c         6065 g         5600 t
ORIGIN      
        1 gcgtcttatc aggcctacgg tttctgcatt tcttttgtag gccggataag gcgttcacgc
       61 cgcatccggc actggttgcc tgatgcgacg cttgcgcgtc ttatcaggcc taggtttacg
      121 cattacgttg caacaacatc gacttgatat ggccgatggc gcgcgtcggg ttcagcccct
      181 tcggacatac actgacgcag ttcatgatgc tgtgacagcg gaatacactg aatgcatcgc
      241 tcaaaccgtc gaggcggctg tcagtctcgg tatcacggct atcgatcagg aaacgatatg
      301 ccgctaacaa gcctgccggg ccgataaact tatcgggatt ccaccagaaa gacggacaag
      361 aggttgaaca acatgcgcag agaatacatt catacaaccc gtcgagtttt tcgcgctgct
      421 ctggcatctg taaatgctcg cgagctggcg gattttgtcc attattcaac aggtaaggct
      481 taattttctc atattgcgca tagaattgtc ccatgtctac caccaaatcg cggatcaccg
      541 gtaaacctgg cagcgggcga atcacaatct tcttgcccgg ctggttgagt gccgaaatcg
      601 gagtaataca ggccagacca ttcttaccgt tcatgttcag accgtcggaa ccgcacacac
      661 cttcacggca ggagcggcgg aacgacaggc tgggatcttt ctctttcagc tgaataagcg
      721 catccagcag catcatgtcg cgaccttctt ccgcttccag ggtgtaatcc tgcatacgcg
      781 gagcatcatc aacatccggg ttatagcgat aaattgaaaa ctcgagtctc attttcctgt
      841 ctccgcatta gtaagtacga atcttcggcg ggaatgccgg gcgcagtttc ggttccatgt
      901 tgacgcttcg gcgcgtcatg gattccgact ctggcagata cagggagtgg cacagccagt
      961 tttcatcatc gcgatccggg aagtcgaagc ggctatgcgc gccacggctt tcggtacgga
     1021 agttggcaga aacagccgtt gcatacgccg tttccatcag gttatccagt tccaggcact
     1081 caacgcgctg ggtgttgaac tcgctggaag tgtcatccag acgggcattt ttcagacgct
     1141 cgcggataac tttcaactgc tcaagccctt tcgccatcgc atcaccttca cggaagaccg
     1201 agaagttatg ctgcatacat tcttgcagtg ctttacggat cgccaccgga tcttcaccgt
     1261 tacggttatt gttccagcgg ttcaggcgat ccagagacgc ttctacatca gactcgctgg
     1321 catcgcgcag tgcgccctgc tcggcaatag actcttgcag atgcagacct gccgcacgac
     1381 caaatacgac caggtccagc agcgagttgc cgcccagacg gttagcgcca tgtaccgata
     1441 cacaagcgat ttcaccaacg gcaaacagcc ccggaacaac cacatcttcg cctttctcat
     1501 tcacagtcag cgcctgaccg gtcactttgg tcggaatacc gcccatcatg tagtgacagg
     1561 ttggaattac cggaatcggc tctttcaccg gatcaacgtg agcgaaggtg cgggagagtt
     1621 ccagaatacc cggcagacgg gattcgataa cttctttccc cagatggtca agtttcagtt
     1681 ttgcgtgtgg cccccacgga ccatcacagc cgcggccttc acggatttcg atcatgatgg
     1741 aacgcgccac cacgtcacgg cccgccaggt ctttggcgtt cggcgcataa cgctccataa
     1801 agcgttcgcc atgtttgttc agcagataac cgccttcacc acggcaacct tcggtgacca
     1861 gtacgcccgc accggcaata ccggtcgggt ggaactgcca catttccata tcctgtaccg
     1921 gtacgcctgc acggatagcc atgccgacac cgtcgccagt gttaatgtgg gcgttggtgg
     1981 tggactgata aatacgccct gccccgccag tcgccagcac tgtcgcgcga gctttaaagt
     2041 aaaccacttc accagtttcg atgcacagtg cggtacaacc gaccactgcg ccatcctggt
     2101 ttttcaccag atccaacgca taccactcgg agaaaatggt ggtgtggttt ttcaggttct
     2161 gctgataaag cgtgtgcagc agtgcgtgac cggtacggtc ggcagccgcc gcagtacgtg
     2221 ccgcctgctc gccgccgaag tttttcgact gaccgccaaa cggacgttga tagatacgac
     2281 catcatcaag acgcgagaac ggcaggccca tatgttccag ttccagaatc gcttccggcc
     2341 cggttttaca catatattca atcgcgtcct ggtcaccgat atagtccgac ccttttacgg
     2401 tgtcgtacat atgccattcc cagttatctt catgggtatt acccagcgca acggtaatac
     2461 caccttgcgc agaaacggta tgggaacggg tcgggaagac tttagagagc agcgcacagg
     2521 tctggccact ctgggaaatt tgcagcgccg cgcgcatacc tgcgccgcca gcaccaatca
     2581 caactgcatc aaattctctg actggcaatt tcatcacaca ccccacacca caacgaatcc
     2641 ataaatcacg taaaccacca gtgcaacgac aatcaccagt tgcagcatca ggcgcaaggc
     2701 cagcggttta acgtagtcgg tcaacacctg ccacatgccg atccaggcat ggatcaagat
     2761 agaaaacagc gccagcaggg tgaacacttt ggtgaacgca gaggcgaaga aaccaatcca
     2821 gacttcatat gtcagctcgc cactggtagc gaaaaaaccg accatataaa tgatgtagag
     2881 cgtcaggacg atagcggtag cacgaacgag gatgaaatca tgtacgccat tgcgtcctaa
     2941 tgcggaggcg ttgcttacca tacgaggact cctgcgagaa gtgaaagcac gacagtaata
     3001 acaaaggaga ttttggcgga gcgtttaccc gcttcgaatg tttcttccag atagccaaaa
     3061 tccatcatca tgtggcgaat acctacgacg acgtgatatg ccagagcggt aaggatgccc
     3121 cacatgataa atttgacgaa gaagctgccc ataatcgcga aagcttgctc gaaaccttca
     3181 ggggaagaga ggctggtacc cagaagccac agcaggatgc ccactgcaac aaaggtgatc
     3241 acaccggaaa cgcgatggag aatggacgct atcgccgtga cggggaaccg gatggtctgt
     3301 aggtccagat taacaggtct ttgttttttc acatttctta tcatgaataa cgcccacatg
     3361 ctgttcttat tattccctgg ggactacggg cacagaggtt aactttctgt taccaggaga
     3421 cgtcgggatt tccttcctcc ggtctgcttg cgggtcagac agcgtccttt ctataactgc
     3481 gcgtcatgca aaacactgct tccagatgcg aaaacgacac gttacaacgc tgggtggctc
     3541 gggattgcag ggtgttccgg agacctggcg gcagtatagg ctgttcacaa aatcattaca
     3601 attaacctac atatagtttg tcgggtttta tccagaacag tgatctgcgt cacgataaca
     3661 acatttattt aatttttaat catctaattt gacaatcatt caacaaagtt gttacaaaca
     3721 ttaccaggaa aagcatataa tgcgtaaaag ttatgaagtc ggtatttcac ctaagattaa
     3781 cttatgtaac agtgtggaag tattgaccaa ttcattcggg acagttatta gtggtagaca
     3841 agtttaataa ttcggattgc taagtacttg attcgccatt tattcgtcat caatagatcc
     3901 tttacctgca agcgcccaga gctctgtacc caggttttcc cctctttcac agagcggcga
     3961 gccaaataac aaacgggtaa agccaggttg atgtgcgaag gcaaatttaa gttccggcag
     4021 tcttacgcaa taaggcgcta aggagacctt aaatggctga tacaaaagca aaactcaccc
     4081 tcaacgggga cacagctgtt gaactggatg tgctgaaagg cacgctgggt caagatgtta
     4141 ttgatatccg tactctcggt tcaaaaggtg tgttcacctt tgacccaggc ttcacttcaa
     4201 ccgcatcctg cgaatctaaa attactttta ttgatggtga tgaaggtatt ttgctgcacc
     4261 gcggtttccc gatcgatcag ctggcgaccg attctaacta cctggaagtt tgttacatcc
     4321 tgctgaatgg tgaaaaaccg actcaggaac agtatgacga atttaaaact acggtgaccc
     4381 gtcataccat gatccacgag cagattaccc gtctgttcca tgctttccgt cgcgactcgc
     4441 atccaatggc agtcatgtgt ggtattaccg gcgcgctggc agcgttctat cacgactcgc
     4501 tggatgttaa caatcctcgt catcgtgaaa ttgccgcgtt ccgcctgctg tcaaaaatgc
     4561 cgaccatggc cgcgatgtgt tacaagtatt ccattggtca gccatttgtt tacccgcgca
     4621 acgatctctc ctacgccggt aacttcctga atatgatgtt ctccacgccg tgcgaaccgt
     4681 atgaagttaa tccgattctg gaacgtgcta tggaccgtat tctgatcctg cacgctgacc
     4741 atgaacagaa cgcctccacc tccaccgtgc gtaccgctgg ctcttcgggt gcgaacccgt
     4801 ttgcctgtat cgctgcaggt attgcttcac tgtggggacc tgcgcacggt ggtgctaacg
     4861 aagcggcgct gaaaatgctg gaagaaatca gctccgttaa acacattccg gaatttgttc
     4921 gtcgtgcgaa agacaaaaat gattctttcc gcctgatggg cttcggtcac cgcgtgtaca
     4981 aaaattacga cccgcgcgcc accgtaatgc gtgaaacctg ccatgaagtt ctgaaagagc
     5041 tgggtaccaa agatgacctg ctggaagtgg ctatggagct ggaaaacatc gcgctgaacg
     5101 acccgtactt tatcgagaaa aaactgtacc cgaacgtcga tttctactct ggtatcatcc
     5161 tgaaagcgat gggtattccg tcttctatgt tcaccgtgat cttcgctatg gcacgtacgg
     5221 ttggctggat cgcccactgg agcgaaatgc acagtgacgg tatgaagatt gcccgtccgc
     5281 gtcagctgta tacaggatat gaaaaacgcg actttaaaag cgatatcaag cgttaatggt
     5341 tgattgctaa gccgttcact ttccggaccc gcctttaata gagcgggttc tttttatatg
     5401 gctgaacata aaaattgtaa aaattaaacc agatgcaaat acccattaat aaggtgaaaa
     5461 aatatttcac cggaaacact ggtggttgat tttggtaaat ttaacttact ggatattcgc
     5521 cgccacacaa tgtcgaaaac attcagtatc aagacaacaa aatcacaaaa tgaccagtgt
     5581 accgacggat ttaaggtcag ctcctcgttt tataccgaaa aaacgttggt tgaagaagat
     5641 aaagcattgt tgattggtaa tggtctgaag ctacgtatac tggatgaaaa cgcctcgcct
     5701 tatacattca ataaatatgc tgaatacgcc gatttcacca gtgatatgct gatctacgaa
     5761 aaaacctata cggctgaatt atcgtccaca cctggcactc ccatcgcggc gggtcccttc
     5821 gatacagtgg tactttttaa gattaactat aactgattta acacgacgcc cttttcagcc
     5881 ggaaagggca acgtattcag attattgctg attttcccct tttccttgat taccgttatt
     5941 ttttccagac ccagctttct ttatgctgta tcgatacacc aaagataaaa taatgaggaa
     6001 gcgaagaatg cctgtattgc agtggggaat gttatgtgtt ctgtcacttc tcctttctat
     6061 tggcttcctc gctttacatc tcccggcggc actcctgctt ggcccgatga tcgccgggat
     6121 catcttcagt atgcgcggag tcactctgca acttccccgc tccgcttttc tcgctgccca
     6181 agccattctt ggctgcatga ttgcacaaaa cctcactggt tcgattctca ccacgctggc
     6241 cgtcaactgg ccgatcgtgc ttgcgatttt actggtgacg ctgctttcca gcgccatcgt
     6301 gggttggtta ttggtgcgct atagctcact gcctggaaat accggggcct ggggttcttc
     6361 tcctggcggc gcggcggcaa tggtcgcaat ggcgcaagat tacggcgcag atattcgcct
     6421 ggtggcgttt atgcaatatc tgcgagtgct gttcgtcgca ggagccgctg ttctggtcac
     6481 ccgcatgatg ctgggcgata acgctgaagc ggttaaccag cagatcgtct ggttcccgcc
     6541 agtgagcatt aatctcctgc ttacgatttt gctcgcggtc gttgccggta cggcgggatg
     6601 cttgctacgt cttccttccg gcacgatgct catcccgatg ctggcgggcg cagtgctcca
     6661 gtctggtcag ctcatcacga ttgaactccc ggaatggctg ctggcgatgg catatatggc
     6721 aattggctgg cggattggtc ttggtttcga taagcaaata ttactgcggg cattgcgccc
     6781 actgccacaa atcctgctgt cgatttttgc tctgctggct atttgtgcgg gtatggcgtg
     6841 ggggctgacc cggtttatgc atattgattt tatgaccgcc tacctcgcca ccagccccgg
     6901 cgggcttgat accgtggcgg tcatcgccgc agggagcaat gccgatatgg cgctcatcat
     6961 ggcgatgcaa accctgcgcc tgttcagtat tttgctgacg gggcctgcca tcgcacggtt
     7021 tatttcaacc tatgcgccga agcgttcggc ctagtgctgg cagcctgggc accagtaaaa
     7081 cgggcgcgat gacagcgtgg ttttctcaat gatgccgcca cagcgttcgc aaggttcgcc
     7141 atcacgatga aaaaccttaa agcgaaacag cgccccatga tatttgtttt catccacctg
     7201 gccccgcgta gcgtaagaaa gtcgcggaat atccagtaac gcgtgtgcga gtgcatccag
     7261 ttgcgccgca ttgagatctt tcgctttatg atttccggtc aacccaacct gccagaggat
     7321 ctccacccgc aaataattgc caagtcctgc cagaaacgcc tgatcgagca gtaatccagc
     7381 aaactggcgg ttacgaaagc gcggcgacaa taatcgttcc tttaccacct ccggcgtcag
     7441 attcggatcc agcacatcgg gaccaacgcg ttgtaaaaac ggatgcgtgg tcagttgttc
     7501 cggagtcaac atctcaatat ccgaggcgct ataaagcaga atggttttgt cagccgtttg
     7561 cagttttacc cgcagtactc gcgtggtctg cggctcttcg ccggtatcaa ccacgcgcca
     7621 gacgccgtaa agctgattat ggctgtagag cgttaagtcg ttggaaaaat gagttaacaa
     7681 cgccttacca cgcgtttcca catgggtaac gtgttgaccg ataagtcgtg attgataaga
     7741 ttttaactgc gggaaggcaa accagacatc agttagtggt ttgcctttga tcgccgcctc
     7801 caggttatcc gctgcacggc ggatctccgg gccttcaggc atggtgatat ccttttttca
     7861 ttctttatta agcggggtta tgctgcgaca acaatcccct tttcagcaaa tgtagctcgt
     7921 aatcggcggg cgaacgccag tgcgtgctcg ccatcgccat gtaggcagac cgtttgcgcc
     7981 gtgaccattg cccattcgcc ggtgatactt ttgactctgc cgtgttgcac catctccaac
     8041 gtttgcgcca gcgcctgttc ttcgttttca atcaacgcgc ctggctggct tcgcggcacc
     8101 agcgaaccgt ctgcctgata accacgatcg gcaaacactt cctcgcgcgt taccaggcca
     8161 tatcgttcgc ctgcacgaat cagctcgctt cctgccagcc cgacgagaat caatgccgga
     8221 tcgcaagcgt ataccgctct ggcgatggcg tctgccagtt gtgcctcttt cgccgcctgg
     8281 ttgtataaca tgccgtgcgg tttgacatga cacattacgc cgccttgcgc acgggtaatc
     8341 gctgccagcg cgccaatttg atacagcgtc tgggcgaaaa cagtttctgg cggcagctgc
     8401 atggcgctgc gaccaaaatt ttccctgtcg ggaaaactcg ggtgcgcgcc aatcgcgaca
     8461 ccgtttttta ttgcttcacg cacgcaagcc tgcatggttt gcgcatcgcc tgcatgaaat
     8521 ccacaggcaa tattggcaga ggaaaccagc gttaatagct ctgcgtcgct ggcgcagcct
     8581 tcgcccagat cggcgttcag gtcaattttc attgtgcagc cgccatgcta attgttcgaa
     8641 ataacgttgt tgatcttgcc gtgctttcag tgcctcttcc agtgaacact ggacaaaatg
     8701 aatcggctga ccgagcggaa tttgcgccag atggtacata tcagcctcaa tgatacaggc
     8761 aatacgcggg taaccaccgg tggtttgcgc atcgttcatc aacacaatgg gctgcccgtt
     8821 atgcggcacc tgcaccacac ctggtaacaa accgttagat aacagttcgc gatcggtggt
     8881 gcgttttaaa atttgcccct gtaagcgata gcccatccgg ttactttgcg agctaagctg
     8941 ccagggcgaa cgccagaatg catcctgcga ggcgcgatcg aactcatgat attccggccc
     9001 cggcaaggcg cgaatgcggt tgccccacag ccgctgttta acgccctgcg cttccataaa
     9061 atcacgcttc gctttgccaa tcgggagtcg gtcaccatcc ctcagtaaac ggccttccag
     9121 cccgccaatc cccactttga gatcggtgct gcatgatccc attaccggcg gaacatcaat
     9181 accacccgcg accgccagat aactgcgcat cccgtgctgc gggcgtttaa gcgttaaacg
     9241 ctggcctgct ctcattggca atcgccagcc ggtccagacg gcgttatcat ccagccgcgc
     9301 ttcgcaaccg gcaccggtca gggcaaacca cccatcagtt tcgaactcaa ccgttaactg
     9361 accgagcgtg atctccagcg cgggggcgtt ggcgtcatta cccaccagca ggttagcaat
     9421 gcgcagcgca ggcatatcca gtgcgccgca gtggctgata cccgactggc gaaaaccgtg
     9481 acgaccgcca tcctgcacag tggtatacat gcccgcacga ataatcttca gcatactccc
     9541 tccttctggg gtacaaagcg cacgctgtct cccggacgta ataagatggg ttcgtcacgc
     9601 tccggatcaa acaggctgag tgaggtatga ccaatcaact gccagccacc cggcgttgcc
     9661 agcggataaa caccggtctg cggcccgccg atcccgacag aacctgctgg aacgtgcaag
     9721 cgcggttcag cgcgccgtgg cgtgtgtagt tgttccggca aactcccgag atacgggaag
     9781 cccggttgaa aacctaaaaa ccagaccacg tattccacgg aggagtgcaa ttcaacaacc
     9841 tgtttttcgc tcaatccaca atgcgccgcg accaccgcca aatccggtcc ggctgcgcca
     9901 ccgtaaacca ccggaatttc aataaagcga gactccggct ccagcgcctc actctcctcc
     9961 caccagcgtt gcaaacgctc tatggcatcc agcgccagcg actcaggatt acgcaaaatc
    10021 actgtgatat tgttcatgcc aggaatggct tcaaccacat tcggcatatt caccagacgc
    10081 tgtgccagtc gccagatccg tttctggcta gccagcgtca ccggcggttc cagttccagc
    10141 actaccgccg tttcacctat cagataacaa cgcgctcgtt gcactttcga tccctctgat
    10201 tattcgttat gcaggattag gaatatcaat aaaggtcaca tcaagatcgg tattttcatt
    10261 cagccactcg ctcaatgcgc gaataccacc acgttcagtg gcatggtgac ctgcagcata
    10321 aaaatgcaat ccctgctcgc gggctgaatg aatggtctgt tcagaaactt cgccagtaat
    10381 aaaagcatcc acgccaaaac gcgcggcgct atcgataaaa ctttgcccgc cgcccgtgca
    10441 ccaggcgacg cgctggacca cctcaggtcc ggtatcgcca caccataacg gcttacgtcc
    10501 cagacgcgct tcaatccagg aggccagttc cagtcccggc acaggcatgg tcagttcgcc
    10561 ccacggcacc aacggctcaa tttcgcccat gaccgtgatc cccagtaacg ccgccagctg
    10621 tgcgttattg cccagctcag gatgtgcgtc aagcggcaaa tgccagccat acaggttgat
    10681 atcattcgcc aacaatgttt ttaagcggtt acgcttcatt ccgcgaatga ccggagattc
    10741 gcctttccag aagtagccat gatgcacaat gaccgcatcc gcgcccagac gcactgcctc
    10801 atcgagcaaa tcctggctgg cagtgacacc ggtaacaatt ttttgcaccg tctctttgcc
    10861 ttccacctgc aaaccattcg gcgcgtaatc actaatcgcc gcgctgttca gtttttcgtt
    10921 aatcagttgt tccagttcgg tgtttttcat cttcgcgcct tccgtattct gatagctaca
    10981 ccgttaacat agcgttacct cgtctgttcg tcgatagcca atcttttgac atattaagaa
    11041 acaaacccga cgccaaaaga cgcaaattgt ttatatcatc acgcttcagc taatgtatat
    11101 ttatgcaatc agacagcata ttgcataaat aatcagatct gtatcaaagt tttaacttta
    11161 aggaaacaca agcacgccgc gcaactatta cgtgacgttg cagtcaattg tggaattatt
    11221 atgaataaac acgcatcaca accgcgcgct atttactatg tcgttgcgct gcaaatctgg
    11281 gaatatttta gcttttacgg catgcgtgcc ctgctgatcc tctatctcac caatcaacta
    11341 aaatatagcg ataatcacgc ctacgagtta tttagcgcct actgttcgct ggtatatgtc
    11401 acgccaatcc tcggtggctt tttggcggat aaagttctcg gcaatcgcat ggcggtgatg
    11461 ctgggggcgt tgttgatggc gatcggtcat gtggtgctgg gtgccagtga gatccatccg
    11521 tcattcctct atctgtccct ggcgattatc gtctgcggct atggtctgtt taaatccaac
    11581 gtcagttgcc tgctcggcga gctgtatgag ccaaccgatc cacgtcgtga tggcggtttc
    11641 tcgctgatgt atgcggcggg taacgtgggg tctattatcg cacctatcgc ctgtggtttc
    11701 gcccaggaag agtacagttg ggcgatgggc tttggcctgg cggcggtggg tatgatcgcg
    11761 ggtctggtca ttttcttatg tggcaatcgt catttcacac atacccgcgg cgttaacaaa
    11821 aaggtactgc gtgcgacaaa ctttctcctg cccaactggg gatggctact ggttctgctg
    11881 gtggcaacgc ctgcgctgat taccgtgttg ttctggaaag agtggtcggt atacgccttg
    11941 attgtcgcga cgatcattgg ccttggggtt ctggcaaaaa tttatcgcaa agcagaaaac
    12001 caaaaacagc gtaaggagct ggggctgatt gttaccctca ccttcttcag catgttgttc
    12061 tgggcattcg cacaacaggg gggtagctcg attagccttt atatcgaccg cttcgttaac
    12121 cgcgatatgt ttggttatac cgttccgact gctatgttcc agtcgattaa tgccttcgca
    12181 gttatgctgt gcggtgtgtt cctggcgtgg gtggttaaag aaagcgtcgc aggtaatcgt
    12241 accgtgcgca tctggggaaa atttgctctt ggccttggcc tgatgagcgc cggattctgc
    12301 attctgacct taagcgcccg ctggtccgca atgtatggtc actcttctct gccactgatg
    12361 gtgttaggcc tggcggtgat gggctttgcg gaactgttta tcgacccggt tgctatgtcg
    12421 cagattacgc gcattgaaat ccccggcgtg accggcgtat taaccggcat ttatatgctg
    12481 ctttccggcg cgattgcgaa ctatctggcg ggcgtgattg ccgatcagac atcgcaggct
    12541 tcgtttgatg cttccggggc gatcaactac tccatcaatg catatattga agtatttgat
    12601 caaattacct ggggcgcact ggcgtgtgta ggactggtac tgatgatttg gctgtatcag
    12661 gcgcttaagt tcagaaaccg cgcgctggcg ctggagtctt aataagctag caccatgcct
    12721 gataaaacgc tcgctgcgtt ttatcaggct cctttcctcg cttcttcgta cgctgccagc
    12781 gttcgcagcc ttgcctcttt gtgatcgact atcggttgcg gataatccaa cgtcacacct
    12841 gttttctctg cccacttcca cggctcatgc accgctttcc ccggtacatc gcgcagctcc
    12901 ggtagccact ggcggataaa ttcgccttcg cggtcaaatt tctcgccctg agttgtcggg
    12961 ttgaaaatac gaaaatacgg cgctgcatca gtacccgtag aagcagccca ctgccagcca
    13021 ccgttattag ctgccaaatc accatcaatc aactgcgaca tgaaatatcg ctcgccttcg
    13081 cgccagtcga tcaacaaatc tttcaccaga aaactggctg taatcatccg tagccggtta
    13141 tgcatccagc cagtgctgtt aagctgacgc atggcagcat cgacaatcgg gtatcccgtt
    13201 ttgccctcct gccaggcctt taaatgggcg ggattgcttt gccactgtac acgatccgtc
    13261 caggcaataa acggacgatg tttacacagc gaaggataat acgtcatcaa atggcggtag
    13321 aactcgcgcc atatcagctc attaagccag acgctccccg gtccgccttc cagcacctgc
    13381 ggttgttccg ccaacaggcg atgtaaacac tggcgaggcg ataacccgcc cgttgccagg
    13441 ctggcggaca aacgactggt gccttccact gccggaaaat ctcgttgttg ctcatactct
    13501 ccggcaccgt tctggcaaaa ctggcgtaat tgcgcaatcg ccgctttttc ttccaccggg
    13561 aaatgcgcag tatcgaaaga ctgacgagga taattcagcg taatggatgg cgcgggctct
    13621 atcgatccgc tactacgaac ttttggtgca gcaacgcact ccggcatccc ttcccgcagc
    13681 cgtttcaacc atgcgttttt aaaaggcgta aagactttgt acatctcatg attgccagtc
    13741 atcaccgccc ccggcggcag gatcacgctg tcatcaaatc cttcacacac cacgttacgc
    13801 agcgtcctct cagcctgcac atcccgcgcc cgctcattca cctcatactg atagttataa
    13861 aacagatggg taacacgatt ttccgcgcac acctgtttaa ctatttcgac gctggcgaca
    13921 aagtcatcca cttcacggaa caataaagga ataccttttt ccgccagcgc gttttgcagc
    13981 gcattaagtt gtgtattgat aagttcagcc tgacgcggcg acacattatg cgccgcccac
    14041 tggcgtggtg tagcgatata caacgccagc acgcgcgcag acgaattgcg gcaggcggca
    14101 gccagcgcga gattatcgtg ctgacgtaaa tcctggcgaa accagaccag atgggtagtc
    14161 ataaaactcc tgcatggcgc aagttaatca cacgcggcca aagttcgaaa taacgctgcc
    14221 ctgaaagcca ggcgtcagga taaagcgcca tatagtgttt gatacgcatc agcggcgcaa
    14281 gaagcgattg ctcgccacgg cgataactgt cgataagcgc cgccagctgc tggcgttctg
    14341 tggaatcaat atgtgggcga aaatatccct gaacgtgcat caaaacgtta gtatgatcac
    14401 gccggttggc agggtgagaa agcaaaacaa tcacgcgttg gcggtactgg ttgtaatagt
    14461 catccagact ttgccaccgg tgaatatcag ccacaaacgg gccaagtttg cggtactccg
    14521 gctgagaatg cgccagaaag acgagtttat agcggctgtg ataatcgaga agcgccccgc
    14581 gcgttagtcc tcgatctttg agattgtgta actcatgcaa agcaaaaatg cggataaggg
    14641 tgctgtcatc aaaaagttgt tgattcatta ctgctcctga acggtttaca ggagtaagtg
    14701 tagacgccga aaaaagaaaa ccgccagcgt ttccaccggc ggttttcttt aaacgtcatg
    14761 tttggcaatc agtaaaaatc actggttgcc ttttcggcct gatccatcca caccggttta
    14821 tcgctggttt tcgaccagac gcggtgcagg tagctataaa agcgggcgcg gtctttccag
    14881 aaaagcatga aaggcagcgc cagaacgccc gctgcaaccg cacaagtacg gcgtaaaaag
    14941 ataagccatg caggatattc tttgtagagt tccatacttt tctccccctt ttgtatctac
    15001 ccggtgaata gcaccggaaa aatgaattcg tttatctgat gaaaatagta ccgctgtttg
    15061 tgtaatttta ctactcatcc gaccacttat ttttgcttat tgatggttta tttacattca
    15121 ccgtgtaatt aagttacata aaagttaaat taatactaag cattagttaa atcatggctt
    15181 ttgccatttt tatacttttt ttacaccacg cccgcagatt tttgcgaaat ctttgcagcc
    15241 agaactctac ccttccggta tcacttttag gccactggag gtgcactgtg agtgcaggcg
    15301 tgataaccgg cgtgttgctg gtgtttttat tactgggtta tctggtttat gccctgatca
    15361 atgcggaggc gttctgatgg ctgcgcaagg gttcttactg attgccacgt ttttactggt
    15421 attaatggtg ctggcgcgtc ccttaggtag cgggctggcg cggctgatta atgatatacc
    15481 tcttcctggt acgacgggcg ttgaacgcgt actctttagc gcgcttggcg tctctgaccg
    15541 cgagatgaac tggaagcagt atctctcagc cattcttggc ctgaatatac tggggctggc
    15601 ggtgctgttt tttatgttgc tcggtcagca ctatctgccg cttaatccac agcagttgcc
    15661 ggggctgtcg tgggatctgg cgctcaatac cgccgtcagc tttgtcacga ataccaactg
    15721 gcaatcttat agcggtgaaa ccacgttgag ctatttcagc cagatggcgg gcttaacggt
    15781 gcaaaacttt ctttctgccg ccagcgggat tgcggtgatt tttgcgctta ttcgcgcgtt
    15841 tacccgccag agcatgaaca cgctgggtaa tgcgtgggta gacctgttgc ggattacgtt
    15901 atgggtgtta acccccgtag cattgctgat cgcactgttt tttatacaac aaggtgtgct
    15961 gcaaaacttt ttgccttatc aggctgtgac gaccattgaa ggagcgcaac agctattacc
    16021 aatggggcca gtagcttctc aggaagcgat caaaatgctc ggaactaacg gcggtggctt
    16081 ttttaatgcc aactcgtcgc atccgtttga aaacccaacc gcactgacca acttcgtgca
    16141 aatgctggcg atcttcttga tcccaacagc gctgtgcttt gcctttggtg aagtggcggg
    16201 cgatcgtcgc caggggcgca tgttgctgtg ggcgatgtca gtgatttttg tcatctgcgt
    16261 aggcgtagtg atgtgggcag aagttcaggg taatcctcat ctgctggcgc tcggcgcgga
    16321 cagcagcatc aatatggaag gtaaagagag ccgtttcggc gtgctggtca gtagcctgtt
    16381 tgcggtcgtg actacggcgg cttcctgcgg cgcggtgatt gcgatgcatg attcgtttac
    16441 cgctcttggt ggcatggtgc cgatgtggtt gatgcaaatt ggtgaagtgg tattcggcgg
    16501 cgtcggttca ggcctttacg gcatgatgct gtttgtcctg ctggcggtgt ttattgccgg
    16561 gctgatgatt ggtcgtacac cggaatatct gggtaaaaaa atcgacgtac gcgagatgaa
    16621 actgactgca ctggcgattc tggtcacccc gacgctggtg ctgatgggcg cggcgctggc
    16681 aatgatgacc gacgccggac gtagcgccat gctcaaccct ggcccgcatg gttttagcga
    16741 agtgctgtat gccgtgtcat ccgccgctaa caacaacggc agcgcctttg ccggattaag
    16801 cgccaactct ccattctgga actgtttact ggcgttgtgc atgtttgtcg ggcgcttcgg
    16861 ggtgattatc ccggtgatgg caattgccgg ttcgctggtg agtaaaaaga gccaacccgc
    16921 cagctccggc acattgccaa cgcacggccc gctgtttgtt ggcctgttaa tcggcaccgt
    16981 gttgctggtt ggcgcactga cctttatccc tgccctggcg cttggtccgg tggcggaata
    17041 tctctcctga tgatattgag tgagcactga atatgagtcg taaacaactg gcgctattcg
    17101 aaccaacact tgtcgttcag gcgctgaaag aagcggtgaa aaaattaaac ccgcaggcgc
    17161 aatggcgcaa tccggtgatg tttatcgtct ggatcggcag tctgctgacc acctgtatta
    17221 gcatcgcgat ggcaagcggt gtgatgcctg gcaatgcgct gtttagcgcg gcaattagcg
    17281 gctggctgtg ggtcaccgta ctgttcgcta atttcgccga ggcgttggca gaaggccgca
    17341 gtaaagcgca ggccaacagt ctgaaagggg tgaaaaaaac cgcttttgct cgcaaactgc
    17401 gcgaaccgaa atatggcgct gcggcggaca aagttcctgc tgatcaactg cgtaaaggtg
    17461 acatcgtgct ggtagaagct ggcgatatta tcccctgcga tggtgaagtc attgaaggcg
    17521 gcgcatcggt cgatgaaagc gccatcaccg gggaatcggc tccggtgatc cgtgaatccg
    17581 gcggtgattt tgcctccgtc actggcggca cgcgtattct ttctgactgg ctggtgattg
    17641 agtgcagcgt taaccccggc gagacatttc tggatcggat gatcgcgatg gtggaaggtg
    17701 cgcagcgacg taaaacgccg aacgagattg ccctgaccat tctcctgatt gccctgacta
    17761 tcgtcttttt actggcaacc gccacgctgt ggccgttttc cgcgtggggc ggtaatgcag
    17821 tcagcgtaac ggtactggtg gcactgctgg tctgtctgat cccaaccact attggcggcc
    17881 tgttgtcagc gattggcgtc gccgggatga gtcggatgct gggcgcgaat gttattgcca
    17941 ccagcgggcg agcagttgaa gcagcgggtg atgttgacgt tctgctgctg gataaaaccg
    18001 gcaccatcac cctcggtaac cgtcaggcgt cagagtttat ccccgcacaa ggcgtggagg
    18061 aaaaagcgct ggccgacgcc gcacaactgg cttcgctggc tgatgaaacg ccggaaggcc
    18121 gcagtattgt gatcctcgcc aagcagcgtt ttaacctgcg cgagcgcgat gtgcagtcgc
    18181 tccatgccac ctttgtaccg tttactgcac aaagccggat gagcgggatc aacatcgaca
    18241 accgcatgat ccgtaaaggt tctgtcgatg ccattcgccg ccatgttgag gctaacggcg
    18301 gtcacttccc tgccgatgtt gatcaaaaag tcgatcaggt tgcgcgtcag ggagccacgc
    18361 cgctggtggt ggtggaaggt tctcgtgtgc tgggcgttat tgcgctgaaa gatatcgtca
    18421 aaggcggtat taaagagcgc ttcgcccagc tgcgtaaaat gggcattaaa acggtgatga
    18481 ttaccggcga taaccgcctg actgccgccg cgatagctgc ggaagcgggt atcgatgatt
    18541 ttctcgccga agcaacaccg gaggccaagc tggcactgat tcgtcagtat caggcggaag
    18601 gccgtttagt ggcgatgacc ggcgatggca ccaacgatgc tccggcgctg gcacaggccg
    18661 atgttgcggt ggcgatgaac tccggcaccc aggcggcgaa agaagcgggc aatatggtgg
    18721 atctcgactc caacccgacc aagttgatcg aggtagtgca cattggcaaa cagatgctga
    18781 tgacccgtgg ctcgttgacc accttcagca ttgccaacga tgtggcgaaa tacttcgcca
    18841 ttattccagc ggcgttcgcg gcaacgtatc cgcagttaaa tgcgctgaac atcatgcgcc
    18901 tgcattcgcc cgactccgcc attctcagtg cggtcatttt caacgccttg attatcgtct
    18961 ttttgattcc cctggcgtta aaaggcgtga gttataaacc gcttaccgct tctgccatgt
    19021 tgcgccgtaa cttatggatt tacggtctgg gtgggctgct ggtgccgttt attggtatca
    19081 aagtcattga tttactgcta accgtttgcg gtctggtgtg aggtttacca tgagtggatt
    19141 acgtccggca ttatcgacat ttctctttct gttattgatt actggcggcg tttacccgct
    19201 gctgaccacc gcactggggc aatggtggtt tccctggcag gccaacggtt cattgattcg
    19261 tgaaggtgat acggtgcgcg gttcggcatt aatcgggcaa aattttaccg gcaacggcta
    19321 ttttcatggt cgcccgtcgg caacagcaga aatgccctat aatccacagg cttctggcgg
    19381 gagcaacctg gcggtcagta acccggagct ggataaacag atagccgcac gcgttgctgc
    19441 attacgggct gctaacccga atgccagcac gaacgttccg gttgaactgg tgacggcgtc
    19501 agcaagcggg ctggacaata acatcacccc gcaagcggcg gcctggcaga tcccacgcgt
    19561 ggcgaaagcg cgtaatctca gcgttgaaca gctcacgcaa ctgatcgcaa aatacagcca
    19621 acaaccgctg gtgaaatata tcggccagcc ggtcgtcaac attgtcgaac tcaatctggc
    19681 actggataag cttgatgaat aacgaaccct tacgtcccga ccccgatcgt ctgctggaac
    19741 aagctgccgc gccgcatcgg gggaagctga aagttttctt cggtgcctgc gcaggcgtcg
    19801 ggaagacctg ggcgatgctg gcggaagccc agcgactgcg ggcgcaaggg ctggatattg
    19861 tggttggcgt ggtagaaacc cacggtcgga aagataccgc cgcaatgctg aaaggtctgg
    19921 ctgttctgcc gttaaaacgc caggcgtacc gtgggcggca catcagcgag tttgatctcg
    19981 atgccgccct cgcccgccgt ccggcactga tcttaatgga cgaactggcg cacagtaatg
    20041 cgccaggttc tcgtcatcct aaacgctggc aggacatcga agagctgctg gaagcaggca
    20101 ttgatgtttt cactaccgtc aacgttcagc atctggaaag cctgaacgat gtggtcagcg
    20161 gcgtcaccgg aattcaggta cgggagaccg tgcccgatcc tttttttgat gccgccgacg
    20221 acgtggtgct ggtggacttg cccccggacg atctgcgcca gcgactgaaa gaaggcaaag
    20281 tctatattgc cgggcaggcg gagcgcgcca ttgaacattt tttccgcaaa ggtaatctga
    20341 tcaccctgcg cgaactggca ctgcgccgta ctgccgatcg cgtagatgag caaatgcgcg
    20401 cctggcgggg gcatcctggc gaagagaaag tctggcacac acgcgacgcg atccttttat
    20461 gcatcgggca taacaccggc agtgagaaac tggtccgcgc ggcggcgcgg ttggcctcgc
    20521 gcctgggcag tgtctggcac gcggtgtatg ttgaaacccc tgccctgcat cgcttaccgg
    20581 agaaaaaacg tcgggcaatt ctcagcgcct tacgtctggc gcaggagctg ggcgcggaga
    20641 cggcaacact ttctgatcca gcggaagaaa aagcggtagt gcgttatgcc cgtgaacata
    20701 atctcggcaa gattattctc ggtcgcccgg cctcgcgccg ctggtggcgt cgggaaacat
    20761 ttgctgaccg actggcgcgt atcgcccccg atctcgatca ggtgctggtc gcacttgatg
    20821 ccccccccgc ccgcacgctt aataacgcgc cggatagccg ctcttttaaa gacaagtggc
    20881 gggtacagat tcagggatgc gtggttgccg ccgcgttatg cgccgttatc accttaattg
    20941 ccatgcagtg gctgatggcg tttgatgccg ccaacctggt gatgctgtat ctgcttggcg
    21001 tggttgtggt ggcactgttt tatggccgct ggccttcggt ggttgcgacc gtcattaatg
    21061 tggtgagttt cgatctcttt tttatcgccc cacgcggcac gctcgccgtc tctgatgtgc
    21121 aatatctgct gaccttcgcg gtgatgttaa ccgtcgggct ggtgatcggg aaccttactg
    21181 ctggcgtgcg ttatcaggcg cgggtagccc gttaccgcga gcaacgcaca cggcacttat
    21241 atgaaatgtc caaagctctg gctgtgggcc gtagttcgca ggatatcgcc gccaccagcg
    21301 aacagtttat tgcctccacg tttcatgccc gtagccaggt gttgctgcct gatgacaacg
    21361 gtaaattgca gcctttaaca catccgcaag gaatgacgcc gtgggacgat gccatcgcgc
    21421 agtggagttt tgataaaggc ctgcctgcgg gcgcgggcac cgacacgtta cccggtgtac
    21481 cgtaccagat tttgccgcta aaaagcggcg agaaaaccta cggactggtg gtggtggaac
    21541 cggggaatct gcgccagttg atgatcccgg aacagcagcg cctgctggag acgtttacgc
    21601 tgttagtcgc caatgccctt gagcgactga cactgaccgc cagcgaagaa caggcgcgga
    21661 tggcaagcga acgtgaacag atccgcaacg ccctgctggc ggcgctttcg catgatttac
    21721 gcacgccgct tacggtgctg ttcggtcagg cagaaatctt aacgctcgat ctggcaagcg
    21781 aaggatcgcc ccacgcccgc caggccagcg agattcgtca gcatgtgctg aacaccaccc
    21841 gactggtgaa taatctactg gatatggcgc gaattcagtc cggcggcttt aatttgaaga
    21901 aagagtggtt aacgctggaa gaagtggtcg gcagcgcgct gcaaatgctg gaaccgggtt
    21961 tatcgtcgcc catcaatctt tctctgccag agccgctgac cttaatccac gttgacgggc
    22021 cactctttga acgggtgctg attaacctgc tggagaacgc ggtgaaatat gcgggggcgc
    22081 aggccgaaat tggtatcaat gcccacgttg agggcgaaaa tctacaactg gatgtctggg
    22141 ataacggccc cggtcttcca ccaggccagg agcagacgat atttgataag tttgctcgcg
    22201 ggaataaaga gtcggcggta ccgggtgtag ggcttgggct ggcaatttgt cgggcgatag
    22261 tggatgtaca cggcggcacc attaccgcat tcaaccgacc ggaaggtggt gcctgttttc
    22321 gtgttacact tccccagcaa acagcccctg aacttgaaga atttcatgag gatatgtgac
    22381 aaacgttctg attgttgaag atgaacaggc tattcgtcgc tttctgcgca cggcgctgga
    22441 gggcgacggt atgcgcgtct ttgaggccga aacgctgcaa cgcggcttgc tggaagcggc
    22501 aacccgtaag ccagatttga ttattctcga tctcggcctg cccgacggtg atgggattga
    22561 gtttatccgc gacctgcgcc agtggagcgc ggtgccggtg attgtgcttt ccgcacgcag
    22621 cgaagagagc gataaaatcg ccgcgctgga tgccggagcg gatgattatc tgagtaagcc
    22681 gtttggcatt ggcgaattgc aggcccgtct gcgcgtcgca ttacgccgcc actccgccac
    22741 caccacgccc gatccgctgg tgaaattttc tgatgttacc gtcgatttag ccgcccgcgt
    22801 tatccaccgc ggcgaggaag aggtgcatct cacaccgatt gagttccgcc tgctggccgt
    22861 gctgctcaac aacgccggaa aagtgctcac ccagcgccag ttactcaatc aggtgtgggg
    22921 accaaacgcg gtcgaacaca gccactattt gcgtatttat atggggcatc tgcgacaaaa
    22981 actggagcaa gatcccgccc gcccccgcca ttttattact gaaaccggta ttgggtatcg
    23041 gtttatgcca tgaattatta ttcaataaat acgcttaaat aattattaat ttattattaa
    23101 acctgccccg aaaattatta tttggcaggt ttaatt
//
LOCUS       DAZFFE010000078        22895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFFE010000078 DAZFFE010000000
VERSION     DAZFFE010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.078"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..114)
                     /locus_tag="ACHJ69_003227"
     CDS             complement(<1..114)
                     /locus_tag="ACHJ69_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein"
                     /protein_id="HFO5016156.1"
                     /translation="MSRHATDWAWETDPGSSSLKLILLSMADRADEYNLCYP"
     gene            complement(130..330)
                     /locus_tag="ACHJ69_003228"
     CDS             complement(130..330)
                     /locus_tag="ACHJ69_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016157.1"
                     /translation="MEIITRIDAAKRGLKRYYTGKTCKHGHDSERWVYNGHCVECTME
                     SNRRIRAEIKQIMINSSPQHSS"
     gene            complement(481..711)
                     /locus_tag="ACHJ69_003229"
     CDS             complement(481..711)
                     /locus_tag="ACHJ69_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016158.1"
                     /translation="MSDNGHFDFKKHWLALTPDEREAFAQEAGTTSHYIQTHLTGKRK
                     MPGKVLMNGLFKACKTRQWLRSKAELAYFFYS"
     gene            866..1450
                     /locus_tag="ACHJ69_003230"
     CDS             866..1450
                     /locus_tag="ACHJ69_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5016159.1"
                     /translation="MKSIQDVRRQNLNDLIDREFNGVQTRMAEKLGTQANLVNRWALG
                     KKVIGDQVARKIEAAANKPRNWLDIDRSLSQEGFQPVGPSDIGQLAAHNLERWMSESR
                     DLSTQGKLHRASGVAQVTISRLLNNEVSVSISTLENVASAFGRHGYELLIHPHDPATI
                     NYDRSRYALLPETEKAKIESYIEFVINQNEKNKQ"
     gene            1604..1756
                     /locus_tag="ACHJ69_003231"
     CDS             1604..1756
                     /locus_tag="ACHJ69_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016160.1"
                     /translation="MQWKIINGWYCVTACGFMSWKFRTLQEGIKWAFVSKEARDVAND
                     NEIWEG"
     gene            1759..2058
                     /locus_tag="ACHJ69_003232"
     CDS             1759..2058
                     /locus_tag="ACHJ69_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016161.1"
                     /translation="MNVNQQKNLQKIMLAFDKDYRLSEQLYDRQVELIESIRLHQLAS
                     TFDVVTGKGVRQEVLEAAKDSPEFEELMDAYRREAMAIIARWDLADQLDGQRDAA"
     gene            2055..2876
                     /locus_tag="ACHJ69_003233"
     CDS             2055..2876
                     /locus_tag="ACHJ69_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFO5016162.1"
                     /translation="MKPGIYFDISNEDYHAGDGVSKSQLDMVAKNPALLKWVQAAPED
                     EEKKSALDMGTALHCLLLEPGEFDKRFIVSPKFDRRTKQGKADEEAFLRDVADMGITV
                     LDVEQWRKLELMRDSAMAHPAARWMLEAPGYCEASMYWNDEETGELCRIRPDKWLNEH
                     NVIVDVKKVADMDRFARHIEEFRYHVQDAMYREGAMRVTGQPHGFFFLAVSESIDCGR
                     YPVRVFELDAPDVDAGHALFRRDLNTYHECRINDEWGGVEIIKRPDWARKQDMYV"
     gene            2873..3754
                     /locus_tag="ACHJ69_003234"
     CDS             2873..3754
                     /locus_tag="ACHJ69_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecT family recombinase"
                     /protein_id="HFO5016163.1"
                     /translation="MSNDIAITSQPGATVGTAAAIFSPEGMNQLVRFAELMSQSKATV
                     PKHLEGKPADCLAVTMQAAQWGMNPFAVAQKTHVVNGTLGYEAQLVNAVVSSSSLLAT
                     RLNYRWSGDWSNVNGKTDKSPNLTVTVSAVLKGEAEPRELTISMAQAGVRNSPLWEQD
                     PRQQLAYLCTKRWARLHAPDVLLGVYTPDELQETAPRVERDITPQTTTAAGMNSLINA
                     KTVKKPDEQTRKADSRDPEEMLMAFTSAAMNYSTVSELDKAYKYIAQKLSDDDELLAK
                     ATDVYSVRREELNETSM"
     gene            3804..4052
                     /locus_tag="ACHJ69_003235"
     CDS             3804..4052
                     /locus_tag="ACHJ69_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009485476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5016164.1"
                     /translation="MKGALGKKELLAVVPLSWSTIDRMERAGEFPKRWYITDKRCAWN
                     RDEVERWLDERQAASPAEFQGKKPPVQQRVYRPVSNAA"
     gene            4210..4461
                     /locus_tag="ACHJ69_003236"
     CDS             4210..4461
                     /locus_tag="ACHJ69_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5016165.1"
                     /translation="MRSPSKIVAVTPAAIEIEKRAIEREKKGQFRIAAHLWLQCMDVA
                     SGDVERARIAVRRDQCITKGNGLRRGDYSGIGCCGVVYD"
     gene            4454..5104
                     /locus_tag="ACHJ69_003237"
     CDS             4454..5104
                     /locus_tag="ACHJ69_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFO5016166.1"
                     /translation="MTKKYTLIYADPPWVYRDKAADGNRGAGFKYPVMSVLDICRLPV
                     WDLADENCLLAMWWVPTQPLEALKVVEAWGFRLMTMKGFTWIKCGSRQPDKLVMGMGH
                     MTRANSEDCLFAVKGKLPTRINAGIVQSFTAPRLEHSRKPDIVREKLVQLLGDVSRIE
                     LFARQSSHGFDVWGNQCEDPAVQLHPGYALDIGGLTNAFSNAPLSPTDNQGRERAA"
     gene            5101..5295
                     /locus_tag="ACHJ69_003238"
     CDS             5101..5295
                     /locus_tag="ACHJ69_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO5016167.1"
                     /translation="MNRASPADLRKCLETANMLAHSGIRFVPIPAVTDAEFATLSAIF
                     ENKIESLAAEAKMEENQQNY"
     gene            complement(5299..6549)
                     /locus_tag="ACHJ69_003239"
     CDS             complement(5299..6549)
                     /locus_tag="ACHJ69_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5016168.1"
                     /translation="MLTDTKLKNLKPQDKLYKVSDRDGLYVAVLTSGTVSFRYDYRIN
                     GRRETLVIGQYGRDGISLAEAREELIAAKKLLKAGQSPAAAKRDGIKKIRGAETFAVH
                     TDSYMKHVILADSTRAMKQAVIDRDILPVLGNKMMAEITTSMVRDLCDRIVERGGRAT
                     AVQAREIISSVYRHANDRGHGLFNPAADIKPSSIAIFKPRERTLTPEEIGLFFRTLDA
                     IGAMGTMKMALKLVLITMVRKGEFTNATWDEIDFKKWTWTIPSDRMKGSRAHVIYLPK
                     QAQDILVGLQMCAGGSEYLVPGRYNFRKPLSNAALNSLIDRTVKIINEDGEHIQGFTV
                     HDMRRTASTLLHEAGYPSDWIEKALAHEHKGVRAVYNKAEYARQRAYMLQQWADMIDS
                     WINGEHTDLIPFSPSKFEKWMAGE"
     gene            complement(6742..8319)
                     /gene="guaA"
                     /locus_tag="ACHJ69_003240"
     CDS             complement(6742..8319)
                     /gene="guaA"
                     /locus_tag="ACHJ69_003240"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO5016169.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(8388..9854)
                     /gene="guaB"
                     /locus_tag="ACHJ69_003241"
     CDS             complement(8388..9854)
                     /gene="guaB"
                     /locus_tag="ACHJ69_003241"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO5016170.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            10016..11392
                     /gene="xseA"
                     /locus_tag="ACHJ69_003242"
     CDS             10016..11392
                     /gene="xseA"
                     /locus_tag="ACHJ69_003242"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO5016171.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQTEGLFDLQYKKSLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANKRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPIVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     THQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTRQLVQQNPQSRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKVGETLITRLGDGVVISEVSAVTKTRK
                     SRKKTSNP"
     gene            complement(11461..19512)
                     /locus_tag="ACHJ69_003243"
                     /pseudo
     CDS             complement(11461..19512)
                     /locus_tag="ACHJ69_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF823 domain-containing adhesin"
     gene            complement(19633..20610)
                     /locus_tag="ACHJ69_003244"
     CDS             complement(19633..20610)
                     /locus_tag="ACHJ69_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family autotransporter-associated protein"
                     /protein_id="HFO5016172.1"
                     /translation="MKQDKRRGLTRIALALALAGYCVAPVALAEDSAWVDSGETNIFQ
                     GTIPWLYSEGGSATTDADRVTLTSDLKGARPQGSETDKRLYSGDKLTVSWEIGDTEGD
                     VDLGGLGDNAKTIDTIRWMSYKDAQGGDPKELATKVTSYTLTDADRGRYIGIEITPTT
                     QTGTPNVGTALHLYDVSTVSGGGSDSDNVAPGPVVNQNLKVAIFVDGTSINLINGSTP
                     IELGKTYVAKLYSDENKNGKFDAGTDADVTANYDFRWVLSGSSQQLGTSGGIVNSSFD
                     NNNLVIPATNDEARTNLNGPARDGKEALSIPTNGDGVQGYKLHIIYKHK"
     gene            complement(20657..22834)
                     /gene="sinH"
                     /locus_tag="ACHJ69_003245"
     CDS             complement(20657..22834)
                     /gene="sinH"
                     /locus_tag="ACHJ69_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HFO5016173.1"
                     /translation="MLRWKRCIILTFISGAAFAAPEINVKQNESLPDLGSQAAQQDEQ
                     TNKGKSLKERGADYVINSATQGFENLTPEALESQARSYLQSQITSTAQSYIEDTLSPY
                     GKVRSNLSIGQGGDLDGSSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRYN
                     FDKYLLGGNIFYDYDFTRGHRRLGLGAEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEE
                     RPARGWDIRAEAWLPAYPQLGGKIVFEQYYGNEVALFGTDSLEKDPFAVTLGVKYQPV
                     PLIVVGTDFKAGTGDNTDLSVNATLNYQFGVPLKDQLDPDKVSAAHSLMGSRHDFVER
                     NNFIVLEYKEKDPLYVTLWLKADVTNEHPECVIKDTPEEAVGLEKCKWTINALINHHY
                     KIVAASWQAKNNAARTLVMPVIKENTLTEGNNNHWNLVLPAWQYSSDQAEQEKLNTWR
                     VRLALEDEKGNRQNSGVVEITVQQDRKIELIVNNIANVPEENNHSHEASAQADGVDGV
                     VMDLDVTDSFGDNTDRNGDALPEDNLTPQLYDAQDKRVTLTNKPCSTDNPCVFIAKQD
                     KEKGTVTLSSTLPGTYRWKAKAVPYDDSNYVDVTFLGAEIGGLNAFIYRVGAAKPSNL
                     IGKDKEPLPLNNIYRFVLWRDNNKDGVFQQVEKLTDEEMAQYDYKWEFTGKSINGEVG
                     AQANTSNEDIVIPATNREAAQTYGAQAGDGLQGYGLRVLYTKK"
BASE COUNT         5522 a         5912 c         5713 g         5748 t
ORIGIN      
        1 ggggtagcag aggttatatt catcggctct gtcagccatc gagagcagta tgagctttaa
       61 tgacgagcta cctggatctg tctcccaggc ccaatctgtt gcatgtctgc tcatgattaa
      121 tctccgctat cagcttgaat gttgtgggga ggaattaatc atgatctgct taatctctgc
      181 cctgatacga cggtttgatt ccatggtgca ctcaacacag tgtccgttgt aaacccagcg
      241 ttcactgtca tgtccgtgct tacatgtttt tccggtgtag tagcgtttaa gtccgcgctt
      301 tgcggcatca atacgtgtaa tgatttccat ggtaagccct gttattagta ttgggattac
      361 ggttattttg tgctgacaca aaaaaaagat caaccatatt tggtttttta ttacctttaa
      421 ggtgcgaata gatatgaaaa gaccgccgga tggcggtcta cagagggttg tggctggata
      481 tcatgagtag aagaagtatg ccagttctgc ttttgagcgc agccattgtc ttgttttaca
      541 ggctttaaaa agcccattca tcaatacctt acctggcatt ttgcgcttac ctgttaagtg
      601 agtctggata tagtgactcg tcgttccggc ttcctgtgcg aaggcttcac gctcatccgg
      661 agtaagtgca agccagtgct ttttgaaatc gaaatgtccg ttatcgctca tagctattgc
      721 ctgatattta tttcagataa taaatattca cccataaggt aacaaaaatc aaggatagtt
      781 acctatgggg tgcatttacc cgttgggtaa tattgcttta aattgaatca tctactgatt
      841 catatataag gcgattttcc agaaaatgaa aagtatccag gacgtccgca ggcaaaatct
      901 caacgacttg atcgaccgtg aattcaatgg tgttcagacg cggatggcag aaaaacttgg
      961 aactcaggca aatctggtaa accgctgggc tcttggcaag aaggttatcg gcgaccaggt
     1021 tgcgcgaaaa attgaagctg ccgccaataa accccgtaac tggcttgata tcgatcgctc
     1081 gctttctcag gaaggttttc agcctgtcgg cccaagcgac attggacagc tggcggctca
     1141 caacctggaa cgctggatga gcgaaagccg cgacctttca acgcagggaa aactacaccg
     1201 cgcatccggc gtcgcccagg tgacaatcag ccgcctgtta aacaatgagg tcagcgtttc
     1261 catttccacc ctggagaatg ttgcatccgc attcgggcgt cacggctatg aattactgat
     1321 tcacccgcac gaccctgcga ccatcaacta tgatcgctcg cgctacgcat tgttacctga
     1381 aacagagaag gcaaagatcg aaagttacat tgaatttgtc atcaaccaga acgaaaaaaa
     1441 caaacaataa aatcatattt ttcagtaagt aagccgcctt ctggcggctt ttttattgcc
     1501 tgttcgatta cctaacgggt aattttttta actcatatct attgacatca aaccaaatac
     1561 gcataattat tacctcaacg gtaacaaacc gaggtaacaa gttatgcagt ggaaaatcat
     1621 caacggttgg tactgcgtta ctgcatgcgg attcatgagc tggaagttcc gcaccttaca
     1681 ggaaggcatt aagtgggctt tcgtcagcaa agaagctcgc gatgtggcca acgataacga
     1741 gatatgggag ggttgataat gaacgttaat cagcagaaaa atcttcaaaa aatcatgctg
     1801 gcattcgaca aggactaccg cctgtcagaa cagctatatg accgacaagt tgaactgatt
     1861 gagagtatcc ggcttcatca actggcctca actttcgacg ttgtaacagg caaaggcgtt
     1921 cgtcaggaag tactggaggc tgctaaagac agccctgagt tcgaagaact gatggatgcc
     1981 taccggcgag aggcaatggc aattatcgcc cgctgggatc tggctgatca gcttgatggg
     2041 cagagggacg cggcatgaaa ccgggaattt atttcgacat cagcaacgaa gactaccacg
     2101 ccggtgacgg cgtgagtaag tcgcaactgg acatggttgc caagaatccg gcgcttctta
     2161 aatgggttca ggcagcacca gaagacgaag agaaaaagtc tgcactggat atgggaaccg
     2221 cattgcactg tctgcttctg gaacctggag aattcgacaa gcgcttcatc gtttcaccga
     2281 aattcgatcg tcggacaaaa caaggtaaag ctgacgaaga agcatttctt cgtgatgtgg
     2341 cggatatggg tattacggta cttgatgtcg agcagtggcg aaaactggag ctgatgcgtg
     2401 atagcgcaat ggctcacccg gctgcacgct ggatgttgga agcacctggt tactgcgaag
     2461 cgtcaatgta ctggaacgat gaagagactg gtgagttgtg ccgaattcgt ccagacaaat
     2521 ggctgaacga gcacaacgtg atcgtcgacg tgaaaaaggt tgcagatatg gaccgttttg
     2581 cacgtcacat cgaggaattc cgctaccacg tgcaggacgc aatgtaccgc gaaggcgcaa
     2641 tgagggttac tggtcagccg catggttttt tctttcttgc cgtgagcgaa agcattgatt
     2701 gtggtcggta tccggtacgc gtgttcgagc tggatgcgcc ggatgtcgat gccgggcacg
     2761 ctctgttccg ccgggatctg aatacctatc acgaatgccg catcaacgat gaatggggcg
     2821 gagtggaaat tattaaacgc cctgactggg cacgtaaaca ggatatgtat gtatgagcaa
     2881 tgatatcgca atcacatcac aaccaggcgc aactgtaggc actgctgcgg caatcttcag
     2941 ccccgagggc atgaatcaac tggtgcgttt cgcggagttg atgtcacaaa gcaaagcaac
     3001 tgtaccgaaa catcttgaag gcaaacctgc cgattgcctg gcggtgacca tgcaggcggc
     3061 acagtgggga atgaacccgt tcgccgtggc gcagaaaacg catgtggtaa acggaacgtt
     3121 aggctacgaa gcacagttgg taaacgcggt cgtatcctct tccagcctgc tggcgacacg
     3181 cctgaattat cgctggagcg gtgactggtc gaatgttaac ggcaaaacag ataaatcacc
     3241 taatctgacg gtaactgtgt cagcagttct taaaggagaa gcagaaccac gtgagcttac
     3301 catcagtatg gcgcaagccg gagtgcgtaa ctctccattg tgggaacagg atccgcgcca
     3361 gcagcttgcc tatctttgca cgaaacgatg ggctcgcctg cacgctcctg atgtacttct
     3421 cggtgtttac acaccagacg aattacagga aacggcaccg cgcgttgagc gagacattac
     3481 tccgcaaacg accactgctg cgggaatgaa cagtctgatc aacgctaaaa cagtgaaaaa
     3541 gcctgatgag caaacgcgta aagcggatag ccgtgatcca gaagaaatgc tgatggcctt
     3601 taccagcgca gcgatgaatt acagcactgt ctccgaacta gataaggctt acaaatacat
     3661 tgcacaaaaa ctttcagatg atgacgaact gctggcaaaa gccaccgacg tttacagcgt
     3721 tcgccgggaa gaattaaacg aaacatctat gtaaccacca ccgcggcgcc acgcgcgccg
     3781 cactgcaacc aagagaggta tttatgaaag gtgcattagg taagaaggaa ctcctggcgg
     3841 tggtgccact gtcatggagc actatcgacc gtatggagcg cgcaggtgaa tttcctaaac
     3901 gctggtatat cactgacaaa cgctgcgcat ggaaccgtga cgaagttgag cgttggcttg
     3961 atgaacgtca ggcagcaagc ccggcagagt tccagggtaa aaagcctccg gttcagcaac
     4021 gtgtatatcg tcctgtgagc aacgctgcat gagtgtgctg ctaaggcact ggagcaaatg
     4081 gtcaggatgg tacttattcc tggcctctgt ttcagcatgg ctttatctgc tggcattaat
     4141 tttcagagag ggttggatta agtgagaaag ttaagccgac ttgaaaaata tcacatgaac
     4201 aaggtttcaa tgcgcagtcc gtcaaagatt gtcgccgtta ctcctgcggc gatagagatc
     4261 gaaaaacgcg cgattgaaag agagaaaaaa gggcagttcc gcattgccgc tcacctttgg
     4321 cttcagtgta tggatgttgc ttctggtgat gttgagcgtg caaggatcgc ggttcgcagg
     4381 gaccaatgta tcacaaaagg taacggcctt cgccgtggcg actatagcgg cataggatgt
     4441 tgtggggtgg tttatgacta agaaatacac actaatctat gcagatcccc cctgggtata
     4501 ccgggacaaa gccgcagatg gtaatcgcgg tgccggtttt aaatatccgg ttatgagtgt
     4561 gctggatatc tgccgccttc ctgtgtggga tttggccgat gaaaactgtc tgttggccat
     4621 gtggtgggtg ccaacacaac cactagaagc actaaaagtt gttgaagcct ggggattccg
     4681 tctgatgacc atgaagggat tcacgtggat aaaatgtggt agtcgacaac cagataaact
     4741 ggttatgggt atgggacaca tgactcgcgc caatagtgaa gattgcctgt ttgcggtaaa
     4801 gggaaaacta cctacgcgca ttaatgcagg gatcgttcag tcatttaccg caccgcggct
     4861 tgagcattca agaaagccag atatcgttcg tgaaaaactt gtgcaattgt taggcgatgt
     4921 ttctcgcatt gaactgttcg cccgccagtc gtctcatggc ttcgatgttt ggggtaatca
     4981 gtgcgaagac ccggcagtgc aactacaccc tggatacgcg ttggatattg gcggattaac
     5041 aaatgcattc agcaatgctc cgctgtcacc aacagacaac caggggcggg agcgtgcagc
     5101 atgaaccggg catcaccagc agatttaagg aaatgccttg aaactgcaaa catgcttgca
     5161 cacagcggga tcaggtttgt tcccattccc gctgtcactg atgctgaatt tgcaacgctg
     5221 tcagcaatat tcgaaaacaa aattgaatca ctggcagcag aagcaaagat ggaagaaaat
     5281 cagcagaact attaaacgtt attcccccgc catccacttc tcaaacttcg acggggagaa
     5341 cggaatcaga tccgtatgct ccccgttaat ccaggaatca atcatatcgg cccactgctg
     5401 caacatgtag gcgcgctgtc tggcgtattc cgctttgtta tatacggcgc gcacaccttt
     5461 gtgctcatgt gccagagcct tttcaatcca gtctgaagga taaccagcct catgcaacaa
     5521 cgtactggct gtacggcgca tatcgtgtac ggtgaagccc tgaatatgct caccatcttc
     5581 atttattatt ttcaccgttc tgtcgatcag agagttcagc gcggcattag ataatggctt
     5641 ccggaaattg taacgaccag gaaccagata ttcacttcca ccagcgcaca tctgcaaccc
     5701 aaccaatata tcctgtgcct gtttaggcag gtaaataacg tgcgcccggc ttcccttcat
     5761 gcggtctgaa ggaattgtcc atgtccattt tttaaaatct atttcatccc acgttgcatt
     5821 ggtgaattcg cctttacgaa ccatagtgat aagcaccagc tttaaagcca ttttcatagt
     5881 gcccatagca ccaatggcat ccaacgtgcg gaagaacagg ccaatttctt ctggtgtcag
     5941 tgttcgctct cgtggtttaa atatggcgat agacgaaggt ttaatgtcag ccgcaggatt
     6001 aaacaaacca tgaccacggt cattggcgtg acggtatacg ctgctgatga tttccctggc
     6061 ctgtactgct gttgcccgac caccgcgttc gacaatccgg tcacacaaat cacgaaccat
     6121 cgatgtggta atttcagcca tcattttgtt gccaagaacc ggaagtatgt cacggtcgat
     6181 caccgcctgc ttcattgcgc gggtactgtc agccaggatg acgtgtttca tataactgtc
     6241 ggtatgtacc gcaaacgtct cggcaccacg aatctttttg ataccgtcac gtttagccgc
     6301 agccggcgac tggcctgctt taagcagctt ctttgcagca atcagttctt ctcgcgcttc
     6361 tgccaggctg ataccgtcac gcccatactg cccgattacc agtgtttcgc ggcgaccgtt
     6421 gatacggtag tcatagcgaa acgagaccgt gcctgacgta agcacagcta catacagccc
     6481 gtcacgatcg gagaccttgt acagtttgtc ctgcggcttg aggtttttta attttgtatc
     6541 ggtaagcaca attcacccgt atagaaacca ttttcatgac ggtatgagag tataccttta
     6601 aggtaatacc gtcacctgta ccgccgaaaa atatggtgta gagtgaatag aaatgaatac
     6661 ataaaaacaa aaaccctctg caaaaacaga gggttgagtt ggcatctgaa taggaatgat
     6721 ttgctatgag ttagctgtta atcattccca ctcaatggta gctggcggct tgccgctgat
     6781 gtcatacacc acgcgggaaa taccgttcac ttcattgata atacggttgg aaacgcggcc
     6841 gaggaagtcg tacggcagat gtgcccagtg tgcggtcata aagtcgatgg tttcgacagc
     6901 acgcagagag acaacccagt catacttacg accatcgccc attacgccaa cggaacgtac
     6961 tggcaggaag acggtgaacg cctggctgac tttgtcgtac aggtccgctt tacgcagttc
     7021 ttcaatgaag atggcgtcag cacggcgcag caggtcacaa tactctttct tcacttctcc
     7081 cagaacacga acgccaaggc ctggtcccgg gaacgggtga cggtacaaca tgtcatacgg
     7141 taggcccagc tccagaccaa tcttacgcac ttcgtctttg aacagctctt tcagcggttc
     7201 aaccaggccc atcttcatct ctttcggcag gccacccacg ttgtggtgag atttgatgac
     7261 gtgtgcttta ccggttgcag aagcagcaga ttcgataacg tcagggtaga tggtgccctg
     7321 cgccagccac ttcacgtctt ccagtttcag cgcttcttca tcgaatacct caacgaaaac
     7381 gcgaccgatg attttacgtt ttgcttccgg atcgttttcg ccagccagcg ctgacaagaa
     7441 gcgatcttct gccggtacgt gaacaatatt cagaccaaag tgatcgccaa acatatccag
     7501 aacctgctct gcttcgttga gacgcagcag gccgttgtcg acgaatacgc aagtcaggtt
     7561 tttaccgata gcgcggtgca acagcattgc ggtaacggag gaatccacgc caccagagag
     7621 gccgaggatg actttatcgt cgcccacttg ctcgcggatg cgggctacag cgtcgtcgat
     7681 aattttcgct ggcgtccaca gggcttcaca ctggcagata tcacgcacaa aacgctccag
     7741 catgcgcata ccctggcggg tgtgagtcac ttccgggtgg aactgtacgc catagaagcg
     7801 tttttcttcg ttggccatga tggcaaacgg gcagctttcg gtgctggcta cggtgacgaa
     7861 gtcggacggg atagcggtaa ctttatcgcc gtggctcatc cagacgtcca gcagcggttt
     7921 accatctgcg gtcagcgcat cttcgatacc gcgaaccagt gcgctgtcgt ttacgacttc
     7981 aacctgcgcg tagccaaatt cacgttcatt agaagcctca acgtgaccgc ccaactgcat
     8041 cgccatggtc tgcataccat agcaaacgcc gaataccggt acgcctgctt caaagacata
     8101 ctgcggcgca cgcgggctgt tttcttcagt agtactttcc gggccgccgg aaagaataat
     8161 gccgcttgga ttgaagtcac gaatttgtgc ttctgtcaca tcccacgccc acagttcgca
     8221 gtaaacaccc agctcacgca cgcggcgcgc aaccagttga gtgtactgag aaccgaagtc
     8281 cagaatgagg atgcgatgct tatgaatgtt ttccgtcatt gacgcttatt ccgaggcaag
     8341 tgaaacagat aatataaatc gcccgacatg aagtcgggcg aagagaatca ggagcccaga
     8401 cggtagttcg gggactcttt agtaatggtc acgtcgtgaa cgtggctttc ctgaataccc
     8461 gcaccgctga tacgtacaaa ctccgcttta gtacgcagtt cgtcgatagt accacagccg
     8521 gtcaggccca tacaggagcg caggccgccc atctgctggt gaatgatctc tttcaggcga
     8581 cctttatagg ctacgcggcc ttcgatacct tccggcacca gtttgtcggc agcgttgtcg
     8641 ctctggaagt aacggtcaga agaacctttg gacatcgcgc ccagggaacc cataccacgg
     8701 taagatttgt aagaacggcc ctggtagagt tcgatttcac ccggagattc ttcagtacct
     8761 gccagcatgg aacctaccat caccgcgctt gcgccagcgg cgatagcttt agcgatatcg
     8821 cctgagaaac ggataccgcc gtcagcgata accggaatgc cggtaccttc cagggcttct
     8881 actgcatcag caacggcggt gatctgcgga acaccgacac cagtcacgat acgcgtcgta
     8941 cagatagaac cagggccgat acccacttta acggcactgc aaccggcttc tgccagcgcg
     9001 cgggcacctg cagcagttgc cacgttgccg ccgatgattt gcaggtcagg atatttagcg
     9061 cgagtttcac ggatacgctg cagaacacct tcagagtgac cgtgagagga gtcgatcagc
     9121 agaacgtcaa cgcctgcggc aaccagcgca tcgacacgct cttcgttacc agcgccagca
     9181 ccaaccgctg caccaacacg cagacgacct tgttcgtctt tacaggcgtt cggtttacgt
     9241 tctgctttct ggaaatcttt cacggtgatc atgccgatca ggtggaattc atcatcaacc
     9301 accagcgcct tttcaacgcg tttttcgtgc atttttgcca gcaccacttc acgggcttcg
     9361 ccttcacgca cagttaccag acgctctttc ggcgtcatgt aaacgctaac cggttgattc
     9421 agatcggtca caaaacgcac gtcacgaccg gtgatgatgc cgaccagttc gttttcttcg
     9481 gtaacgaccg ggtaacccgc aaagccgtta cgctcggtca gttctttcac ttcacgcagc
     9541 gtcgtggttg gcagtacggt ctgcggatcg gttaccacgc cagattcgtg ttttttcaca
     9601 cggcgaactt cttctgcctg gcgttcaatg gacatgtttt tgtggataaa gccgataccg
     9661 ccttcctgag ccagagcaat agccaggcgc gcttccgtta cggtatccat tgctgcggaa
     9721 agcataggga tattcagacg aatagttttc gtcagctggg tgctgaggtc agcagtattc
     9781 ggcagaacgg tagaatgagc aggaacgagg agaacgtcgt caaacgtcag agcttcttta
     9841 gcgatacgta gcatgggcaa tatctcgacc agagtggtta ataaatattg ccgcggcatt
     9901 atacagagcg taaccgattg catctacccc tttttgcaaa aaatgcttgc tatccccgga
     9961 gggcgggtta ctatcgactg aataacctgc tgatttagaa tttgatctcg ctcacatgtt
    10021 accttctcaa tcccctgcaa tttttaccgt tagccgcctg aatcaaacgg ttcgtctgct
    10081 gcttgagcat gagatggggc aggtgtggat cagcggcgaa atctctaatt ttacacaacc
    10141 agcctccggt cactggtact ttacgcttaa agacgatacc gcgcaggtgc gctgtgcgat
    10201 gttccgcaac agcaaccgcc gggtcacctt ccgcccacag cacgggcaac aggttttagt
    10261 tcgcgccaat attacgctct acgagccgcg cggcgattat caaattatcg tcgaaagtat
    10321 gcagccggcc ggtgaaggat tgctgcaaca gaagtacgaa cagcttaaag cgaagttgca
    10381 gactgaaggt ctgttcgatc tgcaatacaa aaaatcactc ccctcccctg cgcattgcgt
    10441 tggtgtgatc acctcaaaaa ccggtgctgc gctacatgat attttgcatg tgttaaaacg
    10501 tcgcgatcct tctctgccag tgattattta tcccaccgcc gtacaggggg atgatgcgcc
    10561 ggggcaaatc gttcgtgcca ttgaactggc gaataagcgc aacgagtgtg acgtgttgat
    10621 cgttggccgt ggcggtggtt cgctggaaga tttatggagt tttaatgatg agcgtgtggc
    10681 gcgggcgatt tttgccagtc gcattccgat agtcagcgcc gtcgggcatg agacggatgt
    10741 gacgattgcc gactttgttg ctgatctgcg tgcgccaacg ccgtcagccg ccgctgaagt
    10801 ggtgagccga aatcagcaag aattgctacg tcaggtgcaa tcgacccatc aacggctgga
    10861 aatggcgatg gattattatc tcgccaaccg cacgcgtcgt tttacgcaga ttcatcatcg
    10921 cttgcagcaa cagcatccgc agctccggct ggcacgtcag caaactatgc tcgaacgtct
    10981 gcaaaagcgg atgagctttg cgctggagaa tcagcttaaa cgcgcgggtc agcaacagca
    11041 gcgattaaca cggcaactcg tccagcaaaa tccgcagtcg cgtattcatc gcgcgcaaac
    11101 gcgcattcag caactggaat atcgtttagc agaaaccctg cgcgcacagc ttagcgccac
    11161 gcgtgaacgt ttcggtaatg cagtaacaca cctcgaagcc gtaagcccac tgtcaacgct
    11221 ggcgcgcggt tatagcgtca ccagcgccgc tgatggcgcg gtgttaaaac aggttaagca
    11281 agtgaaagtg ggtgagacac tgatcacacg actgggagat ggtgtggtga ttagtgaagt
    11341 cagcgccgta acaaaaaccc gcaaatcacg taaaaaaacg tctaatccgt agaccggata
    11401 agaggcgcca gcatcgcatc cggcaataac tcccaggcta ctccagatcc gccatgctgg
    11461 ttactggaag caggtcacca gacccggttt atttattagc gtgatactac aatcctgtgc
    11521 tccagtacct tcgtccatcc cacactgttg agaggacaaa taaggaaaac tgggtgaaga
    11581 cggccatccc agtgaattaa aaacagtatg caacaactct aattcattgt atgtcggtgc
    11641 attttctatg ctgccacact gccttgcgat atcccattta ctggcatcaa tgatatgtgc
    11701 agaagcccag ttttcgtagt taataatcgg tgaggatacg ggattagccc cggacggcaa
    11761 ttccgtcgtt aacaacggtc gatgtagcga tttactattc accactaccg tgtctgccat
    11821 atgtccccaa aagtgcgccg aggaaacatt tgggctggtt ggtaccgtaa aaatagcgtc
    11881 cagcgtgtct gtcgcggaag cgtaattagc cagagtaacc gtcagtggcg ttttataacc
    11941 aggggtatta tcctggcgga gcgaaaaact tgccgtccca tccgcgcctg tcatgccact
    12001 aatggtatta ccgctttcac tcagcgtaac agccgaacct gataacggct gaacgacgag
    12061 atcgtccatc gccactacat tgccatacaa cgttgcccca gcgttacgcg gcgatgcctc
    12121 accacgtttc aaagtaaatt caacattcgg caccgtattc ccggcgctgt ctttcaccgt
    12181 cacggtaacg gacatggctt cgcctttttt cactttcgcc gcctgagtgg ctgcatcaaa
    12241 cgctgttgag gtaagtgtga cgctggaaac cgttgaacgc gcgttagcca gacaaggctg
    12301 aaatgatttt gttgaacccg tatacagcgt gccatttctc aaatcaaccg actggtcttt
    12361 accatcggcg gatttatcct ctgcccacca ggtgtaatac gcaggccagc cagtttcagt
    12421 gacaaacgtg ttattcggat aacgcgcaga caacgaactt aaatcattga atagtggctg
    12481 ccttgctact tcacaagggc tcacactcgc atctgatttc tgtgttgggg taaagtacgg
    12541 ccagtcttca ttattgttat tgatcttgcc gacgccagaa gtcgatgtca gttcactcca
    12601 caatttcggg cgttcaaata ccgcaccatt cactgccgtc aaggttgctg gcatatgccc
    12661 ccagtagtta gccttcgcag aatccgggct ggtcactacc gtgaatatca ggtcctgatt
    12721 agccgtaacc tgcggatttc gcgccaatga agctgtaaac ggcgttacca gaccaacggt
    12781 gttattttgg gtaacgttga acgttatcgt gccatcactg ccggtgaagc cgctccatgc
    12841 cgacgtattc tggttgaagg agatacgcgc cgatgtcggg gtgacttcat ctaaaatgag
    12901 atcatctacg gtattggagg atacagagat ccctgaacgg cttttcgcct gcacgcgctt
    12961 gagcgtaaca ccttcgccgc caacaggatt accattggca tctttgatcg tcaccacgat
    13021 gggaagcatc tcaccttttt tcacagatga cgtgggggta ccaccattgc tggcagttcg
    13081 gccctcatca taggcagtcg atgacagcgt aaggcttgct ggtgccgaat gtggctgcgt
    13141 cagacagagc attaaagcgt atccattaat agtcgtcgtc gattgtcccg ttttcaaatt
    13201 aatgacgccg taataccacg tcaacgcgtc agtggtgctc atttcataag tccaccaatt
    13261 tccacttccg gcagttatag gcaaccccaa atctgtcagt attgtcccgc ctggatgatc
    13321 attatatagc ccctgtaact cattgagacg cggctgatac tcaaccgggc aatccggcgt
    13381 aactttattg gtataaaaaa ggtatgacca cgactcatta ttattactta ccgaggaccc
    13441 caaggtcgct tctgctgcca gtagcggtcg tttaaaggtc acccccgccc tactggtaaa
    13501 cgtgtcgggc atatgacccc agtattttgc tttcggtgag ttcggactgg ttatcacagt
    13561 gaaaataaca tccatcgtgg cactttttgt ggtgtcgcgc atcaatgttg cagtgagcgg
    13621 agttgccagc ccataagtct cgttttgcgt cacatcaaaa gtcgctttac catcacttcc
    13681 tgtcgtcaga agtgcctgtg ccccattatt atagagtgac atgtttactc cggcaggggt
    13741 gaccggcacg atagtaagat catcatacgt agaatctagc ctgttttttg cacgatccaa
    13801 cgcagtcccg cgtatgagat taaagtttgc gcctggcaac agatttcctc cgctatcccg
    13861 cacaatcacc gtcatccgga tcgccgtacc tttcgccgcc gttgcactag ggcgaccatc
    13921 gttgctggcc gttttctccg catccagtgc cgtggaggtc atctttatag aagctgccac
    13981 tgcatgaggg tttaccagac aggacacaaa tgccacggtg tttgataatg ccctggagct
    14041 tgatccattc gataaattaa tagactgatc gtaccagacg ttcgcgtagg caattttatc
    14101 gtaagcccac caggtgttag tcagcggaag gcctaaatcg gtccctatcg caccattagg
    14161 atgttcactg tataactctt gaaattcgct taataacggt tgataaggtt cgccgcagtc
    14221 tgatttgctg acatcatttt ttgctgccgt attcaaagaa agccatgatt cattattagc
    14281 catggttgac tgccccgctg gatgctcggc agtaagcaat ggccgcttaa aggcaattcc
    14341 cgaccgggta gtaaacgttt caggcatatg cccccagtaa cttgccagtg gcgtatccgg
    14401 gctggttatg acggtaaaca tgaccggtaa tttgttggat tctatcgtca gctgatacaa
    14461 ctgggcgtac atattggtta ccaggcccat agaatcatct tgttttaccg taaaggaggt
    14521 agtcccgtcc gcgccagtca ccccataaag caggcttgaa gaactattca tcctcgcaga
    14581 agcgcctgcc gcgttaatta ccgtaatcga cttatttatc atctcggcat tttgacggtt
    14641 cttcgagcct tcacgtctga gaataaaggg gacattgggc acgctatttc cctgcgcatt
    14701 tttagtcacc acgcgaatca ccgcgtcttc gccttttttc actttcaacg cactcaaggt
    14761 gtcgtcaact ttgacaaatt gcaccgtatc attcgcctca acaatgatct gactggcaac
    14821 ggcgacaggc gtggtccggc agctcagtaa tgcaccacca gatgagggca tctgcgtttc
    14881 atctcctgtc ttcagcgaaa cgacatcata cgtaaaggtt tgagtgaacg tatctgcaac
    14941 ggtattcgag atatagaacc tcgacactgg ccagccaata gcatcatgta atgcgctact
    15001 cccctggtgc gcataaatgg ccctaagttc attgtcagtt ggaacatact ccgcgccaca
    15061 accatttatc gaggtatcgt tagcctggcc cgacgggaac gtagaccagg cctccccctc
    15121 ttccaccagg gtaccgatac tggtggtagc actattctcg tccctcagta aaggccttcg
    15181 ataaataaga ccgtcatcgc ctgtcgccgt ttcagccata tgcccccaga atttggcttt
    15241 tggcgaatcc gggctggtaa gcaccgtaaa gacaacggga agcgtcgatt tagtgctgct
    15301 ggaggaatcc aacgctgccg tgagttctgt ttttagcccg gtagtctggt cttgcttcaa
    15361 tgtgaaagtg gtgatgccat cagcccccgt cacgccataa ataaccaggg ttgttgaaag
    15421 aaaatcatct tgagtatttc cccaggcatc cgttaccgcc agcgaagcaa tcgacgtcgt
    15481 agttgacaca ttggccctgt tcacgctggt gtttcgtttg agggtaaatg cggtatttcc
    15541 cacagggtta ccctgcgcat ctttagtggt cactcgtaat acggcttctt cgcctttttt
    15601 cgctttgaca acattattcg tactgtcaaa ttttgtgggg tctgcagctt caagaacaat
    15661 ttggcttgcc ttaacaatcg gcgtcgtctg gcaggtaagc agttcttttg tggcagatgg
    15721 aattatcgaa ctggtgcctg aactgagatt gacggccttg aagtcacgct gttccaggct
    15781 actactacct gtcgtgctgg aaaggtaact tgaggcgacg ggccagccct gaaccgtttt
    15841 catcgcattg ccgcggttgg cttcatacaa tgactccagc ccggcctggg agggaacata
    15901 ttcactacca caaccattag aggatgtgct tatagcctgg gtttgggtaa attgcgccca
    15961 gtcttcattc tcttctgcaa tcgctgttcg acccgacgta ctggataact ctttcaacaa
    16021 taatgggcgt ttaaagaccg ttccatctgc agccgtcact gtttcaggca tatgccccca
    16081 cattttcgcc ttatcgctgt ccggactggt tacaacggta aaaatggtat cgagggtcgc
    16141 ttttttcgtt gtatctgaat agagcgtagc ggtcagcgac gttttcgtgc catgggtatc
    16201 cgggcgggta atagtgagaa ttttagttcc gtcgctgccg gttatggcgc tgtatgctgc
    16261 agcggtatta ttaagcgaag tttcgtccgc caggccacca ttgaccacaa ccggcgcaac
    16321 cagcgcatca ccgctaccag caacgtgttt ttcctctgag cgggtataac catcgccacg
    16381 tttcagggta aagggtatat cacccaatgg attgccctgc gcatctttta ccgtcacctt
    16441 tacctgtagc gtttcgcctt ttttcagctt cgccgcattt gcagccgcgt tccactgcgc
    16501 cggatctacc acttccagcg taatgctgct ggcaggattg ttagccgtcg tcaggcaact
    16561 gacataaacc gccgtgctac cgccaacggt ccgcgcccca tcgttgaggg ctatggtgta
    16621 ataatgcggt acctgatctg cgggcgaact gctccagtag ggctgacgct gcgttggcca
    16681 gccgtgtact gtctgtacac cgttaccgtt attggcatca tagagcgcgc taagctgaga
    16741 acggcgtggg agcatattgg cgccacagcc acctttactg gtgctggagg cgttggcctg
    16801 gctgactcgc gaccagactt cgttattttc cgttaatgtg ccgtccggac tgaacacttc
    16861 cgcagccagt cgggggcggg caaaggttaa agcatcgacg gtaatcgtct catccatatg
    16921 cccccacatc tgcgcgccaa ccgcatccgg actggtgagc gtagtgaaaa tcacattgta
    16981 attaacagta ttgggaatat aggagttcac aggcacaata tttagcggcg tcgataagcc
    17041 cacaccttgc gattgcttaa tttccacagt cgcaaaaccc tacgcatccg tagtaccggc
    17101 atagactaaa gacggttgcg atgttccgta gagtgtgcca ttcatctcga ttgcgccgcg
    17161 ggttggatca tcaaaacctg tagtctgccc ctgacgattt ttccccatat ctttagtgat
    17221 ggtgaacgcg gtatacggca cgggtgcatt attcaaggca ttaaaggtgc gtaccgtcat
    17281 ggtgatcgtg tcacccactt tggcctgcgc tctgctgcct gcggaaacat cacgatcggt
    17341 ggtcgcggca atcttggcga ccatctcatt gcccgaacaa gagagatagt tttgcttaaa
    17401 gccggaatag gtgtcgatgt tgcccgtggc caggtttacc gacgcgtgcg acgtctcttc
    17461 caccgcacta agatacgctt tttgcaacgt tggccagcca tattcggtgc cgatggcatt
    17521 ggctggatga gcggcaaaca aactgtgcaa tgaactttgg ctcggaatat gacctaaacc
    17581 acactcagcc tgcatactgc tattctgatc aaacagcgcc cagtcttcgt tattttcccg
    17641 caccgatccc agttcgttgt cggtttcatc cgccagccgt gggcgtttaa agatattgtt
    17701 cgcttcaata atgcccaaca tatggcccca catccgcgct ttgtcggtat cggggctggt
    17761 gatcaccgta aaaataacgt ctttctcatc cgaggcagta aaatcactgc gcattctcgc
    17821 ggtgatgtga gttttcaccc ccacgccgcc tggctgggtc agcgtcagag tggcctctcc
    17881 gttagcatca gtgatccctt cataggtatg ggcgtcaact ttgcgaacgt tactgccgcc
    17941 atcaatttgt accggatgcg tctcccacgc ggcgtcagtt tgattcttac ggttaatgcc
    18001 gtcatccaga tgcagcgaaa agtagtagta agccagcggc tgatcgttgt tcttgctatc
    18061 ggtaatcgtc agacgtaaag aagcctcttc ccccaccttt actttcatcg cttttatcgt
    18121 cgaatcatag ctgcccggcg tcagtaccag cttcggttca acatcgggcg cttctttatc
    18181 gacacagctc acaataaacg tcgtatcgtc cggtttttgc gcttcgctgc ggttcgaaac
    18241 atccgccgca tggtgctggc cgctcatccc cgccagtgat gaccagtaga aattaccctg
    18301 catcggccaa ccagccacat cctgcactga cgttggcacc accgtcgcca gcgcaccgaa
    18361 ctgacgcatt ccgggcaaaa tgtcgcaatg tttatccgcg ccgctccagg taaaggtgga
    18421 ccaggtttca ttgtgatcgt taaccgtacc gccttcatta tcgacttccg ccgccagttt
    18481 tggtcgacta aaagtgtaat cacgggcctt cagcgtatcg ggcatatgac cccacattgt
    18541 agcctgcgga acatccgggc tggtgaggac ggtaaagatc actgccgttt cactggcctg
    18601 agcaataccc gcgaggctcg cgaccaacgg cgtttttaca ccagggccgt tggcctgggt
    18661 gaccgtaacc gtggcaacac cattcgcatc ggtagtacca cgatattccg tcgcggtggt
    18721 ggttaattcg gtcgtgccca gttttacagg cgcagtattg ttcaccacac cctggcggtt
    18781 ttcagcatct ttgcgtttga taacaaaagg aatattcccc acaacattac cgacgcaatc
    18841 tttggtggtg accgttaacg tgatactgcc cccaaccgtc attttcgccg cacctgagga
    18901 ttcgtcatag cccgcagcgc tgacggaaat aaggtcaccc tgcgcgggcg caatcttcag
    18961 cgtcactttc tgatcggcga gcgtcagaac actttcatag ggatagcccg tcgaggtgag
    19021 taaatataac agcggcatgg tctgctgggg atcaggctgc gcccaggcga ccagcgaacg
    19081 cccggcaagt ccctgtgcac aaaacgagcg atctgcgact ggctgtgggt ccagtaaggt
    19141 gtccggcgtg gcggcatccg cccaaactaa agatacagcc ggtggcgttt ggttttccca
    19201 acgtggggtg gcaatgatat cgccttctct gtcttgcggg ttggtgacga gcaatttaga
    19261 gacgctaaca tccgcgttga attcactcgg tttagcggta aacgctacct catgcgtctt
    19321 tgtaggatcc agaaagacac tgccctggta gacggatatg ctggcgctgt cggcggtggg
    19381 cacggtaccg ttaaattcgc cgaccgtatc tgaagagtct tgccatgcag attctgccca
    19441 tgaccaccca ggcagcattg cgcttatcag caatgcgcat aacacaagga ttttattcag
    19501 ggtcagggac atgacagaaa ctccaccggt tgttaaacgg acgcttgtcg catcaatata
    19561 ttttgcttaa tccgggatta agcaaaatat gcggaaacag cattcccttg cgggaatgct
    19621 gctgagtacg cgttatttgt gtttgtaaat aatgtgaagt ttgtaaccct gtaccccgtc
    19681 gccgttggtc gggatggaaa gtgcctcttt tccatcgcgc gcagggccgt taaggttggt
    19741 tctggcttcg tcgttggtcg cagggatgac caaattgtta ttatcgaagc ttgagttaac
    19801 gatgccaccc gaagtgccaa gctgttggct gctgccagaa agtacccaac ggaagtcata
    19861 attggcggtg acgtcagcat cggtacccgc atcaaacttg ccatttttgt tctcatccga
    19921 gtacagtttg gccacgtagg ttttgccaag ttcgattggt gtgctaccgt tgataaggtt
    19981 gatactggta ccatcaacaa agatggcgac tttcaggttc tggttaacca ccggccccgg
    20041 tgcaacgtta tcgctgtcgc ttccgccgcc gctgacagta gaaacgtcat aaagatgcag
    20101 cgcagtcccg acgtttggcg taccggtctg cgtggttggc gtaatttcaa taccgatata
    20161 gcgaccacgg tcggcatcgg taagagtgta actggtcacc ttcgttgcca gctcttttgg
    20221 atcccccccc tgcgcatcct tgtagctcat ccagcggata gtatcgatag ttttcgcgtt
    20281 atcgcccaat cctccaagat ccacgtcgcc ttcggtatca ccaatttccc aacttacggt
    20341 taatttatca cccgagtaaa gacgcttgtc cgtttcactg ccttgcgggc gagcgccttt
    20401 tagatcagaa gtcaacgtta cacggtcggc atctgtcgta gcacttcccc cttccgaata
    20461 gagccacgga atggtcccct ggaaaatatt ggtttcaccg ctgtcgaccc aggcgctgtc
    20521 ttcagccagc gccacaggtg ccacacaata acctgccagt gccagcgcta atgcgatccg
    20581 ggtcagaccg cgtcttttat cctgcttcat tgatgctctc cttaatgaac cttcagcgac
    20641 tggccttacg ccaatcttat tttttggtat acagcacgcg taaaccgtac ccctgcaagc
    20701 catctcccgc ttgtgcgcca taggtttgcg ccgcttcacg gttagtggcc ggaatcacaa
    20761 tgtcctcatt ggaagtgttc gcctgtgcac ccacttcacc attgatgctc ttgccggtaa
    20821 attcccactt gtagtcgtac tgcgccatct cttcgtcagt cagtttttcg acctgctgga
    20881 agacaccgtc tttattgttg tcgcgccata aaacaaatcg ataaatgttg ttaagcggca
    20941 acggttcttt atctttacct atcaggttgc tgggtttagc cgcccccact cgatagataa
    21001 aagcatttag cccaccaatt tctgccccga ggaaagtgac atccacatag ttactgtcat
    21061 cgtagggcac ggcttttgct ttccagcgat aggtgccggg taaggtactg gagagggtga
    21121 cagtgccctt ttctttatct tgtttagcga taaaaacgca ggggttgtcg gtcgagcaag
    21181 gcttgttggt taacgtcact cgtttgtcct gcgcgtcgta aagctgcggc gtgaggttat
    21241 cttccggcag cgcatcgcca ttgcggtcgg tgttatcgcc aaagctgtcg gttacatcga
    21301 gatccatcac cacaccatca acgccatctg cctgagcgct ggcttcgtgg ctgtggttgt
    21361 tctcttctgg cacgttcgcg atgttattaa caatcaactc tattttacgg tcctgctgaa
    21421 cggtgatttc caccacgcca gagttctgtc ggttgccctt ttcatcttcc agcgccagac
    21481 gtactcgcca ggtattgagt ttttcttgtt cggcttgatc ggaactgtac tgccaggcag
    21541 gcagcaccag gttccagtgg ttattgttac cctctgtcag agtattctct ttgataaccg
    21601 gcatcaccag cgtgcgggca gcattgtttt tcgcctgcca agaggcagca acgattttgt
    21661 aatgatgatt aatgagtgcg ttaatggtcc acttacattt ttccagaccg acggcttctt
    21721 cgggcgtatc tttaatcacg cactcgggat gttcattcgt gacgtcggct ttcagccaca
    21781 aagtgacata gaggggatct ttctctttgt attccagaac aataaagttg ttacgttcaa
    21841 caaagtcatg acggctaccc atgagcgagt gagccgcgct cactttgtca ggatcaagct
    21901 ggtctttgag cgggacgcca aactggtagt tcagcgttgc gttaacagaa agatcggtat
    21961 tatcgccagt gccagctttg aaatctgtac cgacaacaat gagcggaacg ggctggtatt
    22021 tcacacccag cgtgacagcg aagggatctt tctccagact atcggtacca aaaagcgcga
    22081 cttcattccc ataatattgt tcgaagacaa tttttccgcc aagctgcggg taagcgggta
    22141 gccaggcttc agcgcgaata tcccagccgc gcgcagggcg ttcttcataa aagtcgaaat
    22201 cttcagagtc tttccagtca gaaagtgggt gataatagtt gcctgagaat tttaaataat
    22261 ccgtccaggc ttcggcgcct aaacctaaac ggcgatgtcc acgggtaaag tcataatcat
    22321 aaaatatatt tccacccaat agatatttat caaaattata ccttacccca aggccaatat
    22381 tcccgatcgt acgatcttct tttcgttgcg cagaaaattg gctgaaataa acagtggttt
    22441 gattatcgta ccagggaaca aaataatcga tggaactgcc atccagatcg ccgccctgac
    22501 caatggagag gttcgaacgg accttaccgt agggagagag tgtgtcttca atataagatt
    22561 gtgcggttga ggtgatttga ctttgcagat agcttctggc ctgagattcc agcgcctcag
    22621 gggtcaagtt ttcaaaccct tgcgtggcgg agttgatgac gtaatcggct ccgcgctctt
    22681 tcagcgattt acccttgttg gtttgttcat cctgttgtgc tgcctggcta cctaaatcag
    22741 gtaacgattc gttttgctta acatttatct ctggcgccgc gaaagcagca ccagagataa
    22801 atgttagaat aatacagcgt ttccatcgca gcatgcactt tccctgtgac aatgatacag
    22861 tcaaaaataa gctttaatat tgtatccctg attat
//
LOCUS       DAZFFE010000079        22690 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFFE010000079 DAZFFE010000000
VERSION     DAZFFE010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.079"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..351
                     /locus_tag="ACHJ69_003246"
     CDS             <1..351
                     /locus_tag="ACHJ69_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO5016174.1"
                     /translation="KVEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKA
                     GILPEGFFWTDAENNDVPMTAGELMALSEAAEKAMFTKGMEIHVRQRTMKKELEKLTS
                     ADEILAYRVGWAQE"
     gene            complement(564..776)
                     /locus_tag="ACHJ69_003247"
                     /pseudo
     CDS             complement(564..776)
                     /locus_tag="ACHJ69_003247"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(916..1003)
                     /locus_tag="ACHJ69_003248"
     tRNA            complement(916..1003)
                     /locus_tag="ACHJ69_003248"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(967..969),aa:Ser,seq:tga)
     gene            1430..2548
                     /gene="hyaA"
                     /locus_tag="ACHJ69_003249"
     CDS             1430..2548
                     /gene="hyaA"
                     /locus_tag="ACHJ69_003249"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO5016175.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            2545..4338
                     /gene="hyaB"
                     /locus_tag="ACHJ69_003250"
     CDS             2545..4338
                     /gene="hyaB"
                     /locus_tag="ACHJ69_003250"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO5016176.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTVDFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALKLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            4357..5064
                     /gene="hyaC"
                     /locus_tag="ACHJ69_003251"
     CDS             4357..5064
                     /gene="hyaC"
                     /locus_tag="ACHJ69_003251"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO5016177.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            5061..5648
                     /gene="hyaD"
                     /locus_tag="ACHJ69_003252"
     CDS             5061..5648
                     /gene="hyaD"
                     /locus_tag="ACHJ69_003252"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO5016178.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEDVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAYIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMRLEEQG"
     gene            5645..6043
                     /gene="hyaE"
                     /locus_tag="ACHJ69_003253"
     CDS             5645..6043
                     /gene="hyaE"
                     /locus_tag="ACHJ69_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO5016179.1"
                     /translation="MSNDTPFNALWQRMLARGWTPVSECRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            6040..6897
                     /gene="hyaF"
                     /locus_tag="ACHJ69_003254"
     CDS             6040..6897
                     /gene="hyaF"
                     /locus_tag="ACHJ69_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO5016180.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCRVNGEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRHGEQLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAYELLAHVRNPDAQPHSINMTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            7031..8575
                     /gene="appC"
                     /locus_tag="ACHJ69_003255"
     CDS             7031..8575
                     /gene="appC"
                     /locus_tag="ACHJ69_003255"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO5016181.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQHPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLVIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            8587..9723
                     /gene="appB"
                     /locus_tag="ACHJ69_003256"
     CDS             8587..9723
                     /gene="appB"
                     /locus_tag="ACHJ69_003256"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO5016182.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            9736..9828
                     /gene="cbdX"
                     /locus_tag="ACHJ69_003257"
     CDS             9736..9828
                     /gene="cbdX"
                     /locus_tag="ACHJ69_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO5016183.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            9908..11218
                     /gene="appA"
                     /locus_tag="ACHJ69_003258"
     CDS             9908..11218
                     /gene="appA"
                     /locus_tag="ACHJ69_003258"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO5016184.1"
                     /translation="MKAILIPFLSLLIPLTPKSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQP
                     GQVAVIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGAIADFAGHRQTAFRELERVLNFPQSNLCLNRKKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPSQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACALHQDK"
     gene            complement(11206..11418)
                     /gene="cspH"
                     /locus_tag="ACHJ69_003259"
     CDS             complement(11206..11418)
                     /gene="cspH"
                     /locus_tag="ACHJ69_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO5016185.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            11704..11916
                     /gene="cspG"
                     /locus_tag="ACHJ69_003260"
     CDS             11704..11916
                     /gene="cspG"
                     /locus_tag="ACHJ69_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO5016186.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            11927..12115
                     /gene="ymcF"
                     /locus_tag="ACHJ69_003261"
     CDS             11927..12115
                     /gene="ymcF"
                     /locus_tag="ACHJ69_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO5016187.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            12090..12320
                     /gene="ymcE"
                     /locus_tag="ACHJ69_003262"
     CDS             12090..12320
                     /gene="ymcE"
                     /locus_tag="ACHJ69_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO5016188.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLIRVPGTNTLLACCCLLRPEEVNSEY"
     gene            12310..12483
                     /gene="gnsA"
                     /locus_tag="ACHJ69_003263"
     CDS             12310..12483
                     /gene="gnsA"
                     /locus_tag="ACHJ69_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO5016189.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDIKIKIM"
     gene            complement(12532..13605)
                     /gene="yccM"
                     /locus_tag="ACHJ69_003264"
     CDS             complement(12532..13605)
                     /gene="yccM"
                     /locus_tag="ACHJ69_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO5016190.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKTTQLLLLAINI
                     YIAITFWYWVRYYETAGSTTFITRPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISMIVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKSCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVMGIMFAAIGYAMYAG
                     VWQSPVPEELYQRLIPQSPMIGH"
     gene            complement(13677..16420)
                     /gene="torS"
                     /locus_tag="ACHJ69_003265"
                     /pseudo
     CDS             complement(13677..16420)
                     /gene="torS"
                     /locus_tag="ACHJ69_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            16503..17531
                     /gene="torT"
                     /locus_tag="ACHJ69_003266"
     CDS             16503..17531
                     /gene="torT"
                     /locus_tag="ACHJ69_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO5016191.1"
                     /translation="MRVLLLLLLSLFMLSAFSADNLLRWHDAQHFSVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPHVKSRVGVPWFQMGYQPGRYLVQ
                     WSHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVMTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(17504..18196)
                     /gene="torR"
                     /locus_tag="ACHJ69_003267"
     CDS             complement(17504..18196)
                     /gene="torR"
                     /locus_tag="ACHJ69_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO5016192.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            18326..19498
                     /gene="torC"
                     /locus_tag="ACHJ69_003268"
     CDS             18326..19498
                     /gene="torC"
                     /locus_tag="ACHJ69_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO5016193.1"
                     /translation="MRKLWNALRRPSARWSVLALVATGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            19498..22044
                     /gene="torA"
                     /locus_tag="ACHJ69_003269"
     CDS             19498..22044
                     /gene="torA"
                     /locus_tag="ACHJ69_003269"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO5016194.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFISPQDASARGIRHGDV
                     VRVFNVRGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            22041..22640
                     /gene="torD"
                     /locus_tag="ACHJ69_003270"
     CDS             22041..22640
                     /gene="torD"
                     /locus_tag="ACHJ69_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO5016195.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNGLENSIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDGLRQKTLTA
                     LREWLPEFAARCRQYDRFGFYAALSQLLLVLVECDYQKR"
BASE COUNT         5327 a         5753 c         6208 g         5402 t
ORIGIN      
        1 aaggtggaaa ttaatgcctg gcgtaatgcg atggaagcgg cgaactacac gtttgagcac
       61 aatgggcgta aatgggacta cgggaagtca acgcagacgc gtcttgagcc gtcggtggct
      121 gccgcgaaag caggaatact gccggagggt tttttctgga cggatgcgga gaataacgac
      181 gtcccgatga cggctgggga gctgatggca ctcagtgagg cggcggagaa ggcgatgttc
      241 accaaaggga tggaaattca tgtcagacag cgcaccatga agaaagagct ggaaaagctg
      301 accagtgcgg atgagatact ggcatacagg gttggctggg cacaggaata acagacaaaa
      361 agatggggga ccatcacctc ccccatgaac tgcgttgatt atgtaattat tgtgaagaga
      421 ccttatctac acagataaag agtgccacgg aaaagttaat cctgaaagtg ataccggtct
      481 catttccaga gcatcctgtt gatatggatg atttttcaga tttccggaag tccgtatttt
      541 gcccgtgcgc ggttacatgc ttcattcgcg atgccatttt cgccggacat tgcgaattcc
      601 ctgcatgtgg acggacggtt tttgtaaaca gagcaatatg cgttttcacc cggagttccc
      661 gccagagcga ctgaccggaa cctttccgcc agcatcgtca gtttctgccc agtaaaaaga
      721 gacgcggaaa atgcacaaca ggcaccacac gtcatgcatg gattggggtt attcataatt
      781 catcggtata gcgataaatc ataaagagaa aaatctatct ggtatgtgtt gagctaaata
      841 taccaattag tataccaagt gtatactaag agcaaaaaaa gaaaggggct accatttggt
      901 aaccccttga ttgtttggcg gaagcgcaga gattcgaact ctggaaccct ttcgggtcgc
      961 cggttttcaa gaccggtgcc ttcaaccgct cggccacact tccggaatga cgcgcactat
     1021 aaacatcccg atgcggcgtg taaaccccta atttgtttgt ttgcctgaaa aacagccaaa
     1081 agtgcattaa tagcgtgaaa taacagcaga ttgatcattt catcaccatg aattccttct
     1141 cttttactcg tttagcaacc ggctaaacat ccccaccgcc cggtaaaaag aaaaataggt
     1201 ccatttttat cgctaaaaga taaatccaca cagtttgtat tgttttgtgc aaaagtttca
     1261 ctacgcttta ttaacaatac tttctggcga cgtgcgccag tgcagaagga tgagctttcg
     1321 ttttcagcat ctcacgtgaa gcgatggttt gccttgctac agggacgtcg cttgccgacc
     1381 atgtgcgccc ggtgtcctgc cggtgtcgca aggagaagag acgtgcgata tgaataatga
     1441 ggaaacattt taccaggcca tgcggcgtca gggcgttacc cggcgcagct ttctcaaata
     1501 ttgtagtctg gctgccacgt cgctgggatt aggcgcggga atggcaccaa agattgcctg
     1561 ggcgctggag aataaaccgc gcattccggt ggtgtggatc cacggtctgg aatgcacctg
     1621 ctgtaccgaa tcttttatcc gctccgctca cccactggcg aaggacgtca tcctttccct
     1681 gatttccctc gattacgacg atactttgat ggctgccgcc ggaacccagg cggaagaagt
     1741 ctttgaagac atcatcacgc aatacaatgg caaatatatc ctcgccgttg aaggtaatcc
     1801 gccgctgggc gagcagggga tgttctgtat cagcagcggt cgaccgttta ttgagaaact
     1861 caaacgtgcc gctgccggag ccagtgcgat tatcgcctgg ggaacctgcg cgtcctgggg
     1921 ctgcgtgcag gccgcgcgac ccaatccgac gcaggcaacg cctatcgaca aagtcatcac
     1981 cgacaaacct attatcaaag tacctggctg cccgccgatc ccggatgtga tgagcgccat
     2041 cattacttac atggtgacct ttgatcgctt gccagatgtc gacagaatgg gccgtccgtt
     2101 gatgttctat ggtcagcgaa tccacgataa atgctatcgc cgcgctcact tcgacgccgg
     2161 agagttcgtg cagagttggg atgatgacgc tgcccgcaaa ggttactgcc tgtacaaaat
     2221 gggctgcaaa gggcctacca cctataacgc ctgttcctcc acacgctgga atgatggcgt
     2281 ttctttccca atccagtctg gtcacggctg cctgggttgt gcggaaaatg gtttctggga
     2341 tcgcggttcg ttctacagcc gcgtggtcga tattccacaa atgggtaccc attccaccgc
     2401 cgataccgtc ggtttaaccg cacttggcgt ggtggcagcg gctgttggtg tgcacgcagt
     2461 cgccagcgcc gttgaccagc gcagacgtca taaccagcaa cctacagaaa ccgaacatca
     2521 gccaggcaat gaggataaac aggcatgagc actcagtacg aaactcaggg atacaccatc
     2581 aacaatgccg gacgccgcct ggtggtcgac ccgattacgc gcattgaagg ccacatgcgc
     2641 tgcgaagtga atattaacga tcagaatgtg atcaccaatg ccgtctcctg cggcaccatg
     2701 tttcgcgggc tggagatcat tctgcaaggg cgtgacccgc gcgatgcgtg ggcgttcgtt
     2761 gaacgtatct gcggcgtctg tactggcgta cacgccctgg cttcggttta cgccatcgaa
     2821 gatgccatcg gtattaaagt gccggacaac gccaatatca tccgcaacat tatgctggca
     2881 acgctctggt gccacgatca tctggtgcac ttctatcagc ttgccgggat ggactggatc
     2941 gatgtgttag atgcgctgaa agccgacccg cggaaaacct ccgaactggc gcaaagcctc
     3001 tcctcatggc cgaaatcatc ccctggctat ttcttcgacg tacaaaaccg cctgaagaaa
     3061 ttcgttgaag gcgggcagtt ggggatcttc cgcaatggct actgggggca cccgcagtac
     3121 aaactgccgc cagaagccaa cctgatgggc tttgcccact atctcgaagc tctcgatttc
     3181 cagcgtgaaa ttgtcaaaat ccacgcggtc tttggtggta aaaacccgca tccaaactgg
     3241 attgtcggcg ggatgccttg cgccattaac attgacgaaa gcggcgcagt cggggcggtc
     3301 aatatggaac gcctgaacct ggtgcagtcg atcattaccc gtacggtgga cttcattaac
     3361 aacgtgatga tccccgacgc cttagccatc ggccagttca acaaaccgtg gagcgaaatc
     3421 ggcacgggtc tttctgataa atgtgtcctc agctacggcg cattcccgga tattgccaac
     3481 gactttggcg agaaaagtct gctgatgcct ggcggcgcgg tgattaacgg cgacttcaac
     3541 aatgtgctgc cagtggattt ggttgatccg cagcaggtgc aggagtttgt cgaccacgcc
     3601 tggtatcgtt atcccaacga tcaggtcggg cgtcatccgt tcgatggcat caccgatccg
     3661 tggtacaacc ccggcgatgt caaaggcagc gataccaaca ttcagcagct gaatgaacag
     3721 gaacgctact cgtggatcaa agcgccacgc tggcgcggta acgcgatgga agtggggccg
     3781 ctggcacgca cgttaatcgc ttatcacaaa ggcgatgctg cgaccgttga gtcggtcgat
     3841 cgtatgatgt cggcgttgaa actgccgctt tccggcatcc agtcaacgtt gggccgcatt
     3901 ttgtgtcgcg cgcacgaagc gcagtgggcc gcaggtaagt tgcagtattt cttcgataaa
     3961 ctgatgacca acctgaaaaa cggcaatctt gccaccgctt ccacggaaaa atgggaacca
     4021 gcaacctggc cgacagagtg ccgtggtgtc ggttttaccg aagcgccgcg cggggcgtta
     4081 ggccactggg ctgccattcg cgatggcaag attgatctct accagtgcgt ggtgccgacc
     4141 acctggaacg ccagcccgcg cgatcccaaa gggcagattg gcgcttatga agcggcgctg
     4201 atgaacacca aaatggcgat ccccgagcaa ccgctggaga tcctgcgtac tctgcacagc
     4261 tttgacccgt gcctcgcctg ttcaacacac gtgctgggcg acgacggtag cgagcttatc
     4321 tccgtgcagg tgcgttaaca gcgaaggaga atcatcatgc aacagaaaag cgacaacgtt
     4381 gtcagtcact atgtctttga agcacccgtg cggatatggc actggctgac agtgttatgc
     4441 atggcggtgt tgatggtcac cggttacttt atcggcaagc cgctaccttc cgtcagcggc
     4501 gaggcgacgt atctgttcta tatgggctac atcaggttaa ttcacttcag cgccgggatg
     4561 atttttaccg tggtcttgct gatgcggatc tactgggctt ttgttggcaa tcgttactcc
     4621 cgcgagctgt ttatcgtgcc ggtatggcgt aaaagctggt ggcagggcgt gtggtatgaa
     4681 atccgctggt atctgtttct ggcaaaacgt ccgagtgccg atataggcca taaccccatc
     4741 gcccaggcgg cgatgttcgg ctatttcctg atgtcagttt ttatgatcat caccggcttt
     4801 gcgctgtaca gcgaacacag ccagtacgct atttttgcgc cgttccgtta tgtggtggaa
     4861 tttttctact ggacgggtgg caactcaatg gacattcaca gctggcatcg gctggggatg
     4921 tggctgattg gcgcgtttgt gatcggtcat gtctacatgg cgctgcgtga agacatcatg
     4981 tccgacgaca cggtgatctc cactatggtc aacggctacc gtagccacaa atttggcaaa
     5041 ataagtaaca aggagcgttc atgagcgagc aacgcgtggt ggtaatgggg ctgggcaacc
     5101 tgctgtgggc cgatgaaggc ttcggcgtgc gggtggcgga acggctgtat gcccattacc
     5161 actggccaga ggatgtggag attgtcgatg gcggcaccca aggactgaac ttgctggggt
     5221 atgtcgaaag cgccagtcat ctgttgattc tcgatgccat tgattacggg ctggaacccg
     5281 gaacgctacg aacgtatgcc ggagaacgca ttccggctta tctcagcgcg aagaaaatga
     5341 gcctgcacca gaacagtttc tccgaagtgc tggcgctggc agatatccgc ggacatctgc
     5401 cagcatatat tgccctcgtc ggtctgcaac ccgcgatgct cgacgactac ggcggcagcc
     5461 tgagcgaact ggcgcgggag caactgcccg ctgcggaaca ggcggcgctg gcgcagctgg
     5521 ctgcgtgggg aattgtgccg caaccggcta atgaatcgcg ctgtctcaat tatgactgtc
     5581 tgtcgatgga aaattacgaa ggcgttcgct tgcgtcagta caggatgagg ctggaggaac
     5641 agggatgagc aacgacacac catttaatgc gttgtggcaa cgaatgctgg cacgtggctg
     5701 gacgccggtc agtgaatgcc gtcttgacga ctggcttacg caagcgccag acggcgtggt
     5761 gttattaagc agtgacccga aacgcacgcc agaagtcagc gataacccgg taatgattgg
     5821 cgaattactg cgcgaatttc ccgactatac atggcaggta gcgattgctg atcttgagca
     5881 gagcgaagcc atcggcgatc gctttggcgt ctttcgcttt cccgccacat tagtgtttac
     5941 cggcggaaac tatcgcggcg tgctgaatgg tattcacccg tgggcggaac tgataaacct
     6001 gatgcgcggg cttgtcgaac cgcaacagga gcgtgcttca tgagcgaaac ttttttccat
     6061 ctgctggggc caggaacgca accgaacgat gacagtttca gcatgaatcc actgccgatc
     6121 acctgtcggg tgaatggtga accgagtatg gcggccctgg agcaatgtgc tcacagcccg
     6181 caggtgattg cgctgttaaa cgagttacaa catcaactaa gcgaacgcca gccgccgctg
     6241 ggtgaggtac tggcagtcga tctgttaaat ctcaacgccg acgatcgtca ctttatcaat
     6301 acgcttctcg gggaagggga agtgtcagtg cgcattcagc aggctgacga cagtgaaagt
     6361 gaaattcagg aggcgatctt ctgcggatta tggcgggtac gcagacgtca cggcgaacag
     6421 ttgctggaag acaaactgga ggctggctgc gcaccgctgg cgttgtggca ggcggcaacg
     6481 caaaacgtct tgccgacaga ttcgctgtta ccgccgccca ttgatggcct gatgaatggc
     6541 ctaccgttgg cctatgaatt actggcacat gtacgtaacc ccgacgcgca gccgcacagc
     6601 attaatatga cgcaattacc catcagcgaa gctgaccggc tttttctctc acgtctctgt
     6661 gggccgggaa atattcagat tcgtaccatt ggctatggcg agagctatat caacgccacg
     6721 gggttacgcc atgtctggca tttacgctgt acggacacct taaaaggccc gttactggaa
     6781 agttatgaaa tctgcccaat accggaagtg gtgctggcag cgccagaaga tttggtcgac
     6841 tctgcgcagc ggcttagcga ggtatgccag tggctggcgg agggggcacc gacgtaaaaa
     6901 gacggtaagt atcgctttca ggcttatgaa tatcgcaatc ggcgaatacc tctggtcgta
     6961 gagtttcagg ataaagaggg agatctacca ttaacgggtt atttttctct cttcgcctac
     7021 aggagtgcgc atgtgggatg tcattgattt atcgcgctgg cagtttgctc tgaccgcgct
     7081 gtatcacttt ttatttgtac cccttaccct ggggctgatt tttttgctgg ctatcatgga
     7141 aaccatttac gtggtcaccg gcaaaacaat ctaccgcgat atgacgcgct tctggggtaa
     7201 gctcttcggt atcaactttg ctcttggcgt ggctaccggc ctgaccatgg agtttcagtt
     7261 tggtactaac tggtcattct attccaacta tgtgggcgat atttttggcg caccgctggc
     7321 gatggaagca ttaatggcct tcttcctcga atccaccttt gtcgggctgt tcttcttcgg
     7381 ctggcagcgg ctgaataaat accagcacct gctggtgacg tggctggtgg cgttcggttc
     7441 aaatctctct gcgttgtgga tattgaatgc caacggttgg atgcaacacc cgactggtgc
     7501 gcattttgat atcgacaccc tgcgtatgga gatgaccagt ttcagcgagc tggtctttaa
     7561 cccggtcagc caggtgaaat ttgtgcacac cgtaatggcg ggatacgtga ccggggctat
     7621 gtttattatg gcgatcagcg cctggtattt actgcgcgga cgggagcgcg atgtcgcatt
     7681 acgctcgttt gccatcggtt ccgtcttcgg tactctggtg attatcggta cgctgcaact
     7741 cggagacagt tctgcgtatg aagtcgcgca agtacaaccg gtaaaactgg cggcgatgga
     7801 aggggagtgg caaacggaac ctgcacctgc accgttccat gtggttgcct ggccggaaca
     7861 ggatcaagag cgtaacgcct ttgccatcaa aattcccgcg ctgctaggaa tcctcgccac
     7921 tcattcatta gataaacccg tgccgggtct gaagaatttg atggctgaaa cctacccacg
     7981 cttgcaacgc ggacgtatgg cgtggctgtt aatgcaggaa atatcgcaag gcaatcgtga
     8041 gccgcatgtg ttgcaggcat tccgggagct ggaaggcgac ctgggttacg gcatgttgct
     8101 ctcccgctat gcgccggata tgaatcatgt cacagccgca cagtaccagg cggcgatgcg
     8161 tggcgcgata cctcaggttg cgccggtatt ctggagtttc cgcatcatgg tgggctgtgg
     8221 ttccctgctg ttactggtga tgctgattgc gcttgtccag acgctgcgtg gcaaaatcga
     8281 ccagcatcgc tgggtgctga aaatggcgct ctggagtttg ccactgccgt ggattgcgat
     8341 tgaagccgga tggtttatga ccgagtttgg tcgtcagccg tgggcgatac aggacatctt
     8401 accgacatac tccgcgcact ccgccttaac cacaggacaa ctggctttct cactgatcat
     8461 gatcgtaggg ctttacaccc tgttcttaat cgccgaagtc tacctgatgc agaaatatgc
     8521 ccgtctgggg ccgagcgcga tgcagagtga acaaccgacg cagcaacagg ggtaaaggag
     8581 aaaatcatgt ttgattatga aacattgcgc ttcatctggt ggctgctgat tggcgtgatc
     8641 ctggtggtct ttatgatctc cgacggattt gacatgggga tcggctgtct gctgccgctg
     8701 gtggcgcgta atgatgatga acgtcggata gtgataaaca gcgttggcgc gcactgggaa
     8761 ggcaatcagg tctggttgat cctcgcaggt ggggcattat ttgccgcctg gcccagagtg
     8821 tatgcagcgg cgttttccgg cttttatgtg gcgatgattc tggtgctgtg ctcgctgttc
     8881 ttccgcccgc tggcctttga ttatcgcgga aaaatcgccg atgcacgctg gcgtaaaatg
     8941 tgggatgccg gactggtcat cggcagtctg gtgccgccgg tagtcttcgg tattgcattc
     9001 ggcaacttgc tgctcggtgt gccgtttgcc ttcacaccgc aattacgcgt ggaatatctc
     9061 ggcagcttct ggcaactgct gacgccattc cctttattgt gtggattgct cagtcttggg
     9121 atggtgattt tgcaaggtgg cgtctggtta caactgaaaa ctgttggtgt gattcatctg
     9181 cgttcacagc tggcgaccaa acgcgccgca ctactggtga tgctgtgctt tttgctggcg
     9241 ggttactggc tgtgggttgg tattgatggc tttgtactgc tcgcccagga tgccaacggt
     9301 ccttccaatc cgttaatgaa actggtggca gtgctacctg gtgcctggat gaataatttc
     9361 gtcgaatcgc ccgttttgtg gatcttcccg ctgctggggt tcttctgccc attgctgacg
     9421 gtgatggcga tttatcgtgg tcgcccgggt tggggatttt tgatggcgtc attgatgcaa
     9481 tttggcgtga ttttcacggc aggcatcacg ctgttcccct ttgtcatgcc gtcaagcgtg
     9541 agtccgatct ccagcctgac gttgtgggac agcacttcca gtcagctgac gctgagcatt
     9601 atgttggtaa tcgtgctgat atttttgccc attgtgttgc tctacactct ctggagctac
     9661 tacaaaatgt gggggcgcat gacgacagaa actctccgcc gtaacgaaaa cgagctgtac
     9721 taaggagcag gaacaatgtg gtatttactt tggttcgtcg gcattttgtt gatgtgttcg
     9781 ctctccaccc ttgtgttggt atggctggac ccgcgtctga aaagttaacg aaggtaagcc
     9841 tgatgaggcg cattagcgtc gcatcaggca atcaataatg tcggatatga aaagcggaaa
     9901 catatcgatg aaagcgatct tgatcccatt cttatctctt ctgattccgt taacaccgaa
     9961 atctgcattc gctcagagtg agccggagct gaagctggaa agtgtggtga ttgtcagtcg
    10021 tcacggtgtg cgtgcaccaa ccaaggccac acaactgatg caggatgtca cccctgacgc
    10081 atggccaacc tggccggtaa aactgggttg gctgacaccg cgcggtggtg agttaatcgc
    10141 ttatctcgga cattaccaac gccagcgtct ggtggccgac ggattgctgg caaaaaaggg
    10201 ctgcccgcag ccaggtcagg tcgcggttat tgctgatgtc gacgaacgta cccgtaaaac
    10261 aggcgaagcc ttcgccgccg ggctggcacc tgactgtgca ataaccgtac atacccaggc
    10321 agatacgtcc agtcccgatc cgttatttaa tcctctaaaa actggcgttt gccaactgga
    10381 taacgcgaac gtgactgacg cgatcctcag tagggcagga ggggcaattg ctgactttgc
    10441 cgggcatcgg caaacggcgt ttcgcgaact ggaacgggtg cttaatttcc cgcaatcaaa
    10501 cttgtgcctt aaccgtaaga aacaggacga aagctgttca ttaacgcagg cattaccatc
    10561 ggaactcaag gtgagcgccg acaatgtctc attaaccggt gcggtaagcc tcgcatcaat
    10621 gctgacggag atatttctcc tgcaacaagc acagggaatg ccggagccgg ggtggggaag
    10681 gatcaccgat tcacaccagt ggaacacctt gctaagttta cataacgcgc aattttattt
    10741 actacaacgc acgccagagg ttgcccgcag tcgcgccacc ccgttattgg atttgatcat
    10801 ggcagcgttg acgccccatc catcgcaaaa acaggcgtat ggtgtgacat tacccacttc
    10861 agtgctgttt attgccggac acgatactaa tctggcaaat ctcggcggcg cactggagct
    10921 caactggaca cttccaggtc agccggataa cacgccgcca ggtggtgaac tggtgtttga
    10981 acgctggcgt cggctcagcg ataacagcca gtggattcag gtttcgctgg tcttccagac
    11041 tttacagcag atgcgtgata aaacgccgct gtcattaaat acgccgcccg gagaggtgaa
    11101 actgaccctg gcaggatgtg aagagcgaaa tgcgcagggc atgtgttcgt tggccggttt
    11161 tacgcaaatc gtgaatgaag cacgcatacc agcgtgcgct ctccatcaag acaaataaac
    11221 atttgccgct gttggtccac gcaggccatt tacgcggcaa aattccacgc gtaatcctgg
    11281 tataagcact tctgcgtcgc ggggagtgaa tgcggaaata tggacctgga cttctttacg
    11341 accgtcggag gggataatga atcctttgcc gcttttgcga tcaaaggttt tgacaattcc
    11401 tgtcatttta cgggacaaac aaattcctta ctgaaaatac tgcgctgcac tatacggggt
    11461 taataaaata aagccagcga tatttaagac cgccggacgg ctaaaataaa atttgcttaa
    11521 tctcaattat catgcgttaa tagctgcgtc ggtttgaaag acagacagca tacaaagtag
    11581 tttactaaag cagttctcat tatcaggcat tatccccttc ttttgagtct ctctcctgaa
    11641 cactaagtag tttctgtatt aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa
    11701 aatatgtcta ataaaatgac tggtttagta aaatggttta acgcagataa aggttttggc
    11761 tttatcactc ctgatgatgg cagcaaagac gttttcgtcc atttcactgc catccagagc
    11821 aatgaattcc gcacgctgaa cgaaaatcag aaagttgaat tttctattga gcaggggcaa
    11881 cgtggtcccg cggcagcgaa cgttgttacg ctctaaggtt gccgttatta ctcaacatct
    11941 ccatttccgc tgtccatgtt gccatggttc acagtaccgc acatcggcat tcgatgtgac
    12001 ggagcgaaat cctttgggtg caaagtgcat tttttgtaaa tcgacgatga tcacctttga
    12061 taacgtcgcg ttgcaaatac gcactgacca tgcgccgctg gatttcacaa aataatatca
    12121 ggctccctcg tggagccttt tttatatctg ccttattttt cttcaacgct gtatgtatag
    12181 taagcgataa cctgttgatt attgaatctt tcggggagat ggcttataac atttcttacc
    12241 tgatcagggt accgggaacc aacaccttac tggcgtgttg ctgtctttta agaccagaag
    12301 aggttaacag tgaatattga agagttaaaa aaacaagccg aaacggaaat cgccgacttt
    12361 atcgcgcaaa aaatcgccga gctgaacaag aatacaggga aagaagtctc tgaaattcgc
    12421 ttcaccgcac gagaaaaaat gaccgggctt gaaagttatg atatcaaaat caaaataatg
    12481 tgattttgtg aacatcaccc cgtgcgaggt gatgttccgc ttgttgctaa tttagtgacc
    12541 aatcattggc gattgcggaa ttaagcgttg gtacaattcc tccggcaccg ggctttgcca
    12601 tactcccgca tacattgcgt aaccaatcgc cgcaaacata atccccataa ccagtagcgt
    12661 cattaaccag ccagacaacg caaaggcttt tttatttgcc gcaggttttt gcagtgaaaa
    12721 ggtcaatgtt gaggctaccg gacatgactc tacgcaagtc atacagccgg tacattccac
    12781 tgttcgtacc tgaattaatt tatcgaccgg gatccgtgat gggcaacttt ttgcgcattt
    12841 gccacaatcg atacaacttt cggcattgcg acgaatctta aacggcgata atagcgaaac
    12901 cacgcccatc agcgcgccat atgggcaaag ataacgacac caggcatggc gaataaacag
    12961 gctggcaatc agcaaaacga tcatgctgat taatgtcgcg gtccccatat gacgaaagaa
    13021 atcgagcatt ttaacgtcca tcaccacgct gtagggcgac aacataaaat agtgaatcgc
    13081 ctgagcgggc atcaataacg cgatatagag aaaaaaactc aacagcaaat acttcacgcc
    13141 gcgcagagga atatccagcc agcggggaag gacaaattgc cgaccaaaca gtttgttacc
    13201 gagatcgccg attaattccg aaagcgtacc aaccgggcat aaccatgaac aaaaggcctt
    13261 tttgagtaat agactgatga cgataaaagc gaccaataac agcatcgcgg cggcgtggac
    13321 ggacggtaac tgacctgttg caaggctata tttcagattc atcagaccgg caatcggtag
    13381 ccagccttcg atacctcccg gtctggtgat aaatgtcgta ctacctgccg tttcgtaata
    13441 gcgcacccaa taccagaacg tgatggcaat ataaatatta attgccaaca gtaataattg
    13501 cgtcgtttta cgccaggtcg tggcattgcg ccagtcattc cacggtaatt tgccgcccgt
    13561 cgtgcctggc cgccgctgcc agcgggttct ttttttctct gccatgattt tgccagtccg
    13621 ttaagttgca taccaaatgc cactattcta gttgttctta actggctgat attgattcaa
    13681 atcgcgttca ggtttttctt attaaccatg cttccagagc ggcaacactg cgtgtaattt
    13741 cttcgtgtgg aaggggggca gataatggct gctgctccag ttgtgcgcat tgctggctgg
    13801 cgctacgcat tcccagactc gagcaactgc tttttagctg atgtgcggca cgctttattt
    13861 tctcgttatc ctgactggcg cgggcaatgt cgatttcatc gagaagcggc agggcatgtt
    13921 gtttaaataa tatcagccat tcgtggatct tctccgcccc cattaaatga gcatcttcat
    13981 tgagttgcga tacatccagc ggttgatcgt tattgacttg cagttggaga tagtgcgcca
    14041 gtaattgacc gagcacttca cgcggcaccg gtttagggat aatcccgcga aatagcgaac
    14101 tggtacgctg tcgcagtgtt tcgtcaatga catgggcgct aaagccaatc aaaaccagcg
    14161 acggatattg ctgtgccagt tgtcgggcaa gcgtaatgcc gtcgatatcc ggcagatcaa
    14221 aatccaccag tgcgacagca aacggttcac tattttgcag tgtctctaaa gcctgcgcgg
    14281 catttcctac ggcaacgacc tgcgccccac tgctgttcaa catctcaacg gtaatccgct
    14341 gggttagcgg gttatcttca attaacagca aacgtaaacc gtccagacgc accgcttgat
    14401 tgactgtttt tggcacgggt gcagtggcaa tacgtaacgg caagcgtaaa caaaaacagc
    14461 ttccaacctc cggcgtgctg gtggcgctca gttcgccacc catcgcctgg gccagacggc
    14521 tactgatagt cagccccagc ccggtgccgc cgcgtttgcc gctaacctga acaaatggct
    14581 ggaagatttc agccagtttc gcgggatcaa taccgcagcc gctgtcttcc acttcgacca
    14641 gccattgttc gccatcagtg cgactacgca gaataatgtg cccttcgtcg gtgaaacgca
    14701 gggcgttgct caacaggttg gtaatgacct gacgaatacg ccgcggatcg cccattaacg
    14761 cagtcggtac atcgtcggca atttctgttg ccaggcgaat cgggcgacct ttcacccttc
    14821 cgctcattaa ttgcagggta ctttccagca gcgggcgcgg ttcaaagggt tcatcgctga
    14881 ccgaaacatt cttgccgcct gcttcgatag ccgaataatc gagaatatcg ttgaggatgg
    14941 tcagcaacga ttcaccagag tcggtaatcg cccgcaaatc atcgcgctgg gcgttaagtg
    15001 cggggttatc tgccagcagt tgcgcggtgc cgagaatacc gtacagcggt gtgcggatct
    15061 catggctcat cgccgccaga aacgccgatt ttgcctggct ggctttttct gcttccgccc
    15121 gtgcctgtcg gtgttctatc accagttcct gcaattcagc tgtacgcgct ttgacctgcg
    15181 ccgccagctg ttcgcggtgg cgattcagtg catgaacact gctgcgaaac gcatccatca
    15241 gccgcccgat ggtatccagt tcccgtacgc cagcggtttc cgggaaaggg gagtcgatat
    15301 caccatccag caagcgttgc agcgcctgcg tttgttctgc aagagggcgc gtgactgagc
    15361 gataaaccac gcgccagagg atcagaatca gtgcgcaaag agaaactaat cccagcaata
    15421 acaggctgta ttgcccgcgt gcactggctt tttccagatg tgccagcccg tgctgattac
    15481 gcagctcaat ggtgtcgacc agctgactga cttcgctact aaactgcgcg aattgggcga
    15541 tgttattttg ggcgagagtt tgcaggtgat tgctgatttc actgtcctgc tgatacagcg
    15601 ccagcaaatc gctatattgg ctaacggtag ttaacgttgt tgctacctga gtacgaacac
    15661 ccggatcttc aatgcgtatt tgccgacgtt gcagaatttt caccgcatta ttgagctgct
    15721 tttccagcgt tggcgcattt ttctggatct gttccagccc cagattcatc accatttgct
    15781 gcacccgcag ggcgctaagg cgcagttcat tcatctggtt aacatactca agatcgatat
    15841 caatcagccg atcgagtgca ctttctgcgg cctgacgctg atgttgttcg atcaaatcgt
    15901 aaatcccggc ctgggtcgct ccggcggagg ttgccgcatt attcgcctga ccttgcgcca
    15961 ggcgtgcgat ttcatcggcg gcggcgacta tctgctgact gagttgctgt tgttgctggc
    16021 gaagttgcaa acgctgcccc accagttccc cttgctgacg taacgaacgg gagatctcct
    16081 gctcctgttg ttcaatagcg gtggtgtcaa aaccttgttc ccgtaacgct tgcagcaacg
    16141 cattaatctt caagctttgt gcggtgagca ttcgcccctg cgcctgccac atcttttcgt
    16201 tatcggcact ggtcaggttc tgcgcggcga aaagttccca ggcgctggct tcgctcaact
    16261 ggcgcgccat attcatggta ggaatcaatg cctgagtgtt gtctttttcc acctggctga
    16321 taaagcgcag gttgtaccat cccaccaggg tactggtcag ggttaacagc gccatcaggg
    16381 caaagcccat ccagagtctt cgggtcaggg ttaaattcac ggtcggtgca ctttgggtga
    16441 aaaaggttga gtcgcaaagc ggaatgcatc tagcataaag ccttattatt gatgaggcta
    16501 tcatgcgcgt actgctattg ttactacttt cccttttcat gttatcggca ttttcggctg
    16561 ataacctgtt gcgctggcat gatgcgcagc atttctcggt gcaagcctct acgccgctaa
    16621 aagccaaacg cgcatggaaa ctgtgcgcgc tttatcccag cctgaaagat tcatactggt
    16681 tatcgttgaa ctatggtatg caggaggctg ctcgccgcta cggtgtggat ttaaaagtgc
    16741 tggaggcagg cggctacagc cagttggcta cccagcaagc acaaatcgac cagtgtaaac
    16801 agtggggcgc agaggccatt ttgctcggta gtagcacgac gtcatttccc gacctgcaaa
    16861 agcaggtagc aagtctgccg gtaatcgaac tggtgaatgc tattgatgct ccccacgtga
    16921 aaagccgcgt tggtgtgccc tggtttcaga tgggctatca accaggacga tatctggtgc
    16981 aatggagcca cggtaaacca ctgaacgtgc tgttgatgcc cggacccgat aacgccgggg
    17041 gcagtaagga gatggtcgag ggttttcgcg cagccattgc cggaagcccg gtacgtattg
    17101 ttgatattgc gctcggtgat aacgatattg aaatccagcg taacctgttg caggagatgc
    17161 tggaacgcca tccagaaatc gacgtcgttg ccggaacggc cattgcggca gaggcggcaa
    17221 tgggggaagg gcgcaacctg aaaacgccgc ttaccgtggt gtcgttttat ctttcacatc
    17281 aggtgtatcg cgggttgaag cggggaagag tgattatggc agccagcgat caaatggtct
    17341 ggcaggggga actggcggtt gagcaggcta tcaggcaatt acaggggcaa tcggtgtctg
    17401 ataatgtcag cccaccgatt ttagtcatga cgccgaaaaa tgccgaccgc gaacatatcc
    17461 gccgctcgct gtcaccaggg ggatttcgtc cggtttattt ttatcagcac acatcagcgg
    17521 ctaagaaata accttcgcca tgttgtgtca ccagtaaatc cgcactgagt ttatggcgta
    17581 aacgacgaat taacacatcg acggtgcgca ggtcagggtt ttccacccga cgcgcagaga
    17641 gcatacgcag cagacgttca cggctgagaa tttcgcccgg attagtcaca aatgccacca
    17701 gcatttcata ctctgcgcgg gtcagtttaa tcggctcgcc atcccgctcc agcgtatggc
    17761 gcgacacatt caggcaataa ccggcaaagc gatagcagtt gtcctgagtg tgcggttgcg
    17821 cctgtcgcgc gaggtcgatt cgccagagca aatttttcac tcgtaccacc agttcgcgca
    17881 gctccagcgg tttggtgacg taatcgtctg cgcccatttc cagcccaaca atacggtcaa
    17941 tccgatcgct gcgtccggta accagaataa tccccaccgt tgagcgttct cgcagggcgc
    18001 gggttaacat caggccattt tcatcgggca aattgatatc cagcagaatc aaatccaccg
    18061 gctgattctg cataatttcc cgcagcccgg caccgctcgc cgtaacggaa acggtatacc
    18121 cctcctgagt gaagtaagat tgtaatcgcg cctgggtaac cggctcatct tcaacaataa
    18181 caatgtgatg tggcatcaga gggttttact cattctgttc atatctgttc atattctgcc
    18241 gtaagccgtt catcctgacc agtgccgctg ttcatatttg ctcattaaga tcgcttcact
    18301 aaaccataat tttacagggg ctattatgcg gaaactctgg aacgcgctac gccgacccag
    18361 tgctcgttgg tcggtactgg cgctggtcgc tactgggatt gtgattggca ttgcgctgat
    18421 tgtattgcca cacgttggga tcaaagtcac cagcacaacc gaattttgtg tcagttgcca
    18481 cagtatgcaa ccggtgtatg aagaatataa acagtcggtg catttccaga acgcctccgg
    18541 cgtgcgagct gaatgccatg actgtcatat cccgccggat attccaggca tggtgaagcg
    18601 caaactggaa gcgagcaacg acatttacca gacctttatt gcccactcca ttgatacacc
    18661 tgagaaattt gaagccaaac gcgcggaact tgccgagcgt gaatgggcgc gaatgaaaga
    18721 aaacaactcg gcaacctgcc gctcctgcca taactacgat gcgatggatc atgcgaagca
    18781 gcatcctgaa gcggcgcgtc agatgaaggt ggcagcgaaa gataatcaat cctgcatcga
    18841 ctgtcataaa ggtattgccc accagttacc ggatatgagt agtggcttcc gtaagcagtt
    18901 cgatgagctg cgcgccagtg ctaatgatag tggtgacacg ctgtactcca ttgatattaa
    18961 gccgatttat gcggcgaaag gcgataaaga agcgtctggt tctctgctgc ctgcttcgga
    19021 agtgaaagtc cttaaacgtg acggcgactg gctgcaaatt gaaatcactg gctggacgga
    19081 aagtgcagga cgtcagcgtg tactcaccca attcccaggt aaacgcatct ttgttgcctc
    19141 gattcgtggt gatgtgcagc agcaggtgaa aacactggag aaaaccaccg ttgccgacac
    19201 caataccgag tggagcaagt tgcaggccac tgcgtggatg aagaaaggcg acatggtgaa
    19261 cgatatcaaa ccgatctggg cttatgcgga ttcgctgtac aacggcacct gtaaccagtg
    19321 ccacggcgca ccggaaatat cccactttga cgctaacggt tggatcggca cgctcaacgg
    19381 catgattggc tttaccagcc tcgataaacg tgaagaacgc accttgttga aatatctgca
    19441 aatgaatgcg tctgacaccg caggtaaagc tcacggcgat aagaaggaag aaaaataatg
    19501 aacaataacg atctctttca ggcatcacgt cggcgttttc tggcacaact cggcggctta
    19561 accgtcgccg ggatgctggg gccgtcattg ttaacgccgc gccgtgcgac tgcggcgcaa
    19621 gcggcgactg aggctgtcat ctcgaaagag ggcattctta ccgggtcgca ctggggggct
    19681 atccgcgcga cggtgaagga tggtcgcttt gtggcagcaa aaccgttcga actggataaa
    19741 tatccgtcga aaatgatcgc cggattgccg gatcatgtac acaacgcggc gcgtattcgt
    19801 tacccgatgg tacgcgtgga ctggctgcgt aagcgccatc tgagcgacac ctcccagcgc
    19861 ggtgataacc gttttgtgcg cgtgagctgg gatgaagccc tcgacatgtt ctatgaagaa
    19921 ctggaacgcg tacagaaaac tcacgggccg agtgccttgc tgaccgccag tggttggcaa
    19981 tcgacgggga tgttccataa cgcttcgggg atgctggcga aagctattgc cttgcatggt
    20041 aatagcgttg gtacgggcgg agattactct accggtgctg cgcaggtgat cttgccgcgc
    20101 gtggttggct caatggaagt gtatgaacag caaacctcct ggccgctggt attgcagaac
    20161 agcaaaacca ttgtgctgtg gggctccgat ttgctgaaaa accagcaagc gaactggtgg
    20221 tgcccggatc acgatgttta tgaatattac gagcagttga aagcgaaagt cgccgccggt
    20281 gaaattgagg tcatcagcat cgatccggtt gttacatcca cccatgagta tctggggcgc
    20341 gagcatgtga agcacattgc ggttaacccg caaactgacg tgccgctgca actggcgctg
    20401 gcgtatacgc tgtacagtga aaacctgtac gacaaaaact tcctcgctaa ctactgtgta
    20461 ggttttgagc agttcctgcc gtatctgctg ggtgagaaag acggtcagcc gaaagatgcc
    20521 gcatgggcag aaaaactgac cggcattgat gccgaaacca ttcgtgggct ggcacggcag
    20581 atggcggcga acagaacgca gattattgct ggctggtgcg tacagcgtat gcagcacggt
    20641 gaacagtggg cgtggatgat tgtcgttctg gcggcgatgc tggggcaaat tggcctgcca
    20701 ggtggtggtt ttggttttgg ctggcactat aacggcgcag gcacgccggg gcgtaaaggc
    20761 gttattttga gtggtttctc cggctctacg tcgattccgc ctgttcacga caacagtgac
    20821 tacaaaggct acagcagcac cattccgatt gcccgtttta tcgatgcgat cctcgaaccg
    20881 gggaaagtga tcaactggaa cggtaaatcg gtaaaactgc cgccgctgaa aatgtgtatt
    20941 tttgccggaa ctaacccgtt ccatcgccat cagcagatca accgcattat tgaaggctgg
    21001 cgcaagctgg aaacggttat cgccatagat aaccagtgga cctcaacctg ccgctttgcc
    21061 gatatcgtgc tgcctgcgac cacgcagttt gagcgtaacg atctcgacca gtacggcaac
    21121 cactccaatc gtggcatcat cgccatgaaa caggtcgtgc cgccgcagtt cgaggcgcgc
    21181 aacgactttg atattttccg cgagctgtgc cgccgcttta atcgcgaaga agcctttacc
    21241 gaagggctgg acgagatggg ctggctgaaa cgcatctggc aggaaggtgt acaacagggc
    21301 aaaggacgcg gcgttcattt gccagcgttt gatgacttct ggaataacaa agagtacgtc
    21361 gagtttgacc atccgcagat gtttgttcgc caccaggcat tccgcgaaga tccggatctc
    21421 gaaccgctgg gcacgccgag tggcctgatt gagatctact caaaaaccat tgccgacatg
    21481 aactacgacg attgtcaggg gcatccgatg tggtttgaga aaatcgaacg ctcccacggt
    21541 gggcctggct cgcagaagta tccgttgcat ctgcaatctg tgcatccgga tttccgactt
    21601 cactcgcagt tatgtgagtc ggaaactctg cgtcagcaat ataccgtagc gggtaaagag
    21661 ccagtattta ttagcccgca ggatgccagc gcgcgcggta ttcgtcacgg tgatgtggta
    21721 cgcgtcttta acgttcgtgg tcaggtgttg gctggagcag tggtttctga ccgttatgca
    21781 cccggcgtgg cgcgaattca cgaaggggca tggtacgatc cggacaaagg cggcgagcca
    21841 ggggcgctgt gcaaatacgg taaccccaac gtgttgacca tcgacatcgg tacttcgcag
    21901 ctggcgcagg cgaccagtgc gcacactacg ctggtggaaa ttgagaagta caacggaaca
    21961 gtggagcagg tgacggcgtt taacggcccc gtggagatgg tggcgcagtg cgaatatgtt
    22021 cccgcgtcgc aggtgaaatc atgaccacgc tgacagcaca acagattgcc tgtgtttacg
    22081 cctggctggc gcagttgttc tcccgtgaac tggacgatga acaactgacg caaatcgcca
    22141 gtgcgcagat ggctgaatgg ttttcgttgc tgaaaagcga acctccgctc actgcggcgg
    22201 tgaatgggct ggaaaacagt attgccactc tgacagtacg tgacgatgcc cgtctggaac
    22261 tggccgctga cttttgcggc ctgtttctga tgaccgacaa acaagcggcg ctgccgtatg
    22321 catcggccta caaacaggac gagcaagaga ttaaacgctt gttagttgag gcagggatgg
    22381 agaccagcgg caatttcaac gaaccggcag atcatctggc gatctatctc gagttgttga
    22441 gtcatctgca tttttcgctg ggagagggga ccgttcctgc gcgaagaatc gacggtttgc
    22501 ggcaaaaaac actgacggct ctgcgggaat ggttaccaga gtttgctgcg cgttgtcgac
    22561 agtatgaccg ctttggtttt tacgcggcac taagccagtt attgctggtg ttagtggagt
    22621 gtgactacca aaaaagataa tgccgtttgt aggcctgata agacgcggca ggcgtcgcat
    22681 caggcattag
//
LOCUS       DAZFFE010000080        22553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFFE010000080 DAZFFE010000000
VERSION     DAZFFE010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.080"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            161..1366
                     /gene="gltS"
                     /locus_tag="ACHJ69_003271"
     CDS             161..1366
                     /gene="gltS"
                     /locus_tag="ACHJ69_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO5016196.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLILKKSMGWEVNFDMSLRDPLMLAFFATIGLNANLASLRAGGRVVGVFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFTG"
     gene            complement(1400..3481)
                     /gene="recG"
                     /locus_tag="ACHJ69_003272"
     CDS             complement(1400..3481)
                     /gene="recG"
                     /locus_tag="ACHJ69_003272"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO5016197.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSELETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSTNDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAVAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRIDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMTSFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(3487..4176)
                     /gene="trmH"
                     /locus_tag="ACHJ69_003273"
     CDS             complement(3487..4176)
                     /gene="trmH"
                     /locus_tag="ACHJ69_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO5016198.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWSTMQAAG"
     gene            complement(4183..6291)
                     /gene="spoT"
                     /locus_tag="ACHJ69_003274"
     CDS             complement(4183..6291)
                     /gene="spoT"
                     /locus_tag="ACHJ69_003274"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO5016199.1"
                     /translation="MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(6310..6585)
                     /gene="rpoZ"
                     /locus_tag="ACHJ69_003275"
     CDS             complement(6310..6585)
                     /gene="rpoZ"
                     /locus_tag="ACHJ69_003275"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO5016200.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(6640..7263)
                     /gene="gmk"
                     /locus_tag="ACHJ69_003276"
     CDS             complement(6640..7263)
                     /gene="gmk"
                     /locus_tag="ACHJ69_003276"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO5016201.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            7521..9203
                     /gene="ligB"
                     /locus_tag="ACHJ69_003277"
     CDS             7521..9203
                     /gene="ligB"
                     /locus_tag="ACHJ69_003277"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO5016202.1"
                     /translation="MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGNETRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRL
                     ISAVPQTVSGPLANSTLQGEIFLQRKGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPHLMTDRLKDLATAGFTLTQTYTRAVKNADEVAHVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLV
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARQQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(9200..9817)
                     /gene="yicG"
                     /locus_tag="ACHJ69_003278"
     CDS             complement(9200..9817)
                     /gene="yicG"
                     /locus_tag="ACHJ69_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO5016203.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(10069..10932)
                     /gene="yicC"
                     /locus_tag="ACHJ69_003279"
     CDS             complement(10069..10932)
                     /gene="yicC"
                     /locus_tag="ACHJ69_003279"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO5016204.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIIARETEGQALKVLIEQRL
                     EGVTAEVAKVRAHMPEILQWQRERLIAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            11059..11775
                     /gene="rph"
                     /locus_tag="ACHJ69_003280"
     CDS             11059..11775
                     /gene="rph"
                     /locus_tag="ACHJ69_003280"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO5016205.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            11841..12482
                     /gene="pyrE"
                     /locus_tag="ACHJ69_003281"
     CDS             11841..12482
                     /gene="pyrE"
                     /locus_tag="ACHJ69_003281"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO5016206.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(12519..13115)
                     /gene="slmA"
                     /locus_tag="ACHJ69_003282"
     CDS             complement(12519..13115)
                     /gene="slmA"
                     /locus_tag="ACHJ69_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO5016207.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(13222..13680)
                     /gene="dut"
                     /locus_tag="ACHJ69_003283"
     CDS             complement(13222..13680)
                     /gene="dut"
                     /locus_tag="ACHJ69_003283"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO5016208.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(13658..14878)
                     /gene="coaBC"
                     /locus_tag="ACHJ69_003284"
     CDS             complement(13658..14878)
                     /gene="coaBC"
                     /locus_tag="ACHJ69_003284"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO5016209.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVNRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            15050..15718
                     /gene="radC"
                     /locus_tag="ACHJ69_003285"
     CDS             15050..15718
                     /gene="radC"
                     /locus_tag="ACHJ69_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO5016210.1"
                     /translation="MKNNAQLLMPREKMLKFGISVLTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            15935..16171
                     /gene="rpmB"
                     /locus_tag="ACHJ69_003286"
     CDS             15935..16171
                     /gene="rpmB"
                     /locus_tag="ACHJ69_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO5016211.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            16192..16359
                     /gene="rpmG"
                     /locus_tag="ACHJ69_003287"
     CDS             16192..16359
                     /gene="rpmG"
                     /locus_tag="ACHJ69_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO5016212.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            16457..17266
                     /gene="mutM"
                     /locus_tag="ACHJ69_003288"
     CDS             16457..17266
                     /gene="mutM"
                     /locus_tag="ACHJ69_003288"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO5016213.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(17305..17784)
                     /gene="coaD"
                     /locus_tag="ACHJ69_003289"
     CDS             complement(17305..17784)
                     /gene="coaD"
                     /locus_tag="ACHJ69_003289"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO5016214.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(17792..19069)
                     /gene="waaA"
                     /locus_tag="ACHJ69_003290"
     CDS             complement(17792..19069)
                     /gene="waaA"
                     /locus_tag="ACHJ69_003290"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO5016215.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            19482..20540
                     /gene="rfaQ"
                     /locus_tag="ACHJ69_003291"
     CDS             19482..20540
                     /gene="rfaQ"
                     /locus_tag="ACHJ69_003291"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO5016216.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRNSFTHLVPLQGGNVVESNLSVLTPLGLESLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            20537..21661
                     /gene="waaG"
                     /locus_tag="ACHJ69_003292"
     CDS             20537..21661
                     /gene="waaG"
                     /locus_tag="ACHJ69_003292"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO5016217.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDRVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            21654..22451
                     /gene="rfaP"
                     /locus_tag="ACHJ69_003293"
     CDS             21654..22451
                     /gene="rfaP"
                     /locus_tag="ACHJ69_003293"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO5016218.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEVLKISVIDLHRAQIRAKVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDIIKQEQGLLSQAEEKATKIRERTIRKSL"
BASE COUNT         5323 a         5611 c         6063 g         5556 t
ORIGIN      
        1 ttagttgttt tctgcttaaa atccatgcca tttttctagt ttcagctaag aattcgaacc
       61 aggaaagcat gactcgatac tcgtcatatt tcgccttact gcaaatgcgc gatgaagtat
      121 gacgagtatg aaagagtgat gcggacacaa aggagtaact atgtttcatc tcgatacttt
      181 agcaacgctt gttgccgcaa cgctgacgtt gctgctcggg cgcaagttgg tccattccgt
      241 ctcctttttg aaaaaataca ccataccgga acctgttgcg ggtggtttat tagtggcgct
      301 ggcgctgctg atactgaaaa aaagcatggg ctgggaagtc aactttgata tgtccctgcg
      361 cgatccgttg atgctggctt tcttcgccac cattggcctg aacgccaacc ttgccagtct
      421 gcgagctggt gggcgtgtgg tgggggtttt cctgattgtg gttgttggcc tgttggtgat
      481 gcaaaatgcc attggcattg gtatggctag cctgttaggg cttgatccgc tgatggggct
      541 gctggccggt tctattacgc tttccggcgg tcacggtacg ggtgctgcat ggagtaaatt
      601 gttcattgaa cgttatggct tcaccaacgc gacagaagtg gcgatggcct gtgcaacgtt
      661 cggtttggtg ctgggcggct tgattggcgg tccggtggcg cgctatctgg tgaaacactc
      721 caccacaccg aacggtattc cggatgacca ggaagttccg actgcgttcg aaaagccgga
      781 tgtgggccgc atgatcacct cgctggtgct gattgaaact atcgcgctga ttgctatctg
      841 cctgacagtg gggaaaattg ttgcgcaact tttggctggt actgcttttg aactaccgac
      901 cttcgtctgt gtactatttg ttggcgtgat tctgagcaac ggtctgtcaa tgatgggctt
      961 ttaccgcgtc tttgagcgag cggtatccgt gctgggtaac gtaagcctgt cgttgttcct
     1021 ggcgatggcg ctgatggggc tgaaactgtg ggagctggct tcgctggcgt tgccgatgct
     1081 ggcgattctg gtggtacaga ccatcttcat ggcgttgtat gccatcttcg ttacctggcg
     1141 catgatgggc aaaaactacg atgcggcagt gctggctgcg ggtcactgtg gttttggcct
     1201 cggtgcaacg ccaacagcaa tcgccaacat gcaggcgatc actgaacgct ttggcccgtc
     1261 gcacatggcg ttcctggtcg tgccgatggt cggtgcgttc tttatcgata tcgtcaatgc
     1321 gctggtgatt aagctgtatt tgatgttgcc gatttttacg ggttaaccga tgaagcggcg
     1381 gtcttctcac cgccgccttt tacgcattcg agtagcgttc tgtttctggc atccagcgtt
     1441 ctatcagggc ttttgcctgt tgtgggtaac gttcgtgaat atgtcgcgcc aggcgctgaa
     1501 cttccgggat catcgcctga tcgcgcagta aatccgccac tttaaattca gcattccccg
     1561 tctgacgtgt acctaacaat tcgccagggc cgcgaatctc cagatctttc tgtgcaatca
     1621 caaaaccatc gttgctgtcg cgtagtacct gcaggcggat ttgcgcggtt ttggagagcg
     1681 gcgttttgta gagcagcacg cagtgagagg ccaccgcgcc acgacccacg cggccacgta
     1741 actggtgtaa ctgcgccaga cccagacgct ccgggttttc gataatcatc agactggcat
     1801 tgggtacatc aacaccgact tcaatgacag tagtggcaac cagcaggtgt agctcgcctt
     1861 gtttaaacga tgtcatcacc gcctgtttct cggcgggttt catccgtccg tgtaccaggc
     1921 caacattcaa ctctggtagc gccagtttca actcttccca ggtcgcttcc gccgcctgcg
     1981 cttccagtaa ttccgactct tcaatcaacg tacaaaccca gtacgcctga cgcccttcag
     2041 tcatacaggc gtggcgcacg cggtcaatga tatcgatacg gcgggtatca ggaatagcga
     2101 ccgtagtcac tggcgtgcgg cctggtggca gctcatctat caccgaggta tcgagatcgg
     2161 catacgcagt catcgccagc gtgcggggga tgggcgtggc ggtcatgatc aactgatgcg
     2221 gatggaagcc ctgctgctgg cctttctccc ataacgccag acgctgatgc acgccaaaac
     2281 gatgctgttc gtcgataatc accagcgcca ggccgttaaa ctgcacctgt tcctggaaaa
     2341 tggcgtgcgt accaacaatc atctgcacct gaccgctggc gatggcttct tgctgagcca
     2401 gtcgtgcttt acctttctgt tttccggcga gccagcctac ttcgataccg agcggtgcaa
     2461 accagttacg aaagttattg gcgtgctgct cggcgagtaa ttcggttggt gccatcaggg
     2521 ccacctgttt gccgtgggca accgcacgca acgcggcgag ggcggcgacc agcgttttac
     2581 cggaacctac atcgccctgc accaggcgca tcattggcac atccagcgcc atatcgcgct
     2641 cgatctccgc cactacgcgt gcctgtgcgc ccgttggctt gaacggtaag gcggcgagga
     2701 gtttattttt cagcgcgtca ttggtgctca gcggctgagc atgaaaacgt tgtgcaccgg
     2761 cacgtaacgc caacatgctg aggttgtgcg ccagtagttc ttccagaatc agacggcgtt
     2821 gtgccggatg ctgcccggtt tcgagctcgc taagctgtag cgtcggcggc gggcggtgca
     2881 aagtgcgcaa cgcttccggt agcgtcatca ttccttgtga cagttccggc ggcaggagtt
     2941 cttcaatggc gcatgtgtcg agcagatcca gcgcctggtc ggttaattta cgcagcgtag
     3001 cctgctttac gccttccgta gttggataaa ccggtgtgag cgtttcctgt aactctggcg
     3061 tgctgagatc gccctgtacg cggtattccg ggtggatcat ctccgcacca tatttgccgc
     3121 gctttgcttc gccataggcc agcacgcggc ggcctgtcgc caggctattt ttcattgccg
     3181 cattgaagtt gaaaaagcgc atagtgagaa tgccggaacc gtcgctgatc tggcaggtca
     3241 tcatccgccg accaccgaag gagatattgc agttcagcac ttcgccttcc accgtggcat
     3301 aaacacccgg cagtagttca ccgatggggt agagatgtgt gcgatcttcg tagcgcagag
     3361 gaaggtgtaa gagcaaatcc tgtacggtat gcaggttgat tttcgccagc ttgttactaa
     3421 gcgctgcgcc aacgcccgtt agggaactga gcgggacagc atctaacagg cgacctttca
     3481 tcgcgcttac cctgcggcct gcatggtgga ccaccagtca gcatcggctt caatctcgcc
     3541 ttgctgatta acgtgggggt agggcagacc tttgcgtttt gcgactttcg ccagcaccgg
     3601 atagccgcct tcaaacagca ggcgttgttg ctcggcttcc ggcaacatgc tgttttcacg
     3661 caggtacatg cccgcatttt gccgctggcg ctgggcttcg taaagaatga gggctgaggc
     3721 aacggaaaca ttcagcgact gcaccatgcc gatcatcgga atgatgatat cctgatccgc
     3781 cagggccaac gcttcctgcg tgatgcccgt tttctcctgt cccatcaaaa tacaggtcgg
     3841 gcgagtgtaa tcaatttcgc ggaaatcaac agcgttatca gaaagatggg ttgccagaat
     3901 ctgcatgccc tggcctttca gatgagcgac ggcatcgcca atggtgcggt gcgttttcac
     3961 ctgtacccag ctgttactac ccgcagctgc cgaagccatg gtgcgcatcc ggctgccagg
     4021 ccagacagca tgaacttcat gtacgccaac ggcatctgcg gtacgaataa tcgcagaaac
     4081 gttatgaggt ttgtggacct gctccatgca gacggtcaga tcaggctgcc gcctggcgag
     4141 catttcgcag atgcgtgcat aacgtgttgg gttcataaaa cattaatttc ggtttcgggt
     4201 gactttaatc acgtctggca tcacgcggat tttgcgcatg atattcgcca gatgcacacg
     4261 gtcacgggcg gtcagacgaa taaaggcgct atagacgcga ccatctttct cttccgtatt
     4321 caaactttga atattcgagg tcgtggtgtt aattgccgcc gtcaggtttg ccagcgcgcc
     4381 ctgatgattg aacatctcca ccttgatttc ggtgatgaat tcctgcgccg tctctttatc
     4441 ccattcaaca gccataaact tctctggctc tttctggtag ccacggatgt tacggcagga
     4501 ttcatggtgg atcaccagac ctttaccggg gctgacgtgg gcgataatcg ggtcgccagg
     4561 aattgggcgg cagcatttcg caaaggtgat cagcacgcca tcggcacctt taatgggcag
     4621 atgtccgtga ctttgggttg ccggtggaat ggaggcgtcc ccatgttgca gatttttcgc
     4681 gaccaccacg ctcattgcgt taccaaggcc gatttctgcc agcagatcgt caagcgttgc
     4741 cagcttcatg cggtccagct cgcgctgaat attttcctgc gggatttcat tgagcttacg
     4801 gctaccaccc aacgcatggt tgagcagacg acggcccagg cttacagaat catcacgctt
     4861 gaggtttttc agcaactgac gaattttggc gcgcgctttc gagctgacga caaagttcag
     4921 ccacgcggca ttcgggcgag cacccggtgc ggtaatgatt tcaacggttt gaccgctagt
     4981 aagcggctgc gacagcgggt aaggctggcg gtcgacgcgt gcgcccacgc aggcatggcc
     5041 gatatcggta tgcaccgcat aagcgaagtc gacgggcgtt gcaccggcag gcagttcgac
     5101 aatgcgccct tctggtgtga aaacgtaaat ctcatccggg aagagatcgg atttaacgct
     5161 ctcgataaat tcaaacgagc taccggcact ctgttgcagc tccagcaggc tttgcatcca
     5221 gcgctgggcg cgtatttgcg cggtagtgct ggtttcgccg tgctctttat aagcccagtg
     5281 cgcggcaaca cccatctccg ccatctgatc catatcttcg gtacggatct ggacctcaac
     5341 cggcacgccg tgcgggccaa tcatcgaggt gtgcaaagac tgatagccgt tcgcttttgg
     5401 aatggcgata tagtctttca cgcggcccgg acgcggcttg tacaggctgt gcatctggcc
     5461 cagcacgcga taacaggtgt cagaatcatt gacgatcacg cggaaagcgt agatgtccat
     5521 gattgagtga aaacgctgct ctttgagcac cattttgcag taaatcgaat aaagatgctt
     5581 ttcgcgacca ctgacgcggc acggtattcc cgcttcctgc aaacgccctt cgatttcaga
     5641 aagaatcttc tggatcatct ccttacggtt gccgcgcgcg gctttcacca cttctttgat
     5701 tacgcgataa cggttgggat acagcgcctc aaaacccagc tcctcgagtt cggttttaat
     5761 gtggtggata cctaaacggt gcgccagcgg gctgtatatt tcgagagttt cacgggcgat
     5821 gcggcgacgt ttgtccgggc gaagtgagcc cagcgtgcgc atgttgtggg tacggtcggc
     5881 aagtttgatg agaatgacgc ggatatcctg caccatcgcc ataatcatct tgcgaaagtt
     5941 ttcggcctgc gcctctttct tatcgcggaa cttgagttta tcaagtttcg acaccccctc
     6001 taccagctcg gcgacgcttt taccaaaaag ctgttccatg tcctggtagg tggcgggtgt
     6061 atcttcaatc acatcatgca gcagcgccgc catcagcgtt tcatagtcga gtttcatctc
     6121 ggccagaatg caggcaaccg ccaccggatg cgtgatatag ggttcaccgc ttgaacgtgt
     6181 ttgcccctcg tgagcatcac gtgcaacgag atacgcctgc cgcagacgct tgatttggtc
     6241 ttccggcagg tagttttgaa tcagctgatt caggctttca aacagataca agggcgaccc
     6301 gctttgtgat taacgacgac cttcagcaat agcggtaacg gcttgtaatt cagcggcttc
     6361 ctgctcttgc tgttcctggc gttcgcgaac gtcgaggatc tggttgttga tcagaccttc
     6421 ttcgatttcg cgcagcgcga ttacagtggt tttatcgttt tcttccggta ccagcggatc
     6481 ctttccgcct acctgcatct gacgagcgcg acgcgcggcg accagtacca ggtcaaaacg
     6541 gttaccaatt ttctctacag cgtcctgaac agttacgcgt gccatactta aaaagctcca
     6601 caggtgaaga aatgactggg catgatactg aaatcaggtt cagtctgcca acaatttgct
     6661 gattaaagcg tcatgacgct gcttttggcg gctcatgcgc agacgttcgg cgcgaataat
     6721 ggtcttcaaa tcggtcaacg cggtatcgaa gtcatcattc acaatcaggt aatcatattc
     6781 ggcgtaatgg ctcatttctg caacagcttg cgccatacgc tttgcaatga cctcttcgct
     6841 gtcctgaccg cgaccgcgta gacggcggtc cagttcgatt ttagacggcg gtaaaataaa
     6901 gatactccgc gcgtgcggca tcttctggcg aatttgctgc gcaccctgcc agtcgatatc
     6961 gagaaaaaca tcgacaccgg tcgccagtac ttgctcaatg gcctcacgcg aagtgccata
     7021 gtaattacca aaaacttctg cgtgttcgag gaacgcatct ctactaatca tttctttaaa
     7081 ttcatcatga ttaacaaaga aataatgttc accgtggact tcaccaggac gcggttggcg
     7141 tgtggtgtgt gaaacagaaa cctgggtgtc atacaacggt tgggttttta ataaagcctg
     7201 aatcaggctg gatttacccg cgccactggg ggcagaaaca atataaagcg tgccttgagc
     7261 catgagtatc tttcgtatgt gattagcgaa ataaagccta catacgagct tattatacac
     7321 ggcgcggcca tgtgacgtag cccttgctca cactttttgc gcaaagttgt tgcattttgc
     7381 gtagctgctt ccgcatttgc ctgtgattca ctgcaataaa gacgattctc aggaagcgtc
     7441 ctcgcagact ccgtttttgc cactcggctt tgtcttcacc aagttgtata tctgcctgac
     7501 tatctacagc ggaggtgatg atgaaagtat ggatggcgat attaataagt atcttgtgct
     7561 ggcaatcatc ggcgtgggcg gtctgtccgg cctggtcgcc agccagagca caggaagaaa
     7621 tttcccgcct gcaacagcaa ataaaacagt gggacgatga ctactggaag gaagggaaaa
     7681 gtgaggtgga agacggtgtt tacgatcagt taagcgcccg tctcacgcag tggcaacgct
     7741 gttttgggaa cgagactcgc gatgtgatga tgccgccttt aaacggtgcg gttatgcatc
     7801 cggttgccca tacgggtgta cgcaaaatgg cggataaaaa cgcattaagt ctgtggatgc
     7861 gcgaacgtag cgatctttgg gtgcagccaa aagttgatgg cgttgcggta accctggttt
     7921 atcgggacgg gaaactgaac aaagcaatca gtcgcggcaa tggcctgaaa ggcgaggact
     7981 ggacgcagaa agttcgctta atttccgctg tgccgcaaac cgttagcggg cctttagcca
     8041 acagcacgct tcagggggaa atatttctcc agcgtaaggg gcatatccaa caacaaatgg
     8101 ggggaataaa tgcccgcgca aaagttgctg gcctgatgat gcgccagggg aatagcgata
     8161 cgctaaactc actggctgtt tttgtctggg catggccgga tggaccgcat ttaatgaccg
     8221 atcgtttaaa ggatctggct accgcaggtt ttactttaac gcagacgtat acccgcgcgg
     8281 ttaaaaatgc tgatgaggtt gcgcacgtac gcaatgagtg gtggaaagca aaattaccct
     8341 tcgtcaccga tggcgtggtt gtgcgagcgg cgaaagaacc cgaatcccgt cattggctac
     8401 cgggccaggc ggagtggctg gtggcctgga aatatcaacc tgtagctcag gttgccgaag
     8461 tgaaggcgat tcagtttgcg gtgggtaaga gcggtaaaat atcggtggtt gcgtcacttg
     8521 tacctgtcat gctggatgat aaaaaagtcc agcgggtgaa tattggttcc gtcaggcgct
     8581 ggcaggagtg ggatattgcg cctggtgatc agatcctcgt cagccttgcc ggtcagggga
     8641 ttcctcgcat tgatgatgtg gtctggcgcg gagcagaacg tacaaaaccg acaccaccag
     8701 aaaaccgctt taactcgttg acctgctact ttgcttctga tgtttgtcag gaacagttca
     8761 tttcacgctt agtctggctg gggtcaaaac aggttctggg gcttgatggc attggtgagg
     8821 ccggctggcg cgccctgcat cagactcatc gctttgagca tatcttttcc tggcttttat
     8881 taacgccaga gcaattacag aacacgccgg ggatcgcgaa aagtaaaagt gcgcagctat
     8941 ggcatcagtt taatctggct cgtcagcagc cttttactcg ctgggtgatg gcaatgggaa
     9001 taccgctaac ccgggcggcg cttaatgcca gtgatgaacg gtcctggtcg caacttttat
     9061 ttagcacgga acagttctgg cagcagctgc cggggactgg atcggggcgc gccagacagg
     9121 ttattgaatg gaaggaaaat gcgcaaatca agaagctggg cagttggctg gctgcccagc
     9181 agatcacagg ttttgaacct tagtgacctt cgtggctgta gtaaaagacc ggtaatccca
     9241 gcttaagacg tagcgccagt aaacgggcaa aaaagccgaa taccaaggtg gagataatca
     9301 ccacatcatg gttagaaaca tagtgttgca gcgcgatgta cagcacggcg gaggcaaatg
     9361 agacaccggc atacaactct ttctggaata ccagcgggat gcgtttacag aacatatcgc
     9421 gcaaaacgcc gccaaacacg ccagtcgtca cagccgcgac aacggcaatg atcgggccgt
     9481 gtcccatatc cagcgcgacc tgtgcgccga tgatagaaaa gaccaccagt ccgagtgcat
     9541 cgagcaccag aaacactttg cgcagataag gcattacagg agcaactata gtggtgagta
     9601 ctgcagcggt agcgacgata atcacatatt ccgggtgttt gacccagccg agcggatagt
     9661 ggcccagcag aatatcgcgc actgaccctc cgccaattgc cgtggcggtc gcaataataa
     9721 ttacgccaaa tgtatccata cgccgtcgtc cggccgccag cgcccccgtc atggcttcgg
     9781 cagtaatgcc aaccaaatac aaaatgtgta acagcatggt tctctcccta aaaatctgtc
     9841 tggaagggta gcgatttgtg cgcattgtca cgattgagat tttttaagga gagaatagat
     9901 tgataatttg tagttatgtg aaattgctat tttctgtagt aagaatgcat ttttctgatt
     9961 ttgtgataag taaaatgcat tagaaaaagt atggagtaaa taaatatgag gacgaaacta
    10021 tatgcgtagc gccgctgtaa ttaacaacgg cctacggaga tatgtcgctt actcaatatt
    10081 ctgaatctgc tcgcgcatct gctcaatcaa cactttcagc tcgatggcgg agtttgtcac
    10141 ttcggcgttg atagatttcg acgcaagagt gttcgactcg cggttgaact cctgcatcat
    10201 aaagtccaga cgacgaccaa cggcttcttt tttcttcaga atgttgtagg tctctttgac
    10261 atgcgcttcg aggcggtcca gttcttcggc aacgtcaatt cgttgtgcca gcagaaccaa
    10321 ctcctgctcc agacggttgt tttccagttg cacctgagca tcttccagct tcgctatcag
    10381 acgctcacgc tgccattgca ggatttccgg catatgggcg cggactttgg ccacttcggc
    10441 ggtgacgcct tccagacgct gctcaatcaa cactttcagt gcctgacctt cggtttcgcg
    10501 cgcgatgata aagtcatcca gggtgccatc caacgccgcg agaatttcag ctgcaatcgc
    10561 gtcgagatct tgctcctggg ctgccatcac gcccggccag cgtagaatat caaccgggtt
    10621 gatttctcct tcgtcgctct gcatttttac ccagttcgcg gcagttacca gctgtttagc
    10681 cagtttttcg ttgaggatca gctcaccttg cgcgctaaca tctggctcat agcgcagagt
    10741 acattccact ttaccgcgcg tcaggcgaga acgaatacgc tcgcgaacga caggttcaag
    10801 gctacggaac tgctccggca gacgaaagta agtttccaga taacgctggt ttaccgagcg
    10861 catttcccag gttgcgctcc cccattcacc cttgatttca cgccgggcgt aggcggtcat
    10921 actgcggatc atagacgttc ctgtttataa aaggagaggt ggaaggatta tagccatcga
    10981 tgccttgtca ggataggaat aaccgccgga agtccgtata atgcgcagcc acatttgttt
    11041 caagccggag atttcaatat gcgtccagca ggccgtagca ataatcaggt gcgtcccgtt
    11101 accctgactc gtaactatac aaaacatgca gaaggctcgg tgctggtcga atttggcgat
    11161 accaaagtat tgtgtaccgc ctctattgaa gaaggcgtgc cgcgcttcct gaaaggtcag
    11221 ggccagggct ggatcaccgc agagtacggt atgctgccac gttctaccca tacccgtaac
    11281 gcgcgtgaag cggcgaaagg taagcagggt ggacgcacaa tggaaatcca gcgtctgatc
    11341 gcccgtgctc ttcgcgcggc agtagatttg aaagcactgg gtgagttcac cattacgctg
    11401 gactgcgacg tgcttcaggc tgatggtggc acgcgtaccg cttcgattac gggtgcctgc
    11461 gtggcgctgg cagatgcgct acagaagctg gtggaaaacg gcaagctgaa aactaatcca
    11521 atgaaaggga tggtagccgc ggtctctgtc gggattgtga acggtgaagc ggtttgcgat
    11581 ctggaatatg ttgaagactc tgccgcagag accgacatga acgtagtgat gaccgaagac
    11641 gggcgtatca ttgaagtgca ggggacggca gaaggcgaac cgttcaccca cgaagagtta
    11701 ctcaccttgt tggcgctggc ccgaggggga atcgaatcca ttgtagcgac gcagaaggcg
    11761 gcgctggcaa actgattttt aaggcgactg atgagtcgcc ttttttttgt ctgtagaaaa
    11821 gtaagatgag gagcgaaggc atgaaaccat atcagcgcca gtttattgaa tttgcgctta
    11881 gcaagcaggt gttaaagttt ggcgagttta cgctgaaatc cgggcgtaaa agcccctatt
    11941 tcttcaacgc cgggctgttt aataccgggc gcgatctggc actgttaggc cgtttttacg
    12001 ctgaagcatt ggtggattcc ggtattgagt tcgatctgct gtttggccct gcttacaaag
    12061 ggatcccgat tgccaccaca accgctgtgg cactggcgga gcatcacgac ctggacctgc
    12121 cgtactgctt taaccgcaaa gaagcaaaag accacggtga aggcggcaat ctggttggta
    12181 gcgcgttaca aggacgcgta atgctggtgg atgatgtgat caccgccgga acggcgatcc
    12241 gcgagtcgat ggaaattatt caggccaatg gcgcgacgct tgctggcgta ttgatttcgc
    12301 tcgatcgtca ggaacggggg cgcggcgaga tttcggcaat tcaggaagtt gagcgtgatt
    12361 acaactgcaa agtgatctct atcatcaccc tgaaagacct gattgcttac ctggaagaga
    12421 agccggaaat ggcggaacat ctggcggcgg ttaaggccta tcgcgaagag ttcggcgttt
    12481 aaagaaactc gccggatgaa aagtcatccg gcgtcatatt actgcaactg cgcggcaatt
    12541 agcggccagc gggcgtcaaa atcatccgtc gggcggtatt taaattcgct gcggacaaaa
    12601 cgtgacagca taccttcaca gaaggccagg atctggcttg ccagcagggt ttcatcggtg
    12661 gtgtaacctt caccctcacg cattctcttt tcacgcaata cctgacgcag ctgcgcttca
    12721 atacgctcga aaagttggtt gatgcgccct tgcaggcggt cttgttcaaa catcagcgca
    12781 tgcccggtaa ggatgcgggt caggccagga ttacgctcac caaaaccgag aagcagcaac
    12841 acaatcagac gcaggcgtgc tgtggtgtct ttctcatctt tcagaatcag attgatgcga
    12901 gtaatcaggc tatcttcgat aaactcaatc aggctatcga acatgcgggt cttactgggg
    12961 aagtggcgat acagtgccgc ttcggaaacg ccgacagagg cggccagttt tgccgtcgtg
    13021 atacgttggc ttccatcgct ggattccagc atcagcgcca gagactgaag tatttcctcg
    13081 cgacggttcc ttttcgcagt ttgtttttct gccatgttac aaaatacccc tgaaaataag
    13141 cacttgccag gcggcaccca cgctatgacc gcaaacgaaa tgtttgcggc tatgttatga
    13201 cgttattcgg atgcgtatgt gttactgacg accagagtga ccaaagccgc cttcaccgcg
    13261 gtcggtggcg tcgaaatctt ccaccagatt aaattcagcc tgtactaccg gaacaaaaat
    13321 catctgggcg atgcgttcgc caggttgaat ggtgaagctg tcctgaccac ggttccacac
    13381 ggaaatcatc aactggccct gatagtcaga atcgatcaat cctaccaggt taccaagcac
    13441 gataccgtgc ttatgtccca atccggagcg cggcagcatc attgccgcca gtgaaggatc
    13501 ggcaatatga atcgccagcc cggtcggaac cagcgtagtg tcacccggag ccagttctac
    13561 ggcgtcgtcg agacaggcac gcaggtcaag tccggcagag ccagaggtgg cataagtcgg
    13621 gagcggaaat tccttcccaa cgcgcgggtc cagaatctta acgtcgattt ttttcatcat
    13681 aacgggtcac gatctcgtcg agtaataatt ggccaaggag ctctttgcgc tcaagcggta
    13741 agactttatc tccgtcctgc cagaaaaggt gtaatgcgtt gttgtcgctg ttaaatcctt
    13801 gagttggctg ggaaacatcg ttcgcgcaga tcagatcaag gtttttacgg atacgttttt
    13861 gccgggcgta ttcttccaca ttatttgttt cggcggcaaa tccaacgacg tagggtcgat
    13921 ggtcttttag tgcggcaacg cctgcgacga tatcggggtt tttaaccatt tttattgtta
    13981 attcatcacc ctgcgtggcc tgctttttga ttttttctgg ggccacggta gctgcgcgat
    14041 aatccgccac ggcggcgcaa ccgataaaaa tattttgctg ctgtacagaa gcattcacgg
    14101 cggcttccat ttccagcgcg gtcatcacat caacacggtt aacaaacggt ggcgtcggta
    14161 gtgaaaccgg acctgatacc agcgtgacgt tcgcgccacg acgggctgcg gcggcggcga
    14221 tagcaaaacc catcttgccg gagctgtgat tagagatata acgcacggga tcgagcggtt
    14281 cacgcgtcgg accggcggta atcataatgt tcagatgttt caggtcgttg acgggcgaaa
    14341 aatgcgctac cgccatatcc acaatagtta acggatcgag cattcgtcct gggccgatat
    14401 caccacaagc ctgactgccg ctgtctggcc cccagatgag caaaccacgt gaggcaagca
    14461 cctctaaatt atgctgcgtg gcagcggcac ggtacatctg ctggttcatg gcggggagca
    14521 cggctacagg cgcaggtgta gccagacaaa tcgtcgatac caggtcattc gccattccgg
    14581 cagcaacacg ggcaattaaa tctgccgtgg caggggcgag aatcactaaa tcagcccatt
    14641 tacccagctc aatatggccc atagcggctt cggctgccgg gtccagcaga ctgtcggaaa
    14701 cgggataacc agaaaccgcc tgcaagctaa gtggggtgat aaaggctttt gccgcttcgg
    14761 tcatggctac gcggacatcg gccccgcgat cgcgcaaacg acgcaccagt tcaggggttt
    14821 tataggcagc aataccgccg ctaacgccga gaacgatttt tttaccggcc aggctcatga
    14881 tgatttttcc tgttgggtga caccagaagt tggcgatttt atcacaatac ttttgttgtc
    14941 gtgccttcac ctgagattca ctttgcgagg cgctttccag gattgaaaac tggccgtcga
    15001 tttaaaggaa cggctatgac aggatgcgag caccacaaag gaggtgaagg tgaaaaacaa
    15061 tgcacagctg ttgatgccgc gcgaaaaaat gctgaagttt ggtattagcg tcttaacgga
    15121 tgtcgagctg ctggcgctat ttctgcgtac cggaacgcgc ggtaaagatg tattaaccct
    15181 ggcaaaagag atgctggaga atttcggctc tctttatggc ttgttaacct ctgaatatga
    15241 gcaatttagt ggcgttcatg gaattggcgt ggcgaaattc gcccagttaa aggggattgc
    15301 cgaactggcg cggcgttact acaacgtacg gatgcgtgaa gaaagccctt tactcagccc
    15361 ggagatgacg agggaatttt tacaaagcca gctcacgggc gaggagcggg agatctttat
    15421 ggtgatcttt ctcgactccc aacacagggt tataacgcat agccgtcttt tttccggcac
    15481 gctaaaccat gttgaagtcc atcctcggga aattatccgc gaagcgataa aaataaacgc
    15541 ctcggcgctg atccttgcgc ataatcaccc ttcgggttgt gctgaaccca gtaaagcgga
    15601 taaactcatt actgaacgga taataaagag ttgtcagttc atggatttac gcgtgctcga
    15661 ccatatcgtg attgggcgtg gagagtatgt ttcttttgcc gaacgcggct ggatttaacc
    15721 cgctatgcgc gatccttcgg gatctttgtc tgttcgggac ttgagcacat cgctgagtca
    15781 gcgtatacta cgccaccttt gagaatctcg ggtttggcat ttgggcctgg caatcgagag
    15841 ttcacataga actgcgatga ccgggctgta aagcctgacg aggcgccaat accccatacg
    15901 aagctcgagc taatttgatt tttggagaat agacatgtcc cgagtctgcc aagttactgg
    15961 caagcgtccg gtgaccggta acaaccgttc ccacgcactg aacgcgacta aacgccgttt
    16021 cctgccgaat ctgcactctc accgtttctg ggttgagagc gagaagcgtt ttgtcaccct
    16081 gcgcgtatct gctaaaggta tgcgtgtaat cgataaaaaa ggcatcgata cagttctggc
    16141 tgaactgcgt gcccgtggcg aaaagtacta agtacttaga ggaaataaat catggctaaa
    16201 ggtattcgtg agaaaatcaa gctggtttct tctgctggta ctggtcactt ctataccact
    16261 acgaagaaca aacgtactaa gccggaaaaa ctggaactga aaaaattcga tccagttgtt
    16321 cgccagcacg tgatctacaa agaagcgaaa atcaaataat tctcgctttg atgtaacaaa
    16381 aaacctcgcc ccggcggggt tttttgttat ctgcttgccc ccatattgac tgcatctgtt
    16441 cattcctgga gatgctatgc ctgaattacc cgaagttgaa accagccgtc gcggcataga
    16501 accgcatctc gttggtgcaa ccattcttca tgcggtggtg cgcaacggac gcttgcgctg
    16561 gccggtttca gaagagatct accgtttaag cgaccaacca gtgcttagcg tgcagcgccg
    16621 ggctaaatat ctgctgctgg agctgcctga gggctggatt atcattcatt tggggatgtc
    16681 tggcagcctg cgcatccttc cagaagaact tccccctgaa aagcatgacc atgtggattt
    16741 ggtgatgagc aacggcaaag tgctgcgcta caccgatccg cgccgctttg gtgcctggct
    16801 atggaccaaa gagctggaag ggcacaacgt gctggcccat cttggaccgg agccgcttag
    16861 tgacgatttc aacggtgagt acctgcatca gaagtgcgcg aagaaaaaaa cggcgattaa
    16921 accgtggctg atggataaca agctggtggt aggcgtaggg aatatctatg ccagcgaatc
    16981 gctgtttgcg gcggggatcc atccggatcg gctggcgtca tcactgtcgc tggcggagtg
    17041 tgaattgtta gctcgggtga ttaaagcggt gttgctgcgt tcgattgagc agggcggtac
    17101 aacgctgaaa gattttctgc aaagtgatgg taaaccgggc tatttcgctc aggaattgca
    17161 ggtttacggg cgaaaaggtg agccgtgtcg ggtgtgcggt acgccgattg tggcgactaa
    17221 acatgcgcag cgggcaacgt tttattgtcg gcagtgccag aagtaattga tgcgcgccgg
    17281 atggcatacc atccggcata aactctacgc taacttcgcc atcagcgcct gatggacatt
    17341 ctccggcagg aaatgggtga catcgccctg atggcgcgcc acctctttca ccaacgatga
    17401 agagataaac gaccactctt tcgacggcat cagaaacaca ctttccagct ccggcattaa
    17461 gtggcgattc atatgcgcca gctgcatttc atattcaaaa tccgccaccg cacgcaggcc
    17521 acgaatcagc accgtagcgt gttgattacg ggcgaagttc gccatcaaat cactaaaccc
    17581 gaccacttcc acgttcccca gatgtgcggt tgcctgctgt gccagttcca cacgctcttc
    17641 cagggtaaac atcggttttt tactggggct agcggcaatc gccagaataa cgtgatcgaa
    17701 catttgcgtg gcgcgcgtca cgatatcgat atgaccattg gtaatgggat cgaaagtacc
    17761 cggataaatc gcccgttttt gcataacaac ctcaatgcgt tttcggtggc aggtaaggtt
    17821 ccagcagttg aagcagacgc tgtagtgcgc cctggttttg atacagcact tcaacggcat
    17881 gacggccata gaaactacgg taatcggcgt cggtgagtaa agaggaaacc tcttttgcaa
    17941 gcgtagtggc atcggtaacg gtaatcagcc cgcttgcctg ttccagccgc gcgcaaatgt
    18001 ctttaaagtt aaacgtatgc ggccccatca ataccggaat agcgtgtgcg gcagcttcca
    18061 gcggattatg cccaccacgt tcaaccagtg aaccgccaac aaaggcgaga tcggcaatgc
    18121 cgtacagtaa catcaactcg cccatcgtat cgccaaccac aacctgcgtg ctggtggagg
    18181 ggacttcccc tgaagatcgt gtgatatagc ttagtccagc ctggcggaca aggttaatcg
    18241 catccgggaa gcgttccgga tgacggggta ccaggatgag caataaattc gggaattgct
    18301 gtaacaatgc ctgatgtgcg gcgatcacca cactctcttc gccttcatga gtgctggtgg
    18361 caatccatac cgggcggtgt ggtgcccatt ggcggcgcag cgtcacggct ttagcagcca
    18421 actgcggcgt tacagaaata tcgaatttca ggctaccggt aacggtcacc tgattatttt
    18481 ttgcgcccaa cgccacaaaa cgagcaccat cttcttcatt ttgggcggca atcagcgtaa
    18541 tacgacgcag caagcgacgg acgaatttac ccagtttggc ataacctgcg gccgagcggg
    18601 cagagagtcg cgcgttagcg atcaccagcg gaattttacg tttatgtagc gccgcaatca
    18661 ggttaggcca tagttcggtt tccataatca acaccagttt agggtcgact ttattcagga
    18721 aacggttgag tgcatcgggc aggtcatacg gcagataaac gtgctgaaca tccttcccga
    18781 aagccgattg tacgcgctcc gaaccggttg gagtcatggt tgttacggta atcggtaaat
    18841 caggataacg atgacgcagt gcgcgcacca acgggatcgc cgccagagtt tcaccgacgg
    18901 agacggagtg cagcataatg ccgcctggtt ttagcggatg gcggtaaaaa ccgtaacgtt
    18961 caccccagcg ttttcgatag gccggagcct tacgtccgcg cacccagagc cgtatccaga
    19021 tcagcggctg aataaggtag agaagggcgg tgtaaagcaa ttcgagcata gtaaatagct
    19081 gacttatgga tgtgctgggg attctatgta tttagctgtg gctttaccat tacttttccc
    19141 atttttgact taaatagcaa tttacccgcc aattcttgct gaacatctat actttttttg
    19201 tatttttatg cgactcattg atttctgcag tacgtgcagg aaggaatcgc agagcaacag
    19261 taaaagaata tggttgctaa tgattttatt tgattgattt ctttattgaa aatcaacaat
    19321 gtgcttaaaa atcattcgag tcggattgcc gcgaaagaag tgatacacta gcgcgtcaat
    19381 atcagtgcgg tatcagtatt caggtagctg ttgagcctgg ggcggtagcg tgctttttct
    19441 gcttcacctt aatcggataa tctcaacaaa aagagttact tgtggataag ccatttcgaa
    19501 gaattttgct cattaagatg cgttttcatg gggatatgtt attaactact cccgtcatta
    19561 gttcgctgaa aaaaaattac cctgacgcaa aaatcgatgt gctgctttat caggacacca
    19621 tcccgatcct gtctgaaaat ccagagatta acgcgctcta cggcataaaa aataaaaaag
    19681 caaaagcctc agaaaaaatt gccaactttt ttcatctcat caaggtatta cgtgccaata
    19741 agtatgacct tatcgttaat cttaccgatc aatggatggt tgctatactg gttcgcttat
    19801 taaatgcccg tgtgaaaatt tcccaggatt atcatcatcg gcagtctgct ttttggcgta
    19861 atagtttcac tcatttggtg ccgttgcagg gtggaaatgt ggtggaaagt aacttatccg
    19921 tgctgacgcc attgggactt gaatcgttgg tgaagcagac aaccatgagt tacccgcctg
    19981 caagctggaa acgtatgcgt cgcgaacttg atcacgctgg tgttggacaa aattatgtgg
    20041 ttatccaacc tacggcgcgg caaatcttca aatgttggga caacgccaag ttttccgctg
    20101 tgattgatgc cttacatgct cgtggttatg aagtcgttct gacgtccggc ccagataaag
    20161 acgatctggc ctgcgtcaat gaaattgcgc agggatgcca gacgccacca gtaacggcgc
    20221 tggccggaaa ggtgaccttc ccggaacttg gtgcgttaat cgatcatgcg cagctgttta
    20281 ttggcgttga ttccgcaccg gcgcatattg ccgctgcagt taatacgccg ctgatatcgc
    20341 tgtttggtgc gacagaccat attttctggc gtccctggtc aaataacatg attcaattct
    20401 gggcgggaga ttaccgggaa atgccaacgc gcgatcagcg tgaccgaaat gagatgtatc
    20461 tttcggttat tccggcggca gatgtcattg cagctgtcga taaattactt ccctcctcca
    20521 cgacaggtac gtcgttatga tcgttgcttt ttgtttatat aaatattttc cctttggcgg
    20581 tttgcagcgc gattttatgc gtattgctca gacagtcgcc gcccgaggtc atcatgttcg
    20641 ggtttatacc cagtcgtggg aaggcgaatg ccctgatgta tttgaactga tcaaagtgcc
    20701 ggttaaatcg cataccaatc acgggcgcaa tgcggaatat tttgcctggg tgcaaaaaca
    20761 tttacgtgaa caccccgtcg atagagtcgt tggattcaac aaaatgccgg ggctggacgt
    20821 ttattatgcc gctgatgttt gttatgccga gaaagtagcg caggagaaag gctttttcta
    20881 tcgcctgacg tcacgttatc gccattatgc cgcctttgag cgggcaacct tcgaacaggg
    20941 caagccgaca cagctgctga tgctgacaga taagcaaatc gccgatttcc agaaacatta
    21001 tcagactgaa gcggagcgtt ttcatattct gccaccgggg atttatcctg atcgtaaata
    21061 tagccagcaa ccagcgaata gccgtgaaat cttccgtaag aagaatggaa taaccgaaca
    21121 acaatattta ttgttgcagg tcggttcaga cttcacgcgt aaaggtgtcg atcgttccat
    21181 tgaagcactt gcttcgttac cggattcgct gcgccacaac acattactat atgttgttgg
    21241 gcaggataaa ccgcgaaaat ttgaggcgct ggcagaaaaa cgcggcgtgc gcagtaatgt
    21301 tcacttcttc tcggggcgca acgatgtctc ggaattaatg gcggcggcgg atttattact
    21361 tcatcctgcc taccaggaag ccgcaggaat tgtactgctg gaagcgataa ctgcaggatt
    21421 accggtacta acaacagccg tttgtggcta tgcgcattat attgtcgatg ccaattgcgg
    21481 cgaggctatt gctgagccat tccgccagga aacattgaat gagattttac gcaaagcgtt
    21541 aacgcaatct tcattgcgcc aggcctgggc ggaaaatgcg cgacattatg ctgatacaca
    21601 agatttatac agtctgccag agaaagcggc ggacatcata acgggtggtc tggatggttg
    21661 aacttaaaga gccgtttgcc acgttatggc gcggtaaaga tccttttgag gaagttaaaa
    21721 ccttgcaggg tgaggtattt cgtgaactgg aaactcgccg cactctgcgc tttgaaatgg
    21781 cgggcaaaag ctattttctc aaatggcatc gcggcacgac cctgaaagag ataatcaaaa
    21841 atttactctc attgcggatg ccagtattag gcgcagaccg cgaatggaat gcgattcatc
    21901 gactgcggga tgtcggcgtt gatactatgt atggggtggc attcggcgaa aaaggcatta
    21961 atccgctgac cagaacctcg tttattatta ccgaagatct gacaccaacc atcagtctgg
    22021 aagattactg tgctgactgg gcgaccaacc caccagatgt tcgcgtaaag cgtatgctta
    22081 ttaagcgtgt cgcgacgatg gtgcgcgata tgcatgctgc gggcattaac caccgtgact
    22141 gttatatctg tcatttcctg ctgcacttgc ctttttccgg taaggaagag gtgttaaaaa
    22201 tttcagtaat tgacctgcac cgggcgcaga tacgtgcaaa agtaccgcgt cgttggcggg
    22261 ataaagatct tattggactt tatttttctt cgatgaatat tggcctgact cagcgggata
    22321 tctggcggtt tatgaaagtg tattttgtcg ccccgcttaa agacattatc aagcaggaac
    22381 aagggctgct gtcgcaagca gaagaaaaag ccacaaaaat cagggaaaga acgattcgaa
    22441 aatcgttgta acccgtaact tatttttgcc aaaattttgg atacagaata aatatgtctc
    22501 aactcaatga tagtgacatc atcctttttg agtataaatt tcattatcaa aat
//
LOCUS       DAZFFE010000081        21770 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFFE010000081 DAZFFE010000000
VERSION     DAZFFE010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.081"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..472
                     /locus_tag="ACHJ69_003294"
     CDS             <1..472
                     /locus_tag="ACHJ69_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO5016219.1"
                     /translation="STWQGVVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQALWA
                     RRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVI
                     HRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA"
     gene            complement(515..679)
                     /locus_tag="ACHJ69_003295"
                     /pseudo
     CDS             complement(515..679)
                     /locus_tag="ACHJ69_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(664..1890)
                     /locus_tag="ACHJ69_003296"
     CDS             complement(664..1890)
                     /locus_tag="ACHJ69_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO5016220.1"
                     /translation="MPVTITKIRTGEDVLSAAIHRIEWLFETFSSVCLSFSGGKDSTV
                     LFHLVAEVAYRKKRRFSVLFIDWEAQYQCTIDHIQKMRMMYQDVTDTFYWVALPLTTV
                     NGVSQFQPEWICWEPGVEWVRQPPEDAITDMAYFPFYRYAMTFEEFVPAFSSWFASNQ
                     CSVAILTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTMYPLYDWKTNDI
                     WVYNAKTCAIYNPLYDLMYRAGIPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARMCE
                     RVSGAASGALYANESGAYFALRKRMSKPSHHTWRSYAFFLLDVMPERTAEHYRNKIAV
                     YLRWYQNRGFPDDIPDEQENDLGCRDVPSWRRICKTLIKNDFWCRTLSFSPNKPRHYE
                     RYLQRMNERRKEWGIL"
     gene            complement(2087..2716)
                     /locus_tag="ACHJ69_003297"
     CDS             complement(2087..2716)
                     /locus_tag="ACHJ69_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFO5016221.1"
                     /translation="MTTQLIPVFEGTISNEPTLLVNARDLHGFLEVDKDFSNWIRARL
                     NEYGFVENLDYILFSPNLAKTLGRRRKDYHLTLDTAKELAMVERNEKGRQIRRYFIEC
                     EKKLRNIQPVQAESQPQFTAEEIILLCYMQLWMEKAQDLSKQLYPIMKELNSSYTNKL
                     YDIAFETIYMVTKNRDALLREVTRLDMSSSVIQRAMPMLKSLRARQFEF"
     gene            complement(2997..3434)
                     /locus_tag="ACHJ69_003298"
     CDS             complement(2997..3434)
                     /locus_tag="ACHJ69_003298"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO5016222.1"
                     /translation="MKNLLNNFPDNCIENILPKDDFGYGRLLIGSKGKRIKTGAHRLI
                     YRIFIGEIPAGMVVRHSCDNPACCNPKHLLIGTILDNNADRQKRKRQARHEKHGRAKL
                     TKEQVLFIRANEMSIPNSVLADKYRVSNSLIWQIKNYRIWNEL"
     gene            4250..4423
                     /gene="ymdF"
                     /locus_tag="ACHJ69_003299"
     CDS             4250..4423
                     /gene="ymdF"
                     /locus_tag="ACHJ69_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO5016223.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(4506..5834)
                     /gene="rutG"
                     /locus_tag="ACHJ69_003300"
     CDS             complement(4506..5834)
                     /gene="rutG"
                     /locus_tag="ACHJ69_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO5016224.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKNVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNSVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(5855..6349)
                     /gene="rutF"
                     /locus_tag="ACHJ69_003301"
     CDS             complement(5855..6349)
                     /gene="rutF"
                     /locus_tag="ACHJ69_003301"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO5016225.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPVC"
     gene            complement(6360..6950)
                     /gene="rutE"
                     /locus_tag="ACHJ69_003302"
     CDS             complement(6360..6950)
                     /gene="rutE"
                     /locus_tag="ACHJ69_003302"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO5016226.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(6960..7760)
                     /gene="rutD"
                     /locus_tag="ACHJ69_003303"
     CDS             complement(6960..7760)
                     /gene="rutD"
                     /locus_tag="ACHJ69_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO5016227.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLDQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSRHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(7768..8154)
                     /gene="rutC"
                     /locus_tag="ACHJ69_003304"
     CDS             complement(7768..8154)
                     /gene="rutC"
                     /locus_tag="ACHJ69_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO5016228.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(8166..8858)
                     /gene="rutB"
                     /locus_tag="ACHJ69_003305"
     CDS             complement(8166..8858)
                     /gene="rutB"
                     /locus_tag="ACHJ69_003305"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO5016229.1"
                     /translation="MTTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARTAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRNQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSSTSFARIA"
     gene            complement(8858..9949)
                     /gene="rutA"
                     /locus_tag="ACHJ69_003306"
     CDS             complement(8858..9949)
                     /gene="rutA"
                     /locus_tag="ACHJ69_003306"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO5016230.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVITDETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVANVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMLCRAHLPALTQEVA"
     gene            10237..10875
                     /gene="rutR"
                     /locus_tag="ACHJ69_003307"
     CDS             10237..10875
                     /gene="rutR"
                     /locus_tag="ACHJ69_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO5016231.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(10915..14877)
                     /gene="putA"
                     /locus_tag="ACHJ69_003308"
     CDS             complement(10915..14877)
                     /gene="putA"
                     /locus_tag="ACHJ69_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO5016232.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQSLPMLEQSVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGMMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRQVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPALIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            14932..15141
                     /locus_tag="ACHJ69_003309"
     CDS             14932..15141
                     /locus_tag="ACHJ69_003309"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016233.1"
                     /translation="MMLQLCATVLNVTCVASLKMNEMLINEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            15300..16808
                     /gene="putP"
                     /locus_tag="ACHJ69_003310"
     CDS             15300..16808
                     /gene="putP"
                     /locus_tag="ACHJ69_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO5016234.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            17028..17858
                     /gene="efeU"
                     /locus_tag="ACHJ69_003311"
     CDS             17028..17858
                     /gene="efeU"
                     /locus_tag="ACHJ69_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO5016235.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFVLPPRAGATASRSV
                     "
     gene            17916..19043
                     /gene="efeO"
                     /locus_tag="ACHJ69_003312"
     CDS             17916..19043
                     /gene="efeO"
                     /locus_tag="ACHJ69_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO5016236.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADIPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFENYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            19049..20320
                     /gene="efeB"
                     /locus_tag="ACHJ69_003313"
     CDS             19049..20320
                     /gene="efeB"
                     /locus_tag="ACHJ69_003313"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO5016237.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGVAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNVKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMLHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMELLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            20951..21739
                     /gene="phoH"
                     /locus_tag="ACHJ69_003314"
     CDS             20951..21739
                     /gene="phoH"
                     /locus_tag="ACHJ69_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO5016238.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
BASE COUNT         5218 a         5524 c         5787 g         5241 t
ORIGIN      
        1 cagcacatgg cagggggtcg tctatgtggc gttcatcatt gatgtgtttg ccggatacat
       61 cgtggggtgg cgggtctcat cgtctatgga aacgacattc gtgctggatg ctctggagca
      121 ggcgttatgg gcccgtcgac cgtccggcac agtccatcac agtgataaag gttctcagta
      181 tgtatcgctg gcctacacac agcggcttaa ggaagccgga ttactggcat caacaggaag
      241 tactggtgac tcgtatgaca acgcgatggc ggagagcatc aatggccttt acaaagcgga
      301 ggtaatacac cgtaagagct ggaaaaaccg tgcagaagtg gaactggcca cactcacgtg
      361 ggtggactgg tataacaatc gacgattgct ggaaaggctg ggccatactc ctccggcaga
      421 agcagaaaaa gcttattatg cttccatcgg aaacgatgat ctggcagcct gagttcacag
      481 ataaaacact ctccaggaaa cccggggcgg ttcacagcac acagtccact ggttcttgag
      541 caaaggggct gatctcatgg agtacgcagc ggagatggtt cagggcctgt atggttttct
      601 cttcagattg ttcctgactg aagtactgtc gaatcatctt gctcagcacc tccacctctg
      661 gtgtcacaga attccccatt ccttcctcct ttcattcata cgctgcaggt aacgttcata
      721 gtgccggggt ttgttggggc tgaaggagag cgttcggcac cagaaatcat tctttatgag
      781 agtcttacaa atacgtcgcc aggagggaac atcccggcag cccagatcat tttcctgttc
      841 atccgggata tcatccggga atccccggtt ctgataccag cgcaggtaga ctgctatctt
      901 gttgcggtag tgttcagccg ttctttccgg cattacgtcc agcaggaaga acgcatagct
      961 gcgccaggta tgatgggatg gttttgacat ccgtttacgc agggcaaaat aggccccgct
     1021 ttcattggca taaagagccc cactggcagc accagatact cgttcacaca tccgtgccca
     1081 agtttctggc tccagaacat gataaagcca tagtccctta cgctgttccg ggccaaaagg
     1141 ctcacagaca cgcatattac gtagaggaat gcctgcacgg tacatcaaat catacagagg
     1201 attgtagatt gcacaggttt tagcgttata tacccagata tcgttggttt tccagtcata
     1261 cagcggatac atggtgtagt aaaaaccttc aggtgacgcc gtggtccagg gtttatcatc
     1321 tgcatatctc agttttcgtt gggaaaccag tcccataaat ctgttaagag attcatccgc
     1381 gcggactcca gtcagtattg ccacgctaca ttggttactt gcgaaccagg aagaaaaagc
     1441 tgggacaaat tcttcaaaag tcatggcgta gcggtaaaag gggaaatacg ccatatcagt
     1501 aatagcatct tccggtggct gtcgaaccca ctctacaccg ggctcccagc atatccactc
     1561 aggctgaaac tgcgagacac catttactgt cgtcagggga agtgcaaccc agtaaaaggt
     1621 atctgttaca tcttggtaca tcatccgcat cttctgtata tgatcaatgg tgcattgata
     1681 ctgggcttcc cagtcaatga acaatacaga aaaacgacgt ttcttcctgt aggccacttc
     1741 ggctacaaga tggaaaagta cagtggaatc tttaccgcca gaaaaagata aacatacaga
     1801 agagaatgtt tcaaacaacc attcaatgcg gtgaatggct gctgacaaaa catcttctcc
     1861 agttctgatt tttgttatag ttaccggcat acaatctgat aaaaatagcg agggtttgca
     1921 ttttacctag taacgttatg taactatttt tttgaataaa ggcaatgaga taatgataaa
     1981 aaatgtttat ctaatgtact gagctcttct ctttattgta ttaactcttc gatttcagcg
     2041 gcgttgtcgt tcaggcatga aaaaaggagc cgaagctcct ttggtttcag aattcaaatt
     2101 gtcttgcccg caggcttttc agcattggca tggcccgctg gataacggaa cttgacatgt
     2161 cgagacgtgt tacctccctt agtagcgcgt ctctgttctt cgtcaccatg tagatagtct
     2221 caaacgcaat gtcatacagc ttgttcgtgt atgaggagtt cagctctttc attatggggt
     2281 acagctgctt gctaaggtcc tgggcttttt ccatccagag ttgcatgtag caaaggagga
     2341 tgatttcctc ggcggtaaat tgtggctgtg attctgcttg tactggctga atgttgcgaa
     2401 gtttcttttc gcactcgatg aagtagcggc ggatctggcg gcctttttcg tttcgttcaa
     2461 ccatcgcaag ctctttggct gtgtccaggg tgagatggta gtccttgcgg cggcggccta
     2521 gagttttcgc caaatttggc gaaaatagaa tgtagtctaa attttctacg aatccatact
     2581 cgtttagcct ggcccgtatc cagttagaaa aatctttatc tacctctaaa aacccatgca
     2641 aatcacgcgc gttcaccaga agcgtaggtt catttgagat tgtaccttca aacacaggga
     2701 tgagttgagt ggtcatgatg acctcctttg gcttttttcg agaaatgcca ccacaaaatg
     2761 tggtgccggg aggctcgaaa cggcccaaaa gataccgcgg acttattccc cttgcgggtg
     2821 ttgtattcgt cgccctcccg acatggatcg gggatgtgac cgcacattgt gccatcactg
     2881 aatgacaggc ataaaaaatc caacactgac ggggttggct taatccgctt ttgggaggtt
     2941 ttcgaggtct cggcgcggag tatagtcact attggatgct gtcgtcaaca gcgtggttac
     3001 aattcattcc aaatgcgata gttttttatt tgccatatta agctattgga gaccctgtat
     3061 ttatctgcaa gaactgaatt tggtatactc atttcattag ctcttataaa tagcacttgc
     3121 tctttagtta gttttgctcg cccatgtttt tcatgacgtg cttggcgttt tcgtttctgt
     3181 cgatcagcat tattatcaag gattgttcct attagtaaat gctttggatt acaacatgca
     3241 ggattatcgc aagaatgcct gacaaccatg cccgcaggaa tttctccaat aaaaattcgg
     3301 taaattaacc tatgcgcgcc agttttaata cgtttaccct ttgagccgat taaaagacgc
     3361 ccataaccaa aatcatcttt tggtaaaata ttttcaatgc aattgtcagg aaaattattt
     3421 aaaaggtttt tcatgagaag cccgatataa ataaaaaagc cgcaaagcta tcgggtgcgg
     3481 atgaccgctt atttgttcag tgcggtcagt atgcgatagc tcaggcggat ttgtcaatct
     3541 gcggaaatat tgatgaacga gtgacggtat atacccaacc cggacaatta acgtaaattc
     3601 acatcgtaaa cttgcgttaa ttgtctgaaa tatctactta aatcctatta aatttttggg
     3661 gcactattgg ggcaaaatgt ggttgtttgg ggcatatttg gggcaaaaaa gaacgtataa
     3721 ataacggtgt aaaatcgatt tcttcatggt caaaatgagg tgtaacagat tgaaaaatat
     3781 gtgctcttgg acgatcttcg gtaattctgt gtttttaatg catcatggtt tcaatatgtc
     3841 cgtacatgga ataataaagc accagaactt tagccatttc taaccactcc tcgtgttatc
     3901 tctattccgt agcgattcgc taccacttat ttaaagataa gacgtccttt tcagagtgca
     3961 aatttcacaa ccacttattt gatttataac aactttcaca agcacgtaat tttgtcgcaa
     4021 aatgacacat ttttatctca tcgcgttttt ttaatcataa gagcggctta tggataatta
     4081 ttggagatga tatctattct cgctaagaag ctgttgcagg atattaccaa acgcgggtct
     4141 gcccgcgtca gtttactaag cttagtccca cgtagcgaaa atatggctgc cgccatacgc
     4201 cgcgttaatt ctatgcaata tgatgtctat acccagacgg aggtcagtaa tggcaaacca
     4261 tcgaggcggt tccggcaatt ttgcagaaga ccgcgaaaga gcatcagaag caggtaaaaa
     4321 aggtggacag cacagcgggg gtaatttcaa aaatgacccg cagcgcgcat ctgaagcagg
     4381 taaaaaaggt ggcaagagca gtcacggtaa aagcgacaac tagccgggct aatcaatgac
     4441 gcatgcattt ttgtctgtag ctcgtcaaaa cgccatcacc gccggttacc ccggcggttt
     4501 tctgtttatg gctcctgatg aacaacttct ggcggtggaa cgtcaaccaa ccttctgctt
     4561 aacaacgcat tgagtaaaat cgcgccaaag gtggctgtac cgatccctcc caacgtaaaa
     4621 ccgcccagtg tgagagcaaa atcacccgca cccagcacta aggttactgc gaccataatc
     4681 aaattaccgt tctggcttaa atccacactg ttttgtaccc agatccgtgc gcctgcgacg
     4741 gcaatcagcc cgaacacaac aattgatgca ccaccaatca ccgcggccgg aatagtatgg
     4801 atcagcgcgc caaatttcgg cgaaaagccc aacagcatgg cgatgacggc agcggcaaca
     4861 aacaccagcg tcgagtagac ttttgtcacg gccatcacac caatattctc ggcgtaggtg
     4921 gttacgccgc taccgccgac agagccagaa agcatcgtcg ccagcccatc gcctacgaat
     4981 gcccgcccca tatacgggtc catattgcgt ccggtcatcc cggcgaccgc cttgagatga
     5041 cctaagtttt ccgccaccag aatcaccgcc acgggcgcaa tcagcatcat tgcctgacca
     5101 ttaaaagcag gagtggaaaa atgtggcaga ccgaaccagg cagcatggct gacgagagta
     5161 aaatcgacgg cttttcccag ccctaaaacg ttggtcatca cgccatacag cagacaggca
     5221 acaattaatc ctacgagaat caataatcgc tggatcatgc cacgggtaaa tacggccacc
     5281 agcccaatac acagcaccgt cattaccgcc atccagctat caaaggccga agccgataca
     5341 tttttcactg cgataggcgc taagttcagg ccaatcgcca tcactaccgc acccgtcaca
     5401 accggcggca ttagtcgttc aatccagcgc gtaccgattt tcatcaccac caggccaatg
     5461 acggtataaa ccagcccaca ggcgataatc ccgcccagcg cgatgctgat attcggatta
     5521 attccctgac cattaaagcc cgttgcggca atcaccacgc cgacaaaagc cgcgctggaa
     5581 ccgagataac tgggcacgcg cccgccggta ataaagaaaa acagtaacgt gccgatcccc
     5641 gacattaaaa tggaaagatt gggatccagc cccatcagaa tcggcattaa caccgtcgcg
     5701 ccaaacatcg ccaccgcgtg ttgaacgccc attactgccg tctgagcaaa cggcaatcgt
     5761 tcatccggcg cgaccacgcc gctctctgta gaggtcgatt ttaactgcca gtgaggaaaa
     5821 ccgaacattg ccatcagctg tctccttaag gaggttaaca aacagggcgc atcagcgcgt
     5881 gataactgcg atcgaaccac accagcccgt agggtgtggc gtgacgatga atcgcttcga
     5941 tggcgcaaaa caaaatgtcg tgggtgccga cgctcaccac ctggctgata cggcagtcaa
     6001 acgaaaccag tgcctcttcc agttgcgggc atccggtcac accagtctgc cagcgggcgg
     6061 cggcgaagcg gtgttccatg ggcgttttgc cgccaaaaag gtttgaaagc ggctcctgcc
     6121 cggcgctaag tgtatttaca cacagcgttc gattttcatt gaataccggc cagacggacg
     6181 ccccacgatt caggcacacc agtaatgtgg gcggcgtgtc ggtcacgctg cagacggcgc
     6241 tggcggtgaa cccggcgcgc ccggctggac cgtccgtagt gatgatattg accgccgcgc
     6301 ccatacagga catcgcatcg cgaaaggttt gttgatcgac aatgttcatt gtttgctcct
     6361 tacaacagcc cgcaggcttc ttcaaaggac agacgtggca ggcgcgcaaa aagtttgctg
     6421 gaatcgccat agccgatatt aatcagcaga ttgctcttca gcgtgctgcc cgcaaaaaag
     6481 gcgtcgtcaa cgtattgacg gtcaaagccc gacatcgggc cggtatccag ccccagcgca
     6541 cggcaggcga cgatcagata ggccgcctgc atggaactgt tgcgaaacgc tgtttcttcg
     6601 gcaagttgtg gactggaggt aaaccaactg cgggcatcgc cgtgaggaaa cagctgtggt
     6661 aaccgttcat aaaattcact gtcccaggcg acgatagcgg tgacgggcgc ggtcagggtt
     6721 ttttgcagat tgccgctgga aagcgccggg cgaagacgtt cttttccttc tgcagtgcgg
     6781 ataaacacga tccgtgccgg agaacagtta gcagatgttg gcccccattt catcagggca
     6841 taaatctccc gtaacgtctc atcgctgacg ggtgtctccc gccagccgtt gtgagtgcgg
     6901 gcatcggtga acagggtgct aagcgcacct gggctaacgg cttcgttcat agcaattcct
     6961 tacagggcgg cttcacggtg atgtaacagg ctggcaagcc cgttgagtaa cagagcatta
     7021 aacgtttcgg gatcggtcac gttgcaggcg tgtccgccat agcgcatcac cattttctgg
     7081 ctatcgggca gagcggcatg aagttcactg gaacatgctg atggcaccag cagatcatca
     7141 ctggcgcaga tgatttgcac cgggcagcgg atacgatccg catggcgact aaagtcagcg
     7201 cgtttgaggg cgttaagtcg acgcagtaaa ttatttttgc cctgaaaatg cgccagtgcc
     7261 agcgcgtctt ctgcctcaag gcgaggtgcg cgggccgcca tccagtcggc gggatagagg
     7321 aacaacggct gcgcttccac ccatgcctgc gcgccgccgc tatgtagtaa ctgttcgcga
     7381 acctgaaaac agcggcgcgt atgggcgttt attcgtagcc agccgttaac gctgaccagc
     7441 acagttaccg acgcgggata atccagcgcc agctgcattc ccaccagcgc accgagcgca
     7501 tggccgacca ccgcgtaacg ctcaatccct gcggctacca gcgcctgatg cagttccgct
     7561 gccatctggg cgatactgta atcttctgcc agcgtgtcgg gattattgcc ggtgccgcgc
     7621 tggtcgtaac agactacctg atactcctga tccagcaccg ccagttgcgg taaccagtaa
     7681 ctgccgctac cgccaagacc cgaaatcaac accactacgg gcgcatcagc ataaggggga
     7741 ggtgagagtg aaagtttcat cgcggcctca cttggcgata tgcgcaattg tggcgatttc
     7801 caccagcgcg tcaggtttta ccagtccgca ctgaatgcag aatcgcgccg gtttgtcacc
     7861 cgggaaaaac tcggcgtaga tttcgttaat cgcggcgtaa tttttccagt cggtaataaa
     7921 gatgctgttg aaggtcacat ccgccatcgt gccacccgcc gtctcgatga ccttgcggat
     7981 ggtttccaga acgtggcggg tttgcgcctt tgggtcatcg gcaaacagca cgttgttatg
     8041 ttgatcaaaa gccagcgtac cggagacata caccacgcca tcagccagcg tgccgggaac
     8101 gaagggggcc agcggtgcgc tgctgccagc aggaataatt acggattttg gcatcgttaa
     8161 actccttaag cgatacgagc aaaggacgtg gaagaaagcg cgtcgcagaa cgtttcgacg
     8221 tcgctgaccc agccaaaaaa ggtttcgata ttgaacaacg cggctttctg tgcaaattcc
     8281 ggccccgcct ggtgtgttgc gtcttccagc accacgccga aatactccag aaaaaagccg
     8341 tcgcgtagcg tcgattcgac gcagacgttg gtagcgatac cggtgaaaac cagatggcgt
     8401 attccacggc tgcgcaaaat gctgtccagc ggcgtattga agaaaccgct gtagcgcggc
     8461 ttcggcagca caatatcgcc aggctgcggc accagttcat ccaccagttg ataatcccag
     8521 gagcctttcg ccagcaattt cccctgcagc tgcggctgat tacgcatggt tttcagggcg
     8581 ttcgatttat gaaaattcgg tgagccgggg ccgccagcct cgacatactg ttcatcccag
     8641 ccattttgaa accagatgat cagcatccct gccgttcgcg ctgcggtcac ggcggtttga
     8701 atgttggcaa tgaccgggcg agtggttgag acatcaaacc cggcgagatc taagtagccg
     8761 cctggcgtgg cataggcatt ttgcatatcc accacgatca gcgcagtttg ctgcggatcg
     8821 aaggtaatgg cttccggtcg tgcggttaag gttgtcatca tgccacctcc tgagtcagcg
     8881 cagggagatg ggcgcggcac agcatcagtg gttgaatgcg ctcgccgaag gtttcgattc
     8941 ccgacagaaa atcatcgaag gtcaacagca cgccttcggc accaggcacg tttgcgactt
     9001 catctaacat gcgcgcgaca ctggcgtaag aaccgactaa cgtccccata ttgatgttta
     9061 ccgccgaagt gggatcggcc atctgacgga cgttggtgtc agtacctgag cgggtatctt
     9121 tctgactttg ttcagttagc cagcttaacg cctcttcatc cgcgcccgct ttgtagtgtt
     9181 cccattttgc acgagcggca tcgtcggttt cgtcggtaat caccataaac aacacataag
     9241 agccaacgtc gcgtccggtt tgctctgcgg cctgtttcat ccgcgcagcg gtcggggcga
     9301 aagccgtggg tgtatttacg cctttgccga aacagaagtt aaaatcggca taccgggcgg
     9361 agaacgccat gccagcgtcg ctttgcccgg cgcatatcac tttcatgggg acactcggtt
     9421 gcggactgac gcgacaatca tccatagtga aaaaatcgcc tttaaaatcg cttttccccg
     9481 agccccacag gtcgcgcagc acctgaacat attcggtgag atagtcgtaa cgacgggaga
     9541 aatagtcatc gccaggccag atccccatct gctcatactc gggcttctgc cagccagtca
     9601 cgaggttgac gccaaaacgc ccgccagaga tggagtcgat ggttgcggcc atacgggcga
     9661 cgattgccgg aggtaacgtt aaggtggcag ctgtcgcgta tatctgaatg cgtgaggtga
     9721 cggctgccag ccccgccatc aaggtgaacg actcaaggtt gtgatcccag aactcagttt
     9781 tgccgccaaa gccacgcagt ttgatcatcg acagggcgaa atcgaaatgg tagtgctccg
     9841 ctttttgcac aatggcttta ttcagttcaa atgtcggcat gtactgcggc gcgtgggtcg
     9901 aaatgagcca gccgttgttg ccaataggta cgaatacgcc aattttcatc atcaacctct
     9961 cttcgtctcg taaagtgaaa gtcagacggg gcgctgcatc ctgcatatcc ttttcagccg
    10021 cgtaatggct tgtttgcaaa gcggatgcca gtttttaaaa agtgaatgtt attaatgtgt
    10081 taatgttaag ttgtctaaaa tgcctaaaaa aaagtggact aaacggtcaa aacagttgca
    10141 cataaaacat gcattcgtgc gcgatgagag tgcagaaggt cgaggccggg cgggggtttt
    10201 gctatcctgt tgccaatcaa caagagggga gagcgcatga cgcaaggcgc agtgaaaaca
    10261 acgggtaagc gttcgcgcgc agtaagcgcg aagaaaaaag cgattcttag cgcagcactg
    10321 gacacttttt cacaattcgg ttttcacggc acaaggctgg agcagatcgc ggagttggcg
    10381 ggtgtttcaa aaaccaatct gctgtattac tttccgtcaa aagaggcgct gtatatagcc
    10441 gtactgcggc agattctcga tatctggctg gcaccgttaa aagcgtttcg agaagatttc
    10501 gccccgctgg cggcgatcaa agaatacatc cgtctgaagc tggaagtttc ccgcgattat
    10561 ccgcaggctt cgcgcctgtt ctgtatggag atgctggcag gtgcaccgct gttaatggat
    10621 gaactgacgg gcgatttgaa ggcattaatt gatgagaaat cggcgctcat tgccggttgg
    10681 gttaaaagcg gcaaactcgc gccgattgac ccgcagcatt tgatttttat gatttgggct
    10741 tccactcaac attacgccga ttttgcccct caggtggagg cggtgacagg cgcgacgttg
    10801 cgcgatgagg tgtttttcaa tcaaacggtt gaaaacgtgc agcggattat tattgagggg
    10861 attcgaccac gttaaagatg ccggaggagg ttgtaacatc ctccggctac ctgtttaacc
    10921 gatcgtcatt aaactggcgt taccgccagc agcagcggta ttcacactca gcgaacgctc
    10981 gatatacagc cgttccagaa ggatattgct ttcgccacgg gcaaaaccct gcaccgaaac
    11041 aattgcacca tcccgtgcgg caacggcttc acacaatgcg cgaagctgat ccgaatcacc
    11101 gtggaagatc accgcatcaa atggttgagc ggttatattt tccgctttcg ccagttgaat
    11161 acgttcgctg actgccgatg gcaatgcctt cactaactga cgatgcagcg catcatccgg
    11221 ccacagcacc tggctaccca ccgccagcac ggcggcgagc tgagtcagcg catcctgttc
    11281 atcatccgca atacacaaca cgcgctcacg cggcagcagc gtccaggtgt tgcgttcacc
    11341 cgtcggccct ggcaacaatc gttgtgttcc tgcctgcgcc aactcgccat attgcgtaca
    11401 taacgcctgc aattctggac ggtttgccgc ccattcccgc agtgcattta gcggctgagt
    11461 caatgcggct ttcaactgcg catcgaccgg atactccgca tcctgacgcg cgagcgtcac
    11521 cgccagcgca ctttccgggc gattcgccag cagacggtag agatagagcg gaccgcctgc
    11581 tttcggcccg gtaccggaca acccttcgcc gccgaacggt tgcaccccaa ccactgcgcc
    11641 caccatatta cggttaacgt acagattacc gacatgcgcc gagccagtga cctgggcgat
    11701 ggtttcatca atgcgcgtat ggacgccaag cgtcagacca taaccggaag cgttaatctg
    11761 ctcgatcagc gccggcagct ggttacggtt gtaacgcacc acatgcaaca ccggaccaaa
    11821 gacctctttt tgtaattcgg caaagtcatc cagttcgatc agcgtcggag cgacaaaggt
    11881 gccgctttgc cattcacggg catcttcgct gttttcccgc accgcctgga acacctgacg
    11941 gcctttacta cgcatggtct gaatatgacg ctcgatattg gctttcgctt cgctatcaat
    12001 caccggaccg atatcggtgg tcaggcgacc cggattaccc atccggcatt cggccattgc
    12061 gccgcgcagc attttcagcg tgtggtcggc aatctcatct tgcaggcaca gcacgcgcag
    12121 tgccgaacaa cgctgacctg cactgtcgaa cgccgaggcc agcacatcta ttacgacttg
    12181 ttcggtcagt gctgaagaat cgacaatcat cgcgttcatg ccgccggttt cagcgatgag
    12241 cggaataggg cgaccctgag cgtccaggcg gctggcgata ttgcgctgca gtaacgtagc
    12301 gacttcggtt gaaccggtaa acatcatccc gcgcacgcga tcatcacccg tcagttgcgc
    12361 gcccacggtt tcaccccgac cgggcagcaa ttgcaccacg cctggcggta cacccgcttc
    12421 cagcaaaatg gcgatccctt gcgcggcaat cagcggcgtt tgttctgccg gttttgccag
    12481 cacgctgtta cctgccgcca gtgcggcagc gatctgtccg gtgaaaatcg ccagcgggaa
    12541 gttccacgga ctgatacata ccacaggccc taatggacgg tgggtttcgt tagcgaaatc
    12601 atcccgcacc tgtccggcat agtagtggag aaaatcgacc gcttcgcgca cttcggcaat
    12661 ggcgttactg aaggtttttc cggcttcacg caccagaata ccaatcagtt gctgcatctg
    12721 gctttccatt agcacggcag cgcgatgcaa aattgccgcg cgttcagccg gaggcgtggc
    12781 aaaccagatt ggcgcgttat taaccgcact ttccagcgcc tgttctactt cacgcggcgt
    12841 ggcttcacgc acatagccca caatatcttt cggttccgca gggttaataa cgggcgacat
    12901 ctcaccagcc gcgaccgatt gttccagcat tggcaaggac tgccattttt gcagtgcact
    12961 attgagcagg gctgaagaga gcgaggccag acggtgttcg ttagcgagat ccagccctgc
    13021 cgagttgtcg cgcccgtgac cgtaaagatc gcgcggcagg ggaattttcg gatgcggtaa
    13081 tccagtttgc ccttcctgtt gcgccagttt ttctacagca gtgactggat cggcgaccag
    13141 ttcatccagt ggcaaagagg tgtcggcaat acggttaaca aacgaggtgt tagcaccgtt
    13201 ttccagcagg cggcgcacca gatacgccag cagcgtttca tgtgtaccga ccggagcata
    13261 aatacgacac ggacggttaa gtttgccgtc ggcaactttc ccggtgacct gctcatacag
    13321 tggttcgccc ataccatgca ggcactggaa ctcgtactga cccgggtagt agttttgacc
    13381 cgccagttga taaattgccg ccagcgtatg ggcgttgtgc gtcgcgaact gcgggtagat
    13441 taaattcggc accgccagca gttttttcgc acaggcaaga taagaaacgt cggtatacac
    13501 cttgcgggta taaaccggat aaccttcaag gccgtccatc tgtgcacgct tgatttcact
    13561 atcccagtac gcgcctttca ccaggcgaat catcagacgg cgacggctgc gggtggcgag
    13621 atcaatcagg taatcgatca ccaacgggca gcgtttttga taagcctgaa taacaaaacc
    13681 gatgccgttc cagcctgcca gttccggctc gaaacagagt ttttccagca gatcgaggga
    13741 gatctccaga cgatcggcct cttcggcatc gatgttaata ccaatatcgt actgacgcgc
    13801 cagcagggtg agtgatttca gacgcgggta aagctcttcc attacccggt catactgggc
    13861 gcggctgtaa cgcggatgca gcgccgacag cttgattgaa atgcccggac cttcatagat
    13921 accacgaccg ttagacgctt taccaatggc gtgaatcgcc tgctgatagg aaaccatata
    13981 cgcctgtgcg tctgcggcag tcagtgcagc ttcgcccagc atatcgtaag agtaacggaa
    14041 acctttctct tccagtttgc gggcattggc taacgcttcc gcgatggttt cgcccgtgac
    14101 gaactgctcg cccatcaggc gcatcgccat atccacacct ttgcggatca gcggttcacc
    14161 gctcttaccg ataatgcggt tcagcgagcg ggagaggctg gcttcgttat gggtggaaac
    14221 cagtttgccg gtaaacagca gcccccaggt agcggcatta acaaacaggg acgggctacg
    14281 accaatgtgt gactgccagt taccgttact gattttgtcg cgaattaacg cgtcacgggt
    14341 ggctttgtcg ggaatacgca acaacgcttc cgccagacac atcagcgcca cgccttcctg
    14401 cgatgacaac gaaaactcct gcaataaccc ctgaaccata cctgcgcgac cactggcgtt
    14461 tttttgatta cgcagtttat cggccagttg atacgccagt ttgtgcgcct gttcagctac
    14521 tggctgcggc aggcgagctt gttccagcag catagaaacc gcttcggttt ccgggcggcg
    14581 ataggcggcg gtgatggcgg cgcgggaaac cgactggggc aatatttgct cggcaaagtc
    14641 gaggaaaggc tggtgtggtt cctctgccgg agtcggtgct tcatcgctct cattggccgc
    14701 gccagaaagc agcgctggta gctccgggag agtatcgctg ttttccagtt gttcaagata
    14761 agaaaaaatc gcctgtttaa ttaaccagtg tggtgtgcga tcgatacgtg tcgcggcaga
    14821 cttaatacgc tcacgcgtcg cgtcgtccag cttaaccccc atggtggtgg ttcccatgcc
    14881 attactcctg ttgttctgaa aggtgcaact taacgttatc gtgaaatatc catgatgttg
    14941 caactttgtg caaccgtgtt aaatgtgacc tgcgtagcaa gcttaaaaat gaatgaaatg
    15001 ttaataaacg aaatcgatat gacagggatt aaaaaaataa ctcagacttt ttctctgcgg
    15061 cagttaacat ttttgaaagg tgcaaccgca aaaaatgtga gagagtgcaa cctgatgaaa
    15121 aatagtgtcg ctgagcacta aaatttaatg taaatggtgt gttaaatcga ttgtgaataa
    15181 ccagcgcttc cggcaggata cggtcgccct ggtaaaacat aaactctgtt accccgttcc
    15241 ggtggcagat ataacggcaa gtttcgacat tgccgataat aattttttgg agactttaga
    15301 tggctattag cacaccgatg ttggtgacat tttgtgtcta tatctttggc atgatattga
    15361 ttgggtttat cgcctggcga tcaacgaaaa acttcgacga ctatattctg ggcggtcgta
    15421 gtcttgggcc attcgtgacg gcattatcgg cgggtgcgtc ggatatgagc ggctggctgt
    15481 taatggggtt gccaggcgct gtttttcttt ccgggatttc cgaaagctgg atcgccattg
    15541 gtctgacatt aggcgcgtgg atcaactgga aactggtggc cgggcggttg cgtgtgcata
    15601 ccgaatataa caataacgcc ttaacattgc cggattattt caccgggcgc tttgaagata
    15661 aaagccgcat tttgcgcatt atctccgcgc tggttatttt gctgttcttc accatttatt
    15721 gcgcttcagg cattgtggca ggcgcgcgtc tgtttgaaag tacctttggc atgagctacg
    15781 aaacggctct gtgggcgggg gctgcggcga cgatccttta cacctttatt ggcggtttcc
    15841 tcgcggtgag ctggactgac actgtacagg ccagcctgat gatttttgcc ctgatcctga
    15901 cgccagttat cgtcattatc agtgtcggtg gctttggtga ctcgctggaa gtaatcaaac
    15961 aaaagagcat tgaaaacgtg gatatgctca aagggttgaa ttttgtcgcc attatctcgc
    16021 tgatgggttg ggggctgggt tacttcgggc agccgcacat cctggcgcgt tttatggcgg
    16081 cagattctca ccacagcatt gtccatgcgc gtcgtattag tatgacctgg atgatcctct
    16141 gcctggcagg ggcggtggct gtcggcttct ttggtatcgc ttactttaac gagcacccgg
    16201 cggtagcagg tgcggtaaac cagaacgccg agcgcgtgtt tattgaactg gcacaaattc
    16261 tgtttaaccc gtggattgcc gggattttgc tgtcggcgat tctggcggcg gtaatgtcaa
    16321 cgttaagttg ccagctgctg gtatgttcca gtgcgattac cgaagattta tacaaagcgt
    16381 ttctgcgtaa acaggccagc cagaaagagc tggtgtgggt agggcgagtg atggtgctgg
    16441 tggtggcgct ggtggcgatt gcgctggcgg caaacccgga aaaccgcgtg ctgggcttag
    16501 tgagctacgc gtgggcaggc tttggcgcag cgtttggtcc ggtggtgctg ttctcggtga
    16561 tgtggtcacg catgacgcgc aacggtgcgc tggcggggat gatcatcggt gcgctgacgg
    16621 ttatcgtctg gaaacagttc ggctggctgg gtctgtacga aattattccg ggctttatct
    16681 tcggcagtat cgggattgta gtgtttagtt tgctgggtaa agcgccgtca gcggcgatgc
    16741 aaaaacgctt tgccgaggcc gatgcgcact atcattcggc tccgccgtca cggttgcagg
    16801 aagggtaagg gaaggcgttt taccggaaat ttcacgtcat aacaggaagc aggcagaggg
    16861 caatcagtga ttgccctctg cgttttttct caaaatattt ttacatcttc tcatcattat
    16921 cactacattc cacttgtatt tttcttcatc gtaatgataa tcgctatcac tgcgatttac
    16981 ttttctttgc atagattgac tcagaaaaac gtttaagggt gggtggcatg tttgttccgt
    17041 ttctcattat gttgcgcgaa ggacttgaag ccgcgctgat tgtcagtttg attgccagct
    17101 atcttaagcg tacccagcga ggccgatgga ttggtgtaat gtggattggc gtgttgcttg
    17161 ccgctgcgtt gtgcctgggc ttgggtatct tcattaacga aaccaccggc gaatttccgc
    17221 aaaaagaaca ggaactgttt gaaggcattg tggcggtgat cgccgtagtg atccttacct
    17281 ggatggtttt ctggatgcgc aaagtgtcgc gcaacgtcaa agtacaactg gagcaggcgg
    17341 tcgatagcgc attgcagcgt ggaaatcatc atggctgggc gctggtgatg atggtctttt
    17401 ttgccgttgc aagggaaggg ctggagtcgg tctttttcct gctggcggca tttcaacaag
    17461 atgtcgggat ctggccgccg ctgggtgcaa tgcttggtct tgctacagcc gtggtgcttg
    17521 gcttcctgct ctactggggc ggtattcgcc tcaatcttgg tgcatttttt aaatggacca
    17581 gtctgtttat tctcttcgtc gccgcagggc tggcagctgg tgccattcgc gcatttcatg
    17641 aagccggatt gtggaaccac tttcaggaaa tcgccttcga tatgagcgcg gtgctctcaa
    17701 ctcactcgct gtttggcaca ctgatggaag ggatttttgg ttatcaggaa gcaccgagcg
    17761 tcagcgaagt cgccgtctgg tttatttatc tcatcccggc gctggtggca tttgttctgc
    17821 caccacgcgc aggggcgact gcgtcccgct ccgtgtagca aatacgacgc aaactcttgc
    17881 ttagttacaa catactttaa agggatagtc tcgtcatgac cattaacttc cgccgtaacg
    17941 cattgcagtt gagcgtggct gcgctgttct cttctgcttt tatggctaac gccgctgata
    18001 ttccgcaagt caaagtgacc gtgacggata agcagtgcga gccgatgacc attacggtta
    18061 acgccgggaa aacacagttc attattcaga accacagcca gaaggcgctg gagtgggaga
    18121 tcctcaaagg cgtgatggtg gtggaagagc gggaaaatat cgcccctggt tttagccaga
    18181 aaatgacggc gaatttacag cctggcgaat acgatatgac ctgcggtctg ctgactaacc
    18241 cgaaagggaa gttgatcgtc aaaggtgaag caacggcgga tgcggcgcaa agtgatgcgc
    18301 tgttaagtct tggtggtgca attactgcat ataaagcgta tgtcatggcg gaaaccacgc
    18361 aactggtgac cgacaccaaa gcctttaccg acgctattaa agcaggcgat atcgaaaaag
    18421 cgaaagcact gtatgcgccg acgcgccagc actatgagcg cattgaaccg attgctgaac
    18481 tgttctccga tctggatggc agcattgacg cccgtgaaga tgattacgag caaaaagccg
    18541 ccgatccgaa attcaccggt ttccaccgtc tggaaaaagc attgtttggc gataacacca
    18601 ccaaaggaat ggataagtat gctgaccagc tttataccga tgtggtcgat ttgcaaaaac
    18661 gcatcagtga actggctttc cctccttcaa aagtggtcgg cggtgcagcc ggactgattg
    18721 aggaagtggc agccagcaaa atcagcggtg aagaagatcg ctacagccac accgatctgt
    18781 gggatttcca ggctaacgtt gaaggctcgc agaaaattgt cgatctgctg cgtccacaac
    18841 tacaaaaagc gaatccggaa ctgttggcaa aagtcgatgc caacttcaaa aaggtcgata
    18901 ccattctggc gaaataccgt actaaagacg gttttgaaaa ttacgacaaa ctgaccgatg
    18961 ctgaccggaa tgcactgaaa ggaccgatta ctgcgctggc ggaagatctg gcgcaacttc
    19021 gcggtgtgct ggggctggac taagcgttat gcagtatgaa gatgaaaacg gcgtgaatga
    19081 accgtcacgc cgacgtttac tgaaagggat tggtgcgctg gcgctggcgg gaagttgtcc
    19141 ggtcgctcat gcacaaaaaa cgcaaagtgc gccgggtacg ctttcaccgg atgcacgcaa
    19201 tgagaaacag ccgttttatg gtgagcatca ggcagggatc ctgacaccac aacaggctgc
    19261 aatgatgctg gtggcgtttg atgtgcttgc cagcgataaa gccgatcttg aacggttgtt
    19321 tcgcttgttg actcagcgtt ttgcttttct gactcagggc ggagttgcac cagaaacgcc
    19381 aaatccgcgc ctgccaccac tcgattccgg cattcttggt ggctacattg cgcccgataa
    19441 tctcaccatc acgttatcgg tgggtcactc attgtttgat gagcgctttg gccttgcgcc
    19501 acagatgcca aaaaagttgc agaagatgac gcgtttccct aacgactcgc tggatgcggc
    19561 gttatgtcat ggtgatgtgt tgctacaaat ttgcgccaac acccaggaca cggtgatcca
    19621 tgcgctgcgc gatatcatca aacacacgcc ggatttgcta agcgtgcgct ggaagcggga
    19681 agggtttatt tccgaccacg cagcgcgtag taaaggcaaa gagacgccga taaatttgct
    19741 gggctttaaa gacggcaccg ccaatcccga tagccagaat gtgaagttga tgcaaaaagt
    19801 ggtgtgggtg acggcagatc agcaggagcc tgcgtggaca atcggtggca gctatcaggc
    19861 agtgcgcttg attcagtttc gagtggaatt ttgggacaga acgccgctga aagagcagca
    19921 gacgatattt ggtcgtgata agcaaaccgg tgcgccgctg ggcatgctgc atgagcatga
    19981 tgttcccgat tacgctagcg atccggaagg gaaggtgatt gcgctggaca gccatatccg
    20041 gctggcgaat ccccgcacgg cggagagcga gtccagcctg atgctgcgtc gtggctacag
    20101 ttattcgttg ggcgtcacca attccgggca actcgatatg gagctactgt ttgtctgcta
    20161 ccaacacgat ctggaaaagg gcttcctgac agtacaaaaa aggttgaatg gcgaggcgct
    20221 ggaggaatat gtcaaaccta tcggtggcgg ctatttcttt gcgctgccgg gtgtgaagga
    20281 cgcgaacgat tatctcggaa gcgcattatt gcgggtttaa tgtttttagg cggataaggc
    20341 gtttacgccg caccaggcgt tcgtgcgctg atgcgacgct tgcgcgtctt atcatgccta
    20401 caatcggtgc gggtttggta ggctggatca ggcgttcacg ccgcatccgg cgttcgtgcg
    20461 ctgatgcctg atgcgaatcc tgctgaaagc acacagcttt tttcatcact gtcatcactc
    20521 tgtcatcttt ccagtagaaa ctaatgtcac tgaaatggtg ttttatagtt aaatataagt
    20581 gaatatattg ttgcaatgaa tgcgagatct gttgtactta ttaagtagca gcggaagttc
    20641 ccggcagtga tagtcagtca ctatggagat cgcggatggt aacgtcctgt tctggacaat
    20701 gttttgttaa gcaaggcccc accactcgcg gagatttctc ctccggtagc aacttcgtca
    20761 ctctgtattt ttctcacttc ctctctttac tcgtgttacc gacgccgcaa acggtgtgcg
    20821 tagccgtttt tcggcatttt atccaaaagc aaactatggc ttacaaggaa gccaaccctc
    20881 tgatgttcgt gcgcataatc gcgctgccaa cggcgcgtgt gatgaataca aacaactcaa
    20941 ggtgctctcc atgggaagac aaaaagcagt gatcaaagct cgtcgcgagg caaaacgtgt
    21001 gctgagacgg gattcgcgca gccataagca gcgtgaagaa gaatcggtca cctcgcttgt
    21061 gcagatgagc ggcgtagaag ccattggtat ggcccgcgac agtcgcgata cctcgcccat
    21121 cctcgcgcga aatgaagcgc aattgcacta tctgaaggct attgagagta agcagctgat
    21181 atttgccacg ggcgaagccg ggtgcggaaa aacctggatc agcgcagcaa aagcggcaga
    21241 ggccctgata cataaggatg tcgacaggat tatcgttacc cgtccagttc tgcaagccga
    21301 tgaagatctt ggtttcttac ctggtgatat cgcagaaaag tttgctccct attttcgccc
    21361 ggtctacgac gtgctggtcc gtcgcttagg ggcttccttt atgcagtact gcctgcgacc
    21421 ggaaataggg aaggtggaaa ttgcgccgtt cgcctatatg cgtggacgta cctttgaaaa
    21481 tgcagtcgtc attcttgacg aggcgcagaa tgtgactgcc gcgcaaatga aaatgttttt
    21541 aacccgcctc ggggagaacg tgacggttat cgtcaacggt gatatcacgc aatgcgattt
    21601 gcctcgcggc gtgcgctccg gattaagtga cgcgctggaa cgtttcgaag aggatgaaat
    21661 ggtcgggatc gtccgattcg gtaaagagga ttgcgtacgt tcggcacttt gccaacgtac
    21721 gctacatgca tacagttaag tgtattatcg gtgcagagcc cgggcgaacc
//
LOCUS       DAZFFE010000082        21448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFFE010000082 DAZFFE010000000
VERSION     DAZFFE010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.082"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..113)
                     /locus_tag="ACHJ69_003315"
                     /pseudo
     CDS             complement(1..113)
                     /locus_tag="ACHJ69_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            114..270
                     /locus_tag="ACHJ69_003316"
                     /pseudo
     CDS             114..270
                     /locus_tag="ACHJ69_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
     gene            complement(363..1478)
                     /gene="rnd"
                     /locus_tag="ACHJ69_003317"
     CDS             complement(363..1478)
                     /gene="rnd"
                     /locus_tag="ACHJ69_003317"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO5016239.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQALPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(1560..3245)
                     /gene="fadD"
                     /locus_tag="ACHJ69_003318"
     CDS             complement(1560..3245)
                     /gene="fadD"
                     /locus_tag="ACHJ69_003318"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO5016240.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(3450..4031)
                     /gene="yeaY"
                     /locus_tag="ACHJ69_003319"
     CDS             complement(3450..4031)
                     /gene="yeaY"
                     /locus_tag="ACHJ69_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO5016241.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQNLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(4071..4766)
                     /gene="tsaB"
                     /locus_tag="ACHJ69_003320"
     CDS             complement(4071..4766)
                     /gene="tsaB"
                     /locus_tag="ACHJ69_003320"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO5016242.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLLDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(4824..6734)
                     /gene="yoaA"
                     /locus_tag="ACHJ69_003321"
     CDS             complement(4824..6734)
                     /gene="yoaA"
                     /locus_tag="ACHJ69_003321"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO5016243.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIELAESLLLPSPFDYSRQALLYVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            6866..7210
                     /gene="yoaB"
                     /locus_tag="ACHJ69_003322"
     CDS             6866..7210
                     /gene="yoaB"
                     /locus_tag="ACHJ69_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO5016244.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFDQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            7632..7931
                     /gene="yoaC"
                     /locus_tag="ACHJ69_003323"
     CDS             7632..7931
                     /gene="yoaC"
                     /locus_tag="ACHJ69_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO5016245.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITTRADLEGWNPGFTEKMVGWAKKMESGERIVIKNPEYFSTYMQEELKALV"
     gene            complement(8051..8230)
                     /gene="yoaH"
                     /locus_tag="ACHJ69_003324"
     CDS             complement(8051..8230)
                     /gene="yoaH"
                     /locus_tag="ACHJ69_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO5016246.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIMARFEDEDE"
     gene            8304..9665
                     /gene="pabB"
                     /locus_tag="ACHJ69_003325"
     CDS             8304..9665
                     /gene="pabB"
                     /locus_tag="ACHJ69_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO5016247.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALVVDHQRKTVSLLSHNDV
                     NARRTWLENQQFSPQEDFALTSDWQSNMTRELYGEKFRKVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQAAILSLSPERFILCDNGEIQTRPIKGT
                     LPRLPDLQEDSKQAEKLANSTKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPHRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            9669..10247
                     /gene="nudL"
                     /locus_tag="ACHJ69_003326"
     CDS             9669..10247
                     /gene="nudL"
                     /locus_tag="ACHJ69_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO5016248.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            10431..11795
                     /gene="sdaA"
                     /locus_tag="ACHJ69_003327"
     CDS             10431..11795
                     /gene="sdaA"
                     /locus_tag="ACHJ69_003327"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO5016249.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGP
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            11926..13524
                     /gene="pdeD"
                     /locus_tag="ACHJ69_003328"
     CDS             11926..13524
                     /gene="pdeD"
                     /locus_tag="ACHJ69_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO5016250.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDSRHLLYGIGLVDSLPQPEHNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRSHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(13528..15084)
                     /gene="yoaE"
                     /locus_tag="ACHJ69_003329"
     CDS             complement(13528..15084)
                     /gene="yoaE"
                     /locus_tag="ACHJ69_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO5016251.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(15072..15230)
                     /gene="yoaL"
                     /locus_tag="ACHJ69_003330"
     CDS             complement(15072..15230)
                     /gene="yoaL"
                     /locus_tag="ACHJ69_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO5016252.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            15547..16518
                     /gene="manX"
                     /locus_tag="ACHJ69_003331"
     CDS             15547..16518
                     /gene="manX"
                     /locus_tag="ACHJ69_003331"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO5016253.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVITGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            16581..17381
                     /gene="manY"
                     /locus_tag="ACHJ69_003332"
     CDS             16581..17381
                     /gene="manY"
                     /locus_tag="ACHJ69_003332"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO5016254.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            17394..18245
                     /gene="manZ"
                     /locus_tag="ACHJ69_003333"
     CDS             17394..18245
                     /gene="manZ"
                     /locus_tag="ACHJ69_003333"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO5016255.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            18300..18758
                     /gene="yobD"
                     /locus_tag="ACHJ69_003334"
     CDS             18300..18758
                     /gene="yobD"
                     /locus_tag="ACHJ69_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO5016256.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMHRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            19187..19753
                     /gene="mntP"
                     /locus_tag="ACHJ69_003335"
     CDS             19187..19753
                     /gene="mntP"
                     /locus_tag="ACHJ69_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO5016257.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(19750..20559)
                     /gene="rlmA"
                     /locus_tag="ACHJ69_003336"
     CDS             complement(19750..20559)
                     /gene="rlmA"
                     /locus_tag="ACHJ69_003336"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO5016258.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPLRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVGQLRERLDAKAAAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLKGFTLQQSDELCY
                     LMRLRGDEAVALLQMTPFAWRAKPEVWQALAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(20725..20934)
                     /gene="cspE"
                     /locus_tag="ACHJ69_003337"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(20725..20934)
                     /gene="cspE"
                     /locus_tag="ACHJ69_003337"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO5016259.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(20947..21090)
                     /gene="yobF"
                     /locus_tag="ACHJ69_003338"
     CDS             complement(20947..21090)
                     /gene="yobF"
                     /locus_tag="ACHJ69_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO5016260.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
BASE COUNT         5279 a         5516 c         5281 g         5372 t
ORIGIN      
        1 aattctaccg ttatccgcac tttcgctgac gtcgacagct ttgaaacata aacggcccag
       61 acattcactg gctggtaata ttccggtagc acttgtacta aatgaccact ggcgggaaac
      121 agcctggcga catcgatttt tacgtggtcc ggaaaaacac cgaaggcgaa tattcctcgc
      181 caggtggtag agtaaatgga aggaacagag aatgaagtcg tcattcagga atcggtattt
      241 actcgtcgtg gtgtcgagcg cattttgcgt taagctcaaa taagttcatt tgaaccacct
      301 gctgttggat ttgtagtttg ccgaagccgg actgagcgcg cccagtccgg cttcggaaga
      361 ttttactgtg gatattcctg caataaattg tgtaatgctt ccgccatcaa ctcaccacgc
      421 cagccggaaa tcagctccgg caaattgttc tgcggtttca gtttccagtg ccagttcagc
      481 agttggttga tttgccgacg cgacgccagc aattcggcgc tgatcttgtg cgtttcgctg
      541 acgtcagtaa tcagcgactt aatcgcttta aacgctttac gataacccgg catgtccatc
      601 aggttaagca tcggctgcgg taaggcctct tctggcaatg cctgcgcttt ttccaccagc
      661 gccagcaacg ttttaccgtg gaagcggatt tcgctaccgg ataagcccag gctgtccagt
      721 tcgcccagac ttcccggcat ataacgcgct accgaccaca aatgctcttc acgcacgaca
      781 aagttcaccg ccagatcgcg ctctcgcgcc ttgcgcaatc gccagtcggc taacagttgc
      841 agacaggcca gttggcgtgt gcgtaattgc caggcattgg tgatatcacg ccaggcgtct
      901 tccggcgcaa cgacttcctg acgacgcatt tgcatcaggc ggcattcatc cagcgccgca
      961 ggtagccagc cggaggcctc cgtttctacc ataagctttg cggtgatcgg taacagatac
     1021 cagacatccg ccgctgcgta ttcacactga cgttcggtca gcggtctggc cagccagtcg
     1081 gtgcgcgatt cactcttgtc cagcgtaacg ccggaatact cttccaccat ggaagcgaaa
     1141 ccccatgaca tcgggcgtcc gcagaaggca gcaaggattt gcgtgtcaat caagggttgt
     1201 ggtaattcgc caaagacatt gaggaacact tccagatctt cactgcctgc atgaagaaat
     1261 tttgtgatgg acggatcgcg caggatcgct ttcagcggtg accagtcggt gatcccgaga
     1321 ggatcaatta gcgccagatg ctcaccatca aaaagttgaa ttaaacccag ctgcgggtaa
     1381 taagtgcgcg tacgaacaaa ttcagtatcc agggctatcg ccggaaaggc acggacggct
     1441 tcacacaaag aagccagcgc atcgtccgtg gtaatcattt ggtaattcaa attgtattct
     1501 ctttagtggg cgtcaaaaaa aacgccggat taaccggcgt ctgacgactg acttaacgct
     1561 caggctttat tgtccacttt gccgcgcgct tcgtcacgta attctcgtcg caaaattttt
     1621 ccgacgttag atttcggtaa ctcatcacga aactccacca gcttcggtac tttgtagccc
     1681 gtgagctgac ggcggcaaaa agtcaccagt gactcttcgg taagcgatgg atcttttttc
     1741 actacgaaga ttttcaccgc ttcaccactg gagccagaag gtacgccaac agccgcgact
     1801 tcctgtacgc caggatgctg catgacgaca tcttcaatct cgttgggata gacgttaaaa
     1861 ccggaaacca gaatcatgtc ttttttgcga tcgacaatgc gcaggaatcc ttcttcatcc
     1921 atcaccgcga tgtcgccggt gtgtaaccag ccatttttga tgatttcatc ggtagcatcc
     1981 ggacgctgcc agtaacccag catcacctgc ggtcctttga cacaaagctc acctggttga
     2041 cccggtggta cttcattatc atcatcatcc accagtttgg cttccgtcga cggcaccggt
     2101 aaaccgatgc taccactatg ataatcaata tcatacgggt taacgctgac cagcggcgca
     2161 cactcggtca ggccataacc ttccagcaga tactgtccag tcagtttcac ccaacgttct
     2221 gccaccactt gctgaacagg catccctccg cctgcggaaa gatgcagact ggagaaatcc
     2281 agctgctgga actctttatt gttcagcaac gcattgaaca aggtgttaac gccagtgata
     2341 gcggtaaacg gatatttcgc taactctttt accaaccctg gaatatcgcg cgggttagtg
     2401 ataagcaggt tctgcccacc cagttcgata aacagcaggc agttaatggt cagggcaaaa
     2461 atgtgataca gcggcagcgc cgtcaccacc agctctttgc ccggatgcaa cagcggacca
     2521 taggtcgcgt taacctgttc caggttcgcc agcatattgc ggtgagtcag catcgcgcct
     2581 ttcgccacac cagtggtgcc gccggtgtat tgcaggaaag ctaaatcttc cggcaccagt
     2641 tctggtttga catactgcat ccggtagccg ttgtgcaggg cgctacgaaa tgaaatggca
     2701 tctggcagat ggtatttcgg caccagacgc ttgatgtatt taacaacgaa attgactacc
     2761 gtgccttttg ccgtagatag ctgatcgccc atacgggtca gaataacgtg ctgaacggcg
     2821 gttttatcaa ccactttttc cagagtgtgg gcaaagttag acacgataac aatcgccgat
     2881 gcgccgctat cgttaagctg atgctcaagc tcacgcgggg tatacaacgg gttaacgttt
     2941 acgacgatca tcccggcacg caaaatgcca aacagcgcca ccggatattg caataagtta
     3001 ggcatcatca acgcaacgcg atcgcctttc ttcagcccca acccttgttg caaataagcg
     3061 gcaaacgcgc gactgcgttc ttccagtttg cggaaggtca tcacctcccc catattcaca
     3121 aacgcgggtt gatcggcgta gcgcgcgacc gactgctcaa acatatctac cagagattga
     3181 taacggtcag ggttgatctc cgtcggaacg tccgcgggat aacggttaag ccaaaccttc
     3241 ttcaattctt cacctctaaa atgcgtgttc gtcgtcatcg caaccccaag taacaaacat
     3301 atcgttaaca taatattaac tcatcatacc agcttgataa ttacccaacg aaaaggttgc
     3361 gaagcgcgtc actatttatt tttatcttta ccgtaagaat gcagaaacag cggaccggcc
     3421 gttgtttctt tttctttaaa aacaagcaat tattcagtta caactgtttg tactctcgcg
     3481 gggccgggat tataccagcc ccatccgccg tagccatagg gccagccacg accgccataa
     3541 aaccatggat caatcggctg aggcggcata atcacctgct gggttaaatg ccaacgtttg
     3601 taacccgtta cttgcatcac cataaattta tagggcgtat taccgatttt gccgtcaacc
     3661 gcaccggtga ttggcccgac taccgtcacc agttgtccgc ggaaatccac cgggtccaga
     3721 aaaccgttca catcggcata aatgcgaccg cgagaaggtt ctcccagcgt cggtctggct
     3781 ccgctgtcca gtggtacggt ggcaatttcc aggcgggttt tcccttgctg gttttgtacc
     3841 gcaaccactt tgccgccaaa gcgtgcctcc tgaccaacgt acagctgcgg cgcactcatc
     3901 acccgaacta aattttgttg cggcgtggga ctgctgcctt taatggcgtc cggcacagtg
     3961 acacaaccgc tcagcattag cgcaaacgta cctgccagta tgcctttgat aacattcttt
     4021 tgaaccgcca tgatgcgact cctttttttc tcagggcata ctcttaagat tcattctttg
     4081 cccggaagtt tcttccatgc gacgttgtta cgtaaataaa ctggttcagc atgttccacc
     4141 gccaccgttt taccctcggc aaacatctgg cacgcaatcg gcagcatatc ttctgcagcg
     4201 ggcagtaaca cttcgccatc gagcaaaacc agcccgctct ctttaccgag atccggccag
     4261 gcttgccagc ccgtccctac tgtcacccat tcgccggaaa gctgttgcat tcgttcatga
     4321 acgagttcgg gtttgagtac ggcttcggtt tcttcaccgt gccagatacc gttttcatca
     4381 cgctgatatt cggcccagta aacttcgccc attcgcgcgt caatagctgc cagcacgcgg
     4441 gttgcgccgt ttttgcgcca cgccccttgc gccatcgtca ttagcgtgga gacgccaatc
     4501 attggtaact ccgcgccaag tgccagtcct tgcgcaatgc caataccaat gcgcacgccg
     4561 gtgaagctac cggggccgcg cccgtaagcc agagcgttta tgtcagtcag ggaagttccg
     4621 ctggtggtca ggatatcctg caccatcggt aagattcgtt gagtatgttc acgagggcaa
     4681 agctcaaaat gagcgttgac agtaccgtcg ttccacaggg cgacagagca ggcctctgtc
     4741 gcggtatcga tagccagaat tcgcatgggt cttcgtgctt agatcaataa aaaggcgcgc
     4801 atcataccat actccgtaac aaattacctg gaggatggta tcgcaaggaa acgaaccgca
     4861 cgggcaatgt cacgggtgcg cggcgctggc ggcagactgg cgagaaacgt cgcgccgtaa
     4921 gggcgcatca ccagccgatt gtcgcaaatc actaacacgc cgcgatcgtc ggcgtcgcga
     4981 atcaatcgcc ctaccccctg cttgagagta atgaccgcat ccggcagttg tacttcatcg
     5041 aacggatcac caccgcgcaa acggcaatct tccatgcgtg cttttaatag tggatcatcc
     5101 ggcgaggtaa acggcaattt gtcgataatc accaatgaca atgtatcgcc acgcacgtcc
     5161 accccttccc agaaactgct ggttgccaca agaagcgcgt taccggcgct gacaaattgc
     5221 tgcaacagtt gccctttgct ggtttccccc tgcaacaaaa cgggaagcgt catggtagcg
     5281 cggaactgct cggccagatc gcgcatcatg gcgtgcgagg tacaaagcat aaaacaacga
     5341 ccgttgttag cttcgatgat cggtcgcagc attgccgcca gttgccgtgc ggaacccggt
     5401 tgattagttt gcggcaggtt gcgcggcaca tagagtaacg cctggcggct gtaatcaaag
     5461 gggctgggta acagcaacga ctctgccagt tcaatgccaa gccgcgaggt gaaatgatgc
     5521 agatcgtcgt tcaccgacag cgttgctgag gtaaagatcc agctaccggg tttttgcgcc
     5581 attaactctt tgaatttatc cgccacgctg agcggcgtga gagccagagt aaaatggcgc
     5641 gaagtgcatt cgtaccagta gctgtagccc ggctgattga tctcttttag ccgtttcagc
     5701 cgtgtgcgat ataacgtggc gcgttcaaat gccgcatcca gcaaggcgga acgccccagc
     5761 gacagtttcg ccacgtcata acaaagttcc agagtgtcat cgagcagtaa aaatgcccgc
     5821 tgaatttgcg gattagctaa cagctcacgc aggttaccgc gataacctgg ctcaccgagt
     5881 tgcaggcgaa aatcctgcgc actctgggca agacggtcag cgcacttttg taactgctgg
     5941 gtgtctttta attcggtgcg gtaggcgatg gtgatgtctt ttgccaggtc gagcagttgt
     6001 cgactggaga gtgactgacc aaaatactgg ctggcaatgt cgggcagctg gtgggcttcg
     6061 tcgaagatca tgacgtcagc ttccgggatc agttcgccaa atccactctc tttcaccacc
     6121 atatccgcca gaaagagatg atggtttacc accaccacat cggcgtccat cgcttttttg
     6181 cgtgctttga ctacaaagca atctttatac atcgggcagt cgctgccaag gcagttatcg
     6241 ttggtgctgg tgaccagcgg ccacgcctgt gaatcttccg ccacgctgac gcaggtgctg
     6301 atatcaccat cgactgtttg attagaccag gagcgcagca ggatcacatc gcttaatatt
     6361 tgtaccggca gatcgccgcc cgctagcgcc tgttgttcga gacgttcgag gcagaggtag
     6421 tttgagcgcc ctttcagcaa cgccacgttg cccgtatatt tcaacgcctt tgaaaccgtt
     6481 ggcaaatcgc ggctgtagag ctgatcctgc aacgcttttg aaccggtcga gataatgact
     6541 ttctttttcg cccgcagcgc aggggccagg taagcgtagg ttttgcccgt accggttccg
     6601 gcttccacca ccagcggctg gcctttttct atcgcctggg tgacggctac cgccatctgt
     6661 cgctgtggtt ctcgcggctt aaagcctggt atcgctttcg ccaactgacc gtctggtgca
     6721 aaatcgtccg tcacactacc ccctgttgat ttgaacaggg attatgtcag gatgagggcg
     6781 ctttcgccag ttgaagtggt gacggcgacc tcacattgtg gcagtctttg cagcacgaaa
     6841 atggaaggat aatgaggaca aaatgatgac tatcgttcgt atcgatgctg aagcccgctg
     6901 gtctgatgtg gtgatccaca acaacacgct ctactacact ggtgtaccgg aaaacctcga
     6961 cgccgatgct tttgaccaaa ccgccaacac gctggcgcag attgacgccg tgctggaaaa
     7021 acagggcagc aataaatcga gcattctgga tgccaccatt ttcctggctg ataaaaacga
     7081 cttcgcggcg atgaataaag cgtgggatgc ctgggttgtc gcgggtcatg cgccggtgcg
     7141 ctgcacggta caagcgggtt tgatgaaccc gaagtataaa gttgaaatta agattgtggc
     7201 tgcggtgtaa gctttatcga agcaaaataa gtcagacgat aatttatcga taatactggt
     7261 cggttttaca taaatcgacc agagattaag atattactgt tgatgctcct gctaattcat
     7321 tatttatttt tataatttca attttagcta taaaactaac tatataaata aacataacaa
     7381 tgccgttgtg ttcgcccttc gattagcgtc tctggttttc tttcttagct gaacaatttg
     7441 gttatagcgt gagaactgac cgttaatttt ctccctctta ctggataaca caccatgagc
     7501 tgctaccttt aaaacacctg aatgtcaaca ggttaactcg cgccatatcg ttataatccc
     7561 tttgatatta gatgcaaatt aaggtcatat atagccttat cgataacatg gttaatttta
     7621 aggataagaa tatgcctgct gtaatagata aagccctgga tttcattggt gccatggatg
     7681 tatcagcacc aacaccaagt tcgatgaatg aaagtacggc gaagggaatc tttaaatatt
     7741 taaaagaact gggagtaccc gcgagtgccg ctgatattac gacgcgagct gacctggaag
     7801 gctggaaccc ggggttcacg gaaaaaatgg ttggatgggc aaaaaaaatg gagtctggtg
     7861 aacgcattgt gattaaaaat cctgaatact tttcaacata tatgcaggaa gaactaaaag
     7921 cactggtcta agttaaattt acatcagcat aaatgggtca ggacgctttt aatcacatat
     7981 gaaaagcgtc ctttttctca caatcaaata accatcggct tctataacga aagcgatacg
     8041 gataagtgtg ttactcgtct tcatcctcaa aacgcgccat gattcgctcg ccggaatggt
     8101 tggcacgcag ctcttctgcc accagcgcga ttgcctggcc gctgctcatc ccttgagcca
     8161 tcagttcctg gatccgctcg acggcttttt gctgctgttc atgggtgagt gaaggtaaac
     8221 ctgcaaacat tgttaactcc tgctaaattg ttggcgctaa ttatttcatg ctacccggca
     8281 catagccagt agagtcagga ctgatgaaga cgttatctcc cgctgtgatt actttaccct
     8341 ggcgtcagga cgccgctgaa ttttatttct cacgcttaag ccacctgccg tgggcgatgc
     8401 ttttacactc cggctatgcc gatcatccat atagccgctt tgatattgtg gtcgccgagc
     8461 cgatttgcac tttaaccact ttcggtaaag aaaccgttgt tagtgaaagc gaaaaacgca
     8521 caacgaccac tgatgacccg ctacaggtgc tccagcaggt gctggatcgc gcagacattc
     8581 gcccagcgca taacgaagat ttgccatttc agggcggcgc gctggggttg tttggctacg
     8641 atctgggccg ccgttttgag tcactgccag aaattgcgga acaagatatc gttctgccgg
     8701 atatggcagt gggtatctac gactgggcgc tggttgtcga ccaccagcgt aaaacagttt
     8761 ctttgctgag tcataatgat gtcaatgccc gtcggacctg gctggaaaac cagcaattct
     8821 ccccgcagga agatttcgcg cttacttccg actggcaatc caatatgacc cgcgagctgt
     8881 acggcgaaaa atttcgcaaa gtacaggaat atctgcacag cggtgattgc tatcaggtga
     8941 atctcgccca gcgttttcat gcgacctatt ctggcgatga atggcaggca ttccttcagc
     9001 ttaatcaggc caatcgcgcg ccgtttagcg cttttttacg tcttgaacag gctgctattt
     9061 taagcctttc accagagcgg tttattcttt gtgataatgg tgaaatccag acccgcccga
     9121 ttaaaggcac gctaccacgc ctgcccgatc ttcaggaaga tagcaaacaa gcagaaaaac
     9181 tggcgaactc aacgaaagat cgtgccgaaa atctgatgat tgtcgattta atgcgtaatg
     9241 atatcggtcg tgttgccgta gcaggttcgg taaaagtacc agagctgttc gtggtggaac
     9301 ccttccctgc cgtgcatcat ctggtcagca ccataacggc gcaactacca gaacagttac
     9361 acgccagcga tctgttgcgc gcggcttttc ctggtggctc aataaccggg gcaccgaaag
     9421 tacgggctat ggaaattatc gacgaactgg aaccgcaccg acgcaatgcc tggtgcggca
     9481 gcattggcta tttgagcttt tgcggcaaca tggacaccag cattactatc cgcacgctga
     9541 ctgccattaa cggacaaatt tactgctctg cgggcggtgg aattgtcgcc gatagccagg
     9601 aagaagcgga atatcaggaa acttttgata aagttaataa gatattacgc caactggaga
     9661 agtaagacgt ggaataccgt agcctgacgc ttgatgattt tttatcgcgc tttcaacttt
     9721 tgcgcccgca aattaaccgg gaaaccctaa atcatcgtca ggctgccgtg ttaatcccca
     9781 tcgtccgtcg accgcaaccg gggttgttgc tgactcagcg ttcgattcat ctgcgtaaac
     9841 acgctggaca agtggcattc cctggaggcg cagtcgatga cacggacgca tccgttatcg
     9901 ccgccgcgct gcgcgaagct gaagaagagg tcgctatacc gccttccgcc gttgaagtta
     9961 tcggcgtgct gccgcccgtc gatagcgtaa ctggctacca ggtaaccccg gtggtcggca
    10021 ttatcccgcc cgatctgccg tatcgcgcca gtgaagatga agtctcggca gtgtttgaaa
    10081 tgccgctcgc ccaggcatta catctgggtc gttatcaccc tttagatatc taccgccgtg
    10141 gcgattcaca tcgggtatgg ctatcctggt acgaacagta ttttgtatgg ggaatgaccg
    10201 caggcataat tcgtgaactg gcgctgcaaa ttggtgtgaa accctgacta tacttatctt
    10261 tacatctaca aaacactact tgagacaatc atcgcaatat tagttaaatc gcggtttttg
    10321 attagtttaa ttcatgtgaa tagttaagcc agtcgccgcg ttccctctta cactatgcgc
    10381 tgttattagt tcgttactgg aagtccagtc accttgtcag gagtattatc gtgattagtc
    10441 tattcgacat gtttaaggtg gggattggtc cctcatcttc ccataccgta gggcctatga
    10501 aggcgggtaa acagttcgtc gatgatctgg tcgaaaaagg cttactggat agcgttactc
    10561 gcgttgccgt ggacgtttat ggttcactgt cgctgacggg taaaggccac cacaccgata
    10621 tcgccattat tatgggtctt gcaggtaacg aacctgccac cgtggatatc gacagtattc
    10681 ccggttttat tcgcgacgta gaagagcgcg aacgtctgct gctggcacag ggaccgcatg
    10741 aagtggattt cccgcgcgac aacgggatgc gttttcataa cggtaacctg ccgctgcatg
    10801 aaaacggtat gcaaatccac gcctataacg gcgatgaagt cgtctacagc aaaacttatt
    10861 attccatcgg cggcggtttt atcgtcgatg aagaacactt tggtcaggat gctgccaacg
    10921 aagtgagcgt gccgtatccg ttcaaatctg ccaccgaact tctcgcgtac tgtaatgaaa
    10981 ccggctattc gctgtctggt ctcgctatgc aaaacgaact ggcactgcac agcaagaaag
    11041 agatcgacga gtatttcgcg catgtctggc aaaccatgca ggcatgtatc gatcgcggga
    11101 tgaataccga aggcgtactg ccaggcccgc tgcgcgtgcc acgtcgtgcg tcggccctgc
    11161 gccggatgct ggtttccagc gataaactgt ctaacgatcc gatgaatgtc attgactggg
    11221 taaacatgtt tgcgctggca gttaacgaag aaaacgccgc cggtggccgt gtggtaactg
    11281 cgccaactaa cggtgcctgc ggtatcgttc cggcagtgct ggcatactat gaccacttta
    11341 ttgaatcagt tagcccagac atctataccc gttactttat ggcagcgggc gcgattggtg
    11401 cactgtataa aatgaacgcg tctatttccg gtgcggaagt cggttgccag ggcgaagtag
    11461 gtgttgcctg ttcaatggct gctgcaggcc ttgccgagct gttgggcggt agcccggaac
    11521 aggtttgtgt ggcagcggaa attggcatgg agcacaacct tggtttgacc tgcgaccccg
    11581 ttgcagggca agttcaggtg ccgtgcattg aacgtaatgc cattgcctct gtgaaggcga
    11641 ttaacgccgc acggatggct ctgcgccgca ccagtgcacc gcgcgtctcg ctggataagg
    11701 tcatcgaaac gatgtacgaa accggtaagg acatgaacgc taaataccgc gaaacctcac
    11761 gcggtggtct ggcaatcaaa gtccagtgtg actaatactt tatcctcgcc catctgcaac
    11821 ggatgggcga atttatcccc gctttctcgt ctgctgtaat tttccccact acacttccac
    11881 tgttgcgtca ggcgtttgtc gccatacgct tacagggtgg cccgcatgca aaaagcacaa
    11941 cggatcatta aaacctatcg ccgtaatcga atgattgttt gtacgatttg cgcactcgtt
    12001 acgctcgctt cgaccctgag cgtgcgattt atttcacagc gtaacttaaa tcaacaacgg
    12061 gtagtacaat tcgccaatca cgctgtagag gaattagata aagtactgct tcccctacag
    12121 gcaggtagcg aagtcttact tccgctaatt ggtctgccct gctctgtcgc ccatttgcca
    12181 ttacgtaaac aggctgcaaa actccaaact gtgcgatcca ttggcctggt gcaagacggc
    12241 acactttatt gctccagcat ttttggttat cgcaatgtgc ccgtcgtgga cattctggct
    12301 gaacttcctg caccgcaacc acttttacga ctgacgaccg accgtgccct gataaaaggc
    12361 agtccggttt tgattcaatg gacgcctgca gcgggcagta gcaatgctgg ggtcatggag
    12421 atgattaaca tcgacttact gacggcaatg ctccttgaac cacaactgcc gcaaatcagt
    12481 agcgccagcc tgacggtgga cagtcggcat ttgctctatg gtattgggct ggtagattcc
    12541 cttccgcaac ctgaacacaa tgaaaactat caggtttctt cgcaacgctt tccttttacc
    12601 attaacgtta atggcccggg ggctacggcg ctggcatggc actatcttcc aacacaatta
    12661 ccgctggcgg tgctgctaag tttactggtg ggctacatcg cctggctggc gaccgcttac
    12721 cgaatgagct tttcccgcga gatcaatctg ggcctggcgc aacatgagtt cgaattgttc
    12781 tgtcagcctt tgcttaatgc gcgcagccag caatgtattg gtgtagagat tttgctgcgc
    12841 tggaacaatc cgcgtcaggg ctggatttca ccggatgtgt ttattcctat cgcggaagaa
    12901 catcatttaa ttgtgccact aacccgctat gtgatggcag aaaccattcg tcagcgccat
    12961 gttttcccaa tgagtagtca gtttcatgtt ggcattaacg tcgcacccag ccattttcgc
    13021 cgtggtgtgc tgataaaaga tctcaatcag tactggttta gcgctcaccc gattcagcaa
    13081 ctgatcctcg aaatcaccga acgcgatgcc ttactggatg ttgattaccg gattgcccgc
    13141 gagctgcatc gtaaaaacgt caaactggcg attgatgact tcggcaccgg caacagttca
    13201 ttttcctggc ttgaaacatt acgtcctgac gttctgaaaa ttgataagtc atttaccgca
    13261 gctataggtt ctgatgcggt taactcgacg gtgaccgata tcatcatcgc actggggcaa
    13321 agactgaata ttgaactggt ggcggagggc gtagaaacgc aagaacaggc gaagtatttg
    13381 cgcagtcatg gcgtgcatat tttgcaaggg tatttgtacg cacagccgat gccgctacgt
    13441 gattttccca aatggctggc gggcagccaa ccgccgcccg cccggcataa tggacatatc
    13501 acacccatta tgccgttacg ttaacgctta ctcatcttca tcgtgcgccg gttgctcttt
    13561 aacaatgcga accagatcaa cacgataatc attggcttca atgatggtga tatgcagtgg
    13621 ccctacatca atcacatcgc ccacacgggg aatgtgacca tttgccgaga tcacgaggcc
    13681 cgcgaccgtc gcgatatcat cgtcatcggc aaggtgctca acatcaagcg cctgctgcaa
    13741 ggcatgcaaa tctgtaccgc cttttaccag ccagccgtca ccgtcggtaa tgatttccgg
    13801 cgtttcgtca gcgtccggga attcacccgc aatggcttcc agcacatcca gcggcgtgac
    13861 cagaccttgt accacaccaa actcgttggt cacaataaca aagctcccgc gagcacgacg
    13921 cagcacgccc agcaggttga tcggatcgag ggtttccggg acgataatcg ccggagacgc
    13981 cgaagcaatc gccgccacat caacgccctc ttccagcgcc accagcagtt ctttagcacg
    14041 tacgatgccg ataatttcat ccagttcacc gcgacatacc gggaacaaac tgtgcggtga
    14101 ggagagcagc tgctcgcgga tttcatcgac cccgagatta gcatcaaccc agcttatttc
    14161 accgcgcggc gtcatgatcc cgcgcagaga acgcgacgcc agcgtcagta cgccgttaat
    14221 catgtaacgt tcttcttcgg caaacgcacc ttccgggatc ggcatcggca tcgggttatc
    14281 ggcatcgtgc tgaacattgg cctgacgttt cccgcccatc aaacgcagga tggcatcggc
    14341 agtacgcgct cgcagcggca aagtcgactg gtgacgaata aagttgcgac gcgcaatctg
    14401 gttaaacact tcgatgatga tcgagaagcc aatcgcggca tacaggtaac ctttcggaat
    14461 gtggaaaccg aaaccttctg ccaccagact cagaccaatc attaacagga agctcagaca
    14521 gagcaccacc accgtggggt gctggttaac gaatcgcgtc agcggttttg atgccagcag
    14581 cataaccgcc atcgcaatca ctaccgccgc catcatcacc ggcaaatggt taaccatccc
    14641 tactgcagta attaccgcat ccaacgagaa gacggcgtca aggatgacaa tctgtgtgac
    14701 gaccacccag aaactggcgt agcctttacc gtggccggaa tcatgatcgc ggttttccag
    14761 ccgttcatgc agttcggttg ttgctttgaa cagcaagaat atgcccccga acaacataat
    14821 caggtcgcgt ccggagaagg agaaatccat gacggtaaat agcggtttgg tcagcgtgac
    14881 catccatgaa atcagcgaca gcagccccag acgcataatc agcgccagtg ataatcccag
    14941 caaacgcgct ttatcgcgtt gttttggcgg cagtttgtca gcaagaatgg cgatgaagac
    15001 caggttatcg atacccagca caatttcgag aacaacaagc gtgagtagcc ccgcccaaat
    15061 cgaggggtcc attaagaatt ccatgacaag ctcctgctta aggaatagct attcgacgcc
    15121 agaaataatg caggcgtaac gacaaaatgc aaacgaaagg tgcggcataa agtgccagaa
    15181 aggcaggcgt taaaaggcct gatgctgaaa tgacgtcggt gacgatccat actgcgggct
    15241 actgccctat actccatggt tgttaaacag gagttaaaca tatcagagac gcctctgatt
    15301 tggcaaagat ttaccttcct ttgcaaacga atgtgacaag gatattttac ctttcgaaat
    15361 ttctgctaat cgaaagttaa attacggatc ttcatcacat aaaataattt tttcgatatc
    15421 taaaataaat cgcgaaacgc aggggttttt ggttgtagcc cttatctgaa tcgattcgat
    15481 tgtggacgac gattcaaaaa tacatctggc acgttgaggt gttaacgata ataaaggagg
    15541 tagcaagtga ccattgctat tgttataggc acacatggtt gggctgcaga gcagttgctt
    15601 aaaacggcag aaatgctgtt aggcgagcag gaaaacgtcg gctggatcga tttcgttcca
    15661 ggtgaaaatg ccgaaacgct gattgaaaag tacaacgctc agttggcaaa actcgacacc
    15721 actaaaggcg tgctgtttct cgttgataca tggggaggca gcccattcaa tgctgccagc
    15781 cgcattgtcg tcgacaaaga gcattatgaa gtcattacag gcgttaacat tccaatgctc
    15841 gtggaaacgt taatggcccg tgatgatgac ccaagctttg atgaactggt tgcgctggca
    15901 gtagaaacag gccgtgaagg cgtgaaagct ctgaaagcca aaccggttga aaaagccgcg
    15961 ccagcacccg ctgccgcagc accaaaagcg gctccaactc cggcaaaacc aatgggacca
    16021 aacgactaca tggttattgg ccttgcgcgt atcgacgacc gtctgattca cggtcaggtc
    16081 gccacccgct ggaccaaaga aaccaatgtc tcccgtatta ttgttgttag tgatgaagtg
    16141 gctgcggata ccgttcgtaa gacactgctc acccaggttg cacctccggg cgtaacagca
    16201 cacgtagttg atgttgccaa aatgattcgc gtctacaaca acccgaaata tgctggcgaa
    16261 cgcgtaatgc tgttatttac caacccaaca gatgtagagc gtctcgttga aggcggcgtg
    16321 aaaatcacct ctgttaacgt cggtggtatg gcattccgtc agggtaaaac ccaggtgaat
    16381 aacgcggttt cggttgatga aaaagatatc gaggcgttca agaaactgaa tgcgcgcggt
    16441 attgagctgg aagtccgtaa ggtttccacc gatccgaaac tgaaaatgat ggatctgatc
    16501 agcaaaatcg ataagtaacg tattgtgttg attatcactc agttttcaca cttaagtctt
    16561 acgtaaacag gagaagtaca atggagatta ccactcttca aattgtgctg gtatttatcg
    16621 tagcctgtat cgcaggtatg ggatcaatcc tcgatgaatt tcagtttcac cgtccgctaa
    16681 tcgcgtgtac cctggtgggt atcgttcttg gagatatgaa aaccggtatt attatcggtg
    16741 gtacgctgga aatgatcgcg ctgggctgga tgaacatcgg tgctgccgtt gcgcctgacg
    16801 ccgccctggc ttctatcatt tctaccattc tggttatcgc aggtcatcag agcattggtg
    16861 caggtatcgc actggcaatc cctctggccg ctgcaggcca ggtactgacc atcatcgttc
    16921 gtactattac cgttgctttc cagcacgctg cggataaggc tgctgataac ggcaacctga
    16981 cagcaatttc atggatccac gtttcttctc tgttcctgca agcaatgcgt gtggctattc
    17041 cggccgtcat cgttgcgctg tctgttggta ccagcgaagt acagaacatg ctgaatgcga
    17101 ttccggaagt ggtgaccaat ggtctgaata tcgccggtgg catgatcgtg gtggttggtt
    17161 atgcgatggt tatcaacatg atgcgtgctg gctacctgat gccgttcttc tacctcggct
    17221 tcgtaaccgc agcattcacc aactttaacc tggttgctct gggtgtgatt ggtactgtta
    17281 tggcagtgct ctacatccaa cttagcccga aatacaaccg cgtagccggt gcgcctgctc
    17341 aggctgctgg taacaacgat ctcgataacg aactggacta acaggtgagc gaaatggttg
    17401 atacaactca aactaccacc gagaaaaaac tcactcaaag tgatattcgt ggcgtcttcc
    17461 tgcgttctaa cctctttcag ggttcatgga acttcgaacg tatgcaggca ctgggtttct
    17521 gcttctctat ggtaccggca attcgtcgcc tctaccctga gaacaacgaa gctcgtaaac
    17581 aagctattcg ccgtcacctg gagttcttta acacccagcc attcgtggct gcgccgattc
    17641 tcggcgtaac cctggcactg gaagaacagc gtgctaatgg cgcagagatc gatgacggtg
    17701 ctatcaacgg tatcaaagtc ggtttgatgg ggccgctggc tggtgtaggc gacccgatct
    17761 tctggggaac ggtacgtccg gtatttgcag cgctgggggc tggtatcgcg atgagcggca
    17821 gcctgttagg tccgctgctg ttcttcatcc tgtttaacct ggtgcgtctg gcaacccgtt
    17881 actacggcgt agcgtatggt tactccaaag gtatcgatat cgttaaagat atgggtggtg
    17941 gcttcctgca aaaactgacg gaaggggcgt ctatcctcgg cctgtttgtc atgggggcat
    18001 tggttaataa gtggacacat gtcaacatcc cgctggtggt ctctcgcatt actgaccaga
    18061 cgggcaaaga acacgttacc actgtccaga ctattctgga ccagttaatg ccaggccttg
    18121 tgccactgct gctgaccttt gcttgtatgt ggctgttgcg caaaaaagtt aacccgctgt
    18181 ggatcatcgt tggcttcttc gtcatcggta tcgctggtta cgcttgcggc ctgctgggac
    18241 tgtaagactg ttgtacacta ccggggcctt ttggccccgt ttttttatct ggaggattaa
    18301 tgacaatcac ggacctggta ctgattcttt tcatcgccgc acttctggcc ttcgcgatct
    18361 acgatcagtt catcatgcac cgccgtaacg gccccaccct gctggcaatt cctttgctcc
    18421 ggcgtggtcg catcgatagc gttatcttcg tcggattgat tgtcattctt atctataaca
    18481 acgtcacgaa tcatggcgcg cttataacaa catggttatt aagtgcactg gctctgatgg
    18541 gtttttatat attctggatt cgcgttccga agatcatctt taaacaaaaa ggttttttct
    18601 tcgccaatgt ctggattgaa tatagccgaa tcaaagcgat gaacttgtcg gaagatggcg
    18661 tgttggtgat gcaattagaa cagcgtcggc tgttaatccg cgttcgaaat atcgacgatt
    18721 tggaaaaaat ttataagctt ctcgtttcaa ctcaataagt tatgaattta gccaaagcta
    18781 tgtttagtgt atttttaata atcagacata gcttaggcta tattacctct tcccttattt
    18841 gttttttatt ttaacgtttc attgatatat aaatataaat gaaaatcgtt atcaataaaa
    18901 caataaaata atatattcca acagttgtct tatattctca aaatatgtta aggttgcgcc
    18961 ctcatttggg gagtagctga tttccagatt ccggaaatgt acgtgtcaac atactcgttg
    19021 caaaacgtgg cacgtacgga ctgaacactt tcagtcaggc gagaccatat gcacatcaat
    19081 cgctatgcct gcatgaacgc gaaaaccatc gcggcttgtt gttcatgtat gcatgtttgc
    19141 tgggggcgat gatgtgtttt atggataccc cggtcaggac gttgtcatga atatcactgc
    19201 tactgttctt cttgcgtttg gtatgtcgat ggatgccttt gctgcatcaa tcggtaaagg
    19261 tgccaccctc cataaaccga aattttctga agcattgcga accggcctta tttttggtgc
    19321 cgtcgaaacc ctgacgccgc tgatcggctg gggaatgggc atgttagcca gtcggtttgt
    19381 ccttgaatgg aaccactgga ttgcgtttgt gctgctgata tttctcggcg ggcgaatgat
    19441 tattgagggt tttcgtggcg cagatgatga agatgaagaa ccgcgccgtc gacacggttt
    19501 ctggttactg gtaaccactg cgattgccac cagcctggat gccatggctg tgggtgttgg
    19561 tcttgctttc ctgcaggtta acattatcgc gaccgcattg gccattggtt gtgcaacctt
    19621 gattatgtcg acattaggga tgatggttgg tcgctttatc ggctcaatta ttgggaaaaa
    19681 agcggaaatt ctcggcgggc tggtactgat cggtattggc gtccagatcc tctggacgca
    19741 cttccacggt taataagaac gctgccagag gtgaatatta aagtccgtct ggcagtcgaa
    19801 cacttctttt gctgccagtg cttgccagac ttcaggcttc gcacgccagg caaacggcgt
    19861 catctgcaat aatgcgacgg cttcatcacc gcgaagacgc atcagataac acaactcatc
    19921 actctgctgt aatgtaaaac ctttcagttg ttctgcatga ggtgcatgaa gatgtacttc
    19981 attgtaaatc agccccttca gctccattaa gtgtctcggt cccggcgtgg cagtaatgac
    20041 ccaaccgccg ggcttcacta ctcgcgctag ttcttctgct ttacacggcg cgtaaatacg
    20101 tattatggcg tccatactgg tatcggaaaa cggcaaacgg tggctggaag caacacaaaa
    20161 agtgacctgc ggatagcgtt tcgccgccgc ttttatcgct accttcgaga catccagacc
    20221 aaacgtggtg atttcgggca acgcatcagc aaatgcgtgt gtgtaatacc cttcaccaca
    20281 gccaatatcc agcaccgccg cggcctttgc atcaagccgt tcccgcagtt ggccgacaat
    20341 tgcatcacgc agcggctgat aatgtccggc atctaagaat gcgcggcgtg cttgcatcat
    20401 ttccgcgctg tcgcccggat cacgagaccg tttatgctga acgggcagca gattgacata
    20461 cccttctttc gccatatcaa actgatgtcg caggggacag atatagctgt ttttttcacg
    20521 cgaaagaggc tgatggcaaa gtggacagga aaacgacatg acaactccgg caggatacta
    20581 aaggccgcaa gtgtaacgcg aatcatgcgg cgggcaaata tccgaacgac tggagcgaag
    20641 ataaaacaaa gtaaaaagcc tcgcattcga cgaggcttta tagcgagatt gaagcgtatt
    20701 cacacttcag atcagtggat tcgatcagat agctgttacg ttaacagctg ccggaccttt
    20761 ctgaccgtcc tgaatttcga actcaacgtt ctgaccttca gccagagttt tgaagccatt
    20821 accctggata gcggagaagt gtacgaacac atctttgctg ccatcagccg gagtaatgaa
    20881 gccaaaacct ttagactcgt tgaaccactt aacctgacct ttaatctttg ccatttgaaa
    20941 aattccttag attgttttct tcgcccgcag gcataacata gataaaactg acacattact
    21001 gcatgaggca ccaatataag gctcggcaga gaagcggtat tcaacgtcaa cgtgtttact
    21061 caggacttct ttactgaaaa tgccacacat aaacaaaact gtacctcgtt taacccgaaa
    21121 tctgttatca catacaacgt taattatggc aagccatttt taaacatgtc tcgatcagac
    21181 gcacaaatct cgatacgtct tccacttttt tgcacactta tgcaacggaa tacgcgccga
    21241 tatatcattg tgcttaacct tgccagttca ggcagatact taaactgacg tattttctaa
    21301 catagttcaa taagttcagt tgttccaggc cagtgggaga agttattaca tagtgcgtgc
    21361 aatatcacat tttctactat gcaatgaata aaaagttata tcactttttc tcataaaaca
    21421 gtcagttaac ggctatcaat taccctaa
//
LOCUS       DAZFFE010000083        21059 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFFE010000083 DAZFFE010000000
VERSION     DAZFFE010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.083"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(160..822)
                     /gene="fsa"
                     /locus_tag="ACHJ69_003339"
     CDS             complement(160..822)
                     /gene="fsa"
                     /locus_tag="ACHJ69_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO5016261.1"
                     /translation="MELYLDTSDVVAVTALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            952..1851
                     /gene="ybiY"
                     /locus_tag="ACHJ69_003340"
     CDS             952..1851
                     /gene="ybiY"
                     /locus_tag="ACHJ69_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO5016262.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCKLCAKTAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            1857..4289
                     /gene="ybiW"
                     /locus_tag="ACHJ69_003341"
     CDS             1857..4289
                     /gene="ybiW"
                     /locus_tag="ACHJ69_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO5016263.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSHINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPIGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            4435..5250
                     /gene="ybiV"
                     /locus_tag="ACHJ69_003342"
     CDS             4435..5250
                     /gene="ybiV"
                     /locus_tag="ACHJ69_003342"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO5016264.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMSNAAENIKQIARYATDDNNHEGALNVIQTVLENKPPFNL"
     gene            complement(5450..7042)
                     /gene="ybiT"
                     /locus_tag="ACHJ69_003343"
     CDS             complement(5450..7042)
                     /gene="ybiT"
                     /locus_tag="ACHJ69_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO5016265.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            7261..8181
                     /gene="ldtB"
                     /locus_tag="ACHJ69_003344"
     CDS             7261..8181
                     /gene="ldtB"
                     /locus_tag="ACHJ69_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO5016266.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(8239..9357)
                     /gene="ybiR"
                     /locus_tag="ACHJ69_003345"
     CDS             complement(8239..9357)
                     /gene="ybiR"
                     /locus_tag="ACHJ69_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO5016267.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPVLQGVLSNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAG"
     gene            complement(9354..9821)
                     /gene="mntR"
                     /locus_tag="ACHJ69_003346"
     CDS             complement(9354..9821)
                     /gene="mntR"
                     /locus_tag="ACHJ69_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO5016268.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            10007..10135
                     /gene="mntS"
                     /locus_tag="ACHJ69_003347"
     CDS             10007..10135
                     /gene="mntS"
                     /locus_tag="ACHJ69_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO5016269.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            10407..11990
                     /gene="opgE"
                     /locus_tag="ACHJ69_003348"
     CDS             10407..11990
                     /gene="opgE"
                     /locus_tag="ACHJ69_003348"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO5016270.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGIFIFALGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(12040..12555)
                     /gene="ompX"
                     /locus_tag="ACHJ69_003349"
     CDS             complement(12040..12555)
                     /gene="ompX"
                     /locus_tag="ACHJ69_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO5016271.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(12608..12673)
                     /locus_tag="ACHJ69_003350"
     CDS             complement(12608..12673)
                     /locus_tag="ACHJ69_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016272.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            12908..13795
                     /gene="rhtA"
                     /locus_tag="ACHJ69_003351"
     CDS             12908..13795
                     /gene="rhtA"
                     /locus_tag="ACHJ69_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO5016273.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGTLQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            14095..14598
                     /gene="dps"
                     /locus_tag="ACHJ69_003352"
                     /gene_synonym="pexB"
     CDS             14095..14598
                     /gene="dps"
                     /locus_tag="ACHJ69_003352"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO5016274.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            15002..15748
                     /gene="glnH"
                     /locus_tag="ACHJ69_003353"
     CDS             15002..15748
                     /gene="glnH"
                     /locus_tag="ACHJ69_003353"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO5016275.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            15887..16546
                     /gene="glnP"
                     /locus_tag="ACHJ69_003354"
     CDS             15887..16546
                     /gene="glnP"
                     /locus_tag="ACHJ69_003354"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO5016276.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            16543..17265
                     /gene="glnQ"
                     /locus_tag="ACHJ69_003355"
     CDS             16543..17265
                     /gene="glnQ"
                     /locus_tag="ACHJ69_003355"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO5016277.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            17382..19607
                     /gene="ybiO"
                     /locus_tag="ACHJ69_003356"
     CDS             17382..19607
                     /gene="ybiO"
                     /locus_tag="ACHJ69_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO5016278.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGITTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(19604..20530)
                     /gene="rlmF"
                     /locus_tag="ACHJ69_003357"
     CDS             complement(19604..20530)
                     /gene="rlmF"
                     /locus_tag="ACHJ69_003357"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO5016279.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            20806..20985
                     /locus_tag="ACHJ69_003358"
                     /pseudo
     CDS             20806..20985
                     /locus_tag="ACHJ69_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            complement(21002..21058)
                     /locus_tag="ACHJ69_003359"
     CDS             complement(21002..21058)
                     /locus_tag="ACHJ69_003359"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO5016280.1"
                     /translation="MELHDKVIGHYLNIKHYQ"
BASE COUNT         5162 a         5523 c         5183 g         5191 t
ORIGIN      
        1 ttaagcgtcg catccggcat ttgctccaga cgccagatgc ggcgtgaacg ccttatctgg
       61 cctacggttc tgacgcagat ttccaggcct gatagacgcg gcaggcatcg gtgcactccg
      121 ccacgtcatc agcaatttat gaggatgcag aacgtccggt taaatcgacg ttctgccaaa
      181 cgctcccagc cagtcctgtt caaacttcgc cacagcggca tcaaccgccg gatagctaat
      241 catctgttgt gccacatcca gcggcagagt aattgattca catcctgcca gtaagcagtc
      301 cagcgcctga cgcggggttt tgaaactcgc ggctaacact ttcgcctgcg gcgcatgcat
      361 tttcaataac tggtgtaagt cggtcacagt ctgaataccg ctaccgccct gagcatcaat
      421 acgattaacg taaggcgcaa catattccgc acctgccagc gccgacagta gcccttgtgc
      481 tgcgccatat accgcggttc ccagcgtcgg aatcccttcc gcttttaaca tcttaatagc
      541 tgccagcccc tcagcggtca ccggaacttt caccacgata tccgcaataa tagaacgcaa
      601 tttacgtgcg tcattaacca tcccttcagc agtggtagcc attacctggg caaacagacg
      661 cccctgaccg cccatcgcct cgtgaagttg cggaagcaca acctccagcg gttttttacc
      721 cgctgcgata atgcttgggt tagtggtcac acccgccagc ggaaaaatac gtgacagcgc
      781 cgtcaccgca acaacgtctg aagtatccag atacagttcc ataaccatcc tcaaaatgta
      841 ctttttgtct gaatgtagac tatcacggga aaactctctg aacagttgac gcacatcaat
      901 ctaacttcat tcgaaagtat tttaatgttt gaacgaaaca gagaggcaac tatgattttc
      961 aatattcaac gctactcgac ccatgatggc cccggtatcc gcacggtcgt atttcttaaa
     1021 ggctgttcgc tgggctgccg ctggtgtcag aacccggaaa gccgcgcccg cacgcaggat
     1081 ctgctgtatg acccacgact ctgtctggaa ggctgtaagc tgtgcgctaa gaccgcaccg
     1141 gaagtgattg agcgcgcgtt gaatggtttg cttattcatc gggaaaagtt aaccccggag
     1201 cattttacgg cgctaaccga ctgctgcccg acacaggcgt taaccgtgtg tggtgaagtg
     1261 aaaagcgttg aggagatcat ggcaaccgtt ttgcgcgata aaccgtttta cgatcgcagc
     1321 ggcggcggct taacgctttc tggcggtgaa ccgtttatgc agccggaaat ggcgacggcg
     1381 ctgctgcaag ccagccacga ggccggcatt catactgcgg tagaaacctg tctgcatgtg
     1441 ccgtggaaat atatcgcccc ttctctgccc tatatcgatc tgtttcttgc cgatttaaaa
     1501 cacgttgccg acgcgccgtt taaacagtgg accgacggta acgccgccag ggtgctggat
     1561 aacctgaaaa aactcgccgc agccggcaaa aaaatcatta tccgcgtgcc actgattcag
     1621 ggctttaatg ccgacgaaac ttctgtgaaa gccattaccg attttgccgc cgacgagctg
     1681 cacgttagcg aaattcattt tctgccctac cacacactgg gcatcaacaa atatcactta
     1741 cttaatctgc cctatgacgc cccggaaaaa ccgcttgatg cgccagaact gctcgacttt
     1801 gcccagcagt atgcctgcca gaaagggtta accgcgacct tacgaggata acaatcatga
     1861 caacactaaa actggatacg ctcagcgacc gcattaaagc gcacaaaaat gcgctggtgc
     1921 atattgtgaa accgccggtc tgtaccgaac gagcacaaca ctataccgag atgtatcaac
     1981 aacatctcga taagccaatc ccggtacgtc gcgcgctggc actggcgcat cacctggcga
     2041 atcgcaccat ctggatcaag cacgatgagt tgatcattgg caaccaggca agcgaagttc
     2101 gcgccgcgcc gatcttcccg gaatataccg tctcgtggat cgaaaaagag attgatgatc
     2161 tggcagatcg tcccggtgct ggttttgcgg tgagcgaaga gaacaaacgc gttctgcatg
     2221 aagtgtgccc gtggtggcgc ggtcagaccg tacaggatcg ctgctacggc atgtttaccg
     2281 atgagcaaaa aggtctgctg gcgaccggaa tcattaaagc ggagggcaat atgacctccg
     2341 gcgatgcgca cctggcggtc aatttcccgc tactgctgga aaaaggtctt gatggtctgc
     2401 gtgagaaagt ggcggaacgt cgctcgcaca tcaatctgac ggttctggaa gatctgcacg
     2461 gcgagcaatt cctgaaagcg attgatatcg tgctggtggc agtcagtgaa cacattgaac
     2521 gtttcgctgc cctggcgcgt gaaatggccg cgaccgaaac ccgcgaaagc cgtcgcgatg
     2581 aactgctgac gatagcagaa aactgcgatc ttatcgccca ccagccgccg cagactttct
     2641 ggcaggcgct gcaactatgt tacttcatcc agttgatttt gcagatcgaa tctaacggtc
     2701 actcggtatc gtttggtcgt atggaccagt atctctatcc atactatcgc cgtgacgttg
     2761 aactgaacca gacactggat cgcgaacacg ccatcgagat gctgcatagc tgctggttga
     2821 aattgctgga agtgaacaaa atccgctccg gctcacactc aaaagcctct gcgggaagtc
     2881 cgctgtatca gaacgtcacc attggcgggc aaaatctggt tgatggtcaa ccgatggacg
     2941 cggtaaatcc actctcttat gcgatcctcg aatcttgcgg tcgcctgcgt tcgactcagc
     3001 ctaacctcag cgtgcgttac catgcgggta tgagtaacga tttcctcgac gcctgtgttc
     3061 aggtgatccg ctgcggcttc gggatgccgg cgttcaacaa cgacgaaatc gtcatcccgg
     3121 aatttattaa actcggtatt gaaccgcagg acgcttacga ctatgcagcg attggttgta
     3181 tcgaaaccgc cgtcggtggc aaatggggct atcgctgtac cggcatgagc tttatcaact
     3241 tcgcccgcgt gatgctggcg gcgctggaag gcggtcgtga tgccaccagc ggcaaagtgt
     3301 tcctgccaca agaaaaagcg ttgtcagcgg gtaacttcaa caacttcgat gaagtaatgg
     3361 acgcatggga tacgcaaatc cgttactaca cgcgtaaatc aatcgaaatc gaatatgtcg
     3421 tcgataccat gctggaagag aacgtacacg atattctctg ctcggcgctg gtcgatgact
     3481 gtattgagcg agcgaaaagt atcaagcaag gcggcgcgaa atatgactgg gtttctggcc
     3541 tgcaggtcgg cattgctaac ctcggcaaca gcctggcggc agtgaagaaa ctggtatttg
     3601 aacagggcgc gattggtcag caacagctgg ctgccgcact ggcggatgac ttcgacggcc
     3661 tgactcacga gcagctgcgt cagcggctga ttaacggtgc gccgaagtac ggcaacgacg
     3721 atgatactgt cgatacgctg ctggctcgcg cttatcagac ctatattgac gaactgaagc
     3781 agtaccataa cccgcgctac ggtcgtggtc cgattggcgg caactattat gcgggcacat
     3841 cgtctatctc cgctaacgta ccgtttggcg cgcagactat ggcaacgccg gacggacgta
     3901 aagcacatac cccgctggca gaaggcgcta gtccggcctc cggtactgac catctcggcc
     3961 ctactgcggt cattggctcg gtgggtaaac tgcctacggc cgcgattctc ggcggcgtgc
     4021 tgctcaacca gaaactgaat ccagcaacgc tggagaacga atctgacaag cagaaactga
     4081 tgatcctgct gcgcaccttc ttcgaggtgc ataaaggctg gcatattcag tacaacatcg
     4141 tttcccgcga aacgctgctg gaagcgaaaa aacatccgga tcagtaccgc gatctggtag
     4201 tgcgtgtcgc gggctattcc gccttcttca ccgcgctctc tccagacgct caggacgata
     4261 tcatcgcccg tactgaacat atgctgtaat ctcctcagcc cggcggcgtc gtcgccgggt
     4321 caaatcattc acatcatcaa ttccgctctc actttcattc gaaatataat ttgtgctctg
     4381 cgtcacattg ttattacact gttacggttt cattttcgag ccaggagcac acctatgagc
     4441 gtaaaagtta tcgtcacaga catggacggt acttttctta acgacgccaa aacgtacaac
     4501 caaccacgtt ttatggcgca gtatcaggaa cttaaaaagc gcggcattga gttcgttgtc
     4561 gccagcggta atcagtatta ccagcttatt tcgttctttc ctgagctaaa ggatgagatc
     4621 tcttttgtcg cggaaaacgg cgcactggtt tacgaacatg gcaagcagtt gttccacggc
     4681 gaactgaccc gacatgaatc gcggattgtt attggcgagt tgctaaaaga taagcaactc
     4741 aattttgtcg cctgcggtct gcaaagtgca tatgtcagcg aaaatgcccc cgaagcattc
     4801 gtcgcactga tggcaaaaca ctaccatcgc ctgaaacctg taaaagatta tcaggagatt
     4861 gacgacgtac tgttcaagtt ttcgctcaac ctgccggatg aacaaatccc gttagtgatc
     4921 gacaaactgc acatagcgct ggatggcatt atgaaacccg ttaccagtgg ttttggcttt
     4981 atcgacctga ttattcccgg tctacataaa gcaaacggta tttcgcggtt actgaaacgc
     5041 tgggatctgt cgccgcaaaa tgtggtagcg attggcgaca gcggtaacga tgcggagatg
     5101 ctgaaaatgg cgcgttattc ctttgcgatg agcaatgctg cggaaaacat taaacaaatc
     5161 gcccgttacg ctaccgatga taataatcat gaaggcgcgc tgaatgtgat tcagacggtg
     5221 ctggaaaata aaccgccgtt taatctttga ttatcgccaa ttcagtttat tgagaaagtg
     5281 catgtcatta tgtcggatac ggcgtaaccg ccttattcgg catacaaaat catgaaaatt
     5341 caatatattg cgatacacta ccaggcctga taagcgtagc gcaccaggca atcttgcgtt
     5401 tgtcatcagt ttcagtagcc ctgaccatat cagggctact gataataact tactcgatcc
     5461 ctttactacg caggtaatct tcgtaattac cgctaaagtc gatcacgcgt tccggggtga
     5521 tttccagaat acgggtcgcc agggagctta caaactcacg gtcgtgagaa acaaagatca
     5581 gcgtgccctg atacagctcc agcgccatgt tcagcgactc aatggattcc atatccaggt
     5641 ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg catcattaac ttaccaaaca
     5701 gcatccgccc tttttcccca ccggaaagca ctttcgctgg ctttttgatg tcgtcctggc
     5761 tgaacagtaa acgaccgaga atgctgcgta ccgcctgctc gtcatcgcct tcctgcttcc
     5821 actggctcat ccattcgaac acggtcagat cattttcgaa ctcatattca tggtcctggg
     5881 cgtagtaacc aatgcgcgcg ttctcagacc attttacggt gccgctgtcc ggttgcagat
     5941 cgcccaccag cgttttcagc agcgttgatt taccgacgcc gttggtaccc agtacagcca
     6001 gtttttcacc cacttccagc agcaggttga gatttttaaa cagtggaccg ttatcaaacc
     6061 ctttagtcag accttccact tccagcgcgt tacggaacag tttcttatcc tgttcaaaac
     6121 ggatgaacgg gttctgacgg ctggaggctt tcacctcttc cagtttgatt ttatcaatct
     6181 ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt ggcgctaaag cggctaacga
     6241 aagattgcaa ctcagcaatc tgcgctttct tcttggcgtt atcagccagc agacgttcac
     6301 gcgcctgagt cgccgccgtc atgtactcat cgtagttgcc cggataaacg cgcagctcgc
     6361 cgtaatccag atccgccatg tgggtacaaa ccatgttaag gaagtgacgg tcgtgcgaga
     6421 tgatgatcat ggtgctgtca cgctcgttca gcacctgttc cagccagcga atggtgtcga
     6481 tgtcgaggtt gttggtcggt tcgtcgagca ggagaatgtc cggatcagca aacagcgcct
     6541 gcgccagcag cacacgcagc ttccagccag gagcaacttc actcatcggg ccgtagtgct
     6601 gttccactgg aattcccacg ccaagcaaca gttcaccagc gcgagcttcc gcagagtaac
     6661 cgtccatttc gccgtatttc acttccagat cggccacttt atagccgtct tcttcactca
     6721 tttccggcaa agcatagatg cggtcacgtt cctgcttcac ttcccacaac tctttatgcc
     6781 ccatgatcac cgtatccagc acagtgaact cttcaaaggc aaactgatcc tgacgcagct
     6841 taccaatgcg ctcattggga tcgagggaaa cgttacccaa cgtcggctct aagtcgccgc
     6901 cgaggatctt cataaaggtg gatttaccac taccgttcgc gccaatcagg ccgtaacggt
     6961 tgccgccgcc gaatttgacg gaaatgtttt caaacaacgg cttactgccg aactgcatgg
     7021 tgacgttact ggaaactaac acaggcgtat cctgaaaaga gatatgacaa accgcgcatt
     7081 atgccataag tctgaaagaa gatcctgctc ctcgcttaag gtctacactc aagccaaagg
     7141 caaaaaatgt gatttcgtac acatctgatt tcactgtgag ctggaatgaa cttataatgc
     7201 gcttccaata ctctaatatt ctcaacccaa tggcctgcca ggcacaaaat ctcgcttaac
     7261 atgaatatga aattgaaaac attattcgca gcggccttcg ctgttgtcgg cttttgcagt
     7321 accgcctctg cggtaactta tcctctgcca accgacggga gtcgcctggt tggtcagaat
     7381 caggtgatca ccattcctga aggtaacact cagccgctgg agtattttgc cgccgagtac
     7441 cagatgggac tttccaatat gatggaagcg aacccgggtg tggatacctt cctgccgaaa
     7501 ggcggtactg tactgaacat tccgcagcag ctgatcctgc cggataccgt tcatgaaggc
     7561 atcgtcatta acagtgcaga gatgcgtctg tattactatc cgaaagggac caacaccgtt
     7621 atcgtgctgc cgatcggcat tggtcagtta ggcaaagaca cgcctatcaa ctggaccacc
     7681 aaagttgagc gtaaaaaagc aggcccgacc tggacgccga ccgctaaaat gcacgcagag
     7741 taccgcgctg cgggcgaacc gcttccggcg gtcgttccgg ctggtccgga taacccgatg
     7801 gggctgtatg cactctacat cggtcgcctg tacgctatcc atggcaccaa cgccaacttc
     7861 ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc gtaacgaaga catcaaattc
     7921 ctgttcgaga aagtaccggt cggtacccgc gtacagttta ttgatgagcc ggtaaaagcg
     7981 accaccgagc cagacggcag ccgttatatt gaagtccata acccgctgtc taccactgaa
     8041 gcccagtttg aaggtcagga aattgtgcca attaccctga cgaagagcgt gcagacagtg
     8101 accggtcagc cagatgttga ccaggttgtt ctggatgaag cgatcaaaaa tcgttccggg
     8161 atgccggttc gtctgaatta atcttcaaaa cgtaaagcaa aggcggactc ataatccgcc
     8221 tttttaattt gacagacctt atcccgccgg gatcataacc aacaaaacat atcccaccag
     8281 cgccgcccac aacagcatcg gtatcgaata aagatgaaaa cgccaccaga tacggcgatc
     8341 gttcgccatc cgtaacgcaa tcaaatttgc cagcgatccg ggtaataaac caaagccccc
     8401 cacgtttacc gcccatgcca gtaataaaga cggtggcaca taattgagca gcaaaatggt
     8461 actcggcacg ttactgatca cctgcgataa accgatcgcc gttaaccata atccgggttc
     8521 ggataaatga ctcacgttac ttaacacccc ctgcaaaact ggcagttggg ttagtaaatg
     8581 gacgtcgata aacatcgcca taaacaccag cagcagcgtc cagtccacac tgagcaccac
     8641 acgacgcgcc agcagcgcaa agcccgccgc cacaatcacc agtccccaca gctcttgttt
     8701 taactccagc gccgtcagaa agacgatata cagccccaga caactccaca ccagccgcgg
     8761 tttccactcc ggcgtttgca cccccgtatg gtattgcagc gcctttccag ggaaacaaca
     8821 ccagcacaac agcaggagtg tcagcatcat tgcgccagcc agcggtgcca tttgggcaat
     8881 aaatccggca aacgaaagac cagaacgtcc ccagataaga atattttgcg ggttgccaat
     8941 tggcgtcagt agcgaaccag cgttgactgc cagcgcctca aaaataatca gccgattaac
     9001 cggaatctca cacagtcttt ttagcgtgat agtcagcgga acaactataa acagtgcgac
     9061 atcgttggtc agaaaggtag aaagcagcgc tgccgccagt accataaaca tcgccagccg
     9121 acgctccgta gcaaaacggc gcaccatttt gcgccccagc acatcaaaat aaccgcttaa
     9181 ctcgacacct ttggtcagca gcatcaggcc gcttaaggtg atgatggtgt gccagtcgat
     9241 agcagcaggc caggatttcg gtgcaaaggg aacgaaaaaa cttaatccga tgccaacaag
     9301 aattaataac tgaaaaaaac gatcgccttg cagcgtgcgt aaaaaaggca ggctcatttg
     9361 gcaccgtgtt tctgggtaaa caaacgaaag gcatccagcg tctcttcact aacatggtgc
     9421 tccatacctt ccgcgtcgcg acgggcgatt tccggactga cgcccaacac caacaagaaa
     9481 ttttcgacta tctgatgtcg ctcgcggctt tcctgcgcca gcttctctcc ttctgccgtt
     9541 aaaaacacgc cacgccaggg gatcatttca atcagcccca tggttgccag tcgcttaagc
     9601 attttagcca ccgtcggttg cgaaaccccc agacgagcag ccatgtccac ctgacgagct
     9661 tcccccactt ccctgatcaa gtcagaaatc agctcaacgt aatcatcaat cagctcgcgc
     9721 cgatgcgcct ctctgacctg gcggaaccct tcaacgtgct cttccacgtt cactaactgc
     9781 gtcacttttt ttgctgttgg cgtacctgcg cgacgactca ttgtgcttcc tcattatggt
     9841 gacgcataca tagcgttctc atttcgtgcg cacattgtaa accagagttg cgaaggtaca
     9901 aaaaattaac gttttagcaa tagctatata atatagcctg tgctatatct gtatgtaatg
     9961 caatcatccc tcaaggatcg atgggattag caagtcagga ggtcttatga atgagttcaa
    10021 gaggtgtatg cgcgtgttta gtcattctcc ctttaaagta cggttaatgc tgctctctat
    10081 gttgtgcgat atggtcaaca acaaaccgca gcaggataaa ccttccgata aatagcggcg
    10141 tcgcggtacg ccgcttcact cctgctttta tgcaggcaca acgcgttttg gtctgaaaaa
    10201 ccccactttt tgtcggattt gcaatcccct tcgcaaaaga tttgttcgtc ggtagttgac
    10261 ctgaacagca gctcgctcta tcttcttgca gccctgcgta tattgcggct cgcggatgcg
    10321 gacccctttc ccctcttcac gcactcttgc aggtattgac ccttgacgcc agggtaagca
    10381 catggcgttt gttacgatag tggcatatga atttaaccct caaagaatcg cttgttaccc
    10441 gtagccgggt atttagcccg tggactgcgt tctacttttt acagtcgcta ttaattaacc
    10501 tcggcttagg ttaccccttc agtttgctct acaccgctgc gtttacggct attttgcttt
    10561 tgctatggcg aacattgcct cgcgtacaaa aagttctggt cggtatcagt tcgctggtgg
    10621 cggcttgtta tttccctttt gctcaggcct acggcgcgcc taacttcaat acattgctgg
    10681 cattgcactc caccaatatg gaagagtcga ccgaaatcct gacgattttt ccgtggtaca
    10741 gctacctggt cggcatattt atttttgcgc tcggcgtaat agcaatcagg cgaaaaaaag
    10801 agagtgaaaa agcgcgctgg aataccttcg acagcctgtg cctggtattc agtgtggcga
    10861 cattttttgt tgctcccgtg caaaacctgg cctggggcgg cgtatttaaa ctgaaagata
    10921 ctggctatcc ggtatttcgt tttgctaagg atgtcatcgt caataataac gaggtgattg
    10981 aagagcaaga acggatggca aaactttccg gaatgaaaga tacctggacg gtcactgccg
    11041 ttaagccgaa gtatcagacc tatgtggtgg tgatcggaga aagcgcgcgt cgcgatgccc
    11101 tcggtgcctt tggcggtcac tgggacaata ccccgtttgc cagcagcgtt aacggtttga
    11161 tatttgctga ctacattgcc gccagtggct ccacgcagaa atcgcttggc ttaacgctca
    11221 atcgcgttgt cgatggcaaa ccacagtttc aggataactt tgtcaccctg gcaaatcgcg
    11281 cgggcttcca gacctggtgg ttttccaacc agggccaaat cggcgaatac gataccgcca
    11341 tcgccagcat cgccaaacga gcagatgaag tgtacttcct gaaagaaggt aattttgaag
    11401 cagataaaaa cacgaaagac gaagcgttac tggatatgac cgctcaagtg ctggcgcaag
    11461 agcactcgca acctcagctg attgttctac atctgatggg ctcgcatccg caggcctgcg
    11521 accggacaca aggaaaatac gaaacctttg tgcaatcgaa agaaacgtcg tgctatctct
    11581 ataccatgac gcaaacagac gatttactgc gcaagctgta cgatcagtta cgcaacagcg
    11641 gcagcagctt ctcgctggtt tacttttctg accacggtct ggcctttaaa gagcgcggca
    11701 aagacgtgca ataccttgcc catgatgata agtatcagca aaatttccag gtgcctttta
    11761 tggtcatttc cagcgacgat aaagcgcatc gggtgattaa agcccgccgc tcggccaatg
    11821 acttcttagg ctttttctcg cagtggacgg gaattaaagc gaaggaaata aatatcaaat
    11881 acccgtttat atctgagaag aaagccgggc cgatatacat caccaacttc cagttacaga
    11941 aggtggatta caaccatctc ggaaccgata ttttcgaccc gaaaccttaa aacaaaaaaa
    12001 tccgccccga gaggcggatt ttttatatca ccgaagtgat tagaagcggt aaccaacacc
    12061 ggcaatccag gtgcctacgt caacgctacg aatacggctc tgctcgtaag agaagtccag
    12121 agcaacgttt tccatcgggt tgaactgcag accagcaccg taggagaaac cgtagtcgct
    12181 ggtgtcgtgt ttgtaggtcg ggtattcagt ggtctggaat ttaccataac ccacacccac
    12241 tacaccgtag atgcttgccc agtcgttaat gcggtaagcc ggaccagcag tgatgccgta
    12301 gtactggttt ttgttgtagt caccagagct tgcagtacgg cttttctcgg tgtaagtgaa
    12361 agaaccgatc acacccagcg ggctgttgtc ttcttcatag cggtatttca ggttgaaacc
    12421 gcccattttg ttcatttggc cctgagcgtc gctctgtgcg taaccgccag ttacagtaga
    12481 agtcgcagct acggaagtac ctgcggtgaa agccagaact gcggccagtg ctgaaagaca
    12541 tgcaattttt ttcataacca cctcaaatgt gattcaaata agtcctaagt tttaaatata
    12601 tcaaaaatta atgggaaact cttcgcgatt tatgatgtct aacgggccat ttcatgtaac
    12661 agaacgtttc catacaccgc tatccatcta aatttaaatc actttttcag agaactgcgt
    12721 aagtattacg catgttttcc ctgtcattca tccagattat tcctaatcac cagactaatg
    12781 attccatcaa tcctggcgca ttttagtcaa aacgggggaa aattttttca acaaatgctc
    12841 aaccagcatt gggtatatcc agtacactcc acgctttact taggtttaga tatttgtggg
    12901 agaaaggatg cctggttcat tacgtaaaat gccggtctgg ttaccaatag tcatattgct
    12961 cgttgccatg gcgtctattc agggtggagc ctcgttagct aagtcacttt ttcctctggt
    13021 gggcgcaccg ggggtcactg cgctgcgtct ggcattaggc acgctgatcc tcatcgcgtt
    13081 ctttaagcca tggcgactgc gctttgccaa agagcaacga ttaccgctgt tgttttacgg
    13141 cgtttcgctg ggtggaatga attatctttt ttatctttct attcagacgg taccgctggg
    13201 tattgcggtg gcgctggagt tcaccggacc actggcggtg gcactgttct cttctcgtcg
    13261 cccggtagat ttcgtctggg ttgtgctggc ggttcttggt ctgtggttcc tgctaccgct
    13321 ggggcaagac gtttcccatg tcgatttaac cggctgtgcg ctggcactgg gggccggggc
    13381 ttgttgggct atttacattt taagtgggca acgtgctgga gcggaacatg gccctgcgac
    13441 ggtggcaatt ggttcgttga ttgcagcgtt aattttcgtg ccaattggaa cgcttcaggc
    13501 tggtgaagca ctctggcact ggtcggttat tccattgggt ctggctgtcg ctattctctc
    13561 gaccgctctg ccttattcgc tggaaatgat tgccctcacc cgtttgccaa cacgcacatt
    13621 tggtacgcta atgagcatgg aaccggcgct ggctgccgtt tccgggatga ttttcctcgg
    13681 agaaacactg acacccatac agctgctggc gctcggcgct atcatcgccg cttcaatggg
    13741 ttctacgctg acagtacgca aagagagcaa aataaaagaa ttagacataa attaaattta
    13801 catttcagca tggttatgca taaccatgca gattttctcg ctacttttcc tctacaccgt
    13861 ctttatatat cgaattatgc aaaagcatat ttattccgaa aattcctggc gagcaaataa
    13921 ataagaattg ttcttattaa tatatctaac tcattgagtt tttattggtt ttgttttttc
    13981 acgcttgtta ccactattag tgtgatagga acagccagaa tagcggaaca catagctggt
    14041 gctatactta gtctcgttaa ttactgggac ataacatcaa gaggatatga aattatgagt
    14101 accgctaaat tagttaaatc aaaagcgacc aatctgcttt atacccgcaa cgatgtctct
    14161 gacagcgaga aaaaagcaac agtagagttg ctgaatcgcc aggttatcca gtttattgat
    14221 ctttctttga ttaccaaaca agcgcactgg aacatgcgcg gcgctaactt cattgccgta
    14281 catgaaatgc tggatggctt ccgcactgca ctgatcgatc atctggatac catggcagaa
    14341 cgtgcagtgc agctgggcgg tgtagctctg gggaccactc aagttatcaa cagcaaaact
    14401 ccgctgaaaa gttacccgct ggacatccac aacgttcagg atcacctgaa agagctggct
    14461 gaccgttacg caatcgtcgc taatgacgta cgcaaagcga ttggcgaagc gaaagatgac
    14521 gacaccgcag atatcctgac cgccgcgtct cgcgacctgg ataaattcct gtggtttatc
    14581 gagtctaaca tcgaataaat ccatcgctga tggtgcagaa ctttagtaca cgataaaagc
    14641 ggcttcctga caggaggccg ttttgttttg tagcccgcct caacgcactt atttagtgca
    14701 tccatctgct atcaccagct ggttaagtaa attttttgta tccacatcat cacacaatcg
    14761 ttacataaag attgtttttt catcaggttt tacgctaaat aatcactgtg ttgagtgcac
    14821 aattttagcg caccagtttg gtgccccaga atggtgcatc ttcagggtat tgccctataa
    14881 atcgtgcatc acgtttttgc cgcatctcga aaaatcaatg agttgcaaaa ctggcacgat
    14941 tttttcatat atgtgaatgt cacgcagggg atcgtcccgt ggatagaaaa aaggaaatgc
    15001 tatgaagtct gtattaaaag tttcactggc tgcactgacc ctggcttttg cggtttcttc
    15061 tcatgccgcg gataaaaaat tagttgtcgc gacggatact gccttcgttc cgtttgaatt
    15121 taaacagggc gataaatacg tgggctttga cgttgatctg tgggctgcca tcgctaaaga
    15181 actgaagctg gattacgaac tgaagccgat ggatttcagt gggatcattc cggcactgca
    15241 aaccaaaaac gtcgatctgg cgctggcggg cattaccatc accgacgagc gtaaaaaagc
    15301 gatcgatttc tctgacggct actacaaaag cggcctgtta gtgatggtga aagctaacaa
    15361 taacgatgtg aaaagcgtga aagatctcga cgggaaagtg gttgctgtga agagcggcac
    15421 tggctccgtt gattacgcga aagcaaacat caaaactaaa gatctgcgtc agttcccgaa
    15481 catcgataac gcctatatgg aactgggcac caaccgcgca gacgccgttc tgcacgatac
    15541 gccaaacatt ctgtacttca tcaaaaccgc cggtaacggt cagttcaaag cggtaggtga
    15601 ctctctggaa gcacagcaat acggtattgc gttcccgaaa ggtagcgacg agctgcgtga
    15661 caaagtcaac ggcgcgttga aaaccctgcg cgagaacgga acttacaacg aaatctacaa
    15721 aaaatggttc ggtactgaac cgaaataata acgcggcgcc tgtaaagcgc actggcagtt
    15781 ccctctcccc gccggggaga ggattagggt gaagggcgca aacccgctcc ggggccatta
    15841 attaccctga atttgattat ttacaccacg gtaacaggaa caacatatgc agtttgactg
    15901 gagtgccatc tggcctgcca ttccgcttct gattgaaggt gccaaaatga ccctgtggat
    15961 ttcggtcctc ggtctggctg gcggtctggt gatcggattg ttggcaggtt ttgcacgcac
    16021 cttcggtggt tggatagcca accacgtcgc gctggtcttt attgaagtga tccgcggcac
    16081 acctatcgtc gtccaggtga tgtttatcta tttcgccctg ccgatggcgt ttaacgattt
    16141 acgcatcgac ccgtttactg cggcggtggt caccatcatg atcaactccg gcgcgtacat
    16201 tgcggaaatc acgcgtggtg cggtgctgtc tattcacaaa ggttttcgtg aagcaggact
    16261 ggcgctcggt ctttcacgtt gggaaaccat tcgctacgtc attttaccgc tggcactgcg
    16321 tcgtatgctg ccgccgctgg gtaaccagtg gatcatcagc attaaagaca cttcgctgtt
    16381 tattgttatc ggcgtggctg aactgacccg tcaggggcaa gaaattattg ccggtaactt
    16441 ccgcgccctt gagatctgga gcgccgtggc ggtgttctat ctgattatta ccctggtgct
    16501 gagctttatt ctgcgtcgtc tggaaagaag gatgaaaatc ctgtgattga atttaaaaac
    16561 gtctccaagc attttggtcc aacccaggtg ctgcacaata tcgatttgaa cattgctcag
    16621 ggcgaagtcg tggtgattat cgggccgtcc ggttccggta aatcgaccct gctgcgctgc
    16681 atcaacaaac tggaagaaat cacctccggc gatctgattg tcgatggcct gaaggttaac
    16741 gatccgaaag ttgatgagcg cctgattcgc caggaagcgg gtatggtgtt ccagcagttt
    16801 tacctcttcc cgcatctgac ggcgctggaa aacgtcatgt ttggcccgct acgcgtgcgt
    16861 ggcgcgaata aagaagaggc ggaaaaactg gcgcgtgagc tgctggcgaa agtcggtctg
    16921 gcagagcgtg cacatcacta cccttccgaa ctttctggtg gtcaacagca gcgtgtggcg
    16981 attgcccgcg cgctggcggt gaagccgaaa atgatgctgt ttgatgaacc gacttccgct
    17041 cttgacccgg aactgcgcca tgaagtgctg aaggttatgc aggatctggc tgaagaaggg
    17101 atgacgatgg tgatcgtgac ccacgaaatc ggttttgccg agaaagtagc ttcgcgcctg
    17161 atctttatcg acaaaggccg gattgcagaa gatggcgatc cgcaggtgtt gatcaagaac
    17221 ccgccgagcc agcgcttgca ggaatttttg cagcacgtct cttaacttct ctgcctccct
    17281 ttcccccgaa agggaggcac accagattcc tctcatttaa aatcgcccag cctccagcat
    17341 ctatacttat ctttttgctc tgttttctca ctggaggagt catgcggtgg atcctgttca
    17401 tcctcttctg cctgttgggc gcacctgccc acgcggtatc catacccggc attacaacca
    17461 caacgacaac ggactcaacg actgaaccgg ccccggaacc ggatatcgaa caaaaaaaag
    17521 cggcctatgg cgcactggcg gatgtgctgg ataatgacac ctcgcgtaaa gagttgatcg
    17581 accagttgcg caccgtcgcc gcgacgcccc ctgcggaacc cgtaccgaag atcgtgccgc
    17641 cgacgctggt tgaagagcaa accgtgctgc aaaaagtcac cgaagtcagc cgccattatg
    17701 gtgaagccct ttccgcccgc ttcggacaac tttatcgcaa tatcaccggc tccccgcata
    17761 agccgtttaa tccacaaacc ttcagcaatg cgttgaccca tttttcaatg ttagcggtat
    17821 tagtgtttgg tttttactgg ctgattcgcc tgtgcgcact tccgctgtat cgcaaaatgg
    17881 gccagtgggc gcggcaaaaa aatcgtgagc gcagtaactg gttgcagctt ccggcgatga
    17941 ttatcggggc gtttattatc gacctgctgt tactggcact gacattattt gtcggtcagg
    18001 ttttaagcga caacctgaat gcgggcagtc gcaccatcgc tttccaacaa agtttgtttc
    18061 tcaacgcctt tgccctgatt gaatttttca aagccgtact acgcctgatt ttttgcccaa
    18121 acgtggctga actgcgcccg ttcacgattc atgacgagac cgcccgttac tggagtcgtc
    18181 gcctgagctg gttaagcagc ctgattggct atggtctgat tgtggctgtg ccgattatct
    18241 ctaatcaggt gaatgtacag ataggtgcgc tggcgaacgt catcattatg ctgtgcatga
    18301 ccgtctgggc gttgtacctg atttttcgta ataaaaaaga gatcacccag catttgctca
    18361 actttgcgga gcattcgctg gcctttttca gcctgtttat ccgcgccttt gcgctggtgt
    18421 ggcactggct ggcaagcgcc tattttatcg tgctgttttt cttttcgttg ttcgatccgg
    18481 gcaacagcct gaaatttatg atgggtgcaa cggtgcgcag cctggcgatt attggtatcg
    18541 cagcatttgt ttccggtatg ttttcccgct ggttggcgaa aaccatcacc ctctcgccac
    18601 atactcagcg taactatccc gagctgcaaa aacggctgaa tggatggctt tcggcggcac
    18661 tgaaaacagc gcgtattctg acagtctgcg tggcggtaat gctgctgttg agcgcatggg
    18721 gattgttcga tttctggaac tggctgcaaa acggagcggg gcagaaaacc gtagatatcc
    18781 tgatccgtat cgcactcatt cttttcttct cggcggttgg ctggacagtg ctcgccagtt
    18841 tgatagaaaa ccggctggct tcggatattc atggccgccc gctacccagc gcccgtacgc
    18901 gaaccctgct gacgctgttc cgtaacgcgc tggcggtgat tatcagtacc atcaccatca
    18961 tgattgtgtt gtcggaaatc ggcgtcaata tcgcgccatt gctggcaggt gccggggcat
    19021 taggtctggc tatctcgttt ggttcgcaaa cgctggtgaa agatattatt accggagtat
    19081 ttattcagtt tgaaaacggc atgaacactg gagatttggt gactatcggg ccgttgaccg
    19141 gcactgtgga acggatgtcg attcgctccg tgggcgtgcg ccaggatacc ggggcgtatc
    19201 acatcattcc gtggtcttcg ataaccacct ttgctaactt cgtccgcggt attggttcgg
    19261 tagtggcaaa ctacgatgtt gatcgccatg aagatgctga taaagccaat caggcactga
    19321 aagatgcggt agcagaattg atggaaaacg aagaaattcg cgggctggtt attggtgaac
    19381 cgaactttgc cgggattgtc ggcttaagca ataccgcgtt tacactgcgt gtttcgttca
    19441 ccacgctgcc actcaaacag tggacggtcc gctttgccct cgacagccag gtgaaaaaac
    19501 atttcgacct ggcgggtgtt cgcgcgccgg tgcagactta tcaggtgctg ccagctccgg
    19561 gcgcggcccc ggctgaaccg ttgccgccgg gggagccaac gctttaacgc tggcgattga
    19621 caaaacggcg gcgctgctcg tcgttcataa aggtccaggc tataaagcgg ctctgctttt
    19681 gcccctgggc catctctttt tttaccacct tcaccgcacc tacgtccgtc agagcacgat
    19741 acaacggcgg taagttttca ccacgagata ccagcgaggt aaaccacatc acctgcttcg
    19801 cgaagccttt gctctcttca atcatctttt tgataaaagc gacctcaccg ccttcacacc
    19861 acaactcctg ttgctgacca ccaaagttca gtgcatcgtc tttgttcagc cccaggttac
    19921 ggcgtttacg ttcactacct gcccgtgccg cagcggcgga atcgtggaat ggcgggttac
    19981 acaaggtcgc atcgtattgc tcgtttttat ggatgatgcc gttaaaaatc gccccactct
    20041 ctttttgccg acgcagacga atagcgcggt taagccccgg attagcactg atgatcgcct
    20101 gcgcactgct taacgcctgg ctgctggttt cactaccggt aaaacgccag ccatattcat
    20161 gtacgccaat cagcggataa atacagttcg caccaacgcc gatatccaga atgctggcat
    20221 tagctggaat tgttccgctt gcttcagcca gtaaatcggc aaggtgatga atataatccg
    20281 cccggcccgg tactggcggg cagagaaaac cgtccgggat atcccagttc gctacggcgt
    20341 aaaaatgggc cagcaacgcc ttattgagcg ccttcaccgc cagcggattg gcaaagtcta
    20401 cgctttgctc cccggcgggt gtaagcgtga ggaattgcct gagttcagga ttgacctgac
    20461 aaagcgtggc gagatcgtag cggctgtgat gacggttgcg cggatgcaac cccggtttct
    20521 gggcggacat agcattctcc tttaagcagc ggcgtaagat acccgcataa cgcgacaggg
    20581 taaatatctg gctgcgttta tcatttctaa aaaccttcac atggctatgg tttatggcaa
    20641 ttcaccataa aaatcatcat catgcatgtc attgatttgt cttattttgt aaaaatatat
    20701 cgttcaaaaa ataagcgtat ctctgtcaat tttttgcgtc attttcatcg cgcaggcgac
    20761 ctacacttat ctggcactac ccgttgtatt tatgaggatg tcattatgaa aaagtgcctc
    20821 acactactga ttgccaccgt cctgagcgga atctctctca cggcttatgc cgcgcaaccg
    20881 atgagtaacc tggacagtgg tcaactacgg cccgccggta ccgtttcggc aactggcgca
    20941 tcaaacctaa gcgatctgga ggataaactg gcagaaaaag cgcgcgggta atgactccaa
    21001 cttattgata gtgttttatg ttcagataat gcccgatgac tttgtcatgc agctccacc
//
LOCUS       DAZFFE010000084        20180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFFE010000084 DAZFFE010000000
VERSION     DAZFFE010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.084"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1169
                     /locus_tag="ACHJ69_003360"
     CDS             <1..1169
                     /locus_tag="ACHJ69_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO5016281.1"
                     /translation="FAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIM
                     IAFLVARNVAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAV
                     IVALITSEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLI
                     SPAGLNDLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSE
                     ANLENLSWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRD
                     LAKMSFIPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAY
                     AVPWTTPGPLIAFLGTGGNWLALLVGFLCLGVATIIYLPFVIAANKVNNMTTNG"
     gene            1197..1508
                     /locus_tag="ACHJ69_003361"
     CDS             1197..1508
                     /locus_tag="ACHJ69_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFO5016282.1"
                     /translation="MFADEELVMELLINAGQARSDAMEAIRCAGQKDWQGATKLMASS
                     ESACLQAHKIQTALISQDEGCGKIEVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            1563..3236
                     /locus_tag="ACHJ69_003362"
     CDS             1563..3236
                     /locus_tag="ACHJ69_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO5016283.1"
                     /translation="MNIKTLNVSLLSISIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKFRLSTLQKSITENKYQSASLAEISATSPVADNIKNENGEQNS
                     SAAAHTINGSQQIAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGRTANAVVTYDGNVGQQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAREAEFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFTRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDDSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            3260..4699
                     /locus_tag="ACHJ69_003363"
     CDS             3260..4699
                     /locus_tag="ACHJ69_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO5016284.1"
                     /translation="MSGFKEDFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAGMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIHFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDLNEREQIMYQAVHYEL
                     VASALAVQTGKLINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPDDNAILARGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFSSHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(4756..4974)
                     /gene="marB"
                     /locus_tag="ACHJ69_003364"
     CDS             complement(4756..4974)
                     /gene="marB"
                     /locus_tag="ACHJ69_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO5016285.1"
                     /translation="MKPLLSAIATALILFSAQGVAEQTTQPVVTSCGNVVVVPTSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(5006..5389)
                     /gene="marA"
                     /locus_tag="ACHJ69_003365"
     CDS             complement(5006..5389)
                     /gene="marA"
                     /locus_tag="ACHJ69_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO5016286.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNN"
     gene            complement(5410..5844)
                     /gene="marR"
                     /locus_tag="ACHJ69_003366"
     CDS             complement(5410..5844)
                     /gene="marR"
                     /locus_tag="ACHJ69_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO5016287.1"
                     /translation="MKNTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            6056..6721
                     /gene="marC"
                     /locus_tag="ACHJ69_003367"
     CDS             6056..6721
                     /gene="marC"
                     /locus_tag="ACHJ69_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO5016288.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMSSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(6746..7936)
                     /gene="ydeA"
                     /locus_tag="ACHJ69_003368"
     CDS             complement(6746..7936)
                     /gene="ydeA"
                     /locus_tag="ACHJ69_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO5016289.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAHSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGMLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWSVTLE
                     EQTQ"
     gene            complement(8078..8197)
                     /gene="yneK"
                     /locus_tag="ACHJ69_003369"
     CDS             complement(8078..8197)
                     /gene="yneK"
                     /locus_tag="ACHJ69_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO5016290.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEEQAPFLIVIKM"
     gene            complement(8275..9156)
                     /locus_tag="ACHJ69_003370"
     CDS             complement(8275..9156)
                     /locus_tag="ACHJ69_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5016291.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILTLVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDAPDSAKQGYQ"
     gene            9257..10645
                     /gene="sad"
                     /locus_tag="ACHJ69_003371"
     CDS             9257..10645
                     /gene="sad"
                     /locus_tag="ACHJ69_003371"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO5016292.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGVDDIENALQLAAAGFRD
                     WREANIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSLMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRV"
     gene            10709..11635
                     /gene="glsB"
                     /locus_tag="ACHJ69_003372"
     CDS             10709..11635
                     /gene="glsB"
                     /locus_tag="ACHJ69_003372"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO5016293.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            11635..11994
                     /locus_tag="ACHJ69_003373"
     CDS             11635..11994
                     /locus_tag="ACHJ69_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO5016294.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGEALSEEQQR
                     YIVTVIYHWLVIQMNQP"
     gene            12133..13551
                     /locus_tag="ACHJ69_003374"
     CDS             12133..13551
                     /locus_tag="ACHJ69_003374"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO5016295.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFVLTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVARDKRLGKNKYEPVSALRLFNYCLLAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEVVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            13778..15229
                     /gene="uxaB"
                     /locus_tag="ACHJ69_003375"
     CDS             13778..15229
                     /gene="uxaB"
                     /locus_tag="ACHJ69_003375"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO5016296.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQYRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            15426..16340
                     /locus_tag="ACHJ69_003376"
     CDS             15426..16340
                     /locus_tag="ACHJ69_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO5016297.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANHIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(16344..17102)
                     /gene="tam"
                     /locus_tag="ACHJ69_003377"
     CDS             complement(16344..17102)
                     /gene="tam"
                     /locus_tag="ACHJ69_003377"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFO5016298.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYIADLGCGPGN
                     STALLDQRWPAARITGIDSSPAMIAEARSALPDCLFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLSPLGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            17410..17676
                     /gene="hipB"
                     /locus_tag="ACHJ69_003378"
     CDS             17410..17676
                     /gene="hipB"
                     /locus_tag="ACHJ69_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO5016299.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEAAEQQDLEW"
     gene            17676..18690
                     /locus_tag="ACHJ69_003379"
                     /pseudo
     CDS             17676..18690
                     /locus_tag="ACHJ69_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
     gene            complement(19065..19526)
                     /locus_tag="ACHJ69_003380"
     CDS             complement(19065..19526)
                     /locus_tag="ACHJ69_003380"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5016300.1"
                     /translation="MAVDKECAERIRLSIIQNFTYDDIENETGISVSTLKRLASGERE
                     PKLIEIRKIAKATGKNPVWLAFGESTLPLPENLGEDMTAFGYGIDFKLQGHETGEAAA
                     KIRLMAAIRDIEKDDIVFLEKFIDLLNLDRTINNVKTKRYGHTEEEARFKK"
     gene            19729..>20180
                     /locus_tag="ACHJ69_003381"
     CDS             19729..>20180
                     /locus_tag="ACHJ69_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO5016301.1"
                     /translation="MTESAILTAYNAIDAASVALGRPCLTLPEREHFRTERDVATRNL
                     ATLSRQYRDEVGTLSAQLALPRNTQVDRVIYGINCTDLSLADRGRAAGLVSQFGSFSF
                     TVAHAFCRLVYQLGVKNSLMALESAHRFAFFNQDGDPYEFSLFCTDEQ"
BASE COUNT         5131 a         4615 c         5006 g         5428 t
ORIGIN      
        1 actttgccat attatcgccc gacggtttta ttgccggatt actgcatctg aacagcattt
       61 tccccaacct tgaaaaagca caagctattt ttactccggt aatgaatggt tctgtaaata
      121 tcatgtcaat tatgattgct ttcctggtcg ccaggaatgt ggcgattagc tatgagcaag
      181 atgatctttt atgcggatta acggcaatag gagcattttt tattgtttat accccatacc
      241 agatgataga tgggcaggca ttcctgacga ccaaatatct cggcgcgcag gggttgtttg
      301 ttgctgttat cgttgcattg atcaccagtg aaatattttg tcgcttagct cgaaacccca
      361 aaatcaccat aacgatgccg gcagctgtac ctcctgcggt agcgcgttca tttaaagttt
      421 tattgccaat attttttgtt atggtgttct tttccgcact taattattgc ctgacattga
      481 tatccccggc aggattaaat gatctcattt acacattaat ccagacgccg ctcaaacata
      541 tgggaacgaa tatctttgcg gtaattatcc tgggggctgt gggtaatttc ctctgggtgc
      601 tggggatcca cggacctaat acaacgtcgg caattcgaga aaccgttttt tctgaggcta
      661 atctggagaa tctctcctgg gccgctcaac acggcactac ctggggcgcg ccatatccga
      721 ttacctggac ttctattaat gatgcattcg ccaactgcgg cggttcaggt atgacgttgg
      781 ggttattgtt ggctattttt atcgcttcta agcgtgcgga ataccgtgat ctggcaaaaa
      841 tgtcatttat ccccggtatt ttcaatatca atgaaccgat aatgttcggc cttcctattg
      901 tacttaaccc catcatgatg gtgccgttta ttatggttcc cattgttaac tgtgccattg
      961 gttacttctt tgtttcgatg gaaattattc caccggttgc ttatgccgtg ccctggacta
     1021 cgcccggacc tttaattgct ttcctcggaa ccgggggaaa ctggctggca ttactggttg
     1081 gttttttatg tttaggtgtg gcgacaatca tctatttacc ttttgttatc gccgccaaca
     1141 aagtcaataa catgacaact aacggataat tccatttacc caggaaggag attgtgatgt
     1201 ttgcagatga agaacttgtt atggagttac tgatcaacgc gggacaggct cgttctgatg
     1261 cgatggaagc catacgttgt gccgggcaaa aagactggca gggcgcaact aaactaatgg
     1321 ccagctctga atctgcctgt cttcaggcgc ataagattca gaccgcgtta attagtcagg
     1381 atgaaggctg tggcaaaata gaagttaatt taattctgat acatgcacag gatcatttga
     1441 tgaatgcaat attatgccag gatttagcca gggaaattat ttcattaaga aaagagctcc
     1501 atgcctgatt tttcctccag tgcgatatct ggaggaaatt actacactct taaggataag
     1561 tcatgaatat aaagacgctt aacgtcagcc ttttgtctat ttctattatt acagcattgt
     1621 ttccgttgaa cgcgatggca acaaaattaa ccatagagca gcgccttgaa ttgcttgaaa
     1681 atgaattgtc gcaaaataaa caagagctga aagcaacaca gaatgaactg ggagtatata
     1741 aattccgact ttcgacatta caaaaaagca tcacagaaaa taaatatcaa tcggcctcgc
     1801 ttgccgaaat atcagcgaca tctcccgttg ctgataacat caaaaatgaa aacggtgaac
     1861 agaactcgtc tgccgcagca catactataa atggatcgca gcaaattgcc gttattgaaa
     1921 gtaaaggcga taaaaccact atcgaaagcg tgaccctgaa agatatcagt aaatatataa
     1981 aggatgatat tggattcagc tatcaggggt acttccgctc aggctggggc accggtaatc
     2041 acggctcacc acaaacttat gcagcgggtt ctctgggacg ttttggtaac gagatgagtg
     2101 gttggtttga cctgacctta aatcagcgtg tttataatca ggacggtaga acggcaaatg
     2161 cggtcgttac ctatgatgga aacgtaggtc agcagtataa cgatgcctgg tttggtgaca
     2221 gtgccaatga aaatatcatg cagttcagtg atatttatct gacaacgcga ggttttttac
     2281 ccttcgcacg agaggcagaa ttctgggtcg gcaaacataa actcccgcaa tatgagatcc
     2341 aaatgctgga ctggaaaacc ttaaccacgg atgttgccgc gggtgtgggg attgaaaact
     2401 gggcgcttgg tgtagggctg tttgatatgt ccttaagccg agatgatgtc gatgtttact
     2461 ctcgtgattt tacgcgtacc agtcagatga ataccaattc tgtggatgtt cgttatcgca
     2521 atatcccgtt atgggatgat gcgacgttgt cattaatggc taaatattcc gcacctaata
     2581 aaacggatca acaacaagat aatgaaaatg acgacagtta ttttgaaatg aaagatagct
     2641 ggatgctgac ttctgtttta cggcaaaact tgcaacgcga tacgtttaat gaatttacgt
     2701 tacaggttgc caataattcc tatgccagca gttttgccag tttctcagat gccagtaata
     2761 cgatggcgca tggtcgctat tactatggtg accataccaa tgggatcgcc tggcgtttaa
     2821 tctctcaggg cgagatgtat ctgactgaca atattattat ggctaacgcg cttgtctatt
     2881 ctcatggcga agatgtttat agttatgaaa gtggcgctca tagtgatttt gacagtattc
     2941 gcaccgtaat aagaccggcc tggatctgga atacatggaa tcagacgggg cttgaattag
     3001 gctggtttaa gcaacagaac aaaactcagc agggggtaac gctaaatgaa tcggcttata
     3061 aaacgacact ctggcatgca ttgaaagtgg gtgaaagtat tttaggttca cgaccagaaa
     3121 ttcgcttcta cgggacgtat atcaatattc tggataacga attatcgaat tttaagttta
     3181 atgagaacag caaagacgag tttatggccg gcatccaggc ggaagtctgg tggtaatcta
     3241 tttagttaag gagttgtgta tgtcaggatt taaagaagat tttttatggg gcggtgcggt
     3301 agccgcacat caattggaag gtggctggaa tgaaggagga aaaggcatca gtatcgctga
     3361 tgtaatgact gctggcgctc acggggtgcc gcgtgaagtg acagaaggcg ttatcgacgg
     3421 acttaattat cccaatcatg aagcaattga tttttatcat cgctataaaa cagatattca
     3481 gttatttgcc gggatgggat tcaaatgctt tcgaacttcc attgcctgga cacgaatctt
     3541 tccgcaaggt gacgaacagg agccgaatga agagggttta caattttatg atgatctctt
     3601 cgatgaatgc ctgaagcagg gaatggaacc tgtggtgacg ctttcgcatt ttgagatgcc
     3661 ttatcatctg gtgacaaaat atggtggctg gcgaaaccgt aaactgatcg acttttttat
     3721 ccacttcgca tcaacggtct tcacgcgcta taaagaaaaa gtaaagtact ggatgacgtt
     3781 taacgaaatc aataatcagg tgaatttcag cgaaagcctg tgtccattta ctaattccgg
     3841 tatcttgtat tcgccagagg aagatctcaa tgagcgcgaa caaataatgt accaggcggt
     3901 acattatgag ttagttgcca gtgccctagc ggtacagact ggaaaactga tcaatcctga
     3961 atttaatatc ggctgtatga ttgctatgtg ccccatctat cctctgacgt gtgcacccaa
     4021 cgatatgatg atggccacga aagcgatgca tcgtcgttac tggtttactg atgttcatgc
     4081 tcgtggatat tatccgcaac atatgctgaa ttactttgca aggaaaggat tcaacctcga
     4141 tatcacacca gatgataacg cgattcttgc cagaggttgt gtcgacttta tcggctttag
     4201 ctactacatg tcttttacga cgcaattttc gccagataat ccgcaactgg attatgttga
     4261 accacgagat ttggtcagca acccttatat cgatacatcc gaatggggat ggcaaattga
     4321 tccggcaggg ctacgttatt cactcaactg gttctgggat catttccagt tgccgctgtt
     4381 tattgtcgaa aatggatttg gtgcggttga ccagagacaa gctgacggca cggtgaacga
     4441 tcactatcgc attgattact tttcctccca tattcgggaa atgaaaaaag ccgtagttga
     4501 agatggtgtt gacttaattg gctacacccc gtggggttgc attgacctgg tttctgccgg
     4561 aacaggagaa atgaaaaaac gctacggaat gatttatgtc gataaagaca acgaagggaa
     4621 gggaacgctg gaacggatac gtaaagcatc gttttactgg tatcgggatc tcatcgccaa
     4681 caatggcgaa aatatatgag acttcaggaa gatattacgg aaatgtcagg ctctgtgatc
     4741 agagcctgac aataactaca tagcgtgttg attataatag ggcacgccga gcgcatccga
     4801 cttatcactg ccagtaccca tgtgatttaa atcaaacggt ggttgttcct gcgatgtggg
     4861 aacaaccacg acattgccac aggaagtaac aaccggctgc gtggtttgtt ccgcaacgcc
     4921 ctgcgcagaa aagagaataa gcgcagttgc tattgcggat aaaagtggtt tcatgattgc
     4981 ctcagtgacg ttgtcacgtt atcaactagt tgttgtaatg atttaatgga tgtaaaaagc
     5041 gcgattcacc ctgcatattg gtcatccggt atttatgcgg cggaacatca aagtaatttt
     5101 tgaaggttcg ggtcagagtt tgttgggact cgaagccata tcgttccgcc agatagagta
     5161 tcggctcgtt actttccttc agcttttgcg cgatttccgt catcttacgg ctacggatgt
     5221 attggcctaa tgaatgaccg gtttcttttt taaacatccg ttgcaggtgc catttggagt
     5281 aacccgaacg ctctgacact ttctccagtg acagtggcga ttccaggttg tcctcgatcc
     5341 agtccaaaat gctatgaatg gtaatagcgt cagtattgcg tctggacatc gtcatacctc
     5401 ttttttcgtt tacggcagga ctttcttaag caaatgctca agtgttgcca cttcgtccgc
     5461 cgtcaggttt tttgttaatt cttgatgcag gtcctggcca actaattgat ggcattgttc
     5521 acatattgcc gcgccgctgg tggtaagttt taccagtacg ccgcgcttat cattcgggtt
     5581 cggcaacctt tccacccagc ctttacagac caggcgatcc agcatacggg tcagtgcccc
     5641 caggtcgacc gacaacactt ttttcagttc aaccggagta atacacgccg cgcagcggat
     5701 agagcagagc accttaaact gtgccgcggt aatatccagc ggagacagat actcgttaag
     5761 caggcgatct ttcttctgat taaccatatg gattaagcga cccaatggaa taatttcatt
     5821 gaacagatcg ctggtatttt tcacattagt tgccctggca agtaattagt tgcaggggat
     5881 aatattgccc aggcaagtat aagtcaaatg aatgaattga ccgatgccac gttttgctaa
     5941 atcggttcaa gttttggtca atgcgatgca aggttagcta aagagtgaca ctttatcctt
     6001 gtttacccct tatacttttc gctgataaac cagatacaca ggataacaac caccaatgtt
     6061 agatttgttt aaagcaatag gcctggggct ggtggtgtta ctgccgttag ctaacccatt
     6121 gacaactgtc gcgttatttc ttggcctggc aggcaacatg agcagtgccg aacgtaatcg
     6181 tcagtcgttg atggcctcgg tgtacgtatt tgccatcatg atggtggcgt attacgccgg
     6241 gcaactggtg atggatacat ttggcatttc gattcccggt ctgcgaattg caggcggctt
     6301 aatcgtcgcg tttatcggtt ttcggatgct ctttccgcaa cagaaagcga ttgattcacc
     6361 ggaagcgaaa agcaagtcgg aagagctgga agatgaaccc agtgccaata ttgcttttgt
     6421 accactggca atgccaagta ccgccggtcc gggaaccatt gcgatgatta ttagctcggc
     6481 ctccacagtg cgtcagagtt caaccttcgc tgattgggta ctgatggttg cgccgccgct
     6541 gatctttttc ctggtggcgg ttattttgtg gggaagtcta cgcagctctg gcgcaattat
     6601 gcggctggtg ggcaaggggg gtattgaagc gatctcccgt ctgatgggat tcctgctggt
     6661 atgtatgggc gtgcagttta ttattaacgg catcctggaa atcattaaaa cgtatcatta
     6721 aaaaaggccc gaatgggcct ttcgactatt gcgtctgttc ttcgagtgtc actgaccagc
     6781 ggcgaaaaat gatgattgac catattaacg cagcaaaagc aggcaccgcg cctacataac
     6841 caatcatcga cattgaccag tgcagactca cctgattacc taccaacgca cccgcgccga
     6901 ttccaatatt aaatatcccg gagaatagcg ccatcgcgac gtcggtggca tctggtgcca
     6961 gcgccagcac tttaacctgc ataccaagcc cgatgatcat catcgcgatc ccccagaaaa
     7021 tactcagcat cccgaggtgt atttcactgt tcgccgcagg tagcagcagt gccaggcaca
     7081 ccagcaatag cgcaatcgct gtactcacca acgcagacgc atactgatta cccagtttac
     7141 cgaaaatcac gctgccaata atgcccgcac caccgagtaa taacagtaat gccgtggcaa
     7201 agttggcgct gaatcccgca atgttttgta caaaaggctc gatatagctg tatgccgtgt
     7261 aatgggcggt gacaaccacc acagttaaca aataaatgct catcaatgcc gggcggcgga
     7321 acaatagcgg gaggcttttc aatgaaccgg aatgctcact gggcagcaag ggaagtaact
     7381 taatcaggca caaaagggta ataagcgccc cgataccaat cgcgaagaag gtcattcgcc
     7441 agccaaaata ctgccccaca atgcgcccga gaggtaaacc taacaccatt gccagtgctg
     7501 taccggtggc aattaaactc aatgcctgtg ctcgcttccc ggccggagcc atacggatcg
     7561 ccagagacgc cgtaatcgac cagaaaatcg catgtgcaaa agccacacca atgcgactga
     7621 tcaccagaac ggtaaaactc cacgataaaa acgacagtac gtggctggca ataaacacca
     7681 caaacaggca gatcagtaat ttgcggcgtt caacctggct ggtcattaac ataaaaggca
     7741 atgacattag cgccactacc catgcataaa ttgtcaacat gatgccgacc tgagcggttt
     7801 gcatgtgaaa actatgcgca atgtcagaga gcaggccaac agggacaaat tcggtggtat
     7861 tgaagataaa ggcggcgact gccagcgtaa cgacccgtag ccacgctact ttgcgggaaa
     7921 cagtgtttgt tgtcataaca gattatcggg tgagttgcga aaaagtgatg aaatgatctt
     7981 aaaacggtta atgatgtgaa tacaatgcat ttgtgatctg gatcgcattt ccatctctct
     8041 gacggttgtt ctgtcacgca tcagagagtt gttaacatca cattttaatg acgatcagga
     8101 agggggcctg ttcttctttt acttgaggtg caatattacg aacgggaaaa tcatcaggaa
     8161 gagatatgta attgctgatg cttactggcg taaccatatt tgccccatat agtcataagt
     8221 catcggaaca tcactatata gagcatatta gccgcatgaa ctatcaaaaa tagctcattg
     8281 gtaaccctgt tttgccgagt caggcgcatc cagcagttga ataaatgcct caagcggacg
     8341 cgttttcgca ccacgacgcc agaccagcca ggttgttaac caacgccatt gctcagctaa
     8401 cggccacgct tcaacctggt gatgccccgg catactttcc agcattgagc gtggaataag
     8461 cgcaatgcct gctcctgcga ccacacaggc caacatcccg tgataagact ccatctcgtg
     8521 aatagttccc ggagcggcac cgtcagcatg aaaccagctt tcgaagtggc gacgatacga
     8581 acaattggcg cggaaggcat aaatgttact gccattcacc tgactggcac gggttactgg
     8641 cgcatgtcct tgtggcgtga cgatcattag ttcctcgcgg tataccggta tcccgtcgat
     8701 ggcagtatgg ttaatgggtc catcaataaa cgccgcattc agttttccct ccagcacacc
     8761 ttccagcata gtgccggaag ggccggtgga aagtgaaaac tgaattttgg gataacgacg
     8821 gttaaattcc gccagcgtgg ccggaatgcg cactgcagcg gtgctttcca gcgatcccag
     8881 agaaaataaa ccttgcggct catcgccagc gacaacgctc cgcgcttcat ccactaacgt
     8941 gagaatttgt tggctatagc gtaaaaagtt atgcccggca ggcgagagac gtaaacgctg
     9001 attctcgcga ataaacagat caacccccag ttctgtttcc agctggcgta gacgggtagt
     9061 gagattcgac ggcacgcgat gcacttttgc tgcagcctgg gtaatgctgc cagcttcggc
     9121 aaccgcgttg aacatctcca gttgggtcag atccatagca ttctcgattc gtgaataagt
     9181 ggcttaatat tattcatttt aaagcaagag taaatctgcg tatcttcata ccatgactca
     9241 taaaggagat accccgatga ccattactcc ggcaactcat gcaatttcga taaatcctgc
     9301 caccggtgaa caactttctg tgctgccgtg ggctggtgtt gacgatatcg aaaacgcgct
     9361 tcagctggca gcagcaggct ttcgcgactg gcgcgaggca aatatagatt atcgtgctga
     9421 aaaactgcgt gatatcggta aggctctgcg cgcccgtagc gaagaaatgg cgcaaatgat
     9481 cacccgtgaa atgggcaaac caatcaacca ggcgcgcgct gaagtggcga aatcggcgaa
     9541 tttgtgtgac tggtatgcag aacatggtcc agcaatgctg aaggcagaac ctacgctggt
     9601 ggaaaatcag caggcggtta ttgagtatcg accgttgggg acgattctgg cgattatgcc
     9661 gtggaatttt ccgttatggc aggtgatgcg tggcgctgtt cccatcattc ttgctggtaa
     9721 cggctactta cttaaacatg cgccgaatgt gatgggctgt gcacagctca ttgcccaggt
     9781 gtttaaagat gcgggtatcc cacaaggcgt atatggctgg ctgaatgccg acaacgacgg
     9841 tgtcagtctg atgattaaag actcgcgcat tgctgctgtc acagtgaccg gaagtgttcg
     9901 tgctggagcg gctattggcg cacaggctgg agcggcactg aaaaaatgcg tactggaact
     9961 gggtggttcg gatccgttta ttgtgcttaa cgatgccgat ctggaactgg cggtgaaagc
    10021 ggcggtagcc ggacgttatc agaataccgg acaggtttgt gcagcggcaa aacgctttat
    10081 catcgaagag ggaattgctt cggcatttac cgaacgtttt gtggcagctg cggcagcctt
    10141 gaaaatgggc gatccccgtg atgaagagaa cgctctcgga ccaatggctc gttttgattt
    10201 acgtgatgag ctgcatcatc aggtggaaaa aacactggcg cagggtgcgc gtttgttact
    10261 gggaggggaa aagatggctg gagcaggtaa ctactatccg ccaacggttc tggcgaatgt
    10321 taccccagaa atgaccgcgt ttcgggaaga aatgtttggc cccgttgcgg caatcaccat
    10381 tgcgaaagat gcagaacatg cgctggaact ggctaatgat agtgagttcg gcctttcagc
    10441 gaccattttc accaccgacg aaacacaggc cagacagatg gcggcacgtc tggaatgcgg
    10501 tggggtattt atcaatggtt attgtgccag cgatgcgcga gtcgcctttg gtggcgtgaa
    10561 gaagagtggc tttggtcgtg agctttccca tttcggctta cacgaattct gtaatattca
    10621 gacggtatgg aaagaccggg tctgacgcta tatattcagc tctgatatac tcgcaggtct
    10681 tttcagacct gcggtccagg agtagaaagt ggcagtcgcc atggataatg caattttaga
    10741 aaacatcttg cggcaagtgc ggccgctcat tggtcagggt aaagtcgcgg attatattcc
    10801 ggcgctggct acggtagacg gttcccgatt ggggattgct atctgtaccg ttgacggaca
    10861 gctttttcag gccggagacg cgcaagaacg cttttccatt cagtctattt ccaaagtgct
    10921 gagtctcgtt gtcgccatgc gtcattactc cgaagaggaa atctggcaac gcgtcggcaa
    10981 agatccgtct ggatcaccgt tcaattcttt agtgcaactg gaaatggagc agggtattcc
    11041 gcgtaatccg tttattaatg ccggtgcgct ggtggtctgc gatatgttgc aagggcgatt
    11101 aagcgctcct cggcaacgta tgctggaagt cgtgcgcggc ttaagtggtg tgtctgatat
    11161 ttcctacgat acggtggtag cgcgttccga atttgaacat tccgcgcgaa atgcggctat
    11221 cgcctggctg atgaagtcgt ttggcaattt ccatcatgac gtgacaaccg ttctgcaaaa
    11281 ctactttcat tactgcgctc tgaaaatgag ctgtgtagag ctggcccgga cgtttgtctt
    11341 tctggcgaat caggggaaag ctattcatat tgatgaacct gtggtgacgc caatgcaggc
    11401 gcggcaaatt aacgcgctga tggcgaccag cggtatgtac cagaacgcgg gggagtttgc
    11461 ctggcgggtg ggactaccgg caaaatctgg cgttggtggc ggtattgtgg cgattgttcc
    11521 gcatgaaatg gccatcgcag tctggagtcc ggaactggat gatgccggta actcgcttgc
    11581 gggtattgcc gtccttgaac aattgacgaa acaattaggg cgttcagttt attaatgcag
    11641 tctctcgatc cactcttcgc gcgtttatcc cgttcaaaat ttcgctctcg ctttcgtttg
    11701 ggggtgaaag agcgtcagta ttgtctggag aaaggcgcgc cagtcattga acaacatgcg
    11761 gcggattttg tcgctaaacg tcttgctccg gcattaccgg ctaacgacgg taagcaaacc
    11821 cccatgcgcg ggcatccggt ttttatcgct caacacgcca ccgcgacctg ttgtcgcggt
    11881 tgtctggcta agtggcataa cattcctcag ggggaagcgt taagtgagga gcaacaacgt
    11941 tatattgtga cggtaatcta ccactggctg gttatacaga tgaatcagcc atgataatta
    12001 cggaaatcga taaaatggca ttcattaggt atctttagtt gccgccgttg tcagcgaaca
    12061 tgctatcctt tctaagaatt tacttattgc gttgagataa ttgccacccg caattgtatg
    12121 gacagataat taatgcatgt acagcccatt tcaactttcc gtttgttcca ggaaggtcat
    12181 ctgctacgta atagcatcgc tatttttgtg ctaaccacgc tgttctattt tattggtgca
    12241 gagttacggc tggttcacga actttctctt ttctggccgc tgaatggcgt aatggcgggg
    12301 gtgtttgccc gctatgtctg gcttaatcga ctgcattact atgcgatcag ttatgtggcg
    12361 atgctggttt atgatgccat aaccaccgaa tgggggctgg tttcactggc tatcaatttc
    12421 tccaatatga tgtttattgt taccgtcgcc ttactggtcg cgcgggataa gcgtctggga
    12481 aaaaataagt atgagccagt aagtgcctta cggctattta attactgtct gcttgccgca
    12541 ttattatgcg ctattgtcgg ggcgattggt tcggtcagta ttgatagtct ggatttctgg
    12601 cctttgcttg ccgactggtt tagtgagcaa ttctcaacgg gcgtgttgat cgtgccttgt
    12661 atgctgacgt tggcaattcc tggagtgctg ccgcgcttta aagcagagca gatgatgcct
    12721 gctatcgcgc ttattgtgtc ggttattgcc tcggtagtca ttggcggagc ggggagtctg
    12781 gcgtttccgc tccctgcatt aatctggtgt gcagtgcgct atacgccgca ggtaacatgt
    12841 ctgttgacct ttgtcaccgg tgcggtggaa gtcgtactgg tggcaaattc ggtgattgat
    12901 atctcggtcg gttcgccgtt ctccattccg caaatgttct ccgcacgtct cggtattgcc
    12961 acgatggcga tatgcccaat tatggtttcc tttagcgtgg cagcgatcaa ttcgctaatg
    13021 aagcaagttg cgctgcgagc cgactttgat tttctgactc aggtttactc acggtccggt
    13081 ctttatgagg cgctgaaaag tccatcgctg aaacagacac aacatctgac ggtcatgctg
    13141 cttgatatcg actatttcaa aagcattaac gataactatg gacatgaatg tggcgataaa
    13201 gtgttaagcg tgtttgccca gcatattcag aagattgtcg gtgataaggg gctggtggcg
    13261 cgaatgggcg gcgaagaatt tgctgttgca gtgccgtcgg tgaatcctgt cgatggcctg
    13321 ctaatggcgg aaaaaatccg taaaggcgta gaacttcaac cgttcacctg gcaacaaaaa
    13381 acgctttatc tcacggtaag tattggcgtc ggtagtggct gcgcatcgta ccgaacgctg
    13441 accgatgact ttaataaatt gatggtcgaa gccgatacgt gtctgtatcg ctcgaagaaa
    13501 gatgggcgca accgtaccag cactatgcgt tacggtgagg aagttgtctg acatctttat
    13561 ttactcagag cgaattaaat gcaatgtgtt ctaccacttt atgatccaag aaaaggcctg
    13621 tttcctcgat taaaccatga tccgcgccac acttttctca tacaactgct cattaatcat
    13681 caggagttga tcaaaacagg ggttcggcgt attaaagttt gctaacctgt cgtaccaatt
    13741 aaaacaggta gtatgactaa tcagaaggga acccattgtg aaaacactaa atcgtcgcga
    13801 ttttcccggt gcacagtatc cagaacgtat cattcagttt ggtgaaggta acttcctgcg
    13861 cgcctttgtt gactggcaaa tcgatctcct gaatgagcac accgatctga attctggcgt
    13921 ggtcgttgtt cgtccgattg aaacttcatt cccgccgtca ctgagcacgc aggatggtct
    13981 gtataccacc attatccgtg gcctgaatga gaagggtgaa gcggtcagcg acgcgcgtct
    14041 gattcgctcg gttaatcgtg aaatcagcgt ctacagtgaa tacgatgaat tcctgaaact
    14101 ggctcacaac ccggaaatgc gctttgtttt ctctaacacc accgaagcgg gtatcagcta
    14161 ccatgcgggc gataaattcg atgacgcgcc agcggtaagt tatccggcaa aactgacccg
    14221 tctgctgttc gaacgtttta gccatttcaa cggtgcgctg gataaaggtt ggatcatcat
    14281 cccgtgtgag ttgattgact ataatggcga cgcgttgcgt gagctggtgc tgcgctatgc
    14341 gcaagagtgg gcactgccag aagcatttat tcaatggctg gatcaggcta acagcttctg
    14401 ttctacgctg gtggaccgta tcgttaccgg ttatccgcgt gatgaagtgg ctaaactgga
    14461 agaagaactg ggctaccacg atggtttcct cgacaccgct gaacactttt acctgtttgt
    14521 gattcagggg ccgaaatcct tagcgactga acttcgtctg gacaaatatc cgctcaacgt
    14581 gctgatcgtt gacgatatta aaccgtataa agaacgcaaa gtggcaatcc tcaacggtgc
    14641 gcacaccgcg ctggtgccgg tggcgttcca ggcagggctg gataccgtag gtgaagcgat
    14701 gaacgatgct gaaatttgcg cattcgttga aaaagccatc tacgaagaaa ttattccggt
    14761 actggatttg ccacgcgatg agctggagtc tttcgccagt gcagtcaccg gacgtttccg
    14821 taacccgtac atcaagcatc aactgctgtc tatcgcgctc aacggtatga ccaagttccg
    14881 cacacgcatc ctgccgcagc tgctggcagg gcagaaggca aacggcacac ttccggcgcg
    14941 ccttactttc gcattagcgg cattgattgc gttctatcgc ggtgagcgta atggagaaac
    15001 atatccggta caggacgatg cacactggct ggaacgttat cagcaactct ggagccagta
    15061 tcgtgaccgt gttatcggga cccaggaact ggtagcgatt gtgttggcag aaaaagacca
    15121 ctgggagcag gacctgacgc aagtgcctgg tctggtcgag caggttgcta atgacctgga
    15181 tgcgattctc gaaaaaggga tgcgcgaagc tgtacgcccg ttgtgctaat actctgaacc
    15241 cggtttcgac cgggtttctt atttcactat attagttaca acatcctatg cgtctttatc
    15301 attaacaggt gccacatgca gcacctgtta atgttgtaag cttgtaggta atttgtaagt
    15361 agtgtcttga gaatgtgatc gtaatcacgt ttaatagggc gacttttcca cgcctgaaga
    15421 taaccatgat tgttcgtcca caacaacact ggctgcgccg tatttttgtc tggcacggtt
    15481 cagtattatc caagatatcc tcgcgcttac tactcaattt tctcttttct atcgctgtta
    15541 ttttcatgct gccctggtac acgcatttgg gcattaaatt caccctcgca ccgttcagca
    15601 ttctcggtgt cgccatcgcc atttttcttg gttttcgtaa taatgccggt tacgcccgtt
    15661 acgttgaagc gcgaaaactt tgggggcagt tgatgattgc ctcgcggtcg ttactgcgcg
    15721 aggtaaaaac gacattgccg gattcggcaa gtgtaaggga gtttgcccgg ctgcaaatcg
    15781 ccttcgccca ctgtttacgc atgacattac gcaaacagcc acaggcggaa gtgctggctc
    15841 attatctcaa gactgaagat cttcagcgtg tactggcttc taactctcca gctaaccaca
    15901 tcttgttaat aatgggggag tggttggcgg ttcagcgacg caatggacaa ctttcagata
    15961 tcctgtttat tagcctcaac gatcggctta atgatatttc agcggtcctg gcaggatgcg
    16021 agcgcattgc ctatacgcca attccctttg cctacaccct gattttgcat cgcaccgtgt
    16081 atctgttttg tatcatgctg ccgtttgcgc tggtcgtgga cctgcattac atgacgcctt
    16141 ttatctctgt gctgatttcc tacactttta tttcgctgga ttgtctggcg gaagaactgg
    16201 aagatccctt cggtactgaa aacaatgatt taccgctgga tgccatctgc aacgctattg
    16261 aaattgacct gttacagatg aacgatgaag cagaaattcc agcgaaaatt cttcccgatc
    16321 gccattacca gctgacgtga taattactcc gtacggcggg caacaataaa cagacgcgga
    16381 aatgccagca atatctgtcc gttctcctgg aggggatatt gctcttccag catctgatgg
    16441 tagcgcgtaa gaaaatgctg ctgttcgctc tcggtcagat cctgtaacca cggacgtaat
    16501 ccagtggcgg tcacccaatc gataatcgcc tggtgcgacg gcatctggtg atagtaagtg
    16561 gttcgccaga tatcgacctc acatccggct tcactcagaa tatcgtagta agcatgaacg
    16621 ccagccagcg gctcacgccc gcgatccggg tagttttgtt cccaggcaac ttcgcgcatg
    16681 agcacatggg tcggctccag ccagttatct ggcatctgta ctgccagcac gcccagcgga
    16741 ctgagtaacg aaaccaaatg aggaaacaat tcgtagtggt cgggcagcca ttgcagcgag
    16801 gcattagcaa aaatcagatc gagtgcctgt tccggttgcc agttgcggat atctgcttcc
    16861 acaaacaggc agtctggcaa agcactgcgc gcttcagcaa tcatcgccgg agatgagtcg
    16921 atgcccgtta tcctggcagc aggccaacgt tggtctagaa gggcggtgct gttacctggg
    16981 ccacagccaa gatcggcaat gtattcgata ttctccagcg gcaccctggc aagcagctcc
    17041 accgccggac gcgatcgttc agcggcaaag tgcagatata aagaggggtt ccagtcagac
    17101 atttttttac tcccgtcagc gcgttacccc aattgtagat aaaactgata aattcgcctc
    17161 acggcatcac cccattgaac agacgttttt tacgcggccc ggtcattaag attacagatt
    17221 ccggtagtca aagaagccac cgcattagga tgtgcttttt taaatcctcc tttttatccg
    17281 cgatcgcgga tatcgcagcg tttatcccgt agagcggata agatgtgttt ccaggttgac
    17341 tttatcctca ctaaaggata aaactgataa tatcccctta agaggataaa tttgctgtgg
    17401 acgtatgaca tgatgagctt tcagaagatc tatagcccaa cgcaattggc gaatgcaatg
    17461 aaactggttc gccagcaaaa cggctggacg cagagcgaat tggcgaaaaa aatcggcatt
    17521 aagcaggcga caatttccaa tttcgaaaac aatcctgaca atacctcgct cacgacattt
    17581 tttaagattt tacagtcgct tgaactctca atgacgctat gcgacgcgaa aaatgcctcg
    17641 ccagaagcag cagaacagca agatctggaa tggtaatgcc taaactagtc acttggatga
    17701 acaaccagcg ggtaggcgag ttaacgaagt tagccaacgg cgcgcacacc tttaagtatg
    17761 cacctgagtg gttagccagc cgttatgcca gaccgttgtc actttcgctg ccattgcaga
    17821 gggggaatat cacctctgat gccgtattta acttcttcga taacctgtta cccgatagcc
    17881 cgattgtacg tgaccggatc gttaaacgtt atcatgctaa atccagacaa catcatgcta
    17941 aatccagaca accgtttgat ttattgtcag aaatagggcg agatagcgtt ggtgccgtga
    18001 cgttaatccc cgaagatgaa accgtaacgt gtccgataat ggcatgggaa aagcttactg
    18061 aagccagact tgaagaagta ttaacggctt ataaagcaga tatcccgtta ggcatgatta
    18121 gagaagaaaa tgactttcgc atctcggttg ctggcgcaca ggagaagact gcgctgctca
    18181 gaataggcaa tgactggtgc tatggctttt ttaatggggt ccagcgaggc gctgaaagat
    18241 cgctatgatt ttatgaagtt tcaggtcttc cagtggttga ttggcgcaac agatggccat
    18301 gcaaaaaact tctccgtatt tattcaggct ggcggcagtt atcggctcac accattttat
    18361 gacatcattt cggcatttcc ggtccttggc ggtacgggaa tacacatcag cgatctcaaa
    18421 ctggcaatgg ggcttaacgc atccaaaggc aaaaaaacgg caatcgataa aatttatccg
    18481 cgacattttc tggcgacagc aaaggtgctg aaattcccgg aagtgcagat gcatgaaatc
    18541 ttgagtgact ttgccagaat gattccggca gcactggata acgtgaagac ttcattaccg
    18601 acagattttc cggagaactt ggtgacggca gttgaaacca atgtgttgag gttgcacgat
    18661 cggttaagcc gagaatacgg tattaagtaa gatatggggt ttttgtcgtt gttaatgagc
    18721 aggacaatca tgaaccgcca acgactggat tcgcccttgg tttatattgg tgcgcataat
    18781 gtgacatgcg ttatgttgaa aaggccgctg cgatactttc caccgcagcg gcctttttat
    18841 acataacgcc attgtgcgca ctgttgtgca ttcagtcgtt gcttctgttg attttattgt
    18901 gtgccctcag gacgattggc aacactattg ttatgacgct gaacaaaaac atgccgaaca
    18961 gactaccggc aatcaggtat ttcattagta aatccagaat catgtcgtgc atggtttttc
    19021 tgagctgatg ttgttaatcc tgtcgggact ctacattcct ttagctattt tttaaatcgt
    19081 gcttcctctt ccgtgtggcc gtaacgtttt gtttttacat tgtttattgt gcgatccaga
    19141 ttcagcaagt ctataaattt ttctagaaaa acaatatcat ctttctctat gtcccggatc
    19201 gcggccatta ggcgtatttt cgccgccgct tcacctgttt catgaccttg taacttaaaa
    19261 tctataccgt aaccaaaggc agtcatatct tcaccaaggt tctcaggcag aggcagggtt
    19321 gactcaccga atgctaacca taccggattt tttcctgtcg ctttagctat cttcctgatt
    19381 tctattaatt ttggttccct ttctccagaa gccagccgtt taagggtact gacgctgata
    19441 ccagtttcgt tttcgatatc gtcatatgta aaattttgga tgatagacag acgtatacgt
    19501 tctgcacact ctttgtctac tgccatatct gctgccttct catgctgatt gcaaagtctt
    19561 aattttaact tccatttttc atagctgaat ccaaggggtc agttttgata tttattggta
    19621 tcaacattat tgacgtttgt taagtttgat aatagcataa ctgataccgt caagtatcag
    19681 tactgagcct tgcggttcat cttgaatgat tgagagattc tatattttat gaccgaatca
    19741 gccatcctta cagcgtataa cgccatcgac gcggcatccg ttgcccttgg tcgtccctgc
    19801 ctgacgctgc ccgaacgcga gcattttcgg actgagcgcg atgttgccac ccgcaacctt
    19861 gctacgcttt cccgccagta tcgtgatgag gtcgggacct tatctgcaca gctcgcattg
    19921 ccgcgtaaca cgcaggttga ccgtgtgatt tacggaatta actgcacgga tttatctctc
    19981 gctgacaggg gcagggctgc cggactggta tcacagtttg gctcgttttc gttcaccgtg
    20041 gcgcatgctt tctgccgtct ggtttaccag ctcggtgtga aaaattcgct gatggcgctt
    20101 gagtccgcgc accgttttgc tttcttcaat caggacggag acccttatga attctcgctt
    20161 ttctgcactg atgagcagat
//
LOCUS       DAZFFE010000085        20089 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFFE010000085 DAZFFE010000000
VERSION     DAZFFE010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.085"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(421..1617)
                     /gene="hemY"
                     /locus_tag="ACHJ69_003382"
     CDS             complement(421..1617)
                     /gene="hemY"
                     /locus_tag="ACHJ69_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO5016302.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1620..2867)
                     /gene="hemX"
                     /locus_tag="ACHJ69_003383"
     CDS             complement(1620..2867)
                     /gene="hemX"
                     /locus_tag="ACHJ69_003383"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO5016303.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSTVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPAPAPQADAPAAAPQGE"
     gene            complement(2889..3629)
                     /gene="hemD"
                     /locus_tag="ACHJ69_003384"
     CDS             complement(2889..3629)
                     /gene="hemD"
                     /locus_tag="ACHJ69_003384"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO5016304.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDSLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSRKVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3626..4567)
                     /gene="hemC"
                     /locus_tag="ACHJ69_003385"
     CDS             complement(3626..4567)
                     /gene="hemC"
                     /locus_tag="ACHJ69_003385"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO5016305.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICKRE
                     DPRDAFVSNTYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4954..7500
                     /gene="cyaA"
                     /locus_tag="ACHJ69_003386"
     CDS             4954..7500
                     /gene="cyaA"
                     /locus_tag="ACHJ69_003386"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO5016306.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRREVLSQLVKEWEWDDSRLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQEHDAPLLQQYFS"
     gene            complement(7540..7860)
                     /gene="cyaY"
                     /locus_tag="ACHJ69_003387"
     CDS             complement(7540..7860)
                     /gene="cyaY"
                     /locus_tag="ACHJ69_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO5016307.1"
                     /translation="MNDSEFHRLADQLWLTIEEHLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7908..9224)
                     /locus_tag="ACHJ69_003388"
     CDS             complement(7908..9224)
                     /locus_tag="ACHJ69_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO5016308.1"
                     /translation="MNGQIITVALILLGNTFLTPPVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMNRADLRLVKNISGKTGYIPSDEISIENEEYHWVTDGHVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYLNGAH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQFHLITDNIAQYKDRLYVTKLSTFGEDQLEIIPLDTP
                     DLVIDDSFNSGKQHAYFIRQLRSRKSVQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVR
                     ITPSHCPAKTRVREENVREIHNSDIIIPVTDESCRNAAADGQTLKP"
     gene            complement(9221..10690)
                     /locus_tag="ACHJ69_003389"
     CDS             complement(9221..10690)
                     /locus_tag="ACHJ69_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO5016309.1"
                     /translation="MHYRNVVCLLSCSLFLSSAWGCRLDEPEHNIYQKQGKGVVYLRP
                     YEKTNLSLPQINYKRLRLLPNLLIDPTKLEDWETVPPATNLMTDVVYSGANATLPHYS
                     YYSDGRAILYAGEIVQNPPGTPPVDISSFQAWGDFAADKYSLYYEGKRTDSNQQLNRR
                     TLRQVEFNPQWKPDWLGLILRDKHYLYANGQRLDDPDTFTVLAQKSWDQRGKFSTAFN
                     PCLPAPFGPWDTLARTQTKIMINGEQLDADPDTFSVVRWMPGSLFTWRDKNGLQRKVL
                     DKENLAWDEDLTKHCLDFSLQEKKVFWRKGPACKQEELSGLDPEQFHPISDAVAQYQD
                     SLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAEGWVNHSSLHVFES
                     DGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYVYSFDGAQLWRYRTADPKQVRLI
                     WKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            10879..11082
                     /gene="yifL"
                     /locus_tag="ACHJ69_003390"
     CDS             10879..11082
                     /gene="yifL"
                     /locus_tag="ACHJ69_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5016310.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDSATGDGPSQVNY"
     gene            11119..11943
                     /gene="dapF"
                     /locus_tag="ACHJ69_003391"
     CDS             11119..11943
                     /gene="dapF"
                     /locus_tag="ACHJ69_003391"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO5016311.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            11940..12647
                     /gene="yigA"
                     /locus_tag="ACHJ69_003392"
     CDS             11940..12647
                     /gene="yigA"
                     /locus_tag="ACHJ69_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFO5016312.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLARWIERV"
     gene            12644..13540
                     /gene="xerC"
                     /locus_tag="ACHJ69_003393"
     CDS             12644..13540
                     /gene="xerC"
                     /locus_tag="ACHJ69_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO5016313.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIIHFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMSRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13540..14256
                     /gene="yigB"
                     /locus_tag="ACHJ69_003394"
     CDS             13540..14256
                     /gene="yigB"
                     /locus_tag="ACHJ69_003394"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO5016314.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRVDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14340..16502
                     /gene="uvrD"
                     /locus_tag="ACHJ69_003395"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14340..16502
                     /gene="uvrD"
                     /locus_tag="ACHJ69_003395"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO5016315.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(16547..17449)
                     /locus_tag="ACHJ69_003396"
     CDS             complement(16547..17449)
                     /locus_tag="ACHJ69_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFO5016316.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTERKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(17532..18296)
                     /gene="yigE"
                     /locus_tag="ACHJ69_003397"
     CDS             complement(17532..18296)
                     /gene="yigE"
                     /locus_tag="ACHJ69_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO5016317.1"
                     /translation="MAHRLFIGKGMITLNLKRIFLTLTLLPLFAVAADDCAPSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     LLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGEAIPWQRYPFVTMISVEQKG"
     gene            18458..18514
                     /gene="ysgD"
                     /locus_tag="ACHJ69_003398"
     CDS             18458..18514
                     /gene="ysgD"
                     /locus_tag="ACHJ69_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO5016318.1"
                     /translation="MDTPSRYWLIILSSRINS"
     gene            18666..19616
                     /gene="corA"
                     /locus_tag="ACHJ69_003399"
     CDS             18666..19616
                     /gene="corA"
                     /locus_tag="ACHJ69_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO5016319.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(19652..>20089)
                     /locus_tag="ACHJ69_003400"
     CDS             complement(19652..>20089)
                     /locus_tag="ACHJ69_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFO5016320.1"
                     /translation="LWTLRPVQILYTYSDAGNSVFLVVDHLPWTDSDKINWYLKHQDE
                     IKSQNPLPEDSWHTWYVIDIGNGFTDYKKYIEGPYEDLYCFPTIKSNDNCIVKNYLMV
                     INEYPYRNTHFFIADNNEYQLTQENKIERVFNPHDFEKDNFQQ"
BASE COUNT         4635 a         5162 c         5298 g         4994 t
ORIGIN      
        1 aattgaatga cttttattat tcattatata tatatgtgta gaattgtgcg caggagaaat
       61 attcactcag gaagttatta ctcaggaagc aaagaggatt acagaattat ctcataacaa
      121 gtgttaaggg atgttatttc ccggttctct gtggcataat aaacgagtag atgctcattc
      181 catctcttat gttcgcctta gtgcctcata aactccggaa tgacgcagag ccgtttacgg
      241 tgcttatcgt ccactgacag atgtcgctta tgcctcatca gacaccatgg acacaacgtt
      301 gagtgaagca cccacttgtt gtcatacaga cctgttttaa cgcctgctcc gtaataagag
      361 caggcgtttt tttatatatc agaaaggccc cggaggtgct tgcctccggg tgagaaagag
      421 ctactgtggc gggttgttct gcaacgttaa catcaaaccg tcgcgacgca tcgctgcggc
      481 ttcttccggc ttgtgcagtc tgtccagcgc gtcggcaagc catgcgtaat cgtaggcgtc
      541 cggacgttgt ttcagcgctg cgcggaaggc gagcgatgct tcctgccatt ctccgtgttt
      601 catcagcgac tgacccagtg tgctccacaa cagcgggcga tcgcccacgt ttttgatctg
      661 ttggcgcagc actttttcca gctgttccgg gttattggtt ttcagacgcg ggatcggcag
      721 cagcaggcga tcgtcatact ggcgtttcag gccgtcgatg ataatctgct gggcagtatc
      781 atgatcgtca cactcaataa gatgttccgc cattgccacc tgcaaggcca cctgatgacg
      841 cgttttccgg ctttggtttt tccaccagtt acgcaaacct tcactaccgt tatcggcacg
      901 cgcctgatcc atcaggccaa tccatgcctg ttgttccagc attgcacgat gttcttcatc
      961 accaacatgg gctttcgcca ttgatgggat gatatccagc agcgaactcc atgcaccagt
     1021 gcggatatac gcctgttccg ccagacgtaa tacttccgga tggcgtggcg taacttccag
     1081 cagcttatcc acgccgtgac gtgctgcatg gttttcatta cgggccagtt gcagacgtac
     1141 acgggtgatt tctaccggaa tggtgtcatt gccggccagc tccgctgcgc gttccagatg
     1201 ttggttggcg cgtgcttcat caccacgttg ttgcgcggct tcggctgcca gtagatagtt
     1261 caccaccggt tgttcagcgt gatcggcatt tttcgccatt agcttttcaa cttgctgata
     1321 atcgccttcc gccagtttca gcagcgcctg ttcggtctgc ttacgtgcac ggcgacgctt
     1381 acgtccgaca aaccacccac gggtgtgcgc gcctgtgcgg aagattcgcc gcagtagcca
     1441 ctcaatggca aacagcacta ccatcgccag aatcaaaata atcgcgaggc ccgtgacgct
     1501 agtttcaata ttgtagttgt cggtctggat cagcacgtaa ccctgatggc cggcaatcat
     1561 cgggccaacc acgatccccg caatcagcaa cacaaagagc aataacactt ttagcatggt
     1621 tattctcctt gcggcgcggc tgccggagca tcagcttgcg gtgcaggtgc aggtgcaggt
     1681 gcaggtgcag gtgcaggtgc aggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt
     1741 ttagcttccg ttgtccctgc tgccggttgt gccagcaggt tacgcacgcg agtctgcatc
     1801 agtttttcca gcatcgcctg gctttgcagg gtttccggga gatccatcga gatattttgc
     1861 tggcttaact ggtccacctc gtcgaggaac gctttggtgg tggcatcatc agtatcgtag
     1921 taagcacgta cccaggtgga gacattctcc agcgcctggc gataagtctc ttcctgatga
     1981 cgcggtacag cttgtgcagc gaccagcagg cgagagcgaa tattttcgcg cagatagata
     2041 tcctgatttg gcgctaacag cggtacggcg gtgtcatcac gacggcgaat cgtaatgaag
     2101 ttgtccataa agttctgcca gcttttttgc agattgatac gccattcgct gatggaactg
     2161 gaaagctctt caccgtcgga atccatcggc gaaccatcgc tgtcattatc agccagacgc
     2221 aggttatcta cctgatttga aagctgatta agcttaagga taatgccgtc ataatccacc
     2281 tgcgataccg cagaaaggct ggcgatatca tcggtaattg cccggcgaac ggtaatcaga
     2341 ctcgggtcat tcatatccgc caggctggcg tctgcacttt tcagcaacgc tgcagcggtc
     2401 gtgacgtcct gatcgctcca cagcttccgt ccggcgagtt tcaccagaaa atcggcctgg
     2461 gccagcagcc aggttttagc atcgctgccg gaaatggtgg cgaccttttg ttggacttca
     2521 tccagctgtt ttgccagcgt ttcttgctga cgattcgcct gctcaagctg tacagcttgt
     2581 tgcttgataa tgccttccag ctcggctttt tggctctcct gggctttttg cagtgccgta
     2641 agttggttag ccagggcatc gctggtggca gtctgattga cggcctgttg tttaccccag
     2701 ccatacaaac cgacgcccgc cgccagagca atagcgatgg ccaccgtgct gagaatcaat
     2761 gcggtattgt tcttactctt tttttctgtt gcgacaggtt gtgacgtggt gtccacggcc
     2821 tccctggtct cttcaaccac ggcggaggtt ttttcttgtt ccgtcattat ggcttcctgt
     2881 tatgagagtt attgtaatgc ccgtaaaagc gcatcgttgt cagcgttatc ggcgacctta
     2941 atgtcttgcc agcccagttc ccgggcgagt ttcgccaaac gctcactgac gactaatagc
     3001 cgacagcgta gtaaccagtg ctcacgatac cattgtggga tcagcgacca gagttgctgc
     3061 aacatttcac cgctggtaac aacgaccgtc gtcaccttgc gggattgcca gcgcatcgct
     3121 tcttctgcac catcgtaatg gattgcgcat cgttgataac attcacaaaa agtgacctca
     3181 gcaccgcgcg ccgtcaggga atccccaatt agctcacgac cgccattgcc acgtaatatc
     3241 agcgcacgtt tgcccgcaat attttgtaat tcaggtaatt gtagcaagac ttcgctgatt
     3301 tcccgatcct gcgggtagag aatcttctgt ccgcttacgg tatgtagtgc cagcgcggtg
     3361 gtgcgtccaa tggcgaaata atcaggtagt cggggccatt tacgatcttg ctgatgcagc
     3421 tgtgattggg caaaagcaac cgcgtgttgc gagagggcaa acaacagatc gctctctccc
     3481 agcgctgcca gttgatcagc aagttgcggt aattgtcgac ccggagaaaa ctcaatcagc
     3541 ggaaaatgcc aggccacctg ccccagtgtg cgcagacggc tcactaactc ttctcctgcg
     3601 ggagacgggc gggtgaccag gatactcatg ccggggcgtc tccgttatag acttcagcga
     3661 ggatctcgcg cgcgccgtta ttcagtagct cttctgccag cgaaatcccc atttgttcgg
     3721 catcttgcgg cgcaccgcgg cgttcaccgc gaataatctg cgaaccgtcc ggcgcgccga
     3781 ccaacgcacg cagccagatt tcgccatcaa taagctcggc gtagctacca attggcacct
     3841 gacatccgcc ttcgagacgg gtattcatgg cgcgttcggc ggtaacgcgc agtgcagttt
     3901 cgtggtgatt cagcgcggcg agcagctcgc gagtgcgtgc atcatcaagg cggcattcaa
     3961 tgcccaccgc accttgccct accgccggaa gagaaatctc gggtggcaac gcggcgcgaa
     4021 tgcgggactc cagacctaaa cgttttagtc cggctacggc aagaatgatg gcatcatatt
     4081 cgccgttatc cagtttgctc agacgagtgc cgacgttgcc gcgcagggag cggataatca
     4141 gatccgggcg gcgttcagcc agttggcact ggcgacgtaa actggacgtc ccgacgatac
     4201 tgcctgccgg taacgcatcc agactgtcat aggtgttgga tacaaaggca tcgcggggat
     4261 cttcgcgctt gcaaatggtg accagcccca gaccttgcgg gaattcaacc ggcacatctt
     4321 tcattgaatg tacggcgata tcggcgcgat tttcgaggag cgcgacttcc agctctttca
     4381 caaataagcc ttttccgcct actttcgcca gcggcgtatc aagaatcaca tcgccgcgcg
     4441 tcaccatcgg taccagttca acgaccaggc ccggatggct cgccatcaac ttgtctttga
     4501 catagtgtgc ctgccagagt gcaagtgggc tttggcgtgt ggcaattctt aaaacattgt
     4561 ctaacatgct tgttaccgtc attatcatcc gtggtccatc ctaacatcct tgccagagcg
     4621 atgtcagtgt tgtggtgaaa cgtagacgcc tgcgcaaacc gtaaaatgag gtctggcagt
     4681 ggatcctgac aggcgctttg agccttcgta ataaggaatt tacagagaat aaacggtgct
     4741 acacttgtat gtagcgcatc tttctttacg gtcaatcagc aaggtgttaa attgatcacg
     4801 ttttagacca ttttttcgtc gtgaaactaa aaaaaccagg cgcgaaaagt ggtaacggtt
     4861 acctttgaca tacgaaatat cccgaatgcc gcgtgttacc gttgatgttg gcggaatcac
     4921 agtcatgacg ggtagcaaat caggcgatac gtcttgtacc tctatattga gactctgaaa
     4981 cagagactgg atgccataaa tcaattgcgt gtggatcgcg cgcttgctgc tatggggcct
     5041 gcattccaac aggtctacag tctactgccg acattgttgc actatcacca tccgctaatg
     5101 ccgggttacc ttgatggtaa cgttcccaaa ggcatttgcc tttacacgcc tgatgaaact
     5161 caacgccact acctgaacga gcttgaactg tatcgtggaa tgtcagtaca ggacccaccg
     5221 aaaggtgagc ttccaattac tggtgtatac accatgggca gcacctcgtc cgtagggcaa
     5281 agttgttcct ctgacctgga tatctgggtc tgtcatcaat cctggctcga tagcgaagag
     5341 cgccagttgc tacaacgtaa atgtagtctg ctggaaagct gggccgcctc gctgggtgtg
     5401 gaagtcagct tcttcctgat tgatgaaaac cgcttccgtc ataatgaaag cggcagcctg
     5461 gggggcgaag attgtggctc cacccagcat atactgctgc ttgacgaatt ttatcgtacc
     5521 gccgtgcgtc tcgccggtaa gcgtattctg tggaatatgg tgccgtgcga cgaagaagag
     5581 cattacgacg actatgtgat gacgctttac gcgcagggcg tgctgacgcc aaatgaatgg
     5641 ctggatcttg gtggcttaag ctcgctttct gctgaagagt actttggtgc cagcctttgg
     5701 cagctctaca agagtatcga ttccccatac aaagcggtgc tgaaaacact gctgctggaa
     5761 gcctattcct gggaataccc gaacccacgt ctgctggcga aagatatcaa acagcgtttg
     5821 cacgacggcg aaatagtgtc gtttggtctc gacccgtatt gcatgatgct ggagcgcgtc
     5881 actgaatatc tgacggcgat cgaagatttt actcgtctgg atttagttcg ccgatgcttc
     5941 tatttaaaag tttgcgaaaa actcagccgt gaacgcgcct gcgtaggctg gcgtcgtgaa
     6001 gtgttaagcc agttggtgaa agagtgggaa tgggacgatt ctcgtctggc aatgcttgat
     6061 aaccgcgcta actggaagat agatcaggtg cgtgaggcgc acaacgagtt gctcgacgcg
     6121 atgatgcaga gctaccgcaa tctgatccgc tttgcgcgtc gcaataacct tagcgtttcc
     6181 gccagcccgc aggatatcgg tgttctgaca cgtaaactgt acgccgcgtt tgaagcgtta
     6241 ccgggtaaag tgacgctggt aaacccgcag atttcacccg atctctcgga accgaatctg
     6301 acctttattt atgtgccgcc gggccgggct aaccgttcag gttggtatct gtataaccgc
     6361 gcgccaaata ttgagtcgat catcagccat cagccgctgg aatataaccg ttacctgaat
     6421 aaactggtgg cgtgggcatg gtttaacggc ctgctgacct cgcgcacccg cttgtatatt
     6481 aaaggtaacg gcattgtcga tttgcctaag ttgcaggaga tggtcgccga cgtgtcgcac
     6541 catttcccgc tgcgcttacc tgcaccgaca ccgaaggcgc tctacagccc gtgtgagatc
     6601 cgccatctgg cgattatcgt taacctggaa tatgacccga cagctgcgtt ccgcaatcag
     6661 gtggtgcatt ttgatttccg taagctggat gtcttcagct ttggcgagaa tcaaaattgc
     6721 ctggtaggta gcgttgacct gctgtatcgc aactcgtgga acgaagtgcg tacgctgcac
     6781 ttcaacggcg agcaatcgat gatcgaagcc ctgaaaacta ttctcggcaa aatgcatcag
     6841 gacgccgcac cgccagatag cgtggaagtc ttctgttata gccagcatct gcgcggctta
     6901 atccgtactc gcgtgcagca actggtttct gagtgtattg aactgcgtct ttccagcacc
     6961 cgccaggaaa ccgggcgctt caaggcgctg cgcgtctccg gtcaaacctg gggcttgttc
     7021 ttcgaacggc tgaatgtctc ggtacagaaa ctggaaaacg ccatcgagtt ttatggcgct
     7081 atttcgcata acaaactgca cggcctgtca gtgcaggttg aaaccaatca cgtcaaatta
     7141 ccggcggtgg tggacggctt tgccagcgaa gggatcatcc agtttttctt cgaagaaacg
     7201 caagacgaga atggctttaa tatctacatt ctcgacgaaa gcaaccgggt tgaggtgtat
     7261 caccactgcg aaggcagcaa agaggaactg gtgcgtgacg tcagtcgctt ctactcgtct
     7321 tcgcacgacc gtttcaccta cggctcaagc ttcatcaact tcaacctgcc gcagttctat
     7381 cagattgtga aggttgatgg tcgtgaacag gtgattccgt ttcgcaccaa atctatcggt
     7441 aacatgccac ctgccaatca ggagcatgac gcgccgctgt tgcagcagta tttttcgtga
     7501 tgaacgtgcc ggaaagcgag gcttatccgg catgcaatct tagcggaaac taactgtttc
     7561 acccgcctgc tgcgtcgccg cctgttccag caaatcccag aaggtttcac cgctacgatc
     7621 gcaaatccac tcgtcgcctt tcagatcaaa atggtagcca ccctgtttgg ttgccagcca
     7681 cacctggtgc agtggttcct ggcggttgat aatgattttg ctgccgttct caaaggtaat
     7741 ggttagtaca ccgccgttga tttcacagtc gatatcgctg tcgccatccc agtcgtccag
     7801 atgttcttca atggtcagcc acagttgatc agccaggcga tgaaattcac tgtcgttcat
     7861 tgttgtatcc tgtttttaag tgatggcggc agtatagcgg catggggtca gggcttcaaa
     7921 gtttgcccgt ctgctgctgc gttccggcat gattcatccg tcaccggaat aataatgtcg
     7981 ctgttatgaa tttcacggac attctcttct ctgacgcgtg ttttcgccgg gcagtgggag
     8041 ggcgtaatcc ttacctctgt atcagtccag gtataaacat tgcgatcgtc ataagcgaag
     8101 cgatcgtttt tagttagcgg accgttaact ggaataattt gcacgctctt gcgtgaccgt
     8161 aattggcgga taaagtaagc atgttgtttg ccgctattaa atgagtcatc aatgaccagg
     8221 tctggcgtat ccagcgggat tatctcaagc tggtcttcac caaaagtcga taattttgtt
     8281 acatacagtc gatctttata ctgcgcaata ttgtcagtaa tcagatgaaa ttgttcaggt
     8341 tcaatacccg gtaggtctat ccacttgctc cactgacggt cgggcgtgag ctgtttacgc
     8401 cagcgaacgc gactttcccc tacttcaaaa gagacgtcat catctacggg tatcgctttg
     8461 ccagataatt gaccaaaggg ataacgatgc agacccttat tgtcgcgaaa aaccagcagt
     8521 gaatgaggta tccagcgaat tatctcaaat gagtctgcat tcgccgtaag gtgagcgcca
     8581 tttaaataaa tattctgccc actgcggata agcagatcat tggaatacaa atttctgttt
     8641 acactcataa gacgtggatt aataccccac catctttttt ctaatacagt aacattactg
     8701 ctgctcccct gacgtcgacc ggacaagtag agatttttac tatccaccag cgtggtagag
     8761 ttaccttcga tagcttttag tgtcgccaga tcaacacgtg acgcgccgct attactttcg
     8821 gtacgctgtc cgtcaaaata gaggctatat ttatcgaccg cgaagcggcc catagcctga
     8881 aagctggcaa tatcgacagt gggtgttccc ggtggattgc taacgatttt gccacgccag
     8941 agaataacgt ggccgtctgt cacccagtga tattcctcat tttctatgct tatttcatcg
     9001 gaaggtatat aacccgtctt cccagagata tttttcacaa ggcgcaaatc ggccctgttc
     9061 atctccggaa attgggcaat gattttctta tccccccaat aacctttacc gtaagacatg
     9121 acaccgtgat cattgtaata gtcgaaccct atggcctgta caggaggtgt cagaaatgtg
     9181 ttccccaaca ggatgagtgc aactgtgatt atttgaccat tcattttgtt gcctcattgc
     9241 gtggggcagg aataaattcg ccgtcatcgg taatttttcc gtccagatat cccgttttgt
     9301 aatttacgcc atagccatat cccgaatgtt gttccttcca gattaagcga acttgtttcg
     9361 gatcagcggt gcggtatcgc cagagctgcg cgccatcaaa gctgtaaacg tagcggttat
     9421 cgcgggcata ccatctgcgc aaaaagggat ggtcattaag atgggcttcg cgttcatccg
     9481 gagagcggtt atccagtaag atcagcggtc cgtcagattc aaacacgtgt aaactggaat
     9541 ggtttaccca cccttcggca cgcgtaagta aatagccgtg gcgtttcccg gcgttgaaac
     9601 gtttgttgat aataagattg gggtcatcga gttttacgat ctccaacttg cggtcaccag
     9661 actctgtttc gatgatggta taaagcgaat cctgatactg ggcgacagca tcactgatgg
     9721 ggtggaactg ttcaggatcg agtccggata attcttcctg tttacaagca ggccctttac
     9781 gccagaacac tttcttttcc tgcagagaaa aatccagaca gtgctttgtt aaatcttcat
     9841 cccacgccag attttccttg tcgaggactt tacgctgcag cccgttttta tcacgccagg
     9901 tgaagagtga gccgggcatc cagcgcacga cggaaaaggt gtccgggtcg gcatccagtt
     9961 gttctccgtt gatcatgatt ttcgtctgtg tgcgggctag cgtatcccag gggccaaatg
    10021 gggcaggaag gcagggatta aatgctgtag agaatttacc gcgctgatcc catgactttt
    10081 gagccagtac cgtaaaggtg tcgggatcat caaggcgctg accatttgcg taaagatagt
    10141 gcttgtcacg gagaatcaaa cctagccagt ctggtttcca ttgcgggtta aattctacct
    10201 gacgcaacgt tcggcggttt agttgctggt tgctatcggt gcgtttgcct tcgtaataga
    10261 ggctgtactt atctgcggca aaatcacccc atgcctgaaa tgacgagata tcgactggcg
    10321 gtgtgcctgg agggttttgc acaatctcgc cagcgtagag aatagcgcga ccatcgctat
    10381 agtaagagta atggggaaga gttgcgtttg cgccgctgta gacaacgtct gtcatgagat
    10441 tggtagccgg gggcaccgtt tcccaatcct ccagtttggt tgggtcaatt agcaaattcg
    10501 gtaacagacg cagacgcttg tagttaatct gcggtagcga aagattggtc ttttcataag
    10561 ggcgcaaata caccacgcct tttccctgtt tctggtaaat attatgttcc ggctcatcaa
    10621 gccgacatcc ccatgccgac gataaaaaca acgaacaaga caataaacaa accacattgc
    10681 gatagtgcat aaagccatcc tggcgcgagg tgccgatcac gaaactacca gcaaaacata
    10741 aatccccacg tgtaagcgtt atactcgcag catttcctca cttttcagac ttcataaaga
    10801 gtcgctaaac gcttgctttt acgccttctc ctgcgatgat agaaagcaga aagcgatgaa
    10861 ctttacaggc aatccataat gaaaaacgtg tttaaggcac tcactgtatt acttactctc
    10921 ttcagcctga cgggctgcgg tctgaaaggt ccgctctatt tcccgcctgc agataaaaac
    10981 gcaccgccgc cgaccaaacc ggtagagacg caaacgcaat ccacggtgcc ggataaaaac
    11041 gacagcgcca ctggcgatgg tccatcccag gtgaattact aaaagtcagt ttctgtaccc
    11101 gcgtgattgg agtaaatgat gcagttctcg aaaatgcatg gccttggcaa cgattttatg
    11161 gtcgtcgacg cggtaacgca gaatgtcttt ttttcaccgg agctgattcg tcgcctggcg
    11221 gatcggcacc tgggggtagg gtttgaccaa ttgctggtgg ttgagccgcc gtatgatcct
    11281 gaactggatt ttcactatcg cattttcaat gctgatggca gtgaagtggc gcagtgcggc
    11341 aacggcgcgc gctgctttgc ccgttttgtg cgcctgaaag ggctgaccaa taagcgcgat
    11401 atccgcgtca gcaccgccaa cgggcggatg gttctgaccg tcaccgatga tgatctggtc
    11461 cgcgtaaata tgggcgaacc caacttcgaa ccttccgccg tgccgtttcg cgctaacaaa
    11521 gcggaaaaga cctatattat gcgcgccgcc gagcagacaa tcttatgcgg cgtggtgtcg
    11581 atgggaaatc cgcattgcgt gattcaggtc gatgatgtcg ataccgcggc ggtagaaacg
    11641 cttggtcctg ttctggaaag ccacgagcgt tttccggagc gcgccaatat cggttttatg
    11701 caagtggtta agcgcgagca tattcgttta cgcgtttatg agcgtggggc aggagaaacc
    11761 caggcctgcg gcagcggcgc gtgtgcggca gtggcggtag ggattcagca aggtttgctg
    11821 gccgaagaag tacgcgtgga actccctggc ggtcgtcttg atatcgcctg gaaaggtccg
    11881 ggtcacccgt tatatatgac tggcccggcg gtacatgtct acgacggatt tattcatcta
    11941 tgaagcaacc aggggaagaa ctgcaggaaa cactcacgga gcttgatgac cgggcggttg
    12001 tcgattatct gattaaaaat cctgagtttt ttatccgcaa tgcgcgcgca gtagaagcga
    12061 tacgtgtgcc gcatccggta cgcggcaccg tttcgttggt cgagtggcac atggcccgcg
    12121 cacgtaatca tattcatgtt ctggaagaga acatggcgtt gttgatggaa caggctatcg
    12181 ccaacgaagg cctgttttat cgtctactct acctgcagcg cagtctcacc gccgccagca
    12241 gtctcgatga tatgctgatg cgctttcacc gctgggcgcg cgatctcggc ttggcaggtg
    12301 cgagtctgcg cctgtttcca gatcgctggc gcttaggtgc gccgtcgaac cacactcatc
    12361 tggcattaag ccgtcagtct ttcgaaccgc tgcgtattca gcgtctgggg caggaacagc
    12421 actatctggg accgcttaac ggaccggagc tgctggtggt gctaccggaa gcgaaagcgg
    12481 tgggatcggt ggcgatgtcg atgctgggaa gcgatgctga tttgggggtt gtgctattta
    12541 ccagtcgcga tgccagtcac tatcaacaag ggcagggaac gcagttactt catgaaattg
    12601 cgctgatgtt gccggagctt ctggcgcgtt ggattgaacg cgtatgaccg atttacacac
    12661 cgatgtagaa cgctacctac gttatctgag cgtggagcgc cagcttagcc cgataaccct
    12721 gcttaactac cagcgacagc ttgaggcgat catccatttt gccagcgaaa acggcctgca
    12781 aagctggcag caatgtgatg tgacgatggt gcgtaatttt gctgtacgca gtcgccgtaa
    12841 agggctggga gctgcaagtc tggcgttacg gctttctgcg ctacgtagct tttttgactg
    12901 gctggtcagc cagaatgaac tcaaagctaa cccggcgaaa ggtgtttcgg caccgaaagc
    12961 gccgcgtcat ctgccgaaaa acatcgacgt cgatgatatg agtcggctgc tggatattga
    13021 tatcaacgat cccctcgccg tgcgcgatcg ggcaatgctg gaagtgatgt acggcgcggg
    13081 tctgcgtctt tccgagctgg tggggctcga tatcaaacac ctcgacctgg agtctggcga
    13141 agtgtgggtg atgggaaaag gcagcaaaga gcgccgcctg ccgattggtc gcaacgccgt
    13201 ggcgtggatt gagcactggc ttgatttgcg cgacctgttt ggtagtgaag acgacgcgct
    13261 tttcctgtcg aaactgggca aacgcatctc cgcgcgtaat gtgcagaaac gctttgccga
    13321 atggggcata aaacaagggc tgaataatca cgttcacccg cataaattac gtcactcgtt
    13381 cgctacccat atgctggagt cgagcggcga tcttcgtggt gtgcaggagc tgctgggtca
    13441 tgccaacctc tccaccacgc aaatctatac tcatcttgat tttcaacacc ttgcctcggt
    13501 gtacgatgcg gcgcatccac gcgccaaacg ggggaaataa tgcgttttta ccggcctttg
    13561 gggcgcatct cggcgctcac ctttgacctg gatgataccc tttacgataa ccgtccggtg
    13621 attttgcgca ccgagcgaga ggcgcttacc tttgtgcaaa attatcatcc ggcgctgcgc
    13681 agcttccaga atgaagatct gcaacgcctg cgccaggcgg tacgggaagc ggaacctgag
    13741 atttatcacg acgtgacgcg ctggcgtttt cgttcgattg aacaagcgat gctcgacgcc
    13801 gggctgagtg ccgaagaagc cagtgcaggc gcacatgcag caatgatcaa ctttgccaaa
    13861 tggcgcagcc gggtcgacgt cccgcagcaa acccacgaca ccttaaaaca gctggcgaag
    13921 aaatggccgc tggtggcgat caccaacggt aacgcccagc cggaactgtt tggtttgggg
    13981 gattattttg agtttgtgct gcgcgctggc ccgcacgggc gctcaaaacc gttcagcgat
    14041 atgtacttcc tggcggcgga aaaactcaac gtgccgatcg gcgagatctt acatgtcggg
    14101 gacgatctca ccactgacgt gggtggggca attcgcagcg gaatgcaggc ctgttggatc
    14161 agaccggaaa atggcgatct gatgcaaacc tgggacagcc gtttactgcc gcatctggaa
    14221 atttcccggt tggcatctct gacctcgctg atataatcag caaatctgta tatataccca
    14281 gctttttggc ggagggcgtt gcgcttctcc gcccaaccta tttttacgcg gcggtgccaa
    14341 tggacgtttc ttacctgctc gacagcctta atgacaaaca gcgcgaagcg gtggccgcgc
    14401 cacgcagcaa ccttctggtg ctggcgggcg cgggcagtgg taagacgcgc gtactggtgc
    14461 atcgtatcgc ctggttgatg agcgtggaaa actgctcgcc gtattcgatt atggcggtga
    14521 cgtttaccaa caaagcggcg gcggagatgc gtcatcgtat cgggcaactg atgggcacta
    14581 gccagggcgg tatgtgggtc ggcaccttcc acgggttggc gcaccgcctg ctgcgtgcgc
    14641 accatatgga cgccaacctg ccgcaggatt tccagatcct cgacagtgaa gaccagctac
    14701 gtctgcttaa gcgtttgatc aaggcgatga atctcgacga gaagcagtgg ccgccgcgtc
    14761 aggcaatgtg gtacatcaac agccagaaag atgaggggct gcgtccgcat catattcaga
    14821 gctacggtaa tccggtggag cagacctggc agaaggtgta tcaggcgtat caggaagcgt
    14881 gcgaccgtgc gggcctagtg gatttcgccg agctgttgct gcgcgctcac gagttgtggc
    14941 ttaacaagcc gcatatcctg caacactacc gcgaacgttt taccaatatc ctggtggatg
    15001 aattccagga cactaacaac attcagtacg cgtggatccg cctgctggcg ggtgacaccg
    15061 gcaaagtgat gattgttggc gatgacgacc agtcaatcta cggctggcgc ggggcgcagg
    15121 tcgagaatat tcagcgtttc ctcaatgatt ttcccggtgc tgaaactatc cgtctggagc
    15181 agaactaccg ctctaccagc aatattctga gcgccgctaa cgccctgatt gaaaacaata
    15241 acgggcgtct gggtaaaaaa ctgtggaccg atggcgcgga cggtgagcct atttccctct
    15301 attgcgcttt taacgaactc gacgaagcgc gttttgtggt taaccgcatc aaaacctggc
    15361 aggacaacgg cggggcgctt gccgagtgcg ccattctcta ccgcagcaac gcccagtcgc
    15421 gtgtgctgga agaggcgtta ttgcaggcga gtatgccgta tcgtatttac ggcgggatgc
    15481 gcttcttcga acgccaggaa atcaaagatg cgctctcgta tctgcgcctg attgccaacc
    15541 gcaacgacga cgcggccttt gagcgtgtgg tgaatacgcc aacgcggggt attggtgacc
    15601 ggacgctgga cgtggtacgt cagacatcgc gcgatcgcca gttaacactc tggcaggcat
    15661 gtcgtgaact gttgcaggaa aaagccctcg ccggacgtgc tgccagcgcc ttacagcggt
    15721 ttatggagct gatcgacgcc ttagcgcagg aaactgccga tatgccgctg catgtacaga
    15781 ctgaccgggt aattaaagac tccggcctgc gcaccatgta cgagcaggag aagggcgaaa
    15841 aaggtcagac gcgtatcgaa aacttagagg aactggtgac ggcaacgcgc cagttcagct
    15901 acaacgaaga agacgaagat ttaatgccgc tgcaggcatt cctctcccat gcggcgctgg
    15961 aagcgggcga ggggcaggcg gatacctggc aggacgcggt gcagttgatg acgctacact
    16021 cggcgaaagg tctggaattc ccgcaggtgt ttatcgttgg tatggaagag ggcatgttcc
    16081 caagccagat gtcgctggat gaaggcgggc gtctggaaga agaacgccgt ctggcctacg
    16141 ttggcgtaac ccgcgcgatg cagaaactga cgctgaccta cgcggaaacc cgccgtctgt
    16201 atggcaaaga ggtttaccat cgcccgtcgc gctttatcgg tgagttgccg gaagagtgtg
    16261 tggaagaggt gcgcctgcgc gccacggtaa gccgcccggt cagccatcaa cggatgggga
    16321 cgccgatggt cgagaacgac agcggctaca agctcggcca gcgcgtacgc cacgctaagt
    16381 ttggtgaagg caccatcgtc aatatggaag gcagcggtga gcatagccgt ttgcaggtgg
    16441 cgttccaggg ccagggtatt aaatggctgg tggcggctta cgcccggttg gagacggtgt
    16501 gacgttgcgg aatgcggtgc gccataccgc attccgcatc agcaccttac ttagcgcact
    16561 tcttcacatc aatattccag tcagcaccag acgcaacctg atgtttctgc gcgaaattat
    16621 ccatattcag cgccacggaa acattcaata agctgttcag atacaccatc ggctggcctt
    16681 ccggcacatc gccaaagctt gcgacatacg gcattttccc tgcgtatttt tgctgggaac
    16741 cttcggaaat ggtcacacac aacgtgtcgt tgagtttgat tcctgcctga ttcagcaact
    16801 cgtcgctgat attgctccag acgttgccgt actgaatatc gagaatcggg atattgccct
    16861 tcacttcacc ttttgttgct ttggctttct gataagaaag ttcgacaact tttgcaggaa
    16921 gctctggccc cacctgctcg aacgtgatcg ccccggaagc cagacgtgca ccggtgtagg
    16981 catacacgtc acgaccgtgg aaggtatatg acttttcgga acctttcaga cggttggttt
    17041 tttcatcgat ttgccggacg ctctcaatac ccagcgattc cgccaccaac gtaagcgtac
    17101 cgttatctgg tgaaacgaag tactgaccgt ttttggtttt cagcactacc gatttacgct
    17161 ctgtaccaac acctggatcg accacgctaa caaacaccga accttgcggc cagtaactgg
    17221 cagtctgata caggcggtaa gcgccttccc agatgttata cggtggaatt tcgtgtgtta
    17281 aatcaaatat tttaagatta tggtcaacgc cgaaagcgac ccctttcatc gccgagacgg
    17341 cgccatcttt caggctaaaa tccgtttgta aaatcaaagc gttatccgca taagcggatg
    17401 atgaccccag acaggttaat aaaaataatg ttgcgagcgt ttttttcatt tttgtttccc
    17461 ttcatttatc tgatgatttt ccagctgtgt agccagcccc agtttgtctg gcctcctcga
    17521 gtgtacaatg tttaaccttt ctgctcgacg gaaatcatgg tgacaaaagg atagcgttgc
    17581 cacgggattg cctcgccttt catatacata tgtgaaattg tgccatcaag ataaagcagc
    17641 tgctcaacgt tcagtttcgc tttggcataa caggcaaaat cataaaaatt tgtcgcctgc
    17701 tggctcaaca aaaacacggc gttcccatgt ttattaatcc caacaccgtt acgaatttta
    17761 cgtgaggcga cgttgggatg aatacgcgga ttaattacac cgttttccat caacaatggc
    17821 cctgactgca ccgcaaactg aatctcttta ctggttttaa aggcatccag acgaacgatg
    17881 ccgactttat ctcccgcgac ataaaacact ccgccaggac gaataaagaa attcccttca
    17941 cctgaagcga gatttaacgc caccttctgc tgaccgtttt cgatgtacaa accgagcggc
    18001 gcatagcttt catcatagat gccgccgttc atcgccatct gcacctgacc ctgactatta
    18061 atatccgcca gcagagcatg taacgttccc cacgcttcgc cattggcttt ttgccagtac
    18121 attttcaccc gctctgtttg aggattaacg gtatacgcct gtacggtcag cgtcggatct
    18181 gagggtgcgc aatcatcagc ggcaacagca aacaagggaa gtaaggtgag ggtgagaaaa
    18241 atccgtttga gattcaaggt gatcattcct ttaccaatga atagccgatg cgccattata
    18301 ggccctgaat gtgggatttt tttatcctgt tagcgacctt gacgagtacc aaaaagcgcg
    18361 aagttcaact attgttctgt ggtgttctgt tgcgtgttga cggcaaaatt ttgctggcgt
    18421 aacatgcgcg cacgatcact ctaagaggac attcgccttg gacacaccca gtagatactg
    18481 gctcattatc ctgtcatcca ggatcaactc ctaaggctat ccctttttgc tgatagcctt
    18541 agcggttgtc agcgacctca atttttcccg tcgcgctgag tcaggctgtt taatggtctg
    18601 aaacccaatt tgtttctgtg tgcccaccga actgtccgat attttaagca ttgggagtcc
    18661 cggtcatgct gagcgcattt caactggaaa ataaccgact gacccggctg gaagtcgaag
    18721 agtcacaacc ccttgtaaat gcagtatgga ttgatcttgt cgaaccggac gacgacgagc
    18781 gactgcgcgt acaatctgaa cttggccaga gcctggcaac ccgcccggaa ctggaagaca
    18841 tcgaagcatc ggcccgtttc tttgaagacg atgacggcct gcatattcac tccttcttct
    18901 tctttgaaga tgcggaagat cacgccggta actccactgt ggcatttacc atccgtgatg
    18961 gtcgtctgtt tactctgcgt gagcgtgaac tgcccgcttt tcgtctgtat cgtatgcgtg
    19021 cccgtagcca gtcaatggtg gacggtaacg cctacgagct gctgctggat ctgttcgaaa
    19081 ccaaaatcga acagttggca gatgaaattg aaaatatcta cagcgacctg gagcagttga
    19141 gccgggtaat tatggaaggg catcagggcg atgagtacga cgaggcgctc tccacgctgg
    19201 cggaactgga agatatcggc tggaaagttc gcctgtgtct gatggatacc cagcgcgcgc
    19261 tcaacttcct ggtacgtaaa gcacgcttac cgggtggaca actggagcag gcgcgtgaaa
    19321 tcctgcgaga tatcgaatcc ctgctgccgc ataacgaatc cctgttccag aaggtgaact
    19381 tcctgatgca ggcggcgatg ggctttatca atatcgagca gaaccgcatc atcaaaatct
    19441 tctcggtggt atccgtggtg ttcctgccac cgacgctggt cgcttccagc tacgggatga
    19501 actttgagtt tatgccggaa ctgaagtgga gcttcggcta cccaggcgcg attatcttta
    19561 tgatcctcgc gggcctggca ccgtatctgt actttaagcg gaagaactgg ttgtaatata
    19621 aatgccgggt aaagattgtt ctttatccgg cttattgctg aaaattgtct ttttcaaagt
    19681 catgcgggtt gaacactcgc tctatcttat tttcctgggt tagttgatac tcattgttat
    19741 cagcaataaa aaaatgagta ttacggtaag gatattcatt aataaccatt aaatagtttt
    19801 ttacgatgca attatcattg ctcttaatcg tcgggaaaca gtagagatct tcatacggcc
    19861 cttcgatata ttttttatag tcagtgaagc cattaccgat atcaataaca taccaggtat
    19921 gccaactatc ttctggtagg ggattttgac ttttaatttc atcctgatgc ttcagatacc
    19981 aatttatttt atcgctatca gtccatggca gatgatcaac gaccagaaag actgaattgc
    20041 cagcatcaga gtatgtatac aaaatctgta caggacgcaa tgtccataa
//
LOCUS       DAZFFE010000086        20022 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFFE010000086 DAZFFE010000000
VERSION     DAZFFE010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.086"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..784
                     /locus_tag="ACHJ69_003401"
     CDS             <1..784
                     /locus_tag="ACHJ69_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003834018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5016321.1"
                     /translation="HELATETRKDYRKYGSKVNVVFGKMKPDNIKPEHIRKYMDKRGV
                     KSRVQANREKAFMSRVFRWAYERGKVKMNPCQGVKQFKEQARTRYISDREYDALYSVA
                     SIPVKVAMELAYLCCARQGDILDLKKSQLLSEGILIQQSKTAVSQIKAWTKRLEDAVN
                     LADSIPLNSGMVSIFVIHQPSGLRYTRDAFNAQWMKTKKLAAEKYPDLDFQFTFHDLK
                     AKGISDLEGTLSEKQEISGHKNASQTARYNRRISVVPVVGGQ"
     gene            complement(910..996)
                     /locus_tag="ACHJ69_003402"
     tRNA            complement(910..996)
                     /locus_tag="ACHJ69_003402"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(960..962),aa:Leu,seq:taa)
     gene            complement(1009..1082)
                     /locus_tag="ACHJ69_003403"
     tRNA            complement(1009..1082)
                     /locus_tag="ACHJ69_003403"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1048..1050),aa:Cys,seq:gca)
     gene            complement(1136..1211)
                     /locus_tag="ACHJ69_003404"
     tRNA            complement(1136..1211)
                     /locus_tag="ACHJ69_003404"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1176..1178),aa:Gly,seq:gcc)
     gene            complement(1363..1911)
                     /gene="pgsA"
                     /locus_tag="ACHJ69_003405"
     CDS             complement(1363..1911)
                     /gene="pgsA"
                     /locus_tag="ACHJ69_003405"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO5016322.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(1968..3800)
                     /gene="uvrC"
                     /locus_tag="ACHJ69_003406"
     CDS             complement(1968..3800)
                     /gene="uvrC"
                     /locus_tag="ACHJ69_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO5016323.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGIGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(3797..4453)
                     /gene="uvrY"
                     /locus_tag="ACHJ69_003407"
     CDS             complement(3797..4453)
                     /gene="uvrY"
                     /locus_tag="ACHJ69_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO5016324.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            4749..4925
                     /gene="yecU"
                     /locus_tag="ACHJ69_003408"
     CDS             4749..4925
                     /gene="yecU"
                     /locus_tag="ACHJ69_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO5016325.1"
                     /translation="MIKIFIGHYINVFYSTVDITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            4912..5136
                     /gene="yecF"
                     /locus_tag="ACHJ69_003409"
     CDS             4912..5136
                     /gene="yecF"
                     /locus_tag="ACHJ69_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO5016326.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(5204..5926)
                     /gene="sdiA"
                     /locus_tag="ACHJ69_003410"
     CDS             complement(5204..5926)
                     /gene="sdiA"
                     /locus_tag="ACHJ69_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO5016327.1"
                     /translation="MQDTDFFSWLRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(6156..6908)
                     /gene="tcyN"
                     /locus_tag="ACHJ69_003411"
     CDS             complement(6156..6908)
                     /gene="tcyN"
                     /locus_tag="ACHJ69_003411"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO5016328.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGVAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(6905..7573)
                     /gene="tcyL"
                     /locus_tag="ACHJ69_003412"
     CDS             complement(6905..7573)
                     /gene="tcyL"
                     /locus_tag="ACHJ69_003412"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO5016329.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(7588..8574)
                     /gene="dcyD"
                     /locus_tag="ACHJ69_003413"
     CDS             complement(7588..8574)
                     /gene="dcyD"
                     /locus_tag="ACHJ69_003413"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO5016330.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREDADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNTQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(8679..9479)
                     /gene="tcyJ"
                     /locus_tag="ACHJ69_003414"
     CDS             complement(8679..9479)
                     /gene="tcyJ"
                     /locus_tag="ACHJ69_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5016331.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(9567..10118)
                     /gene="fliZ"
                     /locus_tag="ACHJ69_003415"
     CDS             complement(9567..10118)
                     /gene="fliZ"
                     /locus_tag="ACHJ69_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO5016332.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPSTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSQPASDIY"
     gene            complement(10164..10883)
                     /gene="fliA"
                     /locus_tag="ACHJ69_003416"
     CDS             complement(10164..10883)
                     /gene="fliA"
                     /locus_tag="ACHJ69_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO5016333.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(11203..12960)
                     /locus_tag="ACHJ69_003417"
     CDS             complement(11203..12960)
                     /locus_tag="ACHJ69_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFO5016334.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGETITIDL
                     KKIDSDTLGLNGFNVNGKGTITNKAATVSDLTSAGAKLNTTTGLYDLKTENTLLTTDA
                     AFDKLGNGDKVTVGGVDYTYNAKSGDFTTTKSTAGTGVDAAAQATDSAKKRDALAATL
                     HADVGKSVNGSYTTKDGTVSFETDSAGNITIGGSQAYVDDAGNLTTNNAGSAAKADMK
                     ALLKAASEGSDGASLTFNGTEYTIAKATPATTSPVAPLIPGGITYQATVSKDVVLSET
                     KAAAATSSITFNSGVLSKTIGFTAGESSDAAKSYVDDKGGITNVADYTVSYSVNKDNG
                     SVTVAGYASATDTNKDYAPAIGTAVNVNSAGKITTETTSAGSATTNPLAALDDAISSI
                     DKFRSSLGAIQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGN
                     SVLAKANQVPQQVLSLLQG"
     gene            13226..14632
                     /gene="fliD"
                     /locus_tag="ACHJ69_003418"
     CDS             13226..14632
                     /gene="fliD"
                     /locus_tag="ACHJ69_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO5016335.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQNSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            14657..15067
                     /gene="fliS"
                     /locus_tag="ACHJ69_003419"
     CDS             14657..15067
                     /gene="fliS"
                     /locus_tag="ACHJ69_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO5016336.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEKSKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            15067..15432
                     /gene="fliT"
                     /locus_tag="ACHJ69_003420"
     CDS             15067..15432
                     /gene="fliT"
                     /locus_tag="ACHJ69_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO5016337.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            15510..16997
                     /gene="amyA"
                     /locus_tag="ACHJ69_003421"
     CDS             15510..16997
                     /gene="amyA"
                     /locus_tag="ACHJ69_003421"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO5016338.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(17031..17444)
                     /gene="yedD"
                     /locus_tag="ACHJ69_003422"
     CDS             complement(17031..17444)
                     /gene="yedD"
                     /locus_tag="ACHJ69_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO5016339.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLTGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            17631..18836
                     /gene="yedE"
                     /locus_tag="ACHJ69_003423"
     CDS             17631..18836
                     /gene="yedE"
                     /locus_tag="ACHJ69_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO5016340.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQIAKEIA"
     gene            18833..19066
                     /gene="yedF"
                     /locus_tag="ACHJ69_003424"
     CDS             18833..19066
                     /gene="yedF"
                     /locus_tag="ACHJ69_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO5016341.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            19175..19842
                     /locus_tag="ACHJ69_003425"
                     /pseudo
     CDS             19175..19842
                     /locus_tag="ACHJ69_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
BASE COUNT         5079 a         5110 c         4836 g         4997 t
ORIGIN      
        1 ccacgagtta gcaactgaaa cacgtaaaga ctacagaaaa tacggttcaa aggtaaatgt
       61 tgtcttcggg aaaatgaagc cggataacat caagccagag cacatcagaa aatatatgga
      121 taaacgaggt gttaagagca gggttcaggc aaaccgcgag aaagcattta tgtccagggt
      181 gtttcggtgg gcatacgaac gaggaaaggt gaagatgaat ccttgccagg gcgtaaagca
      241 atttaaagag caggctcgga cccgttatat tagcgatagg gaatatgatg cactttatag
      301 cgttgcttct attcccgtaa aagttgcaat ggagttggca tatctatgtt gcgcccgcca
      361 gggagacatt cttgacctca aaaagagtca attattgagc gaaggaattt taattcagca
      421 gagtaaaacc gcagttagcc agatcaaggc ctggacaaaa cgtctggagg atgccgttaa
      481 tcttgctgac agcattcctc ttaacagcgg gatggtaagt atttttgtca tccaccagcc
      541 atcaggtctg cgatacacac gcgatgcttt taatgcgcaa tggatgaaga ctaaaaaact
      601 tgctgctgaa aaatatccag accttgattt ccagttcact tttcatgatc taaaagccaa
      661 aggaatttct gatcttgaag gtacgttaag tgagaagcag gaaatttcag gtcataaaaa
      721 cgcctcgcaa acagcgagat ataatcgaag aatttcagtt gttccggtcg tcggggggca
      781 gtaatgccct ctttttatgg cgaagctgaa tggcgaaaca atggtgaaca tggagtacaa
      841 aaacaaaaaa accacccgta ggtggtttca cgacactgct tattgctttg attattctgt
      901 tctttcccat ggtacccgga gtgggacttg aacccacaca gcgcgaacgc cgagggattt
      961 taaatccctt gtgtctaccg attccaccat ccgggctcgg gaagaaagtg gaggcgcgtt
     1021 ccggagtcga accggactag acggatttgc aatccgctac ataaccgctt tgttaacgcg
     1081 ccaaattctt caggcctttc agccagacat ccgctgccgc cgatgtcttt taaactggag
     1141 cgggaaacga gactcgaact cgcgaccccg accttggcaa ggtcgtgctc taccaactga
     1201 gctattcccg cattcatcaa gcaatcagtt aatcacttga ttttattatc gtctggcaat
     1261 cagtgccgcc gttcgatgcg ttgcattcta cttacctggc gcgatgagtc aacgatattt
     1321 ttcaccactt ttgatcgttt gctgaaaatt gcgccgaaac gatcactgat caagcaaatc
     1381 tgcacgcgca gcgctcaaat attgcaacat tgaccacaga gtcagtaccg cagccacaaa
     1441 gaaaagtgca ataccggcgt actcaaccca aatgttcgga cgccacagca gccaggctaa
     1501 cgccaccatc tgggcagtgg ttttcacttt cccaatccag gagacagcca cgctactgcg
     1561 tttacccaac tccgccatcc attcgcgtag cgcagaaata ataatttcac gggcgatcat
     1621 cgttgctgcc ggtaaggtca cccaccagct gtggtaatgc tcggttacca gcaccatggc
     1681 gatagccacg agaactttat ctgcgacagg gtcaaggaaa gcaccaaacc gggtactctg
     1741 attccagcgg cgtgccagaa aaccatcgaa ccagtcagtc accgccgcga cgcagaaaat
     1801 gagcgcggcg gcaaacggcg accaggtgac aggcagataa aagaccaata caaagaatgg
     1861 gataaggatg acacggaaca gtgtaagcaa cgtagggata ttaaattgca taatgacggg
     1921 taactatctg ttgtcagtaa gattacccct atgttgctac agagacatca atgtttcaac
     1981 gaccagaaga tcttttctgc cagaccttgc gaaatacccg gcacttttgc aatttcctca
     2041 acgctggcgt tacgtaaacc ttgcaaacca cccatatatt tcaacaacat ttgccgacgt
     2101 tttggcccga tgccttcaat ggtttccagg gaactggtat ttttgacctt cgcccgtttt
     2161 ttacggtgcc cgccaatcgc gtgatcgtgt gattcatcgc gaatatgctg gataacatgc
     2221 agcgcgggag aatctggcgg caaactaaat ccctcacctt ccggctcaaa gaacagcgtt
     2281 tccagcccag ccttacgatc tgctcctttg gcaacgccaa gtagcagcgg atgattttta
     2341 tcccatgaga catccagttc ggcgaagaca tttttcgcct gcgcaagctg gcctttgccg
     2401 ccgtcgataa ggatcacatc cgggatctta ctgtcgtcaa tggctttacc ataacgccga
     2461 cgcagcacct gattcatcgc cgcataatca tcgcccggtg tgatgccagt aatgttatag
     2521 cgccgatact ccgcacgcag cgggccgtta gcatcaaaca ccacacagga agcgacggtt
     2581 tgttcaccca tggtatggct gatgtcaaag cactccatcc gcttcacttc cggcaatttc
     2641 aacacgctgg caagcgcggt cagccgctgg tgaacggtag attgctgcga aagtttgctg
     2701 gttaaggccg tcgccgcatt ggtacgcgcg agtttcagat aacgcgctct atcgccacga
     2761 ggtttggttt gaacattaat cttgcgtccc gccagttctg aaagggaatc ggcgagcagc
     2821 gttttatcgc taagattaaa atcgagcagg atctcacccg gtaaggtgcg catctggctg
     2881 ccttgtaaat agaactgacc tacgaaggtt tccaccacct cgctcagttc cgtaccgcca
     2941 ggcactttcg ggaaatagct gcggctgccg agcactttgc cctgacgaat gaacaatacg
     3001 tggacacaag ccatacccgc atcgaacgcc actccaataa cgtcaaggtc gtcgccggta
     3061 ttggaaacaa actgtttttc ggtgacacgt cgcaccgcct gaatttggtc gcgaatccgc
     3121 gccgcttctt caaactccag attctggctg gcagtttcca tacgactaat gagttgcgta
     3181 agcacctgat catctttgcc agacaaaaac aggcgcacat actcgacctg ctgagcgtat
     3241 tcttcttcac tcaccagtcc ttcaacgcac ggccccagac agcgccctat ctgatattgc
     3301 agacacggac gcgagcggtt gcgataaaca ctgttttcgc actggcgaat ggggaaaatc
     3361 ttttgcagta gcgccagtgt ttcacgtacg gcatagccat tcgggaacgg gccgaaatat
     3421 tcacctttgg catgcttcgc tccacgatgc atcgccagac gcgggtgggt atcgccactc
     3481 aggaagataa aaggatatga tttatcatcg cgtagcaaaa cgttgtaacg cggctgataa
     3541 agtttgatgt agttgtgttc cagtaacagc gcttcggttt ctgtatgagt aaccgttaca
     3601 tcaatttgct ggatctgggc gaccagcgct tcggttttgc gcgaagcgag gttgctacgg
     3661 aaatagctgg aaagccgttt tttcaggtct ttcgctttgc cgacatagat aaccgtacca
     3721 ccagcatcgt acatgcgata aacgcctggc tggctggtta cggtttttaa aaacgctttt
     3781 gcgtcaaact gatcactcac tgacttgata atgtctccgc attacacaga ccatggcgaa
     3841 ttgccaggtg agtcagctca acatcgccat gaatgtttag tttactgaac atacgatagc
     3901 ggtagctgtt caccgttttc ggactgagat tgagctgttc tgagatctca ttgaccttct
     3961 ggcccttggt gatcatcagc ataatctgca attcacgttc agacaaactg gcaaatgggc
     4021 tttctgtttt ttctggttcg atctggctta acgccatttg ttgagcgata tcagaagcaa
     4081 tgtaacgctg ccctgaatag acagaacgaa tcgcactcac gacttcctgc ggagccgcgc
     4141 ctttgctgag gtagcccgca gctccggcct gcatgacttt cgctggtaaa gggttttctg
     4201 tatggacggt aagcatgatg attttgacat cagctgtgga acgcgcgatt ttacgcgtcg
     4261 cctcaagacc gccaatgccc ggcatactca tgtccattag caccacgtca acggcatttg
     4321 cccggcacca tttaacggcg tcttcaccgc acgatgcctc accgacgact tttataccct
     4381 ttatatcttc cagaatgcgt cgtatccctg cgcgcaccag ttcgtggtca tcaacaagta
     4441 gaacgttgat caaaggaata tctccagaaa tagggataac gctactgata gttagtcatt
     4501 cgtatattag cggtttttat tgcaactttg aaacgttaaa aatggtgggt attcgatttt
     4561 tctctcgttt ttgtcaattc gattgtccac agttatggtc acgcccgtca ccaacggctt
     4621 ccgggatcgt ttcacagtaa gcccggcgat gaaatgtgac aaaaattgac tttattcagc
     4681 aaaaatgaaa atcagcccgc tgaatatgta acattaatta acccgaacaa tacgggcaaa
     4741 aaacattcat gataaaaata tttattggtc attatattaa cgtattttat agtactgttg
     4801 atatcacgct caaaaaacaa ccactgctat ttttagcaaa gcttatggta tactccgccg
     4861 ccttaacatt tttcaccgca aattttcatt gcaacatgac gaggaaaata aatgagtacg
     4921 cctgattttt ctactgccga gaataatcaa gaactggcaa atgaagtctc ctgcctgaaa
     4981 gcgatgctga cgctgatgct gcaggcgatg ggacaagctg acgcgggccg cgtgatgtta
     5041 aagatggaaa aacagcttgc gctgatcgaa gacgaaaccc aggctgcagt attttccaaa
     5101 acggttaagc aaattaaaca agcctaccgt cagtaatata aaaccggctg agagcgtgcc
     5161 tttcagccgg tttttgcatc tggcacgcag aacagaaaag agatcaaatt aagccagtag
     5221 cggccgcgta acaggcaacc tgcgtcttat tcggtgcatt gatttttttc tgcatgtttt
     5281 tctgatggaa attgaccgta ttctcagaga ttgacaaaat catcgctatc tctgctgatg
     5341 tcttaccttc cgccgtccat ttcagaattt ctttttcgcg cttgctgaaa ttcatctctg
     5401 gcgtcatcac tatttcatca tttaaacgca tcagagccat cagactttcg cgcaccagta
     5461 gctgcatttt taattgcagt tcatcactaa gaatgggtat ttcgcgcgtg ctgcaacggg
     5521 aaaaggacaa aaagcccaac gcccgatttg gcagcattaa atactgagtg acaccgcggc
     5581 gtaaaccatg tgcgcgcgca gcttcccata acggctgtgc ttcgctgaat aagtcatcat
     5641 tccacattaa atggccctga ctaaagtttt cagggttcag caccggatca attgcgagaa
     5701 aattttttgc ctgataataa ctaacccacg actcagggta attggtatag aaagccactt
     5761 taggtcgcgt gaatggtacc gggtggcgaa cacataacga atagtaatcg tactccagct
     5821 gctgagcctg aagctctatt tcatggtaga cctcttctgc ggcctccatc ctctgaaaac
     5881 gcaacaacat cgtgcgaagc cagctgaaaa aatccgtatc ctgcatagta aaccgcaacg
     5941 cccctgagag tgagtatcat ttataatgat aattcaaact atcacataaa taagatttat
     6001 atataattta tattattcag gcaatgaatt acttttgcaa gccatcgcat tctcttatgt
     6061 tattaatgag ttatgctgat ttgttaagca gttttatcac gtttgaaatg gcgtcccgcc
     6121 ccgactggtg aatcgtcggg gctgattttt tcttattatt gcagcagaaa cttctccagg
     6181 aactggcggg tgcgaggctg ctgaggatca gcaaataacg cttttgctac cccctgctcg
     6241 actatccgcc cctggtccat aaaaatcgcc cggtccgcaa catcccgggc aaagctcatt
     6301 tcgtgcgtca caatcaccat cgtgcgcttt tcctgcgcca gctgacgaat ggtgttcagg
     6361 acttcgccca ccagctctgg atccagcgcc gacgttggct catcaaacaa aatcacttca
     6421 ggacgcattg ccagagcacg cgcaatcgca acgcgctgct gttgaccgcc agacaaacga
     6481 cgtggataac tggtttcttt accggccagc ccaactttcg ccagcagctc gcgagcgcgc
     6541 gccgtggcct cttctttcgg ttcgcctttg acgatcaccg gcccttcaat aatgttttcc
     6601 agcaccgtac gatgcggaaa cagattaaag ttctggaaga caaacccgac gtgctgacgc
     6661 aactggcgaa tcagcgattt ttgctgactt aatgaacgtg cagtatcaat agtgatatcg
     6721 ccaacggtga tggttcccgc ttccggttgt tccagcagat ttatgctgcg tagcaacgtg
     6781 gttttgccgg aaccactcgg accgataata gccactactt cgccaggctt cacctcaagg
     6841 tcgataccat gcagcaccgt ctgaccgtgg aattttttca ccaggttctt aacttcaatg
     6901 gcactcattt gggttccctc tcctggcgat taagttgatt ctcaaaatgg ttctgcaacg
     6961 tcgataacac agtcgccata atccagtaga tgagcgaagc cgccaggtac atggtgaaca
     7021 cctccagcgt gcgcgaggta atcaactgcg cctgacggaa cagctccggc acctggattg
     7081 tcgcggccag cgacgtatct tttaccaggc tgataaagct gttcgacagc ggcggcaacg
     7141 ccacccgcgc cgcctgtggc aaaatcgcgc gacgcatggt ctgccatggc gtcataccaa
     7201 tactggctgc agcttcccac tgacctttat caatagaaga aatggcggcg cgcagcgttt
     7261 cggcagcata ggcggcagtg ttcagcgaca gaccaatcat tgctgacgga atgggatcta
     7321 attcgatacc aaactgtggc agtccgtaat agatcataaa cagttgtgcg atgagcggcg
     7381 taccacgaaa aatagagata taaaaacgcg ccaaccagcg cacgggccag attggcgaca
     7441 ggcgcatcag cgcgagaata aacccgagca gtaagccaaa aaacatgccg ccgatactga
     7501 gttgcagcgt atacccagcc cctttcagca ggaacggcaa ggaatcaata accagttgta
     7561 tactttcttg catgagcgtt cccggagcta aacgtgggga tgataggcga acagcgcagg
     7621 cgcaccgccg gtatgaataa acaaaatcgg cccttcatct ttgaagcgtt tctgactgat
     7681 accgtcaatc agccccgcca tcgcttttcc ggtatacaca ggatcaagca gaatgccttc
     7741 cagccgcgcc agcaatttca ctgcttccat gccttcgtcg ttcggcacgc cgtagccagg
     7801 tgcaaaatag tcatcccaga gtaaaatttc cgctgatgcg gtcagctcca gttctttcgc
     7861 aatcgcctgt tggaggttaa ccactttcgg caattgatcg gcaacagaac gcgacacagt
     7921 cacgccaatc agttcgcttt caggcatcag gtgttccagc ccaacagcca gtccggcgtg
     7981 agttccggca ctgcccgatg cgaccaccac cgacgaaata ttaaccgccc cttcacactg
     8041 ttgcgcgatt tccagcgcac tctccacata acccagcgcg cccaaagcat tagaaccgcc
     8101 aaccggaatg acatacgggc gaaagccttg tgcttcgact cgcgtcgcca gctcttccag
     8161 ttgggtattg ggatcggtca gtgcgtcgca catttcaatc tgggtattga acagatccag
     8221 caacaaacga ttgccgttgg ttaaatagtt ttccgcggtt gtaccaatag gattttccag
     8281 cagcgccacg cagtgcagac cgagtttcgc agcgactgcg gcagtctggc gcacatggtt
     8341 agactggatc gccccggcag taatcagcgt atcggcatct tcacgcagag catctgccgc
     8401 gagaaattcc agcttacgta atttattgcc gcccattgcc atgggagtga cgtcatcccg
     8461 tttgatgaaa atttcccgtc caagataatc agaaaagcgc ggcagatatt cgagcggcgt
     8521 tggcgcgccg ataaactcca gccgtggaaa acgggttaaa ttatgcagtg gcataacagc
     8581 ctccgatgtg tgttgttgtg attttcttat tatgcacgct gaaaacgcgt aaataaaaaa
     8641 ggcgctagtg aaagcgccct tttttgtcat tatgctgatt atttggtcac atcagcacca
     8701 aaccattttt cggaaagcgc ttgcagagtg ccgtcttttt gcatttccgc aattgcatca
     8761 ttcactgctt tcagcaggtc ttcatttcct ttacgcaacg ccacgccaga ctcctgacgg
     8821 gagaatgctt caccggttac tgccagcgta tcgttggttt tcttcaccag atccagcgcc
     8881 gccagacgat caacgaggat cgcatcgata cggcctacgc gcagatcctg atatttggtc
     8941 gggtcatcat cataggtacg cacatccacg ccctgaacat tctgacgcag ccactcttca
     9001 tagttggtgc ccagaccgac acccactttt ttgcctttca gatcatcagc cgttttaatg
     9061 gtgccttcgt tacctttttt caccagcgcc tgaataccgg aaatggtgta cggggtagag
     9121 aaatcgtatt ttttcttgcg ctcgtcagaa atggtgacct ggttaatcac cacatcaatg
     9181 cgtttagagt ccagcgacgc cagcataccg tcccatttag tcggttttag tgacgcctca
     9241 acgccaagat gttttgccag ctgttgggca aattccactt caaaaccggt taatttgccg
     9301 tcatccccct gaaaactgaa cggcggataa gttccttcca gccctaccag cagcgtgccg
     9361 cgctctttga ctttattaag cagaccttca tctgcaaaac ttttaacgct catacccgca
     9421 accagcgcca cggccatcac acccatcaat gcctgacgtc ccagatgtgc taatttcata
     9481 ttcaccccga atgttgttat gtctgtttgc agtgtagagc cattgtttgt aaacacaaaa
     9541 acaactccgc tacatcttat tcttatttaa tatatatcag aagcaggttg gctggaggat
     9601 ttctgcacaa gtccggtaca cgtttgacgc tgatagtgtt gatacttccg taacgcgatg
     9661 cggtaattgt tggtgctggt ggagggcagc cacggggcga ggatttcatc aaggaaaccg
     9721 tcctgcagct ggtcgaggga aatattttgc tcgtgtagat gattccccaa acggcgcaga
     9781 cgaacgacat attcacgcac cgtaccaggg ctcatttcag tttgctcaaa aagaaattgc
     9841 ttaaagccga taatatcgaa aaaatcactc tgcgttttgc aatgtaaatc accgcaaaat
     9901 cggcacaatg ccgcccatga ttgttgttcc acttgccagc cattttcatc aagcagcgtg
     9961 tccaggcggg aaatctcgat tttattcact attttgccgt cgcgaactaa ggtaatgcga
    10021 tcgagtaatt tacggcaatg cgcgcaatgg gtctggctgt gtttaaagtc tttaagatag
    10081 cggcttaatg gccgtctttt caggtgctgc accatcatta agaactcctg gtagtcaaaa
    10141 ttaaagtgcg gcatttactg acgttataac ttacccagtt tagtgcgtaa ccgtttaata
    10201 gcctggctgt gtaactgact gacccgcgat tccccgacct ccagcaccgc accaatctct
    10261 ttgagattca gctcttcctg ataatagagg gttaatacca gtttttcgcg ctccggcaac
    10321 gtttcgatgg cttccatcac ccgctggcgc agattactgt ccagcagttg ttgtagcggg
    10381 ttttctcgct gatgatcatc agtaaccagt tcgatgctat cgccgtgctc ttcgcgccac
    10441 tcatcgtagg agaagagctg gctgttattg gtgtcgagca acatttggcg ataatcggca
    10501 atatcgatcc ctaaacgttc cgctacctca gtttccgtgg cgttgcggcc aagttcctgc
    10561 tccagttgcc ctattgcctg tgccacttca cgcgcgttgc gtcgcacgct gcgcggcacc
    10621 cagtcacggc tgcgaagttc atccagcata gcgccacgta tacgctgcac tgcgtaagtt
    10681 gtaaatgccg ttccttgtag ggcgtcatag cgttcgacgg cattaagtaa cccaatgccg
    10741 cccgcctgta gcagatcgtc aagttccacg ctcgcgggca gtcgaacctg caggcgcaat
    10801 gcttcgtgac gcaccagcgg gacataacgc tgccacagcg agtgtttatc cattacacct
    10861 tcagcggtat agagtgaatt cacgataaac agccctgcgt tatatgagtt atcggcatga
    10921 ttatccgttt ctgcagggtt tttaatcggg cgattagtgg gtgaaatgag gggttatttg
    10981 ggggttaccg gtaaattgcg ggcagaaaaa aaccccgccg ttggcgggga agcacgttgc
    11041 tgacaaatta cgcttcatgt tgccggatgc ggcgtaacgc cttatccggc ctacaaaaat
    11101 ctgcaaatta aataaattgc aattcacctt gtaggcctga taagcacagc gcaccaggca
    11161 atttggcgtt gccgtcagtc tcagttaatc aggttacggc gattaaccct gcagcagaga
    11221 cagaacctgc tgcggtacct ggttggcttt tgccagcacg gagttaccgg cctgctggat
    11281 aatctgcgct ttcgacatgt tggacacttc ggtcgcatag tcggcgtcct gaatacggga
    11341 ctgcgcttca gacaggttgg tagtggtgtt gttcaggttg gtgactgcgg aatccagacg
    11401 gttctggata gcacccaggg aagaacggaa tttgtcgatg gagctgatag cgtcgtccag
    11461 ggcagcaagc gggttggtcg ttgcagaacc agcactggta gtctcagtag tgattttacc
    11521 cgcggagttc acatttacag cagtaccaat tgctggagca taatctttat tggtatcagt
    11581 cgctgaagca tacccggcaa cagtcacaga gccgttatcc ttgttaacgc tgtaagagac
    11641 tgtatagtcg gcaacgttag taataccacc tttatcatcc acataagact tcgcagcatc
    11701 actggattca cccgcggtaa acccaatagt tttgctcagt acaccggaat taaaggtaat
    11761 tgaagatgtc gcggcagccg ctttggtttc gctcaatact acatctttac tcactgtagc
    11821 ctgataagta atcccaccag ggattaacgg agctactgga gaggttgtcg caggagttgc
    11881 ttttgcgata gtatattcag tgccattgaa tgtcagagaa gcaccgtcac taccttcgct
    11941 cgcggcttta agcagcgctt tcatatcagc tttagctgcg ctaccagcgt tgttagtcgt
    12001 caagttgcct gcatcgtcta cgtatgcctg gcttccaccg atggtgatat tacctgctga
    12061 atccgtttcg aaagaaacag taccatcttt tgtggtgtaa gaaccattaa cagatttacc
    12121 cacatcagca tgaagggtgg cagctaacgc atcacgtttt ttagctgaat cagtagcctg
    12181 cgccgcggcg tctacacccg taccagcagt agatttggtg gtagtaaaat caccagattt
    12241 agcgttgtaa gtataatcta cgccgccaac ggtgacttta tcgccattcc ctaatttatc
    12301 gaatgcagca tcggtagtta acaaggtatt ttcggttttc agatcataaa gacctgtcgt
    12361 ggtgtttaac ttcgcgccag cagaagttaa atcacttacc gttgcagctt tgttggtaat
    12421 agtaccttta ccatttacgt taaaaccatt cagacccaga gtatcagaat cgattttctt
    12481 caggtcgata gtgatagttt caccgtcatt cgcaccaacc tgaattttca tcgaaccgtc
    12541 ttttgccagt acgttcacgc cgttgaactg ggtctggccg gatacgcggt caatttcatc
    12601 aagacgggat ttgatttcgt cctgaatgga gtccagatcc gaatcagagt tagttccggt
    12661 agaagcctga accgtcagct cacggatacg ctgtaagttg ttgttgattt cagacagcgc
    12721 gccttcggtg gtctgtgcaa cagaaatacc gtcgttggcg ttacgtgcag cctgagtcag
    12781 gcctttaatg ttagaagtaa aacggttagc aatcgcctga cccgcggcgt catccttcgc
    12841 gctgttaata cgcaagccag aagacagacg ctcgatagaa ctcgacagcg cagactggtt
    12901 cttgttgata ttattttgag tgatcagcga gaggctgttg gtattaatga cttgtgccat
    12961 gattcgttat cctatattgc aagtcgttga ttacgttttg ggtttccacc cgtcggctca
    13021 atcgccgtca accctgttat cgtctgtcgt aaaacaacct ttagaatttt tttcacaaac
    13081 agccattttt tgttagtcga cgaaatactc ttttctctgc cccttattcc cgctattaaa
    13141 aaaaacaatt aaacgtaaac tttgcgcaat tcaggccgat aaccccggta ttcgttttac
    13201 gtgtcgaaag ataaaaggaa atcgcatggc aagtatttca tcgctgggag tcgggtcagg
    13261 tctggattta agttcaatcc ttgatagcct caccgccgcg caaaaagcga cgctaacccc
    13321 tatttcaaat cagcaatcgt cgtttaccgc taaacttagc gcctacggta cgctgaaaag
    13381 cgcgctgaca actttccaga ccgccaatac tgcattgtct aaagccgatc ttttttccgc
    13441 taccagcacc accagcagca ccaccgcgtt cagtgccacc accgcgggta acgccatcgc
    13501 cgggaaatac accatcagcg tcacccatct ggcgcaggcg caaaccctga caacgcgcac
    13561 caccagagac gatacgaaaa cggcgatcgc caccagcgac agtaaactca ccattcaaca
    13621 aggcggcgac aaagatccga ttaccattga tatcagcgcg gctaactcgt cgttaagcgg
    13681 gatccgtgat gccatcaaca acgcaaaagc gggcgtaagc gcaagcatca ttaacgtggg
    13741 taacggtgaa tatcgtctgt cagtcacatc aaatgacacc ggccttgaca atgcgatgac
    13801 gctctcggtt agcggtgatg atgcgctaca aagttttatg ggctatgacg ccagtgccag
    13861 cagcaacggc atggaggtct cggtcgccgc ccagaatgca cagctgacgg tcaataacgt
    13921 cgccattgag aacagcagca acaccatcag cgacgcgctg gaaaacatca ccctgaacct
    13981 gaacgatgtc accacaggca accagacgct gaccatcact caggacacct ccaaagcgca
    14041 aacggcgatt aaagactggg tgaatgccta taactcgcta atagatacct ttagcagcct
    14101 gaccaaatac accgccgtag atgcgggagc tgatagccag aattctagca atggcgcact
    14161 actcggcgac tccacgctgc gaacgattca gacgcagttg aaatcgatgc tgagtaatac
    14221 cgtcagttct tccaactata aaacgctggc gcagattggt atcacgaccg atcccagcga
    14281 tggcaaactg gaactggatg ccgacaaact caccgctgca ttgaaaaaag atgccagcgg
    14341 cgtaggtgca ttgattgttg gcgatggtaa aaaaaccggc atcacgacca ccatcggcag
    14401 caacctgacc agttggcttt cgacaacggg cattattaaa gccgctaccg atggcgttag
    14461 taagaccctg aataagttaa ctaaagacta caacgccgcc agcgatcgca ttgatgcgca
    14521 ggtcgcgcgc tataaagaac aatttaccca actggacgtt ttaatgacct cgttaaacag
    14581 caccagcagc tacttaacgc agcagttcga aaacaacagt aattccaagt aatcaatatt
    14641 catcgggaga caggtcatgt acgcggcaaa aggcacccag gcctatgcac aaattggcgt
    14701 cgaaagcgcc gtaatgagcg ccagccaaca gcagttggtc accatgctat ttgatggagt
    14761 gttgagcgca ctggttagag cgcgcctgtt tatgcaggac aacaatcagc aaggcaaagg
    14821 cgtctctttg tcaaaagcga tcaacatcat tgaaaacgga ctgcgggtga gtcttgatga
    14881 aaagagcaaa gacgaattaa cccaaaactt aattgctctt tatagctata tggtcaggcg
    14941 tttgctgcaa gccaatttac gcaacgatgt ctccgcagtc gaagaggtgg aagcattaat
    15001 gcgtaatatt gccgatgcct ggaaagagtc tttactctcc ccttctttga ttcaggaccc
    15061 agtctgatga acaatgcacc gcatttgtat ttcgcctggc aacaactcgt cgaaaaaagc
    15121 cagctcatgt tacgcctggc aacggaagaa caatgggacg aactcatcgc cagcgaaatg
    15181 gcgtatgtga atgcggtgca ggagatcgca cacctgactg aagaggttgc cccgtccact
    15241 acgatgcagg agcagctccg cccgatgctg cacctgattc tcgacaacga aagcaaggta
    15301 aagcagttat tacagattcg gatggatgaa ctggcgaaac tggtcggtca gtcatcggtg
    15361 caaaaatcgg tgttaagtgc ctatggcgat cagggcggct ttgtgctggc tccgcaggat
    15421 aacctctttt gaatcttaat gagccgatgg ctcgcgaata atccgattac ggctacgctt
    15481 ctaatattcc ccttgaatgg agtcgaagaa tgcgtaatcc cacgctgtta caatgttttc
    15541 actggtatta cccggaaggc ggtaagctct ggcctgaact ggccgagcgc gcagacggtt
    15601 ttaatgatat tggtatcaat atggtctggt tgccgcccgc ctataaaggc gcatcgggcg
    15661 gctattcggt cggctacgac tcctatgatt tatttgatct aggtgagttt gatcagaaag
    15721 gcagcatccc tactaaatat ggtgataaag cacaactgct ggccgccatt gacgctctga
    15781 aacgtaatga cattgcggtg ctgttggacg tggtagtcaa ccacaaaatg ggcgcggatg
    15841 aaaaagaagc tattcgcgtg cagcgtgtaa atgctgacga ccgtacgcaa attgatgaag
    15901 aaatcattga gtgcgaaggc tggacgcgtt acaccttccc cgcccgtgcc gggcaatact
    15961 cgcagtttat ctgggatttc aaatgtttta gcggtatcga ccatatcgaa aatcctgatg
    16021 aagatggcat atttaaaatt gttaacgact acaccggcga aggctggaac gatcaggttg
    16081 atgatgaact cggtaatttc gattatctga tgggcgagaa tatcgatttt cgcaatcatg
    16141 ccgtgacgga agagattaaa tactgggcgc gctgggtgat ggaacaaacg caatgcgacg
    16201 gttttcgtct tgatgcggtc aaacatattc cggcgtggtt ttataaagag tggatcgaac
    16261 acgtacaaga agttgcacca aagccgctat ttattgtggc tgaatactgg tcgcatgaag
    16321 tcgataaact gcaaacgtat attgatcagg tggaaggcaa aaccatgctg ttcgatgcac
    16381 cgctgcagat gaaattccat gaagcatcgc gcatggggcg cgactacgac atgacgcaga
    16441 ttttcacggg tacattagtg gaagccgatc ctttccacgc cgtgacgctc gttgccaatc
    16501 acgacaccca accgttgcaa gccctcgaag cgccggtcga accgtggttt aaaccgctgg
    16561 cgtatgcctt aattttgttg cgggaaaatg gcgttccttc ggtgttctat ccggacctct
    16621 acggtgcgca ttacgaagat gtcggtggtg acgggcaaac ctatccgata gatatgccaa
    16681 taatcgaaca gcttgatgag ttaattctcg cccgtcagcg tttcgcccac ggtgtacaga
    16741 cgttattttt cgaccatccg aactgcattg cctttagccg cagtggcacc gacgaatatc
    16801 ccggctgcgt ggtggtcatg tcgaacggag atgatggcga aaaaaccatt catctgggag
    16861 agaattacgg caataaaacc tggcgtgatt ttctggggaa ccggcaagag agcgtagtga
    16921 cagacgaaaa cggcgaagcg accttctttt gcaacggcgg cagcgtcagc gtgtgggtta
    16981 tcgaagaggt gatttaaatc catccccggc ggcaagccgg ggagatttca ttacggcagt
    17041 ggcgtcggta acggcgcttt atccagcgcg gcggcacact ctgccgtcgg gcgatccaca
    17101 cgctccatcg tcataccatc gtactcaatg gtgttaccgt cacgctcaac ctcgtacagc
    17161 tcacgtttta cggtaacgtt agtgagatca tcagacatca atgtcaactt acccggcacg
    17221 gcaatcaccc gctgccactg acggcaatcc agggtatcgc cctctttagt gacaatgagg
    17281 ctgcctatcg cttccggact gaccaacgca cgttgcggcc ctttggtctg ccagtaaccc
    17341 gtcagccagt ccggcgcggg ggttttcacg acattgttat agttttcgac ttcggcacag
    17401 cccgccagca acatgagcgc acctgcaatt gcgagttttt tcatcattcg tcctgcatga
    17461 gaagaaaaga ggattgtggc attaaagccc tgatgtcgcc agcctttcca ggctcagaga
    17521 gaaatttgat ctaaccatta tttttgtgaa ttattacccc gaaaataaat ttgcatggtg
    17581 tatgattcgc gactcttgtt tcaaaccttc tgagttcaga ggctacattc atgtcatggc
    17641 agcaattcaa acacgcctgg ttgattaaat tctgggcacc catccctgcg gtcatcgcgg
    17701 cgggtattct ctccacttac tattttggca tcactggcac cttttgggca gtcacgggtg
    17761 aatttacccg ttggggcggt cagcttttgc agctgtttgg cgtccatgct gaagagtggg
    17821 gttattttaa aattatccat ctggaaggat cgccattaac ccgcatcgac ggaatgatga
    17881 tcctcggtat gtttggcggc tgttttgccg cagcgctgtg ggccaacaat gtcaaactgc
    17941 gcatgccgcg cagccgtatc cgcattatgc aggccatcat tggcggtatt atcgccggtt
    18001 ttggcgcacg tctggcaatg ggctgtaatc tggcggcgtt ctttaccgga attccacagt
    18061 tctcgctgca cgcctggttc tttgccatcg ccactgccat tggttcatgg tttggcgcgc
    18121 gctttaccct gctgcccatc ttccgtattc ccgtgaaaat gcagaaagtt tctgccgcct
    18181 caccgctgac gcaaaaaccg gatcaggcgc ggcgtcgttt tcgtctcggg atgctggtct
    18241 ttttcggcat gctgggctgg gcgctgctga cggcgatgaa ccagcccaaa ctggggctgg
    18301 caatgctgtt tggcgtcggc tttggtttgc tgattgaacg tgcgcaaatc tgctttactt
    18361 cggcgttccg cgatatgtgg atcaccggac gtacccatat ggcgaaagca atcattatcg
    18421 gtatggcggt aagtgccatc gggatcttca gttacgtaca gttaggcgtt gaacccaaaa
    18481 tcatgtgggc gggaccaaac gcggtaattg gtggtttact gtttggtttt ggcatcgtgc
    18541 tggcaggcgg ctgcgaaacc ggctggatgt accgcgcggt agaaggccag gtgcactact
    18601 ggtgggtcgg tctgggcaac gtgatcggct caacgattct ggcgtattac tgggatgatt
    18661 tcgctccggc gctggccacc gactgggaca aaatcaacct gctgaaaacc tttggcccga
    18721 tgggcggcct gctggtgaca tatttgctgt tgtttgcagc tctaatgttg attatcggct
    18781 gggaaaaacg cttcttccgc cgtgcggcac cgcagattgc taaggagatc gcatgaaaaa
    18841 tatcgttcct gattaccgtc ttgatatggt gggtgaaccc tgcccttatc cggctgtcgc
    18901 aacccttgag gcgatgccgc agttaaaaaa aggggagatc ctggaagtgg tgagcgactg
    18961 tccgcagtcg atcaataata ttccactgga tgcgcgtaat cacggctata cggtgctgga
    19021 tattcagcaa gacgggccga ccattcgtta tttaattcag aagtaatccc tcattcccgc
    19081 tgtggcccac agcgggaaca tcgttacctc tctatcacat agattttacc cgccccctct
    19141 cctattcttt tcagtgtgct ctcaggaggt ttctatgtgt ggacgctttg cccaatccca
    19201 aacgcgtgaa gattacctcg cgtttctcgc ggaagatatt gaacgcgata ttccctacga
    19261 tcccgaacct attggcagat acaacgtcgc gccgggaact aaagtccttc tgctcagtga
    19321 acgtgatgaa caccttcatc tggatccggt tttctgggga tatgctcccg gatggtggga
    19381 taaaccgccg ctgattaacg cccgcgtaga aactgccgca accagtcgca tgtttaaacc
    19441 gctctggcaa catggtcggg taatctgctt tgccgatggc tggtttgagt ggaaaaaaga
    19501 aggcgacaaa aacagcctta ttttatctat cgcgctgatg gacaacctgt ttttatagcc
    19561 gcgataggta gcacaccgtt tgagcgtggt gacgaagccg aaggattttt aattgtcact
    19621 gcagcagcag accagggtct ggtggacatt catgaccgcc gcccacgagt actgtcgcca
    19681 gaaactgcgc gggaatggat gcggcaagac attggtggta aagaagcctc agaaatcgct
    19741 accagaagct gtgtgccagc aaaccagttc acctggcacc ctgtgtcgcg cgcggtgggt
    19801 aatgttaaaa accagggcgc ggagttaatt caacctgttt gataagcctg gagattatct
    19861 acacctggat ggacatcatt gtactatgcc ccgtacatgc cccatcacat cctcaacatc
    19921 aaagtattag agccttgcaa accattatgc gtctcagttt gtctcatcta tcttacgcaa
    19981 aaaaaaatag aactaatatc aagcggcgct atattgcgta tt
//
LOCUS       DAZFFE010000087        19847 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZFFE010000087 DAZFFE010000000
VERSION     DAZFFE010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.087"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            189..692
                     /gene="tssB"
                     /locus_tag="ACHJ69_003426"
     CDS             189..692
                     /gene="tssB"
                     /locus_tag="ACHJ69_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO5016342.1"
                     /translation="MSKMNNNGGSVAPKERISVRYTPKVDGVAADIELPLNLLITGNL
                     KGKPDNTPLDERTAIAINRYNLNAVISEADIEREFVVPAELSDAPNEQMYVNLKVKSM
                     DDLSPDHIASQVPEIKRLLELREALVALKSPLGNIPAFRAQLQALLENEDTREQLIQE
                     PGIAVQK"
     gene            715..2199
                     /gene="tssC"
                     /locus_tag="ACHJ69_003427"
     CDS             715..2199
                     /gene="tssC"
                     /locus_tag="ACHJ69_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO5016343.1"
                     /translation="MSVKEEIAPVSASQTAAPPSLLDEIMAQTRVQPKSESYDITRQG
                     VSAFIAAMLQGDSSAEPINILAVDAMIADIDARTSRQMDAIIHAPEFQELESLLRSLK
                     LLVERADTRENIKVHFLNVTQEELLDDFEFAPEITQSAYYKHVYSSGYGQFGGEPVAA
                     VIGNFAFKNTTPDMKLLKYISQVSAMAHSPFLSSVSSEFFGLDSWTELPGIKEPGAIF
                     EGPAYSRWRALRESEDSRYLGLTAPRFLLRHPYSPDENPVKTFRYHEDVSQSHESYLW
                     GNTSFLLAANLAESFAKYRWCPNIIGPSSGGAVKDLPVHLYESMGQMQAKIPTEVLIT
                     DRREYELAEEGFITLTMRKGSDNACFFSANSVQKPKTFPKTPEGKAAETNYKLGTQLP
                     YLFVISRLAHYIKVIQREQLGSWKERSDLERELNTWIRQYVADQENPPAEVRSHRPLR
                     QAKIEVLDVDGEPGWYQVAISVRPHFKYMGASFDLSLVGRLDKE"
     gene            2204..2629
                     /gene="tssE"
                     /locus_tag="ACHJ69_003428"
     CDS             2204..2629
                     /gene="tssE"
                     /locus_tag="ACHJ69_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO5016344.1"
                     /translation="MLRRDSGPTGSLFERIREAANPPSYQNPKEALIRSIRRNLRQVL
                     NTRSGSCYGSPELGITDLNDESLASSDFRREIRKSISQCILHYEPRITDVVVTAAAPD
                     EYAPVELRFHIVATIDVSETRGVFEFDILLDNHQRYCVE"
     gene            2635..4476
                     /gene="tssF"
                     /locus_tag="ACHJ69_003429"
     CDS             2635..4476
                     /gene="tssF"
                     /locus_tag="ACHJ69_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO5016345.1"
                     /translation="MAFEERYYREELDYLRQLGKLLAQEKPHLAHFLAEKEGDPDVER
                     LMEAFAFMSGSLRQKLEDEFPEFTHGLIRMLWANYLRPVPAMTVIAYEPKTDQLKVPV
                     QVCRNELIRSRSENSSLTAQKVLPDNHDKIVSSVACHFTLARDIWLQPLRILDTRNAS
                     SLKEGIIDISFTADNNVSPAMLDLNKITFWLGNEDDYTRHQLYLWFCECLMDAELIAG
                     EYRLPLPDLWLDAAGFDNRDALLPWPKNVHSGYRVLQEYFCYPEAFFFFHLRDVAPLP
                     DDFPVNAFTLRLHFNRPLPADIKLRQDSLRLHCTPAINLFTHYAEPVRPDGRMAEYPL
                     RASHKHPDAYDIFQVSTVTSKVKVSGTEISPGSQARVWPEFESFQHQMEYSRQREVVY
                     WHHRTKTSLFHYGLEHSIAFVHADGSIPDSSRFNDDVITTSLICTNRMIPARLHTGDI
                     CVAVNKNPAVASFRNVTRPTLPLWPVTDGDMHWSLISAMNLNYLSLLDRETLIQILRT
                     FDLPGAHHPQRARLSRQKLDAIEKLETKPVDRLFKGVPVRGLATTLWIRPDPFICEGE
                     IYLLGTVLSHFFALYASINSYHCLKIINTESQESWEWQEKMGQHALI"
     gene            4440..5489
                     /gene="tssG"
                     /locus_tag="ACHJ69_003430"
     CDS             4440..5489
                     /gene="tssG"
                     /locus_tag="ACHJ69_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO5016346.1"
                     /translation="MAGENGPARPDIATKPPLPADVRNCNFYVLMEALYRRHGAPGQD
                     ISLRTEPAREIVRFSSDASISFPGTDLSALSRSQNGQYVLQTRFLGFSGSQSPLPGYY
                     LDQMAQESAQNEDGLKEFRDLFSHRWTQFAYHAWRKYRYYICFRSGGTDTFSQRMYAL
                     VGLGNQSVRDRLAINHSKMLAYAGILATPGRAPEVICNLVSHCFDLPDVTIERWQLRK
                     VAIDPAHQNRLGVRNPKGKTAGHIPGRSILGVNFTLGARVPDRSGKFLLQIGGLSRER
                     YLSFLPDGENHLPLTMFLSFILRDQFAWDLRLCLAPQQAKGMRLGDPASSRLGRTSFI
                     GQPKVPPAITIRIRE"
     gene            5506..6786
                     /gene="tagH"
                     /locus_tag="ACHJ69_003431"
                     /pseudo
     CDS             5506..6786
                     /gene="tagH"
                     /locus_tag="ACHJ69_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001511117.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
     gene            6783..7307
                     /gene="tssJ"
                     /locus_tag="ACHJ69_003432"
     CDS             6783..7307
                     /gene="tssJ"
                     /locus_tag="ACHJ69_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO5016347.1"
                     /translation="MNGKAFLACVLMSVVLTGCETAKKISQVIRNPDIQVGKLMEQST
                     ELTVTLLTEPDSNLTADGEAAPVDVQLVYLSDDSKFQAADYDQIATTALPDVLGKNYI
                     DHQDFNLLPDTVKTLPPIKLDEKTGYIGVIAYFSDDQATEWKQIEPVESIGHHYRLLV
                     HIRASAIEMKKEEN"
     gene            7310..8656
                     /gene="tssK"
                     /locus_tag="ACHJ69_003433"
     CDS             7310..8656
                     /gene="tssK"
                     /locus_tag="ACHJ69_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO5016348.1"
                     /translation="MATMKNKVIWQEGLFALPQHFQQQQRHCEYLLTRRLDAQGDFFW
                     GFTELSINTELLAQGKIMIDRAAGCMPDGTVFSIPDQDLLPEPFQPGTLSSKESHNIY
                     LALPVISDVINEIQGLHSAGQGTERYRLTHTRVRDFHTDEGDEQPVGLGQLIPRIVSG
                     ADDLSAMVTLPLCRILNKNATGALVLDNTFIPTIQAVHVSGLLGAFSGEVQGLLATRA
                     ADLAGRIGSPEQSGIADVAEFMMLQMLNRYQMQFTHRSQLHTLHPEAFYRDLVGLLGE
                     LMTFTEGNRLPCTVEPYNHRDLTATFTRTVIPELRRALNTVLVPRAQNLPLFFSEGTW
                     IATINDPTLLQSCKLVLAVRARMPYDQVQRQFIQQSKVAATDKIRSVVSVQVPGIPLH
                     TLTAAPRQLPYHEGYVYFELEKGTPAWQDVIKAGALALHISGSFPDLNLQLWAIRG"
     gene            8661..9425
                     /gene="icmH"
                     /locus_tag="ACHJ69_003434"
     CDS             8661..9425
                     /gene="icmH"
                     /locus_tag="ACHJ69_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO5016349.1"
                     /translation="MSDMSEASVFPVEPESGTPGRQYRLALRGNSLNPMIDAATPLLG
                     MVMRLSTMNSQAMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIVSFRYILCTFIDEAAL
                     GNGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPVRYQDLLEFLYLCFSLGF
                     RGRYKVAIQQQDEFERIYQRLHHALHKLRGDAPFPLLHQKNNIQGGRYQLIRRLTIKH
                     VLCGGLVVLTVFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9435..12107
                     /gene="tssH"
                     /locus_tag="ACHJ69_003435"
                     /gene_synonym="clpV"
     CDS             9435..12107
                     /gene="tssH"
                     /locus_tag="ACHJ69_003435"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO5016350.1"
                     /translation="MIQIDLATLVKRLNPFAKQALEMAASECMSQQASEITVAHVLLQ
                     MLAIPRNDVRVIAERTGISAEDLRQALTVESYPGGRSAEGYPSFSPMLIEWLKESWLL
                     ASAQMQHSELRSGVLLLTLLHSPLRYIPPAAARLLTAINRDQLQQDFAAWTKESAESV
                     NQVGGQAPGTPETGDSGLARYAKNMTEDARNGKLDPVLCRDHEIDLMIDILCRRRKNN
                     PVVVGEAGVGKSALIEGLALRIVSGQVPDKLKNTDIMTLDLGALQAGASVKGEFEKRF
                     KGLMAEVISSPVPVILFIDEAHTLIGAGNQAGGLDVSGLLKPALARGELKTIAATTWS
                     EYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAAAT
                     LSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLEREL
                     RIGLRTDTSRMTEVLEQYDETLSALDELEVAWQQQQQTLVQEIIALRQQLLGAAEDTD
                     AVVEEVTETVSPVQRLAQLTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLS
                     QNEMSVITDLPKWLGDTIKGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVG
                     KTETVLQLAELLYGGRQYLTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAVRQ
                     KPYSVVLLDEVEKAHPDVLNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIV
                     EHADDPETMQEALYPVLADFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLR
                     SRFGADVIIGPEVTDEIMSRVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIA
                     RIRLSAADGAFTADVEDALPDDAVTPQTEDETVL"
     gene            12104..12847
                     /gene="vasI"
                     /locus_tag="ACHJ69_003436"
     CDS             12104..12847
                     /gene="vasI"
                     /locus_tag="ACHJ69_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO5016351.1"
                     /translation="MSRLCVFRQTGWFIAGLMAGLPASAAPTETSSVAGVAVAVATTT
                     PPDATATLQAMQSCRQETAALERLDCYDRILAPEQAGFGGAALVKARYQGEAWARATE
                     QEKRRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGTD
                     NAAGKLTFNIDGLARVIAPLRDACHWAGE"
     gene            12852..14294
                     /gene="tssA"
                     /locus_tag="ACHJ69_003437"
     CDS             12852..14294
                     /gene="tssA"
                     /locus_tag="ACHJ69_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO5016352.1"
                     /translation="MAMDLRNPDVWLAHLLENLPEDKLSAALDDGNADWEFVDSEIVK
                     LGSLAHSQLDIPELQRRGLMLLASETKDFRLLAHLLRTLQHAGDILLASRMLAQYTEH
                     YWTCAAPQNMAHKKRFAAQVIKRFESAVQDFAGNAATIQRDALLGELAKLAQCWQAHN
                     ASELAKATDDLFSLFQRAFRDVAPEMTSSARSAAIAPQATTGYVPDMSPHTVAAQPPV
                     AATPIPQVTVESHDDKAWRDTLLKVAAILCERQPESPQGYRLRRHALWQTITSTPQAE
                     SDGRTPLAAFPVDMMDDYLARLNNADMALWQQVEKSLLLAPYWLDGHYLSAQTALRLG
                     YKQVADAIRDEVTDFLARLPALINLLFNDRTPFVSEQTKQWLASSGSVNQTVPVVQTD
                     EELQAAKACFDENGLEAALRYLENLPEGDPRHQFHRQFFGAQLLEEAGMVQLAQQQYR
                     MLFRTGLHMMLSEWEPSLLKALEQKLTAEQ"
     gene            14374..17838
                     /gene="tssM"
                     /locus_tag="ACHJ69_003438"
     CDS             14374..17838
                     /gene="tssM"
                     /locus_tag="ACHJ69_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO5016353.1"
                     /translation="MPRFKVSATWLLTLAWIFLLVWIWWQGPKWTLYEQHWLAPLANR
                     WLATAVWGLIALVWLTWRVMKRLQKLEKQQKQQREEEKDPLTVELHRQQQYLDHWLLR
                     LRRHLDNRRYLWQLPWYMVIGPAGSGKSTLLREGFPSDIVYTPESIRGVEYHPLITPR
                     VGNQAVIFDVDGVLTTPGGDDLLRRLREHWLGWLMQTRARQPLNGLILTLDLPDLLTA
                     DKSRRETLVQNLRQQLQEIRQSLHCRLPVYVVLTRLDLLNGFAALFHSLDKKDRDAIL
                     GVTFTRRAHESDGWRSELGAFWQTWVQQVNLALSDLVLAQTGAAPRSAVFSFSRQMQG
                     TGEIVTALLAALLDGENMDVMLRGVWLTSSLQRGQVDDIFTQSAARQYGLGNSSLATW
                     PLVETTPYFTRRLFPEVLLAEPNLAGENSVWLNSSRRRLTAFSTCGAALAALMVGSWH
                     HYYNQNWQSGVNVLAQAKAFMDVPPPQGTDEFGNLQLPLLNPVRDATLAYGDYRDHGF
                     LADMGLYQGARVGPYVEQTYIQLLEQRYLPSLMNGLIRDLNIAPPESEEKLAVLRVVR
                     MMEDKSGRNNEAVKQYMARRWSNEFHGQRDIQAQLMVHLDYALEHTDWHAQRQSSDSD
                     AVSRWTPYNKPVINAQHELSKLPIYQRVYQTLRTKALSVLPADLNLRDQVGPTFDNVF
                     VAGNDEKLVIPQFLTRYGLQSYFIKQHDGLVELTALDSWVLNLTQSVAYSEADREEIQ
                     RHITEQYLSDYTATWRAGMDNLNVRDYETMPALTDALEQIISGDQPFLRALTALHDNT
                     HALTLSGKLDDKAKEAAINEMDYRLLSRLGHEFAPENSALEEQKDKASTLQAVYQQLT
                     ELHRYLLAIQNSPVSGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGKLAD
                     QAWHVVMVEAVRYMEVDWRDNVVKPFNEQLADNYPFNPRATQDASLDSFERFFKPDGI
                     LDNFYKNNLRLFLENDLTFGDDGRMLIREDIRQQLDTAQKIRNIFFSQQNGLGAQFAV
                     ETVSLSGNKRRSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESQLTLIGTSGRAPR
                     SIAFSGPWAQFRLFGAGQLTNVTSDTFNVRFNVDGGAMVYRVHVDTEDNPFTGGLFSQ
                     FRLPDTLY"
     gene            17849..19207
                     /locus_tag="ACHJ69_003439"
     CDS             17849..19207
                     /locus_tag="ACHJ69_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO5016354.1"
                     /translation="MSNNALTQTIVTGSDPRALPEFSAIREEINKANHPSQPELNWKL
                     VESLALSIFKANGVDLHTATYYTLARTRNQGLAGFCEGAELLAAMIIHEWDKFWPQSG
                     PARTEMLDWFNTRTGNILRQQVSFSENDLSLLYRTERALQLICDKLQQVELKRQPRVE
                     NLLYFVQNTRKRFEPQPRNRTDTAAQTMVRTLVYAPEGTASATAETMPPLPDLPEMRV
                     GVRGVADNADKAKQGDTIKGFVAGAACTAVIASALWWWQAYPMQQQLTQVRDTTQGAA
                     TVWLASPVLKEYEQYLQQLLNAPPLQPLETGMQMMRTADTLWPESLQQQEASRMWSNT
                     LRNRAQASPQMKGWQQARQNLRDFADLMMKKETEKQGFTLSYIKTVTWQAERLLNQET
                     PLEYLLTQYQETRTQKQDTQALEKEINERLDGLLSRWLLLKNTGQDMATDNRTEPVHP
                     TH"
     gene            19225..19704
                     /locus_tag="ACHJ69_003440"
     CDS             19225..19704
                     /locus_tag="ACHJ69_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO5016355.1"
                     /translation="MANPVYLTLNGELQGLISSGCSSMPSIGNKAQIAHQDQIMVTSL
                     SHGLSRAQNVNHQELTITKPVDKSSPLLGKAISENECLTCDFVFYRTNRFGINEPYYK
                     LKLANARLSNIGLTVPHTINDSPGQAEESVSFTYESINWEHSVAGTSAYSLWSERIF"
BASE COUNT         4637 a         5548 c         5494 g         4168 t
ORIGIN      
        1 tgtattaata atgaaggatg catctctttc aaaaatataa atagttcttt taatatttta
       61 tttcatgagt agaataccga tcacaacaga aggaaggtta cattatctaa ctgccactga
      121 aacggtattg tcggtatttg tatgcagtac gaaaatgctg tgctcatggc ctgaacggga
      181 acatttttat gagcaaaatg aacaacaatg gcggcagtgt cgcgccgaaa gaaagaatca
      241 gcgttcggta tacaccaaaa gttgatgggg tggctgctga tatcgaattg ccgctgaatt
      301 tattgatcac cggcaatctg aaagggaaac cagataacac gccgttggat gagcgaactg
      361 ccattgctat caaccgctat aacctcaacg cagtcatttc agaagctgac attgaacggg
      421 agtttgttgt tccggctgaa ctcagtgacg ctcccaatga gcaaatgtac gtcaacctga
      481 aagtcaaatc tatggatgac ctttctcctg accatattgc cagccaggta ccagaaataa
      541 aacgtctgct cgaattgcgt gaagccctgg tcgcacttaa gtctccacta ggcaatattc
      601 cagccttccg cgcacaactt caggcgctac tggaaaacga agacacccgc gagcagctga
      661 tccaggaacc gggtatcgcc gttcagaaat aattttcaca gaaggatatt cagtatgtca
      721 gtaaaggaag aaattgctcc cgtcagcgcg tctcaaaccg ccgcgccccc atctctgctt
      781 gatgaaatca tggcgcaaac ccgcgtccag cctaaatcag aaagctacga tatcacccgc
      841 cagggcgtga gcgcctttat tgccgccatg ttacagggcg acagtagcgc tgaacctatc
      901 aatatcctgg ccgttgacgc catgattgct gatattgatg cccgaaccag caggcagatg
      961 gatgccatta ttcatgcccc ggagtttcag gaactggaat ccctgctgcg ctcgctgaag
     1021 ctgctggttg aacgcgcgga tacacgggaa aacatcaaag tccatttcct gaatgtcact
     1081 caggaagaac tgctggatga tttcgaattt gcacctgaaa tcacgcagtc tgcgtattac
     1141 aagcatgtat attcaagcgg ttacggacaa tttggcggtg agccagtggc ggcggtaatc
     1201 ggcaactttg cctttaagaa caccacgccg gatatgaagc tgctgaaata tatcagccag
     1261 gtcagcgcga tggcgcacag cccgttcctg tcgtcagtat catccgaatt tttcggtctg
     1321 gattcatgga cagagttacc ggggatcaaa gaaccgggtg ccatctttga aggcccggcc
     1381 tactcccgct ggcgcgccct gcgtgaatcg gaagattccc gttacctggg cctgaccgcc
     1441 ccccggttcc tgctgcgcca cccttactca ccggatgaaa accctgtcaa aaccttccgt
     1501 taccatgaag atgtcagcca gagtcatgag tcttatctgt gggggaatac ctcgttttta
     1561 ctggccgcca acctggcgga aagttttgct aagtatcgct ggtgcccaaa tattattggc
     1621 ccttccagcg gcggcgcagt caaagacctc ccggtgcatc tgtatgaatc catggggcaa
     1681 atgcaggcaa aaattccgac tgaagtgttg atcactgacc ggagagagta tgagctggcg
     1741 gaagagggct ttatcacact gaccatgcgt aaagggtctg ataatgcgtg ctttttctcg
     1801 gccaactcgg tgcagaaacc caaaacattc cccaaaaccc ccgaaggcaa ggctgcggag
     1861 accaactata aactcggcac ccagctgcca tacttgtttg ttatcagcag gttagcgcat
     1921 tacatcaagg taattcagcg cgaacagctt ggctcatgga aagaacgtag tgacctggag
     1981 cgtgagctga acacctggat tcgccagtat gttgctgacc aggaaaatcc gcctgccgaa
     2041 gtccgcagcc accggccttt gcgtcaggct aagatcgagg tactggatgt ggatggtgag
     2101 ccgggctggt accaggtcgc gatttctgtc agaccgcatt ttaaatacat gggtgccagt
     2161 ttcgacctga gcctggtcgg acgactggat aaggaataat ccgatgctaa gacgggattc
     2221 aggaccaacc ggcagtcttt ttgaacgtat ccgtgaggcg gcaaatccgc cttcatatca
     2281 gaatccaaaa gaagcactga tacgttccat cagacgtaac ctgcgacagg tcctaaatac
     2341 ccgctccggc agttgttatg gctcaccgga gctggggatt actgatttaa atgatgaatc
     2401 gctggcctcc agcgatttca gacgagaaat ccgtaagtcc attagccagt gcattttaca
     2461 ttacgaacct cgtattaccg atgttgttgt tacggctgcg gcaccggatg aatacgcccc
     2521 tgtggaactg cgttttcata ttgtggctac gatcgatgtc agcgagacca ggggagtgtt
     2581 tgaatttgac attttgctgg acaaccatca acgctattgt gtggagtgat ctctgtggct
     2641 tttgaagaac gttactaccg cgaagagttg gattatctgc gccagcttgg caaactgttg
     2701 gcgcaggaaa aaccgcatct ggcccatttt ctggccgaga aagagggtga tccggatgtg
     2761 gagcgactga tggaagcctt tgcctttatg tccggtagcc tgcgccagaa acttgaagac
     2821 gaatttcccg aatttaccca tgggctgatc cgtatgctgt gggcaaacta tttacgcccg
     2881 gttccggcca tgacggttat tgcctatgaa cccaaaaccg atcagttaaa agtccctgtt
     2941 caggtctgtc gtaatgaact gatcagaagc cgctcagaaa actcctccct gactgctcag
     3001 aaggtgctgc ctgacaatca tgataaaatc gtttcttccg tagcctgcca tttcacgctg
     3061 gcgcgcgata tctggctgca accactgcgc attctcgata cccgcaacgc cagcagcctg
     3121 aaagaaggca tcatagatat cagttttacc gcagataaca atgtctcccc cgccatgctt
     3181 gacctgaaca aaatcacgtt ctggctgggt aatgaagacg attatacccg acaccagctt
     3241 tatctgtggt tttgcgaatg cctgatggat gcggaactga ttgccggaga gtaccgcctg
     3301 ccgctgccgg atctgtggct ggatgctgcg ggttttgaca accgggatgc tctgctgccg
     3361 tggccgaaaa atgtccacag cggctaccgc gtgcttcagg agtatttctg ctatcccgaa
     3421 gccttcttct ttttccatct gcgcgatgtt gcgccgctac cagacgattt cccggtcaat
     3481 gcgtttacgc tgcgcctgca ctttaaccgc ccgttacctg ccgacatcaa gctgcgccag
     3541 gattcgctgc gtctgcactg tacgccagcc attaacctgt ttacacatta tgcggaaccc
     3601 gtcaggccgg atgggcggat ggcggaatac ccgctgcgcg ccagccataa gcatccggac
     3661 gcctatgaca tttttcaggt cagcacagtc accagtaaag tcaaagtttc aggtactgaa
     3721 atcagcccag gtagccaggc gcgtgtctgg cccgaatttg aaagcttcca gcaccagatg
     3781 gagtacagcc gccagcggga agtcgtctac tggcatcacc ggactaaaac gtccctgttt
     3841 cactacgggc ttgaacatag tatcgccttt gtccatgcag acggtagcat tccggacagc
     3901 tcacggttta acgatgacgt gatcaccacg tcattaatct gtaccaaccg tatgatccct
     3961 gcccggctgc ataccggtga tatctgcgtg gccgtcaaca aaaatccggc agtggcctca
     4021 tttcgtaatg tcacgcgccc gacactgccg ctctggccag tcacggacgg cgacatgcac
     4081 tggtcgctga tttccgcgat gaacctgaac tacctctccc tgctggacag ggagacactg
     4141 atccagatcc tgcggacctt cgacctgcca ggtgctcacc atccgcagcg ggccagactg
     4201 tcacgccaga aactggatgc catagaaaaa ctcgaaacca aacctgttga caggttgttt
     4261 aaaggcgtac cggttcgcgg gctggcgacc acgctgtgga tacgtcccga tccgtttatc
     4321 tgcgaaggtg aaatttatct tctgggtacc gtgctctcgc acttctttgc cctctacgcc
     4381 agcatcaatt cgtaccactg tctgaaaatc attaacacgg aaagtcagga gtcctgggaa
     4441 tggcaggaga aaatgggcca gcacgccctg atatagcgac aaaaccgccg ttaccggcgg
     4501 atgtgcgcaa ctgcaatttc tatgtgctga tggaggcgct gtaccgtcgg catggcgcgc
     4561 cgggccagga catttccctg cgtaccgagc ctgcccggga gatcgtgcgt ttcagctctg
     4621 acgccagtat cagttttccg ggtacggacc tgagtgcgct ttcccgtagc cagaacggac
     4681 agtatgtact gcaaacccgg ttcctgggtt tttccggcag ccagtcgccg ctaccgggct
     4741 attatctgga tcagatggcg caggagtccg cacagaacga agacggcctg aaagaatttc
     4801 gcgatctatt cagccaccgc tggacacagt ttgcctatca tgcctggcgc aaataccgct
     4861 actacatctg ctttcgtagc ggcggtactg acaccttttc acagcgcatg tacgcactgg
     4921 tcgggctggg taatcagagc gtgcgtgaca ggcttgccat taaccacagc aaaatgctgg
     4981 cctacgccgg aattctggca acaccaggcc gcgcgccgga ggttatctgt aatctggtgt
     5041 cgcactgttt tgatttgccg gatgtcacca ttgaacgctg gcaactgcgt aaagtcgcca
     5101 ttgatccggc acaccagaac cggctgggtg tacgtaatcc gaaaggaaaa acggctggac
     5161 atattccggg acgctccata ctcggcgtta attttaccct cggcgcacgg gtgccggatc
     5221 gcagcggaaa attcctgttg cagattggcg gcctgtcgcg ggagcgttat ctctcatttt
     5281 tacccgatgg cgaaaatcac ctgccgctga ccatgttcct ttcctttatt ctgcgcgacc
     5341 agtttgcctg ggatttacgg ctgtgcctgg cgccacagca ggctaaaggg atgcgccttg
     5401 gcgatccggc cagctcccgc ctcggcagaa ccagctttat tggtcagccg aaagtaccgc
     5461 ccgccatcac aatccgaatc agggaataat tctatggaac cacttatggc tgaggaaaaa
     5521 cagcaatccc gccaggcctc actgacttta caggtgatga atggcaacga actggaaagc
     5581 ggacgcgccg caaaatgcct gttcagcgaa aacggcggtg atattggcca tacgccggaa
     5641 tgtcactggc aggtacagga ccgggcggga agcattgctg cgcgtgcctg taccgttatc
     5701 cggcacgacg gagcctattg cctgcggtgc ctgacgccgg gactgatgat taatctggcc
     5761 ccggcctctt ccgatgcgct catccgcctg cgccaggggg atgagatcca gctcggcgca
     5821 ctggcgctga aagtgtttct gcatgacggt gcagctgtta cctatgacga gcggatggcg
     5881 accccggaaa cgattgtaat gaaccgcgac agtctggcgg acaccctgct gaccaccgag
     5941 ggacaaccgg cgtatccggg aatgccgttc cagcatcagc ttgcccccac ggtggcgcac
     6001 ggtttttcca gcgatccgct ccaggcactc cagacggaaa gcctgatggt ggcagatgat
     6061 ccgatcgccc cacgcgtttc ccggcccgta gcccccgcat ccgatctgac aggtgcaaac
     6121 gggatcaaca cgccgtttat ggatctgccg cccggcaatg cccgtcatga ggacggcgat
     6181 gaattttctg ccatggcgca gtaccacctg gccgtaaccc cgctgctgcg cggtatggag
     6241 tgtccgctga cgctgcacaa ttctcaggat gcggatgagt ttctcgaaga ggccggacgg
     6301 gcgttgcagg ccgccattaa gggattgctg agtctgcaac aacaacagaa tagcctgtcg
     6361 gataaacacc tgcgtccact ggaagacaac ccgttgcgcc ttgatatgga ctatgccacc
     6421 gcgctgaacg tcatgttcgc cgagggtaaa agcccggtac atctcgcagc acctgcggcc
     6481 atcgccgaaa gcctgcgcaa catccggcat catgaagagg ccaaccgggc ggcgattgtg
     6541 gaagcgttgc gtgtcatgct ggacgccttc tcgccgggca acctgatgcg ccgctttgcg
     6601 taataccgcc gcagccatga gctgcgccag aaaatggaca acgcctgggc atggcagatg
     6661 tacagcaact attacgatga actggcctcc agccgacagc agggatttga aatgctgttc
     6721 aatgaagttt atgcccaggt ctatgaccgg gtgctgcgcg aaaaacagcg ggagccggaa
     6781 gcatgaacgg caaagcgttt ctggcctgcg ttctgatgag cgttgtgtta actggctgtg
     6841 aaacagcgaa aaaaatcagc caggtgatcc gcaatccgga tattcaggtc ggaaagctga
     6901 tggagcagtc aaccgaactg accgtcacgc tgctgaccga gccggacagc aacctgacgg
     6961 cggacggcga agccgcgccg gtggatgtcc agttggtgta tctgagcgac gactccaaat
     7021 tccaggccgc cgattacgat cagattgcca ccaccgcgct gcccgacgtg ctggggaaga
     7081 actatatcga tcaccaggac ttcaacctgt tgccggacac cgtaaaaaca ctgccgccga
     7141 tcaagctgga tgagaaaacc ggttatatcg gtgtcattgc ctatttttca gacgaccagg
     7201 ccacagaatg gaaacaaatt gagccggtag aaagtatcgg ccaccactat cgcctgctgg
     7261 tgcatatccg cgccagtgcg attgagatga aaaaagagga aaactgacga tggcaacaat
     7321 gaaaaacaag gtgatctggc aggaagggct gtttgccctg ccgcagcatt ttcagcaaca
     7381 gcagcgtcac tgcgagtacc tgctgacccg tcgtctggat gctcagggtg attttttctg
     7441 gggctttacg gaactctcca tcaataccga actgcttgcc cagggcaaaa tcatgattga
     7501 ccgtgcggcg ggctgtatgc ctgacggcac cgtattcagc attcccgacc aggatctgct
     7561 gccggaacct ttccagccgg gaaccctttc atcgaaagag agccacaata tttatctggc
     7621 gctgcctgta atcagcgacg tgatcaatga aatccagggg ttgcacagcg ccggtcaggg
     7681 tacggagcgt taccgcctca cccatacccg cgtccgtgat tttcacactg acgaaggcga
     7741 cgaacagccg gtcggtctgg ggcagttgat ccccaggata gtcagcggcg cagatgatct
     7801 cagcgcgatg gtgaccctcc ccctgtgccg tatcctcaat aaaaatgcta ccggcgcact
     7861 ggtgctggac aacaccttta ttcccaccat tcaggccgtc catgtcagcg ggttgctcgg
     7921 ggcattctcc ggcgaggtgc agggactgct tgccacccgc gccgctgacc tggccgggcg
     7981 cattggttcg cctgaacaga gcgggatcgc cgatgtggcc gaatttatga tgttgcagat
     8041 gcttaaccgc tatcaaatgc agttcacgca tcgttcccaa ctgcacacgc tgcatccgga
     8101 agccttctac cgggatctgg tggggctgct gggagaactg atgaccttta ccgaaggtaa
     8161 tcgcctgccc tgcaccgtgg agccgtataa tcaccgggat cttaccgcga ccttcaccag
     8221 aaccgtgatc ccggaactgc ggcgcgcgct caataccgta ctggtgccgc gtgcgcagaa
     8281 tctgccgttg ttcttttcag aggggacctg gatcgccacc atcaacgatc ccacattgtt
     8341 gcagtcctgc aagctggtac tggcagtgcg cgccaggatg ccctacgacc aggtacaacg
     8401 ccagttcatc cagcagtcga aagtggccgc taccgacaaa atccgcagcg tggtcagcgt
     8461 gcaggtgccg ggtattccgc tgcacacgct taccgctgcg ccacgtcagt tgccatacca
     8521 cgaaggttat gtttacttcg aactggaaaa aggcaccccg gcctggcagg acgtgataaa
     8581 agcgggcgca ctggcgctgc atatctccgg cagcttcccc gatctgaacc tccagctctg
     8641 ggctataagg ggataagtga atgagcgata tgagtgaagc gtcggttttt cccgtggagc
     8701 cggagagcgg aacaccagga cgccagtacc gtctcgccct tcgcggcaac agcctgaatc
     8761 caatgattga cgccgccacg ccgttgctcg gcatggtgat gcgtctgtcc actatgaaca
     8821 gtcaggccat gccggaacat ctgtttgccc aggtagtaac cgatgtccag gccgtagagc
     8881 aactactcca ggaacaggga tacgaaccgg gcgtcattgt ttcgttccgt tacattctct
     8941 gcacgtttat tgatgaagcg gcgctgggca acggctggtc caacaaaaac gaatggatca
     9001 aacagtcgct gctggttcat tttcataacg aagcctgggg cggcgagaaa gtgtttatcc
     9061 tgctcgaacg cctgatccgg gagccggtac gttaccagga tctgctggaa tttttatacc
     9121 tctgtttttc gctcggcttt cgcgggcgct acaaggttgc cattcagcaa caggatgaat
     9181 ttgagcggat ctaccagcgc ctgcaccatg ccctgcacaa actgcgcggt gacgcgccat
     9241 ttccgctact tcatcagaag aacaacatac agggtgggcg ttatcagtta atccgacgac
     9301 tgaccatcaa gcatgtactg tgcggcggtc ttgttgtgct gaccgtattt tatctgtttt
     9361 atctgctgcg tctggacagc cagacacagg atattctgca tcagctcaat aaattactcc
     9421 gataaggaca tcttatgatc cagattgatt tagccacgct cgtaaagcgg cttaacccct
     9481 ttgcaaaaca ggcgctggaa atggctgcct cggagtgtat gagccagcag gccagtgaga
     9541 tcaccgttgc ccatgtgctg ttacagatgc tggcaatccc gcgcaacgac gtaagggtga
     9601 ttgccgaacg cacaggcatc agtgcggagg atttacgcca ggcgctgacc gtggagagtt
     9661 atcccggtgg acgttccgcc gaaggctatc ccagcttctc cccgatgctg atcgaatggc
     9721 tcaaggagtc atggctgctg gcctcagccc agatgcagca cagcgaactg cgcagcggcg
     9781 tcctgctgct tactctgctg cattcaccgc tgcgctatat tccacccgct gctgcccgtc
     9841 tgctgacggc cataaaccgc gaccagttac agcaggattt cgcagcatgg acaaaagaat
     9901 ccgccgaatc cgtgaaccag gttggcggac aggcacccgg cacgcctgaa accggcgaca
     9961 gcgggcttgc ccgctacgcc aaaaacatga ccgaagatgc ccgtaacggc aaacttgacc
    10021 ctgtactgtg ccgcgaccat gaaatcgacc tgatgatcga cattctctgc cgccgtcgta
    10081 aaaacaatcc ggtggtggtg ggtgaagcgg gtgtgggtaa aagtgcctta atcgaagggc
    10141 tggcgctgcg tattgtctca ggccaggtgc cggacaagct gaaaaacacc gatattatga
    10201 cccttgacct cggcgcattg caggccgggg cgtcagtgaa gggcgagttt gaaaaacgct
    10261 ttaagggact gatggcggaa gttatctcgt ctcctgtgcc ggtcattctg tttatcgacg
    10321 aagcgcatac cctgattggc gcgggaaatc aggccggagg actcgatgtt tccgggttgc
    10381 tgaaaccggc gctggcgcgg ggtgaactga aaaccatcgc cgccaccacc tggagcgaat
    10441 acaaaaaata cttcgagaaa gatgcagctt tgtcgcgccg cttccagtta gtgaaggtca
    10501 gcgaacccaa cgctgccgaa gccaccatta ttctgcgcgg tttgtcggcg gtctatgaac
    10561 agtctcacgg cgtgctgatt gatgatgacg ccttgcaggc cgctgcgaca ctgagcgagc
    10621 gttatctctc cgggcggcag ttgccggaca aagccattga tgtgctggac accgcctgcg
    10681 cccgtgtggc catcaacctg tcatcgccgc caaaacaaat ctcggcactg accaccctga
    10741 gccaccagca ggaggcagaa attcgccagc ttgagcgcga gcttcgcatc gggctgcgta
    10801 ccgacacctc acgaatgact gaggtgttgg agcagtatga tgaaacgctg tcggcgctgg
    10861 acgaactgga agtcgcctgg cagcagcagc agcagacgct ggtacaggaa attatcgcgc
    10921 tgcgccagca gttgctgggc gcggcagaag acactgatgc tgtggtggaa gaagtcaccg
    10981 aaacagtttc cccggtgcag cggctggcac agcttactgc cgaactggac gccctgcata
    11041 acgaccggtt gctggtttcc ccgcacgtcg ataaaaaaca gattgcggcg gtgattgccg
    11101 aatggaccgg cgtaccgctt aaccgcctgt cgcagaatga gatgtcggtc atcaccgacc
    11161 tgccgaagtg gcttggtgac accatcaaag gccaggacct ggcaattgcc agcctgcata
    11221 aacacctgct gaccgcacgc gccgacctgc gccgtccggg acgcccgctc ggcgcgttcc
    11281 tgctggctgg ccccagcggc gtgggtaaaa ccgaaaccgt cctgcaactg gcagagctgc
    11341 tctacggcgg tcgccagtac ctgaccacca tcaatatgtc tgagttccag gagaaacaca
    11401 ccgtttcgcg gctgattggc tctcctccgg gttatgtcgg ctacggtgaa ggcggcgtac
    11461 tgaccgaagc ggttcgccag aaaccgtact cggtggtgct gctcgatgaa gtagaaaaag
    11521 cgcacccgga tgtactcaac ctgttctacc aggcgtttga caagggcgag atggcagacg
    11581 gtgaaggccg cctgattgac tgtaaaaata tcgttttctt cctgacctcc aaccttggct
    11641 accaggtgat tgtcgaacac gccgatgacc cggaaaccat gcaggaagcc ctctatccgg
    11701 tgctggcgga cttctttaaa cctgctctgc tggcgcgcat ggaagtggtg ccttacctac
    11761 cgctgtcgaa agagacgctc gctaccatta ttgccgggaa actggcccgc ctggataacg
    11821 tgctgcgcag ccgctttggc gcggacgtga ttattgggcc ggaagtgacg gacgaaatca
    11881 tgagccgcgt cacccgcgcg gaaaacggcg cgaggatgct ggaatcggtt atcgacggcg
    11941 acatgctgcc gccgctctcg ctgctgctgt tgcagaaaat ggcggccaac acggcgattg
    12001 cccggattcg cctgtcggcg gcagacggtg cgttcacggc agatgtggaa gatgcgctgc
    12061 ctgatgatgc cgtgacaccg caaacagagg acgaaacggt tttatgagca ggttgtgcgt
    12121 tttccggcag acaggatggt ttatcgcggg cctgatggcg ggcttacccg ccagtgccgc
    12181 gcccacagaa acatcgtcag tggccggtgt ggcggtagct gtcgccacaa cgacaccgcc
    12241 ggatgcgaca gccacgttac aggccatgca gtcctgccgc caggagacgg cggcgctgga
    12301 gcggcttgac tgttatgacc gcattcttgc gccggaacag gctggctttg gtggtgccgc
    12361 actggtgaaa gctcgttacc agggtgaagc ctgggcccgc gcgacagaac aggaaaaacg
    12421 ccgccagggc aacaccacgg agctgctggt aacgcaagta ccgggtgaac gcccgacggt
    12481 ggtgataacc accccggcta tcggccatgt tccgccacgc ccggtgctga tgttcagttg
    12541 cgtggacaat atcacccgga tgcaggtggc gctgatgcac cctctggatg tccatgatat
    12601 cgccgtcacc ctgaatgccg acagccgggc attgcgcagc cactggtttg tgcgcgagaa
    12661 cggcaccctg ctggagtcca gccgggggtt atccggtatc gatgaaatta agcaactgtt
    12721 cggcgcaaaa acgctgacgg tggataccgg cacagacaac gccgccggaa agctgacctt
    12781 taacattgac gggctggcac gggttattgc gccgctacgc gacgcctgcc actgggcggg
    12841 agaataagaa tatggcaatg gacttacgta atcccgatgt atggcttgca cacctgctgg
    12901 aaaacctgcc agaagataag ctgtccgccg cgcttgatga tggcaatgca gactgggagt
    12961 ttgtcgacag cgaaatcgtc aagctcggtt cactggcgca cagccagctt gatatcccgg
    13021 aacttcagcg ccggggcctg atgcttctgg cctcagaaac caaagatttt cgcctgctgg
    13081 cccacttgct gcgtaccctg caacatgccg gtgatattct gctggcctcc aggatgctgg
    13141 cacaatatac agagcactac tggacatgcg ccgcaccgca gaacatggcc cacaaaaaac
    13201 gctttgccgc ccaggtgata aaacgctttg agtctgccgt tcaggacttt gccgggaatg
    13261 ctgcgacaat acagcgcgat gctttgctgg gagagctggc aaaactggca cagtgctggc
    13321 aggcgcacaa cgcgtctgag ctggcaaagg cgacagacga tttattttcc ctgtttcagc
    13381 gcgcgtttcg tgatgtagcg cctgaaatga catcatctgc acgaagcgcc gccattgcgc
    13441 cgcaggccac gaccggatat gtaccggata tgtcaccgca cacggttgcc gctcagcctc
    13501 ctgttgcagc cactccaatc ccgcaggtca ccgttgaaag ccacgacgat aaagcatggc
    13561 gcgacaccct gctaaaggtg gccgccattc tctgtgagcg ccagccggag tcaccgcagg
    13621 gttaccgact gcgccgccat gcgctgtggc aaaccatcac cagtaccccg caggcagaaa
    13681 gcgatggccg gaccccactg gcagccttcc ctgtcgatat gatggatgat tacctggcgc
    13741 ggctgaacaa tgccgacatg gcgctgtggc agcaggtgga aaaaagcctg ctgctggccc
    13801 cgtactggct ggacggtcat tacctctcag cacagaccgc gctgcgcctc ggttataaac
    13861 aggtggcgga tgctatccgt gatgaagtta ccgattttct ggcccgcctg cccgcactga
    13921 tcaacctgtt gtttaacgac cgcactccgt ttgtgtccga acagacgaaa cagtggctgg
    13981 catcatccgg ctcagtcaac cagaccgtcc cggttgtaca gaccgatgaa gaattacagg
    14041 cggccaaggc gtgcttcgat gaaaacgggc tggaagccgc gctgcggtac ctggaaaacc
    14101 tgcccgaagg tgatccccgc caccagtttc accgccagtt tttcggcgcg cagttactgg
    14161 aagaggccgg tatggttcag cttgcacaac agcagtaccg gatgctcttc agaacaggat
    14221 tacacatgat gctatccgaa tgggagcctt ccctgctgaa agcactggaa caaaaactca
    14281 cggcagaaca gtaaaagata acaaggagtt actgtgttca aatttcccac atcccgactg
    14341 ttcagcacgt tgaaatctgc gctcaggcca gcgatgccgc ggtttaaagt ttccgccacc
    14401 tggctactga cgctggcatg gatttttctg ctggtgtgga tctggtggca gggtccaaaa
    14461 tggacgctct atgagcagca ctggctggct ccgctggcaa accgctggct ggcgaccgcc
    14521 gtctggggac ttatcgctct ggtctggctc acctggcggg tgatgaagcg tctgcaaaag
    14581 ctggaaaaac agcagaaaca gcagcgggag gaagaaaaag atccgttgac cgtggaactc
    14641 caccgccagc agcaatatct ggatcactgg ctgctgcgcc tgcgccgcca tctggataac
    14701 cgccgttatc tgtggcagtt gccgtggtat atggtcattg gtcctgcggg tagcggcaaa
    14761 agcacgctgc tgcgcgaggg ctttccgtct gacattgttt acacgccgga aagcatccgg
    14821 ggtgtggaat accacccgct gatcacaccg cgagtgggca accaggcggt aattttcgat
    14881 gttgacggcg tactgaccac tcccggcggg gatgatctgc tccgccgcct gcgcgaacac
    14941 tggctgggct ggctgatgca aacgcgcgct cgccagccgc tcaacggtct tatcctgacg
    15001 ctcgatcttc ccgatctgct gacggcggat aaatcccgcc gtgagacact ggtacaaaat
    15061 ttgcgccagc aacttcagga gatccgtcag agcctgcact gccgtctgcc cgtttacgtg
    15121 gtgctgacac ggctggatct gctgaacggc tttgccgcgc tgttccattc actggataaa
    15181 aaagaccgcg atgcgatcct cggcgtcaca tttacccgcc gcgcccatga aagtgacggc
    15241 tggcgcagcg aactgggggc tttctggcag acgtgggtac aacaggtgaa cctggcgctg
    15301 tcggatctgg tgctcgcaca aaccggtgct gctccccgca gcgcagtgtt cagcttctcc
    15361 cgtcagatgc agggaacagg agaaatcgtc accgcactgc tcgccgcatt gctggacggt
    15421 gagaacatgg atgtaatgct gcgtggcgtc tggctcacat cctcgctaca gcgtggccag
    15481 gtggatgata ttttcacgca gtccgccgcc cgccagtacg gactgggtaa cagctcgctg
    15541 gcaacctggc ctctggtgga gacgacgccg tattttactc gccgcctctt cccggaagtc
    15601 ctgctggctg agccgaacct ggcgggtgaa aacagcgtct ggctgaacag ctcccggcgc
    15661 aggctgaccg ccttttccac ctgtggcgcg gcactggcgg cattgatggt cggaagctgg
    15721 caccattatt acaatcagaa ctggcagtct ggcgttaacg tactggcaca agctaaagcc
    15781 tttatggacg taccaccacc gcagggaacg gatgaattcg gcaatctgca attgccattg
    15841 cttaacccgg tacgcgatgc caccctggcc tatggtgatt atcgcgatca cggttttctg
    15901 gcggatatgg gattgtacca gggcgcccgc gtagggccgt atgtggagca aacctacatt
    15961 cagcttcttg agcagcgtta tctcccctcg ttaatgaacg gcctgatccg ggatctaaac
    16021 attgccccgc cagagagcga agaaaagctc gctgtgctgc gcgtagtgcg catgatggaa
    16081 gacaaaagtg ggcgcaacaa cgaggcggta aaacagtaca tggcacggcg ttggagcaat
    16141 gaatttcacg gccagcgcga tattcaggcg caactgatgg tgcatctgga ctatgcgctg
    16201 gagcacaccg actggcacgc gcagcgccaa agcagcgaca gcgatgctgt aagccgctgg
    16261 accccctata ataaaccggt cattaatgcg caacatgaac tgagcaagct acccatatac
    16321 cagcgtgtct accagaccct tcgcaccaaa gcattaagcg tgttacccgc cgatttgaat
    16381 ttgcgcgacc aggttggtcc caccttcgac aacgtgttcg tcgccggtaa tgatgaaaaa
    16441 ctggtgatcc cgcagtttct cacccgctat ggcctgcaaa gctattttat caaacaacac
    16501 gacggtctcg ttgagttgac cgcgctggat tcgtgggtgc tgaacctgac gcaaagcgtc
    16561 gcctacagcg aggccgaccg tgaagagatc cagcgccata tcactgaaca gtacctcagt
    16621 gactataccg ctacatggcg tgccggaatg gataatctca acgtccgtga ctatgagacc
    16681 atgccggcgc tgaccgacgc gctggagcag attatcagcg gtgatcagcc attcctgcgt
    16741 gctctgacgg cgctgcacga taatacacac gcgctgacgc tctccggcaa actggacgat
    16801 aaggcgaagg aagcggcgat aaatgagatg gattaccgcc tgttatcccg gctggggcat
    16861 gagttcgcgc cggaaaatag cgcactggag gagcaaaagg acaaggcgag tactctacag
    16921 gccgtgtatc agcaactgac cgagctgcac cgttacctgc tggcgatcca gaactcgcca
    16981 gtgtctggga aatcggcgct gaaagcagta cagctacggc tggatcaaaa cagcagcgat
    17041 ccaatcttcg ccacccgtca gatggcaaaa accctgcctg cgcctcttaa ccgctgggta
    17101 ggtaagctgg cggatcaggc ctggcatgta gtgatggtgg aagccgttcg ttacatggaa
    17161 gtggactggc gcgacaatgt agtgaaaccc ttcaacgagc agcttgccga taactatccg
    17221 tttaatccgc gcgccacaca ggatgcctca ctggattcgt ttgaacgttt ctttaaaccg
    17281 gatggcattc tggacaattt ctacaagaac aacctgcgcc tgttccttga aaacgatctg
    17341 acctttggcg acgacggcag aatgttaatc cgtgaagata tccggcagca actggatact
    17401 gcgcagaaaa tccgcaacat cttcttcagc cagcagaacg ggctgggcgc acagtttgcc
    17461 gtggaaaccg tatcgctttc cggcaataag cggcgcagcg tacttaacct ggacggccag
    17521 ttagtggact acagccaggg acgcaactac accgcccatc tggtctggcc gaacaacatg
    17581 cgtgaaggca atgaaagcca gctgacgctg attggcacca gcggcagagc accgcgcagt
    17641 atcgcgttca gtggaccgtg ggcgcagttc cgcctgttcg gcgcgggcca gttgaccaat
    17701 gtgaccagtg acacctttaa cgtgcgcttt aacgtggacg gcggcgcgat ggtttaccgg
    17761 gtgcatgtgg ataccgaaga taacccgttc accggcggtc tgttcagcca gttccgttta
    17821 ccagatacgt tgtattaagg ggatagtgat gagcaataac gcactgacac aaactatcgt
    17881 taccggcagc gatccgcgcg ccctgccgga gttcagcgcc atccgcgaag aaattaacaa
    17941 agctaaccac ccgtcacagc cggagctgaa ctggaaactg gtggaatcgc tggcgctgtc
    18001 gatttttaag gccaacgggg tggatttgca taccgccacc tactatacgc tggcacgtac
    18061 acgtaatcag ggactggcag ggttctgtga aggcgcggaa ctgctggctg cgatgatcat
    18121 ccacgaatgg gataaattct ggccgcagag tggcccggcg cggactgaga tgctggactg
    18181 gttcaacacc cgcaccggga atatcctgcg ccagcaggtt tccttttccg aaaatgatct
    18241 ttcgttgctc taccgcacgg aacgggcttt gcagcttatc tgcgacaaac tccagcaagt
    18301 ggaactgaag cgccagccgc gcgtggaaaa tctgctttat tttgtgcaga acacacgcaa
    18361 gcggtttgag ccgcagccca gaaaccgtac tgatacggcg gcacagacca tggtcagaac
    18421 gctggtctat gcgcctgaag gcacggcttc cgccacagcg gaaaccatgc cccccctacc
    18481 cgacctgccg gaaatgcggg tgggggtgcg cggtgtggca gataacgccg acaaagcaaa
    18541 acagggcgac acgataaaag gttttgttgc cggtgccgcc tgtactgccg ttatcgcatc
    18601 tgcactctgg tggtggcagg cgtatccgat gcagcagcag cttacacagg tcagggatac
    18661 cacacaggga gccgccaccg tctggttggc ctccccggta ctgaaagaat atgaacagta
    18721 cctgcaacag cttctgaatg ccccgccact acagccgctg gaaaccggaa tgcagatgat
    18781 gcgcaccgca gacaccctct ggccggaaag cctgcaacag caggaagcca gcaggatgtg
    18841 gagcaacacg ctgagaaacc gggcgcaagc cagtccgcag atgaagggct ggcaacaagc
    18901 gcggcagaat ttgcgggatt ttgcagattt gatgatgaag aaggagacgg agaaacaagg
    18961 tttcacccta tcgtatatca aaacggtgac ctggcaggcc gaacggttac tgaatcaaga
    19021 aacaccactg gagtacctgc tgacgcagta ccaggagacc cgtacacaga agcaggacac
    19081 acaggcgttg gaaaaagaga tcaacgaacg gctggacggg ctgttaagcc gctggctgct
    19141 gctgaaaaat accggacagg atatggcaac ggataacaga accgaacctg tccatccaac
    19201 tcattgagat taaggagaat aacaatggca aatcccgtct atctgacact gaacggagaa
    19261 cttcaggggc tgatttcatc cggatgctcg tcaatgccgt ccatcggtaa caaagcccag
    19321 attgcacatc aggatcagat catggtgacg tcactgtccc atggtctgag ccgtgcgcag
    19381 aatgtcaacc atcaggagtt gaccatcacg aaaccggtgg acaaatcctc ccctctgctg
    19441 ggcaaggcca tttcagagaa tgaatgtctg acctgtgatt ttgtattcta ccgcaccaac
    19501 cgctttggaa ttaatgaacc gtactacaag ctgaaactgg ctaacgcccg gctctcaaac
    19561 atcggcctta ccgttcccca taccattaac gacagtcctg ggcaggcgga agagtctgtg
    19621 tcatttacct atgagagcat caattgggag cacagcgtcg ccggaaccag tgcatacagc
    19681 ctttggagtg aacggatttt ctaagttacc agggagtctt tcatctgcat tattgttata
    19741 ttttaaaaag gcaatttaac attataggat aaaatgacaa agtacggtta atcacgaata
    19801 aaaattctta attaaccgta cctgctgttt tatagatgta tatgatg
//
LOCUS       DAZFFE010000088        19736 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFFE010000088 DAZFFE010000000
VERSION     DAZFFE010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.088"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..80
                     /locus_tag="ACHJ69_003441"
     CDS             <1..80
                     /locus_tag="ACHJ69_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFO5016356.1"
                     /translation="FGLLTYLTNLYFKIREDRRKAARGE"
     gene            85..420
                     /locus_tag="ACHJ69_003442"
                     /pseudo
     CDS             85..420
                     /locus_tag="ACHJ69_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
     gene            588..1073
                     /locus_tag="ACHJ69_003443"
     CDS             588..1073
                     /locus_tag="ACHJ69_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016357.1"
                     /translation="MKEHLQRPEEKRRDEQFAAFDDYCRKVMSRNLAECFSIHNDNFS
                     ELEWECNRPSFVVSGDAGKITISENGKVTPPSHQHSEELIEFAIDYLKNNKKQGLMKR
                     VGRCMGYLQVAAEIEALASGADKDAIVREALLRDFNTPPFKKVPAYWLHPGLTYLKVR
                     I"
     gene            1110..1643
                     /locus_tag="ACHJ69_003444"
     CDS             1110..1643
                     /locus_tag="ACHJ69_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5016358.1"
                     /translation="MNTKIKYGLSAAVLALISAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFSSTFYRKLNAGDRKGACAEIRRWIYDGGRDCRNRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1917..2486
                     /locus_tag="ACHJ69_003445"
     CDS             1917..2486
                     /locus_tag="ACHJ69_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO5016359.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKHEIRDKVIRYQEEC
                     DDVLYEYWTKGFAVNPRKMSAMEELNRACADMKRDKNIASLFGTGLNEWKLVKAAHVS
                     KIRTLINEANLLIDFVLADTGKGKITKAD"
     gene            2486..2632
                     /locus_tag="ACHJ69_003446"
     CDS             2486..2632
                     /locus_tag="ACHJ69_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016360.1"
                     /translation="MGRLMISDKLITLVKNLCVIVGISFSLMLVAIFFSTAWRVLTLS
                     GLVG"
     gene            2640..3134
                     /locus_tag="ACHJ69_003447"
     CDS             2640..3134
                     /locus_tag="ACHJ69_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO5016361.1"
                     /translation="MSRVLCVVIIVLAGGCGALWLATDHYRDNAITYKAQRDKKASEL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRVNATCPGT
                     VREATGTSGVDNATGPRLADTAERDYFILRERLMTMQKQLEGAQEYIRTQCTKLAFYY
                     PEDT"
     gene            3131..3355
                     /locus_tag="ACHJ69_003448"
     CDS             3131..3355
                     /locus_tag="ACHJ69_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016362.1"
                     /translation="MKKLRVTVEPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCHMVSAALQPVC"
     gene            3700..4026
                     /locus_tag="ACHJ69_003449"
     CDS             3700..4026
                     /locus_tag="ACHJ69_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFO5016363.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
     gene            complement(4158..4358)
                     /locus_tag="ACHJ69_003450"
     CDS             complement(4158..4358)
                     /locus_tag="ACHJ69_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5016364.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMKNNEK"
     gene            4400..4765
                     /locus_tag="ACHJ69_003451"
     CDS             4400..4765
                     /locus_tag="ACHJ69_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO5016365.1"
                     /translation="MAQLRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCICGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
     gene            5057..5620
                     /locus_tag="ACHJ69_003452"
     CDS             5057..5620
                     /locus_tag="ACHJ69_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5016366.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQGETVEVDEVPVYRQKKTQTEECHQEAPPAEKNTPVSLPPDDRQSLTPL
                     PGIDYMEDGLPDPVKAMGRILVENLSIDPKLALDAAWRLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            5617..7278
                     /locus_tag="ACHJ69_003453"
     CDS             5617..7278
                     /locus_tag="ACHJ69_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO5016367.1"
                     /translation="MIPGWSTACPDWAVRLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGEHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRISLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVA
                     RRKSEAARFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGDQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            7342..9279
                     /locus_tag="ACHJ69_003454"
     CDS             7342..9279
                     /locus_tag="ACHJ69_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO5016368.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFMRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVAANLQP
                     TGAIWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGTASGG"
     gene            9324..9545
                     /locus_tag="ACHJ69_003455"
     CDS             9324..9545
                     /locus_tag="ACHJ69_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016369.1"
                     /translation="MAKIQYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            9491..11590
                     /locus_tag="ACHJ69_003456"
     CDS             9491..11590
                     /locus_tag="ACHJ69_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5016370.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPESVLSFHAVFSCISLISQDIAKMRLRLMQTDTQGIRREKRQGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNSRGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLVGLPPVYAAGLAATQGHHIQANSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGESVKNTLLTPNEARKRENLPPLAGGDAL
                     YLQQQNYSLEALSRRDAREDPFASSGKTSSGTRQEGASDGNKAISETEHDAVKAMFRG
                     DTEKMTERELSIIRALGEEFAAVLADLQRTFEGKIASQAQMFEEKLASLSVVLQKCVT
                     GDDVRPMLEQMVKEAVSHIPVPRDGRDYDPDVLQKAVNDAVSALPAPQDGRDATALEI
                     LPAIDDQKSFPRGTYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVV
                     VRQSSGQRTEKTFSLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTEDKPGEAHS
                     SAWTLAAKRGRDAGGGK"
     gene            11587..11913
                     /locus_tag="ACHJ69_003457"
     CDS             11587..11913
                     /locus_tag="ACHJ69_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO5016371.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDEMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            11923..12273
                     /locus_tag="ACHJ69_003458"
     CDS             11923..12273
                     /locus_tag="ACHJ69_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO5016372.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGLSGREITAASRLHVLSGPWRDCILNVVGVPVPDAT
                     GGRLEILCRLGGEK"
     gene            12270..12716
                     /locus_tag="ACHJ69_003459"
     CDS             12270..12716
                     /locus_tag="ACHJ69_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO5016373.1"
                     /translation="MIETLLDFSGLEEISRDLQLLSGAENNRVLREATRAGANELKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            12713..13057
                     /locus_tag="ACHJ69_003460"
     CDS             12713..13057
                     /locus_tag="ACHJ69_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO5016374.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            13124..13840
                     /locus_tag="ACHJ69_003461"
     CDS             13124..13840
                     /locus_tag="ACHJ69_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO5016375.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            13855..14229
                     /locus_tag="ACHJ69_003462"
     CDS             13855..14229
                     /locus_tag="ACHJ69_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO5016376.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            14325..14534
                     /locus_tag="ACHJ69_003463"
     CDS             14325..14534
                     /locus_tag="ACHJ69_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO5016377.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            14582..17824
                     /locus_tag="ACHJ69_003464"
     CDS             14582..17824
                     /locus_tag="ACHJ69_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5016378.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AVAREQRRALAELNSQLTEIRASAAGTAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYTADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGVLRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     VTKTLSGVLNGVAGNIDNVATAAGALVAVGVARYFGNMASGAVSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAART
                     AAQNALNSTTAVGSRLMTGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALAEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLSQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALTKTERELALSRLKGEEKERARLGYAADDLGFVGDSYQEARQRYISNALEAW
                     RNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTAQGELKT
                     LTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQGERARE
                     RMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQARAQNAQYLSERLKDQAAFYAES
                     DAQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNKVEWRNW
                     ASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFIGSIGDWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLV
                     YSPVYHIAIQNDGANGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            17817..18158
                     /locus_tag="ACHJ69_003465"
     CDS             17817..18158
                     /locus_tag="ACHJ69_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5016379.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEATRRWLDEFLSWHGGYRAFLWKPPKHNRTVRVVCREWSITDNARYS
                     DFSCMMEQVIR"
     gene            18365..19528
                     /gene="ompC"
                     /locus_tag="ACHJ69_003466"
     CDS             18365..19528
                     /gene="ompC"
                     /locus_tag="ACHJ69_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO5016380.1"
                     /translation="MKKMTVALSAVAAAVIFAAGAQAAEVYNKDGNKLDVYGRATALH
                     YFSSDKGDDGDKTYARLGFKGETQINDQLTGFGQWEYQFSGNKAESEGAAGNKTRLAF
                     AGLKFGEFGTIDYGRNYGIAYDVGSYTDVLPEFGGDGWTQTDNFMTARTSGVLTYRNT
                     DFFGLVDGLNFAAQYQGKNDRGDLRKANGDGHGFSASYEYEGFGIVAAYINADRTNNQ
                     EKGLNGTLTIEQTDPLTGKVTEKQIVVDSGSVAKGKHAEMWGVGLKYDADNLYLATTY
                     SETQNLTTFGDKGVADKAQNFEAVLQYQFDFGLRPSLAYLQSRGQDVMVGGVNHGDQD
                     LVKYIDVGATYYFNKNMSTYVDYKINLIDESEFTRKAGVATDNIVAVGMTYQF"
BASE COUNT         4671 a         4563 c         6119 g         4383 t
ORIGIN      
        1 tgtttgggct gctgacatat ctgactaacc tgtatttcaa aatcagagag gaccgtcgta
       61 aggcggcgcg gggagagtaa agcgatgaag aaaaaatacg aactggttgt taaagggata
      121 aataattacc cgaataagat tactgttact gtggcactgg aaattggtgg gtatccgtca
      181 ctgttgttgc cagatgtggc gattagtctt gaccgtactg aagatgccac gctggagttt
      241 tacgaagctg aggcgaaaaa gcaggcgaag cagtttttca tggatgttgc tgccgggtta
      301 tgtgaaggga acgaaccgtt gccggaaaag cgccccgtaa ttttagaggc gcaggatgtg
      361 ttgataacct acaaaggaaa gctaccggga agaattactg atgctctgaa gatgccgccg
      421 tcaacactgc ggtcagaaaa agatgatgtt gaatcacgta ttgaaaaact ggagcgctat
      481 atcgctgaat tgaaaaaaag cgccccaaca aaaaatgagg tgcttgcagc agatgaaatg
      541 aaagaagcta ttcttgatcg cgcgggcatc taagctgcgc ttcacggttg aaagagcatc
      601 ttcagcggcc tgaagaaaaa cgccgcgatg agcagtttgc tgcgtttgac gattattgtc
      661 ggaaagtgat gagcagaaat ctcgcagagt gtttcagtat tcataatgat aatttcagtg
      721 agctggaatg ggagtgtaac cggccatcct ttgttgtatc cggtgatgcc gggaaaataa
      781 ccatctcaga aaatgggaaa gtaacacctc catcgcacca gcacagtgag gagctcattg
      841 aatttgccat tgattacctg aagaacaata aaaagcaggg gctgatgaag cgcgttggcc
      901 gttgcatggg atatcttcag gtagccgctg agattgaagc gctggccagt ggtgctgata
      961 aggatgcaat tgtgcgggag gctcttcttc gtgattttaa tactccaccc tttaaaaaag
     1021 tgccggctta ctggcttcat ccggggctga cttatcttaa agtgcgtatt tagtgggcca
     1081 gggacagcgg ctgaatattt aatatatcca tgaacaccaa aatcaaatac ggcctgtcgg
     1141 ctgccgttct ggcgctgatt tccgctggtg cgcctgcgcc tgacattctc gaccagtttc
     1201 tggatgaaaa ggaaggcaac cacaccacgg cataccgtga tggtgcaggt atctggacca
     1261 tctgccgtgg tgccatcatg gtggatggca aacctgtcgt tccgggcatg aagttgtcga
     1321 aggaaaaatg cgaccaggtt aacgccattg agcgtgataa agcgctggcg tgggtggaga
     1381 aaaacatcag agtgccgctg accgaacccc agaaagcggg gatcgcgtca ttctgtccgt
     1441 acaacattgg tcccggtaag tgtttcagct caacgtttta ccggaaactg aacgccggag
     1501 accggaaagg tgcatgtgct gaaattcgtc gctggatata tgacggcggc agggactgtc
     1561 ggaaccgctc aaataactgt tacgggcagg tatcgcggcg tgaccaggag agcgcgctgg
     1621 cgtgctgggg tatcgacagg tagcagaata ttttgctgaa aaatgacgct tgctcacgcg
     1681 ggcggataac acgaaatcct gcgaactggc aaaatgcaag tgaataaagt caggaagatt
     1741 gtttcacgca gaggcaccgt aatggtgtct ttgtcatttc tgcgcttcgc acaagcgtaa
     1801 ataaaccaaa gaacctttca ggatgagccc tggtggataa ccggcagtgg tctggttaac
     1861 cctctttggg ctggttattc ctgtgcgcag ggttcatcac taaaaggaaa taaccgatga
     1921 atatgatgac cgtgccgttt cacggcgatt ctctttatgt ggttaaccat aacggcgaac
     1981 catacgttcc catgaaacct gtcgttgcgg ggatggggct ggcctggcaa tcacagttgg
     2041 ctaagttaag acagcgtttt gcgtcaacta taacggaaat cgttatggtt gctgaggatg
     2101 ggaaacgacg caatatggtg tccctgccac ttcgaaaact tgccggctgg ctacaaacca
     2161 ttaatcccaa caaagtaaaa cacgaaatcc gcgataaggt catccggtat caggaagagt
     2221 gcgacgatgt tctttacgag tactggacga agggttttgc cgttaatccc cgtaaaatga
     2281 gcgcgatgga agaactcaac cgggcatgcg ctgacatgaa acgggataaa aacattgcca
     2341 gtctgtttgg tacagggtta aacgaatgga aactggttaa agccgcgcat gtatcaaaaa
     2401 ttcgcacgtt gataaacgaa gcgaatctgc tgattgattt tgtcctggct gatacaggca
     2461 aagggaaaat aacaaaggcg gattgatggg gcggctaatg atatcagata aactcataac
     2521 actggtgaag aacctctgtg taattgtcgg catttcattt tcactcatgc tggttgccat
     2581 tttcttttcc accgcctggc gagtcctgac gttatcggga ctggtggggt gaaagagaga
     2641 tgagccgtgt tctgtgtgtg gtgattattg tcctggcggg tggctgtggt gcgctgtggc
     2701 tggcaacaga ccattaccgt gataacgcca tcacctacaa agcgcagcgc gataaaaaag
     2761 ccagtgagct ggagctggca aacgcaacca ttactgacat gcagcagcgc cagcgtgatg
     2821 ttgctgcgct tgatgccaga tactcaaggg aattagccga tgcgagagct gaaaatgaaa
     2881 ctctgcgcgc tgacgttgcc gctggtcgta agcgcctgcg ggtcaacgcc acctgccccg
     2941 gtaccgtgcg tgaagccacc ggcacctccg gcgtggataa tgcaaccggc ccccgactgg
     3001 cagacaccgc tgaacgggat tatttcatcc tcagagaacg gttgatgaca atgcagaagc
     3061 agctggaagg ggcgcaggaa tatatccgca ctcagtgcac taagctggct ttttattatc
     3121 cggaggatac atgaagaaat tacgggtaac cgtagaacct tttcagggaa caattccgtt
     3181 ccgtattttg cagcgtggtc gtgttcttgt tgaaggttcg ttcagtggta aatgtacgca
     3241 attacactcc cggacctttc aggtgaatgc cacgaatgaa gagctaaccg tggagtgtac
     3301 gatgaatgcc gctaaatgcc acatggtatc cgctgcatta cagccagtgt gttgagcgac
     3361 cttattatcc atgcgcggta ttgtcgccgt attcctgcat taacagagac cgcagcccga
     3421 ccgggagact cctctgcgcg agtgtgcggg gataatcaaa aacgatacac accggggttt
     3481 accgcgttaa cggagcgcgg cgttgtcccc tcatggtcgc tggtccggtg cgatggtgga
     3541 agaaaccgga cgatgtgtta cctcgcaagc tctgttatgt catgtgtctg atttgtgatt
     3601 taagtcggat aattgtcgtt gccattaaac agaggattga tgaccgacag ggtggcattg
     3661 ttagaataag acttattctt atctgtgccg ggaatgaaaa tgaaaagaaa tcttccgtta
     3721 attattttgt tgtcttctct ggttatgggc tgtacgcaac ataaaacaga tatgccccga
     3781 cagttggtta aggcattacc acaatatccg gcctatgcag cggcaaatta tataaaggga
     3841 cgggttgatg tgaagtttga tattggtgct gatggtactg tcacccgaat tgagtttatc
     3901 cgttcagagc cgcaccattt gtttgatgag caggttgtaa aggcgatggc aaaatggcga
     3961 tttgagaagg acaggccgtg taaaggcgtg aagaaaacgt ttatctttag tccttctgca
     4021 ccctgattat ttcatcagaa attaattatc actctgttgt tattctgtac atcccggcag
     4081 ggtaagtctt gttccgtcgg atatgaagat gaaatattgt tggaggacag tgggtacctg
     4141 ctcctgtaac cgaacgttca tttctcgtta tttttcatgc tggccgggcg cagatgcgtt
     4201 gcatctgttg ccagccttct cctgcaggct tcaataaccc acgctgaaaa gttaccggat
     4261 cctttatgct caagggcgat gttgatctgt tcaatcatgt gattggggaa acggatattg
     4321 cgggttgtgg ttctgcgggt ccggtttttc gatgacatat ttatttcctt tactgattgc
     4381 catatgacgg ggattttaca tggctcagct tcgtacactc cagagcagaa tcaaaacact
     4441 gaatacccga cgggtgaata ttctgaaggg ggagcagcgt cgtgtcagtg gcagtgcacg
     4501 tgtttccctc aagcgtcata tctggctgag ggacgccggg cagtgctgta tctgtggtcg
     4561 tgtggttgac ctctgtgaca gtgaactcga tcaccgaatt gcacttcagt tcggtggtgg
     4621 taatgaggag acgaatctct ggacgctctg taccgaatgc catcgacaaa agtctgctcg
     4681 tgaagcggcg ggtggtatgc cggacccgac gctgccggag gtgtccggag gtagtggcag
     4741 agcggacgac atcatcggac tgtaacccga cccggggggg gtatcatccg gcataaaaaa
     4801 acgatcgctt tggacaccgc gccccgtctc acgcagagaa aaaattcccg tttcaggcca
     4861 gttaacatgt taactggctg tccgggcatt ttttcggttt ttatctttat tattcagttt
     4921 gttgcatgga aaaaatgtta actggctttt tcagcaaatg ttaaccaggc agcagttaac
     4981 atttgcggca tgaggcgccg ggaaaaatgg gctgaaccat acccggctga gtgcgttctg
     5041 gacccgggag gaggctgtgc tgacaacgca aaaacgaaaa tttgcgctgg cgctcatgtc
     5101 cgggaaaaac aaaacagcgt cagccattgc cgccggttat tcggcgaaga ccgccagggt
     5161 taaaggctcg cagctggcaa aagatccgga ggtgctcgcg tttattgccc gtaagcaggg
     5221 cgagacggtg gaggtggatg aggttcctgt ttaccggcag aaaaagacgc agacggagga
     5281 gtgccatcag gaagcccctc cggcggaaaa gaacaccccg gtttcactgc cgcctgatga
     5341 ccggcaaagt ctcacaccac taccaggtat tgattacatg gaagacggtc ttcctgatcc
     5401 ggtaaaagcg atggggagga tcctggtgga aaacctgagc attgacccga aactggcact
     5461 ggatgcggcc tggcgactgg cacagttcac gcaccataaa aaaggggatg ccggtaaaaa
     5521 atcggcaaaa ggtgacgcgg cgaaaaaagc ggctaaccgt tttgcggtgc caccaccacc
     5581 ccgcctggtg gtgaataacg ataatgaggg caacggatga taccagggtg gagcacggcc
     5641 tgcccggact gggcagtacg cctgaaaaag gggctgtcga ttattccggc tccgatttac
     5701 ccggagcagg ccgcacatgc cctggcgatt tttaaacaac tgcggattgt ggatgcaccg
     5761 ggcagcccga cgttcgggga gtcctgtgca cagtgggtgt ttgacctggt ggcggccctg
     5821 tttggctcct acgatgcgca gaccggtgtg cgccatatca aggaagtgtt cattctgata
     5881 cctaaaaaaa acagcaagtc cacgctggcc gccgggatca tgatgacggc gctgttactg
     5941 aactggcggc aggcggcggg ctacaccatt ctggccccga ccgtggaggt ggcagccaac
     6001 gccttcaacc ctgcccggga tatggtacga cgcgacgatg atctggatga cctctgccag
     6061 gtacagacgc atatccggac catcacccac cgggtgacgg acaccaccct gaaggtggtg
     6121 gcagccgatc cgaatacggt gtccggtatc aagtccgtgg gtacgctgat tgatgagtta
     6181 tggctgtttg gcaaacagta caaggcggag gacatgttac gtgaagccat aggcggcctt
     6241 gcctcccgcc cggaagggtt tgtggtgtat acgaccaccc agtcgaatga gccgcctgcc
     6301 ggggtgttca ggcagaaact gcagtacgcc cgggatgtgc gtgacggcaa aattcatgat
     6361 ccgcactttc tgccggtgat ttttgagcat ccgccggaga tggtggagag cggtgagcac
     6421 ctgctgatgg aaaacctcgc catggtcaat ccgaatctcg gttattcggt ggatgaggcg
     6481 tttctgtacc gggagtaccg taaagcccga gaagccggtg aagagacttt tcgcggtttc
     6541 atgtcaaaac acgccaatgt ggaaattggt cttgccctgc gttctgaccg ctgggcgggg
     6601 gcggatttct gggagcagca gggcaggcgc atcagtctgg acgatattct gcagcgtgct
     6661 gatgtggtga cggtggggat tgacggcgga gggctggatg atctgctggg gatgtatgtg
     6721 attgggcgtg accgggagac ccgcgaatgg ctgggctggg gccatgcctg ggcacatgaa
     6781 accgcggtgg ccagacgaaa aagtgaggca gcccgttttc aggattttgt tgcctgtggg
     6841 gatatgacca ttgtccgtcg ggtcggggat gacacggcgg aagtggcgga gtatgtgcgt
     6901 cgcattcatg aggctgagtt actggaccat atcggtattg acccgtcagg ggtggggcag
     6961 attctggatt cactggcgga agccgggatc cccgacggaa ttgtggtggg gataagccag
     7021 ggctggaaac tgggcggggc cattaaaacc accgagcgca aactggctga aggggtgctg
     7081 gtgcatggtg accagcccct gatggcctgg tgtgtcggca atgcccgggt ggagcctaaa
     7141 ggtaacgcca ttcttatcac caaacaggcc agtggacggg gaaaaattga cccgctgatg
     7201 gcgctgttca atgcggtctc cctgatgtcc cttaacccgg aaccgaaaaa gaaagaatat
     7261 gcggtttttt tcatataacc ctgttcacac tgtaaccatc acgaaccgct ccggcggttt
     7321 ttttattttc aggaggctga tgtgactctt aaacgggcct gttccctgct gacggtgaaa
     7381 tccttcagtg aggatgaacg ggtgatcacc gggattgcgt caacgccttc tccggatcgg
     7441 gatggtgaca tcctggagcc ggagggcgcg gagtttggca gtgcgatccc gtttctctgg
     7501 cagcatgacc attcccgccc ggtggggcag tgtacggtgc gccgggtcag cgaagggctg
     7561 gaaatcacgg caacactggt gaagcccgta ccggatatgc cgtcgcaact ggctgcccgg
     7621 ctggatgagg tctgggcggc cattaagacc gggctggtca gggggctgtc cgtgggcttc
     7681 cgtccccatg aatacacctt tctggacgga ggcggactgc attttatgcg ctgggaactg
     7741 atggaggtgt ctgccgtcac cgtgcccgcg aatgcggaat gcaccatccg gaccattaaa
     7801 tcttacgacc gcccgttttc tgccgcgtcc ggcaaccgga aaccggtggt gaaaatcgca
     7861 tcttctgccg gcgctgcggc acagtcaaca accgtttttc ataaggaaaa gaccataatg
     7921 aatattggcg aacagattaa aagttttgaa aacaagcgtg cagcgctggc agcctccctt
     7981 gaggaggtca tgaccaaagc cgcagaggaa gggcgcacgc tggatgtgga ggaggaagag
     8041 cattacgaca acaccgcagc ggaaatccgt caggtggatg cgcacctgaa gcgcctgcgt
     8101 gaactggaag ccggtaaggc cgccacggcg cagccggtga aacaggccgg taacgggaat
     8161 gtggccgcgg tggcttctgc gccggtgatc cgtgtggagc agaaactgga taaggggatt
     8221 ggcttcgccc gctttgccaa atcgctggct gcggctaaag gcgtccgatc tgaagccctg
     8281 gaagtggccc gtcgtcagta tccggatgac agtcgtctgc atcatgtcct gaaatcggca
     8341 gtgggcgcgg ggaccaccac ggatccgcag tgggcaggca gcctgtctga atatcaggaa
     8401 tacgcacagg actttattga ttacctgcgt ccgcagacca ttatcgggcg atttggtcag
     8461 ggcgggatcc ctgcacttcg tcaggtgccg ttcaatatcc gtgtgcacgc ccaggtgtcc
     8521 ggcggtgctg ccggctgggt gggtgagggt aaggcaaaac ccctgacgaa gtttgatttt
     8581 gaatccatca ccttcagtca tgcgaaagtg tcggccattg cggtactgac ggaagaattg
     8641 atccgttttt ccagtccggc tgctgatgca ctggtccgta atgcgctggc ggaagcggtg
     8701 gtggcgcgtc tggatacaga ctttgtggac ccgaaaaaag ccgcagtggc agatgtctcc
     8761 ccggcgtcca tcacccatga tgtgaagggc acggcatcaa ccggtaaccc ggatgcggat
     8821 gcagaggctg cgtttggaca gtttgtggca gcaaacctgc agcccaccgg tgcgatctgg
     8881 ctgatgtcca gcaccaatgc cctggcactg tccatgcgta aaaatgcgct gggtcagaag
     8941 gaatacccgg acatgaccct gctgggtggc tccttccagg ggctgccggt gattgtctcc
     9001 cagtacgtgg gtgaccagct ggtgctggtg aatgccccgg atatttatct ggcggatgac
     9061 ggcggcgtgg cagtggatat gtcccgcgag gcatcactgg aaatgcagtc tgagccgacc
     9121 ggcgacagta ccacgccgtc cccggtggag ctggtttcca tgttccagac aggcagcgtg
     9181 gccatccgtg cggagcgctg gatcaactgg cgtcgtcgcc gtaccgcggc agtggcggtg
     9241 atcaccggtg tgaactacgg aactgcgtcc ggcggctgag tctgatgagg agggcgggag
     9301 gcgcgagctt cccgcagtaa ctgatggcaa aaatccagta tctgcagggc acgcatgatg
     9361 cccgggccgg ggatatccgt gatgtggcac agccgtgtgc ggaggtgctg gttcgcctgg
     9421 ggaaggcgga gtacatcacg gtgcgacgtc cggcaggtca gaaaaagaaa cgtgatgcgg
     9481 agcatggcga atgtggaacc ttttacggcg aacccgaaaa aaccagaaat caggacgtga
     9541 cgtaagagag gcgggctgga ccagcctgtt tcaggcggtg gctgagccct tttccggcgc
     9601 ctggcagcag ggtgtgaaag ccgatccgga aagtgtcctc tcctttcatg cggtgttttc
     9661 ttgcatttcg ctgatatccc aggatatcgc caaaatgcgg ctgcgcctga tgcagacgga
     9721 tacacagggg atccgccgtg aaaaacggca gggggatatt gcccgtctct gtcgtcgtcc
     9781 caatgcacag cagaatcgta tccagttttt tgaactgtgg ctgaacgcca aactgcgtca
     9841 cggcaatacg gtggtgctga aaatccgtaa ctcccggggg cagatcaaag aactgcgtat
     9901 tctggactgg agccgggttg aacctctggt ggcggatgac ggcgaggtgt tctatcgcat
     9961 tacaccggac cggaactgcg ggatcacgga ggcggtgacg gtgcctgcca gggaagtgat
    10021 ccacgaccgg tttaactgtt tttttcatcc gcttgtgggg ctgccgccgg tgtatgctgc
    10081 cgggctggcg gccacgcagg ggcatcatat tcaggcaaat tcaacgtctt ttttcagaaa
    10141 tggcggcagg ccgtccgggg tgattgagat ccccggcagt ataacggaag aaaatgcgaa
    10201 aaaactgaag agcaactggg acagcgggta taccggcgaa aatgcgggga aaacggcgat
    10261 cctgagcaac ggggcgaaat ataaccccac gacgttttca ccggtggatg cgcagacggt
    10321 ggaacagctg aaaatgacgg ctgaaattgt ctgttcggtg ttccgtgtcc cggcctacaa
    10381 gattggcgtt ggccagccgc cttccagtga caacgtggag gcgctggagc agcagtatta
    10441 ttcccagtgt ctgcagacgc tgattgagtc cattgagctg ttactggatg aggcgctgga
    10501 aacaggggaa aacgagagca cggagtttga cgtcaccacg ctgctgagaa tggacagcga
    10561 acgtcgcatg aaaacgctgg gtgaatcggt gaaaaatacg cttctcacgc ccaatgaggc
    10621 ccgtaaacgg gagaatctgc cgcccctggc aggaggcgat gcactgtatc ttcagcagca
    10681 gaactacagt ctggaggcgc tgtcccgtcg tgatgcccgt gaggatccgt tcgcgtcttc
    10741 cgggaaaacg tcgtcaggca cacgccagga gggcgcgtct gacggtaata aggccataag
    10801 cgaaacagag catgatgcag tgaaagcgat gttcaggggg gatactgaga aaatgacgga
    10861 acgggaactg tccattattc gtgcactggg agaagaattc gctgcggtgc tggcggattt
    10921 acagcgcaca tttgagggga agattgcctc gcaggcacaa atgtttgaag aaaaactggc
    10981 ttccctgtct gtggtattac agaagtgcgt gacgggcgat gatgtgcgtc cgatgcttga
    11041 acagatggtg aaggaggcgg taagccatat tcctgttccg cgtgatggtc gtgactacga
    11101 tccggatgtt ctgcagaagg cggtgaatga tgcggtgagt gccctgccgg ctccgcagga
    11161 cgggcgtgat gccacggcac tggaaatact ccccgccatt gacgatcaaa aatcctttcc
    11221 ccggggcacg tatgccacac accagggcgg actctggcgg gcgtatgaaa aaacgcacgg
    11281 gatgcgggga tgggaatgcc tggttgacgg ggtggcggat attgacgtca gcatgacggg
    11341 tgaacggttg ttctctgtgg tggtccggca gagcagtggc cagcgtacgg aaaaaacatt
    11401 ttccctgccg gtgatgctct accgcggtgt gttcagagcc ggtgaaacct accaccccgg
    11461 cgatacggtg acgtgggggg gctcgctgtg gcactgcaac agtatgaccg aagataaacc
    11521 cggagaagct cattcatcag cctggaccct ggctgcaaaa cgtgggcggg atgcaggagg
    11581 cggaaaatga cggcattact gacactggag gagatcaagg cacatctgcg tgttgaccat
    11641 gacgcggatg atgagatgct gatggacaag gttcgtcagg ctaccgccgt gctgctggcc
    11701 tacattcagg gcagccggga taaggtgatt cgtgaggacg gtgaactgat cccgggtgag
    11761 gcattaaccc ggatgaaggg ggctgccatg cgactgaccg ggatgctgta ccggaatccg
    11821 gatcttgcgg agcgggaaga cctcgtgcag ggggagttgc cgttttctgt ttccgtgctg
    11881 atttacgatt tgcgttgtcc gacggtgtta tgaggagggg gaatggcaat atctgcaggt
    11941 cgtctgacac agatgataag tgttctgaac ccggtgttaa cccgtaatgc tgccggagaa
    12001 atgacggaag aatgggtgtc atgcgggaaa attcatgcgg atatccgggg caggagcagc
    12061 cgggagcgga tgcagtccgg tgcggaaatg gcgcaggcgg aaatccgcat ctgggtgcgc
    12121 ggtctgtctg gtcgtgaaat cacggcggcg tcacgacttc atgtgctgag tggtccctgg
    12181 cgtgactgta tcctgaatgt tgtcggggtg cccgtgccgg atgcgaccgg cgggcgcctg
    12241 gaaattctct gtcggctggg aggggaaaaa tgatcgaaac cctgctggat ttttcgggac
    12301 ttgaagaaat aagccgtgat ttgcagcttc tgagtggtgc agaaaacaac cgggtactgc
    12361 gtgaggcaac ccgcgcgggg gcgaatgaac tgaaagaaga agtggtgtca cgggcaccgg
    12421 tgcgcagggg aaaactgcgc cgcaatgtgg tggtcctttc ccggcgctcc cgcgatggcg
    12481 ggatggaatc cggtgtgcat atccgtggtg ttaatccgga caccggcaac agtgataaca
    12541 ccatgaaggc ggataatccc cgcaatgcct tctactggcg gtttgtggaa atggggaccg
    12601 tgaatatgcc accgcacccg tttgtgcgcc cggcgtttga tgtgcgcagt gaacaggcag
    12661 ctcaggtggc gattgcgcgg atgaaccggg ccattgatga ggtactgaga cgatgacgga
    12721 ggcggatttg tatcctcatc tggcgcatct tgccggcggg caggtgtacc cgtatgtggt
    12781 ccccctgctg gatggcaggc cgtcggtggc gcttccgtgg gtggttttca gcctgatttc
    12841 atcggtgtca gcggacgtga tgggcgggca ggcggagtcc tctgtgtcgg tgcagataga
    12901 cgtttatgcc gggactgtga cgcaggcgcg tcagatacgt caggacgccc gtgaagccat
    12961 aatgctgctg gccccggggt ccgtcagtga aatgcaggac tatattccgg aaaaccgctg
    13021 ttaccgtgca accctggagt ttcaggtcac ggtgtgactt tttctttttt tctacaaaac
    13081 ccataccccg ccgcgtgcgg gttttttatt atcaggaggc agaatgtctg ctttgtatga
    13141 acgctcacag ctgacgcagg tgatgatttc atctgccccg gcgactgctg aaactatgga
    13201 taaggcggaa tatctgcgcc tggactgcac catcaaggaa gtccagttca ccgccggtca
    13261 gaaacaggat attgatgtga ccacgctctg ctccacagag caggagaaca tcaacggtct
    13321 gggggcgtcg tccgagattt ccatgtcggg taatttttat ctgaatcagg cccagaacgc
    13381 cctgcgtgat gcctatgaca atgacgcgtt gtatgcgttt aaggtgctgt ttccgtccgg
    13441 taagggcttt aagttcctgg cggaagtgcg tcagcacacc tggtcatccg gtaccaacgg
    13501 cgtggtggca gcaacgtttt cactgcgtct gaaaggcaaa ccggtgtcct ttgtggtacc
    13561 gctggcgttt gtgaaaaatc tggataaaac acttaccgtg aataccggtg cgctgctgac
    13621 aatgtcagtc agtgccaacg ggggaacgcc gccgtataaa tacgcctgga agaaggatgg
    13681 tcagccggtt gacgggcaga cgacagacac cttcagtaag ccaggtgcgc agtccgctga
    13741 tgcggggaaa tatacctgcg tggtgaccga ttcggcagag aaagcacaga gtgtgacgtc
    13801 tgttgaatgc accgtgacag tgagcgcagc cgccggataa ggggatgggt catcatgaaa
    13861 aaggatctga aaacgctggc gctggccaga ctgtcagggt ttcgtcataa aacggtgaag
    13921 gtgccggaat ggggtaatgt cagcgtggtg ctgcgggagc cttcggcaga ggcctggtat
    13981 ctgtggcagg aagtgctcaa tggtgatgga gaggatgacg ataccctgtc ggtggtggcg
    14041 aaaacccgcc gtaacctgga agcggatgtg acgctgttct gcgatgtcct gtgtgatacg
    14101 gatctgcagc gggtgttcac tccggacgac cgtgagcagg tgctggccgt ctatggtccg
    14161 gtacatgccc ggttgctgcg tcaggcactg gaactgatcg ctgatgcaga gtcggccaga
    14221 aaaaagtagc ccgcccggaa attcgctttc tgatgcgact tgcgctccgt ctggggcgca
    14281 ccttatccga actgcggcac agcctgagtg cgagcgaggc gatgatgtgg atggagttcg
    14341 acagggtatc cccgctgggt gatgagcgcg gggatatccg taatgcacag atcgtgaaag
    14401 cggtttttgg ggcacagggg atgaatgttg cactgaagga cgccatgctc tgctggggcg
    14461 aggatgagga taagccggag gtggatccgt ttgcggcgct ggaagacgcg ctgagctttg
    14521 cagcacagtc atgaatgatg agaaccgctg aggcggtttt tttacgcccg gagaaaggtg
    14581 aatggcgacg ttacgtgaac tgattatcaa aatttcggca aattcgcagt cattccagtc
    14641 ggagatccag cgggcttccc gcatgggcag tgaatattac cggaccctgc agaatggcgg
    14701 gcgtcaggct gccgcagtcg cccgggagca gcgacgcgcc ctggctgagc tgaacagcca
    14761 gttgacggaa atccgcgctt cagctgccgg aacggcgggg gcatttgcag gtgcctttgc
    14821 caccggacac ctgatttctc tggccgatga atggagttcc gtgaatgccc gactgaaaca
    14881 ggcgtcgcag tcatccgatg aattttcgtc atcacagaaa gtgctgatgg atatcagcca
    14941 gcggacgggc acggcatttt cagataatgc ggccctgttt gcccgctcgg cagcctcaat
    15001 gcgtgaatat ggttacactg ctgatgatgt gctgaaggtg acggaggcca tttccacggg
    15061 gctgaaaatc tccggtgcca gtacggcaga ggcgggctcg gtgatcaccc agttcagcca
    15121 ggcgctggca cagggtgtgt tacgcggtga ggaatttaat tcggtcaatg aaagcggaga
    15181 ccggatcgta cgtgcactgg ctgcgggtat gggcgtggcc cgtaaagacc ttaaggcgat
    15241 ggcggacgac ggcaaactga cggcggataa agtcgttcct gcgttaatca gccagctggg
    15301 ggtattgcgt gatgaatatg ccgccatgcc ggaaacggtc tctgacggga tcacaaaggt
    15361 ggaaaacgcc tttatggcct gggtgggtgg cgcgaatgag gccagtgggg tgacaaaaac
    15421 gctctccggc gtgctgaacg gtgttgccgg taatattgat aatgtggcaa cagcagcggg
    15481 ggcgctggtt gccgtcgggg ttgcccggta ctttggcaat atggcctccg gagcggtgtc
    15541 tgccacggca ggacttgtga cggcagcacg taatgaagtg gcactggcgg aagcacagct
    15601 cagggggacg cagattgcca cggcgcgggc aagggcagcc gtgtaccgtg cacagcaggc
    15661 tgtggcggca gcccgcggga cggagatgca gattgctgca gaagcccgtc tggcggccac
    15721 acaggaacgc ctgaacagaa atattgctgc cagaaccgca gcccagaatg cgctgaacag
    15781 tacaacggcg gtgggctcac gtctgatgac tggtgcgttg ggactggttg gtggcgtacc
    15841 cggactggtg atgctggggg cagcagcatg gtatacgctg taccagaatc aggagcaggc
    15901 cagggagtca gcgcgccagt atgcactgac gatagatgaa atcgcgcata aaacgccgtc
    15961 aatgtctttg cctgaagcct cagataatga aggacgaaca cgggcggcgc tggcagagca
    16021 gaaccggctg attgatgaac aggccagccg ggtgaaatcc ctgcaggaaa aaatcgctgg
    16081 atatcagtat gttctggcta accctggctg gacaaccggt gacggattca tgataaacca
    16141 tctgacatcg gtgaagaccg taacggaagg gctttctcag gcaacagagc agcttgccgt
    16201 tgagcagtcc cgtctggcac agatgcagga aaaagcgcag tccattcagg atgtgcttgc
    16261 cgggctggaa gaccgtcgtg tggcgttaat tcgtcagcag gcggcagagc agaataaggt
    16321 gtaccagtcc atgctggtta tgaacggtca gcatacggaa ttcaaccgtc tgctggggct
    16381 ggggaatgaa ctgcttcagc agcggcaggg actggtgaat gtgccgttac ggctgccaca
    16441 ggccaccctg gatgataaac agcagagtgc cctgacaaaa acagagcgtg agctggccct
    16501 gtcccgactg aagggggagg aaaaagagcg tgcccggctg gggtatgcgg cggatgacct
    16561 tggttttgtg ggggattcgt atcaggaggc gagacagcgt tatatcagta atgctctgga
    16621 agcctggcgt aataacgagg cgaacaaacc taaatcccgg ggtggaaaat cagagacgga
    16681 aaaagcggaa gacagttttt cccggctact gaagcagcag aaagagcaac tggcactggc
    16741 gggtcagaat acagagctgg cgaagctgaa gtaccagact gcgcagggcg aactgaaaac
    16801 cctgacggag atgcagaagc aggaactgct gcgtaacgcg gccctgattg accagcaaaa
    16861 aatccgggaa cagttgcgat cccgggaaga gaccctgaag aatgataatg tggctgcgcg
    16921 tgcatcaaat gaagccgaac tgctggggta cgggcaggga gaacgagcca gggaacgcat
    16981 gcgggagttg cagcagatcc gcgacagctt ccgccagaag gatgcggacc ttcagtctca
    17041 gtatcagacc ggggatatca gtgaggattt ttacagacag gcacgggcac agaacgcgca
    17101 gtatctgagc gaacgcctta aggaccaggc agccttttat gccgaatcgg atgcgcagcg
    17161 tgcggactgg cagaaagggc tgcaggaggg gttcagtaac tgggtggaca atgcgtccga
    17221 ttacgcctca caggcagcac agctggcgac ggagggtatc tcagggatgg tgaataacat
    17281 cacggagatg ctgaacggga ataaagtgga atggcgcaac tgggcctcat ccgtactgca
    17341 ggaaatatca aaagttctta tgaatgccgc gattgtcaac ggaattaaga cggcggcaaa
    17401 cggtatgtcc ggtgcgggag gatttattgg cagcattggt gactggctgg gcggtgcggt
    17461 ggccaatgca aaaggcggcg tgtatacctc ggcaaacctg agcgcgtaca gcaacagtat
    17521 tgtggatacg cccacgtact ttgcctttgc aaaaggggcc gggctgatgg gggaagcagg
    17581 acctgaagcc ataatgcccc tgacccgggc ggcggatggc tcgctgggtg tacgcgcggt
    17641 gggcagtatg aacggcagtg ccggtctggt gtattccccg gtctaccata tcgccattca
    17701 gaatgacggg gccaacggtc agatagggcc ggaggcggca ggcagccttg ttcagctgat
    17761 tgaccagcgg gtgcaggcgg tgatgctgtc catgcgacga gacggaggaa tgctgagtgg
    17821 ctgagataaa aacgctgcat ctggtcccgc gtgaagggat gcaggtgagt gagaaaccgt
    17881 cggtggtgag ggtccggttt ggtgacggtt atgaacagcg ccgaccgacg ggactgaatg
    17941 cccgactgaa gacatttcag gcagtgttcc gggtgacgga tgaggcgacc cggcgctggc
    18001 tggatgaatt tttatcgtgg catggtggtt accgtgcctt tttgtggaaa cccccgaaac
    18061 ataaccggac ggtcagggtg gtgtgccggg agtggagtat tacggataac gccaggtaca
    18121 gcgatttcag ttgtatgatg gaacaggtaa taagataagt ttttgagaaa aaacctaaaa
    18181 gcatcgttta ttaatttgct tgcttgttat ttttgttttg tcttgacata aagtgctatc
    18241 aaagttgttt ttttacattt tgaaacaaaa taaaaattga acccgtaatt taatttacaa
    18301 tattttgagt tttgttcatg acatctctat gcatgaatag ttcagtttaa ataaggatta
    18361 aatcatgaaa aaaatgacag tggcactttc agctgtggca gcagctgtaa tttttgcagc
    18421 gggggcgcag gctgcagaag tatacaataa agatggcaat aaacttgatg tgtatgggcg
    18481 tgcaactgca ctccattatt tctccagtga taagggggat gatggagata agacatacgc
    18541 acgtctgggc tttaaaggtg aaactcagat taatgaccag ttgactggtt ttggtcagtg
    18601 ggaatatcag ttctctggta ataaagctga atcagagggt gctgcgggta ataaaacccg
    18661 tcttgcattt gcaggtctga agtttggtga gtttggcacc attgattatg gtcgtaacta
    18721 tggtattgcg tacgatgttg gttcttatac tgacgtatta ccagagtttg gcggtgatgg
    18781 ctggacccag accgataact tcatgacagc ccgtacttcc ggggtattaa cctatcgtaa
    18841 tacagatttc tttgggcttg tcgatggcct gaattttgcg gcacagtacc agggtaaaaa
    18901 tgatcggggt gatctccgga aagctaatgg tgatggtcac ggtttctcag ccagttatga
    18961 atatgaaggt tttggtatcg ttgccgcata tattaatgcg gaccgaacca ataatcagga
    19021 aaaaggtctg aatggtactc tgacgattga acaaactgat cctttaaccg gtaaagtgac
    19081 tgaaaagcag atcgttgttg attccggtag tgttgcaaaa ggtaaacacg ctgagatgtg
    19141 gggagtcgga ctaaaatacg atgctgacaa cctttacctg gctacaacat attccgaaac
    19201 tcagaatctg acgacctttg gtgataaagg agttgcagat aaagcgcaaa actttgaagc
    19261 ggtactgcag tatcagtttg attttggtct gcgcccgtcc ctggcctacc ttcagtcccg
    19321 cggacaggat gttatggttg gtggtgtgaa ccatggcgat caggatctgg ttaaatatat
    19381 tgatgtcgga gcgacttatt actttaacaa gaatatgtcc acctatgttg attataaaat
    19441 taacctgatt gatgaaagtg aatttacccg gaaagccggt gttgcgacag ataatatcgt
    19501 tgctgtgggt atgacttatc agttctgatt attgctgata agttaataaa tacgggccgt
    19561 ctgcccttac aggcggcctg tatcaatgaa aacacagttt tcattggtca ctgcgatcag
    19621 caattgccat ctggcatgtg ctgatttaac tttctgttat tacctttatt ggttttattt
    19681 taaattggac ttttattgtt cggggcgcgt ctgcgcccct tttttatggg cggata
//
LOCUS       DAZFFE010000089        19709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFFE010000089 DAZFFE010000000
VERSION     DAZFFE010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.089"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(167..448)
                     /gene="yehE"
                     /locus_tag="ACHJ69_003467"
     CDS             complement(167..448)
                     /gene="yehE"
                     /locus_tag="ACHJ69_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO5016381.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVIAADSDSKRRIVLNRYD"
     gene            complement(711..1820)
                     /gene="apbC"
                     /locus_tag="ACHJ69_003468"
     CDS             complement(711..1820)
                     /gene="apbC"
                     /locus_tag="ACHJ69_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO5016382.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1952..3985
                     /gene="metG"
                     /locus_tag="ACHJ69_003469"
     CDS             1952..3985
                     /gene="metG"
                     /locus_tag="ACHJ69_003469"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO5016383.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4126..7905
                     /locus_tag="ACHJ69_003470"
     CDS             4126..7905
                     /locus_tag="ACHJ69_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFO5016384.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTNGQNQVKSFAD
                     AAAAEKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAET
                     RAADKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGAPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKER
                     EDVMDQIIQEGGLEYATDVIIHLQQISIEWDYVNNNIVFLSSGISPDYLQQYSSFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEISQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVTALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAALAALAELLVQK
                     EENSWRIMLMTMLISHPTLAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAIV
                     VSPPWLSKKKKSPIPVLDLAPLNLESICTITDTEAKEFQTHWDWEPHKPSEGAKNFLY
                     SLGYRRWDFDTYKYIGASDSAIDAWEREDFATLIQMFKAHHAPYQGEWHLNSLPFLPM
                     QKAIKLWEFLSKEPHTAIKPVMLYLRLAGMSGFLHSFSRYPQEGFAVANYFAATELAP
                     AVARAFNKLKTLRQDASSCLLKYPEHAITGLLPAAASEAQDNARAALRMLTENGHQPL
                     LQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPTFYQPSLWTRPLLKANAQSLP
                     DSALLHLGEMLRFPQEEALYPGLLQVKDACTTDSLAEFAWDLFTAWQTAGAPSKESWA
                     FTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIAQKL
                     KFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVSFDE
                     TLKPFVRDASGSRLKDLPKPNKSDDESQANDAVNRYKLLKKDARTVAAQQVARLESAM
                     CLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYSTADDDL
                     FTLPEGDISIGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAERNASE
                     LTRWTGRKCPSGRVMGLANKGWIKGTPQDAGWIGWMITPLGRWSLIMEIDEGFAVGMS
                     PAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEALFE"
     gene            7915..11547
                     /gene="yehI"
                     /locus_tag="ACHJ69_003471"
     CDS             7915..11547
                     /gene="yehI"
                     /locus_tag="ACHJ69_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO5016385.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSITARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIVRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCVDKLIAALPGITKVRRPFIALNLPEKPEIANELVSL
                     ECSQTHFRSKEWLKVVADDPKAVKELARYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKAPPARPGYPIIEDKKLPAQQKARDEYWHTLLQTLMASQPQLAEE
                     VMPCLSTQAQAVVNGYLSASPKLAFESTHSNDLPEILVSPPWRGKKKTALLRLDLVPL
                     ELAPKARWQPGERERLAATESARYFSTGSFTERMERKSGRVVLQELGFGDDVWLFRNY
                     ILPGKLDAARKSLVGQWHYSPRRVEEINNGWHSTEAKSAEQALRSGDVEALINTWEND
                     SYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDTLPGLL
                     LAFSHHPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWPALEQLLKLGPIEIYPA
                     RIPKAPDFWHPAMWSRPRLIKNNQPVTDDALEIIGEMLRFTQGGRFYGGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRSLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALIFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLETALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNEQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESASNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGAYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11608..11924
                     /gene="yehK"
                     /locus_tag="ACHJ69_003472"
                     /pseudo
     CDS             11608..11924
                     /gene="yehK"
                     /locus_tag="ACHJ69_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
     gene            12629..13717
                     /gene="yehL"
                     /locus_tag="ACHJ69_003473"
     CDS             12629..13717
                     /gene="yehL"
                     /locus_tag="ACHJ69_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO5016386.1"
                     /translation="MSPQNNHLQRPPATVLYAVELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            13728..16007
                     /gene="yehM"
                     /locus_tag="ACHJ69_003474"
     CDS             13728..16007
                     /gene="yehM"
                     /locus_tag="ACHJ69_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFO5016387.1"
                     /translation="MSEPLIVGIRHHSPACARLVKLLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALARYFA
                     QLRGDYPGDALNRQREAFMARWITWAMQQNNGDVLVVCGGWHAPALAKMWRECPQEIN
                     KPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQCGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVINPDTDPILITLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTGISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGVVHQQGSTLTLAGNGEECWKLTRPLSQHAAL
                     IEAACFGATLQEAARNKLEADMLDAGGIGCITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHTHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPTQSGEALHGLLALARHQLACQPVFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLVQLPVSALQMPLHCPPQDIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            16000..17136
                     /gene="yehP"
                     /locus_tag="ACHJ69_003475"
     CDS             16000..17136
                     /gene="yehP"
                     /locus_tag="ACHJ69_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO5016388.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERYGGLGRSNLTTPEWINSIHTLFPQRVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVHQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFISLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSSATPCYDHDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            17133..19136
                     /gene="yehQ"
                     /locus_tag="ACHJ69_003476"
     CDS             17133..19136
                     /gene="yehQ"
                     /locus_tag="ACHJ69_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO5016389.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTEKKE
                     EEWDPAIWLKELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRS
                     SIRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHATSSDAPFASEE
                     GKTCRQYVQQLSQALWLGGISQPPIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVD
                     AFHARASHYHAGECLRQLAALNSRLNCVQEMARRDSIGEVPPMPWRTVVGAGIAGEAK
                     LDHLRLVSLGMRCWQDIEQYGLRIWFTDPDTGSILHLSRSWQRSEQENSPAATRRLFS
                     FQAGALAGGQIVSQAAKRSADCELLFATRNRLSGVVPLSPDAWLMLSAPLRQPGIVAL
                     REYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNVLTLS
                     LPASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAE
                     TAPVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLPCEQLFPMLQQQG"
     gene            19261..>19709
                     /gene="yehR"
                     /locus_tag="ACHJ69_003477"
     CDS             19261..>19709
                     /gene="yehR"
                     /locus_tag="ACHJ69_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO5016390.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQAISGINVSAEDAKKGITMAQMELVMKAAGF"
BASE COUNT         4992 a         5060 c         4967 g         4690 t
ORIGIN      
        1 attgcaatat aaacacaaca aatacttaag cacattaaat atttatcaaa tctattaatt
       61 aatgtggatt tctataaatg gtattattaa tatcaataaa aaaagcttaa atcattcata
      121 taaatgattt aagctttcta aatccgatta aaaccagggt ggcaaattaa tcgtagcgat
      181 ttaacacgat tcgacgtttg ctatcgctat ctgcagcaat cacttccgtc gttacacctt
      241 taactggcga agaaacctga tgacgggtat caacattata cgttaactga ccacaatgct
      301 gttgaggttg accattaacc atagccatac tgcatgttgg gggactgatc ctaccattaa
      361 tcgttaatgt agcagtttct gaagagaacg cgggagaagc caatccatag gcaagaaaaa
      421 taattcctga cagccagtac ttattcattg aacgttatcc ctgtagtaaa ggttatgcct
      481 ggaaagaatg agtgcataac aaactatagc tgtacatcca ctacacagcc acgaaggatg
      541 ataatgaagc atcgccggta cgatcaatcg actttgtaga gtttcggacg aaggtccgca
      601 gaatattcgc agcattaaat aagtgttcat taatgcggca aaaacaaata caccgcatca
      661 atgtaacatc tctgcggtgt atgaagaata atcagaatgg ttaatacgcg ttagaccgcg
      721 cggaaggaaa tctcgcctgg aatgacttca ccctgccagt agagctgagc tgcaacgcgg
      781 tcagccagct ggcggtagat agcggtaaat tcgctctcag gacggctgat aaccgttggc
      841 gtgcctttat cgagatcctc acgtaaggag atgtgtaacg gcatctgccc cagcagctgc
      901 gtgtggtatt tctcggccag tttctctgca ccaccggtac cgaaaatcgg ttcatggtga
      961 ccacagttac tgcaaatatg cacactcatg ttctcgacaa taccgagcac cggaacttcg
     1021 actttctcga acatcacaat gccttttttc gcatcgatca acgcgatgtc ctgcggggtg
     1081 gtgaccacga ctgcaccggt tactggaatg ttttgtgcca atgtcaattg aatgtcaccg
     1141 gtacctggcg gcatatcaag caccagatag tcgagatccg gccacaaggt ttcctgcaac
     1201 atttgcatta acgccttgct cgccattggg ccacgccaca ccatcgcatt gtcgtctgtc
     1261 accagataac caattgaatt agttgccagg ccatgagaca taatcggtgc catatgggta
     1321 ccgtccggcg aagttggacg ctgattttct gcgcccagca tggtagggat tgatgggccg
     1381 tagatatcgg cgtccagaat gccgactttc gccccttccg ccgccagtgc caacgccagg
     1441 tttaccgccg tggaggattt acccacgcca cctttaccgg aactgaccgc gataatgttc
     1501 ttcacgccgt taattcccgg ctgattcttc acgcgtttca gagtggctat gttatgcgac
     1561 agtttccagt cgatagcttt cgctccggtg atgcgcagca gttcggcgct acattgctct
     1621 tttaactctt cgaacgcgct atgccagacg aatggcataa ccagttccac atgcagcgtg
     1681 tcgtccatcc aggcgacatg gtgcaacgct ttcagcgttg tgaggttatg cttcagggtt
     1741 gggtgctgaa aattggccag tgtcccggcg accattgcgc gcaaggcttc tggtgatttg
     1801 gcctgggatt gttcgttcat cccgactcct tttttgtata gataaaccat cagttgatag
     1861 tttacctgaa gaatatagag aagtacttac ttaacatttt cccatttggt actatctaag
     1921 cccttttcac tattaagaag taatgcctac tatgactcaa gtcgcgaaga aaattctggt
     1981 gacgtgcgca ctgccgtacg ctaacggctc aatccacctc ggccatatgc tggagcacat
     2041 ccaggctgat gtctgggtcc gttaccagcg aatgcgcggc cacgaggtca acttcatctg
     2101 cgccgacgat gcccacggca cgccgatcat gcttaaagct cagcagcttg gtatcactcc
     2161 ggagcagatg attggcgaaa tgagtcagga acatcagact gatttcgcag gctttaacat
     2221 cagctatgac aactatcact cgacgcacag cgaagagaat cgccagttgt cagaacttat
     2281 ctactctcgt ctgaaagaaa acggttttat taaaaaccgc accatctctc agctgtacga
     2341 tccggaaaaa ggcatgttcc tgccggaccg ttttgtaaaa ggcacctgcc cgaaatgtaa
     2401 atccccggat caatacggcg ataactgcga agtctgcggc gcgacctaca gcccgactga
     2461 actgatcgag ccgaaatcgg tggtttctgg tgctaccccg gtaatgcgtg attctgaaca
     2521 cttcttcttt gatctgccct ctttcagcga aatgctgcag gcatggaccc gcagcggtgc
     2581 gttgcaggag caggtggcaa ataaaatgca ggagtggttt gaatctggcc tgcaacagtg
     2641 ggatatctcc cgcgacgccc cttatttcgg ttttgaaatt ccgaacgcgc cgggcaaata
     2701 tttctatgtc tggctggacg caccgattgg ctatatgggc tctttcaaga atctgtgtga
     2761 caagcgcggc gacagcgtaa gcttcgatga atactggaag aaagattcca ctgccgagct
     2821 gtatcacttc atcggtaaag atatcgttta cttccacagc ctgttctggc ctgccatgct
     2881 ggaaggcagc aacttccgca agccgaccaa cctgtttgtt cacggctatg tgacggtgaa
     2941 cggcgcgaaa atgtccaagt ctcgcggcac ctttattaaa gccagcacct ggctgaatca
     3001 ttttgacgcc gacagcctgc gctactacta caccgccaaa ctgtcttcgc gcattgatga
     3061 tatcgatctc aacctggaag atttcgttca gcgcgtgaat gccgatatcg tgaacaaagt
     3121 ggttaacctg gcgtcccgta acgctggctt tatcaataag cgttttgacg gtgtgctggc
     3181 aagcgagctg gctgacccgc agttgtacaa aaccttcact gatgccgctg aagtgattgg
     3241 tgaagcgtgg gaaagccgcg aatttggtaa agctatccgt gaaataatgg cactggctga
     3301 cctggctaac cgctatgtcg atgaacaggc tccgtgggtg gtggcgaaac aggaaggccg
     3361 cgatgccgac ctgcaggcaa tttgctcgat gggcatcaac ctgttccgcg tgctgatgac
     3421 ttacctgaaa ccggtactgc cgaaactgac cgagcgcgca gaagcattcc tcaatacaga
     3481 actgacctgg gatggtatcc agcaaccgct gttgggccat aaagtgaatc cattcaaggc
     3541 tctgtataac cgcatcgata tgaaacaggt tgaagcactc gtggaagcat caaaagagga
     3601 agtaaaagcc gctgccgcgc cagtaactgg cccactggca gatgatccga ttcaggaaac
     3661 catcaccttt gacgactttg ctaaagttga cctgcgcgtg gcgctgattg aaaacgcaga
     3721 gtttgttgaa ggttctgaca aactgctgcg cctgacgctg gatctcggcg gtgaaaaacg
     3781 caatgtcttc tccggcattc gttctgctta cccggatccg caagcactaa ttggtcgtca
     3841 caccattatg gtggctaacc tggcaccacg taaaatgcgc ttcggtatct ccgaaggcat
     3901 ggtaatggct gctggtcctg gtgggaaaga tatcttcctg ctaagcccgg atgccggtgc
     3961 taaaccgggt catcaggtga aataatcccc cttcaaggcg ctgcatcgac agcgcctttt
     4021 ctttataaat tcctaaagtt gttctcttgc gattttgtct ctctctaacc cgcataaata
     4081 ctggtagcat ctgcattcaa ctggataaaa ttacagggat gcagaatgag acactttatc
     4141 tatcaggacg aaaaatcaca taaattctgg gcggtggagc agcaaggcaa cgagttacat
     4201 atcagttggg gcaaagtcgg cactaacggg caaaaccagg taaaaagctt tgctgatgct
     4261 gcggcggcgg aaaaagcgga actgaagctg attgcggaga aggtgaagaa gggatatgtg
     4321 gagcaagcga aggataattc tttgcaacct tcccaaacgg taacggactc tctcaaggta
     4381 gcggacttat ccaccattat tcaggaacaa ccctctttcg tagcagaaac ccgtgcggct
     4441 gacaaaaata cagatgctgt tttaccatgg ctggcgaaag atattgctgt cgtttttccg
     4501 cccgaagttg tacacaccac gttaagtcat cgccgctttc ccggagctcc tgttcagcaa
     4561 gcagacaaat tgacccaatt acgtcgctta gcctgtagtg tatcgcaacg ggataataaa
     4621 acagccacat ttgacttcag cgcctgttct ttagaatggc aaaacaccgt cgcacaggcg
     4681 atcagtcaga tcgacggcct gaaaacaaca cagctaccat cacccgtaat ggctgtactc
     4741 acggcacttg aaatgaaatg cacaagatat aaagagcgtg aggatgttat ggatcagatt
     4801 attcaggaag gcggtctgga atatgctact gatgtaataa tacatcttca acaaatatct
     4861 attgaatggg attatgtcaa caataatatt gttttcctgt cgtctggcat ttcacctgat
     4921 tacttgcagc aatattccag ctttgaatta cgcctacgta aacatttatc actggcggaa
     4981 gagtctctct ggcaaaaatg tgcacaaaaa cttattgccg caattccaca tattccagaa
     5041 tggcggcaac cattaattgc tttgttatta cccgaaaaac cagaaattgc acatgaaatt
     5101 tcccagcgtc tactggggca aaagaaatta ccctcgcttg agtggctaaa aatagtggcg
     5161 actgatgagc acattcttgc ctcattagaa aaatatcacg agccatatgc catttttgat
     5221 gattactatt gtggtgcgat atggtcagcc accgtattac aggagcaagg tgttacagcc
     5281 ctgccccgat ttgctcccta tgccgcaagt gactactgcg ccgatgtgtt gcgtcatatc
     5341 aatcatccgt tcgcattaac actgcttata cgtgtagccg ggcatactaa acgctgtcac
     5401 gatcgaatga cgaaagcctg cgctgcgttc ccacacgcag cactggcagc actggcggaa
     5461 cttctagtgc aaaaagaaga gaatagttgg cgcattatgc taatgacgat gcttatctcg
     5521 catccgacac tagcagaaca ggtcattccc tggctttcga cacccgcagt tgcagtgctg
     5581 aaatcatgtc agcaacaact gacacagccc tcaaaccatg ccagcgccga tctactgccg
     5641 gccatagtag tctccccacc atggctttcg aaaaagaaaa aatcgccgat tccggtgctg
     5701 gatttagcac cgctcaacct cgaatccata tgcacaataa ccgacacaga agctaaagag
     5761 tttcaaactc attgggactg ggaaccgcat aagccaagtg aaggtgctaa aaatttcctt
     5821 tactcgctgg ggtatcgccg ctgggatttt gacacatata aatatatcgg cgcttctgac
     5881 tcggcaattg acgcatggga acgtgaggac ttcgctacac tcatccaaat gtttaaagcc
     5941 caccatgcac cgtatcaggg ggagtggcat ctcaactcac ttcccttttt acctatgcaa
     6001 aaagcgatta agctatggga atttctcagc aaagagccgc ataccgcgat aaaaccagtc
     6061 atgctttatt tgcgactggc aggtatgagt ggttttctac actcgttttc acgctaccct
     6121 caagaaggtt ttgcggttgc taattatttt gccgcaacgg aactggctcc tgccgtcgcc
     6181 cgcgccttca acaaactcaa aacattacga caagacgcca gtagctgtct gctgaaatac
     6241 ccggaacacg ccattaccgg cctgctgcct gcagcggcca gtgaagcaca ggacaacgcc
     6301 cgtgctgcct tgcgtatgct taccgaaaac ggtcatcagc cattgctgca agaaatcgcc
     6361 cggcgttata accagccgga agtaaccgat gcggtgaacg ctctacttgc gctcgatccc
     6421 ttagataatc acccgacaaa aatccccact cttccgacct tttatcagcc atcgctctgg
     6481 acgcgcccgt tattaaaagc aaacgcccaa tcactgcctg atagcgcact cctccacctc
     6541 ggtgaaatgc tccgcttccc tcaggaagag gcactgtatc cgggattatt gcaggtgaaa
     6601 gatgcctgta ccaccgactc actggctgaa tttgcctggg atctgtttac cgcctggcag
     6661 accgctggcg cgccgtcgaa agagagttgg gcgttcactg cgttaggcgt tctcggtaac
     6721 gatgacaccg cccgcaaact gacgcctttg attcgcgcct ggcctggtga atcccagcat
     6781 aaacgcgcca ccgtcgggtt ggatattctc gctgctatcg gtagtgatat cgccctgatg
     6841 cagcttaacg gcatcgccca gaaactgaaa ttcaaagcat tacaggagcg ggcaaaagaa
     6901 aaaattgccg acattgccga gagccgcgaa ctcacggtgg cggagcttga agatcggtta
     6961 gcaccggatc tcggtctgga tgataatggt tcgttgttgc tggatttcgg cccacgccag
     7021 ttcaccgtca gctttgatga aaccttaaaa ccgtttgtgc gtgacgcttc cggcagtcgt
     7081 ctgaaagacc tgcccaaacc aaacaaaagc gatgatgaat cacaggcgaa cgatgctgtt
     7141 aaccgctaca aattgctgaa aaaagatgcg cgtaccgtcg ccgcccagca ggtagcaagg
     7201 ctggaatccg ccatgtgcct gcgtcgccgc tggtcaccgg aaaactttca gctcttcctg
     7261 gttgagcatc cgctggtccg ccacttaacc cgccgtctga tttggggcgt ttatagcgcc
     7321 gaaaaccagc tactggcttg ctttcgcgta gcggaagata acagctacag caccgctgac
     7381 gatgatcttt tcaccctgcc ggaaggcgat atctctattg gtattcctca cgttctggaa
     7441 atatcaccga cggatgctgc cgccttcggg cagctttttg ccgactacga actgttacca
     7501 ccgttccgcc agcttgatcg taacagttac gcgctgacag aagccgagcg caacgccagc
     7561 gaactgacac gctggacagg cagaaaatgc ccgagtggtc gggtaatggg gctggcgaat
     7621 aaaggctgga taaagggcac cccgcaggat gctggctgga tcggctggat gatcaccccc
     7681 ctgggccgct ggtcgttaat catggaaatc gatgaaggtt ttgcggtagg catgtcgcca
     7741 gccgaactga gtgccgagca actcttaagc aagctgtggc tatgggaagg caaagcggaa
     7801 agctatggct gggggagtaa ttcaacacag gaagcgcagt tctccgtact cgatgccatc
     7861 accgccagcg agctaattaa cgatattgaa gccctgtttg aataaggaaa gagcatggac
     7921 aaggaattac cgtggctggc ggataacgcc caactggaac ttaaatataa aaaaggcaaa
     7981 acaccactca gccatcgcaa ttggccaggc gaaccggtgc ctgttatcac tgaaagcatc
     8041 attcagacac tgggtgatga attgctacaa aaagcagaga agaaaaaaaa cattgtctgg
     8101 cgttatgaaa acttttcact ggagtggcag tccgccatca cgcaggccat caacttgatc
     8161 ggcgaacaca aaccctcaat cacggcccgg acaatggcgg cgctagcctg tatcgcgcaa
     8221 aatgatagcc aacagttgct cgacgaaatc gtccaacaag aggggctgga atatgcaact
     8281 gaggtggtga ttgcacgcca gtttattgta cggtgttatg agagtgatcc tctggtagta
     8341 acattgcaat atcagaacga ggactatggc tatggttatc gctcagaaac ctataacgaa
     8401 ttcgatctcc gattgcgtaa gcatctctct ctggcagagg aaagctgctg gcagcgttgc
     8461 gtcgacaaac tcattgccgc actgccagga ataaccaaag ttcgccgccc ttttattgcg
     8521 ctgaacctcc cggaaaaacc cgaaatcgcc aacgagctgg taagccttga atgttcgcaa
     8581 actcattttc gctcaaaaga gtggttaaaa gttgttgctg atgaccctaa ggcggtgaaa
     8641 gaactcgcac gctactggag ccaggatata tttagcgatc gagaagccag ctacatgtcg
     8701 catgaaaacc acttcggtta cgcggcctgc gctgcccttt tgcgcgaaca aggactggca
     8761 gccgttccgc gcctcgcgat gtatgcacat aaagaagatt gcggcagtct gctggtacaa
     8821 attaaccatc cgcaagtcat ccgcaccttg ctactggtgg ctgataaaaa caaacccagc
     8881 ctgcaacgtg tggctaaata cagtaaaaac ttcccccatg cgacgctcgc cgcactggca
     8941 gaactgctgg cgttaaaagc cccccctgcg cgccctggtt atcccatcat cgaagacaaa
     9001 aagctgcctg cacagcaaaa agcacgtgat gaatactggc atacgctgtt acaaacgctg
     9061 atggcatcgc agccacaact ggcagaagag gtgatgccgt gcttaagtac acaagcccag
     9121 gcagtggtga atggttattt atcggcatcg ccaaaacttg ctttcgagag tactcatagc
     9181 aacgacctgc cagaaatact tgtttctcca ccgtggcgag gtaagaaaaa aacggcattg
     9241 ctgcgccttg atttagtgcc tcttgaatta gcgcccaaag cccgctggca accaggcgaa
     9301 cgagagagac ttgccgccac agagtctgcc cgttatttca gcacgggatc ttttacggaa
     9361 cgcatggaac gaaaaagtgg tcgagttgta ctacaggagc taggttttgg ggatgatgta
     9421 tggctgttcc ggaattatat actgcccggc aaactggatg cggcgcgcaa atcattagtg
     9481 ggtcagtggc attactcccc gcgcagagta gaggaaatca acaatggctg gcactccacg
     9541 gaagcaaaat cagccgaaca ggcgcttcgt tccggcgacg ttgaagcgtt aattaacacc
     9601 tgggaaaatg atagctactc acgttatcgt ccggaaaaga gtgtctggaa cctgtattta
     9661 ttggcacagt tgccgcgtga gatggctttg actttctggc tgcgtatcaa tgagaaaaag
     9721 catctgttcg cgggtgagga ctattttctc agtatcctcg ggttggatac attacctggc
     9781 ctgttattgg ctttttcaca tcatccaaaa gaaacatttc cgttaatttt aaatttcggc
     9841 gcaacagaac tggccctgcc cgttgcccgc gtctggcgac gtttcgcggc gcagcgtgat
     9901 ctggctcgcc agtggatttt acaatggccg gaacatacgg ctactgcact tatccctctt
     9961 gtctttacaa aacccagcga taatagcgaa gctgcattac ttgccctgcg tttactgtat
    10021 gaacaagggc atggcgaatt actgcaaacc gtggcaaacc gctggcagcg tacagatgta
    10081 tggcctgccc tggagcaatt acttaaactg ggtccaatag aaatttaccc ggcacgtatt
    10141 ccaaaagctc ctgatttctg gcatccggca atgtggtcca ggccgcgcct tatcaagaat
    10201 aatcagcctg ttaccgatga cgctctggaa attatcggcg aaatgctgcg ctttacccag
    10261 ggcggacgtt tttatggcgg actggaacag ctgaaaacgt tttgccagcc gcaaacgctg
    10321 gcagcttttg cctgggatct tttcaccgca tggcaacaag ctggagcccc cgcaaaagac
    10381 aactgggcat ttctggcgtt aagtctcttt ggtgacgaaa gcacggctcg ggatctgacg
    10441 acacagatcc tcgcctggcc acaagaaggc aaatctgccc gtgctgtcag tggcctgaac
    10501 atccttaccc tgatgaataa tgatatggcg ctgatacagc tgcaccatat atcccaacgg
    10561 gcgaaatccc gctccttacg tgataacgcg gcggaatttc ttcaggtggt cgcagaaaat
    10621 cgcgggctaa gccaggaaga gttagcggac agattagtcc caacactggg tcttgatgat
    10681 ccgcaggcgt tgatttttga ttttggtcca cggcaattta ccgttcgctt cgatgaaaac
    10741 ctcaacccgg ttatctttga tcagcaaaac gttcgccaga aaagcgttcc ccggttgcgc
    10801 gccgatgacg atcaactgaa agcccccgag gcactggccc gactcaaagg gctaaaaaaa
    10861 gatgcgactc aggtgagcaa aaacctgctc ccgcgtcttg aaactgccct gcgcaccacc
    10921 cgacgctggt cgctggcaga ttttcattct ctgtttgtta atcatccctt tacccgtctg
    10981 gttactcagc gattaatatg gggggtttat ccggcaaatg aaccgcgtcg tttacttaac
    11041 gcctttcgtg tggccgctga gggggagttc tgcaatgagc aagatgagcc aattgacctg
    11101 cctgccgatg ctctgattgg cattgcccac ccgttagaaa tgacagcaga aatgcgcagt
    11161 gaatttgcac agctttttgc cgattacgaa attatgccgc cttttcgcca gttgtcgcgc
    11221 cgcacggtgc tgctcacacc tgacgagtca gccagtaaca gcctgactcg ctgggaaggt
    11281 aaatccgcta ccgttggtca acttatggga atgcgataca aaggctggga gtcaggctat
    11341 gaggacgcat ttgtctatga cttaggtgcg taccggctgg tccttaagtt ttcacccggt
    11401 tttaaccact acaatgttga tagcaaagcg ctaatgagct tccgttctct tcgggtttac
    11461 cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt ttgatttgag tgaggcatta
    11521 agcgcacctg acgtcatttt ccattaaaac acagcgggca gtgatgcaac tgcccgttat
    11581 caacacgacc taccagcggg ggaagcgatg atcgtgcaaa aagagctggt tgctatttac
    11641 gattatgaga tccctgtacc tgaagagccg ttttccttca gacttgaaat ccataaatgc
    11701 tctgaattat ttacaggttc cgtctatcga ctggagcgat tccggctaca tccaacattt
    11761 catcaacgtg atcgagaaga tgccgaccac taataaatga tgcgtttatt tatataagag
    11821 atgagtttat tgatgagcgg aaattacgag gtgaatcaca tgaaacagta atagcaattt
    11881 ttaattgtga actacagaat atattcaacc aagaaataga ataatatact ctagataatt
    11941 caagtcgcag gaaggcgata agcgagtgaa tccccaggag cttactaaag taagtgacag
    12001 gggtgaacga acgcagtcgc agcacatgca acttgaagta tggcgagtaa agctttatta
    12061 aagggggaaa cggtattccc ccttagatgc gtttcaattt gcccgttact tcaacaaatc
    12121 aatcaaccgt aacagagcca ctaatagctc cagtacagca atgagcacgg ttaacttttg
    12181 tgtcagcgtg tccatccgct ctccttatta aggagcgcaa caccagcacc ttacccttac
    12241 actgcctgtc gatactggtt tacgtatgtt ggaccacgat gttgcactcc ggatcagggc
    12301 accacctgca gcaccacccg tccggtagcc aggacttgaa attggttcta tccggcgcaa
    12361 cctcccaaca gtcaggatta taaatctcat tatcttaatt gcaacggggt ccagcggtgg
    12421 ttataattcg ttcgttggaa cacgatgttg cacaggctgc ggtgttaggc ctgaaagtag
    12481 taaataaaag aacctgcctc accagcaggt ttttttattt tttgcgatct gctttccaga
    12541 tattttccgc atcaaacaac taatgcgcca aacagttatt gcgcgtaaaa tatcgtttat
    12601 ttcattaata catttcaggg atgaatatat gtcaccgcag aataatcacc tgcagcgtcc
    12661 gcctgctact gtgttatacg ccgttgaact ggcaaaatta aaacaaaatg ataacgcacc
    12721 ttgcccgcca ggttggcagt taagtttgcc tgcggcccgt gcttttatcc ttggcgatag
    12781 cgcgcaaaat attagccgta aagtggtgat tagtccctcc gctgtcgaac gtatgttagt
    12841 cactcttgcc accgggcgcg gtttgatgtt ggtgggggaa cctggcaccg caaaatctct
    12901 cctgtctgaa ttactggcaa ccgctattag cggcgatgcc gggttaacca tccagggcgg
    12961 cgcatctact actgaagatc aaatcaagta cggctggaac tacgccctgc ttatcaacca
    13021 tggtccgtca acggaagcac tggtccctgc cccgctttac cagggaatgc gcgacggcaa
    13081 aatcgtccgt tttgaagaga taacgcgtac accgctggaa gtacaagact gtctgttagg
    13141 tatgctttct gacagggtga tgacggtgcc ggaactcacc ggtgaagcca gtcagctcta
    13201 tgcgcgggaa ggctttaata ttattgccac tgccaatacc cgcgatcgcg gcgtcaatga
    13261 aatgagtgcc gcgctaaagc gtcgcttcga ttttgaaact gtctttccga ttatggattt
    13321 cgcccaggag ctggagcttg tcgccagtgc ttcggcgcgt ttgctggccc atagcggcat
    13381 tccgcataaa gtgcctgacg cggtactgga gttactggtc cgcacctttc gcgatctgcg
    13441 tgccaacggc gaaaagaaaa cgtcaatgga tacactgaca gcgattatgt ccaccgctga
    13501 agccgtgaac gtggcccacg cggtgggcgt ccgcgcctgg tttttagcga atcgcgcagg
    13561 tgagccagcc gatctggtgg actgtattgc cggaaccatc gtcaaagata acgaagaaga
    13621 tcgcgcgcgc ctgcgccgtt acttcgaaca acgtgttgct acacataaag aagctcactg
    13681 gcaggcttat tatcaagctc gccaccgcct gccgtgagga aagatgtatg agcgagccgt
    13741 taattgtcgg catccggcat catagcccgg cctgcgcccg gctggtgaaa ttgttaatcg
    13801 aaagccagcg gccacgatac gtattgattg aaggcccggc tgattttaat gaccgggtgg
    13861 acgaactgtt tttagcccac cagcttccgg tagctattta cagttattgc cagtatcagg
    13921 acggtgcggc ccccgggcgt ggtgcctgga cgccatttgc tgaattttcg ccggagtggc
    13981 aggcgctaca agccgcacgt cgtattcagg cacaaactta cttcatcgat ttgccttgct
    14041 gggcgcagag tgaagaagag gacgattcgc ctgatacaca agatgaaagc cagaccttac
    14101 tgctgcgtgc cacccgcatg gataacagcg ataccctgtg ggatcactta ttcgaagatg
    14161 aaagccagca aactgcatta ccctctgcac tggcgcgcta ttttgcccaa ctgcggggcg
    14221 attaccccgg agatgctctc aatcgtcagc gcgaagcctt tatggctcgc tggattacat
    14281 gggcgatgca gcaaaacaat ggcgacgtgt tagtcgtctg tggcggctgg cacgctcctg
    14341 cactggcaaa aatgtggcgc gaatgcccgc aggaaattaa caagccagaa ttgccctcgc
    14401 tggcagatgc cgttactggt tgttatctca caccctacag tgaaaagcgc cttgatgtgc
    14461 tggcaggata cctttccgga atgcctgccc cggtctggca aaactggtgc tggcagtgtg
    14521 gcttacagca ggcaggtgaa cagctgctga aaacggttct tacccgtttg cgccagcaca
    14581 aattgcccgc ttctaccgct gatatggctg ctgctcatct gcatgctatg gcactggcac
    14641 agttgcgcgg ccatacacta cctctacgca ctgactggct ggatgccata gctggttcgc
    14701 tgattaaaga agccttgaac gcgccgttgc cgtggagcta ccgcggagtc attaatcccg
    14761 ataccgatcc gattctgata acgttgatag acacattagc gggtgacgga ttcggtaaac
    14821 ttgccccttc tacgccacaa ccgcctctgc caaaagatgt cacctgcgaa ctggaacgta
    14881 ccggaatctc tcttccggcg gagcttacct taaatcgctt tacccccgat ggactggcgc
    14941 aaagtcaggt gttacatcgg ttggcaatac tggagatccc aggggttgtg caccagcagg
    15001 gaagtacact gacacttgca ggcaacggtg aagaatgctg gaaattaacc cgcccgctta
    15061 gccaacatgc ggcattaatt gaggccgcct gctttggtgc cacactccag gaagccgcac
    15121 gcaataaatt agaagccgat atgctggacg cgggtggaat cggctgtatc accacatgtc
    15181 ttagccaggc agcgttagcg ggtctggcgt ctttcagtca gcaattactg gaacaactca
    15241 cattattaat cgcccaggaa aatcaattcg ccgaaatggg ccaggcgctg gaagtgctat
    15301 atgccttatg gcggctggat gaaattagcg gtatgcaagg cgcgcagata ttacagacga
    15361 cgttatgcgc ggctatcgat cgcacactct ggctatgtga atccaacggc agaccggatg
    15421 aaaaggagtt tcacactcac ctgcatagct ggcaggcgct ttgccatatc ctgcgcgatc
    15481 tacatagcgg cgttaattta cccggcgttt cgctttctgc ggcggtagcc ttactggagc
    15541 gacacagtca ggcgattcat gtcctggcac tggatcgcgg cgcgactctt ggtgcgctga
    15601 tgcgtctgga acatcccaac gccagtgccg aagcggcgct gacgatgctg gcgcagttat
    15661 ccccgacaca atccggcgag gcgctgcacg gtttgctggc attagcccgt catcaactgg
    15721 cctgtcagcc ggtatttatc gccggtttca gcagtcattt aaatcaactg agtgatgccg
    15781 attttatcaa tgccctgccc gatttacgcg cggcgatggc ctggctacca ccacgagaac
    15841 gcgggacgct ggcgcatcag gtgcttgagc attatcaact ggtgcaactt cccgtttcgg
    15901 cactgcaaat gccgttgcat tgtccaccac aagacattgc acatcatcaa caactcgaac
    15961 agcaggcact ggcatcgctg caacactggg gagttttcca tgtctgaact gaacgatctt
    16021 ctgaccaccc gtgagctaca acgctggcga ttaattcttg gcgaagcggc agaaacgacg
    16081 ctttgtgggc tggatgacaa cgcccggcag atagaccacg cgctggagtg gctgtatggg
    16141 cgcgatcctg aacggctcca gcgtggtgaa cgttacggtg gattaggtcg ttcaaatctc
    16201 accacccctg agtggatcaa cagcattcac acgctgtttc cgcaacgggt gatcgagcgg
    16261 ctggaaagcg atgccgtgct gcgctacggc attgaagatg tggtgacgaa tctcgacgtg
    16321 ctggaacgta tgcagccttc tgaaagcctg ctacgcgccg ttttgcacac caaacatctg
    16381 atgaatcccg aagtactggc tgccgcccgc cggatagtgc accaggttgt tgaagagatt
    16441 atggcgcgac tggcaaagga agttcgtcag gctttttctg gagtacgcga tcgccgtcgc
    16501 cgctcattta tttcactggc gcgaaacttt gatttcaaaa gtactctgcg cgccaacctg
    16561 caacactggc acccgcaaca cggcaagttg tatatcgaat ccccccgctt taacagccgc
    16621 attaaacgcc atagcgaaca atggcaactg gtcctactgg ttgatcaaag cggatcgatg
    16681 gttgattcgg tgatccactc cgcagtgatg gcggcttgtt tatggcagtt acccggcatt
    16741 cgtacccatc tggtcgcttt tgacaccagc gtggttgatc tcacggcaga cgttgccgat
    16801 ccggtagagc tattaatgaa agtgcaatta ggcggcggga ccaatatcgc cagtgctgtg
    16861 gagtatggtc ggcaactgat tgaacaacca gcgaaaagcg tcattatcct cgtgagcgat
    16921 ttttacgaag gggggtcatc atcattgctg acgcatcagg tgaaaaagtg tgtccagagc
    16981 ggcatcaaag tgctgggact ggcggcgctc gatagcagcg caacaccttg ctatgaccac
    17041 gatacggccc aggcgctggt taatgtcggc gcacaaatag ccgcaatgac accgggcgag
    17101 ctggcttcct ggcttgcgga gaatcttcag tcatgaattc actacgtccg gaattattag
    17161 aactgacacc acaggcactg acggcgttaa gcaatgccgg ttttgttaag cgcagcctta
    17221 aggaactgga aaatggcaac gtcccggaaa tcagtcatga gaacggcgct ttaatcgcca
    17281 ccttcagtga cggtgtccgt acccagctgg cgaacggcca ggcactgaaa gaggctcagt
    17341 gcacctgcgg ggccagcggc atgtgccgtc atcgcgtgat gctggtgtta agttatcaac
    17401 gactttgtgc caccgcccag cctacggaaa aaaaagaaga agagtgggat ccggcaatct
    17461 ggctgaaaga actggctacc ttgcccgatg ccacccgcaa acgcgcgcag gcgctggtcg
    17521 ctaaaggcat caccattgag ttgttctgtg cgcctggcga aattccctct gcccgattac
    17581 cgatgagcga tgtgcgtttt tattcccgca gcagcattcg cttcgcacgc tgtgattgca
    17641 ttgaaggcac actttgcgaa catgtcgtac tggctgtgca ggccttcgtc gaggccaaaa
    17701 ctcagcaagc ggaatttact catttaatct ggcagatgcg cagtgaacac gccacatcat
    17761 ctgatgctcc gtttgccagc gaagaaggca aaacgtgtcg tcaatatgtt cagcaattaa
    17821 gccaggcatt atggctgggc gggataagtc aaccgccaat ccattacgag gccgctttca
    17881 gtcgcgcgca gcaagcggcg gaacgctgca actggcgatg ggtgagtgaa tcgctacggc
    17941 aattgcgcgc cagtgttgat gccttccacg cccgtgccag ccactaccat gccggagaat
    18001 gcttacgtca gcttgcggca ttaaacagtc gattaaattg cgtacaagag atggcgcgac
    18061 gcgacagtat cggtgaagtt cctcccatgc cgtggcgcac ggtcgttggc gctggtattg
    18121 ccggagaagc aaaacttgat catctgcggc tggtgtcttt aggtatgcgt tgctggcagg
    18181 atattgagca gtatggttta cgcatctggt ttaccgatcc cgacacagga agtattttgc
    18241 atctttcgcg tagttggcag agaagcgaac aggaaaactc accggcagcg acgcgtcggc
    18301 tgtttagttt tcaggctggc gcactggcag gtgggcaaat tgtttcacaa gctgcaaaac
    18361 gcagtgccga ttgcgagctg ctgttcgcga cccgtaaccg cttgagtggc gttgttccac
    18421 tgtcgccaga cgcctggcta atgttgagcg cgccgttgcg ccagccgggc attgtggctt
    18481 tgcgggaata tttacgccag cgtccccccg cctgcatacg accactcaac caggtcgata
    18541 acttatttat tctgccggta gcggagtgta tttcgctcgg ttgggatagc agccgccaga
    18601 cactggatgc gcaggtaatc agcggcgaag gggaagataa tgtgctgacg ttatcccttc
    18661 cggcatctgc ctgttctcct tttgccgttg aacgcatggc ggcgcttttg caacaaacag
    18721 acgaccccgt gagcctggtt tctggctttg tcagttttgt agaggggcaa ttaacactgg
    18781 aaccacgggt gatgatgaca aaaacccgcg cctgggcgct ggacgcagaa actgcgcctg
    18841 tggcaccgct accttctgcc agcgttctgc ctgtgccgtc taccgctcat cagttgctga
    18901 tgcgctgcca ggcgttactt attcaactgc tccataacgg ctggcgctat caggaacaga
    18961 gtgctattgg tcaggcagag ttgctggcga atgacctcac cgcggtgggt ttttatcggc
    19021 tggcacatgt gttgggacaa tttcgtaata cagaaagcga ggcacgggta gaagcaatga
    19081 ataacggtgt tttgccttgc gaacaattat tccccatgct tcagcaacaa ggatgaaata
    19141 gtgcttttta ttaagagttc tactcctgtt ccggactgct cacgccacag tattaggcat
    19201 atcctatata gcccctggtg agagtcacca gttccttgat taaataaaat ggagttttac
    19261 atgaaggctt tcaataagct gttttccctt gttgttgcat ctgttctggt tttctctctt
    19321 gctggctgcg gtgacaaaga agaatcaaag aaattcagcg ccaatctgaa cggcactgaa
    19381 attgccatta cctatgtcta caaaggtgac aaggtgctta agcaatcttc tgaaaccaaa
    19441 attcaatttg cctccattgg tgcaaccacc aaagaagatg ccgccaggac acttgagccg
    19501 ttaagcgcta aatacaaaaa catcgcgggt gttgaagaaa aattaaccta taccgatacc
    19561 tacgcgcagg aaaacgtgac tattgatatg gaaaaagtgg attttaaagc cctgcaggct
    19621 atttcaggaa tcaacgtttc tgctgaagat gctaaaaaag gtatcactat ggcgcaaatg
    19681 gaactggtga tgaaagccgc tggttttaa
//
LOCUS       DAZFFE010000090        19659 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFFE010000090 DAZFFE010000000
VERSION     DAZFFE010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.090"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            129..671
                     /gene="yfaZ"
                     /locus_tag="ACHJ69_003478"
     CDS             129..671
                     /gene="yfaZ"
                     /locus_tag="ACHJ69_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO5016391.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDAAGVGLGLNLPLGPLMATVGGKGVYTNPNDGDEGYAAAVGGG
                     LQWQIGDSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLAGK
                     NGNRDNAIADGPYVGVNASF"
     gene            771..1973
                     /gene="yfaY"
                     /locus_tag="ACHJ69_003479"
     CDS             771..1973
                     /gene="yfaY"
                     /locus_tag="ACHJ69_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO5016392.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAIQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEEQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQSRQFSLTLSEQF
                     TGGLLALQLSRADAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGLEDEH
                     LNFALATPDGTFALRVHFSATRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2193..2975
                     /gene="yfaX"
                     /locus_tag="ACHJ69_003480"
     CDS             2193..2975
                     /gene="yfaX"
                     /locus_tag="ACHJ69_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO5016393.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPEAVQKSIIEEL
                     VWEQMTPTTITHPQRLYEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            2990..4195
                     /gene="rhmD"
                     /locus_tag="ACHJ69_003481"
     CDS             2990..4195
                     /gene="rhmD"
                     /locus_tag="ACHJ69_003481"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFO5016394.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYAIKLAHACAPYNLKWIEECLPPQQYEGYRELKHNAPAGMMVTSGE
                     HHGTLQSFRTLSENGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4252..5541
                     /gene="yfaV"
                     /locus_tag="ACHJ69_003482"
     CDS             4252..5541
                     /gene="yfaV"
                     /locus_tag="ACHJ69_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5016395.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFINLFGAVGGFIAPILRVKAETLFS
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5559..6362
                     /gene="yfaU"
                     /locus_tag="ACHJ69_003483"
     CDS             5559..6362
                     /gene="yfaU"
                     /locus_tag="ACHJ69_003483"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFO5016396.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASHPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMVQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRVKGSY"
     gene            complement(6403..6600)
                     /locus_tag="ACHJ69_003484"
                     /pseudo
     CDS             complement(6403..6600)
                     /locus_tag="ACHJ69_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6601..7560)
                     /gene="rpnE"
                     /locus_tag="ACHJ69_003485"
     CDS             complement(6601..7560)
                     /gene="rpnE"
                     /locus_tag="ACHJ69_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO5016397.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLWQEGHQIGWQEGKIEGWQEGKLEGLQ
                     KGKVEGMHEQAIKIALRMLEQGFEREIVLATTQLTDADIPNCY"
     gene            complement(7753..8943)
                     /gene="glpC"
                     /locus_tag="ACHJ69_003486"
     CDS             complement(7753..8943)
                     /gene="glpC"
                     /locus_tag="ACHJ69_003486"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO5016398.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSLCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(8940..10199)
                     /gene="glpB"
                     /locus_tag="ACHJ69_003487"
     CDS             complement(8940..10199)
                     /gene="glpB"
                     /locus_tag="ACHJ69_003487"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO5016399.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTEIHSGLESLRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKTCVVGISGLMDFQAHLAAASLR
                     ELDLKVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWHWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQLFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHVAQQIAQRAGGQQ"
     gene            complement(10189..11817)
                     /gene="glpA"
                     /locus_tag="ACHJ69_003488"
     CDS             complement(10189..11817)
                     /gene="glpA"
                     /locus_tag="ACHJ69_003488"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO5016400.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEVIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGEIQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEATLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12090..13448
                     /gene="glpT"
                     /locus_tag="ACHJ69_003489"
     CDS             12090..13448
                     /gene="glpT"
                     /locus_tag="ACHJ69_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO5016401.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13453..14529
                     /gene="glpQ"
                     /locus_tag="ACHJ69_003490"
     CDS             13453..14529
                     /gene="glpQ"
                     /locus_tag="ACHJ69_003490"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO5016402.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNVLEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            14732..15382
                     /gene="inaA"
                     /locus_tag="ACHJ69_003491"
     CDS             14732..15382
                     /gene="inaA"
                     /locus_tag="ACHJ69_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO5016403.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(15436..15690)
                     /gene="yfaE"
                     /locus_tag="ACHJ69_003492"
     CDS             complement(15436..15690)
                     /gene="yfaE"
                     /locus_tag="ACHJ69_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO5016404.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(15690..16820)
                     /gene="nrdB"
                     /locus_tag="ACHJ69_003493"
     CDS             complement(15690..16820)
                     /gene="nrdB"
                     /locus_tag="ACHJ69_003493"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO5016405.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(17010..19295)
                     /gene="nrdA"
                     /locus_tag="ACHJ69_003494"
     CDS             complement(17010..19295)
                     /gene="nrdA"
                     /locus_tag="ACHJ69_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO5016406.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4529 a         4872 c         5338 g         4920 t
ORIGIN      
        1 atcaccaaga agtatttagt tacaataagt gactagatcc cttttttcgc tcattgaatc
       61 ctctgcgcca ggcagtacaa tcccgcagct ttgggaattt tgattaattg cttgtgggaa
      121 agaggataat gaaaaaaatt gcgctagcag gtctggccgg aatgctgttg gtttctgcat
      181 cggtcaatgc aatgagcatc agcggccagg cgggtaaaga atacaccaat attggtgtcg
      241 gttttgggac tgaatcgacg ggcctggcgt taagcggtaa ctggacacat aacgacgacg
      301 acggtgatgc cgcgggcgtg gggctggggt tgaatctgcc tctcgggcca ttaatggcga
      361 ccgttggcgg aaaaggcgtt tacaccaacc cgaacgatgg tgatgaaggt tatgcagcgg
      421 cagttggcgg tggtttgcag tggcaaattg gtgacagctt ccgcttgttt ggtgagtatt
      481 actactctcc ggattcgctc tccagcggaa ttaaaagtta tgaagaagcg aatgctggcg
      541 cgcgttacac cattatgcgt ccagtcagca ttgaggctgg ctaccgttac ttgaacctgg
      601 caggtaaaaa tggcaatcgt gataacgcca ttgctgatgg tccgtatgtt ggggtaaatg
      661 ccagcttctg acgccttccg gcgcggcgtt ctgtcgcgcc attcagtttc ttactacgcc
      721 ctgtctccct ttgcgttata gtgattcgat aacaaaagca ggagataacc atgttaaaag
      781 tggaaatgtt atccaccggg gatgaagtgt tacacgggca aatcgttgac actaacgctg
      841 cctggctggc cgattttttc tttcatcagg ggctgccatt atctcgccgc aatacggtgg
      901 gggataacct tgatgactta gtcaccattc ttcgcgaacg tagtcagcac gccgatgtgt
      961 tgatcgttaa cggcgggctg ggaccgacca gtgatgattt aagcgcactc gccgctgcga
     1021 cagcaaaagg tgaaggcctg gtgctgcatg aagcctggct caaagagatg gaacgctatt
     1081 tccatgaacg gggacgagta atggcaccga gcaaccgtaa acaagcggag ctgcctgcca
     1141 gtgctgaatt tatcaataac ccggtaggca ctgcctgtgg ttttgccata cagcttaatc
     1201 gttgcctgat gttctttacc cccggcgtac cgtcagaatt taaggtgatg gtcgagcacg
     1261 aaatcctgcc gcgcctgcgc gagcgttttt ctttaccaca gccgccggtt tgcctgcgtt
     1321 tgactacctt tggtcgttcg gaaagcgatc tggcacaaag cctggacact ctacaactgc
     1381 cgccgggcgt aacaatgggc tatcgctcct caatgccgat tatcgaactg aaactcaccg
     1441 gaccggcaag cgaggaacag gcgatggaaa aactgtggct ggacgttaaa cgtgttgccg
     1501 gacagagcgt gattttcgaa ggcaccgaag gactgcccgc gcagatcagt cgcgaattgc
     1561 agagccgcca gttcagcctg acgctgagcg agcaattcac cggtggttta ttggctttgc
     1621 aactttctcg cgcagatgct ccattgctgg cgtgtgaagt ggttccttca caggaggaaa
     1681 ccctggcgca aactgcgcac tggattacag aacggcgggc gaaccatttt gctgggttgg
     1741 cactggctgt ttctggtctg gaagatgagc atctcaactt tgcactagcc acgccagacg
     1801 gcactttcgc tttgcgtgta catttcagtg ccacacgcta cagtctggct attcgccagg
     1861 aagtctgcgc aatgatggcg cttaatatgc tacgccgttg gttaaacggc caggacatcg
     1921 ccagcgagca tggctggatt gaggttgttg agtccatgac cttatctgtc tgattccgcc
     1981 actctgtgcc ggtttactgc accggcacat tccccctccg gcaaaatttg tccaacctgt
     2041 tgtactcaat aagaaaaaat ccgtgcgctg attcacaaat ttgtgcgtaa tttctccagt
     2101 ccgctggacg agaaacggga tttgatgcgc ttcacaattt tactccccct gagcaacgag
     2161 actgattcca gtatctaaga ctggagtcca gtatgttgga atcaagcaaa gttcccgccc
     2221 tgacacgggc tattgatatt ctcaatttga ttgcccggat tggcccctgt agtgctgcga
     2281 cgatcattga cacattgggg atccctaaaa gcacagccta tttgctgctt aatgaactca
     2341 ggcgtcagcg ttttcttagc cttgatcacc aggaaaattt ttgtttgtgg accaggctgg
     2401 tggagctttc cggtcatgcg ttaagcaaga tggatctccg ggaactggct cgaccgcgtt
     2461 tgacgcaact gatggatacc accgggctgt tatgtcacct ggggattatt gataacggca
     2521 gcgcttatta cattttgaaa gtggagtcat cagccactat cagcgtgcgt tcccatgaag
     2581 gaaaaagcct ttcgctttat cgctccggaa taggtaaatg cctgcttgcc tggcaacctg
     2641 aagctgtaca gaaaagtatt atcgaagagc tggtctggga gcagatgaca cccaccacca
     2701 ttacccatcc acaacggctc tacgaggaac tggcgcgtat tcgccgtcag ggctggagct
     2761 atgacaacgg cgaagattat gccgatgtcc gttgtgtcgc tgcgccggtg tttaacgcca
     2821 ataacgagct taccgctgcg atttcggtgg tcggtacccg cttacaaatc aatgaagaat
     2881 accgtgatta tctggcgggc aaggccattg cctgcgccag ggatatttca cgtttgttgg
     2941 gatggaaaag tccctttgac ttacaggcct cataacaatg gagaacatca tgaccctacc
     3001 aaaaatcaaa caggttcgcg cctggtttac tggcggtgcg acagcagaaa aaggcgctgg
     3061 cggcggtgat tatcacgacc agggggcgaa tcactggatt gatgatcata ttgccacccc
     3121 gatgagtaaa taccgcgatt acgagcagtc acgccagtca tttggcatta acgttcttgg
     3181 cacgttgatt gttgaagttg aagcagaaaa cggccagacc ggattcgctg tttcgacagc
     3241 cggtgaaatg ggctgtttta ttgtcgaaaa acatcttaac cgtttcattg agggtaaatg
     3301 cgtcagtgat atcaaactga tccacgatca aatgctcaat gccaccctgt attactccgg
     3361 ctctggtggc ctggtgatga atacgatttc ctgtgtcgat ctggctctgt gggatctgtt
     3421 cggcaaagtg gtcgggcttc cggtttataa acttttaggt ggtgcagttc gtgatgaaat
     3481 tcagttctac gccacaggtg cgcgtccgga tctggcaaaa gagatgggct ttatcggtgg
     3541 caaaatgccg acgcactggg ggccacatga tggtgatgcg gggatccgca aagatgccgc
     3601 tatggtcgcg gatatgcgtg aaaaatgcgg tgaggatttc tggttaatgc tcgactgctg
     3661 gatgagtcag gacgtgaact atgccatcaa actggcccac gcttgcgcgc cctataacct
     3721 gaaatggatc gaagagtgcc tgccgccaca gcagtatgaa ggttatcgcg aactgaaaca
     3781 caacgcacca gcggggatga tggtcaccag cggtgagcac cacggcacac tgcaatcttt
     3841 ccgtacgctt tcagaaaacg gtatcgacat tatgcagccg gatgttggct ggtgcggtgg
     3901 cttaaccacg ctggtggaaa ttgccgcaat cgccaaatcc agggggcaac tggtggtgcc
     3961 acacggttcg tctgtttact cccaccatgc ggtgatcacc ttcaccaata cgccattcag
     4021 cgaattcctg atgaccagcc cggattgttc aacgatgcgt ccgcaatttg atccgattct
     4081 gcttaatgag ccagtaccgg tgaatggtcg tattcataaa tcagtgcttg ataaacccgg
     4141 tttcggcgtc gaactcaatc gtgactgcaa tttgaaacgc ccctacagcc actaatcact
     4201 ccgggcgttg ctgtgacaac ggtaacgccc acatattcta aggagctttt tatgagcacc
     4261 gctttgcttg acgccgtggt gaagaaaaac cgcgcgcgtt taattccgtt tatgttggcg
     4321 ctgtatgtgc tggcgtttct cgaccgttcg aatattggtt ttgccaaaca gacctaccag
     4381 attgataccg ggctgagtaa tgaagcttat gcgctgggag caggcatttt ctttgtggta
     4441 tacgcgtttc tgggggttcc ggcgaatctt ttgatgcgca aactgggggc cagaacctgg
     4501 attggtacga caacactgct gtggggattt ctttcggctg ccatggcatg ggccgatact
     4561 gaagcgaaat ttctgattgt tcgcactctg cttggtgctg cggaggccgg gtttttccct
     4621 ggtatgattt atctcacctc gcaatggttt ccgcagcgta atcgcgccag cattatgggg
     4681 ctgttctata tgggcgcacc gctggcgtta acactgggat caccgctttc cggcgcgctg
     4741 ctggagatgc atggatttat ggggcatccc ggctggttct ggatgtttgt tattgaagga
     4801 ttgttggcag tcggcgctgg ggtattcaca ttcttttggc ttgatgacac accggagcag
     4861 gcacgttttc tgagtaaaca agaaaaaacg ttgcttatca atcaactggc aagtgaagaa
     4921 caacagaaag tgacttctcg attgagcgat gcgctgcgta atggccgagt ctggcaactg
     4981 gcgattatct acctgaccat tcaggtagcg gtttacggat taattttctt cctgccgacc
     5041 caggttgcgg cattgctggg aacaaaagtg ggctttacgg cgtcggtggt caccgccatt
     5101 ccgtgggttg cggccttgtt tgggacctgg cttattccgc gctactccga taaaaccggc
     5161 gaacggcgta atgtcgcagc gctgacatta ctggcggcgg gcattggtat tggtctgtcc
     5221 gggctgcttt ctccagtact ggcgatcgta gcactgtgtg ttgcagccat cgggtttatt
     5281 gccgtgcaac cggtgttctg gacgatgccg acacagcttc tttccggtac ggcgctggct
     5341 gcggggattg gttttataaa cctgtttggt gcagtgggcg ggtttattgc cccgatcctg
     5401 cgcgtgaaag cagaaacgtt attttccagc gatgcggcgg gattactgac gctggcagcg
     5461 gtggcggtca tcggttcgct gattattttc actctgcgtg taaatcgcac tgttgcgcag
     5521 accgacgtgg cacatcatta aatagtttaa ggaacacgat gaacgcatta ttaaccaatc
     5581 cctttaaaga acgtttacgc aagggcgaag tgcaaattgg tctgtggtta agctcaacga
     5641 ctgcctatat ggcagaaatt gccgccactt ccggttatga ctggttgctg attgacgggg
     5701 agcatgcgcc aaacaccatt caggatcttt atcaccagct acaggcggta gcgccctatg
     5761 ccagccaccc cgtgatccgt ccggtggaag gcagtaagcc gctgattaaa caagtcctgg
     5821 atattggcgc gcaaactcta ctgatcccga tggtcgatac tgccgatcag gcacgtcagg
     5881 tggtgtctgc cacgcgctat cctccctacg gtgagcgtgg tgttggggcc agtgtggcac
     5941 gggctgcacg ctggggacgc attgagaatt acatggtgca agtcaacgat tcgctttgtc
     6001 tgttggtgca ggtggaaagt aaaacggcac tggataacct ggacgaaatc ctcgatgttg
     6061 aagggattga tggcgtgttt attggacctg cggatctttc tgcgtcgttg ggctacccgg
     6121 ataacgccgg gcacccggaa gtgcagcgaa ttattgaaac cagcattcgg cggatccgtg
     6181 ctgcgggtaa agctgctggt tttctggctg tggctcctga tatggcgcag caatgcctgg
     6241 cgtggggagc gaactttgtc gctgttggcg ttgacacgat gctctacagc gatgccctgg
     6301 atcaacgact ggcgatgttt aaatcaggca aaaatgggcc acgcgtaaaa ggtagttatt
     6361 gatatcaaag gcccatgggg atcggctgtg ggcctgtgtt aattagtggt tattcgctgc
     6421 cagatcggct tcgcttagct gagtggccgc gagcacctgg tcacgatcaa tgccctgttc
     6481 cagcatgcgc aaggcaattt taatggcttg ttcatgcatg ccttctaatt taccctcctg
     6541 ccagccaatt tgatgtcctt cctgccgtaa ccgttcagca atagtcatta atctctcctt
     6601 ttaataacag ttcggaatat cagcatcagt gagttgggtt gtcgccagca caatctcacg
     6661 ttcaaagccc tgttccagca tgcgcaaggc aattttaatg gcttgttcat gcataccttc
     6721 tactttgcct ttctgcaaac cctccaattt cccctcctgc caaccttcaa ttttaccttc
     6781 ctgccagcca atttgatgcc cttcctgcca cagccgttca gcaatagtca ttaatctctc
     6841 cttttgtagt ggtgaacgtt cggcaatctc ctgaataaaa cgggtgaaac gggaggtatc
     6901 gccgcattgc agcagataat taaacagtgt ttgtagctga ctgtcattag tgaagcctct
     6961 aaccaaaagc gtggtgatcc tgtcgaccat accgattaaa tcgcggtcgc gaatatgctt
     7021 ttgaatcagt tccagcagag ctatacgccg atgttgcatg atctcatcgt caggtacgat
     7081 ggtgatatcc accaacggaa aagcttcggt gtacaactgc cgggcaagtt gcggatcatc
     7141 aaactcatcc agccagttaa gtgagtatgg gtagggcgag gtttcgccat gataaaacag
     7201 caacggcacc accagcggga ctctgtcata gcctttgtcc agatggcgct gcatggcggc
     7261 ggtggcatag cgcattagtc gaaaagccat attcttttct gcagaacttt gatgttcaat
     7321 cacgcagtag atatagccgt caccgtcgct ggtttccacc gaccacaaaa catccgagta
     7381 gtaagcgcgt aaactttttt caataaaact ggtgggttcc aggcgtaagg tttgcaggtt
     7441 gcaaagcttg cgcagtggtt ctggtaaatg tatttcgaga aaatcccgtg cggtttcggg
     7501 tgtgaacata aaggttttaa ataccgcatc atgcggggag gaggttgttg attcggtcat
     7561 ggttgtccgt cacctgatat ttatgatgac gcgacattac ggttatccca ggcctttgac
     7621 atctgccagt ttcagccttt gcgagacgtt ttcagaggaa ttttcactgc gttgcaacgt
     7681 atacattgag cgcggaatcg tgtacgcaaa aatgcccggt aaccacatgc ttaccgggca
     7741 tcagaaagga gtttaagcca gcgcctgggc cagcagcgta atcggatgtt cgcagcgaag
     7801 gcttgtcgac atctcaatct gccatttaca ggtttcgcag tcggtgacca ccagatctgc
     7861 gccgctttct tctatctggc ggaacagtgg tgcgccgatg gcttgtgagg tggggtagtt
     7921 ctcttttttg aaaccgtaag ttcccgcaat accgcagcac agggaatcca gcaccgttaa
     7981 ctcaagcccc gggattttac gcaacagctc cagggtgtag agcgtccagc ccattttttc
     8041 catatggcac ggagtgtgat aaaccacttt tagcggcagc ggtttcagcg gtaacgtttt
     8101 gccttcgtcc agcttgcgcc acagccagcg ggtcgccagt tcgatgtgat cgcgtaagcc
     8161 tttgttgtcg acattcagca cttccgggta ttcgtcgcgc agggcaaatg tacacgttga
     8221 ggaggtggca atcaccggaa tgccttttac tcccacagct tcgcggatcg actctacatt
     8281 cgtaattgcc tgtttgcgtg ctttatcggt aaaaccgttg gcgatcagcg gtacgccgca
     8341 gcatttttct ttgctgagca gttgtacgcc ggtacccatc gcgttgagca ctttaattaa
     8401 atctttgcct agctgcggat ggttgtagtt aacgaagcag ccgtgaaaga aagcgacctg
     8461 gtctttatac tgcgcttgct gagccgccac gctgcgatac cagcgacgga acgtaccgaa
     8521 ggagtatttc ggtagcgtgc gacggtggtc tatttttaac gccgcatcaa gcaactgccg
     8581 caccggtttc agcgaggtag cggtgttgac gatgggtgcg aacggcgtgg aaacgctacc
     8641 catcaggtcg gtatgactca acacaaaatt acgcagcgac gggcgtgtgg tgtcatattt
     8701 cgcccgcgcg cgctggataa tatcgccaat cttcacatcg gacgggcagg cgacttcaca
     8761 acgtttgcag ttgatgcaat atttcagcgc ctcgtcatac agtgcgccat cttttaaacg
     8821 cagacgctcg ccatccggcc cggcttgttt tggccccgga taacccggat tcacccggct
     8881 caccgggcag gcggtggtgc agacggtgca cttaatgcag ttttcgaagc tggtgtcatt
     8941 cattgttggc ctcctgcgcg ttgggcaatc tgttgagcga catgtaaagc actgacggca
     9001 caaacaccgc cgccgcatcc ctgggcgatg ggatcaaatc cgcccagcac cgaaccgatg
     9061 gcaaacaggt tctcaatggt ttgccccgcc tgcgacgggc gtagcgcctc atcagtggtt
     9121 acaccgaaca gctgccacgg ctgcggcgca aaaaaatcgc ccttatacca ttcaccccgc
     9181 gtagcggttt gtagcacatc aaggccgaga atcggctcgc gaatgccgtc acgttctgcc
     9241 accagtcctc cgctaaagaa actgccgctc gcaagcaccg caaatcgtgg gcgcagcggg
     9301 atatcggcgt gattgcgcgt ccagatttca ttcactacac catttttaca ggtcactttt
     9361 ttcacttcat cgcccggcat ccacacgcca ccctggcgca caaactggcg ctgtaactgg
     9421 ttttgcagac gaatgcccag cacggaaggc ggcagcgttg gcaaaagcat caatgaacaa
     9481 ggtagttttt cattcaacca atgccacagt ttgtcatcgg ccaggccgaa gcaggcgggc
     9541 atcaggatca tttcgcaggt attggcgacc ggaataagtg catcaagtaa caacggccag
     9601 ttttcttcat tatcaaggaa gcgggcaata ttcaccgccc ggaactcggt ggcgttattg
     9661 cgcagcacat ccagttccgg cagttcaatt tcagcggttt caactttaag atccaattcg
     9721 cgcaatgaag ctgccgcaag atgcgcctga aaatccatca ggccgctaat tcccactaca
     9781 catgttttct tcgcgggcag cggccagaca gggacttctg gcgaacttag ccaggttgag
     9841 cgcagagtgc ctagcggtgt aatccgctgg tgagccagct ctacgctgcc ctgcaattgc
     9901 gcaccgcttt cagcgattaa tgcctgcgcc tggcaagcga gatcgagcac gcgttgtggc
     9961 ccgagaagag tgtaaggatg ggcaggtgcc tgctgacgca aagattccag tccactgtga
    10021 atttctgtca ccggttgacc atccggcaga tggctcagca aatccagcga tccagatgag
    10081 aaatgcagcg cgctttgacc acgagtgaca atggcacagc gcaggccgtg tttttgcagt
    10141 tgcaggccac agagtaatcc ggcgaggccg ccgcccataa tgacagtatc aaagcgcatc
    10201 tttctgctcc ttctccagac cacacaatcc ctgataaacc cagcgggtaa attcgctttc
    10261 gcgcagtgca tctccccagg cgatgggttg cacgcctttc cagcgttcgt ttaggaaggt
    10321 ggaaagttgc tcgatagatt gcgcggacgt cgtgacgtta aaacgttgca gcagtccggc
    10381 agcgcggcag gcgcagagtt cgccctggca ggtgcccatc cccacacggg tacgacggcg
    10441 taaatccagc aggctattaa cgtttaaatt ttctaccgcg tactgcactt cacccgcagt
    10501 taccgcttcg cactcacata ccaggctacg gtgcagacgg ccttcgctca gccaggcagg
    10561 cgtgcgatcg ccatgacgat aaaccgcaga accgcgcagc ggggcaggca gggagatgac
    10621 tttacgcaag gtagcttcag cgggttcctg tgaaccagga agtgccagat cggcggtcgt
    10681 acaggggcgc gtgttgccca gtttgcggca tacggcgtcg gtagcccatt ccgccattag
    10741 ccgataggtc atcagtttgc caccggtgat ggtgataaac ccgtccagac catcgcgttc
    10801 agcatggtcg agcagcacga tgccacggct gacgttacgt ccgctcggat cgtcatcgct
    10861 ggcaaccagc gggcgtacgc cagagtaggc ccgcaaaatg cgcgttttcg ccatcacggg
    10921 ggccagtttt tccccttcac gcagcagaat atcaacttct tctgccgtca ctcgattatc
    10981 gtcaatctcg ttgtaatcaa tacgtaaaga ggtggtgcca atcagcgaaa tggtatcgcc
    11041 aggcaccaga atatcggcgt cggaaggttt gcggcagcgg ttgatcacat gctgattaat
    11101 gcggtgatcc atgatcagca gcgatccttt cgccgggaac atgcgaatgc gcagatcggc
    11161 atattcggca atgtgttgcc cccagatccc ggcggcatta actacgacag gcgcatgaag
    11221 tgcctgaatt tcgccggtga gatggttacg tacacgaaca ccgcacaccg tcgcgccttc
    11281 acgaatcagc cccgtgactt catgagcggt aaggataacg gcaccgtgtt ctttggcatc
    11341 cagcatgttt gctgctgtca gacgaaatgg atcaacggtg ccatccggaa ctttcaccgc
    11401 gccaatcagt gccgggttaa cggcaggttc gataatgcgc gcttgctgcg gatcaatgac
    11461 ttctgcgctg atccctgctt cttcgcaggc gcggataaaa gtggcctgga aggagaggtc
    11521 atcttccggc agggtgataa acaggccgtt ggttggttct acgcagtgac gtgcaatgcg
    11581 tttcaggatc tggttttcac taatacattc gcgggccgat tccgcatcgg ttaccgcata
    11641 gcgcgcaccg ctgtgcagca ggccgtggtt acgcccggtg gcaccggttg cgatgtcgtg
    11701 gcgctcaacc aaaatcacgc gcagcccgcg cagggcacag tcgcgggcaa tcccggctcc
    11761 cgttgcgcca ccgccaatga taatcacgtc acttgattgc gagtcgcgag ttttcattgt
    11821 tatccctctg aagttcgttt tttaccattt agccatagta aaaacatgaa ttgtttgatt
    11881 tcgcgcatat tcgctcataa ttcgaaagtg aaacgtgatt tcatgcgtca ttctgaacat
    11941 ttcaaccatc gtatttaata atgtgtgcgg taattcacat ttaatttatg aatgttttct
    12001 taacatcgcg gcaactcaag aaacggcagg ttctcccact gaatcaggct gttaatcata
    12061 aataagacca cgggccacgg aggctatcaa tgttgagtat ttttaaacca gcgccacaca
    12121 aagcgcgctt acctgccgcg gagatcgatc cgacttatcg tcgattgcgc tggcaaattt
    12181 tcctggggat attctttggc tatgctgctt actatttggt tcgtaagaac tttgcgctcg
    12241 ctatgcctta tctggttgag cagggattct cacgcggtga tttaggtttt gccctttcgg
    12301 ggatctcgat tgcttatgga ttttcgaaat tcatcatggg ttcggtatcg gatcgctcga
    12361 atccgcgcgt tttcctgccc gcaggtttga ttctggcggc ggcagtgatg ttgtttatgg
    12421 gctttgtgcc atgggcgacg tcgagcattg cggtgatgtt tgtactgttg ttcctctgcg
    12481 gttggttcca ggggatgggg tggccgccgt gtggtcgtac tatggtgcac tggtggtcgc
    12541 agaaagaacg tggcggtatt gtgtcagtgt ggaactgtgc gcacaacgtc ggtggtggta
    12601 ttccgccgct gctgttcctg ctggggatgg cctggttcaa tgactggcac gcggcgctct
    12661 atatgccagc tttctgtgcc attctggtgg cactgttcgc ctttgcgatg atgcgcgata
    12721 ccccgcaatc ctgtggcctg ccgccgatcg aagagtacaa aaatgattat ccggacgact
    12781 ataacgaaaa agcggaacag gagctgaccg cgaagcagat cttcatgcag tacgtgctgc
    12841 cgaacaaact gctgtggtac atcgccattg ccaacgtgtt cgtttatctg ctgcgttacg
    12901 gcatcctcga ctggtcaccg acttatctga aagaggttaa gcatttcgcg ctggataaat
    12961 cctcctgggc ctacttcctt tatgaatatg caggtattcc gggcaccctg ctgtgcggct
    13021 ggatgtcgga taaagtcttt cgtggtaacc gtggggcaac cggcgtcttc tttatgacac
    13081 tggtgaccat cgcgactatc gtttactgga tgaacccggc aggtaaccca accgtcgata
    13141 tgatttgtat gattgttatc ggcttcctga tctatggccc tgtgatgctg atcggtctac
    13201 atgcgctgga actggcaccg aaaaaagcgg caggtacggc agcgggtttt accgggctgt
    13261 ttggttacct cggcggttcg gtggcggcga gcgcgattgt tggctacacc gtggacttct
    13321 tcggctggga tggcggcttt atggtaatga ttggcggcag cattctggcg gttatcttgt
    13381 tgattgttgt gatgattggc gaaaaacgtc gccatgaaca attactgcaa aaacgcaacg
    13441 gaggctaatg gcatgaaatt gacgctgaaa aaccttagca tggcgatcat gatgagcact
    13501 atagtcatgg gaagcagtgc aatggcggcg gacagcaacg aaaaaatagt catcgcccat
    13561 cgcggtgcca gtggatattt gccggagcat acgctgccag caaaagcgat ggcgtatgcg
    13621 cagggagcgg attatctgga acaggatttg gtgatgacca aagacgacca tctggttgtt
    13681 ctgcatgacc attatctcga tcgtgttact gatgttgccg atcgtttccc ggatcgggcg
    13741 cgcaaagacg gtcgttacta cgcgatagat ttcacgctgg atgaaattaa gtcgctgaaa
    13801 tttaccgaag gtttcgatat tgaaaacggt aaaaaagtgc agacttatcc ggggcgtttc
    13861 ccaatgggta aatctgactt ccgggtgcac acctttgaag aagagattga atttgttcag
    13921 gggttaaacc actctaccgg gaaaaatatc ggtatctatc cagaaattaa agcgccgtgg
    13981 ttccatcatc aggaagggaa ggatattgcg gcaaaaacgc tggaagtgct gaagaaatat
    14041 ggttacaccg gtaaggacga caaagtttat ttgcaatgtt ttgatgctga tgagctgaag
    14101 cgtattaaga atgtgctgga acccaaaatg ggtatggatc tcaatctggt acagttgatt
    14161 gcctataccg actggaatga aacgcagcag aaacagccgg acggaagctg ggttaattac
    14221 aattacgact ggatgtttaa gccgggtgcc atgaaacagg tggcggaata tgctgatggt
    14281 attggtccgg attaccatat gttgattgag gagacatcgc agccgggtaa tatcaaactc
    14341 actggcatgg tgcaagatgc tcagcagaat aaactggtag tgcatcctta taccgtgcgg
    14401 tcagataaac tgcctgaata cactactgat gtgaatcagt tatatgatgc tctgtataac
    14461 aaagcgggtg taaatgggct atttactgat ttccctgata aggcagtaaa attccttaat
    14521 aaagagtaaa ttaactaccg gatggcactt atgtgctatc cgataccact cttcattaaa
    14581 gtctgtgggt tatccttaat tgcaattatt tagagcgaga tgacttgttc ccggcataag
    14641 atactcgtca atacaacacg tattattaag attttcctca gctaaacatc tatagtcatt
    14701 gccgttaata aaaaacgaca ggcgataggg tatggcagtt tcagcaaagc acgacgaatt
    14761 taatcactgg tgggcaacag agggcgactg ggttgaagag cctaactatc gccgtaatgg
    14821 tatgagcggg gtgcaatgcg tcgagcgcaa cggcaaaaag ctgtatgtaa agcgcatgac
    14881 gcatcatctg tttcattccg tacgttatcc gttcggccga ccaacgattg tccgtgaggt
    14941 tgcggtaatt aaagaactgg aacgggccgg tgtcatcgtg ccgaagatcg tttttggtga
    15001 agcggtgaaa attgagggtg aatggcgcgc gttgctggtg actgaagata tggcggggtt
    15061 catcagcatt gctgactggt atgcccggca tgcagtatcg ccttattctg acgaagtacg
    15121 gcaagccatg ttgaaagcag tggcgctggc gtttaagaaa atgcatagcg ttaatcgtca
    15181 gcatggctgt tgttatgttc gccatatcta tgtgaaaaca gaaggtaagg cagaagctgg
    15241 atttcttgac ctggagaaaa gtcgtcgccg cttgcgtcgt gataaagcga ttaatcatga
    15301 cttccgccag ctggaaaaat atctggagcc gatccccaaa gcggactggg agcaggtgaa
    15361 agcatattac tacgctatgt aatcgttgta ggtcagataa gacgtataag cgtcgtatct
    15421 ggcgttaaaa ccaattcaca tctcgatttc aatatcgcct tttgcccggc aacaacaggg
    15481 caaaatttcc cccggctgaa taaaagctaa cggttcggca atccagtcca cctggcctgc
    15541 gaccagtcgg gtgcgacagg agccgcagta accttcgcgg cactggtact caaccgccac
    15601 attgtgggat tccagcgccg ccagaagaga agggtgttca tcctggcaca gcagttgtgt
    15661 gccagtaatg cgcagggtaa cgcgggccat cagagctgga agttactcaa atcgtcggtg
    15721 tccacttccg cgtcaatctg cccgaccaga taagaactga cttccacttc ctgcggagca
    15781 acctgcacgt tatcagacac cagccaggtg ttgatccacg ggatcgggtt ggagcgcgtc
    15841 tggaacggca gatccaaacc gactgcctgc atacggatat tggtgatgta ttcaacgtac
    15901 tggcagagga tgtctttatt cagaccaatc atcgaaccat cacggaacag ataatccgcc
    15961 cagtctttct cttgctgtgc cgcctggaca aacaggtcat agcactcctg cttacattct
    16021 tcggcaattt ccgccatctc cggatcatcc gcgccgctgc gcagcagatt cagcatatgc
    16081 tgggtgccgg tcaggtgcag ggcttcgtcg cgggcaatca ggcgaataat tttggcgtta
    16141 ccttccatca gctcacgttc agcgaatgcg aaagagcagg caaagctgac gtagaaacgg
    16201 atcgcttcca gcgcgttaac gctcatcagg cagagataca gttttttctt cagctcgcgc
    16261 aggctaacgg ttacggtttt accgttaacg gtgtgggtgc cttcgcccag cagatgccag
    16321 tagctggtca tttcgatcag ttcatcgtaa tagctggaga tcccttccgc acgtttctgg
    16381 atctgctcgt tggtaacgat atcgtcaaag acaactgacg gatcgttaac gatattacgg
    16441 atgatatggg tataggagcg ggaatgaatc gtttctgaga acgcccaggt ttctacccag
    16501 gtttccagtt ccgggatcga gatcagcggc agcagtgcca cgttcggact gcgaccctga
    16561 atggaatcca gcagcgtctg atatttcagg ttgctgataa agatgtgttt ttcgtgttca
    16621 ggcagtgcct ggtaatcgat acggtcgcgg gagacgtcaa cttcttccgg acgccagaag
    16681 aaagagagct gcttttcgat cagcttttcg aagatgtcat atttttgctg atcgtagcga
    16741 gccacgttga ccggctgacc aaagaacatc ggttctttga gctgatcatt tttcgtctgt
    16801 gaaaaggtgg tatatgccat gaatgtgtcc tgttaggagt tttatgccgg atgcgcttcg
    16861 cttatccggc ctacaaaccg catcgtaggc tgggtagggc gttcatgccg catctgggca
    16921 tccagtgccg gatgcgcttc gcttatccgg cctacaaatt ggacccgtag gccgggtagg
    16981 gcgctcacgc cgcatccggc atcacaatat cagatcttac atgcgccgct ttcgcagcca
    17041 tcgtcctgga ttgacggcac cagatcgtcc tgcgcgtctt ctgcgccgtc acgggtgttc
    17101 tgataataca gcgttttgac cccgaatttg taggcggtga gcaggtcttt cagcaactgt
    17161 tgcatcggca cttttcctga cgggaaacgt gacggatcgt agttggtgtt agcagagatc
    17221 gactgatcga taaatttctg catgataccc accagttgca ggtagccatc gttacccggc
    17281 atttcccaca gcagctcata ggcgtcgtgc agatgctcgt agtccggcac cacctggcgc
    17341 agaataccgt ctttcgacgc tttgatgctg acgtaaccgc gcggcggttc aataccgtta
    17401 gtggcattag agatctgcga agaagtctcg gacggcatca gggcagaaag cgtggagtta
    17461 cgcaggccgt gcgttttgat tgactcacgc agtgcttccc agtcgtaatg cagcggctca
    17521 ttagcgatgg catccagatc tttcttatag gtatcgatcg gcaggatccc tttcgcgtaa
    17581 gtggtttcgt taaaccacgg gcacgcgcct tgctctttcg ccagctcatt agacgctttc
    17641 agcaggtaat actgaatggc ttcgaaggtt ttatgcgtca ggttgttggc gctgccgtcg
    17701 gagtagcgtt taccgtgctt cgccaggtag taagcgaagt tgatcacacc aatacccagc
    17761 gtacgacgac ccatcgctcc acgtttggcg gccgggatcg ggtaatcctg ataatccagc
    17821 agcgcgtcga gtgcgcgtac tgccagaatt gccagctctt ccagttcatc caggctgtta
    17881 attgcgccca ggttgaaagc agacagcgta cacagcgcga tttcaccgtt ctcgtcgttg
    17941 acgtcgttca gcggtttggt cggcagggcg atttccaggc acaggttaga ctggcgcact
    18001 ggcgcgatgg ccggatcaaa cgggctatgg gtattgcagt ggtcaacgtt ctgaatatag
    18061 atacgaccgg tagacgcacg ttcctgcatc atcagcgaga acagctcaac ggctttcaca
    18121 cgctgcttgc ggatgctgtc gtctttctca tatttggtat acagacgttc aaactcttcc
    18181 tgatcggcga agaacgcgtc gtacagcccc ggtacgtcgg acgggctgaa cagagtgata
    18241 tcttcacctt tcagcagacg ggtatacatc agtttgttga tttgaacccc gtagtccata
    18301 tgacgcacgc ggttgccttc aaccccacgg ttgtttttca acaccagcag gctttccact
    18361 tccagatgcc acatcgggta gaacagcgtt gccgcaccgc cgcgcacacc gccctgagag
    18421 caggatttca ccgcggtctg gaaatgtttg tagaacggaa tacaaccggt atggaacgct
    18481 tcgccaccgc gaatcgggct acccagcgca cgaatacgcc cggcgttgat gccgatcccg
    18541 gcacgctggg aaacgtattt aacaatcgcg ctggaggtgg cgttaatgga atccaggctg
    18601 tcgccgcact cgatcagcac gcaggagctg aactgacgag ttggggtacg cacgccggac
    18661 atgattggcg tcggcagcga aattttaaat gtggaaaccg cgtcgtaaaa acgcttcacg
    18721 tattgcaggc gcgtttcacg cgggtagttc gagaacaggc acgcggcaac cagaatataa
    18781 aggaactggg cgctttcgta gatttcgccg gtcacgcggt tctgtaccag atatttgcct
    18841 tccagctgct taacggcagc ataagagaag gtcatatcac ggtcgtgatc gataaacgtg
    18901 tccatctgct tgaactcttc ttccgtgtag tcttccagca gatgattatc gtatttgccc
    18961 atctcgacca ttttcaccac atggtcgtac agcgcaggcg gctcaaactg accgtaggct
    19021 tttttacgca ggtggaagat cgccagacgt gcggcgagat actgataatc cggcgcatca
    19081 cgggagatca ggtctgcggc agccttgata atagtttcgt ggatgtcaga ggtcttgata
    19141 ccgtcataaa actgaatgtg ggagcgcagc tcgacctggg aaatcgaaac gttatgcagt
    19201 ccttctgccg cccaatccag aacgcgatgg attttgtcga gattgatgcg ctctgtgcta
    19261 ccgtcgcgct ttgtcaccag cagattctga ttcatgtatg tcgtacctgt ttttcaatag
    19321 cttcatgcag actacctgta gtgagggaag ctgctaagaa cactatatat agggggtatg
    19381 tttgggattc actgcaagat agtgtgaaac tggccctctt gcaagtgcat aactttgtgg
    19441 ataactcagg aaggaaaaag tggcttccgc gcaccttagg tcagacaagg tgtccgggaa
    19501 agtcaatggg aagagaaaaa tttgttaaaa ataaccgttc ggtgaattct tgaacggtaa
    19561 aataggatag gtaaattgat atagatggtt tattaatctt gttgctaatg attgccaaaa
    19621 ttgatctagg cacaagtcgt cacataatct gatgtatca
//
LOCUS       DAZFFE010000091        18978 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFFE010000091 DAZFFE010000000
VERSION     DAZFFE010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.091"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..383
                     /locus_tag="ACHJ69_003495"
     CDS             <1..383
                     /locus_tag="ACHJ69_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HFO5016407.1"
                     /translation="TESPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRL
                     GGFEAIKSAYMAQVQYSMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMANFDEM
                     VPEFIEKMDEALAEIGFVYGEQWQ"
     gene            380..538
                     /locus_tag="ACHJ69_003496"
     CDS             380..538
                     /locus_tag="ACHJ69_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 family protein"
                     /protein_id="HFO5016408.1"
                     /translation="MKHPHDNIRVGAITFVYSVTKRSWVFPGLSVIRNPLKAQRLAEE
                     INNKRESV"
     gene            535..1287
                     /locus_tag="ACHJ69_003497"
                     /pseudo
     CDS             535..1287
                     /locus_tag="ACHJ69_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964734.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1295..1510
                     /locus_tag="ACHJ69_003498"
     CDS             1295..1510
                     /locus_tag="ACHJ69_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO5016409.1"
                     /translation="MTIEHNNALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            1609..1830
                     /locus_tag="ACHJ69_003499"
     CDS             1609..1830
                     /locus_tag="ACHJ69_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO5016410.1"
                     /translation="MADLIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            1827..2612
                     /locus_tag="ACHJ69_003500"
     CDS             1827..2612
                     /locus_tag="ACHJ69_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5016411.1"
                     /translation="MSEINYQELREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLIESDAQAYANAEFIADANPATVLALLDERERNQQYIKRRDQEN
                     EDIALTVGKLRVELEETKSKLNEQREYYEGVISDGSKRIAELEKIATDYALKFQKAQD
                     ALKYAVLLRKSGQEAREIKPAKGEVLVVVSGFTGCGKSAIAGEIEIAMKSIGVPVKWT
                     NGDAEKRMTGADWLTAIEMYKPTVRIVEVNVPRAAGIRIKGGA"
     gene            2612..2788
                     /locus_tag="ACHJ69_003501"
     CDS             2612..2788
                     /locus_tag="ACHJ69_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016412.1"
                     /translation="MRVAFIGLLPYPTRFWASALIAKPHVLMADNIIPAPKRRHTGIA
                     AARREAKKRRRAKR"
     gene            2964..3155
                     /locus_tag="ACHJ69_003502"
     CDS             2964..3155
                     /locus_tag="ACHJ69_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016413.1"
                     /translation="MKNRKAKMLISRVYRRCYPSQWLRVSNRRVVLYLYSGIAREGIK
                     DKRSAAQNRWKKHLRTKGA"
     gene            3157..3366
                     /locus_tag="ACHJ69_003503"
     CDS             3157..3366
                     /locus_tag="ACHJ69_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016414.1"
                     /translation="MSIAASYTMHLYCDCRQCTEGVYPVPDFGEYIGTSWSGCAKEDR
                     KDGWRISKDKTRAFAPGHKVLRINT"
     gene            3363..3488
                     /locus_tag="ACHJ69_003504"
                     /pseudo
     CDS             3363..3488
                     /locus_tag="ACHJ69_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF550 domain-containing protein"
     gene            3486..3758
                     /locus_tag="ACHJ69_003505"
                     /pseudo
     CDS             3486..3758
                     /locus_tag="ACHJ69_003505"
                     /inference="COORDINATES: protein motif:HMM:NF016342.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dATP/dGTP pyrophosphohydrolase domain-containing
                     protein"
     gene            3942..4172
                     /locus_tag="ACHJ69_003506"
                     /pseudo
     CDS             3942..4172
                     /locus_tag="ACHJ69_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            4257..4499
                     /locus_tag="ACHJ69_003507"
     CDS             4257..4499
                     /locus_tag="ACHJ69_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5016415.1"
                     /translation="MFALIQRGQIYTDSAGYPIKIVRCINNTVLYRRMDGRTQSVKIN
                     DFNELFERIDHQEYRQILAETEQEAHLKKLRAMKRK"
     gene            4503..4637
                     /locus_tag="ACHJ69_003508"
     CDS             4503..4637
                     /locus_tag="ACHJ69_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016416.1"
                     /translation="MNKAFERWVHQRYSNRYDLTRDVDGFYCREVVKRMFDVWCHCRG
                     "
     gene            4656..4910
                     /gene="xisR"
                     /locus_tag="ACHJ69_003509"
     CDS             4656..4910
                     /gene="xisR"
                     /locus_tag="ACHJ69_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO5016417.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            4944..6230
                     /locus_tag="ACHJ69_003510"
     CDS             4944..6230
                     /locus_tag="ACHJ69_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5016418.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKAG"
     gene            6251..6952
                     /gene="mlrA"
                     /locus_tag="ACHJ69_003511"
     CDS             6251..6952
                     /gene="mlrA"
                     /locus_tag="ACHJ69_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO5016419.1"
                     /translation="MSLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYISICLASARKKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGDNRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            7012..7119
                     /gene="yohO"
                     /locus_tag="ACHJ69_003512"
     CDS             7012..7119
                     /gene="yohO"
                     /locus_tag="ACHJ69_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO5016420.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(7100..7831)
                     /gene="yehW"
                     /locus_tag="ACHJ69_003513"
     CDS             complement(7100..7831)
                     /gene="yehW"
                     /locus_tag="ACHJ69_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO5016421.1"
                     /translation="MKMLRDQLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLKPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGANTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQAFSQHAK"
     gene            complement(7836..8762)
                     /gene="yehX"
                     /locus_tag="ACHJ69_003514"
     CDS             complement(7836..8762)
                     /gene="yehX"
                     /locus_tag="ACHJ69_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO5016422.1"
                     /translation="MIEFSHVSKLFGTQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLQGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLAMLTHPANDFVRQFFGHSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGEPCGTLHFQDLLEEA"
     gene            complement(8755..9912)
                     /gene="yehY"
                     /locus_tag="ACHJ69_003515"
     CDS             complement(8755..9912)
                     /gene="yehY"
                     /locus_tag="ACHJ69_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO5016423.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTLWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIVPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(9919..10836)
                     /gene="osmF"
                     /locus_tag="ACHJ69_003516"
     CDS             complement(9919..10836)
                     /gene="osmF"
                     /locus_tag="ACHJ69_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO5016424.1"
                     /translation="MPLSKLWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDTVWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLAELSRYLKEGGNF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLRPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(11026..13323)
                     /gene="bglX"
                     /locus_tag="ACHJ69_003517"
     CDS             complement(11026..13323)
                     /gene="bglX"
                     /locus_tag="ACHJ69_003517"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO5016425.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKAEFELL"
     gene            13519..15234
                     /gene="dld"
                     /locus_tag="ACHJ69_003518"
     CDS             13519..15234
                     /gene="dld"
                     /locus_tag="ACHJ69_003518"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO5016426.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHGHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(15273..16205)
                     /gene="pbpG"
                     /locus_tag="ACHJ69_003519"
     CDS             complement(15273..16205)
                     /gene="pbpG"
                     /locus_tag="ACHJ69_003519"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO5016427.1"
                     /translation="MPKFRVSLFSLALMLTVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(16379..16966)
                     /gene="yohC"
                     /locus_tag="ACHJ69_003520"
     CDS             complement(16379..16966)
                     /gene="yohC"
                     /locus_tag="ACHJ69_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO5016428.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            17136..17714
                     /gene="yohD"
                     /locus_tag="ACHJ69_003521"
     CDS             17136..17714
                     /gene="yohD"
                     /locus_tag="ACHJ69_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO5016429.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWSLIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(17834..18595)
                     /gene="yohF"
                     /locus_tag="ACHJ69_003522"
     CDS             complement(17834..18595)
                     /gene="yohF"
                     /locus_tag="ACHJ69_003522"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5016430.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDGGDVKPDAEPSIPLRRFGTTHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLVNPQFNPE"
     gene            complement(18648..>18978)
                     /locus_tag="ACHJ69_003523"
     CDS             complement(18648..>18978)
                     /locus_tag="ACHJ69_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="may be involved in resistance to puromycin,
                     acriflavine and tetraphenylarsonium chloride; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO5016431.1"
                     /translation="LDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQA
                     QIERDALRVVGLAQARFNAGLIAGSRVSEARIPALRERAHGLLLQGQWLDASIQLTGA
                     LGGGYKR"
BASE COUNT         4884 a         4925 c         4806 g         4363 t
ORIGIN      
        1 ttactgaatc cccgatcatc tatcgcgacg aaagtatgcg cacagcctgc tctcccgatg
       61 gtttatgcag tgacggcaat ggccttgagc tgaaatgccc gtttacctcc agggatttca
      121 tgaagttccg gctcggtggt ttcgaggcca taaagtcggc ttacatggcc caggtgcagt
      181 acagcatgtg ggtgacgcga aaagatgcct ggtactttgc caactatgac ccgcgtatga
      241 agcgtgaagg cctgcattat gtcgtgattg agcggaatga aaagtacatg gcgaattttg
      301 acgagatggt gccggagttc atcgaaaaaa tggacgaggc actggctgaa attggttttg
      361 tatatgggga gcaatggcaa tgaagcatcc tcacgataat atccgggtag gcgcgatcac
      421 tttcgtctac tccgttacaa agcgaagctg ggtatttccc ggcctttctg ttatccgaaa
      481 tccgctgaaa gcacagcggc tggctgagga gataaataat aaacgggaga gtgtatgatt
      541 cattttcacg gtggtccaat aactcccgat acctgtgcgt tgaaagcctg gaaaggcaga
      601 cacgcattca tcagttttgc taatccagct cagattgatc tggcttccga agtcacccaa
      661 tcatttgctc ttgataatgg tgcattcaca ttctggacaa aaaacaaggc catagactgg
      721 aatgaatatt acagatttgt tgaacgctgg ggtaatcacc ctcgtttctc attcgcggtt
      781 atcccggatg ttatcggcgg aaccagtgaa gagaatgacg ccctgattgc ggcatggcct
      841 cacggtaaat ttattggtgc tccggtgtgg cacatgaacg aaccagatga gcgatttatt
      901 cgtctgtgcc atgagtttcc ccgcgtctgc atcggctcga tgggggaata cgatgcaaaa
      961 cgaccgagag cgtgtcgggc taaactacgc gatcttatac gtcatgttgt agatcagtac
     1021 ggctacccaa tcaccaagat tcatgggtta cggatgttaa ataaagacat ttttactcat
     1081 gtaccgcttt cgtctgcaga cagcacgaac gtcgcaagaa atataggcat cgacaaatcc
     1141 taggtaggtt cgccatatgc tcccgcaagt aaagaaaccc gtacgcaagt tcttgtggaa
     1201 cgcattgaat cattcaacag tgccagttca cttaattaca acgctgaacg ggacgtcttt
     1261 acccctcaac tggcattcga agtgtgaggc caatatgaca atagaacata ataacgccct
     1321 tcgcagcatt gcccgtcagg ctaattctga aatcaaaaaa gccagacagc agtttccgga
     1381 taaaaacgtc gatgacattt gccgtagcgt actgaagaag caccgcgaaa cggtaacgct
     1441 gatgggattc acaccgactc acttaagtct ggcaatcggt atgttaaacg gcgtctttaa
     1501 ggaacggtga acatgaaaag caaaatcatc agggagctac aggctccttt tttattattc
     1561 gcattcaccc tcaagcgtat taaccaacaa ttcagggatt aatggaagat ggcagacctc
     1621 attgattcag catcagaaat tgaagaatta cagcgcaaca cagcaataaa aatgcgccgc
     1681 ctgaaccacc aggctatatc tgccactcat tgttgtgagt gtggcgatcc catagatgag
     1741 cgaagacgcc tggccgttca gggttgtcgg acttgtgcaa gttgccagga ggatctggag
     1801 cttatcagta aacagagagg ttcgaagtga gcgaaattaa ctatcaggaa ctgcgtgaaa
     1861 aggcagagaa agcaactaaa ggaagctaca tcgtagggca tacatctgtt aaccagcacg
     1921 gcaatttaac aggagttttt gtttgtcaaa aatggaaagg agaacccggt ggcgtgattg
     1981 ccgaatgtca tgttaactgc ctgattgaat cagatgctca ggcttatgca aacgctgaat
     2041 ttatagcaga tgctaaccca gctaccgtac tggcgctact ggatgaacgg gaaagaaacc
     2101 agcaatacat caaacgccgc gaccaggaga acgaggatat tgcgcttacg gttgggaagc
     2161 tgagagttga gcttgaggag acaaaatcaa aactcaacga gcagcgtgag tattacgaag
     2221 gtgttatctc ggatgggagt aagcgtattg ctgaactgga gaaaatcgcc actgactatg
     2281 cactgaaatt tcagaaagcc caggacgcat taaagtacgc tgttttgctg cgcaaatcag
     2341 ggcaggaagc acgggaaata aaaccagcca aaggcgaagt tcttgtcgtt gtatctggtt
     2401 ttactggttg cggaaaaagc gccatcgccg gggaaataga aatcgcgatg aagtctattg
     2461 gtgtaccggt taagtggact aatggcgatg cggaaaagcg catgacagga gctgactggc
     2521 tgacagcgat tgagatgtac aaaccaacag tgcgcatcgt ggaagttaat gtgccacgcg
     2581 ccgcaggcat tcgcatcaaa ggaggtgcgt aatgcgtgtc gcgtttatcg gtttattacc
     2641 atacccgact cgtttttggg cttctgcgct aattgcaaag ccgcatgtac tgatggctga
     2701 caacatcatc ccggcaccaa agcgccgcca taccggtatt gcggctgcac gacgcgaagc
     2761 aaagaaacgc aggagagcaa aacgatgaaa aaccgtaagg caaaaattct gttagctcgc
     2821 agaaacggtg ttggggtctg gcgctggttg aggattagta acagacgagt gaggttgacg
     2881 gggtgttgcg gggtgatggg gcacagctgt tgcaaaaagc ccagcgcgtc acagaacagg
     2941 cggaagaaag ggtacgcagt aagatgaaaa accgtaaagc aaagatgctt atttcccgtg
     3001 tatacagacg ttgctatccc agccagtggt tgagagttag caatcgccgt gtggtgttgt
     3061 acttatattc tggaattgcc agagagggaa tcaaagataa gcgcagcgcg gcgcaaaacc
     3121 gctggaaaaa acacttgcgc actaaaggag cctgatatgt ctattgccgc aagttacacc
     3181 atgcatctct attgtgactg ccgccagtgt acggaaggtg tatatccagt gccagacttc
     3241 ggtgagtata tcggtacgtc atggtctggt tgtgcaaaag aggaccgtaa agacgggtgg
     3301 cgaataagca aagacaaaac acgtgctttt gcgccagggc ataaagtttt aaggattaac
     3361 acatgaccac tattaccaaa gaacgtattg aattgttcat taaaaatccg cttgaaaacg
     3421 ggcttactcg tggcgaacaa atggaactgg cacgaattgc actggcatca ctggaacgcg
     3481 aactgattcg ccacgagcat gccaaatggt ctgactccac atttggctgc gttggcccca
     3541 ttggtccact gaaacacctc tcaaaagagg ctctggaagc cgcagccgaa ccagacgatc
     3601 tcagcgagtg ggctgatatg catttcctgt tgtgggatgc acagcgccgt tctggcatca
     3661 gcgatgctga aattaccgct gctatggaag ataaattgaa gatcaacatg gagcgccagt
     3721 ggcctgaacc aaaagatggt gagcctcgct tgcacattaa agaacccggc aactctccgg
     3781 taattccgga tggtttatcc acggtacgag ctgaggctta tcaggttgta ggagttatgg
     3841 cagatgcgct tggtgtattc ggtgatgcag cagtacagaa agttctggat aacctgtcac
     3901 agcaaaaact tgttcacaga gatgtgctgc cgttctcgct tccggtaact ccggatggtt
     3961 ggataagctg tagtgagcga atgccgaacg ataaacagta tgtttggtgt tggggtaagt
     4021 cttacggctg gactgagtgc gataccttcg aagggtatta cgattgttcg agaaacaaat
     4081 ggtgggcagt tactgacaat ggggaagaac cggcatcgaa agtaacccac tggatgccgc
     4141 tgccagaacc gccgcaggag gtgaatcaat gagctggcct gatgcaatcg taactctggg
     4201 gatggtattc gcagtagcgt ttgttgtgta ctcgatttgt cgatggggat aaccacatgt
     4261 tcgctttgat tcaacgcggg cagatataca ccgatagtgc tggctacccg ataaaaattg
     4321 ttcgctgcat aaacaacact gtgttgtaca gaagaatgga tgggcgaaca cagtcagtaa
     4381 aaataaacga tttcaatgaa ctgtttgaac ggattgatca ccaagaatac cgacaaattc
     4441 tggcagaaac agagcaggaa gctcatctga aaaaattacg ggccatgaaa aggaagtaaa
     4501 caatgaataa agcatttgaa cgatgggtcc accagcgtta cagcaatcgc tatgatctga
     4561 cgcgagatgt tgacggtttc tactgtcgtg aagttgtgaa gcgaatgttt gacgtgtggt
     4621 gccactgccg tggatgaaaa ttttatgagg ttggcatgca gacaatcatc tatcagataa
     4681 cccccagcaa atggtgtacg gagagagtcc tcattgcatc aacagggcta aagcctggca
     4741 ccattgagcg ggcaagaaga aagtcatgga tgcagggaaa agaataccgc cattacgctg
     4801 tagaaggtga tccggggcac tacagtgaat gcctgtacaa catcgaagaa attatgcgat
     4861 ggatcgaaaa ccagaaacaa ccaggtgcca aaaatgcaag ttccggttaa cctgttaatg
     4921 ctcctggacg tctgggaggt ttaatgagta acgcatcata cccgacaggc gttgaaaacc
     4981 atggaggatc actccgtata tggtttcact ataatggcaa acgtgtcaga gaaaacctcg
     5041 gtgttcctga cacagccaaa aaccggaaga tcgctggtga acttcgcact tccgtttgtt
     5101 ttgcaatcag aatggggagt ttcgactacg ccgcgcagtt ccctaattcc cctaacctga
     5161 aacactttgg tctgggaaaa agagagataa ccgttaaggc actttcggaa aaatggttgg
     5221 accttaagaa aattgagatt tgtgcgaatg cacttaaccg ttaccagtca gtaattaaaa
     5281 acatgttacc aatgttaggt gaaaaaaaac tggtttcatc cataacaaaa gaggatttac
     5341 ttttcgtaag gagagatttg ttgaccggtt accaaaagct ttctaatgga aagacttctt
     5401 ccataaaagg gcgctcagtg gtcacggtaa actactatat gacaaccata gctggaatgt
     5461 ttcaatttgc aacagataat ggttatacct caggaaaccc atttaacggt ctggccccct
     5521 taaaaaagtc caaggtaaaa ccagatcctc tcacccgtga cgaatttatt cgttttattg
     5581 aagcttgccg tcatcaacaa acaaaaaacc tgtggattct cgctgtatac acgggtattc
     5641 gtcacgggga gctggtatcg ctggcatggg aagatataga tcttaaagca aggactataa
     5701 ccatccgtag gaattataca aaacttggcg aattcactcc accaaaaacc gatgctggca
     5761 ccggaaggac aattcatctg gttcaaccag ctattgatgc tcttaaaagt caggcggaaa
     5821 tgaccatgct tggaaagcaa cattctgtag aggtaaagca gagggaatat gggagaagta
     5881 ctgtgcataa atgcactttt gtttttagtc ctcaggtaat aaaacagcgg cagttttccg
     5941 gaccgcacta taaggttgac tccatcaggg agtcatggac aagtatctta aaacgcgcag
     6001 gtctgagaca cagaaaatcg taccaatcca ggcatactta tgcatgctgg tcacttgccg
     6061 ctggagctaa tcctagtttt atcgcaagcc agatgggcca cacaaacgca caaatggtat
     6121 tcaatgttta cggagcatgg atgaaagaca acaatcacga acagatagaa ctccttaaca
     6181 aaagactatc tgaaagtgtc ccatgtatgc cccataagaa agctgggtaa aataaaaact
     6241 tgcaaaatca attagtttac ccttaatccc tgtcacgtta cgcgcgtggc agaggcgtta
     6301 cggattgctg aaaccgcaac ggacagacgg cggtcatcgg ctattcaacg atgccgatat
     6361 tgaccggatc cgcgagatca aacgctggat cgacaacggc gtgcaggtca gcaaagtaaa
     6421 aatgctgctc agtaatgaaa atgttgatgt gcagaacggc tggcgcgatc agcaagaaac
     6481 attactgact tacctgcaaa gcggcaatct gcatagcctg cgaacgtgga tcaaagagcg
     6541 cggtcaggat taccccgccc agacactcac cacacatctg tttattcctc tgcgccgacg
     6601 gcttcagtgc caacaaccga ctctccaggc gctgctggcg atcctcgacg gcgtactgat
     6661 caactacatc tctatttgcc tggcttcggc gcgcaaaaaa cagggtaaag atgcactggt
     6721 ggttggctgg aatattcagg ataccactcg tctgtggctg gagggctgga ttgccagtca
     6781 acaaggatgg cgcattgatg tcctcgccca ctcgctcaat caactacgcc ctgaactgtt
     6841 cgaaggccgt acattgctgg tatggtgcgg tgataatcga acctccgccc aacagcagca
     6901 actcaccagt tggcaggaac aaggccatga tattttccca ctcggcattt aatgattcgt
     6961 taacaaatac gctttactgt acaatccttt cgttaacata aggagtgcat tatgcgcata
     7021 gctaaaattg gggtcatcgc cctgttcctg tttatggcgt taggcggaat tggtggcgtc
     7081 atgctcgcag gttatacctt tattttgcgt gctggctgaa cgcctgcacc agcctttcaa
     7141 acaggcggtc tgcgatgatc gccgccagtg ccaccagtaa cgctccctgg atcacatacg
     7201 cggtattaaa tccgctaagc ccgatgatga tcggcgtacc cagcgtgttg gcccctaccg
     7261 ttgaggcgat cgtcgccgtg ccaatgttga taatcaccga agttcgtaca cccgccagaa
     7321 tcaccggagc tgccagcggt agctcgacct tacgcagtcg ctgaccacga ctcattccca
     7381 tacctttcgc aacttctgtc acgctggcat caatcgctcc cagcccggca agtgtcgcct
     7441 gcaggacggg cagcacgccg taaaggatca aggcgataat cgctggtttc agaccaaagc
     7501 cgatcaccgg aacggcaatt gccagcaccg cgacgggcgg aaaagtctgc ccaacggcgg
     7561 caatagtttc caccagtggg cgaaattccg caccccacgg gcgagtgaca gcaattccgg
     7621 caccagtacc aatgatcacc gcaaacaaac tcgaaattcc caccagccag aaatgagcca
     7681 gtgccagagc tgcaaagctt tcctgctgat aaacgggtcg tggcagttgt gggaacaagg
     7741 cagcaaacag cggctggctg taaggcagcc agaaaatcag cgccacaaac agagcaatca
     7801 gccagaacag ctgatcgcgc aacatcttca tacgattacg cctcctccag cagatcctga
     7861 aaatgcagcg tgccgcaagg ctcgccctgc gtgttcacca ccggcagcac ctcgcatccc
     7921 cgcgcgacaa acagagaaag cgcatcgcgt agcgtcatct cttctgccag cgcctcacct
     7981 tctgcccgtt cttcgcgacg cacgtaatcc gccacgctac gtaacgaaag caggcggaca
     8041 cccagttcac tatgcccaaa aaactggcgg acaaaatcat tcgccggatg agtcagcatc
     8101 gccagaggat tgccctgctg tacgacttca ccgtgatcca tcaataccag atgttctgcc
     8161 agccgtagcg cctcatcaat atcatgagtg accagcacaa tagtgcgccc ctgcaaacgg
     8221 tgaatgcgcg tcatctcttg ttgcaacgcg ccacgcgtta ccgggtccag cgcgccaaaa
     8281 ggttcatcca tcagtaagac ttgcggatcg gcagccagtg cgcgcgccac tcccacacgt
     8341 tgctgctgac caccggaaag ctgatgcgga taacgctcac gcaaatttgg ctccagcccc
     8401 agtagcgcca ttaattcgtc gatacgatcg tcgatccgcg cccgtgacca tttttgtaat
     8461 tgcggcacgg tggcaatatt ttgcgccacg ctccagtggg gaaacaggcc aatagattga
     8521 atggcatagc ccatccggcg gcgcaactcc agcactggca gcgagcgaat ttcttctccg
     8581 gcaaagcgga tcacgccact gtcatgctcc accaggcggt taatcatttt cagagtggtg
     8641 gatttaccgg agcctgatgt accaatcagc accgaaaaac tcccctcctg aaaattgaga
     8701 ttgagatcgt taacggcttt ttgtgtgccg aacagtttgc tgacatggct aaattcaatc
     8761 attacgtttc accttcagca gtgcgataag caaatcgaac agcgcgtcgg taagcaccgc
     8821 cagaacaatt accgggatca cccccagcaa cactaaatca atggcactgc ttaacagccc
     8881 ctggaaaacc agcgcaccaa aaccgcctgc gccgattaac gctgcaatca ccgccatgcc
     8941 aacggtttgc accatcacca cccgcaggct gcgcagaaat accggtaacg ccagcggcaa
     9001 ctgaacatgc aggaaacgct gcgccccgct catccccatc gctctggcgc tctccagcac
     9061 atcgcgcggg atctggttca agccgactac cacgccgcgc accagcggca gcaaggcata
     9121 gagcaccagc gcaatcagtg cgggtgtcat tccggttcct gctatgccga gcgttcccag
     9181 ccacggaaag gccgtaacca gcgcggcaag cggcgcaatc aacaggccaa agagcgctac
     9241 cgaaggcacg gtctgaatga cattcagcag agaaaaaatc gccccttgcc gcgcggtgga
     9301 aaagtagcac cagatgccca acggcacacc aatcactaac gcaggcagca ccgcaccaaa
     9361 cagcaacgtc agatgttgtg ccagcgcgtc gtcaaacaca tcctgacggt tggcgtattc
     9421 tttcattaac gagagatcgt taagcgtgcc ggagtacagc aaccacagcg gaacaatggc
     9481 aatctgcata tgcaacaacc agcgccacag cggatgcgtg gagattcggc ggatggcatc
     9541 gctacaggcc agcagtgcca gcgccgcagc cagccagaaa ccactgccga ggctggtacg
     9601 cgccagcgca ctaccatttt gcgccagttg tgtcgccgcc tttccagctc cccacaccag
     9661 caatacgaag acgaattgcg ccagaatgag tgcaaaaatg ctgccttttt tagcgggaat
     9721 aaaacaggct gtcagccagg cgcaaccaac gcccaccagc atccagagag tttgcggcca
     9781 cagctgccag agatgacgcc cctcacccga aactaaacga ttaggtgcgt aactgataaa
     9841 cggcagcgcc gctgcgattg ccgtcagcaa cagcagcagc gccagaacag gattaatacg
     9901 gaaataagtc acgggaaatt acttcgtcca ccctttttgt ttcaggtagt cggctgccac
     9961 ttttttggca tccagtcctt ccactgcaat gctggcattc agctgctgca atgttttttc
    10021 atcgaggctg gcgaagactg gccgtagcca ctgtgccatt tgcggatact ccttcaacac
    10081 cgattcacgc accactggtg caggcgcgta gataggttgc acgccttgcg gatcgcttaa
    10141 ggtttgcagc cccagcgccg caaccgggcc gtcggtgccg taagccattg cggcattaac
    10201 gccagaagtt tgctgggcag cggctttgat cgtcaccgcc gtgtcaccac cggccagtga
    10261 cagcaactga tcctgaccga gcttaaagcc ataggctttt tcaaacgcgg gtaaggcatc
    10321 ggcgcgttcg ataaactctg ccgaggctgc cagtttgaag ttgccaccct ctttcagata
    10381 acgactcagc tcggcaagcg aagtgagttt gtttttctct gccacatcct gacgcacggc
    10441 gatggtccag gtgttatttg caggcgcggg cgtcagccag attaacttgt tttgctctgc
    10501 atcgagtttt ttgactttct catagccttg ctgcgcgttt ttccataccg tatcgttttc
    10561 atctttaaag aaaaacgcgc cattgccagt atattccgga tagatatcca gctcgccaga
    10621 agtgatcgcc ccgcgcacta cgggagtcgt tcccagctgc actttattta ccgtaggaac
    10681 gccgtgactt tccagcacct gcaaaatgat attgccgagc agcgcgcctt ccgtatcgat
    10741 ttttgaacca accttaacgg gggaagccgc ctgtagcggc aggctcacgg ctgccaacaa
    10801 aaccagtgaa cctgcccaga gctttgagag tggcatgatg ctttcctcat tcttttactg
    10861 ttgttttcag cgaattaaga gaaaagcata gttgataatg acagggttag cctgtctgga
    10921 cggattcagt tgcagaatca gataaatact taaagggcat attttgcccg gcgtttcttg
    10981 ccggatgcgg cgtaaacgcc ttatccggcc tacaaacatc gaaaattaca acaactcaaa
    11041 ctcggctttc ttaacgcgtg cggaatcagt gccgatgaag acattgaact tgccaggctc
    11101 ggcgtcatat ttcatctgtt gattccagaa cttcagcgcc tcaatatcga ttgggaagct
    11161 gacggtctga gtttcgcccg gtttcagggt gattttctca aagcctttaa gctgcttcac
    11221 cgggcgactc atggaagccg tcacatcctg caagtacatc tgtactaccg tggccccttc
    11281 gcgtttaccg gtgttcgtca cctgcacgct ggccgtcact ttgccgtcac gcttcatggt
    11341 cggcgcagaa agtttcacat cagagacggt gaaagtggtg tagctcagac catagccgaa
    11401 cggataaagc gccccgttag cttcatcaaa ataacgcgaa gtgtatttgt tcggcttgtc
    11461 ggcattatac ggacgcccgg tattcagatg gctgtagtac accgggatct gcccgacaga
    11521 acgcgggaag gacatcggca gcttgccgga cgggttataa tcgccaaaca atacatcggc
    11581 aattgcatta ccgccttcag tccccgcaaa ccaggtttcc agaatcgcat cagcctgctg
    11641 atcttctttc accagcgcca gcggacgccc gttcatcagc accagcacca gcggtttacc
    11701 ggtggctttc agcgccgcaa tcaagtcacg ttggctttgc ggaatagtga tatcggtacg
    11761 actggaagct tcgtgcgcca tcccctgagc ttcaccgacc acagccacca ccacatcaga
    11821 ttgtttcgcg gtttgcactg cttcatcaat catctcttgc ggcgaacgcg gatcaacttt
    11881 gaccgcctct tcatattgat tcaggaaatc gataatgcct ttgtcactgg taacgttcgc
    11941 ccctttggca tacagcactt taccgttttc accgacggcg tttttaatcc cggttagcac
    12001 agtaacagat tgatcggcga cacctgccgc cgaccagctt cccatcacgt cacgcttgct
    12061 gtctgccagc gggccaacca ccgcaatggt tgcagatttt ttcagcggca gcgtttcgag
    12121 acgatttttc agcaacacca ggctttcgcg cgccacttca cgcgcttctt tgcggtgcag
    12181 gcggctttcg gcattggtat ccaccgggtc agactctttc ggccccaaat ggctgtatgg
    12241 gtcgttaaac aatcccatat catatttaac gttcagtaca tggcgggcag catcgtccag
    12301 ctcttccatc gtcactttgc cggatttgat caacccaggc agatacttcg agtaatactc
    12361 gtcgctcatg ctcatgttga ttccggactt cagcgccacg cgtaccgcat cttccgggtc
    12421 tgccgccgtg ccatgtttaa tcagctcttt gattgcgccg tgatcggaaa cggtgatgcc
    12481 tttaaagccc cattggtcgc gcagaacatc tttcagcagc caggagtcgg aggtggctgg
    12541 cgtgccgttc agcgagttca gcgccaccat caccgcaccg ctgcctgcat ccagccccgc
    12601 tttgtacggc ggcatatagt cgttaaacag gcgctgcgga ctcatatcga cggtgttgta
    12661 ctctttaccg ccttctaccg cgccgtatgc ggcaaagtgt ttgacgctgg tcatcaccga
    12721 gtagcgatct gccgggcttt taccctgcat cgcttccacc atggttttgc ccattattga
    12781 ggtgagatac gtatcttcgc caaaaccttc ggaagcacgt ccccagcgcg gatcgcgcga
    12841 gacatcgacc atcggtgccc aggtcatatt caggccatca tctgccgctt cataagcaga
    12901 gacacgcccg actgttttca ccgcatcgag gttaaaagac gaagccagac cgagactaat
    12961 cgggaacacc gtgcgctgac cgtgcagcac atcgtaagca aagaaaagag gaattttcag
    13021 gcggcttaat tccatcacct gatcctgcat ggcgcggata tcctgacggg ttacggtgtt
    13081 gaaaatcgcc ccaacctgac cgtctttgat catctcgcgg atcgcctctt tcgggttatc
    13141 cgggccgacg ctgattaagc gcagctgacc aattttctca tcaactgtca ttttcttaag
    13201 cagttcggtg acgaacgcat cccgcgcttc gggcgttaat ggatggttgc cgaataaatc
    13261 atccgccagt gctggctgca gggccagact caccgcgatt cctactgaac atagccattt
    13321 catatggatt tttcttcctt tgttgcccga cgtggcagcg aaaatggtgc aaaaaccgta
    13381 gtttgccata agcatgatgg agagagaaaa agaatgctca gtttattgtc tgaattttca
    13441 aaatattcac tcgctgaatt gtcatacaag gcgctattct agtttgtgat attttttcgc
    13501 caccacaagg agtggaaaat gtcttccatg acaacaactg ataataaagc ctttttgaat
    13561 gaacttgccc gtctggtcgg tcattcacac ctgctcaccg atcccgcaaa aacggcccgc
    13621 tatcgcaagg gcttccgttc tggtcagggc gacgcgctgg ctgtcgtttt ccctggctca
    13681 ctactggaat tgtggcgggt gctgaaagcc tgcgtcaccg ctgataaaat tattctgatg
    13741 caggctgcca atacaggcct gaccgaagga tcgacgccaa acggtaacga ttatgatcgc
    13801 gatatcgtga tcatcagcac cctgcgtctc gacaagctgc acgtactcgg taagggcgaa
    13861 caggtgctgg cctatccggg caccacgctc tattcactgg aaaaagccct caaaccgctg
    13921 ggacgcgaac cgcactcagt gattggatca tcgtgtatag gcgcatcggt catcggcggt
    13981 atttgtaaca actcgggcgg ctcgctggtg caacgtggcc cggcgtatac cgaaatgtca
    14041 ttattcgcgc gtataaatga agatggcaaa ctgacgctgg tgaaccatct ggggattgat
    14101 ctgggcgaaa cgccggagca gatccttagc aagctggatg acgatcgcat caaagatgac
    14161 gatgtgcgcc acgatggtcg tcacggccac gattatgact atgtccaccg cgttcgtgat
    14221 attgaagccg acacgcccgc acgttataac gctgatccgg atcggttatt tgaatcttct
    14281 ggttgcgcag ggaagctggc cgtctttgcg gtacgtcttg ataccttcga agcggaaaaa
    14341 aatcagcagg tgttttatat cggcaccaac cagccggaag tgctgaccga aatccgccgt
    14401 catattctgg ctaatttcga aaatctgccg gttgccgggg aatatatgca ccgggatatc
    14461 tacgatattg cggaaaaata cggcaaagac accttcctga tgattgataa gttaggcacc
    14521 gacaagatgc cgttcttctt taatctcaag ggacgcaccg atgcgatgct ggagaaagtg
    14581 aaattcttcc gtccgcattt taccgaccgt gcaatgcaaa aattcggtca cctgttcccc
    14641 agccatttac cgccgcgcat gaaaaactgg cgcgataaat acgagcatca tctgctgtta
    14701 aaaatggcgg gcgatggcgt cggtgaagcc aaatcgtggc tggtggatta tttcaaacag
    14761 gccgagggcg atttctttgt ctgtacgccg gaggaaggca gcaaagcgtt tttacaccgt
    14821 ttcgccgctg cgggcgcagc aattcgttat caggctgtgc attccgatga agtcgaagac
    14881 attctggcgc tggatatcgc tctgcggcgt aacgacaccg aatggtatga gcatttaccg
    14941 ccggagatcg acagccagct ggtgcacaag ctctattacg gccattttat gtgctatgtc
    15001 ttccatcagg attacatcgt gaaaaaaggc gtggatgtgc atgcgctgaa agaacagatg
    15061 ctggaactgc ttcagcagcg tggcgcgcaa taccctgccg aacataacgt cggtcatttg
    15121 tataaagcac cggagacgtt gcagaagttt tatcgcgaga acgatccgac caacagtatg
    15181 aatccgggga tcggtaaaac cagtaagcgg aaaaactggc aggaagtgga gtaataataa
    15241 cggatggcag agtatcgcca tccgtcattc atttaatcgt tctgtgccgt ctgccccgcc
    15301 gctgccattt gggcggcttt ttgtttttta tagctcaacg ctgctgccgg aacaggcatc
    15361 actttaccgg tttcaatcca ggtacgcagg cggctggcat cggcaaaatg ggtatatttg
    15421 ccaaacgcgt ccatcactac cagcgccacc ggtttattat tgataaccgt gcgcatcacc
    15481 agacaatggc ccgccgcatt ggtaaaaccg gttttggtta actgaatatt ccagttatca
    15541 cgatacacca gatggttagt attgcggaac ggcagcgtat acgtcgggtt agagaaggtt
    15601 gccatatctt cccgggtagt acttaactgc ccgatcaacg gatattgttt gctggcaata
    15661 agtagtttgg ttaagtcacg ggcggttgaa acgttatgca ccgacaatcc ggttggttca
    15721 acaaagcgcg tgttgttcat tccgagcgat ttcgctttcg cattcatcgc cttaataaag
    15781 gctttgtaac caccgggata atggtgcgca aggcttgccg ccgcgcggtt ttctgaagac
    15841 atcagcgcca gcaacagcat atctttacgg ctgatttcgc tgttcagtcg tacgcgcgaa
    15901 tagacccctt tcatctccgg cgtctggctg atatccactt ttagtttttc atccagcggc
    15961 agtcgtgcat ccagcacaac catcgcggtc attaatttgc tgatagacgc aatcggacgc
    16021 accagatccg ggtggttcga atagatcact ttgttggtat tcagatcaac aatcatcgcg
    16081 ctaccggagg caatttccgg ttgtgacgcg gtggtagcgg ctgccgtttt cgcaacggcc
    16141 tgcggtgcaa aaggcacagt cagcatcagg gccaggctaa ataaagaaac tcgaaatttc
    16201 ggcatgatga gcattcagat agtggttcac gcgcacgggt tgcgcaccgc cggagtaagg
    16261 atttactgag gctagcgacg ccatcataac gagcaaaaag tgcgatcgtc aaagaagaat
    16321 cgtgaggaaa tgctgcattg ctgacattta cgccagcaat gcagcgcaaa agaactttct
    16381 agaacaaacg ataaccgtag ccccatagta taacggtaag ggcgagcagc acttccagca
    16441 ccagcacgcc tatcgccagt gtcgaactgg agaagctaag tccttcctct ttgttgatat
    16501 tcaggaagct cggaataccg agatacagca ggtagccggt gtaaaacagc gccacagtgc
    16561 cgaccagcgc acacaaccag accagcgggt aaagcgccac cagaccgctt aaaaacagcg
    16621 gagttgcaac gtagcccgcg aagaccatgc agtgcgcaag tgacggacgc tgcggataat
    16681 tacgcgccat ccaccagatg acccgcccca tcaccgcaac cccagccagc ataacgccgt
    16741 aaaacaagac agccagcgcc agtccggtaa accaggataa cttcaggata gtgccatcgc
    16801 caaaattcca gccaatctgt gtagtgccaa tgaaggcgca aatcaccgga atcgccgcca
    16861 tcagcaaaac gtggtgggtg tagtgatgag aaatcgtttc gttttcgcga ttaatcacct
    16921 gcatttcacg atcgggatgg gaaaacagtc cccagacatg gctcataccg cccccttgtt
    16981 gtgagttcat gaacctgaca gttcaagtat aagtcagctt gtgattattt tttgttcgct
    17041 catgtaattt gcaccgtctg atgtacgtcc tttttcagcg tgaatgattc acagggtgta
    17101 tgcttacagt caagcaaagg gagacagact gtcctatgga tctcaataca ctcatctcac
    17161 aatatggtta tgccgcgctg gtgatcggta gcctggcgga aggtgaaacc gtgactttgc
    17221 tgggaggcgt tgcggcacat caggggctat taaagttccc gctggtggta ctttctgtgg
    17281 cgcttggcgg catgattggc gaccaggtgc tttatctttg cgggcggcgg tttggcggca
    17341 agctgttacg ccgtttctcg aaacatcagg ataaaattga gcgggcgcag aaacttatcc
    17401 aacgccatcc gtatctgttt gtcattggta cgcgttttat gtatggcttt cgggtcattg
    17461 gcccgacgct gattggtgcc agccagctgc cgccgaaaat ctttctgccg ctgaatattc
    17521 tcggcgcatt tgcctggtcg ctgattttta ccactatcgg ttacgctggt gggcaggtga
    17581 ttgcgccgtg gttgcacaat ctcgaccagc atttgaagca ctgggtctgg ttgattctgg
    17641 tcgtggttct ggtggtaggc gtgcgctggt ggctgaaacg gcgcgggaag aaaaagccgg
    17701 atcatcaggc gtaaaactat tgccctggat aaggtgttcg cgccgcatcc gacatccggt
    17761 gcaccatgcc tgatgcgacg ctggcgtgtc ttttctggcc taacccaatg aaataaccac
    17821 gcacctcact gcgctattct gggttgaact gcggattcac caacataaag ccgccatcca
    17881 ctatcagcga ctgcccggtg gtgtaatttg cgccctccga acaaagccat accaccaggc
    17941 tggcaatctc atgcgttgtg ccaaaacgtc gcaagggaat cgaaggctcc gcgtcgggct
    18001 tcacgtcgcc gccatccatg ccgttcattg gcgtggcgat cgccccaggt gcgactgcgt
    18061 tcaccaaaat tttatgcctg accaactcca gcgccattgc tttggttaac ccgccgagcg
    18121 catgtttagc ggctgtgtag gcgctggcat caggcagcgg cgtatgttca tgtaccgatg
    18181 taatgttgat gatgcgaccg ccctgccctt gcttcaccat ctggcgggca gcaatttgcg
    18241 agcataaaaa tgcaccatcg acatcaacgg taaaaatctt gcgccactca tcgaaagcca
    18301 tatcaagaaa cggcgctttg gtcattgcgc ccgcattatt caccagcaca tcaatacgcc
    18361 ccagccgttg aataagtttc tccagcgcct gcgcaccttc tggtagattg ccgagatcca
    18421 gttgcacgat ctccgcacgt acgccgtggc taactacctc acgcgcggta tcttttgccc
    18481 cttcttcatc tgagtgccag gtaatgccaa tatcaaaccc ctgctgcgcc agtaataacg
    18541 cgcactcttt gccgatcccc gaatcggagg cggtaattat cgcgacctgt gccatcgagt
    18601 tctccactta acgctcaaca aacgttaagt atagaaggcg catatcatca gcgtttgtac
    18661 cccccaccca gcgcaccagt gagttgaatg gaggcatcca gccactgccc ttgcaataac
    18721 aggccatggg cccgctcacg cagcgccggg attctggctt cgctgacccg ggaaccagca
    18781 atgaggcccg cgttaaagcg cgcctgcgca agacctacta cacgcaaggc atcgcgctca
    18841 atttgcgcct gatgctggtt tttctccgcc agtgtctgaa cctgactggc tgcccgcgcc
    18901 acgtcattca ccgcttcaac caccgctttg ttgtagctgg cgatagacaa gttgctttcg
    18961 gcttttgcga tatcgagg
//
LOCUS       DAZFFE010000092        18897 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFFE010000092 DAZFFE010000000
VERSION     DAZFFE010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.092"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..726)
                     /gene="glxR"
                     /locus_tag="ACHJ69_003524"
     CDS             complement(<1..726)
                     /gene="glxR"
                     /locus_tag="ACHJ69_003524"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO5016432.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKLLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLA"
     gene            complement(826..1602)
                     /gene="hyi"
                     /locus_tag="ACHJ69_003525"
     CDS             complement(826..1602)
                     /gene="hyi"
                     /locus_tag="ACHJ69_003525"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO5016433.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPTGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(1615..3396)
                     /gene="gcl"
                     /locus_tag="ACHJ69_003526"
     CDS             complement(1615..3396)
                     /gene="gcl"
                     /locus_tag="ACHJ69_003526"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO5016434.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKILMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(3486..4301)
                     /gene="allR"
                     /locus_tag="ACHJ69_003527"
     CDS             complement(3486..4301)
                     /gene="allR"
                     /locus_tag="ACHJ69_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO5016435.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHL"
     gene            complement(4379..4861)
                     /gene="allA"
                     /locus_tag="ACHJ69_003528"
     CDS             complement(4379..4861)
                     /gene="allA"
                     /locus_tag="ACHJ69_003528"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO5016436.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQKRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            5091..6017
                     /gene="allS"
                     /locus_tag="ACHJ69_003529"
     CDS             5091..6017
                     /gene="allS"
                     /locus_tag="ACHJ69_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO5016437.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTATGEHLLCQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVARLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMLDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            6086..7180
                     /gene="mnmH"
                     /locus_tag="ACHJ69_003530"
     CDS             6086..7180
                     /gene="mnmH"
                     /locus_tag="ACHJ69_003530"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO5016438.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGICYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPHGILCCARGGQ
                     RSHIVQRWLHDAGIDYPLVEGGYKALRQTAIQATIELSQKPIVLIGGCTGCGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQATIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVVEWVKAQ"
     gene            complement(7421..9835)
                     /gene="ybbP"
                     /locus_tag="ACHJ69_003531"
     CDS             complement(7421..9835)
                     /gene="ybbP"
                     /locus_tag="ACHJ69_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO5016439.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLMAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAITGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLHNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLAFLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNGDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQSKVFDFPWEPDWRLWIVLPCSGALLLSLCGGWLGARLLKGKAL
                     FRQFAG"
     gene            complement(9832..10518)
                     /gene="ybbA"
                     /locus_tag="ACHJ69_003532"
     CDS             complement(9832..10518)
                     /gene="ybbA"
                     /locus_tag="ACHJ69_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO5016440.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            10489..11112
                     /gene="tesA"
                     /locus_tag="ACHJ69_003533"
     CDS             10489..11112
                     /gene="tesA"
                     /locus_tag="ACHJ69_003533"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO5016441.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            11102..11911
                     /gene="ybbO"
                     /locus_tag="ACHJ69_003534"
     CDS             11102..11911
                     /gene="ybbO"
                     /locus_tag="ACHJ69_003534"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO5016442.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVGKVRHAFISEKPKMRYPVTLVAWAVMVLKRLLPGRVMDKILQG"
     gene            11972..12826
                     /gene="cnoX"
                     /locus_tag="ACHJ69_003535"
     CDS             11972..12826
                     /gene="cnoX"
                     /locus_tag="ACHJ69_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO5016443.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGLFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(12889..13668)
                     /gene="fetB"
                     /locus_tag="ACHJ69_003536"
     CDS             complement(12889..13668)
                     /gene="fetB"
                     /locus_tag="ACHJ69_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO5016444.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13655..14332)
                     /gene="fetA"
                     /locus_tag="ACHJ69_003537"
     CDS             complement(13655..14332)
                     /gene="fetA"
                     /locus_tag="ACHJ69_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO5016445.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLCAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            14478..15395
                     /gene="qmcA"
                     /locus_tag="ACHJ69_003538"
     CDS             14478..15395
                     /gene="qmcA"
                     /locus_tag="ACHJ69_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO5016446.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLHIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            15392..15850
                     /gene="ybbJ"
                     /locus_tag="ACHJ69_003539"
     CDS             15392..15850
                     /gene="ybbJ"
                     /locus_tag="ACHJ69_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO5016447.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLLPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGTGTHVEVIAIEGITLIIRAVIA"
     gene            15969..17192
                     /locus_tag="ACHJ69_003540"
     CDS             15969..17192
                     /locus_tag="ACHJ69_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016448.1"
                     /translation="MFSPFRKPEVRLLKNTAAGVIFSVKSGKGFLRQCVIYDPHSHGL
                     IHSLCWQDVPLIRFWSEAGCPTCAEFVYSGFAEDEQGAARFLSALENWNRPWSGITDA
                     FAALTPPFSCMADGYYLLEDRELYPTDGNGHFFWAATDHSSSNPATVAVWDPEYCSFS
                     DTAPCFLLPGQPPSHFNPERATFYRDKPDARALAWYLPDSYLCVLLDGHHKATAAALE
                     GRPLKTLVLSTATHFNDEQQTLVFPGGECLHKTELQYHIPKLTACKTLPPSAWESFGP
                     DKHISPSETWSEELKQSVSRYPSLDQAWQIVEAGNLSETRIKSMIQQGLGEDEKADII
                     LQALFSTHSPLFIDFARFVISHPAYAIYRPLTFRLMAQNRTPQADAFFLDFAINDDGE
                     RPELTKIMDDYFRKP"
     gene            complement(17193..17600)
                     /gene="cueR"
                     /locus_tag="ACHJ69_003541"
     CDS             complement(17193..17600)
                     /gene="cueR"
                     /locus_tag="ACHJ69_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO5016449.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIEQHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(17597..17694)
                     /locus_tag="ACHJ69_003542"
                     /pseudo
     CDS             complement(17597..17694)
                     /locus_tag="ACHJ69_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17725..>18897)
                     /gene="ybaT"
                     /locus_tag="ACHJ69_003543"
     CDS             complement(17725..>18897)
                     /gene="ybaT"
                     /locus_tag="ACHJ69_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO5016450.1"
                     /translation="LMEASTWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRG
                     LGNGVFSLALSLLYLLTLAVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVM
                     TLFNSLSNHAVGRLEVILVGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIG
                     ITFLAYAGFGMMANAADKVKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELE
                     KYADTAVAQAASPLLGHVGYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKL
                     MNKSLWRQSTWGNIIVVVLIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDI
                     HASLPILIVGTLVMLLVIVGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
BASE COUNT         4629 a         5167 c         4764 g         4337 t
ORIGIN      
        1 tgccaggttg agatctttct ggtgcagcgc aattttaaaa cctggattaa aggtgcgttt
       61 aatcatgcgc tcaccgtgaa cttccagaat acgtgaggaa gcaaagccgc ccatcagcgc
      121 ctggcgcaca cgtaccggat ccgctccggc ttttgaagca aatagcaggg cttcagaaac
      181 cgcttcaata ttgagcgcta cgataatctg atttgctact ttgcaggttt gaccgtcgcc
      241 gttaccgccc acgagggtga tatttttacc gagcagttca aacagcagtt taacacgttc
      301 aaataccgct tcgtcaccgc caaccataat cgacaacgtc ccttcacgcg cgccgatttc
      361 accgccggag acgggcgcat cgagataatc accgcccagt tcattcacct gacgagcgaa
      421 acgtttagtt tcgatcgggg aaatggagct catatcaaca atggttttgc ccttcaacga
      481 ggctttggta caaccatttt caccaaacag aacttcttca acctgtggcg tatccggcac
      541 cataataaaa atgatgtccg atgcttccgt tacctggcgc gcagtttcaa cactgacggc
      601 acccagtgac agtaattcat cagcaaccgg accaatggtt gtgacatgca gttgatgacc
      661 ggcacgcgcc agattaatgg ccatcggtgt acccataatg cctaagccaa taaatcccag
      721 tttcataatt aacctctttt aaatttcgct tttcctgaat tcagacaaca cgatcccctg
      781 ccgacttagc gacctaaaaa agcattgtct gaatagcgtt acgttttaac gatacggatc
      841 catccagcgt aaaccggctt ccgtggtggt ttggggttta tattcacacc caacccaacc
      901 gttgtagtca gaattttcaa ttaccgtaaa gagatactcg taattaattt ctccggttcc
      961 cggttcgccg cgatgcggat tatcggcaat ttgcaggtga ccaattttat cggcccattg
     1021 agtcatagtg ttggttaatt caccttccat ccgctgcata tgataaatgt catactgaat
     1081 tttcaaattg cagcaaccaa catcatcaat cagtttcagc gcctgccgag ttccggtgag
     1141 atggaaacca ggaatatcaa aatggttgat aggttcaatc agtaataaga tatcttcttt
     1201 catcagcata ttcgcggcat aacgcaggtt ttctacaagc gttgcgtgaa tctgttcact
     1261 gctgaaacca gtcggcgttt taccgaccag acagttaatt tttttattac ccagcgcacg
     1321 agcataacga atcgctgctg ctacgccatc ccgaaactct tcttcacggc caggaataca
     1381 agcaataccc cgctcgcccg ccgcccagtc acctgccggt aaattgtgca gcgtatgttc
     1441 gagtttatta ctcgccagca cctgttttaa ttcttcaatg tcgtagtcat aagggaacat
     1501 aaattcaacg ccgcgaaaac cacactgcgc agctttctca aaacgggcga gaaaatcata
     1561 ttcaccaaat aacatcgata aattagcaga gaaacgtaac attatttatc tcccttattc
     1621 atagtgcatg aaacaggttt cagtcggtgc gtccgcggcg ttatcggcga tatcttcaaa
     1681 ttccatgacg ttatccagtt cactgcccat cgaaatattg gtcacacgct cgagaataac
     1741 ttccacgact accggtaccc gatattgcgc cattaagatt ttcgcctgtt caaaggctgg
     1801 tgcaatatct tccggtttga aaacgcgaat tgctttacaa cctaaacctt ccgctacttt
     1861 tacgtggtcg acgccgtaac cgttcacttc gctggagtta atattttcga acgcgagctg
     1921 tacgcagtaa tccatatcaa acgcgcgctg cgactggcga atcagaccca gataggcgtt
     1981 attgaccagt acatggatgt acggaatgtt gaactgtgcg ccaacggcta attcttcaat
     2041 caggaactgg aagtcgaagt caccggaaat cgccaccaca ttgcgctctg gatcagcggc
     2101 acacacgccc agcgccgccg gaatggtcca gcccaacggg cctgcctgcc cacagttgat
     2161 ccagtggcgg tctttaaaga catgcagcat ttgtgccgca gcgatttgtg acagaccaat
     2221 ggtggtgaca taacaaacat cgcgaccaaa ggctttgttc atctcttcat acacgcgctg
     2281 cggtttcacc ggcacattgt cgaaatgggt tttgcgcagc aaagtgcgtt tgcgctgctg
     2341 gcagtcggcg acccattctt tacgacacgg cagacgaccc gctttttgca tctcctgcgc
     2401 cacttcaacc agcagcgtca gcgccgcttt agcatcagag acaatgccga gatccggaca
     2461 cagcacgcgg ccaatttgcg tcggctcaat atcgatatga acgattttgc gcccttcggt
     2521 gtatttctct accgaaccgg tatgacggtt agcaaaacgg ttaccgatac caaacaccat
     2581 gtcggacgcc agcaacgttg cattaccgta acgatgcgcg gtttgcaggc ccaccatccc
     2641 ggccatcagt tcatgatcgt ccgggataca gccccagccc atcagcgttg ggatcaccgg
     2701 aacgctggtc agttcagcaa actgttgtaa cagtgcagct gcgtcagcat tgattacccc
     2761 gcctccggca acaatcaccg gacgttcggc ctggattaac atttctacag ctttttcgat
     2821 ctgcatacgg ctggcagcag gtttgtagac cggcagcggt tcgtacatat caggatcaaa
     2881 ctcgatttcc gcaacctgta cgtcgaacgg taaatccacc agtaccggac ccgggcgacc
     2941 agaacgcatc aggtgaaacg cctgttgcag cacacgaggc accagcgccg cttcacgaac
     3001 tgtaaccgcc attttgctga ccggtttagc aattgcttca atatcaacgg cctggaaatc
     3061 ttctttatgc agacgggcgc gcggagcctg gccggtaata cacagaatag gaatggaatc
     3121 agcagaagcg gaatagagcg cggtgatcat gtccgtgccc gcaggaccgg aagtccccag
     3181 acatacgccg atattccctg ccgttgcgcg ggtataacct tccgccatgt gcgaagcacc
     3241 ttccacatga cgcgccagaa tgtgacgaat accgccgtgc ttacgcatcg ctgagtagaa
     3301 cggattgatt gcagctcccg gaacgccgaa ggcggtggtg ataccttctt tctccagcac
     3361 atacattgcc gcgtcaacgg ctctcatttt tgccatttta ttcctacctc tattaattgg
     3421 aaaaattttc caactttcaa attttgtcaa aaaaacccgc tccgcagagc gggatacgac
     3481 agacactata gatgtgcttt cagtcccaac gccgtgctga tatcgcgggc ggtgtctctg
     3541 accagctcac cctgactgac aaaacgatct tctgtcagtc ttgatgaagg cccggagatg
     3601 gagatagcgg caacaacact acctacatca tcgtaaattg ctgaagctat gcaattcagg
     3661 ccgacaacat gctcttcttt atctacggta tagcccagtt cacgcgcttg ttccaggtcc
     3721 ttcagcaagg tgggcatatc cacaagtgtc gttggcgtaa actgctgcaa accggtttgc
     3781 agaatgatgc tcatcaactc ttcttccgcc agcggataaa gcagcgcctt gcccgctccg
     3841 gaagcatgca gcggcagacg actgcccagc ggcgcacaca tcctgaccat cgatttacac
     3901 tctaactgac caattaatac cgcttcattg ccgttacgga tcgcgacatt gaccgtttcg
     3961 ccggaaagta acatcaggcg gcgcataaac ggcccggcga cggagagtac atcgcggtta
     4021 tggatgtacg ctgcaccgac gttaaagaca cctaatccta tatgccacca gcctaactga
     4081 ctgtcctgat agacaaaatc cgctgcctgt aaaaccttca gcaagcgaaa ggtcgtggag
     4141 agcggcaaat ccagattgag agagatatcg ctaaccgacg aacttccccc acttttttcc
     4201 agatattgca gaatcgcaat tccccgctct aacgcctgtg cacccttctg tgccacaggc
     4261 tccgcctgtc ctggcctgcc gcgccgtcta acttccgtca tacctacgtt aactccgacc
     4321 tacaaatgag cataaacaat gaacaattcg tttatcttat gcaagtcggt tgcaggcgtc
     4381 acgcaaaaca gagttcctgt tcaggaatac tttcaacatc acagttgtca ctgccgccgc
     4441 gatcgatggt cagaaaatcg gtgacgcgct gccaggcgaa aagtgggtga tgccagacgt
     4501 tacgatggta attcactccc tgttcgccat tggtaataaa cgcccgcagc gttgacaggt
     4561 ctggtttgtc gtcgcctaac gccacgacca ccacaaacac ttcacctttc atcgggataa
     4621 aggcctgagt gcccaacgga tgacgttcga gttcgtgaat ggtcagcggc agattcgccg
     4681 gttgcgcgcg gttaatgctg ataagcgtac ggtcttgctc aagaatctca accagcgcca
     4741 aatcgtggta acgctccacc agtccattgt taatatggaa aaaatcccgt ttctgcgttt
     4801 cgattacgtc gccataagca ctaaaggctt cctgacttaa cggtaatacc tgaagtttca
     4861 tctttttgtc cttgtaacat aattccgttc aaccaccgcc gaccggtcag cttggtttca
     4921 ataacaggca atctaaaacc ttaagagaac aacatcaaca caaaagttgg aatatttttc
     4981 caaaactaga tttagtccac aaattgaaca gggttatcat gtaaaaatac tgataaatca
     5041 gaggataaga aaatcacgca acttgcggat ttaaaagggg atacctgaag atgttcgatc
     5101 cagaaacctt gcggactttc attgcggttg ctgaaacagg tagtttttca aaagcggcag
     5161 aacgattatg taaaaccacg gcgacgatca gttatcgcat taaacttctg gaagagaata
     5221 ccggagtggc gctgtttttc cgcacgactc gcagcgtgac gttgacggcg actggcgagc
     5281 atctactttg tcaggccaga gactggctga gctggctgga aagtatgccc agcgagttac
     5341 aacaggtgaa tgatggcgtg gaacgccagg tgaatatcgt catcaacaac ctgctctata
     5401 acccccaggc cgttgccagg ctgctggcgt ggctaaatga acgttacccg tttacccaat
     5461 ttcacatctc ccgacaaatc tatatgggcg tctgggactc gctattgtac gaaggttttt
     5521 cactggctat cggcgtcacg ggaaccgagg cgctggcaaa tacctttagt cttgatccct
     5581 taggatcggt gcaatggcgc tttgtcatgg cggcggatca tccgctggcg aacgttgaag
     5641 agccgctaac agaagcacag ttgcggcgct ttccggcggt caatattgaa gacagcgccc
     5701 gcaccttaac caaacgcgtc gcctggcgat tgccggggca aaaagagatt attgttcccg
     5761 atatggaaac gaaaatcgcc gcccatctgg cgggcgttgg cattggtttt ttgccaaaat
     5821 cactttgcca gtcaatgctc gataatcaac aactggtcag ccgggtaatc ccaacgatgc
     5881 gccctccttc gccattgagt ctggcatggc gcaaatttgg cagcggcaaa gcggtagaag
     5941 atattgtgac cttgtttacc cagcgcaggc cggaaatcag cggattttta gaaattttcg
     6001 gcaacccacg cagttaaaaa tcaagtttcc ttgcgcgttg gcgcgtaaaa tagcgcgcaa
     6061 gctgatttga ggttcacttt ttactatgca agagagacac acggaacagg actatcgtgc
     6121 cctgctgatt gctgatacgc ccattattga tgttcgcgcc cccatcgagt ttgagcaagg
     6181 cgcaatgccc gccgctatca acctgccgtt aatgaataac gatgaacgcg ccgccgttgg
     6241 catctgctat aaacagcaag ggtcagacgc ggcgctggca ctggggcata aactggtggc
     6301 gggtgaaatt cgtcagcagc gcatggacgc ctggcgggca gcgtgcctgc aaaatccgca
     6361 cggtattctc tgctgcgccc gtggcggtca gcgctcgcat attgtgcaac gctggctgca
     6421 tgacgcgggg attgattatc cgctggtgga aggcggttat aaggcactgc gccagaccgc
     6481 gattcaggcg accattgaac tgtcacaaaa accgatagtg ctgattggcg gttgtaccgg
     6541 ctgcggcaaa acgctgttag tgcaacaaca accgaacggt gttgatctgg aagggctggc
     6601 gcgccatcgc ggttctgctt ttggtcgcac gttacaacca caacttagcc aggcgagttt
     6661 tgaaaacctg ctggctgccg aaatgctaaa aaccgacgcc cgtcagaatt tgcgcttgtg
     6721 ggtgctggaa gatgaaagcc ggatgatcgg ttcgaatcac ctgccggaat gtctgcgcga
     6781 acgaatgact caggcgacga ttgcggtggt agaagatccg tttgagatcc gtcttgagcg
     6841 gctgaacgaa gaatatttcc tgcgtatgca tcatgatttt acccacgcgt acggcgacga
     6901 acagggctgg caggagtatt gcgaatacct gcatcacgga ctttcggcga ttaagcgtcg
     6961 gctggggcta cagcgctata acgaactggc tgcaaggctg gatgcggcgc tgacaacgca
     7021 actcaccacc ggcagcaccg acggtcatct ggcctggctg gtgccgttac ttgaagaata
     7081 ttacgacccg atgtatcgct atcagcttga gaaaaaagcg gaaaaagttg tctttcgcgg
     7141 tgagtgggcg gaagtggtgg aatgggttaa ggcgcagtga tgggggattc tgtgcacgct
     7201 gttcatgccg gatgcggcgt gaacgcctta tccagcctac aggtgagtgc tgtttcgtag
     7261 gcatgataag acgcgcctgc gtcgcatctg gcatcggcgc acaactgccg gatgcggcgt
     7321 gaacgcctta tccggcctac aaaatttcga aaactcaata tattgcacga tcttcgtagg
     7381 cctgataagc gtagcgcatc aggcaatcta acacctgtca tcaccccgca aactgcctga
     7441 acagcgcctt acccttaagc agtcgcgcac ccagccagcc gccgcaaagc gacagcagta
     7501 gcgcaccgct gcacggcaga acaatccaca atcgccagtc tggctcccac gggaaatcaa
     7561 acactttcga ttgcagtacc gccagtgccg tttccgcacc aattgcggcc accaggccgg
     7621 atacaaaccc gagcatggcg aactcacacc acaatgtcgt gcgcagcagt tttttgcccg
     7681 caccgagagt gcgccacacc accagctcct gatgacgctg acgcattccc acctgcacct
     7741 gtgccagcaa caacaacata ccgcaggcgg tgaccagcac caccatcact tccagcgccc
     7801 gacttacctg ctccagcacc tgaccgacct gttttaaaat cgcgccaatg tctaacaggc
     7861 taatggtcgg gaactggcgg ttgagttgcg tcaacatgcc gttgccattc tcccagcgga
     7921 aactggtaag ccagctctgc ggttgcccgt ctaatgcccc ttccgggaaa ataaaataga
     7981 aattaggccg cagactttcc cagtccactt tgcgcaggct ggtcacttta gcgcggaact
     8041 cctgggtatc gcccataaaa gtcacggtat cgccgagggc aacgtttaag cgtttcgcca
     8101 gcccctcttc catcgacact tcatcggctt ttggcggcca gttaccggcg acgatcgggt
     8161 tatgatcagg ccgcgtattt tgccaggtga gattcagctc gcggttgagc gcctcatctc
     8221 cattgccttc tgtcggcttg tcgttaatcg ccgtcaaccg cgcccgcacc accggataaa
     8281 acgattccgg gactatctga tgttccgcga gaaacgcttt cagcggcgta acctgttccg
     8341 tagcgatgtt gattaaaaag tagttcgggc tttctggcgg cagttgttgt tgccaacggt
     8401 cgagcagatc gccacgcaac accagcaaca gtgccagcag cataaaggag agcgaaaatg
     8461 ccgaaagctg gcttaacgtt gaccacggct gacgtaacag gcggctaacc gccaggcgca
     8521 gaggtagcga tttcagcgtc atgcggcgaa gtacattcag cagcatccag cccagaacac
     8581 cgcacagaaa agccagtact accgcgcccg ccagcaccgc ccaaagcagc atactgccgc
     8641 ccattaaccc ggcaagcagc agcacaacca ccacactgac aatcggcaga taaaacttga
     8701 gcggccagac gttcgcgacc acatcattat gtaatacgcg taaaggctgc gtggccagta
     8761 acagtcggta cggtcgtagc cccaccagca gcgagatgac tgtcatggtg ccgagcgccc
     8821 acagccacgg ccagaggctg gcaggaggta aagcggcagg tagcacgggc ttgagcagca
     8881 ccatcaacac gttttcgaac aacaggccta tcgccccacc ggtaatggct gaaagcgtca
     8941 gcaccatcaa ccactgaccg acgattagct tacgcagttg cgctcgccct gcccccagcg
     9001 ttttgaggat cgccatcaga tcgtagcgac tgcgacagta atgattcatc gccaccgcca
     9061 ccgctgccac tgccagcagc aaggtcagaa gcgcagaaag cagcaggaac tgttgcgagc
     9121 gttccatcga gcgccccagc gcgccttcgt cctgttccag accgtaccag cgttgttcgg
     9181 gtttaagctg gggtaacaac catttctcat agccgtcgag ctggttctcg ttgccgccga
     9241 atttataacg ccaggtgacc cgactccccg gctgcacggc tccggtttta tcgacatccg
     9301 ccagattcat catcagacgc ggagccatct ggaaggggtt aaaaccggaa tccggttcct
     9361 gaatcacttc tccggcaatc cgcaaggtgg catcgcccac gtcaatggtg tcgcccgttt
     9421 tcaggttaag cagtgccatc aggcgtgggg ccagcaatac gctgcccgcc tgcggtttca
     9481 ggcctggggg attggtttgc agatcgccat acatcgggta gatatcatcc accgctttga
     9541 cgttcgccag ctgcggtgtg tcgcctgcaa atgtcatggt ggcgaaagtc agctgcttgc
     9601 cgactttcag gccgcgcttt tgcgcttcct ccagccacgc ttgcggcact tcgcgcgaac
     9661 tacgcaacgc ccgatcgccc gccataaact cccggctttg ctggcttaag cccttctcca
     9721 tgcgatcgct gatattgccc agcgccagca cgcaggccac cgccaggctt agcgccagcc
     9781 agacaattaa tagcgacggc gagcgccatt cgcgccagaa ccaacgtgca atcatgcttc
     9841 ctcctgcaac tgcccgttaa ccagtcgtaa gcagcggtcg cagcgtgccg ccagttgcag
     9901 gtcgtgggtc accataatca acgtggtgcc atgttcacgg ttgagggaaa acagcaggtc
     9961 ggcaatttta tcgcccgtct ggcggtcaag attgccggtg ggttcgtcgg caaacagcac
    10021 atcaggccga ccattaaaag ctcgcgccag cgccactcgt tgctgttcgc cgccggaaag
    10081 ctgtgccgga agatgatcca gacgtttacc cagtcctaac tgttcgagca acgcttttgc
    10141 cccgttacga ctttctgcgc tactctcacc acgcagcagc gccggaagct cgacgttttc
    10201 cagcgcgtta agggtaggaa ttaacataaa tgactgaaaa acaaagccta cgtgcttcgc
    10261 gcgcaacttt gcccgcgctt cttcgtccat attatgtagc ggttgtccca ccagactcac
    10321 ttcgccactg ctgccgtcat ccagccctgc gaggatcgcc agcaaggttg acttacccga
    10381 tcccgactcg cccaccagtg caatggtctc accacgtttg acaaccagct caactccggt
    10441 gaggatggag agttcatgct ccccctgacc gacggacttc ttaagatgat gaacttcaac
    10501 aatgttttcc gctggcattt gcccttcctg ttcctggtcc tgttaacctt ccgtgccgcc
    10561 gcagcggaca cgttattgat tctgggtgat agcctgagcg ccgggtatcg aatgtctgct
    10621 agcgcggcct ggcctgcctt gttgaatgat aagtggcaga gtaaaacgtc ggtagtcaat
    10681 gccagcatca gcggcgacac ctcgcaacaa gggctggcgc gccttccggc tctgctgaaa
    10741 cagcatcagc cgcgctgggt gctggtagaa ctgggcggca atgacggttt gcgtggtttt
    10801 cagccacaac aaaccgagca aacgctgcgc cagatattgc aggatgtcaa agccgccaac
    10861 gctgaaccat tgttaatgca aatacgtctg cctgcaaact atggtcgccg ttataatgaa
    10921 gcctttagcg ccatttaccc caaactcgcc aaagagtttg atgttccgct gctgcccttt
    10981 tttatggaag aggtctacct caaaccacaa tggatgcagg atgacggtat tcatcccaac
    11041 cgcgacgccc agccgtttat tgccgactgg atggcgaagc agttgcagcc tttagtaaat
    11101 catgactcat aaagcaacgg agatcctgac aggtaaagtt atgcaaaaat cggtcttaat
    11161 taccggatgt tccagtggaa ttggcctgga aagcgcgctc gaattaaaac gccagggttt
    11221 tcatgtgctg gcaggttgcc ggaaaccgga tgatgttgag cgcatgaaca gcatgggatt
    11281 caccggcgtg ttgatcgatc tggattcgcc agaaagtgtt gatcgcgcag ccgacgaagt
    11341 gatcgccctg accgataatt gtctgtatgg gatctttaac aatgccggat tcggcatgta
    11401 tggccccctt tccaccatca gccgcgcgca gatggaacag cagttttccg ccaacttttt
    11461 cggcgcacac cagctcacca tgcgcctgtt acccgcgatg ttgccgcacg gtgaagggcg
    11521 tattgtgatg acctcatcgg tgatgggatt aatctccaca ccgggtcgtg gcgcttacgc
    11581 ggccagtaaa tatgcgctgg aggcgtggtc agacgcactg cgcatggagc tgcgccacag
    11641 tggaattaaa gtcagcctga tcgaacctgg tcccattcgt actcgcttca ccgacaacgt
    11701 caaccagacg caaagtgata aaccagtcga aaatcccggc atcgccgccc gctttacgtt
    11761 gggaccggaa gcggtggtcg gcaaagtgcg ccatgctttt attagcgaga agccgaagat
    11821 gcgctatccg gtaacgctgg tggcctgggc ggtaatggtg cttaagcgcc tgctgccggg
    11881 gcgcgtgatg gacaaaatat tgcaggggtg agttgaagcg cgcgcttaag cccccatgtc
    11941 aaagaaaata tcgacaacag agagcgactc catgtccgta gaaaatattg tcaacattaa
    12001 cgaatctaac ctgcaacagg ttcttgaaca gtcgatgacc actccggtgc tgttctattt
    12061 ttggtctgaa cgtagccagc actgtttgca gttaacccca attctggaaa gcctcgcggc
    12121 gcagtacaac gggctattta ttctggcgaa gctggactgc gacgcggagc agatgattgc
    12181 cgcccagttt ggtctgcgcg cgattccgac cgtatatctg ttccagaacg ggcaaccagt
    12241 agacggcttc caggggccgc aaccggaaga ggcgatccgc gccctgctgg ataaagtgct
    12301 gccgcgtgaa gaagagctga aagcgcagca ggcgatgcaa ctgatgcagg aaggcaatta
    12361 caccgacgct ctgccattgc tgaaagacgc ctggcagttg tcgaatcaga acggggaaat
    12421 cggcctgttg ttggcagaaa cgctgattgc gctgaaccgt tctgaagatg cagaagccgt
    12481 gctgaaaacc attccgttgc aggatcagga cacccgctat caggggctgg tggcgcaaat
    12541 cgaactgctg aagcaggcgg ctgatacgcc ggaaattcaa cagttgcaac agcaggtggc
    12601 ggagaatcca gaagatgccg cactggcgac gcaactggca ctgcaactgc accaggttgg
    12661 gcgcaatgaa gaggcgctgg agttactgtt tgggcatctg cgtaaagatc tcaccgccgc
    12721 agatggtcag acacgtaaaa cattccagga gatcctcgct gcgctgggta cgggtgatgc
    12781 actggcgtcg aagtatcgcc gccagctgta tgcgttgttg tattgatgtt ttgttgatcg
    12841 gcacatgaac cacaacgccg gatgcgatgc tgcatgtccg gcatcgcatc atttcttctt
    12901 caattgcgtc accaccaatt ggtggcgcga attgtaaaac ttacgatagg ttaaatagca
    12961 ggcaataatg gtcgacaagc tggcggttga gagcagcata aaggtcacca taatctgata
    13021 tttaatcgcc ttcaccggat caatcccggc aaatatcagc ccggacatca ttcctggtaa
    13081 actcactaag cctaccgttt ttgctgaatc gaccgtcgga attaaagccg cgcgaatact
    13141 gtcgcgaatc aatatcgctg aagcctgttt cggcgtcgcg ccaagactca gtttctcctg
    13201 aatctgttgc tgttcgctaa tgaccctttg ccctaaattg ttgtaacaca accccaccgc
    13261 taccatggcg ttgccggcta tcatcccggc gatagggatc acctgcatcg ggataaattc
    13321 aatcgacccg gagaggatca gtaccgccag ggtaattccc gccccgaccg taatggcaat
    13381 aaacgatgag ataaaagctt tagcaatata tttactgcgt ttttgcgcgt tccacgccgc
    13441 attaaagcag ataaataaca ccatcaataa tgtcaggctg gcatcatcca cgctgaaaat
    13501 atacttcagc acatagccga caataatcag ctggattatc gcgcgcccga cgctccagag
    13561 aatatctttc tccagcgcca gtttttcttt atggctaatt aagattgcca ccaccaccag
    13621 cattaatgcc agtgctaatg attcgttagt aatattatgc gagttcatag cgtgcttcct
    13681 gcatttctcc agcatgcggt tgcagtgtaa tcactttatc cgcatgatta atttcgtctt
    13741 tatcgtgtgt cacccacagc acagcaatat tttgctcgcg tacataacgg tggatcatct
    13801 cattaacgtt atgtttatta ctttcatcca gcgcactggt gatttcatcc agcaataaaa
    13861 ccttcggcat aaattgcagg ttacgaatca atgagatgcg ttgtttttca ccaccagata
    13921 gctcggcgat attcttcgtc aaaatgctgt ccggtaaagc gaagcgttca agaaaatcga
    13981 gaaaaatggc cgggtcaggc tgctggttac ggatctgcca gggaaaaatc aggttatcgt
    14041 ataccgtatc gccaaacagc gtcggcgtct gggcgcagta agagacttgc tgacggtaga
    14101 tttctggctt tagtgtgctg acatcctcac cttcaaacag taacgttccg ctggttgggc
    14161 tgatcaatga agcaactatt tttagcagtg tacttttgcc acaaccagaa ggaccggtaa
    14221 ttaacttaaa ttcgccagca cacagcgaaa aattgatgtt attaagaatc ttcgtatcac
    14281 ccgccagata tcctacgttt tgtagctgaa gcaaaggact attttcctgc atcgctgttc
    14341 cctttttctg atttttacta aaaacagttt atccttcgca ggaataaggg ggaactctct
    14401 ttcagtaatc aggtataatt tgtttaatca agcagcaatt ttgctgatta tggacggtac
    14461 aacaggaggt ttttccgatg cttatcttta tcccgattct catttttgtc gcgctggtca
    14521 ttgtcggcgc gggcgtcaaa atcgtgccgc agggctatca atggacggta gaacgctttg
    14581 gtcgctatac caaaacgtta cagccaggtc taagcctggt ggtgccgttt atggatcgca
    14641 ttggtcgcaa gatcaatatg atggagcaag tgctcgacat cccttcccag gaagttatct
    14701 cgaaagataa cgccaacgtt accatcgacg ctgtgtgctt tattcaggtg attgacgcgc
    14761 cgcgcgcggc ttatgaagtc agcaatctgg agctggcgat catcaacctg accatgacta
    14821 acatccgtac cgtgctgggt tcaatggaac ttgatgaaat gctctctcag cgcgacagca
    14881 ttaactcgcg tctgctgcat attgtcgatg aggccaccaa cccgtggggg attaaagtca
    14941 cccgtatcga aattcgtgat gtgcgcccgc ccgcagagct tatctcttca atgaacgcgc
    15001 agatgaaagc ggaacgtacc aaacgcgctt acatccttga agcggaaggg atccgtcagg
    15061 cggaaatcct caaagccgaa ggtgaaaaac agtcacaaat cctgaaagcg gaaggcgaac
    15121 gtcagtcggc gtttttacag gctgaagcgc gtgaacgttc cgctgaagca gaagcccgcg
    15181 ccaccaaaat ggtgtctgaa gccatcgcct ccggtgatat tcaggcggtg aactacttcg
    15241 tcgcacagaa atacaccgaa gcgttacagc agatcggttc gtccagtaac agcaaagtgg
    15301 tgatgatgcc attagaggcc agcagcctga tggggtcgat tgccgggatt gctgagctgg
    15361 tgaaagacag cgccaacaaa cggactcagc catgatggag ttaatggtcg ttcatccaca
    15421 tattttctgg ctaagtctcg gcggtttgct gctggcagcc gagatgttgg gcggcaatgg
    15481 ttatctgttg tggagcggcg tggcagcggt gattaccggt ctggtggtct ggttgctgcc
    15541 gctgggttgg gagtggcaag gggtaatgtt tgccgtcctg acgctgctcg ccgcctggct
    15601 gtggtggaaa tggttgtcgc ggcgggtgcg cgaacaaaag cacagcgaca gtcatttaaa
    15661 ccagcgcggg cagcagttga ttggccgacg ttttgttctg gaatctccgc tggtcaacgg
    15721 gcgcggtcat atgcgcgtcg gtgacagttc atggcctgtc agcgccagcg aggatctcgg
    15781 cactggtacg catgttgagg tgattgcgat agaaggaata acgctgatta tccgtgcggt
    15841 catcgcctga cgcgtcttat cacacctgga aatctgtacc aaaccccatg ccggataagg
    15901 cgtttacgcc gcatccggca tatagccccc tacatagtgt taattgaatg ttcggctaag
    15961 gagtagccat gttttccccc tttcgaaaac ctgaagtacg tctgctgaaa aataccgctg
    16021 cgggcgtcat tttttcggtg aagtccggga aaggatttct gcgtcaatgt gtgatttacg
    16081 atccgcactc gcacggtctt atccattcgc tctgctggca ggatgtcccg ttaatacgtt
    16141 tctggtccga ggctggatgc cctacctgtg cggaatttgt ctacagcggt tttgcggagg
    16201 atgaacaggg agccgcccgg tttttgtctg ctctcgaaaa ctggaatcgc ccgtggtctg
    16261 gcataacgga cgcttttgcc gcgctgacac cgccgttctc atgcatggct gatggttatt
    16321 atttgctgga agatcgtgaa ctttacccga cggatggcaa cgggcatttc ttttgggcgg
    16381 caacggacca tagttccagt aatccggcaa ccgttgccgt ctgggatcct gaatattgct
    16441 ctttcagtga caccgcaccc tgctttcttc tacccggcca gccgccatca cattttaatc
    16501 ctgagcgtgc gacgttttat cgcgataaac cggacgcccg cgctctggcc tggtatctcc
    16561 ctgattccta tctctgcgtt ctgctcgacg gacaccataa ggctactgcc gccgcgctgg
    16621 aagggcgacc gctcaaaaca ctggtgctca gcacggcaac ccattttaat gatgagcaac
    16681 aaaccctggt atttcctggc ggggaatgtc tgcacaaaac agagctgcaa taccacatac
    16741 caaaactcac cgcgtgcaaa acattaccgc ccagcgcatg ggagagtttc ggccctgata
    16801 agcacatcag cccatccgaa acctggtcag aagagttaaa gcagagcgtc agtcggtacc
    16861 cctcgctgga tcaagcctgg caaattgttg aggccgggaa tttgagcgaa actcgtatta
    16921 agagcatgat ccaacaaggt ttgggggagg atgaaaaggc tgatattatt ttgcaggcgc
    16981 tgttttccac acactcgccg ctttttattg actttgcccg attcgttatc agccatccgg
    17041 catacgccat ttatcgcccc ctcaccttcc ggttgatggc gcaaaaccga acgccgcaag
    17101 cagatgcttt ttttctggat tttgccatca atgatgacgg ggaacgtccg gagctgacga
    17161 aaataatgga cgactatttt cgcaagcctt aatcaccccg cccgatgatg acagcagccg
    17221 gagagatttt cgataatcgg gcagtcggcg ctgtcatcac cggggcaggc attcgccagc
    17281 gccagcagct ggttacgcat ggattgcagt tcttcgatat gctgttcgat ctccgctact
    17341 ttctccagcg tacgacgttt gacgtcggcg ctgtgacgct gcggatcgtt aaacagattc
    17401 accagttcgc cgctctcttc caggttaaag cccacctggc gtgcctggcg cagtaaggtc
    17461 agttcgttga gatgctgctg cgtgtaggtg cgataaccgt tttcgctgcg catcggcggc
    17521 gtcaccagcc ccttctcttc atagaagcga atggctttgc tggtcaggcc ggtaattttt
    17581 gctacatcgc taatgttcat cgttcgcgca acgcctcctg tgctttgtta aagggtttca
    17641 acaaatattc aagcacgttt ttttgccagt gcgaatatcg accgccacca caataataca
    17701 ggaagtactg cgcacagaga gatgttatac ggttttattg cgcttcatga ccattgccac
    17761 aataaggctg agtaagattg caccaataat ccatatcaac gcacggctgc cctgggacca
    17821 caggctgtag ataaagccaa cgatcaccaa caacatcacc aacgtaccaa cgataagaat
    17881 cggcaacgag gcgtgaatat catgacgtag gcggatcgcc accacaaaca ccgccagata
    17941 gcagatcaaa aaggtggcgc tggcaacgct ggcgagtgag cctaaattca gcgccgccgt
    18001 catcagcata atcagcacca cgacaatgat gttgccccag gtactctgcc gccacaggga
    18061 tttattcatt agcttcggca gttcgcgttc gctgcccatg ttgtccatga tgttaaacac
    18121 ggcgaacagg ttcgcgttaa tggctgaagc cgtcgccagt aaagcgccga tgacgacgat
    18181 cacataaccc acatgcccga gcagcggaga agcagcctgc gctacggcgg tatcagcata
    18241 tttttctaac tctaatgccg acacatcgct aagcaaaacc agcgccagcg agatataaag
    18301 caacgtggta acgccaatcg ccaccagaaa cgcccgtggc ataatgacct gcggatcttt
    18361 cactttatcc gccgcgttcg ccatcatgcc aaagcccgca taggcaagga aggttatccc
    18421 gatacaggag aagaacgcac cggagctggg gggcgcagag acggaaatat gcgccggttg
    18481 cagcgaccag acacctgcaa tgatcagcaa taacaggatc atcattttaa tgccgacgag
    18541 gatcacttcc agccgcccta ccgcatggtt gcttaaggag ttgaaaagcg tcatcaccgc
    18601 aatgatcccc aacgcgtaga gcaatataag gtgctcctcc tggctgcctt catgcaaaaa
    18661 ctgcacggca taagcgccaa aagcacgggc gaccatggcg atgcttaccg ccagcgtcaa
    18721 caggtacagt aacgagagcg ccagcgaaaa gacgccgttg cctaatccgc gacgaaagaa
    18781 gtcgataata ccgccattgc tgggatagct cgcccccagg cgcgcatagg cataaccgga
    18841 aaacatcgcc acaataccgc caaatgcaaa ggcgacccag gtcgaggctt ccattag
//
LOCUS       DAZFFE010000093        18814 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFFE010000093 DAZFFE010000000
VERSION     DAZFFE010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.093"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            219..1337
                     /gene="gcvT"
                     /locus_tag="ACHJ69_003544"
     CDS             219..1337
                     /gene="gcvT"
                     /locus_tag="ACHJ69_003544"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO5016451.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEAQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVARFTFLEID"
     gene            1337..1726
                     /gene="gcvH"
                     /locus_tag="ACHJ69_003545"
     CDS             1337..1726
                     /gene="gcvH"
                     /locus_tag="ACHJ69_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO5016452.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1844..4717
                     /gene="gcvP"
                     /locus_tag="ACHJ69_003546"
     CDS             1844..4717
                     /gene="gcvP"
                     /locus_tag="ACHJ69_003546"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO5016453.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDIASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQAAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(4883..6316)
                     /gene="bglA"
                     /locus_tag="ACHJ69_003547"
     CDS             complement(4883..6316)
                     /gene="bglA"
                     /locus_tag="ACHJ69_003547"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO5016454.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVVPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMIDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAAHRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEDDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            6361..6672
                     /gene="yqfB"
                     /locus_tag="ACHJ69_003548"
     CDS             6361..6672
                     /gene="yqfB"
                     /locus_tag="ACHJ69_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO5016455.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPDQTQFYVIEFKC
                     L"
     gene            6836..7495
                     /gene="yqfA"
                     /locus_tag="ACHJ69_003549"
     CDS             6836..7495
                     /gene="yqfA"
                     /locus_tag="ACHJ69_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO5016456.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(7572..8552)
                     /gene="ygfZ"
                     /locus_tag="ACHJ69_003550"
     CDS             complement(7572..8552)
                     /gene="ygfZ"
                     /locus_tag="ACHJ69_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO5016457.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVIIAPDDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLTLNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLKGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLRIEPLPYSLEE"
     gene            8795..9061
                     /gene="sdhE"
                     /locus_tag="ACHJ69_003551"
     CDS             8795..9061
                     /gene="sdhE"
                     /locus_tag="ACHJ69_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO5016458.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            9042..9449
                     /gene="ygfX"
                     /locus_tag="ACHJ69_003552"
     CDS             9042..9449
                     /gene="ygfX"
                     /locus_tag="ACHJ69_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO5016459.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9489..10010)
                     /gene="fldB"
                     /locus_tag="ACHJ69_003553"
     CDS             complement(9489..10010)
                     /gene="fldB"
                     /locus_tag="ACHJ69_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO5016460.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDAPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            10122..11018
                     /gene="xerD"
                     /locus_tag="ACHJ69_003554"
     CDS             10122..11018
                     /gene="xerD"
                     /locus_tag="ACHJ69_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO5016461.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            11043..11753
                     /gene="dsbC"
                     /locus_tag="ACHJ69_003555"
     CDS             11043..11753
                     /gene="dsbC"
                     /locus_tag="ACHJ69_003555"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO5016462.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            11759..13492
                     /gene="recJ"
                     /locus_tag="ACHJ69_003556"
     CDS             11759..13492
                     /gene="recJ"
                     /locus_tag="ACHJ69_003556"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO5016463.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDPQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            13583..14681
                     /gene="prfB"
                     /locus_tag="ACHJ69_003557"
     CDS             join(13583..13657,13659..14681)
                     /gene="prfB"
                     /locus_tag="ACHJ69_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO5016464.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            14691..16208
                     /gene="lysS"
                     /locus_tag="ACHJ69_003558"
     CDS             14691..16208
                     /gene="lysS"
                     /locus_tag="ACHJ69_003558"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO5016465.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(16251..16799)
                     /gene="idi"
                     /locus_tag="ACHJ69_003559"
     CDS             complement(16251..16799)
                     /gene="idi"
                     /locus_tag="ACHJ69_003559"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO5016466.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEEAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLAAVLRGIDATPWAFSP
                     WMVMQATNREARKRLSAFTQLK"
     gene            16854..16925
                     /locus_tag="ACHJ69_003560"
     CDS             16854..16925
                     /locus_tag="ACHJ69_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016467.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(16922..17047)
                     /gene="yqfG"
                     /locus_tag="ACHJ69_003561"
     CDS             complement(16922..17047)
                     /gene="yqfG"
                     /locus_tag="ACHJ69_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO5016468.1"
                     /translation="MNLLMRAIFSLLLIFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(17049..18497)
                     /gene="uacT"
                     /locus_tag="ACHJ69_003562"
     CDS             complement(17049..18497)
                     /gene="uacT"
                     /locus_tag="ACHJ69_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO5016469.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     IASGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         4613 a         4704 c         5017 g         4480 t
ORIGIN      
        1 gttttttggt tttttattct gttgcgattt tggcattttt taaccataag ctaatgtgat
       61 gatcaatttt accttatggt taacagtctg tttcggtggt aagttcaggc aaaagagaac
      121 gattgcgttg gggaccggga gtggctccga tgctgggttt cgtggtgata atttcaccat
      181 gaaaaagttg tcagccccgc ttattcaatg aggacaagat ggcacaacag actcctttgt
      241 atgaacaaca cacgctttgc ggcgctcgca tggtggattt ccacggctgg atgatgccgc
      301 tgcattacgg ttcgcaaatc gacgaacatc atgcggtacg taccgatgcc ggaatgtttg
      361 atgtgtcaca tatgaccatc gtcgatcttc gcggcagccg cacccgggag tttctacgtt
      421 atctgctggc gaacgatgtg gcgaagctca ccaaaagcgg caaagccctt tactcgggga
      481 tgttgaatgc ctctggcggt gtgatagatg atctcatcgt ctactacttt actgaagatt
      541 tcttccgcct cgttgttaac tccgccaccc gcgaaaaaga cctctcctgg attacccaac
      601 acgctgaacc tttcggcatc gaaattactg ttcgtgatga cctttccatg atcgccgtac
      661 aagggccgaa tgcgcaggca aaagctgcca cactgtttaa tgacgcccag cgtcaggcgg
      721 tggaagggat gaaaccgttc tttggcgtgc aggcgggcga tctgtttatt gccaccaccg
      781 gttacaccgg tgaagcgggc tacgaaattg cgctgcccaa tgaaaaagcg gccgatttct
      841 ggcgcgcgct ggtggaagct ggcgttaagc catgtggcct gggcgcgcgt gacacgctgc
      901 gtctggaagc gggcatgaat ttgtatagcc aggagatgga cgaaactatt tctcctttag
      961 ccgccaatat gggctggact atcgcctggg aaccggcaga tcgtgacttt atcggtcgtg
     1021 aagctctgga agcgcagcgt gaacatggca cagaaaaact ggttggtctg gtgatgaccg
     1081 aaaaaggcgt gctgcgtaat gaactgccgg tacgttttac tgatgcgcag ggcaaccagc
     1141 atgaaggcat tatcaccagc ggtactttct ccccaacgct gggttatagc attgcgctgg
     1201 cgcgcgtgcc ggaaggtatt ggtgaaacgg cgattgtgca aattcgcaac cgtgaaatgc
     1261 cggttaaagt gacgaaacct gtttttgtgc gtaacggcaa agccgtcgcg agatttactt
     1321 ttttggagat tgattgatga gcaacgtacc agcagaactg aaatacagca aagaacacga
     1381 atggttgcgt aaagaagccg acggcactta caccgttggc atcaccgaac acgctcagga
     1441 gctgttaggc gatatggtgt ttgttgacct accggaagtg ggcgcaacgg ttagcgcggg
     1501 cgatgactgc gcggttgccg aatcggtaaa agcggcgtca gatatttatg cgccagtaag
     1561 cggtgaaatc gtggcggtaa acgatgcact gagcgattct ccggaactgg tgaacagcga
     1621 accgtatgca ggcggctgga ttttcaaaat caaagccagc gatgaaagcg aactggaatc
     1681 actgctggat gcaaccgcat acgaagcatt gttagaagac gagtaacggc tttattcctc
     1741 ttctgcggga gaggatcagg gtgaggaaat ttacgcctca ccctcactct cttcgcaagg
     1801 agagaggttc acaattcact gcacgtttca ggaaccatcg ctcatgacac agacgttaag
     1861 ccagcttgaa aacagcggcg cttttattga acgccatatc ggaccggacg ccgcgcaaca
     1921 gcaagaaatg ctgaatgccg ttggtgcaca atcgttaaac gcgctgaccg gccagattgt
     1981 gccgaaagat attcagcttg cgacaccacc gcaggttggc gcaccggcga ccgaatacgc
     2041 cgcactggca gaactcaagg ctattgccag tcgcaataaa cgcttcacgt cttacatcgg
     2101 catgggttac accgccgtgc agctaccgcc ggttatcctg cgtaacatgc tggaaaatcc
     2161 gggctggtat accgcgtata ctccgtatca acctgaagtc tcccagggcc gccttgaagc
     2221 actactcaac ttccagcagg taacgctgga tttgaccggg ctggatatag cttcggcttc
     2281 tcttctggat gaggccactg ctgccgccga agcaatggcg atggcgaaac gcgtcagtaa
     2341 actgaaaaat gccaaccgct tctttgttgc ttctgacgtt catccgcaaa cgctggatgt
     2401 ggtccgtact cgtgccgaaa cctttggttt tgaagtgatt gtcgatgacg cgcaaaaagt
     2461 gctcgaccat caggacgtct tcggcgtgct gttacagcag gtcggcacta ctggtgaaat
     2521 tcacgactac accgcgctta tcagcgaact gaaatcacgc aaaatcgtgg tcagcgttgc
     2581 cgccgatatt atggcgctgg tgctgttaac tgcgccgggt aaacagggcg cggatattgt
     2641 ttttggttcg gcgcaacgct ttggcgtacc gatgggctat ggtggcccac acgcggcatt
     2701 ctttgcggcg aaagacgaat acaaacgctc aatgccgggc cgtattatcg gtgtatcgaa
     2761 agatgcagct ggcaacaccg ctctgcgcat ggcgatgcag actcgcgagc aacatattcg
     2821 tcgtgagaaa gcgaactcca acatttgtac ttcccaggta ctgctggcaa acattgccag
     2881 cctgtatgcc gtttatcacg gtccggttgg cctgaaacgt atcgctaatc gcatccaccg
     2941 tctgaccgat atcctggcgg cgggcctgca acaaaaaggt ctgaagctgc gccatgcgca
     3001 ctatttcgat actttgtgtg tggaagtggc cgacaaagcg ggcgtgctgg cgcgtgccga
     3061 agcggctgaa atcaacctgc gtagcgatat tctgaacgcg gttgggatca cccttgatga
     3121 aacaaccacg cgcgaaaacg tgatgcagct tttcagcgtg ctgttgggcg ataaccacgg
     3181 gctggacatc gacacactgg acaaagacgt ggctcacgac agtcgctcta ttcaggctgc
     3241 gatgctgcgc gacgacgaaa tcctcaccca tccggtgttt aatcgctacc acagcgaaac
     3301 cgaaatgatg cgctatatgc actcgctgga gcgtaaagat ctggcgctga atcaggcgat
     3361 gatcccgctg ggttcctgca ccatgaaact gaacgccgcc gccgagatga tcccgatcac
     3421 ctggccggaa tttgccgaac tgcacccgtt ttgcccgcca gaacaggctg aaggttatca
     3481 gcagatgatc gcgcagctgg ctgactggct ggtgaaactg accggttacg acgccgtttg
     3541 tatgcagccg aactctggcg cacagggcga atacgcgggc ctgctggcga ttcgtcatta
     3601 tcatgaaagc cgcaacgaag ggcatcgcga tatctgcctg atcccggctt ctgcgcacgg
     3661 cactaacccg gcttccgcgc atatggcagg aatgcaggtg gtggttgtgg cgtgtgataa
     3721 aaacggcaac atcgatctga ctgatctgcg cgcgaaagcg gaacaggcgg gcgataacct
     3781 ctcctgtatc atggtgacct atccttctac ccacggcgtg tatgaagaaa cgatccgtga
     3841 agtgtgtgaa gtcgtgcatc agttcggcgg tcaggtttac cttgatggcg cgaacatgaa
     3901 cgctcaggtt ggcatcacct cgccgggctt tattggtgcg gacgtttccc accttaacct
     3961 gcataaaact ttctgcattc cgcatggcgg tggtggtccg ggtatgggac cgatcggtgt
     4021 gaaagcgcat ctggctccgt ttgtaccggg tcatagcgtg gtgcaaatcg aaggcatgtt
     4081 aacccgtcag ggcgcggttt ctgcggcacc gttcggtagt gcctctatcc tgccaatcag
     4141 ctggatgtac atccgcatga tgggcgcaga agggctgaaa aaagcaagcc aggtggcaat
     4201 tcttaacgcc aactatattg ccagccgtct gcaggatgcc ttcccggtgc tgtataccgg
     4261 tcgcgacggt cgcgtggcgc acgaatgtat tctcgatatt cgcccgctga aagaagaaac
     4321 cggcatcagc gagctggata ttgccaagcg cctgatcgac tacggtttcc acgcgccgac
     4381 catgtcgttc ccggtggcgg gtacgctgat ggttgaaccg actgaatctg aaagcaaagt
     4441 ggaactggat cgctttatcg atgcgatgct ggctatccgc gcagaaattg atcaggtgaa
     4501 agccggtgtc tggccgctgg aagataaccc gctggtgaat gcgccgcaca ttcaaagcga
     4561 actggtcgcc gagtgggcgc atccgtacag ccgtgaagtt gcagtattcc cggcaggtgt
     4621 ggcagacaaa tactggccga ccgtgaaacg tctggatgat gtttacggcg accgtaacct
     4681 gttctgctcc tgcgtaccga ttagcgaata ccagtaattt actgattcga ctatcttata
     4741 aaggcgcttc ggcgcctttt tcgcttgtta ataaagtgcg ctttgtgtgt ttcggctgag
     4801 gcgtaatata aaatattgcg agctcaatat attaaaagag tgccaggcct gatgagcgga
     4861 gcacatcagg cacatcggct aattacagat tctcgccgtt gctggcaatc acctctttgt
     4921 accagttaaa gctcttctta cgtgaacgcg acatatcgcc agtaccgtcg tcatgtttat
     4981 tcacatagat aaagccgtag cgtttgctgt actgcccggt ggtgaacgac acacagtcga
     5041 tgcagcccca cggcgtgtag cccatcaggt ccacgccatc gtaagtcacc gcttttttca
     5101 tctcttcaat atgggcacgc aggtaatcaa tgcggtaatc gtcattgatg ctgccatcat
     5161 cttccacttt gtcgtaagcg ccaaaaccgt tttcgacaat aaacagcggc ttctggtaac
     5221 gctcatacag ctcgcaaagc gcgtaacgca gaccaaccgg atcaatctgc cagccccagt
     5281 cagacgcttt aacatacggg ttcggtacgc tgccttcaaa accagagatc gcatcgccgg
     5341 tgccgccttc ggccttcacc gcgttggtca tgtaatagct gaagccgaga taatcgcagg
     5401 tgccttcgcg cagtacatcc agatcgccgt cttccatttt gatgttaaat ccgcggcgtt
     5461 cccactcgtt caacacatag gacgggtaat agccacgcaa ctgcacatcg gtaaagacgt
     5521 aacgttcgcg catcgactcc tgggcaaaca tcacatcgtc cgggttacag gagtaaggat
     5581 agagcggcac catcgccagc atacaaccga ctttcatctc cgggttgatg cgatgcgcgg
     5641 ctttcaccgc cagggcgctg gcgacaaact ggtgatgcag cacctgatac atcgtctctt
     5701 ccgggttttc atgctcggta tacaccacgc cggagcagca gtaaccgaac agcggtgcac
     5761 gccagttacg ctggttgtta atttcgttga aggtcatcca gtatttgact ttatgcttat
     5821 agcgttcaaa taccacttcc gcgaaacgga caaagaaatc aaccacttta cggttggtcc
     5881 agctaccata ttgctgcacc agatgcagag gcatttcaaa gtgggagagg gtgatcaccg
     5941 gttcgatgtt gtatttcagc agttcatcga tcatatcatc gtagaacttc agcccttctt
     6001 cgtttggctg agcttcatcg ccttttggaa aaatgcgcgt ccaggcgatg gaagtacgaa
     6061 aacatttgaa gcccatttcg gcaaatagct tgatgtcctc cttgtagtga ccataaaaat
     6121 caacggcttc atggtttgga tagtatttgc caggcacaac ttctttggtg atttcgcgcg
     6181 gcacgccgtg tgcgccaccg gtcaaaacgt cacaaatgct cggccctttg ccgcctttgt
     6241 tccagccgcc ttcgacctga tgagcggcaa ctgcgccgcc ccataagaaa tctttcggta
     6301 aggtgagttt tttcactatc ataggctcca gatgagtcaa ttcttacagg aaatattacc
     6361 atgcagccaa acgacatcac tttttttcaa cgtttccagg atgacattct ggctgggcgt
     6421 aaaaccatca ccatccgcga cgagtctgaa tcgcacttca aaacgggtga tgtgcttcgt
     6481 gtcggacgtt ttgaagatga cggttatttt tgcacgattg aagtcaccgc aacctcaacc
     6541 gtaacgctgg atacgctgac ggaaaaacat gcagagcagg aaaatatgac cctgactgaa
     6601 ctgaaaaagg tcattgccga catctatccc gaccagacac agttttatgt gattgaattt
     6661 aaatgtcttt aaattgaact cacgcctgtt agttgaaatt ttgcatttaa tttcatgatt
     6721 tttcggagat taatctcaaa tcagaattta ttttagctaa caggtgttca ctggaactat
     6781 tctcagttac gctggagagg tatacatgtg cgtgtacgtt tttccggagt aagttatggt
     6841 tcagaagccc ctcattaagc agggatattc gctggcagag gaaattgcca acagcgtcag
     6901 tcacggcatt gggctggtgt ttggtatcgt tgggctggtg ttgctactgg ttcaggcggt
     6961 ggatctcaat gccagcgcca cggcgataac cagttacagc ctctatggcg gcagtatgat
     7021 cctgctgttc ctcgcttcga cgctctatca cgccattccc catcaacggg caaaaatgtg
     7081 gctgaagaaa tttgaccact gtgccattta tctgttgatt gccggaacct acacgccgtt
     7141 tttgctggtg gggctggatt ctccgttagc gcgcgggttg atgattgtta tctggagcct
     7201 ggcattgctg ggtattctgt ttaaactgac catcgcgcac cgattcaaaa ttttatctct
     7261 ggtgacctat ctagcgatgg gctggctgtc gctggtggta atttatgaaa tggcagttaa
     7321 gctcgcggcg ggcagcgtta ccttactggc ggtaggcggt gtggtttatt cgctcggggt
     7381 gattttctac gtctgcaagc gcattccata caatcatgcc atctggcacg gctttgtgct
     7441 cggcggtagt gtgtgccact ttctggcgat ctatttgtat attgggcagg cgtaattggc
     7501 gtgcctttgt aggcctgaaa agacacacca gtgtcgcatc aggcgataaa tgcctgatgc
     7561 gatacagaga tttactcttc cagcgaatat ggcagtggct cgatacgcaa tgtattcgca
     7621 tcgtcacgca cgcggaagat gctgtccggt tccatatcgt tattcatgac aacctgcacc
     7681 acaacctgac catcttccag tttgaccgca gccagcaccg taccggtacg acgccagttc
     7741 tcgcccattt tcagctctaa gtcttcacca gcttccggca gtcggctggc gctacccttc
     7801 agcaaccaga gcgcgcgttt gttggcacca cggaattttg ctcgcgccac catctcttgt
     7861 ccggtatagc agcctttctt aaagctgata ccgcccagcg cctggaggtt ggtcgcctgg
     7921 gggataaact gcccgctgtt ggcggcatca atcaccggaa aacccgcttc aatgtttaat
     7981 gtcagccact gttggctatt gttcagttcc gcttcaccgc gcagtttatc ggtcagcata
     8041 ttggcagtag cttcatcggt tacgatcagg aaacgttctg ccgggtgttc aaaccatagc
     8101 aacgtggtcg cgccttcttt gactacctgt ttttctctcg aaggtagttc actaaagaga
     8161 tttgccaacg cggcgcgcgc ctgaaaaccg gcaacaccaa gcagcacacg ctcgtcgtct
     8221 ggcgcgatga tcactttaga gaacaccgca tattttttca gttcagtcag ctgcggttca
     8281 cgcacgctgc gccgttcaat ccatgcaaag ccatcgccgt cgcggaacag acgtaaattg
     8341 ctccacattt tacctttggc gtcgcaatgg gcggcgagca ggtgctgatc ttcagtcatc
     8401 tggctgacat ctgctgtcac ctggccttgc atatattttt cgctgtccgc gccagtaata
     8461 gtggcaagcg cccagtcatc aagcgtcatc agcgtcagtg gcaaacgggc agaagccgta
     8521 ggctgacggg gaggaaaagg tgtaaaagcc ataatagagt cctgagttgc ttaacggcgt
     8581 attaataact aatggtaaaa gagctatgag gcaatgcaag tgctttcact acgcgatctg
     8641 tgccttctgc gaggcgactt ccaaaaaaat gaagcccttg aattgttgtg aattaatttc
     8701 gcgatcgcga ttgtgtttac tgatattccc gtaaaagaag tcgaaaaaca cgttacaata
     8761 gccgggtact ggattttcgt gagacaggaa gaagatggac attaacaaca aagcccgcat
     8821 tcattgggca tgccgccgtg gtatgcgcga actcgatatt tcaatcatgc cgtttttcga
     8881 acatgagtac gacagcttaa gcgatgacga aaaacgcatc tttattcgtc tgctggaatg
     8941 tgacgatccg gacctgttta actggctgat gaatcacggt aaaccggccg atgcagaact
     9001 ggaaatgatg gtccgactca ttcagacacg gaaccgggaa cgtggtcctg tggcaatctg
     9061 atttgcgcgt ctcctggcgc gcacagtggc tttccttgct gattcatggg ctggttgccg
     9121 ctgttatttt actcatgccc tggccgctca gttacacccc gttatggatg gtgttacttt
     9181 cgctggtggt gtttgattgc gttcgcagcc agcggcgcat taatgctcgc cagggggaaa
     9241 ttcgcttgtt gatggacggg cgtttgcgtt ggcaagggca ggagtggagc atcgtcaaag
     9301 cgccgtggat gattaagagc ggcatgatgc tgcgtttacg ttctgacagc ggaaaacggc
     9361 aacatttatg gctggcagct gacagcatgg acgaagccga atggcgggat ttacggcgga
     9421 tattgttaca acaagagacg caaagataaa gatgacggca aagcatatgt gctaagccgt
     9481 cagtgacatc aggcgtaatg ctctgccatt tcgttgagga tttgctcgca ccagctctga
     9541 atacgctcgt cgctaaggtc atactggtta gtttcatcca gcgccagacc cacgaacagt
     9601 tgcccgtcag caatcaccgg tttcgggctg gtaaattcat atccttccgt tggccagtag
     9661 ccgacgaact tcacgccttt ggtcgagagt ttgtcatgca gcataccgag cgcatcgagg
     9721 aaccactcgc cgtatcccag ttgatcgcca agcccataca gcgcaacaat tttaccttcg
     9781 aggttcaggt cgtcgagctg atcccagacg gcttcccagt cttcctggat ttcgccaaaa
     9841 tcccaggtcg ggatacccag aatgagcaca tcgtactgct ccattaattt cggggcgtcg
     9901 tccttgaggt tatgtaaggt caccagttct gggccgataa tatcgcggat tttttctgcc
     9961 gccatttcgg tgtaacaggt gctggaaccg taaaaaagac ccatattcat aacgtaagct
    10021 tctcgattct gctgtggctt ttgcggctag tgtatcagaa tcgctttagt cacggcacaa
    10081 taaggcataa tggatcaaat gagtgaccat aaggggcgca agtgaaacag gatctggcac
    10141 gcatcgagca gtttcttgat gctctgtggc tggaaaaaaa tctggctgaa aatacgttga
    10201 acgcttaccg tcgcgatctg tcaatgatgg tggagtggtt gcatcaccgc gggttgacgc
    10261 tggcgacggc gcaaagtgac gatttgcagg cattactggc agaacggctg gagggcgggt
    10321 ataaagccac cagctcagcg cgtttgctga gtgcagtgcg ccgattgttc cagtatcttt
    10381 atcgcgaaaa gtttcgtgaa gatgatccca gtgcgcatct cgcttcaccg aaattgcccc
    10441 agcgtttgcc aaaagattta agcgaagcgc aggtcgaacg tttattacag gcaccattaa
    10501 ttgatcagcc actggagcta cgcgataaag ccatgcttga agtgttgtat gctaccggac
    10561 tgcgtgtctc tgaactggtc gggctgacaa tgagtgatat cagcctgcgt cagggcgtgg
    10621 tgcgggtcat tggtaaaggc aacaaagagc gcctggtgcc gttaggtgaa gaggcggttt
    10681 actggctgga aacctatctg gaacatgggc gtccgtggct gttgaatggt gtgtcaattg
    10741 acgtgttgtt tcccagccag cgtgcgcagc agatgacgcg acagaccttc tggcaccgca
    10801 ttaaacatta tgccgtgctg gcgggtatcg acagcgaaaa gctgtcaccg catgtgttgc
    10861 gtcacgcttt tgccactcac ttattaaatc atggtgcgga tttgcgcgtg gtgcagatgc
    10921 tactgggcca cagcgatctc tccaccacgc aaatttatac gcatgtcgct accgagcgtc
    10981 tgcggcaact tcatcaacag catcacccgc gggcgtgatg tctgaaaaga acaggaaaat
    11041 ttatgaagaa aggttttatg ttatttacct tgttggcagc gttttcaggc tttgttcagg
    11101 ctgatgacgc ggcaattcaa caaacgttag ccaaaatggg catcaaaagc agcgatattc
    11161 agcccgcgcc cgtagctggc atgaagacag ttctgactaa cagcggtgtg ttgtacatca
    11221 ccgatgatgg taaacatatc attcaggggc caatgtatga cgttagtggc acggctccgg
    11281 tcaatgtcac caataagatg ctgttaaagc agttgaatgc gcttgaaaaa gagatgatcg
    11341 tttataaagc gccgcaggaa aaacacgtca tcaccgtgtt tactgatatt acctgtggtt
    11401 actgccacaa actgcatgaa caaatggcag actataacgc gctggggatc accgtgcgtt
    11461 atcttgcttt cccgcgccag gggctggaca gcgatgcaga gaaagaaatg aaagctatct
    11521 ggtgtgcgaa agataaaaac aaagcgtttg atgatgtgat ggcaggtaaa agcgtcgcac
    11581 cagccagctg cgacgtggat attgccgacc attacgcact tggcgtccag cttggcgtta
    11641 gcggtactcc ggcagttgtg ctgagcaatg gcacacttgt tccaggttac cagccgccga
    11701 aagacatgaa agaattcctc gacgaacacc aaaaaatgac cagcggtaaa taattcgcgt
    11761 gaaacaacag atacaacttc gtcgccgtga agtcgatgaa acggccgact tgcccgctga
    11821 attgcctccc ttgctgcgcc gtttatatgc cagccggggt gtgcgtagcg cgcaggaact
    11881 ggaacgcagt gttaaaggca tgctgccctg gcagcaactg agcggcgtcg aaaaggccgt
    11941 tgagatcctt tacaacgctt ttcgcgaagg cacgcggatt attgtggtcg gtgatttcga
    12001 cgccgacggt gccaccagca cggctctaag cgtgctggcg atgcgctcgc ttggttgcag
    12061 caatatcgac tacctggttc caaaccgttt cgaagacggt tacggcttaa gcccggaagt
    12121 agtcgatcag gctcatgccc gtggcgcgca gttaattgtc acggtggata acggtatttc
    12181 ctcccatgcg ggggttgaac acgctcgttc gttgggcatt ccggttattg ttaccgatca
    12241 ccatttgcca ggcgacacat tacccgcagc ggaagcaatc attaacccta acttgcgcga
    12301 ctgtaatttc ccgtcgaaat cactggcagg cgtgggtgtt gcgttttatc tgatgctggc
    12361 gctgcgcacc tttttgcgcg atcagggctg gtttgatgag cgcggcatcg caattcctaa
    12421 tctggcagaa ctgctggatc tggtagcgtt ggggacagtg gcggacgtcg tgccgctgga
    12481 cgctaataat cgcattctga cctggcaggg gatgagtcgc atccgtgccg gaaagtgccg
    12541 tccggggatt aaagcgctgc tggaagtggc caatcgcgat ccacaaaaac tcgccgccag
    12601 cgatttaggt tttgcgctgg ggccacgtct caatgctgcc gggcgactgg acgatatgtc
    12661 cgttggcgtg gcgctgttgt tgtgtgacaa cattggcgaa gcgcgtgtgc tggcgaatga
    12721 actcgatgca ctaaaccaaa cacgtaaaga gatcgaacag ggaatgcagg ttgaagcgct
    12781 cactctgtgc gagaagctgg aacgtagccg cgacacgctg ccaggtgggc tggcaatgta
    12841 tcaccccgaa tggcatcagg gcgttgttgg cattctggca tcgcgtatca aagagcgttt
    12901 tcaccgtccg gttatcgcct ttgcgccagc tggtgatggt acgctgaaag gttccggtcg
    12961 ctccattcag gggctgcata tgcgtgatgc gctggagcga ttagacacac tctaccccgg
    13021 catgatgctc aagtttggcg gtcatgcgat ggcggcgggt ttgtcgctgg aagaggataa
    13081 attcgaactc tttcaacaac ggtttggcga actggttacg gagtggttgg acccttcgct
    13141 attgcaaggc gaagtggtgt cggacggtcc gttaagtccg gccgaaatga ccatggaagt
    13201 ggcgcagctg ttgcgcgatg ctggcccgtg ggggcagatg ttcccggagc cgctgtttga
    13261 cggtcatttt cgtctgctgc aacagcggct ggtgggcgaa cgtcatttga aagtcatggt
    13321 cgaaccggtc ggcggcggtc cactgctgga cgggattgct tttaatgtcg ataccgccct
    13381 ctggccggat aacggtgtgc gcgaagtgca actggcttac aagctcgata tcaacgagtt
    13441 tcgcggcaac cgcagcctgc aaattatcat cgacaatatc tggccaattt agcgtcatct
    13501 tctctataaa aaagagcgtg gattgggtac aatcccgctc ttatcaccgc attttgacta
    13561 gctcaataaa agaaatcaga ccatgtttga aattaatccg gtaaataatc gcattcagga
    13621 cctcacggaa cgctccgacg ttcttagggg gtatctttga ctatgacgcc aagaaagagc
    13681 gtctggaaga agtaaacgcc gagctggaac agccggatgt ctggaacgaa cccgaacgcg
    13741 cacaggcgct gggtaaagag cgttcctccc tcgaagccgt tgtcgacacc ctcgaccaaa
    13801 tgaaacaggg gctggaagat gtttctggtc tgctggaact ggctgtagaa gctgacgacg
    13861 aagaaacctt taacgaagcc gttgctgaac tcgacgccct ggaagaaaaa ctggcgcagc
    13921 ttgagttccg ccgtatgttc tctggcgaat atgacagcgc cgactgctac ctcgatattc
    13981 aggcggggtc tggcggtacg gaagcacagg actgggcgag catgcttgag cgtatgtatc
    14041 tgcgctgggc tgaatcgcgt ggtttcaaaa ctgaaatcat cgaagagtcg gaaggtgaag
    14101 tggcgggtat taaatccgtg acgatcaaaa tctccggcga ttacgcttat ggctggctgc
    14161 gtacagaaac cggcgttcac cgcctggtgc gtaagagccc gtttgactcc ggcggtcgtc
    14221 gccacacgtc gtttagttca gcgtttgttt acccggaagt tgatgatgat atcgatatcg
    14281 aaatcaatcc ggcggatctg cgcatcgacg tttatcgcgc atctggcgcg ggcggtcagc
    14341 acgttaaccg taccgaatct gcggtgcgta ttactcacat cccaaccggg atcgtgaccc
    14401 aatgccagaa cgaccgttcc cagcacaaga acaaagacca ggccatgaag cagatgaaag
    14461 cgaagcttta tgaactggag atgcagaaga aaaatgccga gaaacaggcg atggaagata
    14521 ataaatccga catcggctgg ggcagccaga ttcgttctta tgtccttgat gactcccgca
    14581 ttaaagatct gcgtaccggg gtagaaaccc gcaacacgca ggccgtgctg gacggcagcc
    14641 tggatcaatt tatcgaagca agtttgaaag cagggttatg aggaaccaac atgtctgaac
    14701 aacacgcaca gggcgctgac gcggtagtcg atcttaacaa tgaactgaaa acgcgtcgtg
    14761 agaagctggc gaacctgcgt gagcagggga ttgccttccc gaacgatttc cgtcgcgatc
    14821 atacctctga ccaattgcac gcagaattcg acggtaaaga gaacgaagaa ctggaagcgc
    14881 tgaacatcga agtcgccgtt gctggccgca tgatgacccg tcgtattatg ggtaaagcgt
    14941 ctttcgttac tctgcaggac gttggcggtc gcattcagtt gtacgttgcc cgtgacgatc
    15001 tgccggaagg cgtttacaac gagcagttca aaaaatggga cctcggcgat atcctcggcg
    15061 cgaaaggtaa actgttcaaa accaaaaccg gcgaactgtc tatccactgt accgagctgc
    15121 gtctgctgac caaagcactg cgtccgctgc cggataaatt ccacggcttg caggatcagg
    15181 aagcgcgcta tcgtcagcgt tatctggatc tcatctctaa cgatgaatcc cgcaacacct
    15241 ttaaagtgcg ctcgcagatc ctctctggta ttcgccagtt catggtgaat cgcggcttta
    15301 tggaagttga aacgccgatg atgcaggtga tccctggcgg tgccgctgcg cgtccgttta
    15361 tcactcacca taacgcgctg gatctcgaca tgtacctgcg tatcgcgccg gaactgtacc
    15421 tcaagcgtct ggtggtcggt ggcttcgagc gtgtattcga aatcaaccgt aacttccgta
    15481 acgaaggtat ttccgtacgt cataacccag agttcaccat gatggaactc tatatggctt
    15541 acgcagatta caaagatctg atcgagctga ccgaatcgct gttccgtact ctggcacagg
    15601 atattctcgg taagacggaa gtgacctacg gcgacgtaac gctggacttc ggtaaaccgt
    15661 tcgaaaaact gaccatgcgt gaagcgatca agaaatatcg cccggaaact gacatggcgg
    15721 atctggacaa cttcgactct gcgaaagcga ttgctgaatc tatcggcatc cacgttgaga
    15781 agagttgggg tctgggccgt atcgttaccg agatcttcga agaagtggca gaagctcatc
    15841 tgatccagcc gaccttcatt actgaatatc cggcagaagt ttctcctctg gcgcgtcgta
    15901 acgacgttaa cccggaaatc acagaccgct ttgagttctt cattggaggg cgtgaaatcg
    15961 gtaacggctt tagcgagctg aatgacgcgg aagatcaggc gcagcgcttc ctggatcagg
    16021 ttgccgcgaa agatgcaggt gacgacgaag cgatgttcta cgacgaagat tatgtcaccg
    16081 cactggaaca tggcttaccg ccgacagcag gtctgggaat tggtatcgac cgtatggtaa
    16141 tgctgttcac caacagccat accatccgcg acgttattct gttcccggcg atgcgtccgg
    16201 taaaataagc attacgttat gctcacaacc ccggcaaccg tcggggtttt ttatttaagc
    16261 tgggtaaatg cagataatcg ttttctggct tcgcgatttg tcgcctgcat caccatccac
    16321 ggactgaacg cccacggcgt agcatcaata ccgcgtaaaa ccgctgctaa atcacaccat
    16381 tgataatcca tcacttcatc atcgttgatc tgtaacgcac tggtcgtgcg tgcggcaaat
    16441 accggacaca cttcattttc cacaatgcca ttcggatcgg tggcgcggta gcgaaagtca
    16501 ggatagatag attcaggagg cgtaatttct acgccaagct cataacggca acggcggatc
    16561 accgcctctt cgttgctttc tcccagttgt gggtgcccac aaaccgagtt agtccacacg
    16621 ccaggccatg cttttttgct aagggcgcgg cgggtaacta ataattgccc tttggcatta
    16681 aacagccaac tggaaaacgc gagatgtaag agggtgtctg ccgtgtgtgc ggcatacttt
    16741 tccagcgtac ccgtgggaac tccctgtgca ttcaataaaa tgacgtgttc cgtttgcata
    16801 atttctcaca tgtaattctg atcactcatc attatagata atagaagagt gaaatgatta
    16861 accaagtgag cgtttatcga caaccgcccg ttttgagcgg atgtcgacag gtaaaaacca
    16921 tttaaaaaag cagcatcata tatttgaagc gactttcaat ggccattgca acacaatcag
    16981 aaatgacagg aatagagaga gtaaaaatca acagcagact aaatatagcg cgcattaaaa
    17041 gattcatatt attctccatg ctcatttttc ttcagcttgc gcatcagaag ccacatacgt
    17101 actgtcctga cttttaaatc tttatcagag acggattcct tcaccaggtc agcatgatgc
    17161 tggtagccat taaagaagac gttcagcaca acggcgctaa gggttgcgag cataatgccg
    17221 ctatgcagca acggttgcag tacggccggt aacttagaaa agaaatcgtg agagagcgtt
    17281 ggcgtcatgc caacaccgag actgatcgcc acaatataga ggttgtaacg gttagtggtg
    17341 tagttacagc gcgacagaat tcgaatccct gtcgccagta ccatgccgaa catcaccagc
    17401 cccgcgccgc ccagcacaaa ttgcggaatg gaggccacca gcaccgccat tttgggcacc
    17461 atgccgaaca ggatcaaaat gataccggaa gcaatgcaca cccagcggct atgaacgcgc
    17521 gtcacgctaa ccaggccaac gttttgagaa aatgacgtgt gggggaagct gttaaacgtt
    17581 ccgcctatca ttgtccctac accatcgaca cgcagcccgc gaataatatc gtgtgaagag
    17641 agtttacgac cgactatttc acccagtgcc aggaacatcc ccatcgactc gataaacacg
    17701 atgattaaca cggcagtcat ggtcagaatg gaaacgggat cgaaaatcgg cataccaaat
    17761 gacatcggcg tgacaatcgc aaaccatgaa gcatcatgta gcccggataa attgacttca
    17821 ttcatcatcc acgaaagtaa aaagccaaat acaatcccca gtaatacggc gacgttggac
    17881 ataaatcctt tcgcatagcg agtaatgagc aagataaaaa ttaagacggc aaaggagata
    17941 cctaaataaa cgggattacc atattgcgga ttccctttac ctccggcggc ccagtcaata
    18001 cccacctgaa tgatgctaag cccgatagaa gtaataacca caccggtaac cagtggcggg
    18061 aataaaggca tcaagcgacc gataagtggc gctaataatg tggtgataaa acccgcggcg
    18121 atagtggcac cgaatatccc cagcaggccg atatccgggt tcataccaat agctatcatt
    18181 ggtgttacag cggcgaaggt caccgacata atcaccggca ggcggatccc cataaagcgg
    18241 ccgataccga tacattgcaa taatgtgacg atcccgcagc aaaagagatc cgagctgatg
    18301 agcatcgcaa tagcttcttt tgataggccg agtcggtcac caatcataag aggaacagcg
    18361 actgcacctg cgtacatgac aaggacgtgt tgcagaccga gtatgatcag ctttccaggt
    18421 gataatatgc ggtcaacctc atcagttggg cggtcttgcc cagaagatga gggaagttgg
    18481 gaatctatgg cgctcatcag tgtttctcct ttcgcccgcc agagtaaaaa gcaaccgttt
    18541 atgttcatga aactgtctaa acacatgctc gtggagtgcc agtttttgcg aggaagggga
    18601 gttagagaca ggaattgcgg aggcaatcac ataatcgtgg ttaaaccacg aaaatgagaa
    18661 gagaacctga caatggaatt atctgtaatt ttctcatatt gatatttcat taatcattct
    18721 ggcatgtcgc actctcgcat ttaatcgttt ttatctggat agcattcttt tgatcggcaa
    18781 tgttgaaata atgaaagcgg catagattga gggg
//
LOCUS       DAZFFE010000094        18466 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZFFE010000094 DAZFFE010000000
VERSION     DAZFFE010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.094"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            51..1190
                     /gene="arnB"
                     /locus_tag="ACHJ69_003563"
     CDS             51..1190
                     /gene="arnB"
                     /locus_tag="ACHJ69_003563"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO5016470.1"
                     /translation="MSGFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIEAAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     HTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALKQLAGQ"
     gene            1194..2162
                     /gene="arnC"
                     /locus_tag="ACHJ69_003564"
     CDS             1194..2162
                     /gene="arnC"
                     /locus_tag="ACHJ69_003564"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO5016471.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTAACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAEGSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2162..4144
                     /gene="arnA"
                     /locus_tag="ACHJ69_003565"
     CDS             2162..4144
                     /gene="arnA"
                     /locus_tag="ACHJ69_003565"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO5016472.1"
                     /translation="MKTVVFAYHDMGCLGIEALLSAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPRGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFMNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPPRHYFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4141..5031
                     /gene="arnD"
                     /locus_tag="ACHJ69_003566"
     CDS             4141..5031
                     /gene="arnD"
                     /locus_tag="ACHJ69_003566"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO5016473.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGTPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRIQRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGTLPLGQVV
                     RGNIAGREGWLGCQQIAGSH"
     gene            5031..6683
                     /gene="arnT"
                     /locus_tag="ACHJ69_003567"
     CDS             5031..6683
                     /gene="arnT"
                     /locus_tag="ACHJ69_003567"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO5016474.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYLPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADVIDRQERLVLIQYRPK"
     gene            6680..7015
                     /gene="arnE"
                     /locus_tag="ACHJ69_003568"
     CDS             6680..7015
                     /gene="arnE"
                     /locus_tag="ACHJ69_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO5016475.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFAAVNKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSLRHWCGLAFIIG
                     GIVILGSTV"
     gene            7015..7401
                     /gene="arnF"
                     /locus_tag="ACHJ69_003569"
     CDS             7015..7401
                     /gene="arnF"
                     /locus_tag="ACHJ69_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO5016476.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7395..7661)
                     /gene="pmrD"
                     /locus_tag="ACHJ69_003570"
     CDS             complement(7395..7661)
                     /gene="pmrD"
                     /locus_tag="ACHJ69_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO5016477.1"
                     /translation="MEWLVKKTCCNKQDNRHVIMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKAAGKTQ"
     gene            complement(7771..9126)
                     /gene="menE"
                     /locus_tag="ACHJ69_003571"
     CDS             complement(7771..9126)
                     /gene="menE"
                     /locus_tag="ACHJ69_003571"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO5016478.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSF
                     AVQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPEGENTFPALASLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     VSAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNGQRVPLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQSVDLDEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9123..10085)
                     /gene="menC"
                     /locus_tag="ACHJ69_003572"
     CDS             complement(9123..10085)
                     /gene="menC"
                     /locus_tag="ACHJ69_003572"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO5016479.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEDAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWIPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLDKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDVDALERLL"
     gene            complement(10085..10942)
                     /gene="menB"
                     /locus_tag="ACHJ69_003573"
     CDS             complement(10085..10942)
                     /gene="menB"
                     /locus_tag="ACHJ69_003573"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO5016480.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10957..11715)
                     /gene="menH"
                     /locus_tag="ACHJ69_003574"
     CDS             complement(10957..11715)
                     /gene="menH"
                     /locus_tag="ACHJ69_003574"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO5016481.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEVFADYSRLY
                     VDLPGHGGSATISVDGFDDVTGLLCKTLVSYNILNFWLVGYSLGGRVAMMAACQELAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11712..13382)
                     /gene="menD"
                     /locus_tag="ACHJ69_003575"
     CDS             complement(11712..13382)
                     /gene="menD"
                     /locus_tag="ACHJ69_003575"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO5016482.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGISWQQRLGDWWQDDKPWLREAPRRESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKNERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13471..14766)
                     /gene="menF"
                     /locus_tag="ACHJ69_003576"
     CDS             complement(13471..14766)
                     /gene="menF"
                     /locus_tag="ACHJ69_003576"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO5016483.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQVYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNIVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14845..15150)
                     /gene="elaB"
                     /locus_tag="ACHJ69_003577"
     CDS             complement(14845..15150)
                     /gene="elaB"
                     /locus_tag="ACHJ69_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO5016484.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15205..15666)
                     /locus_tag="ACHJ69_003578"
     CDS             complement(15205..15666)
                     /locus_tag="ACHJ69_003578"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5016485.1"
                     /translation="MIDWQDLHHSDLSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     EGENRHILGWHNGTLVAYARILKSDDELQPVVIGRVIVSEALRGEKIGQQLMSKALES
                     CTRRWPEKPIYLGAQAHLQNFYGQFGFIPVTDIYEEDGIPHIGMAREVIQA"
     gene            15731..16648
                     /gene="rbn"
                     /locus_tag="ACHJ69_003579"
     CDS             15731..16648
                     /gene="rbn"
                     /locus_tag="ACHJ69_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO5016486.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPHGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEIFDDGLRKVTAYPMEHPLECYGYRIEEHDKLGALN
                     AQALKAAGVPPGPLFQELKAGKTIMLDDGRQINGADYLAVPVPGKALAIFGDTGPCDA
                     ALELAKGVDVMVHEATLDMAMEAKANSRGHSSTRQAAALAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFAVFSI"
     gene            complement(16769..>18466)
                     /locus_tag="ACHJ69_003580"
     CDS             complement(16769..>18466)
                     /locus_tag="ACHJ69_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HFO5016487.1"
                     /translation="SGCGPEPEDKESQQQQPSTPSDQQVLVAQQAAIKEVEQSAAAAK
                     AAADAKALAQQEVQQYSDKQTLQGRLQEAPTFARAAKANATHIANPGTARYQQFDDNP
                     VKQVAQNPLVTFSLDVDTGSYANVRRFLNQGLLPPPDAVRVEEVVNYFPSDWDIKDKQ
                     SIPASKPIPFAMRYELAPAPWNEQRTLLKVDILAKDRKSKELPASNLVFLIDTSGSMI
                     SDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISGSHKAEINAAIDSLDA
                     EGSTNGGAGLEMAYQQAAKGFIKGGINRILLATDGDFNVGIDDPKSIESMVKKQRESG
                     VTLSTLGVGDSNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLNSEMRQTLITVAKDVK
                     AQIEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIGAGKHITLLFELTLKGQKAS
                     IDKLRYAPDNKSAKSDKTKELAWLKIRWKSPQGKESQLVEFPLAFAIKAPSEDMRFRA
                     AVAAYGQKLRGSEYLNNTSWQQIKQWAQKAKGEDPQGYRAEFIRLIGLAKDLDNSQN"
BASE COUNT         4255 a         4966 c         4682 g         4563 t
ORIGIN      
        1 ctttatagca aatataagtg gacaggtatt caatgacgga tggaaaaaaa atgtcaggat
       61 ttttgccttt ttcgcgacca gcaatgggcg tagaggaact cgctgcagtt aaagaggttc
      121 tcgaatccgg ttggatcaca accggtccga agaatcaggc gcttgaacaa gctttttgcc
      181 agttgacggg aaatcagcat gccatcgcgg tcagttcagc caccgccgga atgcatatca
      241 cgctaatggc gttggaaatt ggcaagggcg atgaagtgat tacgccttcc ctgacctggg
      301 tttcaaccct caatatgatt tccttgctgg gtgcaacgcc ggtaatggtg gatgtcgacc
      361 gcgatacgct gatggtcacg cctgaagcga tcgaagcagc cattacgcca cgcactaaag
      421 ccatcattcc ggtgcattat gccggtgcgc cagcagatat tgacgccatt cgcgccattg
      481 gcgaacgtta cggcatcgca gttatcgaag atgctgccca tgccgtcggt acgtattaca
      541 aagggcgaca tattggcgca aaaggtactg ctattttttc gtttcacgcc atcaaaaata
      601 ttacctgtgc ggaaggtggc ctgattgtta ctgataatga aaatcttgcc cgccagctgc
      661 ggatgctgaa atttcacggt ctgggtgtcg atgcctatga cagacatacc tggggccgtg
      721 caccgcaggc tgaagtctta acgccgggct ataagtacaa tctgaccgat attaacgccg
      781 cgattgccct gacacagtta gtcaaattag agcacctcaa cacccgtcgg cgcgaaattg
      841 cccagcaata tcagcaagca ctggcagctc tcccctttca gccattaagc cttcccgcct
      901 ggccgcacgt tcacgcctgg catctgttta ttattcgtgt cgatgaacaa cgttgtggta
      961 tcagtcgcga tgcgttgatg gaagcgttaa aagaaagagg cattggtacc gggttacatt
     1021 tccgcgccgc tcacacacaa aaatattatc gcgagcgttt tcccacgctg tcgttaccga
     1081 ataccgaatg gaatagtgaa cgcatctgtt cgttgccgct gttcccggat atgaccaccg
     1141 ccgatgccga ccgcgtcatc acagccctta agcaactcgc aggacaataa gccatgtttg
     1201 aaatccaccc tgttaagaaa gtctcggtgg ttattcccgt ttataacgag caggaaagct
     1261 taccggaatt aatcaggcgc accaccgcag cctgtgaatc actggggaaa gagtatgaaa
     1321 tcctgctgat tgatgacggc agtagcgata attccgcgca tatgctggtc gaagcctcac
     1381 aagcggaggg cagccatatt gtgtctatct tgcttaaccg caattacggg caacattcag
     1441 cgattatggc gggattcagt cacgtcaccg gcgacttaat tattaccctc gatgccgatc
     1501 tccagaatcc gccagaggaa atcccccgtc tggtggcaaa agccgatgaa ggttacgacg
     1561 tggtagggac tgtacgccag aaccgccagg acagctggtt tcgtaaaacc gcttcaaaga
     1621 tgattaaccg gcttattcag cgcaccacag gcaaagcgat gggtgactat ggttgtatgc
     1681 tgcgcgccta tcgccgtcat attgtcgatg cgatgttgca ctgccatgaa cgcagcacct
     1741 ttatcccgat tctggcgaat atcttcgccc gccgtgccat tgaaattcca gtgcaccatg
     1801 ccgagcgtga gtttggtgaa tccaaataca gctttatgcg cctgattaat ttgatgtacg
     1861 acctggtgac ctgtcttacc acaacgccgc tacgtatgct gagtctgctc ggcagcatta
     1921 ttgcgattgg cggttttagc attgcggtgc tgctggtgat tttacgcctg accttcggac
     1981 cccaatgggc ggcagaaggc gtctttatgc tatttgccgt gctgtttact tttattggcg
     2041 ctcagtttat cggcatggga ttgctcggtg aatatatcgg caggatctac accgatgtcc
     2101 gcgcccgccc ccgctatttt gttcagcaag ttatccgtcc atccagcaag gaaaatgaat
     2161 aatgaaaacc gtcgtttttg cctaccacga tatgggatgc ctcggtattg aagccctgct
     2221 gtctgccggt tacgaaatta gcgccatttt tacccatact gataaccccg gtgaaaaagc
     2281 cttttatggt tcggttgccc gactggcagc ggaaagaggc attccagttt atgcgccgga
     2341 taacgttaat catccgctgt gggtggaacg cattgcccaa ctatcaccag aggtgatttt
     2401 ctctttttat tatcgccatc ttatttgcga tgagattttg cagctcgctc cgagaggtgc
     2461 atttaatctg catggttcgc tgttaccaaa atatcgtggt cgcgcgccgc tgaactgggt
     2521 gctggtgaac ggtgaaacgg aaactggcgt tacattgcac cgaatggtga aacgtgccga
     2581 tgccggggcc attgtggcac aactgcgcgt tgccattgcg ccagacgata tcgccattac
     2641 gctgcatcat aaattgtgcc atgccgcgcg ccagctactg gaacagacat tacccgccat
     2701 taaacacggt aatattctgg aaatcgccca gcgcgaaaac gaagccacct gttttggtcg
     2761 cagaacgccg gatgacagtt tcctcgaatg gcataagccg gcatccgtac tgcacaacat
     2821 ggtgcgtgcc gttgccgatc cgtggccggg tgccttcagc tatgttggca atcagaaatt
     2881 caccgtctgg tcgtcgcgtg ttcatcctca tgccagcaaa gcacagccgg ggagcgtgat
     2941 ttctgttgca ccactgctga ttgcctgtgg cgatggcgcg ctggaaatcg tcactggaca
     3001 ggcgggcgac ggcattacta tgcagggctc gcaattagcg cagacgctgg gcctggtgca
     3061 aggttcacgc ttgaatagcc agcctgcctg tgccgcccga cgccgtaccc gggtactcat
     3121 cctcggggtg aatggcttta ttggcaacca tctgacagaa cgcctgctgc gcgaagatca
     3181 ttatgaagtt tacggtctgg atattggcag cgatgcgata agccgtttta tgaatcatcc
     3241 gcattttcac tttgtcgaag gcgatatcag tattcattcc gaatggattg agtatcacgt
     3301 caaaaaatgt gatgtcgtct tgcctttggt ggcgatagcc acgccgattg aatatacccg
     3361 caacccgctg cgcgtatttg aactcgattt cgaagagaat ctgcgcatta tccgctactg
     3421 cgtgaagtac cgtaagcgaa tcatcttccc gtcgacttca gaagtttatg ggatgtgtag
     3481 cgataaatac ttcgatgagg accattctaa tttaatcgtc ggcccggtga ataaaccacg
     3541 ctggatttat tcggtgtcta aacaattact tgatcgagtg atctgggcct atggcgaaaa
     3601 agaaggttta cagttcaccc tcttccgccc ctttaactgg atggggccac gactggataa
     3661 ccttaatgca gcgcgaattg gcagctcccg cgctattacg caactcattc tcaatctggt
     3721 agaaggttca ccgattaagc tgattgatgg cggaaaacaa aaacgctgct ttactgatat
     3781 tcgggatggt atcgaggcgt tataccgcat tatcgaaaat gcgggaaatc gctgcgatgg
     3841 cgaaattatc aacattggca atcctgagaa cgaagcgagc attgaagaac tgggtgagat
     3901 gctgctggcg agcttcgaaa aacatccgcc gcgccattac ttcccaccgt ttgcgggctt
     3961 tcgtgttgtc gaaagtagca gctactatgg caaaggatat caggacgtag agcatcgtaa
     4021 accgagcatc cgcaatgccc gccgctgcct ggactgggaa ccgaaaattg atatgcagga
     4081 aaccatcgac gaaacgctgg atttcttcct gcgcaccgtt gatcttacgg ataaaccatc
     4141 atgaccaaag tcggcttacg cattgatgtc gatacctttc gtggcacccg tgaaggcgtg
     4201 ccgcgtctgc tggaaatctt gagtaagcat aatattcagg ccagcatttt tttcagcgtc
     4261 ggcccggaca atatgggccg ccatctctgg cgattggtga agccacagtt tttgtggaag
     4321 atgctgcgct caaacgcggc atcgctttat ggctgggata ttttactggc aggtacggcc
     4381 tggccaggta aagagattgg tcatgccaat gctgatatca ttcgtgaagc ggctaaacat
     4441 cacgaagtcg gcctgcacgc ctgggatcac catgcctggc aagcccatag cggtaattgg
     4501 gatcggcaaa caatgatcga cgatattgca cgcggccttc gcaccctgga agagattatc
     4561 ggtcaaccgg taacctgttc tgccgctgct ggctggcgtg ctgaccaaca ggtgatcgaa
     4621 gcaaaagaag cgttccattt gcgctacaac agcgattgtc gtggggcgat gccgtttcgt
     4681 ccattgctcg aatcaggaac ccctggcact gcgcaaattc cggtgacctt acccacctgg
     4741 gatgaagtga ttggtcggga tgtgaaagca gaagatttta acggttggtt actcaaccgc
     4801 atccagcgag ataaaggcac gccggtttat accattcatg ccgaagttga aggctgcgct
     4861 tatcagcata attttgtgga tctcctgaaa cgcgcagctc aggaaggcgt gacattttgc
     4921 cctttaagcg aactgttatc agggacgttg ccgctcggac aagttgttcg cggaaatatt
     4981 gccggacgtg aaggctggct gggttgccaa caaattgcgg gtagtcactg atgaaatcgg
     5041 tacgttacct tatcggcctc ttcgcattta ttgcctgcta ttacctgtta ccgatcagca
     5101 cgcgtctgct ctggcaacca gatgaaacgc gttatgcgga aatcagtcgg gaaatgctgg
     5161 catccggcga ctggattgtt ccccatctgt taggactacg ttatttcgaa aaacccattg
     5221 ccggatactg gattaacagc attgggcaat ggctatttgg cgcgaataac tttggtgtgc
     5281 gggcaggcgt tatctttgcg accctgttaa ctgccgcgct ggtgacctgg tttactctgc
     5341 gcttatggcg cgataaacgt ctggctttac tcgccacagt aatttatctc tcattgttta
     5401 ttgtctatgc catcggcact tatgccgtgc tcgatccgtt tattgccttc tggctggtgg
     5461 cgggaatgtg cagcttctgg ctggcaatgc aggcacagac gtggaaaggc aaaagcgcag
     5521 gatttttact gctgggaatc acctgcggca tgggggtgat gaccaaaggt tttctcgccc
     5581 ttgccgtgcc ggtattaagc gtgctgccat gggtagcaac gcaaaaacgc tggaaagatc
     5641 tctttattta cggctggctg gcggttatca gttgcgtact gacggttctc ccctggggac
     5701 tggcgatagc gcagcgggag cctgacttct ggcactattt tttctgggtt gagcatattc
     5761 aacgctttgc actggatgat gcccaacata gagcgccgtt ctggtactac ttgccagtca
     5821 tcattgccgg tagcctgccg tggctgggat tactccccgg tgcactgtac gctggctgga
     5881 aaaaccgcaa gcattccgca accgtctatt tgttgagctg gacgataatg ccgctgctgt
     5941 ttttctccgt cgctaaaggc aaattgccca cctatattct ttcctgcttt gcacctctgg
     6001 caatgctgat ggcgcattac gcgttgctgg cagcaaaaaa taatcctctg gcgttgcgga
     6061 ttaatggctg gattaacatc gcttttggcg tcactggcat tattgccaca tttgtggtct
     6121 ccccctgggg accaatgaac acaccggtgt ggcaaacctt cgagagctat aaagtctttt
     6181 gtgcctggtc tattttttcg ctatgggcat ttttcggctg gtacacctta acaaacgtcg
     6241 aaaagacctg gccttttgcc gcgctttgcc cgctggggct ggcgttgctg gtaggatttt
     6301 caattcctga cagagtcatg gaaggaaaac atccgcaatt ttttgtcgag atgacacaag
     6361 aatcacttca gccaagccgc tatattctta ctgatagcgt cggtgttgcc gcaggtctgg
     6421 catggagcct gcaacgcgat gacatcatca tgtatcgcca gacaggtgag ttgaaatatg
     6481 gccttaatta tccggatgca aaagggagat ttgtcagcgg tgatgagttc gcaaactggc
     6541 ttaatcaaca tcgtcaggag gggattatta cactcgtgct ttcggttgac cgcgatgaag
     6601 atatcaatag tctcgccatt ccgcccgcag atgtcatcga tcgtcaggag cgtctggtgc
     6661 taattcagta tcggcccaaa tgatctggct aacattagtc tttgccagct tgcttagcgt
     6721 tgccgggcag ttgtgtcaga aacaggcaac ctgctttgcg gcggtaaaca aacggcgcaa
     6781 acatatcgtg ctgtggctgg gactggcgct ggcttgtctt ggccttgcca tggtgctctg
     6841 gctgctggtc ttgcagaacg taccggtagg cattgcctac ccgatgttaa gtctgaattt
     6901 tgtctgggta acgctggctg cagtaaaact gtggcacgag ccggtatcgc tgcgtcactg
     6961 gtgtgggttg gcgttcatta ttggcggcat tgtgatcctc gggagtacgg tgtaatgggc
     7021 ctgatgtggg gattattcag cgtcattatc gcctccgctg cgcaattaag cctgggtttt
     7081 gcggcgagtc atctgccgcc aatgacgcac ttatgggatt ttatcgcggc gctgttagct
     7141 tttggcctgg atgcccggat actgttactc gggctgctgg gttatctgct ttccgttttt
     7201 tgttggtata aaacactaca taaacttgcg ctaagcaaag cctatgcctt gctcagtatg
     7261 agttatgtgc tggtatggat tgcttcaatg gtacttcccg gctgggaagg gactttttcg
     7321 ctgaaagcac tactgggagt agcctgtatt atgagcgggt tgatgctgat tttcctgccc
     7381 acaacaaaac aacgttactg agttttccct gccgctttac actgccgttc ccactcatcg
     7441 gggctataac agcttgcaga aagaactttc accgtgtgca gcttttcacg attaatacag
     7501 taaagcgcat tctgcaaagg cgagagtaaa tcccccactt tcacggcgaa gtcactcttc
     7561 acttcggcga tcatttttat cgcgccgcca gcatcgcaga gcataattac atgtctgtta
     7621 tcttgtttat tgcagcacgt ttttttgacc agccattcca ttgcattatc ctgtttgcta
     7681 agagttttca catcaattta cagattaaca gataaactct ttatcaacgg caatggaatg
     7741 gcagaaatga gaagtgaaac ggagggttaa ttattgctgg cgttgcaccc actcttttag
     7801 cgcctgacgt gaaattttaa taccgccgtt tttcagctcc ggcggcagag tcagccagcg
     7861 caccggttgt tgaaaacgag ccagcttatc tttcacccat tcgtcaagat caacgctttg
     7921 ctggtcatac tccaccaccg ctaccggccg atgaccaaac tccttgtcgg caacgggaac
     7981 gataaacacc tgcaaaaccg caggatgtgc agcaataaca cgctcgactt cttccggctg
     8041 aataccctca ccgccactga agaacagatt atctaaacgt ccgacaatgg tcagcttgcc
     8101 attatgcatc tcaccgcgat cgcgcgtggc gtaccagcct tcgtcattaa ccagtggaac
     8161 ccgttgcccg ttacgccagt aaccttctgc catactggca gcccgcagcc acacttcatc
     8221 attaacgatt ttcacttccc gacccggcag cggcgaacca acgtctgcca gaccgtcggc
     8281 ttctttcgca cacactgtgg aggcaaactc ggtcagacca tagccgcaaa agcaccgaat
     8341 cccctgctcg cgcgcctgtt ccgtcaactc gaccgggata gccgcgccgc caagtaaaac
     8401 cgctttcagg gaaacggaac tacggttaac cagcaaacgc cagagttgtg ttggcaccag
     8461 tgaagcgtga gtacagcctg ccagcatctg ctccaatggc tgtttatcac gcaccgtcat
     8521 ccgcgcacca gcgtataacc agcgccacat aattccctga ccggagacgt gaaacagcgg
     8581 taaagagagc aaccagtcat cgtgatcgcc aaacggaatc agcgataaca ccccttcggc
     8641 gctgacaaga tgagcctgat aagtatgaac agcggctttc ggcaagccag tggaaccaga
     8701 ggttaacgtc attgagcaca gacgcgtcgg ctgccacgcc gcggcatgtg cgccttcaac
     8761 cagctgaatg tgcagcgacg ctaacgccgg aaacgtgttt tccccttccg gcaccagcgc
     8821 aaattgcagc gtcagattgg gcagcaattc ctcaagcagc ggttgcggca gctgagggtt
     8881 cacgggcaac acccgcgccc cgcattgcag taacgccagc caggccagca gcgtttgcgg
     8941 cgtattccac gcccgcaaca tcacgccgct gccctcaacc acaccctgca ccgcaaagct
     9001 ggaggctaat tcatcaacgc gagcacaaag ctcgcgccag ttgagttgct cgtcattgag
     9061 acgtaaggcg atggcttctc cccgcacttg ccgccagtga cgccacggcc agtcagagaa
     9121 gatcataaca gccgctccag tgcatcaacg tccactaacg gcagtgggct ccccggccag
     9181 cgacgaacct gctgcgcctg catcagatcc agcgtgtcca gtcccggaat ggtgtctggc
     9241 gttaaccagg cagcaatccg cgccagttgc gttagaccta agctcgactc aatggaagaa
     9301 ctgatcaccg ccgtcagccc cagcgcatgc gccgcctgca cttgctcgcg tactttatcc
     9361 aggctgcccg tgagcgtggg cttgataacc accgcgcgca cgccctcttc agctacaaag
     9421 gcaaaatctg gctcgcgcag gctttcatcc caggcaatgg caatgccggt ttcacgggca
     9481 aacgcacgcg aatcatcgcg ggttttgcac ggctcttcga gaaacgcgat gcggtggcga
     9541 taatccgggt taacgtattt ggcaaactgc tggcctttca gcggtatcca ggcgcgattc
     9601 gcgtcaagac gcaaatgcag atcaggaatt gcctccagca acagattcac caccatgccg
     9661 tcgcgcacgg cttcgtacaa tcccactttg acctttgcca ctttctcgcc aggcatatcg
     9721 gcaagtttga ggatcagatc gtcaggatcg ccattacaca gcggtgccgc acggtaattg
     9781 gccgcctgcg ggagcgtatc tgccagctcc gccaatgcac aacttacgcc aaaggcaacg
     9841 gaaggcatct gcggtaattc gcaatcgcct gccagccagt tatttaccca ggcaagcagc
     9901 acactttgcg catcttccca ggtttcctga ctgaagcccg gcagtgggga gatctccccc
     9961 cacccttcgc gctcgccctc acgcaggcaa acatacagcc cgtcgcgggt ttttaaccgc
    10021 ctgtcgcgca gaaccacccc cgcgtccatg gggatctgcc agcggtatac ctgcgcgcta
    10081 cgcattacgg attccgttta aatttgctga aatcaggctg acgtttctgg ttgaaagcgt
    10141 tgcgaccttc ctgaccttct tccgtcatat agaacagcat ggtggcgttg cccgccagct
    10201 cctgcagacc tgcctgaccg tcacagtcgg cattcagcgc agctttcagg cagcgcagtg
    10261 ccatcgggct gttttgcaac atttcacggc accagcgcac ggtctctttt tccagatccg
    10321 ccagcggtac aacggtgttc accaggccca tatccagcgc ctgttttgcg tcgtactgac
    10381 ggcacaggaa ccagatttca cgtgcttttt tctgcccaac aatgcgcgcc atataggaag
    10441 cgccccagcc gccgtcgaag gaaccgactt tcgggccagt ctgaccgaag atggcattat
    10501 cagccgcgat agtcaggtcg cacatcatgt gcaggacgtg accgccaccg atggagtagc
    10561 cagccaccat cgcgacaacc ggttttggac aggtacggat ctgacgctgg aagtccagca
    10621 cgttcaggtg atgtacgccg gaatcatctt tatagccgcc gtaatcacca cgtactttct
    10681 ggtcaccacc ggagcagaac gctttatcgc ctgcaccagt cagaatgatc acgccgatgt
    10741 tgtcgtcata acgcgcatcc gccagcgcct ggatcatctc ttttaccgtc agaggacgga
    10801 aggcattgcg cacctgcgga cgattaatgg tgatttttgc gataccgtcg gtggattttt
    10861 cataacgaat gtcctcaaaa ccttcggagc agtcgtgcca ttcaaccggt gcgtaaagca
    10921 ttgcttcatc aggataaatc atattgtgtc ctttagtcag aaacgcaaga tctgcgccag
    10981 acttgcgatt acgccagcgg gattttcccg gtgcgcgtta tgtccggcgc gaggaatgac
    11041 atggcagtcg gcagccagtt ccgccgccag agcgcggaat ttgctgtcac gttcaccaca
    11101 taaataataa aacgcaaatg tgcgggcgct aaggttagca cgtaaatcgg gctggacggc
    11161 gagagaagtc gcctccagca tggcggcaag ggttgcgcca ttattgttgc tgcgcagcgc
    11221 caccagctcc cggcgttgat cgtcattgag cgaggcaaaa acaggctgtt gataccaatc
    11281 ggcaaatacc gccgttaacg gttctgtacg aaaacgctgc gcccattggc gatcggaacg
    11341 ctgacgttcc gcgcgttgtt cagcattttg caatccagga tgcccgcctt cgacaacaac
    11401 tccacaaagc cccgccagct cctggcaagc cgccatcatc gccacccgtc cgccaagcga
    11461 gtaccccacc agccagaagt taaggatgtt gtaactaacc agcgttttac acaataagcc
    11521 ggtgacatca tcaaatccat cgacgctgat cgtcgctgaa ccaccgtgac ctgggagatc
    11581 aacatacaac cgtgagtagt cggcaaacac ctcgcccact tcttgccatt catggcaatc
    11641 gccggaaaaa ccgtgcaaaa acaccagcca gggtaaacct ggtttaccgt gttttgcctg
    11701 cgcgtgcagg atcataaatg gcttacctgc gccagcagtt gctggagggt ttgcgcacca
    11761 tcggtgtcgt taaccaccat ttcaattacc gtggtggtcg gcgtgcgcca ggcgtcggca
    11821 agtgccgttt caagttcctg ccagttttgc ggacgatgat atttcagctc gaacatcgcg
    11881 gcggcgtgct caaaatggac gttttgcggc atcagataga aacgctcgcg ctcgtttttc
    11941 ggcgttggca acagcgagaa aatttgcccg ccgttgttgt tcaccacaat taataccagc
    12001 ggcgcggaaa cctgacgtaa taacgccagc gcgttgagat cgtaaagtgc ggagagatcg
    12061 cccacaatcg ccagcgtcgg tttgccgctt gcccgctgaa cgccggcggc agtcgagagc
    12121 agcccgtcga taccgctggc accacggttg ctgtacaccg ggtaacctgc cggaagttgc
    12181 gaaagcgcat caatcagacg caccaccagg ctgttaccga caaacaattg cccctgttca
    12241 ggcaagtagt cgctgatgcg atgcgccagt tgcgcttcgc caaacgcatc gcgacgggca
    12301 ataaccgcct gcattgcctg ttccgccagg cgcgggattt caacgcacca gggctggcgt
    12361 ttttctgccg gatgcagctc cagccagtcg gcaatattgg caattaagcg acgtccgcga
    12421 tggtgtgccg gatcaagtcg cccttcaatg tcatcaacaa tccagtactc ttctggttca
    12481 cagcttgcct gccattggag gagtcgtttc cccgtcaggc tgcttcccag ttgcaccact
    12541 atttgtgcct gttgcagttc gctggtcgct ttggcattgc ctaaccagag atcggcacac
    12601 ggcagcggct gtccggtttg cgacagcacg tcgccaatca gcggccagcc aagagtttgc
    12661 gcccacagtg caactttttt gccctcttcc gcactcatgc gcccggcaac caccacgccg
    12721 cgcttttgtc gccagaagaa ccagtcacgc tgtttttcac tttcccgacg aggcgcttca
    12781 cgcagccacg gtttgtcgtc ctgccaccag tcgcccagcc gctgttgcca gctaatcccg
    12841 gtgtcgtcca tttcgccata cagcggttca gcaaacgggc agttgatatg gactccacca
    12901 gcatgaagcg taccgagagc gtggtcgatg gtagaaacca gccaacgtgc ggggatatcc
    12961 tgggtcgggc gcggcagtga aatactgtgc gtggggtgag aggcgaacat tcccggctga
    13021 cgaatcgcct gattcgcgcc gcagtcaatc agctccggcg ggcgatcggc ggttaacagg
    13081 atcagttttt ctccggttaa cccagcttca atcagtgccg gatagagatt tgctaccgcc
    13141 gtgccggagg tcacaatcac cgccaccggc tgcttgctga ctttcgccag ccccagtgcc
    13201 agatgcccca gtccacgctc atcgaaatgg gtatgatgaa tgaatgcgga attctccgcc
    13261 gccgctaacg ttaacggtgt agaacgcgag ccaggggcga tacagatgtg tctgacgccg
    13321 tgacgcgtta atgcttccag aatgaccgcc gcccagcgtc ggttaaatgc gcttactgac
    13381 atgagtttgt ccggtatcaa tattgcggct aagtataagg agctacaaaa atagattatt
    13441 gatatgaatc ggtaatgatg cgactcatta ctattccatt tgtaataaag tacgcagccc
    13501 tgccgctttg ttgtcgattt cctgccactc ttgctcgggg tcggaaccac ggacaatgcc
    13561 tgcgccagca tatagccgca cgatattgcc gctgattttt gctgagcgca gggaaacgca
    13621 gaattcactt tgctgtaatg agagatagcc cgcagaaccg gcgtaccatt ctcgagtgaa
    13681 cggttcgtga cgggcgataa actgtcgcgc cagatcgcgc ggtaagccag caactgctgc
    13741 cgttggctgc aactgatgta aacagatcac atcatccgct ttgttgagtg aagtccagat
    13801 acagcggcga agatgctgta ctttacgtag acgcagtacc tgcggcggta aaacatccag
    13861 cgtttgggta tcggcctgca atcgttggca gatatcttcc accaccaaca tgttctcgcg
    13921 ctggttttta tcatccgcca tcagccactc acctaactgc tgcgcctgct tatcatcagg
    13981 atgatttgct accgttcccg ccagcgcttc ggtacgcagc gctttgtcac gccgccgcca
    14041 taaccgttcc ggtgaagaac caagaaaagc attttcgcca tcaaaggcca tgtaaaaatg
    14101 gtagcaattc agattgagcc gacgactagc agccatcatc gctgccgcat tgaccggact
    14161 tgcgaaatgc aggtcagttg cccgagcaag cacaactttg tcgagctcgc cttcggcgat
    14221 ggttttcgtt gccagctcga ttaattgcgt ccagcccgtt ttgtccggcc agtgttgttc
    14281 tcgcgtggtg gttaaatgta atccaggcag gggcttgata ctcaccagtg tggcgataaa
    14341 ttcttttgcc ttgattgcat cgtgctgaag ggagctttcg ctgaatagcg tcagccgcag
    14401 cgtggcttta ccgccacaac gtcgccattc caggcgtggt aaaagtaaat tgccctgcga
    14461 cgggtcaaac gcattcaacc cccagatgcg taagtcggcg tgttccgggt gctggcgaag
    14521 aaaacgttgt gcctggtcca acgacgtaaa acgggtaatc gcacccagga cggcagcttc
    14581 ttcatcacca ttacgttgtt gccagtagaa ttgcggatac acctgctgac tggccagcca
    14641 gctcaaggca tcaaaagcgt ctttgagagg gaaaggaata tcgataaccc gaatgccggg
    14701 tgtcgccgga atctcttgcg acaaatggcg cagtagattt tccagcgccg tagtaagtga
    14761 ttgcacgcgg acctctccct gttaaaagcc tcacattata cggggtacga caaaaaaaat
    14821 gcagtacccc ggtgtaggga ggttttaacg gcgtgccagc aacagtccta gaaccagccc
    14881 aacggccgca cccacaccaa ttccttgcca gggtttttcg tggacgtagt catcagcacg
    14941 ataaacagcc tgcttcgccc gatagtaata actgtctgaa gcctgactaa cccgtttttt
    15001 cacatcatcc agcgcttttt ctgcacgcgc tttcagctca acatatttct gatcggcggg
    15061 atcgccagag gagcggagca cctcttccag tgtttcacta agcagcgtca ggtcgtcatc
    15121 aatacgtgta tcaccaaact gattagacat tctcgttctc catttgtgta aaacctgtca
    15181 ctaactatag acaagggtta ttgattacgc ctgaattacc tcgcgtgcca tcccaatgtg
    15241 tgggatacca tcttcttcat aaatatcggt cacagggatg aaaccaaatt gcccataaaa
    15301 gttttgtaaa tgcgcctgcg cccccagata aatcggcttc tccggccagc ggcgagtaca
    15361 cgattccagt gccttactca tcaattgctg accgattttt tcaccgcgta aggcttcgct
    15421 gacaatcacc cgaccaatca caacgggttg cagttcgtcg tcacttttca gaatcctcgc
    15481 atacgccacc agtgtcccgt tatgccaacc gagaatgtgg cgattttctc cctccagatc
    15541 gtcaccatcg atatcctggt aaggacagtt ttgttccact acaaataccg cacaacgcag
    15601 ttgtaataag gcataaagct gagaaacgga taaatcagaa tgatgcagat cttgccagtc
    15661 gatcatggaa ggctcctttg tgctgtttgc tttatactag ccggaatatt ttcagcagcg
    15721 tgatgaactc atggaattaa tttttttagg tacttcagcc ggtgtgccaa cccgtacgcg
    15781 taatgtcacg gcaatattgc ttaacctgca acatccgacc cagagcggac tttggttatt
    15841 tgactgcggt gaaggcaccc agcatcagct actgcatacc gcctttaacc ctggaaaact
    15901 ggacaagatt tttatcagtc accttcatgg cgatcatctc tttggtttac ccggcttgct
    15961 gtgcagtcgt tctatgtcag gcattatcca acccttaacg atttatggtc cgcacggtat
    16021 ccgtgaattt gtggagaccg cgctgcggat tagcggctca tggaccgatt atccgctgga
    16081 aattgtcgaa attggcgctg gcgaaatttt cgatgatggc ctgcgcaaag tgaccgctta
    16141 tccgatggaa cacccactgg aatgttatgg ttatcgtatt gaagaacatg ataaactagg
    16201 tgcattaaat gcccaggcat taaaagctgc tggcgtgccg cctggcccgc tgtttcagga
    16261 attaaaagcg ggcaaaacaa tcatgctgga tgatggaagg cagattaatg gcgcagatta
    16321 cttagccgtt ccagtgccgg gtaaagcgct cgctattttc ggcgataccg gcccctgcga
    16381 tgccgcgctg gagctggcta aaggcgtcga cgtaatggtt cacgaagcga ctctggatat
    16441 ggcgatggag gccaaagcca acagtcgcgg ccatagttct acacgccagg cggcggcact
    16501 agcccgtgag gctggagtcg gtaagctaat cattacccac gtcagctcgc gctatgatga
    16561 caaaggttgt cagcacctgt tacgtgaatg tcggtcaatt ttcccggcga ctgaactggc
    16621 gaatgatttc gcggtgttta gcatttaagt cgaaagcggt ggttagttag cagattaatt
    16681 taacgatacc aacgcattta tttgacattt tttcatctgt ttgccggata cgtttgatgt
    16741 cgatatcagg caaacagaat tatcattttt agttttgtga attgtccaga tctttcgcca
    16801 gtccaatcag gcgaataaat tccgctctgt aaccctgtgg atcttcccct tttgccttct
    16861 gagcccactg tttgatctgc tgccaggagg tattattcag gtattcagaa ccgcgtaact
    16921 tttgcccata tgcagccact gctgcgcgaa aacgcatatc ttctgaaggt gcctttattg
    16981 cgaatgccaa cgggaattca actaactgac tttcttttcc ctgtggggat ttccaacgaa
    17041 tctttaacca ggcaagttct tttgttttgt ccgatttcgc tgatttgtta tccggggcat
    17101 agcgtaactt atcaattgat gctttttgcc ctttcagcgt taattcaaat aacaatgtta
    17161 tatgtttacc tgcgccaata tcacccgcat caacgttgtc gttattaaaa tcctctgccc
    17221 taagttggcg cttttcataa cctatctgac ggtattccgt tacccacgcg gggttaaact
    17281 caatttgcgc tttgacatct tttgctacgg taatcaacgt ctgccgcatt tcactgttca
    17341 acactttctg cgcctctgag agggtatcaa tgtagctata gttgccgtta ccaacatcgg
    17401 caattcttac catcattgcc tcgttataat tgctatcccc cacgccaagc gtcgacagag
    17461 taacaccgga ctcacgctgt tttttgacca ttgattcaat cgattttgga tcgtcaatgc
    17521 caacgttaaa gtcaccgtca gtggccaata aaatgcgatt gataccacct ttaataaacc
    17581 ccttcgctgc ctgctgataa gccatctcca gcccggcacc gccattggta ctgccttctg
    17641 catccagcga atcaattgca gcattaattt ccgccttgtg actaccggag atagaaggca
    17701 acgcaatacg agagtcgcca gcgtaggtca cgatggcaat gttatcctgc tcacgaagtt
    17761 ctttgaccaa taatttcaac gaagactgga taagtggcaa acgttcatca gaaatcattg
    17821 aaccagaagt gtcgataaga aagaccagat tagaagctgg taactcttta cttttgcgat
    17881 ctttcgccag gatatcaact ttcagcaatg ttcgctgttc attccagggt gcaggtgcca
    17941 attcgtagcg catagcgaaa ggtattggct tagaggccgg aatagattgt ttgtctttga
    18001 tatcccagtc agacgggaaa taattgacta cctcctccac ccgcacagcg tctggcggag
    18061 gtaacagccc ttgattaagg aaacgccgca cattcgcata actgccggtg tcgacgtcaa
    18121 ggctaaacgt cacgagtggg ttttgcgcta cctgcttaac cggattatca tcgaactgct
    18181 ggtagcgagc ggttcctgga tttgcgatat gtgtagcgtt tgctttagcc gctcgtgcaa
    18241 atgttggcgc ttcctgcaat ctcccctgca aagtctgttt gtctgaatat tgttgcactt
    18301 cttgctgggc taaggctttc gcgtctgcag cagctttagc ggcggcggcg ctttgctcta
    18361 cctcttttat tgcagcttgt tgcgcgacaa gcacttgttg gtctgagggg gtactgggtt
    18421 gttgttgctg actttcctta tcctcaggtt ccggcccaca tcctga
//
LOCUS       DAZFFE010000095        18200 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZFFE010000095 DAZFFE010000000
VERSION     DAZFFE010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.095"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(21..251)
                     /locus_tag="ACHJ69_003581"
     CDS             complement(21..251)
                     /locus_tag="ACHJ69_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016488.1"
                     /translation="MTPGRREDKCPSARAGKRPRAGGAERGRAYAPPLSLRGRSAVGF
                     CLFRPVICLLLMMLLFLLLLLLLLLFFFFLLL"
     gene            complement(251..813)
                     /locus_tag="ACHJ69_003582"
                     /pseudo
     CDS             complement(251..813)
                     /locus_tag="ACHJ69_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
     gene            complement(860..2221)
                     /locus_tag="ACHJ69_003583"
     CDS             complement(860..2221)
                     /locus_tag="ACHJ69_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFO5016489.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPEQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAETEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(2273..2503)
                     /locus_tag="ACHJ69_003584"
     CDS             complement(2273..2503)
                     /locus_tag="ACHJ69_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016490.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFRRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            3021..3492
                     /locus_tag="ACHJ69_003585"
                     /pseudo
     CDS             3021..3492
                     /locus_tag="ACHJ69_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311849.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3471..3662)
                     /locus_tag="ACHJ69_003586"
     CDS             complement(3471..3662)
                     /locus_tag="ACHJ69_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016491.1"
                     /translation="MNISKETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVV
                     NQQAVFLGGHYILQGSRHR"
     gene            complement(3662..4081)
                     /locus_tag="ACHJ69_003587"
     CDS             complement(3662..4081)
                     /locus_tag="ACHJ69_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO5016492.1"
                     /translation="MYCTVKEIIREVLDTDVPNSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEHGADVSIVHDVVRELMEEKRVNRLVTVPAVLLEKVMVMAGSEI
                     YRLHAVGSENGGDGDAFVREEREIMRVMRQALDGENG"
     gene            complement(4128..4553)
                     /locus_tag="ACHJ69_003588"
     CDS             complement(4128..4553)
                     /locus_tag="ACHJ69_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5016493.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            complement(4967..5737)
                     /locus_tag="ACHJ69_003589"
     CDS             complement(4967..5737)
                     /locus_tag="ACHJ69_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016494.1"
                     /translation="MNKTLNALVCHHARNLLLAQGWPEETDIEQLNPHYPGWISIYVR
                     LDVSRLATLLISRHDGVLPPVLASAMQKLTGTVAELVLSGSQWQALPVLPADGTQVFF
                     PYAGEWLTEEEIRAVLDAVRNAVRSICYQVADDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDYPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVCECVEHILASGLVCDVLR
                     IPDEPSRRWFDRDILREVVLEARDEIRSMADALAKIRG"
     gene            complement(5782..6300)
                     /locus_tag="ACHJ69_003590"
     CDS             complement(5782..6300)
                     /locus_tag="ACHJ69_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO5016495.1"
                     /translation="MAPIACSWRRIPKFWNWMRASKIEKEMRMYGTCETLCRELAAKY
                     PGDTPLMLVIWSPEEIQALADGMDISLSDHEIRTVLARLEDIPEDQRTESGISSGVAM
                     EIISNVSENRQVTVPAELLASLIQTAEQALWKREWAARDYGLAVPECVTRRQAVVNQA
                     RILLKNNTHEND"
     gene            complement(6230..6451)
                     /locus_tag="ACHJ69_003591"
     CDS             complement(6230..6451)
                     /locus_tag="ACHJ69_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016496.1"
                     /translation="MNYAGHEKLRAEVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDACFKD"
     gene            complement(6452..7135)
                     /locus_tag="ACHJ69_003592"
     CDS             complement(6452..7135)
                     /locus_tag="ACHJ69_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO5016497.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DVNDDWLQPASNEMYRVLKKNALMVSFYGWNRIDRFMAAWKRAGFSVVGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            complement(7211..7522)
                     /locus_tag="ACHJ69_003593"
     CDS             complement(7211..7522)
                     /locus_tag="ACHJ69_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016498.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARQERCLWSVSRV
                     RRARDGRFYCSRLASVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(7519..8047)
                     /locus_tag="ACHJ69_003594"
                     /pseudo
     CDS             complement(7519..8047)
                     /locus_tag="ACHJ69_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134314.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
     gene            8049..8132
                     /locus_tag="ACHJ69_003595"
                     /pseudo
     CDS             8049..8132
                     /locus_tag="ACHJ69_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
     gene            complement(8262..9038)
                     /locus_tag="ACHJ69_003596"
                     /pseudo
     CDS             complement(8262..9038)
                     /locus_tag="ACHJ69_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
     gene            complement(9272..10234)
                     /locus_tag="ACHJ69_003597"
     CDS             complement(9272..10234)
                     /locus_tag="ACHJ69_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO5016499.1"
                     /translation="MKKVLARGRVLGNSNSEFARMLEGDGEIKTFTLKSGKQAKFIKT
                     VVLSGDIESKTFVDASVNGRDQTMLSRESVSDITRTIKLQQFFPAIGREVDGRIEVLD
                     GTRRRAACIYNNVKFEILVTKDSIDIADARQLAKDIQTAREHSLRELGKRLEVTYGVS
                     MTKEEIAQAESISPAKVTRAFQAASVPDEMISVFPVINDISLSDYQFLLKLSEKVTNK
                     KMSVEEFMDRVRQKLMALPDYPTIEKSKILAVFRSEGKLLTDRPVKTVETEKLKEFSD
                     RNQFARKKTDPRKRLVVYEFSRISNEVQLEIDEAIKNILRKLPE"
     gene            complement(10231..11436)
                     /locus_tag="ACHJ69_003598"
     CDS             complement(10231..11436)
                     /locus_tag="ACHJ69_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5016500.1"
                     /translation="MKRDYGSVGTIAFRASALLQAMSRDIEEQRKEFNLTEYHQTYTR
                     NAVAKLPKLSRRIVELAVKEMEESGYEFNKKRVGNVEQYALTIQNVIDIYAHRQIPKY
                     RDVHKEPYVIFVVNLKGGVSKTVSTVTLAHALRVHPDLLRHDLRILVIDLDPQASSTM
                     FLDHTHSIGTVLETAAQAMLNNLDAETLREAVIRPTMIPGVDVIPASIDDGFVASQWE
                     SLVAEHLPGFKPSEVLRKTIIDRIADDYDFVFIDTGPHLDPFLLNGLAASDLLLTPTP
                     PAQVDFHSTLKYLTRLPEMLERLEEEGVEPRLSASIGFMSKMTSKRDHETSHSLAREV
                     YASNILDSSLPRLDGFERCGESFDTIISANPVSYPGSAEALKKARTEAERFTKAVFDR
                     IEYIRGASK"
     gene            11810..12021
                     /locus_tag="ACHJ69_003599"
                     /pseudo
     CDS             11810..12021
                     /locus_tag="ACHJ69_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399715.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12194..12445
                     /locus_tag="ACHJ69_003600"
     CDS             12194..12445
                     /locus_tag="ACHJ69_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO5016501.1"
                     /translation="MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYC
                     VPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLDKYL"
     gene            12442..12729
                     /locus_tag="ACHJ69_003601"
     CDS             12442..12729
                     /locus_tag="ACHJ69_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5016502.1"
                     /translation="MRYQVKFREDALKEWQKMDKTIQQQFAKKLKKCCDNPHIPSAKL
                     RGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGKRERSDVYNLASERMR"
     gene            12731..13142
                     /locus_tag="ACHJ69_003602"
                     /pseudo
     CDS             12731..13142
                     /locus_tag="ACHJ69_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558235.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            13786..14763
                     /locus_tag="ACHJ69_003603"
     CDS             13786..14763
                     /locus_tag="ACHJ69_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO5016503.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENQLPPSPAEKISPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(15006..15746)
                     /locus_tag="ACHJ69_003604"
     CDS             complement(15006..15746)
                     /locus_tag="ACHJ69_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5016504.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWPVTDETMRNWLKQAV
                     KRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLK"
     gene            complement(15867..16055)
                     /locus_tag="ACHJ69_003605"
     CDS             complement(15867..16055)
                     /locus_tag="ACHJ69_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFO5016505.1"
                     /translation="MASDTFSCRRRLSAGFFSVSACSWHYPNSPKNGKPALSPHHDWL
                     KTGSLRQNAPLRIWFYPQ"
     gene            16422..17591
                     /locus_tag="ACHJ69_003606"
     CDS             16422..17591
                     /locus_tag="ACHJ69_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016506.1"
                     /translation="MNQKWKTLIVSVLTPSSIISGIALIVLWGYFSRLDRLDIFFDVM
                     NIKSILVLVCCTTILSLIMIIFIFFITSFFIPIVIPYDIKNLPAYNKIQGNFLSVMML
                     SGLFPMAFIYVLYCAFDFDQSVKDNSGWLSILSIGVLIVVISAIINTRYLEYDLSFKN
                     NRMKLLRRVQIYLIIPLSIALLVHLQVIPLEMVFRNIETSDKSINFKVIAELAFMSYF
                     IFILTILPGLIYLKMNPQHKLSKRISSAFVASLMILLVISTQITVLPVIFTHSVIKLS
                     GISDFKIHSYIIKTSEYPEEFFSNAVWGKKNIKPGEYYSVQAVSMFTTNQFILLCPKD
                     IIRFYRESWKFDLLNVDFDTNTRKKLQEEAAYCVPISAISVKRWDMPLQGSKPSN"
     gene            complement(17817..17992)
                     /locus_tag="ACHJ69_003607"
                     /pseudo
     CDS             complement(17817..17992)
                     /locus_tag="ACHJ69_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         4511 a         4713 c         4468 g         4508 t
ORIGIN      
        1 aacaacaaaa cagacagtaa tcagagcagc agaaaaaaga agaacagtag cagtagcagt
       61 agcagtagca ggaacaacaa catcatcagt aacaggcaaa tgacaggtct gaaaagacag
      121 aagcccacag cagaacgtcc gcgtaatgaa aggggcggcg cgtaagcgcg gcccctttca
      181 gcgccaccgg cgcgcggtct tttcccggcg cgggcagacg ggcatttatc ctcccgcctg
      241 cccggagtca tcaggcacgc agacgaataa tcatcgtcgg aacacgggta taagcaaatg
      301 tgccgcacgg cagcacctcg cggacatcag aaaacggtaa cagcttctcc tgctggcgcg
      361 gcccgttcag acagacggca accagcacac cacccggacg caacagggaa aaagcccgca
      421 ggatatgccg gatatcctgc ccgtgactga acggcggatt catgataatc cggctgtaat
      481 actgcaccgg ctgccattcc atgaagtcgc cacactgaac cctgacaccg ttaaaattat
      541 cccgcagata ccggaccagc ccgctgttaa tctccaccgc atcacacatg gcccccggcg
      601 cgacatgacg aatcgcccgc agtatggccc ggtgccggca gagggttcca gaatgtgatc
      661 acggttgctg atctccgcca gcgtcaccag gcgatcacag accggggcag gagtcacaaa
      721 aagctgagat tcagcccgga tttcagcaac cggcgcggcg ttatccagcc ttgcgcgcat
      781 cagccggata cgttctttta actgactgtg catatcatta tctccgtcat cccccgggga
      841 tttcccgggg ggatatacct tatttctgcg ggatttccgt gattttcatg tcggtgatat
      901 acacattcac caggcggaaa ttattgtcca ttgtgcggcg gtagcggtat gccccgtggt
      961 cttctgtctc tgccacactg cgcacggcct tacagtcccg gggcagggcg gcccactgcg
     1021 cctttgtcat ttcccggaaa ccttcccccg gatagttgac taccggcgga cgcttcgcgg
     1081 cctggctggc gacggcagcc tcttctgccg atggtgcttt gtagtccgtg atgcgatcgg
     1141 gcgtcacttt catcgtgccg ctgtacccga gaaaactgta attcggcgtt gtgactgaac
     1201 tcaccgcccc gttgcttttg ttcacgcgga tgatggtcag ccactcgccc cggctgaaaa
     1261 cctgtccgcc cggctcaaaa tcctgtgtcc gggtaaccac gccgccgctt tcgtccagca
     1321 tggcacgctc atagatcagg cggttctgat agtggttaac ccagcgttgc tgatgctgaa
     1381 tctgccgttc atgacagcgg atcgcaattt cgcgggcctg ctccgtggtg atgatgtcat
     1441 catccagggc cgaccataac gatcgacttc cttcatactg gcttttttct gccgggcgcg
     1501 gatacgtatc cagcgggaaa caggcgctga tatggtcatg actgctgatg agttttgcca
     1561 tattcagatc cagcgattca gcccgccaca tcgtcagata tttctgcgac tgtgcgatgg
     1621 tcttttcagc cttgcgcaaa tcagcttcga tttttttgat acgacgccag cgaacgtccg
     1681 gacgttcttt atacttcgcg tgaagcagtg ccgaccgcgc acgttcttcc cagtattccg
     1741 cacgttcaaa gagcatcacg gcacgtttca tgccgttttc aatacgctgc gcatcacgac
     1801 gggcgcggcg ttcgctgtga tgccccacaa gaatgggctg accgggcggg atcatcgcgg
     1861 ccagtctttc cacttcatca agtgcctgtg cggattcact ggcccgcttt ccgctgtatc
     1921 cggtaaaccg ctccgcccgt gcttcctgac gttcagcgag cgtgctgtct tcatcctcaa
     1981 tctctccggc aagtgagagg agcacgtctt cccggcccgg tgtccaggct ggagccacaa
     2041 acagcgcctg ttctggtgcc cagcgaaaac cggcatcatg cactttttta tacgtctccg
     2101 ggtcaagacg tgacacggca tacaggcgca gtttgttgtc atccggtgag taggtcgcgc
     2161 ggtacatctg caccgggctg gcggcttccg gggcggtcat ggtgattgct gctaatgtca
     2221 taaaatgttc tccttccgtc tgttgccgcc cctcgcgggg cggctcacct catcagttaa
     2281 aagcgcgttt cagttcgagt gtgcggagca cctgcccgaa ggccgtgttt ttcatggcgg
     2341 ggatgtgtac accacgcccc cggcgaaaac tccgggcaat gtgtcgcccg aaggctttca
     2401 gcacggggtc aatttccggc gcggggatgt cttcgcgtct gaaacgggga tgacggcggg
     2461 gctttacagc cgctgcggaa ggtgaaacaa tgcttacagt cataaatgga ttccttatga
     2521 caaattggtt aacaatgccg tcaaaattgg tcgcacaatt cttacggcgt ggattaagca
     2581 ggtccctgac ctgctttttt tcatatctgc atctgacgct ttccggtgtt ttcctccttc
     2641 tgttcctgcg ttttcattac ctgccagccg gatgactgac agaacacata aatgcgtatt
     2701 gccggagcct gaaataaatc ctgattttta aagaacatca cccgttgagt ttcgggagcc
     2761 gcggttttga cgtttcgcgg gaacctgctg ctgtattccc tgtggtatga acttcacccg
     2821 tttgagaacc ttcgccgcaa ggggcatcac tgccacggcc tgacgcacct gtcctgggca
     2881 gaacgcaaaa ttttttaccg ggttttaggt aaaaaatttt gtgggctgtt catttaccct
     2941 ggacagtcag tgcggcaggt tgtggctgtc tatgcccccg gcgacaggga ctcatacggg
     3001 tgtgtggcac ccggcaggca atgagcaggg acccgctgca acgacgtgaa gcgcggtttg
     3061 cgaaacggcg ttgctcctta catcccggcg tctttttgcc gggatgccgg gagcgcaggc
     3121 cacggcgcga caggggtggc gcgaccgttc cggtcgctgc aaacccgggg ttttgcgtca
     3181 gggatggaag cccgccaggg cggagacagc tccgctggct ccgttcacga cagcccgccc
     3241 cgccaggggg acgcccggaa aaggcataaa caggagagga acggaaagaa gaggaagaag
     3301 aagaacagta accacagaaa cataacatca ggaaaaaaca ttccggacac atcacaaacg
     3361 ggaagggtaa cgggcgacgc gccagcgtgg cccgcttccc ttccggcagg cgccagcctg
     3421 ccaggggttt tcgcgcgggc tgacaggcgg cctccaccgc ctgccctgta tcacctgtgc
     3481 ctgctgccct gaaggatgta gtgaccaccg aggaaaacag cctgctgatt caccacaaaa
     3541 tcacatattt catcaacaat ctgcgcggtg gtgagcggtt tctgccccat ttcacgacgc
     3601 cgcgcattaa tcacggccct gtaattacgc agaatttcgc gggtttcctt gctgatattc
     3661 attacccatt ctccccgtcc agcgcctgac gcattacccg cataatctca cgttcttccc
     3721 tgacaaatgc gtcaccgtcg cccccgttct cgctccctac agcatggagg cgataaattt
     3781 cactaccagc catcaccatc actttttcca gcagcaccgc agggactgtt acaagacggt
     3841 ttacgcgctt ttcctccatc agttcacgaa caacgtcgtg aacaatgctg acatcagcac
     3901 catgctcaaa ggcatcgtcc agccgctgca tgacggtttc cagctcatcg tctgtcagac
     3961 tccagtcctg ggctatgtga cgcacatccc cacgggtcag caccacggca aaaacgcatt
     4021 cactgttcgg cacgtctgta tccagcactt cgcggataat ttctttaaca gtacagtaca
     4081 tgttctcgtc tcctttacag gggcggcatc gccgccccgt ccgttaatca gtccagtgcc
     4141 gcgtaaatcg cattgcattc ggggtgaaat tcgatgtggc tccacaactg cgcatcgcgc
     4201 atccggtaaa ggtgtgtcag tcccgcatcc ccgctgtcgt ggtacagcca caactggcgg
     4261 ttaatcacaa gggaagtcag gatgatacct gcggcatcag cactcacagt gcggtcaaac
     4321 cagttttcac cgttgaccag atgaaaacgg tcgccatccg gcatcatgta accgccaccg
     4381 tccggcaggc ggatgaaatg ccagaagcct ccctgatatt ccggcatcat tttttgcgcc
     4441 agcgcgtaca catacgcctc ggcatacaga aaatcgttgc caaacaggta aggcagaaag
     4501 gagagacggt cacactcttc aacgttcaga gtgacaggtt tcgcatattg catgatgttt
     4561 tcctccgttg ttcacatatt ccgcgacacc gggcggtgcc gtgcctccat ctttgccggg
     4621 cctgcagggt tgcaagggcg caagggaagc cgcagcctga cgcacacggc tggcgggagt
     4681 tggagggtga gaccgcgaaa tctgagcggg tgaacgaccg gaaaatcacg gcagacggga
     4741 aggagggacc gggtgaccgc agcggttcac cttaccttgc aacccgcccg cagaagcccg
     4801 gctacgatgg aggcacggca cagcccgcac acgacgctga ggccacataa ctgaaccccc
     4861 acaaggccat gccccgcatg gccgggtaaa gcggagccgg cggcgacttc ctttgtgctc
     4921 tgtccgggga ccggcaggaa aaaggcgggg attttccccg ccatcatcag cccctgattt
     4981 tcgccagtgc atccgccatg ctgcggattt catcccgtgc ctccagaaca acctcccgca
     5041 gaatatccct gtcaaaccag cgacgggacg gttcatccgg tatacgcagc acatcacaga
     5101 ccagaccact ggccaggata tgctccacac actcacagac caggtacatt tccacagagg
     5161 aaaaacgggc gccacggttc agaatggcat cgagcaccac gggcagattt tcgtcatctt
     5221 catcaagcca gcaggggtaa tcgctttcct tcacgaccag acgaaagcgg cgcgtctgcc
     5281 gggtcagcaa cgtctgaccg gtggtggtca gcgccgcccg gatacgccgc gcatcgtctg
     5341 ccacctggta acagatgctt cgtactgcat tgcgcaccgc atcaagaact gccctgattt
     5401 cttcctccgt cagccattca ccggcatacg ggaaaaacac ctgcgttccg tctgccggaa
     5461 gtaccggcag cgcctgccac tggctgccgg acagtacaag ttccgccacg gttccggtca
     5521 gtttttgcat ggcagacgcc aggaccggcg gcagcacgcc atcatggcgg ctgataagta
     5581 acgtcgccag ccgggataca tccagtcgca cataaatgct gatccagccc ggatagtgcg
     5641 gattcagttg ttcaatatcc gtctcttccg gccagccctg cgccagcagc aggttacggg
     5701 cgtggtgaca aaccagtgca ttcagcgttt tgttcatgat taatggtctc cctgaatcgc
     5761 cgcccgaagg cggcgataac atcagtcgtt ttcgtgtgtg ttgtttttca gcaggatgcg
     5821 ggcctgatta accaccgcct gacggcgggt gacgcattcc gggacggcga ggccgtaatc
     5881 acgggcggcc cattcacgtt tccacagtgc ctgttcggcg gtctgaatca gggacgccag
     5941 cagttcagcc gggacggtca cctggcggtt ttcgctcacg ttgctgataa tctccatcgc
     6001 cacgccggaa gaaataccgg actcagtccg ctggtcttcc gggatgtcct ccaggcgcgc
     6061 caggacggtc ctgatttcat gatcggacag ggaaatatcc attccgtcag cgagggcctg
     6121 aatctcttcc ggggaccaga taaccagcat caacggcgtg tctcccggat actttgctgc
     6181 cagctcccgg catagcgttt cgcatgttcc gtacattctc atctccttct caatctttga
     6241 agcacgcatc cagttccaga atttcggtat acgcctccat gaacaggcga ttggcgcgat
     6301 acagcgtctg acgtgtcagg cgttcaacaa gcacatcgga atcaaagcga cagcgatgct
     6361 ccatatcgtt cagcctggcc cgaaggtcac acatggtatt ggccacctcc gccacttcgg
     6421 cgcgcagttt ttcgtgtccg gcatagttca tttacgcagc ctccggttca aaccagttat
     6481 cattcgcggc cccctgctgc atggcccgtt gcacggcggc cagtcgttgc tgtccggcac
     6541 ggtgatactg ttcaagcagc tcgataccga tataccggcg tccggactga agggcggcca
     6601 cgcaggttga gccgctgcct gcaaacgggt ccagcacaat cgcgttcggg tgtgtgaagc
     6661 tctcaatcag cggttgcagg ctggtaacgg gcttttccgt cgggtgatga cgattgcctg
     6721 aatatttcca gcccagcacg tcgggcaatg gcttctgagg cagcgccgga cgtccttttg
     6781 ccaggatgta agcgcattcg tgacggtacg cgacatacgc agatttggat gtgtagtttt
     6841 tggtgaagac caggtggcca accacgctga atccggcgcg tttccaggct gccataaagc
     6901 ggtcaatgcg gttccagcca tagaagctga ccattaacgc gttttttttc agcacgcggt
     6961 acatctcatt gctggcaggt tgcagccagt catcgttaac gtcaccggca atggtgcgtc
     7021 cggaacggtc acggaagccc actaggtaag gcgggtcggt taatatgaaa tcaatggcgt
     7081 tatcagggat acgcgccata acatcgatgc agttaccgag gacaaaacgg gacatacaaa
     7141 actccttctc ttacagagga caccgggagg gcacccctgt ggtgcccgtt ccccgctgcc
     7201 tgtgggctgt tcaccgcgtc cggtaacggg tcaggccgac cgtggaatac cgcagccgtc
     7261 gtctttttga cggcaaggct atgccgcgaa agcggcttga agggcgttca tcagaaaagg
     7321 gcgacgggct ggcgtacact gtcaccgacg ccagccggga gcagtaaaag cgcccgtccc
     7381 gtgcccgccg gacgcgggac actgaccaca ggcagcgttc ctgccgcgcc gcgtcagctg
     7441 gcgcagtaaa aagaggagca cggcgacatc ctttgtcgga aaagaaacgg gcggttgccc
     7501 gcccgtctcc gtcatacttc atccgccata atgcgccaca ttgtcgacta tccacgcagg
     7561 tccggtgact tccggcagct catcgtcatc tgtcggggaa gaccattcca gttcgcccca
     7621 caggccactt catgccatac tgttccgtat accagttata tgcagaggga acatctttca
     7681 gaacaccacc gttaaagcca ttcacttcaa catccaggcg ggagggcaga accgtcagca
     7741 gctcaaacgg catgtttttt tctggcagca cgttgtcaca cagacggttc ctccacactg
     7801 aaacatcctc gttactccag aagtcacacc tgtcacctct tttggcggtg aaatgcacgg
     7861 tgatggtttc cctgacatca tccggtaatc cttcccagcg acgctggcca ataccgctct
     7921 gtaaccagag ttcatgcagc atcatgcagt tttattcatc cagtagcaca ccgttctgca
     7981 ggtgcgtcag ccagcgggtg aacgcgatat tctccggcga tacaacgcca cgtccggcag
     8041 ccgtcagggc aacagagtgg cagatgaagc gtgagatgtt gtcacctgca tacacaaccc
     8101 gctggaacga actaccggtc gccagacttt gaaaaaagaa aattcatggt gatttcacta
     8161 tgaaagaatt aaaaaaaaca ctttatattc agtaaaataa gcataaaaaa agcgtggtga
     8221 aaattgcaat aaatatcctc cggcatagcc ctgtctcttg atcagatctc ctgatcaaga
     8281 gacttcatca ccaggtaacc ctcaaccata tcctgaagtc tgaaccagcc atcccacata
     8341 accacccaac cggggcggcc tgtgcgtttg ctgtcatgcc atcgccccag tttagccagt
     8401 ttcagtcagg cccatttcag ggtcagtgcc tgcgccggaa gcggctttcc ttccagctta
     8461 acccacagca gttttcactc tgtcggcgtc agtattttct catagctgtc attttgtgtt
     8521 tcttcactga cgcctccctg ccgcaggccc agcccccgta ccgcgataaa tgccttgata
     8581 accaccatgc gctcaaggtt atcccgggtc tgcattcgca gcgattccac acatgtacca
     8641 ccactttttc acgccttgtg gtattcctct atcagccagc agcccgcgcg tcttcaggtt
     8701 agtggggtat tcccctggtc atcaaatgct gtactgtggc tgaagttaat ggtggtggtg
     8761 tcttccagtg cgaggagcag cggatgtgcc tcacatgccc ttgcagtggc ggtaaatccg
     8821 gcttcggcaa cggcttgcgg ggacacagac gggttacgta tcaggcgata cgcaccttca
     8881 acctgagcag tggactgaga tgatttcaca atagaaagac ctgcatgctg agcgagagaa
     8941 gaggcctgtg acacaaggcg tcgtgtacga cgcggatcac agagacgggt atgtccaaac
     9001 tgctcgttag cctatgaata acaatcagaa agtaccataa cagccccgaa gaaaatgaaa
     9061 tataagagaa gatcaacggg tgaagaaaaa gttcaaaaaa tcgctgccga ggaggaagga
     9121 aactaccgga ttgaaagagt cccccctaaa gcagactgac tgacatcaca aatccccggg
     9181 tggggatttg tgtataagag acagtgccta gctgggggtt ctgtgcacaa aaaggatgcc
     9241 atttggcatc ctgatattaa cccgccatac cctattcagg cagttttctt agaatgtttt
     9301 ttatagcctc atcgatttct aactgcacct cattagatat gcgggagaac tcataaacaa
     9361 caagacgctt tctggggtca gtcttctttc tggcaaattg gttacgatca gaaaactctt
     9421 tcaacttctc agtttcaact gtttttacag gccggtctgt aagcaatttt ccttcagacc
     9481 tgaagaccgc cagaattttg cttttctcaa ttgttggata gtcaggtaaa gccatcagtt
     9541 tttggcgaac tctatccata aactcttcca ctgacatttt tttgttggtt accttttcag
     9601 acagtttcaa caaaaattga tagtccgaca gcgaaatatc attgattacc ggaaatacag
     9661 agatcatttc atccggtact gatgccgcct gaaatgcgcg tgttactttt gccggagaga
     9721 tgctttcagc ctgagcgatc tcttcttttg tcatacttac cccataagta acttcaaggc
     9781 gttttcctag ttctcggagg ctatgctctc tggctgtctg tatatctttt gcaagttgtc
     9841 tcgcgtcggc aatatcaata ctgtcttttg taaccagtat ttcgaatttt acgttgttat
     9901 agatacaggc agcgcgacga cgcgtaccgt ctaatacttc aattcgtcca tctacttccc
     9961 ggccaatagc cggaaaaaat tgctgaagtt taatcgtgcg agttatgtca ctaactgact
    10021 cacgcgaaag catagtctga tcacgtccat taacagatgc atcaacgaac gttttagatt
    10081 ctatatctcc gcttaataca accgttttaa tgaattttgc ttgtttccca gacttaagag
    10141 taaacgtttt gatctctccg tcaccttcaa gcatgcgagc gaactctgaa ttgctattgc
    10201 cgagaactcg ccctcgagct aaaacctttt tcatttggat gctccccgaa tgtattcaat
    10261 gcggtcaaaa acggctttag taaatcgttc cgcttcggta cgagcttttt tcaatgcctc
    10321 tgcacttcct ggatacgaaa cagggttagc actgatgata gtgtcaaaag attcaccaca
    10381 tcgttcgaac ccgtccaatc tcggaagcga tgagtcaaga atattactcg catacacttc
    10441 acgagccagg ctatgggaag tttcgtgatc gcgcttactg gtcattttgg acataaaccc
    10501 tatgctcgca ctcaggcggg gctcaacgcc ttcttcttcc aaacgttcta gcatttcagg
    10561 taaacgagtc aaatatttga gagttgaatg gaaatcaact tgcgccgggg gagtaggggt
    10621 gagaagtaaa tcactcgcag ccaggccatt gagaagaaat ggatcaagat gagggccagt
    10681 atcaataaag acaaaatcat aatcatcagc gatacggtca atgatggttt tcctgagtac
    10741 ctcagaaggc ttaaaaccag gaaggtgttc agcaaccaaa gactcccatt ggcttgctac
    10801 gaagccgtca tcaatagaag cagggatgac atcaacgcca ggaatcatag tgggtcggat
    10861 aaccgcttct cgcagtgttt cagcatcgag attatttagc attgcctggg cggcagtttc
    10921 aaggacagta ccaatactat gggtatggtc caaaaacata gtgctggacg cctgcgggtc
    10981 aaggtcgatc acaaggatac gaaggtcatg acgcagcaaa tccggatgta ctcgtagtgc
    11041 atgcgccagc gttactgtgc tgacggtttt tgaaacccca cctttcaagt tgacgacaaa
    11101 aattacgtaa ggttctttat gaacatcgcg atattttggt atctgtcggt gagcgtaaat
    11161 atcaataacg ttctgaattg ttaatgcata ttgctcgacg ttgcctaccc gctttttatt
    11221 aaattcatag ccactttctt ccatttcttt aacggccagt tctacgatgc gtcggctcag
    11281 cttcggcaat tttgcgaccg cattacgagt atatgtttga tgatactctg tcagattgaa
    11341 ttcttttctt tgctcctcaa tatcccgact catggcctga agtaaagcac ttgctctgaa
    11401 cgctatggta ccgacactac cgtaatctcg tttcatagtt ttcctctgag atagattccc
    11461 tgcccatata ttgcgtacaa agttcaatca cactgtacac aaacatgaaa aacgtacaat
    11521 atattttgca ttatcattaa attgttgtac attgcaagtc accattcaca tcaaaccgca
    11581 tagttagaca ttgtctctga gcaggcgagg ggttagacaa gaccgttggg cgcgttagag
    11641 tatcatatta ggatacgggt gggtgacgcc cacctctggc atagaacgga cattcattga
    11701 tgccatgcca gaatggacgt tcaggttatt ccgtccagtt ctgctggcaa cgcgagatct
    11761 cccctggtat agtgatgcca cagcaaagcg ctcaaacagg gataatatga tggaaatcaa
    11821 ggctcaacag tttgtcacat caacggggcg gcaagtcctt actgacaacg gacaacaagg
    11881 tatggcggcg tggcgggtat cggttccacg actgaaaagc atcaggggcg cgtggcggaa
    11941 gcgatttttg cgaactgcgc ggaactggat aacgaccagc ttaacgagat catcgagtgg
    12001 gttcggctct atcagcgctg aatgccacta tcaggctgcg caagcggcct tttttacgcc
    12061 ccttgtttaa ttcccgcact acctggacgt tcaggtgatt ctgtccatct gtacaaaaaa
    12121 caataaaaga cttgttaaca ggtcatgtaa ggagtatctt tgagactggt taaacagtct
    12181 tgaaaggtgg cctatgccta acattattct cagtgataca agcgccagtg tcagcgagct
    12241 gaagaaaaat ccgatggcga cagtcagcgc cggtgatggt ttcccggtcg ctatcctgaa
    12301 ccgtaatcag cctgctttct actgtgtacc cgcagagctg tacgaaaaga tgctcgatgc
    12361 cctggacgat caggagctgg ttaaactggt agccgaacgc agcaaccaac cgctgcatga
    12421 tgtagacctg gataaatacc tatgaggtat caggtaaaat tcagggaaga tgcgctgaaa
    12481 gagtggcaaa aaatggacaa gactattcag caacagtttg cgaaaaagct aaaaaagtgc
    12541 tgcgacaatc cgcatattcc ttccgcaaaa ctgcgcggga taaaggactg ctacaaaata
    12601 aaattacgtg cgtcaggttt tcgcctggtc tatcaggtga ttgacgaaca attaattatc
    12661 gctgttgtag ctgtgggtaa acgtgagcgc agtgacgttt ataatcttgc cagtgaaaga
    12721 atgagataat tcacggcgga ttatcggttc agccctctca ttaaccgctg atgctgagtt
    12781 ggtgtgtagg gctttgcgta atgcactgga aacacgaccg cgtgatggtc gcctgctgtt
    12841 tcattcagat caaagtgttc agtataaaag caataaatac agaaagttac tctggcgtta
    12901 tggggtaatg caaagtatga gtcgcagagg gaattgtctg gataattcgc cgatggaaag
    12961 agtctttcgc agccttaaaa gtgaatggct tccgaaaggt ggttatggtg attgccatgc
    13021 agtacgcgaa taaatcagtg gataaatgct tattacaacg tttatcgacc tcatacaaac
    13081 aatggtggtt tatcgccttg ccgcatgaag aaaaatggaa acaggttatt ccggtgtcct
    13141 gattttatga tccactacac attcctgacg aacgccactg cataaatcac attgcacatc
    13201 ctcagagagt acgcagtata ggaggggtat atatacatta tcggtatatc aggcgacttc
    13261 tgtctgtatg ttgtcggtgc tcgccgcaga ccggtgaccg ggcgaagccc gcgaaccggc
    13321 gaggaagcgc aatatatctg tagttacagt gagcaggtac gccctgaagc ccttataagc
    13381 ggttgtgtat gctgtttccc tgggatgatt tatccacata tccggaggct cgatccagta
    13441 aacagatcca tgaatgatca acaaaggatc cattaaagat ccccatatcg ctgcaaacct
    13501 tgtcactcat gggccgggac cacgttcaca taagctgtgg catgttatgg ataaactgta
    13561 acaggttcat gataagctgt attcagtaat gcatatactg aagtaagtta atgacataaa
    13621 ctatggtcag tacgccagac tcagttgtta aatacagact gcaggttttt cttcagtcag
    13681 ttgacggggt tctgacacac gattttctgt ttattctttt actgtccaca ggcaggaggc
    13741 tttctggaaa acgaaaattc agacatcaaa aaactgttcg gcgaggtgga taagtcgtcc
    13801 ggtgagctgg tgacactgac accaaacaat aacaacaccg tacaacctgt ggcgctgatg
    13861 cgtctgggcg tctttgtacc tacccttaag tcactgaaga acagtaaaaa aaatacactg
    13921 tcacgcaccg atgccacgga agaactgacg cgtctttctc tggcccgtgc agaaggattc
    13981 gataaggttg agatcaccgg cccccggctg gatatggata acgatttcaa gacctgggtg
    14041 gggatcattc attcctttgc ccgccataac gtgattggtg acaaagttga actgcctttt
    14101 gtcgagtttg caaaactgtg tggtataccc tcaagccagt catcacgcag gctgcgtgag
    14161 cgcatcagcc cttccctgaa acgcattgcc ggtaccgtga tctccttttc ccgcaccgat
    14221 gagaagcaca cccgggaata catcacccat ctggtacagt cagcctacta cgatactgaa
    14281 cgggatattg ttcagttaca ggctgatccc cgcctttttg aactgtacca gtttgacaga
    14341 aaggtccttc ttcagcttaa ggcgattaat gccctgaagc gacgggagtc cgcccaggca
    14401 ctttacacct ttatagagag cctgccccgg gatccggcac cgatatcgct ggcgcggctg
    14461 cgtgcacgcc tcaatctgaa gtctcctgta ttttcccaga accagacggt cagacgggca
    14521 atggagcagt tgcgcgagat tggatatctt gattacacgg agatccagcg ggggcggaca
    14581 aaattcttct gtattcacta ccggcgtccc cggttaaaag cgccgaatga tgagagtaag
    14641 gaaaatcagt tgccaccttc acctgcggaa aaaatcagtc cggagatggc ggagaagctt
    14701 gccctgcttg aaaaactggg catcacgctg gatgacctgg aaaaactctt caaatcccgc
    14761 tgaacataaa ctgtagtcag ataagagaga ctctttactg actacagttt atattaacag
    14821 gtgcagtgag tgatgtgctc actgtagttt atattcagtt tcctgctgca ctgtctgtgg
    14881 ctgagctgcc atctgcctgt tccttacata aaacacggct tactcactac agtttatcta
    14941 cctgctatag cttatgttat gcctgttctg cttacctccg cttatataag gaagagcgta
    15001 tgctgtcact tcaggggagg cagtgtacgc aggatctctg cagcatcgcg cccgtcacct
    15061 gtgaaaggca ctgccagcgt ggcagccata tccagcgcaa aaactctggt ataaacctcc
    15121 atcgaacgtg gatccctgtg accagccagt gcctggatga ctttccgggg ctggcggtga
    15181 tagagcatgt gcatgatata gctgtgccgg aaggtatgtg gtgtgaccgg aatcgaaaaa
    15241 tgcacgccgt cagcttcagc ccgcttcacc gcctgtttca gccagttgcg cattgtctca
    15301 tccgtgaccg gccataacgg ctcccgccgg cggggccggg tggtgatcat ccagctttcc
    15361 atctgcctga catagcttat atctgtcagc ggaaccaggc gcacttcatc ttttggcggg
    15421 cgtccgcgtc gcgcacgcac tttttcggac aggatccgca caaacggtct tactccatcc
    15481 aggtcaaatg attccggcgt cagcatccgg gcttcgccaa tacgcattcc ggtattccag
    15541 agggtggcga acagcatatg gtgacgctga tccggcatat agaaaagaag ggcactcact
    15601 tccggggcca gcaggtaggc cggtgtggcc cccgtggaag tggccattct tctcagggag
    15661 agcgctgttg caaaatccac ccccggcgca atgggtaaca gggagacgcg ttctggtgaa
    15721 ttctgcaggg gaatgacatt gttcatgagt tatttttcac gtagcacacg cactgtttat
    15781 ggatacaggt attctgacct gaacgcccgg atccttccac gacaataaat cgcagcgcgc
    15841 ggatcctttt gtttgattat cacgcattat tgcgggtaaa accaaatgcg taaaggcgca
    15901 ttttgccgca gggatcccgt cttaagccag tcatggtgcg ggctgagagc tggtttaccg
    15961 tttttgggag aattggggta atgccaggaa caggcagata cggagaaaaa tcctgcagat
    16021 aaccttctac gacaactgaa ggtgtctgac gccatattct tagctacttc tcagttttaa
    16081 ggcggcttag tcacaacata aggttatgca taataagcat attttattat gttatgttat
    16141 ggtcttgttc atgcatgaac tgttatctat tcagtcactt ttaacgtttt atatcatact
    16201 gcatatgaca caatcatctt ctatattcag attaaaaata ttatgacgac gatcaaataa
    16261 atttagtgaa atcattactt acaataatat gtttatgaca taatttatca aaaatatccg
    16321 acaaccttcc tgaaaggtta ctgcgaggag tcaaacaatg aagtgtaata acttattcac
    16381 tcttttttat gctctcttta tagcaaaacc aggaagttaa aatgaatcag aagtggaaga
    16441 cgctaatagt ttcagtatta acaccatcaa gtattatatc agggattgcg ttgatagttc
    16501 tctggggata tttcagtcgt ctggatcgac ttgatatttt ctttgatgtg atgaatatta
    16561 aaagcatctt ggtattggtt tgttgtacaa ctattctatc attgattatg atcattttta
    16621 ttttttttat aacatctttt tttataccaa tagttattcc ttatgatata aaaaatcttc
    16681 ctgcttacaa taagattcag ggtaattttt tatctgtaat gatgttatct ggcctcttcc
    16741 ccatggcctt tatatacgtg ttatactgtg catttgattt tgaccagagt gttaaagata
    16801 actctggctg gttatctatt ttaagtatag gagtactaat agttgttatt tcagccataa
    16861 taaacacgag gtatcttgaa tatgacttat cgtttaaaaa taatagaatg aaactgttga
    16921 gacgtgttca gatctatctt atcattccgt tgtctattgc tttactggtg catcttcagg
    16981 taattccact ggagatggtt tttaggaata ttgaaacttc agataagagt attaatttca
    17041 aagtgatagc tgaactggct ttcatgtcat attttatttt tattctgaca atacttcccg
    17101 gcctcattta tctaaaaatg aatccacagc ataaactttc taaaagaatt agttctgcat
    17161 ttgtcgcatc cttgatgata ttattagtta tatcgactca aataaccgta ttaccagtaa
    17221 tttttaccca ttcagttatt aagctctcag gtataagtga cttcaaaata catagctaca
    17281 ttattaaaac aagtgaatat ccggaggagt ttttttccaa tgcagtatgg ggtaaaaaaa
    17341 atataaaacc gggggaatat tactctgtgc aggctgtttc tatgtttacg acaaaccagt
    17401 ttattctcct ttgtcccaag gatattataa ggttttatcg ggaaagctgg aagttcgacc
    17461 tgcttaatgt agacttcgat acaaatacca ggaagaaact ccaggaagaa gcagcatatt
    17521 gcgtaccaat ttctgcaata tctgtaaaac gatgggatat gcctcttcag ggctcgaaac
    17581 cctcaaatta gaaggccgct cgtgcgactg tattagatag taatgcggta tggaaaaata
    17641 gtgatggcaa gatgaacttg acttggctaa tcaaataccg gcgtagtgat taggaaacag
    17701 ggctcggata acattattgc gcggttcatt gaacctactg atgacgccgg atatatagtc
    17761 gtaaaccgtt tcgaaccagt acttatatca ccaggagagt ccatatatga ccaccgttta
    17821 cagtaaaaac ggtatctgtt ttctgatccc tccagcgtca aactaccgtt gtaatggtga
    17881 ggtctggtgc tattgtaata attcagaata taatctctga tttatttccg aacctcgccc
    17941 ttgcctgcgt aaccatccgt cgcccccatc ctgttttcag actgcggaaa aaggcaagtt
    18001 tttatccccc cacagagatt caatcatata tcgcgtgttt tggaagtgac agttcttatt
    18061 cctgagaata ctgcatgtgc aacacactcg gcatccatcg ataccacttt tatctccaat
    18121 aggcaggata aaaaacaaga ctgtgaatta aagtaatcgg caaatgaatg agttagaaga
    18181 tactgaaaat gaatcaagga
//
LOCUS       DAZFFE010000096        18097 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZFFE010000096 DAZFFE010000000
VERSION     DAZFFE010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.096"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            152..910
                     /gene="ydiY"
                     /locus_tag="ACHJ69_003608"
     CDS             152..910
                     /gene="ydiY"
                     /locus_tag="ACHJ69_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO5016507.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(963..1070)
                     /gene="yniD"
                     /locus_tag="ACHJ69_003609"
     CDS             complement(963..1070)
                     /gene="yniD"
                     /locus_tag="ACHJ69_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO5016508.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1241..2270)
                     /locus_tag="ACHJ69_003610"
                     /pseudo
     CDS             complement(1241..2270)
                     /locus_tag="ACHJ69_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            2793..4721
                     /gene="thrS"
                     /locus_tag="ACHJ69_003611"
     CDS             2793..4721
                     /gene="thrS"
                     /locus_tag="ACHJ69_003611"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO5016509.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4725..5267
                     /gene="infC"
                     /locus_tag="ACHJ69_003612"
     CDS             4725..5267
                     /gene="infC"
                     /locus_tag="ACHJ69_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO5016510.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            5364..5561
                     /gene="rpmI"
                     /locus_tag="ACHJ69_003613"
     CDS             5364..5561
                     /gene="rpmI"
                     /locus_tag="ACHJ69_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO5016511.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5614..5970
                     /gene="rplT"
                     /locus_tag="ACHJ69_003614"
     CDS             5614..5970
                     /gene="rplT"
                     /locus_tag="ACHJ69_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO5016512.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    6087..6211
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6093..6137
                     /gene="pheM"
                     /locus_tag="ACHJ69_003615"
     CDS             6093..6137
                     /gene="pheM"
                     /locus_tag="ACHJ69_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO5016513.1"
                     /translation="MNAAIFRFFFYFST"
     gene            6421..7404
                     /gene="pheS"
                     /locus_tag="ACHJ69_003616"
     CDS             6421..7404
                     /gene="pheS"
                     /locus_tag="ACHJ69_003616"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO5016514.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            7419..9806
                     /gene="pheT"
                     /locus_tag="ACHJ69_003617"
     CDS             7419..9806
                     /gene="pheT"
                     /locus_tag="ACHJ69_003617"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO5016515.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPIAVEAPDACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLDADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQVREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9811..10110
                     /gene="ihfA"
                     /locus_tag="ACHJ69_003618"
     CDS             9811..10110
                     /gene="ihfA"
                     /locus_tag="ACHJ69_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO5016516.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            10211..11191
                     /gene="btuC"
                     /locus_tag="ACHJ69_003619"
     CDS             10211..11191
                     /gene="btuC"
                     /locus_tag="ACHJ69_003619"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO5016517.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGELWILPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            11253..11804
                     /gene="btuE"
                     /locus_tag="ACHJ69_003620"
     CDS             11253..11804
                     /gene="btuE"
                     /locus_tag="ACHJ69_003620"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO5016518.1"
                     /translation="MQDSILTTVVKDIDGKVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            11804..12553
                     /gene="btuD"
                     /locus_tag="ACHJ69_003621"
     CDS             11804..12553
                     /gene="btuD"
                     /locus_tag="ACHJ69_003621"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO5016519.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPANLAQAYGMNFRRLDIEGHRMLISTI"
     gene            12631..13095
                     /gene="nlpC"
                     /locus_tag="ACHJ69_003622"
     CDS             12631..13095
                     /gene="nlpC"
                     /locus_tag="ACHJ69_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO5016520.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            13342..14055
                     /gene="ydiV"
                     /locus_tag="ACHJ69_003623"
     CDS             13342..14055
                     /gene="ydiV"
                     /locus_tag="ACHJ69_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO5016521.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNFAG
                     ELLKYPFIELLINENYPHFNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQIIPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            14118..15554
                     /gene="selO"
                     /locus_tag="ACHJ69_003624"
     CDS             14118..15554
                     /gene="selO"
                     /locus_tag="ACHJ69_003624"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO5016522.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVESGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLADEEDKYRLW
                     FTDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDGLNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEITDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(15558..15749)
                     /gene="hemP"
                     /locus_tag="ACHJ69_003625"
     CDS             complement(15558..15749)
                     /gene="hemP"
                     /locus_tag="ACHJ69_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO5016523.1"
                     /translation="MRYTDSRKLTPETDASHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(15881..16927)
                     /gene="aroH"
                     /locus_tag="ACHJ69_003626"
     CDS             complement(15881..16927)
                     /gene="aroH"
                     /locus_tag="ACHJ69_003626"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO5016524.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQPRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHAEDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(17084..17917)
                     /gene="ppsR"
                     /locus_tag="ACHJ69_003627"
     CDS             complement(17084..17917)
                     /gene="ppsR"
                     /locus_tag="ACHJ69_003627"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO5016525.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         4586 a         4157 c         4728 g         4626 t
ORIGIN      
        1 atgcttattc aaaaaatgaa ataagaatac attgctaagt tattcgcgcc tttttataat
       61 tcgttacctt tccagggacg ctaatcttta tccttaagaa agtttccggg accgcgatgg
      121 tctctttttt tgttgtacgg actcttgata aatgaagctt ttgaagacag ttcctgccat
      181 cgttatgttg gcggggggca tgtttgcgtc actgaatgca gctgccgatg attccgtttt
      241 tactgtcatg gacgatcctg ccagcgctaa aaaacctttc gaaggtaatc tgaacgcagg
      301 atatcttgca caatcaggca atacgaaaag ctcctcactt acggctgaca ccaccatgac
      361 ctggtatgga caaaccactg cctggtcgct gtgggggaac gccagtaata cttcttctaa
      421 tgatgagcgt tcttcagaaa aatatgcggc gggcgggcgt agccgtttta atttaactga
      481 ttatgactat ttatttgggc aggcaagctg gctgacagac cgttataacg gctatcgaga
      541 gcgcgatgtg ttgaccgcgg gttatggtcg ccagtttttg aatggcccag tacacagttt
      601 ccgctttgaa ttcggcccag gtgtgcgtta cgacaaatat accgacaatg ccagtgaaac
      661 ccagccgctg ggctatgcct ccggggctta tgcatggcag ttgactgaca acgctaaatt
      721 tacgcagggt gtttcggtct tcggcgcgga agacacaacg ctgaactccg aaagcgcatt
      781 aaatgttgct attaatgaac attttggact gaaggtggct tacaacgtca catggaactc
      841 tgaacctcca gagtctgcac cggaacatac cgatcgtcgg acaaccttat cgctgggcta
      901 cagcatgtaa aattatctgc cggaccgtgt tatgcggtct ggctgttttc gtttgtcact
      961 gctcagcccc aaatcattgc tgcccaacgt agcaacaaca tagcgccaca gattgccagt
     1021 agcaccacca ccatcttcca gtgtttttta tcaaagcgtt tagtcggcat accagtgtcc
     1081 tgcatatctc ttgtgcgtga taacggagtg taacaaaagg gacaggcatc aagggatttt
     1141 ttgttattac agtcgtcggg tggcgttgta cacctggtca ctgtttctgc attttatgaa
     1201 aaaaattatg tatcgatata caaatatcat aaatagcggg ttataaatac gtctttagtt
     1261 catcagcact aaaactcagg ctttggctga ttacatttat agcagcataa tatgtttcta
     1321 cagttttatg atgattatga tgaatggcta tatgaacagc tgacatgttg tcatgatttt
     1381 tagcggccaa cagtgtaaat aactgacgtt ggctaaaaga atattttttt caaaagtacc
     1441 caacgttgat atataagata acacgacatc tttacttact gacaaagtat gattgtctat
     1501 atataaaatc gcggacatat gactgacatt gtttgccatc attcgacaga agaaaatatc
     1561 taaagcaaag ccaaaatcct gactcgcgat aacgcgactc cttgcaggag gatggcgaag
     1621 taatgctctg aactccgcgt cccggactaa ggagacatgc tgctgtatgg attcaacaga
     1681 ggagtaatgt ttaatcaggt tgactttgcc ttttccattt ctcaatgaat caagggcgta
     1741 ttgtaatgac ggatggcgac aaaaaatagt gtcagaagga gattgcaaaa aaatgcacta
     1801 tcattgagat ttactgttgg ctgtatggtt ttttctctat gaattttttt ggacagtttc
     1861 atgcaatggt tacgtttttc ggcagttaac tgttcctgtt caatataatc cagtatgctt
     1921 cgcaaatggt tagctttaat aggcgtgatt tccagaaata aggaaatgat ttctttttta
     1981 tagtgtaatg taatgagtgt attttctgca tggatgggtg aagtggcaaa gttaatgata
     2041 aaaagggcag tggaataaca attttgtgaa atttttcgac agattgcggt aaattcctga
     2101 gtaacacagt atgacagcat cgaacttaaa taagaggtat caagtagggt gatgagctgg
     2161 cgcgcaaatt gattaatagg ctctgatgaa ggcgttagta tgtcgctatc gatgtgcatt
     2221 ctaagaggag actcataatg agttctgata atgatatcgt tttcactcat aatctattcg
     2281 ccttatatat gtctggagat tttgaggatg atgattaatt attatcgcca gaacgaaatg
     2341 cgcacgaaaa attgctataa gaattgtgtc acttttatca ttgtgatatg actacttcgt
     2401 cattaatctc atgatcgttt aaatactacc gccaaatcac gcaacgaact ttgctggatt
     2461 cagtttatgc tgctgtaaat ccgctcgtag taaacctttc agacactctg tgatattatc
     2521 agtggttctt taagcgtttt gctggtgtac tcactacaaa cgaattgcga atcgaatcaa
     2581 tgtgaaacgg aaaggtacaa tctccctttt tttagtgtta acatcgttca accgggtgga
     2641 aggtgagagg ttaatgtctt tcatttgtgt acctgattgc gaaccaattt agcatttgtt
     2701 ggctagatgg tttcgcaatg aactgttaat aaacaaattt ttctttgtat gtgatctttc
     2761 gtgtgggtca ccactgcaaa taaggatata aaatgcctgt tataactctt cctgatggca
     2821 gccaacgcca ttacgaccac gctgtaagcc ccatggatgt tgcgctggac attggtccag
     2881 gtctggcgaa agcctgtatt gcagggcgcg ttaatggcga actggttgat gcttgcgatc
     2941 tgattgaaaa cgacgcacaa ctgtcgatca ttactgccaa agacgaagaa ggtctggaga
     3001 tcattcgtca ctcctgtgcg cacctgttag ggcacgcgat taaacaactt tggccgcata
     3061 ccaaaatggc aatcggcccg gttattgaca acggttttta ttacgacgtt gatcttgacc
     3121 gcacgttaac ccaggaagat gtcgaagcac tcgagaagcg gatgcatgag cttgctgaga
     3181 aaaactacga cgtcattaag aagaaagtca gctggcatga agcgcgtgaa actttcgcca
     3241 accgtgggga gagctacaaa gtctccattc ttgacgaaaa catcgcccat gatgacaagc
     3301 caggtctgta cttccatgaa gaatatgtcg atatgtgccg cggtccgcac gtaccgaaca
     3361 tgcgtttctg ccatcatttc aaactaatga aaacggcagg ggcttactgg cgtggcgaca
     3421 gcaataacaa aatgttgcaa cgtatttacg gtacggcgtg ggcagacaaa aaagcgctta
     3481 acgcttacct gcaacgcctg gaagaagccg cgaaacgcga ccaccgtaaa atcggtaaac
     3541 agctcgacct gtaccatatg caggaagaag cgccgggtat ggtattctgg cacaacgacg
     3601 gctggaccat cttccgtgaa ctggaagtgt ttgttcgttc taaactgaaa gagtaccagt
     3661 atcaggaagt taaaggtccg ttcatgatgg accgtgtcct gtgggaaaaa accggtcact
     3721 gggacaacta caaagatgca atgttcacca cgtcttctga gaaccgtgaa tactgcatta
     3781 agccgatgaa ctgcccgggg cacgtacaaa ttttcaacca ggggctgaag tcttatcgcg
     3841 atctgccgct gcgtatggcc gagtttggta gctgccaccg taacgagccg tcaggttcgc
     3901 tgcatggcct gatgcgcgtg cgtggtttta cccaggatga cgcgcatatc ttctgtactg
     3961 aagaacaaat tcgcgatgaa gttaacggat gtatccgttt agtctatgat atgtacagca
     4021 cttttggctt cgagaagatc gtcgtcaaac tctccactcg tcctgaaaaa cgtattggca
     4081 gcgacgaaat gtgggatcgt gctgaggcgg acctggcggt tgcgctggaa gaaaacaaca
     4141 tcccgtttga atatcaactg ggtgaaggcg ctttctacgg tccgaaaatt gaatttaccc
     4201 tgtatgactg cctcgatcgt gcatggcagt gcggtacagt acagctggac ttctccttgc
     4261 cgtctcgtct gagcgcttcc tatgtgggcg aagacaacga gcgtaaggta ccggtaatga
     4321 ttcaccgcgc aattctgggg tcgatggaac gttttatcgg tatcctgacc gaagaatttg
     4381 ctggtttctt cccgacctgg cttgcgccgg ttcaggttgt tatcatgaat attaccgatt
     4441 cacagtctga ttacgttaac gaattgacgc aaaaactatc aaatgcgggc attcgtgtta
     4501 aagcagactt gagaaatgag aagattggct ttaaaatccg cgagcacact ttgcgtcgcg
     4561 tcccatatat gctggtctgt ggtgataaag aggtggaatc aggcaaagtt gccgttcgca
     4621 cccgccgtgg taaagacctg ggaagcatgg acgtaaatga agtgatcgag aagctgcaac
     4681 aagagattcg cagccgcagt cttaaacaat tggaggaata aggtattaaa ggcggaaaac
     4741 gagttcaaac ggcgcgccct aaccgtatca atggcgaaat tcgcgcccag gaagttcgct
     4801 taacaggtct ggaaggcgag cagcttggta ttgtgagtct gagagaagct ctggagaaag
     4861 cagaagaagc cggagtagac ttagtcgaga tcagccctaa cgccgagccg ccggtttgtc
     4921 gtataatgga ttacggcaaa ttcctctatg aaaagagcaa gtcttctaag gaacagaaga
     4981 aaaagcaaaa agttatccag gttaaggaaa ttaaattccg tcctggtaca gatgaaggcg
     5041 actatcaggt aaaactccgc agcctgattc gctttctcga agagggtgat aaagccaaaa
     5101 tcacgctgcg tttccgcggt cgtgagatgg cgcaccagca aatcggtatg gaagtgctta
     5161 atcgcgtgaa agacgatttg caagaactgg cagtggtcga atccttccca acgaagatcg
     5221 aaggccgcca gatgatcatg gtgctcgctc ctaagaagaa acagtaaggc cttcaagtag
     5281 cactgtctgt ggagcctttg ggcttcacag acattgttcg cctttgtttc gtttattaac
     5341 aatgcgaagt ggaagttatt aaaatgccaa aaattaagac cgtacgcggt gctgctaagc
     5401 gcttcaaaaa aaccggtaaa ggtggtttta agcacaagca cgctaacctg cgtcacattc
     5461 tgaccaaaaa agcgaccaaa cgtaaacgtc acctgcgtcc gaaagccatg gtttccaaag
     5521 gcgatctggg cctggtaatc gcgtgcctgc cgtacgcata agccgttaac gtttttaact
     5581 ttttaattag aatatagata caggagagca catatggctc gcgtaaaacg tggtgttatt
     5641 gcacgcgcac gtcacaagaa aattttgaaa caagctaaag gctactacgg tgcgcgttct
     5701 cgcgtatacc gcgttgcctt ccaggctgtt atcaaagctg gtcagtatgc ttaccgcgac
     5761 cgtcgtcaac gtaagcgtca gttccgtcaa ctgtggattg cgcgtatcaa cgcagcagca
     5821 cgtcagaacg gtatttctta cagcaaattc atcaatggcc tgaaaaaagc ctctgttgaa
     5881 atcgaccgta agatcctggc tgatatcgca gtattcgaca aagtagcgtt caccgctctg
     5941 gttgaaaaag cgaaagcagc tctggcataa gccagttgaa agagggagct agtctccctc
     6001 ttttcgtttc aacgccatca aaacattgac ttttatcgcc gtagcctttt caataaaggt
     6061 cttttgaaga gtaccaaaag gtaacgcaag caatgaatgc tgctattttc cgcttctttt
     6121 tttactttag cacctgactc caggaggcta gcgcgtgaga agagaaacgg aaaacagcgc
     6181 ctgaaagcct cccagtggag gctttttttg tatgcgcgtt ttgaaaaata aaaagcctgt
     6241 ctcatcaggc gtaattgtcg cagccagtct ggacacggcc agcgcggaga aaccggagcg
     6301 tacttaagta cgtgaggatt tcgagcactg cccgggacca aaatggcaag taaaatagcc
     6361 tgatgggatg ggctctaagt ccaacgaacc agtgtcacca ctgacacaat gaggaaaaac
     6421 atgtcacatc tcgcagaact ggttgccagt gcgaaggcgg ccattagcca ggcgtcagat
     6481 gttgccgcgt tagataatgt gcgcgtcgaa tatttgggta aaaaagggca cttaaccctt
     6541 cagatgacga ccctgcgtga gctgccgcca gaagagcgtc cggcagctgg tgcggttatc
     6601 aacgaagcga aagagcaggt tcagcaggcg ctgaatgcgc gtaaagcgga actggaaagc
     6661 gccgcactga atgcgcgtct ggcggcggaa acgattgatg tctccctgcc gggtcgtcgc
     6721 attgaaaacg gtgggctgca tccggtgacc cgtaccatcg accgtatcga aagtttcttc
     6781 ggtgagcttg gctttaccgt ggcaaccggg ccggaaatcg aagacgatta tcataacttc
     6841 gatgctctga acattcctgg tcaccacccg gcgcgcgctg accacgacac tttctggttt
     6901 gacgctaccc gcctgctgcg tacccagacc tctggcgtac agatccgcac catgaaagcc
     6961 cagcagccac cgattcgtat catcgcgcct ggccgcgttt atcgtaacga ctacgaccag
     7021 actcacacgc cgatgttcca tcagatggaa ggtctgattg ttgataccaa catcagcttt
     7081 accaacctga aaggcacgct gcacgacttc ctgcgtaact tctttgagga agatttgcag
     7141 atccgcttcc gtccttccta cttcccgttt accgaacctt ctgcagaagt ggacgtcatg
     7201 gggaaaaacg gtaaatggct ggaagtactg ggctgcggga tggtgcatcc gaacgtgctg
     7261 cgtaacgttg gcatcgaccc ggaagtttac tctggttttg ccttcgggat ggggatggag
     7321 cgtctgacca tgttgcgtta cggcgtcacc gacctgcgtt cattcttcga aaacgatctg
     7381 cgtttcctca aacagtttaa ataaggcagg aatagattat gaaattcagt gaactgtggt
     7441 tacgcgaatg ggtgaacccg gcgattgata gcgacgcgct ggcaaatcaa atcacgatgg
     7501 cgggcctgga agttgatggt gtagaaccgg ttgccggtag cttccacggc gtggtcgttg
     7561 gtgaagtggt tgagtgtgcg cagcatccga acgctgacaa actgcgtgtg acaaaagtga
     7621 atgtcggcgg cgatcgcctg ctggacatcg tctgcggtgc gccaaactgc cgtcagggcc
     7681 tgcgtgtggc ggtagcgacc attggtgctg ttctgccggg tgatttcaaa attaaagccg
     7741 cgaaactgcg tggcgaaccg tctgaaggga tgctgtgctc cttctctgag ctgggaattt
     7801 ctgacgatca taacggcatt atcgagctgc ctgcggatgc gccgattggc accgacatcc
     7861 gcgaatacct gaaactcgat gacaacacca tcgaaattag cgtgacgcca aaccgtgccg
     7921 actgcttagg tatcattggt gttgcgcgcg acgttgccgt gttgaaccag ctgccgctgg
     7981 ttgaaccgga aatcgttccg gttggtgcga ccatcgacga cacgctgccg attgcagtag
     8041 aagcgccgga tgcctgcccg cgctatcttg gccgtgtggt aaaaggcatt aacgttaaag
     8101 cgccaactcc gctgtggatg aaagaaaaac tgcgtcgttg cgggatccgt tctatcgatg
     8161 cagttgttga cgtcaccaac tatgtgctgc tcgaactggg ccagccaatg cacgctttcg
     8221 ataaagatcg cattgaaggc ggcattgtgg tgcggatggc gaaagagggc gaaacgctgg
     8281 tgttgctcga cggcactgaa gcgaagctgg atgctgacac cctggtcatc gccgaccaca
     8341 acaaggcgct ggcgatgggc ggcatatttg gtggcgaaca ctctggcgtg aatgacgaaa
     8401 cgcaaaacgt tctgctggaa tgcgctttct tcagcccgct gtctatcacc ggtcgtgctc
     8461 gtcgtcatgg cctgcatact gatgcgtctc accgttatga gcgtggcgtt gatccggcac
     8521 tgcagcacaa agcgatggaa cgtgcgaccc gtctgctgat cgacatctgc ggtggtgaag
     8581 cgggtccggt aattgatatc actaacgaag caacgctgcc gaagcgtgca accatcactt
     8641 tacgtcgtag caaactggat cgcctgatcg gccatcatat tgctgatgag caggtaacag
     8701 acattctgcg tcgtctcggc tgcgaagtga ccgaaggcaa agacgagtgg caggcagttg
     8761 cgccgagctg gcgtttcgac atggagattg aagaagatct ggtcgaagaa gtcgcgcgtg
     8821 tttacggcta caacaacatc ccggatgagc cggtacaggc aagcctgatt atggggactc
     8881 accgtgaagc tgacctgtcg ctcaagcgcg taaaaacgct gctcaacgac aaaggctatc
     8941 aggaagtgat cacctacagc ttcgttgacc cgaaagtgca gcagatgatc catccaggcg
     9001 ttgaagcctt actgctgcca agcccgatct ctgttgaaat gtcagcaatg cgtctttccc
     9061 tgtggaccgg cctgctggca accgtggtgt acaaccagaa ccgtcagcag aatcgtgtac
     9121 gcattttcga aagcggtctg cgtttcgtgc cagatactca ggcaccgttg ggtattcgtc
     9181 aggatctgat gttagccggt gtgatttgcg gtaaccgtta cgaagagcac tggaacctgg
     9241 caaaagaaac cgttgatttc tatgatttga aaggcgatct tgaatccgtt ctcgacctga
     9301 ccggtaaact gaatgaggtt gagttccgtg cagaagcgaa tccggcactg catccggggc
     9361 aatccgcagc gatttatctg aaaggtgaac gtattggttt tgttggggtt gttcatcctg
     9421 aactggaacg taaactggat cttaacggtc gcactctggt gttcgaactg gagtggaaca
     9481 agctcgcaga ccgcgtggtg cctcaggtgc gcgagatttc tcgcttcccg gcgaaccgtc
     9541 gtgacatcgc ggtggtggtc gcagaaaacg ttcccgcagc ggatatttta tccgaatgta
     9601 agaaagttgg cgtaaatcag gtagttggcg taaacttatt tgacgtgtac cgcggtaagg
     9661 gtgttgcgga ggggtataag agcctcgcca taagcctgat cctgcaagat accagccgta
     9721 cactcgaaga agaggagatt gccgctaccg tcgccaaatg tgtagaggca ttaaaagagc
     9781 gattccaggc atcattgagg gattgaacct atggcgctta caaaagctga aatgtcagaa
     9841 tatctgtttg ataagcttgg gcttagcaag cgggatgcca aagaactggt tgaactgttt
     9901 ttcgaagaga tccgtcgcgc tctggaaaac ggcgaacagg tgaaactctc tggttttggt
     9961 aacttcgatc tgcgtgataa gaatcaacgc ccgggacgta acccgaaaac gggcgaggat
    10021 attcccatta cagcacggcg cgtggtgacc ttcagacccg ggcagaagtt aaaaagccgg
    10081 gtcgaaaacg cttcgcccaa agacgagtaa tctgatctaa ctaaaaaggc cgctctgcgg
    10141 ccttttttct tttcactgtc gaagagtcac cgtaaaatca acgccatgac acttcagcag
    10201 aacggatacc atgctgacac ttgcccgcca acaacagcga caaaatattc gctggttatt
    10261 atgcttgtca gttttgatgc tactggcgct tctcttaagc ctttgcgccg gtgaactatg
    10321 gattttgcca ggtgactggt tttctcctcg tggcgaactg ttcgtctggc aaattcgcct
    10381 gccacgtacg ctggctgtat tgctggttgg tgcggcgctg gctatatccg gcgctgtaat
    10441 gcaggcgttg tttgaaaatc ctctggcaga acctggacta cttggcgtct ctaacggcgc
    10501 aggcgtgggg cttatcgccg cggtattact tgggcaaggg caactcccca actgggcgct
    10561 agggctgtgt gcgattgctg gcgcgcttat catcacttta atactcttac gtttcgcccg
    10621 tcgtcatctt tcgaccagtc ggttattgct ggctggcgtt gcattaggga ttatctgtag
    10681 cgcactaatg acgtgggcta tctacttttc cacctctgtt gatttacgtc agctgatgta
    10741 ctggatgatg ggcggttttg gcggcgtaga ctggcggcaa agctggctga tgctggcatt
    10801 gatcccggtg ttgttgtgga tctgttgtca gtccaggccg atgaatatgt tagcacttgg
    10861 cgagatctcg gcgcggcaac tgggtttacc cctgtggttc tggcgcaatg tgctggtggc
    10921 agcgaccggc tggatggttg gcgtcagtgt ggcgctggcg ggtgctatcg gctttattgg
    10981 tctggtgatc ccacatattc tccggctgtg tggtttaacc gatcatcgcg tattacttcc
    11041 cggctgcgcg ctggcagggg cgagcgcatt gctgctggcc gatattgtag cgcgcctggc
    11101 attagctgcc gcagagctgc caattggcgt ggtcaccgca acgttgggtg cgccggtgtt
    11161 tatctggtta ttgttaaaag caggacgtta gccgcaaaac acggtctatg attaaaagct
    11221 aagattttta ctgaccatac ccaggagaaa cgatgcaaga ttccattctg acgaccgtag
    11281 tgaaagatat tgacggtaaa gtgaccacgc tggagaagta cgccggtaat gtgctgttga
    11341 ttgtcaatgt cgcctcaaag tgtggcttaa cgccgcaata tgagcagttg gagaatattc
    11401 agaaagcctg ggctgatcgt ggttttgtgg tgctgggatt cccgtgtaac cagtttctgg
    11461 agcaagaacc gggtagcgat gaagagatta aaacttattg caccaccaca tggggggtga
    11521 cgttcccgat gttcagtaag attgaagtta atggcgaagg acgtcatccg ctgtatcaaa
    11581 aattgattgc cgcagcgccg actgcagtcg cgccggaaga gagcggattc tatgcccgaa
    11641 tggtcagcaa aggtcgtgca ccgctgtacc ctgatgatat tttatggaat tttgagaaat
    11701 ttttagttgg cagggacgga ttggtcatcc agcgtttttc cccggatatg acgccggaag
    11761 atcccattgt gatggaaagc attaaactgg cgttggcaaa ataatgtcta ttgtgatgca
    11821 gttacaggat gttgcggaat ctacccgcct ggggccgctt tctggcgagg ttcgggctgg
    11881 ggagatcctg cacctggtgg ggccgaatgg cgcgggtaag agtaccttac tggcgcgaat
    11941 ggccggaatg accagcggta agggaagcat tcagttcgcg gggcaaccac tggaagcatg
    12001 gtctgcaaca aagctcgcgc tgcatcgcgc ctatctttca caacagcaga cgccgccgtt
    12061 tgcaatgccg gtctggcact acctgacact gcatcagcac gataaaacgc gtaccgaact
    12121 actgaatgat gtcgcagggg cgctggctct tgatgacaaa ctcggacgta gcaccaatca
    12181 actttccggc ggtgaatggc aacgcgtacg tcttgctgcg gtggtgctgc aaatcacacc
    12241 acaagccaat cccgcaggcc aattgctgct tcttgatgag ccgatgaaca gtcttgatgt
    12301 tgcgcaacaa agtgccttag acaaaattct gagcgcgctg tgtcagcaag gactggcgat
    12361 tgtgatgagc agtcacgatc tcaaccacac attgcgtcat gcgcatcggg cgtggttgct
    12421 aaaaggtgga aaaatgctgg ccagtggacg cagagaagag gtgctcacgc cggcaaatct
    12481 ggcgcaggcc tatgggatga attttcgccg tctggatatc gaaggtcaca gaatgctgat
    12541 ttcgaccatc tgatttcatt agtatcttga aaatcgggta attacgacgc taaattaatg
    12601 caagaataaa aaacagagga accgtacgga atgcgtttct gccttatttt gatcacagca
    12661 ctgtttctgg ccgggtgtag ccaccataaa gcaccgccgc caaatgccag actttctgat
    12721 tcgattaccg ttattgccgg tttgaacgac cagctacaaa gctggcatgg cacgccgtat
    12781 cgttatggtg gcatgacgcg gcgcggtgtg gactgttcgg gatttgtggt tgtgacgatg
    12841 cgcgatcgtt tcgatttgca gctgccccgt gaaaccaaac aacaagcctc tattggcacg
    12901 caaattgata aagacgagtt gctgccaggt gacctggtct ttttcaaaac gggttccgga
    12961 caaaatggtt tgcatgtagg tatttacgat accaacaacc aatttatcca cgcctctacc
    13021 agcaagggag tgatgcgttc ctcacttgat aatgtctatt ggcagaaaaa tttctggcag
    13081 gcgagacgaa tctagtatgt cgaaaaatgg acggcgatga tacatcgccg tccatttact
    13141 cctgaataca gtagtatttt gcagacaaaa attccgtaaa tggattcttc gtttataatc
    13201 aggataatct aaaaaaagga cccctgaaaa agtggttaac atggaacaaa tgaaagttac
    13261 tccttctgta attgttatgt cttatggcct catccctttt agccggatac tgaaaaacat
    13321 ccttcgagag ggacggttac catgaagatt tttttggaga atctttatca ttctgattgc
    13381 tatttcctgc cgataagaga taaccagcaa gaccttgtcg gcgttgagtt aattacccat
    13441 ttctccagtg aagatggtac ggttcgtatc ccgacgagtc gggttatagc gcaacttacc
    13501 gaggagcaac attggcaatt attttctgaa cagttggaat tactaaaatc atgccaacac
    13561 ttttttattc aacataaact ctttgcctgg cttaatttaa cgccacaagt ggctacatta
    13621 ttattagaca gggataattt cgcaggcgaa ctattaaaat atccgtttat tgaactgttg
    13681 attaatgaga attacccgca tttcaatgaa ggaaaagata atcgcgatct tttatcctta
    13741 tcgcaaatgt atccccttgt gttaggtaat ttgggagcag gtaatagcac aatgaaagcg
    13801 gtgtttgatg gtcttttcac ccgcgtcatg ctggataaaa gttttattca gcaacaaatc
    13861 acgcatcgct ccttcgaacc gtttattcgt gcgatccagg cgcaaatttc cccttgttgc
    13921 aattgcatca tcgcgggggg cattgatacg ccagaaatat tggcacagat tatccccttt
    13981 gatttccatg ctctccaggg atgcctgtgg cctgcggttc ctataaatca gataacgacg
    14041 ttggttcagc gataaccctc ctgtttgctg gtgtttaaga cgagagtaac cgtctacact
    14101 atcaaacagg aggatctatg accctgtctt ttattacccg ctggcgcgat gaattgccag
    14161 aaacctatac agcactttcc cctacgcctt taaataatgc ccggctgatt tggcataata
    14221 ccgaactggc taacacgctg agtattccat cgtcgctgtt taaaaatggc gcaggtgtct
    14281 ggggcggcga aaccctactg cctggtatgt caccacttgc gcaggtttac agtggtcatc
    14341 agtttggcgt ctgggcgggc caactgggtg atgggcgcgg cattttactc ggcgaacaac
    14401 tgcttgctga tggcactaca atggactggc atctgaaagg tgctggcctg acgccttatt
    14461 cgcgaatggg tgatggacgg gcggttttac gttcgacgat acgagaaagt ctcgccagtg
    14521 aggcgatgca ttatctgggc attccgacga cccgcgcttt aagtatcgtc accagcgatt
    14581 cgccagtgta tcgggaaacg gtggagtcag gcgcgatgct gatgcgtgtg gcaccaagtc
    14641 atctgcgctt tggtcatttc gaacattttt actatcgccg cgagccggaa aaggttcgtc
    14701 agttagctga ctttgccatt cgtcattact ggtcacatct tgcagatgag gaggacaaat
    14761 accgtctctg gtttaccgat gttgtcgcac gtaccgcctc gttaattgcc caatggcaga
    14821 cggtcggctt tgctcatggg gtgatgaata ccgacaacat gtcgctgctg gggctgacgc
    14881 ttgattacgg gccgtttggt tttcttgatg attacgaacc cggttttatt tgtaatcact
    14941 cggatcatca agggcgttac agctttgata atcaacctgc cgtcgcgttg tggaatttac
    15001 agcgtctggc gcagacattg tcgccatttg ttgccgtaga tggcctgaat gaggccctgg
    15061 acagctatca gcaggttttg ttgacgcatt atggacaacg gatgcggcag aaactgggct
    15121 tcatgacgga gcaaaaagag gataacgcgc tacttaatga attattcagt ctgatggcgc
    15181 gagagcgcag cgattatacc cgcacattcc gcatgctgag tctgaccgag cagcacagcg
    15241 cggcgtcacc gctacgtgat gagtttattg atcgtgcggc atttgatgac tggtttgccc
    15301 gttatcgggg gcgtttgcaa caagacgaga ttaccgatag tgagcgtcag caactgatgc
    15361 aaagcgttaa cccggctctg gtgttgcgca actggttggc gcaacgggcg attgaagcgg
    15421 cagaaaaggg tgatatgacg gaattgcacc gcctgcatga ggcgttgcga aatcctttca
    15481 gcgacagaga tgatgactat gtcagtcgtc cacccgactg gggtaaacgg ctggaagtca
    15541 gctgctcgag ttaaaggtta cttggtcaac agcagcttgc cagcctgagt tttacggagc
    15601 agatattctt gcccgtcatg atcgataatc agtttgccat ccggacctaa cagtgtctgg
    15661 ctggtaattc gccggatggg ttgcggggaa gcggtcttgt gactggcatc cgtttcaggc
    15721 gtgagttttc tgctatccgt ataacgcata aaaattaata ataatttata taacaatgat
    15781 tcttattatc aatattatgg cgggccagag caagcagtaa tttgttgaga agacaacaag
    15841 ttgcgcagaa atgcccatcc cgtcaggaat gggcacgcat tcagaagcga gtatctaccg
    15901 cagaggcgag tttttcgacc aggcgttcgg tatcttccca gcccagacac gggtcggtaa
    15961 tggattgacc gtaagtgagc ggctgaccgc cgacgatttt ttgcgttcct tcgcgcagga
    16021 aactttccgc cataattcca gcaatcgccg tagagccatt gcggatttgc tgacaaatat
    16081 cctcacaaac ttctaactgg cgacggtgct gcttctggca gttaccgtgg ctgaaatcca
    16141 ccaccagatg ttcaggtaaa tcaaactcgt gcagcgtatc gcaggctgcg gcgatatcct
    16201 cggcatgata attcggtttt ttgccaccac gcataataat gtggccatac gggttgccgc
    16261 tggtctgata gatggtcatc tgaccatttt tgtctggcga gaggaacata tggctggcgc
    16321 gggctgcgcg gatagcatcc acggcaatcc gcgtgttgcc atcggtacca tttttaaaac
    16381 ccaccggaca ggagagtgcc gaagccattt cgcggtggat ctgactttcg gtagtacgtg
    16441 cgccaatcgc gccccaactg attaaatcag caataaactg accggtcacc atatcgagga
    16501 actcagtcgc ggtagggacg cccagctcat ttacctgtaa aagtaatttg cgcgccagct
    16561 ccagaccgtg atttacccga tagctaccgt ttaaatctgg atcggagatg agtcctttcc
    16621 agccgacaac cgttcgtggt ttttcaaaat aggtgcgcat tacgatttcc agccgtggct
    16681 ggtactggtt gcgtagcgac tgcagacggg tggcgtactc catcgcagcg gtgagatcgt
    16741 ggatcgagca ggggccaata atgaccaaca gtcgcttatc ttcaccattc agtatttttt
    16801 caattctacg gcgggagtcg gtgacatggg tggcgacgcc aggcgttacg ggataccgta
    16861 gcgcgagttc ggcgggcgtt accaggctct caatacgcgc agtacggagt tcgtcagttc
    16921 tgttcattac aagtctcaga aatttgttgc ttctacggca ggactaccgg gaagtgatgc
    16981 gcatcacgat aaaccaatcg cagctgattt caagtgcgag gtgtgtcaca cctcgccggg
    17041 tggttagcat gataacaaaa aagtaagcca gtacactagt tctctagtac attcggcggc
    17101 taaggcccat gatatcgagg atcttggtgg caatctcttc taccgaataa ttggtactgt
    17161 taatccacgg gatctgattt ttacggtaca acgcttccac ttccgcgact tccatcctgc
    17221 actgacgaag cgaggcatag cggctgttct cccgacgttc ctcgcgaatc gccgccagac
    17281 gttccgggtc gatagtcagg ccgaacaatt tgtgctgaag tggtttgagc gacgcgggta
    17341 gcaccagatt atccatatcg tcggcaataa aggggtaatt tgccgcgcgg ataccaaatt
    17401 gcattgccag atacagactg gtgggggttt taccacagcg agaaacaccg agcaggatca
    17461 cctgagcctg gtccagattg cgcaacgaaa taccgtcatc gtgggcaagg gtgtaatcaa
    17521 tcgccgcaat gcgcgcatca tatttattga gattattagg gttaaggcca tgggtacgat
    17581 gagcaatcgg cgttggatcc agtttcatct cttgttgtag cggggcaacc agcgcctgaa
    17641 cgatatcctg gcaaaagcct tcactttgca agatgatagc gcgaatctcc ggcaacacga
    17701 tggagtagaa gaccagcggg cgcacacctg tctggtgata aattgcgtca atctgatcct
    17761 tcactgcccg tgcacggctc tcattttcga caaacggcag cgtaatgctg ctgatagtga
    17821 cgggaaattg tgacattact gcgtgtccta atacctccgc agttattgcc gtaccatcag
    17881 aaatataaaa aacgtggcga tcaacagcat tatccatttt gttcttcccg tgatgcagac
    17941 ataattctat gaaagcataa attaaaaacg cacagaagcg tagaacgtta tgtctggttt
    18001 ataaaatgaa acttaaattt tattttttat gaaaacagca tttcattttt atggtttcgt
    18061 ttataccgat ggtttatgtg gaaattgtcg aagagag
//
LOCUS       DAZFFE010000097        17815 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFFE010000097 DAZFFE010000000
VERSION     DAZFFE010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.097"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1906)
                     /locus_tag="ACHJ69_003628"
     CDS             complement(<1..1906)
                     /locus_tag="ACHJ69_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO5016526.1"
                     /translation="MSIQTESLMTDALCGMLAADISLSRPVSSSDEYDKLSRSYHMNR
                     RNLAMLARPESGPLLSSGTRPPRDTPVDLLVWSLAVPDFTYEQRSRVVSLISEFGSFS
                     YTVAYAFSRLVYQCGVSNAVAVLESVHRYAFINADGDRYSVGLFCTDSELAVAADSIV
                     HDCLCSSAIVSEDEGVWLANVLHYFYAVSGFDFLPCLKAYMTRYGISGLLKRMTSLKF
                     VTRFLRVVRDQRVNEVCRMLGILNRTTPYISDWHLALFESRKDKVTRWLKNNGVFDFS
                     GELLCTLEDAHNSTVSNPVNRVAELCVRGKAVCELAEDFGLQGFFVVLTTPSRFHPTT
                     SYRAGGRWCSRQNPNWLAAGCPTVKDSHQWLNRVWQNIQRKLNKAGIQLPGIRTVEPH
                     ADGTVHWNFLFYCQPYDAEAVLNIFRDEALADNPGEPGAQDHRIKIKEIDPGKGGFRY
                     IVKYITKMAGHADAKGTGHLDDIHSSRSFSDAVNRVACWQKTTRLRLFQFFGLPSVTA
                     YRQLRRFRIPFSPDDIAMKKITPEQVEQLEKIRLSCDAGDFRTYILLNGGFFCSERLI
                     RPYYFQQVQDGMPRLNVYGEICAPVISGFLFNKVPFITRVFGLFISAIKELSVPVFNS
                     CVRTGDTLHTR"
     gene            complement(2035..2988)
                     /locus_tag="ACHJ69_003629"
     CDS             complement(2035..2988)
                     /locus_tag="ACHJ69_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO5016527.1"
                     /translation="MTESVKVNVGIDVSKDFLDVHVGNVALRRVPNRSSGLSVLKRIL
                     SRYSVSLVLCESTGGYENLAVTYFQSLGYDVCVINPRQARDFARSMGRLAKTDRIDAQ
                     ILCRLADVIDASPERTRFIRPLTDERRQHLVSMVRRRRQLCDLMTAERNRRAKTDDYG
                     SESISTVLDFLTDEVARIDRDIAAHVGSCFSDISSLLQTFTGIGPVTASTLLGELPEL
                     GKLNRRQITALVGVAPFNRDSGYMRGRRRISGGRSSVRNVLFMAALSSVRFNPVLKAF
                     FTRLVAAGKPKKVALVACMRRMVCILNAMLRDGSRFEVAVG"
     gene            3187..3558
                     /locus_tag="ACHJ69_003630"
     CDS             3187..3558
                     /locus_tag="ACHJ69_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO5016528.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            3664..4062
                     /gene="fadM"
                     /locus_tag="ACHJ69_003631"
     CDS             3664..4062
                     /gene="fadM"
                     /locus_tag="ACHJ69_003631"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO5016529.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWHGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(4114..4809)
                     /gene="queC"
                     /locus_tag="ACHJ69_003632"
     CDS             complement(4114..4809)
                     /gene="queC"
                     /locus_tag="ACHJ69_003632"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO5016530.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(4874..6574)
                     /locus_tag="ACHJ69_003633"
     CDS             complement(4874..6574)
                     /locus_tag="ACHJ69_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO5016531.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDMGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSLRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLTGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            6674..7492
                     /gene="cof"
                     /locus_tag="ACHJ69_003634"
     CDS             6674..7492
                     /gene="cof"
                     /locus_tag="ACHJ69_003634"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO5016532.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            7645..8103
                     /gene="decR"
                     /locus_tag="ACHJ69_003635"
     CDS             7645..8103
                     /gene="decR"
                     /locus_tag="ACHJ69_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO5016533.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            8133..9905
                     /locus_tag="ACHJ69_003636"
     CDS             8133..9905
                     /locus_tag="ACHJ69_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO5016534.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRTMLAEAPVVIDGSDKVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMVGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAIDA"
     gene            9898..11679
                     /locus_tag="ACHJ69_003637"
     CDS             9898..11679
                     /locus_tag="ACHJ69_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO5016535.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSTLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     EIVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDANTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLVGEELAASVREEESLSA"
     gene            11860..12198
                     /gene="glnK"
                     /locus_tag="ACHJ69_003638"
     CDS             11860..12198
                     /gene="glnK"
                     /locus_tag="ACHJ69_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO5016536.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            12228..13514
                     /gene="amtB"
                     /locus_tag="ACHJ69_003639"
     CDS             12228..13514
                     /gene="amtB"
                     /locus_tag="ACHJ69_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO5016537.1"
                     /translation="MKIATIKTGLTSLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTVPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNING
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(13563..14423)
                     /gene="tesB"
                     /locus_tag="ACHJ69_003640"
     CDS             complement(13563..14423)
                     /gene="tesB"
                     /locus_tag="ACHJ69_003640"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO5016538.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPDERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            14641..15213
                     /locus_tag="ACHJ69_003641"
     CDS             14641..15213
                     /locus_tag="ACHJ69_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO5016539.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(15244..15555)
                     /locus_tag="ACHJ69_003642"
     CDS             complement(15244..15555)
                     /locus_tag="ACHJ69_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO5016540.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQLYRWN
                     Y"
     gene            15718..15814
                     /gene="ffs"
                     /locus_tag="ACHJ69_003643"
     ncRNA           15718..15814
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHJ69_003643"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            15934..16287
                     /locus_tag="ACHJ69_003644"
     CDS             15934..16287
                     /locus_tag="ACHJ69_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO5016541.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(16329..>17815)
                     /gene="pdeB"
                     /locus_tag="ACHJ69_003645"
     CDS             complement(16329..>17815)
                     /gene="pdeB"
                     /locus_tag="ACHJ69_003645"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO5016542.1"
                     /translation="GLSIWLAHQQVETSFIEELNTYSSRVAIRANKVATQGKDALQEL
                     ERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNVPQCSSLEHESPPDTFPEPGKIS
                     KDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPASFIDVIPYSSWQIDAAIIGNAHN
                     VVITSSDELAQGIITRLQKTPGEHIENNGIIYDILPLPEMNISIITWASTKMLQKGWH
                     RQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQDVIENRDICVHYQPIVSLANGK
                     IVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPLTLLIIRSVFEDMGDWLRQHPQQ
                     HISINLESTVLTSEKIPQLLREMINHYQVNPRQIALELTEREFADPKTSAPIISRYRE
                     AGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVDALEYKNVTPHIIKMAKTLKLKM
                     VAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDFLRWAEQHL"
BASE COUNT         4396 a         4434 c         4734 g         4251 t
ORIGIN      
        1 ggcgcgtgtg cagcgtgtca cctgtccgca cacaggaatt aaaaacaggc acagacagtt
       61 ctttaatcgc agaaataaac aaaccaaaaa cacgggtaat aaacggcacc ttattaaata
      121 aaaaaccgga tattacaggg gcgcagattt caccatagac attcaaacgg ggcatgccgt
      181 cctgaacctg ctgaaaataa tatggcctga ttaaacgttc agagcagaaa aaaccaccgt
      241 tcagaagtat ataggtacga aaatcacctg catcacagga aagacgaatt ttctcaagct
      301 gttcaacctg ttctggtgtg atttttttca ttgcaatatc atcaggcgaa aacggtatcc
      361 tgaaacgtcg caactggcgg taagcagtca cagaaggcag cccgaaaaac tgaaaaagac
      421 gaaggcgggt tgttttctgc cagcaggcaa cacgattgac cgcatcactg aatgaacggg
      481 atgagtgtat atcatcaaga tgaccagtgc cttttgcatc agcatgaccc gccattttgg
      541 tgatgtactt gacgatatag cgaaaaccgc cttttcccgg gtcaatctct ttaattttaa
      601 tgcggtggtc ctgagcaccc ggttcacccg gattatctgc aagtgcctca tcgcggaata
      661 tgttcagaac agcctcagca tcataaggct ggcagtaaaa caaaaaattc cagtgaacag
      721 taccgtctgc atgaggctca accgtacgga tacccggcaa ctgaatcccc gccttgttta
      781 atttacgctg aatgttctgc cagacgcgat taagccactg atgcgaatcc ttaaccgtag
      841 ggcacccggc agccagccag ttcggattct ggcgggagca ccaacggccc ccggctctgt
      901 aactggttgt cggatgaaac cgtgacggtg tggtaagcac aacaaaaaac ccctgaagac
      961 caaaatcttc agcaagctca cagacagcct taccccgaac gcaaagttca gccacacggt
     1021 taaccggatt tgaaaccgtt gaattatgag catcttccag tgtgcagagc agctcaccac
     1081 taaaatcaaa cacaccgtta tttttcagcc atcgggtaac tttatctttg cgactttcaa
     1141 ataaagccag atgccagtct gaaatataag gggtggtgcg gttaagtatt cccagcatgc
     1201 gacagacctc attcacccgc tggtcacgaa caacacgcag gaaacgagtc acgaacttaa
     1261 gcgaggtcat acgtttaaga agaccggaaa tgccgtaacg tgtcatgtat gctttcaggc
     1321 acggcaggaa atcaaaaccg gaaacggcat aaaaataatg cagcacgttt gccagccaga
     1381 ctccctcatc ctctgagaca atggcagaag aacacagaca atcatgcaca atactgtcag
     1441 ccgcaaccgc cagttcagaa tcagtacaga acagaccgac gctgtaacgg tcaccatcag
     1501 cattaataaa ggcgtaacga tgtacagatt caagaacggc aacagcgtta ctcactccgc
     1561 actgataaac cagacgcgaa aaagcataag caacggtata actgaatgaa ccaaactcac
     1621 tgattagcga caccacacgg gagcgctgct cataagtaaa atcaggtaca gccagcgacc
     1681 agacaagaag gtctacaggt gtgtcacggg gcggacgtgt gccggaagaa agcaaaggac
     1741 cggattcagg gcgcgccagc atggcaagat tgcgacgatt catgtgatac gagcgagaca
     1801 atttatcgta ctcatcagaa gacgaaaccg gacgggataa tgagatatcc gccgcaagca
     1861 tgccacaaag ggcatcagtc atgagggatt cagtttgtat actcataatc agttaccgga
     1921 tttcaggagg caaaaaatcg agcgcccaag aaacacaata cccacaccca aaaagaccaa
     1981 cataaccaaa cagaacagtt ccaaggtttc catcgtttac gaatctccgc acactcaccc
     2041 cacagccacc tcaaaacggg aaccgtcacg aagcatggcg ttaaggatac agaccatgcg
     2101 gcgcatacag gcaaccagtg ccaccttttt cggtttcccg gcagcaacaa gacgggtaaa
     2161 gaacgcctta agtaccggat taaaacggac ggatgacagc gccgccataa acaacacatt
     2221 ccgcacgcta gaacgcccgc cagagattcg acgacgacca cgcatataac ctgaatcacg
     2281 gttaaacgga gccacgccaa caagagccgt aatctgacgg cggttaagct ttcccagttc
     2341 aggcaattcc ccgagcaggg tgctggctgt tactggacca ataccagtga atgtctgaag
     2401 aagtgaggaa atatcagaga agcaggaacc cacatgcgcc gcaatatcac ggtcgatacg
     2461 ggccacttca tcggtcagaa aatcaagcac tgtactgata ctttctgaac cgtaatcgtc
     2521 cgttttggcc cggcggttac gctcagccgt catcaggtca cagagctgac gacgacggcg
     2581 aaccatcgac accagatgct gacgacgctc atcagtcagc ggtctgatga aacgggtacg
     2641 ctcaggcgat gcatcaataa catcagccag acgacaaaga atctgagcat caatccggtc
     2701 agttttggca aggcgaccca tggaacgggc aaaatcacgc gcctgtcggg gattaatcac
     2761 acagacatca tagccaagcg actgaaaata cgtcaccgct aaattttcat accccccggt
     2821 ggactcacac aggacaagag aaacactgta acgggacaaa atacgcttca gaacagacaa
     2881 accgctggag cggttaggaa cacgacgcag ggccacatta cccacatgta catccagaaa
     2941 atccttactg acatcaatac caacgttaac tttgacagat tcagtcatgt ttaacccttc
     3001 ctagtgtgcg aacttgattc ggtaaacgta attcgatcaa caaatacggg cttgaacaaa
     3061 aataagaacc atgcgctata ggcttgttta cgggctaaaa ccccaggaga aatcaggctg
     3121 catggttctt ttctgtacag caggaaggag tataaacaca gatcaaacac actaggagaa
     3181 aacagtatga aacacggaat taaagcactg ctcattaccc tgtccctggc ctgtgccgga
     3241 atgtctcata gcgcgctggc ggcagcttct gtggcgaaac cgacaacagt agaaaccaaa
     3301 gcggaagctc ctgcagcaca aagtaaagca gcagtaccgg cgaaagccag tgacgaagaa
     3361 ggcacccggg tcagcattaa taatgccagc gcggaagagt tagcccgcgc gatgaatggc
     3421 gttggcctga agaaagcgca ggcgattgtc agttatcgcg aagagtacgg tccgtttaaa
     3481 acggtggagg atctaaagca ggtgccgggg atgggcaatt cgctggtgga acgtaatctg
     3541 gcggtattaa ctctgtaatt aatttgcata gtggcaattt tttgccagac tgaagaggtc
     3601 ataccagtta tgacctctgt acttataaca gttcttataa caacaacgta aggttattgc
     3661 gctatgcaaa cacaaatcaa agttcgtgga tatcatctcg acgtttacca gcacgtaaac
     3721 aacgcccgct accttgaatt tctcgaagaa gcccgctggc atgggttgga aaatagcgac
     3781 agttttcact ggatgacggc ccataacatc gccttcgtcg tggtcaatat caatattaac
     3841 tatcgccgcc cggcggtatt aagcgacctg ttaaccatta ccagtcagtt gcagcaatta
     3901 aacggtaaaa gtggcatctt aagccaggtc attacactgg agccggaagg acaggtggta
     3961 gcggatgcgc ttattacgtt tgtttgtatt gatcttaaaa cgcagaaagc attagctctg
     4021 gaaggggaat tgcgcgaaaa gctggagcag atggttaagt aaacgttttg tggtgccgga
     4081 tgctcaagcc gcatccggcg acacccggaa taattacttc aacccggttt tctgcttcat
     4141 cgctgccatc accgtcggtt tatcggccag ataatgattc aaaccgttgg cacgtaaatt
     4201 acacgccgca caatgaccgc aaccgtcgcc tttaatgccg ttataacagg tcagcgtttc
     4261 gttacggact aaatccagct tgccgtaata atctgccagc gcccaggttt ccgctttatc
     4321 aatccacatc agcggcgttt caaaacgaat atctttcgcc atgcccaaac tgacggcatg
     4381 gtttagtgct ttcacaaact catcgcggca atccgggtag ccggagaaat ccgtttcgca
     4441 gacgccggta attacggctt ctgcttttac ctgatacgca tatattgccg ccagtgtcag
     4501 gaacaaaata ttacgccctg ggacaaacgt attcgggata ccatcggctt caggttcata
     4561 atcaggcacc ggaatgctgt cacgcgtcag gctgctgacc gccagctcgt tgagcagcgt
     4621 tacatcaagc accttatgcg cgcgtgcccc cagtttcagc gccagttcgc gtgccacgtc
     4681 gatttctgcg cgatggcgtt gaccgtaatc gaacgtcacg caatggacct catcatattg
     4741 ttgtaatgcc tgcaccagac aggtggtgga atcctggcct ccactgaaca caacgacagc
     4801 acgtttcata gataatcctg cttgaacaat aaaagcgtta tggtaacgcc tgcgattaac
     4861 ccggaccagc tattcattgc gacggcgcgg gaagccaggc ttcggtaaat tcaaaccagc
     4921 cgcgcggtgt cagtcgcaca ccgttcacgc cgggaggggc gctaatgcga tagtgatagt
     4981 tgaacagcgg cgtcagcgtc gcatctgcca ttaactggct aaaaaccgct ttcagggcat
     5041 taaatcggtt ttcctcatca ggcattactt gcactgcgtc cagtgtcgat tgcaaatgtg
     5101 catatgctgg agcgtcgaaa acatgtggcc acagtggatc gcaacgcagc cattgctcca
     5161 gggtatattc cggtgcttcg ccaattaatc tgtcgcccat catgaggtct gcgtgtgcca
     5221 gtagggtcgt gtcgtcccag ttttttgcgt tatgaaaaat aattgtgagt tcacagcctt
     5281 ccgctgccag tgtcgcctgt aggcgttctg ccatggtatg aagttctatc ggtaggtgat
     5341 aaaccagcgt caaggttttc ggtagtttaa cttcatccgg tacttgccaa tgcggaatag
     5401 tccagcctgt cagtaatgca tgactggcag tgatcaggtt ttctccgact tctaacgttt
     5461 gtaataaacc ggattgatga ataatggaga ttacttttcg cgcctgccag agggacagtc
     5521 ggagactttt gcgcaatgtt aaatagcaaa aacctaaact gatgccgcta ctgacctggc
     5581 tgacccgttg cagctcctcc ggtttgccaa tggtgatttg cacgggatgc cgacaactgg
     5641 ttcccatatc tttttcgaaa agcggcggag ttatccagta ctcaaccgct ttaagcagcg
     5701 gatgacgtaa atggtaataa tcatggctct ccaggcgcac cagttccgct gtgaattgtg
     5761 ttaagcgaaa aggacctgtg ccgatcagtg ggaattgcgg atgcgccaga tggctgcaat
     5821 agctcgccag tcggtgcgca agccagtaat cggggcgatg taaaaagaag gtcagacact
     5881 gcggatgggt gacttcaata cgcttcacgc taataaataa ttgatccagt gctggcagtt
     5941 gtaacagcat caataaccgc tggtgtaagt gggaggcttt tactgcatcg ccgttatgcc
     6001 agtgtaaggt tgagcgaaga taaaaatccc agcgtaaccc gtcagtagag gtttcccagt
     6061 gatgcgctaa atcgccaatc gggcgctggg tattattatc gaagcgggtc aggccggaaa
     6121 atatctgccc ggccagatgc tgctcggcac ggccgggcaa aaagcccggt tgtagcggtt
     6181 cgagcgggcg atagtaggga atacgcaacg tgggcgtatc gttttgccat tgtccgccca
     6241 taaacggctg taacagagtg cgcagctcac ctggggccag ttgcgccagc tccagcacat
     6301 cttgctgctt tccggtttcc agcgcctgtt ccatcatcgc attgcgtagc gattctggcg
     6361 tgaccagaaa gcgtaattgt ccgcgctttc cgcgtcctga ctgcgcctgc cactccagcc
     6421 atcccgcctc ctgtgcctgg cgtaacagcg tacgaacatg gcgttcgctg caaaaacagc
     6481 gttcggccag ttcgctgacg gtgacggttt gcggctttcc ggcggaaggt tgccacagac
     6541 gttgatactg gttaagacgg ttgagcagtc gcatataaac ccggaacaat attatttaac
     6601 tattcactat tacttccgta tatatcaggt gatactcaat caccattaac cgtgtcacag
     6661 agtggagaaa gaaatggctc gtctggcagc atttgatatg gatggcactt tattgatgcc
     6721 tgaccatcat ttaggtgaga aaaccctctc tactttggcg cggctgcgtg aacgcgacat
     6781 taccctcact tttgccacgg ggcgtcatgc gctggagatg cagcatattc tcggggcgct
     6841 atcgctggat gcatatttga ttactggcaa cggaacgcgc gtgcattctc tggaaggtga
     6901 acttttacat cgtgatgatt tacctgcgga tgtcgcggag ctggtgctgt atcagcaatg
     6961 ggatacccga gcaagcatgc atatcttcaa tgacgatggt tggtttaccg ggaaagagat
     7021 ccctgcgttg ttgcaggcat ttgtctatag cggttttcgt tatcagataa tcgatgtcaa
     7081 aaaaatgcca ctcggcagcg tcaccaagat ctgcttctgt ggcgatcacg acgatcttac
     7141 acgcttgcag atccagctat acgaagcatt aggcgagcgt gcacatttat gtttttccgc
     7201 cacggattgc ctcgaagtgc tgccggtggg ttgcaataaa ggcgctgcat tgacggtgct
     7261 gacccaacat ttaggtttat cgttacgcga ttgcatggcc tttggtgatg cgatgaacga
     7321 tcgcgaaatg ttaggcagcg tcggtagcgg atttattatg ggcaatgcga tgccgcaact
     7381 gcgcgcggag ctcccgcatt taccggtgat tggacattgc cgaaatcagg ctgtctctca
     7441 ctatttgacg cactggctgg actatccaca tctaccttat tcccccgaat aacgagatcc
     7501 cttccagcac cgggcaattg cccggttttt tttgcgttga atttgtcatt ttgtgccgtg
     7561 atgcttaaac cgcacagaat aaattgtcgc gatttcacct ttaaaataga attaaaagag
     7621 aaaaaaattc tctgtggaag ggctatgtta gataaaattg accgtaagct gctggccttg
     7681 ctacagcagg attgcaccct ctctttgcag gcactggctg aagccgttaa tctgacaacc
     7741 accccctgct ggaagcgtct gaaacggctg gaggacgacg gtatccttat cggcaaagtc
     7801 gccctgctgg atccggaaaa aataggcctc ggcctgaccg cctttgtgct gataaaaacg
     7861 caacatcaca gcagcgaatg gtattgccgc tttgtcacgg tggttaccga aatgccagaa
     7921 gtgctggggt tctggcgcat ggctggtgaa tacgattatc tgatgcgcgt ccaggttgcc
     7981 gacatgaaac gctacgacga gttttataag cgtctggtaa acagtgtacc ggggctgtcg
     8041 gacgtcactt ccagcttcgc gatggaacag attaaataca ccacttcttt acccatcgaa
     8101 taaatatcca gaatcaagtc aggacacaac gcgtgcgatt atttgctcaa ttaagctggt
     8161 atttccgtcg ggaatggcgt cgctatctcg gggctgtcgc cttgcttgtc attatcgcga
     8221 tgctgcaact ggttccgcca aaagtggttg gtattgttgt tgatggtgtg acagaacaac
     8281 actttactac cgggcagatc ctgatgtgga tcgccaccat ggtgctgatt gccgttgtgg
     8341 tttatctcct gcgttacgtc tggcgcgtat tgctgtttgg tgcgtcttat caactggctg
     8401 ttgaactgcg tgaagattat taccgccagc taagccggca gcatcctgag ttttacctgc
     8461 gtcatcgcac gggtgacctc atggctcgtg cgaccaatga cgtcgatcgc gtcgtgtttg
     8521 ccgccggaga aggggtgctg acgctggtgg attcactggt aatgggctgc gctgtgttga
     8581 ttatgatgtc tacgcaaatt agctggcagt tgaccttatt tgccctgttg ccgatgccag
     8641 tgatggcgat catgatcaag cgcaacggcg atgccttgca tgaacgcttt aagctggcac
     8701 aggcggcgtt ttccagtctt aatgaccgca cccaggaaag cctcaccagt attcgcatga
     8761 tcaaagcctt tggtctggaa gatcgtcagt cggcgttatt tgccgcggat gccgaagata
     8821 ccggcaaaaa aaacatgcgg gtggcgcgta ttgatgctcg tttcgacccg accatctata
     8881 tcgcgattgg tatggcgaac ttgctggcga ttggcggcgg tagttggatg gtggtacagg
     8941 gcagtttaac gctgggccag ctcaccagtt ttatgatgta tttaggtctg atgatttggc
     9001 caatgctggc gctggcatgg atgtttaaca ttgtggaacg tggcagcgcg gcgtatagcc
     9061 gaattcgtac catgctggcc gaagcgccag tcgttattga tggtagtgat aaggtgccag
     9121 aaggtcgtgg tgaactggat gtgaatattc gccagtttac gtatccgcag actgaccatc
     9181 ctgcgctgga aaacgtcaat ttcgccctga aacccggtca gatggtaggt atttgcgggc
     9241 cgactggttc cggcaaaagt accctgttgt cgctcattca gcgtcatttc gacgtcagcg
     9301 agggggatat ccgctttcat gatattcctc tgacgaagtt acaactcgat agctggcgta
     9361 gccgcctggc ggtggtcagc cagacaccgt tccttttttc cgacaccgta gcgaacaaca
     9421 tcgcgttggg ttgcccgaat gccacccagc aagagattga gcatgtcgcg cggttagcca
     9481 gcgtgcatga cgatattttg cgtttaccgc aaggttacga tacagaagtg ggcgagcgcg
     9541 gagtgatgct ttccggtggg caaaaacagc gtatctccat tgctcgtgcg ttattagtca
     9601 acgcggaaat cctcatcctt gatgatgcgc tttcggcagt agacggacgc acagagcacc
     9661 agatcctgca taacctgcgt cagtgggggc agggaagaac ggtaatcatc agtgcccatc
     9721 gcctttctgc actgacggaa gccagtgaaa ttattgtgat gcagcacgga catatcgccc
     9781 agcgtggcaa tcatgatgag ctggcgcaac aaagtggctg gtatcgcgat atgtatcgct
     9841 atcaacaact ggaggcggcg ctcgacgacg ctccggaaat tcgcgaggag gccatcgatg
     9901 cgtagtttta gccaactgtg gccgactctc aagcgcctgt tagcgtacgg ttcaccgtgg
     9961 cgtaaaccgt tggggattgc ggtcctgatg atgtgggttg cggcggcggc agaagtcagt
    10021 gggccgctgc ttatcagcta ttttatcgac aatatggtag cgaaaaataa cctgccgttg
    10081 aaagtggttg cagggctggc ggcggcgtat gttgggctgc aactgtttgc agccgggcta
    10141 cattacgcgc agtcgctgct gtttaatcgg gcggcagtag gcgtagtgca acagttgcgt
    10201 accgacgtga tggatgctgc gttacgccag ccattaagcg agtttgatac ccaacccgtc
    10261 gggcaggtga tttcccgcgt cactaatgac actgaagtga tccgcgatct ctacgttacc
    10321 gtagtggcaa ctgtcctgcg tagcgccgca ctggtgggcg cgatgctggt ggcgatgttc
    10381 agcctcgact ggcgaatggc gctggtggcg ataatgattt tcccggtggt gctggtggta
    10441 atggtgatat atcagcgtta cagcacgccg attgtccgtc gtgtgcgcgc ttatctggcg
    10501 gatatcaacg acggctttaa cgaaatcatc aatggcatga gcgttatcca gcagttccgt
    10561 cagcaggcgc gatttggcga acgtatgggt gaggccagtc gttcacacta tatggcgagg
    10621 atgcaaaccc tgcgcctcga cggttttctg ctgcgtccgc tgctgagtct gttttcatcg
    10681 ctcattcttt gtggcttgtt gatgctgttt ggcttctccg ccagcggcac cattgaagtg
    10741 ggcgtgttgt atgcgttcat cagttatttg gggcgactta acgaaccctt aatcgaactg
    10801 accacgcagc aggcgatgct gcaacaggct gttgttgctg gtgagcgcgt ttttgagctg
    10861 atggacggac cgcgccagca atatggcaat gatgatcgcc cgttgcagag cggcaccatc
    10921 gaagtcgata acgtgtcatt tgcttatcgc gatgacaatc tggtgctaaa gaacattaat
    10981 ctctctgtgc cttcgcgcaa ctttgtggcg ctggtcgggc ataccggcag tggcaaaagc
    11041 accctcgcca gtttattgat gggctattac ccgctaacgg aaggtgagat tcgccttgat
    11101 ggtcgtccat taagtacgct aagtcacagc gcgctgcgcc agggcgtggc aatggtgcag
    11161 caagatccgg tggtgctggc ggataccttc ctcgccaacg tgacgctggg acgtgatatc
    11221 tctgaagaac gcgtctggca ggcgctggaa atcgtgcagc tggcggagct ggcgcgtagc
    11281 atgagtgatg gtatttacac gccgcttggc gagcagggga ataatctctc agtcgggcaa
    11341 aagcaactgc tggcactggc gcgcgtgctg gtggagacgc cgcaaatcct gatccttgat
    11401 gaggcaaccg ccagcattga ctccgggact gaacaggcga ttcaacatgc tctggcggcg
    11461 gtgcgtgaac atactacgct tgtggtgatt gctcaccgct tatcgactat tgttgatgcc
    11521 aacaccattc tggtgcttca tcgtgggcaa gccgtggagc agggcactca ccagcaactg
    11581 ctggcggccc agggacgata ctggcagatg tatcaactgc aacttgtggg cgaagagctg
    11641 gcagccagcg tgcgtgaaga ggaatcattg agcgcctgaa tagcgcaata tttcatcgtt
    11701 ggtgcaaaaa tgtaacgcac tgtgcactgt catagtgcgt tttcattttc aatcttctta
    11761 acttcctgct ctctttctcg tttttcattt ttggcacacc gcttgcaata ccttcttcgt
    11821 gtagcagaac cattaccgaa ttctgaccgg aggggatcta tgaagctggt gaccgtgatt
    11881 atcaaaccat tcaaacttga agacgttcgt gaagcgttat cttccattgg tattcagggc
    11941 ctgaccgtca ccgaagtgaa aggtttcggg cgtcagaaag ggcatgccga gctgtaccgg
    12001 ggggcggaat acagcgtcaa cttcctgcca aaagtaaaaa ttgatgtggc gattgctgat
    12061 gaccaactcg atgaagtgat cgatatcgtc agcaaagcgg cttacaccgg aaaaattggc
    12121 gacggcaaaa tcttcgtcgc tgaattgcaa cgcgtcattc gtattcgtac cggcgaagcc
    12181 gacgaagcgg cgctgtaatc tctggcacac agcaacagga acgaaaaatg aagatagcaa
    12241 cgataaaaac tgggcttact tcactggcga tgctgccggg actggtaatg gctgcacctg
    12301 cggtggccga taaagccgac aatgcgttta tgatgatttg tactgcgctg gtgctgttta
    12361 tgaccgttcc ggggattgcc ctgttttatg gagggctgat tcgtggcaaa aacgtgctgt
    12421 cgatgctgac gcaggtgacc gtgacgtttg cactggtctg tattctctgg gtggtttacg
    12481 gctactcgct ggcgtttggc gaaggcaaca acttcttcgg caacattaac gggttgatgc
    12541 tgaaaaacat tgaactgacg gcggtgatgg gcagcattta tcagtatatc cacgtggcgt
    12601 ttcagggatc gtttgcctgc attaccgtag gcttgatagt tggggcgctg gcggaacgaa
    12661 tccgcttctc agctgtgttg atttttgtgg tggtatggct gacgctctcc tacattccga
    12721 ttgcgcatat ggtctggggc ggcggtttgc tggcttctca cggtgcactg gatttcgcgg
    12781 gtggcacggt ggtgcacatt aacgccgcaa tcgccggtct ggtgggcgcg tatctgatag
    12841 gcaaacgcgt gggcttcggc aaagaggcgt ttaaaccgca caacctgccg atggtcttca
    12901 ccgggactgc cattctctat atcggttggt ttggctttaa cgccgggtca gcgggcacgg
    12961 cgaatgaaat cgcagcactg gcatttgtga atactgtggt cgcaacggcg gcggcaattc
    13021 ttggctggat cttcggtgaa tgggcgctgc gtggtaagcc ttcactgctg ggagcgtgtt
    13081 caggcgcgat tgccggtctg gtcggcgtga cgccagcgtg tggctatatt ggtgttggcg
    13141 gcgcgttgat tatcggcgtg gtagctggtc tggcgggctt gtggggcgtt accatgctca
    13201 aacgcttgtt gcgggtggat gatccctgcg atgtcttcgg tgtgcacggc gtttgtggca
    13261 ttgtcggctg tatcatgacc gggatttttg ctgccagctc gctgggcggc gtgggcttcg
    13321 ctgaaggtgt gacgatgggc catcagttgc tggtacagct ggaaagcatc gccattacga
    13381 ttgtctggtc tggtgtggtg gcatttatcg gctacaaact ggcggatctg acggttggtc
    13441 tgcgtgtacc ggaagagcag gagcgagaag ggctggacgt caacagccac ggcgagaatg
    13501 cctataacgc gtaacaagca ctgcaaaaaa cagccggacg gttttcacct ccggctattt
    13561 ttttaattgt gattacgcat caccccttcc tgaacggtcg aggcaaccag cacgccgtcc
    13621 tgggtataaa actcaccgcg cacaaagccg cgtgcgctgg acgccgaggt gctttccacg
    13681 ctatacagca gccattcatt caaattaaac gggcgatgga accacatgga atggtcaatg
    13741 gtggcaatct gaatccccgg ttcgagaaaa ccgatgccgt gcggctgtag agctaccggc
    13801 aggaaattaa gatcagaagc ataaccgagc agatactgat gaacgcgcag atcatccggc
    13861 acgctaccat ttgcgcggat ccacacctga cgatgcggtt ctgcgacgtg acctttcagt
    13921 gggttatgaa actccaccgg acggacttcc agcggacgat cgcagatgaa tttatctttc
    13981 agcactggcg gcagcaggtg cgccagcgat tgggcgattt gcgtttctga aggcaggcca
    14041 tcaggcgctg gcgcggacgg cattgttttt tgatgttcga aacccgcttc tggtgcctgg
    14101 aaagaggcag tcatataaaa aatcggtttg ccgttttgaa tagcagcaac ccggcgggcg
    14161 ctgaagctgt taccgtcacg cagcgtttcg acatcataaa taatcggctt cttactatcg
    14221 ccagggcgaa gaaagtagct gtgaaaagaa tgtaccagcc gctcgtcagg gacggtctct
    14281 tttgcagcat acaaggcctg acccacgacc tggccgccaa atacctggcg taaacctaaa
    14341 tcttcactct ggccgcgaaa gagtccttcc tcaatttttt ccagatttaa caatgtcagt
    14401 aaatttttta gcgcctgact catataactc tccagtaaca aagctgccgc agcaagccaa
    14461 agtgagttga gtataacgca aatttgccac tggtccgatg ggtgcaatgg tctgaattac
    14521 gggctaatta caggcagaaa tgcgtgatgt gtgccacact tgttgacgtt actattttgt
    14581 taaccactct tccggcgagg aaagttagcc cgctggtgca ttgataataa ggagaagtga
    14641 atgaaactcg tgcacatggc cagtggttta gcggttgcga ttgcgttggc ggcttgcgca
    14701 gataaaagcg cggatattca gacgccagca ccggctgcaa atacgtctat ttcagcaaca
    14761 caacaacctg ctatccagca accgaatgtc tccggtaccg tttggatccg tcagaaagtc
    14821 gcactgccgc ctgatgccgt gctgaccgtg acactttctg acgcgtcgtt agccgatgcg
    14881 ccgtcgaaag tgctggcgca gaaagcggtg cgtaccgaag gtaaacagtc accattcagc
    14941 tttgttctgc catttaaccc ggcagatgtt cagccgaacg cgcgtattct gttgagtgcg
    15001 gcgattaccg tgaatgacaa actggtattt attaccgata ccgttcagcc ggtgatcaac
    15061 cagggcggaa ctaaagccga cctgacattg gtgccggtgc agcaaaccgc cgtgccggtt
    15121 caggccagcg gtggcgcaac gactaccgta ccttcaactt caccaaccca ggtgaatccg
    15181 tcttcggcag ttccagctcc aacgcaatat taagcgaagt taaccctctc cgcccggaga
    15241 gggttagtag ttccagcgat aaagctgcaa gtcgatttgc ccgcttcccg acaccatcac
    15301 accttctgcc agtaatgcct gtcgctgacg ctgtaaatcc ggtccggtca gtgaaatggt
    15361 gccgtggcga ttaaccaccc ggtgccatgg caaggtgctg ccttcgggga gacgctttaa
    15421 cacaccgcct acctggcgcg cggcgcgcgg cgatcccgcc agtttcgcca catcaccgta
    15481 agtggtgaca tagccttcgg gaatagcggc tacgatttgc cagacgcgct ggggaaatga
    15541 atcttctttt tccatctttt cttcctgagg taatttttca gcataatctg gaaaaacgcc
    15601 cgagtgaagc cgcattgcgc aagaaaccag catctggcac gcaatgggtt gcaattagcc
    15661 ggggcagcag tgataatgcg cctgcgcgtt ggttctcaac gctctcaatg ggggctctgt
    15721 tggttctccc gcaacgctac tctgtttacc aggtcaggtc cggaaggaag cagccaaggc
    15781 agatgacgcg tgtgccggga tgtagctggc agggccccca cccatttctg cctcccaccg
    15841 tttcgtcaaa aaatcccaac atggctaaac tttaaccacc actgacgtcg caagaattgt
    15901 ctggctgcgc agtacgcttc ggaggtatgt ctgatgaagt atgttgatgg ttttgttgtt
    15961 gccgttcctg ccgataaaaa ggatgcctat cgggaaatgg ccgctaaggc cgcgccatta
    16021 tttaaagagt tcggtgcgct tcgtattgtc gaatgctggg ccagcgatgt accggatggt
    16081 aaagtgaccg attttcgtat ggcggtgaaa gcggaagaga atgaagaggt tgtctttagc
    16141 tggattgaat acccttcaaa agaggtccgc gacgctgcta atcaaaagat gatgtcggac
    16201 ccacggatga aagagttcgg cgagtccatg ccgtttgacg gcaagcgaat gatctatggt
    16261 ggattcgaat caatcatcga cgagtaggta gcgtgacggg ctgtgcggcg tgccgtcagc
    16321 cctaaaattc acaaatgttg ctcggcccag cgtaagaaat cctctttcgg taatgccttg
    16381 ctgtagagcc agccctgacc gtagtgcacg ccatgctggc gtagccactc ttcttgttta
    16441 ctggtttcga ttccctccgc aaccattttc agtttcagag tttttgccat tttgatgata
    16501 tgcggcgtga catttttata ttccagcgca tcaacgaaag atttatcgat cttcagaatg
    16561 tcgacatcca gatcctgtaa atagcttaaa cttgaatacc ctgtaccaaa atcatcgaga
    16621 taaatttcat ggcccgcctc ccggtagcga gaaattatcg gggcgctggt tttcggatcg
    16681 gcaaactcgc gttcagtgag ttcaagcgcg atctgtctgg gattaacctg atagtgattg
    16741 atcatttcac gcagcaattg cgggattttt tccgaggtga gcacggtgga ttcaagattg
    16801 atcgaaatat gctgctgagg atgctgacgc agccagtcgc ccatatcttc aaagacgctt
    16861 cttataatca gtagcgtcaa tggctcagaa aggcccgttt gctgtgccag cggaataaaa
    16921 ctatctggtg acaaccaact accgtctgtc tgcggccagc gcgccagtgc ctcagcaccg
    16981 acaattttgc cattggctaa ggagacaatc ggctgatagt gcacgcaaat atcacgattt
    17041 tcgataacat cctgcagccg atgatgcggt gactggatac ggcgcaaaat acgcagcaca
    17101 aacatcgctg ccagcaggcc aatcaccaac ccgagcggta accaaataaa gacttgccga
    17161 tgccagcctt tctgcaacat tttcgttgaa gcccaggtga tgatcgaaat attcatctcc
    17221 ggtaagggca ggatatcgta aatgattcca ttattttcga tatgctcacc gggtgttttt
    17281 tgtagcctgg taataattcc ctgagcaagt tcatcgctgc tggttatgac aacgttatgg
    17341 gcattgccaa taatggcggc atcaatttgc catgagctat agggaatgac atcaataaag
    17401 gaagcggggt cgatcatgac gacataatgt gccgttccca tggcgaccat gtaacgaata
    17461 atgcctaaat cgttatgcga tgttaaccag acacgataac catctttcga aattttacct
    17521 ggctcgggga aggtatcggg cgggctttca tgctccagag acgaacactg gggaacgttg
    17581 ttatcgatat aaaccacttc ctgaatatag cgataactgt aagatacccg acgcatttcc
    17641 atgagatggg cttcgctaca ggcagcgcct tgccatcttt ccagctcttg cagcgcatct
    17701 ttcccttgtg tcgccacctt attggcccga atagcgacgc gggaggaata cgtattcagc
    17761 tcttcaataa atgatgtttc tacctgctga tgggccagcc agatgcttaa gccga
//
LOCUS       DAZFFE010000098        17747 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZFFE010000098 DAZFFE010000000
VERSION     DAZFFE010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.098"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..480
                     /locus_tag="ACHJ69_003646"
     CDS             <1..480
                     /locus_tag="ACHJ69_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO5016543.1"
                     /translation="GQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAA
                     YLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAF
                     TLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            636..2171
                     /gene="gadC"
                     /locus_tag="ACHJ69_003647"
     CDS             636..2171
                     /gene="gadC"
                     /locus_tag="ACHJ69_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO5016544.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDAKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKSKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2302..3621
                     /gene="digH"
                     /locus_tag="ACHJ69_003648"
     CDS             2302..3621
                     /gene="digH"
                     /locus_tag="ACHJ69_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO5016545.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTPQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3999..4163
                     /locus_tag="ACHJ69_003649"
                     /pseudo
     CDS             3999..4163
                     /locus_tag="ACHJ69_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            4188..6587
                     /gene="dosP"
                     /locus_tag="ACHJ69_003650"
                     /gene_synonym="pdeO"
     CDS             4188..6587
                     /gene="dosP"
                     /locus_tag="ACHJ69_003650"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO5016546.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPQYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCINEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTSFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDEGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWYDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGIGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(6777..7214)
                     /gene="osmC"
                     /locus_tag="ACHJ69_003651"
     CDS             complement(6777..7214)
                     /gene="osmC"
                     /locus_tag="ACHJ69_003651"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO5016547.1"
                     /translation="MTIHKKGQAHWEGDIKCGKGTVSTESAVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSISIDTTADVSLDKVDAGFAITKIAL
                     KSEVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            7559..7774
                     /gene="bdm"
                     /locus_tag="ACHJ69_003652"
     CDS             7559..7774
                     /gene="bdm"
                     /locus_tag="ACHJ69_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO5016548.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            7876..8013
                     /gene="sra"
                     /locus_tag="ACHJ69_003653"
     CDS             7876..8013
                     /gene="sra"
                     /locus_tag="ACHJ69_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO5016549.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            8170..9867
                     /gene="maeA"
                     /locus_tag="ACHJ69_003654"
     CDS             8170..9867
                     /gene="maeA"
                     /locus_tag="ACHJ69_003654"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO5016550.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            10001..11011
                     /gene="adhP"
                     /locus_tag="ACHJ69_003655"
     CDS             10001..11011
                     /gene="adhP"
                     /locus_tag="ACHJ69_003655"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO5016551.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPSVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     ARIVQEKAGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            11197..11475
                     /locus_tag="ACHJ69_003656"
     CDS             11197..11475
                     /locus_tag="ACHJ69_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5016552.1"
                     /translation="MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLA
                     DIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDAYILDYEDYH"
     gene            11475..11759
                     /gene="yddM"
                     /locus_tag="ACHJ69_003657"
     CDS             11475..11759
                     /gene="yddM"
                     /locus_tag="ACHJ69_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO5016553.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(11988..12641)
                     /gene="fdnI"
                     /locus_tag="ACHJ69_003658"
     CDS             complement(11988..12641)
                     /gene="fdnI"
                     /locus_tag="ACHJ69_003658"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO5016554.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPIQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGL"
     gene            complement(12634..13518)
                     /gene="fdnH"
                     /locus_tag="ACHJ69_003659"
     CDS             complement(12634..13518)
                     /gene="fdnH"
                     /locus_tag="ACHJ69_003659"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO5016555.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEGNHE"
     gene            complement(13531..16578)
                     /gene="fdnG"
                     /locus_tag="ACHJ69_003660"
     CDS             complement(13531..16578)
                     /gene="fdnG"
                     /locus_tag="ACHJ69_003660"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15991..15993),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO5016556.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDDTLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGIANGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            16810..17691
                     /gene="yddG"
                     /locus_tag="ACHJ69_003661"
     CDS             16810..17691
                     /gene="yddG"
                     /locus_tag="ACHJ69_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO5016557.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDQIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYAHGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         4374 a         4542 c         4403 g         4428 t
ORIGIN      
        1 ggtcagattg gtactttcgc catcaacttc tcccgcccgg cgggtcaggt gattgcacag
       61 tactatgaat tcctgcgcct cggtcgtgaa ggctatacca aagtacagaa cgcttcctac
      121 caggttgctg cttatctggc ggatgaaatc gccaaactgg ggccgtatga gttcatctgt
      181 acgggtcgcc cggacgaagg catcccggcg gtttgcttca aactgaaaga tggtgaagat
      241 ccgggataca ccctgtatga cctctctgaa cgtctgcgtc tgcgcggctg gcaggttccg
      301 gccttcactc tcggcggtga agccactgac atcgtggtga tgcgcattat gtgtcgtcgc
      361 ggcttcgaaa tggactttgc tgaactgttg ctggaagact acaaagcctc cctgaaatat
      421 ctcagcgacc acccgaaact gcagggtatt gcccagcaga acagctttaa acacacctga
      481 taacgtttaa cggtaacggt gtcccgaaac gaagccgttt cgggacaatt tccaaagtct
      541 gttcactggc attagcaacg gaaaatattg ttctgaatac gcttcagaac aaaacaggtg
      601 cggttccgac aggaataccg ttttaggggg ataatatggc tacatcagta cagacaggta
      661 aagctaagca gctcacatta ctcggatttt ttgccataac ggcatcgatg gtaatggctg
      721 tttatgaata ccctaccttc gcaacatcgg gcttttcatt agtcttcttc ctgctattag
      781 gcgggatttt atggtttatt cccgtgggac tttgtgctgc ggaaatggct accgtcgacg
      841 gctgggaaga aggtggcgtc ttcgcctggg tatcaaatac tctggggccg agatggggat
      901 ttgcagcgat ctcatttggc tatctgcaaa tcgccattgg ttttattccg atgctctatt
      961 tcgtgttagg ggcactctcc tacatcctga aatggccagc tctgaatgaa gaccccatta
     1021 ccaaaactat tgcagcactc atcattcttt gggcgctggc attaacgcag tttggaggca
     1081 cgaaatacac ggcgcgaatt gctaaagttg gcttcttcgc cggtatcctg ttacctgcat
     1141 ttattttgat cgcattagcg gctatttatc tgcactccgg tgcccccgtt gctatcgaaa
     1201 tggatgcgaa gaccttcttc cctgacttct ctaaagtggg caccctggtt gtatttgttg
     1261 ccttcatttt gagttatatg ggcgttgaag cttccgcaac ccacgtcaat gaaatgagca
     1321 accccgggcg cgactatcca ctggctatgt tactgctgat ggtggcggca atctgcttaa
     1381 gctctgttgg cggtttgtct attgcgatgg tcattccggg taatgaaatc aacctctccg
     1441 caggggtaat gcaaaccttt accgttctga tgtcacatgt ggcaccggaa attgagtgga
     1501 cggttcgcgt gatctccgca ctgctgttgc tgggtgttct ggcggaaatc gcttcctgga
     1561 ttgttggtcc ttctcgcggg atgtatgtca cagcgcagaa aaacctgctg ccagcggcat
     1621 tcgctaaaat gaacaaaaat ggcgtaccgg taacgctggt catttcgcag ctggtgatta
     1681 cttctatcgc gttgatcatc ctcaccaata ccggtggcgg taacaacatg tccttcctga
     1741 tcgcactggc gctgacggtg gtgatttatc tgtgtgctta tttcatgctg tttattggct
     1801 acattgtgtt ggttcttaaa catcctgact taaaacgcac atttaatatc cctggtggta
     1861 aaggggtgaa actggtcgtg gctattgtcg gtctgctgac ttcaattatg gcgtttattg
     1921 tttccttcct gccgccggat aacatccagg gtgactctac cgatatgtat gttgaattac
     1981 tggtcgttag tttcctggtg gtacttgccc tgccctttat tctctatgct gttcatgatc
     2041 gtaaaagcaa agcgaatacc ggcgtcactc tggagccaat caacagtcag aacgcaccaa
     2101 aaggtcactt cttcctgcac ccgcgtgcac gttcaccaca ctatattgtg atgaatgaca
     2161 agaaacacta accatgtctg aatggttata agacaaggga gcgataattc atcgctccct
     2221 ttttcgtgct tgttgtcgta ttgacttaac cgggataagt acgagaatga gcgcacatct
     2281 gtttaccgga aaccagcaca tatggatatc tgctcccgaa acgagaaatt agcgattaga
     2341 agaccagcga tactggttgc actggcactt ttactgtgta gttgtaaaag cacgcctcca
     2401 gagtccatgg tgacaccacc agcgggctca aagccaccag ccacgacgcc acaatcgtca
     2461 caaccgatgc gtggcatctg gctggccacg gtgtctcgcc tcgactggcc accggtttcc
     2521 tcggttaaca ttagtaaccc caccagccgg gcacgtgtac aacaacaggc gatgatcgac
     2581 aaactggatc atctgcaacg tctcggcata aacacggtct ttttccaggt caagccggat
     2641 ggtactgccc tgtggccatc gaaaattttg ccgtggtccg atcttatgac cggtaagatt
     2701 ggtgaaaatc cgggttacga tccgctgcaa tttatgctcg acgaagccca caagcgtggg
     2761 atgaaagtac acgcctggtt taacccctat cgcgtatcgg ttaatacgaa gcccggtact
     2821 atcagggaac tgaatagcac tctgtctcaa caaccggcga gcgtctatgt gcaacaccgc
     2881 gactggatca gaacgtccgg cgatcgcttt gtcctcgacc cgggcatccc tgaggttcag
     2941 gactggatca catcaatagt cgcggaagtg gtttcccgct atccagtaga tggcgtgcag
     3001 tttgatgact atttctatac tgagtcacca ggttcacggc taaatgataa cgaaacgtac
     3061 cgcaaatacg gcggcgcatt tgcgtcaaaa gcagactggc ggcgcaacaa tactcagcag
     3121 ttaattgcga aggtatcgca caccattaaa agcattaagc cggaagtcga atttggcgtt
     3181 agcccggcag gcgtgtggcg taaccgatca cacgatccgc tcggttccga tacccgaggc
     3241 gcggcagcct atgacgaatc ctacgctgat acccgtcgat gggtggaaca agggttgctg
     3301 gattacattg ctccccaaat ttactggcca ttctcacgga gtgccgcgcg ttatgacgtg
     3361 ttggcaaaat ggtgggcgga tgtcgttaaa ccgaccagga cccgcctgta tatcggtatc
     3421 gccttctata aagtgggtga accttcaaag atagagccag actggatgat taacggcggc
     3481 gtaccggaac tgaaaaagca gctcgatctt aacgatgctg tgccggaaat tagcggcaca
     3541 atcttgttcc gtgaggacta tctgaataaa ccgcagacgc aacaagcggt cagctatctg
     3601 caaagtcgtt ggggcagtta aactcccatt cttactttgt aacaagccgg tgctttaccc
     3661 actggcttgt taccctcttg aactatccca ctagcaatca tgcttaaata cgtcaaatta
     3721 accacgcaat taaccacact tagttaaaca gccataatcc ccctaataaa aatgtatgaa
     3781 tatgaaaaaa catacgatat tcgctgtcac cgaaaaataa ttacgatggg ctatactctt
     3841 ctgaagaaca gtagcgtaga ttgaaactgt cctaagtcgc ttatttcttt tcagtatatc
     3901 ttcatatttc aggagaatat ggtgcagtta gtcgatctcg cacgttgtgt ttggttgatt
     3961 ttggcctgcg gtttaaccat aaaggtcgac attattttaa agccgcaaac ggcgaggata
     4021 ttgccctctc actttccatc ggtgccgcca tgtttaatgg tcatcctgac tatgagcgcc
     4081 tcattcaaat agccgatgaa gctctgtata tcgccaaaag acgaggtaga aaccgtgttg
     4141 aactctgtaa agccagtctt tagatgcgcc aggatgcaga ggtaatcatg aagctaaccg
     4201 atgcggataa tgccgccgat ggcatttttt tccccgccct tgagcaaaat atgatgggcg
     4261 cggtgttaat taacgaaaat gatgaagtga tgtttttcaa ccccgccgca gagaagctct
     4321 gggggtacaa acgtgaagaa gtcattggca ataacattga tatgctgatt ccgcgggatt
     4381 tgcgtcctgc gcatcctcaa tacattcgtc acaaccgtga aggtggtaaa gcgcgcgtcg
     4441 aggggatgag tcgggagctg cagctggaga aaaaagacgg cagtaaaatc tggacccgtt
     4501 ttgcgctatc gaaagtgagc gccgagggga aagtttatta cctggcgctg gtacgggatg
     4561 ccagcgtaga aatggcgcaa aaagaacaga cccgacaatt gattattgcc gttgaccatc
     4621 tcgaccgacc ggtgattgtc ctcgatccgg aacgccatat tgtgcagtgc aatcgcgcat
     4681 ttaccgaaat gtttggttac tgcattaacg aagccagcgg tatgcagccc gatacactcc
     4741 tgaacattcc agaattccct gctgataacc gtattcgttt acaacagttg ctatggaaaa
     4801 ccgcccgcga tcaggacgaa tttctgctgt tgacgcgcac cggtgaaaaa atctggatta
     4861 aagcctctat cagcccggtt tatgacgtgc tcgcgcatct gcagaacctg gtaatgacat
     4921 tctcggatat cacagaagaa aggcaaatcc gccagcttga aggcaatatt ctcgccgcca
     4981 tgtgcagcag cccgccattt catgaaatgg gggaaatcat ttgtcgtaac atcgaatctg
     5041 ttctcaacga atcgcatgtt tcgctgttcg cgctgcgcaa cgggatgccg atacactggg
     5101 cgtcatcttc gcacggtgca gaagttcaaa atgcgcaaag ctggtcagcg accattcgtc
     5161 agcgcgatgg cgcgcctgcg gggatcctgc aaattaaaac ctcgtcagga gcagaaacca
     5221 gcgcctttat cgaacgcgtg gcagatatca gccagcatat ggccgcgctg gcgctggaac
     5281 aggaaaaaag ccgtcagcat attgaacaac tcatccaatt tgatccgatg accggtctgc
     5341 caaatcgcaa taacctgcac aattacctcg atgacctggt cgacaaagcc gtctctccgg
     5401 tggtgtatct catcggtgtt gaccatattc aggatgtgat tgatagcctt ggctatgcgt
     5461 gggccgatca ggcattgctg gaagtggtca atcgctttcg tgaaaaactc aaaccggatc
     5521 agtatctctg tcgtattgaa ggtacgtctt ttgtcctcgt gagcctggaa aacgatgtca
     5581 gtaacattac ccagatcgcc gatgagctac ggaatgtggt cagcaagccg ataatgattg
     5641 acgataaacc attcccgctc accttgagca ttggcatcag ctacgacgag ggtaaaaacc
     5701 gcgattactt gctctccact gctcacaatg caatggattt tattcgcaaa aatggcggta
     5761 acggctggca gttcttcagc ccggcgatga acgaaatggt aaaagagcgt ttggttttag
     5821 gcgcagcgct aaaagaagcg attagcaata accaacttaa actggtttat cagccgcaaa
     5881 tcttcgcaga aacgggtgaa ctgtacggca tcgaagccct tgctcgctgg tacgatcccc
     5941 tgcatggtca tgtgccccct tcacggttta ttcctctcgc agaagagatt ggtgaaatcg
     6001 aaaatattgg gcgctgggtc atcgcggaag cttgccgtca gttagcagaa tggcgtagcc
     6061 agaatattca tatcccggcg ttatccgtta acttgtctgc gctgcacttt cgcagtaatc
     6121 agctgcctaa tcaggtatct gatgcaatgc acgcctgggg tattgacggc caccagctga
     6181 cggtggaaat cacggaaagc atgatgatgg aacacgatac cgaaatcttt aagcgcattc
     6241 agatcctgcg cgatatgggc atcggtttat cggtagatga ttttggtaca ggcttttccg
     6301 gattatcccg cttagtcagt cttccggtaa cggaaatcaa aattgataaa agttttgtcg
     6361 atcgttgtct gaccgaaaaa cgcatccttg ccttgctcga agccattacc agcattgggc
     6421 aaagcctcaa tttaaccgtc gtggcggaag gcgtcgaaac caaagagcaa tttgagatgc
     6481 tacgcaagat ccactgtcgc gttattcagg gatatttctt ttcccgcccc ctgcccgccg
     6541 aagaaattcc aggttggatg agcagcgtgt taccgctgaa aatctgataa attcctctcg
     6601 cccgcactcg cgggttttta tttaacgtga cactgtcact taaatactgt gatttccgct
     6661 acgtttagtt ggacaacgac aacgcatagc catgtcagaa caatcccttc tcagcctgat
     6721 gtgattgtgc tgacgcatca ggcattcacg cgcctgacgc gtcatccggc aacgctttac
     6781 gatttcaact ggtaatccag cgtaatttcc gctttcagta cctgagaaac cgggcatcct
     6841 gcttttgctt tctggataat gccgtcaaag gtggaggcat caatccccgg caccgcaact
     6901 tcgctcttca gtgcgatttt agtaatcgca aaaccggcat ccactttatc cagcgagaca
     6961 tcggcggtgg tatcaatcga tatcgatgtt ggcgtgaatc ccgcttcccc cagcattaat
     7021 gaaagcgcca ttgagaaaca tgcggcatgc gctgcgccaa tcagttcttc agggttggtt
     7081 cctttttcgc cttcaaaacg cgtgttaaat ccatacggtt gttggttcag cacggcgctc
     7141 tcagtagata ctgttccctt cccgcatttg atatcgcctt cccagtgtgc ctgacctttc
     7201 ttatggattg tcattgttgc tctcctgtgg gcttaaaaag gaaattatag actacgtgag
     7261 aaacagcacg aggataagca ggataatccg aataaaatcc ggcgaaaata taattcctgc
     7321 acttaaatta aattcccata atagataacc ggaaatatat aactcaagag atattgcatc
     7381 aatatcaatc aactaatcac aatgataata cctatcaata tcatggagtt aaaatataaa
     7441 aatcatcaac caggactaat cttaacaacg aagcggcaaa tatttgccat gctgaatgtg
     7501 cgttataaat ctgcgatccg tagcagacac cataaataca cagacacgga gaatcactat
     7561 gtttacttat tatcaggcag aaaattcaac agcagaaccc gctctggtta atgccattga
     7621 gcaaggcctt cgggcagagc acggtgttgt cactgaagat gacattctca tggagctgac
     7681 caaatgggtg gaagcatctg ataacgacat cctcagtgat atctaccagc aaacgatcaa
     7741 ttatgtggtc agtggtcagc accctacgct ttaaggtgct atgcttgatc ggcaacctaa
     7801 tttaggggtt tagcacgtgt ttcttcgcta cggcgatgtt gtccttaaaa ctagcaacag
     7861 gattgaggag ttaaaatgaa atcgaaccgt caggcacgtc atattcttgg actggaccat
     7921 aaaatttcta accagcgcaa aatagttacc gaaggtgaca aatccagcgt ggtaaataat
     7981 cccaccggca gaaaacgccc cgctgaaaag taattcataa ccatcagtcc tcaatgacga
     8041 ttaaacacca ttgcctgcgc aatggtgttt ttgtttttat ctgctttata cttggtactt
     8101 gacgccctgg cggtaatgca aagacgataa aagcccccag ggatggatat tcaaaaaaga
     8161 gtgagtgaca tggaaccaaa aacaaaaaaa cagcgttcgc tttatatccc ttacgctggc
     8221 cctgtattgc tggaatttcc gttgttgaat aaaggcagcg ccttcagcat ggaagaacgc
     8281 cgtaacttca acctgctggg gttactgccg gaagtggtcg aaaccatcga agaacaagcg
     8341 gaacgagcat ggatccagta tcagggattc aaaaccgaaa tcgacaaaca catctacctg
     8401 cgtaacatcc aggacactaa cgaaaccctc ttctaccgtc tggtaaacaa tcatcttgat
     8461 gagatgatgc cagtcattta caccccaacc gtcggcgcag cctgtgagcg tttttctgag
     8521 atctaccgcc gttcacgcgg tgtgtttatc tcttaccaga accgccacaa tatggacgat
     8581 attctgcaaa acgtaccaaa ccataatatc aaagtgattg tggtgactga cggcgaacgt
     8641 attctggggc ttggtgacca gggcatcggc gggatgggca ttccgatcgg taaactgtcg
     8701 ctctataccg cctgtggcgg catcagtccg gcctataccc ttccggtggt gctggatgtc
     8761 ggcaccaaca accaacagct gcttaacgat ccgctgtata tgggctggcg taatccgcgt
     8821 atcactgacg acgaatacta tgaattcgtt gatgaattta tccaggctgt gaaacaacgc
     8881 tggccggacg tactgttgca gtttgaagac ttcgcacaaa aaaatgcgat gccgttactt
     8941 aaccgctatc gcaatgaaat ttgttctttt aacgatgaca ttcagggcac cgcggcggta
     9001 acagtcggca cactgatcgc agccagccgc gcggcaggcg gtcagttaag cgagaaaaaa
     9061 atcgtcttcc ttggcgcagg ttcagcggga tgcggcattg ccgaaatgat catcgcccag
     9121 acccagcgcg aaggattaag cgaggaagcg gcgcggcaga aagtctttat ggtcgatcgc
     9181 tttggcctgc tgaccgacaa gatgccgaac ctgctgcctt tccagaccaa actggtgcag
     9241 aagcgcgaaa acctcagcga ctgggatacc gacagcgatg tgctgtcact gctggatgtg
     9301 gtgcgcaatg taaaaccaga tattctgatt ggcgtctcag gacagaccgg actgtttacg
     9361 gaagagatca tccgtgagat gcataaacac tgtccgcgtc cgatcgtgat gccgctgtct
     9421 aacccgacgt cacgcgtgga agccacaccg caggacatta tcgcctggac cgaaggtaac
     9481 gcgctggtcg ccactggcag tccatttaat ccagtggtat ggaaagataa aatctaccct
     9541 atcgcccagt gtaataacgc ctttattttc ccgggcatcg ggctgggtgt tattgcttcc
     9601 ggcgcgtcac gtatcaccga tgaaatgctg atgtcggcaa gtgaaacgct tgctcagtat
     9661 tcgccgctgg tcctgaacgg cgaaggtctg gtactgccgg aactgaaaga tattcagaaa
     9721 gtatcccgcg caattgcgtt tgcggttggc aaaatggcgc agcagcaagg cgtggcggtg
     9781 aaaacctccg ccgaagctct gcaacaggcc attgatgata atttctggca agccgaatac
     9841 cgcgactacc gccgtacctc catctaagcc tgcgcccggt agtgaaggct accgggcaat
     9901 ttccctctcc ctttttcaga tctcatccat actgggtagt ggcgaataaa tctcatttgc
     9961 ctcacctgct atgcagaaca tcatccgaaa aggaggaact atgaaggctg cagttgttac
    10021 gaaggatcat catgttgacg ttacggataa aacactgcgc tcactgaaac atggcgaagc
    10081 cctgctgaaa atggagtgtt gtggtgtatg tcataccgat cttcatgtta agaatggcga
    10141 ttttggtgac aaaaccggcg tgattctggg acacgaaggt attggtgtgg tggcagaagt
    10201 tggccctagt gttacttcat taaaaccagg cgatcgtgcc agcgtggcgt ggttctatga
    10261 aggatgcggt cattgcgaat actgtaacag tggtaacgaa acgctctgcc gttcagttaa
    10321 aaatgccgga tacagcgttg atggcgggat ggcggaagag tgcatcgtgg tcgccgatta
    10381 cgcggtaaaa gtgccagatg gtctggactc ggcggcggcc agcagcatta cctgtgcggg
    10441 ggtcactacc tacaaagccg ttaagctgtc aaaaattcgt ccaggacagt ggattgctat
    10501 ctacggtctt ggcggtctgg gtaacctcgc cctgcaatac gcgaagaatg tctttaacgc
    10561 caaagtgatc gccattgatg tcaatgatga acagttaaaa ctggcaaccg aaatgggtgc
    10621 agatttagcg attaactcac gcaccgaaga cgccgccaga attgtgcagg agaaagccgg
    10681 tggcgctcac gcagcggtgg tgacagcggt agctaaagct gcgtttaact cggcggtgga
    10741 tgctgtccgt gcaggtggtc gtgtcgtggc tgtcggtctg ccgccggagt ctatgagcct
    10801 ggacatccca cggctggtgc tggatggtat tgaagtggtc ggttcgctgg tcggcacgcg
    10861 tcaggatcta accgaagcct tccagtttgc cgccgaaggc aaagtggtgc cgaaagtcgc
    10921 cctgcgtccg ttagcggaca tcaacaccat ctttaccgag atggaagaag gcaaaatccg
    10981 tggccgtatg gtgattgatt tccgtcgcta agaggtcttt gctacgactg ccatgttcgg
    11041 gtcgcagcct gttttgcgag acgctttccg caatttcact cttcttcttt tcctccccca
    11101 tctttcccct cacaattcgc gaaaacgtag cgaaatttgt tgcgctgtac ggaacaatag
    11161 tgatagtgtt cctttcgagg gaacaaggat gaagagatga taatgaattt cagacacaag
    11221 ggattgcgtg acttgtttct tctcggcaaa acctccggcg ttataccgac gcaagtcaaa
    11281 cgattacgcc accgactcgc tgtgattgat gcagcatgct gtcttgctga tatcgatatg
    11341 cccggttacc gactacatcc gttaagcggc gatcgcgatg gaatttgggc gatatctgtc
    11401 tcgggcaact ggcgaatcac atttgaattc gtcaatggcg atgcatacat actggattac
    11461 gaggactatc actgatgaaa atggccaatc atccccgccc gggggacatt attcaggaat
    11521 cactggacga acttaatgtc agcctgcgcg agtttgccag agcaatggaa attgcgccct
    11581 caacggcaag ccgattgctg accggaaaag cagctttgac gccagaaatg gcaataaaac
    11641 tttccgtggt gatcggcagt tcgccgcaaa tgtggctgaa tctgcaaaat gcctggagtc
    11701 tggcagaggc agaaaaaaca gtggatgtgt cgcgactgcg ccgtctggtt acgcaataaa
    11761 aaaatggcac cttataaagc gccatttgaa actgatgaca aacgcaacat tgcccgatgc
    11821 gctacgctta tcaggcctac acgttcactg caatctattg gatttgaacg gtttgtaggc
    11881 cggataaggc gttcacgccg catccggcat gaacaaagtg tacgttgtct acaataaaaa
    11941 aatggcgcct tttacagcgc cattttcagc ttaaagctca aaagagatta taacccttct
    12001 tcactctctt ttttcgcttc tgccttctcg atttcacgat accagcgcgg atggtgtttc
    12061 ttcgcccagc gacggctcac cttcccttcg atcatccctt taatcgatcc tttcacccag
    12121 aatgccatat acatatggat caggatggcg tggatcagga tgatacccgc agccgcatgg
    12181 atcagcaggc tataacggac aacctgtatc ggaaagtact gcgcaaagta cggacgccag
    12241 ataatcactc cggtcaccag cagcacgaaa atcatgctca tgattgacca gaacatcatc
    12301 ttttgcccgg cgttgtactt accgacatcc gccactttgt gctcattgcc ttttaatact
    12361 tcgacaatgt tcaacagcca cggaatatct ttcttatccg ggatgttgtg atgcacaaaa
    12421 cgcacaaaca taaacatcag cgcgacgaaa atcgcaatgc cgaagaacgg gtgcaaaatg
    12481 cgtcccatct gcggcgtacc gaaggtttgc gtcagccatt gcagcgtcgg gaagaagaac
    12541 gaaatcccgg acagcgccac caggaagaag caaatcacca cggtccagtg acaggcgcga
    12601 tcaataaatt tggtgcgcac aatcattttc gacttactca tgatttccct cctcgtcatc
    12661 gtccacttcc ttattcgggc caataccgat gtagtggaaa atcaacccgg caaaagtggc
    12721 aataaagcca gccgctgcca gcggtttcaa cgcgcctttc cacaggctta ccgaggtatc
    12781 gatcttcgga tctttcggca gaccatgata cagctccggc tgatcggcat gatgcagcac
    12841 gtacataacg tgcgtaccac cgaccccttc cgggttgtag acgccagcat gttcgtaacc
    12901 acgcgctttc agtttcgcta cgcgctgttc cgccagctcc agcatctcct tcttggtgcc
    12961 gaagtggata gccccggtcg gacaggtttt cacacaagcc ggttcctggc cgacgctgac
    13021 gcgatcgacg cagagcgtgc atttatatac ccggttatcc tctttgttga ggcgcggaat
    13081 attaaacgga cacccggcaa tgcagtaacc acagccgatg cagttttccg actggaaatc
    13141 gacaatcccg ttagcgtact gaatgattgc accagcagac gggcatgcct tcaggcagcc
    13201 cggatcttca cagtgcatac agccatcttt acggatcagc cactccagct tgccgttctg
    13261 ttcggtttcg ctaaagcgca tcaccgtcca ggacttggcg ctcagatcgg cggggttatc
    13321 gtaaaccccg acgcagtgcc ccacttcatc acggatgtcg ttccactccg aacacgccac
    13381 ctgacaggct ttacagccga tacaggtgga aacgtcgata agtttcgcga cttctgcttt
    13441 gtaatcacgc acctgagaag gcggcgtgat ggagttagtt gcggaccttt tgataatgtc
    13501 ctgcgtttcc atagccatct gttcgccccc ttacgccttc tcgatgttta ctaagaacgc
    13561 tttatattcc ggcgtttgcg agtttgcatc accgacattc ggcgtcagag tgttagcgat
    13621 ataaccttta cgcgcgacac cctcaaagcc ccagtggatt ggaataccca ccgtttcaac
    13681 ctgctgaccg tttacattca gcggtttcag acggcgcgtt accacagcca ccgcgcggat
    13741 aaagccacgc ttgctggaga cagtgacacg atcgccattg gcaatgcctt tcgccgccgc
    13801 cagcgtttcg ctgatttcca caaactgttc cggctgagca attgcgttga gcaatgcgtg
    13861 cttggtccag gtgtggaagt gctccgtcag acgataggtc gtacccacat acgggaactg
    13921 ctctttttta cccatccgca gcgcgtcttg ttcatacaga cgaacaaccg ggttagacac
    13981 cacgttcgga tgcaacgggt tagtgcccag cggcgtttca attggctcgt agtgttccgg
    14041 gaacggacct tccgccattt tgttgatggc aaacaggcgt cccatccctt ccggctgcat
    14101 gataaacggc ccggttggcg tacccggtgc ggcattgccg aagtccggaa tatcgttacc
    14161 cgtccacttg ctaccgttcc actggatcag catccgtttc ggatcccacg gtttaccgtt
    14221 gatatccgcc gaagcacggt tgtagagtac gcgacggttg agcggccacg cccaggccca
    14281 tcccagcgta ttccccagac cggacgggtc ggagttatcg cggttagcca tctggttgcc
    14341 ctgctctgtc cagctaccgg tgtagatcca gcaagacgat gcggttgtac cgtcatcacg
    14401 cagatgcgca aagctactca gcaactgacc tttcttcgca atcagcacgc cattggcatc
    14461 atagagatct tccagcgcat agccgttgtt ctctttagct acttcgtcag attgcggttc
    14521 gtgcggctgc ttgtagttcc agctcatctt catcagcggt tctacgcctt taccaccttc
    14581 ggcctggtac agctcgcgca gatgatggta gatacccgcc agaatttcgc cgtcgttacg
    14641 cgcttcgccc ggcgcgtcct gacctttcca gtgccactgc aaccagcgac cggagttagc
    14701 gatagaacca tcttcttcag caaagcaggt cgaaggcaga cggaatactt cagtctgaat
    14761 agatgccgga tcgacatcgt tcgactcgcc gtggttctgc cagaaggtag aggtttcagt
    14821 caccagcgga tcgataacca ccatgtactt cagcttgctc aggcagctca ccactttgtt
    14881 tttgtccggg aaggacgcaa ccgggttaaa gccctggcag aaataaccgg tgactttacc
    14941 ttcatccatc atgttgaaat acttgatgac gtcgtaggtc tggtcccact tcggcagcca
    15001 gtcgtagccc cagttgttct ctttctgcgc ggcatcgccg tagaaagatt tcatcaggct
    15061 aacgaaaaac ttcggatagt tgctccagta gttcacctga tcagccagcg tcgctttcgg
    15121 cgtgttcgct tccagatacg actgcaaatc aacctgtttt tctgacggca gcgtcaggta
    15181 acctggcagg ctggtagaga gcaggcctaa gtcagtcagg ccctgaatgt tggagtgacc
    15241 acgcaatgcg ttcacgccgc caccggccat acccatgtta ccgagcagta actggatcat
    15301 cgccatcgta cggatgttct gcgcacccac ggtgtgctgc gtccagccca gcgcgtacag
    15361 gaaggtggtt gtgcgatccg gtgcgctggt ggaggccagc acttcacaca ctttcaggaa
    15421 gtcggctttt ggcgtaccgc aaatgttttc tactacgtcc ggcgtgtagc gggaaacgtg
    15481 cgctttcagc agattccata cacagcgtgg atgagtcagc gtgtcatcgc gtttcgcata
    15541 gccgttttca tcgaactgat agttccagga cgacttatcg tattgacgtt tttcagcgtc
    15601 gtagccgctg aacagaccgt cttcgaaagc aaaatcatca cgcaccagca ggctggcgtt
    15661 ggtgtaatgc ttaacgtatt cggcgttgat tttgttgttt tcgatcaggt agcgcaaaac
    15721 gccagacagg aacgtaatgt ccgtaccgga acgaataggt gcgtaaatat ccgccacaga
    15781 agcggtacgc gtaaaacggg gatcgacaac gatcaaggtt gcatcgttgt tgtttttcgc
    15841 ttccatcgcc cagcggaaac cgacgggatg cgcttcagca gcgttaccgc ccatcaccat
    15901 tacgacgtta gcgtttttga tatccaccca gtggttggtc atcgcaccgc gaccaaatgt
    15961 tggagcaaga cttgctaccg ttggtccgtg tcagacgcgc gcctggttgt ctaccgccag
    16021 catcccgagg gagcgggcaa atttttgagt cagcatcccg gtttcgttgc tggcaccgga
    16081 agcacacagc ataccggtag aaagccaacg gtttaccgtt acgccctgct cgttcttttc
    16141 aataaagtta gcgtcacggt cggctttcat cagcttcgca atacgggaga atgcttcttc
    16201 ccagctaatg cgctgccatt tgtcagaacc tggcgcacga tattccgggt agcgcagacg
    16261 gttttcgctg ttgacgtaat ccagcaaacc ggcccctttc gggcacagcg caccacggct
    16321 taccggatga tccgggtcac cttcaatgtg ataaatcgct tctctggcgt ttttcgcgcc
    16381 atcacccagg ctatacatca atagcccgca acctacggaa cagtatgtgc aggtgttacg
    16441 gatctctttc gcgcgtaata atttgtagtt tcgcgcctga gccagtgctt gcttcggggc
    16501 aaagcccaat gctgctactg ttgttcccgc cataccgccc gcgcagattt taaaaaattg
    16561 tctgcgactg acgtccattg ctttcctctt ttttcagggg tattactgcg cgaggaaact
    16621 aacagtaaaa ttctgaatct ttttgcgcga aatcaaaaag aacacgtgtt tgccacaaaa
    16681 taatccgcct gcgcaaattg acctacctca atagcggtag aaaaacgccc cactgcctga
    16741 cagggcagtt aaaaaaatgc tataaaattc agcttaattt ttaacggcaa gagagacaaa
    16801 acagcgagca tgacacgaca aaaagcaacg ctcatagggc taatagcgat cgtcctgtgg
    16861 agcacgatgg taggattgat tcgcggtgtc agtgagggac tcggcccggt cggcggcgca
    16921 gctgctatct attcattaag cgggctgctg ttaatcttca cggttggatt tccgcgtatt
    16981 cgacagatcc cgaaaggcta tttactcgcc gggagtctgt tattcgtcag ctatgaaatc
    17041 tgtctggctc tttccttagg gtatgcggcg acccgtcatc aggcgattga agtggggatg
    17101 gtgaactatc tatggcccag cctgacaatt ctctttgcca ttctgtttaa tggtcagaaa
    17161 accaactggc tgattgtacc aggattatta ttagccctgg tcggcgtctg ctgggtgtta
    17221 ggcggtgaca atgggttaca ttatgatcaa attattaata atatcaccac cagcccattg
    17281 agttatttcc tggcgttcat tggggcgttt atctgggcag cctactgcac agtaacgaat
    17341 aaatacgcac acggatttaa cggaattacc gtttttgtcc tgctaacggg agcaagtctg
    17401 tgggtttact attttcttac gccacaacca gaaatggtat ttagcacgcc cgtcatgatt
    17461 aaactcatct ctgcggcatt taccttagga tttgcttatg ctgcatggaa tgtcggtata
    17521 ttacatggca acgtcaccat aatggcggta ggttcgtatt ttacgcccgt cctttcctca
    17581 gcgcttgcag ccgtgctgct cagcgccccg ctgtcgttct cgttctggca aggcgcgcta
    17641 atggtctgcg gcggttccct gctctgctgg ctggcgacac gtcgtggcta aaataattgt
    17701 cgggtctacg gcccggcagt taatcagata aattctgaca atattaa
//
LOCUS       DAZFFE010000099        17054 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFFE010000099 DAZFFE010000000
VERSION     DAZFFE010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.099"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(70..1092)
                     /locus_tag="ACHJ69_003662"
     CDS             complement(70..1092)
                     /locus_tag="ACHJ69_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFO5016558.1"
                     /translation="MSTMKVLICQEIKNLVCEVRERPVPGDNEALIKIKTVGICGTDI
                     HAWAGNQPFFSYPRVLGHEICGEIIESGKNIHNFNIGQQVAVIPYISCQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APSEQVLVVGAGPIGLGAAAIAKADGAQVVVVDTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            1231..2136
                     /locus_tag="ACHJ69_003663"
     CDS             1231..2136
                     /locus_tag="ACHJ69_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO5016559.1"
                     /translation="MSRSQNLRHSVINQVIEDMARGNIPSPLPSQSGLAEMYNISRTT
                     VRHILQHLSACGVLTLVGKNYVIARNPEQNDGFDCITASLKEQNRLFEQAFFTMINLR
                     QLRAGESFSELQLARAASVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDDDPRWLQAKTMLERHRTLRDSIGSNFRMFSQLDRDFHA
                     MLLSAADNIFFNQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     AITALRNHLDTAKQSMIRSIRQHHR"
     gene            complement(2307..3959)
                     /locus_tag="ACHJ69_003664"
     CDS             complement(2307..3959)
                     /locus_tag="ACHJ69_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO5016560.1"
                     /translation="MEILLTILILTLVVSLTSVLTRLSPVQIPLPLIQIAAGAVLAQP
                     IFGLHVEFNPELFLLLFIPPLLFAESSKIQPKELIKHSREIISLALVLVLITIFGVGY
                     VIHLLLPNVPLIAAFALAAVLSPTDAVALLGIVGKGRISKNIQEVLEGEALMNDASGL
                     VALKFAVAVTMGTMEFSVHGATIAFFVVALGGIAVGIAVTWLYGKGLLLISRYAHDEP
                     SIQIVLMLLLPFIVYLVAEHFGLSGILAAVAAGLTTSRIGVVHHAPIRLRIKAKSSWS
                     MLEYVFNGMVFLILGLQLPGIISESLIRAENDPLVDTGSLLLDVIWIYLALMVVRLGW
                     LWLMRLYSRKRPGKHPMLFAGYKTRHMLLATFAGVRGAITLAGVLSIPLLLPDGTPFP
                     GRYLLVFLATGVILFSLVCGIIILPLLLGKNVGIFSDNRQKEEEAADLFMAKMAILSV
                     QKTEQRLLRARTENLDNELIMETGARVIGYMRHSEEDMSPNDNSLHMQRAKLERTFNL
                     AALNAKRAAIYQMRARQEVSDETLAKIVSELDVLETLIMDSR"
     gene            4650..5621
                     /locus_tag="ACHJ69_003665"
     CDS             4650..5621
                     /locus_tag="ACHJ69_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HFO5016561.1"
                     /translation="MSKIIAMLITVSTLFLPSIIQAKPISIKVAYENNPGEPLDVVMR
                     YWADLLNKKSNGEITLALYPSSQLGSKQDVTEQAMMGMNVITLSDVAFLADYEPDLGI
                     LFGPYLTDDPQKLFKIYESDWFKQKNESLKKKGIHVVMNNYLYGTRQIISKKPIRTVD
                     DLAGLKIRVPNNVMQIKAIQAMGATPTPMPLGEVYPALTQGVIDGVENPISVLQGQKL
                     YEQARYLTMVNYLTNTSVWIGGEAFFSTLSPEQLEMIHQTGYEAGVYSQKLTLERDAE
                     MLKTMQAAGVEVIYPDTGPFQKKAREVYSQFPEWTPGLYETIQQQLQ"
     gene            5685..6158
                     /locus_tag="ACHJ69_003666"
     CDS             5685..6158
                     /locus_tag="ACHJ69_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO5016562.1"
                     /translation="MRLFGKVAEFSAAFALFVLVVVTIGAVFMRYFIGQPLQWTEEMS
                     GMLMIWVVMLGGVVAERDRAHLTIPFLMEMLPGKLRRVIAVLVALLSIALLLYMAWLG
                     YRLAEMAQFKVTQILKVSWFWIDLAVPVGALATAIYTLYWLINDTKQSDSGDKMQ"
     gene            6155..7426
                     /locus_tag="ACHJ69_003667"
     CDS             6155..7426
                     /locus_tag="ACHJ69_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO5016563.1"
                     /translation="MSWLLIIPLMIVCLFLNIRVYLAMFIGIIAYFLFFSSVPIEIAV
                     QRLIAPTQSPSLLAIPFFILLGTLMSYTGIAERILHIANVLVGKMRGGLGVANILVST
                     MMGGVSASNLADAAMLSRMMVPEMERKGYNRAFAAAITAGGSLVTPIIPPGIALIIYG
                     LVADVSIGKMFIAGLVPGLLCAVVLMFTVYLVARKTNAKPSRESWPTGKEVVFSLGQA
                     WPALFLIFAVVGGIRANIFTPTEAGAVAVLIVLAIGFFIYREMRISHVVKALGETARA
                     TASVMLVIMASAALGWIFSMEHAGVAVANFVTSLTENKYMFLLIINILLLTLGMFLEG
                     NAILLILVPLLKPVVEHFGIDPVHFGIIMIFNLSIGAFTPPVGTVMLLVCNITQVSVG
                     NFFKQSLPLLAALLLMLMLVTYIPAISLFLV"
     gene            complement(7469..9151)
                     /gene="tsr"
                     /locus_tag="ACHJ69_003668"
     CDS             complement(7469..9151)
                     /gene="tsr"
                     /locus_tag="ACHJ69_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO5016564.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVILAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQQQQQQQRETSAVVKTVTPATPRKMAMADSEENWETF"
     gene            9528..11678
                     /gene="btsT"
                     /locus_tag="ACHJ69_003669"
     CDS             9528..11678
                     /gene="btsT"
                     /locus_tag="ACHJ69_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO5016565.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVIPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLKVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            11796..11999
                     /locus_tag="ACHJ69_003670"
     CDS             11796..11999
                     /locus_tag="ACHJ69_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO5016566.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            12010..12966
                     /gene="yjiA"
                     /locus_tag="ACHJ69_003671"
     CDS             12010..12966
                     /gene="yjiA"
                     /locus_tag="ACHJ69_003671"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO5016567.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEVLCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLRK"
     gene            complement(13000..13302)
                     /locus_tag="ACHJ69_003672"
     CDS             complement(13000..13302)
                     /locus_tag="ACHJ69_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HFO5016568.1"
                     /translation="MSMVKHKRGTSSTLDVQHEAELKALANKFDEDIDYSDIPSSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(13289..13576)
                     /locus_tag="ACHJ69_003673"
     CDS             complement(13289..13576)
                     /locus_tag="ACHJ69_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HFO5016569.1"
                     /translation="MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIEN
                     GEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIISARKADRKERNRYEHG"
     gene            13773..14939
                     /locus_tag="ACHJ69_003674"
     CDS             13773..14939
                     /locus_tag="ACHJ69_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO5016570.1"
                     /translation="MKNYKVITPLFPTYAQVKAMMKAVSGYSLKAVRNMITAIHEQTG
                     TPQKPVDWSEPDLWISERLTGEDADIARRIWDTDNHILNPRHSYGCYLFLNYPQFELM
                     ESTPDDIWQPTSRGQKFLQDDEKTLRSLDDQEGILQLLELLAGREMSRRADLLPEWQA
                     FLHQHSKFASASSVKSTLYSRLYNLIDRDMVNREGMSYRITDTGRAWLGKALPARKSD
                     PRKELLEAVKRYNEQQKELLREQISTMNPYKFEQLVGQLLEAMGYEQVEVTKASGDKG
                     VDVIGQVQVGITTITEVVQVKRMQNTITRPYIDQLRGALPYHKAIRGTLITTGKFAAK
                     CAEAALFPGAAPITLIDGDRLLELLIENNVGIRRSNAVELLDVDLQLFDELEMD"
     gene            15003..16622
                     /locus_tag="ACHJ69_003675"
     CDS             15003..16622
                     /locus_tag="ACHJ69_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO5016571.1"
                     /translation="MAKAPTKKAKAKKGFEETLWDTANQLRGSVESSEYKHVVLSLVF
                     LKFISDKFEARRKKMIADGQADFLEMEVFYQQDNIFYLPEEARWSFIKQNTKQDDIAV
                     RIDTALSTIEKRNPTLKGALPDNYFSRQNLETKKLASLIDTIDNIETLAHETDVETLS
                     KEDLVGRVYEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGKIYDPCCGSAGM
                     FVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLSANLGERPADTFFSDQ
                     HPDLKADYILANPPFNLKDWRNEAELTKDPRFAGYRMPPTGNANYGWILHMLSKLSAN
                     GTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFYTTQIPVCLWFMTKS
                     KAADPAKGYRDRQGETLFIDARNLGTMISRTTKELTTEDIATIADTYHAWRSTPEELA
                     ARIARGDSKLEKYEDQAGFCKVATLQDIKDNDYVLTPGRYVGAAEQEEDGVAFETKMR
                     ELSKTLFEQMKQAEELDRAIRKNLEALGYGL"
     gene            16612..>17054
                     /locus_tag="ACHJ69_003676"
     CDS             16612..>17054
                     /locus_tag="ACHJ69_003676"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO5016572.1"
                     /translation="MGFDFQLLENAGIQLIDGDRGKNYPKHNDFMENGYCLFLSAKNV
                     TKSGFQFQETLFINETKDRELRAGKLKYGDIVLTTRGTVGNVAYYDNNNPYKHIRINS
                     GMIIIRADNKLWNPKFLYFILKSELLKEQIINLISGSAVPQLPAR"
BASE COUNT         4380 a         4366 c         4034 g         4274 t
ORIGIN      
        1 gaaacagaaa aaaaacagcg cccaacactt ccccggacgc tgcatggcaa cttgaataat
       61 ctcgccttat cagaaagtaa ttacgccctt aatcaactca cgattgttaa tcacatcgcg
      121 ctcgtaggtt tctgccagcg tggcgaacgg atagcgatgg gttaacatca tgtcggcagt
      181 gattttccct tccgccatca atcgaccgac tttagcgaaa tcttccggcg tggcgttgcg
      241 gctgcccatc atcgtcgttt cttttttatg gaattccgga tcggagaact gcaactcacc
      301 tttaaacagg ccgacaaata ccaccgtgcc gccgtgacga atcagattta cggtgttatt
      361 catcgcatgt tgattacctg tcgcgtcaat cactttctgc gccagcgaac caccaaactg
      421 cgcccgcagc tgcgcgtcaa aatcttcggc tgacgggtcc agaacaggta attccagacg
      481 cgttgccaca tgttcacgac gcgccggact ggtatccacc accaccacct gcgcgccatc
      541 ggctttagcg attgccgccg cgcccagacc gattggcccc gccccgacca ccagcacctg
      601 ctcgctgggt gcaatggctg cacgacgcac cgcatgagcg ctaatagcga aaggttcaat
      661 caatgctgcc gcctgcgggt caataccgtc tgcgggcaaa atgttcgcca ccggcacgct
      721 caaatactca ctaaaaccgc catcctgatg cacgccaatg actgaaattt tttcacagca
      781 attggtacga ccgcttttac acgccgggca ttgctgacaa gaaatatacg gaattaccgc
      841 aacttgctgt ccgatattaa aattgtgaat gttttttcct gattcaataa tctccccaca
      901 tatttcatgg cctaaaacgc gtggataact aaaaaatggc tgattgcccg cccaggcatg
      961 aatatctgta ccgcaaatcc cgacagtttt aattttaatt aatgcttcat tatcgcctgg
     1021 caccggtctt tcccggactt cacatactaa attctttatt tcctgacaaa ttaatacttt
     1081 cattgtggac atgattcacc gctcctttta ttttatgact ttcagatatc gtcgaattta
     1141 taagagcgcc tgaatttacc cataaaatat gcggatgatc gcgcataaaa atgttttaaa
     1201 tcggctttta atgccggact ggagggaaga atgagtcgtt cgcagaattt acgtcatagc
     1261 gtcataaatc aggtgattga agatatggcg aggggcaata ttccttctcc gctgccttca
     1321 caaagcggac tggcagaaat gtataacatc agtcgcacta cggtacgcca catactccag
     1381 catttaagcg catgtggcgt gctcaccctg gtggggaaaa attatgttat cgcccgcaac
     1441 cccgaacaga acgatggatt tgattgcatc acggcctcgc tgaaagagca aaaccgtctt
     1501 ttcgagcagg catttttcac catgattaac ctgcgccagt tacgcgccgg agaaagcttt
     1561 tccgaacttc agctggcgcg agcggcaagc gtcagtccgg tagtggtacg agaatatctt
     1621 ttgaaattcg gtcgttataa tcttattcaa agcgaaaagc gcggccaatg gagcatgaag
     1681 cagttcgatc aatcctacgc cgagcagctg tttgagctac gcgaaatgct cgaaacacat
     1741 tccctgcaac actttctcaa ccttcccgat gatgaccctc gctggctgca agcgaaaacc
     1801 atgctcgaac gccaccggac gctgcgcgac agcattggca gcaactttcg aatgttttct
     1861 caattagacc gtgattttca tgccatgctg ttatccgctg ctgacaacat cttttttaat
     1921 caatcacttg aaattatatc ggtgatcttt cattttcact atcaatggga tgaaagcgat
     1981 ctcaaacagc gcaatatcat tgctgttgat gaacatatga ccattctcag cgccctgatt
     2041 tgccgtagcg atttagatgc aataacggcg ttgcgaaacc atctggatac cgccaaacaa
     2101 tcaatgattc gttccatcag gcaacatcac cgataaatta agtacagatt aaaaacattg
     2161 attcttggtt tatatcacta tgttgtatta atgcagtatt aatccaggcg attgttctgc
     2221 ccataaggaa atacataggg aataaacgat gctgaaagta ttactctcag ccatcattta
     2281 attccctatt actggcagcg ataaaattat cggctatcca tgattaacgt ttccagcaca
     2341 tctaattcac tgactatttt tgccagtgtt tcatcactga cttcttgcct tgcgcgcatc
     2401 tggtaaatag ccgcacgttt ggcatttaac gccgccagat taaatgttct ttccagcttc
     2461 gcacgctgca tatgcaggga attatcatta ggcgacatat cctcttcact atgtcgcata
     2521 taaccgataa ctctggcccc cgtttccata atcaactcgt tatcgaggtt ttctgttctg
     2581 gcgcgcagca aacgttgctc ggtcttttgc acactcagta tggccatttt agccataaac
     2641 agatcggcag cttcctcttc tttttgtcgg ttatcgctga aaatccccac atttttccct
     2701 agtaataacg gcagaataat aatgccgcaa accagcgaaa agaggatcac acctgtagcg
     2761 agaaatacca gcaagtaacg cccgggaaaa ggtgtgccat ccggcagcaa taacgggata
     2821 gaaagcaccc ccgcaagcgt gatagctccg cgcacccccg caaaggtcgc cagcagcata
     2881 tgacgcgttt tatatccagc aaacagcatc ggatgtttgc cgggtcgttt acggctatac
     2941 agacgcatca gccaaagcca gcccaggcgc acaaccatta acgccaggta gatccaaatg
     3001 acatcaagga gcagcgatcc ggtgtccacc agtggatcgt tttctgcacg gatcagtgat
     3061 tccgaaataa tgcccggcag ttgtaagcct aaaatcagaa acaccatgcc gttaaagaca
     3121 tattcaagca tcgaccagct acttttagct ttaattctca ggcgaatagg cgcgtggtgg
     3181 actacgccaa tgcgactggt tgttaacccc gcggcgacag cggcaagaat gccagataag
     3241 ccaaaatgtt ccgcaaccag ataaacaata aacggcaaca gaagcatcaa tacaatctga
     3301 atggaaggtt catcgtgagc atatcggctg atcaacagca gccctttacc atataaccag
     3361 gtaacagcga tccccacggc aataccgccc aacgcaacga caaaaaacgc aatggttgcg
     3421 ccatgaacgc tgaattccat cgttcccatc gtcactgcaa cagcaaattt caacgccacc
     3481 aggccggaag catcattcat caacgcttcg ccttcgagca cttcctggat gtttttgctg
     3541 atacgccctt tcccgacaat ccccagcaac gccacggcat cggtaggcga caacacggcg
     3601 gctaatgcga aagcagcgat gagtggcaca ttcggtaaga gtaaatgaat cacatagccg
     3661 acaccaaaaa tagtgattag taccaacaca agtgccaggc tgataatttc ccggctgtgc
     3721 ttgatcaatt ctttaggttg tatttttgaa ctttcagcaa acaacagggg tggtataaac
     3781 aataagagaa aaagttcagg attgaattcc acatgcagac caaatatagg ctgggccagt
     3841 actgcaccag cggcaatctg gattaaagga agtggtatct ggacaggaga cagacgtgta
     3901 agcacgctgg ttaatgatac taccaacgta agaattaaaa ttgttaaaag tatttccata
     3961 taataaacag gcttcctcat tcataaataa tggatattat taatttaact tcataaaaag
     4021 cgctctggtt agcacttaac gttaatcatt aatcactgcg ttcactcaca tgatcagaag
     4081 gaacccagta aaataagttt tcaagttgat aacaaattca ccatgtgaat atttactgcc
     4141 tttccccgtt gtaacgaatc atccgttact ctctgatttc cttgccgaac aagttattta
     4201 gtgaatgacg gctttctttt aatatcagca tgaattatac gttcagcaaa tgtattaaat
     4261 cagcactaaa taaatttaat ggataccacc attctgcgtc aacacttagt ttttatctgt
     4321 attgacaatt aaaaatgacc gcagccaaat taatgatttt atttttaata aatcatcaac
     4381 cgaataagag atcttatcag cggtaactaa actcatcaga gggtgttcct tgtaacgtca
     4441 tgcattacat aactttaaga tatacaatca aaaaatgcat taaaaaccga ttaaaagcag
     4501 caaagtatta cccggtgcac gaatttatga tacatctgca gccattctgc tattcgctga
     4561 ctacttttca gtaatgactg ctttttcata aggtttcact cccgacaaca atgttggagt
     4621 tttatttcct tagcatggag taagtaaaga tgagcaaaat aatagcgatg ctaattactg
     4681 tcagtaccct ttttttaccg tcaataatcc aggcaaaacc tatttcaata aaagttgcct
     4741 atgaaaataa ccctggggaa cccctggatg tggtcatgcg ttactgggca gacctattaa
     4801 ataaaaaaag taatggcgaa ataacacttg cgctctatcc cagctcccaa ttgggatcaa
     4861 aacaggatgt caccgagcaa gccatgatgg gcatgaatgt cataaccttg agtgatgtgg
     4921 cttttcttgc cgattatgaa cccgatttgg ggattttgtt tggaccgtat ctgactgacg
     4981 atccgcaaaa actgttcaaa atatatgaaa gcgactggtt taaacaaaaa aatgaaagtc
     5041 tgaagaaaaa gggaattcat gtggtaatga ataattatct ctatggcact cgacagatta
     5101 tttcgaaaaa acccattcgc accgttgacg atctcgcagg tttaaaaatt cgcgttccta
     5161 ataacgtcat gcaaataaaa gccattcagg cgatgggggc aacaccaact cccatgccat
     5221 taggcgaagt ttatcccgca ttaacccagg gcgttatcga cggtgtagaa aacccaattt
     5281 cagtgctgca agggcaaaaa ctgtatgagc aggcaagata tctgacaatg gttaactacc
     5341 tgacaaatac ctcagtgtgg ataggtggtg aagccttctt tagcaccctt tcaccagaac
     5401 aactggagat gatccaccag accggctatg aagcgggtgt ctatagccag aaactcactc
     5461 ttgagcgtga tgccgaaatg ttgaaaacca tgcaggcagc cggcgtcgaa gtgatctatc
     5521 ccgatactgg accattccag aagaaagcac gcgaagttta ctctcaattc ccggaatgga
     5581 caccaggtct gtacgaaacc attcaacagc aactgcaata agcattaatg ctcagcgtcc
     5641 tgcctcatct ctcttaggca ggacacgcac taacaggagg tttcatgcgt ctttttggca
     5701 aagtggcaga atttagtgct gcctttgcgc tattcgttct ggttgtggtg acaattggag
     5761 cggtatttat gcgttatttc atcggccaac cgcttcaatg gacagaagaa atgtcgggaa
     5821 tgctgatgat ttgggtggtg atgttaggtg gagtggtggc ggaacgtgac agggcgcacc
     5881 tgaccattcc cttcctgatg gagatgcttc caggcaaact caggcgtgtt attgcagtac
     5941 tggtcgccct tctctccatc gcactgctgc tttatatggc ctggctgggc taccgactag
     6001 ccgaaatggc gcagtttaaa gtgacacaaa ttctgaaggt ttcctggttc tggatcgatc
     6061 tggcggtccc tgtcggcgca ctggcaacgg ctatttatac cctctactgg ttaataaacg
     6121 atacaaaaca atccgatagc ggagacaaaa tgcaatgagt tggttattga ttattccgct
     6181 aatgatcgtc tgtttgtttc tcaacattcg ggtctatctg gcgatgttta ttggcatcat
     6241 cgcttacttt ttattcttct ccagcgtacc gattgaaatt gccgtgcaaa gactaatcgc
     6301 gccaacgcaa agcccatcat tgctggcaat tccgtttttt atcttgctag gcacactaat
     6361 gagctacacc ggcattgccg aacgcatcct gcacattgcc aacgttctgg tggggaaaat
     6421 gcgcggcgga ctgggcgtgg caaacattct cgtcagcacc atgatgggcg gcgtcagcgc
     6481 ctctaacctt gctgacgcgg cgatgctgtc gcgcatgatg gtgccggaga tggagcgtaa
     6541 aggctacaat cgcgcgtttg ccgccgccat taccgctggt ggctcgttag tcaccccgat
     6601 cataccgcct ggaatagccc tgattattta cggtctggtt gccgacgtct ctatcggaaa
     6661 aatgtttatc gccggactgg tacctggttt gctatgcgca gtcgtactga tgtttaccgt
     6721 ctaccttgtc gcccgcaaaa ctaacgctaa accctcgcgt gaaagctggc ctacgggcaa
     6781 agaggtggtg ttctcgctgg gacaggcctg gcctgcgctg ttcctcattt ttgctgtggt
     6841 gggtggtatt cgcgctaaca ttttcacccc aacagaagca ggtgcagtag cggtgctaat
     6901 cgtgctggcg attggctttt ttatctatcg tgaaatgcgg atttctcatg tcgtcaaagc
     6961 attgggcgaa acggcacgcg caaccgcatc ggtgatgctg gtaatcatgg ccagcgccgc
     7021 attgggatgg attttctcaa tggaacatgc tggtgtcgca gtggcgaatt ttgtgacttc
     7081 gctgaccgaa aacaaataca tgttcctgtt gattatcaac atattgctgc tgacgctggg
     7141 gatgtttctg gaaggaaacg ccattttgct gatcctggta ccactattaa aaccggtggt
     7201 cgaacacttt ggcatcgatc cggtgcattt tggcatcatc atgatcttca acctgtcgat
     7261 tggcgcattc acgccaccgg taggtaccgt gatgctgctg gtatgtaata tcacgcaagt
     7321 ttcggtggga aatttcttta aacaatccct gcccctactg gcggctctac tgttaatgct
     7381 aatgctggtg acatatatcc cggcgatttc actgttcctg gtttaataat aaaaacgccg
     7441 gatgaaatac tcatccggca tcattacgtt aaaacgtttc ccagttctcc tcgctatctg
     7501 ccatggccat tttacgcggc gtagctggtg tcacggtttt taccacagcc gatgtttcac
     7561 gctgctgttg ctgctgttgc tgctgttgct gctgttgctg aatccggaac actgccactg
     7621 cttcggtcag gcgactggcc tgctcttcca gcgcggcggc ggcggcggca gattcttcca
     7681 ccagcgcggc gttttgttgg gttacccggt ccatctcagc aaccgctaag ccaacctgat
     7741 cgataccacg gctctgctca tcagaagcag aagcaatttc gcccataatg tccgtcacgc
     7801 gggtcacggc actgacaatc tccgccattg tttccccagc gctttcgacc agcgtagagc
     7861 caacatccac tttccccacc gagtcttcaa tcaggctttt aatttcacga gcagcctgag
     7921 cgctacgctg ggccagatta cgcacttctc ccgcgaccac cgcaaaaccg cgtccttgct
     7981 cacccgcacg cgcagcctca accgccgcgt tcaaagccag aatattggtc tggaaggcaa
     8041 tgccgtcaat tacgctgata atatcggcga ttttctgcga actggtggag atatcgcgca
     8101 tggtctgcac cacgttatcc accactttgc cgccgcgttg cgccgtttcg gaagcactta
     8161 acgccagatg gctggcctgg cgcgcatttt cagcattctg tttcaccgtt gcggtcagtt
     8221 gctccatgct ggctgccgtc tcttccagcg aagcggcctg ttgctcggtg cgcgaagaga
     8281 gatcgttatt gccggtggcg atttcgctgg caccgctata gatggcattg gccccgttgc
     8341 gcacatcacc gacggtacgc atcagctctc cctgcatatg gcgcaaactc tctgccagtt
     8401 gccccatctc attagagcca tccacctcaa tcggtttcac cagatcgccg cctgcaatat
     8461 gacgaatgct gtcaatcagg cgattcattg gcgctaccag cgaggcttta ataccgaacc
     8521 agacggcgag gatgaccgcc agtacgacga tcatcacgcc taccagaatc cacatcgcct
     8581 ggctgtagga ggcattgtta tcgctgacgg cgatatcata gagccgatcg ttttgctcca
     8641 tgtaagccac atactgcttc tcgaaaccgt cctgatatcc ctgggtcggc tgatcaaaga
     8701 actcgttgat tttgcctgca cctaacagtt gtatcagctc cgccagcgca ttgtgataaa
     8761 tatcgtaatt acgtttgatc tctgccgctg cggcggtgct ctgacgcggg tcacgcggca
     8821 acgcttcgta atccgcccag tttttttccg cctgtttcag cgaaatactg gcactctgca
     8881 tcagctcagc aacggttgaa ccgctaccaa tattgttctg atccatcatg tagcggatac
     8941 ccgcgcggtt gagggtgtta cgcgtctgca acaacgcgac ccagctgcca ttcagcgtgg
     9001 attgctgctg gcgaatggtt tgtaaaacag tgaaattttc tttgtcgttc tttaaggcat
     9061 taaagaacag accgcctgaa gtcagttgta aaaggccaaa aacggccaaa accagcagta
     9121 agctggtcac aattttgata cgttttaaca tggtttctct ttcctgtgga ccgatgcaag
     9181 attttcggcc tggaaaggaa aaactttatg aaaatcccgc ggagatgaca tcgagtaatt
     9241 ttattcaccc cctgaattta aaaagccatt atttacataa attattcgca atataaatat
     9301 gtgatataaa tcacatattt atggtcactt aaacgacgcc tttgccgctc accgccattc
     9361 ctgaccgctt acgtccctaa aataaccact cagttattta ccttacttta cgcgcgcgta
     9421 actctggcaa catcactgca ggatagcggt caatttacct cctcaaacgc aacgcaaacc
     9481 tagaacggct tcggccaact attaatcaat acatgccagg ttttactatg gatactaaaa
     9541 agatattcaa gcacataccc tgggtgattc tcggaatcat cggtgcattc tgcctcgcgg
     9601 tagttgcatt acgtcggggg gagcacgtca gcgccctgtg gatcgtggtc gcctctgtgt
     9661 cggtgtatct ggtggcgtat cgctactaca gtctgtacat cgcccagaag gtgatgaaac
     9721 tcgaccccac gcgcgcgacg cctgcggtta ttaacaacga cggtctgaac tacgttccga
     9781 ccaaccgtta cgtgttgttt ggtcaccact tcgccgctat cgccggtgct ggtccgctgg
     9841 tgggtccggt tctcgccgcg cagatgggct acctgcctgg cacgctgtgg ctgctggcgg
     9901 gggtcgtgct ggccggtgcg gttcaggact ttatggtgct gtttatctcc tctcgccgta
     9961 acggcgcatc tctcggtgag atgatcaaag aagagatggg accagtaccg gggactatcg
    10021 cgctgtttgg ctgtttctta atcatgatca tcatcctcgc cgttctggcg ctgattgtgg
    10081 ttaaagccct ggccgaaagt ccgtgggggg tcttcaccgt ttgctcaacc gtaccgattg
    10141 cgctgtttat gggtatctac atgcgcttta ttcgtccggg gcgtgtgggt gaagtctctg
    10201 tcattggtat cgtgctgctg gttgcctcta tctacttcgg tggcgtgatt gctcacgatc
    10261 cgtactgggg tccggcactg acctttaaag acaccaccat taccttcgcg ctgattggct
    10321 atgcgtttgt ttccgcactg ctgccagtgt ggctgatcct cgcaccgcgc gactatctgg
    10381 caaccttcct gaaaatcggc gttatcgtcg gcctggcgct gggtatcgtg gtgctgaacc
    10441 cggaactgaa aatgcctgcc atgacccagt acattgacgg tactggcccg ctgtggaaag
    10501 gcgctctgtt cccgttcctg ttcatcacca tcgcctgtgg tgcggtatct ggcttccacg
    10561 cgctgatctc ttccggtacg acgccgaaac tgctggctaa tgaaaccgac gcgcgtttca
    10621 tcggctacgg cgcaatgctg atggagtcct tcgtggcgat tatggcgctg gttgctgcgt
    10681 ccatcatcga accgggtctt tacttcgcga tgaacacccc gcctgctggc cttggcatca
    10741 ccatgcctaa cctgcatgaa atgggtggcg agaacgcgcc gatcatcatg gcacagctga
    10801 aagacgttac cgcacacgcg gcagcgaccg tcagctcctg gggcttcgtg atttcgccag
    10861 agcagatcct gcaaaccgcg aaagacattg gtgagccttc tgtcctgaac cgtgcaggtg
    10921 gtgcgccaac gctggcggta ggtatcgctc acgtgttcca caaagtgctg ccgatggctg
    10981 acatgggctt ctggtatcac ttcggtattc tgttcgaagc cctgttcatc ctgaccgcgc
    11041 tggatgcggg tacccgttct ggccgcttta tgctgcaaga cctgctgggt aacttcatcc
    11101 cgttcctgaa aaaaaccgac tctctggttg ccggtatcat cggtactgcg ggctgtgtgg
    11161 gtctgtgggg ctacctgctg tatcagggcg tggtcgatcc gctgggcggc gttaagagcc
    11221 tgtggccgct gttcggtatc tccaaccaga tgctggcagc cgtggcactg gtactgggca
    11281 ccgttgtact gattaagatg aagcgcaccc aatacatctg ggttaccgtt atcccggcag
    11341 tatggctgct tatctgcacc acctgggcgc tgggcctgaa actgttcagc accaacccgc
    11401 agatggaagg cttcttctac atggcaagcc agtacaaaga gaagattgct aacggtactg
    11461 acctgacggc gcagcagatt gccaacatga accacatcgt tgtgaacaac tacaccaacg
    11521 caggcctgag cattctgttc ctgattgtgg tgtacagcat catcttctac ggtttcaaaa
    11581 cctggctgaa ggtgcgtaac agcgacaaac gtactgacaa agaaacaccg tacgttccaa
    11641 tcccggaagg cggcgtgaag atctcttcgc accactaacc ggttgcctta tccggcttac
    11701 aaagtgtcta tcgtaggccg gataaggcgt ttacgccgca tccggcaaat ccctagcccg
    11761 gaccttccgg gctttacgca tatctggaag gcaaaatgtt tggtaactta ggacaggcaa
    11821 aaaaatatct cggccaggcg gcgaagatgt tgattggcat tccagactac gacaactacg
    11881 tcgagcatat gaagaccaac catcccgaca agccgtacat gagctatgaa gaattcttcc
    11941 gcgaacgcca gaacgcgcgc tacggcggcg atggtaaagg cggtatgcgc tgttgttaat
    12001 ggagagagca tgaatccgat tgcagttacc ctactcaccg gttttttagg cgcaggaaaa
    12061 accaccctgc tgcgccatat tcttaacgaa caacacggct acaagattgc cgtgattgaa
    12121 aacgaattcg gcgaagtctc cgttgatgat caactgattg gcgatcgcgc cacgcagatc
    12181 aaaacgctga ccaacggctg catctgttgt tcgcgctcca acgagctgga ggacgcgcta
    12241 ctcgacctgc tggacaatct cgacaagggc aatattcagt tcgatcgtct ggttattgaa
    12301 tgcaccggca tggccgatcc cggcccgatt attcagacct ttttctccca tgaagtttta
    12361 tgccagcgtt acctgctgga cggagtgatt gcgctggtgg atgcggtaca tgccgatgag
    12421 cagatgaacc agttcaccat cgcccagtca caggttggct acgccgaccg tattctgctg
    12481 actaaaaccg acgtcgcagg cgaagcagaa aaactgcgtg aacgcctggc gcgcatcaac
    12541 gcccgcgcac cggtctacac cgtcacccac ggcgacatcg acctgggcct gctgttcaac
    12601 accaacggtt ttatgctgga agaaaacgtc gtcagcacca agccacgttt tcactttatc
    12661 gcggacaaac aaaacgatat ttcgtcgatt gtggtggaac tggattaccc ggtagatatc
    12721 agcgaagttt cccgcgtgat ggaaaacctg ctgctggagt cggcggataa actactgcgt
    12781 tacaaaggga tgctatggat tgacggcgaa cctaaccgcc tgctgttcca gggcgtccaa
    12841 cgcctctaca gcgccgactg ggaccgcccg tggggcgatg aaaaaccgca tagtacgatg
    12901 gtgtttatcg ggattcagtt gccggaagat gagattcggg cagcgtttgc ggggttaagg
    12961 aagtaatttg ggatgatggc cgccatgtgg cggcctgaat tattttttgt tctgatcgcg
    13021 cagcatcgct tcacgtaaaa ttgcgttcag gcgagtttga tagcctttac caggtcgctt
    13081 caaccattcc atcacatcag catcaatacg caccgaagcc tgagttttca atggccgata
    13141 aaacttaccg cgttcggcgt tagaccattg ctcatcgctg gaagatggga tgtcgctgta
    13201 atcaatatcc tcgtcgaatt tgttcgccag cgccttcagt tcggcctcat gctgcacatc
    13261 gagtgtagag gaggtccctc gtttatgttt aaccatgctc atagcgattc ctctctttac
    13321 gatctgcttt tcgtgcactg atgattcgaa taatttcatt tccgctttcg aaacggatgg
    13381 tatgcgcaac cagaataacc acgatgccgt aaaccaggcc gattgtctgc cagcgatact
    13441 caccgttttc tatgcgatct tgctgtgaca aatgttgtgg atcatcaaac actaacaccg
    13501 catcttcaaa gcggatgcca tgttttaccc gattactttt ggccttgttt gcatcccatt
    13561 caaactccat tggcatagtt gccatcccta attgtatata caaaaatgta tctacaatta
    13621 ttctgcaatt atagcgttga tgttggaaat agcatcacgt tttggataat caaccatgaa
    13681 aatgacaatg agtgagttgg acttgattca tccaatagaa tttctcgcct gacatggcac
    13741 actaaacacc tcgctgtcgt taagggatta ccgtgaaaaa ctacaaagtc attaccccgc
    13801 tttttcctac ctatgcgcaa gttaaggcta tgatgaaagc cgtctcaggc tattccctga
    13861 aagcggtacg caatatgatt accgccattc acgagcaaac cggtacgccg caaaaacccg
    13921 ttgactggtc agaacccgac ctgtggatca gcgagcgctt aacgggtgaa gatgccgaca
    13981 tcgcccgtcg tatttgggat accgataatc acatcttgaa cccgcgccac agctacggct
    14041 gctacttatt cctcaactat ccccagtttg agctgatgga aagcacacca gatgatatct
    14101 ggcagcccac ctcgcgcggg caaaaattcc tgcaagatga cgaaaaaacg ctgcgttcac
    14161 tggacgacca ggaaggaatt ctgcaactgc ttgagctgct ggctggccgg gaaatgtcgc
    14221 gccgcgccga tttactgccg gaatggcagg cctttttgca tcagcattcg aaattcgcgt
    14281 ctgcaagttc agtgaaaagc acgctctact cccgtctcta taaccttatc gaccgtgaca
    14341 tggttaaccg tgaaggcatg agctatcgca tcaccgatac cgggcgcgcc tggcttggaa
    14401 aagcgttgcc tgcacgaaaa agcgatccgc gtaaagagct gctggaagcc gtgaagcgct
    14461 acaacgagca gcaaaaagag ctgctgcgcg aacaaatcag cacgatgaac ccgtacaaat
    14521 ttgaacagct ggtcggtcaa ttactggaag caatgggcta cgagcaggtc gaagtgacca
    14581 aagcctctgg cgataaaggc gtggacgtga ttggtcaggt gcaggtcggc atcaccacca
    14641 taaccgaggt ggtgcaggtc aaaagaatgc agaacaccat cacccgccct tatatcgacc
    14701 agttgcgcgg ggcattgcct taccacaaag ccattcgcgg tacactcatc accaccggga
    14761 aatttgcggc gaaatgtgca gaagcggcgc tctttccggg tgccgcgccc attacgctta
    14821 tcgacggcga tcgtttactg gaactgttaa tcgaaaataa cgtcggcatt cgccgcagca
    14881 atgcagttga attgctggat gtagatttgc agttgtttga tgagctggag atggattaaa
    14941 gggcgaaatc cgccgcctca accattacaa tagcgaccca atttatctga acagagttca
    15001 tcatggccaa agcaccaact aaaaaagcca aagcaaagaa aggctttgaa gaaacattat
    15061 gggatacagc aaaccagctt cgcggtagcg ttgagtcctc cgaatacaag cacgtggtgt
    15121 tgagcctcgt gttcctgaaa ttcatcagcg ataagtttga agcacgccgc aaaaaaatga
    15181 ttgccgatgg gcaagcagac ttccttgaga tggaagtgtt ctaccagcag gacaacattt
    15241 tctacctgcc ggaagaggcg cgctggtcat ttatcaaaca aaataccaaa caggacgata
    15301 ttgcggttcg tattgacacc gccctctcga ccattgagaa acgtaaccca accctgaaag
    15361 gcgcgctgcc agacaactac ttcagccgtc agaatctgga aaccaaaaaa ctggcgtcac
    15421 tgattgatac catcgacaac atcgaaacgc tggcacacga aactgacgtt gaaacgttat
    15481 cgaaagaaga cctggtcgga cgcgtttacg aatacttcct cggtaagttt gccgcaaccg
    15541 aaggcaaagg cggtggtgag ttctacacgc ccaaatgtgt ggttacgctg ttaactgaaa
    15601 tgctcgaacc cttccagggc aaaatttatg atccgtgctg cggctcggcg gggatgtttg
    15661 tgcagtcggt gaagtttgtt gagagccatc agggtaaaag tcgtgatatc gcgctgtatg
    15721 gtcaggaact gacagccacg acgtataaac tggcaaaaat gaacctcgcc attcgcggtc
    15781 tttcagctaa cctcggtgaa cgaccggcag acaccttctt tagcgaccag cacccggacc
    15841 tgaaagccga ctacattctg gcgaatccgc ccttcaacct gaaagactgg cgtaatgaag
    15901 ccgaattaac caaagaccca cgttttgctg ggtatcgtat gccgccaacc ggtaacgcca
    15961 actacggctg gattttgcat atgctctcca agctgtcggc taacggcaca gcaggttttg
    16021 tgctggcaaa cggttcgatg agttctaaca ccagcggtga aggcgagatc cgtgcgcaga
    16081 tgatcgaaaa tgatctgatc gactgcatga tcgccctacc tggacagttg ttctacacca
    16141 cgcagatccc ggtgtgttta tggtttatga ccaaatcgaa ggctgccgac ccagccaaag
    16201 gttatcgtga tcgtcagggc gagacgctgt ttattgatgc gcgtaacctc ggcaccatga
    16261 ttagccgcac aactaaagag ttaacgacgg aagatattgc cacaatcgcc gatacctacc
    16321 atgcctggcg cagcacgccg gaagaactgg ctgcacggat tgcgcgtggt gacagcaagc
    16381 tggaaaaata tgaagaccag gcaggcttct gcaaagttgc gaccctgcaa gatattaaag
    16441 ataacgacta cgtgctgacg ccgggccgct atgtgggtgc agccgagcag gaagaggacg
    16501 gcgtggcatt tgagacaaaa atgcgcgaat tgtcgaagac gttgtttgag cagatgaagc
    16561 aggcggaaga actggatcgt gcgattcgca agaatctgga ggcgctgggt tatgggcttt
    16621 gattttcagt tgttagaaaa tgcagggatc cagctaattg atggtgacag aggcaaaaat
    16681 tatcctaaac ataatgattt tatggagaat ggctattgtc ttttcttatc cgctaaaaat
    16741 gtcactaaat ctggttttca atttcaagaa acattattta tcaatgaaac taaagatcgt
    16801 gaactaagag caggaaagct taagtacggt gacattgttc ttactacccg tggcacagta
    16861 ggtaatgttg cgtactatga caataataat ccatataaac atattagaat caactcagga
    16921 atgattatca ttcgagctga taataaatta tggaacccta aatttctata tttcatatta
    16981 aaatcagaac tattaaaaga acagatcatt aatcttatct ctggctctgc agtgcctcaa
    17041 ctacccgcga gaga
//
LOCUS       DAZFFE010000100        16981 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFFE010000100 DAZFFE010000000
VERSION     DAZFFE010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.100"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(284..454)
                     /locus_tag="ACHJ69_003677"
                     /pseudo
     CDS             complement(284..454)
                     /locus_tag="ACHJ69_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082154.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            810..1688
                     /locus_tag="ACHJ69_003678"
     CDS             810..1688
                     /locus_tag="ACHJ69_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5016573.1"
                     /translation="MKILPVVSTKGGEGKSTQSANLAGFLADAGIRTLLIDGDHAQPT
                     ASNIFPLTYEAPAGLFELLMRTADLTHPDNIISRTAIDGLDLIVSNDPHEQLKTAMLH
                     APDGRLRLRNVLQHPLFLNNYDVIVVDSQGARSVMLEMIVLSATESVVGMVNPVLPDV
                     REFIRGTVNVMENLLPYRELGIPLPKVRTLVNCMDYTALARKTLDELAGIIQTGRYSR
                     QLPEGAVSLLSTQIYDLNIYKQGHAAGQPVHRLEKESTRRSDSALVTMHSLACELFPE
                     WKPAFDGLLENGGIRQ"
     gene            complement(2119..2592)
                     /locus_tag="ACHJ69_003679"
     CDS             complement(2119..2592)
                     /locus_tag="ACHJ69_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016574.1"
                     /translation="MNTEKQLPSGIDNNHDEDGNMAKYYENREAGGMSTDDVERCKTF
                     FDQGTEIFPGADQKLAYRLCWPGHWLINSRRWFFLDDNSISIQQAVKFALIHQHHWLP
                     ATVEQMTPAEMSLALTDYWADYWTQPTEPEKALVHMTDWQYQKLCSNRKKVNGDQ"
     gene            2820..3668
                     /locus_tag="ACHJ69_003680"
     CDS             2820..3668
                     /locus_tag="ACHJ69_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016575.1"
                     /translation="MSSSVKYSPVPAVLTRPELLAELNLLMTRIASGCRVGVPASCRP
                     VIAVHGGYVSYSSPRAGRVFQDGSHPEIVMAVSNRERMCEWFSQVWLPLHLAALEGDE
                     SAFVSAEVNMVRRELQRVPGSSVNCMCWASLAALAFDAEERVELQKGNNENIATYDES
                     IATYAFRAACTVSLCLSRNVSGGIPRSYPEWKAILFPRDGADIFPDGWLENMTRRAER
                     VFTSAKRQCASEQCSGRIQADSHHSGSHPDVSAYEVRCAQEMSLSPVHSTRQCSVQST
                     DSAETE"
     gene            4016..5404
                     /gene="dnaB-PI"
                     /locus_tag="ACHJ69_003681"
     CDS             4016..5404
                     /gene="dnaB-PI"
                     /locus_tag="ACHJ69_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPI-7-type island replicative DNA helicase"
                     /protein_id="HFO5016576.1"
                     /translation="MAERYIPRSVDAEQAVLGGLMLDNDRWDEVVLMLRGEDFSLAVH
                     RVIFRSMSELAVSGQPFDLITLSEHIEKTHNIEALGGFAYLAELSKNTPSAANIMAYV
                     RIVAEKSMLRQLQETGNSLVADVASPDATPRAVLESAERRLFSLSQTGMLHERSEVSL
                     SSAMELALRQLEEGLRAGGMTGTPTGFDELDEMTCGLQPGDLILLAARPSMGKTALGL
                     TLCLNALRERTDETVFLFSIEMSREQLILRTLSMLSRVELTRLRSSMMDDEEWARFTA
                     TMGEFMQEGTFAGEGNRLIIDDTSSQTPSSLRASARRYVRLYGRPSLIMVDYLQLIRS
                     PDQENRTQEIAEISRALKSLGKELGCPVLALSQLNRQVEQRADKRPNNGDLRDSGALE
                     QDADLIMFIYRDEVYNPGTLDKGVAEIIVSKQRQGPTGTIRVSFDGRYTLFSPFERGW
                     NFDRSGGNAYVN"
     gene            5394..7019
                     /locus_tag="ACHJ69_003682"
     CDS             5394..7019
                     /locus_tag="ACHJ69_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO5016577.1"
                     /translation="MSIKRNHSLDLGAAIMQPGRQASKTGNVVALGETPMVLTLDQLR
                     PNPDNPRTTRNPRYDDIKSSIFSRGLDTVPKVTRIPESEPDIYIFSDGGNTRYQILRE
                     LWEETGEQRFYRIHVLFKPWPGRLQCVIGHLAENEVRGELTFIEKAQGIHKARMIYEE
                     QLSKSVSMRELSSLLTEQGLPVDASSISRMENALKYLYPWIPELLESGMGRPQVNSLL
                     SLRQDAERIWGQFAVAAENDVDFDRVFGECCGKFNSPELWSMEMFRDELIGDLLKALP
                     HPVLDYDRWLLELDPRERNRRHHFGEPEPVLMPADSPPEPGAQVASGGATEAAQVVAA
                     TPADSESVMDTAQSGAEKEDRPVADKRERRRKEVQPDMYGGEDSYSGDCGEDATGAST
                     DIPIMPEDNAMQEGGEFRQASAFTGAEQDDIESLQNEAFHLAWELASHTGCVDEIAMD
                     RESELSAGFGAAEANCSPVMAFLVRLTGEEVLRVNPVSLDALLIGGSTEEDLPLLDDE
                     HAGKLLRLLGVLRRLRGLQRMVEDESEEGRDEC"
     gene            7009..7740
                     /locus_tag="ACHJ69_003683"
     CDS             7009..7740
                     /locus_tag="ACHJ69_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFO5016578.1"
                     /translation="MNADAARQDQLIKRIRKLLALSRNNSNPHEAGQALSRAQALMRK
                     HGITELDTDISVIKTSSSKGAPSEAEKVPVWMGRLMHVVAEAFGCHTYLDWRITTGRY
                     WRRVVIFYGFGERPVVAAYAFDVLCRQMQKATKAYLSTQSRTLKLSTRRARAAMFRDG
                     WVYGVRDVVDAFPLTEDETRVMKTWLKRQQFEGMAAFRNVKDCNGGDRARWQGYSEGK
                     KATLHHGVGSRNLRPESLISGGGEV"
     gene            7737..8315
                     /locus_tag="ACHJ69_003684"
     CDS             7737..8315
                     /locus_tag="ACHJ69_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFO5016579.1"
                     /translation="MSNNLSQATNGLLMQLVMDLKSGYLRRCEALGLSREEMQMLQGL
                     SIEEIHYLSNSEVSVLRLDINHNNLVRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FGLSSVDVAARRRISGIDVRPGRGITLSDDENSELWRLWQKAGINDVESVDGLDVMML
                     CAEQMNIPLTAIWHAVRGWHTTGNPDQVRSAS"
     gene            8312..8560
                     /locus_tag="ACHJ69_003685"
     CDS             8312..8560
                     /locus_tag="ACHJ69_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016580.1"
                     /translation="MKMTELVPGVLTSPGVPPASQRKAVELVNRFRRGERNYHELKER
                     GFGYFKIDVGPDWRLLSRNKGLTWALLSHERYNNMRLK"
     gene            8570..8827
                     /locus_tag="ACHJ69_003686"
     CDS             8570..8827
                     /locus_tag="ACHJ69_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016581.1"
                     /translation="MSNAARLQLGFSPFSKTIVLAKMRNFDDGTSLRVGNDPGRDVTN
                     EAAQLVWHLVMAEGGEIMWEMDDGSRMVLKAESQEATSEQD"
     gene            8862..9422
                     /locus_tag="ACHJ69_003687"
     CDS             8862..9422
                     /locus_tag="ACHJ69_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5016582.1"
                     /translation="MHDDWGFDTDLFHELVTPSIVQALLDEREAQSKHVAELEANLSA
                     MTEDHQKATESIKQADSAVKLAHETFSALAAENAKLKKFCKDAAFDADYEAESGMERG
                     GFSDALNEIKITATDAFLAEVRAQGVDAAIDAAKNLVAQEYEYKDFKAAQSDCCMFPG
                     SDLVGKVEMTEWLVDFAAELRKGGNQ"
     gene            9419..10156
                     /locus_tag="ACHJ69_003688"
     CDS             9419..10156
                     /locus_tag="ACHJ69_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5016583.1"
                     /translation="MNKIDYQALREAAEAIKIAATPQKLLAFRMKVTPQVVLALLDEL
                     ETKEEQRANWFQMAQKLGEDLDAEEKRNAEQREYYEGVIADGSKRIAELEKSEEQLIN
                     ERDHAESALADMYFAVTRNRPEWSNCFSFSDAVDAVVDRIADLEAKQPSPVVPEEMYW
                     QDAPVEGSSKATAYATGWNACRAATLQGPEGLIKAVRFYEQVKRENPPVETGAWKDAV
                     DWVLQEACQAVHWCRQISALQEQENHS"
     gene            10153..10368
                     /locus_tag="ACHJ69_003689"
     CDS             10153..10368
                     /locus_tag="ACHJ69_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016584.1"
                     /translation="MRKISGMLNLEKARMQLRVLLSGPVPAITPMAHAVAIKVVLDAL
                     DASERRVAELEQVCTAAGIRIKGEQVK"
     gene            10365..10592
                     /locus_tag="ACHJ69_003690"
     CDS             10365..10592
                     /locus_tag="ACHJ69_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016585.1"
                     /translation="MKLKMQTISGPVIIDSNEVTQFYPDHESGGELTAVEYLTDGGRC
                     TTRVRHSFYQVAEALASAWHVDDASKRNRRG"
     gene            10856..12142
                     /locus_tag="ACHJ69_003691"
     CDS             10856..12142
                     /locus_tag="ACHJ69_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFO5016586.1"
                     /translation="MALPAESLIAHTLRKMKASMAEKASREEGRIRSGLLFTGNVHDA
                     VPRRLLLDTRLSPLDKTAWMMIRLHALSNEGAVFPTYDELQLQLASPGSGKASRETVS
                     RVLTMLRLTGWLSLCKRVRDEKGRIRGNIYALHDEPLGCCDAEKFDPRYLDMVLDSAC
                     KNRNKTIRYTAREVLSDIKNEPGMRHYHSHITLFESRMGAAQTPSQMAARQRVNPAVN
                     GRAAKSGSESAQPPVCASVTELSAQVTELCTAVTEPGEKTEVTQRSTDSEQPGKSVGY
                     HSVRKPNHYVRNITHSMKKNTYVLPDALKSQLSVEDTGMLTKQLQALPETLAEGVLDS
                     LSQMLSKNTLQNPVGWMLAVMKRAREGNFNSPDMSRSGTIQSQPARPSSQSGHGCDER
                     ERRRREVASPEKARNIVAEFRASIQRANAARLAMRG"
     gene            12621..13340
                     /locus_tag="ACHJ69_003692"
     CDS             12621..13340
                     /locus_tag="ACHJ69_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFO5016587.1"
                     /translation="MPEEIVKEPASHRVGAFTSTLNIELHSRYAIMLWEGRRGSKAKN
                     NEGRKVPRIMSMPEAIKMTGGLYRDSLADNPYADETMWKLENAIIDATEKINAEVAQL
                     DDILRNIPSGITIKDVLSRSPVNIGVFSRSPLGYRCVWLLVGYDQLALKTFQAAHLGL
                     ISRNQRDELLSFGAYLIRKIYGILQIYRSVGVTRDDIENKTSKGEKAIARYGMPEPDI
                     LSGKARSSFSPPLNRKSRNLS"
     gene            13363..15375
                     /locus_tag="ACHJ69_003693"
     CDS             13363..15375
                     /locus_tag="ACHJ69_003693"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO5016588.1"
                     /translation="MRLFLCEKPSQASDIARVLGAARRGEGLIEGNDFVVTWAFGHLL
                     EQAPPEAYGEQYGSPWSIEVLPVIPEQWQMVVKDKVARQFNVIRRLLGKADEVVIATD
                     ADREGEIIARELLDYCGYTGVVRRLWLSALDESSIREALGRILPGEETVRLYEAGKGR
                     SRADWLTGINLTRLYTMKARECGVSGVLSIGRVQTPTLAMVVRRDAEIKNFIPVPYWQ
                     LNLWLEKDNVRFRAAWVAAEQYCDVEKRCVNVQAATSVQSQCRQAGVAVVREVSSKRE
                     KTPPPLCFSLGTLQEVCSKKFGLGAQAVLDIAQSLYEKHKATTYPRTDCGYLPVSMHK
                     EVPEVLAAVANTDPDIAPELAKLSPDIVSRVWNDKKITAHHAIIPTRQAFNIGALSDN
                     ELRVYRLIRQHYLAQFLPVQEADVTEATFVVAGQLFGTRGRVEVVKGWRALFIADAPH
                     DEDEGGDTEQTLPLPVLAKGDECRLVEAELKEMKTTPPRHYTEGTLIAAMKNAAGMVS
                     DPALKKVLRENAGLGTEATRAEILETLFTRKYIEKQGKHIRATQLGAELIAGLPEVLT
                     SPGTTALWEQALDDIAQGKLSLASFMEKQKQWLNHLVSGARRQQLQITAPVTPECPLC
                     QGRMRKLKGKNGEFWSCQRYPECKGVISEQKTSHSKRGGRKIKKRG"
     gene            16000..16464
                     /locus_tag="ACHJ69_003694"
     CDS             16000..16464
                     /locus_tag="ACHJ69_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFO5016589.1"
                     /translation="MSATNTSSAKAEYFNLTVKGLGYLSNVCQVNHQNGSFLCCVINA
                     LSGPTDNPSYTRFDVTVVGKDATTLISRCQKAVDEDRKVLLGFALSNLATDIFTLNKG
                     EHAGEQRVSLKARLIKVDWIKIGQEQVYKAEKAESLPPQNGTTTRQYAENSF"
     gene            16558..16764
                     /locus_tag="ACHJ69_003695"
     CDS             16558..16764
                     /locus_tag="ACHJ69_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016590.1"
                     /translation="MNRQNGILAAAAGYTLPLEILKSTRGYYIGTQCSEGPVSRESEE
                     YFKKHDQAEQALKNGTWTQRYDWQ"
     gene            16774..>16981
                     /gene="ssb"
                     /locus_tag="ACHJ69_003696"
     CDS             16774..>16981
                     /gene="ssb"
                     /locus_tag="ACHJ69_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5016591.1"
                     /translation="MASRGVNKVILLGHLGQDPEVRYFPNGGSAVANLSLATSDTWKD
                     KQSGEMKEVTEWHRIVIYGKLAEIA"
BASE COUNT         4209 a         3786 c         4844 g         4142 t
ORIGIN      
        1 ttatttctgg taatgaatgc ggaattaaat ctggccggat tgtatttttg ctatatctgt
       61 ttttcaggag attattatta aatagaaaaa taacagtggt gattatctcc ggttgagctg
      121 gagtcacgta tgccagcgag tgctttcttc cgggccttcc ttcacttcag ataaagagcc
      181 tatggaaaag aggcaatcat caggctcttt ttgagtcata tgcgccgata atcaactgtc
      241 ttgaacgtaa aaattatgga gcaacatgat gctacgattc atttcactct tcgttctttc
      301 tgagcattgc gttcaggatg gtcagcaatt tacgcataca gtcaaccagc gccacttttt
      361 tggtttttcc ggctgctgac agatgcatgt aaaatgcctt tatcaccgga ttaaaccagg
      421 ttaccacaag cgctgccatg tgcagggctg ctcttgcctc cgtacgcgca ctggcgtcgt
      481 tcggccagca gatatgtgtg ggattacagg gatgttatgt gaactgaact gtgttttgtt
      541 aacgggggat gtttattgat gattcgggag tgtttaagac ctgatggcgt tatgctccca
      601 agtttatttt atataaagat ccaaaagcaa aaacccgcct tgtgggcggg ttctttagaa
      661 tagtggtgcc cggactcgga atcgaaccaa ggacacgggg attttcaatc cccttgtcat
      721 aaaattcaga tagttacggg tatgtcaagt tgtcgttgaa gtctttcatg tgcatccgat
      781 cgcaaaaaac agtcgatgag gacgcagata tgaaaatact tcccgttgtt tcaacaaaag
      841 gtggtgaagg taaatcaaca caatccgcaa atcttgctgg ttttctggcc gatgcaggta
      901 tacgtaccct gctgattgac ggagatcatg ctcaacccac agccagtaat atctttccgc
      961 tgacatatga agcgccagcc ggcctttttg aactccttat gcgtacggct gacctcactc
     1021 atcccgataa tatcatttcc cgcacggcta tcgatggcct tgatctgatt gtctccaatg
     1081 atcctcatga acagttaaaa accgcgatgc ttcacgcccc ggacgggcgt cttcgcctcc
     1141 ggaatgttct gcaacatcca cttttcctga ataactacga tgtcattgtc gttgattctc
     1201 agggggcgcg ctccgtcatg ctggagatga tagtgctgtc ggcgactgag tcggttgtcg
     1261 ggatggtcaa tccggttctt cccgatgttc gtgaatttat tcgcggtact gtcaacgtca
     1321 tggagaacct gctgccctac cgtgaacttg ggatacccct gcctaaagta cgcacgctgg
     1381 ttaactgtat ggactatacc gcgctggcca ggaagacgct ggatgagctg gccggaatta
     1441 ttcagaccgg tcgttacagc agacagttac ctgaaggtgc cgtttccctt ctttccacac
     1501 agatttacga cctgaatatt tacaagcagg gacatgcggc tggtcaaccc gttcatcgtc
     1561 tcgaaaaaga atccacacgt cgcagtgatt ctgcgcttgt cacgatgcac agcctggcct
     1621 gtgaactctt tcctgagtgg aaaccggcgt ttgacgggct gctggaaaac ggaggaattc
     1681 gtcagtgatt caggttaagc gagaaccagt cagctctgtt gctgtggctt atggaggtta
     1741 tcaataccgg aagtcgttat ttcatcagtg tcattaattt tatccattta atcaatatga
     1801 tgagaaggat taaccccatg aataacagca gcaataccac aaaccgtaat acgtcttcac
     1861 ccgttgagtc agcctgtaat gcgttactgg attttattgc ccgggaagtc tttgagaata
     1921 aagcatcgga agaggatgtc agggagcgtt gcaaagtatt tacagcgctg cttaacgaag
     1981 gatttaaagc cctgcttgat gagtcagggc tgaatctgtc gaatcttcgt aaacagtgta
     2041 aggaactgga atctcagata accagactgg attctcagtc cgacgcaatt tgtcgcagaa
     2101 gcgctcaaat tgaacgcttc attggtcacc atttaccttt tttcgatttg aacaaagttt
     2161 ctggtactgc cagtcagtca tgtgaaccag cgccttctcc ggctcagtcg gctgtgtcca
     2221 gtagtcagcc cagtaatctg tcagagcaag ggacatctct gcgggtgtca tctgctcaac
     2281 cgttgccggg agccagtgat gctgatgaat aagagcaaac ttaacggctt gttgaatgct
     2341 gatactgtta tcgtccagaa aaaaccagcg acggctgtta atcagccagt gcccgggcca
     2401 gcaaagtcgg taagcgagct tctggtcagc cccgggaaag atttcagttc cctggtcaaa
     2461 gaaggtttta caacgttcca catcgtcggt gctcatgcct ccggcttccc ggttttcata
     2521 atattttgcc atgttgccat cctcatcatg gttgttatca atgccggacg gcagttgttt
     2581 ttctgtattc ataaaaattc tcctttaaaa caatctgtta tgagtatgcg aatgctgtcc
     2641 gtaccttctg accggggtcg ccaaactcac cggtcggaaa atgtacaggc agttatgggc
     2701 attttatgcc ctgcaactgt tcgtccggcg ccctttttcc tttttttaca cagccaggtt
     2761 tccggttatc tgcggggaag tgacgcacct ggcgggcaga gagatatgga ggtgaaagta
     2821 tgtcttcctc agtgaaatat tctccggttc cggcggtgtt aacccgccct gaattgctgg
     2881 ctgaactgaa cctgctgatg acccggattg ccagtgggtg tcgtgtgggg gttccggcgt
     2941 catgtcgtcc ggtcattgcg gtgcacggtg gttatgtttc atacagttct ccacgcgctg
     3001 gacgtgtttt tcaggacggt tcccacccgg agatagtgat ggccgtcagt aaccgggaac
     3061 ggatgtgcga atggtttagt caggtctggc ttccgctgca tctggctgcc cttgaaggcg
     3121 atgaaagtgc ttttgtcagt gcggaggtca acatggtgcg ccgggagctg cagcgcgttc
     3181 cggggagttc agtgaactgt atgtgctggg caagccttgc tgcactggca tttgatgctg
     3241 aagagcgggt ggaactgcag aaaggcaata atgagaacat cgcaacatat gatgagagca
     3301 ttgcaacata tgcattccgt gctgcctgta ctgtgagtct ctgtctgagt cggaatgttt
     3361 ccggaggtat tccgcgctca taccctgaat ggaaagcgat tttgtttccc cgtgatggtg
     3421 ctgacatttt ccccgacggg tggctggaaa atatgacccg tcgggctgaa cgtgtcttta
     3481 cgagtgcgaa acgtcagtgt gccagtgagc aatgctccgg ccgtattcag gccgacagcc
     3541 atcattcagg gagccaccct gacgtttcag cttatgaagt acgctgtgca caagaaatgt
     3601 cgctttcacc ggtccattcg acccgacagt gttcagttca atccactgat tctgctgaaa
     3661 cggagtgaaa ggatgagccg attcaataca gttcgggggg aaggggttac agagcccgtc
     3721 tttttccgtc atgacctttc cgttactcag cagccagaac tgcagggtgt atttcacttc
     3781 agatccaatg ccggacggca gttgtttttc tgtattcata aagtttttcc tttaaatcag
     3841 attgttatga atgaacgaat gctgtccgta ccttctgacc ggggtcggca aacttaccgg
     3901 tctgaaaacg tacaggcagt tatgggcatt ttatgccctg caactgttcg tccggcaccc
     3961 tttttccttt tttcacatag ccgggtttcc ggcagtcaga gagggaggat acaccatggc
     4021 tgaacgctat attccgcgct ccgttgatgc tgaacaggct gtgcttggcg gcctgatgct
     4081 tgataatgat cgctgggatg aagtggtgct gatgctcagg ggggaggatt tttctctggc
     4141 tgtgcaccgg gtgatattcc gctctatgtc agagctggca gtcagcggtc agccgttcga
     4201 cctcatcacg ctgagtgagc atattgagaa aacgcacaat attgaggcac tgggcggctt
     4261 cgcttacctg gctgagctca gtaagaatac accttcagca gccaatatca tggcttatgt
     4321 ccgtattgtg gcggaaaaga gtatgctgcg gcagcttcag gaaaccggta attctctggt
     4381 tgctgatgtt gcatcgccgg atgccactcc gcgcgcagtt cttgaatccg cggaacggcg
     4441 acttttttct ctttcccaga caggcatgct gcatgagcgc agtgaagtga gcctttcctc
     4501 cgcaatggag ctggccctgc gtcagctgga agaaggactg agggcgggcg ggatgacggg
     4561 caccccaacg gggtttgatg aactggatga gatgacctgt ggcctgcagc ccggggatct
     4621 tatccttctg gcagcacgtc cgtcgatggg gaaaacggcc cttggactca ctctgtgtct
     4681 taatgccctg cgtgagcgga ccgatgaaac ggtattcctg ttcagcatag aaatgtccag
     4741 ggagcagctg attctgcgta ccctgtcgat gctgtcccgg gtggagctga ctcgtcttcg
     4801 cagcagcatg atggacgatg aggagtgggc gagatttacg gcaacaatgg gtgagttcat
     4861 gcaggagggg acatttgccg gtgaaggtaa ccgcctgatc attgatgaca cgtcatctca
     4921 gactccgtca tcactgcgcg ccagtgcccg tcgttatgtg cgtctgtatg gcagaccttc
     4981 cctgataatg gtcgattatc tccagttgat tcgttcaccg gaccaggaaa accgcactca
     5041 ggagattgcg gaaatatccc gagccctcaa gtcgctgggg aaagaacttg gatgccctgt
     5101 gctcgcactg tcgcagctta accgacaggt ggaacagcgg gcagataaac gaccaaacaa
     5161 cggtgacctg cgtgattccg gggcgctgga gcaggatgct gacctcatta tgtttattta
     5221 ccgtgacgag gtttacaacc ccggcactct ggataagggc gtggcggaaa tcattgtgag
     5281 caaacagcga cagggaccga ccggtacgat acgggtgagc ttcgatggtc gttacaccct
     5341 gttttcaccg tttgaacgtg gctggaattt tgaccgttcc ggaggcaatg cgtatgtcaa
     5401 ttaagcgaaa tcattccctt gacctggggg ccgccatcat gcaaccgggg cgccaggcca
     5461 gtaagaccgg taacgtggtt gccctggggg aaacgcccat ggttctgacg ctggatcagt
     5521 tgcgccctaa tcctgataac ccgagaacca cgcgcaatcc ccggtacgac gatattaaaa
     5581 gttcaatttt ctcccgcgga ctggatacgg tgccaaaggt cacccggata ccggagagtg
     5641 agccggatat ctacatcttc agcgatggtg gtaacactcg ttatcagatc ctgagagaac
     5701 tgtgggagga gaccggcgag cagcgttttt accgtattca tgtgcttttc aaaccgtggc
     5761 cggggcggct tcagtgtgtc attggtcatc tggctgagaa tgaagtcagg ggcgaactga
     5821 cgtttattga aaaggctcag ggtatccata aagcacggat gatttatgaa gagcaattaa
     5881 gcaaaagtgt gtcgatgcgt gaactgtcat ctcttttgac tgagcaggga ttgcctgtgg
     5941 acgcatccag tatcagccgg atggagaacg cactgaaata tctgtatccg tggatcccag
     6001 aactactgga atcaggaatg gggagaccgc aggtcaattc tttgttatct ctccgtcagg
     6061 atgcggaacg catctggggg cagtttgcag tcgcggcgga aaacgatgtg gattttgatc
     6121 gcgtgtttgg tgaatgttgc ggcaaattca actcgccgga actctggtcc atggaaatgt
     6181 tcagggatga gctgatcggt gacctgctga aggctctgcc acatccggta ctggattacg
     6241 atcgctggtt gctggagctg gatccccggg agcgtaatcg acgtcaccat tttggtgaac
     6301 ccgaaccggt actcatgccg gcggacagcc ccccggaacc gggggcgcag gtggcgtcag
     6361 gcggggcgac ggaagctgct caggtcgttg ccgccactcc cgcagacagt gagagcgtca
     6421 tggatactgc gcaatcaggg gctgagaaag aggaccgccc ggtcgcagat aaacgcgaac
     6481 gacgcagaaa ggaagttcaa ccggatatgt atggtggcga agactcatac agcggtgatt
     6541 gcggagagga tgcgaccggt gcgtcaactg atataccgat aatgccggaa gataacgcca
     6601 tgcaggaggg gggagaattt cgccaggcat ctgcattcac tggtgctgaa caggatgata
     6661 tcgaatctct gcagaatgag gcgtttcacc tcgcatggga gctggcgtca catacaggct
     6721 gtgtcgatga gatagcgatg gaccgggaaa gtgagctgtc tgcaggattt ggtgcggcag
     6781 aggcaaattg ctcaccggtc atggcatttc ttgtcagact gaccggtgag gaggttctgc
     6841 gggtcaatcc ggtttccctt gatgcactgc tgataggcgg cagtactgaa gaagacctgc
     6901 cgttgcttga tgatgaacat gcaggaaagt tgcttcgcct gctgggtgtg ttgcgtcgtc
     6961 tgcgggggct gcagcgtatg gtggaagacg agagtgagga agggcgcgat gaatgctgat
     7021 gccgcacgtc aggatcaact gattaaacgg ataagaaaac tgctggcgct gagccggaat
     7081 aacagtaacc cccatgaggc aggccaggca ctttcccgtg ctcaggcgct gatgcgcaag
     7141 cacggtatca ctgaactgga tactgatatt tcggtgataa agacctcgtc atcaaagggg
     7201 gccccgtcag aagcggaaaa agttccggta tggatggggc gccttatgca tgttgttgca
     7261 gaggcattcg gatgccatac atacctggac tggcgtatca caaccggaag gtactggcgg
     7321 cgggttgtca tattttatgg ctttggggag cgcccggttg tggcggcgta tgcctttgat
     7381 gttctctgcc ggcagatgca aaaggcaaca aaggcgtatc tttcaaccca gagccggaca
     7441 ttaaaattgt caacccgaag ggcaagagca gccatgtttc gtgacggctg ggtttatggg
     7501 gtgcgtgatg tggtggatgc attcccgctc acggaagatg agacgcgtgt aatgaagacg
     7561 tggctgaaga gacagcaatt tgagggaatg gctgccttcc ggaatgttaa ggactgtaat
     7621 ggtggtgatc gtgcacgctg gcagggatac agcgagggta aaaaggcaac actgcatcat
     7681 ggtgttggtt cccggaatct gagacctgaa agcctgatat cgggcggagg tgaggtatga
     7741 gtaacaactt atcacaggcc accaacgggc tgttgatgca actggtgatg gatctcaaga
     7801 gtggctatct gcggcgatgt gaagcgctgg ggctttcccg ggaagaaatg cagatgctgc
     7861 aggggctgag cattgaagaa attcactatc tgtcaaattc agaagtgtcg gtgctgcggc
     7921 tggatattaa ccacaacaat cttgtgcgca tgttgcagca ggcgcggact gaacagaaac
     7981 gtctgcagcg aatcgatcgc gctctggcgc tgggtgggtc tatcgaactc atggcgtttt
     8041 attttggtct ttccagcgtg gatgtggctg cgcggcgtcg tatctccggt attgatgttc
     8101 gtcccggaag ggggatcacc ctgagtgacg atgagaattc tgaactctgg cggttgtggc
     8161 agaaagccgg gatcaatgat gtggagagtg tggatgggct ggatgtcatg atgctttgtg
     8221 ctgaacagat gaacattcca ctgacggcca tctggcatgc cgtcagaggg tggcatacaa
     8281 ccggaaatcc ggatcaggta aggagtgcgt catgaagatg acagaactgg ttcccggagt
     8341 gctgacaagt ccgggcgtcc ctcccgccag tcagcgtaag gcggttgaac tggtcaatcg
     8401 cttccgtcgt ggagagcgga attatcatga actgaaggaa aggggatttg gttatttcaa
     8461 gattgatgtt ggtcctgact ggcggttact gagccggaat aaagggctga cctgggccct
     8521 gttgagtcat gaacgataca acaacatgag actgaagtga ggtggaaaga tgagtaatgc
     8581 agcaagactc cagctgggtt tttctccgtt ttcaaaaact atcgtgctgg caaaaatgcg
     8641 taattttgac gacggaacca gtcttcgtgt cggtaatgat cccggtcgcg atgtaaccaa
     8701 tgaggcggct cagcttgtct ggcatctggt catggcagaa ggtggtgaaa ttatgtggga
     8761 aatggacgat ggctcccgca tggtgctgaa ggctgaaagt caggaggcaa ccagtgagca
     8821 agattaacta ccaggcactg cgtgaggcgg cagagcaggc aatgcatgac gactggggat
     8881 ttgacacgga tcttttccat gagctggtaa caccatcgat tgtgcaggcg ctgctggatg
     8941 aacgggaagc ccaaagcaaa cacgtagctg aactggaggc taatttatcg gcgatgaccg
     9001 aagaccacca gaaagcgact gagtcaatta agcaggctga ttcggctgtt aagttggcac
     9061 acgagacgtt ttcggcgctg gcggcggaga atgcaaagct gaagaagttc tgcaaagacg
     9121 ctgcattcga tgccgattac gaagcagagt caggtatgga gcgtggtgga ttcagtgatg
     9181 cgcttaacga aatcaaaatc acagccaccg acgctttcct ggctgaagta cgggcgcagg
     9241 gggtggatgc tgctatagac gctgcaaaaa atctggtggc tcaggaatat gagtataagg
     9301 atttcaaagc ggcgcagagt gattgctgta tgtttcctgg ttcagacctg gtagggaagg
     9361 ttgaaatgac cgagtggtta gttgactttg ccgcagagct tcgcaaagga ggcaaccagt
     9421 gaacaagatt gactatcagg cactgcgtga ggcggcagaa gcaataaaaa tagcagccac
     9481 accacaaaaa ttgctggcat ttcgtatgaa agtcacacct caggttgtgc tggcgctgct
     9541 ggatgagctg gaaaccaaag aagaacagcg agctaactgg ttccagatgg cgcagaagtt
     9601 aggcgaggat ttggatgcag aagaaaagcg gaacgccgaa cagcgtgagt attacgaggg
     9661 agtaatcgcg gatggaagta agcgcattgc agaactggag aaaagtgaag agcaactcat
     9721 taatgagcgt gaccatgctg agtctgcttt agctgatatg tactttgcag taaccaggaa
     9781 caggcctgag tggagtaact gtttcagttt ttcagatgcc gtcgatgccg tggttgacag
     9841 aattgctgat ttagaagcta aacagccatc gccagtggtg ccggaagaaa tgtattggca
     9901 ggatgcacca gtggaaggca gcagtaaagc gactgcatac gcgacaggct ggaatgcttg
     9961 tcgtgccgcc acgcttcagg gaccagaagg attgattaaa gcagtgcgct tctatgaaca
    10021 ggttaagcgt gaaaatccgc cagtcgaaac cggagcatgg aaagacgctg ttgactgggt
    10081 attacaggaa gcatgtcagg ctgttcattg gtgccgacag atatcagccc ttcaggaaca
    10141 ggagaatcat tcatgagaaa aatcagtgga atgttgaatc tggagaaggc caggatgcag
    10201 ttgcgtgttt tgttatctgg acctgttcct gcaatcactc cgatggcgca tgccgtggca
    10261 ataaaagttg tgcttgatgc tctggatgcg agtgagcgga gggtggctga actggaacag
    10321 gtctgtactg ccgcgggtat tcgtatcaaa ggggaacaag tgaaatgaaa ctgaaaatgc
    10381 agacaatttc gggtcctgtg attatcgaca gtaatgaggt gacacagttt tacccggacc
    10441 atgaaagtgg aggggaactc accgctgttg aataccttac tgacggggga agatgcacca
    10501 cccgggtacg ccattccttt tatcaggttg ctgaggcact tgccagtgca tggcatgtgg
    10561 atgatgcatc aaaaaggaat cgccgtggat aacctgaggt gccgggggta tgtcagttct
    10621 gaagctgctg aaccgtgaca taaatttttt atgttgcggt gaagcagcca acaaacgaag
    10681 tgcgtcagcg tgctggttat taaggcaata ttttttcaga ggaaccgcaa taccgtcggt
    10741 attaacggtt tacgggtgat gaagtgttta gcctgcatct ttgccgggtg cagttataag
    10801 catttcagta acagaacccc ggtgttgttt tccgggattg aaggagaatt gaatcatggc
    10861 gttaccagca gaaagcctta ttgcgcatac gctcagaaaa atgaaggcca gcatggcaga
    10921 gaaagccagt cgtgaggagg gaaggatacg cagtgggctg ctgtttaccg gtaatgttca
    10981 tgatgcggtg ccccgtaggc tgctgctgga cacgcgcctt tctcctcttg ataaaacggc
    11041 atggatgatg attcgtctgc atgcgctgag taatgagggc gcagtattcc ccacctacga
    11101 tgaacttcag ttgcaactgg cctcacccgg tagcgggaag gcatcccgtg aaaccgtaag
    11161 ccgggtactg accatgcttc ggctgaccgg ctggctgagt ctgtgtaaac gggttcgtga
    11221 tgaaaaaggc cgtatccgtg gaaatatcta cgccctgcat gatgaacctc ttggctgttg
    11281 tgatgcagaa aagtttgacc ctcgctactt ggatatggtg ctggattctg catgcaagaa
    11341 caggaacaag accatccggt atacagccag agaggttctc agtgatatca aaaatgaacc
    11401 tggtatgcgg cattaccaca gccatatcac gctgtttgaa tcacggatgg gcgcagcgca
    11461 gacaccgtct cagatggcag cgcgtcagcg ggtaaatccg gcggtgaatg gtcgtgcagc
    11521 gaagagcggt tcggaatccg cacagccccc tgtatgtgcg tcggtaaccg aactcagtgc
    11581 tcaggtaacc gaactctgta ctgcggtaac cgaacccgga gaaaaaacag aggtaacgca
    11641 acgcagtacg gattccgaac aaccaggaaa atcagtgggt tatcactcag tacggaaacc
    11701 gaaccactac gtacgtaata ttacacacag tatgaaaaaa aatacgtacg tattgccgga
    11761 tgcactgaaa tcacaactca gcgtggaaga caccggcatg ctgacgaagc aactgcaggc
    11821 gttgccggaa acgctggcgg aaggtgtgct ggacagtctg tcacagatgc tcagcaaaaa
    11881 tacgcttcag aatcccgttg gctggatgct ggctgtgatg aaacgtgcgc gtgaagggaa
    11941 ctttaactcg ccggatatga gcagaagcgg aacgatccag tctcagcctg cccgtccatc
    12001 atctcagtcc ggacatggat gtgatgaacg tgaacgtcgt cgcagggaag tggcttcgcc
    12061 agaaaaagcg agaaatattg ttgcagaatt ccgtgccagc atacagcgtg ctaatgctgc
    12121 ccgtctggct atgcgtggtt aagtgctggc cgggatgttt cctgttgtgg cgaacactgt
    12181 tggtagtgcc atcagtggct aaaaagtgaa cggtgttgcc agtggtaacg ctgattaaaa
    12241 aatgagcgat actgccagtg gcaatagtga ttaaaaagtg aacggcgttg ccagtggcaa
    12301 cagtggttaa aaaacgagca gtgttggcag tgccaacggt gactaaaaag tgaacggtgt
    12361 tgccagtggc aacagtgatt aaaaagtgaa cggtgttgcc agtggcaacg ctgattaaaa
    12421 aatgagcgat gttgccagtg acaacagtga ttaaaaagcg agcggtgttg gcagtaccaa
    12481 cagtgactaa gaaatgagcg cttttgccaa tgctactgtg cgtaatttca gtcatctctt
    12541 tttttatttt cgattgtcag atctttgtcg gtgagaggac tatttctgtc tgatgtcatt
    12601 ttcaatagtg gagatacaac atgccagaag aaatagtgaa ggagccagcc tctcatcgtg
    12661 tgggagcttt tacatcaact ctgaatatag agctacattc ccgctatgcc attatgttat
    12721 gggaaggtcg acgtggctca aaagctaaaa ataatgaggg gcgtaaagtc ccacgaatta
    12781 tgagtatgcc agaagccatt aaaatgactg gtggtcttta ccgggattct cttgctgata
    12841 atccttatgc tgatgagacg atgtggaaac tggagaatgc gattattgat gcgactgaaa
    12901 aaattaatgc cgaagtagcc cagcttgacg atattctcag aaacatacct tccgggatta
    12961 ctatcaagga tgtgctttcg cgttcaccag tcaatatagg ggtattcagt cgttcgccgc
    13021 ttggttaccg ttgtgtctgg cttctcgtcg gatatgatca gcttgcatta aaaacgtttc
    13081 aggcggctca tctggggtta atttcccgaa accagcgtga tgaactgttg tcgtttggtg
    13141 cgtaccttat ccgcaagatt tacggtattc tacaaatcta tcgttctgta ggtgtcacgc
    13201 gggatgatat tgaaaataaa accagtaaag gtgagaaggc tattgctcgt tacggtatgc
    13261 ctgagccaga tattctttct ggtaaagcta gatcttcatt ctctccccct ctgaaccgta
    13321 agtcacgtaa cttatcatag cagtgagttg ccagggggaa ttatgcgtct ttttttgtgt
    13381 gagaaacctt cacaggccag tgacattgcc agagtgcttg gggctgccag acgcggtgaa
    13441 ggcctgattg aaggtaacga ctttgttgtg acatgggctt ttggtcattt gctggagcag
    13501 gctccaccgg aggcgtacgg tgagcagtat ggcagtccct ggagcattga ggttctgccg
    13561 gttattcctg aacagtggca gatggtggtg aaggataagg tcgccaggca gtttaatgtt
    13621 atcaggcgtc ttctcggtaa ggcagatgaa gtagtgattg ccaccgatgc tgatcgggaa
    13681 ggcgaaatta ttgccagaga actgcttgat tattgcggtt atacaggagt agttcgacgc
    13741 ctctggttat ccgcactgga tgagagcagt atcagggaag cgctgggcag aatacttcca
    13801 ggagaggaaa ctgtgcgttt atatgaggcc ggtaaggggc gcagccgggc agactggctg
    13861 acggggatca acctgacgcg tttgtacacg atgaaagcca gggagtgcgg cgtgtctggt
    13921 gttctctcga ttggcagggt gcagactccc acgcttgcga tggtggtcag acgggatgcg
    13981 gaaattaaaa attttatccc ggtaccttac tggcaactga acctctggct tgagaaagac
    14041 aacgtgcgtt ttcgcgcagc ctgggttgcg gcagagcagt actgtgatgt ggaaaagcgg
    14101 tgcgtgaatg tgcaggctgc cacaagcgtc cagtcgcagt gtcgtcaggc cggtgttgct
    14161 gtagtcagag aggtcagcag caaaagagag aagacgccgc ctccgctttg tttttcactc
    14221 ggtacactgc aggaggtctg ctcgaaaaaa tttggtttag gtgcgcaggc cgttcttgat
    14281 atagcccagt cgttgtacga aaaacacaag gcaacgacgt atcctcgtac tgactgtggt
    14341 tatctgccgg tatcaatgca taaagaagtg ccggaggttc tggcggctgt ggcaaacacc
    14401 gatccggaca ttgcccctga actggcaaaa ctcagtcctg atattgtttc cagagtctgg
    14461 aatgataaga agatcactgc gcatcatgcg attatcccca cccgtcaggc gtttaatatt
    14521 ggtgccctga gtgataacga actcagggtt taccggttaa tccgtcagca ttatctggct
    14581 cagtttttac ccgtgcagga agcggacgtc actgaagcca cttttgttgt tgccgggcag
    14641 ttgtttggca cgcgaggacg ggtggaagtg gtgaaaggat ggcgtgcgct ttttattgca
    14701 gatgcgccgc atgatgaaga cgaggggggc gatacagagc agacactgcc tctgcctgtt
    14761 ctggctaagg gggatgaatg tcgtcttgtt gaagcagaac tgaaagagat gaaaaccacg
    14821 cctccgcgtc actatacgga gggcacgctc attgctgcga tgaaaaatgc ggcgggaatg
    14881 gtgagcgatc cggctctgaa aaaagtgtta cgcgagaatg ccggactggg cacggaagca
    14941 acccgtgctg aaatactgga gacactgttc acccgcaaat atattgagaa acagggtaag
    15001 catatccggg caacacaact gggggcagag ttgatagcag gattacctga ggtgctgacc
    15061 agtccgggaa ccacggcact ttgggagcag gccctggatg atattgcaca gggtaaactg
    15121 tcgctggcgt catttatgga gaagcagaaa cagtggctga atcacctcgt ctcgggagcc
    15181 cgacggcagc aattgcagat tacggcacca gtgacgccgg aatgcccact ttgccagggc
    15241 agaatgcgga agctgaaagg taagaacgga gagttctgga gttgccagcg ttacccggag
    15301 tgtaaaggcg ttatttcaga gcagaaaacg tcacacagta aacgcggcgg cagaaaaatt
    15361 aaaaagcgtg gatgatgatt acttcctttt ttttatttgt taaagtgcat acgctttgat
    15421 gtataccccc atgatcagtg ggaaacagag cagcgattaa acaacggcgt tgttttgttt
    15481 ctgatccgca acggccagcg tccggttgag ttgcaaggcg tatgaactcc gtgaaaaccg
    15541 cgccgggagt gatccccgga gacaagtgag atgaccgaag ggctggtaac cccatgacgg
    15601 cgctgaagat tcagcgcctt ttttatgctc atttcaaaga gtgaacatct cggtgagata
    15661 attatttaat gacgttttgt tcttgacttt gtgttcaggt gttttaaaaa tgagcatgct
    15721 aaatcgctgc cttgcagcca accaatgtcc tggtgatctg agaaggacgc cttcgggtga
    15781 ttgcaatatc ctcgtagcct gaaagaggat gcctttatgg cggtacatcc gtttaacctc
    15841 tgggaacgga tatcaccaca gttatggtga tcgaggtatg tttaactgca cagacagccg
    15901 gaaatacagc tgattccggc cccccgacgg gaactatcac acccgtcagg ggcggtggtg
    15961 ttctccgtca tatctttttt tatccatgga gaacacatca tgtctgcaac aaatacttct
    16021 tctgcaaaag ctgaatactt caatctgacc gtaaaaggtc tggggtacct cagtaatgtg
    16081 tgtcaggtta accatcagaa tggctcgttc ctttgctgcg tgattaacgc actgagtgga
    16141 ccaacggaca atccgtccta tacgcgtttt gatgttacgg ttgttggtaa agatgcaacc
    16201 accctgatct ctcgctgcca gaaagcagtg gatgaggacc gtaaggtttt gcttggcttt
    16261 gcgttaagca acctggccac cgatatcttc accctgaaca agggagagca tgccggtgag
    16321 cagcgcgtca gtttgaaagc ccgcctgatc aaggttgact ggattaaaat tggccaggag
    16381 caggtctata aggctgaaaa agcggagtcc ctgccaccgc aaaatggcac aactacacgt
    16441 caatacgcag aaaactcttt ctgatcgtat ttccctcagc ctccctgatg atgaaaacag
    16501 accaccatca gggaggtctg ttttcttatc gtttttgcaa actttaagga aacacatatg
    16561 aaccgtcaaa acggcattct tgcagctgcg gctggctata cattacccct ggaaatcctc
    16621 aaaagtacgc gtggatatta tattggcact cagtgttctg aggggccggt gtcccgtgaa
    16681 tctgaagagt atttcaaaaa acatgatcag gctgagcagg cgctgaaaaa tggtacctgg
    16741 acacagcgtt atgactggca atgagggggg agtatggctt cacgtggcgt aaacaaagtc
    16801 atcctgcttg ggcatctggg acaggatccg gaggtcaggt actttcctaa cggtggtagt
    16861 gcagtggcga atttgtcact ggcgaccagt gatacctgga aggataaaca gagcggtgaa
    16921 atgaaggaag tgaccgaatg gcaccgcatt gttatttatg gaaaactggc agaaatcgcc
    16981 g
//
LOCUS       DAZFFE010000101        16883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZFFE010000101 DAZFFE010000000
VERSION     DAZFFE010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.101"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(19..966)
                     /gene="dsdC"
                     /locus_tag="ACHJ69_003697"
     CDS             complement(19..966)
                     /gene="dsdC"
                     /locus_tag="ACHJ69_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DsdC"
                     /protein_id="HFO5016592.1"
                     /translation="MEPLREIRNRLLNGWQLSKLHTFEVAARHQSFALAAEELSLSPS
                     AVSHRINQLEEELGIQLFVRSHRKVELTHEGKRVYWALKSSLDTLNQEILDIKNQELS
                     GTLTLYSRPSIAQCWLVPALGDFTRRYPSISLTVLTGNDNVNLQRAGIDLAIYFDDAP
                     SAQLAHHFLMDEEILPVCSPEYAQRHDLTNTVINLRHCTLLHDRQAWSNDSGTDEWHS
                     WAQHYAVNLPTSSGIGFDRSDLAVIAAMNHIGVAMGRKRLVQKRLASGELVAPFGDMT
                     VKCHQHYYITTLPGRQWPKIEAFITWLREQVSQYECYTL"
     gene            1184..2521
                     /gene="dsdX"
                     /locus_tag="ACHJ69_003698"
     CDS             1184..2521
                     /gene="dsdX"
                     /locus_tag="ACHJ69_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
                     /protein_id="HFO5016593.1"
                     /translation="MHSQIWVVSTLLISIVLIVLTIVKFKFHPFLALLLASFFVGTMM
                     GMGPLDMVNAIESGIGGTLGFLAAVIGLGTILGKMMEVSGAAERIGLTLQRCRWLSAD
                     VIMVLVGLICGITLFVEVGVVLLIPLAFSIAKKTNTSLLKLAIPLCTALMAVHCVVPP
                     HPAALYVANKLGADIGSVIVYGLLVGLMASLIGGPLFLKFLGQRLPFKPVPTEFADLK
                     VRDEKTLPSLGATLFTILLPIALMLVKTIAELNMARESGLYTLLEFIGNPITAMFIAV
                     FVAYYVLGIRQHMSMGTMLTHTENGFGSIANILLIIGAGGAFNAILKSSSLADTLAVI
                     LSNMHMHPILLAWLVALILHAAVGSATVAMMGATAIVAPMLPLYPDISPEIIAIAIGS
                     GAIGCTIVTDSLFWLVKQYCGATLNETFKYYTTATFIASVVALAGTFLLSFII"
     gene            2539..3867
                     /gene="dsdA"
                     /locus_tag="ACHJ69_003699"
     CDS             2539..3867
                     /gene="dsdA"
                     /locus_tag="ACHJ69_003699"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO5016594.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAYAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(3975..5513)
                     /gene="emrY"
                     /locus_tag="ACHJ69_003700"
     CDS             complement(3975..5513)
                     /gene="emrY"
                     /locus_tag="ACHJ69_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO5016595.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(5513..6676)
                     /gene="emrK"
                     /locus_tag="ACHJ69_003701"
     CDS             complement(5513..6676)
                     /gene="emrK"
                     /locus_tag="ACHJ69_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO5016596.1"
                     /translation="MEQVNSNKKHSDRRKYFALLAVVLFIAFSGAYAYWSMELKDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKEALEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVIEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7092..7706
                     /gene="evgA"
                     /locus_tag="ACHJ69_003702"
     CDS             7092..7706
                     /gene="evgA"
                     /locus_tag="ACHJ69_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO5016597.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            7711..11304
                     /gene="evgS"
                     /locus_tag="ACHJ69_003703"
     CDS             7711..11304
                     /gene="evgS"
                     /locus_tag="ACHJ69_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO5016598.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADGDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRIRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEDGEVDIVLSHLVASPPLNDDIAATNPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFLLTRKDSIVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDETGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPAAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVQNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTLPERYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTIFTITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPTAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQQND"
     gene            complement(11360..12505)
                     /gene="yfdE"
                     /locus_tag="ACHJ69_003704"
     CDS             complement(11360..12505)
                     /gene="yfdE"
                     /locus_tag="ACHJ69_003704"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO5016599.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINSRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLVKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(12579..13523)
                     /gene="yfdV"
                     /locus_tag="ACHJ69_003705"
     CDS             complement(12579..13523)
                     /gene="yfdV"
                     /locus_tag="ACHJ69_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO5016600.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(13593..15287)
                     /gene="oxc"
                     /locus_tag="ACHJ69_003706"
     CDS             complement(13593..15287)
                     /gene="oxc"
                     /locus_tag="ACHJ69_003706"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO5016601.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQSDEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAAETQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15341..16591)
                     /gene="frc"
                     /locus_tag="ACHJ69_003707"
     CDS             complement(15341..16591)
                     /gene="frc"
                     /locus_tag="ACHJ69_003707"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO5016602.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         4816 a         3831 c         3597 g         4639 t
ORIGIN      
        1 accagatgat ttaatgtatt ataaggtata acattcatat tgacttacct gttctcttaa
       61 ccaagtaata aatgcctcaa tttttggcca ctgcctgccc ggtaatgtgg tgatgtaata
      121 atgctgatgg catttcaccg tcatatcgcc aaacggcgcg acgagctcac cactggcaag
      181 ccttttttgt accagacgtt tccttcccat cgccacccca atatgattca tcgcggcgat
      241 aacggctaaa tcagagcgat caaagccaat tccagaagat gttggcaaat taaccgcata
      301 atgttgcgcc caactatgcc attcatccgt accggagtcg ttgctccatg cctgtctgtc
      361 atggagcaac gtacagtgac gcaggttaat taccgtgttg gttaaatcat gtctttgagc
      421 gtactccggg ctgcaaactg gcaagatttc ttcatccatc agaaagtgat gagccaattg
      481 cgctgacggc gcatcatcaa agtagatcgc caaatcgatt ccggcacgtt gcaaattgac
      541 attgtcatta ccagtgagca cggtgagcga aatagacgga tatcggcgtg taaagtcacc
      601 taatgcgggc accaaccagc attgggcgat agagggccgg gaatacagcg ttaacgttcc
      661 cgataactcc tgatttttga tatccagaat ttcctgattc agggtatcca gcgacgattt
      721 tagcgcccaa taaacacgtt tcccctcgtg cgttaattcc actttgcgat gggaacgaac
      781 aaacaactga atacctaatt cttcttccag ctgattgata cggtgactta ccgcactggg
      841 gctcagcgac aattcctctg ccgcgagagc gaaggactga tgcctggcag ccacttcaaa
      901 agtatgcagt tttgatagtt gccagccgtt aagcagtcga tttcttattt cacgaagggg
      961 ttccataatc acctcatttt tctcttaagt gtaaaaaaat agcggcaaaa tttcagctgt
     1021 aaggtgagct aaagtgaacc atatctcaat tcaccttcat ttttagatgt aaatcactct
     1081 cttgatgcaa tttagctcat gtgaaaggca aattttatcg tttgtcagcc tgcgttgttt
     1141 ttttgtccaa tatcatcagg ttaatcacag gggaaggtga gatatgcact ctcaaatctg
     1201 ggttgtgagc acgctgctta tcagcatcgt gttaattgta ctgaccatcg tgaagttcaa
     1261 attccacccg tttctggcgc tgttgctggc cagcttcttc gtgggaacga tgatgggcat
     1321 ggggccactg gatatggtaa atgctattga aagtggaatt ggcggaacgc tggggttcct
     1381 cgctgcggtt atcggccttg gcacgatact gggaaaaatg atggaagtat ccggggccgc
     1441 agaaagaatt ggtctgacac ttcaacgctg ccggtggctt tcagctgatg tcattatggt
     1501 gctggttggc ctgatttgcg gcatcacgct gtttgttgaa gtgggcgtcg tgctattgat
     1561 tcctctggct ttttcaattg ccaaaaaaac caatacctca ttgttaaagc tggccattcc
     1621 gctgtgtacc gcattgatgg cagtgcactg cgtggttccc ccacatccgg ctgctttata
     1681 tgttgccaat aagctgggcg cagatatcgg ttcggtgatc gtctacggtt tgctggttgg
     1741 gctgatggca tcactgatcg gtggcccact tttccttaaa tttctgggtc aacgactgcc
     1801 ctttaaacct gtacccacag agtttgccga tctcaaagtt cgcgatgaaa aaacactacc
     1861 gtcattaggc gcaacgttat tcaccatact gctacccatt gcgctgatgt tggttaaaac
     1921 gattgccgaa ttgaatatgg cacgtgagag tggtttgtat accttgcttg agtttattgg
     1981 caaccctatc actgccatgt ttatcgccgt gtttgtcgcc tattatgtgt tgggtatacg
     2041 tcagcatatg agcatgggga cgatgctcac acatacggaa aatggtttcg gttctattgc
     2101 taatattttg ctgattatcg gggccggagg cgcattcaac gccattttaa aaagcagcag
     2161 tctcgctgat acgctggcag ttattctctc caatatgcat atgcacccga ttcttctggc
     2221 ctggttggtg gcacttattc tgcatgctgc agtgggctcc gctacggtgg caatgatggg
     2281 ggcaacggca attgttgcac ccatgctgcc gctgtatccc gacatcagtc cggaaattat
     2341 tgcgattgct atcggttcag gtgcgattgg ctgcacgatc gttacggact cgcttttctg
     2401 gctggtgaag caatattgcg gcgctacgct caatgaaaca tttaaatact atacgacagc
     2461 gacatttatc gcttcagtcg tcgctctggc gggcacattc ctgctgtcat ttatcatcta
     2521 agcgcaaaga gacgtactat ggaaaacgct aaaatgaact cgctcatcgc ccagtatccg
     2581 ttggtaaagg atctggttgc tcttaaagaa accacctggt ttaatcctgg cacgacctca
     2641 ttggctgaag gtttacctta tgttggcctg accgaacagg atgttcagga cgcccatgcg
     2701 cgcttatccc gttttgcacc ctatctggca aaagcatttc ctgaaactgc tgctactggg
     2761 gggattattg aatcagaact ggttgccatt ccggctatgc aaaaacggct ggaaaaggaa
     2821 tatcaacaac cgatcagcgg gcaactgcta ctgaaaaaag atagccattt gcccatttcc
     2881 ggctccataa aagcacgcgg cgggatttat gaagtcctgg catatgcaga aaaactggct
     2941 ctggaagcgg ggttgctgac gcttgaagat gactacagca aactgctttc tccggagttt
     3001 aaacagttct ttagccaata cagcattgct gtgggctcaa ccggaaatct ggggttatca
     3061 atcggcatta tgagcgcccg cattggcttt aaggtgacag tacatatgtc tgctgatgcc
     3121 cgggcatgga aaaaagcgaa actgcgcagc catggcgtta cggtcgtgga atatgagcaa
     3181 gattatggtg ttgccgtcga ggaaggacgt aaagcagcgc agtctgaccc gaactgtttc
     3241 tttattgatg acgagaattc ccgcacgttg ttccttgggt attccgtcgc tggccagcgt
     3301 cttaaagcgc aatttgccga gcaaggtcgt atcgtcgatg ctgataaccc tctgtttgtc
     3361 tatctgccgt gtggtgttgg cggtggtcct ggtggcgtcg cattcggact taaactggcg
     3421 tttggcgatc atgttcactg tttttttgcc gaaccaacgc actccccttg tatgctgtta
     3481 ggcgtccata ctggattaca cgatcagatc tctgttcagg atattggtat cgacaacctt
     3541 accgcagcgg atggccttgc tgttggtcgc gcatcaggct ttgtcgggcg ggcaatggag
     3601 cgtctactgg atggcttcta tacccttagc gatcaaacca tgtatgacat gcttggctgg
     3661 ctggcgcagg aagaaggtat tcgtcttgaa ccttcggcac tggcgggtat ggccgggcct
     3721 cagcgcgtgt gcgcatcagt aagttaccaa cagatgcacg gtttcagcgc agaacaactg
     3781 cgtaatgcca ctcatctggt gtgggcgacg ggaggtggaa tggtgccgga agaagagatg
     3841 aatcaatatc tggcaaaagg ccgctaatta attttcaatg cagcatcgcc atcctttccc
     3901 tgggtgagcg atgctgccga tggcgcagac ttaagatccc cggtcttacc tgctataatt
     3961 ctctctttat accatcaccc aacgcctttc gctgtaaacg gcggtttcgc aaaccaaacc
     4021 aataccgtaa gtaagataaa agcaatagcc gccatacgga aaatctcatt tgcagaaatt
     4081 gaaagtgact gctgagttat ttcattatta atttcattaa gaactcctga aagcgaacca
     4141 tagtatttat ccataatttg tgacgatgaa ttaaacacgg ggttaaattg atcgatggtt
     4201 gctgtcaact gactatggtg taacgattcg cggcgtcccc acagtgtcat tgtcaacgac
     4261 gtaccaactg atcctgacaa ggtgcgaaaa aaattactca tactcgaggc attggcaaat
     4321 ttattatctg gcaagcctga aaacgaaatc gttgttaagg gtaaaaagaa acaggcaacg
     4381 gcgaatccct gaaaaaactg tggcaaaatg atgcctgtaa aatcaatcgt tggcataaat
     4441 gtcacagaac gccagtaata gcaaaccgca tacatcaaaa aactaaatgt caccaacaac
     4501 cgcatatcaa ttttgttgcc ataacgtcct atcaaaggtg atattaatag cggcatgata
     4561 ccgatgggcg cataggcaag tccggcccat atcgcattat accccatcgt ttcctggagt
     4621 aactgcggca taagaactat cgctccagag taaaataaat acgcgcatgt gatactcaca
     4681 ataccaatgg tgaaattacg ggacttaaac aaactgaggt caagaatcgg attctctgag
     4741 gtcgactccc aaatgactaa agaaatcaga gaaataactg atactactgt tagtattatt
     4801 attgtactcg agttgaacca atccagatcg cgccctttat caagcataat ttgcaagcca
     4861 ccaacaccga gcactaacag ggtcagtcct ggtagattca ttttgaccgg tgaagtctca
     4921 gtttctcgtc ctttaagtaa ggttaagcat aatgtcagga cgataatccc catagggaca
     4981 ttgattaaaa atatccaacc ccagctaaag ttatcgcaaa tatagccgcc caatatcggc
     5041 ccacatatcg gagcgataat cacggtcatt gaccataatg ccagagcaaa tgttcttttt
     5101 tctggtggat aattccttaa taataaactc tgtgacagtg gaattaacgg ccccgccatt
     5161 aacccctgaa cgactctaaa aaatatcagc acatcaagat tggtcgataa actacacatt
     5221 aatgaagaca gcgaaaaaaa ggttactgaa agtaaaaata accttaattc gcctattctt
     5281 tgtgccagcc tgccggtaac agggatcgca atggcatttg ctacgccaaa cgaggtgata
     5341 acccaggtgc cttcgtctgt tgatgctccc agaaagccag atattgtcgg tattgcgacg
     5401 ttagaaatag tggaatccaa catttgcata aacgtcgcta atgacaatgc aatagtgacg
     5461 caccataacg tcccaccggt taatggtgca ggagttgatt tagtgattgc cattaaagtt
     5521 gtccattatg cgaaataatg ttgctaattt ctttttctat cggactggta tcgataacta
     5581 aagccttact ggtataagca ggcatggacg tcacggttga agccagatcg ggcatctcgg
     5641 caatgtcttc attcttcgta tcaatagtag cagtcatcga taaaccaatg cgcaaagggt
     5701 gttccatgag ttcttttgga tccagagaaa cttcaaccgg tacacgctga acgattttga
     5761 tccagttccc tgtcgcattt tgtgcaggta ataaggagaa cgcattgccg gttcccatat
     5821 tgatccctgt cacccgacca tgaaacacaa cattttcacc ataaagatcg ctgataatat
     5881 tgaccgattg accaatccgt acatccgtga gttgtgtttc tttaaagttg gcattaaccc
     5941 acatttgacg cgccggtacg acagccatta gtgattgccc gggactcact gtttcgccga
     6001 cctgaacact tctctgggca atataaccgg taaccggact cttaatatcc gtacgtttaa
     6061 gcgccaacca ggcttctttg gttgcatctg cagcttcaat gacttgtggc tgacggttta
     6121 atggtgtgtt cattactaaa gctttattcg ctttataagc ctggatagca gcattcaatg
     6181 ccgctttgct acttattaac gtatctttgg tatgctccag cgcttctttt gaaataaccc
     6241 cttgcttcgc taaaggcaca cgacggttat aatcttctaa agattgttga tactgaatac
     6301 gtgctgaagc gacttcggca ctgtattgtt tatcctgtaa gtatagttta ttcgtttgcc
     6361 gaacaatatt tgccagatta tttttagctt tattgagtgc gatagtggca tcagttttat
     6421 ccagtgaaac taaaatgtca ccttgtcgaa cgtagttcgt atctttatga ttaacgacag
     6481 tgacactgcc tgagacttgt gcagaaattg gatctgcatt tcccgtgaca taggcgtcat
     6541 ctgtactaat catgtctttt aattccattg accaataggc ataggcacct gaaaacgcaa
     6601 taaataaaac taccgctaac aaagcaaaat actttcttct gtcagaatgt tttttatttg
     6661 aattaacctg ttccactatt atctctcatt tcgcatagat gaaatttatg aattgaatga
     6721 aaagagtatt cattcctcat atcaaacaca gtgcatgttt tgcacattat cccaggaacg
     6781 ttggagggct aatttaaatg aggtgcaaaa aaaacaacag aggactaaac cgcggctttt
     6841 gcaatacaat tcttacgcct gtaggattag tgagaaaact tatagtgctc atttgaaact
     6901 ataaatcatc ggtatcatcc ctgattttat tgttgacatt ttatttatgc cgactattta
     6961 tatggtatac ttgtcgaatt atcttaaagg aagctcaaat cttcttattt ttattgagaa
     7021 aatgagatga taccttatgt ctgtattact acagggagaa gggaaatgct tcattgcaaa
     7081 gggaataatc tatgaacgca ataattattg atgaccatcc tcttgctatc gcagcaattc
     7141 gtaatttatt gatcaaaaac gatattgaaa tcttagcaga gttgactgaa ggcggaagcg
     7201 ccgttcagcg ggtggaaaca cttaagcctg atatcgtcat cattgatgtc gatatccccg
     7261 gagttaacgg tatccaggtg ttagaaacgc tgagaaagcg ccaatatagc ggaattatta
     7321 ttattgtctc cgctaaaaat gaccattttt acgggaaaca ttgtgctgat gctggcgcta
     7381 atggtttcgt gagtaaaaaa gaaggcatga acaatatcat tgcggctatt gaagctgcaa
     7441 aaaatggcta ctgctatttc cccttctctc tcaaccggtt tgttggaagt ttaacgtccg
     7501 accagcaaaa actcgactcc ttatcgaaac aagaaattag tgtcatgcgg tatattcttg
     7561 atggcaagga taataatgac attgctgaaa aaatgttcat cagcaacaaa actgtcagca
     7621 cttataaaag tcgcttgatg gaaaaattag aatgcaaatc actgatggat ctttacacat
     7681 tcgcacaacg taacaaaatc ggctaaccac atgaagtttt taccctatat ttttcttctc
     7741 tgttgtggtc tttggtcgac cataagtttc gcagacggag attacatcga atatcgtggc
     7801 atcagtagta acaaccgtgt cacacttgat ccactacgtc tgagcaacaa ggaattacgt
     7861 tggctcgcga gcaaaaaaaa tcttgtgatt gcagtacaca agtcccaaac agctacgttg
     7921 ttgcataccg attcgcagca acggattcgt ggtattaatg ctgattattt aaatctttta
     7981 aaaagagcgt taaatatcaa attaacactc cgggaatacg cagatcatca aaaagcaatg
     8041 gacgcgctgg aagacggtga agttgatata gtgttatcac atttagttgc ttcgccgcct
     8101 cttaatgatg acattgctgc aaccaatcct ctgataatta cctttccggc gctggtcact
     8161 acccttcacg attcaatgcg accgcttacc tcatcaaaac cagtaaatat tgctcgagta
     8221 gcaaattatc ccccagacga ggtaattcat caatcatttc caaaagcaac aattatctct
     8281 tttacaaatt tatatcaggc attagcatcc gtctcagccg gacagaatga ttactttatt
     8341 ggtagtaaca tcattaccag cagtatgatt tcccgttatt ttactcactc cttaaatgta
     8401 gtgaagtatt ataactcacc gcgtcaatat aactttttat taaccagaaa agattcaatc
     8461 gttcttaatg aagtactcaa tcgatttgtt gatgctttaa caaatgaagt tcgctatgaa
     8521 gtatcacaaa attggcttga tacaggaaac ctggcctttc tgaacaaacc attagaactc
     8581 actgaacatg aaaaacagtg gattaagcag catcccgatt taaaggtgct ggaaaatcct
     8641 tactcgccac cctattctat gacagatgaa actggctcag ttcggggcgt gatgggtgat
     8701 attcttaata ttattacctt gcaaacaggt ttaaattttt ctccgatcac cgtttcacac
     8761 aatattcatg ctgggacaca gcttaacccc ggtggatggg atatattgcc cgccgctatt
     8821 tatagtgaag atcgagaaaa taatgtttca tttgctgaag ccttcataac aacgccttac
     8881 gtttttgtca tgcaaaaagc gcctgacagt gaacaaacat taaaaaaagg aatgaaagtt
     8941 gccattccat attattatga gcttcattcg caattaaaag agatgtatcc ggaggtagag
     9001 tggataaaag tcgataacgc cagcgctgca tttcacaagg tcaaggaagg tgaacttgat
     9061 gctctggtcg cgacacagtt aaattcgcgt tacatgatcg accattacta tcctaatgaa
     9121 ctttatcatt ttcttattcc cggcgttcag aatgcatcac tttcgtttgc ttttcctcgc
     9181 ggagaaccgg aacttaagga tattattaat aaagcactga atgcaattcc cccaagcgaa
     9241 gttctgcgcc tgaccgaaaa atggattaaa atgcccaatg tgaccattga cacatgggac
     9301 ctctatagcg agcaatttta tattgttacg acattatccg ttttattagt tggcagtagc
     9361 cttttatggg gattctacct gttacgctca gttcgtcgtc gtaaagttat tcagggtgat
     9421 ttagaaaacc aaatatcatt ccggaaagcg ctctcggatt ccttaccgaa tccaacttat
     9481 gttgtaaact ggcaaggtaa tgtcattagt cacaatagtg catttgaaca ttatttcact
     9541 gctgattact ataaaaatgc aatgttacca ttagaaaaca gtgaatcccc ctttaaagat
     9601 gttttttcta atacgcatga agtcacagca gaaacgaaag aaaaccgaac aatatacaca
     9661 caggtatttg aaattgataa tggcatcgag aaaagatgca ttaatcactg gcatacctta
     9721 tgtaatctgc cagcaagcga acatgctgtt tatatttgtg gttggcaaga tattaccgag
     9781 acgcgtgatt taattcatgc actcgaagta gagagaaata aagcgatcaa tgcaactgtc
     9841 gcaaaaagcc agtttctggc aacaatgagt cacgaaataa gaacaccaat aagctccatt
     9901 atgggcttcc tggaacttct gtcgggttct ggtcttagca aggaacaacg ggtggaggcg
     9961 atttcacttg cctacgccac cggacaatca ctcctcggct taattggtga aatccttgat
    10021 gtcgacaaaa ttgaatcggg taactatcaa cttcaaccac aatgggtcga tatccctact
    10081 ttagtccaga acacttgtca ctctttcggt gcgattgctg caagcaaatc gatcgcatta
    10141 agttgcagca gtacgcttcc tgaacgctac ctggttaaga tcgaccctca ggcatttaag
    10201 caggtcttat ctaatttact gagtaatgct ctcaaattta ctaccgaggg ggcagtaaaa
    10261 attacgacct ccctgggtca cattgatgac aaccacgctg ttatcaaaat gacgattatg
    10321 gattctggaa gtggattatc gcaggaagaa caacaacaac tgtttaaacg ctatagccaa
    10381 acaagtgcag gtcgtcagca aacaggttct ggtttaggct taatgatctg caaagaatta
    10441 attaaaaaca tgcagggcga tttgtcatta gaaagtcatc caggcatagg aacgatattt
    10501 acgatcacaa tcccggtaga aattacccaa caagtggcgg ctgtcgaggc aaaagcagaa
    10561 caacctataa cactacctga aaagttgagc atattaatcg cggatgatca tccgaccaac
    10621 aggctattac tcaaacgcca gctaaatcta ttaggatatg atgttgatga agccactgat
    10681 ggtgtacaag cgctacacaa agtcagtatg caacattatg atctgcttat tactgacgtt
    10741 aatatgccga atatggatgg ttttgagttg actcgcaaac tccgtgagca aaattcttcc
    10801 ttacccatct gggggcttac agccaacgca caggctaacg aacgtgaaaa agggttaaat
    10861 tgcggcatga acttatgttt gttcaaaccg ttgaccctgg atgtactgaa aacacattta
    10921 agtcagttac accaggttgc gcatattgca cctcagtatc gccaccttga tatcgaggcc
    10981 ctgaaaaata atacggcgaa cgatctacaa ctgatgcagg agattctcat gactttccag
    11041 catgaaacac ataaagatct acccactgcg tttcatgcac tagaagctgg cgacaacaga
    11101 actttccatc agtgtattca tcgcatccac ggtgcggcta acatcctgaa tttgcaaaag
    11161 ttgattaata ttagccatca gttagaaata acacctgttt cagatgacag taagcctgaa
    11221 attctccagc tgctgaactc tgttaaagaa cacattgcag agctggacca ggagatagct
    11281 gttttctgtc aacaaaatga ctaaatagcg gctcccacaa tattcaaatg tgggagctat
    11341 ttaccagcac atctcacatt tatgatgaga actcctggcg aatttgttcc ccatgctggt
    11401 cgagcgttgc cgctcccggc ataacatgcg gatccgcgca gccgctgatt tttatcggat
    11461 tacctggcat cattattccc ccggcttcaa tcaacatatt tctcgcctga gtttgtggca
    11521 aattaatagc ctcagccaca cttaacaacg gcgcgacggg cacaccaact tcatgtattt
    11581 taactaacca aacttcagct gcctgcgttt ttaacgttcg ctcaatatat tgtttaagaa
    11641 tagcctggtt ttgtacgcgt aaaatattgc tgctaaatcg gggatcatta accagttccg
    11701 taagctccag tgcctggcat aacgcagaaa aaagcttgtc attaccacaa caaatagtaa
    11761 tcggcttatc ctgagtatcg aaaacatcaa aaggtgccat gtagggatgg cgatttccta
    11821 aacgttgtgg tgacttccca gtcgcgatat atgccatcag tccatgctcc agaaaactca
    11881 gagtggcatc aaacatcgct atatcgacat gcgcacctct ctggctcttt tcgcggccat
    11941 aaagtgcact cactattcca ctgaataaat aaacaccacc acagagatcc gcaagagagg
    12001 taccaacgcg cactggcgga gcatcagggt atcctgtttc catcattatc ccgctcattg
    12061 cctgaatgat ggtatcgtag gcaggagcat cttttagcgg accggtatgt ccgaaacctg
    12121 acgatgaagc atatataagg cgcgagttga tttcttgtaa cctttcccat gaaaacccga
    12181 gtttttccat tgtacctggg cgaaaattct cagctaatac atcagcttgt ttaagcatat
    12241 ttataaatat acttttatcg tgatcattct ttaaatcaag aaccacactc tctttgccat
    12301 gattaataaa actgtaatag agtgactgtc catccacata gggaccaaat gtgcgggtat
    12361 catcaccatg acccggcggc tcaactttaa ttacccttgc gcccatatta caaagaagtt
    12421 gagttccgaa aggcccatta aggacatgcg tcatatcgat aactaataag ccttcaaacg
    12481 gccctttgca ttcattattt gtcatttgct tatgccttga aaaaagccga aggtgaaaag
    12541 acacttcggc atttatgtaa aatcaataaa atgattaatt atgaaaccag tcgactgaca
    12601 taaatccaca aaggagccgt gacgacaaaa cccagtacgc tcacagccaa tgacgctgta
    12661 ccagtgcggg tgtagacatt aaaccggctg gcaataatga ttccggagaa tgccggcggt
    12721 aatgcgcctg ccagtaccat catctgcaaa tgttcgctat tcaaatgaca tgccatacct
    12781 acgagaagca gcgccagtgg catcagaatc agcttcagga aagtgttata agcaatttcg
    12841 gcactgaact cgaatttatg tgcagccaga gtcaaccccg cagcgaatac cgctacccct
    12901 gagttagcct tcgcaatcag attaaaggtt gggtcccatg ctgccggaat ttttacccca
    12961 accagcacca ggatcgttgc cagaacaggt gcccatacta ctggctcctt tgccgcagaa
    13021 attaatgcgc tcagattact attcttctta ccatccgctc ctgaggaagg attcagcaaa
    13081 tacagaccaa taggaatagt aattgcgtta acaataatag aaataattgc taccactaaa
    13141 cctgttgata cggaatcacc ataaatagga tcgagaactg caaaccccag gaatccaatg
    13201 gtaggtgaac ctgcaattaa tgcacatact gctgcttctg catgggtacg tttaaaaaat
    13261 ttgtagcaac cgaaccagga gaaaaagaaa cacccgacaa taaccacaag tgatacaagg
    13321 gtcaggcgag tgtccgcaaa aatcatttcc ctgtttgccc tggtaataga aacaaataga
    13381 gccgcaggaa gagcgtagtt taataccagt ttattaaatg cccgagcttg atcctctgaa
    13441 aaagtttctc gtctgccgct aaaataaccc aacagcatga ttacgataat aggcaataaa
    13501 tcgccaataa aaaatgttag cataatagta tttccgataa gggtcccctt accatcgcat
    13561 aataagttgg caagaagaga cgatgtcagt atttaattac cagcgacttg ttttgggtta
    13621 agtttggtaa tatggccact ttcagttcct gctgctgggt cgatgaccac attaataatg
    13681 gtcggtttgc gcgactgaat tccggtggtt aaagcatgac gaagttcatc tgtcgtggtg
    13741 acgttatagc caactccacg aaacgcatcc attaatttgt cataccttgc atggtgcaac
    13801 agatccgttg gcgatggtgc accagcgccg ctgagatcaa caccgtctcc tctgtagatg
    13861 ccgccattat taaaaataac gatcgtcacc ggcaggttat atcgacaaat cgtttcaatc
    13921 tccatcccac tgaaaccaaa agcactatca ccttcaatgg cgacaaccgg agaaccagag
    13981 gtcacgctag caccgatggc atagcccata ccgatgccca tgacacccca ggtgccacaa
    14041 tccagacgac gacgtggttt atacatatca ataatatttc gtgcattatc cagggtattt
    14101 gccccttcat taactaaata aatatcctgg ttctcgcgca aaacatcgcg cacagcactc
    14161 aatgcattaa agtaatttaa tggttgggta tctgtactta acttttcatg cattttttgt
    14221 gcattttgct gcttgtggat atttaaaata tcgcgccaca ccagtggagt cgtgaatgtg
    14281 ttttgtttca attctgccaa cataccttgc atactggatg caatatcgcc aacgactggc
    14341 acagcaatgg ggcggttgct gtcaatttcc tgcggttcaa tatccagttg aataaactgt
    14401 gtttctgccg cccatccttt tttaccgtgt gccagtaacc aattcagtcg tgcaccaaca
    14461 agcatgacaa cgtcagcatt tgccagggca aacgaacgcg cagctgccgc agagagtgga
    14521 tgcgtatctt cgaggatccc tttcgccata gacattggca ggaatggaat ctgggcactt
    14581 tcaataaatt cacgaagctg ttcatcagat tgtgaatacg ccgcgccttt gccaaggata
    14641 attaatggtc gttcagcttt tgctaaaagc gaaattgcgc tagtgactga cttcgggcat
    14701 ggcaataatg ctggcgacgg attttcaact ttaacaatcg tggttaacgc ttcgtctttt
    14761 tccatcgtcg cggccaggac atttgctggc aaatcaagat aaacaccgcc aggacgaccc
    14821 gatactgaga cccggatagc gcgtgccaat gcaatgccaa gatcctgcgg ctgattaacg
    14881 cgaaatgctg ctttggcata cggttttgcc gcattcattt ggtccagctc ttcataatca
    14941 ccttgctgga gatcgacgat cgcgcggtcg ctggaaccgc taatcatgat catcggaaaa
    15001 ccatttaccg ttgcattggc caatgcggtc aaaccattga ggaatcctgg cgcagacact
    15061 gtcaggcaga tccctggttt ttgggtaaga aaaccgcttg ctgcagcggc atagcctgcc
    15121 gactgctcat gacgaaaacc aatataacga atgccttccg cctgggcatg gcgcgccata
    15181 tccgtcacag gaatacctac aacaccataa atagtgtcaa tattattctg ttttaatgct
    15241 tcaacgatga tatgcatacc atctgtcatt tgaagttgat ctgacatgat taataacctc
    15301 gaaattttta taaattggga gccagaagcc cccgttaata tcagatggcg tggttttgct
    15361 tcattgcagc aatttcatcg tcgctataac ccagctcctg caatacagca gcggtatgtt
    15421 cacctaatag cggcgcagct ttaatatctg gcgtaaaggc agagaatttc attggacaac
    15481 caactgtcag atattttcca cgcaacggtt gttccacttc gacaacactg ccactttggc
    15541 gcaaagaggg atcaagtgaa atttctttca tacttaaaac cggtgcacaa ggaatatcaa
    15601 actgagtcaa ataggccaca gcttcatgtt tatcaatagt gacagtgtat ttttcgattt
    15661 cagcaaaaat atcgaaaata tgtggctgtc gggcatgggc tgtactgtat gccggatcgg
    15721 taatccattc tggtttgccg atggctttac aggtgttttc ccagttttgc tcctgaatag
    15781 tgaaataaat ataggcgtta ggatcggttt cccagccttt acatttcagg atccagccag
    15841 gctgaccgcc cccacccgca ttaccaccgc ggggaactgc atcaccaaat gtgccattcg
    15901 gatactgcgg gtattcttcc agataaccca atttatcgag acgctgctgg tcgcgtaatt
    15961 tcacgcggca aaggttcaac acggcatcct gcattgacat ggtgactcgt tgcccacgcc
    16021 ccgttttttc gcgatgcagc aaagcagcaa gtaaaccgat cagcaaatgc attccggtgt
    16081 tgctgtcacc caacgctgca gcgcttacca gcggcggacc gtcccaaaaa cctgtagtag
    16141 atgccgcgcc acccgctgcc tgagcaacgt tttcataggc ttttacattc acataaggcg
    16201 aacactcatc aaaccctttg atcgaaccaa aaatcagacg tggattgatt tcttgaatat
    16261 gctcccaggt gaagcccata tgatcaatgg cccctggatg aaagttctcg actaagatat
    16321 cagcttcgcg gatcagcttt tccattacct ctttgccttc cgctgttttg gtatttaatt
    16381 caatagaacg tttgttactg ttaagcatgg tgaagtaaag cgcatcgata tcaggaatat
    16441 ctcgcagctg gtggcgcgtt acgtcaccaa cgccgggacg ttcaatttta atgacgtcag
    16501 cgccaaacca ggccagcatt tgagtacaag atgggccaga ttgcacaccg gtgaaatcga
    16561 gaactttaat tccttgaagt ggagttgaca tagcaatacc tattagttaa ttaaatatgt
    16621 gttaatttag cgtttattta aggcataacg cctctataaa taatctgcaa aatatcgtcg
    16681 tagctgcact cgatattttt aaattcctgt atcaagtgct gaaaatcatt tcatcgctac
    16741 ttccaattcc tttctgcatg gtaagtagtg aggacgaaaa gaagaatata ataaatattg
    16801 gttcgcggta atcagagaga gccgcgacat ttgtgatgtt attcttacaa ctgtaagaag
    16861 tgtcagtgat ttacaattaa ggt
//
LOCUS       DAZFFE010000102        16569 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFFE010000102 DAZFFE010000000
VERSION     DAZFFE010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.102"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            115..615
                     /gene="tssB"
                     /locus_tag="ACHJ69_003708"
     CDS             115..615
                     /gene="tssB"
                     /locus_tag="ACHJ69_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO5016603.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLNLLAVGDYSNG
                     KEQRPLSERGKVDINKNNFNSVMAEFSPAVNLTVEDTLNGNGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKI
                     APQENV"
     gene            674..2212
                     /gene="tssC"
                     /locus_tag="ACHJ69_003709"
     CDS             674..2212
                     /gene="tssC"
                     /locus_tag="ACHJ69_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO5016604.1"
                     /translation="MSVQQEHATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDL
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGEDVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVRIELLDISKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVPAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWIRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDINLSLVSQMP
                     KAKA"
     gene            2230..3567
                     /gene="tssK"
                     /locus_tag="ACHJ69_003710"
     CDS             2230..3567
                     /gene="tssK"
                     /locus_tag="ACHJ69_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO5016605.1"
                     /translation="MKIHRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     ANVLFDRDALTSGKLKTQAVRLRFADGTLIDSDVSDVLPLACDLRALTNDSAIVLLAL
                     PLAHGNGGNLGQGEQTERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDESYIPPLLAFNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSTVFPPLFSLLSELLEASLPSRVIALDLASLPGNRWKADL
                     HDPRLREEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRS"
     gene            3564..4229
                     /gene="tssL"
                     /locus_tag="ACHJ69_003711"
     CDS             3564..4229
                     /gene="tssL"
                     /locus_tag="ACHJ69_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFO5016606.1"
                     /translation="MKKETDINIDVLLRDTFLTVVELRQGTTVRHGIELYRHCQRQVE
                     LVRERLKDAGFSRENVEHITYAQCALLDETVLSRSGMDDGQAIWMKNPLQSHFFNTLL
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPAAPERDQLISTLNGQVA
                     PFGVLPETAVLNVPLSTRQHPLLHSPFFWLVTLALLLAGVWWGLHHWLNVLVDELLPQ
                     SLR"
     gene            4242..5894
                     /locus_tag="ACHJ69_003712"
     CDS             4242..5894
                     /locus_tag="ACHJ69_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO5016607.1"
                     /translation="MRNTLKQAIVLWGMVLLLVLWSVFISPSGVLRWAGAAAIVLAVA
                     ALLIYRRRQAWTEMTGDAGLSSLPPETYRQPVVLVCGDMSAHLFTDSPVRQVSEGLYL
                     HVPDEEQLVAQVERLLTLRPAWASQLAVAYSIMPGIHRDVAVLAGRLRRFAHSMATVR
                     RRAGVNVPWLLWSGLSGSPLPERASSPWFICTGGEVQVATSTENAMPAQWIAQSGVQE
                     RSQRLSYLLKAESLMQWLDLNVLAELNGPEAKCPPLAMAVGLVPSLPAVDNNLWQLWI
                     TARTGLTTDIADTGTDDALPFPDALLRQLPRQSGFTPLRRACVTMLGITTVAGIAALC
                     LSATANRQLLRQVGDDLHQFYAVPAEEFITKARHLSVLKDDAVMLDGYYREGEPLRLG
                     LGLYPGERIRQPVLRAIRDWRPPEQKMEVTASLQVQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPQGRAVNRRVEISLVPRSDACQDVK"
     gene            5952..6443
                     /locus_tag="ACHJ69_003713"
     CDS             5952..6443
                     /locus_tag="ACHJ69_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO5016608.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFTFTKEIDASSPYLYKAVTTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKDPSREKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            6635..9271
                     /gene="tssH"
                     /locus_tag="ACHJ69_003714"
                     /gene_synonym="clpV"
     CDS             6635..9271
                     /gene="tssH"
                     /locus_tag="ACHJ69_003714"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO5016609.1"
                     /translation="MTGNHPAALLRRLNPYCARALDAAASLCQTRAHAEITIEHWLLK
                     LLEQGEGDITVIARRYEWDIDTLWQSLLAHLDTLPRPVRERPQLSEPLAALIRQAWLI
                     ASLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLRPLLDAQSDECPA
                     RQPQVTEPLTSALPETATADAPAKTLTEKQDDALLAVLNRFTEDVTEKARSGRIDPVF
                     GRDTEIRQMVDILSRRRKNNPILVGEPGVGKTALVEGLALRITEGNVPDSLKTVHIRT
                     LDLGLLQAGAGVKGEFEQRLKNVIDAVQKSPEPVLLFIDEAHTIIGAGNQAGGADAAN
                     LLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTACLMLRGLKA
                     RYAQHHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLT
                     QLHARLAALDIEREAIEQDSVFYPEASPERLAELTDLRDELQAEAGHLEAQYQQEKAL
                     AQQIMTLRQEGTDSSELQQQLRTHQSFAPLLALDVDARAVATVVADWTGIPLSSLLKD
                     EQSDLLSMEQSLENRVVGQSPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKT
                     ETALTLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRP
                     YSVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGREIDFRNTVILMTANLGSDHIMQL
                     LEEKPDATDADLHELLYPLLREHFQPALMARFQTVIYRPLGQEAMRAIVEMKLAQVAR
                     RLHQHYGLETEISNSLYDALTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAVHH
                     KPARLRLDWDDEDGIVLEFDEK"
     gene            9283..11841
                     /locus_tag="ACHJ69_003715"
     CDS             9283..11841
                     /locus_tag="ACHJ69_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFO5016610.1"
                     /translation="MNVQFFDHAHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQFT
                     SSDKAIAPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITGFKHLSSSQDEARYEV
                     RLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKSEYPAREQVMQ
                     YGEDDLTFVSRLLSEVGIWFRFATDARLKIEVIEFYDDQSGYERGLTLPLRHPSGLFD
                     GETEAVWGLNTAYSVVEKSVSTRDYNYREATAEMTTGQHDATGGDNTTYGEAYHYADN
                     FLQQGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIKVQGDDAPAVFR
                     KGVLITGVTTSAARDRSYELTFTAIPYSERYGYRPALIPRPVMAGALPARVTSTVKND
                     IYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAF
                     EEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEY
                     GGKSQLNLGHLVDAGKQQRGEGFELRTDLWGAVRAKKGIFISADAQDKAQGQVLDMQA
                     AIAQLENALSIAKNLSQAAEVAKAHSADLSEHESLNKALSELAEAGIVLSAPKGVGVV
                     SPRAVRLGSGESSVGLMAGTNVDVSAMKKITLTAGDSVGVFARKDGMKLYANQGKVEV
                     EAQNGGMRLTSRKDMEINSTENRVEITAKEELVLSCGGAYIRLKGGKIELGSPGNILL
                     KSVNVQKMSKARLSPAMPVLPGEGNYDLSLDLRDWEGAPISGAKYKITFESGSVLSGI
                     LDEQGYTLHTNVPPESASVEYDLPDPLPDAPWDPYASLLAAVDAEFSTNGQGAK"
     gene            11841..13304
                     /locus_tag="ACHJ69_003716"
     CDS             11841..13304
                     /locus_tag="ACHJ69_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009485629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016611.1"
                     /translation="MDDNMEMVTELQRALAFYQPPPESWYSGIQRKIEATGQWLWETL
                     QGDFNDNQSTAQVVTGTVISMIPFVDQICDVRDLIANCKKIKEGNDKNEDTTWAWVAM
                     ILTLVGLIPVAGSLIKGIFKVLFNSIRKAALSSRDIVRAIDASVSLFNKFIDLPAVQA
                     TMKWMKIYNPYTYAEKQVRELMAQLNVSVLLSKLDELMDVTGSLLEKAKSWGPESIRQ
                     PIEVIWELLVSIRSQANTMLAKALAPLNDVLEKLAARLHREGDDYYKAHTGANPHRPS
                     RLKDAEEVELLATKKPDWADIGGKEKYPQLEKASAEQKRLMDPENKEGGYPNIPDDKV
                     QTFHQMAPVEFKEGEKLYRVLDPGSSDNSFCWMREAEFKALKSKSQWRRRFAVWKSWN
                     ENGEYVVYTVPPGTTMKVWEGPAASQVREVTGKNGENIKVVLEGGSLQIVIDPTVLNL
                     DYLGKRQSTGWGYRDFSDEVDMYIGVPQLQTNIYVPK"
     gene            13346..14413
                     /locus_tag="ACHJ69_003717"
     CDS             13346..14413
                     /locus_tag="ACHJ69_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016612.1"
                     /translation="MAILHPQECWLLERIMSPEYYRRRFEGWQAFVELCERQVAEWSK
                     TIPLDVRRRPLYEQIDAVWGGRVLPNIRSTLKSVQYDFIQLQQGDLRVLQSGGNISSD
                     MKGLIDYPPDWMSPAAQKQYDRLKWRGAHYNNLIRRTSGGYWYDGELTYYYEESLHGP
                     LALPMQLPLYELDSSVYLREDDPVTVAGLYLPDIPDASAQLLYRSEHIPEAWQGRVRT
                     KYVNEAGIQEYYWESGAWAKCNWKRIRRVANRFINVPPEGFFPQGMPEELYNWPQREA
                     QYVTDRQRIAAYSGEACPHSGEWSIFVEGRQATETLEQGEQMPEWTDRKMEGEYKRGE
                     KFHVLWSLMNRHDGGSVWVEA"
     gene            14486..14866
                     /locus_tag="ACHJ69_003718"
     CDS             14486..14866
                     /locus_tag="ACHJ69_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016613.1"
                     /translation="MRISLASLFFLCCTVSDSVYADTDQTYDIFWGMLVKDKKDTIFV
                     RCDSPLLKMKIVRMADANTENIDKAYQIFNQKTKKSIYFAFIGNVTDAGSGKYIFNML
                     DVMETREGYCNLSDVLNAIDEQFR"
     gene            15197..15460
                     /locus_tag="ACHJ69_003719"
     CDS             15197..15460
                     /locus_tag="ACHJ69_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO5016614.1"
                     /translation="MKGIVVLGDKTTHGGQVISVSSSMVINGKKVALVGDKINCPVKG
                     HGINTIIEGSFDWISDGKQVVVNGCRCECGCVVISSIADALIG"
     gene            15466..16350
                     /locus_tag="ACHJ69_003720"
     CDS             15466..16350
                     /locus_tag="ACHJ69_003720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016615.1"
                     /translation="MGWKRTYTEEIDRPQDIYFHKWLFSLFILCILLLVIYYFFHEFV
                     VANNASLLAFFSPLLLWVLILSVQVIFYSRAIQDYSIWLENRLSINREWEAWGSRYVS
                     VMNSSLHLPGKVDVLFLSDDELETQYGLVNKSDDITWKQHDWYACFQTLTNHLELYKL
                     PYQLTQEFIILTDCDESMYSQIEEDFFRTVEKTNRTEGHFLLHISPSMSFGELDLWLK
                     DSEEKIYIVMVLQMEEQTSCSDAMACLMFATDDVTAKYKLTEKARIYRPMVVNSNNFN
                     SDLNILLTHKKYPKMPPG"
     gene            16377..>16569
                     /locus_tag="ACHJ69_003721"
     CDS             16377..>16569
                     /locus_tag="ACHJ69_003721"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016616.1"
                     /translation="MSSNVLQCFNDNNAQLKIDNIHLLESLCGLPGGNAVWLTAALTI
                     SVVVHKNSDYLMMSENNDDW"
BASE COUNT         4064 a         4044 c         4721 g         3740 t
ORIGIN      
        1 attattcatt tgtatatttt cctcatcatt gctttttatt taaatcatcc gataatcccc
       61 tgaatataat tatgtcaata accatcagaa aaagtggatg atgaggaaaa ggatatggcc
      121 gacagtttcc agaatgaagt tcccgccgct cgtgtaaata tcaagcttga tctgcataca
      181 ggcaatgcta aaaagaaagt tgaactcccc ctcaaccttc ttgccgtagg cgattacagt
      241 aacggaaaag agcaacgtcc gctgtccgaa cggggcaagg ttgatatcaa taaaaacaac
      301 ttcaacagcg tcatggctga gttttcgcct gcggttaatt taacagtaga agatacgcta
      361 aacggaaacg gtaatgaaca aaatatcgcg cttgaattta aaagcctgaa ggattttgaa
      421 cccgaacagg tagcaaaaaa tattccgcaa cttcgtgtat tactcgctat gcgcaattta
      481 ctacgtgacc tgaaatccaa tctgctggat aacgcaacgt ttcgccgtga actggaaaat
      541 atcctgaaag acccgacgct cagttcagaa ctgcgtgatg aactggcgaa aattgcgccg
      601 caggagaacg tttaacccga acgcgtattc aggttaaacc ggagaagaaa ggatcactga
      661 aaaggaaatg ctgatgtctg tacaacaaga acatgccacc tctgaaactg caacactcac
      721 caccactgag tccggcggcg tttatcagtc cctgttcgat aaaattaatt taaccccggt
      781 ctcttccatt caggaaatag atttatggca aaacagcgaa acgctggctg atgcctcacc
      841 cgatgagcgc gtgacagcag cgattcacgt tctgctttcc tgtctggcga aatcaggcga
      901 ggacgtggtc aagctcgaca agagcctgct ggattttcat atcgacgatc tggatcagaa
      961 aatcagtaaa cagcttgatg cggtcatgca ccaccctgaa ttccagaaag tcgagtcgct
     1021 gtggcgtggt acatggttcg tcgtacagcg cactgatttt cgcaaaaatg tcagaattga
     1081 attgctggat atcagcaaag agcatctgcg tcaggatttt gatgattcac cggaaatcat
     1141 tcagagtggt ttatatcgcc atacatacat tcaggaatat gatacgccgg gtggcgaacc
     1201 tgtagcctca ttaatatcca gctatgaatt tgataacagc ccgcaggata ttgccctgct
     1261 gcgtaatatt tccagagtgc ctgccgcttc ccatatgcct tttatcggtt ctgtcggacc
     1321 gaaattcttc cttaaaaatt cgatggaaga agtcgccgcg attaaagata tcggcaacta
     1381 ctttgaccgc gcagaatata ttaaatggaa gtcgttccgc gatacggatg acagccgcta
     1441 tgtgggatta gtgatgccgc gcgtgctggg ccgtctgccc tatgggccgg acacggtgcc
     1501 ggtacgcagc tttaactatg tggaagaagt caaaggcccg gatcacgaaa agtacctgtg
     1561 gacaaacgcc tcgttcgcct ttgccgccaa tatggtgaag agctttgtga ataatggctg
     1621 gtgtgtgcag atccgtggtc cacaggcagg tggcgcagtg gccgatctgc ccatccatct
     1681 ttacgatctc ggcaccggca atcaggtcaa aattccgtcc gaagtgatga tcccggaaac
     1741 ccgcgaattt gaatttgcca accttggctt tattccgctc tcttattata agaatcgcga
     1801 ttacgcctgc ttcttctcgg caaactccgc ccagaaaccg gcgttgtatg ataccgctga
     1861 cgccaccgcc aacagccgta ttaatgcccg tttgccctat attttcctgc tgtcccgcat
     1921 tgcgcattac ctgaaaatca tccagcgcga gaatatcggt accaccaaag accgccgcgt
     1981 actggaactg gaactgaaca cctggatccg cacactggtg acggagatga ccgatccggg
     2041 tgatgaactg caggcgtctc acccgctgcg cgacggcaag gttatcgtgg aagatattga
     2101 ggacaatccg ggcttcttcc gcgtcagact ctttgccgtg ccgcatttcc agattgaagg
     2161 gatggatatc aacctgtcac tggtttccca gatgccgaaa gcgaaagcct gatcgggcag
     2221 tggtgtgata tgaaaattca tcgaccactg tgggctgagg ggacattcct gtcctctcag
     2281 cagtttcagc agcaggcccg ctgggaagcg ttcagcaatg acagcattgc gcaactttgt
     2341 attcgtcatc cgtgggggat cgcgaatgtg ctctttgacc gcgatgccct gacgtccgga
     2401 aagctgaaaa cgcaagctgt acgcctgcgt tttgctgatg gcacgctgat cgattctgac
     2461 gtgagcgatg ttctgccgct cgcctgcgat ctgcgcgcgc tgacgaacga cagcgcgatt
     2521 gttctgctgg cgctgccgct tgcgcatggt aacggcggta atctcggtca gggtgagcag
     2581 actgaaagac cgctgcgcta tcgtcaggaa tggcaaaagg tgcaggatat ctacggcagt
     2641 gacagtgaag atatggccgt tgagcgtcat gcgctgtccc tgcgctttgc tcatgataac
     2701 aatcaggatt acatcacctg cccactggcg cgtctggtgc gggatgttca gggcaactgg
     2761 acacaggatg agagctatat tccgccgtta ctggcgttta acgcccatga cgggctggta
     2821 cagcgcctgg acacgctgct gttgcagcta cgggccaaat gtcagcgcct gatggcgatg
     2881 cgccgggaaa gcaaccagcg tatggcggac ttcgccgtcg ccgatgtctc cctgttctgg
     2941 ctgcttaacg cgctgaactc cgccgagccg gtactgagtg atttcctgcg ctacccggcg
     3001 gttcatcccg aactggtctg gcgggagctg gcgaggcttg ccggtgcgct gctgacgttt
     3061 tcccttgaac ataacgtcag cgccgttccg ccctatgtcc acgaatcgcc gtccaccgtc
     3121 tttccgcccc tgttctccct gctcagcgag ctgctggaag ccagcctgcc gtcgcgggtg
     3181 atcgcgctgg atctggcgtc cctgccgggc aaccgctgga aagccgacct gcacgatccg
     3241 cgcctgcggg aagaggccga tttttatctg tccgttcgtt cctcactgcc tgcgcatcag
     3301 gtactgcacc agctaccgct ggtctgcaaa atcggtgcgc ctgatgacgt cacgctgctt
     3361 atcaacgtgg cgctgaacgg tgttccactg gtgccactca cctcggttcc ggcggcgctg
     3421 ccgctgcggc tggagaacca gtattttgcg ctggatatgc acagtgacgc ggctaaatcg
     3481 atgctggagt ccgggtgctg catgatttat gcgccgggaa caatgggcga cctgaaacct
     3541 gaactgtttg cggtactgcg ctcatgaaaa aagagacgga tatcaatatc gacgtgctgc
     3601 tgcgcgatac gtttctgacg gtggtggaac tgcgtcaggg aaccaccgtg cgtcacggta
     3661 tcgagctgta tcgccactgt cagcggcagg tggaactggt ccgcgaacgt ctgaaagatg
     3721 ccggattcag tcgggagaac gtggagcaca taacttacgc gcagtgcgcc ctgctggacg
     3781 aaacggtact cagccgtagt ggcatggacg acggtcaggc catctggatg aaaaacccac
     3841 tccagtcgca tttcttcaac accctgctgg ccggtgagct gctgtacgag cggatgaagc
     3901 aggtgctaca ggagcccgcc cccgcgcagg ccgtgctgac ctgctttcac cgcgttctgc
     3961 tgctgggatt tcgcgggcgt tatcaggatc cggcagcccc ggagcgcgac cagcttattt
     4021 cgacgctcaa cgggcaggtc gcgccatttg gtgtgctgcc ggaaacggcg gtactgaacg
     4081 tgccgttatc cacccgtcag caccctttgc tgcattcgcc gtttttctgg ctggtaacac
     4141 tggcgctgct actggcgggc gtctggtggg ggctacacca ctggctgaat gtactggtgg
     4201 atgaactgct gccgcaaagc ctgcgttaaa gggagtcgtg agtgaggaac acgctgaaac
     4261 aggccatcgt gctgtgggga atggtgttac tgctggtgct gtggtcggtg tttatcagtc
     4321 cgtctggcgt gctgagatgg gccggtgcgg cggctatcgt tctggcggtt gctgcgttgt
     4381 tgatttaccg gcgcaggcag gcgtggacgg agatgaccgg cgatgccggg ttgtcatcgc
     4441 tgccgccgga aacctaccga cagccggtag tgctggtctg tggtgatatg tcggcgcacc
     4501 tgttcactga cagcccggtc cgccaggttt cagaagggct gtatctgcat gttcctgatg
     4561 aagaacagct tgtggcgcag gtggagcgat tgctgaccct tcgcccggcg tgggcatcgc
     4621 agcttgccgt ggcgtatagc atcatgcccg gcatacaccg ggatgtggcg gttctggccg
     4681 gacggctgcg acggtttgcc cacagtatgg cgacggtgcg tcgccgggcg ggcgtaaacg
     4741 tcccctggct tctctggagc gggctgtccg gctcgccgtt gccggaaaga gcgagttcac
     4801 cgtggtttat ctgtaccggc ggcgaagttc aggtagcaac atccacagag aacgcgatgc
     4861 ccgcgcagtg gattgcacaa tccggcgtac aggaacgcag tcagcgactc tcttatctgc
     4921 tgaaagccga aagcctgatg caatggctgg atctgaatgt gctggcggaa ctgaacggcc
     4981 cggaggcgaa atgtccacca ctggcgatgg ccgtggggct ggtcccctcg ttgcctgcgg
     5041 tggataacaa cctgtggcag ttgtggatca ccgccagaac cggcctgacg acggatatcg
     5101 cggacaccgg cacagacgat gcgctgccgt tcccggatgc cctgttacgg cagttgccgc
     5161 gtcagtcggg ctttaccccg ctgcgacggg cctgcgtgac catgctgggt attactaccg
     5221 tggcgggtat cgccgcgctg tgcctgtcag ccacggcgaa tcgccagtta ttacggcagg
     5281 tcggtgacga tctgcaccag ttttatgccg tcccggcgga agaatttatc accaaagccc
     5341 gtcacctgtc ggtgctgaaa gacgatgcgg tcatgctcga tgggtattac cgggaaggag
     5401 aaccgctgcg tctcggtctg gggttatacc ccggtgaacg catccgccag ccggtattac
     5461 gcgccattcg cgactggcgt ccgcctgagc aaaaaatgga agtgacggct tcgcttcagg
     5521 ttcagaccgt gcgtcttgac agtatgtcgc tgtttgacgt cggacaggcc cgcctgaaag
     5581 acggctcgac aaaagtgctg gtggatgcgc tggtgaacat ccgggcaaaa ccgggctggc
     5641 tgatcctcgt ggccggatat accgacgcca ccggcgatga aaaaagcaat cagcagttat
     5701 cgctgcggcg tgccgaagcg gtgcgcaact ggatgctgca gaccagcgac atcccggcga
     5761 cctgttttgc cgtacagggg ctgggcgaga gccagcctgc ggcgaccaac gacacgccac
     5821 agggccgggc agtcaaccgg cgtgtcgaaa tcagtcttgt tccgcgttct gacgcctgtc
     5881 aggacgtgaa ataaaacata ccgccggaag aaggcggtgc ttcaatcaca ctaacaagga
     5941 gagtaattct catggctatt cctgcttatc tctggctgaa agatgacggc ggcgcggata
     6001 tcaaaggttc cgtggacgtt caggggcgcg aaggtagcat cgaagtggtg gcgctggatc
     6061 acgatgtgta catcccgacc gacaataaca ccggcaaact gaccggtacc cgtactcaca
     6121 agccctttac gtttaccaaa gaaatcgatg cctccagccc gtatctctac aaagctgtga
     6181 ccaccggaca gaccctgaaa acggcagaat ttaagtttta ccgcatcaac gatgccggtc
     6241 aggaagtgga gtacttcaac atcacgcttg ataacgtcaa gctggtcaga gtcgctccgc
     6301 ttatgcacga catcaaggat ccttccagag agaagcataa ccacctggaa cgtattgagt
     6361 tccgctacga gaaaatcacc tggacttaca aagacggcaa catcattcat tccgactcgt
     6421 ggaatgagcg tccttccgcc tgataactgc ggcagacaag aaactgtctg cctttttgtt
     6481 gtgtgctttg tccagtgccg caggcaccgg tactgagcaa agcataccca caatctggtg
     6541 atacctgatg ggcctgagtg aggtaacggc tatgggcggt agcgtgcctg aacgcggatc
     6601 gcaggaatac agaacacgat aaggaaaccc ttctatgaca ggaaatcacc ccgccgcgct
     6661 gctgcgtcgc cttaacccat actgtgcacg ggcgctggac gccgccgcct cgctgtgtca
     6721 gacccgcgcc catgcggaaa taaccattga acactggctg ctgaaactgc tggagcaggg
     6781 agaaggcgat atcacggtga ttgcccgccg ctatgaatgg gatatcgaca cgctctggca
     6841 gtctctgctg gcacatctgg acaccttacc ccgcccggtc cgcgaacgtc ctcaactttc
     6901 tgaaccgctg gcagcgctta tccgacaggc gtggctgata gcgtcactgg aaggcgacga
     6961 tccacaaatc cgcagccagc atctgctgat ggcgctgaca gaaaaaccga tgctgcccgc
     7021 ctgtaatgac ctgtgggtat tgctgagtct gagccgcgtg cagcttgagc ggctgcgtcc
     7081 cctgctggat gcgcagtcgg atgaatgtcc ggcacgtcag ccacaggtca ccgaaccgct
     7141 gacctctgca ctgccggaga cggcaacggc ggacgcaccg gcaaaaacgc tgacggagaa
     7201 acaggatgac gccctgctgg cggtgcttaa ccgctttacc gaagacgtga cggaaaaagc
     7261 ccgcagcggg cgaatcgacc cggtattcgg gcgcgacacg gaaattcgcc agatggtcga
     7321 tatcctctcc cgtcgccgca aaaacaaccc gattctggtg ggagaaccgg gggtgggcaa
     7381 aaccgcgctg gtggaagggc tggcgctgcg tatcaccgaa ggcaacgtgc cggacagcct
     7441 gaaaacggtg catatccgca cactggacct cggtctgtta caggctggcg cgggcgttaa
     7501 aggtgaattt gaacagcggc tgaaaaatgt catcgatgca gtgcagaaat caccggagcc
     7561 ggtactgctg tttattgatg aagcccatac cattatcggt gcgggtaatc aggcaggcgg
     7621 cgcggatgcg gcgaacctgc tgaaaccggc actggcaagg ggcgaactgc gcaccatcgc
     7681 ggcgaccacg tggagtgaat acaaacagta ttttgagcgc gacgccgcgc tggagcgccg
     7741 cttccagatg gttaaggttg acgagccgga tgatgacacc gcctgtctga tgctgcgggg
     7801 actgaaggct cgctatgcac agcaccacgg cgtgcatatg ctggacagcg ccattcagac
     7861 cgccgtgcgc ctgtcgcgcc gctatctgac cggacgccag ctgccggaca aggcggttga
     7921 tttgctggat accgccgggg caagagtccg catgagcctt gacaccctgc cggaaccgtt
     7981 gacgcagctt catgcgcgac tggcggcact ggatattgag cgggaagcga ttgagcagga
     8041 cagcgtattt tatcccgaag ccagcccgga gcggctggcg gaactgaccg atttgcgtga
     8101 tgagctacag gcagaagccg ggcatctgga agcgcagtat cagcaggaaa aggcactggc
     8161 gcagcagatt atgacgttgc gtcaggaagg aacagacagc tctgaactgc aacagcaact
     8221 gcggacgcat cagagctttg caccgctgct ggcgctggat gtggacgccc gcgccgtcgc
     8281 cacggtggtg gcggactgga ccggcatccc actctcatcc ctgctcaagg acgagcagag
     8341 cgacctgctc agtatggaac agagtcttga aaaccgcgtt gtcgggcaaa gcccggcgct
     8401 ctgcgccatc gcacagcggc tgcgggcggc taagaccggc ctcacgccgg agaacggccc
     8461 gcagggggta ttcctgctga ccggccccag cggcaccggt aaaaccgaaa ctgcgctcac
     8521 actggccgac actctgtttg gcggtgaaaa atcccttatc accattaatc tttcggaata
     8581 tcaggaaccg cataccgttt cccagttgaa aggttcgcct ccgggctatg tcggctacgg
     8641 tcagggcggc gtgctgaccg aagcggtacg caaacgccct tacagcgtgg tgctgctcga
     8701 cgaagtggaa aaggcgcacc gcgacgtaat gaacctgttc tatcaggtat ttgaccgggg
     8761 ctttatgcgc gacggcgaag gccgggaaat tgacttccgc aacaccgtga ttctgatgac
     8821 ggctaatctg ggcagcgacc acatcatgca actgctggag gaaaaaccgg acgccacgga
     8881 cgcagacctg catgaactgc tgtatcccct gctgcgcgag cacttccagc cagcactgat
     8941 ggcgcgtttt cagacggtga tttaccgccc gctgggacag gaggcgatgc gcgccattgt
     9001 ggaaatgaaa ctggcgcagg tggcccgccg tcttcaccag cactatgggc tggaaacgga
     9061 aatcagtaac agcctgtacg acgccctgac cgccgcctgc ctgctgccgg acaccggtgc
     9121 gcgtaatatc gacagcctgc tgaaccagca aatcctgccg gtcttaagcc agcagttgct
     9181 ggcgcagcag gccgtgcatc ataagcctgc ccgactgcgg cttgactggg atgatgaaga
     9241 cgggattgtg ctggaatttg atgagaaata atggaggctc ttatgaacgt acagttcttc
     9301 gaccacgcac accacaagct caaaattcgc ggcctgcaaa gtccggtgga tgtgctgacc
     9361 tttgaaggcc gcgagcaact gagtaccccg ttccgctacg acattcagtt taccagcagc
     9421 gataaagcca tcgcgccgga gtcggtgctg atgcaggatg gcgcattcag cctgacggca
     9481 ccgccggtac agggaatgcc ggtacagact gccctgcgca ccctgcacgg cgtcattacc
     9541 ggctttaaac atctctcatc gtcacaggac gaagcccgtt acgaagtgcg ccttgaaccc
     9601 cgtatggcgc tgcttacccg cagccgccag aacgccatct accagaacca gacggtgccg
     9661 caaatcgtcg aaaaaatcct gcgcgagcgg caccagatgc gcggtcagga cttcgtgttt
     9721 aacctcaaaa gcgaataccc ggcccgcgaa caggtgatgc agtacggtga ggacgacctg
     9781 acgtttgttt cacgcctgct gtcagaggtg ggtatctggt tccgctttgc caccgacgcc
     9841 cggttgaaaa tcgaagttat cgaattttac gacgaccaga gcggctacga gcgcgggctg
     9901 acgctgccgc ttcgccatcc ttctgggctg tttgatggtg aaacggaagc cgtatggggg
     9961 ctgaacaccg cgtacagcgt ggtggaaaaa agtgtctcca cgcgggacta caactaccgt
    10021 gaagccacgg cggaaatgac cacaggccag catgacgcca ccgggggcga taacaccact
    10081 tacggcgaag cctaccacta tgccgacaac ttcctgcaac aaggcgataa agaggcagcg
    10141 gaaagcggcg cgttttatgc ccgtatccgt cacgaacgct acctgaatga acaggccatt
    10201 ctcaaaggcc agagcaccag ttcactgctg atgccggggc tggagataaa agttcagggc
    10261 gacgacgcgc ctgcggtgtt ccgcaagggc gtgctgatta ccggcgtgac gaccagcgcg
    10321 gcgcgtgacc gcagctatga gctgacgttc acggcgatac cgtactcaga gcgctacggc
    10381 taccgtccgg cgcttatccc ccgccccgta atggccggag cgctcccggc gcgggtcacc
    10441 agtacggtga agaacgacat ctacgcccat atcgacaagg acgggcgcta ccgggtcaac
    10501 ctcgactttg accgcgatac gtggaaaccg ggctatgaga gcctgtgggt gcgccagtcg
    10561 cgcccgtatg cgggagacac ctacggcctg cacctgccgc tgctggctgg aacggaagtc
    10621 agtatcgcct ttgaggaagg caacccggac cgaccttata ttgcaggcgt aaaacacgac
    10681 tcggcacaca ctgaccatgt gaccattcag aactacaagc gcaacgtgct acgcaccccg
    10741 gcgaacaaca aaatccgcct cgacgatgag cgcggcaaag agcatatcaa ggtcagtacc
    10801 gagtacggcg gcaagagcca gctaaacctc ggccatctgg tggatgccgg gaaacagcag
    10861 cgcggtgaag gctttgaact gcgtaccgat ctgtggggcg cggtgcgggc taagaaaggg
    10921 atatttatca gcgccgatgc gcaggataag gcgcagggtc aggtgctgga tatgcaggca
    10981 gcgatagcgc aactggagaa tgcgctgtcg atagcaaaaa atctgtcaca ggcggcggaa
    11041 gtggcaaagg cgcacagcgc tgatctgtcg gagcatgagt ctctgaataa agcattatca
    11101 gaactggcag aggcgggaat cgtgttgtct gccccgaagg gcgtgggggt agtgagtcct
    11161 agagcggtgc gtttgggttc cggggaaagc agtgtgggac tgatggcggg aacaaatgtg
    11221 gacgtgtcgg cgatgaagaa gataacactg acggcgggag attcagtggg agtgtttgcc
    11281 cgtaaggacg ggatgaagct gtatgcgaat cagggaaaag tggaagtgga agcacagaat
    11341 ggagggatgc gcctgacatc gcgtaaagat atggagataa acagtacgga aaaccgggtg
    11401 gagataacgg caaaagaaga gctggtgttg tcgtgcggcg gtgcgtatat ccggctaaag
    11461 ggggggaaga tagagctggg gagtcctggg aatatcctgc tgaagtcagt gaatgtgcag
    11521 aagatgagta aagccagact gtcaccagcg atgcctgttt taccggggga aggcaattac
    11581 gatttgtcgc tggatcttcg ggactgggaa ggtgcgccca tcagcggtgc gaagtacaaa
    11641 atcacctttg aatcaggttc cgttctatcg ggaatacttg atgagcaggg atatacactg
    11701 cataccaatg ttcctcctga gtccgcatct gtggaatacg atcttcctga cccgttaccg
    11761 gatgccccct gggatcctta tgcgtcgttg cttgccgctg tggatgctga attttcaacg
    11821 aacggacaag gggcaaagta atggacgaca atatggagat ggtgacggaa ctgcaacgag
    11881 cactggcttt ttaccaaccg ccaccggaaa gttggtattc aggtattcag cgaaaaattg
    11941 aggcgaccgg gcagtggctc tgggaaacct tgcaaggaga ttttaacgac aatcagagta
    12001 ccgctcaggt tgtgacggga acagtcattt ccatgattcc ctttgtcgat cagatctgcg
    12061 atgttcgcga tctgatagct aattgtaaga agataaagga aggcaacgat aaaaacgagg
    12121 acaccacctg ggcatgggta gcgatgatcc tgacactggt cggtcttatt ccagtggcag
    12181 gttctttaat taaagggatc ttcaaagtgc tgtttaattc gataagaaag gcggcgcttt
    12241 ccagtaggga tattgtacga gcgattgatg cttccgtttc cctgtttaat aaatttatcg
    12301 atttgccagc cgtgcaggcg acaatgaagt ggatgaagat ttataacccg tatacgtatg
    12361 ctgagaagca ggtcagggaa ctgatggcgc aacttaacgt atcggtgctg ttaagcaagc
    12421 tggatgagtt aatggacgtc actggttcgc tactcgaaaa ggctaaatct tgggggccgg
    12481 agtccattcg ccagccgata gaggtgatat gggaactgct ggtgtctatc cgcagccaag
    12541 ccaacaccat gcttgcaaaa gcgctggcac cgttaaatga tgtgctggaa aagctggctg
    12601 cccgtctgca ccgggaaggg gacgattatt ataaagcgca tactggtgct aatccgcatc
    12661 ggccttctcg cctgaaagat gctgaagagg tggaattgct ggctaccaaa aagccagact
    12721 gggcggatat tgggggtaaa gaaaaatatc cacaactgga aaaagccagt gcggaacaga
    12781 aaagattaat ggacccggag aataaggagg gcggctaccc gaatattcct gatgataaag
    12841 tgcagacttt tcatcagatg gctcccgttg aatttaaaga aggcgagaaa ctctaccggg
    12901 tgcttgaccc tggcagtagc gataatagtt tttgctggat gcgtgaagca gagtttaaag
    12961 cactgaagag taagtctcag tggcgacgac gctttgcggt ctggaaaagc tggaatgaaa
    13021 atggtgagta tgtagtttat accgtgccac ccggtacaac aatgaaggtt tgggaaggtc
    13081 cagctgcctc tcaggtgcgt gaagtcacag gtaagaatgg agagaacatt aaagtcgtgc
    13141 tggaaggcgg atcacttcag atagtgatag atccgaccgt tctgaatttg gactatttgg
    13201 ggaaacgtca gtctacagga tgggggtata gagatttttc tgatgaagtg gatatgtata
    13261 taggtgtgcc tcagttgcaa acaaatattt atgtacctaa ataaaaagta tcaacagaca
    13321 attaattgat aaatcaggag ataaaatggc tatattacat ccacaggagt gttggttgct
    13381 ggaaagaata atgtcgccgg aatattatcg gcgccgtttt gagggctggc aggcgtttgt
    13441 cgagctgtgc gaacggcaag tggcggagtg gagcaagacc attccgctgg atgtgcgccg
    13501 tcgtccgctg tacgagcaga ttgacgccgt ctggggcggg cgggtgttgc ccaatatccg
    13561 cagcacttta aagtcggtac agtatgactt tattcagcta cagcaggggg acctgcgggt
    13621 actgcaatcc gggggcaata ttagcagtga tatgaaagga ctgattgatt atccgccgga
    13681 ctggatgtca cccgccgcgc aaaaacagta tgacaggctg aaatggcgag gtgcacatta
    13741 taataatcta atcagacgta cctcgggcgg atactggtat gacggcgaac tgacttatta
    13801 ttatgaggaa agtctgcacg gcccgctggc cctgccgatg caattaccgt tgtatgagct
    13861 ggatagcagc gtatacctgc gtgaggatga cccggtaacg gtggctggcc tgtatctgcc
    13921 ggatatccca gatgccagcg cccaactgtt gtaccggagt gaacatattc ccgaggcttg
    13981 gcaggggcgg gtacgaacga agtatgtcaa cgaggctggg atacaagaat attactggga
    14041 aagcggggcg tgggcaaaat gcaactggaa acgtatacgg cgggtggcaa accgatttat
    14101 caatgtgccg ccggagggat ttttcccaca gggcatgccg gaggagttgt acaactggcc
    14161 acagcgcgaa gcgcagtatg taacagaccg gcagcggata gcggcctata gcggtgaagc
    14221 ttgtcctcat agcggggagt ggtcgatttt tgtagaaggt agacaagcga cggagacgct
    14281 ggagcaggga gagcagatgc cggaatggac ggacaggaaa atggaggggg agtataaaag
    14341 aggtgagaaa ttccatgtgc tgtggtcgct gatgaaccgc cacgatggcg ggtcggtgtg
    14401 ggtggaagcg tagcggtagc tggatgtaca cgtaaaaacc ggggaatggg tgattttatt
    14461 ctggtttata caggagagat gaataatgag gatatcactg gcaagcttgt tttttctttg
    14521 ctgtacagta tcagacagtg tttatgcgga tacagatcaa acatatgata ttttttgggg
    14581 aatgctggta aaggataaaa aggatactat atttgttcgc tgtgacagtc cgttattgaa
    14641 aatgaaaatt gtgagaatgg cggatgcaaa tacagaaaac attgataagg cataccagat
    14701 atttaatcag aaaacgaaaa aatcaattta ttttgctttt atcggtaatg tgactgatgc
    14761 cgggagtgga aagtatattt tcaatatgct ggacgtgatg gaaacgagag aaggctattg
    14821 taatctgtcc gatgtgctta atgcaataga tgaacagttt cgatagtttc ggataaaatt
    14881 gttattttat gctttctttt ttgatacgtt acgtcaggtc gttgttttta taaatcaaca
    14941 acctcattta ttaaataaca taaatcaaaa gcaatgccat tatggccaat gaatttcaag
    15001 tggatattaa atctgtttat tataaatttt cctgtttgtg ttgaaacacc tcttaagact
    15061 gaatgctgaa ttactatatc aagagtcaca tcaattcatt atttctgata ttaaatttaa
    15121 tgttttatta tttttataat atttaatatt aaaactgcaa tttccgtaaa attataacat
    15181 tatggaggac tcaaccatga aaggtattgt tgttctggga gataagacaa cgcatggtgg
    15241 gcaggttata tctgtgtcat cttcgatggt tatcaatgga aaaaaagttg cgcttgtagg
    15301 tgataagata aactgccctg ttaaagggca tggtattaat accatcatcg aggggagttt
    15361 tgattggatt tctgatggca aacaggtcgt cgtgaatggc tgcaggtgtg aatgtggctg
    15421 tgttgttata tccagtattg ctgatgcctt gattggatga atattatggg atggaaacgg
    15481 acgtatacag aagaaataga caggccacag gatatttact ttcataagtg gttattttct
    15541 ttatttattt tatgtatatt attactcgtt atttattatt ttttccatga atttgttgta
    15601 gctaataacg catctttgtt agcattcttt tccccattat tattgtgggt attgattctt
    15661 tcagtacagg tcatatttta ttccagagct attcaggatt attcaatttg gctggagaac
    15721 agacttagta tcaacagaga gtgggaggca tggggatcaa gatatgtttc tgttatgaat
    15781 agtagtcttc atcttcctgg aaaagtcgat gttttattct tgtctgatga tgaattagaa
    15841 actcagtatg gacttgtcaa taagtcagat gatatcacat ggaagcaaca tgattggtat
    15901 gcctgctttc agacccttac taatcatctt gaactttata aacttccata tcaacttaca
    15961 caagaattta ttattcttac tgattgtgat gaaagtatgt actctcagat tgaagaggac
    16021 tttttcagaa cagtggagaa aaccaacaga acagaggggc attttttgtt gcatatttcc
    16081 ccttcaatgt cctttggaga gcttgattta tggttaaagg attccgaaga gaaaatatat
    16141 attgtcatgg tgttgcagat ggaggagcaa acatcctgtt cggacgctat ggcttgcctt
    16201 atgtttgcaa ctgatgatgt tactgctaag tacaaactca ctgaaaaggc cagaatttat
    16261 cgcccaatgg tagtaaatag taataatttc aatagtgact tgaatatttt attgacacac
    16321 aaaaaatatc caaaaatgcc gccgggttag ttggggacag tcagcgtctg ttttctgtga
    16381 gtagtaatgt attgcagtgt tttaatgata ataatgctca attgaaaatt gataatatcc
    16441 atcttttaga aagcctttgc ggattaccag ggggaaatgc ggtctggtta acggcagcgc
    16501 tgactatttc ggtggttgtt cacaaaaatt cagattactt gatgatgtcc gaaaataatg
    16561 atgactgga
//
LOCUS       DAZFFE010000103        16186 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFFE010000103 DAZFFE010000000
VERSION     DAZFFE010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.103"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(141..458)
                     /locus_tag="ACHJ69_003722"
                     /pseudo
     CDS             complement(141..458)
                     /locus_tag="ACHJ69_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            complement(613..688)
                     /locus_tag="ACHJ69_003723"
     tRNA            complement(613..688)
                     /locus_tag="ACHJ69_003723"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(653..655),aa:Thr,seq:cgt)
     gene            complement(803..2056)
                     /gene="proA"
                     /locus_tag="ACHJ69_003724"
     CDS             complement(803..2056)
                     /gene="proA"
                     /locus_tag="ACHJ69_003724"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO5016617.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSESILN
                     ANAQDVADACANGLSEAMLDRLALTPARLKGIADDVRQVCNLADLVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(2068..3171)
                     /gene="proB"
                     /locus_tag="ACHJ69_003725"
     CDS             complement(2068..3171)
                     /gene="proB"
                     /locus_tag="ACHJ69_003725"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO5016618.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            3460..4515
                     /gene="phoE"
                     /locus_tag="ACHJ69_003726"
     CDS             3460..4515
                     /gene="phoE"
                     /locus_tag="ACHJ69_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO5016619.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASSLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(4554..4955)
                     /gene="crl"
                     /locus_tag="ACHJ69_003727"
     CDS             complement(4554..4955)
                     /gene="crl"
                     /locus_tag="ACHJ69_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO5016620.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAKVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(5013..6257)
                     /gene="frsA"
                     /locus_tag="ACHJ69_003728"
     CDS             complement(5013..6257)
                     /gene="frsA"
                     /locus_tag="ACHJ69_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO5016621.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDGLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(6349..6807)
                     /gene="gpt"
                     /locus_tag="ACHJ69_003729"
     CDS             complement(6349..6807)
                     /gene="gpt"
                     /locus_tag="ACHJ69_003729"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO5016622.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            7068..8525
                     /gene="pepD"
                     /locus_tag="ACHJ69_003730"
     CDS             7068..8525
                     /gene="pepD"
                     /locus_tag="ACHJ69_003730"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO5016623.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(8582..9196)
                     /gene="prfH"
                     /locus_tag="ACHJ69_003731"
     CDS             complement(8582..9196)
                     /gene="prfH"
                     /locus_tag="ACHJ69_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO5016624.1"
                     /translation="MILLQLSSAQGPEECCLAVRKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(9193..10332)
                     /locus_tag="ACHJ69_003732"
     CDS             complement(9193..10332)
                     /locus_tag="ACHJ69_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO5016625.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESSAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRNSLGSIGGGNHFAELQQIDQIIDAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARINRQL
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(10523..10975)
                     /gene="yafP"
                     /locus_tag="ACHJ69_003733"
     CDS             complement(10523..10975)
                     /gene="yafP"
                     /locus_tag="ACHJ69_003733"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5016626.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITRIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            complement(10985..11383)
                     /gene="yafO"
                     /locus_tag="ACHJ69_003734"
     CDS             complement(10985..11383)
                     /gene="yafO"
                     /locus_tag="ACHJ69_003734"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFO5016627.1"
                     /translation="MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDAAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(11386..11679)
                     /gene="yafN"
                     /locus_tag="ACHJ69_003735"
     CDS             complement(11386..11679)
                     /gene="yafN"
                     /locus_tag="ACHJ69_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFO5016628.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEQYLNDMTDDDFNDFKE"
     gene            complement(11731..12786)
                     /gene="dinB"
                     /locus_tag="ACHJ69_003736"
     CDS             complement(11731..12786)
                     /gene="dinB"
                     /locus_tag="ACHJ69_003736"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO5016629.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERAVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKSDLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(12857..13642)
                     /gene="lafU"
                     /locus_tag="ACHJ69_003737"
     CDS             complement(12857..13642)
                     /gene="lafU"
                     /locus_tag="ACHJ69_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO5016630.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     LPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRAR"
     gene            13614..15287
                     /locus_tag="ACHJ69_003738"
                     /pseudo
     CDS             13614..15287
                     /locus_tag="ACHJ69_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(15507..16004)
                     /gene="rayT"
                     /locus_tag="ACHJ69_003739"
     CDS             complement(15507..16004)
                     /gene="rayT"
                     /locus_tag="ACHJ69_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFO5016631.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLQNRRNQLLTTQFQTLRNVIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVAKGLYPIDWAGDVTDFSAG
                     ERIIL"
BASE COUNT         3979 a         4215 c         4009 g         3983 t
ORIGIN      
        1 aagatttaga aaagcatgtc caggagttta aggacaaatc tcatactgat tatcggaagc
       61 ttttatcatt atatgattac caagcataca atgtataatt atatacacta ttattgtgat
      121 tataatttgt tactgtgtca ctcaagggca atatctttga aggtgttgtt taactgcgtt
      181 tcccgggcaa tcttttcctc tcgcttcact tccatcagat cgattccccc agagatacct
      241 cttttagctt cttcaagttt tgcaggagca tcagctaagg tgacctcagg atacacacct
      301 agtgctaaca gcttctcttt accagctaca cgatacttga gacgccaata ttctcctcca
      361 ccaggtttta ccaggaggta cagaccacca ccatcagcca acttgtaagc cttctctttt
      421 ggcttggcag tgtctatttg acgggcattg agtttcactt gggggtatct ccactaaact
      481 gaacagcaaa tcccctcaat tgcttgtaga gtttggggta cttaaataga cgtcaaaaga
      541 ctaaaagggg cattaatata ctgattataa gaggttttta aatacttgag tagacttggg
      601 gagacgttag aatggtgccg ataagaggag tcgaacctcc gaccttcgca ttacgaatgc
      661 gctgctctac caactgagct atatcggccc tgaaaggaca tgttcacgaa cgtgaatcac
      721 ggtggacaag gttaaaacta accgggcgat gcgtcaatgg ccttgtgaat caaatggcta
      781 cttttgcatc acccggtttt atttacgcac gaatggtgta atcaccaatg ccgatccact
      841 tataagtggt cagtgcttcc agccccattg ggccgcgcgc gtggagtttt tgtgtgctta
      901 ccgcaacttc cgcccccagt ccaaactggc cgccgtcggt aaaacgcgta gaggcgttaa
      961 cgtaaacagc ggaggaatcc acttcgttaa caaaacgctg ggcgttgcgc atatcgcggg
     1021 tcaggatcgc atcggagtgt tgcgtgccgt gttcacgaat atgggcgatg gcatcgtcaa
     1081 gatcgctaac gattttgacg ttcaaatcta atgacagaaa ctcatcgtca tactcttcgg
     1141 ctttaacagc caccaccttc gcggggcctg cctgcaactg cgccagcgcg gctgcatcag
     1201 cgtgtaacgt cacgccgctt tccgccattt gtttgcttaa tgcgggcagg aagctatcag
     1261 cgatgttttt attcaccagc aacgtttcta ccgtattaca cgtgctcgga cgctgagttt
     1321 tcgcgttgac aattactttc agggcttcag cgatctctgc actttcatca acgtaaatat
     1381 ggcatacgcc tataccgcct gtgatcaccg ggattgtcga ctgctcgcgg cacagtttat
     1441 gcaaaccagc cccaccgcgc gggatcagca tgtcgatgta tttatccata cgcagcattt
     1501 cactgaccag cgcacggtca ggattatcaa ttgcctgcac ggcacccgcc ggtaagccgc
     1561 aggatttcag ggcgtcctga atcaccgcta ccgttgcagc gttagtgcga caggtttctt
     1621 tgccgccgcg caggatcacc gcgttaccgg ttttcaggca cagggaagca acatcaaccg
     1681 tcacgttcgg gcgcgcttca taaatcacgc caatcacccc cagcggtacg cgacgacgct
     1741 caagacgcag gccgctgtcc agtacgccgc catcgattac ctgccccacc agatcggcga
     1801 gattgcacac ctggcgcaca tcgtcggcaa tgcctttcag ccgtgcgggc gtcagtgcca
     1861 gacggtcaag catcgcttcg ctaaggccat tggcacacgc gtctgcaaca tcctgcgcgt
     1921 tagcgttgag gatactttcg ctttgtgctt ccagttcatc ggcgattttt tccagcacac
     1981 gatttttttc gcggctggag agttgcgcta atttatacga ggcttgcttc gcggcaatgc
     2041 ccatttgttc cagcatcggc cagctcctta acgggtaatc atgtcatcac ggtgaacggc
     2101 aaccgggccg tattcatatc ccagtattgc atcaatttct tgcgagtggt gtccggcaat
     2161 acggcgtaat gcatcgctgt tgtaacgact gacgccgtgg gcgatatcgc gaccttcgag
     2221 gttgcaaata cggatgactt caccacgcga gaaattgcca gtcacgcttt taatgccttt
     2281 cggcaacagg gagctgccgc gttccagaat ggcggcagtt gccccttcat ctaccgtgat
     2341 ttcacccgca ggcggcgcac cgaaaatcca gcgtttacgg ttttcaagcg gagtcgcctg
     2401 ggcatggaac agcgtaccga cggaaatgcc ttccatcaca tcaccaataa cgcccggctt
     2461 gctgcccgcg gcaataatgg tgtcgatacc cgcacggcaa gccacgtcag cggcctgcaa
     2521 tttggtgctc ataccgccag ttccgaggcc tgaaacgcta tcaccggcaa tcgcgcgcag
     2581 tgcgtcatca atgccgtaca catctttaat cagttctgcc tgcggattgc tgcgcgggtc
     2641 agcggtatac aaaccttttt gatcggtcag cagcaacagt ttatcggcac ccgccagaat
     2701 cgccgccagc gcagaaaggt tatcgttatc accgacctta atctctgcag tagcgacagc
     2761 atcgttctca ttaattaccg gaacgatatt gttatcgagc aacgcacgca gggtatcacg
     2821 ggcgttcagg aagcgttcac gatcttccat atcagcacgg gtcagcaaca tctgcccgac
     2881 atgaatgccg taaattgaaa acagctgttc ccacagttga atcagtcgac tctgccctac
     2941 cgccgccagc agctgttttg aagcgatagt cgctggcaat tccgggtacc ccaggtgctc
     3001 acgtccggcg gcaatcgcac ccgacgtcac aataacaatc cgatgcccgg cggcatgtaa
     3061 ctgcgcgcac tggcgaacaa gttcaacgat atgggcacgg ttcaggcggc gcgatccgcc
     3121 tgttagcaca ctggtgccga gttttaccac cagcgtctgg ctgtcactca tgattctctg
     3181 ccattcaatt ttaggaaaaa atgatatcaa acgaacgttt tagcaggact gtcgtcggtt
     3241 gccaaccatc tgcgagcaaa gcatggcgtt ttgttgcgcg ggatcagcaa gcgtagcggc
     3301 agttgtttac gcttttatta cagatttaat aaattaccac attttaagaa tattattaat
     3361 ctgtaatata tctttaacaa tctcaggtta aaaactttcc tgttttcaac gggactctcc
     3421 cgctgaatat tcgcgcgtta attaaaatca ggaatgaaaa tgaaaaagag cactctggca
     3481 ttagtggtga tgggcattgt ggcatctgca tccgtacagg ccgcagaaat atataacaaa
     3541 gacggtaata aactggatgt ctatggcaaa gttaaagcca tgcattatat gagtgataat
     3601 gacagtaaag atggcgacca gagttatatc cgttttggtt ttaaaggcga aacacaaatt
     3661 aacgatcaac tgactggtta tggtcgttgg gaagctgagt ttgccggtaa taaagcggag
     3721 agtgatactg cacagcaaaa aacgcgtctc gcttttgccg ggttgaaata taaagatttg
     3781 ggttctttcg attatggtcg taacctgggc gcgttgtatg acgtggaagc ctggaccgat
     3841 atgttcccgg aatttggtgg cgactcctcg gcgcagaccg acaactttat gaccaaacgc
     3901 gccagcagtc tggcgacgta tcggaacacc gacttcttcg gcgttatcga tggtctgaac
     3961 ttaaccctgc aatatcaagg gaaaaacgaa aaccgcgacg ttaaaaagca aaacggcgat
     4021 ggcttcggca cgtcattgac atatgacttt ggtggcagcg atttcgccat cagtggagcc
     4081 tataccaact ctgatcgcac caacgagcag aacctgcaaa gccgtggcac aggcaagcgt
     4141 gcagaagcat gggcaacagg tctgaaatac gatgccaata atatttatct ggcaactttc
     4201 tattctgaaa cacgtaaaat gacgccaatc tctggcggct ttgccaataa gacgcagaac
     4261 tttgaagcgg tcgctcaata ccagtttgac tttggtctgc gtccatcgct gggttatgtc
     4321 ttatcgaaag ggaaagatat tgagggcatc ggtgatgaag atctggtcaa ttatgtcgat
     4381 gttggtgcta cgtattattt caacaaaaat atgtcagcgt ttgttgatta taaaatcaac
     4441 caactggata gcgataacaa attgaatatt aataatgatg atattgtcgc ggttggcatg
     4501 acctatcagt tttaatggat attgccggat gtgatgcatc cggcacattt cattcacgcc
     4561 gttaacttca ccggttcatc acgaaaatca tctgccggtt ccagcttcag attcagcgtc
     4621 gccagcagct cacgcagctt ctcgtgaaat tcacgcaggg tgtgctccag tcgttcaacc
     4681 actttggcat cttttatctg aacactcgtc cagtcgcctg ctttatcgaa cagaccaaac
     4741 tggtaactgt aggtaaaacg ggattcctgc gcttccagtt ccatccacca gccccagaac
     4801 tcacgcactt ccggtgccgg tttcacgttg acgcatacag ccagacaatc gaaaaagaat
     4861 cgattatctt cgcacttacc ttcacgaata tacgggccta gtgcggtaaa ttttttgatc
     4921 aatctgctct tcgggtgtcc actcggtaac gtcattgcaa tctcctgttg tgatgcaact
     4981 gttttaccaa attggtaaaa tttagcaaat ttttaacaca aacgtttttc gatccagtct
     5041 gtgatttcct gaaggccttt gtcaaaattc cgatacaccg ggttaaatgg gatctctaac
     5101 aatttaccgt cagcagatga tgaggtgatt aagcgtgagt cctcttccgg gctgaacgga
     5161 tcgttcttcc agtagcctga taacattggc gttgggcagc gacgtcccag caatccttgc
     5221 acttttaatg aatagcgatt cagctccacg cgcaacgctt cgtcggaagc atcatgcatc
     5281 cccaaacggc tcgccagaac gtcaagatac atttccggca cctgttgctg gcacttaaaa
     5341 tcactcaaca gggtatgaac taccggacca agacaggcaa ccgctttcag acgcggcgat
     5401 tcaaggtatg ccagacgcac ggcaacgtta gcgccgaaac ggaaaccaaa ggccgcgacg
     5461 cgagtgtgat ctacccacgg tacgttaggc agcgccttta agacgtgctg atgcaacagg
     5521 ctggagtcct gggtgagctt ccattttgaa gaaaagccca ccgacggcat atcaatagtc
     5581 agcatcgcaa tgccgcgcgg cgcaaaataa cgttcataca ggctgtaata gtccgtctgc
     5641 atcgcatcca gaccaccaca cattaatact gtcgggaacg ggccatcgcc tttcggcata
     5701 tgcaaaaagc cggtgatggg cgcaccgccg ggtacggtaa actccatctg ccgcatcgtg
     5761 cccggcagac gctgagcggc ctcttcatag gcgcggtttg acaaagcctg cgcttgctcg
     5821 gccaggccat ctcctttcag atgaggatag gcagcaatgt tgtacaacgt agccgcatgc
     5881 agccagtgtc gaccgctgag ttgcggatct tcttccgcac aggctttttg ttgccacacc
     5941 atcgcctggg tagcccactc ataaatccag ttgccgccgc ggtagccaat caccgtatcg
     6001 tataaatcat cgtcggtacg ttcggcatcg ctcatcacaa tacgcgcctg gacatcgagg
     6061 atttcacgtg ggtcaatgcc gcgccagatc cacatcagac ggttaatcat gcgataccag
     6121 tgagggatgg ttttaccatc aagggccgac tgcacaggca gttgtgcgcc gtgactaaag
     6181 cggcggacta gcgtcgaggt ttcaggatgt ttaaagcggg gtttgaacag ggtttcgctc
     6241 aggtttgcct gtgtcatgga tgcagcctcc agaatactta ctggaaacta ttgtaacccg
     6301 cctgaagtta aaaagaacaa cgcccggcag tgccaggcgt tgaaaagatt agcgaccgga
     6361 gattggcggg acgaatacga cgcccatatc ccacggctgt tcgatccagg tattttgcgg
     6421 aatatcaaca acataatcat caaccagcgg acgaccggcc ggttttgcga agatggtgac
     6481 aaagtgcgct tttggataca tttcacgaat cgcaaccgca gtaccaccgg tatctaccag
     6541 gtcatcaata acgatgaagc cttcgccatc gccttctgcg cgtttcagca ctttaagctc
     6601 gcgctggttg tcgtgatcgt agctggaaat acaaacggta tcgacatgac gaatacccag
     6661 ttcacgcgcc agtaacgcac ccggtaccag accgccacgg cttacggcaa taatgccttt
     6721 ccattgttca gaaggcatca gtcggcttgc gagtttacgt gcatggatct gcaacatgtc
     6781 ccaggtgacg atgtattttt cgctcatgtg aagtgtccca gcctgtttat ctacggctta
     6841 aaaagtgttc gaggggaaaa taggttgcgc gagattatag agatctggcg cactaaaaac
     6901 cagtatttca catgagtccg cgtcttttta cgcactgcct ctccctgacg cgggataaag
     6961 tggtattctc aaacatatct cgcaagcctg tcttgtgttg acaacatttt ctgctaaccc
     7021 tgtgacctgc aatactgttt tgcgggtgat cgacaaggag acttaacgtg tctgaactgt
     7081 ctcaattatc tccacagccg ctgtgggata tttttgccaa aatctgttct attcctcacc
     7141 cgtcctatca tgaagagcaa ctcgctgaat acattgttgg ttgggcaaaa gagaaaggtt
     7201 tccatgtcga acgcgatcag gtaggtaata tcctgattcg taaacctgct accgcaggta
     7261 tggaaaatcg taaaccggtc gtcttgcagg cccacctcga tatggtgccg caaaaaaata
     7321 acgacaccgt gcatgacttc actaaagatc ctatccagcc ttatattgat ggcgaatggg
     7381 ttaaagcacg cggcaccacg ctgggtgcgg ataacggcat tggtatggcc tctgcactgg
     7441 cggttctggc tgacgaaaac gtggttcacg gcccgctgga agtgctgctg accatgaccg
     7501 aagaagccgg tatggacggt gcttttggct tacagagcaa ctggttgcag gctgatattc
     7561 tgattaacac cgactccgaa gaagaaggtg aaatctacat gggttgtgcg ggaggtatcg
     7621 acttcacctc caacctgcat ttagatcgtg aagcggttcc agctggtttt gaaaccttca
     7681 agttaacctt aaaaggtctg aaaggcggtc actccggcgg tgaaatccac gttggcctgg
     7741 gtaatgccaa caaactgttg gtgcgcttcc tggcgggtca tgcagaagaa ctggacctgc
     7801 gtcttattga tttcaacggc ggcacactgc gtaacgccat cccgcgtgaa gcctttgcga
     7861 ccattgctgt cgcagctgat aaagtcgacg ccctgaaatc tctggtggat acctatcagg
     7921 agatcctaaa aaacgaactg gcagagaaag agaaaaatct ggcattgctg ctggactctg
     7981 ttgcgaacga taaagctgcc ctgattgcga aatctcgcga tacctttatt cgtctgctga
     8041 acgccacccc gaacggtgtg atccgcaact ccgacgtggc aaaaggtgtg gtcgaaacct
     8101 ccctgaacgt cggtgtggtg accatgaccg acaataacgt agaaattcac tgcctgatcc
     8161 gttctctgat cgacagcggt aaagactacg tagtgagcat gctggattcg ctgggtaagc
     8221 tggcaggtgc gaaaaccgaa gcaaaaggcg catatcctgg ctggcagccg gacgctaact
     8281 ctccggtaat gcatctggta cgtgaaacct atcagcgtct gttcaacaaa acgccgaaca
     8341 tccagattat ccacgcgggc ctcgaatgtg gcctgttcaa aaaaccgtat ccggaaatgg
     8401 acatggtgtc tatcgggcca accatcaccg gtccacactc cccggatgag caagttcata
     8461 tcgaaagcgt tggccattac tggacactgc tgactgaact gctgaaagaa attccggcga
     8521 agtaattatt tgatttgctg ccggatggcg tttaatcgcc ttccggcagt ttcatccttc
     8581 attacccttc gataaaagcc atccctgtaa acgttcgtcg cgggttgcca cgttcaatct
     8641 ggtgatggaa cattcgccgc tgcgatttca gcgccgcgct attttcctgt tgctgttgtt
     8701 ccagcttcca ggcaatcagt aatcgcgcca gccgtttgtt agcatgctga ctgcgctctg
     8761 actgaacctt cacgctaata ccggatgcca gatgcgtggc gcgtaccgcc gagtcggttt
     8821 tattgacatg ttgaccgccc ggccccgacg aacgcagcgt ctcataacgg attgcatccg
     8881 attgttcctg ctcatcagcg gtaaaacgcc caatgcccag aaaccagttt ttgcgcccat
     8941 gatgaggccg atacggactc ggacaaatcc actgaatagt gccgcaccag ctttcgctta
     9001 gagcccatgc gttatcgcca tccagagaaa tcagcgccga acgcagtgtg tcagagtagc
     9061 gacccgtttc tgtttccagc accgttaccg cgacgtcctg tcgggtagct tctttaatca
     9121 gcctgtccag tgcttttctc actgcgagac aacattcttc cggcccctga gcagaggaga
     9181 gttgtagcaa gatcatcctt ttttccctcc actgtttttg agcgtcagca ccgggcgcag
     9241 tcgcgcgaca ggaataatta acccagccag caccagacat tgcaccacgc tttcagccga
     9301 tttataggct tgtggcgctt cttcaaagat gagttgttta tcgcgacaaa ttacccgact
     9361 gccaagttca gtccgcgaga gttgcgtcgc cgtgtatttc gctgccagac gccctttaca
     9421 ctcggtgcgc ccccatttac gccccgcccc atgcgccagc gagtggagcg ttttttcgtt
     9481 cgcaacaggt ttaaccagcc aggagtaatc gccgcgtgaa ccgggaataa tcaccagacc
     9541 gttgtcatcc ggcgtggcgc ctttacgatg caaccagccc tgttgatcgc cgatttggca
     9601 cgcgctaaca aagttatgcg ccacatccag aaccggactt ccggtggcct taacttgttg
     9661 cattatgcgc aaagcgatca gctggcgatt aatacgcgca aacgccagcg catcgtcatg
     9721 ttccgcaata taccgtagcg cgtcgtcact gccttcaggc aaaccatgat gcgaaaacga
     9781 ggcaatatgc cgctgtaaaa tagactgccc caatcccctc gagccgctat gaaccagcag
     9841 ttgcagatgc tgcgcatcca gaccagccag tgcaaacagt tccgcgtcga taatttgatc
     9901 aatctgttgc agttctgcga agtggttacc gccaccgatg gagccaagcg agttgcgcca
     9961 cggatgctgt gcaaatgctg acggcaggtt ttcctccagc cagctttctt cagcaacgtc
    10021 atccagatct gataatcgct tttcaaactt atcggcgtta tatttgcgag cgagaatatc
    10081 tgtttgccat agcgccatgc cgcagccgat atcattgccg accagtgccg ggtaaaaacg
    10141 accaacagag aagaacgctg cgccaatcgg gtagccgcgt ccgggatgta aatctggcat
    10201 ccccacaacg cgctgcatgt tgggtaaatt tgccgtggtg tgtaattgtt ggatcgctga
    10261 actttcgatc cacaggtcat cggatgcgat ggtaaatacc gcatcggata agggacgaat
    10321 atatttgccc atgtaccatt ccatttatga ataaaaatca ggaaatggca gacgaatcca
    10381 gggcaagaaa aaaagaatta atcctggaaa ggtctgcaaa gagtcattga taaaggattt
    10441 gtcatgattt acctccttac agtttgtgga ataagaaaac gcgcacatac tagacgggcg
    10501 ttattttcat tgcaagctgg atttaatgtt gcggtttata tcgcatataa aaattagtaa
    10561 accacgttcc ccggcattca acgcgctgct gcttaactgt ctgaaaacca taacgttcaa
    10621 aaaagggttt tgcggttatg ctggcgtcca ccgtaagttc ggattcagac ttaatcaatg
    10681 gttttaacaa agcgctggca actccgcgtc gggtgtattc cggttcaaca aataacatat
    10741 cgatataatg tccaatgcgg gtaataaaac caaccggttg tgcattaata actgcaaccc
    10801 gcacttgtga tttcgcgagt ttctccttcc agcgagattc gtcaatctgc gcccaggcgg
    10861 caatttgctg tggagaatag tgctgactcg ccgtcatcgt aacagctcga aggaaaatag
    10921 cgcatagttg ctgaaaatca ccaggctgat agtttcttat ttgtatgtta ttcataatat
    10981 aaattcaaaa acgcatgcga aacgcttctg ccattttccc aattttatgc atttggttgt
    11041 tatctcgagc cagtttatga ggttcaggtt tcagaatggc aatgagcaac catgcttgct
    11101 catcaaacgc gccctgacaa tataccaaat gcgctgcgtc attcgttctg ctgaattggc
    11161 gcaactgtgg cggaaatgga ttattcacat ttgccagatg aatatgagca actcgctcaa
    11221 atttgattaa tggccaggta aacgagtcgt cgtagagtgc atcacgacca aatatatctg
    11281 gcaaaacacc gtcacgctta taggaaataa aatccgccgt taacgcttca agttcctctg
    11341 ctgtaagttg caggcgaata agttttgttt tgaatacccg catccttatt ccttaaagtc
    11401 attgaaatca tcatccgtca tatcattaag atattgttca gcgcggcgtg taatttcctc
    11461 taatcttgca gcactcggac ggaagcgcgc ctttatgggc tttttctcct cttgttctgc
    11521 aagcatgtcc attaatgctt caaatgcgct ggcacttaag agatatcctg cggggcgatt
    11581 attagaaaga accgcaacag gttgatcaat aaagtattta gctgggtttt tacgtaactc
    11641 agtgatattg accgattttt cagcgagaat tcgatgcata cagtgatctc ctcataataa
    11701 tgcacaccag aatatacaaa atagtataca tcataatccc agcaccagtt gtctttccat
    11761 ttgcgggtca agcaacgtca catgcagccc caccagtcgc acaccgcgcc cgccgcggcg
    11821 ttcatcccag gttttacgcg cggtggcgat tagatcagat ttattcagcc gcggccagac
    11881 gtgttcctgg gtggtttgct gaaaatcgtc gaactttaat ttcaccccct ggcgggcaat
    11941 cagcaaatca ggttttacct ttgccaaacg acgctccagt tcagggtaaa gccgctcgat
    12001 gatcgcttca cattcagacc agtgatgaat atcttccgcc atcgtgcgtt ccacgccgac
    12061 ggattttcgc aaccgttcgc tgttaacagc gcgttcgtca atgccctgac tacgctccca
    12121 caaaatgcgg ccaaatttgc caaagcgttt aagcagcatc accagatcac acttttgcac
    12181 atcaccgcag gtccgcagcc ccatcgcttc cagttttgcc gccgagactt tgccgacgcc
    12241 gggaattttt gccagcggta aggtttgcag aaatgccgga acttctgccg gcgtaatcac
    12301 aaactggccg ttgggtttat tcatgtcgga ggcgattttg gcgagaaact tgaccggcgc
    12361 aacgcccgca gacgccgtca gttgcagctc gttgaagatc gtctggcgga tttcctgggc
    12421 gatgagggtc gcagaaccgt ggcaatggac gctatcggtg acatcgagat aggcttcatc
    12481 cagtgataac ggttcaatgc gcgaggtata gcgcgagaaa atttcgcgga tatgatttga
    12541 ggcttctttg taggcgtcaa agcgccctgg aagcaaggtg agatgcgggc ataatttgag
    12601 cgccatccct gtcggcatag cgctacgtac gccaaattta cgcgcgggat aattggcggt
    12661 gctgatcacc ccccgtcgtt cgcggctgcc gccaatagca atagggatat cgcgcagggc
    12721 gggattatcg cgcatctcca ccgctgcgaa aaagcagtcc atatccacat gaatgatttt
    12781 acgcattgct cacctctcaa tactggtaaa gtatacagtg atttcagggt ttgagaaatg
    12841 cgtaaagatt cagcatttaa cgcgctcgct gcgaaagcac ctgctgctta tccagctttt
    12901 gcaccagcgg ctgcaccact ttatccccat gctgaccaaa gtattgatac agcgtatcgg
    12961 acgcactttt ggtcagcacc ataatctcaa tgcgccggtt gcccgcgctt tgcggatttt
    13021 tggcatccag cagcatctgg tccgccattg cgcttacctg catcacttta tcttccggca
    13081 ttccagcctc ttccagcacc cgacgagccg aaagcgcgcg atcgcccgaa aggttccagt
    13141 tgttgtagat attgttttta taggccatcg catcagtatg cccggtaata ataattttgt
    13201 tatcgagcga gtcgaacacc ggcgcaagct ccaccagcag cgttttaaag aacggcataa
    13261 tcttcgcgct gccgcgttca aacatattgc ggttctggtc gtctttaatc agcacgcgta
    13321 atccctgggg aacaatctcc atctccagat tcgcttccat atgcgcatca cgggcgatgg
    13381 tattgatact ggtcgccagc tcgccaagct cggtggcgga tttcttcttc aacagcgccg
    13441 ttttctcgtt gacgtcgcgc gcttttttct ccgtctcttc cggcaccgcc acggtggcag
    13501 gttttggcag atgagacggg ctgattttat ttaacggcga caacccacca ccgttaaaaa
    13561 tcgactgccc gtgcagcgcg gcgataatgc tttcacgctc ggatttactg acgctgttga
    13621 caatcaactt tatcgtcgtc accaaagggg ccgagcgtat ttccgaggtt tctgcccgct
    13681 ttaccttaga cgcgatgccc ggtaagcaga tggcgattga cgccgatctt aacgccgggt
    13741 tgatcaatca ggcgcaggcg cagacccggc gtaaagatgt tgccagcgag gccgatttct
    13801 acggtgcgat ggacggggca tcaaaatttg tgcgcgggga cgccatcgcc gggatgatga
    13861 ttctggcgat caacctgagc gccgacgccg ccttccagca atatgtgctg atgactatcg
    13921 gcgatgggct ggtggcgcaa atcccttccc tgctgctctc caccgcggcg gcgattatcg
    13981 tcacccgcgt cagcgataac ggtgatatcg cccatgacgt gcgtaatcag ttgctggcaa
    14041 gcccgtcggt tctctacacc gccactggaa ttatgttcgt gctggcggtg gtgccgggaa
    14101 tgccgcattt cccgttcctg atgttcagcg ccctgcttgg ttttaccggc tggcggatga
    14161 gcaagcgccc gcaagcggcg gaggcggaag agaaaagcct cgaaacgcta acccgtacga
    14221 tgacagaaac cagcgagcaa caggtcagtt gggaaactat tccgctgatc gaacctatca
    14281 gtttaagcct cggctacaaa ctggtggcgc tggtggataa agcgcagggc aacccgctca
    14341 cccagcgtat tcgcggcgta cgtcaggtga tttccgacgg caacggcgtg ctgctgccgg
    14401 agatccgtat tcgggaaaac ttccgcctca agcccagcca gtacgccatt ttcatcaacg
    14461 gcattaaggc tgatgaagcg gatattccgg cagataaact gatggccctg ccctccagcg
    14521 aaacctacgg cgagatagac ggcgtgctgg ggaacgaccc ggcgtacggg atgccggtca
    14581 cctggattca gcccgcgcag aaagcgaagg cgctgaatat ggggtatcag gtgatcgaca
    14641 gcgccagcgt aattgccacc catgtgaata agattgtgcg cagctatatt cctgatttgt
    14701 ttaactatga cgatattact cagctgcata accgtttgtc gtcgatggca ccgcgactgg
    14761 cggaagattt gagcgcggcg ctcaattaca gccagttgct gaaagtgtac cgggcgctac
    14821 tgactgaagg cgtttccctg cgcgatatcg tcaccatcgc caccgtgttg gtcgccagta
    14881 gtgcggtgac taaagatcat attctgctgg cggccgatgt gcgcctggcg ctgcggcgca
    14941 gcataaccca tccgttcgtt cgcaagcagg agctgacggt gtatacgctg aataatgagc
    15001 tggaaaatct gctgaccaac gtggtgaatc aggcgcagca gggcggcaaa gtgatgcttg
    15061 atagcgtgcc agtggatccg aatatgctca accagttcca gagcacgatg cctcaggtga
    15121 aggagcagat gaaagcggcg gggaaagatc cggtgctgct ggtgccgccg cagctgcgcc
    15181 ctttgctggc gcgctacgca aggttgtttg caccagggct gcatgtgctg tcgtataacg
    15241 aagtgccgga tgagctggag ttgaagataa tgggggcgtt gatgtaggca tccggcatcg
    15301 gtgcatattg ccggatgcgg cgtgaacgcc ttatccggcc tacgggtgag cgcggttttg
    15361 tcggcatgat aagacgcgta gcggcgcatc tggcaccggt gcacaaccgc cggatgcggc
    15421 gtgaacgcct tatccggcct acgggtgagc gcggttttgt aggcatgata agacgcgtct
    15481 gcgtcgcatc aggcatcggc gcacagctat aaaatgatac gctccccagc actaaaatcc
    15541 gttacgtctc ccgcccaatc gatgggatat aaccctttcg caacatcgcg gtgaaacgtt
    15601 gagaatggcc aatcactcac ttgttttacc caaccatgtt ttactggatt tatataaata
    15661 taatcaacat gatggcgata gtctttggta ttgcggatag catgttccca aaaacgtggt
    15721 tgccagatat ttttcaatcc acaagcatgg gtaaattgct ttttaatttc ccgccagcgc
    15781 gaggaaaaat catcatcgcc ttcaggtaat gtccatatgc aatgcatatg ctccggcaaa
    15841 actacccaag cgttaatttc aaaaggtctg tctcgcttaa ctttaataat aacgttacgg
    15901 agcgtctgaa actgggtggt caaaagttgg tttcgacgat tctgtaaatt taccgtgaaa
    15961 aaccaggttc cccccttgat gtaataacga cgatattcag acataacgac tcctttttct
    16021 caacggtaag aagaaaagga aaagtcggca atgatgttgt gttaaatcgg gaaagcgtct
    16081 cgcaaaaaat gcacagattt gtaggaatga taagactcgt agcgtcgcat cagacaccgg
    16141 cgcacaaccg ccggatgcgg cgtgaacgcc ttatccggcc tacaaa
//
LOCUS       DAZFFE010000104        16172 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFFE010000104 DAZFFE010000000
VERSION     DAZFFE010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.104"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..435
                     /locus_tag="ACHJ69_003740"
     CDS             <1..435
                     /locus_tag="ACHJ69_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HFO5016632.1"
                     /translation="EEVRLAKEKQEKLRQKKLISESFEKADAPKNWNYFMRVNEKYDS
                     RRIAPDFAYDNGIFTFLGFNSGKAFPAPFAVRDGQEQTLAFNVETKGKYKIMVIHNVN
                     DKFVLRYGNSVVGVVNKSFGKVLTDQRNTSSPAVERVEVNND"
     gene            428..1537
                     /gene="virB10"
                     /locus_tag="ACHJ69_003741"
     CDS             428..1537
                     /gene="virB10"
                     /locus_tag="ACHJ69_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="HFO5016633.1"
                     /translation="MTDMKKTDDLVDDANQSKLESKPVHDIGDIRNKNNRMRSGFLFG
                     LMAVLVIGIFALKTYKNYFADDQQKASESTGDTSISQVSKIRTGLGQNFDPVENKAII
                     NPGATGSGNTVSDGHKEVPQEGFRKYLSIPVAGQGGSQTGASGSSRTSEPQEERKESK
                     ENVPGKSGMKVTAINLDPDLYIEENRLIPCALTTRFVSDVAGRISCVFTEDVWSANHH
                     TKLLEQGTKAFGRYQTGTLNHGQGRMFVMWEQLRTPDNKRIDMVNTAAAGPLGEAGID
                     GWIDSHFWERFGGSLMLSMVQDVAAAAADNAPGKDRNVDYTENSRQAMAEMAKVALEN
                     SINIPPTMYKNQGDIITIMVGEDIDFSDIYELKVK"
     gene            1539..2567
                     /gene="virB11"
                     /locus_tag="ACHJ69_003742"
     CDS             1539..2567
                     /gene="virB11"
                     /locus_tag="ACHJ69_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HFO5016634.1"
                     /translation="MNGVIPVSISNKSLDFYKNQVFQSFLDIEGLTEIAVNRPGEIWT
                     EINGDWTFHNNDSVTFDFCKRFSQTLASFRGDEIGDTKPLLSATLESGERVQVVFPPA
                     CERNTISITIRKPSTRQITHKEYVANGFYDYVQSGKKHRTYDDELLDLFRARNIAAFM
                     ELAVAAGKTIVFAGATGSGKTTYMKSLIDFIPLNTRLITIEDAEEIKFFIHKNYVHLF
                     YPSESGSDSGSIITAAKLIKSCLRMKPDRILLAEIKGGDAWDFVKVAGSGHSGSMTSI
                     HAASAKDAIIQMVTKCYQNRECQNLPFDVLRKIIMDSIDIVVHVGRDGVVRHMSDIYY
                     KGAECENF"
     gene            2554..4416
                     /gene="taxB"
                     /locus_tag="ACHJ69_003743"
     CDS             2554..4416
                     /gene="taxB"
                     /locus_tag="ACHJ69_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxB"
                     /protein_id="HFO5016635.1"
                     /translation="MKTFNKSQSVFIFAVMLVAAWFAGSFIFFVLYFSQIKNLKPLKA
                     VFRSIDAYRMDIFTDSLTLSVVTTDIRVLAFVALGAGFVVSLIIPVAALIKLNEKKEN
                     IFGDARFATINDIRESNSFTLDGDEKDGIIVGIKDKKIIRYVGAAFSAMGAGTRAGKG
                     AGIVITNLMKYWWSVIILDPKRECFNITSLIRKVILGHEVYKFDPFSPVTHRFNPLYY
                     VSMGTSEGFNQLENLSLIIYPYKTDGADAGSYLNKTAGGVFKSYAVALWFMIKNDKAG
                     LKTLDIEPVFSMSKINQLFERAEPEHLLSFMNDIRSELRGKDKTLADIGVAGLKAFIE
                     MEDKTKAQLKSNFLNGLSPFANPNVANATDGNDFDLRQVRKKRMTIYFCISGDNARLA
                     EKITNIFFQLGIQVNLEKMPTDDPEIKHDCLFLLDEFPSIGAVDYIKSKSGLIAGYKL
                     KLLIVYQVGSQLEEIYSYAGSKTLLASAPCKIVYSASDVKDARELSEAMGTRTVTIGS
                     KSKSRSRGGTSRSESENLIERPLVTTNELLTLKFSEEILAMKGENPIRCEKAFYYLHE
                     YFFGDFVKVAPELANIFPRKKGKLVMPPQKVFENEIVAKGYLAVKDVPDLDKAA"
     gene            4425..4847
                     /locus_tag="ACHJ69_003744"
     CDS             4425..4847
                     /locus_tag="ACHJ69_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="HFO5016636.1"
                     /translation="MMKKLVFLLISILAGCSSPPEPTPAQFEKANEVINPSLPYVPDF
                     HGVIKSEVSGKGWVYEITSLSGVQARTPTFYYALAHADRVVVTTHDAGLWFRIRDLLK
                     MEGATAVVEWRNEKSFLPEQVKIVFIKAQNEVKNDWKK"
     gene            4831..5136
                     /locus_tag="ACHJ69_003745"
     CDS             4831..5136
                     /locus_tag="ACHJ69_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HFO5016637.1"
                     /translation="MTGKNRVVLPVFLALGLISTNANASDPCASVLCLYGKAVGQGGG
                     SECRSAEKDFFNILKKKKGSIRWSKTFDARKAFLNQCSTADPAAISKIMSKFGRVRG"
     gene            5191..5430
                     /locus_tag="ACHJ69_003746"
     CDS             5191..5430
                     /locus_tag="ACHJ69_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016638.1"
                     /translation="MSEIRFVTLASDINNIKEVYSDDELKRLLEAVNQNCPARIVLGS
                     VEKGKEVPLKWCFRTIPERISVEEINEVKIYPELF"
     gene            5512..5946
                     /locus_tag="ACHJ69_003747"
     CDS             5512..5946
                     /locus_tag="ACHJ69_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016639.1"
                     /translation="MMNKFSYVNRMLRQVFVFSFITAPSIALFVVFMFFSFNNSIAGS
                     FLKEARGLITDTPSDKVNVCLFEKGVPQDMELTNSTTEHVLKNKCITTLVDAADWQES
                     VDGSIRYFYWSSVVIGFVVWLTFNFRALEELFYRRNRKGDVE"
     gene            5943..6458
                     /locus_tag="ACHJ69_003748"
     CDS             5943..6458
                     /locus_tag="ACHJ69_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO5016640.1"
                     /translation="MRIFLSVILFLFTAFQSANAGGIPGALAGILSTLNQEIQGKVIR
                     VLDGDTIEVLQDKKPVRIRLANIDAPEKKQAFGRWSANQLKALLAAQPVTVTYTQTDR
                     YGRIIGHVFTTNGTDASRFMVQSGAAWVYERYNVDESLPALQREAQEQKRGLWVDANP
                     VPPWEWRYKHN"
     gene            6474..6878
                     /locus_tag="ACHJ69_003749"
     CDS             6474..6878
                     /locus_tag="ACHJ69_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO5016641.1"
                     /translation="MALSPKEKKELQQRFEKQLLPDLDMSFELNGEVYKFNFDKNESG
                     DYIDSFTQAVWGGYQDAEEWEKYGKHQETELPEYPEPVEGWCDCDKCKSMIFHKEKNI
                     YVCLSCHKQTEPFLPIDWNSNDYDWLPYEDEY"
     gene            6938..7258
                     /locus_tag="ACHJ69_003750"
     CDS             6938..7258
                     /locus_tag="ACHJ69_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016642.1"
                     /translation="MNIHLFSEVLFCVWVIALIVILFIVVKYYRRVHYRLNSLSETIK
                     RTQGGVNKRISENRELLELIKNQHPEILDEYPWVSGWLDSQEKFLVALADKSGIDINK
                     SGLI"
     gene            7285..7872
                     /locus_tag="ACHJ69_003751"
     CDS             7285..7872
                     /locus_tag="ACHJ69_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6012 family protein"
                     /protein_id="HFO5016643.1"
                     /translation="MYIHIVPKLYHLMANKCSLKSISIPELDFIIDSESLSVGRPWPN
                     KCLWVGMRKGRKSVNGLVLQTDKKLRWFTTIYTWDIEDMGLIYHQVNTYIEDDNFDMV
                     SQEILLNGSFDKWSNRVHSAYENKPPARIQPKMESLLNKPGENSHDVWEEFEWGDFLL
                     SREESLLLHTIQSERLSTDCSLFKRQPSIESALVI"
     gene            7884..10037
                     /locus_tag="ACHJ69_003752"
     CDS             7884..10037
                     /locus_tag="ACHJ69_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFO5016644.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNAIVTNCFGHI
                     IESQPPENYNPEYKAWKVETLPLRLYPVKYQPVESAAKQVKTILELIRRGDVTEIVHA
                     GDPDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKAL
                     ARSVADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFY
                     YTMTGVFQRGADVIRANWKPGEFAPLTDRKLLDKAWADGTAASLAGKPATVEAAATDD
                     KKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQF
                     SEAPQVIDALKSVFPQSLDIDSSRKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERN
                     VYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGE
                     SEDNDDSAFELLCKIRTGETLTTKEVIVNEKKTTPPPLFTEASLLAALVRVADFVTDP
                     TIKKLLKDKDKDKKDEHGGIGTPATRAAILETLKKRNYITLEKGKLIPTDTGYALIDA
                     LPGIAVNPDMTALWAEKQTAIENGDLTVEEFINELYDELTGMISDVDLGEMKIEPVAP
                     AGQSQRLSSPCPTCGKQIIIRQKGYFCTGCEFKIWSEFSGKKITQAQAEKLVKSGKTD
                     LIKGFKKKSGGTYDTVLVLEDKKTGKLGFPARAKK"
     gene            10034..10240
                     /gene="hha"
                     /locus_tag="ACHJ69_003753"
     CDS             10034..10240
                     /gene="hha"
                     /locus_tag="ACHJ69_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO5016645.1"
                     /translation="MKTKQEWLFQLRKCTSRDTLEKVIEINRYKLPLSESEAFYSAAD
                     HRRAELVMNKLYDKVPSGVWKYVH"
     gene            10258..10722
                     /locus_tag="ACHJ69_003754"
     CDS             10258..10722
                     /locus_tag="ACHJ69_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFO5016646.1"
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREVDFEQLLEMQEKLNV
                     VVEERREDAEREAAERQELEAKRQQAIEYIISLGLDPESLLAPVTADTVKTRRKAKGG
                     VRKAKYRFKDENGEIREWSGNGKRPLALQKLLDEGHFMEDFLIDKLQPGQVE"
     gene            10826..11482
                     /locus_tag="ACHJ69_003755"
     CDS             10826..11482
                     /locus_tag="ACHJ69_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001478769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016647.1"
                     /translation="MMIKSKKVVVIFVALSSFLLAGCDSPKDANKQNFEKAINTRLEK
                     ECITISPLGMFDSKPYPVQVAVAQANRYTSQERADEINSRQFSALDVLVKADLLTVKD
                     TLVDDVIGFTKTGKKVPGREYALTDEGKKYLKSPERPDFCVGHYKVDEIVDFTEPGDA
                     MGMKITQVNYTFSPTSIAEWAKRDDVRAAFLGLESDLKEKQTKRITLVLKNDGWSAER
                     "
     gene            12038..12862
                     /locus_tag="ACHJ69_003756"
     CDS             12038..12862
                     /locus_tag="ACHJ69_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5016648.1"
                     /translation="MSHLPASIFQISRSDAWNLTLSPDAARNMRDLAAQIGAGMPKYL
                     IAPEVSLLLSYIDDLNIRMYFETLWNTGARLNEALALKPADFMLTPTRQYPQPVVILK
                     TLKQRLKEAERRPGRPTTRHPPLHPDPRYRKPQEPAVRLVPVLDAGYALRMREYLATW
                     KKRIKHQPVWDITSRQTPLNWINTAVARAERDGVTFSVPVTPHTFRHSYAMHLTMSGV
                     PPRVLQSLLGHRYARSTEIYTRVFSLDVLTGSGLSFTCDPRRAKQLLGYPERINDE"
     gene            12967..13626
                     /locus_tag="ACHJ69_003757"
     CDS             12967..13626
                     /locus_tag="ACHJ69_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HFO5016649.1"
                     /translation="MGKIITVAGHKGGIGKSTVLCSLCVCAIRKGKTACFLETDSQGS
                     IKDFIEERKTNERLSEIPYFECYTDIPAMARKLAARFDYVFVDTPGMKSPAFVKALSC
                     ADILFTFIEPGAGIEINTLGRLVFDIKTAQAGVNPSMKAWIVLNKCSTNPSDSEASEL
                     RRQLNDDPDWLPVPRQRIYMRTAHKKAYNSGMGVHEYDDKRGNKARGEIELLLKETDV
                     L"
     gene            13720..14022
                     /locus_tag="ACHJ69_003758"
     CDS             13720..14022
                     /locus_tag="ACHJ69_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016650.1"
                     /translation="MAKLPKKIDQEIKPLDLRQFVSSAAQKPASASAVTRINLTMTDD
                     DLDTAQVFQNKYGASRAEVIRAALAALRLVSAEERRRLFDDIRKNSPKAGRPTVNK"
     gene            14058..14309
                     /locus_tag="ACHJ69_003759"
     CDS             14058..14309
                     /locus_tag="ACHJ69_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001482241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016651.1"
                     /translation="MLMKAIKSVFNFYMLYLQPFIGVVLAGYGVWKFSGDSISALLEP
                     ASYFVIGTVVLFVWYIAWQKERSKEEEFKSSIKPEDFVK"
     gene            14371..14586
                     /locus_tag="ACHJ69_003760"
     CDS             14371..14586
                     /locus_tag="ACHJ69_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016652.1"
                     /translation="MEHCIVDLGIINYHPVITWTVPVAFFFLVVLAGIVFNIGRKSNL
                     IVFILAMLLPVTTVFIFMLLGGKIDMC"
     gene            complement(14893..15420)
                     /locus_tag="ACHJ69_003761"
     CDS             complement(14893..15420)
                     /locus_tag="ACHJ69_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFO5016653.1"
                     /translation="MIFNRNKKRDEQHNSAVVLPSAENVFSDFIKNNPALTTNAFTAI
                     GEVDCFTKVPLLSNNESEFLGILSRNIIPDYSVFPKIRLIEFVRPHGSEEAVKDLIRE
                     VQNITCDFVLKSYDETVLAVIQFGETATVRQQKKKLIIQRVCAMTGISFFEFKNTVDM
                     MGSEDFCQFLRDDEE"
     gene            complement(15555..15959)
                     /locus_tag="ACHJ69_003762"
     CDS             complement(15555..15959)
                     /locus_tag="ACHJ69_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016654.1"
                     /translation="MKDNIVDFQRIRDSMNDHSTDVANDAVYSDKKKISRFFYLLTPV
                     RYLLAASLYLVVVIPLGILAGFRFLLQFLGGIAVMGLTIAWYMGADVSSYVVLAAWIA
                     LTVCGCAEQIFEWWIESRFAFRLFGIGRTPEE"
     gene            complement(15972..>16172)
                     /locus_tag="ACHJ69_003763"
     CDS             complement(15972..>16172)
                     /locus_tag="ACHJ69_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO5016655.1"
                     /translation="KRDVLNKSVKEIMKHTEVKNLSFVVSEKIGRKVYKLKFSYTIGY
                     EGDTREDSEFTNMFDKMYPPEN"
BASE COUNT         4917 a         3096 c         3648 g         4511 t
ORIGIN      
        1 gaagaagtaa ggctggcaaa agaaaaacag gaaaaactca gacagaagaa actgatttct
       61 gaaagttttg aaaaagccga tgcgcctaaa aactggaatt atttcatgcg agtcaatgaa
      121 aaatatgaca gccgtcgtat cgcccctgat tttgcttatg ataatggtat atttaccttt
      181 ttaggtttta actcagggaa agcattccca gctccatttg ctgtaagaga tggtcaggaa
      241 cagacacttg cttttaatgt ggaaacaaaa ggcaagtata aaattatggt tatccacaac
      301 gttaatgata aatttgtttt acgctatgga aacagcgttg ttggtgttgt gaataaatct
      361 ttcggaaaag tattaaccga ccagaggaat acatcttctc ctgcggtaga acgtgttgag
      421 gtgaataatg actgatatga aaaaaacaga tgatttagtt gatgatgcga atcagtcaaa
      481 gctggaaagt aaacccgttc atgatatcgg tgatatcagg aacaaaaata atcgtatgcg
      541 ttctggcttt ctgtttggtc tgatggctgt attagttatt ggtatttttg cgctgaaaac
      601 atataaaaat tattttgcag atgaccagca gaaagcatca gaatcaacag gcgataccag
      661 catttctcag gtaagtaaaa tccgtactgg tcttggtcag aattttgacc ctgttgagaa
      721 caaagcaatt atcaatccag gcgctactgg ttctggtaat actgtttctg acggtcataa
      781 agaagtgcca caggaggggt tcaggaaata tttgtcaata cctgttgctg gtcagggagg
      841 gagtcagact ggcgcgagtg gcagcagcag gacatcagag ccacaggaag aaaggaaaga
      901 atcaaaagaa aatgtgcctg gtaaatcagg aatgaaagtg actgcgatta atctcgatcc
      961 tgatctctat attgaggaaa acaggcttat accgtgtgct ttaaccacac gctttgtttc
     1021 tgatgttgca ggtcgtatca gttgtgtttt tacagaagat gtatggagtg ctaatcacca
     1081 cacaaaatta ctggaacagg gaactaaggc ttttggtcgt taccagaccg gaacgctgaa
     1141 tcatggacag ggcagaatgt tcgttatgtg ggagcagttg cggacgcctg ataataaacg
     1201 aatcgatatg gtaaatacag cagcggctgg ccccctggga gaagcgggca ttgatggatg
     1261 gatcgattct cacttctggg agcgttttgg tggttctctt atgctcagta tggttcagga
     1321 tgtagcggct gcggcggcgg ataatgcacc tggtaaagat cgtaatgtgg attataccga
     1381 aaactcacga caggcaatgg ctgaaatggc taaagtcgca cttgagaatt cgataaatat
     1441 tcctccaaca atgtataaaa accagggtga cataatcact attatggtcg gggaagacat
     1501 tgatttttct gatatctatg agttaaaggt aaaatagtat gaatggcgtt attcctgtca
     1561 gcattagcaa taaatcgctc gatttttata aaaatcaagt attccagtct tttcttgata
     1621 ttgaagggct aacagaaatt gctgtcaacc gtcctggtga aatctggacg gaaattaatg
     1681 gtgactggac attccataat aatgacagtg tgacctttga tttttgtaaa cgtttttctc
     1741 agacgctggc atcatttcgg ggagatgaaa tcggcgatac caagccatta ctttcagcaa
     1801 cactggaatc aggagaacgt gtacaggtcg tttttcctcc tgcatgtgag agaaatacta
     1861 tttcaatcac catacgtaaa ccatcgacac ggcagataac gcataaagaa tatgtggcga
     1921 acggttttta tgattatgtc cagtcaggaa aaaaacacag gacatacgat gatgagcttc
     1981 ttgatttatt cagagcgaga aatatcgctg cttttatgga gcttgccgta gcagcaggga
     2041 aaaccattgt atttgccgga gcaacgggtt ccggtaaaac aacatatatg aagagtctta
     2101 tcgatttcat accattgaat acacggctga ttacgattga agatgcagag gaaatcaaat
     2161 tttttatcca taaaaattat gtgcatcttt tttacccttc ggaatccggc agtgattccg
     2221 gctctatcat aacagcagcg aaattaatca aatcctgttt gaggatgaaa cctgacagaa
     2281 ttttactggc ggaaattaaa ggtggcgatg cctgggattt tgtgaaagtt gctggttctg
     2341 gtcacagtgg aagcatgacg tccattcacg cggcctcggc aaaagatgcc atcattcaga
     2401 tggtgacaaa atgttatcag aacagagaat gtcagaatct tccttttgat gttcttcgta
     2461 aaatcattat ggacagtatt gatattgttg ttcatgtagg cagggatggt gttgtcaggc
     2521 atatgagcga catctattac aaaggggctg aatgtgaaaa cttttaataa gtcgcaatct
     2581 gtttttatat tcgctgttat gcttgttgcg gcatggtttg caggttcatt tattttcttt
     2641 gtgctttact tcagccagat aaagaatctg aaaccattga aggctgtgtt cagatccatt
     2701 gatgcgtaca ggatggatat ttttacagac agccttactt tatctgttgt gactacggat
     2761 atcagagttt tagcctttgt tgcacttgga gcaggatttg tcgtgtctct tatcatccct
     2821 gttgcggcgt tgataaaact gaacgagaag aaagaaaata ttttcggtga tgcgcgattt
     2881 gcaacaatta atgatattcg tgagtctaac agtttcacgc ttgacggtga cgaaaaagac
     2941 gggattatcg ttggtataaa agacaagaaa ataatacgct atgtgggagc agcattcagt
     3001 gctatgggag ccggaacaag ggctggtaaa ggtgctggta tagtcatcac taatcttatg
     3061 aagtactggt ggagtgttat tattctcgat ccaaaaaggg agtgttttaa tatcaccagt
     3121 cttatcagga aggtgatttt aggtcatgag gtttataaat ttgatccctt tagtccggta
     3181 acgcaccgct ttaacccttt gtattatgtc agcatgggaa cgagtgaagg ttttaaccag
     3241 cttgaaaatc tttcgttgat tatttacccc tataagacag atggtgcgga tgctggtagc
     3301 tatcttaata agacggcggg tggtgttttt aaatcttatg ctgtcgcttt atggtttatg
     3361 attaagaatg ataaggctgg actaaaaacg cttgatattg agcctgtctt ctcaatgtct
     3421 aaaattaatc agctttttga gcgtgcagaa cccgaacatc ttctttcatt catgaatgat
     3481 attcgttcag aattaagggg gaaagacaaa acgcttgctg atattggtgt cgctggcctt
     3541 aaggcattca ttgagatgga ggataaaaca aaagcacaac tcaaaagcaa ctttcttaat
     3601 ggattatcac cgtttgctaa ccccaatgtg gcgaatgcta ctgatggaaa tgattttgat
     3661 ttgcggcagg tgcggaaaaa acggatgaca atttatttct gtatttctgg tgataatgcc
     3721 aggttggctg aaaaaataac aaatattttc tttcagcttg gtatacaggt gaatcttgaa
     3781 aagatgccaa cggatgatcc ggaaataaag catgactgcc tttttctact ggatgaattc
     3841 ccgtcaattg gtgctgtaga ttacatcaag agtaaatctg gtcttattgc tggttacaag
     3901 ctgaaattgt tgattgttta ccaggtcggc tctcagcttg aggaaatcta cagttatgca
     3961 ggcagtaaaa cattactggc aagtgcaccc tgcaaaatcg tttattctgc ttctgatgtt
     4021 aaagatgcac gggagttatc agaggcgatg ggaacacgaa cagtaactat cggttcgaaa
     4081 agcaaaagtc gtagtcgtgg aggaacatcg agatctgaat cagaaaacct tattgagcgt
     4141 ccactggtaa caacaaatga actactgaca ctgaaattta gtgaagaaat cctggcaatg
     4201 aagggagaaa acccgattcg ttgtgaaaag gcattctact atctccatga gtatttcttt
     4261 ggtgattttg tgaaggttgc tccggaactt gcaaatattt tccccaggaa gaaaggaaag
     4321 ctggttatgc ctccacagaa agtatttgaa aatgaaatcg tcgcaaaagg gtatctggca
     4381 gtaaaagatg ttcctgatct cgataaagcg gcataaagga gaacatgatg aagaagttag
     4441 tgtttttatt gatttctatc cttgctggtt gttcatcacc gccagagcca acacccgcgc
     4501 agtttgaaaa ggcaaatgaa gtgatcaatc cctcattgcc gtatgtcccc gattttcacg
     4561 gcgtcataaa gtcagaagtt agcggtaaag gatgggttta tgagatcact tccttatccg
     4621 gtgtacaggc cagaacgccg acgttctatt acgctctggc tcatgcggac agggtggtag
     4681 tgacaacaca tgatgctggg ctgtggtttc gaatcagaga tctgttgaag atggaagggg
     4741 caacagcagt cgtggaatgg cgaaacgaga aaagtttttt acctgaacag gtaaaaatag
     4801 ttttcattaa agcacaaaat gaggtgaaaa atgactggaa aaaatagagt ggttttacct
     4861 gtctttctgg cgttaggatt aatatcaaca aatgctaatg catctgaccc ttgcgcttcg
     4921 gtactttgct tatatggaaa agcagtcggg caaggtggag ggagtgagtg cagaagtgct
     4981 gaaaaggatt tttttaatat tctgaaaaag aaaaaaggaa gcattcgctg gagtaaaact
     5041 tttgatgccc gtaaagcatt tctgaatcag tgttcaacgg ctgatcctgc tgctatatcg
     5101 aaaatcatga gtaagtttgg tcgtgtcaga ggttaattaa aacctgatac ccgcgccaac
     5161 gggtatcaag ggatatacag gagaaaaatt atgagcgaaa tccgatttgt aactctggca
     5221 tccgacatta acaatattaa agaagtttat tctgatgatg agttaaaacg tttgctggaa
     5281 gctgttaatc agaattgccc agccagaatt gtgttgggta gtgtggagaa ggggaaagaa
     5341 gtaccactca agtggtgttt taggacaata ccagagcgta tttcagttga agaaatcaat
     5401 gaggttaaaa tttatccaga gttattctga actgaaataa aggcaaacgc cgcagattcc
     5461 agattttaat ctggaatctg cggggttgaa caaccgaagg gcgttagttt tatgatgaat
     5521 aaatttagtt atgtcaatcg tatgcttcgg caagtttttg tttttagttt tatcacggcc
     5581 ccatcaatag ctttatttgt tgtttttatg tttttttcgt ttaataactc aattgctggt
     5641 tcatttttaa aagaggccag ggggcttata accgatactc cttctgataa ggttaacgtc
     5701 tgtcttttcg aaaagggcgt accacaggat atggagttga caaactccac aactgaacat
     5761 gtattaaaaa ataaatgtat aacaacgtta gttgatgctg ctgactggca ggaatctgtt
     5821 gacggttcca ttcgatattt ttattggtcg tcagttgtta ttggctttgt ggtctggctt
     5881 acctttaatt ttcgtgcact ggaagaacta ttttatcgta gaaacagaaa aggagacgtt
     5941 gaatgaggat ttttttaagc gtaattttat tcctgtttac tgcttttcag tctgcaaatg
     6001 caggtggaat acctggtgct ttagcaggaa tactttctac attaaaccag gaaatccagg
     6061 ggaaagttat tcgtgttctc gatggcgata caatcgaagt tttacaggac aaaaagcccg
     6121 tcaggattcg cctggcaaac atcgatgcac cggagaaaaa acaggctttc gggcgctggt
     6181 cagcaaatca gcttaaagct ctgcttgcag cacagcctgt tacggtgacg tacacgcaga
     6241 cagaccgcta cggacgcatt atcggtcatg tgttcacgac gaacggcacg gatgccagcc
     6301 gctttatggt tcagtctggc gctgcatggg tatatgaacg ttacaacgtg gatgagtcat
     6361 taccggctct acaacgggaa gcgcaggaac aaaaacgcgg tttgtgggtc gatgcaaatc
     6421 cggttccacc gtgggagtgg cgatacaaac ataactaact gatagaggtt tttatggctt
     6481 tatcacccaa agaaaagaaa gagttacaac aacgcttcga aaagcaatta ctacctgacc
     6541 ttgatatgtc atttgaacta aatggcgaag tatataaatt taattttgat aaaaatgagt
     6601 caggtgacta tatcgatagt tttacacaag cagtatgggg aggttatcag gatgccgagg
     6661 aatgggaaaa atatggtaaa catcaggaga cagaattacc tgaataccct gaaccagtcg
     6721 agggttggtg cgactgtgat aaatgtaagt cgatgatttt tcataaagag aaaaatattt
     6781 atgtttgcct ctcctgccat aaacaaacag agccatttct tccaattgac tggaactcaa
     6841 acgattacga ttggctacct tatgaagatg aatactaatg ctgaatactt cacagtcata
     6901 atggatgaaa tggcggcata aaattaagga ggcttaaatg aatatccatt tattttcaga
     6961 agtattgttc tgcgtatggg ttatagcttt gattgtgatt ttatttatag ttgttaaata
     7021 ttacaggcgt gtccattaca gactcaattc actgtctgaa acgataaagc gcacacaggg
     7081 cggtgttaat aagcgaatat ctgaaaacag agaactgctt gaacttataa agaatcaaca
     7141 tcctgaaatt cttgatgaat atccgtgggt aagtggttgg cttgattcac aggaaaagtt
     7201 tttggtcgct cttgctgata agtctggtat cgatatcaac aaatcaggac ttatttgagc
     7261 acactaaaga taaaggaaat tactatgtat atacatattg ttcctaaact ttatcatctc
     7321 atggctaata aatgtagcct taaatctatt tccatacctg agcttgattt tataattgat
     7381 agtgaatcat tgtctgtagg cagaccgtgg cctaataaat gtttatgggt tggaatgcga
     7441 aaaggaagga agtcagttaa tggcctcgtt ttgcaaacag ataaaaaatt aagatggttt
     7501 actactatat acacctggga tattgaagat atggggttaa tttaccatca agtcaatacc
     7561 tatatagaag atgataattt tgatatggtt tcacaggaaa ttcttttaaa tggttctttt
     7621 gataagtgga gtaacagggt tcattcagct tatgaaaata agcctccagc cagaatccag
     7681 cccaaaatgg agtccttatt aaataaacct ggtgaaaaca gtcatgatgt gtgggaagag
     7741 ttcgaatggg gtgacttcct tctgtccaga gaagaaagcc ttttacttca cactattcag
     7801 tctgaacgat tgagtacaga ttgctcttta ttcaaaagac aaccgtctat tgaaagtgca
     7861 ttagttattt aaaggataat aagatgagac tttttatcgc agaaaaaccc gcagtagcaa
     7921 atgatattgt taaggcactt ggtggcaatt ttacccgcca tgatggctgg tttgaaagtg
     7981 ataacgccat tgtgactaac tgttttggtc atattatcga atcacaaccg ccggaaaact
     8041 ataatcctga atacaaagcc tggaaggttg aaacgcttcc tttacgtctt tatcccgtga
     8101 agtatcagcc tgttgaaagt gccgcaaaac aggttaaaac gattctcgaa cttatcagac
     8161 gtggagacgt gactgaaatt gttcacgctg gcgatcctga tgatgaggga cagttacttg
     8221 ttgatgaagt cctggaatat gcaggcaaca caaaacccgt aaagcgcgtt ctgattaacg
     8281 acaacacgct tccggcagtg aaaaaggcac tggcaaatct taaagataat cgtgatttca
     8341 aagggcttta ccttaaggcg ctggcgcgtt cagttgctga tgccgtctat ggcttctcca
     8401 tgacgcgtgc ttacaccatt cctgcaaaag ccagaggata tcagggcgtt ctgtctgtcg
     8461 ggcgcgtcca gactcccgtt cttggcctga ttgtgaatcg tacccgtgct aaccagaacc
     8521 ataaatccag tttttactac accatgaccg gagtctttca gcgtggtgct gatgttatca
     8581 gggcgaactg gaagccaggg gaatttgctc cgctgacaga ccgtaaatta cttgataaag
     8641 cgtgggcaga cggaacggca gcctcccttg caggaaaacc ggctacagtt gaagcggcag
     8701 caactgatga taaaaaaacg gctgcaccgt tgccctttaa cctggtacga ctccagcaat
     8761 acatgaacaa aaagttcaaa atgacggcac aaaaaacgct ggatattacg caacaattac
     8821 gtgaaaaata taaagcgatt acttataacc gctctgattg ctcatattta tctgatgaac
     8881 aattcagcga agcgccgcag gttatcgatg ccctgaaatc agtctttcct cagtcgctgg
     8941 atattgattc ttcacgtaaa agcaaagcgt ttaacagtgc aaaggtgact gcgcatactg
     9001 ctataatccc gaccgccagt gtgcctgatg ttaacgcact cagcaccgac gagcgcaatg
     9061 tttacctggc gatcgcacaa cactatcttg ttcagttcat gcctgaaaaa gcataccagg
     9121 aagtatcggt tgccattcag tgtggtgatg agtcgttcta tgcccgtgcc agaaaaacaa
     9181 ctgacagcgg atttgaggcg tttcttggcg cggaaaccac agacgaaggt gaatcagaag
     9241 ataatgatga ttccgctttt gaactgctct gtaaaattcg cacaggagaa acactgacga
     9301 caaaagaagt tattgttaat gagaagaaaa caacaccgcc gccgttattc accgaagcct
     9361 ccttgcttgc tgcgcttgtt cgtgtcgcgg attttgtcac tgacccaacg attaaaaaat
     9421 tgttgaagga taaggataaa gacaaaaaag atgaacatgg tggcattggt acgccagcta
     9481 cccgcgcagc cattctggaa acgctgaaga aaagaaacta tatcacgctg gaaaaaggga
     9541 aacttattcc tactgatacc ggatatgcgc ttattgatgc cctgcctggt atagcggtta
     9601 atcctgatat gacagcatta tgggctgaaa agcagacagc cattgaaaat ggcgacctga
     9661 ccgttgaaga gttcattaat gaactatacg atgaactcac aggcatgatt tctgatgttg
     9721 acctgggcga gatgaaaatt gaacccgtag cgccagcagg acaatctcaa cgcctgagtt
     9781 ctccctgccc tacatgcggc aaacaaatta ttatcaggca aaaaggttat ttctgtaccg
     9841 gatgtgaatt taaaatctgg agtgagtttt ctggtaagaa aatcacccag gcacaggccg
     9901 aaaaactggt taaatcaggg aaaaccgatt tgattaaggg atttaaaaag aaaagtggtg
     9961 gaacgtatga tacagttctt gtccttgagg ataagaaaac agggaagctg ggttttccgg
    10021 caagggctaa gaagtgaaaa caaagcagga atggcttttt cagttaagaa aatgtacatc
    10081 aagagatact cttgaaaaag ttattgagat taaccgttac aaactgcctt tatcagaatc
    10141 agaggcattt tattctgccg cagatcatcg ccgagcagaa ctggtgatga ataaacttta
    10201 tgataaggtg ccttccggcg tctggaagta cgtacactaa ataagaggat taattatatg
    10261 agcgaactga ctaaagaaga tgaatacggc attatcagcc gtactatgat gaatattcgt
    10321 tcattacgtg tttttgcccg tgaagttgat tttgagcaat tgctcgaaat gcaggaaaaa
    10381 ctgaacgttg ttgttgaaga acgtcgtgaa gatgctgaac gtgaagcggc tgaacgccag
    10441 gaacttgaag ccaaacgtca gcaggccatt gaatacatca tatctttagg tcttgatccc
    10501 gaatcactcc ttgctcctgt aacagcagat acagtaaaaa ccaggcggaa agccaaaggc
    10561 ggagtaagaa aggctaaata ccgctttaag gatgaaaatg gcgagatcag agaatggtct
    10621 ggtaatggca agcgtccgct tgctttacag aagctgcttg atgagggcca tttcatggaa
    10681 gatttcctca ttgacaaact gcaacctggg caggttgaat aaaataatcg tgatgccgtc
    10741 gcaaacttgt ttgcgacggc aaatggaggg gacagaacag ccagctgcgc tggcaagaaa
    10801 ttttatcgtt tgataaagag ggaaaatgat gatcaaatca aaaaaagtag tggttatttt
    10861 tgttgctttg agttcattct tgctggcagg gtgtgattca ccaaaagatg ctaataagca
    10921 aaactttgaa aaagctatta atactcgact agagaaggaa tgcattacta ttagtccgct
    10981 gggaatgttt gatagtaagc cttatccggt tcaggtagct gtggctcagg ctaatcgata
    11041 tacatctcag gaacgggcag atgaaataaa ttctcgacag ttttctgcgc tggatgtact
    11101 ggtcaaagcc gatttattaa cagtaaaaga taccctggtt gatgacgtta ttggttttac
    11161 taagacgggt aagaaagtgc caggtcgtga atatgctctg acagatgagg ggaaaaaata
    11221 tctgaagtct ccggaacggc ctgatttttg cgttggtcat tataaggtgg atgagatagt
    11281 tgattttacc gaacctggtg atgctatggg aatgaagatc acgcaggtga attacacatt
    11341 cagtcccacc agtatcgctg aatgggcgaa acgcgatgat gttcgggctg catttttagg
    11401 acttgagtct gatttgaaag agaaacaaac aaaacgtatt acactggttt tgaaaaatga
    11461 tggatggtct gctgaacggt aggctgtaat ggtgttgcta cgtgaaaaat accccacgta
    11521 tatcccctac tttttcacgg atttcacgta gcgcaccgcc gttaatgact ctatcaaaaa
    11581 tactcaccga tatttttcgc accgcaaaca acctaactca ctgtaaaaca acaaatcagc
    11641 tttgcgccgg tgacgcatcg atcgcgctgg ccagcgcgat cctcagctgc gaccgctaca
    11701 gtatttttga acaagattac ccttgctccg gtttaattcg ccaggcagtt agacatacag
    11761 gtcattctgt ccagatttca ttagttacaa cattcacatg ttccgttcct ttatccggtg
    11821 ccctgttccg gtctatgtgc ccatatcctg ggttattcag gataaccgct tccttttcgc
    11881 cctgccctgt cgttcatatc cccctggaca aaaatgcatt atcggaaaaa atgtccaaaa
    11941 atttttttgg tgatcggaat ttttgttgtc ggacgcacct ctgcttcagg tcataattgc
    12001 tggcacgtcc aaaaaaacag gtgcggagat taaaaaattg agtcatttac ccgccagcat
    12061 tttccagatt tcacgtagcg acgcctggaa tttaaccctc tctcccgatg cagcccgtaa
    12121 tatgcgggat cttgcagcac agattggcgc aggaatgccg aaatatctga tagcaccgga
    12181 agtctcactg cttctctcct acatcgatga tctgaacatc cgtatgtatt ttgagacgct
    12241 ctggaatacc ggagcacgac tgaacgaagc tctggcactg aaacctgccg attttatgct
    12301 gactccgacg cggcagtacc cgcaacccgt ggtgatcctg aaaacactca agcaacgtct
    12361 aaaagaagca gaaagaagac caggacgccc gaccaccagg catccaccat tacatcctga
    12421 tccgcgatac agaaaaccac aggagcctgc tgtaaggctg gttcctgttc tggatgccgg
    12481 atacgcattg cgtatgcggg aatacctggc gacctggaaa aagcggatca aacatcagcc
    12541 tgtgtgggac ataacatcga gacagacacc gctgaactgg ataaacaccg cagtggcacg
    12601 ggcagagcgt gacggcgtaa cattttcggt tccggtcacg cctcacacgt ttcgccacag
    12661 ttacgccatg cacctgacaa tgagtggcgt tcctccgcgc gtcctgcaat ccctgctggg
    12721 acaccgttat gcaagaagca ccgagattta cacccgcgtt ttttcgctgg acgtcctgac
    12781 aggaagtggc ctgtccttta cctgcgatcc acggcgagca aaacagcttc tcggataccc
    12841 tgaacgcatt aatgatgagt aataatacac accattaaac tgttgccagc acgcaatagt
    12901 acggttatac tttcaccaca aaagattgtg tattaataca cactatttaa ccgggagggt
    12961 tttgtgatgg gaaaaatcat tacagttgca gggcataaag gcggcatagg caaaagcacc
    13021 gtgctttgtt ccttatgtgt ctgtgccatc aggaaaggga aaacagcctg tttcctggaa
    13081 acagactcgc agggaagcat caaggacttc attgaggaac gtaaaaccaa cgagcggctg
    13141 tctgaaatac cgtatttcga gtgctacaca gatattccgg cgatggcacg aaaactggct
    13201 gcccgctttg attatgtgtt tgttgatacg ccaggaatga aatctccggc cttcgttaag
    13261 gcactgagtt gtgctgacat tctgttcaca ttcattgagc caggagcagg catcgagatc
    13321 aacacgctgg gacggctggt tttcgacatt aaaacagcac aggccggagt caatccatca
    13381 atgaaagcat ggattgtcct gaataagtgt tcaactaatc cgtcagacag tgaagcgtca
    13441 gaactgcgcc gacaactcaa tgatgatcct gactggcttc cggttccccg ccagcgtatc
    13501 tatatgcgta ctgcgcataa aaaagcctat aacagtggta tgggcgtaca cgaatacgat
    13561 gataaacggg gaaacaaagc ccgtggagag attgaattac tgttaaaaga aacggatgtt
    13621 ctgtaatgtg tattattaca cactataagc gatgatatac tcacaccctt aatagtgtgt
    13681 ataattacac accttactaa aaacaggata ccgaatctaa tggcaaagct cccaaagaaa
    13741 attgatcagg aaattaaacc gctggattta cgccagtttg taagttctgc tgcacaaaaa
    13801 ccagcatcgg catccgcagt aacgcgtata aatctgacca tgacagatga tgatctggat
    13861 acagcgcagg tgttccagaa caagtacggc gcaagccgtg ctgaagttat cagagcagca
    13921 ctggcagcgt tacgtctggt atcagcagaa gaacgccgga ggctgtttga tgatataagg
    13981 aaaaacagcc cgaaagcagg aagaccaacc gtgaataaat agtgtgtttt attacacacc
    14041 attaaaggag tacaaatatg cttatgaagg ccattaaatc tgtattcaat ttttacatgc
    14101 tctatttgca gccttttatt ggagtggttc ttgcgggtta tggtgtatgg aaattttcag
    14161 gtgacagtat ctcagcatta ctggaaccag catcgtactt tgtcattgga actgttgttt
    14221 tgtttgtatg gtatatagca tggcaaaaag aacgttctaa agaagaagag tttaaatcaa
    14281 gtataaaacc agaagatttt gtgaagtgag gatacggaat aatgccagct tataaccaga
    14341 cattagggca ctttagggga ttttcaatct atggaacact gcatcgttga tttaggcatt
    14401 ataaactatc atcctgtcat tacatggaca gtacctgttg catttttctt tttagtagtg
    14461 ctggctggta tagtattcaa tattggcagg aaaagtaatc taatcgtttt catcctggca
    14521 atgttgctcc cagtaacaac ggttttcatt tttatgttac tgggcggtaa gatcgatatg
    14581 tgctgatcaa ttaaaagagg attatacaat gataaattca atcgcgttta gtcttatagc
    14641 gttatgtata atatatatat catcactact taaaaaaagt aacttcagaa tattttatat
    14701 aaccgtatct acgtgctctt tattattatc atgtgtacca aacaagtacg gtatgattgg
    14761 catagcagca ttggtattta caaccatgtt aggaccatca ttaattattt tagaggataa
    14821 cagacaataa attatgacaa tgcgtttgtt aagcccgcaa atatgtgggc ttatttttaa
    14881 tttctaatat ttttactctt catcatcccg caggaactga caaaaatctt cgctccccat
    14941 catatcgaca gtgtttttaa attcgaaaaa agaaattcct gtcatagcac acacgcgctg
    15001 gataatcagt tttttctttt gctgtctgac ggttgcggtt tcaccaaact ggataacagc
    15061 caggactgtt tcatcatatg atttcaggac aaaatcacag gttatattct gcacttcacg
    15121 gattaaatct tttacagctt cctcgctacc atgaggacga acaaattcaa tgagccgtat
    15181 tttgggaaaa acggaataat caggtataat attacgtgaa agaatgccta aaaactctga
    15241 ttcattattt gagagtaagg gtactttagt aaaacaatca acctccccaa tggctgtaaa
    15301 agcattggtt gtcaaagctg gattgttctt aataaaatca gagaatacat tttcagctga
    15361 tggcaaaaca acagcagaat tatgttgttc atctcgtttt ttattacggt taaatatcat
    15421 ttcatattcc ttttaaaaga gcctgcggca ggcacaggca aaaacatcgt ttttggacaa
    15481 tctgattcaa atgttaacat ttgacatcgg attgtctatc ctgcattgtt ctttactgtc
    15541 tgtaagatcg gcgctcattc ttccggcgtt ctcccgatac caaaaagacg gaacgcaaaa
    15601 cggctttcga tccaccattc aaaaatctgc tctgcgcaac cacaaacagt taaggcgatc
    15661 catgcagcaa gaacaacgta actactgaca tctgcaccca tgtaccaggc gatagttaaa
    15721 cccatcactg cgataccgcc taaaaactgc aacaagaaac gaaatcccgc aagaatcccc
    15781 aatggaataa caacaaccag ataaagggaa gcagccagaa ggtatctgac aggtgtcagc
    15841 agataaaaga acctggatat tttctttttg tcagaataaa cggcatcatt tgcaacatcg
    15901 gttgaatgat cattcatgct gtccctgatt ctttgaaaat ctacgatatt atccttcata
    15961 ttgttatgac ctcaattttc aggtgggtac attttatcaa acatattagt aaactctgag
    16021 tcttccctgg tatcgccctc ataaccaatt gtataagaga actttaattt ataaactttt
    16081 ctcccaattt tttctgaaac tacgaaactt aagttcttta cttccgtatg tttcatgatt
    16141 tcttttactg atttattaag aacatctctt tt
//
LOCUS       DAZFFE010000105        15989 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZFFE010000105 DAZFFE010000000
VERSION     DAZFFE010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.105"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            76..1467
                     /gene="xanP"
                     /locus_tag="ACHJ69_003764"
     CDS             76..1467
                     /gene="xanP"
                     /locus_tag="ACHJ69_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO5016656.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            1588..3297
                     /gene="yicH"
                     /locus_tag="ACHJ69_003765"
     CDS             1588..3297
                     /gene="yicH"
                     /locus_tag="ACHJ69_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO5016657.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWQRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQHWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(3341..4021)
                     /locus_tag="ACHJ69_003766"
     CDS             complement(3341..4021)
                     /locus_tag="ACHJ69_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFO5016658.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDESVKWLL
                     CSEYGILR"
     gene            complement(4002..4934)
                     /locus_tag="ACHJ69_003767"
     CDS             complement(4002..4934)
                     /locus_tag="ACHJ69_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO5016659.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            complement(4982..5842)
                     /locus_tag="ACHJ69_003768"
     CDS             complement(4982..5842)
                     /locus_tag="ACHJ69_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO5016660.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPA
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMNILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSFGKASLY"
     gene            complement(5923..6774)
                     /locus_tag="ACHJ69_003769"
     CDS             complement(5923..6774)
                     /locus_tag="ACHJ69_003769"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO5016661.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVTLALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASAQLCVEGELGHLADAPRYDQAANADLMTQPTDVEP
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEIFHCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGSIGKA"
     gene            complement(6786..7877)
                     /locus_tag="ACHJ69_003770"
     CDS             complement(6786..7877)
                     /locus_tag="ACHJ69_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO5016662.1"
                     /translation="MKENKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            complement(7902..8216)
                     /locus_tag="ACHJ69_003771"
     CDS             complement(7902..8216)
                     /locus_tag="ACHJ69_003771"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO5016663.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADTSAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            complement(8234..8704)
                     /locus_tag="ACHJ69_003772"
     CDS             complement(8234..8704)
                     /locus_tag="ACHJ69_003772"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFO5016664.1"
                     /translation="MDITKILNTNRVILDMQATNKAEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            complement(8731..10284)
                     /locus_tag="ACHJ69_003773"
     CDS             complement(8731..10284)
                     /locus_tag="ACHJ69_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFO5016665.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENIADLLHLDHLLVAECEEIDGVMNNARRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            complement(10579..12897)
                     /gene="yicI"
                     /locus_tag="ACHJ69_003774"
     CDS             complement(10579..12897)
                     /gene="yicI"
                     /locus_tag="ACHJ69_003774"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO5016666.1"
                     /translation="MKISDGNWLIQPDLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDSTLLALGNNDQRPDYAW
                     HEGTAFHLFNLQDGHEAVCEVPTADGSVIFTLKAARTGNTITVTGTGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(12907..14289)
                     /gene="yicJ"
                     /locus_tag="ACHJ69_003775"
     CDS             complement(12907..14289)
                     /gene="yicJ"
                     /locus_tag="ACHJ69_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO5016667.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQCISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     DDLRDIWQNDQWRIVGFLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSVTISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            14582..14676
                     /locus_tag="ACHJ69_003776"
     tRNA            14582..14676
                     /locus_tag="ACHJ69_003776"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14616..14618,aa:Sec,seq:tca)
     gene            14839..>15989
                     /locus_tag="ACHJ69_003777"
     CDS             14839..>15989
                     /locus_tag="ACHJ69_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001414312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5016668.1"
                     /translation="MALTDIQIKRAKPQDKPYTLNDGQGLSLLINPDGSKGWRFRFRF
                     AGKARLMSFGSYDLVSLAEAREKRDTARKQVANGIDPVEERKAQKIAQKLSIETSFEA
                     VSREWHAAKADRWTLAYREEIIKTFEQDVFPFIGKRPIAEIKSLELLEVLKRIEKRGA
                     LGKTRKVRQRCGEVFRYAIITGRAEYNPAPDLAIALAVPKQKHHPFLSAEELPYFVKD
                     LEAYTGSIITKNATKIVMLTGVRTQELRFATWEEVNLEKGIWEIPAERMKMRRPHLVP
                     LSSQVITLFNQLKPISSHYPYIFIGRNNRTKPISKESISQVIELLGYKGRATGHGFRH
                     TMSTILHEQGFDSAWIEMQLAHVDKNSIRGTYNHAQYLEKRLKMLQWYS"
BASE COUNT         4147 a         4164 c         3742 g         3936 t
ORIGIN      
        1 gaggatgtgg caaacgtttg cttttctctt tcggtcagct aaaatgcccg ctttgctacc
       61 acgggattgt tttcgatgtc tgtttccacc ctcgagtcag aaaatgcgca accggttgcg
      121 cagactcaaa acagcgaact gatttaccgt cttgaagatc gtccgccgct tcctcaaacc
      181 ctgtttgccg cctgtcagca tttgctggcg atgttcgttg cggtgatcac gccagcgcta
      241 ttaatctgcc aggcgctggg tttaccggca caagacacgc aacacattat tagtatgtcg
      301 ctgtttgcct ccggtgtggc atcgattatt caaattaagg cctggggtcc ggttggctcc
      361 ggattgttgt ctattcaggg cactagcttc aactttgttg ctccgctgat tatgggcggc
      421 acagcgctga agaccggtgg tgctgatgtt cctaccatga tggcagcttt gttcggcacg
      481 ttgatgctgg caagttgtac cgagatggtg atctcccgcg ttctgcatct ggcgcgccgc
      541 attattacgc cgctggtttc tggcgttgtg gtgatgatta tcggcctgtc gctaattcag
      601 gttgggctga cctccattgg cggcggttac gcagccatga gcgataacac cttcggcgca
      661 ccgaaaaatc tgctgctggc aggcgtggtc ttagccttaa ttatcctgct taaccgtcaa
      721 cgtaaccctt acttacgcgt ggcctcactg gtgattgcga tggcggccgg atatgcgctg
      781 gcgtggttta tgggcatgtt gccagaaagc aacgaaccga tgacacaaga actgattatg
      841 gtgccaacgc cgctctatta cggtcttggc attgaatgga gtctgctgct gccgctgatg
      901 ctggtcttta tgatcacttc gctggaaacc attggcgata tcacggcgac ctctgacgtt
      961 tccgaacagc cggtttccgg tccgctgtac atgaaacgcc tgaaaggcgg cgtgctggca
     1021 aacggcctga actcgtttgt ctcggcggta tttaatacct tcccgaactc ctgcttcggg
     1081 cagaacaacg gggtgatcca gttaaccggt gttgccagcc gctatgtcgg ttttgtcgtc
     1141 gcgctgatgt tgatcgtgct gggtctgttc ccggcagtga gcggttttgt acaacacatt
     1201 ccagaaccgg ttctgggcgg cgcaacgctt gtaatgtttg gcaccatcgc cgcctccggt
     1261 gtgcgtatcg tttctcgtga gccgctgaac cgccgggcga ttctgattat cgcgctgtcg
     1321 ctggcggtcg gtctgggcgt gtctcagcag ccgctgattt tgcagtttgc ccctgaatgg
     1381 ctgaaaaacc tgctctcctc cgggattgcc gcgggcggta ttactgccat cgtgctgaat
     1441 ctgattttcc ccccagaaaa acagtaattc actggcggcg gtaagataat taccgccgca
     1501 gtctcacctc tttcaccatt cccgccttga ggattgcgcg taaatcgtgc ataactccct
     1561 tatgtgcatt tcacgggatg gaagaccatg aaatttattg ggaagctgct tctctacatt
     1621 ctcatcgctc tgttagtggt gatcgctggc ctctattttc ttctgcaaac ccgctgggga
     1681 gcagaacata tcagcgcatg ggtttccgag aatagcgact atcatctggc cttcggggcg
     1741 atggatcacc gtttttccgc gccatctcat atcgtgctgg agaacgtcac gtttggccgt
     1801 gatggccagc ccgcgaccct ggtggcaaaa agtgtcgaca ttgcgctaag cagtcggcaa
     1861 ctgaccgaac cacgccatgt cgataccatc ctgctggaaa acgggacgct gaatctcacc
     1921 gaccagaccg cgccgctacc gttcaaagcc gatcgtctgc aactgcgtga tatggcgttt
     1981 aatagcccga atagcgaatg gaaactgagc gcgcagcggg taaatggcgg cgtggttccg
     2041 tggtcaccag aagccggtaa agtgctgggt acgaaggcgc agattcagtt tagtgccgga
     2101 tcgctttcgc tcaatgatgt tcctgccacc aatgtactga ttgaaggcag tattgataac
     2161 gatcgcgtta cgctgactaa cctgggtgcc gacatcgccc gcgggacatt aaccggaaac
     2221 gcgcagcgta acgctgacgg cagctggcaa gtggaaaacc tgcgcatggc ggatatccgt
     2281 ctacaaagcg aaaaatcgct aaccgatttc tttgcgccat tacgctctgt cccgtcgttg
     2341 cagattggtc gcctggaagt catcgatgct cgtttgcaag gtccggactg ggcggtgacc
     2401 gacctcgatc tcagcttgcg caacatgacc ttcagtaaag atgactggca gacacaggaa
     2461 ggcaaactgt cgatgaacgc cagcgagttt atttatggct cgctgcattt atttgatccg
     2521 attataaacg cagaattttc cccgcagggc gtagcactgc gccagttcac cagccgctgg
     2581 gaagggggta tggtcagaac gtcaggaaac tggcaacgcg acgggaaaac gctgatcctc
     2641 gatgatgcgg caattgccgg actggaatat accttgccaa aaaactggca acagttgtgg
     2701 atggaaacga cacccggttg gttaaacagc ctgcaactga agagatttag cgccagccgc
     2761 aatctgatca ttgatatcga ccctgacttc ccgtggcagc tcaccgcgct cgatggttac
     2821 ggtgccaatc tgacgctggt taccgatcat aaatggggcg tctggagtgg ctcggcgaat
     2881 ctgaatgccg ccgccgcaac gtttaatcgt gttgatgtcc gtcgtccgtc gctggcgcta
     2941 actgccaaca gcagcacggt gaatatcagc gaactgagtg catttacaga aaaaggcatt
     3001 ctggaagcca ccgccagtgt ttcacaaacg ccacaacgtc agacccatat cagcctgaat
     3061 ggacgcggtg tgccggtgaa tattttgcaa cattggggat ggcctgaatt accgttgact
     3121 ggcgacggca atattcagct taccgccagt ggcgatattc aggccaatgt cccgctgaaa
     3181 cctacggtta gcgggcaatt gcatgccgtg aatgccgcaa agcagcaagt gactcaaacc
     3241 atgaatgcgg gcgtagtttc cagtggtgaa gttacgtcga cggagccggt gcagtaaaaa
     3301 taaggacgct agcggatatc agcgctagcg ttccccgtta ttagcgcaaa attccatact
     3361 cactgcacaa taaccatttc accgattcat cttcttctat cgccggaaaa cgtcctcccg
     3421 gctcaagaaa ataattatcg tgttggtcat catttaccat cgagatttcc cagccgagaa
     3481 caaacgcttt ttccgcccag aagcgatgat acagacgcgg cggcaggcaa atgccctgcc
     3541 ccggctttag caccagcgtt tcaccaggtt tcatggtacg ccgctgacca tcaacactca
     3601 cggtaatgac tcgttgatca tcacagagat tggcttcgtt ggcccaggcc aactgcatgc
     3661 ataagtctcc accgccccga ttgaggatat cttccatttt gtgggtgtga aaatgccagg
     3721 gagtctgctg atcttgttgg atctgcaaca ttttctcagc atacggtttt ggatatgccg
     3781 ccgaccccag cgcgccgttg cgcatcgtta gcagtgttag tcccgtttgg ttgaaatcac
     3841 cacgaccaaa atcggtgata tcccaaccga gctgtaagtc gaccacttct tgccagttat
     3901 cccgttcccg ctgcagccag tcatccaccg taaaataggc gaactctggc aaacaaacgc
     3961 ggaaggtctg cgcaatggca tgtgctttat caataacata attaatttgt gaacgtttca
     4021 ttggcttctc cgttgcgcat agcaagccag gcagcgccac gcattccgct actgtcgccg
     4081 tgctgtgctt ttacaatggg agtggtgaat tgatcggtga aaaccagtgg agcgacttct
     4141 gcgttcagat cggtgataag cagttccaca ttcgaaagcc cgccgccgat cacaatcacg
     4201 ccagggtcaa caacattaac gatggttgcc agcgtgcggg caagctgttg gcgaaaacgt
     4261 gccacctgct gcatggcaca agcatcacca cgttgtgcca gggtgacaat cgccgcagga
     4321 gtgagcaagt taccagtcat ctgttgatag cgttcactaa aaccgctgcc ggaaacaaac
     4381 gactccacgc agttgtgttt gccgcaatag caactgactg atggaccatc ttcctgctcc
     4441 gtatagcctg gcaacgtgat atgaccgcat tcggcagcat ttccccaggc ccctataaac
     4501 ggtcgttgct tgatggcaat gccaccgccg cagcccgtac caagcgtaat accaaatacc
     4561 acgtcataat cttgcccggc accgtcgcaa gcttctgata gcgcgaaaca gttaccatca
     4621 ttggctatca ccaccggttg cccaagcaat tgctgcaaat cagcctgtaa cgcatgaccg
     4681 ttaatcacct gaatattcgc gtttttaatc aggccagtga gtggcgagat actgccgggt
     4741 aaggcgatcc ctgtcagcat cggtcgttga acgtcccgcc taatctgctc gataagcgcc
     4801 acaacgcatg agacaaattg ttgatatgtc gacttttgcg tcgggcagcg gtaacggcga
     4861 atctcccagc catgcgcatc catgacaacg gcggcgattt tggtcccacc aatatccaac
     4921 cccagatagt gcatatctcg ttccttgttg cgtaaaaggc gggtcacccc gcccttctgg
     4981 cttagtacag tgatgcttta ccgaacgaac cgaacagttt cattttgtgg cgaatgactt
     5041 cccgcgccgc gttaatcgca tccggataga tgacgttcgg gtcccaccag ctctctttag
     5101 cgagaatttc acgcactttc tggaaatagg cgtatttcat atcgctggag atattgattt
     5161 tgccgacgcc gagcgtaact gattcagcaa tctccgcgtc cgggtttgcc gaaccaccgt
     5221 gtagcaccag cggaatatca atacgtccgg caatgtcgcg caaaatgttc atttgcagct
     5281 tcggctgcat cccttttgga tagatgccat gcgctgtacc aatcgccaca gccagtgtgt
     5341 cggcacctgt acgagcaata aaatcggcgg cctgcgccgg atctgtgtag gtcacttgtg
     5401 aaacaccacc ttctaccgac gttcctgttt gtccgatggt tcccagttca ccctcaaccg
     5461 acacgccaac cgcgtgagcg aggcgaacca cttctgcggt cagcgccacg ttttcttcat
     5521 acggcagcag cgaaccgtcg atcattactg aggtaaatcc gcagcgaatc gcacgcaaaa
     5581 catgctctac agaggcacca tgatcgaggt gcagtacaaa cgggaccgga ctacgctggg
     5641 taatttcgcg aacgtgagcg aaaaaggcat cgcccacaaa atcatgttcg ctgggatgga
     5701 tggcaataat cgccggagta tttgtcgctt ctgcttcatc cacgactgcg cgaaggaaac
     5761 agttatccgc cacattaaat gcgccaatgg caaagccgtg ctggcgggta ggttgcagta
     5821 attctttcat cgaaactaac atgttaaatc tccagtttca gcgtgtagtc cgccctgccg
     5881 gtgcgttatt gcactggcat aaaggccatg acagaaaatg aatcaggctt tgccgataga
     5941 gccgctcgcc tgtaaatacg gtgtaatcgc ttcttcgaag gccagttcca tagctttcag
     6001 cgcgtccacc agttcaatgg atatatcgtg acagagggta tgcaggagcg cggatttccc
     6061 ccgttcaaaa atggccgccc ccacgttgat ttttgctacc cccagcgtcg tacagagctg
     6121 gagctgatcg aacggcgtac cgctaccgcc atgtaatgcc agaggcacgc tcgagcagtg
     6181 gaaaatctcc gccagacgct gaaaatcaat agcaggtaca acaccaggcg cgtacatgcc
     6241 gtgcgccgtg cctaccgaga cggccagtag atcaatgcct gtctgcgcga taaacggttc
     6301 cacatcagtg ggttgcgtca tcaggtctgc attggcggcc tggtcgtaac gcggcgcatc
     6361 tgccagatgg cccagttcgc cttccacgca gagctgcgcg ctggcggcga gttccaccac
     6421 cgccctggtc tggcgcacgt tttcctccag gggaaaagca gaagcatcaa tcattaatcc
     6481 gctgaaacca gcacgaaacg cctgacgaat acagtccgga tttttgccat gatcaagcgc
     6541 cagagtgacc ggtactgagg cagattccgc gagcgtttta accactgccg ccgcgacggg
     6601 tgcaggaaaa tgcgccagat gcccctgata gagattgaga atgatcggag cgcgattctt
     6661 ctccgccacg cggatagctg cgtgagcact ttccagattc atacagttga tcgccagcaa
     6721 ggcgtaatgt tcatgccagg ctttcatcac catacttttc atatcggcaa acatagggcc
     6781 tccggttatt cgagggtgaa agtgatctct tcatctttca gcttttgatc gctcagttcg
     6841 tcaaactctt cgtcgcgttc cgttaccggt ttcttccaga gtgaaagcat cacgccacaa
     6901 atcaccgtgc ctatacccag cgccaggcaa aacagcagag gatgggtgaa cagaggcaca
     6961 acaaacatgc cgccatgcgg aaccggactc tccacgcccc acaccatgat taagccgccc
     7021 gcgaccgccg atgccaccac acaagatccc actactcgca gcaggtcacg cgcggcgatc
     7081 ggaatgactc cttcagtgat catgcagatc cccatcggaa acgcggcttt cagcgcctcg
     7141 cgctccgcac gggtgtattt atggcgtgtc agtaagaacg aaagtgtgat gccaaacgga
     7201 gggatgattg agccgacaaa tttcacggct tctggtccgt agacgccact gaccagcaag
     7261 ccatcggaaa acagcgacat ggttttgttg accggaccac cgaaatcgaa ggttgccatc
     7321 gcgccaagta tggcccccat caggaagcgc gatccgccct gcatcgactc gagcaaatga
     7381 atcagtgctt cttgtaacca ggcgatgggt ttaccaatca gcgtcatcat taacagccca
     7441 ccgataatgg tgcttaatac cggcaacacc atgattggca tgagtccttg catcgactgc
     7501 ggcaggcgga tatagcgttt aagtagcaaa atggtgtagc ccaccaggaa accacccaac
     7561 ataccgccaa taaatccggt atggatttgc ccgcagagaa agccgacaat caggcccggg
     7621 gcaaatccgg gacgatcggc aatagaatag gcaattgccg cgctaatcag cggcacaatc
     7681 agccccattc cccagccgcc aatctgatta agcatccacg ggattgttcc tgttttttca
     7741 ccaacatctg taccgccaag aacttgcccc aacgcaatac atatcccggc ggccacaata
     7801 aggggtatca tccacgagat gccggttaat aaatgttttt taatttcctg tgaaaccgga
     7861 attttatttt ctttcatatt aagatccctc cagtataagg atcatttgtt tcccagtgat
     7921 ttttcgacct tttcaagaaa ttgaacgggc gatttaatga cgatctctgt ctttactcgc
     7981 acaatacgtt tattggtgaa gcgttcttct ccttttattt tcacatctac agcaagaatc
     8041 acgacgtcag cagcagaggt atctgccgcc tgtaattcat tttctgtacc aatggttccc
     8101 tgggtttcaa ctttaatggt atggcccaga gcttttgctc ctttgattaa cttttcacga
     8161 gcaatatagg tatgagcaat acccgccgta caggcggcaa cacagacaat attcatgaca
     8221 tacttcctgt tatttagcag acatcggatt gtgcatactg actaaagaga tcaatgattt
     8281 tttgcggact cgtctcaact aacaattgtt ctatgatgtc atcatccgcc agcgcgcccg
     8341 cgacctggga aagtaaacgg atatgggtgg tattttgatc ttcaagacgt accgcaaata
     8401 aaataataca gcgcacgcta ctgccatcca gcgtttccca gggaatatcc tggcgggtac
     8461 ggccaattgc cagcgtagta ttaattaccg cagaagattt accatgcggg atcgcaatat
     8521 gattttcaaa accagtcgag ccttccgctt cgcgctgcca gacatcttta ataaaggttt
     8581 cccgacaatc gatcgcacca tcttttttga gaagattagt taattcttca atcgcctccg
     8641 ctttattcgt tgcttgcata tctaaaataa cgcgattcgt attcaggatt tttgttatgt
     8701 ccattcgtca tacctcataa aattctgaca ttaaatttca acgacccgag acgtcgcatg
     8761 atgtaatttt tccgtaatca ccatttcagt gacccgctta atatcggcat cattaaaaaa
     8821 agcggtaaca taagcgaccg gcatagtgac tgtaggcaaa ttaatggtcg aaataatcag
     8881 ttcgatattg tcaggcaaaa cctgtgataa attcgctgct gaaataacat caacaatcgt
     8941 ccattcagga aatgctcgca gaatacggct tttcagtaga tgcgaagttc cgattccggt
     9001 tgaacagacc agtaatacac gtttacgggc aatttgccgc tccatcgcag cctgaaaatg
     9061 aacggtcagg taacccactt catcctcgct cactgcattc tcaccccagg ttttaaatac
     9121 ctgattcacc acatattgcg tcaaccgcca gacatccgct aattctgctt taatatcttc
     9181 cagcaatgga ttacggatat gaatgcgata atttagacgg ttaatcagcg gcttaatatg
     9241 caccaataag ccatcatata atgcgctgtc ttcactaaaa tcgcagtcca cgatgtcact
     9301 gaataccgta attaaacgcc aggtaatcag acgcgcttca ttgctggcct gcatatgtga
     9361 aataatgcta acatcttttt gtccatcaat cgccacacct gatgaaatga tatattgata
     9421 aataaaccag acttcatcat cgggcaatgt atgtgcaatt cgttgttcta tcttatgaat
     9481 catctgactg gcaacattaa agatattttc atcatattgc ctggtttgat cttcttctct
     9541 tgataacgca ttcccgtgcg tgtttcgata catcataata aggatgtgag taaaaatgtt
     9601 aacgtaataa ggttctccca aagaccaact taattcattt tccatatcca gtaacaatga
     9661 ctggacaaat aacacctctt cttctccaaa ataatggact aatgctttat agcttccggg
     9721 atccagacgt gaatacacga caccttgcgg ctcattatgg ttaataatgc cgttaatcag
     9781 taatgccatt gcctgtcgga cttgcccttc actaccttca atatgcgtac cacttggact
     9841 gcggatcaac gataacccca acggcgcaag ccaggactca attactctca ggtcattaac
     9901 aatagaggct ccgctgataa agtagcgttc tgacaattta ctgatcgacg tttcattcgg
     9961 tgtttcactt aataactgcg acgctatttt aacgcgcctg gcattattca ttacaccatc
    10021 aatctcttca cattctgcca ccaacaaatg atcaagatgc agcaaatcag caatattctc
    10081 cgcacttaac atcacacccg cgccagcacg cttctcaaca tttattctcc attgtcccag
    10141 ccattgctca agtaaacgta aatcacgttg aatggttttt tccgaaacat ttaaatagcc
    10201 ggcaatcgta acaatagtcg tatattccct tcgaggtaaa aggaatcgta ataatctgtt
    10261 ttgtcgcgag gtaatcattt gcatttttta cccttatcac agattaagaa gccccatatg
    10321 gaagagcttc agttgccatc tctgagggta aatataaagc agaataattt cacaacaata
    10381 ctaaaatctg tccgtaatcc ggacagtgat aaaacaaagc aaggcggtta agggatattt
    10441 ttcagcacag atcacatttt cgtgatgaat aatcgtgatc atgctcgcaa atacgatgga
    10501 agaaatattt attaccagat gtgagattat ttaccggata cgacacaaaa ccattcgtat
    10561 ccggcattct tcaatagatt acaacgtaat tgtcagcgca ttcccttgag gagtcaccac
    10621 cagtccctgc tcactttcag cctgcgaacc gccttgcaga ccatttactt tcacaacatt
    10681 gcgcaagcac agtgtccagt tcttcgcctc gcccgtacca gtcacagtaa tcgtgttgcc
    10741 agtacgtgct gcttttaaag taaagatcac cgaaccgtca gcagtgggca cttcacagac
    10801 agcctcatgc ccgtcttgca gattaaagag gtggaatgcc gtaccttcgt gccacgcgta
    10861 atcgggacgt tgatcgttgt tgcccagcgc cagtagggtg ctatcacgca cataaacggg
    10921 cagactcagg aagctgtgct gctgtttatg ccagcgacta ccgtcgagtt catcgttgtg
    10981 ccacaggtgt gtccagcgac cttccggcag gtagaactgc acatcgcccg cttcagtgaa
    11041 cacaggcgca accatcacgt tgtcgcctaa catatattga cggtcaaggt aatcacaagc
    11101 cggatcgtcc gggaactcca tcatcatggc ccgcatcatc ggcgtacccc gggcgtttgc
    11161 acgtgcagct tcacgataca gatacggcat catgcggcat ttcagttgcg tgaagaagcg
    11221 caccacatca caggactcat catcgtaggc ccacggcaca cgataagatt tgctgccatg
    11281 taaacggcta tggctggaaa gcaaaccaaa cgcgcaccag cgtttgtaaa cgtgcgccgg
    11341 agcggtattt tcaaagccgc cgatatcgtg gctccagaag ccaaaacctg aaaggccaat
    11401 agacaaacca ccgcgcaggc tttccgccat tgattcgtag ttagcgtaac agtcgccacc
    11461 ccagtgtacc ggaaatttct gcgcaccaac ggaggccgag cgggcaaaca agacggcttc
    11521 ttcctcacca acggtgtcct tgagcacgtt ccacaccagt tcgttgtaga tgtacgcata
    11581 atggttatgc attttctgcg gatcggaacc atcaaaccac tgaacatcgg ttggaatacg
    11641 ttcgccaaag tcggtcttaa agcaatcaac gcccatcgcg accagacctt tcagtttgtc
    11701 ggcgtaccat ttgcaggcat ccggattggt aaagtcataa atcgccagac ctggctgcca
    11761 tttatcccac tgccacagcg aaccgtccgg gcgtttgagt aaataacctt tctcttgtaa
    11821 ctctttaaag acgggggatt tttgcccaat ataggggtta atccagacgc agattttcag
    11881 ccctttcgct ttcagacggc ggatcatccc ttccgggtcc ggaaaagtca gcgggtccca
    11941 ctcaaaatcg caccactgga aggctttcat ccagaaacag tcaaagtgga aaacatgcag
    12001 cggcagattg cgttccgcca taccatcgat aaagctgttt accgtcgctt cgtcgtagtt
    12061 ggtggtaaat gaagtggtta gccacagacc gaaggaccac gcgggcggca gcgccggacg
    12121 gccagtaaaa cgggtataac gatcaagtac cgctttcggc gtcgggccgt cgataacaaa
    12181 gtattcgaga tattcactct caacgctgaa ctgtactttg gagactttct ccgatcccac
    12241 ttcaaaagag acgcattgag gatgattgac cagtacccca taaccacggt tagtcatata
    12301 aaacgggata tttttatacg cctgttcagt acttgtgccg ccgtcccggt tccaggtctc
    12361 taccgtctga ccattgcgca ccagggcagt aaagcgctct cccagaccgt aaactgtttc
    12421 gccaacgcca agatccagcc gctcaaacat ataatttcgt tgattattcg tgtcctgcac
    12481 gtagccgtta tttttcacct gactaccggt aatacgttcg ccgttgcgca gaaaatccag
    12541 tgaccagaac tcacctttgc tgacacgcgc acttaagttg ccacttttaa actcagcgta
    12601 acgttctgtg ttttcgatag tgaccttcac gtcctgcaaa atattgagcg ggtaatgagg
    12661 accgttattc agcgccccct gaaaatgctc aatccgtaca ccgacaatac cttcctgtgg
    12721 ggagaaaaag cgcagcgtaa ataaaggagt atcaagctgc caggtacgtt cacgcacatc
    12781 acgaggggca gcatagacca ccatttcatt accctgctgt tcaacctcga acacctgaag
    12841 cgggtgaatc aaattgaggt caggttgaat caaccagttt ccgtcgctaa ttttcattgc
    12901 cgttccttag ttctgcactt cttgagaggt gaattgttgc tggcaacggc gtttgccctg
    12961 cgccagctgt tccataacgg ttttcagatt gtgcgtcgtg agcgagtagt agcgtttagc
    13021 gataatcgcg ctcagcaagt aacagatcgc cggaacaatc gtgaatagcg cgataatgat
    13081 gctaattgtg acgctgttct gcgctttttc tgccgcatca tatccgccat aagccagcat
    13141 ccagccgata agggcaccac cgaaggccaa ccccagtttg agcacaaaca gcgtgccagc
    13201 aaaactgatc ccggtcaggc gtttaccatt acaccactcg ccgtagtcga cggtatcgga
    13261 catcattacc cactggatag gtgtcaccag ttgatgcaac acaccaatca cgaagatgaa
    13321 gacaaacatg gtgatgctgg cctgcatagg aacaaagaac atcgcgaggc taatcactgc
    13381 cagcagggcg ttcgtccacc agaagatagt gactttacat ttccagtcgg tcagcggttt
    13441 tgccagtgcg gagccaatca ggttacccac gcaataagtg gtgagaaaag cgacaaacac
    13501 ttccggcgta cccaaaatcc atgtgacgta atacatcatc gccccaccgc gtacgcacac
    13561 cgccaggata ttgaaaattg tcaggaaacc gacaatacgc cactggtcgt tttgccagat
    13621 atcacgtaaa tcatcccgca tagacgtcgt tgtaggtggt gcttcaacgc gttctttagt
    13681 ggtgaagaaa cagaatgcca gcatcataaa tgccaccacg gaaaggaccg cgataccgcc
    13741 ctggaaaccg agtggtttat tatcaccacc aattaaatta accagtggca tcatcagaac
    13801 agtagagagc atgcctcccg cggtcgccag cacaaaacgc caggattgca gcgagataca
    13861 ctgagtcggg tcattggtga ttacaccacc caatgcgcag taagggatat tgacgatggt
    13921 ataaagtaag gtaagtaggg tgtaagtaat tgctgcataa atcatttttc cgttcatact
    13981 gagatctggc gtgctatagg ccagtacaca gacgatcccg aatggcagtg cgccaaacag
    14041 cacccacggc cgaaatttac cccagcgaga gcgcgttcga tcggccaaca accccatgca
    14101 aggatcggaa atcgcatcca gtgcacgagc gaccaaaaac atggttccga caaatccggc
    14161 aggaatgcca aaaatatcgg tataaaagaa catcatatat aacattacgt tatcgaaaat
    14221 aatgtggctg gcggcgtctc ccatgccata accaattttc tctttaacgg acaacacttc
    14281 actcttcatc tgcttttcct tgatgctata ggggtgctga gaccgataac gcattttgta
    14341 aaccatccct gcgagagcga atattccgat atctggttat taatttatga ttcttgtttt
    14401 atgtgatcgt ggtagcgtta attttgctca tatatcattg taaaatatga gttttatatg
    14461 aactataatg ctttcgtgat aatacgctgc gtgtattagg cggaaaaaac tgatctgggg
    14521 gatgtagaaa ctcaaggaag tagctataat gcgccccgcc tccatgtagc aatcgaggcg
    14581 cggaagatcg tcgtctccgg tgaggcggct ggacttcaaa tccagttggg gccgccagcg
    14641 gtcccgggca ggttcgactc ctgtgatctt ccgccaaact tcatccagcc agttccgaga
    14701 cgctccataa aaccctttgt attcaatacg atgaataatc atcttatccg taatagtccg
    14761 agttcatctc cctgaatccg cgaaaaatat gtatagtaat gtgtatagat tttttatacg
    14821 tagttttcag ttatacacat ggctctaacc gatatacaga tcaaacgtgc aaaaccacaa
    14881 gacaagccat acacattgaa cgatggacaa ggtctgtcgt tgcttatcaa tccagatggc
    14941 tcgaaaggct ggcgtttccg tttcagattt gctgggaaag cgcggttaat gtcatttggc
    15001 agctatgatt tagtaagcct cgcagaagca cgtgagaaac gcgatacggc acgtaaacag
    15061 gttgcaaacg gcattgatcc agtagaagaa cgtaaggctc agaaaattgc tcagaaatta
    15121 tcaattgaaa cctcttttga ggcagttagc agagaatggc acgcagcaaa agcagatcgt
    15181 tggactctag cttatcgcga agaaattatt aaaacatttg aacaggatgt ttttcctttc
    15241 attggtaaac ggccaatagc tgaaattaaa tccctagaac tgcttgaagt acttaaacgc
    15301 atagaaaaac gtggagcgtt ggggaaaacc agaaaagtac gtcaacgatg cggagaggtg
    15361 tttcgctacg cgatcatcac tggccgtgct gaatacaatc cagcacctga tttagctatc
    15421 gcattagctg taccgaagca aaaacatcat ccttttctat cagcagaaga actcccttat
    15481 tttgtcaaag atctggaggc ttacactggc agcataatca ctaaaaatgc aaccaagatt
    15541 gtgatgctta ccggagtaag aacacaagag ctgcgttttg ccacatggga agaagtcaat
    15601 ttagaaaaag gaatatggga aataccagca gaacgcatga aaatgcgtcg gcctcacctt
    15661 gtacctttat catctcaagt gattacccta tttaatcaat taaaacccat ctctagccat
    15721 tatccttata tatttattgg acgtaataat cgtactaaac caatttctaa agaaagtata
    15781 tcacaagtga tcgaattact tggttataag ggacgtgcaa caggccacgg gtttagacat
    15841 acaatgtcga caatactaca tgaacaaggt tttgatagtg catggattga aatgcaacta
    15901 gctcatgttg ataaaaatag cattagggga acatataatc atgctcaata tttagagaaa
    15961 agattaaaga tgttacaatg gtatagtga
//
LOCUS       DAZFFE010000106        15857 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZFFE010000106 DAZFFE010000000
VERSION     DAZFFE010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.106"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..251
                     /locus_tag="ACHJ69_003778"
     CDS             <1..251
                     /locus_tag="ACHJ69_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO5016669.1"
                     /translation="GEPGFAADDSVGVLEYVNDEGVTVREEMKPEMGDYGRVYDALYQ
                     TITNGAPNYVKESEVLTNLEILERGFEQASPSAVTLAR"
     gene            374..1069
                     /gene="yhhW"
                     /locus_tag="ACHJ69_003779"
     CDS             374..1069
                     /gene="yhhW"
                     /locus_tag="ACHJ69_003779"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO5016670.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALVKDEQSVHQIAAERRVWILVVKGNVTINGVKASTSDGLAIWDEQAISIHAN
                     SDSEILLFDLPPV"
     gene            1162..2157
                     /gene="gntR"
                     /locus_tag="ACHJ69_003780"
     CDS             1162..2157
                     /gene="gntR"
                     /locus_tag="ACHJ69_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO5016671.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDIAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            2296..2823
                     /gene="gntK"
                     /locus_tag="ACHJ69_003781"
     CDS             2296..2823
                     /gene="gntK"
                     /locus_tag="ACHJ69_003781"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO5016672.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RCNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            2827..4167
                     /gene="gntU"
                     /locus_tag="ACHJ69_003782"
     CDS             2827..4167
                     /gene="gntU"
                     /locus_tag="ACHJ69_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO5016673.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRVH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(4224..4817)
                     /gene="yhgN"
                     /locus_tag="ACHJ69_003783"
     CDS             complement(4224..4817)
                     /gene="yhgN"
                     /locus_tag="ACHJ69_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO5016674.1"
                     /translation="MNEIISATVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            5009..6112
                     /gene="asd"
                     /locus_tag="ACHJ69_003784"
     CDS             5009..6112
                     /gene="asd"
                     /locus_tag="ACHJ69_003784"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO5016675.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELANPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            6385..8571
                     /gene="glgB"
                     /locus_tag="ACHJ69_003785"
     CDS             6385..8571
                     /gene="glgB"
                     /locus_tag="ACHJ69_003785"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO5016676.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANLFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPSVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNHTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGAVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            8568..10541
                     /gene="glgX"
                     /locus_tag="ACHJ69_003786"
     CDS             8568..10541
                     /gene="glgX"
                     /locus_tag="ACHJ69_003786"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO5016677.1"
                     /translation="MTQLAIGKPTPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGVFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALME
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            10559..11854
                     /gene="glgC"
                     /locus_tag="ACHJ69_003787"
     CDS             10559..11854
                     /gene="glgC"
                     /locus_tag="ACHJ69_003787"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO5016678.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            11854..13287
                     /gene="glgA"
                     /locus_tag="ACHJ69_003788"
     CDS             11854..13287
                     /gene="glgA"
                     /locus_tag="ACHJ69_003788"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO5016679.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            13306..15753
                     /gene="glgP"
                     /locus_tag="ACHJ69_003789"
     CDS             13306..15753
                     /gene="glgP"
                     /locus_tag="ACHJ69_003789"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO5016680.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNDLQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3663 a         3946 c         4381 g         3867 t
ORIGIN      
        1 cgggcgaacc aggattcgca gcggatgatt cggtcggtgt gctggagtat gtcaatgacg
       61 aaggcgtgac ggtaagagaa gagatgaagc cagagatggg cgattatggg cgcgtttatg
      121 atgcgttgta tcaaaccatc accaacggtg cgccaaatta cgtcaaggaa tctgaagttc
      181 ttacgaatct ggaaatcctt gaacgcggtt ttgagcaagc ttctccctcc gcagtgactc
      241 tcgcgaggta agtttgatgg cccctcgaat agttcaattt ttttgaacag aggggtcaat
      301 tttcaccctc tatcatccca ggcggatcgg gtccacacta agcccatcga aatcattcag
      361 ggggcgaata caaatgatct acttacgcaa agcaaatgaa cgcggtcatg caaatcatgg
      421 ctggctggac tcctggcata ctttctcttt tgccaactat tacgatccga actttatggg
      481 cttctctgcg ctgcgcgtga ttaacgacga cgtgattgaa gcagggcagg gcttcggcac
      541 tcacccgcat aaagatatgg aaattttgac ctacgtgctg gaaggtactg ttgagcacca
      601 ggacagcatg ggcaataaag agcaggttcc ggcgggcgag ttccagatta tgagtgcggg
      661 tacgggtatt cgtcactcag agtacaaccc aagcagcacc gagcgtctgc atctgtatca
      721 gatctggatc atgcctgaag aaaacggtat tacgccgcgt tatgaacagc gtcgcttcga
      781 tgccgtgcag ggcaaacaac tggtgctttc gccggatgcg cgagatggtt cattgaaagt
      841 gcatcaggat atggaactgt atcgttgggc gctggtgaaa gatgagcagt cggtgcatca
      901 gattgccgct gaacgccgcg tctggatcct ggtggtgaaa ggtaatgtca ccattaacgg
      961 cgtaaaagct tcgaccagcg atggtctggc aatctgggat gagcaggcga tttccatcca
     1021 tgctaatagc gacagcgaaa tcctgctgtt cgatttaccg ccagtctaaa tttatctcac
     1081 aaaccttcct gaggaatcag gaaggttgtg atgcgtccgt gttaaactaa gagaatctat
     1141 ctcttttgta ccttcaggac gatgaaaaag aaaagacccg tacttcagga tgtggctgac
     1201 cgtgtaggcg tgaccaaaat gacggtcagc cgttttttac gcaacccgga gcaggtttcc
     1261 gtcgctctac gcggcaagat tgccgccgct cttgatgaac tgggttatat tcccaatcgc
     1321 gcgcccgata tactctctaa cgccaccagc cgggcgattg gcgtcctgtt accttctctc
     1381 accaaccagg ttttcgcgga agtattacgc ggaatcgaaa gcgtcaccga cgcgcacggt
     1441 tatcaaacca tgctggcgca ctacggttat aaaccggaaa tggagcaaga acgcctcgaa
     1501 tcaatgctct catggaatat cgacggcctg atcctcaccg aacgtaccca cacgccgcgt
     1561 accttaaaga tgattgaagt ggcggggatt ccggtggtgg agttgatgga cagtaagtcg
     1621 ccgtgcctcg atatcgccgt cggttttgat aactttgaag cggcacgcca gatgaccacc
     1681 gccattattg ctcgcgggca tcgtcacatt gcctatctcg gcgcacgcct cgacgaacgt
     1741 actatcatca aacagaaggg atacgaacag gcgatgctgg atgcaggcct ggtgccgtat
     1801 agcgtgatgg ttgagcaatc ttcttcttac tcttccggta ttgaactgat tcgccaggcg
     1861 cggcgggaat atccgcagct ggatggcgtg ttctgtacta atgatgacct ggcggtcggc
     1921 gcggcgtttg aatgtcagcg tctggggttg aaagttcctg acgatatagc gattgccggt
     1981 ttccatggtc atgacattgg tcaggtgatg gagccacggc tggcgagtgt gctgacgccg
     2041 cgtgagcgga tgggcagcat tggcgctgag cgcctgctgg cgcgtattcg tggcgaatct
     2101 gtgacaccga aaatgttaga tttaggtttc accttgtcac cgggcggatc tatttaagcc
     2161 tacaaatttg aagtagctca cacttataca cttaaggcac ggatggatat tgcttctgat
     2221 attgtccggc tggacaatgt taccgataac agttacccgt aacattttta attcttgtat
     2281 tgtgggggca ccactttgag cacgactaac catgatcacc acatttacgt cttgatgggc
     2341 gtatcgggca gcggcaaatc tgcggtcgcc agtgaagtgg cgcatcaact tcatgccgcg
     2401 tttcttgatg gcgatttcct ccatccgcgc tgcaatatcg aaaaaatggc gtctggcgaa
     2461 ccgctgaatg acgacgatcg caaaccgtgg ttgcaggcgc tgaacgacgc cgcgtttgct
     2521 atgcagcgca ccaataaagt atcgctgatc gtctgttctg cactgaaaaa acactatcgc
     2581 gacttgctgc gtgaaggtaa tcctaatctc tctttcatct acctgaaagg cgattttgat
     2641 gtgattgaaa gccgcctgaa agcgcgcaaa ggccatttct ttaaaaccca aatgctggtg
     2701 acgcagtttg aaacgctgca ggagccgggt gcggacgaaa ccgatgtact ggtggtggat
     2761 attgatcaac cgctggaagg tgttgtggca agcaccattg aggttattaa aaaaggcaaa
     2821 taagtagtga ctacattaac gcttgtttta acagcagtag ggtctgtttt actgctgctg
     2881 tttttagtca tgaaggcgcg tatgcacgct ttcctggctt taatggtggt gtccatgggg
     2941 gctggccttt tttccggtat gccgctcgat aaaatcgcag cgacgatgga aaaagggatg
     3001 ggaggcaccc tcggcttcct ggcggtggtt gtcgccctgg gagccatgtt tggtaagatc
     3061 ttacatgaaa ccggcgcggt cgatcagatt gccgtcaaaa tgctcaaatc cttcggtcac
     3121 agccgcgtgc attatgccat cggccttgcg gggctggttt gtgcgctacc gctgttcttt
     3181 gaagtggcga ttgttctgct gattagcgtt gctttctcaa tggcgcgcca caccggcacg
     3241 aacctggtga agctggtaat cccattattt gcaggcgtgg cggcagcggc tgcgttcctg
     3301 gtgcctgggc cagcgccaat gctgctggca tcgcagatga acgccgactt tggctggatg
     3361 atcctgattg gcctgtgcgc ggcaattccg ggaatgatta ttgccgggcc gctgtggggt
     3421 aatttcatca gccgctacgt ggagttgcat attcctgacg acatcagcga accgcatctc
     3481 ggcgaaggca aaatgccgtc cttcggattc agcctgtcgc tgatcctgtt gccactggtg
     3541 ctggtggggc tgaaaaccat tgccgcgcgt tttgtgccag aaggctctac tgcttacgaa
     3601 tggttcgagt ttattggtca tccgtttacc gcgattctgg ttgcttgcct ggtggcgatt
     3661 tacggtctgg caatgcgtca gggtatgcca aaagacaaag tgatggagat ttgcggtcac
     3721 gcgctgcaac cggcggggat cattctgctg gtgattggtg cgggcggcgt gttcaaacag
     3781 gtgctggttg actctggtgt cggtccggca ctgggcgaag cgttaaccgg catgggcctg
     3841 ccgattgcca tcacctgctt tgtgctggct gcggcggtgc gtatcattca gggttctgcc
     3901 accgttgcct gtttaacggc ggtgggactg gtgatgccgg ttattgaaca actgaactac
     3961 tccggtgcgc aaatggcggc gctgtcgatt tgtatcgccg gtggttcgat tgttgtcagc
     4021 cacgttaacg acgctggttt ctggttgttc ggtaaattta ccggcgcgac cgaagccgaa
     4081 acgctgaaaa cctggaccat gatggaaacc atactcggca ctgttggtgc catcgttggg
     4141 atgattgctt tccagctgtt gagttaagtt tgttcgcccg gtagttgtga cgctaccggg
     4201 ctcttttcga aaaactctcc tcgttacccc ttcatccaca ttcgaatgcc gtcgaggaac
     4261 atctgagttg ccatcatcac cagaatcaat cccatcaggc gttcaagtgc gttcacccct
     4321 ttctcgccca gcagacgtaa aaatagcgaa gactgtagca ggatgacaaa ggtgccgccc
     4381 caggccagca gcagagcaat caccagatgt cccatctgat tcgggtactg atgagacaac
     4441 aacatcagcg tggcgagaat agtcggcccg gcgaccagcg gaattgccaa cggcacgata
     4501 aatggctctt cacctgccgg aagcccgctg ctatttcctg aagcgctggg gaaaatcatc
     4561 ttaatggcga tcagaaacag aatgatgccg ccagaaatgg agacggtttc tgcccgcagg
     4621 ctaagaaatg ccagaatttt ctcgcccgca aacaggaaca ccagcatcac aaggagagca
     4681 ataagcaact ctcgcaccat gattgcccgc cgtcttttcg gttcagtatg tttcagtacg
     4741 gacatgaaaa taggtaggtt tccgagcgga tccataatca ggatcaataa aactgttgca
     4801 gaaatgattt cattcataac tcaaattccc tgataattgc cgcggacttt ctgcgtgcta
     4861 acaaagcagg ataagtcgca ttactgatgg cttcgctatc attgattaat ttcacttgcg
     4921 actttggctg ctttttgtat ggtgaaagat gtgccaggag gagaccggca catttataca
     4981 gcacacatct ttgcaggaaa aaacgcttat gaaaaatgtt ggttttatcg gctggcgcgg
     5041 tatggtcggc tccgttctca tgcaacgcat ggttgaagag cgcgacttcg acgccattcg
     5101 ccctgtcttc ttttctactt ctcagcttgg tcaggctgcg ccgtcttttg gcggaaccac
     5161 tggcacactt caggatgcct ttgatctgga ggcgctaaag gccctcgata tcattgtgac
     5221 ctgtcagggc ggcgattata ccaacgaaat ctatccaaag cttcgtgaaa gcggatggca
     5281 aggttactgg attgacgcag catcatctct gcgcatgaaa gatgacgcca tcatcattct
     5341 tgaccccgtc aatcaggacg tcattaccga cggattaaat aatggcatca ggacttttgt
     5401 tggcggtaac tgtaccgtaa gcctgatgtt gatgtcgctg ggtggtttat tcgccaatga
     5461 tcttgttgat tgggtgtccg ttgcaaccta ccaggccgct tccggcggtg gtgcgcgaca
     5521 tatgcgtgag ttattaaccc aaatgggcca tctgtatggc catgtggcag atgaactcgc
     5581 gaacccgtct tctgctattc tcgatatcga acgcaaagtc acaaccttaa cccgtagcgg
     5641 tgagctgccg gtagataact ttggcgtgcc gctggcgggt agcctgattc cgtggatcga
     5701 caaacagctt gataacggtc agagccgcga agagtggaaa gggcaggcgg aaaccaacaa
     5761 gatcctcaac acatcttccg taattccggt agatggttta tgtgtgcgtg tcggggcatt
     5821 gcgctgccac agccaggcat tcactattaa attgaaaaaa gatgtgtcta ttccgaccgt
     5881 ggaagaactg ctggctgcgc acaatccgtg ggcgaaagtt gttccgaacg atcgggaaat
     5941 cactatgcgt gagctaaccc cagctgccgt taccggcacg ctgaccacgc cggtaggccg
     6001 cctgcgtaag ctgaatatgg gaccggagtt cctgtcagcc tttaccgtgg gcgaccagct
     6061 gctgtggggg gccgcggagc cgctgcgtcg gatgcttcgt caactggcgt aatctttatt
     6121 cattaaatct ggggcgcgat gccgcccctg ctagtgcgta atacaggagt aagcgcagat
     6181 gtttcatgat ttaccgggag ttaaatagag cattggctat tctttaaggg tggcggaata
     6241 cacgagtatt cacagcctta cctgaagtga ggacgacgca gagaggatgc acagagtgct
     6301 gcgccgttca ggtcaaaaaa atgtcacaac cagaagtcaa aaattcagtt ggatggggtg
     6361 acacaataaa acaggaagac aagcatgtcc gatcgtatcg atagagacgt gattaacgcg
     6421 ctaattgcag gccattttgc ggatcctttt tccgtactgg ggatgcataa aaccaccgcg
     6481 ggactggaag tccgtgccct tttacccgac gctaccgatg tgtgggtgat tgaaccgaaa
     6541 accgggcgca aactcgcaaa actggagtgt ctcgactcac ggggattctt tagcggtgtc
     6601 attccgcgac gtaagaattt tttccgctat cagttggctg ttgtctggca tggtcagcaa
     6661 aacctgattg atgatcctta ccgttttggc ccgctaatcc aggaaatgga tgcctggcta
     6721 ttatctgaag gtactcacct acgtccgtat gaaaccttag gcgcgcatgc agatactatg
     6781 gatggcgtca caggtacgcg tttttctgtc tgggctccaa acgcccgtcg ggtctcggtg
     6841 gttgggcaat tcaactactg ggacggtcgc cgtcatccga tgcgactgcg taaagagagc
     6901 ggaatctggg aactgtttat ccccggggcg cataacggtc agctctataa atacgagatg
     6961 attgatgcca atggcaactt gcgcctgaag tccgaccctt atgccttcga agcgcaaatg
     7021 cgcccggaaa ccgcgtctct tatttgcggg ctgccggaaa aggttgtgca gactgaagag
     7081 cgcaaaaaag cgaatctgtt tgatgcgcca atctctattt atgaagttca ccttggctcc
     7141 tggcgtcgcc acaccgacaa caatttctgg ttgagctacc gtgagctggc cgatcaactg
     7201 gtgccttatg ctaaatggat gggctttacc catctcgagc tactgcccat taacgagcat
     7261 cccttcgatg gcagttgggg ttatcagcca accggcctgt atgcaccgac ccgccgtttt
     7321 ggtactcgcg acgacttccg ttatttcatt gatgccgcac acgcagctgg tctgaacgtg
     7381 attctcgact gggtgccagg ccacttcccg accgatgact ttgcgcttgc cgaatttgat
     7441 ggcacgaact tgtatgaaca cagcgatccg cgcgaaggct atcatcagga ctggaacacg
     7501 ctgatctaca actatggtcg ccgtgaagtc agtaacttcc ttgtcggtaa cgcactttac
     7561 tggattgaac gttttggtat tgatgcgctg cgcgtcgatg cggtggcgtc aatgatttat
     7621 cgcgactaca gccgtaaaga gggggagtgg atcccgaacg aatttggcgg tcgcgagaat
     7681 cttgaagcga ttgaattctt gcgtaatacc aaccgtattc ttggtgagca ggtttccggt
     7741 gcagtgacaa tggcggagga gtctaccgat ttccctagcg tttctcgtcc gcaggatatg
     7801 ggcggtctgg gcttctggta caagtggaac ctcggctgga tgcatgacac cctggactac
     7861 atgaagctcg acccgattta tcgtcagtat catcacgata aactgacctt cgggatgctc
     7921 tacaactaca ctgaaaactt cgtcctgccg ttgtcgcatg atgaagtggt ccacggtaaa
     7981 aaatcgattc tcgaccggat gccgggcgac gcatggcaga aattcgctaa cctgcgcgcc
     8041 tactacggct ggatgtgggc attcccgggc aagaaactac tgttcatggg gaacgaattt
     8101 gcccagggcc gcgagtggaa ccatgacgcc agcctcgact ggcatctgtt ggaaggcggc
     8161 gataactggc accacggtgt ccagcgtctg gtgcgcgatc tgaaccacac ctaccgccac
     8221 cataaagcaa tgcatgaact ggattttgac ccgtacggct ttgaatggct ggtggtggat
     8281 gacaaagaac gctcggtgct gatctttgtg cgtcgcgata aagagggtaa cgaaatcatc
     8341 gttgccagta actttacacc ggtgccgcgt catgattatc gcttcggtat taaccagccg
     8401 ggtaaatggc gtgaaatcct caataccgat tccatgcact atcacggcag taatgcaggc
     8461 aatggcggcg cagtacacag cgatgagatt gccagccacg gtcgtcagca ttcactgagc
     8521 ctgacgctac cacctctggc cactatctgg ctggttcggg aggcagaatg acacaactcg
     8581 ccattggcaa acccactccc ctcggcgcgc attacgacgg tcagggcgtc aacttcacac
     8641 ttttctccgc tcatgccgag cgggtagagc tgtgtgtctt tgatgctaat ggccaggaac
     8701 atcgctatga cttgccaggg cacagtggcg acatttggca cggttatctg ccggatgcgc
     8761 gcccgggttt gcgttatggt tatcgcgttc atggcccctg gcaacctgcc gaggggcatc
     8821 gctttaaccc ggcgaagttg ttgattgatc cttgcgcgcg gcaaattgac ggggagttta
     8881 aagataaccc gctgctgcac gccggtcata atgaacctga ctatcgcgac aacgccgcca
     8941 ttgcgccgaa atgcgtagtg gtggttgatc actatgactg ggaagatgat gccccgccgc
     9001 gcacgccgtg gggcagcacc atcatttatg aagcccatgt caaaggatta acgtatctgc
     9061 acccggagat cccggtcgag atccgcggca cttataaagc cctggggcat ccggtgatga
     9121 tcaactattt gaaacagttg ggcattactg cgctggagtt gctgccggtg gcacaatttg
     9181 ccagtgaacc acgtctgcaa cgcatggggc ttagtaacta ctgggggtac aacccggtgg
     9241 cgatgtttgc gctgcatccg gcgtatgcct gctcgccaga aacggcgctg gatgagtttc
     9301 gcgatgcaat caaagcactg cataaagcgg gtatcgaagt cattcttgat atcgtgctca
     9361 accatagtgc ggaactggac ctcgacggcc cgttattctc gctgcgtggg atcgacaacc
     9421 gtagctatta ttggataaga gaagacggcg attatcacaa ctggaccggt tgtggtaaca
     9481 cgctcaattt gagtcatccg gcggtggtag attatgccag cgcctgcctg cgttattggg
     9541 tagaaacctg ccacgtcgat ggtttccgct ttgatctggc ggcagtcatg ggccgtacgc
     9601 cagagtttcg tcaggatgcg ccgttgttta ccgctatcca aaactgcccg gtgctctcgc
     9661 aggtgaagtt aattgctgaa ccgtgggata tcgctcctgg tggttatcag gtgggaaatt
     9721 tcccgccgct gtttgccgag tggaacgatc atttccgcga tgcagcccgt cgtttctggc
     9781 tgcattatga tttacctctg ggagtgtttg ccgggcgttt tgctgcctcc agcgatgttt
     9841 ttaaacgtaa tggtcgtctg ccgagtgccg cgattaatct cgtcaccgcg catgacggtt
     9901 ttacgcttcg cgactgcgtt tgcttcaacc ataaacacaa tgaagcaaac ggagaagaaa
     9961 atcgcgacgg gaccaacaac aattacagta acaatcatgg taaagaaggg ttaggcggta
    10021 ctcttgatct ggttgaacgg cggcgcgaca gcattcacgc cctgttaaca acgttgttgc
    10081 tctcccaggg tacgccgatg ttactggccg gtgacgaaca tggtcacagc cagcatggca
    10141 ataacaatgc ctactgtcag gataaccaat taacctggtt ggactggtcg caggcaagca
    10201 gtggtttaac cgcatttacc gccgcgttaa tccatctgcg caagcgcatt cccgctttga
    10261 tggagaatcg ctggtgggaa gaaggcgacg gcaatgtccg ttggctaaat cgatatgctc
    10321 aacctttaag cacggatgag tggcaaaacg ggccgaaaca gctgcaaatt ctgctctcgg
    10381 atcgcttttt gatcgcaatt aacgccacgc ttgaggtaac agagattgtt ttacctgctg
    10441 gggagtggca cgccattccc ccattcgctg gagaggataa cccagtgatt acggctgtct
    10501 ggcagggacc tgcacacgga ttgtgtgtgt tccagagatg ataaaaaagg agttagtcat
    10561 ggttagttta gagaagaacg atcacttaat gttggcgcgc cagctgccat tgaaatctgt
    10621 tgccctgata ctggcgggag gacgtggtac ccgcctgaag gatttaacca ataagcgagc
    10681 aaaaccggcc gtacacttcg gcggtaagtt ccgcattatc gactttgcgc tgtctaactg
    10741 catcaactcc gggatccgtc gtatgggcgt gatcacccag taccagtccc acactttggt
    10801 gcagcacatc cagcgcggct ggtcattctt caatgaagaa atgaacgagt ttgtcgatct
    10861 gctgccagca cagcagagaa tgaaagggga aaactggtat cgcggcaccg cagatgcggt
    10921 cacccaaaac ctcgacatta tccgccgtta taaagcggaa tacgtggtga tcctggcggg
    10981 cgaccatatc tacaagcaag actactcgcg tatgcttatc gaccacgtcg aaaaaggcgc
    11041 acgttgcacc gttgcttgta tgccagtacc gattgaagaa gcctccgcgt ttggcgttat
    11101 ggcggttgat gagaacgata aaattatcga attcgttgaa aaaccagcta acccgccgtc
    11161 aatgccgaac gatccgagca aatctctggc gagtatgggt atttacgtct ttgacgccga
    11221 ctatctgtat gaactgctgg aagaagacga tcgcgatgag aactccagcc acgactttgg
    11281 caaagatttg attcccaaga tcaccgaagc cggtctggcc tatgcgcacc cgttcccgct
    11341 ctcttgcgta caatccgacc cggatgccga gccgtactgg cgcgatgtgg gtacgctgga
    11401 agcttactgg aaagcgaacc tcgatctggc ctctgtggtg ccggaactgg atatgtacga
    11461 tcgcaattgg ccaattcgca cctacaatga atcattaccg ccagcgaaat tcgtgcagga
    11521 tcgctccggt agccacggga tgacccttaa ctcactggta tccggcggtt gtgtgatctc
    11581 cggttcggtg gtggtgcagt ccgttctgtt ctcgcgcgtt cgcgtgaatt cattctgcaa
    11641 cattgattcc gccgtattgt taccggaagt atgggtaggt cgctcgtgcc gtctgcgccg
    11701 ctgcgtcatc gatcgtgctt gtgttattcc ggaaggcatg gtgattggtg aaaacgcaga
    11761 ggaagatgca cgtcgtttct atcgttcaga agaaggcatc gtgctggtaa cgcgcgaaat
    11821 gctacggaag ttagggcata aacaggagcg ataatgcagg ttttacatgt atgttcagag
    11881 atgttcccgc tgcttaaaac cggcggtctg gctgatgtta ttggggcatt acccgcagca
    11941 caaatcgcag acggcgttga cgctcgcgta ctgttgcctg catttcccga tattcgccgt
    12001 ggcgtgaccg atgcgcaggt agtatcccgt cgtgatacct tcgccggaca tatcacgctg
    12061 ttgttcggtc attacaacgg ggttggcata tacctgattg acgcgccgca tctctatgat
    12121 cgtccgggaa gcccgtatca cgataccaac ttatttgcct ataccgacaa cgtattgcgt
    12181 tttgcgctgc tggggtgggt tggggcagaa atggccagcg ggcttgaccc attctggcgt
    12241 cctgatgtgg tgcatgcgca cgactggcat gcaggccttg cgcctgcgta tctggcggcg
    12301 cgcgggcgtc cggcgaagtc ggtgtttact gtgcacaacc tggcctatca aggcatgttt
    12361 tatgcacatc acatgaatga catccaattg ccatggtcat tctttaatat tcatgggctg
    12421 gaattcaacg gacaaatctc tttcctgaag gccggtctgt actatgccga tcacattacg
    12481 gcagtcagcc caacctacgc tcgcgagatc accgaaccgc agtttgccta cggtatggaa
    12541 ggtctgttgc aacagcgtca ccgtgaaggg cgtctttccg gcgtactgaa cggcgtggac
    12601 gagaaaatct ggagtccaga gacggactta ctgttggcct cgcgttacac ccgcgatacg
    12661 ttggaagata aagcggaaaa taagcgccag ttacaaatcg caatggggct taaggttgac
    12721 gataaagtgc cgctttttgc agtggtgagc cgtctgacca gccagaaagg tctcgacctg
    12781 gtgctggaag ccttacctgg tcttctggag cagggcgggc agctggcgct actcggcgcg
    12841 ggcgatccgg tgctgcagga aggtttcctt gcggcggcag cggaataccc cggccaggtg
    12901 ggcgttcaga ttggctatca cgaagcattt tcgcatcgca ttatgggcgg cgcggacgtc
    12961 attctggtgc ccagccgttt tgaaccgtgc ggcttaacgc aactttatgg attgaagtac
    13021 ggtacgctgc cgttagtgcg gcgcaccggt gggcttgctg atacggtttc tgactgttct
    13081 cttgagaacc ttgcagatgg cgtcgccagt gggtttgtct ttgaagatag taatgcctgg
    13141 tcgctgttac gggctattcg acgtgctttt gtactgtggt cccgtccttc actgtggcgg
    13201 tttgtgcaac gtcaggctat ggcaatggat tttagctggc aggtcgcggc gaagtcgtac
    13261 cgtgagcttt actatcgctt gaaatagttt tcaggaaacg cctatatgaa tgctccgttt
    13321 acatattcat cgcccacgct tagcgtagaa gctcttaagc actctatcgc ttacaagctg
    13381 atgtttacga ttggcaagga tccggtcgtc gccaataaac atgaatggct gaacgcaacg
    13441 ttatttgctg tgcgcgatcg tctcgtggag cgctggttac gctcaaaccg tgcccagttg
    13501 tcgcaagaaa ctcgtcaggt ttactacctg tcgatggagt ttttgattgg ccgtacgctt
    13561 tccaacgcca tgttgtcgct aggaatttac gaagatgtac agggcgcact ggaagcgatg
    13621 ggattaaatc tcgaagagct gattgatgaa gaaaatgacc cgggcctcgg taacggtggc
    13681 ctgggccgtc tggcggcttg cttcctcgat tctctggcga cgttagggtt gccggggcgc
    13741 ggttacggca tccgctatga ctacggtatg ttcaagcaga acatcgttaa cggtagccag
    13801 aaagagtcgc ctgactactg gctggaatac ggtaacccgt gggaattcaa acgccacaac
    13861 acgcgctata aagtccgttt tggcggtcgc attcagcagg aaggtaaaaa aacgcgctgg
    13921 attgaaaccg aagagattct gggagtagct tacgatcaga taatcccagg ttacgacacc
    13981 gacgcgacca acacgttgcg tttgtggagt gcccaagcca gtagcgaaat taacctcggt
    14041 aaattcaacc agggcgacta cttcgcggca gtggaagata aaaaccactc cgagaacgta
    14101 tctcgcgtac tgtatccgga cgactccacc tactccgggc gtgagctacg actgcgtcag
    14161 gaatacttcc tggtttcctc gaccattcag gacattttaa gccgccatta tcagttgcat
    14221 aaaacctacg ataacctggc ggataaaatt gcgattcacc tcaacgatac tcatccggtg
    14281 ctgtcgattc ctgagatgat gcgtctgctg atcgatgagc accaatttag ctgggacgac
    14341 gcgtttgaag tgtgttgtca ggtcttctcc tacaccaacc acacgctaat gagcgaggcg
    14401 ctggaaacct ggccagtcga tatgctgggt aaaattctgc cgcgtcacct gcagatcatc
    14461 tttgaaatca acgactattt cctgaaaacc ttgcaggaac agtatccgaa cgataccgat
    14521 ctgctgggac gggcgtcgat cattgatgaa tccaacggtc gtcgtgtgcg tatggcctgg
    14581 ctggcggttg tggtgagcca caaagttaac ggtgtatcgg agctgcactc taacctgatg
    14641 gtgcaatcgc tatttgccga ctttgcgaaa atcttcccgg gtcgtttcac caacgtcacc
    14701 aacggtgtga cgccgcgtcg ctggctggcg gtagcgaacc catcgctttc agccgtgttg
    14761 gacgaacacc tgggccgcaa ctggcgcacc gaccttagcc tgcttaatga tctgcaacaa
    14821 cactgtgatt tcccaatggt taatcacgcg gtgcatcagg cgaagctgga gaacaaaaag
    14881 cgtctggcgg agtatatcgc ccagcagctg aatgtggtgg taaatccgaa agcgctgttc
    14941 gatgtgcaaa tcaaacgtat tcacgaatac aaacgtcaat tgatgaatgt gttgcacgtg
    15001 atcacccgct ataaccgcat caaggccgac ccggatgcga agtgggtacc gcgcgtgaat
    15061 atttttggcg gtaaggcggc ttcggcctat tacatggcga agcacattat tcatttgatc
    15121 aatgacgtag cgaaagtgat caacaacgat ccgcagattg gcgacaagct gaaagtcgtg
    15181 ttcatcccga actacagtgt tagcctggcg cagttgatca ttccggcggc cgatctgtct
    15241 gaacagattt cgctggcggg gacggaagct tccggcacca gtaacatgaa gtttgcgctt
    15301 aacggcgcgc tgactatcgg tacgctggac ggtgcgaatg tcgagatgct ggatcatgtc
    15361 ggtgctgaca atatctttat ctttggtaac acagcggaag aagtggaaga actgcgtcgt
    15421 cagggctaca aaccgcgtga atactacgag aaagatgagg agctgcatca ggtgctgacg
    15481 caaatcggca gcggtgtatt cagtccggaa gatccgggtc gctatcgcga tctggtcgat
    15541 tcgctgatca acttcggcga tcactaccag gtactggcgg attatcgcag ctatgtcgat
    15601 tgtcaggata aagtcgacga actctacgag cgtcaggaag agtggaccgc aaaagcgatg
    15661 ctgaacattg ccaatatggg ctacttctct tctgaccgta cgatcaaaga gtacgccgat
    15721 catatctggc atattgatcc ggtgagattg taagttcaaa tacggctaaa taccgtgtgt
    15781 tccgctgaag taatacagcg tggaactcac ggtatttcta tacacaataa acaataactt
    15841 tgatgtatgt tattcta
//
LOCUS       DAZFFE010000107        15723 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFFE010000107 DAZFFE010000000
VERSION     DAZFFE010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.107"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..180)
                     /locus_tag="ACHJ69_003790"
     CDS             complement(<1..180)
                     /locus_tag="ACHJ69_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5016681.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQ"
     gene            complement(333..899)
                     /gene="slp"
                     /locus_tag="ACHJ69_003791"
     CDS             complement(333..899)
                     /gene="slp"
                     /locus_tag="ACHJ69_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO5016682.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(1089..1468)
                     /locus_tag="ACHJ69_003792"
                     /pseudo
     CDS             complement(1089..1468)
                     /locus_tag="ACHJ69_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2084..2509)
                     /gene="arsC"
                     /locus_tag="ACHJ69_003793"
     CDS             complement(2084..2509)
                     /gene="arsC"
                     /locus_tag="ACHJ69_003793"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO5016683.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGITVRALLRKNVEPYEELGLAEDKFTHDQLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDTQKGAFTKEDGEKVVDEAGNRLK"
     gene            3043..3126
                     /gene="dinQ"
                     /locus_tag="ACHJ69_003794"
     CDS             3043..3126
                     /gene="dinQ"
                     /locus_tag="ACHJ69_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO5016684.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3180..4532)
                     /gene="gorA"
                     /locus_tag="ACHJ69_003795"
     CDS             complement(3180..4532)
                     /gene="gorA"
                     /locus_tag="ACHJ69_003795"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO5016685.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTIEVNGETITANHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4604..5446)
                     /gene="rlmJ"
                     /locus_tag="ACHJ69_003796"
     CDS             complement(4604..5446)
                     /gene="rlmJ"
                     /locus_tag="ACHJ69_003796"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO5016686.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5649..7691
                     /gene="prlC"
                     /locus_tag="ACHJ69_003797"
     CDS             5649..7691
                     /gene="prlC"
                     /locus_tag="ACHJ69_003797"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO5016687.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERIVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERNDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMELFKRFRGREPQLDAMLEHYGIKG"
     gene            7699..8451
                     /gene="rsmJ"
                     /locus_tag="ACHJ69_003798"
     CDS             7699..8451
                     /gene="rsmJ"
                     /locus_tag="ACHJ69_003798"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO5016688.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALSD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8500..9969)
                     /gene="dtpB"
                     /locus_tag="ACHJ69_003799"
     CDS             complement(8500..9969)
                     /gene="dtpB"
                     /locus_tag="ACHJ69_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO5016689.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            10135..10196
                     /locus_tag="ACHJ69_003800"
                     /pseudo
     CDS             10135..10196
                     /locus_tag="ACHJ69_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10286..10720)
                     /gene="uspA"
                     /locus_tag="ACHJ69_003801"
     CDS             complement(10286..10720)
                     /gene="uspA"
                     /locus_tag="ACHJ69_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO5016690.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11111..11446
                     /gene="uspB"
                     /locus_tag="ACHJ69_003802"
     CDS             11111..11446
                     /gene="uspB"
                     /locus_tag="ACHJ69_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO5016691.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11517..13016)
                     /gene="pitA"
                     /locus_tag="ACHJ69_003803"
     CDS             complement(11517..13016)
                     /gene="pitA"
                     /locus_tag="ACHJ69_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO5016692.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     VGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            13248..14441
                     /gene="yhiN"
                     /locus_tag="ACHJ69_003804"
     CDS             13248..14441
                     /gene="yhiN"
                     /locus_tag="ACHJ69_003804"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO5016693.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVAFRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQTLISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLVAE"
     gene            complement(14528..15580)
                     /gene="yhiM"
                     /locus_tag="ACHJ69_003805"
     CDS             complement(14528..15580)
                     /gene="yhiM"
                     /locus_tag="ACHJ69_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HFO5016694.1"
                     /translation="MNIYIGWLFKLIPLLMGIICIALGEFVLTGSGQSEYFVAGHVLI
                     SLSAICLALFTTAFIIISQLTHGMNKFYNRLFPVIGYAGSATTMIWGWSLLASNNVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNASGSKSDGTPLQAYSSTIGNCLI
                     AVPVLLTLFGFIWSVILLRSADITPHYVAGHVLMGLTAICACLIGLVATIVHQTRNTF
                     SVKEHWLWCYWVILLGSLTIIFGIYVLISSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLAAFLAEIAQTDMAYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4094 a         3823 c         3982 g         3824 t
ORIGIN      
        1 ttgagtgaga aaattagaat gaaatacatt attcattaat gtatcaataa tgacgaaggc
       61 attctgatgc aacattacgt cgatctcttc ttcgttctgt atatgaacga cattaccttt
      121 actcagaatg tttttcatcg cggtgaaaaa catcgtatcc ctggtaatta taagaaacat
      181 aaccgactcc tggtacggac attaacttta taatcagttt gccaaaggta attaacagga
      241 cagggcttat aataatagtt agcttactgt gtattaattt gatgtaatgc atgatgcaga
      301 tgctgagaaa gcatctgcat ctttcggtgg tgttatttga ccagctcagg tgttacctga
      361 ctcaccgcat tggtgtagta aggagccccc cagcccggtt ccggccagaa tgcaccatag
      421 ccgtaatccc acaagttata ggtggtatta accacttctc tcaaatgcca cacctggatg
      481 ccctgcatat tcacttccag gaagttatac gggactttat tgataaagcc aggttgttca
      541 ccctgaatgg taccgaggat ggtaacaaag tgattacgat agttcactgg atcaaggaag
      601 ccgctttgtc tggcgagcag tcggccctga tagttggctt caatatcagg cttcgcatag
      661 ctatccaacg gtaatacagc gatttctaac aacgtatccg ttttgccatt gataacgttg
      721 ataaccttcc caccaaagcg cgcttgttga ccaacatata accccggctg gttatgaaca
      781 gcaacaaaac ttttttgaat atcaggttgg ttattgcctt tgatattttg cggaattgaa
      841 ctacatgcgg caagcaaaaa tgaaaggctg aggatgagtg caccttttgt catgttcatg
      901 ttactatcct tatcaacaaa tattaaaacc ataatagata tttctttata gttttcatct
      961 gattctgagg tgatatgcct gatagtaacg taaacctgtc tgatgtttat atctcttctg
     1021 tttagttgag ctcagttaat tttggggcgt ggcatgagta tccattgttc cataatctat
     1081 attcagaggt ttttaatttt ttcgtgtcta atgttattta tctgattaag tattgattgt
     1141 gtcttttgcg gaagtggtgt cgagcttatc gtttcaatgt tattaataag acttacaagt
     1201 tgagcctcaa ctttgagtag tctgcatttg tatatgtggt gttcgtatgg cacgcattaa
     1261 tttgttggcg ttgttgttgc gctaaatcga attgttcaga ggtgtatcct tgtccagaga
     1321 atggggaatt aatgggttgt tgttcttccc ggttatcaca aggccacttc ggtgtgtctg
     1381 gcgttttaac attatcggta tttgttgtgc agttataaat atcaccgtta tatgtattta
     1441 ttacacttgg ggtaaagtta attgacataa cattccttat tcatcatttg ttttatgcta
     1501 agatttttaa attggataat tagaacagga atggattttc ttctacatac agtaaatata
     1561 atgcatcata tcttcctgat ataattacct ttgacttccc ttttatttcc atctggtaat
     1621 atttgagtgc aaaaaaggcg ataataatat cgcaaaagaa aacgttaaca actatcgaaa
     1681 atgctcgttt taaaaagtta tgatgttctc tgatagtgtg tgaagtgata tttttgtgat
     1741 gttttagata tgtaataata aaataggtta acgtatgttg aaatcattta ttgtgtaaac
     1801 cactgataga tcataatcca gtacatcttt aaacagctgc actgtatcgc tgataactaa
     1861 gtagctcact gatacaccat gctggacgcg ataattggaa tctcacgcag ggagtactgt
     1921 gtaaatagca aaatagcctg atgtgctacg actgtcaggc ctacaaataa catacaattc
     1981 atcgaattat cataactttg taggtcggat aaaacgcgcc agcgtcgcat ccgacataaa
     2041 taaagcgcat ttttctaaca acctgtgggg gaatatagcc gctttatttc aggcgattac
     2101 ccgcctcatc aaccactttc tcgccatctt ccttggtgaa cgcgcctttt tgcgtatctg
     2161 gcagaatttc cagcacgact tctgaagggc ggcacaggcg agttcccagc ggcgtcacca
     2221 caatcgggcg attaatcaga atcgggtgct gaagcataaa gtcgattaac tgatcgtgag
     2281 taaatttatc ttctgcaagg cccagttcct catacggctc gacgttttta cgcagcaacg
     2341 cgcgtacggt aatccccata tcggcaatga gttttaccag ctcatcgcgc gtcggtggtg
     2401 tttccagata atagataatg gtcggttctg taccgctgtt gcggatcatc tccagcgtat
     2461 tacgcgacgt accgcaggcc gggttgtgat aaatggtaat gttgctcata tcagtatctc
     2521 attacgatgt gaaagagagg tgtagcacca gcgccaccag agttacaaac caaagcggac
     2581 aagtcaaaaa cacatatggt ttaacgaata tgtaagtgta gagaaaggtg taggtgtgaa
     2641 ttaagcgttg ggcattcatt tgaaaggagg tctgaatcag gacgtaaaaa aaccgacttc
     2701 gcgtcggttt ttttactgtt cagccctgag ttggtggctc tggctggagt gagatagcca
     2761 tcatctaacg catcaatgct aataccatac tgaaactatt gcaaggacat gctggtttta
     2821 taacccgcaa gtactgtatg attatccagt tagttctgag acattttcac tctggcaatg
     2881 cgcataaacg ctttcaaagt cctggtctga agtacgggtg gtgccgttaa ctgatgctct
     2941 ggccggagtg agatagtcgt catctaacaa tgagacatgc gccgtgacag gcaatggatg
     3001 agtaagcgga tgcattctca ctccatcgca tggagaaaac gggtgattga taaagcaatc
     3061 atcgttcttg gggcgttaat tgcgctgctg gaactgatcc gctttctgct tcagcttctg
     3121 aactgatagc ggaaacgtag ctaagggcta agagcacact actcttagcc ctttaacatt
     3181 taacgcattg tcacgaactc ttctgccgct gtcgggtgaa tggcaaccgt gttgtcgaag
     3241 tcttttttgg ttgcacccat cttcagcgcc accgcgaagc cctgcaacat ttcgtccata
     3301 ccaaaaccaa tgccgtgaat accgacaatc ttctcttccg gtccaacgca taccagtttc
     3361 atgcggcacg gctggcggtg agtggtgacg gcggtataca tcgcggtgaa agaggattta
     3421 tacactttca cctgatcgtc gccgtactgc tcgcgcgcct gcggctccgt taaaccaaca
     3481 gtaccaatcg gcggatggct gaagaccacg gtcggaatgt tgctgtaatc cagatgctca
     3541 tccggcttgt tattaaacag gcgttcagag agacggcgac ctgctgcaac agccaccggt
     3601 gtcagttcca ctgcacccgt gttatcgccc accgcgtaaa taccttcaac gttggtgttt
     3661 tgatatttat cgacgacgat ataacctttt tcgttagttt taacgccagc ggcttccagg
     3721 ttgatgttgt cattggcagg ctcgcgacca atcgcccaaa tcaggcaatc caccgtttca
     3781 ctgcgaccat cttccagctc cagcgtcaga ctaccatcgg catttttcac taccgctttc
     3841 gggatggcgt tggtgtgcaa ctgcgggcct tcggcgttca tcacttcaac cagcgtttca
     3901 gagatcatcg ggtcgaagct gcgcagcggc gcatgtttac gcacaaacag atgcgtcttc
     3961 gcaccgaggc cgttaatcac gcccgccagt tcaacggcga tgtaacccgc gccaacaacc
     4021 gctacacgct ctggcaaagc cggaagggcg aagaagccat cagaatcaat accgtattcc
     4081 acgcctggaa tatccgggtg gctcggacga ccaccggtgg cgatcagaat atgattggcc
     4141 gtgatggttt cgccgttgac ttcaatggtt ttggcatcaa cgaagcgggc aaagcctttg
     4201 attacatcaa cgttattttt accgagcacg ttctcatagg aagtatgaat acggtcgata
     4261 taggcggtac ggctggcgat caacgtttcc cagttgaatt tattgatagt ggtatcaaaa
     4321 ccataatccg ggccgtacat atggatcgct tcacggattt gcgccgcgtg ccacatcact
     4381 tttttcggca cacagccaac atttacgcag gtgccgccca gctctttggc ttcaatcagt
     4441 gcgcatttct ggccgtacat agccgcgcgg ttgatggagg cgataccgcc gctgccgccg
     4501 ccgatggcga tgtaatcata gtgtttagtc atgacatatt gtccttatcg ttgattaccg
     4561 cgattgtagc gcgagacgta ataggtgcca gcaatggctg caattactct ggcacgatcc
     4621 agcttacggt ggcgtgcccg gtgcctgccg gaaccagttt gctgtgcagc cacggcagca
     4681 cgttattcat ctgctgttcc agcttccacg gcgggttaat cacaatcatg ccagaagcgg
     4741 tcatgccacg gcgatcgctg tctggcagca ccgccagttc aatttgcaga attttgcgaa
     4801 tgccggtcgc ttccaggtcg tggatcatgc gcttaatttg ctggcgcagc accactggat
     4861 accacaatgc gtaagtgcca gtggcgaaac gtttgtaacc ttctgctatc ccgctgacca
     4921 ccgcctgata gtcggttttc atttcatacg gcgggtcgat aaggattaaa ccacggcggg
     4981 aaaccggcgg cagcttggcc ttaagctgct ggaaaccgtc ggctttttcg acgcgcgcac
     5041 ggctgtcttt ctgaaattca gaacgcagca acgggtaatc gctcgggtgc agttcggtca
     5101 gttgcaggct gtcctgttca cgcagtagct ggcgagcaat caacggcgaa ccggggtaat
     5161 aacgcaactg cccgctacgg ttgaagtgtt ttaccacatt gatgtacgcc tccagctccg
     5221 caggcaaatc gtcctgctgc cagatacggg cgatgccttc gagatattcg ccggtacgct
     5281 cggcatgttc gctgcctaac tgataacgcc cggcccctgc gtgggtgtcg agatagagaa
     5341 acggtttatc tttctctttc agcgactcaa tgatcaggct ctgaacggta tgtttaagga
     5401 cgtcggcgtg gttgccagcg tgaaagctgt ggcgataact gagcatgggt aaaggtgttc
     5461 cggtaagtaa aaatcggccc gtattcgggg cgcacaaaag cctatcagga cagtataccg
     5521 aaaagaggcc gccgccgcga aagcgtaacg tttctcattg aaattcacta cacttaaccc
     5581 catgctacaa acattatgta aagcgcctgt tgagcgcttc cttaacctct ttaaccagga
     5641 ctgcgctaat gacgaatccg ttactgactc cctttgaatt gcctccgttt tctaaaattc
     5701 tcccggaaca tgtcgttcca gccgtgacta aggcgctgaa cgactgccgc gaaaatgtgg
     5761 agcgcatagt agcgcaaggg gcaccgtaca cctgggaaaa tctctgccag ccgttggcgg
     5821 aagtggacga tgtgctgggg cgtatcttct ccccggtcag ccatctgaac tcggtgaaaa
     5881 atagcccgga actgcgtgaa gcgtacgaac aaaccctgcc gctgctgtca gaatacagca
     5941 cctgggtagg gcaacatgaa gggctgtata aggcatatcg cgacctgcgt gatggcgatc
     6001 attacgccac gctgaacacg gcgcagaaaa aagcggttga taacgcactg cgcgacttcg
     6061 aactctctgg catcggtctg ccgaaagaga aacagcagcg ttacggcgaa attgctaccc
     6121 gtctttctga actgggcaat cagtacagca acaacgtcct cgatgcgacg atgggctgga
     6181 ccaaactcgt taccgacgaa gcggagctgg cggggatgcc agaaagcgcg ctggctgcgg
     6241 caaaagccca ggccgaagcg aaagagctgg aaggttattt gctgacgctg gatatcccaa
     6301 gctacctgcc ggtaatgacc tactgcgaca accaggccct gcgtgaagag atgtatcgcg
     6361 cttatagcac tcgtgcttcc gatcaaggtc cgaacgccgg taagtgggac aacagcaagg
     6421 tgatggaaga gatcctcgcg ctgcgtcacg aactggcgca actgctgggc tttgaaaact
     6481 acgccttcaa atcacttgcc actaaaatgg cagaaaaccc gcagcaggtg ctggatttct
     6541 taaccgatct ggcaaaacgc gcgcgcccgc aaggcgaaaa agagctggcg caactgcgcg
     6601 ccttcgccaa agccgaattt ggcgtcgatg agttacaacc gtgggatatc gcttactaca
     6661 gcgaaaaaca gaaacagcac ctctacagca tcagcgatga acaactgcgt ccgtacttcc
     6721 cggaaaacaa agcggttaac ggcctgtttg aagtggtgaa acgtatttac ggcatcaccg
     6781 ctaaagagcg taacgatgtt gatgtctggc atccggatgt acgtttcttc gaactgtatg
     6841 acgagaacaa cgaactgcgc ggcagcttct acctcgacct gtatgcccgt gaaaacaaac
     6901 gcggcggggc gtggatggat gactgcgtag gccagatgcg taaagccgac ggttcgctgc
     6961 aaaaaccggt cgcgtatctg acctgcaact tcaaccgccc ggtaaatggt aaaccagcgc
     7021 tgtttaccca tgacgaagtg atcaccctgt tccacgagtt cggtcacggc ctgcaccata
     7081 tgctgacccg cattgaaacc gctggtgttt ccgggatcag tggggtgccg tgggatgcgg
     7141 tcgaactgcc gagccagttt atggaaaact ggtgctggga gccggaggcg ctggcgttta
     7201 tctccggtca ctatgaaacc ggcgaaccac tgccgaaaga gttgctggat aaaatgctgg
     7261 cggcgaagaa ctaccaggcg gcgctgttta ttctgcgcca gctggagttc ggcctgttcg
     7321 atttccgcct ccatgccgag ttccgtccgg atcagggggc gaaaatcctc gaaactctgg
     7381 cagaaatcaa gaaactggtt gccgtggtac cgtctccgtc atggggccgt ttcccgcatg
     7441 ctttcagcca catttttgcc ggtggttatg cggcaggtta ctacagctac ctgtgggccg
     7501 atgtgctggc ggcagatgcc ttctcgcgtt ttgaggaaga gggcattttc aaccgtgaaa
     7561 ctgggcagtc gttcctcgac aacattctga gccgtggcgg ttcagaagag ccgatggagc
     7621 tgttcaaacg cttccgtggt cgtgaaccac agctggatgc gatgctggag cattacggca
     7681 ttaagggctg aacattcagt gaaaatctgc ttaattgatg aaacaggcgc cggagacggt
     7741 gccttatctg ttctggcggc tcgctggggg ctggaacacg atgaagacaa cctgatggcg
     7801 ctggtgttaa cgccggagca cctggaactg cgcaagcgtg atgagccgaa acttggcggc
     7861 atctttgttg attttgttgg cggagcgatg gcgcaccgac gcaaattcgg cggcggacgt
     7921 ggtgaagcgg tggcaaaagc agtgggcatt aaaggcgatt atttgccgga tgtggtggac
     7981 gccaccgcag gattagggcg cgatgccttt gtgctggctt cggtcggctg ccgcgtgcgg
     8041 atgctggagc gtaatccagt ggttgccgcg ttgctcgacg atggtctggc gcgtggttat
     8101 gcggatgcgg aaatcggcgg ctggttacag gaacgtttgc agttgattca cgcctccagt
     8161 ctgacggcgc tgagcgatat caccccgcgt ccgcaggtag tttatcttga cccaatgttc
     8221 ccgcataagc agaaaagcgc gctggtgaaa aaagagatgc gcgtgtttca gtcgctggtg
     8281 gggccggatc ttgatgccga tggattgttg gagcctgcgc ggttgctggc aaccaaacgc
     8341 gtggtggtga agcgtccgga ctatgcgccg ccgctggcga atgtcgccac gccaaacgcg
     8401 gtagtcacta aagggcatcg ctttgatatt tatgccggaa cgccggtgta aaagtaaagc
     8461 ggggtagcga taacgctacc ccgctttctg caagaataat taatggcttt ccggcgtcgc
     8521 aatcatgcgt ttcagccacg gcaccatcaa tagcatcact actgcaacgc ccagcgtaac
     8581 caggccaatc ttaccaaaca cgttggtata gacgggcaac gtctcaagtg gatcggtaat
     8641 gttgtctggt actgcggtaa atgttgccac atagccgccc agcaagaacg cggcagcctg
     8701 cgtcaggaac cacatcccga gaataaagcc catcaaatgc tgcggcacca gggcagcaat
     8761 catcgccagg ccaagggcgc taataaacag ttcacctaag ctctggaata agtacaccag
     8821 cacgataaac catggcgatg tcagcccctg ggcatccgca aaccacattc cagccgctgc
     8881 agccgtcaaa aagcccagtg agcacataaa catgccgaga gtaaacttca tcggcatcga
     8941 gagatctttg cctttgttac ccagatgcgt gtaaatgcct gccagtattg ggctggcgag
     9001 taccacccag aacgggttaa gcgcctggaa gctgaccggg ttgatggaaa agccgagaat
     9061 ttcatgatgc acgttgttga tggcaaagaa gttcagcgac gttggcatct gggcgtagag
     9121 aatgtaaaac accaccgctt cgagcatcag gacaaaggcg acaaacattt tattgcgccc
     9181 ggttttatcc agcttgaatg cctgacgaaa gaagatgatg gtgacgacga tggagagaac
     9241 aatcagaacc agattggcga cttctacgtt gtgcatcagc caggcgcaga cgaagatcat
     9301 caccacgctg ccaagtaata cgtacaacag tttactgaag ctcatcggcc ggaagtcggg
     9361 ttcagaacca atgtctttca ccattccacg acaggcgatg taaaccagta atgcgataat
     9421 taaccctgca ccgcacaggt tgtaggtgac tgaatagccg aatttatcag cgatcacagg
     9481 ggccagcgat aacgctatca acgagccgat attgatcgac atatagaaca gggtgaatgc
     9541 gccatcaagc cgcggatctt tcggcgggta gcacttcgaa agcaagctgg ctgggttagc
     9601 tttaaacagg ccgttaccga cagcgatagt ccccagggcg atgaaaatca ggtcaggctt
     9661 aagtagcgac atgccggtca tgaagtagcc aatcgccagc acaagtgctc cgagaacaat
     9721 ggtgcgtttg gttcccagca ggtggtcgcc aacatagcca ccaatggaaa tgaggccata
     9781 gaccagcgca gcaaaagcac caaaagtgac aaaagcctgc tcttgcgaga acccaagctg
     9841 tttaacgaag aaaaccgcca gtacgccctg cacgccgtag tagccgaatc gctcccataa
     9901 ctcgacaaaa aagatcatga aaaatgggcg aggttgctgc agcatcccca tgggtgttgt
     9961 tgtattcata tttattaacc ttccaatacc atcctgaaaa gtattaacgc gtaagggata
    10021 acgcgcgaaa aagtttacat ctgtcagaga ttcttaatat tgatgcggag aatagattaa
    10081 tacactatta caacagaaaa taaccagctg atcacactaa tgatagaaaa tatgatggtg
    10141 ttaaatgttg tattggcagg ggttttgatt gcgtacgtag accgcaggcc agataaggcg
    10201 tttacgctgc atcaggtaac aaggcgcgcc aaaaagcaaa acgcccgcat atgcgggcgt
    10261 tcaggaacac gtcgtggagg gaagattatt cttcttcgtc gcgcagcgga acaatcagca
    10321 tatcaacgtg aacggtgttg atcagctgac gtgcggaaga catcagtttg ctccagaagt
    10381 cctggtggtg accacaaacc accagatcca tatcgtattt cttgattgca tcgaccagta
    10441 cctggcccag atcgccgctg ccgctcaggg tttcagtgat tgggtagcct gcattggttg
    10501 aaagctcggt cagtgcatga tgtgtctctt cagagatgcg tttctgcata tcacccagat
    10561 tcacatcgat aagcccggtg tataggtcag agtagtttac atctacgtgg atcagagaaa
    10621 ctttcgcatt gtaggggcga gccatagaga ctgctttctc taccagaact ttgctttccg
    10681 gggagaggtc gaccgcgatg agaatgtgtt tataagccat agtgttactc cttccataaa
    10741 gttgtcgatg actggccagc tagcgtttct tgtgccgtta cgtcacccgc gccctgcgaa
    10801 ctacgtgcgc ggggctaacc cagccgtaaa gattcagtgt taagaccatc cttaccttat
    10861 agcgacccgg atgatccgtc aatccgcctt gcttactcgt tagataaaca aattttcgac
    10921 atgaatatat tgatagtggt taaccttctg gaaaaaaaac aacctgatct cctacactat
    10981 ctatagagcc gctcggatgt tggtcaccac aaaagcggtt tctccaggac ctggtgttaa
    11041 ctgattggca gcgtatcggg agcggtagcc ccggggtgga actccgcggg caggtcgccg
    11101 gggaggaggt atgataagca ccgtcgcatt attttgggct ttatgtgtcg tttgcattgt
    11161 taacatggcg cgctatttct catcactacg cgcgttgtta gtggtactgc gtaactgcga
    11221 tccattgctc tatcaatatg ttgatggagg gggctttttt acctcacatg gccaacccaa
    11281 caaacaggtg cgtctcgttt ggtatatcta tgcccaacgt tatcgcgatc atcacgatga
    11341 tgagtttatt cgccgctgtg agcgggtgcg tcggcagttt attctgacca gcgcattgtg
    11401 tggtctggtg gtggtcagcc tgattgcatt gatgatttgg cattaagcaa ttcagcaata
    11461 atgacgcata aaaaaagcgg gccagtaaac tgacccgctc gttttggtgc gtacaattac
    11521 aggaactgca aggagagcca gtacagcccg ccggaaagca gtaccgcagc cggaagggta
    11581 aacacccagg ccatcagaat actggttacg gttttacgct gtaagccgcc gccatctacc
    11641 accatcgtcc ccgcgacaga agaggagagt acgtgagtag tggaaaccgg catcccggta
    11701 taacttgcca ggccgataga cacagccgcc gtcatctggg cagacatccc ctgagcgtag
    11761 gtcatgcctt tcttaccgat tttctcaccg atagtcgttg ccacacgacg ccagccaatc
    11821 atcgtaccga tacctaacgc cagcgcgacc gccatgatga tccacaccgg cgcatactcg
    11881 atggtgctaa gcatgtcgga cttcagtttc ttcaacaggc gctgatcgtc agcactcacg
    11941 ccaggcatct tcaccacttt atcgatagtg tcagaaacgc acagcataat gcggcgcatc
    12001 tggctacgtt gatcaagcga cagcttgtcg tagctttcca catcggtagt caacatgcct
    12061 ttcaggcggt tgagcgcgtt aatggtattc gacggatggc aatggaactc cgcgggttgc
    12121 gttgcgccag cttccggagc cggtactaac tgatcagcac cggtcgcttg tttcagcagc
    12181 gcaggatgct gctcaaagta agcttcgacg ttgttgatgg catcacgggt acgggtgatt
    12241 tcgtagccag tggcattcat gttcaccacg aagcctgctg gtgcgacacc aatcaatacc
    12301 aacataacca gaccaatgcc tttctgacca tcgttcgcgc cgtgcgaaaa cgccacgccg
    12361 acagcggaaa ggatcagcgc aatacgcgtc cagaacggcg gctttttctt gccgtctttc
    12421 ttttcacgct ccgctggggt caggtggata cgggcgcgtt tcttggtgcc gctccagtag
    12481 cgacgcagca agaaaatcag accgccagca aacaccaggc cgacaatagg ggaaacgatc
    12541 agagaaccga aaatacttaa tactttcggg atattgagtg catccaccac tgacgtcccg
    12601 gtcatcaacg cattggttaa accaatcccg atgatcgcgc caatcagcgt atgagagctg
    12661 gatgcaggta aaccaaagta ccaggtaccc aggttccaga taatcgccgc cagcaacata
    12721 gagaacacca tggcaaggcc atgagacgat cccatattaa gcagcagatc cgtcggcagc
    12781 atatgcacaa tggcataggc aacactcaga ccacccagca aaacacccaa aaagttgaat
    12841 accgccgcca taaccacggc gagctgagaa cgcatcgcgc gggtatagat aacggttgcc
    12901 acggcgttgg ctgtgtcatg gaaaccattg atggcttcgt agaacagcac aaaagccagt
    12961 gcaagcaata ataacagccc ggtatgcaaa tccaggccag caaacaaatg tagcatagga
    13021 cgttacgcca ttttgaggac atgaacgcgg cgcattatca gtgactttcg cggcgcgggc
    13081 aaagtgaaat atagactttt tttgatttgc ctcctgtatg gattccactc aaaaaataat
    13141 tatcttatat aattcaggca aatacttcct tttagtaata ttgatgctgg tgcgaccact
    13201 gaggaatctt tacaattcac gcccgttttt tcttagagga gcgcaacgtg gaaaggtttg
    13261 atgccattat tataggcgct ggtgcggcag gtatgttctg ttctgcgctg gcaggtcagg
    13321 caggacgccg ggttctgctg atcgataatg gtaaaaaacc agggcgcaaa atcctcatgt
    13381 ctggcggtgg gcgctgcaac tttaccaacc tttatgtcga accgggcgct tatctgagcc
    13441 agaatccgca tttttgtaag tctgcactcg cgcgttttac ccagtgggat ttcattgatc
    13501 tggtcaataa acacggcatc gcctggcacg agaaaacgtt agggcagctc ttctgcgatg
    13561 actccgcgca gcagattgtc gacatgctgg tggatgagtg cgagaagggc aatgtggcct
    13621 tcagattgcg tagcgaagtg ctgagcgtgg cgaaggatga tacaggcttc acgcttgaac
    13681 tgaacggcat gactgtcggt tgcgaaaagc tggtcattgc gaccggtgga ctgtcaatgc
    13741 cggggctggg cgcatcgccg tttggttata agattgccga acaattcggc ctcaacgtgc
    13801 tgccgacccg tgcgggtctg gtgccattca ctctgcataa accgttgctt gaagagttac
    13861 aggtgctggc gggcgtggcg gtgccttccg tgattaccgc tgaaaacggt accgttttcc
    13921 gtgagaactt actcttcacc caccgcggct tatctggacc ggcggtgttg cagatttcca
    13981 gctactggca accgggtgaa tttgtcagca tcaatctgct accggatgtg gacctcgaaa
    14041 ccttcctgaa tgagcagcgt aatgcacatc cgaatcaaag cctgaaaaac acgctggcgg
    14101 ttcatctgcc gaagcggttg gttgaacggt tacaacaact cgggcaaatc ccggatgtct
    14161 cgctaaaaca gctcaacgtg cgtgaccaac agacactgat tagcacattg actgactggc
    14221 gcgtacaacc caacggcact gaaggctatc gcactgccga agtgacgctc ggcggcgtgg
    14281 acaccaacga actctcttca cggacgatgg aagcgcgcaa agtgcctggg ctgtacttca
    14341 tcggcgaagt gatggacgtc accggctggc tggggggcta taacttccag tgggcgtggt
    14401 cgagtgcatg ggcttgtgcg caggatttgg tggcagaata agaagcaaaa tatgctctcg
    14461 ataccagagg gattaacctg aataaaacca gataaggcgt ccaacgcctt atctgaagcc
    14521 ttttttatta ttttttcgct gaacccgctt ctaatataga aacgatggaa aacagtgtaa
    14581 aacaaactgc acctaagcca accagaactc gcgaaggaat aaagtaagcc atatcggttt
    14641 gcgcaatttc agccaggaac gcagcaagga acagacaaaa cagacaggta aataccggaa
    14701 tcatgggtat tctgttggct aacgaacagg tgcgtcgcca taccagcgct aacaaccaca
    14761 ctttggagaa aatactgtag cagatcatcc ccaggcaaat gagaataatg ccgggggcca
    14821 ggcgggcgct tgcatctgaa ctgattaata cgtagatacc aaaaataatg gtaagtgagc
    14881 caagcaaaat aacccaatag caccacagcc aatgttcttt cactgagaac gtattccgcg
    14941 tttgatggac aatagtggcg acaaggccaa taagacaagc gcaaattgcc gttaacccca
    15001 ttagtacgtg acctgcaaca taatgtggtg tgatatctgc actacgtaac agtatgactg
    15061 accaaatgaa accaaagaga gtaagcagaa cgggaacggc aattaagcaa ttaccgattg
    15121 ttgaagaata ggcctgcaat ggcgtaccgt cactcttact gcccgaggcg tttttgggga
    15181 tcaacagaaa atgtccggaa gatgccgcta cggttgatac acaagcggca atcatgccaa
    15241 cgccgaaaat aacatgaccg gcgacaaatt catctgccat cacattattg cttgccaaca
    15301 gtgaccatcc ccaaatcata gttgtagcag aaccggcata accaataaca ggaaataacc
    15361 tgttgtagaa tttattcatg ccgtgagtta actgagatat aataataaat gcggtagtga
    15421 acaatgccag acatatcgca gaaagagaaa tcagaacatg ccctgcaacg aaatattcac
    15481 tttgcccaga accggttaat acaaactcac ctaatgcgat gcatattata cccatcagta
    15541 aaggaattaa tttaaacaac catccgatat atatattcat tttaaacccc atatatattt
    15601 tctgctacac ttcgaaaaac atatgattcg tcttttcagt tagttaaata tgtctataag
    15661 atgccatcaa ttaaaaaaag taacttcact ataatcggat ttagcgttat ggggatcaac
    15721 ttt
//
LOCUS       DAZFFE010000108        15455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFFE010000108 DAZFFE010000000
VERSION     DAZFFE010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.108"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..125
                     /locus_tag="ACHJ69_003806"
     CDS             <1..125
                     /locus_tag="ACHJ69_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016695.1"
                     /translation="TYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHIIG"
     gene            143..541
                     /gene="yjfL"
                     /locus_tag="ACHJ69_003807"
     CDS             143..541
                     /gene="yjfL"
                     /locus_tag="ACHJ69_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO5016696.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAAALAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            551..1189
                     /gene="yjfM"
                     /locus_tag="ACHJ69_003808"
     CDS             551..1189
                     /gene="yjfM"
                     /locus_tag="ACHJ69_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO5016697.1"
                     /translation="MARKRKSRNNKNIGHGAISRIGRPHNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNSDICAHGWNNAKAAFYADV
                     PKNMTQHNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            1192..2355
                     /gene="yjfC"
                     /locus_tag="ACHJ69_003809"
     CDS             1192..2355
                     /gene="yjfC"
                     /locus_tag="ACHJ69_003809"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO5016698.1"
                     /translation="MLRHNVPVRRDLDRIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGVIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFDGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2439..4064
                     /gene="aidB"
                     /locus_tag="ACHJ69_003810"
     CDS             2439..4064
                     /gene="aidB"
                     /locus_tag="ACHJ69_003810"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO5016699.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGASWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTSAERLDDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGIPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCKVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(4181..4456)
                     /gene="yjfN"
                     /locus_tag="ACHJ69_003811"
     CDS             complement(4181..4456)
                     /gene="yjfN"
                     /locus_tag="ACHJ69_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO5016700.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4605..4934)
                     /gene="bsmA"
                     /locus_tag="ACHJ69_003812"
     CDS             complement(4605..4934)
                     /gene="bsmA"
                     /locus_tag="ACHJ69_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO5016701.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            5116..5865
                     /gene="yjfP"
                     /locus_tag="ACHJ69_003813"
     CDS             5116..5865
                     /gene="yjfP"
                     /locus_tag="ACHJ69_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5016702.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFSVALAQAGLRVIMPDAPDHGSRFSGDAAWRLKQFWQILLQSMQEFTTLRAAIAEEN
                     WLRDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFHQHL"
     gene            complement(5862..6617)
                     /gene="ulaR"
                     /locus_tag="ACHJ69_003814"
     CDS             complement(5862..6617)
                     /gene="ulaR"
                     /locus_tag="ACHJ69_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO5016703.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6725..7789)
                     /gene="ulaG"
                     /locus_tag="ACHJ69_003815"
     CDS             complement(6725..7789)
                     /gene="ulaG"
                     /locus_tag="ACHJ69_003815"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO5016704.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGANVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GTLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADILRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            8144..9541
                     /gene="ulaA"
                     /locus_tag="ACHJ69_003816"
     CDS             8144..9541
                     /gene="ulaA"
                     /locus_tag="ACHJ69_003816"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO5016705.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIERMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9557..9862
                     /gene="ulaB"
                     /locus_tag="ACHJ69_003817"
     CDS             9557..9862
                     /gene="ulaB"
                     /locus_tag="ACHJ69_003817"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO5016706.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            9872..10336
                     /gene="ulaC"
                     /locus_tag="ACHJ69_003818"
     CDS             9872..10336
                     /gene="ulaC"
                     /locus_tag="ACHJ69_003818"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO5016707.1"
                     /translation="MKLRDSLAENKSIRLQTEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10350..11000
                     /gene="ulaD"
                     /locus_tag="ACHJ69_003819"
     CDS             10350..11000
                     /gene="ulaD"
                     /locus_tag="ACHJ69_003819"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO5016708.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIGQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            11010..11864
                     /gene="ulaE"
                     /locus_tag="ACHJ69_003820"
     CDS             11010..11864
                     /gene="ulaE"
                     /locus_tag="ACHJ69_003820"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO5016709.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGMVEAA"
     gene            11864..12550
                     /gene="ulaF"
                     /locus_tag="ACHJ69_003821"
     CDS             11864..12550
                     /gene="ulaF"
                     /locus_tag="ACHJ69_003821"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO5016710.1"
                     /translation="MQKLKQQVFEANMDLPHYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12680..12955)
                     /gene="yjfY"
                     /locus_tag="ACHJ69_003822"
     CDS             complement(12680..12955)
                     /gene="yjfY"
                     /locus_tag="ACHJ69_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO5016711.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            13283..13678
                     /gene="rpsF"
                     /locus_tag="ACHJ69_003823"
     CDS             13283..13678
                     /gene="rpsF"
                     /locus_tag="ACHJ69_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO5016712.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13685..13999
                     /gene="priB"
                     /locus_tag="ACHJ69_003824"
     CDS             13685..13999
                     /gene="priB"
                     /locus_tag="ACHJ69_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO5016713.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            14004..14231
                     /gene="rpsR"
                     /locus_tag="ACHJ69_003825"
     CDS             14004..14231
                     /gene="rpsR"
                     /locus_tag="ACHJ69_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO5016714.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14273..14722
                     /gene="rplI"
                     /locus_tag="ACHJ69_003826"
     CDS             14273..14722
                     /gene="rplI"
                     /locus_tag="ACHJ69_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO5016715.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            15124..>15455
                     /locus_tag="ACHJ69_003827"
     CDS             15124..>15455
                     /locus_tag="ACHJ69_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5016716.1"
                     /translation="MGKKTAPLLPYTNQMLSEFGERLKLARLRRRLTAKQVAERAGMS
                     PITLRSLESGSAGVTIGAYLSVMQVLGLETDLNKLAAEDDLGRQLQDSRLIKKRGLSR
                     SSGKNEEG"
BASE COUNT         3661 a         3728 c         4128 g         3938 t
ORIGIN      
        1 atacttacga atatttgctg ttaaatggtg aggaatcttt taacgatctc ggcgagccgg
       61 agtggttatt ttcgcgcgcg ctgggggtcg atatcccgct gacatcactt catattattg
      121 gttaattaca aggacgttta caatgcatat actggattca cttcttgcct ttagtgccta
      181 tttttttatt ggcgtggcga tggtgattat tttcctgttt atctactcca aaattacacc
      241 gcacaacgaa tggcagttaa tcaaaaacaa taacaccgcg gcggcactgg cgttcagcgg
      301 aacattgttg gggtacgtga tccccttatc cagtgcggca atcaatgcgg tgagtattcc
      361 agactatttc gcatggggcg ggattgctct ggtgattcaa ttactcgttt ttgctggcgt
      421 caggctttat atgcccgcat taagcgaaaa aattattaat cacaataccg cagcaggaat
      481 gtttatggga accgccgcgc tggctggcgg tatttttaat gcagcttgta tgacatggta
      541 atggacgatc atggccagaa aacgcaaatc cagaaataac aaaaatatcg gacatggggc
      601 aatcagtcgc attggtagac cgcataatcc ttttgagccg cgccgcaatc gctacgcaca
      661 aaaatactta acgttggcgc taatgggcgg tgccgctttt ttcgtattga aaggttgtgg
      721 cgatagtggc gatgtcgata acgacggcga cggaacgttt tacgcgactg tgcaggattg
      781 tattgatgat ggtaataatt cagatatctg tgctcatggc tggaacaacg ccaaagcggc
      841 attttatgcc gatgttccga agaatatgac tcagcataat tgtcagtcta agtacgaaaa
      901 ttgctactac gacaatgttg aacagagttg gatcccggtc gtttctggat ttttgttaag
      961 ccgggttatt cgtaaagatc gcgatgagcc gttcgtctat aacagcggcg gatcctcatt
     1021 tgcttcccgc cccgtctggc gcaacacttc cggtgattac tcctggcgct ccggttctgg
     1081 caaaaaagag tcttactctt cgggcggctt taccaccaaa aaagcgtcaa ccgtttctcg
     1141 cggcggctat ggtcgttctt ccagcgcccg tgggcattgg ggaggctaat catgctgaga
     1201 cacaacgttc ctgtgcggcg ggatctggac cggattgccg ccgataacgg atttgacttt
     1261 catatcatcg acaatgaaat ctattgggat gagagtcggg cttatcgttt tactctgcgc
     1321 cagattgaag agcagatcga aaaaccgact gcggaactgc atcagatgtg cctggaggtg
     1381 gtggatcgcg cggttaaaga tgaagagatc ctgacgcaac tggcgatccc gccgttgtac
     1441 tgggatgtga tcgccgaaag ctggcgcgca cgcgatcctt cgttgtatgg ccggatggat
     1501 tttgcctggt gtggtaatgc accggttaag ttgctggagt acaacgccga tacgccaact
     1561 tcattgtacg agtcggctta tttccagtgg ttgtggctgg aagatgcccg gcgcagcggc
     1621 gttattccgc gtgatgccga tcagtacaat gccattcagg aacgcctgat ttcacgtttt
     1681 agcgagcttt acagtcggga accgttttat ttttgctgct gtcaggacac cgacgaagac
     1741 aggagtaccg tgctgtactt gcaggactgc gcccagcagg cagggcagga gtcgcggttt
     1801 atctacattg aagatctcgg tttgggcgtc ggcggcgtac tgaccgatct tgatgataat
     1861 gtcatccagc gtgcatttaa gctgtatccg ctggagtgga tgatgcgtga cgataacggc
     1921 ccgctgctgc gcaagcgtcg tgagcaatgg gtggagccgt tatggaaaag tatcttgagt
     1981 aataaagggc taatgccgct gctttggcgc ttcttccctg gtcatcctaa ccttcttgca
     2041 tcctggttcg atggtgaaaa accgcagatt gccgctggcg aaagctatgt gcgtaaaccg
     2101 atttactcgc gcgaaggcgg aaacgtcacc attttcgacg gtcagaataa cgtcgttgac
     2161 cacgctgatg gtgattacgc cgatgaaccg atgatctacc aggcgtttca acctctgccg
     2221 cgatttggcg atagctacac actcatcggt agctggattg tcgatgatga agcgtgcgga
     2281 atggggatcc gtgaagataa cacgctgatc accaaagaca cctcacgttt cgttccgcat
     2341 tacattgctg gataagaatg ttttagcaat ctctttctgt catgaatcca tggcagtgac
     2401 catactaatg gtgactgcca ttgatggagg gagacacagt gcactggcaa actcacaccg
     2461 tttttaatca acctatacca ttaaataaca gcaacttata cctgtctgat ggcgcgcttt
     2521 gcgaagcggt aacgcgtgaa ggtgctagct gggatagcga ttttctcgcc agtattggtc
     2581 agcagttagg aacggctgaa tcccttgaac tggggcggct ggcgaatgtg aatccgcctg
     2641 aattattgcg ctacgatgcg caaggacgcc gtctggacga tgtgcgtttt caccccgcct
     2701 ggcacctgct gatgcaggcg ctatgtacca atcgggtgca caatcttgcc tgggaagaag
     2761 acgctcgctc cggcgcattt gtggcgcgcg cggcgcgttt tatgttacat gcgcaggttg
     2821 aggctgggtc gttatgtccg ataacgatga cctttgccgc cacgccattg ttgttacaga
     2881 tgttacccgc gccgtttcag gactggacca cgccgctgct gagcgatcgc tacgattctc
     2941 acttattgcc aggtgggcaa aaacgtggct tgttgatcgg catgggaatg acggaaaagc
     3001 agggcggttc cgatgtcatg agcaacacca ccagcgcaga gcgtctggac gatggttctt
     3061 atcggctggt ggggcataaa tggtttttct cggtgccgca aagcgatgcg catctggtgc
     3121 tggcacagac cgcgggcggt ttgtcctgct tttttgtgcc gcgctttttg cctgacgggc
     3181 aacgcaacgc gattcgcctc gagcggctga aagataagct gggtaatcgc tctaacgcca
     3241 gctgcgaagt ggagtttcag gatgccattg gctggttgtt ggggcaggaa ggggaaggaa
     3301 ttcgtctgat cctgaaaatg ggtgggatga cgcgttttga ttgcgccctg ggtagccatg
     3361 ccatgatgcg ccgtgcattt tcgctggcga tttaccatgc acatcaacgc catgtttttg
     3421 gtaatccatt gatccaacag cctcttatgc gtcatgtctt aagtcgcatg gcacttcagc
     3481 ttgaagggca aacggcgttg ctgtttcgtc ttgcgcgagc gtgggaccgg cgcgccgatg
     3541 ccaaagaagc tctgtgggcg cgtttattta cgcctgcggc gaaatttgtg atctgcaaac
     3601 gaggcattcc gtttgtggcc gaagcgatgg aggtgctggg cggcattggt tattgcgagg
     3661 agagcgagct gccgcggctt taccgggaga tgccggtaaa cagtatttgg gaaggttccg
     3721 gcaatattat gtgcctggat gtgctgcgcg ttctcaataa gcaagcgggc gtatacgact
     3781 tattgtcgga agcatttgtg gaagtgaaag ggcaggatcg ctattttgat cgcgcggttc
     3841 gtcgtttaca gcagcagctg cgtaagccag ctgaagaatt ggggcgagag attactcatc
     3901 agctattcct gctgggctgc ggtgcgcaaa tgttgaaata tgcctctccg ccaatggcgc
     3961 aggcgtggtg taaggtgatg ttagatacgc gcggcggcgt acggttgtca gagcagatcc
     4021 agaatgattt attgctgcgg gcgacgggag gagtgtgttt gtaagcgtat acgactgatg
     4081 cgacgctggt ttcgattaac taaatgaaat attttaaaat tgtaggccgg acaaggcgct
     4141 cgcgccgcat ccggcattgt tcatttgctg tagatgacac tcacgcatac agtatcgcct
     4201 gtacgcgcca gttatcaggc tgctggtctt cgtacatggt aataatgcgg taccatgacg
     4261 caccttgttc atcggccttt aacgccacga tacgttccac gtcctgcgga tcccctgaaa
     4321 tattgctaac tgagatctga cctatttcat tcaggcccgt cacgcagtca gcactggcga
     4381 attctgcaga ctgtgccgtg gcgctcgcag gataagtcac taattgcagc gagggtgagg
     4441 caagtaattg tttcatcgtc agctccattt ttcgtttccc cacatgttgc agggcattcc
     4501 atcacggtaa taaacgcccg agcttccagc cggtaattta ttgtgcacag gtaaacgcat
     4561 agggacgcaa agcaatgtaa agctgactat gtaagttgtc tgatttattt acggtacaaa
     4621 atggcttgtg aataccactg tcctgtcacg atggtttcgt ccaccatgac gacaacgtaa
     4681 taatcagctt ttgcagcgac agctttcgct ctgatttctg ctaatgcgtc atccggagaa
     4741 ccccgaacca tcgtgcttac gctacctatt cgctgtaacc cttgcgtctg gtcgcggcga
     4801 atctcttgcg gatggtccgt tactggcggt gctggctgtg gcgtaccttg cagtgcgcta
     4861 caggcactta acatcaacac caataataaa ctggcaaacc ggtaaataac gctattacgt
     4921 ttcctgctaa ccatagtgta gtgccttatt aatttaactt tgggggaatg ttcccgaatt
     4981 gttacctggt acgcgatttt cgaatgaaaa cgtgacgaaa gcgtaaccca catctcaaca
     5041 ttatgaacta ataccgccag cagaaacttg tgctaaggat ttcttcatgt cataccagac
     5101 aaaaaggaga gacagatgat tgaaatagaa tcacgcgagc tggcagatat tcccgttctt
     5161 catgcttatc ctgtcgggca aaaagatacc ccgttaccgt gtgtgatttt ttatcacggc
     5221 tttacttcgt ccagtctggt gtatagctat ttttccgttg cgctggcgca ggctggtttg
     5281 cgggtgatca tgccggatgc gcccgatcac ggtagccgtt ttagtggcga cgcagcgtgg
     5341 cggttaaagc aattctggca aatcttgcta caaagtatgc aggaattcac tacattacgt
     5401 gcggcaatag ccgaagaaaa ctggctgcgt gatgaccgtc tggcagtcgg tggcgcgtcg
     5461 atgggcgcga tgacggcact gggaattacc gctcgccacc ccacggtaaa atgtaccgcc
     5521 agcatgatgg gatcgggcta ttttacatcg ctcgcccgtt cactgtttcc accgctgata
     5581 cctgaaacgg cagcacagca gaatgaattc aataacattg tcgcgccgct ggcagagtgg
     5641 gaagcgacaa accacctgga acaacttggt gacagacctc tactgctgtg gcatggcctt
     5701 gacgatgacg tcgtgcctgc cgacgaatca ctacgtttgc agcaggcctt aagcgagacg
     5761 gggcgggata aactgctaac ctgttcatgg cagccaggcg tgcgtcaccg cattacgcct
     5821 gaggcgttag atgctgccgt gacatttttc caccagcatc tttaaacacg cagaatgctg
     5881 accccttgcg cttccagttg ttgcaggatt tccgggttag catttttgcc ggtgatcagc
     5941 atatcgattt gatcggcacg gctaaaaagc attcccgcgc gttcgccaat cttactgcta
     6001 tcaaccagta ccaccagttt ccctaccacg ctcagcatct tctgctctgc cattgccgtc
     6061 agcatatcgg ttttatacaa cccttctgct gtcagtcctt tgccgctggt aaacatccag
     6121 tgcccggcat agagactgtt ttcgctgcct tgcgggctta aagtgatgga ctgacttttg
     6181 ttgtactgtc cgcccataat gatcacgctg tcatgctcct gatcgatcag gtaatttgcc
     6241 agcggtagat aattagtgat gatttgcact ggcttgccac acatttcccg cccaagcaga
     6301 aacgcggtgg agccgcagtt gatgactacg ctttcgcccg gattaaccag ctgcgaggcc
     6361 gctttagcaa tacgtacttt ttcatcgtga ttctgcgctt gatgcagatt catcggcgtc
     6421 cagcgcgggc gctgttgggt aatagcttct gcgccattgc gcactttttt cagtttgccg
     6481 ctttcgtcaa gtttattgat atcgcgtcgt gcagtggcag gcgaaattcc cagacgctca
     6541 acgactttct caacggtcac aaagcccaat tgtgcgagca tttccaggag gatttgatgt
     6601 ctttgtgctt cagtcatgag ctattccgat aaaatttgat ttttttagat gatatttgaa
     6661 atagccagga aatactatgc cggatagcgc gagcatctcc acgctatccg gtacaagcga
     6721 cagattacag gaacgacttg aacggcagat ctggttcaat agtgaagcaa tcatcgaaac
     6781 cacgcggata gtggtactcg aagttgtctt tatccagcgg ccaggtaaat ttgccgccga
     6841 cctgccagat aaacggcttg aagccatact tcaggcgatc ttttttcatc tcccacagca
     6901 cgcggatctc ttgcggatcg gcctggaagt ttgaccagat atcgtggtgg aacgggatca
     6961 ctactttcgc attcagcgct tcgcccatac gcaagatatc agcactggtc attttgtcgg
     7021 taataccgcg tgggttttcg ccgtatgaac ctaaagccac gtcgatctga tgctcattac
     7081 catgttttgc atagtagtta gagtagtggg aatcgccgct gtgatacagg gtaccgcctg
     7141 gggttttgaa cagatagttc accgcgcgat cgtccatgcc gtccggcagt acgccagctg
     7201 ctttttgatc ggcaggcagg gtgatcagtg ccgtgcggtc gaaagcgtca agtgcatgaa
     7261 tttcaatgtc ttttaccttc actacgtcgc ccggtttaac gacgatgcaa cgctctttcg
     7321 gtacgcccca gccaatccac aaatcaacgc aggttttcgg tccaatgaac ggtacgtcat
     7381 cagcacagtt ctgcatcacg gcagcagcga cgttaacgtc gatatgatcg ttgtgatcgt
     7441 gagtcgccag taccgcgtcg atctggcgaa tcgcaaacgg atcaagaaca aacggggtgg
     7501 tgcgcaggtt tggctgcagt tttttcacgc cagccatgcg ctgcatctgg tgaccctgtt
     7561 tcattaacgg gttaccgtgg ctttgtttgc cagtaccgca ccaaaaatca acacaaacgt
     7621 tggcaccgcc ttctgatttc aaccagatcc cggtacagcc gagccaccac attgcgaaag
     7681 tgccaggtgc gacctgctct tgctcaattt cttcattcag ccagctaccc cactccggga
     7741 aagtgctcag gatccaggac tcacgggtga tacttttcac tttactcatc gccatttacc
     7801 ttcgtgatag ttcaattcga atcaatatgt gattggtttt gattaatcct gacactattt
     7861 tttcaggaag gcaatgacca ttttttgact ttttagcagg agagttattg tggattttga
     7921 gtgtggaaag attaaattga atgtgtaatg caattaacta aatgaattta aatgaataaa
     7981 tatttcatta tgtgaatccc attctgttta tgtggaatta tttgcgtgcg acgtcacatt
     8041 taatcataaa taatcttgtt gtgattactt ttgaaaatta gagtgagagc acaacattcc
     8101 gggtgtgcga aatacccggt tacctcttct tcaggagatc gttatggaga tcctctacaa
     8161 catctttacc gtgtttttta accaggtcat gaccaatgcc ccgttgttgc tgggtattgt
     8221 gacctgtctg ggttacatcc tactgcgcaa aagtgtcagc gttattatta aaggtacgat
     8281 taaaaccata attggtttca tgttgttgca ggcagggtcc ggcatcctga ccagcacctt
     8341 caaaccggtg gtggcgaaaa tgtccgaagt ctacggcatt aacggcgcaa tttccgatac
     8401 ttacgcgtcg atgatggcaa ccattgaacg catgggcgat gcctatagct gggtgggtta
     8461 cgcggtgttg ttagcgctgg cgctgaacat ctgttacgtg ctgctgcgcc gaattaccgg
     8521 tattcgcacc atcatgctga ctggtcacat catgttccag caggcggggc tgattgccgt
     8581 tacgctgttt atcttcggct actccatgtg gacgaccatt atctgcaccg cgattctggt
     8641 ttcgctctac tggggcatta cctccaacat gatgtacaag ccgacacagg aagtcaccga
     8701 cggctgtggt ttctccattg gtcaccagca acagtttgca tcatggattg cctataaagt
     8761 tgcgccgttc ctcggcaaaa aagaggaaag cgttgaagac ctcaaactgc caggctggct
     8821 gaatattttc cacgacaaca tcgtctccac agcgattgtc atgactatct tctttggtgc
     8881 cattctgctc tcctttggta tcgacaccgt gcaggcgatg gcaggcaaag tgaactggac
     8941 ggtgtacatc ctgcaaaccg gtttctcctt tgcggtggcg attttcatca tcactcaggg
     9001 cgtgcgcatg tttgtggcgg agctctctga agcattcaac ggtatctccc agcgcctgat
     9061 cccgggtgcg gttctggcga ttgactgtgc ggctatttat agcttcgcgc cgaacgccgt
     9121 ggtctggggt tttatgtggg gcaccatcgg gcaactgatt gcggttggca tcctggtcgc
     9181 ctgtggctct tcaatcctga ttattccagg ctttatcccg atgttcttct ccaacgccac
     9241 tatcggcgtg ttcgctaacc acttcggcgg ctggcgcgcg gcgctgaaga tttgcctggt
     9301 gatggggatg atcgaaatct ttggttgcgt ctgggcggtg aaactcaccg gtatgagcgc
     9361 ctggatgggc atggcggact ggtcgattct ggcaccgccg atgatgcagg gcttcttctc
     9421 catcggcatc gcctttatgg ccgtcatcat tgttattgca ctggcttata tgttcttcgc
     9481 tggccgcgca ctgcgtgcag aagaagatgc agaaaaacaa ctggcagaac aatctgctta
     9541 ataaggagtt ttgattatga ccgtacgtat tctggctgtg tgtggtaacg gacaaggcag
     9601 ttccatgatc atgaaaatga aagtggacca gtttttaacc cagtcgaaca tcgaccacac
     9661 ggtaaacagc tgcgcggttg gcgaatacaa aagcgagttg agcggtgcgg atatcatcat
     9721 cgcttctacc catattgcgg gcgaaatcac cgtgaccggc aacaaatatg tggttggcgt
     9781 gcgcaacatg ctctctcctg ctgattttgg cccgaaactg ctggaagtga tcaaagagca
     9841 tttcccgcag gacgtgaagt aaggacgcgc catgaaatta cgtgattcgc tggctgaaaa
     9901 taaatctatc cgcctgcaaa cggaagcaga gacatggcag gacgcagtga aaatcggcgt
     9961 tgatctgctg gtagcggcag atgtggtaga gccgcgttac taccaggcga ttctggatgc
    10021 ggttgaacag cacggcccgt actttgtgct cgcacccggc ctggcaatgc cgcacggacg
    10081 cccggaagag ggggttaaga aaactggttt tgcgctggtg acgctgaaaa agccgctgga
    10141 gttcaaccac gaagataacg acccggtgga tatcctcatc accatggcgg cggttgatgc
    10201 caacactcac caggaagtgg gcatcatgca gatcgtcaac ctgtttgaag atgaagagaa
    10261 tttcgaccgt ttacgtgcct gccgtaccga gcaggaagta ctggatctca ttgaccgcac
    10321 caacgcggca gcttaagaag gaattgaaca tgtcattacc gatgttacaa gtcgcgctgg
    10381 acaaccagac tatggatagc gcctacgaaa ccactcgcct gattgccgaa gaagtcgaca
    10441 ttatcgaagt gggcaccatt ctttgcgtag gcgaaggcgt gcgtgcggtt cgtgacctga
    10501 aagcgctgta cccgcacaaa atcgtactgg ctgacgccaa aattgccgat gcaggcaaaa
    10561 tcctttctcg catgtgcttc gaagccaacg ctgactgggt gaccgtaatt tgctgtgcgg
    10621 atatcaacac cgccaaaggc gcgctggacg tggcaaaaga gtttaatggc gacgtgcaga
    10681 tcgaactgac cggctactgg acctgggaac aggcgcagca gtggcgcgac gcaggcattg
    10741 ggcaggtggt ttatcaccgc agccgtgacg cgcaggccgc aggcgtggca tggggcgaag
    10801 cggacattac cgcgatcaaa cgtctttccg atatgggctt caaagtcacc gtcaccggtg
    10861 gtctggcgct ggaagatctg ccgttgttta aaggcattcc gattcatgtg tttatcgctg
    10921 gtcgcagcat tcgtgatgcc gcttctccgg tggaggccgc acgtcagttc aaacgttcca
    10981 tcgctgaact gtggggctaa ggagcggata tgttgtccaa acaaatcccg cttggcatct
    11041 acgaaaaagc gctccccgct ggggagtgct ggctggaacg actgcaactg gcaaaaacgt
    11101 taggcttcga ttttgtcgag atgtcagtag atgaaacgga cgatcgcctt tcgcgcctcg
    11161 actggagccg cgagcagcgt ctggcactgg tgaatgcgat tgttgaaacc ggcgtgcgcg
    11221 taccgtccat gtgcctttct gctcatcgtc gtttcccgct gggaagcgaa gatgacgcgg
    11281 tgcgggcgca ggggctggag attatgcgta aagctatcca gttcgcccag gacgtcggta
    11341 ttcgcgtgat ccaactggcg ggctatgacg tttactatca ggaagccaat aacgaaacgc
    11401 gtcgtcgttt ccgtgacggc ctgaaagaga gcgttgagat ggcgagccgc gcgcaggtca
    11461 cactggcgat ggagatcatg gattacccat tgatgaactc catcagcaag gcgctgggct
    11521 acgcacacta tctcaacaat ccgtggttcc agctctaccc ggatatcggc aacctgtcgg
    11581 cgtgggataa tgacgtgcag atggagttgc aggctggaat cgggcacatc gtcgcggtgc
    11641 atgtgaaaga caccaaacca ggcgtcttca aaaacgtgcc gtttggcgaa ggcgtagtgg
    11701 atttcgaacg ttgcttcgaa acgctcaaac agagtggcta ttgcgggccg tacctgattg
    11761 agatgtggag cgaaacggcg gaagacccgg cggcagaagt agtgaaagcg cgtgattggg
    11821 tgaaagcgcg catggccaaa gcaggcatgg tggaggcggc ataatgcaaa agctgaaaca
    11881 gcaggtgttt gaagccaaca tggatttacc gcattacggg ttggtgacct ttacctgggg
    11941 caacgtcagc gctattgacc gtgaacgcgg gctggtggtg atcaagccca gcggtgtcgc
    12001 ctacgaaacc atgaaagcgg acgatatggt ggtggtcgat atgagcggta aggtggtgga
    12061 aggggagtat cgtccgtctt ccgacactgc gacgcatctc gaactctacc gtcgttatcc
    12121 gtcgcttggt ggtattgtcc atacccactc cactcatgcc accgcatggg cgcaggcggg
    12181 gctggcgatc ccggcgttag gcaccacgca cgccgattac ttctttggcg atatcccgtg
    12241 tacgcgtggg ttaagtgaag aagaagtgca gggcgaatac gagctaaaca ctggcaaagt
    12301 gattatcgaa acgctgggtg acgccgagcc gctgcatacg ccgggaattg tggtgtatca
    12361 gcacggaccg ttcgcctggg ggaaagatgc ccacgatgcg gtgcataacg cggtggtgat
    12421 ggaagaagtg gcgaaaatgg cgtggattgc gcgcagtatt aacccacaac tgaatcacat
    12481 cgacagcttt ctgatgaata aacacttcat gcgtaagcac ggtcctaacg cttattacgg
    12541 gcagaagtaa aacacgcgct gcggaaattt ccttcttcgg gagataacta gtctaattcc
    12601 gctgccgttt ttcaaaaaaa agccccctgc gaagggggca aagcaaacta tggcaatgtt
    12661 tcgttggtta tacctggtgc tagcgataaa tatccgcgct ggcgtgcata ttgccgttac
    12721 tccccggttc ccgcatcaga attacgtggt agtacgttgc gccttgcgca tctgtctctt
    12781 catttaatgc ctgacgtgct tcactctcag tggcgaagtt atgattgata taaatcacgc
    12841 ctaagctttg cacatcgtcc atatttctgg cctggtggtt attaatttca atggctgccc
    12901 atgtatttgc acttagcaaa agcacagcca gaagggctaa aacgcgactg aacatagata
    12961 cctcctcgac ggctgacttt gtgtgctctc ctcttgttgt gatcttctga tttaatttta
    13021 atcaatgata aagaagttga tggtgaccat ttctgatgca gttgttcaaa aaaacaccat
    13081 gatgaagcgt gatgaactcc aaatcagcga tttagaggtt atttgcgaaa ggggcgattt
    13141 atttctgctc agcccttgag tttagcgggg tatgtcagta ttataacgcg tcatttttca
    13201 gccgaccttt aacacgttcc ttgcctcccc gggattcggc tgacccagac aggaggctga
    13261 ataatccgta aggagcaatt cgatgcgtca ttacgaaatc gtttttatgg tccatcctga
    13321 ccagagcgaa caggttccgg gcatgatcga gcgctacact gctgccatca ctggtgcaga
    13381 aggcaagatc caccgtctgg aagactgggg ccgccgtcag ctggcttacc cgatcaacaa
    13441 actgcacaaa gcacactacg ttctgatgaa cgttgaagct ccgcaggaag tgatcgatga
    13501 gctggaaact accttccgct tcaacgatgc cgttatccgc agcatggtta tgcgtaccaa
    13561 gcacgctgtt accgaagcat ctccgatggt taaagcgaaa gacgagcgcc gtgagcgtcg
    13621 cgatgatttc gcaaacgaaa ccgctgatga tgctgaagct ggggattctg aagagtaatt
    13681 tctgatgacc aaccgtctgg tgttgtccgg caccgtgtgc aggactcccc ttcgaaaggt
    13741 cagtccatca ggaattcctc actgccagtt cgtgcttgag catcgttctg tgcaggagga
    13801 agccggcttt caccggcagg catggtgcca aatgcccgtt attgttagcg gacacgaaaa
    13861 ccaggccatt actcacagta taacggtcgg cagtcgcata accgttcagg ggttcatttc
    13921 atgccataag gcaaagaacg gactgagcaa aatggttttg catgccgagc agattgaatt
    13981 gatagattct ggagactagc catatggcac gttatttccg tcgtcgcaag ttctgccgtt
    14041 tcaccgcgga aggcgttcaa gagatcgact ataaagatat cgctacgctg aaaaactaca
    14101 tcaccgaaag cggtaagatt gtcccaagcc gtatcaccgg tacccgtgca aaataccagc
    14161 gtcagctggc tcgcgctatc aaacgcgctc gctacctgtc cctgctgccg tacactgatc
    14221 gccatcagta atcggtcacg gtccattaat acgactttga gaggataagg taatgcaagt
    14281 tattctgctt gataaagtag caaacctggg tagcctgggt gatcaggtaa acgttaaagc
    14341 gggctatgct cgtaacttcc tggtaccgca gggtaaagct gttccagcta ccaagaaaaa
    14401 cattgaattc ttcgaagcac gtcgcgctga actggaagct aaactggctg aagttctggc
    14461 agcagctaat gctcgcgctg agaaaatcaa tgcactggaa actgttacca tcgcgtctaa
    14521 agctggcgac gaaggtaaac tgttcggttc catcggtact cgcgacatcg ctgacgctgt
    14581 aactgcagct ggcgttgaag tggctaagag cgaagttcgt ctgccgaacg gcgttctgcg
    14641 taccactggc gaacacgaag tgagcttcca ggttcacagc gaagtattcg cgaaagtgat
    14701 cgtaaacgta gtagctgaat aattcgttat tcaacgagac gtaaaaacgc cgaccagtgg
    14761 tcggcgtttt gcttttaagt gataaaaacc gcagaaacag acgttccctc cataaatctc
    14821 ttgctattcc ttttataaaa agttcgcagt acccatacct ggcccccagc ctcgccgctg
    14881 gtagttcgtg tcatgttttt tacgcgtaaa acacgacaaa agcatcaata cagcgatcgt
    14941 ctcacttgta tgataataaa aacaatatgt tatatatgaa tgtgctgtta ttttattgtt
    15001 tttatatata aaaagataaa atatcatcac tttcgtaatc ttgaacgctt tgcaggtgcg
    15061 ttcaatccaa taaatgctaa tattatggct atcattgcca taaaaagata aaatattatc
    15121 actatgggta aaaaaacagc accgttactc ccttatacga atcagatgct tagtgaattt
    15181 ggtgagcggc tgaaacttgc tcgtttacga cgcaggctta ccgcaaagca agttgctgaa
    15241 cgtgctggga tgtcgccaat aacattgcgc tctctggagt caggaagtgc aggggtcacg
    15301 ataggggcat atttgtctgt gatgcaggtt ttgggcctgg aaacggattt aaataagctt
    15361 gcagctgaag acgacttagg gcgtcagctc caggattccc gtttgattaa aaaaagaggg
    15421 ctttctcgtt catcaggtaa aaatgaagaa ggtat
//
LOCUS       DAZFFE010000109        15262 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFFE010000109 DAZFFE010000000
VERSION     DAZFFE010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.109"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            365..1189
                     /gene="gadX"
                     /locus_tag="ACHJ69_003828"
     CDS             365..1189
                     /gene="gadX"
                     /locus_tag="ACHJ69_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO5016717.1"
                     /translation="MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQV
                     DKCVENFVLVSRDTLSLFLPMLKEEALKLHVHKKVPSLLVHHCTRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQELPNCGSAASMAAQGIFYGTDRSAEGIRL"
     gene            1558..2286
                     /gene="gadW"
                     /locus_tag="ACHJ69_003829"
     CDS             1558..2286
                     /gene="gadW"
                     /locus_tag="ACHJ69_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO5016718.1"
                     /translation="MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFC
                     SLDTRRLDIEELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPIQLVTAIFDSFNDPR
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFSQHSPGTFS"
     gene            complement(2649..5762)
                     /gene="mdtF"
                     /locus_tag="ACHJ69_003830"
     CDS             complement(2649..5762)
                     /gene="mdtF"
                     /locus_tag="ACHJ69_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO5016719.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     ISATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIK
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKNNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVIGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPVEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5787..6896)
                     /gene="mdtE"
                     /locus_tag="ACHJ69_003831"
     CDS             complement(5787..6896)
                     /gene="mdtE"
                     /locus_tag="ACHJ69_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO5016720.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAEAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYGQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7004..7333
                     /locus_tag="ACHJ69_003832"
     CDS             7004..7333
                     /locus_tag="ACHJ69_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016721.1"
                     /translation="MRDLQTSGIVGLSASKMGCKHSDRHGEVNDKKNVAVLPTVSASI
                     RATKGSTGGYTYQGNKVVFVSPSIITGGIEISICIPRHASHQFQLLMSLLKIRCDTQG
                     DDVAHTS"
     gene            complement(7283..7810)
                     /gene="gadE"
                     /locus_tag="ACHJ69_003833"
     CDS             complement(7283..7810)
                     /gene="gadE"
                     /locus_tag="ACHJ69_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO5016722.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8609..9181)
                     /gene="hdeD"
                     /locus_tag="ACHJ69_003834"
     CDS             complement(8609..9181)
                     /gene="hdeD"
                     /locus_tag="ACHJ69_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO5016723.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKKQ"
     gene            9436..9768
                     /gene="hdeA"
                     /locus_tag="ACHJ69_003835"
     CDS             9436..9768
                     /gene="hdeA"
                     /locus_tag="ACHJ69_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO5016724.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKKASFKDKVKGE
                     WDKIKKDM"
     gene            9884..10210
                     /gene="hdeB"
                     /locus_tag="ACHJ69_003836"
     CDS             9884..10210
                     /gene="hdeB"
                     /locus_tag="ACHJ69_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO5016725.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10274..10921
                     /locus_tag="ACHJ69_003837"
     CDS             10274..10921
                     /locus_tag="ACHJ69_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO5016726.1"
                     /translation="MTAEFIIRLILAVIVCGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLGHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(10973..11743)
                     /locus_tag="ACHJ69_003838"
     CDS             complement(10973..11743)
                     /locus_tag="ACHJ69_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFO5016727.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(11740..12732)
                     /gene="shuU"
                     /locus_tag="ACHJ69_003839"
                     /gene_synonym="chuU"
     CDS             complement(11740..12732)
                     /gene="shuU"
                     /locus_tag="ACHJ69_003839"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFO5016728.1"
                     /translation="MLKDSLSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(12781..13404)
                     /gene="chuY"
                     /locus_tag="ACHJ69_003840"
     CDS             complement(12781..13404)
                     /gene="chuY"
                     /locus_tag="ACHJ69_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFO5016729.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVNRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(13404..13898)
                     /gene="hutX"
                     /locus_tag="ACHJ69_003841"
     CDS             complement(13404..13898)
                     /gene="hutX"
                     /locus_tag="ACHJ69_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFO5016730.1"
                     /translation="MSHVSLQEFLKTEPDGTLEAIAEQYNTTLLEVVKNLPSPTVVSG
                     DKFDTVWDTVCEWGNVTTLVHTTDVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAVSL
                     KEHA"
     gene            complement(13911..15248)
                     /gene="hutW"
                     /locus_tag="ACHJ69_003842"
     CDS             complement(13911..15248)
                     /gene="hutW"
                     /locus_tag="ACHJ69_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFO5016731.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNDDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGIETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIALEKP"
BASE COUNT         4055 a         3588 c         3613 g         4006 t
ORIGIN      
        1 tcacgttgta aaaaccgaat gcccaacctt taaaaaacag gtggggtatt cggctattta
       61 ttaattgcaa taatatatta cctgtctatt catcgtgttg ataagatata tgttatatga
      121 atgttatgga gcatagcgat actgcttaat attacgatga tgaattattt gcttgctatt
      181 aattcctctg cgttcttaag tccccttttt atataataaa tccagtcatc cagcccgagt
      241 atcatcgctc attatcatcc tgctttcccg cttcgtttaa atttatttat caatcaattt
      301 gacttaagag ggcggcgtgc tacattaata aacagtaata tgtttatgta atattaagtc
      361 aactatgcaa ccactacatg gtaattgtct aattgcgtac gcaagacata aatatattct
      421 caccatggtt aatggtgaat atcgctattt taatggcggt gatctggttt ttgcggatgc
      481 aagccaaatt caagtggata agtgtgttga gaatttcgta ttagtttcaa gagacacgct
      541 ttcattattt ctcccgatgc tcaaggagga ggcattaaaa cttcatgtac ataaaaaagt
      601 tccttcatta ctcgttcatc actgtaccag agatattcct gtttttcagg aagttgcgca
      661 actatcgcag aataagaatc ttcgctatgc agaaatgcta cgtaaaaggg cattaatctt
      721 tgcgttgtta tctgtttttc ttgaggatac gcagtttata ccgttacttc tgaacgtgtt
      781 gcaaccgaat atgcgaacac gagtttgtac agtcatcaat aataatatcg cccatgagtg
      841 gacattagcc cgaatcgcta gcgaattgtt gatgagtcca agcctgttaa agaaaaaatt
      901 gcgcgaagaa ggtacgtcat attcacagtt gcttactgag tgtaggatgc agcgtgcttt
      961 acaacttatt gttatatatg gggtttcaat taagcgagtt gcagtatcct gtggatatca
     1021 cagcgtgtcg tatttcattt acgtttttcg aaattattat gggatgacgc ccacagagta
     1081 tcaggagcga tcggcgcagg aattaccgaa ttgtggctcg gcggcaagta tggctgcgca
     1141 agggattttt tacggcactg accgttctgc ggaaggaata agattataga gttttactca
     1201 gacataaaaa aaacccggca taggggaccg ggaagaggat agtctgccgt ctccagacta
     1261 ataaaccgtt ataacactcc ctgttggcac gggaaacttt gtgctctcag taagttaaat
     1321 ataactttta ctggaaataa gatcagccat ttttttataa acataagcta tacgctgtgt
     1381 gaaaatataa tcgtgttgca tttacttatt atcaattaac tgttatgcaa aactactttg
     1441 tggataaaat ttggtcctac caaatatggc agtttttgca cacagaaaca gtctggcgtc
     1501 atttcattag tatactgaca ttgaaataat cgcagtaatg aaatataagg gatagtcatg
     1561 gctcatgtct gctcggtgat cctcgttcgt cgttcattcg atattcatca tgaacagcaa
     1621 aaaatatcgt tgcataacga gagtatcctg ctgctggata aaaatttggc agacgatttt
     1681 gcgttttgtt cactggatac gcgacggctg gatatcgaag agctgacagt ttgccattac
     1741 ttacaaaata ttcgtcagtt gccacgcaat ttaggattgc atagcaaaga tcgtttgtta
     1801 attaaccagt caccccccat acagctggtg acggcgattt ttgatagttt caatgacccc
     1861 cgggtcaatt cgccgatact gagcaaaatg ctctatcttt cctgtttatc aatgttttct
     1921 cataagaaag aactgatccc cttacttttc aatagtatca gtactgtttc aggaaaagtt
     1981 gaacgcctta ttagctttga tatcgctaaa cgttggtatc tacgcgatat cgcagaaaga
     2041 atgtacacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac ctgtttcagt
     2101 aaaatattac tcgcctccag gatgtcgatg gccagacgat tactcgagtt acgtcaaata
     2161 cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcatactt tataaataca
     2221 tttagacaat attatggtgt aacgccacat cagttttcgc aacattcgcc gggtaccttt
     2281 tcctgacata tttcgcattt gaatataggt caggatctca cgtctgcttc atgtgaaact
     2341 ctttcctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt gccaggcggt
     2401 ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct aacaattcat
     2461 acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa tggctggagt
     2521 ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag ctggttgttg
     2581 agtggtgaat ggttagcagg aaagagtaag gctgaaccct cacgttcaac cttactctca
     2641 tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac gaaaaagacc
     2701 ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccattac gccggtacct
     2761 accgcgtttt gcgcaccaga gccggcacca tgactgataa ccagcggcag cacgccgaga
     2821 ataaaggcca gagaagtcat caggattggg cgtaaacgca tacgcgctgc ttcgatgatt
     2881 gcctctaccg gcgttttccc ttctttctgc atcatctcaa cggcaaattc gacaatcagg
     2941 atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa gtaaacgtca
     3001 ttacttaagc cgcgtagatc ggtggccagt aatgcgccaa cgacgcctaa cggaacaacc
     3061 aacatcaccg agaacggaat tgaccagctt tcataaagtg cggcgagggc gaggaacacc
     3121 acgaccagtg aaatggcata tagcgcggga gcctggtttg aggataacgc ttcctgatac
     3181 gacagtccgg tccatgagta gccgacgcct gccggaagtt ttgcgaccag gtctgccata
     3241 aatttcatgg cgtcaccggt acttttcccg gcagccgctt cacctaaaat ctccattgac
     3301 gggatgccgt tgtagcgttc cagtcgcggt gaaccatagg tccattcggt agacgaatag
     3361 gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt gatgttatcc
     3421 ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg cccctggttg
     3481 aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt gatatcagac
     3541 agcgccacac ccatagcctc ggctttcgcg gcgttgacgt tcactttgaa catcggcgta
     3601 tcttccaggc cgttcgggcg taccccgatg acctgattcg gtgattgcgc tgccagtgat
     3661 aacagctcgt ttcgcgcctg ggtcagtttt tcgtgcccca gattgccgtt gtccagcagt
     3721 tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt gaacgggaag
     3781 acgacggctt tattgatact gcttagcgca atcattgccc gctgaataat cgcggtaacc
     3841 gagttttcct cgccgacacg ttcagaccac ggcttgaggc tgataaacgc cagaccgttg
     3901 ttttgcccct gaccactgaa gccaaagcca ccaacggtaa acaccgactg aacattattt
     3961 ttctcttttg tcagataata atccgtcacc tgttgtagca ctttcgtggt gttaaccatg
     4021 gttgcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc ttctggtaag
     4081 aaagaggtcg gcgtacgcag gaacagcacc gccatcccgg cgcaaatcag caggtagacc
     4141 accatgtagc gaccggtaca acgcaacagc gagcgggtgc tgtcggtata gtgctgagtt
     4201 gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg accgccctcc
     4261 ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc tacaaatact
     4321 gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc cccggtcgca
     4381 ccgctcataa aggccatcgg cataaacact gcggaaagaa caacggcaat cccgaccagc
     4441 gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag cttatcttcc
     4501 gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag taaccctatc
     4561 gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgaccgc cgacaagatc
     4621 gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt ggcgcggaaa
     4681 ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac cagcgttttg
     4741 aaaacttcct gaatagaaat tttgataaac ggtgtggtgt cgtaaggata aaccgtcttc
     4801 aggcttgcag ggaaataagc cgataaacgg ttcagctcct ctttcaccgc tcgcgaggta
     4861 tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg tttgccgtta
     4921 tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac atcacgcagc
     4981 agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc ttccggcgtt
     5041 tgcagacgcg tctgcacaat gatcgaggcg ttcagttgct ggtctgccgc ctgtggcatg
     5101 ccacccagtt gaccaccgga aatctggttg ttttgcacct taatctggga aataacatcg
     5161 gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg catggcgtac
     5221 tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg gtctttgata
     5281 ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc agaaataaat
     5341 gccgctacca tcaggatatt actgctcgac ttatcgacgc taattccctg ctgctgcacc
     5401 gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac ctgtgcgata
     5461 tcaggtgatg tccccgtctc gaaggtcaga gtgatggagg cattacctgc cgcatcgctg
     5521 gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat cacctgagtg
     5581 accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgat ggtaatggtc
     5641 ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag accgcctgca
     5701 agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa atagttagcc
     5761 atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc gttttcctgg
     5821 ctggaggaaa ttgcacgtgc tttgataccc ggacgaatgc gttgcaaacc ggaaacgatc
     5881 acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat ggctttgctg
     5941 gcttcgattt cgcgtagctg cacgacatcg tctttatcca gaatgagtgc cgttgcttta
     6001 ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atacattctg gcggctacct
     6061 tcatccacta atgccgtgac atacatgcca ggcagcaagt caccatttgg gttggggaaa
     6121 atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga gaatttcagc
     6181 gtgccggtct ggccgtaacg tttaccattt tccagattga gctgtactgg cgtactgccc
     6241 tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgcaagaa atcctgcacc
     6301 gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag cgaatctgcc
     6361 tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac gcccgtaatc
     6421 ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc cgcttcggcg
     6481 acggtgacat tggcttctgc ttcattcaac tgggtgcgtg cggtgtcgta atcctgacgg
     6541 ctaacatagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg ggcattgctg
     6601 gcggtagaga gcgctttcgc cagcgaacct ttggcggagt tcagctctgc ctgtaaaggc
     6661 gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc gataaagttg
     6721 cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata aggaacggtt
     6781 ctaccgggca attcgctcaa cacattgacc gaaccggggg agagtgtgac gacaccgacc
     6841 tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt gagcatcgcg
     6901 ccgcagaata acaacggtat taacagtttt cttcttctgt tcattttatt ccctgaaaat
     6961 tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg atttacagac
     7021 cagcggcata gtggggttgt cagccagtaa gatgggatgc aagcacagtg atcgacatgg
     7081 tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gtttctgcca gcattcgggc
     7141 aacgaagggt tctactggtg gatacacata ccaggggaat aaagtcgttt tcgtctctcc
     7201 ttcaataatc accgggggta ttgaaatcag catctgcatc cctcgtcatg ccagccatca
     7261 atttcagctg cttatgtcct tactaaaaat aagatgtgat acccagggtg acgatgtcgc
     7321 tcatacgtct taatttcagc ttgtacataa tgttgaattt ctgaatcttt aatgtttgtt
     7381 gcgaaatgtt gagcgttgac gtgatattgc tttcattttt gtttttcaga atcagttgca
     7441 taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc aagtgtttac
     7501 cataagtaat ctcaagaaga tttttgatac tttctttacg gctgacaaaa taaataggcg
     7561 ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc tcggcatcta
     7621 atttctccag gaatttaatc gcttcttcat caaggatatg gttgtgatag gtgtcaatga
     7681 tcaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg atttttatca
     7741 tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct ttcgtcatga
     7801 gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc ttttcattat
     7861 ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac tttgcccttc
     7921 gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc acgcactcca
     7981 tatattgatc ctaagtaaga ttcctggttg ttatcagttt gtaaccagta aacaaaaggt
     8041 taacttttgc ggattactgc ccaagaataa gttatcaata accagtttgt aatctctgaa
     8101 gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt gtttcgactt
     8161 aggctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg attaaataac
     8221 tcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta tgcaatgtaa
     8281 tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca tagcatcaac
     8341 gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa cctgaccacg
     8401 gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggaacg aaaaaaatca
     8461 gattaaattc agtaggttga aataatcaac agcaggcaat tgtttcaatg atagtgcgta
     8521 attgaactga aacactgtat agctaagaga gtattaactg gatcatagtc acttaatgtg
     8581 actatgatcc aggtggaaat agggataatt attgcttttt aacgaacaaa ctggcgaagc
     8641 tgaacagact ggcggcgcta aatatcagtt caattcccac cagtgtggaa accagcgtta
     8701 ccgacaccat cggcgtagca ccaaggaata tccaggcaat gacgatatcc agcacaccaa
     8761 taacgagctg tagccaactg cctttcattg aacgctgacg ataccaactc atcaggcgaa
     8821 taacccctgc cacacagaac aaaccggcaa taaatgccgc aatggcaaaa atgcccagct
     8881 ctggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg aggaaaccgg
     8941 ataataccgg ccagaaatta tgactgcggt tgctgaatag cccgacaata agcgcaatgc
     9001 ccgagcagat taataatgca cccactactg tgcttaaaat gtcgccagag acgaacggaa
     9061 aactgataca cagcaacccg acgataaaca gcagtacggc aataaactgg attgctctgc
     9121 gatgtttttt aagcatctcc agatcaaact tcaaaattgt tgccttatct atatataaca
     9181 tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg ccaaaaacgc
     9241 gtgtaagaat gcagtcgatt taataaaaat ttcctaatta cagtatctga tgcgtctgta
     9301 actcattgta ttgaaataaa aatatctgat tttgatattt tccatcaaca tgacatatac
     9361 agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt tcactttata
     9421 gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct gcttcttctg
     9481 ccagtagtga gcaatgcagc ggatgcacaa aaagcagctg ataacaaaaa accggtcaac
     9541 tcctggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac tgcagttggt
     9601 tttgctgaag cactgaacaa caaagataaa ccagaagatg cggttttaga tgttcagggt
     9661 attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaaa agccagcttt
     9721 aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt ccgggaatgc
     9781 gttacatcgt atttccttgc atattgaaca ggccggaata tcttctttaa aagcagctat
     9841 tcctcctgtt tgtatataat ctctatattg aatgggttac aaaatgaaca tttcatctct
     9901 ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga acgcacaatc
     9961 tgcgctggca gccaatgaat ccgctaaaga tatgacctgc caggaattta ttgatctgaa
    10021 tccaaaagca atgacaccag ttgcgtggtg gatgctgcat gaagaaacag tatataaagg
    10081 tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag tgatcgaata
    10141 ctgcaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat ctaatgactt
    10201 gccgaattaa taaggtacaa gtaaaaagga gtagcaagtt gagccatctt gctgctcctt
    10261 tttgcatttt tatatgacag cagaatttat tatacgactt atacttgcgg taattgtctg
    10321 tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac gcacacatgt
    10381 attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt ttgctgacgt
    10441 gctgtcttta ggtcacgtcg gactggatcc cagccgtatt gctgctcagg ttgtgacggg
    10501 cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg taggtctgac
    10561 gacggcagcg gatatctggg tgaccgccgc cattggtatg gttattggca gcggtatgta
    10621 cgaactgggt atttatggtt cagtgatgac cttgctggtg ctggaagtct tccatcaatt
    10681 aaccttccgc ctgatgaaca aaaattacca tttacagctg acattagtga acggtaatac
    10741 ggtatcaatg ctcgactggt ttaagcagca aaaaataaaa acggatttag tttcgttgca
    10801 ggaaaatgaa gatcatgaag tggtcgccat tgatatccag cttcatgcca cgacgtcgat
    10861 agaagatctg ttgcgattat taaaaggtat ggcaggggtg aaaggcgttt caattagcta
    10921 atctgtatag ttatttattc gcaggagtac cagtgcggta ctcctgttgg ttttaatgat
    10981 cgaggacgat cagtggtgaa tggttggcag gatctttaag tacggtaata tccgcgccgt
    11041 agagcattgt cagcgcctgc tgtgtcaata cgtcctgcgg cttaccattt gcgataacct
    11101 ttcctttttg cattaataca acacggtctg cataacgcgc ggcaagattt agatcgtgca
    11161 atacgcagca aacattgaac tgccgttcgt gaactaactg gcgtaataaa cgaaacaagt
    11221 gctgctgatg gtgaatatcc agcgccgaag ttggttcatc aagaaataac catttcggtg
    11281 agggagtagg ttcccagagt tgcaccagca ggcgcgctaa ttgcacgcgc tgttgttcac
    11341 caccggataa ttgccgatag tcgcggtttg ctaacgcctg acagtcacat aacgccatta
    11401 tttgtgctgt ttcattatcc tgattaccag tacgatgcgg atgccgcccc atctggataa
    11461 cttcttgtac gctgaatgga aacgccatgt ggctattttg acgcatcacg gcccggtgtt
    11521 tcgccagttc agtaatcgac cattcattta atggcttatt aaacaaccga cattcgccgc
    11581 tatcaggttg aagatagccg gttagctggc gaagcagcgt agattttccc gcaccattag
    11641 gcccgaggat ggcaacaatt tccccaccgg gaaaagtcag cgagacatta tcagtcagac
    11701 gccgcccctg aaggctataa accagattct gggcgctgat catgtgatcc tccggttgcg
    11761 cagaatcaac cacataaaat aagggccacc aagcaggctg gttaataatc ccacgggcat
    11821 ttctgctggc tgtaccagcg tgcgggcaag cgtgtctgcc atcagcaata aacaggctcc
    11881 ggcgagggcg gaacaaggga ttagccagcg gtgatttgcc ccggtagtca tgcgaatcag
    11941 atgcgggatc accaggccaa taaagccgat aatgccgctt accgatacgg cagcaccaac
    12001 gagcagtgag ctcactaaca gtaattgctg gcgtttacgc ttcacgttca cgccgaggta
    12061 gtgggcttct tcatcaccga gctgcagtaa attcagcgtt ccggcgagac aagttgttgc
    12121 gataatggcc ggtaggatga atgaactggc aaccaataac gtcggccatt gcgcctgtcc
    12181 aagattgccc atcatccaca atgtgagttg tcgtagctgc tgctcatcgc cgatatagct
    12241 caggatgccg atcgctgcgc cgcacagggc attgatggcg ataccggcga gtaataattg
    12301 catcatgctg ccatcgcgat ggcgctgcgt gatgagaaaa atgatggtgc agaccactaa
    12361 acttccggca atggcaaaca ccatttgctc ataaagcacc agcaccactg ggaaggagaa
    12421 gggcagcaca atagcaacgc caaccattaa tgcagaaccg ctactgacgc caagtaatcc
    12481 agggtcagcc attgggttgc gaaaaagccc ttgcataatt acccctgcgg ttgccaacgc
    12541 tgcacctacc agcaccgcca gcaaaacccg tggtagacga atattgagcc agatatcgcg
    12601 gtattcctca tcaaaaaggg catcaaaact gatctttaac gctccctgac tggcaccaaa
    12661 cagcaccaga gctagcaata atagtgatag ccccataaag accctggcgg aagagagtga
    12721 atccttgagc acgcaaaact ccgttacgac attacggcgc ggacataccg cgccggggga
    12781 tcacgccggt ttcaggtccg gttcaatcag actgtagacc tgctggttaa gtgctggcgc
    12841 atttgccagt tcatggatat gtgcggcgac atcagcacgg ttaacaaaac catgacactc
    12901 ctgattttgt atccgttgtg ctttgcccgt tgccgcacca tcaagcagac cgccggggcg
    12961 gagaatggca taatcaagct ggctggtttg caaccagctt tccgccagcg ttttttcccg
    13021 taccgcctga ccaaacgcag cctttgcccg ctctgataaa aacggccagc tatcgccgca
    13081 tcccaaagag gtcaccagaa tcatgcgact gatgccagcc ttctcggcct catcaatcac
    13141 tgttctgtgc gccagataat cctgagctcc gcccattgtt gagatgataa gcgcatccgg
    13201 gccagctgcg cggcaagcag ccgcgactac gctggcatca caggcgtcgc ctgtaaaaac
    13261 ctgtacgcct tgttgcgcca gtttcgtggc ggcatcggca tgacgaatga cagcaacgac
    13321 cggacgttgt tccgccagcg ccagttcaag cgttctggca cccacacctt ttccgcctgc
    13381 gccaaagagt agccacggtg tcatcaggcg tgttccttta acgatactgc cagagcatgg
    13441 aaggcgctga cttgttcgct caggagctga cgatgatcat ccctaccgag gaagattttc
    13501 agcattgcgc tgccttcctt attgaaaaag agaatagagg cggtgtccat gcccataaat
    13561 ttacgttcga tcaaggcaat atgcgtgcag ttttctgctt tgatatgccc ggacatgccg
    13621 tgctttccgc gtaggttgaa atagccgtgg cgatggaaac ccgaaggcag ttcgccgcta
    13681 aattcgagta ttacatcggt agtatgtact aacgtggtga cgttgcccca ttcgcagacg
    13741 gtatcccaga cagtatcgaa tttatcacct gacacaacag tcggagaggg cagatttttc
    13801 actacctcca gcagcgtggt gttgtattgt tcggcgatcg cctccagcgt accgtctggc
    13861 tccgttttca aaaattcctg taacgagaca tggctcataa atcactcctg ttatggtttt
    13921 tcaagcgcaa ttgctggcgc attaagtacc tgaatcagtt cattaagaga ctgcaaaatg
    13981 ttgcttgccc agaaacgacc ttcattggtc aaccgcagac acgttgacgc atcgcggctt
    14041 aagccttttt ggtgccactg ggccagtaac ggcgcgagtt tttcggcatg tggcgtaagt
    14101 tcgtccagcg gtacacgcgc cgtttcaatg cccgactgca aggtatgacg ccactgcgcg
    14161 ttacgttcgg cgttacgcat gatcatcatc agcggttttt tgcctgccgc gacggattca
    14221 tgccaggtct gaagattgcg ttcgttcatc caggagtacc cattaatcga accgccggct
    14281 ccggaaccaa aggccagaca atcggcacct tgtttaatca gcaggttata gagattgcgt
    14341 tcgcgtgtgg tacggcccca gtggctgtta ctgatgcagc gccaaccggc atcgtccata
    14401 aaatcacatc cttgcaggta aagatcgcga cgttctgccg gagagggcac ggtagtacgc
    14461 ccattttcca cggctttgcc cagcggtgta ttgggcagga cattgagcgc atagagatcg
    14521 acgccgtcga gaccgatatc gcgggcaata gccagatctt ctccccaggt ttgcgcatcc
    14581 tgacccggca gaccaaacag caggtcacag accaccgcgg cacggtcgcg tttgaccagg
    14641 ctttccataa acgcaatagc ggttgggcca tccgaggtgc gggccatttt cttgcggatt
    14701 ttgctgttaa acgactgaat accaattgag aagcggtttg ctccagcatc aaggcaagca
    14761 tcgattcgct cagcgtcaaa attcagtacc cggccttcaa tagtgatttc acaatcaggg
    14821 gccagtggca gcttttctcg taatgtggtg atgattctgg ccaaatcgtg tgctgaaagc
    14881 gcggaaggcg taccgccacc gaaatagacc gcgtggatgg gggcagactg atgcaatacg
    14941 ctgtctgctt ccatttctat ttcacgaatc agggcgtcgg tataatgagc acacgcatcg
    15001 tcattaaaac ggttctgata aaaaccacaa aacgtgcagt gtgtcgcaca aaaaggaatg
    15061 tgtaggtaaa ccagtcgctt acgtggtgat accgtttgat tgatcatctc ttgccaggtc
    15121 tgtgccagtt gctctttggc aaccggaatg gccccacgaa acggcatcat ggcgcgcctg
    15181 tctttaaagg gctgatcacc gtctaaagca aaatgtggcg tgagatccag ggtgttattt
    15241 gtgttcattc gtctgattta aa
//
LOCUS       DAZFFE010000110        15102 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZFFE010000110 DAZFFE010000000
VERSION     DAZFFE010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.110"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(80..775)
                     /gene="aqpZ"
                     /locus_tag="ACHJ69_003843"
     CDS             complement(80..775)
                     /gene="aqpZ"
                     /locus_tag="ACHJ69_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO5016732.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAASFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            1201..2859
                     /gene="ybjD"
                     /locus_tag="ACHJ69_003844"
     CDS             1201..2859
                     /gene="ybjD"
                     /locus_tag="ACHJ69_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO5016733.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNILIGENAWGKSSLLDALTL
                     LLSPESELYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKVISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2856..3812)
                     /gene="ybjX"
                     /locus_tag="ACHJ69_003845"
     CDS             complement(2856..3812)
                     /gene="ybjX"
                     /locus_tag="ACHJ69_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO5016734.1"
                     /translation="MNMSQLTERTFTSSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFCS"
     gene            3963..5078
                     /gene="macA"
                     /locus_tag="ACHJ69_003846"
     CDS             3963..5078
                     /gene="macA"
                     /locus_tag="ACHJ69_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO5016735.1"
                     /translation="MKKRKTVKKRYVIAMVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDNAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKINDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5075..7020
                     /gene="macB"
                     /locus_tag="ACHJ69_003847"
                     /pseudo
     CDS             5075..7020
                     /gene="macB"
                     /locus_tag="ACHJ69_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
     gene            complement(7093..7317)
                     /gene="cspD"
                     /locus_tag="ACHJ69_003848"
     CDS             complement(7093..7317)
                     /gene="cspD"
                     /locus_tag="ACHJ69_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO5016736.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7467..7541
                     /gene="yljB"
                     /locus_tag="ACHJ69_003849"
     CDS             7467..7541
                     /gene="yljB"
                     /locus_tag="ACHJ69_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO5016737.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            7640..7960
                     /gene="clpS"
                     /locus_tag="ACHJ69_003850"
     CDS             7640..7960
                     /gene="clpS"
                     /locus_tag="ACHJ69_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO5016738.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7991..10267
                     /gene="clpA"
                     /locus_tag="ACHJ69_003851"
     CDS             7991..10267
                     /gene="clpA"
                     /locus_tag="ACHJ69_003851"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO5016739.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNSSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     repeat_region   10480..10747
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=10480..10507
                     /rpt_unit_seq="gttcactgccgtacaggcagcttagaaa"
     gene            10920..11903
                     /gene="cas1f"
                     /locus_tag="ACHJ69_003852"
     CDS             10920..11903
                     /gene="cas1f"
                     /locus_tag="ACHJ69_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001729819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated endonuclease Cas1f"
                     /protein_id="HFO5016740.1"
                     /translation="MSSNYLTPSDLKTILHSKRANIYYLEKCRVQVNGGRVEYVTSEG
                     KESYYWNIPIANTTALMLGMGTSVTQAAMREFAHAGVMVGFCGTDGTPLYSANEVDAD
                     VSWLSPQSEYRPTEYLQQWVSFWFVEDKRLAAAKRFQLIRLTHIDKHWPSSKMLREHA
                     FQPDVNALHTLLNRTREEIDAAENHTQLMLVEAKLTKALYKMVSQTVGYGDFTRAKRG
                     GGIDMANRFLDQGNYLAYGLAAVAAWVTGIPHGLAVMHGKTRRGGLVFDLADLIKDAL
                     VMPQAFIAAMAGEDAQEFRQRCINIFQQADALDVMITSLQETAQALAKADQ"
     gene            11900..>15102
                     /gene="cas3f"
                     /locus_tag="ACHJ69_003853"
     CDS             11900..>15102
                     /gene="cas3f"
                     /locus_tag="ACHJ69_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated helicase Cas3f"
                     /protein_id="HFO5016741.1"
                     /translation="MNVLIISRCTKNARVESCRIIDQFAERTGDAAWQTVITLEGVNT
                     LRRLLRKTARRNTAVACHWLKKNGQTELMWIVGNIRRFNAQGRVPTNRTTQISLVNKE
                     ENRWQCAESIALLAAIAGLFHDFGKSGRSFQQSLTQKNTRSYQPYRHEWISLRLFQAF
                     VGDRPDDVWLAKLGELTADDESAILERLQKDTPQFSNSPFSSLQPLAKTVGWLILSHH
                     RMPENLSPQSEQSCKGCESWLEKQLNADWNAVNHRRDDWSQQDFAHVWQFPSGTPLQS
                     ASWREKARQIARRASNALGLKTYGQMEQLFPLHMARFCLMLADHYYSSSDAQQKWQDP
                     DYIVWANSDRQSGTLKQRVDEHLVGVAHHAYLLGRQLMHTRASLPAIARHKGFSQRAT
                     DVNYRWQDKAWDVARALRERSREEGFFGVNMASTGCGKTFANARIMYALADEREGCRF
                     TVALGLRTLTLQTGDALRSRLGLGEDELAVLIGSAAVNQLWQQEKIDNDCGSASQESP
                     AEEQQFVKYEGSLHTGTLEKWLKDDSKLKELVSAPILVSTIDHLISATEGVRGGKQLP
                     AMLRLLTSDLVLDEPDDFDIADLHAVCRLMNWAGMLGTRVLLSSATLPLGLIQALFAA
                     YLAGRKMWQASCGINGRPVNICCAWFDEKDADATQIYDGPGFRDAHAKFVARRAVMLA
                     EKERLHFGRVASVSSASGAIQDVTESVAQTVHTPMLKLHQAHRQRHESGKTVSLGLVR
                     FANINPLVAVTKALIVIPSPEDVCIHYCVYHSRHPLAVRSDIEKRLDRAFTRHNELDF
                     WNNEDIADALHNRPESHHLFVVLGTSVIEVGRDWDADWGIIEPSSMRSIIQFAGRIQR
                     HRRNVPTSENLVILRSNIKSLQKKAPAFCKPGFETKEHLLNSHDICELIPIADYRTIN
                     ALPRILSANNANKLAELEHSRLSAELMSSKSKHVVAAQWWRAPLSWNGWMQRQTPFRY
                     SPLPEAVFFLHMDDEESEARFYSRTGDNERKAQGNFRREEVRCASGVVCWGVMDYQHV
                     LLDLAEKQSCEIASVGEKFAEVRVPVSEEDAVDDWRY"
BASE COUNT         3788 a         3562 c         4150 g         3602 t
ORIGIN      
        1 gattttaacg ccaccaacac ccgtgcggtt tgtaacgtcg aagataaaac caccagggca
       61 gacaacatgc caatgctaat taatcgcgtt tttccagtag agtccggtaa atcagaccac
      121 cgataatacc gccgacaatt ggcaccaccc agaagaacca cagttgttct aatgcccagc
      181 cgccctggaa gatagcaacc gcagtactgc gcgccgggtt aacggaagtg ttagtcaccg
      241 gaatactgat taagtgaatg agggttaagg ccagaccaat ggcaattggc gcaaaaccag
      301 caggagcgaa tttgtcagtt gcgccgtgga tcaccaacag gaaacctgca ctcaatacca
      361 gttcaactac cagcgcggaa agcatggaat aaccgcctgg tgaatgctcg ccataaccgt
      421 tagaagcaaa accgctggct gccgcgtcaa aacccgtttt accactggca attaaataca
      481 gcagcgccgc cgcaacaata ccgccgacaa cctgggcaat tacatagcca acgacttctt
      541 ttgccgggaa acgtccgcca gcccataaac caatagtgac cgccgggtta aaatgaccac
      601 cagaaatatg accaacagca aaggccattg tcagaacggt cagaccgaac gccaacgcca
      661 cgccggcaaa accaatgcct aattccggga agcttgcggc cagtacggca ctaccacagc
      721 cgccaaaaac aagccagaaa gtaccaaaac attcagctgc taattttctg aacatatcca
      781 cctcaattaa aaattgaccc tgtgaaaaat atggtcgttt tatagggtcg tcgtaaaaaa
      841 gtgacgacgg aaataatgcg cggctatttt aaaaacgaag gcaagtcatt caccagataa
      901 ataaatccag taaatttgat ttagggcaac agcgggttgc cccatatagt catttgtctg
      961 attgacagtg tagtgcaagc gaaagattta atcctttagg cgtaataaaa aataatttat
     1021 catgctaatt atttgatttt gttgtttttg cagacttgtc agcaagaggg agtataacgc
     1081 gattattcgc tcatttttca gacatttgcc atgcttaaat gtgatgtcat cacgtattag
     1141 catggccttt cccgttatac tgccagcgta aaggataagt cacatatttc tggaggggat
     1201 atgattcttg agcgcgttga aattgtgggt tttcgcggta tcaaccgttt gtcgttgatg
     1261 ctggaacaaa acaacatcct gattggggag aacgcgtggg gtaaatccag cttgctggac
     1321 gccttaaccc tgctgctatc gccagaatca gaactctacc attttgagcg cgacgatttc
     1381 tggttcccgc cgggagatat caacgggaga gaacatcatc tgcatattat tttgaccttc
     1441 cgcgagtcgc tgccaggccg acatcgggtt cgccgctatc ggccgctgga agcgtgctgg
     1501 acgccatgca ccgatggtta tcaccgtatt ttttatcgtc tggaagggga gagtgcggaa
     1561 gacggcagcg tgatgacact gcgcagtttt ctcgataaag acggacatcc gattgatgtt
     1621 gaggatatta acgatcaggc tcgtcatctg gtgcgtttaa tgccggtgct gcgcttgcgt
     1681 gatgcccgtt ttatgcgccg tattcgtaac ggcacggtgc caaatgtccc caatgtggaa
     1741 gtcaccgcgc gccagctcga tttcctcgcc cgtgaattat cctcacatcc gcaaaatctc
     1801 tctgatgggc agattcgtca gggactttcc gcaatggtac agctgcttga gcattatttt
     1861 tctgagcagg gagctggaca ggcgcgatat cgtttaatgc ggcggcgagc cagcaatgag
     1921 caacgaagct ggcgctatct ggatatcatc aaccggatga ttgaccgacc tggagggcgc
     1981 tcgtatcggg ttattttgct cggcctgttt gctactttat tgcaggcaaa aggcacattg
     2041 cgactggata aagacgcccg tccattgttg ctgatcgaag atccagaaac ccgtttgcac
     2101 cccattatgc tttcagttgc ctggcatctg ttgaaccttc tgccattgca gcgcatcgct
     2161 actaccaact cgggagagtt gctttcgtta acgccggtag agcatgtttg ccgactggta
     2221 cgagagtcct ctcgcgttgc cgcctggcgt ctgggaccga gtggcttgag taccgaagat
     2281 agccgacgca tctcctttca cattcgtttt aaccgtccgt catcgctgtt tgcccgctgt
     2341 tggctgctgg tggaagggga aacggaaacc tgggttatca atgaactggc gcgtcagtgc
     2401 ggacatcatt ttgatgccga agggatcaag gtcattgagt ttgcccagtc cggactaaag
     2461 ccactggtta aatttgcccg ccgaatgggg attgaatggc atgtactggt cgatggcgat
     2521 gaagcaggga agaaatatgc cgctacggta cgcagcctgt tgaataacga tcgggaagcc
     2581 gaacgagaac atttaacggc gttaccggcg ctggatatgg aacattttat gtatcgccag
     2641 ggattttccg atgtgttcca ccgcgtggcg caaatcccgg aaaatgtacc gatgaatctg
     2701 cgcaaagtta tctcgaaagc gatccatcgc tcttccaaac ccgatcttgc cattgaagtg
     2761 gcaatggagg ccggacgtcg tggtgtggac tccgtaccga cgctgctgaa aaaaatgttc
     2821 tcacgcgtgc tgtggctggc gcgcggtcgg gccgactaac tacaaaacat cgtggccatt
     2881 tgtggctgaa tagcgtcgag catctcatag cgccgacggt attcagcccg ttttttactg
     2941 gcgatttcgg caatctcttt tcgtgctatc tgtgctggaa ggcggtaatg gcgttcagca
     3001 tcacatacgc cgccaaccga ttcccagaaa gcgttgtaat cggcatggat cttgccttct
     3061 ttatcgcgat aacgcaggct gcggtaaata tgcgtttcat tgctgacggc aataatctgc
     3121 tctacctgca aacgttgggc aaacagacag gccgcttcca tcacgaggcg tttgggaaat
     3181 agcccgtggc aggctttcgt cgcattctgg atttcctggt gtggaatttc ccattttgcg
     3241 ccttgcagtc cgccaataaa catcgtcctt ttcccctgat attcacacag ggtaaaagtg
     3301 agctctgcca gaggaatacc ttcgctgttg cggaacagga tggtgctgtc accttcttta
     3361 tccattgaga tcatcatggt cagctcaagc gtgaactgct cgccgttttt gccttccagc
     3421 ttcgccagtt gcagcccggg ggtattcaaa tataagctga attcttccgc cgacatacac
     3481 ccgcggagta acgcataatg gtaacgtaac gcctccagca attgcttacg gctaagattc
     3541 gccgcaaggt aagggcgatg tagacgtaca ggcagtcgcg gctggcgcgt taacaacaca
     3601 ttgagattag gccagtggga aagttcgttc atccactcaa cgcttaaacg cggcataatc
     3661 aacgagcgca gcaaaaattt ctggcgaaaa ctacggcgat gccagaattt acccggccga
     3721 cactgtcctc gtgccagact aagaaaaagt gacaggctgc tgagagattc agatgacgta
     3781 aaggtccgtt cagttagctg cgacatattc atgaaatcaa tggttataca tgacgtcgat
     3841 ttaaccattg cgtatcttaa ccaaacatca atagtgtgat tattaacgta aattttaggg
     3901 ttttgttgat atttcgttga agttaatgac ccggattggc atatggagta ttcagaatat
     3961 ttatgaaaaa gcggaaaacc gtgaagaagc gttacgttat tgcgatggtg atagtcatcg
     4021 ccgggctgat tacgttatgg agaattctta acgcacccgt gccgacttat cagacgctga
     4081 ttgtgcgccc cggtgattta cagcaaagcg tgctggcgac cggaaagctg gacgcgctgc
     4141 gtaaggttga cgtgggcgcg caggtcagcg gtcagttgaa aacgctgtcg gtggcgattg
     4201 gcgataaagt aaaaaaagac cagcttttag gggttattga tcctgaacag gctgaaaacc
     4261 agatcaagga ggtggaagca acgctgatgg agctacgcgc gcagcggcag caggcggaag
     4321 cggagctgaa actggcgcgg gtgacgtatt cccgtcagca acgtcttgca caaacgcaag
     4381 cggtttcaca gcaggatctc gataacgccg cgacggagat ggctgtgaaa caggcgcaaa
     4441 ttggcaccat tgacgcgcaa atcaagcgca atcaggcctc tctcgatacg gctaaaacca
     4501 atctcgatta cacccgcatc gttgccccga tggccgggga agtcacgcaa atcaccactc
     4561 tgcaaggcca gacggtgatt gccgcacaac aagcaccgaa cattctgacg ctggcagata
     4621 tgagcaccat gctggtaaaa gcgcaggttt ctgaagcgga tgtaatccac ctgaagccgg
     4681 ggcaaaaagc ctggtttacg gtgcttggcg atccactgac gcgctacgag gggcaaatca
     4741 aggatgtgct accgacgccg gaaaagatta acgacgctat tttctattac gcccgttttg
     4801 aagtacccaa ccccaatggt ttactgcggc tggatatgac cgcgcaagtg catattcagc
     4861 tcaccgatgt gaaaaatgtg ctgacgatcc ctctgtcggc gttaggcgat ccggttggcg
     4921 ataatcgtta taaagtcaaa ttgttgcgta atggtgaaac acgcgagcgt gaagtgacga
     4981 ttggcgcacg taacgatacc gatgttgaga ttgtcaaagg gctggaagcg ggcgatgaag
     5041 tggtgattgg tgaggccaaa ccaggagctg cgcaatgacg cccttgctcg aattaaagga
     5101 tattcgtcgc agctatcctg ccggtgatga gcaggttgag gtgctgaagg gcatcagcct
     5161 cgatatttat gccggtgaga tggtggcgat tgtcggcgct tcgggttccg gtaaatcgac
     5221 cctgatgaat attctcggct gcctggataa ggccaccagc ggcacctatc gcgtcgccgg
     5281 tcaggatgtt gccacgctgg acgccgatgc gctggcgcaa ctgcgccgcg agcatttcgg
     5341 ctttattttc cagcgttacc atttgctttc gcatttaacc gccgagcaaa acgttgaagt
     5401 acccgccgtc tatgccggtc ttgagcggaa acagcggctg cttcgcgccc aggagttact
     5461 gcaacgactg gggctggaag accgtacaga gtattacccg gcacagcttt cgggtggcca
     5521 gcaacagcgc gtcagcatcg cgcgggcatt gatgaacggc ggtcaggtaa ttcttgccga
     5581 tgaaccgacc ggcgcactgg acagccattc tggcgaagag gtgatggcga tcttgcatca
     5641 gctgcgcgat cgcgggcata cggtgattat cgtcacccac gatccgcagg ttgctgctca
     5701 ggccgagcgg gtgatcgaaa ttcgcgacgg cgaaattgtg cacaatcctc ccgccgttga
     5761 aaaagtgaat gctactggcg ggacggaacc tgttgtcaac acggcgtctg gctggcggca
     5821 gtttgtcagc ggttttaacg aggcgctgac gatggcatgg cgggcgctgg cagcgaataa
     5881 aatgcgtact ttactgacca tgctggggat tattatcggt attgcgtcgg tggtttccat
     5941 tgtcgtggtg ggcgacgccg ccaaacaaat ggtgctggcg gatattcgtt ctattggtac
     6001 gaatactatt gatgtctatc ccgggaaaga ttttggcgat gacgatccgc aatatcagca
     6061 ggcgctgaag tacgacgact tatcgctatc caaaaacaac cgtgggttgc ctctgccacg
     6121 cccgccgtct cgcaaaacct gcgcctgcgt tataacaatg ttgatgtggc tgccagtgcc
     6181 aatggcgtga gcggcgatta ttttaatgtc tatggcatga ccttcagtga agggaacacc
     6241 tttaatcagg agcagctgaa cggtcgtgcg caggtcgtgg ttctcgacag taatactcgc
     6301 cgccagcttt tcccccataa agcagatgtg gttggcgagg tgattctggt cggcaatatg
     6361 cccgccagag tcattggtgt ggcggaagaa aaacagtcga tgtttggtag cagtaaagtg
     6421 ctgcgtgtct ggctgccata cagcacgatg tccgggcgag ttatggggca gtcgtggctg
     6481 aactccatta ctgtcagggt gaaagaaggc tttgacagcg ctgaggcgga acagcaactc
     6541 acgcgtttac tttcactgcg ccacggtaag aaggatttct ttacctggaa catggatggc
     6601 gtcttgaaaa ccgttgaaaa gaccacacgt actttacaac tgtttctgac gctggttgcg
     6661 gtgatttcgc tggtggtggg cggtattggt gtaatgaata ttatgctggt gtcagtgacc
     6721 gagcggacgc gggaaattgg cattcgcatg gctgtaggtg cgcgagcaag cgatgttttg
     6781 caacagtttt tgatcgaagc cgtactggtt tgcctggttg gtggcgcgtt gggaataaca
     6841 ctgtcactgt taattgcttt caccttgcag cttttcttac ccggctggga gattggtttt
     6901 tcaccgttag cgctgctgct ggcgtttctc tgctcgacgg tcaccgggat tttatttggc
     6961 tggttacccg cacgaaatgc ggcacgactg gatccagtag atgctctggc acgagagtaa
     7021 tttttgagat aaaaatgcca gccgatcggg ctggcatttt gcctttagga tgtacacaat
     7081 gagacagaag agctatgcga ctgccgcttc tacttcgacg ggcacaataa cactggcgtg
     7141 attgcctttt ggcccctggt ggacatcaaa ctgaacggat tgtccagctt ttagcgttct
     7201 gtaaccatcc atctgaatgg tggaataatg agcgaaaata tcttcgccgc cgccttcagg
     7261 gcagatgaaa ccaaaccctt tggcattgtt gaaccactta acagtaccct tttccatgct
     7321 tcgacatcct tcgcaaatct tatacaagta agatggaata aaccggggtc agagaggggg
     7381 ctgttcaaaa cctcgccaac tctagaaata caatttagag aattagggcg agccgtcaag
     7441 catttgacag gggccagggg gcaggtatga atcaaaaatt tgaagcagtt aacgctattg
     7501 acaggaatgt gacagatgtc gctgatgcca acgatagatg atagttatct atcatgtgga
     7561 gtagattggt caggcaaata agctcttgtc agcggcaggg cgttctgccg ataaccgtaa
     7621 ccgaagatga taactgacaa tgggtaaaac gaacgactgg ctggactttg atcaactggc
     7681 ggaagaaaaa gttcgcgacg cgctaaaacc gccatctatg tataaagtga tattagtcaa
     7741 tgatgattat actccgatgg agtttgttat tgacgtgtta caaaaattct tttcttatga
     7801 tgtagaacgt gcaacgcaat tgatgctcgc tgttcactac caggggaagg ccatttgcgg
     7861 agtctttacc gccgaggttg cagaaaccaa agtggcgatg gtgaacaagt acgcgaggga
     7921 gaatgagcat ccattgctgt gtacgctaga aaaagcctga atgcaggcat aaaaattggg
     7981 ggaggtgcct atgctcaatc aagaactgga actcagttta aatatggctt tcgccagagc
     8041 gcgcgagcac cgtcatgagt ttatgaccgt cgagcacttg ttactggcgc tgctcagtaa
     8101 ctcatctgcc cgggaggcgc tggaagcgtg ctctgtggat ttggttgcgc tccgtcagga
     8161 actggaagcc tttattgaac aaaccacacc cgttctgcct gccagtgaag aggagcgcga
     8221 cacacagccg acgctgagtt ttcagcgcgt actgcaacgt gcggtcttcc atgtccagtc
     8281 ctccggtcgc aatgaggtta ccggtgcaaa cgttctggtc gctatcttta gcgaacagga
     8341 gtcgcaggcg gcatatctgt tgcgcaaaca tgaagtcagc cgtctcgatg tggtgaactt
     8401 tatctctcat ggcacgcgta aagacgagcc gacacagtct tctgatcctg gcagccagcc
     8461 aaacagcgaa gaacaagctg gtggggagga acgtatggag aatttcacga cgaacctgaa
     8521 tcagcttgcg cgagtgggcg gaatcgaccc actgattggt cgtgagaagg agctggagcg
     8581 tgctattcag gttctctgcc gtcgccgtaa aaacaacccg ctgctggtag gggagtctgg
     8641 tgtcggtaaa accgcgattg cggaggggct tgcatggcga attgttcagg gcgatgtgcc
     8701 ggaagtgatg gctgactgta cgatttactc tctcgatatc ggttctctgt tagcgggcac
     8761 aaaatatcgc ggcgactttg aaaaacgttt taaagcgttg ctcaagcagc tggagcaaga
     8821 cactaacagc atcctgttta ttgatgagat ccacaccatt atcggtgcgg gtgcagcgtc
     8881 tggtggccag gtcgatgcgg ctaacctgat caaaccgttg ctctccagcg gtaaaatccg
     8941 cgtaattggt tcgacaacct atcaggagtt cagcaacatt tttgagaaag accgtgctct
     9001 ggcgcgtcgc ttccagaaaa ttgatattac tgaaccgtca atcgaagaaa ctgttcaaat
     9061 catcaatggc ctgaaaccga agtatgaagc gcaccacgac gtgcgttata ccgcaaaagc
     9121 ggtgcgtgcg gcggtagagc tggcggtgaa atacattaac gatcgtcatc tgccggataa
     9181 agcgattgat gttatcgacg aagcgggcgc tcgcgcacgt ctgatgccgg taagcaaacg
     9241 caagaaaacc gttaatgtgg cggatattga atccgtggtg gcccgtattg cacgcattcc
     9301 agagaagagt gtttctcaga gtgaccgcga taccctgaaa aacctcggcg atcgcctgaa
     9361 aatgctggtc ttcggtcagg ataaagccat tgaggcgctg actgaagcca ttaagatggc
     9421 gcgtgcaggt ttaggtcacg aacataaacc ggtcggttcg ttcctgtttg ccggccctac
     9481 tggggtcggg aaaacagagg tgacggtaca gctttcgaaa gcgttgggca ttgagcttct
     9541 gcgctttgat atgtctgagt atatggaacg ccataccgtc agccgtctga ttggtgcgcc
     9601 tccgggatac gttggttttg atcagggcgg tttgctgact gatgcggtca tcaagcatcc
     9661 acatgcggtg ctgctgctgg acgaaatcga gaaagcgcac ccggacgtgt tcaacatcct
     9721 cttgcaggtg atggataacg gtacgctgac cgataacaac ggacgcaaag cggacttccg
     9781 taacgtggtg ctggtgatga ccactaacgc gggggtacgt gaaaccgagc gcaaatccat
     9841 tggtcttatc caccaggata acagcaccga tgcgatggaa gagatcaaga agatctttac
     9901 accggaattc cgtaaccgtc tcgacaacat tatctggttc gatcatctgt caaccgacgt
     9961 gatccatcag gtggtggata aattcatcgt cgagttgcag gttcagttgg atcagaaagg
    10021 tgtttctctg gaagtgagcc aggaagcgcg taactggctg gccgagaaag gttacgaccg
    10081 ggcaatgggc gcacgtccga tggcgcgtgt catccaggac aacctgaaaa aaccgctcgc
    10141 caacgaactg ctgtttggtt cgctggtgga cggcggtcag gtcaccgtcg cgctggataa
    10201 agagaaaaat gagctgactt acggattcca gagtgcacaa aagcacaagg cggaagcagc
    10261 gcattaatct gattgtctgg taggttggtg aagtccgtga tctcgtcagg ggttacggac
    10321 tttttattta tggggggagg aggttcagac ccttttttta atgatgatgc taagttattg
    10381 ataattagtg ctgcgggtag gtaaggataa aaaagggtgg cagcaggaga ttgagatggt
    10441 tttgctttat taacaacggg ctaaacgtgt agtatttgag ttcactgccg tacaggcagc
    10501 ttagaaaagc cggggcgcat ccagccgcac gtaaatgctg ttcactgccg tacaggcagc
    10561 ttagaaatta tggctcgccg cgaagaatac gccgccgcag ttcactgccg tacaggcagc
    10621 ttagaaatgt taacggcgtg gctatgccgg ataatttacg ttcactgccg tacaggcagc
    10681 ttagaaactg aacgttgaag agtgcgaccg tctctccttg ttcactgccg tacaggcagt
    10741 attcacacta cgatagtgtt agacgtttgg tcgtgcaatg acactctcaa cttcaaacca
    10801 ttagcgttag cacgcaataa caatcgtaat aattgcgatg gaaatcaatt ttcagcacat
    10861 aaatcaatgc tgtactaagc ccaatacctt caaatataaa ataatcacag gatgtgttta
    10921 tgtcttcgaa ttaccttacg ccttccgatc tcaaaaccat tctccactcc aaacgcgcca
    10981 atatttatta tctggaaaaa tgccgggtac aggtgaatgg tgggcgggtg gagtatgtta
    11041 ccagcgaagg taaagagtcg tactactgga atatccccat tgcgaatacc acggcgttga
    11101 tgctgggaat gggaacttcc gttacccagg cggcgatgcg tgaatttgct catgccgggg
    11161 tgatggtagg cttttgtggt acggatggca cgccgctgta ttcagcaaat gaagtggatg
    11221 ctgatgtctc ctggctcagc ccacaaagtg aatatcgccc tacagagtat ctccagcagt
    11281 gggtttcttt ctggtttgtt gaagacaaac gacttgctgc cgcgaaacgt tttcagttaa
    11341 tccgcctgac acatatcgat aagcactggc ccagctcgaa aatgctgcgt gaacacgcat
    11401 ttcaacctga tgttaatgcg ctgcataccc tcctgaatcg tactcgtgaa gaaatagacg
    11461 cggcagaaaa ccatacgcag cttatgctcg tagaggccaa attgactaaa gcgttataca
    11521 aaatggtcag tcaaaccgtg gggtatggcg atttcactcg cgcaaaacgg ggcggtggta
    11581 tcgatatggc caaccgcttc ctcgatcaag ggaattatct ggcttatgga ctggcagcag
    11641 tggcggcgtg ggtaacaggt atcccgcacg gtttagctgt aatgcatggt aagacacgtc
    11701 gcggcgggct tgtttttgac cttgccgatc tgattaaaga tgctcttgtt atgccgcaag
    11761 catttattgc cgcaatggcg ggagaagatg ctcaggagtt tcgtcaacgc tgtattaata
    11821 tctttcaaca agcggatgcg ctggatgtca tgatcacctc tctgcaggaa acggcccagg
    11881 cactggctaa ggctgaccaa tgaatgtcct aattatctca cgctgtacga aaaatgctcg
    11941 cgtggaaagc tgccgtatta ttgatcagtt tgccgaacgc actggtgacg cagcatggca
    12001 aacggtgatt actctggaag gcgttaatac actccgccgt ttactgcgca aaaccgccag
    12061 gcgaaatacc gcagtggctt gtcactggct gaagaagaat gggcaaacgg agctgatgtg
    12121 gattgtcggc aatattcgtc gctttaatgc tcaaggccgc gttcccacaa atcgaacaac
    12181 gcaaatcagc ctggtgaaca aagaagaaaa tcgctggcag tgcgcggaaa gtattgcact
    12241 gctggcggct atcgctgggc tatttcatga ttttggtaaa tcaggacgca gctttcagca
    12301 aagcctgact caaaagaata cccgcagtta tcaaccttac cgacatgaat ggatctcact
    12361 gcggctcttt caggcgtttg tcggtgatcg gcccgacgat gtgtggctgg cgaagcttgg
    12421 cgaactcacg gcagatgatg aatctgcgat tcttgagcgc ctgcaaaaag acactcctca
    12481 attttcaaat agcccgttca gttcgctgca accattagcc aaaacggtag gctggctgat
    12541 cctttcccat catcgaatgc cggagaatct ttctccgcag tcggaacaat catgtaaagg
    12601 gtgtgaaagt tggctggaga agcaacttaa tgctgactgg aatgccgtta accaccgcag
    12661 agatgactgg tcacaacagg attttgcgca cgtctggcag tttccatcag gaacgccgct
    12721 tcaaagtgcg agctggcggg aaaaagcgcg tcagatagcc cgccgggcaa gcaacgcgct
    12781 aggtttaaag acatatgggc agatggaaca gttgttcccc ttacatatgg cgcgtttttg
    12841 tctgatgctt gccgatcatt actattcctc aagcgatgcg cagcaaaaat ggcaggaccc
    12901 ggattatatc gtctgggcaa atagcgatcg ccaaagcggc acgctcaaac aacgggtaga
    12961 cgaacacctt gtgggcgtcg cgcatcatgc gtatctgctt gggcggcaac tgatgcacac
    13021 acgggcatcg cttccggcaa ttgcccgaca taaaggtttt agccagcggg ccacagatgt
    13081 gaattatcgc tggcaagata aagcctggga tgtggcgcga gcgttacgtg aacgaagtcg
    13141 tgaagaggga tttttcggcg tcaatatggc ctcgacgggg tgtggtaaaa cctttgcgaa
    13201 cgcccgcatt atgtatgcac ttgccgacga gcgagaaggg tgccgcttta ccgttgcgct
    13261 gggattgcgc acgctgactt tacaaaccgg cgatgctctg cgcagtcggc ttgggctggg
    13321 tgaggatgag ctggcggtac tgattggttc ggcggcagtt aatcaactgt ggcaacagga
    13381 aaaaatagac aatgattgcg gaagtgcttc gcaagagtcg ccggcagagg agcaacagtt
    13441 cgttaagtac gaaggcagcc tgcataccgg gacgctggaa aaatggttga aggatgacag
    13501 caaacttaag gagttagtaa gcgcgccgat tctggtctcg acgattgatc atcttatttc
    13561 cgcgaccgag ggcgtgcgag gcgggaaaca gcttccggcg atgttgcgcc tgttaaccag
    13621 cgatttagtg ctcgacgaac ctgatgactt tgatattgcc gatcttcatg cggtatgtcg
    13681 attgatgaac tgggccggga tgctgggaac gcgggtatta ctttcttccg cgacgttacc
    13741 gctaggtttg attcaggcgc tgttcgcggc ttatcttgct gggcgaaaaa tgtggcaggc
    13801 gtcttgtggt atcaacggca ggcccgtcaa tatatgctgc gcctggtttg atgaaaaaga
    13861 tgctgatgcc acgcaaattt acgacggacc tgggtttcgt gatgctcatg ccaaatttgt
    13921 tgcacgacga gcggtaatgc tggctgaaaa agaacgattg catttcgggc gtgtagcttc
    13981 ggtttcatca gcatcaggcg cgatacaaga tgttaccgaa agtgtggccc agacggtgca
    14041 tacgccaatg ctgaagttgc atcaggcgca tcgccagcga cacgaaagcg gtaaaaccgt
    14101 ttcgctgggc ctggtgcgct tcgcgaatat caatccactg gttgcggtga ccaaagccct
    14161 gattgtcatt ccttcaccag aagatgtctg cattcattac tgtgtttacc actcccgaca
    14221 cccgctggcg gtgcgttcag atattgagaa gcgactggat cgcgctttta ctcgtcataa
    14281 cgaactcgat ttctggaaca acgaagatat cgcagatgcc ttacataacc gtccggaatc
    14341 gcaccatctg tttgtggtgt tggggacatc ggttatagaa gtggggcgtg actgggatgc
    14401 ggactgggga attatcgaac caagctccat gcgttcgata attcaatttg ccggacgtat
    14461 tcagcgccac cgtagaaatg tgccgaccag tgagaatctg gttattttgc gtagcaatat
    14521 caaatcattg cagaaaaaag cccccgcatt ttgtaagcct ggtttcgaaa caaaagaaca
    14581 cttgctgaat agccacgata tttgcgaact gattccgatc gcggactacc ggacgattaa
    14641 cgccttgccg cggatcctgt ctgccaataa tgccaacaaa ctggcggagc tagaacatag
    14701 tcgtttatcc gccgaattga tgagcagtaa aagcaaacat gtggtggcgg cgcaatggtg
    14761 gcgtgcaccg ctaagctgga acggctggat gcagcgccag acgccatttc gttattcccc
    14821 tttgccggag gcggtgtttt ttctgcatat ggatgatgaa gaaagcgaag cgcgtttcta
    14881 ttcccgcacg ggcgacaacg agcgtaaagc gcaagggaat ttcaggcgag aagaggtgag
    14941 atgcgccagt ggcgtcgttt gctggggagt gatggattac caacacgttt tgttggattt
    15001 ggctgaaaag cagagttgtg agatagcaag cgttggggaa aagtttgcgg aagtgagagt
    15061 acccgtcagt gaagaagatg cggtcgatga ctggcgttat ca
//
LOCUS       DAZFFE010000111        15066 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFFE010000111 DAZFFE010000000
VERSION     DAZFFE010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.111"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(30..110)
                     /gene="shoB"
                     /locus_tag="ACHJ69_003854"
     CDS             complement(30..110)
                     /gene="shoB"
                     /locus_tag="ACHJ69_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO5016742.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLLLLFL"
     gene            complement(531..911)
                     /gene="acpS"
                     /locus_tag="ACHJ69_003855"
     CDS             complement(531..911)
                     /gene="acpS"
                     /locus_tag="ACHJ69_003855"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO5016743.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(911..1642)
                     /gene="pdxJ"
                     /locus_tag="ACHJ69_003856"
     CDS             complement(911..1642)
                     /gene="pdxJ"
                     /locus_tag="ACHJ69_003856"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO5016744.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLAEAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFATSLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1654..2382)
                     /gene="recO"
                     /locus_tag="ACHJ69_003857"
     CDS             complement(1654..2382)
                     /gene="recO"
                     /locus_tag="ACHJ69_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO5016745.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2394..3299)
                     /gene="era"
                     /locus_tag="ACHJ69_003858"
     CDS             complement(2394..3299)
                     /gene="era"
                     /locus_tag="ACHJ69_003858"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO5016746.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3296..3976)
                     /gene="rnc"
                     /locus_tag="ACHJ69_003859"
     CDS             complement(3296..3976)
                     /gene="rnc"
                     /locus_tag="ACHJ69_003859"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO5016747.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4248..5222)
                     /gene="lepB"
                     /locus_tag="ACHJ69_003860"
     CDS             complement(4248..5222)
                     /gene="lepB"
                     /locus_tag="ACHJ69_003860"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO5016748.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5238..7037)
                     /gene="lepA"
                     /locus_tag="ACHJ69_003861"
     CDS             complement(5238..7037)
                     /gene="lepA"
                     /locus_tag="ACHJ69_003861"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO5016749.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRGNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7235..7714)
                     /gene="rseC"
                     /locus_tag="ACHJ69_003862"
     CDS             complement(7235..7714)
                     /gene="rseC"
                     /locus_tag="ACHJ69_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO5016750.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVSCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7711..8667)
                     /gene="rseB"
                     /locus_tag="ACHJ69_003863"
     CDS             complement(7711..8667)
                     /gene="rseB"
                     /locus_tag="ACHJ69_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO5016751.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASEALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDIGSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGTAQ"
     gene            complement(8667..9317)
                     /gene="rseA"
                     /locus_tag="ACHJ69_003864"
     CDS             complement(8667..9317)
                     /gene="rseA"
                     /locus_tag="ACHJ69_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO5016752.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9350..9925)
                     /gene="rpoE"
                     /locus_tag="ACHJ69_003865"
     CDS             complement(9350..9925)
                     /gene="rpoE"
                     /locus_tag="ACHJ69_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO5016753.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9922..10077)
                     /gene="rseD"
                     /locus_tag="ACHJ69_003866"
     CDS             complement(9922..10077)
                     /gene="rseD"
                     /locus_tag="ACHJ69_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO5016754.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10333..11955
                     /gene="nadB"
                     /locus_tag="ACHJ69_003867"
     CDS             10333..11955
                     /gene="nadB"
                     /locus_tag="ACHJ69_003867"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO5016755.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGAESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTKGPVPIVPAAHYTCGGVMVDDYGRTDVDGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRCMPYAHDVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(11940..12677)
                     /gene="trmN"
                     /locus_tag="ACHJ69_003868"
     CDS             complement(11940..12677)
                     /gene="trmN"
                     /locus_tag="ACHJ69_003868"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO5016756.1"
                     /translation="MSQSTSVFRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENIYQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12809..14143
                     /gene="srmB"
                     /locus_tag="ACHJ69_003869"
     CDS             12809..14143
                     /gene="srmB"
                     /locus_tag="ACHJ69_003869"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO5016757.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLKDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(14176..15057)
                     /gene="yfiE"
                     /locus_tag="ACHJ69_003870"
     CDS             complement(14176..15057)
                     /gene="yfiE"
                     /locus_tag="ACHJ69_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5016758.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQLLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFTVANELASGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFLPG"
BASE COUNT         3660 a         4044 c         3710 g         3652 t
ORIGIN      
        1 cggacgcacc ttaagtacgt ttccttgtgt tataagaaca aaaggagcag ctgtaaaaca
       61 gcaatgatga ttttgatgac ccgtttaatc aggtatcggc aatcagtcat tcgtttttcc
      121 ttaaacaagg aatgcagccg ttcagattag cccttacatc tccccaaaac tgaacgtgcg
      181 agttattgga atcgagtgct gcactccaca ccagagcttt gacgacacca ctcgtttcaa
      241 tgggggaatt cagtggcatg gtgtaaagca cagcaaaatt gccaataacg aaaccgattt
      301 taatgtactt atcaaaaagc ggtcacgtta atcttttcat caatgtcgat ttcttcgtaa
      361 aggctcgcgt tttttatgtc agagattgca agttgcttgt acaggtcaac gaaattgatc
      421 cataatcatt ttcgttggaa tcgtgtgctg cacaaaatta aagttaaaaa gtaaaacccc
      481 cgttccttac cagttcgggg gttttacttt ataaagagaa cggtattatt ttaactttca
      541 ataattaccg tggcacaagc atagtggcgc tcatcggcaa gcgttacatg catatttaca
      601 acgccgagct tttccgccag ttttaatgcc tcaccccata gccgtagccg tggtttgccg
      661 agctcatcat tgaatacttc aaactgatta aacgccagac cattgcggat ccctgtgcca
      721 aatgcttttg ccgccgcttc tttcacagca aaacgcttcg ccagaaaacg cactggctgg
      781 tggtgcgttt tccagatttc ccattcgtta tcgcttaata cgcggcgtgc caggcgctca
      841 ccggatcggg cgatcaccgc ttctatgcga gcgatctcta caatatccgt acctaaacct
      901 aatattgcca ttagccacgc gcttccagca tcagacgttt catttctgcc accgcatctt
      961 tcagtccggt catcactgca cgaccaataa tggcatgacc gatattcagt tcatgcatct
     1021 cagggatggc ggcaatggct ttcacgttgt gataggtcag accgtgtccg gcgttaactt
     1081 tcagaccgag actagtggca aaggtcgcgg ctttggcgat acgcgccaac tcttgcgcct
     1141 gttcggcgtc agttttggca tcagcatagc aacctgtgtg gatctcgata aacggtgcgc
     1201 caacctctgc cgcagctttg atctgctctt catcggcgtc gataaacaga gaaacctgga
     1261 tcccggcctc cgccagacgt ttacaggcat cgcgcatttt gtcacgctgc cctgcgacgt
     1321 ccaggccgcc ttcggttgtt acttcctgac gcttttccgg taccagacag caaaaatgtg
     1381 gcttcgtctc aacggcgatc gccagcatct cttcggtcac cgccatctcc agattcatgc
     1441 gggtatccag cgtctgacgc aggatgcgca catcgcggtc ggtaatgtgg cggcgatctt
     1501 cacgtaaatg cacggtaatg ccgtctgctc ccgcctgctc ggcaataaac gccgcctgca
     1561 ccggatccgg gtaagcagta ccgcgtgcgt tacgcagcgt tgcaatatgg tcaatgttga
     1621 cgcctaacag taattcagcc atgacaatcc tcatcattca taatgtgttt tcaccgttcg
     1681 cttaggcata aactgccgga acagttccct gctctttaat ggtttaccgc caagatacgg
     1741 cttaagcgcc atgcgggtaa agcgtttcgc ggcgcgcagt gtgtctgcgt caggaaattc
     1801 ccgcgcgttt aacgctttta actgccttcc ggtgaacgtt ttattgtcga taacgacgct
     1861 tgcgataaac cctttttctt cgcgataacg atacgtcatg gtgtcatcta ccggctcgcc
     1921 gctacccgca caatgggtaa aattgacgcc atatcccaga tgcccgagca gtgccagttc
     1981 aaagcggcgc agcgcgggtt ctggcgtacc agtggcacct gcaagtgact gaatgcagtg
     2041 caagtaatcg aaaaagagtt cagagaagcg cgtctcgtat tccagtacgc gggagagaag
     2101 ttcgttgatg tacagaccgc tgtaaagcgt gataccgctt aatggcagcg ccagcgagac
     2161 agcttcagca ctgcgcagtg ttttgacttc accacgccca ccgaagcgaa gcaagagagg
     2221 ggtgaaaggt tgtaatgcac ctttcagagt agagcgttta gagcgtgcgc ctttggcaac
     2281 cagacgcacg cgacccgatt cctccgtgaa gacgtccagc atcaggctgg tttcgctcca
     2341 cgggcgacta tgcaggacaa atgcgcgctg ccagccttcc atcggagtta ctcttaaaga
     2401 tcgtcaacat aaccgagact gcgcagtgcg cgttcgtcgt cggcccaacc agatttcact
     2461 tttacccaca gctcaaggtg aacaggcgct tcaaacattt cctgcatgtc tttacgcgct
     2521 tcaatcccga tggttttgat cttggcccct ttgttgccaa taaccatctt cttctgccct
     2581 tcacgctcaa cgagaatcaa accgttgatg tcataaccgc cgcgttcgtt agagacgaaa
     2641 cgttcgatct ccacggtcac ggagtacggc agttcagcgc cgaggaaacg catcagtttt
     2701 tcgcggatga tttcagacgc cataaaacgc tgtgagcgat cggtgatgta atcttccggg
     2761 aagtgatggg tcgcttcagg cagatgctta cgcacgattg ctgcgatagt gtcgacattc
     2821 agcccggttt cggcagagat cggcacgata tcgaggaagt tcatctggct tgccaggaac
     2881 tgtaagtgtg gcagcagatc ggctttttcc tgcacgttat ccactttgtt caccgcgagg
     2941 attaccggcg ctttgccatc gcgcagtttg ttgagcacca tttcatcgtc cggcgtccag
     3001 cgggtgcctt ctacgacaaa aatcaccagc tcaacgtcgc cgatagagct gctcgccgct
     3061 ttgttcatca ggcggttaat ggcgcgtttt tcttccatat gcaggccagg ggtatcaacg
     3121 tagatcgcct gatacgcgcc ttcagtatgg atccccacaa tgcggtgacg agttgtttgc
     3181 gccttgcggg aggtgatgga gatcttctgc cccagcagtt tgttcaacaa tgtggatttg
     3241 ccaacgttcg gacgtccgac gatagcaata aatccgcagt aacttttatc gatgctcatt
     3301 ccagctccag ttttttcaac gcctgttcgg cggcagcctg ctcagcctta cgacggcttg
     3361 aacctgtgcc aaccaccggt tcactcaggc cgctgacctg gcagtggata gtaaattcct
     3421 gatcgtgtgc ttcgccacgt acctggacga ccagataagt cggcagcggc agatggcgac
     3481 cctgcaaata ttcctgcaga cgcgttttcg gatctttttg tttatcgcct gggctaattt
     3541 cgtccagacg agtttgatac cagttgagga ttaatttctc gacggtttga atatcactgt
     3601 cgaggaatac gccaccaatt aatgcctcga cggtgtcagc gagaattgac tcacgacgaa
     3661 atccaccgct tttaagttca cctggcccta aacgtaagca ctcacctaac tcaaattcgc
     3721 gcgccagttc agccagcgta ttaccacgga ccagcgtggc gcgcatccgg ctcatatcgc
     3781 cttcatcgac acgagggaaa cggtgataaa gcgcattggc gataacgtag ctcagaatag
     3841 agtcacctaa aaattctaaa cgctcgttat gtttactgct ggcactacga tgagttaatg
     3901 cctgctgcaa cagttcctga tgattaaaag tgtagcccag cttccgttga agccgattaa
     3961 ttacgatggg gttcatgcga taccaataaa taaatgcgtc aacaattcag cacacgaaac
     4021 agacctgata tacatggctc ggctaactgc ttcactgcag tttcagttta ccggtatatg
     4081 gggaccaacg ctgtttcgtg tgccgtggca acctggaggt gccaacctta aacttcgggg
     4141 gaatattcta tacacaacga cgggggatgt cgttagccac gggagattta tctcataaat
     4201 aattcacgtt gtcgccataa cggcgacaac gtgaacgaag atggctatta atggataccg
     4261 ccaatgcgac ttaagcgcac accagtcggc cattcgcctt cttgcttatc gaagctcatc
     4321 cagatagccg ttgcccgacc gaccagattc gcttcaggca caaaacccca gtaacggctg
     4381 tccgcgctgt tgtcgcggtt gtcgcccatc atgaagtatt gtcccggcgg aacaatccag
     4441 gttgccagtt gttgccctgg ctgctggtaa tacatcccca cctggtcctg cgcaatcggc
     4501 actgtcagaa tgcggtgcgt cacatcaccc aatgtctctt tacgctcgga aagacgaatt
     4561 ccattttctt tggtttcgtt tttcggcact tcaaagaatc cgctggtcgc ttccccacca
     4621 ttacggcgtg agaaggtctg aacgaaatcg ctcggttcca cgttggagta agtgaccggt
     4681 agcgcatttt cacacgcctg gccggaactg catcccggtt gaatcgtcag ctcttttgag
     4741 accggatcat aagtgacttt atcgcccggc aaacccaccg cgcgcttgat gtaatcaagc
     4801 tttggatctt ccggatattt aaagaccacg atatcgccgc gtttcggatg accggtttcg
     4861 atcagcgttt tctggtagat aggatcttta atgccataag caaacttctc taccagaata
     4921 aaatcaccga ttaacagagt cggcatcatc gaacctgacg ggatctggaa cggttcataa
     4981 ataaacgaac gcacaatcaa tacgatagcc agtaccggaa aaacagaagc accggtttcc
     5041 agccagccag gcttcggcgc aacctttttc agcgttgctt tatccagtga gtcaccggca
     5101 gccgcctgcg ccgctgcctg acgttcccgc cgtttaggtg cgaaaaagaa tttatccacg
     5161 caccataaaa tgcccgtcac cagtgtggca atcaccagaa tcagggcaaa catattcgcc
     5221 atgccaactc ctaagggtta tttgttgtct ttgccgacgt gcagaatggc gaggaacgct
     5281 tcctgcggca gctcgacgtt accgatctgc ttcatgcgtt tcttaccttc tttctgcttc
     5341 tgcagcagct ttttcttacg gctgatatcg ccgccataac atttagccag tacgttttta
     5401 cgcagctgtt tcacggtgga tcgcgcaata atgtgcgtac caatcgctgc ctgaatggcg
     5461 atatcaaact gctggcgtgg gatcagatct ttcatcttct ccaccagctc gcgaccgcgg
     5521 ttttgcgaat taccacggtg ggtgatcagc gccaacgcat caacacgttc accgttgatt
     5581 aatacgtcta cacgtaccat gtcggacgcc tggaagcgtt tgaagttgta atccagagac
     5641 gcataaccac gcgaggtaga tttcaggcga tcgaagaagt cgagcaccac ttccgccatc
     5701 gggatctcgt acgtcagcgc cacctgatta ccgtggtaaa ccatattggt ctgcacgccg
     5761 cgtttttcta cgcacagcgt aataacgttg ccgagatatg cctgcggtag cagcatgtga
     5821 cactctgcaa tcggctcgcg cagttcgtaa atgttattta ccgctggcag cttggatggg
     5881 ctgtcgacgt agataacttc tcttgacgtg gtttcaactt catacactac agtcggcgca
     5941 gtggtgatca gatccagatc gtattcacgt tccagacgtt cctggatgat ctccatgtgc
     6001 agcaggccaa ggaagccgca gcggaaacca aagcccagcg cgcttgagct ttccggctca
     6061 tagaacagtg aggcatcgtt caggctgagt ttacccagcg cgtcacggaa ggcttcatag
     6121 tcgtcggaac ttaccgggaa cagaccagca tatacctgag gtttgacttt cttaaagcca
     6181 ggcagcgcct tttctgccgg attacgcgcc agtgttaagg tatcgcctac tggagcgccg
     6241 tggatatctt taatcgcaca taccagccag cctacttcgc cacatttcag ttcagtacgg
     6301 tcaacctgct tcggcgtgaa gatgcccaga cgatcggcgt tataggtctg cccggtgctc
     6361 atgactttca ctttgtcgcc cttacgcagg gtgccgtttt taatacggat aagtgaaaca
     6421 acgcccagat agttgtcgaa ccaggagtcg ataattagtg cctgcaacgg gccttccgga
     6481 tcgccttccg gcggcggaat gtcgcgcaca agacgttcga gaacgtcctg cacgccaacg
     6541 ccggttttcg ctgaacagcg caccgcgtcg gtggcgtcga tgccgacgat atcttcaatt
     6601 tcttccgcca cgcgttcagg atcggctgcc ggcaggtcaa tcttgttcag taccggaacg
     6661 acttcgagat ccatttccat ggcggtgtag cagtttgcca gggtttgcgc ttcaacgccc
     6721 tgcccggcat cgaccaccag cagtgcgcct tcacaggcag ccagcgaacg ggaaacctca
     6781 taggagaagt ctacgtggcc cggggtgtcg ataaagttaa gctgataggt ttcgccatca
     6841 gacgctttgt agtccagcgt cacgctttgc gctttgatgg taatgccacg ctcacgctca
     6901 agatccatgg aatcgagaac ctgcgcctcc atttcacggt cagacaggcc accgcagatc
     6961 tggataatac ggtcagacag cgtcgattta ccgtggtcaa tgtgagctat gatcgaaaag
     7021 ttacgtatat tcttcataaa gtatgattat tgtgccttaa tgcccggtta accaggcttt
     7081 tagaagtcgc tgttctgagc ttaacgtctg tattaataga aacgccgcat tctacactac
     7141 aacattgagg cgaggaaatg ttcataccgt atggattgtg gtatctggaa acgtcctcgc
     7201 atttgttatg caaaatgcaa caaagccagt gaaatcactg gctcgcgtct tccgaagatg
     7261 tttcaaatcg cacaaggcca ggcggcaatg ccacgcttaa gatgatcggt tgccattctg
     7321 cccgggcagc aaacttacgc gaatagccgc gcgcaatcag gaacccgcca ataccgccga
     7381 ggatcgcacc acataatgct gcgacgtcag aagcaaagag tagctgaaat agcgaagcga
     7441 tgaggaataa tcccaccagc ggcgacatat aaaccagtaa tgcggagcta agcaggctgc
     7501 cttcggcgat ccccaattcc actttttgcc ccggcactaa cggctcatca caggatacga
     7561 caatggtatg cgtggtttgc gggccaagtt tattcaacac acggctaccg caaccggcgc
     7621 gtgaagcgca gctgctgcat gaggctttaa catcacaact gaccagcgcc tgcccgtttt
     7681 cccaggagac gacggtagcc cactctttga tcattgcgct gtcccgaact taatattctc
     7741 ggcaatgcgt ttcgccgttt gcggcggcag ttcaccaaca atggtgattt cggcgttatc
     7801 acgtacgctt gtactgacgg ttctgcgtcc ggtgcgcaac atctgatcgg tgctcgatgg
     7861 cgtagcgcgg ttaacgttta ccgagaagct gaataatccg tcggaataga gacgtgattc
     7921 gataggcatg ttgtccatcg tcggtagcgg acgtcgacta ctggaaactt cgctaaaacc
     7981 ctgtggcagc caggttggcg tccagctgaa tttagctttt tcaccgacag gaacagaaag
     8041 caacggcggc aaatttgcct tcgccagcgt ctgcatactg ctgccgatat cctgattgac
     8101 gttaaaagca atcacgcgaa attgttccag cgtttcacca tcgcgatcaa ggagatcaac
     8161 ccgcatcggt aatttcgatt cggtgtccat ccacacgatg tagctgtagc gtgtaccatc
     8221 tcgggcaacc acgcgaatga cttcgcaaag acgatcagca atacgcgtgc gcccgacgga
     8281 gataaagtcg tagtaaggag aaaggcgttt gaaatcggta tagatgagcg atggcagaga
     8341 atcaacaatg taatcgccat taagcgtgaa cggttcaagt cccggttcaa aatagctgat
     8401 ttcattgccg cgctgtacca cttcccggcg cgggccatcc atttgcaaca attgtgcaag
     8461 aggacggtta tcgaggcgtg catgtcgata acgcagagac tcaacaccct gtttattgat
     8521 gctgatgaat gacagctcgt aattcagtga ctgactggcc aggttcatct gctgtaataa
     8581 cgcctcggac gcgggagtgg ccgaggcgtt agcagagaat aacaggctac ctgtcactaa
     8641 tgacatggca aaccaaagtt gcttcattac tgcgattgcg ttcctaaagt ttgaattcct
     8701 ggcacctgta cagcggcttg ctgagtttgt gcctgctcaa actgaagctg ttcagagtgg
     8761 agtcggcgtt gcagttcgta atcctgcaac attgcattaa tgcgacgacg ctgctcctgt
     8821 acctgctgct gttgaccatt atttgcggtc gcttcagaag gtactcccag actaaccggg
     8881 ctggctttac ccatcatcgg cagtgtatta aatactggcg tttcgggctg ctgggacgtt
     8941 tcagattgcc cattatagtg ctggacgcca acgataactg caagcgatac gcatgctgct
     9001 acgcccattt gggtaagctg tgccgcccac ggacgtactt tctgccagaa tggcattttc
     9061 tgccattgat gcggcgcagg ctgggcttcc gggatcaatg tcgccggttg acgtactggc
     9121 tcttcttcaa tggcggccat cacacgtgaa gagatatcga aatggagcac ctcgggagta
     9181 tcaccccgca ttgagtcacg gatcaagtga tagctttccc aggttttctg catttctggg
     9241 ttatgagcca gttcgttaag cagctcacta tccagcgttt cgccatccat taaagcggaa
     9301 agttgttctt tctgcatgcc taataccctt atccagtatc ccgctatcgt caacgcctga
     9361 taagcggttg aactttgtta tcaatagctt ccctcgctcg gaagatacgt gaacgcaccg
     9421 tacctaccgg acaatccatg atagcggcta tctcttcata gctcaggcca tccagctccc
     9481 gcaaggttat tgccatgcgt aaatcttccg ggagggactc aatagttcgg aaaactatct
     9541 gtctcagttc ttctgacaac attaaattct cagggttcga aatttctttc aacgcgccgc
     9601 cactttcgaa gttttcagct tcaatggcat ccacatcact ggaaggtgga cgacgcccct
     9661 gagcaaccag gtaatttttc gctgtattta cagcaatccg atacagccat gtataaaaag
     9721 cgctatctcc ccggaacgaa tccagcgcac gataggcttt aataaaagct tcttgtacca
     9781 catcgggaac atcacccgac ggcacatagc gggaaaccag actcgccact ttatgctgat
     9841 agcgcactac cagtaagtta aaggctttct gatctccctt ctggacccgt tcaaccagga
     9901 cctggtccgt taactgctcg ctcatccgag gtaaagtctc cccaaaccaa atttccacgc
     9961 gctatcgaaa cgccactcca ttagctgcaa tttgagcaag caaagggtta gagtgtctcg
    10021 tttttgtaaa gttccgtaac gcatctgttt ttgtttgtca tgctgtagac ggatcattat
    10081 ctatcattat aagtctacag aatctgaaca tcgcattatc tgtgtagaaa tgcccattta
    10141 actgcctgaa gagtaaccca acggcctttt tatttcacca cctaatcctc caccagccag
    10201 taacttctcg ttttctcgcc gccctgaggc agcgtgttta gtcactgtaa caaatattaa
    10261 aatagcagat gtttattcgc acaacatgat gttatgctga ccaaaccgtg tttagtaaat
    10321 taaacaaaga aaatgaatac tctccctgaa cattcatgtg acgtgttgat tatcggtagc
    10381 ggcgcagccg gactttcact ggcgctgcgc ctggctgacc agcatcaggt catcgttcta
    10441 agtaaaggcc cggtaacgga aggttcaaca ttttatgccc agggcggtat tgccgccgtg
    10501 tttgatgaaa ctgacagcat tgactcgcat gtggaagaca cattgattgc cggtgctggt
    10561 atttgcgatc gccatgcagt tgaatttgtc gccagtaatg cacgatcctg tgtgcaatgg
    10621 ctaatcgacc agggggtgtt gtttgatacc cacattcaac cgaatggcgc agaaagctac
    10681 catctgaccc gtgaaggtgg acatagtcac cgtcgaattc ttcatgccgc cgacgccacc
    10741 ggtagagaag tagaaaccac gctggtgagc aaggcgctga accatccgaa tattcgcgtg
    10801 ctggagcgca gcaacgcggt tgatctgatt gtttctgaca aaattggcct gccgggcacg
    10861 cgacgggttg ttggcgcgtg ggtatggaac cgaaataaag aaacggtgga aacctgccac
    10921 gcaaaagcag tggtactggc aaccggcggt gcgtcgaagg tttatcagta caccaccaat
    10981 ccggatattt cttctggcga tggcattgct atggcgtggc gcgcaggctg ccgggttgcc
    11041 aatctcgaat ttaatcagtt ccaccctacc gcgctgtatc acccacaggc acgcaatttc
    11101 ctgttaacag aagcactgcg cggcgaaggc gcttatctca agcgcccgga tggcacgcgt
    11161 tttatgcccg attttgatga gcgcggcgaa ctggccccgc gcgatattgt cgcccgcgcc
    11221 attgaccatg aaatgaaacg cctcggcgcg gattgtatgt tcctcgacat cagccataag
    11281 cccgccgatt ttattcgcca gcatttcccg atgatttatg aaaagttatt gggactgggg
    11341 attgatctca cgaaaggccc tgtgccgatt gtgcctgctg cacactatac ctgcggtggt
    11401 gtaatggttg atgactatgg gcgtaccgac gttgatggtc tgtacgccat cggcgaagtg
    11461 agctacacgg gattacacgg cgcgaaccgt atggcctcta actccttgct ggagtgtctg
    11521 gtctatggct ggtcggcggc agaagatatc accagatgta tgccttatgc ccacgacgtc
    11581 agtacgttac cgccgtggga tgaaagccgt gttgagaacc ctgacgaacg ggtggtgatt
    11641 caacataact ggcacgagct acgtctgttt atgtgggatt acgttggcat tgtgcgcaca
    11701 acgaagcgcc tggaacgcgc cctgcggcgg ataaccatgc tccaacaaga aatagacgaa
    11761 tattacgccc atttccgcgt ctcaaataat ttgctggagc tgcgtaatct ggtacaggtt
    11821 gccgagttga ttgttcgctg tgcgatgatg cgcaaggaga gtcgggggtt gcatttcact
    11881 ctggattatc cggaactgct cacccattcc ggtccgtcga tcctttctcc cggcaatcat
    11941 tacataaaca gataaaaagc ctgggtcagc gccgtatacg cttcggaata gttctggtct
    12001 ggcccacgaa tgactaagcg atcgctaaag cattctcccg cctgcgggga gaatgccagc
    12061 agcacccgat gcggcagtcg cgcttcgttt tccgccacat ccgtccgcaa acgtaaatgc
    12121 cagcccatgc ttaatgccag ttcagtaaaa ccattaccaa tctgctctgg cagcactacg
    12181 cagaaaaatc cctcttcggt aatgcactcc gccgcacagg tcagcagcga tgggtgatca
    12241 agcgtagtgg tatagcgagc ctgttcccgt tgaggtgtcg cgcactctac tccctgctga
    12301 tagtaaggtg ggttactgat gattaaatcg aagcgtactg tctgctgtgt gatccactgc
    12361 tgaatatccg ccgtatggac gttaatccgc tctgcccacg gggactggta gatattttcc
    12421 tgcgcctgcg cggcagcttc actttccagt tcaactgcat caatcattac gctgtcatcg
    12481 gttcgctgcg ccagcattaa tgccagcaac ccgctacccg cgccgatatc aaggcaacgt
    12541 tttaccccag ccaccggtgc ccatgcgccc agcaaaatgc catccgttcc cactttcatc
    12601 gcacaacgat cgtgagcaac aaaaaactgt ttaaaagtaa atccattacg acgaaacacg
    12661 gatgtagact gtgacatgaa aataaaacct tgcaggaaaa acggcgatag caccgggtga
    12721 gaacaatacc cgagaagcga tatccataca aacagatgaa gattgcagcc gtaacgtcta
    12781 taatcagcgc cccacacaga ggtagaacat gactgtaacg actttttccg aacttgaact
    12841 cgacgaaagc ctgctggaag ccctccagga taaaggtttc actcgcccga ccgccattca
    12901 ggctgccgcc attccgcctg cgctcgatgg ccgtgatgta ctcggttctg cgccgacagg
    12961 caccggtaaa acggcggcgt atctgctgcc agcgttgcag cacctgctcg atttcccacg
    13021 taagaaatcc ggtccgccgc gtattttgat cctcacccca actcgcgagc tggcgatgca
    13081 ggtagccgat catgcccgcg aactggcgaa acatacgcat ctggatatcg ccaccatcac
    13141 cggcggcgta gcctatatga accacgcgga agtgttcagc gaaaatcagg acatcgtggt
    13201 cgccacgacc ggacgtctgc tgcaatacat aaaagaagag aacttcgatt gccgcgcggt
    13261 tgaaacgctg atcctcgacg aagcagaccg tatgctggat atgggcttcg ctcaggatat
    13321 cgaacatatt gctggcgaaa cgcgctggcg taaacagacc ctgctctttt cggcaacgct
    13381 ggaaggcgat gcgattcagg actttgccga gcgtctgctg aaagatccgg tggaggtttc
    13441 tgccaatccg tccacccgtg agcgcaaaaa aattcatcag tggtattacc gcgccgatga
    13501 cctggaacat aaaaccgcat tgctggtgca cctgttaaaa cagccggaag cgacccgctc
    13561 aattgtgttt gtgcgtaagc gtgagcgtgt gcatgagctg gcaaactggc tgcgcgaagc
    13621 gggcatcaac aactgctatc tcgaaggtga gatggtacaa ggcaagcgta acgaagcgat
    13681 caagcgtttg accgaaggtc gcgtaaacgt actggttgca accgatgttg ccgcgcgcgg
    13741 tatcgacatt cctgacgtca gccacgtctt taacttcgat atgccgcgca gtggcgatac
    13801 ttatttgcac cgtatcggac gtaccgcgcg cgccggtcgt aaaggcaccg caatttcgct
    13861 ggtagaagcc catgaccatc tgctgctggg caaagttggt cgctatattg aagagccaat
    13921 taaagctcgt gtaattgatg agttacgccc gaaaacgcgt gcgccaagcg agaagcagac
    13981 cggcaagcca tcgaagaaag tactggcaaa acgcgccgag aagaaaaaag ctaaagagaa
    14041 agagaagccg cgggtgaaaa aacgccatcg cgacaccaaa aatattggta agcgccgtaa
    14101 accgagcgga acgggcgtgc caccgcaaac gacagaagag taatctcaat gccgggttta
    14161 agcccggcat tatttttatc ccggcaaaaa acactcttca acacactgaa taaaaatgtg
    14221 catcgccggg ctaaccgctt ttccggcatg gtgagcgcac attgccgtaa tggtctgcga
    14281 ctgttcgcca aaaggcaatt cgattaattc gccactcgca agttcatttg ccactgtaaa
    14341 gcgcggcagg taactgaccc cgatatttgc cgcgacacaa cgcttgatgc tttcgatact
    14401 tataagctca atggtgtttt ccaccgtgat ccgtcgctgg cgcagcgtgc tctcaaatat
    14461 ctggcggaag acacattgcg gttcattgat aataaaacta caggcgttat gtcttcccgg
    14521 ctcagtaaaa tcgacatctg caatttgcgg tgaagctacc agcaccagca attgttcacc
    14581 caactctcgt cggttcagag catcatcatt ccctacgcgg taaaagacgc cgacatcagc
    14641 ctcatcattc agtagcgcat cacggatcac gtaacagttc agagactgca acgataaacg
    14701 cacttttggg gcgcgttgcc gaaaacgctg caacacctgc ggcattcggt aagagagcaa
    14761 cgtttcgcct gaaacaacgc gtagttcccc gtccggatcc gactcttttt tcgcagcctc
    14821 gcgtagcgta tccattactc tggtgagttc atagatgtgc ggcagcaact ttttgccttc
    14881 gcgagtgagg cacatccgtc ggccaatttt ctcaaacaat tgtaccgaga actcctgctc
    14941 aagctgctga atatgaaaag tcaccgtcga ttgtgtacag cacaattttt gcgaagctcg
    15001 caaaaaggaa ccctcttcca ccacggtttt aagtgtaata aaacggcgca gatccataac
    15061 cccaaa
//
LOCUS       DAZFFE010000112        14517 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZFFE010000112 DAZFFE010000000
VERSION     DAZFFE010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.112"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..477)
                     /locus_tag="ACHJ69_003871"
     CDS             complement(<1..477)
                     /locus_tag="ACHJ69_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HFO5016759.1"
                     /translation="MMKKLFIATCLLLPGLVYSAATPLPSGFDARMQTVSYNGANTTV
                     IRSKTGFLTSVVFDEGEAVISAKAGFPAGWEITTDDNVVYINPRPVVQEQEGDEGEKL
                     KKVFQPTEKEWDTNLFVRTTKRIYSLDLILLSEEKQAQPAYVVQFRYPSEIAKKNAE"
     gene            complement(474..1160)
                     /locus_tag="ACHJ69_003872"
     CDS             complement(474..1160)
                     /locus_tag="ACHJ69_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virB8 family protein"
                     /protein_id="HFO5016760.1"
                     /translation="MSEKIEKKIETAKSFERQLYAENERSKKNAWRVAVAASLLSLCL
                     GAAICVLVPLKEKELAIVAVGEKTGRTELITQVKQEKILQSEALGRYFVNTYITLREG
                     YNYPSLQYDYETVQLYSSNTVKDDYLRLYNSDMAPDKIYHNNGSYVAVEVISNIISDA
                     TAPDKLASVRFKKTTRNFTTGQVSVSYWTARVTYRFEPEKSVKSSSRELNPLGFTVTS
                     YQTDREARGE"
     gene            complement(1675..2769)
                     /locus_tag="ACHJ69_003873"
     CDS             complement(1675..2769)
                     /locus_tag="ACHJ69_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFO5016761.1"
                     /translation="MAQGFFVKYNSTVMDSVDKISSSYQTQFANDIMSLVTVSVTLYV
                     LWKGYQILASKTQTPLQDLVWDLSKFAIIIMFITNADGYLTAATDALQGMKDGFSGGV
                     SVWQTLDNLWKSTQNLGAEIYSLDKSTYVKDQGVVGQFLIWTGSLILMAVSVVVFLTA
                     DVTMKLLIITAPIFIFCLMFGFIRSMFNNWLQSLFSSILTVLFASLVIRIAMDFQGMI
                     LSHAIRAAQTGNVNLVSTGAMGFMAGFLGALLVLIAKGFAVQLAGAGVEGAVQGAAMM
                     GLGAAGMATGKSLMLGGRAGLGFGLGMAGRTGMNSLSGKAGNLMGRGARTAAEWAGEK
                     GYQALAPTGALGMKARRLASLEKARARNAA"
     gene            complement(2781..3497)
                     /locus_tag="ACHJ69_003874"
     CDS             complement(2781..3497)
                     /locus_tag="ACHJ69_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFO5016762.1"
                     /translation="MKRVKTFMLISLLTASFYSSAGIPVAVDASPEWAVEAARWTERL
                     KQWSETAKHYQGQIQAYKDQLATATGIRNIAAFTNELSNLQSELTNIYKQGNSYISDF
                     TSNPEGALSSQAKNLFSKYGAFDMCNTGYERNDNLCKARIVSTAASIEQGNEINKQLS
                     SAMSQIQSLSSRIEASKDIKESQDLANALQAQSLKMQAIKMQYDVWNNKNKADHEMLL
                     THEQEAFIKQQKEAEPLTFD"
     gene            complement(3509..6256)
                     /locus_tag="ACHJ69_003875"
     CDS             complement(3509..6256)
                     /locus_tag="ACHJ69_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HFO5016763.1"
                     /translation="MSTLYKAMTRPAMYVGVPVVPLTVVAGALFLAGVYISKLIWLAI
                     PVAVFILRMITKQDDHIFNLYFLKLKMLGNSVCNRFFGARAFLSGQYEAVEIDEFVNA
                     MKLNERITTGKYIPYSSHVDKNIVKTKNGDYVATWQLMGINFESISAEMLETIDSQVA
                     TLVRSFSGLPVSFYNHSCRASFYDAFTTKSGNKYADIISDCYYGSMKKNKFKGNTLYF
                     TLIYRPDGRVEKLEKRKKSIKEKKDDIHIHVKRMNEMINTFSGALDKFTCKLLGMYEE
                     NGKVFSSQLSFYNYLLTGKLQKIRVTDSPVYNVLGGVDVFFNHDTGQICRIDGNKFFR
                     SIEIKDFCSETASGVFDVLQYSDADYIITHSYTSMSKSEALSTIKRAEKQLKSTEDDA
                     VTQLQELEKAKNDIVSGDISFGYYHFTIMVMADSIRELDESVSKITADFTDLGIIPAL
                     STMSLPAAYFAQLPAVFHLRPRLSPVSNVNFVELASFHNFYQGKRDKNCWTEAVAILK
                     TPSKQAYYLNLHNSVLFKDETGEKNLANTKVIGTAGSGKTMFLSYLACSLQKYNNPET
                     FADSAKNKKLTCVFLDKDRGAELCIRMLGGEYYTVKSGEPTGWNPFALEATKRNRIFV
                     KQLMEILCTRNGERLSTRERLLISESVDAVMDFPPGEMREYGITRMLEHLMQRDDRDE
                     QENGIILRLSQWANSQAHGWVFDNAKDTFNIQHVNNFGIDGTEFLDDPMVCAPITFYL
                     LYRITQLLDGRRLVIFLDEFWKWLQDEAFSDFVYNKLKTIRKLNGLVIPATQSPDEIL
                     KNKISRAVVEVCSTSIYLANPDADYNDYVEGLKLTPEEFNIIKNLDPMSRQFLIKKSS
                     LKKGDGKSFSALATLDLSGLGGYLKILSASADNLEIFESIYHEGMEPDDWVPEYLERA
                     I"
     gene            complement(6275..6601)
                     /locus_tag="ACHJ69_003876"
     CDS             complement(6275..6601)
                     /locus_tag="ACHJ69_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HFO5016764.1"
                     /translation="MSFKKSVCNCISSSVTAVMQRKNKVVMAVAGATVASPAFADGFS
                     KAETLLQKVKTGLGGLSLVTVTIACLWVGYKVLFGGSTIRECSPIIIGAIVIASAAEV
                     ASMMVN"
     gene            complement(6615..7235)
                     /locus_tag="ACHJ69_003877"
     CDS             complement(6615..7235)
                     /locus_tag="ACHJ69_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO5016765.1"
                     /translation="MASLFPALLLCAASVHPDTINDIARVESGFNPYAVAEIIPERER
                     GSYGKSVISHMPKSKEEALTVIREIENRKRRYSVGLMQITSSNFSFYSTSAEKLLDSC
                     ENLSVFEKIIVDCYKRGRSLENALSCYYTGNFSNGKRKEKEFNNTSYVERIGYTGNEK
                     KYVVPGTRSNGGEQRKNRSHNASVIWPETILKSAFVDNSHPTKVIN"
     gene            complement(7592..7807)
                     /locus_tag="ACHJ69_003878"
     CDS             complement(7592..7807)
                     /locus_tag="ACHJ69_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HFO5016766.1"
                     /translation="MKKYLLFALPFFVVGCSEEVKSVDWWGQHLTEAKQKQAECEKSG
                     SDSQNCKNVKQALFIQSQKDAPVPTFD"
     gene            complement(7804..8097)
                     /locus_tag="ACHJ69_003879"
     CDS             complement(7804..8097)
                     /locus_tag="ACHJ69_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5016767.1"
                     /translation="MTEHDAICISALHQIFSDEEHLSEQQKDIILMYAYGYTLNEIAD
                     FKGLKPSTVRKYLDSVRAELGGVSLAGIRTLVLIRTNALLVSSLSRISERGNL"
     gene            complement(8174..9403)
                     /gene="taxC"
                     /locus_tag="ACHJ69_003880"
     CDS             complement(8174..9403)
                     /gene="taxC"
                     /locus_tag="ACHJ69_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase/nickase TaxC"
                     /protein_id="HFO5016768.1"
                     /translation="MGVYVEQEYRIKRAKGTGRDPKSPKLSGRHIHASKSAFNHKVRH
                     GADKKAYTWTPTKEVTFKITGSGKTAAGIKNGIDYITRNGELEAYCYDGKGSEQTGKG
                     EDFNREFTSTLSKGNDYSRTYRGENIDHVKNMVFSPPPEVGVSREDALKATVEFLKET
                     YPNHAFVAVYHDDKEDHPHVHVNIKLRDEETGRRLRLTKSETRKFRNGFHKKLKGMGY
                     DVTATWKKDPERKREIERLQAENPKRLRNVYKVVDFGETSYQNKAGEKRTPFLTYETL
                     KGGKQVTIWGKDLKNHFESEKLQPGTLIKVKKLAPTLVRSPMFNDDGTVAGYRETHRN
                     NWQIENIALERNRERQVHERETRQPEESDVKKQLSRKHEQGHNIGFALEHGFTKDSEE
                     HKKLRIQQERNWKGLGF"
     gene            complement(9407..9910)
                     /gene="taxA"
                     /locus_tag="ACHJ69_003881"
     CDS             complement(9407..9910)
                     /gene="taxA"
                     /locus_tag="ACHJ69_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxA"
                     /protein_id="HFO5016769.1"
                     /translation="MKMIAFRLSDDELKFAEHNAILSGFTSINAFAKHNVLNIETKPV
                     NIPVNNEPAKLVSVRLYPHEIELVKRNAALHGMSMSREIAIRVRQSLLKNEVCLYPDE
                     VKELKKLSTAVDRVGRNIHFIIKGERFCTVNDPDFRKDVIEVIELCKQIDSKLETLTK
                     SVVNRFG"
     gene            complement(10272..10619)
                     /locus_tag="ACHJ69_003882"
     CDS             complement(10272..10619)
                     /locus_tag="ACHJ69_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO5016770.1"
                     /translation="MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELE
                     QEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPELHRRVAEMAMEEDLSLNAFVNK
                     ALEKEVSNHRAGA"
     gene            complement(10616..10864)
                     /locus_tag="ACHJ69_003883"
     CDS             complement(10616..10864)
                     /locus_tag="ACHJ69_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO5016771.1"
                     /translation="MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRF
                     FNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAGLL"
     gene            complement(11174..11467)
                     /locus_tag="ACHJ69_003884"
     CDS             complement(11174..11467)
                     /locus_tag="ACHJ69_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016772.1"
                     /translation="MLTEAKKMAVEMMFWPACPVDDDYCFDAIMYTSVIDKKFEDEHG
                     VYLHQVYKELCSTPELKKLLKKKAIKLIAFKYDIDLDSANKILMKNMLRRIEN"
     gene            complement(11482..11898)
                     /locus_tag="ACHJ69_003885"
     CDS             complement(11482..11898)
                     /locus_tag="ACHJ69_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5016773.1"
                     /translation="MQGHPCIFLKGVDFMKSVYVVGKGLIVAGNKITIDGKEHVSVEN
                     SENIVVTDDCIKIGNKTVVFKGRSIKIEIHGNVNKIVTENADVSIHGNAGDVETVSGD
                     VDCRQVNGDITTVSGDVTCGDVSGDVTTVSGDINRK"
     gene            complement(11908..12207)
                     /locus_tag="ACHJ69_003886"
     CDS             complement(11908..12207)
                     /locus_tag="ACHJ69_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016774.1"
                     /translation="MKCSSVFTSTTNHVFTFERVTLCTIILMHKDTGQQYVVIFTDNN
                     KIRDYKTGIVPQFGELKQSDVDLVLFYRDEYEKYFDSLKDGDECLSFKDFIECLR"
     gene            complement(12661..12888)
                     /locus_tag="ACHJ69_003887"
     CDS             complement(12661..12888)
                     /locus_tag="ACHJ69_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5016775.1"
                     /translation="MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQ
                     NSQSLSLVEFEFLLLLAGEHPEYVLCKRESK"
     gene            complement(12878..13126)
                     /locus_tag="ACHJ69_003888"
     CDS             complement(12878..13126)
                     /locus_tag="ACHJ69_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016776.1"
                     /translation="MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYD
                     AVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLNET"
     gene            complement(13123..13665)
                     /locus_tag="ACHJ69_003889"
     CDS             complement(13123..13665)
                     /locus_tag="ACHJ69_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016777.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCSSRIKLTSPQFKN
                     YSVHIRMEKDRFSVVGSVDSRSWRSPHHVCTLSRKRNPVDIAADIERKILVNASQEVL
                     QAIEYEKHQVEKKDEILILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGDSYDL
                     QIRGLSIDQLVKITGYLKQL"
     gene            complement(13770..14285)
                     /locus_tag="ACHJ69_003890"
     CDS             complement(13770..14285)
                     /locus_tag="ACHJ69_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO5016778.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELM
                     KAVNAAFDVLMANIDKINQFQSTDEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVW
                     ISGETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRR
                     ATGWQRVETRA"
BASE COUNT         4101 a         3401 c         2519 g         4496 t
ORIGIN      
        1 ttctgcattc tttttcgcta tttctgatgg atagcggaat tgtacaacat aagcaggttg
       61 cgcttgtttt tcttctgaca gaaggattag atccagactg taaatccgct ttgttgttct
      121 gacaaaaaga ttcgtatccc attccttttc tgttggctgg aaaacctttt tcagtttttc
      181 gccttcatcg ccttcctgtt cctgaacaac gggacgcgga ttgatgtaaa caacattatc
      241 atcagttgtg atttcccatc cggcaggaaa accagccttt gcactgataa cagcctcacc
      301 ttcatcgaaa acaactgatg tgagaaatcc tgtctttgac cggataacgg tcgtattcgc
      361 accgttataa ctcacagtct gcatccttgc atcaaatcca gaaggtaacg gagtcgctgc
      421 tgaatacaca agcccaggaa gcaataaaca tgtagcaata aataatttct tcatcattcc
      481 cccctggctt cccgatctgt ctggtaactg gttacagtaa aaccgagagg attaagctca
      541 cgactggaac ttttaaccga cttttctggt tcaaaacgat atgtcacacg agccgtccag
      601 tatgaaactg aaacctgtcc ggtcgtgaag tttctcgtcg tttttttaaa gcgcacagag
      661 gccagtttat ctggcgcggt tgcgtcagaa ataatattgg agatgacctc aaccgcaacg
      721 taagaaccat tattatgata aattttatca ggagccatat cgctgttata cagacgaaga
      781 taatcgtcct ttactgtatt cgaactatat agctgtacag tctcataatc atactgaaga
      841 gacggataat tataaccttc acgtaaggta atatacgtgt ttacaaaata ccgccctagt
      901 gcttcagatt gcaggatctt ttcctgttta acctgagtaa tcagttctgt acggcctgtt
      961 ttttcgccaa cagccacaat agcaagctct ttttctttta atggcacaag aacacaaatt
     1021 gcagccccaa gacataagga cagtaatgat gctgccactg caacacgcca tgcatttttc
     1081 ttagaccgtt cattttctgc atagagttgt ctttcgaagg atttagctgt ctcaatcttc
     1141 ttttcgattt tttcactcat aactgattac ctggctgtgg ttaatgggtt ccagttcgcc
     1201 ggaaacatca gaaggaaggg tattccttgt ggtacatccg gaaatctgaa acaggaaaaa
     1261 caaaagaatc acacatcgca taaattcacc ttattaagcg ttcacgaaca ccttgaagag
     1321 ataaaggcca agatgaagaa aaagaatccc accaataaga agcaaaaagc gaagatccat
     1381 accctttctc catttccccg caagccctaa accaagtctt attcctgcga agcaggtaaa
     1441 cactcctaaa agaaggatta ggacaaaata aagtatcatg acaaaataat gaagcatgtc
     1501 acaaccagct ctgactgatt ccacctaaga ccttaaataa ggtaattccc gtgattaaaa
     1561 taataagctg ggttattagc tttctcttcc tgctccatcc ggaggtgata tgtttatcaa
     1621 caaacgtcag tatccagttc agggacatca cataaaaaaa acgtttcatt atattcatgc
     1681 ggcattcctc gctcgtgctt tttccaggga tgcaagtctt ctcgccttca tccccaaagc
     1741 acctgtcgga gccagcgcct gatatccctt ttcacccgcc cattcagcgg ctgttctcgc
     1801 accacgcccc atcagattcc ctgctttccc tgataaagaa ttcatgcccg ttcttccggc
     1861 catccctaag ccaaaaccta aaccagcacg accgccaagc atcagagact taccagtagc
     1921 catacccgca gcacccagcc ccatcatggc tgcgccctga acagcacctt caacacctgc
     1981 acctgctaac tggacagcaa aacctttagc tataagaacc aataaagccc ccaaaaaacc
     2041 agccataaag cccatagcac ctgtagatac aagatttaca ttccctgttt gtgcagcacg
     2101 aatagcatgg gaaaggatca tcccttgaaa atccattgct atccgaatca ctaaactggc
     2161 aaatagaact gtcagaatac tggagaataa cgattgtaac caattattaa acatgctccg
     2221 aataaaacca aacatgagac aaaagataaa tatcggtgct gtaataatca ggagtttcat
     2281 cgttacatct gctgttaaaa aaacaaccac actaacagcc attaaaataa gcgagccagt
     2341 ccatattaaa aactgtccaa ctacaccttg atctttaaca tatgttgatt tatctaaaga
     2401 gtatatttcc gcaccaagat tttgcgtact tttccataag ttatctaaag tttgccagac
     2461 actcacccct ccagaaaaac catctttcat gccttgcaac gcatcagtag ccgcagttaa
     2521 atacccatcc gcattagtta taaacatgat tatgattgca aattttgata gatcccatac
     2581 taaatcctgc aagggcgtct gagttttact ggcaagtatt tgatatcctt tccatagtac
     2641 atataacgta accgataccg tcactaacga cataatatca ttagcaaatt gtgtttgata
     2701 actggagctg attttatcta ccgaatccat aacggtagaa ttatatttaa cgaaaaaacc
     2761 ctgagccata tatccacctt ttaatcaaaa gttaatggtt cagcttcttt ctgctgttta
     2821 ataaaggcct cttgttcatg tgtgagcaac atctcatgat ctgctttatt tttattattc
     2881 cagacatcat actgcatttt tatcgcctgc atttttaacg actgcgcctg taatgcgtta
     2941 gccaaatcct gggattcttt gatatccttt gaagcctcaa ttcggctact taaactctga
     3001 atctgagaca ttgcacttga taactgctta ttgatctcat ttccctgttc aatactggca
     3061 gccgttgaca caatccgtgc tttacagaga ttatcattac gttcatagcc cgtattacac
     3121 atatcaaagg ctccatactt gctgaacaga tttttagcct ggctgcttaa tgcaccttcg
     3181 ggattactgg taaaatcaga aatataactg ttcccctgct tataaatatt tgtcagttct
     3241 gattgcagat tacttagttc atttgtgaat gccgcaatat tccttatacc tgttgccgtg
     3301 gctaactggt ctttatatgc ctgaatctga ccctgataat gtttcgcagt ttcggaccat
     3361 tgtttgagtc gttctgtcca tcttgcggct tcaacagccc attccggact ggcatctaca
     3421 gcaaccggaa tacctgcgct gctataaaat gaagccgtca ataatgatat cagcataaat
     3481 gtttttacac gtttcataaa ttcacctgtc agattgcccg ttcgagatat tcaggaaccc
     3541 agtcatcagg ctccattcct tcatgataaa tactttcaaa aatttccaga ttatctgcac
     3601 tggcagacaa tattttcaga tatccaccaa gtcccgataa atcgagtgtc gcaagtgctg
     3661 aaaatgattt accatccccc tttttaagac tgcttttttt aatcaggaac tgtctcgaca
     3721 ttggatcaag atttttaata atattaaatt cttctggtgt cagcttaagc ccttccacat
     3781 aatcattgta atcggcatca gggttagcca gataaatact ggtgctgcat acttcaacca
     3841 cagcccgtga aatcttattc ttcagtattt catccggaga ctgagttgcc ggaataacca
     3901 gcccgttcag cttacgaatt gttttcagct tgttatagac aaaatcgctg aatgcttcat
     3961 cctgtagcca tttccagaac tcatccagga aaataacaag acgccgaccg tccagaagct
     4021 gcgtaatgcg atacagcaga tagaaagtaa ttggtgcaca taccatcgga tcatcaagaa
     4081 actcggtgcc atcaatgcca aaattattga catgctgaat attgaatgtg tctttagcat
     4141 tatcaaaaac ccagccatga gcctgactat tagcccattg ggacaaacga aggataatcc
     4201 cgttctcctg ctcatcccga tcatctctct gcataagatg ctcaagcatt ctggtaatgc
     4261 catattcccg catttcgcca ggaggaaaat ccatcacagc atcaacactt tcgctaataa
     4321 gcaatctttc tcttgtcgat aatctttcac catttctggt acacagaatc tccatcagtt
     4381 gcttcacaaa aatacggtta cgttttgttg cctccagagc aaaaggattc catcctgtcg
     4441 gctcaccgct ttttacggtg taatattccc caccaagcat tcggatacat aattcagccc
     4501 ctctgtcctt atcaagaaag acacacgtta acttcttatt ttttgcagaa tcagcaaaag
     4561 tttcggggtt attgtatttc tgtaatgaac aggcaagata tgacagaaac attgtcttac
     4621 ctgaacccgc cgttcctata actttagtat tagcaagatt cttttcacct gtttcatctt
     4681 taaacagtac tgagttatgt aaattcagat aatacgcctg tttgcttggt gttttgagaa
     4741 tagcaaccgc ttctgtccag cagtttttat cgcgtttccc ctggtagaaa ttatggaatg
     4801 atgccagttc gacaaaattt acattcgata ccggagataa acgcggcctt aaatgaaaaa
     4861 ccgccggaag ttgggcaaaa tatgccgctg gcaatgacat ggttgacaat gccggaataa
     4921 tgccaaggtc agtaaagtct gccgtaattt tacttacaga ttcgtcaagc tccctgatac
     4981 tgtccgccat taccattatc gtgaaatggt agtaaccaaa ggatatatca ccggacacaa
     5041 tatcattttt tgccttttcc agttcctgca attgagtgac cgcatcatct tctgtactct
     5101 ttaactgttt ttccgcacgt ttaatggtac tcaatgcttc tgatttgctc atggatgtat
     5161 atgaatgcgt aataatatag tcagcatcag aatactggag cacatcaaaa acaccggatg
     5221 cagtctcact acagaaatct ttaatttcaa tagaacgaaa gaatttattt ccatcaatgc
     5281 ggcatatctg tccggtatca tgattaaaaa atacatccac tccaccaaga acgttataca
     5341 ccggagaatc agtgactctg attttttgca acttccctgt cagaagataa ttatagaatg
     5401 acagttgaga agaaaatacc tttccgttct cctcatacat accaagaagt ttacaggtaa
     5461 atttatcaag agcgccagag aatgtattaa tcatttcatt cattctcttc acatgaatgt
     5521 gaatatcatc tttcttttct ttaatacttt ttttgcgttt ttccagtttt tctacacgcc
     5581 cgtctggtct gtagatcagc gtaaagtaaa gagtgttccc cttgaattta tttttcttca
     5641 tcgaaccata ataacaatct gatattatat cagcatattt attacctgat tttgtggtaa
     5701 aggcatcata aaaactcgcc ctgcatgaat ggttataaaa tgaaacgggc agtccggaaa
     5761 atgaacgaac aagagtggca acctgtgagt caatagtttc taacatctcc gcagagattg
     5821 actcaaagtt aatccccatc agttgccatg tcgctacata atcaccgttc tttgttttga
     5881 caatattttt atcaacatgg cttgaatagg gtatatattt accagtagtg attcgctcat
     5941 tcagtttcat ggcattaaca aactcatcaa tttcaacagc ttcatattgt ccggagagga
     6001 acgctcgtgc gccaaaaaaa cgattacaca ctgagtttcc gagcattttc agcttaagaa
     6061 agtaaagatt aaaaatgtgg tcgtcctgct tagttatcat tctgagaata aaaactgcga
     6121 caggaatagc cagccagatc agcttgctga tataaacccc cgccagaaat aacgcccctg
     6181 caaccacagt aagagggacg acaggaacac ccacatacat agcagggcgt gtcatcgctt
     6241 tataaagtgt agacatacta tcgtccccgc tttattagtt aaccatcatt gatgcaactt
     6301 cagccgcact ggcgataaca attgccccga tgatgatagg tgagcattca cgaatcgtgc
     6361 ttccaccaaa cagaaccttg tagccgaccc acagacaggc aatagtcacc gttaccagtg
     6421 aaaggccacc aagccctgtt ttgacctttt gcagcagtgt ttccgctttt gaaaatccat
     6481 ccgcaaaggc cggtgatgcg actgttgccc cggcgactgc cataactacc ttatttttac
     6541 gctgcattac agcagtgaca gatgaactga tacaattaca tactgatttc ttaaatgaca
     6601 ttttataagt cctgttaatt aataactttt gttggatgtg agttatcaac aaaagcagat
     6661 ttcagaatgg tttcgggcca gataactgac gcattgtgtg aacgattttt tcgctgttct
     6721 cctccgttgc ttcttgtacc tggtacaaca tatttttttt cgttccctgt ataaccaatt
     6781 cgctcaacat aacttgtatt attaaattct ttttcttttc gcttaccgtt actaaaattt
     6841 cctgtatagt aacaactaag cgcattctcc aggctcctcc ctcgtttata acaatccacg
     6901 ataatctttt cgaaaacaga caagttttca cacgaatcaa ggagtttttc tgcggacgtg
     6961 gaataaaaag aaaaattact gctggtaatc tgcatcaatc cgacagaata acgtcttttt
     7021 ctgttttcaa tttctctgat aactgttaga gcttcctcct tacttttggg catatgactg
     7081 ataacacttt tgccatagct accgcgttcc ctttcaggaa taatttcagc cacagcataa
     7141 ggattgaagc cactctcgac ccgtgcaata tcatttatcg tatccggatg aactgacgcc
     7201 gcacacagca acaaggctgg gaataaagac gccatagcta acgctccata tatccatcaa
     7261 tacgcatact ctgcattaat aagtaaataa tttgcttcct cttactaagc aaagatgttt
     7321 tcgttttctc ttcgataaat gcattcaacc tgggatcgag ttttatcgtg acataaaccc
     7381 ctttttccga tgacttatct ggtttctcct tcatttaatc aatccactat aagtgtgttt
     7441 tggatttgtt ttagatgcga acatgattcg caaaataaca attctcaaca atacgatcaa
     7501 ataacattga caaaatggtc atattatgat tcgtgtgatc atgcaaaaaa aaagcgccca
     7561 tatgggcgct ttagtatgat ttaaacatta gttaatcgaa tgtagggact ggagcatcct
     7621 tctgagactg aataaacaat gcttgtttaa cattcttaca gttctgagaa tcagagccag
     7681 acttctcaca ctctgcctgc ttttgtttag cttccgtaag atgctgcccc caccaatcca
     7741 cactttttac ttcttcacta caaccaacaa cgaagaacgg caaagcaaaa agcagatatt
     7801 ttttcataaa ttccccctct cactgatcct ggaaagactt gacacgagaa gggcattagt
     7861 ccgaatgagt accagtgttc tgatccctgc caacgagaca cctcccagtt cagctcttac
     7921 agaatcgaga tattttctga cagttgaagg ctttagtcct ttgaaatcag caatttcatt
     7981 cagggtatag ccataagcgt acatcagaat aatgtctttt tgttgctcac tcaaatgttc
     8041 ttcatcacta aaaatttgat gaagtgcaga aatgcagata gcatcatgct cagtcatgat
     8101 aacctccctc gtaggttgtt gtgatcagca ggtgagcaaa gtagccgctt tgttcacctg
     8161 cgccgaaatc aaattaaaat cccaggcctt tccagttcct ctcttgctgt attcttagtt
     8221 ttttatgttc ttctgaatcc tttgtaaacc cgtgttcaag agcaaacccg atattgtgac
     8281 cttgctcatg ttttctggat agctgctttt taacgtcgct ctcttccggc tgcctggttt
     8341 cacgctcatg aacctgacgt tctctgttcc gctcaagggc aatattttca atctgccagt
     8401 tattgcgatg cgtttccctg tagcctgcaa cagttccatc atcattaaac atcggactac
     8461 gtacaagcgt gggagccagt ttttttactt tgataagcgt tcctggctgg agcttttcag
     8521 actcgaaatg atttttcagg tctttacccc aaatcgtaac ctgttttcca ccttttaaag
     8581 tttcataagt gaggaaaggt gttcgttttt cccctgcctt attctgatat gaagtctccc
     8641 caaaatccac caccttgtaa acattccgga gtcttttcgg attttcagcc tgtaatcgct
     8701 caatctcccg tttacgttcg ggatcttttt tccaggtagc ggtaacatca tatcccatac
     8761 ctttcagttt tttatgaaaa ccgtttctga acttcctggt ttcagattta gtaagtctta
     8821 atcttcttcc tgtttcttca tcacgaagtt taatattaac atgtacatga ggatgatctt
     8881 ctttatcatc atggtatacg gcaacgaaag catggtttgg ataagtctct ttaagaaatt
     8941 ctaccgtagc tttcagagcg tcttccctgc ttactccaac ttctggtggt ggtgaaaaga
     9001 ccatgttttt cacatgatcg atgttttctc cacgatatgt cctggagtaa tcatttcctt
     9061 tagataaagt tgaagtaaac tccctgttaa agtcttctcc tttccctgtt tgctctgaac
     9121 cttttccatc ataacagtaa gcctcaagtt caccattacg ggtaatgtaa tcaataccgt
     9181 ttttgatacc tgcggctgtt ttccctgaac cagtgatttt aaaagtgact tcttttgttg
     9241 gcgtccaggt atatgctttc ttgtctgcgc catgtctgac tttatgatta aaagccgatt
     9301 tactggcatg tatatgtctt cctgataact taggagattt cggatcacgc cctgtccctt
     9361 tagcgcgttt aatacggtat tcctgttcta catacacacc cataaatcac ccaaatcggt
     9421 taacaacact cttggttaac gtttcaagtt ttgaatcaat ttgcttacac agttcaatca
     9481 cttcaataac gtcttttctg aaatcaggat cgttaaccgt acagaatctt tcacctttta
     9541 tgataaaatg aatgttgcga ccaacacgat ctacagcagt tgatagtttt ttaagttcct
     9601 taacttcatc agggtaaaga cagacttcat tctttaacaa agactgacgc acacgaatag
     9661 caatttctct gctcatgctc atgccgtgaa gagcagcatt acgtttaaca agctctattt
     9721 catgagggta aagacgcact gataccagct ttgctggttc attattaact gggatgttaa
     9781 caggcttggt ttcaatatta agcacgttat gcttagcaaa agcattgata ctggtaaatc
     9841 cacttaaaat agcgttatgt tctgcaaatt ttaactcatc atcacttagc ctgaacgcta
     9901 tcatcttcat aagtcacccc aacagagcct gcggcaggca caggcaaaaa catcgttttt
     9961 ggacaatctg attcaaatgt taacatttga catcggattg tctatcctgc attgattgta
    10021 ccatatgtgg cactgtgcct agctataaaa tcgtcgctac atatgtccag atttcgttgc
    10081 tgaaagattc tttcgtcttc gaccatcagg caaacgtata gctgaaagat ttgtgaaaga
    10141 aaaaacaggc ctggcagggc tgggaattgt tacggctgaa gccgtaacgc tttttaagtc
    10201 ctcgctacgc tgcggggctt ccggcgcaaa gcgcctccca tgcccttaca gggcataaaa
    10261 aacaagggca attaagcccc tgcgcgatga ttgctgactt ctttttccag agccttatta
    10321 acaaaggcat tcaacgataa atcttcttcc atcgccattt ctgctacccg acgatgcagt
    10381 tctggatcaa gcctgacgtt aaacacgcct ttaaacgggg tatcaggttc ctttccatcc
    10441 tccacacaag actgtaaata cagatcaaca gatgttttaa actcctgttc cagttcagct
    10501 aatgtagaag cctcataagt taccaggtcc cgaataaacg ccagttttcc atacaggaca
    10561 ttattttcaa aatcgggttc aaccgtgcct aaatatcctt tatattttaa atgtttcata
    10621 ataatccagc ctctttcaga ttctgtttaa ttgctttcag cgtaccacct ttgatataac
    10681 tttccggatg tggacgatgc attaatatgg tgtgattgat ttcagcattg aaaaaccgca
    10741 ctcttgaccc ctgcatttcc tttttgacat atcccaaaga ggaaaacaaa acgaccagtt
    10801 catcccattc aaacgttttt ttactgttta aaaactttgc cagtagctta tctgttttcc
    10861 ccataaatcc atcccctata gatatgcaac taattatagt tacacatggc atttttgtca
    10921 aaaaatacaa aggggcagaa agcccctttt tttatttaaa acatttcata cccttgcaag
    10981 tagtttaacc accaccggaa ttgatcccac tccgtgcacg tcattgccgg aattctggtt
    11041 tcccagcctt tgacaaggga gtcagcaagc tgtacgatct gccgcgttga catttaaaaa
    11101 cctcattcag ttttgattgt cgataacgtg atcaggttgt tccagcaacc tgatcacttt
    11161 cactttcact ttttcagttt tcaatcctcc ttagcatatt tttcattaaa atcttgttag
    11221 ctgaatcaag atcaatatcg tacttaaaag caatcaattt tattgctttc ttttttagaa
    11281 gtttttttaa ttcaggtgtt gagcatagtt ctttgtaaac ctgatgaaga tataccccat
    11341 gttcatcttc aaacttcttg tcaataactg atgtatacat tatagcgtcg aaacaataat
    11401 catcatctac aggacaagca ggccagaaca tcatctctac tgccattttt ttagcttcag
    11461 taagcatatc cgattccgtg atcattttct gtttatatct ccgctgacag tggttacgtc
    11521 accagaaaca tcgccacaag taacatcacc acttacagtg gttatatcgc cattaacctg
    11581 acgacaatca acatcaccac tcacggtttc aacatcccca gcattcccat gaatactgac
    11641 atcagcattt tcggttacta ttttatttac atttccatgt atttctatct taatactcct
    11701 gcctttaaaa accactgttt tattaccaat tttaatacaa tcatcagtga caacaatgtt
    11761 ttcactattt tctaccgaga catgctcttt tccatcgatg gtaattttat taccggcaac
    11821 gattaaacct ttaccaacaa cataaacact tttcataaaa tcaactcctt tcaggaaaat
    11881 gcagggatgc ccctgcatac ataatgctta acgaaggcac tcaataaaat ctttaaaact
    11941 taagcattca tcgccatctt ttaacgaatc aaaatatttt tcatattcgt ccctgtagaa
    12001 aaggactaaa tcaacatcac tctgcttaag ttcgccaaac tgaggaacaa ttccggtttt
    12061 atagtcacga attttattgt tatcagtaaa tatgacaaca tactgttgtc ccgtatcctt
    12121 atgcataagg ataatagtgc aaagcgttac tcgttcgaac gtgaaaacat gattagtggt
    12181 tgaagtaaaa acagatgagc atttcatttt aaatctcctg ccctgctggg ctattgtgag
    12241 aggggccatt cccctttcga tgaaataaca atacgacttt taagtcgtat ttgcaagttt
    12301 tctcccgttt ttttactcga taagtgattg ttatcacgtt ttttaatccg cgtaagcgga
    12361 tggaaggggc gaacgaaaat acctgggcag cacaggagcc acatcgcccc gaagcgcggc
    12421 gcagccgctg acgaacgtac cagggtgcac aggccatccc ttcaccctgc aaagcccaac
    12481 tgaccagcag gccatgatta tgctgatttg cgccgcagaa tgccgcagtt agcgcgagcg
    12541 caagcgagtt taccccgccc tggtgcattg cagcatggcg gggcgctgta ctgcgcgtgt
    12601 gagcgtcagc gagctacggg cgcaactcca ggtactaata taaaaccatc acaggtcatt
    12661 ttattttgac tcccttttgc agagcacgta ttcaggatgt tctcctgcaa gtaacaaaag
    12721 aaactcaaac tcaaccagtg acagactttg actgttttga gtattaactt cctctttttt
    12781 ctgccaggtt cttagcccaa caccataaat atgactgcat tccttttgtg ttaatcctgc
    12841 ttttaaacgt aattttttta cattttcagg tgtattatca ggtttcattt aacataaact
    12901 ccttaattaa atttttaact ggcgtctcat cgtcttccct taatccggca cacgtaaaat
    12961 caatatgttg taattcattc tcattcagta cggcatcata caatgtaatt ttcgtagttt
    13021 tgttcccgat tttaacattt gttctggctt ttatcctttt tgtaagcctg taattgtcac
    13081 cttctttttc aagaaaataa gtcacatcgc ctttattttt tctcataact gtttcaaata
    13141 ccctgtaatt ttaacaagtt gatctatact caagccccga atctgtaaat cataactgtc
    13201 gccctgttcg gtgactttac cgttcaatcc gttttcagct ttaaagcctg tcaatgcccc
    13261 ataccagctt tcaagctgga caagttgcga taacatacct ttcaggatca gaatttcgtc
    13321 ctttttttcc acctggtgtt tctcatactc aatcgcctgt aacacctcct gcgaagcatt
    13381 taccagaatt tttcgctcga tatcggctgc aatatcaacg ggattccgtt ttctggataa
    13441 cgtgcaaaca tgatgcggac tacgccagga gcgactatcc acactcccca ccacagaaaa
    13501 ccggtctttt tccatccgaa tatgcacaga gtaattttta aactgcggtg acgtcagttt
    13561 tatacgagac gaacaatcat caagtttgtt cacgcgccag ccgttcccca ggatacggca
    13621 gacaacttca aagacagggt gatattgttc aaaaaaagag ttcatctgtt tccctttttg
    13681 ctacgtagct gctgttcccg tctttttggg ttcgatgccc caaaagacgg gctggctata
    13741 atatcggggg cttctcgccc ccgttctggt tacgctctgg tttccacgcg ttgccaccct
    13801 gtcgccctgc gctgtccggt tgtaccgtat tttgcgcgga tttcttctat cgtgtgttct
    13861 tcacgattcc agtaactttt atgttcgtct ggacgataaa accattgttt tttctttgcc
    13921 gcccatttac acccgatttc ttttaaagtt tccttatgtg taatggtttc tccactaatc
    13981 cagacccagt tacctatcac ttcaaatacc agaccagata aaccagaaag aacgtttaat
    14041 actttttcca ggtcatcacc gtaattatat cgtgcatgtt catcagtgct ctggaactga
    14101 tttattttat caatatttgc catcaataca tcaaaagctg catttaccgc tttcatcagt
    14161 tcagccccta acgggttacg atctggatga tattttaaag cggcttttct gtatgctgct
    14221 ttgatatctt tttcagttaa ttcgccggat aatccaaaaa cgtttaatgc ttcctggata
    14281 ttcatttttt taatctcctg ccctgctggg ctattgtgag aggggccatt cccctttcga
    14341 tgaaataaca atacgacttt taagtcgtat ttgcaagttt tcttccgttt ttttactcga
    14401 taagtgattg ttatcacgtt ttttaatccg cgtaagcgga tggaaggggc gaacgaaaat
    14461 acctgggtag cacaggagcc acatcgcccc gaagcgcggc gcagccgctg acgaacg
//
LOCUS       DAZFFE010000113        14270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFFE010000113 DAZFFE010000000
VERSION     DAZFFE010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.113"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(117..785)
                     /gene="artM"
                     /locus_tag="ACHJ69_003891"
     CDS             complement(117..785)
                     /gene="artM"
                     /locus_tag="ACHJ69_003891"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO5016779.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(785..1501)
                     /gene="artQ"
                     /locus_tag="ACHJ69_003892"
     CDS             complement(785..1501)
                     /gene="artQ"
                     /locus_tag="ACHJ69_003892"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO5016780.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1508..2239)
                     /gene="artI"
                     /locus_tag="ACHJ69_003893"
     CDS             complement(1508..2239)
                     /gene="artI"
                     /locus_tag="ACHJ69_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO5016781.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2257..2985)
                     /gene="artP"
                     /locus_tag="ACHJ69_003894"
     CDS             complement(2257..2985)
                     /gene="artP"
                     /locus_tag="ACHJ69_003894"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO5016782.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3203..3718)
                     /gene="ybjP"
                     /locus_tag="ACHJ69_003895"
     CDS             complement(3203..3718)
                     /gene="ybjP"
                     /locus_tag="ACHJ69_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5016783.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3844..4167
                     /gene="ybjQ"
                     /locus_tag="ACHJ69_003896"
     CDS             3844..4167
                     /gene="ybjQ"
                     /locus_tag="ACHJ69_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO5016784.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4164..4994
                     /gene="amiD"
                     /locus_tag="ACHJ69_003897"
     CDS             4164..4994
                     /gene="amiD"
                     /locus_tag="ACHJ69_003897"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO5016785.1"
                     /translation="MRRVFWLVAAALLLAGCTGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVETASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(4991..6004)
                     /gene="ybjS"
                     /locus_tag="ACHJ69_003898"
     CDS             complement(4991..6004)
                     /gene="ybjS"
                     /locus_tag="ACHJ69_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO5016786.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPSLTHYGVSKLNFDFTLDITRAQEELGYQPVLTLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6103..7533)
                     /gene="ybjT"
                     /locus_tag="ACHJ69_003899"
     CDS             complement(6103..7533)
                     /gene="ybjT"
                     /locus_tag="ACHJ69_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO5016787.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLLPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPQKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKRIARLAEQSTD"
     gene            complement(7544..8545)
                     /gene="ltaE"
                     /locus_tag="ACHJ69_003900"
     CDS             complement(7544..8545)
                     /gene="ltaE"
                     /locus_tag="ACHJ69_003900"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO5016788.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGLYALKNNVARLQEDHDNAAWMAKQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSRAQLAEVAAHWRAFLAR"
     gene            complement(8582..10300)
                     /gene="poxB"
                     /locus_tag="ACHJ69_003901"
     CDS             complement(8582..10300)
                     /gene="poxB"
                     /locus_tag="ACHJ69_003901"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO5016789.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIVAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGIKGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(10433..11401)
                     /gene="hcr"
                     /locus_tag="ACHJ69_003902"
     CDS             complement(10433..11401)
                     /gene="hcr"
                     /locus_tag="ACHJ69_003902"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO5016790.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSRWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVISGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(11413..13065)
                     /gene="hcp"
                     /locus_tag="ACHJ69_003903"
                     /gene_synonym="priS"
     CDS             complement(11413..13065)
                     /gene="hcp"
                     /locus_tag="ACHJ69_003903"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO5016791.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(13209..14108)
                     /gene="lysO"
                     /locus_tag="ACHJ69_003904"
     CDS             complement(13209..14108)
                     /gene="lysO"
                     /locus_tag="ACHJ69_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO5016792.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHHQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         3469 a         3801 c         3752 g         3248 t
ORIGIN      
        1 tgaaataaaa aaagacggac agcttagtgg gttgtccgtc ttcataataa gaatttatgc
       61 actatgtagg ccggataagg cgtccacgcc gcatccggca tcagcatgca gctacattaa
      121 ttccgccgtt cgaatgccag tgctttacgc tcgatcagac gcatcatcag cgtcagcagg
      181 ccgttgacga ccaggtaaat aatccccgcc gcgccaaata ccattacatc gtaggtgcgt
      241 ccgtacaaca actggctgta tcccatcact tccatgagcg taatggtgta agccagagag
      301 gtacttttga acaccagcac cacttcgttg gaataagaag agagcgagcg tttaaacgcg
      361 tacggtagca ggatcgccag cgtatctttt ttgctcattc ccagggcgct acaggactgc
      421 cattgacctt ccgggatcgc gcgaatcgca ccgtaaaaca gctgcgtggt gtacgccgca
      481 ctgttcagcg acaacgcaat cagcgcacat aaccacggtt ctgacaacaa atgccacagt
      541 gccggatact cctgcaacgt cgggaactgg cccggcccgt aataaatcag gaagatctgc
      601 accagcagcg gcgtaccggt aaacagcgtg atatagcccc gtaccagcca caccagcacc
      661 ggcgttttca gcgtcaggat gatggtaaaa atcaatgcca gaatcagtgc cacaatcagc
      721 gaggcaacgg ttagcgtcag gctggtgtgc agccctttca tcagttcggg taaatactca
      781 aacattagct gggcctccgc tcaaaacgtg tcgcgcgcag gtcaatgcgt ttgagaatgt
      841 actgactgag cagggtgatc accaggtaaa tcgccgccgc cacaatgtac caggtaaacg
      901 gttcctgtgt acgggtagcg atgcttttgg tttgtagcat taaatcattc acactaatca
      961 aactgaccag cgcggtatct ttcagcagca ccagccactg gttaccgaga cccggcagcg
     1021 catgacgcca catctgcggc atcaccagac ggaaaaagat agccgatttc gacagcccca
     1081 gcgcctgacc ggattcccac tgacccaccg gcaccgcttt taatgcacca cgcagcgttt
     1141 gcgaggcata ggcggcatac agcagtgaca gagcgatgac accacaaagg aacgggctaa
     1201 cgtcgaagtt ctcaatgtcc atctgcaccg ggatctgcac gaacccaaga ttgatagtga
     1261 agccatccga aagcgtcagc agcagctgcg aggagccaaa atagataaac agcaccacca
     1321 gaatttctgg caggccacgc agaatggtta ccagcgctga acctgcccac gcgacaggac
     1381 gccatttcgc agactcccat accgcaaaga acatcgccag cgccagcccg acaatcaatg
     1441 cacaaacggc aaggccgacg gtcatcccgg cggcgcttgc taaaggaaaa aattcattca
     1501 tcaggaatta cttctggaac catttgttgt agatggtttc gtaagtgcca tctttcttca
     1561 ctttttccag cgcagtgttg agtttctgct gcagttcagt gttgccctgg cgtactgcga
     1621 tgccgaggcc agtaccgaag taatctttat cggtcacttt atcgcccact gccgccagtt
     1681 tcgggttatc tttcagccat tcggttacca ctgcggtgtc accaaatacg ctgtcgatac
     1741 gaccgttttg cagatccaat tttgcgttct ggtaactgtc atatggcacg gtagtgattt
     1801 ccggatgctt atccataatg aacttctggt gtgtcgtccc gttctgaacg ccgacttttt
     1861 tgcctttcag ctgatcaaca ctggtgtatt tgccttgctg acccacaaac agggcagagt
     1921 tgtcatagta cggggtggta aacagcacct gcttttcacg ctccggagtg atatccatgc
     1981 ccgccatcac tgcttctacg cgacggaatt tcaggcttgg gatcaggctg tcaaacgcct
     2041 ggttagagaa agtgcaggtt gcatcaatct ctttacacaa tgcctgcgcc aggtcgacat
     2101 caaaaccaac gatctggttg tttgcatcaa tcgattcaaa cggaggatag gaggcttcgg
     2161 tggcaaaacg aatggtttcg gctgctgtgg cggaaagact aaaacctgca attaacgcgg
     2221 caatcagaac ttttttcatt gttgttatcc cgaatcttag tgagagagat agtttttaaa
     2281 tgcttcggtt tgcggctcgg taaagcagct cgcgtcgcct tgttctacga tatgaccgtt
     2341 ttccatatac accactcggc tggcggtttt acgcgccact tcaacttcgt gggtgacgat
     2401 cacctgggta atattcgttt ctgccagctc acgaatgatg ctgacgattt gtgccgtaat
     2461 ttcggggtcc agtgcggcgg tcggttcatc gaacagcagt acctgcggtt ccatcatcaa
     2521 cgcacgggca atagcaacac gctgctgctg acctccagaa agatgcagcg ggtaacgatc
     2581 gctataaggt ttgagacgca gacgttccag cagtttttct gcacgggcca gcgcctgatc
     2641 tttactcaac cccagtacac gacagggcgc ttcaatcagg ttttgctgca cggtcagatg
     2701 cggccacagg ttgtattgct gaaacaccat gccaacgtta cgacgcaaat cgcgaatcgc
     2761 tttgtcagag ggggttttgg tgaaatcgaa atggttgcct gcaatgttga gcgtaccgga
     2821 gcgcggcatc tcaagcagat tgagtacacg cagcagcgag cttttacccg cgccgctggg
     2881 gccaagtaac accagcgttt cgccctgtgg gcaatccagc gtgatatcga acagcgcctg
     2941 atgcgcgccg tagaagcaat taatgccgtt taattgaata ctcattgaca ctcgtatact
     3001 ggcagtctga tagctattga ggtcgaagat agtacctttg acagaataat tatgcaatat
     3061 ttctgcttta aaagttaaaa gcaaagcgca ttattcaata aacatagcac aaaataacgg
     3121 gggcggtggt cggcgagcat aaatgtcggc attcctcacg aaatgccgga caatttaagg
     3181 ggtttattgg ttgatcaaga cgttagcgat tctcgatgga ctgacggagc gtacccgctg
     3241 tggcatgaac gctaccgcct aagtagcgca catcgtcgat gacccaacac tggccttcct
     3301 gaatcattaa cacttcatcc tgccagccct ggtcaccctg cttgagatcc acgcgcaatg
     3361 gaatgttacg ggcatcacga ttagggatag tcgatgcact ggctacgtgg gcgctgtctg
     3421 gcaaggtggt tcgactggag aacggatcgc tgcttaacaa ttcacgatga ctattatcgc
     3481 ggctggcatc gctaagtagg gttgccagtt tgtcactcaa ataggggcgc agagcggtaa
     3541 tgtcattgct gcgatgttga atgcggtagt catagaactg ttgagccacg ctatcaggcc
     3601 ccccctggac acaggaaccg gtgcgggggc cgttatcttt ataagctggc gtgacggtgg
     3661 tacaggcact gagtagcagt gcgcaaggga taagcatggt caatttgcta tagcgcatga
     3721 tgatttcctt ataagcgaac accttgaaag cgttctacga taataatgat atcctttcaa
     3781 taatagcgta tcagtccgat aatgctttga agatcgaagg cttagcaaac aaggagatcg
     3841 atcatgcaat tttctacaac cccaactctg gaaggccaga ccatcgttga atattgcggt
     3901 gtggtgactg gcgaagcgat tttaggcgcg aatattttcc gtgatttctt tgccggtatc
     3961 cgcgatatcg ttggcggacg ctccggtgcc tacgaaaaag aactgcgtaa agcgcgggag
     4021 attgcctttg aggagctcgg ctcccaggcg cgagcgctgg gggctgatgc cgtcgtcggt
     4081 attgatatcg actacgaaac ggtcgggcaa aacggcagta tgctgatggt tagcgtcagc
     4141 ggtacggcgg tgaaaacgcg tcgatgagaa gagtcttctg gctggtcgct gccgctctgt
     4201 tactggcagg gtgcacaggc gaaaaaggca ttgtcgagaa agagggatat cagcttgata
     4261 cccgacgcca ggcgcaggcg gcgtatccgc gcattaaagt gctggtgatc cactacaccg
     4321 cagatgattt tgatagctcg ctggcgacac tgaccgataa gcaggtcagc tcgcattatc
     4381 tggtccctgc ggtgccaccg cgatacaacg gtaaaccgcg catctggcaa ctggtgccgg
     4441 aacaggaact ggcctggcat gcggggatta gcgcctggcg cggggcaacg cgccttaacg
     4501 acacctctat tggcattgag ctggaaaacc gtggctggca gaaatcagcc ggagtgaaat
     4561 attttgcccc gtttgaaccg gcacagattc aggcgcttat tccgctggcg aaagatatta
     4621 ttgcccgtta tcacatcaag ccggaaaacg tagtagcaca tgcggatatt gcgccgcagc
     4681 gcaaagacga tccggggcca ttattcccct ggcagcaact ggcacagcag gggattggtg
     4741 catggccgga tgcgcagcgg gtgaactttt accttgccgg gcgcgcgcca cacacaccgg
     4801 tagagaccgc gtcattgctg gagcttttgg cgcgttatgg ttatgacgta aagcctgata
     4861 tgactccgcg cgagcagcgg cgcgtgatta tggcgttcca gatgcatttc cgcccgacgt
     4921 tatataacgg cgaagcggat gcagaaactc aggcgattgc cgaagcgttg ctggagaaat
     4981 acgggcagga ttagcgcggc agttttccgt ggtcgcgcag ccaggcggca gttttctcga
     5041 taccttcatc cagggtaagg actggctgat aacctagctc ttcctgcgcg cgcgtaatat
     5101 ccagcgtaaa gtcaaaattc aacttggaga cgccgtagtg ggttagcgac ggctctttag
     5161 ctgacttgcg acctaaacgc tccatgctgc gggcgatcat atccagcatt gggtagggga
     5221 cggaacgaat acgacagtca atattcaact cgtcgatcag cttctgcacg atgctgcgca
     5281 gtgtgcgatg ttcgccgttg gtgatgttgt acacacgccc ggaaggtagc ttatcgcacg
     5341 cttcctggct tgccagccac atggcgtgca cggcattttc atagtaggtc atatcgacca
     5401 gcgcactgcc gccatgcggt aacagaatac tgccgtagtg atgcatcata tgcgccagac
     5461 gggggataaa gactttatcg tgcggtccga acagactttg tgggcgcaga atagtaaagc
     5521 gtgtttgtgg attcgcctgc gaaagcatat tgatcacttc ttcactggct gctttgctgc
     5581 gggcaaactc gttggcgaag cggtgagggc gaaaatcttc tttaatatcg cgatggtggt
     5641 gataatcgaa gtacagggag ggagaagaga tatgaataaa gttacgcaca ccccaggcga
     5701 cagcccattc acccaggcgg cgagtggcgc gaacgttagc cagatcgaaa gcctgttgtg
     5761 tcccccaggg cgaggtaaag ctggagcagt gccacagcgt atcaatgcct gcgagcatca
     5821 ctttagcttg tgatgagacc agctcggtca gatccgccgg aacaaactct gcgcccattt
     5881 tttccagcaa tttacccatt gcctcgttgc gaccggtcgc tcgcacgctg atgcctttct
     5941 ggcataaaaa ctctaccgcg tttcgaccta agccgctggt ggcgccggta accagtacct
     6001 tcatatcaat ccactgttgt tgagaaaata acgtgcgcat tcttccgtga tttcctccat
     6061 gatgcaatgg gaaacatgaa agaataacgc aggttttgtc gattaatctg tgctttgttc
     6121 tgccagtctg gcgattcgtt ttgccattcc gcgaaaaata aacagatgcg cggggatcat
     6181 caataaccag taaaacaacc ctggcatacc gtgcggatgc cagaatgcgc gaacatcgat
     6241 agtacgatag tcacctttat cttccagggt aaaacacagt cgtcccagcc ccggcgcttt
     6301 catgccaaat aacagcgtaa gttgtttttg cggttcaacg acaatcactt tccagctatc
     6361 caccgcatct ccggtctgca aatattcacg ctccgggcgg cctttcgcca gcttatgacc
     6421 gatcgcgcgg tccatcaacg cccgggtttg ccacaaaata ttgccaaaga aataacgctc
     6481 tttaccgccg atttgattca ccacctgcca taaagccgca aggctggcgg acgttttaac
     6541 ggtaaacccc gcctgtttgg cgaaataacc gtactccggt cgccagcggg caaaggcctg
     6601 agcgtcgtag ccccagtcgc tggagttgac cagcttttct tcctctttca acgtgctacg
     6661 taccgcgtca tcgaaagcga tcagccgttg tgggatgagc gcacgtagcg cggtgtcatc
     6721 cgccagcaga tcgtgtttca gcccctgaat caacgccctg gcggtggtgg gcggtactga
     6781 agtaatcaca ttaagaaacc acaccgaaat ccagcgggtg gggaggggga tggggagcaa
     6841 ccagcggcgc ttaccgctca ccgccataaa atgttcaaac tgttgctgat aactgagcac
     6901 ttctggtccg gcggcttcga agatgcgatg ttcgctggct ggatggtcta acagcgccac
     6961 cagatagtgc agcaagtttt ccagcgcgat gggcgtggtg cgtgaacgaa cccagcgtgg
     7021 cggcgttaac actggcaggt tgtagaccat atcgcgcatg acttcgaacg ccgctgaacc
     7081 tgcaccaacg attattccgg cacgcaattc ggtcaccggg acacccgctt cacgaagaat
     7141 atccgccgta gcctgacggg cgcgcagatg gtccgactgc tcatgtggcg gggcctgcaa
     7201 cgaactgaga aagattaatt gctttaccgg tacttcgcgc agcgcatcgc ggacgttgag
     7261 agccacctgg cgctcctgag cgataaaatc accgccttct cccatgctgt gcaccagaaa
     7321 atagaccgta tcgatatcct gcaacagggc cggaaggtta tccggccagc tgagatcgac
     7381 tttatggcaa ctgacatttg ccagttgcag ctttgcaagc ctgtcgacat gacgtgccgc
     7441 cgccaggatc tgatgcccct gctggctgag tgttcgcacc agatgctgac caatgtagcc
     7501 actggcaccg agaactaaaa tgcgttgcgg cacgtctccc tccttaacgc gccaggaatg
     7561 cgcgccagtg ggcggcgact tccgccagct gagcgcgcga gacgtcaaga tgcgtcacca
     7621 ggcggacaat cggcgaggcg ttaatcagta cgtttctcgc tttcatgtat tcgcctaacg
     7681 cggcagcatt ttcttccccg acgcgaacaa acagcatatt ggtgtcctga cgcatcacat
     7741 ccgcgcctgc ttcacgcagt tgcttcgcca tccaggcggc gttgtcgtgg tcttcctgca
     7801 aacgtgcgac gttatttttc agcgcataca gcccggcggc agccagaatg ccggactggc
     7861 gcatcccgcc acctgtcatt ttccgccagc gaatggcacg tttaatgtaa tcacgattac
     7921 cgacgagtaa tgaaccgact ggcgtcccaa gacctttcga caggcaaatg gtgaacgagt
     7981 cacaatattg tgtgatctct ttcagttcgc agccgtaagc caccacggca ttaaagatgc
     8041 gcgcaccgtc aacatgcagc gccagattgc gcttgcgggt aaattcccat gcctctttta
     8101 agtattcacg cggcagcact ttgccgttgt gggtgttttc cagactgagt aatttggtgc
     8161 gggcgaaatg gatatcgtcg ggtttgattt tcatcgccac tttatccagc ggtagcgtgc
     8221 cgtcggcagc tgcgtcgatg ggttgcggct gaatactgcc cagcactgct gcgccaccgg
     8281 cttcaaacag atagttatgc gcggcctgac cgacaatata ctcttcgccg cgttcgcagt
     8341 gactgagcag agcgaccagg ttagcctggg tgccggtcgg cagaaaaatg gcggcttctt
     8401 taccggaaag ctctgccgca tagtcctgca gagcattaac ggtagggtcg tctccgtaaa
     8461 cgtcgtcccc aaccggggcg gccatcattg cttcgagcat ggcgcggctc ggtcgggtaa
     8521 cggtatcact gcgtaaatca atcatggcat gtccttatta tgacgggaaa tgccaccctt
     8581 tttaccttag ccagttcgtt ttcgccagtt cgatcacttc atcaccgcgc ccgctgatga
     8641 ttgcgcgcag catatacagg ctgaaacctt tggcctgttc gagtttgatc tgcggtggaa
     8701 tggctaattc ttctttggcg acaaccacat ccaccaacac cggaccgtcg atggagaagg
     8761 cgcgttgcag agcttcatcg atttcagacg ctttttctac acgaataccc ttaatgccgc
     8821 aggcttcggc aattcgggca aagttggtgt cgtgtaattc ggtgccgtca gtcaaatagc
     8881 caccagcttt catctccatc gccacaaagc ccagcacgct gttattaaag acgataattt
     8941 tcactggcag cttcatctgc actaccgaga ggaaatcgcc catcaacatg ctaaaaccgc
     9001 catcgccgca catggcgacc acctggcgtt ctggctcggt tgcctgcgcg cccagcgcct
     9061 gcggcatggc gttagccatc gaaccatggt taaacgaacc taacaggcga cgcttgccgt
     9121 tcatttttag ataacgtgcc gcccacaccg ttggcgtacc aacgtcacag gtaaagatgg
     9181 cgtcatcggc ggcaaaatga ctaatttgct gcgccagata ttgcgggtga atggctttct
     9241 cgctcggttt agctaaatca tccagccctt tgcgggcgtc gcggtaatct tccagcgctt
     9301 tatccagaaa cttgcgatcg gttttttctt ccaccagtgg cagcaatgcc cgcagggttg
     9361 atttgatatc gccgaccagc gccatatcta ccttgctatg cgcgccgatg ctggctgggt
     9421 tgatatcaat ctgaataatt ttggcatcgg tcgggtagaa cgcgcggtag ggaaactgag
     9481 taccgagcag cactaacgta tcggcgttca tcatggtatg gaaacctgac gaaaagccga
     9541 ttaatcccgt cattccaaca tcatatggat tatcgtattc gacatgttct ttaccgcgca
     9601 gggcatgaac tataggcgct ttaattttcc cggcaaactc aactaactct ttatgcgccc
     9661 ccgcacagcc gctgccacac atcagggcga tattgctgga ataacgcagc agttgcgcca
     9721 gtttgcgtaa ctcttcttct tccggtgtta ctaccggctg tggcgcatga taccagtggg
     9781 tagttgcccc ttctggcgca ggttttaacg ccacatcgcc cggtaacaca acaacggaaa
     9841 cgccacggtt aagcaccgct ttgcgcatag caattgccag cacttgtggg atctgctctg
     9901 ggctggaaac cagctcgcaa tagtgactac attcgcggaa tagctcttgt gggtgggttt
     9961 cctggaaata gccgctgcca atttcgctgg agggaatatg agcgacaatc gccagtaccg
    10021 gaacgtgatt gcggtggcaa tcgaacaggc cgttgattaa gtgcaggttg ccgggaccgc
    10081 acgatccggc acagaccgcc agttctccgc taagttgtgc ttcagcgcca gcggcaaacg
    10141 ccgccacttc ttcatgccgg gtggacatcc actcgatggt gcccatgcga ttaagactgt
    10201 cactaagacc gttcagagag tcgcccgtga ctccccagat gcgtttcacg cctgccgatt
    10261 cgagtgtttt ggcgatataa gctgcaaccg tttgtttcat ggttctccat ctcctgaatg
    10321 tgatgacggt aacaagttta gttcatctga cggaggggga agggatggga gaaaaaggag
    10381 gcactaacgg ttaaatagcc cgatgaaagg aatatcatcg ggcatcaggc gattatgcga
    10441 gaaccaaatc cccctgcgga tggcaggagc aggccagtac gtaaccttca gcgatttcgg
    10501 cgtcggtcag cgtcattgtg ctgctcaccg tatattcacc ggaaatcact ttcgtcttac
    10561 agcagccgca aacacccgca cgacaggcgg cgacaaccgg aacgttattg ctttccagcg
    10621 cctccagtag cgtggtgcca accggagcgt aaaattctcg tgccggttgc agtttggtga
    10681 atttcagacc gctggtcgcc gcttccgcta ctggggtgaa gaatttctct ttaaagaaac
    10741 gcgtcacgcc gagcgctttc acttcctgct ctacccaatc catatatggc gcagggccac
    10801 aggtcatcac ggtacgtgag gctaaatcag ggacacctgc cagcagttcg cgagtgagac
    10861 gaccagcgat aaagccttcg gtaacgttat tttccgccac cagcgttacc ggatagttac
    10921 gccactcatc ggcgaaaatc acatcctgcg gcgtacgcac gttgtagatc acctgcacat
    10981 cggcctgtgg acggttcttc gccagccagc gacgcatcga cataatcggc gtaacgccgc
    11041 agcctgccgc cagcaacagg aatttatctt ctgctttatc gtcgcaggta aattccccca
    11101 tcgcgtccga aagccagaga taatcaccgc gttttacatc gcgcgtcagc caccgggagc
    11161 cgacaccgtc atcaatccgc cgcacggtca gggtgatgta ttcactcacg cctggcgtgg
    11221 aggaaatggt gtaagcacgc agcgtttccg ctgagttacg cacgctgacc agcgcatatt
    11281 gcccggcgcg atacgggtag taatcgtggc aaatcaggga aatcgtccac acatccggcg
    11341 tttcttgcgt aatgtgatga acctgcatcc gccacgggca ttgattcgtt ggcatcgtca
    11401 tcgacaaact ccttacgcgc tcaacagttg cttcatgtct tcttcaacag tggtgataga
    11461 acgcaggccg aatttctcgt tcagcaccgc cagcaggtct ggtgtcagga aaccaggtgc
    11521 agtcgggccg gtgacgatat ttttcacacc cagagaaagc agcgtcagca gaatgacaat
    11581 cgctttctgt tcaaaccagg agagcaccag cgacagcggc agatcgttga ctccgcagcc
    11641 cagtttctct gccagcgtta ccgccagaat aattgctgag taagcatcgt tacattgacc
    11701 agcatctacc agacgcggca gaccttcgat atcgccaaac tcaagtttat taaagcgata
    11761 tttaccgcaa gcgagggtca ggatcaggca gtcatccggc acgctggtgg cgaaatcggt
    11821 gaaatagtga cgctcgccgc gtgcgccgtc acagccgcca agcaggaaga tatgacgcag
    11881 tttttcacgg ctcaccagat caatcagcgt atcggctgcg ccaagcagtg tctggcgacc
    11941 aaagcccacg gtgatcaggt gcggaatttc gctgtacggg aagcccgcca tctgttgcgc
    12001 ctgggcaatg actgcagaga aatcttcgcc atccagatga cgcacacccg gccagccaac
    12061 gatgctgcgg gtccagatac gatcgtcata agcgcctacg gttgggtcga tgatgcagtt
    12121 cgaggtcatc acgatggggc ctgggaaacg agcgaactcc acttgctgat tctgccagcc
    12181 gctgccgtag ttaccgacca gatgcttgaa tttacgcagc tccgggtaac catgcgcagg
    12241 cagcatttcg ccgtgggtgt agacattaac gcccgtgcct tcggtctgtt ccagcaggtt
    12301 gtagagatct ttgagatcgt gaccggaaat cagaatgcat ttacccgccg tcgctttgac
    12361 attgacctgg gttggggtcg ggtgaccgta tttaccggtt tcgcctgcat ccagaatgct
    12421 catcactttg aaattcatct ggccgatttc cattgaacac tcaagaagcg cgttcatatc
    12481 ggcaggccag gtccccagcc acgccatgat tttatggtac tgggcataaa tatcgttgtc
    12541 gtattgaccg agaacatgcg cgtgttccat ataggccgcc gcacctttca ggccatacag
    12601 gcacagcaga cgcaggccga gaatgttttc gccaatcgcc gctttatctt tgttaggggt
    12661 aaattctgcc gcctgacgtt gcagctcgcc gagatcgtcg ctcaccagtt gcaggtcagc
    12721 catcgggtta tcgacgcggg cgttggcatc tacagccagg cattgtgctt tcagcgcctc
    12781 gcgcagggca atcgcttcac gagcgtagcc gacaatacgc ggagaatcga agttaacgtt
    12841 ggtcagggtt gagaaaaagg cacgtggcgc aaagctgtct acatcgtggt tgatgatgcc
    12901 gtattcacgt gctttcactg cccaggccga aagaccttgc agcgccgcga tgagtaaatc
    12961 ctgaaggtca gaggtttccg ccgttttacc acacatcccc tgcgcgtatg agcagccgtt
    13021 tcctgccgga gtacggatag tttgttcaca ttgcacacaa aacatgatca caccttttaa
    13081 agttatattt aatatacatg tttaaggtta agacgcttaa cgcggggata aaagagattt
    13141 ttaatgcaac tttaagggag attgatttag cgcaattttg gcggcagaga tctaccgcca
    13201 gagaggtatt atgcagagaa aaaggcgatg agaatcggca ctaacagact aagaataaaa
    13261 ccgtgaacaa tcgccgccgg gaccatatcc agcccgcctg tacgttgaag aacgggcagg
    13321 gtgaaatcca tcgacgtcgc accgcataag cccagggcag tagagcggct acggcgaatc
    13381 agcccaggga ttaacataat agcaatcagc tcacgggcca gatcattaaa aaacgccgcg
    13441 ctcccgatta ccggaccaaa agattcggtc aataaaatac cggaaagaga ataccagccg
    13501 aaaccggagg ccattgccag tgcggtattg atgggaagat caagaataaa ggcgttaatt
    13561 aaaccaccaa ttaatgaact ggcaaccacc accacggcga caatcattcc ccggcgatta
    13621 aggacaatct gctttaaggt catgccatta ttgcgcaact gaataccgac gaggaaaagt
    13681 agcagaatta aggtgtattc actggcttcg gtcgcgtgtt gtaagaaagc cagtccactc
    13741 agaccaatgg caaaaccaat cactactacg ccgcacagtt ttagcgactc cagcgccatc
    13801 gcaatacgcg acgggagttt ttcttgatga tggtggttgc gccatggcag gccgcgctcc
    13861 agccacatca gggcggcaat attacacagt aaaataacgg taatactgac ggcagaataa
    13921 tgcagaattg ccaacaagtt actggcgagg ttatcgagaa acgccagact gatacccata
    13981 aaaaagagaa taaggtaaac catccaactt aatagctgat taataactct taatgcagct
    14041 ttttggcgaa gcggaatgag gtaacccaca atcaggggaa ccagaatgat taacagccca
    14101 gaaaacatga aaaaccagtc cttgcaaaga agattcgaaa tgcgcgatga cacactactg
    14161 aaagcggaag gacgagtaaa gttgcaatta aaaggaaatg ttatgcatca gagggggatt
    14221 tttaattcta tatgagtgtt tagcattgtt gcgctccttt catgtgcaat
//
LOCUS       DAZFFE010000114        14227 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFFE010000114 DAZFFE010000000
VERSION     DAZFFE010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.114"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            244..1623
                     /gene="ygjG"
                     /locus_tag="ACHJ69_003905"
     CDS             244..1623
                     /gene="ygjG"
                     /locus_tag="ACHJ69_003905"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO5016793.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(1665..1997)
                     /locus_tag="ACHJ69_003906"
     CDS             complement(1665..1997)
                     /locus_tag="ACHJ69_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO5016794.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            2216..3199
                     /gene="ebgR"
                     /locus_tag="ACHJ69_003907"
     CDS             2216..3199
                     /gene="ebgR"
                     /locus_tag="ACHJ69_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO5016795.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTSALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            3383..6475
                     /gene="ebgA"
                     /locus_tag="ACHJ69_003908"
     CDS             3383..6475
                     /gene="ebgA"
                     /locus_tag="ACHJ69_003908"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO5016796.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLATSPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSARF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWENVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFVVEQ
                     SDDEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            6472..6921
                     /locus_tag="ACHJ69_003909"
     CDS             6472..6921
                     /locus_tag="ACHJ69_003909"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO5016797.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            6984..8417
                     /locus_tag="ACHJ69_003910"
     CDS             6984..8417
                     /locus_tag="ACHJ69_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5016798.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLTSAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            8551..9621
                     /gene="ygjJ"
                     /locus_tag="ACHJ69_003911"
     CDS             8551..9621
                     /gene="ygjJ"
                     /locus_tag="ACHJ69_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO5016799.1"
                     /translation="MKLITAPCRALLALPFCYAFSVTADEARPAEHDDSKTPAMTSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLTEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQLFLMQYFYW"
     gene            9638..11988
                     /gene="ygjK"
                     /locus_tag="ACHJ69_003912"
                     /pseudo
     CDS             9638..11988
                     /gene="ygjK"
                     /locus_tag="ACHJ69_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            12214..>14227
                     /gene="fadH"
                     /locus_tag="ACHJ69_003913"
     CDS             12214..>14227
                     /gene="fadH"
                     /locus_tag="ACHJ69_003913"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO5016800.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDANQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVGAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQILAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALE"
BASE COUNT         3463 a         3755 c         3733 g         3276 t
ORIGIN      
        1 ttatcttgcc caaaaatcag gcaattattg ccctgaaagc gtgcatttgc gcagcaatca
       61 tcaaatccat acccgacaaa aaccgtgcaa aataacagca aatgttagca aatagcatta
      121 aatattgcgc aaatgataac cacatttgtg tttatcccga ttttcacgat cgcagccgga
      181 gtggcgcaat ccctgcaata cttaaatcgg tatcatgtga tacgcgagcc tccggagcat
      241 attttgaaca ggttaccttc gagcgcatcg gctttagcgt gcagcgccca cgccctgaat
      301 ctcattgaaa agcgaacgtt ggatcatgag gagatgaaag cacttaaccg ggaggtgatt
      361 gaatacttca aagagcatgt caatccgggg tttttagagt atcgcaaatc tgttaccgcc
      421 ggcggggatt acggagccgt agagtggcaa gcgggaggtt taaatacgct tgtcgacacc
      481 cagggacagg agtttatcga ctgcctggga ggttttggaa ttttcaacgt ggggcaccgt
      541 aatccagttg tggtttccgc cgtacagaat caacttgcga aacaaccgct tcacagccag
      601 gagctgctcg atccgttacg ggcgatgctg gcgaaaaccc ttgcggcgct aacgcccggt
      661 aaactgaaat acagcttctt ctgtaatagc ggcaccgaat ccgttgaagc ggcgctgaag
      721 ctggcgaagg cttatcagtc accgcgcggc aagtttactt ttattgccac cagcggcgcg
      781 ttccacggta aatcacttgg cgcgctgtcg gccacggcga aatcgacctt ccgcaaaccg
      841 tttatgccac tgctgccagg gttccgtcat gtaccgtttg gcaatatcga agccatgcgc
      901 acggctctta acgagtgcaa aaaaaccggt gatgatgttg ctgcggtgat cctcgaaccg
      961 attcagggcg aaggtggcgt aattctgccg ccgccaggct atctcaccgc cgtgcgtaag
     1021 ctgtgcgatg agttcggcgc actgatgatc ctcgatgaag tacaaacggg catggggcgc
     1081 acgggcaaga tgttcgcctg cgagcatgag aacgtacagc cggatatcct ttgtctggca
     1141 aaagcgctcg gtggcggcgt gatgccgatt ggcgcaacca tcgccactga agaggtgttc
     1201 tcagtcctgt tcgacaatcc gttcctgcat accaccacct ttggcggcaa cccgctggcc
     1261 tgtgcggcgg cgctggcgac catcaatgtg ttgctggagc agaacttacc ggctcaggct
     1321 gaacaaaaag gcgatatgtt gctggacggt ttccgtcaac tggcgcggga atatcccgat
     1381 ctggtacagg aagcgcgtgg taaagggatg ttgatggcga ttgagtttgt tgataacgaa
     1441 atcggctata actttgccag cgagatgttc cgccagcgcg tactggtggc cggaacactc
     1501 aacaacgcta aaacgatccg cattgaaccg ccactgacac tgaccattga acagtgtgaa
     1561 ctggtgatca aagcggcgcg taaggcgctg gcggccatgc gagtcagtgt cgaagaagcg
     1621 taatatcaaa tcggatggcg atacgacgtc gccatccgat ctttttatac gatacgtacg
     1681 cccgcaggca tcatccgctc cggcgttaac agcacgcttt cactgccgtc atcggtttcc
     1741 gcacacaaca gcatgcattc cgacgtttca ccgcgcattt tcgctttttg cagattgcat
     1801 aagaccacca ctgttttccc catcagctct tcttcgctgt agtacggcac caggctggtc
     1861 acggtttgca acgttttttc acccacatca acctgtacga tgtacagctt gtcggcgttt
     1921 tcatggcgtt tcacttccac aatctttccg acacgcattt ccagacgtgc aaaatcagcg
     1981 taagccacgg tttccattgt ttcctccctt cgagtaaaat tttactaaac tatagcaaaa
     2041 gtttttctcg atcctgtagg ctaaaaatgg agaatgcagg cgtgatcaca ttcgcaagcc
     2101 gctgtgttac cgttacagcg tcaaagaaac gcgctttatt tactgaaaac aggtgacccg
     2161 ataagcactt cctctacaat gggggcgcac atcagggaaa gtaaaaaagg taaacatggc
     2221 aacactaaaa gacatcgcaa tcgaagctgg cgtatccctg gcgacagtat ccagggtctt
     2281 gaatgacgat ccgacattga atgtgaaaga agagacgaaa catcgcattc tcgagatcgc
     2341 cgaaaagctg gagtacaaga ccagtagcgc ccgtaaactc cagacaggcg cagtcaacca
     2401 acaccatatt ctggctatct acagctacca acaggagctg gagatcaacg atccttacta
     2461 tctggcgatc cgccacggca ttgaaactca gtgcgaaaag ctggcgatcg aactgaccaa
     2521 ctgttatgaa cacaacggct taccagacat taaaaacgtc accggtattt taattgtcgg
     2581 caaacccacg tccgccctgc gcgccgccgc ctgtgcgttg accgacaata tctgttttat
     2641 cgactttcac gaacccggca gcggttacga tgcggtggat atcgatctgg cacgtatcag
     2701 taaagaaatc atcgatttct atatcaatca gggcgttaat cgtattggtt ttattggcgg
     2761 tgaagactta cccggcaagg cggatattcg tgaggtcgcc tttgcggaat atggtcgtct
     2821 gaaacacgtg gtacgcgaag aggatatctg gcgcggcggt ttttccagtt cgtcgggcta
     2881 tgaactggca aaacagatgc tggcgcggga agactatccg aaggcactgt ttgttgcttc
     2941 cgattccatt gctatcggcg tactgcgggc aattcatgaa cgtggcctga acatcccaca
     3001 ggatatttcg cttatcagcg ttaacgatat ccccaccgcg cgatttacct ttccgccgct
     3061 ctccaccgtg cgcatccatt ccgaaatgat gggaagtcag ggcgttaacc tagtgtatga
     3121 aaaagcccgc gatggtcgcg cgctgccgct gttggtcttc gttcccagca aattaaaact
     3181 gcgcggcacg acccgttaaa tccccttgca cgctgtccgg caatcgtttt tgccggacag
     3241 tgctgccgtt tattttcgtg atccagttaa agtaaatgca tttatccgct actttttagt
     3301 aaaaatttta ctaaaccccc cagcaattac acaaactacc atcaccatga atggttccga
     3361 tttctctcta ccgggaggcc ctatgaatcg ctgggaaaac attcagctca cccacgaaaa
     3421 ccgacttgcg ccgcgtgcgt actttttttc atatgattct gttgcgcagg ctcgtacttt
     3481 tgcccgtgaa accagcagcc tgtttctgcc cttaagcggt cagtggaatt tccacttttt
     3541 tgaccatccg ctgcaagtgc cagaagcctt cacctctgag ttaatggctg actgggggca
     3601 tattaccgtc cccgccatgt ggcaaatgga aggtcacggc aaattgcaat ataccgacga
     3661 aggttttccg ttccccatcg atgtgccgtt tgtccccagc gataacccaa ccggtgccta
     3721 tcaacgtatt ttcaccctca gcgacggctg gcagggtaaa cagacgctga ttaaatttga
     3781 cggcgtcgaa acctattttg aagtctacgt taacggtcag tatgtggggt tcagcaaggg
     3841 cagtcgcctg accgcagagt ttgacatcag cgcgatggtt aaaaccggcg acaacctgtt
     3901 gtgtgtgcgc gtgatgcaat gggcggactc tacctacgtg gaagaccagg atatgtggtg
     3961 gtcggcgggg attttccgcg atgtttatct ggtcggtaaa caactcacgc atattaacga
     4021 tttcaccgtt cgtaccgact ttgacgaagc ctattgcgat gccacacttt cctgcgaagt
     4081 ggtgctggaa aatctcgcca cctcccctgt cgtcacgacg ctggaatata ccctgttcga
     4141 cggcgaacgc gtggtgcaca gcagcgccat tgatcatctg gcaattgaaa aactgaccag
     4201 cgcccgcttt gcttttactg tagaacagcc gcagcaatgg tcagcagaat ccccttatct
     4261 ttaccatctg gtcatgacgc tgaaagacgc cgacggtaac gttctggaag tggtgccaca
     4321 acgcgttggc ttccgtgata tcaaagtgcg cgacggtctg ttctggatca ataaccgtta
     4381 tgtgatgctg cacggcgtca accgccacga caacgatcat cgcaaaggcc gcgccgttgg
     4441 gatggatcgc gtcgagaaag atctccagtt gatgaagcag cacaacatca actccgtgcg
     4501 taccgctcac tacccgaacg atccacgttt ttacgaactg tgtgatatct acggcctgtt
     4561 tgtgatggcg gaaaccgacg tcgaatcgca cggctttgct aacgtcggcg atatcagccg
     4621 cattaccgac gatccgcagt gggaaaacgt ctacgtcgag cgcattgttc gccatattca
     4681 cgcgcagaaa aaccatccgt cgatcatcat ctggtcgctg ggcaatgaat ccggctatgg
     4741 ctgtaacatc cgcgcgatgt accacgcggc gaaggcgctg gatgacacgc gactggtgca
     4801 ttacgaagaa gatcgcgatg ctgaagtggt cgatattatt tccaccatgt acacccgcgt
     4861 gccgctgatg aatgagtttg gtgaataccc gcatccgaag ccgcgcatca tctgtgaata
     4921 tgctcatgcg atggggaacg gaccgggcgg gctgacggag taccagaacg tcttctataa
     4981 gcacgattgc attcagggac attatgtctg ggaatggtgc gaccacggga tccaggcgca
     5041 ggatgacaac ggcaacgtct ggtataaatt cggcggcgac tacggcgact atcccaacaa
     5101 ctataacttc tgtcttgatg gtttgatcta ttccgatcag acgccgggac caggcctgaa
     5161 agagtacaaa caggttattg cgccggtaaa aatccacgcg ctggatctga ctcgcggcga
     5221 gttgaaagtc gaaaataaac tatggtttac cacgcttgat gactacaccc tgcacgcaga
     5281 ggtgcgcgcc gaaggtgaaa cgctcgcgac gcaacagatt aaactgcgcg acgttgcgcc
     5341 gaacagcgaa gcccccttac agatcacgct gccgcagctg gacgcccgcg aagcgttcct
     5401 caacattacg gtgaccaaag attcccgcac ccgctacagc gaagccgggc attctatcgc
     5461 cacttatcag ttcccgctga aggaaaacac cgcgcagcct gtacctttcg caccgaataa
     5521 cgcccgtccg ctgacgctgg aagacgatcg tttgagctgt actgttcgcg gctacaactt
     5581 tgcgatcacc ttctcaaaaa cgagtggtaa accgacatcc tggcaagtga atggcgagtc
     5641 gctgctgacc cgcgagccaa agatcaactt cttcaagccg atgatcgaca accataagca
     5701 ggagtacgaa gggctgtggc agccaaatca tttgcagatc atgcaggagc atctgcgcga
     5761 ttttgtcgtt gagcaaagtg atgatgaagt gttgatcatc agccgcacgg ttattgcccc
     5821 accggtgttt gatttcggaa tgcgctgcac ctacatctgg cgcatcgctg ccgatggcca
     5881 ggttaacgtg gcgctttccg gcgagcgtta cggcgactat ccgcacatca ttccgtgcat
     5941 cggtttcacc atggggatta acggcgaata cgatcaggtg gcgtattacg gtcgtggacc
     6001 gggcgaaaac tacgccgaca gccagcaggc taacatcatc gatatctggc gcagcaccgt
     6061 cgatgccatg ttcgagaact atcccttccc gcagaacaac ggtaaccgtc agcatgtccg
     6121 ctggacggca ctgactaacc gccacggcaa cggtctgctg gtggttccgc agcgaccaat
     6181 taacttcagc gcctggcact atacccagga aaacatccac gctgcacagc actgtaacga
     6241 gctgcagcgc agtgatgaca tcactctgaa tctcgaccac cagctgcttg gcctcggctc
     6301 caactcctgg ggcagcgagg tgctggactc ctggcgcgtc tggttccgtg acttcagcta
     6361 cggctttacg ttgctgccgg tttctggcgg agaagctacc gcgcaaagcc tggcgtcgta
     6421 tgagttcggc gcagggttct tttccacgaa tttgcacagc gagaataagc aatgaggatc
     6481 atcgataact tagaacagtt ccgccagatt tacgcctctg gcaagaagtg gcaacgatgc
     6541 gttgaagcga ttgaaaatat cgacaacatt cagcctggcg tcgcccactc catcggtgac
     6601 tcattgactt accgcgtgga gacagactcc gcgaccgatg cgctatttac cgggcatcga
     6661 cgctattttg aagtgcatta ctacctgcaa gggcagcaaa aaattgaata tgcgccgaaa
     6721 gagacattac aggtagtgga atattatcgt gatgaaactg accgtgaata tttaaaaggc
     6781 tgcggagaaa ccgtagaggt ccacgaaggg caaatcgtta tttgcgatat ccatgaagcg
     6841 tatcggttta tctgcaataa cgcggtcaaa aaagtggttc tcaaagtcac catcgaagat
     6901 ggttatttcc ataacaaata acaactacgg cggcaaaagg agtttgccgc caccgctacc
     6961 ctactcattt tcggagatgt gttatgtctg ataccaaacg taatacaatc ggcaaattcg
     7021 gcttgctctc gctgactttt gccgccgttt acagctttaa caacgttatc aataataata
     7081 ttgagcttgg actgacctcg gcaccgatgt ttttcctcgc gacgattttt tattttattc
     7141 ccttctgtct gatcattgca gaatttgttt cgttaaataa aaactcagaa gccggtgtct
     7201 acgcgtgggt aaaaagttcg ctgggcggac gttgggcatt tattactgcc tatacctact
     7261 ggttcgtaaa cctgttcttt ttcacctcgc tgttaccgcg cgttattgct tatgcttcgt
     7321 atgccttcct cggctacgaa tatattatga cgccggttgc caccaccatt atcagtatgg
     7381 tgctgttcgc cttctccacc tgggtttcta ccaacggggc gaaaatgctg gggccaatta
     7441 cctccgtcac ttcaacgctg atgctgctgt taacgctctc ctacattttg ctggcaggaa
     7501 cggcactggt tggcggcgta cagcctgctg accccatcac tgttgacgcg atgatcccaa
     7561 actttaactg ggcgttcctc ggcgttacca cctggatctt tatggccgca ggtggcgcgg
     7621 agtccgtcgc tgtgtacgtt aacgacgtca aaggcggttc gaaatcgttc gttaaagtga
     7681 tcatcctcgc cgggattttt atcggcgtac tgtattccgt ctcctcggtg ctgattaacg
     7741 tcttcgtcag cagcaaagag ttgaaattta ctggcggatc ggtacaggta ttccacggca
     7801 tggcggcgta ttttggtcta ccggaagcac tgatgaatcg ctttgtcggt ctggtgtcct
     7861 ttaccgcgat gttcggttcc ctgctgatgt ggacggcaac gccggtgaaa attttcttct
     7921 ccgaaatccc ggaaggcatc tttggtaaga aaaccgtcga actgaacgaa aacggcgttc
     7981 cggcgcgcgc agcgtggatc cagttcctga tcgtcatccc gctgatgatt atcccgatgc
     8041 tcggttccaa taccgtgcag gatctgatga atactattat taatatgact gccgcagcgt
     8101 ccatgcttcc gccgttattc atcatgctgg cttacctgaa tttacgcgcc aaattagatc
     8161 acctgccacg cgatttccgt atgggctccc gccgcaccgg tattatcgtt gtttcaatgc
     8221 tgattgcgat atttgccgta ggatttgtcg cttcgacatt cccgactggc gcgaatattc
     8281 tgaccatcat tttttataac gtcggcggta ttgttatctt cctcggcttt gcgtggtgga
     8341 aatacagtaa atatataaag ggattaacgg ctgaagagcg ccatattgaa gcgacgccag
     8401 ccagcaatgt tgattaagca gaataataaa aaatgagcag gcattttccc tcttcctgga
     8461 tggggagagg gaaaatagtt tctgccttta tatttttata attacaacga taaaaggctg
     8521 tactttttct ttagctcatg gattaacaca atgaaattaa tcactgcacc atgcagagca
     8581 ttacttgcgc tgccgttttg ctacgccttt tctgtgaccg ctgatgaagc acgtccggca
     8641 gaacatgacg actcaaaaac ccccgcaatg acctcgacat cttctccttc atttcgtttt
     8701 tacggcgaat taggggttgg tggatatatg gatttagagg gtgagaataa acataaatac
     8761 agcgacggca cctatattga aggtggcctg gagatgaagt acggctcctg gttcggcctg
     8821 atttacggcg aaggctggac cgtacaggcc gaccacgacg gtaatgcctg ggtgcctgac
     8881 catagctggg gtggcttcga aggtgggatt aaccgtttct atggcggtta tcgtaccaat
     8941 gatggcaccg aaatcatgct cagtctgcgt caggattcct cgctggatga cctgcaatgg
     9001 tggggcgatt tcacgcccga tctaggctac gtcattccca atacccgcga tattatgact
     9061 gcgctgaagg tacagaactt aagcggcaac tttcgttata gcgttaccgc cacacctgcc
     9121 ggacatcatg acgaaagcaa agcctggcta cattttggca agtacgatcg ctacgacgac
     9181 aaatatacct atccggcaat gatgaacggt tacatccagt atgaccttac agaaggtatc
     9241 acctggatga atggtctgga aatcaccgac ggcacaggcc aactctatct cacgggcctg
     9301 cttaccccta actttgccgc tcgcgcctgg caccataccg gacgcgccga cgggctggac
     9361 gtaccgggaa gtgaaagcgg gatgatggtg agcgccatgt atgaagcgtt aaagggcgtt
     9421 tatctctcca ccgcttacac ctacgccaaa catcgccctg accacgctga cgatgaaacc
     9481 acctctttca tgcagtttgg tatctggtac gaatacggcg gcggacgttt cgcaacggct
     9541 tttgatagcc gcttctacat gaaaaatgcc tctcacgatc ccagcgacca actcttcctg
     9601 atgcaatatt tttactggta ataaggacag taatctcatg aaaatcaaaa ccattttaac
     9661 gccagtagcc tgtgctctgc tgatgagctt ttccgcccat gccgctaacg ctgacaatta
     9721 taaaaacgtg attaaccgta ctggcgcgcc gcagtacatg aaggattacg attacgacga
     9781 tcaccagcgt tttaatccgt ttttcgatct cggagcctgg catggtcatc tgttgccaga
     9841 cggccctaac accatgggcg gttttccagg cgttgcgctg ctgacggaag aatacatcaa
     9901 ctttatggcc agcaatttcg atcgcctgac cgtctggcaa gacggcaaga aagtcgattt
     9961 cacgctggag gcatacagta tacccggcgc gctggtgcaa aagttgatat cgaaagatgt
    10021 acaggtcgaa atgattctgc gcttcgccac gccgcgcaca tcactactgg aaaccaaaat
    10081 caccagcgat aaaccgctgg atctggtgtg ggatggtgaa ctgctggaaa aactggaagc
    10141 gaaagaaggg aaaccgcttt ccgataaaac cattgctggc gagtaccctg actatcagcg
    10201 caaaatcagc gccacccgtg atggcctgaa agtcatcttt ggcaaagtgc gcgccacctg
    10261 ggatctgctg acctccggtg aatcagaata tcaggtgcat aaatccctgc cagtgcagac
    10321 tgaaatcaac ggcaatagct ttaccagtaa ggcgcatatc aacggttcga cgacgctcta
    10381 taccacctac tcccatctgc tgaccgctca ggaagttagc aaagagcaaa tgcagatccg
    10441 cgatattctg gcgcgtccgg cgttttatct cactgcctca cagcaacgct gggaagagta
    10501 tctgaagaaa gggttaacca atccggatgc gacgccggag cagacgcgcg tcgcggtgaa
    10561 agcatcgaaa cgctcaacgg taactggcga tcgccgggcg gtgcggtgaa atataacacc
    10621 gtcacgccgt cggtgaccgg gcgctggttc tccggcaatc agacctggcc gtgggatacc
    10681 tggaagcagg cgtttgcgat ggcgcatttc aatccggata tcgccaaaga gaatatccgc
    10741 gcggtcttct cctggcagat ccagccaggc gacagcgtgc gtccgcagga tgtgggcttt
    10801 gtccccgacc tgatagcgtg gaaccttagc ccggagcgtg gcggcgatgg cggcaactgg
    10861 aacgaacgta acaccaaacc cagccttgcc gcctggtcgg tgatggaagt gtacaacgtc
    10921 acccaggata aaaactggct ggcagagatg tacccgaaac tggtggccta tcacgactgg
    10981 tggctacgta accgcgatca caacggcaac ggcgtaccgg aatatggcgc aacccgcgat
    11041 aaagcccaca atactgataa tggcgagatg ctgtttacag tgaaaaaagg caataaagaa
    11101 gagacgcagt ctgggctgaa caactacgcc cgcgtggtgg agaaaggcca gtacgacagt
    11161 ctggaaattc cggcacaggt tgcggcgtcg tgggaatcgg ggcgtgatga tgccgccgtc
    11221 tttgggttta tcgacaaaga acagttggat aaatatgtcg ctaacggcgg caaacgtagc
    11281 gactggacgg tgaaattcgc cgagaaccgc agtcaggacg gaacgttgct gggctactcg
    11341 ctattgcagg agtcggtgga tcaggccagc tatatgtaca gcgataacca ttatctggcg
    11401 gagatggcga cgattctcgg taagccggaa gaggccaaac gctatcgcca gttggcacaa
    11461 cagctcgcgg actacatcaa cacttgtatg ttcgacccga ctacacagtt ctactatgac
    11521 gtgcgtattg aagataaacc gctggcgaac ggctgtgcgg gcaaaccgat tgtcgagcgc
    11581 ggtaaagggc cggaaggctg gtcaccgctg tttaacggtg cggcaacgca ggccaacgcc
    11641 gacgcggtgg tgaaggtgat gctggatccg aaagaattca acacctttgt cccgctgggg
    11701 acggcagcgt taaccaaccc agcctttggc gcagatatct actggcgcgg gcgcgtatgg
    11761 gtggatcagt tctggtttgg tctgaaaggg atggagcgtt acggttatcg cgatgacgcc
    11821 ctgaaactgg ctgatacgtt cttccggcac gccaaaggat taaccgccga tggcccaatt
    11881 caggagaatt acaacccgct cactggcgcg cagcaaggcg caccaaattt ctcctggagt
    11941 gccgcgcatt tgtatatgtt gtataacgat ttttttcgta agcagtaatt agcaacggct
    12001 ggcggatgcg gcgtaaacgc cttatccgcc ctacatgtgc aatccccctg gccggataag
    12061 acgcgtcaag cgtcgcatcc ggcgttacca ccgggcgtat tctttttgaa tcccatcaca
    12121 aaccccgcac tccccttttc ccttttctcc ggcgacggct aaattagaac tcatccgacc
    12181 acataacaat tattttacat actggacaat tttatgagct acccgtcgct gttcgccccg
    12241 ctggatttag gttttaccac gttaaaaaac cgcgtgttga tgggctcaat gcacaccggg
    12301 ctggaggaat acccggacgg cgctgaacgg ctggcagcgt tttatgccga acgcgcccgt
    12361 cacggcgtgg cgttgattgt cagcggcggt atcgcaccag atttaacagg cgttggcatg
    12421 gaaggcggtg caatgctcaa tgacgccaac cagatcccac accatcgcac cattaccgaa
    12481 gccgtacatc aggaaggcgg caaaatcgcc ctgcaaattt tgcataccgg gcgctacagc
    12541 taccaaccgc atctggtcgc tccctccgca ttgcaggccc ccatcaaccg tttcgttcct
    12601 cacgaactca cccatgaaga gatcctgcaa ctgatcgacg atttcgccca ctgcgcgcaa
    12661 ttggcacggg aggcaggata cgacggtgta gaggtgatgg ggtccgaagg gtatttgatc
    12721 aacgaatttc tgacgctgcg caccaatcag cgtagtgacc agtggggcgg cgattaccgc
    12781 aaccggatgc ggtttgccgt agaagtagtg ggtgcggtgc gcgaacgtgt cggcaacgac
    12841 ttcattatta tctaccgttt gtcgatgctc gacctggtcg aaggcggcgg tacttttgcc
    12901 gaaacggtag agctggcgca ggccattgaa gcggcgggcg caacgattat caacaccggc
    12961 attggctggc atgaagcacg tattccgacc attgccacac ccgtgccgcg cggcgcattt
    13021 agctgggtca cgcgcaaact gaaaggccac gtttcgctgc cactggtgac caccaaccgg
    13081 attaacgatc cgcaggttgc cgacgacatt ctctcgcgcg gtgatgcgga catggtatcg
    13141 atggcgcgac cgtttctcgc tgatgcggag ctgctgtcaa aagcgcaatc gggacgagcc
    13201 gatgagatca acacttgtat tggctgcaac caggcctgtc tcgatcagat cttcgttggc
    13261 aaagtcacct cgtgcctggt gaatcctcgc gcctgccatg aaaccaaaat gccaatcctt
    13321 cctgccgtgc agaaaaaaaa tctggcggtg gtcggtgcgg gacctgctgg gctggcgttt
    13381 gccattaacg cggcggcgcg tgggcatcag gtaacattgt ttgatgcgca cagcgagatc
    13441 ggcgggcagt ttaatatcgc caaacagatc cccggcaaag aggagtttta cgaaacgctg
    13501 cgctattacc gccggatgat cgaagtgacg ggtgtgacgc taaaactcaa tcacaccgtg
    13561 acagcggatc agttacaggc gttcgatgaa acgatcctcg ccagtgggat cgtgccgcgc
    13621 gttccgccta tcgacgggat cgatcatccg aaggtattga gttatctcga tgtactgcgc
    13681 gacaaagcgc cggtgggcaa caaagttgcc atcatcggtt gtggcggaat tggttttgat
    13741 acggcgatgt atttaagtca gccgggagaa tccaccagcc agaatatcgc cgggttctgt
    13801 aatgagtggg gaatcgacag tagcctgcaa caggccggag gtttgagtcc gcaaggcatg
    13861 caaatccccc gtagcccacg gcagattgtg atgctccagc gcaaagccag caaaccagga
    13921 caagggttag gtaaaaccac cggctggatc catcgcacca ccctgctctc acgcggcgtg
    13981 aaaatgatcc caggcgtaag ttaccagaag atcgacgatg acgggctgca tgtagtgatc
    14041 aacggcgaaa cgcagatatt agcagtggac aatgtggtga tctgcgcagg gcaagagcca
    14101 aaccgcgcat tggcgcaacc gctgattgat agtgggaaaa ctgtgcattt aattggcggc
    14161 tgcgatgtag ctatggagct ggacgcacgg cgggcaattg cccagggaac acggctggcg
    14221 ctggaga
//
LOCUS       DAZFFE010000115        13833 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFFE010000115 DAZFFE010000000
VERSION     DAZFFE010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.115"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1435
                     /locus_tag="ACHJ69_003914"
     CDS             <1..1435
                     /locus_tag="ACHJ69_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO5016801.1"
                     /translation="NQSATDTLPSPVPETDAVETAIPGNEKTECKVEVEPSVEREGPF
                     YFLFTDKDGEKYGRANKLSGLEKALALGATEITKEEYFARKNGTYTGSQQNTDASDTT
                     AQPDLVKVTADEVNKIMQAASISQPDADKLLAASRGEFVAGISDPNDPKWVKGIETRD
                     SVDQNQQESEQNDQKAEQNSPNALQNEPETKQPEPVVQQEPEKICTACGQTGGGNCPD
                     CGAVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENAGSAQDHASDSETGETAD
                     PLITVNGHHVITSTSRLWHHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKT
                     IDLETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQV
                     WGNGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTATPFEGERH
                     NALDDARYQAKYVSAIWQKLIPSQADF"
     gene            1503..1745
                     /locus_tag="ACHJ69_003915"
     CDS             1503..1745
                     /locus_tag="ACHJ69_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO5016802.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            1723..2742
                     /locus_tag="ACHJ69_003916"
     CDS             1723..2742
                     /locus_tag="ACHJ69_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5016803.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRTIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRSA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            3152..3811
                     /gene="yccA"
                     /locus_tag="ACHJ69_003917"
     CDS             3152..3811
                     /gene="yccA"
                     /locus_tag="ACHJ69_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO5016804.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            3903..4232
                     /gene="tusE"
                     /locus_tag="ACHJ69_003918"
     CDS             3903..4232
                     /gene="tusE"
                     /locus_tag="ACHJ69_003918"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO5016805.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGIALSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(4229..4507)
                     /gene="yccX"
                     /locus_tag="ACHJ69_003919"
     CDS             complement(4229..4507)
                     /gene="yccX"
                     /locus_tag="ACHJ69_003919"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO5016806.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLTGYAKNLDDGSV
                     EVVACGDEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            4602..5792
                     /gene="rlmI"
                     /locus_tag="ACHJ69_003920"
     CDS             4602..5792
                     /gene="rlmI"
                     /locus_tag="ACHJ69_003920"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO5016807.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFTRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPACAIYDRSDVA
                     VRKKEGMELTQGPITGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            5850..6167
                     /gene="hspQ"
                     /locus_tag="ACHJ69_003921"
     CDS             5850..6167
                     /gene="hspQ"
                     /locus_tag="ACHJ69_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO5016808.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(6212..6625)
                     /gene="yccU"
                     /locus_tag="ACHJ69_003922"
     CDS             complement(6212..6625)
                     /gene="yccU"
                     /locus_tag="ACHJ69_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO5016809.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            6798..7455
                     /gene="yccT"
                     /locus_tag="ACHJ69_003923"
                     /pseudo
     CDS             6798..7455
                     /gene="yccT"
                     /locus_tag="ACHJ69_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
     gene            7551..8009
                     /gene="mgsA"
                     /locus_tag="ACHJ69_003924"
     CDS             7551..8009
                     /gene="mgsA"
                     /locus_tag="ACHJ69_003924"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO5016810.1"
                     /translation="MELTTRTLPSRKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(8041..10095)
                     /gene="helD"
                     /locus_tag="ACHJ69_003925"
     CDS             complement(8041..10095)
                     /gene="helD"
                     /locus_tag="ACHJ69_003925"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO5016811.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGMRQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIEGARLQHNQAYTEAMLIEYADFFRQVESSPLNPAQARAVVNCEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLAFRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDEGQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVDILKNLDVPVAR
                     KP"
     gene            10218..10664
                     /gene="yccF"
                     /locus_tag="ACHJ69_003926"
     CDS             10218..10664
                     /gene="yccF"
                     /locus_tag="ACHJ69_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO5016812.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFQ"
     gene            10674..12836
                     /gene="yccS"
                     /locus_tag="ACHJ69_003927"
     CDS             10674..12836
                     /gene="yccS"
                     /locus_tag="ACHJ69_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO5016813.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVLA"
     gene            complement(12799..13428)
                     /gene="sxy"
                     /locus_tag="ACHJ69_003928"
     CDS             complement(12799..13428)
                     /gene="sxy"
                     /locus_tag="ACHJ69_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO5016814.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            13647..>13833
                     /locus_tag="ACHJ69_003929"
     CDS             13647..>13833
                     /locus_tag="ACHJ69_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulA-like leucine-rich domain-containing
                     protein"
                     /protein_id="HFO5016815.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSR"
BASE COUNT         3488 a         3506 c         3353 g         3486 t
ORIGIN      
        1 aaatcagagc gcaactgata ctctcccgtc gccagtacca gaaactgatg cagtggaaac
       61 ggcaattccg ggcaacgaaa aaaccgaatg caaagtggaa gtcgaaccat ctgtagagcg
      121 tgaggggccg ttctacttcc tcttcaccga caaggatggc gaaaaatacg gtcgcgcaaa
      181 caaactttct ggtctggaaa aagcactagc cttgggagct acggaaatca caaaagagga
      241 atacttcgca cgcaaaaacg gcacatacac aggttcacaa caaaatactg atgcatctga
      301 cacgaccgca caaccagacc tggtaaaagt taccgctgac gaagtaaaca aaattatgca
      361 ggcagccagt atcagccagc ctgacgccga taagttgctt gctgcctctc gcggagaatt
      421 tgttgcaggg attagcgacc cgaatgatcc gaaatgggta aaggggattg aaacccgcga
      481 ttctgtagac cagaaccagc aagaatcgga acagaacgac cagaaagcgg aacaaaacag
      541 cccaaatgcg ttacaaaacg agccagaaac gaaacaacct gaaccagtag tgcaacagga
      601 accggaaaag atctgcaccg cctgcggtca gaccggcggc ggcaactgcc ctgattgtgg
      661 cgcggtgatg ggcgacgcaa cataccagga aacattcgat gaagagtatc aggttgaagt
      721 tcaggaagat gatccggagg aaatggaagg cgctgaacat ccacacaagg agaatgctgg
      781 cagcgctcag gatcacgcca gcgatagtga aactggcgag acggcagatc ccttaattac
      841 ggtgaacggt catcacgtta tcacatccac cagcaggttg tggcaccatc tgatgattga
      901 ccttgaaacc atgggaaaaa atcctgatgc cccgatcatc tcaataggtg caatattttt
      961 cgatccgcaa accggagata tgggaccgga atttagtaag actatcgatc tggaaactgc
     1021 tggcggggtc attgatcggg acaccattaa atggtggctt aagcaatcac gcgaagcgca
     1081 atctgccatt atgaccgatg aaatcccgtt agatgatgca ctgttacaat tgcgggaatt
     1141 tatcgacgaa aactccggtg aattttttgt tcaggtctgg ggaaatggag ccaacttcga
     1201 caacacgatt ttgcgccgtt catacgaacg gcaggggatc ccctgcccgt ggcgttacta
     1261 caacgatcgc gatgtacgca caatcgttga gctggggaaa gccatagact tcgatgccag
     1321 aactgctacc ccattcgaag gtgagcgcca taatgcactt gatgacgccc gttatcaggc
     1381 aaaatacgtt tcagctatct ggcaaaaact gatcccgagt caggctgatt tttaatgttc
     1441 aacccatatc gccgcccacc agctatagtg gcggcggtca tgctgtaaag gcacgtgacc
     1501 acatgtacga attaactcta tctccagcag agattaaaga gatcacgaaa tacgagcgat
     1561 acacaaaaca gcaacaccag ttaagactgc acggcatccc atttgtaatc ggtcctaaaa
     1621 acgaaccaat agttctacgc agggatattc cacacggact gacaacgatg ccaaaaacat
     1681 ctgaactggt ttctgctgaa cccgattttg aggcactgaa caatgggaag accaagaaaa
     1741 aataaaaaag ataatgtact gccaccgcgg gttagatcga atggttacag ttacgtgtgg
     1801 aaaccagaag gaagtacaag aactataggg ctaggaagag tgcggaaaac cagcgtagct
     1861 aaagtctggc aaaattatga actggaaaaa gcaaaactcc acaacataat gaccgtagct
     1921 aaattatggc acatgtttat ggactcccct gcatttacag aactggcccc ccgaacccaa
     1981 aaagattatc gacaacatca gaaggcgttg ctgatggtat tcggaaaagt gcttgctgat
     2041 aatgtcaaaa ctgagcaggt aagaattttc atggataaac gagggcttga gagcaagacc
     2101 caggcaaatc atgaactggc aagcctgagt cgagtatacg ggtggggata tgagcgtgga
     2161 tacgtgaaga ataacccatg taaaggagtc agaaaattct ctcttaaagc ccgcactgtt
     2221 tacatcaccg atgaacagta tgcagcgata tatgcggaag caattccaca gttacgcatt
     2281 gcaatggaga tttcctatct ctgtgcggca aggctcggtg atgtgcttga gttgaaatgg
     2341 caggatatta tggataaagg gatctacatt gagcaaaaca aaaccggcac caaacaaatc
     2401 aaggaatggt caccgcgatt acgtacagcg atccagttag cccgaaatgt atcttcctgt
     2461 acatgcgaat atgtgatcaa tacaaccaaa ggcgggaaag tcatagctaa aacgctgaac
     2521 aactggtgga atcaggctaa acgctcagcc gagcaaaaag ttggcgttcc gttcgggtgc
     2581 aattttcacg acataaaagc caaggggatc tcagattacg aaggcagcag tcgcgacaaa
     2641 caaattttca gcgggcataa aacagaaaat caggtgttga tttacgatcg taaaacaaaa
     2701 atcacaccaa cactggatct gccgctcgtg gttagcaagt aggcaataca gaaaattttc
     2761 tggcaaaaat acttggtata ctagctatga gcaacttcct aacaccttga tttttcttgg
     2821 tgttaaattt aattaaattt tcagcagatt cacgtattac actgattttc atttgttttt
     2881 cttcggtctt gaaaaccggc gacccgaaag ggttccagag ttcgaatctc tgcgcttccg
     2941 ccaaataaga taaggggtta gctaaatgct aacccctttt tcttttgcct gtcgaaattc
     3001 tcagggcgtt atatcactgc ctaatgacct gagactccgc tgtgaaactt gtttcagatc
     3061 aaatgcgtaa agatgggtaa aacttctgtg taccttccca cattatcatt atgctgctta
     3121 attaattaca tctgtcatag agagtgactc aatggatcgt atagttagtt cttcacatga
     3181 ccgtacatcg ctgcttagca cccataaggt gctgcgtaat acctattttc tgctgagcct
     3241 gacgctggcc ttttcggcga ttaccgcaac tgccagtacg gtgctgatgc tgccatctcc
     3301 gggtctgatt ctgacgctgg tgggtatgta tggtttgatg ttcctgacct acaaaacggc
     3361 gaataagccg accgggatta tctccgcatt cgcctttacc ggttttctgg gttatatcct
     3421 cggaccaatt ctgaacacct atctgtctgc cggaatgggt gacgtaatcg ctatggcact
     3481 gggcggaacg gcgttggtgt tcttctgctg ctctgcgtat gtgctgacca cccgcaaaga
     3541 tatgtcgttc ctcggcggta tgctgatggc gggtattgtg gtggtgctga ttggtatggt
     3601 tgctaatatc ttcctgcaac tgcctgctct gcatctggcg atcagcgcgg tcttcattct
     3661 gatctcctct ggcgctattt tgtttgaaac cagcaacatc attcatggcg gtgaaacgaa
     3721 ctatattcgt gccacggtta gcctgtatgt ttcgctgtac aacatcttcg tcagcctgct
     3781 gagcattctg ggcttcgcca gccgcgatta atctcgccca gggttactta ccgagcctcg
     3841 ctttatgcgg ggctttgttt tttgttacac tgccgccagt ctgattaagg tagtaaagaa
     3901 ttatgctgat cttcgaaggt aaagagatag aaacggatac cgaaggctat ctcaaagaaa
     3961 gcagccagtg gagtgaacca ctggcggtgg tgattgcaga gaacgaaggg attgcgctgt
     4021 cgccagaaca ctgggaagtg gtgcgttttg tgcgtgattt ctatctggaa ttcaatactt
     4081 ctccggcgat tcgtatgctg gtaaaagcga tggcgaataa atttggcgaa gaaaaaggta
     4141 acagccgcta tctgtaccga ctgttcccga aaggtccggc aaagcaagcc accaaaattg
     4201 ctggcctgcc taaaccggta aaatgcattt aacgaatacg aaaatccgtt aactccccgg
     4261 agggatgatg cggctcgcta agcacccgtt ccacacgcgc tgaacgcggg ccgccacttt
     4321 ttaaccactg cattaatttt tccacctgcc cttcgtcacc gcaggcaacc acttccacgc
     4381 tgccatcgtc aagatttttg gcgtacccgg ttagtcccag ttttttcgct tcgtactgcg
     4441 tggtgtagcg gaatcctacg ccctgaactc gcccgtaaac ccaagcaatt atgcagactt
     4501 tcgacattgc tgctctcctt atccttaatg gacgcgttgc aaatccatgg aaaactccgg
     4561 acaatgacgc ccatttcttt gcatcgacag aaaagtaaat tatgagtgta cgtttagtgt
     4621 tagccaaagg gcgcgaaaaa tcattacttc gtcgccatcc gtgggtcttt tccggggccg
     4681 ttgcccgcat ggaaggtaaa gccagcctcg gtgaaaccat cgatattgtt gatcatcagg
     4741 gaaaatggtt agcacgcggc gcttattcgc cagcttcgca aatccgggcg cgcgtctgga
     4801 cgtttgaccc gtcagagtct atcgacattg ctttttttac ccgccgttta cagcaagcac
     4861 aaaaatggcg tgactggctg gcgcaaaaag atggcctcga cagctatcgt ttaatcgccg
     4921 gagaatctga tggcctgccg ggtattacta tcgatcgttt cggtaatttt ctggtgctgc
     4981 aactgctgag tgctggcgca gagtatcagc gcgcggcatt aattagtgcc ctgcaaacgc
     5041 tgtacccggc atgtgctatt tacgatcgca gcgacgtcgc ggtacgtaaa aaagaaggga
     5101 tggagctgac tcagggcccc atcaccggcg agttgccgcc tgccctgctg ccgattgaag
     5161 aacacggcat gaaactgctg gtggatattc agcacggaca caaaacgggc tactacctgg
     5221 accagcgtga cagccgcctg gctacccgcc gctacgtcga aaataaacgc gtacttaact
     5281 gtttctccta taccggtggt ttcgccgtat cggcattgat gggcggttgc agccaggttg
     5341 tcagcgttga tacctcccag gaagcgctgg atattgcacg gcagaacgtt gagctgaaca
     5401 aactggatct gagcaaggca gagtttgtcc gtgatgatgt ctttaaattg ctgcgtactt
     5461 atcgcgatcg cggtgaaaaa tttgatgtta tcgtgatgga cccgccgaag tttgttgaga
     5521 ataaaagcca gttgatgggc gcgtgtcgtg gttataaaga catcaacatg ctggcgattc
     5581 agttgctgaa tgaaggcggt attctcctga ctttctcctg ttccggtctg atgaccagcg
     5641 atttatttca gaaaatcatc gcggatgccg caattgatgc cggtcgtgat gtacaattta
     5701 tagagcagtt ccgtcaggca gccgatcatc cggtgatcgc tacctacccg gaagggctat
     5761 atctgaaagg gtttgcctgt cgcgtcatgt aacttgaaat gtggaatttt acccttatat
     5821 caaaggtaaa gatttcccgg gaggtgacta tgattgccag caaattcggt atcggccagc
     5881 aggtccgcca ttccctgtta ggttatctcg gagtggtcgt ggatatcgac ccggtttatt
     5941 cgctctctga accatcgcct gatgagctgg cggtgaatga cgagcttcgc gccgctccgt
     6001 ggtatcacgt ggtgatggag gatgataacg gcctaccggt tcatacctac ctggctgaag
     6061 cccagctaag tagcgaattg caggatgaac atcctgaaca gccatccatg gatgagctgg
     6121 cgcagaccat ccgcaaacaa ctccaggccc cgcgtctgcg taactaatcc ctgaacaaat
     6181 ccccggaagg tttttgcctt ccggggattt tttatttggc caggcccagg cgagggatct
     6241 caatcgccgg gcagcgatcc atcaccacgt ttagccctgc atcacgcgcc agcactgccg
     6301 cttgctcatt aatcaccccc agttgcatcc agagggtttt cgcgccaatg gcgatagctt
     6361 cctgcgccac gccccacgcc gcttctgaat tacggaaaac atcgaccata tcgacttttt
     6421 ctggaacgtc tgccagcgtg ccatacccct gctggcccag cagtgttttg ccggcaactt
     6481 ttggcgaaac aggaatgacg tgatacccct ggtcgagtag atatttcatc acgcgatagc
     6541 tgggacgatc gggtttgtcg cttgccccca ccagcgcaat ggtgtgagtg gacgttaaaa
     6601 tgcctgcaat atcggtttct ttcattattt cctccagact gtttaacaaa gtgtacgaca
     6661 aagccgacat gccaaccatg cagctaatac gaatgaatga gagcaaaagc gggaaatatg
     6721 tctatatgtt agtaagtatg attaaagaaa aattttgaca cattattatt caggcggtct
     6781 tttaacagga gctatctatg aaaaccggca tcgtgaccac cttgattgca ttatgtttgc
     6841 cggtatccgt ttttgccacc acgctgcggc tttctactga tgtcgatctt ctggtactcg
     6901 acggtaagaa ggtttccagc tctctgctgc gcggtgcgga cagcattgaa ctggacaatg
     6961 gaccgcatca gttagtgttt cgcgttgaga agacgattca tctttccaat agtgaagaac
     7021 ggctctatat ctcccctcca ctggtggtta gttttaatac ccagctcatc aaccaggtca
     7081 attttcgcct gcctcgcctg gagaatgagc gggaagctaa ccattttgat gccgcgcctt
     7141 gaattgttgg atggtgatgc gacgccgatt ccggtaaagc tggatattct cgccattacc
     7201 tcaactgcaa aaacgattga ttatgaggtc gaggttgaac gctataacaa atccgcgaaa
     7261 cgcgcttcac taccgcaatt tgccacgatg atggcagatg acagtacgct gctttcgggt
     7321 gtttccgagc tggatgctat tccgacgcag tctcaggtgc tcacagaaca acggctgaag
     7381 tattggttta aactggctga cccacaaacg cgaaatactt tcctgcaatg ggcggaaaaa
     7441 caaccatcct cctgagattt ttgtgcctgt gcgcaggctt tttcagtctt tatcttgcag
     7501 cgataagtac ttacagtaat ctgtaggaaa gttaactacg gatgtacatt atggaactga
     7561 cgactcgcac tttaccttcg cggaaacata ttgcgctggt ggcacacgat cactgcaaac
     7621 aaatgctgat gagctgggtg gaacggcatc aaccgttact ggaacaacac gtactgtatg
     7681 caacaggcac taccggtaac ttaatttccc gcgcgaccgg catgaacgtc aacgcgatgt
     7741 tgagtggccc aatggggggt gaccagcagg ttggcgcatt gatctcagaa gggaaaattg
     7801 atgtattgat tttcttctgg gacccactaa atgccgtgcc gcacgatcct gacgtgaaag
     7861 ccttgctgcg tctggcaacg gtatggaaca ttccggtcgc caccaacgtg gcaacggcag
     7921 acttcattat ccagtcaccg cattttaacg acgcggtcga tattctgatc cccgattatc
     7981 agcgttatct cgcagaccgt ctgaagtaat attgcacagg tggcaaacgc cacctgtttc
     8041 ttacggtttt ctcgccaccg gcacatccag atttttcagt atatccacaa agggagaggg
     8101 attctctttg ttaaacagtg cccatacccg atggcgtgcc cgggtcagcg ccacgtacat
     8161 taaccgccgt tcttcagcgt ccgggaaatc ctcaaccggt ggcagtagcg cctcttccat
     8221 aatcgactcc cgcgccgcag ccggaaaacc atcactcccc tcctgcaagc caacgatgat
     8281 gacgtaatcc gcctgttgcc ctttgctggc atgaatggtc ataaagtcga tttgcaactt
     8341 cggccagcgt gttgctgctt tttccaggct ggcaggcctc atgtgatggt aacgcgccag
     8401 gatcagaatg cgctcttccg gtttggcata accagagagt ttatccagca aagcgtcaag
     8461 ttgaccctca tccaataacg tgacggcttt tttgtctcca ttggttaagc tgtttagcgg
     8521 ctttttaagc tggcctgggt tctgctgaat aaacctgttt gccacctcac cgatacgact
     8581 gttaaaacgg taggtcgtgt ctaaatcgca gcgatcacct tcaccaaagt tttcatggaa
     8641 agcggtggtg agcgacattt gcgcaccgct gaagcgataa atcgcctgcc agtcatcacc
     8701 aacggcgaac aacgtcgtct gactgttttg cttgcgtaat gccgctaaca acgctgcgcg
     8761 ctgcggcgag atatcctgaa attcatcaac cagaatatgc ttccacgggc tgataaagcg
     8821 acctttctcc agaatcacaa tcgcctggtg aataaggccc gaaaaatcga cagcgttttc
     8881 tgccttcagc gcacctttcc aggcttttaa taacggggcc atcaacttga tacgtttact
     8941 gaacagatcg cgaatctctt cgggtgcact ggcaatcatt tctgcctgtg caccaccgtg
     9001 catccgcatc agacttaccc agcgatcgag gcgaaaggca agacgacgct gtaatttttc
     9061 atcatcccag aaattacctt ctggcactga ccactgcatt tcttccgtca gccattgccg
     9121 ccagcccttc gcctgcgctt tcttttcgct gcattgcttg cgccactcag caataaaaag
     9181 ttcatgacgg gcagcggtat cattttccag tttgctgact atcggaactt ttttgctgcc
     9241 ctgctgaata atatgcagcg ccagcgcatg aaacgtgcgt gcggtaatgt cttcggtatg
     9301 tagtcgttct cgaatacgct cgtccatctc ttcagcggct ttgcgaccaa acgccagcaa
     9361 taaaatttgc tcaggggacg cttcaccacg cgccagcaac cagcctgcac gggccaccag
     9421 caccgacgtt tttccgcttc ctgcaccagc cagcactaac aaagaatgct cgcaattaac
     9481 gactgcccgg gcctgcgccg gattcagcgg tgaagactcg acctggcgga aaaaatccgc
     9541 atactcgata agcatggctt cggtatacgc ctgattatgc tgcaaccgag cgccttcaat
     9601 gtctttcaac caggcctgac atttacgcca cgcctcacgg cagttatcga attcttccag
     9661 tcggttaacc ggcaatggta acgccgacaa agcctggcgg atttgttgcc gcataccaga
     9721 ggtttgctca cgcgtcagcc atttattttc tccggtgcgc gtggcaatca aatccagttg
     9781 ctgacgtaaa acgccagacg caatttcgct catctcgcca ctccaccgcc gccagtgagc
     9841 atcaagatga tggtaaaaac gctgcgtctc gccccattcg gtaccgtgca gacgcaccac
     9901 tttttcgtcc ggcagtacaa actccagttc gccccatacc agaccgcgct tacagtgaat
     9961 cgccagtaat tgattgaaag gaataaggta ttcgtggcga tcgccggaga ctttaatccc
    10021 ggcattgagg atcaccgccc gatcgtaagg gtgctgtgcc agacgttttc caagcgttgt
    10081 cgctttcagt tccatgactc tgtagatcca cacgaaatta tttcttatca gtgtaaccgt
    10141 cagagaaaac gtgctccagc ccaaaaaaac gttacaattg ccgcccatta ctgaaaacca
    10201 cagtaaagcg aggttttatg cgtaccgttt tgaacattct gaactttgtg cttggcggat
    10261 ttgccaccac tctgggctgg ctgttggcga ctctggtcag tattgtgctg atttttacct
    10321 taccgctgac acgatcctgc tgggagatca ctaaactgtc tctggtgcct tatggcaatg
    10381 aagctattca tgtcgatgaa ctgaacccgg ctggcaaaaa tgtgctgcta aatactggcg
    10441 gtacggtatt gaatattttc tggctgattt tctttggctg gtggttatgc ctgatgcaca
    10501 ttgcaacggg catcgcacaa tgtatttcca tcattggcat tcctgtcggt attgcgaact
    10561 ttaaaattgc cgctattgca ctatggccgg tcggtcgtcg cgtggtatcg gtagaaacag
    10621 cgcaagctgc gcgcgaagcc aatgcacgtc gtcgtttcca ataatccact aatatggcct
    10681 ttatgctaag tcctttgctc aaacgctata cttggaacag cgcctggctg tattacgcgc
    10741 gtatttttat tgcgctttgt ggaaccacag cgtttccgtg gtggctgggt gatgtaaaac
    10801 tgacgattcc gctgacgctt gggatggtgg cagcggcgct gaccgatctc gatgaccgac
    10861 tggcgggacg tttgcgtaac ctcatcatta cgctgttctg cttttttatc gcctcggcct
    10921 cagtagaatt gctgtttccc tggccctggc tatttgcgat tggcttaacg ctctctacca
    10981 gcggcttcat tttgctcggc ggtctgggtc aacgctatgc aacaattgcc ttcggtgcat
    11041 tgctgatcgc catttacact atgttgggaa catcactgta tgagcactgg tatcagcagc
    11101 cgatgtatct tctggccggt gccgtctggt acaacgtcct gacacttatt ggtcatctgc
    11161 tgttcccggt ccgcccgctg caggacaacc tggcgcgttg ctatgaacaa ctggcgcgtt
    11221 atcttgagct caagtcgcgc atgtttgatc ctgatattga agatgaaagc caggcaccgc
    11281 tgtacgattt ggctctcgcc aacggtcagc tgatggcgac attgaatcag acgaaactct
    11341 cgctgctgac ccgcttacgt ggcgatcgtg gtcaacgggg aacgcgtcgc acactgcatt
    11401 attactttgt cgcacaggat attcacgagc gtgccagctc ttctcatatt caatatcaaa
    11461 cattgcgtga acattttcgc cacagcgacg tgctgttccg ttttcagcgg ctgatgtcga
    11521 tgcagggcca ggcgtgccag caactgtcac gctgtatttt gctgcgtcag ccttatcaac
    11581 atgatccgca ttttgagcgc gcttttacgc atattgatgc tgcgctggag cggatgcgcg
    11641 ataacggcgc tcctgctgat ttactcaaaa cactgggatt tttactgaac aatttacgtg
    11701 ccattgatgc ccaactggca acaattgaat cagaacaggc ccaggcactg ccccataata
    11761 atgacgaaaa tgagctcgct gatgacagcc cgcacggatt gagtgatatc tggctgcgtc
    11821 ttagccgtca cttcacgccg gaatccgccc tcttccgtca tgcggtaaga atgtcgctgg
    11881 tgttgtgctt cggctacgcc atcattcaga taaccggaat gcatcacggg tattggatct
    11941 tgctgacaag tttgtttgtc tgccagccaa actataacgc cacgcgccac cgcctgaagt
    12001 taaggattat tggtacgctg gtaggtatcg caattggtat tcctgtgctg tggtttgtgc
    12061 catcactgga agggcagctg gtgctgctgg ttattaccgg cgtgctcttt tttgccttcc
    12121 gtaacgtgca atacgcccat gcaacgatgt tcatcacact tttggtgcta ctgtgtttca
    12181 acttgctggg tgaaggtttt gaagtggcgt tacctcgcgt aatcgatacg ctgattggtt
    12241 gtgccattgc gtgggcggca gtgagctaca tctggcctga ctggaagttt cgcaatctgc
    12301 cgcgaatgct cgaacgcgcc acagaggcca actgccggta tctcgatgcc atactggaac
    12361 aataccatca ggggcgtgat aaccgtctgg cgtatcgtat tgcccgccgc gatgcacaca
    12421 accgtgatgc tgaactggcg tcggtggtat caaatatgtc cagcgaaccg aacgttaccc
    12481 cgcaaattcg cgaagccgcg tttcggttgc tgtgccttaa ccatacgttt accagctata
    12541 tctcagccct cggtgctcac cgggagcagt taactaatcc tgaaattctg gcgtttcttg
    12601 atgacgcagt ttgctatgtt gatgacgcgc tacatcatca acctgctgat gaagaacgcg
    12661 tcaatcaggc attagctggc ctgaaacagc ggatgcagca acttgaacca cgggcagaca
    12721 gcaaagaacc tctggtcgta caacaagttg ggttattgat tgcattactg ccagagattg
    12781 gtcgtctgca acgccagatt actcaagttc cgcaggaaac tcctgttttg gcgtaagaga
    12841 gtcagcccat tctgcaagct cctggcggcg tgccaccggg agcgcagctt catgaatacc
    12901 gataatggcc ccttcaagca taaacagaat cttttctgtc accagactgt tttgctgccg
    12961 cagtcgcaac caacacattt ttgccccaag tatacgtaac gcccgtacat ccttaatccc
    13021 cacctccccg agaatcgctt ccagatgaaa agacatattg ggcaaatctt tcagtctttc
    13081 ccgggtattg cgcgtgcttt tctctttcag cgctgcatcg agagaatatt tcgacagacg
    13141 caccagcttc agttgatttc gccatagact ttcatcaact cgatagtaat tgagggtaac
    13201 ggatcggcca cactttttat atgtcagcca gacaggcgga tgttttacac agtactgtgc
    13261 actttgctca caagcccgaa gatacaactc accatcagaa accatcgcaa acaccgtgtc
    13321 gtcaacggtc aggctgtaac taccaaacaa tgatcggtat tcaattgtgc ccaacgttgc
    13381 caggtattct tgtgatttat agatccgctt ataggagagg cttttcataa aattcctttt
    13441 aaaatcataa cataaaagaa tgattcacat taacggatcc gttaactacg aaaataggca
    13501 acttattctt aaggggcaag attaatttat gttttcccgc caccaacgac aaaatttgcg
    13561 aggctctttc cgaaaatagg gttgatcttt gtcgtcactg gatgtactgt atattcatac
    13621 agcaacccac aggggctgga ttgattatgt acacttcagg ctatgcacat cgttcttcgt
    13681 cgttctcatc cgcagcaagt aaaattgcgc gtgtctctac ggaaaacact acagccgggc
    13741 ttatcagtga agttgtctat cgcgaagatc agcccatgat gacgcaactt ctactgttgc
    13801 cattgttaca gcaactcggt cagcaatcgc gct
//
LOCUS       DAZFFE010000116        13824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFFE010000116 DAZFFE010000000
VERSION     DAZFFE010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.116"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(47..664)
                     /gene="yncG"
                     /locus_tag="ACHJ69_003930"
                     /pseudo
     CDS             complement(47..664)
                     /gene="yncG"
                     /locus_tag="ACHJ69_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            931..2430
                     /gene="ansP"
                     /locus_tag="ACHJ69_003931"
     CDS             931..2430
                     /gene="ansP"
                     /locus_tag="ACHJ69_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO5016816.1"
                     /translation="MSKHNTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETSS"
     gene            complement(2543..3604)
                     /gene="yncE"
                     /locus_tag="ACHJ69_003932"
     CDS             complement(2543..3604)
                     /gene="yncE"
                     /locus_tag="ACHJ69_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO5016817.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTNNQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3846..5948
                     /gene="pqqU"
                     /locus_tag="ACHJ69_003933"
     CDS             3846..5948
                     /gene="pqqU"
                     /locus_tag="ACHJ69_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO5016818.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAASQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNRGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLTDKLSLDAGVRYSSVWFDSNDHYVTPGNSDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYIPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNFTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGINIAWRFE"
     gene            complement(6031..6180)
                     /locus_tag="ACHJ69_003934"
                     /pseudo
     CDS             complement(6031..6180)
                     /locus_tag="ACHJ69_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            6429..6947
                     /gene="mddA"
                     /locus_tag="ACHJ69_003935"
     CDS             6429..6947
                     /gene="mddA"
                     /locus_tag="ACHJ69_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO5016819.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            6944..7393
                     /gene="ydcZ"
                     /locus_tag="ACHJ69_003936"
     CDS             6944..7393
                     /gene="ydcZ"
                     /locus_tag="ACHJ69_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO5016820.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLISGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRSF"
     gene            complement(7394..7627)
                     /gene="ydcY"
                     /locus_tag="ACHJ69_003937"
     CDS             complement(7394..7627)
                     /gene="ydcY"
                     /locus_tag="ACHJ69_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO5016821.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(7713..7886)
                     /gene="ortT"
                     /locus_tag="ACHJ69_003938"
     CDS             complement(7713..7886)
                     /gene="ortT"
                     /locus_tag="ACHJ69_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO5016822.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            8081..8176
                     /gene="yncL"
                     /locus_tag="ACHJ69_003939"
     CDS             8081..8176
                     /gene="yncL"
                     /locus_tag="ACHJ69_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO5016823.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(8273..9697)
                     /gene="patD"
                     /locus_tag="ACHJ69_003940"
     CDS             complement(8273..9697)
                     /gene="patD"
                     /locus_tag="ACHJ69_003940"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO5016824.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVNAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFADAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGSPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(9719..10513)
                     /gene="ydcV"
                     /locus_tag="ACHJ69_003941"
     CDS             complement(9719..10513)
                     /gene="ydcV"
                     /locus_tag="ACHJ69_003941"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5016825.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(10503..11444)
                     /gene="ydcU"
                     /locus_tag="ACHJ69_003942"
     CDS             complement(10503..11444)
                     /gene="ydcU"
                     /locus_tag="ACHJ69_003942"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5016826.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIIVRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(11445..12458)
                     /gene="ydcT"
                     /locus_tag="ACHJ69_003943"
     CDS             complement(11445..12458)
                     /gene="ydcT"
                     /locus_tag="ACHJ69_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5016827.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGTPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGEMQANGT
                     IQAVQYQGAATRFELTLSGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVALV
                     EER"
     gene            complement(12476..13621)
                     /gene="ydcS"
                     /locus_tag="ACHJ69_003944"
     CDS             complement(12476..13621)
                     /gene="ydcS"
                     /locus_tag="ACHJ69_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO5016828.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLENPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMAKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKALDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHNLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
BASE COUNT         3339 a         3581 c         3451 g         3453 t
ORIGIN      
        1 gttaactttg ccgctgattt attaacatta atcttcggca ttattgttaa ataatggcat
       61 tcctttttag tacttcttgt aacttaggta tctgacatac agcatcagcg atcacgctga
      121 tattcgcgca ttatcctgaa accatttatg tcccggtccc catatgcgca taacgcagag
      181 atagcagtcc accaaggtta gctgctcgcc aaacgcataa ggttcagcag tgagctgcga
      241 attcagccag atataaagcg atttgcgata ttcaattaca ttcttcttta actgttcagg
      301 tgcgtcagat gcccaacgct tagggtaatc ggcgaaagtg aacgttggat agacattagc
      361 aaccagccag accaatagcc gttgaaactg ttgacgttcg gcgcgcccaa gaggtggcgc
      421 aaggtccggg cgacgatcga gaaccatcaa tgcaatcgcc gctgtctccg tcataatctc
      481 gttattttta cagcgccagt gtcggaacct gacacaacgg attcagggtt tttaataaat
      541 cgcgtgaagt tccctcatgg tcaaaaccac tgacatcaac gaactgataa ggaatatcag
      601 ccagtgttag catcagctca ctgattgtag agccccagcc gggtacgcta tacaccttaa
      661 tcatatgccc ctcacaatct tttaaagatt aagtgtagga cagcacacag gttatccagc
      721 aaaaggattt atcatttttt cgtcagttgt ataaatcccc ttcaggattg taaggggatg
      781 ttttgcatcg tttatcgata acgggtagga tgctgcgccg atagttttat acccataaag
      841 aataatggtg ataactatca tcgccaggat gaataaacat tgttcatggc aacttatatg
      901 actttttcat taaagcaatc agggagagca atgagtaaac acaacaccga cacttcagat
      961 caacacgccg cgaaacgtcg ctggcttaat gcccacgaag aggggtatca caaagcgatg
     1021 ggcaatcgcc aggtacagat gatcgccatt ggcggcgcga ttggtacggg tttgttttta
     1081 ggcgcggggg cgcgactgca aatggcgggg cccgcactgg cactggtcta tttgatctgc
     1141 ggcctgtttt cgttttttat tctgcgtgca ttgggtgagc tggtgttaca ccgcccttcc
     1201 agtggcagtt ttgtctctta tgcccgtgag tttttgggtg agaaggccgc ttatgttgct
     1261 ggctggatgt acttcatcaa ctgggcgatg acggggattg ttgatattac cgccgtcgct
     1321 ctgtatatgc attactgggg cgcgtttggc ggcgtgccgc agtgggtctt tgcgctcgct
     1381 gcactgacca tcgttggcac catgaatatg atcggtgtga aatggtttgc ggagatggag
     1441 ttctggtttg cgcttattaa agtgctcgcc attgtaacct ttttggtcgt gggtacagtg
     1501 ttcctcggta gtggtcagcc gctggatggc aacaccactg gctttcattt aatcaccgat
     1561 aatggcggct tcttccccca cggtttgctg cctgcgctgg tgttgattca gggcgtagtg
     1621 tttgcttttg cctccattga aatggtgggt acagcggccg gagaatgtaa agatccgcag
     1681 accatggtgc ctaaagcgat taacagtgtg atttggcgta ttggcctgtt ttacgtcggc
     1741 tccgtggtgt tgctggttat gttattgccg tggagcgcgt atcaggcggg gcaaagtcca
     1801 tttgtgacgt ttttctctaa actgggtgtg ccgtacatcg gcagcattat gaacattgtg
     1861 gtgctgaccg ctgccctctc cagcctgaat tcaggtctgt actgcaccgg gcgtattctg
     1921 cgctcgatgg cgatgggcgg ttccgcaccg agttttatgg cgaaaatgag tcgtcagcat
     1981 gtgccgtatg ccgggattct ggcgacactg gttgtgtatg tcgtcggcgt attcctcaac
     2041 tatctggtgc cgtcacgcgt atttgagatt gtgttgaact tcgcgtcgct gggaatcatc
     2101 gcttcatggg cgtttatcat cgtttgccag atgcgcctgc gtaaagcgat taaagaaggc
     2161 aaagcagcgg atgtcagttt taaactgcct ggcgcgccct tcacttcctg gctgacatta
     2221 ctgtttttac tgagtgtcct ggtgctgatg gcgttcgatt acccgaacgg gacttatacc
     2281 atcgcggcac tgccgattat cggtattctg ctggttatag gctggtttgg tgtgcgcaaa
     2341 cgcgttgctg aaattcacag cactgcgcca gtcgtcgagg aagatgaaga aaaacaggaa
     2401 attgtgttta agcctgaaac gtcgagttaa ttgcaaaatt gcccggctac gtgccgggca
     2461 aattcagact actgacgaag gtgccggatg cggcgtgata aaaatcaggc aattctttcc
     2521 gttttcaccc tcgccatatc tattacagcg caatacgaat cacatcgtcc ggctgggtag
     2581 cttcctgctg tttagtggat ttttgtttca cactgacata cagcgttttg ccatcggcag
     2641 acagcgccag gctgttcgga tgagtcggcg tatcgaacgt tttcaccact ttatagcttt
     2701 tcgcgtcaat cacactgact ttacctgcct gacgatgcgt cacgtaggct tcattacgcg
     2761 ctgggttaaa cagcacagcc agtgattccg gtgccgcaac cttcgccaga atattgccat
     2821 tacgggtatc gaccactaac acttctgccg ctttagaatc ggtgataaat gcacgctgat
     2881 tgttggtatc aaggctgatg ttgataaaga agtgctcttt gccgtcatcc agcagctttt
     2941 tacggctgag gattttattg tcggcggtgt cgatggtaat caattcgccg tcagcgttag
     3001 tggtgtaaag acgtttgcct ttgctatcca gcgccagacc ggtactcatt ttaccggtgt
     3061 tctggatggc ggttttcagt ttgatattct cgccatcaac gacccaaatc acgctctctt
     3121 taccaatacc actgatgtaa acggtgttcg tggcatcatc agctaccagc tcacgtggtt
     3181 gcaacgggcg tacctcttcc gtgcgcttac gatcatccag caccagacga cctttcactt
     3241 cgcccgtttt ggcatctatc gccgtgaccg cactatttac ggtgttacca aaccacaacg
     3301 tctgagtcgt gttattgatg gtggcaccaa acggcttgag atcgttatgg atcgcctgcg
     3361 tcacttccag agtaaccgga tcaagacgat aaaccacgcc gcctttatcc agtttgcggc
     3421 tttgcgaagt ggcgagccac agcgcgtttt cttgctggct ataagccatt tcgtaggcac
     3481 ctttacctac cgctttacgc agcatttctt ctgcggcctg cgtactgaat gatgaagcaa
     3541 caagcaatga acctaacagt aatgaaccac gcaggcgcga tgaaaacaga tgacgtaaat
     3601 gcatgacgac tccctttgat gaaccgatat ttttgctgct tcacaatgct tcgctaagcg
     3661 taatcatcgt tacgcccttg ttataaatgg gaatggtaat cattattttc tcaaatgtgg
     3721 agcatttctt accgttacga ctacaatttt ctcaattact taacgtattg tgccgtttcg
     3781 ttttccataa atttacaatt tcattaccat gacggtgttt tcccattctt aacctggctt
     3841 ttttcatgaa gattttttcc gtccgacaga ccgttttgcc cgcactgctt gtcctttccc
     3901 ccgttgtttt tgccgctgat gaacagacca tgattgtcag tgccgcatcg caggtggttt
     3961 cagaactgga taccccagca gccgtgagcg tggtggatgg cgaggagatg cgcctggcaa
     4021 caccgcgcat taacttgtct gaatcactga ccggagtgcc tggtttacag gtacaaaacc
     4081 ggcagaacta tgcgcaagat ttacagctgt cgattcgcgg atttggctcc cgctccactt
     4141 acggaattcg cggtattcgc ctgtatgtgg acggtattcc cgctaccatg cccgacggac
     4201 aagggcaaac atctaacatc gatttaagca gtgtgcaaaa tgtggaagtg ctgcgtggcc
     4261 ccttctctgc tctgtatggc aacgcgtctg gcggtgtaat gaatgtcacc acccagaccg
     4321 gacaacagcc accaaccatt gaagccagta gttactacgg cagttttggc agctggcgct
     4381 atgggctgaa agctacgggc gcaacgggcg acggcacaca acccggcgat gtcgattaca
     4441 ccgtctcaac cacacgtttt acgacccacg gctatcgtga ccatagcggc gcacagaaaa
     4501 atttagccaa tgccaaactg ggcgtacgca ttgatgacgc cagcaaatta agcctgattt
     4561 tcaatagtgt ggatatcaaa gcagatgacc caggtgggct aaccaaagca gaatggaagg
     4621 cgaatccaca acaagcgcct cgtgcagaac agtacgacac gcgaaaaacc atcaagcaaa
     4681 ctcaggctgg gttgcgctat gagcgtagcc tgagcgcgca agatgatatg agtgtgatga
     4741 tgtatgccgg agagcgagaa acgacccagt accagtcaat cccgatggct ccccaactta
     4801 acccgtcaca tgcgggcggc gtgattactc tgcaacgcca ttatcagggg atagacagcc
     4861 gctggacaca ccgtggtgaa ctgggcgttc cagtcacgtt cacaaccggc ctgaactacg
     4921 aaaacatgag tgaaaaccgc aagggttaca ataacttccg cctgaatcgc ggcgtgccgg
     4981 aatacgggca aaaaggtgag ttgcgccgcg acgaacgcaa tttgatgtgg aacgtcgatc
     5041 cctatttaca aacccagtgg caactgaccg acaaactttc gctggatgct ggcgtgcgct
     5101 acagctccgt gtggtttgat tccaatgatc attacgttac tccgggtaac agcgacgaca
     5161 gcggtgatgc cagttatcac aaatggctac ctgccggttc gttaaaatat gcaatgaccg
     5221 atgcctggaa tatctatctg gcagccgggc gaggttttga aacgccgacg attaatgagc
     5281 tgtcttatcg tgctgatgga caaagcggta tgaactttgg gttaaaaccc tccaccaacg
     5341 atacaattga gatcggcagt aaaacgcgta ttggtgatgg gctgcttagt ctcgcattgt
     5401 tccagaccga caccgatgat gaaattgttg tcgatagcag tagcggtggg cgtacgactt
     5461 acaaaaatgc cggaaagacc cgtcgtcaag gcgctgaact ggcatgggat caacgtttcg
     5521 cgggagattt tcgcgtaaaa gcgtcctgga cctggcttga tgcgacctat cgcagcaatg
     5581 tgtgcaatga acaggattgt aacggtaacc ggatgccagg gatcgcccgt aatatgggct
     5641 ttgcgtcgat aggttatata ccggaagatg gttggtatgc aggcacggaa gcgcgttata
     5701 tgggcgatat tatggcagat gatgaaaata cggccaaagc gccttcttat acactcgtcg
     5761 gcttattcac cgggtataaa tacaattacc acaattttac cgtggattta tttggtcgtg
     5821 tcgataattt attcgataaa gaatacgttg gctctgtcat tgtcaatgag tcaaacgggc
     5881 gatattacga acctgcgccc ggacgaaatt atggtgtcgg cataaatatt gcgtggcgat
     5941 ttgagtaatt atctttaagc ccggtatttg ccgggcgtat tttatttatc ggctgtattg
     6001 ttggcacaac aaagcaacgt ttttttgcat agtgaggttt tcgacagcca gagcaactgc
     6061 cattaattcc agttcgtagc ggatccgatt gatttcattt aattgacgtt ttcccacttc
     6121 cggaactgta aatgcttgcg ctggtgcgac aaagagcgca tttaccgaaa ccagacgtaa
     6181 ggaaccggcg cgccatgcgg acgcaaggac agaacccaac atcggttacg ctgcgtttgt
     6241 tgccccataa tgctctcctg tgcttttggc atgaatccat caatagtagc aggctcacag
     6301 gattggtttt gtttgcagcc gtcacatccc ctctgcaaca cgcaataatt tcatagagga
     6361 atccgttgct tcgggctgtt ttcgatagta ttgataacat catcatcatt attttatcga
     6421 ggccagacat gtccatccgt tttgcccgca aagccgactg tgctgcaatt gcggaaattt
     6481 ataaccacgc cgtgttgtat acggcggcta tctggaatga ccaaacggtg gatgcggata
     6541 accgcattgc ctggtttgaa gcgcggacca tagcaggtta tccggtgctg gtgagtgaag
     6601 aagatggcgt agtgacggga tatgcctcgt ttggcgactg gcgtagtttt gatggttttc
     6661 gccataccgt ggaacattcg gtttatgtcc atcccgatca tcaggggaaa ggtctggggc
     6721 gtaaattgtt aagccgattg attgatgaag cgcgggattg cgggaagcat gtcatggtcg
     6781 ccgggatcga atcgcaaaat caggcctcgc tgcatctgca ccagtcgctg ggatttgtcg
     6841 tcaccgcgca aatgccgcag gtaggtacta aatttgggcg ctggctggat ctgacattta
     6901 tgcagttgca actcgacgag cgcactgaac cggacgcgat cggatgaatc agtcacttac
     6961 ccttgccttt ttgattgcag ccgggattgg tctggtagtc cagaacacgc tgatggtgcg
     7021 catcacccag acctcctcta ccattctcat cgccatgttg ctgaactcac tggtggggat
     7081 tgtgctcttt gtttctattt tatggtttaa acaaggcatg gcggggtttg gcgaactggt
     7141 gtccagcgtg cgctggtgga cacttatacc tggcttgctg ggatcgtttt ttgtcttcgc
     7201 cagtatcagc ggatatcaga atgtgggagc ggcgacaact attgcagtac tggtcgccag
     7261 ccagctgatt agcggtctgg tgctggatat cttccgcagc catggcgtac cacttcgggc
     7321 gttgatcggc ccaatttgtg gcgcaatttt acttgtggtt ggcgcctggc tggtggccag
     7381 acgctcattt taatcagagg atggtaccgc ctttggttaa ctgttcgtgg cgcgcttcca
     7441 tatcttcttt atgtttgcga ccgtgatgag caatagcggt gcgcaaacgc tgctgctggg
     7501 tgtagcgatc ttcccgactt aactccgcgt catcgctcag ggcgattaat aattcgttca
     7561 tatgggcaat gacgctctct tcaatcgcgg catccacgcg cgcgatgact tcatccagat
     7621 gcgacatact ttctccttag ccacttgtga aatttatcga ttcatccaaa tgatataagt
     7681 ctgcgtggtg ccgtcaagcc agtgcataag atttaaaaca gagaaaacaa cagcgccacc
     7741 gggaaactca tcggccatgt tgcccccacc agaaaggcgc ttaagaaacg gatgcgtttc
     7801 ttatcgtgag aaagaaacca ggtgataaga aatgcgattg ctgccataac cgcataaaaa
     7861 acaagcatat gttgatagag agacatgtac gggcgtccag ccagatgaat aaaacgcgcg
     7921 caaatatgcg tcattttttg acgtgcatca agttttttca ccagttagag aaaaaatcat
     7981 caaacagcaa cttgccctta tctatcttca gaatgagtat tattgcggat tttcttaacc
     8041 cgtactatac acataggggc aatgataaaa ggtggcaaaa atgaatgttt ccagtagaac
     8101 tgtagtactg ataaatgtct ttgctgctgt tggtttgttt actcttatct ctatgagatt
     8161 tggctggttt atttgatgtt gtgattttgc acgcagcacg tactgtcaga gaaatcagtg
     8221 ctctgacagt acgcaagatc atctcatact cttacgatct gcaatattat ccttaatgtt
     8281 taaccatgac gtggcggacg acggtgtaat cctccagccc ataaagtgac atatccttgc
     8341 cgtaaccaga aagtttctgc ccaccgtgcg gcatttcact taccagcatg aaatgggtat
     8401 tgacccaggt acaaccatat tgcagccgcg cgctgacgcg atgcgccctg cccacatcct
     8461 tcgtccatac cgaagatgca agtccgtact gactgtcatt cgcccagttc accacctgtt
     8521 cttcgttgtc gaagagcgta acactcacta ccggaccaaa tacctctttt tgcacgatgg
     8581 catcgtcctg taatgcgcca gccagcagcg tcggcgcata gtaatagcca ttacccttgc
     8641 gcttttcacc gccagtgatc actttgatgt gccctgtcgc tttcgcctct tctaccgcct
     8701 tgctgacgcg ttcgagatgc gccagcgagc ttaaaggtcc aagctccgta gactcgtcat
     8761 ccggcgaacc cgattttaac gttgccaccg cagcaccgag tttttccacc agcgtatcgt
     8821 aaatgccttt ttgcgcgtag atccgacaag ccgcagtaca atcctgtccg gcattgtaat
     8881 agccaaaagt acgtacacct tcgaccactg cttcaatatc cgcatcatca aaaacaatca
     8941 ctggcgcttt gccaccaagt tccatatgag tacgcttaat ggacggcgcg gtatggctga
     9001 tgatgtgttc cccagtggcg atagaacccg tcagcgacac catccgcact ttgggatgtc
     9061 cggtcagcgg atctcccacc gttttgcctc tgccaaacag cacgttaatc acgccagccg
     9121 ggaagatatc tttcgccagc tctgccaact tcaacgcggt cagcggggta atttctgatg
     9181 gtttaagcac tacgcagttc cctgccgcca gcgccggagc cagtttccac gcagccatca
     9241 tcagcggata attccacggt gcgatagaag ccacgacccc caacggatcg cgacggatca
     9301 tcgaagtatg accttcaaga tattcacctg ccgccagacc attcaggcag cgcgccgcat
     9361 cggcgaaaaa gcgaaagaca tcgacaatcg ccggaatttc atcattgaac gcactatgca
     9421 gcggtttgcc acaattacgg gactccagtt cggcaaaaac ctgaccattt tcttcgataa
     9481 catcagccag tttcagcagg cattccgcac gcgcttttgg cgtggtttgc ccccattcgg
     9541 caaatgctgc atctgccgcg cgaacagcag cattgacctg ctctgcggat gcctcggcaa
     9601 tctccagtaa aacgtccccc gttgccggat tatagacagg ctgtttttcc ccttcgccgc
     9661 taaccagttc tccgttaatc agtaacttat gttgcatagc atattcctgt atcagtggtt
     9721 attgaccatt gtcgccttcg cgggttagcc accaggcccc caggatcggc aaggttgtta
     9781 ccaacataac cagcagtgcc accacgttgg ttaccggtac atcacgcggt cgcccaagct
     9841 gattgagcaa ccacaacggt aacgttcgtt catgacctgc cgtaaaagtc gtcacgatga
     9901 tttcatcgaa cgacaaggca aacgccagca ttcctccagc cagtaacgcc gaactgagat
     9961 tcggcaacac tacgtagcgg aaggtttgcc agccattggc cccaagatcc attgacgcct
    10021 caaccagact ccacgaggtg cggcgaaaac gggcgatgac attgttaaac accaccacta
    10081 cgcaaaaagt cgcatgaccg accacgatgg tgaaaaatcc cggctccaga ttgatggttt
    10141 taaaggcggt taatagcgcc agaccagtga caatgcccgg cagcgcaatg ggcagcaaca
    10201 gtaacagcga aatggcgttt ttgccgaaaa agtctcgtcg ccacagcgcg gcagcggcca
    10261 gcgtccctaa caccagcgcg attaatgtcg ccagcgccgc cactttaagt gacagtgtca
    10321 cggcatcaag aatatcacta cgctgtgctg ccacgctaaa ccagcgcagc gtcaggcctt
    10381 gcggtggaaa actaaacgcc gcatcttcag tgttaaacgc ataggcagcg ataatcagga
    10441 tgggaaaatg taggaaaacc acgccgcccc aggccgccag tttgaggaaa aacggtgcgc
    10501 gttcagagtg catcgaacgc tcccagacgt ttcacgaacg ccaggtaaag tgcgatgaga
    10561 acaatcggca ccagggtgaa tgccgccgcc atcggcatat tgccaatcgc cccctgctgg
    10621 gaataaacca tattgccgat aaaatatcct ggagggccaa ccagctgcgg gacaataaaa
    10681 tcgcccagcg tgagtgagaa ggtaaagata gagccagcgg caatacccgg gattgccagc
    10741 ggcaacacca catagcgaaa ggtttgtcgt ggacgtgcgc caagatcagc cgacgcctgc
    10801 aacaatgacg gcggcaaacg ctcaagcgcc gcctgcacgg gcaggatcat gaacggcagc
    10861 cagatataga gaaacaccag aaagcgcccc agcccggaag ttgacagcgt attacctccc
    10921 accgccggta gcgtgaggaa cgcagtcagc agtggttcca gcccaagatg ttgtaaaaac
    10981 cactgagcca cgccatcttt tgccagcaat aacgtccagg cataggcttt aacaatgtag
    11041 ctcgcccaca tcggcaacat taccgcaata taaaaaaacg ctttcatttt tccgctggta
    11101 tagcgcgcca tataccacgc cattggaaaa gccagaatgg cgctggcgat agtgactgcc
    11161 acagccatgg tcagcgtgcg gacaatgatg tcgtaattcg ccggattaaa cagcgcacgg
    11221 atattcgcca gcgtcagttc cggcgttacc gacatggtga aatcgtcaaa ggtataaaag
    11281 ccctgccata acagcgtcag cagcgagccg aaatagacaa tgccaaacca cattagcggg
    11341 ccaagcagca gcaaaaacag ccccagcccc ggattacgcc agaagaaacc ggacacctta
    11401 cccagacctg gacgtgaagg tgattgcaat acgttcatcg ccattcacct ctcctcaacc
    11461 agcgccacca tcacatcacg cgaccaggaa accatcacct gttgtccggg cgtgagcgtg
    11521 gcaggcagtt cttcgcctgt catattggcc tgactcacca gcagtttttc accgccgctc
    11581 agtgtcagtt caaaacgggt cgctgcgccc tgatattgca ccgcctggat cgtgccatta
    11641 gcctgcattt cgccaggggt gttgaggcgg atatgttccg gtcgtagtgc gaagcttccc
    11701 gtcatgccac aaagtttctc tgccatcagt ccatcaaaaa cattcgatgt cccaacgaat
    11761 cccgcaacaa acggcgtgcg cgggcgcata tagagatcgc gcggagaatc gacctgttca
    11821 atgcgtccat tattgaaaac cgccacccga tcggacatcg ataacgcttc gccctgatcg
    11881 tgggtaacga agataaaagt gataccgaga gactgttgca gctttttcag ctcaagctgc
    11941 atttgctctc gcaatttgag atccagcgcg ccgagcggtt catccagcaa caacacacgc
    12001 ggttcgttca ccagtgctct ggcgatggcg actcgctggc gctgaccacc agaaagttgt
    12061 gacggtttac gttgctgtac aaaccccaac gccactttct caagcgcctc ttgcgccatt
    12121 gcatgccgct gctttttatt cacgcctttg accatcagcc cgtaggctac gttgtcaaga
    12181 atcgacatat gcggaaatag cgcgtagtcc tggaagacag tattcacgtc ccgctcccac
    12241 ggtggcagat tgctggcggg cgtaccaaaa atagagatag ccccgccgga aagctgttcg
    12301 aatccagcaa tcaggcgcag gcaagtggtt ttgccggagc cggacggccc cagcatagag
    12361 aagaactcac catcttttat cgcaatactg acgccatcta ctgcgcgcac gtcaccgtac
    12421 aaccgtgaga cgttgtcaaa ctccactgcg tacgtcataa aacaccccag cgaagttagc
    12481 gaccgcccat aatggcaatg taatcctgcg tccagcgact gtagggaaca aacttgcccc
    12541 cttctgctat aggcgttttc cagaaggcga ttttatcgaa atagttaaaa ccgtttgttt
    12601 cacaaccttt ctcgcctaat aacggactgg ctttacaccc ttgcggcact actggtaacg
    12661 agccaaacca ggccgccaca tcgccctgca cttttggggt taatgaccag ttcatccatt
    12721 tgtaggcgca aaccggatgt ttcgcttcgc tatgtagcat ggtggtgtca gcccaaccgg
    12781 taacgccctc cttcgggaaa acggtagcga caggctggcc ttcggctttc agggcgttgg
    12841 cctgataggg ccatgcactg gaagcaacca caccctcgtt tttgaaatcg ctcatttgca
    12901 cggtagtgtc atgccagtag cgatggatca aattatgttg atcgcgcagc actttcagca
    12961 ccgcctgata ctgttcttcg gtgagttgat acggatcact gatgcccaac tgtggctgcg
    13021 tggctttgac gaacaacgcg gcatccgcaa tgtagatagg gccatcataa gcctgtaccc
    13081 ggcctttatt gctcttgccg tccggcagat tctgctcaac aaaaaccact tgccagctat
    13141 ccggcggcgt cgggaaggtt ttagtgttgt acatcagcag gttcggcccc cattggtaag
    13201 gtgtgccgta aactttgcca ccaacgttaa accagtcgcc tttaaccacg cgcggatcga
    13261 gcgctttcca gttgggaatc aaggcggtat taattggctg cacgcgtttg cccataatca
    13321 aacgcagcga ggcatcgccg gatgccgtaa ccagatcgta accccctttg gccatcagac
    13381 tgaccatttc atcggaagtc gcggcggttt taacattcac cgcgcagcct gtctcttttt
    13441 cgaattgcgt tacccagtcg tactgtttat cagtttgtcc gcgttcgatg tatcccggcc
    13501 aggcgataat atccagtcgc ccttccggat tctctaaatt ggtaggcggt tcggcggcgt
    13561 gagcggtcat tattgtcatg ctgagcgcac acaggctgct gcgggcaaat gtcttgctca
    13621 taaggtctta ctcctgtcgt aattaattgt tcagccgccg tgccgtaaaa atatctcctt
    13681 gtttaaccat agacggcggc ttgcaggcac accttcggcc ctgagaaaat ttaatcaggt
    13741 tgtgagctac cgcgcattat gccgtctgta aggttatctt ctggagtata gacaaggagt
    13801 tagaatatat gactgctatt cgaa
//
LOCUS       DAZFFE010000117        13524 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFFE010000117 DAZFFE010000000
VERSION     DAZFFE010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.117"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            250..1359
                     /gene="livK"
                     /locus_tag="ACHJ69_003945"
     CDS             250..1359
                     /gene="livK"
                     /locus_tag="ACHJ69_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO5016829.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINTKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVAFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPTNQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1407..2333
                     /gene="livH"
                     /locus_tag="ACHJ69_003946"
     CDS             1407..2333
                     /gene="livH"
                     /locus_tag="ACHJ69_003946"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO5016830.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMISSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2330..3607
                     /gene="livM"
                     /locus_tag="ACHJ69_003947"
     CDS             2330..3607
                     /gene="livM"
                     /locus_tag="ACHJ69_003947"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO5016831.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTAKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWSCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3604..4371
                     /gene="livG"
                     /locus_tag="ACHJ69_003948"
     CDS             3604..4371
                     /gene="livG"
                     /locus_tag="ACHJ69_003948"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO5016832.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4373..5086
                     /gene="livF"
                     /locus_tag="ACHJ69_003949"
     CDS             4373..5086
                     /gene="livF"
                     /locus_tag="ACHJ69_003949"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO5016833.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5483..6799
                     /gene="ugpB"
                     /locus_tag="ACHJ69_003950"
     CDS             5483..6799
                     /gene="ugpB"
                     /locus_tag="ACHJ69_003950"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO5016834.1"
                     /translation="MKPLRYTASALALGLALMANAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADIATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6897..7784
                     /gene="ugpA"
                     /locus_tag="ACHJ69_003951"
     CDS             6897..7784
                     /gene="ugpA"
                     /locus_tag="ACHJ69_003951"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO5016835.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDAFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7781..8626
                     /gene="ugpE"
                     /locus_tag="ACHJ69_003952"
     CDS             7781..8626
                     /gene="ugpE"
                     /locus_tag="ACHJ69_003952"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO5016836.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQEVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLISPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8628..9698
                     /gene="ugpC"
                     /locus_tag="ACHJ69_003953"
     CDS             8628..9698
                     /gene="ugpC"
                     /locus_tag="ACHJ69_003953"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO5016837.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMVWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLAGRVNNEGTHFELDGGITLLLNGGYRQYAGRKM
                     TLGIRPEHIALSSRAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            9695..10438
                     /gene="ugpQ"
                     /locus_tag="ACHJ69_003954"
     CDS             9695..10438
                     /gene="ugpQ"
                     /locus_tag="ACHJ69_003954"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO5016838.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGARYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLCVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKTVALAARELWTGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10425..10865)
                     /gene="yhhA"
                     /locus_tag="ACHJ69_003955"
     CDS             complement(10425..10865)
                     /gene="yhhA"
                     /locus_tag="ACHJ69_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO5016839.1"
                     /translation="MKRLLLLTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKEMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10985..12718
                     /gene="ggt"
                     /locus_tag="ACHJ69_003956"
     CDS             10985..12718
                     /gene="ggt"
                     /locus_tag="ACHJ69_003956"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO5016840.1"
                     /translation="MIKPTFLRRAAIAALLTGSCFSTVAAPPVSYGVEEDVFHPVRAK
                     QGMVASVDATATQVGVDILKEGGNAVDAAVAVGYTLAVTHPQAGNLGGGGFMLIRSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLIRKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMQKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQVLTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTIKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            complement(12756..13040)
                     /gene="yrhB"
                     /locus_tag="ACHJ69_003957"
     CDS             complement(12756..13040)
                     /gene="yrhB"
                     /locus_tag="ACHJ69_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFO5016841.1"
                     /translation="MITYHDAFAKANNYLDDANLPVVITLHGRFSQGWYFCFEAREFF
                     ETGDEAARLAGNAPFIVDKGSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
     gene            complement(13240..13395)
                     /locus_tag="ACHJ69_003958"
                     /pseudo
     CDS             complement(13240..13395)
                     /locus_tag="ACHJ69_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3081 a         3370 c         3787 g         3286 t
ORIGIN      
        1 tattctgcca tttgtaccgt tattttatgc tgacaacggc gcttttttct gtttatctat
       61 caataaattc agaatattat ctgttcttaa tcgactgaaa aatagggatt ttaatcgcta
      121 tcatcacaaa atactgcgct aaacccttaa tcagacaggc aaaaacagtg cagtataaaa
      181 aaagaacagt ctgatttgtt aacacataaa aacaaagcaa cacaacatca cgaatgggga
      241 tttttgacta tgaaacggaa tgcgaaaact atcatcgcag ggatgattgc actggcaatt
      301 tcacacaccg ctatggctga cgatattaaa gtcgccgttg tcggcgcgat gtccggcccg
      361 attgcccagt ggggcgatat ggaatttaac ggcgcgcgtc aggcaattaa agacattaat
      421 accaaagggg gaattaaggg cgataaactg gttggcgtgg aatatgacga cgcctgcgac
      481 ccgaaacaag ccgttgcggt cgccaacaaa atcgttaacg acggcattaa atatgttatt
      541 ggtcatctgt gttcctcctc tacccaacct gcatcagata tctatgaaga cgaaggtatt
      601 ctgatgatct cgccgggagc gaccaatccg gagctgaccc aacgcggtta tcaatacatc
      661 atgcgtactg ccgggctgga ctcttcccag gggccaacgg cggcaaaata cattcttgag
      721 acggtgaagc cccagcgcat cgccatcatt cacgacaaac aacagtatgg cgaagggctg
      781 gcacgttcgg tgcaggacgg gctgaaagcg gctaacgcca acgtcgcctt cttcgacggc
      841 attaccgctg gagagaaaga tttctccgcg ctgatcgccc gcctgaaaaa agaaaacatc
      901 gacttcgttt actacggcgg ttactacccg gaaatggggc agatgctgcg ccaggcccgt
      961 tccgttggcc tgaaaaccca gtttatgggg ccggaaggtg tcggtaatgc gtcactgtcg
     1021 aacattgccg gtgatgccgc tgaaggcatg ttggtcacca tgccaaaacg ctatgaccag
     1081 gatccgacaa accagggcat cgttgatgcg ctgaaagccg acaagaaaga tccgtccggg
     1141 ccctatgtct ggatcaccta cgcggcggtg caatctctgg caactgccct tgaacgtacc
     1201 ggcagcgatg agccgctggc gctggtgaaa gatttaaaag ctaacggtgc aaacaccgta
     1261 attgggccgc tgaactggga tgaaaaaggc gatcttaagg gattcgattt tggtgtcttc
     1321 cagtggcacg ccgacggttc atccacggca gccaagtgat catcctaccg tccgtgaaaa
     1381 acgggcgggt ttagaaaggt taccttatgt ctgagcagtt tttgtatttc ttgcagcaga
     1441 tgtttaacgg cgtcacgctg ggcagtacct acgcgctgat agccatcggc tacaccatgg
     1501 tttacggcat tatcggcatg attaacttcg cccacggcga ggtttacatg attagtagct
     1561 atgtctcatt tatgatcatc gccgcactga tgatgatggg tattgatacc ggctggctgc
     1621 tggtggcggc gggattcgtc ggcgcaatcg tcattgccag cgcctacggc tggagtatcg
     1681 aacgggtagc ctatcgcccg gtgcgtaact ctaagcgcct gattgcgctt atttccgcaa
     1741 tcggcatgtc tatcttcctg caaaactacg tcagcctgac cgaaggttcg cgcgacgtgg
     1801 cgctgcccag cctgtttaac ggtcagtggg tagtgggtca tagcgaaaac ttctctgcct
     1861 ctatcactac catgcaggcg gttatctgga ttgttacttt cctcgccatg ctggcactga
     1921 cgattttcat tcgctattcc cgtatggggc gagcctgccg tgcctgcgcg gaagatctga
     1981 aaatggcgag cctgcttggc atcaacaccg accgggtgat tgcgctgacc tttgtgattg
     2041 gcgcggcgat ggcggcagtg gcgggcgtgc tgctcggtca gttctacggc gtaattaacc
     2101 cctacatcgg ctttatggcc gggatgaaag cctttaccgc tgcggtgctc ggtgggattg
     2161 gcagcattcc gggggcgatg attggcgggc tgatcctggg gattgcggag gcgctctctt
     2221 ctgcctatct gagtacggaa tataaagatg tggtctcatt cgccctgctg atcctggtgc
     2281 tgctggtgat gccgaccggt attctgggtc gcccggaggt agagaaagta tgaaaccgat
     2341 gcatattgca atggcgctgc tctctgccgc tatgttcttt gtgctggcgg gcgtctttat
     2401 gggcgtgcaa ctggagctgg atggcaccaa actggtggtc gacacggctt cagacatccg
     2461 ttggcagtgg gtgtttatcg gcactgcggt ggtctttttc ttccagcttt tgcgacctgc
     2521 tttccagaaa gggctaaaaa gcgtttccgg gccgaagttt attctgcccg cgattgatgg
     2581 ttccacggcg aagcagaaac tgttcctcgt ggcgctgctg gtgcttgcgg tggcgtggcc
     2641 gtttatggtg tcgcgtggga cggtggatat cgccactctg accatgatct acattattct
     2701 cggtctcggg ctgaacgtgg ttgttggtct ttctggtctg ctggtgctgg gatacggcgg
     2761 cttttatgcc atcggcgctt acacttttgc gctgctcaat cactattacg gtctgggctt
     2821 ctggagctgt ctgccgattg ccgggttgat ggcggcggcg gcgggcttcc tgctcggctt
     2881 cccggtgttg cgtctgcgcg gtgactatct ggcgatcgtt accctcggtt tcggcgaaat
     2941 tgtgcgcata ttgctgctca ataacaccga aattaccggc ggaccgaacg gtatcagtca
     3001 gattccgaaa ccgacgctct tcggactcga gttcagccgt accgcccgtg aaggcggctg
     3061 ggacacgttc agtaatttct ttggcctgaa atacgatccc tccgatcgcg tcatcttcct
     3121 ctacctggtg gcgttgctgc tggtggtgtt aagcctgttt gtcattaacc gcctgctgcg
     3181 gatgccgctg gggcgtgcgt gggaagcgtt gcgtgaagat gaaatcgctt gtcgttcgct
     3241 gggcttaagc ccgcgtcgta tcaagctgac tgcctttacc atcagcgctg cgtttgccgg
     3301 ttttgctgga acgttgtttg ctgcgcgcca gggctttgtc agccctgaat ccttcacttt
     3361 tgccgaatcg gcgtttgtgc tggcgatagt ggtgctcggc ggtatgggct cgcaatttgc
     3421 agtgattctg gcggctgttt tgctggtggt atcgcgcgag ttgatgcgtg atttcaacga
     3481 atacagcatg ttaatgctcg gtggtttgat ggtgctgatg atgatctggc gtccgcaggg
     3541 cttgctgccc atgacgcgcc cgcaactcaa gctgaaaaac ggcgcagcaa aaggagagca
     3601 ggcatgagtc agccattatt atctgttaac ggcctgatga tgcgcttcgg cggcctgctg
     3661 gcggtgaaca acgtcaatct tgaactgtac ccgcaggaga tcgtctcgtt aatcggccct
     3721 aacggtgccg gaaaaaccac ggtcttcaac tgcctgaccg gattctataa acccaccggc
     3781 ggcaccattt tactgcgcga tcagcatctg gaaggtttac cggggcagca aattgcccgc
     3841 atgggcgtgg tgcgcacctt ccagcatgta cgtctgttcc gtgaaatgac ggtgattgaa
     3901 aacctgctgg tggcacagca tcagcaactg aaaactgggc tgttctccgg cctgttgaaa
     3961 accccctctt tccgtcgcgc ccagagcgaa gcgctcgacc gcgccgctac ctggcttgag
     4021 cgcattggtt tgctggaaca tgccaaccgt caggcgagta acctggccta tggtgaccag
     4081 cgtcgtctgg agattgcccg ctgtatggta acgcagccgg agattttaat gctcgacgaa
     4141 ccggcagcag gtcttaaccc gaaagagacg aaagagctgg atgagctgat tgccgaactg
     4201 cgtaaccatc acaacaccac tatcttgttg attgaacacg atatgaagct ggtgatgggg
     4261 atttcagaca gaatttacgt ggtcaatcag gggacgccgc tggcaaacgg tacaccggag
     4321 cagatccgta ataacccgga cgtgatccgt gcctatttag gtgaggcata agatggaaaa
     4381 agtcatgttg tcctttgaca aagtcagcgc ccactacggc aaaatccagg cgctgcatga
     4441 ggtgagcctg catatcaatc agggcgagat tgtcaccctc attggcgcga acggggcggg
     4501 gaaaaccacc ttgctcggca cgttatgcgg cgatccgcgc gccaccagcg ggcgaattgt
     4561 gtttgatgat aaagacatta ccgactggca gacggcgaaa atcatgcgcg aagcggtagc
     4621 gattgtcccg gaaggacgtc gcgtcttctc gcggatgacg gtggaagaga acctggcgat
     4681 gggcggcttt tttgccgaac gcgaccagtt ccaggagcgc ataaagtggg tctatgaact
     4741 gtttccacgt ctgcatgagc gccgtgttca gcgagcgggc actatgtctg gcggtgaaca
     4801 gcagatgctg gcgattggtc gcgcgctgat gagcaacccg cgtttgttgt tgcttgatga
     4861 gccatcgctc gggctggcac cgattatcat ccagcaaatt ttcgacacca tcgaacaact
     4921 gcgcgagcag gggatgacca tcttccttgt cgagcagaac gccaaccagg cgctaaagct
     4981 ggcggatcgc ggctacgtgc tggaaaacgg tcatgtagtg ctctccgata ccggcgacgc
     5041 gctgctggcg aatgaagcgg tgagaagtgc gtatttaggc gggtaataac acgttgattg
     5101 ataaggagtc aaaagactcc tttgagacag gtgacaaatg taaaattgcc tgatgcgctg
     5161 cgcttatcag gcctactggg tgagttgcaa tatattgaat ttgcacgatc ttgtaggctg
     5221 gataaggcgt ttacgccgca tccggcatga aacgatgagt aatctgtaga gtttgattca
     5281 gaccttctat ttttccgctt atccgtgccc catctcccat tttccctcgc ccgcgccgtc
     5341 accgccttgt catctttctg acaccttact atcttaaaaa tgtaacaaaa tagttatttt
     5401 tctgtaattc gagcatgtca tgttactccg cgagcataaa acgcgtgaat tcgcgcattc
     5461 ggtacaacaa gagagataat caatgaaacc gttacgttat acagcttcag cactggcgct
     5521 cggactggct ttaatggcaa atgcgcaggc agtgacgacc attccgttct ggcattctat
     5581 ggaaggggaa ctgggtaaag aggtggattc tctggcccaa cgttttaacg ccgaaaaccc
     5641 ggattacaaa attgtaccga cctataaagg caactacgaa cagaatttaa gcgcggggat
     5701 tgccgcattt cgtaccggca acgctccggc tattttgcag gtttatgaag ttggcaccgc
     5761 caccatgatg gcgtcgaaag ccattaaacc ggtgtatgac gtatttaaag aggcagggat
     5821 tcaattcgat gagtcgcagt ttgtgccgac ggtttcaggc tattactccg acagcaaaac
     5881 cggccactta ctctcccagc cgttcaacag ctcgactccc gttctctatt acaacaaaga
     5941 cgccttcaag aaagcaggat tagacccgga acagccgccg aaaacctggc aggatctggc
     6001 ggactatgcc gcaaaactga aagcctccgg tatgaagtgc ggctacgcca gcggctggca
     6061 gggatggatc caactggaaa actttagcgc ctggaacggt ttgccgtttg ccagcaaaaa
     6121 caacggcttt gacggcacag acgcggtgct tgagttcaac aagccggagc aggtgaaaca
     6181 catcgccatg ctcgaagaga tgaacaagaa gggcgatttc agctacgttg ggcgtaagga
     6241 tgaatccacc gagaagttct ataacggtga ttgcgcgatg acgaccgcct cttccggttc
     6301 tcttgccaac attcgcgagt acgccaaatt taactatggc gtaggcatga tgccttacga
     6361 cgccgatgcg aaagacgcgc cgcaaaacgc cattatcggc ggagccagtc tatgggtaat
     6421 gcagggtaaa gataaagaaa cctacaccgg cgtggcgaag ttcctcgact tcctcgcaaa
     6481 gccagaaaac gctgccgagt ggcatcagaa aaccggctat ctgccaatca ccaaagcggc
     6541 gtatgacctg acccgtgagc agggctttta cgagaaaaac ccaggagcgg atattgccac
     6601 gcgtcagatg ctgaacaagc caccgttgcc gttcaccaaa gggctgcgtc tgggcaacat
     6661 gccgcagatc cgcgtgattg tggatgaaga gctggagagc gtgtggaccg gtaagaagac
     6721 accacagcag gcgctggata ctgccgttga gcgtgggaac cagttactgc gccgctttga
     6781 gaaatcgacg aagtcttaat cagtgtaatg tcggatgcgt ttcgcttatc tgacctggca
     6841 tcgcgtgtag gccggataag cgaagcgcat ccggcacagt tcaggaatta actgtaatgt
     6901 catcatcccg tccggtgttc cgctcgcgct ggctgcctta tttgctggtc gcgccgcagc
     6961 tcatcatcac cgttatcttt tttatctggc ctgcgggcga agcgttgtgg tactcgctac
     7021 aaagcgtcga tccgtttggt ttctccagcc agtttgtcgg cctggataat ttcgtcacgc
     7081 tgtttcacga cagctactat ctcgacgcct tctggacgac gataaaattc agcacctttg
     7141 tcaccgtcag cggtttgctg gtatcgctgt tcttcgcagc gctggtggag tacatcgtgc
     7201 gcggcagtcg tttctatcaa accttgatgc tgctgccgta tgccgtggct cccgccgttg
     7261 ccgccgtatt gtggatcttt ctgtttaacc ccggtcgcgg gctgatcacc cattttctcg
     7321 ctgagttcgg ctacgactgg aaccacgcgc aaaacagcgg tcaggcgatg ttcctggtgg
     7381 tgtttgcctc agtgtggaag caaatcagct acaactttct gttcttctat gccgcgctgc
     7441 aatccatccc ccgttcgttg atcgaagccg cagccattga tggggctggc ccgattcgcc
     7501 gcttctttaa gattgcgctg ccgcttatcg ctccggtgag tttcttcctg ctggtggtga
     7561 acctggtgta tgccttcttc gacaccttcc cggtgatcga cgccgccacg tccggcgggc
     7621 cagtacaggc caccacgacg ctgatttata agatttaccg tgaaggcttt accggactgg
     7681 atttggcttc gtctgccgcg cagtcggtgg tgttgatgtt cctcgtcatc gtgctgacgg
     7741 tggtgcagtt ccgctatgtt gaaagcaagg tacgttacca atgattgaga accgtccgtg
     7801 gctgacaata ttcagccata ccatgctgat cctcgggatc gcggtgatcc tcttcccgct
     7861 gtacgtggcg tttgtcgcgg cgacgctgga taaacaagaa gtctatgccg caccgatgac
     7921 gctaatcccc ggcacacatc tgctggaaaa catccacaac atctgggtaa acggggtagg
     7981 cacgaatagc gcgccgttct ggcggatgtt gcttaacagc tttgtgatgg cgttcagcat
     8041 tacgctcggc aaaattaccg tctcgatgct ctcggcattt gccattgtct ggtttcgttt
     8101 tccgctacgt aacctcttct tctggatgat ttttatcacc ctgatgctgc cggttgaagt
     8161 gcgtatcttc ccgacggtgg aagtcatcgc caacctgaaa atgctcgaca gctacgcagg
     8221 tttaacgctg ccgctgatgg cctcggcgac cgccacgttt ctgtttcgcc agttctttat
     8281 gaccctaccg gatgaactgg tggaagccgc gcggatcgac ggcgcgtcgc caatgcgttt
     8341 cttttgcgac atcgtttttc cgctgtccaa aaccaatctg gcggcgctgt ttgtgattac
     8401 ctttatttac ggctggaacc agtatttgtg gccgttgttg attattaccg acgttgatct
     8461 cggcaccacc gtggcaggga ttaaagggat gatcgccaca ggcgaaggca caacggaatg
     8521 gaactcggtg atggcggcga tgttgttaac cctgatctca ccagtagtga ttgttttagt
     8581 gatgcagcgt gccttcgtgc gcggcctggt cgatagtgag aaataagatg gcaggactga
     8641 aattacaggc agtaaccaaa agctgggatg gcaaaaccca ggtcattaaa ccgctgaccc
     8701 ttgatgtggc ggatggcgaa tttatcgtga tggtcgggcc gtctggctgc gggaaatcga
     8761 cgttattgcg aatggttgcc ggactggagc gggtaacgac gggcgatatc tggatcgacc
     8821 gcaagcgtgt caccgaaatg gagccgaaag atcgcgggat tgcgatggtg ttccagaact
     8881 acgcgctcta tccgcatatg agcgtagagg agaacatggt gtgggggctg aaaattcgcg
     8941 gcatgggcaa gcaacaaatt gccgaacgtg ttaaagaagc ggcgcgcatt ctggagctgg
     9001 acggcctgct taagcgccgc ccgcgtgagc tatccggcgg tcagcgtcag cgtgtggcga
     9061 tggggcgtgc gattgtgcgt gagccggcag tattcctgtt tgatgagccg ctctctaacc
     9121 tcgatgccaa gttgcgtgtg cagatgcgtc ttgaactgca acagctgcac cgtcgcctga
     9181 aaacgacttc actctacgtt acccacgatc aggttgaggc gatgacgctc gcccagcgag
     9241 taatggtgat gaacggcggc gtcgccgaac agattggcac gccagttgaa gtgtacgaaa
     9301 agcccgccag cctgtttgtg gcgagtttta tcggcagccc ggcgatgaac ctactggctg
     9361 gccgcgtgaa taacgaaggt acgcatttcg aactggatgg tggtattacg ctgctgctaa
     9421 acggtggcta ccgtcagtat gccgggcgta aaatgaccct cggcattcgc ccggaacata
     9481 tagcgctaag ctcgcgggca gaaggtggtg tgccgctggt gatggacacg ctggagatcc
     9541 tcggcgcaga taacctggcg cacggacgct ggggcgaaca gaagctggtg gtgcgactgg
     9601 cgcatcagga gcgcccgacg gcaggcagca cgctgtggct gcatctgccg gaaaatcagc
     9661 tgcatctttt tgatggtgaa acaggacaac gagtatgagt aactggcctt atccccgcat
     9721 cgtcgcccat cgtggtggcg gtaagctggc cccggaaaac actctggcgg caatcgacgt
     9781 cggggcaaga tacggtcata agatgatcga atttgacgca aagttatcga aagatggcga
     9841 gatcttcctg ctccatgacg acaatctcga acgtaccagc aacggttggg gcgttgcggg
     9901 cgagctgaac tggcaggatt tactgtgcgt ggatgcaggc agttggtaca gcaaagcgtt
     9961 taaaggtgag ccgctgccgt tgctttcgca ggtggcggaa cgctgccgcg aacacgggat
    10021 gatggcgaat atcgaaatca aacccaccac tggcactgga ccattaacgg gcaaaacggt
    10081 ggcgttggcg gcacgcgaac tgtggaccgg tatgacgccg ccgctgctgt catcgtttga
    10141 gattgatgct ttagaagccg cgcaacaggc agcaccggaa ctgccgcgtg gtttattgct
    10201 tgatgaatgg cgcgacgact ggcgagaatt gaccgcgcga ctgggctgcg tctctattca
    10261 tctcaatcat aagttgctcg ataaagcgcg agtgatgcag ttgaaagatg ccggattacg
    10321 gattctggtt tataccgtca ataaacccca gcgcgcggca gagttgctgc gctggggcgt
    10381 ggattgcatc tgtaccgatg cgattgacgt gattggtccg aactttacgg cccaatagtt
    10441 ttcaacggaa tgtcaggctg cggcgtgctg ggctgattca gcatgtcgcc gtttctcctc
    10501 tccggcagca tatgctgctg attcaacgaa ctatccggat tacggttgct gtttaacatc
    10561 ccgccgttgg tgttgggcag catttgctgc cgggcgggat ttcgctcccc cggctgtgac
    10621 tgcaacaccc gctgagaatt attgtttatc tggttttcta aatgctgctg ttgcagctgc
    10681 gtttgcgttt tcagttgctg gttcagcatc tctttttgct ggatttgctg cgtctgcatt
    10741 tgagtttgca tccgctgctg gctggggatc tgatatcccg gctggttagg gttgttcaga
    10801 gtattaatgg gctgtgcaaa gccgacaaac ggcaggagtg ccgtcaaaag cagaagtcgt
    10861 ttcatcgcgt atcctcctct gaagatattc tttaagttta ctcgcttccc ggcaaaacga
    10921 tgattaattc agagttatat accaggctta gctggggttg ccccttaatc tctggagaat
    10981 aacgatgata aaaccgacgt ttttacgccg ggcggccatt gctgctctgc tcacaggaag
    11041 ttgttttagt acggtagccg cgccacctgt ttcttatggt gtggaggaag atgtctttca
    11101 tccggtacgc gctaagcagg gaatggtggc gtctgtagat gctactgcca ctcaggtggg
    11161 ggtggatatc ctcaaggagg gcgggaatgc cgttgatgcc gccgtggcgg tgggctacac
    11221 gctggcagta acgcatccgc aggcagggaa tctgggcggc ggtggtttta tgctgatccg
    11281 ctcgaaaaat ggcaatacca cggctatcga tttccgcgaa atggcacccg ccaaagcgac
    11341 ccgcgatatg ttcctcgatg atcagggcaa cccggacagc aaaaaatcac tcacttcgca
    11401 tctggcttcc ggcacacccg gtacggtagc gggtttctcg ttggcgctgg ataaatacgg
    11461 caccatgccg ttgaataaag tggtgcaacc ggcgtttaaa ctggcacgcg atggttttat
    11521 cgttaacgac gcgctggctg acgatctcaa aacctacggc agtgaagtgc tgccgaacca
    11581 cgaaaacagc aaagctatct tctggaaaga gggcgaaccg ctgaaaaagg gcgacaagct
    11641 ggtgcaggcg aacctggcaa agagcctgga gatgattgcc gaaaacggcc cggatgaatt
    11701 ctacaaaggc acgatagcgg aacagattgc ccaggagatg cagaaaaacg gtggcttgat
    11761 cagaaaagaa gatttagcgg catataaagc ggtcgaacgc acaccgataa gcggcgatta
    11821 tcgcgggtat caggtttact ccatgccacc gccatcttcc ggcggtattc atatcgtaca
    11881 aatcctcaat attctggaaa acttcgatat gcagaagtac ggctttggca gcgccgacgc
    11941 gatgcaaatc atggcagaag ctgagaaata cgcctatgcc gaccgctcgg aatatcttgg
    12001 cgacccggat tttgtcaaag taccgtggca ggtgctgacc aataaagcct atgccaaatc
    12061 tattgccgat caaatcgata tcaataaagc gaagccatcc agcgaaattc gtcctggcaa
    12121 gcttgcgcct tatgagagta atcaaactac ccattactca gtagtggata aagacggtaa
    12181 cgccgtggcg gtgacctata cgctgaacac caccttcggt acgggcattg tcgcgggcga
    12241 gagcggtatt ttgctcaata accagatgga tgatttctcc gccaaaccag gcgtaccgaa
    12301 cgtttacggg ctggtgggcg gcgatgccaa cgccgtcggg ccgaataaac gccctctgtc
    12361 gtcgatgtca ccaaccattg tggtcaagga tggtaaaacc tggctggtca ccggaagccc
    12421 aggcggtagc cggattatca ccactgtgct gcaaatggtg gtgaatagca tcgattatgg
    12481 aatgaacgtc gccgaagcga ccaatgcgcc acgtttccac catcagtggt tgccggacga
    12541 gctgcgtgtc gaaaaagggt ttagcccgga tacgatcaag ctgctggaag caaaaggtca
    12601 gaaagtggcg ctgaaagagg cgatgggcag tacacaaagc attatggttg ggccggacgg
    12661 tgagctgtac ggtgcatccg acccgcgctc ggtggatgat ttaacggcgg ggtattaagg
    12721 ttagcggccc tcttcgtggg aagagggccg tattgtcagg gcaagccgaa ggtagccttt
    12781 tttatttcat aatcctgtag atattcttcc agcggctttg ccgttccaag ggaatgaatt
    12841 tcaccactgc ctttatcaac aataaaaggt gcgttaccag ctaagcgcgc ggcctcatct
    12901 ccagtttcga aaaattctcg tgcttcgaaa cagaaatacc agccctggct aaagcgtcca
    12961 tgtagagtaa tgacgaccgg gagatttgca tcatcaaggt aattgttcgc tttcgcgaat
    13021 gcgtcgtgat aagtaatcat aattataata aatattattg tttagcgtta tattattatt
    13081 cctgcgttat acattgagca aagacgcgtt catcttcgtc aaagatatta ttaaatgctg
    13141 gttttgaaaa gagttcatcg gataaggtat agtcacaaaa taagtatctg ctaatatttt
    13201 gtgatttatt ttccagaacg tcgaaccgga tttgctcatt taagatatca gaatcttatg
    13261 attcgggtaa aattctaacc aaatcaacat gtcactaaga tcatcatcgc cattagctat
    13321 ttcagtttaa atagcgacta catgtcttcg gtccatctca cttaaggagt gtagttccgt
    13381 tgtaagtttt tctatagctt gtattgataa atttcgatca agaaatttac tgctggtaaa
    13441 ctctaaaaag atggcatggt ttacaatgat tttggttacc tttttattat tatgaacaac
    13501 tgtccatgat ttcgtttaag aatg
//
LOCUS       DAZFFE010000118        13293 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFFE010000118 DAZFFE010000000
VERSION     DAZFFE010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.118"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            237..1334
                     /gene="mltA"
                     /locus_tag="ACHJ69_003959"
     CDS             237..1334
                     /gene="mltA"
                     /locus_tag="ACHJ69_003959"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO5016842.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGVDNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1411..2217
                     /gene="tcdA"
                     /locus_tag="ACHJ69_003960"
     CDS             1411..2217
                     /gene="tcdA"
                     /locus_tag="ACHJ69_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO5016843.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVVQYMNAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2268..2711)
                     /gene="csdE"
                     /locus_tag="ACHJ69_003961"
     CDS             complement(2268..2711)
                     /gene="csdE"
                     /locus_tag="ACHJ69_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO5016844.1"
                     /translation="MTNLQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPEELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2711..3916)
                     /gene="csdA"
                     /locus_tag="ACHJ69_003962"
     CDS             complement(2711..3916)
                     /gene="csdA"
                     /locus_tag="ACHJ69_003962"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO5016845.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNASDDKNIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKPELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLTDYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4108..4335
                     /gene="ygdI"
                     /locus_tag="ACHJ69_003963"
     CDS             4108..4335
                     /gene="ygdI"
                     /locus_tag="ACHJ69_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO5016846.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4686..5603
                     /gene="gcvA"
                     /locus_tag="ACHJ69_003964"
     CDS             4686..5603
                     /gene="gcvA"
                     /locus_tag="ACHJ69_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO5016847.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5622..6017
                     /gene="ygdD"
                     /locus_tag="ACHJ69_003965"
     CDS             5622..6017
                     /gene="ygdD"
                     /locus_tag="ACHJ69_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO5016848.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6010..7110
                     /gene="rlmM"
                     /locus_tag="ACHJ69_003966"
     CDS             6010..7110
                     /gene="rlmM"
                     /locus_tag="ACHJ69_003966"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO5016849.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSSNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7154..7885)
                     /gene="fucR"
                     /locus_tag="ACHJ69_003967"
     CDS             complement(7154..7885)
                     /gene="fucR"
                     /locus_tag="ACHJ69_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO5016850.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKREHIKLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINTDYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVTTSLVTA"
     gene            complement(7943..8365)
                     /gene="fucU"
                     /locus_tag="ACHJ69_003968"
     CDS             complement(7943..8365)
                     /gene="fucU"
                     /locus_tag="ACHJ69_003968"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO5016851.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8367..9785)
                     /gene="fucK"
                     /locus_tag="ACHJ69_003969"
     CDS             complement(8367..9785)
                     /gene="fucK"
                     /locus_tag="ACHJ69_003969"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO5016852.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISEQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQILIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTTQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9894..11669)
                     /gene="fucI"
                     /locus_tag="ACHJ69_003970"
     CDS             complement(9894..11669)
                     /gene="fucI"
                     /locus_tag="ACHJ69_003970"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO5016853.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVRKPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGKPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11702..13018)
                     /gene="fucP"
                     /locus_tag="ACHJ69_003971"
     CDS             complement(11702..13018)
                     /gene="fucP"
                     /locus_tag="ACHJ69_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO5016854.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     FGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSTYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLAHIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         3218 a         3292 c         3486 g         3297 t
ORIGIN      
        1 acgagctacc aggctgctcc accccgcgtc cgtggatgcg cactatactc tgctcgcgtt
       61 ttcatgcaac ctttttttta ccccgatcac ggattcggca tgattttgaa caaaaacaaa
      121 tcactaaagc ctattttttg tgcaaaattt gcgtcaggga gaggctgctc atttcattaa
      181 acgcggtggt ttgttatctt cgttgcgcct tattttttaa cctgaagaag agaacaatga
      241 aaggacgttg ggtaaagtac cttcttatgg gcacggttgt ggcaatgctt gccgcctgct
      301 cttccaaacc aaccgatcgc ggacagcaat ataaagacgg gaaatttacc cagcctttct
      361 ctctggtgaa ccagccagat gccgttggcg cgccgattaa tgccggtgat tttgccgagc
      421 aaattaacca tatccgtaat tcgtcaccgc gtctgtatgg caaccagagt aatgtttata
      481 acgcggtgca agagtggctg cgcgcaggcg gtgatacccg caatatgcgc cagttcggca
      541 ttgatgcctg gcagatggaa ggtgtcgaca actatggtaa cgtgcagttt acaggctatt
      601 acacgccggt aattcaggcg cgccataccc gtcagggcga gttccagtat ccgatttacc
      661 gtatgccgcc aaagcgtggt cgtctgccgt ctcgtgcgga gatctacgca ggagctctga
      721 gtgataaata tattctcgct tacagtaact ccctgatgga taacttcatt atggatgtgc
      781 agggtagcgg gtatatcgac tttggtgatg gcagtccgct taactttttc agctatgcag
      841 ggaaaaacgg tcatgcctat cgcagcattg gtaaggtgtt gatcgaccgt ggcgaagtga
      901 aaaaagaaga tatgtcgatg caggcgattc gtcactgggg cgaaacacat agtgaagctg
      961 aggttcgcga gctgctggaa cagaacccgt ctttcgtctt ctttaaaccg caatcttttg
     1021 caccggtgaa aggggcaagt gcggtgccgc tggttggccg tgcttctgtt gcctccgatc
     1081 gttcaattat tccgccaggt actaccttgc tggcagaagt gccgctgctg gataataacg
     1141 gcaaatttaa cggtcagtac gaattgcgtc tgatggtggc gctggatgtc ggtggcgcaa
     1201 tcaaaggcca acacttcgat atctatcaag ggatcgggcc ggaagccgga caccgcgcag
     1261 gttggtacaa ccactatgga cgtgtctggg tgctgaaaac cgctccgggc gcaggtaacg
     1321 tctttagcgg ctgatatgat attctgtatg ccgtttttac ggatatgaca gggtgagggg
     1381 taacctcacc ctttttaatt ctgaggtgct atgtctgtgg taattagtga tgcatggcgt
     1441 cagcgttttg gtggcacagc gcgtctgtat ggtgaaaaag cattgcaact gtttgctgac
     1501 gcgcatattt gtgtggttgg tatcggtggt gtcggttcct gggcggcgga agcgctagcg
     1561 cgcacgggga ttggcgcaat cacgcttatc gatatggatg atgtgtgcgt caccaatacc
     1621 aatcggcaaa ttcacgctct gcgcgataac gtcgggctgg caaaagcgga agtaatggcg
     1681 gagcgtattc gccagattaa cccggagtgc cgtgtaacgg tggtggatga cttcgtgacg
     1741 ccggataacg tagtgcaata tatgaatgcc ggttattcgt acgtgattga tgctattgat
     1801 agtgtacggc ccaaagcggc gctgattgct tattgtcggc gcaataaaat tccgctggtc
     1861 acgaccggtg gcgctggtgg gcagattgac ccgacgcaga ttcaggttac cgatctggcg
     1921 aaaacgattc aggacccgtt ggcggcgaag ttgcgcgagc gcctgaaaag cgattttggc
     1981 gtagtgaaaa acagtaaagg taagctcggc gtggattgcg tgttttctac cgaagcgctg
     2041 gtgtacccgc agtcagacgg caccgtctgt gcgatgaaag ccacggcgga agggccaaag
     2101 cggatggatt gtgcctcagg atttggcgcg gcgacgatgg tgaccgccac ttttggcttt
     2161 gttgcggttt ctcatgcgct gaagaagatg atggcgaaag cggcgcgtca ggggtaaaca
     2221 atcaggtcgg atgcgacgcg aacgtcttat ccgacctggt tctgtgctta aacctgcttc
     2281 gccgcagcga taatcgcctc gcttaacgca ttcaacccct ggctgcgtga ggcgctaagt
     2341 tgcgcacgta atcccagctc atcaaacaat gccagcggcg actgtgcctg caattcggcg
     2401 gcggttttcc cctcaacggc agtcaacaac accgccaaca ggccgcgcac aatgcgccct
     2461 tcgctgtcgc caaagaaatg catcttgccg ttttcagcca cggtatatcc cagccagacg
     2521 cggttttcgc atccggcaat ctctttagcc tgcgctttta actcctctgg caaagccgga
     2581 agctgtttcc ccagcatgat caactggcga tatttatctt cccattgcgt caacggtgcg
     2641 aaggtattgc gtaacgtttc tgcggttacg gttgtgccga acggatgtcc ggcgaattgc
     2701 aggtttgtca ttaatccacc aataattcca gcgcgcggtc aacggcattc accagcgcat
     2761 ccacatcact ctttgtatta tatggcgcaa atgaggcgcg cagtgtgccg gttacgccta
     2821 attctgccag tagcggctga gcgcaatgct gcccggcgcg cagggcaata ccgtactccg
     2881 ccagcagtgt caccatatcg ctgtgatgaa ctccagcaaa atcaaaggcc agcaggctgg
     2941 aatcctggca gcggaatgaa cgaaagcctg gacgtttcgc cagcgcttct tctgcaagcg
     3001 ttgctaagct acggctccag ctttcggcct ggttgatatc gtaatctgtc agccattcca
     3061 gcgccgcgct taatcctatg acaccagcga catttggcgt tcctgcttcc agtttccacg
     3121 gcgcagattg agtcgtgaag ccgtcaaaac tcacttcgtg aaccattttg ccgccgccca
     3181 gccagggcga catcgcttcc agcagttctg gtttgccata cagcacgccg atgcctgtcg
     3241 gaccatacag tttgtgacct gaaaaagcat agaaatcaat atccagttgc tgaacatccg
     3301 cggggaaatg caccgccccc tgagcaccat caaccataac caccatcccg gctgaatgag
     3361 caaaagtaat cgctctcgcc agatccgggc aaccgccagt gacgttcgac atctggccca
     3421 acgccagaat ccgactacgg ggagtaatca gttctggcaa caaatcgaca tccggcagtc
     3481 gctgcgcatt aagcggcaat ttcaccactt tggcccccgt ttgttgggcg accatcagcc
     3541 aggggacgag gttggcgtgg tgttctgcca cactgacaat aatttcatcg cccggttgca
     3601 gacgcggacg ggcatagcat tgcgccacca tgttgatgga ttcagtggtg ccgcgcgtcc
     3661 agacgatatt tttgtcatcc gatgcattca gtaattgtgc cactttttcc cgcgcagctt
     3721 cgtaacgcgc ggtcaggcgt tgggcttcgg caaactggct gcgatggacg tttccggcgc
     3781 tcaggctata aaactgtcgg gtagcttcaa ccacggcttc aggtttaagc gcggtcgcgg
     3841 cgctgtcgag atagacgccc gcatcctgta gcgcgggaaa ctgggcgcga aactgcgcgg
     3901 gattaaaaac gttcatggta ctcctcggct tgatgcaccg atcgtgacgc aaaattcact
     3961 ttgatacaag gagagtgcta accatcagaa ttatctggat atcctggcga agccgcgtca
     4021 gtaacatatg ctgaatagtg ttaggcgtta attttagcct gttaatcaat actattaaga
     4081 tttattaatc gctttaagga gaagatgatg aaaaagactg ccgcaattat ttctgcctgt
     4141 atgctgactt ttgccctgag cgcctgttcc ggttcgaact atgtgatgca caccaatgac
     4201 ggacgtacca tcgtctctga cggcaaacca cagactgata acgataccgg tatgatttcg
     4261 tataaagacg ctaatggcaa caaacagcag atcaaccgta ctgacgtgaa agagatggtc
     4321 gaactggatc agtaattcgc gatcgcaagg taaaaaaaag caccgcaatt aggcggtgct
     4381 acattaatca ctatggacag acagggtaaa tgtacaggaa gtgaaaaaag gtagctttgc
     4441 taccatggtc tgaatcgcag accaattgca aacacaacaa cacaacatca caaccgtaag
     4501 ccaaaagttc accagaacac gcattccgat aaaacttttc gttccggctc aggaagtgcc
     4561 gccactatag gtatttgctg gtagaagctc aacggacaat ttataatggc tcagattaaa
     4621 aaaactaata ggttacacag tgtgatctaa ttgttaaatt catttaacat caaagtttaa
     4681 aagccatgtc taaacgatta ccaccgctaa atgccttacg agtttttgat gccgcagcac
     4741 gccatttaag tttcactcgc gcagcagaag agctttttgt gacccaggcc gcagtaagtc
     4801 atcaaatcaa gtctcttgag gattttctgg ggctaaaact gttccgccgc cgtaatcgtt
     4861 cactcctgct gaccgaggaa gggcaaagct atttcctcga tatcaaagag atattttcgc
     4921 aattaaccga agcgacgcgt aaactccagg cccgtagcgc taagggggcg ttgacggtca
     4981 gtttactccc cagtttcgcc attcattggt tggttccgcg actttccagc tttaattcag
     5041 cttatccggg aattgacgtt cgaatccagg cggttgatcg tcaggaagat aagctggcgg
     5101 atgatgttga tgtggcgata ttttatggtc ggggcaactg gccggggcta cgggtggaaa
     5161 aactgtacgc tgaatattta ttgccggtgt gctcgccgct actgctgact ggcgaaaaac
     5221 ccttgaagac cccggaagat ctggctaaac atacgttatt acatgatgcg tcacgccgtg
     5281 actggcagac atatacccga cagttggggt taaatcatat caacgttcag caagggccaa
     5341 tttttagcca tagcgccatg gtgctgcaag cggctatcca cgggcaggga gtggcgctgg
     5401 caaataacgt gatggcgcaa tctgaaatcg aggccggacg tcttgtttgc ccgtttaatg
     5461 atgttctggt cagtaaaaac gctttttatc tggtttgtca tgacagccag gcagaactgg
     5521 gtaaaatagc cgcctttcgc caatggatcc tggcgaaagc cgctgctgaa caagaaaaat
     5581 tccgctttcg ttatgaacaa taatttacgt agggtacgac catgaccagc cgttttatgc
     5641 tgattttcgc cgccattagc ggcttcattt ttgtagctct gggcgctttt ggcgcgcatg
     5701 tgttaagtaa aaccatgggg gccgttgaga tgggctggat ccagaccggc ctcgaatacc
     5761 aggcgtttca tacgctggcg atcttaggtc tggcggtggc aatgcagcgt cgcatcagta
     5821 tctggtttta ctggagtagc gttttcctcg cgttaggcac ggtgctgttc agcggcagcc
     5881 tttattgcct ggcgctgtcc catctgcgtt tgtgggcgtt tgtcacaccg gttggcggcg
     5941 tgagcttcct tgcgggctgg gcgttaatgt tagttggtgc tatccgttta aagcgcaagg
     6001 gcgtaagtca tgaataaggt tgtattgctg tgccgtccgg gctttgaaaa agagtgcgcc
     6061 gcagaaatta ccgataaagc cggccagcgg gaaattttcg gttttgcccg cgtgaaagag
     6121 aatgcgggtt atgtcattta tgaatgttat caacctgatg atggcgataa gttaatccgt
     6181 gagctgccgt tcagttcatt aatttttgcc cgccagtggt ttgtagtggg ggaactcctg
     6241 cagcatttgc cgccagaaga tcgtattacc cccattgtcg gcatgttaca gggcgtagta
     6301 gagaagggcg gtgaactgcg tgttgaagtt gccgatacca acgaaagcaa agagttgctg
     6361 aaattctgcc gtaaattcac cgtgccgcta cgcgctgcct tgcgtgatgc gggggtgctg
     6421 gcgaactatg aaacgccgaa gcgtccggtt gtgcatgtat tctttattgc accaggctgt
     6481 tgctatactg gttactcata cagcagcaat aattcgccgt tctatatggg cattccgcgc
     6541 ctgaaatttc cggcagatgc gccgagtcgt tccacgctca aactggaaga ggcatttcat
     6601 gtgtttattc ccgcggatga gtgggatgaa cgcctggcga acgggatgtg ggcggtggat
     6661 ttaggtgctt gcccaggcgg ctggacctac caactggtga agcgcaacat gtgggtttat
     6721 tccgtcgaca acggcccgat ggcgcaaagt ctgatggata ccgggcaggt aacgtggctg
     6781 cgggaagacg gtttcaaatt ccgtccgaca cgcagcaata tctcctggat ggtatgcgat
     6841 atggttgaaa aaccggcgaa agttgcggca ttaatggcgc agtggctggt taatggctgg
     6901 tgccgtgaaa ccattttcaa cctcaaactg ccgatgaaaa aacgctacga agaagtgtca
     6961 cacaatctgg cgtatattca ggcacagctt gatgaacatg gcataaatgc tcagattcag
     7021 gcgcggcagt tgtatcacga tcgcgaagaa gtgacggtgc acgtccgccg tatctgggct
     7081 gcggtgggtg gtcgtcgcga cgagcgataa caggtaaggc cggggcgtcg tctccggcct
     7141 gctacccttt ccgtcaggct gttaccaaag aagttgtaac ctggcgctca tcagaaataa
     7201 tgtgcgtgac ctcatccaga tgcccgatgc gggcttcccc tgaacgatta aatttacttt
     7261 tatcaatcaa taacaacgat tgcgcggcac gttttaataa cattgatttg taatcagtgt
     7321 tgatggcatt ggagtcccac agtgcgccgc tgctatcgat cccttcacaa gaaaaaataa
     7381 acagatcgat ttccagcgat ttaagttggg aaatcagcga gggattgacg taacagccat
     7441 atttgcgctc aagtgtgccg ccggaactga tcagtttaat gtgttcgcgt ttaccgagtt
     7501 catggcaaat cggatggcta ttggtgaaga cctggatgtt gatgtcaggc aactggcgtg
     7561 ccagatacca gcaggttgaa ctggcgtcta aggctatcac catcccttct tcaatccacg
     7621 cgagcgcctc gcgcgcgata tctgctttat gcgcataatg gcttttcagc cggatgtgaa
     7681 agggatcgcc actgtcttga ttttgacggt ggatatattt agcgcgccca tgattgcgca
     7741 gaattttacc ctgcgtctgt aattcattga gatcgcgacg aatggtttct ttactgacct
     7801 ttagctgttc agagagagct tccgtggtca ggctggtatg gttcagcagc aggtcgacta
     7861 tcgcttgctg gcgtgccgct ttcatatttt tcaccccatc attcgataac cgcccgcgtc
     7921 atgcgggcgt accggtatga gattacggtg ttaccccttt ttttaaaaga atattcccgt
     7981 acttcgctcg ttcgcctgtg ataacgatcg caaaggcttt ttgcgcccgt tcataaaacg
     8041 caaaacgatt gatgcggatg atgtcaggac agggggcttg tagtgaaagc gcattacgat
     8101 aacgtcgttc tacttcagga tcgagagtgt caccttctac cgccgccatc atcaccagcg
     8161 gtggtgcata actgtccagc tcaaataacg ggataatcgc ctggagcaag tcgctcacca
     8221 acaggccatc agcgcggatc acctgcggtc ccatcgaatg ggcgggaaag tgagcatcgg
     8281 aaaaaataat ttcatctcca tgccccatct ctgccagcac tttcaatagt tcgggagaaa
     8341 ttaacggcga aattgttttc agcatttcac acctcctcta taaattcagg ttcagtttgc
     8401 gggtagaaat aacggtactg ataatgaatc tgtgcacggg cttcttccgg gctgttaaat
     8461 tcccctacgc cataccaacc aaacagcgca gctcctgcga cggtcgtttc ggcatcgtcg
     8521 agaactttta ccggaatatc aagcatattg gctttaatct gattccacaa tgtgttgcga
     8581 cttcctccgc cgactaacaa taattcagag gctttaaagt gcccgatttt ttccagcatc
     8641 tgtagattgc gctgtaattg cgtggttaac ccttccagcg ccgcgcgata gaaatgcccc
     8701 cgcgtggtat taagcgttac gccttgccag ccagcgttct ggcacgacaa taaatcacac
     8761 tgcattttca ctccatctgc gccaggcgcg atcaggcgag cttcttcaat caatatttgc
     8821 cagggagttt cagccgtcca gaacagtttt ctcacccatt ccagcacgcc ggatgccagc
     8881 cattgcatac ctgggttata caatcctgcc tgactatcca gttcgcaggt cgaaccggcg
     8941 tactgactta acagcgaagt atcaacctgg gcgctgcgga ccattaaaat ttcccatgta
     9001 ccggaagaga gcacgggttc attttgttcg gcaccagcgc caaaaagggc gaactgggta
     9061 tcgtgacctg cggaaatcac cggtatgcca acgggtaagc cgagcattgc tgcggcgctg
     9121 ttctgtagcg taccaatctg ttcacccgct tccaccagac gagggaagag tcggcgtgga
     9181 ataccggtgg cttgtaaaat ttgcggactg aaatcgcgtt gctggatatc cagcatctgg
     9241 ctggttccgg ccatcgtgat atcagtagtg aattcgccgg ttaaacggtg gttaatcagc
     9301 gacgaaataa agagccaggc gtgcgcgcgt tccagcagtt gtggatgatt ttctttcaac
     9361 cacaccaact tatataacgt attgaaacta aaggctccga ctccagaaat agcctgcaac
     9421 tgctgttcgg agatcaaccg ttcaatattg tccattaccg cggctgttcg cggacatttc
     9481 cagctaatga tcggatagag cagattgcct tgcttatcta ccagagcgcc atccacacca
     9541 aaggtggtga cggcgatacc gcggatgtgg caatcagtca gttcactatt gatttgccga
     9601 cagcaatcag caaagcgttg caaaatggcg tctaaagacc actggtgcca ggtgttgttt
     9661 tccatcgcga tatcgctggc attaggcgtt gaggcgcggg caacaatttt tccctgccga
     9721 ttgaccgcga tggccctgac attggtcgcg ccacagtcga gtaccaggat aacttcttgt
     9781 ttcataatcg ctcctgcaat atagccggat aacattgctt atccggctaa ccactcttgc
     9841 ttgttcaggc ccggtagccg gagcgaccgg gcatcacatc agggagtaat gtattaacgc
     9901 ttgtacaacg ggccgtagtt ctggcaagcg cggtaatcct ggccttcaat atccatgccg
     9961 tgcgcggccc aggcagaagg acgatacact ttggtctctt caacgttgtg catacatacc
    10021 gggatacgca gcatggaggc aagagtgata aagtctgcgc caacgtggcc gatggtcaga
    10081 accccgtggt tagcgcccca gttcgccatt accgagtaca catccgtaaa cggcccttta
    10141 ccggtgaggc gcggtgcaaa ccaggtggtt ggccaggttg agttggtgcg tttgttgagg
    10201 atgtcatgca catccttcgg caattccacg ctccagcctt ccgcgatttg cagtaccggt
    10261 cccaagcctt tgatgatgtt gactcgagtc atagtgaacg ggacgccgcc ttcggtaagg
    10321 aagcgagaag agtaaccgcc gccacggaag tattcgtgga tcgccgggca ccattcggta
    10381 gcggcgaggc aagcgtcagc ctcttgctga gagatttccc agtgcggctt catcgtcggt
    10441 ttaccttcgc tgtcgcgttg tttacaggag ccgtccagcg cagcagaacc ggagttgatc
    10501 aaatggatga tgccgtgttc tgccagcccg tccagtttat gccccgttac acgctcaatt
    10561 gcttctggtg accagtaggt acggacatcg gcaaatacct gagcggtgcc ggtgagctgg
    10621 tggcccatta acattgccac gccgttaaga ctgtcgtttt cggtcgccac gacaaacggt
    10681 ttgcgcacgc cattccagtc aaatgaactg ttgaggatcg cttcggcggt gtcgccattg
    10741 ggatattgat cggtccagtg acgttgcccc tggaagcccg cagcgatggc gttgtagcca
    10801 agtgattctt ccacgcggcc aatatcggcc agtttgctgt tgccttgcat catgtcgcgg
    10861 atacacatcg ccatcagtaa actttcgcgc agaactgcgc ggctttgctc ggcatttcgt
    10921 tgatactgtt tgttattttc atcttcgcca tagcggaagt ttttatcagc ccaggccagc
    10981 gccatttcca gttcggcttc gtcgtaaatc ttctgatcga tacggcgacg cagttcggtc
    11041 atatccaccg cctggacttt cattcccagc caggattcaa agaagttgtg atcaacaata
    11101 gaaccggcga tacccatcga aacgccgcct agcgacagat agcttttacc tttcatgctg
    11161 gcgacggcca aaccggcgcg ggcaaaacgc agcagttttt cttcaacatc ggcaggaatc
    11221 gatgtgtcat cggcatcctg aacgtcatga ccgtaaatgg agaatgccgg gatgcctttc
    11281 tggctgtgag ctgccagagc cgctgccagg taaacagcgc cgggacgttc agtgccgtta
    11341 aagccccaaa tggctttcgg gcgggttgga tccatgtcga tggtttcact gccatagcac
    11401 cagcaagggg ttaccgtaat ggtgaggcct acattctgac tgctgaattt ttcttcgcaa
    11461 gcagcggctt cagccatacc cgcgatacag gtatcggaaa tgacacactc gacggcagct
    11521 ccacaggcat ggcgcagttt ctcggtcagc agtgcggccg tggctttcgc catattcatt
    11581 gtttgttctt caagcgactc acgaacaccc atgcgacgac cgtcaataac cgggcggata
    11641 ccaattttcg gtaagctgat ttttttcatt acagattcct cactttattc ggaaaatatg
    11701 ttcagttagt tgccgtttga gaacggaaac gggcaaagat aaagatgact gcgaagcaga
    11761 gtgccgggat cagttcggct gtggggatgt tgcccgcagc gtcactgaca aaccccatga
    11821 ccggagtgac aataccgccg ccaataatgg tcataacgat gaaggacgaa ccgtatttgg
    11881 tgtcctggcc gagattctta atgcccagcg agaagattgt tgggtactga atcgacataa
    11941 aggcactgca taaagtcagg gctattaaac ccacatgacc gccagcgaag gctgagatca
    12001 ggcacagtgc catagcaatt aatgcgtagg ctgccaggac tttgtgtggt gcgaagcgac
    12061 tgatgagcca ggtaccggtg aaacgaccaa taaagaagca caccatggtt ccggttaaat
    12121 agttagcggc aaagcctgct gtcatacctg gaatttcttc tacagcgtag cgaatcaaat
    12181 agctccagca ggccgtttgt gcgccgacgt agcagaattg cgctaatact gcccagcgcc
    12241 agtggcgaat atgcgccagg cgagaaagcg atgcggagaa cgatccttgt ttggcgtcac
    12301 tgtgattatc actctgcaat gccgggaatt tcgtcagcat gatcagcagg gcgaccagta
    12361 acacgatagc cacgatgatc atataaggtg tctgtaccga taatactagg ctgtgtttat
    12421 acgtactcaa ttgctctgga gacattttat cgagaacgtc ttgcgattga tgtggcacgt
    12481 tagacaaaat aagactttgc ccaaagacaa ccgcgataat tgcgccaaat gagttaaatg
    12541 tttgcgcaag atttaagcgg aagtgaccac tgctttccgg ccctaatacc gtaacaaaag
    12601 ggtttgcggc agtttccaga caacctaatc cggctgcaat aataaatagg ccaactaaaa
    12661 acaaggtgta gttcattatt tctgcggcgg gccagaataa tgcagcgccg aaagcatata
    12721 aaaacaatcc ggtaataatc cctgctttat aactgagttt tttcatcaat atcccggcag
    12781 ggattgggat aatgaaataa ccaaagtaaa aggccgattg gatcaggcca gcctggaaat
    12841 ttgtcagcgt aaaagcctgc tggaattgag gcaataaaat gtcgttaagg ttattggcta
    12901 ccgcccaaag aaaaaacagt gagcacagca gcgcgaatgg aataatgtaa cttctgcttt
    12961 gccctgcatc tttatctacc gcacggtaac tctgcgtttg tattgatgtg tttcccatag
    13021 catcctctta ggttcagaag cttaagcacg taatcggtta tgttttcaaa agtgcaaaac
    13081 cagcgagttc gggcttcata tcagtgaata tttgcctgtc tgaaatgaac tcatggattc
    13141 attttcaaat aaaaaacgct ctgtaatatg acggcggtca cacttattag ccggaaggtg
    13201 actttatgtg actaccatca ctttaataat cgaaatttca tgcccgtttc agaaaataaa
    13261 tcataaaaaa cacaaaccag atgaccgtat taa
//
LOCUS       DAZFFE010000119        12276 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZFFE010000119 DAZFFE010000000
VERSION     DAZFFE010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.119"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            49..1410
                     /gene="allB"
                     /locus_tag="ACHJ69_003972"
     CDS             49..1410
                     /gene="allB"
                     /locus_tag="ACHJ69_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO5016855.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKDGKIAAIGQDLGDAKDVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEMGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHVSSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            1466..2767
                     /locus_tag="ACHJ69_003973"
     CDS             1466..2767
                     /locus_tag="ACHJ69_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFO5016856.1"
                     /translation="MFNFAVGRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGQGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            2789..3934
                     /gene="glxK"
                     /locus_tag="ACHJ69_003974"
     CDS             2789..3934
                     /gene="glxK"
                     /locus_tag="ACHJ69_003974"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO5016857.1"
                     /translation="MKIVIAPGSFKESLSAEKCCQAIKAGFSTIFPDAHYICLPIADG
                     GEGTVEAMVAATDGNIVKLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQA
                     LAANGGNLARVASIEMNECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQLTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(4063..4848)
                     /gene="allE"
                     /locus_tag="ACHJ69_003975"
     CDS             complement(4063..4848)
                     /gene="allE"
                     /locus_tag="ACHJ69_003975"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO5016858.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFT
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYIPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(4859..6094)
                     /gene="allC"
                     /locus_tag="ACHJ69_003976"
     CDS             complement(4859..6094)
                     /gene="allC"
                     /locus_tag="ACHJ69_003976"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO5016859.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFK
                     KRMTASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDATVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(6116..7165)
                     /gene="allD"
                     /locus_tag="ACHJ69_003977"
     CDS             complement(6116..7165)
                     /gene="allD"
                     /locus_tag="ACHJ69_003977"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO5016860.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QKGVAVVGISRMGHSGAISYFVQQAARAGLIGISLCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELLRQHLSQTMRELNTITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            7482..9149
                     /locus_tag="ACHJ69_003978"
     CDS             7482..9149
                     /locus_tag="ACHJ69_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO5016861.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAVMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAETVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSSATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            9159..10418
                     /locus_tag="ACHJ69_003979"
     CDS             9159..10418
                     /locus_tag="ACHJ69_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO5016862.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNKGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGASLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            10429..11244
                     /locus_tag="ACHJ69_003980"
     CDS             10429..11244
                     /locus_tag="ACHJ69_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO5016863.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYYLLRITPP
                     AHPQQLAVAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAAAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            11241..12134
                     /locus_tag="ACHJ69_003981"
     CDS             11241..12134
                     /locus_tag="ACHJ69_003981"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO5016864.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPHVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEATYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
BASE COUNT         2757 a         3044 c         3460 g         3015 t
ORIGIN      
        1 attgaacatt gagttaaaaa ccaatctgta ttttacaagg agtttgttat gtcttttgat
       61 ttaatcatta aaaacggcac cgttatttta gaaaacgaag ctcgcgttgt agatatcgcc
      121 gttaaagacg gaaaaattgc tgctatcggc caggatctgg gcgatgcaaa agacgttatg
      181 gatgcgtctg gtctggtggt ttcgccgggc atggttgatg cgcacaccca tatttctgaa
      241 ccgggtcgca gccactggga aggttatgaa accggtactc gcgcagcagc aaaaggtggt
      301 atcaccacca tgatcgaaat gccgctcaac cagctgcctg caacggttga ccgcgcctca
      361 attgaactga agttcgatgc cgctaaagga aagctgacta tcgatgcggc acaactcggt
      421 ggcctggtgt cttacaacat tgaccgtctg catgagttgg atgaaatggg tgttgtcggt
      481 ttcaaatgct tcgttgcgac ctgtggcgat cgcggtatcg acaacgactt ccgtgacgtc
      541 aatgactggc aattcttcaa aggcgcgcag aagctgggtg aactgggcca gccagtgctg
      601 gtgcactgcg aaaacgcgct gatctgtgac gcactgggcg aagaagcgaa gcgtgaaggt
      661 cgcgtaactg cccatgacta tgtggcttcg cgtccggtat ttaccgaagt ggaagcgatt
      721 cgccgcgtac tgtacctggc gaaagtcgct ggttgccgtc tgcacgtttg ccacgtcagc
      781 agcccggaag gtgttgagga agtgactcgt gcacgtcagg aaggtcagga tgttacttgt
      841 gaatcctgcc cgcattactt tgtgctggat accgatcagt tcgaagaaat cggtactctg
      901 gcgaagtgtt caccgccgat ccgcgatctg gaaaaccaga aaggcatgtg ggaaaaactg
      961 tttaacggtg aaatagactg cctggtttcc gaccactctc catgcccgcc ggaaatgaaa
     1021 gccggtaaca tcatgaaagc gtggggcggt atcgctggtc tgcaaagctg catggacgtg
     1081 atgttcgatg aagcggtaca gaaacgcgga atgtctctgc caatgttcgg caaattaatg
     1141 gcgactaacg cagcagatat tttcggtctg cagcaaaaag gccgtatcgc cccaggaaaa
     1201 gatgccgact tcgtcttcat tcagccgaat agcagctatg ttcttaccaa tgacgatctg
     1261 gaatatcgcc acaaagtcag cccgtatgtt ggccgtacta ttggcgcgcg tatcacgaaa
     1321 accatcttac gtggtgatgt gatttacgat atcgaacagg gcttccctgt tgcgccgaaa
     1381 ggtcaattta tccttaaaca tcagcagtaa tctggccctg caatgcccgt ccttgtggcg
     1441 ggcattctcc ggttaaggtg tgtttatgtt caattttgca gtgggccgcg aaagcctgtt
     1501 atcaggattt cagtggtttt tctttatttt ttgcaacacg gttgtggttc ctcctacgct
     1561 actttctgct tttcagttgc cgcaaagtag cctgcttacg ctcacgcaat atgcttttct
     1621 tgctaccgca ctggcctgct tcgctcaggc gttctgcggt catcgtcgcg ctattatgga
     1681 ggggcagggc ggcctgtggt ggggaaccat cctgactatc acccttggcg aagcatcgcg
     1741 cgggacaccg atcaacgata tcgctaccag tctggcggtg gggattgcgc tctccggcgt
     1801 gctgacgatg ttgattggtt ttagcggatt aggccatcgc ctggcacggt tatttacgcc
     1861 gtcggtgatg gtcttgttta tgttgatgct gggtgcgcaa ctgaccacta tctttttcaa
     1921 aggtatgctc gggctgccgt ttggcatagc cgacccgaat tttaaaattc aattaccgcc
     1981 gttcgcgctc tcggtggcgg tgatgtgcct ggtactggcg atgattattt tcctgccaca
     2041 acgttttgcc cgttacggcc tgctggtcgg tacgataacc ggctggttgt tgtggtattt
     2101 ttgctttcct tcttcgcatt cgctctccgg tgagttgcac tggcagtggt tcccgctcgg
     2161 cagtggcggt gctttgtcgc cggggattat tctgacggcg gtgattacag gtctggtaaa
     2221 tatcagcaat acctacggtg cgattcgggg cacggatgtt ttttatccgc agcagggcgc
     2281 ggggaatacg cgttatcgtc gcagctttgt ggcgaccgga tttatgacac tgataaccgt
     2341 gccgctggca gtaattccat tttcaccgtt tgtttcatcc attggtttat taacccagac
     2401 aggcgattac acgcggcgtt cgtttattta tggcagcgtt atttgcctgc tggtggcact
     2461 ggttccggca ctcacgcgac tgttttgcag tatcccttta cccgtgagta gtgcagtcat
     2521 gctggtttct tatctgcctt tactcttttc cgcgctggtg tttagccagc aaataacgtt
     2581 taccgctcgc aatatttatc gactcgcatt gccgttattt gtcggcatat ttttaatggc
     2641 attaccgcct gtgtatctgc aagaccttcc attaacgctt cgtcctctgc tcagtaacgg
     2701 cttactggtc ggaattttac tggctgttct tatggataat cttattccgt gggaacgcat
     2761 cgaataattt gttgaaaaag gattgataat gaagattgtc attgcgccag gctcttttaa
     2821 agagagctta agtgcagaaa aatgttgtca ggcaattaaa gccgggtttt cgaccatatt
     2881 tcccgatgcg cactatatct gtttgccgat agcggatggc ggcgaaggga cggtggaggc
     2941 gatggtcgcc gcgacggacg gtaacatcgt gaagcttgaa gtctgcgggc cgatgggcga
     3001 aaaagtgaat gctttttatg gccttaccgg cgacgggaaa acggcggtga ttgagatggc
     3061 agcagcaagt ggcctgatgc tggtcgcgcc tgaaaagcgt aatccgttgc tggcctccag
     3121 ttttggtacg ggggagttaa ttcgccatgc gctggataat ggcattcgcc atattattct
     3181 cggcattggc ggcagtgcga cggtcgacgg cggtatgggc atggcgcagg cgctcggtgt
     3241 gcgtttcctt gatgccgacg gtcaggcgct ggcggctaac ggcggcaatt tagcgcgcgt
     3301 ggcaagcatt gagatgaatg aatgcgatcc gcgtctggcg aactgtcata ttgaagtagc
     3361 atgtgacgtt gataacccgc tggtaggggc acgcggcgcg gcggcagtgt ttggcccgca
     3421 aaaaggggca acgccggaga tggtcgaaga gctggaacag gggctgcaaa attacgcccg
     3481 tgttttacaa cagctaactg aaatcaacgt ctgccagatg gctggtggcg gcgcggcggg
     3541 cggtatgggc attgcggcgg cggtattcct caatgcggat attaaaccgg gtattgagat
     3601 tgtgttgaat gcggtcaatc ttgcgcaggc agtgcagggt gcagcactgg tgatcaccgg
     3661 ggaagggcgc atcgactcgc aaacggcagg cggtaaagcg ccgctgggtg tggcatcggt
     3721 ggcgaagcag tttaatgtcc cggtgattgg gattgctggc gtattgggtg atggcgtgga
     3781 agtggtgcac cagtacggca ttgatgcggt attcagcatt ttgccacgtc tggcaccttt
     3841 agccgaagtg ctcgccagcg gtgaaaccaa tctcttcaac agtgcgcgaa atattgcctg
     3901 cgccattaaa ataggtcagg gaattaaaaa ctgactatta cctttaaagc ggatgcgatt
     3961 tatatcgcat ctttttttac ctgaattgac tacaaaaaaa gccacggtat aaaccgtggc
     4021 aaaatccaac atagctaaaa ataatcaggc gagtggtatg acttaaatct ctacgtcgcg
     4081 gttgcaatct ttcgagtaaa tatagctgaa cgcttcacca cgccctacgc cataaccagc
     4141 ctgtaaagaa taagcgccca taaagatgta atcgcctttt ttcaccggga tccagttatt
     4201 gtcgaggtta taaaccccct gaccggaaag aatataggca ccgtgttcct gaacgtgggt
     4261 ttcgatataa ccgtggctgg cacctggtgc aaaagagagg atatgcatgt tcatatcaaa
     4321 acctaactct ttgggcagaa aatccagcag aataacatcg tccatgcctt cataatgaat
     4381 gcgttccagt tcgctggcat tgccagaaac cagccacggc gcatgacctt ctaccggaat
     4441 atagcgacgc ttatataaaa agatttggct gtcttcggcc tgggcgttaa caaacgtcat
     4501 taaggagcct ggcgggcaat aaagatagcc accttcactt aaggtaaatg ttttcccttc
     4561 ggctttggca gtgatatttc cagagatcac atacaggaac gtttcaatgc cttcgccacc
     4621 gaagccctgt tggttgccgc cgttttgatg cagtgtgacc agataatcaa caaaagaggc
     4681 acccagcttt ggcgtggaga ggattgtcgc gtcacaattt tcaaagcccg gaataatatt
     4741 ttttaccaga ccgtctggtg ttaacagtgc gaaattaccg tgtttcacaa tcgcacggtt
     4801 agccagtaaa tcatcgcggt aaccggtgac gttatttaaa tatcccattt ataactcctt
     4861 atttctgcca ggcaagttga taaagcatga gtgccaacgt tttgacccct tcggcaaggt
     4921 cggtaatatt ggtgcgttcc gccgggttat ggctgatccc gttgatgctg ggaatgaaaa
     4981 tcatgcaggt cggcacgcga ggcgcgaaaa tttgcgcgtc gtgcccggca ccactgtgca
     5041 tcacccggta attcagtttt tcgctttcac acaattctgt cagggtggcg accaactcct
     5101 tattcatcgg cacgggttct tcgtccatcc ataaatcgat atcaatacca atatccattt
     5161 catcgcaaat cgcccgcatg tcgttttcta actgttgggt gaaatcgcgc agcacggtag
     5221 cgtcggtatg acgacaatca atggtgaacg tggttttacc cggcactaca ttcaccgtat
     5281 tcgggcgcgg ctctactttg ccaaaggtca gaaccagcgg gtcgcccatc tttttcgctt
     5341 tttcgacaga ctgatggcaa atgcgactga aagcgtaaac cgtatcacga cgataaccca
     5401 tcggcgtggt gcctgcatgg tttgattcgc cattcagcgt cacggtataa cgacgctgcc
     5461 cgacaattgc attcaccacg ccaattgatt gcccattact ttccagcaca cagccctgtt
     5521 cgatatgcag ttcgacaaag gctttaatat cctgacgcgg agttagtggg gcgttcggca
     5581 gagtaaatcc gcaagccttc atcgcatcga caaaactatt tcctttggca tcacagatat
     5641 tccgcacgtc gtcaggattc gccagcccaa aaatattttt actgccccag aaaacatacg
     5701 ggaagcggct gccttcttct tctgccatcg ccaccacttc gaccgtacgt aacggcgcgc
     5761 cgtattgcgt tttcagccag tcaatagcca gccacgccgc cagcgcaccg aattgcccgt
     5821 cgaggttacc gccgttaacc acggtatcga tatgcgaacc gctcagaacc acttcctgtg
     5881 gatattcggt gccactcagg cgaccgtata aattccccac ttcatcgaaa cgtgtttcca
     5941 gcccgcttgc tgtcattctt tttttaaact gctgctgggt ttccagccat tccggcgaat
     6001 aaagtaaacg ggtcatccca cccgctgggt cagcgccaaa agaggaaagc cagggcagcg
     6061 tttcttctat agcttgacgg aaatgtgtaa tcataagaaa gtcctgtctc aataattatt
     6121 gcgcaaaggg atttttcgtt tcgtatgacg tgttataaag cgcgtcggaa attaaatact
     6181 ggtaaatatc atcaacaatt tcgatgcctt cgacggcggc ttttcgttgt ttaatatcct
     6241 gatcctgtcc gggataataa acctgattaa aaccgggcgc gggggtaatg gtatttaatt
     6301 cgcgcatggt ctggctaaga tgttgacgga gtaattcgct ggaggagaaa aagttcgggt
     6361 taataactat atgtaattgc cccaaattac gcccggcgtg taaatcgtca tacatcgaac
     6421 taacctgtcg cccgaacggt aagccgagta agacgcctga gaggacgtca atcatcatca
     6481 tcaggccata ccctttcggc ccagcggcgg ggagcagagc atgtaccgcg aacggatcgg
     6541 ttgttggtgc accgttttta tcgactgccc aggtatccgg gatagacata ttgcgcgagc
     6601 gggcgtcgag cacttttccc catgcctgta cggtagtcgc catatcgaag gtaaggatct
     6661 catcgccttc tcccggcgcg gcaaaggcca gtgggttagt gccgtagtaa atttccgcgc
     6721 cgccaaacgg caccaccatt ggatcggact ggcacagcga aatgccaatc aatccggcgc
     6781 gggcagcctg ctgcacaaaa taagagattg cgccgctgtg acccatccgg ctgataccga
     6841 ccaccgcaac gcctttttgc tgggcggttt tgatggcatg ttccataccc attttcgccg
     6901 cgacctgtcc ggcggcattg tcggcatgta aaattgccga gcacggtccg gtttcctcaa
     6961 gacgaaactc cggttcgcgg ttggtgccgc cttttgaaat gcgttcggcg tagtattcaa
     7021 cgcgcaccgc gccatgagag tggatccctc tggcatcggc gtaaaccaat acttcagcca
     7081 cggttgcagc gtgctcacgt tttaacccag cctggcagag tttattctcg attagctggt
     7141 ggagtgtttc ccgactgatt ttcatctgtc ttccttttta acgacggtgt gaagcatgac
     7201 tgcaattaac atacagggaa aatatctgga ttatgtgatc cagacaggca aaaaaatata
     7261 gttagaattt atttgataat cttcgtgctt ttaacctggt ttttaaaaca accatgctta
     7321 ttaaaaaata acgcgtaata gtctggcggt tatttgaatt cttttgttaa taatccacgt
     7381 gtgatattca tcaccttatt tactcgttgt catcgatacc gtaatcgcca cattaacact
     7441 gctcgtgcaa ttgccatggg tgcaattttt aaggagttgt tatgatccac gcctttatta
     7501 aaaaagggtg ttttcaggat tcggtcagtt taatgattat ttcacgaaaa ctcagcgaat
     7561 cagaaaatgt tgatgatgtt tccgtaatga tgggtacgcc cgccaataaa gcgttattag
     7621 atactacagg tttctggcat gacgatttta ataatgccac gccgaacgat atttgcgtgg
     7681 caattcgtag cgaagcggcg gatgcgggga tcgcgcaggc ggttatgcag cagcttgaag
     7741 aggcgctaaa acaactggcg cagggttcag gcagcagcca ggcgttgacg caggtgcgtc
     7801 gctgggacag tgcctgtcag aaattacccg atgccaatct ggcgctgatt tcagtggctg
     7861 gtgagtatgc ggcggagctg gcgaaccagg cgctggatcg caacctcaac gtgatgatgt
     7921 tttccgataa cgtcacgctg gaagatgaaa tccaacttaa aacccgcgcg cgggaaaaag
     7981 gcttgctggt gatggggcca gactgcggta cgtcgatgat tgccgccacg ccgctggctt
     8041 ttgctaacgt gatgccggaa ggcaacattg gcgtcatagg cgcttccggt accgggattc
     8101 aggagctgtg ctcacagatt gcgctggcag gggagggaat tactcacgcg attggtcttg
     8161 gcgggcgcga tctcagccgt gaagtgggcg gcatcagtgc gctaacggcg ctggaaatgc
     8221 tcagtgcaga cgagaaaagc gaagtgctgg catttgtttc aaaaccacct gccgaaactg
     8281 tgcgtctgaa aattgttaat gccatgaaag caactggcaa accgacggtg gcgctgtttt
     8341 taggttatac cccggcggtg gcccgcgacg agaatgtctg gtttgcctcc tcgctggatg
     8401 aggccgctcg tctggcttgt ctgctttcac gcgtcactgc gcgacgtaac gcgatagcgc
     8461 ctgtcagcag cggatttatt tgcggtttgt ataccggcgg tacgctggct gctgaagcgg
     8521 cgggattact tgccggacac cttggcgtgg aagccgacga tacccatcaa catggcatga
     8581 tgctggacgc cgacggacat cagatcctcg acctgggcga tgatttttac accgtcgggc
     8641 gtccccatcc gatgatcgac ccgaccttac gcaacctgtt aattgccgat ctcggcgcta
     8701 aaccgcaagt gcgcgtgttg ctgcttgatg tcgtgattgg cttcggtgcg accgccgatc
     8761 ctgccgcctc gctggtgagc gcctggcaaa aagcctgtgc cgcgcgttca gacaatcaac
     8821 cactgtatgc cattgccacg gtgacaggca ctgaacgtga cccgcaatgc cgctcgcagc
     8881 aaatcgccac gctggaagat gcggggatcg cggtagtgag ttcgctaccg gaagccacct
     8941 tgctggctgc ggcgttaatt catccgctct cgtcagccac acagcaacac acaccgtcat
     9001 tactggaaaa cgtcgccgtg attaacatcg gattacgcag ctttgcgctg gagctacaaa
     9061 gcgccagcaa accggttgtg cattaccaat ggtcgccagt cgccggcggc aataaaaaac
     9121 tggctcgttt attagaacgt ttgcaataag gggttcccat gtttacatca gtggcgcaag
     9181 ccaatgctgc ggttatcgaa caaattcgtc gcgctcgtcc acactggctg gatgtgcaac
     9241 cggcttcttc acttatcagc gaactaaaca agggcaaaac actgcttcac gccgggccgc
     9301 caatgcgctg gcaggagatg accggaccca tgaaaggggc gtgcgtgggc gcatgtctgt
     9361 tcgaaggctg ggcgaaagat gaagcgcagg cgctggcaat actggagcag ggggaagtga
     9421 acttcattcc ttgtcaccat gtgaatgccg tcgggccaat gggtggcatt acttctgcca
     9481 gtatgccgat gctggtggtc gagaacgtga ccgacggcaa ccgggcgtac tgcaacctca
     9541 acgaaggtat cggcaaagtg atgcgttttg gcgcttacgg cgaagatgtc ctgactcgcc
     9601 atcgctggat gcgcgatgtg ttaatgccag tattaagcgc ggcgctgggg cgcatggagc
     9661 gcggtatcga tctcacggca atgatggcgc agggcattac catgggcgat gagttccatc
     9721 aacgcaatat tgcttcctct gcactgttaa tgcgtgcgct ggccccacaa attgctcgcc
     9781 tcgatcatga taaacagcac atcgccgaag tgatggattt cctcagcgtg accgatcagt
     9841 tcttcctcaa tctcgcgatg gcctactgca aggcggcgat ggatgcaggc gcgatgatcc
     9901 gcgcaggcag catcgttacg gctatgaccc gcaacggcaa tatgttcggg attcgggtaa
     9961 gcgggctggg tgaacgctgg tttactgcgc ctgtaaacac tccgcaaggt ctgtttttca
    10021 ccggcttctc gcaggagcag gcgaacccgg atatgggcga tagcgcgatt accgaaacct
    10081 ttggtatcgg aggtgcggca atgatcgccg cacctggcgt aacgcgcttt gtcggtgcgg
    10141 gtggcatgga agcggccaga gcggtatctg aagagatggc ggaaatttac cttgaacgca
    10201 atatgcaatt gcagatcccc ggctgggatt tccagggggc gagcctgggg ctggacattc
    10261 gtcgcgtggt agaaaccggc attacgccac tcatcaatac cggtatcgcc cataaagagg
    10321 cggggatcgg gcagattggc gcaggcaccg tgcgggcacc gctggcgtgc tttgaacagg
    10381 cgctggaagc actggctgaa agcatgggga ttggttgagg aacgcacaat gacgatcatc
    10441 catcctctac ttgccagccg tagcgcaccg aattatcgcc agtcctggcg gttagcggga
    10501 gtgtggcggc gggcgattaa cctgatgacg gaaagcggcg aactgttaac gctgcatcgt
    10561 caggggagtg gtttcggccc cggcggatgg gtgcttcgcc gcgcgcaatt cgatgcgtta
    10621 tgcggtggat tatgcggcaa tgaacgacca caggttgtgg cacaagggat tcgcctcggg
    10681 cgtttcacgg ttaaacaacc acagcgttat tatctgctgc gtattacgcc gcctgcgcat
    10741 cctcaacagc ttgccgttgc atggatgcaa cgcgcggagg aaaccggact tttcgggcca
    10801 ctggcattgg cggcaagcga tcctctgcct gctgaattac gccagtttcg tcactgtttt
    10861 caggccgcgc tcaatggcgt taagaccgac tggcggcact ggctgggtaa aggccccgga
    10921 ttaacgccaa gtcatgatga cacgctgagc ggaatgctgc tggcggcctg gtattatggc
    10981 gctttagatg cgcgctccgg tcgcccgttt tttgcctgtt ccgacaatct tcaactcgtt
    11041 accactgcgg tgagcgtcag ttatttacgt tatgccgcgc aaggatattt cgcctcgcca
    11101 ctcctgcact ttgttcatgc tctgagttgc ccgaaacgta ccgcagcggc gatcgattcg
    11161 ctgctggcgc tggggcatac gtcaggggca gatacgctgc tggggttctg gcttggccaa
    11221 caattattac aagggaaacc atgaaaacac tggttgtggc tcttgggggc aacgccttac
    11281 tccagcgcgg tgaggcgctg acggcagaaa atcaatatcg caatattgcc agtgctgtgc
    11341 ccgcgctggc gcgcctggcc cgttcttatc ggctggcgat tgttcacggc aacgggccgc
    11401 aggtggggct gctggcgtta cagaatctgg cgtggaaaga ggtagaaccg tatccgctgg
    11461 atgtactggt ggcggaaagc caggggatga ttggctatat gctggcgcag agcttgagcg
    11521 cacagccgca gatgccgcat gtcacgacgg tgctgacgcg cattgaggtt tcgcctgatg
    11581 atccggcgtt tttgcagcca gagaagttta ttggtccggt ttatcagcca gaagaacaag
    11641 aggcactgga agcgacttac ggctggcaga tgaaacgtga tggtaaatat ttgcgccggg
    11701 tggtggcgtc tccgcaaccg cgtaaaattc tcgacagcga agccatcgag ttgttgctca
    11761 aagaggggca tgtggtgatt tgcagtggcg gcggcggtgt gcctgtgacg gaagacgggg
    11821 cagggagtga agcggtgatt gataaagatc tcgccgctgc gttgctcgcc gagcagatta
    11881 atgccgatgg actggtgatc ctcaccgatg ctgatgcggt atatgaaaac tggggaacgc
    11941 cgcagcaacg tgccattcgc catgccacac cggatgagtt agcgccattt gccaaagccg
    12001 atggttcgat ggggccgaag gtaacggcgg tgagtggcta tgtcagaagc cgtggtaaac
    12061 ccgcgtggat tggggcgtta tcgcggattg aagagacgct ggcgggcgaa gcggggactt
    12121 gtatttcgtt gtagtgatgg gcaccaggca attggcacat ccagcctgat gcgatgctgg
    12181 cgcgtcttat caggtctaca gtcggtgttg gagcggtggg ccggataagg cgtttacgcc
    12241 gcgtccggca atcggcacac catgcctgat gcgatg
//
LOCUS       DAZFFE010000120        11962 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFFE010000120 DAZFFE010000000
VERSION     DAZFFE010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.120"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..342
                     /locus_tag="ACHJ69_003982"
     CDS             <1..342
                     /locus_tag="ACHJ69_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016865.1"
                     /translation="KEHHYTQHELSPFFLYTPESLRNLPNISNVAEYSYYYNVDDMQT
                     RVIVTWRNIDNIFLQKAKLIDFLKRMGPSLQNDCIWFFHDKSDYANNFQRYCIIEHRD
                     SLQVEYFETIE"
     gene            497..1261
                     /gene="yabI"
                     /locus_tag="ACHJ69_003983"
     CDS             497..1261
                     /gene="yabI"
                     /locus_tag="ACHJ69_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO5016866.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWYAWLAGIIGCLMGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLFWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(1375..2073)
                     /gene="thiQ"
                     /locus_tag="ACHJ69_003984"
     CDS             complement(1375..2073)
                     /gene="thiQ"
                     /locus_tag="ACHJ69_003984"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO5016867.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQKKMHAIAHQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSSSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGIKG"
     gene            complement(2057..3667)
                     /gene="thiP"
                     /locus_tag="ACHJ69_003985"
     CDS             complement(2057..3667)
                     /gene="thiP"
                     /locus_tag="ACHJ69_003985"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO5016868.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWV
                     SVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRQMLLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLWQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTALIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKIPGRNVKTD"
     gene            complement(3643..4626)
                     /gene="thiB"
                     /locus_tag="ACHJ69_003986"
     CDS             complement(3643..4626)
                     /gene="thiB"
                     /locus_tag="ACHJ69_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO5016869.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEDKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     AEAVNVPGGWNNDTFVPYDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYEDPRT
                     STPGLGLLLWMQKVYGDNAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAATNFIEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(4657..4756)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(4790..6445)
                     /gene="sgrR"
                     /locus_tag="ACHJ69_003987"
     CDS             complement(4790..6445)
                     /gene="sgrR"
                     /locus_tag="ACHJ69_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO5016870.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQETTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     NIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQAGAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     gene            6534..6665
                     /gene="sgrT"
                     /locus_tag="ACHJ69_003988"
     CDS             6534..6665
                     /gene="sgrT"
                     /locus_tag="ACHJ69_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO5016871.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(6773..7378)
                     /gene="leuD"
                     /locus_tag="ACHJ69_003989"
     CDS             complement(6773..7378)
                     /gene="leuD"
                     /locus_tag="ACHJ69_003989"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO5016872.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(7389..8789)
                     /gene="leuC"
                     /locus_tag="ACHJ69_003990"
     CDS             complement(7389..8789)
                     /gene="leuC"
                     /locus_tag="ACHJ69_003990"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO5016873.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(8792..9883)
                     /gene="leuB"
                     /locus_tag="ACHJ69_003991"
     CDS             complement(8792..9883)
                     /gene="leuB"
                     /locus_tag="ACHJ69_003991"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO5016874.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPTATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELTHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(9883..11454)
                     /gene="leuA"
                     /locus_tag="ACHJ69_003992"
     CDS             complement(9883..11454)
                     /gene="leuA"
                     /locus_tag="ACHJ69_003992"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO5016875.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(11546..11632)
                     /gene="leuL"
                     /locus_tag="ACHJ69_003993"
     CDS             complement(11546..11632)
                     /gene="leuL"
                     /locus_tag="ACHJ69_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO5016876.1"
                     /translation="MTHIVRFIGLLLLNAYFLRGRRVSGIQH"
BASE COUNT         2686 a         3179 c         3200 g         2897 t
ORIGIN      
        1 aaggaacatc actatacaca gcatgaactt tccccatttt tcctttatac gccagagagt
       61 ttgcgaaatt tgccaaacat atcaaacgta gccgagtata gctattacta taatgttgat
      121 gatatgcaaa caagagtgat tgttacatgg cgaaatatag ataacatttt tcttcagaaa
      181 gcaaagctaa tagatttttt gaaaagaatg ggaccgagtc tacaaaacga ttgtatatgg
      241 ttttttcatg ataaaagtga ttatgcaaat aatttccagc gttattgcat cattgagcac
      301 cgtgattctc tgcaggtgga atattttgag actatcgaat gataggcaga gggtgtcttt
      361 tgtgccggtt gtgaagaaaa agtgaatgat gtagccgcca agttgtcata attggtaacg
      421 aatcagataa ttgacggctt gacggagtag catagggttt gcagaatccc tgcttcgtcc
      481 atttgacagg cacattatgc aagcattgct ggaacacttt attacccaat ccaccgtgta
      541 ttcattgatg gcggtggtgt tggtggcctt tctggagtcg ctggcgctgg tcggtttgat
      601 tctgcccggt acggtgctga tggcggggct gggagcgctg attggtagcg gtgagttaag
      661 tttctggtac gcctggctgg cggggattat tggctgcctg atgggcgact ggatctcctt
      721 ctggctgggc tggcgtttta aaaagccgtt gcatcgctgg tcatttctga agaagaacaa
      781 agcattactc gataaaaccg aacatgcgct gcatcagcac agcatgttca ccattctggt
      841 cggtcgtttt gtcggcccga cgcgtccgct ggtgccaatg gtggcgggaa tgctggatct
      901 cccggtggca aaatttatta cgccgaatat tatcggctgc ttgctgtggc caccgtttta
      961 cttcctgccg gggattctgg cgggtgcggc gatcgatatt cctgccggga tgcagagcgg
     1021 tgagtttaaa tggttactgc tggcaacagc ggtgtttttg tgggttggtg gctggctgtt
     1081 ctggcggtta tggcgcagcg gtaaagcgac tgaccgtttg agtcattatt tgtcccgcgg
     1141 tcgtttgttg tggctgacgc cgttgatttc tgccatcggc gtggtggcgc tggtggtgtt
     1201 aattcgccac ccgttgatgc cggtatatat cgatattttg cggaaagtgg ttggggttta
     1261 ggggatagcc ttgtgcgggt tgcctgatgc gacgcttgcc gcgtcttatc aggcctgcaa
     1321 aacgcactac ccgtaggtcg gataaggcgt tcacgccgca tccgacagtg catattaacc
     1381 tttaattccc aacagcgccg aagcactcgc cttaccgctc aacaactcat cggtcttacc
     1441 ctgccaggcg atacgcccgt cggcgaccac caccgagcgc gtggcgatcc gcgccgcatc
     1501 ttccacactg tgcgacacca tcaatagcgt cattttttgc tgttggcagc tcgaactcac
     1561 cagcgtcagc atctcctgac gtagcgccgg atcgagcgcg gaaaacggtt catcgagcaa
     1621 taaaatgggc tgttcgcgta ccagacaacg cgctaacgcc acgcgctgtc gttgaccgcc
     1681 ggaaagctcg ccaggtaacc gcgccattaa attatcaatc cccatctggt gggcaatcgc
     1741 gtgcattttc ttctgctgcg ccgcatttag tttcaacccc ggatttagcc ccagcccgat
     1801 gttctgcgcg accgtcaggt ggctgaacag gttgttctcc tgaaacagca tcgacaccgg
     1861 acggcgtgac ggcggcgtcg tggtgtgatc tacgccgtcg atagtcagcg aaccgctggc
     1921 tggcgtcaga aaaccggcga tcaaattaag cagggtactt ttacccgcgc cgcttggccc
     1981 gaggatcgcc acctgctcgc cgcgttccac cgttaagcta aaacgcatcg gcaaatggtg
     2041 gtaaagccag gtgatatcag tcagttttaa catttcgccc cggtattttt tcaatcacgg
     2101 taaacagcag aaaacagagc agcagcagaa ttaacgcggt gaccgcgccg tcctggctgc
     2161 gataggagcc aatttgctgg tagagataaa acggcagggt acggaaatca tcgttaccga
     2221 acaatgccac cacgccaaaa tcaccaatcg acagcacaca agcaaaggcc agcgcctgcg
     2281 ccagtggacg tttcagggcg cgcagctcca ccacttttaa gcgtgaccag ccttcaatgc
     2341 ccagcgactg acacaacatg ctgtagcggg cggtgatatc gcgcatcggg ttttccagta
     2401 ctttcagcgc ataagggatt gccattaacg cattggtgaa aatcacaatg ccgtcagcag
     2461 attgcggcag gccgatagtg ttgttgagca gtaaaaagaa gcccgtagcc agcacaatcc
     2521 ccggcatggc gaggatcaac atgccgctca tctccagcgc ctgacccgcc agcattttct
     2581 gccgcgcccg aagttcgcga ctgctccata gcagcatcat ggtcagcact acgcacaata
     2641 cgcctgccgc cagcgcaata cgcaacgagg tccacagcgc ctgccacagc accggttgtg
     2701 ccagcacttc cggcaactgg cgatttaccc catcgacgat caccgccagt aacggtggca
     2761 gcaacagcag cagcgccagc acaattaacg ccgtgtcgca aatgcgacta tgcagacgat
     2821 cgtccgggtc gcgccagcct tgcagcagtg tggtaccagg cgcaatggcc ttactcaatc
     2881 gctgactcaa cagcaccagc ccgaggcagc acaccatctg gatcagcgcc agcattgccg
     2941 cgcgggcagg atcgtagtcg taactcagtg cctgatagat tgccagctcg atagtggtcg
     3001 cctgcggacc gccgcccagc gatagcacgg tggcgaagct ggcgaaacag agcataaaga
     3061 ttagtgcggc aaccggcggg atttgtcgcc gtaaccacgg ccattcgacg aaacggaaaa
     3121 aatgccagcc gcgcatccca agttgggcgg caagctgacg ttgttcgccg ggaatgtttt
     3181 ccagtgcctg gagtaataaa cggctagcca tcggcagatt aaaaaacaca tgggccagca
     3241 aaatgccttg caggccgtag ggcgaaaagg tccactccag accaagtgat tgccagagcg
     3301 aggccagcca gccctggcga ccatagacgc taagaatgcc gaaaacggca accagcaccg
     3361 ggaggatcaa ggtcattgcg cacagacgca acagcatctg tcgcccagga aagcgcctgc
     3421 gatagagggc gcgggcgagg aatattgccg gtacgacaga gagcagcgcc gagagaaacg
     3481 cctgccagaa ggagaagcgc accacatgcc acaggtagct gtcctgccag actgacaccc
     3541 agtcaccctg cggcgcgttc caccacaggg cgagaaacgc cgccagtgca accgctacca
     3601 ccagcgtggc ggcgcttacg cctggaatta accagccggg aattaacggc tgacggcgcg
     3661 ttgccattcg ctaatccatg cctgacgttg tgccgccact tcggcgggtg tgaactctag
     3721 tgtggtggct ggtttggtca attgttcaaa tcctgcaggc agagtgacgt ttgctaccgg
     3781 atacatccag ttgccggttg ggatcgcatt ctgaaaagcc ggagaaacca taaactggag
     3841 gaatttttgc gccagctctg gctgcttgct ggcagcggtg cgggcggcaa cttccacctg
     3901 cagatagtga ccttcgataa agtttgtggc ggcgtagtta tctttcttct cttcgagaat
     3961 gtgataagcc ggagaggtgg tgtaactcag taccagatcg ctttcgcctt ttaaaaacag
     4021 gccgtaggct tcgctccagc ctttggtgac cgtgacagtt ttcttcgcca gtttctgcca
     4081 ggcttgcggg gcgttatcgc catagacttt ttgcatccat agcaacagac ccagccccgg
     4141 tgtactggta cgtggatctt cataaatcac ccgccagttt tgatcgctct caaccagttc
     4201 tttcaggctt tgcggcggat ttttcagttt gttcttgtca tagacgaagg cgaaatagcc
     4261 gtaatcatac ggtacgaaag tgtcattatt ccagccgcca ggaacgttaa cggcttccgc
     4321 tgccacaccg cttttagcaa acagccctgt tttgctggcg gcgtctaaca gattgttatc
     4381 cagccccagc accacatcgg ctttgctgtt tttgtcttcc atccgtagac ggttgagcag
     4441 cgaaacgcca tcttccagcg ccaccagttt cagttcgcaa ttacagtctg cttcaaaggc
     4501 ttttttgact accggaccag gtccccagtc agcggcaaag gaatcgtagg tataaacagt
     4561 tagaacaggt ttagcgaaaa caggcgctgt gcacagcaac agcaggggaa gacatttttt
     4621 taacactttg cacctcacaa aagagtggca aaggacttga gaaggagcct caaatccctt
     4681 cgccggcgtt atccggatca ggttcgacgg gtattttctc agcgcacgcg gctgcgtggc
     4741 accccgttga gaacggcgtt agtgtagtga ttttgttatc aaccagcaat catggatccg
     4801 gtggtgcaaa ccacgctgat ttaaaatcga accagccgag ggtgttcatg cgcaggccgc
     4861 gcatactacg ttgcccctga atgatcagcc agtggtggat aagtggcacc attgctttgc
     4921 tggcgaccag ttgctggcac cagttcgcca ggttcatttc gccattgcgc cagcgggcgg
     4981 cgccggcttg ccagtcgatc ggaatacagt gttggagcag cggcacctcg cacaagtgcg
     5041 cgaacagcga aaaatccagc ggcagcgtaa agttggcgct gttaagccag atatcgctct
     5101 cgatctctcc ttcatgccac tgatcgtagc tgatctcttt gatttccagc gtgacctggt
     5161 gacttgccag aatctgctgc atgatcccgg caatcacccg atgctcgctg tgatcctgat
     5221 aaaaggtcag ggtgaggctt tccagaccag ccggtttttc gctctttatg gtgcgggcat
     5281 ggtgccaacg ggggagcagt ccataagccg ggaaccacag ttgctggtac tgttcctcag
     5341 cgaaatagac cagattcgtc ggagaaagca catagcttac ccagtctctg acttgctgat
     5401 tcgcccctcg atgggtgcgg ctgtcgaaca gtaaatagta gcaaccttcc tccaggcggc
     5461 tttcaatctc tttttcctcg ccctgtggac cttttagcat cagccctccg gctggctcgt
     5521 cggcaatttc cggcagaacc cagacgttaa cttcgtcgat taatgcccgg taaccgaaga
     5581 agtcatcgaa tgcctgaatt ttcagttgat tggtgctgtt gcggatcacc gcatacggac
     5641 cggtgccgat gggatggctg gcaaagttac tgagggtttc ccattcgcgc ggcaggatca
     5701 ttgccggaac ttgccccagc agtaacggca accagcggtc cggttgcgtg agatggatat
     5761 ccagcgtcca gggcgtcggc gacacaatgt tagcaatatg cgaatagagc ggaagcgtat
     5821 tgattcgttt taaagaggcg atcacatcgt ccatttccag ttcacgacca tggtgaaaat
     5881 ggactcctgg acgcaaaaag aaacgccagt gaagcggtga tatttgctgc cagtggtgtg
     5941 cgatgtctgc ttccagttcc ccattttcct catttatgcg cgttagcgaa ctgaagattt
     6001 gccgggcgat atgggtttca gaacggcgca atgcgctgcc aggtagcaga ttacgcaacg
     6061 gacgatagta gagcacgcgc aggatgtgcc gcccctggcg gaagctgcgg cccagatgag
     6121 aaaccagcat ttgccgcaca gtcgctttgt cgccaaccaa ctgcactagt tggtcgatac
     6181 gatcctgctc cagcaggtct tccgcccgct gttgctgaag cgccagcccg gtatagagga
     6241 atgtcaggcg tgaacgttta ccgcgcccga cttccgcttc ccacgtcagc cagccgcgat
     6301 cctgcatggt gttgagcagg gtgcgcatat gacgacgcga gcaactcagt aacgctgcca
     6361 gttcgttgag cgtggtctcc tgagatttac cctcgcagca ttgccacagg cggatgaact
     6421 gttgttgcag acgagcagat ggcataaaag gggaactcct gcgcaaaaga cagcaatttt
     6481 attttcccta tattaagtca ataattccta acgatgaagc aaggaggtgc cccatgcgtc
     6541 agttttatca gcactatttt accgcgacag cgaagttgtg ctggttgcgt tggttaagcg
     6601 tcccacaacg attaaccatg cttgaagagc tgatgcagtg ggaggggagt cattctgact
     6661 actgatttgc ggacatcatg tgtgactgag tattggtgtt aatcgccacg ccagcagtga
     6721 ttatctgctg gcttttttct ttttggcgat tgagtgccgg acggtgtaca aactaacgca
     6781 taaacgccgg ttgcttctct tcataagacg caatagcatc gtcgtgctgc agagtcaggc
     6841 cgatgctgtc cagaccgttc atcatgcagt ggcggcggaa ggcatcgatg gtaaagcgat
     6901 aagttttctc tcccgctttc acctcttgcg cttccaggtc tacatcaaaa tgaatccccg
     6961 gattggcttt caccagcgca aacagctcat ccacttctgc atcgcttaat ttcaccggca
     7021 gcagctggtt gttaaagcta ttgccgtaga agatgtcagc aaaactcggc gcaatcacca
     7081 ccttaaaacc gtaatcggtc aatgcccacg gcgcgtgctc acgcgaggaa ccgcagccga
     7141 agttttctcg tgccagcaaa atggaagcgc cctgatactg cgggaagttc agcacgaagt
     7201 ccgggtttgg ctgttggcct ttttcatcca gaaaacgcca gtcgttaaac agatgcgcgc
     7261 caaaacccgt acgggtcact ttctgcaaaa actgtttcgg gatgattgca tcggtatcga
     7321 cattggcggc atccaacgga accaccaggc ctgtgtgttt gataaatttc tctgccatgg
     7381 tgtgctcctt atttaatgtt gcgaatgtcg gcgaaatgtc cggtcacagc cgcggcggca
     7441 gccattgccg gactgaccag atgcgtgcgc ccgccgcgcc cctggcggcc ttcaaagtta
     7501 cggttgctgg tggaggcgca acgttcgccc ggattcaggc ggtcgttgtt catcgccagg
     7561 cacattgaac aacctggcaa gcgccattca aaaccagctt cgataaaaat tttgtccaga
     7621 ccttccgctt ccgcctgggc tttaaccgga ccggagccgg gaaccaccag cgcttgtacg
     7681 cctggcgcga ctttccggcc tttggcgatt tccgctgccg cgcgtaaatc ttcaatacgc
     7741 gagttagtac aggaaccaat aaacactttg tcgatagcca cttctgtcag cggaataccc
     7801 ggtttcagcc ccatataggc cagcgctttt tctgccgacg cgcgttcaac cggatcggca
     7861 aacgaagccg gatcgggaat attgtcgttc acagaaatca cctggcctgg attggtgccc
     7921 caggtgacct gcggcgaaat ctcttctgct tgcagagtga caacggtatc gaaagttgct
     7981 ccttcgtcgg tttgcagggt tttccagtag gcaacggcgt cgtcgaaatc tttgcctttc
     8041 ggcgcatgca gacggccttt aacatagtta aaggtggttt cgtccggtgc aaccagaccg
     8101 gcttttgcgc ccatttcgat tgccatattg cacagggtca tacgaccttc catgcttaaa
     8161 tcacggattg cttcgccgca aaattccacc acatgcccgg taccgcctgc gctgccggtt
     8221 ttaccaataa ttgccagcac gatatctttt gcggtaatgc ccggcgcggc tttgccctgg
     8281 acttcaattt tcatggtctt cgcccggccc tgtttcaggg tttgcgttgc cagtacgtgt
     8341 tcaacttcgg aagtgccgat accaaaggcc agtgcgccaa acgcgccgtg ggtggcggta
     8401 tgtgagtcgc cgcagacaat ggtcatcccc ggcaaggtca cgccctgttc cggccccatt
     8461 acgtggacga tcccctgata cgggtgattc aggtcataca gctcgacgcc aaattctttg
     8521 cagtttttga tcagctcctg catctggatg cgcgccattt caccgcaggc attaatgtct
     8581 ttcgtctgag tagagacgtt gtgatccatg gtggcgaagg ttttgcccgg ctgacgtacc
     8641 gggcgaccgt gggcgcgcag accatcgaac gcctgcggtg aggtcacttc atgcaccagg
     8701 tggcggtcga tatataacag cggggtttcg ttttcggctt cgtacactac gtgagcgtcg
     8761 aataattttt cgtataacgt cttagccatg attacacccc ttctgccaca tagcgggcaa
     8821 tgatatcgcc catttcatcg gtactaacgg cggcagcgcc acgggctaaa tccccagtgc
     8881 gaatgccttc ttctaatgcg cggttaatgg cgcgttcaat ggcggaagcc gcatcatcgg
     8941 catccaggct gtaacgcagc agcagcgcca gcgacagaat ttgtgcaatc gggttggcga
     9001 tatttttacc tgcgatatcg ggtgccgagc cgcccgccgg ttcatacaga ccaaaacctt
     9061 gctcgttcag gctggcggaa ggcaacatcc ccattgagcc agtgatcatt gcgcactcat
     9121 cagacagaat gtcgccaaac aggttggagc acagcaagac gtcaaactgt gatggatctt
     9181 taatcagctg catggtggcg ttgtcgatgt acatatgcgt cagttcgacg tccgggtatt
     9241 ccgtggcgat ctcgttaacg atctcccgcc ataaaataga ggattgcagc acgttggctt
     9301 tgtcgattga ggtgactttg tggcgacgct tgcgggcaga ttcaaatgcg atgcgggcaa
     9361 tacgctcgat ctcaaaacgg tggtacacct cggtatcaaa cgctttttca tattgtccgc
     9421 taccttcgcg gccttttggc tgaccgaaat agatgccgcc ggtcagttcg cgcacacaca
     9481 ggatgtcaaa gccgtttgcg gcaatgtcag cacgcagtgg gcagaacgct tccagcccct
     9541 gatacagttt tgccggacgc agattgctga ataatttgaa gtgtttacgc aaaggcagca
     9601 gtgcgccgcg ttctggttgc tggtctggcg gcaaatgttc ccacttcgga ccacctaccg
     9661 agccaaacag cacggcatcg gcttgctcac aaccttcaac cgtcgcagtc ggcagcggct
     9721 gtccgtggtt atcaatggct gcgccgccta catcgtaatg gctggtggtg atacgcatcg
     9781 caaatcggtt gcgcacggca tccagcactt tcagcgcctg ggtcatcact tccggaccaa
     9841 taccgtcccc cggcaatacg gcaatatggt aattcttcga catcacacgg tttccttgtt
     9901 gttttcgttg tgttgagctt tgcgttgcaa ctctttttcg acttctgcgg cacgccagat
     9961 attgttcagt acgtgcacca tggctttggc ggaggactcg acaatatcgg tggccaggcc
    10021 gacgccgtgg aagcggcgac cgttgtagtt ggcgacaata tccacctgac ccagcgcatc
    10081 tttaccgtgg cctttggcgg tcaggctgta tttcaccagt tcgacgttat agtcagtgat
    10141 gcggtttatc gcctggtaga cggcatcgac cggaccgtta ccgttggcgg cttctgcttt
    10201 gacttcttcg ccacaggcca gtttgacggc ggcagtggca atatcgttag aacctgactg
    10261 cacgctgaag taatccagac ggaaatgctc cggctcttct tgctgcttac cgatgaaggc
    10321 cagcgcctcc agatcgtaat caaacacctg gccttttttg tccgccagct tcaggaaagc
    10381 gtcgtacagg ttgtctaaat tatattcact ttctttatat cccatctcat ccatgcgatg
    10441 tttcaccgcc gcacgccccg aacgagaggt cagattcagc tggatttggt ttagaccaat
    10501 agattctggt gtcatgattt cgtagttttc gcggtttttc agtacaccat cctggtggat
    10561 accggaggag tgtgcgaatg cgccgctgcc aacaatggct ttgtttgccg ggatcggcat
    10621 attacaaatc tggctaacta actggctggt gcgccatatc tcctggtgat tgatggcggt
    10681 gtggacgttg agaatatcct tacgaacttt gatcgccatg atgacttctt caagggaaca
    10741 gttaccggca cgctcgccga tcccgttcat tgcgccttct acctgccgtg ctccggcatg
    10801 taccgccgcc agtgagttgc cgaccgccag gcccaaatcg tcgtgggtat gtacggaaat
    10861 aatggctttg tcgatgttgg gtacgcgttc atacaggccg ctgatgattc cggcgaactc
    10921 aaatggcatg gtgtagccca cggtgtccgg aatgttgatg gtggtggcac cggcattaat
    10981 cgccgcttcg accactcgcg ccagatcggc aattggggtg cgtccggcat cttcgcaaga
    11041 aaattcaaca tcatcggtgt aattacgggc gcgtttcacc atatagatag cgcgttcgat
    11101 cacttcgtcc agcgtgctgc gcagcttggt ggcgatgtgc attggcgaag tggcaataaa
    11161 agtatggata cggaaggctt cggcgacttt cagggattcg gctgccacgt caatatcttt
    11221 ttccacgcag cgagctaacg cgcatacacg gctgttttta acctggcgag cgatggtctg
    11281 cactgattca aaatcacccg gcgaagagac ggggaaaccg acttccatca cgtcaacacc
    11341 catacgctca agggccagcg caatttgcag tttttctttc acactcaagc ttgcctgtaa
    11401 cgcctgttca ccgtcgcgca atgtggtatc gaaaataatg acttgctggc tcatggtttg
    11461 ggtccttgtc tctttttgag cgcctcgctt cgggcataaa aaaacccgcg caatggcgcg
    11521 ggtttttgtt tgactgcgtg ctggcttaat gctggatgcc gctcactcgt ctaccgcgca
    11581 aaaaatatgc gtttagtagt agtagaccga taaagcgaac gatgtgagtc attaaatcag
    11641 ctccagatga atgcgatatg cttttagagt tactggatac aaaaacggat gtcaaccctg
    11701 aagcaataaa aacgtcacgc cattgtgagt tcggcatccg taaaattagc taattgtgct
    11761 gcggtggtta aaacaagcga tattaatttc tgcttaaccg ccgatgcttt tcatcggttg
    11821 acatatttca gaataatttt ttgcatctaa tcaacgaggg aaagggggat aaaatgcacg
    11881 cgttacaaaa cctatcctga tgatttgtat tgaattatat gttttgcgaa ttttttgata
    11941 ttcatttgct gaatattatt ga
//
LOCUS       DAZFFE010000121        11778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZFFE010000121 DAZFFE010000000
VERSION     DAZFFE010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.121"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..154)
                     /locus_tag="ACHJ69_003994"
     CDS             complement(<1..154)
                     /locus_tag="ACHJ69_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO5016877.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILI"
     gene            complement(269..580)
                     /locus_tag="ACHJ69_003995"
     CDS             complement(269..580)
                     /locus_tag="ACHJ69_003995"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO5016878.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHAIAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            779..1477
                     /locus_tag="ACHJ69_003996"
     CDS             779..1477
                     /locus_tag="ACHJ69_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5016879.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(1522..2421)
                     /gene="eamA"
                     /locus_tag="ACHJ69_003997"
     CDS             complement(1522..2421)
                     /gene="eamA"
                     /locus_tag="ACHJ69_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO5016880.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHATRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            2616..3803
                     /gene="ydeE"
                     /locus_tag="ACHJ69_003998"
     CDS             2616..3803
                     /gene="ydeE"
                     /locus_tag="ACHJ69_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO5016881.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMHSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIVVAWVLMLKGIRARPWGQP
                     ALC"
     gene            3930..4025
                     /gene="mgtS"
                     /locus_tag="ACHJ69_003999"
     CDS             3930..4025
                     /gene="mgtS"
                     /locus_tag="ACHJ69_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO5016882.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            4025..4129
                     /gene="mgtT"
                     /locus_tag="ACHJ69_004000"
     CDS             4025..4129
                     /gene="mgtT"
                     /locus_tag="ACHJ69_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO5016883.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(4244..5134)
                     /gene="dgcZ"
                     /locus_tag="ACHJ69_004001"
     CDS             complement(4244..5134)
                     /gene="dgcZ"
                     /locus_tag="ACHJ69_004001"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO5016884.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASEPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSSMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKATN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(5388..5492)
                     /locus_tag="ACHJ69_004002"
                     /pseudo
     CDS             complement(5388..5492)
                     /locus_tag="ACHJ69_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00156 family protein"
     gene            5516..5596
                     /locus_tag="ACHJ69_004003"
                     /pseudo
     CDS             5516..5596
                     /locus_tag="ACHJ69_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5641..7686)
                     /gene="dcp"
                     /locus_tag="ACHJ69_004004"
     CDS             complement(5641..7686)
                     /gene="dcp"
                     /locus_tag="ACHJ69_004004"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO5016885.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGIQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLEQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNNWL
                     IPLLNTTQQPALAELRDRATREKLFTAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATNPQVFARYARHYQSRAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGRRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            7823..8569
                     /gene="ydfG"
                     /locus_tag="ACHJ69_004005"
     CDS             7823..8569
                     /gene="ydfG"
                     /locus_tag="ACHJ69_004005"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO5016886.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            8658..9344
                     /gene="rspR"
                     /locus_tag="ACHJ69_004006"
                     /gene_synonym="ydfH"
     CDS             8658..9344
                     /gene="rspR"
                     /locus_tag="ACHJ69_004006"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO5016887.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            9521..9724
                     /gene="ydfZ"
                     /locus_tag="ACHJ69_004007"
     CDS             9521..9724
                     /gene="ydfZ"
                     /locus_tag="ACHJ69_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO5016888.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(9760..11220)
                     /gene="ydfI"
                     /locus_tag="ACHJ69_004008"
     CDS             complement(9760..11220)
                     /gene="ydfI"
                     /locus_tag="ACHJ69_004008"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO5016889.1"
                     /translation="MGNHLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMCDVVTSYAQAIDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQVP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGTARVQSLLAIKAIF
                     GDDLPGNSLFTTKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(11309..>11778)
                     /gene="ydfJ"
                     /locus_tag="ACHJ69_004009"
     CDS             complement(11309..>11778)
                     /gene="ydfJ"
                     /locus_tag="ACHJ69_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFO5016890.1"
                     /translation="SDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPSTIMVALIV
                     IHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGIFCTMTES
                     WYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPHVVRAAQPSRSL"
BASE COUNT         3143 a         2899 c         2618 g         3118 t
ORIGIN      
        1 taattaatat aattaatgag cttgccatga taattggaaa cgtaaaaacg aatccatctc
       61 tcaccgctgc aacatgtttt tgtgagttta acttgatagc aactggaaca agaaaacgtt
      121 ccattccacg ttcgaatgat gccattaatc ccattttttt atacctgttg tcctggatat
      181 ttatacatcc caataaagca tgtatgccat atccatttta tgatgtgtga aaagttagtt
      241 caggtaatag tttaataata ttagattctt actcttgcag tagtttcatc gcaaattcaa
      301 gaactgcctg tccgttcatt gtgccatagt ctttcatctc gataacggct acaggcattt
      361 tcccctgtgc aacggcttca atctctggtt tctgaaagcg aacttgcgga ccaagtaaaa
      421 caacgtcaac ttcgttattt ttaattttcc ctttagcctc tgcaatcgcc aatgcggaaa
      481 tattaacttc aagtgaaata gcgatagcat gatcaatcat acgtttaacc agcatactgg
      541 ttgacatgcc cgcagcgcaa actaacaata tctttttcat atttttcctt actggtatat
      601 aacagactac attgtgtaaa tatcatatcc cacagtagct acacgtcctg gtacattgat
      661 cacagtaata cagcaataaa gttgttcaac aacagggtga taattaaacg actcggccat
      721 acgataaact aatcaggtct caactccttg ttttcaatgg ctcttaaaag gtgcaaaagt
      781 gatttttcaa aagattgccc gcttacttaa atccgagatc aatggcaatt catggcatgt
      841 tggtgatttg ttgccgtcag aagcggaact cgctgttcgt tataatgttt cgcgtaatac
      901 cctgcgtaag gcattatccc tgctggaagg cgaaggaatt attcacagaa agcatggttc
      961 aggaacatac attcaaaaaa agaattttgt tgcacacatt gatcacatga acagtttcag
     1021 tgaaattgca cataaaagcg gcaaagaggc aggaagtcag attatgaaat ttgaagtgca
     1081 ggatgcttct cctactattg cgactgagct gaatttagtg actggtgagc aggtttatta
     1141 cataaagaga ctgcgattca ttgaggataa tgcagcacaa ttagaagaaa cgtggatgtc
     1201 agtggcacgt tttcctgatt taaccgtatc acatatgcaa aaatccaaat tttcgtatat
     1261 tgagaacgaa tgcgggatca aaatcattgg cacctttgaa actttctccc cgacttttcc
     1321 taccccagaa atcgccagta ttttacggat cagcccacgg gatcccatac ttaaaattca
     1381 gacccaggct gtggatagta actctattcc gctggattat tcgttacttt acagcaatat
     1441 tttcgagttc caggtaaagt acttttttcc gcgataaaag cagacgcaca gagtgctggc
     1501 atcacattct gtgcgtatgg tttagcctct gacctttacc gctttacgcc accgcaagcc
     1561 aaatacattg atatacagcc cggtcataat gagcaccgca ccgaaaaatt gcagacccgt
     1621 taagcgttca tccaacaata gtgccgcact tgccagtcca actacaggca ccagtaacga
     1681 taacggtgca acccgccagg tttcatagcg tcccagtaac gtcccccaga tcccataacc
     1741 aacaattgtc gccacaaacg ccagatacat cagagacaag atggtggtca tatcgatagt
     1801 aaccagactg tgaatcatgg ttgcggaacc atcgagaatc agcgaggcaa caaagaaggg
     1861 aatgattggg attaatgcgc tccagattac cagcgacatc actgccggac gcgttgcgtg
     1921 cgacatgatc tttttattga agatgttgcc acacgcccaa ctaaatgctg ccgccagggt
     1981 caacataaag ccgagcatcg ccacatgctg accgttcaga ctatcttcga ttaacaccag
     2041 tacgccaaaa atcgctaagg cgatccccgc caattgtttg ccatgtagtc gttcaccgaa
     2101 agtaaacgcg ccaagcacga tagtaaaaaa cgcttgtgcc tgtaacacca acgaggccag
     2161 tccagcaggc ataccaaagt taatagcgca gaacagaaag gcaaactgcg caaaactgat
     2221 ggttaatcca taccccagca gcaaattcag tggtactttc ggtcgtgcga caaaaaagat
     2281 ggccgggaaa gcaaccagca taaagcgcaa accggccagc atcagcggtg gcatgttatg
     2341 aagccccact ttgatgacca caaaatttag cccccataca accactacca gtagcgccaa
     2401 caccccatct tttcgcgaca ttctaccgcc tctgaatttc atcttttgta agcaatcaac
     2461 ttagctgaat ttacttttct ttaacagttg attcgttagt caccgtttac gacggcatta
     2521 atgcgcaaat aagtcgctat acttcggatt tttgccatgc tattccttta catctctaaa
     2581 acaaaacata acgaaacgca ctgccggaca gacaaatgaa cttatcccta cgacgctcta
     2641 ccagcgccct tcttgcctcg tcgttgttat taaccatcgg acgcggcgct acgctgccgt
     2701 ttatgaccat ttacttgagt cgccagtaca gcctgagtgt cgatctaatc ggttatgcga
     2761 tgacaattgc gctcactatt ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata
     2821 agttcgacaa gaaacgctat atgctactgg caattaccgc cttcgctagc ggttttattg
     2881 ccattccttt agtgaataac gtgacgctgg tggtgctctt ttttgccctt attaactgcg
     2941 cctactccgt ttttgctacc gtgctgaaag cctggtttgc cgacaatctt tcgtccacca
     3001 gcaaaacgaa gattttctca atcaactaca ccatgctcaa cattggctgg accatcggtc
     3061 cgccactcgg cacgctgttg gtgatgcata gcatcaatct gcccttctgg ctggcagcta
     3121 tctgttccgc gttccccatg cttttcattc aaatttgggt aaagcgcagc gagaaaatca
     3181 tcgccacgga aacaggcagt gtctggtcgc cgaaagtttt attacaagat aaagcactgt
     3241 tgtggtttac ctgctctggc tttctggctt cttttgttag cggcgcattt gcttcatgca
     3301 tttcacaata tgtgatggtg attgctgatg gtgattttgc cgaaaaggtg gtcgcggttg
     3361 ttcttccggt gaatgctgcc atggtggtta cgttgcaata ttccgtgggc cgccgcctta
     3421 acccggctaa catccgcgcg ctgatgacag caggcaccct ctgtttcgtc atcgggctgg
     3481 tcggttttat tttttccggc aacagcctgc tattgtgggg tatgtcagct gcggtattta
     3541 ctgtcggtga aatcatttat gcgccgggcg agtatatgtt gattgaccat attgcgccgc
     3601 caggaatgaa agccagctat ttttccgccc agtctttagg ctggcttggt gctgcgatta
     3661 acccattagt gagtggcata gtacttacca gtctgccgcc tttctcgctg tttatcatct
     3721 tagcgttggt gatcgttgtt gcgtgggtgc tgatgttaaa aggaattcgt gcaagaccgt
     3781 gggggcagcc cgcgctttgt tgatttaagt cgaacacaat aagaatttaa ttcagccttc
     3841 gtttaggtta cctctgctaa tatctttctc attgagatga aaattaaggt aagcgaggaa
     3901 acacaccaca ccataaacgg aggcaaataa tgctgggtaa tatgaatgtt tttatggccg
     3961 tactgggaat aattttattt tctgggtttc tggccgcgta tttcagccac aaatgggatg
     4021 actaatgaac ggagataatc cctcacctaa ccggcccctt gttacagttg tgtacaaggg
     4081 gcctgatttt tatgacggcg aaaaaaaacc gccagtaaac cggcggtgaa tgcttgcatg
     4141 gatagatttg tgttttgctt ttacgctaac aggcattttc ctgcactgat aacggatcgt
     4201 tgacacagta gcatcagttt tctcaatgaa tgttaaacgg agcttaaact cggttaatca
     4261 cattttgttc gtcaataaac atgcagcgat tccttccggt ttgcttaccc tcatacattg
     4321 ccctgtccgc tcttccaatg accacatcca gaggctcttc aggaaatgca cgactcacac
     4381 cagcagtcac ggtaatgttg atatgccctt cagaatgtgt gatggcatgg ttatcgacta
     4441 actggcaaat tctgacacct gcacgacatg cttcttcatc attagtcgct ttgacaataa
     4501 tgataaattc ttcgcccccg tagcgataaa ccgtttcgta atcgcgcgtc caactggcta
     4561 agtaagttgc gagggtgcgt aatactacat cgccgattaa atgcccgtag gtatcattaa
     4621 ccaatttaaa tcggtctata tccaacaaca ttaaataaag attcagaggt tcagcattgc
     4681 gtaactgatg atcgaaggat tcatcaagaa cccgacgacc cggcaatccc gtcagaacat
     4741 ccatactgct acggatcgtc agcaaataaa ttttgtaatc ggttaatgcc gcagtaaaag
     4801 aaagcaaccc ttcctgaaaa gcgtcgaaat gcgcgtcctg ccagtgattt tcaacaatag
     4861 ccagcattaa ttcccgacca cagttatgca tatgttgatg ggcagaatcc attagccgaa
     4921 cgtaaggtaa ttcatcgtta tcgagtggcc ccagatgatc aatccaccga ccaaactggc
     4981 acagtccata agaatggtta tccgttattt ctggctcact ggcatctctc gcgaccacgc
     5041 tgtgaaacat acttaccagc cactggtagt gggcatcgat agccttattg agatttaaca
     5101 agatggcatc aatttccgtt gtcttcttga tcattgccac tcctttttca cagttccttg
     5161 tgcgcgctat tctaacgaga gaaaagcaaa attacgtcaa tattttcata gaaatccgaa
     5221 gttatgagca tctctgagat aacattgtga tttaaaacaa aatcagcaga taaaaaagtg
     5281 tttaattcta taaattaact ctgcattatc gcaattaaaa ggatgacaaa tagcataacc
     5341 caatacccta atggcccagt agttcaggcc atcaggctaa tttattttta tttctgcaaa
     5401 tgagtgaccc gaacgacggt cggcgcgctt ttcttatcca gactgccgct aatgttgctc
     5461 atctggtccg gctgaatttg aaaattatta taacggaagg gtatacaaaa aaagtaattt
     5521 tgctggcgac tgcgaaacgg cattcgcatt agcggtgaag tttgccctcg cccagctgct
     5581 gcaattactg ctataacgag gctggtctgg cgataaatcc aggccagcca ttggttgtgt
     5641 ttatacgttc aagccacgat gttgcagcat cggcataatc tgaggtgcct taccgcgcca
     5701 ttgtcgatac aggcgttcca gatcttcgct gttacctctg gaaagaatcg cctcgcgaaa
     5761 acgccgccca ttttcacgcg ttaatccgcc ctgctcaaca aaccactggt aaccatcatc
     5821 ggccaacatt tgcgtccaca gataagcgta ataacctgcg gcatatccgc caccaaaaat
     5881 atgagcgaaa taactgctgc gatagcgtgg cggtatagca ggaagatcca tattttccgc
     5941 caccagcgcc cgcaattcga aatcatcgac atcctgcatt gcttcgtttt cttccaggca
     6001 atgccagcgc atatcgagaa gtgcggcgct aagcagttcg ctcatctcat accctttatt
     6061 gaacaggctg gcattacgca ttttctgttg aagttcgtca ggcattgcgg ctctgctctg
     6121 ataatgccgg gcgtagcgag cgaatacctg cggatttgtt gcccagtgtt cgttgatttg
     6181 cgacggaaat tcgacaaaat cacgcggcgt gttggtgccg gaaagcgtgg cataacgctg
     6241 gcgggcaaaa aggccgtgca gcgtatgacc aaattcatgg aataaggtta ttacatcatc
     6301 ccagagtaac aacgcaggct caccggcagc gggtttctga taattgcaga cgttataaat
     6361 taccgggtgc gtttcattca gcgttgattg ctcaacaaaa ttgcccatcc atgcgccgcc
     6421 gctttttgaa tcacgggcga agaaatcacc gtaaaataac gccagcccca cgccattatg
     6481 atcaaaaatt tcccacacac gaacgtcagg atggtagaca ggaatatcaa aacgttcgac
     6541 aaacttaata ccgaagagct gattcgcagt ccagaatacg ccttcattca acaccgtgtt
     6601 taattcgaaa taaggcttga gctgcgcttc atcgagatcg aatttctcac gtcttacctg
     6661 ctcggcataa aatgcccagt cccacggctg cgcgctaaac ccgccctgct gcttatcgat
     6721 aaccgcctgt atggaggcta attcatcgct cgcacgttga cgcgccgcag gaacaatttc
     6781 ccgcataaag ttgagcgctg cttctggcgt ttttgccatc tgatcggcga ttttccatgc
     6841 ggcataatga ggaaaaccaa gcagtttcgc ctgctgcgcg cggatctcca ccagacgttg
     6901 aatgatagcg cgggtatcat tggcatcatt tttttccgct cgcgtccagc ccgcagtaaa
     6961 cagtttttca cgcgtcgcgc gatcgcgcag ttcagcaagc gctggttgct gggtggtatt
     7021 cagcagcgga atcagccagt tgttatccag acctttctcg cgagccgcct ctgccgccag
     7081 cgcaatctct tgttcactca ttcctgccag ctgcgcgata tcgttcacaa ccagaccgcc
     7141 ggatttattt gctgccagta accgctggtt aaactggctg gtcagggtcg cagcttctgt
     7201 attcagtact tttaattttg ctttatcagc ttgctcaagt ttggctccgg caaggacaaa
     7261 acgttgatga atcacctcca ccaggcggat ggattcacta tcaagcccca gggattcacg
     7321 gcgttgccag acagcatcta cccgcgcgaa taattccccg ttcagataga tatcattcgc
     7381 aagttccgcc agttcagcgg aaaactgttc atcaagacgc tgtaattcat cattggtatg
     7441 cgccgcagtc atcgcaaaaa agacgctggt aacgcgggta agtaattctc cgctttgttc
     7501 cagtgccaga atagtattgt tgaaatcagg tgtttgcggg ttaagcgcga tggcagctat
     7561 ttctgcccgc ttttgctgta ttccctcatc gaatgccggg cgatagtgat ggttggcaat
     7621 ttgatcaaaa tggggagcca gatacggcag tgtgctttgc acaaggaaag gattcattgt
     7681 tgtcattttc ttctcctgaa cgcgaggtgt tccatagcgt aggcttactg ataacggagt
     7741 gcaatcttgc aaaccagtat tacccgctct taagcatcac gtgctatgtt attgacacac
     7801 aaaagcgttg aggaacagtg agatgatcgt tttagtaact ggagcaacgg caggttttgg
     7861 tgaatgcatt actcgtcgtt ttattcaaca agggcataaa gttatcgcca ctggccgtcg
     7921 ccaggagcgg ttacaggagt taaaagacga actgggagat aatctgtata tcgcccaact
     7981 ggacgttcgc aaccgcgccg ctattgaaga gatgctggca tcgctccctg ccgagtggag
     8041 caatattgat atcctggtaa acaatgccgg cctggcgttg ggcatggagc ctgcgcataa
     8101 agccagcatt gaagactggg aaacgatgat tgataccaac aacaaaggcc tggtatatat
     8161 gacgcgcgcc gtcttaccgg gtatggttga acgtaatcat ggtcatatta ttaacattgg
     8221 ctcaacggct ggtagctggc cgtatgccgg tggtaacgtt tacggtgcga cgaaagcgtt
     8281 tgttcgtcaa tttagcctga atctgcgtac ggacctgcat ggtacggcgg tgcgcgtcac
     8341 cgacatcgaa ccgggtctgg tgggtggcac cgagttttcc aatgtccgct ttaaaggcga
     8401 tgatggtaaa gcggaaaaaa cctatcaaaa taccgttgca ttgacaccag aagatgtcag
     8461 cgaagccgtc tggtgggtgt caacgctgcc tgctcacgtc aatatcaata ccctggaaat
     8521 gatgccggtt acccaaagct atgccggact gaatgtccac cgtcagtaat ttttataccc
     8581 ggcgtaactg ccgggttatt gcttgtcaca aaaaagtggt agactcatgc agttaactca
     8641 ctcacaagca agaacgaatg accgtcgaaa cgcaacttaa tcccacacag cctgtcaatc
     8701 agcagattta tcgtattctt cgccgcgaca ttgtccattg cctgattgca ccaggcacac
     8761 cgttgtcgga aaaagaagtt tctgttcgtt tcaatgtgtc acgccagccg gttcgtgaag
     8821 cctttattaa actggcggaa aacggtctga ttcaaattcg tccgcagcgt ggcagctacg
     8881 tcaacaaaat ttccatggcc caggtgcgca acggcagttt tatccgtcag gccattgagt
     8941 gcgcggtggc gcgtcgggcg gcgagcatga ttaccgaaag ccagtgctac caactggaac
     9001 aaaatcttca ccagcaacgc attgccattg agcgcaagca actggatgat ttttttgaac
     9061 ttgatgacaa cttccatcaa ctcctgacgc agattgccga ctgtcaactg gcgtgggata
     9121 ccattgagaa cctgaaagcg accgttgatc gcgtgcgcta tatgagtttc gaccacgttt
     9181 ctccaccaga aatgctgtta cgccagcatc ttgatatttt ctctgccctg caaaaacgtg
     9241 atggcgatgc ggtagaacgt gcaatgacgc aacatttgca ggaaatcagc gaatctgtgc
     9301 gccagatccg ccaggaaaac agcgactggt ttagcgaaga gtaattcatt tcctctcatc
     9361 ccatccgggg tgagagtctt ttcccccgac ttatggctca tgcatgcatc aaaaaagatg
     9421 tgagcttgat caaaaacaaa aaatatttca ctcgacagga gtatttatat tgcgcccgtt
     9481 acgtgggctt cgactgtaaa tcagaaagga gaaaacacct atgacgacct acgatcgtaa
     9541 ccgtaacgca atcaccactg gcagccgtgt tatggttagc ggcaccggtc acactggcaa
     9601 gatcctgtcg attgatactg aaggtctgac cgctgagcaa atccgccgcg gaaaaaccgt
     9661 agttgttgaa ggttgtgaag agaaactggc accactggac ctgattcgtc tcggcatgaa
     9721 ctaagcgtgt gaatgccgcc gatggcggca ttgctttttt tacttcacgg aatatttcgc
     9781 cacggtcgct ttcgcgccat gcgctaataa agacaagtac gcttccgtta cttttgtcgt
     9841 aaacaaacta ttgcctggta aatcatcacc aaagatcgcc ttaattgcca gcagtgactg
     9901 gacgcgcgct gtcccttcag cactactttg tactgccttc tgaataacag gtaacagtgg
     9961 atcactgatt tctatcggat ttccctgttc atcaacacca ccgacataac gcatccaacc
    10021 cgcgacgccc agcgccagca gatcgaactt gctgtcatgc gccagatgcc agcgaacaga
    10081 atccaacatc cgctgtggca atttctggct gccatccatc gcaatctgcc aggttcgatg
    10141 acgtaacgcc gggttgctat agcgttcaat taatcggtta gcgtaatctt gcaaatcaac
    10201 gccctgcact ttcagcgtcg gcacttgttc ctgcaacatt aaggcatacg ctgcatgacg
    10261 ataatgttca tcttccatgc agtcattaat gtgctgatat ccggcaagat accccagata
    10321 cgccaggaat gaatgactgc cattaagcat gcgcaacttc atctcttcat aaggcagcac
    10381 atcactaacc agttcggctc ccgctttttc ccattccgga cgtccggcaa caaagttatc
    10441 ttctattacc cactggcgga aaggttcaca ggcaacgcct gccggatcgc gcacaccggt
    10501 aagttgttcg attttcgcca gcgtatcctc tgtcactgcg ggcacaatac ggtccaccat
    10561 tgttgatggg aaagtcacgt tatcttcgat ccattgtgcc agttttacat caatggcttg
    10621 cgcataggaa gtgacaacgt cacacataac atgaccgttt tctggcatgt tgtcacatga
    10681 catgacggta aatgcgggaa gtcctgccgc tttacggcga gccagcgcct caacaatcac
    10741 ccctgttgca gttttcggct ggtggggatt ttgcacgtcg gcaacgacca tcgggtgatc
    10801 gagcattaac tgtccggtcg ccggagagtg gaaataccct ttttcggtga ttgtcagaga
    10861 gacaatcgcg atttgcggtt cacacatcgc agccaacacg gtttctaagc catctatttg
    10921 tacgtgcaag gcttttttaa cgacgccaac gacgcgagcc gtccacgcat cagccgacat
    10981 ttccgcaacg gtataaagat tatcttgctg ttgtaaatcg gcaatttgct gttcgccgcc
    11041 gattaagttg acctcataat atccccagtc actgaaatgt tccgtagcaa gaatatcggc
    11101 atacacgccc tgatgtgcac ggtgaaatgc accaaagcct aaatgaacaa ttcttggggc
    11161 caggttatta cgatcataaa cagggagcgt cgcttttgct gataacaaat gatttcccat
    11221 aacaattcct taaaattaaa tatagcaagc tacatgtttt gttatatgaa taaaaatccc
    11281 ctctccggta agagaaggga ttgaggattt acagacttct ggacggttgc gcagctctta
    11341 caacatgtgg ttgatcttct gcggcgtctt ccagcgcact taaatcacgg tctttcactt
    11401 ctggcatttt cagcgcagag attaaaccaa tcacagaata tgccatgatc ataatggcga
    11461 tcggatacca ggattccgtc atggtgcaga aaatccccgc caggatagga ccaaaaccgg
    11521 aagcgataag accaccaatt tctttagaaa tagccattcg ggtaaagcgg tttttacagc
    11581 cgaacatttc tgccatggta atgttttcca gagcaaataa tccaagcacc gcacagttat
    11641 gaatcacaat cagtgcaacc ataatggtgc tcggggcata gcttttatct acgatgatag
    11701 aaagcattgg ccatgccagc acaatcgcag aggtattcat aataatatac gggatccggc
    11761 gaccaatttt atcggata
//
LOCUS       DAZFFE010000122        11616 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFFE010000122 DAZFFE010000000
VERSION     DAZFFE010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.122"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..854
                     /locus_tag="ACHJ69_004010"
                     /pseudo
     CDS             1..854
                     /locus_tag="ACHJ69_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1197..2495
                     /gene="kgtP"
                     /locus_tag="ACHJ69_004011"
     CDS             1197..2495
                     /gene="kgtP"
                     /locus_tag="ACHJ69_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO5016891.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLIEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAVLREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAASLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLATIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLTLHRKGKGMRL"
     gene            complement(2492..2815)
                     /gene="yfiM"
                     /locus_tag="ACHJ69_004012"
     CDS             complement(2492..2815)
                     /gene="yfiM"
                     /locus_tag="ACHJ69_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO5016892.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDFAWDIAGASTGYTVW
                     QLTRH"
     gene            complement(2861..4216)
                     /gene="pssA"
                     /locus_tag="ACHJ69_004013"
     CDS             complement(2861..4216)
                     /gene="pssA"
                     /locus_tag="ACHJ69_004013"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO5016893.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(4330..6990)
                     /gene="pat"
                     /locus_tag="ACHJ69_004014"
     CDS             complement(4330..6990)
                     /gene="pat"
                     /locus_tag="ACHJ69_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO5016894.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHKDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWNAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     VISNPLDLRDDASCEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESALALIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKICARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLKLNLAQREES"
     gene            complement(7022..7720)
                     /gene="tapT"
                     /locus_tag="ACHJ69_004015"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(7022..7720)
                     /gene="tapT"
                     /locus_tag="ACHJ69_004015"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO5016895.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSLDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(7789..8208)
                     /gene="trxC"
                     /locus_tag="ACHJ69_004016"
     CDS             complement(7789..8208)
                     /gene="trxC"
                     /locus_tag="ACHJ69_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO5016896.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            8415..9452
                     /gene="yfiF"
                     /locus_tag="ACHJ69_004017"
     CDS             8415..9452
                     /gene="yfiF"
                     /locus_tag="ACHJ69_004017"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO5016897.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEESPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(9500..10189)
                     /gene="ung"
                     /locus_tag="ACHJ69_004018"
     CDS             complement(9500..10189)
                     /gene="ung"
                     /locus_tag="ACHJ69_004018"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO5016898.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGEKPI
                     DWMPVLPAESE"
     gene            10494..10877
                     /gene="grcA"
                     /locus_tag="ACHJ69_004019"
     CDS             10494..10877
                     /gene="grcA"
                     /locus_tag="ACHJ69_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO5016899.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10932..11519)
                     /gene="eamB"
                     /locus_tag="ACHJ69_004020"
     CDS             complement(10932..11519)
                     /gene="eamB"
                     /locus_tag="ACHJ69_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO5016900.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGITALSTFVLPQTQALSWVIGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
BASE COUNT         2892 a         2904 c         3026 g         2794 t
ORIGIN      
        1 agggcacgat gagctggtga gttggctgaa atgtcacaag attgaccatg cgcatatctg
       61 catcgaagcg accggcacct atatggaacc tgtcgcagag tgcctttacg atgctggcta
      121 catagtgtca gtcattaatc ctgcactggg taaagctttc gctcagagtg aaggactgcg
      181 taacaagact gataccgtgg atgccgcatg ctggcagagt tctgtcgtca gaagcgccct
      241 gcagcctggg aagcgcatca cccgcttgaa cgcgcgttgc atgccctggt agtacgccac
      301 caggcgctga cagatatgca caggcaggaa ctgaatcgca ctgaaacggc gcgggaagtc
      361 cagaggccga gcattgatgc tcaccttctg tggcttgaag cagagctgaa gcgtcttgag
      421 aagcagataa aagacctgac agacgatgat ccggatatga aacaccgcag gaaactgctg
      481 gaaagcatcc cgggtatcgg agagaaaaca tctgcggtat tgctggctta catggtctga
      541 aggaccgttt tgcccatgcc agacagttcg ccgcttttgc gggtctgaca ccacggcgtt
      601 atgaatcagg cagtagtgtg agaggggcaa gccggttgag taaagccgga catgtgtcgc
      661 ttcgcaggcc gttgtatatg cctgcaatgg tagccaccag taagactgag tggggacggg
      721 cgttggcagc taatggcaag aaaggaaagg tgattctcgg ctcgataatg cgtaagctgg
      781 cacaggtggc gtatggagtg ctgaagtcag gcgtgccgtt cgatgcgtca cggcataatc
      841 cggtagctgc gtaaaaatcg cggaagggat gaaacaacag cgcctgacgg cgctgtgtct
      901 ggcatgcctg caatccggga gactggacca ggaaaaaact tgctggccgt aacagtatct
      961 acgctcccac ataagccaga ttcaacagcg aatacgtctt ccccaaattg cccacttcca
     1021 tacttcctcc ttaccagaaa tctatcctta agctccttaa taaccatttt cctgctaact
     1081 aaattcatgg ttaaggttgc ataatgatac gcaacaaatg tataacattt ttcttaccaa
     1141 aaaataaaca aaagcgaccg acaaaagcat cggattacgg caggagacat aatggcatgg
     1201 ctgaaagtac tgtaacggca gacagcaaac tgacaagtag tgatactcgt cgccgtattt
     1261 gggcaattgt tggggcctct tcaggtaatc tgatcgagtg gttcgatttc tatgtctact
     1321 cgttctgttc actctacttc gcccacatct tcttcccttc cgggaacacg acgactcaac
     1381 tactgcaaac agcaggtgtt tttgctgcgg gatttctgat gcgcccaata ggcggttggc
     1441 tatttggccg catagccgat aaacatggtc gcaaaaaatc gatgctatta tcggtgtgta
     1501 tgatgtgttt cggctcactg gttattgcct gtttgccagg ctacgaaaca atcggtacct
     1561 gggctccggc attattgctt cttgctcgtt tattccaggg attatccgtt ggcggagaat
     1621 atggcaccag cgccacctat atgagtgaag ttgccgttga agggcgcaaa ggtttttacg
     1681 catcatttca gtatgtgacg ttgatcggcg ggcaactgct agctctactg gttgtcgtgg
     1741 ttttacaaca caccatggaa gacgctgtac tcagagagtg gggatggcgt attcctttcg
     1801 cgttaggggc tgtgttagct gttgtggcgt tgtggttacg tcgtcagtta gatgaaactt
     1861 cacaacaaga aacacgcgct ttaaaagaag ctggatctct gaaaggatta tggcgcaatc
     1921 gccgtgcatt catcatggtt ctcggtttta ccgctgcggc ctccctttgt ttctatacct
     1981 tcaccactta tatgcagaag tatctggtaa atactgcggg aatgcatgcc aacgtggcga
     2041 gtggcattat gactgccgca ttgtttgtat tcatgcttat tcaaccactt attggcgcgc
     2101 tgtcggataa gattggtcgc cgtacctcaa tgttatgttt cggttctctg gcaaccattt
     2161 ttaccgttcc tattctctca gcattgcaga acgtttcctc gccttatgcc gcttttggtc
     2221 tggtgatgtg cgccctgctg atagtgagtt tttatacttc aatcagtgga atactgaagg
     2281 ctgagatgtt cccggcacag gttcgcgcat taggcgttgg tctgtcatat gcggtcgcca
     2341 atgctatatt tggtggttcg gcggagtacg tagcgttgtc gctgaaatca ataggaatgg
     2401 aaacagcctt cttctggtat gtgaccttga tggccgtggt ggcgtttctg gtttcactaa
     2461 cattgcaccg taaaggaaaa gggatgcggc tttagtgacg ggtcagttgc cagacggtat
     2521 agccggtgct tgcaccggcg atatcccagg caaaatcctt ccagctccag ccgctcccct
     2581 cagggcggct atcccaaagc tcttttgacg cccccagact gacagagaac atcaatccaa
     2641 acatggcact gcgatcccgg ctcatcccct gatgctgtga atattcattt ccggcggcgg
     2701 aaagcatcgc cgaggcgata aagtgttgag ctttatcctg cccgctccag ctatcgttag
     2761 ccatatggct gcatccagaa agtaacaata gcgaacagac aaaaagaata cgcaaaacgt
     2821 ggcctccaaa aaacaaaccc cgtacaggac ggggttgtga ttacaggatg cggctaatta
     2881 atcggtcgat gcggatacgg cgcaaacggc ggatgagttt acgaaccttc accggataat
     2941 cggcaatact ttgcagatcg cgatagtgct taacgatggt ggtatgctcg cggatcagct
     3001 ccagttcttt ctctcgctgc ggcgcaagct ccagttgtgg atcgtggatc aaaatggcgt
     3061 tttccagatc cagacgccag gcgcgcgggt tcaggttatt accggtgatc aacatccact
     3121 tatcatcaac ccacatccct tttaggtgat aggtgttgtc gtcatctttc cataaccgaa
     3181 ccactagctg gtcagtattg acgtaatact gcaaacggct caggaaacga cgcagattga
     3241 tctcatagag ataaggcaat gcgccaatta tcttgaaagg ttcatcttcc ggaatgtaga
     3301 agtcattcgc ggttttatca ccaacaataa tttcgacctt tttcccttcg cgcagcaact
     3361 ggataatatt gcgcacaagg attgctggca ggttgaagta tggcgtacag atggttagtt
     3421 tctgctcggc acaaggcata agatggaaaa tggtcttgtt caacagactc gatttcccca
     3481 gccccactag cggcgttacg gaaagctgat cgttgtcggc atcgccctgg aaatgataag
     3541 cggcatcacg cagctcctgg cggaacagac gaatatcgtt cttgatttcc gggctttttg
     3601 gccgattaac atcatccaga cgattaacgc cgcggccatt cataatattc tgtgtaaccc
     3661 attcaaacat aatgtctgac atcttacggt tacggataag atgataacgg tcgtagcgat
     3721 atttatcgtg ctgatgcagg taaacatcgt tcaggctggc accgctataa agtacgctat
     3781 cgtcgatgat aaagccttta aagtgcagaa caccaagggc ttcacgggta ttgattggca
     3841 cgccataaac cggaacatct acgcccggat tttcctgcgc catgcggcag taccagtcag
     3901 cgttagtgtt agatgccgca gcgccaatgc gtccacgttg tgcacgatgc cagtcgacca
     3961 gcacccgcac atccagttcc ggacgctgcc ttttagcctc atacaacgcg ttcagaatgc
     4021 ctttgccacc gtcatcctgt tcgagataca gggcgacaat gcaaatgcgc tgcttcgcgc
     4081 tggctatttt ttccagcagc gtctcccgga agtcggcggg agcgtaaaag aaatcgacat
     4141 catcaactga ttgagaaatc ttgggtagtt gggcaaggtg ttgttgatgt ttattacgct
     4201 taaattttga caacatcaca gtgcatttct tctctgttca ttgaagggtc ctctgtgcaa
     4261 tgcagacgac ataagcgggc aataataaca ccagtcccga ttaagtggtc aacatttcca
     4321 gtaccttact catgattcct cgcgctgggc aagatttagc ttaagcccaa cgatcccctc
     4381 ttcgagctgg atatcaacgt taaacccgag cttgcgggcc agcgccacca tgccacgatt
     4441 gtttggcatc gtaataccat tcagacgttg cagtccgtga tctcgcgtat aggtaatcaa
     4501 tttttccatt aagcgtcgac ctaagcctaa ccctttgaga tccgagcgaa ccagcacggc
     4561 aaattcggca tcgatgttat cagggtcgga gatcgcacgc gtgacgccga ggatctcttc
     4621 cgtttgatca atacgtcgta ccgctacaaa tgccatttcc cgatcgtagt cgatctgcgt
     4681 catgttggct aaatcttcat gggtaaattc gttgatctcg ctaaagtagc ggtaataaag
     4741 atcttctttg gtgactcgcg aaatgaactg ctgaagttgt ggctcatctt ctggcaaaat
     4801 cgggcggaac aagcagcgtt caccgttttt caattctacc cactcttcca gctgatgtgg
     4861 ataagggcgc actgccagcc ggctctcgtt atcgccttca aacggcgcga tatccagcgt
     4921 gacatccagc gcggtaaatt cactgccaga agccagcaaa ggatgaatat ccagccgttg
     4981 aatttccggg caatcgacaa tcaagttgga aacctgcacc agaagctggc tcaagcctgc
     5041 aacatccaat ggacgcagtg cactgcgcgc acaaatcttt ttacttttta ttccctgaat
     5101 gaccagataa cgggccaggt tcatgttcag cggcggtaat gcgacgacgg cttgatcttc
     5161 aggacgccac tccacaccgc cttcacccag catgatcaac ggcccgaaaa ccggatcgtg
     5221 ctcaaccaca acccgcaact cctgagcgcc agcacggtta gccatacttt gcactaacag
     5281 gccgtggacc cgcgcctgcg gccaggccat ttttacgcga tcgaaaatag cgtttgccgc
     5341 ttgctggact tcattggctg tacgtaggta gagcatgacg ccctgaactt ccgatttatg
     5401 tggaatatcc ggcgagcgca atttcagtgc caccggatag ccaatctgtt cggcaatgtg
     5461 taccgcttcg gtgctatcac tggcaatcca ggtagggaga gtgttcatac catacgcttg
     5521 caggatgggc tgaacttcat gggtatcgag cgacgtagcc ccttcggcaa tcgcctgttg
     5581 caacagaaga tgcgcttctg cggtattgga agtcagattg ctgggcagtg ccggcgtttc
     5641 gcgtagttgc ttctggttac gtcggtactc caccatatgc ataaaagcgg tgatggttcc
     5701 ttccggggta cggtaggtcg gcagcccggc ttcgctgaat aaacgtcgcg cctcctgcga
     5761 agagtgttcg ccgcaccagt tcgtcagcag agagacatac ttgctgcgag ggtgatgctt
     5821 taccgcttca attaatgcca gtgcgctttc tgtggcggga gcagcagcac tgggcgaatg
     5881 aataaccatc agcgcatcaa aatcctggct gtggagtaga atgtccagcg ttttgacata
     5941 atgctcgcaa ctagcgtcat cgcgcagatc gagcgggtta gagattacta catgttctgg
     6001 cagcgcatcg cgtagtttct ggcaggtttc ttcacttagc gttgccagct tgccattgcg
     6061 tgaccataaa gcatccagcg ccagcgcagc tggcgctgca ccgttgctga taatcatcag
     6121 tcggtcgcca cgcaatgggc gcatatggct aagggtttct actgccgaaa acagctcgtg
     6181 ggtatcctgc actcgcaaca aaccggcgcg ctgaatagct gcattccagg caggatccat
     6241 tcctgccgtg gtgttcagta gtcgttgcgc cgccgggcta cgtccgcttt taatcaccag
     6301 aatcggttta ttacgtgagg cgctacgggc cgccgaaaca aaacgtcgcg catcacttaa
     6361 ttgttcgaga tagagcagga tggcgctggt tttactgtcg cgcgccaaat agtcgagcaa
     6421 ttcatcaacg tcgatatcca ggctgtcgcc gagcgcaata aagtaggaaa agcccatctc
     6481 acgctgttgc gcccagtcga ggatggtgtt ggagacagca gccgattgcg aaataaacgc
     6541 cagcttgccg cgtttaatcg gcacaggtga aaagctggca tttagacctt gccagggagc
     6601 cagtaagccc agactgtttg gtcctagcag gcgcatgtta tggcgcaggg cgcaggcgcg
     6661 gagatctttg tgttgcgatg ccggggcgga aagaataatg caggttttac agcctttctc
     6721 gccgagctct tccagaagag caagattacg gctggcattg gtacataaaa ccgcaaggtc
     6781 gggtgtaaag ggcaagctgg caatatccgg ccaggccaac acacccaaca ccgctttcca
     6841 ggctggcgtc accgggagta ccggtccgtt aaagcctccc gccagcaggt tacgcatcat
     6901 caggtaacct gcgcgattgg gtttcatcga tgcgccaatt accgcaatcg attttggtcg
     6961 caatagtgct tccagtcctc gctgactcat accggtctcc cgcagaagtg accgaatgat
     7021 tttaaacgct ttctaactgt tctgctgtga tgctacccag atgttgcgtt tttcctgcca
     7081 gatagcgtgt tttaaagcgg gtaaaatgct cgcctaaccc tgccgccgcc ccggtatcgc
     7141 cggccatatc taacagtgcg atggctacct cggcagtaca atattggcct tcagcctggg
     7201 cttcacgcag gcgataggca gaaagtcggg aaagatcgac ggaaatgacg ggaagattat
     7261 ccagatacgg acttttacga aacatcttgc gagcttcagg ccaggtacca tcgagcatga
     7321 taaatagcgg aggcttaccg gcaggtggtg tgaagatcac ttcccgttgc tcatcagcat
     7381 acgaggcggg aaagaccacc attggctgat agtccgggtt ttgtaccaga tccagcaaat
     7441 ctagcgaggg ttcggtacgc gaccattgaa acgcaacggt atcaggcaaa atatcagcaa
     7501 tcagacgccc ggtattactg ggcttcattg gctcggtgtc gaacatcagc aaacagaagc
     7561 gactttttgc ttgtgctggg gtaattgtcg aacagagaca taatttctct ggtaaaaggc
     7621 agcgttggca gcgacgaacg cgattaccgc gggcaagaaa aggacgtgtt gcgcgcgcaa
     7681 tacgctcggc gcgtaactgg agaacagcgt tttcggtcat aagagagcgt cgaaaaaacg
     7741 ccattgtcgc agaggagaaa acggggcaca agatgcgccc cggtaagatt aaagagattc
     7801 gttcagccag ctatcaaacg gcgctttcgg tactgcgcca ttaagcatgt cgacaacctg
     7861 accgtttttg aaaatcatga tcgtcggtat actacgaatt ccaaagcgac tgcttaattc
     7921 acgttcagct tcggtattca ctttcacaaa gcgcacttta ccgctacgct cttgcgcgac
     7981 atcttcaaaa attggtgcga aattacggca ggggccgcac cacggtgccc agaagtcgat
     8041 caccacaggt agatcatctt tcagcaattt gtcgagcgtt tcaccggtcg cattaatcac
     8101 ctctccgtca aacaagtcgt gaccgcagcg tccgcatttt gccgcatctt cgatccgatc
     8161 gtcgggaatg cgattgatgg cctgacaatg ggtacaaacg gtattcataa ctaacctcgg
     8221 gatgagtaag gagacttatc ggcaacgacg ccggtatgtt tctaatatgt tacatattat
     8281 cggggacatt gttccagaca acaatctgtt ttattcaata ggtatgatag tcgcaggctt
     8341 tggtacgaaa tcatggcgaa ccgcgcggac ttcgggtaat ctgcgcgctt cgcgcagcgc
     8401 tggtggagaa aagcatgaac gatgaaatga aaggtaaaag cggcaaggtc aaagtgatgt
     8461 atgtccgcag tgatgatgat tctgataaac gtacccacaa cccgcgtacc gggaaagggg
     8521 gcgggcgtcc aggaaaatct cgtgctgacg gtggccgtcg ccccgcccgc gatgacaaac
     8581 agagtcagcc ccgtgaccgc aagtgggaag agtcgccgtg gcgcacagtt tcccgcgcgc
     8641 cgggtgatga gacgccggaa aaggccgatc acggtggcat cagtggtaaa agttttattg
     8701 atccggaagt tttgcgtcgt cagcgtgcgg aagaaacccg cgtctacggc gaaaatgcct
     8761 gtcaggcact gttccagagc cgtccggaag cgattgttcg cgcctggttt atccagagtg
     8821 taacgccgcg ttttaaagaa gccttgcgct ggatggcagc aaaccgcaaa gcgtaccatg
     8881 tggtagatga agcggaattg acaaaagcgt caggcacgga acatcacggc ggcgtttgct
     8941 tcctgatcaa aaagcgtaac ggtacaaccg tgcagcagtg ggtaagccag gcaggcgcgc
     9001 aggattgtgt actggcgctg gaaaacgaat ctaacccgca taacctgggt ggcatgatgc
     9061 gcagctgcgc gcacttcggc gtgaaaggcg ttgtggtgca ggatgcggca ctgctggagt
     9121 cgggggcggc tatccgtacc gcagaaggcg gcgcagagca cgttcagccg attactggcg
     9181 acaacattgt taacgtgttg gatgatttcc gtcaggcagg ttacaccgta gtgaccactt
     9241 ccagcgagca ggggaaaccg ctgttcaaaa ccagtctgcc agcgaaaatg gtactggtgc
     9301 tgggtcagga atatgaaggg ttaccggatg ccgcacgcga tccgaacgat ttgcgcgtga
     9361 agattgatgg tactggcaac gttgccgggc taaatatctc tgttgcaacc ggcgttttgc
     9421 ttggtgaatg gtggcgtcag aataaagcct gataaatcag ccgggtggca acgctgccat
     9481 ccggcgtttt cctgcaaatt tactcacttt ctgccggtaa tactggcatc cagtcaatcg
     9541 gtttctcgcc acgttgttcc agccactgat ttgccagcac aaaatggttg cagccaaaga
     9601 acccacgatg cgccgaaagc ggtgatggat gcggcgcttt cagtacgtga tggcgttgtt
     9661 tatcaataat cgcccctttc ttctgtgcat gtgatcccca caacaaaaac accacgcctt
     9721 cgcggtgctg gttaatcagg ctgatgactt tatcggtgaa ggtctcccag ccgaggctgg
     9781 cgtgagaatg cgcctgacct gcgcgaaccg tcaacacagt attgagtagc agaacgccct
     9841 gacgcgccca gctttcaaga taaccatgat tagggcgggt gaaaccggga atagtatttt
     9901 ccagctcttt atacatattc agcagtgacg gcggtgtcgc aatgccgggg cgaacggaaa
     9961 atgccagacc atgcgcctgt cccggtccgt gataaggatc ctggccgaga atcaccactt
    10021 taacgtcacc caactctgta aagcggaacg cgttaaagac atccttttgt ggcggataga
    10081 tagtgacgcc ggactgccgc tcgctggcga cggtctgtag ggtattaaga aaatagggtt
    10141 gttgcttctc ttcagccagc acgtcatgcc aggttaattc gtttgccatc tcgctctccc
    10201 gcgaatcttc aatccgccta gcttaactgc ttctttctac agaacaaaat catgctcatt
    10261 ttttagactt aaacgagtgc aaaaaaatgt aaattggtaa aatttcacac ctgcataagt
    10321 tgatgtaaaa caataaaatc catttgcagc catacttgat gtgtggtttt tattgattta
    10381 aatcaaagat tcaagggtgt ttgaggagta tatatacact caagcaacaa tggttttacc
    10441 aattggccgc gacaggctga acaaatcaaa taattttgcc ggggaggcat cacatgatta
    10501 caggtatcca gattactaaa gccgctaacg acgatctgct gaactctttc tggctgctgg
    10561 acagcgaaaa aggcgaagcg cgttgcatcg ttgcgaaagc aggttttgca gaagacgaag
    10621 tggttgcagt aagcaaactg ggtgacattg agtaccgtga agttccagta gaagtgaaac
    10681 cagaagttcg cgttgaaggt ggtcaacacc tgaacgtaaa cgttctgcgt cgtgaaactc
    10741 tggaagatgc agttaagcat ccggaaaaat atccgcagct gaccatccgt gtatccggtt
    10801 atgcagttcg ctttaactct ctgactccgg aacagcagcg cgacgttatc gctcgtacct
    10861 ttactgaaag cctgtaaggc ttaagtagca agttactaag cggaagaggg cgacctcttc
    10921 cgcttttttc attaatagaa aatgcgtacc gcgcaataga ccagcagcag cgcgagcacg
    10981 atatttaact ggcgaccata ctggcgaaac aatcgctgaa acaggtgccc cgccagcgcc
    11041 cagcacacat tgccaaacgt cccaatcatc gccagcaaaa cgctgacgcc aattacccag
    11101 cttagcgcct gcgtttgcgg caggacaaac gtcgacagtg ctgtaatgcc atacaaaatg
    11161 attttgacgt tcacaaactg caaagcaaag ctggcccaaa aactgattgg ttttgtctga
    11221 agcccatctt cctttgttgg actggtagcg attttccacg ccagccagac aatatatgcc
    11281 gcccccgccc aactcaacag atgcactgct gctgggtcga taacagccag tgaaaacgaa
    11341 attcccgcgc atagcaacat gacgattaaa aatcccagac tcatccctgc cagcacgcga
    11401 gtactctgac gaaatccgtg cgttgtggct gagctaaggg cgagaatatt gttcggtcct
    11461 ggcgtcatgg cggtaatcag ggtgtaagtc caaaaagcac ttaaaagggt cggtgtcact
    11521 tgttatctcc tctgttttgg tagacagtac ccgacacgga atatttaaaa aaaatgaatg
    11581 tttttacatt ctaaattcga tattttcgat aggttt
//
LOCUS       DAZFFE010000123        11533 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFFE010000123 DAZFFE010000000
VERSION     DAZFFE010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.123"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(17..274)
                     /locus_tag="ACHJ69_004021"
                     /pseudo
     CDS             complement(17..274)
                     /locus_tag="ACHJ69_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            272..685
                     /locus_tag="ACHJ69_004022"
                     /pseudo
     CDS             272..685
                     /locus_tag="ACHJ69_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015377870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
     gene            1026..1406
                     /gene="crcB"
                     /locus_tag="ACHJ69_004023"
     CDS             1026..1406
                     /gene="crcB"
                     /locus_tag="ACHJ69_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO5016901.1"
                     /translation="MIKSLFAVIIGGSVGCTLRWLLSTRFNSLFPNLPPGTLVVNLLA
                     GLIIGTALAYFLRQPHLDPFWKLMITTGLCGGLSTFSTFSVEVFALLQAGNYIWALTS
                     VLVHVIGSLIMTALGFFIITILFA"
     gene            complement(1786..2979)
                     /locus_tag="ACHJ69_004024"
     CDS             complement(1786..2979)
                     /locus_tag="ACHJ69_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5016902.1"
                     /translation="MSSKIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISLTGSKTVVLWALGGYGVSFTLLGLGSVLM
                     ATGAVTTAVGLGILIIARIVYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLVIHPLAPVWMLMAAPVWAVVMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAMLLAAAMSMMQLGLSPALTRQFDTDTTTISQQVAWLLGLSAIAALIAQFVV
                     LRPQRLTPVALLLSAGVLMSSGLAIMLTEQLWLFYLGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLAASHTLGYGLCALLVPLASKTGVSIALIVTALFAAVLFTIVSACIWH
                     YRTIK"
     gene            3115..4839
                     /gene="iucA"
                     /locus_tag="ACHJ69_004025"
     CDS             3115..4839
                     /gene="iucA"
                     /locus_tag="ACHJ69_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFO5016903.1"
                     /translation="MILPSEKSATDVAAQCFLNALIRETKDWQLAEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPAHLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESYAHTQQTIDARHDWAILREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RQEAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQVWCQALFAKGLIRDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSVRTLSVKEVERGMRLARLAQTDGWQMLQARFPTFRVMQEDGWAGLRDL
                     NGNIMQESLFSLRENLLLEQPQSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATEWLDTIDEAQAENIFTREQLLRYFPYYLLVNSTFAVTAALGAAGLDSEANLM
                     ARVRTLLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4840..5787
                     /gene="iucB"
                     /locus_tag="ACHJ69_004026"
     CDS             4840..5787
                     /gene="iucB"
                     /locus_tag="ACHJ69_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFO5016904.1"
                     /translation="MSGANIVHSGYGLRCEKLDKPLNLGWGLDNSAVLHWPGELPTGW
                     LCDALDQIFIAAPQLSAVVLPWSEWCEEPQALTLFGQVQSDIIHRSAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQIAYLERQLTSKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGVTHYLLLNEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5787..7529
                     /gene="iucC"
                     /locus_tag="ACHJ69_004027"
     CDS             5787..7529
                     /gene="iucC"
                     /locus_tag="ACHJ69_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFO5016905.1"
                     /translation="MNHKDWDLVNRRLVAKMLSELEYEQVFHAESQGDDRYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCADEPVLAQTLLMQLKQVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARHGLSASDLINLNADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNEMDIHQLLTAAMDPQEFARFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            7526..8859
                     /gene="iucD"
                     /locus_tag="ACHJ69_004028"
                     /pseudo
     CDS             7526..8859
                     /gene="iucD"
                     /locus_tag="ACHJ69_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016528830.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
     gene            8885..11086
                     /gene="iutA"
                     /locus_tag="ACHJ69_004029"
     CDS             8885..11086
                     /gene="iutA"
                     /locus_tag="ACHJ69_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFO5016906.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETM
                     MEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAVFLQSGYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2796 a         2958 c         2950 g         2829 t
ORIGIN      
        1 cacgctaaga gtttaattaa ttgtttgtat tgaagtttaa tgatcttaac tcacccttaa
       61 gaatatcttc cggtaacgaa gacgtataat gccccagcat atttatatgg ctatgtatca
      121 gcagcgataa tcttgccgga tgctactcct caggagtttc tcccgatttg cgaagatgtg
      181 acaacgcctc ctgcatgtaa agtgtgtttc acagcacaac cgcattggtc accagcccca
      241 gagcacctag ttgatcttcc tgcccttaac ggtagctgaa gatgactggg ctggatggaa
      301 aatcctgcat ggtgcattgg gagagcagat agaattggtt ggcgacgatc tgtttgtgac
      361 caacgtaaag tatattcagc gcggcattga cgaaaacctt gccaattcag cattgattaa
      421 actcaaccag attggttccc tgagtgaaac ctttgatgcg gtacaacttt gccatgacaa
      481 caactggggc acctttatct cacaccgcag tggtgagaca gtggacagct ttatcgccga
      541 tatgacagtt gcaatgcgcg caggtcatct caaaacggga gctccatgcc ggggtgagcg
      601 tattgaaaaa tataaccagc ttatgagaat tgaggatgaa ctgggttcat cggcgcaatt
      661 tgccggaaag tctgccttta aatgacatta ttgtgccaat cccccaggct tacacgtcgg
      721 ggttattctg gaggaatgat gaaagctcac gtttttgtta tttctatgct tactggcatt
      781 gtcgtcacct atgccgtgtt acttcttggt tgcttgttca tcgataaaac gctccccact
      841 gtagatgttg tcattctgtc acttgtcgtg ggggcttctg ctcagcaatt gtcgagggta
      901 ttaatgtcaa taaatagaac cttcccttat cttgcatgta gtcaaaggat ataaattaac
      961 aatatcagtc aaatgagaac atattctgat attgttacga taaaaaatta ttttatgagg
     1021 ggtacatgat taaatcactt tttgccgtca ttatcggcgg ttctgttgga tgtactctgc
     1081 gctggttact gtccacacgc tttaacagtc tttttccaaa tctacctcca ggtacactgg
     1141 tggtgaacct ccttgccggg ttaattattg gtactgcgtt ggcgtacttt ttacgacaac
     1201 cacaccttga tcctttttgg aaattaatga taacaacggg actttgtgga ggtctgagta
     1261 ctttctctac attttctgtt gaagtattcg cattactcca ggcgggaaat tatatatggg
     1321 ctttgacgtc tgtacttgtt cacgtcattg gctccctcat catgacagct ttaggctttt
     1381 ttatcataac catacttttc gcatgagaat tcattaacgt tacaggttta tcaactaatg
     1441 tgacgcttag gatagaattt cgctccatgg tatgcgtttg cagtcattac agtctccatg
     1501 acgactgtaa tgactgcaaa cgcattacct gacatatcag tataaacaat gaattaacct
     1561 cttggcgtat tttttacatc actggccctc accccccaaa attctctctg aaaaattgtt
     1621 gcggttaacg ttgccaatgt atccacccat ttcagaagac gatctggcat cctggtggca
     1681 tgctgcgatg tgcttcaggt gagtgcactg ggcggtcagc acaggcggtt tcgacagcga
     1741 aattgcacag gacgttatgc cggctgtgag tacaacatca tagccttact tgattgtacg
     1801 gtaatgccaa atacatgcag acactatggt aaataataca gcggcaaata atgccgtcac
     1861 aatcagcgcg attgagacac ctgttttcga cgccagtggc accaacaatg cgcacagccc
     1921 atagccaagt gtgtgactgg ccgcaagcca gcccgcgcct gcgccgtcgg ccagcttatc
     1981 attcagtaaa agttgataag cgggtgttgc cagagcggcc ccaaatgaca gcactgcaca
     2041 gcccaggtaa aacaaccata gctgttcagt gagcatgata gccagaccac tactcatcaa
     2101 caccccggca ctcaggagca gagccactgg agtcaggcgc tgtggacgga gtaccacgaa
     2161 ctgcgcgata agcgcagcta ttgcggacag ccccaacaac cacgccacct gttggctaat
     2221 agtggtggta tcagtatcga actggcgagt aagggctggc gaaagtccaa gctgcatcat
     2281 gctcattgcc gcagccagta acattgcgca aagcagataa ggcaggcaat cccgcttcag
     2341 gctgacgctc ttgcgctccg gtgtgggctg tggtggtgtg ccgggcaacc gcagaagcat
     2401 caccacagcc cacaccggag ctgccatcag catccacact ggcgccagag ggtgaatgac
     2461 caacattgcc gccgcgcaca gcggcccgaa tagcctgccg cagctcaggc cggagctgat
     2521 ggttgccaga gcggccatgc gattcccttc tcccgctctc tgcaatgccc agacctgaca
     2581 ggctggcacc attgctgaca cggtcagacc gtagacgatc cgggcgatga ttaatattcc
     2641 caaccccacc gctgttgtta cggcaccggt agccatcagc acgcttccca gcccaagcag
     2701 ggtaaagctt acgccatatc ctcccagagc ccacagcacc acagtcttac tgccagtaag
     2761 ggaaatttgc tttccccacc atggggaaga tggcagaaac agcattgagc ccagcatcag
     2821 cagggcagcc catacagaca gactcagatt tgtctgtatg accagaacag ggagtacaac
     2881 cagcagaccg ttctgtccga taccgagaag accggcactg aacgccagtg gccagcagga
     2941 cagggttttt tggggcgtat cttcgatctt agatgacatt gtaatgtttc aacaccatgt
     3001 attaattgtg tttatttgta aaattaattt ttctgacaat aacatttctc attgataatg
     3061 agaatcatta ttgacataat tgttattatt ttactgtgta ggagctgttt gattatgatc
     3121 ctgccctctg aaaaatccgc cacagatgtg gctgcgcagt gcttcctgaa tgccttgatt
     3181 cgtgaaacca aagactggca actggcggaa tacccgccag acgaattgat tatcccgctg
     3241 gacgaacaga agtcgctcca tttcagagtg gcttatttct ccccaaccca gcatcaccgc
     3301 tttgcatttc ctgctcacct ggtcacggca tcaggcagtt atcctgtcga ctttaccact
     3361 ctctcccggc ttattattga taagctccgg catcaacttt ttctgccagt tcccctctgc
     3421 gaaactttcc accaacgcgt gctggaaagc tacgcccata cgcaacagac aattgatgcc
     3481 cgccatgact gggccatcct gcgtgaaaaa gcgttgaatt ttggcgaggc tgagcaggca
     3541 ctgctgacag gacacgcttt ccaccctgca cctaagtctc atgaaccgtt taaccgccag
     3601 gaagctgaac gatacctgcc tgacatggca cctcacttcc cgctgcggtg gttttcggtg
     3661 gataaaacgc aaatcgctgg tgaaagtctg catcttaacc ttcaacagcg gttgacgcga
     3721 tttgccgcag aaaatgcgcc ccagttactc aacgaattaa gtgacaatca atggctgttc
     3781 ccgctgcacc cgtggcaggg agaatatctt ttgcagcaag tgtggtgcca ggcacttttt
     3841 gctaaaggac ttatcagaga cttaggcgag gccggcacgt cgtggctgcc gaccacctct
     3901 tcccgctccc tctactgtgc taccagccgc gatatgatca agttctccct gagcgttcgg
     3961 ctgaccaact ccgtccgtac tctgtctgtg aaagaggtgg agcgaggaat gcgcctggca
     4021 cgtctggcgc aaaccgacgg ctggcagatg ctacaggccc gcttccctac tttccgggta
     4081 atgcaggagg acggctgggc cgggctgcgc gatcttaacg gcaacatcat gcaggaaagt
     4141 ttgttttccc tgcgtgaaaa cctgctgctg gagcagccgc aaagccagac caacgttctg
     4201 gtttccctga ctcaggccgc accggatggc ggtgattcgc tgctggtttc ggcggtaaaa
     4261 cgcctgagcg atcgcctcgg tatcactgtg caacaggccg cccacgcatg ggtcgatgct
     4321 tactgtcagc aggtgctgaa gcctctgttt acggctgaag cggattacgg cctggtgctg
     4381 ctggcgcatc agcaaaatat tcttgtgcag atgcttgggg atctgccggt cggatttatt
     4441 taccgtgact gccagggcag cgcgtttatg cctcacgcga cagagtggct cgataccatt
     4501 gacgaggcgc aggcggaaaa tattttcacc cgtgagcagt tgctgcgcta tttcccttat
     4561 tacctgctgg ttaactccac ttttgccgta actgctgcgc tgggtgctgc cgggctggac
     4621 agcgaagcga atctgatggc tcgtgtacga acattgctgg ctgaagtgcg tgaccaggtg
     4681 actcataaaa cctgtctcaa ctatgttctg gaaagtccgt actggaacgt aaaaggtaac
     4741 tttttctgtt atctgaacga tcataacgag aacaccatcg ttgacccttc ggtgatctat
     4801 tttgatttcg ctaacccgct gcaggctcag gaggtctgaa tgtctggggc aaacattgtt
     4861 cacagcggat atggactgcg ctgtgaaaaa ctcgacaagc ctctgaatct tggctggggg
     4921 ctggacaata gcgcggtgtt gcactggccc ggggagctgc caacagggtg gctgtgcgac
     4981 gcgctggatc agatatttat cgccgcacca caactttcag cagtggttct tccctggtcc
     5041 gaatggtgtg aggagccaca ggcgctgacg cttttcggac aggtacaaag cgacattatc
     5101 catcgttccg ctttctggca gttaccgtta tggctgagtt ctccggcaaa ccgggcctcc
     5161 ggtgaaatgg tttttgatgc agagcgtgag atttatttcc cgcagcgccc cccccgtccg
     5221 cagggtgaag tttatcgtcg ttacgatcca cgcattcgca ggatgctgag tttccgcatt
     5281 gccgatcccg tttctgatgc agaacgtttc actcgctgga tgaacgatcc gcgcgttgag
     5341 tatttctggg agcaaagtgg ctcactggag gtacagatcg cctatctgga acgccagctc
     5401 accagtaaac atgcattccc gctgattggt tgtttcgacg atcggccgtt cagttatttc
     5461 gaaatctact gggcggcgga agaccgcatt ggtcgccact attcgtggca gccctttgac
     5521 cgtggcctgc atctgctggt tggtgaacag caatggcgcg gagcccacta tgtgcaaagc
     5581 tggctgcgcg gggtgacaca ttacctgctg ctgaatgagc cacgtacgca gcgcacagta
     5641 ctggagccac gcaccgataa ccagcgcctg ttccgccatc ttgagcctgc gggataccgg
     5701 acaattaaag agttcgactt cccacataaa cgctcgcgca tggtgatggc ggatcgtcat
     5761 cacttcttca cggaggtcgg tctgtaatga atcacaaaga ctgggatttg gtcaaccgcc
     5821 gcctggtggc aaaaatgttg tctgagctgg agtatgagca ggttttccac gcggaatctc
     5881 agggcgatga ccgctactgc attaacctgc cgggagcaca atggcgcttc atcgctgaac
     5941 gtggtatctg gggctggctc tggattgatg ctcaaactct gcgctgcgcg gacgagccag
     6001 tactggctca gacgctgctg atgcagctaa agcaggtact gtcaatgagc gatgcaaccg
     6061 ttgctgagca tatgcaggat ttgtatgcca cgctgctggg cgacctgcaa ctactgaaag
     6121 cccgtcacgg gctgagcgcc agtgacctga ttaatcttaa tgccgaccgc ctgcaatgcc
     6181 tgctgagcgg tcatcctaaa ttcgttttta ataaaggtcg ccgtggctgg ggtaaagagg
     6241 cgctggaacg atatgcgcca gagtatgcca acaccttcag actgcactgg ctggcggtaa
     6301 aacgtgaaca tatgatctgg cgctgtgata acgagatgga tattcatcag ttgttgacgg
     6361 ccgcaatgga tccgcaggag tttgcccgct tcagtcaggt ctggcaggaa aacggactgg
     6421 atcataactg gctgccgctg ccggtacatc cgtggcagtg gcagcaaaaa atcgctaccg
     6481 acttcatcgc tgattttgcc gaaggcagga tggtgtctct cggcgagttt ggcgaccagt
     6541 ggctcgccca gcagtcgctg cgtaccctga ccaacgccag tcgccggggc gggctggata
     6601 tcaagctgcc gctgaccatc tacaacacct catgctaccg ggggatccct ggcagataca
     6661 tcgctgccgg accactggct tcacgctggc tacaacaggt ttttgcgacc gacgccaccc
     6721 tagtgcaaag cggcgcagtg atacttggtg aaccggctgc aggctatgtc tcccatgaag
     6781 gctatgccgc gcttgcccgg gctccctatc gctaccagga aatgcttggt gttatctggc
     6841 gggagaatcc gtgccgctgg ctgaaaccgg atgaaagtcc ggttctgatg gcaacactga
     6901 tggagtgcga cgaaaacaat cagccgctgg caggcgcata tatagaccgc tccgggctgg
     6961 acgctgaaac ctggcttacg caactgttcc gggtggtcgt ggttcctctg tatcacctgc
     7021 tttgccgcta cggtgtcgcg cttattgcac atggacaaaa tataactctc gccatgaaag
     7081 agggggttcc acagcgtgtt ctgctgaaag acttccaggg cgatatgcga ctggtgaaag
     7141 aagagttccc cgaaatggac tctttgcctc aggaggttcg tgatgttaca tcccgcctga
     7201 gtgcggacta cttaatccat gatttgcaga cgggtcactt cgtgacagta ctgcgtttta
     7261 tttcgccact gatggttcgt cttggcgtac ctgaaaggcg attttatcaa ctgctggcag
     7321 cagtgttgag tgattacatg aaaaaacatc cacaaatgtc agagcgtttt gcgcttttct
     7381 cacttttcag gccacaaatc attcgcgtgg tgctgaaccc ggtaaaactg acctggcctg
     7441 atctggatgg cggcagccgc atgctgccga attaccttga ggatctgcaa aatccgctgt
     7501 ggctggtaac tcaggaatat gaatcatgaa aaaaagtgtc gattttattg gtgtagggac
     7561 agggccattt aatctcagca ttgctgcgtt gtcacatcag atcgaagaac tggactgtct
     7621 cttctttgat gaacatcctc atttttcctg gcatccgggt atgctggtac cggattgtca
     7681 tatgcagacc gtctttctga aagatctggt cagtgctgtt gcacctacaa atccctacag
     7741 ttttgttaac tatctggtga agcacaaaaa gttctatcgc ttccttacaa gcagactacg
     7801 tacagtatcc cgtgaagagt tttctgacta cctccgctgg gctgctgaag atatgaataa
     7861 cctgtatttc agtcataccg ttgaaaacat tgatttcgat aaaaaacgtc gattgtttct
     7921 ggtgcaaacc agccagggag aatattttgc ccgcaatatc tgccttggta caggaaaaca
     7981 accttattta ccaccctgtg tgaagcatat gacacaatcc tgtttccatg ccagtgaaat
     8041 gaatcttcgt cggccggacc ttagtggaaa acggataacc gtggttggtg gaggacagag
     8101 tggtgcagac ctgttcctta atgcattacg cggggaatgg ggagaagcgg cggaaataaa
     8161 ctgggtctcc cggcgtaata attttaacgc actggatgag gctgcttttg ctgatgagta
     8221 ttttacacct gaatatattt caggcttctc cggactggag gaagatattc gccatcagtt
     8281 actggatgag cagaaaatga catcggatgg catcactgcc gattctttac tgaccattta
     8341 tcgtgagttg taccaccgtt ttgaagttct gagaaaacca agaaatatcc gtctgctacc
     8401 cagccgctcg gtaacaactc tggaaagtag tggtcctggc tggaagttgc tgatggagca
     8461 tcatctggat cagggcaggg agagcctgga aagtgatgtg gtgattttcg ccacaggtta
     8521 ccgttctgcg ttgccacaaa tacttccctc actgatgccc ctgatcacca tgcacgataa
     8581 gaacaccttt aaagtgcgtg atgacttcac tctggaatgg agtggcccga aagagaacaa
     8641 catcttcgtg gtcaacgcca gtatgcaaac ccatggcatc gccgaacccc agctcagcct
     8701 gatggcatgg agatctgcac gtattcttaa tcgcgtaatg ggacgtgatt tattcgatct
     8761 cagtatgccg cccgccctga ttcagtggcg cagcggcacc tagggaaaac gcagccggag
     8821 gctgcttctt taactcgcta cacagcatct ttgggctgat tttttccgcc cgtatggagg
     8881 aataatgatg ataagcaaaa agtatacgct ttgggctctc aacccactgc ttcttaccat
     8941 gatggcgcca gcagtcgctc aacaaaccga tgatgaaacg ttcgtggtgt ctgccaaccg
     9001 cagcaatcgc accgtagcgg agatggcgca aaccacctgg gttatcgaaa acgccgaact
     9061 ggaacagcag attcagggcg gcaaagagct taaagacgca ctggctcagc tgatccctgg
     9121 ccttgacgtc agcagccgga gccgcaccaa ctacggtatg aatgtgcgtg gccgcccgct
     9181 ggtcgtgctg gttgacggcg tgcgtctcaa ctcttcacgt accgacagcc gacaactgga
     9241 ctctatagat ccttttaata tcgaccatat tgaagtgatc tccggtgcga cgtccctgta
     9301 cggcggcggc agtaccggtg gcctgatcaa catcgtgacc aaaaaaggcc agccggaaac
     9361 catgatggag tttgaggctg gcaccaaaag tggctttagc agcagtaaag atcacgatga
     9421 acgcattgcc ggagctgtct ccggcggaaa tgagcatatc tccggacgtc tttccgtggc
     9481 atatcagaaa tttggcggct ggtttgacgg taacggcgat gccaccttgc ttgataacac
     9541 ccagaccggc ctgcagtact ccgatcggct ggacatcatg ggaactggta cgctgaacat
     9601 cgatgaatcc cggcagcttc agttgatcac acagtactat aaaagccagg gcgacgacga
     9661 ttacgggctt aatctcggga aaggcttctc tgccatcaga gggaccagca cgccattcgt
     9721 cagtaacggg ctgaattccg accgtattcc cggcactgag cggcatttga tcagcctgca
     9781 gtactctgac agcgcttttc tgggacagga gctggtcggt caggtttact accgcgatga
     9841 gtcgttgcga ttctacccgt tcccgacggt aaatgcgaac aaacaggtga cggctttctc
     9901 ttcgtcacag caggacaccg accagtacgg catgaaactg actctgaaca gcaaaccgat
     9961 ggacggctgg caaatcacct gggggctgga tgctgatcat gagcgcttta cctccaacca
    10021 gatgttcttc gacctggctc aggcaagcgc ttccggaggg ctgaacaaca agaagattta
    10081 caccaccggg cgctatccgt cgtatgacat caccaacctg gcggtcttcc tgcaatcagg
    10141 ctatgacatc aataatctct ttaccctcaa cggtggcgta cgctatcagt acactgaaaa
    10201 caagattgat gatttcatcg gctacgcgca gcaacggcag attgccgccg ggaaggctac
    10261 atccgccgac gccattcctg gcggctcagt cgattacgac aacttcctgt tcaacgccgg
    10321 tctgctgatg cacatcaccg aacgccagca ggcatggctc aacttctccc agggcgtgga
    10381 gctgccggac ccgggtaaat actatggtcg cggcatctat ggtgctgcag tgaacggcca
    10441 tcttcctcta acaaagagtg tgaacgtcag cgacagcaag ctggaaggcg tgaaagtcga
    10501 ttcttatgag ctgggctggc gctttactgg caataatctg cgtacccaaa tcgcggccta
    10561 ctattcgatt tctgataaga gcgtggtggc gaataaagat ctgaccatca gcgtggtgga
    10621 cgacaaacgc cgtatttacg gcgtggaagg tgcggtggac tacctgattc ctgatactga
    10681 ctggagtacc ggagtgaact tcaacgtgct gaaaactgag tctaaagtga acggtacctg
    10741 gcagaaatac gatgtgaaga cagcaagccc atcaaaagcg acagcctaca ttggctgggc
    10801 accggacccg tggagtctgc gcgtgcagag caccacctcc tttgacgtga gcgacgcgca
    10861 gggctacaag gtcgatggct ataccaccgt ggatctgctc ggcagttatc agcttccggt
    10921 gggtacactc agcttcagca ttgaaaacct cttcgaccgt gactacacca ctgtctgggg
    10981 gcagcgtgca ccactgtact acagcccggg ttacggccca gcgtcactgt acgactacaa
    11041 aggcaggggc cgaacctttg gtctgaacta ctctgtgctg ttctgaccgg tattccttta
    11101 caacaaaggt acgctgatat caacatggcc gctgacagcc aagttgatat catataatac
    11161 acgacataat ctgtagtcag ggaggataga ctctttactg actacagatt atgtcgtgtt
    11221 ccgtgctcat ttcctcaaaa aaatacaaga aaagaattag tattctaaca aaaagtgaaa
    11281 taaattgtat caaactccct cttttaatcc tgttgagtaa atcagctttt gcaataggat
    11341 tgaaagagtg taagtggaat ctcttccgga tactcgttac cgccgtggct agaatatcta
    11401 cggctgcggg ccagtaatag attaagtgat aacagctgtc tggaaatgta ggggcgtatc
    11461 cactaatcat aatcattgtg agataaatat tacggggcat tttataatga ataaaatata
    11521 ctctctaaaa tat
//
LOCUS       DAZFFE010000124        11282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFFE010000124 DAZFFE010000000
VERSION     DAZFFE010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.124"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            75..209
                     /locus_tag="ACHJ69_004030"
     CDS             75..209
                     /locus_tag="ACHJ69_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO5016907.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTEKGKGKWKIITA
                     "
     gene            188..292
                     /gene="speFL"
                     /locus_tag="ACHJ69_004031"
     CDS             188..292
                     /gene="speFL"
                     /locus_tag="ACHJ69_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFO5016908.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            602..2800
                     /gene="speF"
                     /locus_tag="ACHJ69_004032"
     CDS             602..2800
                     /gene="speF"
                     /locus_tag="ACHJ69_004032"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO5016909.1"
                     /translation="MSELKIAVSRSCPDCFSTHRECVNIDKSNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLELNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFKFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     EADAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2797..4116
                     /gene="potE"
                     /locus_tag="ACHJ69_004033"
     CDS             2797..4116
                     /gene="potE"
                     /locus_tag="ACHJ69_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO5016910.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYTLYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4173..5813)
                     /gene="pgm"
                     /locus_tag="ACHJ69_004034"
     CDS             complement(4173..5813)
                     /gene="pgm"
                     /locus_tag="ACHJ69_004034"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO5016911.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5839..6384)
                     /gene="seqA"
                     /locus_tag="ACHJ69_004035"
     CDS             complement(5839..6384)
                     /gene="seqA"
                     /locus_tag="ACHJ69_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO5016912.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6569..7333
                     /gene="ybfF"
                     /locus_tag="ACHJ69_004036"
     CDS             6569..7333
                     /gene="ybfF"
                     /locus_tag="ACHJ69_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5016913.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7473..7766
                     /gene="ybfE"
                     /locus_tag="ACHJ69_004037"
     CDS             7473..7766
                     /gene="ybfE"
                     /locus_tag="ACHJ69_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO5016914.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7906..8436
                     /gene="fldA"
                     /locus_tag="ACHJ69_004038"
     CDS             7906..8436
                     /gene="fldA"
                     /locus_tag="ACHJ69_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO5016915.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8646..8732
                     /gene="uof"
                     /locus_tag="ACHJ69_004039"
     CDS             8646..8732
                     /gene="uof"
                     /locus_tag="ACHJ69_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO5016916.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8725..9171
                     /gene="fur"
                     /locus_tag="ACHJ69_004040"
     CDS             8725..9171
                     /gene="fur"
                     /locus_tag="ACHJ69_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO5016917.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9255..9581)
                     /gene="chiQ"
                     /locus_tag="ACHJ69_004041"
     CDS             complement(9255..9581)
                     /gene="chiQ"
                     /locus_tag="ACHJ69_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO5016918.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9631..11037)
                     /gene="chiP"
                     /locus_tag="ACHJ69_004042"
     CDS             complement(9631..11037)
                     /gene="chiP"
                     /locus_tag="ACHJ69_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO5016919.1"
                     /translation="MRAFSGKRSTLALAIAGVTAMSGFMAIPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAVFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFILEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2783 a         2799 c         2771 g         2929 t
ORIGIN      
        1 aatgaaaata caacccacaa aattgcaaca tttaaacaac aaaacagcat ctaatatggc
       61 gctattcatc attaatgatt tattatttgg gatatgatcg ttttttgttg atcttcttca
      121 cagattatag ccatttcatg gatagaataa ccctaccttc aactgacaca gaaaaaggta
      181 aaggtaaatg gaaaataata accgcttaat gcctcatata aggcggacaa cccatatcat
      241 gaagtttgcc catcgtaata gttttgactt tcatttcttt aatgcccgct agtcttctga
      301 ctaaagggca ccccagcgta caggtctccc tgactttaag cattacaggt taatacctgt
      361 atccctcggt gctcatatac tgctaaccct tttaaactct aaataattcg agtcgcagca
      421 taggcaactt gaagtatgac gagtatagcc agttaccggg ctggtctggg ttattgcatc
      481 tgcaaaaagc aaactactga tttatttatc agcggtgggg ttttgctttt ttccggcgtg
      541 atcaatttct tctttgagaa attgaggacc tgctattacc tgaaataaag agatgaacaa
      601 aatgtcagaa ttaaaaattg ccgttagccg ttcttgcccg gattgttttt ccactcatcg
      661 tgaatgcgtg aatattgaca aaagtaatta tattgacgtt gccgccatta ttttatcagt
      721 caatgatgtt gaacgtggaa aactcgatga aatagacgct actggctatg gcattcctgt
      781 ttttattgca acggaaaatg aagaacgtgt accagcagaa tatctgccac gtatttctgg
      841 tgtcttcgag cattgcgaat cacggaaaga gttttatgga cgtcagttag aaaccgctgc
      901 cagccattat gaaacgcaac tgcgcccgcc cttcttccgc gcactggtcg attatgtcaa
      961 tcagggcaat agcgcttttg actgcccggg tcatcaggga ggtgagtttt tccgtcgcca
     1021 tccggcaggc aatcagttcg tggaatattt tggcgaaatg ctcttccgct ccgacttgtg
     1081 taatgccgac gttgcaatgg gcgatctact gattcatgaa ggtgcgccat gcattgcaca
     1141 gcaacatgcg gctaaagtgt ttaatgccga taaaacgtac tttgttttaa acggcacctc
     1201 ctcctccaac aaagtggttt taaacgccct gctgacaccg ggtgatctgg tgctgtttga
     1261 ccgcaataac cataagtcta accatcacgg ggccttgcta caggctggtg caacaccggt
     1321 ttatctggaa acagcacgta acccgtatgg ttttatcggt ggcattgatg cgcactgctt
     1381 cgaagaaagt tatctgcgtg aactgattac agaagttgca ccgcaacggg caaaagaagc
     1441 gcgtccgttc cgcctcgctg tgattcagtt aggcacctac gacggtacta tttataacgc
     1501 ccgccaggtg gtggataaaa tcggtcatct gtgtgactac atcctgtttg actcagcatg
     1561 ggtcggctat gaacagttta ttccgatgat ggcggactgt tcgccgctgc tgctggagct
     1621 taatgagaac gatccgggta ttctggttac gcaatctgtg cataaacaac aggctggttt
     1681 ttctcagact tcacaaattc ataaaaaaga cagccatatc aaagggcaac agcgttatgt
     1741 accgcacaaa cgcatgaaca acgcctttat gatgcacgcc tccaccagcc cgttctatcc
     1801 gctgtttgcc gcactggata tcaacgccaa aatgcatgaa ggtgtcagcg gtcgtaatat
     1861 gtggatggat tgtgtggtta acggtattaa tgcccgcaaa ttgatcctcg ataactgcca
     1921 gcacattcgc ccgttcgttc ctgaactggt ggatggtaaa ccctggcagt cgtatgaaac
     1981 agcgcaaatt gcggttgatc tgcgcttctt taaatttgta cccggtgagc actggcattc
     2041 ttttgaaggc tacgcagaga accaatactt tgtcgatccg tgcaaactgt tgctgacaac
     2101 tccaggtatt gatgcacgta acggcgaata tgaagcgttc ggtgtacccg caacgattct
     2161 tgctaacttc ctgcgcgaaa atggcgtagt gccggaaaaa tgcgatctta actccatcct
     2221 cttcctgctg actccggcag aagatatggc caaactgcag caacttgttg ccctgctggt
     2281 acgcttcgaa aaactactgg aagctgatgc gcctctggcg gaagtactgc cttccatcta
     2341 caaacagcat gaagagcgct acgccggtta tacccttcgt cagttgtgcc aggaaatgca
     2401 tgatttgtat gcccgccaca acgtgaaaca actgcaaaaa gagatgttcc gtaaggaaca
     2461 cttcccacgc gtcagcatga atccgcaaga agccaactac gcctacttac gcggtgaagt
     2521 ggagctggtt cgtctgccgg atgcagaagg ccgtatcgct gccgaaggtg cgcttcctta
     2581 tcctccgggt gtgctgtgtg ttgttcctgg tgaaatctgg ggtggtgctg ttctgcgtta
     2641 cttcagcgct ctggaagaag gcatcaacct gctgccaggt tttgcaccgg agctgcaggg
     2701 tgtctatatc gaagaacatg atggtcgtaa gcaagtttgg tgctatgtca tcaagcctcg
     2761 tgatgcgcaa agcaccctgt tgaaagggga aaaattatga gtcaggctaa atcgaacaaa
     2821 atgggtgtcg ttcagttaac catactgacg atggtcaaca tgatgggctc cggtatcatc
     2881 atgctgccga caaagcttgc cgaagtcggg acaatctcaa ttatctcctg gctggtgaca
     2941 gccgtaggct caatggcact ggcatgggca ttcgcaaaat gcggtatgtt cagccgtaaa
     3001 tcaggaggta tgggcggtta tgccgagtat gctttcggta aatccggtaa ctttatggcg
     3061 aactatacct acggcgtctc gctgctgatt gctaacgtcg cgattgctat ttcggcggtt
     3121 ggttacggca ccgaactgct cggcgcgagt ttgtcgccag tgcagattgg tcttgcgacc
     3181 atcggggtgc tgtggatttg taccgtggct aactttggtg gtgcgcgcat taccgggcaa
     3241 atcagtagca ttaccgtgtg gggggtcatt attccggtgg taggcctgtg cattatcggc
     3301 tggttctggt ttagcccgac actttacgtt gattcctgga atccgcatca tgcaccgttc
     3361 ttcagtgcgg taggttcttc catcgctatg acgctgtggg cttttcttgg tctggagtct
     3421 gcgtgtgcga atactgatgt agtggaaaac ccggaacgta atgtgccaat cgcggtactc
     3481 ggcggtacgt taggtgcggc ggtgatttat atcgtctcca ccaacgtgat tgccgggatt
     3541 gtgccaaata tggagctggc aaattcaacg gcaccatttg gtctggcctt cgcgcagatg
     3601 ttcacgccgg aagtgggtaa agtcattatg gcgctgatgg tgatgtcctg ctgcggttcg
     3661 ctgcttggct ggcagttcac cattgcccag gtgtttaaat cttcatctga tgaaggctac
     3721 ttccctaaaa ttttctcccg tgtaaccaaa gtggatgcac cggtgcaggg aatgttgacc
     3781 attgtgatta tccagagcgg gttggcgctg atgacgatta gcccgtcgct aaacagtcag
     3841 ttcaacgtgc tggttaacct ggccgtggtg accaatatca ttccgtatat tctgtcaatg
     3901 gcggcattag tcattattca gaaggtcgct aatgtgcccc cctcaaaagc gaaagttgca
     3961 aactttgttg cttttgttgg cgcgatgtac agcttttata cgctgtactc atccggggaa
     4021 gaagccatgc tatacggttc catcgtgacc ttcctcggtt ggacactgta tggtctggtc
     4081 tcaccacgct ttgaactgaa aaataaacac ggttaattaa gcaaagcaaa aaagggcgat
     4141 cttccgaccg ccctttttct tatttgatat gtttacgcgt ttttcagaac ttcgctaaca
     4201 atctcaaccg cttctttctc aatctgcttg cgatgttctt caccaagaaa gctttcgcag
     4261 tagatcttat atgcgtcttc cgtgcctgac ggacgcgcgg cgaaccagcc gttgtcagtc
     4321 atcactttca gaccgccgat agaagcgcca ttacccggtg ccgccgtcag acgcgcggtg
     4381 atcgggtcac ctgccagggt gctggcgctc accatttccg gagacagctt agacaacgcc
     4441 gctttttgtg cggaagtcgc tgctgcctgc aaacggttat agctcggcgc accaaagcgt
     4501 tttgccagtt cgttatagtg ttcctgcggg ttcttaccgg tgacagcggt gatttccgcc
     4561 gccagcagac acataatgat accgtcttta tcggtggacc acggcgtgcc gtcgaaacgc
     4621 aggaaggaag cgcctgcact ctcttcgccg ccaaagccga agctgccatc gaacaaacca
     4681 tcaacaaacc atttgaaacc taccggtact tctaccagct tacggcccaa atcattgacc
     4741 acacggtcga tcatcgcaga tgaaaccagc gttttaccga cagcaacatc tttgccccac
     4801 tgcggacgat gctggaacag gtagttgatc gccactgcca ggtagtggtt cggattcatc
     4861 aaacctgccg gagtgacgat accgtgacgg tcataatccg ggtcgttagc aaacgccaga
     4921 tcgaacttat cacgcagtgc cagcaggccc gccatcgcac actcggagga gcagtccata
     4981 cggatcgcac cgtctttatc gaggtgcata aagcggaagg tttgatcgac ctgatcgtta
     5041 acgatggtca ggttgaggtt gtaatactcg ccaatgcgct tccagtattc gataccggaa
     5101 ccacccagcg gatcaacgcc cagcgtcagg cccgctttct gaatcgccgc catatcaacg
     5161 atatcggcca acccttccac aaacggctgc accagatcct gctctttcac atgaccggat
     5221 gccatcgctt cgtcgaggga gatacgcttc acgcctttca ggccatcagc cagcagtgcg
     5281 ttggctctgt cttccaccac tttagtgacg ttggtatcag ccgggccacc atttggcgga
     5341 ttgtacttga taccaccatc ttccggcggg ttatgggacg gtgtaatcac gataccgtct
     5401 gccagcgggc cacctttttt attgtgaacc agaatggcat tggaaatggc aggtgtcgga
     5461 gtgaagccat tgttttcctg cacaatgaca tcaacgccgt tcgctgccag cacttccagt
     5521 acggaaatga acgcaggctc ggacagggcg tgagtatctt tacccacata gcaagggcca
     5581 gtgatgccgt ttttcgcacg ttcttcagca attgcctgag cgatcgccag aatgtgcggc
     5641 tcgttaaagc tgtggcgcgc tgcactgcca cggtgaccgg aagtaccgaa tttcaccgcg
     5701 tgctccgcat tccctgcttc tggtttcagt acataatatt gcgccgtcag ttgggcgacg
     5761 ttaatcaaat cactctgttg tgcaggttgg cctgcacgat tgtggattgc cattgctttg
     5821 tcctttgtct gcaacgtttt agatagttcc gcaaaccttc tcaatcaatt ccgccgggaa
     5881 ttgcatcgac tgcatgatgt gttcgatcat gctgcattta cggccggtgt tggtgttggt
     5941 gatcacccaa tacggcgtac caggcacatg tttcggcttg gtctgattac catttttcag
     6001 cagcgtttgt tcatctgccg caaagtaaac gcgtgtacga ccgtgcaacg attccgttgc
     6061 ttcggcaaac gcctgggcgt caagagaata tagtgtagac aacagcagca taaagcgatt
     6121 gaccgctcgc ttttgctctg cgtattcgtc cgaaagcaga agttcacgca ttgcgcgaac
     6181 cttgtcttta atcgttttga ccggcttcgc ttcgacgata gcaggtgacg caacgcgaac
     6241 ctctttcgtc accggagcag caggctgtga tgcggcggaa aatttcaaca tacgccgtaa
     6301 aatgtcggat gcgctctcgc cgatatgctt agtgtggctg gcaatatagc tgtagagttc
     6361 atcatcaact tcaatcgttt tcatcttaat ccagtgcagt gtcttagctg aatacaagtc
     6421 gccaggagta tatgaataaa tcccggcagc ggatagcgtc aagcctggct tgcagaaaaa
     6481 ctcggaataa accttatttg ttgcagccct gtggcacaat ggtcaacata ataccctaac
     6541 ccgacaacgc gaaaaaaaga actttgccat gaaattgaat atccgcgcgc aaactgcaca
     6601 aaaccagcac aataattctc ccatcgttct tgtccatggt ctgtttggca gcctcgacaa
     6661 ccttggcgta ctggctcgcg atctggtaaa cgatcacaat atcatccagg ttgatatgcg
     6721 taaccacggt ctttcaccga gagatccggt aatgaattac ccggcgatgg cgcaggatct
     6781 tgttgatacc ctggatgcac agcagatcga caaagcaaca tttatcggtc actccatggg
     6841 cggaaaagcg gtaatggctc ttactgcact agcccccgat cgcattgaca agctggtagc
     6901 gattgatatt gcgccggtcg attatcacgt tcgccgtcat gatgagattt tcgcggccat
     6961 caacgcagtc agtgaatcag acgcacaaac acgccagcaa gcagcggcaa taatgcgcca
     7021 gcatcttaat gaagaagggg tgattcagtt tctgttgaaa tcttttgttg acggggagtg
     7081 gcgctttaac gtgccagtat tgtgggatca gtatccgcat attgtgggtt gggagaaaat
     7141 cccggcatgg gatcatccta ccctgtttat ccctggcggc aattctccgt atgttagcga
     7201 gcagtaccgt gatgatttac tggcacaatt tccacaggct cgggcgcatg tgattgcagg
     7261 cgcgggtcac tgggtccatg ctgaaaaacc ggatgcggta ttacgcgcta tccgtcgcta
     7321 tctcaatgat taacgcaact gattaaaaac ccagcgtccg cgtcgctttc cgcgcgtgga
     7381 cgttggcgtg actcatcgcg ctgatgtatt atggcgcgct atccatccgg gcaggcgcct
     7441 ggctgattgt ttcccccgaa gtcaccaaga tcatggccaa agaacaaacg gaccgtacga
     7501 cattagatct gttcgcgcac gagcgtcgac cgggacgacc gaaaactaat ccgctttcgc
     7561 gcgatgaaca gctgcgtatt aataaacgca accaactaaa acgcgacaaa gtacgtggcc
     7621 ttaagcgtgt cgaactgaag ctgaacgcgg aagctgtcga ggcactgaac gagctggcgg
     7681 aatcgcgcaa tatgagccgt agcgagctga tcgaagagat gttaatgcag caactggcgg
     7741 cgctgcgtag tcagggcatc gtttaaattt ccactttcat gtagcacagt gtgcagtcct
     7801 gctcgtttgc tgattccgca agactgcctg ttctgctatg attgccttta tccgtgggca
     7861 attttccacc cccatttcaa taagtttcaa gaggttattt cactcatggc tatcactggc
     7921 atctttttcg gcagcgacac cggtaatacc gaaaatatcg caaaaatgat tcaaaaacag
     7981 cttggtaaag acgttgccga tgtccatgac attgcaaaaa gcagcaaaga agatctggaa
     8041 gcatatgaca ttctgctgct gggcatccca acctggtatt acggcgaagc gcagtgtgac
     8101 tgggatgact tcttcccgac tctcgaagag attgatttta atggtaaact ggttgcgctg
     8161 tttggttgtg gcgaccagga agattacgca gaatacttct gcgatgcatt gggcaccatc
     8221 cgcgacatca ttgaaccgcg cggcgcaact atcgttggtc actggccaac cgcgggctac
     8281 cacttcgaag cgtcaaaagg tctggctgat gatgaccact ttgtcggtct ggctatcgac
     8341 gaagatcgtc agccggaact gactgctgaa cgtgtagaaa aatgggttaa acaaatttct
     8401 gaagagctgc atctcgacga aattctcaat gcctgatgtg atgcggcgta gactcatgtc
     8461 tacgccgtat taatagataa tgccaatcaa aataattgct acaaatttgt aacttttgct
     8521 gttgtacctg tacaatgtcc cggtgttcaa gtggccttgc cgttgtaaat gtaagctgtg
     8581 ccacgttttt attaacaata tttgccaggg acttgtggtt ttcatttagg cgtggcaatt
     8641 ctataatgat acgcattatc tcaagagcaa attctgtcac ttcttctaat gaagtgaacc
     8701 gcttagtaac aggacagatt ccgcatgact gataacaata ccgccctaaa gaaagctggc
     8761 ctgaaagtaa cgcttcctcg cttgaaaatc ctggaagttc ttcaggagcc ggacaaccat
     8821 cacgtcagtg cggaagattt atacaaacgt ctgatcgata tgggtgaaga aattggtctg
     8881 gctacggtat atcgcgtact gaaccagttt gacgacgctg gtatcgtcac ccgccacaat
     8941 tttgaaggcg gtaaatccgt atttgaacta acacagcaac atcaccacga tcacctgatc
     9001 tgcctcgact gcggtaaggt tatcgaattt agtgatgatt ccatcgaagc tcgtcagcgt
     9061 gaaatcgccg caaaacatgg cattcgcctg actaaccaca gtctctatct ttacggtcac
     9121 tgtgccgaag gcgattgccg cgaagatgag cacgcacacg aaggcaaata agccagcctg
     9181 aacgagaaaa gccaacctgc aggttggctt ttttatacaa gggaaataag aaaagcgaga
     9241 gttacagctc tcacttattt gttattactg cgaatttcct gccagacctt atcacaatcg
     9301 gctttcaccg cctggtcatt accggtttgc gttgcgcgca gacactgctg ataatccagt
     9361 acgcgtacac tttcctggtc agcaaactgc tgatgttgtt tctctttctt cagcacgttt
     9421 aacacgctct ggcaggcttc aattttttcc ggcgaacctt gcgcggtgtt gatacaggcg
     9481 ctgtaagcct ctttcagacg actgtcttcc tgcggtgcgg ttgattgcgc acaagctacc
     9541 agccccgatg ccatcatggc gatgagaatc agttttttca tagcaaattc ctcaaacgtg
     9601 cggcaggcac aaaacctgcc gcgtcgggca tcagaagatg gtgaatggtg cgattaccat
     9661 gaatttcacg tcgcgctcat cctggaaaat gttgccgtaa ccaccgcccc agcttgggat
     9721 gtcggagtgg ttgtcatatt cggtgaaatg cagtttgaac atcgtgccct tggcacgacc
     9781 gtcctgaatg gtgtaaaccg catccaggct gtaggcagac tcttcaatag tccggttctt
     9841 gtcgtagtac gcatccggat tgctctgcca ggtcgcaggt ttagcatccc atgcgtaaac
     9901 gtaggaagcg ccgatggcga agcctggaag attccagttt ttcaggtcat acatcgcacc
     9961 gaagaagacc gctttttcgc cgttggcgtt gaagtcggaa cggttatccc accagatatc
    10021 caggcgaccg ttcgaggaag cgtaggttgg agtcatacgt tgcaggaagt atccttgctg
    10081 accgtcagcc ttaacccagg taccttcgag gcgcaaatcc actacgtcag ccgcccggta
    10141 accaaaggtc aacgcctgca gccaggcggt gccgtcataa agatcgttga cgctgcgatc
    10201 gtcaacttta tcgcgcgtac catagaactg gtagctggtg gttaacgggc taccggcgat
    10261 atcaaatttg tagctggctt tggcaaaata ttgatcgata tacccttcgg cctgaccaaa
    10321 cgccgcttcc agtatgaagt tatttttgaa gtcgtacttc gccccaaggg agtgtagata
    10381 atcaactttg gtggttttat cgttctgata aaactcatcc atttccagat gccacggtgc
    10441 tttgtattcg ttggtccaca tgtaggagaa actcaacgca ccagcatcgc cgtaatcaaa
    10501 attcgccccg gcctccgccc cctgataagt acccggcata aagctccagt gtggcgctaa
    10561 cagcgtctga ccggttggct gaatataacc tgccctcgcc caaaccggac cgtatttaaa
    10621 tttggccgct gctttataca ggcttatacc gcttttatcg ccggaccagt cttcgtcata
    10681 ggctttatta ctttttgaaa acgcgatttc gttcgggtga gagctgtcgc cgttttccgc
    10741 catttcaatc gccgtaaaca cggcaatatc aaggccgaac atatcagcag cataaccaga
    10801 ctgaaaatcg aggttggcgt tccaggtgga gtgagaaagg ttggttttgt atttatcgcc
    10861 gtcggtaaca tctttacggt cgcgttcacg ctgccagtaa tagataccgc cggttaaggt
    10921 tgaatcgtcg atgaatcctt cggcgcgagc ctccggaatt gccataaagc ccgacatagc
    10981 tgtaacaccg gcgatagcca gcgccagcgt actacgtttg ccactaaacg cacgcatggg
    11041 ttaatcctct ttgacgtata aattgctgca ccaaaggtga acagcgaaaa aaataaaaat
    11101 taaaaaagta gcgttgagaa aagtctcgct gactacagga acagactatt tttcgcgacg
    11161 cgaattatca atctctttct gtaaccagaa tgtaagaaca tgagcaaccg cacatatttt
    11221 gatgattttt gttacaaggc ttaagtttgc agttgaaagg cttgcagaag aataaaaacg
    11281 tg
//
LOCUS       DAZFFE010000125        11062 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZFFE010000125 DAZFFE010000000
VERSION     DAZFFE010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.125"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            92..853
                     /gene="adiY"
                     /locus_tag="ACHJ69_004043"
     CDS             92..853
                     /gene="adiY"
                     /locus_tag="ACHJ69_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO5016920.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            990..2327
                     /gene="adiC"
                     /locus_tag="ACHJ69_004044"
     CDS             990..2327
                     /gene="adiC"
                     /locus_tag="ACHJ69_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO5016921.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2431..4074
                     /gene="eptA"
                     /locus_tag="ACHJ69_004045"
     CDS             2431..4074
                     /gene="eptA"
                     /locus_tag="ACHJ69_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO5016922.1"
                     /translation="MLKRLLKRPSLNLFSWLLLATFYISVCLNIAFFKQVLQVLPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLGSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMITNIIDTTPAESYALMTPRMLLTLGLSGVLAAIIACWIKITPTTSRLRSVLFRGAN
                     ILISVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRSANLPLIR
                     IGEDAHRNPLMQNGKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGVCD
                     RVPHQNITALNLPGQCINGECYDEVLFHGLEDYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAANDILQTCRRVSE"
     gene            4071..4739
                     /gene="basR"
                     /locus_tag="ACHJ69_004046"
     CDS             4071..4739
                     /gene="basR"
                     /locus_tag="ACHJ69_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO5016923.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVSTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELNVGNLTLNMGRRQVWMSGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4740..5840
                     /gene="pmrB"
                     /locus_tag="ACHJ69_004047"
     CDS             4740..5840
                     /gene="pmrB"
                     /locus_tag="ACHJ69_004047"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO5016924.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIDAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMENVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWIRLKKDQYVANQI"
     gene            complement(5816..5905)
                     /gene="pmrR"
                     /locus_tag="ACHJ69_004048"
     CDS             complement(5816..5905)
                     /gene="pmrR"
                     /locus_tag="ACHJ69_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO5016925.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(6017..7519)
                     /gene="proP"
                     /locus_tag="ACHJ69_004049"
     CDS             complement(6017..7519)
                     /gene="proP"
                     /locus_tag="ACHJ69_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO5016926.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7783..8661)
                     /locus_tag="ACHJ69_004050"
     CDS             complement(7783..8661)
                     /locus_tag="ACHJ69_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO5016927.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKQASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIVDLYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGRPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVSTGGEHADFLSWLGKWSTADKQPVCWSVTQRWRTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            complement(8658..10886)
                     /gene="crfC"
                     /locus_tag="ACHJ69_004051"
     CDS             complement(8658..10886)
                     /gene="crfC"
                     /locus_tag="ACHJ69_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO5016928.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISQLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDHLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGIAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLYAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LQQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSTNHFLRQQQDAVNAQLAALFQD
                     DSESLSEMRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQAATDEQITRFGVVREIFSRWLNQPD
                     WGRGNEKFPTETVDYSVLQRALSAEVDLYCQQMAKVMAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2805 a         2885 c         2672 g         2700 t
ORIGIN      
        1 tttatattca tcatacaatt atataaccat ttcccggtat cgcttcgctt tagcgagaac
       61 tggtctttta ttcgcactca ggagtacatg tatgaggatt tgcagcgacc aaccttgtat
      121 tgttttattg actgaaaaag atgtctggat aagggtgaat gggaaagaac ctattagcct
      181 taaagctaac cacatggcgt tattaaattg tgaaaataat attatcgacg tctcctctct
      241 taataacact ttggttgctc atattagtca cgacatcatc aaagattacc tccggtttct
      301 gaataaagat ctctcgcaaa taccagtatg gcaacgtagc gctacgccca tcctcaccct
      361 gccatgcctg acgccagacg tctttcgcgt tgccgcgcaa catagcatga tgcccgctga
      421 aactgagtca gaaaaggaac gaacacgtgc attattattc actgtgctat cccgttttct
      481 cgacagtaaa aaattccttt cactaatgat gtatatgtta cgtaattgtg taagtgacag
      541 cgtttatcaa attattgaaa gcgatatcca caaagactgg aatcttagta tggtagccag
      601 ttgtttatgt cttagcccaa gtctgttaaa gaaaaagctg aaaagcgaaa acaccagtta
      661 tagccaaata atcaccacct gccgcatgcg ttatgccgta aatgaattaa tgatggacgg
      721 taaaaatatc tctcaggtat cacagtcctg cggctacaac agtacgtcgt actttatttc
      781 tgtctttaaa gacttctacg gtatgacgcc gctgcattat gttagtcagc acagagaacg
      841 cactgtcgcc tgatttttaa ccttaacgaa gagctatatt aataacggca tcagcgataa
      901 cccagccgat aataattcaa ctatcgaatg caggcgtatg atatgacgta atttattgtc
      961 acgaagctcg ccttcgcagg agtttaatta tgtcttcgga tgctgatgct cacaaagtgg
     1021 gcttaatccc cgtcaccctg atggtgtcgg ggaatattat ggggtcaggt gtttttctgt
     1081 tacctgcaaa cctggcctcc actggcggga ttgccattta tggatggttg gtgacgatta
     1141 tcggtgcgct ggggctctcg atggtatacg ccaaaatgtc gttcctcgac ccaagtcctg
     1201 gtggttctta cgcttacgcc cgccgctgct ttggcccgtt tctcggttat caaaccaacg
     1261 tcctctactg gctggcctgc tggatcggca atatcgccat ggtggtcatt ggcgtaggat
     1321 atttaagtta cttcttcccg attctgaaag acccattggt attaaccatc acctgcgtcg
     1381 tggtgctgtg gatcttcgtc ctgctgaaca ttgtcggtcc gaaaatgatc acccgcgtac
     1441 aggccgttgc taccgtgctg gcgctgatcc ccatcgtcgg gattgccgta tttggctggt
     1501 tctggttccg tggtgaaacc tatatggcgg catggaacgt cagcggtctg ggcaccttcg
     1561 gtgcgattca aagtaccctt aacgttacgc tgtggtcgtt catcggtgtg gaaagtgcct
     1621 ccgttgccgc aggtgtggtg aaaaacccga aacgcaacgt ccctatcgcc accattggcg
     1681 gggtattgat tgctgccgtt tgctatgtac tttctaccac cgcgattatg gggatgatcc
     1741 ctaatgccgc actgcgcgtt tctgcttcgc cattcggtga tgccgcacgg atggcgttgg
     1801 gtgacaccgc cggggccatt gtttccttct gcgcagctgc gggttgctta ggttcactgg
     1861 gcggctggac gttgctggcg ggtcaaacgg cgaaagccgc tgccgatgac ggactgttcc
     1921 caccgatttt tgcccgtgta aataaagcgg gcacaccagt ggcggggctg attatcgtcg
     1981 gtattttgat gaccatcttc cagctcagta gcatttcgcc aaacgcgacc aaagaattcg
     2041 gtctggtttc ttccgtgtcg gtcatcttta cactggtgcc atatctttac acctgtgcag
     2101 cgttactgct gctcggccac ggtcactttg gtaaagcgcg cccggcatac ctggcagtta
     2161 ctaccattgc cttcctctac tgcatctggg ccgtagtagg gtccggcgcg aaagaggtta
     2221 tgtggtcatt tgtcactctg atggtaatca ccgccatgta tgcgctgaat tacaaccggc
     2281 tgcataaaaa cccgtatccc ttagatgcac caataagcaa agattaattc cccttaatca
     2341 agcaaacata aaagccaacc ttaagaactt aaggttggct taattttgct ttgcgagcat
     2401 atgcgcactt tgttcgatgg aaacaccgtg atgttgaagc gcctactaaa aagaccctct
     2461 ttaaatttat tctcctggtt attacttgct actttttata tctcagtctg cctgaatatc
     2521 gcctttttta aacaggtact acaggtgtta ccgctggatt cactgcataa cgtgctggtt
     2581 ttcttgtcga tgccggtcgt cgcattcagc gtgattaata ttgtcctgac actaggctct
     2641 ttcttatggc ttaatcgtcc actcgcctgc ctgtttattc tggtcggtgc ggctgcacaa
     2701 tatttcatca tgacctatgg catcgtcatc gaccgttcaa tgatcaccaa tatcattgat
     2761 actacgccgg cggaaagtta tgccctgatg acgccacgaa tgttattaac gctggggtta
     2821 agtggcgtgt tagccgcgat tattgcctgc tggatcaaaa tcacccccac cacctcacgc
     2881 ctgcgcagcg ttcttttccg cggggccaac atcctgattt ctgtactgct gattctgctg
     2941 gttgccgcac tgttctacaa agactatgcc tcgctgtttc gcaacaacaa agagctggtg
     3001 aaatccttaa gcccgtccaa cagcattgtt gctagctggt cgtggtactc tcaccagcgg
     3061 tcagccaacc tgccactgat acgcattggt gaagatgctc accgcaaccc attaatgcaa
     3121 aatgggaaac gtaaaaacct gaccattttg atcgtcggag aaacgtcgcg cgcagagaat
     3181 ttctcgctga atggttatcc acgcgaaacc aatcctcgtc tggcaaaaga taacgtggtc
     3241 tatttcccta ataccgcctc ttgcggcacg gcaacggcag tttcagtacc atgcatgttc
     3301 tcggatatgc cgcgtgagca ctacaaagaa gagctggcac agcaccagga aggcgtgctg
     3361 gatatcattc agcgagcggg catcaacgtg ctgtggaatg acaacgatgg cggctgtaaa
     3421 ggcgtttgcg atcgcgtacc tcaccagaac atcaccgcgc tgaacctgcc tggtcagtgc
     3481 atcaacggcg aatgctatga tgaagtgctg ttccacgggc tggaagatta catcaataac
     3541 ctgcagggtg atggcgtgat tgtcttacac accatcggca gccacggccc gacctattac
     3601 aaccgctatc cgccgcagtt caggaaattt accccaacct gcgacactaa cgagatccag
     3661 acctgtacca aagagcaact ggtgaacact tacgacaaca cgctggttta cgtcgactat
     3721 attgttgata aagcgattaa tctgctgaaa gaacatcagg ataaatttac caccagcctg
     3781 gtttatcttt ctgaccacgg tgaatcgtta ggtgaaaatg gcatctatct gcacggtctg
     3841 ccttatgcca tcgccccgga tagccaaaaa caggtgccga tgctgctgtg gctatcggag
     3901 gattatcaaa aacggtatca ggttgaccag aactgcctgc aaaaacaggc gcaaacgcaa
     3961 cactattcac aagacaattt attctcaacc ttattgggcc tgactggcgt tgagacgaag
     4021 tattaccagg ctgcgaatga tattctgcaa acttgcagga gagtgagtga atgaaaattc
     4081 tgattgttga agacgatacg ctgttattgc agggattgat tctggcggcg caaaccgaag
     4141 gctacgcgtg cgatggcgtg agcaccgcgc ggatggcgga acaaagcctt gaggccggtc
     4201 attacagcct ggtggtactg gatttaggct tacccgatga agatgggctg cattttcttg
     4261 cccgtatccg gcagaaaaaa tacactctgc cggtgttaat tctcaccgcc cgcgataccc
     4321 ttaccgacaa aatcgccggg ctggatgtcg gggccgacga ttatctggtg aagccttttg
     4381 cgctggaaga gttgcacgcc cgtatccgcg ccctgctacg acgccataat aatcagggcg
     4441 aaagtgagct gaacgttggc aacctgacgc tgaatatggg tcgccgccag gtatggatga
     4501 gcggagaaga actgattctg acgcccaaag aatatgctct gctgtcccgc ctgatgctta
     4561 aagcaggcag cccggtgcat cgggaaattc tctacaacga tatctacaac tgggacaacg
     4621 agccctcgac caacaccctg gaagtgcata tccataacct gcgcgacaaa gtgggcaaag
     4681 cccgtatccg caccgtgcgc ggatttggct atatgctggt cgcaaatgag gaaaactaat
     4741 tgaatctgat gcgttttctg cgccgaccaa tatcgctgcg ccaacggctg atattgacca
     4801 tcggggccat tttgttggta tttgagctga tcagcgtctt ctggctatgg catgaaagta
     4861 ccgagcagat tcagctgttt gagcaggcgc tacgcgacaa tcgcaacaac gatcgccaca
     4921 tcatgcgtga gatccgcgag gcggtcgcca gcctgattgt ccccggcgtc tttatggtta
     4981 gcctgacgct atttatctgc tatcaggcgg tacgccgcat cacccgaccg ctggcggagc
     5041 tgcaaaaaga gctggaagcg cgcaccgccg acaacttaac gcccattgcc attcatagcg
     5101 ccaccctcga aatcgatgcg gtggtttcgg cgttaaacga tctggtcagt cgcctgacca
     5161 gcacgctgga taacgaaagg ttgtttaccg ctgacgtcgc gcacgaactg cgaacgccac
     5221 tggcgggggt gcgtttgcat ctggaactgc tggcgaaaac gcatcacatt gatgtagcac
     5281 cgttagtggc acgacttgat cagatgatgg agaacgtctc ccagctgctg caactggcgc
     5341 gtgccggaca gtcattttct tccggtaatt atcaacatgt aaaactgctg gaagatgtca
     5401 ttctcccctc gtatgacgaa ctcagtacca tgctcgacca gcgccagcaa accctgctat
     5461 tgccagagag cgccgccgac atcaccgttc agggtgatgc aaccctgctg cggatgttat
     5521 tgcgaaacct ggtagaaaac gcccatcgtt acagcccaca aggcagcaac attatgatta
     5581 agctgcaaga agatggcgga gcggtcatgg ccgttgaaga tgaagggccg ggtattgatg
     5641 agagtaaatg cggggagttg agtaaagcgt ttgtacgaat ggacagccgt tatggcggaa
     5701 ttggtctggg gttaagtatt gtcagccgca ttacacagtt gcatcacggg cagtttttcc
     5761 tgcaaaaccg gcaagagact tccggcacgc gggcctggat acggctgaaa aaagatcagt
     5821 acgtggcaaa ccagatataa agaaagctgc tgaccaccag cacgctgaac acggtagtta
     5881 aactttcata aacacggttt ttcattgcac tctcctccgt taacctggag gagagtatgc
     5941 gcgtcagaga ttaagtgaac cttaagagtt caattgacct gataagacag cgtcacatca
     6001 ggccatccgt ttcagcttat tcatcaattc gcggatgttg ctgcaccagg cgggtacgtt
     6061 tcgcctgcaa atcggcaatc tcgtggtcaa tatcatcgat tttctgctcg atattatcgt
     6121 aatgctcgac gagaatttcc ttcgcttcct gtatatctga cgccgctggt gtcgcacctt
     6181 tcaacggtcg attcgccgtc tctttcatgg ttacgccggt gattaaacca accaccgcca
     6241 ctaccatcag gtaataggca ggcatcatca gattctgcga gctttcgacc agccaggccg
     6301 ccagcgttgg cgtcagaccg gcaaccagca ccgaaatatt aaatgccgcc gccagcgcgc
     6361 tgtaacggat atgcgtcggg aacatcgcag gcaaggtaga agccataacg cccgtaaagc
     6421 agttaaggat caccgccagc atcaataagc cggcaaaaat caggccgatg acgttactgt
     6481 taatcagaat aaacgccggg atcgccaaca caaacaatgc aacactacca agaagcacaa
     6541 acggacgacg gccaaaacgg tcactcagca agcccatcac cggttgaaca aacagcatac
     6601 cgatcataat ggcgataata atcagcaccc cgtggtcttc ggagtaatgc aggttatgcg
     6661 acaaataact cggcatatag gtcagcaaca tgtagtaagt cacgttggtg gcaattacca
     6721 gaccaataca tgtcaacagg ctgcgccagt gtttggtggc aatctcttta aacgagactt
     6781 tcgggccatc ctgcaaacct tcgcggtcgc cctgttccag tttatcgaca tgctgctgga
     6841 acgccggagt ctcttccagc gcatggcgca ggtaaagccc gataatccct aacggcagag
     6901 cgataaagaa cggaatacgc cagccccaat cgaggaagtt ctcttcgccg acaatggtcg
     6961 aaattaacac cactacgcca gcacccagca caaacccggc aatagaacca aagtccagcc
     7021 agctgcccat aaagccacgt ttacggtcag gggagtattc cgcaacaaat attgacgccc
     7081 cggtatattc accgccgacc gagaaacctt gtgccatctt acagatcaac agcagaatcg
     7141 gtgcccaaat accaatcgtg tcgtaggacg gtattaagcc aatacagaac gtactgatcg
     7201 acataatcac aatagtgata gcgaggatct tctggcgacc atatttatcg cccaacatac
     7261 caaagaagag cccgccaagc ggtcgaatca gaaagggaac ggagaaagtg gcgagtgcag
     7321 caaccatctg cacgctgggg tcagcccccg ggaaaaaaac tttacctaat gcgtaagcaa
     7381 caaaaccata aacaccaaaa tcgaaccatt ccattgcatt acccagtgat gctgcggtaa
     7441 tggctttacg cagtttacca tcatcaataa tggtgacatc acgaagggta atcggtttta
     7501 cttttttcct tttcagcata gctttcctcg cagagttggc ccatttccgc aaacgccaga
     7561 aaaatcagcg cgacggccgc aaaaggggag cgggaattgc aggatgcaat ctctgtaaca
     7621 agcgtagcgg gtttgcttac accctccggt taaggaatgt acaatcaaac cagttgacgc
     7681 ctgaattggg cagttaatga cctttttacc ctaccagtgt ttaaatttgt gatcaacttc
     7741 acacatgtat ttcgtttagg actcattggt gtaactgatt atttacacca aatttacaga
     7801 gaagatttcc tcgaccattg ctcccgccag acgctgcgct gaacagagtc gtggcatccc
     7861 cagtgcgaca gttcgccagc gttgggtcac tgaccagcaa acgggttgtt tatctgccgt
     7921 ggaccacttt cccagccaac ttaaaaaatc agcatgctcc ccgcccgtcg aaacggtcga
     7981 agaggcaaac cactgatggt aatagtgacg cgtggtcggg gccgcattga tactctctgc
     8041 caaatgttgt gtcgctttca cgcgcaaatc acccagcaac aacgccaccg cttgcggtct
     8101 tcccagacgg caggcatcgc caaatgcacc ccagcgcaac tcctccagaa cgataaagca
     8161 gcgcccaggc aacgaccagg cgttatacgc cccagcacgc cagcgggtaa aaatttgctc
     8221 gctctgttca cccgcctgca ccaataggcc tcgttgcgtt aacgcctttt ccttccagcg
     8281 acaacgggct gaaaaccact catacaagtc gacaatttgc tgggggagct ccgggaaagc
     8341 agagtgctgg cggtgatcca ttttttgcag cagcgccaga tgctgcgcga tctgcgtcag
     8401 cgccagatga ccgggatcca gcatcgacgc ctgtttttcc accaatgtca gacgcaggct
     8461 aagcggcgca tttaacatgc cgccgttcgt ccatgtctga agctgcacgc gcgcaagaag
     8521 ttcctgcatc agtcgttcac gaaactgctg ctgatgggct tgtggaaggc gggcatcatc
     8581 gccctgcgcc agatccacta aaaagcgcgg atgaaccgac tccagcacgc gaccggggcc
     8641 gtccagtaac gtcttggtca atatcgttct gccgtgaaaa gtgtttgaat atcatcgcgt
     8701 aacagctgag cgtcggtgta aatccagccg tgagtcatca cagtctgctg caattgctgt
     8761 tgcatcagcc tgaccacgga ttcattttgt tgacgcagag ccagactttc gcgtaaacgc
     8821 gtctgtaatt ccgtcaaaca tgaagcgaac tcagcgaaaa aagtattcat gcctgccgta
     8881 acagattcat cgacctgctc tgccataact ttagccattt gttggcaata aagatcgact
     8941 tctgcgctta atgctcgttg caacacactg taatcaaccg tctctgtcgg gaatttctca
     9001 tttccccgtc cccagtcggg ctgattcaac cagcgcgaaa aaatctcacg cacaacgcca
     9061 aaacgggtga tctgctcatc cgttgctgcc tggcgcgaaa tgacttcact gaacagctgg
     9121 cgggtgttga agtggggaac tacgccgtga aaaacaggaa aatgaaaccc aggacgaaac
     9181 ccagactcac tcaattccat tttgacttgt tgctcaatgg ggcgaataac atcggttaac
     9241 acccggcaaa gggtggactc aagctcggca aagcgcagcg taaagtcgcg actgatggtg
     9301 ttctgcgccg tctgtaacag tgtctcacag cgggtacgca tctcacttaa cgactccgaa
     9361 tcatcctgaa acaacgcggc taactgcgca ttcaccgcat cttgctgttg acgcagaaag
     9421 tggttggtgg aggtcagggc caactcgatt tcatgtttaa tctcgccgct cacctgcgcc
     9481 tgattgagtt gcaatagctg caaactttct tcgacctgat ggatattttg ctgcaattgt
     9541 tcacaagcga cgtttaaccc gtgcgcacga aaatccaggt attcccgcgc ctgctgcgcg
     9601 taattcaaca gtttatgcgc ggcagatcgc agagcataca acgaggcgtt agcgtaagcg
     9661 gcataaagca acgcctgaat tggctgggcg aacagcgaat cttcccacaa ctgatcggca
     9721 gcatggcgaa tatgttcgag gtccgccaga tcggcatgac gccagcgcct gccgagcgcg
     9781 gcatgggcaa aatcttccac ccagcgttgt tgctctggcg cgggtaactt accgttgttg
     9841 gctaactcat ggcgcgcccg attcgccagg tagccccaca tcgacgacac cggaaatatc
     9901 tgctgtggcg taatacagcc tttcatcagc gttccggaaa tcagtgcccg cacctggtcg
     9961 gcgtcgtcac tgttacgatc ctgttgatcg aacttattga ccagcacata caacggcacc
    10021 gattgcccca ccgccaaaat tgcctcacgg acctcttcat cggagatcga tttcagttgc
    10081 gtataatcca gcaccgccag taccgccgag gcgcgtgcca gctgctggtt aagcattttt
    10141 tgcagatgcg gttgcccggc ttcatttggc ccgggggtat ccagtaacgt caactgaccg
    10201 ggataactct ccagccccgc cagatggaca aactccactt caatcacggg aatatgctca
    10261 atggcggcgt aagcagaaaa aggaaaatcg acgtccagcg ccttcgccag tcgcaccaaa
    10321 tcattcaaac ttttcagaca atgaaaaata ggttgagcac ccagataata tttttcgaaa
    10381 gcgatgccat tttcgatccg ctgcataagc gcacgcatat ctttatctat ttccagcaca
    10441 tcggtcagat gcttaatatc gcaatcacgc aggcgctgtt gtaattgttg aattaaatgg
    10501 tcgattggcg cgacatgtga aaaatgcagt accggttctt tttgcccggg cgtatggcga
    10561 ataagcgttg gcagcgcagt cattgggcga ttgcgattag gcagaacctc cgtaccaaca
    10621 atggcattaa tggtggttga tttccctgct ttcatggtac cgacaatcgc cagcaccatt
    10681 tccagctggg aaattttacg caactcatta ttcagcatcg cgtgacgttc ggcgatatta
    10741 ggctgactcc agggtaaagc cagttgtggc gcatcgtctc cgggtacaga gagaggcatt
    10801 ttttccagta actgcaactg ttggcgagaa agctgtaaca ggcgttcagc ctcctgactt
    10861 aactcataca gggtctgtgt gtacatagaa aattcttcct taaagcaaat tttattactt
    10921 ttatttagcc agattgcttt tgagttctgt tttcggcttt tataattact gcaagaaata
    10981 attttatatt tagagtgtta ttttttatta gaataaataa cgtcttccga tacgtttaaa
    11041 acgtcagaaa gataaaaaat at
//
LOCUS       DAZFFE010000126        11011 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFFE010000126 DAZFFE010000000
VERSION     DAZFFE010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.126"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            74..1207
                     /gene="ompN"
                     /locus_tag="ACHJ69_004052"
     CDS             74..1207
                     /gene="ompN"
                     /locus_tag="ACHJ69_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO5016929.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSTNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1348..1782
                     /gene="uspF"
                     /locus_tag="ACHJ69_004053"
     CDS             1348..1782
                     /gene="uspF"
                     /locus_tag="ACHJ69_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO5016930.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1871..2471
                     /locus_tag="ACHJ69_004054"
                     /pseudo
     CDS             1871..2471
                     /locus_tag="ACHJ69_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012016515.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
     gene            complement(3698..3784)
                     /locus_tag="ACHJ69_004055"
                     /pseudo
     CDS             complement(3698..3784)
                     /locus_tag="ACHJ69_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450687.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            3826..4056
                     /locus_tag="ACHJ69_004056"
                     /pseudo
     CDS             3826..4056
                     /locus_tag="ACHJ69_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            4108..5043
                     /gene="ttcA"
                     /locus_tag="ACHJ69_004057"
     CDS             4108..5043
                     /gene="ttcA"
                     /locus_tag="ACHJ69_004057"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO5016931.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLETLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(5172..6545)
                     /gene="dbpA"
                     /locus_tag="ACHJ69_004058"
     CDS             complement(5172..6545)
                     /gene="dbpA"
                     /locus_tag="ACHJ69_004058"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO5016932.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDVSLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVICFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQSVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLSWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(6575..6748)
                     /gene="ynaL"
                     /locus_tag="ACHJ69_004059"
     CDS             complement(6575..6748)
                     /gene="ynaL"
                     /locus_tag="ACHJ69_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO5016933.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(7023..8006)
                     /gene="zntB"
                     /locus_tag="ACHJ69_004060"
     CDS             complement(7023..8006)
                     /gene="zntB"
                     /locus_tag="ACHJ69_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO5016934.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(8020..8067)
                     /gene="ydaN"
                     /locus_tag="ACHJ69_004061"
     CDS             complement(8020..8067)
                     /gene="ydaN"
                     /locus_tag="ACHJ69_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO5016935.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            8261..9493
                     /gene="dgcM"
                     /locus_tag="ACHJ69_004062"
     CDS             8261..9493
                     /gene="dgcM"
                     /locus_tag="ACHJ69_004062"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO5016936.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSEARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRNEEETALSCRLVLRKLTEAEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSMAKVSISSLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(9514..10077)
                     /gene="smrA"
                     /locus_tag="ACHJ69_004063"
     CDS             complement(9514..10077)
                     /gene="smrA"
                     /locus_tag="ACHJ69_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO5016937.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPARNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(10272..>11011)
                     /locus_tag="ACHJ69_004064"
     CDS             complement(10272..>11011)
                     /locus_tag="ACHJ69_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5016938.1"
                     /translation="FKDKNFTLGCFFSFSGGVGIFSTVYLIPVFLGQVRGLNAEEIGF
                     AVCTTGIFQLFSVPFYFWLSKKINLRWLLMAGLGGFVFSMYLFTPITHEWGWQELLFP
                     QAIRGISQQFAMAPIVTLTLGGIPKERLKLASGVFNLTRNLGGAIGIALCGSILNNRT
                     NFHFSRMGEKMVSVPHAVNDFISRSALFFNRSGSDQTSEILASTKLLSQLMLREAQTM
                     AFSDTFLLISGLLFIAFLLVPAMDKSS"
BASE COUNT         3060 a         2675 c         2385 g         2891 t
ORIGIN      
        1 cttctccttt aggatgtttt cactccccaa aatggggatg aaaggcaaat aaaataacta
       61 aaggatttat tcaatgaaaa gcaaagtact ggcactctta attcctgccc tgctcgctgc
      121 aggtgctgca catgcagccg aagtttataa taaagacggc aacaaattag atctgtatgg
      181 caaagttgat ggcctgcatt atttttctga taattcagcg aaagatggcg accagagcta
      241 tgctcgtctg ggttttaaag gcgaaaccca aattaacgat caactcactg gctacggtca
      301 atgggaatac aatattcagg caaacaacac cgaatcttca acaaaccagt catggacccg
      361 tctggcattt gccggactga aatttgcaga ttacggttct ttcgattacg gacgtaatta
      421 tggcgtaatg tacgacatcg aaggctggac tgatatgcta cctgaatttg gcggtgactc
      481 ttataccaat gcagacaact ttatgactgg tcgtgccaat ggcgtcgcga cttatcgtaa
      541 tactgatttc ttcggtctgg taaatggtct gaacttcgcg gtgcagtatc aaggtaacaa
      601 cgaaggtgcc agtaatggtc aggaaggcac caacaacgga cgtgatgttc gccatgaaaa
      661 cggtgacggc tggggtcttt ccacaacata tgatttaggc atgggcttta gcgctggtgc
      721 ggcatacacc tcttctgacc gcaccaatga ccaggttaac catactgcgg caggtggtga
      781 taaagcagac gcgtggactg ctgggctaaa atacgatgct aacaatattt acctggcaac
      841 catgtattca gaaacgcgta atatgacccc gtttggcgac agcgattatg ctgttgcaaa
      901 caaaacccag aactttgaag tcactgcaca gtaccagttt gattttggtc tgcgtccggc
      961 agtctctttc ctgatgtcta aaggccgtga cctgcacgct gccggtggtg cagacaaccc
     1021 ggcaggtgtt gatgataaag atctggttaa atacgccgat gttggcgcga cttactattt
     1081 caataaaaac atgtccacct atgttgacta taaaatcaac ctgttggatg aagatgacag
     1141 tttctacgct gccaacggca tctctaccga tgatatcgtc gctttaggtc tggtttatca
     1201 gttctaaatc cccctgcccg ctgttttggc gggctttttc tgcttattct tcctcctttg
     1261 atctaaatta aaaatgtgaa ctccgtcatt acacaaaaag tgtcatctgg cgttacactt
     1321 tatgcggata ctaaaacggg aggttttatg aacagaacga ttcttgtccc tatcgatatt
     1381 tccgattcag aattaactca acgcgtgatt agccacgttg aggcagaggc aaagattgat
     1441 gatgcagagg ttcatttcct gacggtaata ccttcgctgc cctactatgc ctctctgggt
     1501 ttagcctatt ccgcagaatt accggcaatg gatgatctga aagcggaagc caaatcgcaa
     1561 ctggaagaga tcattaaaaa atttaaactg ccaaccgaca gagtgcatgt ccatgttgaa
     1621 gaaggctcgc ccaaagaccg cattctggaa ttggcgaaga agatccccgc tcatatgatc
     1681 atcattgctt cccatcgacc ggatatcacc acttatctgc tcggttctaa cgccgcagct
     1741 gtagtgcgtc acgcagagtg ctccgtgctg gttgtgcgct gacactaacg cccgcacatt
     1801 gctgcgggct ttttgattca tttcgcaaat gtgctgacat tttcccctct aatcattaca
     1861 atttaaattt atgaattatg cattgataat cgctattata atatcgttcc cctgtgcatt
     1921 attatcagtt ttcctggtat taaaaggctg ggcgctaata ggagatgcaa tgaaccacgc
     1981 aatgtttcct ggcatagttc tagcctggat tataggtatg ccgctgggaa ttggtgcatt
     2041 tattgtagga ctgtcttgtg caattgcaac tagctatctg aaagataata gcatcataaa
     2101 gaaagatacc attataggta tcgttttttc aggaattttt gtcttgacta tcaacaggca
     2161 caagtcagcg gattacagat atcattatta cattacggtt tactctgcat ggtatcgcct
     2221 accatttttg ccacattaaa agcagtcgaa attattctgt ctatatacta ttaatagctc
     2281 cagggtctat tgctgtcctc atcacgcatc gctttcataa ggcattactt atcgccgtaa
     2341 cgatatcaat aacaatatca ggtgtatggt tattatattt tttcgataga gccccagcgc
     2401 cgacaatcgt cctcttattt gctgtagcat ttcttttcgc gcttataata atcaattttc
     2461 ggaaacaata atctcaacag tagaataaag aggcaaatca atcattacat atataagatg
     2521 gtgctaatat tgcaccgcct ttaaattgaa ctttatcctt tcacccttac acaatttaag
     2581 gaatgcagat gactatttta tcagcaccca gcctttttcc tggtaatcga aaacttgaaa
     2641 atcattaact ctaagaaatt ctgccaagtc gttgaaatta ctttaaatta gactcgaaca
     2701 aatcaatccc tgatgggact tttacttatc aatttaatcg tgtagatatc gacctccatc
     2761 tgcccgcctc agattcgtac ctggtggagg tcttttttcg ttacctcttt ccccctttag
     2821 ttcagtataa aatcatcaaa ctaatgctgt tcacactatt tacctccagc attctatata
     2881 aataatagaa ataaaaaccc caatacatag tgcattggca tgaccaaaca tcaatgccat
     2941 tatactcgcc tctgttaaat actcgctagt taatatgata ggcacgcatg atataacaat
     3001 ccatatgcta atacataaat acatcagtat ttttctcata gtcagtccct gtgattaaca
     3061 atcaaggcat agttaagcat atttcacatt caaacgtaaa cattattaat aacattatgt
     3121 caatcgaact gacatcacga ttcaagccct cttcagactg aaggttagag cagcgtagaa
     3181 catttcaaaa caatacacta aaaaaatact tgaacaaaaa acttaattct tttttattgt
     3241 gaactttagt aattttgtca tgcaactctt tattacattt aggcctatct tatttcatgg
     3301 caacgcagat acaggaccat ttcttgtgct atggataagt aataagctag ttacatctta
     3361 ttaagagaac catttttaca tatcaagata tcatcaccac ccacaatgaa ttaagtattt
     3421 ttcttattct ttagattaga tacgtaatga atccctaact ccattcgggt gtcatggttc
     3481 tttcatttga aatacattca tatccaatgt aatatttcca tgcgcaaata taaccaccaa
     3541 ccatacccat catttattac agagaattat tttattcacc gaaaagtttc acaaaatatc
     3601 ccctgataga aaactaaaaa acactgcata atatcgctat tctggcactt gcgaaaaatt
     3661 atattctgca cttgttgtag cgttctattt ctccgcctgg caacgaatgc acaatgtccg
     3721 ccacagtttc tgttctaatt tcttcctgtg ttaccgcatc ctgtacgggt agcgcaacac
     3781 cagcatgcta agcaaggctt tcccatcaga tttcactacg actatttggg agcgagatgg
     3841 aatgcagcag taaaacgtgc tggtattcgc cgccgtaatc cgtaccatac gcgacatact
     3901 tttgcctgct ggctgttgac ggcaggagcg aacccggcat ttatagccag ccagatgggg
     3961 catgaaactg cgcagatggt gtatgaaatt tacggtatgt ggattgatga catgaacgac
     4021 gaacaggtag ccatgttgaa tgcgcggtta tcgtagttgc aaagtttgcc ctcaatatgc
     4081 cccacttagt accagagaac tgaaataatg caagaaaatc aacaaattac aaagaaagaa
     4141 caatacaacc tgaacaaatt acaaaaacgt ctgcgtcgta acgtgggcga agccattgct
     4201 gacttcaata tgattgaaga aggcgatcgc atcatggttt gcctctccgg gggtaaagac
     4261 agctatacca tgctggagat tctgcgcaat ttgcagcaaa gcgcgccaat caatttttcg
     4321 ctggtggctg ttaacctcga tcaaaagcaa ccgggcttcc cggaacacgt tctgcccgag
     4381 tatcttgaaa cgctgggcgt tgagtacaag attgttgaag agaacactta cggtatcgtg
     4441 aaagagaaga ttccggaggg caaaaccact tgctcactgt gttctcgtct tcgtcgcggt
     4501 atcctttatc gcaccgcaac ggaactgggg gcgacgaaga tcgcgctggg tcaccatcgt
     4561 gacgatatcc tgcaaacgct gttcttaaat atgttctacg gtggcaagat gaaaggtatg
     4621 cctccgaaac tgatgagcga tgatggcaaa catatcgtga ttcgtccgct ggcctactgc
     4681 cgcgagaaag atattcagcg ctttgccgat gcaaaagcgt tcccgattat tccgtgcaac
     4741 ctgtgcggtt cacagcctaa cctgcaacgt caggtgattg ctgacatgtt gcgtgactgg
     4801 gataaacgtt atccggggcg tatcgagacg atgttcagcg cgatgcagaa tgtggtgccg
     4861 tcgcatctgt gcgataccaa cctgttcgat ttcaaaggca ttacccacgg ttctgaagtg
     4921 gttaacgggg gtgatctggc gtttgatcgc gaagagatcc cactacaacc agcgggctgg
     4981 cagcctgaag aagatgaaaa tcagttggat gagttacggc tgaatgtggt tgaagtgaaa
     5041 taaccaggat agcgcccgat gcgcaagcgt atcgggctac tcttatggag gccggataag
     5101 acgcggccag cgtcacatcc ggcaatcccg aagaagatgt ttactcttgc accctgcaat
     5161 tcaacatttc attattttaa taaccgcacc cggcacgttt ttcctttaat cttcccgccc
     5221 tgtagctgtt tccatgcttt atgagcaaca gcctgacgca ccgcgacata aacatgcgcc
     5281 ggatgcacgg cgattttgcc aatatctgcg ccatcaagcc cgatgtctcc tgtcagcgca
     5341 cctaatacat cacccgggcg cattttggct tttttcccgc catcgataca caacgttgcc
     5401 atttctgctt ccagcggcac aatggaacta ttcgctggcg gcgtttgcca gctaagtttt
     5461 atctgcaaca tgtcagaaat gatattggcc cgctgtgctt cttccggggc acagaaactg
     5521 atcgccagac cgctatttcc tgcacgagct gtacgaccga tgcgatgtac atgaacttca
     5581 gggtcccacg ccagctcaaa gttcaccacc agctcaagcg attttatatc cagaccacgc
     5641 gcagcaacat cagtcgcgac cagtacacgg gcgctaccgt tagcaaaacg taccagggtc
     5701 tgatcgcgat cgcgttgctc cagatcgccg tgtaacgaca atgcactttg ccctacttca
     5761 ttcagcgcgt cgcagacaga ctggcaatct tttttggtat tgcaaaacac cacgcaagag
     5821 gatggctgat gcaagcttaa taaccgttgc aacagaggaa ttttgccttt gctggatgtc
     5881 tcataaaatt gttgttcaat aggtggcaga gcatctgttg agtcaatttc aatcgctaaa
     5941 ggatcgcgtt gcactcttcc gctgattgcg gcaatggctt ccggccaggt tgccgaaaac
     6001 agaagtgtct gccgagatgc tggcgcaaaa cagatgacat catcaatggc gtcgctaaat
     6061 cccatatcca gcatgcggtc ggcctcatcc atcaccagcg tattcaacgc atccagtgat
     6121 acagtgcctt tttgcaggtg atccagcaaa cggcccggcg ttgccacgat aatatgcggc
     6181 gcatgttgca acgaatcacg ctgcataccg aacggttgac caccgcacaa cgtcaaaatt
     6241 ttggtatttg gcagaaaacg cgccagccga cgcaattcac ctgccacctg atccgccagt
     6301 tcacgcgtag gacacagcac taaagcctgg gtttgaaata gcgacacatc aatttgctgt
     6361 aacaagccga ggccaaaagc cgccgttttg ccgctgccgg ttttcgcctg cacgcgaaca
     6421 tcttttccgg caaggatcgc cggaagcgcg gcggcctgca ccggcgtcat ggttaaataa
     6481 cccaactcat taaggtttgt gagttgggcg ggaggcaaaa cattcagggt agaaaaagcg
     6541 gtcacaatct attctcgtgg tcatcgacgc aaagtcagca ggcgcgtatc ctcgcagatc
     6601 tacgctcacg atgcgacaat ttaatcggtt cttcatcggg tggtgggtca ggcatgggtt
     6661 gcgggcgagg gatcggatcg ggtactggaa caggatcgcc aggaatcggt tcagggacag
     6721 gaatttgcaa ataaataagt gtcgtcatat ttccctccgg tcattgggtg gactcttaaa
     6781 gggtagacgc tgataaataa caggcaaaaa aaagccgact catcaaagtc ggcgtcgtac
     6841 gaatcaattg tgctatgcag taattcaaaa aaggaagtaa gacaatatgg agcgcaacgc
     6901 ccatcgcttg acgttgcatt cacctgcaag agagatattg ccctgaatgg gtagagagtt
     6961 tattgacttc gctcaaactt tacggcgttt ttgtatacag gcagccggaa aaattgcttt
     7021 tgttacaacc atttactacg atgcaaccat aaagcaacac caccaataag aacaactaac
     7081 agaatacaaa aaattgaaaa tccaaattgc catccgccgc cagggatccc accaaggttg
     7141 acgccaaata accctgtcag aaacgtactg ggtaaaaaga ccattgccat caacgacatt
     7201 gtataggtac gacgagctaa attttcctgc atcacctgag cgatttcatc cgccatcacg
     7261 ccagtccgtg ctatacaggc gtcgatttcg tcaagaccgc gcccaaggcg atcggcaata
     7321 tcctgcatcc gacggcgttg gtcatcgctc atccacggca aacgttcact ggcaagacga
     7381 gcataaacat cacgttgcgg tgccatatag cgacgcatca caattaattg tttgcgcagc
     7441 agagccagaa atccacgcgg tggaatttgc tgatcgagaa gattatcttc gaggtcgata
     7501 attttatcgt gcagctgctc gataaattcg ctggaatgat cggtcaacgc atcgcacaca
     7561 tccaccagcc accccccgca atcggtcgga ccagtgccct cttcaagatc gctcaccaca
     7621 tcgtccagcg ccagcacttt gcgttgtcgg gtcgaaacaa ttaaccgccc gtccatatat
     7681 acacgcatgg cgaccagctg atcggggcgt tcatcggtgc tgccgtttat acagcgcaat
     7741 gtaatcagcg tgccttcacc gagacggctg actcggggcc gtgtgctctc gcccgccagc
     7801 gcatcacgta cgttattggg aagcagcggt gttgtcgcca gccattgggc gctatcatgg
     7861 tgtacataat taaggtggag ccagcaggga tgcgcttcat caatcacatc tgtattttcc
     7921 agcggtttaa cgccgcctct accatccagc atccaggcaa atactgcatc cgggacatta
     7981 acgtccgatc ccttaatcgc ttccacagtg cctccatcat caacgcatta ttttgtagtc
     8041 tagccttctg gccctgttac gcaacatctc atcaccccat taccctgaaa tgattaataa
     8101 aattctgtct aaattgaata caaaaagcaa aatgcttttc cgtatacaaa ccgtgtgaag
     8161 tgttaaatag cgtctatcat tatcagaatt atctgatcat atgacgtggc ttttttgcga
     8221 tcggatagca acaaaaattg ataaaaataa tgggatctca atgattacac acaacttcaa
     8281 taccctggac ttactcacca gtcctgtctg gatcgtttcg ccctttgagg aacagttaat
     8341 ttatgccaac agcgaggcgc gactgttgat gcaagacctc acgtttagtc agctacgaac
     8401 cggtccctat tccgtctcct cacaaaaaga actgccgaaa tacctctccg atctgcaaaa
     8461 tcaacacgat attatcgaaa tcctcaccgt tcagcgcaat gaagaggaaa cagcattgag
     8521 ctgtcgactt gttttgcgaa agctgacaga agcagaaccg gtgattattt tcgaaggtat
     8581 cgaagcgccg gcaacgctgg gtttaaaagc cagtcgctcg gcaaattatc agcgcaaaaa
     8641 acaaggtttt tatgcgcgct tttttctgac taactctgca ccaatgttgt tgattgaccc
     8701 ctcacgagat ggccaaatcg tcgatgctaa cctcgccgcg ctcaatttct atggttataa
     8761 ccatgaaacg atgtgccaga aacatacctg ggaaattaat atgctcgggc gtcgcgtcat
     8821 gcctatcatg catgaaatct cgcatttacc cggtggtcat aagcctttga attttattca
     8881 taaactggcg gatggttcga ctcgtcatgt gcagacctat gccgggccga ttgaaattta
     8941 tggcgacaag ctcatgttat gtattgtgca tgatattact gagcaaaaac ggctggagga
     9001 gcagctggaa catgcagccc accatgatgc gatgaccgga ttactgaatc ggcgacagtt
     9061 ttatcacatt accgagccag gccaaatgca gcacctcgct atcgctcagg attacagctt
     9121 gttgctcatc gacaccgatc gttttaaaca cattaacgat ctctatgggc attctaaagg
     9181 tgatgaggtg ttatgcgccc ttgcccgcac tctcgaaagt tgcgctcgca aaggcgattt
     9241 ggtgtttcgt tggggaggcg aagagtttgt cttattgcta ccaagaaccc cactggatac
     9301 cgcgctttcg ctggctgaaa ctatccgcgt aagcatggca aaagtgagta tttcgagctt
     9361 accacgcttt actgtcagca ttggtgtggc gcatcacgaa ggaaatgaaa gcatcgatga
     9421 actgtttaaa cgcgttgatg atgctttgta tcgagcgaaa aatgatggac gcaaccgcgt
     9481 gctggcggca taagccacgg atgcgtctcg tgatcaacga ctgcgcttag cgtggcgctc
     9541 ccagttttct tgcttcgcct gcgctgtttt acgtagtgcg acgtaacacg ccccgctgcc
     9601 gccatgatgc ggtagcgcag tgcaatatgc ctgaacatca tcaaattcgg tcagccagcg
     9661 cgccacatag ctgcggacaa tattggcatg cgatttatca tcccgtcctt tgccatgaat
     9721 aatcagcacg ttacgcaaac catccgccag ggcttgttga atgaaactga acatcatttt
     9781 gcggcactct tccaccggct ggcgcaaaag attcaggctc gcctgttgcg gatatttacc
     9841 actgcgcagc ttatccagca ccccatgttg caacccttcc cgccgaaact ccagcggctg
     9901 acttagcggg atgatgtcga gaaatccggt ggtgaggaaa ttatcaagtt gcagcgtgtc
     9961 gatacgctgc ggcgcacgtt ggttacgcgc tggatgccag tggacatctg tagcacgttt
    10021 cagcggctgg acatcttcca tggcgtcaag aaacagcgat ttgtcgtcaa ggttcatgta
    10081 acctcctatg caccttcaca cgtcgtgatt cagacgggtt aattgttcat acttactgca
    10141 tcgttccctt cacagattga aacaatactc tacttcatac ctgtgagtat ctgcgcaggc
    10201 ggagcatttt atactagcct gttagcagta tcgaacagtt tagcccgagt ttaattcagc
    10261 acaggctccg atcaggatga tttatccata gcaggaacca acaaaaatgc gataaataac
    10321 agaccactga ttagcagaaa ggtatccgaa aatgccatag tttgcgcctc cctcagcatc
    10381 aattgactca ataatttggt tgaagccagt atttccgatg tctggtcact tccactgcga
    10441 ttaaaaaata aagcacttct ggaaatgaag tcattcaccg catggggaac agaaaccatt
    10501 ttttcaccca ttctcgaaaa atgaaaatta gttcgattat tgagaataga gccacacagg
    10561 gcaataccaa ttgcgccccc aagattacgt gttaaattaa atacaccaga ggctaacttc
    10621 agccgttctt ttggaattcc ccccagcgtt aatgttacga tgggggccat cgcaaactgc
    10681 tgcgaaatcc ctctaattgc ctgtggaaac agtaactcct gccatcccca ttcgtgggtt
    10741 ataggggtaa aaagatacat tgaaaacaca aatccaccca gccccgccat caacagccat
    10801 cgcagattta tttttttact taaccagaag taaaagggaa cagaaaataa ctggaaaata
    10861 cctgttgtgc aaacggcaaa acctatctct tcggcattta atccgcgaac ctgccccaaa
    10921 aacacaggta ttaaatatac agtagagaat atcccaaccc ctccagaaaa agaaaaaaaa
    10981 catcccagtg tgaaattttt atccttaaaa g
//
LOCUS       DAZFFE010000127        10968 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFFE010000127 DAZFFE010000000
VERSION     DAZFFE010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.127"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(243..488)
                     /locus_tag="ACHJ69_004065"
                     /pseudo
     CDS             complement(243..488)
                     /locus_tag="ACHJ69_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
     gene            885..1277
                     /locus_tag="ACHJ69_004066"
     CDS             885..1277
                     /locus_tag="ACHJ69_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016939.1"
                     /translation="MELTADLLKSLNNIRQNATIYVHAYHYTDDEKCVMAPRSSLPSL
                     ELTVLQSEKIIDLLLSGYGYIRHDGYVQGYGEKTVATFTIQEGKHHHFRTNLALNKTG
                     NIADNALNHWLHRIRKTDIIVLSVVLTE"
     gene            1337..1618
                     /locus_tag="ACHJ69_004067"
     CDS             1337..1618
                     /locus_tag="ACHJ69_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016940.1"
                     /translation="MKTNKKDTKWYIFYRENSGEDILLEMSSFKECLSASKELMTPSN
                     YMICIERNGERIKRWDREIIAGSNKWINCPPDNFEILGELITINRIIKK"
     gene            complement(1608..3401)
                     /locus_tag="ACHJ69_004068"
     CDS             complement(1608..3401)
                     /locus_tag="ACHJ69_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016941.1"
                     /translation="MEKVSIRKINRTEHFPSLNLPSDTSWVVWWYGFLKKNKRRGEQP
                     KVAITFREVQADGTLCDRYYEQECLLTDLSFFKIGSVWTRGRCVAEILMEEKIFNVDF
                     SYANGGWKINSLYQAYKEGVSLPYDFSDYQLKSPYDKNWLLEFLTDDADKVIIHPLEF
                     FYRCYGHSDELKRVILTYSGNELRNRIFSEAYVEGDKIFYIKLRKRMNKYDAPFASFF
                     ALDGFTRKVIDRIQRQLDVCEDRKIYLKVAPWFKGKARIKVKGFWLENNVTFLGLSVT
                     GVSLPAGYTINYDRDNSNLVDEKAEQGSRQAWSNVPVRHIKNPDEPFSVDNAFSPDSG
                     ASTLEVEASVFEFLDDGCELSPLRRKKAKYISVTKNESDLHSLYSVNEVVDEQNSIST
                     GYMSLADKNEPERLYMESKGIVRDMWNAMVYLRGAYSERIKSLDWYTPESSFLNTQEP
                     ELVAIPEFNESEVENDKISKSILNWPYLDVVNKKDIRGFLISRMLIDDNMIYFMEIQR
                     RVCNEETMNSFSEVEKFKGLVFTLDNECEIDKWVSLLAHESRFVKGILQKIVGKCPGT
                     AMTYKHSPAKNEPVACYSALLNALSKVGVTF"
     gene            complement(3556..4287)
                     /locus_tag="ACHJ69_004069"
     CDS             complement(3556..4287)
                     /locus_tag="ACHJ69_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016942.1"
                     /translation="MPIKNKLGFISMKVVFNVMDGFDKTFLPLEFSGFHGRNGYCYLR
                     VQIKHGFIVFSCAQLLNYYRTSVTNAIEQVREAAVNALFREGVLSYTQQKEFLDILKT
                     SQRVDKEIDSQLWDYINANSIWFEYYNHNESLFLNDHFHIASFEGNRNPLWKKTSLED
                     LERAYPEFDFVIHKHHLEKWINGGLSPENVKKTIKEKGWSNKMLAARWGCTEVWVSKI
                     INDENRKVQWNDAINGLPVISDNMI"
     gene            complement(4419..5105)
                     /locus_tag="ACHJ69_004070"
     CDS             complement(4419..5105)
                     /locus_tag="ACHJ69_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFO5016943.1"
                     /translation="MTTFIDHQKAFILLFNQTARYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVINMAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWGVACMMAQMQLGNVKALFENKPFITLSEPACGAGSMILAMADTLNRSGYPAY
                     RRMWVSETDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRILLTPGHYLGNWSYR
                     LRHVQEQAAA"
     gene            complement(5228..6174)
                     /locus_tag="ACHJ69_004071"
                     /pseudo
     CDS             complement(5228..6174)
                     /locus_tag="ACHJ69_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
     gene            complement(6282..6719)
                     /locus_tag="ACHJ69_004072"
     CDS             complement(6282..6719)
                     /locus_tag="ACHJ69_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD29 domain-containing protein"
                     /protein_id="HFO5016944.1"
                     /translation="MMNVKSVTEVDDAVVARVSDVLEFAFPGQKFNVLKVCDSGVYNM
                     INVSWLDGPTEAEVRFITRAFEGKNGLRFVHESRKFSNEFVQECIDRLRKKYGQSNVP
                     PDVRVARYWKNDLWKIKTDRFPGNIDVAINEMGAETSKYRKVV"
     gene            complement(6793..7131)
                     /locus_tag="ACHJ69_004073"
     CDS             complement(6793..7131)
                     /locus_tag="ACHJ69_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016945.1"
                     /translation="MRPDLNILPGDSGCSVWFYNGMSQPRLLAGSIAGLLTDVTITSN
                     YRGDVTSEIHEVVQEWLATGRGNLADLKEELWYYNLYINPSADELMNASRRYGLGHTT
                     WLKDFISNAV"
     gene            complement(7199..7558)
                     /locus_tag="ACHJ69_004074"
     CDS             complement(7199..7558)
                     /locus_tag="ACHJ69_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016946.1"
                     /translation="MYSRFITGYWHLQSQSALLAHLCQLEGELAILRAWQRLGITPVP
                     EDEDEPSPLYSILWDVGEALGWLLYDLEMENVPAPGTIFHEVLDRVMSLGHETEHSIW
                     ADSISTGKRYAAIPDAF"
     gene            complement(7669..8652)
                     /locus_tag="ACHJ69_004075"
     CDS             complement(7669..8652)
                     /locus_tag="ACHJ69_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HFO5016947.1"
                     /translation="MKTKKHTSCPVNKTPERDLYQLVTDRVIEAIEMGVAPWKRPWRS
                     VRTHSGDPMMPVNALTRSAYRGINVLLLWLAAEEAGYSSNCWLTYRQAQALGGQVRKG
                     EKSTMAVIYKSWHKQDEDENGNRIFDAEGNPVMVDVPVLKANPLFNVEQCDGLSLPEV
                     ATTVTENGANDTLNAVADRVLTMVNACGVKIHSLPQDRAYYTPVFDRIVLPLSSQFTS
                     AEDYWSTLLHELVHSTGHAKRLNREGIVSSGGSYGDERYAFEELIAEMGSAFLCAQLG
                     VTGNVQHENYIGAWLKILRADKKALFSACRQAREATEYLLSVHDTPAEKAA"
     gene            complement(8745..8882)
                     /locus_tag="ACHJ69_004076"
     CDS             complement(8745..8882)
                     /locus_tag="ACHJ69_004076"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016948.1"
                     /translation="MTFPTPTERFFFYLLHRVPASVAWVSDTLKTFDFERHSLVMLAL
                     W"
     gene            complement(8860..9339)
                     /locus_tag="ACHJ69_004077"
     CDS             complement(8860..9339)
                     /locus_tag="ACHJ69_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016949.1"
                     /translation="MNSLLILLYVVVCRACTILRVIAPVSSVRAWCEYQLEGMTILRD
                     AYSSLAKAAGMQNDAFEYLESIARHLGYESGGDQLKSGLFRPFLWHIRAKGHRLGAVM
                     EMAVWQAWKNGCNDVCNPWEDVYNPWERGWNDGWTECLDQRIAALLKAVCIDFSDAD"
     gene            complement(9440..9820)
                     /locus_tag="ACHJ69_004078"
     CDS             complement(9440..9820)
                     /locus_tag="ACHJ69_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016950.1"
                     /translation="MGLDIFFLGRDRVTDAVVSVPETREVGYFRKVNPLIAWFETHCG
                     PVENAVERPVSRTELEALLSDLECLTPENCREFFPTTEGFFFGSQEYDQYYWKDVEIV
                     KSWVRSTLDSFDFERKILLLWAWW"
     gene            complement(9991..10392)
                     /locus_tag="ACHJ69_004079"
     CDS             complement(9991..10392)
                     /locus_tag="ACHJ69_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016951.1"
                     /translation="MFIPLLLRADMHITPFFHLLRQCLFNQKRCVHPQPPWSAPQLRH
                     LYCTPWFINATGNLSVRACIVSTNGALRHCFEFREGSHSREYRYWFEALPLSVVRFNV
                     FTGRISARRTLSAASRMAAQLTSFRYRFGLR"
     gene            10812..>10968
                     /locus_tag="ACHJ69_004080"
     CDS             10812..>10968
                     /locus_tag="ACHJ69_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009645816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5016952.1"
                     /translation="MHLHTYQYNDKLPLQKHDKGIIMEVSVIFFWVIYALIVISFLKS
                     IITDLIKE"
BASE COUNT         3136 a         2814 c         2233 g         2785 t
ORIGIN      
        1 tttcaggtga taaaattcac gacctgataa aaaaactggc ccagtaaaca cggagagtta
       61 tttacaataa caactctgcc atcactgact tattaaaaaa gcgccacaaa atgtggcgca
      121 ttgtttcatt acactatttt tactactggc cccggggacc cgactgatgc tgatgccatg
      181 gcgcgttcgc ctggctgtgt gtccctgatc agacagagtt gtggctgctc atgctccgcg
      241 acctcccatg cctgactggc tacagttgtt gagccggcac cgtgactgac cagccctgga
      301 taaggcggat atgtctccgt tcttaccggt ttcagtatcc ccgcacagcc tgcaagaaac
      361 agcagtatgc tctgctggat cgcatgacgg taccgggatg catcattccc ttcaatccac
      421 tgaatcagta cgtcaagaca gactgactta ccggtgattt caaaacgatt tctgcaccat
      481 tcagccataa tcaatgctcc tctggtaaat gaagagggca ctgtcccgtg aggaacaatg
      541 ccctaacggg gtgaatggcg gtcgggttac accctccgcc agcggttcag attaagcaac
      601 cggttatcag gaataacgac ttgaggtagc ctgaaaaaag aaactgcaat ccagccagcc
      661 cataaactgc gacacgtctt acagaagatg gatgtgtctt taccatcaat attctcctgc
      721 ttcaacaaat gaagcaggga atatcgtcag aaagcaacag gtcgtgaaca aataactctt
      781 tttgtattct gtaaatttct cagaagagaa aaagaattaa ttgtcgccaa cgctttcaac
      841 aggtcgcatc atcaacagat gacaacaacc ggaaggacac cgcgatggag ttgactgcag
      901 acctgctgaa aagccttaac aatataagac aaaatgccac catatatgtt cacgcatacc
      961 actatactga tgatgaaaaa tgcgttatgg caccacgctc atccctgccc tctctggagc
     1021 taacagttct gcagtcagaa aaaattatcg atctgcttct gagtggttac ggttatatca
     1081 ggcatgacgg ctatgttcag ggatatggag aaaaaactgt ggcaactttc acaatacagg
     1141 agggtaagca ccatcatttc aggacaaatc tggcactgaa taagacgggc aatatagccg
     1201 ataatgcgtt gaatcattgg ctacatcgta tacgtaaaac agacatcatt gttctgtctg
     1261 ttgttcttac agaataaatt cagttattta ctggaaattt ataaggtctt tatagttatc
     1321 cgccgagaat acccgtatga aaactaataa gaaagacaca aaatggtaca ttttttaccg
     1381 tgaaaattca ggcgaggata ttttgcttga aatgtcatca tttaaggaat gtctttcggc
     1441 atccaaagaa ttaatgaccc catcaaatta catgatatgc atagaaagaa atggcgaaag
     1501 aataaaacgt tgggacaggg aaatcatagc aggctctaac aaatggatta actgtccacc
     1561 tgataacttc gaaatattag gtgagctaat aaccattaat agaattatca aaaagtaact
     1621 ccgactttag acaatgcatt aagcaatgcg ctataacaag caacgggttc attttttgcg
     1681 ggagaatgct tgtaagtcat cgctgtgccc gggcattttc ccactatctt ttgtaaaata
     1741 cctttaacaa atcttgattc gtgagctaac aaagataccc atttatcgat ttcacattcg
     1801 ttatcaagtg taaaaaccag acctttaaat ttttccactt cagagaacga attcatggtt
     1861 tcttcattgc acactctcct ctgtatttcc ataaaataaa tcatattatc atcaatcaac
     1921 atccttgata tcagaaaacc tcttatatct ttcttattca caacatcaag gtaaggccag
     1981 ttaagaatcg acttggaaat tttatcattt tccacttcgc tttcattgaa ctccgggatt
     2041 gcaacaagtt caggttcttg cgtatttaag aatgagcttt ctggggtata ccagtccaga
     2101 gacttaattc tttcagaata agctcccctc aaataaacca tagcattcca catgtcacga
     2161 acaatccctt ttgactccat atacaatctt tcaggttcat ttttatcagc taatgacata
     2221 tatcctgtgc ttatgctatt ttgctcatca acaacttcat ttaccgaata taatgagtgt
     2281 aaatcacttt cattttttgt aacggatata tattttgctt tttttctcct taaaggactc
     2341 agttcacaac catcgtcaag aaattcaaaa actgacgctt caacttcaag agtcgacgct
     2401 ccagaatcag gagaaaaagc gttatcaaca ctgaaaggtt catccggatt ttttatatgc
     2461 cgaactggaa cattactcca ggcttgcctg ctcccttgtt cagctttttc atcaaccagg
     2521 ttactgttat ctctgtcata atttatagta tatcctgcag gcaaagaaac acctgtaaca
     2581 gaaagcccaa ggaaagtaac attattttcc aaccaaaatc ctttaacttt tattctggcc
     2641 tttcctttaa accagggagc cactttgagg tatattttac gatcttcaca tacatctaat
     2701 tgacgttgta ttctgtcaat cactttccgg gtaaacccat caagggcaaa gaaagaagca
     2761 aaaggagcat catatttatt cattctcttt cttaatttta tataaaagat tttgtctccc
     2821 tccacgtatg cctcagaaaa aatcctgtta cgcagttcat ttcctgaata agtaagaata
     2881 actctcttca attcatcgga atgtccatag caacgataaa aaaactcaag cggatggatg
     2941 atcaccttat ccgcatcatc tgttaaaaac tcaaggagcc aatttttatc atatggagat
     3001 ttcagttgat aatctgaaaa atcataaggt aatgacacac cttctttata tgcctgataa
     3061 agagaattaa tcttccaccc tccatttgca taagagaaat caacattaaa tattttttcc
     3121 tccatcagaa tttcagcaac acaccttcct cttgtccaga cactgcctat tttaaaaaaa
     3181 gacaaatcag tcaacagaca ttcttgctca taatatctat cacaaagagt tccatcggcc
     3241 tgaacttccc gaaaggtaat tgctacttta ggttgttcac ctcgtctttt attctttttt
     3301 aagaaaccat accaccaaac aacccaggat gtatcagaag gaagatttaa tgaaggaaaa
     3361 tgctctgtcc tgttgatttt tcttattgat actttttcca tacatctgtc cctctaaaca
     3421 acagaactct tatctcacaa gcaaatgttg aagataaaat agttattcac ttaaaccaga
     3481 aacgctctga tgcgaactac atccagtata tattaataat cttcttgcct tatgctggtg
     3541 aagcatgatt acacatcaaa tcatattatc acttatcaca ggaagcccat taatagcatc
     3601 attccactga acctttctat tttcatcgtt aatgatttta ctcacccata cctctgtgca
     3661 cccccagcgc gcagccaaca tcttgttgct ccagcctttt tcttttattg tcttttttac
     3721 attttcagga ctaagtcctc cattaatcca tttttcaaga tgatgtttat gaataacaaa
     3781 gtcaaactca ggatatgctc tctctaaatc ctccagtgaa gttttcttcc acagtggatt
     3841 cctgtttccc tcaaaagagg cgatatgaaa gtgatcattc agaaacaatg attcattatg
     3901 gttatagtat tcaaaccata ttgaattagc atttatgtaa tcccataatt gcgaatcaat
     3961 ttctttatct actcgttgtg atgtttttaa tatatccaga aattcttttt gctgcgtgta
     4021 tgacaatacc ccctccctga agagagcatt aaccgcagcc tctctgacct gctcaattgc
     4081 atttgttact gacgtacgat aatagttcaa taattgagcg catgagaaaa caataaatcc
     4141 atgtttaatc tgaaccctta aataacagta cccattccgg ccatgaaaac cagaaaactc
     4201 caggggcaaa aaagtcttat caaaaccatc cattacatta aacacaacct tcattgaaat
     4261 aaaccccagt ttatttttta ttggcacttg caatccttga gtttaaaaaa caagagtaac
     4321 ctgtcaacac cacttacata accataaaaa aaataacaaa aattgaccca cagatgtaat
     4381 aacgccagtc ctgagactgg cgtagtgata aaaaatgatc aggccgctgc ctgttcctga
     4441 acatggcgca gacgatatga ccagtttccg aggtaatgcc cgggcgtcag cagaatccgg
     4501 cgccgctcat tacacagcga gttaccaatc accacttctc cggccacacc acacagcgac
     4561 aattgtatat acgccatacc agctgccagc gggtcaatgt ccgtttccga cacccacatc
     4621 ctgcggtatg ccggataccc cgaacggttc agggtgtcag ccatagccag aatcatactg
     4681 ccggcaccac aggcaggttc actgagcgtg ataaagggtt tgttctcaaa caacgctttc
     4741 acattcccca gctgcatctg tgccatcatg caggccacac cccatggcgt gaaaaactgg
     4801 ctccggtatt tatcccccag ctccagctgc atgaacacgc tgcccagaaa gtcgcagaat
     4861 ccctgctcca gtccctcaat gacatgggca agcaaatgcg ccatgttgat aacatcctgc
     4921 ttttcataac cactgacgat tcgcaagtac ttctgctccc gtgcttcact gaactgaagc
     4981 cggttctcca gggcaatcac actgcagctg acaaagtcct caaacacctt atgacgtttg
     5041 tgataacggg cagtctgatt aaacagcaaa ataaaagctt tctgatggtc gataaaggtg
     5101 gtcatgtgct tctccataaa aaaacaggga acacatggcc ccggggagga acaagtgttc
     5161 cccttcgggc tgaaatgata gatgacagga aaaaacgggt gccgcgaatg tggcaccctg
     5221 aggttagtta ctggcctgca gccagcggaa ccggtaaagg cgcggcttct gtctgttctt
     5281 cagcgcgctc ctccaccaga tacagccggg gctgtggaag tttttccctc cccggccatt
     5341 cggcctgcgg gtcactgtcc gtatgttcac caccgtcata acagttgtaa ccctgtatgc
     5401 cccggacagg ttcgctgtac cagtgccgta tctcacagtc gaacaccttc gagatttcac
     5461 caaccagctc acctgaaggc ggataccagg gcgtgtctgt cagcagcacc agactgctca
     5521 tctcatcacg cacccagcgg acattgtgac ctgatggcca ctccacaccg tatatccggc
     5581 tgtaaaggct ggtcgtggtg gaaatgccct gcagcagtcc acctgaaccg ttcagctctg
     5641 tggccagacg gggggtatga ccatcagcat gtcacagggc tgcgcctgtt ccgggtaatc
     5701 cgacagccgg ctccagcagg cttcggcatc aatgtgttca ctcagtccga ccagaccaaa
     5761 ccagtcagaa tactgacaat gcattaacgt ggtgattatc tcccgggcat tatccggcag
     5821 attcccccag cggactgcgc caatacatga ctggtgataa aggcggtcaa tggtctttat
     5881 cgtctcttca tccagtacgg catcttttac cagcagcccc agccactgtt caaatgcctg
     5941 gttagccgga gatgacatcc ccgtaccatg acgaaccagc gccggatatg gcggatactg
     6001 cgtcgtccgg gtaggtttca ccagtccggc acaaccgatc agaaacagct gtatactctg
     6061 ctgtatggca tggcggtaac gcggtgtttc acatccttca atccactgcg tcagcacatc
     6121 caggcagact gacttaccgg taatttcaaa acgatttctg caccattcgg ccatattcaa
     6181 tgctcctctg ataaatgaag agggcattgt cccgtgaggt acaatgccct cacgggaaaa
     6241 gcggagttgt cacactccgc catcgtcaaa agtccgtccg gttatacaac cttccggtat
     6301 ttactggttt ctgcccccat ttcgttgata gctacgtcga tattgcccgg gaaacgatct
     6361 gttttaatct tccacaaatc gtttttccag taacgtgcca ctctcacatc tggcggaacg
     6421 ttgctctgcc cgtacttttt acgaagccgg tcaatgcact cctgcacaaa ttcattactg
     6481 aatttgcggg attcgtgcac gaacctcagc ccgtttttgc cttcaaacgc acgggtaata
     6541 aaccgcactt cagcctctgt tggtccatcc agccaggaca cattaatcat gttgtaaacg
     6601 ccggaatcgc ataccttcag cacgttaaat ttctgccccg gaaatgcgaa ttccagaacg
     6661 tcactgacac gggcaaccac ggcatcgtca acctcagtca cagacttaac attcatcatt
     6721 ctgctcctct gtatgaaaat cactgaggga acagccccgt gaggagtcgg gccctcacgg
     6781 gatggcggtg tgtcacaccg cgttactgat aaagtctttc agccaggtgg tatgcccgag
     6841 gccataccgc ctgctggcat tcataagttc gtctgccgac ggatttatgt aaaggttgta
     6901 gtaccagagt tcttccttta aatccgccag gttacccctg ccggtcgcaa gccactcctg
     6961 caccacttca tgaatttcag atgtgacgtc accgcggtaa ttcgatgtga tcgtaacatc
     7021 cgtcagtaac ccggcaatac tgcccgccag cagacgcggt tgagacatgc cgttatagaa
     7081 ccatacgctg cagccggaat ctccgggtaa aatattcaaa tcgggtctca tgaaatttct
     7141 cctgaaaaag cagggacccg tcccacaggg atgagtccct gtggggatgt aaaaaccgtt
     7201 aaaatgcatc aggtattgca gcgtaccgct tcccggtact gatactgtca gcccagatac
     7261 tgtgctctgt ttcatgtccc agagacataa ccctgtcgag tacctcgtgg aaaatcgttc
     7321 ccggtgcagg aacattttcc atctccaggt catacagcaa ccagcccaga gcctcaccaa
     7381 catcccacaa aatcgagtaa agtggtgaag gctcatcctc atcctccggc accggggtaa
     7441 ttccgagacg ttgccaggcg cgaagtatcg ccagttcacc ttccagctga cagagatgtg
     7501 ccagcaaagc ggactgactc tgaagatgcc agtaaccagt aataaaacgc gaatacataa
     7561 aaccccctgt ctgattcttt ctgatgaaag agcacaccgt ccgaaggaac gatgccctcc
     7621 gggcggtgaa caacagagtt cagattgttc acccgcgatt gttaagcatt acgccgcttt
     7681 ttctgccggc gtatcgtgta cggaaagaag gtattccgtt gcctctctgg cctgtcgaca
     7741 tgcactgaac agcgcttttt tgtcagcacg aagaattttc agccatgcgc caatataatt
     7801 ctcatgctgc acatttccgg tgacacccag ttgagcgcac agaaaggcac tgcccatttc
     7861 ggcgataagc tcttcgaacg cataacgttc atcgccatag ctcccaccgg aggaaactat
     7921 cccttcccgg ttaagacgtt tcgcatgacc ggtactgtgt accagctcat gcagtaatgt
     7981 ggaccagtaa tcttccgctg aagtgaactg tgacgagagc ggcaggacga tacgatcaaa
     8041 taccggggtg taatacgccc ggtcctgagg taaagaatga atcttcacac cgcaggcatt
     8101 caccatcgtc agtacacgat cagccacagc attcagcgtg tcgttcgcac cgttctcagt
     8161 gacggttgtc gccacctccg gcagcgacaa tccatcacac tgttcaacgt taaagagagg
     8221 attcgctttc agcaccggta catcaaccat aacggggtta ccctctgcgt cgaatatccg
     8281 gttgccgttc tcatcctcat cctgcttatg ccagcttttg tatatcacgg ccatcgtgga
     8341 cttctcgccc ttacggacct gcccccccag cgcctgagcc tgacggtacg tcaaccagca
     8401 gttactgctg taaccggctt cttctgcggc gagccacaac agcaacacat tgatcccacg
     8461 gtaagcgctg cgtgtcagtg cattaaccgg catcattgga tcgccgctgt gagtccgtac
     8521 tgagcgccat ggacgtttcc agggggctac gcccatttcg atggcctcaa tcaccctgtc
     8581 cgtcaccaac tgatacaggt cacgctccgg ggttttgttc acgggacatg aagtatgttt
     8641 tttcgttttc ataagagcac gctccataaa aaagatgcat gctccccgta cggtgaacat
     8701 gcaccatacg gttaaattaa taacgaaaat cagactggcc ggacttacca caacgccagc
     8761 atcaccagtg aatgacgctc aaaatcaaac gtcttcagcg tatcgctgac ccacgccacc
     8821 gacgcaggta cacggtgcag taagtaaaag aaaaaccgtt cagtcggcgt cggaaaagtc
     8881 aatacagacc gccttcagga gagcggctat acgttgatcc agacattcag tccagccatc
     8941 gttccatcca cgctcccatg gattgtagac atcctcccat ggattgcaga catcgttaca
     9001 gccattcttc catgcctgcc ataccgccat ttccatgaca gctcccagcc tgtggccttt
     9061 cgcacgtata tgccacagaa acggccggaa aagtcctgat ttcagttggt caccaccgct
     9121 ttcgtagccc agatgacgag caatactttc aagatattca aatgcatcat tctgcattcc
     9181 agccgcctta gccagcgagc tgtacgcatc acgaagtatc gtcatacctt ccagttgata
     9241 ctcacaccac gcacggacag acgacactgg tgcgattacc cgaagaatgg tgcaggcacg
     9301 gcatacaaca acgtaaagga gtattaacag actattcatg tgttcacctc agatacaaaa
     9361 aatcacggga acacacctgc cctacgggac acatgttccc gacagggtaa taaggttaaa
     9421 aacaaggaca gaaaaataat taccaccatg cccagagcaa cagtattttt cgttcaaaat
     9481 caaacgaatc aagcgtgctt ctgacccacg atttcacgat ctcaacatcc ttccagtaat
     9541 actggtcata ctcctgagaa ccaaaaaaga atccttctgt tgtggggaag aattcccggc
     9601 agttctccgg cgtaaggcat tcaagatcgc tcagtagtgc ctccagttcc gttctggaaa
     9661 ccggacgctc cacggcattt tcaaccggtc cacaatgcgt ctcaaaccag gcaatcagtg
     9721 ggttaacctt gcgaaagtaa cccacttccc gcgtttccgg taccgatact accgcgtcgg
     9781 taacacgatc acgcccaagg aaaaaaatat cgagtcccat aaacaatctc ctgataaaaa
     9841 cagcgggacc gccccccgaa cgggagcggt cccggacggg ttaaggaaaa ctcagatttg
     9901 tcatcgcgca cgcgtggtac ggtcagtcag atccgggcgt tgtgtgataa acctttcaat
     9961 ggtcatgacg accgttcaac aggcttggct tcagcgaaga ccgaagcgat agcgaaaaga
    10021 agtaagctgc gcggccatac gggaggcagc agagagcgtg cggcgagcgg atattcgacc
    10081 ggtaaagaca ttaaaccgca cgacagacag cggaagtgcc tcaaaccagt aacgatattc
    10141 acggctgtga gagccttcac ggaactcaaa gcagtgtcgc aatgcaccgt tggtggaaac
    10201 aatgcaggct ctgactgaca gattgccggt ggcgttaata aaccacggtg tgcagtacag
    10261 atgcctcagt tgtggagcgg accagggcgg ttgtgggtga acacaacgct tctgattaaa
    10321 cagacattgt ctgagaagat gaaagaaagg cgttatgtgc atatctgcac gtaacagaag
    10381 tggtataaac atcggaacct ccgaatgaaa aagacggaca ttccgccctg acggactgga
    10441 aaatccgtca gggtatgcca gcagaacacc tggcacagtc aagttgtcat aaatgcaacc
    10501 ggaaaaaccg gctggaatat caacgtacct caatcaccag tagtgatatc cgtttccaga
    10561 ggctaaacgg atgtaccgcc ataaaggcat cctctttcag gctacgagga tattgcaatc
    10621 acccgaaggc gtccctctca gatcaccggg acattggatg gtcgccggag cgacagttaa
    10681 tgcagatgca ggttaacaaa aagggaaatc tgccgtcaac catatgaaca tgttgatttc
    10741 attatgaatg tgttgtttta atataaatta atcggtaacg tcataaaaag aatataagaa
    10801 acagataaag catgcattta cacacatatc agtataatga taaattacct ctccaaaaac
    10861 acgacaaagg tataattatg gaagtttccg ttatattttt ctgggttatc tatgctctta
    10921 ttgttatctc attcttaaaa tcaataataa ccgacctaat aaaagaaa
//
LOCUS       DAZFFE010000128        10879 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZFFE010000128 DAZFFE010000000
VERSION     DAZFFE010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.128"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            155..1543
                     /gene="narU"
                     /locus_tag="ACHJ69_004081"
     CDS             155..1543
                     /gene="narU"
                     /locus_tag="ACHJ69_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO5016953.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKNIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1638..5378
                     /gene="narZ"
                     /locus_tag="ACHJ69_004082"
     CDS             1638..5378
                     /gene="narZ"
                     /locus_tag="ACHJ69_004082"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO5016954.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKSLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVT
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLSLKAEADKAGLSPAEF
                     TVQALKSGELRMACEQPDNGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVEHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5375..6919
                     /gene="narH"
                     /locus_tag="ACHJ69_004083"
     CDS             5375..6919
                     /gene="narH"
                     /locus_tag="ACHJ69_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO5016955.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQDEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6919..7614
                     /gene="narW"
                     /locus_tag="ACHJ69_004084"
     CDS             6919..7614
                     /gene="narW"
                     /locus_tag="ACHJ69_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO5016956.1"
                     /translation="MQILKVIGLLMEYPDELLWECKDDALALIRRDAPMLTDFALDLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLNCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     PLSSDSVTKQIRSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7611..8291
                     /gene="narI"
                     /locus_tag="ACHJ69_004085"
     CDS             7611..8291
                     /gene="narI"
                     /locus_tag="ACHJ69_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO5016957.1"
                     /translation="MIQYLNVFFYDIYPYLCGTFFILGSWLRYDYGQYTWRAASSQML
                     DKRGMVLWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLT
                     LIGGAGLLVRRLTNPRIRATSSGADILILAILLIQCILGLTTIPFSAQHPDGSEMMKL
                     VGWAQSIVTFQGGASTHLDGVAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8371..9264
                     /locus_tag="ACHJ69_004086"
     CDS             8371..9264
                     /locus_tag="ACHJ69_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO5016958.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKQNDDYRISLEQGTPGFEPPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGVIEVTVTIHDNQP
                     EKVTISGAAVILFHAEWAIEL"
     gene            complement(9360..10205)
                     /gene="nhoA"
                     /locus_tag="ACHJ69_004087"
     CDS             complement(9360..10205)
                     /gene="nhoA"
                     /locus_tag="ACHJ69_004087"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO5016959.1"
                     /translation="MTPILNHYFARINWLGAAAANIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEDKLVITRRGGYCFEQNGVFERVLRELGFNDRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLMPDIMQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10380..>10879
                     /locus_tag="ACHJ69_004088"
     CDS             10380..>10879
                     /locus_tag="ACHJ69_004088"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO5016960.1"
                     /translation="MSQFIPVELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVRGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDD"
BASE COUNT         2547 a         2870 c         2875 g         2587 t
ORIGIN      
        1 ttaatccaca ttctgccccc gcagttacgc tttcttatct attcttctaa tcagcacttc
       61 acctgattca tccttaattc taataactgg ctattgcgcc ttacctcctg ttttgtcttt
      121 ttttcattac caatgtgtgc atgtgaggaa caatatggca ctgcaaaatg agaaaaatag
      181 tcgttatctt ttacgcgact ggaaaccaga aaatccggcc ttctgggaaa ataaaggaaa
      241 gaatattgca cgaagaaacc tctggatatc agtcagttgc ctacttcttg ccttctgtgt
      301 ctggatgcta tttagcgcag ttactgttaa tctcaataaa atcggtttta atttcactac
      361 cgatcaactc tttttattaa ccgcattacc ctccgtttct ggcgcattat tgcgtgtccc
      421 ctactccttt atggtgccta tattcggtgg acgccgatgg acggttttta gtactgcaat
      481 cctgattatt ccttgcgtct ggctcggaat tgccgtgcag aatccgaata ctccttttgg
      541 gatatttatc gttatcgctt tgctatgcgg ttttgcaggt gcgaactttg cttcgagcat
      601 gggcaatatc agtttcttct ttccaaaagc caaacaaggg agcgctcttg ggattaatgg
      661 cggattagga aacttaggtg taagtgtgat gcagctggtt gcaccgctgg tcatttttgt
      721 ccccgtattt gcctttctcg gcgtcaatgg cgtaccgcag gccgacggtt cggtgatgtc
      781 gctggcgaat gccgcatgga tttgggtgcc attactggcg attgccacga tcgccgcctg
      841 gtcagggatg aatgatatcg ccagttcacg cgcctcaatt gccgaccagc tccctgtctt
      901 acaacgcctg catctctggc tgctgagcct gctttatctt gccaccttcg gttcgtttat
      961 cggtttttct gcgggttttg ccatgctggc aaaaacccag ttcccggatg tgaatattct
     1021 gcgcctggcg ttctttggcc catttatcgg tgccatcgcg cgatcggttg gtggtgctat
     1081 ttccgataaa ttcggcggcg tgcgggtgac gttgatcaac ttcattttta tggcgatttt
     1141 cagcgccctg ctgttcctta ccttaccggg cacaggctcc ggtaatttca tcgcatttta
     1201 cgccgtattt atggggctgt ttctgaccgc gggtctggga agtggttcta ctttccagat
     1261 gatcgccgtc atctttcgcc agataacaat ttatcgggtg aagatgaaag gcggtagtga
     1321 tgagcaagct caaagagaag ccgtcaccga aacggcagcc gctctgggct ttatctcagc
     1381 cattggcgca gtgggcggct tttttattcc gcaggcgttt ggcatgtcgc tcaatatgac
     1441 cggctctccg gtgggcgcga tgaaagtgtt tttaatcttc tacatcgttt gtgtgctact
     1501 gacctggctg gtttatggtc ggcggaagtt cagccaaaaa taagagatcg atgcctgaca
     1561 cgtcttatca ggctacaacc gtgtctcatc cgacaaggca tcagatccca attcattcct
     1621 gaagcaggag ttatgtcatg agtaaacttt tggatcgctt tcgctacttc aaacaaaagg
     1681 gcgaaacctt tgccgatggt cacggacagg tgatgcatag caaccgcgac tgggaggaca
     1741 gctatcgcca gcgttggcag ttcgacaaaa tcgtgcgttc cacccacggt gttaactgta
     1801 caggctcctg cagctggaaa atctacgtta aaaatggtct ggtgacctgg gaaatccaac
     1861 agaccgacta cccgcgcact cgccctgacc tgccaaatca tgaacctcgc ggctgcccgc
     1921 gtggcgcaag ttactcctgg tatctctaca gcgctaaccg cctgaaatac ccgctcattc
     1981 gtaaacgact gattgaactg tggcgcgaag ccctcaaaca acacagcgat ccggtactgg
     2041 cgtgggcatc gattatgaac gatccgcaaa agagcctgag ctacaaacaa gtgcgtggac
     2101 gcggtggctt tatccgctcc aactggcagg agctaaatca gctgattgcc gccgctaacg
     2161 tctggaccat caaaacttac ggcccggatc gcgttgccgg tttctcgccg attccggcga
     2221 tgtcgatggt ttcttacgcc gccggaacac gctatctgtc gttgttaggt ggcacctgtt
     2281 taagcttcta cgactggtat tgcgacctgc cgcccgcctc gccgatgacc tggggcgagc
     2341 aaaccgacgt accggaatct gccgactggt ataactccag ttacatcatc gcctgggggt
     2401 ctaacgtacc gcagacacgt acgccggacg cccatttctt taccgaagta cgctacaaag
     2461 gcactaaaac catcgccatt acccctgact actctgaagt ggccaaattg tgcgaccagt
     2521 ggctggcacc gaaacaaggc actgatagtg ccctggcaat ggcaatgggc catgtcattt
     2581 taaaagagtt tcatctcgat aatcccagcg attattttat caactactgc cgccgctaca
     2641 gcgacatgcc gatgctggta atgctggaac ctcgcgacga cggcagctac gttcccgggc
     2701 gcatggtccg cgcatctgac ctggtggatg gactgggcga aagcaataat ccgcagtgga
     2761 aaaccgtggc agttaatacc gcaggtgaat tggtagtgcc gaacggttcg attggttttc
     2821 gctggggaga aaaaggcaaa tggaacctgg aatccattgc cgccggtacg gaaaccgaat
     2881 tgtcgttaac cctgctcggt caacatgacg ccgttgcagg cgtggccttc ccctactttg
     2941 gcggcattga aaatccacat tttcgcagcg taaaacacaa tcctgtcctg gtgcgccaat
     3001 tgcccgttaa aaacctgaca ttagccgatg gtagcacctg tccggtggtc agcgtttatg
     3061 atttggtact ggcgaattac ggcctcgatc gcgggctgga agatgaaaac agcgcgaaag
     3121 attacgctga aatcaaacct tatacccctg cctggggcga gcaaattacc ggcgttccgc
     3181 gccagtatat tgaaaccatc gcccgtgaat ttgccgatac cgcccataaa acgcatgggc
     3241 gctcgatgat tattctcggc gcaggtgtta accactggta tcacatggac atgaactacc
     3301 gcgggatgat caatatgctg atcttctgcg gttgtgtcgg gcaaagcggt ggcggctggg
     3361 cacactatgt cggtcaggaa aaactgcgcc cgcaaaccgg ctggctgcct ctggcctttg
     3421 cgctcgactg gaaccgacca ccgcgccaga tgaacagcac ctcgtttttc tacaatcact
     3481 ccagccagtg gcgctatgaa aaagtcaccg cgcaggaatt gctctcaccg ctcgccgatg
     3541 ccagtaaata cagcggtcac ctgattgatt tcaacgttcg cgccgaacgt atgggctggc
     3601 taccttctgc gccgcagctg gggcgtaacc cgctctccct gaaagccgaa gccgacaagg
     3661 ccggattgtc acccgccgaa tttaccgtcc aggcactgaa gtcaggcgaa ttacgcatgg
     3721 cctgcgaaca gccggacaac ggcagcaacc atccgcgtaa cctgtttgtc tggcgttcaa
     3781 acctgcttgg ctcttccggc aagggccacg agtatatgca gaagtatctg ctggggaccg
     3841 aaagcgggat tcagggcgag gaactcggtg ccagcgacgg gatcaaacca gaagaagtcg
     3901 agtggcaaac tgcggcgatt gaaggcaagc tcgacctgct ggtgacgctc gacttccgca
     3961 tgtccagtac ctgtctgttc tccgatatcg ttctgcccac cgccacctgg tacgaaaaag
     4021 acgatatgaa tacctcggat atgcatccat ttattcatcc gctttctgcg gcggtcgatc
     4081 cggcctggga atcacgcagc gactgggaaa tctacaaagg tatcgctaaa gccttttcgc
     4141 aagtgtgcgt ggagcatctc ggcaaagaaa ccgacgtggt attacaacca ctgctgcatg
     4201 actccccggc agagctttca cagccgtgtg aagtgctcga ctggcgcaaa ggtgaatgcg
     4261 atctgattcc aggcaaaacc gcgccgaata ttgtggcggt ggagcgcgac taccctgcta
     4321 cctatgaacg ctttacctca ctcgggccat tgatggacaa acttggcaac ggcggtaaag
     4381 ggatttcgtg gaatacccag gatgaaatcg atttcctcgg taaactcaat tacaccaagc
     4441 gtgatggccc ggcacagggg cgtccgctga ttgataccgc gattgacgca tcagaagtga
     4501 ttctggcgct ggcaccggaa accaacggcc atgttgcagt caaagcgtgg caagcgctgg
     4561 gcgagatcac cggacgcgaa catacccatc tggcgctgca caaagaggac gagaagattc
     4621 gctttcgcga tattcaggcg cagccgcgta aaattatctc cagccccaca tggtctggtc
     4681 tggaaagcga tcacgtttcc tataacgcgg gatacaccaa cgttcatgag ctaattccgt
     4741 ggcgcacact gtcgggacgc cagcaactct atcaggatca tccgtggatg cgtgcttttg
     4801 gtgaaagtct ggtggcttat cgcccgccta tcgacacccg tagcgtcagt gaaatgcgcc
     4861 agataccgcc aaacggcttc ccggaaaaag cccttaactt cctgacgccg caccagaaat
     4921 ggggcattca ctcaacctac agtgaaaacc tgctaatgct gacgctctct cgcggtggac
     4981 cgattgtctg gatcagcgaa accgatgccc gagaactgac cattgtcgat aacgactggg
     5041 tggaagtttt taacgccaac ggcgcgctga cggcccgcgc ggtggtcagc caacgtgtac
     5101 cgccgggcat gaccatgatg tatcacgccc aggaacgcat tatgaatatt cctggttcgg
     5161 aagtaactgg catgcgcggc ggcattcata actcggttac ccgcgtttgc ccgaaaccaa
     5221 cgcatatgat tggcggttac gcgcagctgg cctggggctt taactactac ggcaccgtcg
     5281 gctcgaaccg cgacgaattc attatgatcc gcaaaatgaa gaacgttaac tggctggatg
     5341 atgaaggtcg cgatcaggtt caggaggcga aaaaatgaaa atacgttcgc aagtcggcat
     5401 ggtgcttaac ctcgataaat gtatcggctg ccatacctgt tcggtgacct gtaaaaacgt
     5461 ctggaccgga cgcgaaggca tggagtacgc atggtttaac aacgtcgaaa ccaaaccggg
     5521 cattggttat ccgaaaaact gggaagatca ggatgagtgg caaggtggct gggtgcgtga
     5581 tgtgaatggc aagatacgcc cgcgtctggg cagcaagatg ggcgtaataa ccaaaatttt
     5641 cgccaacccg gtagtgccgc agattgacga ttactacgaa cctttcacct tcgactacga
     5701 acatttgcat agcgcgccgg aaggcaaaca tattcccacc gcccgtccgc gttcactgat
     5761 tgacggcaaa cggatggaca aagtgatctg gggcccaaac tgggaagaac tgctgggcgg
     5821 cgaatttgaa aaacgagccc gcgaccgcaa cttcgaggcc atgcaaaagg agatgtacgg
     5881 gcagtttgaa aacaccttca tgatgtatct gccgcgcttg tgcgaacact gccttaaccc
     5941 cagttgtgta gcgacctgcc ccagcggcgc tatctacaaa cgcgaagaag acggcattgt
     6001 gctgatcgat caggataaat gccgtggctg gcgtttgtgc attagcggtt gcccatacaa
     6061 aaaaatctac ttcaactgga aaagcggcaa gtcagaaaaa tgtatatttt gttatccgcg
     6121 aattgagtcc ggtcaaccga ccgtgtgctc agaaacctgc gtgggtcgca tccggtattt
     6181 gggcgtgcta ctttacgacg ccgaccgcat cgaggaagcg gcgagcaccg agcgcgaagt
     6241 tgacctctat gaacgccagt gcgaagtgtt cctcgatccg cacgatcctt cggtgatcga
     6301 agaagccctg aaacaaggta ttccacaaaa cgtgattgac gctgcccagc gttcgcctgt
     6361 ctacaaaatg gcgatggact ggaaactggc gctaccgctg caccctgaat accgcaccct
     6421 gccgatggtc tggtacgttc cgccgctgtc accgattcag tcctacgctg atgcgggcgg
     6481 cttgccgaaa agcgaaggcg tgctgcccgc catcgaaagc ctgcgtattc cggtgcaata
     6541 tctcgccaat atgttgagtg ccggtgatac cggtccggta ctgcgggcgc tgaaacggat
     6601 gatggcgatg cgccactata tgcgttcaca aaccgtggaa ggcgttactg atactcgtgc
     6661 catcgacgaa gtaggcctga gcgtcgccca ggtcgaagag atgtatcgtt acctcgccat
     6721 tgccaactat gaagatcgtt tcgtcatccc gacaagccat cgggaaatgg cgggcgatgc
     6781 cttcgcagaa cgcaacggct gcggttttac ctttggcgac ggttgccacg gttcggacag
     6841 caaattcaac ctgttcaaca gtagccgtat cgatgccatc aacatcaccg aagtgcgcga
     6901 caaagcggag ggcgaataat gcagatcctc aaagtgatcg gcctgttgat ggagtaccca
     6961 gacgagttgt tgtgggagtg taaggacgac gcgctggcgc ttatccgccg cgacgcgccg
     7021 atgctcaccg attttgcact cgacctgctc aacgccccgc tactggataa acaggccgaa
     7081 tggtgtgaag tgtttgaccg cgggcgcacc acgtcgctgc tgctgtttga acatgttcat
     7141 gccgagtccc gcgatcgcgg ccaggcaatg gtggatctgc tggcggagta tgaaaaggtc
     7201 ggtctgcaac tgaattgtag ggaactgccc gattatctac cgctgtatct ggagtattta
     7261 agcgtacttc cggacgatca ggcgaaagaa ggattactca acgttgcgcc gatcctcgcc
     7321 ctgcttggcg gacgcttaaa acaacgcgag gcaccgtggt acgcgttatt tgacgccctg
     7381 ttgcaactgg caggaagccc tctgtcaagt gacagtgtca caaaacagat tcgcagtgaa
     7441 gagcgtgacg acacccgcca ggcgctggat gcggtgtggg aagaagaaca ggtgaagttt
     7501 attgaagata acgccacggc gtgtgacagc tcgccgctta atcaatatca gcgacgcttt
     7561 agccaggatg tcgcgccgca gtatgtcgac atcagtgcgg gaggtgggaa atgattcagt
     7621 atctgaacgt ctttttttac gatatctacc cgtacttgtg tggcacgttt tttatccttg
     7681 gaagctggct gcgctacgat tacgggcagt atacctggcg tgccgcctcc agccagatgc
     7741 tggataaacg cgggatggtg ctatggtcga acttgttcca tatcggcatt ctggggatct
     7801 tcttcggtca tctgttcggc atgttaacgc cgcactggat gtacgcgtgg tttctgccgg
     7861 tggcggtaaa acagcagatg gcgatgatcc tcggcggaat atgcggggta ttgacgctga
     7921 ttggcggtgc ggggctactg gtgcgtcgtt taacaaatcc gcgtatcagg gcgacgtctt
     7981 ctggtgcgga tatcctgatc ctcgccattt tgttaattca gtgcattctt gggttaacca
     8041 ccattccttt ttcagcacaa catccggacg gcagtgaaat gatgaagctg gtgggctggg
     8101 cgcaaagcat cgtcactttc cagggcggtg catctacgca tcttgatggt gtcgccttta
     8161 tattccgcgt tcatctggtg cttggaatga ccatcttcct gctcttcccg ttcacccgac
     8221 tggtgcatgt ctggagcgcg ccgtttgagt actttactcg tcggtatcag attgtgcgtt
     8281 cgcggcgtta attctcacgc gggccggata gcccttcgct ctccggccac ttgcaggcta
     8341 tgatagttct ccagcaacag gagaacatta atgaaaccgc aggtttacca cgtcgatgct
     8401 tttacctctc aaccgtttcg cggcaattct gccggtgttg ttttccccgc cgataatctc
     8461 agtgaagcgc aaatgcagct tatcgcccgc gagttaggtc attcggaaac tgcttttctg
     8521 ctgcacagcg acgacagcga tgtgcgcatc cgctacttta cgccaacggt tgaagttccg
     8581 atttgtggtc acgcgacagt agctgcgcac tatgtacgtg caaaggtgtt aggtttagga
     8641 aattgcacgg tctggcaaac atcgctggca ggaaaacatc gtgtgactat agaaaagcaa
     8701 aatgatgatt atcgtatttc gctggaacaa ggtactccag gctttgagcc accgctggaa
     8761 ggtgaaacac gtgcagcgat tatcaacgcg cttcatctca ctgaggacga tattctgcca
     8821 ggcttgccaa ttcaggtggc aacaacagga cactcaaaag tgatgattcc actgaagccg
     8881 gaagtggata tcgacgccct ttcgcctgac cttgctgcgc tgaccgcaat cagcaagcag
     8941 attggctgca atggtttctt cccgttccag atccgcccag gcaaaaacga aaccgatggt
     9001 cgcatgttct cgcctgcgat tggtattgtg gaagatccgg tgactggcaa tgccaatggc
     9061 ccgatgggtg catggttagt acatcacaac gtattgcccc acgatggcaa cgtgttgcgt
     9121 gttaaaggcc atcaggggcg cgcattaggg cgtgacggcg tgattgaagt gacggtgaca
     9181 attcatgata accaaccgga gaaagtcacc atttctggcg cggcggtgat tttgtttcat
     9241 gccgaatggg caattgaact ctgaaaccgt tgcctgatgc gcttcgctta tcaggtctac
     9301 aaccgggcta atgtaggccg gataaggcgt ttacgccaca tccggcaacc tgacataaat
     9361 tattttcccg cctccgggtg cgtatcaaac gccgccatca ccagcgctaa ctcatccaca
     9421 gtaaagccat gtttcgcatc atctacgccc agaccaaact gttcctgcat cacggcatat
     9481 aacgacgcca catctggtag atttcgctgc tcaactgcgt gcccattttc ataatgggta
     9541 aaatgaaaat tggtcagcgt tagcttgcca ccgtccggca aatggcgaca catcagcaaa
     9601 tgatggcgaa aatgcgactg cggccagtgc gccgaccaga aattgcccat cacataatcg
     9661 ctttgttgct gctcgcagag atcaaaacgg tacatcgact gccagtgctg atgatgatta
     9721 aactgcaata cccaatcatc accctcttgc aacaaccgat actctccgtg tggcgtggtt
     9781 tgcatgatat ccggcattaa acgaatcggc gcggttagcg tctgcccacc gaaaccgaca
     9841 tcagcaatcc atttttcccc ttccagttcc accaacagca aacgatgggt gcgcggcggt
     9901 aatgctggcg gatttgataa cactacgcga cctaacaagc tgcgatcgtt aaaccccagc
     9961 tcgcgtaaca cccgctcaaa cacgccattc tgctcaaaac agtaaccgcc acgacgggtt
    10021 atcaccagtt tatcttccag cgattgatca tcaagctgta tttccctcgg cagcaaaacg
    10081 tcgagatttt caaacggaat ggtgcaattg tgtttcaggt gcaatgcacg cagcgtatca
    10141 atattggccg cagcagctcc caaccagtta atacgggcaa aataatgatt cagaatgggc
    10201 gtcatcaggc gtttccctta gtggttttct caacttataa cctggaatag acgccgcact
    10261 ggctattttg tgcgatcgcg gacatgcatc acgttcgcaa atcgccaatc ccgttaattt
    10321 cgttacttat tgcgttacga gctacgcttt taaaacaccc ttcatagcga ggcgatatcg
    10381 tgagccaatt cattcccgtc gaattacacc atgccagccg tctgttaaat catggcccaa
    10441 ctgtcatgat caccagtttc gatgagcaat cccagcggcg taacattatg gcagcagcct
    10501 ggtcgatgcc cgtagagttt gaaccgccac gcgtggcgat tgtggtagat aaatcgacat
    10561 ggaccaggga gttgattgaa cgtaacggta agtttggcat cgttatcccg ggcgttgcgg
    10621 cgactaactg gacgtgggcg gtgggaagtg tgtcgggtcg tgaagaagat aaatttaatt
    10681 gctacggcat tccggtagtg agaggcccgg tgcttggttt gccgctggtc gaggaaaaat
    10741 gtctggcgtg gatggagtgt cgcctgctac ctgcaacttc cgcgcaagaa aaatacgata
    10801 cgctatttgg cgaagtggta tcagcagcgg cagacgcacg ggtatttgtc gaaggccgct
    10861 ggcagtttga tgatgataa
//
LOCUS       DAZFFE010000129        10801 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFFE010000129 DAZFFE010000000
VERSION     DAZFFE010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.129"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..707)
                     /locus_tag="ACHJ69_004089"
     CDS             complement(<1..707)
                     /locus_tag="ACHJ69_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFO5016961.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSMASDDSNNSW
                     NPAYNCFDRPGKKCISFTLHINGKDHDLLAVDKIHVDLNWRYLNEYLDHISANDEVLF
                     LKQGHEIIAKNQLAREK"
     gene            complement(884..1795)
                     /gene="gsiD"
                     /locus_tag="ACHJ69_004090"
     CDS             complement(884..1795)
                     /gene="gsiD"
                     /locus_tag="ACHJ69_004090"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO5016962.1"
                     /translation="MRLFNWRRQAALNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1798..2718)
                     /gene="gsiC"
                     /locus_tag="ACHJ69_004091"
     CDS             complement(1798..2718)
                     /gene="gsiC"
                     /locus_tag="ACHJ69_004091"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO5016963.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2736..4274)
                     /gene="gsiB"
                     /locus_tag="ACHJ69_004092"
     CDS             complement(2736..4274)
                     /gene="gsiB"
                     /locus_tag="ACHJ69_004092"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO5016964.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKNVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4294..6165)
                     /gene="gsiA"
                     /locus_tag="ACHJ69_004093"
     CDS             complement(4294..6165)
                     /gene="gsiA"
                     /locus_tag="ACHJ69_004093"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO5016965.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQEQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSILEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQVVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAVVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6152..7117)
                     /gene="iaaA"
                     /locus_tag="ACHJ69_004094"
     CDS             complement(6152..7117)
                     /gene="iaaA"
                     /locus_tag="ACHJ69_004094"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO5016966.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLV
                     AGESVLDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMECVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAPLDEKPKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7321..8556
                     /gene="moeA"
                     /locus_tag="ACHJ69_004095"
     CDS             7321..8556
                     /gene="moeA"
                     /locus_tag="ACHJ69_004095"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO5016967.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNTDGELEVTTTGHQGSHIFSSFSLGNCFIVLERERGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8556..9305
                     /gene="moeB"
                     /locus_tag="ACHJ69_004096"
     CDS             8556..9305
                     /gene="moeB"
                     /locus_tag="ACHJ69_004096"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO5016968.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            9388..10736
                     /locus_tag="ACHJ69_004097"
     CDS             join(9388..9868,9868..10736)
                     /locus_tag="ACHJ69_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1397 family transposase"
                     /protein_id="HFO5016969.1"
                     /translation="MKHSFEVKLAAVNHYLAGHAGIISTAKFFQLSHTSLSHWINLFL
                     LHGPRALDCRHKRSYSPEDKLCVVLYALGHSESLPRVAARFNIPSHNTVKNWIKGYRK
                     SGNEAFIRRRKEKSMTRSDDTHENEANMTPEEMKNELRYLRAENAYLKAMQEHLLEKK
                     PPGAGEKTKVTQSLRYGHCQSDLLKAAGLARSTLYYQLSLQKAKDKYADVKQLIASIF
                     HEHRGCYGYRRIHCELQKRGLKFSGKTVRKLMQQLGLKSPVRLKKYRSYRGNMGLAAE
                     NILQRQFKAEAPCEKWVTDITEFRAGGQKLYLSPILDLFNGEIVAWETACRPTEELVK
                     RMLNKGLESLAEGEKPLLHSDQGWHYRIKSYQSALADRGLVQSMSRKGNCLDNAVMEN
                     FFGHLKEEMYYRRDYRNVEELENAVNEYITYWNQKRIKLSPGGLSPVEYRTEYQKAG"
BASE COUNT         2701 a         2937 c         2694 g         2469 t
ORIGIN      
        1 aatttttcac gagcgagttg attcttagca atgatctcat gaccttgctt taaaaataga
       61 acttcatcat tagcgctgat gtgatcaaga tactcgttca gatatcgcca gtttaaatcg
      121 acatgaattt tatctactgc taacaaatca tgatctttgc cgttgatgtg tagcgtaaaa
      181 gagatacatt ttttacccgg acgatcaaag caattgtatg ccgggttcca gctgttgtta
      241 gagtcatctg aagccatcga ccacggtcgt ttcgatgggt taaaatcggc aaaaataacc
      301 cgccctgact cgtcgacaag cggtccattt acttgttcct gatgataaac aacgatttta
      361 tcattcgccc tccgcgaaaa gagataatat ttattatcat caagtaccag tcccaccgag
      421 tctaaaaatg gcatcatttc cattttcaga attacggcgc gttgaatctc ctctgtattt
      481 tttaataaat atagtgagtc ctttaatgtc agggccgaac gttcaatatc tttaaagaaa
      541 tagccaacgt ttttctgaat aagttcaaag cggctatcgg cacgttttac cgcgcgttcc
      601 gataccagtt gatagttaat gatgaaaaaa agaccaataa atgacagtgc tgccaataaa
      661 aataaaatta tcagccttat ctttatcttt tcgtataaac tcagcatgtt aatacattta
      721 acaaaataac tgtctgaacg ctgtgccgct gcaaaatagt cccttgctgc ggctttttaa
      781 ccaaattacc cgattctaca aaatcagcag aaagttacaa cacttgctta gggcaataat
      841 ttcctgtgag gtgattaccc tttcaagcaa tattcaaacg taactatcct ttaattttcg
      901 gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg gtcagaaaaa
      961 tagccagggc cggaaaaaca gcgacatgcg gcgcgataac catatccgct cgtgcctcat
     1021 tgagcattgc cccccactct ggtgtcggcg gctgcgcacc gaggccgaga aatgacaggc
     1081 tggcggcaga gataatcgag gtgccaatgc gcatggtgaa aaacaccacg atagaagaga
     1141 cggtcccagg caagatatga cgcaacaaaa tggtcatatc gctggcacca atactgcgtg
     1201 ctgactcgat aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc agccgggcaa
     1261 acgcggggat ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg cttcccaaca
     1321 ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc aaacaacaca tcgcaaatgc
     1381 gcatgatcag ccgatcccac cagccttcat aatatccagc cagcaagccc agcaacgtcc
     1441 cgatcgccgc accgataaac acggcaaaca cgcccgccgc cagcgagatt tgcgcaccaa
     1501 ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac accaaaccag tgctgcaaag
     1561 aaggtccgtt attcagattg tcataatcaa aataattttc ggcgtcatag ggagcgatcc
     1621 agcgtgcaaa aatggccacc acaatcaata aaataacgaa caatgcggcg gtcatcgcca
     1681 tatgctgacg gcgaaatcgt cgccagaatt catgccacgg tgtacgtacc tggtcaggtt
     1741 tgaccagtgg catggcgttt aacgccgcct gacgtcgcca gttaaatagt cgcatcctta
     1801 cttgtacctg atagccgggt taatggcggc gtaaagcaca tccaccacta agttgataag
     1861 aataaattcc agcgagaaaa gcagaatttc cgcctgaatc accgggtaat cgcgcatttc
     1921 aacggagtca acgagtaagc gtccaagtcc gggccagttg aaaactttct ccaccacgat
     1981 ggaaccaccg agcaagaagc caaactgtaa gcccatcatg gtcactaccg ggatcatcgc
     2041 gttgcgtagc ccgtgtttga ggacaaccca ggtttcgctc acccctttcg ccctcgcggt
     2101 acgcatataa tcttcgctta aaacgtcgac aaacgacgcg cgggtaaagc gcgccatcac
     2161 ggcggccact gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc aactgtctgc
     2221 tcccacggta ggcagccagc ccagttcaac ggagaatacc tgaattaaaa gcatccccag
     2281 agcaaatgcc ggaaacgaga tccccgacac cgcaatagtc atactcaatc gatccggcca
     2341 acggttacgc cagacggcgg cgataattcc cgccgccata ccaaatatca ccgcccagac
     2401 catactggtt atggtcagcc acagcgttgg cataaagcgg ctggcaatct catcggcaac
     2461 cggacgacgc gacaccatcg acaggccaaa atccccctgc acagcattgc tgatatagtg
     2521 ccagaactgg tgatacagcg gctgatccaa ccccagctgc tgacgcacca gttctataac
     2581 ctgcgcatca gcttcgggcc cggcaattaa tcgcgccgga tcgccgggca gcatgtggac
     2641 aaataaaaac accagcaccg agacgataaa cagcgtcgga atcaacccca gtaagcgttt
     2701 gataacgtaa ttaagcattc cactccctgc gttgcttatt gcaaatccgc gtcttcaaag
     2761 ctgaagccgg tgtctggcat gatccaaaaa ccggtcaggt ttttactgtg tgccgacacc
     2821 agtttttcta ccaccagcgg gatccacggc gactcttgcc agatgatatc ctgcgccgcc
     2881 ttatacaagc gggtcttttc cgccggatca ttagttttca gtgcctgagc caggaagtca
     2941 tccacctgtt tattgctgta aaacgcggta ttaaacagcg ttggtggcca gttctgcgag
     3001 gcaaacagcg gcgatagagc ccagtcggct tcgccggttg aagccgacca gccagtgtag
     3061 aacatccgca cgccgctctc tttttgccct ttaccttcaa cttctgccgc ccgctgtccg
     3121 gcatccatcg cagtcacctg ggctttaatc ccaacctgcg ctaactgctg ctgggtaaat
     3181 tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt actgaaaccg
     3241 ttgggatatc ccgcctcttt cagtaattcg cgcgctttca ctggatcgta aggccacggt
     3301 ttataacttt gcgcgtaggc gatactcggc ggtaccacac cagtagctgg cgttgcatag
     3361 cccgcgaagg ccactttcac cagcgccggg cggttaatgg cgtaattcag cgcctcacgg
     3421 accttcgggt tatcgaacgg cttttgcgtc acgttcatac tgatataacg ctgcataatt
     3481 gacggactgg ccatcaactc gatatttttg tttttctcca gcagtgcggc ctgctcgtag
     3541 ggaatgggga aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg ggtgttgtta
     3601 tccgccaccg gacgccaggt tatgctgtcc agtttgggca atcctggctg ccagtaaccc
     3661 gcgaattttt tcaccttcac aaaatcggtc tgattccagg tatccagttc atacggcccg
     3721 gttcccaccg gatgaaaacc aatctccttg ccatattttt ccagcgctgc cggtgaaatc
     3781 atcgcggtcg ccggatgggc aagaatatta ataaacgctg agaatggctg tttgagggta
     3841 atctttaccg ttgtcggatc gatcgcttcc gttttggcaa tattcttata caggttatag
     3901 cgtttaagat gattcgccgg atcgctggcc cggtccagat tcgctttcac cgccgctgcg
     3961 ttgaaatcgg tgccatcctg gaatttaatg ccttcccgca atttcacggt gtaagtaagg
     4021 ccgtcatcgg aaacggtata actctccgcc agcacgtttt tcagtttcat ctctttatcc
     4081 agaccgaaca gcccctggta aaacgatttc gctacggcct gagataacgt gtcatttgcg
     4141 tcatacggat cgagcgtggt gaagttcgag cccaccgcca ccaccacatt tttggcagcg
     4201 aatgcacaag atgccatcaa cgctgtcgca atgcccagcg ccactaaccc actacggtgt
     4261 acagctcttg ccattttatt ctccgcctga atgttatcta cgcatgaatg cgtattctga
     4321 ttgtggttgt gcgacgtaat gccccggccc gacgcattgc aacgagacga ctgcaacttc
     4381 ttcgccacgc agatgaatat tgctgggaag atcgtccgac agcagtacac gctgcggtcg
     4441 ttgtcgggac ggttcagcga ccggaactgc cgccagtaat ttacgcgtat aaggatgctg
     4501 cggattttca aagaccgcgc gccgtggacc aatttcaaca acttgcccga gatacatcac
     4561 cgccacacga tgactaatcc gctcaaccac ggccatatcg tgggagataa acagatacgc
     4621 aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc gaatagaaac
     4681 atccagcgcc gagacggctt cgtcggcaat gatcactttt ggattcaatg ccaacgcgcg
     4741 agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgagggtaac gccaggcatg
     4801 ttccggtaac agacccacgc gctccagcaa ccacgcaacg cgtgcagccg cttctttacc
     4861 tggcaataaa ccgtgtacgc gcagcggttc gagaatcgaa tcaccgatgg tctgacgtgg
     4921 gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcgcggcgca atgcctgaag
     4981 tttaccgggt gacaaggtat cgattcgctg accgttaaag ataatttcgc cgccttgcga
     5041 ttcgaccagg cgcagcaacg cccgcccggt ggtggattta ccgctgccag actcgcccac
     5101 cagcgatagc gtttcaccag gccagagatc aaaactgact ttctcaacgg catgcacttc
     5161 ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacca gattacgcac
     5221 ccgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atgggtggct cctgtttcgc
     5281 tggatgttca agcgatatca atgggaaacg tcggggataa tctaaccctt tcatcgcacc
     5341 aagttgcgga acagcagcta acagcgcacg ggtataagga tgttgcggtg catgaaaaat
     5401 ctgttcgacg ctacccgttt ccaccgcctc gccctgatac atcaccagta cccgatcggc
     5461 aatctctgcc accacaccca tatcgtgagt gataaagata acgcccatcg acatctcttt
     5521 ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca gcgcggtggt
     5581 tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa tcatcactcg
     5641 ctggcgcatc ccgccagaga gttgatgcgg ataacgtgaa agaatggttt gtgcctccgg
     5701 aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac gactggcgtt
     5761 ctgatgcaga cgaatagatt cagcaatctg ttcacccaca gtaaataccg ggttcagcga
     5821 tgtcatcggc tcctgaaata tcatcgccat atccgcaccg cgcacatggc gcatttgtgc
     5881 agcgctctgc tcgctaagtt caattacttc gcgactgcgc cgctgcaaca gcattttatc
     5941 gcactgcact aaaccgcccg cctgttccaa caggcgcatc aatgccagcg cagtcactga
     6001 cttaccggag ccggattcgc caacaattgc cagcgtctca ccacgttgca ggctaaaaga
     6061 gagattgcgg accgcagcta ttttctgctg ctcctgcata aaggcaatat tcagattttc
     6121 aaccgccagc acatcaccgg catcaagttc atcactgtgt ggcaacggtg tccccttttt
     6181 cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga tacattcctt
     6241 cggtgttaaa cggtagcgcg acattccctt catggtcgat agcgattaag ccaccgctac
     6301 cgccaagcgc agggagtttt tccattacta cccgctcgca ggcttccgcg agacttaatc
     6361 cgccgtaatc cattaacgcg gcgatatcat atgccgccag cgcgcggatg aagacttcgc
     6421 ccgtaccggt acaggaaacc gccacactgg cgttattggc atagcatccg gcccccacta
     6481 agggactatc gccaactcgt ccgggtaatt tattggtcat tccgcccgtg gacgtggctg
     6541 ccgccagatt gccgtctaaa tccaacgcca cggcccctac ggtgcccatt tttggttttt
     6601 catccagtgg cgcaccgcta tggtcgagga ctgttgcccc ttcctcgcgc gctgccatta
     6661 gttgttcata acgcaaaggt gtggagaaaa tctccggcga gacgcactcc atgccatgag
     6721 caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgggctt tgctccatca
     6781 ccagtcgggc ggcaagaacc ggattacgca gatgactaac gcccgccacc gcaccggctt
     6841 tcagggtgtt accatccatc acacaggcgt ccagttcatg ggtttcatca cgcgtaaaga
     6901 cagcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc gcttccgtca
     6961 ccacatccag cacactttcg cccgctacca gcattttctg cccggtttca acaatggcag
     7021 acaacgcctc gatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg ctaattgcac
     7081 ctgcgccacc atgaattgca atgactgctt tgcccatagt attcgtccat taagggtggc
     7141 tcgccacttt tgctataaat atcagaattt ttatgaatga ctcattcaat ttttgaatat
     7201 agaaagatgg atatggcatg taaaggcagg agccagcgct acgcccgagg tggcatacag
     7261 tctgtggcgt ttttctgaca taataggcaa attcgatttt gcctccgcag gagtgttttc
     7321 atggaattta ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat gctttctcgc
     7381 gttaccccac tgaccgccca ggaaacgctg ccactggttc agtgttttgg tcgtattctg
     7441 gcgagcgatg tcgtttcgcc actggatgtc ccggggtttg ataactccgc tatggacggc
     7501 tacgcggtgc gtttagccga tattgcctcc gggcaaccgc tgcccgttgc cggtaaatcc
     7561 tttgccggtc agccgtacca tggtgaatgg cctgcgggta cctgcattcg tattatgacc
     7621 ggtgcgccgg tgccagaagg ctgcgaagcg gtggtgatgc aggagcagac tgaacaaacg
     7681 gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg ccgtcgcggt
     7741 gaagatatct ctgcaggtgc ggttgttttc ccggcgggga ctcgcctgac taccgctgaa
     7801 ctgccagtga ttgcttcgct agggattgcc gaagttccgg tgattcgtaa agtgcgtgta
     7861 gcgctttttt ctaccggtga tgaactccag ttgcccggtc agccgctggg cgacggccaa
     7921 atctacgata ccaaccgtct cgccgtacac ctgatgttag aacagttggg atgcgaggta
     7981 atcaacttag ggattatccg cgacgatccc catgccctgc gcgccgcatt tattgaagcc
     8041 gacagccagg cggatgtagt gatcagttcc ggcggtgttt cagtgggtga agcggattac
     8101 accaaaacga ttcttgaaga gctgggggag atcgccttct ggaaactggc gattaaaccg
     8161 ggtaaaccgt tcgcgttcgg taaactttct aatagttggt tctgcggcct gccgggcaac
     8221 ccggtttcag cgacgctgac cttctatcaa ctggtacagc ctttgctggc aaaactaagc
     8281 ggcaataccg ccagtggcct gcccgcgcgc cagcgtgtac gcacagcgtc ccgcctgaaa
     8341 aaaacgccag gacgccttga tttccagcgc ggcgtgctgc aacgcaacac cgatggcgaa
     8401 ctggaagtga cgaccaccgg acatcagggt tcacatatat ttagctcctt tagcctcggc
     8461 aactgcttta tcgtgctgga acgcgagcgc ggcaatgttg acgtgggcga atgggtggaa
     8521 gtcgaaccgt ttaacgcgtt gttcggaggc ctgtaatggc agaactcagc gatcaggaga
     8581 tgctgcgcta caaccggcaa atcattctgc gcggttttga ttttgacggt caggaagcgc
     8641 tgaaggattc tcgcgtgttg gtggtggggc ttggtggcct cggctgcgca gcttcgcagt
     8701 atctggcaag cgccggtgtc ggcaacctga cgctgctcga cttcgacacg gtttcgctct
     8761 ccaatctgca acgccagacg ctgcacagtg atgccacggt cgggcaacca aaggtggaat
     8821 ccgcccgtga cgccctgacg cggatcaacc cacatatcgc gattacgcca gtcaatgcgc
     8881 tgcttgatga cgctgaactt gctgcgatga ttgcaaaaca cgatctggtg ctcgactgta
     8941 cggataacgt tgcagtacgt aatcaactga acgcaggctg ttttgccgcg aaggtgccgc
     9001 tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact tatcaggacg
     9061 gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga gaacgcgtta acctgcgtag
     9121 aagcaggcgt gatggcaccg ttaatcggcg taattggttc gttgcaggca atggaagcaa
     9181 tcaaactgct ggcaggttat ggcaaaccag ccagcgggaa aatcgtgatg tacgatgcga
     9241 tgacctgtca gtttcgcgaa atgaaactga tgcgtaatcc ggggtgtgag gtgtgcgggc
     9301 agtaactgcc acctccaggc cagataagac gcggcatgaa ctgcacccca aatgttggac
     9361 ataatctgac atctgaaggt gcagtttatg aaacattcat ttgaagtaaa acttgccgct
     9421 gtaaatcatt acctggctgg tcatgcaggc atcatttcta cggcaaaatt ctttcaactt
     9481 tctcacacca gcctctcaca ctggattaat ctttttcttc ttcacggtcc tcgggcactg
     9541 gattgcagac acaagcgtag ctattctcct gaagataaac tttgcgtggt tctttatgct
     9601 ctcgggcatt ctgagtctct accccgggta gctgcccggt ttaacattcc cagccacaac
     9661 acggttaaaa actggataaa aggctaccgc aaatctggta atgaggcctt tatcaggcgc
     9721 aggaaggaga aaagcatgac ccgttctgat gatacccatg aaaacgaggc aaacatgacg
     9781 ccggaagaga tgaagaatga gctgcgttat cttcgcgcag aaaatgcata tctgaaagcc
     9841 atgcaggagc atcttctgga aaaaaagcgc caggagctgg agaaaaaacg aaagtcaccc
     9901 agagcctgag gtacggacac tgccagtctg acctgttaaa ggccgcaggg ctggccagaa
     9961 gcacgctgta ctaccagctg tcactgcaga aggctaagga caagtatgct gatgtgaaac
    10021 agctcatagc ctccatcttc catgaacacc ggggatgtta tgggtaccgg cggatccatt
    10081 gtgagcttca gaaaagaggt cttaagttca gtggcaaaac ggtgcgaaaa ctgatgcaac
    10141 agctcggcct gaagtctcct gtgcgcctga agaaataccg gtcttaccgg ggaaatatgg
    10201 gactcgctgc agaaaatatc cttcagcgac agttcaaagc ggaagctccc tgtgagaaat
    10261 gggtgacaga tatcaccgaa ttcagggctg gcggacaaaa actgtacctg tccccgatac
    10321 ttgatttgtt taacggggaa atcgtggcgt gggaaacggc ctgcaggcct acagaggagc
    10381 tggtgaagcg gatgttaaat aaggggctgg aaagccttgc tgaaggggag aaaccgctgc
    10441 tccacagtga tcaaggatgg cattaccgga taaaaagtta tcagtccgcc ctggcggaca
    10501 gagggttagt gcagagcatg tcgcgcaagg gcaactgcct tgataatgcg gtaatggaga
    10561 acttctttgg tcacctgaaa gaagaaatgt actatcgccg ggactacaga aatgtagaag
    10621 agctggaaaa tgccgttaac gaatacataa cttactggaa tcaaaaaaga ataaaactca
    10681 gtcctggggg actcagcccg gtagaatacc ggactgagta tcaaaaagcc ggttaactga
    10741 aactgtccag gttttggggt tcagttcaaa acgccttatc cggcctaccg ttccgacacc
    10801 g
//
LOCUS       DAZFFE010000130        10801 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZFFE010000130 DAZFFE010000000
VERSION     DAZFFE010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.130"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(51..983)
                     /gene="melR"
                     /locus_tag="ACHJ69_004098"
     CDS             complement(51..983)
                     /gene="melR"
                     /locus_tag="ACHJ69_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO5016970.1"
                     /translation="MFQERESSMNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPH
                     IMPTSHWHGQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMA
                     IFNLPMHLFLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQL
                     AIDEIGLMLKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTL
                     NDVAEHVKLNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGF
                     RSSSRFYSTFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            1242..2597
                     /gene="melA"
                     /locus_tag="ACHJ69_004099"
     CDS             1242..2597
                     /gene="melA"
                     /locus_tag="ACHJ69_004099"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO5016971.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTILHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTVEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKAGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            complement(2594..3244)
                     /gene="yjdF"
                     /locus_tag="ACHJ69_004100"
     CDS             complement(2594..3244)
                     /gene="yjdF"
                     /locus_tag="ACHJ69_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO5016972.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDPWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITRAPEAITP"
     gene            complement(3367..5013)
                     /gene="fumB"
                     /locus_tag="ACHJ69_004101"
     CDS             complement(3367..5013)
                     /gene="fumB"
                     /locus_tag="ACHJ69_004101"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO5016973.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDIQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPEELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(5091..6431)
                     /gene="dcuB"
                     /locus_tag="ACHJ69_004102"
     CDS             complement(5091..6431)
                     /gene="dcuB"
                     /locus_tag="ACHJ69_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO5016974.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(7002..7721)
                     /gene="dcuR"
                     /locus_tag="ACHJ69_004103"
     CDS             complement(7002..7721)
                     /gene="dcuR"
                     /locus_tag="ACHJ69_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO5016975.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDSPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAVTIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(7718..9349)
                     /gene="dcuS"
                     /locus_tag="ACHJ69_004104"
     CDS             complement(7718..9349)
                     /gene="dcuS"
                     /locus_tag="ACHJ69_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO5016976.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MHSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVNVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVFAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            9530..9760
                     /gene="yjdI"
                     /locus_tag="ACHJ69_004105"
     CDS             9530..9760
                     /gene="yjdI"
                     /locus_tag="ACHJ69_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO5016977.1"
                     /translation="MDRALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            9772..10044
                     /gene="yjdJ"
                     /locus_tag="ACHJ69_004106"
     CDS             9772..10044
                     /gene="yjdJ"
                     /locus_tag="ACHJ69_004106"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5016978.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            10273..10569
                     /gene="ghoS"
                     /locus_tag="ACHJ69_004107"
     CDS             10273..10569
                     /gene="ghoS"
                     /locus_tag="ACHJ69_004107"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO5016979.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLSIMTVDDYFRQFYK"
     gene            10597..10770
                     /gene="ghoT"
                     /locus_tag="ACHJ69_004108"
     CDS             10597..10770
                     /gene="ghoT"
                     /locus_tag="ACHJ69_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO5016980.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
BASE COUNT         2675 a         2572 c         2790 g         2764 t
ORIGIN      
        1 gcttattatc gacggctaaa ctattttttt ggctgatact gatatcgtct ttagccggga
       61 aacgtctggc ggcgctgttg gctaagtttg cggtattgtt gcggcgacat gccgacatat
      121 ttgccgaacg tgctgtaaaa acggctactc gaacgaaagc ctgccgtcag ggcaatatcg
      181 agaatacttt tatcggtatc gctcagtaac gcgcgaacgt ggttgatgcg catcgcggta
      241 atgtactgtt tcatcgtcaa ttgcatgacc cgctggaata tccccattgc atagttggcg
      301 ttaagtttga cgtgctcagc cacatcgttg agggtcagcg cctgatcata gttttcggca
      361 ataaagccca gcatctggct gacataaaat tgcgcatggc gcgagacgct gtttttgtgt
      421 gtgcgcgagg ttttattgac cagaatcggt tcccagccag agaggctaaa tcgcttgagc
      481 atcaggccaa tttcatcaat ggcgagctgg cgaatttgct cgttcggact gtttaattcc
      541 tgctgccagc ggcgcacttc aaacgggcta agttgctgtg tcgccagtga tttgatcacc
      601 atgccgtgag tgacgtggtt aatcaggtct ttatccagcg gccaggagag aaacagatgc
      661 atcggcagat taaaaatcgc catgctctga caggttccgg tatctgttag ttggtgcggt
      721 gtacaggccc agaacagcgt gatatgaccc tgattgatat tcactttttc attgttgatc
      781 aggtattcca catcgccatc gaaaggcaca ttcacttcga cctgaccatg ccagtggctg
      841 gtgggcatga tatgcggtgc gcgaaactca atttccatcc gctggtattc tgaatacagc
      901 gacagtgggc tacgggtctg cttttcgtcg ctgctgcaca taaacgtatc tgtattcatg
      961 gatgactctc tttcctggaa tatcagaatt atggcaggag tgagggagga tgactgcgaa
     1021 caggcgcacg gttttattcc tctccctgga ggtgcgagga gagcggatga gaagatttgg
     1081 gtcgattatt cagcttttca ggtaatttat tcccataaac tcagatctat tgatgtgcca
     1141 cttaggatct gagttttcgg gaatgctcaa ccgggaaacc ggaggttttc tgcgatatcg
     1201 cctgccatga tgaagttatt caagcaagcc aggagatctg catgatgtct gcacccaaaa
     1261 ttacatttat cggcgctggt tcgacgattt tcgttaaaaa tattcttggt gatgtgttcc
     1321 atcgagaggc gctgaaaacg gcgcatattg ccctgatgga tatcgatccc acccgcctgg
     1381 aagagtcgca cattgtggtg cgtaagctga tggattcagc aggggccagc ggcaaaatca
     1441 cctgccacac ccaacagaaa gaagccttac aggatgctga ttttgtggtg gtggcatttc
     1501 agattggcgg ttatgaacct tgcacggtga ctgatttcga ggtctgtaag cggcatggtc
     1561 tggaacaaac cattgccgat acgctggggc cgggcggcat tatgcgtgcg ctacgtacca
     1621 ttctgcacct gtggcagatt tgcgaggaca tgacggaagt ctgccccgat gccaccatgc
     1681 tcaactacgt taacccaatg gcgatgaata cctgggcgat gtatgcacgc tatccgcata
     1741 tcaagcaggt agggctgtgc cattcggtgc agggaacggt ggaagagctg gcgcgtgacc
     1801 tcaatatcga tccggctacg ctgcgttacc gttgcgcagg tatcaaccat atggcgtttt
     1861 acctggaact ggagcgtaaa accgcagacg gtagctacgt gaatctctat cctgaattgc
     1921 tggcggctta tgaagcaggg caggcaccga agccaaatat tcatggtaat actcgctgcc
     1981 agaatattgt gcgttacgaa atgttcaaaa agctgggcta cttcgtcaca gaatcgtcag
     2041 aacattttgc tgagtacaca ccgtggttta ttaagccagg tcgtgaggat ttgattgagc
     2101 gttataaagt accgctggat gagtacccga aacgctgcgt cgagcagctg gcgaactggc
     2161 ataaagagct ggaggagtat aaaaacgcct cccggattga tattaaaccg tcacgggaat
     2221 atgccagcac aatcatgaac gctatctgga ctggcgagcc gagtgtgatt tatggcaatg
     2281 tccgtaacga tggcttgatt gataacctgc cacaaggatg ttgcgtggaa gtggcctgtc
     2341 tggttgatgc taatggcatt cagccgacca aagccggtac gctaccttcg catctggccg
     2401 ccctgatgca aaccaacatc aacgtacaga cgctgctgac agaagctatt cttacggaaa
     2461 atcgcgaccg tgtttaccac gccgcgatga tggacccgca tactgccgcc gtgctgggca
     2521 ttgacgaaat atatgctctt gttgacgacc tgattgccgc ccacggcgac tggctgccag
     2581 gctggttgca ccgttaaggg gtaatcgctt ccggcgctct ggtgattaag ccaaagcgcc
     2641 gtagctggcg gcaatgaaag cgggcgagga atatcaccgt cgttaatgcg cccagtagtg
     2701 cgcagaacat atcggattgc gtatcccacg ggtcgccttg agtaccgaga aaatcatctg
     2761 cgccctggcc cattgccagc gctgcccacc actcgattaa ctcatacatc gcgcttatcg
     2821 ccagcgccac gcagcagacc agaaacgcca ccatcttacg tccgcgaacg tacatcccgc
     2881 gtacaaggat ctctcgtgcc accagtgcag gcaccagccc ctggaaaaaa tgccccagtt
     2941 tgtcatacgg attacggctc aaccccagcc attcctgcac ctcaaaacca acggggactt
     3001 tcgcgtaggt gtattgcccg ccgaccatca ggatgatggc gtgaaagaaa atgagcgtat
     3061 agagcagcgg cgttaacggg taacgtttag cggtggcaag cagtagcggc acgacaataa
     3121 tcaccggtgt cacttccatc agccaggtga gtttgtcatg ggccgaaatg ccggtataaa
     3181 tcaggattaa cgttagcgcc agtgcgccgg tgttaagaat taacggcttg agtgtgcggg
     3241 tcatggtgag ttcgcaagtc agggaaaata ctgactattg accagtaagc gtgaaattac
     3301 aatcgcaaaa gaggtggccc agggggatca cctggcagca tgctgccagg cgctgggccg
     3361 aagaggttac ttagtgcagt tcgcgcactg tttgttgacg atctgctgga agaagtcgtt
     3421 acctttgtca tcgaccagga taaacgccgg gaaatcttct acttcgattt tccagatagc
     3481 ttccataccc agctccggat aagcgacgca ttccagatgc ttgatgctct gctgcgccag
     3541 taccgccgcc ggaccgccga tgctaccgag gtagaagccg ccgtgtttat ggcacgcgtc
     3601 ggtaacctgc tggctgcggt tacctttcgc cagcatgatc atgctgccgc cgtgggattg
     3661 cagcagatcc acgtaggagt ccatacgacc tgcggtggtt gggccaagtg aacctgatgg
     3721 ataaccggca ggcgttttcg ccggacccgc gtagtagatc gggtgatctt tgatgtactg
     3781 cggcagttct ttaccggcgt caatcagctc tttcagcttg gcgtgtgcaa tatcgcgacc
     3841 cacgataatg gtgccggtga gcgacaagcg ggtggatacc gggtattgcg aaagctgggc
     3901 aaggatctct ttcatcgggc ggttaaggtc gactttcact gcttcgcctt cacctgcctg
     3961 acgcagttct tctggaatgt actggcctgg attgtgttcc agtttttcga tccagatccc
     4021 ttcgcggttg attttcgctt taatgttacg gtcagcggag caggagacgc ccatgcctac
     4081 cgggcaggat gcgccgtgac gtggcagacg gataacgcgg atgtcgtgcg cgaagtattt
     4141 accgccaaac tgcgcgccaa gaccgagttt ctgggcctct tccagcagtt cctgttccag
     4201 ctggatatcg cggaacgcct gaccatgttc gttcccttcc gtcggcagtt catcgtaata
     4261 gtgagcgctt gctaacttga cggttttcag gttggtttcc gcagacgtac cgccaatcac
     4321 aaacgcgata tggtacggcg ggcaggctgc agtaccgagg gtacgcattt tctcgacgag
     4381 gaagtttttc agtttgccgg gcgtcagcag ggctttggtt tcctggtaga gatacgtttt
     4441 gttggcagag ccgccgcctt tagcgacaca gaggaatttg tactcatcgc catctaccgc
     4501 gtacaggtcg atttgcgcag gcaggttcgt gccggtgttg acctctttat acatgtccag
     4561 cggcgcgttc tgtgaatagc gcaggttatc ttcgatatag gtgttataga cgcctttcga
     4621 cagcgcttct tcatcaccgc caccggtcca cacgcgctga ccttttttac cgacgatgat
     4681 cgcggtgccg gtatcctggc aggtcggcag cacgcctttg gcggcgattt cggagtttct
     4741 taagaattgc agcgccacgt acttgtcgtt ttcgctggcc tctggatcgt gaagaatagc
     4801 cgcaacctgt ttctggtgtg ccgggcggag catgaaagaa gcatcgtgaa aggcttgctg
     4861 cgccagcagg gtcagggctt ctggttccac tttcaggatg gtttcgccgt cgaagtcggc
     4921 aacgctaacg taatcggaag tgagtagata gtattcggta ttgtctttcc ccatcgggaa
     4981 aggtgcctgg tagataaagg gtttgtttga catagcttcc agcctgtaac tctgtatttg
     5041 ttattcgaaa atggcgtgcc gcgtagtcac gggcggcacg caaagtgcat ttataagaac
     5101 ccgtacatcg cggcgaagat ccagccgaag acgcacgata cgctcacacc aatcaacccc
     5161 ggcagaataa agctgtggtt gatgacgaag cgaccgatgt gggtggtgcc ggaacggtca
     5221 aactgaatcg ctgccagatc gctcggataa gtcggcagga tgtaataacc gtagcaagcc
     5281 ggtgctgaag ccacgatgta tgccggatca acgccgatcg ccagcgcgac cggaacaatc
     5341 gccgccagcg ccgcagcctg agagtttaca aacttggaaa ccagcagcag aacaatggca
     5401 taggcccacg gatactcttt caccatttca cccagtacgc cctgaatttc agacatatgc
     5461 gcaccgaaca tggtttcagc catccatgcg ataccgtaca ccgccacgat ggcgatcata
     5521 ccggaacgga agacttcgtt ttttgagata gacgcgggat tggttttggt caggataata
     5581 atcagtgccc cggtcagcag cataaacatc tgaataacca gtaccatcga cagcggtttg
     5641 ccgccgaagg atggacgcag gtccgaatca gcaccaagaa gggcgactac agcgattgcc
     5701 ccgaggaaga tccacattgc cagccagttg cttttcggca gttttttatc cagcagcgtc
     5761 gcggtatcac cgtaaacata ctcacggttt tccggtacgg agatgaattt ttggaactct
     5821 tcgtctttat ccagatcttt accgcggaac cagctgaaga taccgatcgc caggataccg
     5881 attaacgtcg atggaatggt gattgccagc aggtcgagga actcaagatg gcgaccatca
     5941 aaggtgacat tacccagcat cgcaaccaaa gatacgaccg caaccgacac cggactggcg
     6001 ataatcccca tctgtgcacc gatagaactt gccgccatcg gacgttccgg acggatgttg
     6061 ttcttaatag caacgtcgta gatgatcggc agaatggtgt aaaccacatg acccgtaccg
     6121 caaagaatgg tcagcgtaca ggtcacaaac ggcgcgacaa ttgagacata tttcgggttg
     6181 cgacgcagca gcttctcggc aatttgcagc atgacatcaa gaccgcccga agcttgcaag
     6241 gtcgccgatg ccgccaccac cgcaatgata accagcatga catcaaccgg tggtttacct
     6301 ggctgaaggt ggaagacgaa gaccagaatg accagaccga taccgcctaa taaacccagc
     6361 gcgatacccc cctttctggc accataaaac agacatatca gtattatgat aagttggata
     6421 gtaaataaca tgtgtgaacc ctcgcgataa tcctatttaa atttttgctg aatagatcac
     6481 agtcacgttc tgttttgtat gaactgtttt cagagcctga ctaaatatcc gtttggtcgt
     6541 taccggcttg agcaaatacc tcacagtgaa tattggctga ttaaagcagc ggcaaattta
     6601 tgcactgtga taagcggctt tttcagagag aaagactctc tggcgatggg tttatagttt
     6661 gtacagcgca gatgttaatg ttttgtttgc tgttatctta ttgatattta tcggtctgtt
     6721 ttttgttctc agggcaggta tcattaggtt tatagattaa tctgatctac ccatttatgg
     6781 gtaaaaatac acataatgtg ggtgacataa tagttaatta acttttgtta gcgttttgaa
     6841 attaaaaaca ccgttcatct gaagagatat taatttttag cgatgatgga gggataatta
     6901 tatttgatct ggcacaagtt ttactgatga acgatgtaac ttgtgccagg gggattttgc
     6961 attacggtaa taattattac ttacagataa ccagcgatcc gttattggca atattgtttc
     7021 agtagtgagt agtgttctgc ctgaatacgg taacggtaaa ccggacgccc cgtgacgcca
     7081 taatggatac tggtgaacaa tatgtggcag ttgaccagcc agataaggta tttacggcag
     7141 gaaacacgcg agatgttaac ctcgttagcc agctcgtcgg ttgaaaactc atagtcctga
     7201 tgcgcgtcaa tccactggca cagcgtgcgt aacgtctgcg gcgttaagcc ttttggcaag
     7261 cgacgaggat cctgctcgtt ggagctgctg ccgtggatta gctgatcaag ctcggcctgg
     7321 tcataatact gatgtttttc cagcgccatt ttcttttgcc gccagccggt gagcgcctct
     7381 tcaaagcggg aagcctggaa gggtttgatc aggtaatcca caacaccgta atgcagcgaa
     7441 tctttaatgg ttactgcatc ggcagcggag gagatgacaa tcacatcact tttgcaacgc
     7501 gcgttatgca ggacaggcag taaatcgagc ccgttctctt tttgcatata gatatctagc
     7561 aatatcaggt cgataggcga atcgctattg aagataatct ctttggcttt ctccagcgtc
     7621 gaagccgttc cacagcattg aaagcccggg atttgtgcca cgtatcggcg attcagctcc
     7681 gcgaccattg cgtcatcatc gataattaat acattgatca tctgttcgac ctctccccgt
     7741 cccagggtat ctggacaaaa aattgtgtga aaatcccggg ttccgattcc acggcgatgc
     7801 tgccgccgag attttctacc tgttgtttga caagtgctaa accgacgcct cgctcgcttc
     7861 cttttgtcga gacacctttg tcaaaaatgt gatcgatttt gtcgggtgcg atccccggtc
     7921 catcatcgtt aacttcgcag tgcagccagc cgtgacggta gtgcaatgtt acgctaattt
     7981 cgccaccggg ttccggccct aatgcctcca gcgcgttttc tatcagattt cccaacgtgg
     8041 taatcagcgt cgcgacctga tcctcactac cgctgtctgg cagctggctt tcactgttta
     8101 aaatcagcgt atggcctaaa tcggtcgcgc ggttaatctt gctgattaaa aaaccagcga
     8161 aaaccggaga tttgatctta cccagcagag agccaatctc ttcctgatag ttattggctg
     8221 ttttgagaat gtaatcttcc aactgcttat aactcttcag atgcaataat ccgagaatca
     8281 catgcaattt attcataaat tcgtgggatc gttcacgaag tgcgtcagca tagttgacca
     8341 gaccgtcgag tcgctgcatc agtttacgta cttcagtttt gtccctgaag gttgaaatgg
     8401 caccgatgat aacgccatta ctgcgcaccg gaacggtgtt gatcagtaat agtcggtcct
     8461 taatcgtaat ctcttcgtcg cggcgtgggg ttccgtcgcg taacacttcc gagacatcta
     8521 ccacctgtga ccacgagtgg cttagcgtcg acagtttctc atcgtcctgc gacttacggt
     8581 aattcagcaa ttcttgtgcg gcatcgttga tcagcgtgac ctcgccgcga tcgtccacgg
     8641 caacgacgcc ttctttgatg gactgcaaca tggcctggcg ttgctcaaac agtgtggaga
     8701 tttcgtaggg ttccaggccg aaaaggattt ttttcagtac gttaaccaga atgcatgtgc
     8761 cgatcagtcc gaccagcatg ccaaatagta ccgaccagat aatgctccag cgactgtcat
     8821 tgatctgttg ggtcacgcgg cttaactcaa ggccgatcgc caccacgcca atttgtttat
     8881 gattttcatc gtagatgggg gtaaatacgc gtaaagcctg cgccagaaaa ccgcgattga
     8941 tagcgacatt ttcttcgcca ttcagcgctt taaggatgtc atcaccttta aatggctgac
     9001 caatacgctg ggcttcagga tgcgagtagc gaagactgtg catatcggta acgacaataa
     9061 acagcagatc gttgcgtttg cgcacggctt ccgcgatggc ctggattcca ctctcctgcg
     9121 gttttttctg caagccctga cggatttccg gcgagtcggc gagggtacgc gctactgcca
     9181 gagccttgtt agctagcccg tctcgcgtca tatcactgat ttgcgagaag taaatcagat
     9241 gcaccaccaa tagcaccgag aacagtaccg cgctgaccat taagatcaca gtggtactca
     9301 atttcatcgg acgtttgcgt aacatacggt agggcaatga atgtctcatc agcttccttg
     9361 tgtgacaaat ttcttaagca ttatctctga tgaggcgggt aattcaaagg gagtaagaat
     9421 gattggctat ataggggaag agattctggc aacggaaact gccagtgctg tatgaagatt
     9481 ccggggctat gcttatagcg ataatcatac tgatgagaga gggaaggtca tggatcgggc
     9541 gctactggac gggggttatc gctgttatac cggcgaaaag atcgatgtct atttcaatac
     9601 tgcgatatgt cagcattctg gcaattgcgt acgtggcaac ggcaagttat ttaatctcaa
     9661 acgaaagccg tggatcatgc cggatgaagt cgacgttgcc actgtggtta aagtgattga
     9721 tacgtgcccg agcggcgcgc tgaaataccg tcataaataa gcgagggtaa aatggaaata
     9781 cgcgaaggcc acaataaatt ttacattaat gacgaacaag gcaagcaaat cgctgaaatt
     9841 gtctttgtgc cgaccggaga gaatttagcg attatcgaac ataccgatgt cgatgaaagc
     9901 ctgaaagggc aagggattgg taaacagctg gttgcgaaag tcgtggaaaa aatgcgtcgg
     9961 gaaaaacgaa aaattatccc actatgccca tttgcgaaac acgaatttga taaaacacgg
    10021 gagtatgatg atattcgcag ttgatgggag agtacagagt cacgatattt ttcatgctct
    10081 ccgcggtgtg atgcaggaga gcatctgaag ggtaggggga tgcacaaaga atggtcagag
    10141 agagcgtttt tttgtcccaa gtcatcccct ttactgagca aaaaaaaaag aatatctcct
    10201 atatgagaat catcattcgg ggttaataag ttttgcgtcc ccagagcgtt caatattgat
    10261 aggagtcata ttatggaagg taaaaacaag ttcaatactt atgttgtttc ttttgattat
    10321 ccatcatctt attcctcagt gttcttaagg ttaagatcat tgatgtatga tatgaatttc
    10381 tcctctatcg tggctgatga atatgggata ccacgacaac tgaatgaaaa ctccttcgca
    10441 ataacgacat cgttagccgc aagtgaaatc gaagatttaa tcaggctcaa atgcttagac
    10501 ttaccggata ttgattttga cctcagcatt atgacagttg atgactattt ccgtcagttt
    10561 tacaagtagc caggaaggat aagaagaaca taaaaaatgg cactattctc taaaatatta
    10621 attttttatg tgattggtgt gaacatatcc tttgtcatta tctggtttat ctcacatgag
    10681 aaaacacata ttcgtttact tagtgcattt ctggtcggaa taacctggcc aatgagtctg
    10741 cctgtggcat tacttttttc tctcttttag gagctgtagt tgagtggacc cgctggttat
    10801 c
//
LOCUS       DAZFFE010000131        10755 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFFE010000131 DAZFFE010000000
VERSION     DAZFFE010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.131"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..418
                     /gene="yiaM"
                     /locus_tag="ACHJ69_004109"
     CDS             <1..418
                     /gene="yiaM"
                     /locus_tag="ACHJ69_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFO5016981.1"
                     /translation="CIIFINIILRYGFHTSILSIDELSRLLFVWLTFIGAIVAYMDNS
                     HVQVTFLVEKLSPANQQRVSLLTHTLILLLCLGLAWGAIEKTAQDWSNLSPILGVPIG
                     LMYAAAIPTSLIIALLELRHLYRQFTNTPSRNQQGA"
     gene            421..1698
                     /gene="yiaN"
                     /locus_tag="ACHJ69_004110"
     CDS             421..1698
                     /gene="yiaN"
                     /locus_tag="ACHJ69_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFO5016982.1"
                     /translation="MTILIFIVSLLGAIAIGVPIAWALLLCGISLMFWMDIFDVQILA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSQRIVDLPMKLVGHRPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRQANYPVNRAAGLIGSGGIIAAIIPPSIPLIIFG
                     VSSGLSISKLFMAGIAPGIMMGVTLMVTWWWQAKRLNLPCQPKASLREVWQSLVSGIW
                     ALFLPIIIIGGFRSGLFTPTEAGAVAAFYALFVSVVVYREMTFSTLYHVLINAAKTTS
                     VVMFLVASAAVSAWLITIAELPMMVSELLQPLVDSPRLLFIVIMLAIMVISTVMDLTP
                     TVLILTPVLMPLVKEAGIDPVYFGIMFIINCSISLITPPVGNVLNVVCGVAKLKFDDA
                     VKGVAPYVMVLFMLLALFIFIPELITAPLKWMS"
     gene            1711..2697
                     /gene="yiaO"
                     /locus_tag="ACHJ69_004111"
     CDS             1711..2697
                     /gene="yiaO"
                     /locus_tag="ACHJ69_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFO5016983.1"
                     /translation="MKLRSVTYALLIAGVATFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNELLQEKTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFTGLSPVIN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKVSLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FYEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGSYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDTLGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            2701..4197
                     /gene="lyxK"
                     /locus_tag="ACHJ69_004112"
     CDS             2701..4197
                     /gene="lyxK"
                     /locus_tag="ACHJ69_004112"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFO5016984.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCTAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSRGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGLEDEFTLNAVMGTWAVTSGITHGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFAEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPNMAAHQLYQQKYQRYQHLIAALQGYHARIKEHTL"
     gene            4194..4856
                     /gene="ulaD"
                     /locus_tag="ACHJ69_004113"
     CDS             4194..4856
                     /gene="ulaD"
                     /locus_tag="ACHJ69_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO5016985.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QNCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKEIRVKAFIAGRALAGAANPAQVAADFHAQIDSIWGGK
                     RA"
     gene            4849..5709
                     /locus_tag="ACHJ69_004114"
     CDS             4849..5709
                     /locus_tag="ACHJ69_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFO5016986.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMIETGVAIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTQQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     HSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            5703..6398
                     /gene="araD"
                     /locus_tag="ACHJ69_004115"
     CDS             5703..6398
                     /gene="araD"
                     /locus_tag="ACHJ69_004115"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO5016987.1"
                     /translation="MLEQLKADVLAANLALPVHHLVTFTWGNVSAIDETRQWMVIKPS
                     GVEYEVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRFAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRLMTAEEINGEYEYQTGEVIIKTFEERGLNP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(6445..7983)
                     /gene="aldB"
                     /locus_tag="ACHJ69_004116"
     CDS             complement(6445..7983)
                     /gene="aldB"
                     /locus_tag="ACHJ69_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO5016988.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTIEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(8091..9386)
                     /locus_tag="ACHJ69_004117"
     CDS             complement(8091..9386)
                     /locus_tag="ACHJ69_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFO5016989.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMTEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKKINDQQAVYFAHYPL"
     gene            complement(9516..10667)
                     /gene="yiaY"
                     /locus_tag="ACHJ69_004118"
     CDS             complement(9516..10667)
                     /gene="yiaY"
                     /locus_tag="ACHJ69_004118"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO5016990.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         2496 a         2855 c         2812 g         2592 t
ORIGIN      
        1 ttgtattatt tttattaata tcattcttcg atatggattc cacacaagta tattatcgat
       61 tgatgaatta tcccgacttt tatttgtctg gttaaccttt attggcgcaa tcgttgctta
      121 tatggacaac agccatgtgc aggttacttt tttagtcgaa aaactctcac cggctaatca
      181 acaacgtgtt tcgctgctaa cccatacgct cattttattg ctgtgtctgg ggctggcctg
      241 gggcgctata gaaaaaacag cgcaagactg gagcaactta tcgcccattc ttggcgtccc
      301 cattggctta atgtacgccg ccgctatccc caccagtctg attatcgcat tgctggaatt
      361 gcgtcactta taccggcagt tcacaaatac ccccagccga aaccaacaag gagcctgacg
      421 atgacaatcc ttatatttat tgtcagtttg ttaggggcta tcgcaattgg cgttcccatc
      481 gcctgggcat tattactgtg cggcatttca ttgatgttct ggatggatat ctttgatgtg
      541 caaattctgg cacaaacgtt ggtaaatggc gcagacagct tctccctgtt agccatcccc
      601 ttctttgtac tggcgggtga gatcatgaac gcgggcggcc tgtcgcaacg tatcgtcgac
      661 ttgccaatga aactggtagg ccacaggcca ggcggtctgg gttacgtggg cgtgctggca
      721 gcgatgatca tggcaagcct gtccggttct gcggttgctg ataccgcagc tgtcgcggcg
      781 ctgctggtgc cgatgatgcg ccaggccaac tatccggtaa accgcgccgc cgggcttatc
      841 ggttccgggg ggattattgc ggccattatc ccgccttcca taccactcat tatcttcggc
      901 gtctccagcg gactctccat cagcaaactg tttatggcgg gcatcgcacc gggcatcatg
      961 atgggcgtca cgctgatggt tacctggtgg tggcaggcaa aacgactgaa tttaccttgt
     1021 cagccgaaag cctcactgcg agaagtctgg caatccttag tttccggtat ttgggcgctc
     1081 tttttaccga taattattat tggcggtttc cgttcggggt tatttacacc gaccgaagca
     1141 ggcgcggttg ccgccttcta cgctctgttc gtctcggtgg tggtctatcg cgagatgacc
     1201 ttctccacgc tttatcatgt gctgattaac gcggcaaaaa ccacatccgt ggtgatgttc
     1261 ctggtggcct ctgctgcggt atctgcatgg cttattacga tcgccgaatt acccatgatg
     1321 gtatcggagc tattacaacc gttggttgat tcgcctcgct tattattcat cgtcattatg
     1381 ctcgctatta tggtgatcag tacggtcatg gacttaacgc ctaccgtatt aattcttaca
     1441 ccagtattaa tgccattagt gaaagaagcg ggaatagatc cggtctattt cggcatcatg
     1501 tttattatca actgttcaat atcactgatt acgcctccag tcggtaatgt tcttaacgtg
     1561 gtatgcggcg tggcgaaact taaatttgac gatgcggtaa aaggcgttgc gccttacgtc
     1621 atggttttat tcatgttgtt agcattattt atttttatac cggagctcat taccgctcct
     1681 cttaaatgga tgagttaaaa ggaaatcata atgaaattac gttctgtaac ctacgcttta
     1741 ttaattgccg gagtggcgac gttcagcaca tcttctctgg cggcacaatc attacgtttc
     1801 ggttatgaaa cctcacaaac cgattcacaa catattgcgg cgaaaaaatt taatgaatta
     1861 ctgcaggaaa aaaccaaagg cgaactgaaa ttaaaactgt tcccggatag cacccttggt
     1921 aatgcgcagg cgatgatcag cggcgtgcgt ggcggcacta ttgatatgga gatgtctggc
     1981 tctaacaact ttacgggact ctcgcccgtc atcaacctgc tcgatgtccc gttcctgttc
     2041 cgcgataccg ctcacgcgca taaaacgctc gacggcaaag tcggtgatga cctgaaagtc
     2101 tcccttgaag gtaaggggct gaaagtgctg gcttactggg aaaacggctg gcgcgatgtc
     2161 accaactcgc gtgcaccggt caaaacgcct gccgacctga aagggttgaa aatccgcacc
     2221 aacaacagcc cgatgaatat cgccgcattt aaagtttttg gcgctaaccc gatcccgatg
     2281 ccgtttgccg aagtctatac cgggctggaa acccgcacta tcgacgctca ggaacacccg
     2341 atcaacgtcg tctggtcagc aaaattttat gaagtgcaga agtacctttc tctgacccat
     2401 cacgcctatt cgccgcttct ggtggtgatc aacaaagcga agtttgatgg cttaaccccg
     2461 gagttccagc aagcactgat ctcatctgca caggaagcgg gtagctatca gcgcaaactg
     2521 gtcgccgaag atcagcaaaa aatcatcgac ggtatgaaag aagcgggcgt ggaagtcatc
     2581 accgatctcg accgcaaagc ctttagcgat acgctgggta cccaggtccg tgacatgttt
     2641 gttaaagatg ttccgcaggg tgctgatctg ctgaaagccg tggatgaggt gcaataaacc
     2701 atgacgcaat actggctggg gttagattgt ggcggtagct ggctgaaagc cgggctgtat
     2761 gaccgagaag gccgggaggc gggcgtgcag cgcctgccgc tgtgcgcatt aagtccacag
     2821 ccaggctggg cagagcgcga catggcagaa ctgtggcaat gttgcactgc tgtcattcgc
     2881 gccctgctta cccattccgg cgtcagcggg gaacaaattg tcggtatcgg catctccgcg
     2941 cagggaaagg gcttatttct acttgataaa aacgacaaac cgctcggtaa cgccattttg
     3001 tcctcggatc gccgggcgat ggaaatcgtt cgtcgctggc aggaagatgg catcccggaa
     3061 aaactctatc cgctgacccg acaaacctta tggaccgggc atccggtgtc gctgttacgc
     3121 tggctgaaag agcacgaacc agaacgctac gcgcaaattg gctgcgtgat gatgacgcac
     3181 gactacctgc gctggtgttt aaccggcgtc aaaggttgcg aagagagcaa tatttctgag
     3241 tccaatctct acaacatgag tcgcggggaa tatgacccgt gccttaccga ctggctgggg
     3301 attgctgaaa tcaatcacgc cctgccgcct gttgtcggat ctgccgaaat ctgcggggaa
     3361 atcaccgctc agacagccgt actgaccggt ctgaaagcgg gtacccccgt tgtcggcggc
     3421 ctgtttgatg tggtttccac cgcactctgc gccgggctcg aagacgaatt caccctcaat
     3481 gcggtgatgg ggacctgggc ggtgaccagc ggcataaccc acggtttacg tgacggtgaa
     3541 gcgcatccgt atgtctatgg tcgctacgtt aacgatggtc aatttatcgt tcacgaagcc
     3601 agccccacct cttccggcaa tctcgaatgg tttaccgcac agtggggaga aatctcattt
     3661 gctgagatta atcaggccgt tgccagtttg ccgaaagccg gtggcgatct ctttttcctg
     3721 ccgttcctgt acggcagcaa cgccggactc gaaatgacca gcggtttcta cgggatgcag
     3781 gccattcaca cccgtgcaca cctcttacag gctatctatg aaggggtggt gttcagccat
     3841 atgacccatc tcaaccggat gcgcgaacgt tttactgatg tgcacaccct gcgcgtcact
     3901 ggcggcccgg cgcactccga tgtctggatg caaatgctgg cggacgtcag cggtctgcgt
     3961 atcgagctgc cgcaggtgga agaaaccggc tgctttggtg cggcacttgc cgcccgcgtc
     4021 ggtaccgggg tttatcgcga tttcagcgaa gcccaacgtg acttacagca cccggtacgc
     4081 accctgctgc cgaatatggc cgcgcatcag ctttaccaac aaaaatacca acgctatcaa
     4141 catctcattg ccgcacttca gggttatcac gcccgcatca aggagcacac attatgagtc
     4201 gaccacttct gcaactggcc ctcgaccact catcacttga agccgcgcag cgcgacgtga
     4261 cgctgttaaa agacagcgtc gatattgtcg aagcaggcac cattctctgt ttaaacgaag
     4321 ggcttggcgc agtgaaagcc ttgcgcgaac agtgccctga caaaatcatc gttgccgact
     4381 ggaaggtcgc cgacgcgggt gaaacgctcg cgcaacaggc atttggcgca ggcgctaact
     4441 ggatgaccat catctgcgcc gcgccgctcg ccacggtgga aaaaggccac gcgatggcac
     4501 agaactgcgg cggtgaaatt cagatcgagc tattcggcaa ctggacgctg gacgatgccc
     4561 gtgactggca tcgtattggt gtacgacagg cgatttatca tcgtggtcgt gacgcgcagg
     4621 ccagcgggca acagtggggc gaggccgatc tggcacgcat gaaagcgctt tccgatatcg
     4681 gtcttgagct ttccattacc ggcgggatta cccctgctga cctgccgctg tttaaagaga
     4741 tccgcgtgaa agcgtttatt gccggacggg cgctggcagg cgcagcaaat ccggcgcaag
     4801 tggccgctga ttttcatgcg caaatcgact ccatctgggg aggcaagcgt gcgtaatcat
     4861 ccgttaggca tttatgaaaa agcgctggcg aaagatctct cctggccgga gcggctggtg
     4921 ctggcaaaaa gctgtggttt tgattttgtc gaaatgtcgg tggatgaaac cgacgaacgg
     4981 ttgtcacgtc ttgactggag cgccgcacaa cgtgcttcgc tggtcactgc gatgatcgaa
     5041 acgggtgttg ctattccgtc aatgtgcctg tccgcacatc gtcgttttcc ttttggtagc
     5101 cgggacgacg ccgtgcgcca gcgggcgcgg gagattatga gtaaagcgat tcgcctggcg
     5161 cgcgatctcg gcattcgtac tattcaactg gcaggttacg acgtctatta cgaggatcat
     5221 gacgaaggca cccagcaacg atttgctgaa gggctggcgt gggcggttga acaggcagcg
     5281 gcatcacaag tcatgctggc ggtggagatt atggacaccg cgtttatgaa ctctatcagc
     5341 aaatggaaaa agtgggacga aatgcttgcg tcaccgtggt tcaccgttta cccggacgta
     5401 ggcaatctca gcgcctgggg caatgatgtt cccgccgaac tgaaactggg catcgaccgt
     5461 atcgccgcca tccacctgaa agacactcaa cccgttaccg aacatagccc cggacagttc
     5521 cgcgatgtgc cgtttggcga aggctgtgtc gattttgttg gcatcttcaa aacgctgcat
     5581 gagctgaact atcgcggatc atttctgatt gaaatgtgga ccgaaaaagc caaagagccg
     5641 gtgctggaga ttattcaggc gcggcgctgg attgaagcgc gtatgcagga ggccggattt
     5701 atatgttaga gcaactgaaa gctgacgtgc tggcggcaaa cctggcgctt cccgttcatc
     5761 atctggtgac gttcacctgg ggcaatgtca gcgcgataga cgaaacgcgg caatggatgg
     5821 tgattaagcc ttccggcgtt gagtatgagg taatgaccgc cgacgatatg gtggtggttg
     5881 agatagccag cggcaaggtg gtggaaggca gcaaaaagcc ctcttccgac acgccaacgc
     5941 atctggcgct ttaccgtcgc tttgcggaga ttggcggcat agtgcatacc cactcgcgcc
     6001 atgccaccat ctggtcgcag gccggacttg atctccccgc ctggggcact actcacgccg
     6061 attattttta cggagccatc ccctgcacgc gcctgatgac cgcagaagag atcaacggcg
     6121 agtatgaata ccagaccggc gaagtgatca ttaaaacctt cgaagagcgt gggttgaacc
     6181 cggcacaaat ccctgcggtg ctggtgcatt ctcacggccc gttcgcctgg ggaaaaaatg
     6241 ccgccgatgc ggtgcataac gccgtggtac tggaagagtg cgcctatatg ggtctgttct
     6301 cgcgccagct tgcgccacag ctccctgcga tgcaaaacga actgctggat aagcactacc
     6361 tgcgtaagca tggggccaat gcctattacg ggcagtagcc atacctgata gtgcccggca
     6421 atacacgccg ggcagccttt cacatcagaa cagccccaac ggtttatccg agtagctcac
     6481 cagcaggcac ttggtttgct ggtaatgctc aagcatcatt ttgtgggttt cgcggccaat
     6541 gcctgattgt ttgtagccgc caaacgccgc atgtgccggg taagcgtgat aacagttggt
     6601 ccacacgcgc ccagcctgaa tgccgcgccc catcttatag gccagattac cgttgcggct
     6661 ccagacgccc gcgcccaggc catattgcgt atcgttcgcc agctccagcg cttcttctat
     6721 cgttttgaag gtggttaccg ccagcaccgg gccaaaaatc tcctcctgga acacgcgcat
     6781 attgttctga ccaaacaaaa tcgtcggttc gaggtagtag ccgtctttca gttcgccttc
     6841 cagcagcttg cgccgcccgc cggtgagcac atcagcgccc tcttttttac cgatatcaat
     6901 gtagttgagg atggtttcca gttgcccgtg agaaacctgc gcgcccattt gcgtcacgct
     6961 gtcgagcggg ttaccgctgc gaatgctttc gacacggcgg atggcgcgtt ccataaagcg
     7021 ttcgtagata gactcctgca ctaaagcacg gcttggacag gtgcaaactt cgccctggtt
     7081 aaaggcaaac agtgcaaagc cttccagcgc tttatcgaaa aaggcatctt cttcatccat
     7141 cacatcagca aagaagatat ttggcgattt gccgcccagc tccagcgtca ccggaataat
     7201 gttttgcgtg gcgtattgca taatttgttg gcccacttcc gttgagccgg taaacgccac
     7261 tttggcgatg cgtttcgagg tcgccagata ttcgccaatt tccccacctg cgccgttgac
     7321 cacgttcacc acccctggcg gcagtaaatc accgacgatt tccattagca gcagtacaga
     7381 aagcggagta agacgtgcgg gtttcagcac cacacagttg ccagccgcca gcgcgggggc
     7441 cattttccag ctcgccatca gcagcgggaa gttccacgga ataatctgcc ccaccacgcc
     7501 taacggttcg tggaagtgat aagccacagt ttcgctatcc acttcactaa taccgccttc
     7561 ctgcgcccga atacacgagg caaaatagcg aaaatggtca atcgccagcg gcacatcagc
     7621 agcactggtt tctcgaatgg gtttgccgtt atcccaggtt tcagcggtcg ctaacagttc
     7681 gaggttctgc tccatccgat cggcaatctt aaacagaatc gccgcgcgat cctgcaccga
     7741 agtatgcgcc catttatctt tcactttgtg cgcagcatcc agcgccagat cgatgtctcg
     7801 tttgcccgaa gacgccactt cgcacagcag ctgcccggtc accggagtca ggttctggta
     7861 atactcgccg tcggcagggg ctacccactc gccgccgata aagttgtcat aacgggcttt
     7921 taacttaaga ggaaaaccat actcgccggg cttaatctgt gctgaagggg gattattggt
     7981 catgatcgtc tccttgcggt gtgaggtata acaagggtag acgtgactgg cgaaatcttc
     8041 gccagtcgat aacagcttta cgacggctat cacgaattta cgggcaaggg ttacagcgga
     8101 taatgcgcga agtacacggc ttgctggtca ttaattttct tctcttgcgc aaacccttct
     8161 ttaacaattg tccgtaaatc agctcgagcc gtattatcag agacgcccag gctcgcgcta
     8221 atttcagcaa ctgtgtactc tttccccgga ttggcgagca tcacattcag gagcgttacc
     8281 tggcgcggat tcaggcgttt aagcacacca gagctaaaca gcacactatc gagttttgcg
     8341 cttcgttgtg taatttcatc gatatgctgc tgccagtttt gcagagcacg tttaatgaca
     8401 cccgcctgat attcaaggaa ataagtcaga tccataccat ctgattcagt gtactggtag
     8461 ctggcggcat atttgaccgg tgcagcatga agcaaacggc tgatggaaat atatttaaag
     8521 acatcgtagc ccgatttcag catataccag taaaacagcg cccgactggt gcgcccgttg
     8581 ccatcccgaa acggatgttc atgggccagc ataaaatgca gaatgcaggc acggaccaac
     8641 ggatgaatat acccttcatg gctgtttaac cactggcaaa ctttttccag ccgttccggt
     8701 aacaatgccg cagcgggcgg ttgatgcact atattgccat catagtcggc gatcacaaca
     8761 tcatctgttt cacgaaattc tcccggacga tatttcgcgt cattgattcc acccatacca
     8821 atggcatgta actggcgaat aagcgcaggt gtcagcggtt ctgcgagtaa gtgcggaatt
     8881 tccgtcatca gccgggcatt tcccgcaatc atatgctcgt cttcagtgcg tggcgaccgc
     8941 cctgtttcca gcatttttct cgccaccagc gttgtagtat tcgcaccttc cagctgggag
     9001 ctggtgatcg gctcttccag gcgtaactgg cttaactcgg ctccggcccc gcgtaaccgt
     9061 tcagtctgat ctttcagcgc cagcgcggta gcacgtttgt cgacaagctc gcacgcttct
     9121 gcaatcaccg gggtaatgtt gaatccggcc tgttcacccg cttcattgca ataattaatt
     9181 cgttggatcg ccgcgttgcg cgccagacgg gtaagcgtcc acgccacatc gacgttttct
     9241 ccttttttga tgcggtactg caactcatca aaaggcagat aacgtccttt ttcatcggta
     9301 acccggttaa agcgcaggtg ggccaggagt tcctcacccg ataagtgtga taaaggaata
     9361 agtccgggag gtgatttgat agccatgtgc ttatctcaaa aaagcgcaat attgagatag
     9421 tgtatagcag gaaagagaaa ctatctcaaa aaaagcaagt tttgagatag ttgaaaaagc
     9481 ggctgacaac tcgccagccg ttgtggaaat gatgattaca tcgctgcgcg ataaatcgcc
     9541 acaatttctt cgtgagttgc ctggatcggg ttagtaaagc cgcaggcatc tttcagggca
     9601 ttagtcgcca gaaccgcgaa atcttcttct ttcacgttca ggtcgcgtag gcctgccggg
     9661 atatccactt tcttcgccag ttcacggatg gcgttaatgc aggcttcagc accttctgcg
     9721 tcgtttttac ctgtcacgtt cacgcccatt gcagcggcac aatcacgcag acgtgcagcg
     9781 gcgactttgc tgttgaatac ctgaacatgc ggcagcaaaa cggcgttaca tacaccgtgt
     9841 ggcaggttgt agaaaccgcc cagttggtgc gccatcgcat gaacataacc cagagaagca
     9901 ttattgaacg ccataccggc gaggaactgg gcataagcca ttgcttcacg cgctttagca
     9961 ttgctgccat cttcaacggc taacggcagg ttttcggcaa tcatggtcac ggctttcagt
    10021 gcacaagcgt cagtgatcgg cgtggcggca atagaaacat acgcttcgat agcgtgcgtt
    10081 aaggcatcca tacccgttgc agcggtcagt gacttcggca taccgatcat cagagaggag
    10141 tcattgacag aaagcagcgg agttacatgc ttatccacaa tcgccatttt gatgtgacgc
    10201 gcttcgtcag tgatgatgca gaaacgggtc atttcagagg ccgtacccgc cgtggtgttg
    10261 atggcgatca tcggcagctg cggttttgca gagcggtcaa cgccttcata atcgcggatg
    10321 tcaccgccgt tggctgcaac cagcgcaata ccttttgcgc agtcgtgtgg agaaccaccg
    10381 cctaaagaaa tcacgctatc gcaattattt tctttaagta atttcaaacc tgcagcgacg
    10441 ttttccgtgg tggggttagg ttgggtgcca tcataaataa cgctaaaaat attgcgttct
    10501 tccagtgctt tttgcacatc acccgccata cccaatttcg ttaacatgtt gtcggtgaca
    10561 attaaggtac gggtaaatcc ataatctgcc atcatattca ttgcatcatt caatgaatca
    10621 gcaccaataa cattcacgga aggaataaaa aaggttgaag ctgccataac acactcctga
    10681 aagtgtaaaa aagggcgggc agcttactga ggattttcat ttgaaaatat gatcaacatt
    10741 accgtttatt aaaat
//
LOCUS       DAZFFE010000132        10739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFFE010000132 DAZFFE010000000
VERSION     DAZFFE010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.132"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            61..681
                     /locus_tag="ACHJ69_004119"
     CDS             61..681
                     /locus_tag="ACHJ69_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5016991.1"
                     /translation="MVLITTTKNIFIIQAIKSMRWRGFSYVKVIAVDDYSSLIRDFGT
                     SCFRDEQSLLLPVFPDNDSTTCIRSLYFIHQWLKSQPVNIPCLAWGDNGVVSNKGIPF
                     IPWKLTAEQFCFYIDKFINGWRCKKNIRANHKVSFLERQLSPREKSIFIYTKEGKDIA
                     WISNTLGISSKTVWTHRRRAMDKLGVKRLHQFINIPRSLISENIQL"
     gene            706..8643
                     /locus_tag="ACHJ69_004120"
     CDS             706..8643
                     /locus_tag="ACHJ69_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phase-variable autotransporter adhesin UpaE"
                     /protein_id="HFO5016992.1"
                     /translation="MNKIYKVIWNHTTQKWDVVSELTSCRKKCKSTRLGIALSAMVLG
                     GAIAINCNNAMADVILSPDWRPGTNNSGVGAATVSGKTEYITGPNVVQSGGSGLIWMT
                     VEQAILNGYTTGDNLSGLIYVNTGEKTKTITVKDEVTGASQTLQVFDTDSFSQRDAGT
                     GGNETIPGFSGTADFFNATRFVTANNGGTAILDVGSPAIGNFFKNTQLAVADGEGSSV
                     VWNSVNDFYFQPGATMQGGGVTQKIIDSMKYAGTITDWAGKVHHINSLDDLKQYNQYL
                     IKSLEDKTLSYKQYDAEFNKALIVTKHNYNVDMTAGGRIDSTPYKENVGLLAVLHATN
                     NARAILGKTGKLTGVLPAYGNGGGIVATNGGTGVNEGVIDAIGTEMIAYQDSTIVNDG
                     TLFVWDNNDKYALQAEGMVAGSNGSSAINNGVINIRPFKNAFAPEGINTAIVVSNGGM
                     ATNKGTINITADASTNDNNGKTRGVNVGAGGSFINSAFGSINVGIAEDKTATHSAVGS
                     VAIEVQNGANKVVNEGTIFLGRGAQGNYGILAKDAGTVDVVNKGTITIDGHDSDAPAL
                     NVGMLANNSSGMKNSGIINVNGLNSTGLQVINAGQLNSDGTINVGGKGISSGFRNYGA
                     WVEGAGSNVNVSGKISLAGTGAVGVFAKDGGSLTLSGNGAVLFGSSDQIGFYVYGKDS
                     AIHNTGSGVMDVSTENSTLFRIASGATFQGTADASSALTASGKNSYALIATGKSDGGV
                     ASTVTSGGMTINLTGEGATATLIEGGAQGTIESNAIINMDNASAIAGIADGNGYDISG
                     KLINPKDKTTLLTAGAQLSSTQDKVTGYIARNGATLNNTGNIIFTGKNTVGVRVEEGA
                     VGTNSGNITVQDGGVGLIANATQDVTTINNSGNLVLKGGDNANRTTGIKASGTTTTVN
                     MTAGTISLQGQGAIGVEASNKGTVNLDGSAVPNFAADGSGITDQIAFRIIGDGATIKT
                     NIAPGTLLDASGERSVLFRIEDGAKQAGSLLMKTSGTGSRGIWATGKGSNVLADAGSD
                     FQILGAQAQGLYVTGGATATLKQGASVNLVGDGAVVAEVDGNEYALDGSITQTNTGSV
                     ITNEADISSPLNNARGFITRNQGLLINNGNIDFTAGTDNIGVWVDNGRFENTGSRIAV
                     NGVALFVEGAQSQITSTGGDIVAVDGEAAIKLGAGASLNLAGSGLGTIEGQKNAHGIL
                     LDTGAVGLVIDGAKINVNAAGAVGHGIENRAEIEGIQLTNTTEINVADGIGVRTSASL
                     AKTNSGTINVDGSGIALAFQKADGSETDNNLDMSDSAGLVINLKGTDGTGIFANTKDG
                     AVVKSGASVNVTQADGGSALVVNNAASEVVQSGNLISASLSHAVVDASKAQSFTNKGQ
                     IKAASTTGTAMAFDDAVNTTVLNDSGAEIQGVVVLNGGDNTFTNKGSITGTVSAKEGN
                     NTFLFDDGSTLTGEVTAGNGNNNVTLNGKAHVDQVTAGTGKNTFTIKGEGATWNLLDG
                     GQGDSDSLIFDNAIHTLDSVVKLQNFEHVGLKNSSLVTLKEALVLTDGGNGPGSVDIE
                     SGSELAIIPAVAGNFTFDPLLTGKGTLSARLDADTSAFEFSHNVGDQFAGTLKLGTSS
                     FALEGLNTSGLTHAMLMSETGNITTVGSGVQQIGGLGFNGGTLIFGSVMPGDTIASNS
                     IETSAAGTLDIRGKGTIQVTMPDEVINDIPAVDTRKNLLEQDDAQTLVTLVNAAGTVT
                     GTGGQLQLVDENGQAISHSQTFDVTQGGEVVAQGNYDYKLLGSSDGIKGDGLYIGYGL
                     KSLDLQGTGDKALVLTPRANAQGLQTDLGAQLTGAGDLAIEAAGQVVTLSNGGNNYTG
                     DTLVRSGTLQMANDNVLGATGNLNVASNGVFRTNGYSQTVGALQTETGAHIQLDSGSV
                     LTVSGTQRQPGDDNGGIIENNVLSGEGTLAVTGSNLTVHGTNIGFTGNASLTQGALVE
                     MNGAQGLGSQGSISFESLNDRLAIDIADGSGVSSNLSKSLSGEGSVGILNTTDLTLSG
                     DNSNFSGEFRVQKDAALRASDEKHLGTGLIDSDGVTWLTASGNWLLKNDITGSGALVK
                     QGAGNLIINHELTYTGDTTVESGVLIVGDDSVTRAAGATLSGSKNIHVLNGGILSGLG
                     TVSGQVNNQGTLASLNALSGYETAEVGNFTVGSLTNTGVIRLAGGKTGNTLTVNGDYT
                     GGGTLIINTVLGDDTSATDKLIVTGNTSGDTGVVVNNVRGQGAQTADGIEIVHVGGQS
                     DGNFRLQNRAVAGAWEYFLHKGNAGGTDGNWYLRSELPPEPQPQPQPQPQPQPQPQPQ
                     PQPHPTPDKPVQKVYRPEAGSYIANIAAANTLFNIRMHDREGETYYTDVFTGEKKATS
                     MWMRHIGGHNRWKDSSSQLNTQSNRYVVQLGGSIAQWTDGQDRLQLGIMAGYGNEKSS
                     TTSSLSGYKSKGAINGYSTGLYGTWQQNDGNDNGAYVDTWIQYGWFNNTVNGEKLAAE
                     SWKSRGFTGSVEAGYTFKAGEFTGSQGSHYDWYIQPQSQITWMNVRASEHTEKNGTKV
                     QLSGDGNIQSRLGVRTYLKGKSASDDNKAHQFEPFVEVNWIHNTRSWGVKMDNTALSQ
                     DGATNIAEVKTGVQGKLSDNLNVWGNVGVQAGDKGYSDAQAMLGIKYIF"
     gene            8691..9221
                     /locus_tag="ACHJ69_004121"
     CDS             8691..9221
                     /locus_tag="ACHJ69_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HFO5016993.1"
                     /translation="MSSVMKFSVVNAVVLLLLIVLTGVFYMKKSKEIAVVNIDAIYNQ
                     SAAGDMVRKHLNEVKSILEKGYEDAQKAFADNANKEALLLDARQKLERQYVIEENSAK
                     MVLSRAIDNAIGTWRKDNPKNIAVLSSGVVLAHNESADITKEVMALVEKENLVFNQLP
                     KVEVHNKNEEKPEQSK"
     gene            complement(9659..9733)
                     /locus_tag="ACHJ69_004122"
     tRNA            complement(9659..9733)
                     /locus_tag="ACHJ69_004122"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9698..9700),aa:Arg,seq:cct)
     gene            9929..>10739
                     /locus_tag="ACHJ69_004123"
     CDS             9929..>10739
                     /locus_tag="ACHJ69_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5016994.1"
                     /translation="MALNDTKLRGLYGKPYSGPAEITDGDGLSVRITPAGTITFQYRY
                     RWNGKPVRLTVGRYPSTSLKDARVIVGEMRALYMKGVNPKNYFAPSDGELTLKECLDQ
                     WWDKYVTDLKPNTQTLYRSVVYNTMYTQFEGSPVASIPVSAWARFFDKQESLNKKKAR
                     VLLLQLRSVINWCISRQLIPSCELLKLSVKNIGKKPDVGSRVLTYTELAKIWLALENS
                     KVFTSNKVLHQLLLLWGARLSELRLATASEFNMEDLVWTTPKEHSKMGNIIR"
BASE COUNT         2967 a         2127 c         2897 g         2748 t
ORIGIN      
        1 ccccccccgg tgtgcaaagc aaaaggaaat tgggaaaggg gataacagga aattgattgc
       61 atggttttga ttacgacgac aaaaaatatt tttataattc aagcaattaa gagtatgcgt
      121 tggcggggtt tttcgtacgt gaaagtcatc gctgtggacg actattcttc tctgattcgt
      181 gattttggca catcttgctt tcgggatgag caatcgttgt tgttacctgt attccctgat
      241 aatgattcta ctacctgtat acgctcactg tatttcattc accaatggct aaaaagccag
      301 cctgtcaata ttccgtgcct ggcatggggg gataatggtg tagttagtaa taaaggcata
      361 cctttcatac cgtggaaatt aacagcagag caattttgtt tctatataga taaatttatc
      421 aatggctgga ggtgcaaaaa aaatattcgc gcgaatcata aagtttcttt tttagaacga
      481 caattatccc cgagggagaa aagtatcttt atatatacca aggaaggaaa agatattgca
      541 tggatctcga acaccttggg tatatccagt aagactgtgt ggacgcatcg tcgtagagct
      601 atggataaac tcggggtaaa acgtttacat caatttataa atattccgcg ttcccttatt
      661 agtgaaaata ttcaattata atgtacgttc aagagtatta gtgttatgaa taaaatatat
      721 aaagtcattt ggaaccatac aacccaaaaa tgggatgttg tttcagaatt gacgagttgt
      781 agaaaaaaat gtaaatcaac tcgcttagga attgctttat cagcaatggt tctggggggc
      841 gctattgcaa ttaattgcaa taacgcaatg gcagatgtca tattgtcacc agactggcgt
      901 ccgggtacga ataactcggg tgttggggct gcaacagtaa gtggtaaaac cgaatacatc
      961 actggtccaa atgtcgtcca gtctggtggg tcaggtctta tctggatgac tgtagaacag
     1021 gcaattttaa atggctacac cactggagat aatttatccg gattgattta cgtcaatacc
     1081 ggagaaaaaa caaaaaccat tacagtgaag gatgaggtta ctggcgcttc tcaaacactt
     1141 caagtgtttg atactgactc tttctcacaa cgcgatgcgg ggactggggg aaatgaaacc
     1201 attcctggtt ttagtggcac tgcggatttt ttcaatgcga cacgttttgt aacagccaat
     1261 aatggcggta cagctatttt ggatgtgggt tcaccagcaa tcggtaattt ttttaaaaat
     1321 acacagcttg ctgtagctga tggagaaggt tcctctgttg tatggaactc ggtcaatgat
     1381 ttttattttc agcctggtgc aaccatgcag gggggcggcg ttactcaaaa aatcattgac
     1441 tcaatgaaat atgctggaac aattactgat tgggcgggaa aagtacacca tattaactct
     1501 cttgatgatt taaagcaata taatcaatat ttgataaagt cactagagga caaaacgctt
     1561 tcttataagc agtatgatgc tgaatttaat aaggccctta ttgtcaccaa gcataattac
     1621 aatgtggata tgaccgctgg gggacgtata gactcaactc cttacaaaga aaatgtaggg
     1681 ctgcttgctg ttctccatgc aaccaataac gcacgagcaa tattaggtaa aacgggtaaa
     1741 ttaactggag ttcttcctgc ctatgggaat ggaggaggga tcgttgcaac taatggtggg
     1801 accggtgtta atgaaggggt cattgatgcc attggtactg aaatgattgc ctatcaagac
     1861 agtaccattg ttaacgatgg tacacttttt gtttgggata ataatgataa atatgctctc
     1921 caggcagagg ggatggttgc cggtagtaat ggttcttcag ccattaataa tggtgttatt
     1981 aatattcgcc catttaaaaa tgctttcgct ccagagggga ttaacaccgc aattgttgtt
     2041 agtaatgggg gcatggcaac aaataaaggc acaataaata ttactgccga tgcatcaact
     2101 aatgataata atggcaaaac acgaggtgta aatgttggag ctggaggctc ttttattaac
     2161 tcggccttcg ggagcatcaa tgtaggtatt gcggaggata aaactgcgac tcattcggct
     2221 gtgggttctg ttgcgattga agtacaaaat ggtgcaaaca aagtcgttaa tgaaggtact
     2281 atttttttgg gcaggggggc tcaggggaac tacggaatcc tggcaaagga tgccgggact
     2341 gttgatgtgg taaataaagg gactatcact attgacggtc atgacagtga tgcacctgca
     2401 ctgaatgttg gcatgctggc aaataatagc tccgggatga aaaactccgg gattatcaat
     2461 gttaatggtc tgaacagcac cgggctacag gtaatcaatg caggacagtt gaattctgac
     2521 ggtacgataa atgttggcgg caagggtatc agtagtggtt tccgtaacta tggtgcctgg
     2581 gtggaaggtg ccggaagcaa tgttaatgta tccggaaaaa tcagtcttgc cggtacgggg
     2641 gctgtggggg tttttgctaa agatggcggc agtctgaccc tgtcaggcaa tggtgccgtg
     2701 ctatttggca gcagcgatca gataggcttt tatgtctatg gaaaggactc tgccattcat
     2761 aataccggaa gcggtgttat ggatgtgtcc actgaaaact caacattatt ccgtattgcc
     2821 agcggtgcga cattccaggg aactgcagat gcttcttctg cacttacggc gtctggtaag
     2881 aactcttatg cacttattgc cacggggaaa tcggatggcg gtgtggcctc gacagtaacg
     2941 tctggaggaa tgaccatcaa cctgacgggt gagggggcta cagcgacttt aattgaaggg
     3001 ggagcgcagg gcacaattga aagtaatgcc attatcaata tggataatgc cagtgcgata
     3061 gccggtattg cggatggcaa tggctatgat atttccggca aactcattaa tccgaaggac
     3121 aagaccacac tattaacggc gggggctcag ttaagttcca cccaggataa agtgaccggg
     3181 tatatcgccc gtaatggggc cactctaaat aataccggta atatcatctt tactggaaag
     3241 aatacagtgg gcgtccgggt tgaggagggg gctgttggta ccaacagcgg aaatattaca
     3301 gttcaggatg gtggtgtggg actaattgct aatgccacac aagatgttac aacgattaat
     3361 aactcgggaa atctcgttct caagggagga gataatgcta accgtacaac gggtataaaa
     3421 gcatctggta caacaacaac ggttaatatg accgcgggta ctatatcttt gcagggacag
     3481 ggggcgattg gtgttgaggc cagcaataaa ggaactgtta accttgatgg ttcggcagta
     3541 ccgaactttg ctgctgacgg ctctggtatt accgatcaga ttgcttttcg tattatcgga
     3601 gatggtgcaa ccattaagac gaatattgca ccgggaactc tgctggatgc cagtggagaa
     3661 cgttctgtac ttttccgtat tgaagatggg gcaaaacagg ccggctctct gctgatgaaa
     3721 acctctggga caggcagccg tggtatctgg gccacaggga aagggagcaa tgtcctggct
     3781 gatgctggca gtgatttcca gatcctgggc gctcaggctc agggattata tgtaactggt
     3841 ggtgcgacag cgacgctgaa acagggggca tcagttaacc ttgtagggga tggcgctgtt
     3901 gtcgcggaag ttgacggaaa tgaatacgct ctggatggca gtattacaca aacgaatact
     3961 ggctcggtca ttaccaatga ggcagatatc tcttcgccgc tgaataatgc caggggcttt
     4021 attacgcgta atcagggact gttgattaac aacggcaaca ttgatttcac tgccggtacc
     4081 gataatatcg gcgtctgggt tgataacggc cgctttgaaa atacaggaag ccgtattgcg
     4141 gtcaatggcg ttgcattatt tgttgaaggt gcacagtctc agattaccag cacaggaggg
     4201 gatatcgtcg ctgtggatgg tgaggctgcc attaagctgg gggcgggcgc gtcactgaac
     4261 ctggcaggga gtggcttggg tacgatcgaa ggtcagaaaa atgcgcatgg catcctgctg
     4321 gatacagggg ctgtggggct ggttattgat ggtgcgaaaa tcaatgttaa tgctgcaggt
     4381 gcggtcggtc acgggattga gaaccgcgca gaaattgaag gcattcagtt aaccaatacg
     4441 actgaaatta atgtggctga tggcattggt gtacgtactt ctgcctccct ggccaagacc
     4501 aacagcggca ctattaatgt agacggcagt ggaattgcac tggcgttcca gaaagctgac
     4561 ggaagtgaaa ccgataataa cctggatatg tctgactccg ctggattggt cattaacctg
     4621 aagggtacgg acggcacggg tattttcgcc aacactaaag atggtgctgt cgtgaaaagc
     4681 ggtgcaagtg tcaatgttac acaggccgat ggcggttccg ctctggtggt taacaatgca
     4741 gccagtgaag tggttcagag cggtaatctc atctctgctt ctctgagtca tgccgtagtg
     4801 gatgcttcaa aagcacaatc ctttaccaat aaaggtcaga ttaaagctgc gtccaccacc
     4861 gggactgcaa tggcgtttga tgacgccgtg aataccaccg tactgaatga cagcggtgct
     4921 gaaattcagg gggttgtggt tctgaacggc ggtgataaca cattcaccaa taaaggcagt
     4981 attaccggaa ccgtcagtgc gaaagagggt aacaatacat ttttatttga tgatggcagc
     5041 acactgacag gagaagtgac tgcaggaaat ggcaataata atgtaacact caatggtaag
     5101 gctcatgttg atcaggttac tgccggtacc gggaagaaca ccttcaccat taaaggcgaa
     5161 ggggcaacct ggaacctgct ggatggcggg cagggagatt ctgattccct gatttttgat
     5221 aacgccattc atacgctgga ttctgttgta aaactacaga attttgaaca tgtcgggctg
     5281 aagaacagtt cacttgtcac tctgaaggaa gctcttgtgc tgaccgatgg ggggaacggt
     5341 ccgggttccg tcgatattga atcgggcagc gaactggcca ttattcccgc agttgcaggc
     5401 aactttacct ttgatccact gttaacaggc aaaggaacac tgtctgcccg tcttgatgcc
     5461 gacacatctg cttttgaatt cagccataac gtcggggatc aatttgccgg aactctgaag
     5521 ctgggtacca gtagctttgc tctggaaggg ctgaatacga gcgggttaac ccatgcaatg
     5581 ctgatgtctg aaaccgggaa tatcacaacg gttggctccg gtgttcagca gattggcggt
     5641 cttgggttca atggcggaac gctgattttt ggttccgtta tgccgggcga taccattgcc
     5701 agcaacagta ttgaaacctc tgctgcaggt acgctggata tccgggggaa aggcacaatt
     5761 caggtcacca tgccagatga agtgattaat gatattccgg ctgttgatac ccgtaagaat
     5821 ttgctggagc aggatgatgc gcagaccctg gtcacgctgg tgaatgcagc gggtaccgtc
     5881 accggtactg gcgggcaact gcaactggtg gatgaaaacg ggcaggctat ttctcacagt
     5941 cagacgtttg atgtcactca gggcggtgaa gttgtagctc agggaaatta tgactataag
     6001 ctgctgggaa gctccgacgg tattaaaggt gacggactgt acataggcta tgggctgaag
     6061 tcgctggatt tacagggaac cggtgataaa gcgctggtgc tgacaccgag agcgaatgcc
     6121 cagggactgc agacagatct tggcgcacag ttaacggggg caggggatct ggccatcgaa
     6181 gctgcggggc aggttgtcac actgtctaac ggcggtaata actacaccgg ggatacgctg
     6241 gtgcgcagcg gcacattaca gatggcaaat gataatgtac ttggcgcaac aggtaatctg
     6301 aacgtcgcca gcaatggcgt cttcagaaca aacggataca gccagactgt cggtgcactg
     6361 cagacagaaa cgggagcgca cattcagctt gatagcggta gtgtactaac ggtcagcggc
     6421 acgcagcgtc agccggggga tgataatggt ggcatcattg aaaacaacgt gctgtccggt
     6481 gagggaacgc tggctgtcac gggcagcaat ctgacggtac atggcaccaa tatcgggttt
     6541 accggtaatg ccagccttac ccagggggcg ctggttgaaa tgaacggcgc acaggggctg
     6601 ggaagccagg gctccatcag ctttgaaagc cttaatgatc gcctggcgat tgatatcgcc
     6661 gacggtagcg gagtcagcag taacctgagt aaatcgcttt ccggagaagg ttcagtgggt
     6721 atactgaaca ccactgatct gacgctttcc ggggataaca gtaatttcag cggtgaattc
     6781 cgtgtgcaga aagatgctgc tctgcgggct tcagatgaaa aacacctggg caccgggctg
     6841 attgacagtg atggtgtcac ctggcttacc gccagtggaa actggttact gaagaatgat
     6901 attactggct ccggcgctct ggtgaaacag ggggcgggta atctgatcat taatcatgag
     6961 ctgacttata ccggtgatac cacggttgag agtggcgttc tgattgtcgg tgatgattca
     7021 gtcacccgtg ccgctggcgc gacgttgtca ggatccaaaa acattcatgt tcttaatggc
     7081 ggcatactga gtgggctggg gaccgtttcg ggtcaggtta ataaccaggg aacactggca
     7141 tcactgaatg ctctctcagg ttatgaaacg gcagaggtgg gcaattttac cgttggctca
     7201 ctgaccaata ccggcgtcat tcgtctggcc ggtggtaaga caggaaatac cctgaccgta
     7261 aatggcgatt acaccggtgg cggtacgtta attatcaata ccgtgctggg ggatgatact
     7321 tctgccactg acaaactgat tgtcacgggt aacacctccg gtgataccgg cgtggtggtg
     7381 aataatgtcc ggggccaggg ggctcagacc gctgacggca ttgagattgt gcatgtcggt
     7441 gggcagtctg acggtaactt cagactgcag aaccgtgcgg tggcaggggc gtgggaatat
     7501 ttcctgcata aaggtaatgc gggtggtact gacggtaact ggtatctgcg ttctgaattg
     7561 ccgccagaac cacaaccgca gccgcagcca caaccgcagc cgcagccgca gccacaaccg
     7621 cagccacagc cacaccccac gccggataaa cctgtacaga aggtctatcg tccggaagcg
     7681 ggctcttata ttgcgaacat agcggcagcc aacacgctgt tcaacatcag aatgcacgat
     7741 cgtgaggggg aaacttacta taccgatgtg tttactggtg agaagaaagc aacaagtatg
     7801 tggatgcgtc atatcggcgg tcataatcgc tggaaggaca gcagttcaca gctgaatacg
     7861 caaagtaacc gttatgttgt gcaactgggt ggcagcattg cgcaatggac tgacggtcag
     7921 gaccgtctgc aactggggat tatggccggt tatggaaatg agaagagttc cacgacatcc
     7981 tcgctttcgg gatataaatc taaaggtgcg atcaatggat acagcaccgg tttatacgga
     8041 acgtggcagc agaatgacgg taatgacaac ggggcttatg tcgatacctg gatccagtac
     8101 ggatggttta ataataccgt taatggtgaa aaactggctg cggagtcctg gaaatcccgt
     8161 ggatttaccg gttcagtgga agcgggttat acctttaaag ccggcgaatt taccggtagc
     8221 cagggtagtc actatgactg gtatattcag ccgcagtccc agatcacgtg gatgaatgta
     8281 agagccagtg agcatacgga gaagaatggc acgaaagttc agctcagcgg agacggtaat
     8341 atccagagtc gtctgggcgt cagaacttac ctgaaaggta agagtgcgtc tgatgataat
     8401 aaggcgcatc agtttgagcc atttgtggaa gttaactgga tccataatac acggagctgg
     8461 ggtgtcaaaa tggataatac ggcactcagt caggacggag ctacaaatat tgctgaagtc
     8521 aaaacgggtg ttcagggtaa attgtcagat aacctgaatg tctggggcaa tgttggtgtt
     8581 caggctggcg ataaagggta cagtgatgcc caggcaatgc tgggtattaa atatatattc
     8641 taatcacaca gtggcaggag gggacctcct gccacctcag ggttgtcata atgagttctg
     8701 taatgaaatt ttctgttgtt aatgcagtag tgctgctgtt gctgattgta cttactggtg
     8761 ttttttatat gaaaaagtca aaagaaattg cagttgttaa tatcgatgct atttataatc
     8821 agtctgccgc cggagacatg gtcagaaagc atctgaatga ggttaagagt attcttgaaa
     8881 aaggctatga ggatgcgcaa aaagcattcg cagataacgc gaataaagag gcgctgcttc
     8941 ttgatgccag acaaaaactg gagcgacaat atgttattga ggaaaatagc gcaaaaatgg
     9001 tgttatcccg tgcgatagat aatgccatag gtacatggag aaaagacaat cctaaaaata
     9061 ttgctgtatt gtcttcaggt gttgtgcttg cgcataacga gtcagctgat atcaccaaag
     9121 aagtcatggc tctggttgaa aaagaaaatc tggtttttaa tcagttaccc aaagtggaag
     9181 tgcacaataa gaatgaagag aaaccagaac aaagtaagta atcactagat tctgacgatt
     9241 tccagaaggg agattcagca ggccagcatc tgtaatcatt cagatttgct ggcttgctgc
     9301 taaatatggt tgctaagaaa aatagcaggc cagtaattta ttcagattac tgaatataat
     9361 aatttttcag gaacggttat gctgaaggta gtatgtttaa tcgacacaat tatttgctgt
     9421 gtctaatatg aggtgacatt tttatgatca ctgaccagca agactgaccc gtattgatta
     9481 gcctgattat tggcaactta ttggggtagt cattttgaca tcgaatgggg gaggtttcca
     9541 gttaagttca cgaatgcatg atgatttgcg ttcacatatc gaagtactgg ttgactatac
     9601 taaggatttt aagataactc actgattgta agtgggttat tgcgaggggg atgatagatg
     9661 gtgtcccctg caggaatcga acctgcaatt agcccttagg aggggctcgt tatatccatt
     9721 taactaagag gacattgttg cgtattgatt ttggttgttc accatatgac tgataatacc
     9781 gtctttatgg ttttaaaatc aaggtgtaag ttcgtttttg ttcatggttg attatcttct
     9841 tttgcgttga aaagtgtttt gttcacttgc taatgcgtac atattgagta cagaatacat
     9901 tataaacttg tgtacaggat ataaagcatt ggcactgaac gacaccaaac tacgaggcct
     9961 ctacggaaaa ccctactctg gccctgctga aatcaccgat ggtgacgggt taagtgtgcg
    10021 gattactcca gcaggaacta tcacgtttca gtaccgttat cgctggaatg gtaagccagt
    10081 acgtcttact gtcgggcgct atccgtctac ttcactgaag gacgcgcgtg ttatcgtcgg
    10141 tgagatgcgc gcattgtaca tgaagggagt taaccctaaa aattattttg cgcccagtga
    10201 cggcgaactg acattaaaag aatgtctgga tcagtggtgg gataagtatg ttactgatct
    10261 gaaacccaat acacagacgc tttacagatc cgtcgtgtac aacactatgt acacacagtt
    10321 tgaaggctcg ccagttgcca gtatcccagt atcggcatgg gctcgtttct ttgacaaaca
    10381 agaaagcctc aacaagaaaa aggctcgtgt tttgttgtta caactgcgtt cagttattaa
    10441 ctggtgtatc agccgccagt taatcccatc atgcgaatta ctcaagctta gtgttaagaa
    10501 tattgggaaa aaaccagatg tagggagtcg tgtacttacc tatacggaac tggcaaaaat
    10561 ttggctggcg ctggagaact caaaagtatt tacttccaac aaggtgttac atcaattgct
    10621 attgctatgg ggggccagac tttctgagct acgtctcgcg acagccagtg agttcaacat
    10681 ggaagatctg gtctggacaa ccccaaaaga gcattcaaaa atggggaata ttatccggc
//
LOCUS       DAZFFE010000133        10648 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFFE010000133 DAZFFE010000000
VERSION     DAZFFE010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.133"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            156..431
                     /gene="ykgM"
                     /locus_tag="ACHJ69_004124"
     CDS             156..431
                     /gene="ykgM"
                     /locus_tag="ACHJ69_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO5016995.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKRGHDESP"
     gene            418..558
                     /gene="ykgO"
                     /locus_tag="ACHJ69_004125"
     CDS             418..558
                     /gene="ykgO"
                     /locus_tag="ACHJ69_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO5016996.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(593..818)
                     /gene="ykgL"
                     /locus_tag="ACHJ69_004126"
                     /pseudo
     CDS             complement(593..818)
                     /gene="ykgL"
                     /locus_tag="ACHJ69_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
     gene            1642..2184
                     /gene="ecpR"
                     /locus_tag="ACHJ69_004127"
                     /gene_synonym="matA"
     CDS             1642..2184
                     /gene="ecpR"
                     /locus_tag="ACHJ69_004127"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO5016997.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            2258..2845
                     /gene="ecpA"
                     /locus_tag="ACHJ69_004128"
                     /gene_synonym="matB"
     CDS             2258..2845
                     /gene="ecpA"
                     /locus_tag="ACHJ69_004128"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO5016998.1"
                     /translation="MKKKVLAIALVTVFTGTGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            2902..3618
                     /locus_tag="ACHJ69_004129"
                     /pseudo
     CDS             2902..3618
                     /locus_tag="ACHJ69_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3644..6169
                     /gene="ecpC"
                     /locus_tag="ACHJ69_004130"
                     /gene_synonym="yagX"
     CDS             3644..6169
                     /gene="ecpC"
                     /locus_tag="ACHJ69_004130"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO5016999.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASATDISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMTLANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTFSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSGTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIDASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            6159..7802
                     /gene="ecpD"
                     /locus_tag="ACHJ69_004131"
     CDS             6159..7802
                     /gene="ecpD"
                     /locus_tag="ACHJ69_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO5017000.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGRRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            7771..8481
                     /gene="ecpE"
                     /locus_tag="ACHJ69_004132"
     CDS             7771..8481
                     /gene="ecpE"
                     /locus_tag="ACHJ69_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO5017001.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            8794..9123
                     /gene="ykgJ"
                     /locus_tag="ACHJ69_004133"
     CDS             8794..9123
                     /gene="ykgJ"
                     /locus_tag="ACHJ69_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO5017002.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(9135..9301)
                     /locus_tag="ACHJ69_004134"
                     /pseudo
     CDS             complement(9135..9301)
                     /locus_tag="ACHJ69_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(9372..9986)
                     /gene="yagU"
                     /locus_tag="ACHJ69_004135"
     CDS             complement(9372..9986)
                     /gene="yagU"
                     /locus_tag="ACHJ69_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO5017003.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNSAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(10201..10386)
                     /locus_tag="ACHJ69_004136"
     CDS             complement(10201..10386)
                     /locus_tag="ACHJ69_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017004.1"
                     /translation="MASGLICHGGFEPHAWGVANLMDVAVLVVAYLLVWLLVNRENHY
                     ARGIPQDVQVLHKKAHH"
     gene            10435..10594
                     /locus_tag="ACHJ69_004137"
                     /pseudo
     CDS             10435..10594
                     /locus_tag="ACHJ69_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         2911 a         2625 c         2543 g         2569 t
ORIGIN      
        1 cgtttatggg agcgtcgcca atggctgcta acaatgccag acttccccgt tgcggaaatt
       61 ccacatccca caaatagtca cagtgattgg gtgttgaaat gatccggatg agcatgtatc
      121 tttacggtta tgttataaca taacaggtaa acatgatgaa acccaatatc catcctgagt
      181 atcgtactgt ggtgttccac gacaccagcg ttgatgagta ctttaaaatc ggctcgacta
      241 tcaaaacaga ccgtgagatt gagctggatg gcgtaacgta tccgtacgta acaattgatg
      301 tctcttcgaa atcgcacccg ttctatacag ggaagctgag aacagtggca tcagaaggaa
      361 atgttgcacg gttcacccaa cgttttggtc gttttgttag cacgaaaagg gggcatgatg
      421 aaagtcctta actctctgcg taccgcaaaa gaacgccatc cagactgtca gattgtgaag
      481 cgaaaaggac ggctgtatgt gatttgtaaa tctaatccac gttttaaggc cgttcagggt
      541 cgtaagaaaa aacgttgatt taaaattcga cgaattaacg atatttatct gattaataat
      601 cagaccggat taatgttggt gtatttatta caccagcatt aattttcctg gggatgtatc
      661 ttcctgttca tttaaggtca actgcctgac gtttctctcc gaatattcca ttatattaat
      721 gttgacttgt tgaccagttt cgccctgtat gctggcatca actctctttt agactgaaca
      781 cgccactcag tttcctccct ttgcgacgca gcctgcattt acactcaaac tgttaagatg
      841 ataaatgtgg taaatctgtt ggtactgaca taaaaacgtt tacgccacag gaacggccag
      901 atccatcggt aaccccatcg ccgacgttcg agtgccagtt agagtaacgc gcacagatga
      961 ctgaatgcag tgccctggca aagaggccat cgttcctgtg acaactggca gtcttcgttt
     1021 aacttcactt aatttggctc ttggggggct taccggacag atgacgtact tacacctgtt
     1081 taatttttca tcacttattg ggatgaacac ccataaccat tttgtgcggc atgttaatcc
     1141 attaaaacac cttactgatt ggcaaatcat ctttaattat gacttatgat agttttatat
     1201 tctatttctt gtcatttaaa cttgtttttt tactagtcca ttacacaaca cattaagact
     1261 attcctaaca cctcagggca aagttcctgg ctaatataaa atgcaagtaa gaattgaacg
     1321 ttatattgcc aataacctta tgaaactgaa tgtctttttc ttcttatcaa aaaagcaata
     1381 ttttcatttt ttgtaaatat tgacttaacc atggaattca ttttctgttc acatattgac
     1441 actcatcagg aaaaaaacat aaatttaaac ctaatcgaaa taattaaaac ttaatctcgt
     1501 ttaacctata ttgatatgtg ctacgtatct tatttacttc cgatttacta aagaaactga
     1561 atgtacctgt aaaaattaca ggtttggaaa gtagtgacat ggcaaaatga ttacagcagg
     1621 gactatgagg ttaaaaacca tatggaatgt caaaaccgtt ctgataaata catctggtct
     1681 ccccatgacg cctacttcta taaaggacta tctgaactga ttgtggatat cgacagatta
     1741 atttatctat cgctggagaa aatcagaaaa gatttcgtgt ttatcaatct caatacggat
     1801 tctttaactg agtttataaa ccgtgataat gagtggttat ccgcggtaaa ggggaaacag
     1861 gtcgtattga ttgcggccag aaagtcagaa gccttagcaa attattggta ttacaacagc
     1921 aatattaggg gcgtggtata cgctggactg agtcgtgata ttagaaaaga actggcctat
     1981 gtgattaatg gcaggttcct gagaaaagat attaagaaag ataaaatcac tgaccgggaa
     2041 atggaaatta tccgcatgac ggctcaggga atgctgccta aatcgattgc cagaattgaa
     2101 aattgtagtg tgaagacagt gtatacccat cggcggaatg cagaggccaa gctgtactca
     2161 aaattatata agttggttca gtaaactcca ggcaagttag ttttaaaaat gactcaccgg
     2221 gacttcatgt cctcaattca actcgggaag aaaagcaatg aaaaaaaagg ttctggcaat
     2281 agctctggta acggtgttta ccggtacagg tgtagcgcag gctgctgacg taacagctca
     2341 ggctgtagcg acctggtcag caacagccaa aaaagacacc accagtaagc tggttgtgac
     2401 gccactcggt agcctggcgt tccagtatgc cgaaggcatt aaaggtttta actcacaaaa
     2461 aggtctattt gacgtggcta tcgagggtga ctcaacggct accgccttta aactgacctc
     2521 acgccttatc accaacacct taacccagtt ggatacctca ggttccacac tgaatgtggg
     2581 cgtggattat aacggcgcgg cagtcgaaaa aactggcgat accgtgatga tcgataccgc
     2641 caacggcgta ctgggcggca accttagccc actggctaac ggttacaatg ccagcaatcg
     2701 taccaccgca caggatggtt tcactttctc catcatcagc ggcaccacca atggtaccac
     2761 cgcagtaacc gattacagca ctctaccgga aggcatctgg agcggcgacg ttagcgtaca
     2821 gttcgacgct acctggacca gttaatctct ctgatgtacc agcaggggta cccccctgct
     2881 ttattcctgg acggaattgt tatgaaaaag caccttctgc ctctcgctct gctgttttcc
     2941 ggaatatcac cggcccaggc gctggatgtc ggcgatatat catcgtttat gaacagtgac
     3001 agcagcacgc taagcaaaac gatccaaaac agtaccgaca gtggccgcct catcaatatc
     3061 cgtctcgaac ggctctcttc accgcttgac gacgggcagg ttatcgcaat ggacaagccg
     3121 gatgagttgc tactcactcc cgccagcttg ctgctacccg cccaagccag cgaagtgatc
     3181 cgcttcttct ataagggacc agcagatgaa aaagagcgct aagtgatccg cttcttctat
     3241 aagggaccag cagatgaaaa agagcgctac taccgcattg tctggtttga tcaggccctc
     3301 agtgatgcgc agcgcgataa tgccaaccgt agcgctgtgg ccactgcttc cgcccgcatc
     3361 ggcaccattc tggtcgtcgc accccgccag gcaaactacc actttcagta cgccaacggc
     3421 tccctgacaa atacaggaaa tgcgacgctg cggatcctcg cctacggccc ctgcctgaaa
     3481 gccgccaatg gtaaggagtg taaagagaat tactacctga tgccgggcaa gtcgcgtcgt
     3541 tttacccgcg tggacacggc ggataacaaa ggacgggttg cactttggca gggtgataag
     3601 ttcattcccg tgaaatagat agctgtgcag atggataacg acaatgcctt tacgacggtt
     3661 ctccccagga ctgaaagccc agtttgcctt cggcatggtc tttttgttcg ttcagcccga
     3721 tgccagcgct actgacataa gtgcgcagca aataggtggg gtgattattc cgcaggcctt
     3781 cagtcaggcg cttcaggacg gcatgagcgt cccgctctat attcatctcg ccggtagcca
     3841 gggtcgccag gacgatcagc gaatcggcag cgcttttatc tggttggatg atggacagtt
     3901 acgcatccgg aaaatacagc tggaagagag tgaagataac gccagtgtca gcgaacaaac
     3961 tcgacagcaa ctgatgactc tggccaacgc cccgttcaat gaggccctta ccatccccct
     4021 gactgacaac gcgcagttgg atctcagctt gcgccaactg ctgctgcagc tggtggtcaa
     4081 gcgcgaagcg ctgggcaccg tactacgctc acgtagcgaa gacatcgggc agtccagtgt
     4141 taacaccctc agcagtaatc tgagctataa cttcggcgtc tataacaacc agttgcgtaa
     4201 cggcgggagc aacacctcca gctatctgtc gctgaataac gttactgcgc tgcgcgaaca
     4261 tcacgtggtg ctcgacggct cactgtacgg gattggtagc ggtcaacagg acagtgaatt
     4321 atataaagcg atgtatgaac gcgattttgc tggtcaccga tttgccggtg gaatgctcga
     4381 cacctggaac ttgcagtcct tagggccgat gaccgccatt tcagcaggga agatttacgg
     4441 cctttcctgg ggaaaccagg ccagctccac catcttcgac agcagccaat cggccacgcc
     4501 agtgatcgcc tttttaccgg cggcgggcga agtacatctc acccgtgatg ggcgactact
     4561 aagcgttcag aacttcacca tgggcaatca tgaagtggat acccggggtc taccgtacgg
     4621 tatttacgat gtggaagttg aggtaatcgt taacggtcgc gtgatcagca aacgcaccca
     4681 gcgggtcaat aagctgttta gccggggacg tggcgtcggt gcaccgctgg cgtggcagat
     4741 atggggcggt agctttcata tggatcgctg gtcggaaaac gggaaaaaga cgcgaccagc
     4801 caaagagagt tggctggcag gtgcctcgac ctccggctca ctgagtacgt ttagctgggc
     4861 ggcaacggga tatggatacg ataatcaggc ggtgggtgaa acccgtctga cgctgccgct
     4921 tgggggggcg atcaacgtta acctgcaaaa catgctggcc agtgacagct catggagcaa
     4981 catcgccagc atcagcgcca ctctacctgg aggcttcagt tcgctgtggg ttaaccagga
     5041 aaaaacccgc attggcaatc aattgcgacg tagcgatgcc gacaaccgtg caatcggcgg
     5101 cacactcaac ctgaactcac tgtggtcgaa gctgggtacg ttcagcatca gctacaatga
     5161 tgaccgccgc tacaacagcc attattacac ggcagattac tatcaaagtg tctacagcgg
     5221 tacctttggt tcgcttggcc tgcgggccgg tattcagcgc tataacaacg gcgacagcag
     5281 cgccaataca gggaaatata tcgctctcga tctctcgcta ccactgggca actggtttag
     5341 cgcagggatg acccatcaaa acggctacac catggcaaac ctgtcagcac gcaaacagtt
     5401 tgatgaagga accattcgca ctgttggtgc caatctgtca cgagccatct ccggcgatac
     5461 cggtgatgac aaaaccctca gcggtggggc gtatgcacag ttcgacgctc gttacgccag
     5521 cggaacgctg aacgtcaata gcgcggcgga cggctacatc aatactaatt tgaccgccaa
     5581 cggcagcgtc ggctggcagg gtaaaaacat cgatgccagc gggcggaccg atggcaacgc
     5641 tggggtgata ttcgacactg gcctggagaa cgatggtcag atcagcgcca aaatcaacgg
     5701 gcggattttc ccgcttaacg gcaagcgtaa ctatctcccg ctctctccct atggaagata
     5761 tgaggtggag ttgcagaaca gcaaaaactc actcgacagt tacgatatcg tcagcggtcg
     5821 caaaagtcat ctgactctct atccaggcaa tgtcgctgtc attgagccag aggtgaagca
     5881 gatggttacc gtctccggtc gtatccgtgc ggaagacggc acactgctgg ctaacgcacg
     5941 gattaacaac catatcggcc gaacccgaac cgatgaaaac ggcgagtttg tcatggacgt
     6001 ggataagaaa taccccacta tcgattttcg ctacagtggc aataaaacct gcgaagtggc
     6061 actggaactc aaccaggcgc gcggtgccgt ctgggtcggt gatgtggtct gcagcggcct
     6121 ctcatcgtgg gcggcggtga cgcagacagg agaagagaat gagagttaac ctactgatag
     6181 cgatgataat ctttgcgcta atctggcctg caaccgcgct cagagcggca gtgagcaaaa
     6241 caacctgggc ggatgctccg gcacgcgagt ttgtgtttgt cgaaaacaac tcagacgaca
     6301 actttttcgt cactcctggc ggggcgctgg atccgcgcct gaccggtgcc aaccgctgga
     6361 ccggtttaaa atacaatggt tcaggaacca tctatcagca aagcctcggc tacattgata
     6421 acggttacaa caccggcctt tataccaact ggaagtttga tatgtggctg gaaaattcac
     6481 cagtttcatc tcctttaact ggcttgcgct gcatcaactg gtacgcaggg tgtaatatga
     6541 ccaccagtct tatcctgccg caaaccaccg acgccagtgg attttatggc gcgacggtga
     6601 ccagcggcgg cgcgaagtgg atgcacggca tgttgtcaga cgcgttttac cagtatctgc
     6661 aacaaatgcc cgtcggcagc agctttacaa tgaccatcaa tgcctgccag acctctgtga
     6721 actatgacgc cagcagcggc gcacgctgta aggatcaggc ctccggcaac tggtatgttc
     6781 gcaacgtcac ccatacgaaa gcagcaaatc tacggttgat aaatacccac tcgctggcgg
     6841 aagtatttat caacagcgac ggagtaccga ctctgggcga agggaacgcc gactgccgga
     6901 cgcaaaccat cggccgtcgt tcaggattaa gttgtaagat ggttaactat accctgcaaa
     6961 caaacggact cagcaacacc tcaatccata tattcccggc gatcgccaac tcgtcgttag
     7021 cctcggccgt cggggcgtac gatatgcagt tcagtctgaa tggcagttca tggaaaccgg
     7081 tgagcaatac tgcctattac tacaccttca acgagatgaa gagcgcagac tcgatctatg
     7141 tgttcttctc gagcaacttc tttaagcaga tggtgaacct cgggatcagc gatatcaaca
     7201 ccaaagatct attcaacttt cgctttcaga acaccacatc accggagtct ggctggtatg
     7261 aattttctac ctccaacacg ctgattatca aaccccgtga tttcagcatc agtattatct
     7321 ccgatgaata tactcagaca ccgtcgcggg agggatatgt tggcagcggc gagtcggcac
     7381 tcgatttcgg ctatatcgta accaccagcg gtaaaacagc tgccgacgaa gtgctgatca
     7441 aggtgaccgg acccgcgcag gtgattggcg ggcgctccta ttgtgtcttc agctccgatg
     7501 acggtaaggc gaaagtaccg ttcccggcga cgctttcctt tattacccgc aacggagcta
     7561 caaaaaccta cgatgccggg tgcgatgata gctggcggga tatgaccgat gcgctgtggt
     7621 tgaccacacc gtggactgat atctctggcg aagtggggca gatggataag accacagtca
     7681 aattttcgat tccaatggat aacgccattt ctctgcgtac ggtagatgat aacggctggt
     7741 ttggcgaagt cagcgcttca ggagagattc atgttcaggc gacgtggcgt aacattaact
     7801 aaggccctgc tgacagtggt ctgtatgctg gcggcacctt tgacacaggc gatttcggtc
     7861 ggcaatctga ctttttcact gccgtccgag actgactttg tcagcaaacg tgtcgtgaat
     7921 aacaacaaaa gcgcgcgaat ataccgtatt gccatcagtg ctattgatag cccgggcagc
     7981 agtgaattgc gcacccgacc ggtggatggt gaactacttt tcgccccccg ccagctggcg
     8041 ttgcaggctg gtgagagcga gtattttaaa ttttactatc atggtccacg ggataaccgc
     8101 gagcgctact accgggtctc atttcgcgag gtccccactc gtaacctgac aaaacgcagc
     8161 ccaaccggcg gtgaggtcag cacggagccg gtggtggtga tggataccat tctggtagta
     8221 cgaccacgtc aggttcagtt taaatggtcc ttcgatcagg tgaccggaac ggtaagtaac
     8281 accggcaaca cgtggtttaa gctactgatt aaaccaggat gtgattcgac cgaagaggaa
     8341 ggcgatgcct ggtatctacg tccgggagac gtggttcatc agcctgagtt acgtcagccg
     8401 gggaatcatt atctggtcta taacgacaaa ttcattaaga ttagcgattc ctgtccggct
     8461 aagccccctt cggcggacta agacttatcc atcagacgag ggaagaattc cacctcagag
     8521 actcaaaaat cgtttaaatg atgtggaagc gatcgtcaat gtcacctgta acgatgagct
     8581 ttctcatatg cacaccacag tgttcaggag acacggaaag gtaccaggcg gcctgctaac
     8641 gagtggtttc ttcactaatt aggacgtaaa atcggtaacg gctggaaatc attcaatact
     8701 cacactatcg aaagtacacc agccaaccgc gatacgtccc tgcatacgac gtaccgcagt
     8761 tctctttacg atttatagct gtactgatga attatgagca atctgaatcc atgcatgacg
     8821 tgtggtgcct gttgtgcatt tttccgcgtc tctttttact gggccgaagc cgacgatgct
     8881 ggcggtacca ttcccgccag gcttactgaa caaatatccc cttttcaccg atgcatgagc
     8941 ggtaccaatc agaaaaaccc ccgatgtatt gcccttgcag gaacaccggg caaaaatgcc
     9001 tgttgcacga tatataaaaa tcgatcgtcc acatgcagag aattcgccat gtctggtgag
     9061 aacggagaag tcaatgaggc ttgcaatcgt gcaagagcta aatacgggct aacaccgtta
     9121 taaatataca ataattaatt gcattgcccc gccagctgta atagcggggc ttcgtttttc
     9181 agggacaaca aaacctgaaa cttcttccat tttccagcaa caatgcctct tcttctgtag
     9241 cgttctccgg atctacatca tcctgaagta gcgcgatatg atcactgact gtccgcatca
     9301 gaatatgaaa agcaacgttt ttaacctata aaaatggcgc tatatttgcg ccatttttat
     9361 cattcaaagc attatctatt tgagcctaaa gggatctcag gatctggctc atgggtaatt
     9421 ctgtttcgta aatctctgcg aataatttca atagaccaga accagactaa atgtccaaaa
     9481 atttcagaga cattctcata ccacggaaga tcaaagagag gtggcgtaag tcccatgaga
     9541 gggaatgaaa tcatatgaac aaaaagttgc gctaaagcac ctgccagtaa gccctgccaa
     9601 agtttaattt tcggaaatac ttcggcgacc acacagtaac caacggcaaa aactatcgaa
     9661 aagattatgt gcgttacacc aacccagtta aagacatgcc cggcgaaggt ataaacagcc
     9721 gaatttggat ctgtcagtcc caaccaatcc cgaagaaaaa tgtacggagg attgagaaag
     9781 ttacgggagc aatcaatttg gcctgcagcc ctgattaatg attccggacc acacgctgca
     9841 ttaaacatat caaccggact acgtggtggc aatggaactt ctgcccccca cttaacaaat
     9901 gcggaaacaa cgccagcaat cagcccaatg aatgcagcaa gaccataacg tctgcggttt
     9961 ggtggagttt gttcaaatat attcatatct accctgcttg taccattatg ttatacacct
    10021 ctttaggagt attcataaaa caaggcaaat gtaaagaact gtattgtttt gtataaccac
    10081 atggttacct aatcgccaat taatataagc tccattattt ctccatattt ttatgttaga
    10141 agtgacaagg agaaatgtaa tgtttctctc agagaatata aatcattcct tttgcccacc
    10201 ttaatggtgg gcttttttat gcaaaacctg aacgtcctgt ggtattcctc tggcataatg
    10261 attttcgcga ttaaccaaca accagaccag caaatacgcc accaccagca cagcaacatc
    10321 catcaggttt gccacacccc atgcgtgcgg ctcaaagccg ccatggcata ttaatccgct
    10381 tgccattttc gaacagctga taggtttagt ccggtgtttc cctgcttgca cttaaaaatg
    10441 agcggatggg aaactctcga aatggttcgt cgatatgctc acctcgcacc taatcacctt
    10501 accgaacacg cacggtaaat agattcgatc ctgaacccat cggtcccaaa tctgtcccaa
    10561 tcaaaaaaat aaggaaggta ttaatgatgt gtaacttatt gatttaaaat ggataatata
    10621 aacagattgt aaaaagcaag acattgat
//
LOCUS       DAZFFE010000134        10619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFFE010000134 DAZFFE010000000
VERSION     DAZFFE010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.134"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..359)
                     /locus_tag="ACHJ69_004138"
     CDS             complement(<1..359)
                     /locus_tag="ACHJ69_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HFO5017005.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFS"
     gene            complement(425..787)
                     /gene="yfdP"
                     /locus_tag="ACHJ69_004139"
     CDS             complement(425..787)
                     /gene="yfdP"
                     /locus_tag="ACHJ69_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFO5017006.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            complement(1454..2128)
                     /gene="ymfK"
                     /locus_tag="ACHJ69_004140"
     CDS             complement(1454..2128)
                     /gene="ymfK"
                     /locus_tag="ACHJ69_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HFO5017007.1"
                     /translation="MKTVHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            2219..2419
                     /gene="ymfT"
                     /locus_tag="ACHJ69_004141"
     CDS             2219..2419
                     /gene="ymfT"
                     /locus_tag="ACHJ69_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFO5017008.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            2463..3020
                     /gene="ymfL"
                     /locus_tag="ACHJ69_004142"
     CDS             2463..3020
                     /gene="ymfL"
                     /locus_tag="ACHJ69_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFO5017009.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAAEWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSRQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCAPE
                     NSNARECAAPGVVASIASGCGETNA"
     gene            3166..3345
                     /locus_tag="ACHJ69_004143"
     CDS             3166..3345
                     /locus_tag="ACHJ69_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5017010.1"
                     /translation="MQELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYQHECFSPLW
                     KFRRDFVECEGPPAH"
     gene            3275..4177
                     /locus_tag="ACHJ69_004144"
     CDS             3275..4177
                     /locus_tag="ACHJ69_004144"
                     /inference="COORDINATES: protein motif:HMM:TIGR02681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO5017011.1"
                     /translation="MSALVLCGNSGVILLSVKDHQHIDSAIPGRYTVQAPYKAGAGIG
                     VLKLSTAMYDAPASFLSSAFAHIQIMVGWAGAPKGAPVSVDAGYANPVQFTTSEIGVS
                     GGGSFSLSTEAAIMATIPALTQPEITIDNGQAVTTSLAVANSFSKRHDDVLKKIRTLD
                     CSPEFCARNFAETSISVNQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYIN
                     AFNQMEKLLSKPSTLSDAADNASVLYSHLSVIHKVWLQQLYPMLAKAESPLAVSLYDR
                     INDAALLASLINLSLNPSEVRGRK"
     gene            4174..4398
                     /locus_tag="ACHJ69_004145"
     CDS             4174..4398
                     /locus_tag="ACHJ69_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017012.1"
                     /translation="MIRNIFKRFTNQTFRCPRPGQWYTTPAGHVLRVSLVDRECQKVI
                     CEPLGRNYRISMPLIAFCSGKMFKRLGGVA"
     gene            4395..4694
                     /locus_tag="ACHJ69_004146"
     CDS             4395..4694
                     /locus_tag="ACHJ69_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5017013.1"
                     /translation="MNCFQFVCGCAFDNPIQRLIMLRVLMSGSSDGEGERVIDHQVLA
                     DFCCCSKQAIFRETLALERAGYLHIRKIATLTIDAKARLQPARGYTILMPRKEVV"
     gene            4691..5674
                     /locus_tag="ACHJ69_004147"
     CDS             4691..5674
                     /locus_tag="ACHJ69_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO5017014.1"
                     /translation="MSRYAPTPEVMAIGQINISGNVTPANWWKYIRLPSGRPDATAIA
                     LLSEIVYWYRPTEVRDEHTGALLGYRKRFQGDKLQRSYQAFAEQFGFGKRETADALKR
                     LRDAGFITLDLRTVEMLDGVKCSNILFVGINPQAIAAITTPSSVSPESNSNNAISDTA
                     ITLKRNTPRRHNGTGDTLNVDTNTEITTEITTETKNTIDASADASAPARSARQEYSPE
                     FEQAWQEYPKRAGGNSKSAAFKAWKARIREGVTPETMLDGVKRYAAWVRVSGNTGTQF
                     VKQASTFFGPDRHFEESWEVPAVSAARREDPYFKASYDNVDYSQIPAGFRG"
     gene            5671..5754
                     /locus_tag="ACHJ69_004148"
                     /pseudo
     CDS             5671..5754
                     /locus_tag="ACHJ69_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5769..6422
                     /locus_tag="ACHJ69_004149"
     CDS             5769..6422
                     /locus_tag="ACHJ69_004149"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO5017015.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHATAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMVA"
     gene            6419..6814
                     /locus_tag="ACHJ69_004150"
     CDS             6419..6814
                     /locus_tag="ACHJ69_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5017016.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKASCKLATR"
     gene            6977..7792
                     /locus_tag="ACHJ69_004151"
     CDS             6977..7792
                     /locus_tag="ACHJ69_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO5017017.1"
                     /translation="MNQLLVIDGVSVRQYFESNYCLNDLQKAALLAAGENRSSRSLEV
                     HEFMRRPETKALVELLEEETTGDSRSIPVITIQGRNGGTYVCKELVYAYAMWISPAFS
                     LKVIRTFDALHNSSPEETTSDKIKSGVILLESAAKTLNLSNSSKLGAYQKLSKVAGLP
                     ELMPIYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYS
                     RTAINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            7800..8789
                     /locus_tag="ACHJ69_004152"
     CDS             7800..8789
                     /locus_tag="ACHJ69_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5017018.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRSVFRHEAVIRRAGGVECLESWLLREKGCQWPHSGWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKVLRLPKTVVPSVTRESDLVPSVPATSIIRDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MATKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            8804..9199
                     /locus_tag="ACHJ69_004153"
     CDS             8804..9199
                     /locus_tag="ACHJ69_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO5017019.1"
                     /translation="MMRDIQMVLERWGAWAASGNAGVDYSPIAAGFKGLLPSTAKPRP
                     ACSDDDGLIIENCLTRLKKKKPDEYSLLVAHYLLRISKRQIARTRKKSEKAIRIEMQI
                     AEGFIDGCLSMLGVRLEMDDWLPKKVKND"
     gene            9404..9601
                     /locus_tag="ACHJ69_004154"
     CDS             9404..9601
                     /locus_tag="ACHJ69_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO5017020.1"
                     /translation="MLKQQDMTETARVVFNELSATEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            complement(9784..9915)
                     /locus_tag="ACHJ69_004155"
                     /pseudo
     CDS             complement(9784..9915)
                     /locus_tag="ACHJ69_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2616 a         2509 c         2793 g         2701 t
ORIGIN      
        1 gagaacggtg ctgtcttttt cagtttgaga gtggcggtgt tatctgcgtg acctggttca
       61 tcaatagtac ccaggttaag cacactgacg gcacgcatat tatcagcatc gataaagcag
      121 cgggtgcctt catctgcaag atctttagaa taacgggtaa agtcatcgat gctggcagtg
      181 gaaagcgcac cacggaaacg gaagcgattt aaattaaatt tttccagatc atgaatgcgg
      241 aaattctcag gcaatgccac agcatcggca ccaatcttac tgataatttc attaacaccc
      301 tgcgcagaaa taagggcatg gatttgatta attgcggttg cgtctaagtt ctgagacata
      361 ataagtcctc actatataaa gatattcagt gatgagataa ataatcagtt aattaagaac
      421 gatattaatg acctgctgcg cggagttttc cgtcaggttc accggcaaga gtcagtaatt
      481 gtccctggtc ttcctgcaga atagtcaggc gaccaccgcg attgacatac atcggcgttt
      541 cggtggtgtc ttcttcggaa attttcccgc ggttagtcgg acgaacatat gagagtttgt
      601 gttttatttt cacacggttc tcatcaaacg gttcgatttc caggttgagc gagaccttcc
      661 ctttggtttt cgtgttcatc acacctgaag cgacttcact gagaacagcg ccgattttgg
      721 tttcaaatac gccgccgtcc agctccccga taaatgcctg cacatcagta ctgcgttcgc
      781 tagccatttt gctgctcctc atcatatcga ccctgcaagg tcggttggtt tctccacaaa
      841 acagagaaga acacctgcgg tggcagccgc ccgggtggat tgggttatga gcccgtcgtc
      901 cggtgatgct cttctctgtt ttgtaaaaag agcggtacca gccggaagca agtgtacaaa
      961 ctggtaccgc caaagcagtg gctgttgtgg tgggcttgtc acttaagcgt atggtcaacc
     1021 tgacaacccg gtgtcctcaa cggggaagga ataaccccgc catacttacc gccgcgccat
     1081 ttcgcggatt accacaacgc tgagagcact tagccagtta cggcaccata ctttgtcgcg
     1141 gttccataaa tgccctcatc gttgcaccct ggtctcttcc caggcgtcaa accgaatcgc
     1201 cacgctggtt aggcgtctta tcagcatcct cattgacttg cacattccgg ctacctggtt
     1261 tgtttgcccg agcaaggagt ggattgtccc ctttaacgtc cccagaccgc taacgacgca
     1321 tgtgccatac gccgtgttac aaccaaattt tgtttaatct tgcctgtgac atgtttcttt
     1381 tagatacatt atgtatctca agggtacatt gtcaagtata aaaaaacctg ccgaagcagg
     1441 ttataaatat tgattaggcc tttattttgt atcttcttgg ttttcctgag aaaattactg
     1501 tgccaattat agagcaatta ccgttgatct taatgtaagg ttcaggccag tttgggttta
     1561 atgctttgag gtaacgctgt gttccatctt ctatcaaccg cttaaaggtg gtttcgcctg
     1621 tatcgtgcat caatgcaata acgtcgtcac cgtggcaggc agggacttca ggatctacaa
     1681 aaatcatgtc tcccgggcgg tactcatcaa tcattgaatc accaatcacc cgcaagatat
     1741 aagtcatttc gccacagggt acagggcagg gataagtttc tgctgtgctc aaatcaacct
     1801 cagaatagcc aacttctttc catgctccgg cctgtaccca tgatatgaca gggactaacg
     1861 ttatttgttt gttagtaatt gaaacatcag gtttttttgt gatgtttgtt gtctggtgtt
     1921 cttgatcaag ccatccgaca ggcaggtcga aacatttttc gatgtgccgt gccatgctgt
     1981 caccgatatt tttagtagca ccatctccca taaacctgct ggtctgggtt ggctcgcgat
     2041 caatcatggt ggcaaaggaa gaattcccgc caacaccatc tctcagtttt ctggcgttag
     2101 accgccggat gtcatggact gttttcataa cgaaattaaa acctttgtac cgatagggta
     2161 caagtatctt gaaggttcat ctcaatcatg taatatgtat atcggaggta catattgtat
     2221 gaaagcgtat tgggactctt taaccaaaga acagcagggc gagttggccg gaaaagttgg
     2281 ctcaacacca ggctacttac ggctggtttt caatggttat aaaaaagcca gttttgtgct
     2341 ggctaaaaaa cttgagcaat gcacgtcagg tgcaattacg aaatctgact taagaccgga
     2401 tatctatccg aaagattaac agaacacctt caatttttaa ccacagaacg atgaggctaa
     2461 ccgtgggtaa gcatcactgg aaagtagaaa aacagcctga gtggtacgtg aaagctgtca
     2521 gaaaaactat cgcggcgttg ccggggggtt acgctgaagc tgctgagtgg ctggatgtaa
     2581 cagagaacgc tttattcaat cgccttcgtg ccgatggcga tcagattttc ccgctgggat
     2641 gggcaatgat tttacaacgt gctggtggaa ctcacttcat tgctgacgct gtggcgcagt
     2701 ctgcaaatgg cgtctttgtg tctcttcctg acgtcgagga tgtggacaac gccgatatta
     2761 accagcgtct gctggaagtc attgaacaga tcggcagtta ttccagacag attcgttcag
     2821 caatcgaaga cggtgtagtg gaaccgcatg agaagacagc aattaacgac gagctgtacc
     2881 tctcaatttc gaagctccag gagcatgcag cactggtcta caaaatcttc tgcgctccag
     2941 aaaatagtaa cgcccgcgag tgtgcagctc cgggcgtcgt ggcgtcgatt gcttctggtt
     3001 gtggagaaac taacgcatga atagtttaac ggcaaataac cgtttgtcgc aacagctggt
     3061 ggtcagcgtc gctgaacacc tgttgttacg gcatgaatgc agattaccaa atcacctggc
     3121 tgtaagtaac cacagagaac tttacctgac tgtggggggc gagttgtgca ggaacttaac
     3181 cgctggtttc gtgacggaag aggactttat gttcatgtta ttcgttggga gccagaaaca
     3241 cagcgcgtta tctatcttcg caaagactac cagcatgagt gctttagtcc tttgtggaaa
     3301 ttcaggcgtg attttgttga gtgtgaagga ccaccagcac attgattctg ccattccggg
     3361 acgttacact gttcaggcac cttataaagc gggtgccggg attggcgtcc tgaaattgtc
     3421 aacggcgatg tatgacgcgc cagcgtcttt tttatcgtcc gcatttgctc acatccagat
     3481 tatggtgggc tgggcggggg caccgaaagg tgcgccggtc tccgttgacg ccggttacgc
     3541 caaccccgtc cagttcacca ccagtgaaat tggcgtttcc ggtggtggaa gtttttcact
     3601 gtcaacggag gctgccatca tggctacgat cccagccctc actcaacctg aaatcaccat
     3661 tgacaacggc caggccgtta ccacttcttt ggctgttgcc aactccttct ccaagcgtca
     3721 cgacgatgtg ctgaaaaaga tccgcactct ggattgttcc ccagagtttt gtgcccgcaa
     3781 ttttgcggag acatcgattt cggtaaatca accgaacggt ggtacacgca agctcccttg
     3841 ctatcaaatc acacgagacg gttttgcgtt tcttgctatg ggtttcacgg gtaaacgtgc
     3901 tgcccggttc aaagaggcat acatcaatgc ctttaaccag atggaaaaac tgctttcaaa
     3961 gccatccacg ctgagcgatg ccgcagataa cgccagcgtg ctttactccc acctgtcggt
     4021 aatccacaag gtctggctgc agcagcttta tcccatgttg gcaaaagccg aatccccgct
     4081 ggctgtaagt ctgtatgacc gcatcaacga cgcggcgcta ctggccagtc tcataaattt
     4141 gtcgctgaac ccttcagagg taagggggcg caaatgatcc ggaatatttt caaacggttt
     4201 accaatcaga ctttccgttg tcctcgtcct ggtcagtggt acaccacgcc tgcagggcat
     4261 gttctacgtg tcagcctggt tgaccgtgaa tgtcagaagg tgatttgtga accgctgggc
     4321 cgtaattacc gcatcagtat gccgcttata gccttttgct ccggaaaaat gtttaagcgt
     4381 ctgggaggtg tggcgtgaac tgttttcagt ttgtgtgcgg atgtgctttc gataacccga
     4441 ttcagcgcct gattatgttg cgtgttttga tgtcgggttc ttcagacggt gaaggcgaga
     4501 gagttattga tcatcaggtg cttgctgatt tctgctgttg ttctaagcaa gcgatattca
     4561 gggaaaccct ggcactggaa agagctggtt atcttcatat ccgaaaaatt gcaacgctta
     4621 ctattgatgc aaaagccaga ctacaacctg cgcgtggcta cacaattctc atgccgcgga
     4681 aggaggttgt atgagccgtt acgcccccac accggaagtt atggctattg gtcaaattaa
     4741 tatttccggc aatgttacac ctgcgaactg gtggaaatat attcgactac ccagtgggcg
     4801 tccggatgcg acggctatcg ctctgctttc agagatcgtt tactggtacc gcccgacaga
     4861 ggtcagggat gagcacaccg gagcgttgct gggatatcgc aagcgttttc agggcgacaa
     4921 actgcaaaga agctaccagg cgtttgctga gcagtttggt ttcgggaaaa gggaaaccgc
     4981 agatgcgctg aagcgtctgc gcgatgcagg gtttattact ctggatttac gcacggtgga
     5041 aatgctcgat ggggtgaaat gcagcaatat tttgtttgtc gggatcaacc cacaggcaat
     5101 tgcggccatc accacacctt cttctgtttc gccagaaagt aacagcaata atgcaatcag
     5161 cgatacagct attacgttaa aacggaacac cccccgacgt cataacggaa caggggatac
     5221 gctgaatgtt gatacaaata cagagattac tacagagatt acaacggaga ctaaaaacac
     5281 tattgatgca tccgctgacg cgtctgcgcc agcgcgttct gcccgacagg aatattcacc
     5341 ggaatttgaa caggcctggc aggaatatcc caaacgtgct ggtggtaatt caaaatccgc
     5401 cgcttttaaa gcctggaaag cccgaatcag ggaaggtgtg acacccgaaa ccatgctcga
     5461 cggtgtgaaa cgctatgccg cctgggtgcg tgtctctgga aataccggta cccagttcgt
     5521 gaagcaggcg tcgacgttct ttggtccgga tcgtcatttc gaagaatcct gggaagttcc
     5581 tgcggtatct gcagccagac gcgaggaccc gtacttcaaa gccagttacg acaacgtgga
     5641 ctacagccag attccggcag gattcagggg gtgatcatga gtcttttgaa tgaagttcag
     5701 aaattcattg aagcccatcc tggctgtact tccggagaca ttgcggatgc tttttacgtg
     5761 ggggcttaat gagtaataaa tattgccagg cgctggtgga actgcggaat aaaccagccc
     5821 atgaactgaa ggaagtggga gatcagtggc gcacgccgga taacattttc tggggaatta
     5881 acaccttgtt tggtccgttt gttctggatc tgttcactga cggtgataac gccaaatgtg
     5941 ccgcgtatta cacggcggaa gacaacgcgc tggcgcatga ctggtcagaa cgtcttgcgg
     6001 agcttaaagg tgctgccttt ggcaatcccc cgtacagccg cgccagtcag catgaggggc
     6061 aatacatcac cggcatgcgt tacatcatga agcatgccac tgccatgcgt gataaaggcg
     6121 ggcgctatgt tttcctgatc aaagctgcca ccagcgaagt gtggtggccg gaagatgcag
     6181 atcatattgc ttttattcgc gggcgtattg gttttgaact gcctgcctgg tttatcccga
     6241 aagacgaaaa gcaggtgcca acaggtgctt tcttcgctgg tgctattgct gttttcgaca
     6301 agacctggaa gggaccggca atcagctaca tcgggcgcga tgaacttgag gcatgtggtg
     6361 aggcatttct ggcgcaggtt cgccagcagg cggaaaaact ggtcagggag atggtggcat
     6421 gaagctaata ctgccttttc cgcccagcgt gaacacgtac tggcgacacc ccaacaaagg
     6481 ggcgtttgct ggtaagagcc tgataagcgc ggcggggcga aaattccaga gcgcggcgtg
     6541 tgcagcaata gttgagcagt tacgtcgtct gccgaaacca acgtcggcac ctgcttcagt
     6601 ggagatcgtg ttgtttcctc cggataaccg gatccgcgat ctggacaact ataacaaggc
     6661 gctgtttgac gccctgaccc acgcgggtgt gtgggaagac gacagccagg tgaaaagaat
     6721 gctggtggag tggggaccgg ttatcccgga agggaaggtc gagatcacta tcagtaagta
     6781 cgaaaaagcg agttgcaaat tagcaactcg gtaacggaat tgagcaacac cctaaatttg
     6841 ggtattacct cgttaaagat actgtattta tgaacagtgt atccttgata actattaaaa
     6901 atcgcagtaa gttcatcctg catcaacgaa aagggagtgc agtcccgctc gtggataaaa
     6961 atttgtggag aaaccaatga atcagttgct tgtaattgat ggcgtttctg tgcgccagta
     7021 cttcgaatct aactactgtc ttaacgacct tcagaaagct gctcttcttg ccgctggtga
     7081 gaatcgctcc tcccgttcgc tggaagttca cgagtttatg cgtcgtcctg aaacgaaggc
     7141 tcttgtggaa ttattggaag aagaaactac gggagattcc cgtagtattc ctgtcatcac
     7201 cattcagggg cgcaatggtg ggacgtatgt ctgtaaagag ctggtctatg catatgcaat
     7261 gtggatcagc ccggcattca gcttaaaagt gatacgtact tttgatgcgc ttcataattc
     7321 atcaccagaa gaaaccacat ccgacaaaat taaatccggg gtcattctgc ttgaatcagc
     7381 agcaaagact ctaaatctgt caaactcctc gaaacttggt gcataccaga aattatcaaa
     7441 ggtagctggt cttcctgaac ttatgccgat ctatgccatt gatgcacctg ctgatgcgcc
     7501 agatggttca agccgcccta cgctgtcgct gagtgcactg ctgaagcagt atggtatccg
     7561 cctgacggct aatcaggcat atcaccagat ggcgaagctg gggatcgttg aacaacgcga
     7621 acgatacagc cgtaccgcga ttaacaacat caaaaaattc tggtcgctga ccgcgaaagg
     7681 ctgcatgttc ggcaagaaca tcaccagtcc tgcaaatccg cgcgagacgc agccgcattt
     7741 cttcgaatcc cgatttcctg agctgttaaa gctgctcgat accgttcatt gaggtgactg
     7801 tgagagcact actgacccct gaaattgccc cgcgtatggg gatcgtattg ttcaggccag
     7861 gttcagagct gatgcccctg tttatgcagg ggcgtgtcct gctggagcct gagccggagc
     7921 gttattcatc tttcgccagt ggtgccgttc cggcggcatc acaaccgctg gcggatgatc
     7981 ctgccgttcg gtccgtgttc cgccatgagg cagtgatccg tcgtgctggt ggcgtggaat
     8041 gtcttgaaag ctggttactt cgtgaaaaag gctgccagtg gcctcattcc ggatggcaca
     8101 gcgagaacat gaccacaatg cgacacgctc cgggcgcaat ccgtctgtgc tggcactgcg
     8161 ataaccagct gcgcgatcag ttcacggaac ggctggaatc aatggcaacg gataactgtg
     8221 cccgctgggt gttgtctgtt gtgcgtcggg atctcggttt tgatgacagt cacgttgtga
     8281 caatgccgga actgtgctgg tggctgattc gtaatgacct ggcggatgcc ttaccggaaa
     8341 gtgcagcccg taaggtactg agattaccga agacggttgt gccgtctgtc acccgggaaa
     8401 gtgaccttgt gccttcggtt cccgccacca gtattatccg ggataaagcg aaaaaggtgc
     8461 tggcgctgaa agtggatccg gagtcgccgg agtcttttat gttacgccca aaacgtcgcc
     8521 gctgggttaa tgaaaagtac acgcgctggg ttaagacgca gccgtgcgca tgttgtggaa
     8581 agcctgctga tgatccccac cacctgatag gtcacggtca gggtggaatg gctacaaaag
     8641 cgcatgacct ctttgtgttg cctttgtgca gaaagcatca cgacgagctg catgcggata
     8701 ccgtggcatt tgaagagaag tatggctccc agctggagct gatatttcgt tttatcgatc
     8761 gcgcgctggc gattggtgtg ctgtcctgat tttgtggaga aagttgatgc gtgatattca
     8821 gatggttctc gaacgctggg gggcatgggc ggcaagtggt aacgccgggg tggactattc
     8881 tccgatagct gctggattta aaggcctttt accatccacc gctaaacctc gcccggcctg
     8941 cagcgatgat gacggcctta tcatcgaaaa ctgccttacg cgcctgaaga agaaaaaacc
     9001 ggacgagtat tcgctgctgg tagctcatta tctgctgcgc atatcaaaaa ggcagattgc
     9061 cagaacaaga aagaagagcg aaaaggcaat acgaattgag atgcagattg ctgaaggatt
     9121 tattgacgga tgtctgtcga tgctgggtgt aaggctggag atggacgact ggctgcccaa
     9181 aaaagtaaaa aatgattagc gcggtccgca aaaagtatgt cagtatgtta agagtggtta
     9241 ctacgccaca caacttaaac ccgccgcctg gcgggttttt tatgactgaa atcgcatcag
     9301 tacagtaaac gtgctggtgg tggtgaatac cggtctttca gcttgctggc tttttcgaca
     9361 agagttattg gtgtgtcacg ttaaccggaa aagggaaaaa gacatgctaa aacagcagga
     9421 tatgacagaa accgccagag tggtgtttaa tgaattaagt gccaccgaac cggcgacagt
     9481 cggggagatt gcgcagaata cttacctttc acgcgaacgc tgccagttaa tactgaccca
     9541 gctggttatg gcgggtctgg cagactatca gttcggttgt tacagacgcc ttccgcagtg
     9601 aaggattttt tatttgtggt aaatgggcgg ctggtgggtg tggtggttgt tgctttcccg
     9661 ttgctgaaaa agaaagcatc aggcgattag cagggtatca gttacccgtt gaaattttta
     9721 aatacctcac aattcaggcg gttgactgtt gtctggtttg cggggagttt gttaaaagaa
     9781 actggcatgg tgaatccccc tgtgcggagg ggcaatcagc aactggtgtt ttgtcaccga
     9841 cccttatcct ttctgtgcgg gttcaggtgc tgatactgaa ctcaccggga ggcacccggc
     9901 accatgcaat ggcacatagc gccactctcc agcccctctc cggaggggct tttctgtgcc
     9961 ggatacatca cagtttctgg aaccttgggt actacagtat cagtcagggt gctatatttt
    10021 cagatgtgat gaaagcctgt cagcaggcag ggcgtatcgg aaatgaccca gtagagaaaa
    10081 cgttgactca gataccggtg ctgagttacc gggaaaccgg catcacatga ccgctatcct
    10141 tccaggccca tccgctccgg tgggcctttt tactgcagaa aacaggttcc ccgttaaatg
    10201 ctatgttgct cacaattcag taagttgaca gttgcctgtc agactgggca tttgttaaaa
    10261 aaatttcgca tggtgaatcc ccctgagcgg aggggcgact ggtgacggta taatttctga
    10321 ttatcaaaac gagaatgacg cgggtttagt ggcaccgggc tgaactcacc gggaggcacc
    10381 cggcaccatg tgcatgatga tacagatacg cggctttagc ccctctccgg aggggctttc
    10441 ttgtgggcaa aaaaaagcct gagtgggttc gggcaacagc atgagatata catttttata
    10501 atcgaatgga ttttaaccag aattcataag gctgcgcaac tgcgtggcct ttttcgtatt
    10561 gcgggctgta gttttcctcc tgccattgtc ctgtaacttc cggacttcag cccgcccct
//
LOCUS       DAZFFE010000135        10576 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFFE010000135 DAZFFE010000000
VERSION     DAZFFE010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.135"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..65)
                     /locus_tag="ACHJ69_004156"
                     /pseudo
     CDS             complement(1..65)
                     /locus_tag="ACHJ69_004156"
                     /inference="COORDINATES: protein motif:HMM:NF013446.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            125..274
                     /locus_tag="ACHJ69_004157"
                     /pseudo
     CDS             125..274
                     /locus_tag="ACHJ69_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            301..405
                     /locus_tag="ACHJ69_004158"
                     /pseudo
     CDS             301..405
                     /locus_tag="ACHJ69_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            709..1002
                     /locus_tag="ACHJ69_004159"
     CDS             709..1002
                     /locus_tag="ACHJ69_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HFO5017021.1"
                     /translation="MVKLLTEPWGRKMHSDYYNMVFGEKLANILYEANSQFFYERNVI
                     EEAVNALFCEREIINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
     gene            complement(1340..1471)
                     /locus_tag="ACHJ69_004160"
                     /pseudo
     CDS             complement(1340..1471)
                     /locus_tag="ACHJ69_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1753..3111
                     /locus_tag="ACHJ69_004161"
     CDS             1753..3111
                     /locus_tag="ACHJ69_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFO5017022.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNHG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYQVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(3350..4735)
                     /locus_tag="ACHJ69_004162"
                     /pseudo
     CDS             complement(3350..4735)
                     /locus_tag="ACHJ69_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(4785..5132)
                     /gene="tnpB"
                     /locus_tag="ACHJ69_004163"
     CDS             complement(4785..5132)
                     /gene="tnpB"
                     /locus_tag="ACHJ69_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5017023.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(5129..5509)
                     /locus_tag="ACHJ69_004164"
     CDS             complement(5129..5509)
                     /locus_tag="ACHJ69_004164"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5017024.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(5864..6298)
                     /locus_tag="ACHJ69_004165"
     CDS             complement(5864..6298)
                     /locus_tag="ACHJ69_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO5017025.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDENYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(6286..6687)
                     /locus_tag="ACHJ69_004166"
     CDS             complement(6286..6687)
                     /locus_tag="ACHJ69_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO5017026.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(6947..7516)
                     /locus_tag="ACHJ69_004167"
     CDS             complement(6947..7516)
                     /locus_tag="ACHJ69_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO5017027.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            7715..7915
                     /locus_tag="ACHJ69_004168"
     CDS             7715..7915
                     /locus_tag="ACHJ69_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017028.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVCILCSW"
     gene            complement(8263..8472)
                     /gene="hha"
                     /locus_tag="ACHJ69_004169"
     CDS             complement(8263..8472)
                     /gene="hha"
                     /locus_tag="ACHJ69_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO5017029.1"
                     /translation="MEVKTKEDWLYQFRRCSSRETLEKVISHTRYKLTPAELAFNSAV
                     DHRLAELTMNKLYDRVPASVWKYVT"
     gene            9171..9377
                     /locus_tag="ACHJ69_004170"
     CDS             9171..9377
                     /locus_tag="ACHJ69_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5017030.1"
                     /translation="MNTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            9463..10035
                     /locus_tag="ACHJ69_004171"
     CDS             9463..10035
                     /locus_tag="ACHJ69_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017031.1"
                     /translation="MQKLENRCDLLLTQHQKWMASVTRFIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPATPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGESRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELESWAVARKREKRELELMIDEYLLYAC"
     gene            10244..10351
                     /locus_tag="ACHJ69_004172"
                     /pseudo
     CDS             10244..10351
                     /locus_tag="ACHJ69_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(10361..>10576)
                     /locus_tag="ACHJ69_004173"
     CDS             complement(10361..>10576)
                     /locus_tag="ACHJ69_004173"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017032.1"
                     /translation="INVLVKIARMTVFADSVQWKRYYIIYLSNKFNTNFRRCVGELMT
                     NFQRGMEKCMESHKEKIVIVQLTFISG"
BASE COUNT         2619 a         2739 c         2583 g         2635 t
ORIGIN      
        1 ctcccgaact caaagctatc atacagataa ttactggtta accctatata gggcatttta
       61 aagtgttgtg atcttaccca gcaatagtgg acacgcggct aagtgagtaa actctcagtc
      121 agaggtgact cacaagacaa aacagtttca accagtaaaa aaacgtaaac accattcgca
      181 tgaatttcgt agttaagatc tgaagcttgc agaatgcatc ggtgttactg ccggagtccg
      241 tgaacttagt ctgcatgaat caccacagct ttacatctgt cgcagtaaac agcaaaatca
      301 gcaaacttac ttaattatat cgagtgcgat tacaatcgct ggcgatggta cagttggtgt
      361 ggcggtctca gtccggaaca atttgaaaat cagaatctca cttaaaacgg catccatttt
      421 acaaaagatg gccgtctgtg tatttgtaat gaataccttc tacatgttta gagtgcataa
      481 tccacgacaa ggcgatatcc taaaaattag ctagcaggca actttgatgg catagataat
      541 gtttttagta aaaaagagtt gactgaatat aatacagcct cttcctacat aagaaggaat
      601 gtgctcacaa aaaaaaacca ttagtctgct tcctgttctt acaataatca gtattatgta
      661 ttttatttat atagcaaaat atgcttatta cacctgtcat aattaggcat ggtaaaatta
      721 ttgactgaac cctgggggag aaagatgcac agtgattatt ataatatggt ttttggtgaa
      781 aaattggcca atattttata tgaagctaac tctcaatttt tttacgagag aaatgtcata
      841 gaagaggcag tcaacgcttt attctgtgag cgtgaaataa taaacaacaa gaacataata
      901 aagaagctga tgttcttttt gtcagatgtt aatcacacca agaaagatgt tgttcagagc
      961 gcacttaata ttatcattga cattacatca ggtgatattt gagattttac ataaaagata
     1021 atctctcaag gagtcttatc tgaaaacgaa atatatagtc cctctcgttt tgtaatatcg
     1081 attggctttg ttgaatcggt tacgtacccc ggagccggtc atattttcag gtaatacaca
     1141 cgctttcaga catttcggct ttcgtcattt tggtatcatg ggcatagctt ctgctacctg
     1201 agcatcataa tcacgcagct tcagtgaagc agtgaccacc tgtttaaccc ggtgtatcgt
     1261 tatgatctta tccagcaata atggacacgc ggctaagtga accgccccgg gtttcctgga
     1321 gagtgtttta tctgtgaact caggctgcca gatcatcgtt tccgatggaa gcataataag
     1381 ctttttctgc ttctgccggt gggatatggc cctgcctttc cagcaatcgt cgattgttat
     1441 accagtccac ccacgtgagt gtggtcagct catcatactg acacgtgcat tacccctacc
     1501 gcgataatgc aaccgtacaa tatagggcgt ttcctaaacg cagccctcat cactccaatc
     1561 caacaagaca cagcattttt gatcacgaag aaagacaaat aatttccgta aatgtttccg
     1621 ttgttacaaa taacaacagt gttttacatt tctttggcct caggcccgcg catgttgcca
     1681 ttcgatgtta ttttcctgac tcgtttcttc tttatggtgt agtgtgatct tcactttatg
     1741 gatgctaaca atatgaaaag aaaaattatt cccgtgctaa tcggttgtac gctctctttc
     1801 tctgccctgg cggcacaacc tacggctgag cgctatgtcg tcagttttcc agaaggcacg
     1861 cacgtgaact acgccggcgc atttgccagt gcattcccga acggccttcc ggttgggata
     1921 ggttctggcc tgctgttcac cggtaaacag ggcgatgccc tgacattcgc aaccattacc
     1981 gatcgcggtc cgaacgcgga ttcaccgaaa gaggggaaaa acgaaactaa aattttcgtt
     2041 acgccagatt tcgcgccgct gctgatgacg attcgtgtac aaaacggcaa agcggaggcc
     2101 atcgatcccc gtcctctgca tgacgataaa ggtgcgatca acggtctgcc gctggcaagc
     2161 gacgtgattg gctccaccaa tgaagtggcc ttcagcgaca cgttacatag actgaagggg
     2221 gataatcacg ggctggatac ggaagggatc actccggatg gtaaaggagg ttactggctg
     2281 tgcgatgagt atggcccgtt cctgattaac atcgacagta aagggaaaat cctggcaatc
     2341 cacggtccgc aggcggcgga aggggaaaaa gcgattgcgg gcggtttgcc aaatatcctc
     2401 aaatggcgtc aggcgaaccg cggctttgaa ggcctcaccc gtatgccgga cgggcgtatt
     2461 attgtcgccg tgcaaagtac gctggatatc gacgctaaga gcaagaaaaa ggcgctgttt
     2521 acgcgtctgg tgagcttcga tccggcaagc ggaaaaaccg cgatgtacgg ttatcctatc
     2581 gacagcgcgg cctacagcaa aaacagcgac gctaaaatcg gagacatcgt ggcgctcgat
     2641 aaccagcaca tcctgctgat cgaacaggga agagataaaa acaacagaat gcgtaacctg
     2701 atttacgagg tcgatctcaa taaggcgagc gacctgtcag gcttcgacaa gccgggtgag
     2761 tatccggagt ttgatgatga gaaaaccctg tcacagcgcg gcattaccct tgcgcagaaa
     2821 acgcaggttg ttgatctgcg ctccttgggc tggcagcagg agaaggctga agggttggcg
     2881 ctgatcgaca gcaaaacgct ggcggtggcg aacgataacg actttggcgt gaaggtggcg
     2941 atgcaacatc cggtcgaggg caagacattc aaggattatc aggtgaacgc ggaaggaaag
     3001 ctgacgcttg acgacaagca ggttgaaacg accctcagag tgaagccact ggagaaaccg
     3061 gagtctgaca gtgaactgtg gattgtgacg ctgccggaag cgttgaaata agtttataag
     3121 gtacggacag cagtatcgct tagtttttca aattcatgta tgtgaacaat attggggttc
     3181 cggcgtagac cctcaatgaa agcgtcacca cgtgaaaaca tgcctgcacg gtttacacgt
     3241 catcccatgg tggcagccgg ttaactttcc tgctacacca gtggaaaaag gccataagca
     3301 gtccgaacaa aagtaagcgt acagcgaggg ccgtattgac ggggatgtgt tattcagctg
     3361 gcagtgctat gcgccacgga agcagttcgc tgacccggtt gaccggccag tctgctatga
     3421 cgccaagcac atggcgaagg tagctttctg gatccacgtc attcagtttg cacgtcccga
     3481 tcaggctgta cagtagcgct ccccgctcac caccatggcc agagccgaag aacagggtct
     3541 tcatcttttc acgcaaccag ctttccaggg atttcaacag cggtttcgtt tttcgctgac
     3601 gttcagcaag ccgctgctct gccggcattc cccttatatc cgcctctatg gcgtacaact
     3661 gaccgatctg ctccagggct tcttccgtca gtgctgacgg gatgcggacg tgcacatcgt
     3721 ggatctttca gcgggcatga gcccagcagg cagcttccgt tatcccacca ttgcgataca
     3781 gctcgttgaa cccggcgtac gcatccgctt gcagcacacc gctgaagcaa gcaagatgag
     3841 tctgcggatg gatgcctttt ctgtccgggc tgtaagcgaa cctcactgca ggtgccaacg
     3901 ctgacccggc attgcggtca tcacgaacat acgcccacaa ccgcccggtc ttcgtcttct
     3961 tattacccgg cagcagtacc tggaccgggg tatcatcggc atggagtttg ccgtcagtca
     4021 tgacatagcc atgaagcgcc tcttccagcg gagacagcag ccggcagcat gcatccaccc
     4081 agcccgacag cagtgaacgg ctcagctcca caccttgccg gccgtatatt tctgactggc
     4141 gatacagcgg ggtgtgctct gcatacttcg aggtcagcac gcgggccagc agccccggtc
     4201 cggcgatacc ccgctcgatg ggccgcgaag gtgcaggtgc ctgcacgatg gtatcgcact
     4261 gagtacaggc atgtttttcc cgtaccgtcc ggataacccg gaaggcgcta cgcatcaact
     4321 ccagctgttc ggcggtatcc tcgcccagat agctcagtga accgccgcag ttcgggcagc
     4381 acggcgccgc aggcaacagt cgcttttcgt cacggggtag tgattcaggg aacggcttac
     4441 gggtgcgggt ctgacgcaac ggacgctgta ctgccgggtc atacacccta ccagtcagcg
     4501 tatcgctctc tttctgaagc cggttcagat cggcttccat ttgtgcgata cggcgggaga
     4561 ctttttcgga acgactgccg aagttcatcc ggcggagttt atccagctgc gcctgcagat
     4621 ggtctatttc gcgctcccgg ttgctcagct tttccagcag ggcgtggatc agcgcttcct
     4681 gttcggccag gcgctgtttc agcaggaaga tgtcgtcaga agagatgtcg ttcataagcc
     4741 cgtattttac cgggcttatt ctgtggcaac caggataaag agatttacag catggtcagg
     4801 gaggtcagca gccgcttggg ctgtcgccag tcgatacctt ccagcagcat cgccagctgc
     4861 gcctgcgtaa ggaacacttt gccatcacgg gctgacggcc aggcgaagcg cccacgctcc
     4921 agccgtttgg tcaggaggca cagtccgtca ccggtggacc acagcagttt aacctgactg
     4981 ccgctgcggc cccggaaaat gaaaatatgg ccggacatgg gatcgtcttt cagcgccgtt
     5041 tgtactttcg cagccaggcc gttgaagcta tttctcatat cggtgatacc ggcaaccagc
     5101 caaattttgg tcccggaagg taacgggatc atcgcttcag ttcctgtatc agcagagtca
     5161 ggagcttttc gctgacattg ccattgaagc ggagcgtccc gtgccggaac gttacctcac
     5221 agctgatact gagggtttcc gggtcctctg cgagcgattc tggctgttcg gcagctgcat
     5281 cgagagtcac aggaagtagc tgggggctct ctgaagaagg taatagcagc tttccctcgc
     5341 gccattgttg tcgccatttg aacaacagat tggcgttaat gccattttca agagcaagtt
     5401 ttgagatgga tatcccgggt tcacaggagg cagcaacgag cagctgttta aattcgggag
     5461 gataattagg gcagcctttt cgcctgccgg gagtcacatt tttctgcata tctgacactt
     5521 tggttcccac tacttatttg gtggacacca ctttgtctaa ttcgtcagat tctgaccaga
     5581 cggttcaggc tgtacgctta ccactatttt atccctgttg aatttggtta agccggtgca
     5641 gacgcgccgg gaaacgcaca cctgaaccgg cacaataaaa tacaatacaa ccccctgtta
     5701 ctggagatac gaatggaatg acgaggcagc ctgtcagtca cagggggctg ttggcataaa
     5761 atcaacggaa aggccaccaa caccggaatt aagctccaca gtccccgcac tggcaatcca
     5821 ttcccgggaa aggcatcacc gcaaactgct ccggaggcaa ccattacagc ctctgtaacg
     5881 gattatcagc cctggcatct tccgacggca cctcctgccg cccccacgcc ctgctgtata
     5941 ccccggtacc gcagcgtggg cagcactttt ccccctcata attttcgtca cacatcacac
     6001 aaaaccactt tctcaccttc accgtataat ccggaaccgt catgccctgc agatgcaaac
     6061 gacgggcctc tttctctgcc gggtcaagcc aggtacatcg cagcaggatt ttttcagcga
     6121 cagtcatctg gtcatgttca aaccacggtg gttgtccgtc attgccccgc tgaagtgtta
     6181 acgggcatcc acaggacaca cagcacaaaa cgtcctgtcg gttgtgcttc gcagcttctt
     6241 tcgccgtaac aaaatggcct ttgctgtcat tcgccatata acagttcaga attcgcatat
     6301 ttcctcctga gttgtccggg gaatactgaa tcctccggtc tggcagtgtg tgcagtactg
     6361 ctccccataa taatcgtggt gacattgtct gcagtgccag ctggctttac gcaccacggg
     6421 taaggcatcc ggtacgaatt gctgcagacg tttaatcaac tgtatttctc tgcgctccgg
     6481 cctgacataa gggcactgtt gaccgtgctc tgtcagcctg tcgtcagtgt gttcaaacca
     6541 gggaagttca gtgtcgtatt gcggatggta tctgagtgca ctgccacaca agtggcaggt
     6601 gtagttagca taaggtgcgg cctgtgcagt acgggcgccc gtcagacgtc cgttgccatc
     6661 aagagcgata aaggattttg cgtacatagt catatacctc agccgccaga cgacacgcag
     6721 gcaacagccg tcccggtacg ggcagcgtgg tcagggtgtg aatggcggtt cagttaaaat
     6781 ctggactgga gtttgggtaa agcaggctgg ttgctgttac agcactgtgg ttgtcacaca
     6841 atggaacact gacgtaaaaa acagtcagtg ggcgagtgga tacatcgttg ccgccagaca
     6901 acaacccata cttttggttt gccggatatt ggcgcgccag tcgtgctcaa ctccggctac
     6961 agccaaaatt gcgctcaccg gtgcagtgag ctttggtatg ctccttcgcc agatagtcag
     7021 cacgttccag cacttgctga aagccggtgt catcattacg cgccagccac accgccggcc
     7081 aggccggaaa atgcaccagc gtggcatggc acccgacatc cactcccagg gcactgcacc
     7141 acgcctgttt aatcatcccg gcgagagact ccggagcgcg gtaatcaccg gcacgacacc
     7201 aggtatcccg gttgaccagc agcatcaggt gatagtgttt tttacctttg cactccccaa
     7261 actcccgggc ccagacgtaa tgcagggttg tgggatgcac gcgtttacct tcccgatgct
     7321 tacgtttctg gtaagcgtcg attcgggctt tcagggcatt gatgaagcgg gatatcacag
     7381 ctgcgtccgt tgctgccggc acatccggga gacgcagatc aacccgaagt gccgtcaggc
     7441 ggggatgaac atccagtgcg tgccgcaccg tctcacgaat acgttgctgc cagaaggggt
     7501 tgtatttgta ggtcatggtt aaatctccgt atggttcata cggaatagcc acgtcgtaaa
     7561 aaatacgcag agtccctgaa gtgacctccg gcaggacact gtctcaggca ctctgtgata
     7621 acccgaacgg tcgtctccgg actgacggtt gaattttcag cgttgttttt taatgtaaaa
     7681 aaccggttac gggtaaggct gtgaggaggg agtgatggtg atacgcaaaa agaagtgcag
     7741 ggactgtgga aacgcgatta ctcataatac agtgtgttgc ccgtattgtg gttctgtcga
     7801 ccccttcggc tactaccgga atacagaccg tatcgttacc atactgctgg cgctgattat
     7861 tgtggtcctg ctgactactg tcagcgtcag tgtgtgtata ctgtgtagct ggtgagataa
     7921 cggttcatgt atcccctgag ctgacaatgt accgtgtata ttaagcgtaa ccgacaggtt
     7981 ctccttcata ccatattaaa ttttccaggt attgccgctg ccttatttcg tcccatacag
     8041 gaaaactcat ctgggtttct gacaaatacg cgaaactcac agcccctcaa tatcaaccac
     8101 acgttgtaag ccggtaatac ctgaaaatca gcctattacc gcccacgcct ccggcaccat
     8161 tcccttttga tttcgaattc tttgttgccg ttatactgct taatgttccc gatcttccgg
     8221 tgcacagata acaccggagt catcgtagcg cggattcaag attcaggtga catatttcca
     8281 gacggaagcc ggcacgcgat cgtaaagttt gttcattgtc agttctgcca gccggtgatc
     8341 gaccgcagag ttaaaggcca gttccgcagg ggtaagttta taacgtgtgt gggaaatcac
     8401 tttttccagc gtctcccggg atgaacaacg acggaactga tacagccagt cttctttggt
     8461 tttaacttcc attagcctct catcacttta tgctgcaatt aacgtgatac tgccagcata
     8521 ccgcacctga ctggcattct ggtacccacc ccaaactcat tactcccgca ccaccactta
     8581 accggtctct cagactgtca ctcaccaggt cggatacgtt taccgctcag acggatgctt
     8641 aatgcactct gttcacgtgt gttgcaggta cgttttatat acgcaggtta tacgccagct
     8701 atcagggata gccctgtgag tacaacgtaa tctgttgcta ctgcgttctc atacccactg
     8761 atcacactca cagtgtttat ttcacagata tgaactcatg tgtttgatct ctcttatata
     8821 tttataatac caatcagaca gtcttatccc atcggtgata cctgtctctg cccacaaggc
     8881 acgagctgcc ccaccgtaac cgccccgtaa ataactctgc aattataaga acgacggttc
     8941 ttcccctcat cctcccgaaa atcatccctt ctctgtcgtg cgtacgcgcg agaaacatcg
     9001 tttcgcggta gccaccgggt atcccagcat catgctccct gtttatctcc cgacaggaat
     9061 attcccctgc tcatttttta cgtttctccc cggttgttat gccagtgaag gtggtgcgtt
     9121 tttcatcacc aacgccggtt gagattaaca acatccggag gaacattttc atgaataccc
     9181 cagtttcgct gatggatgac cagatggtcg acatggcgtt tatcactcaa ctgaccggct
     9241 taaccgataa gtggttttac aagctcatca aggatggtgc ctttccggcc cccatcaaac
     9301 tcggccgcag ctcccgatgg ctgaaaagtg aagtggaagc ctggttgcag gcgcgtattg
     9361 cacaatcccg tccgtaattc ctgaccatta cccgttcaac cgcagcgaat acactatccg
     9421 gcctccggag gcattttgct gcgtgctaaa ggagagaata ttatgcaaaa acttgaaaac
     9481 cggtgtgatc tgctgctcac ccagcatcag aaatggatgg caagtgtaac ccggtttatt
     9541 gtcgctcacg gaatgggctc tccgcatctt catggttatc atcgcctgac cctggcccac
     9601 ttttttctgc cggaaaaagg caccatcatc tcggtggcac cacagggact ctatcaggta
     9661 gtcaacccgg caacgccacc gttcattcct gccattcagg aagggttaat gaccagcatt
     9721 cagactcatg aaatcatgtt acttacacat tttaacctgg gtggtgtttt actcagtgaa
     9781 ctacatcgtc tgggagaaag ccgactggca aacaggctga acagtctgct ccggcgtttt
     9841 gacgatcgcg atctctacca caccctgata tggctgtgct ggtatgatct gatgtgcgcc
     9901 cactccatgc agccctggac tgaagagtta aagcataaat cacacgcaga acttgaaagc
     9961 tgggctgtgg cgcgcaaacg ggaaaagcgc gagcttgaac tgatgattga cgagtacctg
    10021 ttatacgcct gttgattcat aatcactccc gggcggtacg tagtcagtga taccggaatt
    10081 tccggagagg gtcgcaggta tattgtttct gtgcaattca tggttattca cgttggatgt
    10141 agtgattgct gcatccaatg ctatcggaaa gagattaacc agcaaatggt tcctcaaatc
    10201 gtattgatat ttacccggcg cattgagcac caccatctgc ccccgttccc acatcaaacg
    10261 gctggaccgg aaaacgattt gcttttcatg ctccgtggaa attcacgaaa aagttattgg
    10321 gtcattcatc gaaaaaaaca tattctgctg actggagtca tcacccagaa ataaacgtca
    10381 actgaacaat aacgattttt tctttgtggg attccataca cttttccatc cccctctgaa
    10441 aattagtcat caactcacca acacatcttc tgaaatttgt attaaactta ttgcttaaat
    10501 agatgatata atatctcttc cattgcacgg aatcagcaaa aactgtcatt ctggcaatct
    10561 taacaagaac atttat
//
LOCUS       DAZFFE010000136        10474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFFE010000136 DAZFFE010000000
VERSION     DAZFFE010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.136"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..566
                     /locus_tag="ACHJ69_004174"
     CDS             <1..566
                     /locus_tag="ACHJ69_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5017033.1"
                     /translation="DIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQE
                     DIELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSG
                     EVIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNS
                     KKEVADELGIGIATLYRKIKKYELLNT"
     gene            complement(606..1085)
                     /gene="xdhC"
                     /locus_tag="ACHJ69_004175"
     CDS             complement(606..1085)
                     /gene="xdhC"
                     /locus_tag="ACHJ69_004175"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO5017034.1"
                     /translation="MNHSETITIECVINGMPFQLHAAPGMPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLFLAAWAEGKEIRTLEGEAKGGKLSHVQLAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(1082..1960)
                     /gene="xdhB"
                     /locus_tag="ACHJ69_004176"
     CDS             complement(1082..1960)
                     /gene="xdhB"
                     /locus_tag="ACHJ69_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO5017035.1"
                     /translation="MFDFASYHRATTLADAITLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITQAEDGALRIGSATTFTQLIEDPVIQRNLPALCAAAASIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRYSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1971..4248)
                     /gene="xdhA"
                     /locus_tag="ACHJ69_004177"
                     /pseudo
     CDS             complement(1971..4248)
                     /gene="xdhA"
                     /locus_tag="ACHJ69_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            4662..5417
                     /gene="actS"
                     /locus_tag="ACHJ69_004178"
     CDS             4662..5417
                     /gene="actS"
                     /locus_tag="ACHJ69_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO5017036.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            5496..5569
                     /locus_tag="ACHJ69_004179"
     tRNA            5496..5569
                     /locus_tag="ACHJ69_004179"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5528..5530,aa:Gly,seq:ccc)
     gene            complement(5886..7115)
                     /gene="yqeG"
                     /locus_tag="ACHJ69_004180"
     CDS             complement(5886..7115)
                     /gene="yqeG"
                     /locus_tag="ACHJ69_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5017037.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWKSIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            7370..8551
                     /gene="yqeF"
                     /locus_tag="ACHJ69_004181"
     CDS             7370..8551
                     /gene="yqeF"
                     /locus_tag="ACHJ69_004181"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO5017038.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVITQSNGQTLVVDTDEQ
                     PRTDTSAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            8838..9674
                     /gene="kduI"
                     /locus_tag="ACHJ69_004182"
     CDS             8838..9674
                     /gene="kduI"
                     /locus_tag="ACHJ69_004182"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO5017039.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            9704..10465
                     /gene="kduD"
                     /locus_tag="ACHJ69_004183"
     CDS             9704..10465
                     /gene="kduD"
                     /locus_tag="ACHJ69_004183"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO5017040.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSETDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
BASE COUNT         2653 a         2459 c         2676 g         2686 t
ORIGIN      
        1 tcgacatccg catcatttct gcaactaatc agaatttggc ccagttcatt gccgaaggta
       61 aattccgcga agatctcttc taccgactta atgttatccc gataactctt ccaccgctgc
      121 gtgaacgtca ggaagatatt gaattactgg tgcattactt tttacatctg catacccgtc
      181 gtctgggatc ggtttatcct ggcattgctc ctgatgtcgt cgaaatattg cgtaagcatc
      241 gttggcccgg aaacctgcgc gagttaagca atttgatgga atatctggtt aacgttgttc
      301 cttcaggtga agttatcgac agcacgctat tgccgccaaa tctgctgaat aatggcacaa
      361 cggagcaaag tgatgtaaca gaggtcagtg aggcgcacct gtcactcgat gatgcgggcg
      421 gcacggcgct ggaggagatg gaaaagcaaa tgatccgcga ggcgctttca cgtcataaca
      481 gcaagaagga agttgctgat gaactgggca tcggcattgc tacgctctat cgcaagatta
      541 agaaatatga gttgttaaac acataacctc tgagaatccg gcctgaattc aggccggata
      601 tccttttact tcgttttctc gcaatccaga actgtattta caatcatctg ataccccgtg
      661 cagcgacaaa gatttcccgc cagtccacga cgaatttccg taatggttaa tggtttttcg
      721 cgtggttttg ccagcatcgc cgtggtagcc ataatcaggc ccggcgtaca aaacccgcat
      781 tgcactgcgc cggatttcgc ataagccagt tggacatgag aaagtttacc gcctttcgct
      841 tcaccttcca gcgtgcggat ctcttttcct tcagcccagg ccgcaaggaa taagcaactg
      901 tctatcgcag tgccgtcgac cagcaccgta caggcaccgc attcgcctac gcagcaacct
      961 tgtttgacac taagaagccc ttgttctcgg agtagttccg aaagcggcat tcctggcgcg
     1021 gcgtgaagct gaaaaggcat cccgttaatg acgcattcga tggtaattgt ttcgctgtga
     1081 ttcattgcaa ttttcccccc gccgcggcga cggcttcgct aatcactttt ttggtcatcg
     1141 tctggatgag atgcagacga aactctttac tggcccgcca tgaataacgc ggggcgacat
     1201 cttgcaggac tgattcgctg atggcttcca gcgtttgcag gtttaatggc gcattttgtg
     1261 cagtctgttc ggcatgttgg cagcgaatcg gcgttggcgc ggcaacacca aatgccaggc
     1321 gtaattcgct gaaattgccg ttatccagtc ggcaatgtgc agcgcagcca attgttgaaa
     1381 tatccattgc gtcgcgcatg gcatatttaa aatgcgcgct gcccgcgtgt tctttcggct
     1441 gtggcggaaa atgaaaggcg acaaggattt cgtcatgctc aagagacact ttgcccggcc
     1501 cggtgtgaaa gccattaatc gggacgaaac gaacaccgcg cggggagtgg atctccagtt
     1561 tcgcgtcata aattagcgtt ggcgtggcag aatctgcgct ggtggcaccg ttgcaaatat
     1621 ttccgccgta ggtggcgaca ttacggatct gcggcccggc gattgatgca gccgcagcac
     1681 ataacgccgg gagattgcgt tggattacgg gatcttcaat gagctgagta aatgttgtcg
     1741 cggagccgat tcgcagcgcg ccatcttccg cctgtgttat tccccgcagc tctgccagat
     1801 tgtggatatc aacaatatgg cgatagcggt cattgtgatg gtgaagctgt atcagtacgt
     1861 cagtgccacc ggcaagcaat ttggcctgcg gattgtcagc cagcagggtg atggcatcgg
     1921 caagggtggt tgcgcgatgg taagaagcaa aatcaaacat gatgttatcc tcaaatcaat
     1981 cctgccagat ggaattcttc atataatcgt tttggcgtta gcggcagtgt attgattgca
     2041 acaccggtag ccatcttcac cgcgttacga atagcagcgg ctacaggaat tatgggtggc
     2101 tcacccagtg acttatgtcc gtatgcggat tgcggctcat tgatttcgac gaacgcgctt
     2161 tccagttgtg gtagatccgg catggtcggc attttgtaat ccagcagatt ggggttacgg
     2221 accacgccgc ttttcgcatc gatgatcatc tcttcaaata gcgcccagcc aatgcccatc
     2281 cccattccgc cgtgtacctg accttctgcc agccgaatca tgaacgttga ggatgcggtt
     2341 gatggttact ttacacagtg caatatcgac cgtcagatca acaaaggtac agccaaacgc
     2401 cggtgggtta gtggtggttt tgatggagct ttcggcagag agctgcccgc cgcgttcagg
     2461 gtggtagaaa gcgtccatcg ccaaatcttt taacgacatt aacggctctt ccggtcgttc
     2521 aaccagcacg atatggcctt ttatcagggt cagattcatc gctgactgat gtagcatgac
     2581 tgcggcgtga gcgatgattt tctcttttaa taacagtgcc gcactgcgca gcgcaggcgc
     2641 ggcaacatag ctctgacgtg aggcaaatgc gccggggtcg aatggtgtaa cgtcggtgtc
     2701 ttgtgtcgaa attacgcgga catcgctgac cggaactccc acggtttctg ccaccatttg
     2761 cgagaacacg gtgtcggcac cctggccgat ttccgtcgcg ccgctttgca cgttgatggt
     2821 tccatcctga ttcatcaaca ggcgcgcgcc tgctatttct acgccgacag gccaggtgtt
     2881 agaggtgtag ctaaaacagg cgacgccaac gccacgacgt aaattgcctt gctggttctg
     2941 gcactctgca cggcgttttt cccattcaaa gattttccgg cctttttcaa gacactccgg
     3001 caaccctgcg ctgtaaatac gttttcccgt gagcggatta gcatctcctt cgcgggcggc
     3061 gttgcgtaaa cgaatttcaa caggatcaat acctaacgct gtcgcggcat catcaagcat
     3121 agactcaacg gcaaatacga cttgtggcgc gccataacca cgcatcgcac cagccgaggg
     3181 gaggttggta tagcaggtct ttgaactgta agcgtaggca caacgaggat aaaggtaagc
     3241 gactttattc cccccagcag aagcaatgga gtgcccgtga gatgcataag cgccggtgtt
     3301 agacagaaca tccagactat aacctttcaa tgttccgtcg cggttcacgc ccatttgccc
     3361 gtcaatagta aaagcgtggc gggtacgggt tgcgaggaaa cactcttcac ggctaaggga
     3421 aactttcacc ggaatgccgc caagtttgct ggtcaggaat gccgccattg gctcttccag
     3481 tacatcctgt ttattaccaa aaccgccacc gataaacggt ttgatgactc gtacgcatga
     3541 ccagggaata tccagcgcct gaccaaccac gcggcgaaca atgtgcggga tctgggtgct
     3601 ggaaacgatg gtaattcgcg agtcatcctc catccatgcc agcgatgtca cgctttccat
     3661 atgacaatgt tgaataacgg gagtctgata gtgcccctgt acctggtagt cggcggcatc
     3721 gattgtttgt tggacattac ccgtcgacat cgtgctttgt ttcagtaaat tgccaccgtt
     3781 atggattggt gcagcgtctt ctgccagcgc cgcttctggc gaggtgataa cgggtaattc
     3841 ttgccactca atgctgacca attgcgccgc tttttctgcc gtgagttcat cgcgggccac
     3901 gacgatggca acggcgtcgc catgatgacg aacatggcga gttagcagtg cgcgatcggc
     3961 ggtatcgcgc ttgttttcgt caagtgtcca ggcatgccct gccgtggcga atgggatttc
     4021 tggcacatct tcccaggtaa aaatcgccag gacgcccggc aaactcctgg cttgttcatc
     4081 attaatactt acggcataac catgtgcgat aggactacgt acatatttcg cgtaacacat
     4141 gcccgccata acataatcgt cagtatatcg tgcccgcccg gtgaccttag caatggcatc
     4201 gacgcgcatg catgattcac ccgtagcggt tgcttcccgc gcttccatag aaatcccctc
     4261 ggtttgaaca tttggttctt atggcaaaga tttagtctta gcaggcaaga attacgccag
     4321 aaacgactat atgcgtgagg ggaggaagga agtgtgatga cgttaaaaaa acacagatat
     4381 gatacgaaac aggactctga atagggacag tgatacgtat caatatgagt ggcgataaaa
     4441 tcaatgtgat aaattaggat tattacgcat caaggtggga aatagggtaa aagtcaggaa
     4501 cttcgatacc actacagcaa atattttgct gatgatactt ccctttgcca aatgaaatta
     4561 acctctttct ctattacatc gcttttccag aagcaagaga ttgggtatcc agaagccggc
     4621 tgctatgata tggcgtcttg ttttttaagc gaggaaagat tttgagtgcg ggacgcctga
     4681 ataaaaaatc tctgggtatc gtgatgttgt tatcggttgg actgcttttg gcgggctgtt
     4741 cgggtagcaa atcatccgat acaggaacgt attccggctc cgtttacacc gtgaaacggg
     4801 gggatacgct atatcgtatt tcgcgcacca cgggaaccag cgtaaaagaa ctggcgcgac
     4861 tgaacggcat ttccccccct tacaccattg aagtaggtca gaaactaaaa ctgggtgggg
     4921 cgaaaagtag cagtagtaca cgtaaatcaa ccgccaaatc aacgaccaaa accgcatcgg
     4981 ttacaccgtc atcagcggta ccgaaatctt cctggccgcc agtagggcaa cgttgttggt
     5041 tatggccaac gacagggaaa gttatcatgc cgtattcgac agcagatggc ggcaataaag
     5101 ggattgatat ctcagctcca cggggtacac ctatttacgc cgcgggtgca ggaaaggtgg
     5161 tgtatgtggg caaccagctg cgtggctacg gtaatctcat catgattaaa cacagtgaag
     5221 attacattac ggcttacgct cataatgaca cgatgctggt aaataatggg caaagcgtga
     5281 aagctgggca aaaaatcgcc actatgggga gcactgatgc ggcatctgtt cgcctgcatt
     5341 tccagattcg ttaccgtgca acggcaattg atccgctacg ttacttgccg cctcagggca
     5401 gcaagccaaa atgctgatgg cgaattaatc agcagtcagc agcgtggtac ttgctaagga
     5461 gagcgtaagg tttataatgc cttacgcatc tcgaagcggg cgtagttcaa tggtagaacg
     5521 agagcttccc aagctctata cgagggttcg attcccttcg cccgctccaa tttatcttaa
     5581 catcatctac ctgccatttt tgttgtatct gattttctat attatcttaa ttcaagcgct
     5641 tcagactttt cgcaatcgcc gtaatacttc cccatttttt tgtaaataaa aagcgtttac
     5701 gcaatgcaat cgtcatgtat cttaaatagt cttatttaag atgttcagaa ctctttgtgt
     5761 gtttaactag ctgataatta tataatatta cgagagaagt gatagggcgg aataataaca
     5821 aatgaacatt atgaaaactg ggatggttca aaattgaagc aattttgaac catccacaac
     5881 ggatattagc taaagaacat taccgatacg cacaggatac ccacgatcag cgtaatcaga
     5941 tttccgatgg aacgccaggg tttaagcgcc gggatgagat acgttgacag cgtaggcatg
     6001 atgaaaagta tcatggcaat gagcgggccg ctgatcgcgt aaatcatcga aatcgcgttc
     6061 gggttaatgc aacaaacaat gaaggtaatc agcgatacca acataattga tagtgcgcgg
     6121 ttaaatgcac gacttttctt tacaccaacc tgctgtaatg ttgttttgac gacctctgtg
     6181 gcaccttcaa taacgccaaa gtacgtaccc aggaatgatt tcgacatcgc aactactgcg
     6241 acaataatcc cggaaattga taaccatgct ggggcgttcg gcagcataga aagcgccgat
     6301 aaaatggtaa ccccttcttc tttagccgct tcaatatacg aaggtggaat agaaagcagg
     6361 cagctaaaga caaagaacaa tacacttatg cagatgatga gataagcgac tttcataatt
     6421 tttttgcatt tatccatagc atgttcgcca tatttttcac gtctgtctat ggcaaacgta
     6481 gaaataatgg gcgtatggct aaaagcgaaa accatcacgg gaatcgattt ccatatctgg
     6541 tgaagggtat tctgattgaa ctctacctgg gttgttaata gatcaggttg ccaactaccg
     6601 accaggtaaa tagaaagaaa taagaaatag gcaatcaatg ggaataccaa aaatcccatt
     6661 acccgaatgg ttgcatggcg tcccatcaga aaaatgagat tcaggattaa tacaaccccc
     6721 agactcacca acatacggat gcgaagatca ataaccatat gctttgccag ctgttccgta
     6781 agtgagttgg taattgccac tgcatatatc aacacgacga caaaaaaggc gatgaagtac
     6841 agcgtggtaa tcagattacc aatcttcttg ccatagtagt gtgttaccgc gcccgttatc
     6901 ccttcacctg ctgatgtttt cgatgagagg atgaactggc ataaggcttt atgtggccaa
     6961 tatgttaaag gccaggcgac cagagcagta ataaacagga ccacagcccc cgccgaacct
     7021 aactgaatag gaaggaagag cgtgcctgca ccaacggctg tgccgtagag cgcgaaactc
     7081 cacagagttt cttcttttga ccaaatatta gacattatct gaacgtatca tctacaaatt
     7141 aaacaaaatg gagggcaatt taccataaac caggttaagt atgggagatt aacgccctat
     7201 ttacctctct attggtgagt tagtggttgc aaaccttacg tgccagttat gtctctgact
     7261 actactcagg cgtatttcct ttgtgataat agtcactttt tttaaacaat taagataact
     7321 gtaattaaca attatttact attgcactgt taattggtgc gaggttgtga tgaaagacgt
     7381 tgtgattgtc ggggcgttac ggacacctat cggctgcttt cgtggtgcgt tagcgggtca
     7441 ttccgccgtg gaacttggca gcctggtcgt caaagcgtta atagaacgta ccggcgttcc
     7501 tgcatatgcg gtggatgaag tgattcttgg tcaggtgttg actgcagggg cagggcagaa
     7561 tccggcaagg caatcggcta ttaaaggtgg tcttcctaat agcgtttctg caatcactat
     7621 taatgacgtt tgtggttccg ggcttaaagc actgcatctg gctactcagg cgatacagtg
     7681 tggcgaggct gatattgtca tcgccggtgg ccaggaaaac atgagccgcg caccacatgt
     7741 tctgactgat agccgcaccg gcgcacagct tggcaatagc cagttggttg atagtcttgt
     7801 gcatgatggg ttgtgggatg ccttcaatga ttatcatatt ggtgtcaccg ccgaaaatct
     7861 ggctcgcgaa tatggcatca gccgtcagtt gcaggatgct tacgcactta gctcgcaaca
     7921 aaaagcgcga gcggcgattg acgccggacg atttaaagat gagatcgtcc cggtaataac
     7981 ccaaagtaac ggtcagacgt tggttgttga taccgatgaa cagccacgca ctgacaccag
     8041 cgcagaaggc ttagcccgtt taaatccttc atttgatagt ctcggttctg tgacagcggg
     8101 taatgcatca tccataaacg atggggcagc tgcagtaatg atgatgagcg aagccaaagc
     8161 acgagcgttg aatttacccg tgctggcccg catccgcgca tttgccagcg ttggtgtgga
     8221 tccggcattg atgggaattg cgccggtgta tgcgacccgc cgttgcctgg agcgtgttgg
     8281 ctggcagttg gctgatgtcg atcttatcga ggctaatgaa gcgtttgctg cacaggcgct
     8341 ttcggttggc aagatgcttg aatgggatga gcgtcgggtc aatgttaatg gtggtgcgat
     8401 cgcactcggt cacccgatag gcgcttccgg ttgccgaatc ctggtttctc ttgttcatga
     8461 aatggtgaaa cgtaatgccc gcaaaggact ggcaacgctt tgtatcggcg gaggccaggg
     8521 tgtggcattg accattgaac gtgacgagta gctctgcatt tcctccttac cgataaatat
     8581 ctgccgtcat taatctgttg aataatgatg gctggtgagt cgacatacct tttcagggct
     8641 ccctgatttt tccattctca tgacatattc gtgatcgaca ctgcactttt tttaatcctt
     8701 aacttgaaaa tgaaacattg ttttattttt aattgaaaac cctatcccat gggcatatca
     8761 tcccctcata gagaaaacga aacagtgttt tattattgtt ttctattttc acgcttcact
     8821 gattatcgga ggttgatgtg gacgtaagac agagcatcca cagtgcgcac gcaaaaacgc
     8881 tggataccca ggggctgcgc aatgaatttt tggttgaaaa ggtatttgtc gccgatgagt
     8941 acaccatggt ttacagccac attgaccgta ttattgttgg cggtattatg ccggtaacta
     9001 aaacggtttc cgttggcggg gaagttggta aacaactcgg cgtaagctat ttccttgaac
     9061 gtcgcgagtt aggtgttatc aatattggcg gtgccggtac gattactgtc gatggtcaat
     9121 gctatgaaat cggtcaccgc gacgccctgt atgttggtaa aggtgcaaaa gaagttgtct
     9181 ttgccagtat tgataccgcc actccggcga agttttatta caattgcgca cccgcgcata
     9241 cgacgtatcc caccaaaaaa gtcacaccgg acgaagtatc tccagtcacg ttaggcgata
     9301 acctcaccag taaccgtcgc acgattaaca aatattttgt cccggatgtg ctggaaacct
     9361 gccaattgag tatggggctg acggagctgg ctccaggtaa cttgtggaac accatgccgt
     9421 gtcacaccca cgagcgccgg atggaagttt atttctattt caatatggat gatgacgcct
     9481 gcgttttcca catgatgggg caaccgcaag aaacgcgtca cattgtgatg cataacgagc
     9541 aggcggtgat ctccccgagc tggtcgatcc attccggcgt cggaaccaaa gcttatacct
     9601 ttatctgggg catggtcggt gaaaaccagg tttttgatga tatggaccat gtggccgtta
     9661 aagatttgcg ctagttgtgg gcataaacga ataaggtatt gttatgattt taagtgcatt
     9721 ttctctcgaa ggtaaagttg cggtcgtcac tggttgtgat actgggctgg gccaggggat
     9781 ggcgttgggg ctggcgcaag cgggctgtga cattgttggc attaacatcg ttgaaccgac
     9841 tgaaaccatc aagcaggtca cggcgctggg gcgtcgtttt ttaagcctga ccgccgatct
     9901 gcgaaagatt gatggcattc cagcactgct ggatcgcgcg gtagctgagt ttggtcatat
     9961 tgatatcctg gtgaataacg ccggattgat tcgccgcgaa gacgctctcg agttcagcga
    10021 aacagactgg gacgatgtca tgaacctgaa tatcaagagc gtattcttca tgtctcaggc
    10081 agcggcgaaa cactttatcg cgcaaggcaa tggcggcaag attatcaata tcgcgtcaat
    10141 gctctccttc cagggcggga tccgtgtgcc ttcttatacc gcatcaaaaa gcggtgtgat
    10201 gggcgtgacg cgattgatgg cgaacgaatg ggctaaacac aatattaatg ttaatgcgat
    10261 agctccgggt tacatggcga ccaacaatac tcaacaactg cgggcagatg aacaacgtag
    10321 cgcggaaatt ctcgaccgca ttccagctgg ccgttgggga ctgccgagtg acctgatggg
    10381 gccggtagtg tttcttgcct ccagcgcttc agattatgta aatggttata ccattgctgt
    10441 ggatggcggt tggctggcgc gttaattcat tcct
//
LOCUS       DAZFFE010000137        10409 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFFE010000137 DAZFFE010000000
VERSION     DAZFFE010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.137"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..437)
                     /locus_tag="ACHJ69_004184"
     CDS             complement(<1..437)
                     /locus_tag="ACHJ69_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003833929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017041.1"
                     /translation="MTTYNTGNPLGSSAAKDLYDNAQNFDHLSNDQSNELWPDRFGNP
                     RLTWHGMESKYKESLSKMGWILIKSFQVGAEITLTNQALHDETSGEYYRWDGVLPKTV
                     PAGSTPETTGGIGLGAWVSVGDASLRGDLLSLDGEKYIGICPD"
     gene            complement(490..3207)
                     /locus_tag="ACHJ69_004185"
     CDS             complement(490..3207)
                     /locus_tag="ACHJ69_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity factor TipJ family phage tail
                     protein"
                     /protein_id="HFO5017042.1"
                     /translation="MTIRIYPSRLPGEPLETHEHGAVTIHQWLAKNVQGYKPDMKHPI
                     TVDVDGENIPPQAWFEFAIKSDSDVRIYPVPYGAVALAWIAVAVSVASVAYALFFAPG
                     VGDLGGYSSGTGNPLDVNPAKANNAKLGDPIRELFGRSRIYPDYAVQPVTRFSVDDPT
                     VMTVEMFVVMGRGRFSFGDGDIRVGSTPIASLGDGFNYTVYQPGQSVSGDQRSENWFN
                     STEVGGTASGSGLDMAQTAPDTEDVVANSLTVSGPTITFNGLSTDDGDETTNDLPDTW
                     TVGAIVELIVPDSYVVTNDGAYSRITSDTLEEIAPYVGMPVTLWYNSIDYQLFIADFI
                     PHSEPVGEDVITASITLAYDSATGTPFTGIPEGYVRLSVSHSGSEYKILDIDGSSVTL
                     ERVIDGSVDPSWPGFSPRTVLDFEANGLNENDSWMGPFLACPENEVVDMFEVNFFFPN
                     GICGYNKKGNKQYREVKWEIQYRPYGSGVGWISKTGSYYLQNINGLGFTERITLDTPA
                     LVEVRARRTNEQGQNNSRDNMYWQSLRGRLLSRPASYTGVTTIAVTVETGGKLAAQSD
                     RRVNVVATRVYDSGVSRSISGALYHIGNELGLAMDREAIDSLETTYWTPGSEFFDYAT
                     TDSVSALEMLQKVTNAGKSYFLLTDGLASVAREGVKTWTGIISPQEMTDPLQTAFVAP
                     SADDYDGVDVTYINGTTWAEETVQCRTSDNPTPVKIEDYTLDGVLDQDRAYQIGMRRL
                     MKYLQQRLTHTTTTEMDALCYNVGDRVVFTDDIPGSKTISTLIEDMSTSDGVTTITVS
                     EPLDWTFNNPRALIRYQDGSASDLLAATRVNEYVLSVIEQAKFSNIMLNAPSIESPRL
                     IFCESSRVGYSALISEIAPQSDGTCQVTAKEYRDTFYQYDNATYPGNVA"
     gene            complement(3204..3584)
                     /locus_tag="ACHJ69_004186"
     CDS             complement(3204..3584)
                     /locus_tag="ACHJ69_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016160832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter"
                     /protein_id="HFO5017043.1"
                     /translation="MFNPDKYLSVILQKGGRTYPELDCFGIVNEVRKDLGLPLWPDFS
                     GITKDGGGLNREARKLMLSLEKCAPCIGAGAACYSGSTVTHVGVVVEINGQLHVAECN
                     PGVNVTFLPVSRFKRRFVKVEFWK"
     gene            complement(3594..4076)
                     /locus_tag="ACHJ69_004187"
     CDS             complement(3594..4076)
                     /locus_tag="ACHJ69_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1833 family protein"
                     /protein_id="HFO5017044.1"
                     /translation="MTILNRLYASSGEEVIIETLQINIGTGVYYLCKGFDDITATTES
                     GVVVTFQAAAIDIALPARNSDGTQDLQFAIDNIDGITSTAIRNALDNLSEASLTYRNY
                     VSTDLSAPASVPYTLAIKSGSWTSMQAQITAGYMNVLDTAWPRHRYTLPYYPGLRYMS
                     "
     gene            complement(4073..4546)
                     /locus_tag="ACHJ69_004188"
     CDS             complement(4073..4546)
                     /locus_tag="ACHJ69_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003833936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017045.1"
                     /translation="MAKTTSINYPNDYLPVPLQEGFGLKPVSPLLRTELTSGRARQRR
                     LYTSTPTQASVAWLLTDPEAQLFEVWFRDTIKDGADWFNMPLRSPLGVVDVYVCRFVD
                     IYEGPTIEGGNYWRYTATLELWERPVLAPGWADFPDYIINSSIIDIALNREWPEP"
     gene            complement(4546..7071)
                     /locus_tag="ACHJ69_004189"
     CDS             complement(4546..7071)
                     /locus_tag="ACHJ69_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003833938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5017046.1"
                     /translation="MAGKSLGTLTIDLIAKVGGFVSGLSQAERASQKWRKQVQADAKA
                     AAVAFTGFATAASAAAIGVGVAGYNLLKTTSKQITETDRWAKSLNMSTQSLLAWQYAA
                     EKAGVSGDQMADIFKDIGDKIGDAVLNKSGEAVDALNALGLSAKKLSSESPDKQLLAI
                     SNALGKIKTNAEKTTILESLGNDLSKLLPLLDQGGEKLRQYMDAAKQFGVAPDDADIE
                     KLVKVNSLFEDMETQVNGVKIEIATGLANVDLSGLQNAITDMGDVFKDPQVIQGLTDL
                     VGGVVDLATWLVKVGAEAGKLIDLYKGGKAVGDNASVTDIERRLNNLKTDVEDQGFLA
                     SFNRIGMDVDGKKAEIAQLERRLSIMKAGNNLPLSQATVGAPSSSSKNYSLGSGETNG
                     KASPDAGARKLESAFKSLEMSYQRQIALIDTTGKKNQQVTELEKLRFDFTSGKLTGIN
                     AAQKERLEQLATEIDRLNSLKKANEENLKLVEFTANLRKQNQNDQAANDSDFIGAGMG
                     DKTRQRMKELLDIHRSFLDRQQDLQKQYQSGDISKSLYDQETAALQQALDDRLDIQED
                     YYKKSDAQMGDWQSGIMDSLNDYADKSADYYQTAADAMTSILGGATSVISDNLNDLVH
                     GAEDLGDFFSNIFSGLGETIIKTLSDMAAQWLVYQAVQLLVGKSTQASAAASMLANAQ
                     ASSLQAQIAAYASTAAIPIVGPALAPAAMATAAAVTAPLVAAVGTSALAGMAHDGIDS
                     VPETGTWLLQKGERVVTSQTSAKLDETLDRVNQQSTSGASFSPVINMNVNGDPSDTQI
                     SMMKQAMAEGAKLGYQQAASDLASGKGDISKAMMRWNTNRRTG"
     gene            complement(7339..7716)
                     /locus_tag="ACHJ69_004190"
     CDS             complement(7339..7716)
                     /locus_tag="ACHJ69_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone family protein,
                     TAC"
                     /protein_id="HFO5017047.1"
                     /translation="MKLTLDSLKQAGAFTGRPVEKEITWKQSDKEITATVYIRPMGYH
                     DAVSTVLSAAGKIDGVAGRIASSICDENGNPVFTVADITGEADPERGALDGNLTVALL
                     VAIQQVNDLGKTNSAQKTNSGVN"
     gene            complement(7769..8266)
                     /locus_tag="ACHJ69_004191"
     CDS             complement(7769..8266)
                     /locus_tag="ACHJ69_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007898337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO5017048.1"
                     /translation="MSVVTQGTQMYVLNNGVVSEVECITSFSPGSSPADQIEDTCLSE
                     TNTRSYKKGLRTPGQATVALNADPANASHVMLSNLAESSDQTNLTFAIGWADGTADPT
                     AATVGDPDAVDGLSLPDTRTWYVFQGYVSDFPFDFQANTVVQTSATIQRSGQGVWVPK
                     AQPTS"
     gene            complement(8319..8684)
                     /locus_tag="ACHJ69_004192"
     CDS             complement(8319..8684)
                     /locus_tag="ACHJ69_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO5017049.1"
                     /translation="MIAPIFPVCASSPEVTTLLGSNPVRIYPFGIQDDNVVYPYAVWQ
                     NVSGSPENFLNQRPDADIYSLQVDIYADTPDEAIAVAQAMRNAIEVKANIVRWGNQTR
                     DPETLRYRYSFDVDWIVNR"
     gene            complement(8681..9166)
                     /locus_tag="ACHJ69_004193"
     CDS             complement(8681..9166)
                     /locus_tag="ACHJ69_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003833948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO5017050.1"
                     /translation="MVDSIDFKLEGVDSLLGKLEAITSETKRKTGRSALRKAGNVIVT
                     QIKRNAERLDDPHTARSIADNAALRWNGRMFKQTGDLAFRIGILQGAVLKKHPSTAKD
                     APTPHWRLLEFGTEKMAAKPLVRAAANSRLIEVFNTFSVNYEAGIDRAIKRAQKKGET
                     A"
     gene            complement(9159..9491)
                     /locus_tag="ACHJ69_004194"
     CDS             complement(9159..9491)
                     /locus_tag="ACHJ69_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO5017051.1"
                     /translation="MEFAKLRHRITIQRRTATQSPTTGAMEYTWNDLAEVWGNVVASS
                     VRDFITAQASNVKVTARITIRYREDIQEKDRILFRGKIYSIEGILPDPDSGLEYLTLP
                     CSEGVRDG"
     gene            complement(9494..9688)
                     /locus_tag="ACHJ69_004195"
     CDS             complement(9494..9688)
                     /locus_tag="ACHJ69_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017052.1"
                     /translation="MNEQQVNELTAALKALATSQLKQAEAINRLAQADETLISLIAKT
                     LVDEIDDELPPQTYLDGKPR"
     gene            complement(9759..10085)
                     /locus_tag="ACHJ69_004196"
     CDS             complement(9759..10085)
                     /locus_tag="ACHJ69_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007898361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO5017053.1"
                     /translation="MIELVTLEEAKLHLRIDDDYGDSDLTLKIQGGSAAILSYIQGSR
                     ALVVDDSGKLIDGEPLTRVQTALLVLLGYLDRNRGGEEEEKLKQGELPFSVSMLIYDL
                     RKPTII"
     gene            complement(10082..>10409)
                     /locus_tag="ACHJ69_004197"
     CDS             complement(10082..>10409)
                     /locus_tag="ACHJ69_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007898363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFO5017054.1"
                     /translation="HEKTHGMRGWECIVDGVSGFDIKQDNQRTFSISLERASGTVEVK
                     SFDIPVTIYRDVFKSGTEYQPGDTVTWGGCMWHCNEKTCDKPGETGSKGWTLAVKKGR
                     DLRDKP"
BASE COUNT         2616 a         2641 c         2319 g         2833 t
ORIGIN      
        1 atgtcaggac agataccgat atatttttca ccatctaagc tgagtagatc tccacgcaat
       61 gaagcatcac ccacgctaac ccacgcacct aatccaatcc cacctgtcgt ttctggcgta
      121 gagccagcag gaacggtttt aggtaataca ccatcccaac ggtaatactc gccagaggtt
      181 tcgtcgtgca atgcctgatt agtcaaggtt atctctgcgc caacctgaaa tgattttatt
      241 agtatccaac ccatttttga caatgattct ttgtactttg actccatacc atgccaagta
      301 agacgtggat taccaaaacg atcaggccat aactcattgg attgatcatt agaaagatga
      361 tcgaagttct gggcgttatc gtacaaatct tttgcagcag aagaccccag cggattgcca
      421 gtattataag tcgtcatatt tgcctcataa gagaaaaacc cgccgtagcg ggtaatattg
      481 atgtttggat taagctacat tgccggggta ggtagcgtta tcgtactgat agaaagtgtc
      541 gcgatactct tttgccgtta cctgacatgt tccatcagat tgcggtgcta tctctgaaat
      601 taatgcgcta tatccaacac gggatgactc gcagaaaatc agacgaggcg attcaatgga
      661 tggggcgttt agcatgatat tgctgaattt tgcttgttct attactgata gaacatattc
      721 atttaccctt gtagctgcca gcaaatccga agcagaacca tcctgatagc gtatcaatgc
      781 acgtgggttg ttgaatgtcc agtcaagtgg ttctgatact gtaatagtcg ttacgccatc
      841 acttgtactc atatcctcaa tcaacgtgct tattgttttg ctaccaggaa tatcatccgt
      901 gaatactacc ctgtcgccaa cgttataaca gagagcatcc atttccgttg ttgtggtatg
      961 agtcaaacgt tgctgaaggt acttcatcag acgtcgcata cctatctgat aagcacggtc
     1021 ctggtcaaga acaccatcaa gcgtatagtc ctcaatcttt accggagttg ggttatctga
     1081 tgttctgcac tgcaccgtct cttcagccca tgtagtgccg ttgatatatg taacatccac
     1141 accatcatag tcatctgctg acggagcaac gaaagcagtc tgaagcggat cagtcatctc
     1201 ctgtgggctg attattccag tccacgtttt cacaccttcc cttgctacag atgccagacc
     1261 atcggtaaga aggaagtaac tttttcctgc atttgtcacc ttctgaagca tctccagcgc
     1321 agaaacagaa tccgtagtgg catagtcaaa gaattcactg ccaggggtcc agtaagtagt
     1381 ttctagtgag tcaatagctt cacggtccat cgctagcccc aactcattac ctatatgata
     1441 cagagcacca gaaatgctcc ttgacacacc agaatcataa actcgcgtgg ctacaacgtt
     1501 tacgcggcga tctgactgcg ctgccagttt accgccagtt tccacagtaa ccgcaattgt
     1561 ggtgacgcca gtatacgatg ctggccgtga caacaaacgt cctcgcaatg actgccagta
     1621 catgttatcg cgactgttat tctgtccctg ctcgttcgtg cggcgagctc tcacctctac
     1681 tagcgcaggc gtatccagcg tgattctttc ggtaaatccc agtccgttaa tgttctggag
     1741 gtagtacgat ccggttttac ttatccaccc gacgcctgaa ccatatggcc ggtactgaat
     1801 ctcccacttt acctcacggt attgtttgtt cccttttttg ttatacccac aaatcccgtt
     1861 tgggaagaag aagtttactt cgaacatatc aacaacttca ttttctgggc acgccagaaa
     1921 aggtcccatc caactatcgt tctcatttaa accgttggct tcaaaatcaa gaactgtgcg
     1981 cggtgaaaat ccaggccatg acggatcaac gctgccatca ataacccgct ccagagttac
     2041 agagctacca tctatatcaa gaatcttata ttcgcttccg gaatgggaaa cagatagcct
     2101 gacataacct tcaggaatcc cggtgaacgg agttccagtg gcgctatcgt aagcgagggt
     2161 tattgatgcg gtaatcacat cttcgccgac tggctcagag tgaggtataa aatcagcaat
     2221 gaatagttgg taatcaatgc tgttgtacca gagagttaca ggcatcccaa cataaggagc
     2281 tatttcctcc agcgtgtcac tggtaattct gctgtaagca ccatcattag ttaccacata
     2341 ggaatcagga acaattaact caacaattgc accaacagtc catgtatctg gaagatcatt
     2401 tgttgtttca tctccatcat ccgtgctcag cccattaaat gttattgttg gtccggaaac
     2461 ggttagcgaa ttagcaacaa catcttccgt gtccggcgcg gtttgcgcca tatcaagacc
     2521 agaaccagat gcggttcccc caacctctgt ggaattgaac cagttttctg atcgttgatc
     2581 tccactaacg ctttgcccgg gttgatacac tgtgtagtta aacccatcac caagagaagc
     2641 aattggagtt gaccctacac gaatatcgcc atccccaaat gaaaaacgcc ctctccccat
     2701 aacaacaaac atttcaactg tcattaccgt tggatcatca actgagaatc ttgttacagg
     2761 ctgcactgca tagtcaggat aaatacggct tcgtccaaat aactcacgta tcgggtcgcc
     2821 caattttgcg ttattcgctt tagcaggatt tacatcaaga gggtttcccg ttcctgatga
     2881 ataaccgcca aggtcaccaa caccaggagc aaagaaaaga gcatacgcga ctgatgcaac
     2941 tgatacagca acggcaatcc aggcaagagc tacagcgcca taagggacag ggtaaattct
     3001 gacatcactg tctgatttga tagcaaattc aaaccatgcc tgaggcggta tgttctctcc
     3061 atcaacatca acggtgatcg ggtgcttcat atcaggctta tagccctgaa cgttcttcgc
     3121 aagccactga tggatagtta ctgcgccatg ttcatgagtt tcgagtggct cgccaggcag
     3181 ccgtgaagga taaattctga tagtcacttc cagaactcca ctttgacaaa acgacgtttg
     3241 aaacgcgaga caggaaggaa ggtaacgttc actcctgggt tgcactctgc gacatgcaat
     3301 tggccgttaa tttcaacgac tacaccaaca tgagtaacgg ttgacccgga ataacaggcg
     3361 gcaccggcac ctatacacgg cgcgcatttt tccagagaaa gcatgagctt tctggcctct
     3421 cggtttaaac ctccgccgtc ttttgttata cctgaaaaat ctggccacag tggtagcccc
     3481 aggtcttttc gtacctcgtt cactatgccg aagcagtcaa gttcaggata agtgcgaccg
     3541 cccttctgca atatgacaga aaggtattta tcaggattga acattgttgc tccttaactc
     3601 atgtaacgaa ggccggggta atacgggagg gtgtatcggt ggcgcggcca tgcggtatca
     3661 agaacgttca tatatccggc agtaatctgc gcctgcatgg atgtccatga cccgctttta
     3721 atcgccagcg tgtacggtac agatgccgga gcactgaggt ccgtggagac atagttacga
     3781 tatgtcagcg atgcctctga aaggttatcc agagcattcc gtatggcggt ggaagtaatc
     3841 ccgtctatgt tatcaatggc aaactgcaaa tcttgtgtac catcgctgtt tcttgctgga
     3901 agggcaatat caatagccgc agcctgaaag gttaccacca cgccactttc ggttgttgcg
     3961 gtgatgtcat cgaagccttt acaaagataa taaacaccag taccgatgtt tatttgcagg
     4021 gtttcgatga tcacctcttc cccggatgag gcatacagcc tatttagaat cgtcatggct
     4081 caggccactc cctgttaagc gcaatatcaa tgatgctgct gttgataatg taatcaggaa
     4141 aatcagccca gccaggagcc agaacaggcc tttcccacaa ttccaacgtg gccgtgtagc
     4201 gccagtagtt tccgccttca attgtcggcc cctcatagat atccacgaaa cgacagacat
     4261 aaacatcaac aacgcctaac ggagatcgaa gaggcatgtt aaaccagtca gcgccatcct
     4321 taatggtgtc cctgaaccat acctcaaata gttgagcttc cggatccgta agcagccatg
     4381 caaccgaggc ctgagttggc gttgaagtat ataatctcct ctgcctcgcc ctgccggatg
     4441 tgagttcggt tctcagtaat gggctaactg gctttaaccc gaacccttcc tgcaatggaa
     4501 caggaaggta atcattcgga tagttaatgc tggtcgtttt agccattaac cagttctcct
     4561 gttagtgttc cagcgcatca ttgctttcga aatgtcacct ttcccactgg caaggtcgct
     4621 ggctgcctgt tgatatccaa gttttgcccc ctctgccatg gcttgcttca tcatggagat
     4681 ctgagtatca gacggatcac cgttaacatt catgttaata acgggagaga agcttgctcc
     4741 gctggttgat tgttgattta ctctgtccag tgtttcatca agctttgctg acgtctgaga
     4801 ggtcacaact cgctcacctt tctggagcaa ccatgttcct gtttccggga cgctatcaat
     4861 accatcgtgc gccatacctg ccagggcaga agtaccaact gctgcaacaa gtggtgcagt
     4921 tactgccgca gctgttgcca tcgctgcagg tgcaagcgcg ggaccaacga taggaattgc
     4981 cgctgtcgat gcgtatgctg cgatttgagc ctgcaaagat gatgcctgtg cgtttgccag
     5041 cattgatgcc gctgcgcttg cctgagtgga tttacctacc agcaattgca ctgcctgata
     5101 taccagccat tgcgccgcca tatcagaaag ggttttaatg attgtttcac caagaccaga
     5161 gaaaatatta ctgaagaaat cacctaaatc ttctgcaccg tgcacaaggt cattaaggtt
     5221 gtcagaaata accgaggttg ctccaccaag aatagaagtc atcgcatctg cagcagtctg
     5281 gtaataatca gctgatttat cagcataatc gttcaacgag tccattatcc cgctttgcca
     5341 gtcgcccatc tgagcatcag actttttgta gtagtcctcc tgaatatcaa gacggtcatc
     5401 aagcgcctgc tgtaacgctg ccgtttcctg gtcataaagc gatttgctta tatcaccact
     5461 ttggtactgt ttctgtagat cttgctgcct gtcgagaaaa ctacgatgga tatccagcaa
     5521 ttccttcatg cgctggcgag tcttgtctcc catacctgcg ccgataaaat cagaatcatt
     5581 agctgcctgg tcattctgat tttgcttacg taaattagcg gtaaattcga caagtttcag
     5641 attttcttcg ttggcttttt taagtgagtt gaggcgatct atctcagtag caagttgctc
     5701 aagtcgctct ttctgagctg cattaattcc tgttaatttc ccagatgtaa aatcaaaacg
     5761 cagcttctca agctcggtta cctgctgatt ctttttgcca gttgtgtcaa tcagcgctat
     5821 ctgacgctga tagctcattt ccagagactt aaatgctgac tccagttttc tggccccggc
     5881 atctggagat gctttcccgt tagtttctcc tgagcctaag gaataatttt tgctggaaga
     5941 agaaggcgca cctacagtgg cctgactgag aggaagatta ttaccggctt tcatgatgga
     6001 cagtcggcgt tctaattgcg ctatttcagc ttttttcccg tcaacgtcca taccaattcg
     6061 gttaaagctg gcaaggaatc cctgatcttc tacatcagtt ttaagattgt tgaggcggcg
     6121 ctctatatca gttacagatg cattgtcacc tacagcctta ccgcctttgt acaggtcaat
     6181 tagcttccct gcttcagcac caactttaac aagccaggtc gcgagatcaa caacaccacc
     6241 aaccaggtct gtcaaaccct gaataacctg aggatctttg aatacatccc ccatgtcagt
     6301 tatcgcgttc tgtaaccctg acaagtcaac attggccagg ccggtggcaa tctcaatttt
     6361 tacaccgttt acctgcgtct ccatgtcctc aaacagggag tttactttta ccagcttttc
     6421 gatatcagca tcgtcagggg caacgccaaa ctgttttgcc gcgtccatgt actgacgaag
     6481 tttttctcct ccctgatcaa gcaatgggag aagcttggaa aggtcgttac caagactttc
     6541 aaggattgtg gtcttttcag cgtttgtttt aatcttgccg agcgcattgc tgatagccag
     6601 caactgctta tctggtgatt cactggataa tttcttagcc gataatccga gtgcattcag
     6661 agcatctact gcttcacccg atttattcag tacagcatcg ccaattttat cgccaatatc
     6721 cttgaagata tcggccatct ggtcgccgga tacgccagct ttttcagctg catactgcca
     6781 ggcaagcaat gactgcgtag acatattgag cgacttagcc caacggtctg tttcggtaat
     6841 ctgttttgac gttgttttta gcaggttgta accggcgaca ccgacaccga tagctgccgc
     6901 acttgccgcg gtagcaaaac cagtaaatgc taccgcagcc gcttttgcat cagcctgaac
     6961 ctgttttcgc catttttgag atgctcgttc agcctggctt agaccagata caaatccacc
     7021 aaccttagct atcaggtcaa tggtcagcgt tccgagggat ttaccagcca tgctatgtcc
     7081 actcctgcat agcctgctcc agtgaaatgg aaggctcgtt aatgtgtggt gtgaagtctg
     7141 ttactttgaa ggagggggaa tcttttccac ggttgacgtt agccagcaca gaagaaataa
     7201 ggcctgcggc ccactcagta cgcatcattc cgttaaggct accgtacttt tgacggtaaa
     7261 gaacccagtc gcgatactct gtaacgctaa cgcgttcttt tgcttcagca atggtgcgac
     7321 cgccgatccc gttgagaact aattcacacc agaattcgtc ttctgcgctg agttcgtctt
     7381 tcccaggtcg ttaacctgct gaatagcgac aagtagagcg acggtaaggt ttccgtcgag
     7441 cgcaccgcgc tcagggtctg cttcaccagt aatgtcagca acagtaaaaa caggattccc
     7501 gttctcatca caaatggatg atgcgatacg tccggcaaca ccatcaatct ttcccgcggc
     7561 tgacagaaca gtagatactg cgtcgtgata acccataggt cggatataaa cggtagcggt
     7621 aatttcctta tcactctgct tccaggtaat ctccttctca acaggtctcc cagtgaatgc
     7681 accagcctgt tttaatgaat ccagagtcag tttcatttat tactctctta ttgcggggtt
     7741 acccccgcta gttggcagat tattacgttt agctggttgg ctgtgcttta ggaacccaga
     7801 cgccctggcc ggaacgctga atagtcgcgg aagtctgaac taccgtattg gcctgaaaat
     7861 caaacgggaa atctgataca taaccctgga atacatacca ggtacgggta tctggaagag
     7921 acagaccatc aactgcatca gggtcaccga ctgttgcagc agtaggatct gcagtaccat
     7981 cggcccagcc tatagcgaag gtcaggttgg tctggtcgct tgattcagcc aggttgctca
     8041 gcattacgtg actggcgttt gccgggtcgg cgttaagagc aacggttgcc tgacccgggg
     8101 tgcgcagacc ttttttataa gaacgggtat tggtttcgct cagacaggta tcttcaattt
     8161 gatctgccgg gctactacct ggtgagaatg aagtaataca ttcaacttca ctgaccacgc
     8221 cgttattcag aacgtacatc tgagtgcctt gagtcactac tgacataatt atctccgggt
     8281 ataaaaaaac cggctcaagg ccggttgtgg aaggtttgtt atcggttgac tatccagtca
     8341 acgtcgaaag aatatcgata cctgagcgtc tcagggtctc gcgtctgatt accccagcga
     8401 acaatgttgg cttttacctc aatcgcatta cgcatggctt gagctacagc aatcgcctca
     8461 tcaggggtat cggcatagat atcaacctga agcgaataaa tgtccgcatc tggtcgttgg
     8521 ttaaggaaat tttcaggaga gccgctgacg ttctgccaga cggcgtatgg ataaacaaca
     8581 ttatcgtcct gaattccgaa agggtaaatt cttaccggat tgcttccgag taaagtagtg
     8641 acttcaggac tcgacgcgca aacaggaaaa ataggagcaa tcatgccgtc tctcctttct
     8701 tctgtgctcg tttgatagct cggtcaatcc ccgcttcata gttaacagag aaggtgttga
     8761 aaacctctat cagtctggaa tttgcagcag cacgaacgag cggttttgct gccatctttt
     8821 cagtgccaaa ctcaagaaga cgccagtgag gagtgggggc atctttcgca gtgcttggat
     8881 gcttttttaa aacggctccc tgaagaatgc ctatcctgaa ggcaagatca ccggtttgtt
     8941 taaacatacg accattccag cgcagcgcgg cgttatcagc aatgctacgt gcggtgtgag
     9001 gatcgtcgag tcgctctgcg tttctcttta tctgagttac gataacgttt cctgctttcc
     9061 tcagtgcgga gcgccctgtt ttgcgcttag tttctgaggt aatggcttct aacttaccaa
     9121 gcagtgaatc tacaccttca agcttaaaat caatgctatc aaccatccct taccccctct
     9181 gagcacggaa gcgtaagata ttcgagccca ctatcagggt caggaagaat cccctcaatg
     9241 ctgtagattt tgccacggaa aagaatacgg tctttctcct ggatatcttc ccggtatcgg
     9301 atagtgattc tggccgttac ttttacgttt gatgcctggg ctgtaatgaa atccctgacc
     9361 gatgaggcaa ccacgttacc ccacacctct gcaaggtcat tccaggtgta ttccattgcc
     9421 cctgtggttg gtgactgggt ggctgttcgt cgctgaatag tgatgcggtg acgcagttta
     9481 gcaaactcca cgattacctc ggcttaccat caagataggt ctgcggtggt agctcatcat
     9541 caatttcatc aacgagcgtt ttggcaatca gagatatcag tgtttcatcc gcctgagcca
     9601 gacggtttat cgcttctgcc tgtttcagtt gagacgttgc cagcgctttc agtgccgccg
     9661 ttagttcgtt tacctgctgc tcgttcatag gcaatcctcg tccacttttt aatccactcg
     9721 cgccgtctgg cacatcctgc gcatgccata catgcccctt aaataattgt cggcttacgg
     9781 aggtcgtaaa tcagcattga tacagaaaaa ggaagttccc cctgtttcaa cttttcttct
     9841 tcttcgccgc cgcggttacg gtcaagatag ccaagaagaa ccagcagggc cgtctgaacg
     9901 cgagttagcg gttcaccatc aataagcttt cctgaatcat ccacgactaa cgcgcgactt
     9961 ccctgaatgt aggaaagtat tgctgcgctg ccgccctgaa tttttaaggt aaggtctgaa
    10021 tctccgtaat cgtcgtcaat acggagatgc aacttagcct cttcgagcgt caccagttca
    10081 atcatggctt atccctcaga tctcgccctt tcttaacagc aagcgtccat cctttcgatc
    10141 ctgtctcacc tggcttgtcg catgttttct cgttgcaatg ccacatacaa ccaccccatg
    10201 tgaccgtatc gccaggttga tattcggtac cagatttgaa tacatcgcga tagatagtta
    10261 ccgggatgtc aaaggactta acttcaacag taccgcttgc tctttcgaga gaaattgaga
    10321 aggttcgctg gttatcttgt ttgatatcaa agcccgatac accgtcaaca atacattccc
    10381 agccccgcat accgtgcgtc ttttcatgg
//
LOCUS       DAZFFE010000138        10305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFFE010000138 DAZFFE010000000
VERSION     DAZFFE010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.138"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..713
                     /gene="gspC"
                     /locus_tag="ACHJ69_004198"
     CDS             <1..713
                     /gene="gspC"
                     /locus_tag="ACHJ69_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO5017055.1"
                     /translation="NYFHTTIVKNEQIINQPTNAFQSEFSLAALWRNENHAGVKDANP
                     VAVNQETPKLSIALNGIVLTSNDETSFVLINEGNEQKRYSLNEALESAPGTFIRKINK
                     TSVVFETHGHYEKVTLHPGLPDIIKQPDSENQNVLADYIIATPIRDGEQIYGLRLNPR
                     KGLNAFTTSLLQPGDIALRINNLSLTHPDEVSQALSLLLTQQSAQFTIRRNGVPRLIN
                     VSVAELTGMNGQRNEGTQ"
     gene            697..2649
                     /gene="gspD"
                     /locus_tag="ACHJ69_004199"
     CDS             697..2649
                     /gene="gspD"
                     /locus_tag="ACHJ69_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO5017056.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNSRKPSSTSAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPVFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQLRIDGKSK
                     ALVGSEDLPVLNENTFNSHAPAPSSR"
     gene            2659..4140
                     /gene="gspE"
                     /locus_tag="ACHJ69_004200"
     CDS             2659..4140
                     /gene="gspE"
                     /locus_tag="ACHJ69_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO5017057.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSQAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQKVIRGDTSWDEVMRVASATLENEA"
     gene            4137..5333
                     /gene="gspF"
                     /locus_tag="ACHJ69_004201"
     CDS             4137..5333
                     /gene="gspF"
                     /locus_tag="ACHJ69_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO5017058.1"
                     /translation="MNYRYRAMTLDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNNRLADVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTTVAIVVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQHTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLSNLEIRKRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRISLTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5343..5780
                     /gene="gspG"
                     /locus_tag="ACHJ69_004202"
     CDS             5343..5780
                     /gene="gspG"
                     /locus_tag="ACHJ69_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO5017059.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHRYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5788..6297
                     /gene="gspH"
                     /locus_tag="ACHJ69_004203"
     CDS             5788..6297
                     /gene="gspH"
                     /locus_tag="ACHJ69_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO5017060.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDAASTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESKND
                     WGEELSIQLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVSSGSWPLD
                     QTLARDTRP"
     gene            6294..6671
                     /gene="gspI"
                     /locus_tag="ACHJ69_004204"
     CDS             6294..6671
                     /gene="gspI"
                     /locus_tag="ACHJ69_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO5017061.1"
                     /translation="MNKQSGMTLLEVLLAMSIFTAVALTLMSSMQGQRTAIERMRNET
                     LALWIADNQLQSQDSFDEENTSSSGKELINGEEWNWRSDIHSSKDGTLLEHTITVTLP
                     SGQTTSLTRYQSINNKSGQAQDD"
     gene            6664..7251
                     /gene="gspJ"
                     /locus_tag="ACHJ69_004205"
     CDS             6664..7251
                     /gene="gspJ"
                     /locus_tag="ACHJ69_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO5017062.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL
                     LTVHWYLRNHTLYRAVRTSVDGRKDQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     KTQQYGALQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7244..8227
                     /gene="gspK"
                     /locus_tag="ACHJ69_004206"
     CDS             7244..8227
                     /gene="gspK"
                     /locus_tag="ACHJ69_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO5017063.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTISFHGQLHRTRQVNHH
                     LQRQYDIELAEKLALASLTLDVKDNDRQTTLQQYWAQPQQLQLENGNTVKWQLRDAQH
                     CFNLNALAKISDAPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDADDSPRF
                     HGAEDNFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTSELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPGEGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKGHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            8242..9405
                     /gene="gspL"
                     /locus_tag="ACHJ69_004207"
     CDS             8242..9405
                     /gene="gspL"
                     /locus_tag="ACHJ69_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO5017064.1"
                     /translation="MPESLMVIRSFSTLRKHWEWMTFSADSVSSVHTLTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDASQNWHWTVVDK
                     QNESVEVIGIKSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLANQFPPENMRCYGVAPHGVAAANPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGVFRKQKTVSKSGKWLARLAVSCLVLAILSFVGSRGIAFWQTLKIEDQL
                     QQQQQETWQRYFPQIKHTHNFRFYFKQQLAQQYPEAVPLLYHLQTLLLEHPELQLMEA
                     NYSQRQKSLTLKMSAKSEANIDRFCELTQSWLPMEKTEKDPVSGVWTVRNSGK"
     gene            9402..9863
                     /gene="gspM"
                     /locus_tag="ACHJ69_004208"
     CDS             9402..9863
                     /gene="gspM"
                     /locus_tag="ACHJ69_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFO5017065.1"
                     /translation="MIKSWWAEKSTSEKQIVAALAVLSLGVFCWLAVIKPIDTYIEEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLTITLENGPDNTL
                     TINPVTAPLENVSRWLTTAQITYGIVIEDLQFTLAGNEEITLKHLSFREQQ"
     gene            9863..>10305
                     /locus_tag="ACHJ69_004209"
     CDS             9863..>10305
                     /locus_tag="ACHJ69_004209"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFO5017066.1"
                     /translation="MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHF
                     WQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFALTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLV"
BASE COUNT         2707 a         2638 c         2484 g         2476 t
ORIGIN      
        1 tgaattattt ccatacaact atagttaaaa atgaacagat cattaatcaa ccaacaaatg
       61 catttcagtc tgaattcagc cttgccgctt tatggcgcaa cgaaaatcat gctggggtaa
      121 aagatgcaaa tcccgtggca gttaatcagg aaacacctaa gctctctata gcgcttaatg
      181 gcattgtact cacatcgaat gatgagactt cttttgtctt aattaatgag gggaacgaac
      241 aaaagcgata ctccctcaac gaagcccttg agtcagcgcc gggaacgttt attagaaaaa
      301 taaataaaac cagcgttgtg tttgaaactc acgggcatta cgaaaaagta actctccatc
      361 cagggctacc tgacatcatc aagcagcctg attcggaaaa tcaaaatgta ctcgccgatt
      421 atattatcgc cacacctata cgtgatggag aacagatata cggtcttcga ttgaatcctc
      481 gtaagggtct caatgcattt accaccagct tgctgcaacc tggggatatc gccctcagga
      541 tcaataatct ttcgctgacc caccctgatg aggtttcaca ggcattaagt ttactgctaa
      601 ctcaacaaag tgctcagttt acaattcgtc gcaacggcgt accccgcttg ataaatgttt
      661 ccgtcgcgga acttacagga atgaatggac agcgcaatga agggactcaa taaaatcacc
      721 tgctgcttgc tggcagcact actcatgcct tgtgcaggac acgctgagaa cgaacaatac
      781 ggcgctaact tcaataacgc cgatatccgc cagttcgtgg aaatagtggg tcagcatctt
      841 ggcaaaacga tcctgatcga cccttcggta cagggaacca tttccgtacg cagtaatgat
      901 acgtttagcc aacaggagta ctaccagttc tttttaagta ttcttgatct ttacggttat
      961 tccgtgatca cactggacaa tggttttctg aaagtggtgc gctcagctaa tgtaaaaaca
     1021 tcgccaggga tgattgctga cagttctcgt ccaggcgtag gtgatgagtt ggtcacccga
     1081 atcgtaccgc ttgagaacgt tcctgctcgt gacctggctc ccctgctccg ccagatgatg
     1141 gatgcgggta gcgtcggtaa tgttgtgcat tatgaaccct ccaacgttct tattctgacc
     1201 ggtcgtgcct ccaccattaa taaactgatt gaagtcataa agcgcgttga tgtcatcggc
     1261 acagagaagc agcaaattat tcatctggaa tatgcatcag cggaagatct cgccgagatt
     1321 cttaatcaat taatcagcga aagccacggt aaaagccaga tgcctgccct cctttccgcg
     1381 aagattgtgg cggataagcg aaccaactct cttatcatca gcgggccgga aaaagcacgc
     1441 cagcgcataa cttcattact gaaaagcctt gatgtagaag agagcgagga aggaaatacc
     1501 cgggtctatt acctgaaata tgctaaagcc acgaatctgg tggaagtgct aaccggtgtt
     1561 tccgaaaagc tgaaagatga aaaagggaat tcgcgtaagc cttcttctac ttctgcgatg
     1621 gataatgtgg ccattaccgc cgatgaacag accaactcgt tggtcattac cgctgaccag
     1681 tccgtccagg agaaactcgc cacggtaatt gcgcgtctgg atattcgccg tgcacaggtg
     1741 ctggttgagg caatcatcgt cgaagttcag gatggaaatg gactcaacct cggcgtgcaa
     1801 tgggcgaata aaaacgttgg cgcacagcaa tttaccaata cgggattacc ggtttttaac
     1861 gctgcgcaag gtgtagctga ttataaaaag aatggcggga tcaccagcgc gaatcctgcc
     1921 tgggatatgt ttagcgccta caatggcatg gctgcaggct tcttcaatgg cgactggggc
     1981 gtattgttga ctgcgctggc cagtaacaat aaaaatgaca tcctcgccac cccgagcatc
     2041 gtaacgctgg ataataaact cgcgtccttc aacgttggtc aggatgtgcc ggtgctatcc
     2101 ggctctcaga ccacttctgg ggataacgtc tttaatactg tcgaacgcaa aacggtgggc
     2161 acaaaactca aagtcactcc acaggtcaat gaaggcgacg cggtactgct cgaaatagag
     2221 caggaggttt ccagcgttga ctcttcgtct aattcgacgc tcggcccgac atttaacacc
     2281 cgtactattc aaaacgccgt gctggttaaa accggtgaaa cggtggtcct gggcggattg
     2341 ctggatgatt tttctaaaga gcaagtgtca aaggttcctc tgcttggcga tattccttta
     2401 gtgggacaac tcttccgcta tacctcgacc gagcgcgcta aacgcaacct gatggtattt
     2461 atccgcccga cgattatccg tgacgatgat gtttatcgct cactgtcaaa agagaaatac
     2521 acccgctacc gtcaggagca acagctgcga atcgacggga aatcaaaagc gctggttggc
     2581 tcggaagatt tgccggtgct gaatgagaac acgttcaaca gtcacgcccc tgcgccatcg
     2641 tcacggtgag gcattcttat gagaattcac tcaccgtacc ccgccagttg ggcgctggca
     2701 caacgaattg gttatctcta ttcagagggc gagattattt atctcgccga tacgccattc
     2761 gaacggttac tcgatattca acgtcaagtt ggccagtgcc agaccatgac cagcttgtca
     2821 caggctgatt ttgaagcccg actggaagcg gtgttccatc agaataccgg tgagtcgcaa
     2881 cagattgcgc aggatatcga tcaatccgtc gatcttctct cgctttcgga agagatgccc
     2941 gcaaatgaag atctcctgaa tgaagattca gcggcaccgg ttatccgctt gatcaatgct
     3001 attttgagtg aggccatcaa agaaaccgcc tccgatatcc acattgaaac ctatgaaaaa
     3061 acaatgtcga tccgttttcg catcgacggc gttttgcgga caattttaca gcccaacaaa
     3121 aaactggcgg cactgcttat ctcccgaatt aaggtcatgg cgcgtcttga tatcgccgaa
     3181 aaacgtattc cacaggatgg aagaatcagt ttgcgtatcg ggcgacgtaa catagatgtc
     3241 cgcgtatcca cactgccatc catctatggt gaacgtgccg tgctccgcct gctggataaa
     3301 aacagcctcc agctttcatt gaacaacctg gggatgacgg cagcggataa acaggattta
     3361 gaaaatctca ttcagcttcc gcacggtatt atcctggtaa cagggccgac aggctccggt
     3421 aaaagcacca cgctctacgc cattctttcg gcgcttaata cccctggccg taatattctg
     3481 acggtagaag atcccgtgga atatgaactg gaaggcatag gacaaactca ggtgaatacg
     3541 cgcgtggata tgtctttcgc tcgcggcctg cgcgccatac ttcgccagga cccggatgtc
     3601 gtcatggtgg gggagattcg tgatacagaa accgcgcaaa ttgcggttca ggcctcgctc
     3661 acaggccatc tggtactctc aacactccac actaacagtg catcaggcgc agtcacccgg
     3721 ctacgcgaca tgggcgttga atcattcctg ctttcgtctt cacaggcagg gattatcgcg
     3781 caacgtctgg ttcgtcgtct gtgtccgcaa tgccgacaat tcacgcccgt atcgccccaa
     3841 caagcgcaga tgtttaaata tcatcagctc gcggtgacaa caattggcac tcccgtaggt
     3901 tgccctcatt gccatcaatc cggttatcag gggcgcatgg cgatccacga aatgatggtg
     3961 gtgacgccgg aattgcgggc cgctattcat gaaaatgtgg atgaacaagc actggagcga
     4021 ctggtccggc aacaacacaa tgccttaatc aaaaatggcc tgcaaaaagt gatacgcggt
     4081 gacacctcct gggatgaggt tatgcgcgtc gccagtgcca cgctggagaa cgaagcatga
     4141 attatcgcta tcgcgccatg accctggatg gtcaaaaatt gcaggggatc attgatgcta
     4201 acgatgaacg tcaggcacga ctgcggctgc gtgaagaagg gcttttcctg ttggatattc
     4261 gcccccaaaa aagttcggga gtaaaaacac gtcgcccgag gatcagccat agtgaactga
     4321 cgcttttcac ccggcagttg gcaaccttaa gcgcagcggc attacccctg gaagagagcc
     4381 ttgccgtaat tggtcaacaa agcagtaata atcgactggc tgacgtgtta aatcaggtac
     4441 gcagcgccat ccttgaaggg catccccttt ccgatgcgtt acaacatttt cccacgcttt
     4501 tcgattcgct ctaccgtacc ctggtaaaag cgggcgaaaa gagcggtctg ctggccccgg
     4561 tgttggaaaa gctggctgat tacaatgaaa accggcagaa aatccgcagc aagctcattc
     4621 agtcactgat ctacccctgc atgctcacta cggtggcgat tgtggtcgtg attattctcc
     4681 tcactgctgt cgtgcccaaa attaccgaac agtttgtgca tatgaagcag caactgccac
     4741 tgagtacacg cattctttta ggtctgagcg acacgttgca acataccggc ccgacattac
     4801 tagcgacagt gtttattgtc gctgtaggtt tctggctctg gttaaaacgc ggcaataacc
     4861 gccaccgttt tcatgccatg ttgctgcgcg ttgcactcat cggcccgctg atttgcgcca
     4921 ttaacagcgc acgctatctc cgcactttaa gtattttgca atccagcggc gtccctctgc
     4981 tggatgggat gaatttgtcc accgaaagtc tcagcaacct cgaaatccgc aagcgtctgg
     5041 caaatgcggc agaaaacgtt cggcagggta acagcattca tctttcgctg gaacaaaccg
     5101 caatcttccc gccgatgatg ctctacatgg tggcctctgg cgaaaaaagc gggcagctcg
     5161 gcacattgat ggtcagggcc gcagataacc aggagacact ccaacaaaat cggatctcct
     5221 taacgctctc catcttcgag cccgcactca ttattacgat ggcactgatc gtcctgttta
     5281 ttgtcgtctc ggtactccaa cctcttcttc aacttaactc aatgattaac taaggaaaaa
     5341 caatgcgcgc aacggataag caacgcggtt ttacattact ggaaattatg gtggtgatcg
     5401 tcattatcgg cgtacttgcc agcctggtgg ttcccaacct gatgggcaat aaagaaaagg
     5461 cggataaaca aaaagccgtc agcgatattg tggcgctgga aaacgccctg gatatgtaca
     5521 agctcgacaa tcatcgctat ccaaccacaa atcaggggct tgaatcctta gtcgaagcgc
     5581 cgacactgcc accgctggct gcaaactata acaaagaagg ttatatcaag cgtctgcccg
     5641 ccgatccctg gggcaatgat tatgtccttg ttaatcctgg tgaacacggc gcatacgatc
     5701 tgctttcagc agggccggac ggtgaaatgg gaaccgagga cgacatcacc aactggggtt
     5761 tgagcaagaa gaaaaaataa gtcagcgatg aatcagcaac gcgggtttac cctgctggag
     5821 atgatgctgg tgctggcgct ggtcgctatc acggcaagtg tggtgctctt cacatatggt
     5881 cgggaggatg ccgccagcac tcgtgcgcgc gaaacagcgg cgcggtttac cgctgcactt
     5941 gaattagcca tcgaccgcgc cacgctcagt ggtcagcctg tcggtatcca tttctctgat
     6001 tcagcatggc gcattatggt accgggcaaa acaccatcag cctggcgctg ggttccatta
     6061 caagaagatg ccgcagacga gagcaaaaat gactggggtg aagaactctc aattcaactt
     6121 cagccattta aacctgatga cagcaaccaa ccgcaggtgg tgattctggc cgacggacaa
     6181 atcactccct tctctttgtt gatggctaat gccgggaccg gtgagccgct cctgacgtta
     6241 gtcagttcag gctcatggcc cctggatcaa acgctcgcca gagatacccg cccatgaaca
     6301 aacaatcagg gatgacactg cttgaagtct tactggcgat gagtattttc accgctgttg
     6361 cgctgacttt aatgagcagt atgcaagggc aacgaactgc catcgagcgg atgcgtaatg
     6421 agacgctggc attgtggata gcggataacc agcttcagtc acaagattca ttcgacgagg
     6481 aaaacacgtc cagttctggc aaagagttaa taaatggcga agagtggaac tggcgcagcg
     6541 atatccactc aagcaaagac ggcacgcttc tggaacacac cataacggtg acattaccca
     6601 gcggtcagac aacctcgctt acgcgctatc aaagcatcaa taataaatcc gggcaggcac
     6661 aagatgatta atcgccagca gggtttcaca ttgctggaag tcatggcggc gctggcgatt
     6721 ttctcaatgc ttagcgtgct ggcgtttatg attttttctc aagcttccga gttgcatcaa
     6781 cgttcacaga aagaaatcca gcagttcaat cagctacagc gcaccatcac cattcttgat
     6841 aacgatctcc tgcaactggt cgcccggcgg aatcggagta cggacaaaat catggtgttg
     6901 ggtgaagaag cgatttttac cacgcaaagt cgcgatccgc tggcccctct cagtgaagca
     6961 caaaccttac tcactgttca ctggtatctg cgaaatcata cgctctaccg tgctgttcgt
     7021 acttctgtgg atggcaggaa ggatcaaccc gcccaggcga tgctggaaca tgtcgagagc
     7081 tttcttctgg aaagtaacag cggagaaagc caggagcttc ctctctcagt cacgttacat
     7141 ctgaagactc agcaatatgg ggcgttacag cgccgttttg cattacctga gcagttagcg
     7201 agagaagagt cgccagctca gacgcaggcc gggaataata accatgaata atgaacaacg
     7261 cggcgtagca ctgctcattg tactcatgtt actggcgctc atggctgctc tggctgcaga
     7321 catgacgatc agttttcacg gccagcttca tcgaacccgc caggtaaacc atcatttgca
     7381 acggcaatat gatattgagc tggcggagaa acttgcgctt gccagcctca ccctggacgt
     7441 taaagataac gacagacaaa ccacgttgca gcagtactgg gcacaaccgc aacagttgca
     7501 gctggaaaat ggcaatacag tgaagtggca gttacgcgat gcccagcact gttttaatct
     7561 caatgcctta gcaaaaatct ctgacgcccc gctggcttcc cctgatttcc ccgcccaggt
     7621 attcagtgcc ctgctgatta acgccggtat cgatcgcgga aacaccgatg agatcgtgca
     7681 atcaattgct gactatattg atgccgatga ttcgccacgc tttcacggcg cagaagataa
     7741 cttttaccaa agccaaacgc cgcctcgcca tagcgccaat cagatgcttt ttctgaccgg
     7801 tgaattgcgt cagataaaag gcataaccga aaacatctat cagcggctta tcccgtatgt
     7861 ctgcgttctc ccgacctcgg aacttagcat caatcttaat atgctaacgg agaacgatat
     7921 tccgctcttc agagcgttat tcctcaataa catcacggat gctgacgctc gggtcttatt
     7981 acaaaaaagg cccggggaag gctggctaac cacagatgca tttctttact gggcgcagca
     8041 ggatttctcc ggcgtgaagc ccctggttgc tcaggtgaaa gggcatctct ttccctatag
     8101 ccgctatttt acgctgagta cggaaagtat cagcgacgaa caatcccagg gatggcaaag
     8161 ccatattttt ttcaaccgta aacagcagag cgcccaaatc tatcggcgta cgctgcaact
     8221 ctactaatca taaggatcgt gatgcctgaa tcgttgatgg taatacgttc tttctcgacg
     8281 ctgcgtaagc attgggaatg gatgacgttc tccgcagata gcgtatcttc ggtacatacg
     8341 ctcacagacg atcttccgct ggagtcgctg gccgatcagc ccggtgctgg aaatgttcac
     8401 cttttgatcc cacctgaggg tcttttatat cgttccctca cgctgcccaa cgccaaatac
     8461 aaactcaccg cgcaaacatt acagtggttg gcggaggaga cattacccga cgccagccaa
     8521 aattggcact ggacggttgt cgataaacaa aacgaaagtg tcgaggtgat aggcattaag
     8581 tcggagaaac tcagtcgtta tcttgagcgt ttacatactg ccggactcaa cgtgacgcgg
     8641 gttttgccag atggctgcta tcttccctgg gaagtagata gctggactct ggttaaccag
     8701 caaacaagct ggcttattcg cagtgccgca catgccttca acgaactgga tgaacactgg
     8761 ttacagcatc ttgctaacca gtttccgcca gagaatatgc gttgttatgg tgtagcgcct
     8821 cacggcgttg cagcggccaa tccacttatc cagcatcctg aaataccatc actgagtttg
     8881 tattctgccg atattgcatt ccagcgctat gacatgctgc atggtgtttt tcgtaaacag
     8941 aaaactgtaa gcaaatcggg aaaatggctc gcccgcctgg cagtaagctg tctcgttctc
     9001 gccattcttt cttttgttgg cagccggggc atcgcgttct ggcaaacgct taagattgaa
     9061 gatcaacttc agcaacaaca gcaagaaacc tggcaacgtt atttcccgca gatcaaacac
     9121 acccacaact ttcgttttta ctttaagcag caactcgctc agcaatatcc tgaagctgta
     9181 ccgttgctct atcatttaca aacacttctg cttgaacacc ctgaactgca gttaatggag
     9241 gcgaactata gccagaggca aaagtcgctg accttaaaaa tgagtgctaa gagtgaagcc
     9301 aatatcgatc gtttctgtga gttaacacag tcctggcttc cgatggaaaa aacagaaaaa
     9361 gatccggtca gtggtgtatg gacagtaagg aactcaggga aatgattaaa tcatggtggg
     9421 cagaaaaatc cacatcggaa aaacagatcg ttgcggcatt ggcggttctc tcgctcggcg
     9481 ttttttgttg gcttgcggtg ataaaaccga tagataccta cattgaagag catcagtccc
     9541 acgcgcaaaa aattaagaag gacatcaaat ggatgcagga tcaagcgagc acccatggtt
     9601 tactcggtca tccagcgctt acgcagccca tcaaaaatat ccttctcgaa gaagcaaaac
     9661 gtgagaattt gactatcacg ctggaaaacg gtccggacaa tactctgaca attaaccctg
     9721 tcacagctcc cctcgaaaac gtctcccgct ggctcactac ggcacagata acatacggta
     9781 ttgttattga agaccttcag tttactcttg ccggaaacga agagatcacc ctcaagcatt
     9841 tgtccttcag ggagcaacaa taatgacaat gctactgccg ctcttcattc tggttggttt
     9901 tattgcaggt tattttgtta atgtcatggc ctatcacctc tcgcctttgg aggataaaac
     9961 ggcgttaacg tttcgccagg tattggttca tttctggcaa aaaaaatatg cctggcatga
    10021 tacagtgccc ctgatacttt gcgttgctgc tgctatcgcc tgcgccctgg cacctttcac
    10081 gcccattgtg actggcgcac tctttctcta tttctgtttc gcactcacgc tcagtgttat
    10141 tgattttcgc actcagctcc tgcccgacaa actcacctta ccgctgctct ggcttggctt
    10201 ggtatttaat gcgcagtctg gattaattga tttacatgat gcggtttatg gcgcggtcgc
    10261 tggctatggg gtgctgtggt gtgtttactg gggcgtctgg ttagt
//
LOCUS       DAZFFE010000139        10088 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFFE010000139 DAZFFE010000000
VERSION     DAZFFE010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.139"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..128)
                     /locus_tag="ACHJ69_004210"
     CDS             complement(<1..128)
                     /locus_tag="ACHJ69_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO5017067.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHW"
     gene            complement(209..412)
                     /locus_tag="ACHJ69_004211"
     CDS             complement(209..412)
                     /locus_tag="ACHJ69_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO5017068.1"
                     /translation="MNTTIALTLTVFLNTGEPVDMVIDIYGSMKECMAAAAEQKIPGN
                     CYPVDKVILMDNNEIPAGLKTAP"
     gene            complement(409..597)
                     /gene="dicB"
                     /locus_tag="ACHJ69_004212"
     CDS             complement(409..597)
                     /gene="dicB"
                     /locus_tag="ACHJ69_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO5017069.1"
                     /translation="METLLPNVNTSEGCFEIGVRISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPMQKGYWQ"
     gene            1164..1410
                     /locus_tag="ACHJ69_004213"
                     /pseudo
     CDS             1164..1410
                     /locus_tag="ACHJ69_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1388..1795)
                     /locus_tag="ACHJ69_004214"
     CDS             complement(1388..1795)
                     /locus_tag="ACHJ69_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001359738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017070.1"
                     /translation="MEFKDLPKEIQIIAATTLGDSLVKIDPAYTKKETIDNMVRNVRN
                     AFSGLYGSDNQKQKNDIDERVISVCLNGHVISAIRTETATVFDYLCMIQSLADVLLKS
                     KDLENDANLQGRTIAHPYAHTLGSVDIKDPTNL"
     gene            complement(1818..2036)
                     /gene="ydfC"
                     /locus_tag="ACHJ69_004215"
     CDS             complement(1818..2036)
                     /gene="ydfC"
                     /locus_tag="ACHJ69_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO5017071.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKIKAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(2066..2194)
                     /gene="ydfB"
                     /locus_tag="ACHJ69_004216"
     CDS             complement(2066..2194)
                     /gene="ydfB"
                     /locus_tag="ACHJ69_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO5017072.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSPAKSNG"
     gene            complement(2196..2351)
                     /gene="ydfA"
                     /locus_tag="ACHJ69_004217"
     CDS             complement(2196..2351)
                     /gene="ydfA"
                     /locus_tag="ACHJ69_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO5017073.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTESGARRW
                     LERDSGG"
     gene            complement(2518..2925)
                     /gene="dicA"
                     /locus_tag="ACHJ69_004218"
     CDS             complement(2518..2925)
                     /gene="dicA"
                     /locus_tag="ACHJ69_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5017074.1"
                     /translation="MDTKNLTIGERIRYRRKNLKYTQRSLAKALKISHVSVSQWERDD
                     SEPTGKNLFALSKVLQCSPTWILFGDEDKQPSPPIEEPVALSPKEHELLELFNALPES
                     EQDAQLTEMRARVKNFNKLFEELLKARQRTNKR"
     gene            3009..3239
                     /gene="dicC"
                     /locus_tag="ACHJ69_004219"
     CDS             3009..3239
                     /gene="dicC"
                     /locus_tag="ACHJ69_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicB transcriptional regulator DicC"
                     /protein_id="HFO5017075.1"
                     /translation="MLKTDALLYFGSKTKLAQAAGIRLASLYSWKGELVPEGRAMRLQ
                     EASGGELQYDPKVYDEYRKAKRAGRLNNENHP"
     gene            3223..3744
                     /locus_tag="ACHJ69_004220"
     CDS             3223..3744
                     /locus_tag="ACHJ69_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO5017076.1"
                     /translation="MKITPEQVCEALDAWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRKKFTALIPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYESSFC"
     gene            3725..4690
                     /locus_tag="ACHJ69_004221"
     CDS             3725..4690
                     /locus_tag="ACHJ69_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017077.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLMRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKVSESIGSKCAAPSGQNDTMEEVKMKRSIGSKRLNVIGSKWPDDLTENTT
                     EITTENKNTSRPEASQPDPQTVEQDFLTRHPDAVVFSAKKRQWGNQEDLACAQWIWGR
                     IVSLYEQAASDDGEISRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRAQRDPFWV
                     KNIMSPSKLRGKWDELVIRLGRSSVQRCVNHISEPDTEIPPGFRG"
     gene            4696..5442
                     /locus_tag="ACHJ69_004222"
     CDS             4696..5442
                     /locus_tag="ACHJ69_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5017078.1"
                     /translation="MKNIAAGGVLERIRKLTPQHVIAPYRTVDEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNHLLAKGRSVIVITVADVMLALHNSYDNKNSGEKFLQG
                     LCDVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASIRSVGMLTNLNHVAMSTLLGERV
                     MDRMVMNGGRWVNFNWESWRSNVRHLRVVK"
     gene            5457..5873
                     /locus_tag="ACHJ69_004223"
     CDS             5457..5873
                     /locus_tag="ACHJ69_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO5017079.1"
                     /translation="MVKVFTPEQREEVKARIVELVRRDGRKTRKQLENETGATRHLIE
                     VLAKELVDSGVVYGSGHGIFPSEQVRKDWIKAHKEMSKGAAKKKSDPGLIYSLPDGEI
                     RHYDRRQNIICLECRKSKVMQRVLAFYQGNFQEVMA"
     gene            5870..6175
                     /locus_tag="ACHJ69_004224"
     CDS             5870..6175
                     /locus_tag="ACHJ69_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO5017080.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKAEEELKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLKGWQRSAGAKAELALAEKLGHAVIFQETASVQD"
     gene            6162..6617
                     /locus_tag="ACHJ69_004225"
     CDS             6162..6617
                     /locus_tag="ACHJ69_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5017081.1"
                     /translation="MCKTNYQALRERYSPIQVPECSVCGDEMSIQRIFSRAHIVYACT
                     GEGDDGYFKTGRTFADEHYLKSRVTVVDVSDPDVLALLKELEVKDKRIAELTDALTQM
                     INAHKTTIRFGHERITECGGDCDSPEKMISENPDIRMAEAVLRAGIKTE"
     gene            6714..7130
                     /locus_tag="ACHJ69_004226"
     CDS             6714..7130
                     /locus_tag="ACHJ69_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017082.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIALASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMPVKRPAD"
     gene            7781..7936
                     /gene="hokD"
                     /locus_tag="ACHJ69_004227"
     CDS             7781..7936
                     /gene="hokD"
                     /locus_tag="ACHJ69_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO5017083.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            8152..8331
                     /locus_tag="ACHJ69_004228"
     CDS             8152..8331
                     /locus_tag="ACHJ69_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017084.1"
                     /translation="MSCPLKKHTRLSIPPRDKSVVAVPRPVIDENCAHREQVKNAFDF
                     GFSRYEKAMEELSKV"
     gene            8350..8835
                     /locus_tag="ACHJ69_004229"
     CDS             8350..8835
                     /locus_tag="ACHJ69_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFO5017085.1"
                     /translation="MAEIVEGVHYLTVDDLVEINHSLIELQTPDEPVGVLSPDNLSSS
                     QARPSMVRWYEQTNDMFVLASVLIESLIQNHPFANANKRTAMMAGYVFLLLNGYELTA
                     PGDEIVEMAEGLACKTYTREDLENWLCYWSRAYDSRELCKTSATIVLYETIKLKIEQQ
                     N"
     gene            9036..9296
                     /locus_tag="ACHJ69_004230"
     CDS             9036..9296
                     /locus_tag="ACHJ69_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017086.1"
                     /translation="MKIKELREIFSEDGLYTVRVENGAIVSHCRIKCLQSQQRKSGAA
                     LIHLVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFEDE"
     gene            9363..9641
                     /locus_tag="ACHJ69_004231"
     CDS             9363..9641
                     /locus_tag="ACHJ69_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017087.1"
                     /translation="MAQNSRLHNSDNSAAFASRHGRRSHAFKYDWYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRLWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            9643..>10088
                     /locus_tag="ACHJ69_004232"
     CDS             9643..>10088
                     /locus_tag="ACHJ69_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017088.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGVVPAVRQPLAEYKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGIGAMVLCWHCDNQLRDQTSESLGQLAHQNLSAWMIDV"
BASE COUNT         2756 a         2141 c         2547 g         2644 t
ORIGIN      
        1 tgccagtgaa attttgcgcg ggcgcgacgt tcatcaccgg cttcaatggc agtggctaca
       61 gcgactgcac cttcttcctt tattgcctgt tcgtccggaa tggcggcgca aataaagact
      121 ttactcatgt tgttttaacc tcattacaga tttaagggtg aacaaatctc tgccattgct
      181 ggcatataaa aatgaaaccg gatattaatt acggtgctgt tttaagtcct gccgggattt
      241 cgttattatc catgagaata actttatcga ccggatagca gttaccggga attttttgtt
      301 ctgctgcggc agccatgcat tctttcattg aaccgtaaat gtcaataacc atgtcaacag
      361 gctcgccagt attaagaaaa acagtcagag tgagggcaat agtagtattc attgccagta
      421 tcctttttgc atcgggcgta aacgtgccag cattgaaaga atgcatattt tatttaatag
      481 ctcccgttcg tgttttctct tattaatggc atcttcagta aatacagggt tactgattct
      541 gacaccaatt tcaaaacaac cttcagacgt attgacgttt ggtaataacg tttccattat
      601 cgcgtcctca acaatgaatt ttgtgatgcg gtgcctggtg cctccaggtg acgttaacca
      661 gttaataatt aacgccggat acagagaacc cacccataag aaccaatacg gaagtcaact
      721 ggccttttta actgttccgc gtgcgctgag ccgcattcac cgcatcacaa aattcacttt
      781 aaaaggggcg ggtatcatag gaaaaacaaa acggataccc gccaaaaggt aatcaacatg
      841 ggttgttgca gcggggttgt cactcaggcg tatggtcaac ctgacaaccc ggtggacatt
      901 tgcgaggaac ggagaacccc cgccatactt accgccgcgc catttcgcgg agtgccacaa
      961 ccggaagcgc acggtcgacg aaaatttaac gacaggctat ctatgaacca gcaacctcgc
     1021 cgtgcgcttt cgcgttatgc tctgactttt cagagaaata tcctttcagt aaactgtcag
     1081 tgccggatgc tcacccgtgt ccggcgcaca cactccacct cacccgtgga gaactcctta
     1141 attaccaacc ctcaggaggg tgaatgttaa aatcaacgct tattgctaaa tgcctttatc
     1201 aaaatcgcat ggtaagcagc atttcaatag gcgaatctgt agttaaaagt attttcgaag
     1261 agtactttcg aagagtactt ttctgggcgt gattttaata aatggaatac caaattaccg
     1321 ccagcagttt caacgcgtat tctgaaagca actgaaagag caagtacaat tcgcgttaac
     1381 tatttcatta aagatttgtg ggatctttga tatccacaga gcctaaagta tgtgcatatg
     1441 gatgtgctat tgtgcgcccc tgtaaatttg catcgttttc taaatctttt gatttcaaca
     1501 gaacatcagc aagactctga atcatgcaga gataatcaaa aacagttgcc gtttctgttc
     1561 tgattgctga aataacatga ccattcaggc aaacagaaat tacccgttca tcaatatcgt
     1621 ttttctgttt ttgattatca gaaccatata gccctgaaaa agcattgcgc acattacgaa
     1681 ccatattatc gatggtttct tttttggtgt atgccgggtc aattttcacc agactatcac
     1741 cgagtgtcgt tgcagcaatt atctggattt cttttggtaa atctttaaat tccattatta
     1801 gcctcgttgg ttaaccatta acgtgggtat gtaatcattc tggcaatgct taatgccgct
     1861 gctttttcca gattggtgat atcctgctcc agagcggaca gattttcagc ctgcttagcc
     1921 ctggcttcat tggcccattt caggtcctgc gcagccttaa ttttctggtg catccactca
     1981 taaagttcat catcggtata gtctggcgcg atgatgacgg gttctcgttt ctgcatactg
     2041 attcctcgcg gtgctgcttc gcttatcagc cgttagattt tgccgggctg gaaagcgcct
     2101 gtttaaattc gctgaagctg agggcttctt cgccttcggc aaggccttcg aagtattctt
     2161 cgtaagcctt ttccatgatt gtgtcgaaat ccatatcacc cacctgaatc tctttccagc
     2221 cagcgacgcg ctccagattc ggttttaaac gttttgcttt tggtatacgt cattgcggtg
     2281 aacgtgccgt cctggtttgg aaacacgccg tacaccagag attcgttgtt gccaagatcg
     2341 atagtatcca tgctgacctc atttcccctt aacgccgggg tagcggaact gtttgctgag
     2401 aacaccgtgc ggtgtcttga tgcaagcaag attagccatg actaacaatt tggtcaagca
     2461 tttttgtttg tcatgactaa cattttgggc aaccaaaaag ataacgcatt gattgcgtta
     2521 tcttttgttt gttcgttgac gggcttttaa taactcttca aagagtttgt tgaaattttt
     2581 tactcgggcg cgcatctcgg tgagctgagc atcctgttct gattcaggca atgcattaaa
     2641 aagctcaagg agctcatgtt ctttggggga tagagcaact ggctcctcaa taggtggtga
     2701 tggctgcttg tcttcatcgc caaatagaat ccatgttggc gagcactgca gtactttgct
     2761 gagggcaaaa agattctttc ctgtaggttc gctatcatcc cgctcccatt gtgaaaccga
     2821 tacgtgagaa attttcaggg ctttagcaag agacctttgg gtgtatttga ggttttttcg
     2881 gcgatatcta atgcgttcgc caatggttaa attttttgta tccatagtta gctaatgcta
     2941 aatcttattg actatgtttt tgttaacatc tattttgtta gttatggcta acaattaagg
     3001 tgctttaaat gcttaaaact gacgcacttt tgtatttcgg ttcaaaaaca aaacttgcac
     3061 aagctgctgg tattcgtttg gcttcgcttt atagctggaa aggggagcta gtacctgaag
     3121 gtcgcgcgat gcgcctgcaa gaggcatccg gcggggaact tcagtacgac cccaaagttt
     3181 atgacgaata tcgtaaggca aagcgggcgg ggcggttgaa caatgaaaat cacccctgaa
     3241 caggtttgtg aggctctgga tgcctgggtg tgccgaccag gaatgacaca ggagcaggcg
     3301 acgatattaa tcacggaagc attctgggct ctgaaagaac gcccgaacat cgatgttcaa
     3361 cgcgtcacgt ttaatgatgg cgaggttgat caacgggcgc tgggcgttaa ccgggtgaag
     3421 atattcgaac gctggaaagc tatcgacacc agggataagc ggaaaaaatt cacggcgctg
     3481 attccggcaa ttatggaggc tatccggatt aatgatttca ggttgtatcg tgaaattagt
     3541 gacggaaaaa gcatcacgta catgatcgcc gggttaaaca aagaatatgg cgatgtggtg
     3601 gagtccgggc tgctttttgc agatccagct gtagtggatc gtgaaactga cgaacttata
     3661 gaaaaagcca ttgctttcaa acttgcgtat cgacagcaat accaacaaaa agctggatgg
     3721 aattatgagt cttctttttg ctgaacgccc actggttata aacacgcagc tggcaatgaa
     3781 aattggctta aacgaagcca ttgttttgca acaactgcac tactggttga gagataccaa
     3841 ctccggcatg gaatgtgatg gtgttcgctg gatttataac acaacggaac aatggctgga
     3901 acagttcccg ttctggtcag agtcaacgtt aaagcgcgcg tttgcaagtc tgaaaacgct
     3961 ggggcttatg cgttgtgaaa agctcaataa atcaaagcgc gatatgacca atttctacac
     4021 gatcaactac gggagcgagc ttttagatgg tggcaaagtg agcgaatcca tcggttcaaa
     4081 atgcgccgct ccatcaggtc aaaatgacac gatggaagag gtcaaaatga aacgctccat
     4141 tggttcaaaa cggctcaatg tcatcgggtc aaaatggcct gatgatctta cagagaatac
     4201 aacagagatt actacagaga ataaaaacac ttctcgtccg gaagcttcgc aaccggaccc
     4261 gcagacggtt gaacaggatt ttttaacccg acaccctgac gcggttgtgt tcagtgcaaa
     4321 aaaacgccag tggggcaacc aggaagattt ggcgtgtgcg cagtggatct ggggacgaat
     4381 cgtgagtctt tacgagcagg ccgccagcga tgatggcgag atttcgcgac cgaaagaacc
     4441 caactggacc gcatgggcca acgacgtgcg cacaatgcgg atgctggatg gcagaactca
     4501 cagacaaatt tgtgaaatgt ttggtcgggc gcagcgggat ccattctggg taaaaaatat
     4561 catgagtccg tcaaagcttc gcggaaaatg ggatgaactg gttatccgcc tggggcgttc
     4621 gtctgtacag cgttgtgtga atcatatttc tgagccggat accgaaattc cgccggggtt
     4681 cagggggtaa cggccatgaa aaatatcgcg gcaggtggtg ttcttgagcg tatccgtaag
     4741 ctgaccccgc agcatgtaat cgcgccgtac cggacagtgg atgagtggcg cgagtggcaa
     4801 ctggctgaag ggcgaaaacg tagcgaggag atcaaccgcc agaatcgcca gttgcgggtg
     4861 gaaaaaatcc tgaatcgttc gggcatccag cctctgcaca gcaaatgctc gtttgcgaat
     4921 tatcaggtgc agaacgacgg gcaaaaacat gcgctgagcc aggcaaaatc catcgctgac
     4981 gaactgatga ccgggtgcac gaattttgtg ttcagcggta agccgggtac cggaaagaac
     5041 caccttgcag ccgccattgg caatcatctt ctggcgaaag gtcgcagcgt gattgtgata
     5101 acggtggctg atgtgatgct ggcgttacac aacagctacg acaacaaaaa ctcaggcgaa
     5161 aaatttttac aggggttgtg tgatgttgac ctgcttgtcc tggatgaaat cggaatgcag
     5221 cgggatacgc gcaacgagca ggtcacactg aaccagatag tcgaccgcag aacggcttcg
     5281 attcgtagtg tcggaatgct gacgaacctg aaccacgtag cgatgagtac gcttcttggc
     5341 gagcgtgtga tggaccgcat ggtcatgaac ggtggtcgct gggtgaattt taactgggag
     5401 agctggcgtt cgaatgtcag acacctgagg gttgtgaagt aatttcagga ggacttatgg
     5461 taaaagtttt tactcccgaa caacgggaag aagtaaaggc gcgtattgtg gaactggtac
     5521 gcagagatgg taggaaaacg cgtaaacaac tggaaaatga aacaggggcg acgagacatc
     5581 tgattgaagt tctggcgaaa gaactggtag acagtggtgt tgtatatggt tcagggcatg
     5641 gaatattccc ttctgagcag gtacgtaaag actggataaa agcccataaa gagatgtcta
     5701 aaggtgcagc gaaaaagaag agcgaccctg gcctgattta ttcattacca gatggagaga
     5761 tacgccacta cgacaggcgt cagaacataa tctgtctcga gtgccggaaa agcaaggtta
     5821 tgcagcgtgt gctggcgttt tatcagggta attttcagga ggtgatggcg tgagggtgag
     5881 ggtttatatc gccggtccaa tgacggggta tgaaaatttc aaccgtgagg cgtttcacaa
     5941 ggcagaagag gaactgaaac gggaagggca taccgtttta aatccggctg tgcttccgga
     6001 tgggctgaca cagccgcatt acatggatat ttgcatggca atgattcgtt gtgtggatgc
     6061 gatttacatg ctgaaaggct ggcagcggtc agcaggcgct aaggcagaac tggcgctggc
     6121 ggagaaactg gggcatgcgg tgatttttca ggagacagcc agtgtgcaag actaattacc
     6181 aggcattacg tgaacgttat tcaccaattc aggtgccgga atgttctgtt tgtggcgatg
     6241 aaatgtcgat acagcgcata ttttccagag cgcatattgt gtatgcctgc acaggtgagg
     6301 gggatgatgg atattttaaa actggtcgaa cttttgcgga tgaacattac ctgaaatcgc
     6361 gcgtaaccgt cgttgatgtt agcgatcctg acgtactggc tttactgaaa gagctggaag
     6421 ttaaagacaa acgcattgca gaattgacag acgcgcttac acaaatgatt aatgcgcaca
     6481 aaaccacaat tcgttttggt catgaacgca taactgaatg tggtggtgat tgcgactcgc
     6541 cggaaaagat gatttcagaa aatccggata tcagaatggc agaggctgtt ttgagagcag
     6601 gaataaaaac tgaataatta aatttagcac agcaaataaa atttaatcct taaccggagg
     6661 gatttctgca ccctcagaac atcaggaggc cgcctgaaag ggcggtaatg aaaatgactg
     6721 aattaacaaa agaacaatta atcgaagaag ccaaattaaa aatagcgatt acgaaatgcc
     6781 accccaattc agggatggcg cgagtagagg gcgagttatt caaaattgca ctggcatcgc
     6841 tggaagcaga gccgatagcg tggcgatatc gctacgtgaa aaaaggtgtt atggactctc
     6901 agggggagtt gtgggttggt gactggaaat atgtaccgaa aaaagaggat tgtaacgaca
     6961 ggccgaacta tgaaattcag gccttattca ctgccccacc agtcccggtt acatcagaag
     7021 aactggttaa agctgtgcac ttttatgaac aactaaaacg cgaaaatcca ccagcatccg
     7081 gcaaccagat taatgggtta actatgccgg ttaaacgacc agccgactga aaaacggaaa
     7141 cctgattaca gattcccaga taaggcaatg agctacctgg cgtagagtgg gctgataagt
     7201 atggggaatg ttttacgatg aatacttaga ctaaagagtt tgtaacgcta tgtaagtgat
     7261 tttttctggt ttagatattt atatgtccgg ccaaattgag gtgtgtttaa atgtaattgc
     7321 acattgattg tagggggaat aatgaaaaac gcattgcagt ttttgtttgt tgcgttctgg
     7381 ttgttcgcat catgtatgcc catcatcttc acagcaaggt atatggaaaa aattgatgtt
     7441 ttgatattaa tgtttggaca tataaatgcc ctttttttag gggtgttcat ggcggtcacg
     7501 tgcattgaat actggcggta aatacagcga acgctattgg tttagttgga tatttactgt
     7561 gccggacaaa aacggtttgc agggaaatct tagttaagta gaatgactgt gggtgcttga
     7621 ggctatctgt ctcaggcatg aacaccaaaa ggcagctaga gaaaagcccc agttaacatt
     7681 acgcgtcctg caagacgctt aacattaatc tgaggctcaa tccatgctga acacatgtag
     7741 gttagcctct tacgtgccga aaggcaagga gaagcaggct atgaagcagc aaaaggcgat
     7801 gttaatcgcc cttatcgtca tctgtttaac cgtcatagtg acggcactgg taacgaggaa
     7861 agacctctgc gaggtacgaa ttcgaaccgg ccagacggag gtcgctgtct tcacagctta
     7921 cgaacctgag gagtaagaga cccagcgggg gagaaatccc tcgccgcctc tgatgtgtca
     7981 ggcatcctca acgcacccgc acttaacccg cttcggcggg tttttcgtcg cattatgagg
     8041 ttgtaatttt agctaccatg agactatcct aaggatctca aacagatcta ttttgtatca
     8101 aatttggtgc atggctttgc caataatcgg aaaacaaaag gactcattag tatgagctgc
     8161 ccactcaaaa aacacacgcg gttgagtatt ccgcctcgcg ataaaagcgt tgtggcagtc
     8221 cctcgcccag tgattgatga aaactgcgca catcgtgaac aagtgaaaaa tgcttttgat
     8281 ttcggttttt ctcgttatga gaaagccatg gaagaacttt caaaagtgta atgatgggta
     8341 ttgtactcta tggctgagat tgttgaagga gtgcattacc ttacggttga tgatcttgtt
     8401 gaaatcaatc attccctaat tgaattacag acgccagatg agcccgttgg tgttctgagt
     8461 ccagataact taagttcttc tcaggcccgt cccagcatgg ttcgatggta tgaacagact
     8521 aatgacatgt ttgtactggc atcggtattg attgaaagtc tgattcagaa tcatccgttt
     8581 gctaatgcga acaaacgaac agctatgatg gctggttacg tcttcttgtt gttgaatggc
     8641 tatgagttaa cagcaccagg cgatgaaatc gtggaaatgg cagagggact ggcctgcaaa
     8701 acctatactc gagaagatct cgagaactgg ttgtgttatt ggtctcgcgc gtatgacagc
     8761 cgggaattat gtaaaacaag cgcaactatt gttttgtatg aaactatcaa gcttaaaata
     8821 gaacagcaaa actaaaggtg cttccaatga aaacccgctt cggcgggttt tttattttaa
     8881 tggactaaat tttagccaat tcttctattt aaccgaccag ggtttgttga ttagctggta
     8941 tgcctgacta gaatcgatgc acttaagtag cgcgcaggga gaagagggat ggaccccaaa
     9001 caggggagtg ctatttatct ggaaggattc tgttgatgaa aatcaaagaa ttacgtgaaa
     9061 tttttagtga agatggcctc tatactgtgc gcgttgagaa tggcgcaatt gtcagccact
     9121 gccgtattaa atgtttacag tctcaacaaa ggaagagtgg agctgcgtta attcatttgg
     9181 tggatgggct tgtgacggat ggttttattt tgcgtgcaaa tgaatttgtc acatcgttgc
     9241 cgtctctgaa agaagctggg attaaggctg gtttttctgc ttttgaagat gagtgaattc
     9301 atctacaatt cagcgcaggg ctgaacccct gttgagtaac actgtgccac cggagaaagc
     9361 cgatggcgca aaattccaga ctacacaatt ctgataattc agccgccttt gccagcaggc
     9421 acgggcggcg ctctcatgca ttcaaatatg actggtatca gcacgatccc tgcaccgaag
     9481 aacaggctga atggctgatt cagcgctacc gcagacacgg atacgagatt aagaaagccc
     9541 tcagcctcga ttatcgtctc tggataatct acgtcagact cccttattcc gaacgcccac
     9601 cgcgtccgtc ccgcacattc cagcaacgca tctggaggta acgtgcgggt attacttcga
     9661 cctgttccgg taccggaact tgggctggtg gtgctaaaac cgggccgtga atccatgcag
     9721 gtatttcata accctcgagt tctggtggag ccggaaccga aaagcatgcg cggtctgccg
     9781 tccggagtcg ttcctgccgt tcgccagcca ttggcggagt ataaatcact actgccattt
     9841 ttcagcgatg agcgggtgat tcgtgctgct ggcggtgctg gtgcgctgtc tgactggctg
     9901 ttacgccacg ttaaatcctg ccagtggcca cacggcgatt atcatcacag cgaaaccgtc
     9961 atacatcgtt atggtatcgg cgcaatggtg ttgtgctggc actgcgacaa ccagctgcgt
    10021 gaccagacct ccgaatcact cgggcaactt gctcatcaaa acctgtcagc atggatgatt
    10081 gacgtcat
//
LOCUS       DAZFFE010000140         9889 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZFFE010000140 DAZFFE010000000
VERSION     DAZFFE010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.140"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..807
                     /locus_tag="ACHJ69_004233"
     CDS             <1..807
                     /locus_tag="ACHJ69_004233"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO5017089.1"
                     /translation="VNLNIEINQTLEKMSQTLFKSWFVDFDPVIDNALDAGNPIPEAL
                     QARAELRQKVRNSTDFKPLPAEIRSLFPSEFEETELGWVPGGWKTNRLENILELAYGK
                     ALKKTERIEGDYPVYGSGGVDGSHNEFLVKGPGIIVGRKGTVGSLYWENKDFYPIDTV
                     FYVKPKKYFSLVYCYQLLKTLGLENMNTDAAVPGLNRNNAYRLDVITPTQTIIAQYTN
                     IVQTFRYKMDSNNNEIDNLTNLRDTLLPKLISGELSLDDIPDLNTDTEAA"
     gene            902..4165
                     /locus_tag="ACHJ69_004234"
     CDS             902..4165
                     /locus_tag="ACHJ69_004234"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFO5017090.1"
                     /translation="MSLSFSEAKLEQAIIELLQDQGYQHLIGDNVPRSSLDQVIIEDD
                     LRHYLAARYQPDGITEEEIQRLIKQFTTLPASDLYESNKTFCRWLANGFLFKRDDRQQ
                     KDLYIELLDTRHLPAALRQIFDAEDVPLQQVAELPPSYINPPLNRIKIVNQLKISGKD
                     NQTRIPDGILYINGLPLVVFEFKSAVREQEASIGDAWKQLCKRYRRDIPQLFIYNALC
                     IISDGVNNRMGNLFAPYEYFYSWRKVTGNENREQDGIPSLHSMIQGLFHPVRLLDVIK
                     NFICFPDKAKHEVKICCRYPQYYAARKLYYSIKQARKPFGSGKGGTYFGATGCGKSYT
                     MQFLTRLLMKSVEFASPTIVLITDRTDLDDQLSAQMCNAKNYIGDDTIVPVTSREDLR
                     NQLAGRNSGGVFLTTIHKFTEDTELLSERSNIICISDEAHRSQVNLDQKVIVDKESGK
                     VRKTYGFAKYLHDSLPNATYVGFTGTPIDATLDVFGEVIDSYTMTESVQDEITVRIVY
                     EGRAAKVILDSSKLEEVEKYYEECANAGTNEWQIDESKKATATMNAVLGDEDRLKALA
                     EDFAKHYEKRVAEGSTVIGKAMFVCASREIAWDFYRQLKAIRPAWFEVKQAPDGVVLT
                     EQEQKELPPSEMVKMVMTRGKDDDEALYDLLGTKEYRKELDKQFKNAKSNFKIAIVVD
                     MWLTGFDVPELDTIYIDKPLQKHNLIQTISRVNRKLEGKSKGLVVDYIGIKSQMNQAL
                     AMYSRIDATNFEDIQQSVIEVKNHLDLLGQVFYEFDSRDYFSGEPQAQLSCLNRAAEF
                     VLRTQKVERRFMGLVKRMKAAYDVCCGSEALSQTERDYIHYYLAVRSIVFKLTKGDAP
                     DVTQMNARVREMISEALKADGVEEIYFLGDKKAESIDIFDEDYLARINKIKLPATKIQ
                     LLQKLLEKAISDFKKVNQLQGINFTRRFQAIIDRYNERREDDVLNGEEFDTFSQEMTD
                     IIYDIKTEMGTWADLGIDIEEKAFFDILAHMRDKYQFTYDDEKMLSLAKEMKSVVDNT
                     SKYPDWSKRDDIKAKLKVELILLLHKHKFPPVANDDVYMGVLAQAENFKEHHMSALN"
     gene            4380..4718
                     /gene="symE"
                     /locus_tag="ACHJ69_004235"
     CDS             4380..4718
                     /gene="symE"
                     /locus_tag="ACHJ69_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFO5017091.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLETAGFATGTAVDVKVMEGCIVLTAQPPAAEESKLMQSLRQVCKLSARKQKQVQEF
                     IGVISSKTPR"
     gene            complement(4761..4925)
                     /locus_tag="ACHJ69_004236"
     CDS             complement(4761..4925)
                     /locus_tag="ACHJ69_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO5017092.1"
                     /translation="MEFHENKAKAPFTGIVQLWQAVRTWWLKKQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            5102..5410
                     /locus_tag="ACHJ69_004237"
                     /pseudo
     CDS             5102..5410
                     /locus_tag="ACHJ69_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5408..6328)
                     /locus_tag="ACHJ69_004238"
     CDS             complement(5408..6328)
                     /locus_tag="ACHJ69_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO5017093.1"
                     /translation="MTNFTTSTPHDALFKSFLTHSDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRQLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFNEFISEITRRLPQQRERIMTIAERIHNDGYIKGEQRILRLFLQNGADPEW
                     IQKITGLSAEQMQALEQPLPDREHDSWLEY"
     gene            6513..7793
                     /gene="yjiN"
                     /locus_tag="ACHJ69_004239"
     CDS             6513..7793
                     /gene="yjiN"
                     /locus_tag="ACHJ69_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO5017094.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPVISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTRNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIEKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNL"
     gene            7909..9060
                     /gene="yjiM"
                     /locus_tag="ACHJ69_004240"
     CDS             7909..9060
                     /gene="yjiM"
                     /locus_tag="ACHJ69_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO5017095.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPHPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVSQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            9070..9837
                     /gene="yjiL"
                     /locus_tag="ACHJ69_004241"
     CDS             9070..9837
                     /gene="yjiL"
                     /locus_tag="ACHJ69_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO5017096.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLEPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVIPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
BASE COUNT         2630 a         2373 c         2421 g         2465 t
ORIGIN      
        1 gttaatttaa atatagaaat taaccaaaca ctcgaaaaaa tgtcccaaac cctgttcaaa
       61 tcatggtttg tggattttga tccggtgatt gataacgcgc tggatgcagg aaatccaatc
      121 ccggaagccc tgcaagctcg tgccgaattg cgtcaaaaag tacgtaatag tacagatttt
      181 aaaccgcttc cggcggaaat tcgctcgctt ttcccaagtg aatttgaaga gacggagtta
      241 ggttgggtgc caggaggctg gaaaacaaac cgactggaaa atattttaga gctggcttat
      301 gggaaagcac tcaaaaaaac agaaagaatc gaaggagatt atcccgttta tggttctggt
      361 ggggtggatg gttcccataa tgagtttctt gttaaaggac ctggtattat tgttggaagg
      421 aaaggaactg ttggttcatt gtattgggag aacaaagatt tttacccaat tgatacagta
      481 ttttatgtaa aacctaaaaa gtacttctcg cttgtttact gctatcagct cttgaaaaca
      541 ctaggattag aaaatatgaa tactgatgcg gcagtccctg ggttgaacag aaacaatgct
      601 tatcgtcttg acgtcataac accaacacaa acaatcattg cccaatatac aaatatagta
      661 caaacgttca ggtataaaat ggattcaaat aataatgaga ttgacaatct aaccaacctc
      721 cgcgacaccc tgctcccgaa actcatctcc ggcgagctat ccctggacga tattccggat
      781 ctcaacaccg atacagaagc cgcataacgc attttgcccc tgtaaaatca ggggctttct
      841 ggtaaggttt tctactgata cgggaatgct taccagaaat tagccagggt tggagcgcga
      901 tatgagtctc tctttcagtg aagcaaaatt agaacaagcg atcattgaac tgttacagga
      961 tcagggatat caacacctga tcggcgataa tgtcccacgt tcaagtctcg atcaggtcat
     1021 tatcgaagac gatctccgtc attatttagc ggcacgctac cagcctgatg gcattactga
     1081 agaagagatt cagcgactga tcaaacagtt caccacgctt ccggcctccg atctttatga
     1141 aagcaacaaa acattttgtc gctggctggc aaatggtttt ctgttcaaac gcgacgatcg
     1201 gcaacaaaaa gatctctata ttgaattgct cgacacccgg catctacctg ccgcgctgcg
     1261 ccagatattt gacgccgaag atgtcccgtt gcaacaggtg gcagaactcc cgccctccta
     1321 tattaatccg ccgcttaacc ggattaagat tgttaaccag cttaaaatct ccggcaaaga
     1381 taatcagact cgtattcctg acggcattct ctatatcaac ggtctgccac tggttgtctt
     1441 tgaatttaaa agtgcggtac gcgagcagga agccagtatt ggcgatgcct ggaagcagct
     1501 ctgtaaacgc taccgccggg atattccaca actgtttatc tacaacgcgc tctgcattat
     1561 tagcgatggc gtaaataacc ggatgggcaa cctgtttgcg ccctatgaat acttttactc
     1621 atggcgcaaa gtcaccggta atgaaaaccg tgaacaggat ggaattccat cactgcactc
     1681 aatgattcag gggctgtttc atccggtacg tctgctggat gtgattaaaa actttatctg
     1741 cttcccggat aaagccaaac atgaagtaaa aatttgctgc cgatatccgc agtactatgc
     1801 cgcccgcaaa ctctattaca gcatcaagca agcacgtaaa cctttcggta gcggtaaagg
     1861 cggcacctac tttggcgcaa cgggctgtgg caaaagttac accatgcaat ttttaacgcg
     1921 tcttttgatg aagagcgtgg agtttgccag cccgaccatt gttttgatca ccgaccgtac
     1981 cgatctggac gatcagcttt ctgcgcaaat gtgcaacgcc aaaaattaca ttggcgatga
     2041 caccatcgtt cccgttacca gccgtgaaga tttgcgtaat caactggcgg gacgcaatag
     2101 tggcggtgta ttcctgacaa cgatccataa attcaccgaa gacaccgaac tcctttctga
     2161 acgcagcaat atcatttgca tctcggacga agcacatcgc agccaggtta acctcgacca
     2221 gaaagtcatc gtcgataaag aaagtggaaa agtgcgcaaa acgtatggct ttgcgaaata
     2281 cctgcacgat tcactgccaa acgccaccta tgttggcttt accggcacgc cgattgacgc
     2341 gacgctcgat gtcttcggtg aggtgatcga cagttacaca atgaccgaat ccgttcagga
     2401 tgagatcact gtacgcatcg tatacgaagg tcgtgcggct aaagtgatcc tggactccag
     2461 caaactggaa gaagtcgaaa agtattacga agagtgcgca aacgcaggca ccaatgagtg
     2521 gcaaatcgac gaaagcaaga aagccacggc taccatgaat gcggttctgg gtgatgaaga
     2581 tcggttaaaa gccctcgcgg aagattttgc taaacattat gaaaaacgcg tagccgaagg
     2641 ttccaccgta ataggcaaag ccatgtttgt ttgtgccagc cgtgaaattg cctgggattt
     2701 ctaccgccag cttaaagcta ttcgccctgc ctggtttgaa gtgaagcaag cacctgatgg
     2761 cgtcgtcctg acggaacagg aacaaaaaga gttaccgcct tctgaaatgg tgaaaatggt
     2821 catgacgcgc ggtaaagatg acgacgaggc gctttatgat ttactgggca cgaaagaata
     2881 tcgtaaagag ctggataagc agttcaaaaa cgctaaatcg aatttcaaaa ttgccattgt
     2941 cgttgatatg tggctgaccg gttttgatgt tcctgaactg gataccatct atattgataa
     3001 gcccttacaa aaacataacc ttatccagac tatttctcgc gttaaccgta agctggaagg
     3061 caaaagcaaa gggttagtgg tggactacat cggcattaaa agtcagatga accaggcact
     3121 ggcaatgtat tcccgcattg atgccaccaa ctttgaagat attcagcaat cggtaataga
     3181 agttaaaaac catctcgatt tgttggggca agtcttttat gaatttgaca gtcgggatta
     3241 ttttagtggt gagccacaag cgcaattatc ctgcctcaac cgcgcggcgg aatttgttct
     3301 gcgcactcag aaagttgaac gtcgttttat gggactggtt aaacgcatga aagccgccta
     3361 cgacgtctgc tgcggcagtg aagcactatc acagacagaa cgtgactata ttcactatta
     3421 tcttgctgtt cgttcaattg ttttcaaact gacgaaaggt gacgcaccgg atgttaccca
     3481 gatgaatgca cgcgttcgtg aaatgatttc agaagcgtta aaagctgatg gcgtagaaga
     3541 aatttatttt cttggcgaca aaaaagcgga atccatcgat atttttgacg aagattatct
     3601 ggcgcgaatt aacaagatca aacttccggc aacgaagatc cagttattac aaaaattact
     3661 ggaaaaagcg atcagcgact tcaagaaagt gaaccagttg caagggatta acttcacccg
     3721 ccgcttccag gcgattatag atcgttataa tgagcggcga gaagatgatg tactcaacgg
     3781 tgaagaattc gatacattca gtcaggaaat gaccgatatt atctatgata ttaaaaccga
     3841 aatgggcacc tgggccgatt taggtattga tattgaagaa aaagcctttt tcgacattct
     3901 tgctcatatg cgcgataaat atcagttcac ctatgacgat gaaaaaatgc tgtcactggc
     3961 aaaagagatg aaaagcgtgg ttgacaacac atcgaaatat cctgactgga gtaaacgcga
     4021 tgatattaaa gcgaaactga aagttgaact tattctgctt ctacacaagc ataagttccc
     4081 gccagtagcg aatgatgatg tttatatggg tgtgctggca caggcagaga attttaaaga
     4141 acatcatatg agtgctttaa attaattatg aaggccggat acactttgtt atccggccat
     4201 tctgaattaa cgttaatttc tcttccagat ctttccagta acgcttttat tattttctat
     4261 ttttagctgg actttccctg tatttactga tgatatgtcc aggtatttag cgcggtacgg
     4321 atgtgcgcca acacacccgc accgctcatc acaatcacta ttttcaagga atagcagtta
     4381 tgactgacac gcattctatt gcacaaccgt tcgaagcaga agtctccccg gcaaataacc
     4441 gtcaattaac cgtgagttat gcgagccgct acccggatta cagccgtatt cccgccatca
     4501 ctctgaaagg tcagtggctg gaaaccgccg gttttgccac tggcacggcg gtagatgtca
     4561 aagtgatgga aggctgtatt gtcctcaccg cccaaccacc cgccgccgaa gagagcaaac
     4621 tgatgcagtc actgcgccag gtgtgcaagc tgtcggcgcg taaacaaaag caggtgcagg
     4681 agtttattgg ggtgattagt agcaaaacgc cacgctaaga ttctctcccc ttccccacac
     4741 gggaagggga attttcctgg tcactccaca tcctccctgc gtaacccaat atccctcaac
     4801 cgctcatcac tcatctgctg taacacgcgc cgggtctgct ttttcagcca ccaggtcctc
     4861 accgcctgcc agagttgtac gatgccagta aacggcgctt tggctttgtt ttcgtgaaat
     4921 tccatctgct gcctcctcat ctttcagagg cttcatcatc cgccttttgg cagttcacaa
     4981 tacagattca aatattactt ttatttaaca tacagacaga ttaaaacagc atctgcatcc
     5041 ggtttttaag cctcatctgt accggttttt ctctctgtat ggtccaaaga aggaatacag
     5101 catgacgcgt tatcaacatc tggcgactct gcttgccgag cggattgagc aagggctgta
     5161 tcgtcacggg gagaaattgc cgtcggtgcg cagcttaagt caggagcacg gcgtcagcat
     5221 cagtaccgtg cagcaggcgt atcagatact ggagacgatg aagctcatca ctccgcagcc
     5281 gcgttcgggt tattttgtcg cgcaacgtaa agcccagccg ccagtaccgt cgatgacgcg
     5341 tccggtggaa attacccagt gggatcaggt gctggatatg ctggaggcac atagcgatag
     5401 ttccatatta gtactcgagc catgaatcgt gctcacgatc aggcaagggc tgctctaatg
     5461 cctgcatttg ctctgccgaa agtccggtaa tcttttgtat ccattcagga tctgccccat
     5521 tctgcaaaaa caatcgaaga atgcgctgtt ccccttttat atatccatca ttatgaattc
     5581 gctctgcaat cgtcattatt cgctcccttt gttgtggcag tcgacgggta atttcactga
     5641 taaactcatt aaaacgcgct tcatcaccag tcagtaaaat gtaatttaac agcgcagtta
     5701 tctggctgtc attagcacac tctgtaacca gtaatactac cagttggtca ataagcccca
     5761 tcagatcgcg ctggcgaata tgcttttgga tcaactccaa cagggcgact ctgcgatgct
     5821 gcacaatctc gtcgtctggc acgacagtga tatccaccag cgggaacgcg gcggtataaa
     5881 gcttccgtgc ggtagtcggg tcggcaaatt cgtccagcca gcacaaagac cagggataag
     5941 ggctacggct accgtgataa aacagcatcg ggatgaccag cggtagctgc cggcgtttat
     6001 catgttcgat atggcgctgc atcaccgcca tggaatatcg catcagacga aaggccatat
     6061 gaatatcctc acggctctga tgttcaatca ctacataaat atagccatcg ccttcacggg
     6121 tctttaccga ccacagaata tcggaatgta gcgcccgcaa tttttcatcg acaaagctgg
     6181 cggattccag ttttaagcta tcgagatcgc acagttcacg taaatctttg ggtaagtgga
     6241 tctccataaa atcccgcgcg gtgtcagagt gtgtgagaaa ggatttaaat aatgcgtcat
     6301 gcggcgtgct ggtcgtaaag tttgtcatgg cgatccgtca cctgtagata agatggccca
     6361 actttagcga ctaacaaagc cattcacacc tggctatttc ctattccccg aggcggtttc
     6421 caggctattt ttattcttct gcaaacgata catttttatg gaagatatct cgctttttgc
     6481 gttttcttac cgtcaggctc tattcttacg atatgaataa actcattgaa ctcagacgcg
     6541 ccaaaatgtt ggcgctctct ttactgctta tcgccgctgc tacctttgtc gttacgctgt
     6601 ttttgccgcc caatttttgg gtgagcggtg tgaaggcgat tgctgaagcg gcgatggtcg
     6661 gcgcgctggc ggactggttt gcggtggtgg cgctgtttcg ccgcgtgccg attccggtca
     6721 tttctcgtca tacggcgatt atcccgcgta ataaagaccg gattggcgaa aatctcggcc
     6781 agttcgtgca ggaaaaattt ctcgataccc aatcgctggt ggcattgatt cgacgtcacg
     6841 aaccggcgtt gttgattggc aactggttta gtcagccgga gaacgcccgc cgcgttggtc
     6901 agcatctgtt acagatcatg agcggttttc tcgaactgac cgatgatgcg cgtattcagc
     6961 gcctgcttaa gcgtgcggtc caccgggcga tcgataaggt cgatctttcc ggcaccagtg
     7021 cgttgatgct ggagagtatg accagaaacg atcgtcatca ggtgctgctc gatacgctga
     7081 tcgcacaact gatcgccctg cttcagcgcg ataaatcacg caagtttatt gcccagcaaa
     7141 ttgttcgctg gctggagagc gagcatccac tgaaagccaa aattttgccc actgaatggc
     7201 tgggcgaaca tagcgcggag ttggtttctg acgcggtgaa ttctttgctt gatgatatca
     7261 gccgcgatcg tgcgcatcag atccgccatg cgtttgatcg cgctaccttc gccctgatcg
     7321 agaaactgaa aaacgatccg gaaatggcag cgcgagccga tgccgtaaaa agctatctga
     7381 aagaagatga agcttttaac cgctatctca gtgaattgtg gggggattta cgggaatggc
     7441 tgaaagcgga tatcaacagt gaagattctc gtgtgaaaga acgtatcgcg cgggcggggc
     7501 aatggtttgg cgaaacgtta attgccgatg atgccttgcg ggcgtcgtta aatggtcatc
     7561 tcgaacaagc cgcacaccgc gttgcgcctg agttttccgc attcctgact cgccatatca
     7621 gcgacacggt aaaaagctgg gacgcacggg atatgtcgcg gcaaatcgag ttaaatatcg
     7681 gcaaagatct ccagtttatc cgtgtcaacg gtacgctggt tggcggttgt attggactta
     7741 ttttgtattt gctgtcgcag ctcccggcct tgttccccct cggcaattta tagaaatcca
     7801 tgaaaaacta agtggctaac cgcgagagag atcaaataaa ccacactaaa agaatggtaa
     7861 taatgtggcc tcttttcatt atctcccgtg gtacggggaa ggaaaatcat gtcacttgtc
     7921 accgatctac ccgccatttt cgatcagttc tctgaagctc gccagaaagg ctttctcacc
     7981 gtcatggatc tcaaggagcg cggcattccg ctggttggca cctactgcac ctttatgccg
     8041 caagagatcc cgatggcagc cggtgcggtt gtggtttcgc tatgttccac ctctgatgaa
     8101 accattgaag aagccgagaa agatctgccg cgcaacctct gcccgctgat taaaagcagc
     8161 tacggcttcg gcaaaaccga taaatgcccc tacttctact tttctgatct ggtggtcggt
     8221 gaaaccacct gcgacggcaa aaagaaaatg tatgaataca tggcggagtt taagcccgtt
     8281 catgtgatgc aactgccaaa cagcgttaag gacgatgcct cgcgtgcatt atggaaagcc
     8341 gagatgctgc gcttgcaaaa aacggtagaa gaacgttttg ggcacgagat tagcgaagat
     8401 gctctgcgcg atgccattgc gctgaaaaac cgcgaacgtc gcgcactggc caacttttat
     8461 catttggggc agttaaatcc tccggcgctt agcggcagcg acattctgaa agtagtctac
     8521 ggcgcaacct tccggttcga taaagaggcg ttgatcaatg aactggatgc aatgaccgcc
     8581 cgcgttcgtc agcagtggga agaaggccag cgactggacc cgcacccacg tattttaatc
     8641 accggctgcc caattggcgg cgcagcagag aaagtggtgc gcgcgattga agagaatggc
     8701 ggctgggttg tcggttatga aaactgcacc ggggcgaaag ccaccgagca atgcgtggcg
     8761 gaaacgggtg atgtgtacga cgcgctggcg gataaatatc tggcgattgg ctgctcctgt
     8821 gtttcgccga acgatcagcg cctgcaaatg ctcagccaga tggttgaaga atatcaggtc
     8881 gatggcgtag ttgatgtgat tttgcaggcg tgccatacct acgcggtgga atcgctggcg
     8941 attaaacgtc atgtgagcca gcagcacaac attccttata tcgctattga aacagactac
     9001 tccacctcgg atgtcgggca gctcagcacc cgtgtcgcgg cctttattga gatgctgtaa
     9061 ggagtggcag tgacatattc gattggcatt gattccggct caaccgccac caaagggatc
     9121 ttactggcag acggcgtgat tacgcgccgt ttcctcgttc caacaccctt tcgcccggca
     9181 acagcaatta ctgaagcctg ggaaactctg cgcgaagggt tagagacaac accgtttctg
     9241 acgctcaccg gctacggacg gcaactggtg gattttgccg ataaacaagt gacggaaatc
     9301 tcctgtcacg ggctgggcgc acggtttctt gagccagcaa cgcgcgcggt aatcgacatc
     9361 ggtggtcagg acagcaaagt gattcagctt gatgatgacg gtaacctgtg cgatttcctg
     9421 atgaatgaca aatgcgcggc gggcaccggg cgtttcctgg aggtgatctc gcgcacgctt
     9481 ggcaccagcg tcgagcaact cgacagcatt accgaaaatg tcataccgca cgccatcacg
     9541 agtatgtgca cagtgtttgc tgaatcagaa gtgatcagcc tgcgctctgc gggcgtagcg
     9601 ccagaagcga ttctcgccgg agtgattaac gcgatggcgc ggcggagcgc caatttcatt
     9661 gctcgtctct cctgtgaagc gccgattctg tttactggtg gcgttagcca ctgtcaaagg
     9721 tttacccaca tgctggaaag ccatctggga atgccggtac aaactcaccc tgatgcgcaa
     9781 ttcgctggtg caattggcgc ggcggtgatc ggtcagcggc agagaaaacg cgcatgaaag
     9841 agtatctgtt tttatttcac tccacgggta atgactccaa cttattgat
//
LOCUS       DAZFFE010000141         9838 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFFE010000141 DAZFFE010000000
VERSION     DAZFFE010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.141"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..634)
                     /gene="bet"
                     /locus_tag="ACHJ69_004242"
     CDS             complement(<1..634)
                     /gene="bet"
                     /locus_tag="ACHJ69_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO5017097.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIA"
     gene            complement(640..936)
                     /locus_tag="ACHJ69_004243"
     CDS             complement(640..936)
                     /locus_tag="ACHJ69_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO5017098.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAIIRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            complement(1010..1153)
                     /gene="kil"
                     /locus_tag="ACHJ69_004244"
     CDS             complement(1010..1153)
                     /gene="kil"
                     /locus_tag="ACHJ69_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO5017099.1"
                     /translation="MDKTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIH"
     gene            complement(1146..1286)
                     /locus_tag="ACHJ69_004245"
     CDS             complement(1146..1286)
                     /locus_tag="ACHJ69_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO5017100.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NG"
     gene            complement(1511..2134)
                     /locus_tag="ACHJ69_004246"
     CDS             complement(1511..2134)
                     /locus_tag="ACHJ69_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nmad5 family putative nucleotide modification
                     protein"
                     /protein_id="HFO5017101.1"
                     /translation="MSSIRLTTRMKEEIARNALIKSGVFTELEEVTKLKNQLALDARV
                     IAFGGKKKTEEVDQLASKLMAASEELQKLGCSFYSCDVSSCSIYLTVSGRRVGWHSYG
                     KDGNGEDILLPTPDKDKCMFDAEHKITKRFDEICALQQKLEARKKDIESNVWAALNSV
                     TTVKRLIEVWPESKELLPKEADKASAALPALRVEDLNKMIGLPSEAA"
     gene            complement(2196..2468)
                     /locus_tag="ACHJ69_004247"
     CDS             complement(2196..2468)
                     /locus_tag="ACHJ69_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFO5017102.1"
                     /translation="MSRKTEFKGTAASRRRARRANLQSQESISSDKLHRPTPSRVVLQ
                     CKRKPAMRAEVITLTTLTRKYEGSTCLPNVAIYAAGYRKSKQLTAR"
     gene            complement(2446..2671)
                     /locus_tag="ACHJ69_004248"
                     /pseudo
     CDS             complement(2446..2671)
                     /locus_tag="ACHJ69_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502599.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2974..3594)
                     /locus_tag="ACHJ69_004249"
     CDS             complement(2974..3594)
                     /locus_tag="ACHJ69_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017103.1"
                     /translation="MSDDSNLNNHGKQDTNISESRRLKVVGGSDFEAEFDNSPTKVQN
                     NYIKPPQTEEEVGTISREELDARLAANKAEMESIASSIRTDMALSRESVNVQFASLNA
                     AISSLSSKIDGKMDSAAGDIKAINGRFEGIQGQITGVNTAISGVQSGISIRLAIFSVI
                     IAVIVAIPGLVSALKSDHAPLQQPSTLQAPPQNTQPTDNKKITPQH"
     gene            complement(3591..4079)
                     /locus_tag="ACHJ69_004250"
     CDS             complement(3591..4079)
                     /locus_tag="ACHJ69_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017104.1"
                     /translation="MMDGRRDARRAYSDAGEFMALNLERISFITPFDSSEEPNQPTLN
                     FTCNEFPARLSIDFRVGMIGLKPNSRYNLGIMVIPAHLIIKKGEEIQFPDGSSESVSL
                     FIDTKDSHFETGVGGQVIVTLKEIRVPAKGLYSVIGILQDNEDPKNELHKNESFFTVE
                     LL"
     gene            complement(4076..4723)
                     /locus_tag="ACHJ69_004251"
     CDS             complement(4076..4723)
                     /locus_tag="ACHJ69_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO5017105.1"
                     /translation="MKTMHEIIGERIKSLREAKGLSQAQLAKLCGWAAPSRLGNYELG
                     TRKVSADDALVLGAALGVSPAKIMFGEDSDAVFRQYEYPLFSSVQAGPFSEVGSYTAS
                     DAKAWVPTTTKASEKAFWLEVKGHSMTAPQGVRPSFPEGMLILVDPAEPVESGDFCVA
                     SANGDSEATFKKYEKDAGVSYLVPLNPAYRTLDCDHTCRIIGKVVKAQWPEETFG"
     gene            4832..5050
                     /locus_tag="ACHJ69_004252"
     CDS             4832..5050
                     /locus_tag="ACHJ69_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5017106.1"
                     /translation="MNKIAQQRKKIGVSQAVLASAIGWGQSRIANYELNIRTPRLNDC
                     RKIVEGLRKLGCQCSLDDVFPPSSNKAA"
     gene            5190..5489
                     /locus_tag="ACHJ69_004253"
     CDS             5190..5489
                     /locus_tag="ACHJ69_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO5017107.1"
                     /translation="MEQTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDELTNKKRPVCKTERSEQIQMEF"
     gene            5522..6460
                     /locus_tag="ACHJ69_004254"
     CDS             5522..6460
                     /locus_tag="ACHJ69_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO5017108.1"
                     /translation="MTNTAKILNFGRSNFAGQERNVADLDDGYARLSNMLIEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAKLELVRMNIIKQQ
                     GGMFGPNKNISEWCIPQNEGGSPKMRDIPQNEGTPPKTRDKTSLKLGDCYPSKQGDTK
                     DTITKEKRKDYSSENSGESSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMV
                     KTIAPSARKPNFAGWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRD
                     KWTQLEINRNKQQAGVTAGKPKLDLTNTDWIYGVDL"
     gene            6457..7158
                     /locus_tag="ACHJ69_004255"
     CDS             6457..7158
                     /locus_tag="ACHJ69_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO5017109.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRGQNELNEIRRQWVLAFRENGITSMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            7155..7445
                     /locus_tag="ACHJ69_004256"
     CDS             7155..7445
                     /locus_tag="ACHJ69_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO5017110.1"
                     /translation="MTGKEAIIHYLGTHKNFCAQDVSAVTGATVTSINQAAAKMARAG
                     ILVIDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAVMKRVLAVYGVKR"
     gene            7531..7746
                     /locus_tag="ACHJ69_004257"
     CDS             7531..7746
                     /locus_tag="ACHJ69_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017111.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPLDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLDLK"
     gene            7757..7993
                     /locus_tag="ACHJ69_004258"
     CDS             7757..7993
                     /locus_tag="ACHJ69_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HFO5017112.1"
                     /translation="MSDLSLTQPKLKECPFCGGNARLWVEAGINIDVWGYAECDLCEA
                     RGAWAPSVAAAAEKWNRRAGDEANLSASQRSNQK"
     gene            7950..8396
                     /locus_tag="ACHJ69_004259"
     CDS             7950..8396
                     /locus_tag="ACHJ69_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO5017113.1"
                     /translation="MKQTFLLRNEAIRNNAIDAILSLPIDDKSPHEVHVKEPKRSKAQ
                     NDRMWPMLNDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSAPGIGGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSWREYEWSQRKGKAA"
     gene            8393..8920
                     /locus_tag="ACHJ69_004260"
     CDS             8393..8920
                     /locus_tag="ACHJ69_004260"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO5017114.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWVSHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            8917..9099
                     /locus_tag="ACHJ69_004261"
     CDS             8917..9099
                     /locus_tag="ACHJ69_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO5017115.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKPRPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            9378..>9838
                     /locus_tag="ACHJ69_004262"
     CDS             9378..>9838
                     /locus_tag="ACHJ69_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO5017116.1"
                     /translation="MATQISVETLSPIAYNQIPVITTELLAQLYGTETVNIKMNFSRN
                     SARFVQGKHFFKLEGNELREFKHRLSLSESVSREVTESYSVKIARNVRSLILWTERGA
                     ARHAKMLETDQAWEVFEKLEDCYFSQCEKNTGKQEKKPNGLSAKETDSLVWL"
BASE COUNT         2606 a         2287 c         2403 g         2542 t
ORIGIN      
        1 gggcgatcag cagagtgtta atctcctgca tggtttcatc gttaaccgga gtgatgtcgc
       61 gttctggctg acgttctgca gtgtatgcag tattttcgac aatgcgctcg gcttcatcct
      121 tgtcatagat accagcaaat ccgaaggcca gacgggcaca ctgaatcatg gctttatgcc
      181 gtaacatccg tttgggatgc gactgccacg gtccggtgat ttctctgcct tcgcgggttt
      241 tgaatggttc gcggcggcat tcatccatcc attcggtaac gcagatcgga tgattacggt
      301 ccttgcggta aatccggcat gtacaggatt cattgtcctg ctcaaagtcc atgccatcaa
      361 actgctggtt ttcattgatg atgcgggacc agccatcaac gcccaccacc ggaacgatgc
      421 cgttctgctt atcagggaag gcgtaaattt ctttcgtcca cggattaagg ccgtactggt
      481 tggcgacgat caacaatgcg atgaactgcg catcgctggc atcgccttta aatgccgtct
      541 ggcgaagagt ggtgatcagt tcctgtgggt cgacagaatc catgccgaca cgttcagcca
      601 gcttccctgc cagcgttgcg agtgctgtac tcatccgttt tatacctctg aatcaatatc
      661 aacctgatgg tgagcaatgg tttcaaccat gtaccggatg tgttctgcca tgcgctcctg
      721 aaactcaaca tcgtcatcaa acgcacggat aatggctttt ttgctggccc cgtggcgttg
      781 caaatgatcg atgcatagcg attcaaacag gtgctggggc aggccttttt ccatgtcgtc
      841 tgccagttct gcctctttct cttcacgggc gatctgctgg tagtgacgcg cccagctctg
      901 agcctcaaga cgatcctgaa tgtaataagc gttcatggct gaactcctga aatagctgtg
      961 aaaatatcgc ccgcgaaatg ccgggctgat taggaaaaca ggaaaggggt tagtgaatgc
     1021 ttttgcttga tctcagtttc agtattaata tccatttttt ataagcgtcg acggcttcac
     1081 gaaacatctt ttcatcacca ataaaagtgg cgatagtgaa tttagtctgg atagccataa
     1141 gtgttttatc catttttggg aactcctggc tgattaagta tgtcgataag gcgtttccat
     1201 ccgtcacgta atttacgggt gattcgttca agtaaagatt cggaagggca gccagcaaca
     1261 ggccaccctg caatggcata ttgcatggtg tgctccttat ttatttacac ataacgaaaa
     1321 cgcctctagt gaagcgttat tggtatgcat ataaaaaagc cctcacattg gagggcaaag
     1381 aagatttcca ataatcagaa caagtcggct cctgtttagt tacgagcgac attgctccgt
     1441 gtattcactc gttggaatga atacacagtg cagtgtttat tctgttgttt atgccaaaaa
     1501 taaaggccga ttatgcggcc tcggaaggaa gtccaatcat cttattcaaa tcttctaccc
     1561 gtaaagcagg aagtgctgca cttgctttat ctgcttcttt tggtagcaac tctttgcttt
     1621 caggccaaac ttcaataagt cgcttaactg ttgtgactga gttcaaagca gcccatacgt
     1681 ttgattctat atcctttttc ctggcttcaa gtttttgttg caatgcgcag atttcatcaa
     1741 acctttttgt tattttgtgt tctgcgtcaa acatgcattt atctttgtcg ggggtaggga
     1801 gcaatatatc ttcaccgttg ccgtcttttc cgtatgaatg ccagccaacc cttcttccag
     1861 atacagtcag ataaatcgaa catgaactga catcgcatga gtaaaatgaa catcccagct
     1921 tttgaagttc ctcacttgca gccattaact ttgatgccag ctggtccact tcttcggttt
     1981 tctttttccc gccaaacgca ataactctgg cgtcaagtgc aagctggttc tttaactttg
     2041 ttacttcttc aagttcagtg aacaccccgg acttaattaa agcgttacga gcgatttcct
     2101 ctttcattct cgtagttaag cggattgatg acatattaat tcctctcaaa taagtggttt
     2161 gctgcctaat ttcattttct ggcgaccaac acaagtcatc ttgctgtcag ttgtttggat
     2221 ttacggtagc ctgcagcgta aatggctacg tttggaagac aagttgaacc ttcatatttt
     2281 ctggtcaacg ttgtcagagt tataacttct gctctcattg ctggtttgcg cttgcattgc
     2341 aagaccactc gtgaaggggt tggcctgtgt agcttgtcgg agctaatcga ctcctgactt
     2401 tgcaggtttg cgcgacgagc tctacggcga gaagctgcgg tgcctttaaa ttctgttttt
     2461 ctggacatag attcctcccg aataaacttt ggcgatgcaa tctcgaagct cctcctgaga
     2521 cggttgcttc ggcattgcat cccacagctt atgtggttgg gtgatctggc ttttcagcca
     2581 cgtagtcgag tgttcgacgt tgtttaaaga gcctgccaat cttttccgtt tggctaccag
     2641 cgtcctgctg atggaaataa tagtcacata ttgtgattta atggtcaatc acaaaatgtg
     2701 taaaataagg gtgtgacacg ttatgtgtat gatttttttg tgtaaatagt tttccccgcg
     2761 atgggtttgc ttacttaagg cgggggaaac agcaggatgg tgtgctgaaa agttcgaaaa
     2821 acgagcgaag taggtggtga cgagatggcg ggatatggga gttgtattaa gaattatagt
     2881 aatttaatca gaggcttggg ctggctggca ggtgctgtcc tgatagttat gtgcaggcaa
     2941 caaaaaaccc ggcgctgagg ccgggttaat gcgttaatgc tggggagtga tttttttgtt
     3001 gtctgttggc tgggtatttt gtggcggcgc ttgcaaagtg gaaggctgtt gcaaaggtgc
     3061 gtggtctgac tttaaagctg ataccagccc gggaatcgca actattacag cgataatgac
     3121 actaaaaatg gctaatcgta tagaaatacc cgactgaaca ccactaattg cggtattaac
     3181 ccctgttatt tgtccttgaa ttccttcgaa tctcccatta attgctttta tatcgccagc
     3241 agcgctatcc atctttccat cgatcttgga cgatagagag cttatggctg cattaagtga
     3301 tgcaaattgg acattaacgc tttcacgaga tagagccatg tcagtcctaa tggaagaagc
     3361 aatagactcc atctctgctt tattagcagc taagcgagca tcaagttcct ctctgctgat
     3421 cgttcccact tcttcctccg tttgtggcgg ttttatgtaa ttattttgca ccttggtggg
     3481 agaattatca aattcagcct caaaatcgga accgccaaca accttcaacc tccggctttc
     3541 agatatgttg gtgtcttgct ttccatgatt atttaaatta ctgtcgtcac tcataatagc
     3601 tctacggtga aaaatgattc atttttatga agttcatttt tagggtcttc attatcttgc
     3661 aatatcccta taacactata cagcccttta gctgggaccc taatttcttt caatgttact
     3721 attacctgtc cgccaacccc tgtttcaaaa tggctatctt tcgtatcgat gaaaagtgaa
     3781 actgattccg aagagccgtc agggaattga atttcctcac ctttttttat aattaggtgc
     3841 gcggggatta ccataatacc caaattatat cttgaatttg gtttcaaccc gatcatacca
     3901 accctgaaat caatcgatag ccgcgcagga aattcattgc atgtaaaatt aagcgtcggt
     3961 tggttgggtt cttcgctgct atcaaatggg gttataaaag atattctttc taaattaagt
     4021 gccataaatt ctcctgcatc gctgtatgct ctacgagcat ctctgcgacc atccatcatc
     4081 caaacgtctc ttcaggccac tgagccttaa ctaccttgcc tatgatgcgg catgtgtgat
     4141 cgcagtccag ggttctgtat gccggattca atggaaccaa gtaactaacc cctgcatcct
     4201 tctcatactt cttgaacgtt gcctctgaat caccatttgc agaagccacg cagaaatccc
     4261 cagactctac cggctcggcc ggatcaacga gtatcagcat accctcagga aaactcggcc
     4321 ttacgccctg tggcgcagtc atagaatggc ctttcacctc aagccagaaa gctttttcgc
     4381 tggcttttgt ggtcgttggg acccatgcct ttgcatcgct tgctgtgtag cttccaacct
     4441 ccgaaaacgg cccggcctgc actgaagaaa ataacgggta ctcatattgg cgaaatacag
     4501 cgtctgaatc ctcgccaaac attatttttg ccggagatac gccgagtgcc gccccaagaa
     4561 ccagcgcgtc atccgcgcta acctttcttg ttcctaactc gtagttcccc aggcgtgaag
     4621 gcgcagccca gccgcaaagc ttggccaatt gagcctggct aagtccttta gcttctctaa
     4681 gggacttgat cctttccccg ataatttcat gcatcgtttt catcctttaa atgtaacacg
     4741 caacgtgatt gaactctgta cacgaattga ggttgactgt taatcacaaa ttgtgtgtaa
     4801 tgggtgtgtg attaatacta gggagaccgc aatgaacaaa attgcccagc agcgaaaaaa
     4861 aatcggagtt tcgcaagctg tactggcttc ggcaattggt tgggggcaat cccgcatcgc
     4921 caactatgag ctgaatatcc gtactcctag acttaacgat tgccgaaaga tcgtagaagg
     4981 cctcaggaag ttagggtgcc aatgttcttt ggatgatgtt ttccctccat ccagtaacaa
     5041 agccgcctaa gcagtatccg ctctttgtaa caacggacat tcgtcctacg tcgctgaaaa
     5101 gcgagtccca agatatctga ccaactaagg ccatatgcgt ttccacgcat acctttcaac
     5161 taactattca ctattggaaa tcttaagaaa tggaacaaac aagttacagc aaactatcac
     5221 agcgcgacgt tgatcgcgca gaaacagatt tacttatcaa cctgtcaacg cttacccagc
     5281 gcggtctggc aaagatgatt ggctgtcatg aatcgaagat aagcagaacg gactggagat
     5341 ttattgcttc ggtcttgtgt gctttcggaa tggcatcaga catcagtccg attagcaggg
     5401 cttttaagta tgcgcttgat gaactcacca ataaaaaacg cccggtgtgc aagaccgagc
     5461 gttctgaaca aatccagatg gaattctgag gtcattactg gatctatcaa caggagtcat
     5521 tatgacaaat acagcaaaaa tactcaactt cggcagaagt aactttgccg gacaggagcg
     5581 taatgtggca gatctcgatg atggttacgc cagactatca aatatgctga ttgaggctta
     5641 ttcaggcgca gatctgacca agcgacagtt taaagtgctg cttgccattc tgcgtaaaac
     5701 ctatgggtgg aataaaccaa tggacagaat caccgattct caacttagcg agattacaaa
     5761 gttacctgtc aaacggtgca atgaagccaa gttagaactc gtcagaatga atattatcaa
     5821 gcagcaaggc ggcatgtttg gaccaaataa aaacatctca gaatggtgca tccctcaaaa
     5881 cgagggaggt tcccctaaaa tgagggacat ccctcaaaac gagggaaccc cccctaaaac
     5941 gagggataaa acatccctca aattagggga ttgctatccc tcaaaacagg gggacacaaa
     6001 agacactatt acaaaagaaa aaagaaaaga ttattcgtcc gagaattctg gcgaatcctc
     6061 tgaccagcca gaaaacgatc tttctgtggt taaaccggat gctgcaattc agagcggcag
     6121 caagtgggga acagcagaag acctgaccgc cgcagagtgg atgtttgaca tggtgaagac
     6181 catcgcacca tcagccagaa aaccgaattt tgcagggtgg gctaacgata tccgcctgat
     6241 gcgtgaacgt gacggacgta accaccgcga catgtgcgtg ctgttccgct gggcatgcca
     6301 ggacaacttc tggtccggta acgtgctaag tccggccaaa ctccgcgaca agtggaccca
     6361 actcgaaatc aaccgtaaca agcaacaggc tggcgtgaca gctggaaaac caaaactcga
     6421 cctgacaaac actgactgga tttacggggt ggatttatga aaaacatcgc cgcacagatg
     6481 gttaactttg accgtgagca gatgcgtcgg atcgccaaca acatgccgga acagtacgac
     6541 gaaaagccgc aggtacaaca ggtagcgcag atcatcaacg gtgtgttcag ccagttactg
     6601 gcaactttcc cggcgagtct ggctaaccgg ggccagaacg aactgaatga aatccgccgc
     6661 cagtgggttc tggctttccg ggaaaacggg atcacctcga tggaacaggt taacgcagga
     6721 atgcgcgtag cccgtcggca gaatcgacca tttcttccat cacccgggca gtttgttgca
     6781 tggtgccggg aagaagcatc cgttatcgcc ggactgccaa acgtcagcga gctggttgat
     6841 atggtttacg agtattgccg gaagcgtggc ctgtatccgg atgcagagtc ttatccgtgg
     6901 aaatcaaacg cgcactactg gctggttacc aacctgtacc agaacatgcg ggccaatgcg
     6961 ctgactgacg cggaattacg gcgcaaggct gccgatgaac tgacctgtat gacagcacga
     7021 attaaccgtg gtgagacgat acctgaacca gtaaaacaac ttcctgtcat gggcggcaga
     7081 cctctaaatc gagcacaggc tctggcgaag atcgcagaaa ttaaagctaa gttcggactg
     7141 aaaggagcaa gtgtatgacg ggcaaagagg caattattca ttacctcggg acgcataaga
     7201 acttctgtgc acaggacgtt tccgcggtaa caggcgcaac cgtaaccagt ataaatcagg
     7261 ctgcggctaa aatggcgcgg gcaggaatcc ttgtcattga tggtaaggtc tggcgaacgg
     7321 tgtattaccg gtttgctacc agggaagaac gggaaggaaa ggtgagcacg aacctgattt
     7381 ttaaggagtg tcgccagagt gcagtgatga aacgggtatt ggcggtatat ggagttaaaa
     7441 gatgaccatc tacatcactg agcttgtaac aggcctgctg gtaatcgcag gcctttttat
     7501 ttggggaaga gtaattggag gctttaagaa atgagtacga tagctgagct tgtcagggct
     7561 aattttcgtg aagagttggt gcgttggtat cggtatcgtt catcgtccag tttgccgctt
     7621 gatgagttgt atgagcattc acctgccgca cgacgctatc cgcgtgaccg tgttcttcga
     7681 cggttgttca aactcaacaa tgagtttcag cgcaacagaa ttatccggag tctggattta
     7741 aagtgaagga gtgagcatga gcgacctatc attaacccag ccaaagctaa aagaatgtcc
     7801 gttttgcggc ggtaatgctc gtctgtgggt tgaggccgga ataaatattg atgtgtgggg
     7861 ctatgcagaa tgtgacctct gtgaagccag gggggcatgg gcaccatcag ttgctgcggc
     7921 ggctgaaaaa tggaaccgga gagcaggaga tgaagcaaac ctttctgctt cgcaacgaag
     7981 caatcagaaa taacgccata gacgccattc tctcactacc catcgacgac aagtcacccc
     8041 acgaagtcca cgttaaagaa cccaagcgta gcaaagcgca gaatgaccgt atgtggccga
     8101 tgctgaacga tgtttcgcgt caggtgctat ggcatggtca acggctggcg ccggaagact
     8161 ggaaagacct gttcactgcc ctgtggctta agaccaaaaa actggagcaa cgaagtgcgc
     8221 ctggtatcgg cggtggcgtt gtcatgcttg gtgtgcgcac cagcaaaatg cggaaggcca
     8281 gcatgactga gcttatcgaa atcatgttct ggttcggctc agagcgcaac gtgcggtgga
     8341 gtgatgactc ctggcgagag tatgaatggt cacaacgaaa agggaaggct gcatgactat
     8401 caaatcaaat acgccagcac acgacaagga ctgctggcaa acgccgcttt ggctttttga
     8461 tgcactggat attgagtttg gattctggct ggattcggca gcgagcgaca aaaatgctct
     8521 gtgtgctcac tggctaactg aggccgacga cgcgctcaat tctgagtggg taagccacgg
     8581 tgcaatctgg aataacccac cgtacagcaa tatcaggccg tgggtggaaa aagccgctga
     8641 gcagtgcata caacaacgac agacggtagt gatgcttgtg ccagaggata tgtctgtcgg
     8701 atggttcagt aaggctctgg agagtgttga cgaagttcgc atcatcactg atggacggat
     8761 taattttatc gaaccatcga cagggctgga gaagaaggga aacagcaaag gttccatgct
     8821 gctgatttgg cgaccgttca tcagtcctcg acggatgttt actaccgtat ccaaagcggc
     8881 attgatggcg atcgggcagg gcgtcaggag ggcggcatga ggcgacagcg acgaagtatc
     8941 accgacatca tctgcgaaaa ctgcaaatac cttccaacga aacgctccag aaataaaccc
     9001 aggccaatcc caaaagaatc tgacgttaaa accttcaatt acacggctca cctgtgggat
     9061 atccggtggc taagacatcg tgcgaggaaa acaaggtgat tgacgtgatg atttattcgg
     9121 ggctatattc ctcacgcgcc agcaaaatct ggcgtcggga tttgcacccc ggatattcaa
     9181 ccgcgacaga cacacgccgc gagcgtgttt tttgttgtcg taagcacacg cacatctgaa
     9241 ttatggtggg gcgtatgggg gagccgaaag gctcgccggt tggttgatcc ggtagtgcaa
     9301 accctgtacg tctcaccacc caaagatttg cacctgacgg tggtgatagt taaattcatc
     9361 aaccagaggg cgttgtcatg gcgactcaaa tttccgttga aactctttcc ccaattgcct
     9421 acaaccagat ccccgtaatt actacagaat tattggcgca attgtatggc actgaaacag
     9481 tcaatataaa aatgaatttt tcccgtaact ctgcgcgatt tgtacaggga aaacatttct
     9541 ttaagttgga agggaatgaa ctacgtgagt ttaagcacag actatcttta agcgagtctg
     9601 ttagccgcga ggtaacagaa agttactctg tgaaaatcgc ccgcaatgtt cgctccctca
     9661 tcctctggac agaacgcggc gcagcccgtc acgcaaaaat gctcgaaacc gatcaggcgt
     9721 gggaagtgtt cgaaaaactg gaagactgct atttcagcca gtgcgagaaa aatactggca
     9781 aacaagagaa gaagcccaac gggctttccg caaaagaaac agacagcctt gtatggct
//
LOCUS       DAZFFE010000142         9799 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFFE010000142 DAZFFE010000000
VERSION     DAZFFE010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.142"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..780)
                     /locus_tag="ACHJ69_004263"
     CDS             complement(<1..780)
                     /locus_tag="ACHJ69_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO5017117.1"
                     /translation="MKNIKLLGSAMLVSLALFSQSSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLHGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQ"
     gene            complement(816..1670)
                     /locus_tag="ACHJ69_004264"
     CDS             complement(816..1670)
                     /locus_tag="ACHJ69_004264"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HFO5017118.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLRVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
     gene            2012..2842
                     /locus_tag="ACHJ69_004265"
     CDS             2012..2842
                     /locus_tag="ACHJ69_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5017119.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKQLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            2874..3809
                     /locus_tag="ACHJ69_004266"
     CDS             2874..3809
                     /locus_tag="ACHJ69_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO5017120.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFTRLIIERLRHEGVITNGIHVMNNAVTGTAFVSYQNPQLRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVAGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQY"
     gene            3902..4144
                     /gene="yhdT"
                     /locus_tag="ACHJ69_004267"
     CDS             3902..4144
                     /gene="yhdT"
                     /locus_tag="ACHJ69_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO5017121.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            4134..5585
                     /gene="panF"
                     /locus_tag="ACHJ69_004268"
     CDS             4134..5585
                     /gene="panF"
                     /locus_tag="ACHJ69_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO5017122.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVDTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            5597..6478
                     /gene="prmA"
                     /locus_tag="ACHJ69_004269"
     CDS             5597..6478
                     /gene="prmA"
                     /locus_tag="ACHJ69_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO5017123.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            6807..7772
                     /gene="dusB"
                     /locus_tag="ACHJ69_004270"
     CDS             6807..7772
                     /gene="dusB"
                     /locus_tag="ACHJ69_004270"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO5017124.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            7798..8094
                     /gene="fis"
                     /locus_tag="ACHJ69_004271"
     CDS             7798..8094
                     /gene="fis"
                     /locus_tag="ACHJ69_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO5017125.1"
                     /translation="MFEQRVNSDVLTVSIVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            8180..9064
                     /gene="yhdJ"
                     /locus_tag="ACHJ69_004272"
     CDS             8180..9064
                     /gene="yhdJ"
                     /locus_tag="ACHJ69_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO5017126.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQYRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     NYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            9148..9327
                     /gene="yhdU"
                     /locus_tag="ACHJ69_004273"
     CDS             9148..9327
                     /gene="yhdU"
                     /locus_tag="ACHJ69_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO5017127.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFHDRM"
     gene            complement(9330..>9799)
                     /gene="acrS"
                     /locus_tag="ACHJ69_004274"
     CDS             complement(9330..>9799)
                     /gene="acrS"
                     /locus_tag="ACHJ69_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO5017128.1"
                     /translation="QPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAKIPRQQAL
                     LKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCIANNLDLDVVMIIID
                     GAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELSVI"
BASE COUNT         2483 a         2375 c         2366 g         2575 t
ORIGIN      
        1 ttgccgaatc acggccgcaa acgttttttc actgtcaacg gcatcacgcg cttctttcat
       61 gccgtcaaaa ccaatgattt tgatgttgtc gttatgcgca gattttgctg cgataaccgc
      121 tgccagcgcc gcatcatcac caaagccaaa cagaccattc aggttggcgt ttgcctgcat
      181 aatgttttgc gcagtcgtta acgcttcagc ccgcgtaata ccaggctgtt cagcaacaat
      241 tttaatatcc ggtgtatcgc ttaacccttt cttaaaacca tcaacgcgcg caaccaccga
      301 ttgtagtgcc ggataagaaa taatggctac agtaccttta ccatgcaaaa gtttacccat
      361 taacttgcct gcttcaacgc ctcccgcata gttatcggtc gcaacatgcg atacgaccgg
      421 aacgccttcc gcagcgacgt caacggtgat aacgggaata ccggccttat cggctttaat
      481 tatcgctgcc tggacgcctt tcgaatcgac cggagagata ataatggcat ccactttttt
      541 agtaataaaa tcctcaacat cagaaagttg tttgtttaaa tcctgattgg caatagagac
      601 acttaaattg acattgtctt tttttgcctc ttctttcatg gcattcgcaa gatcaatata
      661 gaaaggatgt tgttgtgtta ataatgacgc accaatttta tactcttttg ccaacgaact
      721 ttgcgagaac aatgccaggc tcaccaacat agctgaacca agtaatttaa tatttttcat
      781 aaacaatcct cattattttt gtagggtaca acgtcttaaa tttttccttc actaccacag
      841 acgccaataa tacgagttac gacttctttc atggcggcct ttgcgggctg catatagtga
      901 cgtggatcat ttgccgcagg attgttgtag aaatatcctt ttaacgcatc ggcaaacgca
      961 attttcaatt ctgtcgcgac attcacttta caaataccgc gacgaataca gtgctgaacg
     1021 tcctgttccg ataagcccga agcaccatgc aacaccagcg gaatctccac tcgctgacga
     1081 atctgttcca gccgcacaaa atccagtcgt ggttcatgcg catataaacc atgtgctgtg
     1141 ccgattgcta ccgccaggga gtcaatttgg gtacgttgaa caaactctgc agctgcggct
     1201 ggtgaggtat aagcggaatc tttttcatcc acccgcagat cgtcctcctg gccgccaaga
     1261 cgccctagct cggcctcaac gctaacatca aagcgatggc aaaaggtggt aacttcttgc
     1321 actcgcgcaa tgttgtcttc aaatggcaaa tgggagccat caatcatgac agaacggatc
     1381 cctgcgcgaa ccttgttaaa aatgtcatca ctctcttcat gatggtcgag atgcaggact
     1441 aacggcaggt tgtactcgcg agccagcgat tgtactaacg aaattaaact tcccacgcca
     1501 ccataacgat atgtaccggg tgttcccgcc agcataacgg gagagcgaag ttgtgcagcg
     1561 ctctcaacaa ctacctgtaa agtttcaagg ttatgaatgt tgaaagcagg gacggcatat
     1621 ccctctgact gggctttttt cagcatgttt ttgcttgaaa taatatacat cgcagctcct
     1681 gttatttgtc gttcgcctta caaaatgaag aaagagaatc tttactttca ttttgaaagc
     1741 aaactagcac agcaaaagca gcaaaaagcg tgatgcatgt cgaacatcat caaaataact
     1801 tacaaaaaat aacttaattt atttttcact ttgcaagtga aaccaatgga aaccctgcat
     1861 aaatatgaat atattcattt atttttcaat gtattaagct attttcgttg caatttttga
     1921 cccctgattt tcttttcgaa ttgtcgatat gattgaagga taactttcaa agaaaaatac
     1981 aaggggctat agtgttacgc attaaaggcc aatgaagagt gttaacgtgc cgagaaaatc
     2041 gtctaaccta ctgaaacgca gattaaaaat tgcagaaatt gttgccgccc agggtgaaat
     2101 caaagttgat gacttatctg cgcaacttgg tgtctctggc gtgacgatcc gcggcgatct
     2161 cagttatctg gagcaacagg gatatttaaa gcgatcattt ggcggtgcta ttgcgacacc
     2221 actctcctct gaaccagaag cgatacaacc gatcgcgcca ttgtctctgg cacaacaaat
     2281 ggaaatagca cgacattgcg cacggttaat tcgcgatcgt gacacgctat ttcttggaca
     2341 tgggacaatc tgccgtaaga tcattccatt gctcagcgaa gtgaaaaaac tgcgtctgat
     2401 caccaacgat cccgaacatg cgctgctggc aaatcagttt attgatggcg aaataatact
     2461 ggctggcagt gaaatgttgc gccccgacac cgcactggcc ggaaaacagc ttgaacaggc
     2521 acttcagcat ttcaccatta cacatagcat tctggaaatc agccatctgg atgctgatgg
     2581 tactttaaat atcaacgttc ccagactcgc cgctgcctgg cagctctgtt tcgatcatgc
     2641 ccagaataag acggttatcg ttgccgccga tcctgcgcca tcaacggctg ctatcgggca
     2701 tttgcatcag gcagaaaatg tgatcgtcag ccgcagcatt aacgaacaat atcagcaaca
     2761 attgcagtcg gctgatttcg ttatccttta taccagtaat gagtgcttta cctggtccaa
     2821 tcgcgaaagg cttcaggaat gagaggggtt ttgtgacaat aactatctgc gctatgggcg
     2881 aattgctggc cgagtttttg tcccgcaacc cacatcaaaa attcactcag cctggggagt
     2941 ttatcgggcc atttcccagc ggtgcgccag caatttttgc tgctcaggtg gcaaaactgt
     3001 cccatcgggc catcttcttt ggatgtgttg gtaatgatga ttttacccga ctcattatag
     3061 agcgtctccg tcatgaaggt gtcattacca atgggatcca tgttatgaac aatgccgtca
     3121 caggtacggc gttcgtgagt tatcaaaatc cccagctgcg ggatttcgtc tttaatatcc
     3181 ctaacagcgc ctgcggtttg tttactgccg agcacattga taaggatctg cttaaacagt
     3241 gtaaccatct gcatattgtg ggctcatcgt tgttctcatt tcgcatgatc gatgtcatgc
     3301 gtaaagcaat aacgacgatc aaatcggctg gcggcaccgt ttctttcgat cccaatattc
     3361 gcaaagagat gctgagcatt cctgaaatgg cgcaggctct cgattatttg attgaatata
     3421 cggatatttt tatccccagc gaaagcgaac tccctttctt cgcgcgtcac aaaaatctgt
     3481 cagaggaaca gattgttagc gatcttctcc acggcggcgt aaaacatgtg gcgataaaac
     3541 gcgcccagcg tggggccagc tattacaagc ttaaaaacgg tacattacac gcccagcatg
     3601 ttgcaggtca cgatatcgaa attatcgatc caacgggtgc aggcgactgc tttggcgcaa
     3661 cgtttatcac tcttttctta tccggtttcc cggcacacaa ggcgctgcaa tatgcaaatg
     3721 ccagcggcgc gctcgccgta atgcggcaag gtccgatgga agggatatcc tcactggcag
     3781 acattgaaga ctttttgcag cagtactgaa cggcaatttt ctgtcagatc attttccctg
     3841 cctgaagact tgccgtagaa ttgcccgttt ctcgtatccc cccatgagga ggaccccttc
     3901 cgtggacact cgttttgttc aggcccataa agaggcgcgc tgggcgctgg ggctgaccct
     3961 tttgtatctg gcggtatggt tagtagccgc ttacttacct ggcgttgccc ccggttttac
     4021 cggctttccg cgctggtttg agatggcctg catcctgacg ccgctgctgt ttattggact
     4081 gtgctgggcg atggtgaaat ttatctatcg cgatatccca ctggaggatg acgatgcagc
     4141 ttgaagtaat tctaccgctg gtcgcctatc tggtggtggt gttcggtatc tctgtttatg
     4201 cgatgcgtaa acggagcact ggcaccttcc ttaatgagta tttcctcggc agccgctcta
     4261 tgggcggtat tgtgctggcg atgacgctca ccgcgaccta tatcagtgcc agttcgttta
     4321 tcggcgggcc tggagctgct tataaatacg gcctgggctg ggtattgcta gcgatgattc
     4381 agcttcctgc agtctggctt tcgctcggta ttctcggcaa gaagttcgca attcttgcgc
     4441 gccgctacaa tgcggtgacg ctgaacgata tgctgtttgc ccgctaccag agtcgtcttc
     4501 tggtatggct ggcgagtttg agtttgctgg ttgcgttcgt tggtgcgatg accgtgcagt
     4561 ttatcggcgg tgcgcgcctg ctggaaaccg cggcgggtat tccttatgaa accgggctgc
     4621 tgattttcgg tatcagcatt gcgttgtata ccgcctttgg cggctttcgc gccagcgtgc
     4681 tgaacgacac catgcaaggg ctggtgatgc tgattggcac cgttgtgctg cttattggcg
     4741 tggtacatgc cgcaggtggt ttaagtaatg cggtagacac gctgcaaacc atcgatccgc
     4801 aactggttac gccacaaggc gctgacgata ttctgtcgcc tgcctttatg acgtcgttct
     4861 gggtactggt gtgctttggc gttataggcc tgccgcatac tgcggtgcgc tgtatctctt
     4921 ataaagacag caaagccgta catcggggaa tcattatcgg tacgattgta gtcgcaatcc
     4981 tgatgttcgg tatgcatctg gccggagcgt taggtcgggc ggtgatcccc gatctcaccg
     5041 taccggatct ggtgatccca acgttaatgg taaaagtgct gccaccgttt gctgccggga
     5101 tcttcctggc tgcgcctatg gctgcgatca tgtcgacaat taacgcccaa ctgctgcaaa
     5161 gttccgctac gatcattaaa gatctctatc tgaatatccg tccggatcaa atgcaaaacg
     5221 agacgcgtct gaagcggatg tcggcggtga tcacgttggt tctcggtgcg ttgctgctgc
     5281 ttgccgcctg gaagccgcca gaaatgatca tctggctgaa tttgctggcc ttcggtgggc
     5341 tggaagccgt tttcctgtgg ccgctggtgc tgggtcttta ctgggaacgc gccaacgcca
     5401 aaggcgcgct aagtgcgatg atcgttggcg gcgtgctgta tgccgtactc gcgacgctga
     5461 atattcagta cctgggcttc caccctatcg tgccctcgtt actactaagt ttgctggctt
     5521 tcctggtcgg aaaccgtttc ggtacatccg tcccgcaagc taccgttttg actactgata
     5581 aataaagagt tttgccatgc cttggatcca actgaaactg aacactaccg gcgcgaacgc
     5641 ggaagatctt agcgatgcgc tgatggaagc gggtgccgtt tctatcactt ttcaggatac
     5701 ccacgatacg ccagtatttg agccgctgcc gggcgaaacg cgcctgtggg gcgacaccga
     5761 tgtgattggt ctgttcgacg ctgaaaccga tatgaacgac gtggtggcga ttctggaaaa
     5821 ccatccgctg ctcggcgcgg gcttcgcgca taaaatcgaa caactggaag ataaagactg
     5881 ggagcgcgaa tggatggata atttccaccc gatgcgcttt ggtgaacggc tgtggatctg
     5941 cccaagctgg cgtgatgtgc cggacgaaaa cgccgtcaac gtgatgttag acccagggct
     6001 ggcgtttggt acgggtaccc atccaactac ctctctgtgc ctgcaatggc tcgacagcct
     6061 cgatttaacc ggtaaaacgg tcatcgactt tggctgtggt tccggcattc tggctatcgc
     6121 ggcgctgaag ctgggtgcag caaaagccat tggtattgat atcgacccgc aggcgattca
     6181 ggccagccgc gacaacgccg aacgtaatgg cgtttctgac cgtctggaac tctacctacc
     6241 taaagatcag ccagaagaaa tgaaagccga cgtggtggtc gctaacatcc ttgcaggccc
     6301 attacgtgaa ctggcaccgt taatcagcgt cctgccggtt tcaggtggct tgctgggcct
     6361 ttccggtatt ctggcaagcc aggcagagag cgtttgtgaa gcttatgccg acagcttcgc
     6421 tctggacccg gtcgtggaaa aagaagagtg gtgccgcatt accggtcgta agaattaacc
     6481 ttcgcatcgc cgtagggtga cgcaaggcga agtgcgagca aggtcacaaa aggcacgtta
     6541 atttgccgat tatttacgca aatttgcgtg ccaaaatttt cattcataaa gaaaaattga
     6601 gaacttactc aaatttcttg gagtgtaaat tttagtcact attttctaat ataatgattt
     6661 ttatgagtaa ttatcgcgcc aagctcattt taaatgcaat tctttgatcc atctcagatg
     6721 attggtcaaa gtttggcctt tcatctagtg caaaaaatgc gtaatatacg ccgccttgca
     6781 gtcacagtat ggtcatttct taactcatgc gcatcggaca atatcagctc agaaatcgcc
     6841 tgatcgcagc gcccatggct ggcattacag acagaccttt tcggacgttg tgctacgaga
     6901 tgggagccgg attgacagta tccgagatga tgtcttctaa cccacaggtt tgggaaagcg
     6961 acaaatctcg tttacggatg gtgcacattg atgaacccgg tattcgcacc gtgcaaattg
     7021 ctggtagcga tccgaaagaa atggcagatg cagcacgtat taacgtggaa agcggtgccc
     7081 agattattga tatcaatatg ggttgcccgg ctaaaaaagt gaatcgcaag ctcgcaggtt
     7141 cagccctctt gcagtacccg gatgtcgtta aatcgatcct taccgaggtc gtcaatgcag
     7201 tggacgttcc tgttaccctg aaaattcgca ctggctgggc accggaacac cgtaactgcg
     7261 aagagattgc ccaactggct gaagactgtg gcattcaggc tctgaccatt catggccgta
     7321 cacgcgcctg tttgttcaat ggagaagctg agtacgacag tattcgggca gttaagcaga
     7381 aagtttccat tccggttatc gcgaatggtg acattactga cccgcttaaa gccagagctg
     7441 tgctcgacta tacaggggcg gatgccctga tgataggccg cgcagctcag ggaagaccct
     7501 ggatctttcg ggaaatccag cattatctgg acactgggga gttgctgccc ccgctgcctt
     7561 tggcagaggt taagcgcttg ctttgcgcgc acgttcggga actgcatgac ttttatggtc
     7621 cggcaaaagg gtaccgaatt gcacgtaaac acgtttcctg gtatctccag gaacacgctc
     7681 caaatgacca gtttcggcgc acattcaacg ccattgagga tgccagcgaa cagctggagg
     7741 cgttggaggc atacttcgaa aattttgcgt aaacagaaat aaagagctga cagaactatg
     7801 ttcgaacaac gcgtaaattc tgacgtactg accgtttcta tcgttaactc tcaggatcag
     7861 gtaacccaaa aacccctgcg tgactcggtt aaacaggcac tgaagaacta ttttgctcaa
     7921 ctgaatggtc aggatgtgaa tgacctctat gagctggtac tggctgaagt agaacagccc
     7981 ctgttggaca tggtgatgca atacacccgt ggtaaccaga cccgtgctgc tctgatgatg
     8041 ggcatcaacc gtggtacgct gcgtaaaaaa ttgaaaaaat acggcatgaa ctaattcagg
     8101 ttagctaaat gcttgattaa aaaggcgcta ctcggcatgg ggaagcgcct tttttatagg
     8161 tgtcacaaag ggagtgacca tgagaacagg atgtgaaccg acccgatttg gtaatgaagc
     8221 aaagaccatc atccagggtg atgcattaac cgaacttaaa aagctccccg ccgaaagtgt
     8281 cgatctgatc tttgccgacc caccgtataa catcggtaaa aattttgatg gtctgatcga
     8341 agcctggaaa gaagatctgt ttatcgactg gctgtttgaa gtgattgcag agtgccatcg
     8401 cgttctgaaa aagcagggca gcatgtacat catgaatagc acggaaaaca tgccctttat
     8461 cgatctccag taccgcaagc tttttaccat caaaagtcgc atcgtctggt catatgacag
     8521 ttctggagtg caggcgaaaa aacactacgg ctccatgtac gaacccatcc tgatgatggt
     8581 gaaagacgca aagaactaca cgttcaacgg tgatgctatt ctggtagaag ccaaaaccgg
     8641 atcgcagcgc gcgttgatca attatcgcaa aaatcctcca cagccttaca atcatcaaaa
     8701 agtaccgggt aacgtctggg attttccgcg cgtgcgttat ttgatggatg aatatgaaaa
     8761 ccacccgacg caaaaaccgg aagccttgct gaaacgcatt attctcgcct cttccaaccc
     8821 aggcgatatc gttctcgacc cgtttgccgg tagctttact accggtgccg tagccgtcgc
     8881 cagcgggcga aaattcattg gtattgagat caacagcgag tacatcaaaa tggggcttcg
     8941 acggctggat gtcgcgtcgc attactctgc ggaagaactg gcgaaagtga aaaaaagaaa
     9001 gaccggcaac ctgtcaaaac gaagccggtt aagcgaagtt gaccccgatc tcattgcaaa
     9061 gtaaaggaag tgtaagcctg gttttcagat tattcatttc gtgtatattt cctgccagac
     9121 ttggttaaac atgcacaggc tcaggtaatg attcgcaagt attggtggct cgtcgttttc
     9181 gctgtcttcg tttttctgtt tgatacttta ctgatgcaat ggattgaact gctggcaaca
     9241 gaaacagaca aatgccgcaa tatgaactca gttaatccac taaaactggt taactgtgac
     9301 gaactgaatt ttcacgacag aatgtgaatt tatatgacac ttaattcatt cgtttgatga
     9361 attaatttcg ttatgttttc atctggcatg aacattctta atacgttatc gaccagagcg
     9421 ggggcttgtt tataaagatc ataacccgcc atattcatta accagttttg aacaattccg
     9481 ctgaaggcac catcaataat aatcatcaca acatctaaat cgagattatt tgctatacaa
     9541 ccttgttgct gacacgcctg caatacttcg cggagagtct gcggattaaa gcccatcttt
     9601 tcgcgtatca ctccctcggc cagcatctca tcattaaatt cacatttgtg atataagatt
     9661 ttcagcaacg cctgctggcg gggaattttg gcaatatatt gcaaaccgac aatcaatttt
     9721 tcacgcaatt gctgaaacgg gtcatgctct aacccagcgg tcaagtgatc ctggattaac
     9781 tcccgcaatg aaggctgtt
//
LOCUS       DAZFFE010000143         9626 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFFE010000143 DAZFFE010000000
VERSION     DAZFFE010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.143"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1930
                     /locus_tag="ACHJ69_004275"
     CDS             <1..1930
                     /locus_tag="ACHJ69_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFO5017129.1"
                     /translation="DDAGFDEEDFKKPIRVHLPVVNDLPPEGVFDTEFCNRYEKGGND
                     GITMMAIPFDDNINGEDATTAGDDNDNLDGTIPDDVEKSESPDSDDDCSECEIPVATL
                     SLTHRFLHLFLFSKDEDGKYRHHATPEQRNNVIRMEMDTEDSYLQSLLTAVRAAHHEL
                     DKLTNYHLSRLAESVGKAFPHSANHRISPAEFDKFISTWMKTDYLDQGLLTKEWQNGN
                     YVSGITRTPSGANAGGGNITDRGEGFKHDKTSLARDVATGVLARSMDVDIYNLHPAHA
                     KRVEEIVSENKPPFSVFRDKFIAMPGGLDYSRAIVVASVKEAPIGIEAIPARVTEYLN
                     KVLTETDHANPDPEIVEIACGRSSAPMPQRGTAEGKHGDEEKQQASDTMANEQAAPES
                     VEEIPVKHNEDTQSLENVSSVETKYQELREELNKARENIPPKNPVDADKLLAASRGEF
                     VEGISNPADPKWVKGIQTRDTEDQNQSKVEQIAPEAGQNSPDTQQNGPEEQQPGPVAQ
                     PELEKNCRVCGQTGGGNCPDCSAVMGDSTYTETFGENDAADGEDSAQTEEKIIQENSV
                     DAAQEGETVVQNEPGSDTSGDDANSEPVTLDWKRQLVIAAVYGLCANPACIATAPAIP
                     DIAIMIANRLENFGGDKS"
     gene            1927..2223
                     /locus_tag="ACHJ69_004276"
     CDS             1927..2223
                     /locus_tag="ACHJ69_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO5017130.1"
                     /translation="MNAWLIPDRIEEQSWARHYQQIAREETEAELADDLEKGLPQHLF
                     ESLCIDNLQRHGASKKAISRAFDDDVDFQERMAEHIRYMAETIARHQINIDSEV"
     gene            2229..3014
                     /gene="bet"
                     /locus_tag="ACHJ69_004277"
     CDS             2229..3014
                     /gene="bet"
                     /locus_tag="ACHJ69_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO5017131.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRRAPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTTERQPERDITPVNEETMSEINALLTSMEKTWDD
                     DLLPLCSQIFRRNIYTSSELTQAEAVKVLGFLKQKVTEQKVAA"
     gene            3011..3691
                     /locus_tag="ACHJ69_004278"
     CDS             3011..3691
                     /locus_tag="ACHJ69_004278"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO5017132.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARALFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMEVFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            3739..3990
                     /locus_tag="ACHJ69_004279"
     CDS             3739..3990
                     /locus_tag="ACHJ69_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5017133.1"
                     /translation="MFVLIQRGQSFVDANNYPVQVCKVTLTQVIFRRLDGKTRAASIN
                     SFNAEFERIDHNELHMIKAEIEKEKHIASLRKMRRTSIN"
     gene            4036..4320
                     /locus_tag="ACHJ69_004280"
     CDS             4036..4320
                     /locus_tag="ACHJ69_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO5017134.1"
                     /translation="MREFVNQHSHGIQPVITPVVQINANEWVTLELLMAVTGLRKGTI
                     LRARDAAWMNGREYKQIAPDGTPKKNSECLYHLPTINTWIKNQPLPSQDV"
     gene            4373..5683
                     /locus_tag="ACHJ69_004281"
     CDS             4373..5683
                     /locus_tag="ACHJ69_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5017135.1"
                     /translation="MANSAYPAGVENHGGKLRITFKYRGKRVRENLRVPDTPKNRKIA
                     GELRASVCFAIRTGTFDYAERFPDSPNLKLFGLVKKDITVGELAQKWLTLKAMEISSN
                     ALNRYQSVMKNMLPRLGVGRLASSITKEDLLFIRKDLLTGEKESRKNTSTNKGRTVPT
                     VNYYMTTTAGMFSFAAENGYLEKNPFNSITPLRKSKPVPDPLTREEFGRLIDACHHQQ
                     TKNLWTVAVFTGMRHGEIAALAWEDIDLKVGTITVRRNFTKIGDFTLPKTDAGTDRVI
                     HLLAPAIEALKNQAMLTRLGRQHQITVKLREYGRTILHECTFVFCPQIVRKNHKAGIN
                     YAVSSIGATWDSAMKRAGIRARKAYQSRHTYACWALSAGANPTFIASQMGHSSASMVY
                     NVYGAWMPECSVTQVAMLNNVLNARAPDVPQSDQEDEIKLYFSK"
     gene            5680..6258
                     /gene="wrbA"
                     /locus_tag="ACHJ69_004282"
     CDS             5680..6258
                     /gene="wrbA"
                     /locus_tag="ACHJ69_004282"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000607019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO5017136.1"
                     /translation="MIKHILRHVETMARAVAEGASKVDGAEVVVKRVPETMPPQLFEK
                     AGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLA
                     SVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPYGATTIA
                     GGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            6279..6506
                     /gene="yccJ"
                     /locus_tag="ACHJ69_004283"
     CDS             6279..6506
                     /gene="yccJ"
                     /locus_tag="ACHJ69_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO5017137.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(6544..7785)
                     /gene="agp"
                     /locus_tag="ACHJ69_004284"
     CDS             complement(6544..7785)
                     /gene="agp"
                     /locus_tag="ACHJ69_004284"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO5017138.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAVMENELSKLQLTDSYKLLEQIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSGEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(8066..8167)
                     /locus_tag="ACHJ69_004285"
                     /pseudo
     CDS             complement(8066..8167)
                     /locus_tag="ACHJ69_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8320..9240
                     /gene="cbpA"
                     /locus_tag="ACHJ69_004286"
     CDS             8320..9240
                     /gene="cbpA"
                     /locus_tag="ACHJ69_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO5017139.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            9240..9545
                     /gene="cbpM"
                     /locus_tag="ACHJ69_004287"
     CDS             9240..9545
                     /gene="cbpM"
                     /locus_tag="ACHJ69_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO5017140.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPSEIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
BASE COUNT         2640 a         2379 c         2422 g         2185 t
ORIGIN      
        1 ggacgatgcc ggatttgacg aagaggattt taaaaaaccc atccgcgtcc atttacccgt
       61 tgtgaatgac cttccaccgg aaggcgtgtt tgatactgaa ttctgcaacc gctatgaaaa
      121 aggcggtaac gatggcatca ccatgatggc tatccccttc gatgacaaca tcaacggtga
      181 agatgccaca actgctggcg atgacaacga taacctggac ggaactattc cggatgatgt
      241 ggagaaaagc gaatccccgg acagcgacga tgactgttct gagtgtgaaa ttcccgtcgc
      301 cactctgagc cttactcatc gcttccttca cctcttctta ttcagcaaag atgaagatgg
      361 aaaataccgg catcatgcca caccagaaca acgcaataac gtgatccgta tggagatgga
      421 cacagaggac agttaccttc agagcctgct tactgctgtg cgcgccgcgc atcatgaact
      481 ggataaactg acgaactatc accttagtcg cctggctgaa tctgtaggga aagcattccc
      541 ccactctgca aatcatcgca tcagcccggc tgaattcgac aagttcattt ccacctggat
      601 gaaaactgac taccttgatc agggcctgct gacaaaagaa tggcagaacg gaaattatgt
      661 ttcaggcatt acccgtacgc cttccggtgc taacgctggc ggcggaaata ttaccgatcg
      721 tggtgaagga ttcaaacatg ataagacatc actcgcacga gatgtagcca ccggcgttct
      781 ggcccgttca atggatgtgg atatttataa cctgcaccca gcacacgcaa aacgcgttga
      841 agaaatcgtg tcagagaata agccgccctt ttctgttttt cgcgacaaat ttatcgccat
      901 gcccggtggg ctggattatt cccgcgccat tgtggtggct tccgtgaaag aagcaccaat
      961 cggcattgag gccatcccgg cacgcgtgac tgaatatctc aacaaagtgt tgaccgaaac
     1021 cgatcacgct aacccggatc cggaaatcgt ggaaattgcc tgcggtcgct catcagcacc
     1081 gatgccgcag cgcggaacag cagaaggaaa acatggcgat gaagaaaagc aacaagcatc
     1141 ggacacaatg gctaatgaac aggcagcgcc tgaatcagtg gaagaaattc cagttaaaca
     1201 taatgaggac acgcaatcac tggaaaatgt ctcatctgta gaaacgaaat accaggaact
     1261 gagggaggaa ctcaataaag ccagggaaaa catcccccca aaaaatccag tcgatgccga
     1321 caaattactg gctgcctcgc gtggagaatt cgttgaaggc atcagtaacc ctgctgatcc
     1381 gaagtgggtg aaggggatcc agactcgcga cactgaggac cagaatcagt ccaaagtgga
     1441 acaaattgcc ccagaagcgg gacaaaacag cccggatacg caacaaaacg ggccagaaga
     1501 gcaacagcca gggccagtag cgcaaccgga gctggaaaaa aactgccgcg tctgtggtca
     1561 gactggcggg ggtaactgcc ctgactgtag tgcggtaatg ggcgatagca cttacacaga
     1621 aacttttgga gaaaatgacg ccgctgatgg agaagactca gcacaaactg aggagaagat
     1681 cattcaggaa aactctgttg atgccgctca ggagggcgaa accgttgttc agaacgagcc
     1741 aggcagtgat acgtccggcg atgacgccaa ttctgagcca gtaactctcg actggaaaag
     1801 acagctcgtg attgccgccg tctatggttt gtgcgccaac cccgcatgta tagccacagc
     1861 gccagcaatc cctgatatcg ccatcatgat tgccaacagg cttgaaaatt tcggaggtga
     1921 taaatcatga atgcctggct tatccccgat cgcattgaag agcagtcatg ggcacgacac
     1981 taccagcaaa ttgcccgtga agaaactgaa gctgagctgg cagacgacct agaaaaaggt
     2041 ctgccccaac acctgtttga atcgctatgc atcgataatc tgcaacgtca cggggccagc
     2101 aaaaaagcta tttcccgtgc atttgatgac gatgtcgatt ttcaggaacg catggcagaa
     2161 cacatccgct acatggctga aaccatcgcc cgtcaccaaa ttaatattga ttcagaggta
     2221 taaaacggat gagtacagca ctcgcaacgc tggcagggaa gctggctgaa cgtgtcggca
     2281 tggattctgt cgacccacag gaactaatca ccactcttcg ccagacagca tttaaaggtg
     2341 atgccagcga tgcgcaattt atcgcattgt tgatcgtcgc caaccagtac ggccttaatc
     2401 cctggacgaa agaaatttac gccttccctg acaagcagaa cggcatcgtt ccggtggttg
     2461 gcgttgatgg ctggtcccgc attatcaatg aaaaccagca gtttgatggc atggactttg
     2521 agcaggacaa tgagtcctgt acatgccgga tttaccgcaa agatcgcaat cacccgatct
     2581 gcgttaccga gtggatggat gaatgtcgcc gcgcaccatt caaaacccgc gaaggcagag
     2641 aaatcaccgg accgtggcag tcgcatccca aacggatgtt acggcacaaa gccatgattc
     2701 agtgtgctcg cctggccttc ggatttgctg gcatctatga caaggatgaa gccgagcgta
     2761 ttgtcgaaaa tactgcatat actacagaac gtcagccgga acgcgacatc accccggtta
     2821 acgaagaaac catgtcggaa attaacgccc ttcttacttc catggaaaaa acgtgggatg
     2881 acgacctgtt gccgctctgt tcccagattt ttcgccgcaa catttacaca tcttcagaac
     2941 taacacaggc tgaagctgtg aaggttcttg gattcctgaa acagaaagtc acagagcaga
     3001 aggtagcagc atgacaccgg acattatcct gcaacgtaca gggatcgacg tgagagctgt
     3061 cgaacagggg gatgatgcgt ggcacaaatt acggctcggc gtcatcaccg cttcagaagt
     3121 tcacaacgtg atagcaaaac cccgctcagg aaagaaatgg cctgacatga aaatgtccta
     3181 cttccacacc ctgctggctg aggtttgcac cggtgtggct ccggaagtta acgctaaagc
     3241 gctggcctgg ggaaaacagt acgagaacga cgccagagcc ctctttgagt tcacttccgg
     3301 cgtgaatgtt actgaatccc cgatcatcta tcgcgacgaa agtatgcgca ccgcctgctc
     3361 tcccgatggt ttatgcagtg acggcaacgg ccttgaactg aaatgcccgt ttacctcccg
     3421 ggatttcatg aaattccggc tcggtggttt cgaggccata aagtcggctt acatggccca
     3481 ggtgcagtac agcatgtggg tgacgcgaaa agatgcctgg tactttgcca actatgaccc
     3541 gcgtatgaag cgtgaaggcc tgcattatgt cgtggtcgaa cgggatgaaa aatacatgga
     3601 ggtttttgac gaaatggtac cggagttcat cgaaaaaatg gacgaggcac tggctgaaat
     3661 tggttttgta tttggggagc aatggcgata gccgtagcaa cgaggtgcaa atgatatgac
     3721 aattaaggaa tgctacctgt gttcgttctg atccagcgcg ggcagtcctt cgtcgacgcc
     3781 aacaactatc cggttcaagt ctgcaaggta acgctgacac aggtgatctt ccgaaggctg
     3841 gatgggaaaa ccagagccgc atcaattaac tcatttaatg cagaatttga gcgaatcgat
     3901 cacaacgaac tacatatgat taaagcggaa attgagaagg aaaagcatat tgccagcctt
     3961 cgaaaaatgc gccgcacatc aatcaactga caaccgcctt cgggcggttt tttcttacat
     4021 atgcggaggt aacttatgcg cgagttcgta aaccaacata gccatggcat tcaaccagtc
     4081 atcacacctg ttgtacagat aaatgcgaat gaatgggtaa cactggagct tttaatggct
     4141 gtaacaggcc tgagaaaagg aaccatatta cgcgccaggg acgctgcgtg gatgaacggc
     4201 agagaatata aacaaatcgc ccccgacgga acgccaaaga aaaacagcga atgtctctat
     4261 catcttccta ccatcaacac ctggattaaa aaccaaccct taccatctca ggatgtttaa
     4321 ttcttgtcca taagagtata acctgagcgt gctcttggac gcaggaggaa caatggcgaa
     4381 ttcagcctat ccagccggcg ttgaaaatca cggaggaaaa ctccgaataa cgtttaagta
     4441 caggggtaaa cgagtgcgcg aaaatcttcg cgtgcccgat acaccgaaaa acagaaagat
     4501 cgctggtgag ttaagggctt cggtctgctt tgcaatcaga acaggaacgt ttgattatgc
     4561 cgagcgattc cctgactcac ctaacctgaa gctatttggc ctggtaaaaa aagatatcac
     4621 cgtcggtgaa ctggcacaga aatggcttac tctgaaagca atggaaatca gtagtaacgc
     4681 cttaaatcgt tatcaatcag tgatgaaaaa tatgctaccg aggcttggcg tgggtagact
     4741 ggcatcctcg attacaaaag aggatttgct gttcatcagg aaagatttac tgactgggga
     4801 aaaggaaagc aggaaaaaca cttccaccaa caaaggaaga acagtcccca cggtaaacta
     4861 ttacatgaca acaactgccg ggatgttcag ctttgccgct gaaaacgggt atctggagaa
     4921 aaacccgttt aattcaataa caccactgag gaaatcaaaa cctgtgccag atccactgac
     4981 cagagaggag tttggtcgcc tcattgatgc ctgtcatcat caacagacca aaaacctctg
     5041 gacagtggct gtttttacgg ggatgcgaca tggtgaaatt gccgcacttg catgggagga
     5101 tatcgacctg aaagttggca cgataacagt gcgaagaaat tttacaaaaa taggtgattt
     5161 tacgctacca aagactgatg ctggcaccga ccgggttata catcttctgg caccagccat
     5221 tgaagcactt aaaaaccagg cgatgcttac tcgtcttggc aggcagcatc agatcaccgt
     5281 taaattacgc gagtacggaa gaacaatttt gcacgagtgc acttttgttt tctgtccgca
     5341 aatcgttcgc aagaatcaca aggcgggtat taactatgcg gtaagctcca tcggggcaac
     5401 atgggactca gcaatgaaaa gagcgggtat aagagcccgg aaagcatatc agtcacgcca
     5461 tacttatgcg tgctgggctt tatctgccgg agcaaacccg acatttattg catcacagat
     5521 ggggcactcc agcgccagca tggtctacaa tgtttatggt gcatggatgc ctgagtgcag
     5581 cgtgactcag gttgctatgc tgaataatgt ccttaatgcc cgtgccccag acgtgcccca
     5641 aagtgaccag gaggatgaaa taaaattata tttttcaaaa tgataaagca tatccttaga
     5701 catgttgaaa cgatggcacg cgcagttgct gagggtgcaa gcaaagtgga tggcgcagaa
     5761 gttgtcgtta agcgtgtacc ggaaaccatg ccgccgcaat tatttgaaaa agcaggcggt
     5821 aaaacgcaaa ctgcaccggt tgcaaccccg caagaactgg ccgattacga cgccattatt
     5881 tttggtacac ctacccgctt tggtaacatg tccggtcaaa tgcgtacctt cctcgaccag
     5941 acgggcggcc tgtgggcttc cggcgcacta tacggaaaac tggcgagcgt ctttagttcc
     6001 accggtactg gcggcggtca ggaacaaacc atcacatcca cctggacgac ccttgcgcat
     6061 cacggcatgg tgattgtccc cattggctac gcagcgcagg aattatttga cgtttcacag
     6121 gttcgcggcg gtacgccgta cggcgcaacc accatcgcag gcggtgacgg ttcacgccag
     6181 cccagccagg aagaactgtc tattgctcgt tatcaagggg aatatgtcgc aggtctggca
     6241 gttaaactta acggctaatc ttcaacagga ggatacgcat gccaactcaa gaagcgaaag
     6301 ctcatcacgt cggtgaatgg gcatctctgc gcaatacgtc gccggaaata gccgaggcca
     6361 ttttcgaagt cgccgggtac gacgaaaaaa tggcggaaaa gatttgggaa gaaggtagcg
     6421 atgaagtgtt agtcaaagcc tttgctaaga ccgataaaga ttcgcttttt tggggcgaac
     6481 agaccatcga acgtaaaaac gtttaaccag cgactccccc gcttcacgcg ggggagtttt
     6541 ctgttatttc accgcttcat tcaatacgct atcaaactta tccattgggc aaaaaccgtt
     6601 ggcatctatc gggcaaccgc ttaattccag cgtcacgcgc tgcgcaggtg cctgcagggt
     6661 taacgcatcg gcattacgta actgttcccc actctggtac acatattcaa ttttcatcag
     6721 gtcgcgattg gctttgctgt catgccaacg ctggaaaacg attttgccgc caatcggcgt
     6781 gcgttcgttc tggtcatgca actgatacgg tttgaaatcc agcgccgtta acagagaggc
     6841 aatgttggag tcgtgcccaa ctaataccgt gattttcggt gcactggtgc gatcggtgac
     6901 cagggctttg tcgatataac tgaccagcgg tttcgcaaca ttgcgcgcca cttccggtga
     6961 ggtaaacagg ctgtcctggt aaccgttttt cagcttcgac aacaccttcc actgctggtc
     7021 agatttgatt tctccccagg ccacctgatc catcggaaaa ccttcgtagt attgcaaagt
     7081 aaacgcatct accagcgagt tgccgacttt cagcggcccg gaaacgcccg gttcttgttg
     7141 atacttcgcg ctaaaggtat ttttgccatc tacaagcgaa cattgttgct tctctttaca
     7201 cgcaggagaa tctttatagt taacgatttg ttccagtagc ttgtagctgt cggtaagctg
     7261 gagtttgctg agctcgttct ccattaccgc caccgcctgt tcactgaatg cggcggaatc
     7321 atcggtgatc actgggttaa aggtggggtc catagtgccc attttttcct ggtgatgaac
     7381 aggaatatca cacccaggga atgcgccagt aataaagaac tgcgcggtcg cgacggtgcg
     7441 ttgcagacta ttggcatagg cataaacggt gtccggtggc gggcattccc ctgatttcac
     7501 catcccctgt tctgccagcc attcacgcat ataatggccc atatacactt cgagcacgcc
     7561 ccctttagtg gtgagctgcc caccggggac gtcccattca ggccatttat tcggcgtcga
     7621 ctgctccagc acactgccat tgttcgccag cggcgcacgt aagttatggc ggctcatcat
     7681 gagcacttgc tgtagctgat agccttccgg tacggtttgt gcctgagcat ttgaagcgag
     7741 taaaactatc cctgctacag ttgcggcgat tagcgttttg ttcattcctc acacctcttt
     7801 tgttatcaat aattaaagaa tgtgacagaa atatgtcatt tttccgggaa caggttcgca
     7861 aaatggctcg ttgaacgcct gtttttctat attccccttt aaaaacaggc aagttttgac
     7921 gcaaactatg cctgattagc cccggcctcg tttacgccgc taatgattac atcagtagct
     7981 ataaagaaaa tcagcggcgt taccattacc aataaaaaaa gaatgtgaga ctaatagaaa
     8041 tggtttttag cacatgtctt tatcattaaa ttgcataacg atttaaatag tcgagttgtg
     8101 cattcctttc cttattgttt aacttgtaag catgagccaa aagttcagga gtgaggtaaa
     8161 taaaatatgt tcctcccacc ttgaggcgac ctcccctccg cgatgatttc aataacatat
     8221 tctgtgttgg catatgaaat tttgaggatt accctacact tataggagtt accttacagg
     8281 ggttccttca atttgtgttg atttacgcga gataacgcta tggaattaaa ggattattac
     8341 gccatcatgg gcgtgaaacc gacggacgat ctcaagacaa tcaagaccgc ctatcgtcga
     8401 ctggcccgca aataccatcc tgatgtcagc aaagaacctg atgccgaagc ccgcttcaaa
     8461 gaggtcgctg aagcctggga agtgttaagt gatgaacaac gtcgcgctga gtatgatcag
     8521 atgtggcaac atcgcaacga tccgcaattt aaccgtcagt tccatcatgg cgacggtcag
     8581 agttttaacg ccgaagattt tgacgatatc ttctcgtcaa ttttcggtca gcatgcccgc
     8641 cagagccgtc aacgccccgc cacacgcggc cacgatattg aaatcgaagt ggcggtattc
     8701 ctcgaagaaa cgcttactga gcataagcgt accatcagct ataacctgcc ggtttataac
     8761 gcctttggca tgatcgaaca ggaaatcccg aaaacgctga atgtgaagat cccggcgggc
     8821 gtcggcaatg gtcaacgcat ccgcctgaaa ggccagggga cgccgggcga aaacggcggt
     8881 ccaaatggcg acctgtggct ggtgattcac attgcgccac atccgctgtt tgatattgtc
     8941 ggccaggatc tggaaatagt ggtgccggtt agcccgtggg aagcggcgct gggtactaaa
     9001 gtcaccgttc caacactgaa agaaagcatt ttgctgacta tcccgcctgg cagccaggcc
     9061 gggcaacgat tgcgcgttaa aggcaaaggt ctggtgagca aaaaacagac cggcgatctg
     9121 tatgcggtac tgaaaatcgt gatgccgccg aaaccggatg aaaacactgc cgcgctgtgg
     9181 cagcaactgg cagacgccca gtcgtctttt gatccacgta aagattgggg gaaagcataa
     9241 tggctaatgt tacggtgact tttactatta ccgaattttg cctgcatacc ggcatctctg
     9301 aagaggagtt gaatgaaatt gtcggtttgg gggtggttga accgagtgag atccaggaga
     9361 caacctgggt atttgacgac catgccgcca ttgtggtgca acgcgcggta cgcctgcgtc
     9421 atgaactggc tctggactgg ccggggatcg cggtggcgct gacgttaatg gatgatattg
     9481 cgcacctgaa gcaggaaaac cgcctgctgc gccagcggct ttcccggttt gtggctcacc
     9541 cgtgagtttg ttgcctgatg cgacgcttaa cgcgtcttat caggcccaca aaaagcactg
     9601 ttcccgtagg ccggataagg cgttca
//
LOCUS       DAZFFE010000144         9408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZFFE010000144 DAZFFE010000000
VERSION     DAZFFE010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.144"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(252..1616)
                     /gene="tdcG"
                     /locus_tag="ACHJ69_004288"
     CDS             complement(252..1616)
                     /gene="tdcG"
                     /locus_tag="ACHJ69_004288"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO5017141.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     YDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(1688..2077)
                     /locus_tag="ACHJ69_004289"
     CDS             complement(1688..2077)
                     /locus_tag="ACHJ69_004289"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO5017142.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(2091..4385)
                     /gene="tdcE"
                     /locus_tag="ACHJ69_004290"
     CDS             complement(2091..4385)
                     /gene="tdcE"
                     /locus_tag="ACHJ69_004290"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO5017143.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPLDFDTNIATTITAHDAGYIDQPLEKIV
                     GLQTDAPLKRALHPFGGINMIRSSFHAYGREMDSEFEYIFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRRALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGMDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPIRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(4419..5627)
                     /gene="tdcD"
                     /locus_tag="ACHJ69_004291"
     CDS             complement(4419..5627)
                     /gene="tdcD"
                     /locus_tag="ACHJ69_004291"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO5017144.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLNASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDNVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5653..6984)
                     /gene="tdcC"
                     /locus_tag="ACHJ69_004292"
     CDS             complement(5653..6984)
                     /gene="tdcC"
                     /locus_tag="ACHJ69_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO5017145.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(7006..7995)
                     /gene="tdcB"
                     /locus_tag="ACHJ69_004293"
     CDS             complement(7006..7995)
                     /gene="tdcB"
                     /locus_tag="ACHJ69_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO5017146.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(8094..9032)
                     /gene="tdcA"
                     /locus_tag="ACHJ69_004294"
     CDS             complement(8094..9032)
                     /gene="tdcA"
                     /locus_tag="ACHJ69_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO5017147.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVD"
BASE COUNT         2519 a         2402 c         2155 g         2332 t
ORIGIN      
        1 ggcttattat ttttttccga gccgcatcaa ggcgatatgc ggtctacgtt tttgcaggcg
       61 agttattaga ataaagaaat gatacgcgcg aggtcagaga aaatcttcac aatcaagact
      121 ttgaaataac ggagatggat aagaattttc tactaaatta atcgcaagag aatattcatt
      181 ctctatttgc ggcagatcac aaaaaaaaag gtgcacattt gtgcacccaa ggatgaaagc
      241 tgacagcaat gtcagccgca gaccacttta atagccagtc ctccgcgtga tgtttcgcgg
      301 tatttatcgt tcatatcttt gccggtttca tacatcgtct cgatcacttt atcgagtgaa
      361 acacgcggtg ccgaggtgcg gcgcatcgcc atccgcgcgg cgtttactgc tttcacggca
      421 ttaatagcat tacgttcaat gcacgggatt tgtacctgtc cggcaaccgg atcgcaggtc
      481 agcccaaggt tatgctccat cgcgatttcc gccgcattgc atacctgcgc cggactgccg
      541 cccagtagtt cagttaaccc tgccgccgcc attgaacagg ccacgccaat ctccccctga
      601 cagccgactt ccgcgccaga gatggaggcg ttcattttat acagcgcgcc aattgccccc
      661 gctgccagaa aatagcgggc aattgaccgc tcgtttaccg gacgacggaa cttatcgtaa
      721 taagccagca ctgccggaat aatgccgcac gcaccgttag tcggtgccgt taccacgcgc
      781 ccgccagctg cgttttcttc actaaccgcc agcgcgtaca tgttgatcca gtcgatgaca
      841 ttcatcggat cgttagagat gttatcgctg gaaaccagtt gacgacgcag cgctacggca
      901 cggcgcggca cattgagcgg accaggcagc acgccttcag tattcattcc acgttcaata
      961 ccgtcatgca tcacttgcca gatacgggca aaccctgcgt caatttccgc tttactgcgc
     1021 agcgccagct cgttgtgcat catcaggcca gatatagaca ggccgttgta atcacacatt
     1081 ttcagcagtt cacctgctga gtggaaatcg taaggtacgg gtgtttcgac atcatacgac
     1141 aggccgaagt gttcttcttc gacaataaac ccgccgccga cagagtaata ggttttgctt
     1201 aatagctctt cctgtccctt ccaggcagtg atccgcattc cgttctcatg gcgaggcaac
     1261 atttcgggat ggaagataat gttctttgct acaggaaaat caacaatatg cgcgccagaa
     1321 gccaccggca accgcccgct gcgcgtcact aactctataa atgcagggat ctcatcaatg
     1381 acaacatcct gcggactgtt tcctgccagt cccatgatga tggcgacatc cgtggcatgg
     1441 cctttgcccg tcagtgacaa cgacccgtac agatcgacca caatatggct cgtcgcggtt
     1501 aataagccgc tactttccag ccgatcaata aaactttttc cggcattcat tggccccaca
     1561 gtatgcgaac tggagggacc aatcccaatt ttgaaaatat cgaatgcact aatcatatcc
     1621 acaccctcgg attgccgttc agtaaagtgg agcggaacga ccttgcgacc gtcccgctca
     1681 cgaggcctta cgcactacgt actgcgatgg cttcaatttc caacttcaca tctttcggca
     1741 aacgcgcgac ctgcacacag ctccgggtcg ggtaggtcgc ctgatgttca tcgaagaact
     1801 gcttatagac ttcattgatg gtggcaaaat cattcagatc ggtgatgaac acggtcatct
     1861 tgatgatatc gcccacactc agcccggcag caaccacgat cgctttgacg ttttcgaggc
     1921 ttaaacgcgc ctgatcttgc acatcagccg ggatctcacc ggtctgtggg caaaccggaa
     1981 tttgcccgga ggtgaagacc atgctgccta aatcaacgcc ctgaacataa gggccgattg
     2041 cgcctggggc acgttgcgtt tcgataatct ttttcataca tcctccggcg tcagagcgcc
     2101 tgggtaaagg tacgtgaaat aacatcctgt tgctgttcac gggtcagtgc gttgaagcgc
     2161 acggcgtagc cagagacacg gattgtcagg ttaggatatt tttccgggtg ctcgatggca
     2221 tccagcagca tttcccgatt cattacgttg acgttgaggt gttgaccgcc ttcgacatcc
     2281 gcttcgtggt ggaaataccc atccagcagg ccgacaaggt tcgttttacg tatcggatct
     2341 tctttgccca gcgccgcagg gacgatggag aaggtgtacg agatcccatc tttggcgtag
     2401 gtgaatggca gtttcgccac cgacgtcaat gaggctacgg cgcctttgcg atcacgaccg
     2461 tgcatcgggt tagcgcccgg cgcgaacggt gttccggcgc ggcgaccgtc cggcgtgttc
     2521 ccggttttct gaccgtacac cacgttagag gtgatagtca gaatcgattg ggtcggcaca
     2581 gcattgcgat aggttggcaa agctttaatt ttcttcataa agcgttcaac cagatcgcag
     2641 gcaatgctgt ctacgcgctc gtcgttgttg ccatactgcg gatattcgcc gtcaatctcg
     2701 aaatccaccg ccaggccgtt ttcatcacgg attggtttca cacgagcata tttgatggca
     2761 gacagggagt ccgttgccac cgacaggccc gcgatgccgc atgccatggt gcgatagaca
     2821 tcacgatcat gtagcgccat cagcgaagct tcgtagctgt acttgtcgtg catgtagtga
     2881 atgatattca gcgcgctgat gtactgtacc gccagccagt ccatgaagtg gtcgaggcta
     2941 tccatcactt tgtcgtagtc cagcacgtca tccatcaacg gagccgtttt cgggccaacc
     3001 tgaatcttca gcttctcgtc cattccgccg ttgattgcgt acagtagcgt tttcgccagg
     3061 ttagcgcgcg caccgaagaa ctgcatctgc ttaccaatca ccattgggct gacgcaacag
     3121 gcaatcgcgt aatcgtcgct attgaagtca gtacgcatca gatcgtcgtt ttcatactgc
     3181 aaagaggatg tgacgataga cacctgcgcg gcatattttt tgaaggcaat cggcaaggct
     3241 tccgaccaca gaatagtcag gttaggttcc ggtgcaggcc ccatagtgtg cagggtgtgt
     3301 aaatagcgga aggagttttt ggtcaccagg gttcgaccgt ccagccccat cccgccgatc
     3361 acttccgtcg cccagatcgg gtcgccggag aacagcgaat caaattccgg cgtacgcaag
     3421 aagcgcacca tacggatctt catgatgaag tggtcgatca gttcctgtgc ctgctgctca
     3481 ttgagtacgc ctgctttaaa gtcgcgctca atgtagatat cgaggaacga tgccgtgcgc
     3541 cccagcgaca tcgcgccgcc attttgcgat ttcactgccg ccaggtaagc gaagtagagc
     3601 cactgcaccg cttcctgtgc attctgcgcc ggacgggaga tgtcaaaacc gtatttcgcc
     3661 gccatttctt gaatctgcaa cagcgcacga cgatgttccg ccagctcctc acgcagacgg
     3721 atcgtagctt cgagatcttc gcctttttcc agacgggact ggagatcggc aaactgcagt
     3781 tcgcgttcac gtaccagata actgatgccg taaagcgcca cgcggcggta gtcaccgatg
     3841 atacgcccac gaccataacc atctggtaaa ccggtcagta caccagattt acggcagcgc
     3901 agcatatccg gtgagtaaac atcaaatacg ccctggttat gggttttacg cagatcggta
     3961 aagatgtatt caaattcgct atccatttca cgaccatacg cgtggaatga acttctaatc
     4021 atattaatgc cgccgaacgg atgcagcgca cgctttaatg gcgcatccgt ttgcagaccc
     4081 actatttttt ccagcggctg gtcaatatat cctgcatcat gagcggtaat tgtggtggca
     4141 atattggtat cgaaatccaa tggcgcgtga gtcgcatttt cgatacggat gccttccatg
     4201 actttttccc acaattccgt ggtggcaggc gtcgcttcgg cgagaaaaga ttcatcgcct
     4261 tcataaggtg tatagttatg ttgaataaaa tcgcggacat taatttcgtt tttccagtcc
     4321 gtacctttaa agccaagcca tgcgtcggcg tacagcttat cgctggtatc aatatctacc
     4381 ttcatgaaaa ataatctctc tacaatactt caactaaatt atgcaaattc tgcgggcgcg
     4441 ttcactttgc ctaaatgaat ggcatccaaa gcaatcattt tttcttcgtt agtcggaatt
     4501 acggcgcaaa tgacacgcgc attttcactg gaaacaattc gctctccaaa agagttagag
     4561 cgattattca tttctgtatc aatttctacg cctaatacag ctaaatgttc catgaccaga
     4621 cgacgaatta aacttgaatt ctctcctatt ccgccggtga atataattcc atccaggcga
     4681 tgtaatgaag cagcgtgtcc ggcaatatga cgggcaattc ggtgaacaaa ggttttaatt
     4741 gccagttgcg cgcgttcgtg accttcatgc caggcttttt ccagaacacg taaatccgaa
     4801 gaaaggccgg aaatacctaa taatcccgac tctttattca ctacgcgttc caggtccccc
     4861 aggctctggt tggtttggct ggcgacccag gacatcgccc cgaagtcgac atcaccactg
     4921 cgggtaccca tcatcaagcc ttccagcggc gtcattccca tggaagtgtc aacactctga
     4981 ccgttgcgaa ccgcgcagat tgacgcgcca ttgccaagat gcgccacaac caggccggag
     5041 tcatcttccg ccagattcag cagagaatgc gcgcgctggg aaacatagcg gtgcgacgtg
     5101 ccgtggaaac cataacggcg tacacctaac tcttcataat atttccacgg caggccgtat
     5161 aaataagctt ccggagccat cgtctggtgg aaactggtat caaataccgc cacctgagtt
     5221 acgcccggga ataattgctg cgccgattca ataccactta aattggcgta attatgcagg
     5281 ggtgccagtg gagaaacgcg acggatatta tcaatgactt catcggtaat aatggcggac
     5341 tcggtaaaaa tactgccgcc gtgagcgatg cggtggccaa ttaaggccac attgtcattt
     5401 aaattccgtt tttccagttc aaatgcaatt gccttcaatg caccttcgta gctgtggtga
     5461 gccagcggtg ctggctctcc cccatttacg gataagaatg cattttccga gttaataccg
     5521 tcggcaatac ctgacattaa tacttcacag tcgctggcat tgagtacgga aaacttaatc
     5581 gaagacgaac cacagttaat aaccaaaaca accggaaatt cattcatctc ttttctcatc
     5641 ctgagttacg gattaaaaca gtttgtatac gatgttcagg atggtcagca gaccaatcac
     5701 ggtaacaaac acgttatcca gacgaccacg gtatttcgcc agagacggcg ctttacggat
     5761 ggcatacatc ggcaacaggc acagcagtga tgcgataatc ggtgcgccca tggcttcaat
     5821 caggtcgagg atgttcgggt tggcgtaggc aacaacccag gtggagccca taatgaaaat
     5881 catgctgata gtattcagtt tacccagcga cactttggtt ttgtcacctt tataaccgaa
     5941 cttcagaatc aaaccattca gcccttccag cgtccccaga tagtgaccga agaaagattt
     6001 gaaaatagcc acgagtgcga tgatggaagc ggcatattcc agtgtaatcg caaacgttgt
     6061 tttggtaccg gtcatggacg caaagtggtt agccagataa gaaagcactg gaatattctg
     6121 cgctttcgct tcagccatat tcgccggaga cagcgtaaac aggcagctaa aggcaaagaa
     6181 catcaccact gcaaccatca gcatactggc gcgagagatg atttgtgaac atttacgctc
     6241 ggtgaagtcg cgaccaaaat ctttctcgta ctcttcacgt ttagaaacca cgaaggaaga
     6301 gacgattggc gagaagttaa aggagaaaac catgatggaa atccccagcc agacggtgat
     6361 caggataccg tcatgaccag ttaacgacag cgaaccgagg tcaacctggt cgataactgc
     6421 agagttccag taagggatca gcgaaagaga aatcagcacc aggctggcga taaacggcca
     6481 taccagatag ctcatcactt taaccatcag atccttacca aaccagatga cgaaagccat
     6541 cagcagcaac aggaacagcg ccacaaagcc gcgattcagc ggtgcaaagc cgagctggtt
     6601 ttcccagaac gtcataaagg tattggtaat agtaacgcca taaatccaca acagtgggca
     6661 aatcgcgaag aagtacagga acgtgataac cacgccgcca gttttaccaa aatgctcttc
     6721 caccgtttcc gtaatgttgc cggaagggtt agagccagaa agacacagac gcgccagtgc
     6781 ccggtggcaa taaaacgcga tggggtatgc taataccaac atcagaagaa tcgggatcag
     6841 tccgccaaaa cctgcgcgga tagggaagaa cagcaccccg gcgccgattg ccgtaccaaa
     6901 caagcctaac gtccatgtgg tatctgattt acgccaggac gattgttttg tctggctgga
     6961 tacaatgcta tctgaagtac tcatatccta tcctcaacga attaattaag cgtcaacgaa
     7021 accggtgatt tgagagacgc gagaaagatc gatattgccg ccggaaataa tactgacggt
     7081 ttttctgttt tgaatatatt ggtctaattt accgcttaat aatgcagcac atgccagagc
     7141 gcctgcgcct tcggtgacga ctttattgcg ctgaattaag gcaatcatac tgtttctgat
     7201 ttcgtcttcg ctgaccagca cgatgtcatc gactaattca cgaacgattt cgtaagttaa
     7261 attacccggg cgggagacat cacaaccatc cgccagggtg ccggtagttc ggtgcgtagt
     7321 tatttctccg gagtggaaag aagccgccat gccgtgaacg ttttcagact gtacgccaat
     7381 aacacggatg gtcgggttga tagatttaat tgccaccgca atacctgcaa ttaaaccgcc
     7441 gccgccaatt ggcacaatca cgttatcgac atcatagaga tcttccataa tttccagacc
     7501 aatcgttccc tggccagcaa tcactttcgg atcatcgtaa ggtgggataa aaatacggcc
     7561 ttccatttcg acaatttcgc tcactttagc aatagtgtcg ttgaagttat caccatgcaa
     7621 aacgacttct gcggagtagt cgcacgttgc cgcgactttg gatttcggcg caccttttgg
     7681 catcaccact ttaccgtcaa tacccagcat cgcgcaggag agcgaaaccc cttgcgcatg
     7741 gttgcccgca gagcaggcca ctacaccttt acgtttttcc gcatcagtca gtgaacttaa
     7801 tttattaaat gcgccacgaa ttttaaatga accggtacgc tgcatgtttt cgaatttgag
     7861 gaatatttca cctttgcaac gttcactaaa atagttggag cgaggcatac ctgttttata
     7921 aattcgccca gccagtcgtt gttttgcttc aatgatgtca tcaatagcaa ccgggagatc
     7981 gtatgtaata tgcattataa aacctcttag cctgaattaa atatgtaggt aaccgtaacc
     8041 gacacctgca aaacagacag gtggattatt tatgtttaaa ttaaaacaaa taactaatca
     8101 acttcaatta attgccttcg tctacaccca ttataagatg aatactcttt ggctaattcc
     8161 accaaaaccg atgctgcttt tttaatacga taatttttcg accataccgc ggcatattgt
     8221 gccacaggta atgtttcttc aaccggaata gtaataaatt gattagaacc aaaaggtgat
     8281 gtcatatcac aaggaattac agttaagaaa tcagcattga gaacaagatt ataaattgtc
     8341 acgactgagt cggttttaac gatgttttca atactgatgc catttctttg taacgtagta
     8401 agtagttcgc tgtagtaccc catattggtt tgtggcaaca cccactgttc gttcttcaac
     8461 gactccagcg tggtggtgcc ggtgcatgtt cgggacttac tggctaccag cacaaactcg
     8521 gactcgaaca gcggctcaac atgtaaatcc tgaagcttca tttctgcgct taacgtacca
     8581 atcgcaaaat ccagtcgacc gtcgcggatt gccggtaaga acgaagacag ttgcgcttca
     8641 tacatagaaa cctgcgcttt cgggaacacc tctttgaact tgttgatcat ccctgacata
     8701 aaagtaaaac caatcaatga aggaaaacca aatgagactt ccaccaccgc ctcagaagac
     8761 ataccgctta tctcattaac catatttttc atttcacggg taatggattc ggaacgggag
     8821 agtaacaatt gaccggcagg tgttaatgtt acaccggtat ttttccgcac cactaactcc
     8881 acaccaaaat aatcctcaat atcgttaatg attttactga cggccggttg agttaaccct
     8941 aactcttttg cagccgagcc gatagaacca cttctaataa cttcctgaaa gactaccagg
     9001 tgctgcgttt tcggaagaag aatagtgctc ataacgacct acgttaatta cctcattgac
     9061 ggcatgaagt gtatcaaaat gaaatgaaca ggatatgtgc gaccactcac aaattaactt
     9121 tcaatacttt ccatggcatc gttaccccaa acaaatacct ttaaatatca acaagttaaa
     9181 atatgaaaat cagcataagc cctgattttt gtcaaaaaaa atgacgggga taaccatatt
     9241 ttatggcgat aacatcattc gttatgtggt taatacaaaa aaggggatga gactatattt
     9301 caagcaatta ccggataatt accaggcaaa atttgagtta aatttaatga gtaagttctc
     9361 aaattttatt ataaacaaac cattttcagc gataaataat gcggcacg
//
LOCUS       DAZFFE010000145         9232 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFFE010000145 DAZFFE010000000
VERSION     DAZFFE010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.145"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..397
                     /locus_tag="ACHJ69_004295"
     CDS             <1..397
                     /locus_tag="ACHJ69_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HFO5017148.1"
                     /translation="LDSHHHVHMFPQIFPIVAKFAAEEGIALRIDRQPLSNDGDLPAN
                     LRSSQGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSAYCFPR
                     LTELHVLTSASLKYAIAERGYRLGSYHDV"
     gene            complement(444..2705)
                     /gene="katE"
                     /locus_tag="ACHJ69_004296"
     CDS             complement(444..2705)
                     /gene="katE"
                     /locus_tag="ACHJ69_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO5017149.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDTNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQVAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQHHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIANNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIAEADSADGSFMDELLTLMTAHR
                     VWSRIPKIDKIPA"
     gene            2888..3151
                     /gene="cedA"
                     /locus_tag="ACHJ69_004297"
     CDS             2888..3151
                     /gene="cedA"
                     /locus_tag="ACHJ69_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO5017150.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQENEVEE"
     gene            complement(3198..4589)
                     /gene="tcyP"
                     /locus_tag="ACHJ69_004298"
     CDS             complement(3198..4589)
                     /gene="tcyP"
                     /locus_tag="ACHJ69_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO5017151.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSKDDAELAHR"
     gene            complement(4722..5312)
                     /gene="ydjM"
                     /locus_tag="ACHJ69_004299"
     CDS             complement(4722..5312)
                     /gene="ydjM"
                     /locus_tag="ACHJ69_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5017152.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVSQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(5476..6144)
                     /gene="hxpB"
                     /locus_tag="ACHJ69_004300"
     CDS             complement(5476..6144)
                     /gene="hxpB"
                     /locus_tag="ACHJ69_004300"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO5017153.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVEQVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFNLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6291..6827
                     /gene="yniB"
                     /locus_tag="ACHJ69_004301"
     CDS             6291..6827
                     /gene="yniB"
                     /locus_tag="ACHJ69_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO5017154.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNVFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PLICIAAGYVFFSLLGFI"
     gene            complement(6868..7728)
                     /gene="yniA"
                     /locus_tag="ACHJ69_004302"
     CDS             complement(6868..7728)
                     /gene="yniA"
                     /locus_tag="ACHJ69_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO5017155.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGRDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     DKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(7834..8124)
                     /gene="ghoS"
                     /locus_tag="ACHJ69_004303"
     CDS             complement(7834..8124)
                     /gene="ghoS"
                     /locus_tag="ACHJ69_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO5017156.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(8225..9154)
                     /gene="pfkB"
                     /locus_tag="ACHJ69_004304"
     CDS             complement(8225..9154)
                     /gene="pfkB"
                     /locus_tag="ACHJ69_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO5017157.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHGDTQKIYAYLSR"
BASE COUNT         2303 a         2291 c         2338 g         2300 t
ORIGIN      
        1 tcttgatagc catcatcatg tgcatatgtt cccgcagatt ttcccgattg tggcaaagtt
       61 tgcggctgaa gaggggattg cgttgcgcat cgaccgtcaa ccgctatcta acgatggtga
      121 tttaccggct aatctgcgca gttcgcaggg attcagtagc gcgttttatg gtgaagagat
      181 tagcgaggca ttgttcctgc aagtgcttga tgattccagc catcgtgggg aacgctcgtt
      241 ggaagttatg tgccatccgg cgtttgtcga taatacgatt cgccagagtg cttattgctt
      301 ccctcggtta acggaactgc atgtgctgac ttcagcgtcg ttgaaatatg cgattgctga
      361 gcgtggttat cggttgggta gttatcatga tgtgtaagta aggtggtaag gtcactcccc
      421 agggggcgtg accttatcca tatttacgcc gggattttgt caatcttagg aatgcgtgac
      481 cacacgcggt gtgctgtcat cagcgttaac agttcatcca taaaactacc atcggcgctg
      541 tcagcttccg caatcccttc ttcaccctgg tcagcgacct tgattgttgc tttaaacttg
      601 cgcgcgtctc ctgccagcgc aatcggttta aggtgtttgt aggcttccat cagatagtag
      661 ttggcatcgc cgttgttagc gatatccgca atattgccgc aagggacaat gactgcatcg
      721 accgtcagcg aaggcgcacc ggcaaaggta gcagctatag gcaacaccgt accgtcatcc
      781 gcagtcactt cccccattcg ggagtagagc agtttggcat gaacgccttt ggccttcagt
      841 gccttgagaa tggccagaag gtctgccgat ctcacttcat cattaagcaa aattgctacc
      901 acgcgacctt tcacatcacc gtcaggaatg gcgtacagac ttaaggatgg atcctttttc
      961 agaccgttga cgtccggagg cggggtgata ttcaactgat cgtcagtcag ttcgataccg
     1021 agatttttcg ccaccgcctg ggccagagtg agatcaatat gcgccagctg gtcaacaacg
     1081 cgctcacgaa tatacggacg aactactttg cttaactcaa aactgaaacc atcgacaata
     1141 tggtgctgct cgaatggcgt ctgacttagc cagaacagac gcggatggga ataatattcg
     1201 ccaaacgatg gactgcgctc gcgaacttta ttcccttcca cgcgttcctg gtatgattca
     1261 aaaccgccgc gtttcggccc cggcggtgtt tcgcgcggcc agttatcgtt gatcgagttc
     1321 ggttcgtaat tcgccgggtt agtgtcgatc cccatacgat gcatgccgtc acgctggaaa
     1381 ttatggtaag ggcaggtcgg gcggttaatc ggaatttcat ggaaattcgg cccaccaaga
     1441 cgactgattt gtgtatcggt ataagagaac aaacgcccct gcaataacgg gtcgttggtg
     1501 aaatccagac cggggacaat atgccctgga tggaaagcca cctgttcgtt ttcagcaaag
     1561 aagttatccg gattacgatt gagcaccatt ttgccgacac gctgaacagg caccaattct
     1621 tccgggataa gtttggttgg atcgagaaga tcgaagtcga acttgaattc gtcttcttca
     1681 ggaatcaact ggaagcccag ttcgtattca ggaaaatcgc ctgcttcaat cgcttcccac
     1741 aactcgcggc ggtggaaatc agggtcacgt ccggttaatt tttgtgcttc atcccaaacg
     1801 agtgaggctt tacctgccag tggtttccag tggaaacgta cgaacgttgc cttcccttcg
     1861 gcattaatca ggcggaaggt atgaataccg aagccttcca tggtgcggta actgcgcggg
     1921 ataccgcgat ccgacatcgc ccacatcacg ttgtgcagag tttcaggttg cagagaaaca
     1981 taatcccaga aagtatcgtg ggcgctttgc ccttgtggaa ttgcccagtg cggttctggt
     2041 tttaccgcat gaacaaaatc ggggaattta tgcgcatcct ggataaagaa gattggcgtg
     2101 ttattgccaa cgaggtcaaa aataccctct tcagtataga acttggtggc aaagccacgg
     2161 atatcacgca cggtatcggc agagccagcg ccgccctgaa cggtagagaa cctaacaaat
     2221 actggggtga ttttgttcgt atctgagagg aaatctgctt tggtgatatc gcttaagctt
     2281 ttatatggct ggaaataacc gtgagctgct gatccgcgtg catgaacaat acgttccggg
     2341 atgcgctcat ggtcaaagtg agtgattttc tcgcgcagaa taaaatcttc cagcagagtt
     2401 ggaccacggc taccggcacg tagtgagttt tgatcgtcgg cgatgcgcac gccctgatta
     2461 gtggtcagcg cataattttc actgcctttg cgtacgtctt ccagagaatt aagtttttcg
     2521 ttacgcgtat caggggcttt caggctccct ggggcggtag gttgtgcacc aggcggtgtt
     2581 ggttcagccg ctggacgatg agatccgtct tcaggtgcca gtgagtccat ccccggtttc
     2641 gcttcgctgg aatcgtgtag tggtgactgg tgctgatgtg ggttcttttc gttatgttgc
     2701 gacattgaac tcgtctcctt aaattattac tgaaagggcc gctgttttgg tcaaaaaacc
     2761 ctctaactat agaccaccag ccagatcccg cttcggaaac tattctggaa acggtgttat
     2821 gaaatacacg caaagcggga cgtacagggg ctaaatcggc taaactacag tttttctgat
     2881 ttttaatatg cgtttagtga agccagtaat gaagaaaccg ctccgtcagc aaaaccgcca
     2941 gattattagc tatgtcccac gcacggaacc cgcgccgcca gaacatgcga taaagatgga
     3001 ttcgtttcgt gatgtctgga tgctgcgtgg caaatatgtt gcgtttgtac tgatggggga
     3061 gtcatttctg cgctcaccgg cgtttactgt gcctgaatcc gcccagcgtt gggcaaatca
     3121 gattcgtcag gaaaatgagg tcgaagaata aaaaaacacc tgtcgggatg acaggcgttt
     3181 aacgtaacaa atcaggatta acggtgtgcc agttcggcgt cgtctttgct atccagaatg
     3241 gctttatcgg tttgcttcag ccactggctg gtcagcgtgc cagcggtcat cgagccacta
     3301 acgtttagcg ccgtacgacc catgtcgata agcggttcaa cggagattaa caatgccacc
     3361 agcgttaccg gcaggcccat cgcaggcagc acaatcagtg cggcgaaagt tgcaccacca
     3421 ccgacaccgg caacgcctgc ggaactaacg gtaacaatac cgaccagcgt cgcaatccac
     3481 atcgggtcca gcgggttaat gcccaccgta ggcgcaacca tcaccgccag cattgccgga
     3541 tacaaaccgg cgcagccgtt ctgaccaatg gtcgcaccga aagaggcggc gaaactggcg
     3601 atggattcag gaacacccag acgacgcgtt tgtgcttcca cattcagtgg gatagacgca
     3661 gcactggaac ggctggtaaa ggcaaacgtc agtactggcc ataccttacg gaagtacttc
     3721 agcgggctca cgccattaat gcccagcaga aggccatgca ctgcaaacat aatcagcaga
     3781 ccgaggtagg acgcaacaac gaaacttccc agtttgatga tgtcttgcag gttagaacct
     3841 gcaaccactt tggtcatcag cgccagaaca ccgtaagggg tcaactgcat gaccaggcga
     3901 accagtttca tcacccagct ttgcagggta tcgatagcgg ttaagacgcg ttcacctttc
     3961 ggcgcatcat ccttcagcag tttcagcgca gctacgccga ggaatgcggc aaaaattacc
     4021 acgctgataa ttgacgtcgg attggctccg gtcagatcgg caaacgggtt tttcgggata
     4081 aaggacaaga ccagctgcgg aacgctcagg tcagagactt taccaacata gttactttcg
     4141 atggcgttca gacgtgcagt ttctgcacca ccctgaacca gaccttcagc cgtcaaacca
     4201 aacaggttgg tgaccagcac accgaccagc gctgcaatca gcgtggtaaa caaaagcgta
     4261 ccgatggtca gaaaactgat tttgcctaac tgagatgcgt tatgcagacg ggcaaccgcg
     4321 ctcagaatag aggcgaagac tagcggcata acgatcattt gcagcagttg aacatagccg
     4381 ttaccaacaa tgttaaacca ctgtacagaa tctttaagta cctggctgtc agaaccataa
     4441 atggtatgca gggcaaggcc aaaaaccaca cccatcacaa gacccaccag cacttttttc
     4501 gccagactcc actgtttgtg gcgggtttga gccagcgcaa acagcagtac aacgaacacc
     4561 acgatgttcg caattaatgg aaagttcatc cccgttctcc tgatcttatt atttctatca
     4621 ctgcgtaaca gtcatagtgt tggcgcaaga ttaacagaac tgtgagctgg ctcttatatt
     4681 caaatggaat tgcttattcc agaaaggcga tttttgcctt tttagtattc aacctgatgc
     4741 ttaataagcc gatgaaactg gatctgtaag gtattgatca tttgcgatga ccaacgaaca
     4801 gcgctgtttt cgggtaatga atggggcatc cataccgacc agacaaataa tgccatgcag
     4861 ataaaacgtt ccagttggtt gcccttttgg gaaaccagga taggcaagcg gaaacgccag
     4921 cggcatggcc agagcagggg aacaccggcg ggtgtcagca tatcggcaag tatgtggctc
     4981 aaataaccca gcaccatacc ctgcaacgca tcagccggaa tgaaccagct ttcaggaact
     5041 ttaaggtaaa aggttgccag cagcgcaaat accgccagca gactgtgggt aaaaccacga
     5101 tgcccaaaag cgcgggcgat cggttttgat atccacttta atcgctgccc aagaaacgac
     5161 tttgggtgat cgatgtccgg taacaaacac gtcaggattg cggaagggac aatatgccac
     5221 cagtcaccct gtgccagcac gggcgtcagc tcggcatttt tggcaaatac cgcacaagca
     5281 atagaaaaga gaaggtgacc ttccgccgtc atgataaaac tcactaaact gtcgatacgt
     5341 acagtatagg gtttttatac agtgcacgga agtggtgacg gtgtaactct ttctcttaaa
     5401 ttggcgatta atccgtcagt tgaatgcaaa ttcagataaa gaaccagggg cagtgcgatc
     5461 actgccccct ggtcattaac cgagaaggtc ttttgcggtg agttctgtca gcgatgagag
     5521 tttgacgttt gctaatacaa aacgtggatc attttgcgct tctggcgcag gaacgacgat
     5581 ggaacgcatg cgggctgctt tagaggcgat catgccattt accgaatctt ccagtgccac
     5641 gcaggtcaga gggtcaaccc ccagttttgc tgcgcagtcg agatatactt gcggatgcgg
     5701 cttgctgtaa ggcagttttt cagccgaggc gagggcatcg aaactgtcgc gtaagttaaa
     5761 catggtcaac actttttcca gcatatgtag tggtgacgcg gaggccagtc ccaccaataa
     5821 accttgttct ttgcataacg caacggcttc acgcacgcct ggcaataatg ggcgtgtctc
     5881 ttcaaccagt gaaatggcac gggcaataac ttgttctact acttcctgac ggcttggccc
     5941 attccatggt tgccgggcgt accaaagatc gaccaccata tcgatgcgta aacctaaggt
     6001 gtccggcagc tcgttacgac gggagatatc cacccccagg cttgccatca catccagttc
     6061 ggctcgatcc cataaaggtt ctgagtcgat aagtaatcca tccatatcaa aaattgcagc
     6121 aagaatctga cgcggggttg acattacaac ctctccttta gagcattata tgtaaggtat
     6181 taagccgtgc ttatgattaa gcgcctatca tatcgccttt aaagaatggg cgaaaacaga
     6241 tatctacagg taaactaagt gaaacgacgc gtcttaatca aggagaactc atgacgtatc
     6301 aacaagctgg acgcattgct gttttgaaaa ggattttggg ttgggtgatt tttatacctg
     6361 cattgatctc tacattgatt tctttgctga aatttatgaa tacccggcag gaaaaccagg
     6421 aaggcattaa tgcggtcatg ctcgacttta ctcatgtcat gatcgatatg atgcaggcga
     6481 atacgccttt tttgaatgtg ttctggtaca actctccgac gcccaatttt aatggtggcg
     6541 tgaacgttat gttctgggtg attttcatcc tgatttttgt cggactggcg ttgcaggatt
     6601 ccggagcccg gatgagtcgc caggcgcgct tcttgcggga aggtgttgaa gatcaactca
     6661 ttctggaaaa agccaaaggg gaagaggggc taacgcgtga acaaattgag tcacgtattg
     6721 ttgttccgca ccatactatt ttcctgcagt ttttctcgct gtatatcctg ccgcttattt
     6781 gtattgctgc aggttatgtg ttcttttctc tgcttgggtt tatttaatca aaaggccgca
     6841 cggagcggcc attatcctca acccatatca tgctgctaat aatctatcca gtgactgctg
     6901 agcaataacc aaatgctgac cgccaaataa ccttgcacga tttaacagcg tgtagagttg
     6961 gtaaaccggt tgacgttcaa ggaaatcggc aggtagcggg gatactgact gatagccgtc
     7021 atagatttgt ggcggctgtt cagtatgcag cggtaacatc gccaggtcgc actctcggtc
     7081 accccagtaa caggccgggt cgaaaatgta cgggccatcc ggacccagcg cacagttgcc
     7141 ggaccataaa tcgccgtgca acagagaagg ctgcggttga tgcgaggcga gacgctgctg
     7201 aatatgctcg acgatggcat cgatattgcc gaaagcgatc cctttgtctg ccgccagttc
     7261 cagctgccag ccaatccgtt gttcagcaaa aaaagttgac cagcgacgtt gccaggtgtt
     7321 gggctgtgga gttgtggaga gcgcgttatc gaaatcgagg ccaaattgtg gttggtcgct
     7381 ccattgatgt aaacgcgcaa tttgctgacc aagaataaat gcactatgcg catccagcgg
     7441 gcgaggtggg agataatcca tcaccagaaa actgtagtca cggtcagcgc caactgccca
     7501 aaccttaggc acggtgacgg ttttactacg cgacagtaac tccagttggt cggcttcggc
     7561 ggtaaaaccg ggaagcagtt ccctttcatc acatttgacg aaaaagtcac gtcctgcata
     7621 gcgcaaatgc catgcggcat ggacttctcc gccaggcagt tcattacgca gttcgatttc
     7681 gccttcacct aactgctcgc tcaaaagacg actaattgcc tgccacatga tgtctctccc
     7741 atgttgtctg ccagctcata aggttagcgc aagaatgcgg tgtaaaaaca cgagctaacg
     7801 cacaccacag actgattatt gcccaaaaac catttatttt ctgttgctat tccattcctc
     7861 ccaggtcgtg atggtgattt caggatcttt gccggttgca ctttgggtaa gccccgaaac
     7921 tagttctcta atttcttctt cactttgggt actgataagt ccaaaagtgt tagtccccag
     7981 ctcatggata ttgccatcat catccgtcat ggtgagcaaa aaaccgtcgc gagtcaggta
     8041 attattcaac tcgttaattt cagtcaatgt atcctcatgc aacatgacgg ttattacata
     8101 acggacaaga tcgccactgg ccatagttca cccctttgtt atcacatgac ttttgagcat
     8161 agtcggagaa acgcgttgcc gacaggttgg tggtgattcc cccaatgctg ggggaatgtt
     8221 tttgttagcg ggaaaggtaa gcgtaaattt tttgcgtatc gccatgggag cacagacgtg
     8281 ttccctgatt gagtgtggct gcactccccg cagctacgcc aaaacggacc atttcttcaa
     8341 gagaggcatt ttctgccagt ttcagtgtca tcgcgccgac catgctgtca ccagcgccaa
     8401 cggtactctg gcttttcacc ggtggtggca cgacctgaat gcagttttca ctatcaacgc
     8461 ccagcgcccc ttgtggaccc agggaaacga caacccgttt ggccttgccg ctattaacga
     8521 tttcctgcgc ggctttgcgg acatcatctg gctgggtgag ttcgcgattt accagcgcac
     8581 tgagttcttt ttggttaggc ttaaccaact cgatgttacc aatcgccagt gctgcactta
     8641 acgcttcgcc agaactgtcg acgatgcagc ggatcccttg tttttgcgca gcggaaatca
     8701 gttgggttaa tttttccagc ttcacacctg gcggcaggct tccgcttatg accagaatgg
     8761 ccccggattc aatttccaga acttgctctt caagctggcg aaactcatct tcatttaatg
     8821 ccgcgcctgg cataacaaaa cgatactgct caccgcttgc ttccacatgt acgtgtaaat
     8881 tctgccgggt ccagtctttg gcttctacag tagcgacggg gacattttca tctgccaaca
     8941 gtgaaaccag atgttcgccg gtcgcaccac ccgccgggaa gatcgctgtg gcactgcctc
     9001 caagatgggc aatggcgcgg gcgacgttga tgccgccgcc tccgggttcg aataccggtg
     9061 cggtacagcg cagttttcct tcgggataaa tttgcggggt aattgttgcg ctatcgagtg
     9121 agggcgcaag tgtcaacgta tagatacgta ccatcatttc ctcctatagg ctgatttcag
     9181 tctggcaccg aatcagcgga aagtgaagaa attaacaata tgatttattg ga
//
LOCUS       DAZFFE010000146         9168 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFFE010000146 DAZFFE010000000
VERSION     DAZFFE010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.146"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(42..110)
                     /gene="ykiE"
                     /locus_tag="ACHJ69_004305"
     CDS             complement(42..110)
                     /gene="ykiE"
                     /locus_tag="ACHJ69_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFO5017158.1"
                     /translation="MGWSICFWHVSVRMTHDVVCYY"
     gene            247..768
                     /gene="fecI"
                     /locus_tag="ACHJ69_004306"
     CDS             247..768
                     /gene="fecI"
                     /locus_tag="ACHJ69_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFO5017159.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            765..1718
                     /gene="fecR"
                     /locus_tag="ACHJ69_004307"
     CDS             765..1718
                     /gene="fecR"
                     /locus_tag="ACHJ69_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFO5017160.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1805..4129
                     /gene="fecA"
                     /locus_tag="ACHJ69_004308"
     CDS             1805..4129
                     /gene="fecA"
                     /locus_tag="ACHJ69_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFO5017161.1"
                     /translation="MTPLRVFRKTTPLVNTIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLSPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYLNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEIGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            4174..5076
                     /gene="fecB"
                     /locus_tag="ACHJ69_004309"
     CDS             4174..5076
                     /gene="fecB"
                     /locus_tag="ACHJ69_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFO5017162.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5073..6071
                     /gene="fecC"
                     /locus_tag="ACHJ69_004310"
     CDS             5073..6071
                     /gene="fecC"
                     /locus_tag="ACHJ69_004310"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFO5017163.1"
                     /translation="MTAIKHPVLLWGLPIAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6068..7024
                     /gene="fecD"
                     /locus_tag="ACHJ69_004311"
     CDS             6068..7024
                     /gene="fecD"
                     /locus_tag="ACHJ69_004311"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFO5017164.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7025..7792
                     /gene="fecE"
                     /locus_tag="ACHJ69_004312"
     CDS             7025..7792
                     /gene="fecE"
                     /locus_tag="ACHJ69_004312"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFO5017165.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMK"
     gene            complement(8353..8610)
                     /locus_tag="ACHJ69_004313"
     CDS             complement(8353..8610)
                     /locus_tag="ACHJ69_004313"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017166.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(8693..>9168)
                     /locus_tag="ACHJ69_004314"
     CDS             complement(8693..>9168)
                     /locus_tag="ACHJ69_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO5017167.1"
                     /translation="TGKRWAYLAVVPDLFARKPVGWAMSFSPDSRLTMKALEMAWETR
                     GKPVGVMFHSDQGSHYTSRQFRQLLWRYRIRQSMSRRGNCWDNSPMERFFRSLKNEWV
                     PATGYVSFSDAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNAEASFS"
BASE COUNT         1986 a         2624 c         2534 g         2024 t
ORIGIN      
        1 cagcattacc gaaatcccca tcctttaggg cagtgaggat gtcaataata gcagaccaca
       61 tcatgagtca ttctgaccga cacatgccag aagcagatgc tccatcccat tttataccta
      121 ccttataaca cttagaaaaa caacatgtta aaaatgtcta ttggaaacaa ttttatttcc
      181 aattgtaatg ataaccattc tcatattaat atggctacgt gataattaac ttttgatgca
      241 ctccgcatgt ctgaccgcgc cactaccaca gcttccttaa cgttcgagtc gctttatggc
      301 acacatcacg gctggttgaa aagctggctg acgcgcaaac tccagtctgc ttttgatgca
      361 gatgacattg cccaggacac ttttttgcgg gtaatggtca gcgaaacgct ctcgacgatc
      421 cgcgatcctc gctccttcct gtgcactatc gccaaacgcg tgatggtgga cctgtttcgc
      481 cgaaacgcgc tggaaaaagc gtatctggag atgctggcgc ttatgccgga ggggggagcg
      541 ccttcacctg aggaacgcga aagccaactc gagaccctac aactcctcga cagcatgctg
      601 gacgggctaa acggcaaaac acgtgaagcg tttctgcttt cgcaactgga tggtctgaca
      661 tacagcgaga ttgcgcacaa actcggtgtt tccatcagct ccgtgaaaaa atacgtggcg
      721 aaagccgtcg agcactgcct gctgttccgt ctggagtatg ggttatgaat cctttgttaa
      781 ccgattcccg ccgtcaggcg ctgcgttcag cttcccactg gtatgccgtt ctaagcggcg
      841 agcgcgtcag cccacaacag gaagcgcgct ggcaacagtg gtatgaacag gatcaggata
      901 accagtgggc ctggcagcag gttgaaaacc tgcgcaacca gcttggcggt gtgcctggcg
      961 acgttgccag ccgggcgttg cacgataccc gcctcacccg ccgtcacgtg atgaaaggat
     1021 tactgctgtt gctcggcgct ggcggaggct ggcagctctg gcagtcggaa accggcgaag
     1081 gtctgcgggc agattaccgc accgccaaag gcacagtcag ccgtcagcaa ctggaagatg
     1141 gctccctgct cacgctgaat acccaaagcg cggcggatgt gcgttttgat gcgcatcagc
     1201 gcaccgtccg gctctggtac ggtgaaatcg ccattactac cgcgaaagat gccctgcaac
     1261 gccccttccg tgtcctgacc cgtcagggcc agctcactgc tttagggaca gaatttaccg
     1321 tccgccagca ggataatttc acgcagcttg acgtgcagca gcacgctgtg gaagtgcttc
     1381 tcgccagtgc ccccgcgcaa aaacgcatcg tgaacgctgg tgaaagcctg cagttcagcg
     1441 cctctgagtt tggcgcagtg aaaccgctgg atgacgagag tacaagctgg acgaaggaca
     1501 tcctgagctt cagcgataaa ccgctgggtg aggtgatagc cacgctaacc cgttaccgca
     1561 acggcgtgct gcgctgcgat cccgccgttg ccgggctgcg cctgagcggg acgttcccgc
     1621 tgaaaaatac cgatgcgatc ctgaacgtta tcgcgcaaac gcttcccgtt aaaattcagt
     1681 ctattacgcg gtactggata aacatttcac cactgtaagg aaaataattc ttatttcgat
     1741 tgtccttttt acccttctcg ttcgactcat agctgaacac aacaaaaatg atgatgggga
     1801 aggtatgacg ccgttacgcg tttttcgtaa aacaacacct ttggttaaca ccattcgcct
     1861 gagcctgctg ccgctggccg gtctctcgtt ttccgctttt gctgcacagg ttaatatcgc
     1921 accgggatcg ctcgataaag cgctcaatca gtatgccgca cacagcggat ttaccctctc
     1981 ggttgacgcc agcctgacgc gcggcaagca gagcaacggc ctgcacggcg attacgacgt
     2041 cgagagcggc ctgcaacaac tgctggacgg cagcggactg caggtaaaac cgctgggaaa
     2101 taacagctgg acgctggagc ccgcgcccgc accaaaagaa gatgccctga ccgtggtcgg
     2161 cgactggctg ggtgatgcgc gtgaaaacga cgtatttgaa catgctggcg cgcgtgacgt
     2221 gatccgccgt gaggatttcg ccaaaaccgg cgcaaccacc atgcgtgagg tacttaaccg
     2281 catccctggc gtcagcgcgc cggaaaacaa cggcaccggc agccacgacc tggcgatgaa
     2341 ctttggcatc cggggcctga gcccgcgcct cgccagccgc tcgaccgtcc tgatggacgg
     2401 catccccgtc cccttcgccc cttacggtca gccgcagctt tcactggctc ccgtttcgct
     2461 cggcaacatg gatgccattg acgtggtacg cggtggtggt gcggtgcgtt acggaccgca
     2521 gagcgtgggc ggcgtggtga actttgttac ccgtgccatt ccgcaggact ttggtatcga
     2581 ggcgggcgtg gaaggtcagc tcagcccaac ctcttcacaa aacaacccga aagagacgca
     2641 caacctgatg gtgggcggca cagcggacaa cggttttggc accgcgctgc tctactccgg
     2701 cacgcgcggc agtgactggc gcgagcacag cgccacccgc atcgacgacc tgatgctgaa
     2761 aagcaaatat gcgccggatg aggtgcacac cttcaacagc ctgctgcaat attacgacgg
     2821 tgaagccgac atgcccggtg gcctgtctcg cgcggattac gacgccgatc gctggcaatc
     2881 cacccgcccg tatgaccgct tctggggtcg tcgcaagctg gcgagcctgg gctaccagtt
     2941 ccagccagac agccagcata aattcaacat tcaggggttc tacacccaaa ccctgcgcag
     3001 cggctacctg gagcaaggca aacgcatcac cctctcgccg cgtaactact gggtgcgcgg
     3061 tattgagcca cgctacagcc agatctttat gatcggccct tccgcgcacg aagtgggcgt
     3121 gggctatcgc tatttgaatg aatcaacgca tgaaatgcgt tactacaccg ccaccagcag
     3181 cgggcagttg ccgtccggct caagccctta cgaccgcgat acgcgttccg gcaccgaggc
     3241 gcacgcctgg tatctggatg acaaaatcga catcggcaac tggaccatca cgccgggtat
     3301 gcgtttcgaa catatcgagt cataccagaa caacgccatc acaggcacgc acgaagaagt
     3361 gagctataac gcaccgcttc cggcgttgaa cgtgctctat cacctgactg acagctggaa
     3421 tctttatgca aacactgaag gctcgttcgg caccgtacag tacagccaga ttggcaaggc
     3481 tgtgcaaagc ggcaatgtgg aaccggaaaa agcgcgaacc tgggaactcg gtacccgcta
     3541 cgacgacggc gcgctgacgg cggaaatagg gctgttcctg attaacttta acaatcagta
     3601 cgactccaac cagaccaacg acaccgtcac tgcacgtggc aaaacgcgcc ataccgggct
     3661 ggaaacgcag gcacgttacg atctgggtac gctaacgcca acgcttgata acgtttccat
     3721 ctacgccagc tatgcgtatg tgaacgcgga aatccgcgag aaaggcgaca cctacggcaa
     3781 tctggtacca ttctccccga aacataaagg cacgctgggc gtggactaca agccaggaaa
     3841 ctggacgttc aatctgaaca gcgatttcca gtccagccag tttgcggata acgccaatac
     3901 ggtgaaagag agcgctgacg gcagtaccgg ccgcattccc ggcttcatgc tctggggcgc
     3961 acgcgtggcg tatgactttg gcccgcagat ggcagatctg aacctggcat tcggtgtgaa
     4021 aaacatcttc gaccaggact acttcatccg ctcttatgac gacaacaaca aaggcatcta
     4081 tgcaggccag ccgcgcacgc tgtatatgca ggggtcgttg aagttctgat gtgttaacgc
     4141 ccggcttgcc gggcttttag ctggaatgtg attatgttgg catttatccg ttttcttttt
     4201 gcaggcctgc tgctggtgat cagccacgcc tttgccgcca tggttcagga cgaacacggc
     4261 acgtttacac tcgaaaaaac gccacaacgg attgtggtgc tggaactctc gttcgccgat
     4321 gcgctggccg ccgtggacgt cagcccgatc ggtattgccg acgataacga tgcaaaacgc
     4381 atcctgcccg aagtgcgtgc gcacctgaaa ccgtggcagt ccgtcggaac gcgcgcgcag
     4441 ccgagcctgg aagccattgc cgctctgaaa ccagacctga tcattgccga cagcagtcgc
     4501 catgcggggg tttacatcgc cttgcagcaa atcgcgccgg tactgctgct taagtcccgc
     4561 aacgaaacct acgctgaaaa tttgcaatct gcggctatca tcggcgaaat ggtgggtaaa
     4621 aagcgagaga tgcaggcacg tctggaacaa cataaagaga ggatggcgca gtgggccagc
     4681 cagcttccca aagggacacg cgtggccttt ggcacatcac gcgaacagca attcaacctg
     4741 catactcagg agacctggac cggcagcgtg ctggcctctc tggggctgaa cgttcccgct
     4801 gcgatggcgg gtgcgtccat gccgtccatc ggcctggagc aactgctggc ggtcaatcct
     4861 gcctggctgc tggttgccca ctatcgcgaa gagagcattg ttaaacgctg gcaacaagat
     4921 ccgctctggc agatgttaac cgccgcgcag aagcagcagg ttgcttcggt cgacagtaac
     4981 acctgggcgc ggatgcgcgg tatttttgct gcagagcgta ttgccgctga cacggtaaaa
     5041 atcttccacc atcagccgct taccgttgtg aaatgaccgc gataaaacac ccggtgctgc
     5101 tgtgggggct tcccattgca gcacttatta ttattttctg gctgagtctg ttttgctact
     5161 cggccattcc tgtttccgga gcagatgcaa cccgcgccct gctgcctgga cacacgccaa
     5221 cgctaccaga agcgctggtg caaaaccttc gtttgccacg aagcctggtc gccgttctga
     5281 tcggcgcaag cctggcgctc gcgggcacgc tgctgcaaac cctgacccac aacccaatgg
     5341 cctctccttc actgctcggc attaacagcg gcgcggcgct ggctatggcg cttaccagcg
     5401 cgctaagtcc gacgccgatt gcaggctatt ctctgtcgtt catcgcggca tgcgggggcg
     5461 gcgtgagctg gctgctggtc atgaccgcag gaggcggatt tcgtcatacc catgacagaa
     5521 acaaactgat cctcgcgggt atcgcgctgt cggccttttg tatgggcctt acccgcatca
     5581 ccctgctgct ggccgaagat catgcttacg gcatctttta ctggctggca ggcggagtgt
     5641 cccacgcccg ctggcaggat gtctggcagc tcttgccggt ggtggtcact gcagtccctg
     5701 tcgtgttgct gctggcgaat caactgaacc tgctcaacct cagcgacagc accgcccata
     5761 cgctgggagt gaacctgacg aggctacgtt tggtcatcaa tatgttagtg ctgcttctgg
     5821 ttggtgcgtg cgtcagcgtg gcaggtccgg tggcgtttat cggtctgctg gtgccacatc
     5881 tggcgcgctt ctgggcaggc ttcgatcagc gcaacgtact gccagtgagc atgctgctgg
     5941 gggccacgct gatgctgctg gcagatgtac tcgcacgcgc gctggccttc cccggagatc
     6001 tgcccgcagg cgcagtgctg gcgctgattg gcagcccttg ctttgtctgg cttgtgagga
     6061 ggcgaggatg aaaattgcgc tggttatttt catcaccctt gccctggcgg gctgtgcgct
     6121 gttatcactc catatgggag tgatccccgt gccgtggcgc gcgctgctga ccgactggca
     6181 ggccggacgc gagcattatt atgtattgat ggagtaccga ctgccgcgct tgctgctggc
     6241 actgtttgtc ggtgcagccc tcgccgtggc gggcgtgctg atacagggga ttgtgcgcaa
     6301 ccctctggca tcaccggata ttctcggcgt taaccatgcc gccagcctgg cctctgtggg
     6361 ggctctactt cttatgccgt cactgcccgt gatggtgctg ccgctgctgg cctttgcggg
     6421 cggcatggcg gggttgatat tactgaagat gctggcaaag acccaccagc cgatgaagct
     6481 ggcgctcacc ggcgtggcgc tttctgcatg ctgggccagc ctgacggatt atctgatgct
     6541 ctcgcgcccg caggatgtga acaacgccct gctgtggctg accggcagct tatggggccg
     6601 tgactggagc tttgtgaaga ttgccatccc gctgatgatt ttatttctgc cgctgagcct
     6661 gagtttttgc cgcgatctcg acctccttgc actcggcgat gcgcgcgcca ccacgctcgg
     6721 tgtgtcggtg ccccataccc gattctgggc tttgttacta gctgtcgcca tgacatctac
     6781 cggcgtggcc gcctgcggcc cgattagctt tattggtctc gtggtgccgc atatgatgcg
     6841 tagcatcacc ggtggacgtc accgcagact gctgcctgtt tcggccctga caggtgcgtt
     6901 gctgttggtg gttgccgatc tgctggcgag aattattcat cccccactgg agctcccggt
     6961 tggcgtgctg accgccatta tcggtgcgcc gtggtttgtc tggttgcttg tgagaatgcg
     7021 ataaatgact ttacgaactg aaaatctgac ggtcagttac gggacagaca aggtacttaa
     7081 cgacgtttca ctctcactgc caacggggaa gatcaccgcc ctgatcggtc ctaacggttg
     7141 cgggaaatcg acgctgttaa actgtttttc gcggctttta atgccgcagt ctggcaccgt
     7201 atttctcggc gataatccca taaatatgct ctcatcacgc cagttggccc gcaggctttc
     7261 gctgctgcct cagcaccatt taacgccaga ggggatcaca gtccaggagc tggtttcgta
     7321 tggtcgtaat ccctggctgt cactctgggg gcgtctctcc gctgaagaca atgcacgagt
     7381 taatgtcgcc atgaaccaga cccggatcaa tcatcttgcc gttcgtcggt taaccgagct
     7441 ttccggcggt cagcgccagc gcgcatttct ggcgatggtc ctggcccaga atacgcccgt
     7501 tgtattactt gatgagccaa ccacctatct tgatatcaat caccaggtgg acctgatgcg
     7561 gttgatgggc gaactccgga ctcaggggaa aacggtggtc gctgtgctgc acgaccttaa
     7621 tcaggctagc cggtactgcg atcaactggt ggtaatggca aacggacatg ttatggcgca
     7681 aggcacacca gaagaggtga tgaccccagg attgctgaga acagtattca gcgtggaagc
     7741 ggaaatacac cccgagccgg tatctggcag gccgatgtgc ctaatgaagt agattgcaca
     7801 ggccgtaaga accaaaccac gactgaatga aactggactg gcgccagcaa gcctgttcaa
     7861 actggggctg aacttttccg gactctgaaa gattaccaat actcatcgtc catccgcttg
     7921 ctttaggctg acaggttcat aattaacgca aaccagagct gtacaggctt gggcgcggct
     7981 ttcaaaccag tcgtgatcac ggcaatcaat tttgaactct gcttaacgga catttctgta
     8041 taacccttac ggcaacgaaa aacgcgaagt taaaatttta gaaacccaaa aacgtgacat
     8101 gactaagttt agatttcagg ggggggggag atcaaaaaat ttcgctctgt gccagagcgg
     8161 acattcacgg agctggttca ttaccaatga ggttgggctt ttgaggataa atcaatgatc
     8221 agacgccaac gtaaatcaaa agcaaccctg gaacggaatt gctaatccag tttctgacca
     8281 tcgatttttc taaaaagtgt gcgtttgcta ctacttaggt aggtgcagct ttcttaatca
     8341 ccggcagcca cgttatacag gccagttgat ggatcgattg ttatcaatga tatcttcatg
     8401 agtcggtgtc tcacccagct taccgaagct ggcataaagt gaaggcagac gggcccgtcc
     8461 ttctcccttt ttcgccagag ggaaagcgcg aagcatggtg gcagcctccc agtcacaaca
     8521 ataggatggt gtgcacggct gctgacgcca tgattcagcg atagagccgg aaaatacggg
     8581 gtcaaagccg gtatcattaa ccagagtcat cgtgacctgt actgcggctg gatgatctcc
     8641 tgctactgca actgctaggc gaccgcggct cccttcaggt agtctgttgt ggtcaactaa
     8701 aactggcctc cgcgttagag tttttccagt atcggttttc tgattcgttt ggtggtaacc
     8761 caccattata ttcgtgcggt cttagtgcgc tgtaatatcc aacgatatag tccgttattg
     8821 cgtgagctgc atcgctgaag cttacatacc ccgtcgccgg cacccattcg ttcttcagac
     8881 tcctgaagaa gcgctccatt gggctgttat cccagcagtt tccacgccga ctcatactct
     8941 gcctgatccg gtatcgccac agtaattgcc ggaactgcct gctcgtataa tgactgcctt
     9001 gatcgctgtg gaacatcacc ccgacgggct taccacgggt ttcccatgcc atttccagtg
     9061 ctttcatggt gagcctgctg tccggcgaga acgacatggc ccagcccact ggttttcttg
     9121 cgaacaggtc gggaacaacg gcgaggtacg cccagcgctt acccgtcc
//
LOCUS       DAZFFE010000147         9132 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZFFE010000147 DAZFFE010000000
VERSION     DAZFFE010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.147"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(24..350)
                     /locus_tag="ACHJ69_004315"
     CDS             complement(24..350)
                     /locus_tag="ACHJ69_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO5017168.1"
                     /translation="MKRTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVERAKPGEYVQGING
                     KWIRKF"
     gene            complement(358..543)
                     /locus_tag="ACHJ69_004316"
     CDS             complement(358..543)
                     /locus_tag="ACHJ69_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO5017169.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(540..3179)
                     /locus_tag="ACHJ69_004317"
     CDS             complement(540..3179)
                     /locus_tag="ACHJ69_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO5017170.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLSVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVAAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPALVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVVKPRLILDKPVVWGRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEDKQ"
     gene            3387..4376
                     /gene="ldhA"
                     /locus_tag="ACHJ69_004318"
     CDS             3387..4376
                     /gene="ldhA"
                     /locus_tag="ACHJ69_004318"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO5017171.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDIISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4487..4909
                     /gene="hslJ"
                     /locus_tag="ACHJ69_004319"
     CDS             4487..4909
                     /gene="hslJ"
                     /locus_tag="ACHJ69_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO5017172.1"
                     /translation="MKKVAALVALSLLMAGCVSSDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4906..5172)
                     /locus_tag="ACHJ69_004320"
     CDS             complement(4906..5172)
                     /locus_tag="ACHJ69_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO5017173.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5446..8970
                     /gene="nifJ"
                     /locus_tag="ACHJ69_004321"
     CDS             5446..8970
                     /gene="nifJ"
                     /locus_tag="ACHJ69_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO5017174.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAASVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILELMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTSEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRNVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPVRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSAAEVWSRLPQEVQAVLNQKKACFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNSEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2274 a         2402 c         2243 g         2213 t
ORIGIN      
        1 atcccgatat tgcggttttg ctattaaaat tttcgtatcc atttaccgtt tatcccttgt
       61 acatattccc cgggtttggc ccgttctacg agcttttgcc cggcgagttt cgcgatatca
      121 tccaccgaca cattattttg ctttgccagt tgttgatagc tcgctttacg ttcggcatta
      181 atgtcggtta ctaatttctc agtttcagca tctgttttca gcgcaaccag ataaccataa
      241 aatgtttccc ctacccgccc ctgagttctg gcttcatcca gagtcaatgc cattacattg
      301 ctgcttacca agccaacaag aaatgcacaa agaagtaatg ttcttttcat catcacctca
      361 aaaaagatcg ctacgggtct caagcagctc ttcgacatct ttgtctgcct tgatgatgat
      421 ctcatgctca attttaacgt tcatattgat agtgatcggt tccttaggtg cagcgacttc
      481 aatgcgaggc gtacagccaa ccagcataaa agatgacgtc aacgcagcca gtaaaatttt
      541 cattgtttat cctcacactc ttttccttgc ggacagtcat ttcccggcag acgcgcgttc
      601 tgctccagcc atgcctggag attatcgcca aagcgtaaac tgcgccacag cgtaaaaata
      661 ttctcttcat gatggtaatt aagatttacc gtaccgcttt taccatcaac gcgactggta
      721 cctgtaatat tggcctgcat ggttaataaa ccgagattat ctaaattaat ttttgtcgat
      781 gaacggctaa tttccatata gcgcaaccag ttaattgctg aacccgcggt catattgtct
      841 ttcaccaccg catctgcggt gtctttatcc agccgcaagg tcatcggccc gctatttgcc
      901 agccagccgt ctttcactat ccatttttcg ttgtttagcc ataacggcaa tgcaccacta
      961 aatgccccgg acatggcgaa ttgtttcgga ttgacggcgc taaccagttc gctggaggaa
     1021 agattattca gccgcaacag cgccgggtca tgttgcggca tacgtaattg tttcatcagc
     1081 acgttaccgc ctaacacatc gacgctaaca tcagtcaaca gcaatggttc ttcttcagtc
     1141 cacggataac gaccttgcaa atcagccgta atatttttcg ctgtcaccag attaatcact
     1201 tcggcaattc gcaacgtaac gggaccgcga gtccccagat gccaggctcc atcggcaaaa
     1261 cggaaaggca ggacaaaatc gacaccgtta acctgattat ctggcatcca ggcactaccg
     1321 cctttcaaca cgccgtgccc tcccgcgcgg aatccttgtt cgggcgctgc tgaaaatgcg
     1381 acctgagcat acagttcacc atcgcgtaag ttcatcttcc agtcgggtgg caccaacggc
     1441 tggaatacag tcagtgattg tttaggccac caggcgttac cgcgcagacg aataccgtcc
     1501 cagcgaccat ttacccgaac cgggccaatc tcaccagcat gtaaatcgcc tttaaagagg
     1561 aaatacgtcg gatcgcgccc atcgacacta aattttaagg ttgatggcgg taacacactg
     1621 ccgccagtga acagcgtttg cccggcgtcc agtgacagag cgccgctgaa ggagggatgc
     1681 tgtgcgtcac gtccccagac gacgggcttg tcgagaatta atctcggctt tactaccgtc
     1741 atcgtaccgt attgtaactg gtcgaaaccg gtggagagat cggtcagcgt aatcgtgctg
     1801 tcatgccact caccttttcc tgcgacatcc caggtggcat tcatcggtgt aaaactccct
     1861 tttccccagt agcgccactg ccagcggcca gcgtcaggga gaaaatcatt cgccagccca
     1921 tccatatgca gcacgaaatc gcccagttca ttttcatgcg cctgcaaaat ggcctgcaaa
     1981 cgtccgtcaa caccacgttg ggtgactttt acacccgcta aaggccagcg gatttcatcg
     2041 atgtccagcg aatcaatgac tcttcccttc gaacgtaata acgcgccagg ctcaaaggtc
     2101 agcgttgggt cagacagact tccacttagc tgcgcaggta aacgggcata taaaatgagg
     2161 tccgcctgtt tcgcttcacc ggtcagctgc agaggcagct ggctgttatc catgcttaat
     2221 tttcctgggc caaaatttaa caccgcgtta cctttacccg cttgcccctg ggtcagcaca
     2281 ctcagtcgcc cgctgaccag agcgttctcg agccctgcct gccagttgtc gactttaaca
     2341 cccagtcggc cactcaaagg aaaacctgca tacggccagc tccagcgacc atcgcttacg
     2401 gtcaattgtt gacgagtaat ttgccacggc aaatcgagca acggatcgcc gttatcccgt
     2461 gccagcacaa tcaattgccc gctattttcc tgccagtcca gctcggcatc caccagtgca
     2521 ggctcctgcg gtaagtttaa cgtcgcagta gcatgaccac ttaccggaag tccatccggc
     2581 acgagcggca tagtaaattc ccccaccagt tttaccggcg gctgattttc aaacgcggcg
     2641 acatccagtt cgctgactgt aagttgttgc cctttcagct ggccttgaaa tttaactttt
     2701 tcgccctgat aacgcagttg ctggatatcg gaggttaatg cgagagagag ttttccctgc
     2761 cattcctgcc agggagaaaa aatcagttta tcgatattga tccaggtgtt aggcagcatg
     2821 gactgccact gcgcgagggt ttttggagcg gctggcgatt gctccgtctg cggcaatttc
     2881 gccagacaag cagaatcaag ttctaccgtg ccgacgctca ataaccagcg gctgggatgt
     2941 gaaaggctgg cgttggtgat atgcgcaagc tgacaatctc ccaccagata acggagatcg
     3001 gggatgatta aacctttacg cgtaatgcgt gggctttcat ctaatgcaat acgtgtcccg
     3061 agcggtagcc agatgcccgc cagcgtggga acccacagcc cgagcgtcat cagcagcgtc
     3121 aacggcacaa gaataatcag taataacagc gcgagaacgg ctttatattt acccagcatg
     3181 ggtagttaat atcctgattt agcgaaaaat taagcattca atacgggtat tgtggcatgt
     3241 ttaaccgttc agttgaaggt tgcgcctaca ctaagcatag ttgttgatga atttttcaat
     3301 atcgccatat ctttcaatta tatttgaaat tttgtaaaat atttttagta gcttaaatgt
     3361 gattcaacat cactggagaa agtcttatga aactcgccgt ttatagcaca aaacagtacg
     3421 acaagaagta cctgcaacag gtgaacgagt cctttggctt tgagctggaa ttttttgact
     3481 ttctgctgac ggaaaaaacc gctaaaactg ccaatggctg cgaagcggta tgtattttcg
     3541 taaacgatga cggcagccgc ccggtgctgg aagagctgaa aaagcacggc gttaaatata
     3601 tcgccctgcg ctgtgccggt ttcaataacg tcgaccttga cgcggcaaaa gaactggggc
     3661 tcaaagtagt ccgtgttcca gcctatgatc cagaggccgt tgctgaacac gccatcggta
     3721 tgatgatgac gctgaaccgc cgtattcacc gcgcgtatca gcgtacccgt gacgctaact
     3781 tctctctgga aggtctgacc ggctttacta tgtatggcaa aacggcaggc gttatcggta
     3841 ccggtaaaat cggtgtggcg atgctgcgca ttctgaaagg ttttggtatg cgtctgctgg
     3901 cgttcgatcc gtatccaagt gcggcggcgc tggaactcgg tgtggagtat gtcgatctgc
     3961 caaccctgtt ctctgaatca gacattatct ctctgcactg cccgctgaca ccggaaaact
     4021 accatctgtt gaacgaagcc gccttcgatc agatgaaaaa tggcgtgatg atcgtcaata
     4081 ccagtcgcgg tgcattgatt gattctcagg cggcaattga agcgctgaaa aatcagaaaa
     4141 ttggttcgtt gggtatggac gtgtatgaga acgaacgcga tctgttcttt gaagataaat
     4201 ccaacgacgt gatccaggat gacgtattcc gtcgcttgtc tgcctgccac aacgtgttgt
     4261 ttaccgggca ccaggcattc ctgacagcag aagctctgac cagtatttct cagactacgc
     4321 tgcaaaactt aagcaatctg gaaaaaggcg aaacctgccc gaacgaactg gtttaatctt
     4381 gccgctctcc tgcattccag gggagcttat tcagataatc cccaaagacc tttcatcctc
     4441 tattcttaaa atagtcctga atcagaaaca gtaattgaga accacaatga agaaagtagc
     4501 cgcgcttgtt gcgctaagcc tgctgatggc gggatgtgta agtagtgaca aaattgctgt
     4561 aacgccagaa cagctacagc atcatcgctt tgtgctggaa agcgtaaacg gtaagcccgt
     4621 gaccagcgat aaaaatccgc cagaaatcag ctttggtgaa aagatgatga tttccggcag
     4681 catgtgtaac cgctttagcg gtgaaggcaa actgtctaat ggtgaactga ccgccaaagg
     4741 gctggcaatg acccgtatga tgtgcgctaa cccgcagctt aatgaactcg ataacaccat
     4801 tagcgaaatg ctgaaagaag gtgcacaagt ggatctgacc gcgaaccagt taacgctggc
     4861 gaccgcgaaa cagacattaa cttataagct ggcggattta atgaattaat agctgccaca
     4921 gctcccggcg gcaagtgact gttcgctaca gcgtttgccg ttgggtaatg cacacatccc
     4981 aatcgccgta ccatccagtt gacgggcaac agaaagcgaa ccgccgatca ttgcacaatt
     5041 tgcttctcca ctactggaca tcgacgcttt taaacctggc gctacgtgcg ccgcagtcgc
     5101 ctgctgaaca ggttcactac tacacgccga caacaataaa gcggcacacc ctacccaaaa
     5161 cgctgctcgc atcttttctt cctctgatct tcaagccaaa cgacaccgcc ataaataata
     5221 ggcagcacag agggcggcgt cgagagctgt cctgcgcgtt gccccgcatt tttacttttt
     5281 tatggctatt tttttgccct ctgtttgatc aaaacattca ttacgctgat gtgggggaca
     5341 caaaagcgaa aatgcagaag aaagccattt gctaaaattg aacgattact actgggcgcg
     5401 cagcaatttc gtgcgcccct catttgcgca atgtaagggt gtcatatgat tactattgac
     5461 ggtaatggcg cggttgcttc ggtggcgttt cgcaccagtg aagttatcgc catctaccct
     5521 attaccccca gttccacgat ggcagaacag gctgatgcat gggccggaaa cggcttgaaa
     5581 aacgtttggg gagacacacc acgcgtggtt gaaatgcagt cggaagcggg tgctatcgct
     5641 accgtgcatg gtgctttgca aactggtgcc ctctcaacat cgtttacgtc atcgcagggt
     5701 ttgctgctga tgatcccaac gctgtacaaa ctggcaggcg aactgacgcc gtttgtcctg
     5761 catgttgcgg cacgtaccgt tgccacacat gcactctcta tttttggcga tcattccgac
     5821 gttatggcgg tgcgccaaac gggttgcgcg atgttgtgtg cagcaagcgt ccaggaagcg
     5881 caagactttg ctctcatttc gcacatcgcg acgctgaaaa gccgcgtgcc atttattcat
     5941 ttctttgatg gtttccgcac atctcacgaa atcaataaaa ttgtcccgct ggccgatgac
     6001 acgattcttg aactgatgcc gcaggcagaa attgatgctc atcgcgcccg ggcactcaac
     6061 ccggaacatc cggtgatccg cggtacgtcc gccaatcctg acacttattt ccagtctcgc
     6121 gaagccacca acccatggta caacgcggtc tatgaccatg ttgaacaggc gatgaatgat
     6181 ttcgctgccg cgacaggtcg tcagtatcag ccgtttgagt attacgggca tccgcaagcg
     6241 gaacgggtga ttatcctgat gggctctgcc attggcacca gtgaagaagt ggttgatgaa
     6301 ttgctgaccc gtggcgaaaa agtcggcgta ttgaaagttc gcctgtaccg ccccttctcc
     6361 gccaaacact tgctgcaagc tctgccggga tccgtgcgca acgtggcggt actggacaga
     6421 accaaagaac ccggtgccca ggcagaaccg ctctatctgg atgtgatgac cgcactggca
     6481 gaagccttta ataatggcga gcgcgaaacc ctgccccgtg tcattggtgg gcgctatggt
     6541 ctttcatcca aagaatttgg cccggactgt gtactggcgg tatttgccga gctcaacgcg
     6601 gctaaaccga aagcgcgctt tacggttggt atttacgatg atgtaaccaa tctgtcactg
     6661 ccgttgccgg aaaacaccct accaaactcg gcgaaactgg aagccttgtt ttatggcctt
     6721 ggtagtgatg gcagcgtttc cgcgaccaaa aacaatatca agattatcgg aaattccacg
     6781 ccgtggtacg cacagggcta ttttgtttac gactccaaaa aggcgggcgg cctgacggtt
     6841 tctcaccttc gcgtgagcga acagccggtt cgttccgctt atctcatttc ccaggctgat
     6901 tttgttggct gccaccagtt gcagtttatc gataaatatc agatggctga gcgtttaaaa
     6961 cctggcggca ttttcctgct caacacgccg tacagcgcag ctgaagtgtg gtcgcgcttg
     7021 ccgcaggaag ttcaggccgt gctaaaccag aaaaaagcgt gcttctatgt gattaacgcg
     7081 gcgaaaatcg cccgcgaatg tggcctggcg gcccgtatta ataccgtcat gcagatggct
     7141 tttttccatc tgacgcagat tctgcctggc gatagtgccc tcgcagaatt gcagggtgcg
     7201 attgccaaaa gttacagtag caaaggccag gatctggtgg aacgcaactg gcaggctctg
     7261 gcgctagcac gtgaatccgt agaagaagtt ccactgcagc cagttaatcc gcatagcgcc
     7321 aatcgaccgc cagtggtttc cgatgccgct cctgatttcg tgaaaaccgt aaccgctgcg
     7381 atgctcgccg ggcttggcga cgccctcccc gtctcggcgc tgccgccaga cggcacctgg
     7441 ccgatgggca ccacgcgctg ggaaaaacgc aatatcgccg aagagatccc catctggaaa
     7501 gaggaactct gtacccaatg taaccactgt gtcgccgctt gcccacactc agctattcgc
     7561 gcaaaagtgg taccgcctga agcgatggaa aacgcccctg ccagcctgca ttcgctggat
     7621 gtgaaatcgc gtgatatgcg cgggcagaaa tatgtcttgc aggtggctcc ggaagattgc
     7681 accggttgta acctgtgcgt cgaagtttgc ccggcgaaag accgtcagaa tccagagatt
     7741 aaagccatca atatgatgtc gcgcctggaa catgtcgaag aagagaaaat caattacgat
     7801 ttcttcctca acctgccgga aatcgaccgt agcaaactgg aacgtattga tattcgtaca
     7861 tcgcagctga ttacaccgct gtttgaatat tccggtgctt gctccggttg tggcgagacg
     7921 ccgtatatta aattgctgac tcagctctat ggcgaccgga tgttgatcgc taatgccact
     7981 ggctgttctt caatatacgg cggtaacctg ccttcaacac catataccac cgatgccaat
     8041 ggtcgtggac cggcatgggc gaactcgctg tttgaagata actccgaatt tggccttggt
     8101 ttccgcctga cggtcgatca acaccgtgtc cgcgtgctgc gtctgctcga tcaatttgcc
     8161 gataaaatcc cggcggaatt actgactgcg ttgaaatcag acgccacgcc agaggttcgt
     8221 cgtgaacagg ttgcagcttt acgccagcaa ctcaacgatg tagccgaagc acatgaactg
     8281 ctgcgtgacg cagatgcact ggtggaaaaa tcaatctggc tgattggtgg cgatggctgg
     8341 gcttacgata tcggctttgg cggtctggat catgtattga gtttgacgga aaacgtcaac
     8401 attctggtgc tggatacgca atgctattcc aacaccggtg gtcaggcatc gaaagcgaca
     8461 ccgctgggtg cagtaactaa gtttggcgag catggcaaac gtaaagcgcg taaagatctt
     8521 ggcgtcagta tgatgatgta cggtcatgtt tatgtggcgc agatttctct cggcgcgcag
     8581 ctgaaccaga cggtgaaagc gattcaggaa gcggaagcgt atccggggcc atcgctgatc
     8641 atcgcttata gcccatgtga agagcatggt tacgatctgg cactcagcca cgatcagatg
     8701 cgccaactca ctgctaccgg cttctggccg ttatatcgct tcgatcctcg tcgtgccgat
     8761 gaaggcaaac taccgctggc gctggattca cgcccgccgt cagaagcact ggaagaaacg
     8821 ttacttcacg agcagcgttt ccgtcgtctg aattcgcagc agccagaagt ggcggaacag
     8881 ttatggaaag atgctgcagc tgatttgcaa aaacgctatg acttcctggc acaaatggcc
     8941 ggaaaagcgg aaaaaagcaa caccgattaa atgctctgga taaggatcat ccaattctaa
     9001 aaaaaagccc ggacgactgt tcgggcttgt cttcttatat gttggaaaat cagtagcaat
     9061 gcaatggccc aacagaaaat aaaatctgtg acaataaagg catataaccc gcgcagaata
     9121 acgtatagaa aa
//
LOCUS       DAZFFE010000148         9086 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFFE010000148 DAZFFE010000000
VERSION     DAZFFE010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.148"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2178
                     /locus_tag="ACHJ69_004322"
     CDS             <1..2178
                     /locus_tag="ACHJ69_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO5017175.1"
                     /translation="MNVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMAT
                     FDGHIVEALMKMPEVNAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGER
                     KETGKYTSAVDLARARVNRQHTENSAENISPVTAVIRREYKQTWKTLDDELAYALWPG
                     DIDAGNIDGSIHRWAKNEVINNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPID
                     IHKDPVALNKYITEYLTTKGVFEDEGTNQIATDTLSSPVPETDAVETAIPDNEKTECK
                     VEVEPSVEREGPFYFLFTDKDGEKYGRANKLSGLDKALAAGATEITKEEYFVRKNSTY
                     SGSQQNTGASDTTAQPGPVKVTADEVNNIMQAANISQPDADELLAVSRGEFVAGISDP
                     NDPKWVKGMETRDSVNQNQQETEQNGQKAEQNSPNALQNEPETKQPEPVVQQEPEKIC
                     TACGQTGGGNCPDCGAVMGDATYQETFNEENQVEVQENDPEEMEGAEHPHKENAGSAQ
                     DHASDNETGETADPLIAVNGHHVITSTSRVWYHLMIDLETMGTNTNAPIVVIGAVFFD
                     PQTGEIGPVFYIVISLTDAMNTGAVPDGGTIEWWLKQSSEARAAILTDQVKLKDALSQ
                     FREFINEYSDEKFVQVWGNDATFDNAILRTSYERLDIPCPWRYHNDRDVRTIVELGKT
                     IDFDARTVIPFEGVRHNALDDARHQAKYVTATIQKLIPSQADF"
     gene            2248..2499
                     /locus_tag="ACHJ69_004323"
     CDS             2248..2499
                     /locus_tag="ACHJ69_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO5017176.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            2519..3814
                     /locus_tag="ACHJ69_004324"
     CDS             2519..3814
                     /locus_tag="ACHJ69_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFO5017177.1"
                     /translation="MREAKMKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTTKNRR
                     VAGELRASVCYAIKTGVFDYAKQFPSSHNLEKFGEARQDLTIKELAEKFLALKETEVA
                     KTSLNTYRAVIKNILSIIGEKNLASSINKEKLLAVRKELLTGYQIPKSNYIVTQPGRS
                     AVTVNNYMTNLYAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACR
                     NQQAKNLWCVSVYTGIRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNR
                     VIHLIKPAIDALRSQMTLTRLSKEHIIDVHLREFGRTEKQKCTFVFQPEVSARVKNYG
                     DHFTVDSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQM
                     VFQVYGKWMSENNNAQVALLNTQLSEFAPTMPQNEVVQN"
     gene            complement(4002..5021)
                     /gene="rspB"
                     /locus_tag="ACHJ69_004325"
     CDS             complement(4002..5021)
                     /gene="rspB"
                     /locus_tag="ACHJ69_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO5017178.1"
                     /translation="MKSILIEKPNQLSIIERKIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGDGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GESFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(5033..6247)
                     /gene="rspA"
                     /locus_tag="ACHJ69_004326"
     CDS             complement(5033..6247)
                     /gene="rspA"
                     /locus_tag="ACHJ69_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO5017179.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFDEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGEKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(6453..6779)
                     /gene="ynfA"
                     /locus_tag="ACHJ69_004327"
     CDS             complement(6453..6779)
                     /gene="ynfA"
                     /locus_tag="ACHJ69_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO5017180.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALIWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            6914..7255
                     /gene="ynfB"
                     /locus_tag="ACHJ69_004328"
     CDS             6914..7255
                     /gene="ynfB"
                     /locus_tag="ACHJ69_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO5017181.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QRAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVIIP"
     gene            7290..7850
                     /gene="speG"
                     /locus_tag="ACHJ69_004329"
     CDS             7290..7850
                     /gene="speG"
                     /locus_tag="ACHJ69_004329"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO5017182.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(7853..8563)
                     /gene="ynfC"
                     /locus_tag="ACHJ69_004330"
     CDS             complement(7853..8563)
                     /gene="ynfC"
                     /locus_tag="ACHJ69_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO5017183.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDNHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            8671..8976
                     /gene="ynfD"
                     /locus_tag="ACHJ69_004331"
     CDS             8671..8976
                     /gene="ynfD"
                     /locus_tag="ACHJ69_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO5017184.1"
                     /translation="MKLSTCCAALLFALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDNAPAEPQ"
BASE COUNT         2650 a         2191 c         2066 g         2179 t
ORIGIN      
        1 atgaacgtca cttttgacaa gctggcccct gaagtacaga acgctgtcat ggttaagttc
       61 gacacatgtg aaaacatcac cgttgatatg gtgattagtg cacaggaact gttgcaggaa
      121 gacatggcaa cattcgacgg acatatcgtt gaagcgttga tgaaaatgcc agaagttaac
      181 gccatgtatc cagaacttaa gttgcatgcc atcgggtggg ttaagcataa atgtaagcct
      241 ggagctaaat ggcccgaaat tcaggcagag atgcgcatct ggaaaaaacg tcgcgaaggt
      301 gaacgcaagg aaaccggaaa atacacgtct gctgttgatc tcgcccgcgc cagagtcaac
      361 cgacagcaca ctgaaaactc agcagaaaat atctcccctg tcactgcagt cattcgtcgc
      421 gaatataagc agacatggaa aacactggat gatgaactgg cctacgctct gtggcctggt
      481 gacattgatg ccggaaacat tgacggcagc atccatcgct gggcaaaaaa tgaagttatc
      541 aacaacgacc gcgaagactg gaagcgtatc tcagcatcaa tgcgcaaaca gcctgatgcc
      601 cttcgctacg accgccagac tatttttggc cttgtccgtg agcgtccgat cgacattcac
      661 aaagaccctg tggcactgaa caaatacatt actgaatacc tgactacaaa gggcgtgttt
      721 gaagatgaag gaacaaatca gatcgcaact gatactctct cgtcgccagt accagaaact
      781 gatgcagtgg aaacggcaat tccggacaac gaaaaaaccg aatgcaaagt ggaagtcgaa
      841 ccatctgtag agcgtgaggg gccgttctac ttcctcttca ccgacaagga tggcgaaaaa
      901 tacggtcgcg caaacaaact ttctggtctg gataaggcac tggctgccgg ggctactgaa
      961 atcacgaaag aagaatattt cgtccgcaaa aacagtacat actcaggttc acaacaaaat
     1021 actggtgcat ctgacacgac cgcacaacca gggccggtaa aagttaccgc tgacgaagta
     1081 aacaacatta tgcaggcagc caatatcagc cagcctgacg ccgatgaact gcttgcagta
     1141 tcacgtggtg aatttgttgc aggaattagc gatccgaatg atccgaaatg ggtgaagggg
     1201 atggaaaccc gcgattctgt gaaccaaaac cagcaagaaa cggaacagaa cggccagaaa
     1261 gcggaacaaa acagcccaaa tgcgttacaa aatgagccag aaacgaaaca acctgaacca
     1321 gtagtgcaac aggaaccgga aaagatctgc accgcctgcg gtcagaccgg cggcggcaac
     1381 tgccctgatt gtggcgcggt gatgggcgac gcaacatacc aggaaacatt caatgaagag
     1441 aatcaggttg aagttcagga aaatgatccg gaggaaatgg aaggcgctga acatccacac
     1501 aaggagaatg ctggcagcgc tcaggaccac gccagcgata atgaaactgg cgagacggca
     1561 gatcccttaa ttgcggtgaa cggtcatcac gttatcacat ccaccagcag agtgtggtac
     1621 cacttgatga tcgaccttga aacaatggga accaacacca atgcgcccat cgtggttatt
     1681 ggtgcggttt tcttcgaccc acaaacaggg gaaatcgggc cagtatttta tatcgttatc
     1741 agtctgactg acgcaatgaa tacaggggct gttcctgacg gtggaaccat cgaatggtgg
     1801 ctgaagcagt ccagtgaagc cagagctgcc attttaacag accaggtaaa actgaaggat
     1861 gccctttcgc agtttcggga gttcatcaac gaatactcag atgaaaaatt cgttcaggta
     1921 tggggtaatg atgcgacttt cgataacgca attttgcgca cctcatacga acgtctggac
     1981 atcccctgcc cgtggcgcta ccacaacgat cgcgatgtac gcacaatcgt tgagctggga
     2041 aaaacaatcg actttgatgc cagaaccgtt attccattcg aaggcgtgcg ccacaatgca
     2101 ctggatgacg cccgtcacca ggcaaaatac gttacagcta cgatacaaaa actgatcccg
     2161 agtcaggctg atttttaatg ttcaaccacc gccggttgtg actggtattc tgcaaccggc
     2221 gctcatctga agtaagagat aaaagcgatg agcgaagtaa tcatgattgt ctctcccggt
     2281 aaatgggtat ccgaagaaca gctaattgcg ctgaaaggaa taaaaaaggg aacgctaaaa
     2341 aaagccaggg aaaaatcgtt tatggaagga agggaatata agcatgtagc ccatgacggt
     2401 atgccatggg ataacagccc atgcttttac aacctggaag aaattgatcg ctggattgaa
     2461 cgtcaggcat cagcaagacc aagacgtcat cttacttgac taaaaacaat actaaccaat
     2521 gagagaagct aaaatgaaat atccgacagg cgtggaaaac catggaggga aattacgtat
     2581 ctggtttgtt tataaaggtg taagagtcag ggaaaatctg ggggttcctg acacgacaaa
     2641 aaacaggcgc gttgcaggtg agctgcgcgc ctctgtttgt tatgcaataa aaaccggcgt
     2701 tttcgactat gcaaaacagt ttccctcctc acacaatctg gaaaaatttg gtgaggcccg
     2761 acaagattta accataaagg aactggctga aaaatttctg gcactgaaag aaacggaagt
     2821 cgcaaaaacg tcactcaaca cgtaccgtgc cgtcatcaaa aatattctga gcataatcgg
     2881 tgaaaaaaat cttgcctcgt cgattaataa agaaaaattg ctggcggtac gtaaagaatt
     2941 acttactgga taccagatcc ccaaaagtaa ttatattgtt acgcaaccgg ggagatcggc
     3001 tgtcaccgta aataattaca tgacaaatct ttacgccgtg ttccagttcg gtgttgataa
     3061 cggttacctg gcagataatc cgtttaaggg gatctcgcca ttaaaggaat caagaaccat
     3121 tcctgatcct ctttcacggg aggaatttat ccgtcttatc gacgcctgca gaaatcagca
     3181 ggccaaaaat ttatggtgtg tttctgttta tacaggcatt cgccctggtg aactgtgtgc
     3241 gcttgggtgg gaggatatag atctgaaaaa tggaacaatg atgatcagga gaaatttagc
     3301 aaaagatcgt tttacggtac cgaaaacaca ggctggtacc aatcgggtaa ttcaccttat
     3361 caagcctgca atcgacgctc tcaggagtca gatgacacta acgaggctga gcaaagaaca
     3421 tattattgac gttcacctca gagagttcgg cagaacagaa aaacaaaaat gcacctttgt
     3481 ttttcaacct gaagtgtcag cgagagtaaa aaattatggt gaccatttta ccgttgactc
     3541 aataaggcag atgtgggacg cagcgataaa gcgcgctggc ctccgccacc gcaaatcata
     3601 tcagtcgaga catacttatg cctgctggtc gctgacagcc ggtgccaacc cggcattcat
     3661 agcaaaccag atgggacatg cagatgcgca aatggtgttt caggtatacg ggaaatggat
     3721 gtctgaaaac aataatgcac aggtagccct gttaaataca cagctaagcg aatttgcccc
     3781 aaccatgccc caaaacgagg tggtgcaaaa ttaatttatt aattatcaaa tagttactat
     3841 tcatgactct tgaaatccat aaattcaagc gcagtgccca accatcccga tactgctgct
     3901 ttcaccaaat ccttagtact tctttcgtgt ttttctattg tcataatggt tatctctaaa
     3961 aaagaggtaa gatgcgtact acttactcgc cgttattggt attattcaga aaaagtgagt
     4021 aagactttgc agcaatgttt ttgatcctgt tcaaataaac taatggcatc cgcaacatgc
     4081 tggaaatcaa atgtatgggt aattagtttt tctggtttaa ttaacccttt acttaaccag
     4141 tcgataacga ccgggaattt atttgcattt aagcgtgaag agaaaataga gagttctttt
     4201 ccggtaattc cttgctgaat cacttcagac ggttcactgg agaagcccat caatacaata
     4261 cgtgccgctg gagaagccag cgttacggcc tctttcaaga ttgaaggatg acaagcggca
     4321 tcgataatta aagtcggctt gatgcctttt tcagcgaaac tctcgccaag cggtgtctgg
     4381 ctgttattaa tcgcccagtc tgccccgctc tctttcgctt tttccagtcg ttcatcaatg
     4441 cgatcggcaa caatcacatt tttaacgtta tagacgcctt ttaatacctg aacgatcgtc
     4501 aggccgattg ggccggcacc gtaaaccaga acggtatcat tttcagtcgg ttgaccatga
     4561 ccggtaacgt tagccgcaat ggtaaaaggt tcgatcatca ccgcatattg atcggccact
     4621 gcttcaggaa ttttccacgc attttttgcc ggaaccacgg catattcact gaaaccaccg
     4681 tcagcgtgca cacctaatac agcaagtgtc gtacaaacgt tcggcttacc tatagagcac
     4741 ggatagcaat gcccacagct gaccaccgga tcgacagcaa cgcgttcacc gactctggcg
     4801 ctttccacgc cgtcacccac tgcatcaatg acgccaaaga attcatgacc aatgacgcgc
     4861 ggatatttcg caaaaggatt atgcccacgg taaatatggc tatctgaacc acaaattccg
     4921 gcaagtttaa cttttactcg tacttcaccc gctgacgggg tgggtatttt acgttcgata
     4981 atcgacagtt gattcggttt ttcaattaaa atgcttttca ttaccttact ccttaccagt
     5041 tccacagcgt gccatcttcc agacgtgcga ctggcagata agcaggttca tagggatatt
     5101 tcgccgccag cttttcatcg aattcgatgc caagacccgg tttttctccc ggatgcatat
     5161 agccgttatc gaaagtccag ttgtgcggga agacttcgag catttgttcg gaataaccca
     5221 tgtattcctg gacaccgaaa ttgggtaccc acagatcaaa gtgcagcgcc gcagccatgc
     5281 agactggtga caaatcggaa ggaccgtgtg agccagtacg tacctgatac agcgaagcaa
     5341 aatcggcaat ccggcgcata ccggtaattc cgcctgcatg ggtcagcgtg gtacggatat
     5401 aatcgatgag ttgctcttca atcagttgtt tgcagtccca aatgctgttg aagacttcac
     5461 ccaccgcgat gggtgtgacg gtatgttggc gaatgagacg gaagcattcc tggttttccg
     5521 caggcgtcgg gtcttccatc cagaacatgc gataatcttc aatgctctta ccaaagcgcg
     5581 ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag cagatgttca tcaaaaccaa
     5641 acttgttacg taccgcgtca aacaatttcg gcatgaaatc gaggtatttc tccgtcgacc
     5701 acagctgctc ttccggccac tgtcctttgg ttgcgggttc ataagccaga cctttacctt
     5761 tcgacatgcc gtaggtggtt ttcataccag ggattccgca ctgcacgcgg atggctttga
     5821 atcccagctc ctgatgacgg gcataatcat ccagagcttc atcaatactg tgaccggtgg
     5881 tatggcaata aaccatcacc ccttcacgag acgcgccgcc gagtaactgg taaagcggca
     5941 tgttggcagc tttggcttta atatcccaca gcgccatatc aaccgctgaa atggccgaca
     6001 tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa aaactgccag atatcttcga
     6061 tacggtgtgc atcgcgacca ataagctgcg gacaaaggtg atcctgcaaa taagaggcca
     6121 cggaaagctc acgtccattt agggtggcat ccccaaggcc cgtaataccg tcctcagtgg
     6181 tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac aaaaacttca gcctttacga
     6241 tcttcatgtt cgattccttg catcgcttgt cgtgatgcat gaaatctacg caactgagct
     6301 actaccatac aagcatgaag atcaaaaaaa gccggagtga tcacaaaaaa aggcgtatat
     6361 ttgcgccgtg aatggttgac aaaagatgaa acagaatacc ttttgtcagc tgacacttcc
     6421 tcgtatctta ttgataaaat ggatttatgt tcctacgtgc gcccccagcc cgcaacaatg
     6481 atcaacatgc cgcaaagcgc aattaacgcc cccgtccagt cataaagagt cagtttcacg
     6541 ccatccacaa cgcgcagcca aatcaacgcc gtgcagacat aaacgccacc ataagccgcg
     6601 taaacacgcc cactcgccgc aggatgcaac gttaacaacc agacaaacag cgccagtgaa
     6661 atccccgccg gaagcaacag ccagatactg gcgtttcgct tcaaccacag ccagggcaaa
     6721 aagcatccaa taatttcaca cagcgcagta gcaaaaaata gtaacgttgt tttaatcatc
     6781 tttgtcactt attgacatca cgtatagtta tagggcgaca taatatcatc aatataaaca
     6841 ccctccttga acgttttgcg ttcgcagtgg atggtgtaga atcagcttta atcattcata
     6901 cagggaatga attatgaaaa tcactctcag caaacgaatc ggcctgctcg cttttctgct
     6961 gccttgcgca ctggcattga gcacaactgt tcatgccgaa actaacaaac tggtgattga
     7021 gtctggcgac agtgcacaaa gccgccagcg cgccgctatg gaaaaagagc aatggaatga
     7081 cacgcgtaat ctgcgccaga aagtgaataa acgcactgaa aaagagtggg ataaagccga
     7141 cgctgctttt gataaccgcg ataaatgtga gcaaagcgcc aacatcaatg cctactggga
     7201 gccaaatact ttgcgctgcc tggaccgtcg aactggccgc gttattatcc cctaacctgt
     7261 tattgattta aggaatgtaa ggacacgtta tgccaagcgc ccacagtgtt aagctacgcc
     7321 cgctggagcg tgaagattta cgctatgtac atcaactcga caataacgcc agtgtgatgc
     7381 gttactggtt tgaggaaccc tacgaagcct ttgttgaact ctctgatctg tatgataagc
     7441 atattcacga tcagagcgaa cggcgctttg tggtggaatg tgacggcgaa aaagccggtc
     7501 tggtggagct ggtggaaatt aaccatgttc atcgccgcgc agaatttcag ataattatct
     7561 ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc caaattagca atggactatg
     7621 gcttcaccgt tctcaatctc tataagctgt atctgatcgt tgataaagag aatgaaaaag
     7681 cgattcacat ttaccgcaag cttggcttta cggttgaagg tgaattgatg catgagttct
     7741 ttattaatgg tcaatatcgt aatgccattc gcatgtgtat attccagcat cagtatctgg
     7801 ctgagcacaa aacaccgggt cagactctcc tgaagccgac cgcacaatag tattaataat
     7861 aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc gggttttact ccttcatcaa
     7921 caatgattag ctgacagtcc accggattgg cgtgattgtc atattcacag ctctgtttta
     7981 catttccaac ccgttgatta ttcagtaaag taaccgctgt gtaatctaat tttttgatcg
     8041 ggtccgttga tggcgtggcg ctgacagata atgttttatc gttacttttc gtggttttac
     8101 ccagcggata cccctgatca tcatagcgat attccatctg catctgtttg ctgctggctt
     8161 taatcacgaa accattatca tcggtttccc aactcacccc ggcagaaggt aattctgcta
     8221 gctggcattt tccctgcaaa cgaactcttt tctccagcgt ctcggcatca cggtaataat
     8281 tggcgtccag taccagggca accacggtat tattttccag atccagtaat tcgagagaat
     8341 caaaacagcc ttcttccgac aaagtccccg aaacacgttt cgtcacttca ccttgctcat
     8401 ccattaatgt ctgagtgaaa tcttttacgg gaccacgcag cggatcaaaa tcgaattcat
     8461 tagagaaact ggccatctcc ggggtaaatg aaagtgttac ctctgtgcgg tcacatcctg
     8521 tgagtaatat cgcgagtaag catggcagta atttgtattt cacaacagtc acctgagagt
     8581 agagatgatt ctcaatcata gtagcaaata cagtacttta cacgttaaat gctatgctta
     8641 aagaagttat cttcgcgtaa ggagcttatg atgaaactct caacctgctg tgccgcgctt
     8701 ctgttcgccc tcgcctcacc cgcggtactc gccgcgccgg gatcctgtga gcgcatacag
     8761 agcgatatat cacagcgcat tatcaataat ggcgtacccg aaagcagctt cacgttaagt
     8821 attgtaccca atgaccaggt tgatcagcct gattcccagg tcgtcgggca ttgcgctaat
     8881 gatacgcata aaattctcta cacccgtacc accagcggta acgtctctgc tcccgcgcag
     8941 tctacccagg ataacgcgcc tgctgaaccg cagtaacaca ttatcgactg aacgccggat
     9001 acgacacatc cggcgatttg aacatacaac ataatcccac cttattactc atacccttct
     9061 attgatatgg attaataatt attaac
//
LOCUS       DAZFFE010000149         9077 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZFFE010000149 DAZFFE010000000
VERSION     DAZFFE010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.149"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(113..937)
                     /locus_tag="ACHJ69_004332"
     CDS             complement(113..937)
                     /locus_tag="ACHJ69_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO5017185.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            1257..3287
                     /gene="malS"
                     /locus_tag="ACHJ69_004333"
     CDS             1257..3287
                     /gene="malS"
                     /locus_tag="ACHJ69_004333"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO5017186.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLMLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFAEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPNNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSKADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTSASGLPVFYKNKTDTHAKDIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYCYGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            3465..4718
                     /gene="avtA"
                     /locus_tag="ACHJ69_004334"
     CDS             3465..4718
                     /gene="avtA"
                     /locus_tag="ACHJ69_004334"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO5017187.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(4870..5343)
                     /locus_tag="ACHJ69_004335"
     CDS             complement(4870..5343)
                     /locus_tag="ACHJ69_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO5017188.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHTYTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(5445..6293)
                     /gene="yiaJ"
                     /locus_tag="ACHJ69_004336"
     CDS             complement(5445..6293)
                     /gene="yiaJ"
                     /locus_tag="ACHJ69_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFO5017189.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            6494..7492
                     /gene="yiaK"
                     /locus_tag="ACHJ69_004337"
     CDS             6494..7492
                     /gene="yiaK"
                     /locus_tag="ACHJ69_004337"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFO5017190.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTEDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTSAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            7504..7968
                     /locus_tag="ACHJ69_004338"
     CDS             7504..7968
                     /locus_tag="ACHJ69_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO5017191.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRTTDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPACNKNATTAIRKIVVKVALAAL"
     gene            7991..8911
                     /locus_tag="ACHJ69_004339"
     CDS             7991..8911
                     /locus_tag="ACHJ69_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4862 family protein"
                     /protein_id="HFO5017192.1"
                     /translation="MKTNNAGYIIGAYPCAPSFHQRSEEEEKDFWRQLSETPDILGLE
                     QPCLENFHPLGDQWLLRHTPESWKFVVTAVMETMRRRSENSGFGLASSDEEQRQACVA
                     YYRHLFNKINSLQANKVLALELQAAPLATNPNVMQATDAFARSLKEIASWDWPCKLVL
                     EHCDAMTSSSPRKGFLPLENVLEVITDYDISICINWARSAIEGRNTTLPLTHTQMAKQ
                     AGKLGALMFSGTTLNGAYGEWQDLHAPFAPFCAESLMTTDHVRELFNVAESSTLHFAG
                     IKLLEINATADVHHRIEILRNGIHSLNESR"
BASE COUNT         2313 a         2307 c         2236 g         2221 t
ORIGIN      
        1 tgtagcgatg atgagaatta tcggcgtcgc atcaggtaac tcagcacaac cacctgatac
       61 gagcaaaagc cttatccgcc tgacaagtcg gataaggctc gaggaaatgc aattacatat
      121 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
      181 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
      241 ccgctttacg cagttgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattga
      301 ggttagtgac ataggcgctc accgattctt taacagaact aaactgtgag taccctttca
      361 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac aggttgttgt
      421 tgttgcgcgc cagctttgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
      481 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
      541 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
      601 gtgaccattg gccctgatac tgttttgaaa tcagccagtt acgttccgca gtaatggccg
      661 cattttggct ggtaatgtaa ggcattacag tccggagaaa cgcctttttc cttggtgttc
      721 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
      781 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
      841 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
      901 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
      961 cattccaaca ccttatattt tttacaaatt tgagagttga atctcaaatc atatcaaaaa
     1021 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
     1081 ttcagattgg cgctaaatcg cagaaaatgt ggggtttatc gcaaaattca gccgtttttt
     1141 gcgcgcgatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
     1201 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccgccatga
     1261 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
     1321 ctccggggtt ccctgccttt agcgaacagg ggacgggaac atttgtcagc cacgcgcagt
     1381 tgcccaaagg tacgcgtcca ctaatgctaa attttgacca acagtgctgg cagcctgcgg
     1441 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
     1501 ggcgattgtt cagggacggc gaatatacgc tgcaactaga cacccgctcc ggtacgccaa
     1561 cattgatgat ttccatccag aacgccgtcg aaccggtagc aagcctggtc cgtgaatgcc
     1621 cgaaatggga tggattaccg ctcacactgg atgtcagcgc cacttttgcg gagggagccg
     1681 ctgtccggga ttattacagc cagcaaattg cgatagtgaa gaacggtcaa ataacgttac
     1741 aacccgctgc caccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg
     1801 cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg
     1861 gcgatcccaa taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca
     1921 cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattatctc cagcagttgg
     1981 gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggcg
     2041 gtacaaaagg tgatttcccg cattatgcct atcatggtta ttacacacag gactggacga
     2101 atcttgatgc caatatgggc agtaaagccg atctacggac gctggttgat agcgcgcatc
     2161 agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccggctat gccacgctgg
     2221 cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgag ctgaaaaaaa
     2281 cgctgggtga acgctggagc gactggaaac ctgccgccgg gcaaacctgg catagcttta
     2341 acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga
     2401 tcagaacgga tatcggcgat tacgacaatc ctggattcga cgatctcact atgtcgctgg
     2461 cctttttgcc ggatatcaaa accgaatcaa ctagcgcttc tggtctgcca gtgttctata
     2521 aaaacaaaac ggatacccac gctaaagaca tcgacggctt tacccctcgc gattacttaa
     2581 cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg
     2641 ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc gctgcacttc
     2701 gcgaatggaa aaaagccaac cccgacaaag cattagatga caaacctttc tggatgaccg
     2761 gtgaagcctg gggccacggc gtgatgcaaa gtgactacta ttgctacggc ttcgatgcga
     2821 tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg
     2881 atacgacctg gcagcaaatg gcggagaaat tgcaggattt caacgtgttg agctacctct
     2941 cgtcgcatga tacccgcctg ttccgtgaag ggggcgacaa agcagcagag ttattattat
     3001 tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta
     3061 ccggttctga tccgctgcaa ggtacgcgtt cggatatgaa ctggcaggat gttagcggta
     3121 aatctgccgc cagcgtcgcg cactggcaaa aaatcagcca gttccgcgcc cgccatcccg
     3181 caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg
     3241 agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc
     3301 ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcat
     3361 tgcaggataa agcaccgctc actcttcaac agagcgattt gcaccccagc aaatgtagcg
     3421 ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt
     3481 ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg
     3541 gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa
     3601 tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg
     3661 cactgtgcaa ttatgacggt ccacagggga aaacggagct actcacactg cttgccggaa
     3721 tgctgcgcga gaagttgggc tgggatatcg aaccacagaa tattgcacta acaaacggca
     3781 gccagagcgc gtttttctac ttatttaatc tgtttgccgg acgccgtgcc gatggtcggg
     3841 tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg
     3901 aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta
     3961 aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct
     4021 cccggccgac gaatccaaca ggcaatgtga ttactgacga agagttgctg aagcttgacg
     4081 cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc
     4141 cgggtatcat cttcagcgaa gcgcgcccgc tatggaatcc gaatatcgtg ctgtgcatga
     4201 gtctttccaa gctggggcta cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa
     4261 tcatcaccgc catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc
     4321 cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca
     4381 tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac
     4441 cggaagatcg ctgcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta
     4501 aggatttgcc cattacgacc gagcagctct accagcgcct gaaagcacgc ggcgtgctga
     4561 tggtgccggg gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat
     4621 gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggtgtg aagattctgg
     4681 cggaagagat agaaagagcc tgggctgaaa gccactaaaa attgcctgat gcgctacgct
     4741 tatcaggcct acatgatttc tgcaatatat tgaatttggt agaatttgta ggccggataa
     4801 ggcgttttag ccgcatccgg cataaacaaa gcgcactatc cctgcgttga gaaacgccgg
     4861 aaaaacgttt caaacaggct gctgccgtag ccgatgccgc tgcactttct cgacatccat
     4921 gcactgcaac gcctttgtcg ggcaggcttc aacacaggcc ggccccgtcg cccgatgcca
     4981 gcacagatcg cacttaatcg ccctcgcctt tttgcgcgaa gaaacgacct ccatcgcacc
     5041 aaacgggcaa gccagcatac agcttttaca gccaatgcaa cgtgattgtt caacgaaaat
     5101 atgcccatgt tcgcggctta tcgcgtcaac agggcacaca tttgcgcacg gtgcatcttc
     5161 acactgatga caggctactg ccgtggtgta agtatgatct ttaatgacac gaatacggga
     5221 aataaactcg tctggtgaca acgcagcgca atcctgattc tcctgatgcg acactgcgca
     5281 agccacttca caggtacggc aaccgataca tttcgtcgca tccgcaataa taaaccggtt
     5341 catgatcact cctttgcact aaaacagcag agtgccagag agcgcgtccc tactgctttg
     5401 atccaacgaa ggaaatgtta aagttttggc acttgtccaa agggttatgt aatcgcgccc
     5461 tggtcgtccc tgacagtaaa tcccagttca ttagaaatag cctgcgcggt ttcacgaagt
     5521 ggtttcagga gatttttctc tcccacctgt ttcagacgtg atgtcgaaag cgaaatcgac
     5581 acggcgtacg gcacccgccc atgaatatca aacaccggaa cagcaataca ggagacgccg
     5641 agttcgtttt cttctctgtc catcgccgct ccactttcac gaatgtgcgc cagttcgtcg
     5701 aacatcgcgg gcagctcggt aatggtattg cgggttaacg gctggatctc atgctgatgg
     5761 ctttcccagt atgacttcac gtagtccggg tgaccaaacg ccatgtagat cttgcccatt
     5821 gcggaacagt agagcggcat atgctggcct atataggcac gggttcgcag catcccggtt
     5881 gtgggttcca gcttataaat caaaatagcg tgatcgtctt cgcggctgga gaagttaata
     5941 gtttcaccag tggcgatgtt cagagcctca agatgcggag cggcgatatg aatgatattc
     6001 agcgaagata gcgccttctg cccgacggca ataaatttgg tggtcaggcg ataactccct
     6061 gcggcgggcg cggtggtcac atagccgcag gactgtaatc cctgcaataa gcgatggacg
     6121 gtactcttat ttaaaccagc cagctccgaa agatgcgcca acggacaacc gtttggatag
     6181 ttgctcaaaa tctcaatcag catcaaccca cgaaacagac tctggcttcc ggctgggcgc
     6241 tctttttcct gcgccatctc gttctctttt tttcccatca cttctttccc cattttgtcg
     6301 cgtcctgatg gtagcgcaaa gtgtgccgta gttcacgatc tcgacagata atttataacc
     6361 acttgatttt taagtttttt gaaattcatc aatcagattg ctttgttaaa aagtgatcga
     6421 tatatttgaa atcaagtttc gtatattgaa attttaagcc aaaaaagcga tcaaaaaaac
     6481 aaggaagcct gggatgaaag tgacatttga gcagttaaaa gcagccttta atcgggtctt
     6541 aatttcacgc ggcgttgaca gcgaaacggc tgacgcctgt gcagagatgt tcgcccgcac
     6601 caccgaatca ggcgtttatt ctcacggcgt taatcgtttc cctcgtttca ttcaacaact
     6661 ggaaaacggc gatatcattc ctgatgccca acccaaacgt ataaccagcc tcggcgcaat
     6721 tgaacagtgg gacgcccagc gttcgatcgg taacctgaca gcgaaaaaga tgatggatcg
     6781 cgccattgaa ctggctgccg atcacggtat tggtctggtg gcactacgta atgccaacca
     6841 ctggatgcgc ggcggcagct acggctggca ggcggcggaa aaaggctata ttggcatttg
     6901 ctggaccaac tccatcgccg taatgccacc gtggggcgca aaagagtgtc gcatcggcac
     6961 caacccgctg atcgtcgcca ttccttccac gccgatcacc atggtcgata tgtcgatgtc
     7021 gatgttctct tacggcatgt tagaagttaa tcgcctggct ggtcgtcagc tcccggtaga
     7081 tggtggcttt gatgatgagg gcaatttgac caaagaacct ggcgttatcg agaaaaatcg
     7141 ccgcattttg ccgatgggct actggaaagg ttcgggcatg tcgattgtgc tggatatgat
     7201 cgctaccctc ctttctgacg gcgcatcggt tgccgaagtc accgaggaca acagcgacga
     7261 atacggcatt tcacaaattt ttattgccat tgaagtggac aagcttatcg acggtcccac
     7321 ccgcgatgcc aaactgcaac gcattatgga ttacgttacc agtgccgagc gtgctgacga
     7381 aaaccaggct attcgtttac ccggccacga atttactacc ctgctggcag aaaaccgccg
     7441 taacggtatt accgttgatg acagcgtgtg ggcaaaaatc caggcgttat aaggagataa
     7501 gccatgattt ttggacatat cgcgcagcct aatccgtgcc gtttgcccgc cgccattgaa
     7561 aaggcgctcg attttctgcg caccaccgac ttcaacgccc tggaaccggg cgttgtcgaa
     7621 atcgacggca agaatattta tgcgcaaatt atcgatttaa ccacacgcga ggcggtagaa
     7681 aaccgtccgg aagtgcatcg tcggtatatc gatattcagt ttctggcgtg gggtgaagag
     7741 aaaatcggca ttgctattga tacgggaaat aataaagtca gcgaatcact attagagcag
     7801 cgcgatatta ttttttatca cgatagtgaa catgaatcat ttatagaaat gattccgggc
     7861 agctacgcta ttttcttccc gcaggatgtt catcgcccgg catgtaataa aaatgccact
     7921 acggcaatac gcaagatcgt ggttaaagtg gccctcgctg ctttataaaa aatgattact
     7981 ggagcacata atgaaaacga ataatgccgg ttatattatc ggcgcgtatc cctgtgcacc
     8041 ttcttttcac caaagaagtg aagaggaaga gaaggatttc tggcgacaat tatctgagac
     8101 tccagacatt ttggggctgg aacaaccttg cctggaaaat tttcaccctc ttggcgatca
     8161 atggttactc cgtcatacgc cagagagctg gaaatttgtc gtcaccgcag tgatggaaac
     8221 catgcgtcgc cgcagcgaaa acagcgggtt tggtctcgcc tccagtgatg aagaacagcg
     8281 ccaggcctgc gtggcgtact atcgccacct gttcaataaa atcaactcat tgcaggctaa
     8341 taaagtgctc gcactggagc tccaggctgc accgttagca acgaatccta atgttatgca
     8401 agcgacggac gcttttgccc gctcattgaa agaaatcgcc agttgggact ggccgtgcaa
     8461 gctggtgctg gaacactgcg acgccatgac atcttcttcc ccccgaaaag gctttttgcc
     8521 tttagaaaat gtgctggaag ttattaccga ttatgacatc agcatctgta tcaactgggc
     8581 gcgctctgcc attgaagggc gcaatacaac gctgccgctg acccacaccc aaatggcgaa
     8641 acaagccgga aaactcggcg cgttaatgtt ctctggtacg acgctaaacg gggcatacgg
     8701 cgaatggcag gatttacacg caccgtttgc accattctgc gcagaaagcc tgatgaccac
     8761 agatcatgtt cgcgagttat ttaatgtagc agaatcgtca acattgcatt ttgctggcat
     8821 caagctactg gaaataaatg cgacggctga tgtgcaccat cgcatcgaaa tattacgcaa
     8881 cggcattcac tctctgaatg aatctcgtta aacagaacca tttatatatc actttgaata
     8941 tctaattagc cgttttctga taattattac gaatattaac aacattagcg cataaaaaat
     9001 attgcataac cagaaggact cgttatgaaa cggatactcg aaggcattct tgcgatcatc
     9061 atcgcaatac tgtcttg
//
LOCUS       DAZFFE010000150         8940 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFFE010000150 DAZFFE010000000
VERSION     DAZFFE010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.150"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..387
                     /locus_tag="ACHJ69_004340"
     CDS             <1..387
                     /locus_tag="ACHJ69_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HFO5017193.1"
                     /translation="IFIKPHPKEPKKNINAIQKAIKKAKCRDIILITEPDFLIEPVIK
                     KAKIKHLIGLTSSSLVYAPLVSKRCQSYSIAPLMIKLCDNDKSQKGINTLRLHFDILK
                     NFDNVKILSDDITSPSLHDKRIFLGE"
     gene            complement(537..1742)
                     /locus_tag="ACHJ69_004341"
     CDS             complement(537..1742)
                     /locus_tag="ACHJ69_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFO5017194.1"
                     /translation="MQGNALTVLLSGKKYLLLQGPMGPFFNDVAEWLESLGRNAVNVV
                     FNGGDRFYCRHRQYLAYYQTPKEFPGWLRDLHRQYDFDTILCFGDCRPLHKEAKRWAK
                     SKGIRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPTPHVENLTPSTM
                     KRIGHAMWYYLMGWHYRHEFPRYRHHKSFSPWYEARCWVRAYWRKQLYKVTQRKVLPR
                     LMNELDQRYYLAVLQVYNDSQIRNHSNYNDMRDYINEVMYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKEYGLGERVIYVHDLPMPELLRHAKAVVTINSTAGISALIHN
                     KPLKVMGNALYDIKGLTYQGHLHQFWQADFKPDMKLFKKFRGYLLVKTQVNWVYYGGN
                     TTNCQHNIY"
     gene            complement(1777..3804)
                     /locus_tag="ACHJ69_004342"
     CDS             complement(1777..3804)
                     /locus_tag="ACHJ69_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HFO5017195.1"
                     /translation="MIGIYSPGIWRIPHLEKFLAQPCQKLSLLRPVPQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLGVNGEPPLSLVVDDCGIYYDASKPSAL
                     EKLVQDKAGNAALADQAREAMHTIVTGDLSKYNLAPAFVADESERSDIVLVVDQTFND
                     MSVTYGNAGPHEFAAMLEAAMAENPQAEIWVKVHPDVLEGKKTGYFADLRATQRVRLI
                     AENVSPQSLLRHVSRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSATLEELFAAAYLRYCRYIDPQTGEVSNLFTVLQWLQLQRRHLQQRNGYLWVP
                     GLTLWKSAILKPFLQTATNRLSFSRRCTAASACVVWGVKGEQQWRAEAQRKSLPLWRM
                     EDGFLRSSGLGSDLLPPLSLVLDKRGIYYDATRPSDLEVLLNHNQLTLAQKMRAEKLR
                     QRLVESKLSKYNLGADFSLPAEAKDKKVILVPGQVEDDASIKTGTVSIKSNLELLRTV
                     RERNPHAYIIYKPHPDVLVGNRKGDIPAEQIAELADYQALDADIIQCIQRADEVHTMT
                     SLSGFEALLHGKHVHCYGLPFYAGWGLTVDEHRCPRRERKLTLADLLYQTLIVYPTYI
                     HPTRLQPITVEEAAEYLIKTPRKPMFITRKKAGRVIRYYRKLIMFCKVRFG"
     gene            complement(3801..4541)
                     /gene="kdsB"
                     /locus_tag="ACHJ69_004343"
     CDS             complement(3801..4541)
                     /gene="kdsB"
                     /locus_tag="ACHJ69_004343"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO5017196.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDQRVEKAVQAFGGKAIMTRNDHESGTDRLVEVMHKVEADIYINLQGDEPM
                     IRPRDVETLLQGMRDDPALPVATLCHAISAEEATEPSTVKVVVNTHQDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMQEQAESLEQLRLMSAGINIRTFEV
                     AATGPGVDTPACLEKVRALMAQELAENA"
     gene            complement(4551..6227)
                     /locus_tag="ACHJ69_004344"
     CDS             complement(4551..6227)
                     /locus_tag="ACHJ69_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HFO5017197.1"
                     /translation="MKLFKSILLIAACHAAQASAAIDINADPNLTGAAPLTGILNGQQ
                     SDTQNMSGFDNTPPPSPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQVDPKGNIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            complement(6251..7399)
                     /locus_tag="ACHJ69_004345"
     CDS             complement(6251..7399)
                     /locus_tag="ACHJ69_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFO5017198.1"
                     /translation="MLIKVKSAVSWMRARLSAISLADIQKHLAKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEQSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            complement(7471..8454)
                     /locus_tag="ACHJ69_004346"
     CDS             complement(7471..8454)
                     /locus_tag="ACHJ69_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFO5017199.1"
                     /translation="MSERHLPDDQSSTIDPYLITSVRQTLAEQGAALQNLSKQLDSGQ
                     YQRVLNLIMNCKGHVILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMIT
                     PYDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANET
                     CPNNLAPTTSTTLTMAIGDALAIAMIRQRKFMPNDFARYHPGGSLGRRLLTRVADVMQ
                     HDVPAVQLDASFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSA
                     TAAQMMTREPLTLPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
BASE COUNT         2286 a         2222 c         2066 g         2366 t
ORIGIN      
        1 atatttataa aacctcaccc taaagagcct aaaaaaaata ttaatgcaat tcaaaaggca
       61 ataaaaaagg caaaatgtcg tgacataatt cttataacag agccagactt tttaatagag
      121 ccggtaataa aaaaagcaaa aataaaacac ttaattggat taacatcatc ttctttggta
      181 tatgcacctt tagtttctaa aagatgtcag tcttattcaa tagcgcctct tatgataaag
      241 ttgtgtgata atgataaatc ccaaaaaggg attaatacgc tgcgtctcca tttcgatatt
      301 ttaaagaatt ttgataatgt taaaatatta tcggatgata taacatctcc ctctttgcac
      361 gataaaagga ttttcttggg ggagtaataa aagctacatt ttatattcta ttacagtacc
      421 agtcgtaaaa ttaaatctta aagtagtatg ttttattacc tttatgttac ttttctttga
      481 agaggatgga aatgattttt tggctactta aaattcaaaa gatattgact tgaaatttaa
      541 tatatattat gttggcagtt tgttgtgttc cccccataat aaacccaatt aacctgcgtc
      601 ttcaccaata aatacccacg aaacttctta aacagtttca tatctggttt aaaatcagcc
      661 tgccagaact ggtgcaaatg cccttgatac gtcaagcctt tgatgtcgta cagggcattg
      721 cccatcactt tgagtggttt gttatgaatc agtgcagaga tccccgccgt actgttaatc
      781 gtcaccaccg cttttgcgtg gcgtaataat tccggcatcg ggagatcgtg cacataaatg
      841 actcgctcac ctaagccata ttccttactt agccgcttaa ttaatggtcg atagagtctg
      901 tgaccacgat ccatcggatg atgtttgatc accaaataac tttctttcgg cgctttacgc
      961 gaaaatgagt acatgacttc attaatatag tcacgcatat cgttataatt gctgtggtta
     1021 cgaatctggc tatcgttata tacctgcaaa acggcaagat aataacgctg gtccaactcg
     1081 ttcattaacc tcggtaatac cttacgctgt gttaccttgt aaagttgctt gcgccagtat
     1141 gcacgaaccc agcaacgagc ctcataccag ggggaaaacg atttatggtg gcggtagcga
     1201 gggaactcat ggcggtaatg ccagcccatc aggtaatacc acatcgcatg acctatacgt
     1261 ttcatcgttg aaggtgttaa gttctcaacg tgcggcgtag gcatatctgg taacttacga
     1321 taaaaatccg gatcgcgcgg tagcgatgaa tatgcgttta ctccgccttc ttcaacggta
     1381 ataaattgcg ggcgtaaata tccttcctca aatgccagaa agcggatccc tttcgacttt
     1441 gcccaacgtt ttgcttcttt gtgcaatggg cggcagtcac caaagcagag gatggtatca
     1501 aagtcatatt gccggtggag atcccgtaac catccgggga actctttcgg cgtttggtag
     1561 taagccaggt attgtcgatg gcggcagtaa aaacgatccc caccgttgaa tacaacattc
     1621 acagcgttac gtcctaatga ctctaaccat tcggcgacgt cattgaaaaa aggtcccatc
     1681 ggcccctgca atagcagata ttttttaccg gataataaaa cggttagtgc attaccttgc
     1741 ataaataatt tgcgtaatag tcaataatgt aattgtttag ccaaatctga ccttgcagaa
     1801 cataattaat ttgcggtaat agcgtattac tcgccccgct ttttttctgg taataaacat
     1861 cggcttgcgc ggcgtcttga ttaaatattc cgcagcttct tctaccgtaa taggttgtag
     1921 ccgtgttggg tggatatagg ttggataaac aatcagcgtc tgatatagca gatccgctaa
     1981 cgttaatttt cgctcgcgac gcgggcagcg atgttcatcg acggttaaac cccaaccggc
     2041 atagaagggc aggccgtaac aatgtacgtg cttgccatgt aataatgctt caaaccccga
     2101 cagcgacgtc atggtatgca cttcatctgc gcgttggatg cattgaataa tatctgcgtc
     2161 cagtgcctga taatcagcga gttcagcaat ctgttctgcc ggaatatcgc ccttgcgatt
     2221 ccccactaat acatccgggt gcggtttata aataatgtag gcgtgcggat tgcgctcgcg
     2281 tactgtgcgt aataactcaa ggttgctctt aatcgacaca gtgcctgttt taatagaggc
     2341 atcgtcctct acctgacccg gcaccaggat aactttttta tctttggctt cggctggtag
     2401 agagaaatcg gctcccaggt tgtacttact cagtttgctt tcaaccagtc gctggcgtaa
     2461 tttttcagct cgcatcttct gcgccagcgt tagctggtta tgattcagca gcacttccag
     2521 atcgctgggg cgcgtggcgt catagtaaat accgcgttta tccagcacca gcgatagcgg
     2581 cggcagcagg tcagagccaa gtccggatga acgcagaaaa ccatcttcca ttcgccataa
     2641 cggcagtgat tttcgctgcg cttcggctcg ccattgctgt tctcccttta caccccatac
     2701 cacgcaggcg ctcgccgcag tacagcgacg tgaaaaactc agccggtttg ttgccgtttg
     2761 caagaaaggt ttcaggatcg ctgacttcca cagcgttaag cctggcaccc ataaataacc
     2821 attacgctgt tgcagatgtc gacgttgtaa ttgcagccat tgcagcacgg taaatagatt
     2881 gcttacttct cccgtttgcg gatcgatata gcgacagtaa cgcaggtatg cagcggcaaa
     2941 aagttcctcc agcgtggcag aaccgcgtcg ggcagataac aaagcggact gcggatggcg
     3001 atcgtcggtt aagccccagc ctgcatacca gggctggccg aaacatgtta ctggttttcc
     3061 tgccagcaag gcttcaaagc cgtattggga tgtcacgacg taaacccggg aaacgtgtcg
     3121 caacagcgac tgcgggctga cattctcggc aatcaaacgt actcgttgcg tggcgcgcag
     3181 atcggcgaaa taacctgttt tctttccttc caggacatcc ggatgcacct tcacccaaat
     3241 ttcggcttga ggattttccg ccatcgcggc ttccagcatg gcagcaaact catgcgggcc
     3301 agcattgcca tacgtcactg acatatcatt aaatgtctga tcgacaacca gaacgatgtc
     3361 tgaacgttct gactcatcag ctacaaacgc aggtgccagg ttatatttcg acaaatcccc
     3421 ggtcacgatg gtgtgcatcg cttctctggc ctgatcagcc aatgccgcat ttccggcttt
     3481 atcctgtacc agtttctcca gcgccgaagg cttgctggca tcgtagtaaa tgccacaatc
     3541 atccaccacc agagaaagcg gcggctcgcc attgacgcca agatccagcg aacgcacaaa
     3601 tccatcttcc agacgaatga cgggttttcc cgctgctttg gcgatggcga ctggtttcgc
     3661 cgcgctggga cgatgtcccc acacggcgat agcatcaact tcttgcggaa cagggcgcag
     3721 cagagaaagt ttctggcacg gttgcgccag aaatttctcc agatgcggaa tacgccagat
     3781 gccaggcgag taaatgccaa tcatgcgttt tcagccagtt cctgtgccat cagggcgcgc
     3841 actttttcca ggcatgccgg ggtgtcgacg cccggaccgg ttgcggcaac ctcaaatgta
     3901 cggatgttga tccccgcgct catcaaccgc agctgctcca gtgattctgc ctgctcctgc
     3961 atggactccg gtaactggct gtagttttgc agcacatcgc gacgataagc gtaaatacca
     4021 acgtgtttca ggtagcgcgc tttttcagca ttacgcggat acggaatagg cgaacggctg
     4081 aaataaagcg catcctggtg ggtatttacc accactttta ccgtgcttgg ctcggtcgct
     4141 tcttcggcag aaatcgcgtg gcatagcgtt gccactggca acgcggggtc atcacgcatt
     4201 ccttgtaaca gcgtttctac atcccgcggg cgaatcattg gttcgtcgcc ctgcaggtta
     4261 atgtagatat ctgcctcgac tttatgcatc acctcgacca gccgatcggt gccggattca
     4321 tgatcgttgc gcgtcatgat ggctttcccg ccaaatgcct gcacggcctt ttcaacacgc
     4381 tgatcgtctg ttgccaccca aacttccgca acgcccgcca cctgtaacgc acgctcgtaa
     4441 acatgctgga tcatcggttt accaacaata tcgagcagtg gcttacccgg caggcgcgat
     4501 gagccataac gagccggaat gacaataact gctttgctca tttcaactcc ttacaaagac
     4561 agaatcactt ttgcacccac cgccagctga tagaggatgg tggaaatacc acgggtaact
     4621 tcaatgtttt tcgattcata tttcggcaga accataatct catcgcccgg tttgagtgaa
     4681 tccacatctt ctgcgttgac ggctgcaccg ttctgacgaa tgacgataat tctggcgtta
     4741 cccgatttct gcgtcaggcc accacatttc tcgatgtaat cctcggtggt catacccttc
     4801 tgccagctca ccgcgttcgg gaacagcact tcgccatgaa ccataaccag cgatgttttc
     4861 tccggaatat tgatgacgtc gccatcttca agcagaacag aatcaatatt ggattcgttg
     4921 aggatcactt cacctttcgg aacaacggtg cgtgctttcg ccacaaagcg gctgatcagt
     4981 tgcgcttcct gcattcgcag gctggcttct tctttggtgg aggactgggc ggaaagtgat
     5041 gcttcctcca gcttttgcag cgagagattc agcatctctt tctgacgctg agccactgat
     5101 gggcgataaa gctgaactgc gttcatctgc gacatgctgt tcgggcggac tttttccaga
     5161 accgcacgca tagtggaacc ataaggcagt accatggcat gttcaccgga gtgtgcgcct
     5221 tcaacccgca cctgaatggt gccggcatag cggtcagtgc tcacgattaa ggtatcgcca
     5281 ttttgcaaca tacggcctgg cgcagaactg atcggatagt attcgctgcg tttttgcagc
     5341 ccctgtttac gcataatcgt aatgtgagtc gcgccaggct tagggcgcgc ccagctcaac
     5401 gcttccgtta cgggaatgct gctttcgcgg aactcaaagt cgtagctgtt aaagacatcg
     5461 ccctgaacgc tgaaagtatg ctgacgcggc ccgacgatga tggtgtcacc atcggcgaac
     5521 tgcgaaagcc ccagtttgcc gttcagcagg aagtcgtaca ggttgacgtt ggagcgcacg
     5581 cggttaccgc gcttgaccac aatatcaacg taacttccgc gctctggatc aacgccgcca
     5641 gccttgatca gataattgag taacgaatca gacgtcacgc cgccatacag accaggatta
     5701 cgcacaaatc cggtcacgta cacttttact ggctgcgcct gtaataagga ggcgtagacg
     5761 ttgacgttgg actggtatac ttccttcact ttggatgtga ccagggcatt tagctgacta
     5821 ttactgacgc cagcaacttt caccggacca acgttcggca ggaaaatatt acctttggga
     5881 tcaacctgta acgcaccatc aaaggtgaac gcaccccaca agcgaacctg aatgctatca
     5941 cccggattca gaatatagtc agggttgaat cctaccgtcg caccgctatc cgcgctggtg
     6001 ccgttgaaaa gttgagcacc aaacatacgg ctcattacca ccggcggtga gggcggcggg
     6061 gtattgtcga agccgctcat gttttgcgta tccgactgtt gcccgttcag aataccggta
     6121 agcggcgcgg ctcctgtaag gtttgggtca gcgttaatat caatggccgc gctggcctgc
     6181 gccgcgtgac aggcggcaat cagtaaaatt gatttaaata atttcatcac aaactcattc
     6241 agcgacagcg ttagtctcgg tgatcttcaa taacagccag caacagtttc agggtgccga
     6301 acagcaggca gcacaccagt aaccagcagg cgatcaaata agggatattt ggaaaagacg
     6361 attcctgcgg caactgtggc gaactgatca ctgacagcac cttgagctta cgagccgctt
     6421 ctacacgagt cttttcaatg gaggtcaggg tcagtttgta cagctcggtg ttgaactcta
     6481 cttttgattt gatttcttca aaatccactg ccatacggtt tagcttgtca ccctgtggcg
     6541 cagtgatttt gctttgttct tcatcgattt gtgcctgcaa tgactgaatc gcattacgcg
     6601 cactcactac ttgcggggcg tcctcacgca gatacgtcag caagttacgc agatccgctt
     6661 ccatctggat cttctggccc atcagcgtat tgactaacgt gctcgccgcc tgtgcctgtg
     6721 cctgtggatc cagaacgttg ttgttgtcct gataagagag caattccgct ttgctggcgt
     6781 ccagacgctg gcgtgccttt tccatctccg tttctgcaaa ggcaagctgg tcacgcgcga
     6841 tgcgatgtga catctcattg ataaagcgct ctgactcttt cagcacggtc tggttaaact
     6901 taagcgcaaa ctccgggcta aagccctgcg tctgaatatt cagtaatccg gttttatcgt
     6961 catacgagac gttgatacgg tccttgtagt acttcaggaa tccttctgcg gtttcatcct
     7021 tgctaagatg attaagaaaa tcgagcccgc tgtggctaaa tgcttcacga aaatttagtt
     7081 gcttatccag cgccgccagc atatccggcg agttgatgta ctctttcaga tacaaagcat
     7141 cttctgcgga actggggtta gaggcaccca gaagcagacc aaaattcagg ctgccgctgt
     7201 ttaaatcatc cgagcgttta atggcgactt tcgactcgct catatagcga ggctggctga
     7261 agatagccag atagatcagc agcaccgcca tcggtgccag aatgatgatt ttcgccaggt
     7321 gtttttggat atctgccagt gagatggcag acagacgagc acgcatccag gatacggcag
     7381 acttcacttt tatcaacatg taacgattat caaagtttca gtggggcgca aactcaatga
     7441 gagactaacc tctcgaaccc cgttgcttaa ttagtcgaaa atgcgcacaa ggccagtgac
     7501 tttatttgcc ttgttggtca ccaataacgt tgagacacgg tgtttttgca ttttttcttc
     7561 cgcttcaatg atcatggtgt cttccggtag cgtcagcggt tcacgtgtca tcatctgcgc
     7621 agccgtggcg gatgtcagag aatcctcttt ttccataaag cgacgcaggt caccgtcggt
     7681 gataatgccc gctagcccac cttctgcgtc ttctaccatc accattccct ggcatccgct
     7741 ggtgatacgt tgaatcacgg ttttaaatga cgcatccagc tgtaccgcag gaacatcatg
     7801 ctgcatgaca tcagcaacgc gggtcagcag gcgacgacct aatgaaccgc ccgggtgata
     7861 gcgcgcaaaa tcattcggca taaatttgcg ttggcggatc atggcaatcg ccagcgcatc
     7921 gccgatcgcc atcgtcagcg tggtagacgt tgttggtgca agattattcg ggcaggtttc
     7981 attcgccata tggagttcca gcacggcatc agcatttttc gccagcgtgg aatttccatt
     8041 attggtaatg gcgataattc ggttgccgaa atttttcagt gaaggaacta gcttgaggat
     8101 ttcatccgtt tcaccgctgg cagaaataag gatcagaaga tcgtaaggcg taatcatgcc
     8161 cagatcgcca tggaaagctt ctgcaggatg aataaagaaa ctaggcgtac ccgtagaggc
     8221 cagcgtcgct gacattttac gaccgacatg ccctgattta cccattcccg aaagaataac
     8281 gtgcccttta cagttcatta tcaaattaag gacacgctgg tactgcccgg aatccagttg
     8341 tttagacaag ttttgtaatg ctgcgccttg ttctgccaga gtctggcgaa cagaggtaat
     8401 tagatatgga tcgatagtac tgctctggtc atcaggtaaa tgtctttcag acatatcagc
     8461 tcctttgcac ggaaataatg ccacagtcac aacaaacccg aacccactat aatttattta
     8521 tgctaaatca atgggttgta acaaaatttt taatgaatat aaaaccatgg tcagtccatg
     8581 cttatatgca ggacttatca ccttcttatt aagtcgcaat gtctcatgga ggtgcgaaga
     8641 tgctgcaata ttacaggcga gaaggggaac caaattttgc ccacctattt aacacacccc
     8701 tacattatga acaaggaatt taggattttt atcatttatt aaaatgtttc tcacttgact
     8761 attaagacca aaaacaattt atcaattgat tatttcctcg taaaaaaaga acgtatgacc
     8821 atgttattaa taaataaaac acgtaaataa tggtgatgtt gttttgtgat taactattta
     8881 aagcggcata ataaacagat tgacaatttc tatgtaaaga agaggaaaat aataaaattt
//
LOCUS       DAZFFE010000151         8471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFFE010000151 DAZFFE010000000
VERSION     DAZFFE010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.151"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(153..229)
                     /locus_tag="ACHJ69_004347"
     tRNA            complement(153..229)
                     /locus_tag="ACHJ69_004347"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(193..195),aa:Asp,seq:gtc)
     gene            complement(362..1093)
                     /gene="dnaQ"
                     /locus_tag="ACHJ69_004348"
     CDS             complement(362..1093)
                     /gene="dnaQ"
                     /locus_tag="ACHJ69_004348"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO5017200.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNATF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1158..1625
                     /gene="rnhA"
                     /locus_tag="ACHJ69_004349"
     CDS             1158..1625
                     /gene="rnhA"
                     /locus_tag="ACHJ69_004349"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO5017201.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGHEKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDTALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1622..2344)
                     /locus_tag="ACHJ69_004350"
     CDS             complement(1622..2344)
                     /locus_tag="ACHJ69_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5017202.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2378..3133
                     /gene="gloB"
                     /locus_tag="ACHJ69_004351"
     CDS             2378..3133
                     /gene="gloB"
                     /locus_tag="ACHJ69_004351"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO5017203.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAISANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3205..4563
                     /gene="mltD"
                     /locus_tag="ACHJ69_004352"
     CDS             3205..4563
                     /gene="mltD"
                     /locus_tag="ACHJ69_004352"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO5017204.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4612..5370)
                     /locus_tag="ACHJ69_004353"
     CDS             complement(4612..5370)
                     /locus_tag="ACHJ69_004353"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5017205.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLADYPDASV
                     LDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEARQLKNITTRQGYVESLPF
                     ADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGLLIVMDVMSPGHPVRDIWLQTVE
                     ALRDTSHIRNHASGEWLTLISEANLIVDNLITDKLPLEFSSWVARMRTPEALVDAIRI
                     YQQSASTEVKMYFALQNDGSFTSDIIMLEAHKAA"
     gene            complement(5448..6248)
                     /locus_tag="ACHJ69_004354"
     CDS             complement(5448..6248)
                     /locus_tag="ACHJ69_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO5017206.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6489..7403
                     /gene="yafC"
                     /locus_tag="ACHJ69_004355"
     CDS             6489..7403
                     /gene="yafC"
                     /locus_tag="ACHJ69_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO5017207.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFHRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISCYGKPETIDDLKQHICLRFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSGHVKTAPGGAVREA"
     gene            complement(7400..8203)
                     /gene="dkgB"
                     /locus_tag="ACHJ69_004356"
     CDS             complement(7400..8203)
                     /gene="dkgB"
                     /locus_tag="ACHJ69_004356"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO5017208.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELDYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPS
                     DEVSAEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGNALKDKVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(8366..8442)
                     /locus_tag="ACHJ69_004357"
     tRNA            complement(8366..8442)
                     /locus_tag="ACHJ69_004357"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8406..8408),aa:Asp,seq:gtc)
BASE COUNT         2267 a         2026 c         2050 g         2128 t
ORIGIN      
        1 atgattaaat attaatgata tggtctatca tatgtgatta acacatttac agcataacaa
       61 aatcatagac caaagaaata gcgggattta gatgaaaaaa ccgcaaaatt aaaaatcttg
      121 cggttctctg aactcatttt catgagtgaa tttggcggaa cggacgggac tcgaacccgc
      181 gaccccctgc gtgacaggca ggtattctaa ccgactgaac taccgctccg cgttgtgttc
      241 cgttgggaac gaggcgaata ttacggattg cctcaccctt cgtcaacggt ttttctcatc
      301 ttttgaatcg tttgctgcaa aaatcgccca agtcgctatt tttagcgcct tttacaggta
      361 tttatgcccg ccagaggcag cttccgccct tcttctgcac cagatcgaga cgggcttcat
      421 gtgctgcaag ctcttcatct gtcgcaaaaa caacgcgtaa cttacttgcc tgacgcacaa
      481 tgcgctgaat tgttgtttca ccttgttgct gttgtgtctc tccttccatc gcaaaagcca
      541 tcgacgtttg accacctgtc atcgccagat aaacttccgc aaggatctgg gcatcgagta
      601 atgccccatg cagcgttcgt ttactgttat ctatttcgta gcgggcacat aacgcatcga
      661 ggctgttgcg tttaccggga aacattttcc tcgccaccgc aaggctatcg gtgaccttac
      721 agaaggtatt ggtcttcgga atatcgcgct taagcaacga aaactcgtag tccataaagc
      781 cgatatcgaa cgttgcgtta tggatcacca gctccgctcc gcggatatag tccatgaact
      841 catcggctac ctcggcaaac gtgggcttat cgagcaaaaa ttcatcggca ataccatgta
      901 cgccaaaggc ttccggatcc accagccgat cgggtttgag ataaacatgg aagttattgc
      961 ccgtcaggcg acggttcacc acttcaacgg caccaatctc aatgatcttg tggccttcat
     1021 agtgcgcacc aatctggttc ataccggtgg tttcggtatc gagaacgatc tggcgtgtaa
     1081 ttgcagtgct catagcggtc atttatgtca gacttgtcgt tttacagttc gattcaatta
     1141 caggaagtct accagagatg cttaaacagg tagaaatttt caccgatggt tcgtgtctgg
     1201 gtaatcctgg acctgggggt tacggcgcta ttttacgcta tcgcggacac gagaaaacct
     1261 ttagcgctgg ctacacccgc accaccaaca accgtatgga gttgatggcc gctattgtcg
     1321 cgctggaggc gttaaaagaa cattgcgaag tcattttgag taccgacagc cagtatgtcc
     1381 gccagggtat cacccagtgg atccataact ggaaaaaacg tggctggaaa acagcagaca
     1441 aaaaaccagt aaaaaatgtc gatctctggc aacgtcttga tactgctctg gggcagcatc
     1501 aaatcaaatg ggaatgggtt aaaggccatg ccggacaccc ggaaaacgaa cgctgtgatg
     1561 aactggctcg tgctgcggca atgaatccca cactggaaga tacaggctat caagtcgaag
     1621 tttaagcctg tggtttacga cattgccggg tggcaccaac cgcctggcga attcgcggtt
     1681 tattcttgct ctgtttcatc ggatttagcg ttaaaggaat agtccgtttc cgggcaacaa
     1741 taagttgtaa gcaaccaagc gcaggaatat gcgcattcaa tagttttcct ccgtgtttgt
     1801 tccacgggag aacgtggaaa cggctggcgt gtagcacttc aaaattcaac aaagagagcc
     1861 agtccagctg ccgcatcaga gtaaacatcc ggctgttata gggcgatgtt ttgcgcaata
     1921 ccggcacaag tttgcgtaat cccattaaac tgatgggatt gaagccacta atcaccagcc
     1981 aaccatcatc aatcagtacc cgatcggctt cacgcaataa acgatgcgga tcagtgcacc
     2041 acggcaatgt atgtgccagt agacaaacat caacggattt atcggcaaaa ggaagatgaa
     2101 gtgggtccgc ctgcacctgg acgggcattc cttgcgcaga aacattcact tgatgagaaa
     2161 ccgcgcacgc ttcgcaattg atttctgcgc ttaaattgcc aatcttaagc agatgaaaac
     2221 catacatttt agtgaaccac gggttgagct gacgctccag cgccttgcga taaagctctc
     2281 cccagggcaa atcgccccag caatcaggag ccacgacagt ttgagggact cttgccggtt
     2341 tcatcacaac cttccgtttc acactgagag gtaatctatg aatcttaaca gtattcccgc
     2401 ctttgatgac aattacatct gggttttgaa tgatgaagca ggtcgctgtc tgattgtcga
     2461 tcccggggac gcagagccag tattaaacgc catttccgcc aataactggc aaccggaggc
     2521 catatttctc acccaccatc accacgatca cgttggcggc gtaaaagaac tggtggaaaa
     2581 gtttccgcaa attgtggtgt atggaccaca agagacacaa gataagggaa caacacaggt
     2641 agtcaaagat ggcgaaactg ccttcgtttt ggggcatgaa tttagtgtaa ttgccacacc
     2701 gggtcacact ttaggacata tctgttactt cagtaaacct tatctatttt gcggcgacac
     2761 gctgttttct ggtgggtgtg gtcggttgtt tgaagggaca ccatcacaaa tgtatcaatc
     2821 acttaaaaag ttaagtgcgt tacctgacga cacattggta tgttgtgctc atgaatatac
     2881 cttatcaaat atgaagtttg ctttgagtat tcttccgcac gatttgtcca taaatgatta
     2941 ttatcgtaaa gttaaggagt tacgggcaaa aaatcaaata acactacccg taattctgaa
     3001 aaatgagcgg caaattaatg tttttttaag aacggaagat attgatttaa ttaatgtaat
     3061 taatgaagaa acattattgc aacaacctga agagcgtttt gcatggttaa ggtcaaagaa
     3121 agataggttc tgataaaact ttcttgtcat cggctccgtt cgccgttatg atcggtcgtc
     3181 ttttaagcaa ctattgacac acacatgaag gcaaaagcga tattactcgc ctctgtcctg
     3241 ctcgtgggtt gccagagcac cggcaacgtt caacagcacg cacagagcct ttctgcagct
     3301 ggtcaagggg aagcagcaaa gtttacaagt caggcacgat ggatggacga tgggacgtct
     3361 atcgcgccag atggtgactt gtgggctttc attggcgacg agctaaagat gggaattccg
     3421 gaaaacgacc ggattcgcga acagaaacag aaatatttac gcaataagag ctatctccac
     3481 gatgtaactt tacgggcaga gccgtatatg tactggatag ccgggcaagt taaaaaacgt
     3541 aacatgccta tggaactggt actactaccc atagtggaga gcgcttttga tcctcacgca
     3601 acgtctggcg ccaatgccgc gggcatctgg cagatcattc cgagcacggg gcgcaattat
     3661 ggtttgaaac agacccgcaa ttatgacgcg cgtcgcgatg ttgttgcttc aacaactgcc
     3721 gcgctgaaca tgatgcagcg tctgaacaag atgtttgacg gcgactggct tctgaccgta
     3781 gcggcttata acagcggcga aggtcgggtc atgaaggcaa ttaaaacgaa caaagcgcgt
     3841 gggaaatcca cggacttctg gtcgttaccg ttgccgcagg aaacgaagca gtacgtgcct
     3901 aaaatgctgg cattgagtga tattctcaaa aacagcaagc gttatggcgt acgtctgcca
     3961 acgaccgatg aaagccgtgc tctggcacgt gtgcacctga gcagcccggt tgaaatggcg
     4021 aaggttgcag atatggcggg gatttccgtc agcaagctga agacattcaa cgcaggcgtg
     4081 aaaggctcca cgctgggcgc aagtggtccg cagtacgtga tggtgccaaa gaagcatgca
     4141 gatcaactgc gtgaatctct ggcttcaggc gaaattgctg ctgtacagtc gacgctggtt
     4201 gccgacaata cgccgcttaa cagccgtgtt tacaccgtac gctctggcga cacgctttca
     4261 agtatcgctt cacgtctcgg cgtaagcacc aaagatttgc agcagtggaa caaactgcgc
     4321 ggatctaagc tgaagccagg ccaaagtttg acgattggtg caggcagtag cgcacagcga
     4381 ctggcaaaca acagcgatag cattacgtat cgtgtgcgca aaggcgattc gctttcaagc
     4441 attgctaaac gccacggcgt gaacatcaaa gatgtaatgc gctggaacag cgacactgcg
     4501 aatctgcaac caggcgataa gctgacgttg tttgtgaaaa acaacaacat gccagattcc
     4561 tgacaaacca gataataaaa aaggcaccga ttcccccggt gcctttttta tttatgccgc
     4621 tttatgtgct tctagcatga tgatatcact ggtgaaagag ccatcattct gcaaggcaaa
     4681 atacattttc acctctgtcg atgcgctctg ttggtaaatg cgaatagcgt ctactaacgc
     4741 ttctggcgta cgcattctcg ctacccatga agaaaattcc agcggtaact tatctgtaat
     4801 taaattatca actatcagat tggcttcact gattaacgtc aaccactcac cgctggcgtg
     4861 gttccgtatg tgagatgtat cgcgtaatgc ttctaccgtc tgtaaccaga tgtcgcgcac
     4921 tgggtgaccc ggagacatta cgtccatcac aatcagcaga ccaccgggtt tcaatatccg
     4981 gttcacttca cgcagtgctg caccaacatc atgccagtga tgggcagaat aacggctgat
     5041 aacaatatca aatgcgttat cggcaaatgg caggctttcg acataaccct ggcgggtggt
     5101 gatatttttc agttgccggg cttcggcagc ttgtgccacg acatccagca tatgggcaga
     5161 taagtcatac gccaccaccg tgctcacgtt ttgcgcagcg acaaagctgg catgccctgc
     5221 tccgcagccc atatcaagta cacttgcatc aggataatca gccagacgct gtaaatctcg
     5281 tccggatgca tgtacggtac tagtcagata ttcgctagct tgagagctaa actgcttttc
     5341 tacgtggtca tggtgggatt gtgttgtcat tgcgctgtcc ttttgttatt tgagtataaa
     5401 agggcagcgc caaacgttgg tctgccctgt ggcagacctg acatacctta tttatcaggc
     5461 ttgccgggac tgaattcaac gagtagcgga ttgtgatcgg acgcgcgcgt aaccagtaca
     5521 gaagcttcgc tgacgttcag accacggtag aaaacaaaat cgagcgggcg accaaacgcc
     5581 cgacggcgct gatcatcggt aaaacgcacc tggcgcagcg acatttcccg tgcaaagcga
     5641 tataacgcgt tcatccttct acggctccag gcattgaaat ctcccgccat aatgaccggg
     5701 ccactgtggt gagctatctg atcgccaata ggaagtaact gcttactata gacatccacg
     5761 cccagactga agttgacggc gtgtatatta accaccatca acaggcgggt gtcaggtaat
     5821 ggatagaccg tcaccagtgc cgacttcgcc agacgcaaaa tgggttctcg ttcacgtaac
     5881 gggcagcaat acactggatg tgccgccgaa agggtcatta cgccagaagg atgttgtggc
     5941 agcacgaaag cgggtacctg atcggcggca agatagttag cagtcgcaaa ctgtactaac
     6001 tctggcgttg tctgcgcttc ctgcaataac accagatgtg catccttgcc gtagttcttt
     6061 aataccgaca accattcagc gcgttgctgt ttgtatatgt tccacaccag gatccgaata
     6121 cgctcttcgg tacttaacgg ttctccaggt ggtaatgcct ggccgatgct cgcaaaagac
     6181 cctggcggta agatcctttc cgcaggttgt ccggcaacat agcgcatggc ataggtgttt
     6241 tttcgcactt ttgactttga aacctctatc acgtcaaccc ttccgcactc gggagggttc
     6301 tactgttata gggattttag ctcactcttt caacgagcaa ttgtatattg ttatgtaagc
     6361 aagtgcttac accggtcagg attttgctca ttattgagaa cattaaatcg gatggcatat
     6421 ctcgcatcct gatggtttaa tgacaaataa attcaaaata tctcttctat tttgcaacag
     6481 gagcaaaaat gaaagccacg tcggaagaac tcgccatttt tgtttcagtc gtcgaaagcg
     6541 gcagctttag ccgggcagcg gaacaattag ggcaagcaaa ctcagcggta agccgggcgg
     6601 tgaaaaagct ggagatgaaa cttggcgtta gcctgcttaa tcggaccacg cggcaactta
     6661 gcctgacgga agaaggcgag cgttattttc atcgcgtaca gtcaattttg caggagatgg
     6721 cagcggcaga atcagaaatt atggagacgc gtaatacacc gcgtggactg ttacggatcg
     6781 atgccgcaac tccagtggtg ctgcactttc tgatgccgtt gattaagcct tttcgtgaac
     6841 gctatccgga agtcacttta tcgctagtct cctccgaaac gattattaat ttgatcgaaa
     6901 gaaaagtgga tgtcgcgata cgcgccggta cgttaacgga ttccagctta cgtgccaggc
     6961 cgttatttaa cagttatcga aaaattatcg cctcccccga ttatatttcc tgctacggga
     7021 agccagaaac gatcgacgat ttaaagcaac atatttgcct gagattcact gaacccgctt
     7081 ccctcaatac ctggccgata gcctgtagcg atggacaatt acatgaggtg aagtacggtt
     7141 tgtcatccaa tagtggggaa acactgaaac agctttgcct gagtggcaac gggattgcgt
     7201 gtttgtccga ctacatgatc gacaaagaaa tcgctcgcgg agaattggtg gagttaatgg
     7261 cagataaagt gttgccagtg gaaatgccat tcagtgcagt ctattacagc gaccgtgcgg
     7321 taagtacgcg catccgggct tttatcgatt tccttagcgg acatgtaaaa acagctcccg
     7381 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
     7441 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
     7501 gtgccttaag attactctcc aagttttcac gtttagtaga agaaggaatt actgagtaac
     7561 cttcccccat agcccacgcc agaatcactt gagccggagt cgcattgtgt ttagctgcaa
     7621 tacgagcaat aaccttatct ttcagtgcat tgccatacgc cagagtcata taggaagtaa
     7681 tatggatacc gtgctgttta gcccaagcga ccactttacg gttttgcaga taaggagaga
     7741 gttcaatctg gttagtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
     7801 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
     7861 gcagcgcctg cataaactct tcagcagaga cttcatcgct tggtgacggc cagtggatta
     7921 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
     7981 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
     8041 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
     8101 tatcaattgc gcgataatca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
     8161 ctttcagacg gaaagtgcct aaaccaaatg cagggatagc cataatatgc ctcttttaat
     8221 gctattttcg ttaactgagg tgattatggg cttcagacag ttgtggaaaa atggcgtaaa
     8281 acgcagagga tttttgcgat tttggcaata atagaaatac aaacgaaaaa aaacctcgag
     8341 cgttaactcg aggcttctta ataagtggcg gaacggacgg gactcgaacc cgcgaccccc
     8401 tgcgtgacag gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac
     8461 caccggttta t
//
LOCUS       DAZFFE010000152         8448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFFE010000152 DAZFFE010000000
VERSION     DAZFFE010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.152"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(84..890)
                     /gene="trpA"
                     /locus_tag="ACHJ69_004358"
     CDS             complement(84..890)
                     /gene="trpA"
                     /locus_tag="ACHJ69_004358"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO5017209.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAECEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNAAD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVTKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            complement(890..2083)
                     /gene="trpB"
                     /locus_tag="ACHJ69_004359"
     CDS             complement(890..2083)
                     /gene="trpB"
                     /locus_tag="ACHJ69_004359"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO5017210.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGHLPDAVIACVGGGSNAIGMFADFINETDVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2095..3453)
                     /gene="trpCF"
                     /locus_tag="ACHJ69_004360"
     CDS             complement(2095..3453)
                     /gene="trpCF"
                     /locus_tag="ACHJ69_004360"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO5017211.1"
                     /translation="MQTVLAKIVADKAIWVEARKQQQPLASFQNEIQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLPI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLHDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPARDFQHIDKYVFDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            complement(3457..5052)
                     /gene="trpD"
                     /locus_tag="ACHJ69_004361"
     CDS             complement(3457..5052)
                     /gene="trpD"
                     /locus_tag="ACHJ69_004361"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO5017212.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5052..6614)
                     /gene="trpE"
                     /locus_tag="ACHJ69_004362"
     CDS             complement(5052..6614)
                     /gene="trpE"
                     /locus_tag="ACHJ69_004362"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO5017213.1"
                     /translation="MQTQKPTLELLTCKGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALSDTVTIQALSGNGEALLTLLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFEN
                     LPQLSAENSCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6636..6729)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6706..6750)
                     /gene="trpL"
                     /locus_tag="ACHJ69_004363"
     CDS             complement(6706..6750)
                     /gene="trpL"
                     /locus_tag="ACHJ69_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO5017214.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6888..7769
                     /gene="rnm"
                     /locus_tag="ACHJ69_004364"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6888..7769
                     /gene="rnm"
                     /locus_tag="ACHJ69_004364"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO5017215.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQSIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7766..8386
                     /gene="yciO"
                     /locus_tag="ACHJ69_004365"
     CDS             7766..8386
                     /gene="yciO"
                     /locus_tag="ACHJ69_004365"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO5017216.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
BASE COUNT         1977 a         2253 c         2250 g         1968 t
ORIGIN      
        1 gataaataat aaatgaattt aggatttttc cggcttcgtt aaagaaagtt aaaatgccgc
       61 cagcggaact ggcggctgtg ggattaactg cgcgtcgccg ctttcatcgg ttgtacaaaa
      121 gctttcagtg ccgccagcat tttctctggc tcattaatat gttgctcgat gattttaacg
      181 atggccgaac cagaaatcgc gcccgcagct cctgcatcaa tcgctgcttt tacctgatcc
      241 ggggcggaaa taccaaatcc ctgcaatgga ggcgcagcgt tgtactcttt cagcttcgtg
      301 accagatgat tgaggggtaa cgcggcgcgg ttttctgcgc cggtcacgcc cgcacgtgac
      361 aataaatagg tgtatccacg accgtaggag gctatctggc gcagcaaatc atcggcggca
      421 ttcggcgggc aaataaagat aggtgcgaca ttatgacgca acgcggcctg gcggaagggc
      481 gcggactctt ccacgggcac atcggcaacc agcaccgaat cgacgccgac tttctcgcac
      541 tcggcataaa actcatcaat gcctttgtta aacaccaggt tggcatacat caaaaggccg
      601 atgggaatgg tcgggtgctt ctggcgaatg agtgccagca tctcaaagca ctgcgccggg
      661 gttactcccg ccgcaaaagc acgcagtgtg gcgttttgaa tcgtcgggcc atccgccagt
      721 gggtcggaga aggggatgcc taactccagc gcgtcagcac cggcttcaat tagcgtatcg
      781 ataattttca acgactgctc aatgcccgga tcaccgaggg tgacgaaagg aacgaatgcg
      841 ccttctttgc gctccttcaa ctgggcaaac agagattcgt agcgttccat cagatttccc
      901 ctcgtgcttt caaaatatcg tgaacggtga agatgtcttt atcgccgcga ccggaaaggt
      961 taaccaccag tagctgctct ttttccggat tttcgcgcat cattttcagc gcatgggcca
     1021 gggcgtggga ggattccagc gccgggatga tcccttcatg caggcaaagc gttttaaagg
     1081 cttccagggc ttcatcgtcg gtaatagaca cgtaatcagc gcgtccagtg ctgttgagat
     1141 acgcatgttg cgggccgacg gacgggaaat ccagcccggc agaaatggag taagactctt
     1201 caatttgccc gtcttcggtt tgcatcatcg gcgctttcat accgaaatag atgcccacgc
     1261 gaccatgttt taacggtgcg ccgtgctcgc cagtttcgat accgtggccg ccaggctcca
     1321 caccaatcag gccgacgtcg gtttcgttga tgaaatctgc aaacataccg atggcattcg
     1381 aaccaccgcc aacacaggcg ataacggcat ccggcaggtg accttctctt tccagaatct
     1441 gcgctttcgt ttcttcgcca atcatccgct gaaactcacg cacaatggtc gggtaaggat
     1501 gcgggccagc tgcggtaccc agcatatagt gcgcggtttc ataactgccg gaccagtcgc
     1561 gtagcgcctc attacaggca tctttcaggg tcgcggaacc gctatgtacc gggatcactt
     1621 ccgcacccat taagcgcatc cggaaaacgt taggcgactg gcgttcaacg tctttggcac
     1681 ccatataaat acggcatttc aggccgagca gggcgctggc aagggccgac gccacgccat
     1741 gctgaccggc accggtttcg gcaataattt cagttttacc catccgcttc gccagtaaag
     1801 cctgaccgag tacctggtta gttttatgcg cgccgccgtg cagcaaatct tcgcgcttca
     1861 gatacagcgt ggtgttcgtc ccggctgtaa tgttctggca tttggtcagc gcggttggac
     1921 gcccggcata gtttttcagc aggtcgttga actgagcctg aaattcagga tctttttgcg
     1981 cgctaacaaa agcttcttcc agctggcgca gagcaggcat caggatttgt ggcacgtaca
     2041 tgccgccaaa ctcaccaaaa taggggttaa gtaatgttgt cattgttcct ttccttaata
     2101 tgcgcgcagc gtctggaaaa ccgaggccaa aagacgtgcg tctttgatac ccggttgcga
     2161 ctctacagca gaattaaaat caagcccggc gcagccggtt tgtgccgctt ccacgcagtt
     2221 atctgcgcct aagccccccg ccagcagaac gttgccaagc gattgaccat ttaatagtga
     2281 ccagtcgaaa cgttgtccgc tcccgccctg accgttgtcg aatacatatt tatcgatgtg
     2341 ctgaaaatcg cgcgcgggaa gagtttcacc gacacttaaa gccttccaga tggcgacgtg
     2401 tgctggcaga gcctcacgca gagtgtcgat atacagctga tcttcattac catgcagttg
     2461 cactgccgcc agcgataaca ccttagcttt gtccaccaca tcggcaatat cgtgattgcg
     2521 gaacacgcca acatactgca acggtgccgc agccatcact tcctgcgcct gttcaacgtt
     2581 gacgcaacgc ggtgatgtcg caacaaaaat caacccaccg taaatcgcgc ccgcgtcata
     2641 agctgcttta gcatcttgcc cacgtgtcag gccacatact ttattctcac ccagcaacac
     2701 ccgacgcacg gcggcgttca aatcgtcatg ggccatcaac gccgaaccaa tcagaaagcc
     2761 gttagcgaag tggcttaact cgcgcacctg agcgtaagta ttgatgccgg attcgctgat
     2821 taccgtcacg ttgtgcccca gtttcggcgc aagctcgcgg gtacggttga gatcaatcga
     2881 caaatcgtgc agatcgcggt tgttgatgcc aacgaccttt gcccccaatg caatggcgcg
     2941 ctccagttcc tcttcattac tgacttcggt cagcacaccc atctccagac tgtgggcgac
     3001 ggctgcaagc tggcgatatt gttcgtcatc cagtactgaa agcattaata agcaggcatc
     3061 ggcctggtaa tagcgcgcca gatagatctg gtaaggatcg ataatgaagt ctttacataa
     3121 aatcggctgc ggggcgattt ggctgacgat ggggaggaaa tcaaagctcc cctgaaaata
     3181 tttctcatca gtcagcactg aaattgccga agcgtaatgt ttataaatgg cagcaatgcg
     3241 tgccggatcg aaatcatcac ggatcacgcc ttttgacggc gacgcttttt tgcactccag
     3301 aataaacgcc gtgcgtgcac cctgtagcgc atcataaaaa tgtcgcgtgc tcggctgaat
     3361 ctcattctga aaactggcca gcggttgctg ctgtttgcgg gcttctaccc aaatcgcctt
     3421 gtctgcgacg attttcgcta aaacggtttg cattatttac cctcgtgccg ccagtgcggt
     3481 gactctgtcg taagcggaac cactgcgcag tacctcaaga acggtttgcg cattggcttg
     3541 cagatcttca tggccatgca ggcgcattaa catggcgaca ttcgccgcga cggctgcttc
     3601 gtgagcggcg tcgcctttac cttgtaacaa gcgtgttaaa atgtcacggt tttcttccgg
     3661 tgttccgcct gccagttgct cctggtggta gggtgtcagg ccaaaatctt cagcggtcaa
     3721 ttgatagctc ttaatttcgc cgtcatgcag ttcggcaacg attgtcggcg cgtgtaatga
     3781 aacttcatcc atcccgccgc tgtgcaccac cgccgcgcgt tgatacccca gcacgcgcaa
     3841 ggtttcggca atcggcagca ccagttccgg actataaaca ccaattaacg ccagcggcgg
     3901 atgcgccggg ttaatcaatg gccccagcac attgaacagt gtgcgggttt ttagttgctg
     3961 gcgaaccggc attgcatggc ggaatccggt gtgatatttc ggtgcaaaga ggaaacagac
     4021 gcctaactca tccagcgcct ggcgcgattt atcggcgttc atatcaagat taataccgaa
     4081 cgccgccagc agatccgacg agccggattt actggagacg ctacggttgc cgtgtttcgc
     4141 cactttcagc ccgcaggccg cggcgacaaa cgcactggcg gtagaaatat tgatgctgtt
     4201 actgccgtca ccgccggtgc cgacgatatc ggcaaacaga taatccgggc gcgggaacgg
     4261 cgcggcgttt tccagtagcg cggttgctgc tccggcgatt tcgttcgggt gctcgccgcg
     4321 aattttcatg ctcaccagcg ccgccgccag ttgttccggt ttcagttcgc cacgtaccac
     4381 cgctgaaaac agctggtggc tttcttgttg gctaagggtc tgcgcctgat acagtttttc
     4441 cagaatcggt tgcagcgtgt tggttggctc aagtttctgc tgcgcccagg ccagcgtttg
     4501 ttccagcagg cgagcgccct gggtagtaag aatggattcc ggatggaact ggaatccaca
     4561 aacgcgatcc gcatcatgac gcaccgccat caccatgcca ttaaaatggg cgttgatggt
     4621 taaaccggcc ggaatattac tgccaaccag cgagtgataa cgcgccactg gcagcgggtt
     4681 tgttaatccg gcaaacatcg cctgaccgtc atgttcaatg ctcgacgctt taccgtgaag
     4741 aatttcgccc gcctgaccga catagccccc gtaagcttcg acaatcgcct gatgtccgag
     4801 gcaaatgcca ataattggca gcttgccacg caagcgggtg aggagttccg gcatacaacc
     4861 ggcttcgctc ggcacaccgg ggccaggaga aagcatcagc accggattgc tcatcgtcgc
     4921 caggcgttca attaaggtct gcgccggaat atggttgcgg taaatcacca cgttatgacc
     4981 attgctgcgc aactgatctg ccaggttgta cgtaaaagag tcgatattat cgagcagcag
     5041 aatgtcagcc atcagaaggt ctcctgtgca tgatgcgcgg tggcaatagc gcgcagtaca
     5101 gcgcgggctt tattacgagt ttcgtcggct tccgactgcg gaacagaatc aaggactacg
     5161 ccagcaccgg cttgcacggt ggcgataccg ttttccacca gcgccgagcg gatcacaatg
     5221 caggtgtcga gatcgccatg cgcggtaaaa taacctaccg cgccgccgta gctgccgcgt
     5281 cgacgacctt ctgcttcggc aattaactgc atagcgcgta ctttcggtgc accgcttaac
     5341 gtccccatat tcatacaggc gcggtaagcg tgcagggcgt cgagatcgtg gcgcagctca
     5401 ccaacaacgc gggagaccag gtgcatcacg taagagtaac ggtcaacttt ggtgagatcg
     5461 gcgacgtagc ggctgccggg tgtgcaaatg cgtgccaggt cattacgggc gagatccacc
     5521 agcatcagat gttcagaaag ctctttatga tcggtacgca tctccagttc gatgcggctg
     5581 tcgaggtctc tgtccagcga accatcggca cgacgaccgc gtggacgtgt tccggcaatc
     5641 gggtaaatct caatctggcg gctggtggcg tcatacttga gcgaactttc cggcgacgcg
     5701 ccaaacaggg tgaaatcatt atcctgcata aaaaacatgt acgggctggg attactcttt
     5761 ttcagcacgt aataggctgc cagcggtgac gggcagggca gagagaaacg gcgagatggc
     5821 actacctgga aaatttctcc ggcgcgaatc gctttttgca acaaacgcac tacgccaccg
     5881 aactcttcat cgctctggtt acattcacaa cgcatatgcg gcacggaaac caccggcagc
     5941 ggcggcgcgg cttcggtcag ttgctgacgt agttcgttaa ggcgagcagt gagacgttgt
     6001 ttttcttctt cattcggagc aaacaggctg gcctgaatac gggtgctttt tttctgatgg
     6061 tcaatcacca tcagcgtttc agcgagataa aaacagaaat cagggcagct attttccgct
     6121 gacagttgcg gtaaattttc aaatcccgcc acaaggtcat aagagaacag gccgccgaag
     6181 aacattgctt ctcgttcttc cttcggtaca ttcaacagat tctgtaataa gcggaaagcg
     6241 tcaaaaaccg aaagggagca taagcgggcg tcttcatcca gcagtggact gacaggcggg
     6301 aagcgcagta cgcggcagtt tggtgattgt tcattttcca cacccgcagg caaggcgtta
     6361 tccagtagtg tcaacagggc ttctccattg ccggaaagcg cctggattgt gacagtgtca
     6421 cttaatgctg taatgcgcag cgcactgtct accagcagca ggctttttaa atcatctttg
     6481 ctgtcgatat ctgcggattc cagcagcagc gttgccgggc gatccccaca caactggtga
     6541 aaaagcgcag tcgggttgtc gcgataagcg cctttgcagg ttagcagttc gagagtcggt
     6601 ttttgtgttt gcattgttat tctctaattt tgttcaaaaa aaagcccgct cattaggcgg
     6661 gctgggtatc tgattgcttt acgcatggtg aatacactgc ccgtttcagg aagtgcgcca
     6721 ccaacctttc agtacgaaaa ttgctttcat tgtcgatacc ctttttacgt gaacttgtgt
     6781 actagttaac tagttcgata attaattgtc aacagctcat ttcagaatat ttgccagaac
     6841 cgttatgatg tcggcgcaaa aaacattatc cagaacggga gtgcgccttg agcgacacga
     6901 attacgcagt gatttacgac ctgcacagcc ataccacagc ttccgatggc tgcctgacgc
     6961 cagaagcatt ggtgcaccgt gcagtcgaga tgcgcgtcgg caccctggcg atcaccgacc
     7021 atgacaccac agctgcaatt gcaccagcaa gagaggaaat ttcacgttcg ggactggcgc
     7081 tgaatcttat tcctggcgtg gaaatttcca cggtctggga aaatcatgaa attcatattg
     7141 tcgggctgaa tattgatatt actcatccgc taatgtgcga gttcctcgcg cagcagacag
     7201 aacggcgcaa tcagcgggcg cagctgattg ccgaaagact tgaaaaagcg caaatacctg
     7261 gggcactgga aggcgcgcaa cgactggcgc agggcggtgc ggtgacgcgc ggtcattttg
     7321 cgcgtttcct tgtggagtgc ggaaaagcca gttcaatggc ggatgtcttt aaaaagtatc
     7381 tggcgcgcgg gaaaaccgga tacgttccgc cacagtggtg tacaatagag caatctattg
     7441 atgtcattca tcattctggc ggtaaggcgg tgctagctca tcctgggcgg tacaatctta
     7501 gtgctaaatg gctgaaaaga ttggttgcgc attttgccga acaccacggc gacgcgatgg
     7561 aagtcgcgca gtgccagcaa tcgcccaatg aacgcaccca gctggcgaca cttgcgcgtc
     7621 agcatcattt atgggcatca caaggatctg attttcatca gccatgcccg tggatcgaac
     7681 tgggtcgtaa actctggctg cccgcaggcg ttgaaggcgt ctggcagcta tgggaacagc
     7741 cgcagaacac cacagagagg gaattatgag ccagtttttt tatattcatc ctgataaccc
     7801 gcagcaacgc ctgatcaacc aggcggtgga gatcgtgcgt aaaggcgggg tgattgttta
     7861 tccaactgat tccggctatg cgctcggctg taaaattgaa gataaaaacg cgatggagcg
     7921 tatttgtcgt attcgccagc tgccggacgg tcacaacttt accctgatgt gtcgcgatct
     7981 ttctgaactc tcgacctatt catttgtcga taacgttgcg tttcgtttga tgaaaaacaa
     8041 cacgccaggc aactatacct tcatcctgaa ggggacgaag gaggtgccac gccgtctgtt
     8101 gcaggaaaaa cgcaaaacca tcggtatgcg tgtgccgtct aacccgattg cgcaggcgct
     8161 gcttgaagca ttgggcgaac cgatgctttc cacttcgcta atgctgccag gcagtgaatt
     8221 taccgaatcc gatccggaag aaattaaaga ccgtctggaa aaacaggtgg atttgattat
     8281 tcacggcggc tatctcgggc agaaaccaac aacggttatt gatctcaccg atgatacgcc
     8341 ggtcgtggtg cgtgaaggcg taggtgatgt gaagcctttc ttataacaaa ttgcgttcca
     8401 cggatggaag atgatggcag ggatatatcg ctgcattttg ctattgat
//
LOCUS       DAZFFE010000153         8396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFFE010000153 DAZFFE010000000
VERSION     DAZFFE010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.154"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..303)
                     /locus_tag="ACHJ69_004366"
     CDS             complement(<1..303)
                     /locus_tag="ACHJ69_004366"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017217.1"
                     /translation="MRIILFLCFTLSYPLGYAKDKNICLLDDLLKNTTEYKNYLLSDE
                     ENTIELEKNELSLLPDIFINSSQNSNNDRSFRSVENSGLSIGFSQGIYSGGAYKKNK"
     gene            complement(300..1307)
                     /locus_tag="ACHJ69_004367"
     CDS             complement(300..1307)
                     /locus_tag="ACHJ69_004367"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFO5017218.1"
                     /translation="MTDSLIYSVSQRAESELKIQGDNVITIDFRTPVTIEKVNSFFSS
                     EPYSISFSKRSYLNFGGSPLSEEIHTITGVDSLRIKLWGIKTSKEFTGNVIIFPASKR
                     QPSSYINGIPFYSVATMNSRSTDFLDSLGLKNNNHSQNIVMPLETMFRFTIDNNIDNV
                     ELIKKTEVTNSDITSIMNILTIHGINDYNISSYLDAKETVRNVMNRFSILTNAVYIML
                     TIMALVVVHTIINRNFQLRNTEFAIKVIHGVNKNIMILVVVIETFIVTIASVCLSLFI
                     SYIMKFFLSRLLHAEIVFRFNMVLISLLLVIAVCNISGILSGLSFFKKNPLQILKERM
                     L"
     gene            complement(1610..2641)
                     /locus_tag="ACHJ69_004368"
     CDS             complement(1610..2641)
                     /locus_tag="ACHJ69_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFO5017219.1"
                     /translation="MPIIAPIPRTERRLMQKTIHKTKDKNHTRRLTAILMLHRGDTVC
                     HVARTLCCARSSIGRWINWFTLSGAEGMKSLPSGRGRRWPFEHICALLRELIKHSPGD
                     FGYQRSRWSTELLAIKIHEVTGCPLHASTIRRWLPAAGLVWRRATPTLRIRDPHKEEK
                     MVAIHEALAKCSAENPVFYEDEVDIHLNPKTGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSYVGGNSKGSSLFSSLLKHMKATYRRAKTITLIVDNYIIHKSRETLRWLK
                     ANPKFRVIYQPVYSPWVNHVERLWQALHETITRNHQCRSMWQLSKKVRHFMDTVSPFP
                     GGKHGLAKV"
     gene            2915..4081
                     /gene="sopA"
                     /locus_tag="ACHJ69_004369"
     CDS             2915..4081
                     /gene="sopA"
                     /locus_tag="ACHJ69_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HFO5017220.1"
                     /translation="MRLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQELALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            4081..5052
                     /locus_tag="ACHJ69_004370"
     CDS             4081..5052
                     /locus_tag="ACHJ69_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HFO5017221.1"
                     /translation="MKRAPVIPKHTIKTQPLEDTPSSAPAAPMVDSLIARVGAMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKSSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQRAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRYQFAPGATVLYKGDKMVLNLDRSRIPTECIERIEAILKELEKTAL"
     gene            5464..5793
                     /locus_tag="ACHJ69_004371"
     CDS             5464..5793
                     /locus_tag="ACHJ69_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5017222.1"
                     /translation="MSKPRWTIEQKRQHVAAWRASGLTRQQYCELNDIPFTSLREWPQ
                     DVAKAERRANEPTVLPVHIAPPLHADVPQPVTNEPVTLFLPGGVRMCCQPSQLTDVFR
                     ALKYAQS"
     gene            complement(5864..7477)
                     /locus_tag="ACHJ69_004372"
     CDS             complement(5864..7477)
                     /locus_tag="ACHJ69_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="HFO5017223.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     TRAEEKIEEDEREIEHLRAQIEKLRRMLFGPRSEKLRREVELAEALLKQREQDSDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGGELDYLGEVSAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFARQGVELSRALLSNWVDACCQLMTPVNDALYRYVMNTRKVHTDDTPVKVLA
                     PGQKKAKTGRIWTYVRDDRNVGSSSPPAVWFAYSPNRQGKHPEQHLRPFRGILQADAF
                     TGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(7508..7858)
                     /gene="tnpB"
                     /locus_tag="ACHJ69_004373"
     CDS             complement(7508..7858)
                     /gene="tnpB"
                     /locus_tag="ACHJ69_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5017224.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSSRNSLTML"
     gene            complement(7855..8280)
                     /locus_tag="ACHJ69_004374"
     CDS             complement(7855..8280)
                     /locus_tag="ACHJ69_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5017225.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTFSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
BASE COUNT         2170 a         1935 c         1891 g         2400 t
ORIGIN      
        1 tttatttttt ttatatgctc ctccactgta aattccttga gaaaagccaa tagaaagacc
       61 gctgttttca acagacctga atgatctatc attattgctg ttttgtgatg agttaatgaa
      121 tatatcaggt aatagtgata gttcattctt ttcaagttca atggtatttt cctcatcaga
      181 caaaaggtaa tttttatatt cagtagtatt ttttaataag tcatcaagta ggcaaatatt
      241 tttatccttt gcatagccaa gtggatacga caaagtaaaa caaagaaata atattatcct
      301 catagcattc tttcttttag tatttgtaaa ggattttttt tgaaaaatga caagccagaa
      361 agtataccgg atatattgca tacagctatg accagcaata aagaaattaa taccatatta
      421 aatctaaata caatttccgc atgtaatagt ctggataaaa aaaacttcat tatatatgaa
      481 ataaatagcg ataaacatac gcttgcgatg gttacaataa acgtttctat taccacgacc
      541 aatatcatta tgttcttatt tacaccatga ataactttaa tagcaaattc agtattccgt
      601 aattgaaaat tcctgttgat aattgtatgg acgacaacta atgccattat agtcagcatt
      661 atatatacag cattagtgag aatactaaag cgattcatga cattacggac cgtttccttg
      721 gcatctaaat atgaactgat attatagtca ttaataccat gtatcgttaa gatgttcatt
      781 atagatgtta tatcagaatt agtcacttca gtttttttaa ttaattctac attgtcgata
      841 ttattatcga ttgtaaacct aaacattgtc tctaaaggca tcacaatatt ttgtgaatga
      901 ttgttattct tgagtccaag actgtcaaga aaatcagtag atctgctatt cattgtcgca
      961 acactataaa aaggtattcc atttatatat gaggatggtt gccttttact cgccggaaaa
     1021 attattacat ttcctgtaaa ttctttactt gtttttatac cccaaagctt tattcttaaa
     1081 gaatcaactc ctgtgatcgt gtgaatttcc tcagaaagag gactccctcc gaaattaaga
     1141 tatgaccttt ttgaaaaact aatactatac ggttcggatg aaaaaaaact gttcactttc
     1201 tctattgtta caggagttct gaaatctatt gtaataacat tatctccttg aatttttaac
     1261 tccgactcag ctctttgcga tacgctatat attaatgagt ctgtcactat aataccagtg
     1321 aaactgataa tcaaaaaaat agtaaaaaca atgaagcttt ttctgttttc atacaaattc
     1381 aggaatgcat cacaaatgca aataatgaac atataaaaac acacctaaaa tattaattgt
     1441 tttttatgct actatatata tttgttgtca ttcgctatga aaatatcatt ttaagcactc
     1501 gaaaaaaata atctcattct atatttgtta ttttagccta atgtgctgta cttattccaa
     1561 gtatttcttt ttaacactcg catctactaa ataactgagc ctaatatcgc tacactttag
     1621 ccagcccatg tttccctccg gggaatgggc taacggtatc cataaagtga cgaacctttt
     1681 tcgacagttg ccacattgaa cggcactggt gatttcgggt aattgtctca tgaagcgcct
     1741 gccataatcg ttccacatga ttgacccacg gtgagtaaac cggctgataa atgaccctga
     1801 acttcgggtt tgctttcagc cagcgcaacg tttcacggct tttgtggatg atgtagttat
     1861 cgacaatgag cgtaatcgtt tttgcccgcc ggtatgtcgc cttcatatgt tttaacagac
     1921 tgctaaataa ggacgaacct ttactgtttc caccaacgta gctgactttt cccgtaccgc
     1981 tgtgcagcgc accggcgaga tagtactttt cattctggcc cggcgtgacc acgcgctttt
     2041 gctgcccacg tagctgccag tctgcgccag ttttgggatt gaggtgaata tccacttcat
     2101 cttcgtaaaa cacggggttt tccgcactgc atttcgccag tgcttcatgg atagcgacca
     2161 ttttttcttc tttgtgtggg tcacggatac gcagtgtcgg cgtggctctg cgccatacca
     2221 gtccggctgc aggtaaccac cgccggatgg ttgaagcatg tagtggacaa ccggtgacct
     2281 catggatttt tatcgccagc agttcagtac tccagcggga acgctgataa ccgaaatcac
     2341 cgggagaatg cttaataagc tcacgcagca atgcgcaaat atgttcgaac ggccagcgtc
     2401 tcccacgtcc tgacggtaat gacttcatgc cttcagcacc ggacagcgtg aaccagttaa
     2461 tccagcgacc aatcgatgag cgggcacagc agagcgttct ggcaacatga cagacggtgt
     2521 caccacgatg cagcatcagt atggcggtga gccggcgggt gtggtttttg tcctttgttt
     2581 tatggatagt tttctgcatc aggcgtcgtt cggttctggg tattggtgct atgatcggca
     2641 ttgctcagtc cggctggtga tttgggatat tcagctattg atcagatcgc ataaaccgga
     2701 ctgagttccc cctgagtgat ctactattcc gagcagttat ttagtgataa ttattgactg
     2761 aggtatgtgc ccttcctgtt tccttttttg atgtgttgct ctcattttaa acaactttgc
     2821 gtttttttga ctactttgca attttgttgt tgctttgcaa taaaatgcaa tattaaatga
     2881 aaatatgcaa agtaatgatt aaaggatgtt cagaatgaga ctcatggaaa cacttaacca
     2941 gtgtataaac gctggtcatg aaatgacgaa ggctatcgcc attgcacagt ttaatgatga
     3001 cagtccggaa gcgaggaaaa tcacccgacg ctggagaata ggtgaagcag cggatttagt
     3061 tggagtatct tctcaggcta tcagggatgc cgagaaagca ggccggctac cgcacccgga
     3121 tatggaaata cgaggacggg ttgagcaacg tgttggttat acaattgagc aaattaatca
     3181 tatgcgtgac gtgtttggta cgagactacg acgtgctgaa gacgtatttc cgccggtgat
     3241 tggagttgct gctcataaag ggggcgttta caaaacctct gtttctgttc atcttgctca
     3301 ggagctggct ctgaagggat tacgtgttct gctcgtggaa ggtaacgacc cccagggaac
     3361 agcatcgatg tatcacggct gggtgccaga tcttcatatt catgcagagg atactctcct
     3421 tcccttctat cttggggaaa aggacgatgt cacttatgca ataaagccta cttgctggcc
     3481 tgggcttgac attattcctt cctgtttggc tctgcaccgc attgaaactg agctaatggg
     3541 caaatttgat gaaggtaaat tgcccaccga tccacacctg atgctccgac tggccattga
     3601 aaccgtcgct catgactatg atgtcattgt cattgacagc gcgcctaacc taggtatcgg
     3661 cacgattaat gttgtatgtg ctgctgatgt gttgattgtc cccacgcctg ctgagttgtt
     3721 cgactacact tccgctctgc agtttttcga tatgcttcgt gatctgctca aaaacgtaga
     3781 tcttaaagga ttcgagcctg atgtacgtat tttgcttacc aaatacagta atagtaatgg
     3841 ttctcagtcc ccgtggatgg aggaacaaat tcgggacgcc tggggaagca tggtcctaaa
     3901 aaatgttgtg cgtgaaacgg atgaagttgg taaaggtcag atccggatga gaactgtttt
     3961 tgaacaggct attgatcaac gctcttcaac aggtgcttgg agaaatgccc tttctatttg
     4021 ggaacctgtc tgcaatgaaa ttttcgatcg tttgattaaa ccacgctggg agattagata
     4081 atgaagcgtg cgcctgtcat tccaaaacat acgattaaga ctcaaccgct tgaagatact
     4141 ccgtcatcgg caccagctgc accgatggtg gattcgttaa ttgcgcgcgt aggagctatg
     4201 gctcgtggca atgctattac tttgcctgta tgtggtaggg acgtgaaatt tacccttgag
     4261 gtgctccggg gagatagtgt tgagaaaagc tctcgggtat ggtcaggtaa tgaacgtgac
     4321 caggaactgc ttaccgaaga cgccctggat gatcttatcc cttcttttct actgacaggt
     4381 cagcagacac cagcattcgg tcgaagagtg tctggggtca tagaaattgc cgatgggagc
     4441 cgccgtcgta aagctgctgc acttacagaa agtgattatc gagttctggt tggtgagctg
     4501 gatgatgagc agatggctgc attgtccaga ttgggtaacg attataggcc aacaagtgct
     4561 tatgaacgtg gtcagcgtta tgcaagccga ttgcagaatg aatttgctgg aaatatttct
     4621 gcgctggctg atgcagaaaa tatttcacgt aagattatta ctcgctgtat caacacagct
     4681 aaactgccta aatcagttgt tgctcttttt tctcatcccg gtgaactatc tgcccggtca
     4741 ggtgatgcac ttcaaagagc ttttacagat aaagaagaat tacttaagca gcaggcatct
     4801 aaccttcacg agcagaaaaa agcaggagtg atatttgaag ctgaagaagt cattactctt
     4861 ttaacgtctg tgcttaaaac gtcatctgcc tcaagaacta gcttaagctc acgatatcag
     4921 tttgctcctg gagcgacagt gttgtacaag ggcgataaaa tggtgttaaa cctagataga
     4981 tctcgtattc caactgagtg tatagagaga attgaagcca ttcttaagga acttgaaaaa
     5041 acggcacttt gatgcggcac gttttagtct acatttatct gtctttactt aatttccttt
     5101 agcacaggct agaaaacata actaactggc ctgaatatta tatctgggac cacggtccca
     5161 ctcatattgt cggtctgata atcagtctgg gaccacggtc ccactcatat tgttggtctg
     5221 attatcgctc tgggaccacg gtcccactca tattgttagt ctgataatca gtctgggatc
     5281 gcggtccctc tcgtattgtt ggtctgataa tcagtctggg accacggtct cacttgtatt
     5341 gtcgatctga ttatcagagt gagactacga ttccatgtgt cgttacgggg aagtcatctt
     5401 cacagggatc ttcggtcagt tagtattacc gacatccatt caccaactta ccggagataa
     5461 tcgatgtcaa aaccccgatg gaccattgaa cagaaaaggc agcacgttgc tgcatggcgc
     5521 gccagtggtc ttacacgtca gcaatactgt gaactcaacg atattccctt cacatcactt
     5581 cgcgaatggc cgcaggatgt cgcaaaagct gaacgccggg caaatgaacc gaccgttctt
     5641 cccgttcata ttgcgccacc actgcatgct gatgtaccac agcccgtcac gaatgagcca
     5701 gtcacgctct tccttcccgg cggagtacgg atgtgctgtc agccgtcaca acttaccgat
     5761 gtcttcaggg cactcaaata tgctcagtcc tgataatgag gttgcgaata cggggtatga
     5821 gtctgtaacc aaagtaagcg tcaacggagc accgtattga cgcttattta ttggtgagta
     5881 ctacgttcca tggcaggagt tcatcaactc ggttggaagg ccattccggc agtacgctca
     5941 ggatatggcg cagatacgct tccggatcga taccgttcag acggcaggtg ccgatcagcc
     6001 cgtacaacag tgctccacgc tcgccgccgt gatcgctgcc aaagaacata aagtttttct
     6061 ttccgagaca gactgcacga agcgctcttt ccgcagcatt attatccgcc tccgccagac
     6121 cgtcatcact gtaataacag agggcatccc actgattcag tacatagctg aacgcttcgc
     6181 ccagtctgga ttttttcgac agcgtaccat tcttctccac catccattca tgcagcgacg
     6241 tcagtaacac tttgcttcgc tgctgcctga cggcaagacg ctctgactcc ggtaatcccc
     6301 gtatttcatc ctcgatggcg tacagttcac tgattcgctt cagggcttct tctgccgtcg
     6361 cacttttgct gctgatgtat acatcgtgga tttttcgccg ggcatgggcc cagcacgcaa
     6421 cttctgtcag tgcaccacct tcacgttctg cactgaacaa cctgtcgtaa cctgtgaacg
     6481 catccgcctg caggataccc cggaaggggc ggaggtgttg ctccgggtgt ttcccctgcc
     6541 ggttcggcga gtacgcgaac cagaccgctg gaggagatga cgaacccaca ttgcgatcat
     6601 cccggacata cgtccagata cgccctgttt tcgccttttt ctgacccggt gccagtacct
     6661 ttaccggtgt gtcatcagtg tgaaccttgc gggtgttcat tacataacgg tacagggcat
     6721 cattcaccgg tgtcattaac tggcagcacg cgtcaaccca gttggagagt aaggcccggc
     6781 tcagttcgac accctggcgg gcaaagattt cactctgacg atacagtggc agatgttcgc
     6841 agtattttcc cgttaacacg cgggcaagta atccggggcc cgcgatacca cgctctatcg
     6901 ggcgggacgg cgccggtgct tcaacaatac agtcacattt tgtacaggct ttttttaccc
     6961 gttctgtgcg gatcactttc agggcactgc tcaccagttc cagctgttca gcgctgactt
     7021 cccccagata atccagctca ccgccacact ccgggcaaca gctttcttct ggctccaggc
     7081 ggtgtatttc acggggaagg tgtgccggta acggacgacg atggcgcgac tgtcgcaact
     7141 ggcggggaac ctgaggatcg tcttcccgcc cactgtaacg atcgctgtcc tgttcacgtt
     7201 gtttcagcag agcctcagcc agttcaactt cacgacgcag tttttcagaa cggggaccga
     7261 acagcatccg gcgcagtttt tctatctgag cccgcagatg ttctatttcc cgttcatctt
     7321 cttcgatctt ttcttcggca cgtgtcagtg cagagcgcag gaaggcttcc gtctcttcaa
     7381 ccagactcag ttgctggtct ttctgacgga gggcttcagc ctgctcagag agcaaccttt
     7441 ccagctctgc gatgcgaatg aggtatttct gactcatgac cgtttttata atgcggtcag
     7501 gagtttttta caacattgtc agtgagttac ggctggatgt ttttggctga cgccagtcca
     7561 gcttatcgag gagcattgcc agttgcgagc gggtaatgga taccttgccg tcacgtaccg
     7621 caggccagat aaactggcct tcctccaggc gtttggtgaa caggcacaga ccatcagcat
     7681 cagcccaaag aattttgacg gtgtcacccc gtcggccacg gaagataaac aggtgaccgg
     7741 agaagggatt atcattcagc acatgttgta cctgttctcc cagtccgttg aaggatttac
     7801 gcatatcggt aacaccggca acgagccaga tacgggtacc tgatgggagt gagatcatct
     7861 tcccctcccg gtcagttcac ggatcaacac cgtgagcagc tctggcgatg gattttccag
     7921 cgtcatgtta ccgtgacgga attccacctt gcaggaactg gcactgactc tggtctgagt
     7981 ggaagtggat aaagacggcg caatggccgc cacaggttct ttctgctcat ccggcgttat
     8041 ttctacaggt aataattcaa cgccagtgtc agaaaaggtc gttaccggaa gacgccgcga
     8101 aacacgccct tcgttctgcc agagcctgag ccatttgaaa ataacattat cattgacgcc
     8161 attttcacgt gcaatctgtg caacacaagc tccaggttgt gatgccagtt ccaccatacg
     8221 aagtttgaat tcattcgaat agtttttacg aggttctttt cgccagtcct gtaattccat
     8281 acttagatgt ccgtctatat cagatgggcg tctaagttac caattctcgt ctgatggcta
     8341 catacggcgg tcagtttacg cttactaacc aaaagagtga aacattgaaa atgttg
//
LOCUS       DAZFFE010000154         8308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFFE010000154 DAZFFE010000000
VERSION     DAZFFE010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.155"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..675
                     /locus_tag="ACHJ69_004375"
     CDS             <1..675
                     /locus_tag="ACHJ69_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO5017226.1"
                     /translation="NYNRLFIPRQKTKDNNGLKTLNSKAVGRLLAAGGIYNGNIEGFR
                     ETANKLGGDAPAGYDQVMDNKGLLIAGASIVAGVVLGRMRFPDLESFEHFGARGAVSG
                     RPFNPDLAGGPIENLTIDGVTINREGIAIVEKHISRFDHDPANDVMISRLKKIANKEL
                     LPEKYDLNYYTHECREYQRYCNLGWETGEPNGLDGYELWNNAHTATLEDFKIKDTDLF
                     HPDAKK"
     gene            672..929
                     /locus_tag="ACHJ69_004376"
     CDS             672..929
                     /locus_tag="ACHJ69_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO5017227.1"
                     /translation="MTITIVEFNVLKVMAEKDIDWSWMVLDRTLAIRNIPGFGNVANI
                     VTKLVNHGLVDIVNGEGNSKPRYRVSQYGLNLIKEQQDNLF"
     gene            1004..1249
                     /locus_tag="ACHJ69_004377"
     CDS             1004..1249
                     /locus_tag="ACHJ69_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO5017228.1"
                     /translation="MITKNELNVLNVMTEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVSEKGYNLVQRENK"
     gene            complement(1418..3679)
                     /gene="metE"
                     /locus_tag="ACHJ69_004378"
     CDS             complement(1418..3679)
                     /gene="metE"
                     /locus_tag="ACHJ69_004378"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO5017229.1"
                     /translation="MTILNHTLGFPRVGLRRELKKVQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDTLNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            3797..4750
                     /gene="metR"
                     /locus_tag="ACHJ69_004379"
     CDS             3797..4750
                     /gene="metR"
                     /locus_tag="ACHJ69_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO5017230.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            complement(4638..5537)
                     /gene="bioP"
                     /locus_tag="ACHJ69_004380"
     CDS             complement(4638..5537)
                     /gene="bioP"
                     /locus_tag="ACHJ69_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO5017231.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWIGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALNE"
     gene            complement(5613..6413)
                     /gene="yigL"
                     /locus_tag="ACHJ69_004381"
     CDS             complement(5613..6413)
                     /gene="yigL"
                     /locus_tag="ACHJ69_004381"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO5017232.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPEII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFNYALYEPGLLEPEGVSKVFFTCDSHEKLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLQDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            complement(6421..7443)
                     /gene="pldB"
                     /locus_tag="ACHJ69_004382"
     CDS             complement(6421..7443)
                     /gene="pldB"
                     /locus_tag="ACHJ69_004382"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO5017233.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLGDP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEECVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7554..8174
                     /gene="rhtB"
                     /locus_tag="ACHJ69_004383"
     CDS             7554..8174
                     /gene="rhtB"
                     /locus_tag="ACHJ69_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO5017234.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            complement(8235..>8308)
                     /locus_tag="ACHJ69_004384"
     CDS             complement(8235..>8308)
                     /locus_tag="ACHJ69_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO5017235.1"
                     /translation="AKWIDGFAGALFAGFGIHLIISR"
BASE COUNT         2146 a         2171 c         2051 g         1940 t
ORIGIN      
        1 aactacaacc gtctgtttat accacggcaa aaaactaaag ataataatgg cctcaaaaca
       61 ctcaatagta aagcggttgg cagactgctt gctgctggcg gcatatacaa tgggaatatc
      121 gaaggattca gggaaacagc aaacaagttg ggaggagatg cacctgctgg ttacgatcaa
      181 gttatggata acaaagggtt actcatcgct ggtgcttcaa tagttgctgg agttgttctg
      241 ggaagaatga gattccctga tttggaatca tttgagcatt tcggagccag aggagcagta
      301 tcaggaagac catttaatcc cgaccttgcc ggtggtccaa tagaaaacct gacgatagat
      361 ggcgtaacaa tcaataggga aggtatcgct attgttgaaa agcatatctc cagatttgat
      421 catgatccag ctaatgatgt aatgatttca aggctaaaaa aaatagcaaa taaagaattg
      481 cttcctgaaa aatacgatct gaactactat actcatgaat gccgcgagta tcagcgttat
      541 tgtaatttag gatgggaaac aggtgaaccg aatggtttag atggatatga attatggaat
      601 aatgcacata ctgcaactct agaagacttt aaaattaaag atactgactt atttcatccg
      661 gatgcaaaaa aatgacgatt acgatagttg aatttaacgt gctgaaagtt atggctgaga
      721 aagatattga ctggagctgg atggtgctgg atcgtacttt agctatacga aatattccag
      781 gatttggcaa cgtagcgaat atcgtaacaa agctggttaa tcatgggttg gttgatatcg
      841 taaatggtga gggcaactct aaaccacgct acagagtttc tcaatatgga ttgaatctta
      901 tcaaagaaca acaagacaac ctcttctgat ccagccatta caggagcatg atttacattg
      961 tgatctttca ataagatact catcaatttt ggtacagaat atcgtgatca ctaaaaatga
     1021 acttaacgtg ctgaacgtta tgactgagaa agatataaac tggaactgga tgaatctcga
     1081 tcgcacacta tccataaggc aaataccagg attcggtaat gttgttaata ttgttaacaa
     1141 attagctaac gaaggtttgg ttattattga ggatagcggg cataagtcta tgcctcacta
     1201 ccacgttagt gaaaagggat acaatctcgt gcaacgtgag aataaataac gtagcgttga
     1261 ttactgaacg gaatcactca aaactgtgtt ttgcgaaaaa aacgattcgg ttatttctac
     1321 tcctgaagat ctgcttcaat ctggcactca tgcaaaatgg cttccagtac tggaagccat
     1381 tatcgtactg caatttacaa tgactcgtta atactcatca tccccgacgc aaattctgcg
     1441 ccgcctgcac catgttcgcc agtgccgcgc gggtttctgg ccagccgcgc gttttcaggc
     1501 cacagtctgg gttgacccac agacgctctg ccggaatgcg ttttgccgct ttcttcagca
     1561 aggcttcaat ccattccacg ctcggtacgt ttggcgagtg aatgtcatag acgccaggac
     1621 cgatttcatt tggataatca aactcttcaa acgactccag caactccatg tcggaacgcg
     1681 aggtttcgat ggtgatgacg tctgcgtcca gcgccgcaat cgaatccatg atgtcgttga
     1741 actcgcagta acacatgtga gtgtggattt gtgtgtcatc tttcgccacg gcggcgttga
     1801 tacggaaagc ctccacgccc cactggagat aggcatccca atcgctgcga cgcagcggta
     1861 aaccttcgcg caatgccggt tcgtcaatct gaatgatgcc aattccagcg gcttccaggt
     1921 ccgcgacttc atcacgcagc gccagcgcaa tttgtttggc gatggtttca cggctgacat
     1981 cttcacgcgg gaacgaccag cagagaatag tcaccgggcc ggtcaacatc cctttcaccg
     2041 gtttatcagt cagggattgc gcatattttg cccactccac ggtaatcggc gccggacgac
     2101 taacgtcacc aataacaatc ggtggcttca cgcagcggga accgtagctc tgtacccaac
     2161 cgttttgcgt aaagacaaac ccgtcgaggt gctcgccaaa gtattccacc atgtcattac
     2221 gctcggcctc gccatgtacc agcacatcca gtcccaaacg ttcctgctca acaatggcct
     2281 gcttgatatg ttccgcaatg cccgtacggt agttattggc gtcgagatta ccctttttga
     2341 aatccagacg cagggtacga atctccgtgg tttgcgggaa ggaaccaatc gtggtggtcg
     2401 gccacgcggg cagtttaaaa cgcgcacgct gggcttcagc acgcacttca tagacattcg
     2461 cacgctgact gtcctgagcg gtgatcgccg ccagacgctt ttctactgcc ggattatgta
     2521 cacgagtaga gtgacgacgc gcctgaatcg gagcgctcca ctctgccaga gctgccgtat
     2581 caccactgtt cagcgtatcg cgcagcaatg ccagttcatg acatttttgc agggcgaagg
     2641 caaaccagct tttcacttct gcatcaagac gcgtttccac gctcaggtcg atggggctgt
     2701 gcagcaacga gcaggaagat gccacccaca aatcgcgttt gccgacaatg tccttaattt
     2761 gcgcatattt ctcggtaaga tcggcgcgcc agacgttacg accattgata agacccgcag
     2821 acagcagcca gtcagaaggc agacgcttgt gcagttcagc aacgtcatct ttaccatgta
     2881 cgagatcgac atgcagaccc tgaacaggca gcgcagtaat cgtgtcgagg tttggcgtta
     2941 cgccttcaaa ataggtagtc agcagcagtt tcacctgccc ctgcagagca tcgtaagcgg
     3001 gtttgtacgc atccagccac gcctgcggca gttccagaac cagcgcgggt tcatcaatct
     3061 gtacccactc gatgccgcgt tttgccagtt ctgccagcac ttgctgataa accggcagaa
     3121 tgtcgttcag caggctcagg cgatcaaact gttcaccttt cactttaccc agccacaggt
     3181 aggtaaccgg ccccagcagc acgggcttga ccttgtggcc cagcgccagc gcctcgtcca
     3241 cttcctccag cagctgcgtc caggtcagtt tgaactgttg gcctttaacg aactccggca
     3301 ccatgtagtg atagttggtg ttaaaccatt tggtcatttc cgctgccgcc gcaggttcgc
     3361 cagtcggtgc acgtccacga ccaatacgga acagggtgtc gatatctacc gaaccatctt
     3421 tgttctgatg acgtggcgga acattaccca gcagcagact ggtggtcagt acatgatcgt
     3481 accaggcaaa atcgcccacc ggcagcaggt cgatacccgc ttgcttttgt tgatcccagt
     3541 gacgagcacg taattcacgc cctaccgcca gcagttcttc acgcgtggag ttccccgccc
     3601 aataactctc ttgcactttt ttcagctcgc gacgcaggcc aacgcgaggg aaaccgaggg
     3661 tgtgattaag aattgtcatt atatgcccct tatggaattt ttagtattta gccgtccaga
     3721 tgtttacaca tccataattg gcggttactg tatattcctc aagcgcaaat tattcatgcc
     3781 gaagtgaagg actttcatga tcgaagtaaa acacctgaaa acgctacaag cgttgcggaa
     3841 ctgcggctca ctcgcagccg ctgcggcgac gttgcatcaa acgcaatccg ccctgtctca
     3901 ccagtttagc gatctggaac aacgccttgg cttccggcta tttgtgcgta aaagccagcc
     3961 gcttcgcttt acaccgcagg gagaaatcct gttgcaactg gcaaatcagg tgctgccgca
     4021 aattagccag gcactgcaag cctgcaatga accgcagcag acgcgcctgc gcattgccat
     4081 agagtgccat agctgtattc agtggctgac gcccgcatta gaaaatttcc ataagaactg
     4141 gccgcaggtg gagatggatt ttaaatcggg cgtaacgttt gatccgcagc ctgcgttgca
     4201 acagggcgag ctggatctgg tgatgacgtc cgatattctg ccgcgcagtg gcctgcatta
     4261 ttcgccgatg ttcgactatg aagtacgtct ggtgttagca cctgaccatc cactggcggc
     4321 aaaaacgcgt attacaccgg aagatctcgc cagcgaaacg ctgttgattt atccggtgca
     4381 gcgtagccga ctggatgtct ggcggcattt tcttcagcca gcaggcgtca gcccgtcact
     4441 gaaaagcgtc gataacacct tattgttgat tcagatggtt gccgcacgga tgggtattgc
     4501 cgcactaccg cattgggtgg tggagagttt tgaacgccag ggtctggtgg tcaccaaaac
     4561 cctgggcgaa ggcttgtgga gccgactgta cgccgccgtg cgcgatggcg agcagcgtca
     4621 gccgattaca gaagcgttta ttcgttcagc gcgcaatcac gcctgcgatc atctgccgtt
     4681 tgtgaagagc gcggagcgac ccacttacga tgcacccaca gtgaggccag gatcaccagc
     4741 gcgcctgtaa taaacgttgg ccagtgcggc tgttggtgcc agatagccag gttaaccaac
     4801 agccctgccg gaacgtgcat attattcata atgcccagcg ttccggcgtc cacctgcgtc
     4861 gcgccgtagt tccacataaa gtagccaata ccggaagcca ccacgccaag aaacaccaga
     4921 atgccccatt gcagcgtggt ttgcggcatt ttctgcgcat ttcccagcaa gaaccatgca
     4981 atcactgcca ccaggaacgc gccaagatag aaccacgcaa aggcgttatg ctgcggcatc
     5041 gggcgagttt ccatcagacg tttgtaaccc accatgccga tggcaaaagt gatattggag
     5101 agttgcacca gcaataagcc aatccagaaa tggtcggtga cctgatcata gcgaataatc
     5161 ccggcaccaa tcaccgccag caaggcgcta aaggcatagc cccagcgcag acggcgctta
     5221 ctcatgatgt cataaatcag cgtgatgtag agcggcgtca gtacggtgaa caacagcagt
     5281 tcagaaaccg tcaggtagag ataggcgcgg aaactcagca tatacatcac accgagctgc
     5341 atcgcgccca ccagcatata caggccgacc gttttcaggc tattgccacg ggtacgcaga
     5401 aacggcagaa aaacgagtgc cgccaggcca acgcgcacca gcaccgcaaa atagctatcg
     5461 acgtgccccg caaggtactc gccataaaag ctaaaggaga aggcccacag aatcgtggtg
     5521 atgataagta gcgccacaat ggaaaactct ttgattaacg agtatccatt gtagcgaagt
     5581 atcaggttga caactgacca aataaagaac gattacgata aatagagctt acgcagataa
     5641 tgcggcactg cgtcttcggc gttagtacca atcacttcca gctctggatg cagatctttc
     5701 agccgttgat gcgcgctgcc cataatgcag cctttccccg ccatcgacag catttcggca
     5761 tcgttcattc catcgccaaa ggcaatacaa tcttgcaggc tgtagcccag tttcttcgcc
     5821 accgcttcca gcgcatggcc ttttgaaacg ccgcccgcca tcacttccag gcaggttaag
     5881 gtagagaaac tgacattgac gcgatctccc cagcgagcgt taatcgcctg ctccagtggc
     5941 agaagttttt catgggaatc gcaggtgaag aacactttgc tgacgccttc cggctccagc
     6001 aatcccggct catacagcgc ataattgaac accgcttctt tgaaaaagcg catctcttcc
     6061 gggcgatggc gattcataaa ccattcatca tcgcgataaa cgttagtaat gatctccggg
     6121 ttgtcgttga ccacgccaaa caggtcactg gcaatgtcgc gatccaggtt atgagcaaaa
     6181 atcaggttgc catccagatc gtgaacgcgc gcgccattgg aggtaatcat gtaagactta
     6241 atctccagat tatcgcgaat ttgccccaca tcaacgtggt gacgaccggt cgcaaacaca
     6301 aagttgatgc cgcgcgcggt gagcagcttc agagtttctt tggcgtaagg ggataacgta
     6361 tggtcgggag aaagtaacgt gccatctaaa tcagacgcaa caacctggta cataagaaat
     6421 ttaaacctct gtagagcggt ttccgctggg tgagttatgc ctgttgaaaa aatcaacgat
     6481 agcgtggagc gcgactgagc gcattgcgtc cttttcaaaa aggatctcat ggtacgcacc
     6541 tttaattacc aacggccgtc ctccttcgac aggatggccc gcagcggtgc gtagttcaca
     6601 aaaacggtcg tgcatgcggt tatccaccac gcattcctct tcagcttgta agagaagcgt
     6661 tggcgtggcg tcatcacccg caccggctaa cacctgttcg ccagccagaa tactttcgcg
     6721 tacccaatgg taggtcggcc caccgacgcg aatcgttggg tcatcagcat agaaacgtaa
     6781 gttacgtcga tatcgctgtc tgctgtgggt cagtacgttg atagcaaacg gcaacgcgcg
     6841 ccagcgcccg gtgcctattg catagccatc acggaaacgt ggatgcgctt cggcccaatt
     6901 gaggatctgc cgtgccataa atgacggcat acgaatcacg atcccaaaca ttggcgcagt
     6961 tagcgcaatg gcgtcacata cacctggatg gcgttgcaga aataatgtgg agatcgcacc
     7021 gcccatcgaa tgtgccagta tatagcgttt acgccacgga ccgggctgaa cctcctgttg
     7081 ccagaatgcc gccagatcat caacataatc attaaagcga ttaacatgcc cgagatgtgg
     7141 atcgcctaac aggcgaccgg aacgtccctg cccgcgatgg tcgatgatta aaacatcaaa
     7201 ccccaaatgg aacaggtcgt aggccagttc cgcatatttt acgtagctct caatacgccc
     7261 cggacagatg actaccaccc ggtcatggtg ctgtgcgcga aaacggacaa agcgcaccgg
     7321 aatgtcatcc acaccagtaa actctgcttc atcacgctga cgccagaaat cagtcagcgg
     7381 tcccatggta aaagcagcaa acgcgttttc tcttgtttcc cagtcttttt gctgctgaaa
     7441 catcgggtaa tctgcctctt aaaccacgta aaatcgtttt tttaagcgtg cctgacacaa
     7501 cgctgcgaca gtagcgtatt gtggcacaaa aatagacaca ccgggagttc atcatgacct
     7561 tagaatggtg gtttgcctac ctgctgacat cgatcatttt aagcctgtcg ccaggctctg
     7621 gcgcaatcaa caccatgacc acctcgctca accacggtta tcgcggcgcg gtggcgtcta
     7681 ttgctgggct tcagaccgga ctggcgattc atattgtgct ggttggcgtg gggttgggga
     7741 cgctattttc ccgctcagtg attgcgtttg aagtgttgaa gtgggcaggc gcagcttact
     7801 tgatttggct gggaatacag caatggcgcg ccgctggcgc aattgatctt aaatcgctgg
     7861 cctccactca atcgcgtcga catttgttcc agcgcgcagt ttttgtcaat ctcaccaatc
     7921 ccaaaagtat tgtgtttctg gcggcactat ttccgcaatt catcatgccg caacagccgc
     7981 agctgatgca gtatatcgtg ctcggcgtca ccactattgt ggtcgatatt attgtgatga
     8041 tcggttacgc cacccttgct caacggattg ctctatggat taaaggacca aagcagatga
     8101 aggcgctgaa taagattttc ggctcgttat ttatgctggt gggagcgctg ttagcatcgg
     8161 cgaggcatgc gtgaaaataa tgtcggatgc ggcgtaaacg ccttatccga cttactctga
     8221 tgacgcgtct ggcattaacg cgaaataatc aaatgaatgc caaatccggc aaataacgcc
     8281 ccggcaaaac catcaatcca cttcgcca
//
LOCUS       DAZFFE010000155         8246 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFFE010000155 DAZFFE010000000
VERSION     DAZFFE010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.156"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(15..674)
                     /locus_tag="ACHJ69_004385"
                     /pseudo
     CDS             complement(15..674)
                     /locus_tag="ACHJ69_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            968..1723
                     /gene="mlaA"
                     /locus_tag="ACHJ69_004386"
     CDS             968..1723
                     /gene="mlaA"
                     /locus_tag="ACHJ69_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO5017236.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADSLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(1785..3125)
                     /gene="fadL"
                     /locus_tag="ACHJ69_004387"
     CDS             complement(1785..3125)
                     /gene="fadL"
                     /locus_tag="ACHJ69_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO5017237.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNITGHSDLTGQSANAKNIAPT
                     AWVPNLHFVAPINDQFGWGASITSNYGLATEFTDNYAAGIYGGKTDLQTINLNLSGAY
                     RLNNSWSFGLGFDAVYAKAKIERYAGSLGPLLSQQLIAQGVPASLTNGINTPDSQIAH
                     LKGDEWGFGWNAGILYELDKDNRWGLTYRSEVKIDFDGDYKSSINPNLNNILGNMGLP
                     YGTMGATQNGSLTLNLPEMWEISGYNRVAPQWAIHYSMAYTSWSQFQELKAKGDSGQT
                     LFYKDEGFRDAYRIALGTTYYYDKNWTFRTGIAYDDSPVPADKRSISIPDQDRLWLSA
                     GLTYAFNEDASIDVGASYMHGQKVKFTEGEGAAAYSFESEGKAWLFGTNFNYAF"
     gene            3497..3781
                     /gene="yfcZ"
                     /locus_tag="ACHJ69_004388"
     CDS             3497..3781
                     /gene="yfcZ"
                     /locus_tag="ACHJ69_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO5017238.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            3962..5272
                     /gene="fadI"
                     /locus_tag="ACHJ69_004389"
     CDS             3962..5272
                     /gene="fadI"
                     /locus_tag="ACHJ69_004389"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO5017239.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARTEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     EGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            5272..7416
                     /gene="fadJ"
                     /locus_tag="ACHJ69_004390"
     CDS             5272..7416
                     /gene="fadJ"
                     /locus_tag="ACHJ69_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO5017240.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QIRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEVLARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQAVKLGLVDDVVPHSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARTFGEL
                     AMTPQSQALRNIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPRGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYCGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCATHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHASTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERIEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIMPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCDRLVEMSERGESFWKTTATDLQ"
     gene            7619..8104
                     /gene="sixA"
                     /locus_tag="ACHJ69_004391"
     CDS             7619..8104
                     /gene="sixA"
                     /locus_tag="ACHJ69_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO5017241.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVGAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGQFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2015 a         1985 c         2142 g         2104 t
ORIGIN      
        1 agggcaataa gctaggtcat caatataatc accacaatct ttgccgcacc cgctgcagga
       61 aacatccata ccatagtggc gatcagccag ccggaaatga tcgcgttggc aaacatctcg
      121 ctgggggtgt tcttcatcac atccatgccg attttggcaa atgcatcgcg agtttcttca
      181 ttgaagatag gcatatattc aaatgcccac gcagcaatac ctgtcccgat aatattaccc
      241 agcagcacga tgccccataa ccgcataagt aagccgacgt tgctcattgt cggtttttgc
      301 atgacgggta gtaccgcagt tacggtgttt tcggtaaata attgctggcg ggccattatg
      361 acgataataa aaccaaaggt ataaccgaga ttctccagca agaagctgcc cggcacgcct
      421 tccagttcga catgaaatat cccttttgcc agtagcgaag cgcccatcga caggcccgcc
      481 gcaatggctg accacagtag cgccattgcg tcgcgttcca gctctttttc accatcctgg
      541 cggatatgct catgaattgc catcgcccgg gaggggagtc ggtcttcatc tatttctatt
      601 tttttaccgc gctctttttc ttcgctctca acttcaattt cgtcgctgtg ttgatcaatt
      661 ttgtcgttgt ccatggtctc ttctcttgaa ttagcacgta tagctaaagc gtagcggctt
      721 ttttgctcgc aactggcggg agttactctg aaaatgtaga aaaggccgcg tttgcttttt
      781 tctgtttcta ttgaatcaag tagcctacag ggcggcgatt accaggctat gatcaaatca
      841 gcaaatcagg gcgtctggac atcagttgac gtgctgttac aatcgcccac acctaaacag
      901 gcggatacgg tatcgttccg tcatggatgg caaactgcat aagccataaa aaaacaggga
      961 gacatttatg aagcttcgcc tgtcggcgct tgctctggga actacgcttc tggtggggtg
     1021 tgcgagttcc ggtacagatc agcaagggcg ttctgacccg ttagaagggt ttaaccgcac
     1081 tatgtacaac ttcaacttca atgtattaga cccgtatatt gttcgaccgg tcgctgtcgc
     1141 ctggcgtgat tatgttccgc aacctgcgcg taacggtttg agcaacttta ccggcaacct
     1201 tgaagaacct gcggtgatgg ttaactactt cttgcagggc gacccttatc aggggatggt
     1261 tcactttacc cgctttttcc tgaataccat tttgggaatg ggcggcttta ttgatgttgc
     1321 agggatggcg aacccgaaac tgcaacggac tgaacctcac cgcttcggta gtacgcttgg
     1381 tcattatggt gtgggttatg ggccgtacgt tcagttaccg ttctacggta gcttcacgct
     1441 gcgtgatgac ggtggtgata tggcggatag tctttacccg gttctttcct ggctgacctg
     1501 gccgatgtct gtgggtaaat ggacgcttga agggatagaa actcgtgcgc agttgttgga
     1561 ttccgacggt ctgctgcgtc agtcctccga tccttatatt atggtgcgtg aagcgtactt
     1621 ccagcgtcat gatttcatcg ctaatggcgg tgaactcaaa ccgcaggaaa atccgaacgc
     1681 gcaagcgatt caggatgatt taaaagatat cgattctgaa taagaaacaa acaaaaaagg
     1741 tgagtcgcaa aactcacctt ttttcttatt aagctaacgc gttgtcagaa cgcgtagtta
     1801 aagttagtac cgaacagcca ggctttacct tcagactcga aggaataagc tgccgctcct
     1861 tcaccttcgg tgaatttcac tttctgacca tgcatataag atgcgccaac atcaatggat
     1921 gcatcttcat taaatgcgta ggttaaccca gcacttaacc acaaacgatc ctggtcagga
     1981 atggaaatag aacgtttatc tgccggaacc gggctgtcgt cataagcgat accggtacgg
     2041 aaggtccagt ttttatcata atagtaggtt gtacccaacg cgatacgata cgcgtcccgg
     2101 aatccttcat ctttatagaa cagagtctga ccactatcgc ctttcgcttt caattcctgg
     2161 aactggctcc aactggtata ggccatgctg tagtgaattg cccactgcgg cgcaacacgg
     2221 ttataaccag aaatctccca catctcaggc aggttcagcg ttaaggatcc attttgggtt
     2281 gcccccatcg tgccatacgg taagcccatg ttacccaaaa tattgttcag atttgggtta
     2341 atggagcttt tgtaatcccc atcgaagtcg attttaactt ctgaacgata tgtcagcccc
     2401 caacggttat ctttatccag ttcataaaga atacccgcgt tccagccaaa gccccattcg
     2461 tcacctttca aatgagcaat ttgagagtcc ggagtattaa taccattggt taatgaagcg
     2521 ggtacaccct gggcaatcaa ctgttgagac aacaatggcc ccagcgaacc ggcgtaacgc
     2581 tcaatttttg ccttggcata aactgcatca aaaccaagac caaaactcca gctattgtta
     2641 aggcgataag ccccgctaag gttaaggttg attgtttgca gatcggtttt accgccatag
     2701 ataccagcag cgtagttgtc ggtaaattcc gtagccaggc catagttgga agttattgac
     2761 gctccccaac caaactgatc gttaataggc gcaacaaagt gcaggttagg tacccatgct
     2821 gtcggtgcga tatttttagc atttgcactc tgaccggtta aatcagaatg tccggtaata
     2881 tttacatccg gatcaatata aaccgcacct gcagaaaatg tcgggcggtc aaacatggta
     2941 atcaatgcgg ggttacggct aacgttacct gcatcatcgg caattgcacc ttcccctgaa
     3001 taagcccggc ccaggccaga ggaagaaaat tcgtttaact gaaagcctgc cgaccaggcc
     3061 tgggtggaga taagtgccac tgcgactgcg agagcagact ttgtaaacag ggttttctgg
     3121 ctcatgacca taacctcaat gatttatttt tatacaaact atgttacgtg ctgtaacagg
     3181 agcgcgaagt gtagggtctg aggtacatct tagaaatcag accagtggcg agagtatagg
     3241 tcggaccagc tggaatgttg caagtatgtt tctatctttt ttcagatatg atctaagaaa
     3301 cgcgatccag ctggcaaaat tgggaggtgg cgcactcatg gattcgggtg aattttgttt
     3361 tagatcattt ttaagtgtga tttcggtcac ttatccgatt ttgcagaatt aacaactgga
     3421 gcagcacttt ccggaagcgt aaaatatcca caacgtttat ctggcacctt gggtgcacat
     3481 acagaggaaa tcaatgatga gtaaatgtag tgctgatgaa accccggttt gctgctgtat
     3541 ggatgttggc accattatgg acaactccga ctgcaccgcg tcttacagcc gtgtgtttgc
     3601 aaaccgcgca gaagcagaac aaacgctggc ggcgctgact gaaaaagccc gtagcgtgga
     3661 atccgaaccg tgcaaaatca ccccgacttt cactgaagag tctgacggtg tgcgcctgga
     3721 tatcgatttc acttttgcct gcgaagctga aatgctgatc ttccagctgg gtctgcgtta
     3781 attcctctcg ggtacatacg cccctgtcag ttgctggcag gggcgttttc ttttctcttc
     3841 ttcttttctc tctgtgtcat agctcccact ttcccttttc tccacttggc taaatgtaaa
     3901 aaaatggtta agactatgat caggtcagac cactttattt atttttttac aggggagtgt
     3961 tatgggtcag gttttaccgc tggttacccg ccagggcgat cgtatcgcca ttgttagcgg
     4021 tttacgtacg ccttttgccc gacaggcgac ggcttttcat ggcattcccg cggttgattt
     4081 agggaagatg gtggtaggcg aactgctggc acgcaccgag atccccgctg aagtgattga
     4141 acaactggtc ttcggtcagg tcgttcagat gcctgaagcc cccaacattg cccgtgaaat
     4201 tgttctcggt actggaatga atgtgcatac cgatgcttac agcgttagcc gcgcttgcgc
     4261 taccagtttc caggcggttg caaacgtcgc agaaagcctg atggcgggaa ctattcgagc
     4321 ggggattgcc ggtggggcag attcctcttc cgtattgcca attggcgtca gtaaaaaact
     4381 ggcgcgcgta ctggttgatg tcaacaaagc ccgcacaatg agccagcgcc tgaaactttt
     4441 ttctcgctta cgtttgcgtg acttaatgcc ggtgcctccg gcagtggcgg aatactccac
     4501 tggattacgg atgggggaca ccgcagagca aatggcgaaa acctacggca tcacccgaga
     4561 acagcaagat gcactggcgc accgttcgca tcagcgtgcc gctcaggcgt ggtcagaagg
     4621 taaactcaaa gaagaggtga tgactgcctt tatcccccct tataaacaac cgcttgtcga
     4681 agacaacaat attcgcggta attcctctct tgctgattac gcaaagctgc gtccggcgtt
     4741 tgatcgtaaa cacgggacgg taacggcggc gaacagtacg ccgctgaccg atggcgcggc
     4801 agcggtgatc ctgatgactg aatcccgggc gaaagaatta gggctggtac cgctggggta
     4861 tctgcgcagc tacgcattta ctgcgataga tgtctggcag gacatgttgc tcggtccagc
     4921 ctggtcaaca ccgctggcgc tggaacgtgc cggattgacg atgagcgatc tgacattgat
     4981 cgatatgcac gaagcctttg ccgcccagac gctggccaat attcagttgc ttggcagcga
     5041 acgttttgct cgcgacgtac tggggcgtgc acatgccact ggcgaagtgg acgatagcaa
     5101 atttaacgtg cttggcggtt cgattgctta tggacatccc ttcgcagcga ccggcgcgcg
     5161 gatgattacc cagacattgc atgaacttcg ccgtcgcggc ggtggatttg gtttagtgac
     5221 cgcctgtgct gcgggtgggc ttggcgcagc aatggttctg gaggcggaat aatggaaatg
     5281 gcatcagcgt ttactcttaa tgttcgtctg gacaacattg ctatcatcac catcgacgta
     5341 ccgggtgaga aaatgaatac cctcaaggcg gaatttgcct cgcaggtacg cgccattatt
     5401 aagcaaatcc gtgaaaacaa agagttgcgt ggcgtggtgt ttgtctccgc gaaaccagac
     5461 aacttcattg ctggcgcaga catcaacatg atcggcaact gcaaaacggc gcaagaagcg
     5521 gaagtactgg cgcggcaggg gcaacagtta atggcggaga ttcatgcttt gcccattccg
     5581 gttatcgcgg ctattcatgg cgcttgcctg ggcggcgggc tggagttggc gctggcgtgc
     5641 cacggtcgtg tttgtactga cgatcctaaa acggtgctcg gtttgcctga agtacaactt
     5701 ggattgttac ccggttcagg cggtacccag cgtttaccgc gcctgatagg tgtcagcaca
     5761 gcattagaga tgatcctcac cggaaaacaa cttcgggcga aacaggcggt aaagttgggg
     5821 ttggtggatg acgtcgttcc gcactccata ttgctggaag ccgctgttga gctggcaaag
     5881 caggatcgcc catcttcacg ccctctacct gtacgcgagc gtattctggc ggggccgtta
     5941 ggtcgtgcgc tgctgttcaa aatggtcggt aagaaaacag aacacaaaac gcaaggtaac
     6001 tatccggcga cagaacgcat cctggaggtt gtcgaaacgg gattagcgca gggcaccagc
     6061 agcggctatg acgctgaagc acggacgttt ggcgaactgg cgatgacgcc gcaatcgcag
     6121 gcgctgcgca atatcttttt tgccagtacg gacgtgaaga aagatcccgg cagtgatgcg
     6181 ccgcctgcgc cattaaacag cgtggggatt ctaggtggtg gcttgatggg cggcggtatt
     6241 gcttatgtca ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat caacccgcgg
     6301 ggcataaatc atgcgctgaa gtacagttgg gatcagcttg agggcaaagt tcgccgtcgt
     6361 catctcaaag ccagcgaacg tgacaaacag ctggcattaa tctccggaac gacggactat
     6421 tgcggctttg cccatcgcga tctgattatt gaagcggtgt ttgaaaatct cgaattaaaa
     6481 caacagatgg tggcggaagt ggagcaaaat tgcgctactc ataccatctt tgcttcgaac
     6541 acgtcatctt taccgattgg cgatatcgcc gctcacgctg cgcgacctga gcaagttatt
     6601 ggcctgcatt tcttcagtcc ggtggaaaaa atgccgctgg tggagattat tcctcatgcg
     6661 agtacatcgg cgcaaaccat cgccaccacg gtaaaactgg cgaaaaaaca gggcaaaacg
     6721 ccaattgtcg tgcgtgacaa agccgggttt tacgtcaatc gcatcttagc gccttacatc
     6781 aatgaagcta tccgcatgtt gaccgaaggc gagcgtattg agcatatcga tgccgcgctg
     6841 gtgaaatttg gttttccggt aggcccaatc caacttttgg atgaggtagg aatcgacacc
     6901 gggactaaaa ttatgcctgt actggaagcc gcttatggag aacgttttag cgcgcctgca
     6961 aatgttgttt cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg ccggggtttc
     7021 tatctttatg gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcccgc catttacccg
     7081 ctcattggcg cacaagggca ggggcgactc tccgcaccgc aggttgctga acggtgtgtg
     7141 atgttgatgc tgaatgaagc agtacgttgt ctggacgagc aggttatccg tagcgtacgt
     7201 gacggggata ttggcgcggt atttggcatt ggttttccgc catttctcgg tggaccgttc
     7261 cgctatatcg attctctcgg cgcaggcgaa gtggttgcaa taatgcaacg actggccacg
     7321 cagtatggtt cccgttttac cccttgcgat cgtttagttg agatgagcga gcgtggggaa
     7381 agtttttgga aaacaactgc aactgacctg caataagaag gtcaaagtta tatgaatccg
     7441 cgctgaatgg cggagttttg ctcaaaatgt aaacgcatat tgactatact tacgccattg
     7501 aggtagaaaa cagcgtttca ttcggtgaat ggataaggca caatgccggc caccgttttc
     7561 tttctctggt ttcagatgaa agaaaacggg cgaatctggt taacaaaagc ggtgcaatat
     7621 gcaagttttt atcatgcgtc acggcgacgc agccctcgat gccgccagtg attcggttcg
     7681 tcctctgacc actaacggtt gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca
     7741 aaaagtggaa atcgaacgcg ttctggtgag cccgttcctg cgagccgagc aaacactgga
     7801 agaagtaggg gattgtctga acctgccttc cagtgcagaa gttctgccgg agttaacgcc
     7861 ctgcggcgat gttggtctgg tcggcgccta tttgcaggcg ttgaccaatg aaggtgttgc
     7921 ttcggtactg gtgatctccc acttaccgtt agtcgggtat ctggttgccg agttatgtcc
     7981 gggcgaaacg ccgcctatgt ttaccacttc agcgattgcc agcgtaacgc ttgatgaaag
     8041 cggaaaaggc caattcaact ggcaaatgag cccttgtaat ttgaagatgg caaaagcaat
     8101 ttaagcaatc aatgcccggt tacgtgcgcg tacaccgggc agtttctccg tcatacaatc
     8161 attctttttc ttgtctaaat gttgttaata tttaacaact ctcgatagtt attatatttt
     8221 cccattattg aatcccgtaa taaaat
//
LOCUS       DAZFFE010000156         8059 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFFE010000156 DAZFFE010000000
VERSION     DAZFFE010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.157"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..80
                     /locus_tag="ACHJ69_004392"
     CDS             <1..80
                     /locus_tag="ACHJ69_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO5017242.1"
                     /translation="SLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            97..2601
                     /locus_tag="ACHJ69_004393"
                     /pseudo
     CDS             97..2601
                     /locus_tag="ACHJ69_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            2660..4720
                     /locus_tag="ACHJ69_004394"
     CDS             2660..4720
                     /locus_tag="ACHJ69_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO5017243.1"
                     /translation="MAVQISGVLKDGAGKPIQNCTIQLKAKRNSTTVLVNTVASENPD
                     EAGRYSMDVEYGQYSVTLLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVMPEA
                     LRRFEVMVEDVARNAEAASQSAAAAKKSETAAASSRNAAKTSETNAGNSAKAAASSKT
                     AAQNAATAAERSETNARASEEASADSEEASRRNAESAAENAGVATTKAREAAADATKA
                     GQKKDEALSAATRAEKAADRAEAAAEVTAEPCANIVPPLPDVWIPFNDSLDMIAGFSP
                     GYKKIAIGDDVVQVASDKQVNFSRASTATYINKSGELKTAEINEPRFECDGLLIEGQR
                     TNYMLNSESPASWGKSSNMDVPETGTDSFGFTYGKFVCNDSLVGQTSAINMASIAATK
                     SVDVSGDNKYVTTSCRFKTERQVRLRIRFDKYDGSATTFLGDAYIDTQTLEINMTGGA
                     AGRITARVRKDKTTGWIFAEATIQAIDGELKIGSQIQYSPEQGGATVSGDYIYLATPQ
                     VENGPCVSSFIISGGSATTRASDLVSIPTRNNLYKLPFTFLLEIHKNWDIAPNAAPRV
                     WDIAAANTGQSAIAAINRGSGKLYMSLSNPSGSYVNSAATDVFAEKTTFGCIAKADGH
                     FHVVTNGKAVNEVYCEYNGVTADKNIRFGGQTNTGERHLFGHIRNFRIWHKELNDRQL
                     KEVV"
     gene            4717..4995
                     /locus_tag="ACHJ69_004395"
     CDS             4717..4995
                     /locus_tag="ACHJ69_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017244.1"
                     /translation="MKDLTLKFHDKLQFKAFLSSLGWAEDEDLQNKLLVDEIGFTYTE
                     TGVTEEGEPVCIRNDGYFVNIRILDDLFDVSVFSDYVVELETPLREWS"
     gene            5008..5298
                     /locus_tag="ACHJ69_004396"
                     /pseudo
     CDS             5008..5298
                     /locus_tag="ACHJ69_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5393..6250)
                     /gene="sitD"
                     /locus_tag="ACHJ69_004397"
     CDS             complement(5393..6250)
                     /gene="sitD"
                     /locus_tag="ACHJ69_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFO5017245.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTMIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISILVSMTGVWLSFFIDSAPAPTIVVLFAVLFIMTFAVTSINARKKGNS
                     HTQDLLSPN"
     gene            complement(6247..7104)
                     /gene="sitC"
                     /locus_tag="ACHJ69_004398"
     CDS             complement(6247..7104)
                     /gene="sitC"
                     /locus_tag="ACHJ69_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFO5017246.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKLLFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(7101..7928)
                     /gene="sitB"
                     /locus_tag="ACHJ69_004399"
     CDS             complement(7101..7928)
                     /gene="sitB"
                     /locus_tag="ACHJ69_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFO5017247.1"
                     /translation="MMQSAGIVVNDVAVTWHNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVNFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            complement(7928..>8059)
                     /locus_tag="ACHJ69_004400"
     CDS             complement(7928..>8059)
                     /locus_tag="ACHJ69_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFO5017248.1"
                     /translation="HYGGVLYVDSLSTENGPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         2149 a         1928 c         2202 g         1780 t
ORIGIN      
        1 agtcgctgtc gttctcaaaa tcggtggagc tgcatgacaa agtcatcggg cattatctga
       61 acataaaaca ctatcaataa gttggagtca ttaccatggg cgctgtatgt catcggccag
      121 aattgcgacc agtcggtgcc ggatggtttt ggtggcacgg agccgcgcat cacctgtaat
      181 gcctggctga ccacacagcg taaggcgtgg gatgttctca gtgatttctg ctcggcgatg
      241 cgctgtatgc cggtatggaa cgggcagacg ctgacgttcg tgcaggaccg gccgtcggat
      301 aaggtgtgga cctataaccg cagtaatgtg gtgatgccgg atgatggcgc gccgttccgc
      361 tacagtttca gcgccctgaa ggaccgccat aatgccgttg aggtgaactg gattgacccg
      421 gataacggct gggagacggc gacagagctt gtggaggaca cgcaggccat tgtccgttac
      481 ggtcgtaacg tcacgaagat ggatgccttt ggctgtacca gtcgggggca ggcacaccga
      541 gccgggctgt ggctgattaa aacggaactg ctggaaacgc agaccgtgga tttcagcgtg
      601 ggcgcagaag ggcttcgcca tgtgccgggc gatgttattg aaatctgcga tgatgactat
      661 gcgggtatca gcatcggcgg gcgcgtgctg gcggtgaaca gccagacccg gacgctgacg
      721 ctcgaccgtg aaatcactct gccatcctcc ggtaccacgc tgataagcct ggttgacgga
      781 agcggcaatc cggtcagcgt ggaggtccag tccgtcaccg acggcgtgaa ggtaaaagtg
      841 agccgggttc ctgacggcgt tgccggatac agcgtgtggg ggctgaagtt gccgacgttg
      901 cgccagcgcc tgttccgctg cgtgagtatc cgtgagaacg atgacggcac gtatgccatc
      961 actgccgtgc agcatgtacc ggaaaaagag gccatcgtgg ataacggggc gcactttgac
     1021 ggcgaccaga gcggcacggt gaatggtgtc acgccgccag cggtgcagca cctgactgcc
     1081 gaagtcaccg cagacagcgg ggaatatcag gtgctggcgc gctgggacac gccgaaggtg
     1141 gtgaaggggg tgagctttat gcttcgcctg accgtggcag cggatgacgg cagtgagcgg
     1201 ctggtcagca cggcccggac gacggaaacc acataccgct tcacgcaact ggcgctgggg
     1261 cggtacacgc tgacagtccg ggcggtaaat gcgtgggggc agcagggcga tccggcgtcg
     1321 gtatcgttcc ggattgccgc accggcagca ccgtcgagga ttgatctgac gccgggctat
     1381 tttcagataa ccgccacgcc gcatcttgcc gtttatgacc cgacggtaca gtttgaattc
     1441 tggttctcgg aaaagcggat taccgatatc aggcaggttg aaaccacagc ccgctatctt
     1501 ggcacggcgc tgtactggat agctgccagt atcaatatta agccgggcca tgattattat
     1561 ttttatatcc gcagtgtgaa tactgttggc aaatcggcat tcgtggaggc tgtcggtcag
     1621 gcgagcgatg atgcttccgg ctatctggat tttttcaaag gccagataac tgaatcccat
     1681 ctcggcaagg agctgctgga aaaagtcgag ctgacggagg ataacgccag caaactggag
     1741 gagttttcga aagagtggaa agacgccaac gataaatgga atgccatgtg gggcgtcaaa
     1801 attgagcaga ccgaagacgg caggcattat gtcgcgggta ttggcctcag catggaggat
     1861 acggaggaag gcaaactgag ccagttcctg gttgccgcta accgtatcgc gtttattgac
     1921 ccggcaaacg ggaatgaaac gccgatgttt gtggcgcagg gcaaccagat attcatgaac
     1981 gaagtgttcc tgaagtatct gacggctccc accattacca gcggcggcaa tccgccggta
     2041 ttttccctga caccggacgg gcggttgacg gcgaaaaatg ccgatatcag cggtaacgtg
     2101 aatgcgaact cagggacgct caataatgtc acgattaacc agaactgtcg gattctggga
     2161 aaactgtctg ccaaccagat tgaaggtgat attgtcaaaa cggtgggaaa agcctttccg
     2221 agaaatggca gttatgccag cggtacaata acggtcactg tgtacgatga ccaggctttt
     2281 gaccgtcaga tagtaatccc acccgttctg tttcgcggtg gtaagcatga aaacttcaac
     2341 agcaacaacc aacagtcata ctggtattca acctgtaagc tgcaggtact gaagaacgga
     2401 caggaaatct ttcagcaacc cgcgacggat gtcagcaggg tattttcatc cgtcattgat
     2461 atgccagcgg gacacggcca tgtcaccctg actttcaatg tttcttcata tggtgcaaat
     2521 aactggacgc caacgaccag tatcagcgac cttcttgttg tcgtgatgaa gaaatcaaca
     2581 gccggtatca gtatcagttg aattttataa cccaaatacg ggcgccagaa atggcgcctt
     2641 ttttatttgt ggagtgaata tggcagtaca gatttcaggc gtgctgaaag acggtgcagg
     2701 aaaaccaata cagaactgta ctattcagct caaagcaaag cgtaacagca ccacggtact
     2761 ggtgaacacg gtggcctctg aaaatccgga tgaagccggg cgttacagca tggatgttga
     2821 gtatggccag tacagcgtca ccctgctggt tgaaggtttt ccgccttcac atgccgggac
     2881 cattaccgtc tatgaaggtt ccagaccagg tacgctgaat gattttctcg gtgccatgac
     2941 ggaagatgat gtcatgccgg aggcattgcg tcgttttgag gtaatggtgg aggatgtggc
     3001 acgcaacgct gaagccgcct ctcagagcgc agcggcagca aagaaatcag aaacagcagc
     3061 ggcatcgtcc aggaacgcgg cgaaaacatc agagacgaat gcaggtaaca gcgcgaaagc
     3121 ggcagcttct tcaaaaacag ccgcacaaaa cgcagcaaca gcggcagaac gttcagagac
     3181 aaatgcccgt gcgtcagaag aagcctccgc agacagtgaa gaggcttccc gccgtaatgc
     3241 agagtcagcc gctgaaaatg ccggagtcgc caccacaaaa gcgcgggagg ctgcagcaga
     3301 cgcaacaaag gccgggcaga aaaaggatga ggctctgtcg gcagcgacac gagctgaaaa
     3361 ggcggcagac cgcgcagaag ccgcagcgga agtgactgca gagccctgtg cgaatatagt
     3421 gccgccgctg cctgatgtgt ggataccgtt taacgattca ctggatatga ttgcgggttt
     3481 ttctccgggc tataaaaaaa tagctattgg tgacgatgtg gttcaggtcg ccagtgataa
     3541 acaggttaat ttcagtcgcg catcaacggc aacatatatc aacaaatctg gcgaactgaa
     3601 aacggcggaa attaatgagc cgcgatttga gtgtgatggc ctgcttattg agggacaaag
     3661 aacgaactac atgctcaatt cggaaagtcc agccagctgg gggaagtcat caaacatgga
     3721 tgtgcccgaa accgggacgg atagttttgg ttttacttat ggaaagtttg tctgcaacga
     3781 ttctctggtt gggcaaactt cggctattaa tatggcatca attgctgcaa caaagtcagt
     3841 tgatgtctca ggcgataaca agtacgtgac aacctcatgc cgttttaaaa cagaacgaca
     3901 ggtaaggtta cgtatacggt ttgataagta tgatggtagt gcaacaactt ttcttggcga
     3961 tgcgtacatt gatacgcaaa cgcttgaaat taatatgaca ggtggtgctg ccggcagaat
     4021 tacggcacga gtcaggaagg ataagaccac gggctggatt tttgcagagg caacgattca
     4081 ggcaattgat ggagagttaa aaataggctc tcagatacag tattctcctg agcagggtgg
     4141 ggcaacagta tctggtgact atatttatct tgccacccca caagtagaga atgggccatg
     4201 tgtatcatca tttattattt caggaggcag cgcaacgaca agagccagtg atttggttag
     4261 tatccccacc agaaataatc tttataagtt accatttact tttttacttg agattcataa
     4321 aaactgggat attgcaccaa acgccgcacc ccgcgtgtgg gatatagcag cagccaatac
     4381 cgggcaatca gcaattgcag caatcaacag aggtagtggt aagttatata tgagtctgtc
     4441 aaacccttca ggctcgtatg ttaatagcgc agcgacagat gtatttgcag agaaaaccac
     4501 atttggatgt attgcaaaag ctgatggtca ctttcatgtg gtgacaaatg gtaaagcggt
     4561 taatgaagtt tattgtgaat ataatggcgt gaccgctgat aaaaatatcc gatttggagg
     4621 gcagacgaat actggagaac gacatctgtt tggccatatt cgcaatttcc gcatatggca
     4681 taaagaatta aatgacaggc aattaaaaga ggtcgtatga aagatttaac tttgaagttt
     4741 catgacaaac tgcagtttaa ggccttcctg tcatctcttg gctgggcgga agatgaagac
     4801 ctccagaata aactgttagt tgatgaaatt ggtttcacct acacagaaac aggggtaaca
     4861 gaagagggag aacctgtctg tatccggaat gatggttatt ttgtcaacat tcgcattctt
     4921 gatgacttgt ttgatgtttc tgtattctct gattatgtcg tggagctgga aacaccgctt
     4981 cgggaatgga gctgaaagga ggaaataatg gatataagcc ccttacttca tgcactttgt
     5041 gctgtggctg cgcagatact ggttggtctt tttaccggaa actgggctta cggagcgata
     5101 gccggttgta cgttcttcat tgcgcgtgaa catacccagg cagaatatcg ctggattgaa
     5161 atgttcgggc atggcaagcg aatgaatatg ccgtggtggg gcggttttga tccgcgtgct
     5221 tgggatgtgg caagcctgat ggattttgct gtgccggtgg tggcgtgtct gctggtctgg
     5281 ctgttggtta atcgtgggcg aaaaaaggtg agctgtatat gcaacggagg aagaaacccc
     5341 gttgctggaa gccaggaaaa agtatcgggt gttgctgtcc tcggatgttg tgctaattgg
     5401 gtgataacag atcctgtgta tgggagtttc cctttttgcg tgcgttgatg ctggtaacgg
     5461 caaacgtcat gatgaacaaa accgcgaata agacgacaat cgtcggagct ggtgcgctgt
     5521 cgataaaaaa ggacaaccag acaccagtca ttgagaccaa tatcgatatg ccagtcgcca
     5581 gcaggagcgc aatatgaaag cgttgtgtca ggagtaccgc aatggcacca ggcgcaatga
     5641 gcagagaaat ggagagaatg atacctaccg ctttcagcgt cgccacaatg gtcagagaga
     5701 ccatgcacag cagcccataa tgcagccatc tcgtatgcaa accgcttacc tgcgcctgtt
     5761 gataatcaaa gctgaacagc aaaaaatccc gccattttac gctaataaca agtgtaacca
     5821 gcccggcaat aatcatcgtc tggattatat cgccgatggt tatcccgagc atgtcgccga
     5881 aaagaatatg gtcaagatgt acgtctggtt tgactgcaat atacaagatc agacctgcgg
     5941 caaacatgcc agaaaaaaca atccccatca ctgtatcttg cttgattcgg ctgttgtctt
     6001 tcaggtatcc cgtcgccacc gcgcagaata ctccggcgac gaatgcccct gtagccaaag
     6061 gtaaccccag aatccaggca agcacgatgc caggaaaaac cgcatgactc atcgcgtcac
     6121 ccatcagtgc ccaacctttc aggaccagaa agactgacag cagcgcacag ggtatggaaa
     6181 ctaccagtga aatcagcagt gcgtgactca taaaggtgaa ctgcagtggc tccagtaata
     6241 atgccatcat gagcgatcct ccagcgcctt atgcgcacgc cgacgatttg ccaaaagccc
     6301 atgggtgggc gcaaagacaa atgctaaaag aaacagcagt gtctgcgcca ccacgatgat
     6361 tccacctgtc gcgccgtcca gaaagtaact gacccacgcg ccgaggaaac tggttacact
     6421 gccaatagtt acagcaatca tgagcaggcg gggaaaacga tcggttaaca accatgctgt
     6481 tgcaccggga gtcaccacca gacagatcac cagaaaggca ccaaccgttt gcagcgccgc
     6541 aacggtcgaa accgacagca gggtgaagaa gaggagtttt aatctgcccg gatgcaatcc
     6601 aatagcacgg gcgtgattct cgtcgaaaaa ggtcaccata agatctttcc atttgaaaaa
     6661 aagcacgatg atcgagagaa tgccaatgat agtgagttgc aggatatccg ccggatcgat
     6721 tgccagaata ttgcccagga cgatggtctg aatgttcaca gatgtcgggt tcaatgacac
     6781 cataaacagc cccaggccaa aaaaggacga gaagatcaga ccgatgatgg catcttcttt
     6841 taagcgggtg cgttggttaa gaaacagcat gctgcccgca gccagtccgc cggaaaagaa
     6901 ggctcccaga gaaaacggaa gccccagcat atatgccccc gcaacacccg ggacaataga
     6961 gtgcgacaac gcatcgccaa tcagcgacca gccttttagc atcagatagc atgacaggaa
     7021 agcgcagaga ccgccgacca tcgccgagac ccacatcgca ttgagcatat attcatagct
     7081 gaagggttcc agtagcacgt tcatctttct tccctctgca ccgctgacgg tcgatgagcc
     7141 acaaaagggc gttcatcgtc agtaataatg ctttcttccg agccgttaag tgtgacatgg
     7201 cgcagtacgc cgctaaaagc cagctctagg ttctcggctg taaaagtggt gtctgtcggc
     7261 ccgcttgcca ggacggtgcc tttgaccatg accgtataat cgcaaaatgt cgtgacggat
     7321 ccgagattgt gtgttgagac aagcatcgtt ttgccttccg cgcgtaactc cctcaacagg
     7381 ctgatgattt tggcttcggt tttcacatca acgccagtaa aaggttcatc cagcaggatc
     7441 acatcaccct gctgtgcaat cgctctcgcc agaaagacgc gtttcttttg tccaccggaa
     7501 agctcgccga tttgtcgatg gcgaaaattc accatatcga cacgttccag cgcatcggtg
     7561 acaatttgac gatccctctt tttggcgata cgcaacatac ccatatgccc atagcggccc
     7621 atcatcacca catcttccac cagtacagga aatgaccagt caacctcttc tgactgaggg
     7681 acgtaggcaa ccaggttttt ctgtaacgcc tgtcgcgtgg gaatacccag aacagatatc
     7741 tttccgctgg tcagacgcac aaatcccata atcgctttaa acaacgtcga ttttccggaa
     7801 ccgtttaccc caaccagagc ggcaatagat ccacttggta ctgtgaagga tgcatcgcgc
     7861 agtgctgtgt gcccgttatg ccaggtgaca gcaacatcat taacgacaat gcctgcagat
     7921 tgcatcatta tttctccctt ttcccggctt taattccctg taccagcgta ctggtagtaa
     7981 ccttaagaag gtcgatatac gtcggtaccg ggccgttttc tgtgctcagg gaatcgacat
     8041 agagcacacc gccgtagtg
//
LOCUS       DAZFFE010000157         8025 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFFE010000157 DAZFFE010000000
VERSION     DAZFFE010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.158"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..552
                     /locus_tag="ACHJ69_004401"
     CDS             <1..552
                     /locus_tag="ACHJ69_004401"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017249.1"
                     /translation="SGGSSGSGSSGSGSGGSSGDDNGWLDGILGWLKTIWVSVNDLNN
                     AFSVSQADMNNALNSLDNGIKDISQNIASSLPDGDFPLGELSHYLPGQGSDSHDNNNW
                     LTVNIDKLVNTNNGTALPFTLQFSFNLPLIGAINFKIDTSHFAKAYDDYVRSVLEYAI
                     YMITMLRVFVISRRTLFEREVKL"
     gene            552..911
                     /locus_tag="ACHJ69_004402"
     CDS             552..911
                     /locus_tag="ACHJ69_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017250.1"
                     /translation="MWAILGAFFVDVVLKSLRFIAGVIGDKAVAWAKLLSYLGLCYTS
                     YYALVSAVNFIIRQISDVNKLDVIVVAFSYLPLNIQYCFNIILHVQFISYIYLYKDRV
                     YRLVYDLFKSNGLNVGK"
     gene            928..2139
                     /locus_tag="ACHJ69_004403"
     CDS             928..2139
                     /locus_tag="ACHJ69_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HFO5017251.1"
                     /translation="MPVITGNLGQGKGIVAAYFASLYYRRGLRVAANFPLNTEYMSSG
                     SDNPVTVIPAMPRIEDFELLGRGCPENEKTRFGALFLDECATWLNTRGFARKDRLPLI
                     DWLIHSRKLGWDVYLIAQHEDMIDSQIIKSMGAKIIRCRRLDELRVPVITPLMELFRP
                     GKTGVASGKRGIIPHYVAASTFLYDGTIHAARRPVDKIIIRAADYYNVYDTNFIFSDG
                     MELLNGRFVDMRAVYSVLPGRTLKTMNPLSQQPEKKATPQKKPWGKLIAFLFLVTAMI
                     AVARHYFSGDREASAAVPPVPESTVTPAHPLPASTVVSELRQKKPDVLPVSKEWRLAG
                     YVRGASPYFVLLGPAGQVRRYTAFQPWNGSATELNVDGEKVTFWSGSSGTASKGDVLT
                     DKMLSFDVNGK"
     gene            2587..3795
                     /locus_tag="ACHJ69_004404"
     CDS             2587..3795
                     /locus_tag="ACHJ69_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HFO5017252.1"
                     /translation="MMRLINLLTAFLFLTSACVHASSPAFFVDRIPLNAAIQLAQEDI
                     FRRQYALPPELASDTRPVTLDLSLTGDQKKQREEYVRWLRQLNISVETRNGVDHYRSF
                     KPVAAPEKLVSWVYTPFNRSPAYLAAVLSGTTTGHSSQVSSENAAASSPVTSGSFLSG
                     EGDSLVFRGTRSELARLKELVPLVDVPAQGVVVTGYIYEVQTGRSEGSGLALAAKLLS
                     GRFGVSVGSSSSMGNYISFSSGTLNAMYELFRTDNRFKVVSAPQLRMDSGKEATFSVG
                     EQVPVLGSVSYEDGKAVQSVTYRDSGVIFKVKPVITSSRISLNVNQQLSNFVKTDTGV
                     NDSPTLLKREVDTSLTLKDGDIVLLGGLAENKDSQANTGLSFLPKSWSQKSDEKSRTD
                     MVILLQVKKV"
     gene            4271..6550
                     /gene="ydeP"
                     /locus_tag="ACHJ69_004405"
     CDS             4271..6550
                     /gene="ydeP"
                     /locus_tag="ACHJ69_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO5017253.1"
                     /translation="MKKKIEFYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDDVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLDRLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTSSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            6798..6995
                     /gene="safA"
                     /locus_tag="ACHJ69_004406"
     CDS             6798..6995
                     /gene="safA"
                     /locus_tag="ACHJ69_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO5017254.1"
                     /translation="MHATIVKNKITQRDNYKGIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            7071..7832
                     /gene="ydeO"
                     /locus_tag="ACHJ69_004407"
     CDS             7071..7832
                     /gene="ydeO"
                     /locus_tag="ACHJ69_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO5017255.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTDNKMSALAI"
BASE COUNT         2092 a         1726 c         1934 g         2273 t
ORIGIN      
        1 tccggcggtt catccggtag cggtagttca ggttcaggat ccggtggctc atccggcgat
       61 gacaatggct ggcttgatgg tattttgggc tggttaaaaa caatatgggt gtcagtgaat
      121 gatttaaata atgctttttc tgtttctcag gctgatatga ataatgcatt aaattcactg
      181 gacaatggta ttaaagatat tagtcagaat atagcttctt ctttgcctga tggggatttc
      241 cctttagggg agctttcgca ttatttgcca ggacagggaa gtgatagtca tgataataac
      301 aattggctta cagttaatat tgacaaactg gtaaatacaa ataatggaac tgctcttcct
      361 tttactttac agtttagctt taatcttccg ttgattggcg cgattaattt caagattgat
      421 acaagccatt ttgcaaaagc ttatgacgat tatgttcgtt ctgttcttga atatgctatt
      481 tatatgataa ccatgttacg cgtttttgtt atttctcgcc gtacattatt tgaacgtgag
      541 gtaaaactct gatgtgggcc attttaggtg ctttttttgt tgatgttgtt ttaaaatcac
      601 tacgtttcat tgcaggagtt atcggtgata aagctgttgc atgggctaag ctcctttctt
      661 atcttggtct ttgctacact tcatattatg ctcttgtatc tgccgtcaat tttattattc
      721 gtcaaatctc cgatgttaat aaactcgatg ttattgtagt tgcattcagt tacctgcctt
      781 taaatattca gtactgcttt aatatcatcc tccatgttca atttatttca tacatatatc
      841 tttataagga tagagtgtac cgattggttt atgatttatt taaatcaaat gggcttaatg
      901 ttggcaagta attttgcggg gtaagttatg ccagttatca ccggaaattt ggggcagggt
      961 aaaggtattg tcgcagctta ttttgcctca ctttattatc gccgtggatt acgggttgcg
     1021 gcaaattttc cgcttaacac tgaatatatg tcctcaggtt cggataaccc cgttacggtt
     1081 attccggcta tgccccgcat tgaagacttt gagcttcttg gtcgtggatg ccctgaaaat
     1141 gaaaaaacac gcttcggggc gttatttctt gatgagtgtg cgacatggct gaatacccgt
     1201 ggttttgccc gtaaggacag attaccgtta attgactggc tgatacattc ccgtaagctg
     1261 ggctgggatg tttatctgat tgcacagcat gaagatatga ttgattccca gataattaag
     1321 tcgatggggg ctaaaattat tcgctgccgc cgtcttgatg aattacgtgt tcctgtgatt
     1381 acgccactga tggaattatt cagaccagga aaaacagggg ttgcatcagg caaaaggggg
     1441 attattcctc attacgtggc tgccagtacg tttctttatg acggcacgat tcacgcggca
     1501 cgtcgtcccg tagataaaat cattatcagg gctgcggatt attacaacgt ttacgacact
     1561 aatttcattt tctctgacgg aatggagctg cttaatggcc gttttgtgga tatgcgtgct
     1621 gtttattccg ttttgcccgg gcgcacactt aaaacaatga atccgttatc tcaacaaccc
     1681 gaaaaaaagg ccaccccaca gaaaaagccg tggggcaagc tgattgcctt tcttttcctc
     1741 gtcaccgcga tgattgccgt tgcccgacat tatttctccg gcgatcggga ggctagtgcc
     1801 gctgtccctc ctgtgcctga aagcaccgtg acacctgcac atcctttgcc tgcgtctacg
     1861 gttgtatcag agctgagaca gaaaaaacca gatgtgcttc cggtatcaaa ggagtggcgg
     1921 cttgccggat atgtcagggg agcaagtcct tattttgtgc tgctgggtcc ggcgggtcag
     1981 gtgcgacgtt atactgcttt tcagccatgg aacggcagcg ccacggaact gaatgttgac
     2041 ggtgaaaaag tgacgttctg gtcaggctca tcgggaacgg catcaaaggg ggatgtgctg
     2101 actgacaaaa tgctttcgtt tgatgtgaat ggtaaataag acagaggaca ctccgctcgc
     2161 cgcagaccca gcgaccgaac gtagtgagcg agtggtcgag gaagcggaaa ggcaccatta
     2221 ccggccccgg gcacttacgc tctgctgcct gcttcgggca cttatgctct ttgctgacat
     2281 gggcaaccag cttactggct gtgcagaacc cgacgcgtac aggcgatgat gttgcctgtt
     2341 gatgaaactg atggctgaca gcgtgctccc ctgtagtgtg caccgcagct taatccgcgc
     2401 tttgttttca tggactgcac ctgcctgccg gactggcagg tctgacggat gaacagacag
     2461 gcagaatgct tcatcttcgc gtgtggtggt accgctcgcc gcagatccgg cgaccgaacg
     2521 cagtgagcga gtgatcgagg aagcggaata tcttcatgac tgaaaaacag cacttaacgg
     2581 tataacatga tgagattgat taacttactg acagcatttc tttttttgac ttcggcgtgt
     2641 gtgcatgcct cgtctccggc cttttttgtt gaccgcattc cgctcaatgc agcgatacag
     2701 ctggctcagg aagatatttt caggcgtcag tatgcgttgc cgcctgaact tgccagcgat
     2761 acccgtccgg tgacgctgga cttaagtctg acgggcgatc agaaaaaaca gcgtgaagag
     2821 tatgtgcgct ggctcagaca actgaatatt tctgttgaga ctcgtaatgg cgtggatcat
     2881 tatcgctcgt ttaaacccgt ggctgcgccg gaaaaactgg tttcatgggt atatacacca
     2941 tttaaccgtt cgccagctta tcttgccgcc gtgctttcgg gtacaacgac agggcattca
     3001 tcacaggtat cttcagagaa tgcggctgca tcttctccgg taacttccgg ctcattctta
     3061 tcaggtgagg gtgattcact ggttttcagg gggacacgtt cagaactggc acgccttaag
     3121 gagctggttc cgctggttga tgttccggct cagggggttg tggtaacggg gtatatttat
     3181 gaggtgcaga cagggcgctc tgaaggttcg gggctggcac tggctgcaaa actgttatca
     3241 gggcgttttg gtgtcagcgt gggttcctca tcctcgatgg ggaattacat ttcattcagc
     3301 tccggtacgc tgaatgcgat gtatgaactg ttcagaacag ataaccgctt taaggtggta
     3361 tccgcgccgc agctgcgcat ggattcaggc aaggaagcga cgttttctgt tggtgaacag
     3421 gttccggttc ttggcagcgt gtcttatgag gatggtaaag ccgttcagtc cgtgacgtat
     3481 cgtgattcgg gggtgatatt caaagttaag ccggttatca cgtcgtcgcg tatcagcctg
     3541 aacgtcaatc agcagttaag taactttgtc aaaacggata cgggcgtgaa tgattcgcca
     3601 acgttgctca agcgtgaggt ggatacgtcg ctgactctga aagatggcga tattgtgttg
     3661 ctgggtggtc tggcagaaaa caaggattcg caggccaaca caggattatc atttctgcca
     3721 aagagctgga gtcagaagtc tgacgagaag agccgcacgg atatggttat tctgttacag
     3781 gtcaaaaaag tttgagtgcc gaccgggatt atcaggacgc ggcatctcag tttacccacc
     3841 acaacgtcag tgcgcataat gcatattatg ttaaatcatg tctgagtgat acgaatacgt
     3901 tgtgatttct gacttccctc ccgtttcggt agaaaaattc aacctctgtt cagtgatggc
     3961 acaccataga ccatgagttg gatacttgcg ttgttttgac attagtctat gcgctccatt
     4021 cacgaaaatt gcgcggataa ttattttgtg aaggctatta tcctacactt gtaagaaaat
     4081 ccgcgcacaa ttgaagccag agtcttattt tttatattga gaattatgtt aatttaaacc
     4141 ctatgagaga taatagtaag agagtagtcc cctactctta tatattcatg ttgacgataa
     4201 accctttgta tatactttgc atcatcgcta ttacaaatcc taataattca tttccacaca
     4261 ggataagcag atgaagaaaa aaattgaatt ctaccaggga gctgccggtg gttggggtgc
     4321 tgttaaatcc gtagcgaatg cagtacgtaa gcagatggat atacgccagg atgttattgc
     4381 catgtttgac atgaataagc cagagggctt tgactgtccg ggatgtgcat ggccagatcc
     4441 taagcacagt gcgtcattcg acatttgtga aaacggcgca aaagcaatcg cctgggaagt
     4501 cacggataag caggtaaatg cctctttctt tgctgagaat acggttcaat cattacttac
     4561 ctggggagat cacgagcttg aggctgcggg acgtctcact cagcctttga aatatgatga
     4621 cgtcagcgac tgttacaagc cattaagctg gcaacaagct tttgacgaaa ttggcgctcg
     4681 ccttcaaagc tatagtgatc ccaatcaggt tgaattctat acttcggggc gcacttccaa
     4741 tgaagctgcc tttctttatc agctttttgc ccgtgaatac gggagcaata actttcccga
     4801 ctgctccaac atgtgccatg aaccgaccag tgtgggattg gcagcgagta tcggtgtggg
     4861 taaagggacc gtgttgctgg aagactttga gaaatgcgat ttagtcattt gcattgggca
     4921 taaccctggt acaaaccacc ctcgcatgct gacttcgttg cgcgctttag tgaaacgggg
     4981 agcgaaaatg atcgctatca accctctaca ggaacgtggc ctggagcgat ttaccgcacc
     5041 gcaaaacccg tttgaaatgc tgacgaactc tgagactcag ttggccagtg cctactataa
     5101 cgtgcgcatt ggtggcgata tggcattgct caaggggatg atgcgcctgt taattgagcg
     5161 cgatgatgct gcaagcgccg caggtcggcc ctcattgctg gatgacgaat ttattcaaac
     5221 gcataccgtc ggctttgacg agctacgccg tgacgtgctc aattccgagt ggaaagatat
     5281 cgaacgtatt tctggactaa gtcagacaca aatcgccgaa cttgctgatg catatgccgc
     5341 tgccgaacga actattatct gttacggaat ggggatcact cagcacgaac atggtaccca
     5401 gaacgtacag caactggtca atctgctgtt gatgaaaggt aacattggca agcctggtgc
     5461 gggtatctgc ccactacgag gtcactctaa tgtacagggc gaccgaaccg tcggtatcac
     5521 cgagaaaccg tctgcagagt ttctggatcg cctgggtgag cgctatggct tcaccccacc
     5581 tcatgcacct ggacatgctg caatcgccag catgcaagct atatgtacgg ggcaagctcg
     5641 agcattgatc tgcatggggg gcaactttgc actggcaatg ccagatcggg aagcgagcgc
     5701 tgtaccgtta acgcaattag atctggcggt acacgtagcc actaagctta accgctctca
     5761 tctgctgacc gcacggcata gctatattct gccggttctg ggacgtagcg agattgacat
     5821 gcaaaaaagc ggtgcgcagg cggtaaccgt tgaagattca atgtcgatga ttcatgcctc
     5881 gcgtggtgtg ttaaaacccg ccggtgtaat gctgaaatca gagtgtgcag tggtcgcggg
     5941 aatcgcgcaa gcagcactac cccagagcgt ggtagcctgg gagtatctgg tggaagatta
     6001 tgatcgcatt cgcaatgaca ttgaagctgt gctgccagag tttgccgact ataaccagcg
     6061 catccgtcat cctggtggtt ttcacctgat aaacgcagca gctgaaaggc gctggatgac
     6121 gtcgtcaggt aaggctaatt tcattaccag taaagggctg ttagaagatc cctcttcagc
     6181 gtttaacagt aagctggtca tggcgacagt acgcagtcac gatcagtaca acacgacgat
     6241 ttatggtatg gatgatcgct atcgaggggt attcggtcaa cgagatgtgg tctttatgag
     6301 tgctaaacaa gctaaaattt gccgtgtaaa aaacggcgaa agagttaatc ttattgcact
     6361 cacgccagac ggtaaacgta gctcacgccg catggataga ttaaaagtgg tcatttaccc
     6421 tatggctgac cgctcactgg tgacctattt tccagaatcg aatcacatgc taacacttga
     6481 taaccacgat ccattaagtg gcattcctgg ctataaaagt attccggttg aattagaacc
     6541 atcaaattaa ttcgcctcct catttcttct gctgtcatcc gcacagcaga agaattcctc
     6601 attgactatt atcgcgcaat ttgctcacat ggattaaatt aaaccacaca ctataagata
     6661 taaacttctg cctacagctg taagaaactc cgctcagtac tgaagtaccc gtcctatttc
     6721 ctctattctc cagcctgtta tattaagcat actgattaac gatgcttaac gtcattcgtt
     6781 taataaacac atttgaaatg catgcgacca tagtgaaaaa taaaatcacg caaagagaca
     6841 actataaagg gatcatgtct gtaattgtgg ttgtcttatt actgaccctt acattgatag
     6901 ccattttttc ggcaattgat cagctgggta tttcagaaat gggtcgcatt gcaagggatc
     6961 ttacacattt cattatcaat agtttgcaag actgaaaaac aaacagcaaa ttataaatat
     7021 gaaatgttaa aaaagtatcg ataaaaactt tattgtttta aggagataaa atgtcgctcg
     7081 tttgttctgt tatatttatt catcatgcct tcaacgctaa cattttagat aaagattacg
     7141 ccttctctga cggcgagatc ctaatggtag ataacgctgt tcgtacgcat tttgaacctt
     7201 atgagcggca tttcaaagaa atcggattta atgaaaatac cattaaaaaa tatctacaat
     7261 gcactaacat ccagacagtg acgatgcctg ttcctgcgaa gtttttacgt gcttcaaatg
     7321 taccgactgg attgcttaat gaaatgattg cttatctcaa ctcggaagaa cgcaatcatc
     7381 ataatttttc agaacttttg cttttttctt gcctgtctat ttttgccgca tgcaaaggtt
     7441 tcattacact attaactaac ggtgtgctat ccgtttccgg gaaagtgaga aatattgtca
     7501 acatgaagct ggcgcaccca tggaagctga aagatatttg tgactgcctg tacatcagtg
     7561 aaagcctgtt gaagaaaaaa ctaaagcaag agcaaacgac attctcacag attcttttag
     7621 atgccagaat gcagcacgca aaaaatttga tacgcgtaga aggttcagtc aataaaattg
     7681 ccgaacaatg tggttatgcg agtacatctt attttattta tgcgttccgc aaacatttcg
     7741 gcaacagtcc aaagagagtt tctaaggagt accgttgtca aaggcacacg ggtatgaaca
     7801 cggataacaa gatgagtgct ttagctattt gattatttgc taacgagtag tcaaccacac
     7861 acgctgcgta agaattaaat ggggcagcca ttccctgccc cgcgttgttt ttaggtgata
     7921 tatttcttga aataaataag tgacagcctt cacatattta tgcatttgca taacccgttg
     7981 catgattatt tatgatctca attcggtatt ttgtcggtaa aattc
//
LOCUS       DAZFFE010000158         7842 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZFFE010000158 DAZFFE010000000
VERSION     DAZFFE010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.159"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..491
                     /locus_tag="ACHJ69_004408"
     CDS             3..491
                     /locus_tag="ACHJ69_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO5017256.1"
                     /translation="MLKMSLYVIILLFSLQFSAAITGKESEVVSPLLMDVNPSLTMEN
                     ISELSTSSEPSQQGVFPVICTRLHPGSVMKRQLLTGWGPVFIIGDDPFSLRWMSEHLE
                     ILKSLNALGLVVNVESVERMEVLQQRADGLLLLPVICDNFVQALQLNAYPVLITEMEI
                     SQ"
     gene            517..711
                     /locus_tag="ACHJ69_004409"
                     /pseudo
     CDS             517..711
                     /locus_tag="ACHJ69_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(762..1532)
                     /gene="yafV"
                     /locus_tag="ACHJ69_004410"
     CDS             complement(762..1532)
                     /gene="yafV"
                     /locus_tag="ACHJ69_004410"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO5017257.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESDSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNERVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYKEKFPAWRDADEFRLW"
     gene            1713..2159
                     /gene="ivy"
                     /locus_tag="ACHJ69_004411"
     CDS             1713..2159
                     /gene="ivy"
                     /locus_tag="ACHJ69_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO5017258.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(2202..4646)
                     /gene="fadE"
                     /locus_tag="ACHJ69_004412"
     CDS             complement(2202..4646)
                     /gene="fadE"
                     /locus_tag="ACHJ69_004412"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO5017259.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAVLGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLLRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAYIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4886..5464
                     /gene="lpcA"
                     /locus_tag="ACHJ69_004413"
     CDS             4886..5464
                     /gene="lpcA"
                     /locus_tag="ACHJ69_004413"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO5017260.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5669..6436
                     /gene="yafJ"
                     /locus_tag="ACHJ69_004414"
     CDS             5669..6436
                     /gene="yafJ"
                     /locus_tag="ACHJ69_004414"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO5017261.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGESVV"
     gene            complement(6407..7147)
                     /gene="dpaA"
                     /locus_tag="ACHJ69_004415"
     CDS             complement(6407..7147)
                     /gene="dpaA"
                     /locus_tag="ACHJ69_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO5017262.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            7459..>7842
                     /locus_tag="ACHJ69_004416"
     CDS             7459..>7842
                     /locus_tag="ACHJ69_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HFO5017263.1"
                     /translation="MSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAKSRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LVENNHFLSSDHLHNITEVAIHRLEQ"
BASE COUNT         1940 a         2051 c         1937 g         1914 t
ORIGIN      
        1 ttatgttaaa aatgagttta tacgtaataa tattgttatt ttcccttcag tttagtgcag
       61 ccattactgg caaggaaagt gaggttgttt caccattatt aatggatgtt aatccttcac
      121 taacaatgga aaatatttct gaattatcca catcatcgga accatcgcaa cagggggtat
      181 ttcctgtaat atgtactcga cttcatcctg gctccgtaat gaaacgtcag ttattaaccg
      241 gctgggggcc tgtttttata attggtgatg atcctttttc tctgcgctgg atgagtgaac
      301 atctggagat ccttaaatcg ctaaatgccc ttggtttggt tgttaatgtt gaatcagtag
      361 aacgaatgga ggtattacag cagcgggctg atgggttatt gttattgcca gttatctgcg
      421 ataattttgt gcaagctctg caattaaatg cttatccggt attaatcaca gagatggaaa
      481 tatcacaatg attaataaca taattactgc taataatatg gtgattttga aaatggtaat
      541 cctgttcacg acaccataat cagagttatg tcctgtatcc gttcagtaaa atttcacgaa
      601 tgctttatta accggatgcg tgaatgtcat tcttcagacg acatagacgt cattgcgatt
      661 gatggaaaag cacttccaca ctcctgtgat aaaagtcgtc gcaggagagc gggctttcgt
      721 agtcttcccc gactctcccc ggccctaaac acaaccccca ctcaccacaa cctaaactca
      781 tccgcatccc gccatgccgg aaacttttct ttatattccc gcaatgccat catcgacagc
      841 tccgcatcaa tgcgcgttgc ctgatgcgcg tcggcagtag cgataatctc gccttgtgga
      901 ttaatcaccc ggctgtcacc gcgataatgg cagccgttgc catcgctgcc gacgcgatta
      961 catcccgcta catacgcctg attctcaatc gcgcgggctg tcagcaatgc ctgccagtgc
     1021 agagaacgcg gtgcaggcca gttggcaacg tagatggcga ggtcataatc gttgagattg
     1081 cgcgaccaca ccggaaaacg taagtcgtag cacaccagcg gtaaaatacg ccagccgcgc
     1141 cattccacaa tcactcgctc attgcccgct ttataatgca gatgctcatc tgccatgcgg
     1201 aacagatgac gcttatcata aaaatgtacc gtgccgcccg gctcaaccag caaaaagcgg
     1261 ttaaccgaat cagactccgt ttgtaatgca acactgcctg caatcagcgc attgcactgc
     1321 tgcgctttag cggtcatcca gttcactacg tcatcttgtg ctagcgacga agctgccgct
     1381 tccatggcaa agccgctggt aaacatctcc ggtagaacga tcacatcgcg cccggtaata
     1441 ccttccagtt gacgatcaaa atggcgcagg ttggcaggac catccatcca caccagtggt
     1501 tgctgcaaaa gcgtaatctt caaaccaggc acggtgtaca actcctttat gcgaagggtt
     1561 ttataactct aacaccttat caggcagttg ccttagcgca gaataaattg ataacaaatg
     1621 ctgatattgg aaatatctga tttgcaaatt atcgtgttat cgccaggctt tagggagtta
     1681 atcacatggg caggataagc ccgggaggaa tgatgtttaa ggcaataacg acagtcgccg
     1741 ctctggtcat cgccaccagt gcaatggcgc aggatgattt aaccattagc agtcttgcaa
     1801 agggcgaaac caccaaagcg gcgtttaatc agatggtgca agggcataag ctgcctgcct
     1861 gggtgatgaa aggcggtact tatactcccg cacaaaccgt gactttggga gatgagacgt
     1921 atcaggtgat gagcgcgtgc aaaccgcatg actgtggctc gcaacgtatc gctgtgatgt
     1981 ggtccgagaa atctaatcag atgacggggc tgttctcgac tattgatgag aaaacgtcgc
     2041 aagagaaact cacctggttg aatgtgaacg atgcgctttc gattgatggc aaaacggtgc
     2101 tgttcgcggc gttgaccggc agcctggaaa accatccgga tggctttaat tttaaataat
     2161 tagccgataa aaaaacggag ccgaaaggct ccgttattca attacgcggc ttcaactttc
     2221 cgcactttct ccggcaactt taccggcttc gtcgccagct cttctggatc aaagtcatca
     2281 acgttaatac tgcgtaaacg gctttcttca gcttttacca gaatagcggc ttcatcttta
     2341 tcaatcagcc ccttcgccag cgcgttgtgc gccagttcat ccagacgggt aaacggcagg
     2401 tttttaccca actctttaca gatccgctga tgaatcgggt cggcggcaat cacatccacc
     2461 agcgcctctt ccagtaaacc aaccgggtta tgctcgcttg gcgtcaggta ctgaccgcga
     2521 ccaatacgtg aacgagtggc gttcggtact tgtaaaatct tcgccacttt atgatccagc
     2581 ttgtcagacg gtgccaggta atgacgtccg gtcgggaaga tcaccacatt cagcagcccg
     2641 gcaaccacac ggttcgggaa gttttgcagt aaatcgtcca tcgcctgttc tgcctgatac
     2701 agcgcatcct gtacgcccca gtgcaccagc ggcagatcgg cttcattacg gccttcgtcg
     2761 tcataacgct tcagaacggc agaggcgagg taaagctggc ttaaaatatc ccccagacgg
     2821 gccgagatac gctcacgacg tttcaggctg ccgcccagca ccgccatcga gacatcagaa
     2881 agcagggcga ggttggcgct caggcggttc aggtgctgat agtagcgttt agtggcatcg
     2941 ccggttggtg tgctgctggt taaaccgcgc gtcaggccca gccagaagct gcgaactttg
     3001 ttgctaccga cgtgaccgat atgtttgaac agcagtttat cgaacgcatt tacgtcattg
     3061 ttcttcgccg cttccatctc ttccagcacg tacggatggc aacgaattgc tccttgtccg
     3121 aagatcatca tgctgcgggt cagaatgtta gccccttcaa cggtgatggc aatcggtgcg
     3181 ccctggtaag cacgggccag gaagttgctt tgcccgagca taatgccttt gcccccggtg
     3241 atatccatcg catcaataat cgactgctgc ccgcggtggg tacagtgata cttaacgata
     3301 gccgacagca cggcaggttt ttcaccgagc ataatgccgt aggtaatcag cgatgccgca
     3361 gcatccatta cataagcgtt cccggcaata cgcgccagcg gctcttcaat cccttccatc
     3421 ttaccgatag agattttgaa ctgccgacga atgtacgcat acgcgcccgt tgccagcgct
     3481 accgatttca cgccgccggt tgagttggaa ggcagggtga tgccgcggcc taccgagagg
     3541 cactccacca gcatccgcca gccttgcccg gccattttcg gcccgccgat gatgtaatcg
     3601 atcggcacga acacatcttt accgcgcgtc ggtccgttct ggaacggtac gttcagcggg
     3661 aagtggcgac gaccaatttc cacgcccggc gtggtggtcg ggatcagcgc acaggtaata
     3721 cctaaatctt ctgcgccacc cagcagtttc tccgggtcgg agagtttaaa cgccagacca
     3781 agcacggtcg caatcggtgc cagcgtaatg tagcgtttgt tccaggtcag acgcatcccc
     3841 agcacctgct ggccctgcca ttcgcccatg cagacaatcc cggtatccgg aatcgcgccc
     3901 gcatcggaac ccgcttccgg gctggtcagt gcaaagcaag ggatctcctg accacgcgcc
     3961 agacgcagca gatagtgatt tttctgctcg tcagtgccgt aatgttgcag cagctcgccc
     4021 gggcctaatg agttcggcac gccgacggta atcgccagga tcccgctcac gccggagagt
     4081 ttttgcagca cgcgagactg ggcataagcc gagaactcca gcccgccgta ctcttttttg
     4141 atgatcatcg cgaagaaacg atgctcttta aggtacgccc acaactccgg cggcaggtcc
     4201 gccagctcat gagtaatctg gaaatcattc gccatccggc aggcttcttc taccggaccg
     4261 tcgagaaacg cttgttcttc agcggtcagg cgcggctgcg gatagttatg cagctttttc
     4321 cagtccggct tgccctggaa caagtcgccc tcccaccagg tggtgcccgc atcaatcgct
     4381 tctttctcag tgcgcgacat cggcggcatc accttacgga aaccgcgaaa taccggtgcg
     4441 gaaatcatcg acttacgcat aggcgcaaag ttaaatggca cgaggataat ggccagaggc
     4501 actagtaccc acgccgacca cagaccagca acgccgagga cagctgtcca ggcgagcaaa
     4561 attagactgc tgataaataa gctcacgcgg tgatagaaca atgcgccgag caggacaacc
     4621 gtagcgagaa tactcaaaat catcataacg aaaagcccct tacttgtagg aggtctgacc
     4681 acttgtgatg atatggttgt agtggatgtg aaaatattta gcaatgtgtt tacaatataa
     4741 ttacaacaaa gctcacattg ttgctgtttt tatccgcact tcaggtcaaa aagtcctggt
     4801 catagcacct gcccgtactt ctcgcttttg gcggtatccg gtacactgca ttttgtctat
     4861 tacatttatg ctgaaggata tcctcatgta ccaggatctt attcgtaatg aactgaacga
     4921 agcggcggaa acgctggcta actttttaaa agatgacgcc aatattcacg ccattcagcg
     4981 cgcggcggtc ctgttagcag acagctttaa agccggtggc aaagtgcttt cctgtggcaa
     5041 cggcggctcc cattgcgacg ccatgcactt tgccgaagag ttgaccggtc gctaccgtga
     5101 aaaccgtccg ggctacccgg cgattgccat ttctgacgtt agtcatattt cctgcgtcgg
     5161 taacgatttt ggtttcaatg atattttctc ccgttacgtt gaagcggtag gtcgcgaagg
     5221 cgatgtactg ctggggatct ctacctccgg taactctgca aacgtgatca aagcgatcgc
     5281 agcggcgcgt gagaagggga tgaaagtgat caccctgacc ggtaaagatg gcggcaaaat
     5341 ggctggcacg gcggatattg aaattcgcgt tccgcacttt ggttatgccg accgcattca
     5401 ggagatccac attaaagtga tccatatcct gatccagtta attgaaaaag agatggttaa
     5461 gtaagtctgg cgtaggccgg ataaggcgtt tacgccgcat ccggcatttg tgctctgatg
     5521 cctgatgcga cgctgacgcg tcttatcatg cctacaatct gcacccgaac cgtaggccga
     5581 ataatgcgtt cacgccacat ccgacctgaa aattcttaaa tcaatcttcg ccgggggcca
     5641 tgcgctcccg ctgttgtgga ggttacccat gtgcgaattg ctcgggatga gtgccaacgt
     5701 ccctaccgat atctgcttta gtttcaccgg acttgtacag cgtggtggtg gaaccgggcc
     5761 acataaagat ggctggggca ttacctttta cgaaggtaaa ggctgtcgca catttaaaga
     5821 tccgcaaccc agctttaatt cccccatcgc caaacttgtc caggactacc cgataaaatc
     5881 ctgttcggtg gtggctcata ttcgccaggc taatcggggc gaggtggcgc tggaaaatac
     5941 tcacccgttt acccgcgagt tatgggggcg taactggact tatgcccata atggacaact
     6001 gacgggctac aaatcactgg aaaccggcaa cttccgcccg gtcggtgaaa ccgatagcga
     6061 aaaagccttt tgctggctcc tgcataaatt aacgcagcgt tacccgcgca caccgggcaa
     6121 catggcggca gtgtttaaat atatcgcctc actggcggat gaactgcggc agaagggcgt
     6181 tttcaacatg ctactttcgg atgggcgcta tgtaatggcg tattgctcga ctaatttaca
     6241 ctggatcacc cgccgcgcgc cgtttggcgt ggcaacgttg ctggatcagg atgtggaaat
     6301 cgacttcagc tcgcagacca caccgaatga tgtggtcacg gtgattgcga cacagccgct
     6361 gacgggcaat gaaacctggc aaaagattat gccaggtgaa tggcgcttat tttgcctcgg
     6421 ggagagtgta gtttgatgcc agttgtggct gcacaacttc gtggcttaac ggcttgctga
     6481 ccacgtaacg gccattgacc acagaaacgg tcggtggctt acgggtttgc tcaaagtagt
     6541 cgtagcctgg cttcagttgc tcccagaagt ccttaaagtt ggaatattta tggcgcttca
     6601 tattggcgtc ggtcatacgg aacgggtaaa tactcacttg cacgctcggc tgaccaaaca
     6661 ccagcgcacc agtaacgaac tggaagatct catcaatacc ctgattggtc atcgcgtagc
     6721 agccgatgga aacacaatcg ccgtgaatca tcaggtattt cccttcataa ccatgcgcac
     6781 ggtcataggc gtttgggaat ccaatattaa tcgctttgta gtaacggctg tctggtttta
     6841 actgattacg ctggacgcta taaaaccctt ccgggctttt gaaatcgccc tgacgctgtt
     6901 ttggccctaa gccgccggaa tatttacaga ttttatagct gtcgagcagt tgatattgct
     6961 cgcccatttt gacgtagaga tcgagcgtac gttcttcctt gaagatctgg atgtagacag
     7021 gggatcccat caactgctgc ttatactctt tgctcactgg cgtggtcgag ctactgctgc
     7081 ccagcaaacc ggcaaacgaa acgcacggga tcaacaacat cgcaagaatt aatgcgattt
     7141 tacgcatact gcttattcct tgataaaacg gttacacacg ccaggacggc aaaatgaatc
     7201 ccaaatcgga atagtctgga tttggaaggc tcacattatc accaaaagag ttttacgcaa
     7261 gcctgtcggc gcagggttta caaatttagc acaggggcag ttttcaagag tccgaaaagc
     7321 gcatggattg tagatttatt tcttatttcc taagattatg aaaccgcaat agtaatatgc
     7381 aaataagcaa ttaataatcc cgtttactcc tccggtcttt taaaattctc accttcattc
     7441 agctggttta tgtccttcat gtcttcgttc ttgcacggca gatttctgca tccaggcgtg
     7501 ttttcgttat gcgttttgct ccctttactc gccagcgcca ccacatcaca tatctctttt
     7561 agctacgccg cccgccagcg gatgcaaaac cgtgcgcgtt tattaaaaca gtaccaaacc
     7621 catctgaaaa agcaggccag ctatattgtg gaaggcaatg ccaaaagcag aagggcgctg
     7681 cgccagcata atcgggagca gataaaacag catccagaat ggtttcctgc gccgctcaag
     7741 gcgagtgaca gacgctggca ggcgttggtg gagaataatc atttcttaag cagcgaccat
     7801 ctacataaca tcaccgaagt ggcgattcac cgcctggagc ag
//
LOCUS       DAZFFE010000159         7737 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZFFE010000159 DAZFFE010000000
VERSION     DAZFFE010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.160"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..196
                     /locus_tag="ACHJ69_004417"
     CDS             <1..196
                     /locus_tag="ACHJ69_004417"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017264.1"
                     /translation="SAGCGVNALSGLQKKCRKSLKTAPDTKTVFKGSLADAENTTSPQ
                     QVNPGTHIRYEMCVVVSTAK"
     gene            225..3026
                     /gene="sucA"
                     /locus_tag="ACHJ69_004418"
     CDS             225..3026
                     /gene="sucA"
                     /locus_tag="ACHJ69_004418"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO5017265.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            3041..4258
                     /gene="odhB"
                     /locus_tag="ACHJ69_004419"
     CDS             3041..4258
                     /gene="odhB"
                     /locus_tag="ACHJ69_004419"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO5017266.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAPAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            4352..5518
                     /gene="sucC"
                     /locus_tag="ACHJ69_004420"
     CDS             4352..5518
                     /gene="sucC"
                     /locus_tag="ACHJ69_004420"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO5017267.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            5518..6387
                     /gene="sucD"
                     /locus_tag="ACHJ69_004421"
     CDS             5518..6387
                     /gene="sucD"
                     /locus_tag="ACHJ69_004421"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO5017268.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            6627..>7737
                     /locus_tag="ACHJ69_004422"
     CDS             6627..>7737
                     /locus_tag="ACHJ69_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO5017269.1"
                     /translation="MGVNSDVYAADVNIDILSATVKDKRIEGVSVTLQRNGAQSVSGT
                     TNASGSVNLGSTFADDQDALLIVKKEGYSNLVVKCSCAGMTYAISPAMTSLDGMRVVL
                     SWGEKPFDLDSHLIFSGGHIYFDSKEGTDANLDVDDTDSYGPETVTISKKHFGESYIY
                     AVQDYSNKGLPNSNYLSASKAKVFVYVGSSLVRSYSVPAGKRGNIWTVFKLNPNGEFE
                     DINSVTSANFNDTTLDVRDLATVIMPATDSSAPASPAMQNSGDTQLARKYNREGEAVY
                     KTGQLEQAIQLFQQATELDGNYGQAFSNLGLAYQKNGNIAEAIWANRKAISLASGANA
                     ATTRANSYYNIAKIYETAGQDADALQHYQLALHDAI"
BASE COUNT         1936 a         1960 c         2118 g         1723 t
ORIGIN      
        1 cagcgccgga tgcggcgtga atgccttatc cggcctacaa aagaaatgca ggaaatcttt
       61 aaaaactgcc cctgacacta agacagtttt taaaggttcc ttagcggacg cagaaaacac
      121 gacaagtccg caacaagtga accccggcac gcacatcaga tatgaaatgt gcgtggtagt
      181 atccacggcg aagtaagcat aaaaaagatg cttaagggat cacgatgcag aacagcgctt
      241 tgaaagcctg gttggactct tcttacctct ctggcgcaaa ccagagctgg atagaacagc
      301 tctatgaaga cttcttaacc gatcctgact cggttgacgc taactggcgt tcgacgttcc
      361 agcagttacc tggtacggga gtcaaaccgg atcaattcca ctctcaaacg cgtgaatatt
      421 tccgccgcct ggcgaaagac gcttcacgtt actcttcaac gatctccgac cctgacacca
      481 atgtgaagca ggttaaagtc ctgcagctca ttaacgcata ccgcttccgt ggtcaccagc
      541 atgcgaatct cgatccgctg ggactgtggc agcaagataa agtggccgat ctggatccgt
      601 ctttccacga tctgaccgaa gcagacttcc aggagacctt caacgtcggt tcatttgcca
      661 gcggcaaaga aaccatgaaa ctcggcgaac tgctggaagc actcaagcaa acctactgcg
      721 gcccgattgg tgccgagtat atgcacatta ccagcactga agaaaaacgc tggatccaac
      781 agcgtattga gtctggtcgc gcgactttca atagcgaaga gaaaaaacgc ttcttaagcg
      841 aactgaccgc cgctgaaggc cttgaacgtt accttggcgc aaaattccct ggcgcaaaac
      901 gcttctcgct ggaaggcggt gacgcgttaa tcccgatgct taaagagatg atccgccacg
      961 ctggcaacag cggcacacgc gaagtggttc tcgggatggc gcaccgtggt cgtctgaacg
     1021 tgctggtgaa cgtgctgggt aaaaaaccgc aagacttgtt cgacgagttt gccggtaaac
     1081 ataaagaaca cctcggcacg ggcgacgtga aataccacat gggcttctcg tctgacttcc
     1141 agaccgatgg cggcctggtg cacctggcgc tggcgtttaa cccgtctcac cttgagattg
     1201 taagcccggt cgttatcggt tctgttcgtg cccgtctgga cagacttgat gagccgagca
     1261 gcaacaaagt gctgccaatc accattcatg gtgacgccgc agtgaccggg cagggcgtag
     1321 ttcaggaaac cctgaacatg tcgaaagcgc gtggttatga agttggcggt acggtacgta
     1381 tcgttatcaa caaccaggtt ggcttcacca cctctaaccc gctggatgcc cgttcgacgc
     1441 cgtactgtac tgatatcggt aagatggttc aggcaccgat tttccacgtt aatgcggatg
     1501 atccggaagc cgttgccttt gttacccgtc tggcgctcga tttccgtaac acctttaaac
     1561 gtgatgtctt catcgacctg gtatgctacc gccgtcacgg ccacaacgaa gccgacgagc
     1621 cgagcgcaac ccagccgctg atgtatcaga aaatcaaaaa acatccgacg ccgcgcaaaa
     1681 tctacgctga caagctggag caggaaaaag tcgcgacgct ggaagatgcc accgagatgg
     1741 ttaacctgta ccgcgatgcg ctggatgctg gcgattgcgt tgtagcagag tggcgtccga
     1801 tgaacatgca ctctttcacc tggtcgccgt acctcaacca cgaatgggac gaagagtacc
     1861 cgaacaaagt tgagatgaag cgcctgcagg aactggctaa acgcatcagc acggtgccgg
     1921 aagcagttga aatgcagtct cgcgttgcca agatttatgg cgatcgccag gcgatggcag
     1981 ccggtgagaa actgttcgac tggggcggcg cggaaaacct cgcttacgcc acgttggttg
     2041 acgaaggcat tccggttcgc ctgtcgggtg aagactccgg tcgcggtacc ttcttccacc
     2101 gccacgcggt gatccacaac cagtctaacg gttccactta cacgccgctg caacatatcc
     2161 ataacggcca gggcgcgttc cgcgtctggg actctgtact gtctgaagaa gccgtactgg
     2221 cgtttgaata cggttatgcc accgcagaac cacgcaccct gaccatctgg gaagcacagt
     2281 tcggtgactt cgccaacggt gcacaggtgg ttatcgacca gttcatctcc tctggcgaac
     2341 agaaatgggg ccggatgtgt ggcctggtga tgttgctgcc gcacggttac gaagggcagg
     2401 ggccggagca ctcctccgcg cgtctggaac gttatctgca actttgtgct gagcaaaaca
     2461 tgcaggtttg cgtaccttct accccggcac aggtttacca catgctgcgt cgtcaggcgt
     2521 tgcgcgggat gcgtcgtcca ctggtcgtga tgtcgccgaa atccctgctg cgtcatccgc
     2581 tggcggtatc cagcctcgaa gaactggcga acggcacctt cctgccagcc atcggtgaaa
     2641 tcgacgagct tgatccgaag ggcgtgaagc gcgtagtgat gtgttctggt aaggtttatt
     2701 acgacctgct ggaacaacgt cgtaagaaca atcaacacga tgtcgccatt gtgcgtatcg
     2761 agcaactcta cccgttcccg cataaagcga tgcaggaagt gttgcagcag tttgctcacg
     2821 tcaaggattt tgtctggtgc caggaagagc cgctcaacca gggcgcatgg tactgcagcc
     2881 agcatcattt ccgtgaagtg attccgtttg gggcttctct gcgttatgca ggccgcccag
     2941 cctccgcctc tccggcggta gggtatatgt ccgttcacca gaaacagcaa caagatctgg
     3001 ttaatgacgc gctgaacgtc gaataaataa aggatacaca atgagtagcg tagatattct
     3061 ggtccctgac ctgcctgaat ccgtagccga tgccaccgtc gcaacctggc ataaaaaacc
     3121 cggcgacgca gtcgtacgtg atgaagtgct ggtagaaatc gaaactgaca aagtggtact
     3181 ggaagtaccg gcatcagcag acggcattct ggatgcggtt ctggaagatg aaggtacaac
     3241 ggtaacgtct cgtcagatcc ttggtcgcct gcgtgaaggc aacagcgcgg gtaaagaaac
     3301 cagcgccaaa tctgaagaga aagcgtccac tccggcgcaa cgccagcagg cgtctctgga
     3361 agagcaaaac aacgatgcgt taagcccggc gatccgtcgc ctgctggctg aacataacct
     3421 cgacgccagc gctattaaag gcacgggtgt gggtggtcgt ctgacccgtg aagatgtgga
     3481 aaaacatctg gcgaaagccc cggcgaaaga gtctgctccg gcagcgcctg ctccggcggc
     3541 gcaaccggcc ctggctgcac gtagcgaaaa acgtgtgccg atgactcgcc tgcgtaagcg
     3601 tgtggcagag cgtctgctgg aagcgaaaaa ctccaccgcc atgctgacca cgttcaacga
     3661 agtcaacatg aagccgatta tggatctgcg taagcagtac ggtgaagcgt ttgaaaaacg
     3721 ccacggcatc cgtctgggct ttatgtcctt ctacgtgaaa gcggtggttg aagccctgaa
     3781 acgttacccg gaagtgaatg cgtctatcga cggtgatgat gtggtctacc acaactattt
     3841 cgacgtcagt atggcggttt ctacgccgcg cggtctggtg acgccggttc tgcgtgatgt
     3901 cgataccctc ggcatggcag acatcgagaa gaaaatcaaa gagctggctg ttaaaggccg
     3961 tgacggcaag ctgacggttg aagatctgac cggtggtaac ttcaccatca ccaacggtgg
     4021 tgtgttcggt tccctgatgt caacgcctat catcaaccca ccgcagagcg caattctggg
     4081 tatgcacgct atcaaagatc gtccgatggc agtgaatggt caggttgaga tcctgccgat
     4141 gatgtacctg gcgctgtcct acgatcaccg tttgatcgat ggtcgcgaat ccgtgggctt
     4201 ccttgtaacg atcaaagagt tgctggaaga tccgacgcgt ctgctgctgg acgtttagta
     4261 gtttaagctt cacctgcact gtagaccgac gtggataggg tgtcggtcta cggtttaaaa
     4321 gataacgatt actgaaggat ggacagaaca catgaactta catgaatatc aggcaaaaca
     4381 actttttgcc cgctatggct taccagcacc ggtgggttat gcctgtacta ctccgcgcga
     4441 agcagaagaa gccgcttcaa aaatcggtgc cggtccgtgg gtagtgaaat gtcaggttca
     4501 cgctggtggc cgcggtaaag cgggcggtgt gaaagttgta aacagcaaag aagacatccg
     4561 tgcttttgca gaaaactggc tgggcaagcg tctggtaacg tatcaaacag atgctaatgg
     4621 ccaaccggtt aaccagattc tggttgaagc agcgaccgat atcgctaaag agctgtatct
     4681 cggtgccgtt gttgaccgta gttcccgtcg cgtggtcttt atggcctcca ccgaaggcgg
     4741 cgtggaaatc gaaaaagtgg cggaagaaac tccgcacctg atccacaaag ttgcgcttga
     4801 tccgctgact ggcccgatgc cgtatcaggg acgcgaactg gcgtttaaac tgggtctgga
     4861 aggtaaactg gttcagcagt tcaccaaaat cttcatgggc ctggcgacca ttttcctgga
     4921 gcgcgacctt gcgcttatcg aaatcaaccc gctggttatt accaaacagg gcgatctgat
     4981 ttgcctcgac ggcaaactgg gcgctgatgg caacgcactg ttccgccagc ctgatctgcg
     5041 tgaaatgcgt gaccagtcgc aggaagatcc gcgtgaagca caggctgcac agtgggaact
     5101 gaactacgtt gcgctggacg gtaacatcgg ttgtatggtt aacggcgcag gtctggcgat
     5161 gggtacgatg gacatcgtta aactgcacgg cggcgaaccg gctaacttcc tggacgttgg
     5221 cggcggcgca accaaagaac gtgtaaccga agcgttcaaa atcatcctct ctgacgacaa
     5281 agtgaaagcc gttctggtta acatcttcgg cggtatcgtt cgttgcgacc tgatcgctga
     5341 cggtatcatc ggcgcggtag cagaagtggg tgttaacgta ccggtcgtgg tacgtctgga
     5401 aggtaacaac gccgaactcg gcgcgaagaa actggctgac agcggcctga atattattgc
     5461 agcaaaaggt ctgacggatg cagctcagca ggttgttgcc gcagtggagg ggaaataatg
     5521 tccattttaa ttgataaaaa caccaaggtt atctgccagg gctttaccgg tagccagggg
     5581 actttccact cagagcaggc cattgcatac ggcactaaaa tggttggcgg cgtaacccca
     5641 ggtaaaggcg gtactaccca cctcggcctg ccagtgttca acaccgtgcg tgaagctgtt
     5701 gctgctactg gcgctaccgc ctctgttatc tacgtaccag caccgttctg caaagactcc
     5761 attctggaag ctatcgatgc aggcatcaaa ctgattatca ccatcactga aggcatcccg
     5821 acgctggata tgctgacggt gaaagtgaaa ctggatgaag caggcgttcg tatgatcggc
     5881 ccgaactgcc caggcgttat caccccgggg gaatgcaaaa tcggtatcca gccgggtcac
     5941 attcacaaac cgggtaaagt gggtatcgtt tcccgttccg gtacgctgac ctatgaagcg
     6001 gttaaacaga ccacggatta cggtttcggt cagtcgacct gcgttggtat cggcggcgac
     6061 ccgatccctg gttctaactt tatcgacatc ctcgaaatgt tcgaaaaaga tccgcagacc
     6121 gaagcgatcg tgatgatcgg tgagatcggc ggtagcgctg aagaagaagc agcggcgtac
     6181 atcaaggagc acgttaccaa gccagttgtg ggttacatcg ctggtgtgac tgcgccgaaa
     6241 ggcaaacgta tgggtcacgc aggtgcgatc attgccggtg gtaaagggac tgcggatgag
     6301 aaattcgctg ctctggaagc cgcaggcgtg aaaaccgttc gcagcctggc ggatatcggt
     6361 gaagcactga aaactgttct gaaataaata tctgtaataa gaaatagccc tcgccgcttc
     6421 cctctacagg aggggcgaag ggctgtcggt ttcgacatgg ttggccatct tatgatggcc
     6481 ttttttattc gggcactaat ctgaaaagtt tgcccactgc ttttctccca tcttctgcgc
     6541 aataatgccc ggcaaaatat cccctctccg ttctgtaaaa gcatttatac aagaagaaaa
     6601 ttgttctgcc ggttgctctg gccctgatgg gtgtaaattc ggacgtttat gcggccgatg
     6661 taaatatcga tattttaagt gcgacagtga aagataaacg cattgaggga gtatcggtga
     6721 cgctgcaacg caatggcgca caatctgttt ctggaaccac aaatgcatcg ggcagcgtta
     6781 atctgggttc tacatttgct gatgatcaag atgctttatt gatcgtcaaa aaagagggct
     6841 attctaatct ggtggtcaaa tgttcgtgtg ctggcatgac ttatgcgatt agtccggcaa
     6901 tgactagtct ggatggcatg cgtgtagttt tgagctgggg cgaaaagcct tttgatcttg
     6961 attcacattt gatcttctct ggcggacata tctattttga tagtaaagag gggactgatg
     7021 ctaatctgga tgttgatgat acggatagct atggaccaga aactgtcact atcagcaaaa
     7081 agcattttgg cgaaagttac atctatgccg tgcaggacta tagcaataaa gggttgccca
     7141 acagtaatta tctctctgcc agtaaggcaa aagtgtttgt ttatgtaggg agctccctgg
     7201 tgcgtagtta tagtgtccct gctggaaaac gagggaacat ctggaccgtg tttaaactca
     7261 atccaaacgg cgagtttgaa gatatcaact ctgttaccag cgccaatttt aacgacacca
     7321 cattagacgt cagggattta gccactgtga taatgcctgc gacggactct tcagctccgg
     7381 cttcgcctgc gatgcaaaac agtggagata ctcaactggc aagaaaatat aatcgcgaag
     7441 gtgaagctgt ttataaaacc ggtcaacttg agcaagccat tcaactcttc caacaggcaa
     7501 cagaattaga cggcaattac ggacaggcat ttagtaacct ggggctggcg tatcagaaaa
     7561 atggcaacat cgcggaagca atttgggcaa atcgtaaagc gatctcgtta gccagtggag
     7621 caaatgcggc gacaacgcgc gctaactcct attataacat tgccaaaatt tatgaaactg
     7681 ctggacagga cgctgatgca ttgcagcatt atcaactcgc tcttcacgat gccattt
//
LOCUS       DAZFFE010000160         7682 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFFE010000160 DAZFFE010000000
VERSION     DAZFFE010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.161"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            5..223
                     /gene="yiiE"
                     /locus_tag="ACHJ69_004423"
     CDS             5..223
                     /gene="yiiE"
                     /locus_tag="ACHJ69_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO5017270.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPMRKAKGGRQRDEAVGVEELCKQHKE"
     gene            complement(447..1376)
                     /gene="fdhE"
                     /locus_tag="ACHJ69_004424"
     CDS             complement(447..1376)
                     /gene="fdhE"
                     /locus_tag="ACHJ69_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO5017271.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIVHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1373..2008)
                     /gene="fdoI"
                     /locus_tag="ACHJ69_004425"
     CDS             complement(1373..2008)
                     /gene="fdoI"
                     /locus_tag="ACHJ69_004425"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO5017272.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2005..2907)
                     /gene="fdoH"
                     /locus_tag="ACHJ69_004426"
     CDS             complement(2005..2907)
                     /gene="fdoH"
                     /locus_tag="ACHJ69_004426"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO5017273.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2920..5970)
                     /gene="fdnG"
                     /locus_tag="ACHJ69_004427"
     CDS             complement(2920..5970)
                     /gene="fdnG"
                     /locus_tag="ACHJ69_004427"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5383..5385),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO5017274.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSTWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLAANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6164..6997
                     /gene="fdhD"
                     /locus_tag="ACHJ69_004428"
     CDS             6164..6997
                     /gene="fdhD"
                     /locus_tag="ACHJ69_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO5017275.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQMFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7085..7573
                     /locus_tag="ACHJ69_004429"
                     /pseudo
     CDS             7085..7573
                     /locus_tag="ACHJ69_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
BASE COUNT         1773 a         1994 c         2043 g         1872 t
ORIGIN      
        1 ggtaatggcg atgaatacgg tttttcttca tttatcagaa gaggcaatta aacggctgaa
       61 taaactcagg ggctggcgta aggtttcacg atctgcaatt ttacgcgaag cggtagagca
      121 atatctggag cggcagcaat ttccgatgcg taaggcaaaa ggcggcaggc aaagggacga
      181 ggcagttggt gttgaagaac tatgtaagca gcataaggaa tgacaaaaag attttttcat
      241 tcttgaatat aaaaacaaaa aaccagatga ccttattctg gtattaatac aaggttgttt
      301 tacttgaact tataataact gcaactgtta catcatatct ggaaaacgcc tcgcgaaata
      361 cgagagattt tgcgggaata gcaggtgtcg tcacacccta tgagctgtaa tccgggcgac
      421 cagcgccacc cggaaaacgt cagagattac tccccttcac ccggaaacag gaacgggttg
      481 atggaactgc gggcatagcc ttcttgctcc attcgcgcgt ccagtaccag agaggcgagg
      541 tcatctgcca cggcttcaac tttcggttct ttttcctgat aaagaatctt caggtaagtg
      601 ccgcagtcat cgcagctttc ggctttaatc gcggcctgtt cgtcatccag cgaccagtaa
      661 tgcagtttgc cgctctgttc acagttgctg cattttacgc gtactacgtg ccattcagtt
      721 tcacacaggt tgcagtgcag gtaacgcaga ccctgagtgg tgccaatttg caccatgctg
      781 gacaccggca tagagccaca taccgggcaa tattgacgtt gttcgccgta ttcagcgcgg
      841 gctttgccgg ggatcagatt agccatctgc gcccaataga gcgacagcgc agcccagata
      901 aacggcgctt tatcgctgct gacggacgag aaatcagagg caaacagtgc gctggccata
      961 tcttccagct cctgagtcga tgccttctcc agattctcaa tcactgccag cgccgggccg
     1021 ctcatttcag gtttcagctc agcaatcagc gccatcagca gcttttgcca gtgcttatca
     1081 cgcggcagaa cgtgaatatc cagcgggggc ttgccctgtg cgctggcttc tttaatgcgc
     1141 gtggtcagat ccatctccag cggatggtcg tacagcacca cttcctgggc gtggacgata
     1201 agcgcagcaa agcgcaggta atcacccagc ggattatttt ctgccagctc gcgcagacgc
     1261 tcggcgcggc ggttgtataa attcttgagc cgagggaaca ataacggcgg aatcatatcc
     1321 gccgtacgtt tctcgctcga acccagctca tcttgcggga ttatgcgaat actcattcag
     1381 ctttcttttc cgttgtcttg cggacctcac ggtaccagcg cgggtgatgt ttcttcgccc
     1441 aggcgctggt cacccatcct tccaccatgg cggtaatcgt gcctttcacc caaagggcgg
     1501 cgtagatatg caccatgata accacaatta acgccactgc ggcaaatgaa tgcagcatta
     1561 acgcgaatcg gatcaccggg attgagaaag caggcgcaaa ataaggacgc cagataatca
     1621 cgccgctcac cagcagcaga accaggaaaa taatcgccgc ccagaaaacg catttctgac
     1681 cgaagttata acgcccggtg tcacctactt cctcgttgac gacgatctta cgaatattct
     1741 tcgcccaaaa gatatcatcc cgattgatta ggttgtgatg ccagtaacgg aaaaacatga
     1801 tgatgaacga ggcaaacata accacgccga caaacgggtg cagaatgcgc gccagctgcg
     1861 gtgtgcccat gatttgcatc aaccagttga aggacgggaa caaaaagccc agcccgctca
     1921 ccgccgccag gatgaagcag aaggcggtga tccagtggtt gatacgttcc ggcgcggtgt
     1981 agcgcacgat ggtgtcacgt cgtttcattt gcgctcctcg tctttctctt cgtgcagatt
     2041 attctcttcc tcatccgcac ggttcggacc gacacccacg tagtggaaga tactggctgc
     2101 gaaggtagcc gcaaagccaa cagctgcgag cggtttccag atgcctttcc agaatttcac
     2161 ggtttcgctg atttccgggt tctccggcaa gccatgatac agatttggct tgtcagcatg
     2221 gtgcagcacg tacataacat gtgtaccacc gacgcctgcc gggtcgtaca aacccgcatt
     2281 gtcgtaacca cgggttttca gctccgccac gcgctcgctc gccagcgttt tcatcgactc
     2341 tttcgtaccg aagtgaatcg cgcccgttgg gcaggtcttc acgcaggccg gttcttgccc
     2401 aaccaccacg cggtcaacgc acaacgtaca tttgtagacg cggttgtctt ccgggttgag
     2461 gcgcggaatg tcgaacggac agcccgcaat gcaataaccg cagccgatgc actgctcgga
     2521 ctggaagtcg acgataccgt tggcatactg aataattgcc ccttccgccg ggcacgcttt
     2581 caggcagcct ggatcggaac agtgcataca gccgtctttg cggatcagcc attccagttt
     2641 gtcgttctgc tccacttccg agaatcgcat caccgtccac gatttggcac ttaaatcatt
     2701 ggggttgtcg tacaccccaa tgttattgcc gacggtatcg cgaatgtcgt tccactctga
     2761 acacgccacc tgacaggctt tacagccgat acaggtggta acgtcgatga gtttcgccac
     2821 ttcttcctgg aagtcccgcg cctgaggcgc gggggtcaga ccgttagtcg cggaacgacg
     2881 aatgatatct tgcgattgat aagccatatg tcgtctccgt tacacctttt ccacattcac
     2941 aaggaaggac ttaaactccg gcgtctgcgt gttcgcatca ccgacgaatg gcgtcaacgt
     3001 attggcaata aagccttttt tcgcaacacc ttcatagccc cagtgaatcg gaataccgat
     3061 ggtatcgata tctttgccgt ttgctttcag cgtgcgaata cgtttggtca ctaccgcttt
     3121 ggctttgata tagccacggt tggaggagac tttcacagta tcgccctggg caatgccaag
     3181 tttattcgcc agcgactccc cgatttccac aaactgctct ggttgcaaaa ttgcgttcaa
     3241 cagcgcgtgt ttggtccagt agtggaagtg ctcggtcaga cgataggtgg ttccgacata
     3301 cgggaactta tcggctttac ccaatgcttc ggcgtcgtct ttaaagatac gcgcagccgg
     3361 gttcgagata acgtttggat gcagcgggtt agttcccagc ggcgtttcaa acggctcgta
     3421 gtgttccggg aacggacctt ccgccatctt atcgagggca aacagacgtc ccatgccttc
     3481 ctgctgcatg ataaacggcc ctacgccgct gcccggaggc gctgcgctgt agtccggaat
     3541 atcccagccg gtccacttag tgccgtccca tttcagcaac tgacgcttcg gatcccacgg
     3601 gttaccctgc ggatctgcgg aggcgcggtt atacagaatg cggcggttaa gcggccatgc
     3661 ccatgcccag cccagcgtgt taccgaggcc agacggatcg gcgttatcac ggcgtgccat
     3721 ctggttacct tctggcgtcc agctaccggc gaaaatccag cagccacagg aggtggtacc
     3781 gtcatcgcgc agttgggcga acgagctaag ttgttggcct ttcttgacga taaccgcacc
     3841 ggttgccgga tcggtaatat cggccagcgc cttaccgttg ctctccatcg ccacttcttc
     3901 tgacttcggc tcatgcggga tggcgtagtt ccaggtcatg ttcagcacct ggtccgggtt
     3961 cgcgccaccc tgttcggcat acatcttgcg caagcgcagg aagataccgg agaggatctc
     4021 gccgtcagtc agcgcaatcc caggggcgtc cgcacctttc cagtgccact gcaaccagcg
     4081 acctgagtta acgattgaac cgttctcttc cgcaaagcag gtcgatggca gacggaacac
     4141 ttcggtctgg atcttcgacg agtcaacttc gttcagctca ccgtggttct gccagaagtt
     4201 agaggtttca gtgttcagcg ggtcgatagt cacgaggaat ttcagcttcg acagacagcc
     4261 gatcactttg tttttgttcg ggaatgaggc aacagggtta aagccctggc agatatagcc
     4321 attgaccttg ccctctttca tcatctcgaa gtattgcagg acgtcgtagc ctttatccca
     4381 cttcggcaac cagtcaaagc cccagctatt ttccgccgtc gctttatcac caaagaatgc
     4441 cttcatcatt gagacgaaga atttcgggta gttgccccag tagttgacct ggccttccag
     4501 cagtggtttt ggcgtgttgg cagcgaggta agtttgcaga tcggtctgct tctcgcttgg
     4561 cagcgtcatg taacctggca gactctgcga cagtagcccc aggtccgtca gcccctgaat
     4621 attggagtga ccgcgcaggg cgttaacgcc gccgccagcc atccccatgt tgccgagcag
     4681 cagctggatc atcgccatcg tacgaatgtt ttgcgcacca atggagtgtt gcgtccagcc
     4741 gagggcgtag aggaacgacg ctgttttatc gtgagcactg gtttcggcga tgtattcgca
     4801 gactttcagg aacgcgtctt ttggcgtacc gcagatgttt tcaaccacat ctggcgtata
     4861 acgggaaacg tgctgtttca gcaagttcca cacgcaacgt ggatgttgca gggtagtatc
     4921 acgtttggcg aagccgtttt catccagttc gtaagtccag gtggacttat cgtacttgcg
     4981 tttttccgcg tcgtagccgg tgaacaggcc atcttcaaag ccgtaatcct cacgcacgat
     5041 caggctggcg ttggtatagg cttcggtgta ttcgcggttg aatttttcat tgttcagcag
     5101 gtacagcaat acgcctgaca ggaaagcaat gtcagtaccg gaacggatag gggcatagta
     5161 gtcagccacc gccgctgtac gtgtaaagcg aggatcgatc acaatcagct tcgcgccgtt
     5221 gtgaattttg gcttccatcg cccagcggaa cccgaccggg tgagcttcag cggcgttacc
     5281 gcccatcacc acgacgaggt tggcattctt gatgtcgacc cagtggttgg tcatcgcacc
     5341 gcgaccaaat gttggagcaa gacttgctac cgttggtccg tgtcagacac gcgcctggtt
     5401 gtcgaccgcg agcataccca gcgcgcggga gaatttttgc gttaaatagc cggtttcgtt
     5461 actcgacgcg gaagcacaca gcatcccggt ggagagccag cggttaacag tcacgccttc
     5521 ggcgttttgc gcaatgtagt tagcatcgcg gtcttctttc atcagtttgg cgatgcgatc
     5581 aaacgcctct tcccaactga tttgttgcca tttatcagaa cctggcgcac ggtattccgg
     5641 aaacttcaga cggctttcgg agtggatgaa atccaccagg ccagcgcctt tcggacaaag
     5701 tgctccgcgg ttgactgggt gatccgggtc accttcgata tggaagatag atgctttggc
     5761 gttttttgct ccgtcaccga ggctgtacat caacagccca cagcctacgg aacaataggt
     5821 gcaggtatta cgggtttcgc gggtgcgcag cagtttatac tgccgggttt ccgcgagtgc
     5881 tacgctgggt gcaaaaccca gtgccgctgc cgtggtgcct gccataccgc cagcgcagat
     5941 cttaaagaac tgccttctgc tgacctgcat ggattgctcc ttgtttcgac attgtcacgt
     6001 cccatttaca ttcgcttgct gcgtgtgcag ggagtgggag ttatttttct ttgcggaagg
     6061 ggccgcaaag gtccagaatt ggctcaattt ccctccatcc aggaaggatt tgtaacagaa
     6121 taccataatg ttggtgtgtg tgttcttatc tggttaatag aaagtgaaaa aaacacagca
     6181 aaaagaaatc gagaatgtga caaatatcac aggtgttcgt caaattgagt tatggcggcg
     6241 tgatgattta caacacccac ggttagatga ggtggcggaa gaagttcccg ttgcgctggt
     6301 ctacaacggc atttcgcatg tggtgatgat ggcgtcgccc aaagaccttg agtactttgc
     6361 gctcggtttt tcgctttccg aaggaattat cgaaagcccg cgcgatatct tcggcatgga
     6421 tgtcgttcct tcctgtaatg gcctggaagt acaaattgag ctttccagcc gccgctttat
     6481 ggggttgaag gagcgccgcc gggcgctggc gggacgtacg ggctgtggcg tatgcggtgt
     6541 agaacagctt aacgatatcg gtaaaccggt gcagccgctg ccgttcaccc agatgtttga
     6601 tctcaacaaa ctggatgatg cattacgtca tctcaatgat ttccagccag tggggcaact
     6661 gactggttgt actcacgccg ctgcatggat gttgccatct ggcgaactgg tcggcgggca
     6721 tgaagacgtg ggtcgccatg tggcgctgga caaactgtta ggccgccggt cacaagaagg
     6781 ggaaagctgg cagcaaggtg cggtgctggt ttccagccgt gccagttatg aaatggtgca
     6841 aaagtcggcg atgtgcggcg tagagatttt gtttgcggtg tctgccgcga ccacacttgc
     6901 tgtagaagtg gctgagcgct gtaatctgac gctggtgggc ttttgtaaac cgggtcgggc
     6961 aacggtttat acccatccgc agcgtttgag caattaatta aaataagtcc tgcgatatat
     7021 attaaattac aggacttatt catttcgtga attttgttat tttttcataa acaaaacgcg
     7081 tgtagtggcg ttaacgcctg cgctgcaacc tattgatggc gtggcggtga gttatattga
     7141 tgctgccgta gcgttgggta ataccattaa cgaaatggat aaatattaca cccaggaaaa
     7201 ttataaagac gatgcgtttg cgaaagggaa aacattccac tactactctc tggcagtcat
     7261 gatttcagcg aaacaaatta ataacctgat atcgcaagat aagttagatg cagaagcggc
     7321 aatgaagaaa gtgtctgaac tggaaacgct ggtggcgcaa gccaaagaag cggataaaag
     7381 cggcatgaat ttctcgttta ttaattcggc aggccagtat caactcgagg ctaaaaaata
     7441 cgttcgccgc atcagagata aagtcccgta ctctgactgg gataaagagc aacttcagga
     7501 cgcaaactca agctggatgg ccgaagactc atttccacga gcgttatgcg attacaacga
     7561 aatggtcgat gaaatatttc agttaattgt aatcgcgggg cgggtttgtg atgagcacgg
     7621 atatgtcacg aagtcggtaa tgactccaac ttattgatag tgttttatgt tcagataatg
     7681 cc
//
LOCUS       DAZFFE010000161         7540 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFFE010000161 DAZFFE010000000
VERSION     DAZFFE010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.162"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..173
                     /locus_tag="ACHJ69_004430"
     CDS             <1..173
                     /locus_tag="ACHJ69_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HFO5017276.1"
                     /translation="EFTLRLSILTSEQPVLVLRIVRVEAAEEEIAKEFRDLLKERFEE
                     EDILTFIGTFSV"
     gene            250..726
                     /locus_tag="ACHJ69_004431"
     CDS             250..726
                     /locus_tag="ACHJ69_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017277.1"
                     /translation="MQFKDLPEDIQKIAADTLKDHLSVINLTKEPKANLENISRNMRD
                     VFVGMYAYDNEKHEEHIQNCRLNKGQQNIKHNITENDKSINSVERVNNSQKTDPLVFW
                     NIVQAFALSKVYEFNNINHSGIDTHQSSHREGVSMLLSLLREQGECPHSTLNDTEQ"
     gene            883..1008
                     /locus_tag="ACHJ69_004432"
                     /pseudo
     CDS             883..1008
                     /locus_tag="ACHJ69_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Eaa protein"
     gene            1084..1497
                     /locus_tag="ACHJ69_004433"
     CDS             1084..1497
                     /locus_tag="ACHJ69_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO5017278.1"
                     /translation="MEPGNVPLYIGLPRIEPASQTAKRSFQEWLSEQKEKIDVDCGCV
                     SIETLTHWMKSAYEAGNSPVTPDGWISCSERIPDDKQYVWCWGKSYGWTECDTFEGYY
                     DWSRNKWWAVTDDGEEPASKVTHWMPLPEPPQEVN"
     gene            1497..1782
                     /locus_tag="ACHJ69_004434"
                     /pseudo
     CDS             1497..1782
                     /locus_tag="ACHJ69_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241156.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
     gene            1878..2147
                     /locus_tag="ACHJ69_004435"
                     /pseudo
     CDS             1878..2147
                     /locus_tag="ACHJ69_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724814.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2177..2959
                     /locus_tag="ACHJ69_004436"
     CDS             2177..2959
                     /locus_tag="ACHJ69_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFO5017279.1"
                     /translation="MKSIIKHFECEISQGNIVGLDAAFYQGKPVFLAAPFAKALGYAN
                     TADALKKHCKSLIKLNYRESRELGLGDNPRGIQLIGQGDAFRLIMRSSLPSAERVQDW
                     VCEVVLPALMETGTYSLKQEKSTPSNGLPEYRKAKALKMEMEVISSVLDRLPHLGDKA
                     KQAAYASVINRSAGFNVIPLPVLDEHHYSATEVGKQFGVTANKIGRIANTYMLKTEQY
                     GKWFIDKSPYSDKQVESFRYNNRGVRKIEEILEAENKAEFGT"
     gene            complement(3002..3307)
                     /locus_tag="ACHJ69_004437"
     CDS             complement(3002..3307)
                     /locus_tag="ACHJ69_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO5017280.1"
                     /translation="MAMFNPPHPGEIIADILEDQNIGIRELARALDVAPSTVQRLVSG
                     NATISPEMAVRLAAVLGGTPSSWIRLQTAWSLEKAEREVDVSHLSTKYRPAEISPHA"
     gene            complement(3307..3585)
                     /locus_tag="ACHJ69_004438"
     CDS             complement(3307..3585)
                     /locus_tag="ACHJ69_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005188495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5017281.1"
                     /translation="MIKSWKHKGLQRFFETGNASGINPNHVKRLRTRLQVIDQAEIID
                     DINLAGYRLHQLQGGRSDTWSVTVSGNWRITFEFRNGDAYILNYEDYH"
     gene            3929..4240
                     /locus_tag="ACHJ69_004439"
                     /pseudo
     CDS             3929..4240
                     /locus_tag="ACHJ69_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232082.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            4509..5177
                     /locus_tag="ACHJ69_004440"
     CDS             4509..5177
                     /locus_tag="ACHJ69_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO5017282.1"
                     /translation="MTMQLAVESLVVVTYSNVPVITTGLLAHLYKTEEKHIRQNFKRN
                     ECRFIAGKHFFKVSGCELDSLRTSQRGLQISPKTRSLILWTERGAARHAKMLETDRAW
                     EVFEKLEDCYFSQKIPEQLQLPESTLSINYPLSWFSEHHPYSMMSYVDRKTLNLDVSV
                     LFDMPSPTMRILNELHSKGYNVDAAVAEFNAFKHLTEEMRRTLQDISRLSDRNSRKGF
                     SLSL"
     gene            5228..5659
                     /locus_tag="ACHJ69_004441"
     CDS             5228..5659
                     /locus_tag="ACHJ69_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFO5017283.1"
                     /translation="MKQIAFYRRSGRPGVFRGLKERVTWMIQSRGRPVTGSEIAEKFG
                     VSLCEFNKVARSLTKGSKVVKIKASESFTTDTGIVDRFFSLESNPRRDTPRSRNAVPP
                     FSRRSREHAAKNCREEYEQKAKRRRRLIKAGLYIDEFEKAL"
     gene            5656..6282
                     /locus_tag="ACHJ69_004442"
     CDS             5656..6282
                     /locus_tag="ACHJ69_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFO5017284.1"
                     /translation="MTNKYTLIYAAPPWTFRDKATDGQRGASFKYPVMSLLDICRLPV
                     WELAADNCLLAMWWVPTQPLEALKVVEAWGFRLVTMKGLTWNKCGKRQTDKLVMGMGS
                     TTRANSEDCLFAVKGNLPERINAGIIQSFTAPRLDHSRKPDMAREKLVQLLGDVPRIE
                     LFARHTSHGFDVWGNQCGTPSIEMVPGIVKFLEKTNERKNDVDKGITS"
     gene            6242..6454
                     /locus_tag="ACHJ69_004443"
     CDS             6242..6454
                     /locus_tag="ACHJ69_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO5017285.1"
                     /translation="MSEKTTLTKASPVELRQCLEIANQLARSGIRFVPIPITADAELH
                     LFGEILSRKLDELEKLVEEADTSPIA"
     gene            6490..6876
                     /locus_tag="ACHJ69_004444"
     CDS             6490..6876
                     /locus_tag="ACHJ69_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO5017286.1"
                     /translation="MNAVLTELNKLGKATADSISKGLNIDLNDVIDTLWKLKNQGIVK
                     VKNGIWQAVEGVGSKTDIDSVQPVQPVQPVQPVQPVQHNIIGDLLRKSRKEARRAGRK
                     QKRWEGACKALQELNKYRDLINELSE"
     gene            6955..7137
                     /locus_tag="ACHJ69_004445"
     CDS             6955..7137
                     /locus_tag="ACHJ69_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5017287.1"
                     /translation="MEVNLMTEKEVSDLLQKRRTALYNLRKKHGFPEPVLTHPARYSR
                     QAVEQWLKAGGINRAV"
     gene            complement(7121..7498)
                     /locus_tag="ACHJ69_004446"
                     /pseudo
     CDS             complement(7121..7498)
                     /locus_tag="ACHJ69_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         2178 a         1711 c         1775 g         1876 t
ORIGIN      
        1 gggaattcac gctgcgatta agcattctga caagcgaaca acctgttctg gtgctacgta
       61 tcgtgcgtgt tgaagctgcg gaagaagaaa ttgccaaaga gttccgtgac ctgctgaaag
      121 agcgtttcga agaagaagat atcttaacct tcatcggcac gttctcagtg tgatgaaatc
      181 tgtgacaggg acgtcacgac atatcgctca tatttcacga gaaaatacac agttcttgga
      241 gggtatacta tgcaatttaa agatttacca gaggatattc agaaaatagc tgcggatacg
      301 cttaaagacc atttatcggt gataaattta acaaaggaac caaaggcgaa tctggaaaat
      361 atatcccgta acatgcgaga tgtttttgtc gggatgtatg cttatgacaa tgaaaagcac
      421 gaggaacata ttcaaaattg ccgccttaat aaaggacaac aaaacatcaa acacaatata
      481 acagaaaacg acaaatcaat taattctgtt gaaagagtga ataattcgca gaaaacagac
      541 ccattggtat tctggaatat cgttcaggct tttgccttga gcaaggtgta tgagtttaac
      601 aacatcaatc attcagggat cgacactcat caatcatcac atcgtgaggg ggtgtccatg
      661 cttctgtctc tgttacgaga gcagggcgaa tgtcctcact cgacattgaa cgacactgag
      721 caatagtcca tccgtatttt ccgccaactt gatatcgtca tcaaacttaa ctttgatcgt
      781 gcgcttccag ttgtgaataa caaccttgct gtgtttagtc attggcggca tcagttgccg
      841 attgctgatg tccgcccttt ttaaagtatt aagggttaac tcatgacggc ttttaccgac
      901 aaagagctaa ttaaagaaat taaagaacgt atcagcagtc tggaggtgcg ggacaatatt
      961 gagcgccgtg tttatgagat tgcgttgact gcattgaccg ctgaaccatt cgccactatc
     1021 gacaccgtgg gaattgagct cgttaaatat ggttgtaaca cgtttatttg tcccgacaat
     1081 tcgatggagc cgggaaatgt accgctatat atcggcctgc cacgaattga gccagcaagc
     1141 cagactgcca agcggtcatt ccaggaatgg ttgtcagaac aaaaagaaaa aatagacgtt
     1201 gattgcggat gtgtaagcat cgaaacgctt acgcactgga tgaaatcagc ttatgaggct
     1261 ggcaactctc cggtaactcc ggatggttgg ataagctgta gtgagcgaat tccggacgat
     1321 aaacagtatg tttggtgttg gggtaagtct tacggctgga ctgagtgcga taccttcgaa
     1381 gggtattacg attggtcgag aaacaaatgg tgggcagtta ctgacgatgg ggaagaaccg
     1441 gcatcgaaag taacccactg gatgccgcta ccagaaccgc cgcaggaggt taattgatgg
     1501 actccttcgc gaaatatacg attattgact ggatagcatt ccttcaggtt ttgctcatct
     1561 ggttttatat ggcttacagg agtggacagt ggattgtcag tgtagcctgt agcaagggat
     1621 ggcgttggtg gaaccgaaag aataaaaaaa gcactggcct tggattcatt ttacgaagca
     1681 ttcaatctta acagccttca gcctggttct gtcgttgtag tcaccactca aagcggcatg
     1741 acgatacaaa ttcacaagcc aaaggaggaa ggtcgtggct aacctgcaac ttgccgtcaa
     1801 aggtgaataa gaatcctcgc actcgcgggg atttctttta tctgaactcg ctacggcgag
     1861 cttgagaggt taaaacgatg aaaaataatg taatgaaaaa tggaactatc gacagccagg
     1921 ctctgttgaa aatggtgaat gaagcgcgga gactgtgcgg tgaaaaagaa gtacgcaaca
     1981 acgacttcat tgcacgcatc aaagatgaac ttgatgggga gggttacgaa attttcgtaa
     2041 cccccatgga taagaaaaaa ggcggtgcgg atcaggttgt tattgtgatg acctataaac
     2101 aagcgcttcg cgtcgccgca cgtgagtcga aagccgtccg ccgttctttt gcgtaacccc
     2161 caacaggaga aatgatatga aatctattat caaacatttt gagtgcgaaa tctcacaggg
     2221 taatatcgtt gggctggatg cagctttcta tcagggtaag cctgtgtttc tggctgctcc
     2281 attcgcaaaa gccctgggat acgccaacac ggcggacgca ttgaaaaagc actgcaaatc
     2341 attgattaaa cttaattatc gcgaatcgcg agaattaggt ttaggtgaca atccgcgagg
     2401 aattcaactt attggccaag gtgacgcttt tcgtctcatc atgcgtagct cgttaccatc
     2461 ggcagaacgg gttcaggatt gggtttgtga agttgtcctc ccagccctga tggagacagg
     2521 aacttacagc cttaaacagg agaaaagtac accatcaaac ggccttcctg aataccgcaa
     2581 agctaaagcg ctgaaaatgg aaatggaggt tatctcaagc gttctggatc gtttaccgca
     2641 cctgggcgac aaggcgaaac aggctgctta cgcctcagtg attaaccgtt ctgctggttt
     2701 taatgttatc ccccttcctg ttcttgatga acatcactac agtgcaacag aggttggtaa
     2761 acagtttggc gtcactgcaa acaagatcgg tcgcattgcc aatacctaca tgctcaaaac
     2821 agaacaatac gggaaatggt tcatcgacaa atccccgtat tcggataagc aggtagaaag
     2881 ctttcgatac aataatcggg gagtacggaa gattgaagaa atcctggagg cggagaacaa
     2941 agcagagttc ggaacctgat atcctccact ctccgacgcc gccagaatga cggcgcggtg
     3001 gttaagcatg aggcgaaatt tctgccgggc ggtattttgt tgagagatga gatacgtcaa
     3061 cttctctttc cgctttttcg aggctccatg ccgtctggag acgaatccat gaagacggag
     3121 ttccacccag gacagcagcg agacgaacag ccatttcagg agatattgtc gcattacctg
     3181 aaaccagtcg ctgaacagtg gaaggcgcga catcaagcgc tcttgccagt tccctgattc
     3241 caatattctg atcttccaga atatcagcaa taatttctcc gggatgaggg gggttaaaca
     3301 tcgccattag tggtaatcct cgtagttaag aatataagca tcgccgttcc gaaactcgaa
     3361 tgtgattctc cagttaccgg agacggtgac agaccatgta tcggatctgc ccccctgcaa
     3421 ctggtgcagc ctgtacccgg caaggttaat atcatcaatg atttcagctt gatctattac
     3481 ctgaaggcgc gttctcaggc gtttaacgtg attgggattt atgcctgatg catttcctgt
     3541 ttcaaagaaa cgttgaagtc ctttgtgttt ccagcttttt atcatgcttg tttcctgttg
     3601 cgttacgcga atcattatag cgactgttgt gtaatgcgca acggttgttt gacaaaaata
     3661 ggtatccggg attatattca ccgcacgcca gcaaaatctg gcgtcgggat taggaacccc
     3721 ggatattcaa ccgcgacaga cacacgccgc gagcgtgttt tttattgtcg tatgcacacg
     3781 cacatctgaa ttatggtggg gcgtatgggg cagccgaaag gctggccggt tggttgatcc
     3841 ggtagttcct aaccctgtac gtctcgccac ccgatgatta ggaacctgac ggtggtgaat
     3901 gaaaaactca tcaaccggag gcgtcactat gacaactcaa gtttctgttg aaacactctc
     3961 cacgattact tacaagcaga tccccgttat cactactgaa cttttggcgc acctttacgg
     4021 cacagaagct atttgtattc gccagaatca ccacgaaaac aaaggtcgtt tcattgagga
     4081 aaaacacttc ttcaaacttg aaggtgaaac tttacgtgag ttcaagcaca gagtagcttt
     4141 taactactct gtgaaaattg cccgtaacgt tcgttccctc atcctctgga ctgaacgcgg
     4201 cgctgcccgt catgcaaaaa tgctcgaaac cgatcgggct tgacgttata ggtcgttact
     4261 tgtagattta tctttgtgag gcgtcagaac ctctctaaag cggacaaaac caaccccgtc
     4321 agtgttggat tttttatgcc tgttattcag tgatagcaca atgtgctgtc acatccccga
     4381 acaatgtcgg gagggcgacg aatacaacac ccgtaagggg aataagtccg cggttcttta
     4441 ggccgttctg aacctcccga caccactatc agaggtggta atttcagaaa aagctaaagg
     4501 aggtcgttat gaccatgcaa ctcgctgtag aatccctcgt cgtcgttact tactctaacg
     4561 tccctgtaat aacaacaggg ttattggcac atctatacaa aactgaagaa aaacatatac
     4621 ggcagaactt caaacgtaat gaatgtcgtt tcattgcagg aaaacacttc tttaaagtaa
     4681 gtggctgtga acttgatagt ttgcgaacct ctcaaagagg tttacaaatt tccccaaaaa
     4741 cccgctccct catcctctgg actgaacgcg gcgctgcccg tcatgcaaaa atgctcgaaa
     4801 ccgatcgggc gtgggaggtg ttcgaaaaac tggaagactg ctatttcagt caaaaaatcc
     4861 cagaacaact tcagcttcca gaatcaacgc tatctatcaa ctaccctcta tcgtggtttt
     4921 cagagcatca cccctactcc atgatgagct atgttgatcg aaaaactctt aacctggacg
     4981 tttccgtgct cttcgatatg ccaagcccaa ctatgcgcat cctcaacgag ctacacagca
     5041 agggctataa cgttgacgcc gctgttgctg aatttaacgc cttcaaacat ctgacggaag
     5101 aaatgcgccg tactctacag gatatttcaa gactgtcaga tcgaaattcc cgaaaaggct
     5161 tctcgttaag cctctgactt ccccaccccg acatcccgtc ggggttttca tatctggaga
     5221 caaaaatttg aaacagatcg ctttctacag gcgttctgga aggcccggtg tcttccgtgg
     5281 gttaaaagaa cgcgtcacct ggatgattca gtctcgtggt cgtcctgtta ctggcagtga
     5341 aatagcagag aaatttggtg tttcactttg cgagttcaac aaggtagctc gcagcctgac
     5401 aaaaggcagt aaggttgtga aaatcaaggc ttcagagtca ttcaccacag acaccggaat
     5461 cgttgatcgc tttttctccc tcgaatcaaa tcctcgtcgt gacacacctc gttcacgcaa
     5521 tgccgttcct ccattcagtc gcagaagccg tgaacacgca gcaaaaaact gccgcgagga
     5581 atacgagcaa aaggccaaac gccgtcgccg actgattaaa gcaggacttt acattgatga
     5641 gtttgaaaag gcgctatgac taacaaatac actctcattt acgcagctcc accttggaca
     5701 ttccgcgaca aagcaaccga tggtcaacgc ggtgcaagtt ttaaatatcc ggtcatgagc
     5761 cttctcgata tctgccgcct cccggtatgg gaactggcag cagataactg cctgttggct
     5821 atgtggtggg tgcctacaca accacttgaa gcattgaagg ttgtagaagc atggggcttt
     5881 cgcctggtga cgatgaaagg attaacctgg aacaaatgcg ggaaaagaca gaccgacaag
     5941 ctggtcatgg gtatgggtag caccactcgc gctaacagcg aagactgcct ttttgcagtg
     6001 aaaggaaatc tgcctgaacg cattaacgcc ggaataattc agtcatttac agcaccacgc
     6061 cttgatcact cccgcaagcc ggatatggcg cgagaaaaac ttgtgcaact tcttggcgat
     6121 gttccccgga tagaactgtt cgcccgccac acctcgcatg gatttgatgt atggggtaac
     6181 caatgcggaa caccatccat tgagatggtt ccgggtattg ttaaatttct ggagaaaacc
     6241 aatgagcgaa aaaacgacgt tgacaaaggc atcaccagtt gaattaaggc agtgtctgga
     6301 aatcgcaaat caacttgcca gaagtggaat acgctttgtt ccaatcccga tcacagcaga
     6361 tgcagaactt catctgtttg gtgaaattct ttcccgaaag ctggatgaac tggaaaagct
     6421 ggtagaagaa gctgacacct caccaatcgc ataacacccc caccgacatt aaaatatcag
     6481 gagaaaaaaa tgaacgccgt actcacagaa ttgaacaaat taggaaaagc aacagccgac
     6541 agcatttcta aaggtctcaa tattgatttg aatgacgtca ttgatacgct gtggaagttg
     6601 aaaaaccagg ggatcgtaaa ggtaaaaaac ggcatctggc aggcagtaga gggagttgga
     6661 agcaaaacag atattgattc agttcaacca gttcagccag ttcagccagt tcagccagtt
     6721 cagccagttc agcacaacat tataggtgac ctgctacgta aatcacggaa agaagcgcgc
     6781 cgcgccgggc ggaaacagaa acgatgggag ggtgcatgta aggcgttgca agaactgaat
     6841 aaataccgtg acttgatcaa cgaattgtca gagtgaacgt agcgggaatc cacgtccttt
     6901 agggcgtgga tgatatcagg ctgttgaaat acatgtagaa aaggagcgca tactatggaa
     6961 gtcaacttaa tgaccgaaaa agaggtttct gatttgttac aaaaaaggcg cacagctttg
     7021 tataatttgc gaaaaaaaca cggattcccg gaaccagtac ttactcatcc ggcgcgatat
     7081 agtcgtcagg ccgttgaaca atggcttaaa gccggaggaa ttaaccgagc tgtttagcgt
     7141 gccagaaaat tttatcagca tacaactcat acgcatcttt ctgttccgac aaccagtcgt
     7201 gtttattata cacggccata actcccccaa gttcatgccc cagcattttt tcagtgacgt
     7261 ggggcataat tccttcccca gataaattcg ttaccagaga acgcctaaaa tcatgcgttc
     7321 tccattcagg aatatcaatt ttctccctta actttttcat gtacaaatta gccgacgaac
     7381 gatcaatagg tttatctatt tcctgcccgg ggaatagcac atcaaaaccc gcattaagca
     7441 atctctcgat ataaggttta acctgagtga ataccggacg ccgggtgatg ctgccaactt
     7501 actgatttag tgtatgatgg tgtttttgag gtgctccagt
//
LOCUS       DAZFFE010000162         7452 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZFFE010000162 DAZFFE010000000
VERSION     DAZFFE010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.163"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..448)
                     /locus_tag="ACHJ69_004447"
     CDS             complement(<1..448)
                     /locus_tag="ACHJ69_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017288.1"
                     /translation="MVDIVEADKTDIYFIQESVYGKIGLPAFGNTIGPSAQQVVKKVF
                     AVVKERDKTHAKQRLLLEYNGNKLWMNAIDGSEAILPTEFSKRYELSLFNTTNFGEDP
                     FPDVNLYNNMKSSFFVRFGGTSHPEAWAIYNASRKEVKYIETAREID"
     gene            complement(677..1441)
                     /locus_tag="ACHJ69_004448"
                     /pseudo
     CDS             complement(677..1441)
                     /locus_tag="ACHJ69_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(1717..3810)
                     /locus_tag="ACHJ69_004449"
     CDS             complement(1717..3810)
                     /locus_tag="ACHJ69_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HFO5017289.1"
                     /translation="MSSKISGLAFPCYAPPVFPEDGRLILSEAQVNANYLKQINKTEE
                     HKTNCSKQAGFRIGFTCNQSLHISLFFDGTNNNEYNDTPGHPTNIAKLFHTTYQNAEE
                     QGYFNYYIPGVGTPFPKIGEMDYSNSGLEFATGGEDRINWALLRLVDALSYSIDPSHK
                     RLDDNVAKEHIPLMRAPWPMTGEVNRRNVINPYLEKLQGLLEQASPRLLNVKLFIYGF
                     SRGAAEARTFVNWLTQLACPQEQQVMLAGLAVSIEFLGLLDTVASVGAAHVLPGAAGH
                     MGWADSTQQLPDERQFPGLIKCCRHFVAAHEQRLCFPLDSIRRPDGNYPLNAEEIIYP
                     GMHSDVGGGYPVRDQGKSCGDSGDILSQIALHDMYLAAFDSGAPLAVYSKFVTPLIKG
                     VSPLRIMSPSSVKEFTIANSLTKRFNIWRQTLLNTTLQGTEEMIDTREGYHPYQLAQV
                     LESAISDQIGWITAWRIGRYANNSLLVQPFYIKAPQLDKDGLDKEAQAREEQMENIEK
                     ERVNKQKGNGTLDESNQGIPILDPTNAQYQLREAAREFQSDYKGWTRDINGSVTERAI
                     QILVDVIPKYPVYLINGDDEAKEYQQIKAMGDGLYPQFFIDSLGTGTRTQPCAELLAL
                     YDDQVHDSRAWFMQSSLGGREPWGGYFRYRMIYFGDEANKELKLISADGEVVGDQPTS
                     NRVIYWMESKTVSRG"
     gene            complement(3795..4337)
                     /locus_tag="ACHJ69_004450"
     CDS             complement(3795..4337)
                     /locus_tag="ACHJ69_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFO5017290.1"
                     /translation="MSSKIRNGMNITKVVKGCLVLILMVISGCSAANYDDYTAGDLRA
                     INHIKGISINWFTVNGYRVHGLGGGYCCIMLPEKWHSGLKARIEWEVDPTPLEPIAMK
                     KEGFGYEEDAYAKHAANYQKYKVIVDIPEYGAERCGLTVHFLPCNQIKVTTVCEGYGT
                     PNYPIKEPREMKEPATCPAK"
     gene            complement(4322..4876)
                     /locus_tag="ACHJ69_004451"
     CDS             complement(4322..4876)
                     /locus_tag="ACHJ69_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFO5017291.1"
                     /translation="MSIKIRKSISMRAIFKCYSLMILFAVSGCSMARNSGDYTAVDIN
                     GINHTDKAINYIHINGYGGPNIGPFGDGGGYCCVMLPDKWHSGLKARVEWEVDPNTAP
                     LPPGYKDREKFEAWKKVALNSFRKYSTIVDIPEYDAELCGLTVHFLSCNQIKVTTVCQ
                     KYGTPNYPIKEPREMKEPATCPAK"
     gene            complement(4861..5414)
                     /locus_tag="ACHJ69_004452"
                     /pseudo
     CDS             complement(4861..5414)
                     /locus_tag="ACHJ69_004452"
                     /inference="COORDINATES: protein motif:HMM:NF023173.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
     gene            complement(5842..6618)
                     /locus_tag="ACHJ69_004453"
     CDS             complement(5842..6618)
                     /locus_tag="ACHJ69_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HFO5017292.1"
                     /translation="MITVSPHATDQDKYIIWLKQAESFCSAAGLDYIDVLVDQAGTDQ
                     PLQNEMLHLWPAIHWYALFEGTPEAETMEYSPVVLRLYFALSSHRKFLAQLMASFADT
                     PRLTLLISPLTFDLLGRHLQVLSQVQWEEQTGLLRYYDNRVFPLLFTHVLTNEQQATF
                     TDIALFWSWLDRDGEINWKSGSFSPERRLTDNPEMNRVDDVQVGLMGCICDAEALMKE
                     KAVSELSQESYFSHCLDIAIRANDAGIFRGFAGLRGIILW"
     gene            complement(6631..>7452)
                     /locus_tag="ACHJ69_004454"
     CDS             complement(6631..>7452)
                     /locus_tag="ACHJ69_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO5017293.1"
                     /translation="RSKTYKGSGFNELRFEDATDKEQVYIHAQKNMDTEVLNDRTTDV
                     KHDHTETIGNDQKITVVKGQTVQIGTRKEGGHDQSITVANDRCITVRNDQTLQVTNDR
                     TVSVSNDDGLYVRNDRKVTVEGKQEHKTTGNHVSLVEGKHSLVVKGDLARKVSGALGI
                     KVDGDIVLESSSRISLKVGGSFVVIHSGGVDIVGPKINLNSGGSPGAPVPTLQPAVLK
                     ALADDKSLAAEQNDSNASSDQNDNNDEGEEAPEKSVCKECLKRAQEAAAAFAPRG"
BASE COUNT         1934 a         1890 c         1452 g         2176 t
ORIGIN      
        1 tatcaatttc tcttgcagtt tctatatatt taacctcttt cctacttgca ttataaatag
       61 cccaagcctc gggatgggat gttccaccaa accttacaaa aaaacttgat ttcatattat
      121 tatataaatt aacatcagga aaaggatctt ctccaaaatt agtagtattg aataatgaaa
      181 gttcgtatcg cttagaaaat tctgttggta ggatagcttc actgccatca atagcattca
      241 tccagagttt gttaccatta tattccaaca ataatctctg ttttgcatgt gtcttatctc
      301 gttctttaac gacagcgaat acctttttaa caacttgctg tgcggatggt cctattgtat
      361 taccaaaagc tggcaacccg attttgccat aaacactctc ctgaatgaaa taaatatccg
      421 ttttatcggc ctcgacaata tctaccattt atccctccaa acaagacctg actccaggac
      481 aagagcatag tgtgtagtaa acacttcatg ctcttgccct gaccattgaa ataacaactt
      541 tcgtttgttt actgtacgct gtctaaatag ctgcgtggaa taaagttgca ctttttatca
      601 tcccttgcag agcaagaata cgaaagcccg cccccgcaag gactgacgcg aggtagtctc
      661 tgtccgcagc tgcttttcac atcttacatc ttgcccctgc cttgagtatc ttatctttcg
      721 ttaaaatatt aatagcgatc ttccgtatcc ctgaaaataa ttctgctgca tttcctcttc
      781 ttattttgaa gtcgtcttta ttcattacca cgtccagatg ccagtgcagc ttattctcca
      841 cgtaccagtg atttcggttc gctgtggcga acttctctgc ggttaaatca gcagaaatgc
      901 tataatatct gtccgtcatt tctggctctt tcttttgttc tgttattatt gaacgaaagg
      961 agactgccat gcataatttc ttcagctctt tccattcaaa cgtgaaatca ataagttcat
     1021 cagggacatc gcaaataata tgaagacgga ttttaatgac ataaatcgct cccttgcggc
     1081 gactcttgtc ataagagtgc cgaagcgttt ttccatcaat tgcaatgaca tctttatcat
     1141 ctgaggaatg gcagtcacgc atccagttaa taaagcactc atgaaatttt gcaggactga
     1201 tacaggatac aactctggca atgatatcgt gaacaggaat accattttca aaatcaccat
     1261 atcacttcaa aaaataggga tgtgtttccc caaatcctct atatcttccc agccttatgc
     1321 accaaaaata acggcgcaaa tagttaacag atagaatatc tggcaattta tgttccactt
     1381 tccaggcttg cctggaatcg gggataatag aaatatgttc catcaatttt ttaagatcca
     1441 ttttgccccc cttaattatg caatgagtat ttgatcatgc ataagcaatg aaaaacagct
     1501 ttaggtaata aggaacacct caattattga acataaaatg cgaattattt agtacaaaaa
     1561 gtgtactcct gatgatcttt ccctgattac ttgacctttc cctgggagta cagagtgaag
     1621 tttccggcat ggcgggatgc ggatgggttt gaactggggt gattaggcac tacatgtcta
     1681 cattttcccg gtctccaacc cctcaatctt atgaacttaa cctcggctca ccgttttcga
     1741 ttccatccaa taaatgaccc ggttggatgt gggctgatcg ccaacaactt cgccatctgc
     1801 agagattagc ttcagctctt tattagcctc atcaccgaag taaatcatgc ggtagcgaaa
     1861 ataaccgccc cagggttcgc gtccacccag cgaactttgc ataaaccagg cgcgggagtc
     1921 gtgaacctgg tcatcataga gagccagtaa ttcagcgcag ggctgagttc gcgttcccgt
     1981 tcccagtgaa tcaataaaaa attgtggata gagtccgtct cccatagcct taatttgctg
     2041 atattctttt gcttcatcat cgccattaat cagatacacc ggatatttag gaatgacatc
     2101 cacaagaatc tgaatggctc tctccgtgac gctgccgtta atatcgcgcg tccatccttt
     2161 ataatcgctc tgaaattctc gggctgcctc tcgcaactgg tactgagcgt tcgtcgggtc
     2221 aaggataggt atgccctgat tactttcatc aagtgtgcca ttgcctttct gcttgttcac
     2281 ccgctctttt tcgatatttt ccatttgttc ttcgcgcgct tgggcctctt tatcaagtcc
     2341 atctttatcc agttgtggcg ccttaatata aaaaggttgt acaagaagac tgttattcgc
     2401 atagcgaccg atacgccagg cggtgatcca gccgatctgg tcactaattg cgctttcaag
     2461 aacctgggca agttgatagg ggtgataacc ttccctcgta tcaatcattt cttctgtgcc
     2521 ctgcaaagtg gtgttcagca gcgtttgtcg ccagatgttg aagcgttttg ttaatgaatt
     2581 agcaatcgta aattccttta cgctagaagg actcataatt cgcagaggcg acacaccttt
     2641 tattaatgga gtaacaaatt tagaataaac agcaagtggt gctcctgaat caaatgcagc
     2701 aagatacata tcatgcaggg ctatctgaga aagaatgtcg cctgagtcac cacaggactt
     2761 cccctgatcg cgaacgggat agccaccacc gacatcagag tgcatccccg gatagataat
     2821 ttcctctgca tttaaaggat aatttccgtc tggacgacgg attgaatcca gcggaaagca
     2881 cagccgctgc tcatgcgcgg caacgaagtg gcggcagcat ttaatcaggc cgggaaactg
     2941 cctctcatca gggagttgct gagtactatc ggcccatccc atatgaccgg cagcgcccgg
     3001 taaaacatgc gccgccccca cagaagctac ggtatccagc agccccaaaa actcaatact
     3061 gacagctaac ccggccagca tcacctgttg ctcctgcggg caagctaact gagtcaacca
     3121 gttaacaaaa gttcgtgctt ccgctgcgcc acgggaaaat ccgtagataa acagctttac
     3181 gtttagcagg cgcggactgg cttgctctaa cagtccctgt aatttttcca ggtaggggtt
     3241 aatcacattg cgacgattca cttcaccagt cataggccag ggagcgcgca taagaggaat
     3301 gtgctctttt gccacattat catccagtcg cttatgggaa gggtcaatgg aatagctcag
     3361 ggcatccacg aggcgtaata atgcccaatt aattcgatct tcaccaccag tagcgaactc
     3421 cagcccgctg ttgctgtaat ccatttcacc tatcttggga aatggtgtgc ctacacccgg
     3481 tatatagtag ttaaaatatc cctgttcttc tgcgttctga taggtcgtat gaaacagttt
     3541 ggcaatattg gttgggtgtc caggagtgtc attatattca ttattatttg taccatcaaa
     3601 aaacagacta atatgcagac tttgattaca cgtaaagcca attctaaatc cagcctgttt
     3661 actacagttg gttttgtgct cttctgtttt attgatttgt tttaggtagt tcgcgtttac
     3721 ctgcgcttca gataatatta atcgcccatc ttcagggaac accggggggg cataacacgg
     3781 aaaagccaga ccacttattt tgctggacat gttgcaggtt ccttcatctc gcggggttct
     3841 ttgataggat aattcggtgt accatagccc tcgcagacgg ttgtcacttt aatctgatta
     3901 cacggcagaa aatgaacggt caggccacaa cgttcagcgc cgtattccgg gatatcaaca
     3961 attactttat atttttgata atttgcagca tgtttagcat aagcatcttc ttcatagcca
     4021 aaaccttctt ttttcattgc aataggttca aggggggtcg ggtctacttc ccactcgatc
     4081 cgggctttta atccagaatg ccacttctct ggtaacatta tgcagcaata tccaccgccc
     4141 aaaccatgga cccgataacc atttacggtg aaccaattaa tactgatacc tttaatatga
     4201 tttattgccc ggagatcacc tgcagtataa tcgtcgtaat tagctgctga gcaacccgaa
     4261 atcaccatca aaattaatac cagacagcct ttaactactt ttgttatatt cattccattt
     4321 cttattttgc tggacatgtt gctggttcct tcatctcgcg gggttctttg ataggataat
     4381 ttggtgtgcc atatttctgg cagacggttg tcacttttat ctgattgcac gatagaaaat
     4441 gaacggtcag gccacaaagc tcagcatcat attccgggat atcaacaatt gttgaatatt
     4501 ttctaaaact gtttagcgca actttcttcc atgcttcaaa tttctccctg tccttatagc
     4561 ctggaggcag aggagctgta tttggatcaa cctcccactc aactcgggct tttaatccag
     4621 aatgccactt atctggcagc ataacgcaac aatatcctcc accgtccccg aatggaccaa
     4681 tatttggtcc gccgtaacca ttgatgtgta tataatttat agccttatcc gtatgattta
     4741 tcccgttaat atccacagca gtgtaatcac cgctatttct cgccattgaa cagcctgata
     4801 ctgcaaataa aatcattaga gaataacatt tgaaaatcgc tctcatactt atacttttcc
     4861 ttattttgat ggacatgttg ctggttcctt catctcgcgg ggttctttga tgggataatt
     4921 tggtgtacca tacccttggc agactgttgt cacttttatc tgattgcatg gcagaaaatg
     4981 aacggtcatg ccacaacgct cagcaccgta ttccgggata tcaacaattg cggtatgttg
     5041 aatgtaactt tctttgagtt ttttctccca ggcctgatat ttatcccaat ccttatagag
     5101 tggtggcaga gggactgtat ttggtgtaat tccccactca atccgggctt ttaatccaga
     5161 atgccactta tctggcaaca taacgcaaca ataccccccc gctccccgaa cggaactata
     5221 ttttgcccgc catagccatt gatactgaaa taattaatag caacatcggt atggttcata
     5281 cctttaatat ctccagcagt gtaatcaccg ctatttctcg tcattgaaca gcctggcact
     5341 gcaaataaaa ctattagcga aaaatattta aaaatcgctc tcacaccaat accattcttt
     5401 atttcgacgg gcatattgct ggttccttta ctttgcgaga ttttgattaa ttggtattat
     5461 ttctacgaca acaaagcttt gacaaagaag caaataaaac acatatcttt cgattgtcac
     5521 acattgggat atcgacagag cattgatgat attacgcaaa atatattgca accacgtgaa
     5581 ctgacattac gatcgaacac aaatgcaaca tgatggatac taccattaca tgtcgagctt
     5641 gattttccgt ttatttcacg attatctctt gatattaaca gctacggaat tttaaaaaga
     5701 taaatataca catcgtcctt caagctgact ctgcgttggc tgcgttcact caccccagtc
     5761 acgtacttat gcacgctcct ggggattcgc ccgcttgccg ccttgatgca acttgaatga
     5821 ttttgtgtat acccctgtac atcaccacaa tattatccct cgaagtcccg caaagccccg
     5881 aaatatcccc gcgtcattgg ccctgatagc aatatcaaga cagtgcgaaa aatagctttc
     5941 ctgactgagt tcactgactg ctttctcctt catcagggct tcagcgtcgc agatacagcc
     6001 catcaatccc acctgtacat catccacccg attcatctcc gggttgtcag taagcctgcg
     6061 ttctggtgaa aaacttcctg atttccagtt aatctctcca tcacggtcta accagctcca
     6121 gaacaacgca atatcagtaa atgtcgcctg ctgctcgttg gtcaaaacat gtgtgaacag
     6181 taaagggaac acccgattat catagtaacg cagcaatccg gtctgttctt cccactgaac
     6241 ctgtgacaat acctgaagat gccgccccag caggtcgaat gtaagagggg atatcaacaa
     6301 agtaagacgc ggcgtatcag caaatgacgc catcagttgt gccagaaatt tgcgatgact
     6361 actgagcgca aagtacaggc gtaacactac aggggaatac tccatcgtct cagcttcagg
     6421 cgttccttca aacagggcat accagtgaat ggctggccac agatgaagca tctcattttg
     6481 caaaggttgg tctgttccag cctggtcaac aagcacatct atgtaatcca aaccagcggc
     6541 agaacagaat gattcagcct gcttcagcca gataatatat ttgtcttgat cggtagcgtg
     6601 tggcgatact gttatcattg tctttctccc ttatccacga ggcgcaaaag cggcggctgc
     6661 ttcctgcgcc cttttcaggc actccttaca caccgatttt tctggcgctt cctcaccctc
     6721 atcgttgtta tcattctgat cggacgatgc attactatca ttttgctcag ccgctaaact
     6781 tttatcatct gccagcgctt tcagaaccgc cggttgcagc gtcggcaccg gcgctcccgg
     6841 actaccgccg ctattgagat tgattttcgg cccgacgatg tccacgcccc ctgaatggat
     6901 gaccacaaac gagccaccca ccttcaggct tatccggctg ctgctctcca gcacgatatc
     6961 cccgtcgacc tttatcccca gcgcgccact caccttcctc gccaggtccc ccttcaccac
     7021 caggctgtgc tttccctcca ccaggctgac atggttcccc gtcgtcttat gctcctgctt
     7081 cccttccacc gtcactttac ggtcatttct gacgtacagg ccgtcatcat tactcacgct
     7141 caccgtccgg tcattcgtca cctgcagcgt ctggtcatta cgtaccgtga tgcaccggtc
     7201 gttcgccacc gtgatactct ggtcgtggcc cccttctttt ctggtcccga tctgaaccgt
     7261 ctgccccttc accaccgtta tcttctggtc gttcccgatg gtctccgtat ggtcatgttt
     7321 cacatccgtc gtccggtcgt taagtacctc cgtgtccatg ttcttctgcg cgtggatata
     7381 gacctgttcc ttatccgtcg catcctcaaa gcgcaactca ttaaaaccgc ttcctttata
     7441 ggtcttcgac cg
//
LOCUS       DAZFFE010000163         7383 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFFE010000163 DAZFFE010000000
VERSION     DAZFFE010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.164"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(35..1102)
                     /gene="purK"
                     /locus_tag="ACHJ69_004455"
     CDS             complement(35..1102)
                     /gene="purK"
                     /locus_tag="ACHJ69_004455"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO5017294.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPVTALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLSE
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQLQAQAEEMLSAIMQELS
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDLNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(1099..1608)
                     /gene="purE"
                     /locus_tag="ACHJ69_004456"
     CDS             complement(1099..1608)
                     /gene="purE"
                     /locus_tag="ACHJ69_004456"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO5017295.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNEWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(1704..2033)
                     /locus_tag="ACHJ69_004457"
     CDS             complement(1704..2033)
                     /locus_tag="ACHJ69_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017296.1"
                     /translation="MQRCNGFLISGIALTAGAPLCISWFGLVGFYFGALSLVAAAVIS
                     WIVYFWLIYLVLTRPHNQRFVERFILIVGICFFPWSIVLIWAFFASSARAHNQHAEQI
                     KNSTTDG"
     gene            complement(2144..2866)
                     /gene="lpxH"
                     /locus_tag="ACHJ69_004458"
     CDS             complement(2144..2866)
                     /gene="lpxH"
                     /locus_tag="ACHJ69_004458"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO5017297.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHHQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQTAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(2869..3363)
                     /gene="ppiB"
                     /locus_tag="ACHJ69_004459"
     CDS             complement(2869..3363)
                     /gene="ppiB"
                     /locus_tag="ACHJ69_004459"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO5017298.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKDPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            3537..4922
                     /gene="cysS"
                     /locus_tag="ACHJ69_004460"
     CDS             3537..4922
                     /gene="cysS"
                     /locus_tag="ACHJ69_004460"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO5017299.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(4970..5479)
                     /locus_tag="ACHJ69_004461"
     CDS             complement(4970..5479)
                     /locus_tag="ACHJ69_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5017300.1"
                     /translation="MPTVITHAAVPLSIGLGLGSKAIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMLLMGMLW"
     gene            complement(5587..5799)
                     /gene="ybcJ"
                     /locus_tag="ACHJ69_004462"
     CDS             complement(5587..5799)
                     /gene="ybcJ"
                     /locus_tag="ACHJ69_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO5017301.1"
                     /translation="MATFSLGKHPHVEQCDLLKLEGWSESGAQAKIAIAEGLVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(5801..6667)
                     /gene="folD"
                     /locus_tag="ACHJ69_004463"
     CDS             complement(5801..6667)
                     /gene="folD"
                     /locus_tag="ACHJ69_004463"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO5017302.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            6708..6839
                     /locus_tag="ACHJ69_004464"
     CDS             6708..6839
                     /locus_tag="ACHJ69_004464"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017303.1"
                     /translation="MSILSEAWRAVSFRFLKSKTFCTWLIHTYHDNRRWFAAIVSYI"
     gene            6938..7014
                     /locus_tag="ACHJ69_004465"
     tRNA            6938..7014
                     /locus_tag="ACHJ69_004465"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6972..6974,aa:Arg,seq:tct)
     gene            complement(7030..7383)
                     /locus_tag="ACHJ69_004466"
                     /pseudo
     CDS             complement(7030..7383)
                     /locus_tag="ACHJ69_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232088.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1785 a         1976 c         1921 g         1701 t
ORIGIN      
        1 tttacgccgc atccggcaag aaaatgagca tcaattaact gaacttgctc tgcgcccaca
       61 tcacgccgct ggcatactcc ggcggcagca gtgggattaa ggcttccagc gtcgcggtca
      121 gacgcgatgt gtcgctgtcg gtcaaattca aatgccccac tttacggccc ggacggactt
      181 ctttgtcgta ccagtgcaga tgcacaagcg gcagtttcag ccagtcataa ttcagatcgc
      241 taccaatcag attgatcatc accgacggat tattcactac tggttgcggt aacggcagat
      301 cggtaatcgc ccgcagatgt agctcaaact ggctgatgct ggcaccgttt tgcgtccagt
      361 gaccgctgtt atgcacacgc ggagccagtt cgttgatcag cagaccttgt ggggtgacaa
      421 aacactccat tgccatcacg cctacatagc tcagctcctg cataatcgcc gacaacatct
      481 cttcagcttg cgcctgcagc tgtgcgttgg cctgcgggaa agcgacgctg gtgcgcaaaa
      541 tgccgtcctg atgcaggtta tgcgtcagcg gataaaacac ggtgctgcca tcaaaaccgc
      601 gcgcgccaac cagcgacact tcaccagaga agtttatgcc ctgctcgaca atacattcgc
      661 cgtagcattc agcaggtaac tgttcggttt catcggcgcg taaacgccat tgaccgcgtc
      721 cgtcatagcc accagtgcgg cgcttaacaa tcgccagctc acctaaacga tcaaacaccg
      781 caggccactc gctgcgttcg gaaagtaact gccacggtgc ggtcggcagg tggagcttat
      841 cgaaaagctg cttctgagtc agacggtcgg caataatcgg gaacacatcg cggttcacaa
      901 aggccggatg gcgcgccagc tcgcgggtta atgcagttac cggccagcgt tcgatctcag
      961 cggtaatcac gctttgttga aaaggcaccg ccgccggttc agcgtccagc ccgaccggcc
     1021 agacagcaat gcctaacggt tcgcctgcct gacgcagcat acggcctaac tgcccgttac
     1081 cgaggacgca aacctgtttc atgccgcacc tcgcgggtcc gggttttcca gcacttcatc
     1141 agtttgtgct ttgcgccatt cattcagacg ctggtgcagt tctttatcat gagtcgcaag
     1201 aatttgtgct gccagtaacg ccgcgtttgc cgcgccagct ttaccaatcg ccagcgtacc
     1261 caccggaatg ccgcgcggca tttgtacgat ggagtagagg ctatcgacac cgctcagtgc
     1321 ggcgctctgt actggcacgc ccagcaccgg caccagcgtt ttggcggcaa tcatgccagg
     1381 caggtgcgct gcgccgcctg cgcccgcaat aatcacctga taaccgttct cttcggcgct
     1441 ttcggcgaag ctgaacagtt tatcgggggt gcggtgagca gaaaccactt caacgtggtg
     1501 cgggacattc aggatttcga agatttcggc ggcgaactgc atggtagccc agtcgctttt
     1561 ggaccccatc acgatggcga cacgcgccgg attattgcgg gaagacatgc gtcttaaaac
     1621 tcctgtggtg cacaactctc ggctttagag ggcacagaga atagcacgga aagagtgcaa
     1681 ggaaaacggt tgcgtaggcg gaattaaccg tcagttgttg agtttttaat ttgttctgcg
     1741 tgttgattat gcgctctggc tgaactggca aagaatgccc atatcaacac aatgctccat
     1801 ggaaaaaagc aaataccgac aatcaatata aatctttcta caaaacgttg gttatggggg
     1861 cgggtgagta ctaaataaat gagccagaaa tagactatcc aggaaatgac cgccgcagct
     1921 accagtgaca atgcgccaaa atagaagccg acaagaccga accagctaat acacagtggc
     1981 gctcctgccg taagggcgat gccagaaatc aaaaatccgt tgcagcgttg cataataata
     2041 ttccctgttg ttatttcact cagagattat taccttgctg attttaaaaa agatatacag
     2101 gttgcgttag ttcggtgctt tttcagaatc tgtccattcg tggtcagaaa ggaaaatgga
     2161 ttagctcaac gtcatccgcc gtgactttca ccatcgaacc ttctgtatgc caggcaccca
     2221 gtaccacgcg aaaagccgtt tgctgattgg cgataagttc atgcaccgcc gggcgatggg
     2281 tatgcccgtg gatcagccat tgcacttgat gtttttccat cgcactgacc accgcgtttt
     2341 ggttaacgtc catgatcgcc agcgatttac tgctgttggc ttctttactg ttcgcgcgca
     2401 ttcgcgcggc aatgcgtttg cgcacaaaca acggcagggc gaggaatagc gtttgcagcc
     2461 agggtttgtg gactttggca cgaaaagcct gataacccgc gtcatcggtg cacagcgtgt
     2521 cgccatgcat aatcaacacc cgacgaccat aaagttcgag caccttttct tccggcaata
     2581 acgtcatgcc gctttcacgg gcaaagcgtt tgccgagcag aaaatcacgg ttgccatgaa
     2641 tgaaataaca gggaacgccg gaatcggata ccgctttgat cgctgcggcc atctggtgat
     2701 gaagtgggtt gggatcgtcg tcgccgatcc aggcttcaaa cagatcgcca agaatataca
     2761 gcgcatcggc cttgcgggcc tcccccgcta aaaaacgcag aaaaccggcg gtgatcgccg
     2821 gttcttccac gcagagatga agatctgcaa taaagagtgt cgccacgatt actcgctaac
     2881 ggtcacgctt tcaatgataa cgtcttcttt tggcacgtcc tggtgcatac cgctacgacc
     2941 ggttgccaca cctttgattt tgtccaccac gtccatgcct tcaaccactt cagcaaatac
     3001 gcagtagccc caaccttgca ggctttcgcc agagaagttc aggaagtcgt tatcaaccac
     3061 gttgatgaag aactgggcag ttgcagagtg cggtgcctga gtacgggcca ttgccagcgt
     3121 accacgggta tttttcagac cgttgttcgc ttcgtttttg atcgggtctt tggtggcttt
     3181 ttgtttcatg cccggttcaa aaccgccgcc ctgaatcata aagccgttga taacacggtg
     3241 gaaaatggtg ttgttgtaaa aaccttcgcg gcagtagtcc aggaagtttt taactgtttc
     3301 aggtgcttta tcgtcaaaag ttttgatgac aatatcgccg tgattggtgt ggaaagtaac
     3361 catttttgca tcctgttccg tttgattggt gcttcaaccc ggttcgggtc atatataggg
     3421 tggtgttata gcataaccgc acgatcggat catctcgcaa tgtatgctga ttcgcgcggg
     3481 aaatatgggt attatacgca actcaattac ccacacatgt ctaaacggaa tcttcgatgc
     3541 taaaaatctt caatactctg acacgccaaa aagaggaatt taagcctatt cacgccgggg
     3601 aagtcggcat gtacgtgtgt ggaatcaccg tttacgatct ctgtcatatc ggtcacgggc
     3661 gtacctttgt tgcctttgac gtggttgcgc gctatctgcg tttcctcggc tataagctga
     3721 agtatgtgcg caacattact gatatcgacg acaaaatcat caaacgcgcc aatgaaaatg
     3781 gcgaaagctt tgtggcgctg gtggatcgca tgatcgccga aatgcacaaa gattttgatg
     3841 ctttgaacat tctgcgcccg gatatggagc cgcgcgcgac gcaccatatc gccgaaatta
     3901 ttgaactcac tgaacaactg atcgccaaag gtcacgctta tgtggcggac aacggcgacg
     3961 tgatgttcga cgtcccgacc gatccgacat atggcgtgct gtcgcgtcag gatctcgatc
     4021 agctgcaggc aggcgcgcgc gttgacgtgg tcgacgacaa acgcaacccg atggacttcg
     4081 ttctgtggaa gatgtcgaaa gagggcgaac cgagctggcc gtctccgtgg ggcgcgggcc
     4141 gtcctggctg gcacattgag tgttcggcaa tgaactgcaa acagttgggt aaccactttg
     4201 atatccacgg cggcggttct gacctgatgt tcccgcacca cgagaacgaa attgcccagt
     4261 ccacctgtgc gcacgatggc cagtatgtga attactggat gcactccggt atggtgatgg
     4321 ttgaccgcga gaagatgtct aaatcgctgg gtaacttctt caccgtgcgt gacgtgctga
     4381 aatactacga tgcggaaact gtgcgttact tcctgatgtc gggtcactat cgcagtcagt
     4441 tgaactacag tgaagagaac ctgaaacagg cgcgtgcggc gctggaacgt ctctatactg
     4501 ccttgcgcgg cacagacaaa accgttgcgc ctgccggtgg cgaagcgttt gaagcgcgct
     4561 ttattgaagc aatggatgac gatttcaaca ccccggaagc ctattccgtg ctgtttgata
     4621 tggcgcgtga agtgaaccgt ctgaaagcag aagatatggc agcggcgaat gcaatggcgt
     4681 ctcacctgcg taaactttct gccgtattgg gcctgctgga gcaagaaccg gaagcgttcc
     4741 tgcaaagcgg tgcgcaggca gacgacagcg aagtggctga gattgaagcg ttaattcaac
     4801 agcgtctgga tgcccgtaaa gcgaaagact gggcggcggc agatgcggcg cgtgaccgtc
     4861 ttaacgagat ggggatcgtg ctggaagatg gcccgcaagg gaccacctgg cgtcgtaagt
     4921 aattgcgcta ttgccggatg cgagttttcg catccggtta tcgtctgcgc taccacaaca
     4981 ttcccatcag taacatcccc ggcaaccaca cccacatcaa ttcagaaata atcacctgat
     5041 gcccgtacgg cgtggtgtaa cgagacaatg caaacggcgc gactttaatc acctgccagg
     5101 gtgcgaaaaa gcgttcatct gaccacggcc acagccagcc aaccccttta ccgccagtgg
     5161 ttaccgaatc cagcaagctg tgcgatagca acgagacggt taaaaacagc cagcagcgaa
     5221 tcagcccagc cctgaaccat cgacgtccaa tcagcacaca taacagcggg acaacaaacg
     5281 caaacaccag cgaatgggta aacccgcgat gaccaaaaac atttccgtaa gcaacgccaa
     5341 atttaaacga caatacatcg gcatcgggca gcatcgccag gatgattccg gcaaataaca
     5401 gacgcggagg gatggctttc gaacccaacc ctaaaccaat gcttagggga acggcggcgt
     5461 gcgtaataac ggttggcatg atggtcgctt cggcaaaatg tcgatgctat cagcatggat
     5521 gaacggggcg tagagggcaa aagtctgaaa agagaaccgg cctgttgata caggccggga
     5581 aagggatcag gcaacaacct gtacgctgtg acctgcaaaa ctcactgtct gaccggcgac
     5641 gattttgcag cgtttgcgcg tttcaaccgc accgtcgact ttcaccaggc cttcggctat
     5701 cgcgattttc gcctgcgcgc cgctttcgct ccagccttcc agtttcagca agtcgcactg
     5761 ctcaacgtgc ggatgtttac ctaaagaaaa tgtcgccatg ttactcaccc tgtggatcat
     5821 gatattcaac gcatgcctgt agcgtgtttt caatcagcgt ggcaaccgtc atcgggccaa
     5881 cgccgcccgg aacaggcgta atgtatgagg cgcgtttagc cgcgtcttca aacacgacgt
     5941 cgcccacaac tttgccattt tccagacggt tgatgccgac atcaatcaca attgcgcctt
     6001 ctttgatcca gtcaccagga ataaagcccg gcttgccgac ggcaacgatc aacaaatcgg
     6061 cattttctac gtggtgacgc agatttttag tgaaacggtg agtcacggtg gtggtgcaac
     6121 ctgccagcag cagttccatg ctcattgggc gaccgacgat attcgatgcg ccaatcacca
     6181 cggcgttaag gccgaaggta tcaatgttgt aacgctcaag cagcgtgacg ataccgcgcg
     6241 gggtacaggg acgcagacgt ggcgcgcgct ggcacagacg accgacgttg taaggatgga
     6301 aaccgtccac gtctttgtcc ggatgaatac gttccagcac tttgacgtta tcaatacccg
     6361 ccggtaacgg cagttgaacc agaatgccat caatggtgtt gtcggcattc agcgcatcga
     6421 taagttccag cagctccgct tcgctggtgg tttccgggag gtcataagag cgggagacga
     6481 acccgacttc ttcacaagcc ttgcgtttgc ttgcgacata aatttgcgat gcagggttgc
     6541 tacccaccag cacaacggcc agtcctggcg cccgcagtcc ggctgcaata cgcgcctgaa
     6601 ctttttgagc aacttcagag cgcacctgct gcgcaatcgt tttaccgtca ataatctttg
     6661 ctgccatcag agagaggatt ccatctgtta cgtaggtcga aggggatgtg tctattttgt
     6721 cagaagcgtg gcgcgctgtc agttttcgtt ttctgaaaag taaaaccttc tgtacctggc
     6781 tgattcacac ctatcatgat aaccgtcgtt ggtttgccgc catagtgagc tacatctgat
     6841 gcggtcgtac acttgttcag caactggttc aaaagccatt gactcagcaa gggttgaccg
     6901 tataattcac gcgattacag cacaccgtgg tatcaatgcg cccttagctc agttggatag
     6961 agcaacgacc ttctaagtcg tgggccgcag gttcgaatcc tgcagggcgc gccattaaaa
     7021 ttcaatcagt tacgccttct ttatttcctc tataattcca gagtgggaca ttatcaccaa
     7081 aaatgtcgtc tattttcctc gcgtgctcag tcaaatgatt aggcgcaagg tgagcatatc
     7141 tacgaaccat ttctctggac tcccatccgc ccatttcctg aagcactgat aatgggacgc
     7201 ctgacggaat caacctgctt gcccgggtat gccagaggtc atggaaacgg aaattttcaa
     7261 ctcctgcacg acgacaagct gatagccatg ctgtcttgct gtcaatgcgc atcttcctga
     7321 gcgcaggcat tgatgttcca tctgctcgct tagccgccct ggtatgtaca cccacatttg
     7381 tga
//
LOCUS       DAZFFE010000164         7372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFFE010000164 DAZFFE010000000
VERSION     DAZFFE010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.165"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            71..607
                     /locus_tag="ACHJ69_004467"
                     /pseudo
     CDS             71..607
                     /locus_tag="ACHJ69_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            565..1242
                     /gene="bglJ"
                     /locus_tag="ACHJ69_004468"
     CDS             565..1242
                     /gene="bglJ"
                     /locus_tag="ACHJ69_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO5017304.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSARREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(1280..2068)
                     /gene="fhuF"
                     /locus_tag="ACHJ69_004469"
     CDS             complement(1280..2068)
                     /gene="fhuF"
                     /locus_tag="ACHJ69_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO5017305.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPLMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHVHVEFHETGRAACFWVDVCEDKNATLHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRYALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2209..2445
                     /gene="yjjZ"
                     /locus_tag="ACHJ69_004470"
     CDS             2209..2445
                     /gene="yjjZ"
                     /locus_tag="ACHJ69_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO5017306.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2484..2570)
                     /locus_tag="ACHJ69_004471"
     tRNA            complement(2484..2570)
                     /locus_tag="ACHJ69_004471"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2534..2536),aa:Leu,seq:cag)
     gene            complement(2605..2691)
                     /locus_tag="ACHJ69_004472"
     tRNA            complement(2605..2691)
                     /locus_tag="ACHJ69_004472"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2655..2657),aa:Leu,seq:cag)
     gene            complement(2720..2806)
                     /locus_tag="ACHJ69_004473"
     tRNA            complement(2720..2806)
                     /locus_tag="ACHJ69_004473"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2770..2772),aa:Leu,seq:cag)
     gene            complement(2997..4028)
                     /gene="rsmC"
                     /locus_tag="ACHJ69_004474"
     CDS             complement(2997..4028)
                     /gene="rsmC"
                     /locus_tag="ACHJ69_004474"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO5017307.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDANKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLATNGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4131..4544
                     /gene="holD"
                     /locus_tag="ACHJ69_004475"
     CDS             4131..4544
                     /gene="holD"
                     /locus_tag="ACHJ69_004475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO5017308.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPPHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4513..4959
                     /gene="rimI"
                     /locus_tag="ACHJ69_004476"
     CDS             4513..4959
                     /gene="rimI"
                     /locus_tag="ACHJ69_004476"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO5017309.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            4974..5651
                     /gene="yjjG"
                     /locus_tag="ACHJ69_004477"
     CDS             4974..5651
                     /gene="yjjG"
                     /locus_tag="ACHJ69_004477"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO5017310.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            6037..7254
                     /locus_tag="ACHJ69_004478"
     CDS             6037..7254
                     /locus_tag="ACHJ69_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029397014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5017311.1"
                     /translation="MALSDAWLRSVVGKERDKVLVKSDRDGLSVRVSPKGRVVFQYRY
                     QWAGKGERLDIGTYPATGLKEAREEVIRLRGELESNRNPRLVKQAEKRKATEAMTVES
                     VIRAWYEAYYVKNKKGSEQILRSFELHLFSKIGNIPHDAATLHDWLEVLEPLSTKTPA
                     IADRLLINAKQAHVWAYKRKLIETRPLSDITGKDMDIRKGQKKRFLTHDEIKILYAAI
                     DGSRMVPKYRAFIKLLLHFGCRSSELITARVDDFDFINKVWTVPPERHKTGDITGEPL
                     KRPIIEPVEELIKYVISMNNGSDMLFTKEGSREPVGRTSLQSLPYNLMQYAWRRLGYQ
                     FPHWSLHDLRRTARTNFSDLTAPHIAEIMLGHKLPGVWQVYDKSDYLEEQRKAYQAWW
                     ERVESIVTCTCSD"
BASE COUNT         1888 a         1754 c         1868 g         1862 t
ORIGIN      
        1 gagctgcatg acaaagtcat cgggcattat ctgaacataa aacactatca ataagttgga
       61 gtcattacct gaagatccac gttccgtttg tgagcattat tactctttaa tctcacaata
      121 tcgggaaatt cactgggttt tcatggtgtc acgctcctgg tattcccagg cagtagaatt
      181 gctcatgtgc cctacggcga cgttattgtc tgatgttgaa cccattgaga atctggtcaa
      241 gaccgtacgt tccggcaata cgcactcaga gcgtatcagc gccatgctga cctctccggc
      301 gatgactgaa actcatgatt ttagctatcg ctccgtcatt ctcactcttt cagagcgcaa
      361 ggtactgcgg ctattaggta aaggatgggg catcaaccag atagcttcat tgcttaagaa
      421 aagtaataaa actattagcg cccaaaaaaa cagtgcgatg cgtcgactgg caattcacag
      481 caacgctgaa atgtatgcat ggataaatag cgcgcagggt gcaagagaac ttaacttgcc
      541 ttctgtttat ggagatgccg cagaatggaa cacagccgaa ttaagaagag aaatgtcgca
      601 ctcatagaaa aatgcgtcat gagtagtatc ggtattgaga gtttattcag aaagtttgca
      661 ggtaaccctt ataagctcca tacctatacc agtcaggagt catttcagga tgccatgtcg
      721 cggatctcgt ttgcagcggt cattttttct ttttctgcca tgagaagtgc gcgcagagag
      781 ggattatcct gcctgactga actggcgatt aagtttccgc gtacccggcg tttagttatt
      841 gcggatgatg atattgaagc tcggctgatt ggttcattgt cgccatcacc gctggacggt
      901 gtattaagta aagcgtcaac gctggagatt tttcatcagg aactcttttt gtcattaaat
      961 ggtgtacgtc aggcgaccga tcgactgaac aatcagtggt acattaacca aagccggacg
     1021 ttaagcccga cggagagaga aatattgcgc tttatgtcgc gtggctactc aatgacacaa
     1081 attgccgagc agcttaaacg caatattaaa acgatccgtg cacataaatt taatgtgatg
     1141 tcgaaactgg gcgtcagttc tgatgcaggg ttgttggagg ccgcagatat tctgttatgt
     1201 atgcggcatt gcgaaacaag taatgtgttg catccttatt aatccgcatg atgccgggtt
     1261 tacttccccg gcagtgcttt catttcagcg tacaatcgcc acattgctgc acatccggta
     1321 agcgataacg ctggcagcaa gtgcggcgca ccagcaggcc gtcgcgtagc accacggtac
     1381 gccagagtgg attatcttca ccggtagtaa gcgttttctc aaaaaagagg gcatagcgca
     1441 gcgattcaac cgtcgcctcg ccgagcagct gtttcatctc agtcagatac cagttgatca
     1501 aataaccggt attgctccag ataagtttgc cgttaatctc tcccgttgct tctagtgcct
     1561 gcacaaccgg aaccagcgcc tggctgatta acgtttccat tcgctgctgc ggcgaatgta
     1621 gtgttgcgtt tttatcttca cacacatcga cccagaaaca ggcggcgcgt ccggtttcgt
     1681 gaaactcaac gtggacatgt tccggcgaca catctaatgc tttttcctga gtcagcagcg
     1741 ccagcattaa tggtggtacc atcaggccga tataccattg cgcccacagt gagatcagcg
     1801 gtttgttttc gcggatcatc aaaggttggt tgcggtagat atgatcggaa tagaccgcga
     1861 gtagagaact tagcgcatta ggtgatgacc attgcgccag cgtcatggcg ttaagtgggg
     1921 cgggttcatc caggcggata aactccagca aatgttcacg atgttcggcg atcgtcgccc
     1981 gcacggcttg tgcaagccca gcatcctgcg gctggagatg cgttcgccag ataacatctt
     2041 catagagcgg tgcggaacga taggccataa tcgggatagt aatctaaatg ataatgattg
     2101 ctaatcctat cgataggttt acccgatagc aagggatttc tctggcttgc aaatgataaa
     2161 aattatcata tgatattggt tgtcattatc aatgaaagag atgaaaccat gttgcaacgt
     2221 acgctgggca gtggctgggg agtgttgctg ccgggattgc tgattgcagg gctgatgtat
     2281 gcggatttat cgccggatca atggcggatt gtcattctga tgggattggt attgacgccg
     2341 gtgatgctgt atcacaaaca gttacggcat tacgttttgc taccatcgtg cctggcgctt
     2401 attgctggca tcatgctgat gataatgaat ttgaatcagg ggtgataaaa cagtgaagag
     2461 acaagaaaag aagtaaagat aattggtgcg agggggggga cttgaacccc cacgtccata
     2521 aggacactaa cacctgaagc tagcgcgtct accaattccg ccaccttcgc acagtcatct
     2581 tacttttttt gataccgcct cgtttggtgc gagggggggg acttgaaccc ccacgtccgt
     2641 aagaacacta acacctgaag ctagcgcgtc taccaattcc gccaccttcg cccagtgcga
     2701 gcaatatcaa cgtggttttt ggtgcgaggg gggggacttg aacccccacg tccgtaagga
     2761 cactaacacc tgaagctagc gcgtctacca attccgccac cttcgcatac catcaattct
     2821 taaaaagaat tgctaccacg gaggcgcatt ctagtggttt tcagcttttc gtcaatagtt
     2881 aattatcgac agaggtgtaa ttgctggaaa aatgtccatc agcggattgt ggataattcg
     2941 tttgtcggtc agataaggcg tgtacgccgc atccgacaag gcatgcggcg agatacttaa
     3001 cctttcttcg cctggcgggt cataatggcg cgatacacct tgaagcgccc ggtttgcgca
     3061 atcacttcgt ggaaaccaaa tgtctcatcc agcacgtccg ggtaaggcag gaaggcgttc
     3121 gctacaattc gcaactcgcc gccgctgtta agatgacgca ccgcgccgcg aattagcgtt
     3181 tgcgccgcat ccaggctggt ttgcatccca tcgtggaacg gcgggttgga gattatcata
     3241 tcaaagcgac ctttcacctc ggaaaagacg ttgctggcaa agacttcacc ttcaacaccg
     3301 ttggtcgcaa gtgttgcgcg actggcttct accgctggtg cagaaacatc acacagagtg
     3361 aggcggatct tcggtgaatg gcgcgcaaaa gcaacggaaa gaacgcctgc gccgcagccg
     3421 acatccagca ctttaccttt cgtgtgtgga gttagcgtcg agagcagcaa ctgactgccg
     3481 acatccagac cgtcgcggct aaacacgccc ggcagcgttt tgaccgtcaa gccatcgacg
     3541 ctgtattcgc cccagaattt attggcatca aataccggct gcttttccag acgaccaaaa
     3601 tagaggccgc agcgacgggc gctgtctacc ttattcaacg gtgcataatc ggccagcatc
     3661 tgctcggcgc tgcgcacgcc gctgcggttc tcgccaacga caaaaatatc cgtgcccacc
     3721 ggaagcagag aaagtaaatt catcaactgg aactgggctt ccggtttgtt cttcggccag
     3781 tagtagatca gcgtatcgca atctgcgacg tcatctgccg tggcgaccag gctaaaacgg
     3841 gcgttatccc ccatctggcg gcttaacacc tgccagtggt ggaattgctg ggtatgagca
     3901 cggctggccg cggtatctaa acgcgcgggc aggtcatcct gtaagtctcc ggcaaacaga
     3961 atacggcttt gttcgaaatc atcactgtga cgcagcaaga cttcacttgc cggggtaaat
     4021 gcagacatgg aatgctcctc aattgatact ggcggcgatt atagccatat gttggcgcgg
     4081 tatcgacgaa tttgctatat ttgcgcccct gacaacagga gcgattcgct atgacatccc
     4141 gacgagactg gcagttacag caactgggca ttacccagtg gtcgctgcgt cgccctggcg
     4201 cgttgcaggg ggagattgcc attgcgatcc cgccacacgt ccgtctggtg atggtggcaa
     4261 acgatcttcc cgccctgacc gatcctctgg tgagcgatgt tctgcgtgcg ttaactgtca
     4321 gccccgatca agtgctgcaa ctgacgccag aaaaaatcgc gatgctgccg caaggcagtc
     4381 gctgcaacag ctggcggttg ggtactgacg aaccgctatc actggaaggc gctcaggtgg
     4441 catcaccggc gctcaccgaa ttacgggcaa acccaacggc acgcgccgcg ttatggcaac
     4501 aaatttgcac atatgaacac gatttcttcc ctcgaaacga ctgatttacc agcggcttac
     4561 cacattgaac aacgcgccca cgcctttccg tggagtgaaa aaacctttgc cagcaaccag
     4621 ggcgagcgtt atctcaactt tcagttaacg caaaacggca aaatggcggc gtttgcgatt
     4681 acgcaagtgg tgctggatga agctacattg ttcaatattg cggtcgatcc tgactatcag
     4741 cgtcagggat tgggaagggc gctgctggaa catctgatcg acgaactgga aaaacgcggc
     4801 gtggcgacgc tatggctgga agtccgtgct tcaaacgctg ccgccattgc cctgtacgaa
     4861 agtttaggct ttaacgaggc gacaattcgc cgcaattact accccaccac ggacggtcgc
     4921 gaagacgcca tcatcatggc gttgccaatc agtatgtaag acaaggtgga ataatgaagt
     4981 gggactggat tttctttgat gccgatgaaa cgctgtttac ctttgactca ttcaccggcc
     5041 tgcagcggat gtttcttgat tacagcgtca cctttaccgc tgaagatttt caggactatc
     5101 aggccgttaa caagccactg tgggtggatt atcaaaacgg cgcgatcact tcattacagc
     5161 ttcagcacgg gcggtttgag agctgggccg aacggctgaa cgtcgaaccg ggtaaactca
     5221 acgaggcctt tattaatgcg atggcggaaa tctgtacgcc gttgccgggg gcggtttccc
     5281 tgcttaacgc cattcgtggc aacgccaaaa tcggcatcat caccaacggc tttagcgcct
     5341 tgcaacaggt acgtctggaa cgcacgggcc tgcgtgatta cttcgatttg ctggtgattt
     5401 ccgaagaagt tggcgttgcc aaaccgaata agaaaatttt cgattatgcg ctggaacaag
     5461 cgggcaatcc tgaccgttca cgcgtgctga tggttggtga cactgccgag tccgatattc
     5521 tcggtggcat caacgccggg cttgcgacct gctggctgaa tgcgcacaat cgcgagcaac
     5581 cagaaggcat cgcgcccacc tggaccgttt cttcgttgca cgaactggag cagctcctgt
     5641 gtaaacactg attgcctccc ccccgttgat gggtaaaata gccgcaattt ttcgttttca
     5701 acaagcgcgg cgcgatgccg cttactcaag aagaaagaat tatgacgttg tctccttatt
     5761 tgcaagaggt ggcgaagcgc cgcacttttg ccattatttc tcaccccaat aattaagccc
     5821 aaattaaagc tcttttactc tttcaaaatc ctttcagtta attgagtaat aattacacaa
     5881 ctcatttaat aacatcatgt tatgtggttt ctttaattga aagttctttc atgtaacttt
     5941 aaatcgatca ggttactttc atcaaaaatc tgtacatgtg cttgtacata atgtacaaaa
     6001 cagcagaggt gttttgttat ttgtacaagg tgagtaatgg cgctgtctga tgcgtggttg
     6061 cgttcagtcg ttggaaagga acgtgataag gttttggtta aatccgatcg tgatggtctg
     6121 tctgtcagag tatcaccgaa aggtcgcgta gtgttccaat atcgttatca atgggcaggg
     6181 aaaggtgagc gtcttgatat cggaacttac ccggcaactg gattaaaaga ggccagagaa
     6241 gaagttatcc gtctccgtgg tgaactcgag tcaaaccgta atccacgatt ggtcaagcag
     6301 gctgaaaaac gaaaagctac tgaagccatg acggtagagt ctgtgatccg tgcctggtat
     6361 gaagcatatt atgtaaaaaa taaaaagggt tctgaacaga tactccgctc gtttgagctg
     6421 cacctgttct ctaaaatcgg gaatatccct cacgatgcag ctacattgca tgattggtta
     6481 gaagtcttgg agcctcttag cactaagact ccagcaatag cagaccgatt gctaattaac
     6541 gcaaagcagg cccatgtctg ggcgtataag agaaagctta ttgaaactcg cccgctgtcg
     6601 gatatcacgg gtaaagatat ggatatccgt aaaggtcaga agaaacggtt tctgacacac
     6661 gatgaaatta aaattcttta tgctgcgatc gatggctcta gaatggttcc caaataccgg
     6721 gccttcatta aactattact gcattttggt tgccgtagtt cggagctaat tactgctagg
     6781 gtggatgatt ttgatttcat aaataaggta tggactgtac cgccagaacg acataagaca
     6841 ggggatataa caggcgaacc gctaaagcgc cccattattg aaccggttga agagcttata
     6901 aagtacgtta tctctatgaa caatggttcc gatatgcttt ttactaagga aggaagcagg
     6961 gagcccgttg gacggacatc attgcagtcg ctgccttaca atttaatgca gtacgcatgg
     7021 cggcgtttgg ggtatcaatt ccctcattgg tctcttcatg atttgagacg aacagcacga
     7081 acaaactttt ctgatcttac tgcgcctcat attgcagaaa taatgctcgg tcataaactg
     7141 ccaggggtat ggcaggttta tgacaagagc gattatttag aagaacagcg taaagcttac
     7201 caggcgtggt gggagagggt tgaatcgata gttacttgta cttgttcaga ctagaactga
     7261 cattttgcat aggcagaacg caattatgta tattgcggta taatcccctt cttaaaactg
     7321 taagaattgc ttgctaatcc ttgcagtttt tgattataac atttttattt gt
//
LOCUS       DAZFFE010000165         7368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZFFE010000165 DAZFFE010000000
VERSION     DAZFFE010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.166"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..87
                     /locus_tag="ACHJ69_004479"
     CDS             <1..87
                     /locus_tag="ACHJ69_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017312.1"
                     /translation="TLYWIENGIFQTSVPYVVPYLDKFLASE"
     gene            453..1787
                     /gene="cadB"
                     /locus_tag="ACHJ69_004480"
     CDS             453..1787
                     /gene="cadB"
                     /locus_tag="ACHJ69_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO5017313.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSIDNHTASNAH"
     gene            1867..4014
                     /gene="cadA"
                     /locus_tag="ACHJ69_004481"
     CDS             1867..4014
                     /gene="cadA"
                     /locus_tag="ACHJ69_004481"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO5017314.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAADDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            4073..5530
                     /gene="dtpC"
                     /locus_tag="ACHJ69_004482"
     CDS             4073..5530
                     /gene="dtpC"
                     /locus_tag="ACHJ69_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO5017315.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSCHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISWLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            5767..7284
                     /gene="lysS"
                     /locus_tag="ACHJ69_004483"
     CDS             5767..7284
                     /gene="lysS"
                     /locus_tag="ACHJ69_004483"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO5017316.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPLK"
BASE COUNT         1802 a         1693 c         1780 g         2093 t
ORIGIN      
        1 accctttact ggattgaaaa tggtatattc cagacttctg ttccttatgt tgtaccttat
       61 ctcgacaaat ttctcgcttc agaataagta attcccgggt tgattcatgc tcgggaatat
      121 ttgttgttga gtttttgtat gttcccgttg gtataatatg ttgcggcaat ttatttgccg
      181 cataattttt attacataaa tttaaccaga gaatgtcacg caatgcattg taaacattga
      241 atgtttatct tttcatgata tcaacttgcg attctgatgt gttaataaaa aacctcaagt
      301 tctcacttac agaaactttt gtgttatttc acctaatctt taggattaat ccttttttcg
      361 tgagtaatct tagcgccagt ttggtctggt caggaaatag ttatacatca tgacccggac
      421 tccaaattca aaaatgaaat taggagaaga gcatgagttc tgccaagaag atcgggctat
      481 ttgcctgtac cggtgttgtt gccggtaata tgatggggag cggtattgca ttattacctg
      541 cgaacctagc aagtatcggt ggtattgcta tctggggttg gattatctct attattggtg
      601 caatgtcgct ggcatatgta tatgcccgac tggcaacaaa aaacccgcaa caaggtggcc
      661 caattgcgta tgccggagaa atttcccctg catttggttt tcagacaggt gttctttatt
      721 accatgctaa ctggattggt aacctggcaa ttggtattac cgctgtatct tatctttcca
      781 ccttcttccc agtattaaat gatcctgttc cggcgggtat cgcctgtatt gctatcgtct
      841 gggtatttac ctttgtaaat atgctcggcg gtacctgggt aagccgttta accactattg
      901 gtctggtgct ggttcttatt cctgtggtga tgactgctat tgttggctgg cattggtttg
      961 atgcagcaac ttatgcagct aactggaata ctgcggatac cactgatggt catgcgatca
     1021 ttaaaagtat tctgctctgc ctgtgggcct tcgtgggtgt tgaatccgca gcagtaagta
     1081 ctggtatggt taaaaacccg aaacgtaccg ttccgctggc aaccatgctg ggtactggtt
     1141 tagcaggtat tgtttatatc gctgcgactc aggtgctttc cggtatgtat ccgtcttctg
     1201 taatggcggc ttccggtgct ccgtttgcaa tcagtgcttc aactatcctc ggtaactggg
     1261 ctgcgccact ggtttctgca ttcaccgcct ttgcgtgtct gacttctctg ggctcctgga
     1321 tgatgttggt aggccaggca ggtgtgcgtg ccgctaacga cggtaacttc ccgaaagttt
     1381 atggtgaagt cgacagcaac ggtattccga aaaaaggtct gctgctggct gcagtgaaaa
     1441 tgactgccct gatgatcctc atcactctga tgaactctgc cggtggtaaa gcatctgacc
     1501 tgttcggtga actgaccggt atcgcagtac tgctgactat gctgccgtac ttctactctt
     1561 gcgttgacct gattcgtttt gaaggcgtta acatccgcaa ctttgtcagc ctgatctgct
     1621 ctgtactggg ttgcgtgttc tgcttcatcg cgctgatggg cgcaagctcc ttcgagctgg
     1681 caggtacctt catcgtcagc ctgattatcc tgatgttcta cgctcgcaaa atgcacgagc
     1741 gccagagcca ctcaatagat aaccacacag cgtctaacgc acattaatta aaagtatttt
     1801 ccgaggctcc tcctttcatt ttgtcccatg tgttgggagg ggcctttttt acctggagat
     1861 atgactatga acgttattgc aatattgaat cacatggggg tttattttaa agaagaaccc
     1921 atccgtgaac ttcatcgcgc gcttgaacgt ctgaacttcc agattgttta cccgaacgac
     1981 cgtgacgact tattaaaact gatcgaaaac aatgcgcgtc tgtgcggcgt tatttttgac
     2041 tgggataaat ataatctcga gctgtgcgaa gaaattagca aaatgaacga gaacctgccg
     2101 ttgtacgcgt tcgctaatac gtattccact ctcgatgtaa gcctgaatga cctgcgttta
     2161 cagattagct tctttgaata tgcgctgggt gctgctgatg atattgctaa caagatcaag
     2221 cagaccactg acgaatatat caacactatt ctgcctccgc tgaccaaagc actgtttaaa
     2281 tatgttcgtg aaggtaaata tactttctgt actcctggtc acatgggtgg tactgcattc
     2341 cagaaaagcc cggtaggtag cctgttctat gatttctttg gtccgaacac catgaaatct
     2401 gatatttcca tttcagtatc tgaattgggt tctctgctgg atcacagtgg tccacacaaa
     2461 gaagcagaac agtatatcgc tcgcgtcttt aacgcagacc gcagctacat ggtgaccaac
     2521 ggtacttcca ctgcgaacaa aattgttggt atgtactctg ctccggcagg cagcaccatt
     2581 ctgattgacc gtaactgcca caaatcgctg acccacctga tgatgatgag cgatgttacg
     2641 ccaatctatt tccgcccgac ccgtaacgct tacggtattc ttggtggtat cccacagagt
     2701 gaattccagc acgctaccat tgctaagcgc gtgaaagaaa caccaaacgc aacctggccg
     2761 gtacatgctg taattaccaa ctctacctat gatggtctgc tgtacaacac cgacttcatc
     2821 aagaaaacac tggatgtgaa atccatccac tttgactccg cgtgggtgcc ttacaccaac
     2881 ttctcaccga tttacgaagg taaatgcggt atgagcggtg gccgtgtaga agggaaagtg
     2941 atttacgaaa cccagtctac tcacaaactg ctggcggcgt tctctcaggc ttccatgatc
     3001 cacgttaaag gtgacgtaaa cgaagaaacc tttaacgaag cctacatgat gcacaccacc
     3061 acctctccgc actacggtat cgtggcgtcc actgaaaccg ctgcggcgat gatgaaaggc
     3121 aatgcaggta aacgtctgat caatggttcc attgaacgtg cgatcaaatt ccgtaaagag
     3181 atcaaacgtc tgagaacgga atctgatggc tggttctttg atgtatggca gccggatcat
     3241 atcgatacga ctgaatgctg gccgctgcgt tctgacagca cctggcacgg cttcaaaaac
     3301 atcgataacg agcacatgta tcttgacccg atcaaagtca ccctgctgac tccggggatg
     3361 gaaaaagacg gcaccatgag cgactttggt attccggcca gcatcgtggc gaaatacctc
     3421 gacgaacatg gcatcgttgt tgagaaaacc ggtccgtata acctgctgtt cctgttcagc
     3481 atcggtatcg ataagaccaa agcactgagc ctgctgcgtg ctctgactga cttcaaacgt
     3541 gcgttcgatc tgaacctgcg tgtgaaaaac atgctgccgt ctctgtatcg tgaagatcct
     3601 gaattctatg aaaacatgcg tattcaggaa ctggctcaga atatccacaa actgattgtt
     3661 caccacaatc tgccggatct gatgtatcgc gcatttgaag ttctgccgac tatggtaatg
     3721 actccgtatg ctgcgttcca gaaagagctg cacggtatga ccgaagaagt ttacctcgac
     3781 gaaatggtag gtcgtattaa cgccaatatg atccttccgt atccgccggg agttcctctg
     3841 gtaatgccgg gtgaaatgat caccgaagaa agccgtccgg ttctggagtt cctgcagatg
     3901 ctgtgtgaaa tcggcgctca ctatccgggc tttgaaaccg atattcacgg tgcataccgt
     3961 caggctgatg gccgctatac cgttaaggta ttgaaagaag aaagcaaaaa ataattagct
     4021 cgtacaaggg aagtggcttg ccacttccct tttttgctca taaggagaac acatgaaaac
     4081 accctcacag ccgcgcgcga tatactatat cgtggcgatc caaatctggg agtacttcag
     4141 tttttacggc atgcgtgcct tactcattct ctatctcacc catcagcttg gttttgatga
     4201 taaccatgcc atcagcctgt tcagcgcata tgcttctctg gtttatgtta cccctattct
     4261 cggcggctgg cttgccgacc gcctgctcgg caaccgcact gcagtgattg ccggcgcgct
     4321 gttaatgacc cttggccatg tggtgctggg cattgataca aattcaacct ttagcctgta
     4381 tctggcgctg gcaatcatta tttgtggcta cggtttattc aaatcaaaca tcagctgttt
     4441 gcttggcgag ctctacgacg agaacgatca tcgacgtgat ggcggttttt cgctgctgta
     4501 tgctgcgggc aatatcggtt ctatcgccgc gcccatcgcc tgcggcctgg ctgctcagtg
     4561 gtatggatgg catgttggct ttgcccttgc gggaggcggc atgtttatcg gtttgttgat
     4621 tttcttaagt tgtcatcgtc atttccagtc cacgcgtagt atggataaaa aagcgctcac
     4681 aagcgtcaaa tttgccttac cagtatggag ctggttagtg gtgatgctct gtttagcccc
     4741 agtatttttt accctgctgc tggagaacga ctggtccgga tatttgctgg cgatcgtttg
     4801 cctcattgcc gcacaaatca ttgcccgcat gatgatcaaa ttccccgaac accgccgtgc
     4861 tctttggcaa attgtattgt tgatgtttgt cgggacgttg ttctgggtac tggcacaaca
     4921 gggcggcagt accatcagct tgtttatcga tcgctttgtg aatcgtcagg cattcaatat
     4981 tgaagtacct acagcactat tccagtcggt gaatgctatt gcggtgatgc tcgctggggt
     5041 tgttctggcc tggctggcgt cgccagaaag ccgcggcaat tcagcattgc gcgtctggct
     5101 gaagtttgcc tttggcttac tgctgatggc ttgtggcttt atgttgctgg catttgatgc
     5161 ccgacatgca gcggctggcg gtcaagcgtc aatgggcgtg atgatatcct ggctggcgct
     5221 aatgggcttt gctgaactct ttattgaccc ggtggcgatt gcgcaaatca cgcgtctgaa
     5281 aatgtctggc gtattaactg gtatttatat gctggcaaca ggcgcagtcg ccaactggct
     5341 ggcaggcgtc gtggcacagc agacgacaga gtcgcaaatt agcggtatgg caattgccgc
     5401 ttaccagcga ttcttttctc agatgggaga gtggacgttg gcttgtgtcg cgatcatcgt
     5461 ggtattggcc tttgctaccc gttttctgtt tagcacgccg acgaatatga tacaggagag
     5521 caacgattaa ctgtcgtaaa aacaattggt tatgaataaa ttaactttca gtttatcaca
     5581 ctagcaaatc gagattaata agtcatttaa accattttta tggttattta ttagtgatat
     5641 caacttgagg taaacgttag tttcgataag ataaactgag ttattaatag tcgaggcaga
     5701 taatacagtg taccgatctg tctctttatc tacgctaaat tgaaagctgg atttagagga
     5761 accaaaatgt ctgaacgaga aacacgggga gccaatgagg ctattgattt taacgatgaa
     5821 ctgagaaatc gccgcgaaaa actggcggca ctacgtcagc aaggtgtggc gtttcccaat
     5881 gattttcgcc gcgaccatac ctctgaccag ttgcacgaag agtttgatgc gaaggataac
     5941 caggaactgg aatccttaaa cattgaagtc tcggttgctg gccgaatgat gacccgtcgt
     6001 attatgggga aagcctcctt tgtaacgttg caggatgtcg gtggccgtat tcaactgtac
     6061 gttgcaagag atagcctgcc agaaggtgtt tataacgatc agtttaaaaa atgggatctg
     6121 ggtgacatta tcggtgcccg cggtacgctg tttaagacgc aaacgggtga gctttccatt
     6181 cactgtactg agctgcgcct gctgactaaa gcactacgtc ctttaccaga taaattccat
     6241 ggtctgcagg atcaggaagt ccgttatcgt caacgttatc tggacctcat cgctaacgat
     6301 aaatcccgtc aaacgtttgt tgtccgttca aaaattctgg ccgctatccg tcagttcatg
     6361 gtcgcacgcg gctttatgga agtagaaacc ccgatgatgc aggtaattcc aggtggggca
     6421 tctgctcgcc cgtttattac ccatcataac gctctggatt tagatatgta cctgcgtatc
     6481 gcgccggagc tgtatctgaa acgtctggtt gtaggcggtt ttgaacgggt attcgaaatc
     6541 aaccgtaact tccgtaatga aggtatttct gttcgccata atcctgagtt cacaatgatg
     6601 gaactctaca tggcatatgc ggattaccat gatttgattg aactgacgga gtcactgttc
     6661 cgcaccctgg cacaagaggt actgggtacc actaaagtca cttatggcga gcatgtgttt
     6721 gatttcggca aaccgtttga aaaactcacc atgcgcgaag caatcaaaaa atatcgtcca
     6781 gaaaccgata tggcggatct ggataatttt gatgctgcta aagcattagc tgaatctatc
     6841 ggtattacgg tagagaaaag ctggggactg ggacgtattg ttacggagat ctttgatgaa
     6901 gtggcagaag cacatctgat tcagccaacc tttattacgg aatatccggc agaagtgtcc
     6961 ccgctggcgc gccgtaatga tgttaacccg gaaatcaccg accgttttga attcttcatc
     7021 ggtggtcgtg aaattggtaa tggttttagc gaattaaacg acgcagaaga tcaggctgaa
     7081 cgtttccagg aacaggttaa cgctaaagct gcaggtgacg acgaagccat gttctatgac
     7141 gaagattacg tgactgcgct ggaatatggt ctgccgccaa ccgctggtct gggtattggt
     7201 atcgaccgaa tgattatgct gtttacaaac agtcatacta ttcgcgacgt tattctcttc
     7261 ccggcgatgc gcccactgaa ataaatttca ctttaatgaa cgaagcagtc aggcgactgc
     7321 ttcgttcgtc attccgcaaa tccagttttg aggagagacg cttcgttt
//
LOCUS       DAZFFE010000166         7336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFFE010000166 DAZFFE010000000
VERSION     DAZFFE010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.167"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..189)
                     /locus_tag="ACHJ69_004484"
     CDS             complement(<1..189)
                     /locus_tag="ACHJ69_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO5017317.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCED"
     gene            complement(284..472)
                     /locus_tag="ACHJ69_004485"
     CDS             complement(284..472)
                     /locus_tag="ACHJ69_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO5017318.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYDSKSSCIQVRDEQKIPGE
                     CLPLKKVSLNLNNEIPAG"
     gene            complement(469..657)
                     /gene="dicB"
                     /locus_tag="ACHJ69_004486"
     CDS             complement(469..657)
                     /gene="dicB"
                     /locus_tag="ACHJ69_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO5017319.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLELGIIGEQQ"
     gene            1233..1427
                     /locus_tag="ACHJ69_004487"
     CDS             1233..1427
                     /locus_tag="ACHJ69_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017320.1"
                     /translation="MSEQELKLGACYCVLKELVHMLPSTQYQQLVGNLNQRIEAMLKS
                     DGFNNVETLMLKRYLDGLIR"
     gene            complement(1416..1754)
                     /locus_tag="ACHJ69_004488"
     CDS             complement(1416..1754)
                     /locus_tag="ACHJ69_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017321.1"
                     /translation="MEFKDLPPSIQEIAAHTLRHRLNELELESVTKKDTDNMARNVRD
                     AFTGLFSDTALNIHGTEDTAKQFAEKEVEDPSSKSQKQPNNHEINELLLATGFININE
                     YERRKKMLSD"
     gene            complement(1766..1918)
                     /locus_tag="ACHJ69_004489"
     CDS             complement(1766..1918)
                     /locus_tag="ACHJ69_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO5017322.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTRSKTFKTEAGARRW
                     LTRNTD"
     gene            complement(2193..2483)
                     /locus_tag="ACHJ69_004490"
     CDS             complement(2193..2483)
                     /locus_tag="ACHJ69_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5017323.1"
                     /translation="MLPILWLPSARDDLRQIVAYIAKENPPAARRLKIRIETSVLSLT
                     EHPYLYPPSERALGLREIVTHPNYIILYRVTASNIEIVNVVHSRRQYPNKSC"
     gene            complement(2483..2674)
                     /locus_tag="ACHJ69_004491"
     CDS             complement(2483..2674)
                     /locus_tag="ACHJ69_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFO5017324.1"
                     /translation="MSTVLSPIISEFETIEQENSYNEWLRTKVAASLADPRPAIPHDE
                     VMAEMENLIAQIASTNRSE"
     gene            complement(2692..3192)
                     /locus_tag="ACHJ69_004492"
     CDS             complement(2692..3192)
                     /locus_tag="ACHJ69_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5017325.1"
                     /translation="MKRKPLSEIDLQAAQRLKEIWTTKKTQLGLTQERAAEILGFSTQ
                     GAVSHYLNGQTPLNLEAVIKFAGLLQVPPESIRPDMADLLRIVRMYPQELQQDNVVAL
                     PAEMEQSENELPFNIEPIERDLIQTFRAFPKEDKEKMLKEMKEKKESIEETVARWLEA
                     QKGLRA"
     gene            3300..3563
                     /locus_tag="ACHJ69_004493"
     CDS             3300..3563
                     /locus_tag="ACHJ69_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5017326.1"
                     /translation="MNLGEYLHHSRITQKDFAEIVGVTQGMVSHVITGRAKLTGGKVL
                     RWCEATGWVVTPHEIDSSTYPNPTDGIPVDYQANTQPALGVDS"
     gene            3560..3985
                     /locus_tag="ACHJ69_004494"
     CDS             3560..3985
                     /locus_tag="ACHJ69_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO5017327.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQINRGGPAGNAVVMH"
     gene            4008..4961
                     /locus_tag="ACHJ69_004495"
     CDS             4008..4961
                     /locus_tag="ACHJ69_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5017328.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     EAYKPKRKISPAKNDPSNIAPSTVGPSNVDGSTVDKKLRIRGAMIDPDPSVLKPDPSD
                     KRSSCPDVSLPDEKQSSPVERFLEKHPDAHTWNVPKRQWGTREDLTCAQWIWGRVVAL
                     YEQAASDDGEVSRPREPNWTTWANDVRMMRMLDGRSHRQICEMFGRVQRDSFWVKNIM
                     SPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            4968..5708
                     /locus_tag="ACHJ69_004496"
     CDS             4968..5708
                     /locus_tag="ACHJ69_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5017329.1"
                     /translation="MKNITSGGVLARVSRFVPQDAIPPYRTVAEWREWQLAEGRKRSE
                     EVNRLNHQTRVEKIINRSGIQPLHRKCTFGNYRVQNDGQRHALSQAKSIAAELEGGCT
                     NFVFSGRPGTGKNHLAAAIGNHLLAKGRSVIVITVADVMLALHGSYDNKNSGEKFLQG
                     LCGVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASLLSVGMLTNLNHAAMNTLLGERV
                     MDRMSMNGGRWVTFNWESWRPNVSQHRN"
     gene            5738..6463
                     /locus_tag="ACHJ69_004497"
     CDS             5738..6463
                     /locus_tag="ACHJ69_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO5017330.1"
                     /translation="METVFDALKALKRASSQVVAARLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPEAASVAETDGKAFPQPAGVALPVREAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            6479..6871
                     /locus_tag="ACHJ69_004498"
     CDS             6479..6871
                     /locus_tag="ACHJ69_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO5017331.1"
                     /translation="MAKVFTPEEREEVKARIVEFVRLSGRETFRQLADKTGVSKTAIR
                     RLSGALAASGDVWLSGCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYNRRQN
                     IICRESRRSEVMQRVLAFYRGNFQEVME"
     gene            6868..6963
                     /locus_tag="ACHJ69_004499"
                     /pseudo
     CDS             6868..6963
                     /locus_tag="ACHJ69_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309540.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
BASE COUNT         1871 a         1600 c         1970 g         1895 t
ORIGIN      
        1 atcctcgcag acaagaaatt tataagcgca gtcctgagca gccggataat gctccaggaa
       61 ttgccagtga aattttgcgc gggcgcgacg ttcatcaccg gcttcaatgg cagtggctac
      121 agcgacggca ccttcttcct ttattgcctg ttcgtccgga atggcggcgc aaataaagac
      181 tttactcatt ttgttttacc tcattacaga tttcagggtg aacgaatccc tgccattgct
      241 ggcattttta atccgttggt atggcgttaa tatggctggc gggttatcca gccggtattt
      301 cgttattcag gttcagcgat acttttttta acgggaggca ttcaccgggg attttttgtt
      361 cgtcccttac ctgaatgcag gatgacttac tgtcataaat tccggtaatc acattttgtg
      421 gctcacccgt tataagaaaa acggtcatca ccagtgcaaa tgctgaagtc actgctgttc
      481 tccgataata ccaagttcaa gaagggcaat tctggaaagt atggaattat cattgagaag
      541 ataaggttca tattttctca tcttaatggc atcttccgta aactcccggt tactgagcag
      601 aacaccaata tcaaaacaac cttcagacgt attaacgttt ggtaataacg tttccattat
      661 cgcgtcctca acaatgaatt ttgtgatgcg gtgcctggtg cctccaggtg acgttaacca
      721 gttaacaatt aacgccggat acagagcatt cccgttacgc ccgtaaaaga ccgcttgttt
      781 taactgttcc gcgtgcgctg agccgcattc accgcatcac aaaattcact ttaaaaaggg
      841 cggcagagca gtcacggagt aaaactgata ccgccaaatg tcaccagaaa attgataaca
      901 gagggcgttg tagcggggtt gtcacttaag cgtatggtca acctgacaac ccggtgcatt
      961 ttctggagca atggaggaaa ccccagccat acttaccgcc gcgccatttc gcggattgcc
     1021 acaaccggaa gcgcacggtc gacgaaaatt taacgacagg ctatctatga accagctacc
     1081 tcgccgtgcg ctttcgcgtt atggtctgac ttttcaggga aatatccttt cagtaaactg
     1141 tcagtgccgg attcttatcc gtgtccggcg cacgaccaca cgctgtcacg agaggtctcc
     1201 attctcaacc agtaacctca atggaggata aaatgtcaga gcaggagtta aaacttggtg
     1261 catgttattg tgttctcaag gagctggtac acatgcttcc atctactcag tatcaacagt
     1321 tagttggcaa tttaaatcag cgaatcgaag ctatgttaaa atctgatggt tttaataacg
     1381 tagaaacgct gatgctaaaa agatatcttg atggattaat cagataacat ttttttacgg
     1441 cgttcatatt cgttaatatt tatgaagcct gttgccagaa gcagttcgtt aatttcatgg
     1501 ttgtttggct gtttctggct ctttgaactg ggatcttcca cttctttttc tgcaaattgt
     1561 tttgctgtat cctctgtgcc atggatattt aaagctgtat ctgaaaacag cccagtaaac
     1621 gcatcgcgca cattacgagc catattatca gtgtcttttt ttgttaccga ttccaattca
     1681 agttcgttca gacgatgacg aagtgtgtgt gctgcaatct cctggattga aggaggtaaa
     1741 tctttaaatt ccatcgtcaa cctcatcagt cagtgtttct ggttaaccag cgacgcgcgc
     1801 cagcttcagt tttaaacgtt ttgcttctgg tatacgtcat cgcggtaaac gtaccgtcct
     1861 ggttggggaa cacgccacat accagagatt cgctgttgcc aagatcgata gtatccatgc
     1921 tgacctcatt tccccttaac gccggggtag cggaacaaaa acctgctgca tagttattaa
     1981 agttgaaccc tgccgtcatg ttcttacgcc tcgggctggc tacttacccc ctgaccactg
     2041 cctggtaact cgaagtattg ccctgcgttc tgtggggagg gtgggtgatg aataaacaat
     2101 agcactgcta ttttattctg taaatagtaa tgctattgtt ttttggaggt aagaaaaaaa
     2161 aaccacccga aggtggttgt tggtaggaat gattaacagc ttttgtttgg atactgcctt
     2221 cgtgagtgaa ctacatttac gatctcgatg ttagatgctg ttactcggta aagtattatg
     2281 tagttaggat gggtcactat ctcacgaagg cctagagctc tttcgcttgg tggatacagg
     2341 taagggtgtt cagtaagtga caaaactgat gtttcaatgc gtatttttag tctacgtgca
     2401 gcgggagggt tttccttggc aatataggct acgatctgac gcaaatcatc acgcgcagaa
     2461 ggtagccata aaatgggcag cattactcac tcctgttcgt cgaagctatt tgcgcaataa
     2521 ggttttccat ttcagccatt acttcgtcat gcggaattgc aggacggggg tctgcgaggc
     2581 ttgctgccac tttggtgcgt aaccattcgt tatagctgtt ttcttgttcg attgtttcaa
     2641 attctgaaat tatcggggaa aggactgtac tcatgttcta acctcctcag attaggcgcg
     2701 aagacctttt tgcgcctcta gccaccgcgc aacggtttct tcaattgatt cttttttctc
     2761 cttcatttcc ttaagcattt tctctttgtc ttcttttggg aaagccctga acgtctggat
     2821 caaatctcgc tctataggtt ctatattaaa cggaagttcg ttttccgatt gttccatctc
     2881 tgcgggtaaa gcgacaacat tatcctgctg cagttcttgg gggtacatcc ttacaattcg
     2941 taacaagtct gccatatctg gtctaattga ctcaggcgga acttgtagca acccagcgaa
     3001 cttgataaca gcctctagat ttaaaggtgt ctgaccattt agataatggc ttactgcccc
     3061 ttgtgtcgaa aaacccagaa tttctgccgc acgctcttgg gttaacccaa gttgagtttt
     3121 tttcgtcgtc cagatttctt tcagtctctg ggcggcttgc aggtcgatct ctgacagggg
     3181 ttttcttttc ataccttcaa ttctaataag attattaatc tctttgaaat agcagtgcta
     3241 tttactttta aaaataacaa tgctattaat attcatggtc acataacatg aggtgaacaa
     3301 tgaatcttgg agaatatttg catcattccc gtataaccca gaaagatttt gctgaaattg
     3361 ttggggtaac ccaagggatg gtaagccatg ttattactgg acgggcgaaa cttacggggg
     3421 ggaaagtttt acgctggtgt gaagcaacag ggtgggtagt gaccccgcac gagattgata
     3481 gcagtactta ccccaaccca accgatggca tacctgttga ctatcaggct aacacacaac
     3541 ccgcgctggg agttgattca tgaaaatcaa acatgaacac atccgcatgg cgatgaatgc
     3601 ctgggcatat cctgatggtg agaaagtgcc tgcagctgaa atagcccgga cttatttcga
     3661 acttgggatg acgttcccgg aactgtacga cgacagccat ccggaagccc tggcccgtaa
     3721 tacccagaaa attttccgct gggtggagaa ggatacgcct gatgctgttg aaaaaattca
     3781 ggcgctgtta ccggcgatcg aaaaggcgat gccgcctttg ctggtggccc gtatgcgcag
     3841 tcacagttct gagtattacc gggagattgt cgaacggcgg gatcggctgg tgaaggatgt
     3901 cgacgatttt gttgcgtcag cggtcgtttt gtatgaccag ataaatcgcg gcggcccggc
     3961 agggaatgct gtggtgatgc actaaaagca cggtgttcgg gggttttatg agcagcaagc
     4021 ttcatggtct tgtctgggaa gggtgcgcct tcaccggcat gatcttatcc agggtggcag
     4081 ttatggcccg tcttgcagac tacagcaatg acgagggcgt gtcatggcct gccattgaaa
     4141 ctatccggcg tcagatcggt gcaagaagtg aatccaccgt taaatctgct attgcagaac
     4201 tggcgaaaga gggctggctg acgaaggaag agcgtaaggt cggtgggcgt aacgtaagca
     4261 atatctatcg gcttaatgtg gaaaaacttg aagcagctgc agcggcggcg cgtgaggcat
     4321 ataaaccaaa aagaaaaatt agcccggcaa aaaatgaccc gtcaaatatt gccccctcaa
     4381 cggttggccc gtcaaatgtt gatggatcaa ctgttgataa aaaactgcgg attagggggg
     4441 cgatgattga ccccgatccg tcagtattaa aacctgatcc gtcagataaa agatcttctt
     4501 gtccagacgt ttcactgccg gacgaaaaac aatcatcacc agttgagcga tttctggaga
     4561 aacacccgga tgcgcatact tggaatgtac cgaagcgaca gtggggaacc cgggaggatt
     4621 tgacgtgtgc acagtggatc tggggacggg ttgttgcgtt gtatgaacag gccgccagtg
     4681 atgatgggga ggtatcacgc cccagagagc ctaactggac gacctgggcg aatgatgtgc
     4741 gcatgatgcg tatgctggat ggacgtagtc accgacagat ttgtgaaatg tttgggcgtg
     4801 ttcaacggga ttcgttctgg gtaaaaaaca tcatgagtcc ggcaaaactc cgggaaaaat
     4861 gggatgaact ggtaattcgc ctggggcgtt cgcccgcgca gcgttgcgtg aatcatattt
     4921 ctgaaccgga taccgaaatt ccgccggggt tcagggggta gcgcatcatg aaaaacatta
     4981 cgtcaggtgg tgttctggca agagtcagca gatttgtgcc gcaggatgca atccctccgt
     5041 accgtacggt ggcggagtgg cgggaatggc agcttgctga agggcgtaag cgaagcgagg
     5101 aggttaatcg tctgaatcat cagacgcggg ttgaaaaaat cattaaccgt tccggtatcc
     5161 agccgcttca ccggaagtgt acgttcggta actaccgggt gcagaacgat ggtcagcgcc
     5221 atgccctgag tcaggcgaaa tccattgcgg cagagctgga aggcggctgt acgaattttg
     5281 ttttcagtgg caggcctggc acaggaaaaa accacctggc ggcggctatt ggcaaccacc
     5341 ttctggcgaa aggtcgcagt gtgattgtga taacagtggc ggatgtgatg ctagcgttac
     5401 atggcagcta cgacaacaaa aactcgggcg aaaaattttt gcagggattg tgtggcgttg
     5461 acctgctggt actggatgaa attggcatgc aacgggatac gcgtaatgag caggtcacgc
     5521 tgaatcagat tgttgaccgt agaacggcat cgttactcag tgtggggatg ctgacaaatc
     5581 ttaatcatgc agcgatgaat acacttctcg gcgagcgggt aatggaccgc atgtccatga
     5641 acggtggtcg ctgggtgacg tttaactggg agagctggcg tccgaacgtc agccagcaca
     5701 ggaactgaga agtaattttt atccggagga aattttaatg gaaaccgtat ttgacgcact
     5761 gaaagcactg aaaagagcct cttcacaggt agtggcggcc cgccttggaa tcagccgtga
     5821 agatgcggtc aacgaactgt ggaaactgaa gcgccgcggt gaagcggata acaagggttc
     5881 gatgtggtgg ctgattcagg ctggtgaaag tgaaccggtg tcaccggtac cgaaagtgac
     5941 agcgcaaatg ttgactgagg cgattgaaca acatggccca caaacggcgg atgagctggc
     6001 actgatgttc gggattacct cccgccgggc gaattcatcg ctggccatgg caatcagcaa
     6061 agggcgtctg attcgcgtga atcagggcgg taaatttcgt tactgcatac cgggcgctga
     6121 tttaccggca gagccggaag ctgcatccgt agcggaaacc gatggtaaag cctttcctca
     6181 gccagcaggt gttgcgttac ctgtccggga agcagaaaca caggaagaaa taaaaacgga
     6241 aagtgtggcg gtcacagtgc agtcacagcc gtcgttcacc agaaaacatc cggatggtct
     6301 gattttacca tcgctgcatg tggctaaccg cgagctgcgc cgggcaaaag gtcaggttca
     6361 gaagtgggag cgagtctgcg ccgcgctgcg ggagctgaac aagcaccggg atattgttcg
     6421 acagattgtc gattcatccg gtcgtattgt gtcggaaaag tgattgccgg aggcgcttat
     6481 ggcaaaagta tttacaccag aagagcggga agaagtgaag gcgcgcattg tggaattcgt
     6541 gcgcctgagc ggacgagaaa cttttcgaca actggcagat aaaacgggtg tcagtaagac
     6601 cgctattcgt cgtttatctg gtgcgcttgc ggccagtggt gatgtctggc tctctggttg
     6661 cggggtattt ccatcagagc aggcgtatcg cgtatggcgt aagacaccgg agaaggctgc
     6721 tgacccgaca ctgattcgaa agttacctga cggagaaata cgtcgttaca acagacggca
     6781 gaacataatt tgtcgtgaga gcaggaggag cgaagttatg cagcgtgtgc tggcgttcta
     6841 tcggggaaac tttcaggagg tgatggagtg agggtgagag tttatattgc cggtccaatg
     6901 acgggatatg aaaatttcaa ccgcgaggcg tttcacaggg cggaagatgc gctggctcgt
     6961 aaagcagttc aggcattttg cgatgttgtt ggcgacagca ccgaggttat ctgcgaggag
     7021 attgggcgag atggcgttct ggttattttg gaggcaatga aggcaacagg aaatatgcca
     7081 gccactgatg ctttcctggc tgaagtccgg gcgcaggggg tggatgctgc tatagaagct
     7141 gcaaaaaatc tggtggccca agaatatgag tataaggatt tcaaagcggc gcagagtgat
     7201 tgctgtatgt accctggttc agacctggta gggaaggttg aaatgactga gtggttagtt
     7261 gactttgctg cccagcttcg caaaggaggc aaccagtgag cggaaaaaga atgactaaca
     7321 gagagcttgt cgatgc
//
LOCUS       DAZFFE010000167         7070 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFFE010000167 DAZFFE010000000
VERSION     DAZFFE010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.168"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(73..2856)
                     /locus_tag="ACHJ69_004500"
     CDS             complement(73..2856)
                     /locus_tag="ACHJ69_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO5017332.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEVLALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTFSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLPQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSNSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYNSRTPVNLATRMALDAFNVALAKDLRINIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2913..5285)
                     /locus_tag="ACHJ69_004501"
     CDS             complement(2913..5285)
                     /locus_tag="ACHJ69_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO5017333.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPESDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDISGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAARKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNRVVYNGNLVSYDSVPVTDFNNPLKISLNMDFT
                     HQPSGLVWANTLTWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5323..7008)
                     /locus_tag="ACHJ69_004502"
     CDS             complement(5323..7008)
                     /locus_tag="ACHJ69_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO5017334.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWSIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSENFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALSLSVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLVDDICDISAVI"
BASE COUNT         1844 a         1616 c         1579 g         2031 t
ORIGIN      
        1 ttataaaatt attacataaa aatagcgaat attgataaaa tccccgccaa cgatgtgttg
       61 acggggctgt gattattttg gcaataatac tccggtatag gtattcaccg gatgagaaag
      121 atattgttta acagcagtgt taacattctc taccgtcatt tgtttcaaca attgctcctg
      181 ctcagtccat gctgcaggat cgtcatattg aataagacta tttacaatgg tattcgctaa
      241 ttgttgaacg ctacgctgtt ggatatcgag gctgcgctga acgttttgct ggtattcgtt
      301 cagttcttgc tcactaatcc ctttggccag acgcttaacc atcacttcat tcgctaacgt
      361 taacagttca tcatgtcgtt ctggttgaca agtaaaagcc agcaaatgac tgatatcttt
      421 ggcctgagga tcaaccgaga ggcgagaaga aacgctgtat gccccagatg cctgttcacg
      481 aatattaatg cgtagatctt ttgccagtgc gacgttaaaa gcatcgagcg ccatacgcgt
      541 cgccagatta acaggagtcc gggaattata acgcttccac tgtgaaacct gtgccactgg
      601 ttcattttgt tcttttacag taaccgatgc gttgtccgtc gcgcgagtta atggtttacc
      661 tgcggctaat ggcgaattag agtgtttgat agatcctaag taacgcgtaa ttaacgccac
      721 gagtttgtct tctgcgacat taccgacaat gacaaacgtg atatccgctg gagatgaaaa
      781 caactggcga tcggcagcca gtgcatctgc ggcagtaaac tgtgcaatct gattttcttg
      841 cggtaatttc gtgcggtcat cagcatagcg cgtctcatac atctgctggg cgaatttctc
      901 cgctggacgc tggtcgagcg ttttcaacgc ctgaatttga gcattttgca gcgatgccca
      961 aatattgtcg ttaatcgtgc tgtgggtgat tcgctggtta atcaactgga aaccaggttc
     1021 agggttatta gtccgcgcgc taacggagag caacgtattc atgccactga ctttactgct
     1081 catggttacc gagttttccg cactccagcg tttcaggctg gaagaggaga gttcaccaac
     1141 gccgcttccg cttactgctt tattagccag cgcgataagt gatttttgct gcgcagggaa
     1201 acttaaatcg cctttattag atacggcaat aatttgtagc ttttgctctt cacccgcgga
     1261 ttttgccaga ataaccctgg caccattgga aaatgttaat gatgtcagat tctcagccag
     1321 cgtttctttg ctgctaattt ccgcctgtgg gtcagcgtct actgttaacg ataaatttct
     1381 gcctgggaag atgtaagccg ccagcttttt gttggcgtac tctttttcca gcgccaggat
     1441 agccgcagga gacaatgctt ttttggcggc aacttcattg tttaccattt gctcccaaaa
     1501 tgcgtcctgg ttctttctta actgctgcca tttttccgcc agactttgca cggtaatttg
     1561 ctgccacaga cgtttcgaaa gttgatatgt ctcttccggc gacaagaacg gcgtattatt
     1621 taatgagctg gatgccaggc gactggtcag catacgtaaa tcacgctcag cttgctgatc
     1681 aaccgcattt ttcagccagg taaggcgggt agatttaaca tcatcgagtt cttcagcaga
     1741 aaaaccatgc tgatcaatgg ttgccaactc tgccattaat gcattcgcag catcctgcat
     1801 attatcgtct cgtgcattta cacggaaaaa tagcgactga taatcgggtg caattttgac
     1861 gctgcgcgca gtgccgccag aaatagtctt caactcgccc gactgtatgc gttcctgcag
     1921 acgttggttg aacagctgaa ccaacatgct ccattcagct tgttcgataa agctttgctc
     1981 atcgttcact tgtaccattg gcaggcgata atagagtgcg atgccgttca cccggttttc
     2041 tttatcattg atgatattaa agcgcaggtg gttttcggct tttgtcggcc agacgcgatt
     2101 ttcagctgct ttgttagccg gaagcttact taaattatcc tttatcagcg ccagcacttc
     2161 tttactgtcg atatcgccga ccacaataaa ggtcatatta tttggttgat accagcgttg
     2221 ataaaattgg cgcaattgtg ccggtgtgac cgtggcgacg gtatccatca ggccgatagg
     2281 ttcacggtct aaattacggg tatttgccag caggaaaggg cggcgcgcct gagaggtgcg
     2341 ccatttcgca tcctgatggg cacgccattc ctcagtaatt acgccgcgtt cagcgtctac
     2401 ttcgagtttt tcaaaggttg cggcattact ccattcactg aagattgcca tcacttgttg
     2461 cagattttgt ttctgcgtag tcggcaaact cacctgatac actgtttcgt catagctggt
     2521 ataggcatta acatcgcgac caaaacgcag gcccattgac tcaaatgttt cgatgacttt
     2581 attacccggc catgtttttg tgccgttaaa catcatatgt tctacaaaat gagccacgcc
     2641 gcgctcattg tcttcttcct gcaatgaacc ggtatgaatt tgcagccata aatttacctg
     2701 atcctttggt tgagcatgcg gataaatcat atatcgcaag ccattgtcca gttgcccggt
     2761 aattaacttt tcatcctgcg gtaaggcggc ggcaatcagt cgcccgggga gcaacagagt
     2821 tgccactaac gtcagtaaga aacagaggtt tctcataatt atctccatgc gaaaaccggg
     2881 cgaatttacc cggtaaagta aaatccgaac tattaaaatt tcatgctgac atcaagccag
     2941 aaagtacggc cactggcgta ggtcgccacg ccagtattcg ttgtatcaac agcggttttg
     3001 ctatccagta cattgagaat atcggcactg atcgtcaggt tttgttgttt cagaaattgt
     3061 ggcgtccagg acaagcgggt gtcccaggtc aggctgctat ccagtttctc gtcaacatac
     3121 tgcttgtaat ctgaatattc gctgatgtat tgcgcattcg tcttacccag gataatgcga
     3181 gctttacgcg cttcttgcca ggtcagcgta ttcgcccaca ccaacccgct cggttgatgc
     3241 gtgaaatcca tgtttaagga gatctttaat ggattattaa aatccgtcac tggaacgcta
     3301 tcgtaagaga ccagattacc gttataaacc acccggttat caccggtatt gctctcctca
     3361 taaccattgt ttaacgacaa gttgcccttg ctcttgatat agctaaagac aatttgcggg
     3421 ttaatatcta cctggctgat atgcaggggt tcggccagtt caaaactgag gttaaacgaa
     3481 tgtgttttgg ttttgccatc gttgttatat tcagtaatgg tggttttagt cgcgctgtcg
     3541 gtacgactgc ttttgctgat ttgatcatgc gcttcacggt aaacatagtt tgcacgcgca
     3601 ataacgttct taccgatttt ctgctgcaat cccattgcca gttcatcgtt ataaggcgtt
     3661 ttcaaatcct gataacgcgt cagggtttta ttgcctgata ccgattccgt ccagctattg
     3721 cggatatcac gtaatcccat atcaagaata ttcccgccat agtaacggtt ataacctgca
     3781 gtaatcattg aggtttgatc agcaaaaata tcccattccg tcataaagcg cggggagata
     3841 ttgtggtttg acagatagtt atcatagtca taccgcacgc cgggcattaa cgacacatta
     3901 cgccagctaa tgcgatccgc catatacagc gtgtaattgt caattcccag actaccttta
     3961 cctttatggt aaattgtatg gttagttttc tttctggcag cattaatcac ataggattca
     4021 gactggttat ggcgttcagt ccatgcatcg gaatagatgt attccgcgcc gaagtagggt
     4081 tgatgcgaaa cattacccac ggcgaatttt tgccagtcca ggcgtgtttt gaaggtgtaa
     4141 ttatctacag cctgggaaat gtgtcctaat ccgccacggg tgcaacgccc tgaaatatca
     4201 ccatatgtac atgaaagttc ggtgtaccag atgtcatgat cgtgacgggt ataatcacta
     4261 atatgatccc aaccaacggt ggtacgcagt ttggcccatg cgagctgggt atccatatcc
     4321 catgccagac cataggattt attacccatt tcgcgatcag actccgggaa ggtgctggta
     4381 ttataatcac ggctggagcc ggtatatttt aaggttaaat cgtgggtaaa gcggtcgctg
     4441 gcaaaccagg taaatttgct caatgcagta tcgataacgt ttttatactg tgccctgccg
     4501 gcgacaatgc cgtcattcga aacataatcc gcgcgggtga tatcagactg gcggcgcgat
     4561 aaaccagcgg taacgccaaa gttatcagcg agttcctgat taaacgacaa ggtataaaag
     4621 ttctttttaa aatctggaga gtaataagta cttcctgaag aaccttgatt aaatgcgctc
     4681 ttgttattct catcgatatg cgatgttaac cagtccgaac gcgtagtgcg ataacccagt
     4741 ttcacgctgc tatcatcagc gttgaagcgt ttgatctttg catcaattac cccgccattg
     4801 aagcgaccga attcaactgg cacaaaactg tcataaagcg tcacattgtc cagtaagctg
     4861 acatcaagat aatacccctg tgacatcccg ctaatattgg ttgcactact ggcatcggac
     4921 tcattcgctg ggttcaggtt attcgttgca ctaataccgt caatcaaata ggcattctgg
     4981 tagggcgacg caccgtgaat agagattttc tccgggcgaa tatctccctg gttcaatgag
     5041 gtactttgcg ttgaatccat gcgtaccgca gggttggttc tcagcagatc gctgatattg
     5101 ccattcccgg tcggtaaacg ctcgatgctt tcactggtgt aatgcgtgtt gccgtttacg
     5161 ggtacaggga caggagcata gaccgtcatc tcttcaaaaa catgcatgta catatttttg
     5221 tcatcgacgg cctgcgccac atcagcgcca cataaaatga cgccaggaat aagaactcgc
     5281 ttcatttatt tatatctccg tacagcaggg cttttattgt ttttatataa ccgcgctaat
     5341 gtcacaaata tcatcgacca ggttccagac accgggttga tgtgtaacca taatgacgcc
     5401 gcttgtgggg agtttttctc gcactaaacg cagtaagcgg attgcctctt gttcctcaag
     5461 atgagaggta gtttcgtcaa gaaatatcca tttcggacgt cgtaaaatta atcgagccag
     5521 ggcgatacgt tgtttttcgc cgctggaaag aatatctccc cagcgatcgt gatcatgaat
     5581 acgcgcagcc aatttcccca gaccaacctg atgcagtact tcgctcaacg atttatcgtc
     5641 tacggacagg gaaagtgctt tacaaataat ctctttcagt aagccggttt tgattaacgg
     5701 tgtttgtgac acataccaac tgtcagcagg agaagaaata tcacctttaa accacggcca
     5761 gcagtgggat aatgttttaa gcagtgtggt ttttcccgca ccagagtaac ctttcagcaa
     5821 tagccatttg cctggcgaaa catgaaagtt caggttctct aatatgatct tattatcagg
     5881 agtacgaata ctcgcgtcag ccacttgtac cgcatgttga caatttttag gcttattcgt
     5941 agggcgctgt tcggtgagtt gatggaactc atacaagcga tcgataaccg cagccagttc
     6001 agcaagttca tcatatttat aaataaacca gcttaaattg ttcgatacca gcataaatgc
     6061 ctggcgcgat ttcatcagtc cgcccagatt gatctgcccg ctaataaact gcggcaataa
     6121 cagaaagtag ggaagaacgc taagcgagcg cgaataaata ttctgccagt aatcaagcca
     6181 ccgctgacga ttcattaaac gatgccagtt ctctttaatg gtatgaaaat tttcgctcaa
     6241 ctcctggcgt tgtagagact cagcattgct tagcgcaatc agttctgcct gcttattatg
     6301 ctgcacaaga ttagttcgaa atgtcgcttc gctacgttgt ttttccacat taagtggacg
     6361 aatacgttta ccaaccttat gggtaaataa agttccaccg atcacaatga gcacgacggt
     6421 atagaccata tacccctgga tactccattc agttccacca acagtgaatg agagcgtacc
     6481 cgcgctttgc caaagaataa cggtaaaggt gatcagcatg ctaagtgact ggatgaagcc
     6541 aaatgaaagg ctgagcgttt tgctgatcag taagagaatg tcttcagcga tacgttggtc
     6601 agggttatct gtatttttat gctcgccgta gatctgcgtg aagtagtagt ttttatctgc
     6661 aaaccagcga ttaaggtagt aatctgttaa ccattcgcgc cagcgaatag tcagtagttt
     6721 aattaaccat gttttgttta cggatattaa tacaaagatc cccagcaggg cagggaacca
     6781 aaggacaagc tgccaaagct tatcagtttc tttctggctt agcgcattga aaaaatcatt
     6841 attccagtcg tttaaccaga cctgaatttt aaccacgcca aggatcatgg cgaggataat
     6901 aatgattaac aacactgaag ttttattatt cttacgcagc caaaagggct tcagcagaca
     6961 gagatacttt gctatcaaca tacaaaacgt aatgggaatg gttatcatta gcgaaaattg
     7021 attgtgccac ccaatgatta tctatggcaa tgatatactg atgaaattta
//
LOCUS       DAZFFE010000168         6978 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFFE010000168 DAZFFE010000000
VERSION     DAZFFE010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.169"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            206..1081
                     /gene="rluB"
                     /locus_tag="ACHJ69_004503"
     CDS             206..1081
                     /gene="rluB"
                     /locus_tag="ACHJ69_004503"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO5017335.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
     gene            complement(1121..1711)
                     /gene="cobO"
                     /locus_tag="ACHJ69_004504"
     CDS             complement(1121..1711)
                     /gene="cobO"
                     /locus_tag="ACHJ69_004504"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO5017336.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(1708..2466)
                     /locus_tag="ACHJ69_004505"
     CDS             complement(1708..2466)
                     /locus_tag="ACHJ69_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO5017337.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQDPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            2686..3735
                     /gene="sohB"
                     /locus_tag="ACHJ69_004506"
     CDS             2686..3735
                     /gene="sohB"
                     /locus_tag="ACHJ69_004506"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO5017338.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDTHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVVTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(3771..4022)
                     /locus_tag="ACHJ69_004507"
     CDS             complement(3771..4022)
                     /locus_tag="ACHJ69_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO5017339.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            4402..>6978
                     /gene="topA"
                     /locus_tag="ACHJ69_004508"
     CDS             4402..>6978
                     /gene="topA"
                     /locus_tag="ACHJ69_004508"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO5017340.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCSKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGK"
BASE COUNT         1772 a         1660 c         1891 g         1655 t
ORIGIN      
        1 gatgttaatc gaaacaagct ttaaccataa agatagtgta gtatgttgcg cctcgaagcc
       61 aggccagtta aaacgtcgag tcgtttactt aaggcctgaa gagttcaaac agactttact
      121 atcagtgagt cagatgtaag ttctgactca ttgttgcaca aaagcatatc agacgcctgg
      181 gaaggcgaca ccaaaaggaa gctctatgag cgaaaagcta cagaaagtgc tggcgcgtgc
      241 cggccacggt tctcgccgtg aaatcgaatc tattattgaa gccggtcgtg tgagtgttga
      301 tggcaaaatt gctaaactcg gcgatcgcgt tgaagttacc cctggattga aaatccgtat
      361 cgatggtcac ctgatttcgg tacgtgagtc cgctgaacaa atttgtcgcg ttctggccta
      421 ttacaaaccg gaaggtgagt tgtgtacccg taacgatccg gaaggacgtc caacggtgtt
      481 tgaccgtctg ccaaaactgc gtggcgcacg ctggattgcc gtgggtcgcc tggacgttaa
      541 tacctgtggt ctgctgctgt tcaccaccga tggtgaactg gcaaaccgtt taatgcaccc
      601 aagccgtgaa gttgaacgtg aatatgccgt gcgtgtattt ggtcaggttg acgacgcgaa
      661 actgcgtgat ttgagtcgtg gcgtgcagtt ggaagatggt ccggcagcct ttaaaaccat
      721 caagttcagc ggcggcgaag ggatcaacca gtggtacaac gttactctga ccgaaggccg
      781 taaccgtgaa gttcgtcgtc tttgggaagc ggttggtgtg caggtgagcc gcctgattcg
      841 tgttcgttac ggtgatatcc cactgccaaa aggtctgcca cgcggtggct ggacggagct
      901 ggacctcgcc cagactaact atctgcgcga actggtggaa ctaccgccag aaaccagctc
      961 taaagtcgct gtagaaaaag accgtcgtcg tatgaaggcg aatcagattc gtcgtgcggt
     1021 gaaacgtcac agtcaggtga gcggcggtcg tcgttctggc ggacgtaata ataacggtta
     1081 atcaaagtat gccggacgtc atatccggca tttttacaga ttagtaatca atccctatct
     1141 gcgcttttac accggcatca aacgcatgtt tgacggggcg taattcactt accgtatccg
     1201 ccagttcaag aatatcccga tgacaaccac gacctgtgat aatcaccgtc tgttgatgtg
     1261 gacgttcatt taacgcctgc accacttctt ccagtggcaa atagtcatac gccaccatat
     1321 acgtcagttc atcgagcaaa accatatcca gtgaggaatc agccagcatc cgctttgcat
     1381 gttgccagac ttcgcggcag gcggcggtat cagactctcg gttttgtgta tcccaggtaa
     1441 agcccgttgc cattacctga aactcaacgc cgtgtggctc cagcagattt cgttcaccat
     1501 taggccaggt gcctttaata aactgcacga cgcccacttt ttttccgtga ccaactgcgc
     1561 gtgttgccgt accaaatgcc gcggtggttt tgccttttcc attgccggta aagacgatga
     1621 taataccgcg ttcatcctgg gcctgggcca cacgagcatc tactttttct ttcactcgct
     1681 gctgacgctg ttggtagcgt tcatcactca ttgggaaatt cctggtttac ggcccggttg
     1741 ggcgtcaaag gtcatgccgg ttttacggcg gctgtcatcg cccatcagcc agaggtagag
     1801 cggcatgata tcagcggggg ttttaagttt ctgtggatct tcggtcggga aggcgctggc
     1861 ccgcattgcg gtgcgcgtac cgcctgggtt aatgcagttg acacgcaggc gctgctgata
     1921 ttcatcggcc agtacctgca tcatcccttc ggtggcaaat ttcgacgctg catatgcgcc
     1981 ccagtttgca cgcccctgac gtccaacgct tgatgaggta aagaccagtg aaccggcatc
     2041 cgatttgagt aataaaggaa gcagtgcctg tgtgagcata aaggtggcat taacgttgac
     2101 ctgcatgacg tcctgccaga cctgcggatc ttgttcgctc attgggcaaa catcgccgag
     2161 caatccggca ttatgcaaaa caccatccag acgcggataa ttaacggcaa tgcgctgtgc
     2221 cagttgttgg caatcttcgg acgtgcaggt tagcaaatcg agaataaacc actgtggctg
     2281 acgtccagtt tcttcgttta tgtggctggc tacctgacgt aatttttctt cattacggcc
     2341 caaaagaatc actgtcgcac catagcgtgc atacgtcatc gctgcttcac gaccaattcc
     2401 atcgctggct cccgtcacca ggataatgcg atcattgagt aaatcttgtt ttggctggta
     2461 atgcatggct actcctcaac gacgttgtct gtcgcgctgc cagtgtactt tatgactttg
     2521 gggctttatg cctgaaacag aagggcattt caatcagcct ggggcgataa caccgcaaaa
     2581 tggatacttt gtcatacttt cgctgcaata acatctctgc gagacggcta aacatgcctg
     2641 ttgtaaactg tgagccaaag cgttgtttaa ccaaggtggg gactcgtgga attgttgtct
     2701 gaatatggtt tgtttttggc gaaaatcgtt accgttgtgc tagcgattgc ggcgattgcc
     2761 gccattattg tcaatgttgc tcaacgtaat aaacgccagc gtggcgagtt acgagtcaac
     2821 aatctcagcg aacagtataa ggagatgaaa gaagaactgg ccgcggcgct gatggacact
     2881 catcagcaaa aacagtggca caaagcgcag aagaaaaagc acaagcaaga agcgaaagcc
     2941 gcaaaagcga aagctaaact gggcgaggtg gtaactgaca gtaaaccccg cgtctgggtg
     3001 ctggatttta aaggcagcat ggacgcccat gaagtgaact cgctacgtga agagataacg
     3061 gcggtactcg cagcattcaa accgcaggat caggttgtgc tccgtctgga aagcccaggt
     3121 ggcatggtgc atggttacgg gttggcggct tcgcagttgc agcgtctgcg tgataaaaac
     3181 attcctttaa ctgttacggt agacaaagtc gctgccagcg gcggttacat gatggcctgt
     3241 gtggcggaca aaattgtttc cgcaccgttt gctattgtgg gttccattgg ggtggtggcg
     3301 caaatgccca actttaaccg cttcctgaaa agcaaagata ttgatatcga actgcacacc
     3361 gccgggcagt ataagcgcac gctgacgttg ctgggtgaaa ataccgaaga agggcgggag
     3421 aaattccgcg aagagttgaa cgaaacgcat cagttattta aagattttgt gaagcgtatg
     3481 cgtccgtctc tggatattga acaggtggca acgggtgaac actggtacgg acaacaggca
     3541 gtagagaaag gcctggttga tgaaatcaac accagtgatg aagttattct tagcctgatg
     3601 gaaggccggg aagtggtcaa tgtacgctat atgcagcgta aacgactcat tgatcgattc
     3661 accggcagcg cggcagagag cgccgatcga ttgttgttac gctggtggca gcgtgggcaa
     3721 aagccattga tgtaaaagac aaacgcgagg gtaagacctc gcgttttgct ttaatcaacc
     3781 agatgatatt tttctgaaag cacatgggcc aggtgtttga acatattaaa caccgcggtg
     3841 cttttggcag ttggtaatcc ttgttcatct aaaaagtagt cgccggtaaa taccagcacg
     3901 ccattacgct gcgtgacgcc ggtggcttca atccctgcga gcgtatcctc atgctcacga
     3961 atgattttgt tggcctcttt caacagcgtt tcgcggtcga tgggttgtgt ctctttgttc
     4021 attatttact ccttaaacaa ggacattagt ctacgccagg catggcttgc agacaaatat
     4081 accacgctgg tggcaagagc gccttactgg caactttgga ttttgcatgc taataaagtt
     4141 gcgtatcgga ttttatcagg tacagtgtga cgctttcgtc aatctggcaa tagatttgct
     4201 tgacattcga ccaaaattcc gtcgtgctat agcgcctgta ggccaagacc tgttaactca
     4261 gtcacctgaa ttttcgtgaa cagagtcacg acaaggggtt gatatccgca gagagcgagt
     4321 ccatatcggt aactcgttgc cagtggaagg tttatcaacg tgcgacgcat tcctggaaga
     4381 atcaaattag gtaaggtgaa tatgggtaaa gctcttgtca tcgttgagtc cccggcaaaa
     4441 gccaaaacga tcaacaagta tctgggtagt gactacgtgg tgaaatccag cgtcggtcac
     4501 atccgcgatt tgccgaccag tggctcagct gccaaaaaga gtgccgactc tacctccact
     4561 aagacggcta aaaagcctaa aaaggatgaa cgtggcgctc tcgtcaaccg tatgggggtt
     4621 gacccgtggc acaattggga ggcgcactat gaagtgttgc ctggtaaaga gaaggtcgtc
     4681 tctgaactga aacaactggc tgaaaaagcc gaccacatct atctcgcaac cgaccttgac
     4741 cgcgaagggg aagccattgc atggcacctg cgggaagtga ttggtggtga tgatgcgcgc
     4801 tatagccgag tggtgtttaa cgaaattact aaaaacgcga tccgccaggc atttaacaaa
     4861 ccgggtgagc tgaatattga tcgtgttaat gcccagcagg cgcgtcgctt tatggaccgc
     4921 gtggtggggt atatggtttc gccgcttcta tggaaaaaga tcgctcgtgg tctgtctgcc
     4981 ggtcgtgtgc agtcggtggc ggtccgcctg gtggtcgagc gtgagcgtga aattaaagcg
     5041 ttcgtaccgg aagagttctg ggaagtcgat gccagcacga ccacgccatc tggtgaagcg
     5101 ttggcgttgc aggtgactca tcagaacgac aaaccgttcc gtccggtcaa caaagaacaa
     5161 actcaggctg cggtaagtct gctggaaaaa gcgcgctaca gcgtgctgga acgtgaagac
     5221 aaaccgacaa ccagtaaacc tggcgctcct tttattacct ctacgctgca acaagctgcc
     5281 agcacccgtc ttggttttgg cgtgaaaaaa accatgatga tggcgcagcg tttgtatgaa
     5341 gcaggctata tcacttacat gcgtaccgac tccaccaacc tgagtcagga cgcggtaaat
     5401 atggttcgcg gttatatcag cgataatttt ggtaagaaat atctgccgga aagtccgaat
     5461 cagtacgcca gcaaagaaaa ctcacaggaa gcgcacgaag cgattcgtcc ttctgacgtc
     5521 aatgtgatgg cggaatcgtt gaaggatatg gaagcagatg cgcagaaact gtaccagtta
     5581 atctggcgtc agttcgtagc ctgccagatg accccagcga aatatgactc cacgacgctg
     5641 accgttggtg cgggcgattt ccgcctgaaa gcacgcggtc gtattttgcg ctttgatggc
     5701 tggacgaaag tgatgcctgc actgcgtaaa ggcgatgaag atcgtatctt accggcagtc
     5761 gataaaggcg atgctctgac gctcgttgaa ctgacaccag cccagcactt taccaagcct
     5821 ccagcccgtt tcagtgaagc atcgctggtc aaagagctgg aaaaacgcgg tatcggtcgt
     5881 ccttctacct atgcgtcgat catttcgacc attcaggatc gtggctatgt gcgagtagaa
     5941 aatcgtcgtt tctatgcgga aaaaatgggc gaaatcgtca ccgatcgcct ggaagagaat
     6001 ttccgcgagt taatgaacta cgacttcacc gcgcagatgg aaaacagcct cgaccaggtg
     6061 gcaaatcacg aagcagagtg gaaagctgta ctggatcact tcttctcgga tttcacccag
     6121 cagttagata aagctgaaaa agatccggaa gagggtggta tgcgcccgaa ccagatggtt
     6181 ctgaccagca ttgactgtcc gacttgtggt cgcaaaatgg ggattcgcac agcgagcacc
     6241 ggggtattcc ttggctgttc tggctatgcg ctgccgccga aagagcgttg caaaaccacc
     6301 attaacctgg tgccggaaaa cgaagtgctg aacgtgctgg aaggtgaaga cgctgaaacc
     6361 aatgcgttgc gcgcaaaacg tcgttgctcg aaatgcggca cggcgatgga cagctatctc
     6421 atcgatccga aacgtaagtt gcatgtctgt ggtaataacc caacctgcga cggttacgag
     6481 atcgaagagg gtgaattccg cattaaaggt tatgacggcc cgatcgttga gtgtgaaaaa
     6541 tgtggctctg aaatgcacct gaaaatgggg cgtttcggta aatacatggc ctgcaccaac
     6601 gaagagtgta aaaacacacg taagatttta cgtaacggcg aagtggcacc accgaaagaa
     6661 gatccggtgc cattaccgga gctgccgtgc gaaaaatcag atgcctattt cgtgttgcgt
     6721 gacggtgctg ccggtgtgtt cctggcggcc aataccttcc cgaaatcgcg tgaaacgcgt
     6781 gcgccgctgg tggaggagct ttatcgcttc cgcgaccgtc tgccggaaaa actgcgttat
     6841 ctggccgatg cgccacagca ggatccggaa ggtaataaga ccatggttcg ctttagccgt
     6901 aaaaccaaac agcaatatgt ctcttcggaa aaagacggaa aggcgactgg ctggtcagca
     6961 ttttatgttg atggcaaa
//
LOCUS       DAZFFE010000169         6905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFFE010000169 DAZFFE010000000
VERSION     DAZFFE010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.170"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..495
                     /locus_tag="ACHJ69_004509"
     CDS             <1..495
                     /locus_tag="ACHJ69_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFO5017341.1"
                     /translation="LIKVLQSCQGKVVFIGVGKSGIIARKLAATFASTGTPSFFVHGT
                     EAVHGDLGMVAKDDVVILISNSGETAEILATLPSLKKMGNYLISFTRSHHSSLAISCD
                     LSVEIPVKSEADNLGLAPSCSSTVVLVVGDAVALALSELKKFTRADFGLYHPGGALGI
                     KANS"
     gene            567..1514
                     /locus_tag="ACHJ69_004510"
     CDS             567..1514
                     /locus_tag="ACHJ69_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO5017342.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDGYQADTLSLLPGVRIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGCELQGKRIGLVGYGNIGKTLA
                     KRLSGFDVELLAFDKQPDYQVADKAGVQFVSIEDIFMQSHVIIVLLPFSSGLENFISH
                     KYLSMMRNGALIINVARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGIHVAQSIIDYFAGREIKNVL"
     gene            complement(1814..3190)
                     /locus_tag="ACHJ69_004511"
     CDS             complement(1814..3190)
                     /locus_tag="ACHJ69_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO5017343.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     QVEKLCLALFRQNGVELQTLVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDL
                     LAGQFRNTALRLERLAPQGAETTITPPELPRREMNQPKKSEESPQPVFATRSVQQNDK
                     DASPPVPSPEISRQRTWPIFMAGMVVMAGLGGTGLWGWSQLNQPDALIQRIQLSVMPL
                     PLSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNAQWRKQRELSALSAEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEVSPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            complement(3190..3618)
                     /gene="tssE"
                     /locus_tag="ACHJ69_004512"
     CDS             complement(3190..3618)
                     /gene="tssE"
                     /locus_tag="ACHJ69_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO5017344.1"
                     /translation="MSTPSLYEMLTFSFSGELPLEQVSERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMAAGIHGLMRQIEETLLRYEPRLSQIQVELLPQPRPG
                     HLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            complement(3622..4158)
                     /gene="tssJ"
                     /locus_tag="ACHJ69_004513"
     CDS             complement(3622..4158)
                     /gene="tssJ"
                     /locus_tag="ACHJ69_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO5017345.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKN
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYYPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            complement(4139..5218)
                     /gene="tssG"
                     /locus_tag="ACHJ69_004514"
     CDS             complement(4139..5218)
                     /gene="tssG"
                     /locus_tag="ACHJ69_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO5017346.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQLLEQSQQNAPLGST
                     DSPATDAVRFRPHPGMGFPVSELKNVERDVGNPDAPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAVDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSPISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPGGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPAFSIRNRESMTHVSYSF"
     gene            complement(5182..>6905)
                     /gene="tssF"
                     /locus_tag="ACHJ69_004515"
     CDS             complement(5182..>6905)
                     /gene="tssF"
                     /locus_tag="ACHJ69_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO5017347.1"
                     /translation="RYLREAGKEFAQAYPDRAAMLNLDKPGARDPYVERLFEGFAFLM
                     GRLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELSTDHRQMKQSETLSDFQVLS
                     RPVGERRTRCVYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFACGPLVGDWSQIDL
                     SRLPLYLNADSPVACALHRALTLGTQQFWLRLPGQDRRMLGAHFSPMGFEDDDRLWPK
                     GESAFSGYQLLLEYFTFREKFMFVALNGLEQVAWPEGITGFEIDVVLNENWPHDLPFD
                     SDNIRLHCVPVINLFPLEADPLHLSPLENEFLLRPMRIQDGHTEIYSVDNIISSRHTG
                     SQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILGGDAFDTDRMLEDE
                     TLSLSLTGTNGQLPRKALQSTLLDTPVHASQNVLRVRNLCAPTQPCYPPARDRFHWRV
                     LSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLEAIVDVQHSLIQRFERGFLLR
                     GVDIQVTLDSNGFAGEGDITLFGELLHRFFALYADIHLFTQLTLILQPTGKCLQWTEH
                     HSQRVPG"
BASE COUNT         1607 a         1814 c         1836 g         1648 t
ORIGIN      
        1 ttaattaaag tattacaatc ttgtcaggga aaagttgttt ttattggtgt tggtaagtcc
       61 ggtattattg ccagaaaact cgcagcaact tttgccagta ccggaactcc ctcgtttttt
      121 gttcacggta cggaagcggt acacggcgac cttggaatgg tggcgaaaga cgatgttgtt
      181 attcttattt caaacagtgg tgagacggcg gaaatcctgg cgacactgcc tagtttgaaa
      241 aaaatgggta attatttgat ctcttttacc cgcagtcatc actcatcgct tgcgataagt
      301 tgtgatttat ctgttgaaat ccccgtcaaa agtgaggcgg ataatttagg tttagcaccg
      361 tcatgctcgt cgactgttgt tctggttgtt ggtgatgcgg tggcgcttgc actctctgaa
      421 ttgaaaaaat ttactcgtgc tgatttcggc ttatatcatc caggaggtgc actcggcatt
      481 aaagcaaatt cataatttca aaatataaat acagtaaccg gtcacgccat cttttggggt
      541 ggcgttcttt gaagtgagtg tccgctatga aaaatgttct ggtcaccgtc cccagctttt
      601 cagcacgctg tgtttccgcc agcaagttgc taagagagaa taattttaat cttattatta
      661 agaataatgt ggagcatctg cttaaatcag aatccactgc attacgagaa tctatttgcg
      721 cggtcatcgc cggaaaagat ggttatcagg cggacacgct atcattgctg cctggtgtga
      781 gaataatatc gagattcggg acggggatag acaatattga tcttcgtgcg gcacagcaat
      841 ccggtattgt tgtcaataat gcggttggga ttaattcaaa tgcggttgcg gaatttatca
      901 ttggacttat cttcgcgagc atgagaaata tccctggcag ctatcatgcg atgcaaaatg
      961 gctactgggg tgagtcgcat ggctgtgaat tacaagggaa acggattggc ctggttggct
     1021 acggtaatat aggtaaaact ctggcgaaaa ggttatccgg ttttgatgtt gagcttttag
     1081 cttttgacaa acaacccgac tatcaggttg ctgacaaagc tggagttcag tttgtatcaa
     1141 ttgaagatat ctttatgcag tcgcatgtca tcattgttct tttgcctttt tcttctgggc
     1201 tggagaactt cattagtcat aaatatttgt cgatgatgcg caatggtgct ctgatcatca
     1261 atgtcgcaag gggaaagttg ctggatgagg gcgctttact tcaggtgatt gaagaacgaa
     1321 atgtgtttgc ggcgctggat gtgtttagct ctgaaccgtt ggcgcaattt agcccactgc
     1381 tacatgccaa aaatatcatt acaacgccgc atatcgcggc cgccaccgtt gagtcatacc
     1441 agcaaaccgg gatacacgtt gcccagtcga taattgatta cttcgcagga agggagataa
     1501 aaaacgtgtt gtgagttcag cgaacaaaga ttttaaatac aaaaaaaccg caatttgtag
     1561 aacacattgc ggtttgtcag cattctaata aattggttgc gggggccaca gtcaagaggt
     1621 taatccaatc ttttgaaact taaggtattc actgttcccg aacccaagct atctaccgaa
     1681 atatacactc attatctgat gtgaatcaac gagtagatcg aatggcctgt tcctctcttt
     1741 ttttgtacac agagactcct ttaaaaaatg gcaaggtcac atatccctta ttggtacata
     1801 aatcccccga tgtttactga ctttgttcca ccttcgtatc cagtaaaatg acatagcgat
     1861 tcaacaactg atttaaccgg gtatcaattt gcgtcaacag cgttggcgaa acctctccgg
     1921 tctgtttttg ctcttccagt tgccgaagca gttcttccag cggcggctcc tttaacgact
     1981 gccggatgcc gtacactgcc gtttttagtt ccgaaccggt cagatatctc cccttacgtt
     2041 catccagtgc atccagctgc gccgacaggc gctgtagctg gctctgcgcc tgagcatagc
     2101 cattcagtgc ctcagcagac agggcgctca gctcccgctg tttgcgccac tgcgcgttca
     2161 gcgccctgac ctgaggatta tccggccaca gggcatcaag ctggcgtagc tggcgatatc
     2221 cctgctccag tggccagcgg gcaggcaatt tgtttaactg ctccagttgc atctgacttg
     2281 ccgcaattgt tctgtcctga gccagcagcg ccttatcctt tacatccagc tttgccagtt
     2341 cgccgctctc cagcgacagc ggcaacggca tgacagacag ttgtattcgc tggattagcg
     2401 cgtccggctg attaagctgc gaccagcccc ataatcctgt tccgccgaga ccggccatca
     2461 caaccattcc ggccataaat atcggccatg tccgctgccg ggagatttca ggggatggaa
     2521 cgggtggact ggcatcctta tcgttttgct gaacggatct ggttgcaaaa accggctgtg
     2581 gactttcctc tgactttttc ggttgattca tttcccggcg gggtaattca gggggagtga
     2641 tagtggtttc cgctccctga ggcgccagcc gttccagacg caatgcggta ttgcgaaact
     2701 gcccggccag tagatccagt ttcgtcatat gccacagttc acactgttgc agcgtggttt
     2761 cgatggcgga aagatgctgt acacaacggt agatctgcgg cagatcctga tactgaatat
     2821 ccagcgttct cagtgcctgc tgcatttttt ctgtgaccca gctgagcagt gatattctgg
     2881 aatgtacctg taccggccag aagtcagccc agcgctggag cagtatatcc agtgaaccga
     2941 gtccgtctgc catccctgcc agcccctgcc gtctggttat cgccagtacg taacagacca
     3001 gcgtctgtag ttccacgcca ttttgccgga acagcgcgag gcagagtttt tccacctgac
     3061 gccagtccac atccggtcgg gccgggtgag tcagtttact catttcagcc tgcagttcac
     3121 cggattcctt ataagtacag ggatcgccgc ccatgacgaa ctggctgata aaattcgcgt
     3181 tacttgccat cagtacgccc gctcctgttg tttgagatga cgcagaacaa ttcgtccttc
     3241 cggagaaaat acgccatcaa agcgtatcca gccggtatcg ggaagctgcg cgtggatcag
     3301 gtaattaaga tgccccggac ggggctgggg gagtaactcc acctgtatct gactcaggcg
     3361 tggttcataa cgcagcagcg tttcttcaat ctgacgcatc aggccatgaa tacccgcagc
     3421 catcccctga tggatcagac tgagatccgg cagaccgtaa tccggcaaat gcgccagcgt
     3481 gcctgcccga cagttgataa tgcgttgcat attgtccatc acggaaagga taagctgatc
     3541 ccgctcgctg acctgttcca gcggtaactc gccgctgaaa ctgaacgtca gcatttcata
     3601 taaggaaggt gtcgacatcg gttatttatc cttgagaggc agcagtcgca ggtcgcttct
     3661 catcagttca atcgagcgcg gcttgtcggc ctccagttcg tcccttttga tcaccagacg
     3721 ccagctgtcg cttttttcat cagggtaata aaactgccca ataatggcga caaactgcgt
     3781 ctctttatcc agcggcacat tcagggatgc cgtgccttcc ggttttaccc agacggtgtg
     3841 ttttgccagt acgttcttct ccagcgtctt ctcttcctgc ataaacaggg tgtcataatc
     3901 cgccttgtca aaatcttcgc gggttttcag ttgccagaca tgcaccatcg tggaaagcgg
     3961 cgtatcttcc gcatccgtat tcagggcact tcgggagaag aaatcgagat gcagtatttt
     4021 gatttgcttg tagaaaacgg ctgaggccac gcttttcgat tcatccacca ctttctgaat
     4081 cagcccgcag ccgctcagaa caaccgtcag catcgccata cagagcagcg ccggacgttt
     4141 aaaacgaata ggaaacatga gtcatactct ccctgttacg aatggaaaaa gcgggtatta
     4201 atccctgata acgccccaga ctgaccgtca ctgttccggt cgcgggcgcg gcaaaggagg
     4261 cgcgaaggat gccggtacgg ctaatctgta cacgctgttt tcccagccgg gcagcaggca
     4321 acaggctgac gggcagcgtc agttgcagtc gggcctgaca gcgccagccc agataaacgc
     4381 gcagcagcac cagcaaatcg ctgtgcagtt gcccgccggg cagccagcca cgtgcttctg
     4441 cggcatcttc ggtatacagt ttcagcagca actggctgtt gacgtcggtg ccggtacgcc
     4501 ccagcagcgt tcgcgttttc aggctgatgg gtgaattttt tgacagaccc gcaggggccg
     4561 ggagaattac aggctgcgga tcatgaggca ccaccgtcgc tttcgtcagc ggggcaagca
     4621 gtcccaccag tgccgtaacg ccttccgccg tgcgcgtcgg caggcgcatc acgctgagca
     4681 gggccagaaa acgggatacc ggcgtggcga tatggttctc gctgccggga atacccagcc
     4741 cgatgagtcc cagcaggcaa cgggaaatgt catccactgc acctgcttca aacgacgccg
     4801 ggtagttata tttgcgccag atgcggtaat actgggtgat aaagcgatgg ttaaaaatat
     4861 cgagaaacgc catcaccgcg tcatgtccgt catgacgctg ggtaatgtag tccagatagg
     4921 cggttggcag cggcgaatcc acgccgtaca ggccgagaaa agtggtgcgc accgtggggg
     4981 gcgcgtcggg attgcccacg tcacgttcaa cgttttttaa ctcgctgacc ggaaagccca
     5041 taccgggatg aggacgaaag cgcaccgcat cggtggcggg gctgtcggta ctgcccagcg
     5101 gcgcgttctg ctggctctgt tccagcaact ggcaaaaacg gtaaaaattg atccgcgcaa
     5161 tatccggggc cagccggtcg gtcagcccgg tacgcgctgg ctgtgatgct ctgtccattg
     5221 caggcacttt cctgtgggtt gcagaattaa ggtcagttga gtgaataagt ggatatcggc
     5281 atacagtgcg aagaagcggt gcagcagttc accaaatagc gtgatatcac cttctccggc
     5341 aaagccgtta ctgtccagcg tcacctgaat atccacgcca cgcagcaaaa agcctctttc
     5401 aaaacgctgt atcaggctgt gctgaacgtc gacaatcgcc tccagacgac ggcggttcat
     5461 ctcgctttcg gtccagtcgt acaatgccag cgttccgcgc agaatttcgg cgttatccat
     5521 catcgataaa aagttagaac caaggtgact gagcacccgc cagtggaagc ggtcacgcgc
     5581 cgggggatag cagggctgcg tcggagcgca caggttgcgc acccgcagga cgttctgtga
     5641 ggcatgaact ggcgtatcga gcagcgtgct ctgtaaagcc ttacggggaa gctgcccgtt
     5701 agtaccggtc aggcttaaag agagcgtttc atcctccagc atccggtcag tatcaaatgc
     5761 atcgccgccc agaatcagcc aggtgtcatg caggccggaa gggccgcgct ttacccgcgt
     5821 gtggtaatag cgttccggcg catcgtggcg cagcatcccg ccccggtgac gaaagctgga
     5881 gaaggggaca tacgcctgac tgccggtatg gcgcgaagaa ataatgttgt cgacggaata
     5941 aatctcggta tgcccgtcct gaatgcgcat cgggcgcagc agaaattcgt tttccagtgg
     6001 cgaaaggtgt aacggatcgg cttcgagcgg gaacaggttg atgaccggca cgcagtgcag
     6061 acggatatta tcgctgtcaa acggcaggtc gtgaggccag ttctcgttca gcaccacgtc
     6121 aatctcaaag ccggttatcc cctccggcca tgcaacctgc tccagcccgt tcagcgccac
     6181 gaacataaac ttctcgcgga aggtgaaata ctccagcagc aactggtaac cgctgaaggc
     6241 tgactcacct tttggccaga gcctgtcatc atcctcaaag cccatcgggg aaaagtgtgc
     6301 gccaagcatc cggcgatcct gccccggcag acgcagccag aactgctgcg tgccaagcgt
     6361 taaagcccgg tgcaacgcac aggctaccgg gctgtcggca ttgagataga gcggcaggcg
     6421 ggataaatcg atctgcgacc agtcaccaac cagcgggccg cacgcaaagc gcagacggat
     6481 aaccgagcgt ccgtccggtt catattgcaa tgacacgtcc ggcaacgcca gcggatgcag
     6541 cgtgatatca cgcgtggcac tgtagacgca gcgggttctg cgctcgccaa cagggcgcga
     6601 caatacctga aaatcgctca gcgtttcgct ctgcttcatc tgccggtgat ctgtcgacag
     6661 ttcaaccacg gaaagtgacg ggatggttcg caggtagtgg ggccacagca ggctgaccag
     6721 cccttcagtc agttccggca gatcgtcgtc cagcttttcg cgcaggcggc ccattaaaaa
     6781 agcgaagccc tcgaacaggc gctccacata gggatcgcgt gcgccgggtt tatcaagatt
     6841 aagcatcgca gcacggtcgg ggtaagcctg cgcaaattct ttacccgctt cacgcaggta
     6901 gcgca
//
LOCUS       DAZFFE010000170         6813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFFE010000170 DAZFFE010000000
VERSION     DAZFFE010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.171"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            51..461
                     /locus_tag="ACHJ69_004516"
     CDS             51..461
                     /locus_tag="ACHJ69_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO5017348.1"
                     /translation="MNQTIFLRSKQQQQFAINAILATTLDKDKPVTIRITDYKRNLAQ
                     NAKFHAMVADISRQVKWCGRWLKPEQWKVLLISGHAVATKQEADVLPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTSWAVDQGVRFTDRRYE"
     gene            458..649
                     /locus_tag="ACHJ69_004517"
     CDS             458..649
                     /locus_tag="ACHJ69_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO5017349.1"
                     /translation="MRRQRRSITDIVCENCNYLPTKRSRNKPRPVPKESDVKTFNYTA
                     HLWDIRWLRRRARFNCHSG"
     gene            673..963
                     /locus_tag="ACHJ69_004518"
     CDS             673..963
                     /locus_tag="ACHJ69_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO5017350.1"
                     /translation="MTNLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGTGTK
                     PDDLLAAWACSDCHNEIDRRTRILDNNDARLYHLEGVIRTLAILLKEGKIKS"
     gene            960..1322
                     /locus_tag="ACHJ69_004519"
     CDS             960..1322
                     /locus_tag="ACHJ69_004519"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5017351.1"
                     /translation="MNEYEFVLPWPPTVNTYWRRRGSQYYISDKGQKYRKDVQQIIRQ
                     LRLDIFTKSRLRITIIAEPPDSRRRDLDNILKGLLDSLIHAGFAEDDVQFDDIRVIRG
                     VKVLGGRVGIKITELENI"
     gene            1319..1519
                     /locus_tag="ACHJ69_004520"
     CDS             1319..1519
                     /locus_tag="ACHJ69_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFO5017352.1"
                     /translation="MNATIQTIPELLIQARGNMTEVSRKLNCNRATVRKYVGDKEGKR
                     HAVVNGVLMVHRGWDKGKATDA"
     gene            1512..1892
                     /locus_tag="ACHJ69_004521"
     CDS             1512..1892
                     /locus_tag="ACHJ69_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO5017353.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVSWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHELLVDCYVRGMTFMSLARKHECSDGTIGKRLQKA
                     EGVIEGMLMMLDVRLEMDRYVQRL"
     gene            2027..2620
                     /locus_tag="ACHJ69_004522"
     CDS             2027..2620
                     /locus_tag="ACHJ69_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017354.1"
                     /translation="MDIEDFLEAEDNDLSLWSDDILSIDWFVILKELLKYQNRLKLNY
                     SELLLLANFVSFHQDVDSQTLPSISLFATRMRTSRDAIQGVLVCLEEKDMLQKISYAQ
                     IDHDDDLRNIYDIKPLIRRLIWLLKSGCDKKHTCPLCGKVAISNEEIEKKFGFRICGN
                     KKRPQSWCRSCRSPKQRRLNTLLCGKKRARKSSPEVV"
     gene            complement(3589..3661)
                     /locus_tag="ACHJ69_004523"
                     /pseudo
     CDS             complement(3589..3661)
                     /locus_tag="ACHJ69_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3948..5921
                     /locus_tag="ACHJ69_004524"
     CDS             3948..5921
                     /locus_tag="ACHJ69_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5017355.1"
                     /translation="MAFKHYDVVRASSPSDLAGKLTQKLKEGWQPYGSPIISNAGYGA
                     EFIQAIVAEGDMTSPVVSPGGDGTRTVVVEPEYYFVIALAGQSNGMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACAYNDIIPADHCLHDVQDLSRFSHPKASAEQYGCVG
                     QGLHIAKKLLPFMPENAGILLVPCCRGGSAFTAGADGTFSESSGASDKSERWGVDKPL
                     YKDLLTRTQAALKANPKNILLCVVWMQGEFDLKQGAYASQPAMFDAMVEKYRSDLAGV
                     SGQCAGASPSLVPWICGDTTYYWKETYSAQYDAVYGAYKTKAGKNIFFVPFMMDDNGQ
                     KVGTNEPSEDPDIPAIGYYGSGGRTDAKSWTTSDRKTHFGSWARRGIISDRLATAILL
                     HVGRVADFVSGKVTKAAVSSGGGVSQPAQESGDTQPEAVVPTKIQSVLAYDVNAENAA
                     LASQGWTLTDVKNTLVSDRGSGKKALRLEKPENGLAQKKTWKVSHAIDAGKGKELFDN
                     GGEITLRFKIPDDVSLNASANQYSAGIYWRGSGWPGAESEEGYIAAFYLQTDKTNINV
                     MYHANTTSQRLGSYGPFDHDWHTLTFRFPGGGSLNVTPVLDNAAGKPFTLTRWTNAAF
                     EANALAITDITGNAATYPVLIESLTVGVNAVAA"
     gene            6063..6257
                     /locus_tag="ACHJ69_004525"
     CDS             6063..6257
                     /locus_tag="ACHJ69_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO5017356.1"
                     /translation="MTFINQLMLYFCTVVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SATQSAGAKTEAPLIPEQPS"
     gene            6283..6573
                     /locus_tag="ACHJ69_004526"
     CDS             6283..6573
                     /locus_tag="ACHJ69_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO5017357.1"
                     /translation="MTDIKQLVTAEAVKEVIRSEEVRSVLKQKLRQNLEERLDAEVDS
                     ILDELLGAQPEPSPELLPEPQAEDVTTENGDIQPESPVTDMTDTQPEPGTML"
     gene            6650..>6813
                     /locus_tag="ACHJ69_004527"
     CDS             6650..>6813
                     /locus_tag="ACHJ69_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5017358.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVTPSQWAAIGVLGS
                     LCLGFLTYLT"
BASE COUNT         1871 a         1526 c         1790 g         1626 t
ORIGIN      
        1 aaaggagctt cggctccttt tttgttggga aaatccagtg agagggaata atgaaccaga
       61 ctatcttcct ccgaagtaag cagcagcaac aattcgccat taacgccatc cttgcaacaa
      121 ctctcgataa agacaaaccc gttacgatcc gtatcaccga ttacaagcgt aatcttgctc
      181 aaaatgcaaa atttcacgcg atggtcgctg atatatcccg ccaggttaag tggtgcggca
      241 ggtggctaaa accagaacag tggaaagttt tgttaatcag cggtcatgcc gtggcgacaa
      301 aacaggaagc tgatgttttg ccaggtcttg aaggtgaata cgtcaatatc cgcgaaagca
      361 gtgcgcaaat gagcgtgaag cgtatggcaa gcctgattga gtacacaaca tcctgggccg
      421 tggatcaggg tgtcagattt accgacagga ggtacgaatg agacgacagc gacgaagcat
      481 caccgatatc gtctgtgaaa actgcaatta ccttccaacc aaacgctccc gaaataaacc
      541 caggccagtc cccaaagaat ctgatgttaa aaccttcaac tatacggctc acctgtggga
      601 tatccggtgg ctaaggcgtc gagcaagatt taattgtcac tccggatagc ctactgcatg
      661 aggaataaga tgatgacaaa cctacgcaaa gaagcgcgcg gcagagaatg ccaggtacgg
      721 atttatggcg tatgcaatgg caaccctgaa actacagttc tggcgcatta ccgaatggct
      781 ggaatttgcg gaacgggaac aaagccagat gatctgttgg ctgcatgggc ctgtagtgac
      841 tgccataatg aaatcgatcg ccgtactcgc attctcgaca acaacgacgc cagactttac
      901 cacctggaag gcgtgatcag gacgctggcg atattgctga aggaggggaa gattaagtca
      961 tgaatgaata tgagtttgtg cttccctggc cgccgacggt gaatacctac tggcgaagac
     1021 ggggaagcca gtactacatc agcgataaag gccagaaata ccgaaaagac gtacagcaaa
     1081 tcatccggca actcagatta gacattttca ctaaatcacg acttcgcatc acaattattg
     1141 ctgaaccacc agattcccgc cgtcgcgacc tcgataacat cctgaaaggt ttactcgact
     1201 ctcttatcca cgccggattt gcggaagacg acgtgcaatt cgatgacatt cgcgtaattc
     1261 gcggcgtgaa agtgcttggc ggtagagtgg ggataaaaat caccgaactg gagaacatat
     1321 gaacgccaca attcaaacaa taccggaatt gcttatccag gcacgaggca atatgactga
     1381 agtttcacga aaactgaact gtaatcgtgc tacggtcaga aaatatgtcg gtgataaaga
     1441 aggcaaacgg cacgccgtcg tcaacggtgt ccttatggtt caccgtggat gggataaggg
     1501 gaaagctact gatgcgtgat attcagcagg tgcttgaacg ctggggtgca tgggtggcaa
     1561 ataatcatga ggatgtgagc tggtcgccca ttgctgccgg atttaagggg ctgatccccg
     1621 aaaaagtaaa atcacgtcca cagtgctgtg atgatgacgc gatgattata tgcgggtgta
     1681 tggcccgcct taacagaaac aacagtgatt tgcatgagtt gctggttgat tgctacgtgc
     1741 gggggatgac gtttatgtca ctggcgcgaa aacatgaatg ctctgatggg actatcggaa
     1801 agcggctgca aaaagctgaa ggtgttattg aaggaatgct gatgatgctg gatgttcggc
     1861 tggagatgga cagatatgtg cagcgcctgt gatagcaggc gcttgtcgtt cactatgtga
     1921 agtatgtcaa tttgtgaacg ttatgctatt tactttacag gacaaggacg taagagttat
     1981 ctaccgttta accgcagaga tacaacaaca atgaacaggg ggcaaaatgg acattgaaga
     2041 ttttctggag gcggaagaca atgatctgtc tctgtggagt gatgatattt taagcattga
     2101 ctggttcgtt atcctcaaag agcttttgaa ataccagaac aggttaaagt taaactattc
     2161 agaattactt ctgctggcta attttgtttc atttcaccag gacgtagatt cgcaaacatt
     2221 accatcaata tctctttttg ccaccagaat gcgtacatcc cgggacgcca tacaaggagt
     2281 cctggtgtgt ctggaggaga aagatatgtt acagaagata agttacgcgc aaatcgatca
     2341 tgacgatgat ttgcgaaaca tctatgatat caagccatta atccgccggt taatttggtt
     2401 attgaaaagt ggttgtgata aaaagcatac ttgccccctc tgcgggaaag tcgctatatc
     2461 taatgaggaa attgagaaaa aattcggatt cagaatttgt gggaataaga aaaggccgca
     2521 aagctggtgt cgttcatgca gaagccccaa acaacgccga ctgaatacat tgttatgtgg
     2581 taaaaaaaga gctcgaaaat cctcgcccga ggtggtgtaa agcgtcgtag aaccccggtc
     2641 ctcttgtttg attccatacc tgaataaagt gggatgccaa aaaagtattt tacgtacgta
     2701 aaaatctgca tatcatgata agagtggtta cattgccacg cagccgaacc cgccgatgcg
     2761 cgggtttttt tgtacccaga atcctgtgag ctatacggaa agtacacaga aaggaaggtg
     2821 cgaccgcaat taataacaaa atcttaaaaa tatcatatgg cactattagt tttctaaata
     2881 ttgtatattt taagtattgc aggataaccc tgtaacgaag tttgcgtaac agcattttgc
     2941 tctacgagtt tgccagcctc ccctggtggc tggctttttt tgtatccgtt caacaggaat
     3001 gttacatacc tcacaattaa gtcagttgaa tgttgtctgc ccggatgaga atttgttaga
     3061 aaaaactgca tggtgaatcc ccctaagcgg cggggcatct ggcaaagtgt atgatccagc
     3121 gaacatgcaa attcagtagc caggctgaat ttaccgggag gcacctggca ccatgcgaca
     3181 gacagaaatt aggctatact tcagcccctc tccggagggg cttttctgtg caggatgtgt
     3241 cacagtttcc tgaattctga gtactgtcct gttactcagg gtgctatatt ttctgacgtg
     3301 atgaaagtct gccggaaggc ggaacgtatc ggaaatgacc cagtagagaa aacgttgact
     3361 cagataccga tgctgagtta ccgggaaacc ggcatcacat gaccgctatc cttccaggct
     3421 cgctccggcg ggccttttta ctgcagaaaa cagttttccc gtaaaatgcc acgttactca
     3481 caattcaggc tggcgattat tgtctggcca gcgggaagtt tgttaaaaaa atatggcatg
     3541 gtgaatcccc ctgtgcggag gggcatatca gcgacaggtg tttggtcaca ccccttatcc
     3601 tttctggtgc gggttcaggt gctgatactg aactcaccgg gaggcacccg gcaccatgca
     3661 tgaacggtac atagcgctac tctccagccc ctctccggag gggctttctt gttggataaa
     3721 aaaaagcccg cgccgggaag cgcgggtggc aaggaataaa caacaaaacg tgaagtgatc
     3781 aattttcagc tggcgaataa tacccgacag taatcactct gcgcaactgt atggtctttt
     3841 tcgtattgcg ggctgtagtc atcctcctgt ttctgaaccc ttgtccatga ctgacgtcag
     3901 cccgcacctt atcttgattg catacactat ccctgccggg aggaataatg gcatttaaac
     3961 actatgacgt ggtcagggca tcatcgccgt cagacctcgc aggaaaactg acgcaaaaac
     4021 tgaaggaagg ctggcaaccc tatggtagtc cgattatctc aaacgcgggt tatggtgctg
     4081 aatttatcca ggctatagtc gctgaagggg atatgacctc tccggtagtt tcaccggggg
     4141 gagatggtac ccggactgtt gttgttgaac cagaatatta ctttgtcatt gcgctggccg
     4201 gacagtcaaa cggcatggcg tatggtgaag gcctgccgct gccggagaca tatgaccgtc
     4261 cggacccgcg tattaaacag ctggcgcgtc gcagtacggt gacaccgggt ggtgcggcct
     4321 gtgcatataa cgacatcatt ccggcggacc actgcctgca tgatgtgcag gatttgagcc
     4381 gtttttctca tccgaaagcc agcgcagagc agtatgggtg tgtcggacag ggattgcata
     4441 ttgccaaaaa gctgttgccc tttatgccgg aaaatgcggg tattttgtta gtgccgtgct
     4501 gtcgtggtgg ctctgcgttt acagccggtg ctgacggaac gttcagtgaa agttcaggag
     4561 cgtcggataa atctgaacga tggggcgttg ataagccgtt gtataaagac ctgctgacac
     4621 gcactcaggc cgcgctgaag gcaaacccca aaaacatact gctttgtgtt gtctggatgc
     4681 agggcgagtt tgatttaaaa cagggagcgt acgccagtca gcccgccatg tttgatgcca
     4741 tggtggaaaa ataccgttct gacctggcgg gagtatccgg tcagtgcgct ggtgcttctc
     4801 cttctctggt gccctggatt tgtggtgaca cgacgtacta ctggaaggag acgtattcag
     4861 cgcaatacga tgccgtctat ggcgcataca aaaccaaagc cggtaaaaat atcttctttg
     4921 tgccgtttat gatggatgac aatggtcaga aagtcgggac caatgaaccg tcagaagacc
     4981 cggatattcc ggctatcggg tattatggtt ccggtggacg aacggatgcg aagagctgga
     5041 caacatccga tcgtaaaacc cactttggtt catgggcgcg tcgcggaatt atttccgacc
     5101 gtctggcaac agccattctt ctgcatgtgg gcagagtggc agattttgtt tccgggaaag
     5161 tgacaaaagc cgcggtttct tcaggcggag gcgtatcgca accagcacag gagagtggcg
     5221 atactcagcc tgaagccgtt gtcccgacaa agatacagtc agtgctggct tatgatgtga
     5281 atgcggaaaa tgccgctctg gcttcgcagg gctggacgct gacagatgtc aaaaacacgc
     5341 tggtcagtga ccgtggttca ggtaaaaaag cccttcgtct tgaaaaacca gaaaatggtc
     5401 ttgcacagaa aaagacctgg aaagtatcgc acgcaattga tgccggtaag gggaaggagc
     5461 tgttcgataa cggcggtgaa atcacgctgc gctttaaaat ccccgatgat gtatctctca
     5521 atgcttctgc aaatcaatat tctgcgggta tttactggcg cggtagtgga tggccgggag
     5581 cggaatcaga ggagggttat attgcggcct tctatctgca gactgataaa acaaacatca
     5641 atgtgatgta tcatgcaaac accacatcac agcggcttgg tagttatggt ccgttcgatc
     5701 acgactggca cacgctgact ttccgcttcc cgggtggtgg ctcgctgaac gtcacgccgg
     5761 tgcttgataa tgcagcagga aaaccgttta cgctgaccag atggacgaat gcagcctttg
     5821 aggccaacgc gctggcaatt acggatatta ccggaaatgc ggcaacttac cctgtgctga
     5881 ttgaaagtct tactgttggt gtgaatgccg tagcagcata acagacgaaa aaaaaccgcc
     5941 agcaacagga atggagactg gcggaggtaa tcccaatgga gaatctaacg aaaggatgct
     6001 ttcgacatca atcatttcta aatgaaaaca gttctcattg tcaacagtaa cggtaataaa
     6061 ccatgacatt catcaaccag ttaatgctgt acttctgtac ggtggtctgt gtgttgtatc
     6121 ttctttcagg tggatacagg gcagtacgcg acttctggcg cagacagatt gacaaaaggg
     6181 ccgctgagaa aatcagcgcc actcagtcag ccggagcaaa aacagaagcc ccactcattc
     6241 cggaacaacc ttcttaataa cccctttcaa cgagaaaatc tcatgacaga tataaaacaa
     6301 ctggtcactg ctgaggcagt gaaggaagtc attcgctctg aagaagtcag aagcgtcctg
     6361 aaacaaaaac tccgccagaa tctggaagag cgtcttgatg cagaagtgga ttcaattctg
     6421 gatgaattgc ttggtgcaca gccggaacca tccccggaac tgcttccgga accacaggcg
     6481 gaagatgtca ccacggaaaa tggtgatatt cagccggagt caccggtgac ggatatgaca
     6541 gacacacaac ccgaaccggg cacaatgctg taacggcggg gcagggccat cagtaagaca
     6601 ctgatggcct tttttatttc cggtagcaca ggtctgtcgg ggcggggata tgtatcagat
     6661 ggaaaaaata tcaacgggca tagcctatgg tacttctgcg ggcagtgcgg gttactggtt
     6721 tctacagtgg ttggatcagg ttactccgtc acagtgggct gctatcgggg ttttggggag
     6781 cctgtgtctg gggttcctga catacctgac gaa
//
LOCUS       DAZFFE010000171         6803 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZFFE010000171 DAZFFE010000000
VERSION     DAZFFE010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.172"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            296..409
                     /gene="yhiY"
                     /locus_tag="ACHJ69_004528"
     CDS             296..409
                     /gene="yhiY"
                     /locus_tag="ACHJ69_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO5017359.1"
                     /translation="MMTTLLPVFTKPSPLALNALCAGRICHFLLIPDGRIR"
     gene            472..1539
                     /gene="yhiI"
                     /locus_tag="ACHJ69_004529"
     CDS             472..1539
                     /gene="yhiI"
                     /locus_tag="ACHJ69_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO5017360.1"
                     /translation="MDKSKRHLAWWGVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1536..4271
                     /gene="rbbA"
                     /locus_tag="ACHJ69_004530"
     CDS             1536..4271
                     /gene="rbbA"
                     /locus_tag="ACHJ69_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO5017361.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPPELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDISRGTPVKLGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4271..5395
                     /gene="yhhJ"
                     /locus_tag="ACHJ69_004531"
     CDS             4271..5395
                     /gene="yhhJ"
                     /locus_tag="ACHJ69_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5017362.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            5468..5734
                     /locus_tag="ACHJ69_004532"
     CDS             5468..5734
                     /locus_tag="ACHJ69_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO5017363.1"
                     /translation="MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLRKP"
     gene            5731..6090
                     /locus_tag="ACHJ69_004533"
     CDS             5731..6090
                     /locus_tag="ACHJ69_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO5017364.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6140..>6803)
                     /locus_tag="ACHJ69_004534"
     CDS             complement(6140..>6803)
                     /locus_tag="ACHJ69_004534"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HFO5017365.1"
                     /translation="KRHADKMDVPVSLHLDHGKTLEDVKQAVRAGFTSVMIDGAAFSF
                     EENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKTFVERTGCD
                     MLAVSIGNVHGLDDIPRIDIPLLKRIAEVSPVPLVIHGGSGIAPEILRSFVNYRVAKV
                     NIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMNEGHRLIRK
                     AS"
BASE COUNT         1548 a         1721 c         1903 g         1631 t
ORIGIN      
        1 aacctttatc atctatcatc ttttcagaat aaattatttt atattcggta tttattcgca
       61 catcaggcga taataacgct atgtgatagt catcaggttt ccctgttacg gaaacctgtg
      121 caacagaatc tgtgaacctg tacagaatat ttttggaaaa aacaccagcg aacggaagaa
      181 catcgcgatg tatcacaagt tgatggcaat caaatagaca acatgaatat aactgttagt
      241 atttaattga tttcacttat gagtatgcgt gtttcattta cggccaggaa tgctgatgat
      301 gacaacatta ctacccgttt tcactaagcc ttccccattg gcgctcaatg ctctgtgcgc
      361 tggtcgtatt tgccatttcc ttcttatccc ggatggaaga atccgctgaa catcgttcgg
      421 tggcagttgt aacacgcgtc gattgcttac agggattatg gcggatcgat tatggataag
      481 agtaagcgcc atctggcgtg gtggggtgtc ggggcactgg cggtggctgc cgtcgtggcg
      541 tggtggctgt tgcgcccggc gggtgtgccg gaaggctttg ctgtcagcaa tgggcgcatt
      601 gaagcgacgg aagtggatat cgccagcaaa attgccgggc gtatcgacac cattctggtg
      661 aaagaaggcc agtttgttcg agaaggtgaa gtgctggcga agatggatac tcgcgtgttg
      721 caggaacagc gactggaagc catcgcgcaa atcaaagaag cacaaagtgc cgttgccgcc
      781 gcgcaggctt tgctggagca acgccaaagc gaaactcgtg ccgcacagtc gctggttaat
      841 caacgccagg cagaactgga ctccgtagca aaacgtcata cgcgttcccg ctcactggca
      901 caacgggggg caatttctgc gcaacagttg gatgacgacc gcgccgccgc tgagagcgcc
      961 cgagctgcgc tggaatcggc aaaagctcag gtttctgctt ctaaagccgc tatagaggcg
     1021 gcacgcacca atatcattca ggcgcaaact cgcgtcgaag cggcacaagc cactgaacgg
     1081 cgcattgccg cagatatcga tgacagcgaa ctgaaagccc cgcgtgacgg acgcgtgcag
     1141 tatcgggttg ccgagccagg cgaagtgctg gcggcaggcg gtcgggtgct gaatatggtc
     1201 gatctcagcg acgtctatat gaccttcttt ctgccaaccg aacaggcggg cacgctgaaa
     1261 ctgggcggtg aagcacgttt gattcttgat gctgcaccag atctgcgtat tcccgcgacc
     1321 atcagttttg tcgccagcgt cgcccagttc acgccaaaaa ccgtcgaaac cagcgatgag
     1381 cggctgaaat tgatgttccg tgtcaaagcg cgtatcccac cggaattact ccagcagcat
     1441 ctggaatatg tcaaaaccgg attgccgggc gtagcgtggg tgcgggtgaa tgaagaactt
     1501 ccgtggcctg acgacctggt ggtgaggttg ccgcaatgac gcatctggaa ctggttcccg
     1561 tcccgcctgt cgcgcaactg gcgggcgtga gccagcatta tggaaaaacc gttgcgctga
     1621 acaatatcac tctcgatatt ccggcccgct gcatggttgg gctgattggt ccggatggcg
     1681 tcgggaagtc gagtttattg tcattgattt ccggtgctcg tgtcattgag cagggcaatg
     1741 tgatggtgct gggcggcgat atgcgcgatc cgaagcatcg ccgtgacgtc tgcccgcgca
     1801 tcgcctggat gccgcagggg ctgggtaaaa acctctacca caccttgtcg gtgtatgaaa
     1861 acgtcgattt tttcgcccgc ctgttcggtc acgacaaagc ggagcgggaa gtgcgtatta
     1921 atgagctgct gaccagcacc ggattagcac cgtttcgcga tcgtccggca gggaaactct
     1981 ccggcgggat gaagcaaaaa cttgggctgt gctgtgcgtt aatccacgac ccggaattgt
     2041 tgattcttga tgagccaaca acgggggttg acccgctctc tcgcgcccag ttctgggatt
     2101 tgatcgacag tattcgccag cggcagagca atatgagcgt gctggttgcc accgcctata
     2161 tggaagaggc ggaacgcttc gactggctgg tagcgatgaa tgccggagaa gtgctggcaa
     2221 ccggtagcgc cgaagaactg cggcagcaaa cgcaaagtgc cacgctggaa gaagcattta
     2281 taaatctgtt accgcaagca caacggcagg cgcatcaggc ggtagtgatc ccaccgtatc
     2341 aacctgaaaa cgcagagatt gccatcgaag cgcgcgatct gaccatgcgt tttggttcct
     2401 tcgttgccgt tgatcacgtc aatttccgca ttccacgcgg ggagattttt ggttttcttg
     2461 gttcgaacgg ctgtggtaaa tccaccacca tgaaaatgct caccggactg ctgcccgcca
     2521 gcgaaggtga ggcgtggctg ttcgggcaac cggttgatcc aaaagatatc gatactcgtc
     2581 gccgggtggg ctatatgtcg caggcgtttt cgctctataa cgaactcacc gtgcggcaaa
     2641 acctggagtt acatgctcgt ctgtttcata tcccggaagc ggaaattccc gcgcgagtgg
     2701 ctgaaatgag cgagcgtttt aagctcaacg acgtcgaaga tgtattgccg gagtcattgc
     2761 cgctcggcat tcgccagcgg ctatcgctgg cggtggcggt gattcatcgc ccggagatgc
     2821 taatcctcga tgagccgact tctggcgtcg atccggtggc gagggatatg ttctggcagt
     2881 tgatggtgga tctctcgcgt caggacaaag tgactatctt catctccacc cactttatga
     2941 acgaagcgga acgttgtgac cgtatctcac tgatgcacgc tggaaaagtg ctcgccagcg
     3001 gtacaccgcc ggaactggtt gagaaacgcg gagccgccag tctggaagag gcatttatcg
     3061 cttatttgca ggaagcggca gggcagagca acgaagccga agcgccgcct gtggtacatg
     3121 acactaccca cgcgccacgt cagggattca gcctgcgccg tctgtttagc tacagccgcc
     3181 gcgaagcgct ggaactgcga cgcgatccgg tacgttcgac gctggcgctg atgggaacgg
     3241 tgatcctgat gctgataatg ggttatggca tcagtatgga tgtggaaaac ctgcgctttg
     3301 cggtgcttga ccgcgatcaa accgtcagta gccaggcgtg gacgctcaat ctctccggtt
     3361 cccgttactt tatcgaacag ccgccgctca ccagttatga cgagcttgat cgtcggatgc
     3421 gtgcgggcga tatcacggtg gcaattgaga tccctcctaa tttcgggcgc gatatctcgc
     3481 gtggtacgcc cgtgaaactc ggtgtctgga tcgacggtgc gatgccgagc cgcgccgaaa
     3541 cggtaaaagg ctacgtgcag gccatgcacc agagctggct acaggatgtg gcgagccgac
     3601 agtccacacc cgccagccaa agcgggctga tgaatattga gacgcgctat cgctataacc
     3661 cggacgtaaa aagcctgccg gcgattgttc cggcggtgat cccgcttctg ctgatgatga
     3721 tcccgtcaat gctaagcgcc cttagcgtgg tgcgggaaaa agagctaggg tcgattatca
     3781 acctttacgt gacccccacc acccgtagcg aatttttact tggcaaacag ctgccataca
     3841 tcgcgctggg gatgctgaac tttttcctgc tctgcgccct gtcggtgttt gtgtttggcg
     3901 taccgcataa aggcagtttc ctgactctca ccctggcggc gctgctgtat atcatcatcg
     3961 ccaccggaat ggggctgctg atctccacct ttatgaaaag ccagattgcc gccattttcg
     4021 gtacggcaat tatcacgttg atcccggcga cacagttttc cgggatgatc gatccggtag
     4081 cttcgctgga aggtccagga cgttggatcg gcgaggttta cccgaccagt cattttctga
     4141 ctatcgcccg cggaacgttc tcgaaagcgc tggatctgac tgatttgtgg caacttttta
     4201 tcccgttgct gatagccata ccgctggtga tgggcttaag catcctgctg ctgaaaaaac
     4261 aggagggatg atgcgccatt tacgtaatat ttttaatctg ggtatcaaag agttgcgcag
     4321 tctgctcggt gataaagcga tgctgacgct gattgtcttc tcgtttacgg tgtcggtgta
     4381 ttcgtcagcg accgttacgc caggatcgtt gaacctcgcg ccgatcgcca ttgccgatat
     4441 ggatcaatcg cagttatcga accggatcgt taacagcttc tatcgcccgt ggtttttgcc
     4501 accggagatg atcaccgccg atgagatgga tgccgggctg gacgccggac gttatacctt
     4561 cgcgataaat attccgccta attttcagcg tgatgtcctc gccggacgcc agccggatat
     4621 tcaggtgaac gttgatgcca cgcgcatgag ccaggcattt accggcaatg ggtatatcca
     4681 gaatattatc aacggtgaag tgaacagctt tgtcgcgcgc taccgtgata acagcgaacc
     4741 gttggtatcg ctggaaaccc ggatgcgctt taacccgaac cttgatcccg cgtggtttgg
     4801 cggggtgatg gcgatcatca acaacattac catgctggcg attgtattga ccggatcggc
     4861 gctgatccgc gagcgtgaac acggcacggt ggaacactta ctggtgatgc cgataacgcc
     4921 gtttgagatc atgatggcaa aggtctggtc gatggggctg gtggtgctgg tggtgtcggg
     4981 attatcgctg gtgctgatgg tgaaaggcgt gctgggcgta ccgatagaag gctcgatccc
     5041 gctgtttatg ctgggcgtgg cgctcagtct gtttgccacc acgtcaatcg gcatttttat
     5101 ggggacgata gcgcgttcaa tgccgcaact ggggctgctg gtgattctgg tgctgctgcc
     5161 gctgcaaatg ctttccggtg gttccacgcc gcgcgaaagt atgccgcaga tggtgcagga
     5221 cattatgctg accatgccga cgacacactt tgttagcctc gcgcaggcca tcctctaccg
     5281 tggtgccgga ttcgaaatcg tctggccgca gttcctgacg ctgatggcaa ttggcggcgc
     5341 gtttttcacc attgcgctgc tgcgattcag gaagacgatt gggacaatgg cgtaaggaag
     5401 ggaaacttga cttcatattg tgatgtcata ctattgatgt cataaagtga agtcataagg
     5461 acatgccatg agaacccagg tacaggcttt acggaagaaa caaaaaaata cattggatca
     5521 aatatttaaa tctcctgttc ctcaagggat taagtggtct gatatagagt cactggttaa
     5581 agcattaggc ggagaaatta aggaaggaag aggttcgcgt tgtaagttca tactaaatat
     5641 gagcgttgcg tgtttccatc ggcctcatcc gtcgccagat accgataaag gcgctgtaga
     5701 aagcgtgcgt gactggttac taagaaaacc atgatcaaac tgaagacgcc aaactctatg
     5761 gagattgccg ggcaacctgc tgttatcact tacgtgccag aactgaatgc atttcgtggc
     5821 aaattcctgg gcttgtcagg ttattgtgat tttgtctctg acagcattca ggggctgcaa
     5881 aaagaagggg agctttccct gcgagagtat cttgaggatt gtaaagctgc gggtattgag
     5941 ccgtatgcgc gtacagaaaa gattaagacc tttacattac gctatccaga atcgctgagt
     6001 gaacgcctta ataatgcggc tgcgcagcaa caagtctctg taaatactta tattattgaa
     6061 acgctaaatg agcgtttgaa tcatttataa cttatagcgg cccaacaatt tctgttgcgc
     6121 cgctatattt ttgtggctct taagatgcct ttctaatcaa tcgatgcccc tcattcatca
     6181 tcaggatttt gctatacaca tcttcctcga cggcattttt tgctgatgcc atcacccgtg
     6241 ccagattcgc ctcgttgtga ttattcacat acgctttgcc gactgcggtg ataaacgctt
     6301 tgcgcagatc gctggcgata ttcactttag cgacgcgata attaacgaaa ctgcgtaata
     6361 tctccggggc gataccggag ccgccgtgaa taaccagcgg caccgggctg acttcggcaa
     6421 tgcgcttcag cagggggata tcgatccgcg gtatatcgtc caggccatga acgttaccaa
     6481 tagaaactgc cagcatgtcg caaccggtgc gttcgacaaa ggtcttcacc ttttccggtt
     6541 ccgttttgca atcggcttcg ctgacgtgat catcttcttt gccgagaatc gcccccagct
     6601 ccgcttcaac cggcacgcca taggatttac agaaatcgac agcttcctgg gtgaaagcga
     6661 tattctcttc aaaagagaat gcggcaccgt caatcatgac cgaggtaaag cccgcgcgca
     6721 ccgcctgttt aacatcctcc agggttttcc cgtgatccag atgaagactg accggaacat
     6781 ccattttatc ggcgtgacgt ttc
//
LOCUS       DAZFFE010000172         6779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFFE010000172 DAZFFE010000000
VERSION     DAZFFE010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.173"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..558
                     /locus_tag="ACHJ69_004535"
     CDS             <1..558
                     /locus_tag="ACHJ69_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFO5017366.1"
                     /translation="IRKIKSGFSKYLLNNSFNKFSLGYNVISGSIHPTKFTNADGEPV
                     NEVLSSDLGILNDNTKPPLDSYLFEEVFYDSELERLNITDGEIQSVAVFTKIPKNSIK
                     IPVAGGYTYSPDFAYIVKTIKGDYLNFIIETKNVDGKDSLRLEEERKIKHAQALFSQI
                     SKSVKVEFRTQFSTDRIAEMIKQVG"
     gene            complement(1115..1513)
                     /locus_tag="ACHJ69_004536"
                     /pseudo
     CDS             complement(1115..1513)
                     /locus_tag="ACHJ69_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
     gene            1648..1752
                     /locus_tag="ACHJ69_004537"
                     /pseudo
     CDS             1648..1752
                     /locus_tag="ACHJ69_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(1760..2740)
                     /gene="nanS"
                     /locus_tag="ACHJ69_004538"
     CDS             complement(1760..2740)
                     /gene="nanS"
                     /locus_tag="ACHJ69_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO5017367.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDKEDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSEPHGASHDACRWGSDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVDAFRRALKQYHSQLNKIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNVPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISNRFVEAILQFWRER"
     gene            complement(2805..3911)
                     /gene="nanM"
                     /locus_tag="ACHJ69_004539"
     CDS             complement(2805..3911)
                     /gene="nanM"
                     /locus_tag="ACHJ69_004539"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO5017368.1"
                     /translation="MNKTITALTIIMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTTIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVASPDGVAGGFAGMSNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSADIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            complement(3931..4647)
                     /gene="nanC"
                     /locus_tag="ACHJ69_004540"
     CDS             complement(3931..4647)
                     /gene="nanC"
                     /locus_tag="ACHJ69_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO5017369.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6102..6704
                     /gene="fimB"
                     /locus_tag="ACHJ69_004541"
     CDS             6102..6704
                     /gene="fimB"
                     /locus_tag="ACHJ69_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO5017370.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEIQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIVSTSGGNAGLSLEIHPHMLRYSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT         1929 a         1420 c         1379 g         2051 t
ORIGIN      
        1 atcagaaaga ttaaatctgg ttttagcaag tatttgttga acaattcgtt taataaattc
       61 agtcttggat ataatgtgat ctcaggttca attcatccta caaagttcac taatgcagac
      121 ggtgagcctg tgaatgaggt attatcaagt gatcttggca tattgaatga caacacaaaa
      181 ccacccttgg attcttatct ttttgaggaa gtcttttatg actcagagct tgaacggctc
      241 aacataacag atggagagat ccagtctgta gctgttttta caaagatacc gaagaactcc
      301 attaagatcc cggtggcagg tggatacacg tactcccctg actttgcata tattgtcaaa
      361 acaataaaag gtgattattt aaactttatt atcgaaacaa aaaatgttga tggaaaggat
      421 agcttaagac ttgaagaaga acgtaagata aaacatgcgc aagcattatt tagtcaaata
      481 agtaaatctg ttaaagtcga gttcagaaca caattttcta cagacagaat agctgaaatg
      541 attaaacaag ttggatgata atatgttgta aatggaaaag ggtcttacgg cccttttgtt
      601 ttcctttaaa gggaactttc cattagatag aatacatatc cccacacttg gggtaaatca
      661 atcagctcat tgggctgtta actacttctc atgtatgggg caatcagacc ataatcataa
      721 ctaccgacca agaataaatt caaacttcca caataaaatg tggagggtaa aataagttcc
      781 tgacgccttg tctcttttat atcctatgtg atatttctgt aaatcaggaa aaagtattta
      841 ataacttttg ttattacggt gaattaccaa ttaacttgta ccgccacaaa aaatcaatcc
      901 ttattcgttt ttacgagtat attattcctg tcttcctacc atattctcat gaaatttttt
      961 cctctcagtt ataactttac tttatcttca gcactaagat tcaaagattg taatactgat
     1021 ctttcccagc aataatggac acgtggctaa gtgtgtaaac tctcagtcag aagtaactca
     1081 catgacatag tatcaaccag ataggtgtat tacctcaccc cctaaaaaac tctttccgat
     1141 gccactctac aataccctca aaagggtaat ttttacgcac ctgtggcaac gcaataattt
     1201 ttccgtcaaa atcccagaat gacctctcaa tgatctctcc gccgtttacc gcatccgata
     1261 ccagtacccg cagattttca tccaggccaa tcgtttcttt atcaaaggct ttatgatgta
     1321 tcgcgcaaag cgccagacca ttgggaattt cacaagggcc atcacgctgt ttccatttta
     1381 tatgtgtcgc ttccagtgag acggtggagt tgtcgtgacg aatattaaag ctacatattg
     1441 cgcactggta gttataagcg cgtaatacat gtttgcgaaa taatgggtca cgctgcttac
     1501 ggatttgctg gagagcaaaa aaagctcgtc agcgatttac tggaagccat tgatatccta
     1561 gcagtgaaat aattgcgatc atgctcgcac attttctact gttcaagcct gcaagcgaag
     1621 atatattacc ttgaactcat ttgttttttg ctgcctgtgt gccctaattg ccatgcgatg
     1681 ttgcaccgac gcaaaccacc gtttacgccg gaggagttaa aagcgctgat ggatgcgaat
     1741 aaatcaaatt aatgaacgct cacctttcgc gccaaaactg caaaattgct tctacaaacc
     1801 tgttagaaat aatccctcga cgggctgccg tgctgaaatg actgcttcgc agtgccgtcg
     1861 tccagttctc cggtgaccgg tacgctgaac cgtaatatcc cgtgcttaaa tcgtccgggt
     1921 cttcatcagg cacgttcgtc agtcctcttt caccttgttg ctggaagtcg acgaaaataa
     1981 tattggctaa aacattattt tgataattgc cataaatagc ttcatacgca tgagggaaat
     2041 tttctttcca gtaccaggtg gtatcgccgc aaaaccacgg cgcgtcagta attttattaa
     2101 gctgagaatg gtattgtttt agagccctac gaaaggcgtc aaccatatga ttaaagtgtt
     2161 gagggtgtga cgcgtagtca ctggtcatta agtcaaattc gccttgcatc cagcatacgc
     2221 cgaggaattt gttctgcgga ttttttgcca gtgcggctcg cgttctgctg actaaatcct
     2281 ggtatagcgg agtatccgat ccccaacgac aagcatcatg gctggctccg tgcggttctg
     2341 aatatgtccc ttcgctgccc gcggtaaaag ctgatccgcc acggcaacac ggaacgatga
     2401 gaacccctgc attatcagga ataaagggca gtaatttccg tgctatatgc agtgcctggc
     2461 caacggtgcc gtactgtgtt tgatgattcg ttgccagagg atggtgataa ccctgcatat
     2521 cctgaacatc gtgtgggcag tgagtcagtg gaataatgtc gttaaagtga catgacgggc
     2581 ctccgggatg tgtatgcgca aatctcgcta attgtttaat tctgggatga ggcgcatctt
     2641 ccttgtccgg taatggtagt ccttcgccat acgccatggc attagactga ccagcaacgg
     2701 taagaacata gtaataatcg ggcgatatta ttgcgttcat ataattttcg cgtaatacaa
     2761 atgacaaatt gatagcaggg cccctttacc ggggccctga agcgttagtt ttgtactgtg
     2821 actttattat ccttcacaga aatcaaaact gaatccgtca ccgctttgcc gccagcagtt
     2881 tcaccgccaa taattaataa actattattc cagggcaatg atactccgta ggcccgacct
     2941 tgcgataatt cgcccgattt atcccatttc ccgttatgcc aaagatgaat atcagcgcta
     3001 tatgattttt tcaggccttc atgcgcatag ttcttaccgt tctggtaatt ttctcgtgaa
     3061 cctttgaatc cggcccctcc ggcaaatata agtgagtcat tgctcatgcc tgcaaaaccg
     3121 ccagcgacgc catctggtga tgcgacggga gcaagcttat tccattttaa attattaccg
     3181 gtgaaatcaa gttcaaatac ggcatccgtt cgcaatcctg gtttggcttc gccattaata
     3241 agccaggttt tatcaccttt attcacaacc gccgcaccag ccgttccgta ccagggagat
     3301 tcgccagcgt aactccattg ctgtgtagag ggatcaaaag acaacagaaa cttattgaag
     3361 aaataatctt ctgctttttt gtcaaaatag tgggcgttga ttttatctat agtggttgaa
     3421 tcttttccag cttcgttgag atcttcaaaa tagccattga agatattctg gttaacaccg
     3481 ccagtaacat aagccttgcc gttgtgtaca aaagtcacat ggcccgccat gcccatcggc
     3541 gcgtgcgaca tcaatttaac ccagctattg gttttggggt tgtatttgtg tacgtcatta
     3601 aatacctgag tcaagccctc gctgtttttg ccaatgccgc caaacacata cagattgcca
     3661 tcaataaatg cagaggttgc ttgatctctc ggtccgccag ggaatgcagc taacgctgtc
     3721 cattttttat ctttggcctg tgtatccagc ttgtaccatg ccgtacctgc gctacctaaa
     3781 ccaatgtaga cagtgtcgtt atcaattgct ccggtaccac ttttaaatgg cacaggagtt
     3841 tccggtaata cagacgcgtt tgcggcaaat gaagccatta tgatagtaag cgccgttatt
     3901 gttttattca ttgtgactgt ctcctgtcta ctacagttta aatgacacac caatgcgata
     3961 actgttttcc gataaattat ctctgccgtt gtaaacaccc tgacggtcaa ggtagtcata
     4021 ttctatgtat ggcgtaatat cgggcgtcat atggtattgt agaacaaatg cattttccgt
     4081 cgcccatttc ttatggtttg catagcgata atcgttctgt ttgctgtata gcgtcgtttg
     4141 ccatgcgaag gtgaaatcac tattaatatg gtaagtgaca tatccatccc aacgatgaac
     4201 gttatcacga gacatatcac cggataagtc ttgttgtcgg taagctttcc agtcgtaacg
     4261 atagcgaatg ccaaaattaa gatcttttgt cgcgtcccag gacagtttta cgtagggtcc
     4321 gtatcgtgtg ccgttgctgc taaaatgcgt taacattccc gggcgcaccg tccattgatc
     4381 atcaagttta atcgcgtaat taacttccac ctgaacatca ttgagtgcgg cattttcctt
     4441 tttattatca tgaatggtat tccaggtatt actttccata cttgcccacc atccattttg
     4501 ccatccctca ctgactttga gtcgagtctc ataggcgtgg cttccactac gatatccacc
     4561 acgtacgtcc agtgtcgcag cctgggaaat tagtggtgac gagaagcaca gtaataatac
     4621 gccagaaagt attttagcct ttttcatata tttcactcat ttgtaggata cagaaagcaa
     4681 tacaaagccc gcataaacaa ttagcattta tgttgtgtaa tattttttgc caggcttata
     4741 gtgtctttgg caacgagtat ctgtatttta tattttttgt ataaggtctc ctgtgaaaat
     4801 tctctttcca cattattcaa ataaacagag aaccagatta aatacctgag tataaaatct
     4861 cttctgatgt ttaattgatt tgaatgttcg taagctatat cacttactca atccatttta
     4921 cccagagtca taaaatagag tgtctgttga tctcattttc atattccttt cagactgaag
     4981 tgcgttttca tcacccgatg agaaagaact gaagtgatgt ttgccataga ttgcgagatg
     5041 ccctgtctca ttgactatcg cggtgcacct ttatacctgt tataccagat caaaaatcac
     5101 gcaatccata caacaaaacg agatttgcaa ttcgtgtcac aaaatatgtc gatctttttc
     5161 taagaggaag atgccatgtg aagccagacg aacacttgag gtggtcttca aaaactaaag
     5221 gatttctttt aactgtttgt tttataaaca atttatttat ggtctaaaca agggatgctt
     5281 tgcaagctaa ctcagtgagc gtggtgaaaa taagtgttta cccgccatca ggatgatcat
     5341 aattctcatc atgaaatatg tttcctggtt tttggcttgt aagtggtcac ccctgaagtc
     5401 gatctggaaa ggcttgtgga tgttggtgtt ttcaggatga tgtttcagat aggttgtttg
     5461 ccgtatcgcc cggcgaatag ctgtgattga ggaaggttta agtcgcagtg accaaagcca
     5521 tatttaccaa cgaatgtaga tgaaaaagcg tctcctgcgt tcccccatat ctccaggaaa
     5581 tacacagtct gaaaacaatc tcatggcgta agctgacgaa ccaggcagga ataatcgcta
     5641 gggacctaag cattagcatg ataatagcca ctaagaaatt actgcgttcc atgaaatagc
     5701 cattttgtgg caaatgcagt tgactaataa tgtcatatgt gagacggcta gttgaacaaa
     5761 tattaaattt tgctgaattt tttatgttga ttttacttgt tacagaacat gtcacatgat
     5821 atatagataa gattagttgc attaatgatg agggttatta ttcgattcgt atcagattga
     5881 taaatatata aaggtacata gcatgcaaga gcatggcgtt tgtatggcaa agttattata
     5941 agtaactatc gctactccat ttaagttcac tcagaagaac tggtccactt acgttagtta
     6001 ttaagcaaac gttcgctttt ataaacataa tcaggattaa aatgttggat tattgctaac
     6061 ccagcacagc tagtgcgcgt ctgtaattat aagggaaaac gatgaagaat aaggctgata
     6121 acaaaaaaag gaacttcctg acccatagtg aaatcgaatc actccttaaa gcagcaaata
     6181 ccgggcctca tgctgcacgt aattattgtc tgacattact ttgttttatt catggtttcc
     6241 gggcgagtga aatttgccga ttgaggattt cggatattga tcttaaggca aagtgtatat
     6301 atatccatcg attaaaaaaa ggcttttcaa caacgcaccc gctattgaac aaagaaattc
     6361 aggctttaaa aaactggttg agtatccgta cttcttatcc gcatgctgag agcgagtggg
     6421 tatttttatc acgtaagggg aatccacttt ctcggcaaca gttttaccat attgtctcga
     6481 cttccggtgg taatgccggg ttgtcactgg agattcatcc gcacatgtta cgctattcgt
     6541 gtggttttgc tttggcgaat atgggaatag atacgcgact tatccaggat tatcttggac
     6601 atcgcaatat tcgtcatact gtctggtata ccgccagcaa tgcagggcgt ttttatggca
     6661 tctgggatag agccagagga cgacagcgtc acgctgtttt atagagatag ttgagatacc
     6721 agggatggtg ttaaaaacta cattattctt acgatatatc aaaaatgatt aaccattta
//
LOCUS       DAZFFE010000173         6621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFFE010000173 DAZFFE010000000
VERSION     DAZFFE010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.174"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(168..323)
                     /gene="hokD"
                     /locus_tag="ACHJ69_004542"
     CDS             complement(168..323)
                     /gene="hokD"
                     /locus_tag="ACHJ69_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO5017371.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(561..1226)
                     /locus_tag="ACHJ69_004543"
     CDS             complement(561..1226)
                     /locus_tag="ACHJ69_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFO5017372.1"
                     /translation="MTIADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNECGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(1401..1826)
                     /locus_tag="ACHJ69_004544"
     CDS             complement(1401..1826)
                     /locus_tag="ACHJ69_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO5017373.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGCGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDRRLNIICCECRKSEAMQRVLAFYQGNVRYFRRY"
     gene            complement(1867..2949)
                     /locus_tag="ACHJ69_004545"
     CDS             complement(1867..2949)
                     /locus_tag="ACHJ69_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFO5017374.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDKDTDKDTDKDTDQ
                     EDQNTMVHGVKNATNQAGDVQTVNLGQPAGTTPEADSAYALKADSGAVQQVMTARPEQ
                     SHQLQQPEADSAIQREADRVVPENTGQSVGRVDYPDVFEQVWREYPLRAGANPKKSAF
                     SAWKARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRATTFFGPDRNFENPWLLP
                     VSGTNNQRCVNHISEPDNEIPPGFRG"
     gene            complement(3020..3445)
                     /locus_tag="ACHJ69_004546"
     CDS             complement(3020..3445)
                     /locus_tag="ACHJ69_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO5017375.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWIEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(3429..3671)
                     /locus_tag="ACHJ69_004547"
     CDS             complement(3429..3671)
                     /locus_tag="ACHJ69_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO5017376.1"
                     /translation="MKKSEVLGYFGGVVKTAAALGTSKTTVSMWGEEVPWKWALLIQA
                     VTAGALKYELHIPTVVIPGSDHNPPSNQGGIHENQA"
     gene            4063..4401
                     /locus_tag="ACHJ69_004548"
     CDS             4063..4401
                     /locus_tag="ACHJ69_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO5017377.1"
                     /translation="MKNSSITSCVQLVGEIPANTFAVVLESDSMSTSGGGVSIPNGST
                     VFVDPDRTVQPGNIVLALPKGTTTPVIRKLEIEGPDILLVPTNPRYPSIMLDDLSCIL
                     GVCFKIQQDI"
     gene            4690..4845
                     /locus_tag="ACHJ69_004549"
     CDS             4690..4845
                     /locus_tag="ACHJ69_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO5017378.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            4847..4975
                     /locus_tag="ACHJ69_004550"
     CDS             4847..4975
                     /locus_tag="ACHJ69_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017379.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEAHSFNEFKQVLFSSAKSNG"
     gene            5005..5223
                     /gene="ydfC"
                     /locus_tag="ACHJ69_004551"
     CDS             5005..5223
                     /gene="ydfC"
                     /locus_tag="ACHJ69_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO5017380.1"
                     /translation="MQKREPVIIAPDYTDNELYEWMRQKINAAQDLKWANEARAKQAE
                     NLSALEQDITRLEKAAALSIARMITYPR"
     gene            5792..5980
                     /gene="dicB"
                     /locus_tag="ACHJ69_004552"
     CDS             5792..5980
                     /gene="dicB"
                     /locus_tag="ACHJ69_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO5017381.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            5977..6165
                     /locus_tag="ACHJ69_004553"
     CDS             5977..6165
                     /locus_tag="ACHJ69_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO5017382.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            6261..>6621
                     /locus_tag="ACHJ69_004554"
     CDS             6261..>6621
                     /locus_tag="ACHJ69_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO5017383.1"
                     /translation="MSKVFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVK"
BASE COUNT         1668 a         1671 c         1526 g         1756 t
ORIGIN      
        1 cggttgtctc tgctggcgca gcaggtgcca gttgttcagg ctggcgtgcg aattgtaaac
       61 cagaatgcca ggaaaaaaca aaacccgccg aagcgggtta agtgcgggtg cgttgaggat
      121 gcctgacaca tcagaggtgg cgagggattt ctcccccgcc tggtctctta ctcctcaggt
      181 tcgtaagctg tgaagacagc gacctccgtc tggccggttc ggattcgtac ctcgcagagg
      241 tctttcctcg ttaccagtgc cgtcactatg acggttaaac agatgacgat cagggcgatt
      301 aacatcgcct tttgctgctt catagcctgc ttctccttgc ctttcggcac gtaagaggct
      361 aacctacatg tgttcagcat ggattgagcc tcagattaat gttaagcgtc ttgcagaacg
      421 cgtaatgtta actggggctt ttctctatct gccttttggt gttcatgcct gaggcagata
      481 gcctcaagca cccgcagcaa ttctacttaa ctcacgtcac ctcgccaata tgaaatcaat
      541 cagaaaggtg atccataaaa tcactccttc tcttcttttc cgtagtggag ttggccaatt
      601 ttgataagag ggcgtccctg agatttgcgg tgtagattgg tatcgcgcag agaatacaca
      661 cagccacaat attcctgctg atagaatttt tcgcgcttgc tgatttcaat catacgggac
      721 gagccgccct gcttgcgcca gttataatcc cagtatacca tacccggata atgcgcaaca
      781 gctcgccgcc cacactcgtt aacctgctgc atatttttcc agcgcgaaat gcccagtgaa
      841 ctgctgatca cactgaaacc attttcagca gcgtacaacg ctgtccgctc aaaacgcatg
      901 tcaaaacaca tggtacaacg gatccccctc tcaggctccc attccattcc tttggcacgt
      961 tcaaaccagt tgtcggtgtc gtaatcagca tcgataaacg gcacgccgtg ttgttcagca
     1021 aagcgaatat tttcatcctt acgaattaaa tactctttct gaggatgaat gttcgggttg
     1081 tagaaaaaga tggtgtagtc gattcccgag gcctgaagcg cctccatcac ttcaccggaa
     1141 catggagcac agcaagagtg cagtagtagt ttgtttgccc cgtttgggag ctccaattta
     1201 ggccgtttga aatcagcaat agtcataaat atttttattg gggtcatgaa aatagcacag
     1261 agtgtagcat cagagcaggg ctatcgggaa tatatgtcta aatctggtaa tatctggttt
     1321 tgacgcaaag cggacaacca cgctggctct accctgcgcc atgaaaatgt caattcacat
     1381 ctgaactaat gctctttaat ctagtaacgt ctaaaatacc taacatttcc ttgataaaat
     1441 gccagtacac gctgcatagc ttcgctcttc cggcactcgc aacagattat attcagacgc
     1501 ctgtcgtagc ggcgtatttc gccgtctggt aacgaccaga taaggtccgg atcaaccact
     1561 gcaggtttct tcacctttgc ccttgagagt tttttgcggg cattttgcca gtccttacgc
     1621 gcctgttcag acgggaataa tccgcagccg gagttgtata catcgccact ggctaccagt
     1681 tctctggcaa gaacgctcat cagatatctt gttgccccag ttttagcttc cagttgtcgt
     1741 aacgtctcgc gcccactctg gcgtacgagt tcaacaacct gccctttaat tttttcccgc
     1801 tcttcttgtg taaaaacttt tgccacaagc cctcctgaaa attacctcat gaccagaaat
     1861 taacacttac cccctgaagc ccggcggaat ttcgttatcc ggttcagaaa tatgattcac
     1921 acaacgctgg ttgttcgtgc cgcttaccgg gagcaaccag gggttctcaa aattccggtc
     1981 cggtccaaaa aacgtcgtcg ctcgctgaac aaattccgtt cccgttttcc cggtagccgc
     2041 caagtatctt gcgtaacgcc tcacgccatc cagcatggct tctggtggca ccccctcgcg
     2101 taatctggcc ttccaggcac tgaaagcgga tttcttcggg tttgctccgg cacgcaacgg
     2161 gtactcccgc cagacctgtt cgaacacatc cggataatcc actcgtccca cagactgccc
     2221 ggtgttttcc gggactaccc gatcggcttc ccgctgaatg gcggaatcgg cttcgggctg
     2281 ctgcagttgg tgtgattgct ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc
     2341 ggctttcagc gcatacgctg aatcggcttc cggtgtcgtg cctgctggct gaccaagatt
     2401 gacggtctga acatcccctg cctggttcgt ggcgtttttt acgccatgga ccatagtgtt
     2461 ttgatcttct tgatctgtat ctttatctgt atctttatct gtatctttat ctgtcgtgac
     2521 tcgtcgtgac atgtgcgtga catttcgtga cgcgccgtga caatcgccat tttgttcccg
     2581 ctttctttcc ctctctcgct gcgccctctt gcgctctgca ggagattttg cggtttgcga
     2641 aatattgccg ttgtcctctt taagcacctg gcgtttttcc catccagtga ttaaatcacc
     2701 atcaagtacc cgcccctgca tcgtctgcaa aattgaatca attacctctt ctgtcacgtc
     2761 gagcgcactt gccaaatctt ctgtcgtgac atcaatgtga cctcgcgtga catttcgtga
     2821 cgcgctcacc aggaggtgga tatacactgc catcactgtt gcaattggct gccctgacac
     2881 tctggcaatt gttcgccact tagggtcatt tggcatgtca tgccataatc tgagccaggc
     2941 gttagccata ctcacctctt ctgataccga atcttttact cacgagttgc cggaagcgat
     3001 tcgatatggc tattatcact caatgcactg ccacagcatt tcctgccggg ccaccacggt
     3061 tcatctgatt gaaaccggcg attgccactg cgacaaaatc atcagcgtct ctcaccagtc
     3121 gctcccgcgt ctccactagt tcccgaaaat aggctgagct atgactgcgc attcgggcca
     3181 ccagcagagg tggcattgct ttttcgatag ctggtaacaa cgcctgaatt ttttcaactg
     3241 catcaggggt gtctttctct atccagcgga aaattttctg ggtattgcga gccagggctt
     3301 ccggatggct gtcgtcgtac agttcaggaa acgtcatacc caactcaaaa taagcctggg
     3361 ttattccagc tgccggaact ttttcgccat caggacgcgc ccaggcattc atcgccatgc
     3421 ggatgtgttc atgcttgatt ttcatgaatc cccccttggt tagaaggcgg attatgatca
     3481 gaaccgggaa tgacaaccgt cggtatgtgt aactcatatt tgagcgcccc ggcagtgact
     3541 gcctgaatta gcaacgccca tttccacgga acctcttccc cccacatgct gactgtggtt
     3601 tttgacgttc ctagagctgc ggctgtttta acaactccgc caaaatagcc taatacttct
     3661 gattttttca tgagtcgctc cataaaactg aacgccaaaa gtttaataat caaaaccaaa
     3721 gaaagtcaag aaacaaaacc atctgtgttt taaaatcaaa acatgagcaa gcaaacaata
     3781 tctgaacgca taacccaacg tatgcatgcg ctaaacctga aaggcaaaga ccttgtcaat
     3841 gccactggcg catcaaaagg ctccgtaagt caatggatga acggtggagg agcgccgtcc
     3901 tcgcgttaca taagttcact ggcaaaaata ttgaaagtaa acgaaaattg gcttcttaat
     3961 ggaggagagt taaatacagg tgattcgctt gatctatctt taccgccgat aaaaacggtt
     4021 ccgctactat cacttcagca ggcagcaagc tggagtgatt atatgaaaaa ttcctcaata
     4081 acctcttgtg tgcagcttgt cggagaaatc ccggccaata cctttgcagt tgttctagag
     4141 agtgacagta tgtcaacatc tggtggggga gtttccatcc caaatggttc aacagttttt
     4201 gttgatcccg atcgaaccgt acaaccagga aatattgtcc ttgccttacc caaagggacc
     4261 acaacacctg tcattcgtaa actggagata gaagggccgg atattctttt agtccccacg
     4321 aatcctcgct acccttcaat tatgctggat gatctatctt gcatattggg cgtatgcttt
     4381 aaaattcaac aagatattta accgacctca tctatttgat taactgtatg ccatcgtggt
     4441 gatggcttaa cagctgcctg cttagaatgt tttgataaaa aaacattgac ctgaaaagtt
     4501 catttttcta aacttcattc cctctcccca ccccacagaa tgcagggcaa tacttcgagt
     4561 taccaggcag tggtcagggg ttaagtagcc agcccgaggc gtaagaacat gacggcaggg
     4621 ttcaacttta ataactatgc agcaggtttt tgttccgcta ccccggcgtt aaggggaaat
     4681 gaggtcagca tggatactat cgatcttggc aacagcgaat ctctggtatg tggcgtgttc
     4741 cccaaccagg acggtacgtt caccgcgatg acgtatacca aaagcaaaac gtttaaaacc
     4801 gaaaatggtg cccgtcgctg gctggaaaga aactcaggtg agtgatatgg attttgacac
     4861 aatcatggaa aaggcttacg aagaatactt cgaaggcctt gccgaaggcg aagaagctca
     4921 cagcttcaac gaatttaaac aggtgctttt cagttcggca aaatctaacg gctgataagc
     4981 gaaacagcac cgcgaggaat cagtatgcag aaacgagaac ccgtcatcat cgctccagac
     5041 tataccgata atgaacttta tgagtggatg cgccagaaaa ttaatgcagc gcaggatttg
     5101 aaatgggcca atgaagccag ggctaagcag gctgaaaatc tgtccgctct ggagcaggat
     5161 atcaccaggc tggaaaaagc agcggcatta agcattgcca gaatgattac atacccgcgt
     5221 taatagctaa ccaacgaagc taaggttggt aattaagtag ttctccacgg gtgaggtgga
     5281 gtgcgtgcgc cggacgcggg tgagcatccg gcactgacag tttactgaaa ggatatttcc
     5341 ctgaaaagtc agaccataac gcgaaagcgc acggcaaggt agctggttca tagatagcct
     5401 gtcgttaaat tttcgtcgac cgtgcgcttc cggttgtggc aacccgcgaa atggtgcggc
     5461 ggtaagtatg gcggggttat ttcttccccc gttgaggaca ccgggttgtc aggttgacca
     5521 tacgcttaag tgacaacccc gctgcaacgc cctctgttat caattttctg gtgacgtttg
     5581 gcggtatcag ttttactccg tgactgctct gccgcccttt ttaaagtgaa ttttgtgatg
     5641 cggtgaatgc ggctaagcgc acgcggaaca gttaaaacca aaaacagtgt tatgggtgga
     5701 ttctctgtat ccggcgttaa ttgttaactg gttaacgtca cctggaggca ccaggcactg
     5761 catcacaaaa ttcattgttg aggacgcgat aatggaaacg ttattaccaa acgttaatac
     5821 gtctgaaggt tgttttgata ttggtgttct gctcagtaac cgggagttta ctgaagatgc
     5881 cattaatatg aggaaatatg agccttatct gctcaatgat aattccatac tttcccgaat
     5941 tgctcttctt gaacttggta ttttcggaga acgtcaatga cttcagcatt tgcactgatg
     6001 atgacggttt ttcttataac gggtgaatca cagaatgtga ttaccggaat ttatgccagt
     6061 aaagaatcct gcctccaggc aagagacgag caaaaaattt ctggtgaatg cctcccggta
     6121 aaaaaagtat cgctgtacct gaataacgaa acaccggctg gataaccctc cagccatatt
     6181 aacaccatac caacggatta aaaatgccag caatggcagg gattcgttca ccctgaaatc
     6241 tgtaatgagg ttaaaacaaa atgagtaagg tctttatttg cgctgctatt cctgatgaac
     6301 aggccataaa agaagatagc gctgttgcgg tggccactgc cattgaagcc ggtgatgagc
     6361 gtcgcgcacg cgcaaaattt cattggcaat ttctggagca attccctgca gctcaggact
     6421 gcgcttataa atttattgtc tgtgaggata aacccggcat accccgccct gccctcgatt
     6481 cctgggatgc tgaatatatg caggaaaacc gctgggatga ggagtctgct tcctttgtcc
     6541 cggttgagac tgaatcagat ccgatgaacg tcacttttga caagctggcc cctgaagtac
     6601 agaacgctgt catggttaag t
//
LOCUS       DAZFFE010000174         6483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFFE010000174 DAZFFE010000000
VERSION     DAZFFE010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.175"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1450
                     /locus_tag="ACHJ69_004555"
     CDS             <1..1450
                     /locus_tag="ACHJ69_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO5017384.1"
                     /translation="LLDQALFIPHLKCGIVAQDKQAASEIFRTKIAVPFDHLPDWLRA
                     SFTIVERRSGASGGYILFGHGSSIQVATSFRSGTVQRLHISEHGKICAKYPAKAKELR
                     TGTLNAVSDECIIFDESTAEGVGGDFYEMSNRAQEITASGLALTPQDYKFHFYAWWQD
                     LKYSARVPESGLKLSREKTAYFSAVEKAMNITLTDEQKHWYICKETEQREEMKQEFPS
                     TPQEAFLTSGRRVFSAESTLQAESFCSPPLIVYDIEPVTGTKTKAQSLRDGNKAEQHR
                     TLMNYLLVWELPDPDEEYVCGADTAEGLEHGDRSSLDIIRCSNGEQVAHWFGHLDAEL
                     FAHLIAQVCRMYNNAFVGPERNNHGHAVILKLRELYPTRYIYNEQHLDQAYDDDTPRL
                     GWLTTRQSKPVLTEGMKTLLNNGLSGIRWSGTLSEMNTYVYDAKGSMNAQEGCFDDQL
                     MSYMIAQEMRARMPVRVKQKTDKRRTTHWMAH"
     gene            1450..3594
                     /locus_tag="ACHJ69_004556"
     CDS             1450..3594
                     /locus_tag="ACHJ69_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO5017385.1"
                     /translation="MKNETNTMATKNDNGATPRFSQRQLQALCSDIDSQPKWRDAANK
                     ACAYYDGDQLPLEVLQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLLVISDDPDD
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKVGLSWVEVRRNSDPFGPEFKVST
                     VSRNEVFWDWLSREADLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TVTEGQPSPLMSAWEEYQSWDRKENEWLQRERRRVLLQVVYYRTFDRLPVMTLSSGRV
                     VAFDKNNLMHAVAVASGRVQVRVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDNELMEQI
                     ERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QESGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQQAAQQQQALEQ
                     QQQELQMREMAGRVAKLEAEAARAQAAAQRDNAGAQRDVAAAQGQRYVDALNQAHTAE
                     IITGIQNMEQEQEVLQQQMLHTLQQRMHELPL"
     gene            3752..4759
                     /locus_tag="ACHJ69_004557"
     CDS             3752..4759
                     /locus_tag="ACHJ69_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017386.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDGHEQDGVTEVATENHAD
                     EAAQTQTGDNTAAPTPDASAEQTQDEKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRLVELLTSQIHQAGMKPAPLPENEKISDEKIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSAQQAEGHGGEDLSPVLDAMNAVPVLKTWQNSDPDRFSVA
                     VSIDGKLQNDPAWKDKPLTERFAEVARRTQLAFGEASEPPSDDKADNTDIRKTAEEKV
                     KAAEREMAVPASPSELGNTASVGTGDKFEQLLSASHTEIEAAMRGMTDAEIDAILEKL
                     G"
     gene            4782..5996
                     /locus_tag="ACHJ69_004558"
     CDS             4782..5996
                     /locus_tag="ACHJ69_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFO5017387.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQDAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTADHPEFKKIMINDVLPPTHDRHFFGGDATSFEGIDQSDLFTLALVDNLSL
                     FIDEMAHPLQPVRLSGDELYGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMTRAVNRS
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFFTNSKVLVSKNDMAATTEEKQAKTNIDR
                     AMLLGAQALANAYGQKGGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            6078..6440
                     /locus_tag="ACHJ69_004559"
     CDS             6078..6440
                     /locus_tag="ACHJ69_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017388.1"
                     /translation="MSERVYTGTHGNESVAEGVFTVSAAEQETVIHLLSLPAGIRINS
                     LQLVSTGGLGTATVSVKSGEHVLIDNSEAVSAKFAKYVPVEPYTTQSDGELVTVTIKT
                     AAATGTLNVLLRYTVVGY"
BASE COUNT         1648 a         1569 c         1850 g         1416 t
ORIGIN      
        1 tcttctcgac caggcattat tcataccgca tctcaaatgc gggattgtcg ctcaggataa
       61 acaggctgcc agtgaaattt tccgcaccaa aattgctgta ccgtttgatc atcttcctga
      121 ctggctgaga gcctcattca ccatcgttga acgtcgtagc ggagccagcg gtggctatat
      181 cctgtttggt cacggctcga gtatccaggt ggcaacctca ttccgttcag gtacggtgca
      241 gcgcctgcat atctcagagc acggcaaaat ttgcgcaaaa tatccggcta aggcgaaaga
      301 actgcgaacc ggtacactta atgccgtctc tgatgagtgc attatttttg atgaatccac
      361 tgctgaaggt gtgggtggcg atttttacga gatgagtaac cgggcacagg aaatcactgc
      421 atcagggctg gcgctgacgc cacaggatta taaattccat ttttacgcct ggtggcagga
      481 tctcaaatac agcgccagag tgccggaaag cggacttaag ctgtcacggg aaaaaacagc
      541 gtatttttct gcggttgaaa aagcaatgaa catcacgctt accgatgagc agaaacactg
      601 gtacatctgc aaggaaactg aacagcgtga ggaaatgaag caggaatttc cctcaacgcc
      661 acaggaggcg tttctgacgt caggacgacg ggtgttcagt gcggaaagca cgctgcaggc
      721 agagtcattc tgttcgccac cgctgattgt ttatgacatt gaaccggtta caggaacgaa
      781 gaccaaagca cagtctctgc gtgacgggaa taaagccgaa cagcaccgga cgctgatgaa
      841 ttatctgctg gtctgggagc tgccggatcc ggatgaagag tatgtatgcg gagcggatac
      901 cgctgagggg ctggagcacg gagaccgctc atcgctggat atcatcagat gcagtaatgg
      961 tgagcaggtg gctcactggt ttggtcatct cgatgcggag cttttcgctc atctcattgc
     1021 gcaggtctgc cgtatgtaca acaatgcgtt tgtggggccg gagcgtaaca atcacggaca
     1081 tgccgttatc ctgaaactca gggaactcta tccgacgcgt tatatctaca acgaacagca
     1141 ccttgaccag gcatatgatg acgatacgcc ccgtcttggc tggctgacaa cccgccagag
     1201 taaaccggtc ctgacagagg gaatgaaaac gctgctgaat aacggcctgt cggggatccg
     1261 ctggtcgggc acattatcag aaatgaacac ctacgtttat gacgcgaaag gctccatgaa
     1321 tgcacaggaa ggctgttttg atgatcagct tatgagctac atgattgccc aggagatgcg
     1381 cgccagaatg ccggtgaggg taaaacagaa aacggataaa cgcagaacca cacactggat
     1441 ggctcactga tgaaaaatga aactaatacc atggcgacga aaaacgacaa cggagccacg
     1501 ccgcgttttt ctcagcgcca gttacaggcg ctttgttctg atattgacag tcagcctaaa
     1561 tggcgcgatg ccgcaaacaa ggcctgtgcg tattacgatg gtgaccagtt gccactggaa
     1621 gttctccagg tgttgaaaga tcgcggtcag ccgatgacca tccataacct catcgcgcct
     1681 accgttgatg gtgttctggg tatggaggcc aaaacgcgga ctgatctgtt ggtgatatca
     1741 gatgatcctg atgatgaaac agaaaaactg gctgaagcta ttaatgctga atttgccgat
     1801 gcttgccgcc ttggaaatat gaataaagcc cgttctgatg cctatgcgga acaaatcaaa
     1861 gtggggctca gctgggtgga ggtcagacgg aacagcgatc cgttcgggcc tgagtttaag
     1921 gtgtctactg tcagccggaa tgaagtgttc tgggactggc tcagccggga ggctgattta
     1981 agtgactgcc gctggctgat gcgccgccgc tggatggata ccgatgaggc aaaagccaca
     2041 tttccgggaa tggctcaggt tatcgattat gccattgatg actggcgtgg ttttgttgac
     2101 accacggtta ctgaaggcca gcccagtcct ttgatgagtg catgggaaga gtaccagtca
     2161 tgggatcgta aggagaacga atggcttcag cgtgaacgcc gccgcgtgct tcttcaggtg
     2221 gtttattacc gcacattcga tcgcctgcct gtgatgacgc tcagcagtgg cagggtggtg
     2281 gcctttgata aaaataatct gatgcatgcg gtggcggtgg catccgggcg ggtgcaggtg
     2341 agagtcgggc gggtaagtcg tattcgtgaa gcctggtttg tggggccgca ttttattgtg
     2401 gatcgcccct gtagtgcgcc acaggggatg tttccgctgg ttcccttctg gggataccgg
     2461 aaggataaaa ccggagagcc ttacgggctg atttcccggg ccattccggc acaggatgag
     2521 gtgaattttc gtcgtataaa gctgacgtgg ttgcttcagg ccaaacgtgt gattatggac
     2581 gaggatgcca cccagttgtc agacaacgag ctgatggagc agatcgagcg tccggatggc
     2641 attattaaac tgaacccggc ccgaaaaaat cagaaaagcg ttgctgatgt tttccgggtt
     2701 gaacaggatt ttcaggttgc cagccagcag tttcaggtta tgcaggagtc ggaaaaactt
     2761 atccaggaca cgatgggggt ttattccgca tttctcgggc aggaatcagg cgcaacttcc
     2821 ggcgtggcga tcagcaacct ggtggagcag ggagccacaa cccttgccga aatcaacgat
     2881 aactaccagt ttgcctgcca gcaggtggga aggctgttgc tcgcttatct tctcgatgac
     2941 ctgaaaaagc ggcgtaatca tgcggtggtg attaatcgcg atgatcgcca gcgtcgccag
     3001 accattgttc tcaatgctga aggtgataat ggtgaactga ccaatgatat ttcaaggtta
     3061 aatacacata ttgcgctggc tcctgttcag cagacaccgg cgtttaaggc acagcttgcg
     3121 cagagaatgt cagaggttat tcaggggctg ccacctcagg tgcaggccgt cgtgctcgac
     3181 ctgtgggtta atcttctgga tgtgccgcag aaacaggaat ttgtcgaacg tattcgtgcg
     3241 gcgctgggga caccaaaatc accggatgag atgaccccgg aagagcagca ggccgctcag
     3301 cagcaacagg cgctggagca gcaacaacag gaactgcaga tgcgcgaaat ggcaggccgg
     3361 gtggctaaac tggaagcaga agctgccagg gcacaggcag ctgcgcaacg ggataatgct
     3421 ggcgcacaac gggatgtcgc cgcggcacag ggacagcgtt atgtggatgc gctcaaccag
     3481 gcgcatacgg cagaaatcat taccggcata cagaatatgg aacaggagca ggaagttctt
     3541 cagcaacaga tgcttcatac actacaacag cggatgcatg aattgccgct ctgaatatcc
     3601 tgaacttaac ggaaccccgt catcgtacgg ggttttttgt ctccggagat aagcgttccg
     3661 ggagcggtgc gcttattcgc gggggcagcg ataagcctta tttactcaac cattcggatc
     3721 tgtccgataa acagaccatg cggagttatt tatggatttt gaatttacgg gtgaagaaac
     3781 cccggaacaa ctggaaaaaa tgctggaagg gcttggggat gtggatattg acggtcacga
     3841 acaggacggc gtgacagagg tcgccacaga aaatcatgcg gatgaggcag cacagacaca
     3901 gacgggcgat aacactgcgg caccgacgcc ggatgccagt gctgagcaga cgcaggacga
     3961 gaaggagccg gaggcgaagg gtgtgctcac ccgcgatggt aaacacgtca ttccctatga
     4021 agtccttgag gctgaacgtt ccggtaagca gcgggccgaa caggaagccg cacttcttcg
     4081 gggacagata gccgaagaaa aacgcctggt tgaactgctg acgtctcaga tccaccaggc
     4141 cggtatgaaa cccgcaccgt taccggaaaa cgaaaaaatc tctgatgaga agattgcccg
     4201 tatcagggag atgtatccgg aaattggtga cgcggtggct tcgctcatcc gtaaaaataa
     4261 ctatctccag tcccgtgttc agcaatcagc acagcaggca gaaggtcatg gtggtgagga
     4321 tttatcaccg gttcttgatg cgatgaatgc cgtgccggtg ctgaaaacgt ggcagaactc
     4381 cgatccggat cgcttctcgg ttgccgtatc catcgacggg aagctccaga atgaccccgc
     4441 atggaaagac aaaccgctca ctgaacgttt cgccgaagtg gcccgccgta cgcagctcgc
     4501 tttcggtgaa gccagtgagc cgccttctga tgacaaggca gacaacacgg atatccggaa
     4561 aacggcggaa gaaaaagtga aagccgctga acgggagatg gcagtgcctg cttccccgtc
     4621 agagcttggc aacacggctt ccgtcggaac cggtgataaa tttgaacagt tactgagtgc
     4681 ttcccatacc gaaattgaag cagccatgcg aggcatgacg gacgctgaaa ttgacgcgat
     4741 tctggaaaaa ctcgggtaac cactgaagga gtactgacgt aatgaccaca gtaacatcag
     4801 cccaggcgaa taagctgtat caggtggcgc ttttcacggc tgccaaccgc aaccgctcaa
     4861 tggtgaatat actcactgaa cagcaggatg caccaaaagc agtttcgcct gacaagaaaa
     4921 gcacgaagca gaccagtgca ggtgcaccgg ttgtgcgaat cacggacctc aacaaacagg
     4981 ccggtgatga agtgacgttc agcatcatgc acaaactctc taaacgtccg acgatggggg
     5041 atgagcgcgt tgaaggccgt ggtgaagacc tcagccatgc tgatttttcc ctgaaaatta
     5101 atcagggccg tcacctggtg gatgcgggcg gacgtatgag ccagcagcgt acgaagttca
     5161 acctggcatc ctctgccaga acgctgctgg gtacgtactt taacgacctg caggaccagt
     5221 gtgcgatagt gcatcttgcc ggtgcccgcg gtgattttgt tgctgatgac accattctgc
     5281 caacggcaga tcaccctgaa ttcaaaaaaa ttatgatcaa cgacgtactg ccgccgacgc
     5341 atgatcgtca ttttttcggt ggggatgcga cgagttttga aggtattgac cagtctgacc
     5401 tgttcactct tgcgctggtc gataatctct cactgtttat tgacgagatg gctcatccgt
     5461 tacaaccggt acgcctgtcc ggtgatgagc tgtatggcga agatccgtat tacgtcctct
     5521 acgtcacgcc gcgtcagtgg aatgactggt acacctccac gtccggcaag gactggaacc
     5581 agatgatgac gcgtgccgta aaccgctcaa aaggttttaa ccatccgctg ttcaaaggcg
     5641 aatgtgcgat gtggcgcaat attctggtcc gtaaatatgc ggggatgccg atccgtttct
     5701 ttacgaactc aaaagttctg gtatcgaaaa acgatatggc agcaaccaca gaggagaagc
     5761 aggccaagac caatatcgac cgtgccatgc tgctgggcgc tcaggcgctg gcgaatgcgt
     5821 acggtcagaa gggcggcggt cacttcaaca tggttgagaa gaaaacggat atggataacc
     5881 gtactgagat agcaatcagc tggatcaacg gcctgaaaaa aatccgtttc ccggagaaga
     5941 gcggcaagat gcaggatcac ggcgtgattg ccgttgatac cgcagtgaag ctctgatttt
     6001 tcctttcccc atgccgggtt atcgcccggc tttttcagga gtcattaatt atggcaaaga
     6061 ctatccttgc tccgtaactg agtgagcggg tctatacggg tacgcacggt aatgagtcgg
     6121 tggcagaagg tgtgtttaca gtgagtgctg ccgaacagga aaccgttatt catcttctct
     6181 cactgccagc tggcatccgt atcaactcac tccaactggt ttcaacgggc ggtctgggta
     6241 ccgcaaccgt cagcgtcaag tccggtgagc atgttctcat cgataacagc gaagctgttt
     6301 ctgcaaaatt tgctaaatat gtgccagtgg agccgtacac cacacagagt gacggggagc
     6361 tggttacggt caccattaag acagctgctg cgaccggtac cctgaatgtt ctgctgcgtt
     6421 ataccgtggt gggatactga tataaacctt ccggcccgcg tcatgcgggc ttttttatcc
     6481 ggg
//
LOCUS       DAZFFE010000175         6268 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFFE010000175 DAZFFE010000000
VERSION     DAZFFE010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.176"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..315
                     /locus_tag="ACHJ69_004560"
     CDS             <1..315
                     /locus_tag="ACHJ69_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5017389.1"
                     /translation="SPAQYLQRQKPQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGH
                     GLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKW
                     KA"
     gene            316..681
                     /locus_tag="ACHJ69_004561"
     CDS             316..681
                     /locus_tag="ACHJ69_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5017390.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     GQLVLGGRLGVKIYKIESE"
     gene            690..1250
                     /locus_tag="ACHJ69_004562"
     CDS             690..1250
                     /locus_tag="ACHJ69_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO5017391.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRRMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKLKMAERLNADHPEWTLVTCRRRIDQWL
                     GISEFMLHAPMRMAFVTEKKMLQTDQ"
     gene            1468..1665
                     /locus_tag="ACHJ69_004563"
     CDS             1468..1665
                     /locus_tag="ACHJ69_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO5017392.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            1816..2874
                     /locus_tag="ACHJ69_004564"
     CDS             1816..2874
                     /locus_tag="ACHJ69_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO5017393.1"
                     /translation="MFNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDARAALGITAKQIVDATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            2915..2990
                     /locus_tag="ACHJ69_004565"
     tRNA            2915..2990
                     /locus_tag="ACHJ69_004565"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2948..2950,aa:Ile,seq:cat)
     gene            3000..3076
                     /locus_tag="ACHJ69_004566"
     tRNA            3000..3076
                     /locus_tag="ACHJ69_004566"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3034..3036,aa:Arg,seq:tcg)
     gene            3090..3166
                     /locus_tag="ACHJ69_004567"
     tRNA            3090..3166
                     /locus_tag="ACHJ69_004567"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3124..3126,aa:Arg,seq:tct)
     gene            4138..6120
                     /locus_tag="ACHJ69_004568"
     CDS             4138..6120
                     /locus_tag="ACHJ69_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5017394.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVQPSGDGGAVISTTSEPEYYFVIALAGQSNSMSFGEGLPLPDTY
                     DRPDPRIKQLARRSTVTPGGAACAYNDIIPADHCLHDVQDVSNLNHPKADLNKGQYGC
                     VGHALHVAKKLLPFMPARAGILLVPCGRGDSGFTAGAEGAFNEASGATAGSSLWGVDK
                     PLYRDLVSRTRAALKKNPKNVLLSVIWMQGEKDVSSGRHAEHNALFLAMVNKYRADLA
                     DIADQCIGGTTSGVPWICGDTTYDWKAKYAVQYEAVYGGYKGKAAQNIHFVPLMTDEH
                     GANVPTNEPSEDPDIITAGYYGAASRSNGNWTTADRKTHFSSWARRGIVSDRLAGEIL
                     VRAGRLLPFLSGQSAPLATTPASTGDAQSGSAGPTQQQGAGTSAGGHTEAVTRMVAGY
                     DANSGSGVWTEQQWNASGGKGTVTDDSGRKALRLEKQPGKLTSWKMFRTVAVEEAKNL
                     LSKGGEIAVRFKIPEGVELVNGQFVFGLYWPVSQWASGATANSMLASFFLQTDASNLN
                     LMYHKGESNAQLGTFGAFDHNWHTVVFRFAGNNSEKVVPVIDDTEQSAFDLVMWTNDG
                     FTADTLTLTDITGAKATYPVLLDTVTVKVNESRASS"
BASE COUNT         1587 a         1383 c         1814 g         1484 t
ORIGIN      
        1 tctccggctc agtatctcca gcgccagaaa ccacaacggg aagagatgcc tgtatacacg
       61 cgctgggtaa aaacgcagaa atgcatgacg tgtggcaatc aggcagatga tccgcatcac
      121 atcattggtc atggactggg agggatggga acaaaggctg acgatttgtt tgttattccg
      181 ctgtgccgta aatgccataa cgaactacac gccggagtaa aagattttga agagaaacac
      241 ggcagtcagc tgttgttgct gattcgtttt ttaatgcacg cgagaaattc gggtgttctg
      301 aagtggaaag cataaatgac cgaacgcata gaatttgttt tgccttaccc gccgacggtg
      361 aacacttact ggcgacgccg tggcagcaca tattttgtat caaaagccgg ggagcgttat
      421 cgccgggcag tggcgcttat tgttcgccag cagcgcttga aattaagcct gtccggacgg
      481 ttggcaataa aaattattgc agaaccaccg gataagcgcc gccgtgacct ggacaatatt
      541 ctgaaagcac cgctggatgc gcttacgcat gcggggttgt taatggacga tgagcagttt
      601 gatgaaatca atattgtacg tggtcagctt gttcttggtg gtcggctggg tgtgaagatt
      661 tacaaaattg agagtgagtg agcgtaaata tgatatatcc ggaaattaca ggcaaaagcg
      721 gcgaacattt acgcctgaac acgctggaag cagtctggat ccaggggaaa ttacggatgt
      781 gggggcggtg gtcgtatatc ggtgggggta aatccggaaa tatgtttaac cggttactgg
      841 tttcgaaaaa gctgacgaaa acagcagtta atgaggtttt acgcagaatg aagaaatccg
      901 ggctggaaaa accggaactt gaggcatttt ttcgggatat gaccagaggg aagcagaaga
      961 gctggttgtc acattgtaca gacacagagg cgttgattat tgatcgcgtt atcagtgagg
     1021 tgcttgggga atatcccgga ctaatcaata ttctccggca aaggtacgaa ggacggggaa
     1081 tgagcaaact gaaaatggcc gaaaggttaa atgcagatca tcctgagtgg acgttggtta
     1141 cgtgcagacg ccgaattgat cagtggttgg ggatatctga atttatgtta catgccccca
     1201 tgcgtatggc ttttgttaca gagaaaaaaa tgttgcaaac tgatcaataa actgcttcaa
     1261 tccgtataag cttcgcaagg ctgtatcgcg aggcgaaatg caagtttttt tcgcacaagg
     1321 aagccaccgg aaggtggttt ttttgtgtcc gtaatataca gcagcgcaat aaattcgctg
     1381 gtggttatta ataccgttct ttcagcttgc tggctttttc gacaagagtt attggtgtgt
     1441 cacgttaacc ggaaaaggga aaaagacatg ctgaaacagc aggatatgac cgaaaccgcc
     1501 agagtggtgt ttaatgaatt aagcgtcacc gaaccggcga cagtcgggga gattgcacag
     1561 aatacgtacc tttcacgcga acgctgtcag ttaatactga cccagcttgt tatggcgggg
     1621 ctggcagact atcagttcgg ttgttacaga cgccttcagt cctgaaggct tttttatttg
     1681 tggtaaatgg gcggctggtg ggtgttaggg gcactcacca gccatctgct catgcgtccg
     1741 gatcacaagc aaacctcagg cccactgctt tgcgcaaaag cagaatgagc ctatcagaga
     1801 caggcttaat gatccatgtt taacactgta aaaatatcca gttgtgagtt aatcaacgcc
     1861 gactgcctgg aatttatccg gtcgttaccc gaaaattctg ttgacctgat agtcacggac
     1921 ccgccgtact ttaaagtgaa gcctgagggc tgggataacc agtggaaggg cgacgatgat
     1981 tacctgaaat ggctggacca gtgtcttgcg cagttctggc gggtgctgaa acctgccgga
     2041 agtctttacc tgttctgtgg ccatcgcctg gcatctgata tcgaaatcat gatgcgtgaa
     2101 cgcttcagtg tgctgaacca tattatctgg gcgaagccgt ccggacgctg gaacgggtgc
     2161 aacaaggaaa gcctgagggc gtatttcccc gccacagagc gcattctgtt cgcggaacat
     2221 tatcaggggc cgtatcgccc gaaagatgcc gggtatgagg cgaagggcag ggcactgaaa
     2281 cagcatgtga tggctccgct gattgcttac tttcgtgatg cgcgtgctgc cctggggata
     2341 acggcaaaac agatagtgga tgccacagga aagaaaaaca tggtgtcgca ctggttcagt
     2401 gccggtcagt ggcagctacc gaacgaaagc gattatctga aattacaggc gctgtttgcc
     2461 cgggtggcag aagagaagca tcagcgcggt gaactggaaa agccccacca ccagctgctg
     2521 gagacgtata cttcactgaa ccggcagtat gcggaactgc agagtgaata taagcatctg
     2581 cggcggtatt ttggcgtaac tgcgcaggtg ccgtacacgg atgtgtggac gcataaaccg
     2641 gtgcagtact atcccgggaa acatccgtgc gaaaaaccgg cagaaatgct gcagcagata
     2701 atcagtgcga gcagtcgtcc gggtgacctg gttgcagatt tcttcatggg gtcgggttcg
     2761 acagtcaaag ctgcgatggc gctggggcgt cgtgcaactg gcgttgagct ggagactgaa
     2821 cgttttgagc agacggtcag ggaagtacag gatttagtca gtcagaatgg atgatattgc
     2881 aggattagtt acgtaccgtt attatcctgc gcccggccct ttagctcagt ggtgagagcg
     2941 agcgactcat aatcgccagg tcgctggttc aaatccagca agggccacca tatcacatac
     3001 cgccattagc tcatcgggat agagcgccag ccttcggagc tggctgcgcg gggttcgagt
     3061 cctcgatggc ggtccattat cagcatcatg cgttgttagc tcagccggac agagcaattg
     3121 ccttctaagt aatcggtcac tggttcgaat ccagtacaac gcgccacact tattttcctg
     3181 gctcgcttcg gcgggccttt taatttgctg aaaaagaaaa catcagatgg ttagtcgggt
     3241 atcagttatc tggtgaaatt tttaaatacc tcacaattca ggaaggtgat tattgttttt
     3301 ctggtgggga atttgttaaa aatcactccg catgatgaat ccccctaagc ggtggggcga
     3361 ctggcaaggc tccattacga tggtcacaat ccgcagattt agtttgccag gctgaatcta
     3421 ccgggaggca cccggcatca tgcataatgt ggaaaattgg aaatgtattc aggcctctac
     3481 gaattttagt agaggctttt ttttgagtaa aaaaagcccg tctatggcag cgggcaagta
     3541 tgcatgagaa tacatttttt attatttgcc tgaaaatagt accctgaggt tattgttgtt
     3601 ttcaacaggc tgatttgtgt tctgatcggg gatgtgacaa ggaacatacg ttcttatgtg
     3661 aaaatgttaa attcttcaca tttcagtgag ttgagtgttg atttcgtttt atcttttttg
     3721 ttaaaaaagt attgcatggt gaatccccct gtgcggtggg gcgactggtg acaacaattc
     3781 atcatccgtg atgacccaga agagatcgcg ggttcagtgg caccgggctg aactcaccgg
     3841 gaggcacccg gcaccatgtt catggtgata cagaaatgcg gcttcagccc ctctccggag
     3901 gggctttctt atggacaaaa aagcccgcgc tgggagacgc gggcggcaag gaataaacaa
     3961 taaaacgtga agtaatattt cagctggcga ataatacccc atagtaatca ctctgcgcaa
     4021 ctgcgcggcc tttttcgtat tgcgggctgt agtctccctt ctgccattgt cctgtaactt
     4081 ccggacttca gctcgctcct tatctgactc acaacattat cccgaccggg aggattcatg
     4141 acatttaaac actacgatgt ggtcagggcg gcgtcgccgt cagaccttgc ggagcgactg
     4201 acacaaaaac tgaaggaggg gtggcagcca tttggcagcc ctgtcgccat cacgccctat
     4261 accctgatgc aggccattgc ggcggaaggt gatgtcacca caccggtggt ggtgcaaccg
     4321 tcgggtgatg gtggcgctgt tatcagcacc accagcgaac cggaatatta ctttgtcatt
     4381 gcgctggccg ggcagtccaa ctcgatgtct tttggcgagg ggctgccgct gccggatacg
     4441 tatgatcgtc ctgacccgcg tattaagcaa ctggcgcgtc gcagcacggt gacaccgggc
     4501 ggtgcggcct gtgcatataa cgacattatt cctgcagacc attgtctgca tgatgtgcag
     4561 gacgtgagta atctgaatca cccgaaagca gacctcaata aagggcagta tggctgtgtg
     4621 gggcatgccc tgcatgtggc caaaaaactg ctgccgttta tgcctgcccg tgcggggatc
     4681 cttcttgtcc cgtgtggacg tggcgattcg ggatttactg cgggagcaga gggcgcgttt
     4741 aatgaggcgt cgggtgcgac agcgggctct tccctgtggg gggtggataa accgttgtat
     4801 cgtgacctgg tcagcagaac gcgtgcagcc ctgaagaaaa atccgaaaaa cgtgctgttg
     4861 tcggtgatct ggatgcaggg ggaaaaagat gtcagttcgg ggagacatgc agagcacaat
     4921 gcactttttc ttgccatggt aaataaatac cgtgcagacc tggcagatat tgcagaccag
     4981 tgtattggcg ggacaacgtc cggcgtcccg tggatttgcg gtgacaccac gtacgactgg
     5041 aaggcgaagt atgcagtgca gtatgaggcg gtttacggag gctataaagg caaggcggcg
     5101 cagaatattc actttgtgcc gttgatgacg gatgagcatg gtgcgaatgt gccgacaaac
     5161 gagccgtcag aagatccgga cattatcacg gcgggatact atggtgccgc gtcacgcagt
     5221 aatggtaact ggacgacagc cgatcgtaaa acgcacttca gctcctgggc gcgaagaggc
     5281 attgtttcag atcggctggc aggagagata ctggtgcgag ccgggcgttt gctgccgttc
     5341 ctgagcgggc agtctgcacc gctggcgacc acgccagcct ccacggggga tgcacagtct
     5401 ggctctgcgg gtccgacgca acagcagggt gcaggtactt ctgcaggcgg tcatactgaa
     5461 gccgtaacaa gaatggtggc cggatatgat gcgaacagtg gcagtggtgt atggacagag
     5521 cagcagtgga atgcgtccgg tggtaaaggc actgtgacgg atgacagtgg caggaaggcg
     5581 ctgcgactgg aaaaacagcc gggtaaactg acctcctgga agatgttccg tactgttgcg
     5641 gtggaggagg caaaaaatct tctcagtaag ggaggtgaaa ttgccgtgcg gtttaagatc
     5701 ccggagggtg tcgaactggt taacggtcag tttgtctttg gtctgtactg gccggtgtcg
     5761 cagtgggcgt caggcgcgac agcaaacagc atgctggcgt ccttcttcct tcagacggat
     5821 gcatcaaatc tgaatctgat gtaccacaag ggggagtcga atgcgcaact gggcacattt
     5881 ggggcatttg accataactg gcatacagtt gttttccgct ttgcgggaaa taacagcgaa
     5941 aaagtggtgc cggtgattga tgatacagag cagtctgcgt ttgacctggt gatgtggaca
     6001 aatgatggct ttacagcaga tacgctgacg ctgacagata tcacgggggc aaaagcgacg
     6061 tatccggtac tgcttgatac ggtcacagtc aaagttaacg aaagccgggc atcatcataa
     6121 ccggcaaaaa aaaccgccag cggcaggaac ggaagctggc ggaggtaatc ccaatggaga
     6181 atgtaaagaa aagatgcttt cgtatatcgg ttttttaaat gaaaacagtt ctcattgtca
     6241 accataacgg taagaaacta tgacattt
//
LOCUS       DAZFFE010000176         6192 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZFFE010000176 DAZFFE010000000
VERSION     DAZFFE010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.177"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            19..3348
                     /locus_tag="ACHJ69_004569"
     CDS             19..3348
                     /locus_tag="ACHJ69_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009485633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO5017395.1"
                     /translation="MSNVLLFLVIVAFVGGIIWRFGDHFGLKGDENNFFAWLICIMTL
                     IFVRASWYFSRLYRLRKEQHHSEKERLRPEKEARIEYGKVITLNIELAEYLCALYGPF
                     WRRKIRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPSAEPDVTLLAALKKL
                     RRSRPLDGIIWPLTEEQSRQTAQLDKGWRELINGSKRLGFQAPLYLWQVCDNGDYQTG
                     RPLQSVGCLLPERCNPEQLAAMLEAQTLPLTEQGMSQLLTDNRHDFLLRLAHTLAERG
                     IAHWQSILKPLLAGGAFSSLRLRGLMFSPPLTAVPEAAPHAWLPSPVWAGVTGDNARG
                     RTVGFPWLRTALIIGICILAIWGAGMTTSFFANRALVQETGIQTARALDTRLPLSEQL
                     VALHTLQGELERLQYRIREGAPWYQRFGLERNQQLLAAAFPGYAQAANRLVRDVAVDH
                     LQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTLMADGLRYE
                     NIPEGVRQSVLPSLLTFWTANLPEHPQWKTSPPPELTGAVRKILLRQIGVRNAENTLY
                     QNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVTA
                     RREEIDWVLSDRQQDTSADISPDTLRNRLTSRYFTDFAGSWLAFLNSIRWKKEDSLSG
                     ILDQLTLMADARQSPLIALTDTLAWQAATGRENRGLSDSLAKSAQELFNGKEKTPQQS
                     REGNEPVGPLDKTFAPLLRLLGDKAGGGGGDTQLSLQTYLTRVTRVRLKLQQVTNAPD
                     PQEMTQQLAQTVLQGKTVDLTDTRDYGRLIAASLGEEWSGFGQALFVRPVEQSWRQVL
                     TPAADSLNRQWQRAIVSHWNQDFAGRYPFKASQNDASLPLLAQYLRDDGRINQFIAAN
                     LSGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGIHFELRAKP
                     ARDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRG
                     SWSLIRLLEQAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLPG
                     QVFLTGRSMKDAEEYGEDADE"
     gene            3341..4951
                     /gene="tssA"
                     /locus_tag="ACHJ69_004570"
     CDS             3341..4951
                     /gene="tssA"
                     /locus_tag="ACHJ69_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO5017396.1"
                     /translation="MSELLQKLTRSCFADRDALDVALTQAALWQTWLLPVTADTPVGE
                     DPGYHDDFLRIRDEMNKLSGTDTGLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLTGLVERFGTQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKDDFANI
                     VAALNQLTVSFAAWPEDQHSPSLMPLINALESRLAQSGGMNAVVPQNSSGVPAPSSPV
                     DAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSSGVSHFWLDIQWYLHQALAKAGAP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDGTPFADEVTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALEMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMALLLLEELDIATQGITLTQWEPELLFEVKARQLKLLRLRAHRYADKALLN
                     RKMDALLGTLVAINPVRAAVLCDTQHKE"
     gene            4951..>6192
                     /locus_tag="ACHJ69_004571"
     CDS             4951..>6192
                     /locus_tag="ACHJ69_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO5017397.1"
                     /translation="MTMGRGYFLVRGDKTTCGGKIIEGADDHTIMGIPQARDMDRMTC
                     GKHPGMFIIVGGVPETGIHGRLMAGSLDSQSSCPCKARFIASMMDDVYEVETEKTENK
                     SVMPLNPLNNGTGNSSPPAYTSDYPALRNTHNLPDQQVRDMLANNNHDVMLLTYEEGA
                     EVLQSWGCQDFKRKWVDITQSAPGQLIINYGVNGKDVVTTSIVLARLGDFGIKATAYV
                     NHKGTELIKLTGYPGIRKILNAPVFAAKNPKIVDVGIGKYGLTNSIVKGARLTFYVAA
                     AYRTIDFILNDETSLAEFIGALATDVGKIAIASAVSWGAGKALLATGFVAGPLVGVVV
                     VGIGAALLLNYLDNKFGLTDQVVAYIEAAQQEFVEKAREIEEGIWDLGAMWAKHMLIK
                     GKEVLEHEVKEYLKDKLNNVSK"
BASE COUNT         1420 a         1638 c         1895 g         1239 t
ORIGIN      
        1 aataaaaaaa gtgtttttat atctaatgta cttttattct tggttattgt ggccttcgtg
       61 ggggggatca tctggcgttt tggtgatcat tttggactga aaggggatga aaataatttt
      121 tttgcgtggt tgatatgcat tatgaccctc atttttgtac gggcatcctg gtatttctct
      181 cgattgtatc gcctacgaaa ggaacaacat cactccgaaa aggaaagatt acgcccggag
      241 aaagaggctc gtattgaata tggcaaggtc ataaccctga acattgagct agctgagtat
      301 ttatgtgccc tatacggccc attctggcgg cgtaaaatcc gcctgctgct ggtgaccggc
      361 gagcctgaac aggcagaggc catcgcgccg gggctgaccg ggcaacactg gctggagggc
      421 gaccacacgg tgctgatata tggcggcagg ccatcagcgg agcctgatgt cacactgctg
      481 gccgccttaa aaaaactgcg ccgcagccgt ccgctggacg gcatcatctg gccgctgaca
      541 gaagaacaaa gccgccagac agcgcaactc gacaaaggct ggcgcgaact gataaacggc
      601 agtaagcgac tcggttttca ggccccactc tatttgtggc aggtctgtga caacggtgat
      661 tatcagaccg gacgccccct gcaaagcgtt ggctgtctgc tgccggaacg ctgtaacccg
      721 gaacaactgg ctgcaatgct ggaagcacaa accctgccgc tgacggaaca gggcatgtcg
      781 cagctgctga ccgataaccg ccatgatttc ctgctgcgtc tggcccatac ccttgcagag
      841 cggggtatcg ctcactggca aagtatcctg aaaccgctgc tggcaggcgg cgcattttct
      901 tccctgcgcc tgcgtggcct gatgttcagc ccgccgctta ccgccgtgcc ggaggccgca
      961 cctcatgcgt ggctgccgtc gccggtctgg gcgggcgtga cgggtgacaa cgcacggggt
     1021 cgcacggtgg gctttccgtg gctgcgtacc gcgctgataa tcggcatctg catactggcg
     1081 atatgggggg ccggaatgac gacctcgttc ttcgccaacc gcgcgcttgt tcaggaaacc
     1141 ggtatccaga cggcacgtgc gcttgatacc cgcctgccgc tgtcagaaca actggtggcg
     1201 ctgcataccc tgcagggcga actggagcgc ctgcaatacc gtatccgtga gggtgcgccg
     1261 tggtatcagc gttttggcct tgaacgtaac cagcaactgc tcgctgccgc ttttcccggc
     1321 tatgcgcagg cggcaaaccg gctggtgcgc gacgtggccg ttgaccatct gcaacagcaa
     1381 cttaatgcct ttgtcgcgct gccgcccaac agtcctcagc gtaccgccac cggtgaacaa
     1441 cgctataagc agcttaaggc attgctgatg acttcccgcc cggaaaaggc cgacgctgcc
     1501 tttttcagta ccacgctgat ggcggacggt ctgcgctacg agaatatccc ggaaggtgtg
     1561 cggcagagcg tgttgccgtc actgctgacc ttctggacgg cgaacctgcc ggaacacccg
     1621 cagtggaaaa catcgccgcc accggaactg accggcgcag tgcgtaaaat cctgctgcgc
     1681 cagattggtg tgcgtaatgc cgaaaacacc ctctaccaga acgtgctgca acaggtgtcc
     1741 cgcaactacg ccgatatgac gctggcggac atgaccgggg ataccctcac cgaatctctt
     1801 ttcagtacgg aacagacggt gccggggatg ttcacccgtc aggcgtggga aggacaggtc
     1861 agggaagcca tcgagcaggt ggtgacggcg cggcgcgagg aaatcgactg ggtactcagc
     1921 gaccgccagc aggatacctc tgcggatatc tcgccggata cgctgcgtaa ccgtctcacc
     1981 tcacgctact ttaccgactt tgccggaagc tggctggcgt ttctcaacag cattcgctgg
     2041 aaaaaggaag actcgctctc cggcattctc gaccagctga cactgatggc cgatgcccgt
     2101 cagtcgccac tgattgcgct gacggacacc ctcgcgtggc aggcggcgac aggcagggaa
     2161 aaccgtggcc tgtcagactc gctggcgaaa tcggcacagg aactgtttaa cggcaaggag
     2221 aaaacgccgc agcaatcccg tgaaggcaac gagcctgtcg ggccgctgga taaaaccttc
     2281 gcgccgctac tgcggttgct gggcgataag gcaggaggcg gtggtggtga tacgcaactc
     2341 agcctgcaaa cgtatctgac ccgcgtcacc cgcgtgcgtc tcaaactgca acaggtgacc
     2401 aacgcccccg acccgcagga gatgacccag caactggcgc agacggtttt acagggcaaa
     2461 accgttgacc tcaccgacac ccgcgactac ggacggttaa tcgccgccag tctgggcgag
     2521 gaatggagtg gcttcggtca ggcgctgttc gtccgcccgg tggagcagtc gtggcggcag
     2581 gtgctgacgc ctgcggcgga cagcctgaac cgtcagtggc agcgggcgat tgtcagccac
     2641 tggaatcagg acttcgccgg gcgctatccg ttcaaagcct cacaaaacga tgcctctctt
     2701 cccctgctgg cacagtatct gcgcgatgac gggcgcatca accagtttat cgccgccaac
     2761 ctttccggtg ttctgaaacg tgaaggccgc tactgggtgg ctgacgccat gaacacgcag
     2821 gggctgacgg ttaacccgga ctttatccgc gccctgaacc gcctgcgtga cgtggccgac
     2881 accgcctttg ccagcggcga tgcgggaata catttcgaac tgcgggcaaa accggcgcgt
     2941 gacgtgatga aaacgcatct ggtgattgac gggcaggagc tggaatattt caaccagaag
     3001 gaacgctggc agcgtttcaa ctggccggat gaacagtggc aacccggcgc atcgctgagc
     3061 tggaccagca cgcaggcgat ggaacgcatt ctggcggatt accggggaag ctggagcctt
     3121 attcgcctgc tggaacaggc gcaggtgaca ccggtggaca gcagcacctt taaggtggtg
     3181 tggaaagcgc aggatggcct gccgctgaat tacctgctgc gggttgaaca gggtaaaggg
     3241 ccgctggcgc tgctggagct gaaaaacttc cgcctgccgg gacaggtatt tctgaccggc
     3301 aggagtatga aggatgcgga agagtatggg gaagacgccg atgagtgaac tgttgcaaaa
     3361 gctgacccgt agctgttttg ccgaccggga tgcactggat gtggcgctaa cacaggcggc
     3421 gctgtggcag acatggctgc tgccggtgac ggcagacacg ccggtcgggg aagaccccgg
     3481 ctaccacgac gatttcctgc gtatacgcga cgaaatgaac aaactttccg gcacagatac
     3541 cggccttatc tgccagcttg ccgaatccct gctgctgacg caggccaaag acgtgcgcat
     3601 cgccacttac tacatctggg cgcgactgca ccgcgacggt gagcgcggac tggcggaagg
     3661 actagcgctg ctgaccggac tggtggaacg cttcggtacg caactgctgc cctcgcgacc
     3721 ggccagcagg aaaatggcgc tggagtggct ggcgggggaa aagatgctcg acagccttgc
     3781 ccgctacccg gaagtggcaa aagatgattt tgcgaacatt gttgccgctc ttaatcaact
     3841 gacggtttcc ttcgccgcat ggcccgaaga ccagcattcc ccctccctga tgccgctgat
     3901 taacgcgctg gaatcgcgtc tggcacagtc gggcggaatg aacgcggtgg tgccgcagaa
     3961 cagcagcggc gttccggcac cgtcatcccc ggtggatgcg ccgcaggtac agaccatcac
     4021 ctccggacgt gacctgctcg accaggcgaa agtgctggcc cgttatctga acgaacagcc
     4081 gcagggctgg ctttcggcgc accggctgat gaaaaccctg cgctgggata cggtgcatga
     4141 actgccgccg gacgtggacg gcaaaacccg gctggcccca ccgcgcaccg agtcacgcaa
     4201 ccagcttaag cggctgtatg cccagcagaa ctggacggaa ctgctggagc aggccgacct
     4261 gatgttcagc tccggcgtca gccatttctg gctggatatt cagtggtatc tgcatcaggc
     4321 gctggctaag gcgggcgcac cgtgggacag atggacagcg gttatccgtc aggacctggc
     4381 gctgctgctg gagcgcctgc cggggctgga aaatctggca tggaacgacg gcacgccgtt
     4441 cgccgatgag gtgacgcgca actggatagc gcagcaggtc atgatgcggg aagacggcgc
     4501 atggcttgcg gggaaagccg ctgtaccgac agacgacgcc acaaacgacg tactggcgct
     4561 ggagccggaa gcactggaga tggcagacag tcagggcgtg gaagcggcgc tgggctggat
     4621 acagacccgt ccgggcatca ccacggcacg ccagcgcctg ttattgcggc tgctgatggc
     4681 gcgggtggcg gaacagtacg gtaaaaacga gatggcgctc ctgctgcttg aagaactgga
     4741 tattgccaca cagggaataa cgctgacgca gtgggagcca gagctgctgt ttgaggtaaa
     4801 agcccgacaa ctgaaactgc tgcgtctgcg ggcgcaccgc tatgcggata aagccctgct
     4861 gaaccgaaaa atggatgccc tgctgggtac actggtggca atcaatccgg tgcgggcggc
     4921 agtactgtgt gatacgcagc ataaggagtg atgacgatgg gaagaggtta ttttctggtc
     4981 aggggcgata agaccacctg cggcggcaaa attattgaag gggcggacga ccatacgatc
     5041 atgggaatcc cgcaggcgcg ggatatggac agaatgacct gtggcaagca tccggggatg
     5101 tttattatcg tcggcggcgt gccggaaacg ggtattcacg gcaggctgat ggcgggatcg
     5161 ctggacagcc agagcagttg cccgtgtaag gcgcggttta ttgcgtcgat gatggatgat
     5221 gtgtatgagg tggaaacaga aaaaactgag aacaagtctg ttatgccctt gaacccttta
     5281 aacaatggaa caggaaattc atcacctcct gcttatacct ctgattatcc tgcccttcgt
     5341 aatacgcaca atctgccaga ccagcaggtc agggatatgc tagcgaataa taatcatgat
     5401 gttatgttac tgacgtatga agagggcgct gaagttctgc aatcgtgggg ttgtcaggat
     5461 tttaagcgga aatgggttga catcacgcaa tctgcaccag ggcagttaat tattaattat
     5521 ggcgtaaatg gtaaagatgt agtgacaact tcaatcgtgc ttgcccgcct gggggatttt
     5581 ggtatcaaag caactgccta tgttaatcat aaaggaaccg aactgataaa acttacagga
     5641 tatcccggca ttcggaaaat actgaatgca ccggtgtttg cagctaaaaa ccccaaaata
     5701 gtagatgttg gaattgggaa atatggactc acaaactcta tagtaaaagg ggcaagatta
     5761 acgttttacg tcgcagctgc ttatagaact attgatttca tcctgaatga tgaaacatcg
     5821 ttggctgagt ttataggtgc gttggcaaca gatgttggta aaattgcgat agcttcagca
     5881 gttagttggg gcgcaggaaa ggcgttactg gccactgggt ttgttgctgg acctttggtt
     5941 ggggtagtgg ttgtggggat cggcgcggcc cttctattaa actatcttga taataaattt
     6001 ggtttaactg atcaagttgt cgcttatatt gaagcggctc agcaagaatt tgttgagaag
     6061 gcaagagaga ttgaagaagg gatttgggat ctgggagcca tgtgggcaaa acacatgctt
     6121 attaaaggga aggaagtact ggaacatgaa gtgaaggagt atttaaaaga taaactaaat
     6181 aatgtgagta aa
//
LOCUS       DAZFFE010000177         6156 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFFE010000177 DAZFFE010000000
VERSION     DAZFFE010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.178"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            54..143
                     /locus_tag="ACHJ69_004572"
     CDS             54..143
                     /locus_tag="ACHJ69_004572"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO5017398.1"
                     /translation="MTAYRNVFIQDDPGMHFRRVIRNAEGQRR"
     gene            322..1143
                     /locus_tag="ACHJ69_004573"
     CDS             322..1143
                     /locus_tag="ACHJ69_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5017399.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHHVPGIFGEDKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVF
                     DRIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            1198..1683
                     /locus_tag="ACHJ69_004574"
     CDS             1198..1683
                     /locus_tag="ACHJ69_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5017400.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            1699..2175
                     /gene="radC"
                     /locus_tag="ACHJ69_004575"
     CDS             1699..2175
                     /gene="radC"
                     /locus_tag="ACHJ69_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO5017401.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            2262..2483
                     /locus_tag="ACHJ69_004576"
     CDS             2262..2483
                     /locus_tag="ACHJ69_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO5017402.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            2646..3014
                     /locus_tag="ACHJ69_004577"
     CDS             2646..3014
                     /locus_tag="ACHJ69_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO5017403.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
     gene            3104..3478
                     /locus_tag="ACHJ69_004578"
     CDS             3104..3478
                     /locus_tag="ACHJ69_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO5017404.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            3475..3963
                     /locus_tag="ACHJ69_004579"
     CDS             3475..3963
                     /locus_tag="ACHJ69_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO5017405.1"
                     /translation="MKLSLMLEADRINVQALNMGRIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVITLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            3975..4172
                     /locus_tag="ACHJ69_004580"
     CDS             3975..4172
                     /locus_tag="ACHJ69_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO5017406.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            4257..4406
                     /locus_tag="ACHJ69_004581"
                     /pseudo
     CDS             4257..4406
                     /locus_tag="ACHJ69_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
     gene            4382..4510
                     /locus_tag="ACHJ69_004582"
                     /pseudo
     CDS             4382..4510
                     /locus_tag="ACHJ69_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            4848..6045
                     /locus_tag="ACHJ69_004583"
                     /pseudo
     CDS             4848..6045
                     /locus_tag="ACHJ69_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002441793.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1541 a         1550 c         1546 g         1519 t
ORIGIN      
        1 cagagtatgt cctggctgaa ggatctttct catacgggca ggcggttgct gtcataaccg
       61 cttaccgcaa tgtgtttatc caggatgacc cgggaatgca ttttcgtcgg gttatccgca
      121 acgcggaagg tcagcgacgc tgacgcagcc ggaactctga agctgatgcc ggaaaacagc
      181 ttaatgcgtg gctcgccagt ggagggcttc tcagacaata aacctcattg atttcatcca
      241 tcaggccgcg tcttctccgg gagacgcggc ttttttcatt tattacacca gtaaatctta
      301 accaccgata aggagcaaag tatgcgatta gccagtcgtt ttggttatgc tgcaaaccag
      361 atacgtcgtg accgcccact gacacatgaa gagctgatgc accatgtgcc ggggattttc
      421 ggggaagata aacacacgtc ccgaagccag aactatacgt acatccccac tatcaccgta
      481 ctggaaagcc tgcagcggga aggttttcag ccctttttcg cctgccagac ccgtgtgcgc
      541 gacccgggcc gccggggata cacaaaacac atgctgcgtc tgcggcgggc cggagagata
      601 aacggagaac atgtccctga aattattctg ctcaactctc atgacggtac ctccagctac
      661 cagatgctgc cgggttactt caggttcgtc tgccagaacg gatgcgtctg tggtcagtct
      721 ctgggggaag tgcgtgttcc gcaccgggga aatgtggtgg acagagtcat tgaaggggct
      781 tacgaggtgg tgggcgtgtt tgaccggatt gaggaaaagc gtgatgccat gcagtcgctg
      841 attctgccgc caccggcacg ccaggcgctg gcacaggcgg cactgactta ccgttatggt
      901 gacgaacatc agcccgtcac caccgccgac attctgacgc cacgacgccg ggaggattac
      961 ggtaaggacc tgtggagtgc ttatcagacc attcaggaga atatgctgaa aggcggaatt
     1021 tcaggtcgca gcgctaaagg aaaacgtatc catacccgtg ccattcacag cattgacact
     1081 gacattaagc tcaaccgcgc attgtgggtg atggcggaaa ccctgctgga gagcctgcgc
     1141 tgattcagcc attaccctga aaaccacgca tttatttgtc ctttaaggag tcatttcatg
     1201 aaaaccattt cacataattc caccacatct tctgtttccg taaccgctgc atcagggaat
     1261 gaccagcctc agttggttgc caccctcgtc cctgatgaac agcgcatcag cttctggccg
     1321 cagcactttg gcctcattcc acagtgggtg accctggagc cccgtgtctt cggctggatg
     1381 gaccgtctgt gtgaagacta ctgcggtggt atctggaatc tgtacaccct gaacaacggc
     1441 ggggcattta tggcacccga accggatgac gatgatgacg aaacatgggt actgttcaat
     1501 gccatgaacg gtaaccgcgc tgaaatgagt ccggaagctg ccggcattgc tgcctgtctg
     1561 atgacgtaca gtcatcatgc ctgtcgtacg gagtgttatg ccatgacggt ccattattac
     1621 cggctgcggg attacgccct gcagcatccg gaatgcagcg ccattatgcg catcatcgac
     1681 tgaatggaga gaagcacaat gcaacaactt tcctttctgc ccggagagat gacgcccggc
     1741 gagcgcagtc tcattctgcg ggccctgaaa actctggacc gccatcttca tgaacccggc
     1801 gtggccttca cctccacccg tgcggcacgg gaatggctga ttctgaacat ggcgggactg
     1861 gagcgtgaag aattccgggt gctgtatctg aataaccaga atcaactgat tgccggtgaa
     1921 accctcttca ccggcaccat caaccgcacg gaagtccatc cccgggaagt gattaaacgc
     1981 gccctgtacc acaatgccgc tgccgtggtg ctggcgcaca atcacccgtc cggtgaagtc
     2041 acacccagca aggcagaccg ccttatcacg gaacgtctgg tacaggcact ggccctggtt
     2101 gatatccggg taccggacca tctgatagtc ggtggcagcc aggttttctc ctttgccgaa
     2161 cacggtctgc tttaacccgt taccgtcaca atcaccttca tatcacttca gtttctcttt
     2221 ctcagctgtt tcttactttc acattcagga ggactattct catgaaaatc atcacccgtg
     2281 gtgaagccat gcgtattcac cgtcagcatc ctgcatcccg tctttttccg ttctgtaccg
     2341 gtaaataccg ctggcacggt agtgctgaag cgtataccgg tcgtgaagtg caggatattc
     2401 ccggtgtgct ggccgtgttt gcagaacgcc gtaaggacag ttttggtccg tatgtccggc
     2461 tgatgagcgt caccctgaac tgatgttccg tcacccggaa aacgagtacg actggctgag
     2521 ctcagacgcc gctgtggatg aggtcctgct catcaacgaa tacaccttta ccgaagcccg
     2581 tctgcgcgcc ggctgacccg tcatcgttaa actatcattc accgattttc tgtgaggatt
     2641 ttatcgtgtc agacacactt tccgggacaa cgcatcccga tgacaatgac gatcatccct
     2701 ggtggggact accctgcaca gtgacgccct gtttcggcgc ccgtctggtg caggagggta
     2761 accgcctgca ttaccttgcc gaccgcgccg gtatcagagg cctgttcagc gatgcagatg
     2821 cgtaccacct ggaccaggcc tttccgctac tgatgaaaca actggaactc atgctcacca
     2881 gcggtgaact gaatccccgc catcagcata ccgtcacgct gtatgcaaaa ggtctgacct
     2941 gcaaagctga cactctcggt agcggtggtt acgtttatct ggctgtttat ccgacgcccg
     3001 aaacgaaaaa gtaaaccacc ggaataacct tctgctccgg cctggtgctt tcaccacgcc
     3061 acttttccat ttttcatctc tgcatatcag gaaaaccttc agtatgaaaa cattacccga
     3121 cacacacgta cgggaggcat cgagctgccc gtctcccgtc accatctggc agactctgct
     3181 ctcccgacta ctgggccagc attacggcct tacactgaat gacacaccgt tcgccgatga
     3241 acgtgtgatt gagcagcata ttgaagctgg catttcactg tgtgatgcgg tgaattttct
     3301 cgtggaaaaa tacgcgctgg tgcgtaccga ccagccggga ttcagcactt gtccccgctc
     3361 tcagttaata aacagtattg atatcctccg ggctcgccgg gcgaccggcc tgatgacccg
     3421 cgacaattac agaacggtaa ataacattac cctgggtaag catccggaga aacgatgaaa
     3481 ctttccctga tgctggaagc cgacagaatt aatgtgcagg cactgaacat ggggcgaatt
     3541 gtcgttgacg tcgatggtgt taatctcact gaactgatta acaaggtcgc tgaaaacggt
     3601 tattcactcc gcgtggtgga ggaatccgac caacagtcaa cctgcacact accaccgttt
     3661 gcaacccttg ccggcatacg ctgcagtacc gcacatatca cggaaaagga taacgcctgg
     3721 ctgtactcgc tgtcgcatca gaccagtgac gttggtgaat cagaatggat tcatttcaca
     3781 ggtagcggat atctgttacg taccgatgcg tggtcatatc cggttctgcg gcttaaacgc
     3841 ctggggctgt caaaaacgtt ccgtcgtctg gttatcacac ttacccgacg ttatggcgtc
     3901 agtctcattc atctggatgc cagcgctgaa tgcctgccgg gtttacccac tttcaactgg
     3961 taaccaggaa caacatgaaa tcattaacca cggaaaccgc actggatatt ctgattgcgt
     4021 ggctgcagga caatatcgac tgcgaatcgg gaattatctt tgacaacaat gaggataaaa
     4081 cggattcagc agcactgttg ccctgtatcg aacaggccag agaggatatc cgtaccctgc
     4141 gccaactgca gcttcagcac cagaaccggt gagtcttact caccagactt cattccactg
     4201 acgccagcct gaacagggct ggcattttca tttatctgca aaaaggaata tcgattatgt
     4261 ctgatatcac agcctaccgt ccggaagtgg tcaacgagaa tacggacgtt atctgctcaa
     4321 ccagcatccg ccgcattctg gctgtacgaa agagttcact gctgcaaatc gacacaatta
     4381 tccggcatca agtgagcgat atcagttaac agatatagat ggagaagatg cttttatccg
     4441 gattggttct atcgcgatgg tgcgagtact acttgatgca atgaagcctg tcgaggaaga
     4501 tgacgagtaa cagagctcgg tatctcaatc aggagctgat tgtcctggtt cgatgccgaa
     4561 aaccagtgat ttttcgtgtt ggtatctgtg ttggtataag atgatattgt ttattattaa
     4621 caattttaaa tcatgaggtt aagtcacatg tttgagtccg gccttcgcac caatcaaggc
     4681 tttttcagtc tacttgtgta tataaatcaa taagttagat aatatccctc ttgtttcagc
     4741 tccattaaag tctactgtcg tatatcaata tctatcaata aatgttggta taactcttgg
     4801 tggcttctgt tcgatttata ttgatgccaa catagcgagg gggatgtatg gctcttactg
     4861 atatcaaagt cagaacggtt aagcctgcag acaaatcttt caaacttacc gatgatgagg
     4921 gggatgcacc tgctggttaa actaacccca atggcccaaa atactagcgt cttcaatacc
     4981 gtttaagtgg caaacagaag atgctggcgt tagggggtta tctatggcct cattggcaga
     5041 ggcttgtaag aggcacgatg cagcaaagaa actggtatct gatggcatcg atccctctca
     5101 aaagaaaaag aggaaaagat tgcagaaagc ggagcgctaa cctttgaggc tgtggtaaga
     5161 gattggcagg catcctgtag taaaaaatgg tcggagcccc atagtgaacg agtgcttaaa
     5221 atcttagtgg ataatctttt cctggcgtta agtaaacgta aaatttcgga actgaagaca
     5281 cggggtctac tcgtctttat aaagatagtt gaggtgagcg gaccttatga agttgtctaa
     5341 cgtcttcaac aaaatacaac tgctatcatg catttcgcgt tacaaaatgg tctgattgat
     5401 tataacccgg cccaagacat agctggtgcg gtagccgtgg ctaaacgagt ggatcgtcca
     5461 gggtttgatt aagaacgtct tcccgaatgg cttgatcgca tcgaatgctt taaaggtcga
     5521 aagtttacta aattggcagt gaaactcata ctgctggtat tcatacgttt aggtgaactg
     5581 cgttttacaa gatgggaaga aatcgatttt aaaaatgcac tttggacgat acctgcacaa
     5641 cgagaacctc tgaaggcgta aagcacgcac attgtggttc taagatgcgt agtccttatc
     5701 ttgtactatg aagccgtcag gcactggaaa tgttaggaga gataaaacaa accagtggtc
     5761 ttcataggcg ttcaccagcc ggttaggcag atgagtgaga acacggttaa taaggccctg
     5821 cgcttaatgg gctacgagat tacaacggaa gtttgtggac atggggttag ggcgatggcc
     5881 tgtagtgcgt tgatcgaatc aggtttatgg tcaggggatg ctgttgaacg tcaaatgagc
     5941 catcgggagc gtatcaatgt ccgaacttct tctatccatc ttgctgagca tcttgatgcc
     6001 aaccgagtca gaacggttag gtcgtttgag tttgcccaca gctgatcgtt ttgagtttga
     6061 aaatcaaagt taaccgcttg ataacctatc acataatagc atgtaactct ttgttttgaa
     6121 tatatttgag tagccatcaa ttgttgatgg ctacta
//
LOCUS       DAZFFE010000178         6154 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFFE010000178 DAZFFE010000000
VERSION     DAZFFE010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.179"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(9..1901)
                     /gene="aceF"
                     /locus_tag="ACHJ69_004584"
     CDS             complement(9..1901)
                     /gene="aceF"
                     /locus_tag="ACHJ69_004584"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO5017407.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMIFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(1916..4579)
                     /gene="aceE"
                     /locus_tag="ACHJ69_004585"
     CDS             complement(1916..4579)
                     /gene="aceE"
                     /locus_tag="ACHJ69_004585"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO5017408.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(4740..5504)
                     /gene="pdhR"
                     /locus_tag="ACHJ69_004586"
     CDS             complement(4740..5504)
                     /gene="pdhR"
                     /locus_tag="ACHJ69_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO5017409.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            complement(5960..>6154)
                     /locus_tag="ACHJ69_004587"
     CDS             complement(5960..>6154)
                     /locus_tag="ACHJ69_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFO5017410.1"
                     /translation="LKGKKLEKYLDTLVDHFIKITEHPKKGDLIFYPNSPEDGKPENI
                     LKIVKEWRRSQGLPLFKDSE"
BASE COUNT         1372 a         1644 c         1525 g         1613 t
ORIGIN      
        1 tcttttattt acatcaccag acggcgaatg tcagacagcg tgttgttaat gatggtaatg
       61 aaacgggcac catcagcacc gtcgatcacg cggtggtcga aggagagaga aatcggcagc
      121 atcagacgcg gcacgaactc tttaccattc cacaccggct ccatcgcgga cttggaaacg
      181 ccgaggatag ccacttccgg cgcgttcaca atcggcgcga agtgggtagt acccaggccg
      241 ccgatgctgg agatggtgaa gcaaccgccc tgcatttcgc ccgctgtcag cttaccgtca
      301 cgcgctttct tagaaatagt catcagctcg cgagacagct cgatgatgcc tttcttgttg
      361 acgtctttga ataccgggac aaccagaccg ttcggggtat ccaccgccac accgatgttg
      421 atgtatttct tcagggtcag acgctgaccg tcttccgaca gcgaactgtt gaagcgaggc
      481 atctgctcaa gagctgcagc aacggctttc atgatgaaga caaccggggt gatcttcaca
      541 tccagcttac gtttcgccgc ttcttcgttc tgctgtttac ggaacgcttc cagctcggtg
      601 atatcggttt tgtcgaagtg agtaacatgc gggatcatca cccagttacg gctcaggttc
      661 gcaccagaga ttttctggat gcggcccagt tccacttctt cgatttcacc aaacttgctg
      721 aagtccacct tcggccacgg cagcatgcct gggataccgc cgccagttgc cgccggagct
      781 gcttctgcac gtttgatagc ttctttcacg taagcctgaa cgtcttcgcg caggatacga
      841 cctttacggc cggtgccctt cactttcgcc aggttaacgc caaactcgcg tgccagacgg
      901 cggatcagcg gagtcgcgtg aacgtaagcg tcattttcag caaattcaga tttgccttcg
      961 gcttttgcag ccggagctgc tgccggggct tcagcttttg ctgccggagc cggcgctgcc
     1021 gcttcctgtt tcgcaggagc tgccgcaggc gctgcgcctt caacttcgaa gatcataatc
     1081 agcgaaccag ttttcacttt atcgccaacg ttgactttca gttccttcac gacgcctgca
     1141 aacggagccg gaacttccat agaagcttta tcgccttcta cggtgatcag tgactgttca
     1201 gcggcaactt tgtcgcccac tttcaccatc acttcggtca cttcaacttc gtcaccgccg
     1261 atatccggaa cgttaacttc tttcacgcca gctgctggtg caggggccgc tgccggagcc
     1321 gcttcctgtt tagccgccgg agctgccgcg cctgcttcac ccgcgacttc gaagatcata
     1381 atcagcgagc cggtagacac tttgtcaccc acgttcactt tgatctcttt cacggtgcct
     1441 gcaaacggag caggaacttc catagaagcc ttgtcgcctt ctacggtgat cagcgactgt
     1501 tcagcttcaa ctttatcgcc aactttcacc aggatttcgg tcacttcaac ttcgtcgctg
     1561 ccgatatccg gaacgttaac gtcttttgct gccgcagccg ctggtgctgc tgccggagct
     1621 gcttctttct tctcttctgc ctgagcaggt gcagcgtctg ctgcaccgtc ggcggaatcg
     1681 aaaatcataa tcagtgcgcc ggtctgggtt ttatcgccaa cagagacttt gatctcttta
     1741 acgatacccg cctgcggaga tggtacttcc atagaggctt tgtcgccttc tacggtgatc
     1801 agcgactgtt cggcttcaac tttgtcgccc actttgacca ggatctcggt gatttcaact
     1861 tcatcagccc cgatgtccgg tactttgatt tcgatagcca ttattctttt acctcttacg
     1921 ccagacgcgg gttaacttta tctgcatcga tgttgaattt ggcgattgcg tcagcaacca
     1981 ctttcttatc gatttcgcca cgtttagcca gttcgcccag cgccgcaacc acgacataag
     2041 aagcatcaac ttcaaagtgg tgacgcaggt tctcacggct gtcggaacga ccgaagccat
     2101 cagtacccag tacgcggtag tcgtcagccg gtacgtaagt acggacctgc tcagcgaaca
     2161 gtttcatata gtcggtagat gccactgccg gagcgtcgtt catcacctga gcgatatacg
     2221 gaacgcgcgg agtttccagc gggtgcagca tgttccagcg ttcacaatcc tgaccatcac
     2281 gcgccagttc ggtgaaggaa gtcacgctat aaacgtcaga acctacgccg taatctttcg
     2341 ccaggatctc tgccgcttca cggacgtgac gcaggataga accggagccc agcagctgaa
     2401 ctttaccttt gctaccttca atggtttcga gtttgtagat acctttacgg ataccttcct
     2461 cagcaccttc cggcattgcc ggcatgtggt agttttcgtt cagcgtagtg atgtagtagt
     2521 aaacgttctc ttgtttttca ccatacatac gctccagacc atcatgcatg atgacagcaa
     2581 cttcgtaagc gtaagccggg tcgtaagaga tacagttcgg gatagtcagc gactgaatat
     2641 ggctgtgacc atcttcgtgc tgcagacctt cgccgttcag ggtggtacga ccggaagtac
     2701 cgccgatcag gaagccacgc gcttgctggt cgccagccgc ccagcacaga tcgccgatac
     2761 gctggaaacc gaacatcgag taatagatgt agaacgggat catcggcaga ttgttggtgc
     2821 tgtaagaggt cgccgctgcc agccaggaac aacctgcgcc cagctcgttg atcccttcct
     2881 gcagaatctg acctttctcg tcttctttat agtaagcaac ctgctcgcgg tcctgcgggg
     2941 tgtactgctg accgttcggg ctgtaaatac caatctgacg gaacagacct tccataccga
     3001 aagtacgcgc ttcgtcggcg atgatcggta ccagcctgtc tttgatcgac ttgttcttca
     3061 gcattacgtt cagggcacga acgaaagcga tagtggtaga gatctctttg ctctgctctt
     3121 ccagcagcgc gccgaagtct tgcaggctcg gcagctcaag cttctcggtg aagttcggct
     3181 gacggcttgg cagataaccg tgcagtttct gacgctgagc gtgcagatag gtatgctctt
     3241 cagaaccttc cgggaaggtg atgtacggca gtttttcgat atctgcatca gacaccggca
     3301 cattgaaacg gtcgcggata tgacgcacgc cgtccatgtt cattttctta acctggtgcg
     3361 cgatgttttt accttcagcc gcgtcgccca tgccataacc tttaatggta tgagcaagga
     3421 ttaccgtcgc tttgcctttg gtttcctgcg ctttcttgaa tgcagcgtag attttcttcg
     3481 gatcgtgacc accacggttc agtgcccaga tctgctcgtc agtccagtct gcaaccagtg
     3541 ctgcggtttc aggatattta ccgaagaagt gttcgcgaac atacgcacca tctttcgatt
     3601 tgaaggtctg gtaatcgccg tcaacggttt cgttcatcag ctggatcagt ttaccgctgg
     3661 tatctttacg cagcagttcg tcccaacggc taccccacat cactttgatc acgttccagc
     3721 cagcaccttc gaagatgcct tccagttcgt tgatgatctt gccgttaccg gtgaccgggc
     3781 cgtcaagacg ctgcaggtta cagttgataa cgaagaccag attatccagt ttttcacggg
     3841 tagcgatggt gatcgcacct ttggattccg gttcgtccat ttcaccgtcg ccgaggaacg
     3901 cgtaaacggt ttgtttagag gtatctttca ggccacggtg ttccagatat ttcaggaatt
     3961 tagcctggta aatagcacca atcggaccca gccccataga tacggtcggg aactgccaga
     4021 attccggcat cagtttcggg tgcggatagg aagagaggcc attgccgtga acttcctgac
     4081 ggaagttatc cagctgctcc tgagtcagac gaccttccag gaaagcacga gcgtaaacgc
     4141 ctggggagat gtggccctgg aagtaaacca ggtcgccgcc atcctgctcg ttgcgtgcac
     4201 ggaagaagtg gttaaagcac acatcataaa tggttgcgga agactggaag gacgccatgt
     4261 ggccgcccag ttcgaggtct tttttcgacg cacgcagcac cgtcatgatg gcgttccagc
     4321 ggatagctga acgaatacgg cgttccagtt ccagattacc cggatactcc ggttgttctt
     4381 caacggggat ggtgttgatg tagttgctga taccggtgcc tgcagctacg ttgactccgc
     4441 ctttgcgggc ttcagcaagc agttggtcga tcagatactg agcacgctca acaccttctt
     4501 cacggatgac cgattcgatc gcctggagcc agtcgcgagt ttcgatcgga tccacgtcat
     4561 ttgggaaacg ttctgacatg ggttattcct tatctatcta ataacgttga gttttctgga
     4621 acctgtccca ttgaactctc gccggaaagc tcaataagac aggttctacg tttagttgcc
     4681 gcgcttttat atgcgcttga tttacaacat cttctggata atttttacca gaaaaatcac
     4741 taattctttc gttgctccag acgacgcaga gaacgctcac ggcggctctc ttcacggctt
     4801 ctgtcgagca aaatttcttc gataaaggcc agatggcgat gcgatgcttc gcgcgcttct
     4861 tccggcttac cggccataat cgcttcaaat atgcgggtgc ggtgactact caccagcggc
     4921 agcatctcac gacgcgaata gagcaattcg aagttctggc ggacattctg ggccaacatc
     4981 ggctccatac accttagcag atgaagcaga accacattgt gggccgcttc ggtgacggca
     5041 atctgatact ggagtacggc gtttgattcc gcgtccagat cgccagactg ctgcgccagc
     5101 tctatggcat ggtggagttc acggatgcgt tccttgtctt catcggtact acgcagcgcg
     5161 gcgtaataag cggcgatacc ttccagggcg tgtcgtgttt cgagcaagtc atactgtgat
     5221 tcaggatggt cggagagcag ctccaccagc ggatcgctga agctttgcca taggctgctc
     5281 tggacaaaag tgccgccacc ctggcgacga agcaacaagc ccttcgcttc gagacgttga
     5341 atcgcctcac gcaatgaggg acgggagacg tcaaactgtt ttgccagttc gcgttccggt
     5401 gggagttttt cgcccgggcg gagagtgcct tcgaggatca aaaactccag ttgctgctca
     5461 atcacatcgg agagttttgg ttggcggatt ttgctgtagg ccatgagttc ctgtcttaag
     5521 ccacttgccg aagtcaattg gtcttaccaa tttcatgtct gtgacgctaa agtaacaaag
     5581 tattcacctt atgtccatac aggttttgat tgaaatcatg aaactgtgca cattttaaca
     5641 acttgacata tataacgttt caaagttgta actatgcaca aatgttaaat ttacccatca
     5701 aggggtgacc ttaacagaat tgaaacgaaa aaatatttcc tctgaaccga ttcaccaagc
     5761 aacaaaacgc ataacaattc aattatgtga attaatatgt aaaatgaatg ttcagggaag
     5821 gagagaaagg tagtatttac ctatgttttc tttttacatt gcttattatc actgcaatcg
     5881 ccaccgtaac cttctcgtag cgaaaatcat cacttaacca gaacgtgatg attttcaggt
     5941 gacagattct gtttagagtt cattctgaat ctttgaacaa aggcaatcct tgcgaacgcc
     6001 gccattcttt gacgattttc aggatgtttt caggtttacc atcttctggg gaattaggat
     6061 agaaaatgag atctcccttt ttaggatgtt cagtaatttt aatgaaatga tccactaagg
     6121 tatcgagata tttttctaat tttttccctt ttag
//
LOCUS       DAZFFE010000179         6153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZFFE010000179 DAZFFE010000000
VERSION     DAZFFE010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.180"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..773)
                     /locus_tag="ACHJ69_004588"
                     /pseudo
     CDS             complement(1..773)
                     /locus_tag="ACHJ69_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    complement(376..492)
                     /locus_tag="ACHJ69_004588"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            848..1001
                     /locus_tag="ACHJ69_004589"
                     /pseudo
     CDS             848..1001
                     /locus_tag="ACHJ69_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011149570.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(1100..1534)
                     /gene="psiB"
                     /locus_tag="ACHJ69_004590"
     CDS             complement(1100..1534)
                     /gene="psiB"
                     /locus_tag="ACHJ69_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO5017411.1"
                     /translation="MNTELNLNVLQTMNALEYEDIRAAGSDERRELTYAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWMLVLVNSGGEPFSMV
                     QVHRLFTPVAVSHSLALAASLDERGYSVSDIIHILMAEGGQS"
     gene            complement(2320..2547)
                     /locus_tag="ACHJ69_004591"
                     /pseudo
     CDS             complement(2320..2547)
                     /locus_tag="ACHJ69_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2620..3180)
                     /gene="istB"
                     /locus_tag="ACHJ69_004592"
                     /pseudo
     CDS             complement(2620..3180)
                     /gene="istB"
                     /locus_tag="ACHJ69_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
     gene            complement(3283..3435)
                     /locus_tag="ACHJ69_004593"
                     /pseudo
     CDS             complement(3283..3435)
                     /locus_tag="ACHJ69_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3632..3835)
                     /locus_tag="ACHJ69_004594"
     CDS             complement(3632..3835)
                     /locus_tag="ACHJ69_004594"
                     /inference="COORDINATES: protein motif:HMM:NF020355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO5017412.1"
                     /translation="MRIITIPLLCRYASSPVRSRCSVTRLRRAFQTAAVASEQISVFP
                     VINDISLKDYQLLLKVAEEVDKK"
     gene            4212..4913
                     /locus_tag="ACHJ69_004595"
     CDS             4212..4913
                     /locus_tag="ACHJ69_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO5017413.1"
                     /translation="MTMTISTAKKTVNISQSNELTEAAYYLPLQAKRVLWLCLIQCYP
                     QKDDPDSVSPVFTVLVADYQKYFKVSVDTASADVKKGVTALAESSVVFYPKDCEFDEI
                     KRPWLAEAGLKKGRGKWQIEFNYKVMPYLMGLSSQFTTYSLYDCGKINSVRVIRLYES
                     LCQYRSSGVWVTTQEWLSERFMLPASQRSNFAEMKRTFINPALKKINTNTPLKATMIQ
                     NENGSLVFTIVDSKK"
     gene            5137..6153
                     /locus_tag="ACHJ69_004596"
                     /pseudo
     CDS             5137..6153
                     /locus_tag="ACHJ69_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    5418..5534
                     /locus_tag="ACHJ69_004596"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         1514 a         1547 c         1444 g         1648 t
ORIGIN      
        1 gcatccatgc ccgccctgaa ctccgggcac accgtaagta aaatcagcca cccacaactg
       61 gtcaggacgt tctgccacga actgacggtt tacgcggtcg cctgcggcaa cggctttccg
      121 gctgatggtc gtacggacct ttttaccccg gagaacaccg gcaagtccca taaccgccat
      181 gagacgtgcc acagtgcacc tggccactct gataccttcc cgtaacaact gacgccagac
      241 tttacgcaca ccgtacacct gatgattttc atcgtatacg cgctgtatct ctttcttcag
      301 ccagtcatcg cgctgcgcac gggcactgcg tttatccgga tgatgtcgct gttgctgaca
      361 gtggtaatac gttgacgggg caatatgtag ttcactgcat accggtccga ccccgtacag
      421 cttacgcagc ttatccagca gtggtatcat tttttccaga ggcggtcgaa ctccgccttc
      481 gcaaaataag cggaagcctg gcgaaggata tcgttactgc ggcgcagttc aagattttca
      541 cgttccagtt ctttcagacg ctgacgttca gtggtggtga gcccgccatc accgcctccg
      601 gtatcccgct catgctggcg tacccagaca cgcagagtct ccggtgtaca gccaatcttt
      661 ggggcaatgg aacaaattgc cgcccactgt gagtcatatt cgccctgact ttccagaacc
      721 atacgaatcg ccctctgacg gacttcgggg gaaaaacgag tatttttagt catcctgttt
      781 acctctttct cagggagttt agtctccagg attcccgggg cggttcatag tgctgggaaa
      841 cacggcatca ccgggaacca tcccgaaacg accggagcgt ctgcgggatg ctctggaagt
      901 ggtgttcata acaaaacgcc tccgaccatc aaggccgagg gcggttaaga tatcaaaaaa
      961 accgttatcc tgtgaaacgc agtgccgctc cgcttaagtg aacggtattg cgcacggagc
     1021 ggcctttttc cgtctcagtc atacccacca tgcggtctgt tgctctgtgc agaggggggc
     1081 aatgtctgtg aacgtgctct catgactgac ctccttccgc catcaggata tggatgatgt
     1141 cactgacgga atacccccgt tcatccagtg acgccgcaag agccagtgag tgactgacag
     1201 ctaccggtgt aaacagacga tgcacctgaa ccatcgaaaa cggctcacca ccggaattca
     1261 ccagaaccag catccagacc tgagagacat cgcccggcga acataacgcc aggtgaaaac
     1321 gttcgtgggc aggtgtgaaa cggacctgga cggggaaaaa gccgccaaac tcactgccat
     1381 actctccgtt catcgtccag ttgtccggcg catccagctc ccgcatcacg gcgtatgtca
     1441 gctcacggcg ttcatcactt ccggcagccc ggatatcttc atattccagt gcgttcatag
     1501 tctgcaggac attcaggttc agttcggtgt tcattgtgat atatattcct tattggcgcc
     1561 ccgtctccgg cggggcggtg tgattgttca tcaagtcggc atcagccgga ttgtctgtgt
     1621 attcactggg gttagtctct gtctgtggac gcgtagtgca ctgcatattt agctgtatca
     1681 cctttcttca tatcctgtgc ggcactggat acaaccactt caatttcggt cacctagtga
     1741 cgaatgctct gtacttctag tttgtaactc catcctgaca ggccacttca aacacataaa
     1801 gatgacgctc catgtcgttc tccggcgata gagttcagtt gtgattctgt cttctgccag
     1861 cacgtcgcga atttgctcgg atattttctt tactgcgtta ttgcacgaac tccaacaatc
     1921 tacttcgtag gagtcgtatt ccgatggtga ccgtttttaa ttcctgcgga attgcttttt
     1981 aagcgtacag acctgtcagc tactctaggg cagacggcac gattttctac attttgcaga
     2041 tttagggagg atttggtaca gtctcttcta aagacatgac tatctcctca tcgtgcagtt
     2101 aatgtctgtc ggacgcttcc ccgtccgaca ttgtgcactg acagtttcag catgcttccg
     2161 gaataattct gaatattcgc tcattgtcac ccaatgatgc ctcgtatatg acggggaggc
     2221 gatcctgtca gcatagtgcc tatagtttta acaatgattt cagaaaaata acgggagtgc
     2281 tgtttataga tgggtcttcc ctgatcatgg tgggaaggct cagaacgcca tattcagttt
     2341 cccgtagtgg aacatcacgc ccagcttaaa gcgctcccga ttgcggtatc ccttggcttt
     2401 tatcctcagt agcctgatct tactgttaag cgcctccgcg tttccgtttg aggcacggtg
     2461 tcgcatggca ttcaggatcc cgtaaagcct ttttcctatc gttttcgcgg cgtttttcat
     2521 cataggaacg tcactgttgg ccgccagaac ctgtcatctt gtcaacgcca cgatgtttga
     2581 ctgttatttg ccattttcat gcccgctaag atactttcgt tactgatgct gtccgctttt
     2641 caccatttca tcatcactga ttggcgttgt gtttttggtc agcactccag ctttacgttt
     2701 ctcttttaac cggtaactct ctcctttgat attcagcgtg gttgagtgat gtagcaaccg
     2761 atccagtatc gctgttgcca gcacgtgatc tccgaacatt tctccccagt ctgcgaaccc
     2821 tttgtttgac gtcagtatga tgctcgcttt ttcatatcga cggttcagta gccggaagaa
     2881 caggctggct tcctctctgt tcatcggcag atagcctatt tcatccagga tcaacacccg
     2941 ggcataactc agttgctgca gctgacgctc cagccggttt tcctgtttcg ctttcatcag
     3001 tgtcgcgatc agtctgtcca atggcataaa cagtacccga tgtcccgcat ccaccgcttt
     3061 cacgccaaga gctatggcca gatgcgtttt tccgacacta ggaggtccca gcaggatcac
     3121 gttttcgctg cgctccacga acgccagacc agccagttcc cgaacaatct tacggtcgat
     3181 gtcctttctg gtgtgagaac cttaaggaaa caacatgttg ggtggagcgg acaatccaaa
     3241 tggtgaatta ccgtcttata tcactggcgc tgacaatctc tcccagtcat tacgtctttc
     3301 ctcactccat ggcctgttcc agatatcctt tgccagctct ttcagcgccc agcactgtct
     3361 tgtcagcttc atctgtgcac acagccatat cagcttttcc tacctggatt cggtcatcca
     3421 tttatcgctg tactgtctgc ttgacacgtc agttttcaga catggacact tgtaacggtg
     3481 cgacagcatt cgaatatagt acagccacaa ggttgcagtg cgtagaggaa aaacaacaag
     3541 actatactta gttaatatat cagcatggct tggctaactt ggcatttgct cactgatttc
     3601 aaattttttc catcaattct ttcaaagaca cttatttctt atctacttct tcggcaactt
     3661 tcagtaataa ttggtaatcc tttaatgaga tatcattgat gacaggaaat actgaaatct
     3721 gttcacttgc tacagcagct gtctgaaacg ctcgccgcag ccttgtgacc gagcatcgcg
     3781 agcgaacagg cgaggaagcg taacggcaca atagtggaat agtgattata cgcatctgat
     3841 tcaaaagata ttatgggaaa taacctaagt ttaatgacaa ttcaggtcaa cttatccact
     3901 tatccactta tccacaaact atttattaaa cattttatat ttttttactt tttatccttt
     3961 ttaggagcct ctgagtccct gtcgcacttg tatttcagat ttccttctat ggggatgacc
     4021 tcaagttact atgggaaatg gatatagtta ttatgggtaa tacaacaagt tactatggga
     4081 aatagcacca gttcctgtgg gaaatgcccc tagtttaaat gcatatgcta taactgtgga
     4141 taactcatta atttcgcttg ttaggtttac atctgcatga caatatagga attaaaaaaa
     4201 cctatatact aatgacaatg acaataagta ccgcgaaaaa aacagttaac attagtcaat
     4261 ccaatgaact gacggaagcg gcatattatt tgccgttgca agccaagaga gtattatggc
     4321 tatgtcttat ccagtgctat ccacagaaag atgatcccga ttcggtatct ccggtattca
     4381 cagttcttgt tgctgattat caaaagtatt tcaaagtgtc tgttgataca gcgtcagccg
     4441 atgttaaaaa aggcgttaca gcactggctg aaagcagtgt tgtcttttat ccaaaggatt
     4501 gtgagtttga cgagattaag cgtccctggc tggccgaagc tggtctaaaa aagggacgag
     4561 ggaaatggca aattgaattt aactacaaag taatgcctta tttgatggga ttgtccagtc
     4621 agtttacaac atactcactc tatgattgcg ggaaaatcaa cagtgtcagg gtcatcagat
     4681 tgtacgaaag cttatgtcaa taccgttcca gcggagtatg ggttacaacc caggaatggc
     4741 tgtcagaacg ctttatgctt cctgcatcac agagatcaaa cttcgctgaa atgaagcgaa
     4801 cgttcatcaa tccggcactc aaaaagataa atacaaatac accactcaaa gcgacaatga
     4861 tacagaatga aaatggtagc ttggtcttca ccattgttga ttccaaaaag taaccaaact
     4921 gtatacaata cgacgaaggt aaactagggc tatttcccat agtgaaataa tctgtattag
     4981 ctcagacctg atctgacagt taccggttat ttatacaggt atctgtcaga ttacatctgg
     5041 cttaaatttt tctcgaccca gatgcgcttt ccatcaagta actgaaccgc cccgggaatc
     5101 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac tcgtttttcc
     5161 cccgaagtcc gtcagagggc gattcgtatg gttctggaaa gtcagagcga atatgactca
     5221 caatgggcga caatttgttc cattgctcca aagattggct gtacgccgga gactctgcgt
     5281 gtctgggttc gccagcatga gcgggatacc ggaggcggtg atggagggct caccaccgct
     5341 gaacgtcagc gtctgaaaga gctggaacgt gaaaatcttg aactgcgccg cagtaacgat
     5401 atccttcgcc aggcttccgc ttattttgcg aaggcggagt tcgaccgcct ctggaaaaaa
     5461 tgatgccact gctggataag ctgcgtgagc agtacggggt cggaccgcta tgcagcgaac
     5521 tgcatattgc cccgtcaacg tattaccact gtcagcaaca gcgacattat ccggataaac
     5581 gcagtgcccg tgcgcagcgc gatgactggc tgaagaaaga gatacagcgc gtatacgatg
     5641 aaaatcacaa ggtatacggt gtgcgtaaag tctggcgtca gttgttacgg gaaggtatca
     5701 gagtggccag atgcactgtg gcacgtctca tggcggttat gggacttgcc ggtgttctcc
     5761 ggggtaaaaa ggtccgtacg accatcagcc ggaaagccgt tgccgcaggc gaccgcgtaa
     5821 accgtcagtt cgtggcagaa cgacctgacc agctgtgggt ggctgatttt acttacgtca
     5881 gcacatggcg gggcttcgtc tatgtggcgt tcatcattga tgtgtttgcc ggatacatcg
     5941 tggggtggcg ggtctcatcg tccatggaaa cgacattcgt gctggatgca ctggagcagg
     6001 cgttatgggc ccgtcgaccg tccggcacgg tccatcacag tgataaaggt tctcagtatg
     6061 tatcgctggc ctacacacag cgacttaagg aagccggatt actggcatca acaggaagta
     6121 caggcgactc gtatgacaac gcgatggcgg aga
//
LOCUS       DAZFFE010000180         6069 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZFFE010000180 DAZFFE010000000
VERSION     DAZFFE010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.181"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(74..2365)
                     /gene="opgB"
                     /locus_tag="ACHJ69_004597"
     CDS             complement(74..2365)
                     /gene="opgB"
                     /locus_tag="ACHJ69_004597"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO5017414.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLAAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSRTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKAIWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(2619..3113)
                     /gene="yjjA"
                     /locus_tag="ACHJ69_004598"
     CDS             complement(2619..3113)
                     /gene="yjjA"
                     /locus_tag="ACHJ69_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO5017415.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNN
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNVAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            complement(3162..3899)
                     /gene="dnaC"
                     /locus_tag="ACHJ69_004599"
     CDS             complement(3162..3899)
                     /gene="dnaC"
                     /locus_tag="ACHJ69_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO5017416.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            complement(3902..4441)
                     /gene="dnaT"
                     /locus_tag="ACHJ69_004600"
     CDS             complement(3902..4441)
                     /gene="dnaT"
                     /locus_tag="ACHJ69_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO5017417.1"
                     /translation="MSSRVLTPDVVAIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTAEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            complement(4549..5022)
                     /locus_tag="ACHJ69_004601"
     CDS             complement(4549..5022)
                     /locus_tag="ACHJ69_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO5017418.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            complement(5013..5783)
                     /locus_tag="ACHJ69_004602"
     CDS             complement(5013..5783)
                     /locus_tag="ACHJ69_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO5017419.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFLASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
BASE COUNT         1465 a         1532 c         1545 g         1527 t
ORIGIN      
        1 ttctcaataa tgttttgtag gccggagaag gtgtgtaggt cgcaccttat gcgacctaca
       61 catcagtcag gaattaccct tcacgttcta ccactttaat ttccaccatg ccgatcccga
      121 gcttacgcgg cgagtgcccg aggatattcc cttcgttggt tgagacaggt tccggtggca
      181 caattaccag tgtgtcggca tcggttgggt tatcgaaatg cagtgtggtg gtggtcactt
      241 cattgcccag cacaagggtt tgttcttcat tgcctacgcg taccggaata ggacggctgg
      301 cgttattgcc gtatgctttg gcggtaatca ccaggtcaaa tttcttcggc agcggatgct
      361 tgtactcgat ttttacttca tcgcccagct gtgcgttgga ccagcggccc cacgactctg
      421 gacgagaaat cccgctaaac tgtttcacct cttctggcgc accagcgacg ttaaagatga
      481 aactgtcggc tttgtagcga atatcgttat caacaatctt tagcgtatcg acattgcctt
      541 tgtaacgcgc catgtcgatg accgtatctt taaatgccgt ttttcccttc catattgctt
      601 tgtcaacatg ctgaacaatt tgctgaccgc caagctgccc ttgcgagaca caccagtcgg
      661 tggagagtgc cagttccgga gcccagagtt gtgccatctt gtagcaacgg tcaacccaga
      721 cgaaattgtc gcgtggagcg aaatctgcca gctggaaacg cagcggtgct gagtattcgc
      781 tttccggcag cggttccacg cgtttgtctg aaacccgcaa cagcagcggc aaacggaaat
      841 ggctacctga gaaggcaatc atgtttttct gctggtcgat ggtgaactct ttcatctctt
      901 tagggaattt ccacaggcgg atgatatccg gcttccacgc caatgttttc tctttgatat
      961 tgaggaagat ttccgacatc gactgcccgg ataaactgct acggccaagt ccgagatagt
     1021 tatcgccacc gagaatgtcc agcaccgtcg caccgttatc catcgtatta cgcttcactg
     1081 ccagcgtctc ttgctgcggc ttgtcgccac ggatgacaaa aaacaggtta ttgcgatcct
     1141 gcttattaag gtatttccac gccgtattgt tcatcgctaa atggtcagaa gagacgacga
     1201 tgacggtatc tttaaaccac ggtgacgctt tgattttgtt gataaacgtc gcaatattct
     1261 cctggctgca acttaccgcg ctgaatgact gattcggctt accgtcaaaa tcatattttt
     1321 tgcggttaca ggtgcgcgag ataaaaccat ccgggtgatg ggtatcgact gtcagggtaa
     1381 acagtgagaa tcgctgacct gagcgggaaa gctcttcaaa ttttttccac gcttcatcga
     1441 gaacggtatc gtcgtagaat ccccagtcgt tgcgatagtg cgggtcggcc accacgcttt
     1501 tcagctcttc tgagccgtat aagtggtcga agccgtgcga tttcaggaac acatctttac
     1561 cggcaaaacg cagattcgcg ccctgcacga aatagttctg ataacccgag tttttcagaa
     1621 tatcgcccag acagatgttc tgcgggaaga agctggagac agaggcggag gcgttgcctt
     1681 caaatggggc aaacagcggt atgccgcact gagaagctac catgcccgca atcgtgtaat
     1741 ccgttcccgg cagctgctgc gtgtggctga aatccaggcc ttcatttttc aacgcgccca
     1801 gttcaggcgt gagatccggg aaagcctcgt tatcaaaata ggtccgctcg agactttcgc
     1861 cgtagatata aaccaggttg agtttcgggt cagggatagt tctcgacggc tctttataat
     1921 aagccgcaaa gtccgggtcg ccgtcgcgtg actgggattt caccagttcc gttatctgac
     1981 gaaatgccgg gctggcatcc actgaaccca gcgccagtaa gagtgccagc aggctgtaac
     2041 caaaatggtg cggatgatgg cgacgacggc gcaggatcca gcccagcgca ccgaacaccg
     2101 ccgccagccc cagcacaatg ccgataccag gcaggatgta tttgctgaca ccagcaccgg
     2161 tcaggctgtt ggttaaggta tagagaaccg cgtcgttaat accatcgcca gtaaaatagt
     2221 cgctggcaaa cagggtgata tttaaaacga caaatagccc cagtaccgtt aatgtggccg
     2281 caaaccacca ggtgttacgt cccgctttcc atgcgtaaat cagcacagag gcgagaaaaa
     2341 gggcgaaaga gagtagttct gacaacgggc gatcctcact aaaccgggcc gtttagccaa
     2401 cgtttaagct ttttgagaag cacaatgtaa tggcgtcgcc atattgctgc aattctagtg
     2461 tcaaaaaaat gcgatgttgt taggaattag tttataaaca gacttttttg atcgcggtcg
     2521 cttcccgtgt aacaggggta aaaccgtcca ggagcaggga aaagggcagg agagagccgc
     2581 gtcagccggg taacagctaa aacgcggcgt ttcgcggttc aggaaatgaa gttcagcccc
     2641 tgctttaaca ccagatcgca ggctttggtt ttcacctttt ctgccagcgg agtcgttcca
     2701 atattatcca gattgagttg ctgaccatct ttggttttca gcaaaccctg aatgccatcc
     2761 agatagttgg tgtcttcttt ttgctcttca ctgttcaggc ccagtttttc cagtacctga
     2821 ttcttgatgt tttcggcatc ggttaccgaa gccagctttt gcttcgcgca gtattgcaga
     2881 atgcctgcga cgttgttcat gttatctgcg cttaaggcct ggttcccgct gctaagcaag
     2941 ttagttaatg acgcgagcga ccaaccgcct tcctgtgtcg tgttgttttg gttgccaagt
     3001 tcgctggcgg cgctggagag cgcatctttc caggacgcag catgcacccc ggtggaaatt
     3061 aatgcgctgg cggcaatggc acagcacaga agatgtttaa tagttttcat catcattact
     3121 caaggttgaa ttgtgtcgca gtataccgcc agaaacgggg attaatactc tttacctgtt
     3181 acacggctac ggtagctatc ccagttgaag atcacccaca aactgttacc caggcgcata
     3241 cggtccatca cgcgttcgcc cagcaacttg gtcatctctt ccatattact gttggtcagc
     3301 atcccggttg ggcgtttgga agaagagcgg cgatcgacga tctggttgat gatcactttt
     3361 tcgtatttcg attcggtctg cacgccgatc tcatcgatca ccagcagatc aacgttactc
     3421 agatcgttaa gcagttgttc ttcgctggta ccgctattcc tgaaggtatc tttcatcgcc
     3481 gacataatat cggccacggt gatgatcaat acggatttac cgcgtagcag cagctcgttg
     3541 cagattgccg ccgccagatg gtttttgccg gttcctggct taccagaaaa gatgaagctg
     3601 gcgatgttgc cgtcaaactc ttcgacatac tggcgcgctt tgcttaacgc attcatttgc
     3661 ccttcacact caacgcgata gttctcaaag gagcagttct gatgcagtgg acgaataccg
     3721 gagcggttaa aggtgcgctg cattttcatc gcccgatttt cacgttcgag agcggcggag
     3781 cggatcgccc cttgttcttt ctgccaggcc agtagttctt cacccgtttt gaaagcaggt
     3841 ttgatatggg caggcatcat tttttgcagg cgttgcatca agtcgccaac gtttttcatc
     3901 gttaccctct gaatcctggt ggaatttggc tgtcaggttc actgaccgta ttcacatctc
     3961 gtttcggcag tccgccgttg ctggcacgac cgatttgcag gctgcgcgcc agtttttgtt
     4021 gccactgcac atggtgaaag actttgcctt ccgcctgcca gtaggcaatg aatgacgcca
     4081 gttcttcggc ggtcaccggc tctcttagtg ccacgcccca tagcgccgcc aggcggataa
     4141 agtcggcatc gggttgccag tccgggtaca tggcaaattt ccccattggt acagcaacgg
     4201 gagcggcttg cggttcctga tagagttgat cgtccagagc gacatcgctt cccggacgcg
     4261 ccagcttttc ttccagcgcc agcagttcag ccaggcgtgc aggcgtgacg gcataaaacg
     4321 ccggggcatt gttagcaaat acggcaacca caccgccttc agcttttgcc agaacggttt
     4381 ggtgatcgtg taccagggcg tcaatagcaa cgacgtccgg ggtcaaaact ctggaagaca
     4441 tactgtttct caaatatgga acgatgaacg agaagggaga taacttttat agtaacacac
     4501 ggaggagagg aggctgtctc tcatccgcgt gctttaagtg aggcaatttt atacgcgagg
     4561 gcgtttgcgg tacaaccata atccaggaat ggaaagaccg atggataacg caccaacaat
     4621 cgatgaagct gtaagaaagt tggttaacag ggtaatcatc aacggttcgc tgtaacctag
     4681 ctggctgatt tttaccgccg aaatcattgc ggtataagct gatatgccag ggaacatagg
     4741 gataacagcc gccacggtaa acactttcgg atgcgccaga taccagcgcg accattgaat
     4801 accaatggtg ccgaccagca tagaggccat aaaggttgac cattcaatat tcagcccgct
     4861 ggtcattaag atcattcggg aaccatgacc tatcgcacca agcagcgcac accagcgtaa
     4921 cgctcgaacg ggaacgttga acaccatcgc aaagccgacc gcaggaatgg cggcgaggat
     4981 catatcctgc gccaacgcta acagaaattc gatcacaccc atccgcgtag cccccaaatc
     5041 gtcagtgcca ttactacgcc gacacaggta gccagtgtca gcagactggc gattgcccag
     5101 cgtgccagtc cggtattgat gtggccttta aacatatcgg cgacggcatt tatcaacgga
     5161 aagcccggca ccagcagcag aacgctggcg gccatcgcaa tggtgggggt attgctgaaa
     5221 gtgggaagtt gcaaaagcaa tccggaaatg gtggtggcgg cgaaagcggt gaggcaaaag
     5281 ttgatctgtg gatgaagatg acgttgtgcc agcagctggc ggatatacat cgcggccgta
     5341 ctggccagga aggtgatgac ggcaccatcc cagccacctt tattgagttt acagaaacag
     5401 gcgcaggaaa ggccaaccat taaggctacc agccatcttg ggtaacgtaa tggctgaatt
     5461 tggctaaatc gtttctctac gcctttgtaa tccagcagat gatgctccgc cagaattaca
     5521 atgtgctgga cttcagtcac cacatgcata ttaatgccgc gatcgtgatt tttacgtgtc
     5581 gatgtcaggc attgcccatc tttaatggtt gtcagcacta tggcgttcga agagatagaa
     5641 ctttcgacgc tgtccattcc caacgcccga cccagtcgtg aggaaagctc atcaaccaac
     5701 gcgctttccg caccatgttg caaaagaaat aatccacact ggatacaaag ccgtgttacg
     5761 gctcgctgtt gctcagtttg cataactcgc cctttgtcat agacgtgctg cgtattgttc
     5821 agataatgag aatccatttg tatcatgaag cacggttgtt ttgcggtagg ttaagatcaa
     5881 aattgcgctg attaatcctg gttatcggga gatgctcggt gataaaaagc gaattatctc
     5941 tcgccgtaaa caaactatcg catgaatatt atcactataa tgaatttgtt atttggcctt
     6001 tacgagtcag gataatagat agctgggcgc gttttttaat gttaacaagt taaaaccatc
     6061 agattttat
//
LOCUS       DAZFFE010000181         5810 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFFE010000181 DAZFFE010000000
VERSION     DAZFFE010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.182"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..374
                     /locus_tag="ACHJ69_004603"
     CDS             <1..374
                     /locus_tag="ACHJ69_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO5017420.1"
                     /translation="PYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLAS
                     YLITHSGNYRYNSTESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLF
                     KKIAVKNRTQAVSWANDNLRR"
     gene            379..768
                     /gene="csgE"
                     /locus_tag="ACHJ69_004604"
     CDS             379..768
                     /gene="csgE"
                     /locus_tag="ACHJ69_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO5017421.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            793..1209
                     /gene="csgF"
                     /locus_tag="ACHJ69_004605"
     CDS             793..1209
                     /gene="csgF"
                     /locus_tag="ACHJ69_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO5017422.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1236..2069
                     /gene="csgG"
                     /locus_tag="ACHJ69_004606"
     CDS             1236..2069
                     /gene="csgG"
                     /locus_tag="ACHJ69_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO5017423.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2133..2624)
                     /gene="ycdZ"
                     /locus_tag="ACHJ69_004607"
     CDS             complement(2133..2624)
                     /gene="ycdZ"
                     /locus_tag="ACHJ69_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO5017424.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2726..3280)
                     /gene="ycdY"
                     /locus_tag="ACHJ69_004608"
     CDS             complement(2726..3280)
                     /gene="ycdY"
                     /locus_tag="ACHJ69_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO5017425.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3304..4041)
                     /gene="ycdX"
                     /locus_tag="ACHJ69_004609"
     CDS             complement(3304..4041)
                     /gene="ycdX"
                     /locus_tag="ACHJ69_004609"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO5017426.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTKAMIATIASGNVHIISHPGNPRYPIDFKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4096..5034)
                     /gene="ghrA"
                     /locus_tag="ACHJ69_004610"
     CDS             complement(4096..5034)
                     /gene="ghrA"
                     /locus_tag="ACHJ69_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO5017427.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGLQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY"
     gene            5268..5355
                     /locus_tag="ACHJ69_004611"
     tRNA            5268..5355
                     /locus_tag="ACHJ69_004611"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5302..5304,aa:Ser,seq:gga)
     gene            complement(5505..5810)
                     /locus_tag="ACHJ69_004612"
                     /pseudo
     CDS             complement(5505..5810)
                     /locus_tag="ACHJ69_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
BASE COUNT         1549 a         1457 c         1352 g         1452 t
ORIGIN      
        1 acccgtaccg cgacattgaa aactggcctc atatcaacgg cgttttttat gccatggagg
       61 atcaagaacg tgttgtcaat gggttgcaag gcgtcctgcg cggcgaatgc tactttacgc
      121 aaaagcttgc cagttacctg attacgcatt caggtaacta tcgttataac agcacggaat
      181 cagccctcct tactcatcgg gaaaaagaga tcctgaataa gctgcgtatc ggcgcgtcta
      241 ataacgagat cgctcgttcg ttgttcatca gcgaaaatac ggttaaaacg catctttata
      301 atcttttcaa gaagatagcc gtaaaaaacc ggacacaagc ggtttcctgg gcaaacgata
      361 acctcaggcg ataaagccat gaaacgttat ttacgctgga ttgtggcggc agaatttctg
      421 ttcgccgcag ggaatcttca cgccgttgag gtagaagtcc cgggattgtt aactgaccat
      481 actgtttcat ctattggcca tgatttttac cgagccttta gtgataaatg ggaaagtgac
      541 tatacgggta acttaacgat taatgaaagg cccagtgcac gatggggaag ctggatcaca
      601 ataacggtga atcaggacgt tattttccag acttttttat ttccgttgaa aagagacttc
      661 gagaaaactg tcgtctttgc actgattcaa actgaagaag cactaaatcg tcgccagata
      721 aaccaggcgt tattaagtac gggcgatttg gcgcatgatg aattttaaat aaaaaattgt
      781 tcggaggctg caatgcgtgt caaacatgca gtagttctac tcatgcttat ttcgccatta
      841 agttgggctg gaaccatgac tttccagttc cgtaatccaa actttggtgg taacccaaat
      901 aacggtgctt ttttattaaa tagcgcccag gcacaaaact cttataaaga tccgagctat
      961 aacgatgact ttggtattga aacgccatca gcgttagata actttactca ggccatccag
     1021 tcacaaattt taggtgggct actgtcgaat attaataccg gtaaaccggg ccgcatggtg
     1081 accaacgatt atattgttga tattgctaac cgcgatggtc aattgcagtt gaacgtgaca
     1141 gatcgtaaaa ccggacaaac ctcgaccatc caggtttcgg gtttacaaaa taactcaacc
     1201 gatttttaag ccccagcttc ataaggaaaa taatcatgca gcgcttattt cttttggttg
     1261 ccgtcatgtt actgagcgga tgcttaaccg ccccgcctaa agaagccgcc agaccgacat
     1321 taatgcctcg tgctcagagc tacaaagatt tgacccatct gccagcgccg acgggtaaga
     1381 tctttgtttc ggtatacaac attcaggacg aaaccgggca atttaaaccc tacccggcaa
     1441 gtaacttctc cactgctgtt ccgcaaagcg ccacggcaat gctggtcacg gcattgaaag
     1501 attctcgctg gtttataccg ctggagcgcc agggcttaca aaatctgctt aacgagcgca
     1561 agattattcg tgcggcacaa gaaaacggca cagttgccat taataaccga atcccgctgc
     1621 aatctttaac ggcggcaaat atcatggttg aaggttcgat tatcggttat gaaagcaacg
     1681 tcaaatcggg cggggttggg gcaagatatt ttgggatcgg tgccgacacg caataccagc
     1741 tcgatcagat tgccgtgaac ctgcgcgtcg tcaatgtgag taccggtgag atcctctcct
     1801 cggtgaacac cagtaagacg atactttctt atgaagttca ggccggggtt ttccgcttta
     1861 ttgactacca gcgcttgctg gaaggggaag tgggttacac ctcgaacgaa cccgttatgc
     1921 tgtgcctgat gtcggctatc gaaacagggg tcattttcct gattaatgat ggtattgacc
     1981 gtggtctgtg ggatttgcag aacaaagcag aacggcagaa cgacattctg gtgaaatacc
     2041 gccatatgtc ggttccaccg gaatcctgac agataaaaaa gccggaatta tcatccggca
     2101 ttttacggtt taagcgaaag cgtgagtgcc cctcacgctt tatttttgat ttcctgctca
     2161 cggtgcgccg tctttgcact acgcgccgcc aaccacaggc cgctgttttt cattgcgtaa
     2221 ccaaatacca gtccaagcgc cagcgaaggt aacaccagtt tccagtcacc ctgcccggca
     2281 aacgtcgcgc atgcgcctat aaacgtcccc ggaacaaatg aaagcagcag ctgtttggcc
     2341 tggatacaca tcagaaacgc gacaataccg gttatgacat aaccgagtat ttccagatgt
     2401 ggtgccaatg cactaccgta aataatgacc atcgcccaca ccacgccact aagcagggtt
     2461 gcagcggaga tcgccagccc tttcagccca ccttgcgggc aggcaaagta ggccgtacag
     2521 cccaggaagc ccgcccagct tagtaagccc agggaaacag ccacccatcc ccagataccg
     2581 gagagaatgc ctgttgtgat tgcaatagag agaagtatgt tcatgccgcg catgatagca
     2641 gaaaacgcgg ccatgaatga gatcgagcac acattttatg caacaacaaa ttacccgttg
     2701 cagttatgag tgatgcagat cacatttact cttcagaatc ttcttccagc tcgtcccaca
     2761 ttgcactaat agcatcgcgg gttagcggtg ccatggtgcg ccagaaaggc gtagttgcat
     2821 gggcctccac tttgccaagg aacgcaccac accagggtaa cagatactca ctgaacagtg
     2881 tttccagtgc ttcgctctca tcttccgttg actgatcttc cagccaggaa gccgcaagca
     2941 gcaatgtgcc gatgtgatcg gctggcgtat ccgctagtgg catccctcgt tcggaaagaa
     3001 aagcgcgcac ttccgcttcc gttgcgccct caacccatgc gctacgatat ggcggcacag
     3061 cacattcatc gccgataaac aacgcattgt aatcggcaga gacttgcgtc atatcacagc
     3121 ttttctgtaa acgcgtcagt aactcatcct gctccagtgg ccagttcgca gccagtttcc
     3181 cctcacgaat cagggtaaac aacggcacca gtaaaggatc ttgcggttgg cggtaataga
     3241 gcgaacccag cacacgacag aggatagaaa actcattcat taatatattt ccattactag
     3301 taattaaagg tctgcaaatt ccgcaatcgg tgccataccg cgagattcaa ggaagttaag
     3361 taagcggcgc ggagaaacat tcaaaatgcg ctctggcgga aaatctaccg cgtcgaggat
     3421 tttaagacac tcttcaaatt cccccatggt aaacgcagtg tgagaatccg agcctaatgc
     3481 cacccaacct ccagcgtcac gtaccgctgc ggctaccgca cgacagttgt cttcactgcc
     3541 cttacgtgag tgtaaaaatg aggaattatt gatttccagc gccacctgat gtttcgctgc
     3601 agcttcagca acagctttaa aatcaattgg atatcgtgga tttccgggat gacttattat
     3661 atgaacattg ccgctggcga tagtggcgat catcgctttt gtattggtcg ctttgtcatg
     3721 tggcgcaaaa accggctcat gaaaaccggc aataatcaga tccagcgaat caaacatttt
     3781 gccgctgcag tcaatttcac catcaacatt tttaatgtta gcttcgatgc cgcgcaggat
     3841 ccctacccca tcaaccactc gcggccagat acgcatgtta atgaagtgcc agtgatgcgg
     3901 cgcatcttcc atatccgggc catgatcggt gatcgcaaaa agtttaatgc ccttttgttt
     3961 ggcctgggca atgtaatcac tcaatgtgct atatgcatgc gtgctggcaa cggtatgcat
     4021 atgaaggtcg acgggataca taactctctc ctgtattcaa ttcccgccaa ggatagcagg
     4081 aaacctgatg ctttattagt agccgcgtgc gcggtcgact tgcccgcaga ccctctcccc
     4141 tttttcgagc tgggcaatgg tgcgcgaaat gtaatccaca gcttcagcgg gacgggtaat
     4201 cgcggcgaca tgtggtgtta tcgtcacgcg tggatgttgc cagagcggac tttccggcgg
     4261 taagggttca cgattaaaaa catccagcat tgcgccttta actttgccgc tatccagcgc
     4321 cgcgagcagg tcatcttcca cgacatgaac accacgcgcc aggttgagga gatacgcgcc
     4381 atccggtaat ttttcgagta attgttgatt aataatgccg acggtttcag gggtattcgg
     4441 taacaaatta atcaataccc ggcattggct cagaaatgca gaaagttctt cccgtccggc
     4501 aaaactttgc aagccaggcc acgatttacg ggttcgactc cagcaacgca gcgggaagcg
     4561 ccaggtttgc agactctgtg caactttact gcccagtacg cctgcgccca aaatgccgat
     4621 ggtaaaatct tcccgatgat attcaggaag cggttgccaa tgcgaactat tttgctggat
     4681 gcgataatcg tcaaaacgtc gaaaccaatg cagcacctga ctgacagcat attcctgcat
     4741 ttgctcgccc ataccggtat cttccaggcg aaaaagtgga acagaaggct tcagcatttc
     4801 agggtgtgcc tgtagcttgc tcaaaataga atcaacaccg gccccgagtg cgaacaccgc
     4861 tttaagatcg cgccctgcca gcatttcaac tggaggatgc cagactaaag cataatcagc
     4921 agagtcatta tctccgcttt tccatgctct gactcttgcc tgaggaatag ctttgcgcag
     4981 tgcctcaatc caccattggg tatcgaacgt tgggtgataa aagatgatat ccatactgac
     5041 tcccgaaaag cgttgcgcga aatttattcg cacttatcgt tatgatctac aaaagccacc
     5101 agcataacaa atccgtggtc ggtggcaaaa aaagcaaatt tcgcttatta aaactgcata
     5161 ttgattgaag tttgaataat cgagcgattt tttaaaaaag tttgttgacc tcaggtcatg
     5221 atttccctaa attagcgccc gttccagcaa gacaggaacg acaatttggt gaggtgtccg
     5281 agtggctgaa ggagcacgcc tggaaagtgt gtatacggca acgtatcggg ggttcgaatc
     5341 cccccctcac cgccagatta caagcgcatg ttattgttta acatgcgctt ttttatttat
     5401 ccttccagac agtatgccac cgggtatgcc atctgaaacc ggtttatatg ccccacgatc
     5461 atatcttatc ggacaatttc cgagctttga gcatactggc aaagctatac tgccagcatg
     5521 tcaggatagt gtttttgcga taatatcatt cagtctgtca accagttctg gcttcctgaa
     5581 cgtgatgcgc gcagagccct tcttaaagta tttaataatg aacatctcat cttcatagca
     5641 ttcttttccc tgaagagacc ggatgtgatc gtccagatga atagttatgt tttccctgtt
     5701 gtcggggatc ggtttgccgc tgaacagatg cagcattctt tccaggtcgg caagtctatc
     5761 tgcctgttgc ccggtgataa gatgcaatcc ccaccggtcc cacctcatca
//
LOCUS       DAZFFE010000182         5778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZFFE010000182 DAZFFE010000000
VERSION     DAZFFE010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.183"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            49..1794
                     /locus_tag="ACHJ69_004613"
     CDS             49..1794
                     /locus_tag="ACHJ69_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO5017428.1"
                     /translation="MTPGEKRVASRLESFLNDDCLVWYDIPVGRKNRHPDFVIIDPEN
                     GLIFLEVKDWTISTLCKANQEQVTLETDGIQKSEINPLVQVRRYACDTVNALPTDPCL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKTLAGNNENAVEKIFPAAQTICQDEMTQS
                     VLPQVFRQKIAGMFTTGFRTRVPPRMRDILRAHLFPEVTVKQNSQTKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIEAKGL
                     TSRVTVSHFHSWCTSMIKRHGIQVTTDGKNYPEKCFSALEEAVNRGKITDTGYDAVLV
                     DEGHDFDSRWLALIARLFDNSSRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNPKCILHFSYAFSREYFEKHQNREIPLLQPQAGGEEGTEPKILRCTSEHDE
                     ARQVADWLEKRHALCGHWSDMAVLCPAEFSVKQLKDIMTQRGIPFTTCFDSEGKKTYS
                     RRRDVVHLLTYQSSKGLEFPYVAVINASFVHSGALDESEVIPVLYVAFTRATRELLVT
                     CYRENSISRHLEDFA"
     gene            1860..2082
                     /locus_tag="ACHJ69_004614"
                     /pseudo
     CDS             1860..2082
                     /locus_tag="ACHJ69_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2086..2379
                     /locus_tag="ACHJ69_004615"
     CDS             2086..2379
                     /locus_tag="ACHJ69_004615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017429.1"
                     /translation="MSSSAQWNIFAGYHTDILSVTINHDLIQQYQPLQNIPNEDKNNK
                     KPIKVWVLMTKFEKWEEQKCRILYQSSGVTEPRTASRLFSNQRITADGESLSL"
     gene            complement(2705..3976)
                     /locus_tag="ACHJ69_004616"
     CDS             complement(2705..3976)
                     /locus_tag="ACHJ69_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HFO5017430.1"
                     /translation="MKFRILILISFLTFIFPLTSFSAVKQKTSEQISSRVDMSMDNAP
                     LPQVISLIWQRVFNRPFQLSPELAGDTRLVSFYLTQSLEPRSFFISYLKNMGIAVSSR
                     NGVDWIYIPAKKEFKEPVSVFTYRPKYRSVSYLSSMLTSVTQSGSFSNHVQTVDYSGA
                     SASAASSGSAINSVSAATDAEVLVYSGTAADIRRVRDILPRIDIPAEQVTVSGYVLEV
                     QTTERNASGLQIIADLFKNRLGVSLGARLDDGNAFTLNVGGLNAFYSLIKTDSRFNIV
                     SNPRLTVLSGSQSQFTVGQEVPVLDSVTYQGNSGTPVQSVTYRNSGAIFTVTPVVLDG
                     VITLDISQQLSDFVKTTTGVNTSPTLTKREISTRVDVHDGDVLVLGGLASSKTTKARS
                     GFSFLPGFTGSSEDSDKTDIIVVLQADRVSR"
     gene            complement(3979..5091)
                     /locus_tag="ACHJ69_004617"
     CDS             complement(3979..5091)
                     /locus_tag="ACHJ69_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HFO5017431.1"
                     /translation="MSIFAYVGVPGSGKSYEVVSNVIVPAICEGRRVVTNIYGINYDE
                     ILDYAEKKKLLKDGIKPGEIICVDNERIIAPDFYPVHENQERSLCKPGDLIIVDECHR
                     FYENDKSLSKDAKIFAAEHRHYADPETGRTCDFILVNQSVTNLPRFFRDRIEKTFRMR
                     KLRQLGLSRSYCVDVYDGAKITKSRFLSNYVCKYSKDVFRLYSSHHVQNAQESTVDSR
                     GVLFKKRTIIFVLIFLSGTVWAVFHYVVAFFNPDENTLVNDVDSRTESQPADNTNLSL
                     NHAVNKPGVGTANLQTTPSDKWCIVGRFSDGRKNWIFVRDAENRLRMASASGFTGYNI
                     MAEGELDGEKITAWSCNKPVSVRSVSSSTLNTGAGG"
     gene            complement(5109..5396)
                     /locus_tag="ACHJ69_004618"
     CDS             complement(5109..5396)
                     /locus_tag="ACHJ69_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 family protein"
                     /protein_id="HFO5017432.1"
                     /translation="MWAILLAAVNTAIAFIFRAVVIKFVLFTAVYLFISEVVPLIISH
                     LPSAGNISELFSRLPSGVLWLLNVFSVDIFLPVIVSAMFTRFFIRRIPFLN"
     gene            complement(5417..>5778)
                     /locus_tag="ACHJ69_004619"
     CDS             complement(5417..>5778)
                     /locus_tag="ACHJ69_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017433.1"
                     /translation="TGTGTTTGTGTTTGTTTADGADIKVDFGSAPSVSLPDINSPPSG
                     TQIITPLWDMWPEARDFSVSIPAGTCPVFTFHIWDQDYTMDQFCTLLDNDSVRNTFRV
                     VMTLVSSLMAFFIVLKA"
BASE COUNT         1678 a         1389 c         1286 g         1425 t
ORIGIN      
        1 gcgtttatgg cgaaactgat aaatatatcg gcgtccactg aggagagaat gacgccagga
       61 gagaagaggg tggcttcacg actggagagc ttcctaaatg atgactgcct tgtctggtac
      121 gatattccag tgggacggaa aaaccggcat cctgattttg tcattattga cccagaaaac
      181 gggctgatat ttctggaggt caaagactgg acgatttcca cactgtgtaa agcaaaccaa
      241 gaacaggtta cgctggaaac cgacggtatt cagaaatcag aaataaatcc gctggtacag
      301 gttcgtcgtt atgcttgtga tactgttaat gccttaccta cagacccatg cttacggcag
      361 aatgacggcc agtataaggg acggctgaac ctagcttggg cgtatggtgt ggtgtttact
      421 cgtattaccc gtcagcaact gaagacactg gcaggaaata atgagaatgc agtggaaaag
      481 attttccctg cagcacagac aatctgtcag gatgaaatga cgcagtctgt tttacctcag
      541 gtattccggc aaaaaattgc cgggatgttt accacaggat tcagaacccg ggtgcccccc
      601 cgaatgagag atattctgcg tgcccatctt tttcctgaag tgaccgtaaa acagaactca
      661 caaacaaaaa tcatggatat tcaacaggag attctggcca gaaatattgg tgacgggcat
      721 cgtgtcattc atggagtggc cggttccgga aaaaccatga ttcttttgtt ccgttgcctt
      781 tacttggctg aaaccactcc cggaaagatt ctggtgctgt gttacaacat taccctagcc
      841 agctatctac gagaatgcat tgaagctaaa gggctgacgt cacgggttac ggtatctcat
      901 tttcacagct ggtgtacatc catgataaaa cgccacggta ttcaggtgac gactgacgga
      961 aagaattacc cggagaaatg cttcagcgca ctggaagagg ctgtgaacag aggaaaaatc
     1021 accgacaccg gatatgatgc tgtactggtg gatgaggggc atgattttga cagtcgctgg
     1081 ctggctctaa ttgcccgtct atttgataat tcatcccgtt cattactcct gatgtatgat
     1141 gacgcccagt ctatttaccg gcgtgagcgg gcactgaact tttcactggc cagtgtggga
     1201 atccaagctc aaggacgtac ttcagtcctg ccagtcaatt atcgtaatcc gaaatgcatt
     1261 ctgcactttt cttatgcatt ctcccgcgag tattttgaaa aacatcaaaa ccgtgaaata
     1321 ccgctccttc agccacaggc gggcggtgaa gagggcacag agccgaaaat tttgcgttgt
     1381 acttctgaac atgatgaagc cagacaggtt gcagactggc tggagaagcg tcatgccctg
     1441 tgcggtcact ggagtgatat ggctgttctg tgtccggcag agttttcagt gaaacaactg
     1501 aaagacatta tgacacaacg cggaatacct tttaccacct gctttgatag tgaaggtaag
     1561 aagacatact cgcgacgcag ggatgttgtg catttactga cgtaccagag cagtaagggg
     1621 ctggagttcc cgtatgtagc ggtcatcaat gcgtctttcg ttcattctgg tgcgttggat
     1681 gaatccgaag taataccggt gctttatgta gcgttcaccc gggcaacccg cgaattactg
     1741 gtgacctgtt accgtgaaaa cagtatcagc cgccaccttg aggattttgc ataatacctg
     1801 actatttctt tattccgagc tttcaccata ctgtctttat caggtttatt taattcgcca
     1861 tgaacccgtt atttaacgat attcagatgt gtctttttca cctgaagtat tctccatatt
     1921 cctggcgctg gaatatcagt tccagatctc aggaaactgt atatatcagc aatgatacct
     1981 gtacaatccg gtgttcattt aagcaagaaa ggacaacacc tattcactga acgttatacc
     2041 agacaaatta ataatctgtt accggttctg aaacggcagt gagatgtgtc atcatccgcg
     2101 cagtggaata tctttgccgg ctatcatact gacattcttt ccgtaacgat taatcatgat
     2161 ttaatacagc aatatcaacc actacaaaat atccctaatg aagacaaaaa caataaaaaa
     2221 ccaataaaag tatgggtttt aatgactaaa tttgaaaaat gggaagagca aaagtgcagg
     2281 atcttgtacc agagttcggg tgttacagaa cccagaaccg cttcacggtt attttctaat
     2341 cagcgcataa cagcagacgg tgaatcatta tcattgtgag ccgtcgtccg tcgtcacggc
     2401 aggcgctccc gtcctcgtac ctgcgggcgg gtgcggctgg cgttcctccg tcctactccc
     2461 tcccggcgtg ttggggtcac agaaaaatca tcaacatgcc acatcatagc atggacgact
     2521 cacgccttgc gtcgttcatc tcgacgggtg ccatgaaaac ccggctttgc aggaggcgcg
     2581 ttgcgctgtc ccgcggaata tacacgctct ccctggctcc ggccgttcag taactgcgtc
     2641 acatgaaacc actgcgcgcg gccttccatg tcctggcgga cataaagata accggtcatc
     2701 cggttcagcg cgatacacgg tcagcctgca aaacaacgat aatatccgtc ttgtcgctgt
     2761 cctcagagct gcctgtaaat cccggcagaa acgaaaatcc actgcgcgcc tttgtcgtct
     2821 tactgctggc cagtccgcca agaaccagaa catcaccatc atgcacatca acccgtgtgc
     2881 tgatttcacg ttttgtcagt gtcggactgg tgttaacacc cgtggttgtc tttacaaaat
     2941 cactcagttg ctgactgata tcgagtgtga tcacaccgtc gagaacaacc ggcgtcactg
     3001 taaaaattgc cccgctgttt ctgtacgtaa ccgactgaac cggcgttccg ctgttgccct
     3061 gataagtgac gctgtccaga acaggcactt cctgacccac cgtaaactgc gactgactgc
     3121 cggaaagcac cgtcagcctg ggattactca caatattaaa ccgcgaatcc gttttgatca
     3181 gactgtaaaa cgcattcagc ccccccacat tcagagtgaa cgcattacca tcatcaagac
     3241 gagcacccag cgaaacgcca agacgatttt taaacagatc agcgataatc tgtaatccgc
     3301 tggcgttccg ttcagtggtc tgcacctcca gaacgtaacc gctgacagtc acctgctcag
     3361 ccgggatatc tatccgtggc agtatgtcac gaacacggcg aatatctgcc gccgttccac
     3421 tgtatacaag gacttccgca tccgtggcag ccgaaacact gtttattgct gaaccggaag
     3481 aagctgcgga tgcagaagcg ccagaataat ctacggtctg tacatgattt gagaaagaac
     3541 ctgattgcgt gacagaggtc agcatggaac tgagatagct gacactgcgg tatttcgggc
     3601 gatacgtaaa cacgctgaca ggctccttaa attccttttt agccggaata taaatccagt
     3661 caaccccgtt tctggatgaa accgctatcc ccatattttt aaggtaggaa ataaaaaatg
     3721 aacggggttc cagtgactgc gtaagataaa aactcacaag ccgcgtgtcg ccagccagtt
     3781 ccggcgacaa ctgaaacggg cggttaaaaa cacgctgcca gattaacgat atcacctgcg
     3841 gaagtggcgc gttatccatt gacatatcga cacgagaaga aatctgctcc gacgttttct
     3901 gtttaacagc agaaaaggaa gtcaaaggaa aaataaatgt caggaaagaa atcaatatca
     3961 gtatacgaaa tttcattatt aaccccctgc ccctgtattc agtgtcgacg aagaaaccga
     4021 acggacggaa acaggtttat tacatgacca tgctgtgatt ttttctccgt caagttcccc
     4081 ttctgccatg atgttatacc ccgtaaaacc gctggctgat gccatgcgaa gacggttttc
     4141 cgcatcacga acaaaaatcc agtttttccg gccatcagaa aaacgtccga caatacacca
     4201 tttatcagaa ggtgttgtct gtaaattagc cgtacctacc cccggcttat taactgcatg
     4261 atttaatgac agattagtgt tatccgcagg ctgtgattca gttctggaat caacgtcatt
     4321 aaccagcgta ttttcatcag gattaaaaaa cgccaccaca taatgaaata cagcccagac
     4381 cgtacccgac agaaaaatca gaacaaaaat tattgttctt tttttaaaaa gcacaccacg
     4441 tgaatcaact gtagattcct gcgcgttctg tacatggtgg gaactgtaaa gtctgaaaac
     4501 atccttactg tatttacaga cataatttga cagaaacctg gatttggtta tcttcgcacc
     4561 atcataaaca tcaacacaat aactgcgcga aagccccaat tgcctgagtt ttcgcattct
     4621 gaatgtcttt tcaatacgat cacgaaaaaa acgcgggaga ttggttacag actgattaac
     4681 cagaatgaag tcacaagtgc gtcctgtttc agggtctgca taatgccggt gttccgccgc
     4741 aaagattttt gcatcctttg aaagactttt atcattctca taaaaacggt ggcattcatc
     4801 gacaataatt aaatcgcccg gtttgcacag tgagcgttcc tgattctcat ggacagggta
     4861 aaaatcaggg gcaataattc gttcattatc cacgcagata atttcacccg gtttaatacc
     4921 atccttaagc agttttttct tttccgcata atcaagtatt tcatcataat taatcccgta
     4981 aatattggtg acaacacgac gaccttcaca gatggcagga acaatcacat tactgacaac
     5041 ctcataggat ttaccggaac caggcacacc cacataagca aaaatggaca taatctgttc
     5101 ctctcctgtc agttaagaaa aggaatgcga cgaataaaga aacgcgtgaa catcgcagaa
     5161 acaataacag gaagaaaaat atcaacagaa aaaacattca gtaaccagag cactccggaa
     5221 ggcagacgag agaacagttc cgaaatatta cccgctgaag gtaaatgaga aataatcagc
     5281 ggcacaactt cagaaataaa aagataaacc gccgtaaaaa gcacaaactt aatcacaacg
     5341 gcacgaaaaa taaacgcaat tgcggtattc accgcagcaa gtaatatcgc ccacataata
     5401 actccttatt gcccggtcag gctttaagaa caataaaaaa agccataagg gatgaaacca
     5461 gcgtcatcac cacacggaag gtattacgca ccgaatcgtt atcaagcagc gtgcagaact
     5521 gatccatggt gtaatcctgg tcccagatat gaaaagtgaa taccgggcag gttccggctg
     5581 gtatggatac cgaaaaatca cgtgcttcag gccacatatc ccagagcggc gtaattatct
     5641 gtgtacctga aggcggagaa tttatatccg gcagagaaac cgacggagca gaaccaaaat
     5701 caaccttaat atctgccccg tctgccgttg ttgttccggt ggttgtccct gttccggtgg
     5761 ttgttcccgt gccggtgg
//
LOCUS       DAZFFE010000183         5687 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZFFE010000183 DAZFFE010000000
VERSION     DAZFFE010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.184"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..526
                     /locus_tag="ACHJ69_004620"
     CDS             <1..526
                     /locus_tag="ACHJ69_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017434.1"
                     /translation="VTYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQT
                     SPDVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYTSEYHNGRQVLDEIQTPSAI
                     NSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLTLGFFLSIVISVMFCL
                     VKKMVDEYQQNSGQ"
     gene            complement(769..1464)
                     /locus_tag="ACHJ69_004621"
     CDS             complement(769..1464)
                     /locus_tag="ACHJ69_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO5017435.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLTQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1457..2884)
                     /locus_tag="ACHJ69_004622"
     CDS             complement(1457..2884)
                     /locus_tag="ACHJ69_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO5017436.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2895..3614)
                     /locus_tag="ACHJ69_004623"
     CDS             complement(2895..3614)
                     /locus_tag="ACHJ69_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO5017437.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4142..4996)
                     /gene="rclR"
                     /locus_tag="ACHJ69_004624"
                     /pseudo
     CDS             complement(4142..4996)
                     /gene="rclR"
                     /locus_tag="ACHJ69_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
     gene            5223..>5687
                     /locus_tag="ACHJ69_004625"
     CDS             5223..>5687
                     /locus_tag="ACHJ69_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5017438.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPGGNLEIHGEKIFINTGAQAVVPPIPGITTTPGVYDSTGLLNLK"
BASE COUNT         1551 a         1440 c         1200 g         1496 t
ORIGIN      
        1 agtgacttac agtctaaaaa taaaccagac aacaccaggc atacttgtta gctgtgatag
       61 caataataac tttgcctgtc agaccacaat gaccgaagat gttattcagc gaattactac
      121 tttttttcag accagcccag atgtcaagaa cagagaaata aagctggaat ggtcaggaga
      181 taagagagat ttaccaactg ctgaagcgga aatatctcgc gtgcaggcgt ctatcattaa
      241 atggtatacg tcagaatacc ataatggcag gcaagttctc gatgagatac aaacgccttc
      301 agcaattaac agtgagcttt atacaaaaat gatatacctg accaggaact ggtcactgta
      361 tccgaacggt gatggctgtg tcactataag ctcaccagaa ataaaaaata aataccctgc
      421 tgccatttgc ctgactctgg gattttttct aagtattgtg atttctgtaa tgttttgcct
      481 tgtcaaaaaa atggtagatg aataccaaca aaactctggg cagtaataaa cagcattctg
      541 acagagagtg cctgtataaa actatagtga cagtgaccgg gaagggagcc cgtcttcagg
      601 aatggattcc tgtcttttta actgtaaata tcctggcatt attaatgtca ggatatttac
      661 agaagagcag cataaccatg acggaacggg gttaagtcag gttgtcctta acgttattca
      721 caacctcgta taccgccttc aggtttatgt ccagactaca tatctctctc agcaatcctc
      781 aataatcaga tacaccgctt tcaccggacc atgaaccccg acgactttga taagctcaat
      841 gtccgccgtt gaactgggac cactgatgat gttaatacat gacggcattc gttcaccggc
      901 ctgcgctttc tgatgcaatt tttctgcgag ttgcgctaca cgcggcagga ttgtgctttt
      961 acgcaggata aaaagagaag attccgggag caggctcaat gaacgcccgc gcccggcggc
     1021 ggaaaaaaga accacgcctc ccgattcggt taaaccatat tcagcatata cgacgcccac
     1081 tttagcctgc tctgcttgtg atatgttctc aatacctttc gccggatccc aaacaacggc
     1141 attgcattcc tgctgcaaac gttcgctaat ccccaattcc tccagcctcg tgtcaccact
     1201 aatcacgacc gactgatctc ccagctcttt acacagacgt attgcagctt ctgccgcctt
     1261 cgcctcgctg gtcagttcac agcgcgtcaa cataacatcg ctggcaaact ggataaacgc
     1321 gtcacagcgc tgttgttggg taagttgggt aagccgctcg ttagcatagt tgttaagcgg
     1381 cgcatcttct gcttgcggtt caagtcgcag cgggcgaccc agtgcctgag caacgttatt
     1441 caaaaattcg ctccgattat ccattctttt tctcctgcgc ctgatgtttc ttaaaccaac
     1501 tacggaaact ctctccgtca gcttcaggaa gatcgcgtgc ttccatccag tcgctaatcg
     1561 cgccaaattt gagtggtgtt ttgccgccat tgataaacca gcttgccgca tgagcaccag
     1621 ccatcatccc aactttccac aatcctggat gactattggc ataagcgaac atttttatcg
     1681 cccgttgctc tgcttttgcg gtgatccctt tttcagccat cacccgacga tgacgcaaaa
     1741 tcagttttga cagcggaata cgcaccggac acacgctgtc acaagcggtg cataaagagc
     1801 aggcgtaggg taaatcttta aaatctttat agccgccaag taacggggaa atcaccgcac
     1861 caattggccc tggataaata gagccatatc catgaccgcc aatatggcga tatgccggac
     1921 aagtattcat acaagccccg caacgaatac agcgcagcac atcccgaaat tcagaggcca
     1981 gcacctcaga acgcccgtta tcgacaataa ccagatgaaa ctcttcagga ccatcaacgt
     2041 gccccgcttc gcgcggtcct gtcagccagg tgttgtatcc cgtcaaacgt gcaccaacgg
     2101 cactgcgcgc cagcatggtg atcaatacat ctacctctgc aaacgtggga gcaatacgct
     2161 ccattcccat cactgcaata tgcgttttag gcagcgtggt acacattcgc gcattacctt
     2221 cattggtcac caggcatacc gaaccggtct ctgccaccgc gaaattacag ccggtaatac
     2281 ctatttcagc actgaggaaa tcttcgcgga ttttttgccg aatgaataag gtcatcgctt
     2341 caggcgtttc cggcccctca tagcccagac gttcgtgtag cactcgacgg atctgatggc
     2401 gatctttatg aattgccggg accacaacat gagagggcgg atcttgatcc agctggagaa
     2461 tgtattcacc cagatcggtt tcaatcacct gaatcccggc atcctgcaac acatgattga
     2521 caccaatctc ttcggtcacc atcgatttag atttcactac cttccgggca tttttgcgtt
     2581 gggcaacctg taaaatgtag cgggtggcgt cttctttggt ttttgcaaaa tagacgtgac
     2641 cgccgttttg tgtcactttt tctgagagct ggtacagata agcgtcgaga ttactcagaa
     2701 catgatcacg tatctgggcg gcccgatcgc gccactcctc ccagtgcccc aattcatcga
     2761 ccattttttg ccgatttgcc ccaatacgct gctgcgcgtt tgccaccgct ttacgcatga
     2821 tcggatcttc aatttgctga cggatgcgtg tcttaaaatc tgtattactg gttttgatcg
     2881 acatctttat atcctcagcg gctcatcagc acttcagcaa tatgcatcac tttgactttc
     2941 tgcccttccc gttgtaatcg tccactgatg ttcagcaggc aactcacgtc agcaccaatt
     3001 aaatactcag ggcggacttc catcaggtgc gcaacctttt ctttcaccat ctcgccggat
     3061 atttcggcca ttttgaccga gaacgtgccg ccaaatccgc agcaggtatc ctgttcagca
     3121 aaggtcaaca gctccagtcc acgcacattt ttcagcagcg taagtggttc gtccttcacc
     3181 cccagcttac gggccaggct acaagatggg tgatacaccg ctctcccttg caaactggca
     3241 cctacatcga ctacccctaa tgtattaaca ataaaagagg tgagatcctg catacgcgcg
     3301 gcaacctttt cggcacgtga tgcccattca ggttcatccg ccagatacgt cgggtaactt
     3361 tttacggcat aggtgcaaga gccagccggt gaaataatgg gatcgtcgtt atcctccagt
     3421 gcggcgatca gatttttcat ccctggaatc gcttctttga tataaccact attgatcgca
     3481 ggctgaccgc agcatccctg tttctccggg aaatttacgc gacagccgag tttttccagt
     3541 agcagcacgg agtctcgtgc cattcttgat ttcagggcgt caccaataca ggtgacaaag
     3601 aaattgacat tcacaactaa tactccatta cttcatgccc atttatgccc gatggttccc
     3661 cccttccggg ggatcaatca tcaataaata tggcgttctt ttttaattgg ctagcaacat
     3721 accacccctc atttgtatga caaatgataa tctttcatct aaacttcaaa tgcaataact
     3781 aattttattt ctttcggcat cgctcacatt ttatagggaa atcgacgatc atttttggca
     3841 taagaaatcc atttaaaaat ggctacatcc cttataccta ttcagagcta cttattcagg
     3901 gattattgag atatacactg atggtgtaca aaaaaaatca ccgacaataa tcacgtatca
     3961 agtaatagtc gggattttat ttaattgaag aaatttagtt tgcattttta tttaaaaact
     4021 gagctagagt ccatcaaaaa aatatataaa acccaaaacc accgttggaa atagaaatag
     4081 ttgaaaggct cacaactccc ggaatgcgga ttatctttgc cctgatttct ggcggtttta
     4141 ttcagggtgc aagctgtctc accctttccc gatattctcc cggagtacaa ccaaactcgc
     4201 ggacaaacgc cttgtgaaaa gatgattcgc tggcatagcc tactgactca gcgatcacca
     4261 caacagggag catttcccgg gaaaacatct gggccgctat ttgtagacgc aactttgtta
     4321 atacagccag cggcgtggtt ccggaaacat cacggaaaag ctgggcaaaa cttgcccggg
     4381 acatgtgggc gatgctggcc agcgattcga cggtccaggc gtgtccaggc atttccatca
     4441 tttgctgtat taccgcacca agacgtggat gcagaagcaa actgagaatg tttttctctg
     4501 tattaacctg tgcaatccat tcacgcaccg cgagggtaaa gaatgtagcg cagatctggc
     4561 tacacagagc atccactccc ggcattgccg atctggattc ctgttgtaaa aacgggatcg
     4621 cctccctcag ccagttatat tccacgctgt ggttaaccgg tgctacaaac agcgtttccg
     4681 gcaaagacgt taaaaaataa cgcgccgaat gttgcagccg aagagttcca cagacaatac
     4741 aggtcgattc attatcgaca tgactcaggc gatgagctga attttatggt agcaggacca
     4801 catttcccgg gcgtaatgta aaaatctctc ccgtcggcat ttccagcttc gccgctcctt
     4861 gcgttaacgc atgccaacga ataaccgaca attccccggc accatgtgga agttgccagt
     4921 cacttcctaa cacgcaattc ttatcgatcg ttccttgtgg agcgttaagc atcaacaaac
     4981 ggctaagagc atccatatca gactcctgag tggttttttg attacaaaca ccaacaatct
     5041 tagtcaataa ttaatctaat accatcaaat agttaatcat gatttttgtt ttgtgattct
     5101 gattaagaaa aagaaaatac cagacgataa gacaaaatat caagcgttgt gaagaaatgt
     5161 tatttgctct tttgcgtcta tagtcatgat gtcaaatgaa cgcgtttcga caggaaatca
     5221 tcatgaataa atatcaggca gttattattg gttttggcaa ggctggaaaa acattagccg
     5281 tcacgctggc aaaagcaggt tggcgtgtgg ctctcatcga acaatcaaat gcaatgtatg
     5341 gcgggacctg tattaatatc ggctgtatcc caaccaaaac attggttcat gacgcacagc
     5401 agcacacaga ttttgtccgt gccatacagc gtaaaaatga agtggttaat tttttacgta
     5461 ataagaattt tcataatctt gcggatatgc ccaatatcga cgtgatcgac ggccaggcgg
     5521 agtttatcaa taatcatagc ctgcgtgttc atcggcctgg gggaaatctg gagattcatg
     5581 gcgagaaaat ttttattaat accggtgcac aagccgtggt tccgccaatt cctggaatta
     5641 ccaccacgcc tggtgtatat gacagcaccg gattacttaa tctaaaa
//
LOCUS       DAZFFE010000184         5543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZFFE010000184 DAZFFE010000000
VERSION     DAZFFE010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.185"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            183..1115
                     /gene="rfaD"
                     /locus_tag="ACHJ69_004626"
     CDS             183..1115
                     /gene="rfaD"
                     /locus_tag="ACHJ69_004626"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO5017439.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1125..2171
                     /gene="rfaF"
                     /locus_tag="ACHJ69_004627"
     CDS             1125..2171
                     /gene="rfaF"
                     /locus_tag="ACHJ69_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO5017440.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGVMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGYEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2175..3167
                     /gene="rfaC"
                     /locus_tag="ACHJ69_004628"
     CDS             2175..3167
                     /gene="rfaC"
                     /locus_tag="ACHJ69_004628"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO5017441.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAKNYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIIINKLTSEIR"
     gene            3164..4378
                     /gene="rfaL"
                     /locus_tag="ACHJ69_004629"
     CDS             3164..4378
                     /gene="rfaL"
                     /locus_tag="ACHJ69_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFO5017442.1"
                     /translation="MTSTLFFSFEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLVQSPDAGAGMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDIEKIVFFSFISALGLRCFSELIAYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFAVLSAIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGYGNVAYKDVYNKRVIDYPQWTFRQSIGPHNFALFIWFGTGLL
                     GLVSLMMLYCAILKECIKNVVQNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLG
                     VYAGLLLAMKNK"
     gene            complement(4413..>5543)
                     /locus_tag="ACHJ69_004630"
     CDS             complement(4413..>5543)
                     /locus_tag="ACHJ69_004630"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFO5017443.1"
                     /translation="IIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIKNPGY
                     SNYTFVNDNCSIHRVGFSRTYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVIVVH
                     NSMKLYSQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADIE
                     IVPNGIDLETYQSTFQPITRSELNISAEEKIIFYAGRIVPDKGILLLMQSFEKLAAAH
                     KNLKLVVIGDYTEMSKSDKGAYQRNVREIANRLKDRCIMLGCIPPEKMHCYYPLADLV
                     VIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDI
                     LKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT         1530 a         1222 c         1256 g         1535 t
ORIGIN      
        1 gcactattca cgtgcaaatc caacatccgc catgaaggac tggctaaaac ccaaactagt
       61 ttgttgcaat tagcatcctt gcatctctat gtaaagggct gaagggattc ggatgtgatg
      121 gtatgattac agacattcgt gtctgagatt gtctctgact ccataattca aaggttacag
      181 ttatgatcat cgttaccggc ggcgcgggct ttatcggcag caacatcgtt aaagccctga
      241 atgacaaagg catcaccgat attctggtgg tggataacct gaaagacggc accaagtttg
      301 tgaacctggt ggatctggat atcgcggact atatggataa ggaagacttc ctgatccaga
      361 ttatggctgg cgaagagttc ggcgatgtcg aggcgatttt ccacgaaggc gcgtgctctt
      421 ccaccaccga gtgggacggc aagtatatga tggataacaa ctatcaatac tccaaagagc
      481 tgctgcacta ctgtctggag cgcgaaatcc cgttcctgta tgcctcttcc gcagccactt
      541 acggtggacg cacctccgac tttattgaat cccgcgaata tgaaaaaccg ttgaatgtct
      601 acggttactc aaaattcctg tttgatgaat atgttcgcca aatcctgcct gaagcgaact
      661 cacagattgt tggcttccgt tatttcaacg tttatggacc gcgtgaaggc cataaaggca
      721 gcatggcgag cgtcgctttc catctcaaca cccagcttaa taacggtgaa tcaccgaagc
      781 tgttcgaagg tagcgagaac tttaaacgtg acttcgtcta tgtgggcgat gtggcagatg
      841 taaacctgtg gttcctggaa aatggcgttt ccggtatctt caacctcggc accggtcgtg
      901 cggaatcctt ccaggcagta gcagatgcta cacttgctta tcacaagaaa ggccaaatcg
      961 aatacattcc gttcccggat aaactgaaag gccgctacca ggcgttcact caggcagatc
     1021 tgacgaatct gcgcgcggcg ggttacgata aaccgttcaa aaccgttgcc gaaggcgtaa
     1081 cggaatacat ggcctggctg aatcgtgacg cataagagct ctgcatgaaa atactggtga
     1141 tcggcccgtc ttgggttggc gacatgatga tgtcgcaaag tctctatcgc acgctccagg
     1201 cgcgctatcc ccaggcgata atcgacgtga tggcaccggc atggtgccgt ccattattat
     1261 cgcggatgcc ggaagttaac gaagcgatcc ctatgcctct cggtcacgga gcgctggaaa
     1321 tcggcgaacg ccgcaaactg ggtcatagtc tgcgtgaaaa gcgctacgac cgcgcctacg
     1381 tcttacctaa ctccttcaaa tccgcattag tgcctttctt cgcaggtatt cctcatcgca
     1441 ccggctggcg cggcgagatg cgctacggtt tactcaacga tgtacgcgtg ctcgataaag
     1501 aagcctggcc gctaatggtg gaacgctata tcgcgctggc ctatgacaaa ggcgttatgc
     1561 gtaccgcaca agatctgccg caaccattgt tatggccgca gttgcaggtg agcgaaggtg
     1621 aaaaatcata tacctgtaat caattttcgc tctcatcaga acgtccgatg attggttttt
     1681 gcccgggtgc ggagtttggt ccggcaaaac gctggccaca ctaccactat gcggagctgg
     1741 caaagcagct gattgatgaa ggttatcagg tagttctgtt tggctcggcg aaagatcatg
     1801 aggcgggcta tgagattctt gccgctttga ataccgagca gcaggcatgg tgtcggaatc
     1861 tggcgggaga aacacagctt gatcaagcgg ttatcctgat tgcagcctgt aaggccattg
     1921 tcactaacga ttctggtctg atgcacgttg cggcggcgct caatcgtccg ctggttgccc
     1981 tgtatggacc aagcagcccg gacttcacac caccgctatc ccataaagcg cgcgtgatcc
     2041 gcctgattac cggctatcac aaagtgcgta aaggtgacgc tgcggagggt tatcaccaga
     2101 gcttgatcga cattactccc cagcgcgtac tggaagaact caacgcgcta ttgttacaag
     2161 aggaagcctg acggatgcgg gttctgatcg ttaaaacatc gtcgatgggc gatgttctcc
     2221 acacgctgcc cgcactcact gatgcccagc aggcaatccc agggattaag tttgactggg
     2281 tggtggaaga agggttcgca cagattcctt cctggcacgc tgccgttgag cgagttattc
     2341 ctgtggcaat acgtcgctgg cgtaaagcct ggttctcggc tcccataaaa gctgaacgca
     2401 aagcgtttcg tgaagcgcta caagcaaaga actatgacgc agttatcgac gcccaggggc
     2461 tggtaaaaag cgcggcgctg gtgacgcgtc tggcgcatgg cgtaaagcat ggaatggact
     2521 ggcaaaccgc tcgcgaaccg ttagccagcc tgttttacaa tcgtaaacat catattgcaa
     2581 aacagcagca cgccgtagaa cgcacccgcg aactgtttgc caaaagtctg ggctatagca
     2641 aaccacaaac ccagggcgat tatgctatcg cacagcattt cctgacgaac ctgcctacag
     2701 atgctggcga atatgccgta tttcttcatg caacaacccg agatgataaa cactggccgg
     2761 aagaacactg gcgagaattg attggtttac tggctgattc aggaatacgg attaaacttc
     2821 cgtggggcgc gccgcatgag gaagaacggg cgaaacgact ggcggaagga tttgcttatg
     2881 ttgaagtatt gccgaagatg agtctggaag gcgttgcccg cgtactggcc ggggctaaat
     2941 ttgtagtgtc ggtggatacg gggttaagcc atttaacggc ggcactggat agacccaata
     3001 tcacggttta tggacctacc gatccgggat taattggtgg gtatgggaag aatcaagtag
     3061 agtgtcgttc tacaagtatg tctcttgcag atttgccagc tcaaaccgtt tttcaaaatt
     3121 taaatcttga aataataatc aataagttaa catcggagat aagatgacct caacattatt
     3181 tttctctttc gagaaaaaaa actggatagc gtactggaac agagctcttg tattcttatt
     3241 cattaccacc tattttttgg gtgggattac aaggtataaa catcttattg ttattcttat
     3301 gacaataacg acaatcgtct atctctgcaa acggccaaaa cattatctat cattatttaa
     3361 aacatttctt tttggtagtg ttgccatatt aactattgct gcattgctat cacttgttca
     3421 atccccagat gcaggtgctg gcatgaagga aattttcaaa gctatcattg aaaatacttt
     3481 gctatgcaca atagcaatac cggttatatt gagagacgag aaaagagaag atatcgaaaa
     3541 aatcgttttc ttctcattta ttagtgcgtt aggcttacgc tgtttttctg aattgattgc
     3601 ctattataag gactatcaac aagggataat gccattcgca gattatagac accgtagcat
     3661 ttctgactcg atggtttttt tattccctgc attgttaaat ctctggctta tcaaatcagc
     3721 aaaataccgc atttcttttg cggttctaag cgctattttt atttttctga tattaggaac
     3781 tttatccaga ggggcctggc tttccgtgtt agtcattgga ttaatatgga ttctgatgtt
     3841 taaacaatgg aagttactat tagtaggagt aatggtttcc atcattgcat tgtcggttat
     3901 tttcacacat aaggagatga ccgcaaagct aacgtataaa cttcaacaaa ctaatagttc
     3961 ttatcgttat gcaaatggta ctcaaggcag cgcactcgat ctaatattag aaaatcctgt
     4021 tattggttac ggttacggtt acggtaacgt tgcatataaa gatgtctata ataaacgagt
     4081 cattgattat ccgcaatgga cctttagaca atcgataggg ccacataatt ttgcgctatt
     4141 catctggttt ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat
     4201 actaaaagag tgtataaaaa atgtcgtcca gaataaatac cgctcaccat ataatgcata
     4261 ttatataatc ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat
     4321 tgaaccaaat ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa
     4381 aataaaaagg ctgcataatg cggccttttt atttattcaa accaattatg aataacctct
     4441 tcaaatcttt gagttacgcc atcccaactg tagtgatcaa acacaaaatc ctgaccttgc
     4501 tttgcaactg ctgttaattc aggattcgct aacgttttta aaatatcgct gcttatagag
     4561 tctgcagtca taggttcttt taaatggaat cccgtagtat tttctttgac aaattccgtc
     4621 atccccccac gcgtactcac taaaacaggc ttaccagccc ccatagcttc aattgccacc
     4681 atgcaaaatg gttcctggaa ctgggaagga atgacgacta aatctgccag agggtaataa
     4741 caatgcatct tttcaggcgg tatgcatcct aacataatgc aacgatcctt caaacggttt
     4801 gcaatttccc tgacattgcg ttgatatgca cctttgtcac ttttactcat ttctgtataa
     4861 tcaccaatca caactaattt taaatttttg tgagcagctg ccaatttttc gaatgattgc
     4921 atcaacagta gtatgccttt gtcagggacg atacgcccag cataaaaaat aattttctct
     4981 tcagcggaga tattaagttc gcttctagtg atcggctgaa aagtagattg ataggtttct
     5041 aaatcaattc catttggtac aatctcaata tctgcatttg ctaaataaga ttggtaatac
     5101 tttttcaagt ataaactagg cactatcatt ttaacatttt gctcaagcaa ttttggttca
     5161 aatgcattat gcatatgtat aaccactctt gcctgaggtg ctcgcttacg aatttgagaa
     5221 tatagcttca tgctattatg aactacaata acactttctt tcgttatagg aaaatcatgt
     5281 gcaatattca atattctctg tgaatatgga agaggatcaa gtcttgtcca tttttgaaat
     5341 aaccgcttat atgtgcggct gaatccaacc ctatgaatac tacaattatc attaacaaag
     5401 gtataatttg agtatcctgg attcttgata caaacaatac gatttgggaa attggtacgc
     5461 tgagcaacct ggtacatcca cgtttcaaca gcggccgctc cgcgaggagg aattgaaaaa
     5521 ataggagtaa ctgtaaatat tat
//
LOCUS       DAZFFE010000185         5495 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZFFE010000185 DAZFFE010000000
VERSION     DAZFFE010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.186"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            35..1054
                     /locus_tag="ACHJ69_004631"
     CDS             35..1054
                     /locus_tag="ACHJ69_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5017444.1"
                     /translation="MNNEQMLIKCKDPRKCRFYAEYLNHKYPNEFPQFAPDNPQNCIR
                     NVNSFLKEKYYKASNNSFGVFGSTAEEALSKEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
     gene            1164..2036
                     /locus_tag="ACHJ69_004632"
     CDS             1164..2036
                     /locus_tag="ACHJ69_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO5017445.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            2369..5491
                     /gene="agn43"
                     /locus_tag="ACHJ69_004633"
                     /gene_synonym="flu"
     CDS             2369..5491
                     /gene="agn43"
                     /locus_tag="ACHJ69_004633"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFO5017446.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARTRGKRGGVAVALSLAA
                     VTSLPVLSADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSTGQMVGGTAESTTIN
                     NNGRQVIWSSGVSRDTLIYTGGDQTVHGEAHNTRLEGGNQYVHKYGLALNTVINEGGW
                     QVVKAGGTAGNTTINQNGELKVHAGGEASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLADS
                     GAAVSGTRSDGTAFRIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGSLA
                     GTTTLNNGATLTLSGKTVNNDTLTIREGDALLQGGALTGNGRVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNNSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLTSGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSARTEKFVPTTLQVKNLNGQNGTISLRVRPEMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVVEAINGATTEEGAFVQGNML
                     QAGAFNYTLNRDSDESWYLRSEERYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNG
                     ENNSVRLSIQGGHLGHDNNGDIARGATPESSGSYGFVRLEGDLLRTEVAGMSLTTGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKASS
                     GNNDFRARGWGWLGSLETGLPFSITDNLMLEPRLQYTWQGLSLDDGKDNAGYVKFGHG
                     SAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSINGSSAEGY
                     SSQATLNVTF"
BASE COUNT         1406 a         1405 c         1506 g         1178 t
ORIGIN      
        1 ttttgttata agagaggcga catggagtga gataatgaat aacgaacaga tgcttatcaa
       61 atgcaaagat ccccgtaaat gtcggtttta tgccgaatat ctgaatcaca aataccccaa
      121 tgaattccct cagtttgcgc ctgacaaccc acaaaactgt atcagaaatg ttaattcgtt
      181 tttaaaagaa aagtattata aagcttcaaa caattctttt ggtgtttttg gttcgactgc
      241 agaagaagct ctcagcaaag aaattaatga acttgaaatt attgcagggc aggcaatact
      301 gcctgaagag agttttctct ggttgaaaac tgacgaactt gcgactttct ttacctggac
      361 tactatttat ctgtcgaagg aagaccatac aaaattcccg aaaataaacc ataattcatc
      421 aggaataaaa aacggaattg aagtcaggaa aatacgaaca gttaccgatg aaacgttaaa
      481 tacttacaaa aaaagtaacc ttccggataa tccatcatcc cataacgaac gaataaaagt
      541 aatcatttca tattttgact cctgccattc tttaattaat aaaaatgcaa aaagcatata
      601 cctcgaggaa ctcagacagg cgtggcttaa aaagaaaagc gcttgtcaaa agtttaagtg
      661 gctccttcct gacgatgatg aaatgtgtca gtggttctgg acaagactga aacaaaaaca
      721 ggatgaaaaa catccagtac ataacccagt gacccggtgg ttcataccat cttcaacgaa
      781 agaatattat cttgcctctc ttgtcgcctt tgctttatgg aaagaagcgc ctgataccat
      841 tgaactgttc agaacacgaa taaatcatgc ctggcatcag aaaaaacaaa aggacaaata
      901 tcggagtgat ggtaaaaagg ccattaatat acatataaga agcaatgtga aagaaatgct
      961 tgatgagctc agaactgttt acggtctgag aacaggagag ttttttgaaa tgctgattac
     1021 agagaaatat cttgaacaca agcgaaggaa gtaattcatt acctgttaca ttctaaataa
     1081 tctcagacag atattatctc cgtgaatccc ccgccacctt tccggtgcgc ggggttttgt
     1141 cttttttcac cgggaataca tgtatgaatc cgtctgatgc cattgaggca attgaaaaac
     1201 cgctctcctc cctgccttac tcactttccc gtcacatcct ggaacatctg cgcaaactca
     1261 ccagtcacga acccgtgatt ggcattatgg gtaaaagcgg ggccggtaaa tcttcactct
     1321 gtaatgcact gtttcagggg gaggtcacac cggtcagtga tgttcacgcc ggcacccggg
     1381 aagtgcagcg cttccgtctg agtggtcacg gtcacagcat ggttatcact gacctgcccg
     1441 gggtgggcga gagccgggac agggatgcag agtatgaagc cctgtaccgt gacattctgc
     1501 ctgaactgga cctggtgctg tggctgatta aagccgatga ccgggccctg tctgtggatg
     1561 agtatttctg gcgacacatc ctgcagtgcg gacatcagca ggtgctgttt gtggtgacac
     1621 aggccgacaa aacggagccc tgccatgaat gggatatggc cggcattcag ccctctcccg
     1681 cacaggcaca gaacattcgc gaaaaaacgg aggcggtatt ccgtctgttc cggcctgtac
     1741 atccggttgt ggccgtatcg gcccgcaccg gctgggaact ggatacgctg gtcagtgcgc
     1801 tcatgacagc gcttcccgac catgccgcca gtcccctgat gacccgcctg caggacgagc
     1861 tgcgcacgga atctgtccgc gctcaggccc gtgagcagtt taccggtgcg gtggaccgga
     1921 tatttgacac agcggagagc gtctgtgttg cctctgttgc acgcacggtt ctgcgtgccg
     1981 tccgtgacac agtggtctct gttgcccgcg cggtatggaa ctggatcttc ttctgaacct
     2041 gttgtggatg atgtcctccc tgcctctgag tctgctcaca aaagcgctgt tttcgttacg
     2101 gtctctcttg tccgtgcaat agctcaataa tagaataaaa cgatcgatat ctattttatc
     2161 gatcgtttat atcgatcgat aagctaataa taacctttgt cagtaacatg cacagatacg
     2221 tacagaaaga cattcaggga acaacagagc cacacgtcag aaacttccgt cagccggacc
     2281 tccggcactg taacccttta cctgccggta tccacatctg tggataccgg cttttttatt
     2341 caccctcact ctgattaagg aaatgctgat gaaacgacat ctgaatacct gctacaggct
     2401 ggtatggaat cacattacgg gcgctttcgt ggttgcctcc gaactggccc gcacacgggg
     2461 taaacgtggc ggtgtggcgg ttgcactgtc tcttgccgcg gtcacgtcac tcccggtgct
     2521 gtctgctgac atcgttgtgc acccgggtga aacagtgaat ggcggaacac tggtaaacca
     2581 tgacaaccag tttgtatccg gaacagctga tggcgtgact gtcagtaccg ggcttgagct
     2641 ggggccggac agtgacgaaa acaccggcgg gcaatggata aaagcgggtg gcacaggcag
     2701 aaacaccact gtcaccgcaa atggtcgtca gattgtgcag gcaggaggaa ctgccagtga
     2761 tacggttatt cgtgatggcg gagggcagag ccttaacgga ctggcggtga acaccacgct
     2821 ggataacaga ggtgagcagt gggtacacgg gggagggaaa gctgccggta caattattaa
     2881 ccaggatggt taccagacca taaaacatgg cggactggca accggaacca tcgtcaacac
     2941 cggtgcagaa ggtggtccgg agtctgaaaa tgtgtccact ggtcagatgg tcggggggac
     3001 tgctgaatcc accaccatca acaacaatgg ccgacaggtt atctggtctt cgggggtatc
     3061 ccgggacact ctcatttaca caggcggtga ccagacggta cacggagagg cacataatac
     3121 ccggctggag ggaggaaacc agtatgtgca caaatacgga cttgcgctga acacggtaat
     3181 taacgagggg ggctggcagg ttgttaaggc aggtggtact gccggtaaca ccaccataaa
     3241 tcagaacggc gagctgaagg ttcatgccgg cggagaagcg tctgatgtca cccagaacac
     3301 gggcggtgca ctggttacca gtacagctgc aaccgtcacc ggcacaaacc gtctgggagc
     3361 attctccgtt gtggcgggta aggctgataa tgtcgtactg gaaaatggcg gacgtctgga
     3421 tgtgctgagc ggacacacag ccactaatac ccgtgtggat gatggcggaa cgctggatgt
     3481 ccgcaacggt ggtgccgcca ccaccgtatc catggggaat ggcggtgtac tgctggccga
     3541 ttccggggcc gctgtcagtg gtacccggag cgacggaacg gcattccgta tcggaggcgg
     3601 tcaggcggat gccctgatgc tggaaaaagg cagttcattc acgctgaacg ccggtgatac
     3661 ggccacggat accacggtaa atggcggact gttcactgcc agagggggct cgctggcggg
     3721 caccaccacg ctgaataacg gtgccacgct taccctttcc gggaaaacgg tgaataacga
     3781 taccctgacc atccgtgaag gtgatgcact cctgcaggga ggcgctctta ccggtaacgg
     3841 cagggtggaa aaatcaggaa gtggcacact cactgtcagc aacaccacac tcacccagaa
     3901 agccgtcaac ctgaatgaag gcacgctgac gctgaacaac agtaccgtca ccacggatgt
     3961 catcgctcag cgcggcacgg ccctgaagct gaccggcagc acagtgctga acggtgccat
     4021 tgaccccacg aatgtcaccc tgacatccgg tgccacctgg aatatccccg ataacgccac
     4081 ggttcagtcg gtggtggatg acctcagcca tgccggacag attcatttca cctccgcccg
     4141 cacagagaag ttcgttccga caactctgca ggtgaaaaac ctgaacggac agaatggcac
     4201 catcagcctg cgtgtacgcc cggagatggc gcagaacaat gctgacagac tggtcattga
     4261 cggtggcagg gcaaccggaa aaaccatcct gaatctggtg aacgccggca acagtggtac
     4321 ggggctggcg accaccggta agggtattca ggtggttgag gccattaacg gtgccaccac
     4381 ggaggaaggg gcttttgtcc aggggaatat gctgcaggcc ggggccttta actacaccct
     4441 caaccgggac agtgatgaga gctggtatct gcgcagtgaa gaacgttatc gtgctgaagt
     4501 ccccctgtac gcctccatgc tgacacaggc aatggactat gaccggattc tggcaggctc
     4561 acgcagccat cagaccggtg taaacggtga aaataacagc gtccgtctca gcattcaggg
     4621 cggccatctc ggtcacgata acaacggcga tattgcccgt ggggccacgc cggaaagcag
     4681 cggcagctat ggcttcgtcc gcctggaggg tgacctgctc agaacagagg tcgccggtat
     4741 gtcactgacg acaggagtgt atggtgctgc aggccattct tccgttgatg ttaaggatga
     4801 tgacggctcc cgcgccggca cggtccggga tgatgccggc agcctgggcg gatatatgaa
     4861 cctgacacac acctcctccg gcctgtgggc tgacattgtg gcacagggaa cccgccacag
     4921 tatgaaagcg tcatcgggca ataacgactt ccgcgcacgg ggctggggct ggctgggctc
     4981 actggaaacc ggtctgccct tcagtatcac tgacaacctg atgctggagc cacggctgca
     5041 gtacacctgg caggggcttt cgctggatga cggtaaggac aacgccggtt atgtgaagtt
     5101 cgggcatggc agtgcacaac atgtgcgtgc cggtttccgt ctgggcagcc acaacgatat
     5161 gacctttggt gaaggcacct catcccgtgc ccccctgcgt gacagtgcaa aacacagtgt
     5221 gcgtgaactg ccggtgaact ggtgggtaca gccttccgtt atccgcacct tcagctcccg
     5281 gggtgatatg cgtgtgggga cctccaccgc aggcagcggg atgacgttct ctccctcaca
     5341 gaatggcact tcactggatc tgcaggccgg actggaagcc cgtgtccggg aaaatatcac
     5401 cctgggcgtt caggccggtt atgcccacag catcaacggc agcagcgctg aaggttatag
     5461 cagtcaggcc acgctgaatg tgaccttctg ataat
//
LOCUS       DAZFFE010000186         5399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFFE010000186 DAZFFE010000000
VERSION     DAZFFE010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.187"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(158..875)
                     /locus_tag="ACHJ69_004634"
                     /pseudo
     CDS             complement(158..875)
                     /locus_tag="ACHJ69_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1061..1903
                     /locus_tag="ACHJ69_004635"
     CDS             1061..1903
                     /locus_tag="ACHJ69_004635"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO5017447.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKLTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            1906..2994
                     /gene="capU"
                     /locus_tag="ACHJ69_004636"
     CDS             1906..2994
                     /gene="capU"
                     /locus_tag="ACHJ69_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFO5017448.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVVATQIGGIPEVIQNNQTGTLLPAGNKHAWMCALNNFFNDPGRFYQM
                     ARLAKQDIEERFDINKTALKILTLAKQK"
     gene            2999..3949
                     /gene="virK"
                     /locus_tag="ACHJ69_004637"
     CDS             2999..3949
                     /gene="virK"
                     /locus_tag="ACHJ69_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFO5017449.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            4014..4958
                     /gene="lpxM"
                     /locus_tag="ACHJ69_004638"
                     /gene_synonym="msbB"
     CDS             4014..4958
                     /gene="lpxM"
                     /locus_tag="ACHJ69_004638"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO5017450.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKNDTEREIIVDKMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKMI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
     gene            5139..>5399
                     /locus_tag="ACHJ69_004639"
     CDS             5139..>5399
                     /locus_tag="ACHJ69_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5017451.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPSSRTVAELESEILQLRKALNEARLERDILKKA"
BASE COUNT         1506 a         1089 c         1228 g         1576 t
ORIGIN      
        1 accctgcggg catgccgcag gtcactgccg ctcaggttct ccaccgtcac acccggtggg
       61 attgttgggt ttctcatcgt gagttaccgg ttcaatattc cagacagact cgcggttcat
      121 ttaagcatcc atgcccgccc tgaactccgg gcacactata ggtcacatca ccgcaccaca
      181 cctgatttgg ttccgttacg gcgaactgtc gctcaagatg attcgggata gcaacgtgct
      241 catgaccgcc acgcttatac cggtgagtcg gctgctggca actgaccagc cccagctctt
      301 tcatgagtct gccagcaagc cagtgcccca tctggtagcc tctctgggtt gccattgtgg
      361 cgatgcttct tgctccggca gagccgtggc tgatgccatg cagttcaagt acctggctac
      421 gtaatacagc ccgtctgccg tctggttttt caggacggtt tttccagtat ttttagctgc
      481 tgcgatggac cccgaacaca tggcagagag tggccacagg ataacgcgcc ctgagtttcc
      541 cgattatcga aaactgttca gggagtctga catcaagagc gcggtagcct ttttaatatt
      601 tcattttcca tttcaatacg ttgtagcttt ttcctgagct cacggatttc aatttgttcc
      661 ggggtaatgg gggaggtttt tggtgttttt ccctgccgct catcacgtaa ttgtttcacc
      721 catcgcgtca ttgtggaaag gccgacatcc atagcgctgg ctgcatctgc cacggtgtat
      781 ttctggtcaa cgaccaattg agcggattcg cgtttaaact ctgcgctgaa atttcttttt
      841 tcattatggc acctgtgttg ttctgaggtg agcatatcac ctctgttcag gtagctaaat
      901 ttagtgtgcc acctcaaggt tcttagtgag ccgtttttaa aagatatgat aatatattat
      961 tgtttataaa ttgttgatgt ttgatttagt agtatataaa gttcaccaac tttattaggc
     1021 tttcctttta tctctactta ctgggtgcca gaaactttaa attttaaacg gagggggcat
     1081 tttgtttaaa gcaaatcatg ttccggtatt aatgtatcat catgtcagcc attgcccagg
     1141 actggttaca ctttctcccg agacattccg gaaacaaatg aaatggttgg ctgaaaacaa
     1201 ctggaaaaca ctgtcatcag atgaactgga gtttttttat cggggtggaa aacttccccg
     1261 taaaagcgtt atgttgacat ttgatgatgg ataccttgac aactggtttc aggtatatcc
     1321 tttattaaac gagtttaatc tcaaggcaca tattttttta attaccagtt ttattggaaa
     1381 tggtccggtt cgtcactctc ctggaaagga atattctcat cgcgattgcg aacatcaaat
     1441 agcaaccggg aatgctgata atgttatgct tcgctggtct gaagtgaacg aaatgttgca
     1501 atctggtctg gtcgaatttc atgttcatac acatactcat acacgttggg ataaaaagct
     1561 cacttcacga gaagaacagt gtaaacatct tcggcaggac ttattgagcg ggagagaata
     1621 tttaaaaaaa atgacaggaa aatgcagtaa acatttgtgt tggccggaag gttattataa
     1681 caaggattat attcagatcg ctgaagaact tggattccat tatttatata caacagaaag
     1741 aagaatgaat gctcctgcta aaggaactac acggatagga agaataagca ccaaggaacg
     1801 ggaaaattgc gtatggctga aaagacgact tttttattat acaacaccgt ttttttcatc
     1861 gttgctggcc cttcataagg ggccaagatt accggatgac taataatgaa tatactattt
     1921 acggaatcat caccgaatat aggtggtcag gaattacagg ctgttgctca aatgaaggcc
     1981 ctgaagaaaa tggggcattc agttctgctt gtctgcaggg agaacagcaa aattgctttt
     2041 gaagccagta agtttggaat tgatatcaca ttcgcgttat ttcgaaacag tcttcacatt
     2101 cctactgtat ggagattact cggaatagtt catagttttc agcccgatgc aatcgtttgt
     2161 cacagtggac atgatagtaa tattgttggt ttggtacggt tttttacctg gaaacatcca
     2221 ttcagaatta tcaggcaaaa gacatatttg acacgaaaaa caaaattttt ttcaataaat
     2281 catttttgcg atgaggtgat tgttcccgga acaaatatga agacacatct ggagcaggaa
     2341 ggatgtcgaa ctcgggttac tgttgtgcct cctggctttg atttccagga attatacgtt
     2401 gattcccgaa acagtttgcc tccgagtgtt ctttcctggc tggcatcccg aagaggatgc
     2461 cccgtaattg ctcaggtagg aatgttgcgc ccggaaaaag ggcacgaatt tatgttgaat
     2521 ttactgttcc atttaaaaat gaatggacga cagttctgtt ggttgattgt ggggtctggt
     2581 tcacctgaac tgcgggaaca tttacagtat cagattgaca gtatgggcat gcatgatgat
     2641 gtttttattg ctgacaatgt ttttcctgcc gcccccgtat atcgggttgc cagtctggtg
     2701 gttctgcctt cagaaaacga atcttttggt atggtgctgg cagaagcatc ggcattttct
     2761 gtgcctgtag tggccactca gattggtgga atccctgagg ttattcagaa caaccagacc
     2821 gggacattgt taccagcagg taataagcac gcatggatgt gcgccctgaa taattttttt
     2881 aatgaccctg ggcgttttta tcagatggct cgcctggcaa aacaggatat agaagagcgg
     2941 tttgatatta ataaaactgc gttaaaaata ctcacattag cgaagcaaaa gtaacaatat
     3001 gttttctata agtaacttat catttatcgg tttccttaaa aggattattt tttcctcaga
     3061 ttcactaccg gggaagtggg aacacagaaa atttcggttc atgtacattt tgcgatgttc
     3121 tataaatccg gttgtcagta ttcgatatta ttatgaactg cgttccttgc cgtgcattga
     3181 ggatattctg gcaatacacc ccacgttgcc agcaagaatt catcgccctt atttgcataa
     3241 aggggggcgc gcctggaccc gtggacaata tattcttgag cattatcgct ttgtgcaaaa
     3301 tttgccagaa aaatattcta aatttttgtt tccacaaaaa tcggtatcat tagtccaatt
     3361 catcgggaaa gatggagaga attttgatat ccagtgttct cccagtggtt ttgaccggga
     3421 aggagagttg atgttgtcat tgttttacaa taaaacagtt attgcccggt taacattttc
     3481 agtcattctg actcaaaatg gacacattgc ttttattggc ggattacagg gagcccctaa
     3541 aaataccgga cctgatgtta ttcggtgcgc aacccgggcc tgttatggga ttttcccaaa
     3601 acggattatt tttgaagcat tttgtgcgtt gatgaaagct tgcaatatct ctgagtgtct
     3661 ggcagtcagt gagcacagtc atgttttccg gcaattgaga tactggtatc agaaacgcaa
     3721 aacctttgtt gcggtctata gtgatttctg ggagtctgta gcaggaaaaa cctgtggtga
     3781 ctggtacaga ttaccaaccc aggtgattcg aaagccgcta agcgatatag ccagtaaaaa
     3841 acgctctgag taccgaaaac gatacgcttt gctggattat atccatgaaa ctactatccg
     3901 ctctttggat gcatatcctg tacactcaga acatcagaat ttaaattaag gtttgatgta
     3961 tcctctgaat aaaacagtaa tgataatttt ggtagaggca ttcgcactaa ataatgaaaa
     4021 aatataaatc tgagtttatt cctgaattta agaaaaatta cctttcccct gtttactggt
     4081 ctacatggtt ccttttggga atgattgcag gtatttcaat gtttccccct tcattcagag
     4141 atcctgtctt ggcaaaaata gggcgttggg cgggcagatt gagcaaaaaa gctcgtcgca
     4201 gggcgacgat taatttatcg ctttgtttcc cagaaaagaa tgatacagaa cgggaaataa
     4261 ttgtcgacaa aatgtttgcc acagcattgc aatccatagt gatgatggca gaactggcaa
     4321 ttcgtggtcc ggaaaagttc cagaaacgag tgttctggaa aggcctcgaa attcttgagg
     4381 aaattcgaca caataataga aatgtgattt ttctggttcc ccatggctgg agcgtggata
     4441 ttcctgcaat gttgctggca gctcagggaa aaaaaatggc cgccatgttt catcagcaac
     4501 gaaatccagt gattgattat gtctggaatt cagtacggcg taaattcggg gggcgcttac
     4561 atgcccggga ggatgggata aaaccattta ttcagtcagt acgccaggga tactggggat
     4621 attacctccc agatcaggat catggtcctg aatacagtga atttgctgat ttttttgcga
     4681 cctataaagc gacattacca atcattggac gtctgatgaa catcagtcag gctatgatta
     4741 taccactttt cccggtttat gatgaaaaaa aacatctcct gactattgaa attcggccac
     4801 caatggatgc ttgcattgcc agcgcggaca ataaaatgat tgcccgacaa atgaacaaaa
     4861 cagtggaaat tttggtgggg ccacatccgg aacagtatgt ctgggtttta aaattgttaa
     4921 aaacacgcaa atcaaacgaa gcggacccgt acccttgaaa ttataaagta taagtatttc
     4981 cactagttgt ttgtaagcgt acagcctgaa ccgtctggtc agaatctgac gaattagaca
     5041 aagtggtgtc caccaaataa gtagtgggaa ccaatgaggt agcctgagtt taacggacac
     5101 tccttcctga aatagaatgg catcagaagg agctaataat gagcagaaaa acccaacgtt
     5161 actctaaaga gttcaaagcc gaagctgtca gaacggttct tgaaaatcaa ctttcgatca
     5221 gtgaaggcgc ttcccgatta tctcttcctg aaggcacttt aggacaatgg gttaccgccg
     5281 ccagaaaagg gctcggtact cctagttccc gcacggtggc tgaactggaa tctgaaattc
     5341 tgcaactgcg taaggcgtta aatgaagctc gccttgagcg agatatatta aaaaaagca
//
LOCUS       DAZFFE010000187         5365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFFE010000187 DAZFFE010000000
VERSION     DAZFFE010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.188"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(112..1599)
                     /gene="yeeO"
                     /locus_tag="ACHJ69_004640"
     CDS             complement(112..1599)
                     /gene="yeeO"
                     /locus_tag="ACHJ69_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO5017452.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAVAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGLMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
     gene            1760..1835
                     /locus_tag="ACHJ69_004641"
     tRNA            1760..1835
                     /locus_tag="ACHJ69_004641"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1793..1795,aa:Asn,seq:gtt)
     gene            complement(1873..2823)
                     /gene="cbl"
                     /locus_tag="ACHJ69_004642"
     CDS             complement(1873..2823)
                     /gene="cbl"
                     /locus_tag="ACHJ69_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO5017453.1"
                     /translation="MNFQQLKIIREVARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQISPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2925..3842)
                     /gene="nac"
                     /locus_tag="ACHJ69_004643"
     CDS             complement(2925..3842)
                     /gene="nac"
                     /locus_tag="ACHJ69_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO5017454.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLNRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4169..4244
                     /locus_tag="ACHJ69_004644"
     tRNA            4169..4244
                     /locus_tag="ACHJ69_004644"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4202..4204,aa:Asn,seq:gtt)
     gene            complement(4300..5235)
                     /gene="ldtA"
                     /locus_tag="ACHJ69_004645"
     CDS             complement(4300..5235)
                     /gene="ldtA"
                     /locus_tag="ACHJ69_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO5017455.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPDTVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWLTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5297..>5365)
                     /locus_tag="ACHJ69_004646"
     CDS             complement(5297..>5365)
                     /locus_tag="ACHJ69_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017456.1"
                     /translation="NMGELAASNIVLPGNTTSDLNS"
BASE COUNT         1508 a         1394 c         1159 g         1304 t
ORIGIN      
        1 actacagagg aatcgtgaga acgaggcgaa tattagcgat gcccacccac aatgtcaaag
       61 cctgtttttt aaatttgaaa tcgtttgctg aaataatctg cattttgtcg tttattccga
      121 cacaactggc tttttttcac acttttgcgg ctcgggtcga gggtatttcc atagccaacg
      181 tccagtaacc attcgccagt aaaacagcac ggcccgcaca gcccagtcgg caaacattcc
      241 catccagaca ccaaccacac cccagccaag catgattccc agcacataac cgactacaac
      301 ccgacaaccc cacatgctca acatcgaaac ccacatagcg taacgggcat cacgagcacc
      361 tttaaatcca gcgggtagca cccatgaggc ggaccaaata ggcataaata aagcatttag
      421 ccaaatcaga atcacaacga catgtttaac ctgtggatcc tgggtgtaaa acgatgccat
      481 aagcccggca aagggagccg ttagccaggc gatggccgtt aatccaagag tggacagcca
      541 gaacacatgc cgcaactgaa tctctgcttg cgctatctgc cctaccccca accttcggcc
      601 tgtaatgatc gtagaagcag agccgagcgc acttccgggt aagttgataa gagccgcaat
      661 tgaaaacgcg ataaaatttc cggcaataac actggtcccc atcccggcaa cgaacatttg
      721 ggttaataac cgaccactgg taaataacac tgattcgaca ctagcgggaa taccaatccc
      781 catgacttcc cagataatgc taaaattcag cggtttaaaa tagctcttta acgaaatcct
      841 tagcgcagga ttaaaaccaa tcgctagcac ccacaaaatt gcaactgcgc caatataacg
      901 agaaatggtt aaacccagcc ctgccccgac aaatcccagt cccggccagg agaaaaggcc
      961 gtaaatcaat atgccgctaa taataatatt aagaatattc aggctaccgt taatcagtag
     1021 cggtattttc gtattccctg caccacgaag tgccccgcta ccaatcaaag tgatggcaac
     1081 tgctggataa ctgagtaccg tcagctccag ataagtcaac gccagtgctt taacttctgt
     1141 cgtggcatca cccgcgacga aatcaataat ttgttcgcca aaatgatgaa taagcgttgc
     1201 caacagtacg gcaaacaacg tcatgatcac caatgactgc cgcgtcgcca ccctcgctcg
     1261 tcgtcgatcc cgcttaccga gactaaatgc cacaacgaca gtagtaccaa gatcgatagc
     1321 agcaaaaaaa gccataatga ccatattgaa gctgtccgcc aatcccacgc cggccatcgc
     1381 atcttttccc agccagctga ccagaaaagt gctcaggaca cccatcaaca ggacacaggc
     1441 attctccatg aagataggaa cagcaagcgg ggttatctcg cgccagaaca acactttgta
     1501 gctcttgcgt ttagcgtgcc agcgagtgcc gtgaacaacc tggcgtaaag cagaggagat
     1561 attcaaagcc gaccttaatt gcagaaagtg aaaccacatt tcaaataatg agggagaatc
     1621 agcaaagctg caaagatttt cgccaacaaa ttgtctgcaa atgcaacaaa ctgttgatag
     1681 aaacggcaaa cagttgggaa atttaaaaat cgggtttgac aaaagatttt tcgccgttaa
     1741 gatgtgcctc aacagcgatt cctctgtagt tcagtcggta gaacggcgga ctgttaatcc
     1801 gtatgtcact ggttcgagtc cagtcagagg agccaaattt ctgaaaagcc cgcttttata
     1861 gcgggctttt tgctatatct gataatcaat ttcctcttca ctgttctcca tcacctggcg
     1921 cttgatatcc tcaactgaca gtcctgcatt acaaagttcc agaaagcgcc agacatagtt
     1981 acgctgaagt tgccctcgct tcagtcccaa ccatacagta ttggcatcga aaagatgccg
     2041 cgtatccaga cggattaaat tctcttcctc ttgttcgcca ctggattgct cggcaactaa
     2101 tccgatccca agcccaagag caacataggt tttaatgaca tcagaatcct gcgcacttaa
     2161 tacaatatct gccagcaaac ctttgcgggc aaatgcgtca tcaatacgtg agcgccccgt
     2221 aatcccctgt cggtaagtga ttaacggcca cttcgctatt gattccagcg tcaatggtga
     2281 aatttgcgtc aagggatgat cgtgtggaac aagcaaacta tggtgccaac gaaaccacgg
     2341 gaaggcgacg agctgcgggt cattactcaa acgctcgctg gcgataccaa tatcagcttc
     2401 gccattttgc aacaatgtcg caatttcctg tggcgtcccc tggattagct cgagccgaac
     2461 ctccgggaaa agttcgcgaa aagctttaat gacctctggc aagctataac gtgcctgagt
     2521 atgcgtcgtt gcaatagtga gaacgccaga cgtatcgttg gtaaacaggt ctgcaagccg
     2581 acgaacatta ctggcttcat tcagaatacg ttctgcaatg accagtaatg ctttgcccgg
     2641 ttcagtcatg cccagcagtc gcttacctcg tcgaacaaat atttcgatgc caagttcatc
     2701 ctccagttcc cgaatatgac ggctgacgcc tgactgtgag gtaaaaagca tattcgcaac
     2761 ctctgtcagg ttgtaatcct gacgtgcaac ctcgcggatt atctttagtt gttggaaatt
     2821 cacggtaaac tccgggcagt tcagatttcc cgttattgtt aaagtctaat gcccggcata
     2881 acaaataata aaaacccgca tcttattcca tcccgatata acacttagct caccaattgc
     2941 cactgccttt tttccatcac tggagaacta atcactgaca ttaacaactc tttcactgcc
     3001 tgtgcctgtg gcgataagtt cgctctggcg ggtaaattta atgacaaaga gagactcatg
     3061 gaaggagtgg taatgcgtga catccaccca tttactgcgc cacataacga acgcgcggcc
     3121 gattcgggta atactgcaac gcccatgccg ctggcaatcg ctgcggtaag cgtggcaata
     3181 gactcaattt caccaataac ttttgccgtg agtcgccgta gggaaaaagc ctcatcaaca
     3241 cgaagtctaa tagcactgta atcactgggg agaaagaggt tcatttgcgc aatagcattc
     3301 acatcaacgc tttgccccgg gcaatcttga gttcctacca gaaaaagatc ttctttcagc
     3361 aaagcctgac tggatacacc agccacaggg gaatgctcat aaatcaccgc catatcgagt
     3421 tggtgattta tcaatttttc gttaagcact gcaccactat tttcatgaag atagataacg
     3481 atctccggaa attcagcgcg aaccgcctgt aataagggca tggtgatgga tgacgcagcg
     3541 gttcctggtg caaagccaat cgagacttgc cccgataatg cctgaccaac gttatgcacc
     3601 gccagttggg cctgttcaca ctgacgtaaa atggcccgcg catgggtata gagaattttt
     3661 ccggcgtctg ttggtgtaac gccccgcttt gtacggttca aaagttgttg atttaactca
     3721 ccttccagtg tggcaacctg ctggctgagc gctggttgtg cgatatgcaa tacttcagca
     3781 gcctgggtca ggctaccaat atctacaatt tttacgaagt atttcaggcg tctgaagttc
     3841 atgttgcctc cgttttttaa gaatcggccc aagtgccgcc attacttaca accagattgc
     3901 aagatgcttg ccagttttat tttggtgttg atgtacaagc taaccaactg tcaaataaga
     3961 gattatgata gattcgtcat ttgctccttt aatcagctgt cgcgttcccc tgccctataa
     4021 aaggggggta tgcaccacga tggttcatta cccaataaga ttgaaagctc accactttgt
     4081 tgaaattgac agcaaacaaa caaaaaaatg catttcaccc tttgacatca ccatgcactg
     4141 ccattaatat gcgccccgtt cacacgattc ctctgtagtt cagtcggtag aacggcggac
     4201 tgttaatccg tatgtcactg gttcgagtcc agtcagagga gccaaattca aaaaagcctg
     4261 ctttctagca ggctttttgc tttctaatta ccaacgctct taaaacatct gtcttgaacc
     4321 agaactaatt tgcacaggca ttcccgatcg acgttgcaac gcagcatttg cgcgatttac
     4381 atcaacttct tgcccgttga taaacgcccg caaagatggg gttaccggca atggcacttt
     4441 tcggtcagac tcatattctg cacgattgcg cgacaacggc tcatgaactt ccagccagtt
     4501 cgagccatct ggttcagtgg tgtattttac tggctggtcg ataatttgca cacgcgtccc
     4561 aacaggaaca ttatcaaaca gatatttgat atcgtcattg cgcagacgaa tacagccctg
     4621 acttacccgg agcccaatac caaaattggc attggtacca tggatggcat acaacctgcc
     4681 aatataaatc gcgtacagcc ccatgggatt atcggggccc gcaggaacaa atgcgggcaa
     4741 actctcccct cgtttcgcat attcgcgccg agtgttcggc gttggcgtcc aggttggagc
     4801 ttcttgttta cgttcaacgg tagtcaacca gttacgcggg gtttctcgcc cagcctggcc
     4861 gataccaata ggaaagactt ccacagtatt actgtctggt gggtagtaat aaagacgcat
     4921 ctcagcgacg ttaacaacaa tccctttacg aacagtgtcg ggcaaaatca gttgctgcgg
     4981 aatggtgagt tgcgagccag acttcggcaa aaaaacatca gcgcccgggt tcgcttccag
     5041 catgttactt aacccttgcc cgtattgtgc ggcaaaagtc tccagcggct gggtattgtg
     5101 atcaggaaca gttacagtaa gcgactgccc caccaaacgg ctaccctctg gaggtaatgg
     5161 ataagttacc gccaggctag tatggctggc aaaaagcaga gcaaatgagc aaagaatatt
     5221 tacacgacgc atcatgtccc tttcctatgt cgcgaaagct atccgttaag tatagctttt
     5281 atcagacttt tcgtttttaa ctgttcaaat cagaagtcgt attccccggt agaacaatat
     5341 tactggcagc aagttcgccc atgtt
//
LOCUS       DAZFFE010000188         5349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFFE010000188 DAZFFE010000000
VERSION     DAZFFE010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.189"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            50..1846
                     /locus_tag="ACHJ69_004647"
     CDS             50..1846
                     /locus_tag="ACHJ69_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HFO5017457.1"
                     /translation="MFDKYRKTLVAGTVAITLGLSASGVMAAGFKPAPPAGQLGAVIV
                     DPYGNAPLTALVDLDSHVISDVKVTVHGKGEKGVEISYPVGQESLKTYDGVPIFGLYQ
                     KFANKVTVEWKENGKAMKDDYVVQTSAIVNNYMDNRSISDLQQTKVVKVAPGFEDRLY
                     LVNTHTFTAQGSDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLD
                     QDTFYDGRDRDINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFAD
                     ATHESIETPNGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDP
                     KRDALLGALDAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAK
                     DDSIILSSRHQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENGL
                     CENSDFDFTYTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGT
                     VQQVWEYGKERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTVGKLNEIDYK
                     TKEVKVEIDVLSDKPNQTHYRALLVRPQQMFK"
     gene            1864..2532
                     /locus_tag="ACHJ69_004648"
     CDS             1864..2532
                     /locus_tag="ACHJ69_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFO5017458.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDSKDPAAFIKTGLDAAGMSQA
                     DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRE
                     LASK"
     gene            2547..3218
                     /gene="dsbI"
                     /locus_tag="ACHJ69_004649"
                     /pseudo
     CDS             2547..3218
                     /gene="dsbI"
                     /locus_tag="ACHJ69_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
     gene            complement(3264..4052)
                     /gene="ygiD"
                     /locus_tag="ACHJ69_004650"
     CDS             complement(3264..4052)
                     /gene="ygiD"
                     /locus_tag="ACHJ69_004650"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO5017459.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMEMPPTIHDFGGFPQALYDTYYPAPGSPALAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            4195..4968
                     /gene="zupT"
                     /locus_tag="ACHJ69_004651"
     CDS             4195..4968
                     /gene="zupT"
                     /locus_tag="ACHJ69_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO5017460.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTVGIG"
     gene            complement(5030..5200)
                     /locus_tag="ACHJ69_004652"
     CDS             complement(5030..5200)
                     /locus_tag="ACHJ69_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017461.1"
                     /translation="MFIAWYWIVLIVLLAGGYFLHLKRYCKAFRHDRDALLAARSKLD
                     KRSDKEVFEKHQ"
BASE COUNT         1397 a         1299 c         1290 g         1363 t
ORIGIN      
        1 tcgatattca tcattattat atatttgcgt tatataagga gatatatcca tgtttgataa
       61 atatagaaaa acactcgtag ccggaactgt ggcgataacc ctgggtttgt cagcatcggg
      121 ggtgatggct gcgggtttta aaccagcgcc acctgccggg caactgggtg cggtcattgt
      181 cgatccctac ggcaatgcac cactgaccgc tttggttgac ttagatagcc atgttatttc
      241 tgacgtcaaa gttaccgtcc atgggaaggg cgaaaaaggc gtagaaatca gctatcccgt
      301 gggtcaggaa tcactaaaaa cttacgatgg tgtaccgatt tttggtcttt atcagaaatt
      361 tgctaacaaa gtgaccgttg agtggaaaga aaacggcaag gccatgaaag atgattacgt
      421 ggtgcagact tccgccatcg tcaacaatta catggataac cgctccatct ccgacctaca
      481 gcaaaccaaa gtcgttaaag tcgcgccagg ttttgaagat cgcctctatc tggttaatac
      541 ccacaccttt accgcccaag gttccgatct ccactggcat ggtgagaaag ataaaaatgc
      601 cggtatcctt gatgcgggtc cggcaactgg cgcactccct tttgatatcg cgccattcac
      661 ctttatcgtc gataccgaag gtgaataccg ctggtggctg gatcaagaca ctttctacga
      721 tggtcgtgac cgcgatatca acaagcgtgg ttatctgatg ggtatccgcg aaacgccacg
      781 cggcaccttt accgctgtac aaggtcagca ctggtacgag ttcgacatga tgggacaggt
      841 gctcgaagat cacaaactac cgcgcggatt tgctgacgcc actcatgagt ccattgagac
      901 gccaaatggc acggtactgt tgcgcgtagg taagagtaac tatcgtcgcg atgacggcgt
      961 acacgtcacc accattcgtg accatatcct cgaagtcgat aaatctggtc gcgttgtcga
     1021 tgtatgggat ctgaccaaga tcctcgatcc gaaacgcgat gcactgctcg gcgcgctgga
     1081 tgcaggtgca gtttgcgtta acgttgacct tgcccatgca ggacaacagg caaaactgga
     1141 accagataca ccgttcggcg acgcgctggg tgtagggcca ggccgcaact gggcgcacgt
     1201 gaactctatc gcctatgacg caaaagatga ctctatcatc ctctcctctc gccaccaggg
     1261 tgttgtgaag attggtcgtg ataagcaagt gaaatggatc cttgcgccct ctaaaggttg
     1321 ggaaaaaccg ctggccagca agctgctgaa accggtggac gctaacggta aacctattac
     1381 ctgtaacgaa aacggtctgt gtgaaaactc ggacttcgat tttacctaca cccagcatac
     1441 cgcctggatt tccagcaaag gaacgctcac catttttgat aatggcgatg gtcgtcatct
     1501 ggaacaacct gccttaccaa ccatgaaata ttcccgcttt gtggaatata agattgatga
     1561 gaagaaaggc accgttcagc aagtgtggga atacggtaaa gaacgtggct acgatttcta
     1621 tagcccgatt acctccatca ttgaatatca agccgaccgt aacaccatgt ttggcttcgg
     1681 tggttctatt catttgttcg atgtcgggca gccaaccgtc ggtaagttga acgaaatcga
     1741 ttacaaaacc aaagaagtga aagtggaaat cgacgtgctg tcagataaac ccaatcagac
     1801 tcactaccgt gcgctgttag tccgtccaca acagatgttc aaataattaa gaagggaata
     1861 aaaatgtcga aattagggat ctcatcacta tttaaaacca tactgcttac cgcagctttg
     1921 gctgtttcat ttaccgcttc tgcatttacc gaaggcacgg attacatggt tctggaaaaa
     1981 cctattccga atgccgacaa aacgctgatt aaggtattta gctacgcctg cccgttctgt
     2041 tacaagtacg acaaagcggt taccggtccg gtatcggaaa aagtgaaaga tatcgttgct
     2101 ttcacgccgt tccatctgga aacaaaaggt gaatacggca aacaagcaag cgaagtcttt
     2161 gcggtcttga tcaataaaga taaagcagca ggcatttcct tatttgatgc taactctcag
     2221 tttaagaaag ccaagtttgc ctactacgcg gcttatcacg acaaaaaaga acgctggtct
     2281 gacagtaaag atccggctgc ttttatcaaa accggtctgg atgccgcagg tatgagtcag
     2341 gcggattttg aagcggcact gaaagaacca gcggttcaag aaacactgga aaaatggaaa
     2401 gcctcttatg atgtcgccaa aatccagggg gttccagcct atgtcgttaa tggtaagtac
     2461 ttgatctaca ccaagagcat caaatccatc gacgcgatgg cagaccttat ccgtgagtta
     2521 gcaagcaaat aacagggaaa caggctatgg gtattaaggg aatgtggaaa gatcttcgca
     2581 catcaccagt tgacacactg gtcagatggc aggagcaacg cttgttgtgg ttgttaatgg
     2641 ccgttgcgat gggcgcgtta attatcctgg cgcactcttt cttccagatc tatctctaca
     2701 tggcaccctg tgagcaatgt gtatacattc gctacgccat gtttgtgatg gttattggag
     2761 gattggttgc agcgatcaac ccaaagaaca tcatcctaaa gctaattggc tgcgtgatgg
     2821 cattttacgg cagcattttg ggactgaagt tttcgctcaa actgaacgat atccaccatg
     2881 cagtacataa tccggatccc gattaactgt ttggcgtaca gggttgctct accgatccaa
     2941 cttttccctt taacctgccg ctggcacagt gggcaccaaa ttggttcaag cctaccgggg
     3001 attgtggcta tgacgcacca atcgttcctg atggcgtcac actgagcagc acgcaacagt
     3061 ggtttgtgga aatgtatcag caatctgaag gctggtatct gctaccacca tggcatttta
     3121 tgagtatggc tcaggcttgt atgctggctt ttggtatgtg tctggtgctg ctggtcatta
     3181 tgagtgacgc atgggcgctt aaaatcatca gaggctaact cagcaacacc cgaagccagt
     3241 cattctggct tcgggttctt ttattaacct atctgtaccg acagcatact taaactgccc
     3301 atttctatac catcgaccgg aatggtaatt ggctcctgcc catcccacgc ccctaagaca
     3361 tacaacaacg gcagatagtg ctctggcgtt gggttcgata acgcgccacc ttcatggtcg
     3421 agataattca ccagaggatg ttgttccacc ggcccttgcc acgtcagatt cgctttcaca
     3481 tactcattaa acgacgtcgc ccacggatac ggtgaactat caccgtgcca cttcactgta
     3541 cgcaggttat gtaccacgtt gccactggcg accaacatta tcccttcatc acgcagcgcc
     3601 gcgagtttgc gccccatttc gaaatgccag gcggcaggtt tgctactgtc gatactcaac
     3661 tgcaccatcg ggatatcggc gtcagggtac atcttaatca gtacgcccca cgagccgtgg
     3721 tcgaagcccc aggcttcttt atccagcgtc acagggaccg gcgctaacag ctcaaccaga
     3781 tgctgtgcca gcgcaggtga acccggagca ggataatacg tatcgtacag cgcctgcggg
     3841 aagccaccaa agtcatgaat cgtgggcggc atctccatcg cggtcactcc tgttccacgg
     3901 gtaaaccaat gagccgaaac caccactatt gcttgcgggc gtggcaatgt catccccaac
     3961 gtctgccagc tgcgggtata caaattatct tccagcacgt tcatcggact accgtgacct
     4021 aaaaacaatg ctggcatacg tgttgaagac atgatgatat ccttaactaa aggtgtcatt
     4081 ttgatatcct cacaatacgc ttgttcggcg gagtaagaac ccggataaca atgatgatga
     4141 tcatcagtta ttttgacgat ctgcctgaag gtaaagattt ataaggagtt gtcgatgtca
     4201 gtacctctca ttctgaccat actggcgggg gcagccacgt ttattggcgc gtttctcggc
     4261 gttctcgggc aaaaaccctc gaaccgctta ctggcgtttt cgctaggatt tgcggcgggg
     4321 atcatgttgc tcatctcatt aatggaaatg cttcctgccg cattagccgc cgaaggaatg
     4381 tcgcctgtgt tgggttatgg aatgtttatc ttcggtctgc ttggctattt tggcctggac
     4441 cgcatgttgc cacatgcgca tccgcaggat ttaatgcaaa aatcggtgca gccgttgcca
     4501 aaatcgatca agcgcacagc cattctgctc actctcggca tcagtctgca taacttcccg
     4561 gaagggattg ccacctttgt cacggcgagc agcaacctgg agctgggatt tggcatcgcc
     4621 ctggccgtag cgttgcacaa tattcctgaa ggtctggcgg ttgcaggccc ggtttacgct
     4681 gcaacaggat cgaaacgtac cgcgattctg tgggcgggca tttccgggct ggcagaaatt
     4741 cttggtggtg tgctggcatg gttaatcctc ggtagcatga tttctccggt ggtcatggcg
     4801 gcaatcatgg cggcggttgc aggaattatg gtggcgctct ccgttgacga attaatgccg
     4861 ctcgccaaag agattgaccc caataataac cccagctatg gcgtactatg tggaatgtca
     4921 gtgatgggat tcagtttagt gctgctacaa acggtgggaa ttggttaaag acgataatga
     4981 cagatgttgc ccattgctaa aggcaacatc tgtatcgtta ttaaaggtat tattgatgct
     5041 tttcaaacac ttctttatcg gagcgtttat ctaatttact tcgggcagct aacagcgcat
     5101 ctctgtcatg gcgaaaagct ttacagtaac gtttaagatg aagaaaatag ccacccgcca
     5161 gtagaacaat taatacaatc cagtaccagg caataaacat ccttttcacc ttcaaaattt
     5221 aacattacta aacatttagc gttaaaattt cacatatcca tcgttgaata cattcatata
     5281 ctcgcataat tctggatcaa gtatatgatt tcgatcatag taagtgttgt attatatacc
     5341 atatctggt
//
LOCUS       DAZFFE010000189         5339 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFFE010000189 DAZFFE010000000
VERSION     DAZFFE010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.190"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(330..1220)
                     /gene="rarD"
                     /locus_tag="ACHJ69_004653"
     CDS             complement(330..1220)
                     /gene="rarD"
                     /locus_tag="ACHJ69_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO5017462.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(1272..1739)
                     /gene="yigI"
                     /locus_tag="ACHJ69_004654"
     CDS             complement(1272..1739)
                     /gene="yigI"
                     /locus_tag="ACHJ69_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO5017463.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNKPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1904..2773
                     /gene="pldA"
                     /locus_tag="ACHJ69_004655"
     CDS             1904..2773
                     /gene="pldA"
                     /locus_tag="ACHJ69_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO5017464.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYIIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSDESSPFRETNYEPQLFLGFATDYNFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2898..4727
                     /gene="recQ"
                     /locus_tag="ACHJ69_004656"
     CDS             2898..4727
                     /gene="recQ"
                     /locus_tag="ACHJ69_004656"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO5017465.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQISLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4791..>5339
                     /gene="rhtC"
                     /locus_tag="ACHJ69_004657"
     CDS             4791..>5339
                     /gene="rhtC"
                     /locus_tag="ACHJ69_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO5017466.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRL"
BASE COUNT         1336 a         1318 c         1373 g         1312 t
ORIGIN      
        1 agcctaatat gttgttcatt tatattctcc tttcttataa ttaagggcaa tgttaatttg
       61 ataagaaata atagggtaaa aaaattttaa aaaagaaatg cacttcacat tttttattac
      121 attctgaaga gaacttggtt aatgattggt cttgctcatc gctaataaca taatgctgaa
      181 tttaccaaaa atgaaattta ttaaagcaaa tgaaaatata caacgacgtc tggaaattct
      241 gcctttcctt atgtgagtct cctgtcagca tggcaatatg cccactctca tgcaagagtc
      301 ggcatatttt ttcagaatat atttattttt tatttggacg ttctacgctg cgtataaatc
      361 gcatccatca caaaaattgc cagcgccacc caaataaagg cgaaagttac catcttatcg
      421 gcacccggtt tttcaccata aaacgtcaca gccagcagga acatcagcgt cggaccaatg
      481 tactggaaaa agcctaacgt tgagagacgc aggcgtgtcg ccgctgcagt aaaacacaac
      541 agtggcacgg tggtgacaat accggcggcg atcagcagta aattcagcga catcgggttt
      601 tgccccatat gactggttga gctgtcggca atagcaaaca ggtaaatcgc cgccacgggc
      661 agcaaccaca tagtttcgat taacatgccg gtttgcgctt caacagcaat cttcttgcgc
      721 accaggccgt agaaagcaaa actaaacgcc agccccagcg cgatgatcgg cagtgaacca
      781 aaagtccaca actgaaccag cactccacat atcgccagaa tcaccgccag ccattgcatt
      841 cggcggaatc gctcgccgag gaaaatcatc cccagcacaa tgttcaccag cgggttaata
      901 aagtaaccaa ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc
      961 cagttgccgc caatcagcac ggcagagact gccagcataa aaattttctg tggcgtctga
     1021 atcagcgttt ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa
     1081 aacgaccaga tcacgcgatg cgtcaggatc tcatcggcgg gcacgtagta aatcagtttg
     1141 aagtacgctg gcgcaatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc
     1201 cgcgtttgtt ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg
     1261 cttttatgtc ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct
     1321 tcattgtgta attcaacgcg ggcgacggcg actttattgc ctgcacgtaa caggctactg
     1381 gtagcagtaa aacgctcgcc tctgcctggg cgcagataat cgacgcgaag atcaatggtc
     1441 cccatccgcg atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag
     1501 gtacttccca cgcacaccag acctgcggcg acatccagcg ccgacgcaat gaccccgccg
     1561 tgtaaaatgc tttgcgccca gttgcccacc atcatcggct tatttttaaa ggccagctgt
     1621 gcgaactctt tttcgtaacg ctccagttcc atccccaatg cgcggttaaa tggcatgtga
     1681 taaacaaaca tctcacccac taatttgagg gcttgttcag cggtcagtac ggcagacata
     1741 acatccttac acttcattag ttaatggaat gttgatttta tgcttattta ttgttggttt
     1801 ctactttagg aagggataac taacggctat ggagttaagt atgtaaaata gcccgcagaa
     1861 aaatattcac cttatcaata attcgttacg gagaacacga gcgatgcgga ctctgcaggg
     1921 ctggttgttg ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa
     1981 agaggtgcat gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca
     2041 tgacaatccg ttcacccttt atccttatga caccaactac atcatctaca cccaaaccag
     2101 cgatctgaat aaagaagcga ttgccagtta cgactgggcg gaaaacgcgc gtaaggatga
     2161 agtaaagttc cagttgagcc tggcgtttcc gctgtggcgt gggattttgg ggccaaactc
     2221 tgtactgggc gcctcctaca cacagaaatc ctggtggcag ctttctaata gcgatgagtc
     2281 atcaccgttt cgtgaaacta actacgaacc gcaattgttc ctcggttttg ccacggatta
     2341 caactttgct ggctggacgc tgcgcgatgt ggaaatgggc tacaaccacg actcaaacgg
     2401 gcgttcagac ccgacttccc gcagctggaa ccgcctttat acccgcctga tggcagaaaa
     2461 cggtaactgg ctggtagagg tgaagccgtg gtatgtggtg gggaatacgg acgataaccc
     2521 ggacatcacc aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc
     2581 ggtactgagc gcgaaagggc agtacaactg gaacaccggt tacggcggcg cggagttagg
     2641 cttaagctac ccgatcacca aacatgtgcg cctttatact caggtttaca gcggttacgg
     2701 cgaatcgctc atcgactata acttcaacca gacccgcgtt ggtgtgggag ttatgctaaa
     2761 cgacctgttt tgatgaatgg ttggcaaatc tggaatccag catccaggat taccctctca
     2821 gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa atagcgcctg tttttatttc
     2881 aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa tctggagtcc ggagctaaac
     2941 aggttttaca agaaaccttt ggctaccaac agtttcgccc cggccaggaa gagattatcg
     3001 acaccgtact ttccggtcgc gattgcctgg tcgtcatgcc cactggtggc ggaaaatccc
     3061 tttgctatca aatccctgcc ttattgctaa acggccttac cgtggttgtc tcaccgctga
     3121 tttcgttgat gaaagatcag gtagatcaac tgcaagccaa cggcgtggcg gcggcgtgcc
     3181 ttaactcgac gcaaacccgc gagcagcagc ttgaagtgat gacaggctgt cgcaccgggc
     3241 aaatcagcct gctttatatc gcgccggaac gcctgatgct ggataacttt cttgagcatc
     3301 tggcgcactg gaatccggtg ttattagccg ttgatgaagc gcactgtatc tcccaatggg
     3361 gtcacgattt ccgcccggaa tacgctgccc tcggtcagct gcgtcagcgt tttccgacgc
     3421 tcccgtttat ggcgctgact gccacagccg atgacaccac gcgccaggat atcgtgcgcc
     3481 tgctggggct gaacgatccg ctgattcaaa tcagcagttt tgaccgtccg aatattcgct
     3541 acatgctgat ggagaaattc aaaccgctcg atcagttgat gcgttatgta caggaacaac
     3601 gcggtaagtc ggggattatt tactgtaata gtcgtgcgaa agtagaagac accgctgcgc
     3661 gcctgcaaag caagggaatt agcgcggcgg cctatcatgc cgggctggaa aataatgttc
     3721 gcgctgatgt gcaggaaaaa ttccagcgcg atgacctgca aattgtggtg gcgacggtgg
     3781 cgttcggcat gggcatcaac aagcctaacg ttcgcttcgt ggtgcatttt gatattccgc
     3841 gcaatatcga atcctattat caggaaactg gacgcgccgg gcgtgatggc ctgcccgcgg
     3901 aagcgatgct gttttacgat ccggctgata tggcgtggct gcgccgttgt ctggaagaga
     3961 agccgcaggg gcagttgcag gatatcgaac gtcataaact caatgcgatg ggcgcgtttg
     4021 ctgaagcgca aacttgccgt cgtttggtat tgctgaacta ttttggcgaa gggcgtcagg
     4081 agccgtgcgg gaactgcgat atctgcctcg atccgccgaa acagtacgac ggttcaaccg
     4141 atgcgcagat tgccctttcc accattggtc gtgtgaatca gcggtttggg atgggttatg
     4201 tggtggaagt gattcgcggt gctaataacc agcgtatccg cgactatggt catgacaaac
     4261 tgaaagtcta tggcatgggc cgtgataaaa gccatgaaca ttgggtgagc gtgatccgcc
     4321 agctgattca cctcggcctg gtgacgcaaa atattgccca gcattctgcc ctacaactga
     4381 cagaggctgc gcgtccggtg ctgcgcggcg aatcctcttt gcaacttgcc gtgccgcgta
     4441 tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg cggcaactat gatcgcaaac
     4501 tgttcgccaa attacgcaaa ctgcgtaaat cgattgccga tgaaagcaat gtcccgccgt
     4561 acgtggtgtt taacgacgca accttgattg agatggcgga gcagatgccg atcaccgcca
     4621 gcgaaatgct cagcgttaac ggcgttggta tgcgcaagct ggaacgcttt ggcaaaccgt
     4681 ttatggcgct gattcgtgcg catgtcgatg gtgacgacga agagtagtca gcagcataaa
     4741 aaagtgccag gatgaagact ccgtaaacgt ttcccccgcg agtctaatgt atgttgatgt
     4801 tattcctcac cgtcgccatg gtgcacattg tggcgcttat gagccccggt cccgatttct
     4861 tttttgtctc tcagaccgct gttagtcgtt cccgtaaaga agcgatgatg ggcgtgctgg
     4921 gcattacctg cggcgtaatg gtttgggctg ggattgcgtt gcttggccta catttgatta
     4981 tcgaaaaaat ggcctggctg catacgctga ttatggtggg cggtggcctg tatctctgct
     5041 ggatgggtta ccagatgcta cgtggtgcac tgaaaaaaga ggttgtttct gcccctgcgc
     5101 cacaagtcga actggcgaaa agcgggcgca gttttcttaa gggcttactg accaatctcg
     5161 cgaaccctaa agcgattatc tactttggtt cggtgttctc gctgtttgtc ggtgataatg
     5221 tcggcaccac cgaacgctgg ggcattttcg cgttgattat tgtcgaaaca ctggcgtggt
     5281 ttaccgtcgt tgccagcctg tttgctctgc cgcaaatgcg ccgtggttat caacgtctg
//
LOCUS       DAZFFE010000190         5321 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFFE010000190 DAZFFE010000000
VERSION     DAZFFE010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.191"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(162..863)
                     /locus_tag="ACHJ69_004658"
     CDS             complement(162..863)
                     /locus_tag="ACHJ69_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO5017467.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRKNGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNASELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTSL
                     YQNMRANALTDTELRRKAADELTCMTARINRGEAIPEPVKQLPVMGGRPLNRVQALAK
                     IAEIKAKFGLKGVMK"
     gene            complement(860..1879)
                     /locus_tag="ACHJ69_004659"
     CDS             complement(860..1879)
                     /locus_tag="ACHJ69_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO5017468.1"
                     /translation="MTGSEMAKAGLREQSRLSGANRNALIAEGIMANTAEIFNFPVPD
                     EAQKEPRVADLDDGYTRIANELLEAVMLAGLTQYQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNIGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKDVYPNQVNTKDKLTKDNIKPFSSENSGESSDQSENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWI
                     YGVDL"
     gene            complement(1876..2414)
                     /locus_tag="ACHJ69_004660"
                     /pseudo
     CDS             complement(1876..2414)
                     /locus_tag="ACHJ69_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
     gene            complement(2484..2714)
                     /locus_tag="ACHJ69_004661"
     CDS             complement(2484..2714)
                     /locus_tag="ACHJ69_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5017469.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPELFPHTEKNAA"
     gene            2819..3508
                     /locus_tag="ACHJ69_004662"
     CDS             2819..3508
                     /locus_tag="ACHJ69_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO5017470.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSIVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            4104..4310
                     /locus_tag="ACHJ69_004663"
     CDS             4104..4310
                     /locus_tag="ACHJ69_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO5017471.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            4386..4682
                     /locus_tag="ACHJ69_004664"
     CDS             4386..4682
                     /locus_tag="ACHJ69_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO5017472.1"
                     /translation="MNAYYIQDRLEALSWARHYQQIAREEKESELADDMEKGLPQHLF
                     ESLCIDHLQRCGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            4688..>5321
                     /gene="bet"
                     /locus_tag="ACHJ69_004665"
     CDS             4688..>5321
                     /gene="bet"
                     /locus_tag="ACHJ69_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO5017473.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNSIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYEKDEAERIVENTAYTAERQPERDITPINDETMQEINTLLIA"
BASE COUNT         1337 a         1319 c         1246 g         1419 t
ORIGIN      
        1 caaaaataaa ataaaatctc tgcataaact ttatggctaa atgatttcac aacaatggaa
       61 gagacacttt gttactcacg caatcaatca tttcgggcat aacaactagt cctagattat
      121 attcgctagg tatactttat ttttaagcta atactttagc tctatttcat cactcctttc
      181 agcccgaact tagctttgat ttctgcgatc ttcgccagcg cctgaacacg atttagaggt
      241 ctaccgccca tgacaggaag ttgttttact ggttcaggta tcgcctcacc acggttaatt
      301 cgcgcggtca tacaagtcag ttcatcggca gccttgcgcc gtaattccgt atcagtaagc
      361 gcattggccc gcatattctg gtacaggcta gtaaccagcc agtagtgcgc gtttgatttc
      421 cacggataag actctgcatc cggatacagg ccacgcttcc ggcaatactc gtaaaccata
      481 tcaaccagct cgctggcgtt tggcagtccg gcaataacgg atgcttcttc acggcaccat
      541 gcaacaaact gcccgggtga tggcaggaat ggtcgattct gccgacgggc tacgcgcatt
      601 cctgcgttaa cctgttccat tgtggtgatc ccgtttttcc ggaaagccag cacccactgg
      661 cggcgaattt cgttcagttc gttctggtcc cggttagcca ggctcgccgg gaaagttgcc
      721 agtaactggc tgaacacacc attgatgatc tgcgctacct gttgtacctg cggcttttcg
      781 tcgtactgtt ccggcatatt gttggcgatc cgacgcatct gctcacggtc aaagttaacc
      841 atctgtgcgg cgatgttttt catagatcca ccccgtaaat ccagtcagtg tttgtcaggt
      901 cgagttttgg tttgctagct gtcacgccag cctgttgctt gttacggttg atttcgagtt
      961 gggtccactt gtcgcggagt ttggccggac ttagcacgtt accggaccag aagttgtcct
     1021 ggcatgccca gcggaacagt acacacatgt cgcggtggtt acgtccgtca cgttcacgca
     1081 tcaggcggat atcgttagcc caccctgcaa aattcggttt tctggctgat ggcgcgatgg
     1141 tcttcaccat gtcaaacatc cactctgcgg cggtcaggtc ttctgctgtc ccccacttgc
     1201 tgccgctctg aattgcagca tccggtttca ccacaggaag atcgttttct gactggtcag
     1261 aggattcgcc agaattctcg gacgaaaaag gttttatatt gtcttttgtt agtttgtctt
     1321 ttgtgtttac ctgattcggg taaacgtctt tacctgattt gggtaaactt ttcttacctg
     1381 attcaggtaa atttacctct ttcaggtaaa ctttattttt cttacctgat tcgggtaatg
     1441 ttgaccattc actgaccaca ttattaatgc cgatattccg cccgctctga ataaaaatcc
     1501 cacgctttac cagaacactt tttgcagcag aacacttgtg cggcaatatc ccggtcaact
     1561 cggaaagttg ctcgttgctc acccaatcca gttttttatt aaagccatat gttttgcgca
     1621 tgacagccag gaagaccaga agctggtact gtgttaatcc ggccagcatc acagcttcca
     1681 gcaactcatt tgcaatgcgc gtataaccat catcgagatc tgccacgcgc ggctcctttt
     1741 gtgcctcatc cggcactgga aaattgaata tctcagcagt gtttgccata attccttccg
     1801 caatgagtgc gttacgattt gcacctgaaa gtcggctctg ttcccgcaga ccggctttcg
     1861 ccatttctga acctgtcata ttgcccccag catggtggta accatcgcca ttaatggacc
     1921 agccagatcc gggtccacac gaaacatcga cacaatgcct tcactcatct ccttcagttt
     1981 ctggtggcgt ggtgcgttga gaatgaccgc ctgctttgcc tcactgagtt ccttttccat
     2041 ttcagccagc cgagccatga agctatcctg ctcaaccagg tggccgcgat attccagcgg
     2101 tagtaccgcc agaattgccg gggtcagttc acgcacgtta tttcggtatt tttcagaatc
     2161 gaatttgtta tcgaggaagc ggaacagctt ctggcgtgca cggctgacat catcagggaa
     2221 atcgatggtg ccgccgccct gctcccgata ctcattcaca atgagtgcgg caacaacatc
     2281 ctgattatct gcagccgacc aggcgcgaac ggcatcacgg atttttcgtg gcctggcgcc
     2341 tgttttgttt gagaacgatt tatcaccgca gtcgggctaa atccgctagt ctgttggtat
     2401 gtaagtggtt gcataattga ctcctttagt ttgaattgac tgttaagttg attgcttatt
     2461 gttaaagagc gtgaaatgga aatttaagct gcgttctttt cggtgtgtgg aaacaactcc
     2521 ggaagatccg ggcgaatctg gtatgccttc actactccac cagtagccgt aacaatgctg
     2581 ccgacatgtt caggggatac ctttgctttg ttgtgaagcc acttatagac ggcctgctgt
     2641 gaaacttcgc aagcagcgcc cagtttcttt tgtgaaccaa cgatattgat cgctgttttg
     2701 atagctgggt tcataacaac ctccgtggtt aatttgaatc aagattaaaa ctatggttgt
     2761 ttttaatcaa caaccatttt cgtttgatgg aataaaacct tggttgtaca tttggactat
     2821 gaaaacaaca ctctcagaaa gacttaaaga agccagatta gcgcgaggcc ttacacaaaa
     2881 ggcgcttggg gatttggtcg gggttagcca ggctgctatt cagaaaatcg aaacagggaa
     2941 agctaatcaa acaactaaaa tcgtggagat cgcgaacgct ttgggtgtgc gcgcagaatg
     3001 gttatcttct ggcgttggaa atatgtcaga cagtatagtg caaccaatac aatcaactgt
     3061 cagccattcc aaatacttca agattgacgt tcttgatata gaagtcagtg ctgggccggg
     3121 agtcatcaac cgtgagtttg tagaagttct acgctcggtt gagtactcgt ttgacgatgc
     3181 tcgtcacatg ttcgatggta ggaaggcgga aaatatccgc atcattaacg tgcgtggtga
     3241 cagcatgtca ggaacgatcg aaccaggtga tctgctgttc gttgatatca cagttaaatc
     3301 tttcgacggt gatggtatct atgcgtttct gtacgacgac acagcccatg taaagcgcct
     3361 gcaaatgatg aaggataagc tgctggtcat ctctgataac aaaagctact caccgtggga
     3421 cccgatcgag aaagacgaga tgaaccgggt gttcatcttc ggtaaggtta ttgggagcat
     3481 gccgcagaca tataggaagc atgggtagta ccaattaaaa attatcaact gggcattgtg
     3541 ctcattcagt aaagaactaa ttcctatctt tgctctaggt agtaatatta agccaccgca
     3601 ataatatctt tacctaacgg cgtaagaatc ccggtcaccg tgccgggttt tcttttgccc
     3661 tcccctcatc acacacaccg ttaaaaaaac caccataacc tcgcttcagt tatcgctatg
     3721 cgattcaagt cacaaaataa atccatccta aatacaacca gttatatcta aaacaaccaa
     3781 taaaacaact tttgttgttg ataataaaac aactatagtt ttaaataggt tcatcgcaac
     3841 aacacaacga tacggcaact acctgattca ccgttgcgat gaccgcttag atccgcagtt
     3901 tgaatttcag caggcttcgg ggagtgcgag gggtgaaacg gacgcgtgaa cgtcggtgtg
     3961 accagctgaa atcaactcaa catttcatac cttagtcgct tcaacgaggc ggcttagtta
     4021 tgacaaccgg cggccatcca ccgcctgaat acgcgcagaa gtctctatat gttcagcagc
     4081 ccagcttacg ggcaggagtt tttatggttc atcaacatta cggaacgcag accgttaatc
     4141 gcggcgcggt catgccagga atgctggtca aacacaaaga tggtacctgg actgcatcag
     4201 ctaatttacg cggacggctt tatctgcatc gcggcatcga gcgcacttat acccgtgatt
     4261 tgctcgtgga agtttttctc gacggacgcg gtaacggcct gaatcactaa tcccctttcc
     4321 tgttttccta atcagcctgg catttcgcgg gcgatatttt cacagccatt ttcaggagtt
     4381 cagccatgaa cgcttattac attcaggatc gtcttgaggc tctgagctgg gcacgtcact
     4441 accagcagat tgcccgtgaa gagaaagagt cagaactggc agacgacatg gaaaaaggtc
     4501 ttccacagca cctgtttgaa tcactctgca tcgatcattt gcaacgctgc ggggccagca
     4561 aaaaagccat tacccgtgcg tttgatgacg atgttgagtt tcaggagcgc atggcagaac
     4621 acatccggta catggttgaa accattgctc accaccaggt tgatattgat tcagaggtat
     4681 aaaacggatg agtacagcac tcgcaacgct ggcagggaag ctggctgaac gtgtcggcat
     4741 ggattctgtc gacccacagg aactgatcac cactcttcgc cagacggcat ttaaaggtga
     4801 tgccagcgat gcgcagttca tcgcattgtt gatcgtcgcc aaccagtacg gccttaatcc
     4861 gtggacgaaa gaaatttacg ccttccctga taagcagaac agcatcgttc cggtggtggg
     4921 cgttgatggc tggtcccgca tcatcaatga aaaccagcag tttgatggca tggactttga
     4981 gcaggacaat gaatcctgta catgccggat ttaccgcaag gaccgcaatc atccgatctg
     5041 cgttaccgag tggatggatg aatgtcgccg cgaaccattc aaaacccgcg aaggcagaga
     5101 aatcacggga ccgtggcagt cgcatcccaa acggatgtta cggcataaag ccatgattca
     5161 gtgtgcccgt ctggccttcg gatttgctgg tatctatgaa aaggatgaag ccgagcgcat
     5221 tgtcgaaaat accgcataca ctgcagaacg tcagccggaa cgcgacatca ctccgattaa
     5281 cgatgaaacc atgcaggaga ttaacactct gctgatcgcc c
//
LOCUS       DAZFFE010000191         5260 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFFE010000191 DAZFFE010000000
VERSION     DAZFFE010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.192"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..318
                     /locus_tag="ACHJ69_004666"
     CDS             <1..318
                     /locus_tag="ACHJ69_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5017474.1"
                     /translation="FERVWREYPHRAGSNPKRAAFNSWKARLREGVSPDAVLDGVRRY
                     SRYLEATGKAGTEFVQQASTFFGPNRNFENPWSLPKAGAVSQRCVNHISEPDTEIPPG
                     FRG"
     gene            325..1065
                     /locus_tag="ACHJ69_004667"
     CDS             325..1065
                     /locus_tag="ACHJ69_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5017475.1"
                     /translation="MKNISTGGVLERVRRLAPPHVTAPFRTTDEWREWQLAEGRKRSE
                     EINRLNHQTRVEKILNRSGIQPLHRKCSFGNYRVQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGQSGEKFLQE
                     LCGVDLLVLDEIGMQRDTRNEQVILNQIVDRRTASLRTVGILTNLNHAAMSTLLGDRV
                     MDRMTMNGGRWVNFNWESWRSNVGRQGM"
     gene            1091..1861
                     /locus_tag="ACHJ69_004668"
     CDS             1091..1861
                     /locus_tag="ACHJ69_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO5017476.1"
                     /translation="METVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGESGVTEGQALQPEAPDAVTEEVAPKVTADMMIEFIAQKGATTCEEL
                     ADKFGVTTRKVASTLAVVTATGRLVRVSIKGRFHYKKPDVENGDLSASSSQDEQSHHE
                     AENSAAGDEMNRFPLASESTAKASTDEFIREVPSFTEKSASSVMLPPLHAASRELRRA
                     KKQVQKWERVCAALRELNKHREIVMQITDSSSSIASEK"
     gene            1876..2319
                     /locus_tag="ACHJ69_004669"
     CDS             1876..2319
                     /locus_tag="ACHJ69_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO5017477.1"
                     /translation="MAKVFTPEQREELKARIVELVRLNGRGTFRQLATETGVSKTAVS
                     RLSRELVVSGAIFDSGYGLFPSEQARKDWQNARKKLSRAKSKKPLVVDPGLIRALPDG
                     EIRRYDRRYNIICRECRKSEVMQRVLTFYRRCVQENNSLRSRTLT"
     gene            2333..2833
                     /locus_tag="ACHJ69_004670"
     CDS             2333..2833
                     /locus_tag="ACHJ69_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017478.1"
                     /translation="MHLNKEHQAMMGKRQTEEAVRDLEKLLGNRETKLLFLVPVIRQK
                     VEEILEENGIPKEYMTDAMEYSLELVRITPDLTWLDEYQQQFEKGAGTLLSVADKTEQ
                     KTEPSLFWNTVEAIARSKMFEFNNINHSGRDTHQSSHLEGVSMLLALLREQGECPQST
                     LNDTEQ"
     gene            3135..3392
                     /locus_tag="ACHJ69_004671"
     CDS             3135..3392
                     /locus_tag="ACHJ69_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017479.1"
                     /translation="MNNKINIPLEIKEINEIIEVTDRPEFTLMRRYETGTDEQKYIMV
                     AALAVMAIERERMEKDVMVIPARNDSPDLRWQEPKWDVAIR"
     gene            complement(3443..3748)
                     /locus_tag="ACHJ69_004672"
     CDS             complement(3443..3748)
                     /locus_tag="ACHJ69_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO5017480.1"
                     /translation="MTMFNPPHPGEIIADILEGQNIGSRELARALDVAPSTVQRLVSG
                     NATISPEMAVRLAAVLGGTPSSWIRLQTAWSLEKAEREVDVSHLSTKYRPAEISPHA"
     gene            complement(3759..4079)
                     /locus_tag="ACHJ69_004673"
     CDS             complement(3759..4079)
                     /locus_tag="ACHJ69_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5017481.1"
                     /translation="MSKNYRSDALASVHEMMESLHDIGAVTKQTMREFDELCLQPAPS
                     MSPERIRALREREHLSQPVFARYMNVSKNLISDWERGVKRPGGPALRLLSVIEKNGIQ
                     AINV"
     gene            complement(4072..4443)
                     /locus_tag="ACHJ69_004674"
     CDS             complement(4072..4443)
                     /locus_tag="ACHJ69_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5017482.1"
                     /translation="MRIFKNAWFERFARKNKISDQSLREIVKQADNGLISANLGNGVI
                     KQRLPRVGGGKSGGYRTIIFYRVGMRAFFVYAYAKNERENITATEESTFRKAAPHVLN
                     LTDEQLAQLILQGQFTEVPNE"
     gene            4785..>5260
                     /locus_tag="ACHJ69_004675"
     CDS             4785..>5260
                     /locus_tag="ACHJ69_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO5017483.1"
                     /translation="MTVQISVKNLSPVTYNQIPVITTELLACLYGTETIRIQQNHHEN
                     KSRFIEGKHFFKAVGDELRNLRLVLNESQNEVRISPKTRSLILWTERGAARHAKMLET
                     DQAWEVFEKLEDCYFSQGKTTQTEQQPQIQPQFTAEEIILLCYMLVWMEKAQDLSK"
BASE COUNT         1438 a         1192 c         1379 g         1251 t
ORIGIN      
        1 ttcgaacggg tctggcgtga atatcctcat cgggcagggt caaacccgaa gagagccgca
       61 ttcaattcct ggaaggccag attacgcgag ggggtgtcac cggatgccgt gctggatggc
      121 gtgaggcgtt actcaagata cctggaggct accgggaaag cgggaactga atttgttcag
      181 caggcatcga cgttttttgg tccgaacagg aatttcgaaa atccgtggtc gctgcctaag
      241 gctggcgcag tcagccagcg ttgcgtgaat cacatttctg aaccggatac tgaaattccg
      301 ccgggtttca gggggtaatc agccatgaaa aacatttcga ctggaggggt tcttgaacga
      361 gtgcgccgcc tggcaccacc gcacgtgaca gcaccgttcc ggacgaccga cgagtggcgg
      421 gaatggcaac tggctgaggg ccgtaagcgc agcgaggaaa ttaaccgcct gaatcaccag
      481 acgcgggttg aaaaaatcct gaaccgttcg ggtattcagc cgcttcaccg aaagtgttca
      541 ttcgggaact accgggtgca gaacgacgga cagcgccacg ctctgagcca ggcgaaatcc
      601 atcgctgacg aactgatgac cgggtgtaca aacttcgtgt tcagcggtaa acctggtacc
      661 ggtaaaaatc acctggcagc agcgattggc aaccggctga tggcgaaggg gagaagcgtg
      721 attatcgtca ccgtgtccga tgtcatgagc gtgttgcatg acagctacga caacggccag
      781 tccggggaaa aatttttaca ggagctttgt ggagttgacc ttctggtcct tgatgaaatt
      841 ggcatgcagc gggatacgcg caacgagcag gtcatactga accagatcgt tgaccgcaga
      901 acggcatcac tgcgcactgt cgggatcctg acgaacctga atcacgcagc gatgagcaca
      961 ctcctcggcg atcgggtgat ggaccgtatg accatgaatg gtggtcgttg ggtgaatttt
     1021 aactgggaga gctggcggtc aaacgttgga cgtcagggta tgtgagaatt tttgacgagg
     1081 taaattttcg atggaaaccg tattgcatgc actgaaagcg atgggaaaag ccaattctgt
     1141 tgaactggcg gcgcggcttg atatcagccg tgaagaagtt ctcaacgaac tgtgggaact
     1201 caaaaaaaat ggcgttgttg ataaaacggg tcacacctgg tttctggctg tcgaaggtga
     1261 atccggggta accgaagggc aggcactaca acctgaagcg ccggatgcgg taaccgaaga
     1321 ggtcgctcca aaagttaccg ctgacatgat gattgagttt atcgctcaga agggggctac
     1381 aacctgtgag gaactggcgg ataagttcgg agttaccact cgtaaggttg cttccacgtt
     1441 ggcggtggta acagcaacgg ggcgcctggt acgtgtctcc atcaagggaa gattccatta
     1501 caaaaagcct gacgtcgaaa acggcgattt gagcgcgtca tcatcgcagg atgaacaaag
     1561 ccaccatgaa gcagaaaaca gcgcggcagg cgatgaaatg aatcgttttc cgttggcatc
     1621 agagagcacg gcaaaggcca gtacagatga gtttatccgg gaagttccat cattcaccga
     1681 aaaatctgcc agttcggtga tgcttccacc gctgcatgct gccagtcgag aacttcgtcg
     1741 cgccaaaaaa caggtccaga agtgggagcg tgtttgtgcc gcactacgtg agctgaataa
     1801 acaccgggaa attgtcatgc agattaccga ttcatccagc agcattgcat cagaaaagtg
     1861 ataactggag agcttatggc aaaagtattt acacccgaac agcgggaaga actgaaggcg
     1921 cgtattgtgg agctcgtgcg cctgaatggt cggggaacgt tcaggcaact ggctactgaa
     1981 accggagtca gcaagacagc tgtcagccgt ttatccagag aactggttgt cagtggcgct
     2041 atattcgact caggttatgg tttattcccg tctgagcagg cgcgtaagga ctggcaaaac
     2101 gcccgcaaaa aactctcacg agcaaagtcg aagaaaccgc ttgtcgttga tcccggtctt
     2161 atccgggcat tacctgatgg ggaaatccgg cgctacgaca gacgctacaa cataatttgt
     2221 cgagagtgcc ggaagagcga agttatgcag cgtgtactga cattctatag acgttgcgta
     2281 caggaaaata acagtctgcg tagccgcaca ttaacttaac gaggagctaa acatgcattt
     2341 gaataaagag catcaagcta tgatgggcaa gcggcagaca gaagaggctg tgcgcgatct
     2401 ggaaaaacta ctcggtaatc gtgaaacaaa actgcttttc ctggttccgg ttattcgcca
     2461 gaaggttgag gaaattcttg aagaaaatgg catcccgaaa gaatacatga cggatgccat
     2521 ggaatattca ctcgaactgg tacgaattac tcctgatctg acctggcttg atgagtatca
     2581 acaacaattt gagaaaggcg caggtacgtt gctttctgtt gctgataaaa cagagcagaa
     2641 aacagaacca tcgctattct ggaataccgt tgaggctatt gccaggagca agatgtttga
     2701 gtttaacaat atcaatcatt ccgggagaga cactcatcaa tcatcacatc ttgagggggt
     2761 gtccatgctt ctagctctgt tgcgagagca aggcgaatgt cctcagtcga cattgaacga
     2821 cactgagcaa tagtccatcc gtattttccg gcaagataaa gatatattgc gtcaaagcca
     2881 taaatagtcc ttggataccc ctctttaata gccagagagt ccccgaagca ttcaagaaag
     2941 taatttgagc gggctgtttc gtaatgaaaa ttccagcgag cctgattgag tttttttcca
     3001 tttatcctcc attgttgctc tgttttgaga aatggagacc ttcacgctgc aacgtgaggt
     3061 cgtgcgccgg acacgggtaa acatccggta ttcaaaattt aatgttaacg aatttttaat
     3121 attgaggtta tgacgtgaac aacaaaatca atattcctct cgaaattaaa gaaattaacg
     3181 aaatcattga ggttactgat cgccccgagt ttaccctcat gcgtcgttat gaaacaggaa
     3241 ccgacgaaca aaaatacatc atggttgcag cattggctgt gatggcgata gaaagggagc
     3301 ggatggaaaa ggacgtaatg gttattccgg cgagaaatga ttcaccggat ttgagatggc
     3361 aggagccgaa atgggatgtg gcaatccggt gattgatatt tctctggggg agggaaacgc
     3421 cgccagaacg acggcgcggt ggttaagcat gaggcgaaat ttctgccggg cggtattttg
     3481 ttgagagatg agatacgtca acttctcttt ccgctttttc aaggctccat gccgtctgga
     3541 gacgaatcca tgaagacgga gttccaccca ggacagcagc gagacgaaca gccatttcag
     3601 gagatattgt cgcattacct gaaaccagtc gctgaacagt ggaaggcgcg acatcaagcg
     3661 ctcttgccag ttctctgctc ccaatattct gaccttccag aatatcagca ataatttctc
     3721 cgggatgagg gggattaaac atcgtcattt agtggtaatc aaacatttat ggcctgaatc
     3781 ccgttttttt caataacgga aagaagtcgc agggcgggac caccagggcg tttcactccg
     3841 cgttcccagt cagatatcag gtttttactg acattcatat atctggcaaa gacgggttgt
     3901 gacagatgtt cacgctcacg gagtgcccgg attctttcag gagacattga aggtgctggt
     3961 tgcaggcaga gttcatcgaa ttctcgcata gtctgtttcg ttactgcgcc gatatcatga
     4021 agcgattcca tcatttcatg tacggatgca agcgcatcac tgcggtaatt tttactcatt
     4081 tggtacctcc gtaaactgac cctgtagaat cagttgtgcc agttgttcat ctgttagatt
     4141 gagtacgtga ggtgcagcct tccgaaatgt gctttcctca gtagcggtta tattttcacg
     4201 ttcatttttt gcatatgcat atacgaaaaa tgccctcata ccgacacgat agaaaatgat
     4261 tgtgcgataa ccgccagatt tgccgccacc gactcgtggc agacgttgct tgataacgcc
     4321 gttacccagg tttgctgaga taagcccgtt atcagcctgt ttaacaattt cgcgcagtga
     4381 ctggtcggat attttgtttt tgcgtgcaaa tcgctcaaac caagcgtttt taaaaatgcg
     4441 cattttttgt tccgttatta atgtataaca catagtatta cattgtgtag ggatctgcaa
     4501 tgccatcagt ttgacaacgt cgcgtatccg ggattatatt catcgcacgc cagcaaaatc
     4561 tggcgtcggg attggaaccc cggatatcga aacggtgcat aaccgcgctg gcggtttttt
     4621 tatgcgctaa gcacagtcac attcgcgatt tatggtgggc tgtgtggggg caccgaaagg
     4681 tgcgccggat gtttcgaccg gtagttccaa ccctgcacag tccgccaccc gatgattgga
     4741 acctgacggt ggtgaggtaa aaattatcga aacgcgaggt cgttatgact gttcaaatct
     4801 ctgtcaaaaa cctttcccct gttacctata accagattcc tgtaatcaca actgagttac
     4861 tcgcctgcct ttatggaaca gaaacaatcc gtatacagca gaatcaccat gaaaataaat
     4921 ctcggtttat tgagggaaag cacttcttta aagctgttgg tgatgaactc agaaatttgc
     4981 gactagtttt aaacgagtcg caaaatgagg tgagaatctc tcctaaaact cgctccctca
     5041 tcctctggac agaacgcggt gctgcccgtc atgcaaaaat gctcgaaacc gatcaggcgt
     5101 gggaagtgtt cgaaaaactg gaagactgct atttcagcca gggaaaaaca acacaaaccg
     5161 aacagcaacc gcagattcag ccacaattca cagccgaaga aatcatcctc ctctgctaca
     5221 tgctggtatg gatggaaaaa gcccaggacc tcagcaaaca
//
LOCUS       DAZFFE010000192         5255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZFFE010000192 DAZFFE010000000
VERSION     DAZFFE010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.193"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..129)
                     /locus_tag="ACHJ69_004676"
                     /pseudo
     CDS             complement(1..129)
                     /locus_tag="ACHJ69_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            431..958
                     /locus_tag="ACHJ69_004677"
     CDS             431..958
                     /locus_tag="ACHJ69_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5017484.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMKEQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQVQPQPQQNGQSQSADATKKGSAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            1016..1249
                     /locus_tag="ACHJ69_004678"
     CDS             1016..1249
                     /locus_tag="ACHJ69_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO5017485.1"
                     /translation="MSEYFRILQGLPDGPFTRKQAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            1310..3274
                     /locus_tag="ACHJ69_004679"
     CDS             1310..3274
                     /locus_tag="ACHJ69_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO5017486.1"
                     /translation="MSVTKSEPKSTRKASRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKIISQELATAASMTENGHRRDMHPAEQIAGFCAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDLITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWGWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAADDADRTEQVQEKAPVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKDGAAFLALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSIHGVQTREHGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLQKPQIIDALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCTPRPQAETETTEYRDDQAEAA"
     gene            3343..3777
                     /gene="psiB"
                     /locus_tag="ACHJ69_004680"
     CDS             3343..3777
                     /gene="psiB"
                     /locus_tag="ACHJ69_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO5017487.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            3774..4536
                     /locus_tag="ACHJ69_004681"
                     /pseudo
     CDS             3774..4536
                     /locus_tag="ACHJ69_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            complement(4514..4693)
                     /locus_tag="ACHJ69_004682"
     CDS             complement(4514..4693)
                     /locus_tag="ACHJ69_004682"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017488.1"
                     /translation="MKINLLRGYLLSATQHGNKPPSRHEAESLKRRAHHSPYTCTLTT
                     LTFPENNPLIQAVYG"
     gene            4715..4864
                     /gene="mok"
                     /locus_tag="ACHJ69_004683"
                     /pseudo
     CDS             4715..4864
                     /gene="mok"
                     /locus_tag="ACHJ69_004683"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            4806..4931
                     /locus_tag="ACHJ69_004684"
     CDS             4806..4931
                     /locus_tag="ACHJ69_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFO5017489.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK"
BASE COUNT         1180 a         1398 c         1629 g         1048 t
ORIGIN      
        1 tgtcgcgccg tggcctgcgc tcccggcatc ccggcaaaag acgccgggat gtaaggagca
       61 acgccgtttc gcaaaccgcg cttcacgtcg ttgcggcggg tccctgcccg ttgttctgtc
      121 cggtgccatt cacctgttta agcccctgtc gccgggggca tagacagcca caacctgccg
      181 cactgactgt ccagggtaaa tgaacagccc acaaaatttt ttacccaaaa cccggtaaaa
      241 aattttgcgt tctgcccagg acaggtgcgt caggccgtgg cagtgatgcc ccatgcggcg
      301 aaggttctca aacaggtgat ggtgaatacc ggagggaaca aaaggcaggt ccccgcaaaa
      361 cgtcaaaacc gcggctcccg aaactcaaca gatgatgggg gctgaaaaca tcaacagaag
      421 gagacacatc atggcagttc gtggcattaa caaggtcatt cttgtcggtc gtctgggaaa
      481 agatccggaa gtccgttaca tccccaacgg gggcgcagtg gcaaacctgc aggtggccac
      541 gtcagaaacc tggcgcgaca aacagacggg ggaaatgaag gagcagacgg aatggcaccg
      601 cgtggtgctg ttcggcaagc tcgcggaagt ggcaggtgaa tatctgcgta agggggcgca
      661 ggtctacatt gaaggccagc ttcgcacccg tagctgggaa gataacggta ttacccgtta
      721 cgtcaccgaa atccttgtta aaaccacggg caccatgcag atgctgggga gtgcaccaca
      781 gcagaacgct caggtgcaac cgcagcctca gcagaatggt cagtcacaga gtgctgacgc
      841 gacgaaaaaa gggagcgcga aaacgaaagg ccgtggacgt aaggctgcgc agccggagcc
      901 tcagccgcaa ccaccggagg gggaggatta cgggttttca gacgatatcc cgttctgact
      961 ggcctgactg tgataaccgc cccgtcctgc gcggggcatc accggagata tgaggatgag
     1021 cgaatatttc agaatacttc agggactgcc tgacggtcct tttacccgca aacaggccga
     1081 agccgttgcc gcacagtacc ggaacgtctt tatcgaggat gaccacggcg aacagtttcg
     1141 cctggttgtc cgtaataacg gaacaatggt ctggcgtacc tggaattttg aggacggtgc
     1201 cgggtactgg atgaaccatg tcatccgtga tttcgggatc cttaagtaag agaggtgccg
     1261 gacggggatc acgtccggca gacattaact acacaataag gggatagtca tgtcagtaac
     1321 aaagtctgaa ccaaaatcta cccgtaaagc aagccgtaaa cctgcaaaaa cgcaggaaac
     1381 tgtcctgtct gccctgctgg cgcagacgga ggaagtgagc gtgccgctgg cctcgctgat
     1441 taagtcacca ctgaatgtgc gcacggtgcc gtattctgcg gagtccgtca gcgaactggc
     1501 tgattccatt aagggggtcg gcctgctgca gaatctggtt gttcatgccc tgccaggtga
     1561 ccgccacggt gtcgccgcag gtggtcgccg actggcagca ctcaacatgc tggcagagcg
     1621 tggcatcatt ccggctgact ggcctgtccg cgtgaaaatt atttcgcagg aactggcgac
     1681 ggccgcatcg atgaccgaga acggtcatcg tcgggatatg cacccagccg aacagattgc
     1741 cggattctgt gcaatggcgc aggaaggcaa aacacctgca caaatcggtg atttgctggg
     1801 ctattcaccc cgccacgttc agcgaatgct gaaactggct gaccttgcgc ctgtcatcct
     1861 cgatgcgctg gcagaagacc tcatcaccac cgagcactgt caggcgctgg cgctggagaa
     1921 cgacaccgcg cgtcaggtgc aggtgtttga agccgcctgt cagtcgggat ggggcggtaa
     1981 accggaagta cagaccattc gtcgtctggt gaccgaaagt gaagtggcgg tggcagggaa
     2041 cagtaaattc cgcttcgtgg gggctgatgc cttctcgcca gatgaactgc gcactgacct
     2101 gttcagcgac gacgagggtg gctatgtgga ctgcgtggcg ctcgatgccg ccctgctgga
     2161 aaaactccag gctgttgctg aacaccttcg ggaagccgaa ggctggggat ggtgcgccgg
     2221 acgcatggag cctgtcggtg agtgccgtga ggatgccgga acataccgct gtctgccgga
     2281 gccggaagcg gtgctgacgg aggcggaaga agaacgtctg aacgaactga tgacgcgtta
     2341 cgacgcgctg gaaaaccagt gtgaggaatc tgacctgctg gaagcagaaa tgaagctgat
     2401 gcgctgcatg gcgaaggtca gggcgtggac gccggagatg cgtgccggaa gcggtgtggt
     2461 ggtgtcctgg cgttatggca acgtgtgtgt ccagcgtggt gtgcagttgc gcagcgagga
     2521 tgatgcggct gacgacgctg accgcacgga acaggtgcag gagaaagcgc ccgtggagga
     2581 aatcagtctg ccactgctga cgaaaatgtc ttccgaacgc acgctggcag tccaggcagc
     2641 actgatgcag caaccggaca aatctctggc attgctggca tggacgctct gcctgaatgt
     2701 gtttggcagc ggagcgtaca gtaaaccagc acaaatcagc ctggaatgta aacattattc
     2761 gctgaccagc gatgcgccat cggggaagga cggtgccgca ttcctggcgc tgatggcaga
     2821 aaaagcccgt cttgcagccc tgttaccgga gggatggtca cgggatatga cgacgttcct
     2881 gtcactcagc caggaggtac tgttatccct gctcagtttc tgcaccgcgt gcagcatcca
     2941 cggcgttcag acccgcgagc atggtcacac gtcacgcagt ccgctggact cgctggagag
     3001 cgctatcgga ttccacatgc gcgactggtg gcagccgacg aaagcaaact tcttcggaca
     3061 cctgcaaaag ccgcagatta tcgatgccct gaatgatgcc ggactgtccg gtgctgcgcg
     3121 ggacgcggag aaaatgaaga aaggcgatgc cgctgaacat gcagagttcc acatgaaaga
     3181 caaccgctgg gtgcctggct ggatgtgtac accacgtcca caggcagaga cggaaaccac
     3241 ggagtaccgt gacgaccagg cagaagccgc ctgaccgcca gtcataaacc atttatcacg
     3301 ccgccctgcc ggagacgggg cggcagcaag ggagataccg tgatgaaaac tgaactgacc
     3361 ctgaatgtat tacagaccat gagcgcacag gaatatgaag atatccgggc tgccggaagt
     3421 gatgaacgtc gtgaactcac acatgccgtg atgcgggagc tggatgcgcc ggataactgg
     3481 acgatgaacg gcgagtatgg cagcgagttc ggcggctttt tccccgtcca ggtccgtttc
     3541 acgcctgccc acgaacgttt tcacctggcg ttatgttcac cgggcgatgt ctctcaggtc
     3601 tgggtgctgg ttctggtgaa tgccggtggt gagccgtttg cggtggttca ggtacagcgg
     3661 cggtttgcac cggaagccgt cagccattca ctggcactgg cggcgtcact ggacgcgcag
     3721 gggtacagcg tcagtgacat catccatatc ctgatggcgg aaggaggtca ggcatgagcg
     3781 cacgttcaca ggcactggtt cccctcagcg cagagcaaca ggccgcatgg cgggcggtgg
     3841 cggagacaga aaaacgccgc caccagggaa acacgctggc ggaatatccg tatgcggggg
     3901 cgtttttccg ctgtctgaac ggaagccgca ggatatcgct gtctgacctg cgatttttca
     3961 tgccgtccct gaccgcagaa gaactgcgcg gcaaccgttc tcagtggctg tatgccgtcg
     4021 atgtgctgat tgaaactcag ggcgaggtct gccttcttcc ccttcccggc gatgcagcag
     4081 agcggctgtt cccgtcggtg cggttccgtg tcagggagcg cagtcgccac aaatccgcac
     4141 tggtcatgca aaagtacagc cgccagcagg cgagggaggc agagcagaaa gcccgtgcgt
     4201 accaggcgct ggtcgctcag gctgaaatcg aactggcatt ccattcgccg gaaacagtcg
     4261 gaagctggca tgcccgctgg agtgaccggg tggcagagca tgacctggaa accctgttct
     4321 ggcagtgggg tgagcgtttt ccgtcactgg ccggaatgga acgctggcag tggcaggata
     4381 tgccgttctg gcaggttatt gcggaagcca gtcttgcagc aagagaggcc ggtcacgctg
     4441 tccgtgagat ggagcggtgg atggtgccga acaaactccg ggaggcggcg tgatgcggca
     4501 acaatcacac agattacccg taaacagcct gaatgagcgg gttattttca ggaaaagtga
     4561 gtgtggtcag cgtgcaggta tatgggctat gatgtgcccg gcgcttgagg ctttctgcct
     4621 catgacgtga aggtggtttg ttaccgtgtt gtgtggcaga aagaagatag ccccgtagta
     4681 agttaatttt cattaaccac cacgaggcat ccctatgtct agtccacatc aggatagcct
     4741 cttaccgcgc tttgcgcaag gagaagaagg ccatgaaact accacgaagt tcccttgtct
     4801 ggtgtgtgtt gatcgtgtgt ctcacactgt tgatattcac ttatctgaca cgaaaatcgc
     4861 tgtgcgagat tcgttacaga gacggacaca gggaggtggc ggctttcatg gcttacgaat
     4921 ccggtaagta gcaacctgga ggcgggcgca ggcccgcctt ttcagggctg atgctggtct
     4981 gactgcactg aagcgccttt ataaaggggc tgctggttcg ccggtggccc ctttctcctt
     5041 gctggcattg tacgggcatg aacaaccaga ctgccaggaa ggccgtttcc ctccgggaac
     5101 ggggatgaac gtgcgcctgc ggcgcccgtt cttttccccc gccttctctg tttatgactt
     5161 accagaaatc acatcacctt ccctttgatt attctctttt tcctcctgct ctgactctga
     5221 ttactgctgc cctgattatt gtctgttttg ttgtt
//
LOCUS       DAZFFE010000193         5090 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFFE010000193 DAZFFE010000000
VERSION     DAZFFE010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.194"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..348)
                     /locus_tag="ACHJ69_004685"
                     /pseudo
     CDS             complement(1..348)
                     /locus_tag="ACHJ69_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908615.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            419..964
                     /locus_tag="ACHJ69_004686"
     CDS             419..964
                     /locus_tag="ACHJ69_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO5017490.1"
                     /translation="MLIGYIRVSTNDQNTALQRNALECAGCELFFEDRISGKTRERPG
                     LKRALKRLRPGDTLLVWKLDRLGRSMQHLVLLTEELRQRGVNFRSITDNIDTSTPMGR
                     FFFHIMGALAEMERELIIERTRAGLAAARAQGRHGGRPQKLTEKEREQIARLLANGES
                     RKQIALIFDIGLSTLYRYFPA"
     gene            1078..3123
                     /locus_tag="ACHJ69_004687"
     CDS             1078..3123
                     /locus_tag="ACHJ69_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5017491.1"
                     /translation="MSDSKWKMLSAMPGEFSVKVAGGTVAFIESPFRPSGNKGGITFA
                     DCVIRFSTKEPLWVMPVSGNPSAEITSSGVTGIIPITSDVAGTLTPSDWNAPDNAGPS
                     GSTNGSSSEPEYYFVVILAGQSNGMAYGEGLPLPDSFDRPDPRIKQLARRSTVTPNGT
                     SCTYNDIIPADHCLHDVQDMSGINHPKADLAKGQYGTVGQGLHIAKKLLPYIPQNAGI
                     LLVPCCRGGSAFTTGADGSFSEASGASADSSRWGAGKPLYQDLVSRTKVALAKNPKNK
                     LLAVVWMQGEADLASGSQQHNSLFTAMVQQFRTDLAPLAAQCVSGNATTVPWICGDTT
                     YYWKNAGTDKYEAVYGGYKGKEAQKIFFVPFLTDENGQSTPTNAPAEDPDIVAVGYYG
                     AASRTQGSFVSTQRDSHFSSWARRGVISDRLASAIMLHAGRTAELMRGQTVTPPDEKP
                     SPDTPSKPSTPPADTTTMSTLFAYRASESEGRLTPQGWAAGGGKAQIVDDAGASGGKA
                     MKLTKETGRAPWYLEHDAGNGADLLGKGGLVSCRFKLDGALTANQYALALYWPVSALP
                     QGVTLAGHNLLASFYVQSDATDLNVMYHKGNTAQNTKLGSFGAFNNEWHTLGFRFAGN
                     NSIEVTPVIDGKDGTPFMLSQSPVGTFTADKLRVTDITKNATYPVLIDSIVVEVNNA"
     gene            3268..3450
                     /locus_tag="ACHJ69_004688"
     CDS             3268..3450
                     /locus_tag="ACHJ69_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO5017492.1"
                     /translation="MTFLQLILLYFCTAVCALYLVSGGYKAIRNYVRRKIEDAAAEKL
                     SKTAQATPLPKDPTQP"
     gene            3486..3740
                     /locus_tag="ACHJ69_004689"
     CDS             3486..3740
                     /locus_tag="ACHJ69_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO5017493.1"
                     /translation="MPEIKGTVTEDLVKQALYSGEVNKLLKAQVRKDFEAQIDTYVDE
                     VLAKLIGPAAAASDADNEPKTDAQPEQPEPAQPGTDSTMM"
     gene            3816..4016
                     /locus_tag="ACHJ69_004690"
     CDS             3816..4016
                     /locus_tag="ACHJ69_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017494.1"
                     /translation="MDKNKKQPDTKGLERLSAEIINALRSGLDEDTLQDRNFATAMWI
                     AMQILCTPRCAIFAVREGQLTR"
     gene            4201..4389
                     /locus_tag="ACHJ69_004691"
     CDS             4201..4389
                     /locus_tag="ACHJ69_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5017495.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSQSATEHP
                     TYTRKSCRGEQETLLSVR"
     gene            4355..>5090
                     /locus_tag="ACHJ69_004692"
     CDS             4355..>5090
                     /locus_tag="ACHJ69_004692"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFO5017496.1"
                     /translation="MGNKKHYFQSDKSLTYGSVCSGIEAATVAWSNLGWKARWFSEIE
                     DFPSTLLSYRWPHIPNLGDMALLAPRIRDGLIEAPDILVGGTPCQAFSMSGKRKGLSD
                     PRGQLTLSFVELANQIDTVRASLNKSPSIIVWENVTGVLSSHDNAFGHFLGALAGAGC
                     ALQPARKRWANAGVVSGPSRIVAWRVLDAQYFGVAQARRRVFVVASAGKRLDPGKVLF
                     EFPPVSQGDKNYYGKKKKSESLLDQGI"
BASE COUNT         1348 a         1245 c         1313 g         1184 t
ORIGIN      
        1 ccatgtacct ccgactaatt tttgcagggg tctgaatgct ataagatcgt ttgaagcatc
       61 aactttactg tcaatctgca ggcctgccat aacattaccg tacgcagagt aatatgcccc
      121 accgtacatt acctgcctgt tctctgctcc cagcctgata tcctggatat atcgggaatc
      181 cgcatcctgc tgtgttatac cagaagtgat gtttatgtcc tgcgtcccgt caaagggaac
      241 cccattaata tttcttgggg tggcgagtct attcgcggca acggcggtgc cgtccgatgg
      301 cagagcattc gccgccctgt ttaccgtttc ccgtaaaccg aggttttcag gaaagccagg
      361 attaactctg ttttattcct catgcgacaa ttccggcttt ttataataag gaaatcgtat
      421 gctgattggg tatatcaggg tgtcaacaaa tgaccagaat acggcgctac aaagaaatgc
      481 gctggaatgt gcaggatgtg agctgttttt cgaagacaga ataagcggta agacaaggga
      541 gcgtcccgga cttaaacgcg cattaaagcg actccggcca ggggataccc tgctggtctg
      601 gaaactggat cgccttggac gcagtatgca gcaccttgtc cttttaacag aagaactccg
      661 gcaacgcggc gttaacttcc gcagcattac ggacaatatt gatacatcca ctcccatggg
      721 acgtttcttt ttccatataa tgggcgcgct ggcggaaatg gagcgggaac tcattattga
      781 acgtacacgc gcgggtcttg ctgctgcccg tgctcagggg cgtcatggcg gcagacctca
      841 gaaactgaca gagaaagagc gggagcaaat cgcccgtttg ctggctaatg gcgagtcccg
      901 taaacaaatc gcactgatat ttgatattgg cctttccacc ctttatcgtt attttcctgc
      961 ctaaccggct ccgtcacaaa aagtaccgtg atgctttttt tgaatttccg caaaacacat
     1021 atttatcagc ggttgttatt ctgactctgt ttaattcaga aacaggcaga aaagacgatg
     1081 tcagactcca agtggaaaat gctctccgcc atgcccggag agttttcagt caaggttgcc
     1141 ggtggcacgg tggcatttat tgaaagccct ttccgccctt cgggtaataa aggggggatc
     1201 accttcgcag actgcgtgat ccgcttcagc acgaaagagc cattatgggt aatgccggta
     1261 tccggtaatc ccagtgcaga aatcaccagt tcaggcgtga ctggcatcat tccaatcaca
     1321 tctgacgtgg ccggaacgct cacgccctcc gactggaatg caccggataa cgccgggcct
     1381 tccggtagca cgaatggcag cagttcagag ccggaatatt actttgttgt cattctcgcg
     1441 ggtcagtcaa atggcatggc ctacggcgag gggctgccgt taccggattc gtttgaccgc
     1501 ccggacccac gcattaagca actggcacgc cgcagtacgg taacgcccaa cggtacgtcg
     1561 tgtacataca acgacatcat tccggcagac cactgcctgc atgatgttca ggatatgagc
     1621 gggataaacc acccgaaagc tgatctggca aaaggccagt acgggactgt cggtcagggg
     1681 ttgcatattg ccaaaaaact gcttccctat atcccacaga atgccgggat acttctggtg
     1741 ccctgttgtc gtggcgggtc agcattcacc actggagccg acggctcatt cagtgaggcc
     1801 agcggtgctt ccgctgattc ttcacgctgg ggggccggta aacctctgta tcaggacctg
     1861 gtaagccgca ccaaagtcgc gctggcgaaa aacccgaaaa acaaacttct ggctgtggtc
     1921 tggatgcagg gagaggcaga ccttgcctca ggaagccagc agcataatag tttattcacg
     1981 gccatggttc agcagttcag aactgacctg gctccgcttg ctgcgcagtg cgtgagtgga
     2041 aatgccacca cggtgccgtg gatttgcggt gataccacgt attactggaa aaacgctggc
     2101 accgataaat atgaggcagt atacggtggc tacaaaggca aggaagcaca gaaaattttc
     2161 tttgtaccgt tcctgacgga tgagaatgga cagagcacgc caacgaatgc tccggcggaa
     2221 gacccggata ttgtggctgt cgggtattac ggtgcagcat cccgaaccca gggcagtttt
     2281 gtttcgacac agcgtgacag ccatttcagc tcatgggcac gcaggggcgt catttcagac
     2341 cgtctggcct cagctattat gctccatgca gggcgcacgg ctgaactgat gcgcgggcag
     2401 accgtgacac caccggatga gaagccgtca cctgatacac catcaaaacc gtccacgcca
     2461 cccgcagaca ccacgacgat gagtacgcta tttgcttacc gggcatctga gtctgaagga
     2521 cggttgacac cacagggttg ggctgccgga ggtggcaagg ctcaaatcgt ggatgatgcc
     2581 ggagccagcg ggggtaaggc aatgaaactg accaaggaaa caggaagagc cccctggtac
     2641 cttgagcatg atgctggtaa tggtgcggac ctgctgggta aaggtggtct tgtcagttgt
     2701 cgttttaaac tcgatggcgc gcttacggct aatcagtacg cactggcgct gtactggccg
     2761 gtttctgccc tgccgcaagg ggttactctg gccggtcata acctgctggc atcgttctac
     2821 gtacagagcg atgccacaga ccttaacgtg atgtatcaca aaggaaacac ggcgcagaac
     2881 acgaagctgg ggtcattcgg cgcatttaat aacgaatggc atacgctggg cttccgtttt
     2941 gccggtaaca acagtattga ggtgacgccg gtcattgatg gtaaggacgg gacaccattc
     3001 atgctgtcgc agtctccggt cggcacgttt acggcagaca agttgcgcgt gaccgatatc
     3061 actaaaaatg cgacttaccc ggtactgatt gacagtatcg tggtggaagt gaataacgcg
     3121 taagcaggat aaaaaaatgc cgccggtaag ggaaaacaag aaaccggcgg caaaaagatc
     3181 gttatatcca aagcaaaaaa tgcagctcac ctttttacct aacagatatt accggtgtca
     3241 acaccatgtc aataattggg agggataatg acatttttac agcttatttt attgtatttc
     3301 tgcacggcag tatgtgcgtt atatctggtg agtggtggtt ataaggccat cagaaattat
     3361 gttcgccgca aaattgagga cgcagcagct gaaaaactca gcaaaacagc gcaggcaact
     3421 ccgctcccaa aagacccgac ccagccctga ttgcagggct ttattcccgc ataaggagga
     3481 tatacatgcc agaaattaaa ggcaccgtca ctgaagacct ggtaaaacag gcactgtatt
     3541 ccggtgaagt aaataagttg ctgaaagcgc aagtccgcaa ggattttgag gcacaaatcg
     3601 acacttacgt tgatgaggtg ctggcaaaac ttattggacc agctgctgcg gccagtgatg
     3661 ctgataatga accgaaaacc gacgcgcagc cagagcaacc agaaccggct cagcccggaa
     3721 ctgacagcac catgatgtaa caatacaaac cggcagtttc tcctgtcggt tttattttca
     3781 tgggcgcttt atataccaat cagaggacaa taatcatgga caagaataaa aagcaacctg
     3841 ataccaaagg cctggaaaga ttaagcgcag aaattatcaa tgcgctacgt tcaggacttg
     3901 atgaagatac attacaggat cgaaacttcg ccaccgcaat gtggatagct atgcagatcc
     3961 tgtgtacccc aagatgcgct atttttgcgg ttcgcgaagg tcaactgact cgctgattgc
     4021 acgaattaca tcagaaaagc acgcgttcca taactgcata gcggatgaat cacctccctg
     4081 aatcattgtt gatgactttt caatctattt gttgtatcaa ttaaacaaat ctaatcgatc
     4141 gtttttgtcg atcgattagg cgagaaagat atcatcaaca aagcaggagg ttatgcggcc
     4201 atgtatcgaa acatacggtg ccgacactgc aataagctac tggcacgggc cagtttcagt
     4261 tatctggaag taaaatgtcc gcgctgtaaa accctcaatc aaatcacatc tcagagcgcc
     4321 acagagcacc ccacatacac aaggaaatcc tgccgtgggg aacaagaaac attactttca
     4381 gtcagataag tcactaacct atggttcagt atgcagtggt attgaagctg cgacagtagc
     4441 ctggagcaat cttggctgga aagccagatg gttcagtgaa atagaggact tcccgtcaac
     4501 acttctttca tatcgctggc cccatatacc aaatcttggt gatatggctt tgcttgcgcc
     4561 tcgtatcagg gatgggctta ttgaagcacc agatattctt gtaggtggaa ctccgtgtca
     4621 ggcattcagt atgtcaggca aacgaaaagg cctttcagat ccacgggggc agttaacact
     4681 gtcttttgtt gaactggcga atcagataga taccgttcgg gcatcgctca ataaatcccc
     4741 ctcaataatc gtatgggaaa acgtcacagg agttttaagt agccatgaca atgcattcgg
     4801 tcattttctt ggtgcgctgg ccggagcagg ttgcgcatta cagccagcaa ggaaaagatg
     4861 ggcaaacgct ggtgttgtgt ctggaccatc gcgtatcgtc gcctggcgag tacttgatgc
     4921 ccaatatttc ggagtcgccc aggctcgcag aagagtgttt gttgtggcaa gtgctggaaa
     4981 aaggctcgat cccggaaagg tattatttga attcccgcct gtgtcgcagg gcgataaaaa
     5041 ctattacgga aagaagaaaa aatccgaatc ccttcttgat caaggcattg
//
LOCUS       DAZFFE010000194         4834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZFFE010000194 DAZFFE010000000
VERSION     DAZFFE010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.195"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..629
                     /gene="hprR"
                     /locus_tag="ACHJ69_004693"
     CDS             <1..629
                     /gene="hprR"
                     /locus_tag="ACHJ69_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310734.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO5017497.1"
                     /translation="VTQGLSEAGYVIDAVSDGRDGLYLALKDDYVLIILDIMLPGMDG
                     WQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPFSFSELLARVRAQLRQ
                     HHALNSSLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLASRAGEIIPRTVIASE
                     IWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLISTIRGMGYSFVAVKK"
     gene            629..1987
                     /gene="hprS"
                     /locus_tag="ACHJ69_004694"
     CDS             629..1987
                     /gene="hprS"
                     /locus_tag="ACHJ69_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO5017498.1"
                     /translation="MKRLSITVRLTLLFILLLFVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSNGMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNNEVENLLDYLEYLSDEKEICF
                     KVECNQHIFADKILLQRMLSNLIVNAIRYSPEKSRIQITSFLDTNGSLNIDIASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2094..2945)
                     /gene="hchA"
                     /locus_tag="ACHJ69_004695"
     CDS             complement(2094..2945)
                     /gene="hchA"
                     /locus_tag="ACHJ69_004695"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO5017499.1"
                     /translation="MTVQKSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESEDVAAALQWAIENDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(3539..4654)
                     /locus_tag="ACHJ69_004696"
     CDS             complement(3539..4654)
                     /locus_tag="ACHJ69_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO5017500.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGEHIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGENQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNYD
                     FFGLVDGLDFAIQYQGKNSNRSTKKQNGDGYALSVDYNINGFGIVGAYSKSDRTNDQV
                     ADGNGSNAELWSLAAKYDANNVYAAVMYGETRNMTPGSIDTGVADREGNTIMRDQLIN
                     ETQNFEAVVQYQFDFGLRPSLGYVYSKGKDIKGVPGHRYVDADRVNYIEVGTWYYFNK
                     NMNVYTAYKFNMLDKDDAAITGAAADDQFAVGIVYQF"
BASE COUNT         1362 a          974 c         1100 g         1398 t
ORIGIN      
        1 gggtaacgca ggggctttcc gaagcgggtt atgtcattga tgccgtttca gatggcagag
       61 atgggcttta tcttgcgctg aaggatgatt atgtattgat cattctggat attatgcttc
      121 cgggtatgga tggctggcag atcttacaaa cgttaagaac agcaaagcaa acccctgtta
      181 tttgccttac tgcaagggat tctgtcgatg acagagtcag agggctggac agtggggcaa
      241 atgattatct ggtaaaacct ttttcatttt ctgagttgct ggcaagggtt cgggcacaat
      301 taaggcaaca tcacgctctg aattcatcat tagaaatcag cggcttaaga atggactctg
      361 tcagccaaag tgtgagcagg gacaatatca gtattacact gacgcgcaag gagtttcagt
      421 tactttggtt actggcctcc agagctggcg agattatccc cagaacggtt attgcgagtg
      481 aaatttgggg aatcaacttt gatagtgata ccaatacggt ggacgtcgcc attcgcaggc
      541 tccgcgcaaa agttgatgat ccttttcctg aaaagctaat ctccacaatc cgggggatgg
      601 gctattcatt cgtagcggta aaaaaataat gaaaagatta tctataaccg tccgtttaac
      661 cttgcttttt atattgctgc tgtttgttgc tggcgctgga attgtctgga ctctctataa
      721 tggcctggca agtgagttga aatggcgcga tgatacaaca ctcattaacc ggacagcgca
      781 gatcaagcag ttgttaattg atggggtaaa tccagatacg ttacctgtgt actttaaccg
      841 gatgatggat gttagtcagg atatcttgat cattcatggt gatggcatca ataaaattgt
      901 taaccggaca aatgtcagta atggcatgtt aaataacata cctgctagtg agacaatcag
      961 cgcagctggc atttacagaa gcattattaa tgacacagag atagatgctt tacgaattaa
     1021 tattgatgaa gtttcgccat cattaacggt tactgtggct aaattggctt cagccagaca
     1081 taacatgctt gaacagtata aaattaatag cattataatt tgcattgtcg ccattatact
     1141 gtgctcagta ttaagtccgt tgttaatcag aacggggtta cgagagatca aaaagttgag
     1201 tggtgtaacg gaagcgctga attataacga tagccgagag cctgttgagg ttagcgcatt
     1261 accgagagaa ctaaaacctc ttgggcaggc gttgaataaa atgcaccaag ctttagtcaa
     1321 agattttgaa cgtctaagtc agtttgctga cgatctcgct catgaactta gaacgccaat
     1381 taatgcatta ctgggtcaga atcaggttac gctcagtcaa accagaagta tcgctgaata
     1441 tcaaaaaaca attgccggaa acattgaaga gctggaaaat atttcgcgat taacagagaa
     1501 catactgttt cttgccaggg cagataaaaa taatgttttg gtgaaactgg actcgctttc
     1561 tctcaataat gaagtcgaaa atttgttgga ctatcttgaa tacctttcag acgagaaaga
     1621 gatttgcttt aaggtcgagt gcaatcagca catctttgcg gataaaattt tactacaacg
     1681 aatgttatcg aatcttattg ttaacgctat tcgatattcg ccagaaaaat cgcgtattca
     1741 gataaccagt tttcttgata ccaacggttc tcttaatatt gatatcgcca gtcctggaac
     1801 gaaaattcat gagcctgaaa aactcttccg tagattttgg cggggagata attcgcgtca
     1861 ttccgtaggt cagggacttg gtctttcttt agtcaaagcg attgccgaat tacatggggg
     1921 aagtgctacg tatcactatc tcagtaagca taatgtgttc cggattacgt taccgcaaag
     1981 aaattaacat agggcttcag tacgccataa attaaccgtg aaaaacgcag ccacgtcgtg
     2041 agtactaacg cggctgcgat tgattatgcg cttacattca aacgtaacag ggattaaccc
     2101 gcgtaagctg ccagcatttc ctgtgccgcc agtttaccca acgcattcgc tgcgaaagga
     2161 ctgtcgccgg tgagaacttt acggtcctta tgtactcgcc cggtgatgtc gtcattaatg
     2221 atgttcatgc ccattttctt cagctcttcg ccgaagtacc aggtgagatg acccggcata
     2281 tagcctatct ctggcgtttg tttgtctgcg gcgtccggga atgcacaaat ggaataaccg
     2341 ttcagtgggt tatcgccgtg gcgaagcgcc agaaaagcag ccggaccatg gcatagggag
     2401 ataacaaaac ggtcattttc aatagcccac tgcagagcgg cggctacgtc ttcactttct
     2461 ggcaaaccga tgagtgcacc atgaccaccg ggaacaaaga tcgctgcata ttcgctgtct
     2521 gcgttcaggc tggcgacaac atccgctaat ttcttcggat tgcggaacaa cgatttatgc
     2581 tgctcaaaga atggcattac tttttcatct ttgtgcggca tagcccagta ttcaaacttg
     2641 gtcatcagac cggaaatggt cgccacttcg aattcgaaac ctgcagcatg gagatgatac
     2701 aacggcagca acgtttcaat cggatgattg cccgttgaga agagtttgcc gttatcagtc
     2761 ggcaaatagc gttcatcagc ggcgatcacc agaattttat gtttaccgcg atacggtttt
     2821 ggatagtcca cgccatcaag atcagagacg ggactcgtat attggctaag cgaatattct
     2881 gaagggaaga atgcattatc ttcagcaata tcgacctgtg gatttttact tttttgaaca
     2941 gtcatagtgt attccttatt gttgcttagt tagggtagtc actatatttg cgactgagct
     3001 ggccgctatt tttaaccagg ctggaaaatt gagtcaatat atcgaaagcg gtagtcggcg
     3061 ggcgtaagta tgtaatgatg atgctgcact ggtgttgaat attattcacg ccaaattttc
     3121 cttattgagc aacgccgtcg caagtggcct caatgatttt agaccattat cagaggtacg
     3181 gggcggtagg gagagattta gttatgtgga atattatggt gctgatgaat atttatatcc
     3241 cggattggta gggaaaaagc ttttttaatg cactattttt gcaaatattt ctatatggga
     3301 agcggttttg ttacagatta aatttataaa gaaatgcatt tgggtgttgg ctggaagaga
     3361 cgctggtaga ttttattgag ccaggggggg gcggggtaag gtgctgtatc gatatgcttt
     3421 ggcaattgcg tgtgccgcag atcgagatga aatgccaata aaaaagcctg agttcgatga
     3481 gggacgaact caggctagtg ttaagtttta cagtaacacg ccgcggcgcg ttggctgatt
     3541 agaactggta gacgatacca actgcaaact ggtcatcagc tgctgcgcca gtaatggcgg
     3601 cgtcatcttt atccagcatg ttgaatttgt atgcagtata aacgttcatg ttcttattga
     3661 agtagtacca ggtaccaact tcaatgtagt tcacacggtc agcgtcaaca tatctatgtc
     3721 caggtacacc tttgatatct ttacctttag aatacacata acctaaggac ggacgcagac
     3781 caaagtcgaa ttgatactga acaaccgctt caaagttctg ggtttcattg ataagttgat
     3841 cacgcattat tgtattgcct tctcgatcgg caacacctgt atcaatagaa cccggagtca
     3901 tattacgagt ttcaccgtac attacagctg cgtagacatt gttcgcgtca tactttgcag
     3961 ccaatgacca cagttcagca tttgaaccgt ttccatcggc aacttgatcg ttggtgcgat
     4021 cagatttgct atatgcacca acgataccaa aaccattgat gttatagtct acagacaacg
     4081 catagccgtc accattttgt ttcttagtgc tacggttgct gtttttgcct tggtactgaa
     4141 tcgcaaaatc aagaccatca accaggccaa agaaatcata gttacggtag gttgcaacac
     4201 cgttggtacg accattcatg aagttgtcag cggaagccca ggaatcgcca ccccattcaa
     4261 ctaacatatc ggtataagct gcggcatcat aagcaacacc gtagttacgg ccatagtcaa
     4321 atgatcctaa ctcgttatat ttcaaaccgg cgaaggttaa acgggttttt tcctggtttt
     4381 caccttcagg tttgctggcg tcgaggttgt attcaaactg accgaaacca gtcagttcgc
     4441 tggtgatttg ggtttcacct ttaacaccga aacgagcata ggaggtatct tcgttttcgc
     4501 tgttattatc atcagtattt gaccagatgt gttcgccaac catcttgccg tagaaatcaa
     4561 ctttattgcc atctttatta taaatttcag ccgcatttgc tgcgccagca actaataacg
     4621 ccgggaccag cattgccaga acttttcttt tcattatgta ttcccttgtg attataatct
     4681 tcatgaatat atcaataagt gccgttatcc aaaaaaaagc acatttggat actattctat
     4741 gaagttcatt ttattttaaa gattacatgt aacaaatgta tttaaaagat ttcaaaatgt
     4801 ttctaatcta tttaatataa ttcatagtta tgaa
//
LOCUS       DAZFFE010000195         4675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFFE010000195 DAZFFE010000000
VERSION     DAZFFE010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.196"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..574
                     /locus_tag="ACHJ69_004697"
     CDS             <1..574
                     /locus_tag="ACHJ69_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO5017501.1"
                     /translation="CKEWFHPAFSNQWWCCPEHGTQLALERRSKEREKAEKAAEKKRR
                     REEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISCGTLTSAQWDAG
                     HYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIGQEAVDEIESNHNR
                     HRWTVEECRAIKAEYQQKLKDLRNSRSEAT"
     gene            618..1106
                     /locus_tag="ACHJ69_004698"
     CDS             618..1106
                     /locus_tag="ACHJ69_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO5017502.1"
                     /translation="MGIRELNLTKEQHDWLNGWLELWGAWVYSGRLEKRMSSVIAKFM
                     ESVEPGRVMTRPMCNDDDGMLISQVVDSVMYIDKKAFGILLSYYAHGSSRHAIASYYH
                     RVARPRKMLCRGGGRIQKPSLATCRREVDEILNASLFMIYPVLDSAFKNRKRVEKIKH
                     VA"
     gene            1198..1272
                     /locus_tag="ACHJ69_004699"
     tRNA            1198..1272
                     /locus_tag="ACHJ69_004699"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1231..1233,aa:Asn,seq:gtt)
     gene            1278..1353
                     /locus_tag="ACHJ69_004700"
     tRNA            1278..1353
                     /locus_tag="ACHJ69_004700"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1311..1313,aa:Thr,seq:tgt)
     gene            1357..1431
                     /locus_tag="ACHJ69_004701"
     tRNA            1357..1431
                     /locus_tag="ACHJ69_004701"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1390..1392,aa:Gly,seq:tcc)
     gene            complement(1496..1650)
                     /locus_tag="ACHJ69_004702"
                     /pseudo
     CDS             complement(1496..1650)
                     /locus_tag="ACHJ69_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1721..2056)
                     /locus_tag="ACHJ69_004703"
     CDS             complement(1721..2056)
                     /locus_tag="ACHJ69_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO5017503.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            2317..4278
                     /locus_tag="ACHJ69_004704"
     CDS             2317..4278
                     /locus_tag="ACHJ69_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5017504.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVKPSDGEGTVISTTSEPEYYFVVALAGQSNGMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGASCNYNDIIPADHCLHDVQDLSKFSHPKASAAQYGCVG
                     QGLHIAKKLLPFIPANAGILLVPCCRGGSAFLAGDEGTFSESTGASETSARWGVDKPL
                     YKDLLTRTQAALKANPKNILLAVVWMQGEFDLKQGAYATQPGLFDSMVEKYRSDLSEF
                     GGQCLGGSPSSVPWICGDTTYYWKQTYSSQYDAVYGAYKTKSAKKIFFVPFMTDENGR
                     NVGTNEPSEDPDVADIGYYGAGGRTDAKTWTTADRKTHFGSWARRGIISDRLATAILV
                     HAGRTAEFITGKQPDTVKPTGPSGEGTEREPEAPVSNRTLMSLLASGEDLASQGWRYY
                     HKPASGDNVNKNIAEAVVSDAGATGGKALQLNKPENHIWFLEHDAAGQGVELLKKGGR
                     VSVRFKLPGSLVPNRFAMGIYWQLSSLPEGVTLAEEGNDMLMSFFLQTDATNLNAMYH
                     KKPNAKLDTFGVFDNGWHTLAFEFAGNNSIQVTPVLDEKRGTPFTLVKSPASGAADKL
                     QLTGILKAATYTLLIDSVKVEVNNADAAA"
     gene            4414..4608
                     /locus_tag="ACHJ69_004705"
     CDS             4414..4608
                     /locus_tag="ACHJ69_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO5017505.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGAKTEAPLIPEQPS"
BASE COUNT         1236 a         1030 c         1262 g         1147 t
ORIGIN      
        1 atgcaaggaa tggtttcacc cggcattctc aaatcagtgg tggtgctgcc cggaacacgg
       61 aactcaatta gcactcgaac gacgaagcaa agaacgagaa aaagcggaaa aagcagcaga
      121 gaagaaacga cgacgagagg agcagaaaca gaaagataaa cttaagattc gaaaactcgc
      181 cttaaagccc cgcagttact ggattaaaca agcccaacaa gccgtaaacg ccttcatcag
      241 agaaagagac cgcgacttac catgtatctc gtgcggaacg ctcacgtctg ctcagtggga
      301 tgccgggcat taccggacaa ctgctgcggc acctcaactc cgatttgatg aacgcaatat
      361 tcacaagcaa tgcgtggtgt gcaaccagca caaaagcgga aatctcgttc cgtatcgcgt
      421 cgaactgatt agccgcatcg ggcaggaagc agtagatgaa atcgaatcaa accataaccg
      481 ccatcgctgg actgtcgaag agtgcagggc catcaaggca gagtaccaac agaaactcaa
      541 agatctgaga aacagcagaa gtgaggccac atgacgctct cagtaaaaac cattccagat
      601 aacaagggag aagtcgcatg ggcataagag aactaaacct caccaaagaa cagcacgatt
      661 ggctgaatgg ctggcttgaa ctgtggggcg catgggttta ttcaggtcgt ctggaaaagc
      721 gcatgagcag cgtaatagcg aagttcatgg agagcgtaga gccgggaaga gttatgacaa
      781 gaccaatgtg caatgatgat gatggaatgt tgatttctca ggtcgtcgat tccgtcatgt
      841 acattgacaa gaaagccttc ggcatcctcc tcagctacta cgctcatggt tcttccaggc
      901 acgctattgc atcttactat catcgcgtcg caagacctcg caagatgtta tgccggggcg
      961 gcgggcgcat tcaaaaacca tcgctcgcaa cctgtcgacg ggaagttgac gaaatcctta
     1021 atgcctcgtt gtttatgatt tacccggttc tggatagtgc gtttaaaaac cggaaacgtg
     1081 tagagaaaat taaacatgta gcatagaacg tgttgacatc attgagcaaa tgagcaacac
     1141 tattggcata agctgccgtt agtgactctt aagttgcaac ggtggctttt tttatttggg
     1201 tcagtcgtat aaaggtcatt acggaaggct gttaaccttc ttatcgtggt tcgagtccac
     1261 gctgtcccgc caaacatgct ggtttagctc caatggtaga gcaactgact tgtaatcatc
     1321 aggtcgccag ttcgattccg gtagccggca ccatatgcgg gtaccgtata atggctatta
     1381 cctcagcctt ccagtctgat gatgcgggtt cgattcctgc tacccgctcc agatttatta
     1441 tcaggctcgc ttcggcgggc ctttttttta aatgtctcac aattcagacg gttgacagtt
     1501 gtctgttttg cggggaattt gttaaaagaa actggcatgg tgaatccccc tgtgcggagg
     1561 ggcaatcagc gagtaggtat atgggataat cgcggattca ggtgctggta ctgaattcac
     1621 cgggaggcac ccggcaccat gcaatggcac atagcgccac tctccagccc ctctccggag
     1681 gggctgttta tattgatttt gtcagatgtg agtaaactcc ttatggactt tgttgtttta
     1741 gtccataagg acatatttgc agagtgcaac ggttattaaa gcattcattc aatacgttat
     1801 ctgtatttgt agggcattcc tggctgtttt tgattaaatt ccagaatgtt ttattgaatg
     1861 gtactacgtt gtaaatggtt acaggtagca ctttgttatt gagcatgata cctgtgtgag
     1921 tcagtgtaaa tatactttca ggaggtaaga aagcatccga ttgataccag attattaatt
     1981 ttattttact ccatatgact gaaaaagata ttccgcatga tggctggata actgtatcaa
     2041 tcacaatcca cttcatttag tttccttgtt tatgtcttgc tggtgatgtt ctgaaaagta
     2101 taaatgatat ttttgaatgt aaaccataga gcagaattat ttttctgatg ttgtttattg
     2161 tttatttaaa tgcagggtgg tttatatctc gtcttgtagt ttacccatgc atatctgctt
     2221 gatgatgagg tttttattta aggtatggtt ttgtgttttt tctgtattac atgtcaggta
     2281 ttttaaagaa ttatttttca gatggtggaa agaaccatgg catttaaaca ctatgatgtt
     2341 gtcagggcgg catcgccgtc agaccttgcg aaacgactga cacaaaaact gaaggagggg
     2401 tggcagccat ttggcagtcc ggtggccatc acgccctata ccctgatgca ggccattgcg
     2461 gcggaaggtg atgtcaccac acctgtggtg gtgaagccgt cggatggaga aggcacagtt
     2521 atcagcacca ccagcgaacc ggagtattac tttgttgttg ccctggccgg gcagtcaaac
     2581 ggtatggcgt atggtgaagg gcttccgctg ccggagacat atgaccgtcc ggacccgcgt
     2641 attaaacagc tggcgcgtcg cagcactgtc acgccgggtg gtgcgtcctg taactacaat
     2701 gacattattc ctgcggacca ctgcctgcat gatgttcagg atttgagtaa gttttcacac
     2761 ccgaaagcca gcgcagctca gtatggatgc gtggggcagg gattacatat cgcgaagaaa
     2821 ttgttgccgt ttattccggc gaatgccggt attcttctgg ttccgtgctg ccgtggtggt
     2881 tctgcatttt tggcgggcga tgaaggtacc ttcagcgaat ccaccggcgc aagcgagacc
     2941 tcggcacgtt ggggtgtaga taagccactg tacaaggacc tgcttacccg tactcaggcc
     3001 gcactgaagg ccaaccctaa aaatattctg cttgcagtgg tctggatgca gggcgagttt
     3061 gatttgaaac agggtgcata cgccactcag ccggggctgt ttgattccat ggtggaaaaa
     3121 tatcgttctg acctgtcgga attcggaggt cagtgtctcg ggggctctcc gtcatcggtt
     3181 ccctggattt gtggcgacac gacctactac tggaagcaga cttattcttc gcaatacgat
     3241 gcggtgtatg gtgcatacaa gacgaaatcc gcaaaaaaaa tcttctttgt gccgtttatg
     3301 acggatgaaa acgggcgaaa tgtgggtacc aacgagccgt cagaagatcc ggatgttgcg
     3361 gatattgggt attacggagc cggtggtcga acggacgcca aaacctggac gacggccgac
     3421 cgtaaaacgc attttggatc atgggcacgt cgtgggatta tttccgaccg tctggcaacg
     3481 gcgattcttg tgcatgccgg gagaaccgct gaattcatta ccggaaaaca gcctgatacg
     3541 gtgaagccca ccggaccttc cggtgaaggt acggagagag agccggaagc cccggtcagt
     3601 aaccgaaccc tgatgagtct gctggcgtcc ggcgaagacc tggcatcaca gggctggcgc
     3661 tattatcaca aaccggcgag cggagacaat gttaacaaaa acattgctga agcggtggtc
     3721 agtgatgcgg gggctacggg aggtaaggcc ctgcaactga ataaaccgga aaaccacatc
     3781 tggtttctgg agcatgatgc agccggacag ggagtggagt tactgaagaa ggggggacgt
     3841 gtgagcgtac ggtttaagtt gccgggttca ctggtgccga atcggtttgc catgggcatt
     3901 tactggcagt tgtcgtccct gccggaggga gtgacgctgg cagaggaagg caacgacatg
     3961 ctgatgtcct tcttcctgca gacggatgcg acgaacctga acgcgatgta ccacaagaag
     4021 ccgaatgcga agctggatac gttcggggtc tttgataacg gatggcacac actggctttt
     4081 gagtttgccg gaaacaacag cattcaggtg acgccggtac tggatgagaa acgggggacg
     4141 ccgttcacac tggtgaaatc tccggcatca ggggcggcgg acaaactgca actgacaggc
     4201 atattaaagg cggcgacata tacgctgctg attgacagtg tgaaggtgga agtgaacaac
     4261 gcggatgccg cggcatgata aaaaaagccg ccagcggcag gaatggaagc tggcggaggt
     4321 aatcccaatg gagaatgtaa agaaaagatg ctttcgtata tcggtctttt aaatgaaaac
     4381 agttctcatt gtcaaccata acggtaataa actatgacat ttattcatca ggtgatgctg
     4441 tacttctgta cggcggtctg tgtgctgtat cttctttcgg gtgggtacag ggcagtgcgc
     4501 gatttctggc gcaggcagat tgataaaagg gccgcagaga aaatcagcgc cagtcagtca
     4561 gccggagcaa aaacagaagc cccactcatt ccggaacaac cttcttaata acccctttca
     4621 acgagaaaat cctatgtcag aaataaaatc gctggtcact gctgaggcag tgaag
//
LOCUS       DAZFFE010000196         4555 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFFE010000196 DAZFFE010000000
VERSION     DAZFFE010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.197"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..847
                     /gene="yibB"
                     /locus_tag="ACHJ69_004706"
     CDS             <1..847
                     /gene="yibB"
                     /locus_tag="ACHJ69_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFO5017506.1"
                     /translation="TTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLAALEN
                     EMIIFTSPDLKSRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRLEPRQ
                     LKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDF
                     PFDENKMHLFTIKKWLAVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLVLESQ
                     KITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRI
                     FLSRK"
     gene            1122..2318
                     /gene="kbl"
                     /locus_tag="ACHJ69_004707"
     CDS             1122..2318
                     /gene="kbl"
                     /locus_tag="ACHJ69_004707"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO5017507.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLSAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            2328..3353
                     /gene="tdh"
                     /locus_tag="ACHJ69_004708"
     CDS             2328..3353
                     /gene="tdh"
                     /locus_tag="ACHJ69_004708"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO5017508.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            3592..>4555
                     /gene="waaH"
                     /locus_tag="ACHJ69_004709"
     CDS             3592..>4555
                     /gene="waaH"
                     /locus_tag="ACHJ69_004709"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO5017509.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIRFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSF"
BASE COUNT         1171 a          971 c         1162 g         1251 t
ORIGIN      
        1 caccacaatt atcactgcat attttgacat tggtagaggg gattggactg ctaataaagg
       61 gttccgtgaa aaactagctc gttcagttga tgtttatttt agttactttg agcgtctggc
      121 cgcacttgaa aatgaaatga ttattttcac ttctcccgat ttgaaatcaa gggttgaagc
      181 gattcgcaac ggaaaaccaa caacggttat cgttatcgat ataaaaaaga aatttagata
      241 tatcagaagt cgtatcgaaa aaattcaaaa agatgagtcg tttacaaata gacttgagcc
      301 gcggcaatta aaaaacccag agtactggtc accagagtat gtattggtat gtaaccttaa
      361 ggcctatttt gtaaataaag ctatcaacat gggcttagtg aaaactcctc ttgttgcgtg
      421 gatagatttt ggctattgcc gtaagccaaa tgtaactcga ggattgaaaa tttgggattt
      481 cccatttgat gaaaataaga tgcatctttt cactataaaa aaatggcttg ctgttacatc
      541 tcaacaacag gcttttgatt tcatgattgg caatcatgta tatattattg gtggcgcaat
      601 tgttggttca cagcataaat ggaaagaatt ctataaatta gttctggaaa gtcaaaaaat
      661 aaccctaaac aataacattg tggacgatga ccagggcatt tttgtcatgt gctattacaa
      721 acgttccgat ctcttcaacc taaactatct ggggagagga aaatggttcg atttgtttcg
      781 ctgcttcagg agtaacactt taggggcaaa aatgcaggcg ctgagaattt tcctttcaag
      841 aaaataggtt tccctccatt agtgggtagc gttttgatac caaatgagat tggaaaaatg
      901 atgctctggc atcatttttt ctttttatat caatgcagta aaatcactca tcattatgtc
      961 cagccaaaac tgtacagttt ttctactatc tgtaggtgtt aagagacatc aagatacacg
     1021 ttatttatcc tgaattttgc agaagtgtta acgcgtcatc tcgtcgcgac ctataagttt
     1081 gggtaatatg tgctggaatt tgccctgtct ggagaatcgc aatgcgtgga gaattttatc
     1141 agcagttaac caacgatctg gaaaccgcac gggcggaagg gttgtttaaa gaagagcgca
     1201 ttattacgtc tgcgcagcaa gcagatatca ctgtggctga tggaagccac gtcattaact
     1261 tttgtgccaa caactatctc ggactggcga atcatccgga tctgattgcg gcggcaaagg
     1321 cgggaatgga ttctcacggt ttcggcatgg cttcggtgcg ttttatttgc ggcactcagg
     1381 acagccataa agagcttgaa caaaaactgg cggcattcct ggggatggaa gatgcgattc
     1441 tctactcttc ctgctttgat gctaacggcg gcctgtttga aacgctgctg agcgcggagg
     1501 acgccattat ctccgacgca ctgaaccacg cgtctattat tgatggtgtg cgtctgtgta
     1561 aagctaaacg ctatcgttat gccaacaacg atatgcagga gctggaagca cgtctgaaag
     1621 aagcgcgtga agccggtgcg cgtcatgtgc tgattgccac cgatggtgtg ttctcgatgg
     1681 acggcgtgat tgccaacctg aagggcgttt gcgatctggc agataaatac gatgccctgg
     1741 tgatggtaga cgactcccac gcggtcggtt ttgtcggtga aaatggtcgt ggttcccatg
     1801 aatactgcga tgtgatgggg cgtgtagaca tcattaccgg tacgttgggt aaagcattgg
     1861 gcggggcttc tggtggttat accgcggcgc gcaaagaagt ggttgagtgg ctgcgccagc
     1921 gttctcgtcc gtacctgttc tccaactcgc tggcaccggc gattgtcgcc gcatccatca
     1981 aagtgctgga gatggtcgaa gcgggtagcg agctgcgtga ccgtctgtgg gcgaacgcgc
     2041 gtcagttccg tgagcaaatg tcggcggcgg gttttaccct ggcgggagcc gatcacgcca
     2101 ttattccggt catgcttggc gatgcggtag tggcgcagaa atttgctcgt gagctgcaaa
     2161 aagagggcat ttacgtcacc ggtttcttct atccggtcgt tcctaaaggt caggcgcgta
     2221 ttcgtaccca gatgtctgcg gcgcataccc ctgagcaaat tacgcgtgca gtagaagcat
     2281 tcacgcgtat tggtaaacaa ctgggcgtta tcgcctgagg gtgtgagatg aaagcgttat
     2341 ccaaactgaa agcggaagag ggcatctgga tgaccgacgt tcctgtaccg gaactcgggc
     2401 ataacgatct gctgattaaa atccgtaaaa cagccatctg cgggactgac gttcacatct
     2461 ataactggga tgagtggtcg caaaaaacca tcccggtgcc gatggtcgtg ggccatgaat
     2521 atgtcggtga agtggtaggt attggtcagg aagtgaaagg cttcaagatt ggcgatcgcg
     2581 tttctggcga aggccatatc acctgcggtc actgtcgcaa ctgtcgtggc ggtcgtaccc
     2641 atctgtgccg caacacgatc ggcgtcggtg ttaaccgtcc gggctgcttc gccgagtatc
     2701 tggtgattcc ggcgttcaat gccttcaaaa tcccggacaa tatctccgac gacctggctt
     2761 ccatttttga tcccttcggt aacgctgtgc ataccgcgct gtcgttcgat ctggttggcg
     2821 aggatgtgct ggtctctggt gcaggtccga taggtattat ggctgcggcg gtggcgaaac
     2881 acgttggtgc acgcaatgtg gtgatcactg atgttaacga ataccgcctt gagctggcac
     2941 gcaaaatggg tatcacccgt gcggttaacg tcgctaaaga aaaccttaat gatgtgatgg
     3001 cagaactggg catgaccgaa ggctttgatg tcggtctgga aatgtccggt gcgccgccag
     3061 cgtttcgtac catgctcgac accatgaacc acggcggtcg tattgcgatg ctgggtattc
     3121 caccgtccga tatgtctatc gactggacca aagtaatctt taaaggcttg ttcattaaag
     3181 gtatttacgg tcgtgagatg tttgaaacct ggtacaagat ggcggcgctg attcagtctg
     3241 gcctcgatct ctcgccgatc attacccatc gtttctctat cgatgatttc cagaagggct
     3301 ttgatgccat gcgttcgggc cagtccggga aagttattct gagctgggat taacacgaac
     3361 aagggctggt attccagccc ttttgcctga ggataatccg ttaaatatgt aaaatcctgt
     3421 cagtgtaatc aagatatcgt aattgcactg atctcttata tagctgctct cattatctct
     3481 ctaccctgac gtgactctct caccggtaaa aataatatct cacaggctta atagtttctt
     3541 aatacaaagc ctgtaaaacg tcaggataac ttcagaggtc gtcggtaatt tatgatgaac
     3601 agcaccaata aacttagtgt tattattccg ttatataatg cgggcgatga tttccgcact
     3661 tgtatggaat ctttaattac gcaaacatgg actgctctgg aaatcattat tattaacgat
     3721 ggttcaacgg ataattctgt tgaaatagca aagcattacg cagaaaacta tccgcacgtt
     3781 cgtttgttgc atcaggcgaa tgctggcgca tcggtggcgc gtaatcgtgg gattgaagtg
     3841 gcaacgggaa aatatgtcgc ttttgtcgat gctgacgatg aggtctatcc caccatgtac
     3901 gaaacgctga tgaccatggc gttagaggac gacctcgacg tggcgcagtg caacgctgat
     3961 tggtgttttc gtgaaacggg tgaaacctgg caatccatcc ctaccgatcg ccttcgctca
     4021 accggtgtat taactggtcc ggactggctg cggatgggac tttcttcccg ccgttggaca
     4081 cacgtggtct ggatgggggt ttatcgccgc gatgttattg ttaaaaataa cattagattt
     4141 attgccggat tacatcatca ggatattgtc tggacaacag aatttatgtt taacgcgctg
     4201 cgtgcgcgat ataccgagca atcattatat aaatattatc tgcataatac gtcagtgagt
     4261 cggttgcata gacaagggaa taaaaacctt aattatcaac gtcactatat taagattacc
     4321 cgcctgctgg agaaattaaa tcgaaattat gccgacaaaa ttacgattta tccggaattt
     4381 catcagcaaa taacttacga agcattgcgt gtttgccatg cggtgcgcaa agagccggat
     4441 attattaccc gccaacggat gattgccgag atatttactt ccggtatgta taagcgcctg
     4501 attactaatg tgcgcagcgt gaaggtgggt taccaggcgt tactgtggtc tttcc
//
LOCUS       DAZFFE010000197         4379 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFFE010000197 DAZFFE010000000
VERSION     DAZFFE010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.198"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1083
                     /gene="ompC"
                     /locus_tag="ACHJ69_004710"
     CDS             <1..1083
                     /gene="ompC"
                     /locus_tag="ACHJ69_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO5017510.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTDGQ
                     VAYGKSKFNASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEA
                     VAQYQFDFGLRPSVAYLQSKGKDLGVHGDRDLVKYVDVGATYYFNKNMSTFVDYKINL
                     IDDSKFTKTAGIDTDDIVAVGLVYQF"
     gene            complement(1272..1655)
                     /gene="quuD"
                     /locus_tag="ACHJ69_004711"
     CDS             complement(1272..1655)
                     /gene="quuD"
                     /locus_tag="ACHJ69_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO5017511.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSVAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVCGMTFMSLASKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLDMDIVANNSN"
     gene            complement(1741..1881)
                     /locus_tag="ACHJ69_004712"
     CDS             complement(1741..1881)
                     /locus_tag="ACHJ69_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO5017512.1"
                     /translation="MMFESYMAERLRRRWVRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(1878..2240)
                     /gene="rusA"
                     /locus_tag="ACHJ69_004713"
     CDS             complement(1878..2240)
                     /gene="rusA"
                     /locus_tag="ACHJ69_004713"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO5017513.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYCDNVARIIKN
                     AMLDIGLAMPVKISIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGDE"
     gene            complement(2237..2527)
                     /locus_tag="ACHJ69_004714"
     CDS             complement(2237..2527)
                     /locus_tag="ACHJ69_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO5017514.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAGYAKECALEGMARTQVIWLKEGVIEA"
     gene            complement(2520..2690)
                     /locus_tag="ACHJ69_004715"
     CDS             complement(2520..2690)
                     /locus_tag="ACHJ69_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO5017515.1"
                     /translation="MATPLIRVMNGHIYKVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(2690..3145)
                     /gene="ybcN"
                     /locus_tag="ACHJ69_004716"
     CDS             complement(2690..3145)
                     /gene="ybcN"
                     /locus_tag="ACHJ69_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO5017516.1"
                     /translation="MNFPQDGIKLHRGNFTAIGRQIQPYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLISRGKTFATPAWVKDALKHTYLGYETKELVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(3142..3243)
                     /locus_tag="ACHJ69_004717"
     CDS             complement(3142..3243)
                     /locus_tag="ACHJ69_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017517.1"
                     /translation="MRTYNPTYLLPSQMQKCTCDILHPAFDLCGGEA"
     gene            3593..>4379
                     /locus_tag="ACHJ69_004718"
     CDS             3593..>4379
                     /locus_tag="ACHJ69_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFO5017518.1"
                     /translation="MKINKILSSATLLYGMSMAMSVGSCATPVQTNLPGYTPGADIIS
                     VSPTRNQVDLIGDVVYSQIKGTRSVRQLHMSVLVPRTNDLKPAIIYYPGGGFMSSEHD
                     KFIEMRMALAEAGFVVAAVEYRTIPDTFPAPVEDGKAAIRYLREHASNYGIDPQRIGV
                     LGDSAGGWLAQMMGTTNGDKTFDKGDFLQQSSDVQAVATLYGISDLLNIGEGFPESVQ
                     EVHRSPAVTEALMINGPAFRNFAGAPITASKEKALNASPIGHMK"
BASE COUNT         1193 a         1029 c          969 g         1188 t
ORIGIN      
        1 atgaaaaaat taacagtggc aatttctgct gtagctgcat cagtactgat ggcgatgtct
       61 gctcaggcag ctgaaattta taataaagac agtaacaagc tggatctata cgggaaagtt
      121 aatgccaagc actacttctc ctctaatgat gcagatgatg gtgatactac ttatgcccgt
      181 cttggcttca aaggtgaaac ccaaatcaac gatcaactga ctggtttcgg tcagtgggaa
      241 tatgaattca aaggcaaccg tgctgaatct caaggttctt ccaaagacaa aacccgtctt
      301 gcatttgcag gcctgaaatt tggtgattac ggctcaatcg attacggccg taactacggt
      361 gtagcatacg acatcggtgc gtggactgac gtcctgccag aattcggtgg tgatacctgg
      421 acccaaacag atgtgttcat gactggtcgc actactggtg ttgcaactta tcgtaacaac
      481 gacttctttg gtctggtcga tggcctgaac tttgctgctc agtatcaggg taaaaatgac
      541 cgcactgacg taactgaagc caatggtgat ggtttcggtt tctccactac ttatgagtat
      601 gaaggattcg gcgtgggtgc aacctatgct aaatcagatc gcactgacgg tcaggtcgcc
      661 tatggtaaga gcaaattcaa tgcctccggc aaaaatgcgg aagtatgggc tgcaggcctg
      721 aaatatgatg cgaacaatat ctatctggct accacatatt ctgaaactca gaatatgacc
      781 gtttttggta ataaccatat tgcaaacaaa gcacaaaact ttgaagcagt agcacaatat
      841 cagtttgact tcggtctgcg accatctgtt gcttaccttc agtcaaaagg taaagacctt
      901 ggtgttcatg gtgaccgaga cttagtcaag tatgtcgatg tcggtgctac ttactacttt
      961 aataaaaaca tgtccacttt tgttgattac aaaatcaact taattgacga tagtaagttt
     1021 accaaaacag ctggtattga taccgacgac atcgtcgctg taggtctggt ttatcagttc
     1081 taatctgact tacgaaaaag atatgttgcg ggaggctttg cctccgcaac atataagtgg
     1141 agccctcaag ccacttcctt tagaagcact accttgcttc ttactatata aaccttctgt
     1201 tatatattac cctttatttt gggggcgttt ccacgcccca tttttaataa cttttagtaa
     1261 acaattgcat atcaattaga attattagca acgatatcca tatctaaccg gatatctaat
     1321 gccattaaca tcccttcaat tatgccctca gccttctgta accttttccc gatataacca
     1381 tccgagcagc aatgcttact tgccagtgac atgaatgtca taccacatac ataataatct
     1441 actaataaat cgtgtaaatc gctgttgttc tttttcagac gggccatgca cccgcaaatg
     1501 atcatcgcgt catcgtcaca acattgcggg cgagatttta cttttgaagg aattaatccc
     1561 ttaaaaccgg cggcaacgga cgaccaggtc acatcttcat gattattagc cgcccacgct
     1621 ccccaacgct caagaaccat ctgaatatca cgcatcaact tactccacaa aaatcagacc
     1681 agaacgccaa ttacaagcaa aaatcaacaa aacagtatta gttgattgtt atctctgact
     1741 tcatactcct gctcctgtca gggttttggc gtaattcttc agtattcggt aatcggtcaa
     1801 aacagaacca ggaaaacgat ataagcgcag gcgcacccag cggcggcgaa gacgctctgc
     1861 catataagac tcaaacatca ttcatctccc agttcagtga tagtcagctc cagcttccca
     1921 cctttggtaa cgggcatctt cacaacgcgg taatcaacga cctgagcatc atccagccag
     1981 aaacctgctt tggtgagtgc gtcaaaagcg gccttttgca gattatcaag gtcacggcga
     2041 cggcgatccg gcatgtggca ctcaatgctg attttcactg gcatagccaa gccgatatcc
     2101 agcattgcgt ttttaatgat tcgggcgacg ttatcgcagt atgcctgccc ctctgcgctg
     2161 acgtgcgtgc gcccacgatt atggcggtaa tagcgattat tgctcggagg ccagggtaat
     2221 gtgatgctgt aggtattcac gcctcaataa ccccctcttt caaccagata acctgtgttc
     2281 tcgccatacc ttccagcgcg cattcttttg catatccagc gtcaacaaaa tgcgtgcgac
     2341 ggtcgatttc gtcgtggcag gcagaacatg caatggtggc aatcaggtct ggcggtttga
     2401 taccggtacc gcacaatcca gccagccgga tatgtgccag tactgacgtt tcaggattgc
     2461 cattacatac gccagggatt cttacctggc attcccgacc acgcgctgct tttcttaaat
     2521 cagccatgat tcctccttgc tgccagtcgc aaccattttt tatcaaccag gctggcggta
     2581 tacccgagca gtgttggtat ttcggatggc ttcagctcag gtttacgctt acgacgattt
     2641 ggtactttgt agatgtgtcc gttcatgaca cgaataagcg gtgtagccat tacgcctcct
     2701 gcttgtcgcg cagcagctgg aactcgcagc tctgcggaat agtcaggtgg cagccaatat
     2761 tcatcgccca ggcttcaacc ttacacagga agacatacat ctctccggta tcgagatcgg
     2821 aggtatgacg taatgactga atggtggtga tttcaccggt tacgacatca accagttctt
     2881 tggtttcata accgagataa gtgtgtttga gagcatcttt tacccaagct ggagtggcga
     2941 acgttttacc cctgctgatg aggtattcac tgatttcgct gtaccacatg tggctgagtg
     3001 cattctggga aagactgcgt ctctcgcgcc atggtttaag caccatgcga aagcatttgc
     3061 cgtcctccag ataaggctgg atctgccgac cgatagcggt gaagttaccg cgatgcagtt
     3121 tgatgccatc ttgtgggaag ttcacgcttc acctccgcag agatcaaacg ctggatgcaa
     3181 gatatcgcag gtgcatttct gcatctgtga agggagaaga taggttggat tgtatgtgcg
     3241 cataaacgtc cccgtttagc gcagaagtca ccggagttgt tcaggctccg gtgatacaat
     3301 tatggcgaat tgattattca taatcaaaca agataaggtc tcaaacttca tgcaagccaa
     3361 gatttatttc tgacagaatt atacaaagga gctattggtc agaatctact cggactgtaa
     3421 aacatacgca taaccttaag ctctcacttt aagcattgtt gaaataatag ccgtcaagta
     3481 caaccttaac cacgactggg atatttccct ggctaccacg agttgtacgg ctattaaact
     3541 gccgttaaat tcagtaagag aatttcatcc gataagtcaa ggcatgtaaa acatgaaaat
     3601 taacaagata ttatcatctg caacactatt gtatggtatg tcaatggcca tgtcggtcgg
     3661 gagttgtgca acacctgtcc agactaatct tcctggttac accccgggtg cagatatcat
     3721 tagtgtttca ccgaccagaa accaggtcga tctcattggt gatgttgttt attcccagat
     3781 aaaaggaact cgttctgtca gacagcttca catgtcagtt cttgtcccgc gaacaaatga
     3841 tttaaaacca gccattattt attatcccgg cggcggattc atgtcttctg aacatgacaa
     3901 atttattgaa atgagaatgg ctctggcaga agctggtttt gttgtggccg ctgtagaata
     3961 cagaacaatt cctgatacat ttccagcacc agttgaggat ggaaaagctg caatacgtta
     4021 cctgagagag catgccagca attatgggat tgatcctcaa agaatcggag ttctgggtga
     4081 ctctgccggt ggatggcttg ctcagatgat gggaactaca aatggtgaca aaacctttga
     4141 taaaggtgac tttcttcagc aatcctcaga tgttcaggca gttgccacac tttatgggat
     4201 ttctgacttg ttgaatattg gcgaggggtt ccctgaatca gtgcaggagg ttcatcgctc
     4261 tcctgccgta accgaagcct taatgatcaa tggccctgca ttcagaaatt ttgcgggagc
     4321 ccccatcaca gcgtcaaaag aaaaagcgct aaacgccagt ccaatcggac atatgaaag
//
LOCUS       DAZFFE010000198         4378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFFE010000198 DAZFFE010000000
VERSION     DAZFFE010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.199"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..368)
                     /locus_tag="ACHJ69_004719"
     CDS             complement(<1..368)
                     /locus_tag="ACHJ69_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO5017519.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLG"
     gene            complement(483..558)
                     /locus_tag="ACHJ69_004720"
     tRNA            complement(483..558)
                     /locus_tag="ACHJ69_004720"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(523..525),aa:Thr,seq:ggt)
     gene            complement(565..639)
                     /locus_tag="ACHJ69_004721"
     tRNA            complement(565..639)
                     /locus_tag="ACHJ69_004721"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(604..606),aa:Gly,seq:tcc)
     gene            complement(756..840)
                     /locus_tag="ACHJ69_004722"
     tRNA            complement(756..840)
                     /locus_tag="ACHJ69_004722"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(804..806),aa:Tyr,seq:gta)
     gene            complement(849..924)
                     /locus_tag="ACHJ69_004723"
     tRNA            complement(849..924)
                     /locus_tag="ACHJ69_004723"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(889..891),aa:Thr,seq:tgt)
     gene            1286..2236
                     /gene="coaA"
                     /locus_tag="ACHJ69_004724"
     CDS             1286..2236
                     /gene="coaA"
                     /locus_tag="ACHJ69_004724"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO5017520.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2265..3230)
                     /gene="birA"
                     /locus_tag="ACHJ69_004725"
     CDS             complement(2265..3230)
                     /gene="birA"
                     /locus_tag="ACHJ69_004725"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO5017521.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEKILSQLDDGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQHAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLTPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3227..4255)
                     /gene="murB"
                     /locus_tag="ACHJ69_004726"
     CDS             complement(3227..4255)
                     /gene="murB"
                     /locus_tag="ACHJ69_004726"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO5017522.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETANALLAQFPTAPHYPQVDGSVKLAAGWLIDQCQLKG
                     TQIGGAAVHRQQALVLINEHDAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1125 a         1093 c          995 g         1165 t
ORIGIN      
        1 cccagcagga tgtgctcacg agtctgcggc atcgggccgt cagtcgcagc aactaccagg
       61 atcgcgccgt ccatctgagc agcaccggtg atcatgtttt taacatagtc ggcgtgcccc
      121 gggcagtcta cgtgcgcgta gtgacgggtc ggggtgtcgt attcaacgtg agaagtgttg
      181 atggtgatac cacgagcttt ttcttccggc gcgttatcga tctggtcgaa tgcgcgagca
      241 gcaccgccgt aggttttagc cagtacggta gtgattgcag cggtcagcgt tgttttacca
      301 tggtcaacgt ggccgatagt accgacgtta acgtgcggtt ttgtacgttc aaacttttct
      361 ttagacatcg attgtccctc taagacacgg ataaatcggt gatatcacca catcaaccag
      421 gcaacatgcc cgacttgttg aatgcaataa acagaaggaa aaaacaggga ggagaaaaga
      481 agtggtgctg ataggcagat tcgaactgcc gacctcaccc ttaccaaggg tgcgctctac
      541 caactgagct atatcagcac atcttggagc gggcagcggg aatcgaaccc gcatcatcag
      601 cttggaaggc tgaggtaata gccattatac gatgcccgca tcctggaact cggctacctg
      661 attttcattc tgcactgaat atcgagagaa gctctcttta ttcgagccgg taagcgaact
      721 tatcgtctcg ggctacgcca tcgcgtggcc gaaattggtg gtgggggaag gattcgaacc
      781 ttcgaagtct gtgacggcag atttacagtc tgctcccttt ggccgctcgg gaaccccacc
      841 ggacttgatg gtgccgacta ccggaatcga actggtgacc tactgattac aagtcagttg
      901 ctctacctac tgagctaagt cggcatcaag tagcgcgcat tctatggaga catgcgagtt
      961 catgcaacta aaaaattgca taatttgttt tattggtcac attttacgcg acacgatgaa
     1021 gaaacagccg aacggagggc agattagcac actttttcaa catcattgtg ctcaacaatg
     1081 tgctcctgct aaaccataat tctttttatc agatggaata tctgtcacat tgcttttcaa
     1141 cgatagcttc ctgggagaga ttttttctta ttattcctcc ccatctggtg ttaccctcct
     1201 gcccattaac ccattcaaca gaactgtgac gcgccatggc aaatatcgct ttgccgatag
     1261 agctatgacc gccagaaaca tgcttatgag tataaaagag caaacgttaa tgacgcctta
     1321 cctacagttt gaccgcaacc agtgggcagc tctgcgtgat tccgtaccga tgacgttatc
     1381 ggaagatgag atcgcccgtc tcaaaggtat taatgaagat ctctcgttag aagaagttgc
     1441 cgagatctat ttacctctgt cacgtttgct gaacttctat ataagctcga atctgcgccg
     1501 tcaggcagtt ctggaacagt ttcttggtac caacgggcaa cgcattcctt acattatcag
     1561 tattgctggt agtgtcgcgg tggggaaaag tacaaccgcc cgtgtattgc aggcgctatt
     1621 aagccgttgg ccggaacatc gtcgtgttga actgatcact acagatggct tccttcaccc
     1681 taatcaggtt ctgaaagaac gtggtctgat gaagaagaaa ggcttcccgg aatcgtatga
     1741 tatgcatcgc ctggtgaagt ttgtttccga tctcaaatcc ggcgtgccaa acgttacagc
     1801 ccctgtttac tcgcatctta tttatgatgt gatcccggat ggagataaaa ctgttgttca
     1861 gcctgatatt ttaattcttg aagggttaaa tgtcttacag agcgggatgg attatccaca
     1921 cgatccacat catgtatttg tttctgattt tgtcgatttt tcgatatatg ttgatgcacc
     1981 ggaagactta cttcagacgt ggtatatcaa ccgttttctg aaattccgcg aaggggcttt
     2041 tacagacccg gattcctatt ttcataacta cgcgaaatta actaaagaag aagctattaa
     2101 gactgccatg acattgtgga aagagatcaa ctggctgaac ttaaagcaaa atattctacc
     2161 tactcgcgag cgtgccagtt tgatcctgac gaaaagtgct aatcatgcag tagaagaggt
     2221 cagactacgc aaataatttt caggggagct aatgctcccc tttcttattt ttctgcacta
     2281 cgcagggata tttcaccgcc catccagggt tttattattc catcctgctc aagtaataaa
     2341 gcaccctgtt tgtctattcc gcgtgaaatg ccaaatattt ctttatcacc aatgataagt
     2401 ttcactgggc gattaataaa attatccaac ttttcccagc gcgaaagata aggtgtcaat
     2461 ccttcttgct cgaagagttc cagcgccgcg cgtaattcac gtatcagcat ggccgcaaga
     2521 gtattacgat cgagattgat cccggcttcc tgcagcgtga tccacccttg attaacgaca
     2581 ctctcttcaa cacgacgcat tgccatgttg atccctgcac caatgactat ttgcgccgca
     2641 tcgccggttt tgccagtcag ctccacaaga atgcctgcca gcttacgatc ctgcagatag
     2701 aggtcattag gccatttaac acgaacttta tctgcaccca gcttgcgtaa tacttccgcc
     2761 atcacgatac cgataaccag acttaaacca atcgccgccg ccggaccctg ttccagacgc
     2821 cagaacatcg acaaatataa gttcgcgcca aaaggcgaaa accatttccg accacgacga
     2881 ccacggccag catgctggta ttctgcaaca caggcatcgc cagatttaag ctctccgatg
     2941 cggtccagga gatactgatt cgtggagtca ataactggca gcacggctac actgccgtca
     3001 tccagctgac tcaatatctt ttcggcatta agtaactgga ttggctcggg caagctgtat
     3061 cctttacccg gaacagtaaa tacatcaacg ccccagtcac gcagtgtctg aatgtgttta
     3121 ttaatagccg cccggctcat tcccagcgtt tcacccaact gctcgccaga gtgaaattca
     3181 ccgttcgcta acagggcgat caatttcagt ggcacggtgt tatccttcat gaaattgtct
     3241 ccactgcgct cacttcacct gatgcaccaa taaagcggac ttcaggctca agccagacat
     3301 taaatttttc acccactttt tgtcttacat gatgcgccag ctgtacaaca tcttcgcttt
     3361 ttgcatcgtg ctcatttatg agaaccagcg cctgttgacg gtgtaccgca gccccaccaa
     3421 tttgcgtccc tttaagctgg cactgatcaa tcagccaacc cgcggccagt tttactgaac
     3481 catccacctg ggggtaatgt ggggctgttg gaaattgcgc caacaacgcg ttagcagttt
     3541 cggcagaaac aacaggattt ttgaagaaac tacccgcatt accgttcact tttggatcag
     3601 ggagtttggt ggtgcgcata tgacacaccg catcaaatac ttgctgtggc gttactgttg
     3661 taggatccag acgagttaag tcaccatacg ttagtacagg ttgccactct tttggcagac
     3721 gcagacctac ggctacaatg gcgaagcgat cctggtattc atgtttaaaa atactgtcgc
     3781 gatagccaaa acggcactct ttggcagtta agcgtacttg cttgcctgtc gccagttcaa
     3841 cacaatcaac ataagcgcaa actcgctgta attctacgcc ataagcacca atattctgga
     3901 taggtgatga gccgacacaa ccaggaatta atgccagatt ttccagacca ggcatacctt
     3961 cctgcaaagt gtattttacc aggcgatgcc agttttctcc ggctcctaca tgtaaatacc
     4021 acgcatcagg ttcatcatga atttcgatac ctttgatccg gttgatgatc accgtgccgc
     4081 gatagtcctc cagaaaaagt acattacttc cttcacccag aataagaacg ggttgtcctt
     4141 ctgcggttgc atgctgccag gcattgagta attgttgttc gtcttcggca catacaatgt
     4201 gctgagcatt atgatcaatg ccaaatgtgt tccagggttt taaggagtgg ttcatagctg
     4261 ctttcctgat gcaaaaacga ggctagttta ccgtatctgt ggggggatgg cttgtagata
     4321 tgatgacagg aagagtttat agaaacgcaa aaaggcccag tcttccgact gggctctt
//
LOCUS       DAZFFE010000199         4251 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZFFE010000199 DAZFFE010000000
VERSION     DAZFFE010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.201"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            40..558
                     /locus_tag="ACHJ69_004727"
     CDS             40..558
                     /locus_tag="ACHJ69_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO5017523.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNAQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            574..1113
                     /locus_tag="ACHJ69_004728"
     CDS             574..1113
                     /locus_tag="ACHJ69_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO5017524.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1333..1815)
                     /locus_tag="ACHJ69_004729"
     CDS             complement(1333..1815)
                     /locus_tag="ACHJ69_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HFO5017525.1"
                     /translation="MIWAFAKAYWKQLVIMAMLAVLVISGVVAWNAHGSRQYDAGYAQ
                     AKKDRKAEDEIVRQHYEQEKSINEREAQQRIDQARNDALDAAARAGRLQQQLVAIREQ
                     LRHYNAIVGAGSSAADTGVLLSDVFSKSLERNRQLAEYADRASEAGRVCEKQYDTLTR
                     "
     gene            complement(1812..2441)
                     /locus_tag="ACHJ69_004730"
     CDS             complement(1812..2441)
                     /locus_tag="ACHJ69_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HFO5017526.1"
                     /translation="MEIKQFQRAAGISEALAARWFLHITAAMNEFGIIKPDDQAMFIA
                     QVGHESGGFTRLQENFNYSVNGLSGFIRAGRITPDQASTLGRKAYEKSLPLERQRAIA
                     NLVYSKRMGNNAPGDGWNYRGRGLIQITGLNNYRDCGNGLKVDLLESPELLAQDEYAA
                     RSAAWFFASKGCMKYTGDIARVTLIINGGRNGIDDRRARYITASKVLAV"
     gene            complement(2431..2739)
                     /locus_tag="ACHJ69_004731"
     CDS             complement(2431..2739)
                     /locus_tag="ACHJ69_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO5017527.1"
                     /translation="MATYELFFLITNAVICTGIAIRVVTFRRNGSQHRRWGGWLAYFL
                     IVAAASIPVRVAYAIWLRTPMAVDLSEVIINAVMLAAVIKTRGNVVQIFKVSRSKHGD
                     "
     gene            complement(2726..3130)
                     /locus_tag="ACHJ69_004732"
     CDS             complement(2726..3130)
                     /locus_tag="ACHJ69_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin"
                     /protein_id="HFO5017528.1"
                     /translation="MSVQLTSETLNQWLSLGSLAAVIAGVPPEVALGSLSGAVIFVTS
                     AVEYPISRRVLLSMLSFLCGLLFYKPAASILIGVASLIPTITQDSFEKGVVFSAGAFV
                     SAIVAVRIGIWLYHRSDNPRELIPGRKDDGNV"
     gene            3546..>4251
                     /locus_tag="ACHJ69_004733"
     CDS             3546..>4251
                     /locus_tag="ACHJ69_004733"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO5017529.1"
                     /translation="MLVKDALKRDNNNLDLIRIFCAISVIFAHSYYLANSDGAQEPVQ
                     SVFTFTYTGSIAVKIFFFISGMLVTNSLLTTRNIPHFISSRFFRIFPAFIFVLLFATF
                     IVGPLLTTHSMTSYLKDTLTYSYLFKSSYLDMQFFLPGVFEENIYKSSVNGSLWTIPY
                     EVASYVVLLACYMITQSRGRIIPSIICLSIIALPVLGWNKLIFINNDNADVFLMAPCF
                     ALGALYAINKDKIYISL"
BASE COUNT         1208 a          991 c          944 g         1108 t
ORIGIN      
        1 taaatgtctc atttttcgta atgtgtataa caaggaatag tgatgaaatt taaaaaatgt
       61 cttctgcctg tggcaatgtt agcgtcattc actctggcag gatgccagtc aaatgctgat
      121 gatcatgccg ccgatgttta tcaaaccgat caactgaata ccaaacaaga aactaaaacc
      181 gttaatatta tttccattct tcccgcaaaa gttgccgtag acaacgccca aaataaacgg
      241 aacgcacaag ccttcggcgc gcttattggc gctgtcgctg gcggtgttat cggccacaac
      301 gtcgggtctg gcagcaattc cggaacgacg gcaggtgcag ttggcggcgg agctgtaggc
      361 gcggcagcgg gttcgatggt gaatgataaa accttagtgg aaggtgtttc tttaacatat
      421 aaggaaggca ccaaagtgta tacctccacc caggtgggta aagagtgcca gtttacgaca
      481 ggtttagccg ttgttattac cacgacgtat aacgaaacgc gtattcagcc aaataccaaa
      541 tgtcctgaaa agagctaata atcaggagga gtcatgaaga aagtttttct ttgcgccatc
      601 ttagcctcct taagctatcc ggctatcgcc tcatcattgc aggatcaact ctcggctgtc
      661 gcagaagcgg aacagcaagg taaaaatgaa gagcaaaggc agcatgacga atgggtcgcg
      721 gagcgcaaca gggaaatcca gcaagagaag caacgtcgcg caaacgccca ggccgcggct
      781 aataaaagag cggcaacggc agcagcgaat aagaaagctc gtcaggataa actggacgcc
      841 gaagccactg cggacaaaaa acgcgatcaa agttatgaag atgagctacg tagcttagag
      901 attcagaaac aaaaactggc gctggcgaaa gaagaagccc gcgtcaagcg cgaaaacgaa
      961 tttatcgatc aggaactgaa gcacaaagct gcgcaaaccg atgtggtgca atctgaagct
     1021 gacgcaaaca gaaatatgac tgaaggcggt cgcgatctga tgaaaagcgt gggcaaagca
     1081 gaagagaaca aatcggacag ctggtttaat taatcgatgt aagtaacttc aagcctataa
     1141 ttcttgaaga taaaaaaccc tctgtagtaa cagagggttt tgttcattca tagtgcaggg
     1201 atcaaaatca ttcccactca attatttacg ataaccataa ccaattgagt gataacattt
     1261 ttccaaatct caatttttcc cgtaccgttt tatataccgt caccggaaat cagtaccgtg
     1321 aaaaatgcca tgctatctgg tcagggtgtc gtactgtttt tcgcagactc ttccggcttc
     1381 ggatgcccgg tcagcatact ctgccagttg tctatttctc tcgagagatt tgctgaacac
     1441 gtcggaaagc aaaactccgg tgtctgcggc tgacgaccca gcgccgacaa tggcgttata
     1501 gtgcctgagc tgctcacgga tggcaacgag ctgttgctgc aaccggccag cgcgagcggc
     1561 agcatcaaga gcatcattgc gcgcctggtc gatcctctgc tgcgcttcac gttcattgat
     1621 cgatttctcc tgttcgtagt gctgacgaac tatctcatct tcagctttgc ggtctttctt
     1681 cgcctgcgca tacccggcat cgtactgacg actgccgtgt gcattccagg ctacaactcc
     1741 tgatatgacc agaacagcaa gcatcgccat gataaccaac tgtttccagt atgcttttgc
     1801 gaatgcccag atcataccgc cagcacctta ctggcagtga tgtaccgcgc gcgccggtcg
     1861 tcgatgccgt tccggccacc attgataatc agagttacac gtgcaatatc gccggtatac
     1921 ttcatgcatc ctttgctggc gaagaaccac gctgcgctac gagccgcata ttcgtcctgc
     1981 gccagcagtt cagggctctc cagcaggtca accttcaggc cgtttccgca atcacgatag
     2041 ttattcaaac cggtaatctg gataagtccg cgccctcggt aattccagcc atcaccaggg
     2101 gcattgttcc ccatgcgttt gctgtacacc agattggcga tcgcacgctg gcgctccaga
     2161 ggcagagact tctcatatgc ttttcgtccc agcgtgctgg cctggtccgg cgtgatgcgt
     2221 ccggcacgga taaacccgga cagtccatta acgctgtagt tgaagttttc ctgtaaccgg
     2281 gtaaatcctc cggactcatg cccgacctgc gcgataaaca tcgcctggtc atctggtttg
     2341 ataatgccaa actcattcat ggcagcagtg atatgcagaa accagcgtgc ggccagcgcc
     2401 tcgctaatac cagcagctcg ctggaattgt ttaatctcca tgtttagacc tcgatacttt
     2461 gaaaatttga acgacgttac cgcgcgtttt aataaccgca gcgagcatga cagcgttgat
     2521 aatgacctca gataaatcca cagccattgg cgtgcgtaac cagattgcat aggcgacacg
     2581 aacaggaata ctggccgcag caacaatcag gaaataagca agccaccctc cccatcttcg
     2641 gtgttgtgac ccgttacgcc gaaatgtgac aacgcgaatt gctatgccag tacaaataac
     2701 tgcattggtg ataagaaaaa aaagctcata cgttgccatc gtcttttctc cccggaatta
     2761 actcacgtgg attatcggaa cggtgataga gccagatgcc aattcgtact gcgacgattg
     2821 ctgacacgaa tgcgccagca gagaaaacaa cccctttctc gaaagagtcc tgcgtgatag
     2881 tagggatcag gctagctacg cctataagaa ttgatgctgc tggtttgtag aaaagaagcc
     2941 cacagagaaa gctaagcatc gacaagagta cgcgacgact aattgggtac tctaccgcag
     3001 aggtaacaaa aatcaccgct cctgacaaag accccaacgc tacttctgga ggtactcctg
     3061 ctatcaccgc agctagagaa ccaagactaa gccattgatt taatgtttca ctggttagtt
     3121 gaactgacat agaaaccacc gtttactgtg cataaagacc caccttagtt ggtgagtcca
     3181 tcatacacaa ttaatacata tatggtttaa ttgaaatccc caaaaacatt ctgaataggt
     3241 gaggtgtttt ctggctccat agctatgacg tggcttttga tgcctaactt tataaggtgc
     3301 cacgacaatg tgataggtac ataggattca gttaccaatg caaagctttg tgtgtctcag
     3361 ttttgtctca tgccaaccca gtagaatcaa tctacttaat ctctctgcag tgaatatttt
     3421 gtttgcacgc aattgcacat gatgaactgg cgcttatgat aatttctgac ggtgatctga
     3481 tgggagtgaa agtgcggcta tctgatgata caatgctctt caaaataaac agttaggtga
     3541 tttttatgtt ggtcaaagat gcgctaaaaa gggataacaa taaccttgac ttaatacgta
     3601 ttttttgtgc aatttctgta atatttgccc attcatatta tcttgcaaat tctgatggag
     3661 cccaggagcc ggttcagtct gttttcacat ttacatatac tggttccatt gctgtaaaaa
     3721 tatttttctt tattagcgga atgcttgtaa ctaatagttt attaacaacg cgaaacatac
     3781 cacactttat ttcatctaga ttttttagaa tattccccgc attcattttt gttttattat
     3841 tcgcgacatt cattgttgga ccattactta caacgcattc aatgacatct tatttaaaag
     3901 atacgctgac gtacagctat ctatttaaaa gctcatattt ggatatgcaa ttttttcttc
     3961 ctggtgtttt tgaggaaaat atatataaaa gctcagttaa tggttcttta tggacaatac
     4021 catatgaagt tgcatcttat gtcgtgctcc tagcatgcta catgataact cagagcagag
     4081 gaagaataat acctagcatc atatgcttat caataatagc gcttccagta ttgggatgga
     4141 acaaattaat ttttataaat aacgataatg cagatgtgtt tttaatggct ccatgctttg
     4201 ctcttggcgc attgtatgct ataaacaagg acaaaatata catttcacta c
//
LOCUS       DAZFFE010000200         4238 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZFFE010000200 DAZFFE010000000
VERSION     DAZFFE010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.202"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            182..472
                     /locus_tag="ACHJ69_004734"
     CDS             182..472
                     /locus_tag="ACHJ69_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO5017530.1"
                     /translation="MSTAASVRKIPRENQINIRATDEERAVIDYAASLVNKNRTDFII
                     ELAYQEAKNIILDQRLFVLDNEGYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            463..945
                     /locus_tag="ACHJ69_004735"
     CDS             463..945
                     /locus_tag="ACHJ69_004735"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5017531.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQILNAS
                     RTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRIANLAEQVGIKAVMVHAIDDDARVFYEHFGFVQSVVAPDTLFYKI
                     "
     gene            1230..1487
                     /locus_tag="ACHJ69_004736"
     CDS             1230..1487
                     /locus_tag="ACHJ69_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO5017532.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGKPLSEAERQRAASARKRSVCKEI
                     KVFVRPELKNYLTSLCAEEGVTQAELIERLIEKEACHRNMM"
     gene            complement(1523..1756)
                     /locus_tag="ACHJ69_004737"
     CDS             complement(1523..1756)
                     /locus_tag="ACHJ69_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001410080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HFO5017533.1"
                     /translation="MSTERSLELFRAYNYTPRIEQQLLCGFLLAPVIFSVAKLAEDYR
                     GFHFSGSCKSTSEPVPCHLTAWPATKFLKRSVI"
     gene            1747..1794
                     /locus_tag="ACHJ69_004738"
     CDS             1747..1794
                     /locus_tag="ACHJ69_004738"
                     /inference="COORDINATES: protein motif:HMM:TIGR03475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017534.1"
                     /translation="MCSLLLCNISAGRCD"
     gene            1787..2644
                     /gene="repA"
                     /locus_tag="ACHJ69_004739"
     CDS             1787..2644
                     /gene="repA"
                     /locus_tag="ACHJ69_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO5017535.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSVLFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDAGRERQDIVTLVKRQLTREIAEGRFTANGEAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3611..3862
                     /locus_tag="ACHJ69_004740"
     CDS             3611..3862
                     /locus_tag="ACHJ69_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO5017536.1"
                     /translation="MPNIILSDTSASVSELKKNPMATVSSGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSNQPLHDADLDSYL"
     gene            3859..4146
                     /locus_tag="ACHJ69_004741"
     CDS             3859..4146
                     /locus_tag="ACHJ69_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5017537.1"
                     /translation="MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRECSNVYNLASERMR"
BASE COUNT         1116 a          983 c         1043 g         1096 t
ORIGIN      
        1 cacattaaac ggtcgtaatc agacatgatt tgtgccccaa cacagatcat tgtcacaatt
       61 ttcaagtctc tgtcattgtt tttatcaggg attatttttc ctgcaagtga tttcgtatag
      121 cgttctgtta ccatttgcga taacattcat tgtatagcaa atcgttacca atggaggttt
      181 tatgtccact gcagcaagcg tccgtaaaat accgcgagaa aatcagatta acatccgggc
      241 aactgatgag gaaagagccg ttattgatta tgctgcgagt ctggtcaata agaatcgtac
      301 cgatttcatc attgaactgg cttaccagga agctaaaaac atcattctgg atcaacgttt
      361 atttgtattg gataacgaag gttatgattc gtttattacg caactggaag ctcctgtaca
      421 gaatgcagaa gggcgtgaac gtctgatggc ggtaaagcct gaatggaaat aaatgtcacc
      481 gcgccggcat tgctgacaga tgagcatata cttcagccat ttgactgtgg gaatgaggtg
      541 ttaagtaact ggttgcgtgg tcgggctatg aaaaaccaga tactcaatgc ttctcgcacg
      601 ttcgtcattt gtctggagga tactttacgt gttgtggggt attactcatt agctactggt
      661 tctgtcacac atgccgagct tgggcgcagt ttgcgccata atatgcccaa tcctgtacct
      721 gttgttcttt tagggcgtct ggctgtcgat gtttgtactc agggacatgg tttcggtaaa
      781 tggcttctga gtgatgccat tcatcggatt gccaatctgg ctgaacaggt tggcattaag
      841 gctgttatgg ttcatgcaat tgatgatgat gccagagttt tttatgagca ttttggtttt
      901 gttcagtcag ttgtggcacc tgataccttg ttttataaaa tttgatgtta cgcaatttta
      961 ttgggcgtag ctttataatg atgtaaatcg atagtccctc ggactagctt tcacatttca
     1021 gaaatcgccg atcccgcccg gggccggcac acacgtttag ttattttctg ttttttcgtc
     1081 atttcattat caaaatcaca ttaaacggtc gtaatcaggc atgatttgtg cgccaacaca
     1141 gatcatcgtc acaactttca agtcgctgat ttaaaaaaac tgtagtatcc tctgcgaaac
     1201 gatccctgtt tgagaattga ggaggcgaga tgtcgcagac agaaaatgca gtgacttcct
     1261 catctggcgc aaaacgagca tacagaaagg gtaaaccttt gagtgaagca gagcggcaga
     1321 gggctgcttc tgcgcgcaaa cgatctgtat gtaaagagat taaggttttc gtcaggccag
     1381 aactgaaaaa ctaccttacc agtctgtgtg cagaggaagg ggtaactcag gcagagttga
     1441 tagaaaggct tatcgagaaa gaagcctgtc accggaatat gatgtgatgg gatcacattc
     1501 ttgctagttt tttctgtcaa tgctagatta ctgagcgttt taggaatttt gtggctggcc
     1561 acgccgtaag gtggcaggga actggttctg atgtggattt acaggagcca gaaaagtgaa
     1621 aaccccgata atcttcagca agtttggcga ctgagaagat taccggggct aacaagaaac
     1681 cgcatagaag ctgttgctct atgcggggag tatagttata tgcccggaaa agttcaagac
     1741 ttctttctgt gctcactcct tctgtgcaac ataagtgcag gaaggtgtga ctgatcttca
     1801 acaaacgtat taccgccagg taaagaaccc gaatccggta tttacacccc gtgaaggtgc
     1861 cggaacgctg aagttctgcg aaaaactgat ggaaaaggcg gtgggcttca cctcccgttt
     1921 tgatttcgcc attcatgtgg cgcatgcccg ttcgcgtggt ctgcgtcggc gcatgccgcc
     1981 ggtactgcgt cgacgggcta ttgatgcgct cctgcagggg ctgtgtttcc actatgaccc
     2041 gctggccaac cgcgtccagt gctccatcac cacactggcc attgagtgcg gactggcgac
     2101 ggagtctgct gccggaaaac tctccatcac ccgtgccacc cgggctctga cgttcctgtc
     2161 agagctggga ctgattacct accagacgga atatgacccg cttatcgggt gctacattcc
     2221 gaccgatatc acgttcacat ctgtactgtt tgctgccctc gatgtatcag aggaggcagt
     2281 ggccgccgcg cgccgcagcc gtgtggaatg ggaaaacaga caacgtaaaa agcaggggct
     2341 ggataccctg ggtatggatg aactgatagc gaaagcctgg cgttttgtcc gtgagcgttt
     2401 ccgcagttac cagacagagc ttaagtcccg gggaataaag cgtgcccgtg cgcgtcgtga
     2461 tgcgggcagg gaacgtcagg atatcgtcac cctggtgaaa cggcagctga cgcgtgaaat
     2521 cgcggaaggg cgcttcactg ctaatggtga ggcggtaaaa cgcgaagtgg agcgtcgtgt
     2581 gaaggagcgc atgattctgt cacgtaaccg caattacagt cggctggcca cagcttcccc
     2641 ctgaaagtga cctcctcaga ataatccggc ccgcgccgga ggcatccgca cgcctgaagt
     2701 ccgtcagcgc acaaaaaatc agcaccacat acaaaaaaca acctcaccat ccaccttctg
     2761 gagcatccgg ttccccctgt ttttaataca aaacacgcct cacagacggg gaattttgct
     2821 tatccacatt taactgcaag ggacttcccc ataaggttac aaccgttcat gtcataaagc
     2881 gccagccgcc agtcttacag ggtgcaatgt atcttttaaa cacctgttta tatctccttt
     2941 aaactactta attacattca tttaaaaaga aaacttattc agtgcctgtc ctgtggacag
     3001 acagatatgc acctcccacc gcaagcggcg ggccccgacc ggagccactt tagttacaac
     3061 atgcaagcat agacctcgga aaaacccaag accaccgcag aactgaagcc gcaaagcccc
     3121 tccctcataa ctgaaaagcg gccccgcccc ggcccttcgg gccggaacag agtcgctttt
     3181 aattatgaat gttgtaacta tactccgtta tcgctgtcag tcttctggct ggatgttttg
     3241 agtacacgct cgtaagcggc cctcacggcc cgctaacgcg gagatacgcc ccgacttcgg
     3301 gtaaaccctc gtcgggacca ctccgaccgc gcacagaagc tctctcatgg ctgaaaacgg
     3361 gtatggtcta gcagggcaag ggatgggtaa ggtgaaatct atcaatcagt accggctgac
     3421 gccgagcttc ggcggtttta ctcctgcgcc atatgctaca acggagttcc gccttccatg
     3481 ccgctgacgc ggcatcagtg caatacaagc aacattttgt gtattttcaa aagcacgagt
     3541 aataaagact ttttaagtgg tcatttcaga ggtatcttta agacttgtta aacagtctca
     3601 aaggtacgtt atgcctaaca tcatcctgtc tgacaccagt gcaagtgtca gtgaactgaa
     3661 aaaaaatcct atggctacag tcagttccgg agatggttac cctgtcgcca ttcttaaccg
     3721 gaatcagccg gcattctact gtgtgcctgc tgaactgtat gaaaggatgc ttgatgctct
     3781 ggatgaccag gaactggtga aactggttac tgaacgcagc aatcagccac tgcatgatgc
     3841 ggacctggac agttatttat gacttatacg gtaaaattca gggatgatgc gctcaaagaa
     3901 tggctgaaac tggataagac cattcaacaa cagtttgtga aaaaactaaa aaaatgcagc
     3961 gaaaatcctc atataccgtc ggcaaaacta cgtgggctaa aggactgcta caaaattaaa
     4021 cttcgtgcat cgggatttcg tcttgtttat caggtgattg acgatatgtt aattattgcg
     4081 gttgttgcgg taggtaaacg agaatgtagt aacgtttata atctcgccag tgagagaatg
     4141 agataggaac atataaatag cctttcccgc agataactct cagaattgtt atattctctg
     4201 tggactcgcc tgaggcagga aaagaaaagt tttgttga
//
LOCUS       DAZFFE010000201         4105 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZFFE010000201 DAZFFE010000000
VERSION     DAZFFE010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.203"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..146
                     /locus_tag="ACHJ69_004742"
     CDS             <1..146
                     /locus_tag="ACHJ69_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO5017538.1"
                     /translation="YRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQ
                     MAA"
     gene            300..2297
                     /locus_tag="ACHJ69_004743"
     CDS             300..2297
                     /locus_tag="ACHJ69_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFO5017539.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2360..3637
                     /locus_tag="ACHJ69_004744"
     CDS             2360..3637
                     /locus_tag="ACHJ69_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HFO5017540.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKK"
     gene            3883..4044
                     /locus_tag="ACHJ69_004745"
     CDS             3883..4044
                     /locus_tag="ACHJ69_004745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017541.1"
                     /translation="MTGENRGRGRPRKTESTYADTRNDLIRSGLELLTQNGFLATGVD
                     AIVSTTDEK"
BASE COUNT         1010 a          942 c         1026 g         1127 t
ORIGIN      
        1 actatcgttt taataaccgg gatgaagcca tctcagtaat acgggaatac attgagattt
       61 tctacaatcg tcagcgtcgt cactctcgtc tggggaatat ctccccggca gccttcaggg
      121 aaaaatatca tcagatggct gcttaaaaaa agaacaactg gtagtgtccg ctattgccag
      181 tacacctcaa cattccacca tgcattccga ttaacgccgc atagccagtt gaactttgct
      241 actttgtgag aggtagtacc ttctatccag tgcgaattta attaatggaa taaatgatta
      301 tgagtgaaaa tgatacaatc ccaaagaagt ctacaagtca gattaacaaa gcggtattct
      361 ttacatctgc tttgctaatt ttccttcttg tcgcctttgc cgccgtattc ccggatgtcg
      421 ccgacaaaaa ttttaaacta cttcagcaac aaatcttcac gaatgccagc tggttctaca
      481 tccttgctgt ggccctgatt ttactgagtg tcacgttcct tggactctca cgctacggtg
      541 atatcaagct gggcccggac catgcgcagc ctgatttcag ctaccactcc tggtttgcga
      601 tgcttttttc ggcagggatg gggatcggcc tgatgttctt tggcgttgcc gaacctgtaa
      661 tgcattatct ttcgccaccc gttggcactc cagaaaccgt tgcggcagct aaggaagcaa
      721 tgcgtctgac ctttttccac tggggactgc acgcatgggc aatttatgcc attgtggcgc
      781 tgattctggc gttcttcagt taccgtcacg gtctgccttt aactctgcgc tccgcactct
      841 atcccattat tggcgatcgc atatacggac ctgtaggaca tgcggttgat attttcgctg
      901 ttataggcac ggtctttggc gttgcgacat cactgggtta cggtgttttg caggtgaatg
      961 ccggtttgaa ccatcttttc ggggtgccca tcaatgaaac ggtgcaggta attctgatcg
     1021 tggtcatcac ggggttagcg acgatttcag tggtgtccgg tctggataag ggaatacgta
     1081 tcctgtctga actcaatctg ggtctggctt tgttgctcct ggcgctggtc ctgtgtctgg
     1141 gaccaaccgt gcttctgctg aagtcatttg tggaaaatac gggcggttat ctttcggaac
     1201 tggtgagtaa aacgttcaac ctttacgcgt atgagcccaa gtcgagcaac tggctggggg
     1261 gctggacatt actgtactgg ggatggtggc tttcatggtc gccgtttgtg gggatgttca
     1321 tcgcacgggt ctcccgcggg cgaaccattc gcgagtttgt caccggcgtg ctgtttgttc
     1381 ccgcgggttt tacgctaatg tggatgacgg tgtttggtaa cagcgcgatc tatctcatta
     1441 tgaaccaggg ggccacagac ctcgccaata ctgttcagca ggatgtggcg ctggccctgt
     1501 ttaatttcct ggagcatttc ccgttctctt ctgtgctgtc attcattgca atggcgatgg
     1561 tcatcgtctt ctttgtaacg tctgctgatt cgggggcaat ggttgtggat actctggcat
     1621 caggtggagt ggcaaacaca cccgtctggc agcgaatatt ctgggcctcg ctcatgggca
     1681 ttgttgcaat tgcgcttctc cttgccggag ggctaagtgc gctgcaaacg gtgacaatag
     1741 cgagtgcatt gcccttctca gtgatcttac taatatctat atacggactt ttaaaagctt
     1801 tgcgccggga tttgaccaag cgtgaaagcc tgagcatggc gacaattgct cctacggctg
     1861 cacgtaaccc aattccttgg cagagaaggt tacgcaatat cgcgtatctg ccgaagcgat
     1921 ctcttgtgaa acgttttatg gacgacgtta tccagcccgc catgacgctg gttcaggagg
     1981 aactgaacaa gcaggggacg ataagccaca ttagtgatgc agtcgacgat cgtattcgtc
     2041 ttgaagtcga tttgggcaac gagctgaatt ttatatatga agtgaggctt cgcgggtata
     2101 tctcaccgac cttcgcgctc gccgcaatgg ataatgatga gcagcagact gaacaacatc
     2161 gatattatcg cgctgaggtt tatctcaaag aaggcggtca aaattatgat gtgatgggct
     2221 ggaaccagga acagctgatt aatgacatac tggaccagta cgaaaaacac ctgcacttcc
     2281 tgcacctggt tcgttaatag caacatgccg tcctgggggc ggcaattatt atcccggccg
     2341 caatatgagg gaatgcagaa tgatttcacg ctggaaatgg atgctgaagc agacaattaa
     2401 aaaactatgg ttcagggcaa cgttattcgc aattgtcgcg ataataacgg cccttttatc
     2461 aattcttttt aaatcaatga tacctgagtc ggtttccgtg aaggttggtg cggaagcagt
     2521 cgataacatt ctgaacatac tggcatcgag tatgctggca gtgaccacat tttcgctgag
     2581 tatcatggtc acagcctacg gttcagccac tactaatgtg actcccagag ctacgcgttt
     2641 agttgttgaa gacgtcacca cacaaaatgt actggccacc ttcatcggtt cttttctctt
     2701 cagtctggta gggattattg ccctcaatat gggagcttat ggagaaaggg ggagagtcat
     2761 tttattcatt gtcacactgg ttgtcattgc cttaatcctc atcacattgc ttcgctggat
     2821 acagcatttg acctctctgg ggagggttgg tgagacaacg gcacaagtag aacaggcggc
     2881 catcgaaaca tttattgcga gagcaagaaa tccctgtctc ggcggatatc catggcttga
     2941 gaacaatgaa cagccgaaag gaacggttgc agtttatccg aagaagattg gctatgttga
     3001 atatattgat atggtgaaac ttagcaagct gctgaccaat gatccccgtc atgtatacct
     3061 cgtggcgcag ccaggcagtt tcatacatcc gtccatgcca gttttgtacc tgagtcaggg
     3121 ccaggagtcg tcaatcaaca ccgatttact tgagacgatt attgtctcgg atgcacgttc
     3181 atttgctcag gatcctcgat tttgtcttag cgtcatggcc gaaatagcct gccgggccct
     3241 ttcccctgca gtgaacgatc ctggaaccgc cattgatgtc attggcagag gtgttcgtat
     3301 actttccact tacgcgcaga ataaatctga tgaaatagaa gtgaaatatc cttcagtaca
     3361 tgttgcacca cttcagaata acgatctact ggaagacttt ttctcacctg tcgcgcgcga
     3421 tggtgccagt atgagggaga ttcagataag agtccttaaa ggactgtcga tgctgagtaa
     3481 aggttggcct gagatatttg ctgaagccgc acataccctg gcatttgaaa cattagagca
     3541 tgcaactcgt gctgaccata tagattctga tagatatcta ataaaatcaa cttattataa
     3601 tttatttagt ggcgaatatt ctaataaaaa aaaatagctg cagagcaagc aattcggttg
     3661 ttggcaagtc aagggcaaag cattcgcgtg agaatgggcg tatgctttgt gccaaaagca
     3721 aacgttgcta attctgcact gttcagcgat ggcgcagtag cgacaggcac tgccactgtc
     3781 cagtcggcaa aagcgaacac tttgccactg cggggataaa ctgattgaag ctctcgcgca
     3841 aaatcactta ttcttttgac aataaaatgt aggagatggg tcatgacagg cgaaaatcgt
     3901 ggccggggaa ggccgcgaaa gacagaaagt acctatgcag atactcgtaa tgatctgatc
     3961 cgcagtggcc ttgaactgct gacgcagaat ggttttcttg cgacgggagt cgatgccatt
     4021 gtgtcaacga cggatgaaaa gtgatccact tatatctcca ccaacggccc aatattgatc
     4081 caccgtttta ctcaggatta gcttc
//
LOCUS       DAZFFE010000202         4011 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZFFE010000202 DAZFFE010000000
VERSION     DAZFFE010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.204"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            47..367
                     /locus_tag="ACHJ69_004746"
                     /pseudo
     CDS             47..367
                     /locus_tag="ACHJ69_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            608..1573
                     /gene="lipA"
                     /locus_tag="ACHJ69_004747"
     CDS             608..1573
                     /gene="lipA"
                     /locus_tag="ACHJ69_004747"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO5017542.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(1673..1876)
                     /gene="tatE"
                     /locus_tag="ACHJ69_004748"
     CDS             complement(1673..1876)
                     /gene="tatE"
                     /locus_tag="ACHJ69_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO5017543.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2005..2793)
                     /locus_tag="ACHJ69_004749"
     CDS             complement(2005..2793)
                     /locus_tag="ACHJ69_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO5017544.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENEVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLDRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            2886..3269
                     /gene="crcB"
                     /locus_tag="ACHJ69_004750"
     CDS             2886..3269
                     /gene="crcB"
                     /locus_tag="ACHJ69_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO5017545.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTTH"
     gene            complement(3323..3532)
                     /gene="cspE"
                     /locus_tag="ACHJ69_004751"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3323..3532)
                     /gene="cspE"
                     /locus_tag="ACHJ69_004751"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO5017546.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(3707..>4011)
                     /gene="pagP"
                     /locus_tag="ACHJ69_004752"
     CDS             complement(3707..>4011)
                     /gene="pagP"
                     /locus_tag="ACHJ69_004752"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO5017547.1"
                     /translation="EKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGL
                     GFTAGVTARDNWNYIPLPVLLPLASVGYGPATFQMTYIPGTYNNGNVYFAWMLFQF"
BASE COUNT         1052 a         1020 c          912 g         1027 t
ORIGIN      
        1 atttttttaa atattatttt ttcatgaatg aaattggagt aagtgcgtgg atagtaataa
       61 tcaaattgaa ccctgtttaa gcagaaaatc gtcagaaggt aaaccacaaa tatttacaac
      121 cttacgaaat attgatctta cccttctgac tattttgaag ttgcatatgt ccataaagga
      181 atcgttaatg cagcgaaagt gcttaatctg ccccccctcg gtcggagccc tggtccttcc
      241 tgtcatctat cgggcgatta aaactcacta tccacagctt ttactgcgca acccgcccat
      301 cggcggcgcg gaaaaccaac tcagtcagtt tcaaaccgat ctcatcaccg ataacatgtt
      361 ttgcacagga ccatagatgt tatccgtaat gcattttgaa aaagtattgt tacagccaga
      421 tcacagcgca gaacgataaa atggcacaat ccaacaacaa ttttacattt tcgcgaccgc
      481 tttggctgct ttcaggtccg tttcaatgat atactgccag tcgttaattc aaaaatagtt
      541 gataattaca acaatctatt gaattgaaac gctttccttc ataattcgca actggaacac
      601 gcacgctatg agtaaaccca ttgtgatgga acgcggtgtt aaataccgcg atgccgataa
      661 gatggccctt atcccggtta aaaacgtggc aacagagcgc gaagccctgc tgcgcaagcc
      721 ggaatggatg aaaatcaagc ttccagcgga ctctacacgt atccagggca tcaaagccgc
      781 aatgcgcaaa aatggcctgc attctgtctg cgaggaagcc tcctgcccta acctggcgga
      841 atgctttaac cacggcacag caacgtttat gatcctcggc gctatttgta cccgccgttg
      901 cccgttctgt gacgttgccc acggtcgccc ggtagctcct gatgccaatg aaccagtgaa
      961 actggcgcag accattgccg atatggcgct gcgttatgtg gttatcacct ccgttgaccg
     1021 tgatgaccta cgcgatggcg gtgcccagca ctttgcggat tgcattactg ccattcggga
     1081 aaaaagcccg caaatcaaaa ttgaaactct ggtgccggat ttccgcggtc gtatggatcg
     1141 tgctctggat attctgactg caacgccacc agatgtgttc aaccataacc tggaaaacgt
     1201 accgcgtatt taccgtcagg tacggcctgg cgcagattac aactggtcgc ttaagctgct
     1261 ggaacgcttt aaagaagcgc atccggaaat cccgaccaag tctggtctga tggtgggact
     1321 gggtgaaacc aatgaagaaa ttattgaggt aatgcgcgac ctgcgccgtc atggtgtgac
     1381 gatgctaacg ctggggcaat atttgcagcc aagccgccat cacctgccgg ttcaacgtta
     1441 cgttagcccg gatgagttcg acgaaatgaa agccgaagcg ctggcgatgg gctttaccca
     1501 tgctgcatgc ggtccgtttg tccgctcttc ttaccacgcc gatttgcagg cgaaagggat
     1561 ggaagttaag taatcctgta ataattactt acatctgtct gataaaaaac ccgctttttg
     1621 aagcgggttt ttgtatcgaa caagatattg agggagcgtc ctgctcgcca cgtcactctt
     1681 tatgagagag cttttcagcc tgaagatcaa cgtctgcgcc ttttttcgcc gcagcatcgt
     1741 catcattcat cgccttcttg aaccctttaa tggccgctcc aaggtctccg cccagcgtac
     1801 gtaacttctt agtcccaaac agcagaacga ccagcgccgc aactaccagc agtttggtaa
     1861 tactaatctc acccatagat accttcttga catataaccg gcaacagaac gcgccttaaa
     1921 aaccagagaa tattaacggt cattcgacgc gcgcacacaa tagcaatctg taacaaggtg
     1981 aatcaagcgc cgagtaaaaa aacatcataa taattgcggc ggcgcaaagc gacggttgtt
     2041 taagacgggc agttgcgcgc gtacctgacg cacacgttcg ggcgtcactt ccgccataat
     2101 gagtgcaggc atttctgacg ctgccgcaat ggtgacgcca aagggatcta taatccggct
     2161 ttgcccgata tttttgttcc cgcactcccc cgccgccacc atataacagg tggtatccag
     2221 cgcacgagcg gcaagcaacg ttgaccagtg atgctctttg agcggcccac gaacccaggc
     2281 ggcaggaagt accaggattt cagctccctg taatgcctgt gccagcgcca gctctggaaa
     2341 gcgtaagtca taacaggtca tcagaccgac cttcatccct tccacctcca gtaacggagc
     2401 tatttcatta ccagcatcaa catggcgtga ttcctgaatg gcaaatgcat catagagatg
     2461 cagtttggca taacgggcga cgatgtttcc tgcctgtagc gccaccagca tattccacgc
     2521 gcgccccggt gttgaaggaa catgaatcgt cagaattgtc gtcatcatgt tacgtttact
     2581 ttctcgccgt aaacggtcga ggaattcgcc ttccagcagc tgtgctgatt taaccgaaag
     2641 atccgcatca tgatcatcac gcgccagtaa tgcttccggc aggacaaaca gcgaaacctc
     2701 gttttccgcc gcctgcgcca tcaacgaggc acaaatctca gcgttctttt cccacacaga
     2761 tgtaacagca aactgtcctg cggcaaccag cattgccacc tcctgtcaga gtcgtaaaat
     2821 tcgccaacgg cgattattcg gcgttacact tatcactcat acaaatcaaa tagcaggatt
     2881 ttgcagtgtt acaacttctt ttagcagttt ttattggcgg tggtacggga agcgtggcga
     2941 gatggctgtt aagtatgcga tttaacccgc tgcatcaggc gattccgttg gggacgctgg
     3001 cagcaaacct gattggggca ttcatcatag gaatgggatt cgcctggttc agcaggatga
     3061 cgaacattga tccagtgtgg aaagtattaa tcaccaccgg attttgtggc ggtctaacaa
     3121 ccttctcaac attttcggca gaagtggtgt ttttgttaca agagggccgc tttggctggg
     3181 cgttactgaa cgttttcgtc aatcttttgg ggtcttttgc catgaccgca ctggctttct
     3241 ggctgttttc ggcctcaacc acacactaaa gaccaaaaaa aacccgctga ttaagcgggt
     3301 tttgaattct tgctgacgta tcttacagag cgattacgtt tgcagcagaa gggcctttgg
     3361 caccgttagt gatttcgaac tctacgcgct gaccttcagc aagagtttta aaaccattag
     3421 tctggattgc agagaagtgt acgaacacgt ctttgctgcc gtcttccgga gtaatgaaac
     3481 cgaatccttt ggactcatta aaccacttaa cgttaccttt aatcttagac atcaaaatta
     3541 cctttacatg aaaaatagac acaaatgctg tgtcggttac cagtacacca attgtggtac
     3601 gctttgtcca gtgcaacttt actaaaaagt gataaaagtc gcgttatttt tgatgttaat
     3661 tattttagtg gttgatttta aaggatttac taaaacttca tttgtctcaa aactgaaaga
     3721 gcatccaggc aaagtacaca ttgccattgt tgtaggtacc cggaatgtag gtcatctgaa
     3781 acgtcgcggg gccatacccc acagaggcca atggcagtag aaccggaaga gggatgtaat
     3841 tccagttatc gcgcgccgtt acgccagcgg taaatcccag gcctaaatga aaattttcat
     3901 ccgccagcgg tcgccaggta ctttcccatc cgtatccggc aatcggttcc catttgttcc
     3961 acgagtcctt aaatgccatt gcatacaggc catgccagtt tcctttttca t
//
LOCUS       DAZFFE010000203         3916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFFE010000203 DAZFFE010000000
VERSION     DAZFFE010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.205"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..3434
                     /locus_tag="ACHJ69_004753"
     CDS             <1..3434
                     /locus_tag="ACHJ69_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFO5017548.1"
                     /translation="VRETIRTLGQRVREGYTPREDGAGASSQYSRSGKPDPFKVPEGE
                     GERYRDDLARMMKSLRSGAMTANIGRTPPVLRHLGAPDLPLVISRDTVRKATNGVKHV
                     VPMDVIERLPELMHDPDAIYRSATERNAVVMLLDAVDKNGDPVVSAVHMKATDKRIEI
                     NKVASVYGTKGGMNKANSLDKAGLTLYRKEKLSRDNPQYSGLQLPKEERSYRGSVDKI
                     LYPEDIRKGPYYSRTSSLTPEETIASRFVRQMQDKFQVLKAVQENIRKTGGKIDDSNN
                     AYMAEELFHGKAENDLNVMKERYVQPLAKLLADYKIAQADLDEYLYARHAPERNAHIA
                     KINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQYDRLAGIVDDMLARRRELIREAGLE
                     ESGVVDAWQNAYRYYVPLKGQDIDGVVSLPRTGKGFTIGGRESWQAMGRASRAQSPST
                     QAIQDLSESLIRHRKNEVGNAFLKLVQDNPDKDYWQVFTDDRPDTMRVIAERKDQETG
                     ETIREVVERPVAMAMMADRYFTTKKDGKTYYIKLHDPRLMRAMKNMGPETSNAVIRTL
                     GKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNLKAEQGRSDGKLKELDNLSALAVVK
                     DSRSAMSAVYASLRGKTLTGKGAQWQKVWKEFVEDGGKTGWFNMGDLEGQQKEMDRLV
                     SLAKGGWKGQSIGAWHSFLNLVEDANGAVENALRLSAYKHARDAGLSRQQAASLAKNM
                     TVNFNRRGEQGMLMNSLYMFANASIQGTANLVRTLGHLNGDGPLPERLRWKNLNVPQK
                     IVLAAVGAGYLLGSLNRSVAGEDDDGVNWYDKVPSHVKERNLVIMKSMFGGKAGEYWS
                     IPLPYGYNVFFLLGHTAEGVAAGDLTASRAAGNVVGGILGAFSPIGSETSETLSGALL
                     KNAAPTILRPFANLAMNENFMGAQIYQENMPFGTPKPDSQLGRRSTPEAYKAFASWLN
                     AFSGGSQYRPGAVDITPESLKYWVDYISGGTGRFISKTTDAAVKSLNGIDIPEQQVPF
                     LGKISGEVMPYADQQKMYDRMTEVAQYHAELKSLTGAERTAFIDENNGKLSMNGLMQD
                     TRKRLKDLRKQRDAIYADSTLSLAQQSAMVKSVERDMKIAVDRFNREYNKKVGVE"
     gene            complement(3656..>3916)
                     /locus_tag="ACHJ69_004754"
     CDS             complement(3656..>3916)
                     /locus_tag="ACHJ69_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017549.1"
                     /translation="LGNTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGG
                     TFIFALMSHAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
BASE COUNT         1001 a          905 c         1158 g          852 t
ORIGIN      
        1 ttgttcgcga aactattcgc acgttaggcc agcgtgtgcg ggaaggttac acgccgcgtg
       61 aggatggcgc gggcgcatcg tctcagtact cccgtagtgg taaacctgat ccgttcaaag
      121 tgccggaagg tgagggcgag cgttatcgtg atgaccttgc cagaatgatg aagtcgttac
      181 gttctggtgc aatgactgca aacatcgggc gtacgccgcc ggtattgcgc caccttggcg
      241 caccagattt gccgctggtt atctcccgcg acactgtgcg gaaggcaacc aatggagtga
      301 aacatgtggt gccaatggat gttatcgaga gacttcctga gctgatgcac gatccggatg
      361 caatttaccg ttccgcgaca gaaagaaatg cggttgtgat gctgcttgat gccgtggata
      421 aaaatggtga tccggtggtg tcggcagtgc acatgaaggc aacagataaa cgaatagaaa
      481 ttaacaaggt ggcttctgta tatggaacaa aaggggggat gaacaaagct aatagcctgg
      541 ataaagcagg tttgacgctt taccggaagg aaaaattaag ccgcgataac cctcagtaca
      601 gtgggcttca attgcccaaa gaggagcgtt cttatcgcgg ctctgtagat aaaatactct
      661 atcctgaaga tattcgcaag gggccgtatt actcccgtac cagcagtctg acaccggaag
      721 agacaattgc atcgcgtttt gtgcgtcaga tgcaggataa attccaggtg ctgaaagctg
      781 ttcaggagaa catccgtaaa actggcggaa aaatagacga cagtaacaac gcttatatgg
      841 cggaagaact cttccatggg aaggcggaaa acgacctgaa cgtgatgaag gagcgctacg
      901 ttcagccact ggctaaatta ctggcggact acaaaattgc gcaggccgat ctggatgagt
      961 acctctacgc ccgtcacgcg ccggaacgta acgcgcatat cgcgaaaatc aacccgaaaa
     1021 tgccggacgg cggttcgggg atgaccaacg cggaggcggc ggaaatcatg cagcgtgtgc
     1081 gtaacagtgg aaaacaggca cagtatgacc gtctggcagg gattgttgac gatatgctgg
     1141 cccgtcgccg tgagcttatc cgtgaggccg gacttgaaga gagtggtgtg gtggatgcct
     1201 ggcagaacgc ctaccgttac tacgttcccc tgaaaggtca ggatattgac ggtgtggtgt
     1261 cactgccccg tacaggtaag ggcttcacta tcggcggacg tgaaagctgg caggccatgg
     1321 ggcgtgcatc ccgtgcacag tctccgtcca ctcaggcgat acaggacctg agcgaatcgc
     1381 tgatccgcca tcgcaaaaac gaagtgggta acgccttcct gaaactggta caggataatc
     1441 ccgacaagga ttactggcag gtattcactg atgacagacc ggataccatg cgtgtgattg
     1501 cagagcgcaa ggaccaggaa actggtgaaa ccattcgcga agttgtcgag cgtccggtgg
     1561 cgatggcaat gatggcggat cgctacttca ccaccaaaaa ggacggcaaa acttactaca
     1621 ttaaacttca tgatccgcgc ctgatgcgtg cgatgaagaa catggggccg gaaaccagta
     1681 acgccgtaat ccgtacgctg gggaaagtta accgcttcct ggcaacggtg aacacgtcgt
     1741 ataacccgga atttctggtc agtaacttca tccgtgacgt gcagactgcg gtgatgaacc
     1801 tgaaggctga gcagggaagg agcgacggta aactgaaaga gctggataac ttatccgcgc
     1861 tggctgtggt gaaagacagc cgttccgcca tgtcagccgt atacgccagt ctgcggggta
     1921 aaaccctcac gggcaaaggc gcacagtggc agaaagtgtg gaaagagttt gttgaggacg
     1981 gaggtaaaac cggctggttt aacatgggtg accttgaggg ccagcagaag gaaatggatc
     2041 gccttgtgtc gctggcgaag ggcggatgga aaggccagag tatcggtgca tggcattcgt
     2101 tcctgaacct ggtcgaggat gccaacggcg cggttgaaaa cgctctgcgt ctttctgcct
     2161 ataaacacgc ccgtgatgcc ggtttgtcac gccagcaggc ggcgtctctt gccaaaaaca
     2221 tgacggtgaa ctttaaccgt cgtggtgagc aggggatgct gatgaactcg ctgtacatgt
     2281 ttgccaacgc cagcattcag gggaccgcaa acctggtgag aacgctcgga catcttaatg
     2341 gcgacgggcc gttaccggag cgccttcgct ggaagaatct caatgtaccg cagaaaatcg
     2401 tgcttgcagc tgtgggagcg ggttatctgc ttggctcgct taaccgcagt gtggcgggtg
     2461 aggatgatga cggggttaac tggtacgaca aagtaccgtc tcatgtgaaa gagcgtaacc
     2521 tcgtcatcat gaaatcgatg ttcgggggca aggccggtga gtactggagt attcctctgc
     2581 cttacgggta caacgtgttc ttcctgcttg gtcatacagc tgaaggtgtg gcagcgggcg
     2641 acctgactgc ttcacgtgct gccggtaatg ttgtcggcgg tatcctgggg gcattcagcc
     2701 cgatcggcag cgagacgtcg gaaacgttgt cgggggcatt gctgaaaaac gcagcaccga
     2761 ccattctgcg tccgtttgcg aaccttgcca tgaatgaaaa cttcatgggg gcgcagattt
     2821 accaggagaa catgccgttt ggtacaccaa aacctgacag ccagctggga agacgttcaa
     2881 cgccagaagc gtacaaggcg tttgcatcct ggctgaatgc gttctcaggt ggcagccagt
     2941 accgtcccgg cgcggtggat atcacaccgg aatcgctgaa atactgggtg gactatatct
     3001 ccggagggac agggcgcttc atttccaaaa ccacggatgc ggcggtgaaa tcgctgaatg
     3061 gtattgatat accggagcag caggtgccct tcctggggaa aatttcaggt gaggtgatgc
     3121 cgtatgctga ccagcagaag atgtacgacc ggatgacaga ggttgcgcag tatcacgcag
     3181 agctgaagag tctgaccggt gcagaaagaa cggcgttcat tgacgagaac aacggaaaat
     3241 tgtcgatgaa cgggcttatg caggataccc ggaagagact gaaggatttg cgtaaacagc
     3301 gtgatgccat ttacgccgac agtactctca gtctggcgca acagtcggcg atggtgaaat
     3361 cggttgagcg ggatatgaaa attgccgtgg atcggtttaa ccgcgagtac aacaaaaaag
     3421 tgggagtgga gtaaagaaca tgccccgtac ggaagtgcgg ggcatgctta acgggatgtg
     3481 tacataaatg ggtacggata tgacgattat tttggtgctt tctgtattta aatcaattag
     3541 ttaatacttg tattcattat atccatttaa ctaagaggac aatgcggcac gagtataccc
     3601 gctaatggac tacggagtaa gtacacagcc gctcgattgc ttaaaccctc gccatttatg
     3661 ccgggttttt atcatttttc ttaatgtttt ccgcacgttc tgctttttga cgtgcttctg
     3721 ctttgcgctt gttgctcatg tcgttacgaa tctgtgcatg actcattaac gcaaagataa
     3781 aggtgccgcc gcagatgttc cccgctaaag taggtagtgc aaagggccag atgaaatcgc
     3841 tccagtgcag cgtgccgtta aacaccagat agaggatttc aacagaaccg acaacgatgt
     3901 gggtggtgtt acccag
//
LOCUS       DAZFFE010000204         3911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZFFE010000204 DAZFFE010000000
VERSION     DAZFFE010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.206"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..462)
                     /gene="yehS"
                     /locus_tag="ACHJ69_004755"
     CDS             complement(<1..462)
                     /gene="yehS"
                     /locus_tag="ACHJ69_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO5017550.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVK"
     gene            complement(509..1228)
                     /gene="btsR"
                     /locus_tag="ACHJ69_004756"
                     /gene_synonym="yehT"
     CDS             complement(509..1228)
                     /gene="btsR"
                     /locus_tag="ACHJ69_004756"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO5017551.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1225..2910)
                     /gene="btsS"
                     /locus_tag="ACHJ69_004757"
     CDS             complement(1225..2910)
                     /gene="btsS"
                     /locus_tag="ACHJ69_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO5017552.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3132..3197
                     /locus_tag="ACHJ69_004758"
                     /pseudo
     CDS             3132..3197
                     /locus_tag="ACHJ69_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3425..3673
                     /locus_tag="ACHJ69_004759"
     CDS             3425..3673
                     /locus_tag="ACHJ69_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO5017553.1"
                     /translation="MRVEICIAKEKISKMPKGSVSALQQEMLRRVSKRYDDVDVIVRA
                     ANNDSLTILRAVDKESAHEFVQETLQEAWETADDWFIR"
     gene            complement(3710..>3911)
                     /locus_tag="ACHJ69_004760"
     CDS             complement(3710..>3911)
                     /locus_tag="ACHJ69_004760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017554.1"
                     /translation="NILRVRSDSVLPDLQRLQLIQGQMMNKDKCRMYGRKRYTAGAMC
                     FMLFVWLGGVVTLCAVAAIIMQ"
BASE COUNT         1043 a         1025 c          887 g          956 t
ORIGIN      
        1 cttcacatgc tggcgcgctg ccagtccacg cagaaaataa cgtaaaaatt gatcgccgca
       61 ttcgcggaag tttttatgat ccggtgcacg catcatcgcc gtaatttccg gcatcgaaac
      121 gcggaactgc tgttcggtga ggattgccag aatgtcatcg gttttaagcg aaaacgcgat
      181 gcgtaatttt ttcagcacga tgttgttatt aatgcgacgt tccggctcca gtgccggagc
      241 agactcatcc ttgccgcgtt tttcataaat caggccattg aggaatgaag acaaaacaat
      301 gtccggacaa cgctgaaaac cctcttcgtc ttctttacgt agccagacgg cgatctgttc
      361 cgcggtggct tcgacattac ccagcgccag aatacgcacc aggtcattat tattggcttt
      421 caaaatgtag cgcacgctgc gcagaatatc gttacttagc atgaggcctt caggtgttga
      481 tgaggcaaaa agccatttta gcagtctttt acaggccaat cgcctctttt aagcttttca
      541 gataacggcg gctgaccggc acggttaagc cattacgcaa aatcaactcg gcctggccgt
      601 tatcttccag acggatctcc tgtaaatgcg cgaggttaac cagatactga cgatggcagc
      661 gcagtaatgg tgtacgactt tccagggtac gtaatgtcag ttcggtaaag ccctctttcc
      721 cttcgtgact ggtaacgtag acaccgctca tccgactgct gacaaatgcc acatctttca
      781 tttgcagcaa ataaatccga ctatgccccg tacaagggat aaatttcagc gcctgttgat
      841 tttccggtaa cagcgaaaca tcctgcttgc tgcgctcctg acgcaatcgc gccagcgttt
      901 tctccagtcg cgcttcatca attggcttca gcagataatc aaaggcatgt tcttcaaagg
      961 ctttaatggc gtattcgtca aacgcggtga gaaaaacaat atacgggcga tgttccggat
     1021 caagcatccc caccatttcc agaccactga tgcgcggcat ctggatatcg agaaacagca
     1081 catccgggcg cagtttatgc accgcgccga tcccttctac ggcgtttgaa cactctccaa
     1141 cgatttcaat atcgctctgc tcctgcaaaa atacgcgcag gttctcccgt gctaacggtt
     1201 catcatcgac aattaagact ttaatcatgc ctcgtccctc catggtagtc gtaacgttat
     1261 tcgggtgtaa ctatcaggct cacaggcgac gcttatccca tagtcatcgc caaaccgttc
     1321 acgtaaacgc ttatccacca gattcatccc cagcccactg gcattggtta ccggttgata
     1381 caaaccggca ttgtcttcga tctccagcat caaatgttgc ccctcacgtc gggcgctgat
     1441 tgccactcgc cctgtatcca acagttgtga tgtcccatgt ttaatggcgt tttccactat
     1501 cggttgcagg gtaaacgcgg gcaattgctg ctgggataat tcttgcggaa tagcaatgtt
     1561 gacctgcaac cgcgactgga agcgcgcctt ttcaatttgc agataagcgt tcacatgttc
     1621 aatttcgtcg gcgagagtaa caaactccga aggccgcttt aagtttttgc ggaaaaaagt
     1681 ggaaagatac tgcaccagct ggctggcctg ttcgctgtcg cggcggatca ccgctttaat
     1741 ggtgttaagc gcattaaaca aaaaatgggg attcacctgg gcgtgaagca gtttgatctc
     1801 tgactgggtg agcatcgctt tttgccgctc atattgcccg gcgaggatct gcgccgaaag
     1861 cagttgcgca atcccctcgc ccaacgtgcg gttgattgaa ctgaataaac ggtttttggc
     1921 ttcatacaat ttgatggtgc ccatcacccg ctgattttcg ccacgcaacg gaatgaccag
     1981 cgtcgacccc agtttgcatt gcggatgcaa agagcaacga taaggtactt cgttgccatc
     2041 agcgtagacc acttcaccgg tttcaatcgc ttttaaggta taagtcgaag aaatcggttt
     2101 gccgggtaaa tggtggtcgt caccaattcc ggtaaaggcc agcaatttct ctcgatcggt
     2161 aatcgcgact gcaccaatat ccagctcctg atacagcacc tgtgccactt tcatgctgtt
     2221 cacttcgtta aacccctggc gcaaaatgcc ttccgtcgag gccgccactt tcagcgcagt
     2281 ggcagaaaaa gccgaagtgt atttttcaaa catcgcgcgt ttatcgagca atatacgcat
     2341 aaacaacgcc gcgccgacgg tattggtgac catcattggc gcagcaatat tactcaccag
     2401 acgcaccgca tcttcataag gtcgggcgat cgccaggata atcaacattt gcaccatttc
     2461 agcgacgaac gtgacggcac cggcggtaat ggggttaaag actttatcag tgcgcccgcg
     2521 acggatcagg atgctgtgta ccaggccgcc gagcaatcct tcaacgatgg tcgagatcat
     2581 gcaacttagc gcggtcatgc cccccatcga atatcgatgt aacccgccgg tcagaccaac
     2641 cagcccaccg acgaccggac cgccgagtaa gccgcccatt accgcaccta tcgcacgggt
     2701 attggcaata gaatcgtcaa tgtgcaaccc aaaccaggtg cccatgatgc agaagatgga
     2761 aaagacgatg tagcagagaa atttatgcgg cagacgaacc gtgacctgca ttaacggtat
     2821 gaataatggc gttttactca ttaaccacgc aatgactaaa aaaacgcaca tctgctgaag
     2881 cagcagcaac accagattaa aatcgtacat acccgcaaac cacacttccc tttaaaacgc
     2941 gtaacataca ttgcttgcgt ttaactttct ttgaactctt gcagaaaaat gagaattcgt
     3001 gagtacgatc actcaaaatc gcctggcaaa aataaaatca ccctatagat gcacaaaaaa
     3061 cgggcaaaac tacctggttc gcaaaactgc gtctaaagtt aaaccgggac ctcgcgagca
     3121 agggtgagac gatggcgctt tacacaattg gtgaagtggc gttgctttgt gatattaacc
     3181 ctgtcacgtt acgcgcgtga cgcaacattt ttaaaaatcg acgaagatcg tccaggagcg
     3241 ccgtttttca agggttggat agatgattcg tttaccatgt cgacgaactt cgacgaattt
     3301 cgccgataaa aagtgtccca tacatgcccc attgatgccc cataaacaat atcaggaatt
     3361 gacactggtt atccatacag acaaaaataa cacctgtaca taaccacagt tacaggaggg
     3421 tgttatgcgt gttgagatct gtatcgctaa agaaaaaatc agcaaaatgc caaaaggttc
     3481 tgtttctgcc ctgcagcaag aaatgcttcg acgtgtcagt aaacgttatg acgatgtgga
     3541 cgtgattgtc agagccgcca acaatgacag tctcaccatt ttaagagctg tagataaaga
     3601 atccgcgcac gaatttgttc aggaaacact gcaggaagcc tgggaaaccg ctgacgactg
     3661 gtttattcgg taaatcttct ttacggggag ttaacttact ccccattttt tattgcatga
     3721 taattgcagc aacggcacag agtgtcacca caccacccag ccacacaaac agcatgaagc
     3781 acattgctcc cgctgtataa cgttttctcc cgtacatacg gcatttgtct ttattcatca
     3841 tctgcccctg aatcagctga agacgctgca ggtccggtaa cactgaatca ctccgcacac
     3901 gcaagatgtt t
//
LOCUS       DAZFFE010000205         3877 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFFE010000205 DAZFFE010000000
VERSION     DAZFFE010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.207"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            35..3585
                     /locus_tag="ACHJ69_004761"
                     /pseudo
     CDS             35..3585
                     /locus_tag="ACHJ69_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773871.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
     gene            complement(3622..>3877)
                     /locus_tag="ACHJ69_004762"
     CDS             complement(3622..>3877)
                     /locus_tag="ACHJ69_004762"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HFO5017555.1"
                     /translation="VKPPFLIMHGSKDTLVSPEQSAKLFRMLKKNGDNAEYVLVEGAE
                     HGDKTWYQPIIINRVVEWFTKNLGAPIKTAPQQQNPNANL"
BASE COUNT         1096 a          751 c          937 g         1093 t
ORIGIN      
        1 tttttttcat tatattcata acgttgattg aactatgacc actgtgaata aaaagttaaa
       61 aaaactgcat ctggcgcgat tacatggtcg gtaattgtta cccagatatt atctccagtt
      121 tctctttctt tgatcccagc aaacagtttt gcatcatctg gtaataaaga tgttacgcaa
      181 acttatgcca gcgatgagca tgcaaataaa gtggcctcat ttgcagcaag tgcaggtcag
      241 agcctagcga ataataatgc aagtagtttt gctgtaaata ctttatcaac tcaggcgaca
      301 aaagaggtcg tcgactggtt gcaacaatat ggcaatgcgc gcatcaagct taatgtcgat
      361 gattcttttt ccttgaagga ttcatcattc gactttttat atccatggct ggatactcag
      421 gattatgtgt tattcagtca gacatcacta catcgtactg atgaccgaaa tcagaccaat
      481 attggtttgg ggattcgtca tttcactcct gataacgcaa tgttgggtgc gaatgttttc
      541 tatgattacg atttaagtcg cagtcactct cgtgcaggtt ttggggttga gtactggagg
      601 gattatttca ggcttggtgt aaatacctat tttggtctat ctgactggaa gaacagtcgg
      661 gatattgatg attatctgga aagacctgca aatggctggg atttttctgc tgaaggatgg
      721 ctacctgctt atccgcaatt aggggcatct attcagtttg aaaaatatta tggtaaaaat
      781 gtcgggttat ttggaagcga taaccttcag gaaaaccctt acgcagttac tgggggaatt
      841 tcttatacac cagttcctct gattaagttt tctgcacagc ataagcaagg acagagcaac
      901 gttcacgata caaccttcgg tgttgagttt aattatcgcc cgggcgtttc cctggctgaa
      961 cagcttagca gcgataatgt tgcagttatg cgagaagtcc agaatcggcg ttatgatttt
     1021 gttgaacgaa ataacaacat tgttctggag tacaagaaga agcatgcact gaaaatcagt
     1081 ttgccagagt ctgttcaggg ggagggggaa tcaatcatcc ctgtaacact gacaatcaac
     1141 aatgctagtg gtggtatcaa gtctgtacag tggaatgata gtgcattcac tgcggctggc
     1201 ggcaagatca gtggaaatgg cacatcatgg cagatcactt taccggctta taaaagcgaa
     1261 ggtgtaaatt cgtggaatgt tggagccaca gtccaggata acagaggcaa tgtttccaac
     1321 tatgccgtta tgaatatcag cgttaataat agtggtgtct cgacagcgga ttcatctttt
     1381 acgctggatg gggattcgtc tccgacgatc tctgctgatg gtcaatccac ttatccaata
     1441 gtattaagcc tgaaagacag taacggtaag gcattaaccg gactggctga taacattgaa
     1501 atgtctgtgg aatttactgc ggatagcaat agtgctcgac agcgtgaaac ggtaactgct
     1561 ccgtcattag gcgcggtaga ggaaatctct gctggtgtat atcgctctgt tctggctgct
     1621 ggttcgcagg ctgggacggt acgtgtaact gcaaaagttc agggaaaaac atttactctc
     1681 agtattaagc aaacagcagc aacagaacct gattctgagg tcagtgctgt tttaaccgct
     1741 gcaccagcag aacaggtagt tggttacaat atcaatctac aactggcagt gaaggattca
     1801 caaggaaatg caatcactgg tgataacact ctgagctttt atgctcttaa ccaggcagaa
     1861 ggagtcgagt ttgggactgt aacggaaaaa gacggtgtct atagcgcgac agtaacttca
     1921 aaacgagctg gtaagattac tatcggtgtt aaatctgata gtcatgattt ctcaggtgtt
     1981 aagaaagaaa tcacctttat tgaggaccgg cagcaggtta cattctctca gtttgaagcc
     2041 agccaaaata atgcattggc tgatggaaaa cagagaaata tggtaactgt aagccttgcg
     2101 gaccgtttcg gcaatgttgt tcctggttat gctgtcacat tgtctttacc agcagggata
     2161 actcaagttg gtggtgaaca tgctgtttca actgacgaaa atggtaatgc tatttttgcg
     2221 ttaataagct ccactccggg gtcttacgtg attacagctc atgctggttc tcagatgtct
     2281 actgagttaa cagtgacctt tgcatcaaat atgaccggag cttcactgtc actgacgcct
     2341 gaaagcagta gtttaatttc caatattcct gctaatggta aggatgcagc ggtattagat
     2401 gtgcagctga caaatacgaa tgcgtcggtc aatgggcaaa agattcagct catcacctca
     2461 tctgaaggat tatctgtacc aacaaatatc gtgactgatt caacagggca tgtttctgtt
     2521 ccgcttacaa cggtgaaagc tggtgagtac acggtgactg cacgggtgac tgacggttcc
     2581 catagcgtgg aatctggcag cgttaagtta acttttgtgc ccgatgttgc ttctgcagaa
     2641 cttactatga gtgtatcaaa acaagagatt gtggccgatg gcagtgaaaa cgcaacagta
     2701 aacattcagt tggttgatgc gaataacaat gcgtttacag gcgaggtgga tttaacgatt
     2761 acaccatcga cgggagcatc attgaccagt agtaatctgc aactggatgc tcatggacag
     2821 gcaacaacgc agttcactgc atcgaaatca ggccagtaca caattcaggc cgagtatgtt
     2881 ctggatggga aacgcataac cgcaagccag aatattgatg ccgtgacgga tgtgaagggg
     2941 gctgtgttgg agatcacatc atctgcatct tcggctgtag tgagcgatac caacaatctg
     3001 gtatttacgt tactgttgaa aagtaagtca ggtgaagcat taagtggtcg agcattgaat
     3061 gttaagacat ctgggccgtc taaatatggt gcgttggtgg ttgataaaac cacagtaacg
     3121 actgatgaga acggtcaggc tacggtaagc gtacatggtc gaacggttgg ttcgtataaa
     3181 ttaacggcga ccttaaacga acttggcagt gatgttagcg cggtgaaatc attctccctg
     3241 tacgctgatg acgcaaatgg agtattgact ctaacaaaag atcctggtta tgagacaaac
     3301 gatggctctc cagtaggtat ttatgctcga tttgtcgatc attttggaaa cccattatct
     3361 ggtacggttg agttttctgc tggtagtgaa gatagtccag actctcaacg ggtcaaaatg
     3421 gagcctgcta cagtaacgtt acactggaca ggtaatgcag cttcggaatt cagcacctat
     3481 gaaagtggtt atcactggat taaggccaaa ataacgaata gcaaaaatac atatgagaaa
     3541 acaataagga cttatgttgt aaaactacct gaaaaggata gttaacaaaa ttgaatgggg
     3601 agggcttagc cctccctctt tttacaggtt agcgtttggg ttttgttgtt ggggagctgt
     3661 ttttataggc gctcccaggt ttttagtaaa ccactcaacg actctgttta taataattgg
     3721 ctgataccat gtcttatcgc catgctcggc cccttctacc agcacgtact cagcgttatc
     3781 gccgttcttc ttcaacatcc tgaatagttt ggcgctttgc tcaggtgaaa ccagagtgtc
     3841 tttgctacca tgcataataa gaaatggggg ttttact
//
LOCUS       DAZFFE010000206         3824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZFFE010000206 DAZFFE010000000
VERSION     DAZFFE010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.208"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            170..1408
                     /gene="alaC"
                     /locus_tag="ACHJ69_004763"
     CDS             170..1408
                     /gene="alaC"
                     /locus_tag="ACHJ69_004763"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO5017556.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLYTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            complement(1473..1544)
                     /gene="ypdK"
                     /locus_tag="ACHJ69_004764"
     CDS             complement(1473..1544)
                     /gene="ypdK"
                     /locus_tag="ACHJ69_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO5017557.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(1900..2820)
                     /gene="lpxP"
                     /locus_tag="ACHJ69_004765"
     CDS             complement(1900..2820)
                     /gene="lpxP"
                     /locus_tag="ACHJ69_004765"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO5017558.1"
                     /translation="MFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GTMARPFLKRRESIARKNLELCFPQHSAKEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            3173..3415
                     /gene="yfdY"
                     /locus_tag="ACHJ69_004766"
     CDS             3173..3415
                     /gene="yfdY"
                     /locus_tag="ACHJ69_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO5017559.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNMVSAISSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(3492..3767)
                     /gene="ypdI"
                     /locus_tag="ACHJ69_004767"
     CDS             complement(3492..3767)
                     /gene="ypdI"
                     /locus_tag="ACHJ69_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFO5017560.1"
                     /translation="MKVNLMLFSLFLFVSIMACNVFAFSISGGGSEASYKETEKTSVM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEDITPTYPTKANYLIHPLR"
BASE COUNT          979 a          894 c          961 g          990 t
ORIGIN      
        1 gcgtagataa atttcctgtt tttgaagtgg agttcctcat ttaaagccat tgataaaaat
       61 gttctaaaac gctggacaga cagaaattaa tcaggctatt ttcaaaacag ccaccatcgt
      121 ggcgacgtcg ctcggacggt ccgggcgcta acgttaatct gaggaaatta tggctgacac
      181 tcgccctgaa cgtcgcttta cgcgtattga tcgtctcccg ccctacgttt ttaacattac
      241 cgctgaactg aaaatggctg cgcgtcggcg cggcgaagat attatcgatt tcagtatggg
      301 taacccggac ggtgcgactc cgccgcatat cgtcgaaaaa ttatatacgg tggcccagcg
      361 cccggacacg catggttact ccacttcacg cggcattccg cggttacgtc gcgccatttc
      421 ccgctggtat caggatcgct acgacgttga aatcgacccg gaatcagaag ccatcgtcac
      481 tattggttcg aaagagggcc tggcgcatct gatgctggcg acactggatc acggtgacac
      541 agtgctggtg ccgaatccaa gttaccccat tcatatttac ggcgcggtga ttgccggggc
      601 gcaggtacgc tcagtgccgc tggtggaagg tgtcgatttc ttcaacgaac tggaacgcgc
      661 cattcgcgaa agttatccga aaccgaagat gatgatcctc ggcttcccgt cgaacccaac
      721 cgcgcaatgc gtggagctgg agttcttcga aaaggttgtg gcgctggcga aacgctacga
      781 tgtgctggtg gtccacgacc tggcctatgc ggacatcgtc tacgatggct ggaaagcgcc
      841 gtcaatcatg caggtgccag gtgcacgcga cgtggcggtc gagttcttta cgctgtcgaa
      901 aagctacaac atggcgggct ggcgtatcgg ctttatggtc ggtaacaaaa cgctggtcag
      961 cgcgttggca cgtattaaaa gctatcacga ttacggcacc tttacgccgt tgcaggtggc
     1021 ggctattgcg gcgctggagg gcgatcaaca gtgcgtgcgc gatattgccg aacagtacaa
     1081 acgccgccgc gatgtgctgg tgaaagggct gcatgaagcg ggctggatgg tcgaaatgcc
     1141 gaaggcttcg atgtatgtct gggcgaaaat cccggaaccg tatgctgcca tgggctcgct
     1201 ggaatttgcc aagaaattgc ttaacgaagc gaaggtctgt gtctctccag ggattggctt
     1261 tggcgactac ggcgacacgc atgtgcgctt tgcactgatt gaaaaccgcg accgtattcg
     1321 tcaggcgatt cgtgggatta aagcgatgtt ccgtgccgac ggtttactac ctgccagcag
     1381 caaacatatt cacgaaaacg cggaataaac accatcgtca gaaatgcaaa caggagccag
     1441 atggctcctg tttttcattg cacagtttgc ttttagatca tcagggcaaa agtaccggcc
     1501 caaacgatga ccataaaaga aatgcccata aagaaatatt tcactttaca tctctccgcg
     1561 taactactgt tacgcctgaa tgactttatt acacctgata cagtgagttg acaccgggca
     1621 aaacgacctt aaacgtcctg tattttaccg tgggcatcat cccagaattc tgagctaaac
     1681 tgctcgagac ggcgctaatg tacgcctaaa actgaaacga gacaagaggc attttcttat
     1741 ttacttttag taacttacag gtgtcttata tcggcgacag tttaatttct gacggtaata
     1801 attagatatt cagcgaagga aaaaacctta agaaaaaagc cataaaaacc atgaggttat
     1861 tatggctgat ttgaggaggg aaagagtgag agccgtttgt taaatgtata acgacgattc
     1921 acccaccggg cgcgttttaa agcgacggtg gatccagagg tactgctccg gtgcgcgcat
     1981 gatctctttc tcgataatct tgttcatata ggcagcggct tgattttcat ctgtcgggta
     2041 gccttccatc tctggggtga tgtacaaacg atatccgctg taatccgctt ttcttaccat
     2101 cgttacggtc aacatggctg cgccagagag acgagagagg acataggtgc cattggttgt
     2161 ggcgacattt tccaccgcaa agaacggcgc gaaggagctg cctttacgac cataatcctg
     2221 atcgggagca aaccataccg cttcaccttt cttcagtgca ctgacaatac cgcgcagatt
     2281 atttctgccg atcatcgctt tgttagagcg catgcgacca cgggtctgca cccattccat
     2341 cagctgatta ttatgcgggc gataggtggc catcattggt tggcaaagtc ccatcacccg
     2401 accgcccagt tccagcgaca taaaatggac gccgacaacc attacgccgc gattttgcat
     2461 ttgtgcgcgt ttaaggttat ccagcccttc cacatcaaac catttacgta cgcgactgtc
     2521 gggccagaac cacgccatgc cggtttctac cagcgccatg ccgagtgaac gaaagttttc
     2581 ggcaatcatc ttctcgcgct ctttcgcaga atgctgcggg aaacaaagtt ccaggttttt
     2641 acgggcgata gattcacgac gtttcaggaa tggtcgcgcc attgtgccga tacgcgtgcc
     2701 gagaaagcag agaacaggat aaggaagctg tacccaaagc cagagtacac caagcccaaa
     2761 ccacgtgagc cagtagcgag gatgcagaaa ctcgcgggaa aatttgcatt ttggaaacat
     2821 agttaccttc tgtagatacg atttgttgta tcgcagaaga ttccatgtcg acagcggtac
     2881 actcaattga ttaggccatc atgtacgaca atggttcctg taaagagtgt aaagactagt
     2941 caggacataa ccacgaggag catgaatgcg gtatctgtac tttatcactc gctgactgct
     3001 ttcagctact gattattaat cgtgcaaagt aaatattttt ccgtacacaa cagtcttgca
     3061 tttaacatgc tgttttcatt gatgcttagc aaaaattgcc agaggttaag gattgtctta
     3121 ctattaaagt gactgaagtt acaggttttt tagcgcggca ggaggttgag ctatgattta
     3181 tttatggatg tttctcgccc tgtgtattgt ctgcgtaagc ggatacatcg gtcaggtact
     3241 gaacatggta tcggcgatct cttctttctt cggcatggtg atcctcgctg cactcattta
     3301 ttacttcacc atgtggttaa ctggcggtaa tgaactggtg acggggatat ttatgtttct
     3361 tgccccggct tgtggcttga tgattcgctt tatggtgggg tatggcaggc gatagtgggt
     3421 caggatggct catttgggca ggtgttcaca tcagcatcag acatgattgt tcacctgcca
     3481 ggtgacagac gctatcgcaa aggatgaatg agataattcg cctttgtcgg ataagtaggc
     3541 gtgatgtctt ctgtgaggtt taatggtggt gcatcttctc tcatcttaaa caaaatcgcc
     3601 tgtgatggct gaagttttgt agaatgcgtt gtcgtcatca ctgacgtttt ttctgtctct
     3661 ttatagctcg cctcactacc accaccagaa atagaaaatg caaaaacatt gcatgccata
     3721 atagagacaa ataaaaataa gctaaaaagc attaagttta ctttcataga aaatatcctg
     3781 aaaatggttc actgaaaacc tacgatgtta tggctgtatt tata
//
LOCUS       DAZFFE010000207         3777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFFE010000207 DAZFFE010000000
VERSION     DAZFFE010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.209"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..3435
                     /locus_tag="ACHJ69_004768"
                     /pseudo
     CDS             1..3435
                     /locus_tag="ACHJ69_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
     gene            3586..>3777
                     /locus_tag="ACHJ69_004769"
     CDS             3586..>3777
                     /locus_tag="ACHJ69_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HFO5017561.1"
                     /translation="MAAYLLKKHGGSMRFIKLMYLSDRKAMELYGFPISGDHYFSMNK
                     GPVLSQTYSLVKDGSENEYS"
BASE COUNT          982 a          858 c         1124 g          813 t
ORIGIN      
        1 ttgttcgcga aactattcgc acgttaggcc agcgtgtgcg ggaaggttac acgccgcgtg
       61 aggatggcgc tggcgcatcg tctcagtact cccgtagcgg taagcctgat ccgttcaaag
      121 tgccggaagg tgagggcgag cgttatcgtg atgaccttgc cagaatgatg aagtctctgc
      181 gcaccacaga tttaacggta aacatcgggc gtacgccgcc ggtattgcgt caccttggcg
      241 caccagattt gccgctggtt atctcccgcg acactgtgcg taaggccacc aatggagtga
      301 aacatgtggt gccgatggat gttatcgaga gactaccgga acttatgcac gatccggatg
      361 caatttaccg ttccgcgaca gaaagaaatg cggttgtgat gctgcttgat gccatggata
      421 aaaatggtga tcctgtagtg tcagcggtac acatgaaggc tgtccggtcg cgcctggaaa
      481 tcaacaaggt agcttcggtt tacggtacgg aaggaggaat gaataaggca aatagtctgg
      541 ataaagccgg attaacgctt taccggaaga aaaaattaag ccgcgataac cttctgcaca
      601 gagggcttca attgcccaaa ggggaacatt cttatcgcgg ctctgtagat aaaatactct
      661 atcctgaaga tattcgcaag gggccgtatt actcccgtac cagcagtctg acatcggaag
      721 agacaattgc atcccgtttt gttcgccaga tgcaggataa attccaggtg ctgaaggcgg
      781 tgcaggagaa tatccgtaaa actggcggaa aaatagatgg cagcaataac gcctatatgg
      841 ctgaagaact cttccacggg aaggcggaaa acgacctgaa cgtaatgaag gagcgctacg
      901 ttcagccgct ggccaaattg ctggcggact acaaaattgc gcaggccgat ctggatgagt
      961 acctctacgc ccgccacgcg ccggaacgta acgcgcatat tgcgaaaatc aacccgaaaa
     1021 tgccggacgg cggttcgggg atgaccaacg cggaagcggc ggaaatcatg cagcgtgtgc
     1081 gtaacagcgg caaacaggca cagtatgacc gtctggcagg gattattgac gatatgctgg
     1141 cccgtcgccg tgagattatc cgtgaggcag gactggaaga gagcggcgtg gtggatgcct
     1201 ggcagaaagc ctaccgttac tacgtccccc tgaaagggca ggatgttgac ggtgtggtgt
     1261 cactgccacg tacaggcaag ggattcacca tcggcgggcg tgaaagtaag caggccatgg
     1321 ggcgtgcatc ccgcgcacag tctccttcca ctcaggcgat acaggatttg agtgaatcgc
     1381 tgatccgcaa tcgcaaaaat gaagtgggta acgccttcct gaaactggta caggataatc
     1441 ccgacaagga ttactggcag gtattcactg atgacagacc ggatacaatg cgtgtgattg
     1501 cagagcgcaa ggaccaggaa actggtgaaa ccattcgcga agttgtcgag cgccctgtgc
     1561 cgatggcaat gatggcggat cgctacttca ccaccaaaaa ggacggcaaa acttactaca
     1621 ttaaacttca tgatccgcgc ctgatgcgtg cgatgaagaa catggggccg gaaaccagta
     1681 acgccgtaat ccgtacgctg gggaaagtta accgtttcct ggcaacggtg aacacgtcgt
     1741 ataacccgga atttctggtc agtaacttca tccgtgacgt gcagacggcg gtgatgaacc
     1801 tgaaggcgga gcagggaagg agcgacggta aactgaaagg gctggataac ttatccgccc
     1861 tggctgtggt gaaagacagc cggtctgcca tgtctgccgt atacgccagt ctgcgtggta
     1921 aaaccctcac gggcaacggt gcgcagtggc agaaggtgtg gaaagagttt gttgaggacg
     1981 gaggtaaaac cggctggttt aacatgggtg accttgaagg ccagcagaag gaaatggatc
     2041 gccttgtatc gctggcgaag ggaggatgga aaggccagag tatcggtgca tggaattcgt
     2101 tccttaacct tgtcgaggat gccaacgggg cggttgaaaa cgctctgcgt ctttctgcct
     2161 ataaacacgc ccgtgatgct ggtttgtcac gccagcaggc ggcgtctctt gccaaaaaca
     2221 tgacggtgaa ctttaaccgt cgtggtgagc agggagcgct gatgaactcg ctgtatatgt
     2281 ttgccaacgc cagcattcag gggaccgcaa acctggtgag aacgctcgga catcttaatg
     2341 gcgacgggcc gttactggag cgccttcgct ggaagaatct caatgtaccg cagaaaatcg
     2401 cgcttgcagc tgtgggagcg ggttatctgc ttggctcgct taaccgcagt gtggcgggtg
     2461 aggatgatga cggggttaac tggtacgaca aggtgccgta tcatgtgaaa gagcgtaacc
     2521 tcgtcattat gaaatcgatg ttcggggggc agggccggag agtactggag tattcctctg
     2581 ccttacgggt acaacgtttt cttcctgctc gggcatactg ctgaaggtgt ggcagcgggc
     2641 gacctgactg cttcacgtgc tgccggtaat gttgtcggtg gtatcctggg ggcattcagt
     2701 cctgtgggca gtgaaacgtc ggaaacactg tccggggcat tgctgaaaaa tgcagcgccg
     2761 accattctgc gtccgtttgc gaaccttgcc atgaatgaaa acttcatggg ggcgcagatt
     2821 taccaggaga acatgccatt tggcacactg aaaccggaca gccagctggg cagacgttca
     2881 acgcctgaga cgtacaaggc gtttgcatcc tggctgaatg cgttctcagg tggcagccag
     2941 tatcgtcccg gcgcggtgga tatcacaccg gaatcgctga aattctggat tgactatatc
     3001 tccggaggga cagggcgctt catttccaaa accacagatg cggcggtgaa atcgctgaac
     3061 ggtattgata taccggagca gcaggtgccc ttcctgggga aaatttcggg tgaggtgatg
     3121 ccgtatgcag accagcagaa gatgtacgac cggatgacag aggttgcgca gtatcacgca
     3181 gagctgaaga gtctgaccgg tgcagaaaga acggcgttca ttgacgagaa caacggaaaa
     3241 ttgttgatga acgggcttat gcaggatacc cggaagagac tgaaggattt gcgcaaacag
     3301 cgcgatgcca tttatgccga cagttctctc agtctggcgc agcaggcggc gatggtgaaa
     3361 tcggtagagc gggatatgaa ggttgccgtg gatcgcttta accgcgagta caacaaaaaa
     3421 gtgggagtgg attaacagaa atggccccgt acggaagtgc ggggctgatt aagaaataaa
     3481 cacacattaa cctgtaataa ccggagctat taacatatag tcagaaagag tatttcatgt
     3541 gagacagaga gccgatttat gtttaatgaa gaaaaagttg cgtaaatggc agcgtatttg
     3601 ctgaaaaaac atggcggatc tatgcgtttc attaagctga tgtatctctc tgaccgcaaa
     3661 gcaatggagt tgtatggttt tcccattagt ggtgatcatt atttctctat gaacaaagga
     3721 cccgttttat cgcagacata ttctttggtt aaggatggaa gtgaaaacga gtacagt
//
LOCUS       DAZFFE010000208         3692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFFE010000208 DAZFFE010000000
VERSION     DAZFFE010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.210"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1290
                     /locus_tag="ACHJ69_004770"
     CDS             <1..1290
                     /locus_tag="ACHJ69_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001767924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HFO5017562.1"
                     /translation="PGYSGIRLDINSDRKELFKSPAFRYLTSLRRAEMRLTTAAITLF
                     SINKKIPAIRLPNAINHYSNYLKNLEDLATSSGINSEAFITKSKAFNTVIRRALGCKL
                     RTYISKNYSDLSFVCDVPLDWIRFNNIPIMFSHEISRINATPGNVLLQSASAFPRVLV
                     KASELRKVLVIRSFEPDDHLKFILENAIEIFKKQMPDLDCEIIDVRSKAEFIDALNQY
                     QGHVLVMDCHGNHDGNGSHGWLIIGEDKVDTWSLRKIARIPPIVILSACLTSALSGSH
                     ASVANGFVISGALSVIGTLLPVNAIDSAVFVSRLIYRFYEFPSTLSTNFTHVNVRLFL
                     SVFLRMSYASDLIRGFLSEDLIPNNSWRKDAVDINMYINMLHHDWYDYVINKLTMLTG
                     LTKKDVLDFIDRKLFITETMCYSQIGFPDAITISLKD"
     gene            complement(1592..1831)
                     /locus_tag="ACHJ69_004771"
                     /pseudo
     CDS             complement(1592..1831)
                     /locus_tag="ACHJ69_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            complement(2212..2574)
                     /locus_tag="ACHJ69_004772"
     CDS             complement(2212..2574)
                     /locus_tag="ACHJ69_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017563.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRTFDALEAKCAA
                     LENKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVIPKAPEAGA"
     gene            complement(2565..3101)
                     /gene="yfdR"
                     /locus_tag="ACHJ69_004773"
     CDS             complement(2565..3101)
                     /gene="yfdR"
                     /locus_tag="ACHJ69_004773"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO5017564.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKQMEEKIDSVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLSPGHA
                     YGMFMERFNELSELRKCA"
     gene            complement(3229..>3692)
                     /locus_tag="ACHJ69_004774"
     CDS             complement(3229..>3692)
                     /locus_tag="ACHJ69_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HFO5017565.1"
                     /translation="LLSVNGERNSQKSLAEWIEDWADYLVGFDANGDTIQATKAAAAV
                     RKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCIPFEGLKERPFKLR
                     LSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
BASE COUNT         1011 a          819 c          760 g         1102 t
ORIGIN      
        1 cctggctatt ctggaattag acttgatata aattcagata gaaaagaact ttttaaaagt
       61 cctgctttcc gttacctcac ctcactaaga cgcgctgaaa tgcgattaac aacagctgcg
      121 attactttat ttagcataaa taaaaaaatc ccagcaataa gattgccaaa tgcaataaat
      181 cattactcca attatttgaa aaatcttgaa gatcttgcaa cttcttctgg gataaatagt
      241 gaagcattca tcactaaatc taaagcattc aatactgtta ttcgaagagc ccttggttgc
      301 aagttgagaa catatataag taaaaactat agcgatttgt cctttgtttg tgatgtacca
      361 ttggattgga ttaggtttaa taacatacct ataatgttca gtcatgagat atcaagaata
      421 aatgctacgc ctggtaacgt tcttctacag agcgcctctg cttttccgcg cgttttagta
      481 aaagcatctg agttgagaaa ggttttagtt atacgttctt ttgagcctga tgatcactta
      541 aagttcatat tagagaacgc aattgagata tttaaaaaac agatgccaga tcttgattgc
      601 gagattattg atgttagatc gaaagctgag tttattgatg ctctaaatca atatcaaggt
      661 catgttcttg ttatggattg tcatggaaac catgatggca atgggagtca tggatggtta
      721 ataataggtg aggataaagt ggacacttgg agtttgagaa aaatagcaag aattcctcct
      781 atcgtaattc tgagtgcatg tttaacatct gccctaagcg gttctcatgc ctctgttgct
      841 aatggcttcg ttataagtgg tgcattaagt gtcatcggaa cattactgcc agtaaatgcg
      901 atagactctg ctgtgtttgt aagtcggttg atctaccggt tttacgagtt tccttcgacg
      961 ctatctacaa attttacaca tgttaatgta aggctttttc tttctgtttt tcttaggatg
     1021 tcctatgcaa gcgatttaat aagaggattt ttatctgaag atttgatacc gaataattca
     1081 tggaggaaag atgcagttga tattaacatg tatataaaca tgcttcacca tgattggtat
     1141 gattatgtaa ttaacaaatt gacgatgtta acggggctca cgaaaaaaga tgtacttgat
     1201 tttatcgata gaaaattatt cataaccgaa actatgtgct atagccaaat tggatttcca
     1261 gatgcaatca caattagtct taaagattga caactgtatg taataaatat ggcatacttc
     1321 cggcacagag cgaatataac agatcaggtt tagctctgtg ccataggtgt accaactctc
     1381 atctgagcta atgctcgtta ctcaataact cccgcaaatt tgtaaatctt gcgtgatgcc
     1441 catttatttg ggcatgattt aatatcagga tctggaaagt ctggcctgta tttctggcca
     1501 gttctcctgt ttacgctgtt ccagcgaaga acggtcgata ctgaaacgcc acagaagtcg
     1561 gcgacttgtt tagttgtcat taagttgttc attacttcac ctcctgcggt ggctccggta
     1621 gcggcatcca gtgggttact ttcgatgccg gttcttcccc atcgtcagta actgcccacc
     1681 atttgtttct cgaccaatcg taataccctt cgaaggtatc gcactcagtc cagccgtaag
     1741 acttacccca acaccaaaca tactgtttat cgtccggcat tcgctcacta cagcttatcc
     1801 aaccatccgg agttaccgga gagttgcccg acagctcgtt caacttgtaa gtctggctta
     1861 caggttcggc accatgaagc atggcggcgc ggcaggcgtt ccagccttca tcaaagccga
     1921 ctatgccatt atttaaagac ggacgaacat ctggcaccat cggcactggc ttggctatat
     1981 atagcggctg aacataccag ccctttgata accaactgtc agcaatgttt ttactccgtg
     2041 ttattgccgg aatacctaag ccattgtctg aatgcagcca tgccaccggt tctgcttcca
     2101 gcgataccaa agcaatttca taagcacggc gctcaatatt gtcttgcaca tccagactgc
     2161 ctatgcgctc tttgatttct ttaatcagtt ctttgtcggt aaatgtggtc attatgcccc
     2221 tgcctccggt gcctttggta ttactgccca gtgagtgata ttgacgtttt caagatcccc
     2281 gacctgaaat gtccactgcc attctccggt ttccttttgt ccccaggtgt accagagaga
     2341 acgccagcca atcagccagc cttctccgtt agcatcaaat aacagaacac tttcatttgc
     2401 aggtggcaat tcagctgaca ctggtattat tttgttttcc agtgccgcac atttagcttc
     2461 aagcgcgtcg aatgtacgta ctaggtactc agcatttgtt tcgttcactt tcagatctcg
     2521 cggtacacat ttcccgcgaa gaaacccttc catttcgaaa acattcatgc gcatttgcgt
     2581 aactccgata actcgttaaa acgttccata aacatcccgt aggcatgacc tggcgacagt
     2641 ggaataactt tgaacatctc tgttgccggg ataccttcca atacaggcca gaaagagcca
     2701 tcatcaagtc cgagatcgcg gcgttcggtt gccagcataa tgagatcggc atatttcacg
     2761 ggcgtactca taactggggg taatccgtat ttctcacgga ttacggagtc tattttttct
     2821 tccatttgtt tatagtcagg aagaaggcgt ttcagtggtg cgggaatgtc ctggcaatac
     2881 gcttctgttg catcatgcat taacgcttca aaagcaaatt cctgcggcac cagctggctg
     2941 caaagaaccg catgttgggc gacgctgtag aagtgcgaaa gatgaccggc aaagcgacag
     3001 atatttgaaa gggaaaccgc gatatcgtta atatcgatgt cgtctttatt tatcctgtca
     3061 taataaaaat gcttcccgga aaaagtttta ataaatgaca ttttgttctc cacgtatatg
     3121 cgctgcaccg cgctgaattc tggtaaaaag aatccctcac catccggcga ttattgagta
     3181 aattacgttt ccataaatgc ccccgcaggg gcatttgcag taatgaaatc aggcggtgaa
     3241 agtaccaata aaggtttcta ctttgctgtc cttgaatttc tcaacaagca gatcacgaaa
     3301 ttcgttagcc atttcttcct gcaccgcctc cagctgtata atgcgcagaa ccagtaccgg
     3361 acgatcgcca gtgataatgc tgaggcgtaa tttaaacgga cgttctttca gaccttcaaa
     3421 cggaatgcat ttaaattcaa atgccactgg cataatgtct ttggtcttcg cttcgacaga
     3481 ctccatcagg gagcgtttgc cgctgaagtc attatcttca aaatcagcgg tctggtttgc
     3541 ttcaatcgtg attttacgga ccgccgcagc cgcttttgtt gcctgaatgg tgtcaccatt
     3601 agcatcaaag cccacaaggt agtcggccca gtcttcaatc cattctgcca gtgatttctg
     3661 ggagttacgc tcgccattaa cagacaacag ag
//
LOCUS       DAZFFE010000209         3432 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZFFE010000209 DAZFFE010000000
VERSION     DAZFFE010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.211"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..354)
                     /gene="bglB"
                     /locus_tag="ACHJ69_004775"
     CDS             complement(<1..354)
                     /gene="bglB"
                     /locus_tag="ACHJ69_004775"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFO5017566.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYERLFDEMAQAGI"
     gene            complement(373..2247)
                     /gene="bglF"
                     /locus_tag="ACHJ69_004776"
     CDS             complement(373..2247)
                     /gene="bglF"
                     /locus_tag="ACHJ69_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFO5017567.1"
                     /translation="MTELARTIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNSFTAMVIGGALVHPLILTAFENGQKVDALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPITFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLIPLIINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPGIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMFH
                     FITAKSQPAQVAQQEKTPEIITPEQGGICSPMTGDIVPLIHVADTTFASGLLGKGIAI
                     LPSVGEVRSPVTGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVG
                     DKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQINAGEPLLSIIR
                     "
     gene            complement(2381..3217)
                     /gene="bglG"
                     /locus_tag="ACHJ69_004777"
     CDS             complement(2381..3217)
                     /gene="bglG"
                     /locus_tag="ACHJ69_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFO5017568.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRPGERINSS
                     GIEKEYALSSHELNGRLSELLSHMPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRIIE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT          940 a          882 c          733 g          877 t
ORIGIN      
        1 gatccccgcc tgcgccattt catcaaacag ccgctcgtaa aacgctaacc ccgcttcatt
       61 cggttcggct tcgtcgccct gaggaaaaat tcgcgcccag gcaatggaaa tacgcagaca
      121 ggtgaagccc atctcggcaa ataacgcgat atcttccggg taacggtgat aaaaatcgat
      181 ggcgacatct ttgatattct ctttccccag gatgcgcggt tccatttttc ccattacgcc
      241 atgaggctgt aaatctgagg tcgtgatccc tttgccatct tcctgccagg cgccttccac
      301 ctgattggca gctgttgcac caccccaaag aaatgtttct ggaaatgctt tcataattaa
      361 ctccttttat cgttagcgaa tgatggataa cagcggctca ccagcgttta tctgcgccgt
      421 gccgtggggt aatacgtccg taaaatcatc gctattactg attaataccg gcgtcgtcag
      481 atcaaatccg gcctcgcgaa tagcagggat atcaaaagaa atcagccgat cgcctgtatt
      541 gaccttgtca cccacattga cgtgagcgga aaagaatttg ccgtccagtt ttacggtatc
      601 gataccgaca tgaatcagga tctccacgcc atcatctgac tcaatgccaa tggcgtgtaa
      661 tgtggcgaac aacgaagcaa ttcgacccgt aactggagaa cgtacttcac caacagaggg
      721 cagaatggca atacctttac ccaacaggcc gctggcaaac gtggtatcag cgacgtgaat
      781 gagcggcaca atatctcccg tcatcggtga acagataccg ccctgctcag gtgtaataat
      841 ctctggtgtt ttctcttgct gggcaacttg cgctggctga ctcttagcgg tgatgaaatg
      901 aaacatcacc gtaccgacaa atgcgcaacc gatggcaatg acaccgccaa taacgctggc
      961 ccatacggtg aaatcaatcc ccgttgacgg gatggtttgc atgaaggtga aaatacctgg
     1021 caaaccaaag gagtaaactt tcgtttgcgc gtagccaatg atggtggccc ccaatgcccc
     1081 acttatgcag gcgataacga aggggtactt acgcggcagg ttgacgccat atactgccgg
     1141 ttcggtgata ccaaacagcc ccgtcaacgc cgccgatccc gctaccactt ttttctgcgc
     1201 atcgcgttcg cagaggaaga cgccgagcgc cgccccgact tgcgccataa tggcgggcat
     1261 taacagcggg atcatggtgt cgtagcccag cacggtgaag ttattgataa ttaaaggtat
     1321 caaaccccaa tgcagaccga acatcacaaa gatttgccag aagccgccca ttaccgcgcc
     1381 tgcaaatgca ggaaccgcct gataaagcca gagataaccg gcagcaatca gttcacttat
     1441 ccaggttgat agcggcccca ccagcagaaa tgtgatgggc gtgatgacca tcaaacatag
     1501 caagggtgtg aagaaatttt tgattgccga aggtaaccac gcattaagtc ggcgttccag
     1561 aatgctgcac aaccaggcag aaaaaataat gggaataacc gatgacgagt aattcaacaa
     1621 tgtgaccgga atacccagga aatccaggcc cagcgcatcc actttttgcc cgttctcgaa
     1681 ggcagtcaga attaatggat gcactaacgc tccgccaatc accatggcag taaatgaatt
     1741 gccgccgaag cgtttccctg cggtgtagcc caggattatc gggaagaacc aaaacaaggc
     1801 atcactggcg ctaaataaaa ttaaataagt accactttgt tcggtcgtcc actgaaaagt
     1861 gagtgccaga gccaacatac ctttcaggat cccggttgcc gccatcaaac cgatcagagg
     1921 cgtaaaaata cctgaaataa cataaacaaa gcggtttaac agattacctt tatcatcatt
     1981 ttccggtgcc tgttgcgctt tttcgccaag gcctgccacg ctgttaaccg ccaggaagac
     2041 atcggccaca tggttaccta tgacaacctg aaactggcca ccgctttcca ccaccataat
     2101 aataccgggg gtctttttca gtacctcagc ttgcgctttg ctttcatcct ttaacttaaa
     2161 acgtaatcgc gttgcacaat gcatcagact cacaatgtta tctgcgcccc cgactcctgc
     2221 gactattgtt ctggctaact ccgtcataac ttgccctcta ccgctttgcg gcaaaactcc
     2281 aaaaaaaaac ctgaaaaaaa cggcctgacg tgaatcaagc aatttttttc aggttttgcc
     2341 cgcttagtgc ggtaacaatc ctttattcag taataatatt tcagtgttct ttgcgcacgc
     2401 gctctatatt tatggctaaa aacataatct ctgcaggtga gattttacgt tgatactgca
     2461 aaccaataaa aatggcgatc cgctccgcac attgccatgc ttgcgggtaa ttttgcttta
     2521 ctgcttgttg taatgattca tcactatcgt taatcgatgc atgttcaata atacgccagg
     2581 ataaaaattt cagatgcgta accagtcgct gataactcaa gctttcttcc tggtaattaa
     2641 ggctgaactg aaattttatt aattgcagca tttctcgcat taattgcgtg acacctgcaa
     2701 catcctccat atttccgctc atttgggcac tgaccagatg catggcaata aagcccactt
     2761 catctttcgg taactgcacg cccaaccgtt tatcaataat ggttaacgct tcttccccta
     2821 gctggaactc tttcgggtaa agtcgctgga tatcccacag cagcgggttg ggcagtagca
     2881 cgttttgctg aaagcgttta atcgcaaact ggcaatggtc agttagcgag atataaatac
     2941 tgtcctgcaa ctttcccaga cgctcctgcg ccagagagat aatacgatca caggttgcca
     3001 tcacctcaag aggcatatga cttaagagtt cgcttaatcg cccgttcagt tcatgactgc
     3061 tcaaggcata ctctttttct attccacttg agttaattct ttcgcctggg cgtttttgaa
     3121 agccaattcc gcgccccatg acgacttttt cccgctgttg atcatcaata accaccacaa
     3181 cattattgtt gagaattttg gtgatttgca tgttcatagc aaggaccttt tttataaaca
     3241 aaaaaacccg acttcaccag tattctctgg ttatgtcagg ttttgcctgc gaatgcagta
     3301 acaatccagt cattaattaa tggtttttat aacgaacatc caggttcgaa aattaattta
     3361 gttgcgtgca gagaaaatag caatgctcta tttataaaag tatgaccatg ctcgcagtta
     3421 ttaattttgt gt
//
LOCUS       DAZFFE010000210         3283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZFFE010000210 DAZFFE010000000
VERSION     DAZFFE010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.212"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..675
                     /locus_tag="ACHJ69_004778"
     CDS             <1..675
                     /locus_tag="ACHJ69_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO5017569.1"
                     /translation="IERLVKDTCLNKKTVQAGLISLMKMGLISDTGERKGATKRVRVF
                     SLNITKNGNIKGNREGGNEPENGNVTENGNIPKNGMLNDPKNGIQNQSYNQSFNQERE
                     SRTKSGDSVPHDPGANNAVMNNFVSPGGPGQLGKFVMHEQWQPSDDFLRKSSLQGIYL
                     DSLPTAQELAEFRIYWMAEGKAYHQAQWEQKLARRLQISRQKQSTLPDNNVPHWNSPE
                     AWEDFL"
     gene            672..1457
                     /locus_tag="ACHJ69_004779"
     CDS             672..1457
                     /locus_tag="ACHJ69_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO5017570.1"
                     /translation="MNNVFTAIQNRDGEALSRMSGYEHQYVNNDNVVNMSAERLVDAL
                     FKQLKQLFPAAVVTNLKTPEQEVAAKQQWIAAFAEGGIRTREQVSAGMRHARASESPF
                     WPSPGQFIKWCKDSKMVLGVTIDDVMAEFHRYSKEKSLYPGGPERFPWRHPVMYWVVC
                     DTRRAMYQRQLSEIEVEKHARRLLDDWAKKVASGQQIPDPVISIQAKSEPMSTPPDTG
                     RDVYHPLGRSFGCMPNAATLGGITPAQWLMEEYRRGKEAGFIK"
     gene            1575..1919
                     /locus_tag="ACHJ69_004780"
     CDS             1575..1919
                     /locus_tag="ACHJ69_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFO5017571.1"
                     /translation="MRKQIQALGRLKTGQMNKTESAYCQHLELRKRAGEIVWYRFEGI
                     KLRLADNTFYTPDFAVMLATGEMELHEVKGFWTDDARVKTKVAADQYPFRIIGVTVKP
                     KKAGGGWKIEEF"
     gene            1981..2433
                     /locus_tag="ACHJ69_004781"
     CDS             1981..2433
                     /locus_tag="ACHJ69_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017572.1"
                     /translation="MEINIKGLVAAGHEMTSELKAECGAVDMRSVAKLISDLATQLEV
                     QLVRANALAAENAGLKSGAMDEIKVINRGGQAYCVKDGVRVNPMYARGWNDYRAKSLQ
                     SDTPATDAFLAEIRAPGVEMFADNLLCPDLDGTIRDFAAQLRKGGGQK"
     gene            2430..2747
                     /locus_tag="ACHJ69_004782"
     CDS             2430..2747
                     /locus_tag="ACHJ69_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017573.1"
                     /translation="MTITKQRVEEIISRIEMYGHGAGYTAEEVYDLAVLALNLSNIAN
                     LKRYELDMGGCDSCGQDCGADMTEDSDGDYVLFDDVVKLFEFDTTTQKLEGPAKETTS
                     ERN"
     gene            2734..>3283
                     /locus_tag="ACHJ69_004783"
     CDS             2734..>3283
                     /locus_tag="ACHJ69_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5017574.1"
                     /translation="MSEIDYQALREIAKQATQGEWVAFISSGTGTYAVHTPGDKRCED
                     VIKWTGFDGQNNAENNARYIAAFNPEVVQALLDERERNQQYIKRRDQENEDIALTVGK
                     LRVELEAAKSKLNEQREYYEGVISDGCKRIAELEAREVQLPTRYDLRYGHPINADERQ
                     VMIPKENGSWLYLIDLEHALRVA"
BASE COUNT          943 a          698 c          916 g          726 t
ORIGIN      
        1 atagaacgcc tcgttaaaga cacttgcctg aataaaaaaa ccgtgcaggc cgggcttata
       61 tcgctcatga aaatggggct tatttcagat accggagaga gaaagggagc gacgaaaaga
      121 gtgcgggttt tctctcttaa tataaccaaa aacgggaaca ttaaaggcaa ccgggaaggg
      181 ggtaatgaac ccgaaaacgg taatgttacc gaaaacggga atatacccaa aaacgggatg
      241 ttgaatgatc ccaaaaacgg gatccagaac cagtcatata accagtcatt taaccaagag
      301 agggagagca ggacaaaaag cggggattct gtgcctcatg accccggcgc aaacaacgcc
      361 gtgatgaata actttgtttc tcctggtggg ccagggcaat taggcaaatt tgtcatgcat
      421 gaacaatggc agccatcaga tgactttctt cggaaaagct cattgcaggg gatctacctg
      481 gacagtctgc caacggcaca ggaacttgca gagttcagaa tttactggat ggctgagggt
      541 aaggcatacc atcaggcaca gtgggagcag aagctggcaa ggcggctgca gattagcaga
      601 cagaagcaat caacattacc tgataacaac gttccgcact ggaacagccc tgaagcgtgg
      661 gaggatttct tgtgaacaac gtttttaccg cgatacaaaa ccgtgacgga gaagcccttt
      721 ctcgcatgtc aggttatgag catcagtacg tcaacaatga caatgtggtg aacatgtcag
      781 cagagaggct tgttgatgcc cttttcaaac agctgaaaca actgtttccg gcggcagtgg
      841 taaccaacct gaagacgcca gagcaggaag tagctgcaaa acagcagtgg attgctgcgt
      901 ttgccgaagg ggggatccga acccgtgaac aggtttctgc tggtatgcgc cacgcccgcg
      961 ccagtgaatc tccgttctgg ccgtcgcctg ggcaattcat caagtggtgt aaagacagca
     1021 agatggttct tggcgtcacc attgacgatg tgatggcgga gtttcaccgg tacagcaagg
     1081 aaaaaagttt atatcctggt ggtcccgaaa gattcccgtg gcggcatcct gttatgtact
     1141 gggtcgtatg tgatacccgc cgtgcaatgt atcagcgcca gcttagcgag attgaggttg
     1201 agaaacacgc gcgcaggctg ctcgatgatt gggcgaaaaa ggtggcttcc ggacagcaga
     1261 tacccgatcc ggtgatcagc atacaggcaa agtcagagcc catgagtaca cctccggaca
     1321 cagggagaga cgtttaccat ccactagggc gaagtttcgg gtgcatgcct aacgccgcca
     1381 cccttggggg aataacaccg gcgcagtggc tgatggagga atacaggcgg ggaaaggagg
     1441 caggatttat caagtaatac cagcgcgata gcgcattttt ttacgcctta gcaattacct
     1501 gttgggtaat aaaatattct aaattctatt gatttcgtgt cttatgtggt ttttaattac
     1561 ctcaggggta aatcatgaga aaacagatac aggctcttgg tcgactcaaa acaggccaga
     1621 tgaacaaaac agaatctgcg tattgccagc accttgagct gcgtaaacgt gcaggagaaa
     1681 tcgtctggta tcgatttgag ggtatcaagc tgcggttagc tgacaacacg ttctatacgc
     1741 ccgattttgc tgtgatgctc gccactggcg agatggaact gcacgaagtg aaaggtttct
     1801 ggaccgacga cgccagggtg aaaaccaaag tcgccgcaga tcagtatccg ttccgaatca
     1861 tcggggtaac ggttaaacca aagaaagcag gtggtggctg gaaaatcgaa gagttctgaa
     1921 tcgacgatct ttttagttat caatgtaatc aataagttat gtggataagc gagggtaaag
     1981 atggaaatta atatcaaagg gttagttgcc gccgggcatg agatgacttc ggaactgaaa
     2041 gctgaatgtg gtgccgttga tatgcgcagt gtggcaaagc tgatcagcga tttggcaacg
     2101 caactggaag tgcaactggt gcgtgctaat gcgctggctg ctgagaatgc ggggctgaag
     2161 tctggcgcta tggacgaaat caaggttatc aaccgtggag gtcaggcata ttgcgtaaaa
     2221 gatggagtgc gagttaatcc catgtatgca agagggtgga atgactatcg cgcaaagtct
     2281 ctgcaatcag acaccccagc caccgatgct ttcctggctg aaattcgtgc accgggggtt
     2341 gagatgtttg ctgacaatct gttgtgtcca gaccttgatg gcactatccg tgactttgct
     2401 gcccagcttc gcaaaggagg agggcaaaaa tgacaatcac aaaacaacga gtagaagaaa
     2461 tcatatcgcg cattgaaatg tatgggcatg gtgcagggta taccgccgaa gaggtttatg
     2521 accttgctgt actggcgctg aatttatcaa atatcgcaaa cctgaagcga tacgagcttg
     2581 atatgggagg ttgcgactcg tgcggtcagg attgtggcgc tgacatgact gaagattctg
     2641 atggcgatta tgtcctgttt gatgacgtgg ttaagttgtt tgagtttgat acaaccactc
     2701 agaagttaga aggcccagca aaggagacaa ccagtgagcg aaattgacta tcaggcactg
     2761 cgtgagatag caaaacaggc cacgcagggc gaatgggtcg catttatttc gtcgggtact
     2821 ggtacatatg cggtgcatac acctggtgat aaacgatgtg aagacgttat caaatggacc
     2881 ggctttgatg gacagaacaa cgcagagaac aacgctcgtt atatcgcagc tttcaaccct
     2941 gaagtagtgc aggcgctgct ggatgaacgg gaaagaaacc agcaatacat caaacgccgt
     3001 gaccaggaga acgaggatat tgcgcttacg gttgggaagc tgcgcgttga gcttgaagca
     3061 gcaaaatcaa aactcaacga gcagcgcgag tattacgagg gagttatctc tgatgggtgc
     3121 aagcgtattg ctgaactgga agcgcgggaa gttcaattac cgactcgcta cgaccttcga
     3181 tatggacacc cgataaatgc agatgagcga caagtcatga tacctaaaga aaatggcagt
     3241 tggctttacc tgattgacct agaacacgca ttacgcgtcg ctg
//
LOCUS       DAZFFE010000211         3228 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZFFE010000211 DAZFFE010000000
VERSION     DAZFFE010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.213"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1002
                     /locus_tag="ACHJ69_004784"
     CDS             <1..1002
                     /locus_tag="ACHJ69_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO5017575.1"
                     /translation="LGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKITGKTPVAAY
                     VSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLNDPAYRRLRI
                     ITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFDTVEVDFGRSEGNNIEQADGKK
                     WSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKLDTRRGSNSQ
                     LETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLGNTAAEGIRC
                     YGAIQDAQALSEGVVVSSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK"
     gene            1054..1437
                     /gene="gpFI"
                     /locus_tag="ACHJ69_004785"
     CDS             1054..1437
                     /gene="gpFI"
                     /locus_tag="ACHJ69_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO5017576.1"
                     /translation="MATKEENLNRLRELSGLLGREADMSGSAADIAQRVSEWEEELAV
                     SPEGIMHSDESGADQNHTDDGEQLNNTDAPDDVKAVRVRKCLQVMGYCPETGRPVELA
                     LRGMRVLVPSSLATAMIQHGTAEYA"
     gene            1430..1783
                     /locus_tag="ACHJ69_004786"
     CDS             1430..1783
                     /locus_tag="ACHJ69_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO5017577.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGIRAQFTSGAQRGGEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEMFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            1799..2374
                     /locus_tag="ACHJ69_004787"
     CDS             1799..2374
                     /locus_tag="ACHJ69_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5017578.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMTINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNAKIIVNRGDLPVIKLGIRMLGRRPDSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPQARYEEGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVDRIRRERLPKELAYALKQQLRIAIKR"
     gene            2371..2766
                     /locus_tag="ACHJ69_004788"
     CDS             2371..2766
                     /locus_tag="ACHJ69_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO5017579.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            2774..>3228
                     /locus_tag="ACHJ69_004789"
     CDS             2774..>3228
                     /locus_tag="ACHJ69_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO5017580.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKADAYVNPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEKGQKGLIAWFES
                     GDVRAYKIRFPNGTVDVFSGWVSSIGKAVTAKEVITRTVKVTNVGRPSMA"
BASE COUNT          774 a          725 c         1006 g          723 t
ORIGIN      
        1 ctcggttata ccgaacaaaa agttaaattt cgtgcgctgt ttctggaact atttttccgc
       61 cgtacggtga atttccacac cgaagaggtg atgctggaca aaattaccgg aaaaacgccg
      121 gtggcggcct atgtctcccc ggtcgttgaa ggtaaagtgc ttcgtcatcg tggtggtgaa
      181 acccgcgtgt tgcgtccggg ctacgtcaag ccgaaacacg aatttaatta ccagcaggcg
      241 gttgagcgcc ttcccggtga agatccggct cagctgaacg acccggccta ccgtcgtctg
      301 cgtatcatca ctgataacct caaacaggaa gagcacgcta ttgtgcaggt ggaagaaatg
      361 caggcggtga atgccgtgct gtatggcaaa tacaccatgg aaggggaaca gtttgacacg
      421 gttgaggtgg attttggacg ctctgaagga aataacattg agcaggccga cggtaaaaaa
      481 tggtctgagc aggaccgtga tacgtttgat ccgacgcatg atattgacct ttactgcgat
      541 caggccagcg gtcttgtgaa tattgccatt atggacggta ctgtctggcg tctgctgaat
      601 ggctttaagc ttttccgcga aaaactggat acccgtcgcg gctcaaattc acaactcgaa
      661 acggcagtga aagacctggg ggcggtggtg tctttcaaag ggtattacgg tgatctggcc
      721 attgtggtgg cgaaaacgtc ttatgtggca gaagacggta ccgaaaaacg ttatctgccg
      781 gagggtacac tggtcctggg gaatacggca gcagagggca ttcgttgcta tggtgccatt
      841 caggatgcgc aggcgttgtc cgaaggtgtg gtggtctctt cccgttatcc gaaacactgg
      901 ctgacggtgg gggatcctgc ccgtgaattt accatgacgc agtccgcacc gctgatggtg
      961 ttgccggacc cggatgagtt tgtggtggtg caggtgaaat aatccgtgag cgggggcgaa
     1021 atgcccccgt gtcttttttc acaggagacc gagatggcaa caaaagaaga aaatctgaat
     1081 cgccttcgtg aactgtctgg cctgctgggg cgagaggcgg atatgtcggg gagtgctgcg
     1141 gatattgctc aacgtgtgtc tgagtgggaa gaggagcttg ctgtttcccc ggagggcatt
     1201 atgcactctg atgagagcgg ggctgatcaa aatcacacag acgatggtga gcagttgaac
     1261 aacacggatg ctccggatga tgttaaagcc gtccgggtac ggaagtgcct gcaagtaatg
     1321 gggtattgcc cggagacagg tcgtcccgtt gagctggcgt tacggggtat gcgtgttctg
     1381 gtgccatcat cactggcaac ggccatgata cagcacggaa cggctgaata tgcgtgattt
     1441 tcagaatgcc tttgatgctg ccctcgccgg ggtggacagt acgatcgttg aagtgatggg
     1501 gatccgtgcg cagttcacct ccggagcaca acgtggcggc gaagttcagg gggtttttga
     1561 cgatccggag tcgctgggtt ttgccggtgg cggggtccgt attgaaggaa gcagcccgtc
     1621 attatttgtg cggacggata cggtgcgtgc cgtgcggcgt ggtgacacgc tgaccattaa
     1681 cggcgagatg ttctgggtgg atcgtgtttc tccggatgac gggggaagct gttatctctg
     1741 gctcaaccgt gggcaaccac ccgctgttaa ccggcgacga taaacgcagg gtgaaattat
     1801 ggcgataaaa gggcttgatc aggcgattga taatctgagc cgggttcgta aaaacgccat
     1861 tccggcggct tcagcaatga cgattaaccg cgtggccaca acggcgatta atcagtcttc
     1921 atcacaggtt gcccgggaga caaaggtacg ccggaaactg gttaaggaac ggtccagact
     1981 gaaacgggcc acggtcagaa atccgaatgc aaaaattatc gttaaccgcg gtgatctccc
     2041 tgtgattaag ctggggatca gaatgctggg gcgtcgtccg gacagcatac tcaaagccgg
     2101 tcagcatcgt tatcagcggg catttatcca gcgattaaat aatgggcgct ggcatgttat
     2161 gcaacgtctt cctcaggcca gatatgagga gggcaatgac gacaagggaa ggaaaaagcg
     2221 taatcgcctt cccattcagg tggtgaaaat cccgatggcg gccccactga aacaggcatt
     2281 tgatgagaat gttgaccgta tccggcgtga acgcctgcct aaagaactgg catacgcgct
     2341 gaaacaacaa ctgaggattg cgataaaacg atgaaacaca ctgacattcg tgccgcagtg
     2401 ctggatgcac tcgagcagca tgaacacggg gcgacgctgt ttgatggtcg ccccgttgtt
     2461 tttgacgaag aggattttcc tgcgatcgcg gtttatctga cggatgcaga gtataccggt
     2521 gaagagctgg atgcagatac ctggcgggcc acactgcata ttgaggtgtt tttaccggca
     2581 caggtacctg attcggagct cgatcagtgg atggaaagcc ggatttatcc ggcgatgact
     2641 gcgatccctg cactggcagg actgattacc acgatggtta cgcagggcta tgagtatcgt
     2701 cgtgatgacg atatggcgtt atggagctct gcggatctga cttattccat tacatacgag
     2761 atgtgaggac gatatggcaa caccaaatcc cctggagccg gtaaaaggtg ccggtaccac
     2821 tctgtgggtt tacaacggca aggctgatgc ttatgtaaac ccgttgtcag acgatgactg
     2881 gcagcgactg gctaaggtga aggatctgac gccgggcgag atgacggctg aatcctacga
     2941 tgataactac ctggatgatg aagacgcgga ctggaccgcg accgggcagg gacagaaatc
     3001 tgcaggtgat accagtttta cgctggcctg gaaaccgggt gagaaaggtc agaaagggct
     3061 tatcgcatgg tttgaaagcg gggatgtcag ggcctataaa atccggtttc cgaatggtac
     3121 ggtggatgtg ttcagtggct gggtcagcag tatcggtaag gccgtgacgg cgaaggaagt
     3181 gatcaccaga acggtgaaag ttactaatgt gggacgcccg tcgatggc
//
LOCUS       DAZFFE010000212         3226 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFFE010000212 DAZFFE010000000
VERSION     DAZFFE010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.214"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(90..617)
                     /gene="mobB"
                     /locus_tag="ACHJ69_004790"
     CDS             complement(90..617)
                     /gene="mobB"
                     /locus_tag="ACHJ69_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO5017581.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQDG"
     gene            complement(599..1183)
                     /gene="mobA"
                     /locus_tag="ACHJ69_004791"
     CDS             complement(599..1183)
                     /gene="mobA"
                     /locus_tag="ACHJ69_004791"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO5017582.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQVSGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLTHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDRKEAFINVNTPEELARWQEKR"
     gene            1253..1522
                     /locus_tag="ACHJ69_004792"
     CDS             1253..1522
                     /locus_tag="ACHJ69_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO5017583.1"
                     /translation="MKCKRLNEVIELLQPAWQKESDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1599..2585
                     /gene="srkA"
                     /locus_tag="ACHJ69_004793"
     CDS             1599..2585
                     /gene="srkA"
                     /locus_tag="ACHJ69_004793"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO5017584.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQLALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2602..>3226
                     /gene="dsbA"
                     /locus_tag="ACHJ69_004794"
     CDS             2602..>3226
                     /gene="dsbA"
                     /locus_tag="ACHJ69_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO5017585.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKELTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          816 a          783 c          788 g          839 t
ORIGIN      
        1 gaaggccatc cgtcaggatg gcctttttgc attggcgcag aaaaaatgcc ggatgcgacg
       61 ctggcgcgtt tgctccagcc tactcttgct tatccgtcct gcttttgcat ccactccacc
      121 acaaaatcag ccagcccctc aacatcatta atatccagta acgcgacatc aagattaagc
      181 ggcacatcac tggctacagc aataacatgc ctgtctatca ctaattcttc cggtcgatgt
      241 ccggctccat cgcgaaacag cacaatcttt gcgatctctt catgcttaaa cccttcgacc
      301 agaatcaaat ccagctttga ggtatccatt cgacttgcga gaaaatgtag atccagctct
      361 tcttcgtctg gtgtttccgt catcaaggcc catcgctgtt ggctggcaac gatggtttgc
      421 gccgcgccag ccttgcgcaa ctcatagcta tctttgcccg gtttatcaac atccatatca
      481 tggtgcgtat gcttaatcag ccctggacgg atccctctgg cgcataatgc cgggatcaat
      541 tttttcagca gcgttgtttt tccggtgcca ctccatgcag caaaggcgag taacggtatc
      601 atcgtttttc ctgccatcgg gcaagctcct ctggcgtatt cacgtttata aatgcctctt
      661 tacgatcgct gaaatcaacc gcatgaccgc cagccagacg cataaatgcc ataacccggc
      721 gttctcctgc ttgcagatat tccagtaata aaggctcaat tgcgcggttt accagagcaa
      781 tagtcgggtg atcgcgttca ccgtcgtgga cccacacgac aggcgcatct ttgcgctgat
      841 gagtaagccg ggcggctaaa tcatggggaa tgtaaggcgt atcgcacggg caaaacaaaa
      901 accactcacc cgcttcctgc tgcattactg aaagcattcc tgccagaggg ccagggtaat
      961 ccgccagtga atcttcaatc actttcagac cgcttacctg atagatttcc tgatgacgat
     1021 tagcattaac cacgacgtga gaaagctgcg tcataagcgc gtcagcgaca tgctgccata
     1081 atggtttgcc gtttaattca agcaatcctt tatctacgcc gcccattcgt ctggctttac
     1141 cgcccgccag cacaacgcct gttatcgtcg tcatcagatt caccgatatc gcctctttta
     1201 ttgtgggatt gaccctgcta acgtgtctgt ctcaagagta aggagcatta ctatgaaatg
     1261 taaacgtctg aatgaagtta ttgaactcct ccagccagcc tggcaaaaag agtcagacct
     1321 taacctgctg caatttttgc agaaactggc gaaagagtca ggttttgacg gcgaactggc
     1381 ggatttgacg gatgacattc tgatctatca cctgaaaatg cgtgattccg cgaaagatgc
     1441 ggtgatcccg ggtttgcaga aagattatga agaagatttc aaaacggcac tattacgcgc
     1501 acgtggcgta attaaagagt aaaagcttgt aagcggcgca accaaaatca tcgtgaaatg
     1561 atatccttca tcattcgtaa tgttttccgg atgatgggat gaacaacagc gcttttactt
     1621 tccagacact acacccggat accatcatgg acgctctgtt tgagcatggg attcgggtgg
     1681 attccggtct taccccgctt aacagctatg aaaaccgtgt ctatcaattt caggacgaag
     1741 atcgtcgacg ttttgtcgtc aaattttatc gccctgaacg ttggacagcc gatcaaatcc
     1801 tcgaagaaca tcaacttgcg ttgcagctgg ttaacgatga agttccggtc gcagcacctg
     1861 tggcctttaa tggtcagact ttattgaacc atcagggatt ttatttcgct gtttttccaa
     1921 gcgtcggtgg tcgccagttc gaagctgata atatcgatca gatggaagcg gttgggcgtt
     1981 atttagggcg tatgcaccag acggggcgca aacagctttt tatccatcgc ccgaccatcg
     2041 gtttgaatga atatctcatt gagccacgca agctgtttga ggacgctaca ctaatacctt
     2101 ccgggttgaa agcggcattc ctgaaagcga cagatgagct gattgccgcc gttacagcac
     2161 actggcggga agatttcacc gttctgcgac tacatggaga ctgccacgcc gggaatattc
     2221 tctggcgcga tggtccaatg tttgttgatc tggatgatgc acgtaatggt ccagccgttc
     2281 aggatttgtg gatgttgctc aatggtgata aagccgagca gcggatgcaa ctggaaacta
     2341 ttattgaggc ttatgaagaa tttagcgagt tcgacaccgc tgaaatcgga ctgattgaac
     2401 ctttacgcgc catgcgtttg gtttattatc ttgcctggct aatgcggcgt tgggctgatc
     2461 ccgcgttccc gaaaaatttc ccgtggttaa ccggggaaga ttactggcta cgacagacgg
     2521 cgacttttat agaacaggca aaagttctac aagaaccccc tttgcaatta acacctatgt
     2581 attaatcgga gagagtagat catgaaaaag atttggctgg cgctggctgg tttagtttta
     2641 gcgtttagcg catcggcggc gcagtatgaa gatggtaaac agtacactac cctggaaaaa
     2701 ccagttgctg gcgcgccgca agtgctggag tttttctctt tcttctgccc gcactgctat
     2761 cagtttgaag aagttctgca tatttctgat aacgtgaaga aaaaactgcc ggaaggcgtg
     2821 aagatgacta aataccacgt caacttcatg gggggtgacc tgggcaaaga gctgactcag
     2881 gcatgggctg tggcgatggc gctgggcgtg gaagacaaag tcacagttcc gctgtttgaa
     2941 ggcgtacaaa aaacccagac cattcgttca gcatctgata tccgcgatgt atttatcaac
     3001 gcaggtatta aaggtgaaga gtacgacgcg gcgtggaaca gcttcgtggt gaaatctctg
     3061 gtcgctcagc aggaaaaagc tgcagctgac gtgcaattgc gtggtgttcc ggcgatgttt
     3121 gttaacggta aatatcagct gaatccgcag ggtatggata ccagcaatat ggatgttttt
     3181 gttcagcagt atgctgatac tgtgaaatat ctgtccgaga aaaaat
//
LOCUS       DAZFFE010000213         3208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZFFE010000213 DAZFFE010000000
VERSION     DAZFFE010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.215"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            166..1848
                     /gene="ydeN"
                     /locus_tag="ACHJ69_004795"
     CDS             166..1848
                     /gene="ydeN"
                     /locus_tag="ACHJ69_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5017586.1"
                     /translation="MKSALKKSIVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQNKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDNSQPPLSEVNQEKFNNIKKALSKAK"
     gene            1900..3057
                     /gene="ydeM"
                     /locus_tag="ACHJ69_004796"
     CDS             1900..3057
                     /gene="ydeM"
                     /locus_tag="ACHJ69_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO5017587.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQIEFNTLTVI
                     NNINVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMRWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCVYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3023..3148)
                     /locus_tag="ACHJ69_004797"
     CDS             complement(3023..3148)
                     /locus_tag="ACHJ69_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017588.1"
                     /translation="MKTKHPTGIYVGCLVKVIRRRLRMELRGSVINYSPFVLQHP"
BASE COUNT          979 a          688 c          644 g          897 t
ORIGIN      
        1 aaattactta gtagaacaga aagtaatgtc aacagtttat ggactgttat ttctcttttt
       61 taagctaaat ctgctatcta tttatgtgac taacttcact tacattaact tatttctctt
      121 cgtaaaatta ctttggaatt aagtacaata agaagaggaa catttatgaa gtctgcatta
      181 aagaaaagta tcgtaagtac ctcgatatct ttgatactgg catctggtat ggctgcattt
      241 gctgctcatg cggcagatga tgtaaagctg aaagcaacca aaacaaacgt tgctttctca
      301 gactttacgc cgactgaata cagtaccaaa ggaaagccaa atattatcgt actgaccatg
      361 gatgatcttg gttatggaca acttcctttt gataagggat cttttgaccc aaaaacaatg
      421 gaaaatcgtg aagttgtcga tacctacaaa atagggatag ataaagccat tgaagctgcg
      481 caaaaatcaa cgccgacgct cctttcatta atggatgaag gcgtacgttt tactaacggc
      541 tatgtggcgc acggtgtttc cggcccgtcc cgcgccgcaa taatgaccgg tcgagcgccc
      601 gcccgctttg gtgtctattc caatactgat gctcaggatg gtattccgct aacagaaact
      661 ttcttgcctg aattattcca gaatcatggt tattacactg cagcagtagg taaatggcac
      721 ttgtcaaaaa tcagtaatgt gccagtgccg gaagataaac aaacgcgtga ctatcatgac
      781 aacttcacta ccttttctgc ggaagaatgg caaccgcaaa accgtggctt tgattacttt
      841 atgggattcc atgctgcagg aacggcatat tacaactccc cttcactgtt caaaaaccgt
      901 gaacgcgtcc ccgcaaaagg ttatatcagc gatcagttaa ccgatgaggc aattggcgtt
      961 gttgatcgtg ccaaaacact tgaccagcct tttatgcttt acctggctta taatgctccg
     1021 catctgccaa atgataatcc tgctccggag caatatcaga agcaatttaa taccggtagt
     1081 caaacagcag ataattacta cgcttccgtt tattctgttg atcagggtgt aaaacgcatt
     1141 ctcgaacaac tgaagaaaaa cggacagtat gacaatacaa ttattctctt tacctccgat
     1201 aatggtgcag ttatcgatgg ccctctgccg ctgaacgggg cgcaaaaagg ctataagagt
     1261 cagacctatc ctggcggtac tcacacccca atgtttatgt ggtggaaagg aaaacttcaa
     1321 cccggtaatt atgacaagct gatttccgca atggatttct acccgacagc tcttgatgca
     1381 gccgatatca gcattccaaa agaccttaag ctggatggcg tttccttgct gccctggttg
     1441 caaaataaga aacagggcga gccacataaa aatctgacct ggataacatc ttattctcac
     1501 tggtttgatg aggaaaacat tccattctgg gataattacc acaaatttgt ccgccatcag
     1561 tcagacgatt acccgcataa ccccaacacc gaggacttaa gccaattctc ttatacggtg
     1621 agaaataacg attattcgct cgtctataca gtagaaaata atcagttagg tctatacaaa
     1681 ctgacggatc tacagcaaaa agataacctt gccgccgcca atccgcaggt cgttaaagag
     1741 atgcaaggcg tggtaagaga gtttatcgat aacagccagc caccgcttag cgaggtaaat
     1801 caggagaagt ttaacaatat caagaaagca ttaagcaaag cgaaataact aaaccttcat
     1861 gcggcggatt tttccgccgc cttattgagc gagatagcga tgcacgttac agccaagccc
     1921 tccagttttc aatgtaatct caaatgtgat tactgttttt accttgaaaa agagtcgcag
     1981 tttactcatg aaaaatggat ggatgacagc actctgaaag agttcatcaa acaatatatc
     2041 gcagcgtctg gcaatcaggt ctattttacc tggcaaggcg gtgaacccac tctggctggc
     2101 ctggattttt tccgtaaagt tattcactat caacaacgct atgcaggcca aaaacgaatt
     2161 tttaatgcat tgcaaacgaa tggcatttta ttgaataatg aatggtgtgc ctttctcaaa
     2221 gaacatgagt ttctggttgg tatctcgatc gatggacccc aagaactaca tgaccgctac
     2281 agacgcagta attcaggtaa cggtactttt gcaaaagtga tagcagccat cgagcgtctg
     2341 aaatcatatc aaatagagtt taatacgtta actgtcatta ataacattaa tgtccattac
     2401 cctcttgagg tttatcattt tttaaaatct atcggcagta aacatatgca atttatcgaa
     2461 ttgctcgaaa ccgggacgcc gaatattgat ttcagtggtc atagtgagaa cacattccgt
     2521 atcattgatt tttctgtgcc tcccacggct tatggcaagt ttatgtcaac catttttatg
     2581 cgatgggtta aaaacgatgt gggtgaaatt ttcatccgtc agtttgaaag ctttgtcagc
     2641 cgttttttgg ggaatgggca taccagttgt attttccagg agtcctgcaa ggataatctg
     2701 gttgttgaaa gtaatggaga catttacgaa tgcgaccatt ttgtctatcc acagtacaaa
     2761 attggaaaca ttaataaatc ggaactcaaa acgatgaaca gtgtacaact gacagcgcaa
     2821 aaaaaacgga tttcagccaa atgtcagcaa tgtgtatata aacctatctg caatggtggt
     2881 tgccctaagc atcgtattac taaagtaaac aatgagactg tttcctattt ttgcgaaggt
     2941 tataaaatcc ttttttcaac catggtacct tatatgaacg ccatggtgga gttagctaag
     3001 aacagagtac cgctttacca cattatggat gttgcaaaac aaatggagaa taattaataa
     3061 cgctccccct tagctccatt cttagcctcc ttcttatgac cttaacaagg cacccgacat
     3121 aaattcctgt cgggtgtttc gtcttcatca tcttcctttg cgaaaatcct ggcttacgag
     3181 gtatgcgtca gggcattagc aataccct
//
LOCUS       DAZFFE010000214         3205 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZFFE010000214 DAZFFE010000000
VERSION     DAZFFE010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.216"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            62..2578
                     /locus_tag="ACHJ69_004798"
     CDS             62..2578
                     /locus_tag="ACHJ69_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017589.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFVLLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVALLWAASQIQALKRLTSAIRQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTECIWLTLISQRISRGELREFELADGNWLLDNAWYERNMAGFNEKLRES
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEAFPPELPA"
     gene            2654..3109
                     /locus_tag="ACHJ69_004799"
     CDS             2654..3109
                     /locus_tag="ACHJ69_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO5017590.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHHVSLPLSVFDPQDYHPLLITVSGKNVN"
BASE COUNT          787 a          798 c          844 g          776 t
ORIGIN      
        1 aacgtttcag tgccacattc actggcattc acaataacat gatattcatc acggagtgac
       61 tatgttacag atagtcggcg cgctgattct gctgatcgca ggatttgcca ttcttcgcct
      121 tttgttcaga gcattaacca gcacagcgtc tgcgctggca gggttcgtat tgctgtgtct
      181 gttcggcccg gctttactgg ctggctatat cactgaacgc ataacccggt tattccatat
      241 tcgctggctg gcaggcgtat ttctgacgat tgccggaatg attatcagct tcatgtgggg
      301 acttgatggt aaacatatcg cactggaggc tcatactttt gactctgtaa aatttattct
      361 gaccaccgct ctcgccgctg gtctgctggc tcttcccgtg cagataagaa ccattcagca
      421 gaacgggctc acacctgaag atatcagcaa ggaaattaac gggtattact gctgttttta
      481 tactgctttt ttccttatgg cgtgttctgc atacgcacca ttgatcgcat tgcagttcga
      541 tatttcaccc tcactgatgt ggtggggcgg gttgttgtac tggctggctg cattagtggc
      601 gctgctatgg gcggccagcc agatccaggc gctgaaaaga ctgaccagtg ccatccgcca
      661 gacactggaa gaacaaccgg tgctcaacag taaatcgtgg ctgagcagtt tgcaaaacga
      721 ttacagcctt cctgaaacgc tgacggagtg catctggctg acactcattt cacaacggat
      781 ttcccgggga gaactgaggg aatttgaact ggcagacgga aactggctac tggacaatgc
      841 ctggtatgaa agaaacatgg cgggtttcaa cgaaaagctg agagagagcc tgtcatttac
      901 ccctgatgaa ctgaaaaccc tcttccggaa ccgcctgaat ttatcaccgg aagcgaatga
      961 cgattttctc gatcgttgcc tggacggcgg tgactggtac cccttttcag aaggccgccg
     1021 ttttgtatca ttccaccacg tggatgagct tcgtatctgt gcctcctgcg ggctgacaga
     1081 agtacatcat gccccggaaa atcataagcc ggatccggaa tggtactgct cctctctttg
     1141 tcgcgaaaca gaaacactgt gtcaggacat ttatgaacgt tcttacaccg gttttatttc
     1201 cgatgcaacg gcgaatggtc tgattctcat gaaactgccg gaaacctgga gtacaaatga
     1261 gaaaatgttt gcttccggag ggcagggaca tgggtttgcc gctgaacggg gaaaccatat
     1321 tgtcgacaga gtccgtctga aaaacgcacg gatcctcggt gataataatg ccagaaatgg
     1381 agcagacaga ctggtcagcg gaacagaaat ccagacgaaa tattgttcaa ctgcagcccg
     1441 tagcgtcggt gcggcattcg acggacaaaa cggacagtat cgttacatgg gaaatcatgg
     1501 tcccatgcaa ctggaagtcc cccgtgatca gtatgccggc gctgtggaaa ccatgaagaa
     1561 taagatccgc gaaggtaaag tacccggtgt aaccgatccc gcagaagcgt cccggctgat
     1621 tcgtcgggga catctgactt atacccaggc ccgtaatatc acccggttcg ggaccatcga
     1681 atcggtcact tatgatattg ccgaggggtc ggttgtcagt ctggcggccg gagggatcag
     1741 ttttgccctg acggcatcgg tcttctggct cagcaccggc gatcgcgatg ctgccctgca
     1801 gacagctgct gtccaggcag gaaaaacctt cacccgcaca ctggctgtct acgtcacaac
     1861 ccagcaactt caccggctca gtgttgttca gggtatgctg aagcatattg atttttcgac
     1921 ggccagcccg actgtccggc aggcgcttca gaaggggacc ggtgcaggaa atatcagtgc
     1981 cctgaacaaa gtgatgaagg gtacgctggt gacatctctg gcactggtag ctgtcacaac
     2041 cggccctgac atgatcaaaa tgttgcgggg acggatctcc ggtgcgcagt tcatcaggaa
     2101 tcttgccgtg gcatcttccg gtgtggcagg tggtgctgtc gggtcagtgg cgggcgggat
     2161 attgttcagt ccactgggac catttggtgc actgacaggg cgtgtggttg gcggtgttct
     2221 ggggggaatg attgcctccg ctgtatcagg aaaaattgcc ggagcgctgg ttgaagaaga
     2281 tcgcgtcaaa attctggcaa tgattcagga gcaggtgaca tggcttgccg gcagtttcct
     2341 gctgaccgga catgagattg aaaatctgaa cgcgaatctg gcccgtgtta tcgatcagaa
     2401 tgctctggag atcattttcg ccgccggtat acaacaacgg gccgcgacca atatgttaat
     2461 caaaccactg gtggtcagta tcatcaggca acgccccgtc atggaatatg atgcatccca
     2521 tctcggcaat atggttaacc gactggaaga agcattcccc ccggagttac cggcataacg
     2581 gctattctgc gccttttacg ttttatcacc tgatttgtca gtgtcatcgt catgactcag
     2641 agaggtaaat accatgattc acctgttcaa aacctgcatg attaccgcct tcattctggg
     2701 gttaacgtgg tctgccccac tccgggcaca ggatcaacgt tacatcagta tacgcaatac
     2761 agatacgata tggctcccgg gaaatatttg tgcctaccag ttccggctgg ataatggcgg
     2821 aaacgatgaa ggatttggcc ccctcaccat cactctgcaa ctcaaagaca aatatggtca
     2881 gacgctggtg accagaaaaa tggaaacgga agcctttggt gacagtaatg ccacgcgaac
     2941 cacagacgca tttctggaaa cggagtgcgt ggaaaatgtc gccacaaccg aaatcattaa
     3001 agcaactgaa gaaagtaacg gccatcatgt cagtctgccg ttatcggttt tcgatcccca
     3061 ggactaccat ccactgctga ttaccgtttc cggaaaaaac gttaactgat ccctgaagtc
     3121 agtttcatgc ttcagcctct gtcacaacgc gtggcagagg ctttttattt atcaggagaa
     3181 aacagatatg ccgggttaca cagag
//
LOCUS       DAZFFE010000215         3178 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFFE010000215 DAZFFE010000000
VERSION     DAZFFE010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.217"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            66..446
                     /locus_tag="ACHJ69_004800"
     CDS             66..446
                     /locus_tag="ACHJ69_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5017591.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVR
                     QQRLKLSLSGRLVIRIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     GQPVSGGRLGVKIYPIMLEGQVKK"
     gene            443..1263
                     /locus_tag="ACHJ69_004801"
                     /pseudo
     CDS             443..1263
                     /locus_tag="ACHJ69_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
     gene            1490..1687
                     /locus_tag="ACHJ69_004802"
     CDS             1490..1687
                     /locus_tag="ACHJ69_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO5017592.1"
                     /translation="MLKQQDMTETARVVFNELSATEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            1838..2896
                     /locus_tag="ACHJ69_004803"
     CDS             1838..2896
                     /locus_tag="ACHJ69_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO5017593.1"
                     /translation="MLSTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSASQWQLPDESDYLKLQALFARVA
                     EDKHQRGELEKPHHQLVSTYSELNRQYTELQSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAIALGRRATGVELET
                     ERFEQTVREVQNLVSQNG"
     gene            2937..3012
                     /locus_tag="ACHJ69_004804"
     tRNA            2937..3012
                     /locus_tag="ACHJ69_004804"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2970..2972,aa:Ile,seq:cat)
     gene            3022..3098
                     /locus_tag="ACHJ69_004805"
     tRNA            3022..3098
                     /locus_tag="ACHJ69_004805"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3056..3058,aa:Arg,seq:tcg)
BASE COUNT          818 a          691 c          911 g          758 t
ORIGIN      
        1 tgttgttgct gattcgtttt ttaatgcacg cgagaaattc ggatgttctg aagtggaaag
       61 cataaatgac cgaacgcata gaatttgttt tgccttaccc gccgacggtg aacacttact
      121 ggcgacgccg tggcagcaca tattttatct cggaggaggg aaagcgttat cgccgggctg
      181 tggcgcttat tgttcgccag cagcggctga aattaagcct gtccggaagg ctggtaatca
      241 ggattattgc agagccaccg gataagcgtc gtcgtgacct ggacaatatt ctgaaagcac
      301 cgctggatgc gctgacgcac gcggggttgt taatggacga tgagcagttt gatgaaatca
      361 atattgtacg tggccagcca gtatctggtg gacgtctggg ggtgaagatt taccccataa
      421 tgcttgaagg gcaggtcaaa aaatgaaact ggaagattta ccgaaatact actccccaaa
      481 atcccccggc ctgactgatg catcggcctc aacgtcgaaa gatgcgctga gtatcactga
      541 tgtgatggcc gcgcagggca tgacacagaa tcgggctgag atggggtttt ctgcgttcct
      601 tgggaaaatg ggcattagta tgaatgacag agagcaggca acagaattgc tgacagaata
      661 tgcactcagt cggtgtgatc gcgtggcggc gttaagaaaa ctcccggcag aaataaaacc
      721 ggcagtgatg cgtattatgg cttcgtatgc gtttgaagat tatgcccgta gcgcggcgag
      781 caaaaaacag tgcccctgct gtcacggaaa aaaatttatt gaaagcgagg tttttacaaa
      841 caagatccag tatccggatg gtaagccgcc agtgtgggca aagtgcacaa aaggcgtgta
      901 tccgtcttac tgggaggaat ggaaaaaagt cagggaggtg gtaaaagttg cctgtccgga
      961 gtgtggaggg aagggagagg tatccactgc ctgtaaagat tgtcgtgggc gcggtgttgc
     1021 cattcatcgt gaagagtcgg aaaaacaggg tgtgccggtt ttcagaaact gtcagcgttg
     1081 tggtgggcgt ggctatgaaa gattaccttc aacggaggca tttaatgcca tatgtaatgt
     1141 aaccgatgcc atatctcttg atacatggaa aaaacagtta aacgttttta cgatacgctg
     1201 gtggtgcagt ttgatattga agaagcatgg gcagaacaac aactgaaaaa ggtgaccaga
     1261 tagctttgtt gatttttccc gaatctgtgg taaatttgcc ctaacgatgg gcgttttatg
     1321 cctgacgtta gaagattttt tacaccccgc cgcctggcgg gttttttatg actgaaatcg
     1381 cgtcagtaca gtaaacgcgc tggtggcggt gaatacctgt ctttcagctt gctggctttt
     1441 tcgacaagag ttattggtgt gtcacgttaa ccggaaaagg gaaaaagaca tgctgaaaca
     1501 gcaggatatg accgaaaccg ccagagtggt gtttaatgaa ttaagtgcca ccgaaccggc
     1561 gacagtcggg gagattgcgc agaatactta cctttcacgt gaacgctgcc agttaatact
     1621 gacccagctt gttatggcgg ggctggcaga ctatcagttc ggttgttaca gacgccttca
     1681 gtcctgaagg cttttttatt tgtggtaaat gggcggctgg tgggtgttag gggcactcac
     1741 cagccatctg ctcatgcgtc cggatcacaa gcaaacctca ggcccactgc tttgcgcaaa
     1801 agcagaatga gcctatcaga gacaggctta atgatccatg cttagtactg taaaaatatc
     1861 cagttgtgag ttaatcaacg ccgactgcct ggaatttatc cggtcgttac ccgaaaattc
     1921 tgttgacctg atagtcacgg acccgccgta ctttaaagtg aagcctgagg gctgggataa
     1981 ccagtggaag ggcgacgatg attacctgaa gtggctggac cagtgtcttg cgcagttctg
     2041 gcgggtgctg aaacctgccg gaagtcttta cctgttctgt ggccatcgcc tggcgtctga
     2101 cattgaaatc atgatgcgtg aacgcttcaa tgtgctgaac catattatct gggcgaagcc
     2161 gtccggacgc tggaacgggt gcaacaagga aagcctgagg gcatatttcc ccgccacaga
     2221 gcgcattctg ttcgcggaac attatcaggg gccgtatcgc ccgaaagatg ccgggtatga
     2281 ggcgaagggc agggcactga aacagcatgt gatggccccg ctgatttctt actttcgtga
     2341 tgcgcgtgct gccctgggga taacggcaaa acagattgct gatgccacag gaaagaaaaa
     2401 catggtgtcg cactggttca gtgccagcca gtggcagcta ccggacgaaa gcgattatct
     2461 gaaattacag gcgctgtttg cccgggtggc agaagataag catcagcgcg gagaactgga
     2521 aaagccacat caccaactgg tcagcacata cagtgaactg aaccggcagt atacggaact
     2581 gcagagtgaa tataagcatc tgcggcggta ttttggtgtg acggtgcagg tgccgtacac
     2641 cgatgtgtgg acgcataaac cggtgcagta ctatcccggg aaacatccgt gcgaaaaacc
     2701 ggcagaaatg ctgcagcaga taatcagcgc gagcagtcgt ccgggtgacc tggttgcaga
     2761 tttcttcatg gggtcaggtt cgacagtcaa agcagcgata gcgctgggac gtcgtgcaac
     2821 tggcgttgag ctggagactg agcgttttga gcagacggtc agggaagttc agaatttagt
     2881 cagtcagaac ggatgatatt gcagaattag ttacgtaccg ttattatcct atgcccggcc
     2941 ctttagctca gtggtgagag cgagcgactc ataatcgcca ggtcgctggt tcaaatccag
     3001 caagggccac catatcacat accgccatta gctcatcggg atagagcgcc agccttcgaa
     3061 gctggctgcg cggggttcga gtcctcgatg gcggtccatt atctgcatta tgcgttgttg
     3121 gttctgatga gtgaatcatt gtcttttgag caacaggcta tcatataaga tagcctca
//
LOCUS       DAZFFE010000216         3112 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZFFE010000216 DAZFFE010000000
VERSION     DAZFFE010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.218"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(51..716)
                     /gene="yecA"
                     /locus_tag="ACHJ69_004806"
     CDS             complement(51..716)
                     /gene="yecA"
                     /locus_tag="ACHJ69_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO5017594.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(1155..1660)
                     /locus_tag="ACHJ69_004807"
                     /pseudo
     CDS             complement(1155..1660)
                     /locus_tag="ACHJ69_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461173.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
     gene            1691..1771
                     /locus_tag="ACHJ69_004808"
                     /pseudo
     CDS             1691..1771
                     /locus_tag="ACHJ69_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
     gene            1865..2107
                     /locus_tag="ACHJ69_004809"
     CDS             1865..2107
                     /locus_tag="ACHJ69_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003840043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO5017595.1"
                     /translation="MFVELVYDKRNFDGLPDAKDIILGELTKRVRRILPNADVRVKPM
                     MTLPSNNTDASKHEKEQISRAVQEMFEEADMWLVSD"
     gene            2434..>3112
                     /locus_tag="ACHJ69_004810"
     CDS             2434..>3112
                     /locus_tag="ACHJ69_004810"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO5017596.1"
                     /translation="MGIISSTNKTKSAFYEPSVDGLRALAVIIVILFHAGFKWMPGGY
                     VGVDVFFVISGYLITGILYSSNLKNTYSYKTFILSRISRLYPALISTLILTLILGFLI
                     FSPSDLERLGKTMVYTLLSASNFFFMNGSGYFDQSSETNPLLHTWSLAVEQQFYFLWP
                     LIILAGFKLGKNKLKLFIFLIGVLSLVLSQLTINSFPTANYYMPWFRAFEFAFGGIIF
                     FIEKPKRK"
BASE COUNT          828 a          741 c          631 g          912 t
ORIGIN      
        1 caaattcctt attgggtgcc agaattaacg ctgacaccca atgtgacctc ttaatgcagg
       61 cagcactgct taaatttctt accactaccg cacggacaag gatcgttacg ccccggtttc
      121 tcttctgctt tgataggttg ctgaacagct ttttcctgcg gatgcgccat ccagtatgca
      181 tgtaaatcaa gcgccgccag tcgaatagca tctacgctct cttcaaacgc ttctggcgac
      241 atcttctcta cccgctcgaa gttttcctca gtaccgtgca gcgcaatcgc ctccagcgct
      301 ggttttaacg aatcgggcaa cgttgaccag tcagaaagtg ccacgccccg catatagcca
      361 aagcaccact cctcaacaat cgtcagctcg ctgccatcga cttctcgcaa gccgaataac
      421 ggctcaaact gctccgggaa ttcgttcaga cgctctgcgg tatcggccat atgttgaaaa
      481 gccaggttca taaagcgcgt catctctttc tctgacgccc agcgcggcac atagtcagcc
      541 ccaccccaca cggcaaccag ccactgtgcc ggttcaatct cttgcggaga actcaacacc
      601 gccgtcaata aaccgtccag ctccgccaca tcaagaatgg cgtggtcagt gttgtatttg
      661 gtcagaatat cgtccagcca ttccaactca ctttcgttta acggtcccgt tttcatacgc
      721 ttttccttgt ggatctcaac tcgccagcac ctatcttaca tgccggtccg tatcagagat
      781 actttttgag tggctttgcc ggtgattaaa aattaaggag ggtgtaacga caagttgcag
      841 gcacaaaaaa accacccgaa ggtggtttca cgacactgct tattgctttg attttattct
      901 tatctttccc atggtacccg gagcgggact tgaacccgca cagcgcgaac gccgagggat
      961 tttaaatcgt gcgtgttatt atgtattatc aacaagttgc gccgtttttt cgccaataac
     1021 atatgatttt aagatcaact taatcaatag tttataagca tcaagaaggt acaatggtga
     1081 aacatttttt gctctcaaca ccgtcctaac gtgtcgtgta aatgaggtta aaatcctctc
     1141 acaatttaag ctattcacat cgccgggcag tcatcaaact ccgcgttcct ggcgtcatta
     1201 atgatgtaag tgatcacccc gaatatagcg ggtgcagaac tgtagctgtc atcatctact
     1261 ggtagcgact cccttcttcc attctccaga tttatcaagt ggggctgagg gtgagttcgg
     1321 tatcgcttta tcctgaattc cccatctatc gcgcatatca gcagcgagcc atcacaggga
     1381 gaaagtgacg catcaacaac cagtagcgcc ccctgaatta tcccttccct gaaatgcgaa
     1441 cgcgatgccc gcatgaaata agtcgctgct ggctggatga ttagctgctg atcgagggag
     1501 attcgtgttt caacataatc tgcagcaggt gaaggaaagc ccatggctat agtcctccgt
     1561 ttggattgaa cagctgaaag gtacggttct cgccttcctg cgttgataca tcgcggaatg
     1621 ttgtcacata ccactcgatc cattcgttgg cctgcttcat cgtccagttc cagttaacct
     1681 tgttcagttc ctggacaaac cgttgcgtgg ttacggtctt ccggccattt ggttcctgat
     1741 gtatcgaagc atgccaggct atttcaatat cgctgcgtct tggcatcatc acgccctctc
     1801 ttgaataccg gataaaaaca cagtataaat actgtatatc tatccagtaa aggggtaatg
     1861 cgcaatgttc gtggaactcg tttatgacaa aaggaatttt gatggtctgc cagatgcaaa
     1921 agatatcatt ctgggcgaat tgaccaagag ggttcgcagg attttaccca atgctgatgt
     1981 tcgggttaaa ccgatgatga cactgccatc gaacaacact gacgccagca agcatgagaa
     2041 ggaacagata agccgtgctg ttcaggaaat gtttgaagaa gctgatatgt ggctggtttc
     2101 agattaaacg ccttgaactg tcatattgct taagtacaga ccgtcgagac tggccatcat
     2161 tcaatacttg cactatttta gcgcccgcca gtcagcatac atacttgatg ttgcatttat
     2221 caccaatcgt atatgcgctt ttgtgcccac acgaagcctt gcaaaccgat gccaagcttt
     2281 gtgtgtcccg ttttggtctc atgccaaaaa tctacctttc cttgcttctg gtgaggtgac
     2341 ttcatgtttc tccagcacaa tcataaaatt agacacagag ccatgttatg ttaatatatt
     2401 cttctcaatg ataaattgtt ttacaggtaa gtaatgggaa ttattagctc aaccaataaa
     2461 acaaagtccg cattctatga gccatctgtc gatgggctca gagcattagc ggttataata
     2521 gttattcttt ttcatgcagg ctttaaatgg atgcctggag gatacgttgg cgtagatgta
     2581 ttttttgtta tctctggtta tctaataaca ggtattttat actcaagtaa tttaaaaaac
     2641 acatattcgt ataaaacatt tatactatca agaatatcaa gactataccc tgcgttaata
     2701 tcgactctta ttctaacatt aattcttggc tttttgatat ttagtccaag cgatttagaa
     2761 agactcggca aaacaatggt ttatacatta ttgtccgcgt caaacttttt cttcatgaat
     2821 ggttctgggt attttgacca gtcatctgaa acaaacccac tacttcatac atggtctctt
     2881 gctgttgagc aacaatttta tttcttgtgg cctttaataa ttcttgcggg ttttaaacta
     2941 ggaaaaaaca agttaaaatt atttatattt ttgatcggtg ttttatccct agtcttatcg
     3001 caactaacaa ttaactcatt ccctacagca aattattaca tgccatggtt tagggctttt
     3061 gaatttgctt ttggaggaat aattttcttc atagaaaaac caaaaaggaa aa
//
LOCUS       DAZFFE010000217         3084 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFFE010000217 DAZFFE010000000
VERSION     DAZFFE010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.219"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(200..1057)
                     /gene="sitD"
                     /locus_tag="ACHJ69_004811"
     CDS             complement(200..1057)
                     /gene="sitD"
                     /locus_tag="ACHJ69_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFO5017597.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKSWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQASGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISILVSMTGVWLSFFIDSAPAPTIVVLFAVMFIMTFTVTSINARTKENA
                     EPRDLLSSD"
     gene            complement(1054..1911)
                     /gene="sitC"
                     /locus_tag="ACHJ69_004812"
     CDS             complement(1054..1911)
                     /gene="sitC"
                     /locus_tag="ACHJ69_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFO5017598.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQLTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1908..2735)
                     /gene="sitB"
                     /locus_tag="ACHJ69_004813"
     CDS             complement(1908..2735)
                     /gene="sitB"
                     /locus_tag="ACHJ69_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFO5017599.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPGGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            complement(2735..>3084)
                     /gene="sitA"
                     /locus_tag="ACHJ69_004814"
     CDS             complement(2735..>3084)
                     /gene="sitA"
                     /locus_tag="ACHJ69_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFO5017600.1"
                     /translation="WMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQVRKVVDIV
                     KKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYIDLLKVTTS
                     TLVQGIKAGKREK"
BASE COUNT          837 a          843 c          721 g          683 t
ORIGIN      
        1 cctcgccccc caattttctc tatgttttgc cactacttcc ctcattttac tgcgctgttt
       61 gctacccctg agaatcgaca ttacttgcta tccagtccgc agcaactctc tggcaacaaa
      121 acttatcaga atcataagtc tgtgcattat tcgttcaagc gcatcgccaa taagcggatg
      181 ttgctggccg agtgttctgc taatcggatg ataacaaatc ccgtggttcg gcattttcct
      241 ttgtgcgtgc gttgatgctg gtaacggtaa acgtcatgat gaacatgacc gcgaataaga
      301 caacaattgt cggagctggc gcgctgtcga taaaaaagga cagccagaca ccagtcattg
      361 agaccaatat cgatatgcca gtcgccagca ggagcgcaat atgaaagcgt tgtgtcagga
      421 gtaccgcaat ggcaccaggc gcaatgagaa gagaaataga gagaatgata cctaccgctt
      481 tcagcgtcgc cacaatggtc agggagacca tgcacagcag cccataatgc agccatctcg
      541 tatgcaaacc gcttgcctgc gcctgttgat aatcaaagct gaacagcaaa aaatcccgcc
      601 attttacgct aataacaagt gtaaccagtc cggcaataat caccgtctgg attatatcgc
      661 cgatggttat cccgagcatg tcgccgaaaa gaatatgatc aagatgtacg tctggtttga
      721 ctgcaatata caagatcaga cctgcggcaa acatgccaga aaaaacaatc cccatcactg
      781 tatcttgctt gattcggctg ttgtctttca ggtatcccgt cgccaccgca cagaatactc
      841 cggcgacgaa tgcccctgta gccagaggta accccagaat ccaggcaagc acgatgccag
      901 gaaaaaccgc atgactcatc gcgtcaccca tcagtgccca acttttcagg accagaaaga
      961 ccgacagcag cgcacagggt atggaaacta ccagtgaaat cagcagtgcg tgactcataa
     1021 aggtgaactg cagtggctcc agtaataatg ccatcatgag cgatcctcca gcgctttatg
     1081 cgcacgccga cgatttgcca aaagcccatg ggtgggcgca aagacaaatg ctaaaagaaa
     1141 cagcagtgtc tgcgccacca cgatgattcc acctgtcgcg ccgtccagaa agtaactgac
     1201 ccacgcgccg aggaaactgg tcacactgcc aatagttaca gcaatcatga gcaggcgggg
     1261 aaaacgatcg gttaacaacc atgctgttgc accgggagtc accaccagac agatcaccag
     1321 aaaggcacca accgtttgca gcgccgcaac ggtcgaaacc gacagcaggg tgaagaagag
     1381 gatttttaat ctgcccggat gcaatccaat agcacgggcg tgattctcgt cgaaaaaggt
     1441 caccataaga tctttccatt tgaaaaaaag cacgatgatc gagagaatgc caatgatagt
     1501 gagttgcagg atatccgccg gatcgattgc cagaatattg cccaggacga tggtctgaat
     1561 gttcacggat gtcgggttca atgacaccat aaacagcccc aggccaaaaa aggacgagaa
     1621 gatcaggccg atgatggcat cttcttttaa gcgagtgagt tggttaagaa acagcatgct
     1681 gcccgctgcc agtccgccgg aaaagaaggc tcccagagaa aacggaagcc ccagcatata
     1741 tgcccccgca acacccggga caatagagtg cgacagcgca tcgccaatca gcgaccagcc
     1801 ttttagcatc agatagcatg acaggaaagc gcagagaccg ccgaccatcg ccgagaccca
     1861 catcgcattg agcatatatt catagctgaa gggttccagt agcacgttca tctttcttcc
     1921 ctctgcaccg ctgacggtcg atgtgccaca aaagggcgtt catcgtcagt aataatgctt
     1981 tcttccgagc cgttaagtgt gacatggcgc agtacgccgc taaaagccag ctccaggttc
     2041 tcggctgtaa aagtggtgtc tgtcggcccg cttgccagga cggtgccttt gaccatgacc
     2101 gtataatcgc aaaatgtcgt gacggatccg agattgtgcg ttgagacaag catcgttttg
     2161 ccttccgcgc gtaactccct caacaggctg atgattttcg cttcggtttt cacatcaacg
     2221 ccggtaaaag gttcatccag caggatcaca tcaccctgct gtgcaatcgc tctcgccaga
     2281 aagacgcgtt tcttttgtcc accggaaagc tcgccgattt gtcgatggcg aaaatccacc
     2341 atatcgacac gttccagcgc atcggtgaca atctgacgat ccctcttttt ggcgatacgc
     2401 aacataccca tatgcccata gcggcccatc atcaccacat cttccaccag tacaggaaat
     2461 gaccagtcaa cctcttctga ctgagggacg taggcaacca ggtttttctg tagcgcctgt
     2521 cgtgtgggaa tacccagaac agatatcttc ccgctggtca gacgcacaaa tcccataatc
     2581 gctttaaaca acgtcgattt tccggaaccg tttaccccaa ccagagcggc aatagatccg
     2641 cctggtactg tgaaggatgc atcgcgcagt gctgtgtgcc cgttacgcca ggtgacagta
     2701 acatcattaa cgacaatgcc tgcagattgc atcattattt ctcccttttt ccggctttaa
     2761 ttccctgtac cagcgtactg gtagtaacct tcagaaggtc gatatacgta ggtaccgggc
     2821 cgttttctgt gctcagggaa tcgacataga gcacaccacc gtagtgcgcg ccggtttcac
     2881 gcgcaacctg acgcgctggt ttatcggaaa tcgtactctc gctaaagact gccgggatat
     2941 gatttttctt aactatatca acaaccttac gtacctgctg cggtgttcct tgttgatcgg
     3001 cattaatcgg ccacagataa agctctttta gccccaaatc gcgtgcgaga taagaaaaag
     3061 ccccttcact ggtgaccatc catc
//
LOCUS       DAZFFE010000218         3052 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFFE010000218 DAZFFE010000000
VERSION     DAZFFE010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.220"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1137)
                     /locus_tag="ACHJ69_004815"
     CDS             complement(<1..1137)
                     /locus_tag="ACHJ69_004815"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFO5017601.1"
                     /translation="MDVFNTPVSRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPD
                     AITRALKKRLEHPILGYSRWQHGEYLNAIVNWYYQQYQTDIKPEWITYSPSVMYSIAK
                     AIELLTSHGDNIIVFTPVYNAFFDVIKHSERNILTASLIIKNEGGYAINWQDFDLKIK
                     SAKMVLLCNPHNPTGTVWSEEELHKIAASCTRHNVWLCSDEIHSDFVFNRSFTSALKI
                     KKEKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNIIATI
                     IAYNECEGWLKSLKAHIASNIQFTQQYLNENIPELIVRPADGCYFSWIDCSAIGYPFD
                     EFYSRLIHEGKVGIMAGHVYGTEGEGYLRLNLACGREKLYMGLTR"
     gene            complement(1137..2717)
                     /locus_tag="ACHJ69_004816"
     CDS             complement(1137..2717)
                     /locus_tag="ACHJ69_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFO5017602.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMAK
                     LIPFLAIPIIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKDKEDKAYGTFSGLIGF
                     MAMHLGTNFYLKQHDLLVVADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRKAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESNPVTLNVHEDTAIADIPTPDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEVAIKRAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGIY"
     gene            complement(2749..>3052)
                     /locus_tag="ACHJ69_004817"
     CDS             complement(2749..>3052)
                     /locus_tag="ACHJ69_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017603.1"
                     /translation="ELSQIITELIEVVYNRKVDRSSFNYSRFMMHLKYFSSRVLCNEK
                     IKQKDIGDIYEQFLEKDILLQRAIHEIERYLYATFKYELILEEKLYLSIRTKVLMD"
BASE COUNT          956 a          681 c          547 g          868 t
ORIGIN      
        1 acgcgtaagc cccatatata atttttctcg accgcaggcc aggttcagac gaaggtagcc
       61 ctccccctcc gttccataga catgccccgc cataatgccc actttgcctt catgaattaa
      121 tcgactgtag aattcatcaa aaggataacc aatagcagaa caatctatcc aggaaaaata
      181 gcaaccatca gctggcctta cgatcaattc tggaatattt tcattcagat actgctgggt
      241 gaattgaatg ttagaggcaa tatgtgcctt taatgacttt aaccatccct cacattcgtt
      301 ataggcaata atagttgcaa ttatattcag tactgatggg gagtttacaa aatcgcgata
      361 cctcgatatc gttctgaact tatgattaaa attatcgtca gttgaaatca tatacgatcc
      421 tgttagtgca ggaacattaa atgttttcga tatcgagtta aatactacta ctttttcttt
      481 ttttatttta agcgcggatg taaagctacg attaaaaaca aaatcactat ggatttcatc
      541 tgaacataac cagacattat gccttgtaca gcttgcagcg attttatgca gttcttcttc
      601 cgaccagact gttcctgtcg ggttatgagg attacataat aacaccatct ttgcgctttt
      661 aatcttaaga tcaaaatctt gccagttgat agcgtagccc ccctcatttt ttataatcaa
      721 agaggctgtc agaatatttc gctcgctatg tttaattaca tcaaaaaatg cattatagac
      781 aggtgtaaaa acgataatat tatcaccatg cgatgtcaat aactctattg cttttgcaat
      841 agagtacatc acactggggc tataggttat ccattcaggt ttaatatctg tttgatattg
      901 ttgataatac cagtttacta tcgcattcag atattcacca tgttgccaac gactgtaccc
      961 taaaatcggg tgttccaggc gtttttttaa tgctctggtg atagcgtctg gtatcggaag
     1021 atccatatct gaaatagaga atggtagtac atcttttaca ccaaatctgt cctcgcaaaa
     1081 gtcccattgg gtacaatatg ttcctttgcg ggaaacagga gtattaaaaa catccatcag
     1141 tatatcccca tatagttatc catatcccga cgcagggcga tgactttagt gccgataatg
     1201 acttgtaggg tattttgatc aagtttaaca accgcaatcc cgccagctct ttttattgca
     1261 acttcgtcaa tcaatcccat atctgcgatt gttaaacgta aacgagttac gcaattatcc
     1321 agtgacttaa tattctcttt accaccaagt gccttaacca tctgctccgc cgcttgcagc
     1381 tctgatttat caggagttgg gatatccgct atagccgtat cttcgtgaac gttcagcgtg
     1441 acaggattgg actcttcacg gcctacggtc atcaggttaa atttaagaat ggtccaacgg
     1501 aacacaaagt aataaatgaa gaaataggcc acttcgacac acaccgcgat cggccaacct
     1561 gttcttgtgc cacgcaatac gccaaagcta ataaattgaa tcagatcgcc agtaaatccc
     1621 atcttcacgc ccatatatgg cagaaccatg ttagcgaatc cattcataaa ggcatggaag
     1681 acaaacagca gtggactgac aaataagaac agaaactcaa tcggttcgct gacgccgcca
     1741 ataatcaccg taagcacccc ggaaataagc aatgctttga tagcctttct gttttgagca
     1801 tgagcagtgc gatacatcgc cagcgcggca ccagccagac catactgaat catcagtttg
     1861 ccctgctcca ttttccccgc caaatccagc ggaatgaccg cattctcttt aacgtatgcc
     1921 ataaacatgt tcagggtgcc gtaatattct tcgccgccaa tcacagccga accgccgatt
     1981 ggcgtaaagc ggaaaactga cgtcaccaga tggtttaagc caaaaggaat cgtcacacgt
     2041 tcggcaactg catagaagaa ataaccaaca ggaccggaag ttgaaatcca gtgtccaatg
     2101 agcatgaaaa gattgaaaaa cggcggccag ataaaaggaa tgatcagacc aaatccactc
     2161 atcacaatca gtgtaatgat aggcaccaga cgtgggccgc tataaaaacc taatgattct
     2221 ggaatgcgta aattaacgat ctttttatac atgctggcga ctaataaccc agcaacaatt
     2281 ccccccaaca cgctggtatt gtaggactgg atccccagaa tgatggtttg cccatgtgtc
     2341 gacatttggt cagcaacgac caataagtcg tgctgtttaa gataaaagtt cgttcccaga
     2401 tgcatcgcca taaaaccaat taagccagaa aaagtaccat aggctttatc ctctttatct
     2461 tttaataatc ctaagggaat cgctatcgca aacaatacag gtaaattaac aaaggcaaac
     2521 aaaccaagac taacaatgaa atcaagtatg gttttaatta ttggaatagc cagaaatgga
     2581 attaactttg ccatatcatc actggctaaa ccacttccca gccctagcat catgccacat
     2641 acacttagca gagcaatggg atacataaat gccttcccca ggctctgaaa aaaactccag
     2701 gctttctttt gtttcatgtg ggttatctca tataaatgtt atatataatt agtccattaa
     2761 tactttggta cgaatagaga gatataattt ttcttctaaa attaattcat atttaaaagt
     2821 ggcatacaga taccgttcaa tttcatgaat tgcgcgctgt aacaggatgt ccttttcaag
     2881 aaactgttca tagatatcac caatatcttt ctgttttatt ttttcattgc ataacacgcg
     2941 acttgaaaaa tatttaaggt gcatcataaa tctggaatag ttaaatgaac ttctgtccac
     3001 ctttctgtta taaacaactt caataagctc cgtgattatt tgcgacaact ca
//
LOCUS       DAZFFE010000219         2966 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFFE010000219 DAZFFE010000000
VERSION     DAZFFE010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.221"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            62..799
                     /locus_tag="ACHJ69_004818"
     CDS             62..799
                     /locus_tag="ACHJ69_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000967278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5017604.1"
                     /translation="MLYSNILAHARRCAPAESCGFVVRTPEGERYIPCVNISAEPEAY
                     FRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHKFR
                     CVPHLAGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHRGDDWWRNGQNLYLDNMEGTGF
                     YRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTH
                     SVWRHRHWHASAFTGIYNDLAAASACM"
     gene            796..1377
                     /locus_tag="ACHJ69_004819"
     CDS             796..1377
                     /locus_tag="ACHJ69_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5017605.1"
                     /translation="MNTAAEAIRALSMQMPGFRRQMNEGWYQIRIAGDDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALSAGGFS
                     ATTMLFSLGASMILGGAAQMLAPKAKTPDYRVTDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGKVVVIGRQG"
     gene            complement(1618..2145)
                     /gene="sodC"
                     /locus_tag="ACHJ69_004820"
     CDS             complement(1618..2145)
                     /gene="sodC"
                     /locus_tag="ACHJ69_004820"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO5017606.1"
                     /translation="MNRKSLAAIAMVLATSCGYAATIKVPMNLVNADGKEVSAGTITI
                     QETSYGLLFTPDLHSLPQGIHGFHVHEKGSCAPALKDGVSVAALAAGGHFDPKQTGKH
                     LGPWSPDGHLGDLPALYVTHDGKANYPVLAPKLKELTEISGRSLMIHIGGDNYHDHPD
                     PLGGGGARMGCGIIP"
     gene            2281..>2966
                     /locus_tag="ACHJ69_004821"
     CDS             2281..>2966
                     /locus_tag="ACHJ69_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO5017607.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETVLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVQTTSKGDRNPTSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLALVILDNLPPRPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGG"
BASE COUNT          684 a          730 c          889 g          663 t
ORIGIN      
        1 ctactaacag attaccaacg aaatgaccca gcctcggctg ggttttttat caggagttct
       61 catgctctat agcaatatat tggcacatgc ccggcggtgt gcgcctgcgg agtcgtgcgg
      121 cttcgtggtg agaacgccgg agggggagcg gtatatccct tgtgtgaata tttccgcaga
      181 gccggaggcg tattttcgta ttgcaccgga agactggctg cgggcagaga tgcaggggga
      241 gattgtggca ctggtccaca gtcatcccgg tggtctgccc tggctgagcg aggctgaccg
      301 gcggctgcag ataaaaagcg cactgccctg gtggctggtc tgccggggtg acattcacaa
      361 attccgctgt gtgccacacc tggcgggacg gcgctttgag cacggggtga cggactgtta
      421 cacgctgttc cgggatgctt atcatctggc ggggactgaa atgccggatt ttcatcgcgg
      481 ggatgactgg tggcgcaacg gccagaacct gtacctggac aatatggagg gaacgggctt
      541 ttaccgggtg cccctgtcct ctgcacaggc gggcgatatt ctgctgtgct gctttggtgc
      601 ttcggtaccg aaccatgccg ccatttactg cggcaacggt gagctgcttc accatctgcc
      661 tgaacaactg agtaaacggg agaggtattc cgaaaaatgg caacgacgaa cgcattctgt
      721 ctggcgtcac cgccactggc acgcatctgc cttcacgggg atttacaacg atttggccgc
      781 cgcctcagcc tgtatgtgaa cacggcagcg gaagccatcc gcgccctgtc gatgcagatg
      841 ccgggctttc gccgtcagat gaacgaaggc tggtaccaga tacgtattgc cggtgatgac
      901 acggcaccgg aggcggtgta cgcccgtctt cacgaacagc tgggtgaggg aacggtcatc
      961 cacattgtgc cgcgactggc cggggccgga aagggtggac tgcagattgt gctgggggcg
     1021 gcagccatcg tgggctcttt cttcacggcc ggtgcctcga tggcgttatg gggttcagcc
     1081 ctgagtgccg gtggtttttc tgccaccacg atgctgtttt cactgggggc cagcatgata
     1141 ctgggcggtg cggcacagat gctggccccg aaggcaaaaa caccggatta ccgcgtaacg
     1201 gataacggca gacagaacac gtacttttcc tcgctggata acatgattgc ccaggggaac
     1261 ccgatgccgg tgccttacgg ggaaatgctg gttggctccc ggcgaatctc ccaggacatc
     1321 agtacccgtg atgaaggcgg gggcggaaag gtcgtggtta tcgggcggca ggggtaaaaa
     1381 gaataaaaaa atcccgcagt gatcgcggac aggaactgcg ggagagttac gaagattaag
     1441 tgtaaggaat tattcttatg tcacgacaaa aaaattaacg cagagaaatt ataagtgcca
     1501 cagggatttt gtgaaaatgt gaagatattc agagtttttc tgtttgttct tctggcgaga
     1561 gcggattcat tgactggtgg agaaaaggta agggggcagc tccccttacc agacttgtta
     1621 tgggatgata ccacagccca ttcttgcacc gccgccgccg agtggatctg gatggtcatg
     1681 atagttgtca ccgccaatgt ggatcataag agaccgtccg cttatttctg ttaattcttt
     1741 cagttttggt gcgagaaccg gatagtttgc ttttccgtca tgagtcacgt acagagccgg
     1801 gagatcgccc aggtgaccat caggagacca gggaccaaga tgttttccag tctgtttcgg
     1861 gtcaaagtga ccgccagctg ctaatgccgc aacagatact ccatctttca gagccggcgc
     1921 gcaacttccc ttttcgtgca catgaaatcc atgaatgccc tgaggtaaag agtgcagatc
     1981 tggtgtgaac aggagaccat agctggtctc ctggatagtt attgttccgg cgctcacctc
     2041 tttaccatcg gcattaacaa ggttcatggg gacttttatt gtggcggcat atccacatga
     2101 tgttgccagc accattgcaa tagcagcaag acttttacgg ttcataaaac cctcattaat
     2161 tattttaaca gactgagttt actgactgca ggagtacaaa cagcgttctg atgatatcgc
     2221 gcaatagtta ttgaatgtga ctattgcttc gattgatcat atcaattaac aggagttatc
     2281 atgggtaaag gtggcggcaa ggcgcacaca cctcgtgagg cgaaggataa tctcaaatcc
     2341 acgcagatga tgagcgtgat tgatgcgatt ggtgagggac cgatagaagg tccggtgaag
     2401 ggactgcaga gtatcctggt gaacaaaacc ccgctgacgg acacggacgg taatcccgtg
     2461 atacacggtg tgacggcggt ctggcgcgcc ggggagcagg agcagacacc accggaaggc
     2521 tttgagtcct ccggagctga aactgtactg ggcgtggaag tgacgaaggc aaaaccggtg
     2581 acgcgcacca ttacgtccgc gaacattgac cgcctgcggg ttaccttcgg ggtgcagtca
     2641 ctggtgcaga ccacgtcaaa gggtgaccga aacccgacat ccgtccgcct gctgattcag
     2701 ttacagcgta acggtaactg ggtgacagaa aaggatgtca ccattaacgg caagaccacc
     2761 tcgcagtttc tggcgttggt gattctggat aatctgcctc cccgtccttt taacatccgg
     2821 atggtccggg agacggcgga cagcaccacg gaccagttgc agaacagaac actctggtca
     2881 tcgtacaccg aaatcatcga tgtgaaacag tgctacccga acacggcgat tgtggggctg
     2941 caggtggatg cggagcagtt cggtgg
//
LOCUS       DAZFFE010000220         2904 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZFFE010000220 DAZFFE010000000
VERSION     DAZFFE010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.222"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            93..2471
                     /gene="ppsA"
                     /locus_tag="ACHJ69_004822"
     CDS             93..2471
                     /gene="ppsA"
                     /locus_tag="ACHJ69_004822"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO5017608.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGGNV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2528..>2904)
                     /locus_tag="ACHJ69_004823"
     CDS             complement(2528..>2904)
                     /locus_tag="ACHJ69_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HFO5017609.1"
                     /translation="GYIKITRRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMP
                     DERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTVSGKI
                     QKFLLRKDIMRRLTQDVCEEIE"
BASE COUNT          680 a          723 c          795 g          706 t
ORIGIN      
        1 ttcaccgttt ttttcatctg gttaaatatg caaagataaa tgcgcagaaa tgtgtttctc
       61 aaaccgttca tttatcacaa aaggattgtt cgatgtccaa caatggctcg tcaccgctgg
      121 tgctttggta taaccaactc ggcatgaatg atgtagacag ggttgggggc aaaaatgcct
      181 ccctgggtga aatgattact aacctttccg gaatgggtgt ttccgttccg aatggtttcg
      241 ccacaaccgc cgacgcgttt aaccagtttc tggaccaaag cggcgtaaac cagcgcattt
      301 atgaactgct ggataaaacg gatattgacg atgttaccca gcttgcgaaa gcgggcgcgc
      361 aaatccgcca gtggattatc gacactccct tccagcctga gctggaaaac gccatccgcg
      421 aagcctatgc acagctttcc gccgatgacg aaaacgcttc gtttgcggtg cgttcctccg
      481 ctactgcaga agatatgccg gacgcttctt ttgccggtca gcaggaaact ttcctcaacg
      541 ttcagggttt tgacgccgtt ctcgtggcag tgaagcatgt atttgcttct ctgtttaacg
      601 atcgcgccat ctcttatcgt gtgcaccagg gttacgacca tcgtggcgtg gcgctctccg
      661 ccggtgttca gcggatggtg cgctccgatc tcgcatcttc tggcgtgatg ttctccattg
      721 ataccgaatc cggctttgac caggtggtgt ttatcacttc cgcatggggc cttggtgaaa
      781 tggtcgtgca gggtgcggtt aacccggatg agttttatgt gcataaaccg acacttgcgg
      841 cgaatcgccc ggctatcgtg cgccgcacca tggggtcgaa aaaaatccgc atggtttacg
      901 cgccgaccca ggagcacggc aagcaggtta aaatcgaaga cgtaccgcag gaacagcgtg
      961 acatcttctc gctgaccaac gaagaagtgc aggaactggc aaaacaggcc gtacaaattg
     1021 agaaacacta cggtcgcccg atggatattg agtgggcgaa agatggccac accggcaaac
     1081 tgttcattgt gcaggcgcgt ccggaaaccg tgcgctcacg cggtcaggtc atggagcgtt
     1141 atacgctgca ttcacagggt aagattatcg ccgaaggccg tgctatcggt catcgcatcg
     1201 gcgcgggtcc ggtgaaagtc atccatgaca tcagcgaaat gaaccgcatc gaacctggcg
     1261 acgtgctggt tactgacatg accgacccgg actgggaacc gatcatgaag aaagcatctg
     1321 ccatcgtcac caaccgtggc ggtcgtacct gtcacgcggc gatcatcgct cgtgaactcg
     1381 gcattccggc ggtagtgggc tgtggtgatg caacagaacg gatgaaagac ggcgggaacg
     1441 tcactgtttc ttgtgccgaa ggtgataccg gttacgtcta tgcggagttg ctggaattta
     1501 gcgtgaaaag ctccagcgta gaaacgatgc cggacctgcc gttgaaggtg atgatgaacg
     1561 tcggtaaccc ggaccgtgct ttcgacttcg cctgcctgcc gaacgaaggc gtgggccttg
     1621 cgcgtctgga atttatcatc aaccgtatga ttggcgtcca cccacgcgca ctgcttgagt
     1681 ttgacgatca ggaaccgcag ttgcaaaacg aaatccgcga gatgatgaaa ggttttgatt
     1741 ctccgcgtga attttacgtt ggtcgtctga ctgaagggat cgcgacgctg ggtgccgcgt
     1801 tttatccgaa gcgcgtcatt gtccgtctct ctgattttaa atcgaacgaa tatgccaatc
     1861 tggtcggtgg tgagcgttac gagccagatg aagagaaccc gatgctcggc ttccgtggcg
     1921 cgggccgcta tgtttccgac agcttccgcg actgcttcgc gctggagtgt gaagcagtga
     1981 aacgtgtgcg caacgacatg gggctgacta acgttgagat catgatcccg ttcgtgcgta
     2041 ccgttgatca ggcgaaagcg gtggttgagg aactggcgcg tcaggggctg aaacgtggtg
     2101 agaacgggct gaaaatcatc atgatgtgtg aaattccgtc caacgccttg ctggcagagc
     2161 agttcctgga atatttcgac ggcttctcaa ttggctcaaa cgacatgacg cagctggcgc
     2221 tcggtctgga tcgtgactcc ggcgtggtgt ctgaactgtt cgatgagcgc aacgatgcgg
     2281 tgaaagcact gctgtcgatg gcgattcgtg ccgcgaagaa acagggcaaa tatgtcggga
     2341 tttgcggtca gggtccatcc gaccacgaag actttgctgc atggttgatg gaagagggga
     2401 tcgatagcct gtctctgaac ccggacaccg tggtgcaaac ctggttaagc ctggctgaac
     2461 tgaagaaata aaataaatac ccggcggcgt tttgtcgccg ggtttatgtg atccccgaag
     2521 atgaaactta ttcaatctct tcacagacat cctgagttaa tcgccgcata atatctttcc
     2581 ttaacaaaaa cttttgtatt ttacctgaga cagtgcgcgg tagtttttcg attaccacga
     2641 tatgttcagg atatttatat tttgcgaccc gtttacggct aaaaaaagcc accacctctt
     2701 ccagcgataa tgaatgatgc ggcgctttca gcacgacata agcgcatgaa cgttcaccta
     2761 aacgttcatc gggcatcgca accacacagg catcgtgaat tttaggatgc tgcaataaaa
     2821 tatcttctac ttcacggctg ctaatatttt cgccgccgcg gacaataata tctttcttgc
     2881 gcctggttat ttttatatag ccag
//
LOCUS       DAZFFE010000221         2873 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZFFE010000221 DAZFFE010000000
VERSION     DAZFFE010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.223"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..2691)
                     /gene="chiA"
                     /locus_tag="ACHJ69_004824"
     CDS             complement(<1..2691)
                     /gene="chiA"
                     /locus_tag="ACHJ69_004824"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HFO5017610.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDGK
                     IYENAWWVSSTNCPGKAKANDATSPWRLKRTATAAEISQFGNTLSCEKSGSSSSSTSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAIYKNAWWVASSNC
                     PGDAKGNDASNPWRYVRAATATEISETSNPQSCTSAPQPAPDVKPAPDVKPAPDVQPA
                     PADKSNDNYAVVAWKGQEGSSTWYVIYNGSIYKNAWWVGAANCPGDAKENDASNPWRY
                     VRAATATEITQYGNPGSCSVKPDNNGGAVTPVDPTPETPETPVTPTPDNNEPSTPADS
                     SNDYSLQAWSGQEGSEIYHVIFNGNVYQNAWWVGSEDCPRGTSVENSNNPWRLVRTAT
                     AAEMSQYGNPTTCEIDNGGVIIADGFQASKAYSANSIVDYNDAHYKTSVDQDAWGFVP
                     GGDNPWKKYEPAKAWSASTVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNS
                     RPWKPLGKTQSYSNEELNNAPQFNPETLYASDTLIRFNGENYISQSKVQKVSPSDSNP
                     WRVFVDWTGTKERVGTPKKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVV
                     SKDANTCLPTWGTAYGMQNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDL
                     MQHYYDIVDNLNLRVLDFDIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTL
                     PILPTGLTPEGMNVLSDAKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSA
                     IANLHAQLKGLYGNKSDAEIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLG
                     MVGIWSIARDLPGGTNLSPEFHGLTKEQAPKYAFSEIFAPF"
BASE COUNT          707 a          738 c          660 g          768 t
ORIGIN      
        1 aaacggcgcg aagatttcgc taaatgcgta cttcggagcc tgttctttag tcagaccgtg
       61 gaattccgga gacaggttag tgccgcccgg caggtcgcgc gcgattgacc agataccaac
      121 cataccgaga ttacgcttct gcgcatcctg catgaccaga cgagcatcag agagatagaa
      181 cacctcaccc tgaacgtcgt tcacgccaac catcggcgtg gtacccatca tggcgtcaat
      241 ttctgcatca ctcttattgc catagaggcc tttcagctgc gcatgcaggt tggcaatcgc
      301 agacgttgca cacttaccgt ggatgttctg gccttcggtg tttgcagact gacaaatagc
      361 gttaccgtag tccattgtca tcacgttcac acccgccagc tcaacacctt tcgctttggc
      421 atcgctcagg acattcatcc cttccggcgt caggcctgtc ggcagaattg gcaaggtata
      481 ccagatagca atatctttgc cttctgactt ccatttatcc tgcactttct tcacagcaag
      541 gttacgacgt tcaatagatg cctgatccgc aacccaggtg ccttcgatat cgaagtccag
      601 gactctgagg ttcaggttat caacgatgtc ataataatgc tgcattagat cgtcaacgtt
      661 cttacaggaa gctgccagcg gggcattatt agcaccaccg atagacagca tcacatcacc
      721 gccagcctca cgcagagctt tgattttgct gtactgagcg taattctgca taccgtaagc
      781 ggtaccccat gtcggtagac aggtgttcgc atctttactc accacaaacg ccagcgtgaa
      841 gtggttgacg ttatgattct tagccagcgc agccaaatcc gggatcgtat tcagcgtaaa
      901 gtcgacatac ggtgcataaa cgtgtttcgg ccacgctttc ttcggcgtac ctacgcgctc
      961 tttggttccg gtccagtcaa caaaaacacg ccacgggttg ctgtcagaag gagaaacttt
     1021 ctgcacttta ctctgagaaa tgtagttctc accgttaaag cgaatcagcg tatcactggc
     1081 ataaagcgtt tctggattaa actgcggcgc attattcagc tcttcgttgc tatagctctg
     1141 agtcttacct aacggcttcc acgggcgact attgctaccg gtggcgtttt ggttcgcaac
     1201 cagagcaggg ttgtcacttt gcgtccagaa cagcgcttca taagcctgcc catcaacaac
     1261 aacgcgatca cctttcacgt acacggtgga agcggaccat gctttcgccg gttcgtattt
     1321 cttccacggg ttatcgccgc cggggacaaa tccccatgcg tcttgatcga cagaggtttt
     1381 ataatgtgca tcgttataat ctacgatgct gttcgcgctg tacgctttgc tggcctggaa
     1441 accatccgca ataatgacgc cgccattatc aatttcacag gtagtcgggt taccgtactg
     1501 actcatttcc gcagcggtag ctgtacgcac gaggcgccat gggttattgg agttttctac
     1561 actggtgcca cgcgggcaat cttcagaacc aacccaccag gcattctgat aaacattacc
     1621 attgaagata acatggtaaa tttcgctgcc ttcctggcca ctccacgcct gcagtgagta
     1681 atcgttactg ctatccgctg gtgttgatgg ctcgttgtta tccggagttg gagtaactgg
     1741 agtttccggt gtttccggag ttggatcaac cggagtcaca gcaccgccat tattatccgg
     1801 cttaacggaa caggagcctg ggttaccata ctgagtgatt tctgttgccg ttgccgcgcg
     1861 aacataacgc catgggttgc tggcatcgtt ttctttcgca tcgcctgggc aatttgccgc
     1921 gcctacccac caggcgttct tataaatgct gccgttgtag ataacgtacc atgtagaaga
     1981 accttcctga cctttccagg ctacgacagc atagttgtcg tttgacttat cagctggggc
     2041 tggctgaaca tcaggagccg gtttaacgtc cggtgccggt ttcacatccg gtgcaggctg
     2101 tggtgctgaa gtacaagact gtggattact ggtttctgag atttccgttg ccgtagcggc
     2161 acgaacataa cgccatgggt tgctggcatc attgcctttc gcatcacccg ggcagttaga
     2221 agaggctacc caccaggcat ttttatatat cgcaccatta aagacgacat accaggtttg
     2281 accgccctgc tgtttattcc aggcaactac agatgaatta gacggaacag tgccctgtgc
     2341 tggtgtagcc gaaccgccat tagcaggcgt attagatgct ggcgtatttg aagttgaaga
     2401 agatgagctt ccgctctttt cgcaggaaag tgtattgcca aactgactaa tttcagcagc
     2461 tgttgcggta cgctttaaac gccacgggct agttgcatca tttgctttcg cttttcccgg
     2521 gcaatttgta gaagaaaccc accaggcatt ttcataaatt ttgccatcga aaataacctg
     2581 atatttatta ccaccttgtt ggttattcca tgcttccatt gccaatgctg gcagagcgga
     2641 acacaccagc cccataccaa tcatagattt agtaaatata tttaatttca ttataatccc
     2701 ttgtaacgta aaaactgcaa aacaaaagtc ctacgcagga attatttacg ttttacgaga
     2761 acccttcaat atgaattaaa gtgcggcatg aaaaaataag aaaaaagcct gacaaatgaa
     2821 gcattttaaa aacagaaaca ttcatattta aaatattaaa ttgaattgat att
//
LOCUS       DAZFFE010000222         2862 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZFFE010000222 DAZFFE010000000
VERSION     DAZFFE010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.224"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..482)
                     /locus_tag="ACHJ69_004825"
     CDS             complement(<1..482)
                     /locus_tag="ACHJ69_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO5017611.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNSMGNDQIRTVNLIKSARVGYTKMLLGVAGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTITLKRFSSGVGFWCLG
                     G"
     gene            complement(454..963)
                     /locus_tag="ACHJ69_004826"
     CDS             complement(454..963)
                     /locus_tag="ACHJ69_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5017612.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            complement(1769..2290)
                     /locus_tag="ACHJ69_004827"
     CDS             complement(1769..2290)
                     /locus_tag="ACHJ69_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO5017613.1"
                     /translation="MNIVPLNYKGEIVSFNTDGWINVTGVAERFGKRIDNWMRLAETL
                     EYVRALDEALTGKESQILHPSQSRYVKTSKARKDRGGGTWLHPKLSVAFARWCDARFA
                     VWCDLHIDSLLRGELTEQQKYEQACRIRDDRKSKASNGAREMARWRWDKPVIEANVEY
                     WREQLQLTLDIAC"
     gene            complement(2492..>2862)
                     /locus_tag="ACHJ69_004828"
     CDS             complement(2492..>2862)
                     /locus_tag="ACHJ69_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO5017614.1"
                     /translation="ITYKVQRDKKARELELANATITDMQVRQRDVAALDAKYSRELAD
                     ARAENETLRADVAAGRKRLQVAATCAKSTTGASSMGDGESPRLTADAELNYYRLRSGI
                     DKITAQVNYLQEYIRTQCLK"
BASE COUNT          691 a          745 c          632 g          794 t
ORIGIN      
        1 ccgcccaggc accagaagcc cacacccgat gaaaagcgtt tcagcgtgat ggtattatca
       61 cgatgtttac gtcccagcca gggagaaagt tttttcaggc agggaacatc gcgaatcgtc
      121 gcctccacgt gagacttcat aaaatcttca gcggcagaat ccgtgggctg aaaaagcaga
      181 ctgtttcggg atttatgctc aataaaatac ccggcgactc ccagcaacat ctttgtatag
      241 ccaacacggg cagatttaat cagattaaca gtccggatct gatcattccc catgctgttc
      301 atgatggcga tctggaacgg cagcgttttc cattctccct caccatatga agattcttta
      361 ggcagataat aattttgatc agcccattca actggcgtca ccggcaatgc ccttatcagg
      421 ggctgtaatg ctgttgtgac agcactcatc atattattca gttgttgctc tgatatattc
      481 atcgagtaaa tccggtaatt tatcccccgc ccgcgcacac tgatttgccc ccttcgcaat
      541 aagggttttc agatggtcaa gatggcgcgg tgttaaatca ggaaactgtc gctgcatgga
      601 taaagggatg gaatcaagcg tactggataa cgccattgcc agcttgctga gggcaaaaat
      661 acagaacccg gtgtcaataa gttttccttt tgacacctca ttttttaact gctgtgtaac
      721 agcctgttct gctgtcagtt cccatctggc aataagcaat ttctcctcat agtcgtcttc
      781 gctatcgcca tcaggcacat cgtttttact tcttctcaga tacgatatgt aaaaatcgcg
      841 ccaggcatcc agatccagtt gccctcgctt attcgatatc ggggcacccg gcaatttctg
      901 caatctgcga agctggcgat cggtcagact taaatgcctg gcaacttcag tctgcgtagc
      961 cactcctcac ctcgcaaaaa ctctcacctc acaatcacaa caaaaccggt catgtccggt
     1021 tttagtgtct attttttgcg catgtccggt tcatggaaag catgttttta tatttttcat
     1081 atggttaact tgcagagaaa ccggacatgg atcccggaaa attttcataa atagtgaaaa
     1141 tccgcgaggt cgccgccccg taacggcccg gatcgccgga aaggacccgc aaaaatgata
     1201 atggttatca ttttcaatgt agtccgattt cttccaccat cgcaccggac aggcgactat
     1261 gaggggacaa cgccgcgctc cgttaacgcg gtaaaccccg gtgtgtatcg tttttgatta
     1321 tttccgcaca ctcgcgcaga ggagttcccc gtcgggctgc ggtctctgtt aatgcaggaa
     1381 tacggcgacg atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga
     1441 tatcagttaa gttatcccgt gtagggtata agccattgtc gagaccactc attgaatggc
     1501 ctctgcaata accgatgtct ttccatcagt ccgccaccac aaagaatctt ttttgcctta
     1561 aggcaggagc ttcatctttc agtggctgcc agtgttattt ccccacttac tggcttgggt
     1621 tgtttcgtgg tactgccgta actggttgcc aagaataaat tccggtttca ttatcaagcc
     1681 cacccgtaga tgggctttgt aatggatagc cgttgctcag ttctcgtaat gctttgattt
     1741 ttccgataac gcagttttgc gtttgccatc agcacgcgat atcgagagtc aactgcagtt
     1801 gctcgcgcca gtactcaaca tttgcttcaa taaccggctt atcccatcgc cagcgagcca
     1861 tctctcttgc cccattgctg gcttttgatt tccggtcatc gcgaatgcga catgcttgct
     1921 catatttctg ctgctcagtc agttcaccgc gaagcagact atcaatgtgc aggtcgcacc
     1981 acacagcaaa acgagcatca caccaacggg caaatgcaac tgaaagtttt ggatgtagcc
     2041 acgtaccacc acccctgtcc tttcgtgcct tgctggtttt tacatacctc gattgtgagg
     2101 gatgtaaaat ttgagattct ttcccggtca acgcttcgtc taaagcacga acgtattcaa
     2161 gcgtttctgc caaacgcatc cagttatcaa tgcgttttcc aaatctctca gcaacacctg
     2221 tgacgttgat ccaaccatca gtgttgaaac tgacaatttc acctttgtaa ttaagtggca
     2281 cgatattcat aacgtttacc taccatttga aatgaacctt tgccgcatag gaaaccagcc
     2341 caccgaggct cgccagcact aactggtatc ctcaaaggcc cattccaaag gggcaggttc
     2401 ggtgtaaaaa acatgcgttg cggtacgcat ttattgcaaa aagccccgca tcgcgaggct
     2461 cattaaattg actttgtgat ttgcaaaaaa attatttcag gcattgcgtc ctgatgtact
     2521 cctgcaggta gttaacctgc gcggttatct tgtcgattcc acttcggaga cggtaataat
     2581 tgagttcagc atctgctgta agtcttgggc tttctccatc gcccatgctg ctggctccgg
     2641 tcgttgactt tgcacaggtg gcggcgactt gcaggcgctt acgaccagca gcaacatcag
     2701 cacgcagagt ttcattttca gctctcgcat cggctaattc cctcgagtat tttgcatcga
     2761 gcgcagcaac atcgcgctgg cgcacctgca tatcagtaat ggtggcgttt gccagctcca
     2821 gctctctggc ttttttatcg cgctgcactt tgtaggtgat gg
//
LOCUS       DAZFFE010000223         2829 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFFE010000223 DAZFFE010000000
VERSION     DAZFFE010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.225"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..314
                     /locus_tag="ACHJ69_004829"
     CDS             <1..314
                     /locus_tag="ACHJ69_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017615.1"
                     /translation="DFTQINSDSDAERNDHAVSFIRDAMNSEEHIIIFNCVGWPDLGG
                     GSAVSQFAMMARKFGKQLIVAVSEKDAIKLKDNFNIIGTLSYGKENFIAMSHTKSEFC
                     S"
     gene            513..662
                     /locus_tag="ACHJ69_004830"
     CDS             513..662
                     /locus_tag="ACHJ69_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5017616.1"
                     /translation="MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            947..1204
                     /locus_tag="ACHJ69_004831"
     CDS             947..1204
                     /locus_tag="ACHJ69_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO5017617.1"
                     /translation="MSQTVNAETSSSGKKRPYRRGNPVPARERQKASLARRSATHKAF
                     HAVIQLRLKEKLSELADEDGITQAQMLEWLIESEVKRRKSL"
     gene            1438..1512
                     /gene="tap"
                     /locus_tag="ACHJ69_004832"
     CDS             1438..1512
                     /gene="tap"
                     /locus_tag="ACHJ69_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO5017618.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1505..2362
                     /gene="repA"
                     /locus_tag="ACHJ69_004833"
     CDS             1505..2362
                     /gene="repA"
                     /locus_tag="ACHJ69_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO5017619.1"
                     /translation="MTDLQQTYYRQVKNPNPVFIPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDI
                     SEEAVASARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFCSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREISEGRFSASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT          760 a          671 c          726 g          672 t
ORIGIN      
        1 atgatttcac tcagattaac agtgattcag atgcagagag aaatgatcat gccgtctcct
       61 ttatcaggga tgcaatgaat agtgaagagc atataattat ttttaattgt gttgggtggc
      121 ctgatttagg tggtggatca gcagtatcgc agtttgcaat gatggccaga aaatttggta
      181 aacaacttat tgtagctgta agtgaaaagg atgccataaa attaaaagac aactttaata
      241 ttattggaac gttgtcatat ggtaaagaaa attttattgc catgagccat acgaaaagcg
      301 agttctgtag ttaaaagcgg gaaatggagg taatggtcag caacagggaa tgtggtatta
      361 tcgcggcggg tgtctgagcc tttctggttc aggcaagacg caggtaccag aaatgcgaag
      421 accccacttg ttaatccatt aactcgtgag gcctgcatga agtacgtagc actactgatt
      481 gtagcctctt ccttgcagag aggtcaaagt ttatgacgaa atataccctt atcgggttgc
      541 tcgccgtgtg cgccacggtg ttgtgttttt cactgatatt cagggaacgg ttatgtgagc
      601 tgaatattca caggggaaat acagtggtgc aggtaactct ggcctacgaa gcacggaagt
      661 aagctgccgg gcgggggcgg aagtccccgc tttccggaag tgtggggtat ttcaggggca
      721 gacacccgac atgccagaaa ccgccggtcc cgcccggggc cggcacacag gttaaggcat
      781 ttcctgtttt ttcagccatt tcgcaacaaa aatcacatta aacaacagta atcagacatg
      841 atttgtgcgc caacacagat cttcgtcaca attctcaagt cgctgatttc aaaaaactgt
      901 agtatcctct gcgaaacgat ccctgtctga gaagagagga ggcgagatgt cgcagacagt
      961 aaatgcagag acttcctcat cgggaaaaaa acgaccttac agaaggggta atcctgttcc
     1021 tgcgagagaa cgacaaaaag cgtctcttgc aagaaggagt gccactcata aagcgtttca
     1081 tgccgtcatt cagcttcgac tgaaagaaaa gttaagtgag cttgctgatg aagacgggat
     1141 cactcaggca cagatgcttg agtggctgat agagtcagag gttaagcgca gaaaatcttt
     1201 gtgaatattt gcgtttcttg ctgtttcagt gatgagatgt tagattgctg atcgttttaa
     1261 ggaattttgt ggctggccac gccgtaaggt ggcagggaac tggttctgat gtggatttac
     1321 aggagccaga aaagcaaaaa ccccgataat cttcagaaag tttggcgact gagaagatta
     1381 ccggggccca cttaaaccgt atagccaaca attcagctat gcggggagta tagttatatg
     1441 cccggaaaag ttcaagactt ctttctgtgc tcgctccttc tgcgcattgt aagtgcagga
     1501 tggtgtgact gatcttcaac aaacgtatta ccgccaggta aagaacccga atccggtgtt
     1561 tataccccgt aaaggtgccg gaacgctgaa gttctgcgaa aaactgatgg aaaaggcggt
     1621 gggcttcact tcccgttttg atttcgccat tcatgtggcg catgcccgtt cgcgtggtct
     1681 gcgtcgacgc atgccaccgg tgctgcgtcg acgggctatt gatgcgctcc tgcaggggct
     1741 gtgtttccac tatgacccgc tggccaaccg cgtccagtgt tccatcacca cactggccat
     1801 tgagtgcgga ctggcgacag agtccggtgc aggaaaactc tccatcaccc gagccacccg
     1861 ggccctgacg ttcctgtcag agctgggact gattacctac cagacggaat atgacccgct
     1921 tatcgggtgc tacattccga ccgatatcac gttcacaccg gcgctgtttg ccgctctcga
     1981 tatctcagaa gaggcggtgg cttccgcacg ccgcagccgt gtggaatggg aaaacagaca
     2041 gcgcaaaaag caggggctgg ataccctggg tatggatgag ctgatagcga aagcctggcg
     2101 ttttgtgcgt gagcgtttct gcagttacca gacagagctt aagtcccgtg gaataaaacg
     2161 tgcccgtgcg cgtcgtgatg cgaacaggga acgtcaggat atcgtcaccc tggtgaaacg
     2221 gcagctgacg cgtgaaatct cggaagggcg cttctctgcc agtcgtgagg cggtaaaacg
     2281 tgaagtggag cgtcgtgtga aggagcgcat gattctgtca cgtaaccgca attacagccg
     2341 gctggccacc gcttccccct gaaagtgacc tcctcagaat aatccggcct gcgccggagg
     2401 aatccgcacg tctgaagccc gccagtacag aaaaaaacag caccacatac aaaaaacaac
     2461 ctcatcatcc actttcagat gcatccggtt ctctctgttt ttgatacaaa acacgcctca
     2521 cagacgggga attttgctta tccacattaa actgcaaggg acttccccat aaggttacaa
     2581 ccgttcatgt cataaagcgc cagccgccag cgttacaggg tgcaatgtat cttttaaaca
     2641 cctgtttata tctcctttaa actacttaat tacattcatt taaaaagaaa acctgttcat
     2701 tgcctgtcct gtggacagac agatatgcac ctcccaccgc aagcggcggg cccctaccgg
     2761 agccacttta gttacaacac tcagacacaa ccaccagaaa aaccccggtc cagcgcagaa
     2821 ctgaaacca
//
LOCUS       DAZFFE010000224         2769 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFFE010000224 DAZFFE010000000
VERSION     DAZFFE010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.226"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..601)
                     /locus_tag="ACHJ69_004834"
     CDS             complement(<1..601)
                     /locus_tag="ACHJ69_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO5017620.1"
                     /translation="MTTNKTSLSRLTKVRHRNELNSTLSTLPMAAKKVLFLAMCQINS
                     KNEFDDDHIFYVTVADYIKWVQVKPDAAYLALRDGSNILDTTLLKLKHDEILELSSDL
                     GFKFTKSNVPDSMNLSLTVFSTYYRNEGRVGIKFTKEAKRFLCKLIGEEKRYTTQVLL
                     SVVRLTSVNAASLYQLIRKIYSNNSRANSFEMTIDELKDE"
     gene            1856..2191
                     /locus_tag="ACHJ69_004835"
     CDS             1856..2191
                     /locus_tag="ACHJ69_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004026069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5017621.1"
                     /translation="MPYTAKRYQTENGEVPYTDWMKKLRRKDQTAALKVDSRIARAMG
                     GNFGDHKFERDGVWELRVDYGPGYRVYYSIEDGEIILLLIGGNKKTQTADLDKAVSYL
                     QDFKKRSKK"
     gene            2188..2538
                     /locus_tag="ACHJ69_004836"
     CDS             2188..2538
                     /locus_tag="ACHJ69_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5017622.1"
                     /translation="MTATKNVKSQKNDYPASVDHNAAMIEELRADPDYANAYLANALE
                     EINEPGGLGGFLVALRQVIEARGGISEAARKSGLARQSIYRALSPNGNPTITTLAQLT
                     SVAGLQFTLSKSSH"
BASE COUNT          902 a          571 c          516 g          780 t
ORIGIN      
        1 attcatcttt aagttcatct attgtcattt caaaactatt cgcgcgagaa ttattactat
       61 atattttacg aatgagttga tatagtgatg cagcattaac acttgtcagt cgaacaacag
      121 ataataaaac ttgcgttgta taacgttttt cttcaccaat aagtttacat aaaaatcttt
      181 tcgcttcttt tgtaaacttg atccccactc taccttcatt cctgtaataa gtagaaaaaa
      241 cagtcagact caaattcatt gaatcaggta cattactttt agtgaactta aatcccaaat
      301 cactactcag ttctaatatt tcatcatgtt tcagcttaag aagcgtcgta tctaatatat
      361 tagagccatc cctcaaagca agataagccg catcaggctt aacctgaacc catttaatgt
      421 aatcggcaac tgtcacataa aatatgtgat catcatcgaa ttcattttta gagtttatct
      481 ggcacatggc taaaaataag acttttttag cagccatagg caatgttgac agcgttgaat
      541 tcaattcatt tcgatgtctc acttttgtta agcgagaaag ggaagtctta tttgtcgtca
      601 ttctcattgt tccaacactc ttgataatca gactttaaca ccagaaacat gatattacaa
      661 tacaatcaat atcatgttta accgagtatc ttctcatgtt tcatatctgt gatttatctc
      721 acaaaggtaa tccatgctca aaatccacac tggattcaag gcagattaac aacagattca
      781 aaatagattc accagaaaga acaacgaatt agtattctta tgacacattt tgtggatatc
      841 accgaggaag ctctcatgtt taaccgagga agttatcatg tttaaccgag gaagctctca
      901 tgtttaaccg aggaagctct catgtttaac cgaggaagtt atcatgttta accgaggaag
      961 ctctcatgtt tagacaaaaa acacgcttaa aatcaataaa ttagagctac tccaagtttt
     1021 taagttttta agaaaaacaa gaaaagataa ggcttttaat gcgtgcgaat attcaggaac
     1081 tgtggataac tcaacaaaac cagcaacgat gatggttcct taccataaat ctgtcccccg
     1141 atgattatta cccgcaagct aaaacaaaca tcatcaatca ttaaaactca taactttcaa
     1201 taacataaaa tcatcaaaaa aatatttgat gccttatgaa tctcaatttt caagaccata
     1261 aatttgtccc tagttaactt taacttattg aaataaatat aatattcgtg cgtactgatc
     1321 ctaatgtcac atcgcaaaaa ttccgcttcc tcgctcactc agtcgctacg ctcctgccgt
     1381 tcggctgcgg cgagcggtat ctcctttctc aaaaccagag agattcagat catatgatcc
     1441 gttatttgat cctttttgga tcttccacta agaagccctg aatcgcgctc tgccgcttcg
     1501 ttcgatacaa ccatgaggaa aacatgcgaa atgaaaaaaa tcgcttaaaa cgcgatacag
     1561 cgcgttttag ggggtattca caaaacacac gttgccttga agaaattttg atcaagattt
     1621 aactactatc cggatcatga caacagatca tagcaacaag gctgctaaaa agcgttgaga
     1681 gacgttaaac gctgttaaga ctagggctaa gtacaatgaa acggaaaaaa tgctcagaat
     1741 gcgttacaga accttccaga aagctaaggt ttgtcacttt aaagagacat ttttcttgat
     1801 ctagtgtcac ttataagaga caatgtacac aggttaaggg aatgagggcg gcaagatgcc
     1861 ttacacggcg aaacgatatc agacagaaaa cggtgaggtt ccgtacaccg actggatgaa
     1921 aaaattaaga cgtaaagacc agaccgcagc gttaaaggtg gattccagaa ttgcccgcgc
     1981 tatgggcgga aattttggag atcataaatt cgaaagggac ggtgtatggg aattacgggt
     2041 tgattacggt cctggttaca gggtttatta ttcaatcgaa gatggcgaaa taattcttct
     2101 gctcattgga gggaataaaa aaactcagac agccgattta gataaagccg ttagttatct
     2161 gcaagatttt aaaaagaggt caaaaaaatg acagccacca aaaatgtgaa gtcgcagaaa
     2221 aatgattatc cggcatccgt tgatcacaat gccgccatga ttgaagaatt gcgcgcagat
     2281 cctgattatg caaacgctta tcttgctaat gccctggaag aaataaacga gcctggtggt
     2341 ttaggtggtt ttcttgttgc actacgtcag gtaatcgagg cgcgaggggg gatttccgaa
     2401 gctgccagga aatctggcct tgcccgtcag agtatttacc gcgcattatc ccccaacggt
     2461 aacccgacca tcacaacgct tgcacaactt acatcggtag ccggattaca gttcactctc
     2521 agtaaatcca gccactaatc tctccagttc cccctaagcg ggggaatttc catgccatca
     2581 aaaccgcagt tatcccgccc gacgttcacc ggtatttaat cggcaaatcg tacaattgcg
     2641 tactggcaat agtttaacgg tgtgtattaa tacacattta aagacgatgt gatagatgcg
     2701 tgtgttaatg ctaatgtatg catacattag cattaacata gagcagattc tggcgcgttg
     2761 cgcccgtag
//
LOCUS       DAZFFE010000225         2752 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFFE010000225 DAZFFE010000000
VERSION     DAZFFE010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.227"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(24..683)
                     /locus_tag="ACHJ69_004837"
     CDS             complement(24..683)
                     /locus_tag="ACHJ69_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5017623.1"
                     /translation="MIKIENLTKSYRTPTGRHYVFKNLNIIFPKGYNIALIGQNGAGK
                     STLLRIIGGIDRPDSGNIITEHKISWPVGLAGGFQGSLTGRENVKFVARLYAKRDELN
                     ERVDFVEEFSELGKYFDMPIKTYSSGMRSRLAFGLSMAFKFDYYLIDEITAVGDAKFK
                     KKCSDIFDKIREKSHLIMVSHSERALKEYCDVAIYLNKEGQGKFYKNVTEAIADYKKD
                     L"
     gene            complement(680..1456)
                     /locus_tag="ACHJ69_004838"
     CDS             complement(680..1456)
                     /locus_tag="ACHJ69_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5017624.1"
                     /translation="MARSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPSAHLL
                     ILLGILGYVMHRTMPDISFPVFLLNGLIPFFIFSSISKRSIGAIEANQGLFNYRPVKP
                     IDTIIARALLETLIYVAVYILLMLIVWMTGEYFEITNFLQLVLTWSLLIILSCGVGLI
                     FMVVGKTFPEMQKVLPILLKPLYFISCIMFPLHSIPKQYWSYLLWNPLVHVVELSREA
                     VMPGYISEGVSLNYLAMFTLVTLFIGLALYRTREEAMLTS"
     gene            complement(2630..>2752)
                     /gene="gspM"
                     /locus_tag="ACHJ69_004839"
     CDS             complement(2630..>2752)
                     /gene="gspM"
                     /locus_tag="ACHJ69_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFO5017625.1"
                     /translation="LLNWLNALDEKYALRVTQIDVSAAEKPGMVNVQRLEFGRG"
BASE COUNT          859 a          588 c          469 g          836 t
ORIGIN      
        1 ggcataatat tggaatgtaa ccactatagg tcttttttgt aatcagcaat ggcttccgta
       61 acatttttat aaaatttacc ttgcccctct ttgttaagat aaatagcaac atcacaatac
      121 tcctttaaag ctcgttcact atgtgacacc attattaaat gagatttttc tcttatttta
      181 tcgaatatat ctgaacattt ctttttaaac tttgcgtctc caacagcagt gatttcatca
      241 ataagataat agtcgaattt aaaagccata cttaatccaa aagctaacct tgacctcatg
      301 ccagaagaat aagttttaat gggcatatcg aaatattttc cgagttcgga aaactcttca
      361 acaaaatcaa ccctctcatt taactcatcg cgttttgcat atagtcgggc aacaaattta
      421 acattttcac gaccggttaa acttccttga aatccaccag ccaatccaac gggccatgaa
      481 attttatgtt ctgtaataat gttcccacta tcaggacgat ctataccacc aattatccga
      541 agtaaagttg atttaccggc tccattttga ccaatcaggg caatgttata gcctttagga
      601 aaaataatat tcaaattttt aaaaacataa tgccgcccag tgggcgtgcg ataagacttc
      661 gtcaaattct caatcttaat catgatgtca gcattgcctc ttcacgcgtt cggtataatg
      721 ccaggccgat gaacagggtg accagagtaa acattgcaag gtagttcaga ctcacgcctt
      781 cactgatata gccaggcata actgcctcgc ggcttaactc cacaacatgc actaatgggt
      841 tccagagtag atatgaccag tattgctttg gaatcgagtg tagagggaac atgatgcagg
      901 agatgaaata cagcggctta agcagtatcg gcaggacctt ttgcatttca ggaaaggttt
      961 taccaacgac cataaatatt aagccgacgc cacatgaaag aatgattaac aaactccagg
     1021 tgagcacaag ttgtaaaaag tttgtaattt cgaaatattc gcctgtcatc cagacgataa
     1081 gcatgagcaa aatataaaca gcaacgtaaa tcagtgtctc aagcagtgca cgtgcaatga
     1141 tcgtatcgat gggttttact ggtcgataat taaacagtcc ctggttcgct tcaatagcac
     1201 caatagaacg tttgctaata ctactaaaga taaaaaaggg aatcaggcca ttaagtaaaa
     1261 acaccgggaa cgagatgtct ggcatagtgc ggtgcataac gtaacccaaa attcccaaca
     1321 atatcagcaa atgcgcggag ggttcaagaa tcgcccacaa ataccccaga cgaaacttac
     1381 caaagcgtgt tcgtatttct cgtagaaata atgcctctac ggtgactttc tgaacttcaa
     1441 atccacttct tgccatttga tgatgtgatc ctaatctctt caggtacgct accgcccttg
     1501 gcttaacagc taccaataca ctggaggtaa tacttgtttg taactggata aaatttactc
     1561 agacggtttg acctttttaa tagccgcaaa aaaatttcag cagaaattat ttccgcaggg
     1621 ataaatagat ctatttccgt ctcgtttctt ctaatccatt cttcaggcat gaatgtattc
     1681 ggtcttctgg taatttcaga aaactggccg ttttagaatg tagagttaat ttctcaggaa
     1741 attatttcca cctctttttg cttaaccaat atggcttgaa aaagacgtaa agcagcatgc
     1801 gttctggatt actccggaac acacttattc aaaatgggct gctaatttcg caagaaatta
     1861 ttttttacta ttactgttaa ctacttgatt ataataccac ctatgaattt tatgcgtaac
     1921 aacacctgca atctgtctca aaccagaacg cacgcgattg attaccaata aattgtttag
     1981 tgagaaacta tttccctatt taaaattcac tcatgtactt cttatttata tctacagccc
     2041 cctctttaca gtcacatttg tgatttacat cacatttaaa cggtgttatt taagccccat
     2101 taataattcc gtgggtaatt attttcgtca attaaaatat acatcttgat tattttgtgt
     2161 atttatttta accagtgtta caaccaacac catccctcag tagatatacc tgagcagaca
     2221 tttcttaaaa tcgacgaata gtttccctgg gaattattat gcttatttga aattcccttt
     2281 ctgattatct ccagtaattt tactaatttc caggctacat aaaatacaga tcacataaga
     2341 attatcctat atgttttgac ctatcaattg ttactcaatg atttattaca actattaata
     2401 atcataatgt gtcaaaacac ttaagcatct ttacagtctt agaaagatgt cgattaacga
     2461 aggctacatg gtgaaaaaca ggttccttca gtgttaccga atattccgtg gtggtctgga
     2521 gggagataat tcttgctaca tcgaaactca gcccggtgag cgggtttgac agttccgcac
     2581 tcaccattgg gctaagggtt atcaggtggg gttaaggaaa tggcaatacc tacccccgcc
     2641 caaactccag tcgctgcaca ttcaccatcc caggcttctc agcagcactg acatcaattt
     2701 gtgtcacccg cagcgcatat ttttcatcca gtgcgtttaa ccagttcagc ag
//
LOCUS       DAZFFE010000226         2740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZFFE010000226 DAZFFE010000000
VERSION     DAZFFE010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.228"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..465
                     /locus_tag="ACHJ69_004840"
     CDS             <1..465
                     /locus_tag="ACHJ69_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO5017626.1"
                     /translation="GKKLKKDNPNYNGDLGFKIFETVHDFRAKDESELTLSNLSFFDD
                     AVLTPEQYDTLLTTWCVYDGSLLTTPIEDIDLNGYKAHLCDGRLYLIAPNFTSEALKA
                     LLQKLDADKDFAPNKVVFYGSNFESAKQMELNEALQSYANKKSIDLDLVVRN"
     gene            468..>2740
                     /locus_tag="ACHJ69_004841"
     CDS             468..>2740
                     /locus_tag="ACHJ69_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFO5017627.1"
                     /translation="MSKGFTFEKNLPHQKAGVDSVMNVFVSAIPHQTDHVAIRLLANP
                     ELNLSEQQYYNNIKNVQEFNGIEHSKDNYDAKSNVIDVSMETGTGKTYTYTKTIFDLN
                     KSFGINKFIIIVPTLSIKAGTVNFLKSDALKEHFRDDYGRELKTYVVESQKSSGKNTK
                     SYMPQAIHDFVEASNFNKKYIHVLVINSGMINSKSLTDTYDVGLLDNQFDTPFAALSA
                     VKPFIIIDEPHKFPTGKKTWENIEKFNAQYIIRYGATFSEGYKNLVYRLTAVDAFNED
                     LVKGIDAYIEDIVGDGNANLKFVKSDGKEATFELNENSNKKTFKLVKGESLSKTHSAI
                     HDLTLDALNKSTAVLSNGIELKIGSSINPYSYDQTLADNMMRKAVKEHFKLEKEYLTQ
                     RPRIKPLTLFFIDDIEGYRDGNDIAGSLKTKFEEYVLAEANELLKIEKDEFYRNYLEK
                     TIKDISSVHGGYFSKDNSDKDDKIEQEINEILHDKELLLSLENPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNEYMCRVKDRNFTLNYYVDFTEKDFV
                     DSLVKEINDSSFKETVPSKFTQELKDKILSQYPELSSRTLMNEIFDDEIIDDNDNFKD
                     SDAYSRLKAKYPAAFPTGVKPSKIKKATDGKRRTKMRVGKFSELKELWDLINQKAVIE
                     YKINSENEFLSIFKSFMLEETERFTKSGVHTRIDKIYIYNDTAMSKSIISGDDNFAKL
                     NTMSYREFLDNLSQTIFA"
BASE COUNT          962 a          455 c          511 g          812 t
ORIGIN      
        1 ggaaaaaagc tgaaaaaaga taatcctaat tataatggag atttgggatt taaaatattt
       61 gaaactgttc atgattttcg agcaaaagat gagtctgaac ttacgctttc taatcttagt
      121 ttctttgacg atgctgtatt gacaccagag cagtatgaca ctctattgac gacatggtgc
      181 gtgtatgatg gtagcttgct aacaacacca attgaagata ttgatcttaa tggctacaaa
      241 gcacacttgt gcgatggtcg gctatatcta attgcgccta atttcacaag tgaggctttg
      301 aaagctttac ttcaaaagct ggatgcagac aaagactttg cccctaacaa agttgtattt
      361 tatggcagca acttcgaaag tgcgaaacag atggaactca atgaagcact acaaagttat
      421 gctaataaaa aatctattga tttagatttg gtggtaagga actaatcatg tctaaagggt
      481 tcacattcga aaaaaattta cctcatcaaa aggctggcgt tgattcggta atgaatgtct
      541 ttgtatccgc catacctcat cagacggatc atgttgcaat tcgcctgtta gctaatccag
      601 aactgaatct atctgaacaa caatactaca acaacataaa aaatgttcag gagttcaatg
      661 gtattgagca ctcaaaagat aactacgatg ctaaaagcaa cgtaattgat gtttctatgg
      721 agacaggtac aggtaaaact tatacttaca ccaaaacaat ttttgaccta aacaaatcat
      781 ttggcattaa caagtttatt atcattgttc cgactctatc gatcaaagcc ggaaccgtta
      841 actttttaaa aagtgatgct ttaaaagaac actttcgaga tgattacggg cgtgaactaa
      901 agacttacgt tgttgaaagt cagaaaagtt ctggtaaaaa tacaaaatca tatatgcctc
      961 aagctattca tgatttcgta gaagcaagta attttaataa gaaatatatt cacgttcttg
     1021 tgatcaactc agggatgatt aactcaaagt ctttaactga cacttatgat gttggtttgc
     1081 ttgataatca atttgatacg ccatttgctg ctcttagtgc ggttaaacca ttcattatca
     1141 ttgatgagcc tcataagttt cctactggta aaaagacatg ggaaaatatt gaaaaattta
     1201 atgctcaata tatcatccgc tatggagcaa cctttagtga aggctataag aacttagttt
     1261 atcgtcttac agccgtagat gcattcaatg aagatcttgt taaaggtatt gatgcataca
     1321 ttgaggatat tgttggtgat ggtaatgcca acctcaagtt cgtcaaatct gacggaaaag
     1381 aagctacatt tgaactgaac gaaaatagca ataaaaaaac atttaagtta gtcaaaggtg
     1441 aatcactatc taaaacgcat agtgcgatcc atgatttaac actggatgcc ttaaacaaga
     1501 gcactgctgt attgagtaat ggtattgaac taaaaattgg tagttcaata aacccctact
     1561 cttacgatca gacacttgct gataacatga tgcgtaaagc tgtcaaagaa cactttaagt
     1621 tagaaaaaga gtatctgaca caaaggccac gaataaagcc tcttactctt ttcttcattg
     1681 atgatattga agggtatcgt gatggtaatg atattgccgg aagtctgaaa accaagtttg
     1741 aagaatacgt attggcagaa gctaatgaac tgttaaaaat agagaaagat gagttttata
     1801 ggaactacct tgaaaaaact attaaggata tatcttctgt tcatggtggt tatttttcaa
     1861 aagataatag tgataaagat gataaaattg agcaggaaat caacgaaatc cttcatgaca
     1921 aagagctttt attgtcttta gaaaatcctc gccgtttcat tttctctaaa tggacgttgc
     1981 gtgaagggtg ggataaccca aatgtttttc agatctgtaa gctccgttcg agcggtagca
     2041 ccacatctaa acttcaagag gtagggcgtg gtttacgtct tccagtgaat gaatacatgt
     2101 gtcgtgtgaa agaccgcaat tttaccctca actattatgt tgattttaca gaaaaagatt
     2161 ttgtcgattc tcttgtcaaa gagatcaacg atagttcttt caaagagact gttccaagta
     2221 agtttactca agagcttaag gataaaatcc tatctcaata tccagagctt tcatccagaa
     2281 cactcatgaa tgaaattttt gatgatgaga tcatagatga taatgataac ttcaaagatt
     2341 cagatgccta tagccgctta aaagccaaat atccggcagc atttcctaca ggagtgaaac
     2401 caagcaaaat caaaaaagcc actgatggaa aaagacgcac taaaatgcgt gttggtaagt
     2461 tcagtgagct gaaagaacta tgggatttga tcaatcaaaa agcagttatt gagtataaaa
     2521 taaatagcga aaatgaattc ttatccattt tcaagtcatt catgctcgaa gaaacagaaa
     2581 gatttactaa atcaggcgtt catactcgca ttgataaaat ttatatctat aatgatacgg
     2641 caatgtcgaa aagcattatc agtggtgatg ataactttgc taaactaaac acaatgagct
     2701 atcgggaatt tcttgataac ttatcacaaa ctatttttgc
//
LOCUS       DAZFFE010000227         2572 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFFE010000227 DAZFFE010000000
VERSION     DAZFFE010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.229"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1549
                     /locus_tag="ACHJ69_004842"
     CDS             <1..1549
                     /locus_tag="ACHJ69_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO5017628.1"
                     /translation="AEVADSVVRDCLVSRAIYSDVPEHHLLNVMAYFQAISGFDFAPV
                     YATYHDRYGDEGLLKRLTDLAFVTRFLRVFRDQRVNEVCRMLGILNRNAPYISDWHRD
                     LFAVRSKRVKKYLRSSGVFDAFNELVCTLEDAHNASVSNPKNRIAELCVRGKAVCELS
                     EDMGLSGYFIVLTTPSRFHPTTSFKVAGKWHSRPNKKWWEAGCPTVKDSHAWLNTVWR
                     RVCRRLDKAGIQIPGLRTVEPHADGTTHWNFLIYCNPHESATVLAIFREEAMRDEPDE
                     KGAKEHRIRIEAIDPEKGDGFRYIVKYITKMAGDVSADGITALNDRYSARSFCDAVSR
                     AACWQKATRLRLFQFFGVPSVTAYRQMRTFRSPLEAHHINMQQFTPQQVAELEAIRMA
                     CDAGDFRTYILLNGGFFCSERLLRPFYVQPQEGGKPRFNRYGEPCAPVISGFMFGPVP
                     VITRFMGCVVRRMTPAEKIRAEEVRSGSSYESVFISSASRHRTTRSRAAGGGGEADPW
                     TCDNNCP"
     gene            1580..1813
                     /locus_tag="ACHJ69_004843"
     CDS             1580..1813
                     /locus_tag="ACHJ69_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017629.1"
                     /translation="MNQIYDSFPFPSPEGFTSGMTMAGYFIHVTVINDEELKPTGSSV
                     IGIAGTAAFAVLGVSLAARLGIGIIKGFLSRAT"
     gene            1874..>2572
                     /locus_tag="ACHJ69_004844"
     CDS             1874..>2572
                     /locus_tag="ACHJ69_004844"
                     /inference="COORDINATES: protein motif:HMM:NF017195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017630.1"
                     /translation="MIHFIIPLLFLFFSSFSFAEEKENPDALVQCAGHSSCRTDYSDA
                     GTSIKLARAAGHLFCGSGSYDACSGIIHYCGVPLSTPVNAPSTVGSFVRARWSAYKVW
                     DSDKQKWTACTLAFQGGEGDLTPEYNKPPTDEICLSRPVLDGMTFSDVYKGDDGTRYV
                     NVQGCIYEATGNPLICNPDDDTVCTADWKPVAVDPAFSDGKEDGGSSGGGNPGGGSSG
                     GGSGSGSGGSSGSGG"
BASE COUNT          510 a          641 c          693 g          728 t
ORIGIN      
        1 tgctgaggtg gctgattcgg ttgttcgtga ctgccttgtt tctcgggcca tttatagtga
       61 tgtaccagag catcaccttc tcaatgtgat ggcctatttt caggccatct ccggctttga
      121 tttcgccccc gtttatgcca cttatcatga tcgttacggc gatgagggat tgcttaagcg
      181 tctgactgat ttggcttttg tcacccgttt cctgcgggtt ttccgtgacc agcgggtcaa
      241 tgaggtctgt cgcatgctcg gcatactgaa ccgcaacgcg ccttatattt ccgactggca
      301 ccgcgacctc tttgctgtcc gcagtaaacg cgtgaaaaag tatctccggt cctccggcgt
      361 ctttgatgcc tttaatgagc tggtctgtac gctggaagat gcgcataacg cctctgtttc
      421 gaatccgaaa aaccggattg ctgaactctg cgttcgtgga aaggccgtct gtgaactgtc
      481 cgaagatatg gggctgtccg gctacttcat cgtgctcacc acgccgtcac gttttcaccc
      541 gacgaccagt tttaaggttg ccggaaaatg gcattctcgt ccgaataaaa aatggtggga
      601 agcaggttgc ccgaccgtta aggattcgca cgcctggctg aataccgtct ggcgtcgggt
      661 ctgccgcaga ctggataaag ccggtattca gatacccggt ctgcgcacgg tcgagcctca
      721 tgctgacggt acaacgcact ggaatttcct gatttactgc aatccccatg agagcgcaac
      781 ggtgctggcc attttccgtg aagaggccat gcgcgatgag ccggatgaaa agggggcgaa
      841 agagcaccgt attcgtattg aggctattga ccctgaaaag ggtgatggtt tccgttacat
      901 cgtgaagtac atcaccaaaa tggcgggtga tgtcagtgcg gatggtatta ccgccctgaa
      961 tgaccgttat tctgcccgtt cattctgtga tgccgtcagc cgtgccgcct gctggcagaa
     1021 agcaacgcgc cttcgcctgt tccagttctt tggcgttccg tccgtcacgg cttaccgcca
     1081 gatgcgtact tttcgttcac cgcttgaagc acatcatatc aatatgcagc agttcacgcc
     1141 gcagcaggtc gccgaactgg aggccatccg catggcctgt gatgcaggcg atttccggac
     1201 ctatatcctg ctgaacggcg gtttcttctg ttctgaacgt ctgcttcgtc cgttttacgt
     1261 tcagccgcag gaaggtggta aaccccgttt taaccgttac ggtgaaccct gcgcaccggt
     1321 gatttccggt ttcatgtttg gccctgttcc ggtcataacc cgttttatgg gctgtgttgt
     1381 ccgccgtatg acccccgcag aaaaaatccg cgcagaggaa gtcagaagcg gcagcagcta
     1441 tgaatctgtg tttatttcgt cggcttcacg acaccgcacg acgcgctccc gcgcggcggg
     1501 tgggggcggc gaagccgacc cttggacttg tgacaataac tgtccctgac tgaattttta
     1561 acattgagga gtctgattta tgaatcaaat ttacgacagt tttccgtttc cttcccctga
     1621 gggttttacc tcaggcatga caatggcggg atattttatt cacgtcactg tcataaatga
     1681 tgaggagtta aaacccacgg gttccagtgt gattggcatt gcaggtacgg cggcatttgc
     1741 cgttcttggt gtttcactcg ctgcccgtct gggtatcggt atcattaaag gcttcctttc
     1801 ccgcgcaacc tgatagttat ttaacgacag cgccctccgg ggcgcttttt taatcatacc
     1861 aggagtgttt tgtttgattc actttattat tccgcttctg tttttgttct tttcctcgtt
     1921 ttctttcgct gaagagaaag aaaatccgga tgcacttgtt cagtgtgcag gccattcatc
     1981 ctgcaggaca gattattcgg acgcggggac gtcgattaaa ttagccaggg cggccggtca
     2041 tcttttctgt ggttcgggca gttatgacgc atgttccgga ataattcatt attgtggtgt
     2101 gccactttcc acgcctgtta atgcaccttc gactgtcggg tcttttgtcc gtgcccggtg
     2161 gtcagcatat aaagtctggg attcagacaa gcaaaagtgg acagcctgca cccttgcttt
     2221 tcagggtggt gagggggatt tgacacctga atataataag ccgccaactg acgagatttg
     2281 tctttctcgt ccggttcttg atggtatgac tttttctgat gtttataaag gtgatgacgg
     2341 gacccgttat gttaatgtgc aggggtgtat ttatgaggca accggcaatc ctcttatttg
     2401 taatcccgat gatgatacag tttgtactgc cgactggaaa ccagttgctg tagatcctgc
     2461 attctccgat ggtaaggagg atggcggtag ctcaggtggt ggtaacccag gcggcggcag
     2521 ttccggtggt ggttcaggtt ctggctccgg tggttcatcc ggtagcggtg gc
//
LOCUS       DAZFFE010000228         2546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFFE010000228 DAZFFE010000000
VERSION     DAZFFE010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.230"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            16..969
                     /gene="ompT"
                     /locus_tag="ACHJ69_004845"
     CDS             16..969
                     /gene="ompT"
                     /locus_tag="ACHJ69_004845"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO5017631.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKIPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1201..1869
                     /locus_tag="ACHJ69_004846"
     CDS             1201..1869
                     /locus_tag="ACHJ69_004846"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5017632.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFDPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVKGTVVMFSA
                     SGKK"
     gene            complement(1924..2293)
                     /locus_tag="ACHJ69_004847"
                     /pseudo
     CDS             complement(1924..2293)
                     /locus_tag="ACHJ69_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2300..2546
                     /locus_tag="ACHJ69_004848"
                     /pseudo
     CDS             2300..2546
                     /locus_tag="ACHJ69_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          773 a          568 c          550 g          655 t
ORIGIN      
        1 gaatggagac cttttatgcg ggcgaaactt ctgggaatag tcctgacaac ccctattgcg
       61 atcagctctt ttgcttctac cgagacttta tcgtttactc ctgacaacat aaatgcggac
      121 attagtcttg gaactctgag cggaaaaaca aaagagcgtg tttatctggc cgaagaagga
      181 ggccgaaaag tcagtcaact tgactggaaa ttcaataacg ctgcaattat taaaggggca
      241 attaattggg atttgatgcc ccagatatct atcggggctg ctggctggac aacactcggc
      301 agccgaggtg gcaatatggt cgatcaggac tggatggatt ccagtaaccc cggaacctgg
      361 acggatgaaa gtagacaccc tgatacacaa ctcaattatg ccaacgaatt tgatctgaat
      421 atcaaaggct ggctcctcaa cgaacccaat taccgcctgg gactcatggc cggatatcag
      481 gaaagccgtt atagctttac agccagaggt ggttcctata tctacagttc tgaggaggga
      541 ttcagagatg atatcggctc cttcccgaat ggagaaagag caatcggcta caaacaacgt
      601 tttaaaatac cctacattgg cttgactgga agttatcgtt atgaagattt tgagctaggt
      661 ggcacattta aatacagcgg ctgggtggaa gcatctgata acgatgagca ctatgaccca
      721 ggaaaaagaa tcacttatcg cagtaaagtc aaagaccaaa attactattc tgttgcagtc
      781 aatgcaggtt attacgtaac acctaacgca aaagtttatg ttgaaggcgc atggaatcgg
      841 gttacgaata aaaaaggtaa tacttcactt tatgatcaca atgataacac ttctgactac
      901 agcaaaaatg gtgcaggcat agaaaactat aacttcatca ctactgctgg tcttaagtac
      961 accttttaac aacgttaaat agattttctc cccggtggta ttgtatagcc ccggggaatt
     1021 tgtttaccgt taaaagcaaa caatatcccc taatcttgta agcatttaac ctgcgtcctg
     1081 gcgtgtaacc tcttaccgca acacaagact caatatattc aaattcgtta tgccgtggca
     1141 tcatttaacc ccttgtaatt catcgctata attgatttaa ttcacaaata aaactataac
     1201 atggtgaaac taatgaaaaa aaacacagat gatggggcta aaatttacac accacttacc
     1261 ctaaagcttt atgactggtg ggttttgggt gtatcaaatc ggcttgcatg gggatgtcct
     1321 acaaaggaac accttcttcc acactttctg gaacatttag gtaacaacca tctggatatt
     1381 ggcgttggaa ctgggtttta ccttactcac gtacctgaga gtagtctgat atctttaatg
     1441 gatttgaacg aagctagcct gaacgcggca tcgacaaggg ctggggaatc aaaaattaaa
     1501 cataaaatta gccatgatgt ttttgatcct tatcccgcgg cgttacatgg tcaatttgat
     1561 tccatttcca tgttttacct tcttcactgc ctgcctggaa atatatctac aaaaagctgt
     1621 gtaatacgca atgcggcgca ggccttaact gacgatggaa ctctatacgg agccacaatt
     1681 cttggtgacg gcgttgtgca caatagcttc ggtcaaaaac tgatgcgcat ttacaatcag
     1741 aaaggcatct tttcaaacac aaaagattcc gaagaaggct taacacatat actctcagag
     1801 catttcgaga atgttaaaac caaggttaaa ggtactgtag taatgttttc cgcttcaggg
     1861 aaaaaatagc atccagccgc agcacgttct tgcgcacgac gtgctgcggc ataatcccaa
     1921 tgattactct ctgacagggt tcacaggcca ctcaatatca ggggcagttg atgtatcaac
     1981 tcggttcagc aacacccgat acatcttcca ggcttccagc aacgaggttt cttcctccgt
     2041 atcctttatc catgatgtgc cgttctactt ctgaaactcc cctgccgggg ataccaggtg
     2101 acattttccg gtaacggacc gagttcagaa ataaataatg cgtcccctga tgccacgtca
     2161 taaaccgttt taccccgatg gtcttcaacg agatgccacg atgcctcatc actgttgaaa
     2221 acaaccacga agccagccgg aatatctggg caatatcagt actgtttgca ggcaaacctg
     2281 tatgaggtgg aatggcacaa gagacttaca aaagaatcag gcaaaacgcc tgattgatga
     2341 gagccccgaa ctactgaaat ctgtcgtcaa atttgcgctg gtaactgttc tgagaaagtc
     2401 gaacatcata aatctggagt ggcaacagat tgacatgcag cgacaagttg ccagggtgaa
     2461 tccagaagat agcaaatcaa accgcgccat tggtgtggcg ctgaacgata ccgcctctaa
     2521 agtgttgcgt gatcaaacag gcaagc
//
LOCUS       DAZFFE010000229         2508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFFE010000229 DAZFFE010000000
VERSION     DAZFFE010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.231"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..66
                     /locus_tag="ACHJ69_004849"
     CDS             <1..66
                     /locus_tag="ACHJ69_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFO5017633.1"
                     /translation="DAFCRTLTRGSLAIPQCHLLS"
     gene            200..746
                     /gene="cobU"
                     /locus_tag="ACHJ69_004850"
                     /pseudo
     CDS             200..746
                     /gene="cobU"
                     /locus_tag="ACHJ69_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
     gene            743..1486
                     /gene="cobS"
                     /locus_tag="ACHJ69_004851"
     CDS             743..1486
                     /gene="cobS"
                     /locus_tag="ACHJ69_004851"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO5017634.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELLFLLALL"
     gene            1498..>2508
                     /gene="cobT"
                     /locus_tag="ACHJ69_004852"
     CDS             1498..>2508
                     /gene="cobT"
                     /locus_tag="ACHJ69_004852"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO5017635.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYN"
BASE COUNT          543 a          550 c          754 g          661 t
ORIGIN      
        1 gacgcatttt gtcggacgtt aacacgagga agtctggcta ttccacaatg ccatcttctt
       61 tcgtaatgga ttgagaatgc tggattttta attatattac gcgcagtatt gatcgtgtaa
      121 atgttttgcg gtcagcaatg gtgggggatc cattgttctg ttcaatgaat accatcatac
      181 aattgaatca ggagtcggta tgatgatttt ggtgacgggg gggcgcacgg agcgggaaga
      241 gtcgccacgc agaggcgctt attggggact cttcacaggt tctgtatatc gccacctcgc
      301 aaatccttga tgacgagatg gctgcacgga tagaacatca tcggcaaggc cgtccggcgc
      361 actggcgcac ggtggagcgt tggcaacatc ttgatgaatt aattcatgca gacattaacc
      421 cgcatgaagc tgtgttgctg gaatgcgtta ccacaatggt gactaatctg ttgtttgatt
      481 atggcggcga taaagaccct gatgaatggg attatcaggc gatggaacag gcgattaatg
      541 ctgagattca gtcgttgatt gctgcctgcc aacgttgccc cgcaaaggtt gtattagtga
      601 ctaacgaagt gggaatgggg attgtgccgg agagtcgtct ggcacgacat tttcgtgata
      661 ttgccgggcg ggtaaatcag cagctggccg ctgcggcaaa tgaagtatgg ctggtggttt
      721 cgggtattgg agtaaaaatc aaatgagtaa attattttgg gcgatgctct catttattac
      781 gcgtctgcct gtaccgcgtc gctggtccca gggactggat ttcgagcatt attctcgcgg
      841 tattattact tttcctttaa ttggattatt gcttggcgcg attagcgggc tggtcttcat
      901 ggtgctgcag gcatggtgtg gcgtaccact ggcggcactg tttagcgtac ttgtgctggc
      961 gctgatgacc gggggattcc acctagacgg cctcgccgat acctgtgatg gcgtattttc
     1021 tgcacgtagc cgcgatcgca tgttggaaat catgcgtgat agccgtttag gcacccatgg
     1081 cggtctggca ttgatttttg ttgtactggc aaagattctg gtattaagcg agttggctct
     1141 gcgtggcgaa ccgatccttg catcgctggc ggcagcatgt gcggtcagcc gcgggactgc
     1201 cgcattattg atgtatcgtc atcgttatgc ccgggaagaa gggcttggca atgtgttcat
     1261 tggcaaaatt gatgggcgac aaacctgcgt cacactcggc ttagccgcta tttttgctgc
     1321 cgtattattg ccgggtatgc atggtgtggc tgctatggtg gtgacgatgg tggcgatttt
     1381 catcctcggt cagcttctca aacgtacgct gggcgggcaa accggcgata cgctgggagc
     1441 tgctatagag cttggtgaac tgctcttcct gctggcactg ctataaagag acgtgacatg
     1501 caaacacttg ccgatttact gaatacgatc cctgctatcg atcctgccgc tatgtcgcgt
     1561 gcacaacggc atattgacgg gttactcaaa cctgttggta gcctgggaag gctggaggcg
     1621 cttgccatac aactggcggg aatgccgggg ttgaatggca taccgcatgt gggcaaaaaa
     1681 gcggtactgg ttatgtgtgc cgatcacggc gtctgggagg aaggggtcgc tatttcccca
     1741 aaagaagtga cagccattca ggctgaaaat atgacccgcg gaacaaccgg cgtgtgtgtg
     1801 ctggcagcac aagcgggcgc taacgtccac gtagttgatg ttggtattga tagtgctgag
     1861 cctatccccg ggcttatcaa catgcgtgtc gcacgaggta gcggcaatat tgcttcagct
     1921 ccggcaatga gtcgccgtca ggctgaaaag ttgcttttgg acgtcatatg ttatacgcgg
     1981 gagctggcaa aaaacggtgt cacgctgttt ggtgtaggtg aactggggat ggcaaacacg
     2041 acaccggcag cggcaatagt cagcacaatc actggccggg atcctgaaga agtggttggg
     2101 attggcgcaa acctgccgac agataaactg gctaataaaa ttgatgttgt gcgccgggcg
     2161 attacgttga atcaaccaaa tccgcaggat ggcgttgatg ttctggcaaa agtgggggga
     2221 tttgatttgg tcggaatggc tggcgtgatg ttaggcgctg cttcctgcgg tttacccgtg
     2281 ttgctggatg gatttctttc ttatgctgct gcgctcgcag cctgccagat gtctcctgca
     2341 atcaaaccgt atctcattcc ttctcacctg tcggcagaaa aaggggcgcg tatagcgctg
     2401 tcgcatttgg ggctggagcc ttatctcaat atggagatgc gtttaggtga ggggagtggc
     2461 gcagctctgg cgatgcccat catcgaagct gcctgtgcga tatataac
//
LOCUS       DAZFFE010000230         2489 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZFFE010000230 DAZFFE010000000
VERSION     DAZFFE010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.232"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(157..2424)
                     /gene="adiA"
                     /locus_tag="ACHJ69_004853"
     CDS             complement(157..2424)
                     /gene="adiA"
                     /locus_tag="ACHJ69_004853"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO5017636.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANIGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT          570 a          613 c          677 g          629 t
ORIGIN      
        1 taaataacag gcaaaaaaaa gcgcggggtg ggaccgcgca aaaagcatga cattttttgt
       61 ttgcagcaat ttttatgaaa acgttgccat acttaaaagg cctcaagcca ggcggcgtaa
      121 tgttatttaa caattacgcc ttcagcggaa tagtggttac gctttcacac acataacgtg
      181 gtaaataccg tcaataattt ctgtcccttc tgtttcgtgt tcaaatccag ggaaatgatg
      241 gtcccaggat tgcagcgagc gtaaataact tacttgcgga ctgtttttat cgccgaagtt
      301 ttcaccagac agcagcatcg ggattcctgg cggatacggg ataactgagt ttgccgcgat
      361 acgtcctggc agattttcaa tggataccag ttcgacattg ttgtcgacaa tcgcgttgta
      421 cgcttcacgc ggggtgattt ccgccaccgg taggccggaa taggcttcat tcaaccgtgc
      481 gccagggttg ttttctttca gccaggcaaa catggtgtca cccagatcgt gaatccctat
      541 gttcgcgtaa gtgtcaggat attgttcaac aagttccggc atcacctgcg ccagcggtgt
      601 gttggcatca tagtggcgtt tgaaggagca aagggtgtta accagagtcc cccatttccc
      661 acgggtcacg cccatagaga acaggaacat aatctggaag tcagtggtac gggtaggcac
      721 aatgccgtgg cgaccaagcc aggctgtgac cagcgccgcc ggaacaccgg tttcttccag
      781 ttcaccatct tcacccattc ccggagcaag gatgctgact ttaattgggt cgagcatact
      841 ccagttatcc ggaatatctt tgaagccgtg ccagctttcg cccggatgca tgacccagca
      901 gtcctgaacg gtggtcagca gtttggttgg tgcgtcagca aagtcatagg ttttgccggt
      961 ttgtgggtcg gtgacgactt ctttgttcca cggtttgaag aaccagctac cgtcagcggt
     1021 gaactcttta tatagccgcg ccatcgcctg acggaaatca accgcttcgt cgatcacttc
     1081 ctgggtcagt gacaggccgc tgttgccgtc catcatcgac actgccacgt cgttggatgc
     1141 acagatggca tacagcgggg aggtggtggc atgcatcatg taggcctggt tgaagcggga
     1201 gaagttaatc gccccacgac cttcacgtac atgaatataa gaggcctgtg acagcgcatt
     1261 cagcagtttg tgggtggagt gggtggcgaa aacggtagga ccgttgtgat cgccaggttc
     1321 gccgcgcatg gcatagtgat cggcatagat cgggttgaaa cgtgcatagc cgtaccaggc
     1381 ttcgtcaaag tgcagacgat cggaggtttt ttccagcaga tcctgtgctt ctttagcgtt
     1441 ataacacacg ccgtcatagg tgcagttggt caccacgcag taagacggtt tttgcccggc
     1501 tttgtctttg gtcagcgggc tttcactgat tttcttctgc aaggtttcag gttgcatttc
     1561 ctgcggatat attggcccga taatgccgta gcggttgcgg cttggcacca tgtagaccgg
     1621 tttcgcgcct gtcagcatca agccttgttc gatggattta tggcagttac ggtcaacgac
     1681 cactacatcg ttatcggtca tgcaagcctg catgatggtg cggttagagc cggaagtacc
     1741 gacgactacc gaccaggagc gatcggcacc aaatacgcgt gcggcatatt tttcgctttc
     1801 gccaaatgcg ccagtatggt caagcaaaga accgagggaa gtgcgttcga tgcccatgtc
     1861 ggtgcggaac agattttcac catagtagtc atggtagaaa cgtccggcgg gtgttttagt
     1921 aaaaccaacg ccgccctggt ggcctggcgc tgcccaggaa tattcatgga tgtcgctata
     1981 tttcatcagc gcgctgaaca gtggcggcaa cagctgctgg cggtagcggg tcatcgcggc
     2041 aacggcgcgt ccggcgataa agtcggcagt atcttccaga atccaggcga attcatcgac
     2101 aagctccagc agatcccggt ccattgcggc gagggctttt tcccgatcgc ccaacaggaa
     2161 gaccggcacg ttttgttggc gctcatgaag cttaccgatc aattgtctga cgttttgatg
     2221 ttcgtccgga tgttccattt gatagctgaa catcaggcag tcaatggctt cgtttgaaga
     2281 gagaatggca aaaccatcat caaaggaggt ggatttaatc acggtaacat tttgctggct
     2341 taaagcatct gccagacgct caacggcgtt accgacccag gtgtcttgat ggagaaactc
     2401 gctttcaaca attaatactt tcatcattgc ttacccggtt atgaaggaat cttcgttgcg
     2461 ggcaagtatc ttccggcctt ttcgggctg
//
LOCUS       DAZFFE010000231         2469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFFE010000231 DAZFFE010000000
VERSION     DAZFFE010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.233"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(41..1447)
                     /gene="ydcR"
                     /locus_tag="ACHJ69_004854"
     CDS             complement(41..1447)
                     /gene="ydcR"
                     /locus_tag="ACHJ69_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO5017637.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKATVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDIKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKVRLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLVALTHHISIAPGNMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(1526..1762)
                     /gene="hicB"
                     /locus_tag="ACHJ69_004855"
                     /pseudo
     CDS             complement(1526..1762)
                     /gene="hicB"
                     /locus_tag="ACHJ69_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
     gene            complement(2153..>2469)
                     /locus_tag="ACHJ69_004856"
     CDS             complement(2153..>2469)
                     /locus_tag="ACHJ69_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFO5017638.1"
                     /translation="GKLHRYCLEALPKRTGTVIAGYNGTTNQDMTMSGGLIVERAPYS
                     RVRRLTPTECERLQGFPDGYTDIPWKNSSPAHRYKAIGNSMPVPVMKWIGRRIQEALN
                     EV"
BASE COUNT          647 a          600 c          554 g          668 t
ORIGIN      
        1 ttcatattac ccaacctgag aggaattaaa ttaaacgcta ttacagccgt tcttgaataa
       61 gtttgcctaa ttgttttacc gcctgttctt cacgttctcc ccactgccac gcggtattaa
      121 aacgaaaaaa acgtgaccag ttttcaccgg tagaaaacat attacccggc gcaatactga
      181 tatgatgcgt cagtgccacc aggcttaatt ctcctgcatc taacggctcg gggagctcca
      241 gccagagaaa ataaccgctg tcattgtgat gaattttgac ttccgcaggc agataacgca
      301 gcaaagcctg ccaggcgcgt tgtttacgtt ccgcaagctg gcgacgcagg cgacgaagat
      361 gagcatcgta tcggcgagta gaaaggtaat ccaccagcgc aagctgcatc ggtgagctgg
      421 tggaaagcgt actcatcaac tgcaagcgtt gaattttacg tgcatgtttt ccggcggcga
      481 cccaaccaat acgaaaacca ggcaccagac atttcgaaaa cgaagagcaa tgcaaaacac
      541 catcgtggcg atcccacgct ttcgcaggca gcggtttttc ccgtccaaaa taaagttcgc
      601 tataaacgtc atcttcaatc agcgttacgt tgtactgatt gagcaacgct accagccgca
      661 cttttttctg cggcgttaag gtaaatccaa gtgggttctg gctattagtc atcagccagc
      721 acgctttcac cggatactcc tgcaacgcca gttccagcgc ctgaagatcg atcccttctt
      781 taatatccgt cgctaccgat aacgccttca gccgtaaccg ctccagcgcc tgcaacgcgc
      841 cgtagaaaca aggattctct actatcaccc aatcgcccgg ttcagttacc gcttgcaaac
      901 tgaggtttaa tgcctctaac gccccggcgg taatgacaat ttcatcagga gaaattgtga
      961 tgccctgtaa ggcatagcga cgagcaatag cctgacgcag ttctgcgttt cctggcggta
     1021 agttttcaat cacgctcatc gccgtggcgg ttttgcttac ctgcgccagc gagcggttta
     1081 gttgttggag ggggaaaagt cgcgggtcgg gaaaggccga ggcaaacgga actaccgacg
     1141 gatcacgact ggcctgcaac atatcaaaaa tataggtgtt gatatcgacg gcttcatctc
     1201 gtgtgactgg aatgactgtc gctttcggca tttttattgc ctgtggcgca acgtaataac
     1261 ccgattgcgg tcgcgcgata atatatccct gactttcgag caactgatag gcatggctga
     1321 cagtcataaa gctcatgcca gaaagcgcca cctggtcacg caacgaaggc aaacgatcgc
     1381 cgggttgcca gatacccgac gcaatctgct cgcgtaattg ttctgcaagc tgctggtatt
     1441 ttttcattat cgaatcgtta ttatgtgcag gtgattcggc agtctatatc agttgttaaa
     1501 aatgacaact tttcattaac tgttatcaaa ccatcaccag cgataactct ttgcctagcg
     1561 cattagccgc gagctggacg gcgtcgattt ttgtcgcatg gtgcaaatta aataggcgag
     1621 taatctcctg tttatgtttg ccaattcgcc tggctaactc ttgctgagtg atttctgact
     1681 gtaaaaaagc atttaacagc aataccttag aggcgacgct caaaggcact tcaataaagt
     1741 gatcgtgact atttaatggc gaagtttacc tccgtgtgta atgtgctcga aaatagcact
     1801 aaatcgccgc aacggcagaa tgcaatgcaa aatcaggaag gaagattcca gaatttcttt
     1861 gctctaattt gcaaaactga ataataacct caagaaacga tcggcatcat agcggcaaaa
     1921 ggttagaaaa aggtggcggg atgttttcga aggcgttatc ggttgtctta ttaacgtgtg
     1981 cactgttcta tagcgcggcg atttttttga taccgatttg catttcattt gataccgatg
     2041 aaaagcgccg ataagcatct tttttgatac taaattggcg ctttcctttg cactccttaa
     2101 agacatcttt taacggcaca aaaaccaact tcataatgtc ttttgcttag gtttaaactt
     2161 cgtttaacgc ttcctgtatt cttcgaccaa tccatttcat gaccggtaca ggcattgaat
     2221 ttccaatggc cttataacga tgggctggag atgaattttt ccatggaata tctgtatatc
     2281 catcgggaaa accttgtagc ctctcgcatt cggttggcgt caacctacga acccttgaat
     2341 atggcgctct ttcaactatg agcccaccag acatagtcat gtcctggtta gttgttccgt
     2401 tatagccagc tattactgtg cctgttcgtt ttggcaatgc ttcaaggcaa tatcggtgca
     2461 attttcctt
//
LOCUS       DAZFFE010000232         2408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFFE010000232 DAZFFE010000000
VERSION     DAZFFE010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.234"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            93..1592
                     /gene="istA"
                     /locus_tag="ACHJ69_004857"
     CDS             93..1592
                     /gene="istA"
                     /locus_tag="ACHJ69_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family transposase"
                     /protein_id="HFO5017639.1"
                     /translation="MTFNTSQVSYYMTQRKKGITQHISAMKAGISVRSGRRIEKGEWA
                     KNSVRHWRTRKDPLEAVWDSMLVPLLKERPALTPTTLLEMLQDKYPGQYPNSLRRTMQ
                     RRVCEWKLQYGAEQEVMFRQRHQPGLRGLSDFTELKGVVVTIAGKLLAHKLYHFRLEW
                     SHWSWMRVVLGGESFSALAEGLQEALGQLGGVPVEHKTDSLRAAWKQQGEDGRRELTE
                     RYAALCQHYGMQGVHNNAGRGHENGSVESAHGHLKRRICQALILRGSNDFSTIEEYQA
                     FITQQVMRHNRNNQDLVKEERLHLKPLPLRRSADYDELTVRVSRSSTINVKHVVYSVP
                     SRLVGQLLRVRLWDDRLSCYVGSSEVMSCPRVRPEKGKTRARRIDFRHVIDSLAKKPG
                     AFCHATLRNDILPDDEWRRLWRRLCNHLEPDMAGRLMVHALKLAAGYDDISVVAKGME
                     QMLNTPGNVDLHRLMRFLGIKEKALPVVNVKQHNLSSYEQLLRGKGGSQ"
     gene            1589..2344
                     /gene="istB"
                     /locus_tag="ACHJ69_004858"
     CDS             1589..2344
                     /gene="istB"
                     /locus_tag="ACHJ69_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
                     /protein_id="HFO5017640.1"
                     /translation="MSNIHHLERSLRKLRLTRVGAEWHALEKRALAEGWTPSRYLLTL
                     CNEELLWRESEKLRRYKKEARLPVAKTLSEYDFSQVPELNGAQFRQLCETTDWVDAGE
                     NVLLFGASGLGKSHLAAAIVDGVVGQGYRARFYSAGELLQELRKARAQLKLNELLLKL
                     DRYRVIVVDDLGYVKRDSAETGVLFELIAHRYERGSLVITSNHPFSMWGSIFVDETMA
                     VAAADRLIHHGYMFELKGESYRKKTAKAVTSAT"
BASE COUNT          620 a          563 c          726 g          499 t
ORIGIN      
        1 atgagacgac aataactcag tccgcctggc gccgattatt ctggccggtt ttgtaacctg
       61 agtgtcattt acatgatcca acaggaaatg gcgtgacgtt caatacttct caggtcagtt
      121 actatatgac tcagcgtaag aaaggtataa ctcagcatat ctcggccatg aaggctggta
      181 tctcagtccg ttctggtcgt cggatcgaaa aaggagagtg ggcaaaaaac agtgttcggc
      241 actggcgcac acgcaaagat cctctggaag ctgtgtggga cagcatgctt gttcctctgt
      301 tgaaagagag gccggctctg acaccaacaa ctctgctgga gatgctacag gataaatatc
      361 ccggccagta ccccaacagc cttcgaagaa caatgcaacg gcgggtctgc gaatggaagc
      421 tacagtatgg tgcagagcag gaggtcatgt tccgccagcg acatcagccc ggtctgcgag
      481 gtctgtcgga ctttactgaa ctgaaaggtg tagttgtcac catcgccggt aagttgttgg
      541 cgcataagtt gtatcacttc cgtctggaat ggagccactg gagctggatg cgggttgtgc
      601 tgggtggtga gagcttctct gctctggctg aaggtctgca ggaagccctc ggacaactgg
      661 gcggagtgcc ggtagaacat aaaacggaca gcctgagggc agcatggaaa caacagggcg
      721 aagatggacg ccgcgagctg actgagcgtt atgctgctct ctgtcagcac tacggaatgc
      781 agggcgtaca caataatgcc ggtcggggcc acgaaaatgg ctcggttgaa agtgcccacg
      841 gacatctgaa aaggcgtatc tgtcaggcgc tgatactgcg gggcagtaac gacttcagca
      901 ccatagaaga atatcaggcc ttcatcactc agcaggttat gcggcacaac cgtaacaatc
      961 aggatctggt caaggaagaa cgtcttcatc tgaaaccgct gccgcttcgt cgcagtgctg
     1021 actatgatga gctgactgtg agggttagcc gcagcagtac catcaatgtg aagcacgtcg
     1081 tctacagcgt accttcccgg cttgtaggtc aactgttacg ggtccggtta tgggacgatc
     1141 gtctgagctg ttacgttggc agcagcgagg tcatgagctg cccacgtgtc agaccagaaa
     1201 aagggaagac gcgggcccgt cgtatcgact tccgacatgt gatcgacagt ctggcaaaaa
     1261 agcccggtgc gttctgccat gcaacgctga gaaatgacat cctgccagac gatgaatggc
     1321 ggaggctgtg gcgtcgctta tgtaatcatc tggaacccga catggcaggc aggctgatgg
     1381 tacatgctct gaaactggct gcaggatacg acgatatctc agtcgtggca aaaggtatgg
     1441 agcagatgct gaataccccg ggaaacgtgg atctgcaccg gctgatgcgc ttcctgggta
     1501 taaaggaaaa ggcgttgccg gtagtcaatg tgaaacagca taacctgagc agttatgagc
     1561 aactactgcg tggcaaggga ggttcgcagt gagcaatatc catcaccttg aacgcagcct
     1621 gcgtaaacta cgcctgacac gagttggagc tgaatggcac gctctggaaa aacgagcact
     1681 ggcagaaggc tggacaccat cgcgctatct tctgacgcta tgcaatgaag aactcctgtg
     1741 gcgcgagagt gaaaaactgc gtcgttataa aaaggaggcc cggttgccag ttgccaaaac
     1801 gctaagcgaa tacgacttca gtcaggtgcc ggaactgaat ggagctcagt tccggcaact
     1861 ctgtgaaacg acagactggg ttgatgcagg agaaaacgtt ctgctgttcg gagccagcgg
     1921 gttggggaaa agccatctgg cggcagcgat cgtggatggc gtagtaggcc agggctaccg
     1981 ggcccggttc tacagcgcag gagagttgtt gcaggaacta cgtaaagcca gagcgcagtt
     2041 gaaactgaat gagctgctac tgaaactgga tcgctaccgg gtgatagtgg tggatgatct
     2101 tggctatgtc aaacgcgaca gcgccgaaac gggagtactg ttcgagttaa tagcgcatcg
     2161 ctatgaacgt gggagcctgg tgataaccag taaccatccg ttcagcatgt ggggcagcat
     2221 cttcgtggat gagactatgg cggtggcggc ggcagaccgg ctgatccatc acggatatat
     2281 gttcgaactg aaaggtgaaa gctacaggaa aaagacagcg aaggcagtaa caagcgcgac
     2341 ttgatgtcgc actgaagggt gcggccagta tagttggcgc gagtcggcaa aactagttga
     2401 cgtctaat
//
LOCUS       DAZFFE010000233         2361 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFFE010000233 DAZFFE010000000
VERSION     DAZFFE010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.235"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..203
                     /locus_tag="ACHJ69_004859"
     CDS             <1..203
                     /locus_tag="ACHJ69_004859"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017641.1"
                     /translation="TACWCLMAVLPDAQNLLIVQKVQMMNKERCRTIGRKRYTTGAMC
                     FMLFVWLGGVLALVATAAVVMQ"
     gene            384..2009
                     /locus_tag="ACHJ69_004860"
     CDS             384..2009
                     /locus_tag="ACHJ69_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017642.1"
                     /translation="MPYIDITTMRGMMPRVVTSMLPEHSAVLAEDCHFRFGVITPERQ
                     ISGVEKTFTIKPKTIFHYRDDFWFAWPDVVDVIRSPIAQDPHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLAPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPAKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDANGNAALATEQIISPEQWQSQFNPASIVAYPWRGE
                     YIACYTKPDGKQDVFVFNPAGMDIRYLSTPFDCACVDLVNDVMRVVSGQNMSAIAGGS
                     LPSTIRWHSKVFSLPERTSFSCLRVKSPTPERVGITVLADDVPVIHLAPGSFSGSVVR
                     LPAATGQNWQILVSGFGQVERITLSTSMSELPV"
     gene            2006..>2361
                     /locus_tag="ACHJ69_004861"
     CDS             2006..>2361
                     /locus_tag="ACHJ69_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5017643.1"
                     /translation="MTKKPWRAGKDLSAVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGAAYALNPGIEMDSSVMVVDFPSKPQNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLV"
BASE COUNT          543 a          535 c          676 g          607 t
ORIGIN      
        1 agacggcttg ttggtgcctg atggcggtcc tgccggatgc gcagaatctt ctgatagttc
       61 agaaggtgca gatgatgaat aaagagagat gccggacaat cggaagaaaa cgttacacca
      121 caggcgcaat gtgcttcatg ctgtttgtgt ggcttggtgg tgtgctggct cttgttgcta
      181 cagctgcggt ggtaatgcag taaaaaaagg ggagcaacat gctccccgac cagaagaaag
      241 acgtttgata acaattagtg agttgttacg tcttgtccag aatatcatag caacactctg
      301 ttgcagtgat accgatcgcg attttagcga attccatcat aaatcccctg atttttaagc
      361 ctgaagcagt caaaggaatt tctatgccct atatcgatat caccacgatg cgtgggatga
      421 tgccgcgcgt tgtgacatcc atgctgcccg agcattccgc tgtactggcg gaggactgcc
      481 atttccggtt tggtgttatt acaccagaac gtcagatatc cggggttgag aaaacattca
      541 caattaagcc aaaaacaatt tttcattacc gtgacgattt ctggtttgca tggccggatg
      601 tggtggatgt gatccgcagt ccgatcgctc aggaccccca cgggcgtatt tactacactg
      661 acgggcgttt tcctaaagtg acggatgcga ccattgccac aaaaggggac gggaatcacc
      721 cgacatcatc gtatcgtctt gggatccccg cgccgacgac agctcctgtc tgtactgtcc
      781 agcagggcgg tgatgtttct gacgataacc cgaatgatga tgaaacccgg ttttatacgg
      841 aaacctttgt ctcagattat ggtgaagaag gtccgccagg tccggcgtct ctggaggtaa
      901 cactccgtac tccgggaact gcggtacaac tgacgctggc tccggtgcca ttgcagaatg
      961 ccagtattaa acgtcgtcgt atttatcgtt ctgcctcagg gggaggagaa gccgattttt
     1021 tactcgtggc tgaactggat gcatccgtgc tcagttacac ggataaaata ccggcgaaaa
     1081 atcttggacc ttcgctggcg acatgggatt acctgccgcc gccggagaat atgacaggcc
     1141 tttgcctgat ggcgaatggt attgccgccg gatttgccgg taatgaagtg atgttttcgg
     1201 aagcgtatct gccgtatgca tggccggaag tgaatcgtca cacgacggca gaagatattg
     1261 tggctatctg tccgctcgga acatcactgg tggtggcgac aaagggtgag ccttatctgt
     1321 tcagtggtgt atcgccttcc acaatttctg gctccagaat tccttcaatg caggcgtgtc
     1381 tgagcaggcg gagtatggtc gcgatggagg gctttgtgct gtatgcagga acaaatggcc
     1441 tggtgtctgt tgatgcaaac ggtaatgccg ctctggcgac ggagcagatt atttcgccgg
     1501 aacagtggca gagtcagttt aatccggcct ccattgtggc ttatccctgg cgtggtgaat
     1561 acattgcctg ttacacgaaa ccggatggta agcaggatgt gtttgtcttc aacccggcag
     1621 gtatggatat ccgctacctc agtacacctt ttgactgcgc atgtgttgac ctggttaacg
     1681 atgttatgcg ggtggtatca ggacaaaaca tgtctgcgat cgccggaggg agtttgccgt
     1741 cgacaatcag atggcattca aaggtatttt cccttccgga aagaacctct ttctcctgtc
     1801 tcagggtgaa gtctccgacg ccagagcggg tgggaattac tgtgctggca gatgatgttc
     1861 cggtgattca ccttgcacct ggaagctttt cgggaagcgt tgtgagatta cctgctgcaa
     1921 ccgggcaaaa ctggcagata ctggtttccg gttttggtca ggttgaacgt attacactca
     1981 gtacatcgat gtcggagctg ccggtatgac aaaaaaaccg tggcgcgcag gaaaggattt
     2041 atctgcggtt gtggaaaata tggagatagg gacaggacaa cgtggcgatg gtcgtcatgc
     2101 ttttgtaacc cgtgaagaac tggtcggtct gaagctggcc cgtcgccgga cttcaggtgg
     2161 tgccgcatat gcccttaatc cggggattga gatggacagc tctgtaatgg tcgttgattt
     2221 cccatcgaaa cctcagaatt ttaaggcgac agggggattt ggctcggtac tgcttgaatg
     2281 ggatatgccg aattatcgcg gtcattcact gactgaaatc tggcggggta cggaggatga
     2341 ccttgctgat gccgtgctgg t
//
LOCUS       DAZFFE010000234         2351 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZFFE010000234 DAZFFE010000000
VERSION     DAZFFE010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.236"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..689)
                     /locus_tag="ACHJ69_004862"
     CDS             complement(<1..689)
                     /locus_tag="ACHJ69_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HFO5017644.1"
                     /translation="MSVVVSGTLKSPDGQVVSDANITLTALTVSPDALSGTSASAVTT
                     STGYYGMTMEPGEYAVSVTVRGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTAVKNNQASQSKESAAASAKTASESAKAATSKAT
                     ESAQKASEAAEAATRAGESEKAAAADAKEARQHAETARVAQEAAGDVLKRAEAATVSA
                     EEARRMAENARG"
     gene            complement(686..1336)
                     /locus_tag="ACHJ69_004863"
     CDS             complement(686..1336)
                     /locus_tag="ACHJ69_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFO5017645.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLAELCDYYNDAVRAVILARP
                     DAGASLETLNCVPGARQTLPDGAIQLLDVICLSDGSAIKPQSREVLDAQYPDWHMLRG
                     KPECFISSDLAPRVFWLFPAPEEAVSVDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSTLSARKKVFNGGEM"
     gene            complement(1336..1899)
                     /locus_tag="ACHJ69_004864"
     CDS             complement(1336..1899)
                     /locus_tag="ACHJ69_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017646.1"
                     /translation="MAELTDFLPYVRRHISGPLSIMMTDALSMSAVAFCRQSLLCRRE
                     ATLSPSAGEDCVLPYDAENEECVHIIRILADGRELFAGHDVEIRPGRVMHFACSPGEV
                     SVLYAVAPKAGSRQVQDELRAWSEEVAAGALERLFMQTGVSWSDPSRAQYFSVLFSEG
                     IRRAYRDTLATSPYSSYRNPVRRQRFY"
     gene            complement(1883..2344)
                     /locus_tag="ACHJ69_004865"
     CDS             complement(1883..2344)
                     /locus_tag="ACHJ69_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017647.1"
                     /translation="MSEKIAVVYIGPKPVKKDTLTGSRTLFPRLEPVHVDSALAWQLL
                     AFPDVWVRHEELDGVLKKQQQDEQLRQAQQAQEREQVALAEAENSFVVSVGGQDVDLS
                     KLTSARLATLCEAEELNIHKDPKETADAFRVRVREAFRRRVAETEQHGGTD"
BASE COUNT          530 a          751 c          540 g          530 t
ORIGIN      
        1 cctcgtgcat tctcagccat acgtctggct tcttcagcac taaccgtagc agcctcagca
       61 cgtttaagaa cgtctccggc ggcctcctga gccactctgg cggtttcagc atgttgtctg
      121 gcctctttcg cgtctgctgc cgctgccttt tcagactcac ctgcccgggt cgccgcctct
      181 gcagcctcac tggctttttg tgcagactcc gtcgctttgc tggtcgcagc cttcgcgctt
      241 tctgatgcag tcttcgcact ggcagcagcg ctttccttcg actgtgaagc ctgattattt
      301 ttcaccgccg tgtcttcgtt cagacgacga atcgtggcaa ggtcatccgc cacattgttc
      361 tgtatctgcc ggaaatctgt cagcagttct ccgggtatgc tcacctcaac aagactgcgg
      421 cgtaacagca tattgagcgt caccgtactt tcagtcccct caatacgcac acgtccgtag
      481 acagcagtct tccctctcac cgtcactgaa accgcatact cccccggctc catcgtcatg
      541 ccgtaatagc ctgttgacgt ggtgactgcc gacgcgctgg tgccactgag tgcatccggt
      601 gaaacagtca gcgccgtcag cgtaatgttc gcatctgata caacctgacc atcaggcgat
      661 ttcagcgtcc ccgaaacaac aacactcaca tttcacctcc gttaaacacc ttcttgcggg
      721 ccgacaatgt actgtcagcc ccctgtttaa tcccgagctg ctcaacaaaa ctctgataat
      781 gttgtgctgc cagtcctgac tctgcaccac cggcagcatc cttactgaat gcgcggaaca
      841 acatccagtc aaccagcggg ttaacataag cctcttccag cggtacaggc gtgtcatcat
      901 cctgcgtcag aacatacacc gcctccggaa tgcggctgac cacagcatca acactcacag
      961 cctcctccgg tgccggaaac agccagaaca cgcgcggggc aaggtcactg ctgataaagc
     1021 attccggttt ccccctcagc atgtgccagt cgggatactg tgcatcaagc acctcacggg
     1081 attgcggttt tatagcacta ccatcgctga ggcatatcac gtcaagaagc tgtattgcgc
     1141 catcaggcaa agtctgacgt gcaccaggaa cacaattcag cgtctccaga cttgcgcctg
     1201 catccggcct tgccagaatc accgcccgca cagcatcatt gtaataatca cacagctcag
     1261 ccagaggcca gcgcaccatc atcgggtcaa ccagttgcgt gttcactcgt ccgatgattt
     1321 cagtaatcgt cgtcatcagt aaaacctttg tctgcggaca ggattgcggt atgaggagta
     1381 cggacttgtc gccagtgtgt cgcgatatgc ccgacggata ccctcagaaa acagcacaga
     1441 gaaatactgt gcgcgcgacg ggtctgacca tgaaaccccg gtctgcatga acaaccgttc
     1501 aagcgcccct gcagccactt cttcagacca tgcccgcagt tcatcctgca cctgacggct
     1561 ccctgctttc ggagcaacgg cataaagcac actcacctct ccgggcgaac aggcaaaatg
     1621 cattacccgc cccgggcgga tttccacatc atgaccggca aaaagctcac gcccgtcagc
     1681 gaggatacga ataatatgta cgcactcttc attctccgcg tcatatggca gcacacagtc
     1741 ctctccggct gacggtgaca gcgtggcttc acggcggcac aacagggact gacggcagaa
     1801 tgccactgcc gacatggata atgcatccgt catcataatg ctcagcggac cactgatatg
     1861 gcgacggaca tacggtaaaa aatcagtcag ttccgccatg ctgttcagtc tccgcaacac
     1921 gacggcgaaa tgcctcacgc acacggacac gaaatgcatc agccgtttct ttcgggtctt
     1981 tgtgaatatt cagctcttct gcctcacaca acgttgccag ccgtgcggac gtgagtttgc
     2041 tcaaatccac atcctgacca ccaacgctaa ccacaaaact gttctccgct tctgcaagag
     2101 caacctgttc tctttcctgc gcctgctgtg cctgccgcaa ctgctcatcc tgttgttgct
     2161 ttttgagcac accatcaagc tcttcatgac gaacccagac atccggaaag gccagcaact
     2221 gccaggcaag cgcgctgtca acatgcaccg gctcaagacg cgggaacagt gtacgactcc
     2281 cggtaagggt atcctttttc acgggtttgg ggccgatata gacaacggca attttttcgc
     2341 tcatataatt c
//
LOCUS       DAZFFE010000235         2333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZFFE010000235 DAZFFE010000000
VERSION     DAZFFE010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.237"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..112
                     /locus_tag="ACHJ69_004866"
     CDS             <1..112
                     /locus_tag="ACHJ69_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5017648.1"
                     /translation="AAKVLKQVMVNTGGNKRQVPAKRQNRGSRNSTDDGG"
     gene            136..432
                     /locus_tag="ACHJ69_004867"
     CDS             136..432
                     /locus_tag="ACHJ69_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017649.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRNS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
     gene            542..1363
                     /locus_tag="ACHJ69_004868"
     CDS             542..1363
                     /locus_tag="ACHJ69_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5017650.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMETMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(1660..2169)
                     /locus_tag="ACHJ69_004869"
     CDS             complement(1660..2169)
                     /locus_tag="ACHJ69_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO5017651.1"
                     /translation="MKKWMLAICLMFINGICEAADCFDLAGRDYKIDPDLLRAISWKE
                     SRYRVNAIGINPVTGYGSGLMQVDSQHFNELARYGIKPEHLTTDPCMNIYTGAYYLAI
                     AFKKWGVSWEAVGAYNAGFRKTERQNQRRLAYASEVYRIYTWIKSSKGIRVPTTKKSL
                     SQINSVQKN"
BASE COUNT          641 a          501 c          534 g          657 t
ORIGIN      
        1 tgcggcgaag gttctcaaac aggtgatggt gaataccgga gggaacaaaa ggcaggtccc
       61 cgcaaaacgt caaaaccgcg gctcccgaaa ctcaacagat gatgggggct gaaaacatca
      121 gatacaggag gaattatgag cgcattttct tttaacacca cttatcagcc aacaggcgat
      181 cagcagaaag acgcaatcgc acaaatcggg atcatgcaga atcgtgctgt tcaggcaaat
      241 cttgcatatc agagctgccg gaattctggt gcactcttta aggtgctcca ccaggtcaat
      301 aatgagctgc atgacttact ggatagcctg gagaatcata ctcctcttgt cagaaaaaac
      361 gctgatgagt tgatcgcgct gttactgctt ttcactcggc aggttggtca gtcccgaact
      421 gacctgattt gatggtcctt cccctcggaa ttgttgtctt tttgttaacg ataaccgagg
      481 gggatgatat taagcgaaaa aacttttatt aattttatgc cttcataaag gagttactgt
      541 tatgcgatta gcttcccgtt ttggtcgata taattctatt cgccgtgagc gcccgttaac
      601 ggatgacgaa ttaatgcagt ttgttccatc tgtcttttct ggtgataaac atgaatcccg
      661 gagtgagcgt tatacatata tcccaaccat caatatcatc aataagttac gtgatgaagg
      721 tttccagcca ttctttgcct gtcagagtcg ggttcgtgat ttgggacgcc gcgaatacag
      781 taaacatatg ttacgtcttc gcagggaagg gcatattaac gggaaagaag ttcctgaaat
      841 tatcctgctt aattcacatg atggttcatc cagttatcag atgatcccag gaatttttcg
      901 ttttgtctgc acaaatggcc tggtgtgtgg gaataatttt ggcgaaatcc gcgttccaca
      961 taaaggtgat attgtcgggc aggttattga gggagcgtat gaagtgctcg gtgtctttga
     1021 taaagtcact gaaaatatgg agacaatgaa agagatacat cttaacagtg acgaacaaca
     1081 tttatttggc agagctgcat tgatggccag gtacgaagat gaaaataaaa cgccagtgac
     1141 gccagaacaa ataattactc cccgccgttg ggaggataaa cagaacgatc tctggacaac
     1201 ctggcagcgg gttcaggaga atatgataaa aggtggatta tcggggcgaa gtgcctccgg
     1261 taaaaatacc agaacaagag ccattacagg cattgacggt gatatccgaa tcaacaaagc
     1321 attatggatg attgccgaac agttcaggga gtggaagtca tgataatata agtaaagccc
     1381 cgaaaaattt ttcggggctt tacttatatt atcatcctgc ggcattgctg tgtcgtctga
     1441 tatgcctcaa tttcatcaac tggcttttcg gtagtctgta aatattgtgc ccgcgaaaaa
     1501 tggtctggca ggaggctgcg cagttaccgg atatatataa gatgggatcc cgctttgcgg
     1561 ggcttcggcc agtcagcatt atcactcata tgatattttt gtgtgtggcc ttccatgccg
     1621 ctgtcgcggc ataaccggta tttgacaatg tatacaatat taatttttct gcacactgtt
     1681 aatttgggaa agtgatttct tcgtggtcgg aacccggatg cctttactgc tctttatcca
     1741 ggtataaatc cggtaaacct ctgatgcgta ggcaagacgt ctctggttct ggcgttcggt
     1801 cttcctgaat ccggcattgt atgcaccaac ggcctcccag gagacgcccc attttttaaa
     1861 ggctattgcc agataataag caccggtata aatgttcatg cagggatctg ttgtcagatg
     1921 ttccggctta attccatagc gggccagttc gttaaaatgc tgggaatcta cctgcatcag
     1981 tccgctgcca tatcccgtta ccggattaat accgatggca ttaacccggt aacgggattc
     2041 tttccatgat atcgctctca gtaaatccgg gtctattttg taatcccgac ctgcaagatc
     2101 aaagcaatcg gcggcttcgc agatcccatt tataaacatc aggcagattg ctaacatcca
     2161 ttttttcatt tttccacctc tggtgacttt atccgtaaat aatttaacct actccacaaa
     2221 aaggctcaac aggttggtgg ttctcaccac caaaagcacc acaccccacg caaaaacaat
     2281 tttttgctga tttttcttta taaatagagt gttatgaaaa attagtttct ctt
//
LOCUS       DAZFFE010000236         2325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFFE010000236 DAZFFE010000000
VERSION     DAZFFE010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.238"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..856
                     /locus_tag="ACHJ69_004870"
     CDS             <1..856
                     /locus_tag="ACHJ69_004870"
                     /inference="COORDINATES: protein motif:HMM:NF039218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HFO5017652.1"
                     /translation="VSYFYFNGLPFRVNDYFKEKISKSADFHTQQYGGAGYKDIGVYD
                     LGDKEKSKYSAILIGDSFARQYASAIDKNPYGMKLITSLKDGCYFSIRMTEFNGGARD
                     DDCPTHLMTALDISRDQNLPVILSMRWYGYKYMIGPKGGSPKTFISDKEYIDEVVSNV
                     KEIANAFNGVKIVVIGSTPGVEGVGGIESCVSRPDYLPLVCANNLVTKEKDNINKKIN
                     ESLKIELSKSKNILFIDPYDYMCSNGECATMSHDYKYLYSDNTHLSKDGAYALWSSIG
                     KNISEYIK"
     gene            complement(920..>2325)
                     /locus_tag="ACHJ69_004871"
     CDS             complement(920..>2325)
                     /locus_tag="ACHJ69_004871"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017653.1"
                     /translation="ETLRTIEPSFTNQNITVRGYYPDTPGLGGGTFIAFSSSEADDGV
                     NIFVTAGGKRWKRTGSHIDIPVENGGMMTSRTAEQNSDAFERLTACLPYEGGTLRLNG
                     FYDIKYGAIVPPRVTLEGCGMDSCGLIKTGNDIKTVPDRMWQGVTHSFSKDFIAAVDM
                     DSDTAGDLSGTQTRSTRIIGLSLKCTATGKSEYIIYSAISYNTRIEDVYGYRGMTGYY
                     TSDSWLQNVSNFMLHDVQDGYVVATAGTSFNINNLYVRDCSRHAIKMNNVTYSTLTCC
                     VPERVNGTAYVFLSCAGITLNGCAVENVTGSIFEVNQSFIVANGFRAVGVKDGGAEAI
                     IITQSNVSFNNSSLSEFEGSHTSKYYQVGGTTLNLISCYMPPASRVKWGGSFSVFNYV
                     SEGGEYSVWGTELWTATGYLVNGIAHVYGDNPPDASVTQYGNGARWELASPSAGNPYK
                     WIHVGGGVWKVAGTLAS"
BASE COUNT          717 a          493 c          413 g          702 t
ORIGIN      
        1 tgtatcttac ttttacttta atggacttcc ttttagggtt aatgattatt ttaaagagaa
       61 aatttctaaa tctgcagact tccatacaca acagtatgga ggtgcaggtt ataaagatat
      121 aggtgtctac gatcttggtg ataaggaaaa gtcgaaatac tctgccatat tgataggcga
      181 tagctttgct cgtcaatatg ctagcgcgat tgacaaaaat ccttatggca tgaaattaat
      241 aacatcactt aaagatggtt gttatttttc aataagaatg accgagttta atggcggtgc
      301 tagagatgat gattgcccaa cacatttgat gacggctttg gacatatcaa gagatcaaaa
      361 ccttccagta attttatcta tgcgctggta tggatacaaa tatatgattg gccctaaagg
      421 tggtagccca aaaacattta tttctgataa agaatatatt gatgaagttg taagtaatgt
      481 aaaggaaata gctaatgcat ttaacggagt caagattgta gtgataggtt caacgccagg
      541 agttgaaggg gttggaggta ttgaatcatg cgtttctaga cctgattacc ttcctttagt
      601 atgtgcaaat aatcttgtta ctaaggaaaa ggataacatt aataaaaaaa taaatgaatc
      661 acttaaaata gagttatcaa aatcaaaaaa catattattc attgatccat atgactacat
      721 gtgcagtaac ggagaatgtg caaccatgtc tcacgattat aaatatcttt attcagacaa
      781 cacccactta tctaaagatg gggcttatgc gctatggtca tcaattggta aaaatataag
      841 tgagtatata aaataaaaac cactttattg actgcacccc aaaagttgga cacccaactg
      901 agttgggtgc agttttttat tatgaagcca aagttccggc aaccttccac actccaccac
      961 ctacatgtat ccatttatat ggatttcctg cagatggcga agccagctcc caccttgctc
     1021 cattgccata ttgagtgaca ctcgcatctg gagggttatc accatagaca tgagcaatcc
     1081 catttactag atagccagtc gcggtccata gctcagttcc ccacacggag tattctccac
     1141 cctcgcttac ataattaaat acagaaaaac tcccccccca tttaactctt gatgctggag
     1201 gcatatagca tgaaattaaa ttaagagttg taccaccaac ttggtagtat ttactagtgt
     1261 gtgaaccttc aaattcagaa agtgaagaat tgttaaatga tacatttgac tgcgttatta
     1321 ttatagcctc agcgccacca tcttttacac caacagccct gaatccatta gctactataa
     1381 aagattgatt gacctcaaat atactaccag ttacattctc taccgcacac ccatttaatg
     1441 taatcccagc acatgacagg aaaacatatg cagttccatt aaccctctca ggaacgcagc
     1501 atgttaatgt gctatatgtt acgttattca tttttatggc gtggcgagag cagtctctaa
     1561 catagagatt atttatatta aatgatgttc ctgcggttgc aacaacatat ccatcctgaa
     1621 catcatgcaa cataaaatta gatacattct gcaaccaact atcactggta taataccctg
     1681 tcattcctct ataaccatat acatcttcaa ttcgtgtatt gtaactaata gcgctatata
     1741 taatatattc agactttcct gttgcagtgc attttagact caacccaatt attcttgtgc
     1801 ttcttgtttg tgttccgctt aaatcaccag cagtgtcact atccatgtct accgcagcga
     1861 taaagtcttt ggaaaaactg tgcgtcacac cctgccacat gcgatccgga actgttttaa
     1921 tatcgttacc tgttttgatt aatccacagg aatccatacc acagccctcc agcgtgacac
     1981 gtggaggcac gatagcgcca tacttaatat cgtaaaatcc attgagcctg agcgtacctc
     2041 cttcatatgg caggcatgca gtcagccgtt caaaagcatc actgttttgc tcagccgttc
     2101 tggaggtcat cattccaccg ttctctaccg ggatatcaat atggcttccc gttcgcttcc
     2161 agcgttttcc tcctgccgtt acaaagatat ttactccatc gtcagcctct gaactggaaa
     2221 aggctataaa cgttcctcca ccaagacctg gtgtatcagg gtaatatccg cgaactgtta
     2281 tattttgatt tgtgaatgaa ggctcaatag ttcttagtgt ctcaa
//
LOCUS       DAZFFE010000237         2221 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFFE010000237 DAZFFE010000000
VERSION     DAZFFE010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.239"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..482)
                     /locus_tag="ACHJ69_004872"
     CDS             complement(<1..482)
                     /locus_tag="ACHJ69_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO5017654.1"
                     /translation="MNTSEKQLNNFAVVCGVALRGLLRPLPVTTVEWADQNYYLPPES
                     SYLAGRWKTLPFQVAIMNCMGNDRIRTINLIKSARVGYTKMLMGVIGYFIEHKNRNSL
                     LFQPTDSAAEDFMKSHVESTLRDVPCLKILSPWLGRKHRDNTLTLKRFTSRVGFWCLG
                     G"
     gene            complement(454..1002)
                     /locus_tag="ACHJ69_004873"
     CDS             complement(454..1002)
                     /locus_tag="ACHJ69_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5017655.1"
                     /translation="MNVNKKKLAEIFNVDIRTIDRWQTQGLPQISGGGKGVEVIFDSA
                     KAIEWYAQREADIENEKLRKEVEDLRAASESDLQPGTIDYERYRLTKAQADAQELKNA
                     RDSGQVIDTGFCLFALGRLAQEISAILDSIPLSMQRQFPALTPAMLDFLKTDIAKAAN
                     RCASTAEKLPEMLDEYLREAAK"
     gene            complement(1450..1674)
                     /locus_tag="ACHJ69_004874"
     CDS             complement(1450..1674)
                     /locus_tag="ACHJ69_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017656.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            complement(1760..1945)
                     /locus_tag="ACHJ69_004875"
     CDS             complement(1760..1945)
                     /locus_tag="ACHJ69_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO5017657.1"
                     /translation="MRELKMKLFALTLPLVVSACVSTPPVQVPCVKPPAPPAWIMQPA
                     PDWQTPLHGIISPSESG"
BASE COUNT          579 a          584 c          504 g          554 t
ORIGIN      
        1 ccacccaggc accagaaccc cacacgggac gtgaatcttt ttaatgtcag tgtgttatcc
       61 cggtgcttac gccccagcca gggggaaaga attttcagac agggaacatc ccgtaatgtt
      121 gactccacat gagacttcat aaaatcttca gcggcagaat ccgtaggctg aaacagaaga
      181 ctgttacggt ttttatgctc aataaagtaa ccaataacgc ccatcagcat tttggtgtaa
      241 ccgactcgag ctgattttat aagattaatt gtgcggatcc ggtcattacc catgcagttc
      301 attattgcca cctgaaaggg caacgtcttc caccgcccag caagataaga ggactccggg
      361 ggcaaataat aattttgatc ggcccattca acggtggtta caggcagtgg gcgtaaaagc
      421 ccacgaagtg ccactccaca aacaacagca aagttattta gctgcttctc tgaggtattc
      481 atccagcatc tccggtaact tctctgccgt tgatgcgcat cggtttgccg cctttgctat
      541 atcggttttc aggaaatcaa gcatagcggg cgtcagtgcc ggaaactgtc tctgcatgga
      601 caatggaatg gagtcaagaa tggcagagat ctcctgtgcc agtcttccca gagcaaaaag
      661 gcaaaaccct gtatcaatga cctgcccact gtcgcgggca tttttcagtt cctgtgcatc
      721 tgcctgtgcc ttcgtcagtc ggtaacgttc gtaatcaatg gtgccgggct gaaggtctga
      781 ttcgctggca gccctcaaat cctcgacctc tttacggagt ttttcatttt caatatcagc
      841 ttcgcgctga gcataccatt cgatcgcctt cgcagaatca aagatgacct caacaccttt
      901 tcctccacca gatatctgag ggagcccctg agtttgccag cgatcaatag tgcgtatatc
      961 gacattaaag atttcagcaa gcttcttttt attaacattc attcagcaat tccttatcaa
     1021 aaatcagtta cgacatgaaa tgccaaaaaa gacagatttt tcagcgttct gatgtcgttt
     1081 ctttcttgat gattatttct ttaaaaacaa taagttaaca acaagaagaa ctgacatgct
     1141 ttttcccgaa aaattttcat aaatagcgaa aacccgcgag gtcgccgccc cgtaacggcc
     1201 cggatcgccg gaaaggaccc ggcaaaatga taatgattgt cagttgcaac aaaatccagt
     1261 ttcttccacc atcgcaccgg accagcgacc atgaggggac aacgccgcgc tccgttaacg
     1321 cggtaaaccc cggtgtgtat cgtttttgat tatccccgca cactctcgca gaggagtctc
     1381 cctgtcgggc tgcggtctct gttaatgcag gaatacggcg acaataccgc gcatggataa
     1441 taaggtcgct caacacactg gctgtaatgc agcggatacc atgcggcatt tagcggcatt
     1501 catcgtacac tcaacggtta gctcttcatt cgtggcattc acctgaaagg tccgggagtg
     1561 taattgcgta catttaccac tgaacgaacc ttcaacaaga acacgaccac gctgcaaaat
     1621 acggaacgga attgttccct gaaaaggctt tacggttacc agtaatttct tcatacatcc
     1681 tccggataat aaaaagacta cttaatgcac tgagtacgga tatattcctg cgccccttcc
     1741 agttgcttct gcatcgtcat cagccgctct ctgagggtga aataatcccg tgtaacggtg
     1801 tctgccagtc gggggccggt tgcattatcc acgcgggcgg tgccggtggc ttcacgcacg
     1861 ggacctggac aggtggcgtt gatacgcagg cgcttacgac cagcggcaac gtcagcgcga
     1921 aaagtttcat tttcagctct cgcatcagct aattccctcg agtattttgc atcgagcgca
     1981 gcaacatcgc gctggcgcac ctgcatatca gtaatggttg cgttcgccag cttcagttca
     2041 ctggctttgt tatcgcgctg cgctttgtag gtaatcgcgt tatcacggta atggtctgtt
     2101 gccatccaca gcgctccaca ggccaccagc agaataacga taaacgcgga aagcattcgg
     2161 tttatgttca ccccagcagc cccgacgaag acaacatcat ccaggccatg gaaagaaaaa
     2221 g
//
LOCUS       DAZFFE010000238         2103 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFFE010000238 DAZFFE010000000
VERSION     DAZFFE010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.240"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1388)
                     /gene="tssI"
                     /locus_tag="ACHJ69_004876"
     CDS             complement(<1..1388)
                     /gene="tssI"
                     /locus_tag="ACHJ69_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFO5017658.1"
                     /translation="MSLKGLRFTLEVDGQEPDTFAVVSFRLIQNQSYPFVMSVDVASD
                     SFMQTAEMLLEKNATLTIWQGVIPLRYVTGVVAGFGMQENNGWQMRYHLRIEPPLWRC
                     GLRQNFRIFQQQDIRTISATLLNENGVTEWTPLFYEDHPAREFCVQYGESDLAFLARL
                     WAEEGIFFFERFAADSPEQKLTLCDDVAGLSQAGEFPFNPDTSAGAETECVSMFRYEA
                     HVRPSSVQSQDYTFKVPDWPGMYEQQGESLNGQLEQYEIFDYPGRYKDEQHGKDFTLY
                     RIESLRSDAEKATGQSNSPKLWPGTRFTLTGHPQKMLNREWQVIQSILSGDQPQALHG
                     SQGRGTTLGNQLEVIPADRTWRPRVQSKPKVDGPQSAIVTGPAGEEIFCDEHGRVRVK
                     FHWDRYHGMTEESSCWVRVSQAWAGPGFGNLAIPRVGQEVIVDFLNGDPDQPLVMGRT
                     YHEDNRSPGDLPG"
     gene            complement(1407..1691)
                     /locus_tag="ACHJ69_004877"
     CDS             complement(1407..1691)
                     /locus_tag="ACHJ69_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system PAAR protein"
                     /protein_id="HFO5017659.1"
                     /translation="MPLAAKLTDKGTQHDGYYETVITAGSSTVFIDGLPAARQGDPLT
                     PHAKPKHPPHPRKIARGSSTVFIDGLPAARTGDAIDCGGVVIGGGTVNIG"
BASE COUNT          480 a          656 c          476 g          491 t
ORIGIN      
        1 cccggcaggt cccccggcga acggttgtcc tcatggtacg tccggcccat gaccagcggc
       61 tggtccgggt ccccgttcag aaaatcaaca atcacctcct ggcccacccg cggtatcgcc
      121 aggttaccga accccggccc cgcccacgcc tgcgacacac gcacccagca cgaactctcc
      181 tccgtcatcc cgtgataacg gtcccagtga aacttcaccc gtacccgacc atgttcgtcg
      241 cagaagattt cctctcctgc gggaccggtg acaatggcgc tctgtggccc gtccaccttc
      301 ggtttgctct gcacccgtgg acgccaggtc cggtccgccg gtatcacctc taactgattt
      361 cccagcgtgg ttcctctccc ctggctgcca tgaagcgcct gcggctggtc ccctgacaga
      421 atactctgta tcacctgcca ttcacggttc agcatcttct gcggatgccc cgtcagcgta
      481 aaccgcgttc ccggccacag cttcggcgaa ttactctgcc ctgtggcctt ttccgcatca
      541 ctgcgcaggc tctctatccg gtacagcgtg aaatccttgc cgtgctgttc atccttgtac
      601 cttcccggat aatcaaatat ctcatactgt tcaagctgtc cgttcaggct ctcaccctgc
      661 tgctcataca tccccggcca gtccggcacc ttaaacgtgt aatcctggct ctgcaccgat
      721 gacgggcgga catgcgcctc ataacggaac atactgacac actccgtctc cgctcctgcc
      781 gatgtgtccg ggttaaacgg aaactccccc gcctgtgaca gccccgccac atcgtcgcac
      841 agcgtcagct tctgctccgg actgtccgcc gcaaaccgct caaagaagaa aatcccctcc
      901 tccgcccaca gccgcgccag aaacgccagg tccgactccc cgtactgcac acagaactcc
      961 cgcgccggat ggtcctcata aaacagcggc gtccactccg tgacgccgtt ctcgttcagt
     1021 aacgtggccg atatcgtccg gatatcctgc tgctggaaga tgcggaagtt ctgccgcagc
     1081 ccgcaccgcc ataacggcgg ctcaatacgc aggtgataac gcatctgcca gccgttgttc
     1141 tcctgcatac caaaccctgc caccacgcct gtgacataac gcagcgggat gacaccctgc
     1201 cagatagtca gcgttgcgtt cttctccagc agcatctccg ccgtctgcat aaaagaatcg
     1261 ctggcgacat ccacactcat cacaaacgga taagactggt tctggattaa ccggaagctc
     1321 accactgcaa aggtgtccgg ctcctggccg tcaacctcca gcgtaaagcg aagacctttt
     1381 aaggacataa atacctctca gacatatcag cctatattta ccgtgccgcc accaataacc
     1441 acgccgccgc agtcaatagc atcaccggtt ctggcggcgg gcaggccgtc aataaagacg
     1501 gttgacgagc ctctggcgat ttttcgggga tgaggtggat gcttcggttt agcatgcggc
     1561 gtcagcggat ctccctggcg ggctgcgggg agaccatcga taaaaacggt ggatgacccg
     1621 gcggtgatga cggtctcgta ataaccgtcg tgctgggtgc ctttgtcggt aagtttggct
     1681 gctaaaggca taattcctcc tgttcagaat ttctacagca gcaggtttca aaaatccgga
     1741 gaaatattca acgaaatatt aatcacttca ccggatattc tgatgcgtac cttcactggt
     1801 ctatttaagt aatacaaatt gttggcgtgt attaagtttc attttgttct ccttaattat
     1861 gtaaggagta tttgatcatg tataagcaat gaaaaacagc ttcaggaaat aaggagtatc
     1921 tcaattttta aacataaaat gaaaatcatt cagtacaaaa aagcagcatt acgtaatctt
     1981 tccctgatta aatacgctac cgcccagccc tgataacatg atctgcactg cgctatcata
     2041 tcgacatgtt tctctacatg cgaaaagatg ttttattttc agattatatt aaattgatat
     2101 tta
//
LOCUS       DAZFFE010000239         2101 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFFE010000239 DAZFFE010000000
VERSION     DAZFFE010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.241"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(60..1025)
                     /locus_tag="ACHJ69_004878"
     CDS             complement(60..1025)
                     /locus_tag="ACHJ69_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFO5017660.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRTPEKDKSD"
     gene            complement(1891..>2101)
                     /locus_tag="ACHJ69_004879"
     CDS             complement(1891..>2101)
                     /locus_tag="ACHJ69_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017661.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          580 a          550 c          440 g          531 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggtgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttacc cccccaaaac aagcaccccc gaacggctta ccccaacctt
     1381 ttccacacgc ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgatatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatac tgaggataat
     1801 gctgaggata atgcaggata gacagagcac tagagattgc atgcaatctc gtacctatca
     1861 cctcagcctg ccgctggctc ttttccagaa ctaagaatgc gttcttaaaa atccaaactt
     1921 gataccgttt aaatctattt tcacaaaaag agtttttacc ctctcgtttg ttttctgaat
     1981 atataaaatt aattcatcct caaaaccttc gtgcggatga aaaccaaatg tgccaaaact
     2041 taatccatca ataagcaaaa taaggttttt aaacctgtca ggaatcaccg cttcacttct
     2101 t
//
LOCUS       DAZFFE010000240         2081 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFFE010000240 DAZFFE010000000
VERSION     DAZFFE010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.242"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>2081
                     /locus_tag="ACHJ69_004880"
     CDS             <1..>2081
                     /locus_tag="ACHJ69_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD5 domain-containing protein"
                     /protein_id="HFO5017662.1"
                     /translation="TGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERITD
                     ADPMEQPAFMRDPRFRGFTGDDTEVQARLARGNAPTAEELVRSQMAEGDAGPTAQELT
                     ERPRLPAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGRAQAPE
                     NAAGRQGEILEGEPVRRGLPSPDEQNATAPVREGLPAPEQRGVDMPHPESLPRMVRDS
                     LPELAQQAEARRQTGDNRQTITDVPDTEVPVPVDKPSTHQQARGAKIEDFGEEIKGAA
                     KHRYAQLAETLGKTLEDRDYFTQPLSKVFPKPDYAKLANEGADADTLAMIALYRSDIP
                     AKTKLNAVKWVESVKSIRTSVAGMLEGKVTAGRLAEWMEGRMPSRYADTWQLLRTLPP
                     SQMDKASAYRVVSGVYQAAGGKRYDPSQKLYSLRNKDNKGSNLFFSESRDELLTKAKA
                     WFAEQEEKSRAKGDEKTAPSPDDKISFDVYRNTRSGDIFIAYGKNKMRVRGGFKSASD
                     ARKYIDSHRDELVRHVKEMREISREEQRNATNRDRTGPERRKGDVSPEQFSDAFGFRG
                     VQFGNYVEGPRRQADLNRAYDSLHDLADVLNVPTKALSLNGRLGLAFGARGKGKAAHY
                     EPGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSTDGKITSGGDYMTEAQRAGRVF
                     KDGRYVDAEYPV"
BASE COUNT          522 a          490 c          644 g          425 t
ORIGIN      
        1 accggcctga aaccgtccgt agtgaagaaa ggcgagaatc agtatgccgt tgaaatggat
       61 aatcctgcgt tctctgaaga tgtggcaacg gagaccatta acactctggc tgacggagag
      121 cgtattactg atgctgatcc gatggaacag cccgcgttca tgcgtgaccc gcgattccgt
      181 ggttttacgg gggatgatac agaagtgcag gcccgtcttg cccgtggcaa cgcgccgacg
      241 gcggaagaac tggtgcgttc ccagatggct gaaggtgatg ccggtccgac agcacaggag
      301 ttaactgagc gtccacgcct tcccgctccc ggcgatattc atcccggaca gggatatccg
      361 ttaccgggag aagtggcgcg tacgccggat gaaaatcagg ccggacgtgg tggtcgtttt
      421 accacaaccg gtgaggtgaa gggccagagt ttccagaaag gacgtgcgca ggcaccggaa
      481 aatgccgctg gtcgccaggg ggaaatactg gagggcgaac cggttcgtcg tggtctgccg
      541 tcaccggatg agcagaacgc cacagcacca gtgcgtgaag gacttcctgc acctgaacag
      601 cgtggggttg atatgccaca tcctgaatca ctccctcgta tggttcgtga ctcccttccg
      661 gaactggcac agcaggcaga agcacgcaga cagaccgggg ataatcgcca gaccataacc
      721 gatgttccgg atactgaggt gccggtgccg gtagataaac cgagcactca ccagcaggcg
      781 cgtggtgcaa aaattgaaga ctttggcgag gagattaagg gcgcggcaaa acaccgttat
      841 gcacagcttg ccgaaacact gggtaaaacg ctggaagaca gggattattt cacacagccg
      901 ctgagcaaag tgttcccgaa accggactat gcaaaactgg cgaacgaagg ggccgacgct
      961 gacacactgg caatgattgc gctgtaccgc agtgatattc ccgcgaaaac gaaactcaat
     1021 gcggtgaagt gggtggagag tgtaaagagc atccgcacaa gcgtggcggg aatgctggaa
     1081 ggaaaagtga ctgccggacg gcttgctgag tggatggagg gaagaatgcc ttcccgttat
     1141 gcggatacct ggcaactgtt acgcacgctg ccaccttcac agatggacaa ggcttctgct
     1201 tatcgggtgg tatccggtgt gtatcaggcg gccggaggga agcgttacga tccgtcacag
     1261 aaactttatt cactgcgcaa taaggacaat aaggggagta acctcttttt ctcggaaagc
     1321 agggatgaat tactgacaaa ggcgaaagcc tggtttgcag agcaggagga aaagtcacgg
     1381 gcgaaaggtg atgaaaaaac agcaccgtca ccagatgaca aaatcagctt tgacgtttac
     1441 cggaataccc gtagtggcga tatttttatc gcttacggca aaaacaaaat gcgggtgaga
     1501 ggtggtttta agtcagccag tgatgcgcgt aagtacattg attcacatcg tgatgagctt
     1561 gttcgtcatg tgaaggagat gcgggagatt tcgcgtgagg agcagcgtaa cgccactaac
     1621 cgcgaccgca ccggaccaga acgccgcaaa ggggatgttt cgcccgagca gttcagtgat
     1681 gcgtttggtt tccgtggtgt gcagtttggt aattatgtgg aaggtccgcg tcgtcaggct
     1741 gatttgaacc gggcttatga ctcgctgcat gacctggctg acgtgctgaa cgttccgaca
     1801 aaagcgcttt ccctgaatgg tcgtcttggg cttgcttttg gtgcccgtgg taagggtaag
     1861 gcggcacact atgagccggg tgaggtggca atcaacctga caaaaggtaa cggaccgggg
     1921 gcgctggccc acgaatggtt ccattcactg gataattatt ttggtcgtta tgatgtttcc
     1981 actgacggga agatcacgtc aggtggcgac tatatgacgg aagcacaacg tgccgggcgc
     2041 gtatttaaag acggcagata tgttgatgcg gaatatccgg t
//
LOCUS       DAZFFE010000241         2070 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFFE010000241 DAZFFE010000000
VERSION     DAZFFE010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.243"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..789)
                     /locus_tag="ACHJ69_004881"
     CDS             complement(<1..789)
                     /locus_tag="ACHJ69_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO5017663.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTI
                     AQAKELFALAKSKGLIVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMP"
     gene            1123..1611
                     /gene="yhhY"
                     /locus_tag="ACHJ69_004882"
     CDS             1123..1611
                     /gene="yhhY"
                     /locus_tag="ACHJ69_004882"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO5017664.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSEQMWLER
                     LTARPGIKQLVACIDGIVVGHLTIDVQQRPRRSHVADFGICVDSRWKNHGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            1847..>2070
                     /gene="tssD"
                     /locus_tag="ACHJ69_004883"
     CDS             1847..>2070
                     /gene="tssD"
                     /locus_tag="ACHJ69_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO5017665.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTTESIGNRWQSGHEDEIFTFSL
                     LNSITNTGLGSQFHGITFCKLIDKSTPLFI"
BASE COUNT          569 a          458 c          493 g          550 t
ORIGIN      
        1 cggcataata ttagccttca ggctggtttc ctgctggtcg ataccgtatt taataaacga
       61 acctttctta ccgtgaacga taaatttcgg ataatcgatt ttcaccagat ggctggtttt
      121 gacgatggct tttagatcgc cataaaacag ctgcgcttca aaggtgtcgt ccggattggc
      181 tttattacgc aggctgcgga tgtcataagc gacgtgatcc gggcgaccga acagagaaat
      241 aatctggtcc atcgtatgca caccaaggcc atagaacgcg ccatcctgcg gcagccccgg
      301 tttggtttct gccaccgggc ggtaatagtc aaaatggctt tccacttcga caatctctcc
      361 cagcttgccg ctttcaatcg cttttttcgc tgtcaggaag caggaatcaa aacgacgatt
      421 ctgatacggc gtaacgatca gccctttgct tttcgccagt gcaaacagct ctttcgcctg
      481 cgcaattgtc ggagtgaacg gtttttcgac cagcacattt ttcccggctt ccagcgcgcg
      541 cttcgcgtac tcgaagtggc tgtccgcgtg ggtgcagacg acaaccagct taacatcggg
      601 atcgtttagc acttcgtcga gatcgctggt gaaatggata tgggaataaa tgggggcctg
      661 ttcttccggc ttcgcatggc ggcgaaaaat atgcgcgaca tgccagctat ccttgcggtt
      721 aagtacatac ggcagatggt aacgggtggt gcttttgccg aagccaataa aggcgcagtt
      781 aatgaccatg atttcgtcct ttttaaggtg gttatttaca ccttagcgca aagcggacgt
      841 ggttcctact ggagtgcgca taacgaacac aagcacttcc cgaggataaa ttgagaacga
      901 aaggtcaaaa aaaaagccag cacccggctg gctaagtaat actggaagca atgtgagcaa
      961 tgtcgtgctt tcaggttctc cgcgagggtc ttcctgatcg cgagacaata ataatcattc
     1021 tcattcgcac ttgtccaaca ctttttgcaa aaaaatgcat ttgactcgca tttgaaagtc
     1081 aatgatgttg aaagggacat ttaccccaaa gaggacaaag gaatgagtga gatagtaata
     1141 cgccacgcag aaacacgcga ttacgaggcc atcaggcaga ttcacgccca gccggaggtg
     1201 tattacaaca cgctacaggt gcctcatcct tcagaacaaa tgtggctgga acgactcacc
     1261 gctcgccctg gcatcaagca gttagtcgcc tgtattgatg gaatcgtcgt aggccatctc
     1321 accattgacg tgcaacaacg cccacgccgc agtcatgttg ccgattttgg tatctgtgtc
     1381 gactcccgtt ggaagaacca cggcgtcgcc agcgccctga tgcgagagat gattgaaatg
     1441 tgcgacaact ggttgcgggt agatcgtatt gagctaacgg tgttcgtcga taacgcgcca
     1501 gcaattaagg tctataaaaa atttggcttt gagattgaag ggactggcaa gaagtacgcg
     1561 ctgcgtaatg gtgaatatgt cgatgcgtat tatatggcgc gggtgaagta agatagtgcc
     1621 ctttttctga gatggaaaaa gggtgtcatt caaaatcggc ataccttcct ttaatgtatt
     1681 tattttccca ataaacatat gagatttacc tcataattac ttcctaaagt gtaatatttt
     1741 attttttaat atatacgcct acaatttcct ggagtaaata aataacaatt aacaagcata
     1801 atattgccat tgataaaata gcatgccata aaaggacttt tcagggatga gtaatattgt
     1861 ttatctgaca gtaacgggag aacaacaagg aagcatctcc gcaggttgtg ggactactga
     1921 atctataggt aatcgctggc agagcgggca tgaggatgaa atatttacat tctcactctt
     1981 aaatagtatt actaatacgg ggcttggttc acagttccat ggtataacat tttgtaaatt
     2041 aattgataaa agcactccat tatttattaa
//
LOCUS       DAZFFE010000242         2019 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFFE010000242 DAZFFE010000000
VERSION     DAZFFE010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.244"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(56..592)
                     /locus_tag="ACHJ69_004884"
     CDS             complement(56..592)
                     /locus_tag="ACHJ69_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HFO5017666.1"
                     /translation="MLHTIHFLCPVNTATVGQLQNHCLTALSQGATELNIHISSQGGE
                     TAAGFTAYNFLKSLPVTVRTHNISNVESIANIVFLAGSERFANPLSRFLLHPLLWCFA
                     SPAADHARLREYGKCLDNDLDRFVETFNIDIGTHIRWASLIADSTILDANKALEHGII
                     NSIKTARLASNQANWWVV"
     gene            complement(592..840)
                     /locus_tag="ACHJ69_004885"
     CDS             complement(592..840)
                     /locus_tag="ACHJ69_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO5017667.1"
                     /translation="MAKGVSISPTTVRIPESLREALAVRASKNGRSVNSEIVMILQAA
                     IDEDRSPKSVESFAQQEADKFKEALLETLKTMYGKDEK"
     gene            955..1107
                     /locus_tag="ACHJ69_004886"
     CDS             955..1107
                     /locus_tag="ACHJ69_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO5017668.1"
                     /translation="MEKEISKILVRMPQSLKDAIGSRAKEECRSFNSEVIKRLIDSLK
                     REGVMV"
     gene            1370..>2019
                     /locus_tag="ACHJ69_004887"
     CDS             1370..>2019
                     /locus_tag="ACHJ69_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO5017669.1"
                     /translation="MNSIAILEAVNTSYVPFNGQQIITAMAAGVAYVAMKPIVENLGM
                     SWGTQQQKLMKQLDKFNCIHMNMVAADGKLRKLLCLPLKKLNGWLFSINPEKVRADIR
                     DKLIQYQEECFTVLHDYWTKGKAENARKKTSVDDRTPLRDAVNMLVSKKHLMYPEAYA
                     MIHQRFNVESIEELDASQIPQAVEYIHRVVLEGEFIGKQEKKTNELSAKEANSLVWL"
BASE COUNT          610 a          385 c          486 g          538 t
ORIGIN      
        1 ggattatgcc caccgtcagg tgggtttttt ataaggagta atcatgattt acccatcaaa
       61 caacccacca gtttgcctga ttggatgcca gccttgcagt ttttatggaa ttaattatgc
      121 catgctcaag agccttgtta gcatccaaaa tggtcgagtc tgctatcagg gatgcccacc
      181 taatatgggt tccgatgtcg atattgaacg tctcaacgaa gcgatcaaga tcgttatcga
      241 ggcatttccc gtactctctc aatctggcat ggtcggcggc tggggaggca aagcaccata
      301 atagaggatg taacaggaat cttgataatg ggtttgcgaa acgttctgag ccagccagga
      361 aaacgatatt agctatggat tcaacattgc ttatgttgtg agttctaacg gtaacaggga
      421 gtgacttaag aaagttatac gcagtaaagc cagcggcagt ttcccctccc tgacttgata
      481 tatggatatt taattcagtt gcgccttgag ataatgcggt gagacagtgg ttctgaagtt
      541 gcccaacagt ggcagtgtta acggggcata agaaatgaat tgtgtgcagc attatttttc
      601 atccttacca tacatggtct ttagcgtctc aagcagcgcc tctttgaatt tgtcagcttc
      661 ttgctgagca aatgactcaa ctgactttgg cgacctatct tcatcaatcg cggcttgcaa
      721 aatcatgacg atctcggagt taacagagcg accatttttt gatgctctga cagcaagagc
      781 ctcgcgtaaa gattcaggga ttcttaccgt agttggagaa attgacacac cttttgccat
      841 atcacacctt tggtattcaa tttgatagca aagtgtatgc aaaaaaattt tgactagata
      901 tactcacttt gctatctttt gtattctaaa agagttgttt gtatggaggg taacatggag
      961 aaagaaataa gtaagatttt ggtaaggatg ccgcagtcgt taaaggatgc tataggtagc
     1021 agggcaaagg aagagtgcag gtcgtttaac tcagaggtta tcaagcgcct gatagacagc
     1081 ttgaagagag agggggtaat ggtatgagta aagaatgttg tttctgcggc attagcgaat
     1141 cagacgctga tcaaacatac atttactcta aagaaacagg tcggatgctg tgtagtgact
     1201 gcgtgttgga catcataaga tacaagcatc ttggatgttc tgtcagcatt agcaatatag
     1261 gtgaagtata tgaagggaaa gatataactg atagagcaga aagttgaagc cccaactgcg
     1321 ggaacagtca gggcttcggt tgtcagtaaa tccgtggaga aaaaccaaca tgaatagtat
     1381 agcaatttta gaagcagtga acacctctta cgtaccattc aacggtcagc aaattatcac
     1441 cgccatggct gccggagttg catatgttgc gatgaagcca atcgttgaaa acctcggaat
     1501 gagctggggt actcagcaac aaaaacttat gaaacaacta gataagttca actgtattca
     1561 tatgaatatg gttgctgctg atggaaagct tcgtaagcta ctctgccttc ctttgaagaa
     1621 gttaaatgga tggctgttca gcatcaaccc cgagaaagtt cgtgctgaca tccgcgataa
     1681 actgattcag taccaggaag aatgctttac tgtgctgcat gactactgga caaagggaaa
     1741 ggcagaaaat gcacgtaaga aaacatctgt tgatgacagg actccgcttc gtgatgctgt
     1801 aaatatgctg gtcagcaaaa agcatctaat gtacccagaa gcttatgcaa tgattcatca
     1861 gcgtttcaat gtggaaagta ttgaagagct tgatgcatct cagataccgc aagccgtaga
     1921 gtacatccac agggtagtgc ttgaaggtga gttcattggc aaacaagaga agaaaaccaa
     1981 cgagctttcc gcaaaagaag caaacagcct tgtatggct
//
LOCUS       DAZFFE010000243         2007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZFFE010000243 DAZFFE010000000
VERSION     DAZFFE010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.245"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..236)
                     /locus_tag="ACHJ69_004888"
     CDS             complement(<1..236)
                     /locus_tag="ACHJ69_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFO5017670.1"
                     /translation="MFKEKLQDYTEDEFLNFLGGLRSSMKDGKSLKGKELEMYWDSLV
                     DHFIEITQHPSGSDLIFYPKSQGDDKPENILKIV"
     gene            complement(239..>2007)
                     /gene="tssD"
                     /locus_tag="ACHJ69_004889"
     CDS             complement(239..>2007)
                     /gene="tssD"
                     /locus_tag="ACHJ69_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO5017671.1"
                     /translation="YLRIIGEKQGDISSGCGTYASVGNRWQVGHEDEIFAFALTNAIT
                     STGKGVNLQGLQFCKLIDKSSPLLSNAINQNERLFIEIDLYRINKSGRWERYYYIQLR
                     NASLTAIHVNISDNNLPTECVNVNYDYILCKHLIANTEFDWLAFPAGYNSLFIPPKNP
                     PASNLNPEPLPVVNLPLSPPAVKPVYAKSCLKEKGCTDAGTAEEPAENFGQVAIFALP
                     VVDDCCGYHHPEANDVGQPAEAQTMLLFPGSVLAAQIWGKWSLSGILSATCGSYIGAL
                     ASALYIPSAGEGSARVPGRDEFWYEEELRQKALAGSTATTRVRFFWGTDIHGKPQVYG
                     VHTGEGTPYENVRVANMQWNEQTQRYEFTPAHDVDGPLITWTPENPEHGYVPGHTGND
                     RPPLEQPTILVTPIPDGTDTYTTPPFPVPDPKEFNDYILVFPAGSGIKPIYVYLKEDP
                     RKLPGVVTGRGVLLSPGTRWLDMSVSNNGNGAPIPAHIADKLRGREFKTFDEFREALW
                     LEVSQDPELIAQFSSGNQTRIKQGLTAKAPIDGWYYGPKEIVKKFQIHHRVAVEYGGS
                     VYDIDNLRIVTPRLHDEIHYRR"
BASE COUNT          516 a          495 c          448 g          548 t
ORIGIN      
        1 ttgacaattt tcaggatgtt ttcaggctta tcatctcctt gacttttagg gtagaaaatt
       61 agatcgctcc ctgacggatg ctgtgtaatt tcaataaaat gatctactaa tgaatcccag
      121 tacatttcta gctccttacc ttttaaggat tttccatctt tcatagatga tctcaatccg
      181 cctaaaaaat taagaaattc atcctcggta taatcctgga gcttttcttt aaacatattt
      241 atctcctgta gtgaatctca tcgtgtagtc taggggtaac aattcgtaaa ttatcaatat
      301 cgtatacgct gccgccatat tcaactgcaa cacgatgatg tatctggaat tttttaacta
      361 tttctttagg gccataatac caaccgtcaa taggtgcttt tgcggttaat ccttgtttta
      421 tacgtgtttg gttaccactt gaaaactgcg ctatcaactc cggatcctga ctcacctcca
      481 gccacagcgc ctcgcgaaac tcatcaaagg ttttaaactc ccgtccgcgc aatttatcag
      541 caatatgcgc cgggattggt gcgccgttgc cgttattgga taccgacata tccagccagc
      601 gagttcctgg tgaaagcagg acgccgcgcc ctgttacaac accaggcagc tttcgcggat
      661 cctccttcag gtaaacatag atgggcttaa taccggatcc cgccggaaaa accagaatat
      721 aatcgttgaa ttctttcgga tcaggaaccg ggaatggcgg cgtggtatag gtatcggtgc
      781 cgtccggaat cggcgtcacc agaatggtgg gctgctccag cggcggcctg tcgttaccgg
      841 tatggcccgg aacataccca tgttccggat tttccggcgt ccaggtaatc agggggccat
      901 cgacatcgtg agcgggggta aattcataac gctgcgtctg ctcgttccac tgcatgttcg
      961 ccacgcggac gttttcatac ggcgtacctt cacccgtatg aacaccatac acctgaggct
     1021 tgccgtgaat gtcagttccc cagaaaaaac gtacccgggt ggtggcggta ctgcctgcta
     1081 gtgctttctg ccgcagttct tcctcatacc agaactcatc acgtccgggc acgcgagcac
     1141 tgccctcgcc cgcagaggga atatacaaag cagatgccaa cgcaccgatg taagagccgc
     1201 aggttgcact gagtatgcca ctgagcgacc attttcccca tatttgagcc gccaacacac
     1261 tacccggaaa cagtagcatg gtttgagctt ctgcgggttg cccaacatcg ttagcctccg
     1321 gatggtggta tccacagcag tcatcaacca ctggcagagc aaaaatcgct acttgcccga
     1381 agttttcagc gggttcttct gccgttccgg catctgtaca tcccttctcc ttcagacagg
     1441 atttggcata gaccggttta accgctggtg gagagagtgg aaggttaaca actggtagcg
     1501 gctcagggtt aagattactg gcaggtgggt ttttaggtgg aataaataag ctattatagc
     1561 cagcaggaaa ggccaaccag tcaaattccg tattggctat tagatgtttg cataatatgt
     1621 agtcataatt aacatttaca cattcggtag gaagattatt gtcagaaatg tttacatgaa
     1681 tagcagttaa tgaagcattt cttagctgaa tataataata ccgttcccag cgcccgcttt
     1741 tatttatacg atacaaatcg atttcaataa ataaccgctc attctgattg atggcattag
     1801 acagtagcgg tgagctttta tcaatgagtt tgcaaaattg tagcccctgt agattaacgc
     1861 ctttaccagt actggtaatg gcgttggtga gtgcaaaggc aaaaatttca tcttcatggc
     1921 caacctgcca gcgattacca accgacgcat acgtcccaca gcctgaagaa atatcgccct
     1981 gcttctcacc gattattctc aggtaaa
//
LOCUS       DAZFFE010000244         1973 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZFFE010000244 DAZFFE010000000
VERSION     DAZFFE010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.246"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..590
                     /locus_tag="ACHJ69_004890"
                     /pseudo
     CDS             1..590
                     /locus_tag="ACHJ69_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008826.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(642..1010)
                     /locus_tag="ACHJ69_004891"
     CDS             complement(642..1010)
                     /locus_tag="ACHJ69_004891"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017672.1"
                     /translation="MKKIIFGMSALIMSFSSIAGGGGSWSPSISPEHCVGVSYNPFSV
                     FWTGMKQCQEAIDHGYAQGVGFSGVVSYGNGNESSNMRAFSGVVHPGERLTSNQLGVP
                     ETIGNAKINGTGKIAAFWAQ"
BASE COUNT          570 a          415 c          351 g          637 t
ORIGIN      
        1 caacattttc aatgtttcac tcttttggtt acagactcat accccgtatt cgcaacctcg
       61 gcagtcgcag gttgtacgtt atttccccct cagaggaata taaaccactt gatgccctga
      121 tcgccggaaa ggccaaaatg gaagtaatag aacagcactg ggatgaagtt ctgcggctta
      181 aggcctcgat cggcgctggt ctggtaccac catcggtaat actgaaaaag ctggcagcat
      241 ctccgagaca aaacaggctc aatctggccc tgcgtgaaat gggacgtatt gaacgctcaa
      301 tttttatctg tgactggttg cttgacactc ggttacgtcg cagatcacac acaatcctca
      361 acaaaggcga aagccgccat gcacttgccc gcgctatttt tcttcaccaa ctgggcgaat
      421 tccggaacag ggctgccgag acaatggctc acagggcatc gggcctcaac cttgttgtta
      481 atgcaataat tctgtggaac acggtctatc tcagtcgggc tgttgattac attcgcagcc
      541 aggggatcat tataccggaa gagctcttgt ccagtgtatc accactaccc taagattcaa
      601 tactgacaag agagcagact gcaatgcagc ctgctgttaa attattgtgc ccagaaggca
      661 gcaatttttc ctgtaccatt aatttttgca tttccaatag tttctggtac acctaattga
      721 tttgaagtca atctttcacc tggatgcact actccactga atgctctcat attacttgat
      781 tcattaccgt taccatatga aaccacaccg ctaaaaccta caccttgtgc ataaccgtga
      841 tcaatagcct cttggcattg tttcatgcca gtccaaaaaa cagaaaaagg attataggat
      901 acaccaacgc aatgctccgg actaatactt ggagaccatg accctcctcc cccagcaata
      961 cttgaaaaac tcatgattaa tgccgacatt ccaaatataa tctttttcat aaatagctcc
     1021 tgtttgtttt gaaattagtt tcaaattaca caatatttat ttcttttttg atctcctcct
     1081 tattcacagg tataaacata taaaaataat ccattacatt ttatacgatg tcttataatt
     1141 aaaagataaa aaaacaaaac agattgatac aatataatat tgtgtttacg gaataaacac
     1201 ttcttcagtg taatgttagt tttgactgtt taacttccta tttatctatc atgttgtaag
     1261 ttgtgggtgt ttactcatta agtattagat atgtaagtct cctgcataat agcgatattc
     1321 acatttggat atctgccagc attatatgtt ttcctctggg cgagactata tttataaaat
     1381 tcttcttgga agaaagtaga ttccgggcta tattattgta tattagtaga tatttcacag
     1441 tcaggaatgg cttatgtaga tgtaattgat aacgacatac tccgtaaaga tcaattatta
     1501 cggacaacat gcaacacagt gaccttcgat aaagataatt tcactttgga tgtttcgtga
     1561 agtatgctaa tgccattttg gataatggtt atcagcgtca ggctcatcat ttgagctttg
     1621 caatattcat tgcgaagcat ctggtgtata ttggtcagtt gcgtcttact ggatgatacc
     1681 gattttgtca cggtgcacct ctggttgaca gtttctcctt aattgcatgt ctaatatttg
     1741 ccggtatgat ctcttcatct tggtaaatcc atgttgaaaa cacatttaat tgactccttt
     1801 ctccacataa caatatagca ctactggttg acgtcaattt atattttgct gatgttttta
     1861 tgcaataaaa atattttttt caagaataat aaaaggctct actttggatc tcaaataaag
     1921 actttcatct attttccagt ctttgtttat aacgattgtt ccgccattgt ttt
//
LOCUS       DAZFFE010000245         1912 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFFE010000245 DAZFFE010000000
VERSION     DAZFFE010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.247"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            66..1088
                     /gene="istA"
                     /locus_tag="ACHJ69_004892"
     CDS             66..1088
                     /gene="istA"
                     /locus_tag="ACHJ69_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HFO5017673.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHLDENLVNFDKHPLHHPLSIYDS
                     FCRGVA"
     gene            1085..1867
                     /gene="istB"
                     /locus_tag="ACHJ69_004893"
     CDS             1085..1867
                     /gene="istB"
                     /locus_tag="ACHJ69_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFO5017674.1"
                     /translation="MMMELQHQRLMVLAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIER
                     NENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQ
                     RGVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGD
                     AALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT          500 a          495 c          491 g          426 t
ORIGIN      
        1 gatccacacc caacgcctga aatcagatcc agggggtaat ctgctctcct gattcaggag
       61 agcttatggt cacttttgag acagttatgg aaattaaaat cctgcacaag cagggaatga
      121 gtagccgggc gattgccaga gaactgggga tctcccgcaa taccgttaaa cgttatttgc
      181 aggcaaaatc tgagccgcca aaatatacgc cgcgacctgc tgttgcttca ctcctggatg
      241 aataccggga ttatattcgt caacgcatcg ccgatgctca tccttacaaa atcccggcaa
      301 cggtaatcgc tcgcgagatc agagaccagg gatatcgtgg cggaatgacc attctcaggg
      361 cattcattcg ttctctctcg gttcctcagg agcaggagcc tgccgttcgg ttcgaaactg
      421 aacccggacg acagatgcag gttgactggg gcactatgcg taatggtcgc tcaccgcttc
      481 acgtgttcgt tgctgttctc ggatacagcc gaatgctgta catcgaattc actgacaata
      541 tgcgttatga cacgctggag acctgccatc gtaatgcgtt ccgcttcttt ggtggtgtgc
      601 cgcgcgaagt gttgtatgac aatatgaaaa ctgtggttct gcaacgtgac gcatatcaga
      661 ccggtcagca ccggttccat ccttcgctgt ggcagttcgg caaggagatg ggcttctctc
      721 cccgactgtg tcgccccttc agggcacaga ctaaaggtaa ggtggaacgg atggtgcagt
      781 acacccgtaa cagtttttac atcccactaa tgactcgcct gcgcccgatg gggatcactg
      841 tcgatgttga aacagccaac cgccacggtc tgcgctggct gcacgatgtc gctaaccaac
      901 gaaagcatga aacaatccag gcacgtccct gcgatcgctg gctcgaagag cagcagtcca
      961 tgctggcact gcctccggag aaaaaagagt atgacgtgca tcttgatgaa aatctggtga
     1021 acttcgacaa acaccccctg catcatccac tctccatcta cgactcattc tgcagaggag
     1081 tggcgtgatg atggaactgc aacatcaacg actgatggtg ctcgccgggc agttgcaact
     1141 ggaaagcctt ataagcgcag cgcctgcgct gtcacaacag gcagtagacc aggaatggag
     1201 ttatatggac ttcctggagc atctgcttca tgaagaaaaa ctggcacgtc atcaacgtaa
     1261 acaggcgatg tatacccgaa tggcagcctt cccggcggtg aaaacgttcg aagagtatga
     1321 cttcacattc gccaccggag caccgcagaa gcaactccag tcgttacgct cactcagctt
     1381 catagaacgt aatgaaaata tcgtattact ggggccatca ggtgtgggga aaacccatct
     1441 ggcaatagcg atgggctatg aagcagtccg tgcaggtatc aaagttcgct tcacaacagc
     1501 agcagatctg ttacttcagt tatctacggc acaacgtcag ggccgttata aaacgacgct
     1561 tcagcgtgga gtaatggccc cccgcctgct catcattgat gaaataggct atctgccgtt
     1621 cagtcaggaa gaagcaaagc tgttcttcca ggtcatcgct aaacgttacg aaaagagcgc
     1681 aatgatcctg acatccaatc tgccgttcgg gcagtgggat caaacgttcg ccggtgatgc
     1741 agcactgacc tcagcgatgc tggaccgtat cttacaccac tcacatgtcg ttcaaatcaa
     1801 aggagaaagc tatcgactca gacagaaacg aaaggccggg gttatagcag aagctaatcc
     1861 tgagtaaaac ggtggatcaa tattgggccg ttggtggaga tataagtgga tc
//
LOCUS       DAZFFE010000246         1893 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZFFE010000246 DAZFFE010000000
VERSION     DAZFFE010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.248"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..585
                     /locus_tag="ACHJ69_004894"
     CDS             <1..585
                     /locus_tag="ACHJ69_004894"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFO5017675.1"
                     /translation="EIRRVNYSSVRNCKTGNYKDKLKNKFNLRSDIARASNEILEATS
                     LPSVNFAISFTPPETGNINKITTKRADFGASINVSIPVSQYFLRNTYQKQFAIDVARL
                     KLEYDNSLEENENETRSIERRIKSLENSIETTKKSIVVERNKINYIFSRIKEGQDNII
                     SYYQQLDTYEALQINLKEEEKELEFQKVLLYFIS"
     gene            548..1321
                     /locus_tag="ACHJ69_004895"
     CDS             548..1321
                     /locus_tag="ACHJ69_004895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017676.1"
                     /translation="MSFRRFYYILFLSSISFQSCAETYFGVLRGNEIIQYKSPYAGII
                     NLHVKNVGDVDKNKNLFEIESFEYQAKIDIINIKIRRLERNKKHLERSYEYSRDAYNS
                     GFIPLRDLQEKKDAISETELDIKELNTEKKALIHLLQSGNISMERDFIIRDIYVSQRQ
                     AVNTGDSLIKVETLNKYHIDIKFDPVAIKGRIQDKDIKLKSLVNGFSCSGNVVKITSA
                     SSDGNSSSPGLKIATIELNTPDVDMSTLLDTAFEIIVSD"
     gene            1314..>1893
                     /locus_tag="ACHJ69_004896"
     CDS             1314..>1893
                     /locus_tag="ACHJ69_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013650430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO5017677.1"
                     /translation="MINISIRSKFFSERLILKDLNINLIPGDFLVLTGPSGSGKSTLL
                     NIIGMLDTEFDGEYIIDNSIIDLNNPVNTTFLRKKYFGYVFQNSLINEKQSITRNIIS
                     SVDYNKKKEMQTRVIKTLNMVGLHDINRPTPVLSGGEKQRLALARAIIKEPEILLADE
                     PTASLDKKNKIKIINILKDFSISGGIVVMVTHD"
BASE COUNT          763 a          248 c          330 g          552 t
ORIGIN      
        1 gaaataagga gggtaaatta ttcctcagtc aggaattgta aaactggaaa ttataaagac
       61 aaactgaaga ataaatttaa tctgcgttca gacatagcgc gcgcatctaa tgaaatatta
      121 gaggctacat cattaccatc agttaatttc gcaatctcgt tcaccccacc agagacggga
      181 aacataaata aaattacaac aaaacgagca gattttggtg catctattaa tgtatctata
      241 cctgtaagtc agtatttttt aagaaataca taccagaaac aatttgcaat tgatgtagca
      301 agattaaaat tagaatatga taattctctt gaggagaatg aaaatgaaac aagatcgatt
      361 gaacgaagga taaaatcact agagaactct atagaaacaa ccaaaaaatc catagtagta
      421 gagagaaata aaattaatta tatattctca agaataaagg agggacaaga taacattata
      481 tcttattacc aacaactgga tacttacgaa gctttacaga taaatttaaa agaagaggag
      541 aaagaacttg agtttcagaa ggttttatta tattttattt cttagctcaa tatcttttca
      601 gtcctgtgca gaaacttatt ttggtgtgct caggggtaat gaaattatac aatacaagag
      661 tccttatgcg gggataataa atctgcatgt taaaaatgta ggtgatgttg ataagaacaa
      721 gaatttattc gaaatagaaa gtttcgaata ccaagctaaa attgacataa taaatataaa
      781 aataagaaga ttagaaagaa acaaaaaaca cctagaacgt tcatatgagt atagcaggga
      841 tgcgtataat tctggtttta tcccattaag agatttacag gaaaaaaaag atgctattag
      901 cgaaacagag ttagatataa aagaattaaa taccgagaaa aaagcattaa ttcatttatt
      961 gcaatcaggt aatataagca tggaaagaga ttttattata agagatatat atgtgagcca
     1021 gagacaagca gtaaataccg gtgatagttt aattaaagta gaaactctta ataagtatca
     1081 tatagatatc aaatttgatc cagttgctat taaaggtagg attcaggaca aggatataaa
     1141 attaaaatca ttagttaatg gctttagttg ttcaggaaat gtcgtaaaaa taacaagtgc
     1201 tagttctgat ggaaactcaa gttcgccagg gcttaaaata gcaactattg aattaaatac
     1261 tcctgatgtg gatatgtcaa ctctcttaga tacagcattt gagattatag taagtgatta
     1321 atatatcaat aagatctaaa tttttttctg agcgattaat tcttaaagat ctaaacatta
     1381 atctgattcc tggagatttc ttagtattga cagggccttc aggttcaggg aaaagcacac
     1441 ttctaaatat aattggcatg cttgatactg aatttgatgg agaatacatt attgataata
     1501 gtattattga tttaaataat cctgtcaaca caacgtttct caggaaaaag tattttggat
     1561 atgttttcca gaactcacta ataaatgaga aacaaagtat tacacggaat attatttctt
     1621 cagttgacta taataaaaaa aaagaaatgc agacaagagt aataaaaaca ttgaatatgg
     1681 tcggtttgca tgatatcaat agaccaaccc ccgttttgtc tggtggagag aaacaaagat
     1741 tagcactagc aagagcaata attaaagaac cagaaattct tctcgccgat gaaccaacag
     1801 caagtttaga taagaaaaac aaaataaaga taataaatat attaaaagat tttagtattt
     1861 ctggtggtat tgtcgtcatg gttacgcatg acc
//
LOCUS       DAZFFE010000247         1893 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFFE010000247 DAZFFE010000000
VERSION     DAZFFE010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.249"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            2..77
                     /locus_tag="ACHJ69_004897"
     tRNA            2..77
                     /locus_tag="ACHJ69_004897"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Thr,seq:ggt)
     gene            117..232
                     /gene="rrf"
                     /locus_tag="ACHJ69_004898"
     rRNA            117..232
                     /gene="rrf"
                     /locus_tag="ACHJ69_004898"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(295..1053)
                     /locus_tag="ACHJ69_004899"
     CDS             complement(295..1053)
                     /locus_tag="ACHJ69_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO5017678.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLIVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAQKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPNEFFAHPKSERTRAFLSQVIH"
     gene            complement(1061..>1893)
                     /locus_tag="ACHJ69_004900"
     CDS             complement(1061..>1893)
                     /locus_tag="ACHJ69_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO5017679.1"
                     /translation="YPHDQRWRINLALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPL
                     VVWWLMYGGFLGLDRVETRQWGGLTLTLIIASVGIAGALPWGILLALGRRSHMPIVRI
                     LSVIFIEFWRGVPLITVLFMSSVMLPLFMAEGTSIDKLIRALVGVILFQSAYVAEVVR
                     GGLQALPKGQYEAAESLALGYWKTQGLVILPQALKLVIPGLVNTIIALFKDTSLVIII
                     GLFDLFSSVQQATVDPAWLGMSTEGYVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH
                     "
BASE COUNT          513 a          516 c          429 g          435 t
ORIGIN      
        1 tgctgatatg gctcagttgg tagagcgcac ccttggtaag ggtgaggtcc ccagttcgac
       61 tctgggtatc agcaccactt tttaggttaa agttcggcag attagaaaag aatttgtctg
      121 gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta
      181 gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag acatcaaatc
      241 aagcgaaagg ccatccgaaa ggatggcctt tttgcttttc gaactaacat tcaattaatg
      301 gattacctgc gataaaaatg ccctcgtacg ctctgattta ggatgcgcaa aaaattcatt
      361 aggtgcagct tgctccacta tttccccacg atccataaaa atcacccggt cagcgacggt
      421 tcgtgcaaac cccatctcat gtgttacaca caacattgtc atacccgact gcgccagccc
      481 aatcatcgta tccagcacct ctttcaccat ctcaggatcg agcgccgacg ttggctcatc
      541 aaacaacata attttcggct tcatacacag cgaacgtgct atggcaacac gttgctgctg
      601 accaccagaa atctgtccgg gaaacttctg ggcatgttcg gcaattctca cccgctcaag
      661 gtaatgcatc gccagagctt cagcctcttt ctttggcatc ttgcgtaccc aaatcggtgc
      721 cagggtacag ttctgcaaca cggtcagatg agggaagaga ttgaaatgct gaaagaccat
      781 tcccacttcc tgcctgacgc gctcaatatt gcggatatct tcattaagtt cgatgccatc
      841 taccacgatc cgtccctgtt gatgttcttc cagatgatta atacaacgaa tggttgtcga
      901 tttaccggaa cctgaagggc cacacagaac gatccgttcg cccggttgca cgattaaatt
      961 tatatttttc aaaacatgga attgtccata ccatttattg acgttttcca gcgtaatcat
     1021 cgcgttagca ggttgcagta aaatttggct catagtgtcc tcaatgcggt gtacgcccgg
     1081 tgttaaaacg tttttccaga tgttggctat aacgtgacat gctgaaacag aagatccagt
     1141 agatcagcgc ggcgaaaaca tacccttccg tcgacatacc caaccaggcg ggatcaacgg
     1201 ttgcctgctg aacgctactg aaaagatcga acaacccgat gatgatcacc agactggtat
     1261 ctttgaagag tgcgatgatg gtatttacca gcccaggaat taccagcttc aacgcctgtg
     1321 gcagaataac cagcccctga gttttccagt aacccaatgc cagcgactct gccgcttcat
     1381 attgcccttt aggcagcgcc tgcaatccgc ctcgcacgac ttccgcaaca tatgctgact
     1441 gaaataggat cacgccaacc agcgcgcgga tcagtttgtc gatactggtg ccttctgcca
     1501 taaacaacgg cagcatgacc gaagacataa acaatacggt aatcagcggt acgccgcgcc
     1561 agaactcgat aaaaatgacc gataagatac gcacaatcgg catatgggag cggcgaccta
     1621 acgccagtaa gatcccccac ggtagcgccc ccgcaatccc aactgatgca ataattaaag
     1681 ttagcgtcaa cccgccccat tgccgggttt caacccgatc aagaccaaga aacccgccat
     1741 acatcagcca ccagacaacc agtgggtaaa tcaccgccca gaccgcaata tagcgaccgc
     1801 gatgcggaag tattttccag aacattggtg cgatagacac aaggccaatc agtaatgcca
     1861 ggttaattcg ccagcgttgg tcgtgtgggt aaa
//
LOCUS       DAZFFE010000248         1828 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZFFE010000248 DAZFFE010000000
VERSION     DAZFFE010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.250"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1727)
                     /gene="yciQ"
                     /locus_tag="ACHJ69_004901"
     CDS             complement(<1..1727)
                     /gene="yciQ"
                     /locus_tag="ACHJ69_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFO5017680.1"
                     /translation="MIVGLFFSSLSYAKNTEIPSYEEGISLFDVEATLQPNGVLDIKE
                     NIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEPWHLDWHIGLM
                     SIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNNWPFEIYKTRFSLE
                     LPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVLYRWPHALLGN
                     APAPQTTNIFSHLLLPSTSSLLIWFPCLFLACGWLYLWKRRPQFTPVNVIETDVIPPD
                     YTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLKDQYDKNQQILIRVNEGATRNNAV
                     LLPAEQLLLEALFRKGDKVVLTGRRNRVLRKAFLRMQKFYLPRKKYSFYRPDAFLQWG
                     GLAILAVILYGNLSPVGWAGMSLVGDMFIMICWIIPFLFCSLELLFARDDDKPCVNRV
                     IITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGMGYIFLPKFTQ
                     TGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAVSANLGREWAARIT
                     ASLTAAVRAP"
BASE COUNT          528 a          451 c          401 g          448 t
ORIGIN      
        1 ggggcgcgaa cagccgctgt aagtgatgcg gtgatacgtg ctgcccattc tctgccaagg
       61 tttgccgaga ccgcccaacc taacaatgcg taatccagtt tccgtgtttc ccctttccgc
      121 cgccgcccac tgtgtgttgc cgcctcttta cgcgcaagat agttaacgat agcttcaccg
      181 tgggcataac gttgctgccc agtttgggta aactttggca gaaaaatata ccccatgcca
      241 gtgagataac cggtcaggaa aacagcgcta aaataacccg ctggcatata ccagtaaaag
      301 aatacatctc cgacattgat atagagagag taaaaggcca cgcctgaaca aatcagcggt
      361 aaaaacaaag tgatgattac acgattaacg caaggcttgt catcatcgcg ggcaaacaaa
      421 agctcaaggg aacaaaataa aaaaggaata atccagcaga tcataataaa catatcgcct
      481 accagactca ttcctgccca acctacggga ctcaggttac cgtagagaat gaccgccaat
      541 attgccagtc caccccattg caaaaacgca tcaggtcggt aaaacgaata ctttttacgc
      601 ggcagataaa atttctgcat ccgtaaaaat gccttgcgta agactctgtt gcgtctcccc
      661 gtaagaacga ccttatcgcc tttacgaaat aacgcttcca gtaataactg ctctgcgggc
      721 agtaataccg cattatttct ggtggcgcct tcattaacac gaatcaggat ttgctggttc
      781 ttgtcatact gatctttcag atgaattttt cccttcacga tcagatttac gatatcggca
      841 caaaaacctt tatcgtcgta aaccagcttc gcatcgagac gtaacatgcc gggcgtgtaa
      901 tctggcggaa tgacatcggt ttcaatcaca tttaccggcg taaattgcgg cctgcgcttc
      961 cagagatata accatccaca cgccaggaac agacacggaa accaaattaa caacgatgac
     1021 gtagagggta aaagaagatg gctgaaaata ttcgtcgttt gtggtgccgg ggcattacca
     1081 agtaaagcgt gaggccagcg gtagaggacc gtgaagtctt cacgataaaa cggatcgcgg
     1141 gattcaattc ttccgtcctc aaggatgcgg ccatttcgat atgtgtcgcc ctcttctcca
     1201 gtaaagagat caatttcgct aaatggatta cccgcaatat caggcaactc gagtgaaaat
     1261 cgggtcttat agatttcaaa cggccagtta ttaccagtca cgttccagat tagcagatca
     1321 gaatccccct cacgcaggaa agcattttta acctgataat gaatttgata atgatagtcg
     1381 ccttgaggca agaaacgttg tttatcgccc acgacaatac tcattaaccc gatatgccag
     1441 tcaagatgcc agggttcagg aataccatca cgggtgacgc caacaatatg atagttcagc
     1501 agtgcagcgt ccccatcagg ctgcatccag agtcgcggta aatcacgata aaatccgtgc
     1561 ttaatctgct gatttcgcgc ctgaaaatga atgttctctt tgatgtcgag caccccattc
     1621 ggttgcagag tggcttcaac atcaaagagc gagatccctt cttcataaga agggatctcc
     1681 gtgtttttcg cataactcaa cgaagagaaa aaaagcccaa ctatcaggtg atgctgccaa
     1741 cttactgatt tagtgtatga tggtgttttt gaggtgctcc agtggcttct gtttctatca
     1801 gctgtccctc ctgttcagct actgacgg
//
LOCUS       DAZFFE010000249         1758 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZFFE010000249 DAZFFE010000000
VERSION     DAZFFE010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.251"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1171)
                     /locus_tag="ACHJ69_004902"
     CDS             complement(<1..1171)
                     /locus_tag="ACHJ69_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017681.1"
                     /translation="MAYSDEQRPEAQFGNQNRNSLNIQQPGETDSYDAFFSDPNRWKD
                     NSTSFSLGDVLPTMGKGVAQSVRGAGDMARGLGDAMIQSPVKTGARILNEFSRMGLPG
                     VATVQDIFVGGSKGADEVIDTLPDGKNAVTDTVGKGLKVTGKAVSGGAKASDEWLTGK
                     MSPGAQRALNTPMTEGYDDSAVWVAKGVNLIGALVPDMVAGGVTKKLGEVTLRKVLTA
                     GLEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSSASAQGQSAMAAAGSVLN
                     ADYSELAKSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAV
                     NAMAAKLGDAQLFNLAMRGTAKTVKSGIVRNATEQGTINAAQGGYSRYQENTALRE"
     gene            complement(1240..>1758)
                     /locus_tag="ACHJ69_004903"
     CDS             complement(1240..>1758)
                     /locus_tag="ACHJ69_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017682.1"
                     /translation="TEYGSAHPDDQTVLAVPVDKAVELVRDRSKFAEISKNFGYFTPK
                     QQGLSLAKLQEGASSVAAEAIKNGGNAQAAVDEYYAATGSPQHQQKIQQQKIQQGITS
                     WAGDDPDKQAFAREVASRQPEMLEPQNQKLLENGYANFLRIQKARGEQARDDSAASAS
                     EFIRGLKQNYAQ"
BASE COUNT          370 a          543 c          399 g          446 t
ORIGIN      
        1 tctcacgcaa tgccgtgttt tcctgatagc gtgaatagcc accctgcgcc gcattaatcg
       61 tcccctgttc cgtggcattt ctgacaatgc cgcttttaac ggtcttcgcc gtgccccgca
      121 tcgccagatt aaacaactga gcatcgccca gttttgccgc catggcattc acagccagca
      181 attcaggatc ggttgccagc tgcgcgcgca cctcatcggc aacacgctct tttgccagat
      241 ccattttctg gcgatcagta agctgtgcgt gctgcggatc tgcatcaatt gacaaaaacg
      301 tctgctgaaa tttcggggac ttcgccagct cagagtaatc cgcgttaaga acagagcctg
      361 ctgctgccat tgcactctgc ccctgtgcgc tggctgagga atgggtgatc aggcctgcct
      421 ggaataaatc cggcattttt ttatcgacag cttctgctgc cagtgcggta gctctttccg
      481 gctgcatccc tgccgcaatg tattttttct ccagcccggc ggtcagcact tttcgcagag
      541 tgacttcacc cagcttttta gtcacaccgc ctgcaaccat atccggtaca agcgcaccaa
      601 tcaggtttac gcccttcgcc acccagaccg cagaatcatc atatccttca gtcatcggtg
      661 tattcagcgc ccgctgcgcc cccggcgaca tcttgccagt cagccattca tccgaggctt
      721 tagcgccgcc actgactgcc ttgcctgtga ccttcagacc tttaccgaca gtatcagtaa
      781 ccgcattttt tccatcaggc agagtgtcga tgacctcatc cgcccccttg ctgccaccga
      841 caaaaatatc ctgaacagtt gcgactcccg gcagccccat acggctgaac tcatttaaaa
      901 tacgcgcccc tgtttttacc gggctctgaa tcatcgcatc accgagtcca cgggccatgt
      961 cccctgcccc ccggacggac tgggcgacac ctttccccat agttggcaat acatcgccca
     1021 ggctgaacga cgtgctgttg tccttccagc gattgggatc ggagaaaaac gcatcatagc
     1081 tgtcagtctc gcccggctgc tgaatgttca ggctgttgcg gttctggttg ccaaattgcg
     1141 cttcagggcg ctgttcatcg gaataagcca tacagactcc ataaaaaaac ccggcacaat
     1201 gaccgggtat caggaaatag tattaacggg aatggtgaat tactgcgcgt aattctgttt
     1261 cagaccacgg ataaactcag atgcagaggc cgcactatca tctctggcct gttcccccct
     1321 ggccttttga atacggagaa aattcgcata cccgttctcc agcaatttct gattctgagg
     1381 ctccagcatt tctggctgac gggaagcaac ttctctggcg aatgcctgct tatccggatc
     1441 atcacccgcc caactggtaa tcccttgttg aattttttgc tgctgaattt tctgctgatg
     1501 ttgcggtgaa ccagttgcag cgtaatactc atccaccgcc gcctgagcat tcccgccgtt
     1561 cttgatcgct tccgccgcta cactgctggc tccttcctga agtttcgcca gagaaagtcc
     1621 ctgctgcttc ggcgtgaaat agccgaaatt tttcgaaatt tccgcgaatt tactgcgatc
     1681 cctgacaagc tcaacagcct tatcaacagg caccgccagc acggtttgat catcaggatg
     1741 tgcactgccg tattccgt
//
LOCUS       DAZFFE010000250         1749 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFFE010000250 DAZFFE010000000
VERSION     DAZFFE010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.252"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..183
                     /locus_tag="ACHJ69_004904"
     CDS             <1..183
                     /locus_tag="ACHJ69_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017683.1"
                     /translation="AIKLAGDFYSMMGYTHRPGFKYWESPHPQEQLVFQMACRAFEVI
                     RGSDVMDAVADLEDEE"
     gene            180..929
                     /locus_tag="ACHJ69_004905"
     CDS             180..929
                     /locus_tag="ACHJ69_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5017684.1"
                     /translation="MSEINYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDAQAYANAEFIAEANPATVLALLDERERNLQYIKSRDQEN
                     EDIALTVGRLRVELEGKDSKIANLTAERDALREGEMGDARHSNTRAAADIYFQLVEEC
                     EIPAGGSLVEYVDDMRENLEAAEKRIAELESGSQAQKLVEAIIVAIENEQERLFDEDY
                     LMDSKECIDVIREEVKRWNDSRAAGIRIKGE"
     gene            931..1254
                     /locus_tag="ACHJ69_004906"
     CDS             931..1254
                     /locus_tag="ACHJ69_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HFO5017685.1"
                     /translation="MTTSHSAITQKKAFHILERLEALATEEEISPEKLVEFSRVILRR
                     KNDMERLTSGAPSLSVRRTLCCSFCNKSQYAVKKLIAGDAVFICDECVDVCNRIIRGE
                     KEGSA"
     gene            1251..>1749
                     /locus_tag="ACHJ69_004907"
     CDS             1251..>1749
                     /locus_tag="ACHJ69_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017686.1"
                     /translation="MKFSKFSELVNRILSNNHSHRRDMDVTIVVHSPGRIGSTPSVEV
                     QSIQAGFDWDAGKVMIFPAQPLTTLTPEQVADITDSVRKGQSWHAYQEYKKHKEQLEK
                     LSMELEAAKQREKDLFMENVRLKSGIAGLIHLGIRYADVEVMKIAGDAQLSTPCTDSI
                     INSIAT"
BASE COUNT          497 a          368 c          474 g          410 t
ORIGIN      
        1 gcgattaagc ttgctggtga tttttattca atgatggggt acacgcatcg cccaggcttc
       61 aaatattggg agtctcctca cccgcaagag caactggtat ttcaaatggc ctgccgtgct
      121 tttgaggtta ttcgcggttc tgatgtgatg gacgccgttg ccgacttgga ggatgaagag
      181 tgagcgagat taactatcag gcactgcgtg aaaaggcaga gaaagcaact aaaggaagct
      241 acatcgtagg gcatacatct gttaaccaac acggcaattt aacaggagtt tttgtttgtc
      301 aaaaatggaa aggagaaccc ggtggcgtaa ttgcagaatg tcacgttaac tgcctggttg
      361 aaacagatgc tcaggcttat gcaaacgctg aattcatagc agaggctaac ccggctaccg
      421 tgctggcact gctggatgaa cgggaaagaa acctgcaata catcaaaagc cgcgatcagg
      481 agaacgagga tattgcgctt acggttggga ggctgcgcgt tgagctggaa ggcaaagaca
      541 gcaaaatagc caatcttacc gccgaacgcg atgctcttcg tgaaggtgag atgggcgacg
      601 ctaggcatag caacacacgg gccgcagctg atatctactt ccaactggtc gaggaatgcg
      661 aaattcctgc tggcggatct ctggtcgagt acgttgacga tatgcgcgag aatctggaag
      721 ccgcagagaa gcgcattgca gagttagaaa gtggttctca ggcacaaaag ttagttgaag
      781 caatcattgt tgcgatagaa aacgaacagg aacgtctttt tgatgaagat tacctaatgg
      841 attcgaaaga atgcattgac gtaattcgtg aagaagtaaa gcgatggaat gattcccgcg
      901 ccgctggcat tcgcatcaaa ggagagtgag atgaccacat cgcattctgc aattacccag
      961 aaaaaagcct tccacatact cgaacgatta gaggcgcttg ctacggagga ggagatatcc
     1021 ccggagaaac tggttgagtt cagccgtgtg atattgcgtc gcaagaacga tatggagcgg
     1081 ctgacatctg gcgctccatc cttatcagtc aggcgaacac tttgttgcag cttctgcaac
     1141 aaatcccagt acgccgtcaa aaagttaatt gctggggacg ccgttttcat ctgcgacgag
     1201 tgtgtggatg tgtgcaacag aattatccgg ggagaaaaag agggatcagc atgaaatttt
     1261 ccaaattttc tgagttggtg aatcgtattt tgtccaacaa ccacagccat cgtcgcgata
     1321 tggatgtaac gatcgttgtt cattcgcctg gtcgcatcgg ttcaacacca tcagttgagg
     1381 ttcagtcaat tcaggcgggt tttgattggg atgccgggaa ggtgatgatt tttccagcac
     1441 agccactgac cacgctaaca ccggagcagg ttgctgatat cactgatagt gtgcgcaaag
     1501 gtcagtcttg gcacgcgtat caggaataca agaagcataa agagcagttg gaaaaattgt
     1561 cgatggagtt ggaagccgct aaacagcggg aaaaagatct gtttatggaa aatgttcgac
     1621 ttaagtcagg catagccggt ctgatacacc tcggtattcg atatgcagat gttgaggtca
     1681 tgaaaattgc tggagatgcc cagctttcta ccccatgcac tgacagcatc ataaacagca
     1741 ttgcaacag
//
LOCUS       DAZFFE010000251         1725 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFFE010000251 DAZFFE010000000
VERSION     DAZFFE010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.253"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            230..>1725
                     /gene="tssI"
                     /locus_tag="ACHJ69_004908"
     CDS             230..>1725
                     /gene="tssI"
                     /locus_tag="ACHJ69_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFO5017687.1"
                     /translation="MSLKGLRFTLEVDGQEPDTFAVVNFRLIQNQSYPFVMSVDVASD
                     SFMQTAEMLLEKKATLTIWQGVIPQRYVTGVVAGFGMQENNGWQMRYHLCIEPPLWRC
                     GLRQNFRIFQQQDIRTISATLLNENGVTEWTPLFYEDHPARKFCVQYGESDLAFLARL
                     WAEEGIFFFERFAADSPEQKLTLCDDVAGLSQAGEFPFNPDASTGAETECVSMFRYEA
                     HVRPSSVQSQDYTFKVPDWPGMYEQQGESLNGQLEQYEIFDYPGRFKDEQHGKDFTLY
                     QMESLRSDAEKATGQSNSPKLWPGTRFTLTGHPQKMLNREWQVVQSILSGDQPQALHG
                     SQGRGTTLGNQLEVIPADRTWRPRQQSKPKVDGPQSAIVTGPAGEEIFCDEHGRVRVK
                     FHWDRYNPATEASSCWVRVSQAWAGPGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRT
                     YHEDNRSPGDLPGTKTQMTIRSKTYKGSGFNELRFEDATDKEQVYIHA"
BASE COUNT          393 a          383 c          601 g          348 t
ORIGIN      
        1 gaagcataaa tctgagtcaa caacatcctg cctccgtgca ggatgttgtt tttgtacgtg
       61 taaaaaatga gtgtcagtaa ggagttacgc tttcatgagg gagatggtgg atgccggaaa
      121 cccgggcggt agcgtacctg aaaatgtaaa gatgagtgta acgctgcact ggcacccttt
      181 gctgcatggt gggaatcatt tggtccgtga atgaatcagg gaggtcatta tgtccttaaa
      241 aggtcttcgc tttacgctgg aggttgacgg ccaggagccg gacacctttg cagtggtgaa
      301 tttcaggtta atccagaacc agtcttatcc gtttgtgatg agtgtggatg tcgccagcga
      361 ttcttttatg cagacggcgg agatgctgct ggagaagaag gcaacgctaa ccatctggca
      421 gggcgtcatc ccgcagcgtt atgtcacagg cgtggtggcg ggctttggca tgcaggagaa
      481 caatggctgg cagatgcgtt accacctgtg tattgagccg ccgttatggc ggtgcgggct
      541 gcggcagaac ttccgcatct tccagcagca ggatatccgg acgatatcgg ccacgttact
      601 gaacgagaac ggcgtcacgg agtggacgcc gctgttttat gaggaccatc cggcgcggaa
      661 gttctgtgtc cagtacgggg aaagcgacct ggcgtttctg gcgcggctgt gggcggagga
      721 ggggattttc ttctttgagc ggtttgcggc ggacagtccg gagcagaagc tgacgctgtg
      781 cgacgatgtg gcggggctgt cacaggcggg agagtttccg tttaacccgg acgcatcgac
      841 aggagcggag acggagtgtg tcagtatgtt ccgttatgag gcgcatgtcc gcccgtcgtc
      901 ggtgcagagc caggattaca cgtttaaggt gccggactgg ccggggatgt atgagcagca
      961 gggtgagagc ctgaacggac agcttgaaca gtatgaaata tttgattatc cgggaaggtt
     1021 caaggatgag cagcacggca aggatttcac gctgtaccag atggagagcc tgcgcagtga
     1081 tgcggaaaag gccacagggc agagtaattc gccgaagctg tggccgggaa cgcggtttac
     1141 gctgacgggg catccgcaga agatgctgaa ccgtgaatgg caggtggtac agagcattct
     1201 gtcaggagac cagccgcagg cgcttcacgg cagccagggg agaggaacca cgctgggcaa
     1261 tcagttagag gtgataccgg cggaccggac gtggcgtcca cggcagcaga gcaaaccgaa
     1321 ggtggacggg ccgcagagtg cgatagtgac ggggccagcg ggagaggaga ttttctgcga
     1381 cgaacacggg cgggtacgtg tgaagtttca ctgggacagg tataacccgg caacggaggc
     1441 gagttcgtgc tgggttaggg tatcgcaggc gtgggcgggg ccggggtttg gtaacctggc
     1501 gataccgcgc gtgggccagg aggtgattgt tgacttcctg aacggggacc cggaccagcc
     1561 gataatcatg ggcaggacgt accatgagga caaccgttca ccaggggacc tgccggggac
     1621 gaagacgcag atgacgatac ggtcgaagac ctataaagga agcggtttta atgagttgcg
     1681 ctttgaggat gcgacggata aggaacaggt ctatatccac gcgca
//
LOCUS       DAZFFE010000252         1718 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZFFE010000252 DAZFFE010000000
VERSION     DAZFFE010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.254"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..179
                     /locus_tag="ACHJ69_004909"
     CDS             <1..179
                     /locus_tag="ACHJ69_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO5017688.1"
                     /translation="KLTRIVVHVDLQPIADELHGDYINDKSFKRHFQQWLNSLWQEKD
                     RLLTSLMSSQRQEK"
     gene            complement(220..1650)
                     /gene="yihF"
                     /locus_tag="ACHJ69_004910"
     CDS             complement(220..1650)
                     /gene="yihF"
                     /locus_tag="ACHJ69_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO5017689.1"
                     /translation="MIRKSATGAIVALAVIWGGGTWYTGTQIQPGVEKFIKGFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKIVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGPFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDDSSLSNLDIEGKVEDIVLQFSPMNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQVNIINQGEVVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDMK
                     LPLNVATETAKQLNLSEGMDAEKAQKRADKQISGMMTLGLMLQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT          474 a          382 c          338 g          524 t
ORIGIN      
        1 gtaaactgac gcgaattgtc gtacatgtgg atttacagcc tattgccgat gagttacatg
       61 gcgactacat caatgacaaa agctttaagc gccattttca gcaatggctg aactcattgt
      121 ggcaggaaaa agaccggctt ctgacatcgt tgatgtcatc acagcggcag gaaaagtaag
      181 ttttactcgc ctctgaatga gcagaggcga gtgagtattt taatgaacaa aacgtccggc
      241 acgagacata aattcttctt cgctcatctc ctgtccgtta aaaacaactt tacccggtgt
      301 ataacgcagt tgcagcgagg cggtattgtt gtcaatcgtg attaactgta acatcagacc
      361 taatgtcatc atcccactga tttgcttatc agcccgcttt tgcgcttttt ccgcatccat
      421 cccttcagat aaattaagct gttttgcggt ttctgtggcg acattaagcg gtaacttcat
      481 atcaaaattg agcgatttaa tatctttgtt tgtggatgat gaagatttgg ctgggtcggc
      541 aatactgata tccagattgg catttagttc gccgagtgcg ttcttccatc ttactggttg
      601 tttaatggtc ggctcacttt tttgtaataa cggtaaatac tctttgaaca atgcagcatt
      661 cacttcttgc agagcaactt catcgtttgc taactcaggg tgtgcagcaa gctgcttctg
      721 catcgcaatg ttatactgga tgataaattg gcgcaccgct gagggatcaa tagattcagc
      781 aatcaatgac cactcaccac taccgagttg ctgattccct tttgttaact tatcaagttc
      841 gtaggtcaga ttgacattga tataatcttt gtctttaacg cgatccagcc tggtttttgc
      901 aacgaaagca tcgatttggg caacgacttc cccctggttg ataatattaa cctgattaaa
      961 tttcgactcg ctttcaccaa ccggaaattt cttttcttct aatcgtgtca gagaatcaat
     1021 ggtaaaactt ttcgccgtta ctttgttcat tggtgaaaat tgcagaacaa tatcttcgac
     1081 tttgccttca atatccaggt tagacaatga actatcatca ccattaaaag taaactggcc
     1141 ttcaccaaaa gaaaactttc catactctgc aggggcaaca tctaatgtcg tggagaatgg
     1201 gccaccaaac gcgaatcgca atgtcgcttc cacgggcgat ttatttttcg cggcgataaa
     1261 tagcggttgt gtcagctcat tattcactaa gttcactttt gccgctgcca gtgctgggat
     1321 gacattacca tgcattaaca ttgtgatggg caacggaccg tgctcaacat ccacatcaaa
     1381 tacaattttc tggcctggct tgatattgag atcgggtgca ccgttatcaa atgtcatttg
     1441 catttgaaaa cgggaattaa aaaagccttt gtcaaaattt ttataactta acgtcatatc
     1501 gtaggcatgt tcacctttct ttttagcatc gttaaaacct ttaataaact tttcgacacc
     1561 aggctgaatt tgcgtacctg tgtaccatgt gccaccaccc cagattacgg ctaacgcaac
     1621 aatggcacct gtagctgact tacgtatcat ataaatattc cctgataaaa cagcaaaact
     1681 ttgaatatcc acttatgccg aaataatgtc cagcagca
//
LOCUS       DAZFFE010000253         1661 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFFE010000253 DAZFFE010000000
VERSION     DAZFFE010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.255"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            8..208
                     /locus_tag="ACHJ69_004911"
     CDS             8..208
                     /locus_tag="ACHJ69_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO5017690.1"
                     /translation="MEQRITLKDYAIRFGQTKTAKDLGVYQSAINKAIHAGRKIFLTI
                     NADGSVYAEEIKPFPSNKKTTA"
     gene            347..643
                     /locus_tag="ACHJ69_004912"
     CDS             347..643
                     /locus_tag="ACHJ69_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO5017691.1"
                     /translation="MEQTSYSKLSQRDIDRAETDLLINLSALTQRGLAKMIGCHESKV
                     SRTDWRYIAAILCAFQMASDISPISRAFQHAINVHANKKRPVGAGRSEQILMNI"
     gene            658..876
                     /locus_tag="ACHJ69_004913"
     CDS             658..876
                     /locus_tag="ACHJ69_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017692.1"
                     /translation="MEQYTGIILPHLEEFRQQQHQPQQPEADWVNPEIPGPSVKMCSH
                     TNVQSHQPQQPEADWVNPGDTGTVCGME"
     gene            897..>1661
                     /locus_tag="ACHJ69_004914"
     CDS             897..>1661
                     /locus_tag="ACHJ69_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5017693.1"
                     /translation="MRNYATISPQFWLGETGRKLRKSGPECMVVALYMMTSPHSNMLG
                     LYYLPVLYIAHETGLDPEGASKGLQMACEAGFCSYDHDSEVVWVHEMAAWQVGESLKP
                     GDNRCAGVRNEYSALLENPFLSLFYDKYKDDFHLDVRRESCRKIEAPSEPLSSQEQEQ
                     EQEQERDKTLLVHGEKIATDPQGDSCPVLTERPGPAGTIPEADSGRCVQQVLLVEPEQ
                     QHQPQQPEADSAMSGKPIGLTRERPGPSAGRVDYPDV"
BASE COUNT          470 a          384 c          427 g          380 t
ORIGIN      
        1 aggttgtatg gaacaacgca taaccctgaa agattatgca atacgctttg ggcaaaccaa
       61 gacggctaaa gatctcggcg tatatcaaag cgcgattaac aaggccattc atgcaggccg
      121 aaagattttt ttaactataa acgctgatgg aagcgtttat gcggaagaaa taaagccctt
      181 cccaagtaac aaaaaaacaa ctgcataagt aacaccgcta ttttcacaat ggacattcgt
      241 cctacgtcgc tgacaaagcg agccccaaga tatctgacca actaaggcca tatgcgtttc
      301 cacgcatacc tttcaactag ctattcacta ttggaaatct taagaaatgg aacaaacaag
      361 ttacagcaaa ctgtcacagc gcgatatcga tcgcgctgaa acggatttac ttatcaacct
      421 gtcagctctg acgcaaaggg gactggcgaa gatgattggc tgtcatgaat cgaaggtcag
      481 tcgtaccgac tggcgataca tcgcggcgat tttatgcgcg tttcagatgg catctgatat
      541 cagtccgatc agccgggctt tccagcatgc cattaacgtt catgcaaata aaaaacgtcc
      601 ggttggggcc ggacgttctg agcaaatcct gatgaatatc tgatattcag gcagggcatg
      661 gagcaataca cgggaataat tctgccacat ctggaagaat ttcgccagca acaacaccaa
      721 ccgcagcagc ctgaagccga ttgggttaac ccggagatac cgggaccgtc tgtgaagatg
      781 tgcagtcaca ccaatgtgca gtcacaccaa ccgcagcagc ctgaagccga ttgggttaac
      841 ccgggagata ccgggaccgt ctgcggtatg gagtaaatct tgtatgcgag gggactatgc
      901 gtaattacgc aacaatttca cctcagttct ggttaggcga aacagggaga aaactaagga
      961 agtctggtcc ggaatgtatg gtagtggcgt tgtatatgat gacctcgcct cattccaata
     1021 tgctgggcct ttattacctg cctgttttgt acattgctca cgaaaccgga cttgatcctg
     1081 aaggggcttc taaggggctt caaatggctt gcgaggctgg tttttgcagc tatgaccatg
     1141 attctgaggt tgtatgggtg catgaaatgg cagcatggca ggttggtgaa tcgctgaaac
     1201 ctggcgataa ccgttgtgct ggggtaagaa atgaatattc cgcgttgctg gaaaatcctt
     1261 ttttatcatt attttacgat aaatataagg atgatttcca tctggatgtc agacgtgagt
     1321 catgtcggaa aattgaagcc ccttcagagc ccctttcaag ccaagaacag gaacaggaac
     1381 aggaacaaga aagggataaa acccttctgg tccatggcga aaaaatcgcc acggacccac
     1441 agggggattc ttgccctgtt ctgactgaac gaccaggacc agctggcacg ataccggaag
     1501 cagattccgg tcgttgtgtg cagcaggtgc tgctcgtcga accggagcaa caacaccaac
     1561 cgcagcagcc tgaagccgat tccgcgatga gcgggaagcc gattgggtta acccgggaga
     1621 gaccgggacc gtctgcggga cgggttgatt atcctgacgt g
//
LOCUS       DAZFFE010000254         1646 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFFE010000254 DAZFFE010000000
VERSION     DAZFFE010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.257"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..69
                     /locus_tag="ACHJ69_004915"
     CDS             <1..69
                     /locus_tag="ACHJ69_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017694.1"
                     /translation="CCRFNWSTQQLCENMGLRRFFE"
     gene            66..1217
                     /locus_tag="ACHJ69_004916"
     CDS             66..1217
                     /locus_tag="ACHJ69_004916"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HFO5017695.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVSSTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALT
                     DKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIR
                     QYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
     gene            complement(1227..1487)
                     /locus_tag="ACHJ69_004917"
                     /pseudo
     CDS             complement(1227..1487)
                     /locus_tag="ACHJ69_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          496 a          366 c          384 g          400 t
ORIGIN      
        1 tgttgtagat tcaattggtc aacgcaacag ttatgtgaaa acatggggtt gcggaggttt
       61 tttgaatgag acgaacattt acagcagagg aaaaagcctc tgtttttgaa ctatggaaga
      121 acggaacagg cttcagtgaa atagcgaata tcctgggttc aaaacccgga acgatcttca
      181 ctatgttaag ggatactggc ggcataaaac cccatgagcg taagcgggct gtagctcacc
      241 tgacactgtc tgagcgcgag gagatacgag ctggtttgtc agccaaaatg agcattcgtg
      301 cgatagctac tgcgctgaat cgcagtcctt cgacgatctc acgtgaagtt cagcgtaatc
      361 ggggcagacg ctattacaaa gctgttgatg ctaataaccg agccaacaga atggcgaaaa
      421 ggccaaaacc gtgcttactg gatcaaaatt taccattgcg aaagcttgtt ctggaaaagc
      481 tggagatgaa atggtctcca gagcaaatat caggatggtt aaggcgaaca aaaccacgtc
      541 aaaaaacgct gcgaatatca cctgagacaa tttataaaac gctgtacttt cgtagccgtg
      601 aagcgctaca ccacctgaat atacagcatc tgcgacggtc gcatagcctt cgccatggca
      661 ggcgtcatac ccgcaaaggc gaaagaggta cgattaacat agtgaacgga acaccaattc
      721 acgaacgttc ccgaaatatc gataacagac gctctctggg gcattgggag ggcgatttag
      781 tctcaagtac aaaaaactct catatagcca cacttgtaga ccgaaaatca cgttatacga
      841 tcatccttag actcaggggc aaagattctg tctcagtaaa tcaggctctt accgacaaat
      901 tcctgagttt accgtcagaa ctcagaaaat cactgacatg ggacagagga atggaactgg
      961 ccagacatct agaatttact gtcagcaccg gcgttaaagt ttacttctgc gatcctcaga
     1021 gtccttggca gcggggaaca aatgagaaca caaatgggct aattcggcag tactttccta
     1081 aaaagacatg tcttgcccaa tatactcaac atgaactaga tctggttgct gctcagctaa
     1141 acaacagacc gagaaagaca ctgaagttca aaacaccgaa agagataatt gaaaggggtg
     1201 ttgcattgac agattgaatc tacagttgct ttttttaata tatctcgctc aaggcgagct
     1261 tcatttaacg ccttacgcag ttgcagaatt tcagattcca gttcagccac cgtgcgggaa
     1321 ccaggagtac cgagcccttt tctggcggcg gtaacccatt gtcctaaagt gccttcagga
     1381 agagataatc gggaagcgcc ttcactgatc gaaagttgat tttcaagaac cgttctgaca
     1441 gcttcggctt tgaactcttt agagtaacgt tgggtttttc tgctcattat tagctccttc
     1501 tgatgccatt ctatttcagg aaggagtgtc cgttaaactc aggctacctc aatatgcctg
     1561 gcggttattg cagaatctgt ttcacaataa aaaaacgatt gctgtaatcc tctattgacg
     1621 aaagaggggg tgaggcatca cccccg
//
LOCUS       DAZFFE010000255         1626 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFFE010000255 DAZFFE010000000
VERSION     DAZFFE010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.258"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            322..537
                     /gene="essD"
                     /locus_tag="ACHJ69_004918"
     CDS             322..537
                     /gene="essD"
                     /locus_tag="ACHJ69_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO5017696.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            537..734
                     /locus_tag="ACHJ69_004919"
                     /pseudo
     CDS             537..734
                     /locus_tag="ACHJ69_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
     gene            717..1626
                     /locus_tag="ACHJ69_004920"
                     /pseudo
     CDS             717..1626
                     /locus_tag="ACHJ69_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
BASE COUNT          401 a          372 c          458 g          395 t
ORIGIN      
        1 aacatcggaa attattcatt ggttatttgt tgaagttttc ttatgtattt gtggtggtgt
       61 tttgaacact cggtggcatt ctcacaaata tcatttagta gtttacgtac gtaaaaaatt
      121 ggttatgctg ttaagagtgg ttacttcgtc acacagctta aacccgccgt cgagcgggtt
      181 tttccatttt ttgagtctcg atattagctg ataacccaat acctgagtta ttcactgact
      241 ccgaatctgt tacgtttctg cctttattgc gatacgtagt atccccttaa tttacacccg
      301 ctttgtctgc gaggtggggt tatgaaatcc atggataagt taacaacggg tgtcgcctat
      361 ggcacctcag caggtagtgc cgggtactgg tttttacagt tgctcgataa agtcacgccc
      421 tcacagtggg cggcaatagg tgtgctgggt agtctggtat ttggcttgct gacgtatctg
      481 acaaaccttt atttcaagat taaagaagat aagcgtaagg ctgcgagagg tgaataatgt
      541 cgccatcatt acgcaaggct attgctgctg ctattggtgg tggggctgtt gccatagcgt
      601 ctgtgctcat cactggtccg agtggtgacg atggcctgga aggtgtcagc tacataccat
      661 acaaagatat tgttggtgta tggactgtat gtcacgggca tacaggaaaa gacatcatgc
      721 tcggtaaaac gtataaaggc agcagtaagg ggcatacccc gcgcgaagcg aaggacaacc
      781 tgaaatccac gcagttactg agtgtgatcg atgccatcag cgaagggccg attgaaggtc
      841 cggtggatgg gttaaaaagc gtgctactga acagtacgcc ggtgctggac actgagggga
      901 ataccaatat ctccggtgtc acggtggtgt tccgggcagg tgagcaggag cagactccgc
      961 cggaggggtt tgaatcctcc ggctccgaga cggtgctggg tacggaagtg aaatacgaca
     1021 cgccgatcac ccgcaccatc acgtcggcaa acatcgaccg tctgcgtttt accttcggtg
     1081 tgcaggcact ggtggaaacc acttcaaagg gtgaccggaa tccgtcggaa gttcgcctgc
     1141 tggttcagat acagcgtaac ggtggctggg tgacggaaaa agacatcacc attaagggca
     1201 aaaccacctc gcagtatctg gcctcggtgg tggtggataa cctgcctccg cgcccgttca
     1261 atatccggat gcgcaggatg acaccggaca gcaccacaga ccagctgcag aacaaaacgc
     1321 tctggtcgtc atacaccgaa atcatcgata tgaaacagtg ctacccgaac acggcactgg
     1381 tcggtgtgca ggtggactcg gagcagttcg gcagtcagca ggtgagtcgt aattatcatc
     1441 tgcgcgggcg cattctgcag gtgccgtaga actataaccc gcagacgcga caatacagca
     1501 gtatctggga cggaacgtta aaaccggcat acagcaacaa catggcctgg tgtctgtggg
     1561 atatgctgac ccatccgcgc tacggcatgg ggaaacgtct tggtgcggcg gatgtggaca
     1621 aatggg
//
LOCUS       DAZFFE010000256         1625 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFFE010000256 DAZFFE010000000
VERSION     DAZFFE010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.259"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..211)
                     /locus_tag="ACHJ69_004921"
     CDS             complement(<1..211)
                     /locus_tag="ACHJ69_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO5017697.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQRGGEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTD"
     gene            complement(204..572)
                     /gene="gpFI"
                     /locus_tag="ACHJ69_004922"
     CDS             complement(204..572)
                     /gene="gpFI"
                     /locus_tag="ACHJ69_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO5017698.1"
                     /translation="MATKEENQKRLRELAGLLGREADMSGSAADIAQRVAEWEEEVCA
                     SENEIADVDDTVYEQAYRNTGEDAFGILERIMLLKCFYLCGVDDETGEPVEHVDAGRV
                     ILMPPSVAKDMVKSGMAVYA"
     gene            complement(624..>1625)
                     /locus_tag="ACHJ69_004923"
     CDS             complement(624..>1625)
                     /locus_tag="ACHJ69_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO5017699.1"
                     /translation="LGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKITGKTPVAAY
                     VSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLNDPTYRRLRI
                     ITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKNNIIQGSGKE
                     WSKQDRDTFDPTYDIDLFCDQASGLVNIAIMDGTVWRLLNGFKLFREKLDTRRGSNSQ
                     LETAVKDLGSVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLGNTAAEGIRC
                     YGAIQDAQALSEGVVASSRYPKHWMTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK"
BASE COUNT          376 a          510 c          360 g          379 t
ORIGIN      
        1 tatccgtccg cacaaataat gacgggctgc ttccttcaat acggaccccg ccaccggcaa
       61 aacccagcga ctccggatcg tcaaaaaccc cctgaacttc gccaccacgc tgtgcccccg
      121 aggtgaactg cgcacagagt cccatcactt caacaatcgt gctgtccacc ccggcgaggg
      181 cagcatcgaa ggcattctga aaatcacgca taaacggcca ttccactctt gaccatgtct
      241 tttgccactg aggggggcat cagaattact ctgccagcat caacatgctc aacaggctca
      301 cctgtttcat cgtcaacacc gcacaggtaa aaacacttca gaagcatgat acgttccaga
      361 ataccgaaag catcctcacc ggtgttcctg tatgcctgct cataaacggt atcatcaaca
      421 tctgcgattt cattttccga tgcgcaaacc tcctcttccc actctgccac acgctgtgcg
      481 atatccgctg cactccccga catatccgcc tcgcgcccca gcaggccagc cagttcacga
      541 agacgtttct gattttcttc ttttgttgcc atctcggtct cctgtgaaaa aagacacggg
      601 ggcatttcgc ccccgctcac ggattatttc acctgtacca ccacaaactc atccggatcc
      661 ggcagcacca tcagcggagc ggactgcgtc atggtgaatt cacgggccgg atcgcccacg
      721 gtcatccagt gtttcggata acgggaagag gccaccacac cttcggacaa cgcctgcgca
      781 tcctgaatgg caccatagca acggatgccc tcagcagccg tatttcccag gaccagcgtg
      841 ccctccggca gataacgttt ttcggtaccg tcctctgcca cataagacgt tttcgccacc
      901 acaatggcca gatcaccgta ataccccttg aaagacacca ccgaccccag gtctttcact
      961 gccgtttcga gttgtgaatt tgagccgcga cgggtatcca gtttttcgcg gaacagctta
     1021 aagccattca gcagacgcca gacggtaccg tccatgatgg caatattcac aagaccgctg
     1081 gcctgatcac agaaaaggtc gatatcatat gtcgggtcga acgtgtcacg gtcctgtttt
     1141 gaccactcct taccgctacc ctgtatgatg ttattcttcg tcgatctgcc aaaatcaacc
     1201 tcaattttct cgaactggtc tccttccatg gtgtatttgc catacagcac ggcattcacc
     1261 gcctgcattt cttccacctg gacaatggcg tgctcttcct gtttgaggtt atcggtgatg
     1321 atacgcagac gacggtaggt cgggtcgttc agctgcgccg gatcttcacc gggaaggcgc
     1381 tccaccgcct gctggtaatt aaattcgtgt ttcggcttga cgtagcccgg acgtaacacg
     1441 cgggtttcac caccacgatg acgcagcact tttccttcaa caaccgggga gacataggct
     1501 gccaccggcg tttttccggt aattttgtcc agcatcacct cttcggtgtg gaaattcacc
     1561 gtacggcgga aaaacagttc cagaaacagc gcacgaaatt tcactttttg ttcggtataa
     1621 ccgag
//
LOCUS       DAZFFE010000257         1625 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFFE010000257 DAZFFE010000000
VERSION     DAZFFE010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.260"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..544)
                     /locus_tag="ACHJ69_004924"
     CDS             complement(<1..544)
                     /locus_tag="ACHJ69_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HFO5017700.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKQACCRCDHIVQAPVPS"
     gene            complement(564..911)
                     /gene="tnpB"
                     /locus_tag="ACHJ69_004925"
     CDS             complement(564..911)
                     /gene="tnpB"
                     /locus_tag="ACHJ69_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5017701.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            complement(911..1561)
                     /gene="tnpA"
                     /locus_tag="ACHJ69_004926"
     CDS             complement(911..1561)
                     /gene="tnpA"
                     /locus_tag="ACHJ69_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001690050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO5017702.1"
                     /translation="MNARKAALADNPELILRVLQLRFDESLSYPRISAQTGVSKTAIF
                     SLVRRFHQIFADWPLSGEYSCMQLARALFPGRYPSAPTVTQPVKTEKIRRNRFSPEFK
                     WRLVQQTLLPGACVAQIARENGINDNLLFNWRHLWRNGGLQPTGEHETSLLPVTLTPE
                     PDNKIPAPAQEPEQINTPPDSLCCELVLPAGTLRLKGELTPALLQTIIREMKGSSH"
BASE COUNT          354 a          425 c          453 g          393 t
ORIGIN      
        1 ttgaaggtac tggtgcctgc acgatatggt cgcaccggca acaggcctgt ttcggacgtt
       61 gtgtttcgat aaccttaaag gcgctgctga taagctccag ttgctctgac acatcacatc
      121 ccagagaact gagttcacca ccacaggcag gacagcattc ctcttccggc cggataaccc
      181 gggtttcacg gggaagtgag gccggtaacg gtttacgggc tgaagactgg cgcagggcgg
      241 atggcagtac cgggtcatat tgctcaccca gcgtttccgc catttcttcc tgaagtgcgc
      301 tgattcgctc ctgtgcttcc tgtatctgcc gttcggtttt tgcacgaagt ttttctgagc
      361 ttttaccgaa ctgcatacgt tgcagtttcg caaccagcgc cttcagccgg ttgatttcgg
      421 aagcataagc cgccacccgc tgtgagagca ggcggttgta ttcagccatc tggcggatgg
      481 tgtcctgttg cgtctgcaac agtgcccgca ggcgggcgtt ctcatgagca agtgaggtgt
      541 ccatatcctc actttacaac gggttatatg cggattccag cgcgttccgt tcgtttcggg
      601 tgcttccagt tgataccttc aagaagcata gataactgag ccggagtaag gtgcaccttg
      661 ccgtcacggg taaccggcca gacgaagcgg ccccgctcca ggcgtttggt gaagaggcac
      721 agtccgtcac tgtcagccca caacactttt atctggtcac cccggcgtcc gcggaagatg
      781 aacagatgcc cggagaacgg gtcatccttc aggacgttct gaacttttga tgccaggccg
      841 ttaaaaccat ttcgcatgtc ggtgataccg gcaaccagcc agatacgcga accggcaggg
      901 agagatatca tcagtggctg ctccctttca tttcgcggat aattgtctgt aataacgccg
      961 gtgtcagttc acctttcagc ctgagagttc cggctggcag aaccagctca cagcacagac
     1021 tgtcgggcgg tgtatttatc tgctcaggtt cctgtgcggg tgccgggatt ttattatccg
     1081 gctccggcgt taacgtcacg ggaagtagcg atgtttcatg ttcgccggtc ggctgcaggc
     1141 cgccgttacg ccagagatgc cgccagttaa agagcaggtt atcgttgatt ccgttctcac
     1201 gagctatttg tgcgacacag gctccgggta aaagagtttg ctgaacaagc ctccatttaa
     1261 actccggtga aaatcggttc cggcggattt tctctgtttt cacaggctga gtcactgtcg
     1321 gggctgaagg gtatcgccca gggaaaagag cccgggccag ttgcatgcag gaatattcac
     1381 cggaaagagg ccagtcagcg aatatctggt gaaatcgcct caccagagaa aaaatggcgg
     1441 ttttgctgac accggtctgc gcagaaatgc gcgggtacga cagtgactcg tcaaatctca
     1501 gttgtagcac acgcaggatc aattccggat tatctgccag tgccgccttt cgtgcattca
     1561 tcttaaatgt ccttttactg cgaaaatgga cattagtatc ggaaacagga aagggaggcg
     1621 aaaga
//
LOCUS       DAZFFE010000258         1509 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFFE010000258 DAZFFE010000000
VERSION     DAZFFE010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.261"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..80
                     /locus_tag="ACHJ69_004927"
     CDS             <1..80
                     /locus_tag="ACHJ69_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFO5017703.1"
                     /translation="FGLLTYLTNLYFKIREDRRKAARGE"
     gene            85..426
                     /locus_tag="ACHJ69_004928"
                     /pseudo
     CDS             85..426
                     /locus_tag="ACHJ69_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
     gene            complement(668..928)
                     /locus_tag="ACHJ69_004929"
     CDS             complement(668..928)
                     /locus_tag="ACHJ69_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017704.1"
                     /translation="MDYPNLPKQTFADLIALRQAVVALINVLPDEEKELVKAILNRTA
                     ADFSLFPLTDDLADLPELIAASAIKLTEEIYSPQKPSQNSCE"
     gene            947..1480
                     /locus_tag="ACHJ69_004930"
     CDS             947..1480
                     /locus_tag="ACHJ69_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5017705.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
BASE COUNT          420 a          302 c          417 g          370 t
ORIGIN      
        1 tgtttgggct gctgacatat ctgactaacc tgtatttcaa aatcagagag gaccggcgta
       61 aggcggcgcg gggagagtaa agtgatgaat aaaaaatacg aactggttgt taaagggata
      121 aataattacc cggataagat tactgttact gtggcactgg aaattggtgg gtatccgtca
      181 ctgttgttgc catatgtggc gattagtctt gaccgtactg aaggtgccac gctggaattt
      241 tacgaagctg aggcgaaaaa acaggcgaag cagtttttca tggatattgc tgccgcttta
      301 tgtgaagggg atgagcagtc gccggaaaag cgccccataa ttttagaggc gcaggatgtg
      361 ttgataacct acagaggaaa gctaccggga ataattactg gttctctgaa gacgccaccg
      421 acggcattgc ggtcagaaaa cgatgatatt gagtcacgca ttgaaaaact ggagtgctat
      481 atcgctgaat tgaaaaaaag caccccaaca aaaaatgagg tgcttgcagc agatgaaatg
      541 aaagaaacta ttcttgatcg cgcggcgcat ctaagctgcg cttcacggtt gaaagagcat
      601 cttcagcggc ctgaagaaaa acgccgcgat gagcagttcg cggcgtttta cgattattgc
      661 attgaagtta ctcgcaggaa ttttgtgaag gcttttgagg agagtaaatc tcttcagtaa
      721 gcttaatggc ggacgctgca attaattcag gaaggtccgc aaggtcatct gtcagtggga
      781 ataatgaaaa atcggcggca gttctgttaa gaattgcttt aactaattcc ttttcctcat
      841 ccggcaacac gttgattaga gctacgactg cttgcctgag tgcgattaaa tcagcaaaag
      901 tttgttttgg tagatttgga taatccataa atcctgataa atatccatga gcgcaaaaat
      961 caaatacggc ctgtcagctg ctgttctggc gctgattgct gcaggcgcgt ctgctcctca
     1021 gatacttgac cagtttctgg atgaaaagga aggcaaccac accacggcat accgtgatgg
     1081 tgcaggtatc tggaccatct gccgtggtgc catcatggtg gatggcaaac ctgtcgttcc
     1141 gggcatgaag ttgtcgaagg aaaaatgcga ccaggttaac gccattgagc gtgataaagc
     1201 gctggcgtgg gtggagaaaa atatcagagt gccgctgacc gaaccccaga aagcggggat
     1261 cgcgtcattc tgtccgtaca acattggtcc cggtaagtgt ttcccgtcga cgttttacag
     1321 acgaattaat gcaggtgatc gcaggggagc atgcgaagcg attcgctggt ggattaagga
     1381 cggtggcaga gactgccgta ttcgctcaaa caactgttac ggtcaggtat cccgtcgcga
     1441 ccaggagagt gcgctggcgt gctggggaat tgacagataa gcagaatatt ttgctgaaaa
     1501 atgcggttt
//
LOCUS       DAZFFE010000259         1439 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFFE010000259 DAZFFE010000000
VERSION     DAZFFE010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.262"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1085)
                     /locus_tag="ACHJ69_004931"
     CDS             complement(<1..1085)
                     /locus_tag="ACHJ69_004931"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017706.1"
                     /translation="MFLPLRFNKILSLFLLSSCSFNVMAFSDVDKGGQIRATGFQSEL
                     SGILTTRLLSLGADAATVAFVVKAYSQTAADFVVSYAARSGVAIGSMTWANLAYGLGV
                     TAASVGLYALGKPVAESFARMALSANGYDVTFATPAMTASDHNYWCGIISNGSGVYST
                     SPWSALARMKDAKYPGYSVSYSSLESRTATWYLALPGKPDDPGAVIQVGFNSGCPLDL
                     LNGSDDYPLCDNGEIAVQSEGATAYACEKATTEPVSFTGGTGGLTLDSSSVADVLNAL
                     SREAGQAGYVNGSGLTFTSEDVDSWRSHNDFSSDSTGFTDTSSGLGLPATSQGDTADS
                     GGTYSPPVAGAGAGAGTGTGTTTGTGTT"
     gene            complement(1255..>1439)
                     /locus_tag="ACHJ69_004932"
     CDS             complement(1255..>1439)
                     /locus_tag="ACHJ69_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017707.1"
                     /translation="GQVFAAGTTTTTGSVDFTPLTTSINFTSVVSVILSIGAAAVTVY
                     LAVIGVRAVWRQIKSI"
BASE COUNT          491 a          378 c          299 g          271 t
ORIGIN      
        1 gttgttcctg tgccggtggt tgtccctgtg ccagtgcctg cccctgcccc agcgcctgca
       61 acaggcgggc tgtaagtccc gccggaatca gcggtatctc cctgtgatgt tgcaggcaac
      121 cccaggccgg aagatgtatc cgtaaatcca gtagaatcag atgaaaaatc attatgcgaa
      181 cgccaggaat caacatcctc cgacgtaaat gtcagaccac tgccattaac atagccagcc
      241 tggccagcct cacgcgaaag tgcattcagt acatccgcaa cagacgaaga atccagcgta
      301 agtccgccag tcccgcccgt aaaactgacc ggctccgtgg ttgccttttc acaggcataa
      361 gctgttgccc cttcagactg aacagcaatt tcgccattat cacaaagagg ataatcatca
      421 ctaccattga gaagatccaa aggacagcca gaattaaatc ccacctgaat cactgctcca
      481 ggatcatccg gtttccctgg aagagccaga taccaggtag ccgttctgga ctcaagtgaa
      541 gaataagaaa cagaataacc gggatatttt gcatccttca tacgggcaag cgcagaccat
      601 ggtgacgtgc tgtaaacccc tgaaccatta ctgattatac cgcaccagta gttatggtca
      661 gatgccgtca tagctggcgt ggcaaacgtc acatcataac cattagccga taaagccatc
      721 cgcgcaaaag actccgcaac aggcttaccc agtgcataaa gccctactga tgcagccgtc
      781 acccccaaac cataagcaag gtttgcccag gtcatggagc caatggcaac accagaacgg
      841 gctgcataac tcacgacaaa atctgctgcc gtctgagaat acgctttaac gacaaaagca
      901 acagtggcag catcagcacc aagtgaaaga agccgtgttg tcagaatgcc ggataattca
      961 gactgaaacc ccgttgcacg tatctgacca cctttatcca catcagaaaa agccataaca
     1021 ttaaaagaac aggaagaaag aagaaaaaga gacaggattt tattaaaacg aagaggtaaa
     1081 aacataataa acatcctcaa ataataaaaa acattaatac ctcagcgact gaacataaat
     1141 ccgagcacag cagaaaaacc gcataatgca ccgaaaataa aaatcacgac atcaaaaata
     1201 gactgagaca tatatcacca taaaaaaggg gcaaaacatt gccccttcat aaaatcagat
     1261 agatttaatt tgacgccaga cagcacgaac cccgataact gcaagataaa cggtaacagc
     1321 agcagcaccg atagaaagaa ttacagagac aacagaagta aagttaatcg acgtagttaa
     1381 cggcgtaaaa tcaacagacc ccgtagttgt agtagtgcca gccgcaaaaa cctgaccgg
//
LOCUS       DAZFFE010000260         1419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZFFE010000260 DAZFFE010000000
VERSION     DAZFFE010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.263"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1397
                     /locus_tag="ACHJ69_004933"
     CDS             <1..1397
                     /locus_tag="ACHJ69_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5017708.1"
                     /translation="AERESSQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKI
                     LQADYNTLMAAAKKDYESTLKKPSAVKVSAGERQEDRAHAALLALETELRTLEKHSGV
                     NEKISQQRRDLWKAENQYVVLKEAATKRQLSEQEKSLLAHEKETLEYKRQLADLGDKV
                     EHQKRLNELAQQAARFEQQQSAKQAAISAKARGLTDRQAQRESEEQRLRDVYGDNPDA
                     LAKATSALKNTWSAEEQLRGSWMAGMKSGWGEWAESATDSFSQVKSAATQTFDGIAQN
                     MAAMLTGSEQNWRGFTRSVLSMLTEIFLKQAMVGIVGRIGSAIGGAFGGGASASTGTA
                     IQAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEG
                     GYVGAAGSPAQMRRAEGINFNQNNHVVIQNDGINGQAGPQLMKAVYEMARKGAQDELR
                     LQLRDGGLLSGSGR"
BASE COUNT          340 a          338 c          499 g          242 t
ORIGIN      
        1 gggctgagcg tgagtcgtca cagctgaagt ataccggaga ggcgcagaag gcgtatgagc
       61 gcctgctgac gccactggag aaatataccg cccggcagga agagctgaat aaggccctga
      121 aagacggaaa aatcctgcag gcggattaca acacgctgat ggcggcggcg aaaaaggatt
      181 atgagtcgac gctgaaaaag ccatctgctg tgaaggtgtc tgccggtgag cgccaggaag
      241 accgggcgca tgcagccctg ctggcgcttg aaaccgagct caggacgctg gaaaaacaca
      301 gcggtgtgaa tgagaaaatc agccagcagc gccgggattt atggaaagcg gaaaatcagt
      361 atgtggtcct gaaagaggcc gccacgaaac ggcagttatc tgagcaggaa aaatccctgc
      421 tggcccatga gaaagagacg ctggagtaca aacgccagct ggctgacctg ggcgacaagg
      481 ttgaacacca gaaacggctg aatgagctgg cacagcaggc ggcgcggttt gaacagcagc
      541 agagcgcgaa gcaggccgcc atcagcgcaa aagcccgcgg cctcaccgac cgtcaggcgc
      601 agcgggagtc ggaagagcag cgccttcgtg acgtgtacgg tgataatccg gatgcgctgg
      661 cgaaggccac atctgcactg aagaacacct ggtctgcgga ggagcagctt cgtggaagct
      721 ggatggccgg gatgaagtcc ggctggggag agtgggcgga aagtgcgacg gacagttttt
      781 cgcaggttaa aagcgcggcc acgcagacct ttgacggtat tgcacagaat atggcagcga
      841 tgctgaccgg cagcgaacag aactggcgtg gtttcacccg ttccgtgctc tccatgctga
      901 cagagatttt tctgaagcag gcgatggtgg ggattgtcgg gcgtatcggc agcgccattg
      961 gtggtgcttt cggtggtggc gcatccgctt ccacggggac ggccattcag gctgcggcgg
     1021 cgaacttcca tttcgcgacc gggggattta cgggaaccgg tggcaaatat gagccagcgg
     1081 ggattgtcca ccgcggggag tttgtcttca cgaaggaggc aaccagccgg attggtgtcg
     1141 ggaacctgta ccgcctgatg cggggctatg cggaaggggg gtatgtgggt gctgccggaa
     1201 gtccggcgca gatgcggcgg gcggaaggta ttaattttaa tcagaacaat cacgtggtga
     1261 ttcagaacga cggcatcaac ggacaggccg ggccgcagct gatgaaagcg gtgtatgaga
     1321 tggcccgtaa aggtgcgcag gatgagctcc ggctgcagtt gcgtgatggc ggtctgttat
     1381 cggggagcgg gcgatgaaaa cctttcgctg gaaagtgaa
//
LOCUS       DAZFFE010000261         1413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZFFE010000261 DAZFFE010000000
VERSION     DAZFFE010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.264"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1413
                     /locus_tag="ACHJ69_004934"
     rRNA            <1..>1413
                     /locus_tag="ACHJ69_004934"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          352 a          313 c          445 g          303 t
ORIGIN      
        1 cgtagtcgat gggaaacagg ttaatattcc tgtacttggt gttactgcga aggggggacg
       61 gagaaggcta tgttggccgg gcgacggttg tcccggttta agcgtgtagg ctggttttcc
      121 aggcaaatcc ggaaaaccaa ggctgaggcg tgatgacgag gcactacggt gctgaagcga
      181 caaatgccct gcttccagga aaagcctcta agcatcaggt aacatcaaat cgtaccccaa
      241 accgacacag gtggtcaggt agagaatacc aaggcgcttg agagaactcg ggtgaaggaa
      301 ctaggcaaaa tggtgccgta acttcgggag aaggcacgct gatatgtagg tgaagcgact
      361 tgctcgtgga gctgaaatca gtcgaagata ccagctggct gcaactgttt attaaaaaca
      421 cagcactgtg caaacacgaa agtggacgta tacggtgtga cgcctgcccg gtgccggaag
      481 gttaattgat ggggttagcg gcaacgcgaa gctcttgatc gaagccccgg taaacggcgg
      541 ccgtaactat aacggtccta aggtagcgaa attccttgtc gggtaagttc cgacctgcac
      601 gaatggcgta atgatggcca ggctgtctcc acccgagact cagtgaaatt gaactcgctg
      661 tgaagatgca gtgtacccgc ggcaagacgg aaagaccccg tgaaccttta ctatagcttg
      721 acactgaaca ttgagccttg atgtgtagga taggtgggag gctttgaagt gtggacgcca
      781 gtctgcatgg agccgacctt gaaataccac cctttaatgt ttgatgttct aacgttggcc
      841 cctaatcggg gttgcggaca gtgtctggtg ggtagtttga ctggggcggt ctcctcctaa
      901 agagtaacgg aggagcacga aggttggcta atcctggtcg gacatcagga ggttagtgca
      961 atggcataag ccagcttgac tgcgagcgtg acggcgcgag caggtgcgaa agcaggtcat
     1021 agtgatccgg tggttctgaa tggaagggcc atcgctcaac ggataaaagg tactccgggg
     1081 ataacaggct gataccgccc aagagttcat atcgacggcg gtgtttggca cctcgatgtc
     1141 ggctcatcac atcctggggc tgaagtaggt cccaagggta tggctgttcg ccatttaaag
     1201 tggtacgcga gctgggttta gaacgtcgtg agacagttcg gtccctatct gccgtgggcg
     1261 ctggagaact gaggggggct gctcctagta cgagaggacc ggagtggacg catcactggt
     1321 gttcgggttg tcatgccaat ggcactgccc ggtagctaaa tgcggaagag ataagtgctg
     1381 aaagcatcta agcacgaaac ttgccccgag atg
//
LOCUS       DAZFFE010000262         1408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFFE010000262 DAZFFE010000000
VERSION     DAZFFE010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.265"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            47..471
                     /locus_tag="ACHJ69_004935"
                     /pseudo
     CDS             47..471
                     /locus_tag="ACHJ69_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1008..1280
                     /locus_tag="ACHJ69_004936"
     CDS             1008..1280
                     /locus_tag="ACHJ69_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin biosynthesis transcription regulatory
                     family protein"
                     /protein_id="HFO5017709.1"
                     /translation="MTPSPYPRNNFNTELIQSCMNGEHFSLLIEISPIRSKKNIMALK
                     EYLVDGYSKQESCERNNVSISYFCLCLKKLNYTHSIVSALKYFYRE"
BASE COUNT          433 a          281 c          292 g          402 t
ORIGIN      
        1 agtgatagtc ttaatactag tttttagact agtcattgga gaacagatga ttgatgtctt
       61 aggaccggag aaacgcagac ggcgtactac acaggaaaag atcgctattg ttcagcagag
      121 ctttgaaccg ggaatgacgg tctcccttgt tgcccggcaa cacggtgtgg cagccagcca
      181 gttatttctc tggcgcaagc aataccagga gggaagtctt actgctgtgg ccgcaggaga
      241 acaggtcgtt cctgcctctg aacttgctgt tgccatgaag cagattaaag aactccagcg
      301 cctgctcggc aaaaaaacga tggaaaatga actccttaaa gaagccgttg aatatgggcg
      361 tgcaaaaaag tggatagcgc acgcgccctt attgcccggg gatggggagt aagcttagtc
      421 agccgttgtc tccgggtgtc gcgtgcgcag ttgcacgtca ttctcagacg aaccgatgac
      481 tggaaatata ggggcaaatc cacatcctta cttttccgta aggattttag cgggtcaaaa
      541 cagcgttcca tgccggactg atgatctaca tacttagagt cacataccaa agtaaaacta
      601 tggatgtatg caagcctgca tcgtcaggag gttaaatttc agacctgcca gttaccgcgt
      661 aaatggcttg atatatgact aatttatttg cacaggattt ttacgacaac gctctgaacc
      721 agcagttgac gggatatata gttgagctca ttacagataa agcttttctg tatctctggc
      781 ggtggtcata gaccagtaat cgttattggt tgttctatat tatcccgtat gacggtacaa
      841 cgtttttaac aactggcgct gtcacaacat aagccacccc aaaatattat atgagtaaca
      901 atagtctata taggagcgta aattacctac tatcaaatac ttactccaat agcatttctg
      961 tctattctat ttcaattctt taatagaaaa ataaggttag ataatatatg actccctccc
     1021 cctatccaag aaataacttt aatacagagc taattcaatc atgtatgaat ggagaacact
     1081 tttcactttt aattgaaata tcaccaatca gaagtaaaaa aaacatcatg gctcttaagg
     1141 agtatttggt tgatggttat tcaaagcaag aaagttgtga aaggaacaat gtttccatta
     1201 gttatttttg cctatgccta aaaaaactaa actatacaca cagtattgtt agtgctctta
     1261 aatatttcta ccgtgagtaa actgtaactg aagtcttgat tcttacgctt agacactgct
     1321 aagccgttat ggcatagctg ataatttata ttgcactatg tcagctactg taagataata
     1381 taagctattt taaagaaaac tctgtcat
//
LOCUS       DAZFFE010000263         1394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFFE010000263 DAZFFE010000000
VERSION     DAZFFE010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.266"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(302..406)
                     /locus_tag="ACHJ69_004937"
     CDS             complement(302..406)
                     /locus_tag="ACHJ69_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017710.1"
                     /translation="MSDDISLAMEGALAVIAVVGVYCLVVFLMDRLGN"
     gene            complement(522..761)
                     /locus_tag="ACHJ69_004938"
                     /pseudo
     CDS             complement(522..761)
                     /locus_tag="ACHJ69_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
BASE COUNT          329 a          393 c          298 g          374 t
ORIGIN      
        1 atgacggtaa tacagatgac gatcagggcg actaacatcg ccttttgctg cttcatagcc
       61 tgcttctcct tgcctttcgg cgtgtaagag gctaacctac atgtgactag catgaaattg
      121 gcctcagatt aatgttaagc gtcctgcaag acgcataatg ttaactgggg cttttctctg
      181 tctgccttac ggtggcatgc ccgaggcaga cagcctcaag cacccgcagc aattctactt
      241 aactctcgct ttaccgcaaa ccgtttttac ccgatatggg aattcccata tcgtaatgaa
      301 ttcagttccc tagtcgatcc atcaaaaaca caaccaggca gtaaacaccc acaacagcaa
      361 taacagccag cgcaccttcc attgccagtg agatatcatc cgacacattc cctcctttgg
      421 tgttaatccc ggcgaacgct tttacccaca ccgacaaata acatatacta aaaaagcgat
      481 agccatagca acgcctgtaa ttgcaaatgc ttcaggctag gtcattggcg tacctccttc
      541 ggcggttctg gtagcggcat ccagtgggtt actttcgatg ccggttcttc cccattgtca
      601 gtaactgccc accatttgtt tctcgaccaa tcgtaatacc cttcgaaggt atcgcactca
      661 gtccagccgt aagacttacc ccaacaccaa acatactgtt tatcgttcgg cattcgctca
      721 ctacagctta tccaaccatc cggagttacc ggagagttgc ccgatagtgc attctgctcc
      781 agtgatgctt ttacaaacca cgctgcctga actataacgc catgaatcca gcgcaaatca
      841 gcatcgcgat ctttcttttt catcttttcg ccacttaagg ccttgcttat gtggctgcgt
      901 accaggtctt catgcaattc cttcgcctcc tcaatggtga aaccaccagg cagaagagcc
      961 ggagttaccg gagagctggt tgacgcttcc gggatttccc gaaaattatt ggttgacgaa
     1021 ttcttgcttt cccgaaagtt tccggactga agcatggcgg tgcggcaggc gttccatatt
     1081 tcggcagcaa tatcgcgctc gctatcggtt aatttgtacg tggaaacata gccagagagc
     1141 atttctacgt tttccggagt tgcttcttcc ggcactaccg gcgctggcgg ggcggcaaat
     1201 agatatccgc caaagtcagg aagctctcca atggcctgta cgaacttttg tttgcctacg
     1261 tcaactccta atgggtaatg agctataatc tttgccaccg gctctgcttc cagcgatacc
     1321 agtgcaattc gtgccagttc ttccgcttct tctgctggca gtacaacgtt gctacccggt
     1381 ccgtatgttt cgcg
//
LOCUS       DAZFFE010000264         1390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFFE010000264 DAZFFE010000000
VERSION     DAZFFE010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.267"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1390
                     /locus_tag="ACHJ69_004939"
     CDS             <1..>1390
                     /locus_tag="ACHJ69_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFO5017711.1"
                     /translation="TRAVINKLKDQKLLAPAPAQLIRLAMAGRAAARRLSYRELIRDL
                     KPSSISALEQLLTERVGEKSILGWIAEAPEGVKLKNLKGLIARLEVLRTAAISDEQRK
                     RIHVNRYGIIARDSRVLHAREIQRMASERRHATLVAFVIEKQAAITDLAIDMFCKLIG
                     STRRRAEISQKERRLKAAEVLEVVAQDHIKLGRALLAARDGNTDIESVITASLGWEGL
                     IASMEAAASVASSHHCSELDELIGRQKSLRKLGRLIFSAFSFRAFRPDDSVLLSIGYL
                     QAFYHGRKLPKQIPLDFMSHKWRRRVIHGENTDLQAWEVAVFVHLRERLKTGDIWVDG
                     SRAWRSFEDYLLPHHVFGQMRAESRLGLAIPDNFAEWRAERTTTLNAKLKALAKAASE
                     NAISDAELSSRGLSVSPIREEESDKIKALSRKLYMLVPRIRITSLLAEVHSWTSFLDC
                     FTHYRTGEQAED"
BASE COUNT          355 a          326 c          367 g          342 t
ORIGIN      
        1 tcaccagagc agttatcaat aagctgaagg accagaagtt acttgcccct gctccggcac
       61 aactcatccg tctggcaatg gcgggccggg cggctgcccg tcgcctttct tatcgggaac
      121 tcatcaggga tctgaaacct tcatccatta gcgctctcga gcagttgctt accgaacgag
      181 tcggagaaaa gagcatttta ggctggatag ctgaagcgcc ggaaggcgtg aaactcaaga
      241 atcttaaggg gttaattgcc aggctggagg tattacgaac agcggcaatc tccgatgagc
      301 aacgtaaacg gattcatgta aaccgttacg gtattattgc ccgggattct cgcgttttac
      361 atgcgcgtga aatacaacga atggcatccg aacgccgtca tgccacgctg gtagcctttg
      421 ttattgaaaa gcaggcagca attacagatc tggcaattga tatgttctgc aaattgattg
      481 gcagcacgag gcgcagagca gagataagcc agaaagagcg tcgtctgaaa gccgctgaag
      541 tacttgaagt cgtagctcag gatcatatta agctgggacg ggctctcctg gcagcacgtg
      601 acggtaatac tgatattgaa tctgtaataa cagcatcact cggctgggaa ggtttgattg
      661 ccagtatgga ggctgcagct tcggtggcat cttcccatca ctgttctgaa ctggatgaac
      721 ttatcggacg acaaaaatca ttacgtaagc ttggacgact cattttcagc gctttttctt
      781 tcagagcttt tcgcccggat gatagcgttt tgttgtcgat tgggtatttg caggcgttct
      841 atcatggccg aaaattaccc aaacaaattc cgctcgattt tatgtcccat aaatggcgac
      901 gtcgtgtcat ccatggagaa aataccgatc tgcaggcgtg ggaagtcgcc gtattcgttc
      961 atttgcggga acgcctgaaa acaggtgaca tatgggttga tggtagtcgg gcctggcgta
     1021 gttttgagga ctaccttctt cctcaccatg ttttcgggca gatgcgggca gaatcccggc
     1081 tgggacttgc cattccggac aactttgctg aatggcgggc tgagcgaacc accacactca
     1141 acgcaaaact gaaagcgctg gcaaaagctg cttcggagaa tgccatatcc gatgcagaac
     1201 tttccagcag agggttgtct gtgtctccga ttcgcgaaga agagagcgac aaaattaagg
     1261 ctctcagccg taaactctac atgctggtac cacgcatcag aattaccagt ttgctggcag
     1321 aagttcatag ctggaccagt tttcttgatt gtttcaccca ttatcgcacc ggggaacagg
     1381 cggaagatga
//
LOCUS       DAZFFE010000265         1381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFFE010000265 DAZFFE010000000
VERSION     DAZFFE010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.268"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..551
                     /locus_tag="ACHJ69_004940"
     CDS             <1..551
                     /locus_tag="ACHJ69_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017712.1"
                     /translation="RPAEDNFMEKVDNLNSEQQYDNIAGTTNLGYQKQFGEARRELAG
                     NLAQSGVDPSSGRFNAVMDANQSDQVIGQIDMTTRGQVSQADKYIAGLQDVAALGSGQ
                     KADALQSFNSLADSSLAKAKSDAQAAFTKQQGRASLVGAGLGAVGAYAMHKAGGNGGG
                     GGAKTPGTGANAIQHQAQNWRL"
     gene            554..1000
                     /locus_tag="ACHJ69_004941"
     CDS             554..1000
                     /locus_tag="ACHJ69_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017713.1"
                     /translation="MEYGKYETLARAGYSGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVNRPDNPNSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQQLSVGGQLVSA"
     gene            1010..1261
                     /locus_tag="ACHJ69_004942"
     CDS             1010..1261
                     /locus_tag="ACHJ69_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFO5017714.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRREEIEAANKQMAAQQKAQ
                     NKQNIGTGIGTGAAMGAAVGGLPGAAIGAVIGGLAGSLF"
     gene            1272..>1381
                     /locus_tag="ACHJ69_004943"
     CDS             1272..>1381
                     /locus_tag="ACHJ69_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017715.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQ"
BASE COUNT          355 a          317 c          431 g          278 t
ORIGIN      
        1 tccgcccggc tgaagataac ttcatggaaa aggtcgataa ccttaacagt gagcagcagt
       61 acgacaatat tgctggcacc acaaatctgg ggtatcagaa acagtttggt gaagcgcgaa
      121 gggagctggc gggtaatctt gctcagtccg gcgttgaccc atccagtggt cgttttaatg
      181 cagtaatgga tgcaaatcaa agcgaccagg tgatcgggca gattgacatg accacacggg
      241 ggcaggtatc gcaggcagat aaatatattg ccgggctaca ggatgttgcg gctctcggtt
      301 ccggtcagaa ggcagatgcg ttacagagtt ttaactcctt ggctgacagc agtctggcaa
      361 aagccaaatc ggatgcacag gcggcgttta cgaaacaaca ggggcgagct tctcttgttg
      421 gcgctggtct gggagctgta ggtgcgtacg cgatgcataa ggctggcggt aacggaggtg
      481 gtggcggcgc taaaacgcca ggcaccggcg ctaatgctat tcagcatcag gctcagaact
      541 ggagactgta attatggagt atggcaaata cgaaactctc gcaagggctg gttattcagg
      601 cgcagcccgc ccacagggtg actggcagac atccgccgcg ctgacacgcc agcaatacga
      661 cgactggcga accagatatt tgccccgcgt ggcaaggctg gctgaccttg gtgagaacaa
      721 cagcctgatg aatgcacagc ttgcccgggt aggtggcctt gccacttcca gtctccgtac
      781 agcgcagatg gcacaggata accagatggc gagatacggg gtaaaccgtc cggataatcc
      841 caacagtaac acgctgggtt tacgtaatgc actggcaatt gctggcgcga aaaatggtat
      901 acgggaagca gagcaggatc gccagatgaa catactgacg ggggcttctg cacctgcaag
      961 acagcaactg agtgttggcg gacaactggt gtcagcgtaa ggaggaaata tgggatacgg
     1021 tttactggat attgcgaatc agtcgcggcg tgaggcatta cagggaataa gtgatgccga
     1081 cagacgacgt gaagaaattg aggccgcgaa caaacagatg gcggcgcaac agaaagcgca
     1141 gaacaaacag aatatcggca cgggaattgg tacgggtgca gctatgggcg ctgctgttgg
     1201 tggtctcccc ggtgccgcta ttggtgcagt catcggtggt cttgctggtt cgttgtttta
     1261 aggagtggtg aatgagcgga tttgcacagg ggttacttgc cggattcagc accgttgacc
     1321 aggccatgac ccgtcgtaag gaacttggtt tgcgagaagc acagcttgct caacaacaga
     1381 a
//
LOCUS       DAZFFE010000266         1352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZFFE010000266 DAZFFE010000000
VERSION     DAZFFE010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.269"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..380
                     /locus_tag="ACHJ69_004944"
     CDS             <1..380
                     /locus_tag="ACHJ69_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO5017716.1"
                     /translation="ITYKVQRDKKARELELANATITDMQVRQRDVAALDAKYSRELAD
                     ARAENETLRADVAAGRKRLRINATCSGTVREATGTSGMDNATGPRLADTAERDYFTLR
                     ERLMMMQKQLEGAQEYIRTQCLK"
     gene            682..>1352
                     /locus_tag="ACHJ69_004945"
     CDS             682..>1352
                     /locus_tag="ACHJ69_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO5017717.1"
                     /translation="MAKLDWKKLEQAFRREHARTGIKLQDWCRQNNICYGTARRYIKL
                     RKNSPKNTEESAQKNAQKSAQIDTQKSAQKNAHGADDESSNDESYGDDGSDEKCAKNC
                     ANSAETKRNSRSGNPHPVARFSDRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVF
                     TRARALSVTGTLKKMFADLKEAADVETRVALYDKILKAEQALDRNIARIESIERSLLT
                     LDVLA"
BASE COUNT          396 a          337 c          357 g          262 t
ORIGIN      
        1 ccatcaccta caaagtgcag cgcgataaaa aagccagaga gctggagctg gcaaacgcca
       61 ccattactga tatgcaggtg cgccagcgcg atgttgctgc gctcgatgca aaatactcga
      121 gggaattagc cgatgcgaga gctgaaaatg aaactctgcg tgctgatgtt gctgctggtc
      181 gtaagcgcct gcggatcaac gccacctgct ccggtaccgt gcgtgaagcc accggcacct
      241 ccggcatgga taatgcaacc ggcccccgac tggcagacac cgctgaacgg gattatttca
      301 ccctcagaga gcggttgatg atgatgcaga agcagctgga aggggcacag gaatatatcc
      361 gcactcagtg cctgaaataa gttttgctga tgcgccgtat cgtcgcagta ttcccgcatt
      421 aacagagacc tcagccagac actggacctc ctgcgcgagt gtgtggggat aatcaaaaac
      481 gatgcacacc gggctttcca cgttttcagg taacgtgagc gttttcccgc atggccgcct
      541 gtccggtgcg gtggtggaag aaaccggata aaacaaccat attgtgtaaa tatcaatcaa
      601 acaagacgct gttgtgtgaa ttctgaaaaa tcacagcggt cattatgcgt cagtttttaa
      661 cagaggacgt cagaaagtga catggcaaag ctggactgga aaaaactgga gcaggcattc
      721 cgacgcgaac atgccagaac tggcataaaa cttcaggact ggtgtcgaca gaacaatatc
      781 tgctatggca cggctcgtcg gtacataaaa ctgcgcaaaa actcccccaa aaatacagag
      841 gaaagtgcgc agaaaaatgc gcaaaaaagt gcgcaaattg acactcaaaa aagcgcacaa
      901 aaaaatgcgc atggagctga tgacgagtcc agtaatgacg agtcttacgg cgatgatggc
      961 agtgatgaaa aatgcgcaaa aaactgcgca aattcggcag aaacgaaacg caacagcaga
     1021 tcgggcaatc ctcaccccgt tgcccggttt agcgaccgta acacccacgc cgtgagacac
     1081 cgtggatatg cgaagtatct tgaggcagat aacctcatgg atgatgcgtc cgatatggtg
     1141 ctgttcgatg aactggtgtt cacccgtgcc cgcgcacttt cagtaacagg aacactgaaa
     1201 aaaatgttcg ccgacctgaa agaggcggct gacgtggaaa cccgtgttgc cctgtacgac
     1261 aaaatactca aagcagaaca ggcccttgac cggaatattg cccgtatcga gtcaattgaa
     1321 cgctcattgc tgacgctgga cgttctggcg ga
//
LOCUS       DAZFFE010000267         1326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZFFE010000267 DAZFFE010000000
VERSION     DAZFFE010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.270"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            548..>1326
                     /locus_tag="ACHJ69_004946"
     CDS             548..>1326
                     /locus_tag="ACHJ69_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017718.1"
                     /translation="MNKKFSKTILSSAVAGLLLVSGGAMAEDIKIGDNYIIRISENNE
                     SARLLDKSGKDALLGLINTKTGAITTVNKTELEQKIKNTRGVEPQRIMESIRYTIDAP
                     SLKKENINKLTSADLDKIKANVEVVSKTITSETAPVYREAIQNGMTSEDALAVVNQDK
                     TGGLLKQFNGLNTNVNALKNATTFAIDENGEITDGQGVETVSVKSVVADVKADTTIYQ
                     NKDGSYTLDQSAPGNVRVNDAVVSLDNRTRSNTQAIQNHSR"
BASE COUNT          438 a          240 c          308 g          340 t
ORIGIN      
        1 agaaggcact ggaagcgctg aacagtgcgg atgagattct ggcgtataag gtgggtcagg
       61 agtcgtaaat aaaaaaacgg gggtgatgcc tcaccccctc tttcgtcaat agaggattac
      121 agcaatcctt tttttattgt gaaacagatt ctgcaataac cgccaggcat attcagtgat
      181 gcctgtcaca cgctccggaa cgcggatggc agcggcatgt cagaaaacga ttaatattat
      241 taatgcatcg aaaaaaatca gaatgtggtg cttatcacat tattaagcgt aaggaactgt
      301 gtcataagtg cgtgagggaa ataagcgaaa cgacagcatg cggtgtggag cgtgaataat
      361 ggcggggatg acggatatca ccgactgtac agtacgataa ttcctaaaaa gttcatattc
      421 atttttgcga ataatcctgg aataagctga caggaagcgg ccagtcatca gattttcttt
      481 agtgtatctg aagtgcgtct gagagggttg ctttttttgt atacaaagaa attacaggat
      541 atatcagatg aacaaaaaat tctctaagac tattctttct tctgctgttg ccggactgtt
      601 gctggtgagt ggtggggcta tggcagagga tattaaaatt ggtgacaatt atattattcg
      661 catttcagaa aataatgaaa gtgcacgtct gcttgacaaa agtgggaaag atgctcttct
      721 gggacttatt aatacaaaaa caggtgctat tactactgtt aataaaacag agctcgaaca
      781 aaaaataaag aatactagag gcgttgagcc gcaaagaatt atggagtcta ttcgttatac
      841 aattgatgcg ccatctttaa aaaaagaaaa tattaacaaa ttaacatctg ccgatcttga
      901 taaaataaag gctaatgtag aggttgtttc aaaaaccatc acatcagaaa ccgcacctgt
      961 ttatcgcgaa gctattcaaa acggcatgac atcagaagat gcgttggcag tggtaaacca
     1021 ggataagacg ggtggattac tgaagcaatt caacggtctt aatacaaatg taaatgctct
     1081 gaaaaatgcc accacgtttg ccattgatga aaatggtgaa attactgatg gacagggcgt
     1141 tgaaactgta tcagtgaaga gcgttgttgc agatgtgaaa gccgatacaa caatttatca
     1201 gaataaagat ggcagctata cacttgacca gagcgcacct ggcaacgttc gtgtgaatga
     1261 tgctgttgtc agcctcgaca acagaacccg cagtaacacc caggctatcc agaatcactc
     1321 ccgcca
//
LOCUS       DAZFFE010000268         1313 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFFE010000268 DAZFFE010000000
VERSION     DAZFFE010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.271"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1058
                     /gene="hyxB"
                     /locus_tag="ACHJ69_004947"
     CDS             <1..1058
                     /gene="hyxB"
                     /locus_tag="ACHJ69_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane autotransporter HyxB"
                     /protein_id="HFO5017719.1"
                     /translation="DLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADGNFYFV
                     KNTHKASNASSVIQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWIQYFGGKQK
                     HTTAGNASYDLDVNGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSKGDTEGYSF
                     HAYLSRQYNNGIFIDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAIVKGGYTWK
                     DGNGLFIQPYAKLSALTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDFAVGNATVK
                     PYLNLAALNEFSDGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTKG
                     DDIENPLQGVVGINVTW"
     gene            1242..>1313
                     /locus_tag="ACHJ69_004948"
     CDS             1242..>1313
                     /locus_tag="ACHJ69_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017720.1"
                     /translation="MNEQIKQDIDLIEILFYLKKKIRV"
BASE COUNT          353 a          293 c          330 g          337 t
ORIGIN      
        1 tggatcttat caatgaacaa acggcattta tttctaccgg caaagacagc actctaaaag
       61 ccacaggcac aacggaaggt ggtctgtatc aatatgacct gacacaggga gctgatggta
      121 acttttattt cgtaaaaaac acgcataaag catccaacgc cagctccgtg attcaggcaa
      181 tggcagctgc tccggctaac gtcgctaatc tgcaggctga cacgctctcc gcccgtcagg
      241 atgctgtccg tctgagcgaa aatgacaagg gtggcgtatg gattcagtac tttggcggta
      301 aacagaaaca taccaccgcg ggaaatgcat cctatgacct ggatgtaaat ggtgtaatgc
      361 tgggtggtga tacccgcttc atgactgaag atggtagctg gctggccggt gtggcgatgt
      421 cttctgcgaa aggtgacatg actaccatgc agagcaaagg tgacactgaa ggttacagct
      481 tccacgctta cctgagccgc cagtataaca acggtatctt cattgatact gctgcacagt
      541 ttggtcacta cagcaacacg gcagatgttc gcctgatgaa tggtggcggt accatcaaag
      601 ctgactttaa caccaatggt tttggtgcga tagttaaagg cggttacaca tggaaagacg
      661 gtaatggcct gtttattcag ccatatgcca aactgtctgc tctgactctg gaaggtgtgg
      721 attatcaact caacggcgtg gacgttcatt ctgacagcta taactctgtg ctgggtgagg
      781 ccggtacgcg cgtgggttat gacttcgctg tgggcaacgc gaccgttaaa ccttatctga
      841 atctggccgc actgaacgaa ttctctgatg gcaacaaagt ccgtctgggt gatgagtctg
      901 tcaatgccag cattgacggt gcagcattcc gcgtgggtgc aggtgtacag gctgatatca
      961 ccaaaaacat gggagcatat gcaagccttg actacaccaa aggtgacgac attgagaacc
     1021 cgctacaggg tgtagttggt atcaatgtga cctggtaaga ataaagcagt cggttcccct
     1081 gtcgactgtt ataccccctg caggcagcag tatgctgcct gcaggctata aatatccagt
     1141 attcatgatg ctggctttgt gctgatattt accctgaaca ttcctgatac agactattaa
     1201 tacaatctta tctgcgacag atttcatttt ctggatatat tatgaatgaa caaatcaaac
     1261 aggatatcga tctgattgag attttatttt atctgaagaa aaagattcgt gtt
//
LOCUS       DAZFFE010000269         1310 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZFFE010000269 DAZFFE010000000
VERSION     DAZFFE010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.272"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1310
                     /locus_tag="ACHJ69_004949"
     rRNA            <1..1310
                     /locus_tag="ACHJ69_004949"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          326 a          304 c          414 g          266 t
ORIGIN      
        1 gcccagatgg gattagctag taggtggggt aaaggctcac ctaggcgacg atccctagct
       61 ggtctgagag gatgaccagc cacactggaa ctgagacacg gtccagactc ctacgggagg
      121 cagcagtggg gaatattgca caatgggcgc aagcctgatg cagccatgcc gcgtgtatga
      181 agaaggcctt cgggttgtaa agtactttca gcggggagga agggagtaaa gttaatacct
      241 ttgctcattg acgttacccg cagaagaagc accggctaac tccgtgccag cagccgcggt
      301 aatacggagg gtgcaagcgt taatcggaat tactgggcgt aaagcgcacg caggcggttt
      361 gttaagtcag atgtgaaatc cccgggctca acctgggaac tgcatctgat actggcaagc
      421 ttgagtctcg tagagggggg tagaattcca ggtgtagcgg tgaaatgcgt agagatctgg
      481 aggaataccg gtggcgaagg cggccccctg gacgaagact gacgctcagg tgcgaaagcg
      541 tggggagcaa acaggattag ataccctggt agtccacgcc gtaaacgatg tcgacttgga
      601 ggttgtgccc ttgaggcgtg gcttccggag ctaacgcgtt aagtcgaccg cctggggagt
      661 acggccgcaa ggttaaaact caaatgaatt gacgggggcc cgcacaagcg gtggagcatg
      721 tggtttaatt cgatgcaacg cgaagaacct tacctggtct tgacatccac ggaagttttc
      781 agagatgaga atgtgccttc gggaaccgtg agacaggtgc tgcatggctg tcgtcagctc
      841 gtgttgtgaa atgttgggtt aagtcccgca acgagcgcaa cccttatcct ttgttgccag
      901 cggtccggcc gggaactcaa aggagactgc cagtgataaa ctggaggaag gtggggatga
      961 cgtcaagtca tcatggccct tacgaccagg gctacacacg tgctacaatg gcgcatacaa
     1021 agagaagcga cctcgcgaga gcaagcggac ctcataaagt gcgtcgtagt ccggattgga
     1081 gtctgcaact cgactccatg aagtcggaat cgctagtaat cgtggatcag aatgccacgg
     1141 tgaatacgtt cccgggcctt gtacacaccg cccgtcacac catgggagtg ggttgcaaaa
     1201 gaagtaggta gcttaacctt cgggagggcg cttaccactt tgtgattcat gactggggtg
     1261 aagtcgtaac aaggtaaccg taggggaacc tgcggttgga tcacctcctt
//
LOCUS       DAZFFE010000270         1297 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZFFE010000270 DAZFFE010000000
VERSION     DAZFFE010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.273"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..488)
                     /locus_tag="ACHJ69_004950"
     CDS             complement(<1..488)
                     /locus_tag="ACHJ69_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5017721.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPVEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETVLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPTSVRLLIQLERGGKWMTEKDVTINGKTT
                     SQ"
     gene            complement(743..>1297)
                     /locus_tag="ACHJ69_004951"
     CDS             complement(743..>1297)
                     /locus_tag="ACHJ69_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5017722.1"
                     /translation="ALSLQVPGFRRQMNEGWYQIRIAGDDTAPEAVYARLHEPLGEGT
                     VIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGATMALWGSALAAGGFSATTMLFSLG
                     ASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSR
                     RISQDISTRDEGGGGKVVVIGRQG"
BASE COUNT          262 a          418 c          309 g          308 t
ORIGIN      
        1 aactgcgagg tggtcttgcc gttaatggtg acatcctttt ccgtcatcca tttaccacca
       61 cgctcaagct gaatcagcag gcggacagac gtcgggttac ggtcaccctt tgacgtggtc
      121 tccaccagtg actgcacccc gaaggtcacc cgcaggcggt caatgttcgc tgacgtaatg
      181 gtgcgtgtca ccggttttgc cttcgtcact tccacaccca gtacagtttc agctccggag
      241 gactcaaagc cttccggtgg tgtctgctcc tgctccccgg cgcgccagac cgccgtcaca
      301 ccgtgtatca cgggattacc gtccgtgtcc gtcagcgggg ttttgttcac cagaatactc
      361 tgcagtccct tcaccggacc ttccaccggt ccctcaccaa tggcatcaat cacgctcatc
      421 atctgcgtgg atttgagatt atccttcgcc tcacgaggtg tgtgcgcctt gccgccacct
      481 ttacccactc tgtccccctc tcctgtctga tgtctgaatc tgtttatgcc caaaaaacaa
      541 caggcacccc ggagggtgcc tgcatcatga cggaataaaa attctgaata tcttcacatt
      601 ttcacaaact gactgtggcg cgtataattt cgctgcgtta gtgttttttt gccgtgacat
      661 aaaaaaacaa ctccttaaca ctaatcttca tttgtctgaa tctcccgcag ctccgcgatc
      721 actgcgggat tttttattct ttttacccct gccgtccgat aaccaccact ttcccgcccc
      781 cgccttcatc acgggtactg atgtcctggg atatacggcg ggagccaacc agcatttccc
      841 cgtaaggcac cggcatcggg ttaccctggg caatcatgtt gtccagtgac gaaaagtacg
      901 tgttctgtct gccgttatcc gttgcgcggt aatccggtgt ttttgccttc ggggccagca
      961 tctgggccac accgcccaga atcatactgg cacccagtga aaacagcatc gtggtggcag
     1021 aaaaaccacc ggctgccagg gctgaacccc ataacgccat cgtggctccg gcagtgaaga
     1081 aagagcccac gatggccgcc gcccccagca caatctgcag tccgcccttt ccggcaccgg
     1141 ccagtcgcgg cacaatatgg atgaccgttc cctcacccag tggttcgtga aggcgggcat
     1201 acaccgcctc cggtgccgtg tcatcaccgg caatacgtat ctggtaccag ccttcgttca
     1261 tctgacggcg aaagcccggc acctgcagcg acagggc
//
LOCUS       DAZFFE010000271         1265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFFE010000271 DAZFFE010000000
VERSION     DAZFFE010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.274"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..411
                     /locus_tag="ACHJ69_004952"
     CDS             <1..411
                     /locus_tag="ACHJ69_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5017723.1"
                     /translation="SQVARETRVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKL
                     GIRMLGRRPNSILKAGQHRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPL
                     KQAFDENVDRIRRERLPKELAYALKQQLRIAIKR"
     gene            408..803
                     /locus_tag="ACHJ69_004953"
     CDS             408..803
                     /locus_tag="ACHJ69_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO5017724.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTEIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            811..>1265
                     /locus_tag="ACHJ69_004954"
     CDS             811..>1265
                     /locus_tag="ACHJ69_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO5017725.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWTSTGQGQKSAGDTSFTLAWKPGEEGQKGLIAWFES
                     GDVRAYKIRFPNGTVDVFSGWVSSIGKAVTAKEVITRTVKVTNVGRPSMA"
BASE COUNT          325 a          283 c          392 g          265 t
ORIGIN      
        1 tcacaggttg cccgggaaac cagggtgaga cggaaactgg taaaggaacg gtccagactg
       61 aaacgggcga cggtcagaaa tccgaatgcc agaattatcg ttaaccgcgg tgatctccct
      121 gtgattaagc tggggatcag gatgctgggg cgtcgcccga acagcatact taaagccggt
      181 cagcatcggt atcagcgggc atttattcag cgattaaaaa atggtcgctg gcatgtcatg
      241 cagcgtgtgg ccgggaaaaa ccgttacccc attgatgtgg tgaaaatccc gatggcggcc
      301 ccactgaaac aggcatttga tgagaatgtt gaccgtatcc ggcgtgaacg cctgcctaaa
      361 gaactggcat acgcgctgaa acaacaactg aggattgcaa taaaacgatg aaacacactg
      421 acattcgtgc cgcagtgctg gatgcactcg agcagcatga acacggggcg acgctgtttg
      481 atggtcgccc cgttgttttt gacgaagagg attttcctgc gatcgcggtt tatctgacgg
      541 atgcagagta taccggtgaa gagctggatg cagatacctg gcgggccacg ctgcatattg
      601 aggtgttttt accggcacag gtaccggatt cagagcttga tcagtggatg gaaagccgga
      661 tttacccggc gatgaccgag atcccggcac tggcaggact gattaccacg atggttacgc
      721 agggctatga gtatcgtcgt gatgacgata tggcgttatg gagttctgca gatctgactt
      781 attccattac atacgagatg tgaggacgat atggcaacac caaatcccct tgagccggta
      841 aaaggtgccg gtaccactct gtgggtttac aacggcaagg gtgatgctta tgcaaacccg
      901 ttgtcagacg atgactggca gcgactggct aaggtgaagg atctgacgcc gggcgagatg
      961 acggcagaat cctacgatga taactacctg gatgatgaag acgcggactg gacctcgacc
     1021 gggcagggac agaaatctgc aggtgatacc agttttacgc tggcctggaa accgggtgag
     1081 gaaggccaga aagggcttat cgcatggttt gaaagcgggg atgtcagggc ctataaaatc
     1141 cggttcccga atggtacggt ggatgtgttc agtggctggg tcagcagtat cggtaaggcc
     1201 gtgacggcga aggaagtgat caccagaacg gtgaaagtta ccaatgtggg acgcccgtcg
     1261 atggc
//
LOCUS       DAZFFE010000272         1260 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFFE010000272 DAZFFE010000000
VERSION     DAZFFE010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.275"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            95..1069
                     /gene="cysB"
                     /locus_tag="ACHJ69_004955"
     CDS             95..1069
                     /gene="cysB"
                     /locus_tag="ACHJ69_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO5017726.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELTQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT          331 a          289 c          286 g          354 t
ORIGIN      
        1 agttagcacc ttttttatta ttaaatcgta ttagtcaccc gcctggtgtg acgaaaaaac
       61 gatgttctga tggcgtctaa gtggatggtt taacatgaaa ttacaacaac ttcgctatat
      121 tgttgaggtg gtcaatcata acctgaatgt ctcatcaacg gcggaaggac tttacacatc
      181 acaacccggg atcagtaaac aagtcagaat gctggaagac gagctaggca ttcaaatttt
      241 ttcccgaagc ggcaagcacc tgacgcaggt aacgccagca ggacaagaaa taattcgtat
      301 cgctcgtgaa gttctgtcga aagtcgatgc cataaaatcg gtcgccggag agcacacctg
      361 gccggataaa ggctcgctgt atatcgccac cacgcatact caggcacgct atgcgttacc
      421 aaacgttatc aaaggcttta ttgagcgtta tcctcgcgtt tctttgcata tgcaccaggg
      481 ctcgccgaca caaattgctg atgccgtctc taaaggcaat gccgatttcg ctatcgccac
      541 ggaagcgctg catctgtatg aagatttagt gatgctaccg tgctaccact ggaatcgggc
      601 tatagtagtc actccggatc acccgctggc aggtaaaaaa gccattacca ttgaagaact
      661 gacgcaatat ccgttggtga catatacctt cggctttacc ggacgttcag aactggatac
      721 tgcctttaac cgcgcagggt taacgccgcg tatcgtcttc acggcaacgg atgctgacgt
      781 cattaaaact tacgtccggt tagggctggg ggtaggggtc attgccagca tggcggtgga
      841 tccggtcgcc gatcccgacc ttgtgcgtgt tgatgctcac gacatcttca gccacagtac
      901 aaccaaaatt ggttttcgcc gtagtacttt tttgcgtagt tatatgtatg atttcattca
      961 gcgttttgca ccgcatttaa cgcgtgatgt cgttgatgcg gctgtcgcat tgcgctctaa
     1021 tgaagaaatt gaggtcatgt ttaaagatat aaaactgccg gaaaaataat ttcttgcgtt
     1081 attttcggca ccttttatgt agcgaaggtg ccggaatata ttctcctttg ttacttattt
     1141 gtatccggtg cacaatattt ttatccctca tatttatagg gtaaattacc tgtaaagtag
     1201 tgtctaatta cacacctcgc aaactccagt tttcaaatat ctatttcagg tcaaaagatt
//
LOCUS       DAZFFE010000273         1233 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFFE010000273 DAZFFE010000000
VERSION     DAZFFE010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.276"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            263..>1233
                     /gene="aslA"
                     /locus_tag="ACHJ69_004956"
     CDS             263..>1233
                     /gene="aslA"
                     /locus_tag="ACHJ69_004956"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFO5017727.1"
                     /translation="MEFSFSPKLLVVAVAAALPLIASAADTPSTATARKGFAGYAHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYG"
BASE COUNT          327 a          322 c          286 g          298 t
ORIGIN      
        1 ttaataacat atatattaat tgccgttaaa actaaaaaca gcatcaataa tcaacgcgat
       61 ataacatcac cgccttacat atcacctgcg ccagaggtag gattgcaaac gctctcctga
      121 ttttccaatt cattttctgg ataaataaat aatttatttt tatcactatt atttatgcaa
      181 tcatcctgtc agggagaggg atctcaatta tcaatgctta attacgtcat cattttgacg
      241 acatgaaacc ggagcatttt gcatggaatt ttcgttttca cccaaacttc ttgttgttgc
      301 tgtcgccgca gctcttcctc tcatagccag cgcagcagat accccgtcaa ccgccaccgc
      361 acgcaaaggc tttgccggat acgctcaccc aaaccagtat ctggttaaac cggcgaccac
      421 tattgccgac aacatgatgc cggtgatgca gcatcctgca caagacaaag aaacccagca
      481 gaaactggcg gaacttgaga aaaaaaccgg taagaaaccg aacgtggttg ttttcttgct
      541 ggatgatgtg ggctggatgg acgttggctt taacggcggc ggcgtggcgg tgggtaaccc
      601 tacaccagat atcgacgccg ttgccagcca ggggctgatt ttaacttcgg catattctca
      661 accgagttct tccccaaccc gcgccacgat tctcaccgga caatactcca tccaccacgg
      721 cattctgatg ccgccaatgt acgggcaacc gggcggtctg caagggttaa ccacgctgcc
      781 gcagttgctg cacgatcagg gctacgtcac ccaggctatc gggaaatggc atatggggga
      841 aaacaaagag tcgcagccgc agaacgttgg ctttgatgat ttccgcggct ttaactcggt
      901 atctgatatg tataccgaat ggcgtgatgt tcatgtcaac ccggaagtcg ccctgagccc
      961 ggatcgttct gaatacatcc aaaaactgcc gttcagcaaa gatgacgttc atgccgtgcg
     1021 cggcggtgaa caggaagcca ttgccgacat tacgccgaaa tatatggaag atctggatca
     1081 acgctggatg gaatatggcg ttaagttcct cgacaagatg gcgaagagtg ataagccttt
     1141 cttcctctat tacggcactc gcggctgtca cttcgataac tacccaaatg cgaaatatgc
     1201 gggtagctct ccggcgcgca cctcttacgg cga
//
LOCUS       DAZFFE010000274         1219 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFFE010000274 DAZFFE010000000
VERSION     DAZFFE010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.277"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(163..856)
                     /locus_tag="ACHJ69_004957"
                     /pseudo
     CDS             complement(163..856)
                     /locus_tag="ACHJ69_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          363 a          266 c          254 g          336 t
ORIGIN      
        1 atgtatataa aaaaactatt acgcaatcat tagatacccc cagatttagt tgtaaagcaa
       61 gcacactttg catttcgaga caatccaggt aaagaccctt acctcccatt ttgtgatttt
      121 tatacttatg atgataagtt gaacgttggg tagtgcttcc aatcagtaga acatgtgctt
      181 ttcaatgaag gaaccgatga ctttttcgtg gattgcaacg gaacgcgaga agcagattgt
      241 tttacttacc agccgcttga tgcgggtgcg gagggtcagg ttgttacgtt caatacgttg
      301 cgtgaagatt ttgccggtca ggtatttctc cttcggcact tctctggcgt agctgcccca
      361 gtcatcgcgg ccaccatgcc gatacagaac ggagtcaaca gggctaacaa ttcccgacag
      421 gcctcatcat tccgtggccc gaaagtgtaa gccaacacac cgccagtttt agtgttctac
      481 gcataccaga gccagtgttg ttgcgccttg ttgccaacga agctccacag ctcatcaagt
      541 tcacagataa gggcaacatc agcgtgagct gactgacgca gatgttatcc gctggggcga
      601 gagcttttta atgtccgtat gacggtattg atgctgattt tcagtgttct ggcgatatca
      661 cggcccctgt gccattatgc gccatttcaa cgatcagctc cttaaagccg ggttttctgg
      721 cttcataaga gtaagtgagc tggaaaacac gtttacatga acgacaacgg aaacgctcat
      781 gctgggaaca actacgacca tgtcgataaa ctttatcgga attacagcgc ggacaatgaa
      841 cagtaacggt agccatggaa aaccttaaat accagtagat taccagaaac ttctatcaat
      901 tgaagacatt accgtttttg attacagcaa atgttttatt aattacccca ccatcgacta
      961 aatccgttag aacgtcgaat gatgccattc gtaaatacat taaaaagact gttgtaatca
     1021 acgaagatgg aaaacagtgt gttaaaacta atctgaactt cagaaaaaca gatgatggta
     1081 tggtgatata tatatccgct ctgaaccgcc acgcaattta aaataaaaat caacaaatat
     1141 cgtcgatatc catatatacc atttttctgt agttgtatta gcgtcgcata ttttatgact
     1201 ttaagtcata agtcaaaac
//
LOCUS       DAZFFE010000275         1218 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFFE010000275 DAZFFE010000000
VERSION     DAZFFE010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.278"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            71..>1218
                     /locus_tag="ACHJ69_004958"
     CDS             71..>1218
                     /locus_tag="ACHJ69_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO5017728.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFAS"
BASE COUNT          289 a          262 c          312 g          355 t
ORIGIN      
        1 tttgttgtcg tgataatgac aatcaataaa ggaattctgt atgagtcata cagaaagaac
       61 aggatattaa atgaataaaa aatcgttatg gaagctaatt ctgatattag cgatcccatg
      121 tattattggc tttatgccag ctccggcagg attaagcgaa ctggcgtggg tgctttttgg
      181 tatttacctg gcggccattg tggggctggt tatcaagcct ttcccggaac ctgtcgtact
      241 gttaattgcc gtcgccgcat cgatggtagt ggtgggtaac ttatcaggtg gggaatttaa
      301 aaccaccgcc gtattaagcg gttactcttc cggtaccacc tggctggtgt tttctgcgtt
      361 tactttaagc gccgcgtttg taaccacagg cttaggtaaa cgtattgcct atctgctgat
      421 tggtaaaatt ggtagcacta ccctgggtct gggttacgtt acggtatttc tcgatctggt
      481 actggctccg gcaacaccgt ctaacaccgc gcgtgcgggc ggcatcgtgt taccgatcat
      541 caacagcgtg gcagtggctt tgggatcaga accggaaaaa agtccgcgtc gtgttggaca
      601 ttacctgatg atgtccattt acatggtcac taaaaccacc agctatatgt tctttaccgc
      661 aatggcgggg aacattctgg cgctgaaaat gatcaacgac attctgcacc tgcaaattag
      721 ctggggtgga tgggcgctag ccgccggatt gcctggcatc attatgctgc tggtcacccc
      781 gctggtgatt tacaccatgt atccaccaga aattaagaag gtggataaca aaaccatcgc
      841 taaagcgggc cttgccgaac tgggaccgat gaaaatccgc gaaaaaatgc tgctcggtgt
      901 ctttgtgctg gcgctgctgg gctggatttt cagtaagtct ctgggggttg atgaatccac
      961 cgtggcaatc gttgttatgg caactatgct gctgctgggt atcgttacct gggaagacgt
     1021 ggttaaaaat aaaggcggct ggaatacctt aatctggtac ggcggtatta tcggcttaag
     1081 ctccttatta tcgaaagtta aattcttcga atggttagct gaagtcttta aaaataacct
     1141 ggcatttgat ggtcacggta acgttgcttt cttcgttatt attttcctca gcatcatcgt
     1201 gcgttatttc ttcgcttc
//
LOCUS       DAZFFE010000276         1206 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZFFE010000276 DAZFFE010000000
VERSION     DAZFFE010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.279"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(157..774)
                     /locus_tag="ACHJ69_004959"
     CDS             complement(157..774)
                     /locus_tag="ACHJ69_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017729.1"
                     /translation="MYGLSITKPDGSLWISPGFTPQCLINKGTIPATEKAFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHQIVRGTNPGVTTVYAFANMVTQPSEY
                     GIAMYNPSGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINSSIYD"
     gene            complement(780..1061)
                     /locus_tag="ACHJ69_004960"
     CDS             complement(780..1061)
                     /locus_tag="ACHJ69_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017730.1"
                     /translation="MAEYGFAIYNRNNVNVTGVLTPIFFLDRFTAESGSKTYTNKPEG
                     KTLQAVCSLFPWNNVFKDRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            complement(1076..>1206)
                     /locus_tag="ACHJ69_004961"
     CDS             complement(1076..>1206)
                     /locus_tag="ACHJ69_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5017731.1"
                     /translation="VTSQGQLTIRYSNQNVGLVIRNDKIEVYDQNGRLAVRIGRLS"
BASE COUNT          342 a          287 c          214 g          363 t
ORIGIN      
        1 gatgctgcat ttacctgtgc agttaagcaa ctcatcacca gaactgcaca cgcaaaaaat
       61 ttcatgatac ttttcatttt aagtatttct ctgaattaaa ggatgcgatg tctgaaagga
      121 acgctttcgt tttacactat taaagttttt cttacgctaa tcatatattg aggaattaat
      181 aaccagaact ttatctatat acggctttcg gatatcccat gttgcaccac cggaataatg
      241 ctcacaggaa tatattttat ttcctgtagc accagtggat gtggtgtaaa tcggtcggtc
      301 ataaggtgtt cgtttccagt tataatatcc aaccagtgca ggcataattg cgcatggata
      361 tccaaggtcc ttctcaaact tgatatcaac tggtattaac ttcgcgtcaa gcagcatcat
      421 ttcgccatgg taaatcatct cacccgacgg gttatacatg gcgataccat actcagaagg
      481 ttgagtaacc atattcgcaa aagcataaac agtcgtaaca ccgggattcg ttcccctgac
      541 aatttgatga agccttaagg catgatatcc atcaatttgt tcatgcgtgt acataacatc
      601 ggcctttttc tctgttctga taaagaaaaa acaacttttt cctgacggga ttgacgtttt
      661 aaaaaaagcc ttctcagtcg ccggaatagt tcccttattg atcagacact gaggcgtaaa
      721 ccccgggctt atccacaagc tgccatctgg tttcgtaatg cttaaaccat acatacaact
      781 cacccccaga atgtataaat atatgacccc atcccctgct caagattcga ccatgtcacc
      841 gtattgccat taatggttat cttcggtact ttccggtcct taaatacatt attccacgga
      901 aataatgaac agactgcctg taatgttttc ccttctggtt tattcgtata cgtttttgat
      961 cccgactccg ccgtaaacct gtccaggaaa aatatgggag taagcacgcc cgtaacatta
     1021 acattatttc tgttgtatat tgcaaaacca tattctgcca atgtcagcct ccggctcagc
     1081 ttaacctgcc tatgcgaaca gccaggcgtc cgttctggtc ataaacttca attttatcat
     1141 tgcggatcac cagccccaca ttctgattag agtaacgaat tgtcagttgc ccctgcgacg
     1201 taacac
//
LOCUS       DAZFFE010000277         1177 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZFFE010000277 DAZFFE010000000
VERSION     DAZFFE010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.280"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..390)
                     /locus_tag="ACHJ69_004962"
     CDS             complement(<1..390)
                     /locus_tag="ACHJ69_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HFO5017732.1"
                     /translation="MSFLDAKSYDTLFGLPELYEDALPRFSLSCEDIAFARTHRRSHN
                     RLGFAIQLALVRDLGRTLRAGEIPPEAVITVVAEQLDIDPAVFTLYAQREETRREHAR
                     EIVSALELRSVRSSDYRMLIIAATREAA"
     gene            complement(371..994)
                     /locus_tag="ACHJ69_004963"
     CDS             complement(371..994)
                     /locus_tag="ACHJ69_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO5017733.1"
                     /translation="MLIGYMRVSSNDERQSVSLQRDALIAAGVDPRHLHQDHASGARD
                     NRPGLKSCLAELREGDVLIVWKLDRLGRSLSHLLRIIEDLKIRGIAFRSLTESIDTTT
                     PHGSFLFNLFGTLAEYERTLITERVNAGLAAALRRGRKGGRPPAIDAEKVDQILAALE
                     GGASKASVCRAFSVPRSTLTDTLRRIDWAGSGGRNLTTDQSNVISRR"
BASE COUNT          323 a          297 c          284 g          273 t
ORIGIN      
        1 tgctgcttcg cgtgtagccg cgataatgag cattctgtaa tcgctactcc ggacggaacg
       61 aagctccaaa gccgaaacta tttcgcgggc atgttcccgg cgggtttcct cacgttgtgc
      121 gtaaagtgta aaaacagcag ggtcaatatc cagttgttcc gcaacaacgg tgataacagc
      181 ctccggggga atctctcctg cacgcagtgt acgaccgaga tcacggacaa gtgcaagctg
      241 aatggcaaaa cccagacggt tatgagatcg acgatgagtt ctggcgaatg caatatcctc
      301 gcaggagagc gaaaaccggg gaagtgcatc ctcataaagt tcaggtaatc caaaaagcgt
      361 atcgtacgat ttagcgtcga gaaatgacat tgctctgatc cgttgtcaga ttacgccccc
      421 cagatccggc ccagtcaatt cgtcgtagag tatcggtgag cgttgagcgt ggcacactga
      481 atgcgcgaca tactgacgct ttactggcac ctccttccag agctgcgagg atttgatcaa
      541 ccttttcagc atctatcgcc ggtggccttc cgccttttct cccccgccgg agagcagcag
      601 ccagaccagc attaacgcgt tctgtgataa gtgttctttc gtactccgcg agcgtgccaa
      661 aaaggttaaa gaggaacgag ccgtgtggcg ttgttgtatc aatagattca gtcaatgatc
      721 ggaaagcaat accacgaatt ttcaggtctt caataattcg gagcaaatgt gagagtgaac
      781 gaccaagtcg atccagtttc cagactatca gtacatcacc ttcgcgaagt tcagcgagac
      841 aggacttaag tcccgggcga ttatcacgag cacctgaagc atgatcctga tgtaaatgcc
      901 ggggatccac tccagcagca atcaacgcat cacgctgcaa agaaactgac tgacgttcat
      961 cattgcttga tacccgcata taaccaatca acatgtacga caaaccttaa aattgaagtt
     1021 tccgtacagt ttctcattcc gacagggttt gtcacgcaaa aaaatcacag atttatggat
     1081 tcaattttct tcaactgcag gacctgacgg aaaacaagtg atttccgtca gggaggaatc
     1141 gcttagcggg tgttatcgca gaaattccca cggaacc
//
LOCUS       DAZFFE010000278         1159 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFFE010000278 DAZFFE010000000
VERSION     DAZFFE010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.281"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..569
                     /locus_tag="ACHJ69_004964"
     CDS             <1..569
                     /locus_tag="ACHJ69_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO5017734.1"
                     /translation="QSHLTRELPAILEDGENGLSPFVRTSIYRQSKHIRELEEQVKQV
                     EEALASWYRTQEACQRMAKIPGGGMLTATYVVAAVGNARQFSTAKQFASWLGLTPKEH
                     SSGGKQQLGGISKRGDGYFRYLLVHGARALTAWVNRNGAVEENSWLQGLLERKHYNVA
                     VVAMAAKTARIMWSMLSHNTEYQPRQLA"
     gene            complement(1071..1154)
                     /gene="yohP"
                     /locus_tag="ACHJ69_004965"
     CDS             complement(1071..1154)
                     /gene="yohP"
                     /locus_tag="ACHJ69_004965"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO5017735.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT          322 a          280 c          318 g          239 t
ORIGIN      
        1 gacaaagcca tttaacccgc gagctgccag ccattctgga ggatggcgaa aatggtttat
       61 ctccctttgt cagaaccagc atctacagac agtctaaaca tatccgggaa cttgaagaac
      121 aagttaaaca ggtagaagaa gctctggcct cctggtatag aacgcaggaa gcctgccaga
      181 gaatggccaa aatcccgggg ggtggcatgt taacggccac ttatgtggta gcagcagtgg
      241 gtaatgcccg acaattcagt accgcaaagc agttcgcttc atggctgggg ctgacaccaa
      301 aggaacattc cagcggcggg aaacagcaac tgggagggat cagcaaacgt ggagatggat
      361 atttccgata cctgctggtt cacggcgcac gcgcacttac cgcctgggtc aaccgaaacg
      421 gcgcggttga ggagaattcc tggcttcagg gactccttga gcggaagcac tacaatgtgg
      481 ctgttgtcgc catggcggca aaaacagcga ggatcatgtg gtcaatgttg tcacacaata
      541 ctgaatatca acctcggcag ctcgcctgac acaaggtcag tcaatctgac gaggtgctga
      601 aaaaaagtta acgtcctggt aggtgcaggt gacttcagaa gatggcaaaa caggtaaggc
      661 cgcaggcagg atacaccgag aggtaacaag ggcaagttag cccgtagagg atgattggta
      721 cctgcctggc ggatttcatc atggttcggg ataaaaatcc catcagaaac cggatatatg
      781 gctgcaccta cccgttttat tacataactg aagaaagcat tgcattccgg gagcgtccat
      841 atatgttacc tcgcgcccga cccggtagcc gtaagggata agccgagtgc accgcgcagc
      901 ggcgattttg ttcgccagag cccggggggg cagggggcgg cggcgactgg tcgccccctg
      961 cgcgctcctt gcgccagtga ttatatgaag caatgcacat gacaggagcg caacaggatg
     1021 agaggctgaa ctattctcgt tattttcgga cctgatgaca ttaattaatt ttaaaatatc
     1081 atcttaaata cgccagtcac caccagtagc ccaatcagga aaataatcaa tacagcccag
     1141 agtataattt tcattggcg
//
LOCUS       DAZFFE010000279         1151 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZFFE010000279 DAZFFE010000000
VERSION     DAZFFE010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.282"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            71..>1151
                     /locus_tag="ACHJ69_004966"
     CDS             71..>1151
                     /locus_tag="ACHJ69_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFO5017736.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
BASE COUNT          332 a          237 c          262 g          320 t
ORIGIN      
        1 attgattaga tgaatatttg tagtgcagtg acatcatttt taaataatag ttcaaataaa
       61 agggctcacg atgaaaaaat taacagtggc aatttctgct gtagctgcat cagtactgat
      121 ggcgatgtct gctcaggcag cagagattta caataaagac agtaacaagc tggatctgta
      181 cggaaaagtt aatgctaagc actacttctc ttctaacgat gcagatgatg gcgacacaac
      241 ttatgttcgt ctgggcttca aaggcgaaac tcaaatcaac gatcaactga ctggtttcgg
      301 tcagtgggaa tatgaattca aaggcaaccg tgctgaatct caaggttctt ccaaagataa
      361 aacccgtctt gccttcgctg gcctgaaatt cggtgactac ggctccatcg attatggccg
      421 taactacggt gtagcatacg atatcggtgc atggaccgac gttctgccag aattcggtgg
      481 cgatacctgg acccaaacag atgtgttcat gactggtcgc accacaggtg ttgcaaccta
      541 tcgtaacaac gacttctttg gtctggttga tggtctgaac tttgctgctc agtatcaggg
      601 taaaaatgac cgcactgacg taactgaagc taacggtgat ggtttcggtt tctccactac
      661 ttatgagtat gaaggattcg gtgtaggtgc aacctatgct aaatctgacc gcactaatga
      721 tcaggttatc tacggtaaca acagcctgaa tgcatctggt caaaatgctg aagtatgggc
      781 agctggtctg aaatatgatg cgaacaacat ctatctggct accacctatt ctgaaaccca
      841 gaacatgact gtttttggta ataaccatat tgccaacaaa gcacaaaact tcgaagtagt
      901 tgcacaatat cagttcgact tcggtctgcg tccgtccgtt gcttacctgc aatctaaagg
      961 aaaagacttg ggtgcgtggg gtgatcagga cctggttgaa tatattgatg taggtgcaac
     1021 ctattacttc aacaaaaata tgtccacttt tgttgattac aaaatcaacc tgattgataa
     1081 gagcgatttc acgaaagcat ctggcgttgc taccgatgat atcgttgctg taggattggt
     1141 ttaccagttc t
//
LOCUS       DAZFFE010000280         1151 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFFE010000280 DAZFFE010000000
VERSION     DAZFFE010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.283"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..839)
                     /locus_tag="ACHJ69_004967"
     CDS             complement(<1..839)
                     /locus_tag="ACHJ69_004967"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO5017737.1"
                     /translation="MAWNDELFMPCNINHGALCPTFLSGGSVSEKVNKTSLIHDAAFR
                     KTLKDPAAARDFLEQVLTPYQKSRCNLDTIELESTTFVAESLRQSACDVLLSMKTNDG
                     KDGYIYTLIEHQSSPDKFIPLRMMRYILAVMEQHIEEHKCAPVVIPVLFYHGAKRPYP
                     YPMNWVDCLDDPVYGREIYGEQKPFSLVDVSTLTDDEIEHYHRMAALMFTMKSGTSGD
                     VIELIGKSITLTDKYGSSVHQNIVLTYLMELYQMDFAELSEAVSTHYPIHKGVIMTIA
                     EQL"
     gene            complement(805..>1151)
                     /gene="ssb"
                     /locus_tag="ACHJ69_004968"
     CDS             complement(805..>1151)
                     /gene="ssb"
                     /locus_tag="ACHJ69_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5017738.1"
                     /translation="YLRKGSKVYIEGQLKTRKWFDQQNGVDRYSTEVVVGVNGVMQML
                     GSLRQQGTAGNGASAPAQGGWGQPPKTAGQAGRAPQTTSTGNGAPPMDFEDDIPFMGL
                     GYGMERRIIHAM"
BASE COUNT          282 a          320 c          250 g          299 t
ORIGIN      
        1 tccagttgct ctgctatcgt cataatcacc cccttgtgga tgggatagtg tgtggatact
       61 gcttcagaaa gttcagcaaa atccatctgg taaagttcca tcagataagt cagcacgata
      121 ttctggtgaa cgctgctgcc atacttatcc gtcagtgtga ttgatttacc aatcagctca
      181 atcacatcac cactggtgcc ggacttcatc gtgaacatca gcgccgccat ccggtgatag
      241 tgttcaattt catcatcggt aagcgtgctg acatccacca agctgaatgg cttttgctcg
      301 ccataaatct cgcgtccgta caccgggtca tccagacaat ccacccagtt catcggatac
      361 ggatatggcc gctttgcacc atgataaaac agcaccggga tcactactgg cgcacactta
      421 tgctcttcaa tatgctgctc catcaccgcc agaatataac gcatcatgcg cagtgggata
      481 aatttatccg gcgaactctg gtgttctatt aaagtataaa tgtacccgtc cttcccgtca
      541 tttgtcttca tgctgagcaa aacatcgcag gcagactgac gtaaactttc tgccacaaac
      601 gtcgtggatt caagttctat tgtatcaaga ttacagcggg atttctgata cggtgtcaag
      661 acctgttcaa gaaagtccct cgcggctgca gggtccttca gtgtcttcct gaacgccgca
      721 tcatgtatca ggctggtttt attaactttt tcactcacgg aacctccgga taaaaaagtg
      781 gggcataacg ccccatggtt gatgttacat ggcatgaata attcgtcgtt ccatgccata
      841 cccgagcccc ataaagggaa tatcatcctc aaagtccatt gggggtgcac cattacccgt
      901 tgatgttgtc tggggtgcac gaccagcctg tccggccgtc ttcggcggct gtccccagcc
      961 cccttgtgca ggtgcagaag caccattacc ggcagtgccc tgctgacgca gtgaacccag
     1021 catctgcatg acaccattca ctcccacaac aacctcagtt gaataacggt ccacaccgtt
     1081 ctgctggtca aaccacttac gggttttcag ctggccttcg atatagacct tagagccctt
     1141 acgaagatac t
//
LOCUS       DAZFFE010000281         1140 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFFE010000281 DAZFFE010000000
VERSION     DAZFFE010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.284"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..555
                     /locus_tag="ACHJ69_004969"
     CDS             <1..555
                     /locus_tag="ACHJ69_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5017739.1"
                     /translation="ALSMQVPGFRRQMNEGWYQIRIAGEDTAPEAVYARLHEPLGEGT
                     VIHIVPRLAGAGKGGLQIVLGAAAIVGSFLTAGASMALWGTALSAGGFSATTMLFSLG
                     ASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSR
                     RISQDISTRDEGGGGKVVVIGRQG"
     gene            897..>1140
                     /locus_tag="ACHJ69_004970"
     CDS             897..>1140
                     /locus_tag="ACHJ69_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017740.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAVGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGF"
BASE COUNT          273 a          259 c          367 g          241 t
ORIGIN      
        1 gcactgtcga tgcaggtgcc gggctttcgc cgtcagatga acgaaggctg gtaccagata
       61 cgcattgccg gtgaggatac ggcaccggag gcggtgtatg cccgccttca cgaaccactg
      121 ggtgagggaa cggttatcca tattgtgccg cgactggccg gggccggaaa gggtggactg
      181 cagattgtgc tgggggcggc agccatcgtg ggctctttcc tcactgccgg tgcctcgatg
      241 gcgttatggg gtacagccct gagtgccggc ggtttttctg ccaccacgat gctgttttca
      301 ctgggggcca gcatgattct gggtggtgtg gcccagatgc tggccccgaa ggcaaaaaca
      361 ccggattacc gcgcaacgga taacggcaga cagaacacgt atttttcgtc actggacaac
      421 atgattgccc agggtaaccc gatgccggtg ccttacgggg aaatgctggt tggctcccgc
      481 cgtatatccc aggacatcag tacccgtgat gaaggcgggg gcggaaaggt cgtggttatc
      541 gggcggcagg ggtaaaaaga ataaaaaaaa tcccgcagtg atcgcggagc tgcggggaca
      601 gacaaagatt agagttaagg agttgttttt gttactcggg caaaaaaaca ctaacgcagc
      661 gaaattatac gcgccacagt cagtttgtga aaatgtgaag atattcagaa tttttatgcc
      721 attaccggtt tttaccaaca ggattatcgg tggacatgaa agaaaacccc ggtatctgct
      781 gataccgggg tttctcttta gcatggcaga aatgtgtttc atgcttttcg ggcgaaggat
      841 atccgacttc tgtacggaat ggcaagtggc ggttaattta ttcaggggaa ggctgtatgg
      901 gaaaaggtgg cggtaaggca cacacgcctc gtgaggcgaa ggataatctc aaatccacgc
      961 agatgatgag cgtgattgat gcggttggtg agggaccgat agaaggcccg gtgaagggac
     1021 tgcagagtat cctggtgaac aaaaccccgc tgacggacac ggacggtaat cccgtgatac
     1081 acggtgtgac ggcggtctgg cgcgccgggg agcaggagca gacaccaccg gaaggctttg
//
LOCUS       DAZFFE010000282         1071 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFFE010000282 DAZFFE010000000
VERSION     DAZFFE010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.285"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            202..630
                     /gene="tolA"
                     /locus_tag="ACHJ69_004971"
     CDS             202..630
                     /gene="tolA"
                     /locus_tag="ACHJ69_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO5017741.1"
                     /translation="MYCRAVPVLVLILASLTTGCTKDVSTNNYDASLYHSDKLIKSQN
                     LSSSERTPDASEEIRQYAIQVRKAIEEQLKDASKYSGKECSLRMYMAPNGLLLQVKRE
                     SGDPDLCREAMNAVKNADIPSPPSSGVYKAFRNGVLDFKL"
     gene            889..>1071
                     /locus_tag="ACHJ69_004972"
     CDS             889..>1071
                     /locus_tag="ACHJ69_004972"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017742.1"
                     /translation="MQWHIAPLSSPSPEGLSCGQKKSPSGFGQQHEIYIFIIEWILTR
                     IHKAAQLRGLFRIAGCS"
BASE COUNT          302 a          207 c          262 g          300 t
ORIGIN      
        1 tcagacttcc ttgtttattc tcgccggatg cttcgtagat atttgcaggc atctgtatgc
       61 aggggtatta ttgcggcata tatcatccgg aaaaataaaa actacatgct gatccggaga
      121 tgatagttac aagtcctccc ggatcccgca tatttactat attttataga taattgttct
      181 tgttaatatg gagatggata aatgtattgc cgtgctgtac cagttcttgt tttgatatta
      241 gccagtctga caactggctg cacaaaagat gtatcgacaa ataattatga tgcttctctg
      301 tatcactcag ataaattaat aaagtcgcaa aatttgagct catcggaacg tacccctgat
      361 gccagtgaag aaatcaggca gtatgccatt caggtcagga aagccataga agaacagtta
      421 aaggatgcga gtaagtattc aggaaaagag tgttcactga gaatgtatat ggccccgaat
      481 ggtcttctgc tacaggttaa aagagaaagc ggtgatcccg atttatgtcg tgaagcgatg
      541 aatgcagtaa agaatgctga tataccatca ccaccgtctt caggagtata taaagcattt
      601 agaaatggcg tgctggattt taaactctga cagggggaga agattttctc ctgtcctgat
      661 gattttgggg cgctggaaag atattgtgct gtgaagtgtt aaattcctca caattcagta
      721 agttgacagt tgcctgtcag actgagcatt tgttaaaaaa atttcgcatg gtgaatcccc
      781 ctgtgcggag gggcgactgg tgaacggtat gatctctttg atgatcgtaa gcgagaatat
      841 gcgggtttag tgtcaccggg ctgaactcac cgggaggcac ccggcaccat gcaatggcac
      901 atagcgccac tctccagccc ctctccggag gggctttctt gtgggcaaaa aaaaagcccg
      961 agtgggttcg ggcaacagca tgagatatac atttttataa tcgaatggat tttaaccaga
     1021 attcataagg ctgcgcaact gcgcggcctt tttcgtattg cgggctgtag t
//
LOCUS       DAZFFE010000283         1068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFFE010000283 DAZFFE010000000
VERSION     DAZFFE010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.286"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..316)
                     /locus_tag="ACHJ69_004973"
                     /pseudo
     CDS             complement(1..316)
                     /locus_tag="ACHJ69_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            342..647
                     /locus_tag="ACHJ69_004974"
                     /pseudo
     CDS             342..647
                     /locus_tag="ACHJ69_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
BASE COUNT          243 a          267 c          314 g          244 t
ORIGIN      
        1 agaggcggtc gaactccgcc ttcgcaaaat aagcggaagc ctggcgaagg atatcgttac
       61 tgcggcgcag ttcacgattt tcacgttcca gctctttcag acgctgacgt tcagcggtgg
      121 tgagcccgcc atcaccgcct ccggtattcc gctcatgctg gcgtacccag acacgcagag
      181 tctccggtgt acagccaatc tttggggcaa tggaacaaat tgtcgcccat tgtgagtcat
      241 attcgccctg actttccaga accatacgga ctgcccgttg acggacttcg ggggaaaaac
      301 gagtattttt agtcatcctg tttacctctt tctcaggaag tttagtctcc aggattcccg
      361 gggcggttca gttttttaag atggtgaggt acttcgggtt ccttgccaac cgggtgtgtg
      421 gagagaagct gccgcaggtg taccgtgctc tggggatgga taaaccggaa gcagtggcga
      481 aagtgtgcta tgcacaaatg gtgaaacagt tcctgagtcg tgacccgttc gaatgcgtgc
      541 tgtgcggcgg ccggatggta taccgccggg ccattgcggg gctgaatgta tcagggctga
      601 agaaaaacgc gcgggatatc agtctgctga ggtatatgcc ggcctgacac aggctaagtg
      661 tgcccgggaa tcggtgcaag tataaaaaac agcctgtttc ggtataaaaa tcgaccatac
      721 agagagagaa accaccggag tggtgggaaa agcagaaagg ctcacgtggc ataaggcgtg
      781 gacaatggta tcgactgttt tttcaatttc ctatacgtcc aggatatgcc gcgccagcgg
      841 catgtaaggc ggcacgctgt ggttacatgt gataccggag taaaaccgac gaagcccggc
      901 gtaagccggt actgattgat agatttcacc ttacccatcc ccagccctgc taagccatac
      961 ctgctttcag ccatgaaaga gcttctgtgc gcggtcggag tggtcccgac tagggtttac
     1021 ccgaagtcgg ggcgtatctc cgcgttagcg ggccgtcagg gccgctta
//
LOCUS       DAZFFE010000284         1037 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFFE010000284 DAZFFE010000000
VERSION     DAZFFE010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.287"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            154..>1037
                     /locus_tag="ACHJ69_004975"
     CDS             154..>1037
                     /locus_tag="ACHJ69_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntS/YbdA MFS transporter"
                     /protein_id="HFO5017743.1"
                     /translation="MQRKTLLSACIALALSGQGWAADITEIETTTGEKKNTNVTCPAD
                     PGKLSPEELKRLPSECSSVVEQNLMPWLVTGAATALITTLAIVELNDDDDDHHRNNSP
                     LPPTPPDDDSDDTPVPPTPGGDEIIPDDGPDDTPAPPKPISFNNDVTLDKTAKTLTIR
                     DSVFSYTENADGTISLQDSNGRKATINLWQIDETNNTVALEGMSADGATKWQYNHNGE
                     LVITGDNTTVNNTGKTIVDGKGATGTEIAGNNAVVNQDGELDVSGGGHGIDITGDSAT
                     VDNKGGMTVTDPDSIGIQ"
BASE COUNT          308 a          236 c          241 g          252 t
ORIGIN      
        1 tttatttctc tgtattataa atccagattg tcagaaaata tgtttgatta ggtaacaatc
       61 tactggcaat attggacgtc ctctatgttt taaataatca attggtcggg tcagggtatc
      121 cgaccttctt tatattcgcc agaaggattt attatgcaaa ggaaaactct attgtcggcc
      181 tgtattgcat tagctctgag tggtcagggt tgggcggcag atattacaga aatagaaacc
      241 accacaggtg aaaagaaaaa taccaatgtg acttgtccag cagatcctgg aaaactcagt
      301 ccagaagagc ttaaacgctt accctctgaa tgctcctctg tagtcgaaca gaacctgatg
      361 ccatggcttg tcacaggtgc tgctactgcg ttaatcacaa ccttagccat agtggaacta
      421 aacgacgatg atgatgatca tcatcgcaat aattctccac ttccaccaac tccacctgat
      481 gatgattcag acgacactcc agttcctcca actcctggcg gagatgagat aataccggac
      541 gacggtccgg atgatactcc tgcacctccc aaaccgattt cgtttaataa tgacgttacc
      601 ctggataaaa cagcaaaaac gttaactatt cgcgattcag ttttcagtta taccgagaat
      661 gctgacggga ctatttctct gcaagatagc aatgggcgta aggcgactat taatctttgg
      721 cagattgatg aaacgaataa cacggttgcc cttgaaggga tgagcgcaga tggtgcaacg
      781 aagtggcaat ataatcacaa cggtgagctc gttattacgg gcgacaatac cacagtaaac
      841 aacactggaa aaaccatcgt cgacggcaaa ggcgccaccg gaactgaaat cgcgggtaac
      901 aacgcggtgg tgaatcagga cggcgagctg gatgtgagcg gcggcggtca cggtattgat
      961 atcaccggcg acagcgcaac ggtggacaac aaaggcggca tgactgtcac cgatcctgac
     1021 tccatcggta ttcagat
//
LOCUS       DAZFFE010000285         1026 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFFE010000285 DAZFFE010000000
VERSION     DAZFFE010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.288"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            6..212
                     /locus_tag="ACHJ69_004976"
     CDS             6..212
                     /locus_tag="ACHJ69_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO5017744.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            209..>1026
                     /locus_tag="ACHJ69_004977"
     CDS             209..>1026
                     /locus_tag="ACHJ69_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5017745.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKA"
BASE COUNT          211 a          239 c          348 g          228 t
ORIGIN      
        1 agtgaatggt tacagtcgct gaactgcagg cgctgcgtca ggcgcgcctt gatttattaa
       61 ccggtaaacg ggtggtgtct gtccagaaag atggtcgcag aattgaatat acggcggctt
      121 ctctggatga gcttaaccgg gcgatcaatg atgcggagtc ggtactgggg acaacccggc
      181 gtcgccgtcg tccgctggga gtgaggttat gaaacgaacg cctgtcctga ttgatgtgaa
      241 cggcgttccg cttcgtgaga gtctcagcta caacgggggc ggtgcaggat ttggcgggca
      301 aatggctgag tggttgccac cggcgcagag tgccgatgcg gccctgctac ccgcgttgcg
      361 tctggggaat gcccgggcag atgatctggt gcgcaataac ggaatagcgg ctaatgcggt
      421 ggctctgcat aaggatcaca ttgtcgggca tatgtttctg atcagctacc gtccgaactg
      481 gcgctggctg gggatgcggg agaccgcagc aaaaagcttt gtcgatgagg tggaggcggc
      541 ctggtcggaa tacgccgaag ggatgtttgg cgagatcgac gtggaaggaa aacgcacgtt
      601 cacggaattt atccgtgaag gtgtgggcgt tcatgcgttt aacggcgaaa tctttgtgca
      661 gccggtctgg gatacggaaa ccacgcagtt attccgtacg cgttttaaag ccgtgagtcc
      721 gaaacgggtg gacacgcctg gacacggtat ggggaaccgt tttctgcggg ccggtgtgga
      781 ggtcgatcga tatggccgtg ccgtcgcgta ccatatttgt gaggatgatt ttccgttctc
      841 tggtagtgga cgatgggaac ggatcccgcg tgaacttccc accggacgtc cggccatgct
      901 gcatattttc gagccggtgg aggacgggca gacccgtggg gctaatcagt tttacagcgt
      961 catggaacgg ctgaagatgc tcgattccct gcaggcaaca cagcttcagt cggccatagt
     1021 gaaggc
//
LOCUS       DAZFFE010000286         1016 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFFE010000286 DAZFFE010000000
VERSION     DAZFFE010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.289"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(129..>1016)
                     /locus_tag="ACHJ69_004978"
     CDS             complement(129..>1016)
                     /locus_tag="ACHJ69_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO5017746.1"
                     /translation="NDDLAALYMLKVQKTKNGIPYVAGIGAGIEDTDGQPLSNILLLA
                     DRIAMINPEDGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPTFSLTPEGRLTA
                     RNADISGHISANSGTLNNVVIAENCTIKGTLRAENIIGDVVKTHNVSLPDLRAAGEHR
                     HATERTVTVIDDQSFDRNIVIPPVFYSGIIYKGSDGDGATSICTLTVSVNDRVVFSKT
                     SSIGTRRTSGGYPVQEDTIYPGSSTPFSYFMRMASGGGDVRIKFRIEVIYYGTAPGFM
                     TTGIQITTMKASASGFVES"
BASE COUNT          234 a          291 c          232 g          259 t
ORIGIN      
        1 ttaacattga agttgactcg gggccttttc acatcaggct ttcaggtgtt ccctccggcc
       61 atcagccaaa ggcacccgcg catactgtgc ggtttttgtc tccttccggc aatcacgatc
      121 gtccgggatt aactttccac aaatcctgaa gctgacgcct tcatcgttgt gatctgtatc
      181 cctgtggtca taaaacccgg tgcagttcca taatagatga cctcaatccg gaacttgatc
      241 ctgacatcac ctccccctga cgccatcctc atgaaatagc tgaacggagt ggatgacccg
      301 ggatatatgg tgtcttcctg aacggggtat ccccctgacg tacgcctggt accgatggat
      361 gaggttttgc tgaacaccac ccgatcattc acactcaccg tcagcgtaca gattgaggtc
      421 gcaccgtcac catcacttcc tttgtagata ataccggaat aaaaaaccgg cgggataaca
      481 atattccggt caaacgactg atcatcaatg acagtaaccg ttcgttctgt cgcgtgtctg
      541 tgctcccctg cagccctgag atcaggaaga ctgacattgt gcgttttcac cacatcgcca
      601 atgatatttt ccgctctcag agtacctttg atggtacagt tctccgctat cacgacatta
      661 ttcagcgtgc ccgagttcgc actgatatgt ccgctgatat ccgcattacg ggccgtcagc
      721 ctgccttccg gcgtcaggga gaacgtaggg ggattgccgg acgaggtgat actcaccgca
      781 aacagccgct tcaggaacac atcgttcatg aacaactgat tcccctgcgc cacaaataac
      841 ggcgtggtgt tgccgtcctc cgggttaatc atcgcaatac ggtcagccag cagcagtatg
      901 ttgctcaggg gctggccatc agtatcctca atccccgctc caataccggc aacatagggt
      961 atgccatttt ttgttttctg taccttcagc atgtaaagtg cagcaaggtc atcatt
//
LOCUS       DAZFFE010000287         1015 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFFE010000287 DAZFFE010000000
VERSION     DAZFFE010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.290"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1006
                     /locus_tag="ACHJ69_004979"
     CDS             <1..1006
                     /locus_tag="ACHJ69_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO5017747.1"
                     /translation="GAGLLAHVVTGKYADHLPLYRQSEIYRRQGVELSRATLGRWTGA
                     VAELLEPLYDVLRQYVLMPGKVHTDDIPVPVQEPGSGKTRTARLWVYVRDDRNAGSQM
                     PPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESGRITEAACMAHARRKI
                     HDVHARAPTYITTEALQRIGELYAIEAEVRGCSAEQRLAARKARAAPLMQSLYDWIQQ
                     QMKTLSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIGNNIAENALRGVAVGRKNWMFA
                     GSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPANRVRDLLPWKVDLSSQ
                     "
BASE COUNT          240 a          269 c          309 g          197 t
ORIGIN      
        1 cggagcgggg cttctggccc atgttgtcac cgggaaatat gcagaccatc tgccgttata
       61 ccgccagtca gaaatatacc gtcgtcaggg agtggagctg agccgtgcca cactggggcg
      121 ctggacaggt gctgttgctg aactgctgga gccgctgtat gacgtcctgc gccagtatgt
      181 gctgatgccc ggtaaagtcc ataccgatga tatccccgtt ccggtccagg agccgggcag
      241 cggtaaaacc cggacagccc ggctgtgggt ctacgtccgt gatgaccgta acgccggttc
      301 acagatgccc ccggcggtct ggttcgcgta cagtccggac cggaaaggta tccatccaca
      361 aaatcacctg gccggttaca gcggtgtgct tcaggccgat gcttacggtg gttaccgggc
      421 gttatacgaa tccggcagaa taacggaagc cgcgtgtatg gctcatgccc ggagaaaaat
      481 ccacgatgtg catgcaagag cgcccaccta catcaccacg gaagccctgc agcgtatcgg
      541 tgaactgtat gccatcgagg cagaggtccg gggctgttca gcagaacagc gtctggcggc
      601 aagaaaagcc agagccgcgc cactgatgca gtcactgtat gactggatac agcaacagat
      661 gaaaacactg tcgcgtcact cagatacggc aaaagcgttc gcatacctgc tgaaacagtg
      721 ggatgcactg aacgtgtact gcagtaatgg ctgggtggaa atcggcaaca acatcgcaga
      781 gaacgcctta cggggagtgg ccgtaggccg gaaaaactgg atgttcgcgg gttccgacag
      841 cggtggtgaa catgcggcgg tgttgtactc gctgatcggc acatgccgtc tgaacaatgt
      901 ggagccagaa aagtggctgc gttacgtcat tgaacatatc caggactggc cggcaaaccg
      961 ggtacgcgat ctgttgccct ggaaagttga tctgagctct cagtaaatat caata
//
LOCUS       DAZFFE010000288         1012 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFFE010000288 DAZFFE010000000
VERSION     DAZFFE010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.291"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..198
                     /locus_tag="ACHJ69_004980"
     CDS             <1..198
                     /locus_tag="ACHJ69_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO5017748.1"
                     /translation="VAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELNRAI
                     NDAELVLGTTRRRRRPLGVRL"
     gene            195..>1012
                     /locus_tag="ACHJ69_004981"
     CDS             195..>1012
                     /locus_tag="ACHJ69_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5017749.1"
                     /translation="MKRKPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPSQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTESTQ
                     LFRTRFKAVSPKRVDTPGHGIGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELLTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKA"
BASE COUNT          206 a          236 c          343 g          227 t
ORIGIN      
        1 gtcgctgaac tgcaggcgct gcgtcaggcg cgccttgatt tattaaccgg taaacgggtg
       61 gtgtctgtcc agaaagatgg tcgcagaatt gaatatacgg cggcctctct ggatgagctt
      121 aaccgggcga tcaatgatgc tgagttggta ctggggacaa cccgccgtcg ccgtcgtccg
      181 ctgggagtga ggttatgaaa cgaaagcctg tcctgattga tgtgaacggc gttccgcttc
      241 gggagagtct cagctacaac gggggcggtg caggatttgg cgggcaaatg gcagagtggt
      301 tgccaccctc gcagagtgcc gatgcggccc tgctgcccgc gttgcgtctg gggaatgccc
      361 gtgcagatga tctggtgcgc aataacggaa tagcggccaa tgcggtggcc ctgcataagg
      421 atcacattgt cgggcatatg tttctgatta gctaccgtcc gaactggcgc tggctgggga
      481 tgcgggagac cgcggcaaaa agttttgtcg atgaggtgga ggcggcctgg tcagaatacg
      541 cagaagggat gtttggtgag atcgacgtgg aagggaaacg cacgtttacg gaatttatcc
      601 gtgaaggtgt gggcgttcat gcgtttaacg gcgaaatctt tgtgcagccg gtctgggata
      661 cggagagcac gcaactgttt cgtacgcgtt ttaaagccgt gagtccgaaa cgggtggaca
      721 cgccaggaca cggtatcggg aaccgttttc tgcgggccgg tgtggaggtt gatcgatatg
      781 gtcgtgccgt tgcgtaccat atctgtgagg atgattttcc tcgctccggg agtggacgat
      841 gggaacggat cccgcgtgaa cttctcaccg ggcgtccggc catgctgcat attttcgagc
      901 cggtggagga cgggcagacc cgtggggcca atcagtttta cagcgtcatg gaacggctga
      961 agatgctcga ttccctgcag gcaacacagc ttcagtcggc cattgttaaa gc
//
LOCUS       DAZFFE010000289         1006 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFFE010000289 DAZFFE010000000
VERSION     DAZFFE010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.292"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..75
                     /locus_tag="ACHJ69_004982"
     CDS             <1..75
                     /locus_tag="ACHJ69_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017750.1"
                     /translation="GGATWDIRKPYVDKVLVINTSMYD"
     gene            202..939
                     /locus_tag="ACHJ69_004983"
     CDS             202..939
                     /locus_tag="ACHJ69_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5017751.1"
                     /translation="MKSITKFLVCATLGLGGLTAQAHAASGDSTVSLGFAHLQLSGLN
                     NFVKDLSLHNLDTFNNFVNENYFNSPGEYDDAFVRGHDGKEKSPLGMNIKYRYEITDE
                     IGVITSFAWARSMTNAQAFIDVKPSDPSREVKKPAASARTDIRANYWSLLSGPSWRFN
                     EYLSVYSMAGVAVAKVTYDLKVNDNLNGGVGSFSESNSTKKTSIAWSVGAQFNFNESV
                     TMDVTYESSGSGDWKTDGLFAGIGLKF"
BASE COUNT          301 a          172 c          226 g          307 t
ORIGIN      
        1 ggtggtgcaa catgggatat tcgaaagcct tatgtagaca aagttcttgt tattaatact
       61 tcaatgtatg attagcacaa taaaatttat caatcatttt aagaaaaatt tttatttttt
      121 atttccaggc aggatggtaa ataatcaaaa atgtggaaag gacagagaga ataatgtttt
      181 taatatagga agattcttaa aatgaaaagt atcacaaaat ttcttgtgtg cgcaactctg
      241 ggcttaggtg gattaactgc acaggcacat gctgcatctg gtgatagtac cgtatccctt
      301 ggattcgctc accttcagct atccgggctg aataattttg ttaaggattt aagtcttcat
      361 aaccttgata cctttaacaa ttttgtgaat gaaaactatt tcaattcacc tggagaatat
      421 gacgatgcgt ttgtccgggg ccatgatggt aaagagaaaa gtcccttggg aatgaacatt
      481 aagtatcgtt atgagattac ggatgagatc ggtgttataa cctcttttgc atgggctcga
      541 tccatgacta atgcacaggc attcattgat gttaagcctt ccgatccatc acgagaggta
      601 aaaaaaccgg cagcttcagc gagaactgat atcagggcta attactggag tctactatcc
      661 ggtccttcgt ggcggtttaa tgaatacctc agtgtgtatt caatggcagg tgttgcggtt
      721 gcaaaagtaa cttatgattt gaaagttaac gacaatctta atggtggtgt aggtagtttt
      781 tctgaaagta acagcaccaa aaaaacgtct attgcatggt ctgtcggcgc gcaatttaac
      841 ttcaatgaaa gtgttactat ggacgtcact tatgaaagct caggctctgg cgactggaaa
      901 actgatggtt tatttgcagg cattggcctg aaattctgag taatacccga caacatgtca
      961 taacccgctg tgatggcggg ttttttgttg cccgtgcagg gcaaaa
//
LOCUS       DAZFFE010000290          999 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZFFE010000290 DAZFFE010000000
VERSION     DAZFFE010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.293"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..301)
                     /locus_tag="ACHJ69_004984"
     CDS             complement(<1..301)
                     /locus_tag="ACHJ69_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5017752.1"
                     /translation="MKGLLALLISSMVLPAHAGIVIYGTRIIYPAEHKEVMVQLMNQG
                     NRSSLMQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNK"
     gene            complement(382..924)
                     /locus_tag="ACHJ69_004985"
     CDS             complement(382..924)
                     /locus_tag="ACHJ69_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5017753.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDGAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTADGQYAIPFKAKYLKLT
                     DNSVNSGDVLSSLIMRVAQD"
BASE COUNT          286 a          217 c          187 g          309 t
ORIGIN      
        1 ctttattagt gggtagttta ttcggcataa ttttaatttt tacttgctgc ccggaatttg
       61 cccctatttt tgccactggt ggcgttaaca tgaaaggaac ctgaattttt tctggtggta
      121 atgacgtatc gccatcatca atccacgcct gcatcagcga agaacggttt ccctggttca
      181 tcaactgcac catcacttct ttgtgctctg ccgggtaaat aatacgcgtc ccgtagataa
      241 caataccggc atgtgcagga aggaccatag aagaaatgag taaagctaat aatcctttca
      301 tcttaatgcc tcttaaatta aaaggccgcc atggagatag cggccatgtt aattacctga
      361 agaatcttgc aattttattt attaatcctg cgccacacgc ataatcaggg aagataacac
      421 atcacctgaa ttcactgagt tatctgtcag tttcaggtat tttgctttaa acggaatggc
      481 atattgaccg tccgctgttt tataactctc gatgttgaaa ggtgcagaaa catccaggac
      541 atcgctgtta tcacctaaat agagataata acccacggta tcgttgctac tggctatcgc
      601 cttatcgttt ccggttattt tatcgttggt aatcaacgag agttttacac cagcaacttc
      661 tttcggacag ctaattttta acatgctgct ggttacaccg actgcaccat cataggtcga
      721 gtatttagct ttaccttcaa agaccgtagt tggtacagtt ggcatattaa tacttactga
      781 atcgccacct gcttcgaatt tacacggaga ttctgcaata ttccctttaa tggttaatgt
      841 tccggtatca acgtctgcgg caaatacccc agcgctcata aaaaaagaag aaaaaacagc
      901 ggcagcaata attgaacgtt tcataatatg ctccttaata aaatctattt ttggttgtta
      961 agagtctgta tctcttggaa acaaatttct cacaccaaa
//
LOCUS       DAZFFE010000291          999 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFFE010000291 DAZFFE010000000
VERSION     DAZFFE010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.294"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..188
                     /gene="bfd"
                     /locus_tag="ACHJ69_004986"
     CDS             <1..188
                     /gene="bfd"
                     /locus_tag="ACHJ69_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO5017754.1"
                     /translation="CLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVRAAR
                     EVMEDELMQLPEFKESA"
     gene            260..736
                     /gene="bfr"
                     /locus_tag="ACHJ69_004987"
     CDS             260..736
                     /gene="bfr"
                     /locus_tag="ACHJ69_004987"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO5017755.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREEG"
     gene            complement(765..>999)
                     /gene="gspO"
                     /locus_tag="ACHJ69_004988"
     CDS             complement(765..>999)
                     /gene="gspO"
                     /locus_tag="ACHJ69_004988"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin peptidase GspO"
                     /protein_id="HFO5017756.1"
                     /translation="CHKEGLGYGDFKLLAAAGAWCGWQTLPMILLIASLGGIGYAIVS
                     QLLQRRTITTIAFGPWLALGSMINLGYLAWISY"
BASE COUNT          279 a          210 c          261 g          249 t
ORIGIN      
        1 tttgtctttg taatggtatc agcgataaaa aaattcgtca ggctgtgcgc cagttttccc
       61 cccactcgtt ccagcaatta aaaaaattca ttccggtcgg caatcagtgc ggtaaatgtg
      121 tccgggccgc gcgtgaagtg atggaggatg aattaatgca actgccggag tttaaggagt
      181 ccgcataaac ggagtgtgtt ttttgactta ctcgtaagcc gttctacgct tcaaagagtg
      241 gaagcgaagg agtcaaaaaa tgaaaggtga tactaaagtt ataaattatc tcaacaaact
      301 gttgggaaat gagcttgtcg caatcaatca gtactttctt catgcccgga tgtttaaaaa
      361 ctggggtctc aaacgtctca atgatgtgga gtatcatgaa tccattgatg agatgaaaca
      421 cgccgatcgt tatattgagc gcattctttt tctggaaggt cttccaaact tacaggacct
      481 gggcaaactg aacattggtg aagatgttga ggaaatgctg cgttctgatc tggcacttga
      541 gctggatggc gcgaagaatt tgcgtgaggc aattggttat gccgatagcg ttcatgatta
      601 cgtcagccgc gatatgatga tagaaatttt gcgcgatgaa gaaggccata tcgactggct
      661 ggaaacggaa cttgatctga ttcagaagat gggactgcaa aattatctgc aagcacagat
      721 tcgcgaagaa ggttgagccg tgtcgggagg gagaaccctc ccggttaata agagatccag
      781 gccagatatc ccaggtttat catgctgccg agcgcaagcc acgggccgaa tgcgatagtg
      841 gttatagtcc ggcgttgcag aagttgtgaa acgatggcgt aaccaatgcc acccagcgag
      901 gcaatcagca gtatcattgg caacgtttgc cagccacacc acgcgcctgc agcggccagt
      961 agcttgaaat caccgtagcc caatccctct ttgtgacaa
//
LOCUS       DAZFFE010000292          993 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZFFE010000292 DAZFFE010000000
VERSION     DAZFFE010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.295"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(157..789)
                     /locus_tag="ACHJ69_004989"
     CDS             complement(157..789)
                     /locus_tag="ACHJ69_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO5017757.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVKQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          250 a          228 c          199 g          316 t
ORIGIN      
        1 ggattattgc ggtttacttt gaggaagata aaatgcgagt ttatagaacc gcgagcacca
       61 aataatttta tgaccacaac ctgtcgtatt gtggtcataa aaaattgtcg actggaggga
      121 gttaaatcct ttacctcaat atattgcctg tcaggtttag ttcacaaaca gtgcttcgct
      181 atcaacgatg attccatctt ccgcaccttt aagcgccagg tttgcctcgt agtattgctt
      241 tttatcaagc agtttctgcg catcagcaag tgcattacga gtctgtttaa gcggcatcaa
      301 atattggttt tccattacac caacaccggc caggcgaagc tcttccattg cccccttttt
      361 atcccctttc gccatttttt cgttggcaat ttttatcgcg gcttcttttt cgggtgtcgc
      421 tacataactc tcagaaattc cgacagatgc attaatgaca atatattggt catcattgac
      481 gttggtcttt ttacctggct tagcgaactt tgcccattcg gtactatcat ctgacagcaa
      541 agcggaagct tcattggtca gttcttttgc cttttccgga tcgccatgaa acagagccag
      601 gcgcgctacc tgcacgtcac gcatcgcata taaaccttgt tcagagatac gctctgctgc
      661 tgccgttttc tgggtttgct taacttgctg agtttgctgt tgtgccgcta ccggtgcatt
      721 atcagcagcc catacagttg aagatgccag aattgctgtg accatcgtag ccataattaa
      781 acgtttcata cttacctcat tctttaacag ttttagaaaa cgccgccatc atattgggtg
      841 agcatgaaat gagaaatcag agagcaacgg acattttgaa agggttatct tacaactgta
      901 gttattatcg caaagccgct tttagtctta cagttgtagg aatctgcttc ctgtcgcaga
      961 gaactattct tatataccac aaagatatgt cat
//
LOCUS       DAZFFE010000293          975 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFFE010000293 DAZFFE010000000
VERSION     DAZFFE010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.296"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(13..717)
                     /locus_tag="ACHJ69_004990"
     CDS             complement(13..717)
                     /locus_tag="ACHJ69_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5017758.1"
                     /translation="MKFNLSNLSAVLLASGMLMSTAVTAAPGDAAQFGGADTDWSTVD
                     YPRLTDMDDNVDSMGGKIRFTGRVVKATCKVATDSKQIEVVLPVVPSNLFTGIDVEAQ
                     GASNQTDFNINLTECSNTEDQKIEFRFTGTADSANKTLANEVEGSTDADNSGNAGATG
                     VGIRIYSKGTTNNGLINLNTTAAEGSASTAAYTIPGNATTHDFSAAFTAGYAQNGSTV
                     APGVVKSTASFVVLYE"
BASE COUNT          267 a          218 c          198 g          292 t
ORIGIN      
        1 tcgtatttat atttactcgt acagcaccac aaaacttgct gttgacttaa ctacaccagg
       61 tgcaacagtg ctaccgtttt gagcataacc tgcagtaaag gccgcgctga aatcatgggt
      121 cgtagcattt cctggaattg tataagcggc ggtggaggcg ctaccctctg ccgcagtagt
      181 attcaggtta atcagaccat tattcgtcgt acctttggag taaattcgaa tccctacacc
      241 agtcgccccc gcattgccgc tgttgtcagc atccgttgat ccttctactt cgttagcgag
      301 cgttttatta gcgctatctg cagtaccggt aaaacggaac tcaattttct gatcctctgt
      361 attgctacat tcggtcagat taatattgaa atcggtctgg ttgctcgccc cctgtgcttc
      421 tacgtcgata ccagtgaaaa ggttggaagg cacaaccggc aggacaactt caatctgttt
      481 tgaatcggtt gcgaccttac aggtagcttt cactacacgg ccagtaaagc ggattttccc
      541 ccccattgaa tcaacgttgt catccatatc agtgagtctg ggataatcaa cggtgctcca
      601 gtcagtatcc gccccaccaa attgtgctgc atcgccgggt gctgcggtta ccgcagtaga
      661 catcagcata cctgatgcca gtaatactgc ggataaatta gataaattga atttcattta
      721 tacctttcct tttgttaaaa aatccaagca acattggtgt ttttacattc tgcgttaata
      781 agttatatct cacaaaaaat aagctgacgt tggctaattc cagaaggttc tttagaagag
      841 taattgagtg aataatatta tatatgctgc ttagtaatgt acctaaaata atccaaagcc
      901 gcaataagtg agtttatact cacatataac acactgataa tgataaaatt tatttttacg
      961 tggggttgta atagg
//
LOCUS       DAZFFE010000294          973 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFFE010000294 DAZFFE010000000
VERSION     DAZFFE010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.297"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..534)
                     /locus_tag="ACHJ69_004991"
     CDS             complement(<1..534)
                     /locus_tag="ACHJ69_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO5017759.1"
                     /translation="MIYPEITGKSGEHLRLKTLEAVWIQGRLRMWGRWSYIGGGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGNQKSWLAHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIE"
     gene            complement(543..908)
                     /locus_tag="ACHJ69_004992"
     CDS             complement(543..908)
                     /locus_tag="ACHJ69_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5017760.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLIDDEQFDEINIVR
                     GQPVSGGRLGVKIYKIESE"
BASE COUNT          232 a          287 c          188 g          266 t
ORIGIN      
        1 ctctatacca aaagccataa ccattggttt gtacaaaata aattctgcca cctttagcca
       61 atgctcaatg cgtctttcac aggttcttaa actccattcc gggtgtgcat cattcagcag
      121 ttcagccatt ttgcgcttag tcatcccccg ccccacataa cgctgactca ggacattgag
      181 cagcccggga tagcctgcca ggacttcgcc aataaccctg tcgattatta acgcctctga
      241 atcggtacaa tgtgccagcc agcttttttg attgccgttg atcatatccc gcaaaaaagc
      301 ctcaagttca ggtttgtcca gaccagcttt tttcatcctc cggagcgcct cgttaattgc
      361 cgtttttgtc agctttttag aggtcagtaa ttggttgaac atatttcccg tcttaccgcc
      421 gccaatatac gaccaacgcc cccacatccg taatcgccct tggatccaga ctgcttccag
      481 cgtttttagg cgtaaatgct cgccgctttt gcctgtaatt tccggatata tcatatttac
      541 gctcactcac tctcaatttt gtaaatcttc acacccagcc gtccaccaga tactggctga
      601 ccacgtacaa tattgatttc atcaaactgc tcgtcatcaa tgagcactcc cgcatgcgtc
      661 agcgcatcca gcggtgcttt cagaatgttg tccaggtcgc gacgacgctt atccggtggc
      721 tctgcaatca cctttatcgc cagccttccg gacaggctta atttcagccg ctgctggcga
      781 acaataagcg ccactgcccg gcgataacgc tccccggctt ttgatacaaa atatgtgctg
      841 ccacggcgtc gccagtaagt gttcaccgtc ggcgggtaag gcaaaacaaa ttctatgcgt
      901 tcggtcattt atgctttcca cttcagaaca cccgaatttc tcgcgtgcat taaaaaacga
      961 atcagcaaca aca
//
LOCUS       DAZFFE010000295          973 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZFFE010000295 DAZFFE010000000
VERSION     DAZFFE010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.298"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>973
                     /locus_tag="ACHJ69_004993"
     CDS             <1..>973
                     /locus_tag="ACHJ69_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5017761.1"
                     /translation="VVVGVPVPDNIHPMTKDEAGVWSWRTPVLKGNLYEYFFNVDGVR
                     SIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVVHGDLIAITYHSNALQSERQMYVWTP
                     PGYTGIGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETD
                     AKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQ
                     GGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNEPAAINQQLRNFTVVVGD
                     KDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQK"
BASE COUNT          246 a          220 c          266 g          241 t
ORIGIN      
        1 gtagtggtgg gtgttccggt tccggacaat attcacccga tgaccaaaga cgaagcggga
       61 gtctggtcgt ggcgcacgcc tgtcctgaaa ggcaatctgt acgagtattt tttcaatgtt
      121 gatggtgtac gcagcattga tacaggcacg gcaatgacta agcctcagcg tcaggttaac
      181 tccagtatga ttctggtacc gggcagttat ctggatacac gttctgttgt gcatggtgat
      241 ttgatcgcca taacttacca ctccaacgca ttgcaatccg aacgtcagat gtatgtctgg
      301 accccgccag gatacaccgg cataggcgag cctttgccag tgctctattt ctatcacggc
      361 tttggtgata ccggacgttc cgctattgat caggggcgta tcccgcaaat catggataac
      421 ctgctggctg aagggaaaat taaaccgatg ctggtggtga tcccggatac cgaaaccgat
      481 gcgaagggca ttattcccga agatttcgtt cctcaggaga gacgtaaagt cttttatccg
      541 ttaaatgcta aagcggcaga tcgcgaactg atgaacgata ttatcccgct gattagcaag
      601 cgttttaatg tccgtaaaga tgccgatggt cgcgcactgg cagggctttc acaaggtggg
      661 tatcaggcac tggtatcggg aatgaatcat ctggaaagct ttggctggct ggccacattc
      721 agtggtgtta ccacgacgac cgtaccagat gaaggggtcg cagcccggct gaacgagccg
      781 gcagctatta accagcaact acgtaatttt actgtggttg ttggggataa agatgttgta
      841 accggcaagg atatcgccgg gctgaaaact gagcttgagc agaaaaaaat taagtttgat
      901 taccaggaat atccgggtct gaaccatgaa atggatgtct ggcggcctgc ctatgcagcc
      961 ttcgtacaga aat
//
LOCUS       DAZFFE010000296          965 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFFE010000296 DAZFFE010000000
VERSION     DAZFFE010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.299"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            344..909
                     /gene="ipuB"
                     /locus_tag="ACHJ69_004994"
                     /pseudo
     CDS             344..909
                     /gene="ipuB"
                     /locus_tag="ACHJ69_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224626.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpuB"
BASE COUNT          342 a          166 c          157 g          300 t
ORIGIN      
        1 caagtgttaa aactaacaca acaattaacc aaaataatat ggctgtttat ttttcatccg
       61 taaacaaaaa ggtacaaata ttttcaccac aaaaaaacca ccagcatggc attgaattac
      121 cattcacaat acacaccccc tttcgcttat ttatgctcat tggttgacta ataatgatct
      181 ataccccctg ggcaactatc tctctggcgg ggtattgatg aatatccatt tagtggcata
      241 atttaaaccc cagctcatta tttgcttata aaaacaagca ataataccag gctctggtat
      301 ttatagttta ttttttaact taattagtat aaccgataat attatgcgca aatttattac
      361 tcatagtgaa tggttgttat tttttgaagc catcaacggt tcaaaaaatg aaattagaga
      421 taaagccatg ttacaaatgg catatgtaca tggtcttagg gtaagcgagc ttattgcact
      481 aaagattagc gacattgact tttcagagtc agcaatatat atcaagcgtt taaaaaatgg
      541 attatccaca gtacatcccc tgcaaaaaga aactgttctg ttattaaaaa aatggctagc
      601 actacgtgat aacatagtaa aaaaaccttt tgaagactct ttctttcttg tcaaggaaat
      661 aaaatctcga gacaatatgt gtataagatg tgtaagaaat atagtcacaa tatgaatatt
      721 aatattcatc ctcacatgct taggcatggt tgtgggtatg ctttagctaa tcaagggtta
      781 gacaccagac taatacaaga ttatttaggg cacagaaata tacatcatac ggtattatat
      841 acagccagta atgcagcaag atttaaacga gtatgggagg gtgatgtgtt agatataaag
      901 aagatataac ccctgttcta aaggcctcaa ttcattgagg cttttagccc cttattttta
      961 tgaca
//
LOCUS       DAZFFE010000297          959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZFFE010000297 DAZFFE010000000
VERSION     DAZFFE010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.300"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..257
                     /locus_tag="ACHJ69_004995"
     CDS             <1..257
                     /locus_tag="ACHJ69_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017762.1"
                     /translation="NSNDSAVFASRCGRRFHAFKSDWFQHPPCTEEQAEWIIQCYRRR
                     GYEVKKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            259..>959
                     /locus_tag="ACHJ69_004996"
     CDS             259..>959
                     /locus_tag="ACHJ69_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5017763.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGGVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGYYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVCNQVADALPEAVLRRSLGLPAEKIRSLYRESDIIPGEQTATSILKQR
                     TKNIALPLHAHQQQIR"
BASE COUNT          223 a          270 c          256 g          210 t
ORIGIN      
        1 acaattctaa tgattctgcc gtctttgcca gcaggtgcgg acggcgtttt cacgcattca
       61 aatcagactg gttccagcat cctccatgca ctgaagagca ggctgaatgg ataattcagt
      121 gttaccgcag gcgtggatac gaggttaaga aagccctcag cctcgattat cgtcactgga
      181 taatctacgt caggctgccg tactccgagc gcccaccgcg tccgtcccgc acattccagc
      241 aacgcatctg gaggtaacgt gcgggtatta cttcgacctg ttctggtacc ggaactcggg
      301 ctggtgatcg ttaagccggg ccgtgaatcc atgccggtat tccacaatac ccgggtactg
      361 gtggagccgg aaccgaaaag catgcgtaat ctgccgtccg ggggcgttcc tgccgttcgc
      421 cagccgctgg cggaggataa atcattactg ccatttttca gcgacgaacg agtgattcgt
      481 gctgctggtg gcgctggcgc attgtctgac tggttactgc gccatgttaa atcctgccag
      541 tggccacacg gctattatca tcacagcgaa accgtcattc accgttatgg taccggcgca
      601 atggtgttgt gctggcactg cgacaaccag ctgcgtgacc agacatccga atcactcgag
      661 caacttgctc atcaaaacct gtcagcatgg atgattgacg tcatcggtca cgcaataagc
      721 ggtacgcagg agcgtgaatt atctctggct gaattatcct ggtgggcggt ctgcaatcag
      781 gtagcggacg cactaccgga ggctgtatta cgccgctcac tgggattacc ggcggagaaa
      841 atccgctcgc tttatcgcga gagcgacatc ataccgggag agcagacagc caccagcata
      901 ctgaagcagc gcacaaaaaa tattgcgcta ccacttcacg cccaccagca acaaatccg
//
LOCUS       DAZFFE010000298          936 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFFE010000298 DAZFFE010000000
VERSION     DAZFFE010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.301"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..506
                     /locus_tag="ACHJ69_004997"
     CDS             <1..506
                     /locus_tag="ACHJ69_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFO5017764.1"
                     /translation="YPTPFEDVNLNAINDLKKHFPKNNIGFSDHSSGFYAAIAAVPYG
                     ITFIEKHFTLDKSMSGPDHLASIEPDELKHLCIGVRCVEKSLGSNSKVVTASERKNKI
                     VARKSIIAKTEIKKGEVFSEKNITTKRPGNGISPMEWYNLLGKIAEQDFIPDELIIHS
                     EFKNQGE"
     gene            506..>936
                     /locus_tag="ACHJ69_004998"
     CDS             506..>936
                     /locus_tag="ACHJ69_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HFO5017765.1"
                     /translation="MRTKIIAIIPARSGSKGLRNKNALMLIDKPLLAYTIEAALQSEM
                     FEKVIVTTDSEQYGAIAESYGADFLLRPEELATDKASSFEFIKHALSIYTDYESFALL
                     QPTSPFRDSTHIIEAVKLYQTLEKYQCVVSVTRSNKPSQII"
BASE COUNT          335 a          141 c          180 g          280 t
ORIGIN      
        1 aatatccaac gccctttgag gatgtaaacc ttaatgctat taatgatttg aaaaaacact
       61 tccctaagaa taacataggc ttctctgatc attctagtgg gttttatgca gctattgcgg
      121 cggtgcctta tggaataact tttattgaaa aacatttcac tttagataaa tctatgtctg
      181 gcccagatca tttggcctca atagaacctg atgaactgaa acatctttgt attggggtca
      241 ggtgtgttga aaaatcttta ggttcaaata gtaaagtggt tacagcttca gaaaggaaga
      301 ataaaatcgt agcaagaaag tctattatag ctaaaacaga gataaaaaaa ggtgaggttt
      361 tttcagaaaa aaatataaca acaaaaagac ctggtaatgg tatcagtccg atggagtggt
      421 ataatttatt gggtaaaatt gcagagcaag actttattcc agatgaatta ataattcata
      481 gcgaattcaa aaatcagggg gaataatgag aacaaaaatt attgcgataa ttccagcccg
      541 tagtggatct aaagggttga gaaataaaaa tgctttgatg ctgatagata aacctcttct
      601 tgcttataca attgaagctg ccttgcagtc agaaatgttt gagaaagtaa ttgtgacaac
      661 tgactccgaa cagtatggag caatagcaga gtcatatggt gctgattttt tgctgagacc
      721 ggaagaacta gcaactgata aagcatcatc atttgaattt ataaaacatg cgttaagtat
      781 atatactgat tatgagagct ttgctttatt acaaccaact tcacccttta gagattcgac
      841 ccatattatt gaggctgtaa agttatatca aactttagaa aaataccaat gtgttgtttc
      901 tgttactaga agcaataagc catcacaaat aattag
//
LOCUS       DAZFFE010000299          916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZFFE010000299 DAZFFE010000000
VERSION     DAZFFE010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.302"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            54..839
                     /locus_tag="ACHJ69_004999"
     CDS             54..839
                     /locus_tag="ACHJ69_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017766.1"
                     /translation="MNLKKVCCICVLFSLLAGCASESPIDEKKKKTQTTQISINKNVP
                     QQLTDKDLYGNETTLAVSEEDIQAALEGDEFRVPLNSPVILVQSGSRAPETIMQEEMR
                     KYYTVATFSGIPDRKKTVTCNKDKNKDESLDIVDAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWTDYKNQKLTDTMSLRYLVRFTLVDVATGEWATWSPVNYE
                     SKVIFPQIGNKNSDSLDVAEQQISQLKQKTYAAVVKDLVNRYQ"
BASE COUNT          301 a          157 c          203 g          255 t
ORIGIN      
        1 aaatcgttag ctaaacatta ccatgattat gacaggcgag ggagtgtccg attatgaatt
       61 taaaaaaggt ttgttgtata tgtgtattat tctcgctgct tgcaggatgt gcctctgaat
      121 ctcctattga tgaaaaaaag aaaaaaactc aaaccacaca aatttctatt aataaaaacg
      181 ttccccagca actaacagac aaagatttat atggtaatga aaccacactg gctgtatccg
      241 aggaggatat tcaagctgcg cttgaaggag atgagtttcg tgtgcccctc aattctcctg
      301 taatacttgt tcagtctggc agtcgcgcac cggaaaccat tatgcaggag gagatgagaa
      361 aatattatac agttgccaca ttttctggca ttcctgacag gaaaaaaact gtaacctgta
      421 ataaagacaa aaacaaagat gaaagcctgg atattgtaga tgccgaaaat atgaactgga
      481 tgcaggcatt gcgttttgtt gcagcaaaag gccatcagaa agcgattatt gttttccagg
      541 atacgttgca gacaggaaaa tatgactctg gattgaaatc aatagtttgg acagattata
      601 aaaatcaaaa attgactgac acgatgtcac tgcgttattt ggttcgattc acgttggtgg
      661 atgtggctac aggtgagtgg gctacctggt cgcctgtgaa ttatgaatcg aaagtgattt
      721 ttccgcagat agggaataaa aattctgaca gccttgatgt ggcagaacag cagatttcac
      781 aacttaagca gaaaacgtat gcagcagtgg ttaaagacct ggtgaatcga tatcagtagt
      841 gaggttttat accagacgtt ttagtggtac ttagcaagaa agagcatttc tcttttctat
      901 tttacactat ggaaca
//
LOCUS       DAZFFE010000300          908 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZFFE010000300 DAZFFE010000000
VERSION     DAZFFE010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.303"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..482
                     /locus_tag="ACHJ69_005000"
     CDS             <1..482
                     /locus_tag="ACHJ69_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO5017767.1"
                     /translation="WVKADEIVANDYNPNVMAPSEKKLLKQSLEKDGFTQPIVVSEET
                     SHYLVVDGFHRQLLGRRTVTGKRLKGWLPVTCINPDRKGQTSRIAATIRHNRARGKHQ
                     ITSMSDIVRDLSRLGWTDERIGTELGMDQDEVLRLKQISGLTELFQQADFSPAWTVT"
     gene            complement(571..>908)
                     /locus_tag="ACHJ69_005001"
     CDS             complement(571..>908)
                     /locus_tag="ACHJ69_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017768.1"
                     /translation="SPWESWISDVADHKVEMWRTFSSVDELGELSPAEIESMDIIFGQ
                     YGGWDAFRLCDETHRICGEWRDPHGSSIPISLKDIFIALGKLPEAATVMANSINAQNN
                     LDILLGDLR"
BASE COUNT          280 a          208 c          193 g          227 t
ORIGIN      
        1 tgtgggtaaa agcggatgaa attgtcgcta acgattataa tcccaatgtc atggccccaa
       61 gcgaaaaaaa attactgaaa caatctctgg aaaaagacgg ttttactcaa ccaattgttg
      121 tttcggaaga aacaagccat tacctggtgg tggatggttt tcataggcag ttactaggta
      181 gaagaacagt tactggaaaa cgcctgaaag gctggctacc agtaacctgt attaatccag
      241 accgaaaggg acaaacctca cgtattgccg cgactatccg acataacaga gccaggggga
      301 aacaccagat aacctcaatg tctgatattg tcagagatct ttcacgtctt ggctggactg
      361 atgaacgtat cggaacagaa ctggggatgg accaggatga agttctgagg ttaaagcaga
      421 ttagcgggct aacggagtta tttcagcagg cagatttcag ccctgcatgg actgttacat
      481 aaagttaggt taggcctgtg gagccgggga atcggaatta aacatcttcc ctccccggta
      541 aatacaaata agggctgaac atcatcccga ctacctcaga tcacctaata atatatcaag
      601 attattttgc gcattaatgc tattcgccat tactgttgct gcttctggca actttccaag
      661 agcaataaat atatccttaa ggctgattgg aattgatgaa ccatgcggat cgcgccactc
      721 accgcaaatt ctgtgagttt catcacaaag cctgaacgca tcccaacctc catattgacc
      781 aaaaattatg tccattgact ctatttctgc gggcgataac tcccccagct catcaacaga
      841 agaaaatgta cgccacattt caaccttatg atccgctaca tcagatatcc atgattccca
      901 cggactgt
//
LOCUS       DAZFFE010000301          906 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFFE010000301 DAZFFE010000000
VERSION     DAZFFE010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.304"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>906
                     /locus_tag="ACHJ69_005002"
     CDS             <1..>906
                     /locus_tag="ACHJ69_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD1 domain-containing protein"
                     /protein_id="HFO5017769.1"
                     /translation="FKGVMKAINSSDMPRRSALLDEVRSKPYWSTDVEMAARAFERYV
                     QDKARTAGVENDYLVNIRKAPEHNTDNTWAYPTNAELDGGIRDAFDHLFSTLKTRETD
                     KGVAFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVARGVMSGIKDSDLKVRVV
                     KSQKEAEALAGESFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHEIIHHAME
                     HVVTPAEYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLAHMAEKQP
                     NKFVAAWERVVALVKGVLRRTGLLKP"
BASE COUNT          220 a          207 c          286 g          193 t
ORIGIN      
        1 tttaaaggcg tgatgaaagc gatcaacagc agtgacatgc cgcgtcgttc agcgcttctc
       61 gatgaggtgc gctcaaaacc gtactggtca acggatgttg aaatggcggc gcgtgccttt
      121 gagcgttatg ttcaggataa agcgcgtacg gctggcgtgg agaatgatta tctggtcaat
      181 atccgtaagg cacctgagca caacacagat aacacttggg cttatccgac gaatgcggaa
      241 ctggatggcg gtattcgtga tgcattcgat cacctgttca gtacgctgaa aacccgtgag
      301 acggacaagg gcgttgcgtt ttattcccgt aagggcgtga cccgcacacc tgaaggcaat
      361 ctcatttcgg atgttaaccg cagtgcggaa gccaaaggca gcccggtccc gcaggttgaa
      421 gcggttgccc gtggcgtgat gagcggcatt aaggacagtg acctgaaggt ccgtgtggtg
      481 aagtcacaga aagaggctga agcgctggcg ggggaatcat tcgacggtta cggcagggtg
      541 cacgcgttct atcgtccgga taaacgtgaa attgtcctgg tggcggataa catccctgac
      601 gggcggaccg ttcgcgagaa gctgcgtcac gagattatcc accatgcaat ggagcatgta
      661 gtcacgccag cggaatatca gacgattatt aagaccgtgc tgaaaacccg tgacagtgat
      721 aacgccacca tccgtgaagc ctggcgtaag gttgatgcgt cttatggtaa ggaatcaccg
      781 gaagtgcagg cgggtgaatt tctggcacat atggcggaga aacagccgaa taaatttgtg
      841 gcggcgtggg agcgtgttgt tgccctggtc aaaggggtac tgcgtcgcac ggggttactg
      901 aagccg
//
LOCUS       DAZFFE010000302          903 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZFFE010000302 DAZFFE010000000
VERSION     DAZFFE010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.305"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(42..638)
                     /gene="fimE"
                     /locus_tag="ACHJ69_005003"
     CDS             complement(42..638)
                     /gene="fimE"
                     /locus_tag="ACHJ69_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO5017770.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          289 a          201 c          163 g          250 t
ORIGIN      
        1 tgtatttatt tgttttttaa ctttattatc aataagttaa atcaaacttc ttctcttttt
       61 aatttttcgt ttatgagatt atttctttcc cataatccgg caaaacgagc agcattactg
      121 gcggtataac gcacagtatg gcgaatattt cgatgcccga gataatcctg aattaaacgg
      181 gtatctgcac cacgctccgc cagttcataa ccacaagcat gccttaacat atgaggatga
      241 gtctgcgtta cggttccagc ttcaataccg gcatcgcgaa taatgcgata ggcctgctgg
      301 cgagaaagcc gactcccgcg gcgagaaata aatatagcgt cggtccggtc agcgcctttc
      361 cagttagcac gttcctgggt ccagcgttcc acagcttcac gctcatcaaa acgtaacggg
      421 tgaacggtag aaaatccgtt cttcagtcgg cgaatattta ttcgaccttc attaaggtca
      481 agatcctgat aatgcagatc aagcagttca ctaatacgca tcccatgccg atatgccaac
      541 agaataagac aataatctct tgctcccgtt gccccgtaac acaccgcctg catcatggcc
      601 tgaacttctt taccggtaag ataacgacgt ttactcacaa tagtagtact cctgactgag
      661 atatatttga atgaatacct ataggaaacc tcaatcggtc aaaattagcc caatagataa
      721 aaagataacc acacacgaag tgatgtggct atcagtcaat tacattaaat atcatacaaa
      781 aataaaatat cactgatgtg acaacatcta ttttcattga tttgattcaa tataacaata
      841 gattgcagtg gattaacttc cagttgctta aaaaataaag aaaaaagtat gaaaacaggc
      901 gaa
//
LOCUS       DAZFFE010000303          899 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFFE010000303 DAZFFE010000000
VERSION     DAZFFE010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.306"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..113
                     /locus_tag="ACHJ69_005004"
     CDS             <1..113
                     /locus_tag="ACHJ69_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO5017771.1"
                     /translation="DISPSADVVDVSEDDVALMMASAGISGGVRYVPAGW"
     gene            94..>899
                     /locus_tag="ACHJ69_005005"
     CDS             94..>899
                     /locus_tag="ACHJ69_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO5017772.1"
                     /translation="MSQPVGDLVIDLSLDAARFDEQMSRVRRHFSGLDTDARKTAGAV
                     EQSLNRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALAYAWYQGDATLSEFNKTLVLSGNQA
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGA"
BASE COUNT          176 a          230 c          298 g          195 t
ORIGIN      
        1 gcgatatttc accttctgca gatgtggtgg atgtcagcga ggatgatgtt gcattaatga
       61 tggcttcagc ggggatttcc ggaggtgtga gatatgtccc agccggttgg tgatcttgtt
      121 attgacctga gtctggatgc ggcccgtttc gatgagcaga tgagccgggt aaggcgtcat
      181 ttttcaggac tggataccga cgccagaaaa accgccggtg ccgttgagca gagtctgaac
      241 cgtcaggcgc tggccgcaca aaaagccggg atttccgtcg ggcagtataa agcagccatg
      301 cgtaccctgc ccgcacagtt tacggatatc gccacgcagc ttgccggtgg tcagaatccc
      361 tggctgatcc tgctgcaaca gggtggtcag gtgaaggact ccttcggcgg gatgatcccc
      421 atgttcaggg ggcttgccgg tgcgatcacc ctgccgatgg tcggggtcac ctcgctggcg
      481 gtggcgacag gtgcgctggc gtacgcctgg taccagggcg acgccacgct ttcagaattt
      541 aataaaacgc tggtcctttc cggcaatcag gccggactga ctgccgatcg tatgctgacg
      601 ctctcaagag ccgggcaggc agcagggctg acgtttaacc aggcgagaga gtcactggca
      661 gccctggtga atgccggtgt gcgtggtggt gaacagtttg atgccattaa ccagagtgtc
      721 gcgcgttttg cgtctgcatc cggtgtggag gtagataaag tcgctgaagc cttcgggaag
      781 ctgaccactg acccgacgtc gggactgatg gcgatggcgc gccagttccg taacgtgacg
      841 gcagagcaga ttgcgtatgt tgcacaactg cagcgttccg gagacgaggc cggggcctt
//
LOCUS       DAZFFE010000304          899 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFFE010000304 DAZFFE010000000
VERSION     DAZFFE010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.307"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..113
                     /locus_tag="ACHJ69_005006"
     CDS             <1..113
                     /locus_tag="ACHJ69_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017773.1"
                     /translation="DILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            94..>899
                     /locus_tag="ACHJ69_005007"
     CDS             94..>899
                     /locus_tag="ACHJ69_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO5017774.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGA"
BASE COUNT          172 a          227 c          301 g          199 t
ORIGIN      
        1 gcgatatttt atcgtctgcg gatgtggcgg atgtcatggt ggatgatgcc gcattaatga
       61 tggcttcagc ggggattccg ggaggtgtga gatatgtccc agccggttgg tgatcttgtt
      121 attgacctga gtctggatgc tgtccgtttc gatgagcaga tgagccgggt aaggcgtcat
      181 ttttcaggtc tggataccga cgccagaaaa accgccagtg ctgttgaaca gggcctgagc
      241 cgccaggcgc tggctgcaca aaaagccggg atttccgtcg ggcagtataa agcggccatg
      301 cgaaccctgc cagcacagtt tacggatatc gccacgcagc ttgccggtgg tcagaatccc
      361 tggctgatcc tgctgcaaca gggcggtcag gtgaaggact ccttcggcgg gatgatcccc
      421 atgttcaggg ggctcgccgg tgcgatcacc ctgccgatgg tcggggtcac ctcgctggcg
      481 gtggcgacag gtgcgctggt gtacgcctgg taccagggag attccacgct ttcagcgttt
      541 aataaaaccc tggttctttc cggtaatcag tccggactga ctgccgatcg tatgctgacg
      601 ctctcaagag ccgggcaggc agcagggctg acgtttaacc aggcgagaga gtcactggca
      661 gccctggtga atgccggtgt gcgtggtggt gaacagtttg atgccatcaa ccagagtgtc
      721 gcgcgttttg cgtctgcatc cggtgtggag gtggataaag tcgctgaagc cttcgggaag
      781 ctgaccactg acccgacgtc gggactgatg gcgatggcgc gccagttccg taacgtgacg
      841 gcagagcaga ttgcgtatgt tgcacagctg cagcgttccg gagacgaggc cggggcatt
//
LOCUS       DAZFFE010000305          882 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZFFE010000305 DAZFFE010000000
VERSION     DAZFFE010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.308"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            340..>882
                     /gene="sitA"
                     /locus_tag="ACHJ69_005008"
     CDS             340..>882
                     /gene="sitA"
                     /locus_tag="ACHJ69_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFO5017775.1"
                     /translation="MRSIKKVTMLLGSLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQI"
BASE COUNT          249 a          201 c          179 g          253 t
ORIGIN      
        1 aattggagtg actgcaagtt ttctttattg attaatttat atttatcagg gtccacagta
       61 tttccttcat tattgaaggg atagatccat tgtccatgtg ctagcttgtt tcgtatttct
      121 atcaggattc gtagctcatt cgacaaaaca tcatgtaatg ctgctcttcg tgcggcatgt
      181 gctatcccaa gagtcccttc atccagcagg aaacaaacta atccctgttt tgggcctcaa
      241 aaatgctaat gataatcatt ttcattatgt ataggttatg ttataaaaca tagccgttgc
      301 tatgtttgtt ctaattttgc tcactatagg tactaaatta tgcgctcaat aaaaaaagta
      361 accatgctct tggggagcct cgcactcacc tgctcgatcg catttcaggc aagtgcaact
      421 gaaaaattta aggtcattac aacatttacc atcatcgcgg atatggccaa aaacgtggcg
      481 ggagatgctg cagaagtctc atccataacc aagcctggtg cagaaattca tgagtatcag
      541 cctacccctg gcgatattaa acgtgcgcag ggggcacaac tgattctcgc caatggtatg
      601 aatctggaat tgtggttcca acgcttttac cagcatctca atggggttcc agaagtaatt
      661 gtctcttcgg gtgtgacgcc tgtagggatc accgaaggac cctatgaggg caaacctaac
      721 ccccatgcat ggatgtcgcc agataatgct ctgatttacg tcgataatat tcgtgatgcg
      781 ttgataaaat acgatccggc aaatgcacaa acctaccaac gcaatgccga tacttataaa
      841 gccaagatta cccaaaccct tgcccccctg cgtaagcaga tt
//
LOCUS       DAZFFE010000306          876 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFFE010000306 DAZFFE010000000
VERSION     DAZFFE010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.309"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..510)
                     /locus_tag="ACHJ69_005009"
     CDS             complement(<1..510)
                     /locus_tag="ACHJ69_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO5017776.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPSGQRAHKPFIFTVALNKAVPLLYNALASGEMLPKVELHWWRTS
                     VEGKQEHYFTTRLTDATIVDMNLHMPHCQDPAQREFTQLLAVSLAYRKVEWEHIKSGT
                     SGADDWRAPL"
BASE COUNT          238 a          217 c          192 g          229 t
ORIGIN      
        1 cagcggtgca cgccagtcat cggcaccaga agtaccggat ttgatgtgct cccactcaac
       61 cttacggtag gccagtgata ctgcgagcaa ttgcgtaaac tcacgctgtg cagggtcctg
      121 gcagtgcggc atatgcaggt tcatgtccac aatcgtcgcg tcagtaagac gtgtggtgaa
      181 gtagtgttcc tgcttgcctt caacagaggt acgccaccag tgcagctcca ctttcggcag
      241 catttcaccg gaggccagcg cgttgtacag cagcggaacg gctttgttca gcgccacggt
      301 gaaaatgaac ggtttgtgag cacgctgccc ggaaggctga ccagactgcg gatcggttgg
      361 aacagtaacg ttatgcagaa actcctgcac cagcatttca tcttcgtgtc cctgcacgta
      421 gatattgccg acagaatctg cggtgaaagc accggcagtg atgttcccct gggtctgacc
      481 tgtaattgaa atgtaacatg gggttggcat ttatgaactc cttgtgaaat tatcaactgc
      541 tgttttacga acaacccgtc gttaatcccc gacaggttag agtgtgatgc ggctcacatc
      601 aggaaccaca cccttttctt tattcgagtt acaggcacac gtaattagca cctaccgaaa
      661 aactctttta tggtgtttaa atatattttt agtcaatttc agaacacgat cacataagct
      721 aaaaatatat caacatcaac agtagaatta tccataacgt atccttaccc cataaaaacc
      781 tacaatttct tcaaaaatca atcattacaa cacttacttc acaatcgttc cggcacgcaa
      841 ttaagatatt ccttgaagga atttcttatg atgtat
//
LOCUS       DAZFFE010000307          869 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFFE010000307 DAZFFE010000000
VERSION     DAZFFE010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.310"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..447
                     /locus_tag="ACHJ69_005010"
     CDS             <1..447
                     /locus_tag="ACHJ69_005010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017777.1"
                     /translation="GPAGAAGAKGAPGPKGDKGEPGATGPAGPQGKTGPAGPRGPAGP
                     QGAAGRNGNVSTEKYAVGSFLFGSISTLESESAIKENISGEKITEVAILYRVGVTYSP
                     TFSIVHNAGNSIPGTWRAFVSAFSLGAPGYDEQTMTTNLTLFQRIV"
     gene            461..>869
                     /locus_tag="ACHJ69_005011"
     CDS             461..>869
                     /locus_tag="ACHJ69_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO5017778.1"
                     /translation="MAIVVTDVRGAVYTRLGNISCEIKIGEQWFPFTASENDPENHGR
                     QLFTDLISGRYGEIKPYEEPPEALTQSRERKKVEINAWRNAMEAANYTFEHNGRKWDY
                     GKQTQARLEPSVAAAKAGILPEGFFWTDAENNDV"
BASE COUNT          234 a          186 c          262 g          187 t
ORIGIN      
        1 ggaccggctg gtgcagccgg agccaaaggt gccccggggc caaaagggga caaaggcgaa
       61 cccggagcaa caggtcccgc ggggccgcag gggaaaaccg gcccggcagg tcctcgtggt
      121 ccggcagggc cgcagggggc agcaggcagg aatggtaatg tatccacaga aaaatatgcg
      181 gttggctctt tcttatttgg ttcgatatcg actctcgaaa gtgaaagtgc aattaaagag
      241 aatatcagtg gcgaaaaaat tactgaagtg gcgattcttt accgcgttgg cgtcacgtat
      301 tcgcccacgt tctcgattgt tcataatgcc ggaaacagta tcccggggac atggcgggct
      361 tttgtgtctg ctttcagtct gggtgctccc ggatacgatg agcagaccat gacaacaaac
      421 ttaacgttat ttcaacgcat tgtgtaatac agggggaaga atggcgattg ttgtaacaga
      481 cgtcaggggg gcggtatata ctcgattggg taatatttcc tgtgagataa aaattggtga
      541 gcaatggttt ccttttactg cgtcagaaaa tgatccggaa aatcacggac gacaactttt
      601 tactgattta atttccggca ggtatggaga gattaagccg tatgaagaac cgccggaagc
      661 tttaacacag tcacgtgaac ggaagaaggt ggaaattaat gcctggcgta atgcgatgga
      721 agcggcgaac tacacctttg agcacaatgg caggaaatgg gactacggca aacagacaca
      781 ggcgcgtctt gagccgtcag tggctgccgc gaaagcagga atactgccgg agggcttttt
      841 ctggacggat gcggagaata acgacgtcc
//
LOCUS       DAZFFE010000308          838 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFFE010000308 DAZFFE010000000
VERSION     DAZFFE010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.311"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            296..>838
                     /gene="sitA"
                     /locus_tag="ACHJ69_005012"
     CDS             296..>838
                     /gene="sitA"
                     /locus_tag="ACHJ69_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFO5017779.1"
                     /translation="MLSIKKVTMLLGCLVLTCSIAFQASAAEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDAFIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQI"
BASE COUNT          235 a          205 c          160 g          238 t
ORIGIN      
        1 cttcatttag tgactgcccc tgggtttttc atgctctcct gcgtcctaga gaacttgcca
       61 ataacctaca ttatttttgc tttgccatta ccaatcgtcc tgaacaagtt cattttaaag
      121 ccaaagcgct gcccaacttt tatcctcaat accacatcgt cagcaggaaa taacctaatc
      181 cctgtgttgg gatcttataa tcgctaatga taattattta cattttatag cttctgctat
      241 aaaacatagt agttgctatg tttattctaa ttttgctcac tataggtact aaatcatgct
      301 ctcaataaaa aaagtaacca tgctcttggg gtgcctagtc ctcacctgct cgatagcatt
      361 tcaggcgagt gcagctgaaa aattcaaggt cattacaact tttaccatca tcgcagatat
      421 ggccaaaaac gtggctggag atgctgcaga agtctcatcc ataaccaagc ctggtgcaga
      481 aattcatgag tatcagccta cccctggcga tattaaacgt gcgcaggggg cacaactgat
      541 tctcgccaat ggtatgaatc tggaattgtg gttccaacgc ttttaccagc atctcaatgg
      601 ggttccagaa gtaattgtct cttcgggtgt gacgccagta gggatcaccg aagggcccta
      661 tgagggcaaa cctaaccccc atgcctggat gtcgccagat aatgctctga tttacgtcga
      721 taatattcgt gatgcgttta taaaatacga tccggcaaat gcacaaacct accaacgtaa
      781 tgccgatact tataaagcca agattaccca aacccttgcc cccctgcgta agcagatt
//
LOCUS       DAZFFE010000309          835 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFFE010000309 DAZFFE010000000
VERSION     DAZFFE010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.312"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(32..754)
                     /locus_tag="ACHJ69_005013"
     CDS             complement(32..754)
                     /locus_tag="ACHJ69_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO5017780.1"
                     /translation="MNELINSNAIKMTSIEIAELVGSRHDKVKQSIERLAVRGVIRNP
                     PMVVFEKINNLGLLRGVEAYVFEGEQGKRDSIIVVAQLSPEFTARLVDRWRELEGATA
                     KIPQTFSEALRLAADLEDQKAELEKQLALAAPKVEFADRVGEASGILIGNYAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMERGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
BASE COUNT          192 a          217 c          178 g          248 t
ORIGIN      
        1 attttgcatt tccttcgcgc tggtttagcc attaagcagc ctcccctgtt actttcagca
       61 ttccgttatc gagcagcttt ctggtcagcc actgttgacc acgcccggtg atttttgtgg
      121 tgaacgatat ctgtattccg tgatttgtgt tgaccgctgt ttctttcact gtgaaatagc
      181 cgcgctccat atattcctgc attggcacat ttcgccggga acctgaagcg ataaggattt
      241 tgtgatcgcg catccacgca aacagtttgt ttggaccaat tccaacaacc tttgcatagt
      301 ttccaatcaa aattccgctg gcctcgccaa cgcgatcggc aaactcaact ttaggtgctg
      361 cgagagcaag ctgtttctcc agttcagcct tctggtcttc aaggtcggcc gcaaggcgca
      421 atgcctcaga aaaggtttgt ggtattttcg cggttgcccc ttcgagttct cgccaacggt
      481 caacaagacg agcggtgaat tccggcgaca actgggcaac gacaataatg ctgtcgcgct
      541 taccttgttc gccctcaaaa acgtaagcct ctacgccacg aagtaatcct aagttattga
      601 ttttttcgaa aaccaccatt gggggatttc ggatcacacc tcgaaccgcc agtcgttcaa
      661 tggattgttt caccttgtca tgacgacttc ccaccaactc agcgatttca atgcttgtca
      721 ttttgatggc attgctattt atcagctcat tcattgtcat gtcctctcat attgaaaatt
      781 cagcaataaa aaacccagcc gaagctgggt ttgttaagtt gtcaattgtc agtag
//
LOCUS       DAZFFE010000310          826 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFFE010000310 DAZFFE010000000
VERSION     DAZFFE010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.313"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>826)
                     /locus_tag="ACHJ69_005014"
     CDS             complement(<1..>826)
                     /locus_tag="ACHJ69_005014"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO5017781.1"
                     /translation="LCNDTACSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHL
                     AQAGVDITLIYSGAHKVDGNQFEALPEEVRQDMQQRIDAARRMFAEKVAMYTGLSVDA
                     VTGTEAAVFEGQSGIEAGLADELINASDAISVMAAALNTHDTGGTMPQLTATEAAAQE
                     NQRVMGILTCQEAKGREHLATMLAGQPGMSVEQARAILTAAAPQQPVASTQSEADRIM
                     ACEEAKGREQLAATLAAMPEMTVEKARPILAAAPLADAGPSLRDQIMALDEAKGAEA"
BASE COUNT          149 a          277 c          216 g          184 t
ORIGIN      
        1 gcttctgccc cttttgcctc atccagggcc atgatctgat cacggagtga gggcccggca
       61 tccgccagtg gtgcagccgc caggatcggg cgggcttttt ccaccgtcat ctccggcatc
      121 gccgccagcg ttgccgccag ttgttcacga cctttagctt cttcacacgc cataatgcga
      181 tcggcttcac tctgcgtgga tgccaccggc tgctgtggtg ctgccgcggt cagaatcgcc
      241 cgggcctgtt caacgctcat acccggttgt cctgccagca tcgtggcaag gtgttcacgt
      301 cctttcgctt cctgacacgt caggatcccc atcactcgct ggttctcctg cgcggcagct
      361 tccgttgcag ttaattgcgg catagtgcct cctgtatcat gtgtgttcag cgccgcagcc
      421 atcacgctga tggcatccga cgcattgatt aattcatccg ccagcccggc ctcaatgccg
      481 gactgacctt caaaaacggc ggcctctgtt cccgtgactg catcaacaga cagaccggta
      541 tacatcgcca ctttttcggc aaacatccgg cgcgccgcat caatccgctg ctgcatgtcc
      601 tggcgaacct cttccggcaa cgcttcaaac tgattgccat ccaccttgtg cgcccctgag
      661 taaatcagcg tgatatccac accggcctgc gccagatgac cggcatagct gacatggctc
      721 atcatcacgc caatggagcc gatacgggat gtctgggtaa ccagccgtcg ggagcaggcc
      781 gacgccagca gcatggctgc agaacaggcc gtgtcattgc acagtg
//
LOCUS       DAZFFE010000311          810 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFFE010000311 DAZFFE010000000
VERSION     DAZFFE010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.314"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>810)
                     /gene="neuC"
                     /locus_tag="ACHJ69_005015"
     CDS             complement(<1..>810)
                     /gene="neuC"
                     /locus_tag="ACHJ69_005015"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFO5017782.1"
                     /translation="INTTSHTHILHSMSVCLNSFGDFFSNNTYDAVMVLGDRYEIFSV
                     AIAASMHNIPLIHIHGGEKTLANYDEFIRHSITKMSKLHLTSTEEYKKRVIQLGEKPG
                     SVFNIGSLGAENALSLHLPNKQELELKYGSLLKRYFVVVFHPETLSTQSVNDQIDELL
                     SAISFFKNTHDFIFIGSNADTGSDIIQRKVKYFCKEYKFRYLISIRSEDYLAMIKYSC
                     GLIGNSSSGLIEVPSLKVATINIGDRQKGRVRGASVIDVPVEKNAIVRGINI"
BASE COUNT          277 a          150 c          124 g          259 t
ORIGIN      
        1 tatatttatc cctctgacga ttgcattttt ttcaacgggt acatctatta cactggctcc
       61 acgaacacgg cctttctgcc tatcaccaat gttaattgtt gcaactttta aagatggaac
      121 ctcaattaaa ccagaggagg agttcccaat tagcccacaa gagtatttaa tcattgccaa
      181 ataatcttct gaacgaatag aaatcaaata tctgaactta tactctttgc aaaaatattt
      241 tacttttctc tgaattatat cagaaccagt gtcagcgtta ctgccaataa aaataaagtc
      301 gtgagtattt ttaaaaaaag aaatcgctga caataactca tctatttgat cattaaccga
      361 ctgcgtggaa agtgtttcag gatggaatac tacaacaaag taccgtttta acagtgaacc
      421 atattttagt tccaactcct gcttatttgg taaatgcaat gaaagagcat tttctgcacc
      481 aagagaacca atattaaaca cactaccagg cttttcacct agttgaatta ctcgtttttt
      541 atactcttct gtagaagtaa gatggagttt actcatttta gtaattgaat gcctaataaa
      601 ctcatcataa ttagctaatg tcttttcacc accatgaata tgaattaatg gaatattatg
      661 cattgatgct gcgatagcga ctgaaaatat ttcatatcta tcgcctaaaa ccataaccgc
      721 atcatatgtg ttatttgaaa aaaaatcacc aaacgaattg aggcaaacac tcattgaatg
      781 gagaatgtga gtatgtgaag ttgtattgat
//
LOCUS       DAZFFE010000312          804 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZFFE010000312 DAZFFE010000000
VERSION     DAZFFE010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.315"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            84..>804
                     /locus_tag="ACHJ69_005016"
     CDS             84..>804
                     /locus_tag="ACHJ69_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFO5017783.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILENNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKELPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIY"
BASE COUNT          290 a          103 c          148 g          263 t
ORIGIN      
        1 agaaaattaa aaaagcaatc agggatgtaa aagtcaaact aggattgaag agcaaataat
       61 aattatgatg aagggtaatt aaattggatt ttaaacatct tactcaattt aaagatataa
      121 ttgaactgga caagcgcccc gttaaacttg atgaacggga aacgtttaat gtctcatggg
      181 gtattgatga gaattaccaa gttggggctg cgatttcaat tgcttcaatt cttgaaaata
      241 ataagcaaaa caaatttacc tttcacataa tcgctgatta cctagacaaa gagtatattg
      301 aattattatc acaattagca acgaagtatc aaacagtaat taaattatat catattgatt
      361 ctgagccatt gaaggagcta cctcaatcaa atatctggcc agtatctatt tattatcgtt
      421 tgctttcatt tgattatttt tctgcgcgat tggattcatt attatatctt gatgctgata
      481 tcgtctgtaa ggggtcattg aacgagttaa tagcattaga gtttaaagat gaatatgggg
      541 ccgtggtaat tgatgtagat gccatgcaaa gtaaaagtgc tgagcgtttg tgtaatgagg
      601 attttaacgg tagctatttt aactctggtg taatgtatat taatttacgg gaatggttaa
      661 aacaaaggct aacggagaaa ttctttgatc tattatcaga tgaatcaatt ataaaaaaat
      721 taaagtatcc ggatcaagat attttaaact tgatgtttct acaccatgca aaaatattac
      781 cgagaaaata taattgtatt tata
//
LOCUS       DAZFFE010000313          799 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFFE010000313 DAZFFE010000000
VERSION     DAZFFE010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.316"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>799
                     /locus_tag="ACHJ69_005017"
     CDS             <1..>799
                     /locus_tag="ACHJ69_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017784.1"
                     /translation="VLSATVAGVLFGVSFGVAAEEIYTKGEYKVIVNGVSAIIYKNNA
                     PVIAGINTKTGSAITINQKELLDGSAKILTENGLNPKDPNVKLTADVKGKIQNYINTI
                     KYPVYAESLKKENLQNITVADIEKIKSNVDAVSTVITSKTAADYNQAVKNGMSSEAAL
                     TVAKDGGAVLKEFNRIDSDIDQLNKETTFALDADGNITLDENQGSGERYSVKQTVADI
                     KADTTVYQNKDGSYTLDQSAPGNVRVNDAVVSLDNRTRSNTQAIQNHS"
BASE COUNT          279 a          137 c          171 g          212 t
ORIGIN      
        1 ctgttttgtc tgcaactgta gcaggagttt tgtttggggt tagttttgga gttgcggctg
       61 aagaaattta tactaaaggt gagtataaag tgattgttaa tggtgtatca gctattattt
      121 ataagaataa tgctccagta attgcaggaa ttaatacaaa aacaggtagt gcgataacta
      181 ttaaccaaaa agagttattg gatggtagtg ctaaaatttt gacagagaac ggtttgaatc
      241 caaaggaccc aaatgtaaaa cttactgctg atgttaaagg aaaaatacaa aattacataa
      301 atacaataaa atatcctgtt tacgctgaat ccttgaagaa agaaaattta cagaatataa
      361 ccgtagctga tattgaaaag atcaaatcca atgttgacgc agtttcaaca gttattactt
      421 ccaaaaccgc agccgattat aaccaggctg taaaaaatgg tatgagttct gaagctgcac
      481 tgactgtagc taaagatggt ggtgcagtct taaaagaatt caaccgaatt gattcggata
      541 tcgaccaact gaataaagag acaacttttg cgcttgatgc cgatggcaat atcacgctgg
      601 atgaaaatca gggaagcggt gagcgttatt ctgtaaaaca aacagtcgca gatattaagg
      661 ctgacactac tgtctaccag aataaagatg gcagctatac acttgaccag agcgcaccgg
      721 gcaacgttcg tgtgaatgat gctgttgtca gcctcgacaa cagaacccgc agtaacaccc
      781 aggctatcca gaatcactc
//
LOCUS       DAZFFE010000314          790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFFE010000314 DAZFFE010000000
VERSION     DAZFFE010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.317"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>790
                     /locus_tag="ACHJ69_005018"
     CDS             <1..>790
                     /locus_tag="ACHJ69_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5017785.1"
                     /translation="AERESSQLKYTGEAQKAYERLQTPLDKYTARQKELNKALKDGKI
                     LQADYNTLMASAKKDYESTLKKPSGVKVSAGERQEDRAHAAMLALETELRTLEKHSGV
                     NEKISQQRRDLWEAENQYVVLKEAATKRQLSEQEKSLLAHEKETLEYKRQLADLGDKV
                     EHQKRLNELAQQAARFEQQQSAKQAAISAKARGLTDRQAQRESEEQRLREVYGDNPAA
                     LAKATSALKNTWSAEEQLRGSWMAGMKSGWGEWAESATDSFSQV"
BASE COUNT          215 a          188 c          271 g          116 t
ORIGIN      
        1 gggctgagcg tgagtcgtca cagctgaagt ataccggaga ggcgcagaag gcgtatgagc
       61 gcctgcagac gccgctggat aaatataccg cccgtcagaa agagctgaat aaggccctga
      121 aagacggaaa aatcctgcag gcggattaca acacgctgat ggcgtcggca aaaaaggatt
      181 atgaatcgac gctgaaaaag ccgtcaggtg tgaaggtgtc tgccggtgag cgccaggaag
      241 accgggcgca tgcagccatg ctggcgcttg aaaccgagct caggacgctg gaaaaacaca
      301 gcggtgtgaa tgagaaaatc agccagcagc gccgggattt atgggaagcg gaaaatcagt
      361 atgtggtcct gaaagaggcc gccacgaaac ggcagttatc tgagcaggaa aaatccctgc
      421 tggcccatga gaaagagacg ctggagtaca aacgccagct ggctgacctg ggcgacaagg
      481 ttgaacacca gaaacggctg aatgagctgg cacagcaggc ggcgcggttt gaacagcagc
      541 agagcgcgaa gcaggcggca atcagcgcaa aagcccgcgg actcaccgac cgtcaggcgc
      601 agcgggagtc ggaagagcag cgccttcgtg aggtgtacgg tgataatccg gctgcgctgg
      661 cgaaggccac atctgcactg aagaacacct ggtctgcgga ggagcagctt cgtggaagct
      721 ggatggccgg gatgaagtcc ggctggggcg agtgggcgga aagtgcgacg gacagttttt
      781 cgcaggttaa
//
LOCUS       DAZFFE010000315          785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFFE010000315 DAZFFE010000000
VERSION     DAZFFE010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.318"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..323
                     /locus_tag="ACHJ69_005019"
     CDS             <1..323
                     /locus_tag="ACHJ69_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO5017786.1"
                     /translation="MEAANYTFEHNGRKWDYGKSTQTRLEPSVAAVKAGKLPEQFFWT
                     DADNNDVPMDAETLIALSAAAEQAMFTKGLEIHVRQRTMKKEIEALGDAEAILAYKVG
                     MADR"
BASE COUNT          241 a          144 c          223 g          177 t
ORIGIN      
        1 cgatggaagc ggcgaactac acgtttgagc acaatgggcg taaatgggac tacgggaagt
       61 caacgcagac gcgtcttgag ccgtcggtgg cggcagtgaa agcggggaaa ctgccggagc
      121 agtttttctg gacggatgcg gacaataatg acgtaccgat ggacgctgaa acactgattg
      181 ctctgagtgc tgctgcagag caggcgatgt ttaccaaagg tctggaaatt catgtcagac
      241 agcgcacgat gaaaaaagag attgaagcgc tgggtgatgc ggaagctata ctggcgtata
      301 aggtcggcat ggctgacagg tgaaaaaaag aggggcgctc cagccccttg ctacaaagct
      361 atggctaaat aaagaatact caggtcgagg aaataatctg gcaatgataa ttaaaggaga
      421 tgcaacggcc tccttcaccc ctgtcaaaga taaaaaactg gggagtgcgt gctccccgag
      481 agcatgatac ttgtggttga tagttatttt tatccggcga caaagatatt gagatgaggt
      541 cgcggatgca atacagaatt ttcgtattgc atggtaactg ctgattcctg aaactgaaag
      601 tcgtcatgaa actgtaagcg aaaaaacggg gagacggcac gctccccaag gtcatacatg
      661 agatagtggt gtttttatag tcgaaagaat aattgcataa aaagagtgta aattaaacat
      721 tcaggggata cagaattttc gtaggctgag ggattgctga aaataattta gtgcgtcctt
      781 caatg
//
LOCUS       DAZFFE010000316          773 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFFE010000316 DAZFFE010000000
VERSION     DAZFFE010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.319"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            48..764
                     /locus_tag="ACHJ69_005020"
     CDS             48..764
                     /locus_tag="ACHJ69_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5017787.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          255 a          135 c          167 g          216 t
ORIGIN      
        1 taacgattta ccagtaatgt aaataaattt tcgaggagat cattccagtg ggacgtaaat
       61 gggccaatat tgttgctaaa aaaacggcta aagacggtgc aacgtctaaa atttatgcaa
      121 aattcggtgt agaaatctat gctgctgcta aacaaggtga acccgatcca gaattaaaca
      181 catctttaaa attcgttatt gaacgtgcaa agcaggcaca agttccaaag cacgttattg
      241 ataaagcaat tgataaagcc aaaggcggcg gagatgaaac gttcgtgcag ggccgttatg
      301 aaggctttgg tcctaatggc tcaatgatta tcgccgagac attgacttca aatgttaacc
      361 gtacgattgc taacgttcgc acaattttca ataaaaaagg cggcaatatc ggagcggcag
      421 gttctgtcag ctatatgttt gacaatacgg gtgtgattgt atttaaagga tcagaccctg
      481 accatatttt tgaaatttta cttgaagctg aagttgatgt tcgtgatgtg actgaagaag
      541 aaggtaacat tgttatttat accgaaccta ctgaccttca taaaggaatc gcggctctaa
      601 aagcagctgg aatcactgag ttctcaacaa cagaattaga aatgattgcg caatctgaag
      661 ttgagctttc cccagaagat ttagaaatct ttgaagggct tgttgatgcc cttgaagatg
      721 acgacgatgt acaaaaagtt tatcataacg tcgcaaatct ctaattatat ttt
//
LOCUS       DAZFFE010000317          772 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFFE010000317 DAZFFE010000000
VERSION     DAZFFE010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.320"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>772
                     /locus_tag="ACHJ69_005021"
     CDS             <1..>772
                     /locus_tag="ACHJ69_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO5017788.1"
                     /translation="PGQKGDTGERGPQGVQGPAGATGPSGPRGATGPQGAMGPRGETG
                     ARGEKGDPGGPQGPKGDTGPRGEPGPAGAKGERGETGPAGPKGEPGERGPQGLQGPAG
                     ERGEPGPAGPKGERGEAGPQGPRGEPGPAGSAANVADATTAQKGIVQLSSATDSDDET
                     KAATPKAVKAAMDEARAAKQKAEEVATTRPSGESIPGPKGDRGEQGPAGPTGPKGERG
                     EPGPQGPAGQKGEQGLRGLQGATGPAGAVGPAGPRGPAG"
BASE COUNT          193 a          195 c          318 g           66 t
ORIGIN      
        1 taccgggcca gaaaggtgat acaggagaac ggggacctca gggagtacag gggccagctg
       61 gcgcaacagg gccatctggc ccacgtggtg cgaccgggcc acagggagca atggggccgc
      121 gtggtgaaac cggggccaga ggcgaaaaag gagacccggg ggggcctcag ggaccaaagg
      181 gggatactgg tccgagaggt gagccggggc cggcaggagc aaagggagag cgcggtgaga
      241 ccggacctgc ggggccgaaa ggggaacccg gggaacgtgg tccgcaagga ttacagggcc
      301 ccgccggaga gagaggtgaa ccgggtccgg caggtccgaa aggtgaacga ggcgaagccg
      361 gacctcaggg acctcgtggg gagccaggcc cggcgggcag cgcagcaaat gtggcagatg
      421 cgacgacggc acagaaggga attgtgcagt taagcagcgc aacggacagt gatgatgaaa
      481 cgaaggcagc cacaccgaaa gctgtgaagg cggctatgga tgaggccagg gcagcgaaac
      541 agaaggcaga agaggttgcg acaacccgcc cgtcgggaga aagcatacct ggaccgaaag
      601 gtgacagggg cgagcaagga ccggcaggtc ccacaggtcc gaaaggtgag cgcggtgaac
      661 ccggacctca gggacctgca ggacagaaag gtgagcaggg cctccgtggg ctgcaggggg
      721 cgacaggacc tgcgggagca gtggggcccg caggcccacg gggaccggct gg
//
LOCUS       DAZFFE010000318          770 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFFE010000318 DAZFFE010000000
VERSION     DAZFFE010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.321"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>770
                     /locus_tag="ACHJ69_005022"
     rRNA            <1..>770
                     /locus_tag="ACHJ69_005022"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          217 a          167 c          236 g          150 t
ORIGIN      
        1 gggacacgtg gtatcctgtc tgaatatggg gggaccatcc tccaaggcta aatactcctg
       61 actgaccgat agtgaaccag taccgtgagg gaaaggcgaa aagaaccccg gcgaggggag
      121 tgaaaaagaa cctgaaaccg tgtacgtaca agcagtggga gcctcttaat ggggtgactg
      181 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      241 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      301 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      361 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      421 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      481 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
      541 accggagaat gttatcacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
      601 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
      661 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
      721 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg
//
LOCUS       DAZFFE010000319          770 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFFE010000319 DAZFFE010000000
VERSION     DAZFFE010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.322"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>770
                     /locus_tag="ACHJ69_005023"
     CDS             <1..>770
                     /locus_tag="ACHJ69_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO5017789.1"
                     /translation="ENQLDTETITVSYEFILCQHLITNTEFSYLALPENYNRLFLPNS
                     KNKTNNGLKTLNSEAVGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEKTAG
                     IAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIYIKRPSKDLANLR
                     KEFNKNVRKNFLIKLAKTSEASGRFNAEDLLRMRKGNVPLNYNVHHKLSLDDGGTNDL
                     ENLVLIENEPYHKVFTNMQSRIAKGILVGESKITPWAI"
BASE COUNT          300 a          131 c          135 g          204 t
ORIGIN      
        1 tgaaaaccaa ctggatacag aaacaataac tgttagttat gaatttatcc tctgccaaca
       61 tcttatcact aacaccgagt tcagctattt ggcactccct gaaaattata accgcttgtt
      121 tttaccaaat tcaaaaaaca aaacaaataa tggtttaaaa acgttaaaca gcgaagctgt
      181 tggcaggcta cttgctgctg gtggcgtata caatgggaac attgaaggat tcagagatac
      241 tgcggaaaaa ctgggtggag atgcaatcaa aggctatgat caaatactaa atgaaaaaac
      301 agcgggcata gcgatagcaa cagcatctat tcttttaaca aagcgttcta atgttgatac
      361 atatacagaa ataaatagtt acttaggcaa acttagaggt caacaaaaac ttcttgatgg
      421 tatagacata atagaaataa tatacattaa gagaccttca aaagacttag ctaacttacg
      481 aaaggagttt aataaaaatg taagaaaaaa ttttcttatc aaacttgcaa aaacctccga
      541 agcatctgga agattcaacg ccgaagacct tttaagaatg agaaagggca atgttcctct
      601 aaattataat gtacaccata aactatctct agatgatggt ggtactaatg atttggaaaa
      661 tttagtatta atcgaaaacg aaccatatca taaagttttt actaacatgc aatcacgaat
      721 agctaaggga atattagtgg gtgaaagcaa aatcactccc tgggccattc
//
LOCUS       DAZFFE010000320          768 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFFE010000320 DAZFFE010000000
VERSION     DAZFFE010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.323"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..337
                     /locus_tag="ACHJ69_005024"
     CDS             <1..337
                     /locus_tag="ACHJ69_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO5017790.1"
                     /translation="PNIILGDGIFIGKMCILNRDTRIHDAVVINTRSLIEHGNEIGCC
                     SNISTNVVLNGDVSVGEETFVGSCTVVNGQLKLGSKSIIGSGSVVIRNIPSNVVVAGT
                     PTRLIRGNE"
     gene            334..>768
                     /locus_tag="ACHJ69_005025"
     CDS             334..>768
                     /locus_tag="ACHJ69_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFO5017791.1"
                     /translation="MSNIYIVAEIGCNHNGSVDIAREMILKAKEAGVNAVKFQTFKAD
                     KLISAIAPKAEYQIKNTGELESQLEMTKKLEMKYDDYLHLMEYAVSLNLDVFSTPFDE
                     DSIDFLASLKQKIWKIPSGELLNLPYLEKIAKLPIPDKKIIIS"
BASE COUNT          269 a          101 c          154 g          244 t
ORIGIN      
        1 accaaacatt atactgggtg atggaatttt tattggtaaa atgtgtatac ttaaccgtga
       61 tactagaata catgatgccg ttgtaataaa tactaggagt ttaattgaac atggtaatga
      121 aataggctgc tgtagcaata tctctactaa tgttgtactt aatggtgatg tttctgttgg
      181 agaagaaact tttgttggta gctgtactgt tgtaaatggc cagttgaagc taggctcaaa
      241 gagtattatt ggttctgggt cggttgtaat tagaaatata ccaagtaatg ttgtagttgc
      301 tgggactcca acaagattaa ttagggggaa tgaatgagta atatatatat cgttgctgaa
      361 attggttgca accataatgg tagtgttgat attgcaagag aaatgatatt aaaagccaaa
      421 gaggccggtg ttaatgcagt aaaattccaa acatttaaag ctgataaatt aatttcagct
      481 attgcaccta aggcagagta tcaaataaaa aacacaggag aattagaatc tcagttagaa
      541 atgacaaaaa agcttgaaat gaagtatgac gattatctcc atctaatgga atatgcagtc
      601 agtttaaatt tagatgtttt ttctacccct tttgacgaag actctattga ttttttagca
      661 tctttgaaac aaaaaatatg gaaaatccct tcaggtgagt tattgaattt accgtatctt
      721 gaaaaaatag ccaagcttcc gatccctgat aagaaaataa tcatatca
//
LOCUS       DAZFFE010000321          747 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFFE010000321 DAZFFE010000000
VERSION     DAZFFE010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.324"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>747
                     /locus_tag="ACHJ69_005026"
     CDS             <1..>747
                     /locus_tag="ACHJ69_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO5017792.1"
                     /translation="YGDPVDPVDPGWSGFYWIRFVNAAGVKGPWNAVKGTPAQTQISV
                     QAIIDQIKEEAAKSPVVEELRKEIKNAQGQAVKDAAIETTEVVGNLREETLKTIGGID
                     TRITGMNKSTSEELNKVNERITKLDKEGGEAFLAMWSKKAGVDGITAGIGIVAGKDSE
                     GRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETLVSRKVVA
                     DEVKAGVSITSPVIRSAVIQNGNFQVDSQGN"
BASE COUNT          206 a          146 c          230 g          165 t
ORIGIN      
        1 tacggtgatc cggttgatcc ggttgatccg ggctggtcgg gattctactg gatacgcttt
       61 gttaatgcag cgggagtgaa agggccatgg aacgcggtaa aaggcactcc cgcacaaacc
      121 cagataagcg ttcaggccat tattgaccag atcaaggagg aggctgcaaa gtcaccggtg
      181 gtggaagagc tgcgtaagga aataaagaat gctcaggggc aggcagtaaa ggacgcggca
      241 atcgagacga cggaagttgt gggtaatctc agggaagaaa cactgaaaac tatcggtggt
      301 attgataccc gtattacggg gatgaataag tcaaccagtg aagagcttaa taaggtaaat
      361 gagcgcatca ctaaactgga taaagaaggc ggtgaggcct tccttgcgat gtggtcaaaa
      421 aaagcgggag ttgatggcat cactgcgggg atcgggattg tcgccggaaa agacagtgaa
      481 ggcaggcctg taagtcaggt tgcaatttct gcgtcacagc tgtttgtctt tgacccgaat
      541 aatccggata acaccgccta tccgtttgcg gtctctggtg gaaaagttgt catccccaaa
      601 gcgatgattt atgacgcggt gattgaaaca ctggtgtcgc ggaaggttgt ggcggatgag
      661 gtaaaagccg gggtaagtat cacttcgcca gttatccgga gtgccgttat tcagaacgga
      721 aactttcagg ttgattctca gggtaac
//
LOCUS       DAZFFE010000322          740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFFE010000322 DAZFFE010000000
VERSION     DAZFFE010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.325"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..377)
                     /locus_tag="ACHJ69_005027"
     CDS             complement(<1..377)
                     /locus_tag="ACHJ69_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO5017793.1"
                     /translation="MDMQKETIFSEVETANSKQIAVLKANFPQCFDKNGAFIQEKLLE
                     IVKSSDVELSKESYSLNWLGKSYARLLANLPPKTLLAEDKDHNQREENKNSQNLLIKG
                     DNLEVLKHMVNAYAEKVKMIYIDP"
     gene            complement(389..>740)
                     /locus_tag="ACHJ69_005028"
     CDS             complement(389..>740)
                     /locus_tag="ACHJ69_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017794.1"
                     /translation="PFYYAFSAIFDIVAVLNTEEIIMISGLNGGNFILSSMAKERVKS
                     GLLDRATIEGSKGEYTVIFDGSEWEGKPLVLSSSRQPYEARVFKSIDGAAAELLRIGL
                     SEASIKNTTKSNNK"
BASE COUNT          200 a          160 c          125 g          255 t
ORIGIN      
        1 ggatcaatat aaatcatctt caccttttca gcataagcat taaccatatg tttcaatact
       61 tcgagattat cccctttgat caatagattc tgactgttct tgttctcttc acgttggtta
      121 tggtctttat cttcggcaag caacgtcttc ggtggtaagt tagccaacag ccgcgcatag
      181 gacttaccca gccagtttaa gctgtatgac tctttagaga gttcaacatc cgatgatttg
      241 acgatctcaa gcaacttttc ttgaatgaaa gccccgtttt tatcaaaaca ctgcggaaag
      301 ttagccttta aaacagcaat ctgcttactg ttagcagttt caacttctga aaaaatcgtt
      361 tctttttgca tatccatttt aaacctgatt atttattgtt cgatttggtg gtgtttttga
      421 ttgaagcttc gcttaagcct atgcgtagta gttctgcagc tgcaccatct atgcttttga
      481 atactcgtgc ttcataaggc tgtctggagc tacttaagac taatggctta ccttcccact
      541 ctgaaccatc aaaaatcacg gtatactcgc ctttagaacc ttctattgtt gctctatcaa
      601 gtaaacctga tttgactctt tccttagcca tcgagctaag gatgaaattt ccaccattaa
      661 gacctgatat catgattatt tcctcggtat ttaatactgc tacgatatca aatatcgctg
      721 aaaaggcata atagaatggt
//
LOCUS       DAZFFE010000323          738 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFFE010000323 DAZFFE010000000
VERSION     DAZFFE010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.326"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>738
                     /locus_tag="ACHJ69_005029"
     CDS             <1..>738
                     /locus_tag="ACHJ69_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO5017795.1"
                     /translation="YGDPVDPGWSGFYWIRFVNAAGVKGPWHAVAGVAAQTQISVQAV
                     IDQIKEEAAKSPVIEELRKEIKNAQGQAVKDAAIKTTEVVGTLREETTRTIGGIETRI
                     STLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGIVAGKDSEGRP
                     VSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYNAVIETLVSRKVVADEV
                     KAGVSITSPVIRSAVIQNGNFQVDSQGN"
BASE COUNT          202 a          151 c          228 g          157 t
ORIGIN      
        1 tacggcgatc cggttgatcc gggatggtcg ggattttact ggatcaggtt tgtgaatgcc
       61 gcaggcgtta aagggccgtg gcacgcagtg gcaggcgtgg cagcacagac acagatatca
      121 gttcaggcgg tgattgacca gataaaagaa gaggcggcga agtctcccgt catagaagag
      181 cttcgcaagg aaataaaaaa cgcgcagggg caggctgtaa aggatgctgc aattaagaca
      241 accgaagttg tggggactct cagggaagaa acgacaagaa cgattggtgg tattgaaacc
      301 cgcattagca cactggattc gtcaaccagt gaatcgctta atgaggtcga caagcgcatc
      361 actaaactgg ataaagaagg cggtgaggcc ttccttgcga tgtggtcaaa aaaagcggga
      421 gttgatggca tcactgcggg gatcgggatt gtcgccggaa aagacagtga aggcaggcct
      481 gtaagtcagg ttgcaatttc tgcgtcgcag ctgtttgtct ttgacccgaa caacccggat
      541 aacaccgcct atccgtttgc ggtatcaggt ggcaaggtcg tgatcccgaa agcgatgatt
      601 tataacgcgg tgattgaaac actggtgtcg cggaaggttg tggcagatga agtaaaagcc
      661 ggggtaagta tcacttcgcc agttatccgg agtgccgtta ttcagaacgg aaactttcag
      721 gttgattctc agggtaac
//
LOCUS       DAZFFE010000324          704 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFFE010000324 DAZFFE010000000
VERSION     DAZFFE010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.327"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..446)
                     /locus_tag="ACHJ69_005030"
     CDS             complement(<1..446)
                     /locus_tag="ACHJ69_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017796.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDDRVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SEIVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAHQNLSAWMIDV"
     gene            complement(448..>704)
                     /locus_tag="ACHJ69_005031"
     CDS             complement(448..>704)
                     /locus_tag="ACHJ69_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017797.1"
                     /translation="NSDNSAVFASRHGRRSHAFKSDWLQHAPCTEEQAEWLIQNYRRR
                     GYEFRKTLSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
BASE COUNT          169 a          188 c          195 g          152 t
ORIGIN      
        1 atgacgtcaa tcatccatgc tgacaggttt tgatgagcaa gttgcccgag tgattcggat
       61 gtctggtcac gcagctggtt gtcgcagtgc cagcacaaca ccatcgcgcc ggtaccgtaa
      121 cgatgtatga cgatttcact gtgatgatag tcaccatgag gccactggca ggatttgaca
      181 tgacgcaaca gccagtcaga cagtgcccca gcaccaccag cagcacgaat cacccgatca
      241 tcgctgaaaa atggcagtaa tgatttatcc tccgccagcg gctggcgaac agcagggacg
      301 actccggacg gcagattacg catgcttttc ggttccggct ccaccagtac ccgggtattg
      361 tggaataccg gcatggattc acggcccggc ttaacgatca ccagcccgag ttccggtacc
      421 agaacaggtc gaagtaatac ccgcacgtta cctccagatg cgttgctgga atgtgcggga
      481 cggacgcggt gggcgttcag agtaaggaag cctgacggag attatccagt gacgataatc
      541 gaggctgagg gttttcctaa actcataccc acgtctgcgg tagttctgaa tcagccattc
      601 ggcctgttct tcagtgcatg gggcgtgctg taaccagtca gatttgaatg catgagaacg
      661 ccgcccgtgc ctgctggcaa agacggctga attatcagaa ttgt
//
LOCUS       DAZFFE010000325          703 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFFE010000325 DAZFFE010000000
VERSION     DAZFFE010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.328"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>703
                     /locus_tag="ACHJ69_005032"
     CDS             <1..>703
                     /locus_tag="ACHJ69_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HFO5017798.1"
                     /translation="EKYALIEQWRQQFPIEAMCQVFGVSRSGYYNWVQHEPSDRKQSD
                     ERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRA
                     TTNPNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGY
                     AMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSR
                     KGNCYDNAPMESFWGT"
BASE COUNT          198 a          172 c          191 g          142 t
ORIGIN      
        1 tgaaaaatac gcgttaatcg aacaatggcg acaacaattt cccattgaag cgatgtgtca
       61 ggtatttggt gtatccagga gcggttatta caactgggta cagcatgaac cctcagacag
      121 aaaacaaagt gatgagcggc taaaactgga gattaaggtg gcacatatcc gcactcgcga
      181 aacatatgga acccggcggc tccagacgga gctggcagag aatggcatca tcgttggtcg
      241 tgaccgactg gcacgtcttc gtaaggagct aaggctacgc tgtaagcaga aacgcaagtt
      301 cagagcgact acgaacccga accacaatct gccagttgcg ccaaatctgc tgaaccagac
      361 gttcgctcct acagcaccaa atcaggtctg ggtggcggac ctgacgtatg ttgccacaca
      421 ggagggatgg ttgtacctcg ctggcatcaa agatgtttat acgtgcgaaa ttgtcggcta
      481 cgccatggga gagcgcatga caaaagagct gacaggtaaa gccctgttta tggcgctcag
      541 gagccagcgc ccacctgccg ggctaatcca ccactctgat cgaggttcac agtactgcgc
      601 atacgattac cgggtcatac aggagcagtt tggtctgaaa acatcaatgt cgcgtaaagg
      661 taactgttac gacaacgctc cgatggaaag cttctgggga acg
//
LOCUS       DAZFFE010000326          701 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFFE010000326 DAZFFE010000000
VERSION     DAZFFE010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.329"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..181
                     /locus_tag="ACHJ69_005033"
     CDS             <1..181
                     /locus_tag="ACHJ69_005033"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017799.1"
                     /translation="NNGNNAPTLKAPGLTEGTSEKSAQTTDIGLLFCTEFSEKLLLPL
                     PDGCGEDDEEKRPQL"
     gene            complement(229..651)
                     /locus_tag="ACHJ69_005034"
     CDS             complement(229..651)
                     /locus_tag="ACHJ69_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017800.1"
                     /translation="MTGNMTDNFKYKISAGFKPGLMMSGFFVHCIITNEEQLDPKSAY
                     FDAREKPKYAIVLAYPYEDRKLKIRDTAYEKFSCTEDEYKHFNSCSDLHGKEVYICVD
                     VNSWAVSAERSGVWYRYQSGTMKRFDGLPLDAPSGKEK"
BASE COUNT          243 a          154 c          118 g          186 t
ORIGIN      
        1 aaataatggc aataatgctc ctacgctaaa agccccggga ttaaccgaag gcacatcaga
       61 aaaatccgca caaacaacag atataggctt attattttgt acagaatttt cagaaaaatt
      121 actgttaccg ttaccagatg gctgtggtga agatgacgaa gaaaaaagac cacagttata
      181 agaaccatca ggctgaacct gacaaaccat aaaactcaac cctcagtatt acttttcctt
      241 acctgatgga gcatcaagag gcaaaccatc aaaccgcttc atcgttccgg actgataacg
      301 ataccagaca ccggaacgtt cagctgaaac cgcccaggaa ttcacatcaa cacagatata
      361 aacttccttc ccgtggaggt cagaacagga attgaaatgt ttatattcat cttctgtaca
      421 tgaaaatttt tcataagcgg tatcccgaat ttttaatttg cggtcctcat aaggatacgc
      481 cagaacaatg gcatatttag gtttttccct ggcatcaaag taagcacttt tagggtcaag
      541 ctgctcttca ttagtaatga tacagtgaac aaaaaaacca ctcatcatca gacctggttt
      601 aaaaccggca gatattttat atttaaaatt atcagtcata ttcccagtca tattttaatc
      661 ctcatgtatc aacaggaaag ttattgtcac aagtccaagt c
//
LOCUS       DAZFFE010000327          701 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZFFE010000327 DAZFFE010000000
VERSION     DAZFFE010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.330"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          259 a          155 c          100 g          187 t
ORIGIN      
        1 aacattcagg gtaagatgat gaaattcaca tgaaaaatgc tgaagattat ttatttcatg
       61 cagataaacg ataaaaaaca agagagtata tttttcagtg taagagtcaa ctgttcatct
      121 ccactgacaa tcacggtcag catacaaaca aagtgagtcc tcctgcttag tcattgcgta
      181 tcagaaacta aaaaacaaaa gattctgcaa acaacgtgac atttttatga catttcccga
      241 atacatgtat atcaataaac ggtagtcatc gttgaattaa taagtcttac agagctatac
      301 gtagcttata aaaccactca acgaccatca tcacctgact atttagcggg agtatcgtct
      361 gtataaatcc ataaataata acacctgtta ttaaaataag aatgctgtca gccgtaaact
      421 attaacacaa cacctgtcca ctctatctgt tcttttaact caaccggcgt cgaaaatatt
      481 tcctgattag tgcatcatag tcttcttttt tctttctcaa ccatcaaacc aaacacaaca
      541 ttcacaacca cacaataaaa caaaaccaaa acataagatt aacaatgcca gtcaacagga
      601 aaacactgaa tattgaggtg cgctgtaacc tggacagcgc atacggcgca tgtgatcaac
      661 acagcccatt cttttctgtc atcccccaaa tgcagaacaa a
//
LOCUS       DAZFFE010000328          698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFFE010000328 DAZFFE010000000
VERSION     DAZFFE010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.331"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            67..450
                     /locus_tag="ACHJ69_005035"
     CDS             67..450
                     /locus_tag="ACHJ69_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaA family stress response protein"
                     /protein_id="HFO5017801.1"
                     /translation="MSVLYIQIRRNQITVRDLESKREVSGDAAFSNQRLLIANFFVAE
                     KVLQDLVLQLHPRSTWHSFLPAKRMDIVVSALEMNEGGLSQVEERILHEVVAGATLMK
                     YRQFHIHAQSVVLSDSAVLAMFKQK"
     gene            complement(514..>698)
                     /locus_tag="ACHJ69_005036"
     CDS             complement(514..>698)
                     /locus_tag="ACHJ69_005036"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5017802.1"
                     /translation="GRMLVKHALSMAPELTTNVNEQNEQAVGFYKKVGFKVTERSEVD
                     DLGKPYPLLNLAYVGV"
BASE COUNT          188 a          173 c          154 g          183 t
ORIGIN      
        1 aaaaaccccg gtcaataagg ccggggtttt ttgcatatca attatttgca tgatgaaggg
       61 aatctcatgt cagttctgta tatccaaatt cgtcgtaatc aaattactgt tcgcgatctt
      121 gaaagcaaac gtgaagtgtc aggagatgct gccttcagta accagcgcct gttaatcgcc
      181 aatttctttg ttgcagaaaa agttctgcaa gatcttgttc tgcaactcca cccacgttca
      241 acctggcatt cttttttgcc agcaaaacgt atggatattg ttgtgagcgc gctggaaatg
      301 aatgagggcg gtttgtcaca ggttgaggaa cgcattcttc atgaagtggt cgcaggggca
      361 acgttaatga aatatcgcca gttccacatc catgcgcaat cagtggtact cagtgatagt
      421 gcggtcctgg caatgtttaa gcagaaataa tcatgtcacc attggtgggt acgaaaccta
      481 aagttcagcc caccgggatg agaaaaaaac cgcctacacc cccacatatg ccagattcag
      541 caacggatac ggtttcccca aatcgtccac ctcagagcgt tccgtaacct taaagcccac
      601 cttcttatag aacccaaccg cctgctcatt ttgctcatta acattggttg tcagttccgg
      661 ggccattgag agcgcatgct tcaccagcat ccggccca
//
LOCUS       DAZFFE010000329          677 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZFFE010000329 DAZFFE010000000
VERSION     DAZFFE010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.332"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..602)
                     /locus_tag="ACHJ69_005037"
     CDS             complement(<1..602)
                     /locus_tag="ACHJ69_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO5017803.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDDNVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTNKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDE"
BASE COUNT          176 a          166 c          153 g          182 t
ORIGIN      
        1 ttttcgtcgc aggcttcaat catgcgtttc gggtccataa acaactgacc ggggcgcata
       61 gggatctcac gcaactccac atcccagtag cgggcgaatt tatgccagca gatttgtacc
      121 ggaccgcaca ccaggttcgg tttgttagtc ggcttacctg cagcttccat acgcttgcgc
      181 caacgccatt tcatcgccat cccgccgagc atacaggcct cggaagaacc aatggtgttg
      241 gtgccaacgg cctgaccatt tttcggtgca ggcgcatgcc acagatcggc aaccatgttt
      301 acgcaacgca gatcgattgc tgcagattgc ggatattctt ctttgtcgat ccagtttttg
      361 ttaatggata aatccatcaa cttatggaca ttatcgtcgt cccaggtctg gcagaaagtg
      421 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      481 acatcatcac gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      541 ttcgcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      601 attttaaact ccttaaaatg attggatcgc attaaaaaag taggatttat cgataaagta
      661 agcaagtgca aaggact
//
LOCUS       DAZFFE010000330          659 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFFE010000330 DAZFFE010000000
VERSION     DAZFFE010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.333"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>659
                     /locus_tag="ACHJ69_005038"
     CDS             <1..>659
                     /locus_tag="ACHJ69_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017804.1"
                     /translation="GAMAANKTKVVGKYTIEYMGDDSAKIYETGKSQNDGNGNVLLVN
                     TEKGSVSVVNEKEISDAVNAIKSSTQYAELKKKYNNDNVVDKMIVQHAANIYKYAIKT
                     PSLKLENLEKITGEEIEKIDSDVKKAKEVITSKTATAYNTAIENGVSVDSALAAAKDN
                     NGALLNEFNRLGTNVNDLKNATTFALDADGNITLDENQGSGERYSVKQTVADIKADTT
                     V"
BASE COUNT          261 a          101 c          141 g          156 t
ORIGIN      
        1 ggggctatgg ctgctaataa aactaaagta gttggaaagt atacgattga gtatatggga
       61 gatgactccg caaaaattta tgaaactgga aaatcccaaa acgatggaaa tggaaatgta
      121 cttctggtta atacagaaaa aggtagtgtt tccgttgtaa atgaaaagga aataagtgat
      181 gcggtaaatg caattaaatc aagtacacag tatgcagaat taaaaaaaaa atacaataac
      241 gataatgtcg tagataagat gatagtacag catgcagcaa atatatataa atatgcaatt
      301 aaaacgcctt ctttaaaatt agaaaaccta gagaaaatta ctggtgagga aattgagaaa
      361 atagacagcg atgttaaaaa ggcgaaagaa gttatcacat caaaaacagc tactgcatac
      421 aatacagcca tcgaaaatgg cgtaagcgtt gactcagcgc ttgcggctgc gaaagacaat
      481 aacggtgcat tactgaatga gttcaaccgt cttggtacaa atgtaaatga cctgaaaaat
      541 gccactacat ttgcccttga tgccgatggc aatatcacgc tggatgaaaa tcagggaagc
      601 ggtgagcgtt attctgtaaa acaaacagtc gcagatatta aggctgacac tactgtcta
//
LOCUS       DAZFFE010000331          654 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFFE010000331 DAZFFE010000000
VERSION     DAZFFE010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.334"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            144..284
                     /locus_tag="ACHJ69_005039"
     CDS             144..284
                     /locus_tag="ACHJ69_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5017805.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            341..>654
                     /locus_tag="ACHJ69_005040"
     CDS             341..>654
                     /locus_tag="ACHJ69_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017806.1"
                     /translation="MAPVSGERMDDKILRYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSDDGIPYVLVLLGVCTGSKSVERYLPEVKTLTRLAGGRWAEFHT
                     ARR"
BASE COUNT          151 a          146 c          191 g          166 t
ORIGIN      
        1 ctttcggctg atggctggaa ggtgaacctg aaggcccgat gtggaaaggc cccgagtcaa
       61 acattttgtt taacccgagg ccctaaccat cataccttaa gcaagtagaa ggttagcgcc
      121 tctccactca ggaggcaagc gctatgtcgc aaaaatcgct catcaccgtc acaatttgta
      181 tgacggtaat cttcaccatc tggatgttac acggctcgtt gtgtgagttc cggctgaatt
      241 tgtggggagc ggagtttgcg gcgttcttac agtgtaagca gtaggaaaac cgcgacgggg
      301 acgaaagtcc ccgtcaactg gttgctgagg ttcagccgat atggcacccg tttctggtga
      361 gagaatggac gacaaaattc tccggtatat gcagcgtgtt gtgagaaatt cccgcaaccc
      421 tgaatttatg aatgaagtta aagacgcctg ccttaaaaag caggcgtttt gctttgaagc
      481 gccggatggt tttctggtgc tgcgttctgt gctcagtgac gatggtatcc cttatgttct
      541 ggtgttactg ggcgtgtgta cggggagtaa gagtgttgag cgttatctgc cggaggtgaa
      601 gacattaacc cgcctggctg gcggacgctg ggccgaattt catacggcaa gacg
//
LOCUS       DAZFFE010000332          653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFFE010000332 DAZFFE010000000
VERSION     DAZFFE010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.335"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            143..283
                     /locus_tag="ACHJ69_005041"
     CDS             143..283
                     /locus_tag="ACHJ69_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5017807.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            340..>653
                     /locus_tag="ACHJ69_005042"
     CDS             340..>653
                     /locus_tag="ACHJ69_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017808.1"
                     /translation="MAPVSGERMDDKILRYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSADGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHT
                     ARR"
BASE COUNT          145 a          146 c          192 g          170 t
ORIGIN      
        1 ctttcggctg atggctggaa ggtgaacctg aaggcctgat gtggaaaggc cccgagtcaa
       61 ctttaacgtt aacccgaggc cctgacactt ctttccttga caagtagaag gttagcgcct
      121 ctccactcag gaggcaagcg ctatgtcgca aaaatcgctt atcaccgtca caatttgcat
      181 gacggtaatc ttcaccatct ggatgttaca cggttcgctg tgtgagttcc ggctgaattt
      241 gtggggagcg gagtttgcgg cgttcttaca gtgtaagcag taggaaaacc gcgacgggga
      301 cgaaagtccc cgtcaactgg ttgctgaggt tcagccgata tggcacccgt ttcaggtgag
      361 agaatggacg acaaaattct ccggtatatg cagcgtgttg tgagaaattc ccgcaaccct
      421 gaatttatga atgaagttaa agacgcctgc cttaaaaagc aggcgttttg ttttgaggca
      481 cctgatggct ttctggtgct gcgttctgtg ctcagtgctg atggtatccc ttatgttctg
      541 gtgttgctgg gcgtgtgtac ggggagtaac agcgttgagc gttatctgcc ggaggtgaag
      601 acattaaccc gtctggctgg cggacgctgg gctgaatttc atacggcaag gcg
//
LOCUS       DAZFFE010000333          646 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZFFE010000333 DAZFFE010000000
VERSION     DAZFFE010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.336"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            264..443
                     /gene="yciG"
                     /locus_tag="ACHJ69_005043"
     CDS             264..443
                     /gene="yciG"
                     /locus_tag="ACHJ69_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO5017809.1"
                     /translation="MTEYRGGSGNFAEDREKASNAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            529..>646
                     /locus_tag="ACHJ69_005044"
     CDS             529..>646
                     /locus_tag="ACHJ69_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HFO5017810.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEK"
BASE COUNT          228 a          140 c          115 g          163 t
ORIGIN      
        1 acttacgtct tttttccgcc caacagtaat ataatcaaac aaattaatcc cgcaacataa
       61 caccagtaaa atcaataatt ttctctaagt cacttattcc tcaggcaatt gttaatacat
      121 ccagaacgtt cctcaaaata tatttttcca ctttcttctc gttacgctta atttgactaa
      181 ttctcattaa cgactaattt taatgagtgt cgacacacaa cactcatatt aatgaaacaa
      241 tgcaacgcaa cgggagaaat aacatgaccg aatatcgtgg tggttcagga aatttcgccg
      301 aagaccgtga gaaggcatcc aacgcaggcc gtaaaggcgg tcagcatagc ggcggtaatt
      361 ttaaaaatga tccgcaacgc gcatccgaag caggtaaaaa aggcggtcaa caaagcggtg
      421 gtaataaatc aggaaaatcc tgattcaccg ttaattatta atatcagctg ataaagataa
      481 ttgcctctgc gggccgtatg gctcgcagtg atttcactgg agaaaaatat gaatatgaag
      541 accattgaag atgtatttat tcacctgctt tcagatacct acagcgcaga aaaacaatta
      601 acccgggcac tggcaaaact cgcaagagca acatcaaacg aaaaat
//
LOCUS       DAZFFE010000334          642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFFE010000334 DAZFFE010000000
VERSION     DAZFFE010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.337"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          178 a          105 c           78 g          281 t
ORIGIN      
        1 atttatacta ttgaaagttt tactgttatc ttaatgtatt ttttctgtta ttatgtggca
       61 agcaatggta aatacaaaat tattgtatat tgctccatta ttttttcggt tgtgttcact
      121 tactttgtat ttggtgagtt tttcatctca cacaatattt tacctacggt taactttcca
      181 ttattttgtt cttcagcaat atattttctg ctgattttct ttttattctc actttgctca
      241 aagtatggtt tatatggtgt tataattcac tcttcaactg tgtgtgcttt tagttttttg
      301 ggactcatac ctccactaaa cccattaata gcgttatatt attcctattc ttcgtatctg
      361 ccacaaactg aaatccctat agttaattta cttatattaa gtacaatacc aactgttatt
      421 ttttgtaata ctcgcactct taataaacta atatttatat cacttttaat tctcatgata
      481 agtcttagtc agtacaatac gaatgttaat cataaaaaaa atattaaaat agccgttgtt
      541 caggttggtc tttattttga gcagcatgat agcatttctt cattttttca ggactttcac
      601 tcttttttaa aatcaaaccc agacattgat atggttgcat tt
//
LOCUS       DAZFFE010000335          629 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZFFE010000335 DAZFFE010000000
VERSION     DAZFFE010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.338"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>629
                     /locus_tag="ACHJ69_005045"
     CDS             <1..>629
                     /locus_tag="ACHJ69_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO5017811.1"
                     /translation="IIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGS
                     ALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRV
                     ERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERG
                     QVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTG"
BASE COUNT          151 a          149 c          176 g          153 t
ORIGIN      
        1 atcatcgtgt tcctgaacaa atgcgacatg gttgatgacg aagagctgct ggaactggtt
       61 gaaatggaag ttcgtgaact tctgtctcag tacgacttcc cgggcgacga cactccgatc
      121 gttcgtggtt ctgctctgaa agcgctggaa ggcgacgcag agtgggaagc gaaaatcctg
      181 gaactggctg gcttcctgga ttcctacatt ccggaaccag agcgtgcgat tgacaagccg
      241 ttcctgctgc cgatcgaaga cgtattctcc atctccggtc gtggtaccgt tgttaccggt
      301 cgtgtagaac gcggtatcat caaagttggt gaagaagttg aaatcgttgg tatcaaagag
      361 actcagaagt ctacctgtac tggcgttgaa atgttccgca aactgctgga cgaaggccgt
      421 gctggtgaga acgtaggtgt tctgctgcgt ggtatcaaac gtgaagaaat cgaacgtggt
      481 caggtactgg ctaagccggg caccatcaag ccgcacacca agttcgaatc tgaagtgtac
      541 attctgtcca aagatgaagg cggtcgtcat actccgttct tcaaaggcta ccgtccgcag
      601 ttctacttcc gtactactga cgtgactgg
//
LOCUS       DAZFFE010000336          624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFFE010000336 DAZFFE010000000
VERSION     DAZFFE010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.339"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..492
                     /locus_tag="ACHJ69_005046"
     CDS             <1..492
                     /locus_tag="ACHJ69_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5017812.1"
                     /translation="RTVAVEEAKNLLSKGGEIAVRFKIPDGSELVNGQFVFGLYWPVS
                     QWASGAAANSMLASFFLQTDASNLNLMHHKGTSNAQLGTFGAFDHNWHTVVFRFAGNN
                     SERVVPVIDGTEQTAFDLVMWTNDGFTADTLTLTDITGAKATYPVLLDTVTVKVNESR
                     ASS"
BASE COUNT          174 a          130 c          174 g          146 t
ORIGIN      
        1 cgtactgttg cagtggagga ggcaaaaaat cttctcagca aggggggtga aattgccgta
       61 cggttcaaaa ttccggatgg aagcgaactg gtgaacggac agtttgtctt tggtctctac
      121 tggccggtgt cgcagtgggc gtcaggcgcg gcagcaaaca gtatgctggc atcattcttc
      181 cttcagacgg atgcatcaaa tctgaatctt atgcaccaca agggcacgtc gaatgcacaa
      241 ctgggtacat ttggtgcgtt tgaccataac tggcatacgg ttgttttccg ctttgcggga
      301 aataacagcg aaagagtcgt tccggtgatt gatggtacag agcagacggc gtttgacctt
      361 gtgatgtgga caaatgatgg ctttacagca gatacgctga cgctgacaga tatcacgggg
      421 gcaaaagcga cgtatcctgt actgcttgat acggtcacag tcaaagttaa cgaaagccgg
      481 gcatcatcat aaccggcaaa aaaagccgcc agcggcagga acggaagctg gcggaggtaa
      541 tcccaatgga gaatgtaaag aaaagatgct ttcgtatatc ggttttttaa tgaaaacagt
      601 tctcattgtc aaccataacg gtaa
//
LOCUS       DAZFFE010000337          608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFFE010000337 DAZFFE010000000
VERSION     DAZFFE010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.340"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            67..306
                     /locus_tag="ACHJ69_005047"
     CDS             67..306
                     /locus_tag="ACHJ69_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017813.1"
                     /translation="MSQYPELIAQFSSGNQTRIKQGLIAKAPLEGWHYGTKEIVKKFH
                     MYHRVAIEYSGGIYDIDNLRRAHLICMMKFITGDS"
     gene            307..597
                     /locus_tag="ACHJ69_005048"
     CDS             307..597
                     /locus_tag="ACHJ69_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFO5017814.1"
                     /translation="MYNFKDEIEDYTEREFIELLGEFTNPTGDNAQLKGEELDKYWDD
                     LEEHLTRITQHPLMSDLIYYPAKKGDDEPENILKIVKEWRRSQGLPLFKDSK"
BASE COUNT          211 a          100 c          130 g          167 t
ORIGIN      
        1 aaatactgaa gcctcattag gttattttat cattttcccg cttttaccga taccctttgg
       61 ctggaggtga gtcagtatcc ggagttgata gcgcagtttt caagcggtaa tcaaacacgt
      121 ataaaacaag gattaatcgc aaaagcacca cttgaaggtt ggcattatgg cactaaagaa
      181 atcgttaaaa aattccacat gtatcatcgt gttgcgattg aatatagtgg cggaatttac
      241 gatattgata atttaaggag ggcacacctc atttgcatga tgaaatttat tacaggagat
      301 agttaaatgt ataattttaa agatgaaata gaagattaca cagagcggga attcattgag
      361 ttgcttggcg agtttacaaa tccaaccggg gataacgctc aattaaaagg ggaagagctt
      421 gacaaatatt gggatgatct agaagaacat cttaccagaa ttacacaaca tccgttgatg
      481 agcgatctta tctactatcc tgcaaagaag ggagatgatg aacctgaaaa catcctgaaa
      541 attgtcaaag aatggcggcg ttcacagggg ctacccttgt ttaaagactc taaataaagt
      601 agataata
//
LOCUS       DAZFFE010000338          603 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZFFE010000338 DAZFFE010000000
VERSION     DAZFFE010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.341"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>603
                     /locus_tag="ACHJ69_005049"
     CDS             <1..>603
                     /locus_tag="ACHJ69_005049"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO5017815.1"
                     /translation="NDCLSSINSQILKPNQIVIVYDGPIRKELDDIVTRWEKQLPIHC
                     VKLTSCTGLGNALNYGLKFCDYDIVCRMDTDDICRNDRFLRQVKILESDNNLALIGSY
                     ISEFDSTPSNSHAVRTVPIEHNDICLYAKKRNPFNHMTVAFRKSVVTSLGGYKDEYLY
                     EDYALWIKMLKNGFLTKNIPEALVYARVGNNMELKRGGWK"
BASE COUNT          205 a           80 c          126 g          192 t
ORIGIN      
        1 taatgattgc ttaagtagca ttaattcgca aatattaaaa ccgaaccaaa ttgtaatcgt
       61 atatgatggc ccaattagaa aagaattgga cgatatagtt acaagatggg aaaagcaatt
      121 gccaatacat tgtgtaaagc tcacttcctg tacaggatta ggtaatgcat tgaattatgg
      181 tttgaaattt tgtgattacg atattgtctg tcgtatggac actgatgata tatgtagaaa
      241 tgacagattt ttaagacaag ttaaaatact tgaaagtgat aataatctgg ctttgattgg
      301 gtcttatatc tcagagtttg actctactcc ctcaaacagt catgctgtta ggaccgttcc
      361 cattgagcat aatgatattt gtttatatgc aaaaaaaaga aatccgttta accatatgac
      421 tgtagcattt agaaaaagtg tagtaacttc tctcggtggg tataaggatg aatatttata
      481 tgaagactac gcactgtgga ttaagatgct taaaaatgga tttttgacta aaaatattcc
      541 agaggcgcta gtttatgcgc gggttggtaa taatatggag cttaaacgcg gtgggtggaa
      601 ata
//
LOCUS       DAZFFE010000339          584 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFFE010000339 DAZFFE010000000
VERSION     DAZFFE010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.342"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..237
                     /locus_tag="ACHJ69_005050"
     CDS             <1..237
                     /locus_tag="ACHJ69_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO5017816.1"
                     /translation="VAAAKAGKLPEEFFWTDADNNDVPMDAETLIALSAAAEQAMFTK
                     GLEIHVRQRTMKKEIEALSGAEAILAYKVGITDG"
     gene            complement(360..>584)
                     /locus_tag="ACHJ69_005051"
     CDS             complement(360..>584)
                     /locus_tag="ACHJ69_005051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017817.1"
                     /translation="IEGRTKLFSAIPQPTKILYLMIVLFALFLCSHSVAYKKAPLSAP
                     SSQYLCRWIKITINHKYHALGEHALPVFYL"
BASE COUNT          190 a          104 c          156 g          134 t
ORIGIN      
        1 gtggcggcag cgaaagcggg gaaactgccg gaggagtttt tctggacgga tgcggacaat
       61 aatgacgtac cgatggacgc tgaaacactg attgctctga gtgctgctgc agagcaggcg
      121 atgtttacca aaggtctgga aattcatgtc agacagcgca cgatgaaaaa agagattgaa
      181 gcgctgagtg gtgcggaagc tatactggcg tataaagtcg gtatcacaga cggctgataa
      241 aaaagagagg ggcaagccat agccccttgc tacaaatcta cagctaaata ataccctggt
      301 tgaggcaaat tattcggtaa taaaaattta aggaggcaaa atagcctcct tcacccctgt
      361 caaagataaa aaacggggag tgcgtgctca ccgagagcat gatacttgtg gttgatagtt
      421 atttttatcc agcgacaaag atattgagat gagggcgcgg atagtggtgc ttttttataa
      481 gcaacagaat ggctgcataa aaagagagca aataaaacaa tcatcaaata cagaattttc
      541 gtaggctgag ggattgctga aaataattta gtgcgtcctt caat
//
LOCUS       DAZFFE010000340          567 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZFFE010000340 DAZFFE010000000
VERSION     DAZFFE010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.343"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>567
                     /locus_tag="ACHJ69_005052"
     CDS             <1..>567
                     /locus_tag="ACHJ69_005052"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
                     /protein_id="HFO5017818.1"
                     /translation="VIEGDLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHI
                     DVGLFRAASEGVPQGGVISPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGR
                     STAVRENWQWKPAVAYCRYADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKT
                     KITHVNDGFIFLGHRIIRKRSRYGEMRVV"
BASE COUNT          145 a          132 c          168 g          122 t
ORIGIN      
        1 gtgattgaag gcgacctgtc cagttacttc gacaccgtac atcatcgact gctgatgaag
       61 gctgtacgcc gcaggatcag tgacgcacgt ttcatgactc tgctgtggaa aaccatcaag
      121 gcgggacata tcgatgtcgg tctctttcgg gcggccagtg aaggtgtgcc acagggcggt
      181 gttatatcgc cgctattatc gaacatcatg ctgaatgagt tcgatcaata cctgcatgag
      241 cgctacctga gcgggaaagc cagaaaagat cggtggtact ggaataacag tatccaacgg
      301 ggccgaagta cggcggtcag agaaaactgg cagtggaaac ccgcggtggc gtactgccgc
      361 tatgccgatg attttgtcct catcgtcaaa ggcaccaaag cacaggcgga agccatcagg
      421 gaggagtgtc ggggtgtgct cgaaggcagt ctgaaactca ggctgaacat ggataagact
      481 aaaatcaccc atgttaatga cggctttatc tttctggggc acaggatcat tcgcaaacgc
      541 agtcgttatg gcgagatgcg agtggtc
//
LOCUS       DAZFFE010000341          557 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFFE010000341 DAZFFE010000000
VERSION     DAZFFE010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.344"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>557)
                     /locus_tag="ACHJ69_005053"
     CDS             complement(<1..>557)
                     /locus_tag="ACHJ69_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO5017819.1"
                     /translation="DADIACKGSIKELLDYQFSTNEIAAVVAERAVEWWQNRASVLTT
                     PQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNGKV
                     KFISGKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKA
                     ASPWSKEDLLKPVNSNQYRYCAKHK"
BASE COUNT          172 a          116 c           87 g          182 t
ORIGIN      
        1 aatttatgtt ttgcacaata ccgatactga ttgctattta caggtttaag taaatcttct
       61 ttactccacg gagaagctgc tttggcgatc aaaaaactac gtgacaccgg ataatcagcc
      121 cactcatgcc agggttttgt aggtcccaca tagtgtataa acacggtgtc atcattgact
      181 ggattatcaa ctttgtcttt taattcatag ttaatactat atcgggtatt atatttcccc
      241 gaaataaact tcactttacc attcaataat acattcagta catcttgatc aaggtgggtg
      301 attttactta cccaatctgg gtcacgcaac atttcaatag cttttgacga aatgttattt
      361 agattccact catcaatatt tatcagtaaa aaaccagcat tgaaatatcc agaagctaat
      421 tgtggtgtag ttaataccga ggctcgattc tgccaccatt caacagctct ttcagccaca
      481 actgcggcaa tttcattagt agaaaattga taatctaaga gttctttaat actacccttg
      541 caagcaatat ctgcatc
//
LOCUS       DAZFFE010000342          557 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZFFE010000342 DAZFFE010000000
VERSION     DAZFFE010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.345"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>557
                     /locus_tag="ACHJ69_005054"
     CDS             <1..>557
                     /locus_tag="ACHJ69_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5017820.1"
                     /translation="HIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWDML
                     THPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLAQQRKAWDV
                     LSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSALKDRH
                     TAVEVNYTDPQNGWQTSTELVEDPEA"
BASE COUNT          118 a          149 c          188 g          102 t
ORIGIN      
        1 catatccgcg gtcgcatcat ccaggtgccg tcaaactatg acccggaaaa acgcacgtac
       61 agtggtatct gggacggcag tctgaaaccg gcatacagta acaatccggc ctggtgtctg
      121 tgggacatgc tgactcaccc gcgctacggc atgggaaaac gtctgggggc ggcggatgtg
      181 gacaagtggg cgctgtatgc catcgggcag tactgcgacc agacggtccc ggatggtttc
      241 gggggcacag agccgcggat gacctttaat gcgtacctgg cacaacagcg taaggcgtgg
      301 gacgttctca gtgatttctg ctcggcgatg cgctgtatgc cggtatggaa cggccagacg
      361 ctgacgttcg ttcaggaccg cccgtcggat gtggtgtggc cgtacaccaa cagcgatgtg
      421 gtggtggatg ataacggcgt ggggttccgc tacagcttca gtgccctgaa ggaccggcac
      481 acggcggtgg aggtgaatta caccgacccg cagaacggct ggcagacctc cacggaactg
      541 gtggaagacc cggaagc
//
LOCUS       DAZFFE010000343          545 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZFFE010000343 DAZFFE010000000
VERSION     DAZFFE010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.346"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>545
                     /locus_tag="ACHJ69_005055"
     CDS             <1..>545
                     /locus_tag="ACHJ69_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017821.1"
                     /translation="KDVDQRNFDYRAKVDDRNYALQEREFNANQNYRNASLGMEQQRL
                     RMQKYNQRRLEYNDMLARDQPVMAALGKAVDAGDRDAAMRLYGQLSEGNPLRLMANDG
                     YVAKAGQAVNNLQKIFDDKPDRAIASLNTPENLDVLSGVFGPELQQRIGMPDSTGKKT
                     IKEARIGSIVPAQQEGYILIGL"
BASE COUNT          149 a          121 c          165 g          110 t
ORIGIN      
        1 aaagacgttg atcagcggaa ctttgattac agagccaaag tcgacgaccg taattatgca
       61 ctgcaggaaa gggagtttaa cgccaaccag aattaccgga atgcgtcgct gggtatggag
      121 cagcagcgac tccggatgca gaaatacaac cagcgacggc ttgagtataa cgatatgctg
      181 gcgcgcgatc agcctgtgat ggcggcgctt ggaaaggctg tggatgctgg cgatcgggat
      241 gcagcaatgc gtctttacgg ccagttgtca gagggtaatc cgctgaggct gatggcgaat
      301 gatggctatg tagcgaaagc gggtcaggcc gtgaacaacc tgcaaaaaat ctttgatgac
      361 aagccagaca gggctatcgc ttcacttaat acgccggaga atcttgatgt cctttccggg
      421 gtgtttggcc cggaactgca acagcgtatt ggcatgcccg attcaaccgg gaaaaagacg
      481 ataaaagagg ccaggattgg cagtatagta ccggcacagc aggaagggta catacttatc
      541 ggcct
//
LOCUS       DAZFFE010000344          537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFFE010000344 DAZFFE010000000
VERSION     DAZFFE010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.347"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..256)
                     /locus_tag="ACHJ69_005056"
     CDS             complement(<1..256)
                     /locus_tag="ACHJ69_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5017822.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMSGKGSSARPSLTVSNLF"
     gene            complement(256..>537)
                     /locus_tag="ACHJ69_005057"
     CDS             complement(256..>537)
                     /locus_tag="ACHJ69_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5017823.1"
                     /translation="QPSVREVRFGDGYSQRMAAGLNADLKTYRVMLSVTREEARHLEA
                     FLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSAEFKQVVN"
BASE COUNT          116 a          192 c          112 g          117 t
ORIGIN      
        1 caaacagatt ggacaccgtc agcgacggtc tggcactgct gcccttcccg ctcatctcaa
       61 aaccgctgcc gtcaatcggg tatgcctcat acttacgccc ctgccaggtg accggctccc
      121 ctttttcatt cagctcatta cagaaaaaat accgctcacc gccctgtacc gtcaggtcga
      181 tttcccagag taccacccgc ggtgactgct ctgatttaac cgactcgttc agactttctt
      241 cgtgaatatc ctgcatcagt tcaccacctg cttaaactcc gcgctgaact caacgcgcaa
      301 catcccgacc cgcgcagacc acccggcaca ggtcaccttt atctgccggt atgcataggg
      361 tggcttccac aaaaatgcct tccagccacc gtgctctgcc agaaacgctt ccagatgccg
      421 ggcctcctcc cgggtcacgg aaagcatcac ccggtatgtt ttcaggtcag cattcagccc
      481 tgccgccata cgctgtgagt acccgtcacc aaaacgcact tcacgcaccg atggctg
//
LOCUS       DAZFFE010000345          533 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZFFE010000345 DAZFFE010000000
VERSION     DAZFFE010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.348"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>533)
                     /locus_tag="ACHJ69_005058"
     CDS             complement(<1..>533)
                     /locus_tag="ACHJ69_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO5017824.1"
                     /translation="VIVTTVAQGQPGETGSYSFDVEPGWYRVTLNTEGYAPSYVGDIL
                     VKADSEPGTLNKFLMEQDEAQYYPKALAELEAVAAEILKRAEASAASAEEAKKRAENA
                     RGPAGEKGDTGPQGATGAQGPAGATGAVGPKGEPGPKGERGETGPQGPKGDKGDPGGP
                     PGPKGDTGPRGEAGPPG"
BASE COUNT           60 a          217 c          125 g          131 t
ORIGIN      
        1 gtccgggtgg tccggcctct ccacgtgggc cggtgtcacc tttcggcccc ggcggtccgc
       61 ccgggtcacc tttatcgccc ttcggtccct gtggacctgt ttctccccgt tctccctttg
      121 gccccggctc accttttggt ccgaccgccc ccgttgcccc ggctggtccc tgtgcccctg
      181 tggcaccctg tggacctgtg tcccccttct cgcccgccgg tccccgggca ttctctgccc
      241 gtttcttcgc ttcctctgca ctcgccgccg acgcttcagc acgcttcagg atttccgctg
      301 ccaccgcttc cagctctgca agcgctttcg ggtaatactg cgcctcatcc tgttccatca
      361 gaaatttatt cagcgttccc ggctcagaat ccgccttcac cagaatgtca cccacatacg
      421 acggggcgta cccttccgtg ttcagcgtca cccggtacca ccccggctcc acatcaaaac
      481 tgtaactgcc ggtttccccc ggctgcccct gcgccaccgt ggtgactatc act
//
LOCUS       DAZFFE010000346          521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFFE010000346 DAZFFE010000000
VERSION     DAZFFE010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.349"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..521
                     /locus_tag="ACHJ69_005059"
                     /pseudo
     CDS             1..521
                     /locus_tag="ACHJ69_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
BASE COUNT          122 a          128 c          158 g          113 t
ORIGIN      
        1 gatatggcca tgaatggcgt tggatgccgg gcaactgccc gcattatggg cgttggcctc
       61 aacacgattt tacgtcactt aaaaaactca ggccgcagtc ggtaacctcg cgcatacagc
      121 cgggcagtga cgtcatcgtc tgcgcggaaa tggacgaaca gtggggctat gtcggggcta
      181 aatcgcgcca gcgctggctg ttttacgcgt atgacagtct ccggaagacg gttgttgcgc
      241 acgtattcgg tgaacgcact atggcgacgc tggggcgtct tatgagcctg ctgtcaccct
      301 ttgacgtggt gatatggatg acggatggct ggccgctgta tgaatcccgc ctgaagggaa
      361 agctgcacgt aatcagcaag cgatatacgc agcgaattga gcggcataac ctgaatctga
      421 ggcagcacct ggcacggctg ggacggaagt cgctgtcgtt ctcaaaatcg gtggagctgc
      481 atgacaaagt catcgggcat tatctgaaca taaaacacta t
//
LOCUS       DAZFFE010000347          520 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZFFE010000347 DAZFFE010000000
VERSION     DAZFFE010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.350"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>520
                     /locus_tag="ACHJ69_005060"
     CDS             <1..>520
                     /locus_tag="ACHJ69_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO5017825.1"
                     /translation="GGYKGKAAQNIHFVPLMTDEHGVNVPTNEPSEDPDIIPAGYYGA
                     ASRTASNWTSADRKTHFSSWARRGLVSDRLAGAILQYAGRTLSFLTGQSAPQSGGTTP
                     VSPGTPDVEKPQDGGVAGTGHDEAVSSTRTVAEYDANSGNGVWTEQQWGAAGGKGTVT
                     DDGGRKALRLEKQ"
BASE COUNT          122 a          118 c          177 g          103 t
ORIGIN      
        1 cggtgggtat aaaggcaaag ctgcgcagaa tattcacttt gtgccgttga tgacggatga
       61 gcatggtgtg aatgtgccga caaacgagcc gtcagaagat ccggacatta tcccggcggg
      121 atactatggt gctgcgtcac gcactgccag taactggacg tcagccgatc gtaaaacgca
      181 cttcagttca tgggcgcgaa gaggccttgt ttcagaccgc ctggcaggtg ccattttgca
      241 gtacgccggg cggacattat cgttccttac cgggcagagc gcaccgcagt cgggtggtac
      301 aactcctgtc agtccgggga caccggatgt tgagaaaccg caggatggcg gtgttgctgg
      361 tactggtcat gatgaggccg tgagcagtac caggacggtg gctgaatatg atgcgaacag
      421 tggaaatggt gtatggacag agcagcagtg gggggctgcc ggtggcaaag gcactgtgac
      481 tgatgacggt ggacggaaag cgctgcgact ggaaaaacag
//
LOCUS       DAZFFE010000348          519 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZFFE010000348 DAZFFE010000000
VERSION     DAZFFE010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.351"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>519
                     /locus_tag="ACHJ69_005061"
     CDS             <1..>519
                     /locus_tag="ACHJ69_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017826.1"
                     /translation="EKVVLGAINTTVGSVMTWNKQDAEKVQKIIEKLPEKQQQVGTQI
                     AMQRIVFPVDSPSLKLDKLKSGTLTKADIDAVVSLKTDVSNAISIETAPAYVSAVRSG
                     GNSDAALAAAKHGPGALQEYNRIVTNIDRLNKDTTFAIDANGNITLDETGSVERESVK
                     NVVAAIQADTTI"
BASE COUNT          172 a           96 c          115 g          136 t
ORIGIN      
        1 tgaaaaagtt gtattaggtg ctataaatac aactgtaggt agtgtcatga cttggaacaa
       61 acaggatgct gaaaaagttc aaaaaatcat tgaaaaattg ccagaaaaac aacaacaagt
      121 tggtacacaa attgcgatgc aacgaattgt gttcccggtt gattctccta gtttaaagct
      181 tgataagttg aagtcaggaa cattaaccaa ggcggatatt gatgctgttg tatccttaaa
      241 aacagatgtt tcaaatgcta tttctatcga aacagcgcca gcgtatgtgt ctgcggttcg
      301 ttcaggggga aactctgatg ccgcgcttgc tgctgctaaa cacggcccag gtgctctgca
      361 ggaatacaac cgcatcgtta ccaatataga taggttgaat aaagacacaa cttttgctat
      421 cgatgctaac ggtaacatta cccttgatga aacaggctca gtggagcgag agtctgtaaa
      481 aaatgtagtt gcagccattc aggcagatac aacaattta
//
LOCUS       DAZFFE010000349          502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZFFE010000349 DAZFFE010000000
VERSION     DAZFFE010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.352"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..431
                     /locus_tag="ACHJ69_005062"
     CDS             <1..431
                     /locus_tag="ACHJ69_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HFO5017827.1"
                     /translation="TALLSGNYSESKVDMAEQLNRKLKGWAMFYQFVDFKAKVFSYID
                     RVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNHGKLSGEILYRLV
                     GQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          136 a          113 c          145 g          108 t
ORIGIN      
        1 tgacagcact gttatcaggc aactacagtg aaagcaaagt cgatatggct gaacaactca
       61 accgaaaact gaaaggctgg gccatgttct atcagttcgt tgattttaag gccaaagtct
      121 tcagttatat cgaccgtgtc gtgttctgga agctggctca ctggctggcc cgcaaatacc
      181 gtacaggtat cgcttccctg atgaggtggt ggtgtaaatc accgaaaccg ggtcagagca
      241 aaacgtgggt tttatttggt aaaaccaatc acggcaagct cagcggcgaa atactgtacc
      301 ggttggtggg gcaaggcaag aagctgttcc gctggcggct acccgaaggt aatccctatc
      361 tgaggacgga gaccagaaac acgtatacat cgcgctttac agaagtggca atggcgttcg
      421 ccagcattta aatggagagc cggatgcgct gaaaggtgca cgtccggttc ggggaggaga
      481 ggcagggaaa tagtccgact ac
//
LOCUS       DAZFFE010000350          497 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZFFE010000350 DAZFFE010000000
VERSION     DAZFFE010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.353"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            220..>497
                     /locus_tag="ACHJ69_005063"
     CDS             220..>497
                     /locus_tag="ACHJ69_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HFO5017828.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPED"
BASE COUNT          156 a           85 c           90 g          166 t
ORIGIN      
        1 ttaatataac gagttgcatt tagttacatg tttaacactt gatttaagat ttgtaatggc
       61 tagattgaaa tcagatgtaa tccattagtt ttatatttta cccatttagg gctgatttat
      121 tactacacac agcagtgcaa catctgtcag tacttctggt gcttctattt tagaggcagc
      181 tgtcaggtgt gcgatcaata aaaaaagcgg ggtttcatca tgtttaatga agtccatagt
      241 attcatggtc atacattatt gttgatcact aaaccttctt tgcaggcgac agctctcttg
      301 cagcacctta aacaatcgct ggcaattaca ggaaaattac ataatattca acgttctctg
      361 gacgatatat cttcaggctc tattattctt ctggatatga tggaagcgga taaaaagctt
      421 atccattatt ggcaggatac tttgagcagg aaaaacaaca atatcaaaat attgttgcta
      481 aatacgcctg aagatta
//
LOCUS       DAZFFE010000351          491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFFE010000351 DAZFFE010000000
VERSION     DAZFFE010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.354"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          183 a          104 c           77 g          127 t
ORIGIN      
        1 tgttttcatt ttattcgtct gacgtgttcc aattatttag caaaactgcg acaacaaagt
       61 gatggtgtaa tttgtaaaag ggaacaaata aatggctcgc cctgcaggat tcgaacctgt
      121 agcccacggc ttagaaactc caggaacgac attttaagtc aacaaattac cgcgccatct
      181 ctgcgttcac acgtcccact acctcaaaat atgtaaaacc ttgcaagcca ttgcgaggcc
      241 ttatgtgtct cagttttgtt cctctttttt atactaaaaa acacagcaat tgaggataaa
      301 cctcatgcta ttatcgctaa tatgcatcta aaggcatggc acttgaatag ataaaaactc
      361 cacaaagaaa aaataattag cccataaaac cacaatgcaa aacacaaaca gacaattaaa
      421 ctaaaaaact gacagtgcat tgtgacaaaa attcaatgtt aaacaaaata taaacagtgg
      481 ggcaatatac a
//
LOCUS       DAZFFE010000352          490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZFFE010000352 DAZFFE010000000
VERSION     DAZFFE010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.355"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(85..>490)
                     /locus_tag="ACHJ69_005064"
     CDS             complement(85..>490)
                     /locus_tag="ACHJ69_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5017829.1"
                     /translation="QKEEVIAITNWLNERNSLNVKHFNDNPWLFVSRTGKPLSRQRFY
                     NIVSAAGKNAGLNIKVHPHMLRHACGYSLADNGVDTRLIQDYLGHRNIRHTVIYTASN
                     SMRFEKMWGRGDAKKQHFDPKCKPNLCLEIFV"
BASE COUNT          150 a           94 c           75 g          171 t
ORIGIN      
        1 aacctacctc tttatgtgta gattgacgcc acacattgat ctaaatcagt aaatttaatg
       61 ttttttatag aaaaaaatgt ttttctatac gaaaatttct aaacaaagat tgggtttaca
      121 ttttgggtca aaatgttgct tttttgcgtc acctctcccc cacatttttt caaatctcat
      181 tgaattagaa gcagtataaa ttaccgtatg cctgatatta cgatgcccga gatagtcctg
      241 aataagacgg gtatccacac cattatctgc tagtgaataa ccacatgcat ggcgtaacat
      301 atgtggatga actttaatat ttaaacctgc atttttaccc gcagcagaaa ctatgttata
      361 aaaccgttgt ctcgataatg gttttcctgt tcgggaaaca aataaccacg ggttatcatt
      421 gaaatgttta acatttaatg aattcctttc attcagccag tttgttattg caatcacctc
      481 ttccttttga
//
LOCUS       DAZFFE010000353          484 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFFE010000353 DAZFFE010000000
VERSION     DAZFFE010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.356"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(5..>484)
                     /locus_tag="ACHJ69_005065"
     CDS             complement(5..>484)
                     /locus_tag="ACHJ69_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO5017830.1"
                     /translation="GQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAA
                     YLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEYPGYTLYDLSERLRLRGWQVPAF
                     TLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
BASE COUNT          114 a          126 c          135 g          109 t
ORIGIN      
        1 gttatcaggt gtgtttaaag ctgttctgct gggcaatacc ctgcagtttc gggtggtcgc
       61 tgagatattt cagggaggct ttgtagtctt ccagcaacag ttcagcaaag tccatttcga
      121 agccgcgacg gcacataatg cgcatcacca cgatgtcggt ggcttcaccg ccgagagtga
      181 aagccggaac ctgccagccg cgcagacgca gacgttcaga gaggtcgtac agggtgtatc
      241 ctggatattc accatctttc agtttgaagc aaaccgccgg gatgccttcg tccgggcgac
      301 cggtacagat gaactcatac ggccccagtt tggcgatttc atccgccaga taagcagcaa
      361 cctggtagga agcgttctgt actttggtat agccttcacg accgaggcgc aggaattcat
      421 aatactgtgc aatcacctga cccgccgggc gggagaagtt gatggcgaaa gtaccaatct
      481 gacc
//
LOCUS       DAZFFE010000354          468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFFE010000354 DAZFFE010000000
VERSION     DAZFFE010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.357"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>468
                     /locus_tag="ACHJ69_005066"
     CDS             <1..>468
                     /locus_tag="ACHJ69_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5017831.1"
                     /translation="ERYIPCVNISAEPEAYFRIAPEDWLRAEMQGEIVALVHSHPGGL
                     PWLSEADRRLQIKSALPWWLVSRGDIHKFRCVPHLTGRRFAHGVTDCYTLFRDAYHLA
                     GIDMPDFHRGDDWWCNGQNLYLDNMEATGFYRVPLSSALPGDILLCCFGASVA"
BASE COUNT           84 a          125 c          156 g          103 t
ORIGIN      
        1 ggagcggtat atcccttgtg tgaatatttc cgcagagccg gaggcgtatt ttcgtatcgc
       61 accggaagac tggctgcggg cagagatgca gggggagatt gtggcactgg tccacagtca
      121 tcccggtgga ctgccctggc tgagcgaggc cgaccggcgg ctgcagataa aaagcgcact
      181 gccctggtgg ctggtcagcc ggggtgacat tcacaaattc cgctgcgttc cgcacctgac
      241 gggacggcgc tttgcgcacg gggtgacgga ctgttacacg ctgttccggg atgcatacca
      301 tctggcaggc attgatatgc cggattttca tcgcggggat gactggtggt gcaacggtca
      361 gaacctttac ctggacaata tggaggcaac gggcttttac cgggtgcccc tgtcctctgc
      421 actgccgggc gatatcctgc tgtgctgctt cggcgcatcg gtggctaa
//
LOCUS       DAZFFE010000355          460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFFE010000355 DAZFFE010000000
VERSION     DAZFFE010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.358"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..192)
                     /locus_tag="ACHJ69_005067"
     CDS             complement(<1..192)
                     /locus_tag="ACHJ69_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFO5017832.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESE"
BASE COUNT          150 a           99 c           70 g          141 t
ORIGIN      
        1 ttcactttca ccggctaaat cagcaatgac taatccggaa cgacgtaatt gtaataaggt
       61 aaggtcctcc gcagaggcgc tggatattat ttcatattca gggaagtgag tcctcatgac
      121 ctcttttaac cctgcttgca taacaggtat tttactgata acaattccat ttttgcagca
      181 tcctggcaac atatttcctc cacatccact gtctaaaaat catgatggtt tattcaaggt
      241 attcactcct taaatattcc cagcaaatta atagattaag cgaataaatt atccttagca
      301 ttcactttca tatcagcata ctcctgctgc acaacaactc cactgcatgt gtcagaaggt
      361 tagcatcaaa tgataacctg gtgaaatagc ctggagacat ttaaaatatc tacattaagt
      421 tgttagaaat acttaatgaa aaacagaaat ctcattcatt
//
LOCUS       DAZFFE010000356          455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFFE010000356 DAZFFE010000000
VERSION     DAZFFE010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.359"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            199..>455
                     /gene="pagP"
                     /locus_tag="ACHJ69_005068"
     CDS             199..>455
                     /gene="pagP"
                     /locus_tag="ACHJ69_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="catalyzes the transfer of palmitate to lipid A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO5017833.1"
                     /translation="MKVSKYVAIFFFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRW"
BASE COUNT          129 a           71 c           98 g          157 t
ORIGIN      
        1 tttttctttt atgactattc ccatcgcaga aaacgatgca tcgtctttaa tcgatgcgcg
       61 gaaatattta tcttgaacta gcggaaataa atagagcagc tattcatatt attcttcatg
      121 ttggggctat tgaggttatg ttaattgtag ctttgctatg ttagtagtag atttttgata
      181 aatgtttaat ggtcacaaat gaaagtgagt aaatatgtcg ctatattttt ctttgttttt
      241 attcagttaa tcagtgttgg taaagttttt gctaacgctg atgagtggat gacaacgttt
      301 agagaaaata ttgcacaaac ctggcaacag ccagaacatt atgatttata tattcctgcc
      361 atcacctggc atgcacgttt tgcttacgac aaagaaaaaa ccgatcgcta taacgagcga
      421 ccgtggggtg gcggttttgg tcagtcgcgt tggga
//
LOCUS       DAZFFE010000357          454 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFFE010000357 DAZFFE010000000
VERSION     DAZFFE010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.360"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>454
                     /gene="wzy"
                     /locus_tag="ACHJ69_005069"
     CDS             <1..>454
                     /gene="wzy"
                     /locus_tag="ACHJ69_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_033808138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O107/O117 family O-antigen polymerase"
                     /protein_id="HFO5017834.1"
                     /translation="IFSMQYSVSARDNIEMGSNIITTFLKQQGVSVNVIGIAIKDKNN
                     SLLSESVILSPLYDSAISLANSLVGVQSNGNSVEFAENSFSLSHKLSYLEDPSAYLAG
                     YGVGGAAIAELYIVGGYLACLIGGMLTYIFISILEKIAKKSFFNFIFV"
BASE COUNT          135 a           66 c           88 g          165 t
ORIGIN      
        1 taatatttag tatgcaatac tcggtttcag caagagataa tattgaaatg gggtcgaata
       61 tcatcacaac gttcttaaaa caacaaggcg tatcagtcaa tgttattggt attgctataa
      121 aggacaaaaa taattcatta ttaagtgaat ctgtgatttt gtcgccacta tatgatagtg
      181 ctatttcttt agctaattct ttagtaggtg tccaaagcaa tggaaattcg gtagagttcg
      241 cagagaattc attctcattg tcgcacaaat tgagttattt agaagatcct tcagcatacc
      301 ttgctggcta tggtgttggg ggagccgcta ttgctgagct gtacatagtt ggtggttact
      361 tggcttgttt aattggaggt atgcttacat atatttttat ctctatacta gaaaaaatcg
      421 ccaaaaaatc cttttttaat tttatttttg ttat
//
LOCUS       DAZFFE010000358          447 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFFE010000358 DAZFFE010000000
VERSION     DAZFFE010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.361"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(70..>447)
                     /locus_tag="ACHJ69_005070"
     CDS             complement(70..>447)
                     /locus_tag="ACHJ69_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017835.1"
                     /translation="TPVPALQPAVLKTLGDEKSGDGSDSGEENEDSGGNCVTGSGGDD
                     RGDDEDEPEKYTLQFHFTDDDGIPYSETRYIAFFEDGTQTRGETDEEGYTERFFVSSK
                     HEIKVKLLFANDDFLSMEGHYGR"
BASE COUNT           95 a          128 c           81 g          143 t
ORIGIN      
        1 tgatctgtta ttttttgctc tttcacacat tcttctggct ctggctttgg ttgtccagaa
       61 gtttgattat tatcgtccat agtgtccttc cattgagaga aaatcatcat tcgcaaatag
      121 caacttcact ttaatttcat gtttgctaga tacaaaaaaa cgctctgtat acccctcttc
      181 atccgtttct ccgcgggtct gcgtgccatc ctcaaagaag gcaatgtagc gggtttctga
      241 atacggtatc ccgtcatcgt ctgtaaaatg gaactggaga gtatattttt caggttcatc
      301 ctcatcgtca cctcgatcat caccaccact tccggtcacg cagttccctc cagagtcttc
      361 attttcttct ccggaatcac tgccatcacc actcttctca tcccccagtg tcttcagcac
      421 cgccggttgc agcgccggca ccggcgt
//
LOCUS       DAZFFE010000359          433 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFFE010000359 DAZFFE010000000
VERSION     DAZFFE010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.362"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>433)
                     /locus_tag="ACHJ69_005071"
     CDS             complement(<1..>433)
                     /locus_tag="ACHJ69_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO5017836.1"
                     /translation="EKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRG
                     STPKIKGSCQIEKAANESAHFMRFYVPCPHCGEAQYLKFGDESTPFGLKWEKDSPESV
                     FYLCEHHGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSAA"
BASE COUNT           95 a          130 c          109 g           99 t
ORIGIN      
        1 ccgcggcgct gaaaaacgtc agaccgtcgc gggtccacat ccccgtgttt tcacagatcc
       61 accgcccgtt gctctggtca agctcagact gatggatcac gcagccatga tgttcacaga
      121 ggtagaaaac gctttcgggg ctgtccttct cccatttaag gccaaaaggc gtggactcat
      181 cgccaaattt cagatactgc gcctccccac agtgcgggca gggcacataa aaacgcatga
      241 aatgcgccga ctcgttggcc gctttttcga tctggcagga gccttttatt ttaggcgtcg
      301 agccgcgaat ggattttggc cataccgagc cctcaatacg cttatccccc agcagggttg
      361 gcgagccctc tttttcgaca tccggctcga acgaggaaag ttcgtcatag cagaccacgt
      421 ccacggattt ttc
//
LOCUS       DAZFFE010000360          428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFFE010000360 DAZFFE010000000
VERSION     DAZFFE010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.363"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          161 a           73 c           69 g          125 t
ORIGIN      
        1 ataagtccta cgacattcga acgagatttc gaaagccatt tttaaaaatt atatcaagta
       61 cgaacaaact tagtgacaaa agactacgaa aaatataaaa caattttagt attaagtaga
      121 gagaaatttc gttctgctag gacgcagcaa taggttcatg agattctctg atacttgtta
      181 ccttagtaag ccttaaattt ttaaaaattc ttttacgtcc actcctcgct cataacaggc
      241 attaactaca tttatcttca ttggctatgc aagtcgtaat aaaaggtggg acaaaactga
      301 gacacataag gtctcacaaa ggtctgcaag gcctcacatg tattgagatg ttaataaaat
      361 gtagacttgt aattttgata taaatggtag ataaaacctt tccccaaaat aaaaacgaac
      421 gtcaatga
//
LOCUS       DAZFFE010000361          420 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFFE010000361 DAZFFE010000000
VERSION     DAZFFE010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.364"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>420
                     /locus_tag="ACHJ69_005072"
     CDS             <1..>420
                     /locus_tag="ACHJ69_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO5017837.1"
                     /translation="SITFHIWTAYSPFTTWVQIVYDWLDALKDPNGLKTFVNTTLGET
                     WEEAVGEKLDHQVLMDKVVRYTAAVPSRVVYLTAGIDSQRNRFEMYVWGWAPGEEAFL
                     VDKIIIMGRPDEEETLLRVDVAINKKYRHADGTEMTIS"
BASE COUNT          102 a           99 c          126 g           93 t
ORIGIN      
        1 ctccatcaca ttccatatct ggacggcgta cagtccgttc accacctggg tacagattgt
       61 ctatgactgg ctggatgcac tgaaagatcc caacggcctg aaaacctttg tgaacaccac
      121 gctgggcgag acctgggaag aggccgtggg cgaaaaactc gatcaccagg tgctgatgga
      181 taaggttgtg cgttacacgg ctgcggtgcc ttcccgggtg gtttatctga cggcgggcat
      241 tgactcgcag cgaaaccgtt ttgagatgta tgtctgggga tgggctccgg gagaggaagc
      301 ctttctggtg gataaaatca tcattatggg gcgtcccgat gaggaagaga cgctgttacg
      361 tgtggatgtg gcgatcaaca aaaaataccg ccatgcagac ggaaccgaaa tgaccatttc
//
LOCUS       DAZFFE010000362          414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZFFE010000362 DAZFFE010000000
VERSION     DAZFFE010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.365"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>414
                     /locus_tag="ACHJ69_005073"
     CDS             <1..>414
                     /locus_tag="ACHJ69_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO5017838.1"
                     /translation="YQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGT
                     DTAKEILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEELVEKWEKGKMR
                     LLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAV"
BASE COUNT          104 a           96 c          126 g           88 t
ORIGIN      
        1 tatcagaggt caaaaaaaca cggtgttttc cgggtgctgc cggtaaaagg tgcatctgtt
       61 tatggcaagc cggtgatcac catgccaaaa acccgcaatc agcggggcgt gtatctgtgc
      121 gaagtgggga cggacaccgc aaaagaaatt ctctatgccc gtatgaaagc cgatcccacg
      181 cctgcggatg aagccacgtc gtatgccatc cgttttcctg atgatccgga gattttttcg
      241 cagacagagg cgcagcaact ggtcgcggaa gagcttgtgg agaagtggga aaaaggaaag
      301 atgcgtctgc tgtgggataa caaaaagcgg cgtaacgaag cgctggactg cctggtgtat
      361 gcctacgcgg cattacgtgt gtccgtgcaa cgctggcagc ttgatctggc tgta
//
LOCUS       DAZFFE010000363          405 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFFE010000363 DAZFFE010000000
VERSION     DAZFFE010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.366"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          138 a           71 c           78 g          118 t
ORIGIN      
        1 atctgtattc aatgattcgc tcaaagattg aaattcaaga gaaattgtta tagcagtgat
       61 acgaaacaac aaaacggaat gattgccagc cgttagaaaa caccggacac tctcgtgtaa
      121 aaagctaccc tatctatgga tatagcgata tcaactcatc aagcgtggac tgaatgatat
      181 tcagctccat atgggcggca acctggagga taaacatgat ttcgccgttg gaatcaaggt
      241 tgttctgcaa tatctaccag actgcttcaa tttatctgtt ttggcagtgc cataatctta
      301 atttaactat ttgaagatga tgatattgta agatgccaga aatttagtaa tctggagaac
      361 catgtttacg atcactaaat attaaagtaa tagtcgataa ttcag
//
LOCUS       DAZFFE010000364          398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFFE010000364 DAZFFE010000000
VERSION     DAZFFE010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.367"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          145 a           69 c           52 g          132 t
ORIGIN      
        1 tcggcttcat agtagcgttg ggtcaggtcg tccattcaga gagttatttc ccatttaact
       61 aaaaagttag ataacagtaa aaagaaaata tattttacag ttcataaaac accagtaaaa
      121 aacaacaata acccataaag atgagcgcaa ttaaaaaacc attacaatca tctgttaaaa
      181 ataaacaatg actactttgc ttatagtatt gccggtcagt tacttcatct tcctgggtat
      241 tacatcttta atatagtgtg tgatatttac atttcgtttt acatgacatt gatttatttg
      301 ccatgtaaga ttaagcaatc tcacactgaa aataaaatta actgttactg atacatcgct
      361 actctattgt tttttagata aaacatcata acctcatc
//
LOCUS       DAZFFE010000365          395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFFE010000365 DAZFFE010000000
VERSION     DAZFFE010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.368"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>395
                     /locus_tag="ACHJ69_005074"
     CDS             <1..>395
                     /locus_tag="ACHJ69_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5017839.1"
                     /translation="AIRENTDGTFAVTAVQHVPEKEAIVDNGATFEPQSGTLNSVIPP
                     AVQHLTVEVSAADSQYLALAKWDTPRVVKGVRFSLRLTSGSGENSRLLTTAITAGTEH
                     RFSGLPLGEYTLTVRAINSYGQQGEPATTT"
BASE COUNT           84 a          124 c          128 g           59 t
ORIGIN      
        1 gccatccggg aaaacacgga cggcaccttt gccgttacgg cggtgcagca cgtaccggaa
       61 aaagaggcca tcgtggataa cggggccacc tttgagccgc agtcaggcac cctgaacagc
      121 gtcatccctc cggcagtgca gcacctcacg gtggaggtga gcgcggctga cagtcagtat
      181 ctggcgctgg cgaaatggga cacgccgagg gtggtgaagg gcgtgcgctt cagtctgcgc
      241 ctgaccagtg gaagcggtga gaacagccgc ctgctgacca ccgccatcac tgccggtacg
      301 gagcaccgtt tcagtggcct gcctctcggg gaatacaccc tgacggtcag ggcgattaac
      361 agctatggcc agcagggcga acccgccacc accac
//
LOCUS       DAZFFE010000366          393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFFE010000366 DAZFFE010000000
VERSION     DAZFFE010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.369"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..75
                     /locus_tag="ACHJ69_005075"
     CDS             <1..75
                     /locus_tag="ACHJ69_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO5017840.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(91..393)
                     /locus_tag="ACHJ69_005076"
                     /pseudo
     CDS             complement(91..393)
                     /locus_tag="ACHJ69_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
BASE COUNT          103 a          106 c           92 g           92 t
ORIGIN      
        1 ctgtcgttct caaaatcggt ggagctgcat gacaaggtca tcgggcatta tctgaacata
       61 aaacactatc agtaagttgg agtcattacc caaaatcgtc tcttccgtca aaatccgtcg
      121 cgcgcctaaa aaatggtcct gccagaaagg ttcggctaac cgacttaccc gaatggtttc
      181 cccggtgcgc ggtgactcga taaactgacc gtctccaaga tatacgccca tatggtcggc
      241 tatctcacga ctgtggatat ggaaaaacag caaatctccc cggcgcaggt cgttattcgc
      301 cacaatcgtt gcccgacggt agtggtacat ctcattggcc gtgcgcggga gcttagcctc
      361 aaggatcttg ttgtaggcat aaaaaaccaa ccc
//
LOCUS       DAZFFE010000367          385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFFE010000367 DAZFFE010000000
VERSION     DAZFFE010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.370"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..354
                     /locus_tag="ACHJ69_005077"
     CDS             <1..354
                     /locus_tag="ACHJ69_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO5017841.1"
                     /translation="EHLALLKRRAEEAEATGNLQVSVEDLVRTGAFLVAMSLWHNHPQ
                     KTQSPSMNEAVMKIEQEVLTTWPADAIARAEDVVLCLSGMIEAVRPDTDITEVAKNNA
                     LTDDDFSAGKPSTAS"
BASE COUNT           90 a           93 c          120 g           82 t
ORIGIN      
        1 gagcatcttg ccctcctgaa acggcgggca gaagaggctg aagccaccgg caacctgcag
       61 gtgagtgtgg aagatcttgt cagaaccggc gcgtttctgg tggcgatgtc cctgtggcat
      121 aaccatccac agaaaacgca gtcaccatca atgaatgagg ccgtgatgaa gatagagcag
      181 gaagtgctca ccacctggcc tgccgatgcc attgcccggg cggaagacgt ggtgttgtgc
      241 ctgtccggga tgatcgaagc tgttcgtccg gatactgata ttactgaagt ggcgaaaaat
      301 aacgcgctta ctgatgatga tttttctgcg ggaaagcctt cgacggcgag ctgaattttg
      361 ccctcagact ggcgcgtgag atggg
//
LOCUS       DAZFFE010000368          385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFFE010000368 DAZFFE010000000
VERSION     DAZFFE010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.371"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..354
                     /locus_tag="ACHJ69_005078"
     CDS             <1..354
                     /locus_tag="ACHJ69_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO5017842.1"
                     /translation="EHLALLKRRAEEAEASGNLQVSVEDLVRTGAFLVAMSLWHNHPE
                     KVQLLSMNEAVMQIEQDVLTTWPADAIARAEEVVLRLSGMSGAVHANTDSTEVAKNNT
                     LTDDDFSAGKSSTAS"
BASE COUNT           86 a           88 c          132 g           79 t
ORIGIN      
        1 gagcatcttg ccctgctgaa acggcgggca gaagaggctg aagccagcgg caacctgcag
       61 gtgagtgtgg aagatcttgt cagaaccggc gcgtttctgg tggcgatgtc cctgtggcat
      121 aaccatccgg agaaagtgca gctgctgtca atgaatgagg ccgtgatgca gatcgaacag
      181 gatgtgctga cgacctggcc ggctgatgcc attgcccggg cggaagaggt ggtgttacgt
      241 ctgtccggga tgagcggggc tgttcatgcg aatactgaca gcaccgaagt ggcgaaaaat
      301 aacacgctga ctgatgatga tttttctgcg ggaaagtctt cgacggcgag ctgaactttg
      361 ccctcagact ggcgcgtgag atggg
//
LOCUS       DAZFFE010000369          374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZFFE010000369 DAZFFE010000000
VERSION     DAZFFE010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.372"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            264..>374
                     /locus_tag="ACHJ69_005079"
     CDS             264..>374
                     /locus_tag="ACHJ69_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO5017843.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTT"
BASE COUNT           88 a           92 c           90 g          104 t
ORIGIN      
        1 agcgaagagc ccagtcggaa gactgggctt ttttgcgttg gtgcggaata aatgccgcat
       61 cttatccgac cttgtattat ccctccagtg cagagaaaat cggccagttt tctctgcctg
      121 cagtccgcat gccgtatccg gccttgggtt ctaacctgca tggtagattt atgcggcgga
      181 ctgcctttct cccgaagtga taaaccggac agtatcatag accggttttc ctggtaatcc
      241 gtatttgcaa ggttggtttc actatggaac atgaacttca ttatatcggt atcgacaccg
      301 ctaaagagaa actggatgtc gatgtgttgc gtcctgatgg tcgtcatcgc accaaaaaat
      361 tcgctaacac cact
//
LOCUS       DAZFFE010000370          356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZFFE010000370 DAZFFE010000000
VERSION     DAZFFE010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.373"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..294
                     /locus_tag="ACHJ69_005080"
     CDS             <1..294
                     /locus_tag="ACHJ69_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO5017844.1"
                     /translation="NDPAHTATGSSGISQATPALTPLMLDEASGKLVVWDGQKAGSAA
                     GILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKANAFVGTALSHAALP"
BASE COUNT           85 a           93 c          112 g           66 t
ORIGIN      
        1 aatgatccgg ctcataccgc cacaggcagc agcgggattt ctcaggcaac accggcactg
       61 acgcccctga tgctggatga agccagcggg aaactggtgg tctgggacgg acagaaagcc
      121 ggtagtgcgg ctggcatact ggtactgccg cttgaaggca cagagacggt gctgacgtat
      181 tacaagtcgg ggacctttgc gacggaggca atccgctggc ctgaaagtgt ggatgaacac
      241 aaaaaggcca acgcctttgt cggcacagcc ctgagtcacg cggcgctgcc gtaacacgtt
      301 atcaggccac cgcgttggcc tgactgattt ctgaatgaaa ggaactgatt tatggg
//
LOCUS       DAZFFE010000371          353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFFE010000371 DAZFFE010000000
VERSION     DAZFFE010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.374"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..159
                     /locus_tag="ACHJ69_005081"
     CDS             <1..159
                     /locus_tag="ACHJ69_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO5017845.1"
                     /translation="DRERINAARDKLRAETDILTSQRRGVVTPVSDIVSSLHEMSNSG
                     RLDDIPEE"
     gene            140..>353
                     /locus_tag="ACHJ69_005082"
     CDS             140..>353
                     /locus_tag="ACHJ69_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO5017846.1"
                     /translation="MTFRKNEPRCDEPSEMTGTEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRSMHNKNII"
BASE COUNT          106 a           85 c           93 g           69 t
ORIGIN      
        1 gaccgggaaa gaatcaacgc tgccagagat aaactcagag cggaaaccga tattctgacc
       61 agccagcgtc ggggcgttgt tacgcctgtc agtgacatcg tgtcatcgct gcatgaaatg
      121 agtaattcgg ggagactgga tgacattccg gaagaatgaa ccgcgatgtg atgaaccgtc
      181 agaaatgacc gggaccgaac aacgtctgtt catcatgact aaactgagca atccctggtg
      241 gcggctcaat catctctaca agatacagaa cgaaaaaggt gagctggtca ccttcagaat
      301 gcgaccggcg cagcgccagt tgttccggag catgcacaat aaaaatatta tcc
//
LOCUS       DAZFFE010000372          347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFFE010000372 DAZFFE010000000
VERSION     DAZFFE010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.375"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..270
                     /locus_tag="ACHJ69_005083"
     CDS             <1..270
                     /locus_tag="ACHJ69_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HFO5017847.1"
                     /translation="PVSGVTIKPETLTLEKGKAGTLTVAVLPDNASDPSLRVSTVHRA
                     VATATLKENTVTVNALEKGTTSVVVMSDNGNFVALATVTVNVPAA"
BASE COUNT           90 a           77 c           97 g           83 t
ORIGIN      
        1 ccggtttccg gtgtgaccat taaaccggaa accctgaccc tagaaaaagg gaaggcaggc
       61 acacttactg tggctgtgtt gccggataat gcgtctgatc cttcattaag ggtgtcgacc
      121 gtgcatcgtg cggtggccac agccacgttg aaggagaaca cagtgacggt aaacgcactt
      181 gagaaaggaa caacgtcggt cgtggtcatg agtgataacg gtaattttgt cgctctggcg
      241 acagtaaccg ttaatgtccc tgccgcgtaa cgggtgttta ggagaacacg catgtttctt
      301 aaaacagaac aatttgaata taacggtgtg tctgtcacgc tttcgga
//
LOCUS       DAZFFE010000373          338 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZFFE010000373 DAZFFE010000000
VERSION     DAZFFE010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.376"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>338)
                     /locus_tag="ACHJ69_005084"
     CDS             complement(<1..>338)
                     /locus_tag="ACHJ69_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5017848.1"
                     /translation="GDSTVSLGFARIRFPGLKDFVRDAGVYNRDTFRNVVNVNHFNSS
                     AEYENAIARGHDGTAKSPLGMSIRYRYEITDELGVIASFTWARSMTNAQAFIDVKPSD
                     PSREVKNPAA"
BASE COUNT           85 a           89 c           76 g           88 t
ORIGIN      
        1 agctgccggg ttttttacct ctcgtgatgg atcggaaggc ttaacatcaa taaatgcctg
       61 cgcattagtc atggagcgcg cccatgtaaa agaggctata acacccagtt catcagttat
      121 ctcgtaacga tacctgatgc tcatccccag ggggcttttg gctgtgccgt catgcccccg
      181 ggcaatagca ttttcgtatt cagctgatga attaaaatgg tttacgttta caacattcct
      241 gaacgtatcc cggttataaa cacccgcgtc tctaacaaaa tccttcagcc cggggaagcg
      301 tatgcgtgca aacccgagag ataccgtact atcgcctg
//
LOCUS       DAZFFE010000374          338 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZFFE010000374 DAZFFE010000000
VERSION     DAZFFE010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.377"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..122
                     /locus_tag="ACHJ69_005085"
     CDS             <1..122
                     /locus_tag="ACHJ69_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO5017849.1"
                     /translation="LCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            119..>338
                     /locus_tag="ACHJ69_005086"
     CDS             119..>338
                     /locus_tag="ACHJ69_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO5017850.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEV"
BASE COUNT           88 a           91 c           93 g           66 t
ORIGIN      
        1 cgctctgttc ccagatattt cgccgcgaca ttcgcgcatc gtcagaactg acacaggccg
       61 aagcagtgaa agctcttgga ttcctgaaac agaaagccac tgagcagaag gtggcagcat
      121 gacaccggac attatcctgc agcgtaccgg gatcgacgtg agagctgtcg aacaggggga
      181 tgatgcatgg cacaaattac ggctcggcgt catcaccgct tcagaagttc acaacgtgat
      241 agcaaagccc cgctcaggaa agaagtggcc tgacatgaaa atgtcctact tccacaccct
      301 gctggctgag gtttgcaccg gtgtggctcc ggaagtta
//
LOCUS       DAZFFE010000375          328 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZFFE010000375 DAZFFE010000000
VERSION     DAZFFE010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.378"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            49..>328
                     /locus_tag="ACHJ69_005087"
     rRNA            49..>328
                     /locus_tag="ACHJ69_005087"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           97 a           71 c           96 g           64 t
ORIGIN      
        1 attttcgcaa cacgatgatg gatcgcaaga aacatcttcg ggttgtgagg ttaagcgact
       61 aagcgtacac ggtggatgcc ctggcagtca gaggcgatga aggacgtgct aatctgcgat
      121 aagcgtcggt gaggtgatat gaaccgttat aaccggcgat ttccgaatgg ggaaacccag
      181 tgtgtttcga cacactatca ttaactgaat ccataggtta atgaggcgaa ccgggggaac
      241 tgaaacatct aagtaccccg aggaaaagaa atcaaccgag attcccccag tagcggcgag
      301 cgaacgggga ggagcccaga gcctgaat
//
LOCUS       DAZFFE010000376          327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZFFE010000376 DAZFFE010000000
VERSION     DAZFFE010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.379"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>327
                     /locus_tag="ACHJ69_005088"
     CDS             <1..>327
                     /locus_tag="ACHJ69_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5017851.1"
                     /translation="TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPES
                     SADMLASAQKAFDEADKKWQWYQSRSQRRGKTSSFRANLQGAWDDRENARLGLAAATL
                     QSDMEKA"
BASE COUNT           88 a           79 c          104 g           56 t
ORIGIN      
        1 acgaaaggct ttgatgagca gacccgtcgc ctgaaagaaa acatgggaac actggagacc
       61 tgggcggata aaacagggaa ggcattcaaa tcgatgtggg atgccattct ggatatcggt
      121 cgtccggaat cctcagcgga tatgctcgcc agtgcgcaga aggcatttga tgaggcggat
      181 aaaaaatggc agtggtacca gagccggagc cagcgccgcg gtaaaacctc ctctttccgg
      241 gccaaccttc agggcgcatg ggatgaccgg gaaaatgccc gtctgggtct ggcagcggca
      301 acgctgcagt cggatatgga aaaagcc
//
LOCUS       DAZFFE010000377          326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFFE010000377 DAZFFE010000000
VERSION     DAZFFE010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.380"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          131 a           46 c           49 g          100 t
ORIGIN      
        1 aaaatctgga gcgggctatc agatgatgaa atgaaaacat ttgaactcgc caacaaaaaa
       61 ttattatcaa accttaatgt taattgatgt taattgatgt taattgatgt taaaacatac
      121 taacagtcat cttcttacaa tgtattcgtc tggagagaat aactaccaga atatataaag
      181 acattaatca cactctcatt taattacaat gagcataaga gccaccaatg tttatgctgg
      241 ttaggcttta tataggaaac aataaaaaaa cgtcattaaa caaagcgttt ttaatggcgt
      301 tgttatttaa aagaaagata tcaaat
//
LOCUS       DAZFFE010000378          326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFFE010000378 DAZFFE010000000
VERSION     DAZFFE010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.381"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..236)
                     /locus_tag="ACHJ69_005089"
     CDS             complement(<1..236)
                     /locus_tag="ACHJ69_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5017852.1"
                     /translation="MTDIELTGKSITLTDKYGSCVHQNIVLTYLMELYQMDFAELSEA
                     VSTHYPSHKGVIMTIAEQLEEKGLEKGRAEERSR"
BASE COUNT           85 a           82 c           72 g           87 t
ORIGIN      
        1 aatctggagc gctcttcagc acgacctttc tccagacctt tctcttccag ttgctctgct
       61 atcgtcatga tcaccccctt gtggctggga tagtgtgtgg atactgcttc agaaagttca
      121 gcaaaatcca tctggtaaag ttccatcaga taagtcagca cgatattctg gtgaacgcaa
      181 ctgccatact tatccgtcag tgtgatggat ttaccagtca gctcaatatc tgtcactgcc
      241 agaaactacc aaaaaaacgc ctgcacaagg caggcgtggt gtaaaaaggg aacagcgtat
      301 tccggttatt cagtcctggt taagcg
//
LOCUS       DAZFFE010000379          325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFFE010000379 DAZFFE010000000
VERSION     DAZFFE010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.382"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..303
                     /locus_tag="ACHJ69_005090"
     CDS             <1..303
                     /locus_tag="ACHJ69_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5017853.1"
                     /translation="GEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAE
                     GINFNQNNHVVIQNDGPNGRAGPQLMKAVYEMARKGAQDELRLQLRDGGMLSGSGG"
BASE COUNT           72 a           68 c          123 g           62 t
ORIGIN      
        1 ggggagtttg tcttcacgaa ggaggcgacc agccggattg gtgtcggcaa tctgtaccgc
       61 ctgatgcggg gctatgcgga aggtggttat gtcggcggtg ccggaagtcc ggcgcagatg
      121 cggcgggcgg aaggcattaa ttttaatcag aacaatcacg tggtgattca gaacgacggc
      181 cccaacgggc gggcagggcc gcagctgatg aaagcggtgt atgagatggc ccgcaagggg
      241 gcacaggatg aactccggct gcagttgcgt gatggcggta tgttatcagg gagcggtgga
      301 tgaaaacctt tcgctggaaa gtgaa
//
LOCUS       DAZFFE010000380          323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZFFE010000380 DAZFFE010000000
VERSION     DAZFFE010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.383"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>323)
                     /locus_tag="ACHJ69_005091"
     CDS             complement(<1..>323)
                     /locus_tag="ACHJ69_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFO5017854.1"
                     /translation="SSINMPCTLFETITLFDDFSADDMQYGDMEEQDFLSLGLSDISA
                     KVDPYRLIKYHFPGPNSTYGAFSVPTSGTKISQSECIDILFAEMKDLAKMFSFFGQYK
                     TLIQDL"
BASE COUNT          113 a           52 c           62 g           96 t
ORIGIN      
        1 agatcctgga tgagtgtttt gtattgtcca aaaaaggaaa acatttttgc cagatctttc
       61 atttctgcaa ataaaatatc aatacattca ctttgtgata tttttgttcc agaagttgga
      121 acactaaaag caccataagt agaattcggc ccaggaaaat ggtatttaat gaggcggtaa
      181 ggatcaactt ttgcagagat gtcgcttaag ccgagagaga gaaaatcttg ttcttccata
      241 tccccatact gcatatcatc agcagaaaaa tcatcgaata aagtaatagt ttcgaaaagt
      301 gtacagggca tattaattga tga
//
LOCUS       DAZFFE010000381          323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZFFE010000381 DAZFFE010000000
VERSION     DAZFFE010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.384"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>323)
                     /locus_tag="ACHJ69_005092"
     CDS             complement(<1..>323)
                     /locus_tag="ACHJ69_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO5017855.1"
                     /translation="VDNGARFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLALAKWDT
                     PRVVKGVRFSLRLTSGSSENSRLLTTAITAGTEHRFSGLPPGEYTLTVRAINSYGQQG
                     EPATTT"
BASE COUNT           48 a          108 c          102 g           65 t
ORIGIN      
        1 gtggtggtgg ccggttcgcc ctgctggcca tagctgttaa tcgccctgac cgtcagggtg
       61 tattccccgg gaggcaggcc actgaaacgg tgctccgtac cggcagtgat ggcggtggtc
      121 agcaggcggc tgttctcact gcttccactg gtcaggcgca gactgaagcg cacgcccttc
      181 accacccgcg gcgtgtccca tttcgccagc gccagatact ggccgtcagc tgcgctcacc
      241 tccaccgtca ggtgctgcac tgccggtggg ataacgctgt tcagggtgcc tgactgcggc
      301 tcaaagcggg ccccgttatc cac
//
LOCUS       DAZFFE010000382          321 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZFFE010000382 DAZFFE010000000
VERSION     DAZFFE010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.385"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..214)
                     /locus_tag="ACHJ69_005093"
     CDS             complement(<1..214)
                     /locus_tag="ACHJ69_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017856.1"
                     /translation="MKVILATRNRYLEYGLQALLKEHSVIVAREFFLPENRRYIPDFD
                     ESWLIICDVLLGRLMRCMFQGRHFLQL"
BASE COUNT           87 a           96 c           63 g           75 t
ORIGIN      
        1 ccagttgcag aaaatgtcgt ccctgaaaca tacagcgcat cagcctgccc agtaaaacat
       61 cacagattat cagccaggat tcatcaaagt ccgggatata acggcggttc tccggcagga
      121 aaaactccct cgccactatc acactgtgtt ccttcagcaa cgcctgcagg ccatattcca
      181 gatagcggtt ccgggttgcc agtatcacct tcatcgcagc accttcctgt ttttatgcct
      241 catgaaaaaa acggcaggca aaactcctgt gcctgccgtc atgcttcatg tcactgtcag
      301 caattaaaac tcgaagttca c
//
LOCUS       DAZFFE010000383          315 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZFFE010000383 DAZFFE010000000
VERSION     DAZFFE010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.386"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>315
                     /locus_tag="ACHJ69_005094"
     CDS             <1..>315
                     /locus_tag="ACHJ69_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO5017857.1"
                     /translation="GFTAGAEGAFNEASGATAGSSLWGADKPLYHDLVSRTRAALKKN
                     PKNVLLSVIWMQGEKDVSSGRHAEHNALFLAMVNQFRTELADVAEQCTGGTTASVPWI
                     CGD"
BASE COUNT           74 a           71 c          108 g           62 t
ORIGIN      
        1 ggatttactg cgggggccga gggggcgttt aacgaagcgt cgggagcgac cgcaggctcg
       61 tccctgtggg gggcggataa accgctgtat catgacctgg tcagcagaac ccgtgcggcc
      121 ctgaagaaaa atccgaagaa cgtgctgtta tcagtgatct ggatgcaggg agaaaaagat
      181 gtcagttcgg ggagacatgc agaacacaat gcgcttttcc ttgccatggt aaatcagttc
      241 cgtacggaac tggcagatgt ggcagagcag tgtactggcg ggacaacggc cagcgtcccg
      301 tggatttgcg gtgat
//
LOCUS       DAZFFE010000384          314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZFFE010000384 DAZFFE010000000
VERSION     DAZFFE010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.387"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          102 a           50 c           59 g          103 t
ORIGIN      
        1 aaacagttaa gcacaatttg attatttgac tttcttcttt ttgtgactaa cttcacaatt
       61 aaagatgtaa ccgtatacaa attgttgatg aataaagaga cagcataagt ggcaggccag
      121 tcgtaatgcg gatatccctt cgcggacgtg atatcaccga cgttatagta aggaaattcc
      181 gaaaaacttt atattaaaat tcggaatttc ttcaggagta agctcatgag agcggcgaga
      241 catcagattt tctttatata tccgatgttg aactgtttta tttgtataca gataatttta
      301 ttagggtata ccac
//
LOCUS       DAZFFE010000385          299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZFFE010000385 DAZFFE010000000
VERSION     DAZFFE010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.388"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            27..>299
                     /locus_tag="ACHJ69_005095"
     CDS             27..>299
                     /locus_tag="ACHJ69_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO5017858.1"
                     /translation="MSRKLHYGLSVAVLALIATGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVVPGMKLSKEKCAQVNAIERDKALAWVEK"
BASE COUNT           74 a           68 c           93 g           64 t
ORIGIN      
        1 caaagctgcg cggggagagt aagctgatga gcaggaaact ccactatggt ttatcggtag
       61 ccgttctggc gctgattgcc acaggtgctt ctgcgcctga aatcctcgac cagtttcttg
      121 acgaaaaaga aggtaaccac accacggcat accgtgatgg tgcggggatc tggaccatct
      181 gccgtggtgc catcatggtg gatggcaaac ctgtcgtccc gggcatgaag ttgtcgaagg
      241 aaaaatgcgc tcaggttaat gccattgaac gtgataaggc gctggcatgg gtggagaaa
//
LOCUS       DAZFFE010000386          292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZFFE010000386 DAZFFE010000000
VERSION     DAZFFE010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.389"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(61..>292)
                     /locus_tag="ACHJ69_005096"
     CDS             complement(61..>292)
                     /locus_tag="ACHJ69_005096"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017859.1"
                     /translation="QARPDPGVKKVIQDLQVRRDQLARQAHRGLPEGTAMFPPGNMRL
                     VLMFLACPLSMLTAKAYFHQGYMKTFRVAPLI"
BASE COUNT           65 a           82 c           71 g           74 t
ORIGIN      
        1 gtttcaaaaa ccagatttga tttggtgttg atgcgcctgt ttgtgttgtc tgtattgaaa
       61 tcagatcaat ggagctaccc tgaatgtttt catatatccc tgatgaaaat aagccttcgc
      121 cgtcagcata gacagaggac acgccaaaaa cataagaacc aaccgcatat ttcccggtgg
      181 aaacattgcc gtcccttccg gcaaccccct gtgggcctgc cgggccagct ggtccctgcg
      241 gacctgcagg tcctgtatca cctttttgac cccggggtcc ggccgggcct gc
//
LOCUS       DAZFFE010000387          291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFFE010000387 DAZFFE010000000
VERSION     DAZFFE010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.390"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..146
                     /locus_tag="ACHJ69_005097"
     CDS             <1..146
                     /locus_tag="ACHJ69_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO5017860.1"
                     /translation="YRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQ
                     MTA"
BASE COUNT           92 a           62 c           62 g           75 t
ORIGIN      
        1 actatcgttt taataaccgg gatgaagcca tctcagtaat acgggaatac attgagattt
       61 tctacaatcg tcagcgtcgt cactctcgtc tggggaatat ctccccggca gccttcaggg
      121 aaaaatatca tcagatgact gcttaaaaaa agaacaaatg gtagtgtccg ctattgccag
      181 tacacctcat attcagtgat gcctgtcaca cgctccggaa cgcggatggt agcggcatat
      241 cagaaaacgg ttaatattat taatgtatcg acaaaaaatc aaaatgtggt g
//
LOCUS       DAZFFE010000388          286 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFFE010000388 DAZFFE010000000
VERSION     DAZFFE010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.391"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>286
                     /locus_tag="ACHJ69_005098"
     CDS             <1..>286
                     /locus_tag="ACHJ69_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HFO5017861.1"
                     /translation="VDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNG
                     KLRPLGIPALRDRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTV"
BASE COUNT           64 a           81 c           80 g           61 t
ORIGIN      
        1 gcgttgatgg cgtgaacaaa acaatgctac aggccagact ggctgttgag ctgcaaatcc
       61 tcagggatga attactctca ggccactacc agcccttgcc cgccagacgg gtttacatcc
      121 ctaaaagcaa cggcaaactg cgaccactgg gtatccccgc gttgcgggat cgtattgttc
      181 agcgggccat gctgatggcg atggagccga tatgggagag tgattttcat acgctctcat
      241 atggcttccg gcctgagcgc agtgtccacc acgcgatccg cacggt
//
LOCUS       DAZFFE010000389          285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFFE010000389 DAZFFE010000000
VERSION     DAZFFE010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.392"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>285)
                     /locus_tag="ACHJ69_005099"
     CDS             complement(<1..>285)
                     /locus_tag="ACHJ69_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5017862.1"
                     /translation="SDRWVVEQMSQLTAMTASFVLATPTETDGALFPGRIMLANTCMW
                     TYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNVGNFGGFLS"
BASE COUNT           62 a           97 c           72 g           54 t
ORIGIN      
        1 ggaaaggaaa ccgccaaaat tgccgacatt cctgcgcagt tcacacccgc gcatgcactt
       61 gctgcatctg tccttacgga tatccgtggt gggtttatcg aactcatccg ccacagcccc
      121 gcccgtgtaa ccacactcat cagagcggta ggtccacata caggtgttcg ccagcatgat
      181 acgaccggga aacagcgccc cgtccgtctc ggtcggtgtg gccagcacaa acgaggccgt
      241 catggctgtc agctgcgaca tctgctccac cacccagcgg tcact
//
LOCUS       DAZFFE010000390          284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFFE010000390 DAZFFE010000000
VERSION     DAZFFE010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.393"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>284
                     /locus_tag="ACHJ69_005100"
     CDS             <1..>284
                     /locus_tag="ACHJ69_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO5017863.1"
                     /translation="QRDDWLKKEILRVYDGNHQVYGVRKVWRQLLREGIRVARCTVAR
                     LMAVMGLAGVLRGKKVRTTVSRKAVAAGDRVNRQFVAERPDQLWVADFTY"
BASE COUNT           63 a           64 c           94 g           63 t
ORIGIN      
        1 cgcagcgcga tgactggctg aagaaagaga tactgcgcgt atacgatggg aatcatcagg
       61 tatacggtgt gcgtaaagtc tggcgtcagt tgttacggga aggaatcagg gtagccagat
      121 gtacagtggc gcgcctcatg gcggttatgg gacttgccgg tgttctccgg ggtaaaaagg
      181 tccgcactac cgtcagccgg aaagccgttg ccgcaggcga ccgcgtaaac cgtcagtttg
      241 tggcagaacg tcctgaccag ttgtgggtgg ctgattttac ttac
//
LOCUS       DAZFFE010000391          283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFFE010000391 DAZFFE010000000
VERSION     DAZFFE010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.394"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT           84 a           57 c           59 g           83 t
ORIGIN      
        1 gcaaagtggc aattacgcat agtttcccga taaagacgcg atagcgacat cccgcataag
       61 gcatttttct ctttatcttt gtacggtact tcatggaaca gagtttttga ccttgcgaat
      121 cgtgatgtct gttggggggc aacttgctca ctgaagcgtg agactcgatt aagcgcacga
      181 aacacagaaa tcaaaaaacc cggtcacttt tttacaaggt aaccgggtaa aaataacttt
      241 tattttttaa ctgttttgag actcatagag atgtctcaaa act
//
LOCUS       DAZFFE010000392          278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFFE010000392 DAZFFE010000000
VERSION     DAZFFE010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.395"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            24..100
                     /locus_tag="ACHJ69_005101"
     tRNA            24..100
                     /locus_tag="ACHJ69_005101"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ACHJ69_005102"
     tRNA            143..218
                     /locus_tag="ACHJ69_005102"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           75 a           63 c           72 g           68 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacc gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaag
//
LOCUS       DAZFFE010000393          275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFFE010000393 DAZFFE010000000
VERSION     DAZFFE010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.396"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>275
                     /locus_tag="ACHJ69_005103"
     CDS             <1..>275
                     /locus_tag="ACHJ69_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO5017864.1"
                     /translation="FGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAP
                     FVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV"
BASE COUNT           49 a           84 c           83 g           59 t
ORIGIN      
        1 ttcggcgtga cctacaccgg taactatgag ttcccgcagc cgctgcacga tgcgctggat
       61 aaattccagg ccgacaccgg tatcgacatc gacatgcaca tcgacgccgc cagcggtggc
      121 ttcctggcac cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa
      181 tcgatcagtg cttcaggcca taaattcggt ctggctccgc tgggctgcgg ctgggttatc
      241 tggcgtgatg aagaagcgct gccgcaggaa ctggt
//
LOCUS       DAZFFE010000394          266 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFFE010000394 DAZFFE010000000
VERSION     DAZFFE010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.397"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(69..>266)
                     /locus_tag="ACHJ69_005104"
     CDS             complement(69..>266)
                     /locus_tag="ACHJ69_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017865.1"
                     /translation="FPGMTVEAARDILSSSPDKAEPVSASTTAMFERIMANHSPAAVQ
                     GGVSQTSADGDADVKMLMAMP"
BASE COUNT           56 a           70 c           72 g           68 t
ORIGIN      
        1 gcacgttgtt cagtgatggt tttcgttacc atattgtaaa aacctcatat tgatggtcag
       61 cactgacttc atggcatggc catgagcatt ttcacgtccg catcaccgtc tgccgacgtc
      121 tgtgacacgc caccctgcac ggctgccggt gaatggttcg ccatgatgcg ttcaaacatg
      181 gcggttgtgg atgcagaaac cggttctgct ttatccggcg atgaggagag aatgtcgcgg
      241 gcagcctcca ccgtcattcc gggaaa
//
LOCUS       DAZFFE010000395          266 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFFE010000395 DAZFFE010000000
VERSION     DAZFFE010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.398"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..198
                     /locus_tag="ACHJ69_005105"
     CDS             <1..198
                     /locus_tag="ACHJ69_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO5017866.1"
                     /translation="CPGMTVENARAVLAAGSGKAEPVSASTTALFEHFMANHSPAAVR
                     GGVSQTSADGDADVKMLMAMP"
BASE COUNT           67 a           67 c           80 g           52 t
ORIGIN      
        1 tgcccgggaa tgaccgtgga gaacgcccgg gctgtgctgg ctgcgggatc aggtaaggcc
       61 gaaccggtat ctgcatccac aaccgccctg tttgaacatt tcatggcgaa tcattcaccg
      121 gcagcggtgc ggggtggcgt gtcacagacg tcagcagacg gtgatgcgga cgtgaaaatg
      181 ctcatggcca tgccatgaag tcagtgctga ccatcaatat gaggttttaa caaaatggtg
      241 acgaaaacca tcactgaaca acgtgc
//
LOCUS       DAZFFE010000396          264 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZFFE010000396 DAZFFE010000000
VERSION     DAZFFE010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.399"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>264
                     /locus_tag="ACHJ69_005106"
     CDS             <1..>264
                     /locus_tag="ACHJ69_005106"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017867.1"
                     /translation="LHKEKQIINQKIKLIPFVEKEGILNKKEDITSNYFWFDRSLKNS
                     IFNIEGVFVNTSICFDALFPQIALDNTNLTIIQSNYSLLNKGYG"
BASE COUNT          105 a           38 c           35 g           86 t
ORIGIN      
        1 cttcataagg aaaaacaaat aattaatcaa aaaataaaac ttatcccatt tgtcgaaaag
       61 gaaggcattc taaataaaaa agaggatatt acatctaatt atttctggtt cgatagaagt
      121 ttgaaaaaca gtatatttaa tatagaaggt gtatttgtca acacttcgat ttgttttgat
      181 gctcttttcc cacaaatagc actcgacaat actaatctga ctataattca atctaactat
      241 agtttactta ataaaggata tggc
//
LOCUS       DAZFFE010000397          261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZFFE010000397 DAZFFE010000000
VERSION     DAZFFE010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.400"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            99..>261
                     /locus_tag="ACHJ69_005107"
     CDS             99..>261
                     /locus_tag="ACHJ69_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HFO5017868.1"
                     /translation="MKKILYVTGSRAEYGIVRRLLTMLRETPEIQLDLAVTGMHCDNA
                     YGNTIHIIEQ"
BASE COUNT          103 a           29 c           52 g           77 t
ORIGIN      
        1 tcttatggca atctaaataa aatgtatact tatgatggct tacattttaa tagtaatggg
       61 tatacagtat tagaaaacga aatagcggag attgttaaat gaaaaaaata ttatacgtaa
      121 ctggatctag agctgaatat ggaatagttc ggagactttt gacaatgcta agagaaactc
      181 cagaaataca gcttgatttg gcagttacag gaatgcattg tgataatgcg tatggaaata
      241 caatacatat tatagaacaa g
//
LOCUS       DAZFFE010000398          260 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZFFE010000398 DAZFFE010000000
VERSION     DAZFFE010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.401"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..176
                     /locus_tag="ACHJ69_005108"
     CDS             <1..176
                     /locus_tag="ACHJ69_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017869.1"
                     /translation="ELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIAQAYQQQIAE
                     SRRPEEETTPRET"
     gene            166..>260
                     /locus_tag="ACHJ69_005109"
     CDS             166..>260
                     /locus_tag="ACHJ69_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017870.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALG"
BASE COUNT           62 a           65 c           82 g           51 t
ORIGIN      
        1 aagagctggc gctgatgggc gaggattatc aggacatttt ccgccagcag gtcagggaat
       61 ctgcagagcg ggaaaaagcc ggactctcac gtccggtgtg gatagcgcag gcgtatcagc
      121 agcagatagc ggagagtcgc aggccggaag aggagacaac accacgtgag acgtaatctt
      181 tcacacatta ttgcagcagc attcaatgaa ccgctgcttc tggagcccgc ctatgcgcgg
      241 gttttctttt gcgcgctcgg
//
LOCUS       DAZFFE010000399          256 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZFFE010000399 DAZFFE010000000
VERSION     DAZFFE010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.402"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            22..204
                     /locus_tag="ACHJ69_005110"
     CDS             22..204
                     /locus_tag="ACHJ69_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017871.1"
                     /translation="MEKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGVAV
                     SPMLYGLAKLVIALKS"
BASE COUNT           72 a           46 c           76 g           62 t
ORIGIN      
        1 ttggataagg caagggagaa catggaaaag tataaacgaa ttctgttgag gttgactatg
       61 aaaaacggcc ttgaactgaa agcgcctgta actgatgaca tcagcagagc actggctttt
      121 gccattaagt gggtggcggt cggtgttgct gtgtccccga tgctgtatgg gctggcaaaa
      181 ctggtcattg cgttgaaatc gtgaagggag gattaagcat gtcagacaaa ctcataacgc
      241 tggcgaagat cctctg
//
LOCUS       DAZFFE010000400          252 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZFFE010000400 DAZFFE010000000
VERSION     DAZFFE010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.denovo.403"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>252)
                     /locus_tag="ACHJ69_005111"
     CDS             complement(<1..>252)
                     /locus_tag="ACHJ69_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO5017872.1"
                     /translation="LAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTYAVLSLFFCD
                     PDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGV"
BASE COUNT           63 a           71 c           65 g           53 t
ORIGIN      
        1 gacgcctccg gcaagtcctg ccgctttctg catcagcatt ttgtcctcat ccggcctctc
       61 cacctgctct tcctcatgcc gggggacaag cagactgaaa tcagagggat gcatatccgg
      121 atcgcaaaaa aacaggctga gtacagcgta cgtcagcccg gaaaaatgca tatccagctg
      181 ggtatcctga aaataatgcg tgcggtaaaa acggtgccag tcggcatatt cggtggatgt
      241 catcccggca ag
//
LOCUS       DAZFFE010000401         8422 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.guided.153, whole genome shotgun sequence.
ACCESSION   DAZFFE010000401 DAZFFE010000000
VERSION     DAZFFE010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.guided.153"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            63..1202
                     /locus_tag="ACHJ69_005112"
     CDS             63..1202
                     /locus_tag="ACHJ69_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO5017873.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESSAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRNSLGSIGGGNHFAELQQIDQIIDAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARINRQL
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            1199..1813
                     /gene="prfH"
                     /locus_tag="ACHJ69_005113"
     CDS             1199..1813
                     /gene="prfH"
                     /locus_tag="ACHJ69_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO5017874.1"
                     /translation="MILLQLSSAQGPEECCLAVRKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1870..3327)
                     /gene="pepD"
                     /locus_tag="ACHJ69_005114"
     CDS             complement(1870..3327)
                     /gene="pepD"
                     /locus_tag="ACHJ69_005114"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO5017875.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3588..4046
                     /gene="gpt"
                     /locus_tag="ACHJ69_005115"
     CDS             3588..4046
                     /gene="gpt"
                     /locus_tag="ACHJ69_005115"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO5017876.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            4138..5382
                     /gene="frsA"
                     /locus_tag="ACHJ69_005116"
     CDS             4138..5382
                     /gene="frsA"
                     /locus_tag="ACHJ69_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO5017877.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDGLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            5440..5841
                     /gene="crl"
                     /locus_tag="ACHJ69_005117"
     CDS             5440..5841
                     /gene="crl"
                     /locus_tag="ACHJ69_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO5017878.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAKVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(5880..6935)
                     /gene="phoE"
                     /locus_tag="ACHJ69_005118"
     CDS             complement(5880..6935)
                     /gene="phoE"
                     /locus_tag="ACHJ69_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO5017879.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASSLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            7224..8327
                     /gene="proB"
                     /locus_tag="ACHJ69_005119"
     CDS             7224..8327
                     /gene="proB"
                     /locus_tag="ACHJ69_005119"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO5017880.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8339..>8422
                     /locus_tag="ACHJ69_005120"
     CDS             8339..>8422
                     /locus_tag="ACHJ69_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5017881.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVL"
BASE COUNT         2036 a         2074 c         2203 g         2109 t
ORIGIN      
        1 tttttttctt gccctggatt cgtctgccat ttcctgattt ttattcataa atggaatggt
       61 acatgggcaa atatattcgt cccttatccg atgcggtatt taccatcgca tccgatgacc
      121 tgtggatcga aagttcagcg atccaacaat tacacaccac ggcaaattta cccaacatgc
      181 agcgcgttgt ggggatgcca gatttacatc ccggacgcgg ctacccgatt ggcgcagcgt
      241 tcttctctgt tggtcgtttt tacccggcac tggtcggcaa tgatatcggc tgcggcatgg
      301 cgctatggca aacagatatt ctcgctcgca aatataacgc cgataagttt gaaaagcgat
      361 tatcagatct ggatgacgtt gctgaagaaa gctggctgga ggaaaacctg ccgtcagcat
      421 ttgcacagca tccgtggcgc aactcgcttg gctccatcgg tggcggtaac cacttcgcag
      481 aactgcaaca gattgatcaa attatcgacg cggaactgtt tgcactggct ggtctggatg
      541 cgcagcatct gcaactgctg gttcatagcg gctcgagggg attggggcag tctattttac
      601 agcggcatat tgcctcgttt tcgcatcatg gtttgcctga aggcagtgac gacgcgctac
      661 ggtatattgc ggaacatgac gatgcgctgg cgtttgcgcg tattaatcgc cagctgatcg
      721 ctttgcgcat aatgcaacaa gttaaggcca ccggaagtcc ggttctggat gtggcgcata
      781 actttgttag cgcgtgccaa atcggcgatc aacagggctg gttgcatcgt aaaggcgcca
      841 cgccggatga caacggtctg gtgattattc ccggttcacg cggcgattac tcctggctgg
      901 ttaaacctgt tgcgaacgaa aaaacgctcc actcgctggc gcatggggcg gggcgtaaat
      961 gggggcgcac cgagtgtaaa gggcgtctgg cagcgaaata cacggcgacg caactctcgc
     1021 ggactgaact tggcagtcgg gtaatttgtc gcgataaaca actcatcttt gaagaagcgc
     1081 cacaagccta taaatcggct gaaagcgtgg tgcaatgtct ggtgctggct gggttaatta
     1141 ttcctgtcgc gcgactgcgc ccggtgctga cgctcaaaaa cagtggaggg aaaaaaggat
     1201 gatcttgcta caactctcct ctgctcaggg gccggaagaa tgttgtctcg cagtgagaaa
     1261 agcactggac aggctgatta aagaagctac ccgacaggac gtcgcggtaa cggtgctgga
     1321 aacagaaacg ggtcgctact ctgacacact gcgttcggcg ctgatttctc tggatggcga
     1381 taacgcatgg gctctaagcg aaagctggtg cggcactatt cagtggattt gtccgagtcc
     1441 gtatcggcct catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga
     1501 tgagcaggaa caatcggatg caatccgtta tgagacgctg cgttcgtcgg ggccgggcgg
     1561 tcaacatgtc aataaaaccg actcggcggt acgcgccacg catctggcat ccggtattag
     1621 cgtgaaggtt cagtcagagc gcagtcagca tgctaacaaa cggctggcgc gattactgat
     1681 tgcctggaag ctggaacaac agcaacagga aaatagcgcg gcgctgaaat cgcagcggcg
     1741 aatgttccat caccagattg aacgtggcaa cccgcgacga acgtttacag ggatggcttt
     1801 tatcgaaggg taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa
     1861 atcaaataat tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg
     1921 gccaacgctt tcgatatgaa cttgctcatc cggggagtgt ggaccggtga tggttggccc
     1981 gatagacacc atgtccattt ccggatacgg ttttttgaac aggccacatt cgaggcccgc
     2041 gtggataatc tggatgttcg gcgttttgtt gaacagacgc tgataggttt cacgtaccag
     2101 atgcattacc ggagagttag cgtccggctg ccagccagga tatgcgcctt ttgcttcggt
     2161 tttcgcacct gccagcttac ccagcgaatc cagcatgctc actacgtagt ctttaccgct
     2221 gtcgatcaga gaacggatca ggcagtgaat ttctacgtta ttgtcggtca tggtcaccac
     2281 accgacgttc agggaggttt cgaccacacc ttttgccacg tcggagttgc ggatcacacc
     2341 gttcggggtg gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc
     2401 tttatcgttc gcaacagagt ccagcagcaa tgccagattt ttctctttct ctgccagttc
     2461 gttttttagg atctcctgat aggtatccac cagagatttc agggcgtcga ctttatcagc
     2521 tgcgacagca atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt
     2581 gaaatcaata agacgcaggt ccagttcttc tgcatgaccc gccaggaagc gcaccaacag
     2641 tttgttggca ttacccaggc caacgtggat ttcaccgccg gagtgaccgc ctttcagacc
     2701 ttttaaggtt aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag
     2761 gttggaggtg aagtcgatac ctcccgcaca acccatgtag atttcacctt cttcttcgga
     2821 gtcggtgtta atcagaatat cagcctgcaa ccagttgctc tgtaagccaa aagcaccgtc
     2881 cataccggct tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc
     2941 gtcagccaga accgccagtg cagaggccat accaatgccg ttatccgcac ccagcgtggt
     3001 gccgcgtgct ttaacccatt cgccatcaat ataaggctgg ataggatctt tagtgaagtc
     3061 atgcacggtg tcgttatttt tttgcggcac catatcgagg tgggcctgca agacgaccgg
     3121 tttacgattt tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc
     3181 gcgttcgaca tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc
     3241 ttcatgatag gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg
     3301 tggagataat tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag
     3361 tattgcaggt cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat
     3421 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
     3481 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
     3541 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
     3601 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
     3661 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt
     3721 tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc
     3781 acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca
     3841 tcgttattga tgacctggta gataccggtg gtactgcggt tgcgattcgt gaaatgtatc
     3901 caaaagcgca ctttgtcacc atcttcgcaa aaccggccgg tcgtccgctg gttgatgatt
     3961 atgttgttga tattccgcaa aatacctgga tcgaacagcc gtgggatatg ggcgtcgtat
     4021 tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc
     4081 tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg
     4141 acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg
     4201 acgctagtcc gccgctttag tcacggcgca caactgcctg tgcagtcggc ccttgatggt
     4261 aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt
     4321 gacccacgtg aaatcctcga tgtccaggcg cgtattgtga tgagcgatgc cgaacgtacc
     4381 gacgatgatt tatacgatac ggtgattggc taccgcggcg gcaactggat ttatgagtgg
     4441 gctacccagg cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagc
     4501 ggtcgacact ggctgcatgc ggctacgttg tacaacattg ctgcctatcc tcatctgaaa
     4561 ggagatggcc tggccgagca agcgcaggct ttgtcaaacc gcgcctatga agaggccgct
     4621 cagcgtctgc cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc
     4681 accggctttt tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt
     4741 ggtctggatg cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc
     4801 ggcattgcga tgctgactat tgatatgccg tcggtgggct tttcttcaaa atggaagctc
     4861 acccaggact ccagcctgtt gcatcagcac gtcttaaagg cgctgcctaa cgtaccgtgg
     4921 gtagatcaca ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt
     4981 ctggcatacc ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat
     5041 accctgttga gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg
     5101 gcgagccgtt tggggatgca tgatgcttcc gacgaagcgt tgcgcgtgga gctgaatcgc
     5161 tattcattaa aagtgcaagg attgctggga cgtcgctgcc caacgccaat gttatcaggc
     5221 tactggaaga acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct
     5281 gctgacggta aattgttaga gatcccattt aacccggtgt atcggaattt tgacaaaggc
     5341 cttcaggaaa tcacagactg gatcgaaaaa cgtttgtgtt aaaaatttgc taaattttac
     5401 caatttggta aaacagttgc atcacaacag gagattgcaa tgacgttacc gagtggacac
     5461 ccgaagagca gattgatcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag
     5521 tgcgaagata atcgattctt tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca
     5581 ccggaagtgc gtgagttctg gggctggtgg atggaactgg aagcgcagga atcccgtttt
     5641 acctacagtt accagtttgg tctgttcgat aaagcaggcg actggacgag tgttcagata
     5701 aaagatgcca aagtggttga acgactggag cacaccctgc gtgaatttca cgagaagctg
     5761 cgtgagctgc tggcgacgct gaatctgaag ctggaaccgg cagatgattt tcgtgatgaa
     5821 ccggtgaagt taacggcgtg aatgaaatgt gccggatgca tcacatccgg caatatccat
     5881 taaaactgat aggtcatgcc aaccgcgaca atatcatcat tattaatatt caatttgtta
     5941 tcgctatcca gttggttgat tttataatca acaaacgctg acatattttt gttgaaataa
     6001 tacgtagcac caacatcgac ataattgacc agatcttcat caccgatgcc ctcaatatct
     6061 ttccctttcg ataagacata acccagcgat ggacgcagac caaagtcaaa ctggtattga
     6121 gcgaccgctt caaagttctg cgtcttattg gcaaagccgc cagagattgg cgtcatttta
     6181 cgtgtttcag aatagaaagt tgccagataa atattattgg catcgtattt cagacctgtt
     6241 gcccatgctt ctgcacgctt gcctgtgcca cggctttgca ggttctgctc gttggtgcga
     6301 tcagagttgg tataggctcc actgatggcg aaatcgctgc caccaaagtc atatgtcaat
     6361 gacgtgccga agccatcgcc gttttgcttt ttaacgtcgc ggttttcgtt tttcccttga
     6421 tattgcaggg ttaagttcag accatcgata acgccgaaga agtcggtgtt ccgatacgtc
     6481 gccagactgc tggcgcgttt ggtcataaag ttgtcggtct gcgccgagga gtcgccacca
     6541 aattccggga acatatcggt ccaggcttcc acgtcataca acgcgcccag gttacgacca
     6601 taatcgaaag aacccaaatc tttatatttc aacccggcaa aagcgagacg cgttttttgc
     6661 tgtgcagtat cactctccgc tttattaccg gcaaactcag cttcccaacg accataacca
     6721 gtcagttgat cgttaatttg tgtttcgcct ttaaaaccaa aacggatata actctggtcg
     6781 ccatctttac tgtcattatc actcatataa tgcatggctt taactttgcc atagacatcc
     6841 agtttattac cgtctttgtt atatatttct gcggcctgta cggatgcaga tgccacaatg
     6901 cccatcacca ctaatgccag agtgctcttt ttcattttca ttcctgattt taattaacgc
     6961 gcgaatattc agcgggagag tcccgttgaa aacaggaaag tttttaacct gagattgtta
     7021 aagatatatt acagattaat aatattctta aaatgtggta atttattaaa tctgtaataa
     7081 aagcgtaaac aactgccgct acgcttgctg atcccgcgca acaaaacgcc atgctttgct
     7141 cgcagatggt tggcaaccga cgacagtcct gctaaaacgt tcgtttgata tcattttttc
     7201 ctaaaattga atggcagaga atcatgagtg acagccagac gctggtggta aaactcggca
     7261 ccagtgtgct aacaggcgga tcgcgccgcc tgaaccgtgc ccatatcgtt gaacttgttc
     7321 gccagtgcgc gcagttacat gccgccgggc atcggattgt tattgtgacg tcgggtgcga
     7381 ttgccgccgg acgtgagcac ctggggtacc cggaattgcc agcgactatc gcttcaaaac
     7441 agctgctggc ggcggtaggg cagagtcgac tgattcaact gtgggaacag ctgttttcaa
     7501 tttacggcat tcatgtcggg cagatgttgc tgacccgtgc tgatatggaa gatcgtgaac
     7561 gcttcctgaa cgcccgtgat accctgcgtg cgttgctcga taacaatatc gttccggtaa
     7621 ttaatgagaa cgatgctgtc gctactgcag agattaaggt cggtgataac gataaccttt
     7681 ctgcgctggc ggcgattctg gcgggtgccg ataaactgtt gctgctgacc gatcaaaaag
     7741 gtttgtatac cgctgacccg cgcagcaatc cgcaggcaga actgattaaa gatgtgtacg
     7801 gcattgatga cgcactgcgc gcgattgccg gtgatagcgt ttcaggcctc ggaactggcg
     7861 gtatgagcac caaattgcag gccgctgacg tggcttgccg tgcgggtatc gacaccatta
     7921 ttgccgcggg cagcaagccg ggcgttattg gtgatgtgat ggaaggcatt tccgtcggta
     7981 cgctgttcca tgcccaggcg actccgcttg aaaaccgtaa acgctggatt ttcggtgcgc
     8041 cgcctgcggg tgaaatcacg gtagatgaag gggcaactgc cgccattctg gaacgcggca
     8101 gctccctgtt gccgaaaggc attaaaagcg tgactggcaa tttctcgcgt ggtgaagtca
     8161 tccgtatttg caacctcgaa ggtcgcgata tcgcccacgg cgtcagtcgt tacaacagcg
     8221 atgcattacg ccgtattgcc ggacaccact cgcaagaaat tgatgcaata ctgggatatg
     8281 aatacggccc ggttgccgtt caccgtgatg acatgattac ccgttaagga gctggccgat
     8341 gctggaacaa atgggcattg ccgcgaagca agcctcgtat aaattagcgc aactctccag
     8401 ccgcgaaaaa aatcgtgtgc tg
//
LOCUS       DAZFFE010000402         4371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.guided.200, whole genome shotgun sequence.
ACCESSION   DAZFFE010000402 DAZFFE010000000
VERSION     DAZFFE010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.guided.200"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            13..1146
                     /locus_tag="ACHJ69_005121"
     CDS             13..1146
                     /locus_tag="ACHJ69_005121"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO5017882.1"
                     /translation="MLKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            1235..1768
                     /gene="blc"
                     /locus_tag="ACHJ69_005122"
     CDS             1235..1768
                     /gene="blc"
                     /locus_tag="ACHJ69_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO5017883.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1765..2082)
                     /gene="sugE"
                     /locus_tag="ACHJ69_005123"
     CDS             complement(1765..2082)
                     /gene="sugE"
                     /locus_tag="ACHJ69_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO5017884.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2258..2404)
                     /gene="ecnB"
                     /locus_tag="ACHJ69_005124"
     CDS             complement(2258..2404)
                     /gene="ecnB"
                     /locus_tag="ACHJ69_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO5017885.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2515..2640)
                     /gene="ecnA"
                     /locus_tag="ACHJ69_005125"
     CDS             complement(2515..2640)
                     /gene="ecnA"
                     /locus_tag="ACHJ69_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO5017886.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(2692..3258)
                     /gene="efp"
                     /locus_tag="ACHJ69_005126"
     CDS             complement(2692..3258)
                     /gene="efp"
                     /locus_tag="ACHJ69_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO5017887.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3300..4328
                     /gene="epmB"
                     /locus_tag="ACHJ69_005127"
     CDS             3300..4328
                     /gene="epmB"
                     /locus_tag="ACHJ69_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO5017888.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1124 a         1126 c         1092 g         1029 t
ORIGIN      
        1 aaaccagacc ttatgctcaa aacgacactc tgcaccttat taattaccgc ctcttgctcc
       61 acatttgccg cccctcaaca aatcaacgat attgtgcatc gcacaattac cccgcttata
      121 gagcaacaaa agatccccgg tatggcggtg gcggtaattt atcagggtaa accttattac
      181 tttacctggg gctatgcgga catcgccaaa aagcagcccg tcacacagca aacgttgttt
      241 gagttaggtt cggtcagcaa aacatttacg ggcgtgcttg gtggcgacgc tattgctcga
      301 ggggaaatca agttaagcga tcccacaaca aaatactggc ctgaacttac cgctaaacag
      361 tggaatggga tcacactatt acatctcgca acctacaccg ctggcggcct gccattgcag
      421 gtgccggatg aggtgaaatc ctcaagcgac ttgctgcgct tctatcaaaa ctggcagcct
      481 gcatgggctc caggaacaca acgtctgtat gccaactcca gtatcggttt gttcggcgca
      541 ctggctgtga agccgtctgg tttgagtttt gagcaggcga tgaaaactcg tgtcttccag
      601 ccactcaaac tcaatcatac gtggattaat gtaccgtccg cagaagaaaa gaactacgcc
      661 tggggatatc gcgaaggtaa ggcagtacat gtttcgccag gggcgttaga tgctgaagct
      721 tatggtgtga agtcgaccat tgaagatatg gcccgctggg tgcaaagcaa tttaaaaccc
      781 cttgatatca atgagaaaac acttcaacaa gggatacaac tggcacaatc tcgctactgg
      841 caaaccggcg atatgtatca gggtctgggc tgggaaatgc tggactggcc ggtaaatcct
      901 gacatcatca ttaacggcag tgacaataaa attgcactgg cagcacgccc cgtaaaaccg
      961 attacgcccc caactcctgc agtacgcgca tcatgggtac ataaaacggg ggcgaccggc
     1021 ggatttggta gctatgtcgc gtttattcca gaaaaagagc tgggtatcgt gatgctggct
     1081 aacaaaaact accccaatcc agcgagagtc gccgccgcct ggcagattct caacgctcta
     1141 cagtaaaatt tcatcgggtc cgaattttcg gaccttttct ccgcttttcc ttgctgtcat
     1201 ctacacttag aaaaaaacca gtaaggaaac atttatgcgc ctgctccctc tcgttgccgc
     1261 agcgacagct gcatttctgg tcgttgcctg cagttcccct acgccgccgc gtggcgtgac
     1321 cgtagtgaat aatttcgacg ctaaacgtta tctcggtacc tggtatgaga ttgcccgttt
     1381 tgatcaccgc tttgaacgtg gactggaaaa agtcaccgca acatacagcc tgcgtgatga
     1441 cggcggcctg aatgtcatta ataaaggcta taaccctgac agagaaatgt ggcagcagag
     1501 tgaagggaaa gcgtacttta ccggcgcacc aactcgcgct gcgctgaaag tgtcattctt
     1561 tggtcctttc tatggcggtt ataacgttat tgcactcgat cgggaatacc gccatgcgct
     1621 ggtttgcggc ccggaccgcg actacctgtg gatactctcc cgcacgccaa ccatttctga
     1681 cgaagtgaaa caggagatgc tggcagtcgc gacccgggaa gggtttgatg tcagtaagtt
     1741 tatttgggta cagcagcccg gtagttaatg agtgctgagt ttcagaccaa taatccccaa
     1801 tacgattaac gccagactcg ccaggcgcat cgggttagcg gactcaccga gcagcacaat
     1861 gccggtgatg gccgcgccga ctgcgccgat acccgtccac acggcataag ccgtccctac
     1921 tggtaacgat ttcatcgccc aggcaagtag cgccatactg acaatcatcg ccgtcacggt
     1981 aataacactt ggcgtcaaac gactaaagcc gtgagtatat ttcaggccaa cggcccatac
     2041 cacttccagc agaccagcaa taactaagat aatccaggac atatcaggct ccagaaaatt
     2101 ggggccgtcc ccggtgaaaa gacgcgtttg caggtcgtcc tgcaaagctg atggttgaac
     2161 ggatattttg ccaggtggca ggggaatttc aactattacc tatgtaacta tcgcagaaaa
     2221 aacaaacggc acgacacagc tgtcgtaccg ttattgctta ttgctgcgct ttcgttgctg
     2281 cgccagaaat cgcgttaccg ccatcagaaa tgtcttcacc aacgccacgc gtggtgttgc
     2341 aggcagttaa tactgttgaa agcaccagaa cagaaaagat cgctgcaatt gtcttcttca
     2401 ccataacgtc ttccttttag gtaatgttat ttatgtttgc ctatagcaaa ttaataatag
     2461 acaacatcgc caaaaatgat ggaaatctga tgatttttcg gaggagaaga aaaattagct
     2521 ggcagcgcgg gagatggagt tgccgagatg tttgatatct tcgccgaaac cgcgagcggt
     2581 gttacagccc gtgagcagcg cgctggcaag caaaacaaga acgataaggc gtttcatcat
     2641 ctctgacctg aaaaagtgat ggtgcagcct gcaggccgca ccacaaccgc attacttcac
     2701 gcgagagacg tattcaccag agcgggtatc cactttgatg acttcgccga tctgcacgaa
     2761 cagcggaact ttaaccacag cgccagtaga cagggtagcc ggtttgccgc cagtacctgc
     2821 ggtgtcacct ttcaggcccg gatcggtatc aacgatttcc agttcaacga agttcggcgg
     2881 agtaacggag atcggctgac cattccacag agttacgata cactctgcct gatccagcaa
     2941 ccatttagcg ttgtcgccaa ttgcttttgc gtcagcagac agctgctcga aagtttcgtt
     3001 gttcatgaag tgccagaact caccgtcgtt gtacaggtaa gtcaggttca tatcgacaac
     3061 atcagcgcct tcagcagaat cagtagattt gaaggttttt tctacgcgag taccggtcag
     3121 cagacgacgc agtttaacgc gagcaaatgc ctggccttta cccggtttta cgaattcact
     3181 cgcttcaacc gcgtaaggtt cgccgtctaa catgatttta agaccagcac gaaaatcgtt
     3241 gctatagtac gttgccataa ggccctctga aatttgttaa ttggtagcta agccacaaaa
     3301 tggcgcatat tgtaacccta aataccccat ccagagaaga ttggttaacg caacttgccg
     3361 atgttgtgac cgatcctgat gaacttctgc gtcttttgaa tatagacgcg gacgaaaaac
     3421 tgttggccgg acgcagcgcc aaaaagctgt ttgccctgcg tgtgccccgc tcatttatcg
     3481 atcgcatgga gaaaggcaat ccggacgatc ctcttttgcg tcaggtactt acctcgcaag
     3541 atgagtttgt cgtcgcgccc ggattctcca ccgacccgct ggaagaacag catagcgtag
     3601 tgcctggttt gttgcataaa taccacaacc gggcgctttt gctggtcaaa ggcggctgcg
     3661 cggtaaattg ccgctattgc ttccgtcgcc acttccccta tgccgaaaat cagggcaaca
     3721 agcgtaactg gcagactgca cttgagtatg ttgctgcgca tccggaactg gacgagatga
     3781 ttttctccgg cggcgatccg ctgatggcga aagatcacga gctggactgg ttgctcacac
     3841 aactggaagc catcccgcat atcaaacgtc tgcggattca cagccgtctg ccgattgtga
     3901 tcccggcacg catcacagat gcgctggttg aacgcttttc tcattctacg ctgcaaatct
     3961 tgctggtgaa tcacatcaac catgccaatg aggtagatga aacattccgt caggcgatgg
     4021 ctaagttgcg ccgggtgggc gttactttgc tgaaccagag cgttctgtta cgtggtgtga
     4081 acgataacgc gcaaacgctg gcaaacctga gtaatgcgtt gttcgatgcg ggcgtgatgc
     4141 cgtattacct gcatgtgctc gataaagtac agggcgcggc gcattttatg gtgagtgatg
     4201 acgaagcacg gcagattatg cgtgagttgc tgacattggt gtcgggatat ctggtgccta
     4261 aactggcgcg agaaattggc ggcgaaccca gcaaaacgcc gctggatctc cagctacgcc
     4321 agcagtaact gattaaacag ggaagtttaa tctttgcccg ttgcataacc t
//
LOCUS       DAZFFE010000403         1659 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104F7260
            SAMN43267443-rid23065963.guided.256, whole genome shotgun sequence.
ACCESSION   DAZFFE010000403 DAZFFE010000000
VERSION     DAZFFE010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267443
            Sequence Read Archive: SRR30307180
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350498.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350498.1
            Genome Coverage       :: 111x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:12:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,129
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,802
            CDSs (with protein)               :: 4,802
            Genes (RNA)                       :: 91
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 110 of 236
            Pseudo Genes (incomplete)         :: 121 of 236
            Pseudo Genes (internal stop)      :: 54 of 236
            Pseudo Genes (multiple problems)  :: 40 of 236
            Pseudo Genes (short protein)      :: 1 of 236
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267443-rid23065963.guided.256"
                     /serotype="O117:H 7"
                     /isolate="2104F7260"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            285..1233
                     /gene="stxA1a"
                     /locus_tag="ACHJ69_005128"
                     /pseudo
     CDS             285..1233
                     /gene="stxA1a"
                     /locus_tag="ACHJ69_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
     gene            1243..1512
                     /gene="stxB1a"
                     /locus_tag="ACHJ69_005129"
     CDS             1243..1512
                     /gene="stxB1a"
                     /locus_tag="ACHJ69_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO5017889.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          444 a          286 c          401 g          528 t
ORIGIN      
        1 gcgtaacagc attttgctct acgagtttgc cagcctcccc cagtggctgg cttttttatg
       61 tccgtaacat cctgtgtatc aataaatgtt gttgtctacg tacgtcaagt agccgcatga
      121 gatctgacca gatatgttaa ggttgcagct ctctttgaat ataattatca ttttcattac
      181 gttattgtta cgtttatccg gtgcgccgta aaacgccgtc cttcaggggg tggaggatgt
      241 caagaatata gttatcgtat ggtgctcaag gagtattgtg taatatgaaa ataattattt
      301 ttagagtgct aacttttttc tttgttatct tttcagttaa tgtggttgcg aaggaattta
      361 ccttagactt ctcgactgca aagacgtatg tagattcgct gaatgtcatt cgctctgcaa
      421 taggtactcc attacagact atttcatcag gaggtacgtc tttactgatg attgatagtg
      481 gcacagggga taatttgttt gcagttgatg tcagagggat agatccagag gaagggcggt
      541 ttaataatct acggcttatt gttgaacgaa ataatttata tgtgacagga tttgttaaca
      601 ggacaaataa tgttttttta tcgctttgct gatttttcac atgttacctt tccaggtaca
      661 acagcggtta cattgtctgg tgacagtagc tataccacgt tacagcgtgt tgcagggatc
      721 agtcgtacgg ggatgcagat aaatcgccat tcgttgacta cttcttatct ggatttaatg
      781 tcgcatagtg gaacctcact gacgcagtct gtggcaagag cgatgttacg gtttgttact
      841 gtgacagctg aagctttacg ttttcggcaa atacagaggg gatttcgtac aacactggat
      901 gatctcagtg ggcgttctta tgtaatgact gctgaagatg ttgatcttac attgaactgg
      961 ggaaggttga gtagtgtcct gcctgactat catggacaag actctgttcg tgtaggaaga
     1021 atttcttttg gaagcattaa tgcaattctg ggaagcgtgg cattaatact gaattgtcat
     1081 catcatgcat cgcgagttgc cagaatggca tctgatgagt ttccttctat gtgtccggca
     1141 gatggaagag tccgtgggat tacgcacaat aaaatattgt gggattcatc cactctgggg
     1201 gcaattctga tgcgcagaac tattagcagt tgagggggta aaatgaaaaa aacattatta
     1261 atagctgcat cgctttcatt tttttcagca agtgcgctgg cgacgcctga ttgtgtaact
     1321 ggaaaggtgg agtatacaaa atataatgat gacgatacct ttacagttaa agtgggtgat
     1381 aaagaattat ttaccaacag atggaatctt cagtctcttc ttctcagtgc gcaaattacg
     1441 gggatgactg taaccattaa aactaatgcc tgtcataatg gagggggatt cagcgaagtt
     1501 atttttcgtt gactcagaat agctcagtga aaatagcagg cggagattca taaatgttaa
     1561 atacatctca attcagtcag ttgttgccgg tctgataata gatgtgttag aaaatttctg
     1621 catggtgaat ccccctgtgc ggaggggcga ctggtgaac
//
